text: Clemson University

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: Morex

characteristic: cultivar

text: Seeds were surface sterilized then germinated under axenic conditions in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Five-day old seedling shoots were then harvested, total RNA was prepared, poly(A) RNA was purified, one primary unamplified cDNA library was made, and 1 million pfu were in vivo excised to give pBluescript SK(-) cDNA phagemids. These steps were performed in the TJ Close laboratory at the University of California, Riverside (Choi, Close, Fenton). Phagemids were plated and picked at the Clemson University Genomics Institute (CUGI) (Begum, Palmer, Frisch, Atkins and Wing). Plasmid DNA preparations, DNA sequencing and sequence analysis were performed at CUGI (Wing, Yu, Frisch, Henry, Simmons, Oates, Rambo, Main). The sequence has been trimmed to remove vector sequence and contains a minimum of 100 bases of phred value 20 or above. For more details on library preparation and sequence analysis see http://www.genome.clemson.edu/projects/barley. To order this clone see http://www.genome.clemson.edu/orders Also see Close TJ, Wing R, Kleinhofs A, Wise R (2001) Genetically and physically anchored EST resources for barley genomics. Barley Genetics Newsletter 31:29-30. (http://wheat.pw.usda.gov/ggpages/bgn/31/cover.html)

text: Hordeum vulgare subsp. vulgare

text: Seedling shoot

text: Hordeum vulgare seedling shoot EST library HVcDNA0003 (Etiolated and unstressed)

text: Phage

text: lambdaZAP


status: PUBLIC

characteristic: External Id

text: Clemson University

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Morex

tag: attribute

text: Seeds were surface sterilized then germinated under axenic conditions in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Five-day old seedlings were incubated at 5oC for 2 days. Shoots were then harvested, total RNA was prepared, poly(A) RNA was purified, one primary unamplified cDNA library was made, and 600000 pfu were in vivo excised to give pBluescript SK(-) cDNA phagemids. These steps were performed in the TJ Close laboratory at the University of California, Riverside (Choi, Close, Fenton). Phagemids were plated and picked at the Clemson University Genomics Institute (CUGI) (Begum, Palmer, Frisch, Atkins and Wing). Plasmid DNA preparations, DNA sequencing and sequence analysis were performed at CUGI (Wing, Yu, Frisch, Henry, Simmons, Oates, Rambo, Main). The sequence has been trimmed to remove vector sequence and contains a minimum of 100 bases of phred value 20 or above. For more details on library preparation and sequence analysis see http://www.genome.clemson.edu/projects/barley. To order this clone see http://www.genome.clemson.edu/orders Also see Close TJ, Wing R, Kleinhofs A, Wise R (2001) Genetically and physically anchored EST resources for barley genomics. Barley Genetics Newsletter 31:29-30. (http://wheat.pw.usda.gov/ggpages/bgn/31/cover.html)

characteristic: description

characteristic: lab-host

text: Hordeum vulgare subsp. vulgare

characteristic: organism

text: Seedling shoot

characteristic: tissue-type

text: Hordeum vulgare seedling shoot EST library HVcDNA0001 (Cold stress)

characteristic: title

text: Phage

text: lambdaZAP

characteristic: vector


text: Clemson University

text: live

text: Generic

text: Morex

text: Seeds were surface sterilized then germinated under axenic conditions in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Five-day old seedlings were incubated at 90% RH for 24 hr. Shoots were then harvested, total RNA was prepared, poly(A) RNA was purified, one primary unamplified cDNA library was made, 600000 pfu were in vivo excised to give pBluescript SK(-) cDNA phagemids. These steps were performed in the TJ Close laboratory at the University of California, Riverside (Choi, Close, Fenton). Phagemids were plated and picked at the Clemson University Genomics Institute (CUGI) (Begum, Palmer, Frisch, Atkins and Wing). Plasmid DNA preparations, DNA sequencing and sequence analysis were performed at CUGI (Wing, Yu, Frisch, Henry, Simmons, Oates, Rambo, Main). The sequence has been trimmed to remove vector sequence and contains a minimum of 100 bases of phred value 20 or above. For more details on library preparation and sequence analysis see http://www.genome.clemson.edu/projects/barley. To order this clone see http://www.genome.clemson.edu/orders Also see Close TJ, Wing R, Kleinhofs A, Wise R (2001) Genetically and physically anchored EST resources for barley genomics. Barley Genetics Newsletter 31:29-30. (http://wheat.pw.usda.gov/ggpages/bgn/31/cover.html)

text: Hordeum vulgare subsp. vulgare

text: Seedling shoot

text: Hordeum vulgare seedling shoot EST library HVcDNA0002 (Dehydration stress)

text: Phage

text: lambdaZAP


text: University of Leeds

text: live

text: Generic

text: CD4+ T-cell clone

characteristic: cell type

characteristic: cell-line

text: Homo sapiens

text: Homo sapiens CD4+ T-cell clone HA1.7


text: The Samuel Roberts Noble Foundation

text: live

text: Generic

text: Cells were induced with yeast cell wall extracts equivalent to 50ug/ml glucose in the final concentration. Samples were taken at 0.5, 1, 12 and 24 hours after induction. Equal amounts of RNA from each time point were pooled and used for mRNA isolation.

text: Cell suspensions were subcultured every 14 days. Cells were induced six days after subculture

characteristic: dev-stage

text: Medicago truncatula

text: Cell cultures derived from root tissues

text: Elicited cell culture

text: Plasmid

text: Lambda Zap


text: University of California, Los Angeles, School of Medicine

characteristic: INSDC center name

text: live

text: Generic

text: bFGF Cultured Neurospheres

text: Cells were harvested from P0 mouse cortex and cultured for 4 weeks in the presence of bFGF to grow neurospheres. Half of the cells were then placed under differentiating conditions for 24 hours. RNA was extracted from both groups using acid phenol extraction, PolyA(+) RNA was purified using oligo dt paramagnetic beads, and cDNA was synthesized. Amplicons were created from Dpn II digested cDNA and 2 rounds of RDA subtraction were performed using amplicons from the differentiated cells as the driver. Resultant library, enriched in clones from the undifferentiated neural progenitors, was then shotgun cloned into BamH1 digested Bluescript SK II+ and transformed into E. coli.

text: Hbl electromax DH10B (GibcoBrl)

text: Brain

characteristic: organ

text: Mus musculus

text: BamHI

characteristic: strain

text: Cerebral Cortex

text: neural progenitor enriched library

text: Plasmid

text: pBluescript KS II+


characteristic: External Id

text: University of Edinburgh

characteristic: INSDC first public

text: live

text: Generic

text: Echinococus granulosus is a cestode parasite of dogs (definitive host) and various domestic and wild animals as well as humans (intermediate hosts). The library was constructed from protoscoleces obtained from one hydatid cyst present in the lungs of a naturally infected Uruguayan bovine. Parasite cDNA synthesis was primed with a NotI primer-adapter cDNA was ligated to SalI adapters, digested with NotI, and cloned into pSPORT 1.

text: larva (protoscolex)

text: Echinococcus granulosus

text: SalI

text: NotI

text: hermaphrodite

characteristic: sex

text: Echinococcus granulosus (parasitic cestode) protoscolex


text: The University of Georgia, Department of Plant Biology

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: The library was made from poly-A RNA in the cloning vector lambda ZAP II. Clones to be sequenced were prepared by mass excision.

text: Mix of ovaries of varying immature stages from 8-week-old plants

text: Sorghum bicolor

text: XhoI

text: EcoRI

text: Ovary 2 (OV2)

text: Plasmid

text: pBluescript II from Lambda Zap II


text: University of Wolverhampton

text: live

text: Generic

characteristic: NCBI submission package

text: Allium sativum

text: Shoot

text: Allium sativum shoot

text: Plasmid

text: pGEM-T


text: Lawrence Livermore National Laboratory

text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.75 kb. Constructed by Life Technologies. Note: this is a NCI_CGAP Library. |

characteristic: description

text: DH10B (T1 phage-resistant)

characteristic: lab-host

text: kidney

text: Mus musculus

characteristic: organism

text: NotI

text: SalI

text: FVB/N

text: phagemid (ampicillin resistant)


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: cDNA made by oligo-dT priming. Directionally cloned into EcoRI/XhoI sites using the following 5' adaptor: GGCACGAG(G). Library constructed by Ling Hong in the laboratory of Gerald M. Rubin (University of California, Berkeley) using ZAP-cDNA synthesis kit (Stratagene) and Superscript II RT (Life Technologies). Note: this is a NIH_MGC Library. |

text: DH10B (phage-resistant)

text: eye

text: Homo sapiens

text: XhoI

text: EcoRI

text: normal pigmented retinal epithelium

characteristic: tissue-type

characteristic: title

text: plasmid (chloramphenicol resistant, grown at 30C)

characteristic: vector


text: live

text: Generic

text: cDNA made by oligo-dT priming. Directionally cloned into EcoRI/XhoI sites using the following 5' adaptor: GGCACGAG(G). Size-selected >500bp for average insert size 1.8kb. Library constructed by Ling Hong in the laboratory of Gerald M. Rubin (University of California, Berkeley) using ZAP-cDNA synthesis kit (Stratagene) and Superscript II RT (Life Technologies). Note: this is a NIH_MGC Library. |

text: DH10B (phage-resistant)

text: skin

text: Homo sapiens

text: XhoI

text: EcoRI

text: melanotic melanoma, high MDR (cell line)


text: Iowa State University

text: live

text: Generic

text: The MI-P-A1 library is derived from anterior pituitary at estrus day 0. For a detailed description of the library from which this clone was derived, please visit our web site at http://pigest.genome.iastate.edu/.

text: DH10B (Life Technologies)

text: Sus scrofa

text: Not I

text: EcoRI

text: crossbreed


text: Iowa State University

text: live

text: Generic

text: The MI-P-A2 library is derived from anterior pituitary at estrus day 5. For a detailed description of the library from which this clone was derived, please visit our web site at http://pigest.genome.iastate.edu/.

text: DH10B (Life Technologies)

text: Sus scrofa

text: Not I

text: EcoRI

text: crossbreed


text: Iowa State University

text: live

text: Generic

text: The MI-P-A3 library is derived from anterior pituitary at estrus day 12. For a detailed description of the library from which this clone was derived, please visit our web site at http://pigest.genome.iastate.edu/.

text: DH10B (Life Technologies)

text: Sus scrofa

text: Not I

text: EcoRI

text: crossbreed


text: Iowa State University

text: live

text: Generic

text: The MI-P-E2 library is derived from fetus at gestational day 20. For a detailed description of the library from which this clone was derived, please visit our web site at http://pigest.genome.iastate.edu/.

text: DH10B (Life Technologies)

text: Sus scrofa

text: Not I

text: EcoRI

text: crossbreed


text: Iowa State University

text: live

text: Generic

text: The MI-P-E3 library is derived from fetus at gestational day 45. For a detailed description of the library from which this clone was derived, please visit our web site at http://pigest.genome.iastate.edu/.

text: DH10B (Life Technologies)

text: Sus scrofa

text: Not I

text: EcoRI

text: crossbreed


text: Iowa State University

text: live

text: Generic

text: The MI-P-E4 library is derived from embryo at gestational day 14. For a detailed description of the library from which this clone was derived, please visit our web site at http://pigest.genome.iastate.edu/.

text: DH10B (Life Technologies)

text: Sus scrofa

text: Not I

text: EcoRI

text: crossbreed


text: Iowa State University

text: live

text: Generic

text: The MI-P-E5 library is derived from embryo at gestational day 12. For a detailed description of the library from which this clone was derived, please visit our web site at http://pigest.genome.iastate.edu/.

