characteristics.text: 2026-01-01
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: Japan Tobacco Inc.
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS22987037
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-14782:Sample 1
characteristics.characteristic: Submitter Id
characteristics.text: 20
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: epithelial cell
characteristics.characteristic: cell_type
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: HPAEpiCs on ALI day 28 were washed with PBS, incubated in 0.25% trypsin-EDTA, detached from the inserts, and separated by pipetting. Trypsinization was stopped by adding Dulbecco’s Modified Eagle’s Medium supplemented with 10% fetal bovine serum. The cell suspension was then centrifuged, washed, and passed through a 40 µm Flowmi cell strainer (Bel-Art Products, Wayne, NJ, USA). The obtained cells were fixed with 4% formaldehyde in PBS, and subsequently treated with the Quenching Buffer and Enhancer contained in a Chromium Next GEM Single Cell Fixed RNA Sample Preparation Kit (10x Genomics). Cell barcoding, amplification, and library construction were performed using a Chromium Fixed RNA Profiling Reagent Kit (10x Genomics) in accordance with the manufacturer's protocol. Isolated single cells were loaded onto a 10x Chromium X (10x Genomics), barcoded, and pre-amplified to construct the sequencing library.
characteristics.characteristic: description
characteristics.text: embryo stage
characteristics.characteristic: developmental stage
characteristics.text: normal
characteristics.characteristic: disease
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: Donor 1
characteristics.characteristic: individual
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: alveolus of lung
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.characteristic: sex
characteristics.text: Sample 1
characteristics.characteristic: title