text: University of Calgary

text: live

text: Generic

text: embryonic stem (ES) cell

characteristic: cell type

characteristic: cell-line

text: Library constructed by Dr. Gerard Bain (present address: Hoechst-ARIAD Genomic Center, ARIAD Pharmaceuticals Inc., 26 Landsdowne Street, Cambridge, Massachusetts, 02139-4234, U.S.A.). To isolate cDNAs corresponding to mRNAs which are upregulated during the neural differentiation of ES cells in vitro, the subtractive hybridization technique of Wang and Brown [1] was employed. Poly(A)+ RNA was prepared from both undifferentiated ES cells and from embryoid bodies which had been cultured for 4 days in the absence of RA followed by an additional 3 days in the presence of 0.5 (M RA (4-/3+ cells). These poly(A)+ RNAs were converted to double-stranded cDNA using the Superscript Choice System (Gibco). Aliquots of both cDNAs were digested with the restriction enzymes AluI and AluI plus RsaI. An adaptor oligo [1] containing an EcoRI site was ligated to the ends of the restricted cDNAs to provide primer binding sites and large amounts of each cDNA population were then produced by the polymerase chain reaction (PCR) as described [1]. Amplified cDNA from undifferentiated ES cells was biotinylated using Photoprobe biotin (Vector Laboratories) according to the manufacturer's protocol. 2.5 ug of amplified cDNA from 4-/3+ cells was mixed with 50 ug of biotinylated ES cell cDNA, denatured by boiling, and hybridized for 20 h. Double stranded cDNAs containing biotin were removed by streptavidin/phenol treatment as described [1]. The remaining subtracted cDNA was mixed with an additional 25 mg of biotinylated ES cell cDNA, denatured by boiling, and hybridized for 2 h. The streptavidin/phenol treatment was repeated and the remaining cDNA was amplified by PCR [Wang and Brown, 1991]. Two additional rounds of subtraction were repeated exactly as described above. The cDNA obtained from this subtraction procedure was digested with EcoRI and ligated to pBS II SK+ (Stratagene) followed by transformation into E. coli DH5 cells. Individual colonies were picked and the corresponding plasmids were isolated either by an alkaline lysis miniprep procedure [2], or using the Qiaprep spin miniprep kit (Qiagen). Sequence analysis was performed using the Big Dye Cycle Sequencing kit and an ABI373 sequencer (University Core DNA Services, University of Calgary). 1. Wang,Z; Brown,DD (1991) A gene expression screen. Proc. Natl. Acad. Sc i. USA 88, 11505-11509. 2. Sambrook,J; Fritsch,EF; Maniatis,T. (1989) Molecular Cloning:A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor,New York.

text: DH5 alpha

text: Mus musculus

text: Bain Rancourt retinoic acid induced ES cell neural differentiation subtraction library

text: plasmid

text: pBluescript II SK+ (Stratagene)


status: PUBLIC

characteristic: External Id

text: University of Illinois at Urbana-Champaign

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Adult

tag: attribute

characteristic: dev-stage

text: Ovary

characteristic: organ

text: Bos taurus

characteristic: organism

text: Female

characteristic: sex

text: Angus

characteristic: strain

text: Lewin Cattle Ovary

characteristic: title

text: phagemid

text: pBluescript SK(+/-)

characteristic: vector


text: Children's Memorial Research Center

text: live

text: Generic

text: The UI-R-BJ0 library is a subtracted library derived from the UI-R-AA1, UI-R-AB1, UI-R-AC1, UI-R-AD1, UI-R-AE1, UI-R-AF1, and UI-R-AG1 libraries. These libraries represent tissues from rat atrium at 16.5 dpc, ventricle at 16.5 dpc, AV canal at 16.5 dpc, atrium at 15 dpc, ventricle at 15 dpc, AV canal at 15 dpc, and ventricle at 13 dpc. The tag is a string of 5-6 nucleotides present between the Not I site and the oligo-dT track. The library was constructed as described by Bonaldo, Lennon and Soares, Genome Research 6: 791-806, 1996.

characteristic: description

text: adult

text: DH10B (Life Technologies)

characteristic: lab-host

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid


text: Children's Memorial Research Center

text: live

text: Generic

text: The UI-R-BJ0p library is a subtracted library derived from the UI-R-AA1, UI-R-AB1, UI-R-AC1, UI-R-AD1, UI-R-AE1, UI-R-AF1, and UI-R-AG1 libraries. These libraries represent tissues from rat atrium at 16.5 dpc, ventricle at 16.5 dpc, AV canal at 16.5 dpc, atrium at 15 dpc, ventricle at 15 dpc, AV canal at 15 dpc, and ventricle at 13 dpc. The tag is a string of 5-6 nucleotides present between the Not I site and the oligo-dT track. The library was constructed as described by Bonaldo, Lennon and Soares, Genome Research 6: 791-806, 1996.

text: adult

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid


text: Institute of Biological Chemistry/Washington State University

text: live

text: Generic

text: secretory

characteristic: cell type

text: Black Mitcham

characteristic: cultivar

text: Mentha x piperita

text: peltate glandular trichomes

characteristic: tissue-type

text: peppermint glandular trichome

text: phagemid

text: lamba ZAPII


characteristic: External Id

text: Institute of Genetic Diagnosis, the Fourth Military Medical University

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: gastric epithelial cell GES-1

text: differentially expressed gene sequences from human gastric epithelial cell GES-1. The method used is DDRT-PCR. The control cell line is human gastric carcinoma cell GC7901

text: Homo sapiens

text: gastric epithelial cell GES-1


text: University of Massachusetts Medical School

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Clontech Marathon Ready RACE cDNA Library, Cat number 7475-1

text: 10-12 weeks

text: Rattus norvegicus

text: male

text: Sprague-Dawley

text: testis

text: Clontech number 7475-1


text: Centre National de la Recherche Scientifique (CNRS)

text: live

text: Generic

characteristic: NCBI submission package

text: developing young nodule

characteristic: dev-stage

text: Medicago truncatula

text: symbiotic root nodule

text: Medicago truncatula R108

text: NOLLY Lambda HybriZAP


text: live

text: Generic

text: developing young nodule

text: Medicago truncatula

characteristic: organism

text: symbiotic root nodule

text: Medicago truncatula R108MtNo062

text: NOLLY Lambda HybriZAP


text: live

text: Generic

text: developing young nodule

text: Medicago truncatula

characteristic: strain

text: symbiotic root nodule

text: Medicago truncatula R108MtNo

text: NOLLY Lambda HybriZAP


text: live

text: Generic

text: developing young nodule

text: Medicago truncatula

text: symbiotic root nodule

text: Medicago truncatula R108MtNo079

characteristic: title

text: NOLLY Lambda HybriZAP


text: live

text: Generic

text: developing young nodule

text: Medicago truncatula

text: symbiotic root nodule

text: Medicago truncatula R108MtNo084

text: NOLLY Lambda HybriZAP

characteristic: vector


text: live

text: Generic

text: developing young nodule

text: Medicago truncatula

text: symbiotic root nodule

text: Medicago truncatula R108MtNo122

text: NOLLY Lambda HybriZAP


text: live

text: Generic

text: developing young nodule

text: Medicago truncatula

text: symbiotic root nodule

text: Medicago truncatula R108Mt

text: NOLLY Lambda HybriZAP


text: live

text: Generic

text: developing young nodule

text: Medicago truncatula

text: symbiotic root nodule

text: Medicago truncatula R108MtNo195

text: NOLLY Lambda HybriZAP


text: live

text: Generic

text: developing young nodule

text: Medicago truncatula

text: symbiotic root nodule

text: Medicago truncatula R108MtNo316

text: NOLLY Lambda HybriZAP


text: live

text: Generic

text: developing young nodule

text: Medicago truncatula

text: symbiotic root nodule

text: Medicago truncatula R108MtNo323

text: NOLLY Lambda HybriZAP


text: live

text: Generic

text: developing young nodule

text: Medicago truncatula

text: symbiotic root nodule

text: Medicago truncatula R108MtNo361

text: NOLLY Lambda HybriZAP


text: Jikei University School of Medicine

text: live

text: Generic

text: rIL-2 activated normal lymphocytes

text: T/A cloning of the differentially displayed cDNAs

characteristic: description

text: Homo sapiens

text: male

characteristic: sex

text: cDNA Library from rIL-2 activated lymphocytes

text: Plasmid


text: Washington University School of Medicine

text: live

text: Generic

text: cDNA made by oligo-dT priming. Directionally cloned into EcoRI/NotI sites using the following 5' adaptor: gaattccccggg and 3' adaptor: agagaactagtgtcgacgcggccgcttttttttttttttttttt. Vector designed for mRNA injections (reference Lemaire et al. (1995) 81:85-94). Average insert size 1.6kb. Primary library, non-amplified. Library constructed by N. Pollet, PhD REF: Glinka, et al. (1996) Combinatorial signaling by Xwnt-11 and Xnr3 in the organizer epithelium. Mech. Dev. 60:221-231. Note: This is a Xenopus Gene Collection (XGC) library.

text: stage 13

text: XL1-Blue, F+

characteristic: lab-host

text: Xenopus laevis

text: NotI

text: EcoRI

text: whole embryo, hyperdorsalized by LiCl treatment

text: Xenla 13LiCl

text: plasmid (ampicillin resistant)

text: pBSRN3 (modified pBluescript KS+, see description below)


text: University of Kentucky

text: live

text: Generic

text: epidermis/dermis

characteristic: cell type

text: Poly dT column purified mRNA was used to make a directionally inserted expression cDNA library. The library had 5.8X 105 pfu with 99% recombinant phage before amplification. The average insert size is 1.3 kbp.