text: DH10B (Life Technologies)

text: Sus scrofa

text: Not I

text: EcoRI

text: crossbreed


text: Iowa State University

text: live

text: Generic

text: The MI-P-E6 library is derived from fetus at gestational day 20. For a detailed description of the library from which this clone was derived, please visit our web site at http://pigest.genome.iastate.edu/.

text: DH10B (Life Technologies)

text: Sus scrofa

text: Not I

text: EcoRI

text: crossbreed


text: Iowa State University

text: live

text: Generic

text: The MI-P-H1 library is derived from hypothalamus at estrus day 0. For a detailed description of the library from which this clone was derived, please visit our web site at http://pigest.genome.iastate.edu/.

text: DH10B (Life Technologies)

text: Sus scrofa

text: Not I

text: EcoRI

text: crossbreed


text: Iowa State University

text: live

text: Generic

text: The MI-P-H2 library is derived from hypothalamus at estrus day 5. For a detailed description of the library from which this clone was derived, please visit our web site at http://pigest.genome.iastate.edu/.

text: DH10B (Life Technologies)

text: Sus scrofa

text: Not I

text: EcoRI

text: crossbreed


text: Iowa State University

text: live

text: Generic

text: The MI-P-H3 library is derived from hypothalamus at estrus day 12. For a detailed description of the library from which this clone was derived, please visit our web site at http://pigest.genome.iastate.edu/.

text: DH10B (Life Technologies)

text: Sus scrofa

text: Not I

text: EcoRI

text: crossbreed


text: Iowa State University

text: live

text: Generic

text: The MI-P-H4 library is derived from hypothalamus at estrus day 5. For a detailed description of the library from which this clone was derived, please visit our web site at http://pigest.genome.iastate.edu/.

text: DH10B (Life Technologies)

text: Sus scrofa

text: Not I

text: EcoRI

text: crossbreed


text: Iowa State University

text: live

text: Generic

text: The MI-P-O1 library is derived from ovary at estrus day 0. For a detailed description of the library from which this clone was derived, please visit our web site at http://pigest.genome.iastate.edu/.

text: DH10B (Life Technologies)

text: Sus scrofa

text: Not I

text: EcoRI

text: crossbreed


text: Iowa State University

text: live

text: Generic

text: The MI-P-O2 library is derived from ovary at estrus day 5. For a detailed description of the library from which this clone was derived, please visit our web site at http://pigest.genome.iastate.edu/.

text: DH10B (Life Technologies)

text: Sus scrofa

text: Not I

text: EcoRI

text: crossbreed


text: Iowa State University

text: live

text: Generic

text: The MI-P-O3 library is derived from ovary at estrus day 12. For a detailed description of the library from which this clone was derived, please visit our web site at http://pigest.genome.iastate.edu/.

text: DH10B (Life Technologies)

text: Sus scrofa

text: Not I

text: EcoRI

text: crossbreed


text: Iowa State University

text: live

text: Generic

text: The MI-P-AY0 library is derived from placenta. For a detailed description of the library from which this clone was derived, please visit our web site at http://pigest.genome.iastate.edu/. The procedure used to create this library has been previously described (Bonaldo, Lennon and Soares, Genome Research 6:791-806, 1996)

text: DH10B (Life Technologies)

text: Sus scrofa

text: Not I

text: EcoRI


text: Iowa State University

text: live

text: Generic

text: The MI-P-AY1 library is normalized library derived from the MI-P-AY0 library, ultimately derived from placenta tissue. For a detailed description of the library from which this clone was derived, please visit our web site at http://pigest.genome.iastate.edu/. The procedure used to create this library has been previously described (Bonaldo, Lennon and Soares, Genome Research 6: 791-806, 1996)

text: DH10B (Life Technologies)

text: Sus scrofa

text: Not I

text: EcoRI

text: crossbreed


text: Tianjin University

text: live

text: Generic

text: Taxus cuspidata Sieb. et Zucc; Plant suspension culture

text: Taxus cuspidata

text: Taxus cuspidata cDNA


text: University of Wolverhampton

text: live

text: Generic

text: Maythorpe and Golden Promise

characteristic: cultivar

text: Isolated by mRNA differential display, the plants were treated with 150 mM NaCl

text: Hordeum vulgare subsp. vulgare

text: Shoot

text: Hordeum vulgare treated with 150 mM NaCl

text: Plasmid

text: pGEM-T Easy


text: University of Glasgow, Bearsden Road, Glasgow, G20 8RP, UK

characteristic: INSDC center name

text: live

text: Generic

text: Bovine transcripts were identified by differential mRNA display from Theileria annulata infected bovine clonal cell lines. Transcripts were differentially expressed between the differentiating D7 clonal cell line and the non-differentiating D7B12 clonal cell line.

text: Bos taurus

text: Bos taurus from Theileria annulata infected bovine clonal cell line


text: live

text: Generic

text: Theileria annulata transcripts were identified by differential mRNA display from Theileria annulata infected bovine clonal cell lines.

text: Theileria annulata

text: Theileria annulata from Theileria annulata infected bovine clonal cell line


text: The University of Georgia, Department of Plant Biology

text: live

text: Generic

text: The library was made from poly-A RNA in the cloning vector lambda ZAP II. Clones to be sequenced were prepared by mass excision.

text: Rhizomes

characteristic: organ

text: Sorghum propinquum

text: XhoI

text: EcoRI

text: Rhizome2 (RHIZ2)

text: Plasmid

text: pBluescript II from Lambda Zap II


text: live

text: Generic

text: Anchor enzyme: NlaIII~ Library constructed in the laboratory of N. Papadopolous (Columbia University).

text: prostate

text: Homo sapiens

text: male

text: bulk tumor, primary

text: plasmid


text: live

text: Generic

text: prostate

text: Homo sapiens

text: male

text: normal bulk tissue

text: plasmid


text: live

text: Generic

text: prostate

text: Homo sapiens

text: male

text: advanced tumor

text: plasmid


text: CuraGen Corporation

text: live

text: Generic

text: BMS

characteristic: cell-line

text: Zea mays

characteristic: strain

text: Zea mays BMS


characteristic: External Id

text: National Research Institute for Family Planning, Beijing

characteristic: INSDC first public

text: live

text: Generic

text: Rattus norvegicus

text: testis

text: Rattus norvegicus testis (M. Jia)


text: University of Florida

characteristic: INSDC last update

text: live

text: Generic

text: Metacerebral cell

characteristic: cell type

text: Aplysia californica

text: nervous system

text: Aplysia metacerebral cell cDNA library

text: Plasmid

text: pGEM-T


text: University of Bonn

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: cDNA fragments obtained from differential display RT-PCR banding patterns were cloned into pGEM

text: liver

text: Sus scrofa

text: differential display RT-PCR clones


text: Adelaide University

text: live

text: Generic

characteristic: NCBI submission package

text: 6-9 hours post fertilisation

characteristic: dev-stage

text: Danio rerio

text: zebrafish 6-9 hpf embryonic cDNA library

text: phagemid

text: pBluescript II KS (+/-)


text: Washington University School of Medicine

text: live

text: Generic

text: The cDNA library was constructed from mRNA isolated from hypocotyl tissue of 2 week old etiolated seedlings for PI468916. Complementary DNA was synthesized from mRNA using a primer consisting of a poly(dT) sequence with a XhoI restriction site. EcoRI adapters were ligated to the blunt-ended cDNA fragments followed by XhoI digestion. The cDNA fragments were directionally cloned into the EcoRI-XhoI restriction site of the pBluescript vector. The ligated cDNA fragments were transformed into DH10B host cells (Gibco BRL). This library was constructed in the laboratory of Dr. Randy Shoemaker at Iowa state university.

characteristic: description

characteristic: lab-host

text: Glycine soja

characteristic: organism

text: EcoRI

text: XhoI

text: Hypocotyl, 2 week old seedlings, etiolated

text: plasmid

text: pBluescript II SK+


text: USDA-ARS, Appalachian Fruit Research Station

text: live

text: Generic

text: Loring

text: Fruit cDNA. The mRNA was isolated from whole fruit from which the stone was excised. The fruit was near ripe, registering from 21 to 60 newtons resistance to a penetrometer. The library was constructed using the lambda UniZap XR by Stratagene.

text: 21-60 Newtons flesh resistance

text: Prunus persica

text: EcoRI

text: XhoI

text: Whole fruit minus the stone

characteristic: tissue-type

text: near-ripe peach fruit cDNA library

characteristic: title

text: Phage

text: Lambda vector UniZap XR

characteristic: vector


text: National Cancer Institute

characteristic: INSDC center name

text: live

text: Generic

text: keratinocyte

text: primary

text: Total RNA was extracted, reverse transcribed, and directionally cloned using uracyl DNA glycosylase (Krizman protocol 2, see http:/www.ncbi.nlm.nih.gov/ CGAP/info/libconst.cgi#Protocol2).

text: newborn

text: foreskin

characteristic: organ

text: Homo sapiens

text: male

characteristic: sex

text: epidermis

text: normal human keratinocytes

text: plasmid


characteristic: External Id

text: National Cancer Institute

text: live

text: Generic

text: Epidermis from 20 cryostat skin sections (8 um thick) was selectively isolated using laser capture microdissection. Total RNA was extracted, reverse transcribed, and directionally cloned using uracyl DNA glycosylase (Krizman protocol 2, see http:/www.ncbi.nlm.nih.gov/ CGAP/info/libconst.cgi#Protocol2).

text: adult

text: skin

text: Homo sapiens

text: Male

text: epidermis

text: microdissected normal human epidermis

text: plasmid


text: Sun Yat-sen University of Medical Sciences

characteristic: INSDC first public

text: live

text: Generic

text: The mRNA were extracted from Nasopharyngeal Carcinoma and Nasopharyngeal tissues respectively. Using suppression subtractive hybridization technique the subtractive cDNA pools were constructed. The cDNA pools were inserted into pT-Adv vector then transfected to E.coli to get the subtractive cDNA library.

