characteristics.text: 2025-12-19
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: PhD student, department of Pathology, University of Cambridge, UK
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS28322653
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-16373:Sample 17
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: NK-92
characteristics.characteristic: cell line
characteristics.text: natural killer cell
characteristics.characteristic: cell type
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: NK-92, CAR NK-92, hydrocortisone-treated CAR NK-92, NR3C1-knockout CAR NK-92, and hydrocortisone-treated NR3C1-knockout CAR NK-92 cells were co-cultured with CEACAM5⁺ A549 target cells for 16 hours. After co-culture, NK-92–derived cells were collected by FACS sorting. Sorted cell pellets were immediately processed for RNA extraction. Total RNA was extracted using the Qiagen RNA extraction kit following the manufacturer’s instructions, including optional on-column DNase digestion. RNA concentration and purity were assessed using NanoDrop. High-quality RNA was used for subsequent library preparation by Novogene.  RNA-seq libraries were generated by Novogene using their standard mRNA-seq workflow.
characteristics.characteristic: description
characteristics.text: CEACAM5 specific CAR expression and NR3C1 KO
characteristics.characteristic: genetic modification
characteristics.text: NR3C1 KO CEACAM5-CAR
characteristics.characteristic: genotype
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: Cocultured with CEACAM5 expressing A549 cells
characteristics.characteristic: growth condition
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: Sample 17
characteristics.characteristic: title