characteristics.text: 2026-01-01
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: I2BC_CEA/CNRS/Université Paris-Saclay
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS27185657
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-16030:M168_IR_2
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: M168
characteristics.characteristic: cell line
characteristics.text: fibroblast
characteristics.characteristic: cell type
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Cell monolayers were trypsinized, neutralized with medium, washed with PBS, and centrifuged. Washed cell pellets were resuspended in Macherey-Nagel Nucleospin RNA Plus Lysis Buffer. Not applicable- no dissections. DMEM + glutamine + pyruvate + pen/strep in 5% O2 + 5% CO2 incubator.  Proliferating cells were treated with 10 pg/ml Interleukin-alpha (Sigma I2778)for 24 hours. M168 cells were induced into senescence by irradiating with 40 Gy of 120 KV X-rays produced by a Faxitron 43855D generator. Cells were incubated for 10 days post irradiation before harvesting for RNA isolation. For IL1-alpha treatment of irradiated cells, the cells were irradiated with 40 Gy of 120 KV X-rays as above and cells were incubated for 9 days and then treated for 24 hours with 10 pg/ml of IL1-alpha (Sigma I2778). Data were normalized with EdgeR and Limma voom  RNA was extracted with the Macherey-Nagel Nucleospin RNA Plus. NEBNext Ultra II Directional RNA Library Kit with PolyA RNA selection
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: Female
characteristics.characteristic: genotype
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Breast skin
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: 40Gy_120kV_X-ray+10days
characteristics.characteristic: stimulus
characteristics.text: M168_IR_2
characteristics.characteristic: title