text: Homo sapiens

text: Nasopharyngeal Tissue

text: Human subtractive cDNA library


text: John Innes Centre

characteristic: INSDC last update

text: live

text: Generic

text: Moneymaker Cf-9

characteristic: cultivar

text: infiltrated with the Avr9 peptide from the fungus Cladosporium fulvum

text: 14-16 day old seedlings

text: Solanum lycopersicum

text: cotyledon

text: Lycopersicon esculentum cotyledon 14-16 day old seedlings


text: Korea University

text: live

characteristic: INSDC status

text: Generic

text: Homo sapiens

text: Homo sapiens library (Park SH)


text: University of Bologna

text: live

text: Generic

characteristic: NCBI submission model

text: line N28

text: Transcripts identified by use of cDNA-AFLP

text: Zea mays

text: meristems

text: Maize meristems cDNA-AFLP selected clones

text: Plasmid

text: pGEM-T Easy


text: University of Bologna

text: live

text: Generic

characteristic: NCBI submission package

text: line N28

text: Zea mays

text: leaves

text: Maize leaves cDNA-AFLP selected clones

text: Plasmid

text: pGEM-T Easy


text: Seoul National University

text: live

text: Generic

text: Habanero

text: Performed cDNA subtraction between pungent (Capsicum chinense cv. Habanero) and non-pungent pepper (Capsicum annuum cv. Haehwa 3)

text: 30 days after flowering

characteristic: dev-stage

text: E.coli DH10B

text: Capsicum chinense

text: Placenta

text: cDNA subtraction library of pungent pepper

text: Plasmid

text: pGEM-T Easy vector


text: National Institute of Agricultural Science & Technology

text: live

text: Generic

text: average insert size:1000 bp; initial pfu:1.200 x 10 6

characteristic: description

text: XL1-Blue MRF', SOLR

text: Bombyx mori

text: Bombyx mori Bm5 Cell Tunicamycin Induced cDNA Library

text: Uni-ZAP XR


text: Institute for Plant Genetics and Crop Plant Research

text: live

text: Generic

text: Barke

text: mRNA was made from roots of spring barley variety 'Barke', a high quality malting variety. Roots were grown for two days on filter paper at room temperature Cloning sites: EcoRI (5'-end of cDNA) and XhoI (3'-end of cDNA). NOTE: Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRI adapter. Average insert size is 1 kb Sequence trimming: Vector sequences and sequence ends were trimmed from the 5'-and 3'-end until a 50 bp window contains less than two ambiguities. The maximum length was set to 700 bp

text: XLOLR

characteristic: lab-host

text: Hordeum vulgare subsp. vulgare

text: EcoRI

text: XhoI

text: roots

text: Hordeum vulgare Barke roots

text: plasmid pBK-CMV


text: live

text: Generic

text: Barke

text: mRNA was made from developing caryopsis (3.-15.DAP) of spring barley variety 'Barke', a high quality malting variety. Cloning sites: EcoRI (5'-end of cDNA) and XhoI (3'-end of cDNA). NOTE: Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRI adapter. Average insert size is 1 kb Sequence trimming: Vector sequences and sequence ends were trimmed from the 5'-and 3'-end until a 50 bp window contains less than two ambiguities. The maximum length was set to 700 bp

text: XLOLR

text: Hordeum vulgare subsp. vulgare

characteristic: organism

text: EcoRI

text: XhoI

text: developing caryopsis (3.-15.DAP)

text: Hordeum vulgare Barke developing caryopsis (3.-15.DAP)

text: plasmid pBK-CMV


text: National Institute for Medical Research

text: live

text: Generic

text: Chick embryonic hindbrain cDNA directionally cloned into pSPORT1/NotI/SalI using the Superscript Plasmid System for cDNA Synthesis and Plasmid Cloning (Life Technologies)

text: Hamburger Hamilton stage 10-12

text: Embryonic hindbrain

text: Gallus gallus

text: SalI

text: NotI

text: Chick embryonic hindbrain cDNA library

text: Plasmid


text: National Eye Institute

text: live

text: Generic

text: Two human lenses from different adults (both approximately 40 years old) together yielded 20ug of total RNA and 150ng mRNA for cDNA library synthesis. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies, essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt end inserts were cloned into the Not I/EcoR V sites in the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC).

text: Adult

text: Eye

text: Homo sapiens

text: Lens

characteristic: tissue-type

text: Human Lens cDNA (Un-normalized, unamplified): BY

text: Plasmid


text: National Eye Institute

text: live

text: Generic

text: Post-mortem iris tissue was pooled from 10 individuals ranging in age from 4-80 years and RNA was extracted. From this pooled sample an aliquot of 60ug of total RNA yielded 2.17ug of mRNA. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies, essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt end inserts were cloned into the Not I/EcoR V sites in the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC).

text: Adult

text: Eye

text: Homo sapiens

text: Iris

text: Human Iris cDNA (Un-normalized, unamplified): BX

characteristic: title

text: Plasmid


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: Cloned unidirectionally; oligo-dT primed. Average insert size 1.229 kb. Library enriched for full-length clones and constructed by Life Technologies. Note: this is a NIH_MGC Library.

text: DH10B (phage-resistant)

text: adrenal gland

text: Homo sapiens

text: NotI

text: SalI

text: adrenal cortex carcinoma, cell line

text: phagemid (ampicillin resistant)

characteristic: vector


characteristic: INSDC center name

text: live

text: Generic

text: Cloned unidirectionally; oligo-dT primed. Average insert size 1.867 kb. Library enriched for full-length clones and constructed by Life Technologies. Note: this is a NIH_MGC Library.

text: DH10B (phage-resistant)

text: lymph

text: Homo sapiens

text: NotI

text: SalI

text: lymphoma, cell line

text: phagemid (ampicillin resistant)


text: Stanford University

text: live

text: Generic

text: This library represents the unique ESTs found in the first round of EST sequencing at Stanford University for the maize genome project. Sequences are present from libraries 486, 487, 496, 603, 605, 606, 614, 618, 660, 683, 687, 707, and 945. Contigs were assembled using TIGR's CAP program and a representative EST from each contig was selected for the Unigene set. All singlets were also selected.

text: Zea mays

text: 1000 - Unigene I from Maize Genome Project


text: Universite Libre de Bruxelles

text: live

text: Generic

text: salivary cells

characteristic: cell type

text: adult

text: Ixodes ricinus

text: female

text: salivary glands

text: Ixodes ricinus salivary glands adult


text: live

text: Generic

text: mRNA made from myeloid progenitors, cDNA made by oligo-dT priming. Directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 300 bp. Primary library. cDNA Library Preparation: David B. Krizman, Ph.D.

text: blood

characteristic: organ

text: Mus musculus

text: flow-sorted, common myeloid progenitors

text: plasmid (ampicillin resistant)


characteristic: External Id

text: live

text: Generic

text: mRNA made from granulocyte-macrophage progenitors, cDNA made by oligo-dT priming. Directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 300 bp. Primary library. cDNA Library Preparation: David B. Krizman, Ph.D.

text: blood

text: Mus musculus

text: flow-sorted, common granulocyte-macrophage progenitors

text: plasmid (ampicillin resistant)


characteristic: INSDC first public

text: live

text: Generic

text: mRNA made from flow-sorted lymphocytes, cDNA made by oligo-dT priming. Directionally cloned. Average insert size 1.8 kb. Primary library, non-amplified. cDNA Library Preparation: David B. Krizman, Ph.D.

text: DH10B (T1-resistant)

text: lymphocytes (flow-sorted)

text: Mus musculus

text: marginal zone B-cell tumor

text: plasmid (ampicillin resistant)

text: pCMV-SPORT6 (Life Technologies)


characteristic: INSDC last update

text: live

text: Generic

text: mRNA made from flow-sorted NK cells, cDNA made by oligo-dT priming. Directionally cloned. Average insert size 1.5 kb. Primary library, non-amplified. cDNA Library Preparation: David B. Krizman, Ph.D.

text: DH10B (T1-resistant)

text: spleen

text: Mus musculus

text: NK cells (flow-sorted)

text: plasmid (ampicillin resistant)

text: pCMV-SPORT6 (Life Technologies)


text: Heska Corporation

text: live

text: Generic

text: The library was made using the PCR-Select( cDNA Subtraction kit (CLONTECH Laboratories, Inc.) with 2 (g hindgut and Malpighian tubule mRNA used as to make the 'tester' cDNA and 2 (g carcass (all flea tissues minus the hindgut and Malpighian tubules) to make the 'driver' cDNA. Suppression PCR and subtractive hybridization techniques are then used to enrich for cDNAs in the tester pool that are not also present in the driver pool.

text: unfed adult and 24 hour cat blood-fed adults (1:1 ratio)

text: Ctenocephalides felis

text: female and male (4:1 ratio)

characteristic: sex

text: hindgut and Malpighian tubule

text: hindgut and Malpighian tubule subtracted cDNA library


text: University of Chicago Medical Center

text: live

characteristic: INSDC status

text: Generic

text: GR-1+ myeloid progenitor cells

text: Mus musculus

text: female

characteristic: strain

text: bone marrow

text: mouse GR-1+ myeloid progenitor cells cDNA Library

text: Plasmid


text: Institute of Digestive Disease

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Liver

text: Homo sapiens

text: Human hepatocellular carcinoma subtracted cDNA library


text: Department of Zoology, National Chung Hsing University

text: live

text: Generic

text: 4-cell; embryonic day 2.5 post-fertilization

characteristic: dev-stage

text: embryo

text: Mus musculus

text: ICR

text: embryo

text: Mouse four-cell stage embryo cDNA


text: live

text: Generic

text: Unfertilized egg; Day 0.5 after ovulation

text: Oocyte

text: Mus musculus

text: ICR

text: Oocyte

text: Mouse unfertilized-egg cDNA


text: Ludwig Institute for Cancer Research

text: live

text: Generic

text: A mini-library was made by cloning products derived from ORESTES PCR (U.S. Letters Patent application No. 196,716 - Ludwig Institute for Cancer Research) profiles into the pUC 18 vector. Reverse transcription of tissue mRNA and cDNA amplification were performed under low stringency conditions.

characteristic: description

text: Adult

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: Ludwig Institute for Cancer Research

text: live

text: Generic

text: Adult

text: breast

text: Homo sapiens

characteristic: organism

text: SmaI

text: SmaI

text: Plasmid


text: Ludwig Institute for Cancer Research

text: live

text: Generic

text: Adult

text: colon

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: Ludwig Institute for Cancer Research

text: live

text: Generic

text: Adult

text: marrow

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: Ludwig Institute for Cancer Research

text: live

text: Generic

text: Adult

text: ovary

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: Heska Corporation

text: live

text: Generic

text: This is a standard lambda phage cDNA library

text: Ctenocephalides felis

text: female and male (4:1 ratio)

text: hindgut and Malpighian tubule

characteristic: tissue-type

text: hindgut and Malpighian tubule cDNA library


text: York University

text: live

text: Generic

text: Vegetative

text: Tetrahymena thermophila

text: Tetrahymena 1

characteristic: title

text: pCRII


text: York University

text: live

text: Generic

text: Vegetative

text: Tetrahymena thermophila

text: Tetrahymena 2

text: pBluescript

characteristic: vector


text: China Agriculture University

characteristic: INSDC center name

text: live

text: Generic

characteristic: lab-host

text: Gallus gallus

text: EcoRI

text: hypothalamus

text: CHH

text: lambda gt10


text: University of Insubria

text: live

text: Generic

text: Xenopus laevis

text: Whole 5-days embryos

text: cDNA from differential display on Platinum-treated embryos


text: University of Texas Health Science Center at San Antonio

text: live

text: Generic

text: 36 hour spherules

text: Coccidioides posadasii

text: Silveira

text: Coccidioides immitis 36 hour spherules


text: live

text: Generic

characteristic: cell-line

text: Anchor enzyme: NlaIII~ Glioblastoma multiforme cell line H566 has been verified to be telomerase positive via TRAP assay, RT-PCR, and quantitative FRET RT-PCR. The cells were grown in zinc option media with 5% FBS. Library constructed using the MicroSAGE adaption (Science 270:484-487) in the laboratory of G. Riggins, M.D., Ph.D. (Duke University).

text: brain

characteristic: organ

text: Homo sapiens

text: glioblastoma multiforme

text: plasmid


characteristic: External Id

text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

characteristic: INSDC first public

text: live

text: Generic

text: Cloned unidirectionally; oligo-dT primed. Average insert size 1.533 kb. Library enriched for full-length clones and constructed by Life Technologies. Note: this is a NIH_MGC Library.

text: DH10B (phage-resistant)

text: bone

text: Homo sapiens

text: NotI

text: SalI

text: osteosarcoma, cell line

text: phagemid (ampicillin resistant)


characteristic: INSDC last update

text: live

text: Generic

text: Oligo-dT primed using primer 5'-TTTTTTTTTTTTTTTTVN-3', size-selected for average insert size 2.5 kb and normalized to ROT 5. This is a primary library enriched for full-length clones and constructed using the Cap-trapper method (Carninci, in preparation). Library constructed by M. Brownstein (NIMH/NHGRI, National Institutes of Health). Note: this is a NIH_MGC Library.

