characteristics.text: 2025-11-21
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: UMass Chan Medical School
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS27376725
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-16144:AB_4
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: CD4-positive, alpha-beta T cell
characteristics.characteristic: cell type
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Epitope-specific CD4 T cells were isolated from PBMC cultured in vitro in presence of epitope peptides (10 ug/mL) for ~12-15 days, with IL2 addition to the medium at days 4 and 8. Isolation was performed using DRB1*03:01 tetramers carrying the epitope peptides and flow cytometry-based cell sorting.  Total RNA was extracted from tetramer-sorted cells using RNeasy kit (Qiagen). Libraries were prepared from 250 ng of RNA with RQN>7.2, using the Illumina TruSeq v2 platform and following manufacturer's instructions.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: Herpesviridae infectious disease
characteristics.characteristic: disease
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: d351
characteristics.characteristic: individual
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: blood
characteristics.characteristic: organism part
characteristics.text: Unexpanded
characteristics.characteristic: response to treatment
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: Unstimulated
characteristics.characteristic: stimulus
characteristics.text: AB_4
characteristics.characteristic: title