characteristics.text: 2025-11-15
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: University of Hertfordshire
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS27232487
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-16084:WT_3
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: Protocols: Synchronized populations of C. elegans (wild-type and smn-1 mutants) were cultured on NGM plates seeded with OP50 E. coli and collected at the early L4 stage for RNA extraction. Total RNA was isolated from synchronised populations of C. elegans using TRIzol reagent, following standard protocols. Briefly, animals were washed and pelleted by centrifugation, then lysed in TRIzol (ThermoFisher). Samples were subjected to repeated freeze-thaw cycles in liquid nitrogen and a 37°C heating block to ensure complete lysis, followed by phase separation with chloroform (ThermoFisher). The aqueous phase was collected, and RNA was precipitated with isopropanol (ThermoFisher), washed with 75% ethanol, and air-dried before resuspension in nuclease-free water. RNA concentration and purity were determined spectrophotometrically. RNA integrity was assessed by electrophoresis on a 1% agarose-TBE bleach gel, and samples displaying clear 28S, 18S, and 5S rRNA bands with a 28S:18S ratio of ~2:1 were considered high quality. mRNA was purified using poly-T oligo-attached magnetic beads, fragmented, and used for first- and second-strand cDNA synthesis. After end repair, adaptor ligation, and size selection (150–200 bp), PCR amplification was performed and library quality was verified on the Agilent Bioanalyzer.
characteristics.characteristic: description
characteristics.text: L4 larva
characteristics.characteristic: developmental stage
characteristics.text: +/+
characteristics.characteristic: genotype
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Caenorhabditis elegans
characteristics.characteristic: organism
characteristics.text: Caenorhabditis elegans
characteristics.characteristic: organism
characteristics.text: whole organism
characteristics.characteristic: organism part
characteristics.text: Caenorhabditis elegans
characteristics.characteristic: scientific_name
characteristics.text: HA1981
characteristics.characteristic: strain
characteristics.text: WT_3
characteristics.characteristic: title