characteristics.text: 2025-11-25
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: Institute of Plant Protection
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS27171840
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-15943:OE_2
characteristics.characteristic: Submitter Id
characteristics.text: 17
characteristics.unit: day
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: bread wheat
characteristics.characteristic: common name
characteristics.text: Jimai22
characteristics.characteristic: cultivar
characteristics.text: Protocols: Cereal cyst nematode H. avenae (Treub) Chitwood were collected from an infested wheat field in Qingdao, China. The roots of Wild type and overexpressed wheat were inoculated with Ha nematodes, and the roots were collected 3 days later, with no inoculation as a negative control. The wheat cultivar Fielder was used for transgenic studies, plant-nematode interaction analyses, and parasitism assays.Both plant species were maintained in growth chambers under controlled conditions (23°C, 16-h light/8-h dark photoperiod). First, collect cyst nematodes (Heterodera avenae) from an infested field in Qingdao, China. Then, inoculate wild-type and overexpression wheat roots with these nematodes, while maintaining a non-inoculated set of plants as a negative control. Finally, collect all root samples three days after inoculation. Total RNA was extracted using TRIzol reagent (Invitrogen, catalogue number 15596018). First-strand cDNA was synthesized from 1 μg of total RNA using the PrimeScriptTM RT reagent Kit with gDNA Eraser (Takara).  First, fragmented DNA undergoes end repair and A-tailing to create compatible ends. Then, sequencing adapters are ligated to the fragments, followed by purification and PCR amplification to generate the final library.
characteristics.characteristic: description
characteristics.text: seedling development stage
characteristics.characteristic: developmental stage
characteristics.text: OE
characteristics.characteristic: genotype
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: none
characteristics.characteristic: infect
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Triticum aestivum
characteristics.characteristic: organism
characteristics.text: Triticum aestivum
characteristics.characteristic: organism
characteristics.text: root
characteristics.characteristic: organism part
characteristics.text: Triticum aestivum
characteristics.characteristic: scientific_name
characteristics.text: OE_2
characteristics.characteristic: title