text: Equus caballus

text: skin

text: Horse Lambda Zap Express Library


text: live

text: Generic

text: cDNA made by oligo-dT priming. Non-directionally cloned into the UDG sites of pAMP10. Size-selected on agarose gel, average insert size 500 bp. Primary library; non-amplified. cDNA Library Preparation: David B. Krizman, Ph.D (NCI). Reference: Krizman et al. (1996) Cancer Research 56:5380-5383.

text: colon

characteristic: organ

text: Homo sapiens

text: colonic adenocarcinoma

characteristic: tissue-type

text: plasmid (ampicillin resistant)


characteristic: External Id

text: live

text: Generic

text: ovary

text: Homo sapiens

text: female

text: endometrioid ovarian metastasis

text: plasmid (ampicillin resistant)


text: Lawrence Livermore National Laboratory

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: Homo sapiens

text: normal squamous epithelium, floor of mouth

text: plasmid (ampicillin resistant)


text: Lawrence Livermore National Laboratory

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: tongue

text: Homo sapiens

text: normal squamous epithelium

text: plasmid (ampicillin resistant)


text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: tongue

text: Homo sapiens

text: moderate to poorly differentiated invasive carcinoma

text: plasmid (ampicillin resistant)


text: Seoul National University

text: live

text: Generic

text: Nicotiana tabacum

characteristic: organism

text: Nicotiana tabacum library (Choi G)


text: C.R.H.D.Q., Univ. Laval

text: live

text: Generic

text: LNCaP prostate cancer

characteristic: cell-line

text: Homo sapiens

text: Homo sapiens LNCaP prostate cancer


text: Sun Yat-sen University of Medical Sciences

text: live

text: Generic

text: Epithelium adenocarcinoma

text: LoVo

text: The human colon cancer LoVo cells were induced by 10-5 mol/L all-trans-retinoic acid and 10-7 mol/L 1,25-dihydroxy vitamin D3 for 2 days. Treated cell cDNA was used as driver and untreated cell cDNA as tester. Suppression subtractive hybridization (Clontech PCR-select cDNA subtraction kit) was used to screening the differentially exprssed genes. The differentially expressed sequences amplified were directly cloned into pT-Adv (Clontech advanTAge PCR cloning kit) and transformated into E. Coli DH5a cells. The positive clones were sequenced by using M13 reverse primer or T7 promoter primer.

text: E. coli DH5a

text: Colon

text: Homo sapiens

text: Eco RI

text: Eco RI

text: LoVo express library before ATRA and D3 induced

characteristic: title

text: Plasmid

text: pT-Adv


text: live

text: Generic

text: Epithelium adenocarcinoma

text: LoVo

text: The human colon cancer LoVo cells were induced by 10.0u mol/L all-trans-retinoic acid and 0.1u mol/L 1,25-dihydroxy vitamin D3 for 2 days. Treated cell cDNA was used as tester and untreated cell cDNA as driver. Suppression subtractive hybridization (Clontech PCR-select cDNA subtraction kit) was used to screening the differentially exprssed genes. The differentially expressed sequences amplified were directly cloned into pT-Adv (Clontech advanTAge PCR cloning kit) and transformated into E. coli DH5a cells. The positive clones were sequenced by using M13 reverse primer or T7 promoter primer

text: E. coli DH5a

text: Colon

text: Homo sapiens

text: Eco RI

text: Eco RI

text: LoVo express library after ATRA and D3 induced

text: Plasmid

text: pT-Adv

characteristic: vector


text: Bio College Kyoto

text: live

text: Generic

text: seedling

characteristic: dev-stage

text: Vigna angularis

text: epicotyl

text: Vigna angularis Lambda gt 10 cDNA library, EVA01


text: Genoscope - CNS

text: live

text: Generic

text: embryo

text: Mus musculus

text: primitive streak

text: Mus musculus primitive streak embryo


text: Genoscope - CNS

text: live

text: Generic

text: embryo

text: Mus musculus

text: embryonic region

text: Mus musculus embryonic region


text: Genoscope - CNS

text: live

text: Generic

text: embryo

text: Mus musculus

text: endoderm

text: Mus musculus embryo endoderm


text: Institute of Genetic Diagnosis, the Fourth Military Medical University

text: live

text: Generic

text: gastric carcinoma cell GC7901

text: differentially expressed gene sequences from human gastric carcinoma cell GC7901 . The method used is DDRT-PCR. The control cell line is gastric epithelial cell GES-1

characteristic: description

text: Homo sapiens

text: gastric carcinoma cell GC7901


text: University of Nevada

text: live

text: Generic

text: Six week old

text: Mesembryanthemum crystallinum

text: EcoRI

text: XhoI

text: Leaf, 48 h 0.4M NaCl

text: Ice plant Lambda Uni-Zap XR expression library, 48 hours NaCl treatment

text: Phagemid

text: Lambda Uni-Zap XR, Bluescript SK-


text: Stanford University

text: live

text: Generic

characteristic: cultivar

text: cDNA library of silks infected with 1 microliter of 500,000 spores/ml solution of Fusarium graminearum DAOM 180378. Prepared by Sharon Allard of Eastern Cereal and Oilseed Research Centre, Agriculture and Agri-Food Canada using Stratagene cDNA synthesis kit. Silk was harvested at 72 hours p.i.

text: adult

characteristic: lab-host

text: silk

text: Zea mays

text: XhoI

text: EcoRI

text: silk

text: 829 - Silk infected with Fusarium

text: plasmid

text: pBluescript II XR


text: Universidad de Colima

text: live

text: Generic

text: Phaseolus vulgaris

text: root

text: Phaseolus vulgaris root


text: University of Cincinnati

text: live

text: Generic

text: Mesocricetus auratus

text: Mesocricetus auratus library (Lehman MN)


text: live

text: Generic

text: lung

characteristic: organ

text: Homo sapiens

text: large cell carcinoma

text: plasmid (ampicillin resistant)


text: live

text: Generic

text: ovary

text: Homo sapiens

text: female

characteristic: sex

text: papillary serous ovarian metastasis

characteristic: tissue-type

text: plasmid (ampicillin resistant)


characteristic: External Id

text: Johns Hopkins University

characteristic: INSDC center name

text: live

text: Generic

text: SalI-NotI fragment isolated from gal4 fusion protein expression library; isolated from organism receiving single electroconvulsive shock

text: Rattus norvegicus

text: brain

text: Rattus norvegicus brain SalI-NotI fragment


text: The University of Georgia, Department of Plant Biology

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: The library was made from poly-A RNA in the cloning vector lambda ZAP II. Clones to be sequenced were prepared by mass excision.

text: 10- to 14-day-old light-grown (greenhouse) seedlings

text: Sorghum bicolor

text: XhoI

text: EcoRI

text: Light Grown 1 (LG1)

text: Plasmid

text: Lambda Zap


text: Simon Fraser University

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: mRNA isolated from drill-wounded bark tissues; RT-PCR product

text: Picea sitchensis

text: bark

text: Sitka spruce drill-wounded bark


text: International Centre for Genetic Engineering and Biotechnology

text: live

text: Generic

characteristic: NCBI submission package

text: Pusa bold

text: Brassica juncea

characteristic: organism

text: Mannitol Stress inducible cDNA lib of Brassica juncea cv Pusa bold

text: lambdaZapII


text: Jawaharlal Nehru University

text: live

text: Generic

text: xanthi

text: Nicotiana tabacum

text: Nicotiana tabacum cv. xanthi

characteristic: title


text: Cardinal Bernardin Cancer Center

text: live

text: Generic

text: endothelial cell

characteristic: cell type

text: Homo sapiens

text: Homo sapiens endothelial cell


text: Arizona Cancer Center, University of AZ

text: live

text: Generic

characteristic: cell-line

text: Homo sapiens

text: human melanoma microcell hybrid line, MelJuSo-28(1n)


text: Arizona Cancer Center, University of AZ

characteristic: INSDC secondary accession

text: live

text: Generic

characteristic: SRA accession

text: melanoma line, MelJuSo

text: Homo sapiens

text: human melanoma line, MelJuSo


text: University of Edinburgh

text: live

text: Generic

text: Trichuris muris is a nematode parasite of rodents related to the human whipworm Trichuris trichiura. The library was constructed from Trichuris muris adults (Edinburgh 'E' strain) maintained in mice, and was provided by Dr. Richard Grencis, University of Manchester.

characteristic: description

text: adult

characteristic: dev-stage

text: Trichuris muris

text: EcoRI (5'end)

text: XhoI (3'end)

text: mixed

text: Trichuris muris (parasitic nematode) mixed adult

text: Phage

text: Lambda Zap II

characteristic: vector


text: Shanghai Tiedao University Medical School

text: live

text: Generic

text: neuron and neurofiber

text: adult rat

text: Rattus norvegicus

text: male

characteristic: sex

text: Sprague-Dawley

characteristic: strain

text: cerebellum

characteristic: tissue-type

text: Subtraction library of cerebellum


characteristic: External Id

text: Institution of Medical Biology, CAMS

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: Fibroblast cDNA library induced by binding of HSV

text: lung

characteristic: organ

text: Homo sapiens

text: FIBHSVI


text: Institution of Medical Biology, CAMS

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Fibroblast cDNA library induced by WCTGF

text: lung

text: Homo sapiens

text: FIBCTGF


text: Institution of Medical Biology, CAMS

text: live

text: Generic

text: Fibroblast cDNA library induced by binding HAV

text: liver

text: Homo sapiens

characteristic: organism

text: FIBHAV

characteristic: title


text: Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais

text: live

text: Generic

text: sequences obtained by differential display approach

text: adult

text: Schistosoma mansoni

text: female

text: LE

text: Schistosoma mansoni LE differential display


text: University of Illinois at Urbana-Champaign

text: live

text: Generic

text: Adult

text: Spleen

text: Bos taurus

text: Female

text: Angus

text: Lewin Cattle Spleen

text: phagemid

text: pBluescript SK(+/-)


text: North Carolina State University

text: live

text: Generic

text: The sequences contain a 'cDNA adapter' between the EcoRI site and the start of the EST. The adapter sequence is 'AATTCGGCACGAG'.

