text: University of Maryland

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: From roots of greenhouse-grown black cohosh (Actaea racemosa), harvested on 09/09/2008. Total RNA was extracted with a Trizol- based method, mRNA selected with Qiagen's Oligotex mRNA Kit and used in library construction with Creator SMART cDNA Library Kit (Clontech). ESTs were cloned into pCR2.1, engineered to have SfiI sites in its MCS, and propagated in E. coli XL1-Blue cells. cDNA 5' ends were sequenced with M13R primer and vector sequences removed.

text: root

text: Actaea racemosa

text: SfiIA

text: SfiIB

text: hermaphrodite

text: Plant A26 (specimen voucher UMBI-SG-200902)

characteristic: strain

text: whole root tissues, without rhizome tissues

text: Black cohosh root library 1 (BCRT1)

text: Plasmid


status: PUBLIC

characteristic: External Id

text: University of Maryland

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: From rhizome of greenhouse-grown black cohosh (Actaea racemosa), harvested on 09/09/2008. Total RNA was extracted with a Trizol-based method, mRNA selected with Qiagen's Oligotex mRNA Kit, and used in library construction with Creator SMART cDNA Library Kit (Clontech). ESTs were cloned into pCR2.1 (Invitrogen), engineered to have SfiI sites in its MCS, and propagated in E. coli XL1-Blue cells. cDNA 5' ends were sequenced with M13R primer and vector sequences removed.

characteristic: description

text: rhizome

tag: attribute

characteristic: organ

text: Actaea racemosa

characteristic: organism

text: SfiIA

text: SfiIB

text: hermaphrodite

characteristic: sex

text: whole rhizome tissues, without root tissues

characteristic: tissue-type

text: Black cohosh rhizome library (BCRZ)

characteristic: title

text: Plasmid

characteristic: vector


text: University of Maryland

text: live

text: Generic

text: From rhizome of greenhouse-grown black cohosh (Actaea racemosa), harvested on 09/09/2008. Total RNA was extracted with a Trizol-based method, mRNA selected with Qiagen Oligotex mRNA Kit and used in library construction with T4 RNA Ligase- mediated RNA tagging as part of the ExactSTART Full-Length cDNA Library Cloning Kit (Epicentre). ESTs were cloned into pCDC1-K cloning vector and propagated in E. coli XL1-Blue cells. cDNA 5' ends were sequenced with M13R primer and vector sequences removed.

text: rhizome

text: Actaea racemosa

text: NotI

text: AscI

text: hermaphrodite

text: Black cohosh rhizome library 5 (BCRZ5)

text: Plasmid


text: USDA-ARS FRRL

text: live

text: Generic

text: The library was constructed and normalized as described by Bonaldo, M.F., Lennon, G. and Soares, M.B. (1996), Genome Research 6(9): 791-806. An identiying tag was added at the 5'during cDNA synthesis: ACGGCCGGG-insert (for shoot clones) or ACGGCCGAGGG-insert (for root clones)or ACGGCCAAGGG-insert (for crown clones) or ACGGCCTTGGG-insert (for seedling clones).

characteristic: lab-host

text: Dactylis glomerata

text: EcoRI (5' side of insert)

text: NotI (3' side of insert)

text: Shoots, roots, crowns and seedlings

text: BG04- primary and normalized libraries

text: Plasmid

text: pDONR-LIB


text: The Samuel Roberts Noble Foundation

text: live

text: Generic

text: A starter culture of each fungal strain was grown in 250 ml flasks containing 100 mls of 0.1X PD supplemented with antibiotics at 25oC with shaking for 5 days. The starter cultures were used to inoculate fresh 0.1X PD (3 replicates of each sample and time point). The fresh cultures of the An and VF fungi were grown at 25oC for 5 days with shaking. After the initial growth period, the control samples were kept at 25oC while the heat-stressed samples were moved to another incubator set at 38oC. Mycelial cultures were collected after 1, 3, 6, 9, 12 and 24 hours using filter papers and vacuum, immediately frozen in liquid nitrogen, lyophilized overnight and then kept at -80oC until further analyses. Two-hundred mg of each mycelia were grounded in liquid nitrogen and RNA was extracted using the RNeasy Mini Kit (Qiagene, Valencia, CA) followed by genomic DNA digestion with RNase-free DNase (Promega, Madison, WI). Equal amounts of total RNA from each biological replicate and time point were pooled prior to mRNA isolation using the PolyATtract mRNA isolation kit (Promega, Madison, WI). Four mRNA samples were generated: 1) An control grown at 25oC; 2) An heat grown at 38oC; 3) VF control grown at 25oC; and 4) VF heat grown at 38oC. Two ug of each mRNA was used for subtraction hybridization using the Clontech PCR-Select cDNA Subtraction kit (Clontech, Palo Alto, CA). Two subtractions were performed to enrich for viral induced transcripts at control conditions (VIC-ESTs) and viral induced transcripts at heat stress conditions (VIH-ESTs). Briefly, in the first subtraction, the mRNA sample isolated from the An control was used as the 'tester' cDNA and samples isolated from VF control was used as the 'driver' or the reference cDNA. This subtraction yielded enrichments of fungal transcripts induced by CThTV infection under normal growth conditions (VIC-ESTs). In the second subtraction, the mRNA sample isolated from the heat treated An was used as the 'tester' while the heat treated VF mRNA was used as the 'driver' cDNA. This subtraction yielded viral induced ESTs under heat stress (VIH-ESTs). The subtracted PCR products of each library were then cloned into pGEM-T Easy (Promega, Madison, WI) and transformed into DH5a competent E. coli cells. From each library, 2880 white bacterial colonies were grown in 384-deep-well microtiter plates followed by plasmid preparation and DNA sequencing with the M13 reverse primer using BigDye Terminator v3.1 (Applied Biosystem, Foster City, CA) followed by purification using Agencourt CleanSEQ Dye-Terminator Removal System (Beckman Coulter, Beverly, MA).

text: Mycelia

text: Curvularia protuberata

text: An: Carrying the Curvularia thermotolerance virus (CThTV) and VF: without the CThTV

text: VIC-ESTs and VIH-ESTs

text: pGMET-Easy


text: live

text: Generic

text: A laboratory strain of /S. littoralis/ was permanently reared on a semi-artificial diet at 23C, 60-70% relative humidity, under a 16:8 light:dark cycle and sexed as pupae. A normalization step was introduced in the library construction that consisted of a DNA target-RNA driver hybridization and substraction procedure (Invitrogen).

text: Spodoptera frugiperda

text: male antenna

text: Spodoptera frugiperda cDNA library, male antenna


text: National Chung-Hsing University

text: live

text: Generic

text: Anas platyrhynchos

text: female

text: magnum

text: Tsaiya ducks (Anas platyrhynchos) hatchability library


text: Texas A&M University

text: live

text: Generic

text: larval

characteristic: dev-stage

text: E.coli

text: Callosobruchus maculatus

text: midgut

text: cowpea bruchid larval midgut cDNA library

text: Plasmid

text: pBluescript SK(+)


text: live

text: Generic

text: The normalized cDNA library was made from pooled mRNAs extracted from hemocytes of 6th instar larvae injected with various foreign bodies (bacteria, virus, sephadex beads).

text: Spodoptera littoralis

text: immune-challenged hemocytes

text: Spodoptera littoralis cDNA library, immune-challenged hemocytes

text: pBS II SK(+)


text: live

text: Generic

text: The normalized cDNA library was made from pooled mRNAs extracted from different tissues and developmental stages.

text: Spodoptera littoralis

text: pooled tissues

text: Spodoptera littoralis cDNA library, pooled tissues

text: pBS II SK(+)


text: live

text: Generic

text: The cDNA library was made from pooled mRNAs extracted from midguts of 3rd instar larvae fed on artificial diet supplemented with Various natural products and xenobiotics.

text: Spodoptera littoralis

text: induced midguts

text: Spodoptera littoralis cDNA library, induced midguts

text: pSport I


text: International Centre for Genetic Engineering and Biotechnology and Directorate of Rice Research

characteristic: INSDC center name

text: live

text: Generic

characteristic: cultivar

text: Shoot

text: Oryza sativa Indica Group

text: Subtracted cDNA library from Indica rice variety TN1

text: Phage


text: Hokkaido University

characteristic: INSDC first public

characteristic: INSDC secondary accession

text: live

text: Generic

characteristic: SRA accession

text: Cells of Ochromonas danica were cultured in Ochromonas medium at 20 degree under continuous dim light condition.

text: Ochromonas danica

text: Ochromonas danica continuous dim light condition


characteristic: External Id

text: University of Cambridge

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Adineta ricciae

text: whole body


text: Institute of Chemistry and Dynamics of the Geosphere (ICG)

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Albena

text: mRNA was isolated from Sclerotinia-infected sunflower leaves and differentially expressed transcripts were selected by means of differential display analysis.

characteristic: description

text: Helianthus annuus

text: leaves

text: Helianthus annuus cv. Albena Sclerotinia-infected leaves


text: live

text: Generic

characteristic: isolate

text: Helianthus maximiliani

characteristic: organism

text: leaves

text: Helianthus maximiliani AC7 Sclerotinia-infected leaves


text: live

text: Generic

text: Sclerotinia sclerotiorum

text: Sclerotinia sclerotiorum isolate SS01

characteristic: title


text: Centro de BioInnovacion, Universidad de Antofagasta

text: live

text: Generic

text: abalone youthful exposed to copper exposure

text: youthful

text: Haliotis rufescens

text: soft tissue

characteristic: tissue-type

text: subtracted libraries from abalone youthful to copper exposure


text: National Institute for Biotechnology and Genetic Engineering

text: live

text: Generic

text: ESTs were obtained through differential display that appeared in wild type genotype when compared to lint-less mutant genotype.

text: Ovule

characteristic: organ

text: Gossypium hirsutum

text: hermaphrodite

characteristic: sex

text: Total ovule tissue

text: Differential display of Cotton Genotype, ovule (3 day post anthesis)

text: Plasmid

characteristic: vector


text: South China Normal University

characteristic: INSDC center name

text: live

text: Generic

text: Before low-temperature (vernalization) treatment, D. nobile plants were grown under a natural situation in the greenhouse of the School of Life Science, South China Normal University, China. Adult plants were selected for vernalization treatment at 15oC /10oC (day/night) for 20 and 30 days, respectively. A total of five to seven axillary buds closing to the shoot top were sampled from each seedling. Harvesting was done at the same point in the photoperiod to avoid the possible influence of circadian rhythms. The axillary buds that were collected from 20d- and 30d-vernalized plants were pooled for total RNA extraction and library construction. Full-length cDNA libraries were constructed using CreatorTM SMARTTM cDNA Library Construction Kit (Clontech) according to the manufacturer's instructions.

text: Vegitative development

characteristic: dev-stage

text: E. coli DH 5alpha

characteristic: lab-host

text: axillary bud

text: Dendrobium nobile

text: SfiI

text: SfiI

text: Dendrobium nobile (20+30)d-vernalized cDNA library

text: pDNR-LIB


text: South China Normal University

characteristic: INSDC first public

text: live

text: Generic

text: Before low-temperature (vernalization) treatment, D. nobile plants were grown under a natural situation in the greenhouse of the School of Life Science, South China Normal University, China. Adult plants were selected for vernalization treatment at 15oC /10oC (day/night) for 5 and 10 days, respectively. Five to seven axillary buds closing to the shoot top were sampled for each seedling. Harvesting was done at the same point in the photoperiod to avoid the possible influence of circadian rhythms. The axillary buds from various vernalized plants were collected respectively and the samples from 5 d- and 10 d-treatment were pooled for total RNA extraction and library construction. Full-length cDNA libraries were constructed using CreatorTM SMARTTM cDNA Library Construction Kit (Clontech) according to the manufacturer's instructions.

