2025-12-31
ENA first public
ERC000011
ENA-CHECKLIST
College de France
INSDC center name
public
INSDC status
ERS28356051
SRA accession
E-MTAB-16400:AD_REP2_S_09
Submitter Id
ArrayExpress
broker name
O4 Oligodendrocyte
cell type
not collected
collection date
house mouse
common name
Protocols: Samples derive from MACS-isolated O4+ oligodendrocytes. RNA was isolated from oligodendrocytes using the RNA Micro Kit (Qiagen).  Libraries were prepared with the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (#E6420S, New England Biolabs) and NEBNext Multiplex Oligos for Illumina (Index Primers 1 12) (#E7335S, New England Biolabs), following the corresponding instruction manual (Version5.0_5/20). The protocol started with 10 ng total RNA when available (otherwise, entire sample). Full-length cDNA was generated by template switching, followed by cDNA amplification and cleanup; 1 μl amplified cDNA was evaluated on an Agilent High Sensitivity DNA chip (#5067-4626, Agilent Technologies).
description
Adult
developmental stage
Alzheimer's disease
disease
3xTg-AD
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
brain
organism part
Mus musculus
scientific_name
male
sex
C57BL/6
strain
AD_REP2_S_09
title