text: Pinus taeda

text: Eco RI

text: Nsf Xylem Normal wood Vertical

text: BlueScript SK


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: NCI_CGAP_Sub6 is a subtracted library derived from BW, which consists of a mixture of four normalized libraries: NCI_CGAP_Brn50, NCI_CGAP_Lu13, NCI_CGAP_Ov18, GBC1 . The NCI_CGAP_Sub6 library had 7 million recombinants. A single-stranded DNA preparation of BW was used as a tracer in a subtractive hybridization with a driver comprising: the IMAGE pool (NCI_CGAP_Kid3 pool 1 LLAM 3334-3337, 3682-3683, 3798-3803 (IMAGE CloneIDs 1322376-1323911, 1456008-1456775,1500552-1502855); NCI_CGAP_Kid5 pool 1 LLAM 3338-3342,3722-3725, 3776-3778 (IMAGE CloneIDs 1323912-1325831, 1471368-1472903, 1492104-1493255); NCI_CGAP_Lu5 pool 1 LLAM 3575-3582, 3851-3854 (IMAGE CloneIDs 1414920-1417991, 1520904-1522439); NCI_CGAP_GC4 pool 1 LLAM 3164-3167, 3716-3720, 3733-3735 (IMAGE CloneIDs 1257096-1258631,1469064-1470983, 1475592-1476743); NCI_CGAP_Pr22 pool 1 LLAM 2457-2459, 2758-2759, 3062-3068 (IMAGE CloneIDs 985608-986759, 1101192-1101959, 1217928-1220615); NCI_CGAP_Co10 pool 1 LLAM 2644-2653, 2871-2872 (IMAGE CloneIDs 1057416-1061255,1144584-1145351). (50% of the driver population), plus a pool of 3,840 arrayed clones from NCI_CGAP_Sub1 (IMAGE CloneIDs 2708616-2710535) and NCI_CGAP_Sub2 (IMAGE CloneIDs 2710536-2712455) (20% of the driver population), plus a pool of 11,136 clones from NCI_CGAP_Sub3 (IMAGE CloneIDs 2712456-2723591) (30% of the driver population). Subtraction was performed as previously described [Bonaldo, Lennon & Soares (1996): Normalization and Subtraction: Two Approaches To Facilitate Gene Discovery. Genome Research 6, 791-806.

text: DH10B (Life Technologies)

characteristic: lab-host

text: Homo sapiens

text: Not I

text: Eco RI

text: Phagemid


text: live

text: Generic

text: NCI_CGAP_Sub5 is a subtracted library derived from NCI_CGAP_Sub4. The NCI_CGAP_Sub5 library had 3 million recombinants. A single-stranded DNA preparation of NCI_CGAP_Sub4 was used as a tracer in a subtractive hybridization with a driver comprising: the IMAGE pool (NCI_CGAP_Kid3 pool 1 LLAM 3334-3337, 3682-3683, 3798-3803 (IMAGE CloneIDs 1322376-1323911, 1456008-1456775,1500552-1502855); NCI_CGAP_Kid5 pool 1 LLAM 3338-3342,3722-3725, 3776-3778 (IMAGE CloneIDs 1323912-1325831, 1471368-1472903, 1492104-1493255); NCI_CGAP_Lu5 pool 1 LLAM 3575-3582, 3851-3854 (IMAGE CloneIDs 1414920-1417991,1520904-1522439); NCI_CGAP_GC4 pool 1 LLAM 3164-3167, 3716-3720, 3733-3735 (IMAGE CloneIDs 1257096-1258631,1469064-1470983, 1475592-1476743); NCI_CGAP_Pr22 pool 1 LLAM 2457-2459, 2758-2759, 3062-3068 (IMAGE CloneIDs 985608-986759,1101192-1101959, 1217928-1220615); NCI_CGAP_Co10 pool 1 LLAM 2644-2653, 2871-2872 (IMAGE CloneIDs 1057416-1061255,1144584-1145351). (10% of the driver population), plus a pool of 3,840 arrayed clones from NCI_CGAP_Sub1 (IMAGE CloneIDs 2708616-2710535) and NCI_CGAP_Sub2 (IMAGE CloneIDs 2710536-2712455) (10% of the driver population), plus a pool of 11,136 clones from NCI_CGAP_Sub3 (IMAGE CloneIDs 2712456-2723591) (10% of the driver population), plus a pool of 5,472 clones from NCI_CGAP_Sub4 (IMAGE CloneIDs 2723592-2728969) (70% of the driver population). Subtraction was performed as previously described [Bonaldo, Lennon & Soares (1996): Normalization and Subtraction: Two Approaches To Facilitate Gene Discovery. Genome Research 6, 791-806.

characteristic: description

text: DH10B (Life Technologies)

text: Homo sapiens

text: Not I

text: Eco RI

text: Phagemid


text: live

text: Generic

text: NCI_CGAP_Sub7 is a subtracted library derived from NCI_CGAP_Sub6. The NCI_CGAP_Sub7 library had 12 million recombinants. A single-stranded DNA preparation of NCI_CGAP_Sub6 was used as a tracer in a subtractive hybridization with a driver comprising: the IMAGE pool (NCI_CGAP_Kid3 pool 1 LLAM 3334-3337, 3682-3683, 3798-3803 (IMAGE CloneIDs 1322376-1323911, 1456008-1456775,1500552-1502855); NCI_CGAP_Kid5 pool 1 LLAM 3338-3342,3722-3725, 3776-3778 (IMAGE CloneIDs 1323912-1325831, 1471368-1472903, 1492104-1493255); NCI_CGAP_Lu5 pool 1 LLAM 3575-3582, 3851-3854 (IMAGE CloneIDs 1414920-1417991, 1520904-1522439); NCI_CGAP_GC4 pool 1 LLAM 3164-3167, 3716-3720, 3733-3735 (IMAGE CloneIDs 1257096-1258631,1469064-1470983, 1475592-1476743); NCI_CGAP_Pr22 pool 1 LLAM 2457-2459, 2758-2759, 3062-3068 (IMAGE CloneIDs 985608-986759, 1101192-1101959, 1217928-1220615); NCI_CGAP_Co10 pool 1 LLAM 2644-2653, 2871-2872 (IMAGE CloneIDs 1057416-1061255,1144584-1145351). (6% of the driver population), plus a pool of 3,840 arrayed clones from NCI_CGAP_Sub1 (IMAGE CloneIDs 2708616-2710535) and NCI_CGAP_Sub2 (IMAGE CloneIDs 2710536-2712455) (4% of the driver population), plus a pool of 11,136 clones from NCI_CGAP_Sub3 (IMAGE CloneIDs 2712456-2723591) (10% of the driver population), plus a pool of 5,472 clones from NCI_CGAP_Sub4 (IMAGE CloneIDs 2723592-2729326) (40% of the driver population), plus a pool of 4032 clones from NCI_CGAP_Sub6 (IMAGE CloneIDs 2728969-2733190) (40% of the driver population). Subtraction was performed as previously described [Bonaldo, Lennon & Soares (1996): Normalization and Subtraction: Two Approaches To Facilitate Gene Discovery. Genome Research 6, 791-806.

text: DH10B (Life Technologies)

text: Homo sapiens

text: Not I

text: Eco RI

text: Phagemid


text: USDA, ARS, US Meat Animal Research Center

text: live

text: Generic

text: Library made from pooled tissue from lymph node, ovary, fat, hypothalamus, and pituitary.

text: Bos taurus

text: NotI

text: SalI

text: pooled

text: MARC 1BOV

text: plasmid

text: pCMV SPORT6


text: live

text: Generic

text: Library made from pooled tissue from testis, thymus, semitendonosus muscle, longissimus muscle, pancreas, adrenal, and endometrium.

text: Bos taurus

text: NotI

text: SalI

text: pooled

text: MARC 2BOV

text: plasmid

text: pCMV SPORT6


text: live

text: Generic

text: Library made from pooled tissue from marrow, alveolar macrophage, ovary, fetal semitendonosus muscle, and fetal longissimus muscle.

text: Bos taurus

text: NotI

text: SalI

text: pooled

text: MARC 3BOV

text: plasmid

text: pCMV SPORT6


text: live

text: Generic

text: Library made from pooled tissue from day 20 and day 40 embryos.

text: Bos taurus

text: NotI

text: SalI

text: pooled

text: MARC 4BOV

text: plasmid

text: pCMV SPORT6


text: live

text: Generic

text: Library made from pooled tissue from day 11, 13, 15, 20, and 30 embryos.

text: Sus scrofa

text: NotI

text: SalI

text: pooled

text: MARC 1PIG

text: plasmid

text: pCMV SPORT6


text: live

text: Generic

text: Library made from pooled tissue from testis, ovary, endometrium, hypothalamus, pituitary, and placenta.

