characteristics.text: 2025-12-19
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: PhD student, Department of Pathology, University of Cambridge, UK
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS28322683
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-16367:Sample 2
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: NK-92
characteristics.characteristic: cell line
characteristics.text: natural killer cell
characteristics.characteristic: cell type
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: CAR-NK-92, and NR3C1-knockout CAR-NK-92 cells were cultured under defined experimental conditions. Cells were treated with hydrocortisone and/or CoCl₂ and subsequently co-cultured with A549 target cells for 6 hours. Following co-culture, NK-92 cells were collected, washed in cold PBS, and pelleted in RNase-free tubes. Cell pellets were processed immediately for RNA extraction. Total RNA was extracted from sorted NK cells using the Qiagen RNA extraction kit following the manufacturer’s instructions (including on-column DNase digestion when applicable). RNA quantity and purity were assessed using NanoDrop spectrophotometry. High-quality RNA (RIN ≥ 7) was used for downstream library preparation. RNA-seq libraries were sequenced by Novogene using an Illumina platform, generating paired-end reads. Standard Illumina sequencing chemistry and quality control procedures were applied. Raw FASTQ files were delivered after base calling, filtering, and adapter trimming performed by Novogene.
characteristics.characteristic: description
characteristics.characteristic: genetic deletion
characteristics.text: CEACAM5 specific CAR expression
characteristics.characteristic: genetic modification
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: blood
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.unit: micromolar
characteristics.characteristic: steroid treatment
characteristics.text: Cocultured with CEACAM5 expressing A549 cell line
characteristics.characteristic: stimulus
characteristics.text: Sample 2
characteristics.characteristic: title