SAMN00154785
SAMN00154785
External Id
LIBEST_000170
External Id
C.S.I.C.
INSDC center name
2001-06-08T00:00:00Z
INSDC first public
2025-08-18T21:06:27.126Z
INSDC last update
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
ERS25789503
SRA accession
Scarlet Globe
cultivar
A primary libray was constructed from RNA from segments of radish roots that had been cultured in 30 5M IAA for 24 hours to induce the formation of new lateral root primordia. The segments were 1 cm in lenght and located 0.5 cm from the root tip so that they contained mature root cells but had not initiated any lateral primordia at the start of the culture period. The cDNA was directionally cloned into lambda GEM-2 after priming with an XbaI-oligo(dT) primer adaptor. ATR-24h is a subtracted library that was prepared from this primary library as described by Palazzolo et al. (Neuron 3: 527-539, 1990) using RNA from non-treated roots and roots treated for 4h as driver.
description
Raphanus sativus var. sativus
http://purl.obolibrary.org/obo/NCBITaxon_240228
organism
EcoRI
re_1
XbaI
re_2
ATR-24H
title
Phage
v_type
sativus
variety
lambda GEM-4
vector