characteristics.text: SAMN46353212
characteristics.characteristic: External Id
characteristics.text: Donghua university
characteristics.characteristic: INSDC center name
characteristics.text: 2025-10-10T00:50:39.563Z
characteristics.characteristic: INSDC first public
characteristics.text: 2025-10-10T00:50:39.563Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS23850590
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: MIGS.eu
characteristics.characteristic: NCBI submission model
characteristics.text: MIGS/MIMS/MIMARKS.soil
characteristics.characteristic: NCBI submission model
characteristics.text: MIGS.eu.soil.6.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS23850590
characteristics.characteristic: SRA accession
characteristics.text: 2023-12-10
characteristics.characteristic: collection_date
characteristics.text: M.N. Khalil
characteristics.characteristic: cultivar
characteristics.text: 30 cm
characteristics.characteristic: depth
characteristics.text: Keywords: GSC:MIxS;MIGS:6.0
characteristics.characteristic: description
characteristics.text: "Genomic DNA was extracted using the Cetyltrimethyl Ammonium Bromide (CTAB) method with minor modifications to improve yield and purity. The DNA concentration, quality, and integrity were assessed using a Qubit Fluorometer (Invitrogen, USA) and a NanoDrop Spectrophotometer (Thermo Scientific, USA). Sequencing libraries were prepared using the Illumina TruSeq DNA Sample Preparation Kit and the Template Prep Kit (Pacific Biosciences, USA), following the respective manufacturers' protocols. Genomic DNA was fragmented using a Covaris system to generate fragments approximately 300-350 bp in size. The DNA was subjected to double-end repair, an 'A' base was added to the 3' ends, and sequencing adapters were ligated. Adapter-ligated fragments were enriched through PCR amplification and purified using magnetic beads. Library quality and fragment size distribution were verified using an Agilent Bioanalyzer 2100 and quantified using the Quant-iT PicoGreen dsDNA Assay Kit.
characteristics.characteristic: design_description
characteristics.text: 10 cm
characteristics.characteristic: elev
characteristics.text: Agricultural Soil
characteristics.characteristic: env_broad_scale
characteristics.text: Soil collected from Donghua University
characteristics.characteristic: env_local_scale
characteristics.text: Rice straw (used in pretreatment)
characteristics.characteristic: env_medium
characteristics.text: f2_PE400_R1.fastq.gz
characteristics.characteristic: filename
characteristics.text: f2_PE400_R2.fastq.gz
characteristics.characteristic: filename2
characteristics.text: fastq
characteristics.characteristic: filetype
characteristics.text: China: Donghua University, Shanghai
characteristics.characteristic: geo_loc_name
characteristics.text: Illumina NovaSeq 6000
characteristics.characteristic: instrument_model
characteristics.text: Isolation Source: Soil sample collected from the Donghua University campus. The organism was isolated from soil associated with agricultural activities, specifically for its potential in the pretreatment of rice straw. Growth Conditions: Penicillium chrysogenum was grown on Potato Dextrose Agar (PDA) at 25C for 5 days. The pH of the medium was adjusted to neutral. The isolate was subsequently maintained under laboratory conditions for experimental purposes.
characteristics.characteristic: isol_growth_condt
characteristics.text: 31.2323 N 121.4031 E
characteristics.characteristic: lat_lon
characteristics.text: SampleA_TruSeq_PacBio(YF20223649)
characteristics.characteristic: library_id
characteristics.text: paired
characteristics.characteristic: library_layout
characteristics.text: RANDOM PCR
characteristics.characteristic: library_selection
characteristics.text: GENOMIC
characteristics.characteristic: library_source
characteristics.text: WGS
characteristics.characteristic: library_strategy
characteristics.text: Penicillium chrysogenum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_5076
characteristics.characteristic: organism
characteristics.text: ILLUMINA
characteristics.characteristic: platform
characteristics.text: F2
characteristics.characteristic: title
characteristics.text: F2
characteristics.characteristic: title