characteristics.text: SAMN47181590
characteristics.characteristic: External Id
characteristics.text: Helmholtz Centre for Environmental Research (UFZ)
characteristics.characteristic: INSDC center name
characteristics.text: 2025-03-31T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2025-09-26T06:54:40.085Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS24227583
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Metagenome or environmental
characteristics.characteristic: NCBI submission model
characteristics.text: Metagenome.environmental.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS24227583
characteristics.characteristic: SRA accession
characteristics.text: 2022-09
characteristics.characteristic: collection_date
characteristics.text: Pass 1, methanogenic, 50µM_Sitagliptin_3
characteristics.characteristic: description
characteristics.text: Germany: River Erpe
characteristics.characteristic: geo_loc_name
characteristics.text: not applicable
characteristics.characteristic: host
characteristics.text: enrichment from river sediment
characteristics.characteristic: isolation_source
characteristics.text: 52.4786 N 13.6351 E
characteristics.characteristic: lat_lon
characteristics.text: freshwater sediment metagenome
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_556182
characteristics.characteristic: organism
characteristics.text: anaerobe
characteristics.characteristic: rel_to_oxygen
characteristics.text: DNA was extracted from cell pellets from an enrichment culture after 287 days of incubation using the DNeasy PowerSoil Pro kit (Qiagen). Extracted DNA was sent to BGI for sequencing. The V3-V4 hypervariable region was amplified using the 338F (ACTCCTACGGGAGGCAGCAG) and 806R (GGACTACHVGGGTWTCTAAT) primers. Sequencing on the DNBSEQ-G400 platform in the paired-end mode with a read length of 300 bp per read.
characteristics.characteristic: samp_mat_process
characteristics.text: 1 ml
characteristics.characteristic: samp_size
characteristics.text: 9
characteristics.characteristic: sample_ID
characteristics.text: Pass1 SSM3
characteristics.characteristic: title