characteristics.text: 2025-12-01
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: Vanderbilt University
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS23968660
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-15011:11679_S1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: ACBR1 376
characteristics.characteristic: cell line
characteristics.text: brain endothelial cell
characteristics.characteristic: cell type
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Following static or FSS culture, devices were removed from perfusion. Media was aspirated, cells were kept on GEL substrates and washed with PBS and immediately incubated with TRIzol for RNA extraction. Primary human BMECs were purchased from Cell Systems (ACBRI 376) and maintained in Endothelial Cell Growth Media (C-22110, Sigma) up to passage 9. GEL substrates were coated with 50 ug/mL human collagen IV (C5533, Sigma) and 25 ug/mL bovine fibronectin (F1141, Sigma) for 24 hours before cell subculture. BMECs were subcultured on coated GELs either in glass plates (static condition) or in ibidi devices (u-Slide I Luer 3D, ibidi) for the FSS condition. All cells were subcultured at a density of 1e6 cells/mL and allowed to adhere for 24 hours before beginning FSS experiments. BMECs in ibidi devices were subjected to 1.7 dyne/cm2 FSS for 48 hours (following initial 24 hours of flow conditioning at 0.2 dyne) in endothelial cell media supplemented with 3% (w/v) 70kDa dextran and 1X penicillin-streptomycin. Static cultures were maintained in glass plates for 48 hours, with identical media supplements. At the end of each experiment, cells were lysed with TRIzol and RNA was extracted using a Direct-zol RNA miniprep kit (R2050, Zymo). Purified RNA samples were submitted to the Vanderbilt Technologies for Advanced Genomics (VANTAGE) core for bulk sequencing. cDNA libraries were prepared with a stranded mRNA (polyA-selected) library preparation kit.
characteristics.characteristic: description
characteristics.text: 0 dyne/cm2
characteristics.characteristic: fluid shear stress
characteristics.text: 6 kPa
characteristics.characteristic: gel substrate
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: brain blood vessel
characteristics.characteristic: organism part
characteristics.text: 1
characteristics.characteristic: replicate
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: 11679_S1
characteristics.characteristic: title