characteristics.text: 2025-11-22
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: SAMEA112152907
characteristics.characteristic: External Id
characteristics.text: KING'S COLLEGE LONDON
characteristics.characteristic: INSDC center name
characteristics.text: 2022-11-22T17:08:23Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS14262772
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-12382:3-CON3
characteristics.characteristic: Submitter Id
characteristics.text: 6
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Mouse islets were isolated between 10:00 and 11:00 hours. Pancreata were inflated with 1mg/mL collagenase solution (Sigma-Aldrich, Poole, U.K.) followed by density gradient separation (Histopaque-1077; Sigma-Aldrich). Isolated islets were maintained in supplemented RPMI at 37 °C and 5% CO2 in a humidified atmosphere for up to 24 hours prior to experimental treatment. Isolated mouse islets were subjected for 24 h to (A) no treatment, (B) a cytokine cocktail (IL-1β, TNFα and IFNγ; 1 ng/ml) or (C) FK866 (10 nM) and cytokine cocktail. Total RNA was extracted from ∼150 islets per mouse, according to the manufacturer's instructions (RNeasy Mini Kit, Qiagen, UK) 100ng total RNA from each sample was used as input to generate library for RNAseq. RNAseq libraries were prepared using NEBNext Ultra II Directional RNA Library Prep kit (New England Biolabs, Hertfordshire, UK. Cat.no: E7760) following manufacturer’s recommendations.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: normal
characteristics.characteristic: disease
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: islet of Langerhans
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: CD1
characteristics.characteristic: strain
characteristics.text: 3-CON3
characteristics.characteristic: title