characteristics.text: 2025-11-26
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: Xiamen university
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS27784061
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-16271:WT2
characteristics.characteristic: Submitter Id
characteristics.text: 12
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Mice were euthanized by cervical dislocation at 12 weeks of age. The liver tissue was immediately dissected, rapidly frozen in liquid nitrogen, and subsequently stored at -80°C. Animal samples were extracted using the Trizol method. The extracted RNA was dissolved in 50 μL of DEPC-treated water. The quantity and quality of the total RNA were assessed using a Qubit fluorometer and a Qsep400 high-throughput biofragment analyzer. Strand-specific RNA-seq libraries were prepared. Briefly, mRNA was enriched from total RNA using Oligo(dT) magnetic beads. The purified mRNA was fragmented using fragmentation buffer at an elevated temperature. First-strand cDNA was synthesized using random hexamer primers. Second-strand cDNA was synthesized incorporating dUTP in place of dTTP to achieve strand specificity, followed by end repair, A-tailing, and adapter ligation. Libraries were size-selected for insert sizes of 250-350 bp using DNA magnetic beads and then amplified by PCR. Final library quality was assessed using a Qubit fluorometer and a Qsep400 analyzer. For the DNB platform, the double-stranded DNA library was circularized to form single-stranded circular DNA, which was then rolled-circle amplified to produce DNA nanoballs (DNBs).
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: fasting
characteristics.characteristic: diet
characteristics.text: no
characteristics.characteristic: disease
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: WT2
characteristics.characteristic: individual
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: liver
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: C57BL/6J
characteristics.characteristic: strain
characteristics.text: WT2
characteristics.characteristic: title