2025-12-02
ENA first public
ERC000011:1.3
ENA-CHECKLIST
IES
INSDC center name
public
INSDC status
ERS27131707
SRA accession
EpiLCs_siCTRL
Submitter Id
2025-07-13
collection date
house mouse
common name
2C::tbGFP-PEST mESC E14 were cultured in mESC culture media with Lif and 2i. Media was changed for a media without Lif and 2i for 24, and then changed to EpiLC media (DMEM/F12 supplemented with 20% knockout serum replacement, 2 mM L-glutamine, 1% penicillin/streptomycin, 0.1 mM 2-mercaptoethanol, 0.1 mM nonessential amino acids, 30 ng/ml FGF2, and 0.6 ?M JAKi) for 48h before sample collection. Cells were reverse-transfected at the same time as the media was changed to EpiLC media with siCTRL (from siTools).
description
Germany
geographic location (country and/or sea)
Mus musculus
organism
Mus musculus
scientific_name
EpiLCs_siCTRL
title