characteristics.text: 2025-12-01
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: Chang Gung Memorial Hospital
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS25425864
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-15463:WT_37_3
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: THP-1
characteristics.characteristic: cell line
characteristics.text: monocyte
characteristics.characteristic: cell type
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: THP-1 cell lines (ZNF334-wild-type, ZNF334+/−, and ZNF334−/−) were seeded onto 24-well imaging plates (Greiner). For cold stimulation assays, cells were exposed to 32°C for 8 hours in the incubator. Total RNA was isolated from cultured cells using the Qiagen Total RNA Kit according to manufacturer’s instructions. Library were constructed by the Qubit fluorometer with Qubit RNA BR/HS Assay Kit and sample integrity was analyzed by assay method: Agilent 4150 TapeStation system with RNA/High Sensitivity RNA ScreenTape.
characteristics.characteristic: description
characteristics.text: acute monocytic leukemia
characteristics.characteristic: disease
characteristics.text: ZNF334 +/+
characteristics.characteristic: genotype
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: blood
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: 37
characteristics.characteristic: stimulus
characteristics.text: WT_37_3
characteristics.characteristic: title