2025-12-14
ENA first public
ERC000011
ENA-CHECKLIST
Maastricht University Department of Translational Genomics
INSDC center name
public
INSDC status
ERS27185360
SRA accession
E-MTAB-16038:TB208_UNTR_REP7
Submitter Id
ArrayExpress
broker name
shGFP
cell line
cardiomyocyte
cell type
not collected
collection date
human
common name
Protocols: After the exposure, cells were washed once with PBS and subsequently lysed in 0.5 mL QIAzol Lysis Reagent (79306, Qiagen). Total RNA was extracted using chloroform and isopropanol precipitation. 50 ng of total RNA isolated from SV40 immortalized cardiomyocytes samples were used as input for the NEXTFLEX® Combo-Seq™ mRNA/miRNA Kit (NOVA-5139-53, PerkinElmer). All RIN (RNA Integrity Number) values were 8 or higher. We performed 18 PCR cycles were performed during library preparation. RNA-Seq libraries were quantified on a Qubit 2.0 Fluorometer (ThermoFisher) and quality control checked on the 2200 TapeStation System (Agilent).
description
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
Homo sapiens
scientific_name
TB208_UNTR_REP7
title