2025-12-31
ENA first public
ERC000011
ENA-CHECKLIST
nbdeyy
INSDC center name
public
INSDC status
ERS27094927
SRA accession
E-MTAB-15763:miRNA_BM-LLCExos
Submitter Id
ArrayExpress
broker name
BM-LLC
cell line
lung cancer cell
cell type
not collected
collection date
house mouse
common name
Protocols: Exosomes were collected and purified from the cell culture supernatant of NC-LLC and BM-LLC cells via differential ultracentrifugation. Lewis lung carcinoma (LLC) cells (NC-LLC and BM-LLC) were cultured in Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10% exosome-depleted fetal bovine serum (FBS) at 37°C in a 5% CO₂ atmosphere. Total RNA, including small RNAs, was extracted from the purified exosomes using the miRNeasy Serum/Plasma Kit (Qiagen) according to the manufacturer's instructions. Small RNA libraries were constructed using the NEBNext Multiplex Small RNA Library Prep Kit for Illumina. Briefly, small RNA molecules were ligated to 3' and 5' adapters, reverse transcribed, and amplified by PCR. The final cDNA libraries were size-selected to enrich for fragments containing microRNAs.
description
normal
disease
not collected
geographic location (country and/or sea)
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
Mus musculus
scientific_name
miRNA_BM-LLCExos
title