characteristics.text: 2025-11-24
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: LIPME
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS23983592
characteristics.characteristic: SRA accession
characteristics.text: Ade_Atha_C1
characteristics.characteristic: Submitter Id
characteristics.text: 5W
characteristics.characteristic: age
characteristics.text: 2023-10-18
characteristics.characteristic: collection date
characteristics.text: thale cress
characteristics.characteristic: common name
characteristics.text: Isolated nuclei of Arabidopsis leaves.
characteristics.characteristic: description
characteristics.text: 5 week old plants
characteristics.characteristic: dev_stage
characteristics.text: Col-0
characteristics.characteristic: ecotype
characteristics.text: RNA
characteristics.characteristic: experimental factor 1
characteristics.text: Non-infected
characteristics.characteristic: experimental factor 2
characteristics.text: Col-0
characteristics.characteristic: genotype
characteristics.text: France
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: Grown in Jiffy pots under controlled conditions at 22°C with a 9-hour light period at an intensity of 120 μmol/m2/s
characteristics.characteristic: growth condition
characteristics.text: control
characteristics.characteristic: infect
characteristics.text: Arabidopsis thaliana
characteristics.characteristic: organism
characteristics.text: leaf
characteristics.characteristic: organism part
characteristics.text: Susceptible
characteristics.characteristic: phenotype
characteristics.text: Arabidopsis thaliana Columbia-0 genotype was grown in Jiffy pots under controlled conditions at 22°C with a 9-hour light period at an intensity of 120 μmol/m2/s. Leaves of five-week-old plants were inoculated with S. sclerotiorum strain 1980 (ATCC18683)  using 0.5-cm-wide plugs of Potato Dextrose Agar (PDA) medium colonized by the fungus. Control samples were also prepared identially but with uncolonised agar plugs. The plugs were placed in the center of the adaxial surface of leaves, and plants were incubated in trays sealed with plastic wrap to maintain 80% relative humidity. Entire leaves were collected individually for nuclei extraction 24 hours, with two independent biological replicates per treatment. One complete leaf per sample was collected and agar plugs were removed. . Leaves were transferred to pre-chilled lysis buffer and chopped with a razor blade, as described in Guillotin et al., 2023 (https://doi.org/10.1038/s41586-023-06053-0). Immediately after 10µm filtration at 1000g centrifugation, the nuclei were stained with propidium iodide and isolated using fluorescense-activated cell sorting (FACS) performed by the Cytek Aurora Cell Sorter form Cytometry TRI Genotoul facility (Toulouse, France). FACS isolated 6,000 nuclei per sample. SnRNA-seq libraries were prepared at the Get-Santé facility (INSERM Rangueil, France) using the Chromium Next GEM Single Cell 3\' Kit v3.1 (10x Genomics reference) and the Chromium Next GEM Chip G Single Cell Kit (10x Genomics), following the manufacturer\'s protocol.
characteristics.characteristic: protocol
characteristics.text: 1
characteristics.characteristic: replicate
characteristics.text: 24H
characteristics.characteristic: sampling time point
characteristics.text: Arabidopsis thaliana
characteristics.characteristic: scientific_name
characteristics.text: 24H
characteristics.characteristic: time
characteristics.text: Arabidopsis leaves
characteristics.characteristic: title