2025-12-10
ENA first public
ERC000011
ENA-CHECKLIST
The Sixth Affiliated Hospital of Xinjiang Medical University
INSDC center name
public
INSDC status
ERS27226698
SRA accession
E-MTAB-16153:OS_4
Submitter Id
ArrayExpress
broker name
not collected
collection date
Norway rat
common name
Protocols: In this study, five femoral tissue samples were collected from the chronic osteomyelitis model group and the control group each, resulting in a total of ten samples. Total RNA extraction using Total RNA extraction Kit (DP431, TIANGEN, China). The quality and quantity of the purified RNA were determined by measuring the absorbance at 260nm/280nm(A260/A280) using Ultrafine spectrophotometer (N50 touch, IMPLEN, Germany). The integrity of RNA was further verified by 1.0% agarose gel electrophoresis For each sample, 500 ng of total RNA was used for cDNA synthesis via a strand-switching reverse transcription approach. The resulting cDNA was then amplified by PCR, followed by barcoding with the Native Barcode kit (SQK-NBD114.96) and purification using Ampure XP Beads. Finally, 300 fmol of the resulting library was loaded onto a PromethION flow cell (vR10.4.4) for sequencing.
description
osteomyelitis
disease
not collected
geographic location (country and/or sea)
Staphylococcus aureus
infect
not applicable
isolate
Rattus norvegicus
organism
Rattus norvegicus
organism
femoral tissue
organism part
Rattus norvegicus
scientific_name
OS_4
title