characteristics.text: 2026-01-01
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: Tongren Hospital, School of Medicine,  Shanghai Jiao Tong University
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS27803152
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-16268:WT_12
characteristics.characteristic: Submitter Id
characteristics.text: 8
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: immune cell
characteristics.characteristic: cell type
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Wild-type mice were purchased from Shanghai SLAC Laboratory Animal Co. (Shanghai, China).All mice were housed in a Specific Pathogen Clearance (SPF) grade animal facility with a 12-hour light/dark cycle. Mice had free access to standard autoclaved feed and sterile water. All animal experimental procedures were conducted in accordance with the Guidelines for the Household and Use of Laboratory Animals and were approved by the Animal Ethics Committee.Following the stipulated immunization protocol, mice were humanely euthanized. Spleens were aseptically and surgically resected for subsequent single-cell suspension preparation and analysis. Single-cell suspensions were prepared from mouse spleens. Cell lysis and poly(A) RNA capture were performed simultaneously within the 10x Genomics GEMs, bypassing conventional RNA extraction. According to the manufacturer's instructions, a single-cell RNA-seq library was constructed using the Chromium Next GEM Single-Cell 5' Kit (10x Genomics). Cell lysis and reverse transcription were performed within the GEM to generate barcoded cDNA. The GEM was then lysed, the cDNA was purified, and amplified by PCR to generate the final sequencing library.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: wild type
characteristics.characteristic: genotype
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: pooled
characteristics.characteristic: individual
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: spleen
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: mixed
characteristics.characteristic: sex
characteristics.text: C57BL/6
characteristics.characteristic: strain
characteristics.text: WT_12
characteristics.characteristic: title