characteristics.text: 2025-11-26
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: The Ragon Institute of Mass General Brigham, MIT, and Harvard
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS27250996
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-16123:VDJ_S1
characteristics.characteristic: Submitter Id
characteristics.text: 15
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: germinal center B cell; naive B cell; Plasma B cell; memory B cell;
characteristics.characteristic: cell type
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: FIP200f/fMb1Cre+/- (KO) mice and their littermatecontrol, FIP200f/fMb1Cre-/-(WT) were immunized with 50 ug NP29-KLH with Alum, then splenocytes isolated from WT and KO mice 11 days after immunization were enriched for GC, plasma, and memory B cells by depleting non-B cells and IgDhi B cells using magnetic beads. Those enriched samples were then barcoded and sorted for naïve, GC, plasma, and memory B cells, which were then mixed for single-cell RNA sequencing. Mixed B cells were resuspended in the master mix and loaded together with partitioning oil and gel beads into the chip to generate the gel bead-in-emulsion (GEM). Library was performed according to the manufactor's instruction (Chromium Single-Cell 5′ Reagent Kit v2 Chemistry, Dual Index). Briefly, the poly-A RNA form the cell lysate contained in every signle GEM was retrotranscripted to cDNA, which contains an Illumina R1 primer sequence, the 10x Barcode and Unique Molecular Identifier (UMI). The pooled barcodeed cDNA was then cleaned up with Sliane DynaBeads, amplified by PCR and the apropiated sized fragmnets were selected with SPRIselect reagent for subsequent library constrution. During the library construction, Illumina R2 primer sequence, paired-end constructs with P5 and P7 sequences and Sample index i5 and i7 were added.
characteristics.characteristic: description
characteristics.text: Mature
characteristics.characteristic: developmental stage
characteristics.text: Fip200flox/flox Mb1Cre-/-
characteristics.characteristic: genotype
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: WT
characteristics.characteristic: individual
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: spleen
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.characteristic: sex
characteristics.text: Fip200flox/floxMb1Cre-/-
characteristics.characteristic: strain
characteristics.text: VDJ_S1
characteristics.characteristic: title