2025-12-12
ENA first public
ERC000036:1.3
ENA-CHECKLIST
UGent
INSDC center name
public
INSDC status
ERS27259713
SRA accession
L5
Submitter Id
2023-04-16
collection date
1L raw hospital sewage was collected at UZ Ghent in April, 2023. The sample was stored at 4�C until further processing. Initial clarification was performed by centrifugation at 3 000 � g for 5 min at 4 �C, followed by a second centrifugation of the supernatant at 17,000 � g for 3 min at 4 �C. The resulting clear supernatant was filtered through a 0.45 �m polyethersulfone membrane. Virions were concentrated by polyethylene glycol (PEG) precipitation: NaCl was added to a final concentration of 1 M and dissolved with magnetic stirring, followed by centrifugation at 3 200 � g. PEG 8000 was then added to the supernatant to a final concentration of 10 % (w/v), and samples were incubated on ice for 16 h. Precipitated material was recovered by centrifugation at either 7 196 � g for 40 min or 12 000�17 000 � g for 30 min, both at 4?�C. Pellets were resuspended in 1� DNase buffer (Thermo Scientific) for downstream processing.
description
Belgium
geographic location (country and/or sea)
metagenome
investigation type
Universitair Ziekenhuis Gent Universitair (Corneel Heymanslaan 10, 9000 Ghent, Belgium)
name of the sampling site
viral metagenome
organism
viral metagenome
scientific_name
open sewer line
sewage type
Viral-enriched metagenomic DNA from sewage sample
title