characteristics.text: 2025-11-15
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: GELIFES, University of Groningen
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS27106565
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-15812:T0_WT_OLD-2
characteristics.characteristic: Submitter Id
characteristics.text: 10 leaves
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: thale cress
characteristics.characteristic: common name
characteristics.text: Arabidopsis thaliana
characteristics.characteristic: cultivar
characteristics.text: Protocols: leaf blades of leaf 3 (old leaf) and leaf 7 (young leaf) were harvested before submergence, after 2 and 4 days of submergence, and after 1, 3, 6, and 24 hours of recovery (Figure 1). 8-16 leaf blades were pooled together per biological replicate. For the Col-0 and bzip28/60 RNA-seq, Arabidopsis plants were harvested before submergence, after 1 and 4 days of submergence, and after 3 hours of recovery in the light. Old (leaves 1-5) and young (leaves 6-10 and the meristem) halves of the rosettes were harvested separately. NA Pots with seeds were germinated and grown in a growth chamber (20°C, 200 μmol m‐2 s‐1 photosynthetically active radiation (PAR), a 9-hour photoperiod from 8:00 to 17:00, and 70% relative humidity). Pots were thinned when seedlings were at 2-leaf stage (with 2 true leaves and 2 cotyledons), so there was only one seedling per pot. Plants were grown to the 10-leaf stage before they were used for experiments.  Flooding treatments were as described previously (Rankenberg et al. 2024, https://doi.org/10.1016/j.xplc.2024.100848) NA RNAseq experiments, RNA was isolated using the RNeasy Plant Mini Kit (Qiagen), residual genomic DNA was digested using on-column DNase-I digestion (Ambion).  TruSeq stranded mRNA
characteristics.characteristic: description
characteristics.text: 10 leaves
characteristics.characteristic: developmental stage
characteristics.text: Col-0
characteristics.characteristic: genotype
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Arabidopsis thaliana
characteristics.characteristic: organism
characteristics.text: Arabidopsis thaliana
characteristics.characteristic: organism
characteristics.text: old leaf
characteristics.characteristic: organism part
characteristics.text: Arabidopsis thaliana
characteristics.characteristic: scientific_name
characteristics.text: control
characteristics.characteristic: stimulus
characteristics.text: T0_WT_OLD-2
characteristics.characteristic: title