characteristics.text: SAMN46281603
characteristics.characteristic: External Id
characteristics.text: Skolkovo Institute of Science and Technology
characteristics.characteristic: INSDC center name
characteristics.text: 2025-10-01T00:54:49.300Z
characteristics.characteristic: INSDC first public
characteristics.text: 2025-10-01T00:54:49.300Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS23811605
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: MIMS.me
characteristics.characteristic: NCBI submission model
characteristics.text: MIGS/MIMS/MIMARKS.host-associated
characteristics.characteristic: NCBI submission model
characteristics.text: MIMS.me.host-associated.6.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS23811605
characteristics.characteristic: SRA accession
characteristics.text: 2022-09-17
characteristics.characteristic: collection_date
characteristics.text: 10 m
characteristics.characteristic: depth
characteristics.text: Amplicons of V3-V4 region of 16S rRNA gene from H. panicea metagenome, 2022, White Sea WSBS
characteristics.characteristic: description
characteristics.text: Marine benthic biome [ENVO_01000024]
characteristics.characteristic: env_broad_scale
characteristics.text: Marine neritic benthic zone biome [ENVO_01000025]
characteristics.characteristic: env_local_scale
characteristics.text: Sea water [ENVO_00002149]
characteristics.characteristic: env_medium
characteristics.text: Russia: N. Pertsov White Sea Biological Station in the Kandalaksha Bay of the White sea
characteristics.characteristic: geo_loc_name
characteristics.text: Halichondria panicea
characteristics.characteristic: host
characteristics.text: Breadcrumb sponge
characteristics.characteristic: host_common_name
characteristics.text: Adult attached sponge
characteristics.characteristic: host_life_stage
characteristics.text: 4940
characteristics.characteristic: host_subject_id
characteristics.text: 66.5527 N 33.1033 E
characteristics.characteristic: lat_lon
characteristics.text: sponge metagenome
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_1163772
characteristics.characteristic: organism
characteristics.text: SCUBA diving
characteristics.characteristic: samp_collect_device
characteristics.text: A fragment of sponge tissue was diced and fragmented by razor and forceps in 25 mL of sterile marine water. The obtained suspension was centrifuged at 1000 rpm for 3 min. The supernatant was collected and centrifuged further at 4700 rpm for 10 min. Resulatnt pellet was used for DNA extraction. A V3-V4 region of 16S rRNA gene was amplified using standard degenerate primers fused with sequencing adapters (Illumina). PCR Forward Primer = CCTACGGGNGGCWGCAG, PCR Reverse Primer = GACTACHVGGGTATCTAATCC, adapter sequences are not shown.
characteristics.characteristic: samp_mat_process
characteristics.text: A fragment of an individual sponge, ~50 g
characteristics.characteristic: samp_size
characteristics.text: 1 cm3
characteristics.characteristic: samp_vol_we_dna_ext
characteristics.text: 5 C
characteristics.characteristic: temp
characteristics.text: Amplicons of V3-V4 region of 16S rRNA gene from H. panicea metagenome, 2022, White Sea WSBS
characteristics.characteristic: title