characteristics.text: 2025-12-19
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: PhD student, Department of Pathology, University of Cambridge, UK
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS28318822
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-16365:Sample 7
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: PBMCs
characteristics.characteristic: cell line
characteristics.text: natural killer cell
characteristics.characteristic: cell type
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Human PBMC-derived NK cells were cultured with or without cortisol (1 µM) and, in parallel, co-cultured with K562 target cells for 6 hours. At the end of treatment, NK cells were sorted to high purity by flow cytometry, collected directly into RNase-free tubes, and immediately processed for RNA extraction. Total RNA was extracted from sorted NK cells using the Qiagen RNA extraction kit following the manufacturer’s instructions (including on-column DNase digestion when applicable). RNA quantity and purity were assessed using NanoDrop spectrophotometry. High-quality RNA (RIN ≥ 7) was used for downstream library preparation. RNA-seq libraries were generated by Novogene using their standard mRNA-seq workflow. Briefly, poly-A–enriched mRNA was isolated, fragmented, and reverse-transcribed to cDNA, followed by end repair, A-tailing, adaptor ligation, and PCR amplification. Libraries were purified, quantified, and assessed for size distribution prior to sequencing.
characteristics.characteristic: description
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: blood
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.characteristic: stimulus
characteristics.text: Sample 7
characteristics.characteristic: title