2025-11-09
ENA first public
ERC000011
ENA-CHECKLIST
The Open University
INSDC center name
public
INSDC status
ERS27212947
SRA accession
E-MTAB-16057:Sample 6
Submitter Id
ArrayExpress
broker name
LNCAP
cell line
not collected
collection date
human
common name
Protocols: LNCaP human prostate cancer cells were cultured under chronic hypoxia (2% O₂) in RPMI-1640 medium (Gibco #21875034) supplemented with 10% fetal bovine serum (ThermoFisher #26140079) and Penicillin/Streptomycin/Amphotericin B (ThermoFisher #15140122). Cells were harvested by trypsinization at experimental time points (Day 3, Day 6, Day 9), counted using a Luna-FL cell counter, and assessed for viability via trypan blue exclusion. Samples were maintained at 2% O₂ until processing. Single-cell suspensions were prepared following the 10x Genomics Chromium Next GEM Single Cell 3′ Kit v3.1 user guide. RNA molecules were captured within Gel Beads-in-Emulsion (GEMs) using barcoded oligo-dT primers. Reverse transcription within droplets generated cDNA tagged with unique molecular identifiers (UMIs) and cell barcodes. No separate bulk RNA extraction step was performed. cDNA synthesis and library preparation were performed using the 10x Genomics Chromium Next GEM Single Cell 3′ Kit v3.1 (PN-1000268/1000269) together with the 3′ Feature Barcode Kit (PN-1000262), Chromium Next GEM Chip G Single Cell Kit (PN-1000127/1000120), and Dual Index Kit TT Set A (PN-1000215). Libraries were constructed according to the manufacturer’s instructions at the Babraham Institute Genomics Facility.
description
prostate adenocarcinoma
disease
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
prostate gland
organism part
Homo sapiens
scientific_name
Sample 6
title