2025-12-30
ENA first public
ERC000011
ENA-CHECKLIST
Wageningen University & Research
INSDC center name
public
INSDC status
ERS24291511
SRA accession
E-MTAB-15101:B2A21
Submitter Id
2
block
ArrayExpress
broker name
not collected
collection date
Protocols: On 10 June 2022, during peak biomass season, we took six soil cores from each of the 48 plots using a 1-cm-diameter soil auger (each 10 cm deep). We did this in all 4 corners (each 30 cm from the edges of the plot) and twice in the center (50 cm from south to north edge and either 40 cm from west or east edge). Soil samples per plot were combined in a plastic bag and stored in a cool box until transported to the laboratory. Samples were sieved using a 2-cm mesh sieve to remove stones, plant material and larger animals. A representative subsample of the sieved soil from each plot was frozen at -20°C for later DNA analyses, the rest was stored at 4°C for soil property analyses. DNA was extracted from 1 g of soil according to Harkes et al. (2025). Sequencing libraries were generated by pooling PCR products of the samples in equimolar ratio’s to ensure even sequencing depth. The libraries were then cleaned using NucleoMag NGS Clean-up and Size Select beads (0.6X bead:sample ratio) to discard unwanted contaminants and short DNA fragments (<600 bp). Of each library, 150 fmol was prepared for nanopore sequencing using kit SQK-LSK112 (Oxford Nanopore Technologies Plc., UK) following instructions of the manufacturer.
description
1
fertilization
not collected
geographic location (country and/or sea)
not applicable
isolate
Experimental field station of the Helmholtz Centre for Environmental Research (UFZ) in Bad Lauchstädt (Saxony-Anhalt, Germany; 51°23′38″ N, 11°52′45″ E, 118 m a.s.l.)”
location
mixed sample
organism
mixed sample
organism
whole organism
organism part
4
plant species richness
soil micro-eukaryota
sample type
mixed sample
scientific_name
B2A21
title