characteristics.text: 2023-11-02
characteristics.characteristic: ENA first public
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS16474575
characteristics.characteristic: SRA accession
characteristics.text: 20 degree C day/15 degree C night
characteristics.characteristic: air temperature regimen
characteristics.text: 112m
characteristics.characteristic: biological material altitude
characteristics.text: 2000m
characteristics.characteristic: biological material coordinates uncertainty
characteristics.text: Germany
characteristics.characteristic: biological material geographic location
characteristics.text: 51.816
characteristics.characteristic: biological material latitude
characteristics.text: 11.283
characteristics.characteristic: biological material longitude
characteristics.text: diploid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0001394
characteristics.characteristic: biological material ploidy
characteristics.text: BSDC00001
characteristics.characteristic: checklist
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: BW-NIL-flo.a
characteristics.characteristic: genotype
characteristics.text: Hordeum
characteristics.characteristic: genus
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: 16h light/8h dark
characteristics.characteristic: light regimen
characteristics.text: not collected
characteristics.characteristic: material source geographic location
characteristics.text: Hordeum vulgare
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4513
characteristics.characteristic: organism
characteristics.text: Spike
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0009051
characteristics.characteristic: plant anatomical entity
characteristics.text: WA
characteristics.characteristic: plant structure development stage
characteristics.text: Barley paired spikelet transcriptome
characteristics.characteristic: project
characteristics.text: rep3
characteristics.characteristic: replicate
characteristics.text: Bowman and BW-NIL-flo.a were grown in a phytochamber condition (light/dark: 16h/8h; day/night temperature: 20 degree /15 degree ) in a 24-well plate. Spike samples at Waddington stages W2.0 (Double Ridge), W3.0 (Lemma Primordium ), W4.5 (Awn primordium) and W5.5 (White Anther) stages were collected under a light microscope. , the whole spike was crossed-sectioned into two parts (upper-mid and basal) at stages W3.0, W4.5 and W5.5 under a stereoscopic microscope.A total of 15-48 spikes per sample and three biological replicates were harvested and used for RNA extraction. All sample collections were conducted between 11:00am ��� 14:00pm within a day.
characteristics.characteristic: sample description
characteristics.text: IPK_LIMS:3219115,PSB_ID:R27
characteristics.characteristic: sample id
characteristics.text: vulgare
characteristics.characteristic: species
characteristics.text: Basal
characteristics.characteristic: tissue position
characteristics.text: W5.5
characteristics.characteristic: waddington