text: Sus scrofa

text: NotI

text: SalI

text: pooled

text: MARC 2PIG

text: plasmid

text: pCMV SPORT6


text: Otto-von-Guericke University Magdeburg

text: live

text: Generic

text: Homo sapiens

text: inflammatory skin

text: Homo sapiens inflammatory skin


text: Otto-von-Guericke University Magdeburg

text: live

text: Generic

text: Homo sapiens

text: psoriatic skin

text: Homo sapiens psoriatic skin


text: Seoul National Univeristy

text: live

text: Generic

text: adult

characteristic: dev-stage

text: Rattus norvegicus

text: Sprague-Dawley

text: hypothalamus

text: Rattus norvegicus hypothalamus Sprague-Dawley adult


text: Genoscope - Centre National de Sequencage

text: live

text: Generic

text: 1st strand cDNA was primed with a NotI-oligo(dT) primer. Five prime end enriched, double-stranded cDNA was digested with Not I and cloned into the Not I and Eco RV sites of the pCMVSPORT 6 vector. Library is not normalized, but is the control for the normalized libraries. Library was constructed by Life Technologies. Contact : Feng Liang Life Technologies, a division of Invitrogen 9800 Medical Center Drive Rockville, Maryland 20850, USA Fax : (1) 301 610 8371 Email : fliang@lifetech.com URL : http://fulllength.invitrogen.com

text: brain

text: Homo sapiens

text: male

text: neuroblastoma cells

text: phagemid (ampicillin resistant)

text: pCMVSPORT 6

characteristic: vector


text: live

text: Generic

text: 1st strand cDNA was primed with a NotI-oligo(dT) primer. Five prime end enriched, double-stranded cDNA was digested with Not I and cloned into the Not I and Eco RV sites of the pCMVSPORT 6 vector. Library was normalized. Library was constructed by Life Technologies. Contact : Feng Liang Life Technologies, a division of Invitrogen 9800 Medical Center Drive Rockville, Maryland 20850, USA Fax : (1) 301 610 8371 Email : fliang@lifetech.com URL : http://fulllength.invitrogen.com

text: brain

text: Homo sapiens

text: male

characteristic: sex

text: neuroblastoma cells

text: phagemid (ampicillin resistant)

text: pCMVSPORT 6


text: live

text: Generic

text: 1st strand cDNA was primed with a NotI-oligo(dT) primer. Five prime end enriched, double-stranded cDNA was digested with Not I and cloned into the Not I and Eco RV sites of the pCMVSPORT 6 vector. Library was constructed by Life Technologies. Contact : Feng Liang Life Technologies, a division of Invitrogen 9800 Medical Center Drive Rockville, Maryland 20850, USA Fax : (1) 301 610 8371 Email : fliang@lifetech.com URL : http://fulllength.invitrogen.com

text: Homo sapiens

text: male

text: B cells from Burkitt lymphoma

text: phagemid (ampicillin resistant)

text: pCMVSPORT 6


text: live

text: Generic

text: Homo sapiens

text: T cells from T cell leukemia

text: phagemid (ampicillin resistant)

text: pCMVSPORT 6


text: live

text: Generic

text: placenta

text: Homo sapiens

text: phagemid (ampicillin resistant)

text: pCMVSPORT 6


text: live

text: Generic

text: pooled tissue from post conception fetuses (20 week, 24 week and 26 week)

text: Fetal brain

text: Homo sapiens

text: phagemid (ampicillin resistant)

text: pCMVSPORT 6


text: Washington University School of Medicine

text: live

text: Generic

text: Williams

characteristic: cultivar

text: This cDNA library was constructed from mRNA isolated from etiolated hypocotyl tissue of 9-10 day old seedlings. Complementary DNA was synthesized from mRNA using a poly (dT) primer with a XhoI restriction site. EcoRI adapters were ligated to the blunt-ended cDNA fragments followed by digestion with EcoRI and XhoI. The cDNA fragments were directionally cloned into the EcoRI-XhoI restriction site of the pT7T3-Pac vector. The ligated cDNA fragments were transformed into DH10B host cells (Gibco BRL). This library was constructed by Dr. Randy Shoemaker.

text: Glycine max

text: EcoRI

text: XhoI

text: Hypocotyl, 9-10 day old etiolated seedlings

text: plasmid

text: pT7T3Pac (pT7T3, Pharmacia)


text: live

text: Generic

text: Williams 82

text: This cDNA library was constructed from mRNA isolated from hypocotyl tissue of 3 day old seedlings. Complementary DNA was synthesized from mRNA using a poly (dT) primer with a NotI restriction site. EcoRI adapters were ligated to the blunt-ended cDNA fragments followed by digestion with EcoRI and NotI. The cDNA fragments were directionally cloned into the EcoRI-NotI restriction site of the pT7T3-Pac vector. The ligated cDNA fragments were transformed into DH10B host cells (Gibco BRL). This library was constructed by Dr. Randy Shoemaker.

text: Glycine max

text: EcoRI

text: NotI

text: Hypocotyl, 3 day seedlings

characteristic: tissue-type

text: plasmid

text: pT7T3Pac (pT7T3, Pharmacia)


characteristic: External Id

text: ITBA CNR

text: live

text: Generic

text: LA7 derived from RAMA 25 (mammary)

characteristic: cell-line

text: Rattus norvegicus

text: Sprague-Dawley

characteristic: strain

text: Rattus norvegicus LA7 derived from RAMA 25 (mammary)


text: ITBA CNR

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: 106AA10 derived from RAMA 25 (mammary)

text: Rattus norvegicus

text: Sprague-Dawley

text: Rattus norvegicus 106AA10 derived from RAMA 25 (mammary)


text: ITBA CNR

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Rattus norvegicus

text: Sprague-Dawley

text: mammary

text: Rattus norvegicus mammary Sprague-Dawley


text: Virginia Tech

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: embryonic ten-day-old

text: Gallus gallus

text: female

text: White Leghorn

text: whole embryo

text: Normalized cDNA library


text: Sun Yat-sen University of Medical Scicences

text: live

text: Generic

text: Cardiac muscle

characteristic: cell type

text: The cDNA libraries were constructed using fetal heart in different growth stages and adult heart as materials, respectively. The cDNA probes-labelled from the total RNA of fetal heart hybridized with recombinant plasmid-denatured from the cDNA library of adult heart at the ratio of 1 to 10, which was repeated three times for enriched specific probes. Positive clones were screened by the way that the enriched specific probes hybridized with the given colonies of fetal heart cDNA library and sequenced by using T7 promoter primer.

text: embryonic day 96-140 post-fertilization

text: E.coli DH5a

text: heart

characteristic: organ

text: Homo sapiens

characteristic: organism

text: Human fetal heart cDNA library (Liu,L. and Gu,J.)

characteristic: title

text: Plasmid

text: pSPORT 1


text: The Pennsylvania State University

text: live

text: Generic

text: Agaricus bisporus

text: SalI

text: NotI

text: Basidiome and primordium cDNA libraries

text: Plasmid

text: pBluescript II SK (+)

characteristic: vector


text: Dana-Farber Cancer Institute

text: live

text: Generic

text: epithelial cells

text: Homo sapiens

text: female

characteristic: sex

text: breast

text: Immunomagnetically sorted breast epithelial cells (differential display)


text: Incyte Pharmaceuticals

text: live

text: Generic

text: multiple clone assembly from multiple libraries and donors

characteristic: description

text: adult

characteristic: dev-stage

text: Homo sapiens

text: prostate

text: Homo sapiens prostate adult


characteristic: External Id

text: Institut National de la Recherche Agronomique

text: live

text: Generic

text: Medicago truncatula

text: Medicago truncatula cDNA library


text: Biotechnology Research Center

text: live

text: Generic

characteristic: cultivar

text: Oryza sativa Indica Group

text: EcoRI

text: EcoRI

text: Oryza sativa cpslo17


text: C.N.R.S.

text: live

text: Generic

text: Torpedo marmorata

text: electric lobe

characteristic: tissue-type


text: Auburn University

characteristic: INSDC center name

text: live

text: Generic

text: Mature

text: Pituitary

text: Ictalurus punctatus

text: EcoRI

text: XhoI

text: Channel Catfish Lambda Unizap cDNA Library

text: Phage

text: Lambda vector Unizap II (Stratagene)


text: Institute of Molecular Biology and Genetics, Mahidol University, Salaya campus

characteristic: INSDC first public

text: live

text: Generic

text: The library was prepared using a kit and protocol given by a commercial supplier, Stratagene, Inc.

text: 4 months old average (farm-raised), multiple batches of shrimp

text: E.coli XL1-Blue MRF'

characteristic: lab-host

text: Penaeus monodon

text: EcoR I

text: Xho I

text: total hemolymph

text: Penaeus monodon total hemolymph cDNA library

text: Phage

text: lambdaZAP II


characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: cDNA made by oligo-dT priming. Non-directionally cloned into the UDG sites of pAMP10. Size-selected on agarose gel, average insert size 500 bp. Primary library; non-amplified. cDNA Library Preparation: David B. Krizman, Ph.D (NCI). Reference: Krizman et al. (1996) Cancer Research 56:5380-5383.

text: liver

text: Homo sapiens

text: plasmid (ampicillin resistant)


text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: lung

text: Homo sapiens

text: large cell carcinoma

text: plasmid (ampicillin resistant)


text: Immunex Corporation

text: live

text: Generic

text: Mus musculus

text: thymus

text: Mouse thymus


text: University of Nebraska

text: live

text: Generic

text: ESTs resulting from differential display experiment

text: follicular phase

text: ovary

characteristic: organ

text: Sus scrofa

text: female

text: follicle

text: Pig ovarian follicle cDNA


text: Facultad de Ciencias Quimicas, Universidad Nacional de Cordoba

text: live

text: Generic

text: Differential display products obtained by comparing normal and pathological gestational trophoblastic tissues were isolated, subcloned and sequenced using vector primer.

text: Homo sapiens

characteristic: organism

text: gestational trophoblastic tissues

text: Human differential display products


text: Philipps-University Marburg

text: live

text: Generic

text: SOLR

text: Guillardia theta

text: EcoRI

characteristic: strain

text: Guillardia theta Lambda Zap II cDNA Library

characteristic: title

text: Phage

text: Lambda ZAP II


text: University of Illinois

text: live

text: Generic

text: Williams

text: Library Gm-r1021 is a sequence-driven, reracked set of the original library Gm-c1004 which was prepared from root cDNA. The mRNA was isolated from entire roots of 8 day old 'Williams' seedlings which were propagated on paper towels with distilled water. Stratagene's cDNA Synthesis Kit (catalog #200401) was used to synthesize the cDNA. The Gm-c1004 library was constructed by Dr. Paul Keim & Virginia H. Coryell, Department of Biology, Box5640, Northern Arizona University, Flagstaff, AZ 86011, email: paul.keim@nau.edu, virginia.coryell@nau.edu. The contig analysis to select unique genes was performed by the laboratory of Ernest Retzel, Computational Biology Centers, University of Minnesota, http://www.cbc.umn.edu/ResearchProjects/Soybean/index.html. Reracking was performed by Genome Systems, St. Louis, http://www.genomesystems.com, and sequencing by the Keck Center for Comparative and Functional Genomics, University of Illinois, http://www.life.uiuc.edu/biotech/keck.html.

