characteristics.text: SAMN41390278
characteristics.characteristic: External Id
characteristics.text: Istituto Italiano di Tecnologia
characteristics.characteristic: INSDC center name
characteristics.text: 2025-06-19T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2025-06-19T14:38:01.938Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS21296649
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Model organism or animal
characteristics.characteristic: NCBI submission model
characteristics.text: Model.organism.animal.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS21296649
characteristics.characteristic: SRA accession
characteristics.text: 129/Ola
characteristics.characteristic: breed
characteristics.text: E14Tg2a
characteristics.characteristic: cell_line
characteristics.text: Mettl9 KO
characteristics.characteristic: cell_type
characteristics.text: not applicable
characteristics.characteristic: collection_date
characteristics.text: 5 DIV
characteristics.characteristic: dev_stage
characteristics.text: not applicable
characteristics.characteristic: geo_loc_name
characteristics.text: mESCs were cultured in Serum/LIF (Dulbecco′s Modified Eagle′s Medium - high glucose), 15% Fetal bovine serum, 2 mM L-Glutamine, 1X MEM Non-Essential Amino Acids Solution, 1 mM Sodium Pyruvate, 50 μM 2-Mercaptoethanol, 5 ng/ml (1000U/ml) LIF. mESCs were detached with Trypsin-EDTA 1X or StemPro™ Accutase™ Cell Dissociation Reagent and seeded on Gelatin-coated plates (EmbryoMax® 0.1% Gelatin Solution). For neural differentiation, mESC were cultured in 2i/LIF (DMEM/F-12, no glutamine, 1X B-27™ Supplement, minus vitamin A, 1X N-2 Supplement, 0.1X MEM Non-Essential Amino Acids Solution, 2 mM L-Glutamine, 1 mM Sodium Pyruvate, 50 μM 2-Mercaptoethanol, 1 μM PD0325901- MEK inhibitor , 3 μM CHIR99021 - GSK3 inhibitor, for 1 day (DIV-1 > DIV0). After 24 hrs (DIV0), 2i/LIF media was replaced by WiBi media (DMEM/F-12, no glutamine, 1X B-27™ Supplement (50X), minus vitamin A #12587010, 1X N-2 Supplement (100X) # 17502048, 0.1X MEM Non-Essential Amino Acids Solution, 2 mM L-Glutamine, 1 mM Sodium Pyruvate, 50 μM 2-Mercaptoethanol, 2.5 μM 54AH-Wnt pathway inhibitor, 0.2 μM LDN193189 hydrochloride, which was provided fresh daily until DIV10 (endpoint). From DIV5 until DIV10, WiBi media was supplemented with 0.1 μM SAG dihydrochloride to induce a basal telencephalic fate. During neural differentiation (from DIV3 onwards), cells were cultured on Poly-L-ornithine and Laminin coated plates, with 16 μg/ml Poly-L-ornithine hydrobromide diluted in H20 and 3.2 μg/ml Laminin Mouse Protein diluted in PBS. All cells were cultured at 37 °C with 5% CO2.
characteristics.characteristic: growth_protocol
characteristics.text: DIV5_KO90_KO_1
characteristics.characteristic: isolate
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: blastocyst
characteristics.characteristic: tissue
characteristics.text: Model organism or animal sample from Mus musculus
characteristics.characteristic: title