text: Vegitative development

text: E. coli DH 5alpha

text: axillary bud

text: Dendrobium nobile

text: SfiI

text: SfiI

text: Dendrobium nobile (5+10)d-vernalized cDNA library

text: pDNR-LIB


characteristic: External Id

text: South China Normal University

text: live

text: Generic

text: D. nobile plants were grown under a natural situation in the greenhouse of the School of Life Science, South China Normal University, China. Adult plants were selected for collectting 5-7 axillary buds close to the shoot top. Harvesting was done at the same point in the photoperiod to avoid the possible influence of circadian rhythms. The axillary buds from various plants were pooled for total RNA extraction. Full-length cDNA libraries were constructed using CreatorTM SMARTTM cDNA Library Construction Kit (Clontech) according to the manufacturer's instructions.

text: Vegitative development

text: E. coli DH 5alpha

text: axillary bud

text: Dendrobium nobile

text: SfiI

text: SfiI

text: Dendrobium nobile un-vernalized cDNA library

text: pDNR-LIB


text: South China Normal University

characteristic: INSDC last update

text: live

text: Generic

text: Before low-temperature (vernalization) treatment, D. nobile plants were grown under a natural situation in the greenhouse in School of Life Science, South China Normal University, China. Adult plants were selected for vernalization treatment at 15oC /10oC (day/night) for 40 days. A total of five to seven axillary buds closing to the shoot top were sampled from each seedling. Harvesting was done at the same point in the photoperiod to avoid the possible influence of circadian rhythms. The axillary buds from vernalized plants were collected and pooled for total RNA extraction and library construction. Full-length cDNA libraries were constructed using CreatorTM SMARTTM cDNA Library Construction Kit (Clontech) according to the manufacturer's instructions.

text: Vegitative development

text: E. coli DH 5alpha

text: axillary bud

text: Dendrobium nobile

text: SfiI

text: SfiI

text: Dendrobium nobile 40d-vernalized cDNA library

text: pDNR-LIB


text: live

characteristic: INSDC status

text: Generic

text: Coleochaete nitellarum

text: LB1261, University of Texas Culture Collection

characteristic: strain

text: Coleochaete nitellarum library


text: UAB Department of Vision Sciences

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Tupaia belangeri

text: Sclera

text: Scleral emmetropization genes


text: Inner Mongolia University of Science & Technology

text: live

text: Generic

text: cDNA-AFLP in Kalidium foliatum treated with 0, 100, 200, 300, 400 and 500 mmol/L NaCl for 48 hour.

characteristic: description

text: Kalidium foliatum

text: cDNA-AFLP in Kalidium foliatum treated NaCl


text: Indian Institute of Chemical Biology (A Unit of Council of Scientific and Industrial Research)

text: live

text: Generic

text: According to the instructions given in Clontech Manual on CreatorTM SmartTM cDNA Library Construction Kit (Cat. No. 634903; Clontech Lab Inc, USA)

text: Vegetative stage

text: Escherichia coli

text: Rhizome

text: Sinopodophyllum hexandrum

characteristic: organism

text: EcoRI

text: XhoI

text: cDNA library of Podophyllum hexandrum

text: pDNR-LIB


text: Hubrecht Institute

text: live

text: Generic

text: 24 hpf

text: Danio rerio

text: TL WT

text: whole embryo

text: Danio rerio whole embryo TL WT 24 hpf

characteristic: title


text: University of Trieste

text: live

text: Generic

text: juvenile

text: Dicentrarchus labrax

text: thymus

characteristic: tissue-type


text: Institute of Oceanology, Chinese Academy of Sciences

text: live

text: Generic

text: pediveliger, five days after fertilization

text: Meretrix meretrix

text: Meretrix meretrix pediveliger library


text: National Institute of Fruit Tree Science

text: live

text: Generic

text: Ballad

characteristic: cultivar

text: We isolated the genes whose expressions are higher (induced) or lower (depressed) in a spermidine synthase (SPDS)-overexpressing European pear, compared with a non-transformant (wild type). To this end, we used an annealing control primer (ACP)-based PCR (GeneFishing) system, a kind of differential display.

text: shoot with 5-6 cm in height

text: Pyrus communis

text: in vitro-cultured shoot

text: Pyrus communis in vitro-cultured shoot with 5-6 cm in height


text: Northwest A & F University

text: live

text: Generic

text: 2 days post inoculation

text: 4 weeks

text: Arabidopsis thaliana

text: leaf

text: Phytophthora parastica infected Arabidopsis leaf


text: live

text: Generic

text: The library consists of purified mRNAs using CloneMiner cDNA library construction kit (Invitrogen) which is designed to construct cDNAlibraries without traditional restriction enzyme cloning methods. This technology combines the performance of SuperscriptII reverse Transcriptase with the Gateway Technology. Single-standed mRNA is converted into double stranded cDNA containing attB sequences on each end. Through site-specific recombination, attB-flanked cDNA is cloned directly into attP-containing donor vector by homologue recombination. Library construction and sequencing were performed at Genoscope - Centre National de Sequencage (Evry, FRANCE).

text: Alvinella pompejana

text: pygidum

text: Alvinella pompejana pygidium library


text: Genoscope - Centre National de Sequencage

text: live

text: Generic

text: Alvinella pompejana

text: whole body

text: Alvinella pompejana whole body library


text: live

text: Generic

text: This library was constructed by oligo-capping method. Full length capped mRNAs were enriched by the action of the bacterial alcaline phosphatase to digest 5'-uncapped mRNAs. A 30-mer 5' oligo was linked using T4 RNA ligase after removing 5'cap using Tobacco acid pyrophosphatase. The 1st strand cDNA was primed with an oligo(dT)-SfI primer and double stranded using specific 5' and 3' primers and amplified by PCR. The PCR SfI-digested cDNA products were size selected to exclude fragments <1kb and then linked into pME18S-FL3 DraIII-digested vector. Library construction and sequencing were performed at Genoscope - Centre National de Sequencage (Evry, FRANCE).

text: Alvinella pompejana

text: DraIII

text: DraIII

text: gill

text: Alvinella pompejana gill library


text: live

text: Generic

text: Alvinella pompejana

text: DraIII

text: DraIII

text: ventral tissue

text: Alvinella pompejana ventral tissue library


text: Osaka City University, Department of Biology

text: live

text: Generic

text: mixture of vegetative cells and sporulating cells

characteristic: cell type

text: Schizosaccharomyces pombe

text: SPA (pGCAP10) cDNA library


text: Faculty of Biology, Plant Physiology

text: live

text: Generic

text: Seeds of Lepidium sativum (garden cress) were imbibed in RM medium at 18 degrees and continuous light for 8 and 18 hours, respectively. cDNA synthesised from RNA extracted from the endosperm cap after 8 hours was used as Tester, against cDNA from RNA, extracted from the endosperm cap after 18h imbibition as a Driver to enrich for early- germination expressed transcripts. The Suppression Subtraction Hybridisation library was constructed using the PCR Select cDNA Subtraction kit (Clontech, Mountain View, CA). The obtained cDNA fragments were cloned in pCR4-TOPO.

text: imbibed seed

text: E.coli TOP10

text: seed

characteristic: organ

text: Lepidium sativum

text: endosperm cap

text: early-germination upregulated pCR4-TOPO

characteristic: vector


text: Faculty of Biology, Plant Physiology

characteristic: INSDC center name

text: live

text: Generic

text: Seeds of Lepidium sativum (garden cress) were imbibed in RM medium at 18 degrees and continuous light for 8 and 18 hours, respectively. cDNA synthesised from RNA extracted from the endosperm cap after 8 hours was used as Tester, against cDNA from RNA, extracted from the endosperm cap after 18h imbibition as a Driver to enrich for early- germination expressed transcripts. The Suppression Subtraction Hybridisation library was constructed using the PCR Select cDNA Subtraction kit (Clontech, Mountain View, CA). The obtained cDNA fragments were cloned in pUC19.

text: imbibed seed

characteristic: dev-stage

text: E.coli Top10

text: seed

text: Lepidium sativum

text: endosperm cap

text: early-germination upregulated puc19


text: Middle East Technical University

characteristic: INSDC first public

text: live

text: Generic

text: Hordeum vulgare

text: leaf

text: Hordeum vulgare cv. Pallas-02 leaf


characteristic: External Id

text: University of Girona (Sciences Faculty)

text: live

text: Generic

text: Jaerla

text: The subtractive hybridization protocol from BD Biosciences Clontech (Palo Alto, CA) was used to generate this specific cDNA library. Tester cDNa was prepared from tuber periderm and driver from tuber parenchyma. This library contains differential cDNAs between both tissues that are positively expressed in periderm.

text: active and mature

text: potato tuber

text: Solanum tuberosum

text: Periderm

text: SSH library of potato tuber periderm

text: T/A plasmid vector

text: pCR4_TOPO (Invitrogen)


text: University of Girona (Sciences Faculty)

characteristic: INSDC last update

text: live

text: Generic

text: cork bark

text: Quercus suber

text: Periderm

text: Cork oak library of phellem

text: pCR4_TOPO (Invitrogen)


text: Alexindria University, Faculty of Science

text: live

characteristic: INSDC status

text: Generic

text: 10-day growth of culture

text: Azolla caroliniana

text: Aquatic fern (Pteridophyta) 10-day growth of culture


text: Hiroshima University

text: live

text: Generic

text: Ascidia gemmata

text: intestine

text: Ascidia gemmata intestine


text: University of Maryland

text: live

text: Generic

text: From young leaf of greenhouse-grown black cohosh (Actaea racemosa), harvested June 2007. Total RNA was extracted with a Trizol-based method (TIGR protocol for 'Total RNA extraction for plant tissue by Trizol'), mRNA selected with Qiagen's Oligotex mRNA Kit and used in library construction with the SuperScript Plasmid System with Gateway Technology for cDNA Synthesis and Cloning (Invitrogen). ESTs were cloned into pSPORT 1 and propagated in E. coli DH10B cells. cDNA 5' ends were sequenced with M13R primer and vector sequences removed.

text: young leaf

text: Actaea racemosa

text: NotI

text: SalI

text: hermaphrodite

characteristic: sex

text: Plant A52 (specimen voucher UMBI-SG-200903)

characteristic: strain

text: young leaf tissues

text: Black cohosh young leaf library (BCYL)

text: Plasmid

text: pSPORT 1


text: Sugarcane Breeding Institute

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: DD-RT-PCR derived fragments cloned in pTZ57R/T

text: Saccharum hybrid cultivar Co 93009

text: cell suspension

text: Cell suspension DD-RT-PCR transcript


text: Nanjing Normal University

text: live

text: Generic

text: Periplaneta americana

characteristic: organism

text: male

text: testis

text: Periplaneta americana male testis


text: Nanjing Normal University

text: live

text: Generic

text: Periplaneta americana

text: female

text: ovary

text: Periplaneta americana female ovary

characteristic: title


text: West China Research Institute for Health of Women and Children, West China Second University Hospital

text: live

text: Generic

text: Using assisted reproductive technology full term placenta as the tester and the normal as the driver, forward suppression subtractive hybridization (SSH) was performed, then cDNA fragments were cloned into the pGM-T vector to make a cDNA library.

characteristic: description

text: full term

text: Homo sapiens

text: EcoRI

text: HindIII

text: female

text: placenta

characteristic: tissue-type

text: human placenta differential expressed gene library forward

text: cosmid

text: pGM-T


text: live

text: Generic

text: Using assisted reproductive technology full term placenta as the driver and the normal as the tester, reverse suppression subtractive hybridization (SSH) was performed, then cDNA fragments were cloned into the pGM-T vector to make a cDNA library.

