characteristic: External Id

text: Universidade Estadual de Campinas

text: live

text: Generic

text: F-352 near isogenic line

characteristic: cultivar

text: Plant Material and RNA Isolation: Field grown maize plants from inbred line F352 were used. Ears were harvested at 10, 15, 20 and 25 days after pollination (DAP), seeds were cut from the ear and the upper third of the endosperms, containing only endosperm, aleurone and pericarpal tissues, was removed, frozen in liquid nitrogen and stored at -80o C. Frozen endosperms were pulverized in liquid nitrogen and total RNA was isolated according the method of Manning (9). Poly(A)+RNA was isolated using Oligotex-dT. cDNA libraries were constructed using SuperScript Plasmid System for cDNA Synthesis and Plasmid Cloning Kit as described in Vettore, et al., (2001) The libraries that made SUCEST. Genet Mol Biol 24: 1-7. cDNAs ranging from 500 to 800 bp in size were assigned as short libraries (S10, S15, S20), and cDNAs >800 were assigned as long libraries (L10, L15, M15, N15, L20, L25). Unamplified libraries were plated and individual colonies picked and transferred to 96 well plates containing liquid Circle Grow (CG) medium supplemented with 100 mg/L of ampicillin and 8% glycerol. Three copies of each cDNA clone were stored at -80o C. Additional information can be found in : Verza, N.C., Silva, T.R., Cord-Neto, G., Nogueira, F.T.S, Fisch, P.H., De Rosa Jr., V.E., Martins, M.M., Vettore, A.L., da Silva, F.R. and Arruda, P. (2005) Endosperm-preferred expression of maize genes as revealed by transcriptome-wide analysis of expressed sequence tags. Plant Molecular Biology (/in press/)

text: multiple stages (10 to 25 days after pollination, see publication for more information)

text: E. coli DH10B

characteristic: lab-host

text: seed

characteristic: organ

text: Zea mays

text: SalI

text: NotI

text: hermaphrodite

text: endosperm

text: Maize Endosperm cDNA Library

text: plasmid


status: PUBLIC

text: North Carolina State University

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Cotton lint fiber

tag: attribute

characteristic: cell type

text: Deltapine 90

text: A suppressive subtraction hybridization (SSH) cDNA library was prepared with the PCR-select cDNA subtraction kit (BD Biosciences, ClonTech) using RNA from 20 DPA fiber as the tester and RNA from 6 DPA fiber as the driver

characteristic: description

text: Secondary wall stage, 20 days post anthesis (DPA)

characteristic: dev-stage

text: DH5 alpha

text: Gossypium hirsutum

characteristic: organism

text: G.h.fbr-sw

characteristic: title

text: plasmid

characteristic: vector


text: University of Helsinki

text: live

text: Generic

text: tissue_lib: P00011

text: 3-6 months, adult

text: Populus euphratica

text: leaf

characteristic: tissue-type

text: Populus euphratica leaf 3-6 months, adult


characteristic: External Id

text: Sri Krishnadevaraya University

text: live

text: Generic

text: VZM 1

text: Horse gram plants of 21-day-old were subjected to water stress (20%F.C) by withholding water for 48 hours and RNA isolated from leaf tissues. Subtracted cDNA library was made using PCR-based subtractive cloning method.

text: Three week old plant

text: Escherichia coli

text: Leaf

text: Macrotyloma uniflorum

text: Hermophrodite

characteristic: sex

text: Leaf

text: Horsegram stress induced cDNA library

text: pGEMT


text: Genome Research Center

text: live

text: Generic

text: suspension cultured cells

characteristic: cell-line

text: Whit Star

characteristic: cultivar

text: RNA was extracted from exponential stage of suspension cultured cells. cDNA library was constructed with Uni-ZAP XR vector using ZAP cDNA synthesis kit (STRATAGENE, USA)

text: exponential stage

characteristic: lab-host

text: Ipomoea batatas

text: EcoRI

text: XhoI

text: hermaphrodite

text: Plasmid

text: pBluescript (SK)


text: Biomedical Research Institute

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: Bge cell-line

text: B.glabrata (M-line strain) embryonic cell line Bge was cultured in complete Bge medium containing 10% FBS. Total RNA was isolated from the Bge cells and first strand cDNA synthesized using an oligo-dT primer-linker (XhoI). Second strand synthesis was followed by the ligation of EcoRI adaptors. Following digestion with XhoI, the completed, directional cDNA was cloned into Uni-ZAP XR Phagemid vector by Stratgene.

text: Biomphalaria glabrata

text: EcoRI

text: XhoI

text: hermaphrodite

text: M-line

characteristic: strain

text: Biomphalaria glabrata (Bge)cell line Library in Lambda ZAP

text: phagemid

text: pBluescript SK-


text: The Chinese University of Hong Kong

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Vegetative mycelium strain L54 was cultivated in Potato Dextrose medium. mRNA from mycelium was extracted and subtracted with parental monokaryotic mycelials A and B. The subsequently synthesized cDNA library was cloned into pTriplEx2 Vector (CloneTech).

text: Mycelium

text: Lentinula edodes

text: Sfi

text: Sfi

text: Dikaryon

text: Mycelial expressed cDNA library

text: Phage

text: pTriplEx2 Vector (CloneTech)


text: ESALQ - University of Sao Paulo

text: live

text: Generic

characteristic: NCBI submission package

text: This cDNA library was constructed with the SuperScript Plasmid System with Gateway Technology kit (Invitrogen), following manufacture's protocols. Plasmid DNA was purified using a modified alkaline lysis method. Sequencing reactions were conducted using the DYEnamic Cycle Sequencing Kit for MegaBACE (Amersham biosciences) according to the manufacturer's recommendations. Clones were sequenced by the 5' end with T7 primer. Sequencing reactions were analyzed on MegaBACE1000 DNA Sequencer (Amersham biosciences). The quality and clustering of the ESTs were analyzed using the softwares Phred/Cap3. Only EST sequences with Phred quality greater than 20 and at least 150 bp were considered for clustering.

characteristic: description

text: 1 and 21 days old

text: DH5 alpha

text: Gallus gallus

text: NotI

text: SalI

text: breast muscle

text: chicken breast muscle - CB1

text: Plasmid


text: ESALQ - University of Sao Paulo

text: live

text: Generic

text: embryos with 9 and 17 days old

characteristic: dev-stage

text: DH5 alpha

text: Gallus gallus

characteristic: organism

text: NotI

text: SalI

text: breast muscle

text: embryo breast muscle - EB1

text: Plasmid


text: ESALQ - University of Sao Paulo

text: live

text: Generic

text: embryos with 9 and 17 days old

text: DH5 alpha

text: Gallus gallus

text: NotI

text: SalI

text: breast muscle

text: embryo breast muscle - EB2

characteristic: title

text: Plasmid

characteristic: vector


characteristic: External Id

text: University of Texas at El Paso

text: live

text: Generic

text: juvenile

text: Fundulus heteroclitus

text: Fundulus heteroclitus juvenile


text: National Cancer Institute / NIH

text: live

text: Generic

text: RNA obtained from testis tissue of 8 wk old animal. Tissues were snap-frozen and kept at -80C before RNA extraction and purification (Tri-reagent method). cDNA was primed using oligo-dT primer: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3' and cloned into the EcoRV/NotI sites of pExpress-1. Size-selection >1.25kb resulted in an average insert size of 1.7 kb. This a primary library (normalized library is NIH_MGC_247) and was constructed by Open Biosystems. Note: this is a NIH_MGC library

text: DH10B TonA

text: liver

characteristic: organ

text: Rattus norvegicus

text: EcoRV

text: NotI

text: plasmid


text: National Cancer Institute / NIH

text: live

text: Generic

text: RNA obtained from testis tissue of 8 wk old animal. Tissues were snap-frozen and kept at -80C before RNA extraction and purification (Tri-reagent method). cDNA was primed using oligo-dT primer: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3' and cloned into the EcoRV/NotI sites of pExpress-1. Size-selection >1.25kb resulted in an average insert size of 1.7 kb. This is a primary library (normalized library is NIH_MGC_249) and was constructed by Open Biosytems. Note: this is a NIH_MGC library

text: DH10B TonA

text: spleen

text: Rattus norvegicus

text: EcoRV

text: NotI

text: plasmid


text: National Cancer Institute / NIH

text: live

text: Generic

text: RNA obtained from testis tissue of 8 wk old animal. Tissues were snap-frozen and kept at -80C before RNA extraction and purification (Tri-reagent method). cDNA was primed using oligo-dT primer: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3' and cloned into the EcoRV/NotI sites of pExpress-1. Size-selection >1.25kb resulted in an average insert size of 1.4 kb. This primary library is normalized ( primary library is NIH_MGC_248) and was constructed by Express Genomics (Frederick, MD). Note: this is a NIH_MGC library

text: DH10B TonA

text: spleen

text: Rattus norvegicus

text: EcoRV

text: NotI

text: plasmid


text: National Cancer Institute / NIH

text: live

text: Generic

text: RNA obtained from testis tissue of 8 wk old animal. Tissues were snap-frozen and kept at -80C before RNA extraction and purification (Tri-reagent method). cDNA was primed using oligo-dT primer: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3' and cloned into the EcoRV/NotI sites of pExpress-1. Size-selection >1.25kb resulted in an average insert size of 1.6 kb. This primary library is normalized (non-normalized primary library is NIH_MGC_246) and was constructed by Open Biosystems. Note: this is a NIH_MGC library

text: DH10B TonA

text: liver

text: Rattus norvegicus

text: EcoRV

text: NotI

text: plasmid


text: Universite di Perugia

text: live

text: Generic

text: Peripheral blood mononuclear cells

characteristic: cell type

text: This EST was obtained with the cDNA-AFLP method (Bachem et al., Plant Journal 9:745-753, 1996). Total RNA was extracted from two horses during a 160 km endurance ride. Blood samples were taken by jugular vein before ride and 0, 24 and 48 h after the competition. The restriction endonucleases EcoRI and MseI were used. Bands that resulted differentially expressed during the considered times were isolated from the polyacrilamide gel, cloned in a plasmid vector (Invitrogen PCR4 TOPO) and sequenced.

text: E.coli

text: Equus caballus

text: Blood

characteristic: tissue-type

text: Exercised Horse library

text: Plasmid

text: Invitrogen PCR4 TOPO Vector


text: The Samuel Roberts Noble Foundation

text: live

text: Generic

text: Glandular-haired alfalfa plants were established in a Conviron growth chamber (16-h days, full lights, 24oC set point) in 40 one gallon pots. Plants were grown in Metromix 350 and fertilized with MiracleGro as needed. They were cut back closely to encourage the emergence of vigorous shoots. Trichomes were isolated from stems, approximately 8-12 inches long, clipped from plants 2-3 inches above the crown. With minimal handling of the stem, the apical bud, leaves and nodes were discarded, and the trichomes isolated from the internode segments.

text: Trichomes were removed from internodes of 8-12 inch tall stems

text: Medicago sativa

text: Glandular trichomes isolated from stem

text: Glandular trichomes

text: Plasmid

text: pDNR-LIB


text: Seoul National University

text: live

text: Generic

text: mixed population

text: E. coli TOP10

characteristic: lab-host

text: Candida albicans

text: EcoRI

text: EcoRI

text: ATCC 10231

text: mRNA from farnesoic acid-responsive gene

text: plasmid

text: pCR2.1 vector


text: Institute of Surgery Research, Third Military Medical University

characteristic: INSDC center name

text: live

text: Generic

text: Rattus norvegicus

text: Rattus norvegicus library (Xie Z)


text: New Mexico State University

characteristic: INSDC first public

text: live

text: Generic

text: drought-stressed

text: leaf

text: Dactylis glomerata

text: Xho 1

text: Dactylis glomerata cDNA

text: Phage

text: Uni-ZAP XR


text: Institute of Marine Research

text: live

text: Generic

text: Library constructed by Frederick William Goetz, Great Lakes WATER Institute, University of Wisconsin Milwaukee, 600 E. Greenfield Ave., Milwaukee, WI, 53204

text: oocyte maturation and ovulation

text: ovary

text: Gadus morhua

text: Cod ovary Zap Express library

text: pBK-CMV


text: The University of Alabama at Birmingham

text: live

text: Generic

text: lymphocyte

text: lymphocyte mRNA ESTs from unstimulated larvae. All are from arrayed colonies from a directionally cloned cDNA library in Lambda ZAP Express (Stratagene). All are single pass 5' sequences.

