characteristics.text: 2025-11-20
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: Stockholm university
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS27386126
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-16210:CUT_Tag_CKO_rep2
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: spermatogonium
characteristics.characteristic: cell type
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: FACS-purified spermatogonia (SpG) were isolated from adult mouse testes. Cells were collected and counted prior to CUT&Tag library preparation. Following tagmentation and reverse crosslinking, DNA was purified using AMPure XP beads (Beckman Coulter, A63880) according to the manufacturer’s instructions, and eluted in 22 µl Tris-HCl (pH 8.5). CUT&Tag libraries were prepared as described by Henikoff et al. (2020) (DOI: 10.17504/protocols.io.x54v9mkmzg3e/v5). Briefly, ~80,000 cells were bound to Concanavalin A-coated magnetic beads and incubated sequentially with primary and secondary antibodies, followed by pAG-Tn5 tagmentation at 55 °C for 1 h. DNA was released with SDS–Proteinase K treatment, PCR-amplified using NEBNext 2× PCR master mix (11 cycles, 98 °C 10 s / 60 °C 10 s), and purified with AMPure XP beads. Final libraries were quantified and assessed for quality before sequencing.
characteristics.characteristic: description
characteristics.text: CKO
characteristics.characteristic: genotype
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: testis
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: C57BL/6J
characteristics.characteristic: strain
characteristics.text: CUT_Tag_CKO_rep2
characteristics.characteristic: title