characteristics.text: ERS8446198
characteristics.characteristic: SRA accession
characteristics.text: 22/18 degree Celsius
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/UO_0000027
characteristics.characteristic: air temperature regimen
characteristics.text: 112 m
characteristics.characteristic: biological material altitude
characteristics.text: Germany
characteristics.characteristic: biological material geographic location
characteristics.text: 51.816
characteristics.characteristic: biological material latitude
characteristics.text: 11.283
characteristics.characteristic: biological material longitude
characteristics.text: allopolyploidy
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0001379
characteristics.characteristic: biological material ploidy
characteristics.text: BSDC00001
characteristics.characteristic: checklist
characteristics.text: 2017-07-31
characteristics.characteristic: collection date
characteristics.text: Darmor-PBY018
characteristics.characteristic: cultivar
characteristics.text: Brassica napus cv. Darmor-PBY018 plants were cultivated in B-deficient soil substrate in the glasshouse. A B-free white-peat volcanic clay mixture (as from now called zerosoil-substrate) was supplemented with 0.5% CaCO3 and 0.3% CaO by mixing 20 kg batches of soil with 1 L of CaCO3 (100 g L-1) and 1 L of CaO (60 g L-1) in a clean cement mixer (Lescha Typ SBM P 150L) for 10 min. Thereafter, soil substrate was manually mixed every other day. Around 1-2 weeks after preparation, clumps were sieved out and the soil (dry matter: ~ 30 %) was used for the experiments. Rapeseed plants were germinated directly in individual pots (9 cm) filled with zerosoil-substrate in the glasshouse. Pots were watered every two days with 250 ml 1x modified Hoagland solution containing 200 ��M boric acid. All solutions additionally contained the following concentrations of nutrients: 7.5 mM NH4NO3, 2.9 mM KH2PO4, 850 ��M MgSO4, 340 ��M K2SO4, 320 ��M MnCl2, 52 ��M CuSO4, 20 ��M NaFeEDTA, 4.5 ��M ZnSO4, 0.4 ��M NaMoO4. At BBCH15, plants were transplanted into new pots (16 cm) with fresh zerosoil-substrate. Plants under B-deficient conditions were treated in the same way but without the addition of any B. Nutrients were supplied to the soil weekly. Care was taken to avoid transfer of soil by roots during transplantation. Plants were then subjected to vernalization for six weeks at 6��C, and finally transferred to the glasshouse. No glassware was used in any process during the preparation of the nutrient solution or the irrigation of the plants to prevent additional input of B into the growth system. Glasshouse conditions were set to long-day conditions. Plant tissues from three individual plants per B growth condition were harvested for RNA isolation. At BBCH 51, the shoot head (including the shoot apical meristem and the forming flower buds) as well as 2-3 bracts were sampled.
characteristics.characteristic: description
characteristics.text: Brassica
characteristics.characteristic: genus
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: greenhouse
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/ENVO_00005780
characteristics.characteristic: growth facility
characteristics.text: 50/60% relative humidity
characteristics.characteristic: humidity regimen
characteristics.text: 250 ��mol/m2 s
characteristics.characteristic: light intensity
characteristics.text: 16h light/8h dark
characteristics.characteristic: light regimen
characteristics.text: not collected
characteristics.characteristic: material source geographic location
characteristics.text: Brassica napus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3708
characteristics.characteristic: organism
characteristics.text: shoot head
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0000229
characteristics.characteristic: plant anatomical entity
characteristics.text: B-free white-peat volcanic clay mixture
characteristics.characteristic: plant growth medium
characteristics.text: BBCH60-61
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0007026
characteristics.characteristic: plant structure development stage
characteristics.text: Boron deficiency responsive genes in Brassica napus inflorescences
characteristics.characteristic: project
characteristics.text: 2
characteristics.characteristic: replicate
characteristics.text: Pots were watered every two days with 250 ml 1x modified Hoagland solution containing 200 ��M boric acid. All solutions additionally contained the following concentrations of nutrients: 7.5 mM NH4NO3, 2.9 mM KH2PO4, 850 ��M MgSO4, 340 ��M K2SO4, 320 ��M MnCl2, 52 ��M CuSO4, 20 ��M NaFeEDTA, 4.5 ��M ZnSO4, 0.4 ��M NaMoO4. At BBCH15, plants were transplanted into new pots with fresh soil. Plants under B-sufficient conditions were maintained under the same watering and fertilization regime as above. Plants under B-deficient conditions were treated in the same way but without the addition of any B. Nutrients were supplied to the soil weekly.
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PECO_0007405
characteristics.characteristic: rooting medium micronutrients
characteristics.text: IPK_LIMS:1743403
characteristics.characteristic: sample id
characteristics.text: napus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3708
characteristics.characteristic: species
characteristics.text: minus Bor
characteristics.characteristic: treatment