2025-12-13
ENA first public
ERC000011
ENA-CHECKLIST
VIB-UGent Inflammation Research Center
INSDC center name
public
INSDC status
ERS27141187
SRA accession
E-MTAB-15952:Exp1_IL33_1
Submitter Id
ArrayExpress
broker name
not collected
collection date
house mouse
common name
Protocols: Experiment 1:  WT or IRF4-/- alveolar macrophages (AMs) were culture expanded and transferred into depleted SPAM-deleter animals. After 1 week, all mice were subjected to IL-33 induced inflammation (3 consecutive doses of 100 ng intratracheally). AMs were harvested from bronchoalveolar lavage fluid 1 day after the final dose of IL-33 and FACS sorted into RLTplus buffer, supplemented with 1% βmercaptoethalol. Qiagen RNeasy Plos Micro kit following manufacturer's instructions Illumina stranded total RNA library prep kit was used following manufacturer's instruction
description
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
Alveolar macrophage
organism part
Mus musculus
scientific_name
female
sex
3 x IL-33 intratracheal
stimulus
C57BL6J
strain
Exp1_IL33_1
title