text: unstimulated larvae

text: Petromyzon marinus

text: Sea lamprey LyEST

text: Lambda ZAP Express


text: The University of Alabama at Birmingham

text: live

text: Generic

text: lymphocyte

text: lymphocyte mRNA ESTs from PCR subtracted cDNA libraries of immune stimulated larvae. All are single pass 5' or 3' sequences randomly cloned in pGEM-T Easy (Promega).

text: immune stimulated larvae

text: Petromyzon marinus

text: Sea lamprey AcLy

text: pGEM-T Easy


text: The University of Alabama at Birmingham

text: live

text: Generic

text: myeloid

text: myeloid cells mRNA PCR subtracted cDNA libraries of immune stimulated larvae. All are single pass 5' or 3' sequences randomly cloned in pGEM-T Easy (Promega).

text: immune stimulated larvae

text: Petromyzon marinus

text: Sea lamprey AcMy

text: pGEM-T Easy


text: University of Bristol

text: live

text: Generic

text: Immature whole capitulum buds harvested at between 1-4mm diameter from multiple wild ecotypes were pooled, RNA extracted and mRNA purified. cDNA was prepared using the Stratagene pBlueScript II XR cDNA library construction kit and directionally cloned into pBlueScript II SK+ vector. 1152 clones were randomly picked based on blue/white selection with X-galactose. Clones were grown in 96-well microtitre plates containing terrific broth and selective antibiotic and are maintained as glycerol stocks in E. coli DH10B host strain.

text: 1-4mm diameter

text: E. coli DH10B

text: Senecio aethnensis

text: EcoRI

text: XhoI

text: Capitulum bud

text: Plasmid

text: pBlueScript II SK+


text: University of Bristol

text: live

text: Generic

text: Mature, opened, flower buds were harvested at multiple stages between flower opening and anthesis from a variety of wild ecotypes. Bracts were removed via crude dissection and tissue was pooled prior to RNA extraction and mRNA purification. cDNA was prepared using the Stratagene pBlueScript II XR cDNA library construction kit and directionally cloned into pBlueScript II SK+ vector. 1152 clones were randomly picked based on blue/white selection with X-galactose. Clones were grown in 96-well microtitre plates containing terrific broth and selective antibiotic and are maintained as glycerol stocks in E. coli DH10B host strain.

text: 1-4 days pre-anthesis

text: E. coli DH10B

text: Senecio aethnensis

text: EcoRI

text: XhoI

text: Flower and flower bud minus bracts

text: Plasmid

text: pBlueScript II SK+


text: University of Bristol

text: live

text: Generic

text: Immature whole capitulum buds harvested at between 1-4mm diameter from multiple wild ecotypes were pooled, RNA extracted and mRNA purified. cDNA was prepared using the Stratagene pBlueScript II XR cDNA library construction kit and directionally cloned into pBlueScript II SK+ vector. 1152 clones were randomly picked based on blue/white selection with X-galactose. Clones were grown in 96-well microtitre plates containing terrific broth and selective antibiotic and are maintained as glycerol stocks in E. coli DH10B host sain.

text: 1-4mm diameter

text: E. coli DH10B

text: Senecio cambrensis

text: EcoRI

text: XhoI

text: Capitulum bud

text: Plasmid

text: pBlueScript II SK+


text: University of Bristol

text: live

text: Generic

text: 1-4 days pre-anthesis

text: E. coli DH10B

text: Senecio cambrensis

text: EcoRI

text: XhoI

text: Flower and flower bud minus bracts

text: Plasmid

text: pBlueScript II SK+


text: University of Bristol

text: live

text: Generic

text: 1-4mm diameter

text: E. coli DH10B

text: Senecio squalidus subsp. squalidus

text: EcoRI

text: XhoI

text: Capitulum bud

text: Plasmid

text: pBlueScript II SK+


text: University of Bristol

text: live

text: Generic

text: 1-4 days pre-anthesis

text: E. coli DH10B

text: Senecio squalidus subsp. squalidus

text: EcoRI

text: XhoI

text: Flower and flower bud minus bracts

text: Plasmid

text: pBlueScript II SK+


text: University of Bristol

text: live

text: Generic

text: 1-4mm diameter

text: E. coli DH10B

text: Senecio vulgaris subsp. vulgaris

text: EcoRI

text: XhoI

text: Capitulum bud

text: Plasmid

text: pBlueScript II SK+


text: University of Bristol

text: live

text: Generic

text: 1-4 days pre-anthesis

text: E. coli DH10B

text: Senecio vulgaris subsp. vulgaris

text: EcoRI

text: XhoI

text: Flower and flower bud minus bracts

text: Plasmid

text: pBlueScript II SK+


text: University of Bristol

text: live

text: Generic

text: 1-4mm diameter

text: E. coli DH10B

text: Senecio chrysanthemifolius

text: EcoRI

text: XhoI

text: Capitulum bud

text: Plasmid

text: pBlueScript II SK+


text: University of Bristol

text: live

text: Generic

text: 1-4 days pre-anthesis

text: E. coli DH10B

text: Senecio chrysanthemifolius

text: EcoRI

text: XhoI

text: Flower and flower bud minus bracts

text: Plasmid

text: pBlueScript II SK+


text: Dairy and Swine Research and Development Center, Agriculture and Agri-Food Canada.

text: live

text: Generic

text: Library made from Suppressive Subtractive Hybridization from day-15 Gestating sows.

text: Sus scrofa

text: Female

characteristic: sex

text: Whole embryonic tissue

text: Pig embryonic cDNA library

text: pT-Adv vector


text: University of Hohenheim, Institute of Phytomedicine

characteristic: INSDC last update

text: live

text: Generic

text: cDNA-AFLP technique. RNA was extracted from final instar caterpillars and double-stranded cDNA was synthesized using the SMART PCR cDNA Synthesis Kit. The cDNA was digested with MseI, adapters were ligated to the termini, and two rounds of PCR were performed. On the first round the cDNAs were amplified with primers specific for the adapters. The products of this first round were amplified with primers based on the adapters containing one to three additional bases at their 3' ends.

text: final larval instar

text: Ephestia kuehniella


text: Korea University, College of Medicine

text: live

characteristic: INSDC status

text: Generic

text: Ordered Differential Display PCR product

text: adult

text: E. coli DH5_alpha

text: Rattus norvegicus

text: Sprague-Dawley rat

characteristic: strain

text: brain

text: Rattus norvegicus EST Library (Brain) stage adult

text: pGEM-T Easy


text: Korea University, College of Medicine

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: E. coli DH5_alpha

text: Rattus norvegicus

text: Sprague-Dawley rat

text: brain

text: Rattus norvegicus EST Library (Brain) stage E12

text: pGEM-T Easy


text: Korea University, College of Medicine

text: live

text: Generic

characteristic: description

characteristic: dev-stage

text: E. coli DH5_alpha

text: Rattus norvegicus

text: Sprague-Dawley rat

text: brain

text: Rattus norvegicus EST Library (Brain) stage E14

text: pGEM-T Easy


text: Korea University, College of Medicine

text: live

text: Generic

text: E. coli DH5_alpha

text: Rattus norvegicus

characteristic: organism

text: Sprague-Dawley rat

text: brain

text: Rattus norvegicus EST Library (Brain) stage E16

characteristic: title

text: pGEM-T Easy


characteristic: External Id

text: Korea University, College of Medicine

text: live

text: Generic

text: E. coli DH5_alpha

text: Rattus norvegicus

text: Sprague-Dawley rat

text: brain

text: Rattus norvegicus EST Library (Brain) stage E18

text: pGEM-T Easy

characteristic: vector


text: Korea University, College of Medicine

text: live

text: Generic

text: E. coli DH5_alpha

text: Rattus norvegicus

text: Sprague-Dawley rat

text: brain

text: Rattus norvegicus EST Library (Brain) stage E20

text: pGEM-T Easy


text: Korea University, College of Medicine

text: live

text: Generic

text: E. coli DH5_alpha

text: Rattus norvegicus

text: Sprague-Dawley rat

text: brain

text: Rattus norvegicus EST Library (Brain) stage P3

text: pGEM-T Easy


text: Korea University, College of Medicine

text: live

text: Generic

text: E. coli DH5_alpha

text: Rattus norvegicus

text: Sprague-Dawley rat

text: brain

text: Rattus norvegicus EST Library (Brain) stage P12

text: pGEM-T Easy


text: Korea University, College of Medicine

text: live

text: Generic

text: E. coli DH5_alpha

text: Rattus norvegicus

text: Sprague-Dawley rat

text: brain

text: Rattus norvegicus EST Library (Brain) stage P7

text: pGEM-T Easy


text: Korea University, College of Medicine

text: live

text: Generic

text: E. coli DH5_alpha

text: Rattus norvegicus

text: Sprague-Dawley rat

text: brain

text: Rattus norvegicus EST Library (Brain) stage P18

text: pGEM-T Easy


text: Korea University, College of Medicine

text: live

text: Generic

text: E. coli DH5_alpha

text: Rattus norvegicus

text: Sprague-Dawley rat

text: brain

text: Rattus norvegicus EST Library (Brain) stage P0

text: pGEM-T Easy


text: Korea University, College of Medicine

text: live

text: Generic

text: E. coli DH5_alpha

text: Rattus norvegicus

text: Sprague-Dawley rat

text: brain

text: Rattus norvegicus EST Library (Brain) stage P25

text: pGEM-T Easy


text: Korea University, College of Medicine

text: live

text: Generic

text: E. coli DH5_alpha

text: Rattus norvegicus

text: Sprague-Dawley rat

text: brain

text: Rattus norvegicus EST Library (Brain) stage P8

text: pGEM-T Easy


text: Korea University, College of Medicine

text: live

text: Generic

text: E. coli DH5_alpha

text: Rattus norvegicus

text: Sprague-Dawley rat

text: brain

text: Rattus norvegicus EST Library (Brain) stage P15

text: pGEM-T Easy


text: Richard Stockton College

text: live

text: Generic

text: Differentially expressed clones from reciprocal SSH libraries comparing fish exposed to fuel oil No. 6 with controls. OD-# indicates downregulation and OU-# upregulation with treatment.

text: juvenile young of the year

text: Liver

characteristic: organ

text: Pseudopleuronectes americanus

text: Winter Flounder Oil treated SSH

text: Plasmid

text: pGEM-T


text: Chulalongkorn University

text: live

text: Generic

text: The cDNA library was generated as part of Penaeus monodon EST project. Total RNA was isolated from hemocyte of the V. harveyi infected Penaeus monodon. The mRNA was further purified, reverse transcribed and cloned to Lambda Zip LOX.

text: Penaeus monodon

text: Not I

text: Sal I

text: Hemocyte

characteristic: tissue-type

text: Hemocyte - Vibrio harveyi infected library

text: Phage

text: Lambda Zip LOX


text: IB-Universidade Estadual de Campinas

text: live

text: Generic

text: The subtractive libraries were performed according cDNA representational difference analysis methodology.

text: Adult_newly emerged

text: Whole body

text: Melipona quadrifasciata anthidioides

text: Female

text: Subtractive cDNA libraries from queen and worker castes of Melipona quadrifasciata

text: Plasmid


text: Auburn University

text: live

text: Generic

text: One year old 'Owari' plants were grown for 5 weeks in a gradual cold acclimation temperature regime as follows: 32oC day/21_C night for 14 days; 27oC day/16oC night for 7 days; 24oC day/13oC night for 7 days and 18oC day/7oC night for 7 days. RNA was isolated from leaves collected at the end of second week and fifth week according to the RiboPure kit protocol (Ambion, TX). cDNA was synthesized using RETROscriptTM (Ambion, TX) according to the manufacturer's instructions, and digested using the MseI/EcoRI enzyme combination. AFLP analysis was conducted according to the methods of Bachem et al.(1996). DNA fragments were visualized by silver staining according to the Silver SequenceTM DNA Sequencing System Technical Manual (Promega, WI).

