characteristics.text: 2025-11-21
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: Helmholtz
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS23086511
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-14806:Cq_control_rep1
characteristics.characteristic: Submitter Id
characteristics.text: 7
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: quinoa
characteristics.characteristic: common name
characteristics.text: 5020
characteristics.characteristic: cultivar
characteristics.text: Protocols: Guard cells were enriched using the blender method (Bauer et al., 2013). Chenopodium quinoa (cv.5020) plants were cultivated in a greenhouse (at 22°C / 20°C day / night under a 16h light regime at 300 µmol m-2 s-1 white light) using common potting soil. NaCl- treatment was started after 2 weeks of growth. The NaCl concentration in the irrigation water started at 25 mM NaCl and was increased stepwise by doubling the concentration every second day up to 200 mM or 500 mM. After 5 weeks of salt treatment plants were used for experiments. For ABA stimulation of guard cells, ABA (50 µM ± ABA, Sigma) was fed to the stomata via the petiole of detached leaves, as the dense epidermal bladder growth prevented the usual spray treatment. Guard cells were enriched using the blender method (Bauer et al., 2013) and DNase I treated. Library preparation and RNAseq were carried out as described in the Illumina TruSeq Stranded mRNA Sample Preparation Guide, the Illumina NextSeq 500 System Guide (Illumina, Inc., San Diego, CA, USA), and the KAPA Library Quantification Kit - Illumina/ABI Prism User Guide (Kapa Biosystems, Inc., Woburn, MA, USA).
characteristics.characteristic: description
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Chenopodium quinoa
characteristics.characteristic: organism
characteristics.text: Chenopodium quinoa
characteristics.characteristic: organism
characteristics.text: stomatal complex
characteristics.characteristic: organism part
characteristics.text: Chenopodium quinoa
characteristics.characteristic: scientific_name
characteristics.text: control
characteristics.characteristic: stimulus
characteristics.text: Cq_control_rep1
characteristics.characteristic: title