characteristics.text: 2025-11-23
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: Japanese Foundation for Cancer Research
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS27402933
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-16244:Sample 7
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: HCT215-1T
characteristics.characteristic: cell line
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Patient derived organoids were treated with 100 nM AMG510 for 24 h. Total RNA was extracted using an RNeasy mini kit (Qiagen) and quantified using 2100 Expert Bioanalyzer software (Agilent). RNA-seq analysis was outsourced to Macrogen Japan Corp. (Tokyo, Japan). Following the manufacturer’s instructions, total RNA with an RIN>7 was used to generate a barcode-labeled library using an Illumina TruSeq Stranded mRNA LT Sample Prep Kit (Illumina).
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: colorectal cancer
characteristics.characteristic: disease
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: 1
characteristics.characteristic: replicate
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: Sample 7
characteristics.characteristic: title