2025-12-31
ENA first public
ERC000011
ENA-CHECKLIST
CyberomiX Inc.
INSDC center name
public
INSDC status
ERS24945261
SRA accession
E-MTAB-15226:Slide2
Submitter Id
77, 72, 77, 51
year
age
ArrayExpress
broker name
not collected
collection date
human
common name
Protocols: HCC samples, including the tumor-non-tumor boundary, were collected from seven patients who underwent hepatic resection at the Department of Surgery, Kyoto University Hospital. Tissue specimens, approximately 10 × 10 mm in size, were excised from the tumor-non-tumor boundary immediately after hepatic resection. The samples were fixed in 10% neutral buffered formalin for 6–48 h. Fixed samples were embedded in formalin-fixed paraffin-embedded (FFPE) blocks and stored under refrigeration until sectioning. 5 µm sections were prepared and processed according to the 10x Genomics Visium CytAssist Spatial Gene Expression for FFPE protocol (CG000520, Rev B). This protocol included deparaffinization, hematoxylin and eosin staining, imaging, and decrosslinking steps. High-resolution histological images were captured with an APX100 microscope (Evident).  Library preparation was subsequently performed according to the Visium CytAssist Spatial Gene Expression Reagent Kit (CG000495, Rev B) for human transcriptomes on slides with 6.5 mm or 11 mm capture areas.
description
adult
developmental stage
hepatocellular carcinoma
disease
not collected
geographic location (country and/or sea)
case1, 2, 4, 7
individual
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
liver
organism part
Homo sapiens
scientific_name
female, male
sex
Slide2
title