text: full term

text: Homo sapiens

text: EcoRI

text: HindIII

text: female

text: placenta

text: human placenta differential expressed gene library reverse

text: cosmid

text: pGM-T


text: Centro de Biotecnologia Genomica-Instituto Politecnico Nacional

text: live

text: Generic

text: SSH-INDY library obtained by Suppressive subtractive hybridization from yeast growth on minimal salts medium for 24 h without/with fungal cell walls

text: Meyerozyma guilliermondii

text: SSH-INDY


text: live

text: Generic

text: ESTs exclusively obtained by differential display from yeast growth on minimal salts medium for 24 h without carbon source

text: Meyerozyma guilliermondii

text: DD-NIY


text: live

text: Generic

text: ESTs exclusively obtained by differential display from yeast growth on minimal salts medium for 24 h and induced by extracellular metabolites produced by active P. digitatum enclosed in a membrane (YMF)

text: Meyerozyma guilliermondii

text: DD-YMF


text: live

text: Generic

text: ESTs exclusively obtained by differential display from yeast growth on minimal salts medium for 24 h and induced by direct contact with fungal cell walls

text: Meyerozyma guilliermondii

text: DD-INDY


text: Kunming University of Science and Technology

text: live

text: Generic

text: Tanbaguro

characteristic: cultivar

text: The suppression subtractive hybridization library for black soybean under aluminum (Al) stress. Alternate cultivar name 'Danbo'.

text: exposed to Al stress (50uM) for 0.5, 2, 4, 8, 12 and 24 hours respectively

characteristic: lab-host

text: root

text: Glycine max

text: root

text: SSH library for black soybean under aluminum (Al) stress

text: pMD18-T Vector


text: International Center for Tropical Agriculture, CIAT. http://www.ciat.cgiar.org

text: live

text: Generic

text: Total RNA was prepared from common bean roots subjected to low or high phosphorus conditions in a hydroponic chamber in the greenhouse of Pennsylvania State University, USA; poly(A) RNA was purified and reverse transcribed at the the International Center for Tropical Agriculture (CIAT), Cali, COLOMBIA, and cDNAs were cloned into UniZAP XR vector arms. The library was transformed into E. coli cells which were plated on petri plates with Carbenicillin anti-biotic (100 mg/L) and selected by blue-white screening for inserts. A Q-Bot was used to pick and array colonies into plates and filters.

text: E. coli XL-1-Blue MRF

text: Phaseolus vulgaris

text: EcoRI

text: XhoI

text: Root

text: Phaseolus vulgaris root EST library PV_DEd

text: Plasmid

text: UniZAP XR

characteristic: vector


characteristic: INSDC center name

text: live

text: Generic

text: E. coli XL-1-Blue MRF

text: Phaseolus vulgaris

text: EcoRI

text: XhoI

text: Root

text: Phaseolus vulgaris root EST library PV_DEe

text: Plasmid

text: UniZAP XR


text: Zhejiang Agriculture and Forestry University

text: live

text: Generic

text: Tinny Tender

text: The library was constructed using a Clontech PCR-Select cDNA Subtraction Kit (Clontech). In the subtractive hybridizations, the cDNA from the leaves under low temperature was used as the tester and cDNA from the leaves under higher temperature was used as the driver. The subtracted cDNA pool was randomly cloned into pMD18-T vector (TaKaRa).

text: x Doritaenopsis hybrid cultivar

text: leaf

text: Doritaenopsis leaf suppression subtractive cDNA library


text: Wuhan University

text: live

text: Generic

text: Leaves of submerged seedlings and the corresponding non-submerged controls, respectively, were harvested at 1, 3, 5, 8 d after treatment. The cDNA was reversely transcribed from 2 ug of mixed mRNA isolated at the various time points after treatment. The driver cDNA and the tester cDNA were produced from leaves of submerged seedlings and the corresponding controls, respectively. The SSH library was constructed with the Clontech PCR Select-cDNA Subtraction Kit (CLONTECH).

text: E.coli DH10B

text: leaf

characteristic: organ

text: Oryza sativa Indica Group

text: EcoRI

text: BamHI

text: FR13A Submergence VS control suppression subtractive hybridization reverse libraries

text: Plasmid

text: pCRII


text: Wuhan University

characteristic: INSDC first public

text: live

text: Generic

text: Leaves of submerged seedlings and the corresponding non-submerged controls, respectively, were harvested at 1, 3, 5, 8 d after treatment. The cDNA was reversely transcribed from 2 ug of mixed mRNA isolated at the various time points after treatment. The tester cDNA and driver cDNA were produced from leaves of submerged seedlings and the corresponding controls, respectively. The SSH library was constructed with the Clontech PCR Select-cDNA Subtraction Kit (CLONTECH).

text: E. coli DH10B

text: leaf

text: Oryza sativa Indica Group

text: EcoRI

text: BamHI

text: FR13A Submergence VS control suppression subtractive hybridization forward libraries

text: Plasmid

text: pCRII


characteristic: External Id

text: Wuhan University

text: live

text: Generic

text: Goda Heenati

text: Leaves of submerged seedlings and the corresponding non-submerged controls, respectively, were harvested at 1, 3, 5, 8 d after treatment. The cDNA was reversely transcribed from 2 ug of mixed mRNA isolated at the various time points after treatment. The tester cDNA and the driver cDNA were produced from leaves of submerged seedlings and the corresponding controls, respectively. The SSH library was constructed with the Clontech PCR Select-cDNA Subtraction Kit (CLONTECH).

text: E.coli DH10B

text: leaf

text: Oryza sativa Indica Group

text: EcoRI

text: BamHI

text: Goda Heenati Submergence VS control suppression subtractive hybridization forward libraries

text: Plasmid

text: pCRII


text: Wuhan University

characteristic: INSDC last update

text: live

text: Generic

text: Goda Heenati

text: E.coli DH10B

text: leaf

text: Oryza sativa Indica Group

text: EcoRI

text: BamHI

text: Goda Heenati Submergence VS control suppression subtractive hybridization reverse libraries

text: Plasmid

text: pCRII


text: Tokyo University of Marine Science and Technology

text: live

characteristic: INSDC status

text: Generic

text: Seriola quinqueradiata

text: liver

text: Seriola quinqueradiata liver


text: Chung-Ang University

text: live

text: Generic

text: Amynthas koreanus

text: EcoRI

text: XhoI

text: whole body

text: Amynthas koreanus whole body cDNA library

text: pCMV-Script XR


text: Genoscope - Centre National de Sequencage

text: live

text: Generic

text: A laboratory strain of /S. littoralis/ was permanently reared on a semi-artificial diet at 23C, 60-70% relative humidity, under a 16:8 light:dark cycle and sexed as pupae. A normalization step was introduced in the library construction that consisted of a DNA target-RNA driver hybridization and substraction procedure (Invitrogen).

text: Spodoptera littoralis

text: male antenna

text: Spodoptera littoralis cDNA library, male antenna


text: live

text: Generic

text: The normalized cDNA library was made from pooled mRNAs extracted from hemocytes of 6th instar larvae injected with various foreign bodies (bacteria, virus, sephadex beads).

text: Spodoptera frugiperda

text: immune-challenged hemocytes

text: Spodoptera frugiperda cDNA library, immune-challenged hemocytes

text: pBS II SK(+)


text: live

text: Generic

text: The normalized cDNA library was made from pooled mRNAs extracted from different tissues and developmental stages.

text: Spodoptera frugiperda

text: pooled tissues

text: Spodoptera frugiperda cDNA library, pooled tissues

text: pBS II SK(+)


text: live

text: Generic

text: The cDNA library was made from pooled mRNAs extracted from midguts of 3rd instar larvae fed on artificial diet supplemented with Various natural products and xenobiotics.

text: Spodoptera frugiperda

text: induced midguts

text: Spodoptera frugiperda cDNA library, induced midguts

text: pSport I


text: CIRAD Departement Systemes Biologiques

text: live

text: Generic

text: Transcript sequences from different tissues at various stages of development and treated by wounding or ethylene in Hevea brasiliensis clone PB260

text: Hevea brasiliensis

text: embryo, root, stem, bark, leaf

text: Hevea brasiliensis PB260 Transcript sequences


text: Huazhong Agriculture University

text: live

text: Generic

text: pycnidium

characteristic: cell type

text: SSH cDNA library (Clontech Laboratories, Inc.)

text: pycnidium development

characteristic: dev-stage

text: Paraphaeosphaeria minitans

text: mycelia

text: Coniothyrium minitans conidiation-associated gene Library

text: Plasmid


text: International Crops Research Institute for the Semi-Arid Tropics (ICRISAT)

text: live

text: Generic

characteristic: NCBI submission model

text: ICC 4958

text: cDNA library was constructed from root tissues of 25 days old chickpea seedling of ICC 4958 genotype imposed to dehydration for a period of 7 days in greenhouse conditions. The cDNA fragments were cloned into pGEM Easy vector and subsequently sequenced by Sanger sequencing approach using M13 primers.

text: E. coli (DH5a)

text: Cicer arietinum

text: EcoRI

text: root

text: ICC 4958 drought stressed root cDNA library

text: plasmid

text: pGEM Easy vector


text: live

text: Generic

characteristic: NCBI submission package

text: ICC 4958

text: The suppression subtractive hybridization process was carried out using ICC 4958 as the tester and Annigeri as the driver, the PCR products of the subtraction were cloned into the pCR4_TOPO cloning vector. The cDNA inserts were amplified with M13 forward and reverse primers and subsequently sequenced with T7 primer from the 3' end.

text: E. coli (DH10B)

text: Cicer arietinum

text: EcoRI

text: EcoRI

text: root

text: Cicer arietinum Suppressive substractive hybridization library


text: University of Dhaka

text: live

text: Generic

text: BJRI Tossa Pat-4 (O-72)

text: The sequence was obtained by mRNA differentially display.

text: 5-6 days old seedlings

text: Corchorus olitorius

characteristic: organism

text: Seedling

text: Corchorus olitorius Differentially expressed transcripts upon fungal (Macrophomina phaseolina) infection


text: University of Dhaka

text: live

text: Generic

text: 5-6 days old seedlings

text: Corchorus trilocularis

text: Seedling

text: Corchorus trilocularis Differentially expressed transcripts upon fungal (Macrophomina phaseolina) infection

characteristic: title


text: IFREMER

text: live

text: Generic

text: Zostera noltei

text: inner leaf

text: Zostera noltii 30 minutes into the heat shock library


text: IFREMER

text: live

text: Generic

text: Zostera noltei

text: inner leaf

text: Zostera noltii 2 hours into the heat shock library


text: IFREMER

text: live

text: Generic

text: Zostera noltei

text: inner leaf

text: Zostera noltii 4 hours into the heat shock library


text: IFREMER

text: live

text: Generic

text: Zostera noltei

text: inner leaf

text: Zostera noltii 24 hours into the heat shock library


text: IFREMER

text: live

text: Generic

text: Zostera noltei

text: inner leaf

text: Zostera noltii no heat shock library (control)


text: Genetic Engineering and Biotechnology Institute (GEBI)

text: live

text: Generic

text: 28 days-old seedling stage

text: Aegilops tauschii

text: leaf

text: Aegilops tauschii 150 mM NaCI treated leaf tissue during 8 hours


text: live

text: Generic

text: 28 days-old seedling stage

text: Aegilops tauschii

text: leaf

text: Aegilops tauschii 150 mM NaCI treated leaf tissue during 27 hours


text: College of Life Science, Wuhan University

text: live

text: Generic

text: male gamete

text: Sperm cells were manually collected from pollen tubes. RNA was isolated and cDNA library was constructed by using the Clontech SMART cDNA library construction kit. The cDNAs were directionally ligated into pTripEX2.

characteristic: description

text: Before fertilization

text: Nicotiana tabacum

text: Sfi IA

text: Sfi IB

text: Tobacco sperm cell cDNA library

text: Phage


text: Bose Institute

text: live

text: Generic

text: A Suppression subtractive hybridization (SSH) library was created using the Clontech PCR-Select cDNA Subtraction Kit using the mRNA from the MYMIV infected VM1 leaves as tester and mock inoculated VM1 leaves as driver at early stages of infection. The secondary PCR product was non-directionally cloned in pGEM-T easy vector (Promega) and sequenced.

