characteristics.text: 2025-11-18
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: s58141092@gs.ncku.edu.tw
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS27189143
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-16002:TvBb3
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: Protocols: T. vaginalis co-cultured with B. breve or T. vaginalis alone (negative control) were transferred into centrifuge tubes and centrifuged at 2500 rpm for 5 minutes. The supernatant was removed, and TRIzol reagent was added and mixed thoroughly. Co-culture experiments were conducted to investigate the direct interactions between T. vaginalis and B. breve. Mid-log phase bacteria were standardized to 10⁸ CFU, and parasites were prepared at 10⁷ cells. Both organisms were combined in serum-free YI-S and incubated anaerobically for four hours.  Total RNA was extracted using the Quick-RNA MiniPrep Kit (Zymo Research, Cat. No. R2052) according to the manufacturer’s instructions. Briefly, cells were lysed in RNA Lysis Buffer, and the lysates were passed through spin columns for RNA binding. After on-column DNase I digestion to remove genomic DNA, the columns were washed and RNA was eluted in RNase-free water. RNA concentration and purity were determined using a NanoDrop spectrophotometer, and RNA integrity was verified with an Agilent Bioanalyzer. RNA-seq libraries were constructed using the Illumina Poly(A) Capture (Cat. No. 20777928), cDNA Synthesis (Cat. No. 20761354), and RNA Prep, Ligation (Cat. No. 20778789) kits according to the manufacturer’s protocols.
characteristics.characteristic: description
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Trichomonas vaginalis
characteristics.characteristic: organism
characteristics.text: Trichomonas vaginalis
characteristics.characteristic: organism
characteristics.text: whole organism
characteristics.characteristic: organism part
characteristics.text: Trichomonas vaginalis
characteristics.characteristic: scientific_name
characteristics.text: co-culture with Bifidobacterium breve
characteristics.characteristic: stimulus
characteristics.text: ATCC 30236, ATCC 15700
characteristics.characteristic: strain
characteristics.text: TvBb3
characteristics.characteristic: title