E-MTAB-16016:TLR1-1805TT PBMCs_Restim_rep1
2025-11-14
ENA first public
ERC000011
ENA-CHECKLIST
Tufts
INSDC center name
public
INSDC status
ERS27210553
SRA accession
E-MTAB-16016:TLR1-1805TT PBMCs_Restim_rep1
Submitter Id
ArrayExpress
broker name
primary cell line
cell line
mononuclear cell
cell type
not collected
collection date
human
common name
Protocols: Cells were harvested following stimulation (16hr) and restimulation (6hr) for total RNA extraction. PBMCs were maintained in RPMI 1640 supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS) in humidified atmosphere with 5% CO2 at 37C. Control cells were given growth medium at stimulation and restimulation. Stimulated and Restimulated cells were treated with live B. burgdorferi. Total RNA was extracted using Rneasy Plus Micro Kit (Qiagen). Libraries were prepared using NEBNext Ultra-II Directional RNA Library Prep Kit (Illumina).
description
adult
developmental stage
none
disease
TLR1-1805TT
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
blood
organism part
Homo sapiens
scientific_name
Borrelia burgdorferi
stimulus
TLR1-1805TT PBMCs_Restim_rep1
title