text: Vigna mungo

text: Leaf

characteristic: tissue-type

text: SSH library of MYMIV infected Vigna mungo (VM1)


text: Madras Veterinary College, TANUVAS

text: live

text: Generic

text: Messenger RNA was isolated over a MACS mRNA isolation column (Miltenyi Biotec) then reverse transcribed and amplified into cDNA by long distance polymerase chain reaction (PCR) in a SMART cDNA synthesis kit (Clontech, USA). Briefly, double stranded cDNA was digested with Sfi I restriction enzyme, size fractionated using CHROMA SPIN - 400 and ligated into the TriplEx2 vector. A phage packaging reaction was performed using Gigapack II Gold (Stratagene) and the packaged phages transduced into E.coli BM25.8 and converted from TriplEx2 to pTriplEx2.

text: Sporozoites

text: chicken

text: Eimeria brunetti

text: Sfi I

text: Houghton

characteristic: strain

text: Sporozoites of Eimeria brunetti

text: Phagemid


text: National Institute for Plant Genome Research

text: live

text: Generic

text: Pennisetum sp. [a BC1 (2n=23, GGO) derivative of an aposporous inter-specific cross between P. glaucum x P. orientale ; obtained as [P. glaucum (2n=14, GG) x { P. glaucum (2n=14, GG) x P. orientale (2n=18, OO)}]

text: Pre-anthesis

text: Cenchrus americanus x Cenchrus orientalis

text: Subtractive cDNA library from aposporous Pennisetum sp.


text: Korea Research Institute of Bioscience and Biotechnology

text: live

text: Generic

text: adult

text: Macaca mulatta

text: female

characteristic: sex

text: placenta

text: full-length enriched mouse cDNA library


text: University of Oslo

text: live

text: Generic

text: Lepidodinium chlorophorum

text: Lepidodinium chlorophorum RCC1488 library


text: Faculty of Fisheries Technology and Aquatic Resources, Maejo University

text: live

text: Generic

text: blood cell

text: Pangasianodon gigas

text: male

text: blood

text: Pangasianodon gigas post-OV Library


text: live

text: Generic

text: blood cell

text: Pangasianodon gigas

text: male

text: blood

text: Pangasianodon gigas OV Library


text: Advanced Computational Sciences Department, RIKEN Advanced Science Institute (ASl)

text: live

text: Generic

text: Homo sapiens

text: Testis

text: Homo sapiens Testis (Srivastava)


text: live

text: Generic

text: Homo sapiens

text: Skeletal muscle

text: Homo sapiens Skeletal muscle (Srivastava)


text: live

text: Generic

text: Homo sapiens

text: Prostate

text: Homo sapiens Prostate (Srivastava)


text: live

text: Generic

text: Homo sapiens

text: Liver

text: Homo sapiens Liver (Srivastava)


text: live

text: Generic

text: Homo sapiens

text: Uterus

text: Homo sapiens Uterus (Srivastava)


text: live

text: Generic

text: Homo sapiens

text: Fetal kidney

text: Homo sapiens Fetal kidney (Srivastava)


text: live

text: Generic

text: Homo sapiens

text: Brain

text: Homo sapiens Brain (Srivastava)


text: live

text: Generic

text: Homo sapiens

text: Fetal brain

text: Homo sapiens Fetal brain (Srivastava)


text: live

text: Generic

text: Homo sapiens

text: Lung

text: Homo sapiens Lung (Srivastava)


text: live

text: Generic

text: Homo sapiens

text: Heart

text: Homo sapiens Heart (Srivastava)


text: live

text: Generic

text: Homo sapiens

text: Stomach

text: Homo sapiens Stomach (Srivastava)


text: live

text: Generic

text: Homo sapiens

text: Fetal liver

text: Homo sapiens Fetal liver (Srivastava)


text: live

text: Generic

text: Homo sapiens

text: Spleen

text: Homo sapiens Spleen (Srivastava)


text: live

text: Generic

text: Homo sapiens

text: Kidney

text: Homo sapiens Kidney (Srivastava)


text: live

text: Generic

text: Homo sapiens

text: Pancreas

text: Homo sapiens Pancreas (Srivastava)


text: live

text: Generic

text: Homo sapiens

text: Fetal lung

text: Homo sapiens Fetal lung (Srivastava)


text: Shizuoka University

text: live

text: Generic

text: Acropora tenuis

text: full polyps

text: Acropora tenuis full polyps juveniles approximately 2 weeks after metamorphosis


text: Virginia State University

text: live

text: Generic

text: genotype #20

text: Phaseolus acutifolius

text: leaf


text: Cardiff University

text: live

text: Generic

text: interaction with Stereum gausapatum

text: Trametes versicolor

text: mycelium

text: Trametes versicolor reverse-subtracted


text: Cardiff University

text: live

text: Generic

text: interaction with Stereum gausapatum

text: Trametes versicolor

text: mycelium

text: Trametes versicolor forward-subtracted


text: Rongchang Campus, Southwest University

text: live

text: Generic

text: The SMARTTM cDNA Library Construction Kit (Clontech, USA cat. #634901) was used for the construction of the female adult Toxocara canis cDNA library. 1ug RNA was used with 1ul of SMART IV oligonucleotide (Clontech, USA) and 1ul of CDS III/3 PCR primer for first-strand cDNA synthesis. The double-stranded cDNA (ds-cDNA) was synthesized by long distance PCR (LD-PCR). The ligation products werecloned into the TriplEx2 phage vector, using Gigapack III Gold Packaging Extract from Stratagene. Then the mixtures were transformed into Escherichia coli XL-1-Blue host cell culture.

text: adult

text: dog

characteristic: lab-host

text: Toxocara canis

text: female

text: Rongchang

text: whole body

text: female adult Toxocara canis, Clontech SMARTTM cDNA Library Construction Kit


text: University of Oregon

characteristic: INSDC center name

text: live

text: Generic

text: day 5 post-fertilization

text: Danio rerio

text: ABC

text: whole embryo

text: zebrafish larvae day 5 post-fertlization library


text: University of Minnesota

text: live

text: Generic

text: Mandibular BA1 tissue was isolated from E10.5 embryos, either wild type (W) or Twsg1-/- of varying severity: unaffected (A) or severely affected(C). RNA for reverse transcription and RACE was isolated from pools of 5-7 arches.

text: embryonic day 10.5

text: Mus musculus

text: first branchial arch

text: 5' RACE for Cyp26a1 of WT & varied Twsg1-/- embryos


text: Institute of Botany, Chinese Academy of Sciences, Beijing

text: live

text: Generic

text: Salicornia europaea

text: shoot

text: Salicornia europaea shoot cDNA


text: DOE Joint Genome Institute

text: live

text: Generic

text: Carthage

characteristic: cultivar

text: The library was made from oligo(dT)-primed cDNA and cloned into vector pDNR-LIB (Clontech Laboratories, Inc., Mountain View, CA) using the SMART system (BD Biosciences, San Jose, CA). To generate the first strand, polyA RNA was primed with an oligo(dT) primer (5'-ATTCTAGAGGCCGAGGCGGCCGACATG(T)30 VN-3', where V = A, G, or C). The second strand was synthesized by PCR using primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' followed by digestion of the SfiI sites. Normalized library was created and sequencing performed by the DOE Joint Genome Institute (Walnut Creek, CA).

text: Panicum virgatum

text: SfiIA

text: SfiIB

text: leaf

text: CCTW Panicum virgatum Carthage normalized leaf

text: pDNR-LIB

characteristic: vector


characteristic: External Id

text: DOE Joint Genome Institute

characteristic: INSDC first public

text: live

text: Generic

text: Cave-in-Rock

text: Panicum virgatum

text: SfiIA

text: SfiIB

text: leaf

text: CCTW Panicum virgatum Cave-in-Rock normalized leaf

text: pDNR-LIB


text: DOE Joint Genome Institute

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Kanlow

text: Panicum virgatum

text: SfiIA

text: SfiIB

text: leaf

text: CCTY Panicum virgatum Kanlow normalized leaf

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: Pathfinder

text: Panicum virgatum

text: SfiIA

text: SfiIB

text: leaf

text: CCTZ Panicum virgatum Pathfinder normalized leaf

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: Dacotah

text: Panicum virgatum

text: SfiIA

text: SfiIB

text: leaf

text: CFBY Panicum virgatum Dacotah normalized leaf

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: Forestburg

text: Panicum virgatum

text: SfiIA

text: SfiIB

text: leaf

text: CFBZ Panicum virgatum Forestburg normalized leaf

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: Sunburst

text: Panicum virgatum

text: SfiIA

text: SfiIB

text: leaf

text: CFCA Panicum virgatum Sunburst normalized leaf

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: Panicum virgatum

text: SfiIA

text: SfiIB

text: leaf

text: CFCB Panicum virgatum WS4U normalized leaf

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: Panicum virgatum

text: SfiIA

text: SfiIB

text: leaf

text: CFHA Panicum virgatum K101 normalized leaf

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: Panicum virgatum

text: SfiIA

text: SfiIB

text: leaf

text: CFHB Panicum virgatum U418 normalized leaf

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: Panicum virgatum

text: SfiIA

text: SfiIB

text: leaf

text: CFHC Panicum virgatum U518 normalized leaf

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: Panicum virgatum

text: SfiIA

text: SfiIB

text: leaf

text: CFHF Panicum virgatum U601 normalized leaf

text: pDNR-LIB


text: School of Life Sciences, Lanzhou University, China

text: live

text: Generic

text: Rheum alexandrae

text: hermaphrodite

text: bracts; leaves

text: Bracts and Leaves of Rheum alexandrae Library


text: Central Tasar Research and Training Institute

text: live

text: Generic

text: Antheraea mylitta male accessory gland specific gene

text: adults (moths)

characteristic: dev-stage

text: accessory gland

characteristic: organ

text: Antheraea mylitta

text: male

text: Daba bivoltine

text: accessory gland tissue

text: Antheraea mylitta male accessory gland tissue from adults


text: Rongchang Campus, Southwest University

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: The SMARTTM cDNA Library Construction Kit (Clontech, USA) was used for the construction of the male adult Toxocara canis cDNA library. 1ug RNA was used with 1ul of SMART IV oligonucleotide (Clontech, USA) and 1ul of CDS III/3' PCR primer for first-strand cDNA synthesis. The double-stranded cDNA (ds-cDNA) was synthesized by long distance PCR (LD-PCR). The ligation products werecloned into the TriplEx2 phage vector, using Gigapack III Gold Packaging Extract from Stratagene. Then the mixtures were transformed into Escherichia coli XL-1-Blue host cell culture.

text: adult

text: dog

text: Toxocara canis

text: male

text: Rongchang

text: whole body

text: male adult Toxocara canis, Clontech SMARTTM cDNA Library Construction Kit


text: University of Maryland - College Park

text: live

text: Generic

text: Culture harvested from various time points during the day and across the life cycle. No sexual stage was seen for this strain.

text: all except for sexual stages

text: Chlorokybus atmophyticus

characteristic: organism

text: UTEX 2591

text: Chlorokybus atmophyticus EST library

characteristic: title


text: University of Maryland - College Park

text: live

text: Generic

characteristic: description

text: all except for sexual stages

text: Klebsormidium flaccidum

text: UTEX 321

text: Klebsormidium flaccidum EST library


text: University of Maryland - College Park

text: live

text: Generic

text: Culture harvested from various time points during the day and across the life cycle.

