2025-12-05
ENA first public
ERC000011
ENA-CHECKLIST
Department of Pediatrics, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
INSDC center name
public
INSDC status
ERS27311227
SRA accession
E-MTAB-16154:6491-3_Fgf2-vs-CBD-CNP_Fgf2_R
Submitter Id
ArrayExpress
broker name
not collected
collection date
house mouse
common name
Protocols: Three fresh frozen tibiae per group were OCT embedded, and 10μm frozen sections were cut with cryostat. The tibiae from embryonic day 16.5 mice were cultured in a 24-well plate. The tibiae were divided into two groups (n = 3 per group): one treated with FGF2 alone and the other with FGF2 plus CBD-CNP group. In the FGF2 group, 100 ng/mL FGF2 was administered unilaterally. In the CBD-CNP group, one side received 100 ng/mL FGF2, whereas the opposite site was treated with 100 ng/mL FGF2 and 0.2 µM CBD-CNP for four days with daily medium changes. Nucleic acid was extracted using the RNeasy Mini Kit (Qiagen) according to the manufacturer's instructions. Spatial transcriptome sequencing libraries were prepared with the Visium Spatial Gene Expression Slide & Reagent Kit (10x Genomics) according to the manufacturer's instructions.
description
embryonic day 16.5
developmental stage
not collected
geographic location (country and/or sea)
Fgf2-vs-CBD-CNP
individual
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
right tibia
organism part
Mus musculus
scientific_name
6491-3_Fgf2-vs-CBD-CNP_Fgf2_R
title