E-MTAB-15888:Oryza sativa, 100µM phosphinothricin for 48h, Rep 1
2025-11-14
ENA first public
ERC000011
ENA-CHECKLIST
Temasek Life Sciences Laboratory
INSDC center name
public
INSDC status
ERS27181189
SRA accession
E-MTAB-15888:Oryza sativa, 100µM phosphinothricin for 48h, Rep 1
Submitter Id
79
day
age
ArrayExpress
broker name
not collected
collection date
Asian cultivated rice
common name
Japonica
cultivar
Protocols: Leaf samples were blotted dry and snap-frozen in liquid nitrogen Rice plants were grown in glasshouse under natural light until maturity. Rice leaf discs were mature leaf sections were floated abaxial side down in liquid ½ MS solution (pH 5.7, no sucrose) containing the chemical of interest and incubated for 48h at 28°C, under 24h light. Frozen samples are milled in extraction buffer and total RNA extracted using the QIAGEN Plant RNeasy kit Directional mRNA library preparation (poly A enrichment)
description
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Oryza sativa
organism
Oryza sativa
organism
leaf
organism part
1
replicate
Oryza sativa
scientific_name
100µM phosphinothricin treatment for 48h
stimulus
Oryza sativa cv. Nipponbare
strain
Oryza sativa, 100µM phosphinothricin for 48h, Rep 1
title