text: Escherichia coli DH5 cells

characteristic: lab-host

text: Citrus unshiu

text: Citrus unshiu cold acclimation with cDNA-AFLP

text: pGEM-T Easy vector system (Promega, WI)


text: Illumigen Biosciences Inc.

characteristic: INSDC center name

text: live

text: Generic

text: Created from CloneMiner cDNA Library Construction kit (catalog #18249-029)

text: adult

text: Electromax DH10B

text: duodenum

text: Macaca mulatta

text: BsrG I

text: BsrG I

text: male

text: Indian

text: lambda

text: pDONR 222


text: Illumigen Biosciences Inc.

characteristic: INSDC first public

text: live

text: Generic

text: adult

text: Electromax DH10B

text: ileum

text: Macaca mulatta

text: BsrG I

text: BsrG I

text: male

text: Indian

text: lambda

text: pDONR 222


text: Illumigen Biosciences Inc.

text: live

text: Generic

text: adult

text: Electromax DH10B

text: jejunum

text: Macaca mulatta

text: BsrG I

text: BsrG I

text: female

characteristic: sex

text: Indian

text: lambda

text: pDONR 222


text: Illumigen Biosciences Inc.

characteristic: INSDC last update

text: live

text: Generic

text: adult

text: Electromax DH10B

text: liver

text: Macaca mulatta

text: BsrG I

text: BsrG I

text: female

text: Indian

text: lambda

text: pDONR 222


text: Illumigen Biosciences Inc.

text: live

characteristic: INSDC status

text: Generic

text: adult

text: Electromax DH10B

text: ovary

text: Macaca mulatta

text: BsrG I

text: BsrG I

text: female

text: Indian

text: lambda

text: pDONR 222


text: Illumigen Biosciences Inc.

text: live

text: Generic

text: PBMC

characteristic: cell type

text: adult

text: Electromax DH10B

text: Macaca mulatta

text: BsrG I

text: BsrG I

text: male

text: Indian

characteristic: strain

text: blood

text: lambda

text: pDONR 222


text: Illumigen Biosciences Inc.

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Created from Stratagene ZAP-cDNA Synthesis kit (catalog #200400) and ZAP-CDNA Gigapack III Gold Cloning Kit (Catalog #200450)

text: newborn infant

text: E. coli SOLR

text: placenta

text: Macaca mulatta

text: EcoR I

text: Xho I

text: female

text: Indian

text: pBluescript SK (+/-) phagemid

text: Uni-ZAP XR


text: Illumigen Biosciences Inc.

text: live

text: Generic

characteristic: description

text: juvenile

characteristic: dev-stage

text: Electromax DH10B

text: Macaca mulatta

text: BsrG I

text: BsrG I

text: male

text: Indian

text: testis

text: lambda

text: pDONR 222


text: Illumigen Biosciences Inc.

text: live

text: Generic

text: Electromax DH10B

text: liver

text: Macaca nemestrina

characteristic: organism

text: BsrG I

text: BsrG I

text: male

text: Indian

characteristic: title

text: lambda

text: pDONR 222


characteristic: External Id

text: University of New Mexico

text: live

text: Generic

text: mRNA, pooled from 20 M-line B. glabrata snails, exposed to Schistosoma mansoni (12 hours), was used as template for RT-PCR with random primers (18nt) to generate ORESTES(ORF-ESTs). Resulting cDNAs were shotgun cloned (pCR2.1 TOPO TA, Invitrogen), and sequenced using primer M13(-20). cammo = C Adema M-line Mansoni Orestes. Random primers were removed from sequence as presented.

text: adult

text: Biomphalaria glabrata

text: hermaphrodite

text: M-line

text: whole body

text: cammo

text: Plasmid

characteristic: vector


text: Universite Pierre et Marie Curie

text: live

text: Generic

text: RT-PCR using degenerate CYP4 and CYP6 primers. Cloning in PGEM-T easy.

text: 4th instar larvae

text: Aedes aegypti

text: male and female

text: CYPs cDNA from Aedes aegypti larvae

text: PGEMT-easy


text: Washington State University

text: live

text: Generic

text: Gametocysts of Gregarina niphandrodes were obtained from Tenebrio molitor. The cDNA library was constructed at Washington Univ. cDNA was synthesized from poly(A)+ mRNA using the template-switching PCR method (SMART cDNA Kit, BD Biosciences). First strand cDNA was reverse transcribed using the CDS III/3' primer and a 5' template switch primer (Smart IV primer). The product of the first strand synthesis was PCR amplified using the same primer set and the fragments were digested with SfiI. The fragments were size selected, ligated into a modified pBluescript vector (obtained from Michael White, Montana State University) containing directional SfiI sites, and electroporated into ElectroTen Blue cells. Vector: SfiI sites were added to the multiple cloning region of pBluescript SK+ between the BamHI/EcoRI sites. The modified polylinker has the following sequence: 5'GAATTCGGCCATTACGGCC (G)n-- insert-- GGCCGCCTCGGCCCACGGATCC3'where n=3-4 G nucleotides.

text: gametocyst

text: GC10 Competent Cells(PGC

text: Gregarina niphandrodes

text: Gregarina niphandrodes gametocyst cDNA library

text: modified pBluescript (pBS SK+)


text: USDA-ARS

text: live

text: Generic

text: A high quality EST with at least 200 contiguous bases at Trace Tuner score of 20 or better. Construction by L Hunnicutt and PM DANG,USDA,ARS, U.S. Horticultural Res. Lab, Ft.Pierce, FL, USA.

text: Adult

text: Homalodisca vitripennis

text: EcoRI

text: XhoI

text: Mixed population

text: Adult whole body

characteristic: tissue-type

text: Adult Glassy-winged Sharpshooter

text: pBluescript II SK+


text: Station Biologique de Roscoff

text: live

text: Generic

text: protoplasts

text: Chondrus crispus

text: Eco R1

text: Chondrus crispus protoplasts Zap Express Library

text: pbK-CMV


text: Station Biologique de Roscoff

text: live

text: Generic

text: thallus

text: Chondrus crispus

text: Eco R1

text: Chondrus crispus thallus Zap Express Library

text: pBluescript


text: UMR 7100 - IFR 85 - ESBS -ULP

text: live

text: Generic

text: Strisselspalt

characteristic: cultivar

text: The subtractive hybridization protocol from BD Biosciences Clontech (Palo Alto, CA) has been used to generate this specific cDNA library. Tester cDNA was prepared from mature female hop flowers (cones) and driver cDNA was from female hop flower buds. The resulting cDNAs were cloned into the Invitrogen (Carlsbad, CA) pCR4-Topo vector.

text: Mature

text: E.coli TOP10 F'

characteristic: lab-host

text: Flower

characteristic: organ

text: Humulus lupulus

text: Female

text: HlF HSS cDNA

text: Plasmid


text: Wageningen University

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: Bintje

text: Total RNA was isolated from developing axillary buds of potato nodal stem cuttings cultured on medium for the introduction of tuber formation. Tissue samples were taken of stages corresponding to growing stolons and the early stages of tuber formation.

text: days 1 to 10

text: Solanum tuberosum

text: axillary buds of stem explants, swelling stolons, in vitro tubers

text: Potato in vitro tuberization cDNA-AFLP fragments


text: University of Paisley

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Golf

text: RT-PCR

text: Hordeum vulgare

text: leaf

text: Hordeum vulgare cv. Golf leaf

text: Plasmid

text: pGEM_T Easy


text: Washington University School of Medicine

text: live

text: Generic

text: GoldRush

text: Total RNA was extracted separately from each stage [dormant terminal bud, dormant lateral bud, active lateral bud], using the 'pine tree' method. Poly(A)+mRNA was isolated twice from total RNA from each stage using the Oligotex Direct mRNA kit (Qiagen). mRNA was reverse transcribed into double stranded cDNA using a modified oligo18(dT) primer with an identifying tag sequence (see table below). cDNAs from different stages were pooled in equal amounts before adaptor ligation. Tag identification when sequencing from 5' end: Stage 1 (dormant terminal bud) insert 18(A)TCGTG; Stage 2 (dormant lateral bud) insert 18(A)TGCTG; Stage 3 (active lateral bud) insert 18(A)TCGGT; Tag identification when sequencing from 3' end: Stage 1 (dormant terminal bud) CACGA18(T) insert; Stage 2 (dormant lateral bud) CAGCA18(T) insert; Stage 3 (active lateral bud) ACCGA18(T) insert; Double stranded cDNAs were size selected (more than 450 bp), adaptored with EcoRI adapters at both ends and then digested with NotI. The cDNAs were then directionally cloned into EcoR1-NotI digested pBS II SK(+) phagemid vector(Stratagene). Identification of adaptors and tags in 5'-end sequenced clones: (Vector)...TAAGCTT(End Vector)(Start EcoR1_adaptor)GATATCGAATTCCATTGTGTTGGG (End EcoR1_adaptor)(Start Insert)....AAAAAAAAAAAAAAAAAA(End Insert) (Start Tag)TGCGA(End Tag)(Start NotI_site/Vector)GCGGCCGCCACCGCGG... The total number of white colony forming units (cfu) in the primary library before amplification was 4x10^5 cfu (colony forming units). The background of empty clones was less than 10%. Inserts ranged from 0.5 kb to 4 kb, as determined by PCR. Purified plasmid DNA from the primary library was converted to single-stranded circles and used as a template for PCR amplification using the T7 and T3 priming sites flanking the cloned cDNA inserts. The purified PCR products, representing the entire cloned cDNA population, were used as a driver for normalization. Hybridization between the single-stranded library and the PCR products was carried out for 44 h at 30C. Unhybridized single-stranded DNA circles were separated from hybridized DNA rendered partially double-stranded and electroporated into DH10B cells to generate the normalized library. The total number of clones with insert was 1x10^6 cfu. Background of empty clones was less than 2%.

text: DH10B ampicillin resistant

text: Malus domestica

text: NotI

text: Bud

text: Mddb

text: Phagemid (ampicillin resistant)

text: pBluescript II SK (+)


text: Universidade Catolica de Brasilia

text: live

text: Generic

text: First strand was synthetized with Superscript II reverse transcriptase and an oligo-dT-Not primer (5'-CCTGCGGCCGCT(18)-3'). Synthesis of the cDNA second strand was performed using the exo-Klenow fragment of DNA polymerase with a spliced leader (SL) primer (5'-GATACAGTTTCTGTA-3')

text: Plant promastigote

text: Lycopersicon esculentum (tomato)

text: Phytomonas serpens

text: EcoRI

text: NotI

characteristic: strain

text: fruit

text: Phytomonas serpens plant-stage cDNA library

text: Phagemid

text: pBluescript SK+


text: Kansas State University

text: live

text: Generic

characteristic: NCBI submission model

text: Felis catus

text: Uterus

text: Feline cDNA, uterus library

text: Phage

text: Uni-ZAP(r) XR


text: Guru Nanak Dev University

text: live

text: Generic

characteristic: NCBI submission package

text: Sorghum bicolor

text: Sorghum bicolor cv. ICSV-272


text: Institute of Medical Science, University of Tokyo

text: live

text: Generic

characteristic: cell-line

text: Homo sapiens

text: embryonal kidney

text: Sugano cDNA library, embryonal kidney


text: University of Hannover

text: live

text: Generic

text: Elstar

text: Total RNAs were isolated from the Malus domestica cultivars Remo (resistant against Venturia inaequalis) and Elstar (susceptible). The library was constructed using the PCR Select cDNA subtraction kit (Clontech, Palo Alto, CA, USA). Briefly, cDNA was obtained from total RNA using the SMART cDNA synthesis kit (Clontech, Palo Alto, CA, USA) by using an OligodT primer which allowed the amplification of the complete mRNA population contained in each sample. The cDNA populations of the tester (Elstar) and the driver (Remo), were digested with the restriction enzyme RsaI (Gibco, USA) to obtain short blunt-ended fragments. The tester pool was then divided in two populations, and the first one was ligated to the adaptor 1 and the second one to the adaptor 2R, provided with the kit. Each tester pool was hybridised separately with excess driver cDNA, and finally mixed together for a second subtractive hybridisation. The fragments differentially expressed in the tester were then amplified in two PCR reactions, according to the manufacturer's recommendations. The fragments obtained were cloned into the pGEM-T Vector (Promega, Mannheim, Germany), which were used for the transformation of the E. coli XL-1 blue (Stratagene, La Jolla, CA, USA).

