characteristics.text: 2026-01-01
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: Department of Medicine 3 – Rheumatology and Immunology, Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg and Uniklinikum Erlangen
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS24603093
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-15133:RaSCS2
characteristics.characteristic: Submitter Id
characteristics.text: 71
characteristics.unit: year
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Biopsies were taken either at autopsy or during surgery. The instrument was inserted to bone contact and then gently withdrawn to obtain the biopsy (5 mm) 5 mm above the bone. Biopsies were immediately transferred into saline. Entheseal tissues were then minced and digested in RPMI 1640 containing 2 mg ∙ ml−1 collagenase D (Roche), 0.2 mg ∙ ml−1 Dispase II (Sigma-Aldrich) and 0.2 mg ∙ ml−1 DNase I (Roche). Samples were incubated three times at 37 °C for 20 min with constant shaking (2,000 rpm) on a thermal shaker (Eppendorf). After 20 min, the supernatant was collected, filtered (70 µm) and fresh digestion medium was added to the samples. The digestion was repeated for a total of 3 times. RBC lysis was performed using RBC lysis buffer (BioLegend) according to the manufacturer’s recommendations. Cells were washed, counted and concentrated to 1,000 cells / µl.    15,000 and 1,000 cells from healthy or inflamed enthesis were loaded into a single well of a Chromium chip G, respectively (10x Genomics) immediately after tissue dissociation and further processed according to standard Chromium Next GEM Single Cell 3' Gene Expression protocol (10x Genomics protocol CG000316 Rev C).   3’ gene expression library was generated using Chromium Next GEM Single Cell 3' Kit 3.1 Library Construction Kit .
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: normal
characteristics.characteristic: disease
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: enthesis
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: RaSCS2
characteristics.characteristic: title