characteristics.text: 2025-12-21
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: ACTREC - Tata Memorial Centre
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS20530037
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-14224:PS1 Relapse YBX3sh1 replicate 1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: Patient derived culture - 1
characteristics.characteristic: cell_line
characteristics.text: GBM
characteristics.characteristic: cell_type
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Cells were grown to 60% confluence in 10-cm culture dishes, media was removed, and RNA-Isoplus Reagent (Takara Bio) was added to the culture dishes and kept for 2 min on ice for complete cell lysis.  The suspension was collected in a microcentrifuge tube, and RNA was extracted by adding chloroform and precipitating it with isopropanol. RNA samples were quantitated on a Qubit fluorometer, and appropriate dilutions were loaded onto a high-sensitivity RNA screen tape to determine the RNA integrity number (RIN). Samples with 28S/18S peak integrated area ratios of >2 were used for library preparation. 1.5 μg of QC-verified RNA samples was used for poly (A) mRNA isolation from total RNA using the NEB Next Poly (A) mRNA Kit (Cat. no. NEB# 7490), according to the manufacturer’s protocol. The NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (cat. no. E7760L) was used to construct double-stranded cDNA libraries from the polyA RNA fraction using RNA fragmentation, first-strand cDNA synthesis with random primers, second-strand cDNA synthesis, end repair of double-stranded cDNA, adapter ligation, removal of excess adapter using sample purification beads, PCR enrichment of adapter-ligated DNA, and clean-up of PCR products as instructed by the manufacturer. The cleaned libraries were quantitated on a Qubit fluorometer, and appropriate dilutions were loaded on a high-sensitivity D1000 screen tape to determine the size range of the fragments and average library size.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: glioblastoma multiforme
characteristics.characteristic: disease
characteristics.text: Relapsed
characteristics.characteristic: genotype
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: brain
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: PS1 Relapse YBX3sh1 replicate 1
characteristics.characteristic: title