characteristics.text: 2026-01-01
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: Hangzhou Hospital of Traditional Chinese Medicine, affiliated to Zhejiang University of Traditional Chinese Medicine
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS28360026
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-16416:T3
characteristics.characteristic: Submitter Id
characteristics.text: 74
characteristics.unit: year
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: Protocols: Tissue specimens were collected from surgically resected tumor samples of patients, snap-frozen at -80°C, and transferred in liquid nitrogen. This study was approved by the Ethics Committee. Total RNA was extracted from the samples using the [Insert Kit Name, e.g., TRIzol reagent / Qiagen RNeasy Mini Kit] according to the manufacturer's instructions. The concentration and purity of the RNA were determined by measuring the absorbance at 260 nm and 280 nm using a NanoDrop spectrophotometer (A260/A280 ratio). RNA integrity was assessed by agarose gel electrophoresis to verify the presence of intact 18S and 28S ribosomal RNA bands. The poly(A) mRNA isolation was performed using Oligo(dT) beads. The mRNA fragmentation was performed using divalent cations and high temperature. Priming was performed using Random Primers. First strand cDNA and the second-strand cDNA were synthesized. The purified double-stranded cDNA was then treated to repair both ends and add a dA-tailing in one reaction, followed by a T-A ligation to add adaptors to both ends. Size selection of Adaptor-ligated DNA was then performed using DNA Clean Beads. Each sample was then amplified by PCR using P5 and P7 primers and the PCR products were validated.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: colon carcinoma
characteristics.characteristic: disease
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: Patient_03
characteristics.characteristic: individual
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: human gut metagenome
characteristics.characteristic: organism
characteristics.text: human gut metagenome
characteristics.characteristic: organism
characteristics.text: colon
characteristics.characteristic: organism part
characteristics.text: human gut metagenome
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.characteristic: sex
characteristics.text: T3
characteristics.characteristic: title