text: brain

text: Homo sapiens

text: BamHI

text: SalI-XhoI (gtcgag)

text: hippocampus

text: phagemid (ampicillin resistant)

text: pBluescriptR (modified pBluescript KS+)


text: live

characteristic: INSDC status

text: Generic

text: Oligo-dT primed using primer 5'-TTTTTTTTTTTTTTTTVN-3', size-selected for average insert size 2.3 kb and normalized to ROT 5. This is a primary library enriched for full-length clones and constructed using the Cap-trapper method (Carninci, in preparation). Library constructed by M. Brownstein (NIMH/NHGRI, National Institutes of Health). Note: this is a NIH_MGC Library.

text: brain

text: Homo sapiens

text: BamHI

text: SalI-XhoI (gtcgag)

text: hypothalamus

text: phagemid (ampicillin resistant)

text: pBluescriptR (modified pBluescript KS+)


text: live

text: Generic

text: Oligo-dT primed using primer 5'-TTTTTTTTTTTTTTTTVN-3', size-selected for average insert size 2.2 kb and normalized to ROT 5. This is a primary library enriched for full-length clones and constructed using the Cap-trapper method (Carninci, in preparation). Library constructed by M. Brownstein (NIMH/NHGRI, National Institutes of Health). Note: this is a NIH_MGC Library.

text: testis

text: Homo sapiens

text: BamHI

text: SalI-XhoI (gtcgag)

text: phagemid (ampicillin resistant)

text: pBluescriptR (modified pBluescript KS+)


text: JST (Japan Science and Technology Corporation)

text: live

text: Generic

text: This Subtraction cDNA library from postnatal day 7 mouse brain cortex containing differencially expressed genes which deprived all facial whiskers for P0-P7.

text: Postnatal day 7

text: brain

text: Mus musculus

characteristic: strain

text: cortex

text: Postnatal day 7 mouse brain cortex cDNA library

text: Plasmid

text: pT-Adv (CLONTECH)


text: Chinese PLA General Hospital

text: live

text: Generic

characteristic: NCBI submission model

text: Homo sapiens

text: paratumor gastric mucosa

text: Homo sapiens paratumor gastric mucosa


text: Duke University

text: live

text: Generic

characteristic: NCBI submission package

text: This library, constructed by John Davies and Jeffrey McDermott, combines cDNAs from CC-1690 cells grown to mid-log phase in TAP-N (30 min, 1hr, 4hr), TAP-S (30 min, 1hr, 4hr), TAP-P (4hr, 12hr, 24hr), NO3 to NH4 (30min, 1hr, 4hr) and NH4 to NO3 (30min, 1hr, 4hr). PolyA mRNA was purified from each sample, pooled and cDNA synthesized. The cDNA was directionally cloned into lambda Zap II (Stratagene) in the EcoRI (5') and XhoRI (3') sites. pBluescript II SK- plasmids were excised from the lambda ZAP clones by superinfection with ExAssist (Stratagene) phage. The library was normalized using method 4 described in Bonaldo et al (1996) Genome Research 6: 791-806.

text: Chlamydomonas reinhardtii

text: EcoRI

text: XhoI

text: CC-1690 wild type mt+ 21gr

text: C. reinhardtii CC-1690, Stress condition I, normalized, Lambda Zap II

text: plasmid

text: pBluescript II SK-


text: Lawrence Livermore National Laboratory

text: live

text: Generic

text: mRNA made from thyroid carcinoma, cDNA made by oligo-dT priming. Non-directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 500 bp. Primary library. cDNA Library Preparation: David B. Krizman, Ph.D. REFERENCE: Krizman et al. (1996) Cancer Research 56:5380-5383.

text: thyroid

text: Homo sapiens

text: medullary carcinoma

text: plasmid (ampicillin resistant)


text: Southeast Missouri State University

text: live

text: Generic

text: 4-day-old mycelia of Schizophyllum commune were transferred from minimal (nitrogen-replete) medium to low-nitrogen medium. RNA was extracted six hours after transfer and cDNAs prepared.

text: Schizophyllum commune

text: EcoRI

text: XhoI

text: mycelium

text: 6HR Nitrogen-limited Schizophyllum library

text: Phage

text: lambda Zap


text: Southeast Missouri State University

text: live

text: Generic

text: 4-day-old mycelia of Schizophyllum commune were transferred from minimal (nitrogen-replete) medium to low-nitrogen medium. RNA was extracted twelve hours after transfer and cDNAs prepared.

text: Schizophyllum commune

text: EcoRI

text: XhoI

text: mycelium

text: M01 Nitrogen-limited Schizophyllum library

text: Phage

text: lambda Zap


text: Southeast Missouri State University

text: live

text: Generic

text: 4-day-old mycelia of Schizophyllum commune were transferred from minimal (nitrogen-replete) medium to fresh minimal medium. RNA was extracted twelve hours after transfer and cDNAs prepared.

text: Schizophyllum commune

text: EcoRI

text: XhoI

text: mycelium

text: MIN Nitrogen-replete Schizophyllum library

text: Phage

text: lambda Zap


text: University of Chicago Medical Center

text: live

text: Generic

text: CD15+ myeloid progenitor cells

characteristic: cell type

text: Homo sapiens

text: bone marrow

text: human CD15+ myeloid progenitor cells cDNA Library

text: Plasmid


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: Cloned unidirectionally; oligo-dT primed. Average insert size 1.383 kb. Library enriched for full-length clones and constructed by Life Technologies. Note: this is a NIH_MGC Library.

characteristic: description

text: DH10B (phage-resistant)

text: breast

text: Homo sapiens

text: NotI

text: SalI

text: mammary adenocarcinoma, cell line

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: Cloned unidirectionally; oligo-dT primed. Average insert size 1.767 kb. Library enriched for full-length clones and constructed by Life Technologies. Note: this is a NIH_MGC Library.

text: DH10B (phage-resistant)

text: small intestine

text: Homo sapiens

characteristic: organism

text: NotI

text: SalI

text: duodenal adenocarcinoma, cell line

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: Cloned unidirectionally; oligo-dT primed. Average insert size 1.7 kb. Library enriched for full-length clones and constructed by Life Technologies. Note: this is a NIH_MGC Library.

text: DH10B (phage-resistant)

text: liver

text: Homo sapiens

text: NotI

text: SalI

text: adenocarcinoma, cell line

characteristic: tissue-type

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: Cloned unidirectionally; oligo-dT primed. Average insert size 1.4 kb. Library enriched for full-length clones and constructed by Life Technologies. Note: this is a NIH_MGC Library.

text: DH10B (phage-resistant)

text: prostate

text: Homo sapiens

text: NotI

text: SalI

text: adenocarcinoma, cell line

characteristic: title

text: phagemid (ampicillin resistant)


text: Engelhardt Institute of Molecular Biology

text: live

text: Generic

text: primary articular chondrocyte

text: breed: New Zealand White; obtained via subtractive hybridization of chondrocytes preincubated with 0.5 mM nitrosoglutathione vs. untreated cells

text: 2 months old

characteristic: dev-stage

text: Oryctolagus cuniculus

text: femoral head

text: Oryctolagus cuniculus femoral head 2 months old


text: Universitaet zu Koeln

characteristic: INSDC center name

text: live

text: Generic

text: Scherffelia dubia

text: SAG 40.89

text: Scherffelia dubia pSPORT1


text: The University of Tokyo, Department of Parasitology

text: live

text: Generic

text: erythrocytic stage

text: Plasmodium falciparum 3D7

text: Sugano Malaria cDNA library


text: Tokyo University of Marine Science and Technology, Graduate School of Marine Science and Technology

text: live

text: Generic

text: leukocytes

text: adult

text: Paralichthys olivaceus

text: lambda ZAPII-Con A stimulated leukocytes


text: Yokohama City University, Division of Biochemistr

text: live

text: Generic

text: HeLa

text: Homo sapiens

text: Homo sapiens HeLa (Suzuki,T.)


text: Univ. of Tokyo, Grad. Sch. of Science

text: live

text: Generic

characteristic: cultivar

text: Zea mays

text: mesophyll cell

text: Zea mays mesophyll cell


text: Univ. of Tokyo, Grad. Sch. of Science

text: live

text: Generic

text: Zea mays

text: Bundle sheath strand

text: Zea mays Bundle sheath strand


text: Hiroshima Univ.

text: live

text: Generic

text: common name:mudskipper

text: Periophthalmus modestus

text: skin

text: Periophthalmus modestus skin


text: University of Tokyo

text: live

text: Generic

text: Doryteuthis pealeii

text: Loligo pealei library (Setou M)


text: Universidade de Sao Paulo

text: live

text: Generic

text: glia

characteristic: cell-line

text: ST1HY24 is a subtracted and normalized cDNA library obtained by selective suppression of polymerase chain reaction. The products were cloned in pUC 18 vector and utilized to find differentially expressed genes under glucocorticoid treatment in multiple tumor lineages.

text: adult

text: XL1 BLUE MRF'

characteristic: lab-host

text: Brain

text: Rattus norvegicus

text: Sma I

text: pUC 18

characteristic: vector


characteristic: External Id

characteristic: INSDC first public

text: live

text: Generic

text: cDNAs were oligo-dT primed and directionally cloned. Staging according to Nieuwkoop and Faber. Library was constructed by N. Garrett and A.M. Zorn, (Wellcome/CRC Institute).

text: DH10B (phage-resistant)

text: Xenopus laevis

text: NotI

text: EcoRI

text: pooled embryos, stage 19-26

text: Wellcome CRC pCS2+ st19-26

text: plasmid (ampicillin resistant)


characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: 1st strand was primed with a Not I - oligo(dT) primer, double-stranded cDNA was cloned into the Not I and Eco RI sites of pBluescript KS+. Library was constructed by C. Hashimoto, Ph.D., in the laboratory of K. Cho, Ph.D. (Department of Developmental and Cell Biology, University of California, Irvine).

text: Xenopus laevis

text: NotI

text: EcoRI

text: gastrula, Li-treated

text: Cho Li treated gastrula

text: phagemid (ampicillin resistant)

text: pBluescript KS+


text: live

text: Generic

characteristic: NCBI submission model

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 2.4 kb. Constructed by Life Technologies.

text: adult

text: DH10B (phage-resistant)

text: spleen

characteristic: organ

text: Xenopus laevis

text: NotI

text: SalI

text: phagemid (ampicillin resistant)


text: live

text: Generic

characteristic: NCBI submission package

text: Size-selected for average insert size 1.2 kb. Library was constructed and donated by M. Kirschner (Harvard Medical School).

text: DH10B (phage-resistant)

text: Xenopus laevis

text: NotI

text: SalI

text: pooled embryos (stage 10-14)

text: Kirschner embryo St10 14

text: plasmid (ampicillin resistant)


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 2.1 kb. Constructed by Life Technologies. Note: This is a Xenopus Gene Collection (XGC) library.

characteristic: description

text: embryo, stage 31-32

text: DH10B (phage-resistant)

text: whole embryo

text: Xenopus laevis

text: NotI

text: SalI

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: cDNAs were oligo-dT primed and directionally cloned. Staging according to Nieuwkoop and Faber. Library was constructed by N. Garrett, K. Ryan and A.M. Zorn, (Wellcome/CRC Institute).

text: DH10B (phage-resistant)

text: Xenopus laevis

characteristic: organism

text: NotI

text: EcoRI

text: head, stage 30

text: Wellcome CRC pRN3 head

text: plasmid (ampicillin resistant)


text: Washington University School of Medicine

text: live

text: Generic

text: The cDNA library was constructed from mRNA isolated from 2 week old etiolated whole seedlings of PI468916. Complementary DNA was synthesized from mRNA using a primer consisting of a poly(dT) sequence with a XhoI restriction site. EcoRI adapters were ligated to the blunt-ended cDNA fragments followed by XhoI digestion. The cDNA fragments were directionally cloned into the EcoRI- XhoI restriction site of the pBluescript vector. The ligated cDNA fragments were transformed into DH10B host cells (Gibco BRL). This library was constructed in the laboratory of Dr. Randy Shoemaker at Iowa state university.

