SAMN54223443
External Id
University of Missouri
INSDC center name
2025-12-19T00:00:00Z
INSDC first public
2025-12-19T13:02:26.263Z
INSDC last update
live
INSDC status
Model organism or animal
NCBI submission model
Model.organism.animal.1.0
NCBI submission package
6
Passage number
ATCC
biomaterial_provider
NIH-3T3
cell_line
Embryonic fibroblast
cell_type
ATCC
collected_by
2023-04-13
collection_date
NIH-3T3 cells, a mouse fibroblast cell line, were procured from ATCC. 3T3 cells were cultured in DMEM-F-12 medium supplemented with 10 percent Serum Plus II and maintained at 37 C in a humidified atmosphere containing 5 percent CO2. For experiments, approximately 70,000 cells per well were seeded into 12-well plates, and treatments were initiated once the cells reached 60-70 percent confluence. Due to the high mortality of 3T3 cells in serum-deprived medium, all treatments were performed in 2 percent serum-supplemented medium. This group was not treated with anything and was used as a control.
description
Embryonic
dev_stage
USA
geo_loc_name
mus musculus embryonic fibroblast
isolation_source
Mus musculus
http://purl.obolibrary.org/obo/NCBITaxon_10090
organism
cell culture
sample_type
not applicable
sex
NIH/Swiss
strain
Embryonic fibroblast
tissue
3T3-Control 1
title
no treatment
treatment