SAMN44306765
External Id
Radboudumc
INSDC center name
2026-01-01T00:58:55.437Z
INSDC first public
2026-01-01T00:58:55.437Z
INSDC last update
SRS22922243
INSDC secondary accession
live
INSDC status
Model organism or animal
NCBI submission model
Model.organism.animal.1.0
NCBI submission package
SRS22922243
SRA accession
not applicable
age
not applicable
breed
U4.4 cell line
cell_line
wildtype
cell_subtype
ET
collected_by
2021-12-10
collection_date
not applicable
cultivar
ChIP-seq libraries were prepared with the Kapa Hyper Prep Kit for Illumina sequencing (Kapa Biosystems) according to the manufacturer's protocol with the following modifications. 3 ng DNA was used as input, with NEXTflex adapters (Bioo Scientific) and ten cycles of PCR amplification. Post-amplification cleanup and size selection was performed with dual AMPure XP beads selection (0.6× and 0.8×). Size-selected samples were analyzed for purity with a High Sensitivity DNA Chip on a Bioanalyzer 2100 system (Agilent), with an average size between 300 and 500 bp.
description
not applicable
dev_stage
not applicable
ecotype
Netherlands
geo_loc_name
wildtype U4.4 cells
isolate
ChIP-seq
library type
Aedes albopictus
http://purl.obolibrary.org/obo/NCBITaxon_7160
organism
wildtype
phenotype
biological replicate=1
replicate
not applicable
sex
U4.4 cells
strain
not applicable
tissue
H3K9me3_ChIP_wt_1
title
H3K9me3-ChIP
treatment