text: Glycine soja

text: EcoRI

text: XhoI

text: Whole seedling, 2 week old, etiolated

characteristic: tissue-type

text: plasmid

text: pBluescript II SK+


text: live

text: Generic

text: The cDNA library was constructed from mRNA isolated from root tissue of 2 week old seedlings for PI468916. Complementary DNA was synthesized from mRNA using a primer consisting of a poly(dT) sequence with a XhoI restriction site. EcoRI adapters were ligated to the blunt-ended cDNA fragments followed by XhoI digestion. The cDNA fragments were directionally cloned into the EcoRI-XhoI restriction site of the pBluescript vector. The ligated cDNA fragments were transformed into DH10B host cells (Gibco BRL). This library was constructed in the laboratory of Dr. Randy Shoemaker at Iowa state university.

text: Glycine soja

text: EcoRI

text: XhoI

text: Root, 2 week seedlings

characteristic: title

text: plasmid

text: pBluescript II SK+


text: live

text: Generic

text: Williams

text: The cDNA library was constructed from mRNA isolated from germinating shoots of the cultivar Williams. The seeds were allowed to germinate for 24 hours prior to harvesting the germinating shoots. Complementary DNA was synthesized from mRNA using a primer consisting of a poly(dT) sequence with a XhoI restriction site. EcoRI adapters were ligated to the blunt-ended cDNA fragments followed by XhoI digestion. The cDNA fragments were directionally cloned into the EcoRI-XhoI restriction site of the pBluescript vector. The ligated cDNA fragments were transformed into DH10B host cells (Gibco BRL). This library was constructed in the laboratory of Dr. Randy Shoemaker at Iowa state university.

text: Glycine max

text: EcoRI

text: XhoI

text: Germinating shoot, 24 hour germination

text: plasmid

text: pBluescript II SK+


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.64 kb. Constructed by Life Technologies.

text: DH10B (T1 phage-resistant)

text: brain

text: Homo sapiens

text: NotI

text: SalI

text: glioblastoma with probably TP53 mutation and without EGFR amplification

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.9 kb. Constructed by Life Technologies.

text: DH10B (T1 phage-resistant)

text: brain

text: Homo sapiens

text: NotI

text: EcoRV

text: anaplastic oligodendroglioma

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: mRNA made from head/neck tissue, cDNA made by oligo-dT priming. Directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 300 bp. Primary library. cDNA Library Preparation: David B. Krizman, Ph.D.

text: normal head/neck tissue

text: Homo sapiens

text: plasmid (ampicillin resistant)


text: live

text: Generic

text: mRNA made from normal nasopharyngeal epithelium, cDNA made by oligo-dT priming. Non-directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 500 bp. Primary library. cDNA Library Preparation: David B. Krizman, Ph.D. REFERENCE: Krizman et al. (1996) Cancer Research 56:5380-5383.

text: nasopharynx

text: Homo sapiens

text: normal epithelium

text: plasmid (ampicillin resistant)


text: live

text: Generic

text: mRNA made from head/neck tumor, cDNA made by oligo-dT priming. Directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 300 bp. Primary library. cDNA Library Preparation: David B. Krizman, Ph.D.

text: head/neck

text: Homo sapiens

text: nasopharyngeal carcinoma

text: plasmid (ampicillin resistant)


text: Lawrence Livermore National Laboratory

text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.54 kb. Constructed by Life Technologies.

text: embryo

characteristic: dev-stage

text: DH10B (T1 phage-resistant)

text: embryo, pluripotent cell line

text: Rattus norvegicus

text: NotI

text: SalI

text: phagemid (ampicillin resistant)


characteristic: INSDC center name

text: live

text: Generic

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCCGGGTTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library is normalized; constructed in the laboratory of M. Bento Soares (University of Iowa).

text: DH10B (phage-resistant)

text: blood

text: Homo sapiens

text: NotI

text: EcoRI

text: lymphocyte

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: nasopharynx

text: Homo sapiens

text: normal epithelium

text: plasmid (ampicillin resistant)


text: live

text: Generic

text: mRNA made from normal colonic mucosa, cDNA made by oligo-dT priming. Directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 300 bp. Primary library. cDNA Library Preparation: David B. Krizman, Ph.D. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383.

text: colon

text: Homo sapiens

text: normal colonic mucosa

text: plasmid (ampicillin resistant)


text: live

text: Generic

text: mRNA made from colonic adenocarcinoma, cDNA made by oligo-dT priming. Directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 300 bp. Primary library. cDNA Library Preparation: David B. Krizman, Ph.D. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383.

text: colon

text: Homo sapiens

text: adenocarcinoma (mucinous component)

text: plasmid (ampicillin resistant)


text: live

text: Generic

text: mRNA made from colonic mucosa, cDNA made by oligo-dT priming. Directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 300 bp. Primary library. cDNA Library Preparation: David B. Krizman, Ph.D. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383.

text: colon

text: Homo sapiens

text: normal colonic mucosa

text: plasmid (ampicillin resistant)


text: live

text: Generic

text: mRNA made from colonic adenoma, cDNA made by oligo-dT priming. Directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 300 bp. Primary library. cDNA Library Preparation: David B. Krizman, Ph.D. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383.

text: colon

text: Homo sapiens

text: tubulovillous adenoma

text: plasmid (ampicillin resistant)


text: Lawrence Livermore National Laboratory

text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.77 kb. Constructed by Life Technologies.

text: DH10B (T1 phage-resistant)

text: brain

text: Homo sapiens

text: NotI

text: SalI

text: glioblastoma without EGFR amplification

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: Cloned unidirectionally. Oligo(dT)-NotI Primer: 5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'. Average insert size 2.5 kb. Library constructed by Yulan Piao at the NIA.

text: Postnatal day 1

text: Mus musculus

text: SalI

text: NotI

characteristic: strain

text: NIA Mouse Newborn Kidney cDNA Library1 (Long)

text: plasmid (ampicillin resistant)

text: pSPORT1 (Gibco/BRL Life Technology)


text: National Institute on Aging/National Institutes of Health

text: live

text: Generic

text: Mouse cDNA project by the Laboratory of Genetics, National Institute on Aging (NIA), Intramural Research Program, NIH (http://lgsun.grc.nia.nih.gov/cDNA). This is a short-transcript enriched cDNA library (Ref. Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]). In brief, double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'- pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 26 ug of total RNA, treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-L. The products were purified by phenol/chloroform and Centricon 100. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pSPORT1 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 1.5 kb. The library was constructed by Yulan Piao(NIA).

text: Newborn

text: Mus musculus

text: SalI

text: NotI

text: Newborn Kidney

text: NIA Mouse Newborn Kidney cDNA Library2 (Short)

text: plasmid (ampicillin resistant)

text: pSPORT1 (Invitrogen)


text: live

text: Generic

text: This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978.

text: Clones arrayed from a variety of cDNA libraries

text: Mus musculus

text: SalI

text: NotI

characteristic: sex

text: NIA Mouse 15K cDNA Clone Set

text: plasmid (ampicillin resistant)


text: Christchurch School of Medicine, University of Otago

text: live

text: Generic

text: Liver cell

characteristic: cell type

text: Library prepared using Stratagene ZAP cDNA synthesis kit, catalogue #200450. country=New Zealand.

text: Adult

text: E.coli

characteristic: lab-host

text: Liver

text: Woodworthia maculata

text: Eco RI

text: Xho I

text: Female

text: Southern Alps species

text: Liver

text: Gecko liver Lambda ZAP cDNA library

text: Phagemid

text: pBluescript phagemid


characteristic: External Id

text: live

text: Generic

text: Kennebec

characteristic: cultivar

text: tissue from germinating potato eyes

text: germinating potato eyes

text: SOLR

text: Solanum tuberosum

text: shoots

text: potato shoots/germinating eyes

text: phagemid

text: pBlueScript SK(-)

characteristic: vector


text: National Institute of Agrobiological Sciences

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: adult

text: Oryzias latipes

text: female/male mixed

text: HNI

text: brain

text: Ol-br-ad cDNA


text: The Institute for Genomic Research (TIGR; www.tigr.org)

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Kennebec

text: Various sizes of sprouting eyes (2 mm to 15 mm) were taken from tubers. The tubers were incubated at 26C in the dark for 2-3 weeks prior to sprouting. The eyes were frozen in liquid nitrogen immediately upon removal from tubers.

text: 12-14 weeks post harvest

text: SOLR

text: Solanum tuberosum

text: sprouting tubers

text: sprouting eyes/shoots

text: phagemid

text: pBlueScript SK(-)


text: United States Department of Agriculture

text: live

text: Generic

text: Subtractive library

characteristic: description

text: larva

text: E. coli TOP10F'

text: Apis mellifera

characteristic: organism

text: RsaI

text: RsaI

text: female

text: ligustica

text: Apis mellifera larval caste mRNA

text: Plasmid


text: Technical University of Munich

text: live

text: Generic

text: suspension cells

text: Picea abies

text: elicitor-induced cDNA library

characteristic: title


text: Institute of Bioorganic Chemistry of Polish Academy of Sciences

text: live

text: Generic

text: Ventus

text: cDNA was prepared from polyA+ enriched RNA from roots with developing nodules harvested seven days post inoculation with Bradyrhizobium sp. WM9 (Lupinus). The cDNA was directionally ligated into the Uni-ZAP XR vector from Stratagene and packaged using Gigapack III Gold packaging extracts. Plasmids containing cDNA inserts were excised from the recombinant lambda-ZAP phage using Ex-Assist helper phage and propagated in SOLR cells.

text: roots with immature nodules harvested seven days post inoculation with Bradyrhizobium sp. WM9 (Lupinus)

characteristic: dev-stage

text: E. coli strain SOLR

text: Lupinus luteus

text: EcoRI

text: XhoI

text: roots with young nodules

characteristic: tissue-type

text: pBluescript SK-


text: Kellogg Eye Center, University of Michigan

characteristic: INSDC center name

text: live

text: Generic

text: Retina

characteristic: organ

text: Homo sapiens

text: Retinal Pigment Epithelium sheets

text: Human Retinal Pigment Epithelium cDNA

text: Phage


text: University of Edinburgh

text: live

text: Generic

text: Constructed by David Knox, Moredun Institute, Edinburgh. Primary library titre was 2.0 X 10^5 pfu/ml.

text: Adult

text: Haemonchus contortus

text: EcoRI

text: XhoI

text: Mixed

characteristic: sex

text: Moredun

characteristic: strain

text: Haemonchus contortus adult from David Knox

text: Phage

text: Lambda UNI-Zap XR


characteristic: External Id

text: Clemson University

text: live

text: Generic

text: Small expanding leaves from the growing tip were taken from greenhouse plants (4-6wks old TA496). Tissue was immediately frozen in liquid nitrogen.

text: developing shoots from 4-6wks old plants

text: SOLR

characteristic: lab-host

text: Solanum lycopersicum

text: shoot/meristem

text: tomato shoot/meristem

text: pBluescript SK(-)


text: Clemson University

text: live

text: Generic

characteristic: cultivar

text: Four wk old greenhouse plants were stab inoculated on stem with Agrobacterium tumefaciens C58 (Dr. T.J. Burr, Cornell U.). Galls were allowed to develop for another 4 wks, when gall tissue was frozen in liquid nitrogen.