text: all stages

text: Nitella hyalina

characteristic: strain

text: Nitella hyalina EST library


text: University of Maryland - College Park

text: live

text: Generic

text: all except for sexual stages

text: Penium margaritaceum

text: SKD-2004, CL-18

text: Penium margaritaceum EST library


text: University of Maryland - College Park

text: live

text: Generic

text: all except for sexual stages

text: Chaetosphaeridium globosum

text: SAG 26.98

text: Chaetosphaeridium globosum EST library


text: Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences

text: live

text: Generic

text: Cyprinus carpio

text: male & female

characteristic: sex

text: brain

characteristic: tissue-type

text: Carp brain tissue cDNA Library


text: CNRS - Universite Paris-Sud

characteristic: INSDC center name

text: live

text: Generic

text: Exponential growth culture

text: Bodo saltans

text: SCCAP BS364

text: Bodo saltans exponential growth


text: CNRS - Universite Paris-Sud

text: live

text: Generic

text: Exponential growth culture

text: Procryptobia sorokini

text: ELara

text: Procryptobia sorokini exponential growth


text: CNRS - Universite Paris-Sud

text: live

text: Generic

text: Exponential growth culture

text: Rhynchomonas nasuta

text: ELara

text: Rhynchomonas nasuta exponential growth


text: Agricultural University of Hebei

text: live

text: Generic

text: Young roots

characteristic: cell type

text: Total RNA was extracted by Trizol, reverse transcription was conducted by AMV reverse transcriptase; then double strand cDNA was synthesized and subjected to SSH analysis by using PCR Select cDNA Subtraction method (Clontech company) cDNA from the low phosphorus stress roots used as the tester, cDNA from the control (no-stress) used as the driver, after twice hybridization the PCR products were purified and cloned into pGM-T vector and transformed to E coli strain DH5alpha, sequencing were conducted by T7 primer.

text: Root materials collected at three-leaf stage, after 1 hr, 3 hr, 6 hr, 12 hr, 24 hr and 48 hr low phosphorus stress in Baoding of Hebei

text: Glycine max

text: Roots

text: SSH-cDNA library of low phosphorus stress in roots of high efficient phosphorus variety Zhonghuang15 compared with no stress


text: Centre for Genetic Engineering and Biotechnology

text: live

text: Generic

text: larvae

text: gut

text: Plutella xylostella

text: Karak

text: epithelium

text: Bt-resistant Plutella xylostella midgut epithelium ESTs library


text: Faculty of Medicine, University of Miyazaki, Miyazaki

text: live

text: Generic

text: infective third stage larvae

text: Strongyloides venezuelensis

text: Strongyloides venezuelensis infective third stage larvae library


text: Alfred-Wegener-Institut fur Polar- und Meeresforschung

text: live

text: Generic

text: Including ESTs from different physiological conditions: non-sressed, different light intensities, N-limitation, different timepoints during photocycle

text: Alexandrium ostenfeldii

text: Alexandrium ostenfeldii EST library


text: INRA - UMR BIOGECO

text: live

text: Generic

text: The cDNA library was made using mRNA extracted from swelling buds harvested on 3 adult European beech located in the Ciron Valley (France). The library was built using the Clone Miner cDNA library construction kit (Invitrogen) according to the manufacturers instructions

text: swelling buds

text: Fagus sylvatica

text: bud

text: WZOAFSAA

characteristic: vector


characteristic: External Id

text: University of Trieste

text: live

text: Generic

text: Dicentrarchus labrax

text: head kidney

text: Dicentrarchus labrax head kidney


text: Bose Institute

characteristic: INSDC first public

text: live

text: Generic

text: himsagar

characteristic: cultivar

text: According to the instructions given in Clontech Manual on SMART cDNA Library Construction Kit Manual (No. 634901; Clontech Lab Inc, USA)

text: Mangifera indica

text: ripe fruit

text: Ripe fruit library of Mangifera indica L. (cv himsagar)


text: UMCG Groningen

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Cultured epithelial cells

text: PCDH1 5'RACE (Invitrogen, RLM-RACE) performed on cultured bronchial epithelial cells of asthma patients. PCR-products were cloned in pCR-TOPO 4.1, and sequenced using M13Rev primer.

text: Passage 3

text: Human Lung

text: Homo sapiens

text: Airway epithelium

text: PCDH1 5'RACE


text: UMCG Groningen

text: live

text: Generic

text: Cultured epithelial cells

text: PCDH1 isoform 2 3'RACE (Invitrogen, RLM-RACE) performed on cultured bronchial epithelial cells of asthma patients. PCR-products were cloned in pCR-TOPO 4.1, and sequenced using M13Rev primer.

text: Passage 3

characteristic: dev-stage

text: Human Lung

characteristic: organ

text: Homo sapiens

text: Airway epithelium

text: PCDH1 3'RACE isoform 2


text: Suleyman Demirel University

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Rubidoux

text: Seedling 1 year old

text: E.coli JM109

characteristic: lab-host

text: Citrus trifoliata

text: Leaf

text: Four Hour Cold-induced subtractive cDNA library

text: pGEM-Teasy


text: University of Saskatchewan

text: live

text: Generic

text: The cDNA library was prepared from leaftlet of canadian lentil cultivar 'Eston' inoculated with Colletotrichum truncatum CT-21.

characteristic: description

text: Hemibiotrophic switch

text: Laboratory host

text: Lens culinaris/Colletotrichum truncatum mixed EST library

characteristic: organism

text: In planta Colletotrichum truncatum CT-21 secretome

characteristic: title

text: Plasmid

text: pBluescript II SK(+) XR


text: The Biomedical Center

text: live

text: Generic

text: cDNA Library was constructed using Marathon(TM) cDNA Amplification Kit (Clontech).

text: adult

text: lung

text: Homo sapiens

text: squamous cell carcinoma

characteristic: tissue-type

text: Homo sapiens primary lung tissue (Polev)

text: plasmid

text: pGEM-T Easy Vector


text: The Biomedical Center

characteristic: INSDC center name

text: live

text: Generic

text: cDNA Library was constructed using SMART RACE cDNA Amplification Kit (Clontech).

text: adult

text: ovary

text: Homo sapiens

text: carcinoma

text: Homo sapiens primary ovary tissue (Polev)


text: The Biomedical Center

text: live

text: Generic

text: placenta

text: Homo sapiens

text: Homo sapiens placenta (Polev)


text: The Biomedical Center

text: live

text: Generic

text: adult

text: uterus

text: Homo sapiens

text: moderately differentiated endometrium adenocarcinoma

text: Homo sapiens primary uterus tissue (Polev)

text: plasmid

text: pGEM-T Easy Vector


text: Academia Sinica

text: live

text: Generic

text: description

text: 1 month larvae

text: Epinephelus coioides

text: hermaphrodite

characteristic: sex

text: whole larvae

text: Orange-spotted grouper 1 month larvae Library


text: Guizhou Tobacco Research Institute

text: live

text: Generic

text: Nicotiana tabacum

text: leaf and flower

text: Normalized cDNA Library Tobacco Leaves and Flowers


text: National Research Center on Plant Biotechnology,

text: live

text: Generic

text: Identification of differntially expressed EST during TDZ induced shoot morphogenesis in chickpea

text: In vitro stage (about 15 days old)

text: Cicer arietinum

text: TDZ treated Chickpea SSH library

text: pGEM-T EASY

characteristic: vector


characteristic: External Id

text: National Institute of Allergy and Infectious Diseases

text: live

text: Generic

text: Unidirectional library was constructed using long distance PCR (Smart cDNA kit) and Lambda TriplEx2 vector

text: One first instar, 3 adults males and 3 adult females were used

text: Collected in Limao Verde, MT, Brazil on April 2009

characteristic: isolate

text: salivary glands

text: Triatoma matogrossensis

text: Salivary gland cDNA library T_mat_sg1

text: Phage

text: Lambda TriplEx2


text: Suleyman Demirel University

characteristic: INSDC first public

text: live

text: Generic

text: description

text: Seedling 1 year old

text: E.coli JM109

text: Citrus limonia

text: Leaf

text: Subtractive cDNA library of 30-day salinity stressed Rangpur lime

text: pGEM-Teasy


text: Suleyman Demirel University

characteristic: INSDC last update

text: live

text: Generic

text: description

text: Seedling 1 year old

text: E.coli JM109

text: Citrus limonia

text: Leaf

text: Subtractive cDNA library of 14-day drought stressed Rangpur lime

text: pGEM-Teasy


text: Instituto de Investigaciones Marinas (IIm), CSIC

text: live

characteristic: INSDC status

text: Generic

text: Mytilus galloprovincialis

text: Hemocytes

text: Vibrio anguillarum treated mussel hemocytes cDNA library


text: live

text: Generic

text: Mytilus galloprovincialis

text: Hemocytes

text: PBS treated mussel hemocytes cDNA library


text: live

text: Generic

text: Mytilus galloprovincialis

text: Hemocytes

text: Micrococcus lysodeikticus treated mussel hemocytes cDNA library


text: University of Tuscia

text: live

text: Generic

text: Olea europaea

text: flower

text: Olea europaea flower


text: Universiti Kebangsaan Malaysia

text: live

text: Generic

text: This research was done in INBIOSIS, UKM, Malaysia with the collaboration of National Institute of Crop Science, RDA, Suwon, Korea through the student internship program. The research here focuses mainly on the construction cDNA-AFLP transcripts profiling of Methyl jasmonate-elicited Polygonum minus. By cDNA-AFLP analysis, the expression profiles of 100¦ÌM elicited Polygonum minus was compared with the control plants. Total RNA from treated and control Polygonum minus¡¯s leaves were extracted using the modified Lopez-Gomez and Gomez Lim, 1992 method. The total RNA was then subjected to mRNA purification using PolyATtract mRNA Isolation Kit. cDNAs were synthesized and AFLP was performed by using the AFLP Expression Analysis Kit (Li-Cor Biosciences, USA) with 64 different primer combinations. From the cDNA-AFLP based transcript profiling obtained, trasncript-derived fragments that showed up-regulated patterns in the treated plants as compared to the controlled plants were identified, excised from the gel, reamplified and cloned into pGEM-T Easy Vectors before transforming into E. coli Top 10 cells. Plasmids were extracted, checked for inserts through colony PCR before sending for sequencing.

text: E. coli strain TOP 10

text: Leaves

text: Persicaria minor

text: Methyl Jasmonate-treated x non-treated Polygonum minus leaves cDNA-AFLP profiles library

text: pGEM-T Easy Vector


text: Ocean University of China

text: live

text: Generic

text: Ulva prolifera

text: Ulva prolifera responsible for green tide in China


text: Institute of Marine Research

text: live

text: Generic

text: Adult

characteristic: dev-stage

text: Lepeophtheirus salmonis

text: Lepeophtheirus salmonis SB


text: Institute of Marine Research

text: live

text: Generic

text: Colon/intestine adult

text: Lepeophtheirus salmonis

text: Lepeophtheirus salmonis FB


text: Institute of Marine Research

text: live

text: Generic

text: Adult with blood

text: Lepeophtheirus salmonis

text: Lepeophtheirus salmonis ME


text: Institute of Marine Research

text: live

text: Generic

text: T1 adult

text: Lepeophtheirus salmonis

text: Lepeophtheirus salmonis L1T


text: Institute of Marine Research

text: live

text: Generic

text: Preadult II

text: Lepeophtheirus salmonis

text: Lepeophtheirus salmonis PU


text: Institute of Marine Research

text: live

text: Generic

text: With filament, preAI, preAII

text: Lepeophtheirus salmonis

text: Lepeophtheirus salmonis LF


text: Institute of Marine Research

text: live

text: Generic

text: Preadult stages

text: Lepeophtheirus salmonis

text: Lepeophtheirus salmonis LPU


text: Institute of Marine Research

text: live

text: Generic

text: Preadult stages

text: Lepeophtheirus salmonis

text: Lepeophtheirus salmonis LPA


text: Institute of Marine Research

text: live

text: Generic

text: Lepeophtheirus salmonis

text: Lepeophtheirus salmonis CC


text: Institute of Marine Research

text: live

text: Generic

text: Nauplius

text: Lepeophtheirus salmonis

text: Lepeophtheirus salmonis NA


text: Institute of Marine Research

text: live

text: Generic

text: Adult

text: Lepeophtheirus salmonis

text: Lepeophtheirus salmonis HA


text: Institute of Marine Research

text: live

text: Generic

text: PreAI/II, adult, with filament

text: Lepeophtheirus salmonis

text: Lepeophtheirus salmonis LNO


text: Institute of Marine Research

text: live

text: Generic

text: Copepodite

text: Lepeophtheirus salmonis

text: Lepeophtheirus salmonis LNC


text: Institute of Marine Research

text: live

text: Generic

text: Egg string

text: Lepeophtheirus salmonis

text: Lepeophtheirus salmonis NLG


text: Institute of Applied Science and Technology (ICAT)

text: live

text: Generic

text: Regent and Trincadeira

characteristic: cultivar

text: The cDNA library was constructed through a combination of the SMART library construction and the Gateway method. cDNA sequences were clones into pDNORTM221 Gateway vector.

