characteristics.text: 2026-01-01
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: kyung Hee University
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS25143684
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-15310:Control3
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: Japanese rice
characteristics.characteristic: common name
characteristics.text: Dongjin
characteristics.characteristic: cultivar
characteristics.text: Protocols: The samples were collected from paddy field at anthesis stage. We froze the anthers immediately using liquid nitrogen. Plants were transplanted to paddy field after two weeks in incubator at BOD incubator at end of May. The plants were then grown for 3 months in the field. Mature anthers were frozen in LN2 and finely ground using mortar. Grounded samples were homogenized using TRI reagents (RNAiso, Takara) and BCP (1-bromo-3-chloropropane). DNA, RNA, and protein layers were separated by centrifugation. Then, total RNA was extracted using the RNeasy Mini Kit (QIAGEN). The sequencing library is prepared by random fragmentation of the DNA or cDNA sample, followed by 5' and 3' adapter ligation. Alternatively, \\"tagmentation\\" combines the fragmentation and ligation reactions into a single step that greatly increases the efficiency of the library preparation process. Adapter-ligated fragments are then PCR amplified and gel purified.
characteristics.characteristic: description
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Oryza sativa Japonica Group
characteristics.characteristic: organism
characteristics.text: Oryza sativa Japonica Group
characteristics.characteristic: organism
characteristics.text: anther
characteristics.characteristic: organism part
characteristics.text: Oryza sativa Japonica Group
characteristics.characteristic: scientific_name
characteristics.text: Control3
characteristics.characteristic: title