2025-12-02
ENA first public
ERC000011
ENA-CHECKLIST
Institute of Life Sciences, Nalco Square, Chandrasekharpur, Bhubaneswar, Odisha
INSDC center name
public
INSDC status
ERS28168884
SRA accession
E-MTAB-12267_2:6_150224_BC6BK7ANXX_P1881_1004
Submitter Id
36
year
age
ArrayExpress
broker name
HL-60
cell line
not collected
collection date
human
common name
Protocols: Leukemic cell line HL-60 was procured from ATCC and maintained in IMDM supplemented with 20% FBS. HL60 cells were cultured in IMDM media supplemented with 20% FBS. HL60 WT cells were induced with 10µM ATRA (All Trans Retinoic Acid) and 50nM Vit-D3 (Vitamin D3) for 72 hours. Total RNA was extracted using Qiagen RNeasy kit using manufacturer’s protocol. Quality and concentration of extracted RNA was checked using Qubit BR RNA kit and Agilent screentape respectively.   Library was constructed using Trueseq stranded sample preparation kit from Illumina using Ribo-zero.
description
adult
developmental stage
acute promyelocytic leukemia
disease
European
ethnic group
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
blood
organism part
Homo sapiens
scientific_name
female
sex
ATRA Induction
stimulus
6_150224_BC6BK7ANXX_P1881_1004
title