2025-12-13
ENA first public
ERC000011
ENA-CHECKLIST
VIB-UGent Inflammation Research Center
INSDC center name
public
INSDC status
ERS27141194
SRA accession
E-MTAB-15952:Exp1_Irf4-/- addback_IL33_4
Submitter Id
ArrayExpress
broker name
not collected
collection date
house mouse
common name
Protocols: Experiment 1:  WT or IRF4-/- alveolar macrophages (AMs) were culture expanded and transferred into depleted SPAM-deleter animals. After 1 week, all mice were subjected to IL-33 induced inflammation (3 consecutive doses of 100 ng intratracheally). AMs were harvested from bronchoalveolar lavage fluid 1 day after the final dose of IL-33 and FACS sorted into RLTplus buffer, supplemented with 1% βmercaptoethalol. Qiagen RNeasy Plos Micro kit following manufacturer's instructions Illumina stranded total RNA library prep kit was used following manufacturer's instruction
description
Irf4 -/-
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
Alveolar macrophage
organism part
Mus musculus
scientific_name
female
sex
3 x IL-33 intratracheal
stimulus
SPAM-deleter
strain
Exp1_Irf4-/- addback_IL33_4
title