characteristics.text: 2025-11-21
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: 1. Institut Pasteur, Université Paris Cité, Plate-forme Technologique Biomics, F-75015 Paris, France 2. Institut Pasteur, Université Paris Cité, Bioinformatics and Biostatistics Hub, F-75015 Paris, France
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS27360738
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-16208:Norm2
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: stem cell derived cell line
characteristics.characteristic: cell line
characteristics.text: stem cell
characteristics.characteristic: cell type
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Seven days after cardiotoxin-induced muscle injury, Tibialis Anterior (TA) muscles from Tg:Pax7-nGFP mice exposed to normoxic (20%) or hypoxic (10%) environment were dissected, minced and incubated with a mix of 2.4U/ml Dispase II (Roche, #04942078001) and 100μg/ml collagenase A (Roche, #11088793001) in 4.5g/L glucose 1x DMEM (GibcoTM; #11995) at 37 °C on a shaking plate for 2 h. The muscle suspension was filtrated through 100-μm and 70-μm cell strainers and then spun at 50g for 10min at 4°C to remove large tissue fragments. Then, the supernatant was collected, filtered through a 40-μm cell strainer and washed by centrifugation at 700g for 10 min at 4°C. The final pellet has been resuspended in cold 4.5g/L glucose 1xDMEM supplemented with 0.2% bovine serum albumin (BSA, Sigma-Aldrich). The muscle cell suspension was used to sort 140 000 GFP+ MuSCs by fluorescence-activated cell sorting (FACS)-sorting using the GFP reporter (n=4 per atmospheric condition). Total RNA was extracted from GFP+ myogenic cells isolated from injured TA using RNAqueous™-Micro Kit RNA Isolation ™ (Invitrogen™, #AM1931). Library preparation and sequencing was performed by the Biomics platform at the Institut Pasteur. RNA preparation was used to build libraries using an Illumina Stranded mRNA library Preparation Kit (Illumina, USA) following the manufacturer’s protocol. Quality control was performed on an Agilent BioAnalyzer
characteristics.characteristic: description
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: normoxia
characteristics.characteristic: growth condition
characteristics.text: acute muscle injury by cardiotoxin intramuscular injection
characteristics.characteristic: injury
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: skeletal muscle
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: Norm2
characteristics.characteristic: title