characteristics.text: 2026-01-01
characteristics.characteristic: ENA first public
characteristics.text: ERC000025
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: NIOZ
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS22541174
characteristics.characteristic: SRA accession
characteristics.text: NIOZ369.0179.0192
characteristics.characteristic: Submitter Id
characteristics.text: environment determined by a biofilm on a saline surface [ENVO_01001056]| sea water environment [ENVO_01000321]
characteristics.characteristic: broad-scale environmental context
characteristics.text: 2022-06-10T13:15:00+01
characteristics.characteristic: collection date
characteristics.text: Nanoplastic impacted plastisphere communities
characteristics.characteristic: description
characteristics.text: water|biofilm|sediment
characteristics.characteristic: environment package
characteristics.text: coastal sea water [ENVO_00002150]
characteristics.characteristic: environmental medium
characteristics.text: Nanoplastics_Incubation
characteristics.characteristic: experimental factor
characteristics.text: Netherlands
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: +53.002
characteristics.unit: DD
characteristics.characteristic: geographic location (latitude)
characteristics.text: +4.80
characteristics.unit: DD
characteristics.characteristic: geographic location (longitude)
characteristics.text: Netherlands:Wadden Sea
characteristics.characteristic: geographic location (region and locality)
characteristics.text: biodegradation of plastic [ENVO_06105015]
characteristics.characteristic: local environmental context
characteristics.text: Northeast Atlantic Shelves (NECS)
characteristics.characteristic: longhurst zone code
characteristics.text: TAGGTAACCGATTAATCGGATTCC
characteristics.characteristic: multiplex identifiers
characteristics.text: https://www.qiagen.com/fi/~/media/5ADF5C38ED8749E1BA4C4966A215A83B.ashx
characteristics.characteristic: nucleic acid extraction
characteristics.text: plastisphere metagenome
characteristics.characteristic: organism
characteristics.text: initial denaturation:98degC_30sec;denaturation:98degC_10sec;annealing:50degC_15sec;elongation:72degC_30sec;final elongation:72degC_5 min;30 cycles
characteristics.characteristic: pcr conditions
characteristics.text: F: 515F (GTGYCAGCMGCCGCGGTAA) R: 926RBC (CCGYCAATTYMTTTRAGTTT)
characteristics.characteristic: pcr primers
characteristics.text: BDT5_NP1: Biodegradation Experiment with Nanoplastic Exposure
characteristics.characteristic: project name
characteristics.text: aerobe
characteristics.characteristic: relationship to oxygen
characteristics.text: 31
characteristics.unit: psu
characteristics.characteristic: salinity
characteristics.text: PHBH fragment
characteristics.characteristic: sample collection device
characteristics.text: samples stored in DNA lysis buffer
characteristics.characteristic: sample material processing
characteristics.text: plastisphere metagenome
characteristics.characteristic: scientific_name
characteristics.text: 16S rRNA| 18S
characteristics.characteristic: target gene
characteristics.text: V4-V6
characteristics.characteristic: target subfragment
characteristics.text: 20
characteristics.unit: ºC
characteristics.characteristic: temperature
characteristics.text: BDT5 NIOZ369.0179.0192
characteristics.characteristic: title