characteristics.text: 2025-12-01
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: Klaus Tschira Institute for Integrative Computational Cardiology, Heidelberg, Germany and DZHK (German Center for Cardiovascular Research), Partner Site Heidelberg/Mannheim, Germany
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS20926284
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-14401:Sample 8
characteristics.characteristic: Submitter Id
characteristics.text: 13
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Cardiomyocyte-specific nuclei isolation was performed via a fluorescence labeling of PCM1 and fluorescence-activated nuclei sorting. PCM1-positive nuclei were subjected to RNA extraction based on the RNeasy Micro Kit (Qiagen). Animals received Tamoxifen for KO induction (75mg/kg BW/d) followed by an Isoprenaline treatment (30mg/kg BW/d) PCM1-positive nuclei were subjected to RNA extraction based on the RNeasy Micro Kit (Qiagen). Library construction was performed according to the SmartSeq2 protocol (Picelli, S., Faridani, O., Björklund, Å. et al. Full-length RNA-seq from single cells using Smart-seq2. Nat Protoc9, 171–181 (2014). https://doi.org/10.1038/nprot.2014.006 and Bianca P Hennig, Lars Velten, Ines Racke, Chelsea Szu Tu, Matthias Thoms, Vladimir Rybin, Hüseyin Besir, Kim Remans, Lars M Steinmetz, Large-Scale Low-Cost NGS Library Preparation Using a Robust Tn5 Purification and Tagmentation Protocol, G3 Genes|Genomes|Genetics, Volume 8, Issue 1, 1 January 2018, Pages 79–89, https://doi.org/10.1534/g3.117.300257)
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: Cre+ Prmt5 wildtype (Ctrl.)
characteristics.characteristic: genotype
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: 4492
characteristics.characteristic: individual
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: heart left ventricle
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: C57BL/6N
characteristics.characteristic: strain
characteristics.text: Sample 8
characteristics.characteristic: title