characteristics.text: ERS22058352
characteristics.characteristic: SRA accession
characteristics.text: not collected
characteristics.characteristic: accession name
characteristics.text: A42
characteristics.characteristic: biological material ID
characteristics.text: 112m
characteristics.characteristic: biological material altitude
characteristics.text: 2000m
characteristics.characteristic: biological material coordinates uncertainty
characteristics.text: Germany
characteristics.characteristic: biological material geographic location
characteristics.text: 51.816
characteristics.characteristic: biological material latitude
characteristics.text: 11.283
characteristics.characteristic: biological material longitude
characteristics.text: tetraploid
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PATO_0001382
characteristics.characteristic: biological material ploidy
characteristics.text: not applicable
characteristics.characteristic: biological material preprocessing
characteristics.text: BSDC00002
characteristics.characteristic: checklist
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: Hordeum
characteristics.characteristic: genus
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: greenhouse
characteristics.characteristic: growth facility
characteristics.text: RNAseq
characteristics.characteristic: library type
characteristics.text: not collected
characteristics.characteristic: material source altitude
characteristics.text: not collected
characteristics.characteristic: material source description
characteristics.text: not collected
characteristics.characteristic: material source geographic location
characteristics.text: not collected
characteristics.characteristic: material source latitude
characteristics.text: not collected
characteristics.characteristic: material source longitude
characteristics.text: Hordeum bulbosum
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_4516
characteristics.characteristic: organism
characteristics.text: stem
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/PO_0009047
characteristics.characteristic: plant anatomical entity
characteristics.text: not collected
characteristics.characteristic: plant structure development stage
characteristics.text: Hbulbosum pangenome
characteristics.characteristic: project name
characteristics.text: IPK_LIMS:3310823
characteristics.characteristic: sample ID
characteristics.text: Clones of individuals FB19-011-3, GRA2256, A17, A40 and A42 were grown in a greenhouse at the Leibniz Institute of Plant Genetics and Crop Plant Research. Tissue samples of root, bulb, stem, leaf, stalk, flag leaf and young inflorescensces (including flowers) were harvested , frozen in liquid nitrogen and stored at -80  degree C. Leaf tissues were obtained at the end of the night to reduce the number of photosynthesis-related transcripts in the extracted RNA. Total RNA was isolated with the RNeasy Plant Mini kit (Qiagen, Germany) following the instructions provided by the manufacturer.
characteristics.characteristic: sample description
characteristics.text: 2024-05-31
characteristics.characteristic: sequencing date IPK
characteristics.text: bulbosum
characteristics.characteristic: species