characteristics.text: 2024-05-13
characteristics.characteristic: ENA first public
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS18895303
characteristics.characteristic: SRA accession
characteristics.text: 112 m
characteristics.characteristic: biological material altitude
characteristics.text: Germany
characteristics.characteristic: biological material geographic location
characteristics.text: CS2B
characteristics.characteristic: biological material id
characteristics.text: 51.816
characteristics.characteristic: biological material latitude
characteristics.text: 11.283
characteristics.characteristic: biological material longitude
characteristics.text: hexaploid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0001384
characteristics.characteristic: biological material ploidy
characteristics.text: not applicable
characteristics.characteristic: biological material preprocessing
characteristics.text: BSDC00001
characteristics.characteristic: checklist
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: Chinese Spring
characteristics.characteristic: cultivar
characteristics.text: Triticum
characteristics.characteristic: genus
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: light  16h/21 degree C and dark 8h/17 degree C
characteristics.characteristic: growth condition
characteristics.text: greenhouse
characteristics.characteristic: growth facility
characteristics.text: 50% relative humidity
characteristics.characteristic: humidity regimen
characteristics.text: not applicable
characteristics.characteristic: ipk sequencing date
characteristics.text: 100 to 120 ��mol/m2 s
characteristics.characteristic: light intensity
characteristics.text: not collected
characteristics.characteristic: material source geographic location
characteristics.text: Triticum aestivum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4565
characteristics.characteristic: organism
characteristics.text: leaf
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0025034
characteristics.characteristic: plant anatomical entity
characteristics.text: not collected
characteristics.characteristic: plant structure development stage
characteristics.text: Genomic DNA of rye and wheat was isolated using the method described as follows; 1.21 g Tris, 4.09 g NaCl, and 1.86 g EDTA were dissolved in dH2O to 100 ml extraction buffer (pH=8.0). A 1 - 3 cm leaf segment was placed into a 2 ml tube containing two beads, frozen with liquid nitrogen, and pulverized by a vibrating mill (MM400, Retsch). 1.2 ml extraction buffer was added to the tube and incubated for 15 minutes at 65 degree C. Then, the tube was cooled down for 1 minute at RT and gently shaken after adding 600 microliter of a chloroform/isopropanol mixture (24:1). The supernatant in the tube was moved to a fresh 1.5 ml tube after centrifugation (13,000 g, 2 min), mixed with 700 microliter of isopropanol, and incubated again for two minutes at RT. The supernatant was discarded after centrifugation, the pellet was washed 2 times with 70 % ethanol by vortexing and centrifugation. To remove the excess liquid, the tube was air-dried until all ethanol was evaporated. Finally, 50 microliter of dH20 is added to dissolve the DNA, and Nanodrop (Thermo Scientific NanoDrop One) was used to determine the DNA concentration.
characteristics.characteristic: sample description
characteristics.text: IPK_LIMS:3279457
characteristics.characteristic: sample id
characteristics.text: aestivum
characteristics.characteristic: species
characteristics.text: 2 standard rye B chromosomes
characteristics.characteristic: treatment