characteristic: description

text: young leaves

characteristic: dev-stage

text: E.coli XL-1 blue

text: Malus domestica

text: leaves

text: Plasmid

text: pGemT, ampicillin resistant


text: University of Hannover

text: live

text: Generic

text: Remo

text: Total RNAs were isolated from the Malus domestica cultivars Remo (resistant against Venturia inaequalis) and Elstar (susceptible). The library was constructed using the PCR Select cDNA subtraction kit (Clontech, Palo Alto, CA, USA). Briefly, cDNA was obtained from total RNA using the SMART cDNA synthesis kit (Clontech, Palo Alto, CA, USA) by using an OligodT primer which allowed the amplification of the complete mRNA population contained in each sample. The cDNA populations of the tester (Remo) and the driver (Elstar), were digested with the restriction enzyme RsaI (Gibco, USA) to obtain short blunt-ended fragments. The tester pool was then divided in two populations, and the first one was ligated to the adaptor 1 and the second one to the adaptor 2R, provided with the kit. Each tester pool was hybridised separately with excess driver cDNA, and finally mixed together for a second subtractive hybridisation. The fragments differentially expressed in the tester were then amplified in two PCR reactions, according to the manufacturer's recommendations. The fragments obtained were cloned into the pGEM-T Vector (Promega, Mannheim, Germany), which were used for the transformation of the E. coli XL-1 blue (Stratagene, La Jolla, CA, USA).

text: young leaves

text: E.coli XL-1 blue

text: Malus domestica

characteristic: organism

text: leaves

characteristic: title

text: Plasmid

text: pGemT, ampicillin resistant


characteristic: External Id

text: University of Puerto Rico

text: live

text: Generic

text: The pupal body library was prepared from pupae with the developing wings removed. Pupae were mixed from several developmental stages between 2 and 5 days after pupation. Source material was derived from Gamboa, Republic of Panama

text: Pupa

text: Heliconius melpomene

text: Body

text: Heliconius melpomene pupal body EST library

characteristic: vector


text: live

text: Generic

text: Library constructed by: Daniel K. Howe and Michelle R. Yeargan Total RNA was isolated from culture-derived merozoites of Sarcocystis neurona strain SN4. cDNA was synthesized using the template-switching and long-distance PCR method (SMART cDNA library construction kit, BD Biosciences). The amplified cDNA fragments were digested with SfiI, size fractionated, and ligated into SfiI-digested pDNR-LIB vector.

text: merozoite

text: Sarcocystis neurona

text: GGCCGCCTCGGCC

text: GGCCATTACGGCC

text: Sarcocystis neurona merozoite UK cSN4 1 cDNA library

text: plasmid

text: pDNR-LIB


text: The University of Queensland

text: live

text: Generic

text: Smooth Cayenne

characteristic: cultivar

text: Mature green fruit

text: Ananas comosus

text: Fruit flesh

characteristic: tissue-type

text: Pineapple green mature fruit cDNA library


text: Centre for Environmental Stress & Adaptation Research

text: live

text: Generic

text: Plutella xylostella

text: Plutella xylostella EST Library Jena


text: University of Liverpool

text: live

text: Generic

text: SSH library of up and down regulated liver cDNAs between summer and winter animals

text: Adult

text: E.coli Electromax DH10B

characteristic: lab-host

text: Callospermophilus lateralis

text: EcorI GAATTC

text: EcorI GAATTC

text: Male & female

characteristic: sex

text: Liver

text: squirrel liver library 1

text: Plasmid

text: pGEMT-easy


text: University of Liverpool

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: Normalized and subtracted cDNA library prepared from testis of hibernating and summer animals

text: Adult

text: E.coli Electromax DH10B

text: Callospermophilus lateralis

text: SalI GTCGAG

text: BamHI GGATCC

text: Male & female

text: Testis

text: squirrel testis library 1

text: Plasmid

text: pFLC


text: University of Liverpool

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Normalized and subtracted cDNA library prepared from heart of hibernating and summer animals

text: Adult

text: E.coli Electromax DH10B

text: Callospermophilus lateralis

text: SalI GTCGAG

text: BamHI GGATCC

text: Male & female

text: Heart

text: squirrel heart library 1

text: Plasmid

text: pFLC


text: University of Liverpool

text: live

text: Generic

characteristic: NCBI submission model

text: Normalized and subtracted cDNA library prepared from embryos

text: embryonic

text: E.coli Electromax DH10B

text: Callospermophilus lateralis

text: SalI GTCGAG

text: BamHI GGATCC

text: male and female

text: embryo

text: squirrel embryo library 1

text: Plasmid

text: pFLC


text: University of Liverpool

text: live

text: Generic

characteristic: NCBI submission package

text: SSH library of up and down regulated cDNAs between summer and winter animals

text: Adult

text: E.coli Electromax DH10B

text: Callospermophilus lateralis

text: EcorI GAATTC

text: EcorI GAATTC

text: Male & female

text: liver

text: squirrel liver library 2

text: Plasmid

text: pGEMT-easy


text: University of Liverpool

text: live

text: Generic

text: Normalized cDNA library prepared from liver of hibernating and summer animals

text: Adult

text: E.coli Electromax DH10B

text: Callospermophilus lateralis

text: Sfi1 GGCCATTACGGCC

text: Sfi1 GGCCGCCTCGGCC

text: Male & female

text: liver

text: squirrel liver library 3

text: Plasmid


text: University of Liverpool

text: live

text: Generic

text: Normalized cDNA library prepared from brain of hibernating and summer animals

text: Adult

text: E.coli Electromax DH10B

text: Callospermophilus lateralis

text: Sfi1 GGCCATTACGGCC

text: Sfi1 GGCCGCCTCGGCC

text: Male & female

text: brain

text: squirrel brain library 1

text: Plasmid


text: University of Liverpool

text: live

text: Generic

text: Adult

text: E.coli Electromax DH10B

text: Callospermophilus lateralis

text: EcorI GAATTC

text: EcorI GAATTC

text: Male & female

text: brain

text: squirrel brain library 2

text: Plasmid

text: pGEMT-easy


text: live

text: Generic

text: cDNA library was constructed from the leaves of Chiifu, using the ZAP-cDNA Synyhesis and cloning kit of Stratagene. Total RNA was isolated from non-treated leaves using TRIzol(Gibco, BRL) and subsequently poly-A+mRNA was extracted. First-strand cDNA was synthesized from poly(A) RNA(5') with MMLV-RT (Reverse transcriptase) at 37o for 1 hour. DNA polymeraseI and second-strand dNTP mixture was used in second-strand cDNA synthesis at 16o for 2.5 hours. EcoRI and XhoI adapters were added to phosphorylated cDNA ends. After size fractionating, cDNAs longer than 800bp were cloned into Uni-ZAP XR vector and packaged Gigapack III Gold (Stratagene) according to the manufacturer's specifications.

text: 1 months old plant

text: Brassica rapa subsp. pekinensis

text: Leaf

text: A leaf cDNA library of Chinese Cabbage, Brassica rapa


text: Universidad Nacional de Tucuman

text: live

text: Generic

text: Pajaro

text: leaves

characteristic: organ

text: Fragaria x ananassa

text: Strawberry leaves infected with Colletotrichum spp.


text: School of Agricultural Biotechnology, Seoul National University

text: live

text: Generic

text: The libraries were synthesized using the ZAP(r)-cDNA synthesis method (Stratagene). cDNA was prepared, size-fractionated and inserted into the Uni-ZAP XR vector using XhoI linker-primer and EcoRI adaptor. After in vivo excision with E. coli strain SOLR, cDNA libraries from brain contain each insert size range of 0.5~3kb (n=20), and 0.5~3.5kb (n=20).

characteristic: description

text: Gallus gallus

text: XhoI

text: EcoRI

text: Male

text: Korean Native chicken

characteristic: strain

text: Brain

text: Brain cDNA Library

text: phage

text: Uni-ZAP XR vector


text: live

text: Generic

text: The libraries were synthesized using the ZAP(r)-cDNA synthesis method (Stratagene). cDNA was prepared, size-fractionated and inserted into the Uni-ZAP XR vector using XhoI linker-primer and EcoRI adaptor. After in vivo excision with E. coli strain SOLR, cDNA libraries from spleen contain each insert size range of 0.5~3kb (n=20), and 0.5~3.5kb (n=20).

characteristic: dev-stage

text: Gallus gallus

characteristic: organism

text: XhoI

text: EcoRI

text: Male

text: Korean Native chicken

text: Spleen

text: Spleen cDNA Library

text: phage

text: Uni-ZAP XR vector


characteristic: External Id

text: live

text: Generic

text: The libraries were synthesized using the ZAP(r)-cDNA synthesis method (Stratagene). cDNA was prepared, size-fractionated and inserted into the Uni-ZAP XR vector using XhoI linker-primer and EcoRI adaptor. After in vivo excision with E. coli strain SOLR, cDNA libraries from testis contain each insert size range of 0.5~3kb (n=20), and 0.5~3.5kb (n=20).

text: Gallus gallus

text: XhoI

text: EcoRI

text: Male

text: Korean Native chicken

text: Testis

text: Testis cDNA Library

characteristic: title

text: phage

text: Uni-ZAP XR vector


text: Illumigen Biosciences Inc.

text: live

text: Generic

text: Created from CloneMiner cDNA Library Construction kit (catalog #18249-029)

text: adult

text: Electromax DH10B

text: liver

text: Macaca fascicularis

text: BsrG I

text: BsrG I

text: Indian

text: lambda

text: pDONR 222

characteristic: vector


text: Gyeongsang National University

text: live

text: Generic

text: Nokkwang

characteristic: cultivar

text: cDNA library was generated from red ripe fruit pericarp using lambda Zap II phage vector. In vivo excision was done with helper phage to generate subclone in pBluescript SK(-) vector.

text: Capsicum annuum

text: EcoRI

text: XhoI

text: red ripe fruit pericarp

characteristic: tissue-type

text: Plasmid

text: pBluescript SK(-)


text: National Cancer Institute / NIH

text: live

text: Generic

text: cDNA was primed using oligo-dT primer: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3' and cloned into the EcoRV/NotI sites of pExpress-1. Size-selection >0.5 kb resulted in an average insert size of 1.2 kb. This is a primarylibrary (normalized primary library is NIH_MGC_257) and was constructed by Express Genomics (Frederick, MD). Note: this is a NIH_MGC library

text: DH10B TonA

characteristic: lab-host

text: oocyte

text: Mus musculus

text: EcoRV

text: NotI

text: plasmid


text: National Cancer Institute / NIH

characteristic: INSDC center name

text: live

text: Generic

text: cDNA was primed using oligo-dT primer: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3' and cloned into the EcoRV/NotI sites of pExpress-1. Size-selection >0.5 kb resulted in an average insert size of 1.0kb. This is a normalized library (primary library is NIH_MGC_256) and was constructed by Express Genomics (Frederick, MD). Note: this is a NIH_MGC library

text: DH10B TonA

text: oocyte

text: Mus musculus

text: EcoRV

text: NotI

text: plasmid


text: Chulalongkorn University

characteristic: INSDC first public

text: live

text: Generic

text: The cDNA library was generated as part of Penaeus monodon EST project. Total RNA was isolated from hepatopancrease of Penaeus monodon. The mRNA was further purified, reverse transcribed and cloned to Uni-ZAP XR.

text: Penaeus monodon

text: EcoR I

text: Xho I

text: Hepatopancrease

text: Hepatopancrease library

text: Phage

text: Uni-ZAP XR


text: Chulalongkorn University

characteristic: INSDC last update

text: live

text: Generic

text: The cDNA library was generated as part of Penaeus monodon EST project. Total RNA was isolated from hepatopancrease of Penaeus monodon. The mRNA was further purified, reverse transcribed and cloned to Uni-ZAP XR. The cDNA library in the form of single-stranded was hybridized with cDNA inserts excised from a plasmid DNA preparation of library itself. The unbound single-stranded DNA was transformed into XL-Blue.

