characteristics.text: ERS13598280
characteristics.characteristic: SRA accession
characteristics.text: LBN002:LRC_SSD_IG_24664
characteristics.characteristic: biological material ID
characteristics.text: IPK_LIMS:2638772
characteristics.characteristic: biological material ID
characteristics.text: AGENT:AB_04181
characteristics.ontologyTerms: https://agent.ipk-gatersleben.de/apex/agent/r/agent/accession-details-agent-id?agent_id=AB_04181
characteristics.characteristic: biological material ID
characteristics.text: not collected
characteristics.characteristic: biological material altitude
characteristics.text: not collected
characteristics.characteristic: biological material coordinates uncertainty
characteristics.text: Lebanon
characteristics.characteristic: biological material geographic location
characteristics.text: not collected
characteristics.characteristic: biological material latitude
characteristics.text: not collected
characteristics.characteristic: biological material longitude
characteristics.text: diploid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0001394
characteristics.characteristic: biological material ploidy
characteristics.text: This is a single seed descent (SSD) derived during 2021 from the accession number IG 24664 maintained by International Centre for Agricultural Research in Dry Areas, Beirut, Lebanon
characteristics.characteristic: biological material preprocessing
characteristics.text: not collected
characteristics.characteristic: biological material sowing date
characteristics.text: BSDC00002
characteristics.characteristic: checklist
characteristics.text: not collected
characteristics.characteristic: collected by
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: Hordeum
characteristics.characteristic: genus
characteristics.text: Hordeum vulgare
characteristics.characteristic: infraspecific name
characteristics.text: LBN002:IG 24664
characteristics.characteristic: material source ID
characteristics.text: not collected
characteristics.characteristic: material source altitude
characteristics.text: not collected
characteristics.characteristic: material source coordinates uncertainty
characteristics.text: not collected
characteristics.characteristic: material source description
characteristics.text: not collected
characteristics.characteristic: material source geographic location
characteristics.text: not collected
characteristics.characteristic: material source latitude
characteristics.text: not collected
characteristics.characteristic: material source longitude
characteristics.text: Hordeum vulgare
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4513
characteristics.characteristic: organism
characteristics.text: leaf
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0025034
characteristics.characteristic: plant anatomical entity
characteristics.text: AGENT - Activated Genebank Network
characteristics.characteristic: project name
characteristics.text: 2021-09-03
characteristics.characteristic: record time IPK LIMS
characteristics.text: Individual plants/genotypes were grown in a greenhouse for 2 weeks using propagation trays. Leaf segments were placed in 2 ml tubes (in cool ice) and stored at -80°C. The leaf samples were then lyophilized at -45°C to -50°C for 3 days. 20mg of lyophilized sample was homogenized using the GenoGrinder. DNA was extracted using the CTAB protocol. After the precipitation with isopropanol, the collected DNA was washed 2 times with ethanol and then dried at room temperature overnight inside a fume hood.
characteristics.characteristic: sample description
characteristics.text: vulgare
characteristics.characteristic: species