characteristics.text: 2026-01-01
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: Department for Biomedical Research, University of Bern
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS25472922
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-15491:H02
characteristics.characteristic: Submitter Id
characteristics.text: 4 to 8
characteristics.unit: month
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: primary
characteristics.characteristic: cell line
characteristics.text: Aortic endothelial cell
characteristics.characteristic: cell type
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: pig
characteristics.characteristic: common name
characteristics.text: Protocols: To culture the the porcine endothelial cells using a microfluidic system, 100,000 cells in 100 μl of complete media were seeded in a μ-slide VI 0.4 (Ibidi, 80606) 16,21. Prior to seeding, the μ-slide was coated with 100 μl of 10 μg/ml fibronectin (Merck, FC010) for 30 min at 37 °C. Cells were incubated overnight before being connected to a peristaltic pump (Gilson, Minipuls 3) using sterile silicone tubings to introduce the flow. Flow media consisting of DMEM supplemented with 10% FBS, 1% glutamine, 1%BSA (Sigma, A7030), and 4% dextran (Sigma, 31390), was used as the flow medium. The laminar shear stress was adjusted to 10 dyn/cm2 and maintained for 72 h in a humidified incubator at 37 °C and with 5% CO2. The media was refreshed every day. For simulating xenogeneic activation, cells were perfused with 3 mL of flow media containing 10% pooled normal human serum (NHS) obtained from three healthy donors for 2 h. Additionally, cells were primed with 100 ng/ml recombinant human TNF-α (RnD Systems, 210-TA-020) for 4 h, prior to NHS perfusion, to simulate the inflammatory conditions in xenotransplantation settings.  Total cellular RNA from the all porcine endothelial samples was isolated using the NucleoSpin RNA kit (Macherey-Nagel, 740955) according to the manufacturer’s guidelines, followed by quantification of the total RNA with a Quantifluor RNA system (Promega, E3310). Bulk RNA barcoding and sequencing (BRB-seq) was performed by Alithea Genomics. A total of 200 ng of cellular RNA from four independent biological replicates was used to generate BRB-seq libraries,according to the company's pipeline.
characteristics.characteristic: description
characteristics.text: normal
characteristics.characteristic: disease
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Sus scrofa
characteristics.characteristic: organism
characteristics.text: Sus scrofa
characteristics.characteristic: organism
characteristics.text: aortic
characteristics.characteristic: organism part
characteristics.text: 4
characteristics.characteristic: replicate
characteristics.text: Sus scrofa
characteristics.characteristic: scientific_name
characteristics.text: H02
characteristics.characteristic: title