characteristics.text: replicate 1: tcacgctgtacaaggtgaagcagacc, replicate 2: gaccactgtacaaggtgaagcagacc, replicate 3: ccaatctgtacaaggtgaagcagacc
characteristics.characteristic: Barcoded primers ON-target Stoplight sequence
characteristics.text: replicate: 1 tcacgCTCTTGCTGACTCGACTGGT, replicate 2: gaccaCTCTTGCTGACTCGACTGGT, replicate 3: ccaatCTCTTGCTGACTCGACTGGT
characteristics.characteristic: Barcoded primers Off-target 1 (chr2:+120580702)
characteristics.text: replicate 1: tcacgGTGCAGGGAAAACACCATAGG, replicate 2: gaccaGTGCAGGGAAAACACCATAGG, replicate 3: ccaatGTGCAGGGAAAACACCATAGG
characteristics.characteristic: Barcoded primers Off-target 2 (chr4:157825214)
characteristics.text: replicate 1: tcacgGGCATCTTGAACTTGGGCAT, replicate 2: gaccaGGCATCTTGAACTTGGGCAT, replicate 3: ccaatGGCATCTTGAACTTGGGCAT
characteristics.characteristic: Barcoded primers Off-target 3 (chr14:104942109)
characteristics.text: replicate 1: tcacgCTCGCAGTCTCGCACCAC, replicate 2: gaccaCTCGCAGTCTCGCACCAC, replicate 3: ccaatCTCGCAGTCTCGCACCAC
characteristics.characteristic: Barcoded primers Off-target 4 (chr5:180630776)
characteristics.text: replicate 1: tcacgTGACTCTCTTGGTTACAGGGG, replicate 2: gaccaTGACTCTCTTGGTTACAGGGG, replicate 3: ccaatTGACTCTCTTGGTTACAGGGG
characteristics.characteristic: Barcoded primers Off-target 5 (chr8:135581932)
characteristics.text: SAMN52827038
characteristics.characteristic: External Id
characteristics.text: Utrecht University
characteristics.characteristic: INSDC center name
characteristics.text: 2025-10-16T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2025-10-16T17:38:32.583Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS26864126
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Human
characteristics.characteristic: NCBI submission model
characteristics.text: Human.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS26864126
characteristics.characteristic: SRA accession
characteristics.text: passage 53
characteristics.characteristic: age
characteristics.text: Olivier de Jong, Pharmaceutics, UU, Universiteitsweg 99, 3584 CG, Utrecht, The Netherlands
characteristics.characteristic: biomaterial_provider
characteristics.text: HEK293T
characteristics.characteristic: cell_line
characteristics.text: 2025-03-04
characteristics.characteristic: collection_date
characteristics.text: ATCC:3216
characteristics.characteristic: culture_collection
characteristics.text: Amplicon NGS
characteristics.characteristic: description
characteristics.text: Netherlands:Utrecht
characteristics.characteristic: geo_loc_name
characteristics.text: DNA from HEK293T cells transfected with pDNA expressing Cas9 and a sgRNA targeting a fluorescent Stoplight reporter construct
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Cell culture
characteristics.characteristic: sample_type
characteristics.text: female
characteristics.characteristic: sex
characteristics.text: Cell line
characteristics.characteristic: tissue
characteristics.text: Cas9 SL1 targeting transfection
characteristics.characteristic: title
characteristics.text: Cas9 transfection
characteristics.characteristic: treatment