characteristics.text: 2026-01-01
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: Gyeongsang National University Animal Genomics Laboratory
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS26813155
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-15574:seo8
characteristics.characteristic: Submitter Id
characteristics.text: 3
characteristics.unit: year
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: dog
characteristics.characteristic: common name
characteristics.text: Protocols: A total of 24 beagle dogs aged 3 years (n = 8), 5 years (n = 8), and 10 years (n = 8) were investigated for age-related molecular signatures in their methylomes. All dogs were kept at the NIAS under standardized housing conditions that were maintained through continuous monitoring, vaccination, and anthelmintic treatment. The experiment was ethically approved by the Institutional Animal Care and Use Committee of the National Institute of Animal Science (NIAS 2022-586) and Gyeongsang National University (GNU-240802-D0152-01). Whole blood samples were collected using a 21-gauge needle and 5-mL syringe from the jugular vein of each dog. RNA from whole blood was extracted using a PAXgene Blood RNA Kit (762174; QIAGEN, Germantown, MD, USA) following the manufacturer’s instructions.  Libraries were prepared for 150 bp paired-end sequencing using a TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, CA, USA).
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Canis lupus familiaris
characteristics.characteristic: organism
characteristics.text: Canis lupus familiaris
characteristics.characteristic: organism
characteristics.text: blood
characteristics.characteristic: organism part
characteristics.text: Canis lupus familiaris
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: seo8
characteristics.characteristic: title