2025-12-31
ENA first public
ERC000011
ENA-CHECKLIST
Vanderbilt University
INSDC center name
public
INSDC status
ERS27414133
SRA accession
E-MTAB-16239:5XFAD 1
Submitter Id
12
month
age
ArrayExpress
broker name
endothelial cell
cell type
not collected
collection date
house mouse
common name
Protocols: Brains from male hemizygous 5XFAD (n=3) and wild-type control mice (n=3) aged 12 months were used. Brain tissue (excluding olfactory bulbs and cerebellum) was dissociated into a single cell suspension, and Fluorescence Activated Cell Sorting (FACS) was used to isolate CD31+, CD45- cells Following Fluorescence Activated Cell Sorting (FACS), total RNA was extracted from the isolated CD31+, CD45- brain endothelial cells. Nucleic acid isolation was performed according to the manufacturer's protocol. Single-cell gene expression libraries were generated using the 10x Genomics Chromium Next GEM Single Cell 5'Gene Expression, v2 Chemistry kit. Libraries were prepared according to the manufacturer's protocol, and raw data were later processed using Cell Ranger against the GRCm39-2024-A reference
description
adult
developmental stage
hemizygous
genotype
not collected
geographic location (country and/or sea)
5XFAD 1
individual
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
brain
organism part
Mus musculus
scientific_name
male
sex
5XFAD (C57BL/6J)
strain
5XFAD 1
title