text: Glycine max

text: EcoRI

text: XhoI

text: root

text: pBluescript II XR

characteristic: vector


text: Institute of Basic Medical Sciences, University of Tsukuba

text: live

text: Generic

text: hematopoietic stem cell

characteristic: cell type

text: Mus musculus

text: mouse hematopoietic stem cell


text: Centre for Molecular Biology of Plants, University of Tuebingen

text: live

text: Generic

text: mRNA was isolated from suspension cell culture which was treated with the cytokinin BAP for 45 min, transcribed in double stranded cDNA and digested with Sau3A. After subtraction of an untreated cDNA pool the fragments where cloned in pUC19

characteristic: description

text: Nicotiana tabacum

text: suspension cell culture

text: Nicotiana tabacum L. cDNA

text: Plasmid


characteristic: External Id

text: Beijing Medical University

text: live

text: Generic

text: PC-3M high metastasis subline 1E8

characteristic: cell-line

text: Homo sapiens

text: prostate

text: Homo sapiens PC-3M high metastasis subline 1E8


text: Beijing Medical University

text: live

text: Generic

text: PC-3M low metastasis subline 2B4

text: Homo sapiens

text: prostate

characteristic: tissue-type

text: Homo sapiens PC-3M low metastasis subline 2B4


text: Ludwig Institute for Cancer Research

characteristic: INSDC center name

text: live

text: Generic

text: A mini-library was made by cloning products derived from ORESTES PCR (U.S. Letters Patent application No. 196,716 - Ludwig Institute for Cancer Research) profiles into the pUC 18 vector. Reverse transcription of tissue mRNA and cDNA amplification were performed under low stringency conditions.

text: Adult

characteristic: dev-stage

text: colon

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: Ludwig Institute for Cancer Research

characteristic: INSDC first public

text: live

text: Generic

text: Adult

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: Ludwig Institute for Cancer Research

characteristic: INSDC last update

text: live

text: Generic

text: Adult

text: stomach

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: National Institute of Neuroscience, NCNP

text: live

characteristic: INSDC status

text: Generic

text: CJ7 embryonic stem cell line

text: adult

text: Mus musculus

text: male

characteristic: sex

text: brain,liver,kidney,lung,heart,spleen

text: Mus musculus CJ7 brain, liver, kidney, lung, heart, spleen


text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: CJ7 embryonic stem cell line

text: adult

text: Mus musculus

text: male

text: brain,liver,kidney,heart

text: Mus musculus CJ7 brain, liver, kidney, heart


text: live

text: Generic

text: CJ7 embryonic stem cell line

text: adult

text: Mus musculus

characteristic: organism

text: male

text: brain, liver,kidney,lung,heart

text: Mus musculus CJ7 brain, liver, kidney, lung, heart


text: live

text: Generic

text: CJ7 embryonic stem cell line

text: adult

text: Mus musculus

text: male

characteristic: strain

text: liver,kidney,lung,heart

text: Mus musculus CJ7 liver, kidney, lung, heart


text: live

text: Generic

text: CJ7 embryonic stem cell line

text: adult

text: Mus musculus

text: male

text: brain,liver,lung,heart,spleen

text: Mus musculus CJ7 brain, liver, lung, heart, spleen

characteristic: title


text: live

text: Generic

text: CJ7 embryonic stem cell line

text: adult

text: Mus musculus

text: male

text: brain,liver,lung,heart

text: Mus musculus CJ7 brain, liver, lung, heart


text: Bio College Kyoto

text: live

text: Generic

text: seedling

text: Vigna angularis

text: epicotyl

text: Lambda gt 10 cDNA library, EVA01


text: National Cancer Center Research Institute

text: live

text: Generic

text: myeloid precursor

text: L-G

text: Mus musculus

text: Mus musculus L-G myeloid precursor


text: C.N.R.S.

text: live

text: Generic

text: Torpedo marmorata

text: electric lobe


text: live

text: Generic

text: Danio rerio

text: brain

text: Danio rerio brain


text: University of Delaware

text: live

text: Generic

text: E.coli DH10B

characteristic: lab-host

text: Gallus gallus

text: Male and Female

text: oviduct

text: chicken oviduct cDNA library

text: pCMV SPORT6

characteristic: vector


text: University of Delaware

text: live

text: Generic

text: E.coli DH10B

text: Gallus gallus

text: Male and Female

text: fat

text: chicken fat cDNA library pSPORT1


text: University of Delaware

text: live

text: Generic

text: E.coli DH10B

text: Gallus gallus

text: Male and Female

text: fat

text: chicken fat cDNA library pBSII

text: pBSII


text: National Cancer Institute-FCRDC

text: live

text: Generic

text: Felis catus

text: testis

text: Felis catus testis


text: University of Nebraska, Animal Science

text: live

text: Generic

text: ESTs resulting from differential display experiment

characteristic: description

text: 11 wks of age

text: Brain

characteristic: organ

text: Mus musculus

text: Male

text: Heat loss

text: Hypothalamus

text: Mouse hypothalamus cDNA


characteristic: External Id

text: live

text: Generic

text: Anchor enzyme: NlaIII~ This SAGE library is from Breast, isolated normal epithelial organoids. Derived from a reduction mammoplasty. 'Velculescu, V. E., Zhang, L., Vogelstein, B., and Kinzler, K. W. (1995) Serial Analysis of Gene Expression. Science 270, 484-487'

text: Homo sapiens

text: Breast


text: live

text: Generic

text: Anchor enzyme: NlaIII~ This SAGE library is from Breast, infiltrating ductal carcinoma of the breast. Stage II. Node negative. Estrogen and progesterone receptor positive. 'Velculescu, V. E., Zhang, L., Vogelstein, B., and Kinzler, K. W. (1995) Serial Analysis of Gene Expression. Science 270, 484-487'

text: Homo sapiens

text: Breast

characteristic: tissue-type


text: live

text: Generic

text: Anchor enzyme: NlaIII~ This SAGE library is from Ovary, ovarian cancer cell line A2780 'Velculescu, V. E., Zhang, L., Vogelstein, B., and Kinzler, K. W. (1995) Serial Analysis of Gene Expression. Science 270, 484-487'

text: Homo sapiens

text: Ovary


text: University of Nebraska, Animal Science

characteristic: INSDC center name

text: live

text: Generic

text: 11 wks of age

text: Brown Adipose Tissue (BAT)

text: Mus musculus

text: Male

text: Heat loss

text: Hypothalamus

text: Mouse Brown Adipose Tissue (BAT) cDNA


text: Ludwig Institute for Cancer Research

text: live

text: Generic

text: Adult

characteristic: dev-stage

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: Ludwig Institute for Cancer Research

characteristic: INSDC first public

text: live

text: Generic

text: Adult

text: breast

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: Ludwig Institute for Cancer Research

characteristic: INSDC last update

text: live

text: Generic

text: Adult

text: colon

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: Ludwig Institute for Cancer Research

text: live

characteristic: INSDC status

text: Generic

text: Adult

text: leiomios

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: Ludwig Institute for Cancer Research

text: live

text: Generic

text: Adult

text: pnet

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: Ludwig Institute for Cancer Research

text: live

text: Generic

text: Adult

text: stomach

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: Ludwig Institute for Cancer Research

text: live

text: Generic

text: Adult

text: testis

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: Washington University School of Medicine

text: live

text: Generic

text: Williams

characteristic: cultivar

text: This cDNA library was constructed from mRNA isolated from senecsing leave tissue of mature greenhouse grown plants. Complementary DNA was synthesized from mRNA using a 3' anchored poly(dT) primer. EcoRI adapters were ligated to the blunt-ended cDNA fragments followed by digestion with EcoRI and HindIII. The cDNA fragments were directionally cloned into the EcoRI-HindIII restriction site of the pT7T3-Pac vector. The ligated cDNA fragments were transformed into DH10B host cells (Gibco BRL). This library was constructed R. Shoemaker and J. Erpelding.

text: Glycine max

text: EcoRI

text: HindIII

text: Senescing leaves, mature plants, greenhouse grown.

