2025-12-31
ENA first public
ERC000011
ENA-CHECKLIST
nbdeyy
INSDC center name
public
INSDC status
ERS27094925
SRA accession
E-MTAB-15762:BM-LLC
Submitter Id
ArrayExpress
broker name
RAW264.7
cell line
macrophage
cell type
not collected
collection date
house mouse
common name
Protocols: Total RNA was extracted from RAW264.7 cells after 72 hours of exosome treatment using Trizol reagent (Invitrogen, #15596026). RAW264.7 murine macrophage cells were cultured in Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin. Cells were maintained at 37°C in a humidified incubator with 5% CO₂. RAW264.7 cells were treated with 10 μg/mL of exosomes (isolated from NC-LLC or BM-LLC cell culture supernatants) for 72 hours. The culture medium was refreshed every 48 hours. Total RNA was extracted from cells using Trizol reagent (Invitrogen, #15596026) according to the manufacturer's instructions. RNA quality and concentration were assessed using a NanoDrop spectrophotometer. RNA libraries for sequencing were constructed using the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB). Briefly, mRNA was enriched, fragmented, and reverse-transcribed into cDNA. After end repair and adapter ligation, libraries were amplified by PCR. The final library quality was checked using an Agilent Bioanalyzer.
description
normal
disease
not collected
geographic location (country and/or sea)
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
Mus musculus
scientific_name
BM-LLC exosomes treated
stimulus
BM-LLC
title