2025-12-12
ENA first public
ERC000011
ENA-CHECKLIST
INSERM U1109 HOPITAL CIVIL  Institut d'Hématologie et d'Immunologie 1, Place de l'Hôpital 67091 Strasbourg Cedex
INSDC center name
public
INSDC status
ERS28238679
SRA accession
E-MTAB-16360:Sample 3
Submitter Id
12
week
age
ArrayExpress
broker name
not collected
collection date
house mouse
common name
Protocols: 4-NQO was administered to 8 weeks old WT and TNCKO mice, which had been backcrossed with C57BL/6J mice for over 10 generations. The compound was delivered through drinking water at a final concentration of 100 μg/ml for 16 weeks. Following this treatment, the mice received a single 2 Gy dose of photon irradiation under anesthesia . After irradiation, the animals were provided with regular water for 6 weeks prior to sacrifice. At the time of euthanasia, tongue and mandibular lymph nodes (Tumor draining Lymph Nodes (TdLNs) were harvested.  Total RNA was extracted from lymph node samples using the Qiagen RNeasy Mini Kit according to the manufacturer’s instructions. RNA quality and concentration were assessed using a NanoDrop spectrophotometer and Agilent Bioanalyzer 2100. Only samples with RNA Integrity Number (RIN) ≥ 8 were used for library preparation and downstream RNA-seq analysis. RNA-seq libraries were prepared using NEBNext rRNA Depletion Kit v2 (Human/Mouse/Rat) and NEBNext Ultra II Directional RNA Library Prep Kit Illumina kits.
description
head and neck carcinoma
disease
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
lymph node
organism part
Mus musculus
scientific_name
C57BL/6J
strain
Sample 3
title