2025-12-31
ENA first public
ERC000011
ENA-CHECKLIST
ISABIAL
INSDC center name
public
INSDC status
ERS24442755
SRA accession
E-MTAB-15129:SC2_Sample2_CMO
Submitter Id
57.5 (8.8)
year
age
ArrayExpress
broker name
peripheral blood mononuclear cell
cell type
not collected
collection date
human
common name
Protocols: Human volunteers were recruited through the Service of Neurology of the Hospital General Universitario Dr. Balmis (HGUDrB), and consisted on 4 controls (without the HD mutation) and 4 HD symptomatic patients, with equal representation of both sexes in each condition (2 women and 2 men) and balanced age. Human blood samples were collected in EDTA-K2 Vaccutainer tubes (BD) for isolation of peripheral blood mononuclear cells (PBMC) after a density gradient centrifugation using Pancoll human, 1.077 g/mL (PAN-Biotech) following this procedure: 1,300xg 15min, followed by a wash with 0.1M PBS 1,000xg 5min. Cells were immediately cryopreserved in two aliquotes per donor using the Demonstrated Protocol CG00039 RevD “Fresh Frozen Human PBMC for scRNA-seq” (10xGenomics).  For the first assay (SC1), we created two pools (CTRL and HD) from samples; briefly, an aliquot of each cryopreserved sample was thawed and washed following the Demonstrated Protocol CG00039 RevD “Fresh Frozen Human PBMC for scRNA-seq” (10xGenomics), and subsequently sorted in a FACSAria III (Becton Dickinson) to remove cell debris and platelets and to collect equal number of cellular events (~4,500). For the second assay (SC2), we repeated the same procedure with the same samples with the exception of including a prior labelling procedure for each individual sample using Cell Multiplexing Oligo (CMO) tags linked to cell-associated barcodes. Next, CTRL and HD pools were processed with the Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1 (Dual Index) protocol to obtain single cell barcodes. We followed the Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1 (Dual Index) protocol
description
adult
developmental stage
Huntington disease and related disorders
disease
CAG expansion
genotype
not collected
geographic location (country and/or sea)
5-8
individual
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
blood
organism part
Homo sapiens
scientific_name
Women-Men
sex
SC2_Sample2_CMO
title