text: E.coli

text: Leaf

characteristic: organ

text: Vitis vinifera

text: Leaf

text: Vitis vinifera 'Regent' and 'Tricadeira' SMART-GATEWAY combined cDNA library

text: plasmid


text: INRA - UMR BIOGECO

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: meristematic cells

characteristic: cell type

text: The cDNA library was made using mRNA extracted from meristematic cells harvested using laser capture microdissection from apical bud. Meristematic cells were sampled from one genotype located at pierroton forestry station in France on January 2007 (quiescent stage). The library was built using the CreatorTM SmartTM cDNA library construction kit (clontech) according to the manufacturer's instructions

text: Quercus robur

text: apical bud

text: pDNR-LIB


text: INRA - UMR BIOGECO

text: live

text: Generic

text: The composite cDNA library was made using mRNA extracted from setting and swelling buds harvested on 3 adult European ashes located in the Ciron Valley (France). The library was built using the CreatorTM SmartTM cDNA library construction kit (Clontech) according to the manufacturer's instructions.

characteristic: description

text: adult

text: Fraxinus excelsior

characteristic: organism

text: quiescent and swelling buds

text: pDNR-LIB


text: INRA - UMR BIOGECO

text: live

text: Generic

text: Library was made using Suppression Subtractive Hybridization method (Diatchenko et al., 1996) from adult leaves collected on a subset of individuals segregating for the unfavourable alleles at a strong QTL for water use efficiency. A mixture of genotypes was used.

text: one year old cuttings

text: Quercus robur

text: green leaves

characteristic: tissue-type

characteristic: title

text: Pgmt easy


text: IFAPA Centro Alameda del Obispo

characteristic: INSDC center name

text: live

text: Generic

text: Cucurbita pepo

text: leaves

text: EST cloned from Cucurbita pepo leaves


text: Cornell University

text: live

text: Generic

text: Tissues were collected from 10 adult Brienomyrus brachyistius whole electric organs (removed from the caudal peduncle) and trunk skeletal muscle (approx. 2cm x 1 cm x 0.5cm), posterior to operculum, superior to lateral line; skin removed). Tissue was immediately frozen in liquid nitrogen, pulverized using mortar and pestle, and transferred to TRIZOL solution (Invitrogen). Total RNA was isolated following the manufacturer's instructions method, and then stored at -80 C in aliquots until use. Total RNA was PolyA+ purified using a FastTrack MAG mRNA isolation kit (Invitrogen). polyA+ RNA was reverse transcribed to cDNA using SuperScript III RT (Invitrogen). Subtractive hybridization was then performed following the method of Rebrikov (2003), using EO as the tester and SM as the driver (EO library). A second library was also constructed using SM as the tester with EO as the driver (SM library). These SSH protocols resulted in two pools of PCR products representing mRNAs enriched in EO and SM respectively. PCR products from EO and SM libraries were subsequently cloned into chemically competent E. coli using the TOPO TA cloning system (Invitrogen), resulting in many hundreds of colonies for each library. 130 Colonies from the EO library and 36 colonies from the SM library were randomly selected for sequencing. These colonies were grown overnight in LB broth containing 50µg/ml ampicillin. Overnight colonies were individually miniprepped using the Invitrogen PureLink miniprep kit. Isolated and purified DNA was then sequenced from the T7 promoter at the Cornell Core Laboratory Services with an Automated 3730 DNA Analyzer (Applied Biosystems), using Big Dye Terminator chemistry (Applied Biosystems) and AmpliTaq-FS DNA Polymerase.

text: Electric Organ

text: Brienomyrus brachyistius

text: B. brachyistius electric organ subtracted library using skeletal muscle driver

text: Plasmid

text: pCR 2.1-TOPO

characteristic: vector


characteristic: External Id

text: Cornell University

text: live

text: Generic

text: Tissues were collected from 10 adult Brienomyrus brachyistius whole electric organs (removed from the caudal peduncle) and trunk skeletal muscle (approx. 2cm x 1 cm x 0.5cm), posterior to operculum, superior to lateral line; skin removed). Tissue was immediately frozen in liquid nitrogen, pulverized using mortar and pestle, and transferred to TRIZOL solution (Invitrogen). Total RNA was isolated following the manufacturer's instructions method, and then stored at -80 C in aliquots until use. Total RNA was PolyA+ purified using a FastTrack MAG mRNA isolation kit (Invitrogen). polyA+RNA was reverse transcribed to cDNA using SuperScript III RT (Invitrogen). Subtractive hybridization was then performed following the method of Rebrikov (2003), using EO as the tester and SM as the driver (EO library). A second library was also constructed using SM as the tester with EO as the driver (SM library). These SSH protocols resulted in two pools of PCR products representing mRNAs enriched in EO and SM respectively. PCR products from EO and SM libraries were subsequently cloned into chemically competent E. coli using the TOPO TA cloning system (Invitrogen), resulting in many hundreds of colonies for each library. 130 Colonies from the EO library and 36 colonies from the SM library were randomly selected for sequencing. These colonies were grown overnight in LB broth containing 50µg/ml ampicillin. Overnight colonies were individually miniprepped using the Invitrogen PureLink miniprep kit. Isolated and purified DNA was then sequenced from the T7 promoter at the Cornell Core Laboratory Services with an Automated 3730 DNA Analyzer (Applied Biosystems), using Big Dye Terminator chemistry (Applied Biosystems) and AmpliTaq-FS DNA Polymerase.

text: Skeletal Muscle

text: Brienomyrus brachyistius

text: B. brachyistius skeletal muscle subtracted library using electric organ driver

text: Plasmid

text: pCR 2.1-TOPO


text: Universite Laval

text: live

text: Generic

text: The fifth and sixth leaves from the index leaf were taken from at least five different trees and surface sterilized in a 1% household bleach solution for 5 min and then washed twice in sterile water. Disks (diameter, 2 cm) were inoculated on the abaxial side with M. medusae f. sp. deltoidae at a density of 1,000 spores (in 0.01% Tween) per cm2 with a Crown spray tool. After inoculation, the disks were kept on wet paper in large Parafilm-sealed petri dishes and incubated for 8 days in a growth chamber at 18°C with a long photoperiod. Procedure is fully described in Boyle (Boyle et al., AEM 71:1546, 2005). cDNA was prepared from 5 ng of poly A+ selected RNA and was directionally ligated into the pBluescript II SK (+) XR vector (Stratagene), transformed by electroporation into DH10B cells (In vitrogen) for propagation.

text: E. coli DH10B cells

characteristic: lab-host

text: Leaves

text: Populus x jackii

text: Eco-RI

text: Xho-I

text: Male

characteristic: sex

text: Jackii

characteristic: strain

text: Melampsora medusae infected Leaves (compatible interaction)

text: GQ014 - 8-day M.medusae infected leaves

text: pBluescript II SK (+) XR


text: USDA-ARS-JWDSRC

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Description

text: Heliothis virescens

text: Lab strain

text: Midgut

text: Heliothis virescens larval midgut library

text: DNR-Lib


text: INRA - UMR BIOGECO

text: live

text: Generic

text: This non-normalized cDNA library was made using mRNA extracted from developing phellem sampled on the inner side of regeneration cork boards collected on ten 60-70 years-old cork-trees at Herdade do Rosal (Montemor-o- Novo, Portugal). The library was built using the CreatorTM SmartTM cDNA library construction kit (Clontech) according to the manufacturer's instructions.

text: developing phellem

characteristic: dev-stage

text: Quercus suber

text: phellem

text: pDNR-LIB


text: Huazhong University of Science and Technology

text: live

text: Generic

characteristic: NCBI submission model

text: description

text: E.coli BM25.8

text: Dunaliella salina

text: SfilA

text: SfilB

text: Salt-tolerant Dunaliella Salina cDNA library 2

text: pTriplEx


text: Facultad de Quimica, Universidad Nacional Autonoma de Mexico

text: live

text: Generic

characteristic: NCBI submission package

text: Short oligonucleotides for pyrophosphatase sequences were used to amplify cDNA from common bean and cloned in pGEM-T-Easy (Promega). The sequences were extended with 3' RACE following standard protocols provided by manufacturer (Invitrogen).

text: Phaseolus vulgaris

text: Pinto Villa

text: Common been RACE small cDNA Library


text: USDA-ARS-Plant Sciences Institute

text: live

text: Generic

text: somatic

characteristic: cell type

text: Solexa sequencing of total RNA from Moniliophthora roreri mycelium grown in liquid culture

text: Theobroma cacao L.

text: Moniliophthora roreri

text: mycelium

text: M. roreri-mycelium


text: University of Liege, Gembloux Agro-Bio Tech

text: live

text: Generic

characteristic: cultivar

text: Sequence obtained by cDNA-AFLP

characteristic: description

text: Prunus persica

text: hermaphrodite

text: Leaves

text: PLMVd-infected GF-305 peach tree leaves


text: Samuel Roberts Noble Foundation

text: live

text: Generic

text: The library was prepared by following the instruction of user manual of the PCR-selected cDNA subtraction kit (Clontech). Briefly, The tester and driver ds cDNA were prepared from the two mRNA samples from root tissues of kudzu plants. The tester and driver cDNA were separately digested with RsaI and then the digested tester cDNA was ligated to different adaptor but the driver cDNA had no adaptor ligation. The tester and driver cDNA was subjected to first and second hybridization which generated differentially expressed sequences for PCR amplification. The PCR amplified products were ligated to pDNR-LIB vector for E.coli transformation. The transformed colonies were picked up and cultured and used for DNA sequencing.

text: Pueraria montana var. lobata

characteristic: organism

text: Roots

characteristic: tissue-type

text: Kudzu subtraction cDNA library

text: Cloning vector

text: pDNR-LIB


text: live

text: Generic

text: E.coli XL1-Blue

text: Dunaliella salina

text: SfilA

text: SfilB

text: Salt-tolerant Dunaliella Salina cDNA library (3)

characteristic: title


text: IFAPA Centro Alameda del Obispo

characteristic: INSDC center name

text: live

text: Generic

text: Sulla coronaria

text: EST cloned from Hedysarum coronarium leaves


characteristic: External Id

text: Bose Institute

text: live

text: Generic

text: PCR-select cDNA subtraction using leaf as driver and anther as tester. pGEM®-T Easy vector was used.

text: 3 days old bud

text: Sesamum indicum

text: Anther

text: Sesame anther specific library


text: National Yang-Ming University

text: live

text: Generic

text: Diploid

characteristic: cell-line

text: The cDNA library of Ganoderma lucidum BCRC 36123 (GLEST01, dikaryon) was constructed by ZAP Express cDNA Synthesis Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV harboring the inserted cDNA from Ganoderma lucidum BCRC 36123 was single clone or mass excised from the Lambda phage, with the helper phage co-infected bacterial host XLI-Blue MRF'. The released circularized, packed phagemid particles after infection and multiplication in XLOLR bacterial host were plated on a Q-tray, picked by robots by white-blue colony selection, first stored in 384-well microtiter plates, and further multiplied in 96-well plates for DNA extraction and subsequent sequencing. Colony PCR was performed by T3, T7 primers to prepare templates for sequencing from both ends. Sequences were obtained from trace files by base calling with phred 0.020425.c while setting the value of the phred parameter '-trim_cutoff' as 0.01. EMBOSS vectorstrip and BLAST of EST sequences against NCBI UniVec database were applied to remove vector and adaptor sequences. BLAST of EST sequences against all reference sequences of microbial, viral and mitochondrial genomes from NCBI ftp site was applied to remove suspicious contamination of sequences (sequences with blast hit whose E-value is less than 1e-20 will be regarded as contamination). Only those with more than ten contigious A/T will be regarded as poly-A/T tracks.

