characteristics.text: 2025-11-25
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: etcenter@126.com
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS27226728
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-16092:T6271
characteristics.characteristic: Submitter Id
characteristics.text: 2
characteristics.unit: year
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: domestic cattle
characteristics.characteristic: common name
characteristics.text: Protocols: The six experimental cattle used in this study were sourced from Shandong Zhaofu Animal Husbandry Technology Co., Ltd. The barns provided adequate lighting and ventilation, with suitable temperature and humidity, and all animals were maintained under consistent feeding conditions. The study protocol was approved by the Qingdao Agricultural University Institutional Animal Care and Use Committee (IACUC) (Approval No. DKY20220805, dated August 5, 2022). Placental tissues were collected postpartum, and their length, width, thickness, and weight were measured. Cotyledon tissues from the same anatomical location were collected and stored in liquid nitrogen at -80°C. calf birth weight, body height, chest circumference, and waistline were recorded. Based on placental weight, the cattle were divided into Group P (≥5.5 kg, n=3) and Group S (<5.5 kg, n=3). After total RNA was extracted by Trizol reagent kit (Invitrogen, Carlsbad, CA,USA), the RNA  molecules in a size range of 18–30nt were enriched by polyacrylamide gel  electrophoresis(PAGE). T Then the 3’ adapters were added and the 36-48nt RNAs were enriched.  The 5’ adapters were then ligated to the RNAs as well. The ligation products were reverse  transcribed by PCR amplification and the 140-160bp size PCR products were enriched to  generate a cDNA library and sequenced using Illumina HiSeq Xten by Gene Denovo  Biotechnology Co. (Guangzhou, China).
characteristics.characteristic: description
characteristics.text: Day of delivery
characteristics.characteristic: developmental stage
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: T6271
characteristics.characteristic: individual
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Bos taurus
characteristics.characteristic: organism
characteristics.text: Bos taurus
characteristics.characteristic: organism
characteristics.text: placenta
characteristics.characteristic: organism part
characteristics.text: Bos taurus
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.characteristic: sex
characteristics.text: BLcattle
characteristics.characteristic: strain
characteristics.text: T6271
characteristics.characteristic: title