2025-12-23
ENA first public
ERC000011
ENA-CHECKLIST
Klinik und Poliklinik für Innere Medizin II TUM Klinikum rechts der Isar Universität München
INSDC center name
public
INSDC status
ERS28164413
SRA accession
E-MTAB-16320:511418 + 9801 + MycInhibition
Submitter Id
ArrayExpress
broker name
cancer cell line
cell line
not collected
collection date
house mouse
common name
Protocols: Cells were seeded at a density of 10.000 cells in 6 cm dishes and after attachment, were cultured for 24 hours. Culture in standard medium +/- 60 µM 10058-F4 (Myc Inhibitor) Total RNA of cancer cell lines was isolated using Maxwell® 16 LEV simplyRNA Tissue Kit (AS1280, Promega) and Maxwell® 16 Instrument (Promega) as per manufacturer’s instructions Library preparation for bulk 3’-sequencing of poly(A)-RNA was done as described previously (Parekh et al., 2016) barcoded cDNA of each sample was generated with a Maxima RT polymerase (Thermo Fisher) using oligo-dT primer containing barcodes, unique molecular identifiers (UMIs) and an adapter. 5’ ends of the cDNAs were extended by a template switch oligo (TSO) and after pooling of all samples full-length cDNA was amplified with primers binding to the TSO-site and the adapter. cDNA was tagmented with the Nextera XT kit (Illumina) and 3’-end-fragments finally amplified using primers with Illumina P5 and P7 overhangs.
description
pancreatic cancer
disease
KrasG12D
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
pancreas
organism part
Mus musculus
scientific_name
511418 + 9801 + MycInhibition
title