2025-12-31
ENA first public
ERC000011
ENA-CHECKLIST
Institute of Metabolic Science - Metabolic Research Laboratories, University of Cambridge
INSDC center name
public
INSDC status
ERS27227470
SRA accession
E-MTAB-16104:IGTcontrol2
Submitter Id
14
week
age
ArrayExpress
broker name
not collected
collection date
house mouse
common name
Protocols: Mice were killed by cervical dislocation, pancreata were inflated with collagenase solution at 1 mg/ml, resected and placed in a water bath at 37°C for 10 min. After washing, islets were purified on a Histopaque-1119 / Histopaque-1083 gradient. Islets were cultured overnight at 37oC and 5% CO2 for respirometry, insulin secretion and enzyme studies, but were lysed ~3-4 hours after isolation for RNAseq analysis. Total RNA was isolated from islets using the ReliaPrep™ RNA Cell Miniprep System (Promega), according to the manufacturer’s instructions. RNA quality was validated using an Agilent Bioanalyzer 2100. All samples had RIN values between 7.4 to 10.  500 ng of total RNA was used for library construction using Illumina’s TruSeq Stranded mRNA prep kit following the manufacturer’s instructions. Indexed libraries were purified and validated using an Agilent Bioanalyzer 2100. Sequencing libraries were pooled at equal molar concentration.
description
adult
developmental stage
regular chow
diet
Kir6.2-V59Mfl/wt
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
islet of Langerhans
organism part
Mus musculus
scientific_name
female
sex
C57BL6/J
strain
IGTcontrol2
title