characteristics.text: ERS14314222
characteristics.characteristic: SRA accession
characteristics.text: Koshiki
characteristics.characteristic: accession name
characteristics.text: 26/21 degree Celsius
characteristics.characteristic: air temperature regimen
characteristics.text: 112 m
characteristics.characteristic: biological material altitude
characteristics.text: Germany
characteristics.characteristic: biological material geographic location
characteristics.text: Chionographis japonica PacBio
characteristics.characteristic: biological material id
characteristics.text: 51.816000
characteristics.characteristic: biological material latitude
characteristics.text: 11.283000
characteristics.characteristic: biological material longitude
characteristics.text: diploid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0001394
characteristics.characteristic: biological material ploidy
characteristics.text: BSDC00001
characteristics.characteristic: checklist
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: Chionographis
characteristics.characteristic: genus
characteristics.text: Japan
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: root culture
characteristics.characteristic: growth facility
characteristics.text: PacBio
characteristics.characteristic: library type
characteristics.text: 16h light/8h dark
characteristics.characteristic: light regimen
characteristics.text: Japan
characteristics.characteristic: material source geographic location
characteristics.text: Chionographis japonica
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_119999
characteristics.characteristic: organism
characteristics.text: root
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0009005
characteristics.characteristic: plant anatomical entity
characteristics.text: Chionographis japonica Sequencing
characteristics.characteristic: project name
characteristics.text: In vitro root cultures of C. japonica were induced from leaf petioles. After gentle washing with water, petioles were surface sterilized with a diluted sodium hypochlorite solution (3% active chlorine) supplemented with two drops of Tween 20 for 15 min, followed by a four-times rinse in autoclaved distilled 1 water. Afterwards, petioles were cut into 3 mm segments under sterile conditions and cultivated on �� Macro Murashige/Skoog (�� MS) medium (MURASHIGE AND SKOOG 1962) supplemented with 10.74 ��M NAA, 0.44 ��M BAP, 3% sucrose, and 0.8% Micro Agar, pH 5.8, in Petri dishes. The parameter in the growth chamber was 16 h light exposure at 26 ��C followed by 8 h darkness at 21 ��C. To avoid light stress, the explants were shadowed with paper sheets for two weeks. Three weeks later, the petiole segments were transferred to fresh medium. After additional six weeks, roots formed on the petiole segments were separated, multiplied, and subcultured on �� MS medium supplemented with 2.69 ��M NAA, 20% sucrose, 1g/l peptone, 230 mg/l NaH2PO4 x 2 H2O and 2.5 g/l PhytagelTM, pH 5.2, under the same growing conditions. Root tuffs were subcultured on fresh medium every 4 - 6 weeks.
characteristics.characteristic: sample description
characteristics.text: japonica
characteristics.characteristic: species