2025-12-11
ENA first public
ERC000011
ENA-CHECKLIST
Division of Neuroscience, IRCCS San Raffaele Scientific Institute, 20132 Milan, Italy Center for Omics Sciences, IRCCS San Raffaele Scientific Institute, 20132 Milan, Italy
INSDC center name
public
INSDC status
ERS27370121
SRA accession
E-MTAB-16211:Sample 2
Submitter Id
90
day
age
ArrayExpress
broker name
not collected
collection date
eastern European house mouse
common name
Protocols: For each replicate, 6-7 sciatic nerves and 3-4 spinal cords were pooled by genotype and dissociated. Control (non-transgenic) P90 sciatic nerves (3 independent replicates), SOD1G93A P90 sciatic nerves (3 replicates), Control P120 sciatic nerves (3 independent replicates), SOD1G93A P120 sciatic nerves (3 replicates), Control P90 spinal cords (3 replicates), SOD1G93A P90 spinal cords (3 replicates), Control P120 spinal cords (4 replicates), SOD1G93A P120 sciatic nerves (4 replicates). An average of 20,000 ECs were purified by FACS for each replicats after staining with rat anti-CD31 APC (1:50; BD Pharmingen Cat#551262) and rat anti-CD45.2 V405 (1:250; BD Biosciences Cat# 560697).  ECs (CD31+/CD45−) were sorted with BD FACSAria Fusion flow cytometer into 400l of Trizol-LS (Thermo Fisher Scientific Cat # 10296010). After quantification and quality control with Qubit 3.0 Fluorometer (Thermo Fisher Scientific) and Agilent 2100 Bioanalyzer, RNA-seq libraries were generated using the SMART-Seq Ultra Low Input RNA Kit (Illumina) (single-end, 100bp read length).
description
adult
developmental stage
WT
genotype
not collected
geographic location (country and/or sea)
NA
individual
not applicable
isolate
Mus musculus musculus
organism
Mus musculus musculus
organism
sciatic_nerve
organism part
Mus musculus musculus
scientific_name
pooled male-female samples
sex
C57Bl6/N
strain
Sample 2
title