2025-12-30
ENA first public
ERC000011
ENA-CHECKLIST
GMU
INSDC center name
public
INSDC status
ERS26813541
SRA accession
E-MTAB-15575:03050-3
Submitter Id
ArrayExpress
broker name
not collected
collection date
Protocols: The WT, Δxtr, and ::xtr strains were inoculated into BHIS medium and cultured until the OD600 reached 0.6. The cultures were then centrifuged at at 13,500 × g for 10 minutes, the supernatant was removed, and the bacterial pellets of each strain were collected. The samples were transported with adequate dry ice in an foam box to Nanjing Personalomics Co., Ltd. for transcriptome sequencin Total RNA was extracted from WT, Δxtr, and ::xtr using a bacterial total RNA extraction kit (DP430, TIANGEN, Beijing). Once the samples were deemed satisfactory, the following steps were conducted for sequencing: (1) Removal of ribosomal RNA; (2) Enrichment and purification of mRNA; (3) Fragmentation of mRNA; (4) Construction of the sequencing library and quality assessment of the library.
description
03050 deletion
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Clostridioides difficile 630
organism
Clostridium difficile 630
organism
Clostridioides difficile 630
scientific_name
03050-3
title