characteristics.text: ERS16294070
characteristics.characteristic: SRA accession
characteristics.text: not collected
characteristics.characteristic: accession name
characteristics.text: not collected
characteristics.characteristic: age
characteristics.text: 112m
characteristics.characteristic: biological material altitude
characteristics.text: 2000m
characteristics.characteristic: biological material coordinates uncertainty
characteristics.text: Germany
characteristics.characteristic: biological material geographic location
characteristics.text: FB19_011_3
characteristics.characteristic: biological material id
characteristics.text: 51.816000
characteristics.characteristic: biological material latitude
characteristics.text: 11.283000
characteristics.characteristic: biological material longitude
characteristics.text: diploid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0001394
characteristics.characteristic: biological material ploidy
characteristics.text: not applicable
characteristics.characteristic: biological material preprocessing
characteristics.text: BSDC00001
characteristics.characteristic: checklist
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: Hordeum
characteristics.characteristic: genus
characteristics.text: Greece
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: greenhouse
characteristics.characteristic: growth facility
characteristics.text: RNAseq
characteristics.characteristic: library type
characteristics.text: not collected
characteristics.characteristic: material source altitude
characteristics.text: not collected
characteristics.characteristic: material source description
characteristics.text: Greece
characteristics.characteristic: material source geographic location
characteristics.text: not collected
characteristics.characteristic: material source latitude
characteristics.text: not collected
characteristics.characteristic: material source longitude
characteristics.text: Hordeum bulbosum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4516
characteristics.characteristic: organism
characteristics.text: root
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0009005
characteristics.characteristic: plant anatomical entity
characteristics.text: not collected
characteristics.characteristic: plant structure development stage
characteristics.text: Hbulbosum pangenome
characteristics.characteristic: project name
characteristics.text: Clones of individual FB19-011-3 were grown in a greenhouse at the Leibniz Institute of Plant Genetics and Crop Plant Research. Tissue samples of root, bulb, stem, leaf, stalk, flag leaf and young inflorescences (including flowers) were harvested frozen in liquid nitrogen and stored at -80 degree C. Both leaf tissues were obtained at the end of the night to reduce the number of photosynthesis-related transcripts in the extracted RNA. Total RNA was isolated with the RNeasy Plant Mini kit (Qiagen, Germany) following the instructions provided by the manufacturer. RNAs of the single-tissue samples were pooled for Iso-Seq.
characteristics.characteristic: sample description
characteristics.text: IPK_LIMS:2497435
characteristics.characteristic: sample id
characteristics.text: 2021-04-28
characteristics.characteristic: sequencing date ipk
characteristics.text: bulbosum
characteristics.characteristic: species