ERS27956785
SRA accession
110 days
age
20/16 degree Celsius
air temperature regimen
QNC_13
biological material ID
112m
biological material altitude
2000m
biological material coordinates uncertainty
Germany
biological material geographic location
51.816
biological material latitude
11.283
biological material longitude
tetraploid
http://purl.obolibrary.org/obo/PATO_0001382
biological material ploidy
ERC000011
checklist
NaCL
chemical compound
2023-01-29
collection date
0 mM
dose
Titicaca
genotype
Chenopodium
http://purl.obolibrary.org/obo/NCBITaxon_3558
genus
not collected
geographic location (country and/or sea)
greenhouse
http://purl.obolibrary.org/obo/AGRO_00000363
growth facility
50 g sterilized AMF inoculum
infect
RNA-Seq
library type
16h light/8h dark
light regimen
not collected
material source altitude
not collected
material source description
not collected
material source geographic location
not collected
material source latitude
not collected
material source longitude
Chenopodium quinoa
http://purl.obolibrary.org/obo/NCBITaxon_63459
organism
fourth and fifth rows
organism part
vascular leaf post-expansion stage
http://purl.obolibrary.org/obo/PO_0001053
plant anatomical entity
flower meristem transition stage
 http://purl.obolibrary.org/obo/PO_0025588
plant structure development stage
RNAseq_Quinoa_AMF_SaltStress_Leaves_Zaidi_Hajirezaei_2025
project name
rep1
replicate
IPK_LIMS:3418684
sample ID
Quinoa seeds (cv. Titicaca) were surface sterilized by 96% ethanol with 5% (v/v) sodium hypochlorite, and germinated for two weeks in a sterilized standard substrate (Substrate 1, Klasmann-Deilmann GmbH, Germany). Seedlings underwent a three-week vernalization period under controlled conditions: 10 h of light and 14 h of darkness, at 5°C with 80% relative humidity. Mycorrhizal spores were isolated from a palm grove in the Tafilalet region of southeastern Morocco. Following morphological identification, a total of 15 species were identified: Acaulospora delicata, Acaulospora laevis, Acaulospora sp., Claroideoglomus claroideum, Glomus aggregatum, G. claroides, G. clarum, G. deserticola, G. heterosporum, G. macrocarpum, G. microcarpum, Glomus sp., G. versiforme, Rhizophagus intraradices, and Pacispora boliviana (Benaffari et al., 2022). The mycorrhizal inoculum was obtained by cultivating these AMF isolates in the roots of Zea mays L. for three months in sandy soil. This soil, which contained infected corn root fragments, mycelia, and spores, with a spore density of 386 spores per 10 g and a colonization frequency of 80% was collected and used as AMF inoculum. After germination and vernalization, the resulting seedlings were transplanted into 1.5 kg pots. The non-mycorrhizal plants (control) received 50 g sterilized AMF inoculum. The substrate used for this experiment consisted of a nutrient-free substrate (Einheitserde Typ 0, H. Nitsch & Sohn GmbH, Germany) and substrate 1 mixed in a 1:6 ratio, resulting in a total phosphorus content of 50 mg kg-1. The pots were placed in a greenhouse under the following conditions: a 16 h light phase at 20°C and an 8 h dark phase at 16°C. They were arranged in a fully randomized design, with positions changed weekly. The plants were watered daily with distilled water and were fertilized once a week for 16 days using WUXAL® Top N fertilizer (Wilhelms GmbH, Germany). Subsequently, the non-mycorrhizal plants (control) were 11 days watered with 0 mM sodium chloride (NaCl) for non-saline conditions (control). Then leaf material from the fourth and fifth rows in three replicates were collected, immediately frozen in liquid nitrogen, and stored at 80°C for subsequent RNA-seq analyses. Total RNA was extracted with the innuPREP Plant RNA Kit (Analytik Jena, Germany) following manufacturer's instructions.  Library construction and sequencing were done at Novogene (UK) Company Limited. Briefly, RNA integrity and quantitation were determined with the Agilent 2100 Bioanalyzer. Total mRNA was enriched with oligo(dT) beads and randomly fragmented with fragmentation buffer. A stranded specific library was then prepared for each sample and sequenced with a NovaSeq 6000 PE150 platform.
sample_description
QNC_13
sample_title
quinoa cv Titicaca
species
11 days
time
QNC_13
title