2025-12-05
ENA first public
ERC000011
ENA-CHECKLIST
Reader-F (Assistant Professor) National Centre for Biological Sciences, Tata Institute of Fundamental Research
INSDC center name
public
INSDC status
ERS24146991
SRA accession
E-MTAB-15091:VS_AX_C_S71
Submitter Id
ArrayExpress
broker name
not collected
collection date
Protocols: Infected thalli were collected and snap-frozen in liquid nitrogen.   Gemmae from the Mp Tak-1 were plated on Gamborg B5 media without sucrose, solidified with 1.5% agar, and maintained at 22°C under a 20:4-hour day-night photoperiod. For infection, 21-day-old thalli (≥ nine thalli per treatment) were dipped in microconidial suspension for 20 mins. Dipping in water was performed as a mock/control treatment. After air drying for 15 minutes, the infected thalli were incubated at 28°C with a 20:4-hour day-night cycle and samples were harvested at the defined time points.   Fo isolates were grown in Potato Dextrose Broth (PDB) for 72 hours at 28°C and 140 rpm. Microconidia were harvested by filtering fungal cultures through the cheese cloth. The spores were pelleted by centrifugation and resuspended in sterile water.Spore suspension for infection was created by diluting the spore suspension to a final concentration of 10^5 spores/mL. After crushing the tissue in liquid nitrogen, RNA was extracted using Qiagen RNeasy Kit using 100 mg tissue and 500 μL of lysis buffer (washing and elution were done as per the manufacturer’s guidelines). RNA was eluted in 40 μL nuclease water. The extracted RNA was eluted in 40 μL nuclease-free water and quantified using Qubit and quality assessment was performed on Agilent TapeStation. From the total RNA, mRNA was enriched using NEBNext Poly(A) mRNA magnetic isolation Module. The library was prepared using NEBNext Ultra™ II Directional RNA Library Prep with sample purification beads.
description
Axenically grown Fol WT culture
developmental stage
WT Fol 4287
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Fusarium oxysporum
organism
Fusarium oxysporum
organism
Mycelium
organism part
Fusarium oxysporum
scientific_name
VS_AX_C_S71
title
Axenic
treatment