E-MTAB-15840:Sample 9
2025-11-14
ENA first public
ERC000011
ENA-CHECKLIST
UCLA, Devis Geffen school of Medicine, Department of Medicine, / Cardiology
INSDC center name
public
INSDC status
ERS27123402
SRA accession
E-MTAB-15840:Sample 9
Submitter Id
66
year
age
ArrayExpress
broker name
not collected
collection date
human
common name
Protocols: The blood was collected from the individual for 3 visits and timepoints, PBMC were isolated according to the manufacture's protocol  RNA were isolated from the PBMC using RNeasy Mini Kit (Qiagen, Valencia, CA). The quality of the total RNA assessed using NanoDrop® ND-8000 spectrophotometer (NanoDrop Technologies, Wilmington, DE) and Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA) . All RNA samples should display a concentration above 50 ng/µl, purity - 260/280 ~ 2.0., integrity - RIN > 8.0 and average > 8.5 The library prepared with Illumina TruSeq RNA kit according to the manufacturer’s instructions (Illumina, San Diego, CA). Additionally, the KAPA RNA HyperPrep Kit with RiboErase (HMR) (Roche, Pleasanton, CA) will be used in order to provide improved coverage of precursor mRNAs and noncoding RNA
description
Recovery
developmental stage
normal
disease
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
PBMC
organism part
Homo sapiens
scientific_name
male
sex
Sample 9
title
3
visit