2025-12-14
ENA first public
ERC000011
ENA-CHECKLIST
School of Pharmaceutical Sciences, Southern Medical University
INSDC center name
public
INSDC status
ERS27426997
SRA accession
E-MTAB-16261:M2
Submitter Id
8
week
age
ArrayExpress
broker name
not collected
collection date
Norway rat
common name
Protocols: Kidney cortex (~30 mg) was dissected from 8-week-old male Sprague–Dawley rats under isoflurane anesthesia, immediately snap-frozen in liquid nitrogen, and stored at –80 °C until DNA extraction. Genomic DNA was extracted using the DNeasy Blood & Tissue Kit (Qiagen) following the manufacturer’s instructions. DNA was quantified with Qubit dsDNA BR and checked for integrity (agarose gel ≥20 kb, 260/280 ≥1.8) DNA ( ~ 350 bp) was fragmented, end-repaired, A-tailed, and ligated to bisulfite-compatible adaptors, followed by bisulfite conversion. Sequencing libraries were constructed and quality-checked, then sequenced.
description
adult
developmental stage
tubulointerstitial fibrosis
disease
wild type genotype
genotype
not collected
geographic location (country and/or sea)
M2
individual
not applicable
isolate
Rattus norvegicus
organism
Rattus norvegicus
organism
kidney
organism part
Rattus norvegicus
scientific_name
male
sex
Sprague-Dawley
strain
M2
title