characteristics.text: 2026-01-01
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: Biotechnology and Biomedicine Center of the Academy of Sciences and Charles University in Vestec (BIOCEV); First Faculty of Medicine; Charles University, Czech Republic
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS24434733
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-15117:PE-08
characteristics.characteristic: Submitter Id
characteristics.text: 26
characteristics.unit: year
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Endometriotic tissues as well as samples of healthy endometrium were harvested laparoscopically at the time of the operation and immediately flash frozen in liquid nitrogen and stored in deep freeze until processing.  Frozen tissues were grinded with mortar and pestle and the total RNA was isolated with Trizol reagent following manufacturer's protocol. 1µg of DNase I-treated RNA isolated from tissues was used for poly-A mRNA isolation with the NEBNext Poly(A) mRNA Magnetic Isolation Module. Paired-end libraries were prepared using the NEBNext Ultra II Directional RNA Library Kit with NEBNext multiplex index oligos for Illumina (Dual Index Primers Set 1) (New England Biolabs).
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: Peritoneal endometriosis (PE)
characteristics.characteristic: disease
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: 8
characteristics.characteristic: individual
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: peritoneal cavity
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.characteristic: sex
characteristics.text: PE-08
characteristics.characteristic: title