2025-12-30
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA110292013
External Id
Helmholtz Zentrum Munich
INSDC center name
2022-07-08T13:50:31Z
INSDC last update
public
INSDC status
ERS12389621
SRA accession
E-MTAB-11835:Sample 2
Submitter Id
ArrayExpress
broker name
SKM#1
cell_line
induced pluripotent stem cell
cell_type
house mouse
common name
Protocols: The mouse iPSC were a gift from Hans Schöler lab: Velychko, S., Adachi, K., Kim, K.-P., Hou, Y., MacCarthy, C.M., Wu, G., and Schöler, H.R. (2019). Excluding Oct4 from Yamanaka Cocktail Unleashes the Developmental Potential of iPSCs. Cell Stem Cellhttps://doi.org/10.1016/j.stem.2019.10.002.ROSA26-rtTA/GOF18Briefly, mouse embryonic fibroblasts of passage 3 or 4 were reprogrammed by lentiviral infection with a dox inducible cassette expressing Sox2/Klf4/c-Myc. Strain is a mixed background C57BL/6 and 129. Cells were trypsinized and FACS sorted to select live and single cells and to exclude feeder cells colored in red. Cells were washed twice and passed through a cell strainer 35um before final counting and viability assessment with an automatic cell counter. Chromium Singe Cell 3’ v3, 10x Genomics Chromium Singe Cell 3’ v3, 10x Genomics
description
embryo
developmental stage
dox inducible cassette Sox2/Klf4/c-Myc
genotype
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
organism part
fibroblast
progenitor cell type
Mus musculus
scientific_name
C57BL/6 and 129
strain
Sample 2
title