text: Penaeus monodon

text: EcoR I

text: Xho I

text: Hepatopancrease

text: Hepatopancrease-normalized

text: Phage

text: Uni-ZAP XR


text: Chulalongkorn University

text: live

characteristic: INSDC status

text: Generic

text: The cDNA library was generated as part of Penaeus monodon EST project. Total RNA was isolated from lymphoid organ of Penaeus monodon. The mRNA was further purified, reverse transcribed and cloned to Uni-ZAP XR.

text: Penaeus monodon

text: EcoR I

text: Xho I

text: Lymphoid organ

text: Lymphoid organ library

text: Phage

text: Uni-ZAP XR


text: Chulalongkorn University

text: live

text: Generic

characteristic: NCBI submission model

text: The cDNA library was generated as part of Penaeus monodon EST project. Total RNA was isolated from lymphoid organ of Penaeus monodon. The mRNA was further purified, reverse transcribed and cloned to Uni-ZAP XR. The cDNA library in the form of single-stranded was hybridized with cDNA inserts excised from a plasmid DNA preparation of library itself. The unbound single-stranded DNA was transformed into XL-Blue.

text: Penaeus monodon

text: EcoR I

text: Xho I

text: Lymphoid organ

text: Lymphoid organ-normalized

text: Phage

text: Uni-ZAP XR


text: Chulalongkorn University

text: live

text: Generic

characteristic: NCBI submission package

text: The cDNA library was generated as part of Penaeus monodon EST project. Total RNA was isolated from lymphoid organ of the V. harveyi infected Penaeus monodon. The mRNA was further purified, reverse transcribed and cloned to Uni-ZAP XR.

text: Penaeus monodon

text: EcoR I

text: Xho I

text: Lymphoid organ

text: Lymphoid organ - Vibrio harveyi infected library

text: Phage

text: Uni-ZAP XR


text: Max Planck Institute of Molecular Cell Biology and Genetics, Dresden

text: live

text: Generic

text: regenerating tail blastema

characteristic: cell type

text: Unnormalized cDNA plasmid library prepared by Invitrogen. Size fractionated mRNA was polydT primed and cloned into NotI-SalI site of pCMVSport6. Bacterial host is EMDH10B-TONA. Average insert size is 1.67 kB.

text: Ambystoma mexicanum

text: NotI

text: SalI

text: Tail Blastema

text: 6-Day Axolotl Tail Blastema (6DAxBL)

text: Plasmid


text: live

text: Generic

text: Includes Neural tube, notochord, somites

text: Unnormalized cDNA plasmid library prepared by Invitrogen. Size fractionated mRNA was polydT primed and cloned into NotI-SalI site of pCMVSport6. Bacterial host is EMDH10B-TONA. Average insert size is 1.5 kB.

characteristic: description

text: Stage 18-22

text: Ambystoma mexicanum

text: NotI

text: SalI

text: Neural Tube, Notochord, Somites

text: St18-22 Neural tube (NT)

text: Plasmid


text: Instituto Butantan

text: live

text: Generic

text: 5ug of mRNA from Thalassophrine nattereri venom glands were primed with oligo-(dT) and reverse transcribed to cDNA using Superscript Plasmid System for cDNA Synthesis and Cloning (Invitrogen). The cDNAs were selected by size (400-800 pb and up 800 pb) in agarose gel electrophoresis, linked to Eco RI adapters and directionally cloned in pGEM11Zf+ vector (Promega). ESTs were generated from random clones and grouped in unique sequences. The putative identification of each EST or cluster was obtained through Blast searches (e-value < e-05).

text: glands

characteristic: organ

text: Thalassophryne nattereri

text: Eco RI

text: Not I

text: venom glands

text: Thalassophryne nattereri library TNA

text: plasmid


text: Western Washington University

text: live

text: Generic

text: embryo, 0-24 hrs after egg laying

characteristic: dev-stage

text: Nasonia vitripennis

text: Nasonia PDNR Embryonic Library (emb2)

text: Plasmid

text: Clontech pDNR-Lib


text: University of Florida

text: live

text: Generic

text: 'Strawberry Festival'

text: Fragaria x ananassa 'Strawberry Festival' cultivar (octoploid), total RNA derived from whole plant vegetative tissues 24 h after treatment with salicylic acid (4 uM foliar spray and 1 uM root drench). For more details on library preparation and sequence analysis see http://www.hos.ufl.edu/shortcake and http://www.genome.clemson.edu/gdr To order clones from this library see http://www.arabidopsisthaliana.com/strawberry/clones.htm

text: E. coli

text: Fragaria x ananassa

characteristic: organism

text: EcoRI

text: XhoI

text: Whole plant treated 24 h with 1mM salicylic acid (spray), 4 mM soak

text: Fragaria x ananassa 'Strawberry Festival' cultivar 24h after treatment with salicylic acid

text: Plasmid

text: pBluescript


characteristic: External Id

text: CSIRO

text: live

text: Generic

text: Cabernet Sauvignon

text: A cDNA library from preveraison fruit, 1-2 weeks post anthesis, at stage 28 of the modified E-L system. Tissue collected from field grown plants. A description of the modified E-L system can be found in the paper by B. G. Coombe 'Adoption of a system for identifying grapevine growth stages' (1995) Aust. J. Grape and Wine Res. 1: 104-110.

text: 28 - modified E-L system

text: Fruit

text: Vitis vinifera

text: Hermaphrodite

characteristic: sex

text: CabSau Normalised Berry Fruit Set Stage 28 (PREn0028)

text: Plasmid

text: pZL


text: Institute of zoology, Chinese Academy of Sciences

text: live

text: Generic

text: fifth-instar

text: Locusta migratoria

text: whole-body

text: Locusta migratoria gregarious phase's whole-body cDNA library

characteristic: title


text: live

text: Generic

text: fifth-instar

text: Locusta migratoria

text: head

text: Locusta migratoria gregarious phase's head cDNA library


text: live

text: Generic

text: fifth-instar

text: Locusta migratoria

text: hind-legs

text: Locusta migratoria gregarious phase's hind-legs cDNA library


text: live

text: Generic

text: fifth-instar

text: Locusta migratoria

text: midgut

text: Locusta migratoria gregarious phase's midgut cDNA library


text: live

text: Generic

text: fifth-instar

text: Locusta migratoria

text: head

text: Locusta migratoria solitarious phase's head cDNA library


text: live

text: Generic

text: fifth-instar

text: Locusta migratoria

text: hind-legs

text: Locusta migratoria solitarious phase's hind-legs cDNA library


text: live

text: Generic

text: fifth-instar

text: Locusta migratoria

text: midgut

text: Locusta migratoria solitarious phase's midgut cDNA library


text: Animal Technology Institute Taiwan

text: live

text: Generic

text: Material with normal function was tested by Divisions of Biotechnology and Animal Resources, Animal Technology Institute Taiwan (ATIT). First strand cDNA was primed with a NotI-oligo(dT) primer and 2nd strand cDNA was synthesized by using nick translational replacement of the mRNA. Double-strand cDNA was added with SalI adapter, then digested with NotI and cloned into the NotI and SalI sites of pSPORT1 vector.

text: adult, l year of age

text: DH10B (Life Technologies)

text: testis

text: Sus scrofa

text: NotI

text: SalI

text: male

text: Duroc

characteristic: strain

text: Porcine testis cDNA library I

text: Plasmid

characteristic: vector


text: Sri Krishnadevaraya University

text: live

text: Generic

characteristic: cultivar

text: Groundnut plants of 21-day-old were subjected to water stress (20%F.C) by withholding water for 48 hours and RNA isolated from leaf tissues. Subtracted cDNA library was made using PCR-based subtractive cloning method.

text: Three week old plant

text: Escherichia coli

characteristic: lab-host

text: Leaf

text: Arachis hypogaea

text: EcoRI

text: EcoRI

text: Hermaphrodite

text: Leaf tissue

characteristic: tissue-type

text: Groundnut stress induced cDNA library


text: University of Edinburgh

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: mixed

text: Eurydice pulchra

text: EcoRI

text: XhoI

text: mixed

text: Eurydice pulchra CT library

text: plasmid

text: pBluescript SK-


text: Cardiff University

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Juvenile

text: Whole worm

text: Lumbricus rubellus

text: mixed

text: Whole worm

text: Juvenile Earthworm Library


text: The Natural History Museum

text: live

text: Generic

text: mRNA, extracted from schistosome-unexposed 3017 (resistant) snails was used as a template for RT-PCR with random primers to generate cDNA fragments. These were cloned and sequenced using M13F. Primer sequences were removed from sequence.

characteristic: description

text: Biomphalaria glabrata

text: Hermaphrodite

text: NHM 3017

text: Haemocytes

text: BgORESTES unexposed NHM 3017 Haemocytes 2

text: pGEM


text: The Natural History Museum

text: live

text: Generic

text: mRNA, extracted from schistosome-exposed 3017 (resistant) snails was used as a template for RT-PCR with random primers to generate cDNA fragments. These were cloned and sequenced using M13F. Primer sequences were removed from sequence.

text: Biomphalaria glabrata

text: Hermaphrodite

text: NHM 3017

text: Haemocytes

text: BgORESTES schistosome-exposed NHM 3017 Haemocytes 2

text: pGEM


text: The Natural History Museum

text: live

text: Generic

text: Ovotestis

characteristic: organ

text: Biomphalaria glabrata

text: Hermaphrodite

text: NHM 3017

text: BgORESTES unexposed NHM 3017 Ovotestis 2

text: pGEM


text: The Natural History Museum

text: live

text: Generic

text: Ovotestis

text: Biomphalaria glabrata

characteristic: organism

text: Hermaphrodite

text: NHM 3017

text: BgORESTES schistosome-exposed NHM 3017 Ovotestis 3

text: pGEM


characteristic: External Id

text: The Natural History Museum

text: live

text: Generic

text: Head/foot

text: Biomphalaria glabrata

text: Hermaphrodite

text: NHM 3017

text: BgORESTES unexposed NHM 3017 Head/foot 2

text: pGEM


text: The Natural History Museum

text: live

text: Generic

text: Biomphalaria glabrata

text: Hermaphrodite

characteristic: sex

text: NHM 3017

text: Head/foot

text: BgORESTES schistosome-exposed NHM 3017 Head/foot 3

text: pGEM


text: Washington University School of Medicine

text: live

text: Generic

text: The library was constructed by Irina Ronko and Dr. Makedonka Mitreva (mmitreva@watson.wustl.edu) at Washington University, St. Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis cDNA PCR products of size >400 nucleotides containing SL1 on the 5' end and oligo(dT) on the 3' end were non-directionally cloned into pCRII-TOPO(Invitrogen) following the Topo TA cloning protocol. Radopholus similis total RNA was provided by Dr. Godelieve Gheysen of Ghent University (lighe@gengenp.rug.ac.be).

text: Mixed

characteristic: dev-stage

text: Radopholus similis

text: 5': EcoRI

text: 3': EcoRI

text: Radopholus similis SL1 TOPO v1

characteristic: title

text: plasmid (kanamycin and ampicillin resistant)

text: pCRII-TOPO (Invitrogen)


text: live

text: Generic

text: The library was constructed by Irina Ronko and Dr. Makedonka Mitreva (mmitreva@watson.wustl.edu) at Washington University, St. Louis. Oligo(dT)-SL2c PCR based library. Pristionchus pacificus mixed stage cDNA PCR products of size >400 nucleotides containing SL2c (GGTTTATACCCAGTATCTCAAG) on the 5' end and oligo(dT) on the 3' end were non-directionally cloned into pCRII-TOPO (Invitrogen) following the Topo TA cloning protocol.

text: Mixed

text: Pristionchus pacificus

text: 5': EcoRI

text: 3': EcoRI

text: Pristionchus pacificus mixed stage SL2c TOPO v1

text: pCRII-TOPO (Invitrogen)

characteristic: vector


text: The Kansai Electric Power Co.

