characteristics.text: 2025-12-01
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: SAMEA114784915
characteristics.characteristic: External Id
characteristics.text: Institute of Molecular Genetics of the Czech Academy of Sciences
characteristics.characteristic: INSDC center name
characteristics.text: 2023-12-01T23:04:05Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS17206027
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-13591:MUT3
characteristics.characteristic: Submitter Id
characteristics.text: 2
characteristics.unit: day
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: Japanese medaka
characteristics.characteristic: common name
characteristics.text: Protocols: WT and Pax6.1-5a-/- knockout embryos collected, incubated to develop into stage 24 and dechorionated. Total RNA was isolated using TRIzolTM Reagent according to TRIzolTM Reagent User Guide. Quality and purity of isolated RNA were determined by 1% of agarose gels. RNA integrity was evaluated via Agilent Bioanalyzer 2100 system using Eukaryote Total RNA Nano and Eukaryote Total RNA Pico assays. The libraries were prepared using the Illumina TruSeq Stranded Total RNA kit in the automation system Biomek FxP.
characteristics.characteristic: description
characteristics.text: embryo stage
characteristics.characteristic: developmental stage
characteristics.text: Pax6.1-5a-/-
characteristics.characteristic: genotype
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Oryzias latipes
characteristics.characteristic: organism
characteristics.text: Oryzias latipes
characteristics.characteristic: organism
characteristics.text: whole organism
characteristics.characteristic: organism part
characteristics.text: Oryzias latipes
characteristics.characteristic: scientific_name
characteristics.text: not available
characteristics.characteristic: sex
characteristics.text: iCab
characteristics.characteristic: strain
characteristics.text: MUT3
characteristics.characteristic: title