2025-12-31
ENA first public
ERC000011
ENA-CHECKLIST
Research Institute for Reproductive Medicine and Genetic Diseases, Wuxi Maternity and Child Health Care Hospital
INSDC center name
public
INSDC status
ERS22821317
SRA accession
E-MTAB-14740:siNC_3
Submitter Id
ArrayExpress
broker name
SKOV3
cell_line
epithelial cell
cell_type
not collected
collection date
human
common name
Protocols: OC cell lines (SKOV3, A2780, OVCAR3, HO8910 and CaoV3) and a normal ovarian epithelial cell line (IOSE80) were obtained from the Cell Bank of Chinese Academy of Sciences (Shanghai, China), and cultured in a humidified incubator at 37°C with 5% CO2. RPMI-1640 medium (Gibco, USA) supplemented with 1% penicillin/streptomycin (PS) (NCM Biotech, China) and 10% fetal bovine serum (FSP500, ExCell Bio, China) was used to culture IOSE80, SKOV3, A2780,HO8910 and CaoV3 cells. OVCAR3 cells were cultured in RPMI-1640 medium containing 20% fetal bovine serum. SiRNAs (Gene Pharma, Shanghai, China) targeting CDIPTOSP,STAU1, and a negative control (si-NC) were transfected into SKOV3 and A2780 cells using Lipofectamine 2000 (Invitrogen, USA). Total RNA was extracted from cells transfected for 48 hours using TRIzol reagent (Vazyme, Nanjing, China). Subsequently, reverse transcription was performed using the HiScript III RT SuperMix for qPCR kit (Vazyme) in accordance with the manufacturer's instructions.  RNA-Seq on SKOV3 cells was performed by LC-Bio Technology (Hangzhou, China), involving library preparation with poly-A enrichment, cDNA synthesis, and sequencing on an Novaseq™ 6000 platform.
description
adult
developmental stage
ovarian cancer
disease
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
ovary
organism part
Homo sapiens
scientific_name
siNC_3
title