characteristics.text: 2026-01-01
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: University of Milan, Department of Medical Biotechnology and Translational Medicine
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS24937544
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-15221:5
characteristics.characteristic: Submitter Id
characteristics.text: 90
characteristics.unit: day
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Defeated and control animals underwent a final defeat session and were euthanized by decapitation 7 hours after sensory interaction to preserve brain tissue integrity. Hippocampi were dissected and snap-frozen in liquid nitrogen Total RNA was extracted from hippocampal extract with TRIzol Reagent (Invitrogen) following manufacturer protocol and resuspended in DEPC water. RNA concentration and quality was assessed by 4200 Tape Station (Agilent).  Universal Plus mRNA-Seq kit (Tecan Genomics, Redwood City, CA) has been used for library preparation following the manufacturer’s instructions (library type: fr-secondstrand). RNA samples were quantified and quality tested by Agilent 2100 Bioanalyzer RNA assay (Agilent technologies, Santa Clara, CA) or Caliper (PerkinElmer, Waltham, MA). Final libraries were checked with both Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA) and Agilent Bioanalyzer DNA assay or Caliper (PerkinElmer, Waltham, MA).
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: hippocampus
characteristics.characteristic: organism part
characteristics.text: A
characteristics.characteristic: rna sequencing batch
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: CSDS, resilient response
characteristics.characteristic: stimulus
characteristics.text: C57BL/6N
characteristics.characteristic: strain
characteristics.text: 5
characteristics.characteristic: title