characteristics.text: ERS23302537
characteristics.characteristic: SRA accession
characteristics.text: 2226DP212
characteristics.characteristic: biological material ID
characteristics.text: 112m
characteristics.characteristic: biological material elevation
characteristics.text: Germany
characteristics.characteristic: biological material geographic location
characteristics.text: 51,816
characteristics.characteristic: biological material latitude
characteristics.text: 11,283
characteristics.characteristic: biological material longitude
characteristics.text: diploid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0001394
characteristics.characteristic: biological material ploidy
characteristics.text: BSDC00002
characteristics.characteristic: checklist
characteristics.text: 2022-07-13
characteristics.characteristic: collection date
characteristics.text: Moneymaker
characteristics.characteristic: genotype
characteristics.text: Solanum
characteristics.characteristic: genus
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: phytochamber
characteristics.characteristic: growth facility
characteristics.text: RNAseq
characteristics.characteristic: library type
characteristics.text: 16 h day/8 h night
characteristics.characteristic: light regimen
characteristics.text: not applicable
characteristics.characteristic: material source altitude
characteristics.text: not applicable
characteristics.characteristic: material source description
characteristics.text: not collected
characteristics.characteristic: material source geographic location
characteristics.text: not collected
characteristics.characteristic: material source latitude
characteristics.text: not collected
characteristics.characteristic: material source longitude
characteristics.text: Solanum lycopersicum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4081
characteristics.characteristic: organism
characteristics.text: 20 days
characteristics.characteristic: plant age
characteristics.text: leaf
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0025034
characteristics.characteristic: plant anatomical entity
characteristics.text: leaf production stage
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0007133
characteristics.characteristic: plant structure development stage
characteristics.text: Tomato heat stress response
characteristics.characteristic: project name
characteristics.text: 4
characteristics.characteristic: replicate
characteristics.text: IPK_LIMS:3167152
characteristics.characteristic: sample ID
characteristics.text: Seeds were planted in well-watered soil trays and kept under a plastic cover in a phytochamber with a day/night cycle of 16/8h and temperatures of 24°C and 20°C respectively. The seeds were kept without light for one day, then illumination of 320µmol per square meter per second was added during the day phase. After a total of 7 days after sowing, evenly germinated and healthy seedlings were transplanted into individual pots (10 cm diameter, 8 cm height) without plastic covers at 60-70% relative air humidity. After 9 further days of establishment in the pot, the plants were exposed to heat stress at 37°C/28°C (day/night) for 6 days followed by a recovery phase at 24°C/20°C. Plants were watered once or twice daily by an automated system replenishing evapotranspirated water by weight to ensure the plants do not experience drought stress. The youngest fully formed leaf pair was harvested (directly put in liquid nitrogen and then stored at -80°C) for molecular measurements mid-day (between 13:00 and 14:00, light phase was from 06:00-22:00) from individual plants directly before the temperature switch (day0), each day during the heat stress phase (day1 to day6) and after 1, 2, and 4 days of recovery (day7 to day10). Control plants were grown exactly the same way except that they stayed at 24°C/20°C throughout the whole experiment. They were sampled at the same respective time points as the heat-treated plants.
characteristics.characteristic: sample description
characteristics.text: lycopersicum
characteristics.characteristic: species
characteristics.text: 24 °C/20 °C
characteristics.characteristic: temperatur regimen
characteristics.text: control
characteristics.characteristic: treatment