characteristics.text: SAMN00770594
characteristics.characteristic: External Id
characteristics.text: Jinan University
characteristics.characteristic: INSDC center name
characteristics.text: 2013-01-07T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2025-08-26T21:23:39.609Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS285063
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS285063
characteristics.characteristic: SRA accession
characteristics.text: Eustigmatos cf. polyphem is formarly determined as Myrmecia bisecta, which Taxon ID is 41462. Total RNA was isolated using TRIzol reagent (Invitrogen) according to the manufacturer’s protocol from pure axenic cultures of E. cf. polyphem.The samples for transcriptome analysis were prepared using Illumina's kit following manufacturer's recommendations. Briefly, mRNA was purified from 6 μg of total RNA using oligo (dT) magnetic beads. Following purification, mRNA is fragmented into small pieces using divalent cations under elevated temperature and the cleaved RNA fragments were used for first strand cDNA synthesis using reverse transcriptase and random primers. This was followed by second strand cDNA synthesis using DNA polymerase I and RNaseH. These cDNA fragments then went through an end repair process and ligation of adapters. These products were purified and enriched with PCR to create the final cDNA library.At last, the library was sequenced using Illumina GA IIX.
characteristics.characteristic: description
characteristics.text: Vischeria cf. polyphem
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_1132302
characteristics.characteristic: organism
characteristics.text: Illumina RNA-seq results of Eustigmatos cf. polyphem
characteristics.characteristic: title