text: live

text: Generic

text: The cDNA clone was isolated from the cDNA library of Chlamydomonas W80 by a functional expression screening in E. coli cells. The principle of the screening method was based on the acquisition of cadmium-stress tolerance of the bacterial cells carrying the cDNA

text: Chlamydomonas sp. W80

text: Chlamydomonas sp. W80 cadmium-stress tolerance clones


text: Max-Planck-Institut fuer Molekulare Genetik

text: live

text: Generic

text: Random primed and directionally cloned in pSport1 vector using NotI (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC (T)15-3' and SalI 5'- TCGACCCACGCGTCCG-3' adapters (Gibco BRL)

text: adult brain

text: brain

text: Bos taurus

text: NotI

text: SalI

text: female

text: brain tissue

text: normal cattle brain

text: Plasmid


text: University of North Carolina Wilmington

text: live

text: Generic

text: Total RNA samples were prepared individually from each tissue, checked for quality, and then pooled for construction and normalization of a cDNA library by Invitrogen. Plasmids were isolated and inserts sequenced from their 5'-ends by the Blue Crab Molecular Genetics Laboratory at the University of North Carolina Wilmington. Traces were trimmed, compared (BLASTx) to NCBI non-redundant protein database as of 19 July 2004, and processed for submission to dbEST by trace2dbEST software (Parkinson, Anthony and Blaxter, unpublished software).

text: Adult intermolt

text: Callinectes sapidus

text: Pooled anterior and posterior gills from crabs acclimated to salinities of 35 and 5 parts per thousand

text: Blue crab gill, normalized

text: pCMV Sport 6.1


text: University of Texas at El Paso

text: live

text: Generic

text: Sequenced from suppressive subtractive hybridization products inserted into InVitrogen's pCR 2.1 T/A cloning vector.

text: adult

text: liver

text: Fundulus heteroclitus

text: male

text: Fundulus heteroclitus liver suppressive subtractive hybridization product

text: pCR 2.1 T/A


text: University of Texas at El Paso

text: live

text: Generic

text: Sequenced from a product inserted into InVitrogen's pCR 2.1 T/A cloning vector after deg-PCR.

text: adult

text: liver

text: Fundulus heteroclitus

text: male

text: Fundulus heteroclitus liver deg-PCR

text: pCR 2.1 T/A


text: Sun Yat-sen University, Department of Biology and Biotechnology

text: live

text: Generic

text: Yueyou 523

characteristic: cultivar

text: Mid-matured stage

text: E.coli BM25.8

text: Seed

text: Arachis hypogaea

text: Cotyledons

text: Peanut Lambda Express library


text: Jeonnam Biotechnology Research Center

text: live

text: Generic

text: Nokkwang

text: cDNA library was generated from red ripe fruit pericarp using lambda Zap II phage vector. In vivo excision was done with helper phage to generate subclone in pBluescript II SK(+)XR] vector.

text: Capsicum annuum

text: EcoRI

text: XhoI

text: red pepper fruit pericarp

text: Plasmid

text: pBluescript II SK(+)XR


text: USDA - ARS

text: live

text: Generic

text: Rough Lemon

text: Seedling height 50mm

characteristic: lab-host

text: Citrus jambhiri

text: EcoRI

text: XhoI

text: root

text: Rough Lemon seedling root

text: pBluescript II SK+


text: University of Missouri-Columbia

text: live

text: Generic

text: Funding: A grant from the Monsanto Company to the University of Missouri. Genetic Source: Filamentous whole conceptuses and later stage embryos and fetuses from various stages of development were collected from crossbred pigs (Sus scrofa domestica), frozen in liquid nitrogen immediately after collection, and stored at -80 degrees Celsius until RNA extraction. The specific tissues collected were Day 12 and 14 conceptuses, Day 20 whole embryos and Day 45 fetuses. More information regarding the methods can be found at: http://genome.rnet.missouri.edu/Swine/Methods.html. Library Construction (Standard Protocol): All procedures discussed in this section have been described in detail elsewhere (Soares et al., 1994; Bonaldo et al., 1996; Jiang et al., 2001). Total cellular RNA from each sample was isolated by using STAT-60 reagent (Tel-Test, Friendswood, TX) and poly(A)+ RNA was obtained by two rounds of purification with the Oligotex mRNA isolation kit (Qiagen) according to the manufacturer's instructions. The conceptus libraries and the Day 20 and 45 embryo/fetus libraries were constructed essentially as described by the manufacturer's instructions provided with the SuperScript Plasmid System (Invitrogen, cat. no. 18248-013). Briefly, 1mg of poly(A)+ RNA will be annealed at 37 degrees Celsius with 10mg of NotI-tag-dT18 oligonucleotide (GCTGCTCGCGGCCGC-tag-T18) and reverse transcribed at 37 degrees Celsius with SuperScript II (Invitrogen) reverse transcriptase (Jiang et al., 2001). The 'tag' represents a tissue/stage-specific ten-base sequence identifier (http://genome.uiowa.edu/pubsoft/software.html) present in the oligonucleotide used to prime first-strand synthesis. Second strand synthesis was performed with T4 DNA polymerase in the presence of DNA ligase and RNase H. After second strand synthesis, the double-stranded cDNAs was ligated to SalI adapters (Invitrogen-Life Technologies) and digested with NotI. The cDNAs will be size selected by passage through cDNA size fractionation columns (Invitrogen-Life technologies). The cDNAs derived from each developmental stage of a particular tissue were mixed on an equimolar basis and ligated directionally into the NotI and SalI sites of the pSPORT1 vector (Invitrogen). After ligation of the inserts, the plasmids were electroporated into DH10B bacteria. Preliminary Library Characterization: Randomly chosen clones from each library were analyzed by restriction digestion to determine average insert size (96 clones) and by sequencing (~4 96-well plates) to confirm library quality [e.g. the presence of short polyA+ tails, genomic DNA contamination (must be <1%), ribosomal RNA clones (must be <1%), etc.] and to provide a sequence database representing the predominant clones in each library. The clones were sequenced at the University of Missouri-Columbia DNA Core Facility. Bioinformatics work was performed by GK Springer's bioinformatics group (WG Spollen, JE Ries, A Guillen, AA Khambati, RV Patel, CM Topinka, SB Bhuiyan) in the Computer Science and Health Management and Informatics Departments at the University of Missouri-Columbia. Clone Requests: Requests for clones should be made to the Director of the University of Missouri DNA Core facility at: porcine@rnet.missouri.edu. Citations: 1. Bonaldo MF, Lennon G, Soares MB. Normalization and Subtraction: Two approaches to facilitate gene discovery. Genome Res, 1996; 6:791-806. 2. Jiang H, Bivens NJ, Ries JE, Whitworth KM, Green JA, Forrester LJ, Springer GK, Didion BA, Mathialagan N, Prather RS, Lucy MC (2001) Constructing cDNA libraries with fewer clones that contain long poly(dA) tails. Biotechniques 31:38-42. 3. Soares MB, MF Banaldo, P Jelene, L Su, L Lawton, A Efstrantiadis. 1994. Construction and characterization of a normalized cDNA library. Proc Natl Acad Sci, 91:9228-9232.

text: Day 12 conceptus

text: Sus scrofa

text: Cloning


text: University of Missouri-Columbia

text: live

text: Generic

text: Day 14 conceptus

text: Sus scrofa

text: Cloning


text: University of Missouri-Columbia

text: live

text: Generic

text: Day 20 fetus

text: Sus scrofa

text: Cloning


text: University of Missouri-Columbia

text: live

text: Generic

text: Day 45 fetus

text: Sus scrofa

text: Cloning


text: University of Missouri-Columbia

text: live

text: Generic

text: Funding: A grant from the Monsanto Company to the University of Missouri. Genetic Source: Endometrium and oviduct tissues from various stages of the estrous cycle were collected from crossbred pigs (Sus scrofa domestica), frozen in liquid nitrogen immediately after collection, and stored at -80 degrees Celsius until RNA extraction. The specific tissues collected were Day 0 and Day 3 whole oviducts and Days 3, 6, 10 and 12-14 endometrium. More information regarding the methods can be found at: http://genome.rnet.missouri.edu/Swine/Methods.html. Library Construction (Standard Protocol): All procedures discussed in this section have been described in detail elsewhere (Soares et al., 1994; Bonaldo et al., 1996; Jiang et al., 2001). Total cellular RNA from each sample was isolated by using STAT-60 reagent (Tel-Test, Friendswood, TX) and poly(A)+ RNA was obtained by two rounds of purification with the Oligotex mRNA isolation kit (Qiagen) according to the manufacturer's instructions. The oviduct libraries and the Day 3, 6 and 10 endometrium libraries were constructed essentially as described by the manufacturer's instructions provided with the SuperScript Plasmid System (Invitrogen, cat. no. 18248-013). Briefly, 1mg of poly(A)+ RNA will be annealed at 37 degrees Celsius with 10mg of NotI-tag-dT18 oligonucleotide (GCTGCTCGCGGCCGC-tag-T18) and reverse transcribed at 37 degrees Celsius with SuperScript II (Invitrogen) reverse transcriptase (Jiang et al., 2001). The 'tag' represents a tissue/stage-specific ten-base sequence identifier (http://genome.uiowa.edu/pubsoft/software.html) present in the oligonucleotide used to prime first-strand synthesis. Second strand synthesis was performed with T4 DNA polymerase in the presence of DNA ligase and RNase H. After second strand synthesis, the double-stranded cDNAs was ligated to SalI adapters (Invitrogen-Life Technologies) and digested with NotI. The cDNAs will be size selected by passage through cDNA size fractionation columns (Invitrogen-Life technologies). The cDNAs derived from each developmental stage of a particular tissue were mixed on an equimolar basis and ligated directionally into the NotI and SalI sites of the pSPORT1 vector (Invitrogen). After ligation of the inserts, the plasmids will be electroporated into DH10B bacteria. The day 12-14 endometrium library was synthesized by Dr. Bento Soares' laboratory (University of Iowa) and was cloned into the T3T7pac vector as described elsewhere (Bonaldo et al., 1996). Preliminary Library Characterization: Randomly chosen clones from each library were analyzed by restriction digestion to determine average insert size (96 clones) and by sequencing (~4 96-well plates) to confirm library quality [e.g. the presence of short polyA+ tails, genomic DNA contamination (must be <1%), ribosomal RNA clones (must be <1%), etc.] and to provide a sequence database representing the predominant clones in each library. The clones were sequenced at the University of Missouri-Columbia DNA Core Facility. Bioinformatics work was performed by GK Springer's bioinformatics group (WG Spollen, JE Ries, A Guillen, AA Khambati, RV Patel, CM Topinka, SB Bhuiyan) in Computer Science and Health Management and Informatics Departments at the University of Missouri-Columbia. Clone Requests: Requests for clones should be made to the Director of the University of Missouri DNA Core facility at: porcine@rnet.missouri.edu. Citations: 1. Bonaldo MF, Lennon G, Soares MB. Normalization and Subtraction: Two approaches to facilitate gene discovery. Genome Res, 1996; 6:791-806. 2. Jiang H, Bivens NJ, Ries JE, Whitworth KM, Green JA, Forrester LJ, Springer GK, Didion BA, Mathialagan N, Prather RS, Lucy MC (2001) Constructing cDNA libraries with fewer clones that contain long poly(dA) tails. Biotechniques 31:38-42. 3. Soares MB, MF Banaldo, P Jelene, L Su, L Lawton, A Efstrantiadis. 1994. Construction and characterization of a normalized cDNA library. Proc Natl Acad Sci, 91:9228-9232.

