2025-12-30
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA13315453
External Id
INSERM U1242 OSS (Oncogenesis, Stress and Signaling) - Universite de Rennes1
INSDC center name
2022-03-08T16:47:43Z
INSDC last update
public
INSDC status
ERS10918254
SRA accession
E-MTAB-11518:Sample 3
Submitter Id
ArrayExpress
broker name
sperm
cell_type
human
common name
Protocols: Human semen samples were collected from healthy donors of unproven fertility at Nantes University Hospital (France). Total RNA was extracted using the NucleoSpin RNA XS Kit (Macherey Nagel) from 35 µL human spermatozoa cells. Quality control was realized on the RNA extracts with a 2100 bioanalyzer on chip Pico RNA (Agilent Technologies) to measure RNA Integrity Number then on nanodrop 2000 (Nanodrop) for the quantitative measurement. Reverse transcription and cDNA amplification were performed with WTA2 kit (Sigma Aldrich) from 100 ng of total RNA according to the manufacturer’s instructions. Libraries were prepared from 1 ug cDNA with the NEBNext Ultra II DNA kit and Index kit Set A (Biolabs). The final pool was quantified by Qubit dsDNA High Sensitivity kit (Thermofischer) at 45 nM and qualified with the Bioanalyzer at an average size of 359 bp. To correct the unbalanced base composition of the libraries prepared, we used a PhiX spike-in (15%).
description
adult
developmental stage
normal
disease
P3
individual
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
semen
organism substance
Homo sapiens
scientific_name
male
sex
Sample 3
title