2025-12-31
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA114624346
External Id
German Cancer Research Center (DKFZ)
INSDC center name
2023-11-15T20:28:46Z
INSDC last update
public
INSDC status
ERS16999817
SRA accession
E-MTAB-13538:scc25-nf_scc25_01
Submitter Id
ArrayExpress
broker name
scc-25
cell_line
epithelial
cell_type
not collected
collection date
human
common name
Protocols: For two-dimensional co-culture, SCC25-tdTomato cells were seeded in a 1:1 ratio with mouse or naked mole-rat fibroblasts in DMEM/F12 medium (11320-032, Life Technologies) supplemented with 0.05% FBS (Life Technologies), 400 ng/ml hydrocortisone (Santa Cruz Biotechnology) and 1% Penicillin-Streptomycin (PEST) and cultured in a 37°C incubator with 5% CO2. After 48 hours SCC25-tdTomato and fibroblasts were separated using FACS sorting (E-cadherin-APC staining to separate SCC25 from fibroblasts (TdTom &E-cadherin-). Approximately 50,000-100,000 cells were sorted of each cell type from each condition. Total RNA was extracted by adding 800 µl TRIzol reagent (Life Technologies). Total RNA samples were DNased treated using Turbo DNase (Life Technologies). RNA-sequencing libraries prepared using Illumina stranded total RNA Prep, Ligation with RiboZero Plus (Illimuna) following manufacturer's instructions.
description
adult
developmental stage
squamous cell carcinoma
disease
wild type genotype
genotype
not collected
geographic location (country and/or sea)
SCC25 cells co-cultured with naked mole-rat fibroblasts
growth condition
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
tongue
organism part
Homo sapiens
scientific_name
scc25-nf_scc25_01
title