2025-12-31
ENA first public
ERC000011
ENA-CHECKLIST
Laboratory of Traslational Research. Azienda USL-IRCCS di Reggio Emilia
INSDC center name
public
INSDC status
ERS18113505
SRA accession
E-MTAB-13777:MDAt41_sgRUNX2_2
Submitter Id
ArrayExpress
broker name
MDA-T41
cell_line
not collected
collection date
human
common name
Protocols: Cells were collected after 10 days from selection. TPC1 cells were cultured in DMEM medium supplemented with 10% FBS and 1% P/S. MDA-T41 cells were grown in RPMI-1640 supplemented with 1% non-essential amino acids, 10% FBS and 1% penicillin-streptomycin. All cell lines were grown at 37°C/ 5% CO2 and routinely tested for mycoplasma infection. Cells were infected with pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro containing either sgRAIN, sgRUNX2 or sgNT, then selected with 1 µg/ml Puromycin for 3 days. Total RNA was extracted by Maxwell RSC simplyRNA Cells kit (Promega, Madison, Wisconsin, USA) Libraries were obtained starting from 100ng of total RNA following the Illumina Stranded Total RNA preparation Ligation with Ribo-Zero Plus protocol.
description
adult
developmental stage
papillary thyroid carcinoma
disease
RAIN_sgRNA
genetic modification
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
thyroid gland
organism part
2
replicate
Homo sapiens
scientific_name
MDAt41_sgRUNX2_2
title