characteristics.text: 2025-11-15
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: Department of Gastrointestinal Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS27114169
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-15816:LPSITA1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: primary cell line
characteristics.characteristic: cell line
characteristics.text: bone marrow macrophage
characteristics.characteristic: cell type
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: WT BMDMs were inoculated in 6-well plates. First, they were pretreated with 10 mM itaconate or mesaconate for 4 hours, then treated with 10ng/ml LPS for 3 hours. After that, the culture medium was discarded and 1ml of RNAiso Plus was added to each well. The samples were stored at -80℃ and quantitatively analyzed using the sequencing platform of the Beijing Institute of Genomics. RNA was harvested using Rneasy mini plus kit (Qiagen). 1.3 ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared using SMARTER mRNA-Seq Library Prep Kitfollowing manufacturer's protocols.
characteristics.characteristic: description
characteristics.text: inflammatory disease
characteristics.characteristic: disease
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: bone marrow
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: C57BL/6J
characteristics.characteristic: strain
characteristics.text: LPSITA1
characteristics.characteristic: title