text: crown galls from full-grown plants (8 wks old)

text: SOLR

text: Solanum lycopersicum

text: crown gall

text: tomato crown gall

text: pBluescript SK(-)

characteristic: vector


text: Clemson University

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Pollen was collected from open flowers from L.pennellii TA56, and stored at -80 C until library construction.

text: pollen collected from open flowers

text: SOLR

text: Solanum pennellii

text: pollen

text: wild tomato pollen

text: pBluescript SK(-)


text: University H. Poincare- NANCY I

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: The cDNA was prepared by a RACE protocol with RNAs extracted from Paxillus involutus treated with cadmium.

text: Paxillus involutus

text: ATCC 200175

text: Cadmium treated Paxillus involutus mycelia


text: Harvard University, Howard Hughes Medical Institute

text: live

text: Generic

characteristic: NCBI submission package

text: Library constructed using SuperScript Plasmid Library kit (Life Technologies). cDNA made by oligo-dT priming. Xho I site destroyed during cloning. Size-selected by column fractionation; average insert size 1.59 kb. Primary library, unamplified.

characteristic: description

text: Adult

text: Pancreas

text: Homo sapiens

text: Not I

text: Xho I

text: Both

text: Islets of Langerhans

text: Melton Human Islets HIZ1

text: plasmid (kanamycin resistant)


text: live

text: Generic

text: Library constructed using SuperScript Plasmid Library kit (Life Technologies). cDNA made by oligo-dT priming. Xho I site destroyed during cloning. Size-selected by column fractionation; average insert size 1.1 kb. Primary library, unamplified.

text: Adult

text: Pancreas

text: Mus musculus

characteristic: organism

text: Not I

text: Xho I

text: Male

text: ICR

text: Islets of Langerhans

text: Melton Mouse Islets MIZ1

characteristic: title

text: plasmid (kanamycin resistant)


text: live

text: Generic

text: Library constructed using SuperScript Plasmid Library kit (Life Technologies). cDNA made by oligo-dT priming. XhoI site destroyed during cloning. Size-selected by column fractionation; average insert size 1.2kb. Primary library, unamplified.

text: Embryonic day 16.5

characteristic: dev-stage

text: Pancreas

text: Mus musculus

text: NotI

text: XhoI

text: Both

text: ICR

text: Total pancreas

characteristic: tissue-type

text: Melton Mouse E16 5 Pancreas Library M16Z1

text: plasmid (kanamycine resistant)


characteristic: INSDC center name

text: live

text: Generic

text: Library constructed using SuperScript Plasmid Library kit (Life Technologies). cDNA made by oligo-dT priming. Xho I site destroyed during cloning. Size-selected by column fractionation. Primary library, unamplified.

text: Adult

text: Pancreas

characteristic: organ

text: Mus musculus

text: Not I

text: Xho I

text: Male

text: ICR

text: Total Pancreas

text: Melton Mouse Adult Pancreas 1

text: plasmid (kanamycin resistant)


text: live

text: Generic

text: Adult

text: Pancreas

text: Mus musculus

text: Not I

text: Xho I

text: Male

characteristic: sex

text: ICR

characteristic: strain

text: Total Pancreas

text: Melton Mouse Adult Pancreas 2

text: plasmid (kanamycin resistant)


characteristic: External Id

text: live

text: Generic

text: Anchor enzyme: NlaIII~ Library constructed in the laboratory of P. Morin, Ph.D. (National Institute on Aging).

characteristic: lab-host

text: skin

text: Homo sapiens

text: primary mesothelioma (pure epithelial)

text: plasmid


text: live

text: Generic

text: Anchor enzyme: NlaIII~Invasive ductal carcinoma - primary tumor. Age at discovery 52.8 years, 16 postive lymph nodes, 3.2 cm primary tumor, histo grade = 3, nuclear grade = 3, ER+, PR+. Library constructed in the laboratory of K. Polyak, M.D., Ph.D. (Dana Farber Cancer Institute).

text: breast

text: Homo sapiens

text: female

text: tumor

text: plasmid

characteristic: vector


characteristic: INSDC first public

text: live

text: Generic

text: Anchor enzyme: NlaIII~Invasive ductal carcinoma - primary tumor. Age at discovery 56.5 years, 10 postive lymph nodes, 3 cm primary tumor, histo grade = 3, nuclear grade = 3, ER-, PR-. Library constructed in the laboratory of K. Polyak, M.D., Ph.D. (Dana Farber Cancer Institute).

text: breast

text: Homo sapiens

text: female

text: tumor

text: plasmid


characteristic: INSDC last update

text: live

text: Generic

text: Anchor enzyme: NlaIII~Metastatic tumor (lymph node metastasis from breast primary tumor). Age at discovery of primary tumor: 56.5 years, 10 postive lymph nodes, Primary tumor is 3 cm, histo grade = 3, nuclear grade = 3, ER-, PR-. Library constructed in the laboratory of K. Polyak, M.D., Ph.D. (Dana Farber Cancer Institute).

text: lymph node

text: Homo sapiens

text: female

text: metastasis from breast tumor

text: plasmid


text: University of Sao Paulo - Faculty of Medicine

text: live

characteristic: INSDC status

text: Generic

text: cDNA fragment cloned from DDRT-PCR screen of ecdysteroid (makisterone A)-regulated genes in the ovary of last instar honey bee worker larvae

text: XL-1 Blue

text: larval ovary

text: Apis mellifera

text: EcoR I

text: Pst I

text: female - worker

text: carnica

text: ovary

text: Apis mellifera ovary DDRT-PCR

text: pUC 18


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

characteristic: NCBI submission model

text: Cloned unidirectionally; oligo-dT primed. Average insert size 1.3 kb. Library enriched for full-length clones and constructed by Life Technologies. Note: this is a NIH_MGC Library.

text: DH10B (phage-resistant)

text: kidney

text: Homo sapiens

text: NotI

text: SalI

text: hypernephroma, cell line

text: phagemid (ampicillin resistant)


text: Indian Institute of Horticultural Research

text: live

text: Generic

characteristic: NCBI submission package

text: Alphonso

characteristic: cultivar

text: It is a cDNA product (obtained by RT-PCR) which was differentially expressed when amplified using two non-specific primers

text: Full ripe stage

text: Fruit

text: Mangifera indica

text: Xba I

text: Bam H I

text: Hermaphrodite

text: Pulp

text: Mango fruit cv. Alphonso cDNA

text: Plasmid

text: PUC 57


text: live

text: Generic

text: Totapuri

characteristic: description

text: 3/4th ripe stage

text: Fruit

text: Mangifera indica

characteristic: organism

text: Xba I

text: Bam H I

text: Hermaphrodite

text: Pulp

text: Mango fruit cv. Totapuri cDNA

text: Plasmid

text: PUC 57


text: Institute for Cancer Research, The Norwegian Radium Hospital

text: live

text: Generic

text: Human Osteosarcoma

characteristic: cell type

text: Saos-2 (ATCC HTB-85)

characteristic: cell-line

text: Homo sapiens

text: Saos-2 cDNA library

characteristic: title

text: Not cloned


text: Wuhan University

characteristic: INSDC center name

text: live

text: Generic

text: Using the SMART cDNA synthesis and long distance PCR amplification strategy, a cDNA library from mRNA of a 29-day-old chorionic-free human embryo was constructed. The library's complexities is 6.3x106 clones with a average insert size of 1.1kb.

text: 4-week-old human embryo timed post-conception

characteristic: dev-stage

text: embryo

characteristic: organ

text: Homo sapiens

text: 4-week-old human embryo cDNA library


characteristic: External Id

text: Washington University School of Medicine

text: live

text: Generic

text: Williams

text: The cDNA library was constructed from mRNA isolated from immature pods (approximately 2cm long) of greenhouse grown plants. The library was prepared using the Life Technologies pSuperScript cDNA library construction kit. Complementary DNA was synthesized from mRNA using a poly(dT) sequence with a NotI restrictions site. SalI linkers adapters were ligated to the blunt-ended cDNA fragments followed by NotI digestion. The cDNA fragments were directionally cloned into the NotI-SalI restriction site of the pSPORT1 vector. The ligated cDNA fragments were transformed into E.coli ElectroMax DH10B host cells. This library was constructed in the laboratory of Dr. Lila Vodkin by Anu Khanna at the University of Illinios at Urbana-Champaign. email: l-vodkin@uiuc.edu

text: Glycine max

text: NotI

text: SalI

text: immature pods (~2cm long) of greenhouse grown plants

characteristic: tissue-type

text: plasmid


text: Miyazaki University

text: live

text: Generic

text: common name:five-ray yellowtail ; infected with iridovirus

text: Seriola quinqueradiata

text: head kidney

text: Seriola quinqueradiata Head kidney infected with Iridovirus


text: Yamanashi Medical University School of Medicine

text: live

text: Generic

text: mouse jejunum

text: adult

text: Mus musculus

text: male

characteristic: sex

characteristic: strain

text: whole lumen

text: Mus musculus whole lumen WBB6F1-W/WV adult


text: University of Tsukuba

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Shimoshirazu-jibai

text: 18-day-old seedling

text: Cucumis sativus

text: Apex

text: Cucumis sativus Apex 18-day-old seedling


text: Athersys, Inc.

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: See 'Creation of Genome-wide Protein Expression Libraries using Random Activation of Gene Expression', Nature Biotechnology, in press. Note that even though the cell type indicated is HT1080, since a random activation method was used, these sequence tags are not necessarily expressed in HT1080 under normal circumstances.

text: Homo sapiens

text: Athersys RAGE Library


text: live

text: Generic

characteristic: NCBI submission package

text: SL2-Oligo(dT) PCR-based library. Meloidogyne javanica cDNA PCR products of size >400 nucleotides containing SL2 on the 5' end and oligo(dT) on the 3' end were non-directionally cloned into pCRII-TOPO (Invitrogen) following the Topo TA cloning protocol. The cDNA insert can be excised by digestion with EcoRI. The library was constructed by Dr. Andrew Kloek at Divergence LLC and Brandi Chiapelli and Dr. James McCarter at Washington University, St. Louis. Nematodes were provided by Dr. David Bird of North Carolina State University.

text: enriched for eggs

characteristic: lab-host

text: Meloidogyne javanica

text: Meloidogyne javanica Egg SL2 Topo2 Chiapelli Kloek McCarter

text: Ampicillin and Kanamycin resistant

text: pCRII-TOPO

characteristic: vector


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: Cloned unidirectionally; oligo-dT primed. Average insert size 2.5 kb. Library enriched for full-length clones and constructed by Life Technologies. Note: this is a NIH_MGC Library.

characteristic: description

text: DH10B (phage-resistant)

text: testis

text: Homo sapiens

characteristic: organism

text: NotI

text: SalI

text: embryonal carcinoma, cell line

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: Cloned unidirectionally; oligo-dT primed. Average insert size 1.7 kb. Library enriched for full-length clones and constructed by Life Technologies. Note: this is a NIH_MGC Library.

text: DH10B (phage-resistant)

text: bladder

text: Homo sapiens

text: NotI

text: SalI

text: transitional cell papilloma, cell line

characteristic: title

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: Cloned unidirectionally; oligo-dT primed. Average insert size 3.3 kb. Library enriched for full-length clones and constructed by Life Technologies. Note: this is a NIH_MGC Library.

