characteristics.text: SAMN43368133
characteristics.characteristic: External Id
characteristics.text: L68550
characteristics.characteristic: ID
characteristics.text: German Center for Neurodegenerative Diseases, Dresden
characteristics.characteristic: INSDC center name
characteristics.text: 2025-12-31T01:05:01.240Z
characteristics.characteristic: INSDC first public
characteristics.text: 2025-12-31T01:05:01.240Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS22457089
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Model organism or animal
characteristics.characteristic: NCBI submission model
characteristics.text: Model.organism.animal.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: Abhinav Soni & Tomohisa Toda
characteristics.characteristic: Owner
characteristics.text: SRS22457089
characteristics.characteristic: SRA accession
characteristics.text: DIV13
characteristics.characteristic: age
characteristics.text: not applicable
characteristics.characteristic: breed
characteristics.text: Abhinav Soni
characteristics.characteristic: collected_by
characteristics.text: 2021-03-08
characteristics.characteristic: collection_date
characteristics.text: not applicable
characteristics.characteristic: cultivar
characteristics.text: RNA sample collected from basal state primary cortical neurons treated with AAV-shControl from DIV8 to DIV13. Sample was sequenced on Illumina NovaSeq 6000 in 100 bp paired-end mode.
characteristics.characteristic: description
characteristics.text: not applicable
characteristics.characteristic: dev_stage
characteristics.text: not applicable
characteristics.characteristic: ecotype
characteristics.text: Germany:Dresden
characteristics.characteristic: geo_loc_name
characteristics.text: Embryonic cortices (embryonic day 17.5-19.5) (C57/BL6 mice) from one litter were dissected and pooled together to be processed as a single biological replicate. Dissociated primary cortical neurons were plated in Poly-Dl-Ornithine Hydrobromide coated plates. Cells were maintained in incubators at 37°C with a CO2 concentration of 5%. 30% medium was replaced at DIV4 and DIV8 with Brain Phys Neuronal Culture Medium (STEMCELL Tec), supplemented with 2% SM1-NC (STEMCELL Tec).
characteristics.characteristic: growth_protocol
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: not collected
characteristics.characteristic: sex
characteristics.text: not applicable
characteristics.characteristic: strain
characteristics.text: Primary cortical neurons
characteristics.characteristic: tissue
characteristics.text: RNA_shNup153_2_Basal
characteristics.characteristic: title
characteristics.text: AAV treatment from DIV8 to DIV13 in basal state primary cortical neurons
characteristics.characteristic: treatment