2025-12-31
ENA first public
ERC000037
ENA-CHECKLIST
CUNI
INSDC center name
public
INSDC status
ERS20917038
SRA accession
AA347_SAB_4_L
Submitter Id
21/18 °C day/night
air temperature regimen
2023
collection date
DNA isolated from A. arenosa mature leaves.
description
Slovakia
geographic location (country and/or sea)
48.26694
DD
geographic location (latitude)
19
DD
geographic location (longitude)
growth chamber
growth facility
Seeds were surface sterilized with 5% sodium hypochlorite and 0.01% (v/v) of Triton X-100 and planted on Petri dishes with Murashige and Skoog medium (1/2× MS: M0222 Duchefa, 0.8% agar, 10 mM MES, pH 5.8). Planted seeds were stratified for 7 days at 4 °C in the dark and then germinated for 2 weeks at 21/18 °C and 16/8 hours day/night cycle. Seedlings were transferred to soil and cultivated in the same conditions for another 5 weeks. After this, the plants were vernalized for at least two months at 4 °C and 8/16 hours day/night cycle. Vernalized plants were grown under long-day conditions (21/18 °C and 16/8 hours day/night cycle) to induce flowering. Young leaves were collected into 2ml Eppendorf tubes. All samples were kept at -80 °C for further use. DNA was extracted from leaves using DNeasy® Plant Mini Kit (Qiagen).
isolation and growth condition
16/8 hours day/night
light regimen
Arabidopsis arenosa
organism
PO:0025034
plant developmental stage
soil
plant growth medium
BTO:0000713
plant structure
diploid
ploidy
manual crossing
propagation
DNA was extracted from leaves using DNeasy® Plant Mini Kit (Qiagen).
sample collection method
healthy
sample health state
adult plant
sampled age
Arabidopsis arenosa
scientific_name
AA347_SAB
source material identifiers
Pannonian
subspecific genetic lineage name
phylogenetic lineage
subspecific genetic lineage rank
AA_12_L
title