2025-12-13
ENA first public
ERC000011
ENA-CHECKLIST
VIB-UGent Inflammation Research Center
INSDC center name
public
INSDC status
ERS27212882
SRA accession
E-MTAB-16048:AM_ATAC_IL33_rep2
Submitter Id
ArrayExpress
broker name
alveolar macrophage
cell type
not collected
collection date
house mouse
common name
Protocols: Alveolar macrophages were collected by bronchoalveolar lavage (BAL). Single-cell suspension was stained with monoclonal antibodies followed by cell sorting. Additional information can be found in original publication. ATAC-seq was carried out with minor adaptations from the protocol  described in the following reference:  \"Buenrostro, J. D., Wu, B., Chang, H. Y. & Greenleaf, W. J. ATAC-seq: A method for assaying chromatin accessibility genome-wide. Curr Protoc Mol Biol 2015, 21.29.1-21.29.9 (2015).\"  Additional information can be found in original publication. Transposed DNA was amplified using a NEBNext High Fidelity 2× PCR Master Mix (New England Biolabs) with indexed primers. Libraries were purified using SPRIselect beads (Beckman Coulter) to remove primer dimers.
description
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
lung
organism part
Mus musculus
scientific_name
female
sex
IL-33 (3x)
stimulus
C57BL6J
strain
AM_ATAC_IL33_rep2
title