2025-11-30
ENA first public
ERC000011
ENA-CHECKLIST
ITQB NOVA
INSDC center name
public
INSDC status
ERS26728239
SRA accession
E-MTAB-15524:GmaL1
Submitter Id
ArrayExpress
broker name
not collected
collection date
soybean
common name
Williams 82
cultivar
Protocols: At the three fully expanded leaf developmental stage, leaf and root tissues were harvested separately, immediately frozen in liquid nitrogen, and stored at -80°C until processing.  Plants were cultivated in 0.5 L pots containing growth medium (50% soil, 25% vermiculite, 25% peat) in controlled environment chambers at 22°C with a 12/12-hour photoperiod. Total RNA was extracted from approximately 100 mg of tissue using the GeneJET Plant RNA Purification Mini Kit (Thermo Scientific, Vilnius, Lithuania) according to manufacturer's protocols, with three biological replicates per tissue and species. mRNA library preparation was performed using poly A enrichment.
description
LP.03 three leaves visible stage
developmental stage
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Glycine max
organism
Glycine max
organism
leaf
organism part
1
replicate
Glycine max
scientific_name
GmaL1
title