2025-11-10
ENA first public
ERC000011
ENA-CHECKLIST
Institute of Pharmacology and Toxicology, University of Bonn
INSDC center name
public
INSDC status
ERS27117044
SRA accession
E-MTAB-15819:RT WT
Submitter Id
ArrayExpress
broker name
not collected
collection date
house mouse
common name
Protocols: Frozen adipose tissue was minced into 1–3 mm pieces on ice. The minced tissue was homogenized in a Dounce homogenizer on ice in 0.1% CHAPS in CST buffer with 0.2U Rnase inhibitor, lysed for 5 min, and quenched by 1% BSA in PBS with 0.2U Rnase inhibitor. The homogenized adipose tissue was filtered through a 40 μm cell strainer and centrifuged at 500 x g for 5 min at 4°C. The pellet was resuspended and washed with 1% BSA in PBS with 0.2U Rnase inhibitor. The nuclei suspension was centrifuged again at 500 x g for 5 min at 4°C, resuspended in 1% BSA containing PBS with 1U Rnase inhibitor, and filtered through a 20 μm strainers.   none cold exposure Nuclei were loaded on a 10x Chip G directly 10x 3' V3.1 library construction
description
ChREBP-expressing control (Cre-)
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
brown adipose tissue
organism part
Mus musculus
scientific_name
B6
strain
RT WT
title