characteristics.text: 2026-01-01
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: EMBL Heidelberg
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS27961130
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-16292:Sample 44
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: MNT1
characteristics.characteristic: cell line
characteristics.text: MNT1
characteristics.characteristic: cell type
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Approximately 8*10^6 cells were lysed in 600 µl RLT buffer (Qiagen, #74181) and RNA was extracted using the RNeasy 96-well plate kit (Qiagen, #74181) according to manufacturer’s instructions including the DNase step.  Approximately 8*10^6 cells were lysed in 600 µl RLT buffer (Qiagen, #74181) and RNA was extracted using the RNeasy 96-well plate kit (Qiagen, #74181) according to manufacturer’s instructions including the DNase step.  The initial RNA was QCed using Agilent Bioanalyzer with the RNA Nano Assay kit as per the manufacturer’s protocol. The RNA sample set was then standardized to 200 ng total RNA in 50 ul using the concentration values given by the Bioanalyzer. The libraries were prepared on a Beckman Coulter Automated Workstation Biomek i7 Hybrid (MC + Span-8). For library preparation an automated version of the NEBNext® Ultra™ II Directional RNA Library Prep Kit was used, following section 1 - Protocol for use with NEBNext Poly(A) mRNA Magnetic Isolation Module 60. An adaptor dilution of 1 to 30 was used, the samples were individually barcoded using unique dual indices during the PCR using 12 PCR cycles as per the manufacturer’s protocol. The individual libraries were quantified using the Qubit HS DNA assay as per the manufacturer’s protocol. For the measurement 1ul of sample in 199ul of Qubit working solution was used. The quality and molarity of the libraries was assessed using Agilent Bioanalyzer with the DNA HS Assay kit as per the manufacturer’s protocol[v]. The assessed molarity was used to equimolarly combine the individual libraries into one pool for sequencing.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: cancer
characteristics.characteristic: disease
characteristics.text: BRAFV600E
characteristics.characteristic: genotype
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: zone of skin
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: Sample 44
characteristics.characteristic: title