2025-12-31
ENA first public
ERC000011
ENA-CHECKLIST
Fuwai Hospital, CAMS&PUMC
INSDC center name
public
INSDC status
ERS25081636
SRA accession
E-MTAB-15255:C4
Submitter Id
17
week
age
ArrayExpress
broker name
not collected
collection date
house mouse
common name
Protocols: After anesthesia with tribromoethanol, the ventricle was obtained after systemic perfusion with PBS. Tissues of ~20mg were harvested and stored at -80°C. Using the regular TRIzol method. Library was prepared using Optimal Dual-mode mRNA Library Prep Kit (BGI-Shenzhen). Library preparation is performed using Optimal Dual-mode mRNA Library Prep Kit (BGI-Shenzhen, China). A certain amount of RNA are denatured at suitable temperature to open the secondary structure, and mRNA is enriched by oligo (dT) attached magnetic beads. After reacting at a suitable temperature for a fixed time period, RNAs are fragmented with fragmentation reagents.  Then First-strand cDNA is generated using random hexamer-primed reverse transcription, followed by a second-strand cDNA synthesis. The synthesized double strand cDNA is subject to end repairment reaction. After cDNA end repairment, a single ‘A’ nucleotide is added to the 3’ ends of the blunt fragments through A tailing reaction. Then the reaction system for adaptor ligation configured to ligate adaptors with the cDNAs, and finally, the library products are amplified through PCR reaction and subjected to quality control.  Next, the single-stranded library products are produced via denaturation. The reaction system for circularization is set up to get the single-stranded cyclized DNA products. Any uncyclized single stranded linear DNA molecules will be digested.
description
adult
developmental stage
heart failure
disease
Healthy control
disease staging
wild type genotype
genotype
not collected
geographic location (country and/or sea)
C4
individual
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
heart
organism part
Mus musculus
scientific_name
male
sex
C57BL/6N
strain
C4
title