2025-12-31
ENA first public
ERC000011
ENA-CHECKLIST
Laboratory of Traslational Research, Azienda USL-IRCCS di Reggio Emilia
INSDC center name
public
INSDC status
ERS20214524
SRA accession
E-MTAB-14152:H3K4me1_rep1
Submitter Id
ArrayExpress
broker name
MDA-T41
cell_line
Epithelial-like
cell_type
not collected
collection date
human
common name
Protocols: 20M cells were collected for Chromatin Immunoprecipitation. MDA-T41 cells were grown in RPMI-1640 supplemented with 1% non-essential amino acids, 10% FBS and 1% penicillin-streptomycin. All cell lines were grown at 37°C/ 5% CO2 and routinely tested for mycoplasma infection. ChIP samples were purified with PCR Purification Kit (Qiagen) and eluted in 50ul of Elution Buffer. Libraries were obtained starting from 3ng of ChIP and Input samples following ThruPLEX DNA-Seq Kit (Takara Bio Ink) protocol.
description
adult
developmental stage
papillary thyroid carcinoma
disease
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
thyroid gland
organism part
1
replicate
Homo sapiens
scientific_name
H3K4me1_rep1
title