2025-12-31
ENA first public
ERC000011
ENA-CHECKLIST
UofA
INSDC center name
public
INSDC status
ERS27379145
SRA accession
E-MTAB-16224:Sample 6
Submitter Id
ArrayExpress
broker name
CT29
cell line
trophoblast cell
cell type
not collected
collection date
human
common name
Protocols: CT29 cell lines were grown and treated with either PRKCZ-targetting siRNA or a scrambled control. The cell lines were used to generate placental organoids. CT 29 lines were maintained on 5 µg/mL collagen IV coated plates and using human TSC culture medium in a humidified incubator at 37 degrees and 5% CO2. Organoids were generated using a rotatory cell culture system. CT29 knockdowns were achieved utilizing PRKCZ-targetting siRNA that was transfected into the cell line using Opti-MEM I Reduced Serum Medium and Lipofectamine LTX Reagent with PLUS reagent.  Placental organoids were pelleted and resuspended in trizol before being mechanically dissociated utilizing a tissue lyser. RNA was extracted by utilizing Invitrogen PureLink RNA Mini Kit. Bulk seq libraries were constructed using the Illumina Stranded mRNA Prep Kit.
description
PRKCZ knockdown
genetic modification
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
Homo sapiens
scientific_name
Sample 6
title