2025-12-31
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA114503801
External Id
Viral Research and Experimental Medicine Centre, SingHealth Duke-NUS Academic Medical Centre, Singapore
INSDC center name
2023-10-17T10:53:55Z
INSDC last update
public
INSDC status
ERS16485450
SRA accession
E-MTAB-13441:DBL-17
Submitter Id
21
year
age
ArrayExpress
broker name
mononuclear cell
cell_type
Dengue seropositive
clinical history
not collected
collection date
human
common name
Protocols: Blood was collected in EDTA tubes from subjects, and peripheral blood mononuclear cells (PBMCs) were isolated by density gradient centrifugation with SepMate-50 tubes (Stemcell Technologies) according to manufacturer’s instructions. Frozen PBMCs were thawed and cells were counted using Nexcellom Cellometer Spectrum, and cell viability assessed using Live/DEAD Viability/Cytotoxicity Kit for mammalian cells (Thermo Scientific). Cells are then partitioned into nanoliter-scale gel beads-in-emulsion (GEMs). Gel beads are dissolved, primers are released and any co-partitioned cell is lysed. Incubation of GEMs generates barcoded, full length cDNA from poly-adenylated mRNA. Single cell library preparation was performed using 10x Genomics Chromium Next GEM Single Cell 3’ Reagent Kits v3.1 (Dual Index) by Molecular Genomics Pte Ltd. Profiles of cDNA samples and the final libraries were verified using Agilent High Sensitivity D5000 screentapes on an Agilent Tapestation 4200. At least 5000 cells were targeted per sample with at least 20,000 reads sequenced per cell.
description
adult
developmental stage
normal
disease
not collected
geographic location (country and/or sea)
DBL-17
individual
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
blood
organism part
Homo sapiens
scientific_name
female
sex
DBL-17
title