SAMN00153410
LIBEST_021749
External Id
SAMN00153410
External Id
J. Craig Venter Institute
INSDC center name
2007-08-22T00:00:00Z
INSDC first public
2025-08-18T21:06:27.028Z
INSDC last update
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
ERS25789508
SRA accession
Early Scarlet Globe
cultivar
Total RNA from whole seedling (with 1 set of true leaves), buds, and anthers were pooled together. Double strand cDNA were synthesized from pooled RNA using SMART technology (Clontech). Then the prepared cDNA was normalized by cDNA denaturation/reassociation, treatment by duplex-specific nuclease(DSN) and amplification of normalized fraction by PCR. The normalized cDNA was then digested with SfiI, fractioned, directionally ligated into pDNR-LIB (Clontech) and electroprated into GC10 competent cell (Gene Choice). Sequences were generated from 5' and 3' ends of clones.
description
Raphanus sativus var. sativus
http://purl.obolibrary.org/obo/NCBITaxon_240228
organism
SfiIA
re_1
SfiIB
re_2
whole seedling (with 1 set of true leaves), buds, and anthers
tissue-type
RS2(RS)
title
sativus
variety
pDNR-LIB(Clontech)
vector