characteristics.text: SAMN52754574
characteristics.characteristic: External Id
characteristics.text: University of Hull
characteristics.characteristic: INSDC center name
characteristics.text: 2025-10-15T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2025-11-17T13:25:31.280Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS27077882
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Metagenome or environmental
characteristics.characteristic: NCBI submission model
characteristics.text: Metagenome.environmental.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS27077882
characteristics.characteristic: SRA accession
characteristics.text: 2023-06
characteristics.characteristic: collection_date
characteristics.text: Environmental DNA (eDNA) collected in June 2023 from water, soil, tree-surface roller swabs, and mammal scat at a 40 ha rewilding site in the Scottish Highlands, United Kingdom. Each of ten Illumina MiSeq libraries (Lib1-Lib10) pools 24 uniquely tagged eDNA samples amplified with vertebrate mitochondrial 12S-V5 primers 12SV5F (5'-TAGAACAGGCTCCTCTAG-3') and 12SV5R (5'-TTAGATACCCCACTATGC-3') following Riaz et al. (2011). DNA was extracted using the Mu-DNA soil protocol (Sellers et al., 2018) and libraries were prepared using the nested two-step PCR workflow described by Griffiths et al. (2023). The raw data deposited here represent pooled amplicon libraries prior to bioinformatic demultiplexing.
characteristics.characteristic: description
characteristics.text: United Kingdom: Inverness
characteristics.characteristic: geo_loc_name
characteristics.text: not applicable
characteristics.characteristic: host
characteristics.text: rewilding site with broadleaf and conifer woodland (water, soil, tree-surface roller, and scat samples)
characteristics.characteristic: isolation_source
characteristics.text: 57.1879 N 4.4883 W
characteristics.characteristic: lat_lon
characteristics.text: soil metagenome
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_410658
characteristics.characteristic: organism
characteristics.text: not applicable
characteristics.characteristic: rel_to_oxygen
characteristics.text: Sterile collection WhirPak bags, sterile spoons for soil and scat, tree-surface roller swabs
characteristics.characteristic: samp_collect_device
characteristics.text: Water and tree samples filtered then frozen, soil and scat samples frozen after collection
characteristics.characteristic: samp_mat_process
characteristics.text: water: 1L; tree: 400ml; soil: 50ml; scat: 50ml
characteristics.characteristic: samp_size
characteristics.text: Lib1; Lib2; Lib3; Lib4; Lib5; Lib6; Lib7; Lib8; Lib9; Lib10
characteristics.characteristic: source_material_id
characteristics.text: Pooled eDNA libraries from four terrestrial substrates at a 40 ha rewilding site, Scottish Highlands (June 2023)
characteristics.characteristic: title