2025-11-12
ENA first public
ERC000011
ENA-CHECKLIST
Institute of Immunobiology, Kantonsspital St. Gallen, St. Gallen, Switzerland
INSDC center name
public
INSDC status
ERS18403465
SRA accession
E-MTAB-13891_2:Sample 24
Submitter Id
8
week
age
ArrayExpress
broker name
fibroblast
cell_type
not collected
collection date
house mouse
common name
Protocols: For stromal cell isolation from peripheral (inguinal, brachial and axillary) and mesenteric lymph nodes, tissues were disrupted into small pieces using small needles and collected in RPMI 1640 medium containing 2% FCS, 20 mM HEPES pH 7.2 (Lonza), 0.16 mg/ml collagenase P, Dispase 30 μg/ml (Roche) and 25 μg/ml DNase I. The dissociated tissue was incubated for 45 min at 37 °C, during the incubation time the tissue was resuspended and supernatant was collected every 15 min.  Enrichment of stromal cells was performed by incubating the cell suspension with MACS anti-CD45 and anti-TER119 microbeads (Miltenyi Biotec) and passing it through MACS LS columns (Miltenyi Biotec). Single-cell suspensions were stained for further cell sorting. Stromal cells were sorted for CD31-EYFP+ cells and were filled up with CD31-EYFP- cells. Cell sorting was performed using a BD FACSMelody Cell Sorter and the FACSChorus (v.1.3) software (BD Biosciences). cDNA library generation was performed following the established commercial protocol for Chromium Single Cell 3’ Reagent Kit (v3 Chemistry).
description
adult
developmental stage
C57BL/6N-Tg(Ccl19-tTA)xLC1xR26-EYFP
genotype
not collected
geographic location (country and/or sea)
pooled
individual
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
mesenteric lymph node
organism part
Mus musculus
scientific_name
mixed
sex
C57Bl/6N
strain
Sample 24
title