characteristics.text: 2025-11-22
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: Department of Artificial Intelligence Medicine, Chiba University Graduate School of Medicine
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS20757218
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-14315:P17CtrlRetina
characteristics.characteristic: Submitter Id
characteristics.text: 17
characteristics.unit: day
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: blood vessel endothelial cell
characteristics.characteristic: cell_type
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: In the oxygen-induced retinopathy (OIR) model, wild-type C57BL/6J mice were exposed to 75% oxygen (hyperoxia) from P7 to P12 and returned to normal oxygen (normoxia) at P12. Retinal tissues and optic nerves were dissected from OIR and normoxic (control) mice at P12, P17, and P25. Tissues were digested with dispase I, type I collagenase, and type II collagenase. Cells were stained with anti-mouse CD31 and CD45 antibodies. Dead cells were stained with propidium iodide and excluded. CD31+CD45- vascular endothelial cells (ECs) were isolated by fluorescence-activated cell sorting (FACS).  ECs were then loaded into the 10X Chromium Single Cell 3′ Kit (v3) to obtain 5,000 cells per well, and library preparation was performed according to the manufacturer's protocol.  ECs were then loaded into the 10X Chromium Single Cell 3′ Kit (v3) to obtain 5,000 cells per well, and library preparation was performed according to the manufacturer's protocol.
characteristics.characteristic: description
characteristics.text: infant
characteristics.characteristic: developmental stage
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: 10 individuals
characteristics.characteristic: individual
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: retina
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: mixed
characteristics.characteristic: sex
characteristics.text: normoxia
characteristics.characteristic: stimulus
characteristics.text: C57BL/6J
characteristics.characteristic: strain
characteristics.text: P17CtrlRetina
characteristics.characteristic: title