characteristics.text: 2025-12-20
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: Amsterdam University Medical Centres,  department of Psychiatry
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS28164330
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-16319:Sample 99
characteristics.characteristic: Submitter Id
characteristics.text: 2
characteristics.unit: day
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Mice designated for fresh culls were sacrificed by decapitation between 10 am and 12 pm. Brain regions were rapidly dissected on ice. Crude isolation of the whole PFC and surrounding cortical regions was performed by first removing the olfactory bulbs. Briefly, a coronal cut was made at the anterior extent of the brain to define the dissection boundary (approx. Bregma 2.93-1.69 mm) The ventral portion of this section (olfactory bulb, nucleus accumbens, piriform cortex, and other subcortical regions), was carefully discarded. The remaining dorsal cortical tissue, encompassing the prefrontal cortex, orbital cortex, and adjacent cortical areas, was isolated for subsequent analyses. This approach aimed to capture a broad region of the prefrontal cortex relevant to our study while minimizing contamination from ventral and subcortical structures. Notably, this would have included both grey and white matter tissue. All biological samples were promptly snap-frozen on dry ice and stored at -80°C until further analysis. Briefly, a coronal cut was made at the anterior extent of the brain to define the dissection boundary (approx. Bregma 2.93-1.69 mm) The ventral portion of this section (olfactory bulb, nucleus accumbens, piriform cortex, and other subcortical regions), was carefully discarded. The remaining dorsal cortical tissue, encompassing the prefrontal cortex, orbital cortex, and adjacent cortical areas, was isolated for subsequent analyses. This approach aimed to capture a broad region of the prefrontal cortex relevant to our study while minimizing contamination from ventral and subcortical structures. Notably, this would have included both grey and white matter tissue.  RNA was extracted from the PFC using the RNeasy Plus Universal Mini Kit (Qiagen), according to the manufacturer’s instructions. RNA quality and concentrations were then measured using a Nanodrop ND-1000 (Thermo Scientific).  Library preparation was performed by Azenta. Poly(A) enriched, strand specific.
characteristics.characteristic: description
characteristics.text: mouse postnatal
characteristics.characteristic: developmental stage
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: GERM_FREE
characteristics.characteristic: growth condition
characteristics.text: 59_21
characteristics.characteristic: individual
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: prefrontal cortex
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: M
characteristics.characteristic: sex
characteristics.text: C57/BL6
characteristics.characteristic: strain
characteristics.text: Sample 99
characteristics.characteristic: title