characteristics.text: SAMN46873560
characteristics.characteristic: External Id
characteristics.text: Ocean University of China
characteristics.characteristic: INSDC center name
characteristics.text: 2025-10-09T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2025-10-09T22:16:01.800Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS24114863
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Model organism or animal
characteristics.characteristic: NCBI submission model
characteristics.text: Model.organism.animal.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS24114863
characteristics.characteristic: SRA accession
characteristics.text: 2024-11-05
characteristics.characteristic: collection_date
characteristics.text: A total of five million micronuclei (MICs) were purified from RIB1 knock out Tetrahymena meiotic cells that expressing endogenous Rib1-CTD-MicNLS2, then the MICs were used for the Cleavage Under Targets and Tagmentation (CUT&Tag) experiment for the identification of Rib1-CTD binding regions in the meiotic MIC. CUT&Tag was conducted using BioMag Plus Concanavalin A beads (Bangs Laboratories, Fishers, IN), a commercial Hyperactive Universal CUT&Tag Assay Kit for Illumina (Vazyme, Nanjing, China), and an anti-HA rabbit monoclonal antibody (Clone C29F4, Cell Signaling Technology). CUT&Tag libraries were generated using indexed primers and the KAPA HiFi HotStart Real-Time Library Amplification Kit (Roche) and subsequently sequenced on a NovaSeq 6001 platform (Illumina, San Diego, CA).
characteristics.characteristic: description
characteristics.text: C3
characteristics.characteristic: dev_stage
characteristics.text: China
characteristics.characteristic: geo_loc_name
characteristics.text: Tetrahymena thermophila
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_5911
characteristics.characteristic: organism
characteristics.text: pooled male and female
characteristics.characteristic: sex
characteristics.text: rib1ko
characteristics.characteristic: strain
characteristics.text: single cell culture
characteristics.characteristic: tissue
characteristics.text: Rib1-CTD-NLS C3 CUTtag
characteristics.characteristic: title