text: plasmid

text: pT7T3-Pac (Pharmacia)


text: National Institute of Agrobiological Sciences

text: live

text: Generic

characteristic: NCBI submission model

text: BmN cultured cell

characteristic: cell type

text: BmNPV infected; 2 hr after inoculation

text: Bombyx mori

text: ovary

text: Bombyx mori ovary BmNPV infected; 2 hr after inoculation


text: live

text: Generic

characteristic: NCBI submission package

text: BmN cultured cell

text: BmNPV infected; 6 hr after inoculation

text: Bombyx mori

characteristic: organism

text: ovary

text: Bombyx mori ovary BmNPV infected; 6 hr after inoculation


text: live

text: Generic

text: BmN cultured cell

text: BmNPV infected; 12 hr after inoculation

text: Bombyx mori

text: ovary

text: Bombyx mori ovary BmNPV infected; 12 hr after inoculation

characteristic: title


text: live

text: Generic

text: P0 (just after pupation)

text: Bombyx mori

text: female/male mixed

characteristic: sex

text: Daizo

characteristic: strain

text: brain

text: Bombyx mori brain Daizo P0 (just after pupation)


text: live

text: Generic

text: Bombyx mori

text: female/male mixed

text: diapause-cancelled embryo

text: Bombyx mori diapause-cancelled embryo p50 76 hr after HCl treatment (HCl at 20 hr after oviposition)


text: live

text: Generic

text: hemocyte

text: spinning stage day-0

text: Bombyx mori

text: female/male mixed

text: Bombyx mori C108 spinning stage day-0


text: live

text: Generic

text: hemocyte

text: spinning stage day-3

text: Bombyx mori

text: female/male mixed

text: Bombyx mori C108 spinning stage day-3


text: live

text: Generic

text: donated by Dr. Sehnal, Czech

characteristic: description

text: 5th-instar larva

text: Bombyx mori

text: female/male mixed

text: middle silkgland

text: Bombyx mori middle silkgland 5th-instar larva


text: live

text: Generic

text: newly-eclosed adult

text: Bombyx mori

text: female

text: Shuko x Ryuhaku

text: pheromone gland

text: Bombyx mori pheromone gland Shuko x Ryuhaku newly-eclosed adult


text: live

text: Generic

text: 5th-instar day-4 larva

text: Bombyx mori

text: female/male mixed

text: prothoracic gland

text: Bombyx mori prothoracic gland p50 5th-instar day-4 larva


text: live

text: Generic

text: 5th-instar day-1 larva

text: Bombyx mori

text: female/male mixed

text: wing disk

text: Bombyx mori wing disk C108 5th-instar day-1 larva


text: live

text: Generic

text: 5th-instar day-3 larva

text: Bombyx mori

text: female/male mixed

text: wing disk

text: Bombyx mori wing disk C108 5th-instar day-3 larva


text: CNRS-LPR9042, Universite P. & M. Curie

text: live

text: Generic

text: The Laminaria gametophyte library, contructed by T Roscoe and F Crepineau, was oligo-(dT) primed and directionnally cloned into a Uni-ZAPTM XR vector (Stratagene, la Jolla, CA, USA) using total mRNA from gametophytes provided by R Kaas, IFREMER Nantes, France.

text: gametophyte

text: SOLR

characteristic: lab-host

text: Laminaria digitata

text: EcoRI

text: XhoI

text: L.digitata gametophyte Lambda ZapII

text: plasmid

text: pBluescript SK+

characteristic: vector


characteristic: External Id

text: CNRS-LPR9042, Universite P. & M. Curie

text: live

text: Generic

text: The Laminaria sporophyte library, contructed by T Roscoe and F Crepineau, was oligo-(dT) primed and directionnally cloned into a Uni-ZAPTM XR vector (Stratagene, la Jolla, CA, USA) using total mRNA from sporophytes harvested at l'ile de Sieck (F).

text: sporophyte

text: SOLR

text: Laminaria digitata

text: EcoRI

text: XhoI

text: L.digitata sporophyte Lambda ZapII

text: plasmid

text: pBluescript SK+


text: European Molecular Biology Laboratory

characteristic: INSDC center name

text: live

text: Generic

text: immune competent 4A3A

characteristic: cell-line

text: sequenced from forward priming site which reads from the 3' end of the cDNA. The 4A3A is a directionally cloned and normalized cDNA library that was constructed from the 4A3A cell line oligo-T primed cDNA according to: Bonaldo, Lennon & Soares (1996) : Normalization and Subtraction: Two approaches To Facilitate Gene Discovery, Genome Research 6, 791-806.

text: E. coli DH10B

text: Anopheles gambiae

text: EcoRI

text: NotI

text: 4A r/r

text: Anopheles gambiae immune competent 4A3A


text: European Molecular Biology Laboratory

characteristic: INSDC first public

text: live

text: Generic

text: immune competent 4A3B

text: sequenced from the forward priming site that reads from the 3' end of cDNA. The 4A3B is a directionally cloned and normalized cDNA library that was constructed from the 4A3B cell line oligo-T primed cDNA according to: Bonaldo, Lennon & Soares (1996): Normalization and Subtraction: Two Approaches To Facilitate Gene Discovery, Genome Research 6, 791-806.

text: E. coli DH10B

text: Anopheles gambiae

text: EcoRI

text: NotI

text: 4A r/r

text: Anopheles gambiae immune competent 4A3B


text: Peking Union Hospital

characteristic: INSDC last update

text: live

text: Generic

text: glioma cell

text: This fragment of cDNA was cloned from malignant glioma induced by EGF; using DDRT-PCR method and verified by northern blot

text: Homo sapiens

text: Human malignant glioma cell cDNA fragment


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

characteristic: INSDC status

text: Generic

text: cDNA made by oligo-dT priming. Directionally cloned into EcoRI/XhoI sites using the following 5' adaptor: GGCACGAG(G). Size-selected >500bp for average insert size 1.8kb. Library constructed by Ling Hong in the laboratory of Gerald M. Rubin (University of California, Berkeley) using ZAP-cDNA synthesis kit (Stratagene) and Superscript II RT (Life Technologies).

text: DH10B (phage-resistant)

text: muscle

characteristic: organ

text: Homo sapiens

text: EcoRI

text: XhoI

text: rhabdomyosarcoma

characteristic: tissue-type

text: plasmid (chloramphenicol resistant)


text: South African Institute For Medical Research

text: live

text: Generic

characteristic: NCBI submission model

text: Homo sapiens

text: Fetal brain

text: Fetal brain library

text: Plasmid


text: live

text: Generic

characteristic: NCBI submission package

text: Homo sapiens

text: Salivary Gland

text: Salivary Gland Library

text: Plasmid


text: Institute of Agricultural Science and Technology(NIAST)

text: live

text: Generic

text: ASI 2029

characteristic: cultivar

text: average insert size:1000 bp; initial pfu:5 X 10 7; Library information: Isolation of total RNA from the myceliaincubated in shaking liquid MCM media at 30 degrees C

text: shaking liquid cultured mycelia

characteristic: dev-stage

text: E.coli

text: Pleurotus ostreatus

characteristic: organism

text: EcoRI

text: XhoI

text: lambda Uni-ZAP XR


text: live

text: Generic

text: germinal center B cells

characteristic: cell type

text: Constructed from size fractionated cytoplasmic mRNA (3.5-4.4kb). Directionally cloned. Cells provided by Louis M. Staudt, Ph.D. Library preparation by Maria de Fatima Bonaldo, Ph.D. and M. Bento Soares, Ph.D.

text: DH10B (LTI)

text: Homo sapiens

text: NotI

text: Eco RI

text: lymph

characteristic: title

text: plasmid


text: Children's Memorial Research Center

text: live

text: Generic

text: germinal center B cells

text: Constructed from size fractionated cytoplasmic mRNA (4.4-7.4kb). Directionally cloned. Cells provided by Louis M. Staudt, Ph.D. Library preparation by Maria de Fatima Bonaldo, Ph.D. and M. Bento Soares, Ph.D.

characteristic: description

text: DH10B (LTI)

characteristic: lab-host

text: Homo sapiens

text: NotI

text: Eco RI

text: lymph

text: plasmid


characteristic: External Id

text: Children's Memorial Research Center

text: live

text: Generic

text: germinal center B cells

text: Constructed from size fractionated cytoplasmic mRNA (7.4-9.5kb). Directionally cloned. Cells provided by Louis M. Staudt, Ph.D. Library preparation by Maria de Fatima Bonaldo, Ph.D. and M. Bento Soares, Ph.D.

text: DH10B (LTI)

text: Homo sapiens

text: NotI

text: Eco RI

text: lymph

text: plasmid

characteristic: vector


characteristic: INSDC center name

text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Size-selected >1.5 kb for average insert size 2 kb. Primary library; non-amplified. This library was constructed by M. Mione (University College London, Dept of Anatomy and Developmental Biology).

text: 13.5 dpc

text: brain

text: Mus musculus

text: NotI

text: SalI

text: mixed

characteristic: sex

text: forebrain

text: Mione mouse WTB

text: phagemid (ampicillin resistant)


characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: cDNA made by oligo-dT priming. Non-directionally cloned into the UDG sites of pAMP10. Size-selected on agarose gel, average insert size 500 bp. Primary library; non-amplified. cDNA Library Preparation: David B. Krizman, Ph.D (NCI). Reference: Krizman et al. (1996) Cancer Research 56:5380-5383.

text: lung

text: Homo sapiens

text: small cell carcinoma

text: plasmid (ampicillin resistant)


text: Institute of Molecular Biology and Genetics, Mahidol University, Salaya campus

text: live

characteristic: INSDC status

text: Generic

text: The library was prepared using protocol given by supplier, Stratagene, Inc.

text: 2 months old average (farm-raised)

text: E.coli XL-1-Blue MRF'

text: Penaeus monodon

text: EcoRI

text: XhoI

text: total abdominal muscles

text: Penaeus monodon's abdominal cDNA library

text: Phage

text: lambdaZAP II


text: Washington University School of Medicine

text: live

text: Generic

text: Williams

text: This cDNA library was constructed from mRNA isolated from very young cotyledons (20-50mgs fresh weight) of greenhouse grown plants. The library was prepared using the Life Technologies pSuperScript cDNA library construction kit. Complementary DNA was synthesized from mRNA using a poly(dT) sequence with a Not I restriction site. Sal I linker adapters were ligated to the blunt-ended cDNA fragments followed by Not I digestion. The cDNA fragments were directionally cloned into the Not I-Sal I restriction site of the pSPORT1 vector. The ligated cDNA fragments were transformed into E.coli Electromax DH10B host cells. This library was constructed by Dr. Paul Keim and Dr. Virginia Coryell.

