characteristics.text: SAMN46543281
characteristics.characteristic: External Id
characteristics.text: Baylor College of Medicine
characteristics.characteristic: INSDC center name
characteristics.text: 2025-07-01T00:52:21.877Z
characteristics.characteristic: INSDC first public
characteristics.text: 2025-07-01T00:52:21.877Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS23977380
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Microbe, viral or environmental
characteristics.characteristic: NCBI submission model
characteristics.text: Microbe.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS23977380
characteristics.characteristic: SRA accession
characteristics.text: not collected
characteristics.characteristic: collection_date
characteristics.text: CRISPRI-Seq of the indicated genotype done on LB media to idenitify synthetic genetic interactions. Library preparation is a set of nested PCRs to amplify sgRNA plasmid pool sgRNA regions. Sequencing is custom on a NextSeq 550 platform using HPLC-purified R1,I1, and I2 primers and a sequencing cycle allocation of: Read1 = 2 dark cycles + 20 cycles; Index1 = 8 cycles; Index2 = 8 cycles; Read2 = 0 cycles. Pre-selection. Replicate 3.
characteristics.characteristic: description
characteristics.text: ptet-dCas9, rep
characteristics.characteristic: genotype
characteristics.text: not collected
characteristics.characteristic: geo_loc_name
characteristics.text: not applicable
characteristics.characteristic: host
characteristics.text: REP_T0_REP3
characteristics.characteristic: isolate
characteristics.text: not applicable
characteristics.characteristic: isolation_source
characteristics.text: Escherichia coli
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_562
characteristics.characteristic: organism
characteristics.text: Cell Culture
characteristics.characteristic: sample_type
characteristics.text: K-12 str. MG1655
characteristics.characteristic: strain
characteristics.text: Microbe sample from Escherichia coli
characteristics.characteristic: title