characteristics.text: ERS24837251
characteristics.characteristic: SRA accession
characteristics.text: AGENT:AW_01237
characteristics.ontologyTerms: https://agent.ipk-gatersleben.de/apex/agent/r/agent/accession-details-agent-id?agent_id=AW_01237
characteristics.characteristic: biological material ID
characteristics.text: IPK_LIMS:2753485
characteristics.characteristic: biological material ID
characteristics.text: CHE001:2020PA-563_UZH#1_GB-7110
characteristics.characteristic: biological material ID
characteristics.text: not collected
characteristics.characteristic: biological material altitude
characteristics.text: not collected
characteristics.characteristic: biological material coordinates uncertainty
characteristics.text: Switzerland
characteristics.characteristic: biological material geographic location
characteristics.text: not collected
characteristics.characteristic: biological material latitude
characteristics.text: not collected
characteristics.characteristic: biological material longitude
characteristics.text: hexaploid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0001384
characteristics.characteristic: biological material ploidy
characteristics.text: This is a single seed descent (SSD) derived during 2021 from the accession number 7110 maintained by Agroscope Changins, Nyon, Switzerland
characteristics.characteristic: biological material preprocessing
characteristics.text: BSDC00002
characteristics.characteristic: checklist
characteristics.text: not collected
characteristics.characteristic: collected by
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: Triticum
characteristics.characteristic: genus
characteristics.text: Triticum aestivum
characteristics.characteristic: infraspecific name
characteristics.text: CHE001:7110
characteristics.characteristic: material source ID
characteristics.text: not collected
characteristics.characteristic: material source altitude
characteristics.text: not collected
characteristics.characteristic: material source coordinates uncertainty
characteristics.text: not collected
characteristics.characteristic: material source description
characteristics.text: Switzerland
characteristics.characteristic: material source geographic location
characteristics.text: not collected
characteristics.characteristic: material source latitude
characteristics.text: not collected
characteristics.characteristic: material source longitude
characteristics.text: Triticum aestivum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4565
characteristics.characteristic: organism
characteristics.text: leaf
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0025034
characteristics.characteristic: plant anatomical entity
characteristics.text: AGENT - Activated Genebank Network
characteristics.characteristic: project name
characteristics.text: 2021-11-12
characteristics.characteristic: record time IPK LIMS
characteristics.text: Seeds were provided by CHE001. Individual plants for DNA extraction were grown in a climate chamber at UZH. Two segments of the first leaf (each approximately 3 cm in length and 0.3 to 0.5 cm wide) were placed in 2.2 ml tubes together with two 4mm glass beads (ROTH). Samples were frozen in liquid nitrogen and grinded with a Geno/Grinder (SPEX SamplePrep) at 1500 rpm for 1 minute. DNA was extracted with Serapure magnetic beads according to Baptiste Mayjonade et al., Extraction of high-molecular-weight genomic DNA for the long-read sequencing Biotechniques, 2016. Purified DNA was disolved in 10mM Tris HCl pH 8.0 and shipped frozen to IHAR with blue ice.
characteristics.characteristic: sample description
characteristics.text: aestivum
characteristics.characteristic: species