characteristics.text: ERS21171257
characteristics.characteristic: SRA accession
characteristics.text: Wonjam1-ho
characteristics.characteristic: accession name
characteristics.text: USDA:PI469181
characteristics.characteristic: accession number
characteristics.text: not collected
characteristics.characteristic: age
characteristics.text: 112 m
characteristics.characteristic: biological  material altitude
characteristics.text: 2000 m
characteristics.characteristic: biological  material coordinates uncertainty
characteristics.text: 230-5-cont_r3
characteristics.characteristic: biological material ID
characteristics.text: Germany
characteristics.characteristic: biological material geographic location
characteristics.text: 51.816
characteristics.characteristic: biological material latitude
characteristics.text: 11.283
characteristics.characteristic: biological material longitude
characteristics.text: diploid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0001394
characteristics.characteristic: biological material ploidy
characteristics.text: BSDC00002
characteristics.characteristic: checklist
characteristics.text: 2024-06-11
characteristics.characteristic: collection date
characteristics.text: Vicia
characteristics.characteristic: genus
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: greenhouse
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/ENVO_03600087
characteristics.characteristic: growth facility
characteristics.text: RNA-Seq
characteristics.characteristic: library type
characteristics.text: not collected
characteristics.characteristic: material source altitude
characteristics.text: not collected
characteristics.characteristic: material source description
characteristics.text: South Korea
characteristics.characteristic: material source geographic location
characteristics.text: not collected
characteristics.characteristic: material source latitude
characteristics.text: not collected
characteristics.characteristic: material source longitude
characteristics.text: Vicia Faba
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_3906
characteristics.characteristic: organism
characteristics.text: leaf
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0025034
characteristics.characteristic: plant anatomical entity
characteristics.text: not collected
characteristics.characteristic: plant structure development stage
characteristics.text: faba_bean_RNA-Seq
characteristics.characteristic: project name
characteristics.text: 3
characteristics.characteristic: replicate
characteristics.text: IPK_LIMS:3360690
characteristics.characteristic: sample ID
characteristics.text: Wonjam1-ho was developed from PI 469181, which is a new variety with superior adaptability to the winter environmental conditions in South Korea. This variety was generated by irradiation with 100 Gy gamma-rays using a Co gamma irradiator (150 TBq capacity; ACEL, Ottawa, ON, Canada).  To investigate the transcriptome response for frost tolerance between Wonjam1-ho (tolerant), plants were placed in 15 cm plastic pots with commercial soil (Hungnong, Pyeongtaek, Republic of Korea) and were grown in a growth chamber (Vision Science, Republic of Korea) set at 18 ℃ for 20 days. Next, the temperature in the growth chamber was gradually decreased at a rate of 1 °C/h until it reached 4 °C, where it was held for 3 days (12-h day/12-h night). The temperature was further lowered by 1 °C/h to −7 °C, maintained for 12-h night, and then raised in reverse order back to 18 °C for recovery. Leaves were collected after treatments (at 0 °C), immediately frozen in liquid nitrogen, and used for RNA extraction.And Each sample has 3 replicates.
characteristics.characteristic: sample description
characteristics.text: Faba
characteristics.characteristic: species