ERC000011
ENA-CHECKLIST
INTERNATIONAL INSTITUTE OF MOLECULAR AND CELL BIOLOGY IN WARSAW
INSDC center name
ERS16372223
SRA accession
DRS_W303_+_thiolutin_20min_25°C_repA_1_sc
Submitter Id
2022
collection date
brewer's yeast
common name
yeast cells, YPDA, WT W303, grow in 25°C with thiolutin added to a final concentration of 4 ug/ml for 20 minutes, biological replicate A, technical replicate 1. Mixed with 2–5 ug of cap-enriched Arabidopsis thaliana atcfi59-1/atcfi68-3, biological replicate 3, technical replicate 2 mRNA and 50 ng of in vitro-transcribed poly(A) standards. Libraries for nanopore DRS were prepared using SQK-RNA002 sequencing kit (ONT) following the manufacturer’s protocol. Sequencing was performed on the R9 flow cell with the MinION (ONT) device controlled by MinKNOW (ONT) software.
description
Poland
geographic location (country and/or sea)
Saccharomyces cerevisiae
organism
Saccharomyces cerevisiae
scientific_name
DRS_W303_+_thiolutin_20min_25°C_repA_1_sc. yeast cells, YPDA, WT W303, grow in 25°C with thiolutin added to a final concentration of 4 ug/ml for 20 minutes, biological replicate A, technical replicate 1. Mixed with 2–5 ug of cap-enriched Arabidopsis thaliana atcfi59-1/atcfi68-3, biological replicate 3, technical replicate 2 mRNA and 50 ng of in vitro-transcribed poly(A) standards. Libraries for nanopore DRS were prepared using SQK-RNA002 sequencing kit (ONT) following the manufacturer’s protocol. Sequencing was performed on the R9 flow cell with the MinION (ONT) device controlled by MinKNOW (ONT) software.
title