text: Endometrium from a gilt on day 10 of the estrous cycle

text: Sus scrofa

text: Cloning


text: University of Missouri-Columbia

text: live

text: Generic

text: Funding: A grant from the Monsanto Company to the University of Missouri. Genetic Source: Endometrium and oviduct tissues from various stages of the estrous cycle were collected from crossbred pigs (Sus scrofa domestica), frozen in liquid nitrogen immediately after collection, and stored at -80 degrees Celsius until RNA extraction. The specific tissues collected were Day 0 and Day 3 whole oviducts and Days 3, 6, 10 and 12-14 endometrium. More information regarding the methods can be found at: http://genome.rnet.missouri.edu/Swine/Methods.html. Library Construction (Standard Protocol): All procedures discussed in this section have been described in detail elsewhere (Soares et al., 1994; Bonaldo et al., 1996; Jiang et al., 2001). Total cellular RNA from each sample was isolated by using STAT-60 reagent (Tel-Test, Friendswood, TX) and poly(A)+ RNA was obtained by two rounds of purification with the Oligotex mRNA isolation kit (Qiagen) according to the manufacturer's instructions. The oviduct libraries and the Day 3, 6 and 10 endometrium libraries were constructed essentially as described by the manufacturer's instructions provided with the SuperScript Plasmid System (Invitrogen, cat. no. 18248-013). Briefly, 1mg of poly(A)+ RNA will be annealed at 37 degrees Celsius with 10mg of NotI-tag-dT18 oligonucleotide (GCTGCTCGCGGCCGC-tag-T18) and reverse transcribed at 37 degrees Celsius with SuperScript II (Invitrogen) reverse transcriptase (Jiang et al., 2001). The 'tag' represents a tissue/stage-specific ten-base sequence identifier (http://genome.uiowa.edu/pubsoft/software.html) present in the oligonucleotide used to prime first-strand synthesis. Second strand synthesis was performed with T4 DNA polymerase in the presence of DNA ligase and RNase H. After second strand synthesis, the double-stranded cDNAs was ligated to SalI adapters (Invitrogen-Life Technologies) and digested with NotI. The cDNAs will be size selected by passage through cDNA size fractionation columns (Invitrogen-Life technologies). The cDNAs derived from each developmental stage of a particular tissue were mixed on an equimolar basis and ligated directionally into the NotI and SalI sites of the pSPORT1 vector (Invitrogen). After ligation of the inserts, the plasmids will be electroporated into DH10B bacteria. The day 12-14 endometrium library was synthesized by Dr. Bento Soares' laboratory (University of Iowa) and was cloned into the T3T7pac vector as described elsewhere (Bonaldo et al., 1996). Preliminary Library Characterization: Randomly chosen clones from each library were analyzed by restriction digestion to determine average insert size (96 clones) and by sequencing (~4 96-well plates) to confirm library quality [e.g. the presence of short polyA+ tails, genomic DNA contamination (must be <1%), ribosomal RNA clones (must be <1%), etc.] and to provide a sequence database representing the predominant clones in each library. The clones were sequenced at the University of Missouri-Columbia DNA Core Facility. Bioinformatics work was performed by GK Springer's bioinformatics group (WG Spollen, JE Ries, A Guillen, AA Khambati, RV Patel, CM Topinka, SB Bhuiyan) in Computer sCIENCE and Health Management and Informatics Departments at the University of Missouri-Columbia. Clone Requests: Requests for clones should be made to the Director of the University of Missouri DNA Core facility at: porcine@rnet.missouri.edu. Citations: 1. Bonaldo MF, Lennon G, Soares MB. Normalization and Subtraction: Two approaches to facilitate gene discovery. Genome Res, 1996; 6:791-806. 2. Jiang H, Bivens NJ, Ries JE, Whitworth KM, Green JA, Forrester LJ, Springer GK, Didion BA, Mathialagan N, Prather RS, Lucy MC (2001) Constructing cDNA libraries with fewer clones that contain long poly(dA) tails. Biotechniques 31:38-42. 3. Soares MB, MF Banaldo, P Jelene, L Su, L Lawton, A Efstrantiadis. 1994. Construction and characterization of a normalized cDNA library. Proc Natl Acad Sci, 91:9228-9232.

text: Endometrium from pregnant gilts on days 12 & 14 after estrus

text: Sus scrofa

text: Cloning


text: University of Missouri-Columbia

text: live

text: Generic

text: Endometrium from a gilt on day 3 of the estrous cycle

text: Sus scrofa

text: Cloning


text: University of Missouri-Columbia

text: live

text: Generic

text: Oviduct from a gilt on day 3 of the estrous cycle

text: Sus scrofa

text: Cloning


text: University of Missouri-Columbia

text: live

text: Generic

text: Endometrium from a gilt on day 6 of the estrous cycle

text: Sus scrofa

text: Cloning


text: University of Missouri-Columbia

text: live

text: Generic

text: Oviduct from a gilt in estrus

text: Sus scrofa

text: peov

text: Cloning


text: University of Missouri-Columbia

text: live

text: Generic

text: Funding: A grant from the Monsanto Company to the University of Missouri. Genetic Source: Ovarian tissue (whole ovary, dissected follicles, or corpora lutea) was collected from crossbred pigs (Sus scrofa domestica), frozen in liquid nitrogen shortly after collection, and stored at -80 degrees Celsius until RNA extraction. The tissue from several individual pigs was pooled for the purpose of RNA extraction. The specific tissues collected were fetal whole ovary; neonatal whole ovary; prepubutal whole ovary; 2, 4, 6 and 8 mm growing follicles; Day 0 follicles; Day 5 small antral follicles and corpora lutea; Day 12 corpora lutea and Day 12 follicles. More information regarding the methods can be found at: http://genome.rnet.missouri.edu/Swine/Methods.html. Library Construction (Standard Protocol): All procedures discussed in this section have been described in detail elsewhere (Soares et al., 1994; Bonaldo et al., 1996; Jiang et al., 2001). Total cellular RNA from each sample was isolated by using STAT-60 reagent (Tel-Test, Friendswood, TX) and the poly(A)+ RNA was obtained by two rounds of purification with the Oligotex mRNA isolation kit (Qiagen) according to the manufacturer's instructions. The libraries were constructed essentially as described by the manufacturer's instructions provided with the SuperScript Plasmid System (Invitrogen, cat. no. 18248-013). Briefly, 1mg of poly(A)+ RNA will be annealed at 37 degrees Celsius with 10mg of NotI-tag-dT18 oligonucleotide (GCTGCTCGCGGCCGC-tag-T18) and reverse transcribed at 37 degrees Celsius with SuperScript II (Invitrogen) reverse transcriptase (Jiang et al., 2001). The 'tag' represents a tissue/stage-specific ten-base sequence identifier (http://genome.uiowa.edu/pubsoft/software.html) present in the oligonucleotide used to prime first-strand synthesis. Second strand synthesis was performed with T4 DNA polymerase in the presence of DNA ligase and RNase H. After second strand synthesis, the double-stranded cDNAs was ligated to SalI adapters (Invitrogen-Life Technologies) and digested with NotI. The cDNAs will be size selected by passage through cDNA size fractionation columns (Invitrogen-Life technologies). The cDNAs derived from each developmental stage of a particular tissue were mixed on an equimolar basis and ligated directionally into the NotI and SalI sites of the pSPORT1 vector (Invitrogen). After ligation of the inserts, the plasmids will be electroporated into DH10B bacteria. Preliminary Library Characterization: Randomly chosen clones from each library were analyzed by restriction digestion to determine average insert size (96 clones) and by sequencing (~4 96-well plates) to confirm library quality [e.g. the presence of short polyA+ tails, genomic DNA contamination (must be <1%), ribosomal RNA clones (must be <1%), etc.] and to provide a sequence database representing the predominant clones in each library. The clones were sequenced at the University of Missouri-Columbia DNA Core Facility. Bioinformatics work was performed by GK Springer's bioinformatics group (WG Spollen, JE Ries, A Guillen, AA Khambati, RV Patel, CM Topinka, SB Bhuiyan) in Computer Science and Health Management and Informatics Departments at the University of Missouri-Columbia. Clone Requests: Requests for clones should be made to the Director of the University of Missouri DNA Core facility at: porcine@rnet.missouri.edu. Citations: 1. Bonaldo MF, Lennon G, Soares MB. Normalization and Subtraction: Two approaches to facilitate gene discovery. Genome Res, 1996; 6:791-806. 2. Jiang H, Bivens NJ, Ries JE, Whitworth KM, Green JA, Forrester LJ, Springer GK, Didion BA, Mathialagan N, Prather RS, Lucy MC (2001) Constructing cDNA libraries with fewer clones that contain long poly(dA) tails. Biotechniques 31:38-42. 3. Soares MB, MF Banaldo, P Jelene, L Su, L Lawton, A Efstrantiadis. 1994. Construction and characterization of a normalized cDNA library. Proc Natl Acad Sci, 91:9228-9232.

text: 2mm ovarian follicle

text: Sus scrofa

text: Cloning


text: University of Missouri-Columbia

text: live

text: Generic

text: 4mm ovarian follicle

text: Sus scrofa

text: Cloning


text: University of Missouri-Columbia

text: live

text: Generic

text: 6mm ovarian follicle

text: Sus scrofa

text: Cloning


text: University of Missouri-Columbia

text: live

text: Generic

text: 8mm ovarian follicle

text: Sus scrofa

text: Cloning


text: University of Missouri-Columbia

text: live

text: Generic

text: Day 0 ovarian follicle (gilt in standing estrus)

text: Sus scrofa

text: Cloning


text: University of Missouri-Columbia

text: live

text: Generic

text: Day 12 Corpus Luteum

text: Sus scrofa

text: Cloning


text: University of Missouri-Columbia

text: live

text: Generic

text: Day 12 ovarian follicle

text: Sus scrofa

text: Cloning


text: University of Missouri-Columbia

text: live

text: Generic

text: Day 5 ovary (follicle and corpus luteum)

text: Sus scrofa

text: Cloning


text: University of Missouri-Columbia

text: live

text: Generic

text: Fetal ovary

text: Sus scrofa

text: pfeto

text: Cloning


text: University of Missouri-Columbia

text: live

text: Generic

text: Perinatal ovary

text: Sus scrofa

text: pnatal

text: Cloning


text: University of Missouri-Columbia

text: live

text: Generic

text: Peripubertal ovary

text: Sus scrofa

text: pputal

text: Cloning


text: Institute of Biomedical Science, University of Sao Paulo

text: live

text: Generic

text: T lymphocyte

characteristic: cell type

text: Jurkat cells

characteristic: cell-line

text: Isolation of genes regulated by oleic acid treatment of Jurkat cells by selective subtractive hybridization analysis

text: E. coli

text: Homo sapiens

text: Oleic acid SSH Library


text: University of Hannover

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: Holsteiner Cox

text: Total RNAs were isolated from sterile grown Malus domestica cultivar Holsteiner Cox (susceptible for different pathogens e.g. Venturia inaequalis)and from the latter cultivar 24 h after the application of the non-pathogenic bacterium Pseudomonas fluorescens Bk3 to the leaves. The library was constructed using the PCR Select cDNA subtraction kit (Clontech, Palo Alto, CA, USA). Briefly, cDNA was obtained from total RNA using the SMART cDNA synthesis kit (Clontech, Palo Alto, CA, USA) by using an OligodT primer which allowed the amplification of the complete mRNA population contained in each sample. The cDNA populations of the tester (after P.fluorescens Bk3 treatment) and the driver (untreated), were digested with the restriction enzyme RsaI (Gibco, USA) to obtain short blunt-ended fragments. The tester pool was then divided in two populations, and the first one was ligated to the adaptor 1 and the second one to the adaptor 2R, provided with the kit. Each tester pool was hybridised separately with excess driver cDNA, and finally mixed together for a second subtractive hybridisation. The fragments differentially expressed in the tester were then amplified in two PCR reactions, according to the manufacturer's recommendations. The fragments obtained were cloned into the pGEM-T Vector (Promega, Mannheim, Germany), which were used for the transformation of the E. coli XL-1 blue (Stratagene, La Jolla, CA, USA).

