characteristics.text: SAMD01687755
characteristics.characteristic: External Id
characteristics.text: Department of Ophthalmology, National Defense Medical College
characteristics.characteristic: INSDC center name
characteristics.text: 2025-09-25T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2025-09-24T18:43:04Z
characteristics.characteristic: INSDC last update
characteristics.text: DRS524547
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: DRS524547
characteristics.characteristic: SRA accession
characteristics.text: 11_weeks
characteristics.characteristic: age
characteristics.text: Japan SLC
characteristics.characteristic: biomaterial_provider
characteristics.text: missing
characteristics.characteristic: breed
characteristics.text: retinal cells
characteristics.characteristic: cell_type
characteristics.text: 2021-12-21
characteristics.characteristic: collection_date
characteristics.text: missing
characteristics.characteristic: cultivar
characteristics.text: ThThis sample consists of retinal single-cells isolated from the retinas of C57BL/6J (B6) mice with experimental autoimmune uveoretinitis (EAU) induced by human IRBP 1-20 (WT-EAU). The method for preparing the sample is described in the following reference: https://doi.org/10.3390/biomedicines13092049. Briefly, eight-week-old female B6 mice were subcutaneously immunized in the lumbar region with an emulsion of human IRBP 1-20 in PBS and CFA. To enhance the immune response, pertussis toxin was administered intraperitoneally. Three weeks after immunization, the mice were euthanized, and their retinas were enucleated. The retinas were then enzymatically digested with papain to obtain single-cell suspensions. Rod cells were subsequently removed using magnetic beads targeting CD74, and single-cell RNA sequencing was performed on the remaining retinal cells.
characteristics.characteristic: description
characteristics.text: missing
characteristics.characteristic: dev_stage
characteristics.text: experimental autoimmune uveoretinitis
characteristics.characteristic: disease
characteristics.text: missing
characteristics.characteristic: ecotype
characteristics.text: Japan
characteristics.characteristic: geo_loc_name
characteristics.text: missing
characteristics.characteristic: isolate
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: WT-EAU
characteristics.characteristic: sample_name
characteristics.text: female
characteristics.characteristic: sex
characteristics.text: C57BL6J
characteristics.characteristic: strain
characteristics.text: retina
characteristics.characteristic: tissue
characteristics.text: WT-EAU
characteristics.characteristic: title