characteristics.text: 2025-12-01
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: IP
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS25320514
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-15406:WT_pos3
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: glioblastoma U251
characteristics.characteristic: cell line
characteristics.text: stem cell
characteristics.characteristic: cell type
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: GSC (+) have received astrocytic mitochondria (mito-dsRED+) and without astrocytic mitochondria (mito-dsRED-) were sorted after 4 days of co-culture in 2D-culture with murine AS expressing mito-dsRED in a ratio of 3 D to 1 Acc (GSC +), to allow the accumulation of mitochondria, from three independent experiments.  For the extraction, we use the RNeasy microkit Qiagen cDNA libraries were prepared using Illumina Stranded mRNA library Preparation Kit (Illumina) following the manufacturer’s protocol from 250 ng of RNA. Pooled libraries were additionally purified of unbound adaptors and primer dimers on AMPure XP magnetic beads (Beckman-Coulter).
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: cancer-related condition
characteristics.characteristic: disease
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: brain
characteristics.characteristic: organism part
characteristics.text: GSC mitochondria positive
characteristics.characteristic: phenotype
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: WT_pos3
characteristics.characteristic: title