characteristics.text: 2026-01-01
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: Institute for Rare Diseases Research, Instituto de Salud Carlos III (ISCIII).
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS20964913
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-14442:Dhx36 cKO
characteristics.characteristic: Submitter Id
characteristics.text: 7
characteristics.unit: day
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: heart cells
characteristics.characteristic: cell_type
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Whole hearts from 7-day-old PD7 wild-type (WT) (Dhx36f/f;Tnnt2 wt/wt) and mutant (Dhx36f/f;Tnnt2 cre/wt) mice were dissected, chopped into several pieces, snap-frozen and stored in liquid nitrogen. On the day of the experiment, wild-type and mutant hearts were pooled (two each), and nuclei were isolated using the Chromium Nuclei Isolation Kit with RNAse inhibitor (10X Genomics; #PN-1000494). Nuclei were captured in the Chromium Controller (10X Genomics) and processed with the Chromium Next GEM single cell Multiome ATAC+Gene Expression (GEX) Kit (10X Genomics; #PN-1000285) ATAC and GEX libraries from the same pool of pre-amplified Tn5 transposed DNA/cDNA
characteristics.characteristic: description
characteristics.text: PD7
characteristics.characteristic: developmental stage
characteristics.text: Dhx36 cardiomyocyte conditional KO
characteristics.characteristic: genotype
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: 2 hearts
characteristics.characteristic: individual
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: heart
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: mix
characteristics.characteristic: sex
characteristics.text: C57bl/6-CD1
characteristics.characteristic: strain
characteristics.text: Dhx36 cKO
characteristics.characteristic: title