2025-12-31
ENA first public
ERC000011
ENA-CHECKLIST
Tettamanti Center, Fondazione IRCCS San Gerardo dei Tintori, Monza, Italy
INSDC center name
public
INSDC status
ERS21342529
SRA accession
E-MTAB-14549:GEX_225_postInfusion_CD3+
Submitter Id
17
year
age
ArrayExpress
broker name
T cell
cell_type
not collected
collection date
human
common name
Protocols: Frozen human BM and Infusion Product (IP) cryotubes were rapidly thawed and stained with hCD45 PO (clone HI30, Invitrogen) and CD3 PerCP (clone SK7, BD) or with CD19 biotin protein (Miltenyi) followed by Biotin APC (REA746, Miltenyi) and CD3 PercP (clone SK7, BD) antibodies, respectively. BM cells were separated into CD45+CD3+ and CD45+CD3- fractions, whereas IP was separated into CD3+CAR+ and CD3+CAR- cells by flow cytometry-based sorting (BD FACS ARIA III analyser). All samples were gated based on physical parameters, followed by the exclusion of doublets and death cells (DAPI high). After sorting, cells were counted and checked for viability For each sample,cells were loaded in a 10x Genomics Chromium Next GEM Chip according to manusfacturer’s protocol Libraries were constructed using 10x Genomics Chromium Next GEM Single Cell 5’ v1 kit
description
childhood
developmental stage
B-cell acute lymphoblastic leukemia
disease
not collected
geographic location (country and/or sea)
2
individual
Commercial anti-CD19 CAR T
infusion product
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
bone marrow
organism part
Homo sapiens
scientific_name
female
sex
GEX_225_postInfusion_CD3+
title