SAMN45145900
External Id
Westlake University, Hangzhou, Zhejiang, China
INSDC center name
2025-12-30T00:50:39.580Z
INSDC first public
2025-12-30T00:50:39.580Z
INSDC last update
SRS23440723
INSDC secondary accession
live
INSDC status
Model organism or animal
NCBI submission model
Model.organism.animal.1.0
NCBI submission package
SRS23440723
SRA accession
72 hours
age
Chun-Qing Song
biomaterial_provider
2024-09
collection_date
China: HangZhou
geo_loc_name
Human 293T cells from ATCC were cultured at 37 C in high-glucose DMEM (HyClone, SH30243.01) supplemented with 10% (vol/vol) FBS (GIBICO, 10099141C) and 1% penicillin-streptomycin (Hyclone, SV30010). Cells were transfected at 8090% confluency using polyethylenimine linear (PEI) (Polyscience, 23966), according to the manufacturer's instructions. The medium was replaced with fresh medium 10-12 h after PEI transfection. For each well of a 24-well plate, cells were transfected with 500 ng plasmid including activation domain, 300 ng dCas9 plasmid and 300 ng sgRNA-2PP7 plasmid. Cells were collected 72 h after transfection, rep1.
isolate
Mus musculus
http://purl.obolibrary.org/obo/NCBITaxon_10090
organism
not determined
sex
293T cell line
tissue
bulkRNAseq_293T_control
title