2025-12-31
ENA first public
ERC000011
ENA-CHECKLIST
Gyeongsang National University Animal Genomics Laboratory
INSDC center name
public
INSDC status
ERS26890337
SRA accession
E-MTAB-15578:BS-b10-8
Submitter Id
12
year
age
ArrayExpress
broker name
not collected
collection date
dog
common name
Protocols: A total of 24 beagle dogs aged 3 years (n = 8), 5 years (n = 8), and 10 years (n = 8) were investigated for age-related molecular signatures in their methylomes. All dogs were kept at the NIAS under standardized housing conditions that were maintained through continuous monitoring, vaccination, and anthelmintic treatment. The experiment was ethically approved by the Institutional Animal Care and Use Committee of the National Institute of Animal Science (NIAS 2022-586) and Gyeongsang National University (GNU-240802-D0152-01). Whole blood samples were collected using a 21-gauge needle and 5-mL syringe from the jugular vein of each dog. DNA was extracted from whole blood using an Exgene Blood SV mini kit (GeneAll Biotechnology, Seoul, Republic of Korea) according to the manufacturer’s protocol.  Libraries were prepared using the xGen Methyl-Seq Library Prep Kit (Integrated DNA Technologies, Coralville, IA, USA). The input DNA concentration was analyzed using Qubit. A total of 100 ng genomic DNA was fragmented to an estimated size of 350 bp using the S2 Focused Ultrasonicator system (Covaris, Woburn, MA, USA).
description
adult
developmental stage
not collected
geographic location (country and/or sea)
not applicable
isolate
Canis lupus familiaris
organism
Canis lupus familiaris
organism
blood
organism part
Canis lupus familiaris
scientific_name
male
sex
BS-b10-8
title