characteristics.text: SAMN40628765
characteristics.characteristic: External Id
characteristics.text: University of Trieste
characteristics.characteristic: INSDC center name
characteristics.text: 2025-06-30T00:53:23.423Z
characteristics.characteristic: INSDC first public
characteristics.text: 2025-06-30T00:53:23.423Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS20865049
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Model organism or animal
characteristics.characteristic: NCBI submission model
characteristics.text: Model.organism.animal.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS20865049
characteristics.characteristic: SRA accession
characteristics.text: not applicable
characteristics.characteristic: age
characteristics.text: Lab Marta Giacomello_Via U. Bassi, 58/B - 35121 Padova
characteristics.characteristic: biomaterial_provider
characteristics.text: Nonagouti (a/a), otherwise wild-type
characteristics.characteristic: breed
characteristics.text: melan-a
characteristics.characteristic: cell_line
characteristics.text: Melanocytes
characteristics.characteristic: cell_type
characteristics.text: Ana Paula Magalhaes Rebelo_University of Padova
characteristics.characteristic: collected_by
characteristics.text: 2022-10-30
characteristics.characteristic: collection_date
characteristics.text: 15
characteristics.characteristic: counter_column
characteristics.text: not applicable
characteristics.characteristic: cultivar
characteristics.text: Cells were electroporated with 40nM siRNA DRP1 and kept in culture for 66h before RNA extractions.
characteristics.characteristic: description
characteristics.text: not applicable
characteristics.characteristic: dev_stage
characteristics.text: not applicable
characteristics.characteristic: ecotype
characteristics.text: Pigmented
characteristics.characteristic: genotype
characteristics.text: Italy: Padova
characteristics.characteristic: geo_loc_name
characteristics.text: Cells were maintained in RPMI-1640 medium supplemented with 10% FBS, 100 U/mL penicillin, 100 g/mL streptomycin, and 200 nM PMA. Cells were kept at 70% confluency between experiments at 37C in a 10% CO2 incubator.
characteristics.characteristic: growth_protocol
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: Cell culture
characteristics.characteristic: sample_type
characteristics.text: not applicable
characteristics.characteristic: sex
characteristics.text: C57BL/6J
characteristics.characteristic: strain
characteristics.text: Cell line was obtained from epidermal melanoblasts from embryos of inbred C57BL mice. Melan-a melanocytes are normal, immortalized murine melanocyte cells derived from C57BL/6J mice. Melan-a melanocytes were obtained from the Wellcome Trust Functional Genomics Cell Bank, St. Georges Hospital, London, UK
characteristics.characteristic: tissue
characteristics.text: melan-a_ SIRNA DRP1, R3
characteristics.characteristic: title