characteristics.text: 2025-11-15
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: Centre for Metabolism, Obesity and Diabetes Research (MODR), Department of Medicine, McMaster University
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS27116928
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-15818:HCT116 ASCC3-KO cells, KO, biol rep 2
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: HCT116
characteristics.characteristic: cell line
characteristics.text: colorectal cancer cell
characteristics.characteristic: cell type
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: HCT116 parental (WT) and ASCC3-KO cells were maintained at 37°C with 5% CO₂ in DMEM supplemented with 10% fetal bovine serum, L-glutamine, non-essential amino acids, 100 U/mL penicillin and 0.1 mg/mL streptomycin; cultures were routinely screened for mycoplasma by DAPI staining. Cells were harvested for RNA extraction. Total RNA was extracted with TRIzol and purified using the RNeasy Mini Kit (Qiagen, cat. no. 74106) per manufacturer’s instructions; RNA integrity was confirmed by BioAnalyzer. Libraries were prepared using the NEBNext® Ultra™ II Directional RNA Library Prep Kit (New England Biolabs) with poly(A) selection, following the manufacturer’s protocol.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: colorectal carcinoma
characteristics.characteristic: disease
characteristics.text: ASCC3-KO
characteristics.characteristic: genotype
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: DMEM, 10% FBS, L-glutamine, non-essental amino acids, 100U/mL penicillin, 0.1 mg/mL streptomycin
characteristics.characteristic: growth condition
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: colon
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: HCT116 ASCC3-KO cells, KO, biol rep 2
characteristics.characteristic: title