2025-11-30
ENA first public
ERC000011
ENA-CHECKLIST
Folkehelseinstituttet
INSDC center name
public
INSDC status
ERS27144295
SRA accession
E-MTAB-15879:Sample 12
Submitter Id
70
hour
age
ArrayExpress
broker name
not collected
collection date
Protocols: whole worms from petri dishes, Males were separated from hermaphrodites using 40-µm nylon filters and washed three times with M9 buffer Total RNA was extracted from 70-h post-L1 males (him-8 strain; ≥ 300 animals per biological replicate, three biological replicates per condition) subjected to irradiation (0, 1, 10, and 100 mGy/h), using Direct-zol RNA MicroPrep Kit (Zymo Research, Irvine, CA, USA ) according to the manufacturer’s instructions. Males were separated from hermaphrodites using 40-µm nylon filters and washed three times with M9 buffer. The volume was reduced to 150 µL in RNase-free Eppendorf tubes, followed by the addition of 600 µL of Tri Reagent (Zymo Research). Samples were kept on ice before and between homogenization steps using bead beating (⌀ 0.1-0.5 mm) on a FastPrep instrument (10 m/s for 20 seconds, repeated once). Following centrifugation, the supernatant was transferred to new RNase-free tubes, mixed with 750 µL of absolute ethanol, and applied to Zymo-spin IC Columns. On-column DNase I treatment (included in the kit) was performed to remove DNA prior to further purification. RNA concentration (>100 ng/µL), purity (A260/A280 > 1.8; A260/A230 > 2.0), and integrity (RIN > 7) were assessed using Qubit (Thermo Scientific, Waltham, MA, USA), Nanodrop (Thermo Scientific) and Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA), respectively. Library preparation was performed by the Norwegian Sequencing Center (Oslo, Norway) using TruSeqTM stranded RNA-seq paired-end libraries with an average insert size of 350 bp for each biological replicate.
description
larval post-L1
developmental stage
not collected
geographic location (country and/or sea)
pool of >300 individuals
individual
sham
irradiate
not applicable
isolate
Caenorhabditis elegans
organism
Caenorhabditis elegans
organism
whole organism
organism part
Caenorhabditis elegans
scientific_name
male
sex
him-8
strain
Sample 12
title