SAMN54325341
External Id
Washington University
INSDC center name
2025-12-30T00:00:00Z
INSDC first public
2026-01-02T16:02:20.390Z
INSDC last update
SRS27644311
INSDC secondary accession
live
INSDC status
Human
NCBI submission model
Human.1.0
NCBI submission package
SRS27644311
SRA accession
missing
age
missing
biomaterial_provider
2023-08-04
collection_date
To identify host cell factors required for LACV entry, we used a lentiviral library of 4,630 single guide RNAs targeting 1,146 human genes that encode proteins predicted to be present on the plasma membrane, and performed a CRISPR Cas9 screen in a haploid human cell line. For this screen, we took advantage of the rapid cytopathogenicity of vesicular stomatitis virus and generated a VSV-LACV chimera in which the native attachment and fusion glycoprotein of VSV was replaced with the M segment open reading frame of LACV. Gene-edited HAP1 cells were inoculated with VSV-LACV at a multiplicity of infection of 3, and genomic DNA from surviving cells was sequenced to identify enriched sgRNAs. The top hit from the screen was Jagged 2, a canonical Notch ligand and single-pass type I membrane protein. Two independent biological repeats were performed, and the raw data have been submitted.
description
USA
geo_loc_name
Homo sapiens
host
LACV
isolate
Human cells
isolation_source
Homo sapiens HAP1
lab_host
Homo sapiens
http://purl.obolibrary.org/obo/NCBITaxon_9606
organism
missing
sex
1960
strain
missing
tissue
HAP1 cells human surfesome CRIPSR screen
title