E-MTAB-16017:RR_Si-con_24hr_1_S215
2025-11-14
ENA first public
ERC000011
ENA-CHECKLIST
Ewha womans university
INSDC center name
public
INSDC status
ERS27181443
SRA accession
E-MTAB-16017:RR_Si-con_24hr_1_S215
Submitter Id
ArrayExpress
broker name
Hs 578T
cell line
epithelial cell
cell type
not collected
collection date
human
common name
Protocols: Hs578T parental and radioresistant (RR) cells were harvested 24 hours after transfection for RNA extraction. Cells were washed twice with cold PBS and collected directly from culture dishes using cell scrapers, ensuring consistent handling across all samples. Hs578T parental and RR cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 25 mM HEPES. Cells were maintained at 37 °C in a humidified atmosphere of 95% air and 5% CO₂. For gene knockdown experiments, cells were transfected with control siRNA (sc-37007) or AR siRNA (sc-29204) using Lipofectamine RNAiMax (Invitrogen, 13778-150) following the manufacturer’s instructions. siRNAs were diluted to a final concentration of 10 nM in Opti-MEM and incubated for 15 min with Lipofectamine RNAiMax at a 1:1 ratio before being added to the cells. Total RNA was extracted using the Illumina TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, CA, USA) according to the manufacturer’s protocol. RNA integrity was verified using an Agilent 2100 Bioanalyzer before library preparation. RNA-seq libraries were constructed using the Illumina TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, CA, USA). Poly(A)+ mRNA was purified from total RNA, fragmented, and reverse transcribed to cDNA. After adapter ligation and PCR amplification, libraries were quantified and quality-checked prior to sequencing.
description
adult
developmental stage
triple-negative breast cancer
disease
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
breast
organism part
Homo sapiens
scientific_name
RR_Si-con_24hr_1_S215
title