SAMN00154784
LIBEST_000169
External Id
SAMN00154784
External Id
C.S.I.C.
INSDC center name
2001-06-08T00:00:00Z
INSDC first public
2025-08-18T21:06:27.100Z
INSDC last update
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
ERS25789506
SRA accession
Scarlet Globe
cultivar
A primary libray was constructed from RNA from segments of radish roots that had been cultured in 30 5M IAA for 4 hours to induce the formation of new lateral root primordia. The segments were 1 cm in lenght and located 0.5 cm from the root tip so that they contained mature root cells but had not initiated any lateral primordia at the start of the culture period. The cDNA was directionally cloned into lambda GEM-2 after priming with an XbaI-oligo(dT) primer adaptor. ATR-4h is a subtracted library that was prepared from this primary library as described by Palazzolo et al. (Neuron 3: 527-539, 1990) using RNA from non-treated roots as driver.
description
Raphanus sativus var. sativus
http://purl.obolibrary.org/obo/NCBITaxon_240228
organism
EcoRI
re_1
XbaI
re_2
ATR-4H
title
Phage
v_type
sativus
variety
lambda GEM-4
vector