SAMD01615834
External Id
Nihon BioData Corporation
INSDC center name
2025-09-27T00:00:00Z
INSDC first public
2025-09-26T15:28:50Z
INSDC last update
DRS521469
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
DRS521469
SRA accession
Passage 26, 2 days in culture
age
CHL-YN
cell_line
2022-11-30
collection_date
Chinese hamster lung (CHL)-YN cells (RIKEN Bioresource Cell Bank, RCB5004), serum-free suspension, 2 days post-seeding, antibody-producing, scRNA-Seq 76 cells analyzed
description
Japan:Osaka
geo_loc_name
Viable cell density: 2.76 x 10^6 cells/mL, Viability: 98.6%
growth_condition
CHL-YN cells were cultured in 20 mL of EX-CELL CD CHO Fusion (MERCK, 14365C-1000M) supplemented with 600 uL of L-Glutamine (200 mmol/L stock; final concentration, 6.0 mM; FUJIFILM Wako, 073-05391) and 10 uL of Puromycin (10 mg/mL stock; final concentration, 5 ug/mL; InvivoGen, ant-pr-1) in a 125 mL flask. The flask was shaken (140 rpm) at 37 degree C in a humidified 5% CO2 atmosphere (80% relative humidity).
growth_protocol
Cricetulus griseus
http://purl.obolibrary.org/obo/NCBITaxon_10029
organism
KK1902_066_01_YN_pool
sample_name
not applicable
sex
KK1902_066_01_YN_pool
single_cell_identifier
CHL-YN
strain
lung
tissue
KK1902_066_01_YN_pool
title