characteristics.text: ERS17839227
characteristics.characteristic: SRA accession
characteristics.text: bridging
characteristics.characteristic: accession name
characteristics.text: CHE001:CHE001_GB-2466
characteristics.characteristic: biological material ID
characteristics.text: AGENT:AW_13234
characteristics.ontologyTerms: https://agent.ipk-gatersleben.de/apex/agent/r/agent/accession-details-agent-id?agent_id=AW_13234
characteristics.characteristic: biological material ID
characteristics.text: not collected
characteristics.characteristic: biological material altitude
characteristics.text: not collected
characteristics.characteristic: biological material coordinates uncertainty
characteristics.text: Switzerland
characteristics.characteristic: biological material geographic location
characteristics.text: not collected
characteristics.characteristic: biological material latitude
characteristics.text: not collected
characteristics.characteristic: biological material longitude
characteristics.text: hexaploid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0001384
characteristics.characteristic: biological material ploidy
characteristics.text: This is a single seed descent (SSD) derived during 2023 from the accession number 2466 maintained by Agroscope Changins, Nyon, Switzerland
characteristics.characteristic: biological material preprocessing
characteristics.text: BSDC00002
characteristics.characteristic: checklist
characteristics.text: not collected
characteristics.characteristic: collected by
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: Triticum
characteristics.characteristic: genus
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: Triticum aestivum
characteristics.characteristic: infraspecific name
characteristics.text: CHE001:2466
characteristics.characteristic: material source ID
characteristics.text: not collected
characteristics.characteristic: material source altitude
characteristics.text: not collected
characteristics.characteristic: material source coordinates uncertainty
characteristics.text: not collected
characteristics.characteristic: material source description
characteristics.text: not collected
characteristics.characteristic: material source geographic location
characteristics.text: not collected
characteristics.characteristic: material source latitude
characteristics.text: not collected
characteristics.characteristic: material source longitude
characteristics.text: Triticum aestivum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4565
characteristics.characteristic: organism
characteristics.text: leaf
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0025034
characteristics.characteristic: plant anatomical entity
characteristics.text: AGENT - Activated Genebank Network
characteristics.characteristic: project name
characteristics.text: 2023-09-29
characteristics.characteristic: record time IPK LIMS
characteristics.text: IPK_LIMS:3222122
characteristics.characteristic: sample ID
characteristics.text: Leaf material provided by CHE001. Individual plants for DNA extraction were grown in greenhouse at CHE001. Approx. 75 mg of first leaves were placed in cluster tubes (96 well) together with one glass bead (ROTH). Samples were frozen in liquid nitrogen and  grinded with a Geno/Grinder (SPEX SamplePrep) at 1500 rpm for 1 minute. DNA was extracted with Serapure magnetic beads KingFisher Apex adapted from Baptiste Mayjonade et al., Extraction of high-molecular-weight genomic DNA for the long-read sequencing Biotechniques, 2016. Purified DNA was disolved in 10mM Tris HCl pH 8.0 and shipped frozen to IHAR  with blue ice. IHAR received DNA labeled "Agent UZH-III, plate 1-4" on 8.7.2022.
characteristics.characteristic: sample description
characteristics.text: aestivum
characteristics.characteristic: species