2025-12-15
ENA first public
ERC000011
ENA-CHECKLIST
ED
INSDC center name
public
INSDC status
ERS23781177
SRA accession
E-MTAB-14914:C3H.Brain.MA.33a.1700.B.1M
Submitter Id
7 to 11
week
age
ArrayExpress
broker name
not collected
collection date
house mouse
common name
Protocols: The mouse samples sequenced in this study were part of a large mutation accumulation (MA) experiment described in previous studies (see Publications).  All tissue and RNA extractions were made from male mice with similar ages, ranging from 7 to 11 weeks old. Tissue extractions were always carried out at similar times during the day (10am-12am Central European Time) at the facilities of the Max Planck Institute. The whole brain was used for RNA extraction, and liver samples always came from the same liver lobe. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers. Then the second strand cDNA was synthesized using dUTP, instead of dTTP. The directional library was ready after end repair, A-tailing, adapter ligation, size selection, USER enzyme digestion, amplification, and purification
description
adult
developmental stage
wild type genotype
genotype
not collected
geographic location (country and/or sea)
C3H.33a.1700.B.1M
individual
not applicable
isolate
33
line number
MA
line type
Mus musculus
organism
Mus musculus
organism
brain
organism part
Mus musculus
scientific_name
male
sex
C3H/HeNRj
strain
C3H.Brain.MA.33a.1700.B.1M
title