characteristics.text: 2025-11-15
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: ULB
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS27239236
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-13223_2:Sample 1
characteristics.characteristic: Submitter Id
characteristics.text: 5
characteristics.unit: month
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: leukocyte
characteristics.characteristic: cell type
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: zebrafish
characteristics.characteristic: common name
characteristics.text: Protocols: FACS-sorting of cd45:Dsred cells. Brains from adult Tg(p2ry12:GFP; cd45:DsRed) fish (n=3), were dissected in 0.9X Dulbecco′s Phosphate Buffered Saline (DPBS) were triturated and treated with Liberase TM at 33°C for 30-45 minutes, fully dissociated using a syringe with a 26G needle and washed in 2% fetal bovine serum diluted in 0.9X DPBS. Cell suspensions were centrifuged at 290g 4ºC 10 min and filtered through a 40µm nylon mesh. Just before flow cytometry analysis, using calcein violet to exclude dead cells (1µM, Thermo Fisher). Flow cytometry acquisition and cell sorting was performed on a FACS ARIA II (Becton Dickinson).  RNA Illumina
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: pool of 3 fish
characteristics.characteristic: individual
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Danio rerio
characteristics.characteristic: organism
characteristics.text: Danio rerio
characteristics.characteristic: organism
characteristics.text: brain
characteristics.characteristic: organism part
characteristics.text: Danio rerio
characteristics.characteristic: scientific_name
characteristics.text: both
characteristics.characteristic: sex
characteristics.text: AB
characteristics.characteristic: strain
characteristics.text: Sample 1
characteristics.characteristic: title