characteristics.text: 2025-11-20
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: Bioinformatics Core Facility Faculty  of  Biology,  Medicine  &  Health The University of Manchester
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS22467187
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-14646:Sham-irradiated
characteristics.characteristic: Submitter Id
characteristics.text: 22
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: heart cell
characteristics.characteristic: cell_type
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Ten weeks post RT, one control mouse, one irradiated at the base and one at the apex were killed by a regulated procedure. Hearts were immediately extracted, washed in Phosphate buffered saline (PBS) and processed to be embedded in wax.According to 10x protocol for Visium, 5 micrometer sections were collected. Female C57BL/6J aged 12 weeks (16-20gr; Envigo, UK) were housed in standard housing conditions for laboratory animals (12-hour light-dark cycle, controlled temperature of 19–22°C and humidity of 40–65%) and provided with a standard diet and water ad libitum.Animals were anaesthetised with 2% isoflurane and were irradiated with X-rays (220 kV,13mA) at a dose rate of 2.67 Gy/min on a small-animal radiation research platform (SARRP; Xstrahl Life Sciences, UK). Briefly, cone beam CT (60kV, 0.7mA) collected 360 projections over 1 minute and it was used to reconstruct the 3D image of the whole mouse to identify the heart. Muriplan software was adopted to contour the region of interest, either the base or the apex of the heart to deliver the radiation. A total dose of 16Gy was delivered to the specific substructure of the heart using a 3 × 9 mm collimator. Used 10X sample collection protocol, detailed in  CG000409-10x Genomics userguide Used 10X sample collection protocol, CG000409-10x Genomics
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: heart
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.characteristic: sex
characteristics.text: C57BL/6
characteristics.characteristic: strain
characteristics.text: Sham-irradiated
characteristics.characteristic: title