characteristics.text: 2026-01-01
characteristics.characteristic: ENA first public
characteristics.text: ERC000022:1.6
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: University of Neuchatel
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS27198357
characteristics.characteristic: SRA accession
characteristics.text: BC-5-B-T2
characteristics.characteristic: Submitter Id
characteristics.text: temperate deciduous broadleaf forest [ENVO:01000385]
characteristics.characteristic: broad-scale environmental context
characteristics.text: 2024-08-28
characteristics.characteristic: collection date
characteristics.text: 0-0.1
characteristics.unit: m
characteristics.characteristic: depth
characteristics.text: Bois Chamblard bulk soil sampled with auger at four different spots on a 20x20 cm area and 12 cm depth, from a block of soil of 20x20x15cm (L x l x d) maintained at same location for 9 months, sieved at 2mm
characteristics.characteristic: description
characteristics.text: 392
characteristics.unit: m
characteristics.characteristic: elevation
characteristics.text: soil [ENVO:00001998]|plant litter [ENVO:01000628]
characteristics.characteristic: environmental medium
characteristics.text: BC T2 B
characteristics.characteristic: experimental factor
characteristics.text: Switzerland
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: 46.46601
characteristics.unit: DD
characteristics.characteristic: geographic location (latitude)
characteristics.text: 6.41102
characteristics.unit: DD
characteristics.characteristic: geographic location (longitude)
characteristics.text: Buchillon
characteristics.characteristic: geographic location (region and locality)
characteristics.text: soil surface layer [ENVO:04000010]|litter layer [ENVO:01000338]
characteristics.characteristic: local environmental context
characteristics.text: 1035
characteristics.unit: mm
characteristics.characteristic: mean annual precipitation
characteristics.text: 10.4
characteristics.unit: ºC
characteristics.characteristic: mean annual temperature
characteristics.text: bc1013:CTCTCGCATACGCGAG; bc1057:GACGAGCGTCTGAGAG
characteristics.characteristic: multiplex identifiers
characteristics.text: DNAeasy PowerSoil Pro Kit, QIAGEN
characteristics.characteristic: nucleic acid extraction
characteristics.text: uncultured eukaryote
characteristics.characteristic: organism
characteristics.text: 5.34
characteristics.characteristic: pH
characteristics.text: five grams of dry soil mixed in 12.5 ml of distilled water
characteristics.characteristic: pH method
characteristics.text: 616sfm1:TTAAAAMGCTCGTAGTYG; 616sfm2:TTAAAWCARTTATWRTYT; 28s3rm:ATTAAACWGTYRGATTCCC
characteristics.characteristic: pcr primers
characteristics.text: Fishing Active Soil Protists
characteristics.characteristic: project name
characteristics.text: 0.3
characteristics.unit: g
characteristics.characteristic: sample volume or weight for DNA extraction
characteristics.text: uncultured eukaryote
characteristics.characteristic: scientific_name
characteristics.text: 2 mm
characteristics.characteristic: sieving
characteristics.text: 60
characteristics.characteristic: slope aspect
characteristics.text: 1
characteristics.unit: %
characteristics.characteristic: slope gradient
characteristics.text: 18S; ITS; 28S
characteristics.characteristic: target gene
characteristics.text: Bois Chamblard T2 B
characteristics.characteristic: title
characteristics.text: 5.52405166625977
characteristics.unit: g/kg
characteristics.characteristic: total organic carbon
characteristics.text: Thermo ScientificTM FLASH 2000 CHNS elemental analyzer
characteristics.characteristic: total organic carbon method