characteristics.text: ERS16364161
characteristics.characteristic: SRA accession
characteristics.text: not collected
characteristics.characteristic: accession name
characteristics.text: not collected
characteristics.characteristic: age
characteristics.text: 112m
characteristics.characteristic: biological material altitude
characteristics.text: 2000m
characteristics.characteristic: biological material coordinates uncertainty
characteristics.text: Germany
characteristics.characteristic: biological material geographic location
characteristics.text: FB19_011_3
characteristics.characteristic: biological material id
characteristics.text: 51.816000
characteristics.characteristic: biological material latitude
characteristics.text: 11.283000
characteristics.characteristic: biological material longitude
characteristics.text: diploid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0001394
characteristics.characteristic: biological material ploidy
characteristics.text: not applicable
characteristics.characteristic: biological material preprocessing
characteristics.text: BSDC00001
characteristics.characteristic: checklist
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: Hordeum
characteristics.characteristic: genus
characteristics.text: Greece
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: not collected
characteristics.characteristic: growth facility
characteristics.text: single cell genomic
characteristics.characteristic: library type
characteristics.text: not collected
characteristics.characteristic: material source altitude
characteristics.text: not collected
characteristics.characteristic: material source description
characteristics.text: Greece
characteristics.characteristic: material source geographic location
characteristics.text: not collected
characteristics.characteristic: material source latitude
characteristics.text: not collected
characteristics.characteristic: material source longitude
characteristics.text: Hordeum bulbosum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4516
characteristics.characteristic: organism
characteristics.text: pollen
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0025281
characteristics.characteristic: plant anatomical entity
characteristics.text: pollen development stage
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0001007
characteristics.characteristic: plant structure development stage
characteristics.text: Hbulbosum nuclei
characteristics.characteristic: project name
characteristics.text: Anthers with mature pollen grains of 20 ��� 30 H. bulbosum FB19-011-3 florets were collected and the corresponding nuclei were isolated by applying the filter bursting method. The resulting nuclei suspension was stained with propidium iodide (50 ��g/ml, PI) and analyzed on a BD Influx (BD Biosciences) by blotting the PI fluorescence against the side scatter signal (SSC). The nuclei population was selected and a corresponding sorting gate defined in a histogram displaying the fluorescence intensity. Single haploid pollen nuclei were sorted into individual wells of a 96 well plate using the ���1.0 Drop Single��� sort mode of the BD FACS Sortware (BD Biosciences).
characteristics.characteristic: sample description
characteristics.text: IPK_LIMS:2497505
characteristics.characteristic: sample id
characteristics.text: 2021-04-30
characteristics.characteristic: sequencing date ipk
characteristics.text: bulbosum
characteristics.characteristic: species
characteristics.text: not applicable
characteristics.characteristic: year of release