2025-12-31
ENA first public
ERC000011
ENA-CHECKLIST
IRMB - Institute for Regenerative Medicine & Biotherapy - INSERM U1183
INSDC center name
public
INSDC status
ERS20522319
SRA accession
E-MTAB-14217:WT-O-1
Submitter Id
21
month
age
ArrayExpress
broker name
not collected
collection date
house mouse
common name
Protocols: Harvested knees joint were dissected using surgical scissors, snap frozen immediately, and then stored in liquid nitrogen until the extraction step. Protocol for young mice: The induction of osteoarthritis will be performed by injecting 5 μl of type VII collagenase (1U/5μl) into the joint of one of the mouse's knees on day 0 and day 2 (the other knee being injected with physiological saline solution). The mice will be anesthetized with isoflurane, and the leg will be shaved at the knee joint. A small incision (2mm) of the skin will be made at the central part of the joint. The needle of a 10μl Hamilton syringe will be inserted at the center of the patellar tendon to reach the intra-articular space, and the collagenase solution (or the  physiological saline solution for the Sham knee joint) will be injected. The wound is sterilized with betadine. CiOA mice were euthanized at day 42 after the first collagenase injection. Protocol for old mice: Osteoarthritis in older mice is spontaneous age-related. Therefore, the mice underwent no interventions. They were simply euthanized at 18 months.  The grinding of the whole joint was performed using a pestle and mortar in liquid nitrogen. Total RNA from the resulting powder tissue was extracted using TRI Reagent (Sigma Aldrich #T94424) and RNeasy Kit (QIAGEN) according to the manufacturer’s instructions. The quantity and quality of extracted RNA was evaluated using the NanoDrop ND-1000 spectrophotometer and the Agilent 2100.  Nucleic acid library construction protocol was performed according to the manufacturer’s instructions by the Beijing Genomic Institute (BGI).
description
adult
developmental stage
p21+/+
genotype
not collected
geographic location (country and/or sea)
WT-old
individual
Old WT
infect
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
knee joint
organism part
Mus musculus
scientific_name
male
sex
C57BL/6N
strain
WT-O-1
title