text: DH10B (phage-resistant)

text: eye

text: Mus musculus

text: NotI

text: SalI

text: retina

text: phagemid (ampicillin resistant)


characteristic: INSDC center name

text: live

text: Generic

text: The library was constructed using mRNA isolated from total RNA with oligo-dT cellulose. Total RNA was generated from muscle larvae that were isolated from infected rats. Larvae were liberated by pepsin/HCl digestion, incubated with 1% SDS, treated with RNase and DNase to eliminate host nucleic acid contamination, and purified on a Percoll gradient. The T. spiralis isolate was obtained from Dickson Despommier (Columbia University). The library was provided by Dr. Doug Jasmer (djasmer@vetmed.wsu.edu) and colleagues at Washington State University. DNA Sequencing by: Washington University Genome Sequencing Center St. Louis.

text: muscle stage larvae

text: Trichinella spiralis

text: NotI

text: SalI

text: Trichinella spiralis ML CMVsport jasmer

text: plasmid (ampicillin resistant)


text: live

text: Generic

text: Merozoites were obtained from ceacal scrapings of chickens infected with E. tenella. cDNA was synthesized from poly mRNA using an oligo-dT primer containing a XhoI site. Following second strand synthesis, EcoRI adapters were ligated to the cDNA and products were size-selected on Sephacryl S500. The cDNAs were ligated to EcoRI/XhoI prepared lambda ZAPII(Stratagene). Clones were converted to phagemids by mass excision using ExAssist helper phage and E.coli SOLR cells (Stratagene). Clones were selected by negative hybridization against a pool of over-represented ESTs (N>=10, from 1506 previous reads), and transformed to DH10B (GENEHOGS, STRATAGENE). The library may contain a small percentage of host or bacterial contaminents.

text: Merozoite

characteristic: dev-stage

text: DH10B (GENEHOG, E.coli HS996)

text: Eimeria tenella

text: EcoRI

text: XhoI

text: Eimeria tenella M5-6 cDNA Neg Selected

text: phagemid (Amp. resistant)

text: Bluescript SK-


text: Washington University School of Medicine

text: live

text: Generic

text: Sporozoites were obtained from in vitro sporulated and excysted oocysts of E.tenella grown in chickens. cDNA was synthesized from poly mRNA using an oligo-dT primer containing a XhoI site. Following second strand synthesis, EcoRI adapters were ligated to the cDNA and products were size-selected on sephacryl S500. The cDNA were ligated to EcoRI/XhoI prepared lambda ZapII (Stratagene). Clones were converted to phagemids by mass excision using ExAssist helper phage and E.coli SOLR cell (Stratagene). Clones were selected by negative hybridization against a pool of overrepresented ESTs (N>=10,from 1682 previous reads). Insert sizes range from 1.2-2.9Kb. The library may contain a small percentage of host or bacterial contaminents.

text: Sporozoite stage

text: SOLR

text: Eimeria tenella

text: EcoRI

text: XhoI

text: Eimeria tenella S5-2 cDNA Neg Selected

text: phagemid (Amp. resistant)

text: Bluescript SK-


text: Genoscope - Centre National de Sequencage

text: live

text: Generic

text: 1st strand cDNA was primed with a NotI-oligo(dT) primer. Five prime end enriched, double-stranded cDNA was digested with Not I and cloned into the Not I and Eco RV sites of the pCMVSPORT 6 vector. Library was normalized. Library was constructed by Life Technologies. Contact : Feng Liang Life Technologies, a division of Invitrogen 9800 Medical Center Drive Rockville, Maryland 20850, USA Fax : (1) 301 610 8371 Email : fliang@lifetech.com URL : http://fulllength.invitrogen.com

text: Homo sapiens

text: NotI

text: placenta

text: pCMVSPORT 6


text: live

text: Generic

text: 1st strand cDNA was primed with a NotI-oligo(dT) primer. Five prime end enriched, double-stranded cDNA was digested with Not I and cloned into the Not I and Eco RV sites of the pCMVSPORT 6 vector. Library was constructed by Life Technologies. Contact : Feng Liang Life Technologies, a division of Invitrogen 9800 Medical Center Drive Rockville, Maryland 20850, USA Fax : (1) 301 610 8371 Email : fliang@lifetech.com URL : http://fulllength.invitrogen.com

text: Homo sapiens

text: NotI

text: male

text: Adult brain

text: pCMVSPORT 6


text: live

text: Generic

text: Homo sapiens

text: NotI

text: male

text: T cells from T cell leukemia

text: pCMVSPORT 6


text: live

text: Generic

text: Homo sapiens

text: NotI

text: male

text: B cells from Burkitt lymphoma

text: pCMVSPORT 6


text: Fudan University

text: live

text: Generic

text: salt stressed Dunaliella salina subtractive library

text: Dunaliella salina


text: National Institute of Allergy and Infectious Diseases

text: live

text: Generic

text: Filarial nematode parasite of humans. RNA was prepared from 7,000 third stage infective larvae of Loa loa isolated fromChrysops flies 14 days after feeding on infected rhesus monkeys. Total RNA was converted to cDNA using reverse transcriptase and oligo (dT) followed by RNase H and DNApol I. The unamplified library contained 5 x 105 pfu (106 pfu/ml) with an average insert size of 1 kb. L3 larvae were provided by Dr. Robert Lowrie and the library was constructed by Amy Klion and Michelle Lizotte-Waniewski. The library is available from Amy Klion, email aklion@nih.gov

text: infective L3 larvae

text: Rhesus monkey

text: Loa loa

text: EcoR I

text: Xho I

text: Cameroon

text: lambda Unizap XR


text: Institut National de la Recherche Agronomique

text: live

text: Generic

text: Shiraz

characteristic: cultivar

text: Oriented library, construction described in Generation of ESTs from grape Berry (skin, pulp or seeds) at various developmental stages by Terrier,N., Ageorges,A., Abbal,P., Romieu,C. in J. Plant Physiol. 158 (12): 1575-83 2001

text: green stage

text: Fruit

characteristic: organ

text: Vitis vinifera

text: Eco RI

text: XhoI

text: Green Grape berries Lambda Zap II Library

text: Phage

text: Lambda Zap II


characteristic: External Id

text: live

text: Generic

text: Shiraz

text: ripening stage

text: Fruit

text: Vitis vinifera

text: Eco RI

text: XhoI

text: Ripening Grape berries Lambda Zap II Library

text: Phage

text: Lambda Zap II


text: live

text: Generic

text: Shiraz

text: Oriented library

text: veraison stage

text: Fruit

text: Vitis vinifera

text: Eco RI

text: XhoI

text: 'Veraison' Grape berries Lambda Zap II Library

text: Phage

text: Lambda Zap II


text: Advanced Center for Genome Technology, University of Oklahoma

text: live

text: Generic

text: 5' end of cDNA cloned into EcoRI site of pBluescript; 3' end of cDNA cloned into XhoI site of pBluescript

text: sexual

text: E. coli strain SOLR

text: Neurospora crassa

text: EcoRI

text: XhoI

text: wild type

text: perithecia (fruiting bodies)

characteristic: tissue-type

text: Neurospora crassa sexual cDNA library, Uni-zap vector system

text: phagemid

text: pBlueScript SK-


text: live

text: Generic

text: SL1-Oligo(dT) PCR-based library. Strongyloides_ratti_L1 cDNA PCR products of size >400 nucleotides containing SL1 on the 5' end and oligo(dT) on the 3' end were non-directionally cloned into pCRII-TOPO (Invitrogen) following the Topo TA cloning protocol. The cDNA insert can be excised by digestion with EcoRI. The library was constructed by Brandi Chiapelli and Dr. James McCarter at Washington University, St. Louis. Nematodes were provided by Dr. Mark Viney of the University of Bristol, UK.

text: Strongyloides ratti

text: Strongyloides ratti L1 SL1 Topo1 Chiapelli McCarter

text: plasmid (amp. or kan. resistant)

text: pCRII-TOPO


text: Washington University School of Medicine

text: live

text: Generic

text: SL1-Oligo(dT) PCR-based library. Strongyloides_ratti_L3_adult cDNA PCR products of size >400 nucleotides containing SL1 on the 5' end and oligo(dT) on the 3' end were non-directionally cloned into pCRII-TOPO (Invitrogen) following the Topo TA cloning protocol. The cDNA insert can be excised by digestion with EcoRI. The library was constructed by Brandi Chiapelli and Dr. James McCarter at Washington University, St. Louis. Nematodes were provided by Dr. Mark Viney of the University of Bristol, UK.

text: Strongyloides ratti

text: Strongyloides ratti L3 adult SL1 Topo1 Chiapelli McCarter

text: plasmid (amp. or kan. resistant)

text: pCRII-TOPO


text: Bowling Green State University

text: live

text: Generic

text: zoospore

text: Phytophthora sojae

text: P6497, Race 2

characteristic: strain

text: Phytophthora sojae cDNA


characteristic: INSDC first public

text: live

text: Generic

text: The library was constructed by Brandi Chiapelli and Dr. James McCarter at Washington University, St. Louis. The cDNA was made by using Dynabead oligo-dT priming (Dynal). PCR based library using a modified protocol from the SMART PCR cDNA Synthesis Kit from Clontech. Directionally cloned into the UDG sites of pAMP1. Nematodes are the OP25 strain. Frozen J2 nematodes were provided by Dr. Rick Davis of North Carolina State University. Special thanks to Dr. Yulia Korshunova from the Lovett Lab at Washington University in St. Louis for helping with methods

text: enriched for 2nd stage juveniles

text: Heterodera glycines

text: Heterodera glycines J2 pAMP1 v2.1 Chiapelli McCarter

text: plasmid (ampicillin resistant)


text: Harvard University, Howard Hughes Medical Institute

characteristic: INSDC last update

text: live

text: Generic

text: Library constructed using SuperScript Plasmid Library kit (Life Technologies). cDNA made by oligo-dT priming. Xho I site destroyed during cloning. Size-selected by column fractionation. Primary library, unamplified.

text: Day 1 through day 4 postnatal, mixed

text: Pancreas

text: Mus musculus

text: Not I

text: Xho I

text: Both

characteristic: sex

text: ICR

text: Total Pancreas

text: Melton Mouse Newborn Pancreas

text: plasmid (kanamycin resistant)


text: US Department of Agriculture, Agriculture Research Service, Pacific West Area, Western Regional Research Center

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Chinese Spring

text: Total RNA was prepared from 3-6 DAP seed (Gustafson), equal portions of RNA were pooled from these four DAPs, polyA was purified, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab at the University of California, Riverside (Akhunov, Chin, Choi, Close, Fenton, Kianian, Otto, Simons, Zhang). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

text: Adult plant

text: E. coli SOLR

text: Triticum aestivum

text: EcoRI

text: XhoI

text: Young seed

text: Wheat 3-6 DAP seed cDNA library

text: Phagemid

text: Lambda Uni-ZAP XR, excised phagemid


text: live

text: Generic

characteristic: NCBI submission package

text: Chinese Spring

text: Plants were grown under hydroponic conditions at UC Davis, salt stressed for 12 hours, and for 7 days, then dissected and frozen (Akhunov in J Dvorak Lab). Total RNA was prepared from sheath tissue, equal quantities of RNA were pooled from the two samples, polyA was purified from the pooled RNA, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab at the University of California, Riverside (Akhunov, Chin, Choi, Close, Fenton, Kianian, Otto, Simons, Zhang). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

text: Adult plant

text: E. coli SOLR

characteristic: lab-host

text: Triticum aestivum

text: EcoRI

text: XhoI

text: Sheath

text: Wheat salt-stressed sheath cDNA library

text: Phagemid

text: Lambda Uni-ZAP XR, excised phagemid


text: live

text: Generic

text: The tissue, total RNA, and poly(A) RNA were prepared from shoot apex at double-ridge stage to terminal-spikelet stage, the vegetative shoot apex was induced to flower, a cDNA library was made, and the cDNA clones were in vivo excised at the University of California, Davis (V. Echenique, B. Stamova, J. Dubcovsky). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

characteristic: description

text: Seven week-old plants

text: E. coli XLOLR

text: Triticum monococcum

text: EcoRI

text: XhoI

text: Shoot apex

text: Triticum monococcum induced shoot apex cDNA library

text: Phagemid

text: Lambda Uni-ZAP XR, excised phagemid

characteristic: vector


text: KTH

text: live

text: Generic

characteristic: cell-line

text: Shotgun cloning of RDA difference products. Macrophage and foamcell libraries were submitted to successive rounds of subtractive hybridisations generating populations of gene fragments that are differentially expressed in macrophage to foam cell formation.

