1
Block
2025-12-14
ENA first public
ERC000022
ENA-CHECKLIST
SAMEA115029621
External Id
INRA-CMSE
INSDC center name
2023-12-14T10:47:30Z
INSDC last update
public
INSDC status
ERS17476457
SRA accession
EFELE_2015_03_PRO_108_18S
Submitter Id
0N
Treatment
terrestrial environmental zone
broad-scale environmental context
2015
collection date
0.2
m
depth
The present work monitored, over a decade, the cumulative effect of repeated digestate application on soil microbial communities employing and in-situ approach, comparing the effect of the digested feedstock to the same undigested feedstock, and to others fertilization treatments. The study was carried out at the experimental field site EFELE, located in Rennes, France. The experiment was set up in 2012 and is based on a wheat-maize crop rotation. A mineral fertilizer (MIN), and three different OWP: cattle manure (CM), pig slurry (PS), and pig slurry anaerobic digestate (PS-DIG), were applied once every year since the beginning of the trial to the present. These treatments were compared to a control (0N) that had not received any organic or mineral N input. To monitor soil microbial community dynamics, from 2012 to 2022, soil samples were collected every year twelve months after the last fertilization treatment. For the molecular analyses performed in this study, were selected soil samples belonging to the beginning of the trial (2012), and wheat crop years (2013, 2015, 2017, 2019, and 2022). All the molecular analyses were conducted in 2022; we used high-throughput sequencing targeting 16S and 18S ribosomal RNA genes to describe the dynamics of microbial soil communities. Our results highlighted that over a decade of annual inputs of OWPs or mineral fertilizer, the soil chemical properties, the microbial biomass, and the composition and structure of soil microbial communities followed lasting modifications in all evaluated treatments. These changes were primarily explained by the time effect and, to a lesser extent, by the quality of the applied product. The temporal factor (year) exerted a strong influence on all measured parameters, potentially reflecting environmental, climatic, and seasonal effects that could mask the impact of digestate on soil microorganisms. Moreover, soil management practices appear to buffer the effects of different treatments, whether organic or mineral, on soil microbial quality indicators. The impact of digestate on soil microbial communities should be assessed considering associated agricultural practices.
description
34
m
elevation
agricultural soil
environmental medium
France
geographic location (country and/or sea)
48.102
DD
geographic location (latitude)
-1.7956
DD
geographic location (longitude)
area of cropland
local environmental context
F: 5'-CGTACAGTCA-3'; R: 5'-TGCTCTATAC-3'
multiplex identifiers
soil metagenome
organism
F: 5'-CGATAACGAACGAGACCT-3'; R: 5'-ANCCATTCAATCGGTANT-3'
pcr primers
PRJEB71034
project name
1
g
sample volume or weight for DNA extraction
soil metagenome
scientific_name
18S
target gene
Dynamics of microbial soil communities over 10 years of repeated organic and inorganic fertilization
title