characteristics.text: 2026-01-01
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: Centre Interdisciplinaire de Recherche en Biologie (CIRB) - Collège de France
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS27131880
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-15856:Scramble_1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: LUHMES
characteristics.characteristic: cell line
characteristics.text: dopaminergic neuron
characteristics.characteristic: cell type
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: LUHMES cells were differentiated for 7 days. Cells were transfected with siRNA at day 3 and retrieved at day 7 of differentiation (siRNA Control or siRNA ORF1). Lund human mesencephalic cells (LUHMES) cells were differentiated for 7 days, using  Advanced DMEM/F12 added with 1% N-2 supplement and GlutaMax, supplemented with 1 µg/ml doxycycline (Sigma), 2 ng/ml recombinant human GDNF (Peprotech, # 450-10) and 1mM cAMP (Sigma, # D0627). Cells were transfected with siRNA at day 3 and retrieved at day 7 of differentiation (siRNA Control or siRNA ORF1) RNA was harvested using Direct-zol™ RNA Microprep (Zymo, # R2062). Libraries including rRNA removal were generated using the RNA Library Prep Kit for Illumina following manufacturer’s recommendations.
characteristics.characteristic: description
characteristics.text: 7 days differntiated LUHMES cells
characteristics.characteristic: developmental stage
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: biological replicat 1
characteristics.characteristic: individual
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: Scramble_1
characteristics.characteristic: title