characteristics.text: ERS17838459
characteristics.characteristic: SRA accession
characteristics.characteristic: accession name
characteristics.text: AGENT:AW_08872
characteristics.ontologyTerms: https://agent.ipk-gatersleben.de/apex/agent/r/agent/accession-details-agent-id?agent_id=AW_08872
characteristics.characteristic: biological material ID
characteristics.text: ITA383:AGENT_SSD_TA00795
characteristics.characteristic: biological material ID
characteristics.text: not collected
characteristics.characteristic: biological material altitude
characteristics.text: not collected
characteristics.characteristic: biological material coordinates uncertainty
characteristics.text: Italy
characteristics.characteristic: biological material geographic location
characteristics.text: not collected
characteristics.characteristic: biological material latitude
characteristics.text: not collected
characteristics.characteristic: biological material longitude
characteristics.text: hexaploid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0001384
characteristics.characteristic: biological material ploidy
characteristics.text: This is a single seed descent (SSD) derived during 2023 from the accession number TA00795 maintained by CRA-Unitá di Ricerca per la Risicoltura, Vercelli, Italy
characteristics.characteristic: biological material preprocessing
characteristics.text: BSDC00002
characteristics.characteristic: checklist
characteristics.text: not collected
characteristics.characteristic: collected by
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: Triticum
characteristics.characteristic: genus
characteristics.text: Italy
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: Triticum aestivum
characteristics.characteristic: infraspecific name
characteristics.text: ITA383:TA00795
characteristics.characteristic: material source ID
characteristics.text: not collected
characteristics.characteristic: material source altitude
characteristics.text: not collected
characteristics.characteristic: material source coordinates uncertainty
characteristics.text: not collected
characteristics.characteristic: material source description
characteristics.text: Italy
characteristics.characteristic: material source geographic location
characteristics.text: not collected
characteristics.characteristic: material source latitude
characteristics.text: not collected
characteristics.characteristic: material source longitude
characteristics.text: Triticum aestivum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4565
characteristics.characteristic: organism
characteristics.text: leaf
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0025034
characteristics.characteristic: plant anatomical entity
characteristics.text: AGENT - Activated Genebank Network
characteristics.characteristic: project name
characteristics.text: 2023-09-29
characteristics.characteristic: record time IPK LIMS
characteristics.text: IPK_LIMS:3220737
characteristics.characteristic: sample ID
characteristics.text: Seeds from individual SSD lines were grown in a climatic chamber in 96-well trays for two weeks. Two leaf segments (each approximately 3 cm in length ) were placed in a 96-well polypropylene cluster tube (1.2 ml, 8 tube stripe format; Corning) containing one 2 mm lead bead. The tubes were closed with 8-cap tube strips. Samples were frozen in liquid nitrogen and stored at -80°C until use. Materials were grinded by Tissue Lizer (Quigen) and DNA was extracted with MATAB, cleaned with chloroform-isoamyl-alcohol 24:1, precipitated with isopropanol and washes with 70% Ethanol. After resuspending in Tris 10mM pH 8.4, DNAs were treated with RNAse A pancreatic, quantified by Qubit and checked for integrity by agarose gel electrophoresis.
characteristics.characteristic: sample description
characteristics.text: aestivum
characteristics.characteristic: species