characteristics.text: 2025-12-19
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: PhD Student, Department of Pathology, University of Cambridge, UK
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS28326385
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-16381:Sample 5
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Tumors were excised from LLC-OVA tumor–bearing C57BL/6J mice on day 19. Each tumor was mechanically minced and enzymatically digested using IMDM supplemented with 10% FBS, collagenase D (1 mg/mL), collagenase A (1 mg/mL), and DNase I (0.4 mg/mL). Digestion was performed at 37 °C for 30–40 minutes with gentle agitation. Collagenase activity was terminated by adding EDTA (5 mM) to the samples. The resulting suspension was filtered through 70 µm cell strainers to obtain a single-cell suspension. Tumor-infiltrating NK cells were isolated by flow cytometric sorting based on CD3⁻ CD19⁻ CD56⁺ immunophenotype and collected in RNase-free tubes for downstream processing. Total RNA was extracted from sorted NK cells using the Qiagen RNA extraction kit according to the manufacturer’s instructions, including optional on-column DNase digestion. RNA concentration and purity were determined using a NanoDrop spectrophotometer, and RNA integrity was assessed using Bioanalyzer. Only high-quality RNA samples were used for library construction. RNA-seq libraries were prepared by Novogene using their standard mRNA-seq library preparation pipeline.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Subcutaneous tumor
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: C57BL/6J
characteristics.characteristic: strain
characteristics.text: Sample 5
characteristics.characteristic: title