text: 5 days

text: E. coli XLOLR

characteristic: lab-host

text: Ganoderma lucidum

text: BCRC 36123; ATCC32471

text: Mycelium

text: Phagemid

text: pBK-CMV

characteristic: vector


text: National Yang-Ming University

characteristic: INSDC first public

text: live

text: Generic

text: Diploid

text: The cDNA library of Ganoderma lucidum BCRC 36123 (GLEST02, dikaryon) was constructed by ZAP Express cDNA Synthesis Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV harboring the inserted cDNA from Ganoderma lucidum BCRC 36123 was single clone or mass excised from the Lambda phage, with the helper phage co-infected bacterial host XLI-Blue MRF'. The released circularized, packed phagemid particles after infection and multiplication in XLOLR bacterial host were plated on a Q-tray, picked by robots by white-blue colony selection, first stored in 384-well microtiter plates, and further multiplied in 96-well plates for DNA extraction and subsequent sequencing. Colony PCR was performed by T3, T7 primers in 384-well format to prepare templates for sequencing, and sequencing was performed in 96-well format by the T3 primer only. Sequences were obtained from trace files by base calling with phred 0.020425.c while setting the value of the phred parameter '-trim_cutoff' as 0.01. EMBOSS vectorstrip and BLAST of EST sequences against NCBI UniVec database were applied to remove vector and adaptor sequences. BLAST of EST sequences against all reference sequences of microbial, viral and mitochondrial genomes from NCBI ftp site was applied to remove suspicious contamination of sequences (sequences with blast hit whose E-value is less than 1e-20 will be regarded as contamination). Only those with more than ten contigious A/T will be regarded as poly-A/T tracks.

text: 18 days

text: E. coli XLOLR

text: Ganoderma lucidum

text: BCRC 36123; ATCC32471

characteristic: strain

text: Mycelium

text: Phagemid

text: pBK-CMV


text: National Yang-Ming University

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Diploid

text: The cDNA library of Ganoderms lucidum BCRC 36123 (GLEST03, dikaryon) was constructed by ZAP Express cDNA Synthesis Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV harboring the inserted cDNA from Ganoderma lucidum BCRC 36123 was single clone or mass excised from the Lambda phage, with the helper phage co-infected bacterial host XLI-Blue MRF'. The released circularized, packed phagemid particles after infection and multiplication in XLOLR bacterial host were plated on a Q-tray, picked by robots by white-blue colony selection, first stored in 384-well microtiter plates, and further multiplied in 96-well plates for DNA extraction and subsequent sequencing. Colony PCR was performed by T3, T7 primers in 384-well format to prepare templates for sequencing, and sequencing was performed in 96-well format by the T3 primer only. Sequences were obtained from trace files by base calling with phred 0.020425.c while setting the value of the phred parameter '-trim_cutoff' as 0.01. EMBOSS vectorstrip and BLAST of EST sequences against NCBI UniVec database were applied to remove vector and adaptor sequences. BLAST of EST sequences against all reference sequences of microbial, viral and mitochondrial genomes from NCBI ftp site was applied to remove suspicious contamination of sequences (sequences with blast hit whose E-value is less than 1e-20 will be regarded as contamination). Only those with more than ten contigious A/T will be regarded as poly-A/T tracks.

text: 30 days

text: E. coli XLOLR

text: Ganoderma lucidum

text: BCRC 36123; ATCC32471

text: Mycelium

text: Phagemid

text: pBK-CMV


text: National Yang-Ming University

text: live

text: Generic

characteristic: NCBI submission model

text: Diploid

text: The cDNA library of Ganoderma lucidum BCRC 36123 (GLEST04, dikaryon) was constructed by ZAP Express cDNA Synthesis Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV harboring the inserted cDNA from Ganoderma lucidum BCRC 36123 was single clone or mass excised from the Lambda phage, with the helper phage co-infected bacterial host XLI-Blue MRF'. The released circularized, packed phagemid particles after infection and multiplication in XLOLR bacterial host were plated on a Q-tray, picked by robots by white-blue colony selection, first stored in 384-well microtiter plates, and further multiplied in 96-well plates for DNA extraction and subsequent sequencing. Colony PCR was performed by T3, T7 primers in 384-well format to prepare templates for sequencing, and sequencing was performed in 96-well format by the T3 primer only. Sequences were obtained from trace files by base calling with phred 0.020425.c while setting the value of the phred parameter '-trim_cutoff' as 0.01. EMBOSS vectorstrip and BLAST of EST sequences against NCBI UniVec database were applied to remove vector and adaptor sequences. BLAST of EST sequences against all reference sequences of microbial, viral and mitochondrial genomes from NCBI ftp site was applied to remove suspicious contamination of sequences (sequences with blast hit whose E-value is less than 1e-20 will be regarded as contamination). Only those with more than ten contigious A/T will be regarded as poly-A/T tracks.

text: 14 days

characteristic: dev-stage

text: E. coli XLOLR

text: Ganoderma lucidum

text: BCRC 36123; ATCC32471

text: Mycelium

text: Phagemid

text: pBK-CMV


text: National Yang-Ming University

text: live

text: Generic

characteristic: NCBI submission package

text: Haploid

characteristic: cell type

text: The cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, monokaryon) was constructed by ZAP Express cDNA Synthesis Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV harboring the inserted cDNA from Ganoderma lucidum BCRC 37180 was mass excised from the Lambda phage, with the helper phage co-infected bacterial host XLI-Blue MRF'. The released circularized, packed phagemid particles after infection and multiplication in XLOLR bacterial host were plated on a Q-tray, picked by robots by white-blue colony selection, first stored in 384-well microtiter plates, and further multiplied in 96-well plates for DNA extraction and subsequent sequencing. Colony PCR was performed by T3, T7 primers in 384-well format to prepare templates for sequencing, and sequencing was performed in 96-well format by the T3 primer only. Sequences were obtained from trace files by base calling with phred 0.020425.c while setting the value of the phred parameter '-trim_cutoff' as 0.01. EMBOSS vectorstrip and BLAST of EST sequences against NCBI UniVec database were applied to remove vector and adaptor sequences. BLAST of EST sequences against all reference sequences of microbial, viral and mitochondrial genomes from NCBI ftp site was applied to remove suspicious contamination of sequences (sequences with blast hit whose E-value is less than 1e-20 will be regarded as contamination). Only those with more than ten contigious A/T will be regarded as poly-A/T tracks.

text: 18 days

text: E. coli XLOLR

text: Ganoderma lucidum

text: BCRC 37180

text: Mycelium

text: Phagemid

text: pBK-CMV


text: Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Science

text: live

text: Generic

text: the algal strain Microctinium spp.grown on TAP agar plate were inoculated into 100mL Erlenmeyer flasks with 50 mL of TAP medium, and maintained in an incubator shaker with 230rpm at 25¡æ for 3d.Then, the culture were collected and resuspended with 50 mL TAP medium and nitrogen-deficient medium, respectivly. The samples were collected at 72 h after nitrogen deficiency. The total RNA was extracted using Trizol method and Poly (A+) mRNA was isolated from the total RNA using OligotexTM-dT30 <super> mRNA purification Kit . Suppression subtractive hybridization was performed using Clontech PCR-selectTM cDNA Subtractive kit (Clontech) according to the manufacturer¡¯s instructions. cDNA from the cells grown in N starvation medium were considered as the tester, and cDNA from the cells under normal medium condition as the driver. Finally, the differentially expressed transcripts were cloned into PMD18-T vector. Sequencing was performed at Shanghai Sangon Biological Engineering Technology & Services Co., Ltd.

characteristic: description

text: Micractinium sp. HK002

text: 72-h nitrogen deficiency induced Micractinium spp. HK002 library


text: A.N.Belozersky Institute of Physico-Chemical Biology

text: live

text: Generic

text: mesophyll

text: Nicotiana benthamiana

characteristic: organism

text: leaf

text: Nicotiana benthamiana leaf mesophyll


text: University of Warwick

text: live

text: Generic

text: Calypso

characteristic: cultivar

text: ripe fruit

text: Fragaria x ananassa

text: receptacle

characteristic: tissue-type

text: Fragaria x ananassa receptacle ripe fruit


text: Bose Institute

characteristic: INSDC center name

text: live

text: Generic

text: 4 day post infection cDNA-AFLP differentially expressed fragments.

text: 18 day old seedlings

text: Cicer arietinum

text: Root

text: Differentially expressed genes in Cicer arietinum during incompatible interaction with Fusarium oxysporum f. sp. ciceris

characteristic: title

text: pGEM-T


characteristic: External Id

text: Agricultural Research Service, USDA

text: live

text: Generic

text: Cryptosporidium parvum

text: Beltsville-1 strain

text: Cryptosporidium parvum excysting oocysts subtractive cDNA library


text: The institute of crop science research,CAAS

text: live

text: Generic

text: SSH library was constructed for the root tissue of soybean.About one-week-old seedings when the first pair of leaves expanded fully were used in 1/2Hongland solutions containing 0.9% (W/V) NaCl or nothing for control.

text: Glycine max

text: root

text: SSH cDNA library induced by salt from soybean


text: The Chinese University of Hong Kong

text: live

text: Generic

text: Lentinula edodes

text: dikaryotic mycelium

text: Lentinula edodes dikaryotic mycelium EST contigs


text: Universidade Federal de Santa Catarina

text: live

text: Generic

text: Adult

text: Penaeus vannamei

text: Gill

text: WSSV infected Litoepenaeus vannamei library


text: National Institute of Agrobiological Sciences

text: live

text: Generic

text: from embryos 1 day after oviposition to newly hatched 1st instar larvae

text: Mamestra brassicae

text: whole body of eggs or larvae

text: Mamestra brassicae embryonic and 1st instar larval cDNA library


text: School of Biological Science and Medical Engineering, Southeast University

text: live

text: Generic

text: All c-DNA have been sequenced from two directions by M13 forward and reverse primers

text: Branchiostoma belcheri

text: Amphioxus (Branchiostoma belcheri) mixed Library from fertilized egg to adult

text: pGEM-T Easy (Cat#: A1360)


text: Soochow University

text: live

text: Generic

text: Escherichia coli JM109

characteristic: lab-host

text: Neocaridina denticulata

text: Neocaridina denticulata cDNA library

text: pGEM-T vector


text: Central Tasar Research and Training Institute

text: live

text: Generic

text: Diapause specific gene of Antheraea mylitta Drury

text: pupae of diapausing generation

text: Terminalia tomentosa (Asan)

text: brain

characteristic: organ

text: Antheraea mylitta

text: male/female

characteristic: sex

text: Daba bivoltine

text: brain tissues

text: Antheraea mylitta brain tissue from pupae of diapausing generation


text: Sun Yat-sen University

characteristic: INSDC first public

text: live

text: Generic

text: Talipariti tiliaceum

text: leaves

text: cDNA library of Hibiscus tiliaceus leaves


text: Universite d'Angers

characteristic: INSDC last update

text: live

text: Generic

text: Radrazz

text: mature leaves

text: Rosa hybrid cultivar

text: Rose mature leaves library


text: Universite d'Angers

text: live

characteristic: INSDC status

text: Generic

text: Radrazz

text: root

text: Rosa hybrid cultivar

text: Rose root library


text: College of Landscape Architecture, Northeast Forestry University

text: live

text: Generic

characteristic: NCBI submission model

text: cDNA library was derived from vegetative and reproductive shoot tips of Agapanthus praecox ssp. orientalis. cDNA synthesis was initiated using a oligo(dT) primer. Double-stranded cDNA was blunted, digested with EcoRI and MseI restriction endonuclease, ligated to EcoRI and MseI adaptors, using cDNA-AFLP to screen the different expression gene, and cloned into the pMD18-T simple vector.

