characteristics.text: 2025-11-24
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: LIPME
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS23983851
characteristics.characteristic: SRA accession
characteristics.text: Ade_Atha_AW1000
characteristics.characteristic: Submitter Id
characteristics.text: 5W
characteristics.characteristic: age
characteristics.text: 2023-10-18
characteristics.characteristic: collection date
characteristics.text: thale cress
characteristics.characteristic: common name
characteristics.text: Isolated nuclei of Arabidopsis leaves exposed to sine audio wave at 1000Hz for 15 minutes and extracted directly after treatment.
characteristics.characteristic: description
characteristics.text: 5 week old plants
characteristics.characteristic: dev_stage
characteristics.text: Col-0
characteristics.characteristic: ecotype
characteristics.text: RNA
characteristics.characteristic: experimental factor 1
characteristics.text: Sine wave 1000 Hz
characteristics.characteristic: experimental factor 2
characteristics.text: Col-0
characteristics.characteristic: genotype
characteristics.text: France
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: Grown in Jiffy pots under controlled conditions at 22°C with a 9-hour light period at an intensity of 120 μmol/m2/s
characteristics.characteristic: growth condition
characteristics.text: Arabidopsis thaliana
characteristics.characteristic: organism
characteristics.text: leaf
characteristics.characteristic: organism part
characteristics.text: Arabidopsis thaliana Columbia-0 genotype was grown in Jiffy pots under controlled conditions at 22°C with a 9-hour light period at an intensity of 120 μmol/m2/s. Five week old plants were exposed to an audio sine wave at 1000 Hz for 15 minutes, and leaves were excised immediately after treatment for processing. Leaves were transferred to pre-chilled lysis buffer and chopped with a razor blade, as described in Guillotin et al., 2023 (https://doi.org/10.1038/s41586-023-06053-0). Immediately after 10µm filtration at 1000g centrifugation, the nuclei were stained with propidium iodide and isolated using fluorescense-activated cell sorting (FACS) performed by the Cytek Aurora Cell Sorter form Cytometry TRI Genotoul facility (Toulouse, France). FACS isolated 6,000 nuclei per sample. SnRNA-seq libraries were prepared at the Get-Santé facility (INSERM Rangueil, France) using the Chromium Next GEM Single Cell 3\' Kit v3.1 (10x Genomics reference) and the Chromium Next GEM Chip G Single Cell Kit (10x Genomics), following the manufacturer\'s protocol.
characteristics.characteristic: protocol
characteristics.text: 1
characteristics.characteristic: replicate
characteristics.text: 24H
characteristics.characteristic: sampling time point
characteristics.text: Arabidopsis thaliana
characteristics.characteristic: scientific_name
characteristics.text: Arabidopsis leaves treated with audio 1000Hz
characteristics.characteristic: title