characteristics.text: 2025-11-19
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: MHH
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS27064795
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-15718:S02
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: CD19 CAR T cell
characteristics.characteristic: cell type
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: CD19-CAR-T cells were generated from human primary CD3+ T cells of three donors in presence or absence of vitamin C. Viable CD3+ T cells were sorted after 24h of exposure to CD19+ B-LCLs.  S01: CD19 CAR T cells pre exposure to CD19+ B-LCLs. HTO capture antibodies were used to allow discrimination between CD19 CAR T cells generated in presence or absence of vitamin C, obtained in three independent experiments (donors). HTO1: donor 1, 0 µM vitamin C HTO2: donor 1, 50 µM vitamin C HTO3: donor 2, 0 µM vitamin C HTO4: donor 2, 50 µM vitamin C HTO5: donor 3, 0 µM vitamin C HTO6: donor 3, 50 µM vitamin C  S02: CD19 CAR T cells post exposure to CD19+ B-LCLs. HTO capture antibodies were used to allow discrimination between CD19 CAR T cells generated in presence or absence of vitamin C, obtained in three independent experiments (donors). HTO1: donor 1, 0 µM vitamin C HTO2: donor 1, 50 µM vitamin C HTO3: donor 2, 0 µM vitamin C HTO4: donor 2, 50 µM vitamin C HTO5: donor 3, 0 µM vitamin C HTO6: donor 3, 50 µM vitamin C Sorted cells were subjected to the 10x pipeline. More details see \"nucleid acid library construction protocol\". Library preparation for single cell mRNA-Seq analysis was performed according to the Chromium NextGEM Single Cell 5ʹ Reagent Kits v2 CellSurfaceProtein User Guide (Manual Part Number CG000330 Rev E; 10x Genomics). According to the protocol a given excess (100%) of cells was loaded to the 10x controller. Fragment length distribution of generated libraries was monitored using ‘Bioanalyzer High Sensitivity DNA Assay’ (5067-4626; Agilent Technologies). Quantification of libraries was performed by use of the ‘Qubit® dsDNA HS Assay Kit’ (Q32854; ThermoFisher Scientific).
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: post exposure CD19+ B-LCLs
characteristics.characteristic: growth condition
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: S02
characteristics.characteristic: title