text: Glycine max

text: Not I

text: Sal I

text: very young cotyledons of greenhouse grown plants

characteristic: tissue-type

text: plasmid


text: live

text: Generic

characteristic: NCBI submission model

characteristic: cell-line

text: Anchor enzyme: NlaIII~ Cystadenoma line ML10

text: adult

text: ovary

characteristic: organ

text: Homo sapiens

text: female

text: cystadenoma

text: plasmid


text: live

text: Generic

characteristic: NCBI submission package

text: Anchor enzyme: NlaIII~ Cell line grown under normal atmospheric conditions in zinc option media with 10% RBS and harvested at passage 102. Library constructed in the laboratory of G. Riggins, M.D., Ph.D. (Duke University).

text: brain

text: Homo sapiens

text: glioblastoma multiforme

text: plasmid


text: live

text: Generic

text: Anchor enzyme: NlaIII~ Cell line grown under 1.5% oxygen conditions for 24 hours prior to harvesting in zinc option media with 10% RBS and harvested at passage 102. Library constructed in the laboratory of G. Riggins, M.D., Ph.D. (Duke University).

text: brain

text: Homo sapiens

characteristic: organism

text: glioblastoma multiforme

text: plasmid


text: live

text: Generic

text: Oligo (dT) primed library. cDNA was contructed and cloned unidirectionally into the vector within the 5' EcoRI and 3' XhoI sites. This library was constructed by Dr. Uma Rao and Dr. David Bird at North Carolina state University.

text: enriched for 2nd stage juveniles

characteristic: dev-stage

text: XLORL

text: Meloidogyne incognita

text: EcoRI

text: XhoI

text: Bird-Rao Meloidogyne incognita J2

text: phagemid (Kanamycin resistant)

text: ZAP express - pBKCMV (Stratagene)


text: Institution of Medical Biology, CAMS

text: live

text: Generic

text: liver

text: Homo sapiens

text: FLE

characteristic: title


text: Cardiovascular Institute, CAMS & PUMC

text: live

text: Generic

text: The rat stroke model was developed by Wang Xianmei, Zhu Zhiming, Zhu Shanjun et al at 3rd Military Medical University Chongqing, China. The Suppression Subtraction Hybridization (SSH) was conducted at Fu Wai Hospital by Zhao Bin and Wang Xianmei in Beijing, China. Message RNA was extracted from pooled whole cerebrum from stroke and control rats respectively. SSH had been conducted following manufacture's manual using 5ug mRNA. The SSH library was constructed by Zhao Bin and Wang Xianmei using pMD18-T vector. Randomly picked clones were sequenced at ABI 377 using M13R primer (AGCGGATAACAATTTCACACAGG) by Wang Xianmei, Zhao Bin and Zhang Qian.

characteristic: description

text: 8-12 weeks old

text: Rattus norvegicus

text: male

text: Wistar

characteristic: strain

text: Cerebrum

text: Plasmid Subtractive Library of Rat Cerebrum

text: Plasmid (T vector)

text: pMD-T (TakaRa, D504 CA)


characteristic: External Id

text: Otto-von-Guericke University Magdeburg

text: live

text: Generic

text: HaCaT-keratinocytes

characteristic: cell type

text: Homo sapiens

text: Homo sapiens HaCaT-keratinocytes


text: IRBV, Univ. of Montreal

characteristic: INSDC center name

text: live

text: Generic

text: induced by drought

text: Oryza sativa

text: panicle

text: Oryza sativa induced by drought


text: Fourth Military Medical University

text: live

text: Generic

text: hypophyses of cold-stress SD rats that exposed in -10 centigrade for 4 hours; mRNA differential display PCR

text: Rattus norvegicus

text: SD

text: hypophyses of cold-stress SD rats


text: The Pennsylvania State University

text: live

text: Generic

text: Agaricus bisporus

text: SalI

text: NotI

text: Basidiome

text: Basidiome cDNA library

text: Plasmid

text: pBluescript II SK (+)

characteristic: vector


text: The Pennsylvania State University

characteristic: INSDC first public

text: live

text: Generic

text: Agaricus bisporus

text: SalI

text: NotI

text: Primordium

text: Primordium cDNA library

text: Plasmid

text: pBluescript II SK (+)


text: C.S.I.R.O.

characteristic: INSDC last update

text: live

text: Generic

text: endosperm

text: Wyuna

characteristic: cultivar

text: Triticum aestivum

text: BRY


text: Pennsylvania State University College of Medicine

text: live

characteristic: INSDC status

text: Generic

text: ATCC HTB-12; SW1088

text: Homo sapiens

text: astrocytoma

characteristic: tissue-type

text: Homo sapiens ATCC HTB-12; SW1088


text: University of Newcastle upon Tyne

text: live

text: Generic

characteristic: NCBI submission model

text: Danio rerio

text: brain

text: pZIPLOX Danio rerio brain

text: pZIPLOX


text: Xijing Hospital

text: live

text: Generic

characteristic: cell-line

text: Homo sapiens

text: prostate tumor

text: Homo sapiens PC-3 prostate tumor


text: Copenhagen University Hospital

text: live

text: Generic

characteristic: NCBI submission package

text: The EST is derived from direct sequencing of a Differential Display fragment. Laboratory manuals are available from http//www.biobase.dk/~ddbase

text: Homo sapiens

text: Neuronal Differentiation of the NT2/D1 cell line.


text: Institute of Radiation Medicine, Beijing

text: live

text: Generic

text: kidney

characteristic: organ

text: Homo sapiens

characteristic: organism

text: EcoRI

text: XbaI

text: fetal human kidney lambda Triplex library

text: plasmid

text: pTriplex


text: live

text: Generic

text: The NIH_BMAP_M_S4 library is a subtracted library of a series, ultimately derived from a mixture of individually tagged normalized libraries from ten regions of the mouse brain (cerebellum, brain stems, olfactory bulbs, hypothalamus, cortex, amygdala, basal ganglia, pineal gland, striatum, hipoccampus) after a series of subtractions to reduce the representation of cDNAs from which ESTs had already been generated. The following serially subtracted libraries were generated in this process: NIH_BMAP_M_S4, NIH_BMAP_M_S3.3, NIH_BMAP_M_S3.2, NIH_BMAP_M_S3.1, NIH_BMAP_M_S2, NIH_BMAP_M_S1. The subtracted library (NIH_BMAP_M_S4) was constructed as follows: PCRamplified cDNA inserts from NIH_BMAP_M_S3.3, NIH_BMAP_M_S3.2, and NIH_BMAP_M_S3.1 clones from which 3' ESTs had been derived was used as a driver in a hybridization with a pool of the NIH_BMAP_M_S3.3, NIH_BMAP_M_S3.2, and NIH_BMAP_M_S3.1 libraries in the form of single-stranded circles. The remaining single-stranded circles (subtracted library) was purified by hydroxyapatite column chromatography, converted to double-stranded circles and electroporated into DH10B bacteria (LifeTechnologies) to generate the NIH_BMAP_M_S4 library. This procedure has been previously described (Bonaldo, Lennon and Soares, Genome Research 6:791-806, 1996)

text: 27-32 days

characteristic: dev-stage

text: DH10B (Life Technologies)

characteristic: lab-host

text: Mus musculus

text: Not I

text: Eco RI

characteristic: title

text: Phagemid


characteristic: External Id

text: University of Illinois at Urbana-Champaign

text: live

text: Generic

text: The cDNA library was contributed by the Soares laboratory and it was constructed and normalized as described by Bonaldo, M.F., Lennon, G. and Soares, M.B. (1996), Genome Research 6(9): 791-806.

characteristic: description

text: placenta

text: Bos taurus

text: EcoRI

text: NotI

text: female

characteristic: sex

text: Soares normalized bovine placenta

text: Plasmid


text: University of Missouri

characteristic: INSDC center name

text: live

text: Generic

text: Abundant cDNAs were removed from the initial unnormalized cDNA library by subtraction with use of an abundant cDNA pool. 'Rare' cDNAs are in pBluescript SK(-)

text: dauer larva

text: Caenorhabditis elegans

text: hermaphrodite

text: Bristol N2

characteristic: strain

text: 'Rare' cDNA library from Caenorhabditis elegans

text: pBluescript SK(-)

characteristic: vector


text: Iowa State University

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: This cDNA library was constructed from mRNA isolated from immature cotyledons of greenhouse grown plants (individual seed fresh weight of 100-300mg). The library was prepared using the Life Technologies pSuperScript cDNA library construction kit. Complementary DNA was synthesized from mRNA using a poly(dT) sequence with a NotI restriction site. SalI linkers adapters were ligated to the blunt-ended cDNA fragments followed by NotI digestion. The cDNA fragments were directionally cloned into the NotI-SalI restriction site of the pSPORT1 vector. The ligated cDNA fragments were transformed into E. coli ElectroMax DH10B host cells. This library was constructed by Dr. Lila Vodkin and Dr. Anu Khanna. Note that Gm-r1030 is a re-rack of Gm-c1007.

text: Glycine max

text: SalI

text: NotI

text: plasmid


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

characteristic: INSDC status

text: Generic

text: 1st strand cDNA was prepared from mRNA obtained from pooled lung tumors with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCCTCTGTTTTTTTTTTTTTTTTT 3']. Double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library went through one round of normalization, and was constructed by Bento Soares and M. Fatima Bonaldo.

text: DH10B (phage-resistant)

text: lung

text: Mus musculus

text: NotI

text: EcoRI

text: CZECH II

text: pooled lung tumors

characteristic: tissue-type

text: phagemid (ampicillin resistant)


text: live

text: Generic

characteristic: NCBI submission model

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCCTGGTTTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library is normalized; constructed by Bento Soares and M.Fatima Bonaldo.