text: Homo sapiens

characteristic: organism

text: BamHI

text: BamHI

text: Human THP1 cell line library

characteristic: title

text: Plasmid


text: Henan Medical University

characteristic: INSDC center name

text: live

text: Generic

text: Homo sapiens

text: Human carcinoma of esophagus


text: North Carolina State University

text: live

text: Generic

text: Planings (secondary)

characteristic: cell type

text: The library is from early (spring) secondary wood, taken from a ten year old tree in the transitional phase. The tree is a kind gift of the Westvaco Corporation. Secondary xylem was harvested from the tree by peeling back the bark and primary xylem and then removing the underlying tissue with a block plane. NOTE: The sequences contain a 'cDNA adapter' between the EcoRI site and the start of the EST. The adapter sequence is 'AATTCGGCACGAG'.

text: Transitional

characteristic: dev-stage

text: Pinus taeda

text: Eco RI

text: XhoI

text: Coastal plain loblolly pine from North Carolina

text: Xylem

text: NXPV (Nsf Xylem Planings wood Vertical)

text: BlueScript SK


characteristic: External Id

text: United States Army Medical Research Institute of Chemical Defense (USAMRICD)

text: live

text: Generic

text: primary keratinocyte

text: Clonetics 4075

text: Library preparation: Sequence isolated using PCR-select (Clontech) subtraction library construction (Diatchenko et al., (1996) Proc. Natl. Acad. Sci. USA 93: 6025-6030) after sulfur mustard exposure of primary human adult epidermal keratinocytes. Subtraction library cloned for random sequence selection into pT-Adv vector.

text: adult

text: E. coli (ampicillin-resistant)

text: Homo sapiens

text: female

text: skin

characteristic: tissue-type

text: Human Keratinocyte Subtraction Library- Downregulated Transcripts

text: Plasmid

text: pT-Adv


text: Michigan State University

characteristic: INSDC first public

text: live

text: Generic

text: Montmorency

characteristic: cultivar

text: PRA and PRB libraries were constructed with mRNA extracted from fruits harvested 44 days after pollination. YGC and YGD libraries were constructed from mRNA extracted from fruits 8 days after pollination.

text: 8 days or 44 days after pollination

text: Fruit

characteristic: organ

text: Prunus cerasus

text: sour cherry fruit library

text: pBluescriptSK


text: Agriculture and Agri-food Canada

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Plants were grown for 1 month at 20oC/16 hrs light/day (average 8 leaves, 1 cm tall, weight 0.02g/plant). Then they were exposed to 2oC, 12 hrs light/day, for 1 week. Library prepared by C. Piche using Stratagene kit.

text: 1-month seedlings - 1 cm tall - 8 leaf

text: Descurainia sophia

text: Eco RI

text: Xho I

text: leaf, stem

text: Ds01_AAFC_ECORC_cold_stressed_Flixweed_seedlings

text: Plasmid

text: Bluescript SK+/XhoI-EcoRI


text: Agriculture and Agri-food Canada

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Corn seedlings at 4-leaf stage were exposed to low temperature/high light (10oC/700-800uE/m2/s) for 4 days. Plants were grown/treated by J. Simmonds/L. Cass. Library prepared by C. Piche using Stratagene kit.

text: Zea mays

text: Eco RI

text: Xho I

text: Leaf, crown

text: Zm03_AAFC_ECORC_cold_stressed_maize_seedlings

text: Plasmid

text: Bluescript SK+/XhoI-EcoRI


text: Agriculture and Agri-food Canada

text: live

text: Generic

text: Lower temperature 5o C / hour from 22 to 12oC; bring to 5o in 1 hour from 12oC. Leave at 5oC 2 days, photoperiod 16 hours. Light intensity was 125 uE-1. Library prepared by in vivo mass excision from amplified library.

characteristic: description

text: Zea mays

text: Eco RI

text: Xho I

text: Leaf, crown

text: Zm04_AAFC_ECORC_cold_stressed_maize_seedlings

text: Plasmid

text: Bluescript SK-/XhoI-EcoRI


text: Iowa State University

text: live

text: Generic

text: The MI-P-E1 library is derived from whole fetus at gestational day 20. First strand cDNA was primed with a Not I - oligo(dT) primer [5'GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT3'], double-stranded cDNA was size selected, ligated to SalI adapters (BRL), digested with Not I and cloned into the Not I and SalI sites of the pSPORT1 vector (BRL). For a detailed description of the library from which this clone was derived, please visit our web site at http://pigest.genome.iastate.edu/.

text: DH10B (Life Technologies)

characteristic: lab-host

text: Sus scrofa

text: Sal I

text: Not I

text: large white outbred

characteristic: strain

text: pSPORT1 (BRL)

characteristic: vector


text: Oklahoma State University

text: live

text: Generic

text: Jackpot

text: Clontech PCR-select cDNA subtraction library

text: E.coli

text: Cynodon dactylon

characteristic: organism

text: EcoR I

text: EcoR I

text: Crown

text: Bermudagrass/SDS SSH Library

characteristic: title

text: plasmid

text: pCR 2.1-TOPO


text: live

text: Generic

text: Anchor enzyme: NlaIII~Metastatic tumor (lymph node metastasis from breast primary tumor). Age at discovery of primary tumor: 52.8 years, 16 postive lymph nodes. Primary tumor is 3.2 cm, histo grade = 3, nuclear grade = 3, ER+, PR+. Library constructed in the laboratory of K. Polyak, M.D., Ph.D. (Dana Farber Cancer Institute).

text: lymph node

text: Homo sapiens

text: female

characteristic: sex

text: metastasis from breast tumor

text: plasmid


text: live

text: Generic

text: Anchor enzyme: NlaIII~ Library constructed in the laboratory of P. Morin, Ph.D. (National Institute on Aging).

text: peritoneum

text: Homo sapiens

text: mesothelial cells (90%)

text: plasmid


text: Yamaguchi University

characteristic: INSDC center name

text: live

text: Generic

text: synonym:Brassica campestris

text: Brassica rapa

text: root

text: Brassica rapa root


characteristic: External Id

text: Pusan National University

text: live

text: Generic

text: SiHa

characteristic: cell-line

text: Homo sapiens

text: Homo sapiens SiHa


text: Roslin Institute

characteristic: INSDC first public

text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Uni-ZAP XR vector. Average insert size: 1.5kb.; 5' adaptor sequence: 5' GAATTCGGCACGAG 3'; 3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'

text: 5 days old

characteristic: dev-stage

text: SOLR cells (kanamycin resistant)

text: Gallus gallus

text: Embryo

characteristic: tissue-type

text: Stratagene Chick Embryo Lambda cDNA Library (* 937405)

text: pBLUESCRIPT SK


text: Roslin Institute

characteristic: INSDC last update

text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. 5' adaptor sequence: 5' TCGACCTCGAG 3'; 3' adaptor sequence: 5' GCGGCCGCTTTTTTTTTTTTTTTTTT 3'

text: 5 days old

text: Gallus gallus

text: Embryo

text: BP Chicken Embryo Library

text: pBLUESCRIPT SK


text: University of New Brunswick

text: live

characteristic: INSDC status

text: Generic

text: Nicotiana tabacum

text: Nicotiana tabacum SR1


text: Birmingham University

text: live

text: Generic

characteristic: NCBI submission model

text: Platichthys flesus

text: ovary

text: Platichthys flesus ovary


text: Birmingham University

text: live

text: Generic

characteristic: NCBI submission package

text: Platichthys flesus

text: liver

text: Platichthys flesus liver


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: cDNA oligo-dT primed using oligo 5'-GAGAGAGAGAGGATCCAATACTGGAGAGTTTTTTTTTTTTTTTTVN-3' and directionally cloned into XhoI and BamHI sites using the 5' adaptor 5'-AGAGAGAGAGCTCGAGCTCTAATAAGGTGACACTATAGAACCA-3'. Size selection was done automatically by the cloning vector (lambda FLC-II, P. Carninci et al., unpublished). Library was amplified at the phage stage and normalized (RoT value = 5). This library was constructed using the CapTrapper method and Trehalose thermoactivated reverse transcriptase to enrich for full-length clones. Library constructed by Piero Carninci (RIKEN, Genome Science Laboratory, Japan: http://genome.rtc.riken.go.jp/) using egg RNA supplied by Marc Kirschner (Harvard Medical School, Dept. of Cell Biology). References: Methods Enzymol.303:19-44 and Genome Res. 10:1617-1630 Please contact Marc Kirschner (marc@hms.harvard.edu) for information on obtaining aliquots of this cDNA library.

characteristic: description

text: Xenopus laevis

text: BamHI

text: XhoI

text: egg (unfertilized)

text: RIKEN Xenopus egg

text: phagemid (ampicillin resistant)

text: pCS2G (custom)


text: live

text: Generic

text: cDNAs were oligo-dT primed and directionally cloned. Staging according to Nieuwkoop and Faber. Library was constructed by N. Garrett, P. LeMaire, A.M. Zorn, and J.B. Gurdon (Wellcome/CRC Institute).

text: DH10B (phage-resistant)

characteristic: lab-host

text: Xenopus laevis

text: NotI

text: EcoRI

text: pooled embryos, stage 24-26

text: Wellcome CRC pcDNAI St24-26

text: plasmid (ampicillin resistant)

text: pcDNAI


text: National Cancer Institute / NIH

text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 2.1 kb. Constructed by Life Technologies. PLEASE NOTE: This library contains high levels of contamination by mouse clones

text: DH10B (phage-resistant)

text: Xenopus laevis

text: NotI

text: SalI

text: embryo, stage 17/19

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.6 kb. Constructed by Life Technologies.

text: DH10B (phage-resistant)

text: heart

characteristic: organ

text: Xenopus laevis

text: NotI

text: SalI

text: phagemid (ampicillin resistant)


text: Maastricht University

text: live

text: Generic

text: Suppression subtractive hybridization library. Comparison of human ruptured atherosclerotic plaque mRNA with human stable atherosclerotic plaque mRNA

text: advanced stage atherosclerosis

text: vasculature

text: Homo sapiens

text: female, male

text: aorta

text: Homo sapiens atherosclerosis related cDNA library

text: Plasmid

text: pGEMT-easy

characteristic: vector


text: Kansas State University

text: live

text: Generic

text: Solanum lycopersicum

characteristic: organism

text: leaves overexpressing Pto

text: Tomato leaves overexpressing Pto


text: Roslin Institute

text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. 5' adaptor sequence: 5' TCGACCTCGAG 3' ; 3' adaptor sequence: 5' GCGGCCGCTTTTTTTTTTTTTTTTTT 3' Poly A RNA purchased from Clonetech (*6854-1)

text: Gallus gallus

text: Brain

text: BP Chicken Brain Library

characteristic: title


text: Incyte Genomics

text: live

text: Generic

text: placenta

text: Homo sapiens

text: Human placenta cDNA expression library