ERC000011
ENA-CHECKLIST
INTERNATIONAL INSTITUTE OF MOLECULAR AND CELL BIOLOGY IN WARSAW
INSDC center name
ERS21044061
SRA accession
FL_E14_T5Cko_42
Submitter Id
2021-11-05
collection date
house mouse
common name
PolyA+ RNA from erythroblasts from fetal liver, biological replicate 2, TENT5C KO mouse in fetal age E14.5. For each biological replicate, 2–2.5 ug of murine cap-enriched RNA mixed with 2–5 ug of cap-enriched Arabidopsis thaliana Col-0 RNA (biological replicate 2, technical replicate 1), 100–150 ng of Saccharomyces cerevisiae oligo(dT)-enriched RNA, and 50 ng of in vitro-transcribed poly(A) standards. Libraries for nanopore DRS were prepared using SQK-RNA002 sequencing kit (ONT) following the manufacturer’s protocol. Sequencing was performed on the R9 flow cell with the MinION (ONT) device controlled by MinKNOW (ONT) software.
description
Poland
geographic location (country and/or sea)
Mus musculus
organism
Mus musculus
scientific_name
PolyA+ RNA from erythroblasts from fetal liver, biological replicate 2, TENT5C KO mouse in fetal age E14.5. For each biological replicate, 2–2.5 ug of murine cap-enriched RNA mixed with 2–5 ug of cap-enriched Arabidopsis thaliana Col-0 RNA (biological replicate 2, technical replicate 1), 100–150 ng of Saccharomyces cerevisiae oligo(dT)-enriched RNA, and 50 ng of in vitro-transcribed poly(A) standards. Libraries for nanopore DRS were prepared using SQK-RNA002 sequencing kit (ONT) following the manufacturer’s protocol. Sequencing was performed on the R9 flow cell with the MinION (ONT) device controlled by MinKNOW (ONT) software.
title