characteristics.text: SAMD00787177
characteristics.characteristic: External Id
characteristics.text: Osafune Laboratory, Kyoto University, Center for iPS Cell Research and Application (CiRA)
characteristics.characteristic: INSDC center name
characteristics.text: 2025-11-10T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2025-11-11T14:49:39.420Z
characteristics.characteristic: INSDC last update
characteristics.text: DRS533829
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Human
characteristics.characteristic: NCBI submission model
characteristics.text: Human.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: DRS533829
characteristics.characteristic: SRA accession
characteristics.text: not applicable
characteristics.characteristic: age
characteristics.text: Shinya Yamanaka
characteristics.characteristic: biomaterial_provider
characteristics.text: hiPS cell derived nephron progenitor cells
characteristics.characteristic: cell_type
characteristics.text: 2021-06-29
characteristics.characteristic: collection_date
characteristics.text: We seeded the human nephron progenitor cell (NPC)s into 96-well low cell-binding U-bottom plates at a density of 2.0 x 10^4 cells/well with serum-free differentiation medium consisting of DMEM/F12 Glutamax (Thermo Fisher Scientific), B27 supplement minus vitamin A (Thermo Fisher Scientific) and 0.5X Penicillin/streptomycin (PS) containing 1 uM CHIR99021 (Wako), 200 ng/mL FGF9 (Peprotech) and 10 uM Y-27632 (hereafter called NPC medium) to form aggregates. Every other day, 100 uL of the medium was exchanged. After 168 h, we placed three aggregates per well of 96-well plates with the NPC medium (37 degree C) or HypoThermosol (4 degree C; Charles River Laboratories Cell Solutions, Inc.). We placed the culture plate under ambient atmospheric conditions in incubators set at 4 degree or under 5% CO2 conditions in incubators set at 37 degree. After 48 h incubation, cellular aggregates were washed with PBS (-) and collected. Details about the differentiation of hiPSCs towards NPCs are described elsewhere (Tsujimoto et. al 2020).
characteristics.characteristic: description
characteristics.text: Japan
characteristics.characteristic: geo_loc_name
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: 1231A3_4deg_2
characteristics.characteristic: sample_name
characteristics.text: not applicable
characteristics.characteristic: sex
characteristics.text: not applicable
characteristics.characteristic: tissue
characteristics.text: Nephron progenitor cells, iPSC derived, cooled, rep2
characteristics.characteristic: title