2025-12-31
ENA first public
ERC000011
ENA-CHECKLIST
USYD
INSDC center name
public
INSDC status
ERS28366246
SRA accession
E-MTAB-16453:Sample 8
Submitter Id
ArrayExpress
broker name
not collected
collection date
human
common name
Protocols: Premortem cardiac samples were collected from the left ventricle (LV) right ventricle (RV) or right atrium (RA) within 15 minutes via snap freezing in liquid nitrogen Approximately 50mg of tissue/sample was disrupted using a metal bead (Qiagen #69989) in a 2mL tubes with 500uL TRIzol (Ambion #15596018) using the TissueLyser LT (Qiagen) and RNA extraction performed according to TRIzol manufacturer’s instructions. DNA removal was performed using Qiagen RNAse-Free DNase kit (Qiagen #79254) and purified using the RNeasy Mini kit (Qiagen, #74104) according to manufacturer’s instructions. RNA quality was evaluated using a Nanodrop and an RNA Nano Chip (Agilent #5067-1511) on an Agilent Bioanalyzer. Libraries were prepared with Illumina Stranded Total RNA prep Ligation with Ribo Zero Plus kit, according to manufacturer’s instructions. Libraries were sequenced using a paired-end 250bp Illumina NovaSeq 6000 S4 flow cell.  Approximately 50mg of tissue/sample was disrupted using a metal bead (Qiagen #69989) in a 2mL tubes with 500uL TRIzol (Ambion #15596018) using the TissueLyser LT (Qiagen) and RNA extraction performed according to TRIzol manufacturer’s instructions. DNA removal was performed using Qiagen RNAse-Free DNase kit (Qiagen #79254) and purified using the RNeasy Mini kit (Qiagen, #74104) according to manufacturer’s instructions. RNA quality was evaluated using a Nanodrop and an RNA Nano Chip (Agilent #5067-1511) on an Agilent Bioanalyzer. Libraries were prepared with Illumina Stranded Total RNA prep Ligation with Ribo Zero Plus kit, according to manufacturer’s instructions. Libraries were sequenced using a paired-end 250bp Illumina NovaSeq 6000 S4 flow cell.
description
not available
developmental stage
myocardial infarction
disease
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
heart
organism part
MI RA 2
sampling site
Homo sapiens
scientific_name
Sample 8
title