text: Vegetative stage and reproductive stage

text: Escherichia coli DH5-alpha

text: Agapanthus praecox subsp. orientalis

text: EcoRV

text: Shoot tips

text: Agapanthus praecox ssp. orientalis flowering library

text: Plasmid

text: pMD18-T simple vector

characteristic: vector


text: Duke University

text: live

text: Generic

characteristic: NCBI submission package

text: Total RNAs from brain of normal 60-day old C57BL/6J mice were used to amplify the 5' end of Shank3 mRNA with the 5'/3' RACE Kit, 2nd Generation (Roche) based on the protocol supplied by the manufacturer. mRNA was reverse-transcribed with Shank3-specific reverse primers GSP1, then the cDNA was tailed with poly(A) and amplified using Shank3-specific reverse primers GSP2 and oligo(dT) anchor primer with high-fidelity DNA polymerase (Roche) under the following cycling parameters: 35 cycles of 94degC for 30 sec, 60degC for 30 sec, and 70degC for 1 min, followed by 70degC for 10 min. The amplification products were gel purified, cloned into pGEM-T Easy Vector (Promega), and inserts were sequenced.

text: post-natal day 60

characteristic: dev-stage

text: brain

text: Mus musculus

text: male

characteristic: strain

text: whole brain

text: Mouse post-natal day 60 brain 5'-RACE, variant transcript of Shank3


text: National Institute of Oceanography

text: live

text: Generic

text: Full length cloning of TCTP gene from F. indicus using RACE PCR.

characteristic: description

text: Adult

text: Penaeus indicus

characteristic: organism

text: Hepatopancreas

text: Vibrio harveyi challenged Fenneropenaeus indicus postlarvae cDNA library

text: TOPO


text: Embrapa Recursos Geneticos e Biotecnologia

text: live

text: Generic

text: As part of genolyptus project, mature, fully expanded leaves were collected from 4-year-old E. grandis trees located in commercial plantations of Veracel Celulose S.A. (Eunapolis, BA, Brazil). Total RNA was extracted from 100-200 mg of tissues previously pulverized in mortars with pestles and liquid nitrogen using the PureLink Plant RNA Reagent Kit (Invitrogen) according to the multi-miniprep protocol instructions of the manufacturer. Polyadenylated RNA was subsequently purified from total RNA using the Oligotex mRNA Mini kit (Qiagen). RNA purity and quality were checked by both agarose gel electrophoresis and spectrophotometric measurements at 260 and 280 nm. Complementary DNA libraries were constructed using 1-5 ug mRNA and kits of the Superscript Plasmid System with Gateway Technology for cDNA Synthesis and Cloning (Invitrogen) and instructions of the manufacturer. Eluted fractions of double-stranded cDNA were stored at -20 degC and further ligated into pSport1 (Invitrogen). Ligated products were transformed into Escherichia coli Electromax DH10B competent cells (Invitrogen) using a Gene Pulser ll Electroporation System (Bio-Rad).

text: Fully expanded, mature

text: Escherichia coli DH10B

text: Leaf

text: Eucalyptus grandis

text: SaII

text: NotI

text: Mature leaves (EUGR-ML)

text: plasmid


text: Ecole Normale Superieure de Lyon

text: live

text: Generic

text: Sample name: CAB-INFL Soil conditions: aquatic plant Harvesting date: 01/03/2003 Description: inflorescense tissues experimental condition: normal

text: flower stages up to anthesis

text: SOLR

text: Cabomba aquatica

text: EcoRI

text: XhoI

text: inflorescence

characteristic: tissue-type

text: CabInf

text: pBluescript II SK(-)


text: RIKEN Center for Developmental Biology

characteristic: INSDC center name

text: live

text: Generic

text: mixed segmentation stages

text: Parasteatoda tepidariorum

text: whole embryo

text: House spider embryo cDNA library


text: Kirin Holdings Company, Ltd.

text: live

text: Generic

text: 13 days old

text: Brassica oleracea var. viridis

text: whole seedlings

text: full-length kale cDNA library (seedlings)

characteristic: title


characteristic: External Id

text: Kirin Holdings Company, Ltd.

text: live

text: Generic

text: from bud to postanthesis stage

text: Brassica oleracea var. viridis

text: flowers

text: full-length kale cDNA library (flowers)


text: Kirin Holdings Company, Ltd.

text: live

text: Generic

text: 60 days old

text: Brassica oleracea var. viridis

text: true leaves

text: full-length kale cDNA library (leaves)


text: Kirin Holdings Company, Ltd.

text: live

text: Generic

text: early seed developmental stage

text: Brassica oleracea var. viridis

text: capsules

text: full-length kale cDNA library (capsules)


text: Kirin Holdings Company, Ltd.

text: live

text: Generic

text: Brassica oleracea var. viridis

text: full-length kale cDNA library


text: Huazhong Agricultural University

text: live

text: Generic

text: Forward and reverse libraries were constructed via Suppression Subtractive Hybridization (SSH) using Poncirus trifoliata leaves treated at 4degC and 25degC with mixed samplings from five time points (6h, 1d, 3d, 5d, 7d)

text: three-month old

text: DH 5alpha

characteristic: lab-host

text: Citrus trifoliata

text: RsaI

text: RsaI

text: leaf

text: Cold-stressed leaf SSH cDNA library of Poncirus trifoliata

text: pMD 18-T


text: Chaudhary Charan Singh University

text: live

text: Generic

text: HD2329 + Lr28

characteristic: cultivar

text: differentially regulated TDFs in incompatible interaction of wheat challenged with Puccinia triticina

text: The first leaf of seven-day old wheat seedlings

text: Triticum aestivum

text: Leaf

text: Transcript derived fragments (TDFs) from wheat leaves inoculated by leaf rust fungus

text: pGEM-T easy


text: Jeju National University

text: live

text: Generic

text: Juvenile with shell length 100 +/- 10mm

text: E.coli SOLR

text: Epinephelus bruneus

text: EcoR I

text: Xho I

text: Normalized Bacteria Challenged Epinephelus bruneus cDNA library

text: Uni-ZAP XR vector


text: College of Veterinary Medicine, Seoul National University

text: live

text: Generic

text: Smooth muscle

characteristic: cell type

text: Animals were anesthesized, inert gauze was inserted into the omental surface of the abdomen with aseptic measure. Omentum was collected at and placed for RNA extraction. Complementary DNA (cDNA) was prepared and thereby DD PCR and RACE PCR for the detection and synthesis of full length sequence.

text: Abdomen

characteristic: organ

text: Canis lupus familiaris

text: Male & Female

characteristic: sex

text: Beagle

text: Omentum

text: Canine Omentum Library


text: College of Horticulture, Northwest A&F University

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Pyrus x bretschneideri

text: young fruit

text: Red Mutant of zaosu pear Supression Substractive Hybridization cDNA Library

text: pGEM-T Easy Vector


text: Virginia State University

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Drought susceptible line; genotype #10

text: Phaseolus acutifolius

text: leaf


text: Virginia State University

text: live

text: Generic

text: Drought resistant line; genotype #15

text: Phaseolus acutifolius

text: leaf


text: Virginia State University

text: live

text: Generic

text: Challenegd with Colletotrichum gleosporoides; genotype TDa 95-328

text: Dioscorea alata

text: leaf


text: WageningenUR-Plant Breeding

text: live

text: Generic

text: ACCESSION: CGN19285; Baldus. First strand cDNA 1 ug of total RNA was treated with DNAse I (Invitrogen, amplification grade; 18068-015) followed by RT PCR (Invitrogen SuperScriptTM III First-Strand Synthesis System for RT-PCR; 18080-051) using random hexamer primers in a final reaction volume of 20 ul. Samples without SuperScriptTM III reverse transcriptase (minus RT PCR) were included as controls for the DNAse I treatment. A fraction of 2 ul of the cDNA was used as a template in a PCR reaction with alpha-gliadin specific primers (alpha1F: 5'atg aaR acM ttt cYc atc-3';alpha5R; 5'gtt agt acc gaa gat gcc-3'). Subsequently, the PCR products were cloned in pGEM-T Easy (Promega).

text: 14 days post-anthesis

characteristic: dev-stage

text: single plant from CGN19285

characteristic: isolate

text: E-coli; XL1-blue

text: Triticum aestivum

text: developing seed

text: Baldus wheat Developing seeds, 14 days post-anthesis

text: plasmid

text: pGEMt easy

characteristic: vector


text: WageningenUR-Plant Breeding

text: live

text: Generic

text: ACCESSION: CGN23549; Lavett. First strand cDNA 1 ug of total RNA was treated with DNAse I (Invitrogen, amplification grade; 18068-015) followed by RT PCR (Invitrogen SuperScriptTM III First-Strand Synthesis System for RT-PCR; 18080-051) using random hexamer primers in a final reaction volume of 20 ul. Samples without SuperScriptTM III reverse transcriptase (minus RT PCR) were included as controls for the DNAse I treatment. A fraction of 2 ul of the cDNA was used as a template in a PCR reaction with alpha-gliadin specific primers (alpha1F: 5'atg aaR acM ttt cYc atc-3';alpha5R; 5'gtt agt acc gaa gat gcc-3'). Subsequently, the PCR products were cloned in pGEM-T Easy (Promega).

characteristic: description

text: 14 days post-anthesis

text: single plant from CGN23549

text: E-coli; XL1-blue

text: Triticum aestivum

characteristic: organism

text: developing seed

characteristic: tissue-type

text: Lavett wheat Developing seeds, 14 days post-anthesis

text: plasmid

text: pGEMt easy


characteristic: External Id

text: Virginia State University

characteristic: INSDC center name

text: live

text: Generic

text: Challenged with Colletotrichum gleosporoides; genotype TDa 95-310

text: Dioscorea alata

text: leaf

characteristic: title


text: Northwest A&F University

text: live

text: Generic

text: Analyze the differentially expressed genes in ovary of dairy goat using suppressive subtractive hybridization

text: day 10 of estrus cycle

text: Capra hircus

text: Female

text: Xinong Saanen dairy goat

characteristic: strain

text: Ovary

text: Goat ovary in days 10 of estrus cycle Library


text: College of Life Science, Wuhan University

text: live

text: Generic

text: zygote

characteristic: cell type

characteristic: cultivar

text: zygote cells were manually collected from ovules. RNA was isolated and cDNA library was constructed by using the Clontech SMART cDNA library construction kit. The cDNAs were directionally ligated into pTripEX2.

text: after fertilization

characteristic: lab-host

text: Nicotiana tabacum

text: Sfi IA

text: Sfi IB

text: Tobacco zygote cell cDNA library

text: Phage


characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: female gamete

text: Egg cells were manually collected from ovules. RNA was isolated and cDNA library was constructed by using the Clontech SMART cDNA library construction kit. The cDNAs were directionally ligated into pTripEX2.

text: Before fertilization

text: Nicotiana tabacum

text: Sfi IA

text: Sfi IB

text: Tobacco egg cell cDNA library

text: Phage


text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: two-celled proembryo

text: Two-celled proembryo were manually collected from ovules. RNA was isolated and cDNA library was constructed by using the Clontech SMART cDNA library construction kit. The cDNAs were directionally ligated into pTripEX2.

text: after first zygote division

text: Nicotiana tabacum

text: Sfi IA

text: Sfi IB

text: Tobacco two-celled proembryo cDNA library

text: Phage