2025-12-02
ENA first public
ERC000011
ENA-CHECKLIST
IPSiM, Univ Montpellier, CNRS, INRAE, Institut Agro
INSDC center name
public
INSDC status
ERS23999399
SRA accession
E-MTAB-15039:A14
Submitter Id
8
day
age
ArrayExpress
broker name
not collected
collection date
thale cress
common name
Col0
cultivar
Protocols: 11-day-old seedlings were harvested individually in liquid nitrogen Col0 WT plants were grown on petri dishes after seed sterilisation and stratification, on a solid media containing 1mM KH2PO4, 1mM MgSO4, 0.25mM K2SO4, 0.25mM CaCl2, 0.1mM Na-Fe-EDTA, 2.5mM MES and microelements (50μM KCl, 30μM H3BO3, 5μM MnSO4, 1μM ZnSO4, 1μM CuSO4 and 0.1μM (NH4)6Mo7O24) and 1mM KNO3. A total of 4 plates per genotype and condition were prepared, with 30 seeds per plate (disposed over 2 rows). After 6 days of growth on long days at 22 degrees Celsius and a light intensity of 150 μmol m-2 s-1, seedlings that managed to do the heterotrophic-to-autotrophic transition and continued to grow were transferred to a new plate. RNA was extracted from 11-day-old individual seedlings using the MagMAX™-96 Total RNA Isolation Kit (Ambion) following manufacturer’s instructions except for two changes: 60µl of Lysis/Binding solution were used instead of 100µl, and the elution was performed with 30µl of Elution Buffer instead of 50µl.  Library for sequencing was performed by the novogene company on total RNA using a polyA enrichment method.
description
seedling development stage
developmental stage
wild type genotype
genotype
not collected
geographic location (country and/or sea)
low nitrate conditions (1mM KNO3)
growth condition
not applicable
isolate
Arabidopsis thaliana
organism
Arabidopsis thaliana
organism
whole plant
organism part
Arabidopsis thaliana
scientific_name
seedling 14
seedling
A14
title