2026-01-07
ENA first public
ERC000011
ENA-CHECKLIST
IDIBAPS
INSDC center name
public
INSDC status
ERS27594676
SRA accession
E-MTAB-16259:M1ALC
Submitter Id
ArrayExpress
broker name
patient derived lymphoma spheroids
cell line
not collected
collection date
human
common name
Protocols: Peripheral blood was extracted and PBMC purified with Ficoll and cryopreserved in liquid nitrogen until the day of the experiment. Cells were grown with IL4, CD40L, HA-tag and BAFF as described in Araujo et al 2023 (PMID: 37031299) Silencing was performed using MALAT1 specific LNA-Gapmer (Qiagen) at 1micromolar vs Scrambled LNA-Gapmer (Qiagen) by gymnotic delivery along 5 days.  Total RNA was extracted using the RNeasy Plus Micro Kit (Qiagen), quantified with Qubit, and quality-checked using Tapestation 4200 (Agilent). Libraries were prepared from 10 ng of total RNA using the TAKARA_SMART-Seq® Total RNA Pico Input (ZapR® Mammalian) kit (following the manufacturer’s instructions).
description
adult
developmental stage
Mantle Cell Lymphoma
disease
not collected
geographic location (country and/or sea)
MCL#1
individual
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
Blood
organism part
Homo sapiens
scientific_name
female
sex
IL4/CD40L/HATAG/BAFF
stimulus
M1ALC
title