2025-12-03
ENA first public
ERC000011
ENA-CHECKLIST
DBT - Institute of Life Science, Bhubaneswar
INSDC center name
public
INSDC status
ERS28165084
SRA accession
E-MTAB-10636:Control
Submitter Id
ArrayExpress
broker name
peripheral blood mononuclear cell
cell type
not collected
collection date
human
common name
Protocols: PBMCs were extracted from 5ml of blood by density gradient centrifugation with the help of Lymphoprep. PBMCs were stained with surface markers CD3 FITC and CD19 PB. Cells were sorted in FACS melody. The sorted single cell suspension was converted to single-cell gel beads in the emulsion (GEMs) by using the Chromium Single Cell 5′ Library and Gel Bead kit, and Chromium Single Cell G Chip kit (10x Genomics) according to the manufacturer’s protocol. scRNA-seq libraries were constructed using a Single Cell 5′ Library and Gel Bead kit, Single Cell V(D)J Enrichment kit, Human T Cell and a Single Cell V(D)J Enrichment kit, Human B Cell according to manufacturer's protocol. The control, contact and patient sample groups contain 3 individuals each which were tagged using TotalSeq™-C antibodies (C0251, C0252, C0253) respectively.
description
adult
developmental stage
normal
disease
not collected
geographic location (country and/or sea)
C1,C2,C3
individual
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
blood
organism part
Homo sapiens
scientific_name
male
sex
Control
title