2025-11-27
ENA first public
ERC000011
ENA-CHECKLIST
KIS
INSDC center name
public
INSDC status
ERS22541168
SRA accession
E-MTAB-14662:Sample 6
Submitter Id
6
month
age
ArrayExpress
broker name
not collected
collection date
house mouse
common name
Protocols: Freshly isolated small intestinal crypts were cultured as organoids in 60% matrigel for 48 hours, with or without recombinant asporin. Organoids were collected from matrigel and total RNA was extracted from them. Total RNA was isolated using RNeasy Mini Kit (Qiagen) according to the Manufacturer's instructions with on-column DNAse digestion. An Ovation Universal RNA-seq System kit was used for Illumina library preparations (NuGEN Technologies Inc., CA, USA), with rRNA removal according to the kit manual. Libraries were purified with AMPure XP beads.
description
adult
developmental stage
wild type genotype
genotype
not collected
geographic location (country and/or sea)
3
individual
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
epithelium of small intestine
organism part
Mus musculus
scientific_name
female
sex
C57BL/6J
strain
Sample 6
title