characteristics.text: 2026-01-01
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: Malaria Cell Biology, Molecular Biology and Immunology, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany Research Centre for Infectious Diseases, School of Biological Sciences, The University of Adelaide, Adelaide, Australia
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS25350382
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-15427:WT 1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: malaria parasite P. falciparum
characteristics.characteristic: common name
characteristics.text: Protocols: Cultures were tightly synchronised and harvested at late schizont. Cultures were pelleted, resuspended in TriZol (Invitrogen) and incubated for 5 minutes at 37°C before either immediate RNA extraction or freezing at -80°C. Chloroform (Sigma Aldrich) was added, mixed and then spun at 12,000g for 30 minutes at 4°C. Supernatant was collected and mixed with an equal volume of 70% Ethanol and processed using the RNeasy Kit (Qiagen) to extract purified RNA. RNA was DNAse l (Qiagen) treated for 30 mins at room temperature and cleaned up on RNeasy columns according to the manufacturers protocol. Absence of gDNA was checked by qPCR of gDNA and DNAse I treatment repeated if necessary. Library construction and sequencing was performed by Victorian Clinical Genetics Services (VCGS) Australia. Library was prepared using llumina stranded total RNA kit with Ribo -Zero Plus. QC was performed after libary prepration and prior to sequencing.
characteristics.characteristic: description
characteristics.text: Late Schizont
characteristics.characteristic: developmental stage
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Plasmodium falciparum
characteristics.characteristic: organism
characteristics.text: Plasmodium falciparum
characteristics.characteristic: organism
characteristics.text: whole organism
characteristics.characteristic: organism part
characteristics.text: Plasmodium falciparum
characteristics.characteristic: scientific_name
characteristics.text: 3D7
characteristics.characteristic: strain
characteristics.text: WT 1
characteristics.characteristic: title