ERC000011
ENA-CHECKLIST
INTERNATIONAL INSTITUTE OF MOLECULAR AND CELL BIOLOGY IN WARSAW
INSDC center name
ERS16372269
SRA accession
DRS_Mex67_AA_10_min_25°C_repB_3_sc
Submitter Id
2022
collection date
brewer's yeast
common name
yeast cells, minimal media that lacked uracil, with 2% raffinose and 2% galactose, Mex67-AA, grow in 25°C, rapamycin added to a final concentration of 1 μg/ml, 10min of treatment, biological replicate B, technical replicate 3. Mixed with 2–5 ug of cap-enriched Arabidopsis thaliana SALK_074069(ABI3MTA), biological replicate 2 mRNA and 50 ng of in vitro-transcribed poly(A) standards. Libraries for nanopore DRS were prepared using SQK-RNA002 sequencing kit (ONT) following the manufacturer’s protocol. Sequencing was performed on the R9 flow cell with the MinION (ONT) device controlled by MinKNOW (ONT) software.
description
Poland
geographic location (country and/or sea)
Saccharomyces cerevisiae
organism
Saccharomyces cerevisiae
scientific_name
DRS_Mex67_AA_10_min_25°C_repB_3_sc. yeast cells, minimal media that lacked uracil, with 2% raffinose and 2% galactose, Mex67-AA, grow in 25°C, rapamycin added to a final concentration of 1 μg/ml, 10min of treatment, biological replicate B, technical replicate 3. Mixed with 2–5 ug of cap-enriched Arabidopsis thaliana SALK_074069(ABI3MTA), biological replicate 2 mRNA and 50 ng of in vitro-transcribed poly(A) standards. Libraries for nanopore DRS were prepared using SQK-RNA002 sequencing kit (ONT) following the manufacturer’s protocol. Sequencing was performed on the R9 flow cell with the MinION (ONT) device controlled by MinKNOW (ONT) software.
title