characteristics.text: 2025-11-23
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: Department of Biochemistry & Biophysics, University of California, San Francisco, San Francisco, California, USA.
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS27226715
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-16091:MDA_chr17_rep2
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: MDA-MB-231
characteristics.characteristic: cell line
characteristics.text: breast adenocarcinoma cell line
characteristics.characteristic: cell type
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: All cells were cultured in a 37°C 5% CO2 humidified incubator. MDA-MB-231 cells were cultured in Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10% FBS, glucose (2 g/L), L-glutamine (2 mM), 25 mM HEPES, penicillin (100 units/mL), streptomycin (100 μg/mL) and amphotericin B (1 μg/mL) (Gibco). HCC1806-LM2 (HCC-LM2) cells were grown in Roswell Park Memorial Institute-1640 medium supplemented with 10% FCS, l-glutamine (2 mM), sodium pyruvate (1 mM), penicillin (100 U ml−1), streptomycin (100 μg ml−1) and amphotericin (1 μg ml−1). RNA was extracted extracted using the Zymo Research Quick-RNA Microprep Kit (cat#R1051), following manufacturer's instructions. mRNA-seq libraries were constructed from 100-200 ng RNA using the QuantSeq 3’ mRNA-Seq Library Prep Kit FWD according to the manufacturer’s instructions (Lexogen, Cat. #015).
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: Breast adenocarcinoma
characteristics.characteristic: disease
characteristics.text: Transduced with oncRNA.ch17.67
characteristics.characteristic: genetic modification
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: breast
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: MDA_chr17_rep2
characteristics.characteristic: title