characteristics.text: 2025-11-26
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: BIODK
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS27363949
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-16209:Sample3
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: Primary cells
characteristics.characteristic: cell line
characteristics.text: Human bronchial epithelial cells
characteristics.characteristic: cell type
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Cells were grown according to the protocol in DOI 10.1385/1-59259-861-7:183 Passage 2 primary human bronchial epithelial (HBE) cells  were cultured in air-liquid interface (ALI) culture for up to 14 days in culture. HBE cells were grown normally according to the growth protocol above and frozen on ALI days 0, 1, 7, and 14 to allow for RNA extraction. Total RNA was isolated from HBE cells frozen on ALI days 0, 1, 7, and 14 using the TRIzol Plus RNA Purification Kit (Invitrogen) Sequencing libraries were constructed with a Watchmaker Genomics kit.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: no lung disease
characteristics.characteristic: disease
characteristics.text: NA
characteristics.characteristic: genotype
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: VM
characteristics.characteristic: growth condition
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: lung epithelium
characteristics.characteristic: organism part
characteristics.text: 3
characteristics.characteristic: replicate
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: Sample3
characteristics.characteristic: title