2025-11-12
ENA first public
ERC000011
ENA-CHECKLIST
Centre for Regenerative Medicine, University of Edinburgh
INSDC center name
public
INSDC status
ERS23654661
SRA accession
E-MTAB-14862:WT_1
Submitter Id
P14
day
age
ArrayExpress
broker name
undifferentiated spermatogonium
cell_type
not collected
collection date
house mouse
common name
Protocols: FACS-purified P14 undifferentiated spermatogonia (DAPI -ve, CD45 -ve, CD51-ve, c-Kit -ve, CD9 +ve) DNA from spermatogonia was isolated by Proteinase K digest (10 mM Tris-HCl pH 8, 5 mM EDTA , 1% SDS, 0.3 M Na-acetate, 0.2 mg/ml proteinase K) overnight, followed by 2x phenol/chloroform/isoamylalcohol (25:24:1, buffered at pH 8, Sigma-Aldrich) extraction and 2x chloroform extraction. The DNA was precipitated at -20˚C after addition of 1/10 volume 3M Na-acetate, 10µg linear acrylamide (Invitrogen) and 1 volume of isopropanol, washed 2x with 70% ethanol, and solubilized in 5mM Tris pH8. Methyl-seq libraries were prepared using the NEBnext Enzymatic Methyl-seq kit (NEB E7120) according to the manufacturer’s instructions
description
juvenile stage
developmental stage
Spocd1: +/+
genotype
not collected
geographic location (country and/or sea)
4
individual
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
testis
organism part
1
replicate
Mus musculus
scientific_name
male
sex
B6CBAF1/C57BL6N
strain
WT_1
title