2025-12-10
ENA first public
ERC000011
ENA-CHECKLIST
Salk
INSDC center name
public
INSDC status
ERS27259869
SRA accession
E-MTAB-16143:Sample 7
Submitter Id
6-10
week
age
ArrayExpress
broker name
Treg
cell type
not collected
collection date
house mouse
common name
Protocols: Samples were sorted into Trizol reagent, frozen at -80, and sent to Genewiz/Azenta Life Sciences for Ultra-low input RNA-seq. Total RNA was extracted from cells following the Trizol Reagent User Guide (Thermo Fisher Scientific). Extracted RNA samples were quantified using Qubit 2.0 Fluorometer (Life Technologies, Carlsbad, CA, USA) and RNA integrity was checked using Agilent TapeStation 4200 (Agilent Technologies, Palo Alto, CA, USA). The SMARTSeq HT Ultra Low Input Kit was used for full-length cDNA synthesis and amplification (Clontech, Mountain View, CA), and Illumina Nextera XT library was used for sequencing library preparation.
description
adult
developmental stage
wild type genotype
genotype
not collected
geographic location (country and/or sea)
chow diet
growth condition
AA3
individual
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
small intestine
organism part
Mus musculus
scientific_name
female
sex
C57BL/6
strain
Sample 7
title