2025-12-31
ENA first public
ERC000011
ENA-CHECKLIST
Department of Medicine 3 – Rheumatology and Immunology, Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg and Universitätsklinikum Erlangen
INSDC center name
public
INSDC status
ERS24604138
SRA accession
E-MTAB-15134:HTO1aa
Submitter Id
age
ArrayExpress
broker name
not collected
collection date
human
common name
Protocols: Entheseal biopsy of the extensor common tendon´s enthesis was performed. Briefly, biopsies were done by Blakesley forceps in 60° flexed position after identifying the lateral epicondyle, the olecranon and the radial head by ultrasound. Entheseal tissue slides from six patients before and after treatment with secukinumab were analyzed with GeoMX Digital Spatial Profiler (Nanostring) Tissue slides were prepared following the RNA Slide Preparation Protocol in the GeoMx NGS Slide Preparation User Manual (NanoString, MAN¬ 10150-06). Briefly, slides were baked at 60°C for 60 min and then treated sequentially with HIER (ER2, 20 min, 99°C) and 1 mg/mL proteinase K (Ambion, cat. 2546) in 1X phosphate-buffered saline (PBS) at 37°C for 15 min. After pretreatment, slides were hybridized with the GeoMx Whole Transcriptome Atlas Panel (GMX-RNA-NGS-HuWTA-4). The library preparation and purification were carried out using GeoMX DSP NGS Readout Protocol (MAN-10153-06).
description
developmental stage
disease
not collected
geographic location (country and/or sea)
Negative control
individual
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
enthesis
organism part
NA
response to treatment
NA
roi
Homo sapiens
scientific_name
Negative control
segment
sex
1
slide
HTO1aa
title