characteristics.text: 2025-11-24
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: LIPME
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS23983852
characteristics.characteristic: SRA accession
characteristics.text: Ade_Atha_V1_SI
characteristics.characteristic: Submitter Id
characteristics.text: 5W
characteristics.characteristic: age
characteristics.text: 2023-10-18
characteristics.characteristic: collection date
characteristics.text: thale cress
characteristics.characteristic: common name
characteristics.text: Isolated nuclei of Arabidopsis leaves exposed to wind at 1.2 m/s for 15 minutes and extracted directly after treatment.
characteristics.characteristic: description
characteristics.text: 5 week old plants
characteristics.characteristic: dev_stage
characteristics.text: Col-0
characteristics.characteristic: ecotype
characteristics.text: RNA
characteristics.characteristic: experimental factor 1
characteristics.text: Wind 1.2 m/s
characteristics.characteristic: experimental factor 2
characteristics.text: Col-0
characteristics.characteristic: genotype
characteristics.text: France
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: Grown in Jiffy pots under controlled conditions at 22°C with a 9-hour light period at an intensity of 120 μmol/m2/s
characteristics.characteristic: growth condition
characteristics.text: Arabidopsis thaliana
characteristics.characteristic: organism
characteristics.text: leaf
characteristics.characteristic: organism part
characteristics.text: Arabidopsis thaliana Columbia-0 genotype was grown in Jiffy pots under controlled conditions at 22°C with a 9-hour light period at an intensity of 120 μmol/m2/s. Five week old plants were exposed to wind from a fan at 1.2 m/s for 15 minutes, and leaves were excised immediately after treatment for processing. Leaves were transferred to pre-chilled lysis buffer and chopped with a razor blade, as described in Guillotin et al., 2023 (https://doi.org/10.1038/s41586-023-06053-0). Immediately after 10µm filtration at 1000g centrifugation, the nuclei were stained with propidium iodide and isolated using fluorescense-activated cell sorting (FACS) performed by the Cytek Aurora Cell Sorter form Cytometry TRI Genotoul facility (Toulouse, France). FACS isolated 6,000 nuclei per sample. SnRNA-seq libraries were prepared at the Get-Santé facility (INSERM Rangueil, France) using the Chromium Next GEM Single Cell 3\' Kit v3.1 (10x Genomics reference) and the Chromium Next GEM Chip G Single Cell Kit (10x Genomics), following the manufacturer\'s protocol.
characteristics.characteristic: protocol
characteristics.text: 1
characteristics.characteristic: replicate
characteristics.text: 24H
characteristics.characteristic: sampling time point
characteristics.text: Arabidopsis thaliana
characteristics.characteristic: scientific_name
characteristics.text: Arabidopsis leaves treated with wind
characteristics.characteristic: title