2025-12-31
ENA first public
ERC000011
ENA-CHECKLIST
AO Research Institute Davos, Davos, Switzerland
INSDC center name
public
INSDC status
ERS20948771
SRA accession
E-MTAB-14418:Sample 43
Submitter Id
8
week
age
ArrayExpress
broker name
not collected
collection date
house mouse
common name
Protocols: RNA was isolated from the epiphysis of one femur per mouse that was homogenized in TRIzol® (n=3 for each of the following mouse genotypes: SHIPstyx/styx, SHIP1+/styx, wild-type (C57BL6/J), Rag2-/-/Il2rg-/-/SHIP1styx/styx, Rag2-/-/Il2rg-/-/SHIP1+styx, and Rag2-/-/Il2rg-/-/SHIP1+/+). Alternatively, total RNA was extracted using the NucleoSpin® RNA Plus Kit . RNA was isolated according to the manufacturer’s instructions and RNA concentration and integrity were assessed using a Thermo Fisher Scientific Qubit 4.0 fluorometer with the Qubit RNA BR Assay Kit (Thermo Fisher Scientific, Q10211) and an Advanced Analytical Fragment Analyzer System using a Fragment Analyzer RNA Kit (Agilent, DNF-471), respectively.  Sequencing libraries were made with 1000 ng input RNA using an illumina TruSeq Stranded mRNA Library Prep kit (illumina, 20020595) in combination with TruSeq RNA UD Indexes (illumina, 20022371) according to illumina’s guidelines.
description
mouse postnatal
developmental stage
SHIP1styx/styx
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
Epiphysis of femur
organism part
Mus musculus
scientific_name
female
sex
C57BL6/J
strain
Sample 43
title