2025-12-13
ENA first public
ERC000011
ENA-CHECKLIST
VIB-UGent Inflammation Research Center
INSDC center name
public
INSDC status
ERS27212875
SRA accession
E-MTAB-16047:AM_IRF4_IL33_rep2
Submitter Id
ArrayExpress
broker name
not collected
collection date
house mouse
common name
Protocols: Alveolar macrophages were collected by bronchoalveolar lavage (BAL). BAL was lightly fixed with formaldehyde (final concentration of 0.1%) quenched with glycine and single-cell suspension was stained with monoclonal antibodies followed by cell sorting. Additional information can be found in original publication. CUT and RUN was carried out with minor adaptations from the protocol  described in the following reference:  \"Skene, P. J., Henikoff, J. G. & Henikoff, S. Targeted in situ genome-wide profiling with high efficiency for low cell numbers. Nat Protoc 13, 1006–1019 (2018)..\"  Additional information can be found in original publication. Libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina according to the manufacturer’s instructions. Cleanup of libraries was performed with AMPure XP beads (Beckman Coulter).
description
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
lung
organism part
Mus musculus
scientific_name
female
sex
IL33
stimulus
C57BL6J
strain
AM_IRF4_IL33_rep2
title