2025-12-31
ENA first public
ERC000011
ENA-CHECKLIST
Ecole Polytechnique Federale de Lausanne (EPFL)
INSDC center name
public
INSDC status
ERS26752532
SRA accession
E-MTAB-14195_2:Liver_TRT_HFD_NRH_000_M_rep_11
Submitter Id
8
week
age
L-HFD-11
alternate name
C03
barcode
TCCAAGGCATTAAG
barcode sequence
20250716
batch
96 V5D Odt
brbseq barcode kit
ArrayExpress
broker name
not collected
collection date
house mouse
common name
Protocols: Male and Female C57Bl/6NTac were purchased from Taconic. All mice were kept in a standard temperature- and humidity-controlled environment with a 12h:12h light-dark cycle. Mice had nesting material and ad libitum access to water and commercial diets. For the studies in Figures 1-3, mice were fed a regular mouse housing diet (Safe 150). For the high-fat diet study, we used D12492 from Research Diets, while using D12450J as a sucrose matched control. NRH was provided to mice in the drinking water, at a concentration of 0.1% of 0.4%, which approximately leads to a dose of 100 mg/kg*day or 400 mg/kg*day assuming mice drink around 3 ml per day and weight around 30 grams. Water bottles were renewed twice a week with a fresh solution. For the high-fat diet, the dose of NRH in the water bottles was adjusted weekly according to the average body weight of the cage. All animal experiments were carried out according to national Swiss and EU ethical guidelines and approved by the local animal experimentation committee under licenses VD2770.1, VD3647 and VD3841.  Animals were sacrificed after a 2 h fast, in order to stabilize systemic parameters and to allow the measurement of blood biochemistry in the fasting state. Tissues were collected upon sacrifice and flash-frozen in liquid nitrogen. Total mRNAs were extracted as described previously (Ratajczak, J. et al. Nat Commun, 2016) BRB-seq libraries were generated using a MERCURIUS BRB-seq kit (Alithea Genomics, #10813) according to the manufacturers instructions
description
adult
developmental stage
high fat diet
diet
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
20250716_BRBseq_Liver_TRT
library
Mus musculus
organism
Mus musculus
organism
liver
organism part
Mus musculus
scientific_name
male
sex
C57Bl/6NTac
strain
Treatment study
study
Liver
tissue
Liver_TRT_HFD_NRH_000_M_rep_11
title
Control
treatment
Demultiplexed sample
type