text: young leaves

text: E.coli XL-1 blue

text: Malus domestica

text: leaves

characteristic: tissue-type

text: Plasmid

text: pGemT, ampicillin resistant


text: Institute of Chemistry. University of Sao Paulo

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Cloned unidirectionally, 5' end of the cDNA cloned into SalI / NotI site of pSPORT 1. Primer: Oligo (dT). Average insert size: 1 kb; Superscript Plasmid system -5' adaptor sequence: 5'CCCACGCGTCCG3' -3' adaptor sequence: 5'GGCCGCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3'

text: Sporangium 120 minutes after induction of sporulation by starvation

text: E. coli DH5a cells (ampicillin resistant)

text: Blastocladiella emersonii

text: Asexual

text: Plasmid

text: pSPORT 1


text: live

text: Generic

characteristic: NCBI submission package

characteristic: description

text: Sporangium 30 minutes after induction of sporulation by starvation

text: Blastocladiella emersonii

text: Asexual

text: Plasmid

text: pSPORT 1


characteristic: External Id

text: live

text: Generic

text: Sporangium 60 minutes after induction of sporulation by starvation

text: Blastocladiella emersonii

characteristic: organism

text: Asexual

text: Plasmid

text: pSPORT 1


text: live

text: Generic

text: Sporangium 90 minutes after induction of sporulation by starvation

characteristic: dev-stage

text: Blastocladiella emersonii

text: Asexual

characteristic: sex

text: Plasmid

text: pSPORT 1


text: live

text: Generic

text: Germling 120 minutes after induction of germination in DM3 medium

text: Blastocladiella emersonii

text: Asexual

characteristic: title

text: Plasmid

text: pSPORT 1

characteristic: vector


text: live

text: Generic

text: Germling 30 minutes after induction of germination in DM3 medium

characteristic: lab-host

text: Blastocladiella emersonii

text: Asexual

text: Plasmid

text: pSPORT 1


text: live

text: Generic

text: Germling 60 minutes after induction of germination in DM3 medium

text: Blastocladiella emersonii

text: Asexual

text: Plasmid

text: pSPORT 1


text: live

text: Generic

text: Germling 90 minutes after induction of germination in DM3 medium

text: Blastocladiella emersonii

text: Asexual

text: Plasmid

text: pSPORT 1


text: live

text: Generic

text: PCR fragment. Primer: Oligo (dT); PCR-Select cDNA Subtraction system and pGEM T-Easy system. -adaptor sequence 1: 5'CTAATACGACTCACTATAGGGCTCGAGCGGCCGCCCGGGCAGGT3' -adaptor sequence 2R: 5'CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGT3'

text: Vegetative Cell

text: Blastocladiella emersonii

text: Asexual

text: BeNSVP

text: Plasmid

text: pGEM-T Easy


text: live

text: Generic

text: Zoospore

text: Blastocladiella emersonii

text: Asexual

text: BeZSPN

text: Plasmid

text: pSPORT 1


text: University of Illinois

text: live

text: Generic

text: The library Gm-r1089 is a sequence-driven, reracked set of 9,216 low redundancy clones selected from 38 different cDNA libraries constructed from various tissues and stages of development of soybean including 973 cDNAs from germinating cotyledons(source library Gm-c1069, Gm-c1076, and Gm-c1077); 1,465 cDNAs from various tissue and organ systems of the adult plant; 476 cDNAs from adult stem tissue (source library Gm-c1062); 1340 cDNAs from tissue culture derived somatic embryos (source libraries Gm-c1036 and Gm-c1075); 2918 cDNAs from hypocotyls or young seedlings; 742 cDNAs from germinating seedlings, shoot tips, or leaves exposed to various stresses(source libraries Gm-c1065, Gm-c1066, and Gm-c1068); 839 cDNAs from young leaves or hypocotyls exposed to bacterial and fungal pathogens (source libraries Gm-c1072, Gm-c1073, Gm-c1074; and Gm-c1084); and 463 from roots of young plants grown in hydroponic media without phosphate (source library Gm-c1087). The 5' ESTs of the source clones from the different libraries were used to select singletons, or a representative of each contig, which were reracked to form library Gm-r1089 and the cDNA clones of the reracked Gm-r1089 library were then sequenced at the 3' end. The unigene selection and 3' sequencing was funded by NSF Plant Genome project #9872565 (http://soybeangenomics.cropsci.uiuc.edu/) as part of creation of a low redundancy soybean cDNA set. The source cDNA libraries were constructed by the laboratories of Lila Vodkin, University of Illinois, Randy Shoemaker, Iowa State University, and Paul Keim, Northern Arizona University as part of the Public EST project, http://129.186.26.94/soybeanest.html. The contig analysis to select unique genes was performed by the laboratory of Ernest Retzel, Center for Computational Genomics and Bioinformatics, University of Minnesota, http://web.ahc.umn.edu/biodata/nsfsoy/. Reracking and 3' sequencing were conducted by services of the University of Illinois Keck Center for Comparative and Functional Genomics http://www.biotech.uiuc.edu/keck.htm. Note: The corresponding 5' EST from each clone in the Gm-r1089 library is listed in the 'OTHER EST' field. The detailed information on the source library for each clone can also be obtained by referring to the clone ID of the original cDNA library that is also listed under 'OTHER EST'.

text: Glycine max


text: Central Institute of Medicinal and Aromatic Plants

text: live

text: Generic

text: Whole leaf cells

characteristic: cell type

text: Dhawal

characteristic: cultivar

text: PCR based suppression subtractive hybridization was carried out using the cDNA populations derived from the leaf (tester) and root (driver) tissues of Catharanthus roseus at the time of high bisindole alkaloid biosynthesis in the leaf of the plant during the summer season (average day temperature 45oC).

text: Mature six-month-old field grown plant

text: Shoot

characteristic: organ

text: Catharanthus roseus

text: SmaI

text: SmaI

text: Hermaphrodite

text: Leaf

text: Catharanthus roseus subtracted leaf specific cDNA library

text: Phagemid

text: pBluescript II SK(+)


text: Penn State University

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: This is a directionally cloned, non-normalized library. This library has been generated by the Floral Genome Project (FGP). The Floral Genome Project is funded by NSF's Plant Genome Research Program (DBI-0115684). More information about the project can be obtained at http://fgp.bio.psu.edu

text: 5-19 mm buds

text: SOLR

text: Zamia fischeri

text: EcoRI

text: flower buds

text: Phagemid

text: pBluescript SK (+/-)


text: Tea Research Institute, Chinese Academy of Agricultural Sciences

characteristic: INSDC last update

text: live

text: Generic

text: Longjing 43

text: Growing stage

text: E. coli TG1

text: Camellia sinensis

text: SfiIA and B

text: Young leaf

characteristic: tissue-type

text: Young leaf cDNA library of tea plant (Camellia sinensis)

text: Phage

text: lambda TriplEx2


text: Iowa State University

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Catagla

text: cDNA clones from cold acclimated (CA) leaves of Rhododendron catawbiense Michx. RNA for preparation of library was extracted from leaves collected in winter (January 2003) from cold acclimated plants

text: Current year growth on mature field-grown plants

text: XL1-Blue MRF' E. coli strain

text: Leaf

text: Rhododendron catawbiense

text: EcoRI (5' end)

text: XhoI (3' end)

text: Whole leaf

text: cDNA cold acclimated (CA) Rhododendron library

text: Uni-ZAP XR Vector; Uni-ZAP XR vector allows in vivo excision of the pBluescript phagemid


text: Iowa State University

text: live

text: Generic

characteristic: NCBI submission package

text: Catagla

text: cDNA clones from non-acclimated (NA) leaves of Rhododendron catawbiense Michx. RNA for preparation of library was extracted from leaves collected in summer (July 2002) from non-acclimated plants

characteristic: description

text: XL1-Blue MRF' E. coli strain

text: Leaf

text: Rhododendron catawbiense

text: EcoRI (5' end)

text: XhoI (3' end)

text: Whole leaf

text: cDNA non-acclimated (NA) Rhododendron library


characteristic: External Id

text: The Tobacco Science Research Institute of Yunnan Province; Yunnan Provincial Tobacco Group Dali Branch; Bioinfomatics and Gene Network Research Group, Zhejiang University

text: live

text: Generic

text: Nicotiana tabacum

characteristic: organism

text: Mixed

text: Normalized Nicotiana tabacum cDNA library

text: pBS-SK+


text: The University of Georgia, Department of Plant Biology

text: live

text: Generic

text: The library was prepared from polyA+ RNA from the roots of 1-year-old loblolly pine (Pinus taeda) cuttings that were rooted and then planted in washed sand. The rooted cuttings were maintained for 135 days (July 2003 harvest) under ambient conditions in a local greenhouse. They were kept on a weekly regimen of 0.5x nutrient-complete Hoagland's solution and supplemented with additional water sufficient to maintain a 15% soil moisture content. Twenty-four hours (24h) prior to harvesting roots for mRNA preparation, the potted trees were watered with 250 mM NaCl(aq) until the sand was saturated. Double-stranded cDNA was cloned unidirectionally into pSL1180. Inserts can be excised with EcoRI (5' end) and XhoI (3' end).

text: DH10B-T1 phage-resistant E. coli

text: Root

text: Pinus taeda

text: EcoRI

text: XhoI

text: 3 CCLONES

characteristic: strain

text: Roots plus added NaCl


text: Dairy and Swine Research and Development Center, Agriculture and Agri-Food Canada.

text: live

text: Generic

text: Library made from Suppressive Subtractive Hybridization from day-15 Gestating sows.

text: Sus scrofa

text: Female

characteristic: sex

text: Endometrium implantation site

text: Pig endometrial cDNA library

characteristic: title

text: pT-Adv vector


text: Central Institute of Medicinal and Aromatic Plants

text: live

text: Generic

text: Whole placenta

text: Suppression subtractive hybridization was carried out using the cDNA populations derived from the highly pungent fruit placenta (Tester) and non-pungent fruit placenta (Driver) tissues of Capsicum annuum L. The fruit placenta specific messages were cloned on the pBluescript II SK + vector.

text: 30 days after flowering

characteristic: dev-stage

text: E. coli DH5_ strain

characteristic: lab-host

text: Fruit

text: Capsicum annuum

text: EcoR V

text: EcoR V

text: Hermaphrodite

text: Placenta

text: Capsicum annuum L. fruit placenta specific subtracted cDNA library

text: Phagemid

text: pBluescript II SK +

characteristic: vector


text: Commonwealth Scientific and Industrial Research Organisation Division of Plant Industry.

text: live

text: Generic

text: Hartog

characteristic: cultivar

text: mRNA was prepared from endosperm tissues of the wheat cultivar Hartog. cDNA was synthesised from pooled 6, 8, and 10 dpa endosperm using Not I-oligo(dT)18 primer/adapter (Pharmacia Biotech), and then ligated to the Sal I-Not I site of _ZipLox vector (Life Technology) after adding a Sal I-Xho I adapter (Stratagene). Constructed by Shahjahan Ali and Bill Taylor.

text: developing endosperm tissue 6, 8, 10 dpa (days post anthesis)

text: DH10B (Life Technology)

text: Triticum aestivum

text: Sal I

text: Not I

text: endosperm

text: Wheat EST endosperm library

text: Phage


text: Wageningen Centre for Food Research

characteristic: INSDC center name

text: live

text: Generic

text: Aspergillus oryzae

text: Aspergillus oryzae SSF cDNA subtraction library


text: California State University San Marcos

characteristic: INSDC first public

text: live

text: Generic

text: Emiliania huxleyi grown in Artificial Seawater (Guillard's F/2 media).

text: Late log growth phase

text: Emiliania huxleyi

text: Preamplified custom cDNA library in pCMVsport6.1 (ResGen, Invitrogen Inc.)


text: National Institute of Health

characteristic: INSDC last update

text: live

text: Generic

text: HepG2 cells

characteristic: cell type

text: Homo sapiens

text: Liver

text: Differential Display RT-PCR from HepG2 cells


text: National Bureau of Animal Genetics Resources

text: live

characteristic: INSDC status

text: Generic

text: Lactating buffalo mammary gland tissues were processed for RNA isolation and directionally cloned cDNA library was created in pDNR-LIB vector.

text: lactating stage

text: E.coli DH5

text: Bubalus bubalis

text: SfiI

text: SfiI

text: Female

text: Murrah

text: Mammary gland

characteristic: tissue-type

text: cDNA library from lactating buffalo mammary gland

text: Plasmid

text: pDNR -LIB


text: UMR CNRS - UPS 5546

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: country: France:Sud Ouest

text: Eucalyptus gunnii

text: differentiating xylem

text: Eucalyptus gunnii differentiating xylem