2025-11-29
ENA first public
ERC000011
ENA-CHECKLIST
NTNU
INSDC center name
public
INSDC status
ERS27132033
SRA accession
E-MTAB-15858:N79
Submitter Id
ArrayExpress
broker name
not collected
collection date
Atlantic halibut
common name
Protocols: Atlantic halibut eggs were obtained from broodstock at Nordic Halibut AS (Midsund, Norway). Good-quality egg batches from four females were fertilized using cryopreserved milt and incubated in 250 L seawater incubators at 6 °C and 34 ppt salinity. Eggs were sampled at defined developmental stages (unfertilized, 8-cell, 16-cell, blastula, 25 % epiboly, 50 dd), placed in cryovials, snap frozen in liquid nitrogen, and stored at −80 °C until RNA extraction Total RNA was extracted from pools of 10 eggs per sample using the RNeasy Universal Plus Mini Kit (Qiagen) following the manufacturer’s instructions. RNA concentration was measured with a NanoDrop spectrophotometer, and RNA integrity (RIN) was assessed on an Agilent 2100 Bioanalyzer, yielding mean RIN = 9.8 ± 0.24 mRNA libraries were prepared by Novogene Co. (Beijing, China) using their standard Illumina TruSeq RNA Sample Preparation protocol, following manufacturer guidelines for paired-end sequencing. Libraries were quality-checked by agarose gel electrophoresis and Bioanalyzer QC prior to sequencing
description
25% epiboly
developmental stage
not collected
geographic location (country and/or sea)
not applicable
isolate
Hippoglossus hippoglossus
organism
Hippoglossus hippoglossus
organism
whole organism
organism part
Hippoglossus hippoglossus
scientific_name
N79
title