characteristics.text: SAMN38726839
characteristics.characteristic: External Id
characteristics.text: National Research Council (IFC-CNR)
characteristics.characteristic: INSDC center name
characteristics.text: 2025-12-31T00:56:38.133Z
characteristics.characteristic: INSDC first public
characteristics.text: 2025-12-31T00:56:38.133Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS19808508
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Human
characteristics.characteristic: NCBI submission model
characteristics.text: Human.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS19808508
characteristics.characteristic: SRA accession
characteristics.text: not applicable
characteristics.characteristic: age
characteristics.text: not applicable
characteristics.characteristic: biomaterial_provider
characteristics.text: HEK293T
characteristics.characteristic: cell_line
characteristics.text: not applicable
characteristics.characteristic: collection_date
characteristics.text: independent clone from HEK293T stably transfected with rtTA, transduced with inducible constructs encoding for rat APOBEC1 and human RBM47, induced with doxycycline. In order to generate inducible APOBEC1 and RBM47 expressing cell lines, we used Tet-inducible APOBEC1 and RBM47 lentiviral expression vectors in HEK293-T cell lines expressing rTTA3. The APOBEC1 expression construct bore a EGFP coding sequence downstream the APOBEC1 one, connected through an IRES element. Cells were transduced sequentially with the lentiviral constructs and sorted after induction of the EGFP to obtain single clones. APOBEC1 expression was induced by doxycycline (2 ug/ml doxycycline for 48 hours [Sigma-Aldrich #D9891]). Two days post-induction cells were collected and the RNA was extracted.
characteristics.characteristic: description
characteristics.text: not applicable
characteristics.characteristic: geo_loc_name
characteristics.text: clone 87 APOBEC1+RBM47 +doxycycline
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: not applicable
characteristics.characteristic: sex
characteristics.text: not applicable
characteristics.characteristic: tissue
characteristics.text: Human sample from Homo sapiens
characteristics.characteristic: title