2025-12-27
ENA first public
ERC000011
ENA-CHECKLIST
Universidad de León, León, Spain
INSDC center name
public
INSDC status
ERS25359017
SRA accession
E-MTAB-15450:Sample 4
Submitter Id
~25
day
age
ArrayExpress
broker name
not collected
collection date
sheep
common name
Protocols: Samples of liver tissue were collected from the carcasses of the 12 lambs under study at the time of slaughter. These samples were preserved in an RNA-stabilizing solution (Ambion RNAlater; Life Technologies) and kept at 4°C for 24 hours. Afterward, the RNA-stabilizing solution was discarded, and the samples were frozen at −80°C until RNA extraction.  The extraction of RNA from the liver samples was conducted using a standard protocol for RNA extraction with Trizol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's instructions. The RNA integrity value was calculated using the Agilent 2,100 Bioanalyzer equipment (Agilent Technologies, CA, USA), which was higher than 7 for all the samples.  The cDNA library construction  was conducted at Novogene in Cambridge (UK) using the UltraTM RNA Library Prep Kit (NEBNext®, MA, USA).
description
juvenile
developmental stage
not collected
geographic location (country and/or sea)
not applicable
isolate
Ovis aries
organism
Ovis aries
organism
liver
organism part
Ovis aries
scientific_name
female
sex
Sample 4
title