characteristics.text: ERS16022022
characteristics.characteristic: SRA accession
characteristics.text: 2 weeks
characteristics.characteristic: age
characteristics.text: 22/15 degree Celsius
characteristics.characteristic: air temperature regimen
characteristics.text: not collected
characteristics.characteristic: biological material altitude
characteristics.text: Israel
characteristics.characteristic: biological material geographic location
characteristics.text: 32.1125
characteristics.characteristic: biological material latitude
characteristics.text: 34.805
characteristics.characteristic: biological material longitude
characteristics.text: diploid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0001394
characteristics.characteristic: biological material ploidy
characteristics.text: Seeds of resistant wheat-Ae. sharonensis introgression line D42 were treated with EMS (ethyl methanesulfonate) and screened for screened for susceptible introgression mutant lines.
characteristics.characteristic: biological material preprocessing
characteristics.text: BSDC00001
characteristics.characteristic: checklist
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: The M1 seeds were grown in the greenhouse with controlled conditions of 14 h light and 23/18 ��C day/night temperature, and the seeds of M2 families (single heads) were collected. Ten seeds per family were phenotyped sequentially with P. triticina isolate #526-24 and P. striiformis isolate #5006. The M3 seeds derived from susceptible M2 plants were also tested to confirm that the M2 susceptible plants were true mutants. DNA of all the susceptible M3 mutants was validated for presence of the introgression segment containing the resistance gene by PCR markers. Susceptible mutants derived from independent M4 families were selected for MutChromSeq.
characteristics.characteristic: description
characteristics.text: wheat-Ae. sharonensis introgression mutant line R20_2
characteristics.characteristic: genotype
characteristics.text: Triticum
characteristics.characteristic: genus
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: greenhouse
characteristics.characteristic: growth facility
characteristics.text: not collected
characteristics.characteristic: material source geographic location
characteristics.text: Triticum aestivum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4565
characteristics.characteristic: organism
characteristics.text: leaf
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0025034
characteristics.characteristic: plant anatomical entity
characteristics.text: seedling
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0007131
characteristics.characteristic: plant structure development stage
characteristics.text: Lr/Yr548 RNA
characteristics.characteristic: project
characteristics.text: IPK_LIMS:3196127
characteristics.characteristic: sample id
characteristics.text: aestivum
characteristics.characteristic: species
characteristics.text: 24h after infection
characteristics.characteristic: time