2025-12-26
ENA first public
ERC000011
ENA-CHECKLIST
Gannan medical university
INSDC center name
public
INSDC status
ERS28360223
SRA accession
E-MTAB-16423:si_NC_2
Submitter Id
ArrayExpress
broker name
GC-1
cell line
male germ cell
cell type
not collected
collection date
house mouse
common name
Protocols: The cells (mouse GC-1) were maintained in Dulbecco’s Modified Eagle Medium and Nutrient Mixture F-12 (DMEM/F-12; Gibco, USA), supplemented with 10% fetal bovine serum (FBS;ScienCell, USA), and 1% penicillin-streptomycin solution (NCM Biotech, China).  The RNA extracted from the cells was initially subjected to quality control. mRNAs with poly(A) tails were enriched using  oligo(dT) magnetic beads. These mRNAs were then randomly fragmented using divalent cations in a specified buffer, and library construction was performed following the NEB standard library construction protocol.
description
neonate
developmental stage
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
testis
organism part
Mus musculus
scientific_name
si_NC_2
title