text: DH10B (phage-resistant)

text: germinal B-cell

text: Mus musculus

text: Not I

text: Eco RI

text: phagemid (ampicillin resistant)


text: University of Massachusetts Medical School

text: live

text: Generic

characteristic: NCBI submission package

text: Late gastrula (8hpf)

text: Danio rerio

text: Zebrafish gastrula posterior minus anterior subtracted library


text: GSF - Forschungszentrum, Institut fuer Molekulare Strahlenbiologie

text: live

text: Generic

text: bursal lymphocyte

characteristic: cell type

text: 2-3 weeks old

text: Gallus gallus

text: CB

text: Bursa of Fabricius


text: Osaka University

text: live

text: Generic

text: Oryza sativa

characteristic: organism

text: Oryza sativa library (Watanabe T)


text: University of Virginia

text: live

text: Generic

text: Crackerjack

characteristic: cultivar

text: isolated by differential display

text: Tagetes erecta

text: Tagetes erecta Crackerjack


text: Beijing Medical University

text: live

text: Generic

text: PC-3M subline IE8

characteristic: cell-line

text: Homo sapiens

text: prostate cancer

text: Homo sapiens PC-3M subline IE8


text: University of Virginia, Health Sciences System

text: live

text: Generic

text: Stratagene library (catalogue #937227)

text: Fetal 19 - 23 weeks

characteristic: dev-stage

text: XL1-Blue MRF'

characteristic: lab-host

text: Fetal brain

characteristic: organ

text: Homo sapiens

text: EcoR I

text: Xho I

text: Female/male

text: Human Fetal Brain cDNA in Uni-ZAP XR Vector

characteristic: title

text: Phage

text: Uni-ZAP XR


characteristic: External Id

text: C.N.R.S.

characteristic: INSDC center name

text: live

text: Generic

text: Torpedo marmorata

text: electric lobe

text: Torpedo marmorata electric lobe


text: C.N.R.S.

text: live

text: Generic

text: Torpedo marmorata

text: electric organ

text: Torpedo marmorata electric organ


text: Bernhard Nocht Institute for Tropical Medicine

text: live

text: Generic

text: Filarial nematode parasite of humans. Three adult female worms of Onchocerca volvulus were isolated from one consenting patient from western Uganda. The patient was treated 26 hours before nodulectomy with a single dose of 150 mg/kg ivermectin. Adult female worms were quick frozen. Their mRNA was converted to double-stranded cDNA using reverse transcriptase and oligo(dT) followed by RNase H and DNA pol I. The library has 6 x 10E4 independent recombinants and the average insert size is ~920bp. The library was constructed by Peter Fischer with worms provided by Dr. Dietrich W. Buttner. The library is available from Dr. Peter Fischer (Germany), Pfischer@bni.uni-hamburg.de

characteristic: description

text: adult

text: XL1-Blue MRF'

text: Onchocerca volvulus

text: Eco RI

text: Xho I

text: female

characteristic: sex

text: O. volvulus adult female cDNA 26 h following ivermectin (PF99PF-OvAF26)

text: Phage

text: Lambda Uni-ZAP XR

characteristic: vector


text: Scottish Crop Research Institute

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: mRNA extracted from preparasitc J2s of Globodera rostochiensis cloned directionally into pcDNAII plasmid vector.

text: Second stage juvenile

text: Globodera rostochiensis

text: EcoRI

text: NotI

text: Globodera rostochiensis J2 pcDNAII

text: plasmid

text: pcDNAII (Invitrogen)


text: Scottish Crop Research Institute

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: mRNA extracted from preparasitc J2s and adult (gravid) females of Globodera pallida cloned into lambda gt11 vector.

text: Second stage juvenile & adult female

text: Globodera pallida

text: EcoRI

text: EcoRI

text: Globodera pallida gt11

text: phage

text: lambda gt11


text: Children's Memorial Research Center

text: live

text: Generic

text: The UI-R-BJ1 library is a subtracted library derived from the following tissues: atrium at 16.5 dpc, ventricle at 16.5 dpc, AV canal at 16.5 dpc, atrium at 15 dpc, ventricle at 15 dpc, AV canal at 15 dpc, ventricle at 13 dpc, and adult heart. For a detailed description of the library from which this clone was derived, please visit our web site at ratest.eng.uiowa.edu. The subtraction has been previously described in (Bonaldo, Lennon and Soares, Genome Research 6:791-806, 1996)

text: DH10B (Life Technologies)

text: Rattus norvegicus

characteristic: organism

text: Not I

text: Eco RI

text: Sprague-Dawley

characteristic: strain

text: Phagemid


text: Children's Memorial Research Center

text: live

text: Generic

text: The UI-R-BX0 library is derived from 13 dpc whole embryo tissue. For a detailed description of the library from which this clone was derived, please visit our web site at ratest.eng.uiowa.edu.

text: embryonic 13 dpc

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid


text: Children's Memorial Research Center

text: live

text: Generic

text: The UI-R-BS0 library is derived from 13 dpc whole embryo tissue. For a detailed description of the library from which this clone was derived, please visit our web site at ratest.eng.uiowa.edu.

text: embryonic 13 dpc

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid


text: Children's Memorial Research Center

text: live

text: Generic

text: The UI-R-BV0p library is derived from 13 dpc whole embryo tissue. For a detailed description of the library from which this clone was derived, please visit our web site at ratest.eng.uiowa.edu.

text: embryonic 13 dpc

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid


text: Children's Memorial Research Center

text: live

text: Generic

text: The library UI-R-BT1 is a subtracted library derived from a mixture of the following tissues: hippocampus, thalamus, mid-brain, medulla, corpus striatum, cerebral cortex and testis. For a detailed description of the library from which this clone was derived, please visit our web site at ratest.eng.uiowa.edu. The subtraction has been previously described in (Bonaldo, Lennon and Soares, Genome Research 6:791-806, 1996)

text: adult

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid


text: Children's Memorial Research Center

text: live

text: Generic

text: The UI-R-BU0 library is a subtracted library derived from a mixture of eye and ganglia tissues. For a detailed description of the library from which this clone was derived, please visit our web site at ratest.eng.uiowa.edu. The subtraction has been previously described in (Bonaldo, Lennon and Soares, Genome Research 6:791-806, 1996)

text: adult

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid


text: Children's Memorial Research Center

text: live

text: Generic

text: The UI-R-C4 library is a subtracted library of a series, ultimately derived from a mixture of tissues from rat placenta, adult lung, brain, liver, kidney, heart, spleen, ovary, muscle, and 8, 12 and 18-day embryos. For a detailed description of the library from which this clone was derived, please visit our web site at ratest.eng.uiowa.edu. This procedure has been previously described (Bonaldo, Lennon and Soares, Genome Research 6:791-806, 1996)

text: adult

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid


text: Children's Memorial Research Center

text: live

text: Generic

text: The library (UI-R-BO1) is a subtracted library derived from a mixture of the following tissues: thalamus, cerebellum, hypothalamus, medulla, pons, midbrain, cerebral cortex, corpus striatum and hippocampus. For a detailed description of the library from which this clone was derived, please visit our web site at ratest.eng.uiowa.edu. The subtraction has been previously described in (Bonaldo, Lennon and Soares, Genome Research 6:791-806, 1996)

text: adult

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid


text: Children's Memorial Research Center

text: live

text: Generic

text: The library (UI-R-BO0) is a subtracted library derived from a mixture of the following tissues: thalamus, cerebellum, hypothalamus, medulla, pons, midbrain, cerebral cortex, corpus striatum and hippocampus. For a detailed description of the library from which this clone was derived, please visit our web site at ratest.eng.uiowa.edu. The subtraction has been previously described in (Bonaldo, Lennon and Soares, Genome Research 6:791-806, 1996)

text: adult

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid


text: Naval American Medical Research Unit#3 (NAMRU-3)

text: live

text: Generic

text: Adult worm

text: Schistosoma haematobium

text: EcoRI

text: NotI

text: Mixed

text: Egyptian

text: Schistosoma haematobium Lambda gt22A cDNA Library

text: Phage

text: Lambda gt22A


text: Cardiovascular Institute, CAMS & PUMC

text: live

text: Generic

text: The rat stroke model was developed by Wang Xianmei, Zhu Zhiming, Zhu Shanjun et al at 3rd Military Medical University Chongqing, China. The Suppression Subtraction Hybridization (SSH) was conducted at Fu Wai Hospital by Zhao Bin and Wang Xianmei in Beijing, China. Message RNA was extracted from pooled whole cerebrum from stroke and control rats respectively. SSH had been conducted following manufacture's manual using 5ug mRNA. The SSH library was constructed by Zhao Bin and Wang Xianmei using pMD18-T vector. Randomly picked clones were sequenced at ABI 377 using M13R primer(AGCGGATAACAATTTCACACAGG) by Wang Xianmei, Zhao Bin and Zhang Qian.

text: 8-12 weeks old

text: Rattus norvegicus

text: male

text: Wistar

text: Cerebrum

characteristic: tissue-type

text: Plasmid Subtractive Library of Rat Cerebrum (stroke)

text: Plasmid (T vector)

text: pMD-T (TakaRa, D504 CA)


text: Cardiovascular Institute, CAMS & PUMC

text: live

text: Generic

text: 8-12 weeks old

characteristic: dev-stage

text: Rattus norvegicus

text: male

text: Wistar

text: Cerebrum

text: Plasmid Subtractive Library of Rat Cerebrum (control)

text: Plasmid (T vector)

text: pMD-T (TakaRa, D504 CA)


text: The Institute for Genomic Research

text: live

text: Generic

text: Clontech cerebral cortex; No M13 primer sites, cat# HL1143x

text: 15 years

text: brain

text: Homo sapiens

text: BamHI

text: XbaI

text: F

text: cerebral cortex

text: brain III, cerebral cortex


text: The Institute for Genomic Research

text: live

text: Generic

text: Homo sapiens

text: HindIII

text: NotI

text: B lymphocytes

text: brain I, Batten's subtracted

text: pBluescript SK-


text: Beijing Institute of Toxicology and Pharmacology

text: live

text: Generic

text: pheochromocytoma cell line

characteristic: cell-line

text: cerebrolysin treated (PC12 cells) for 6 days

text: adrenal gland

text: Rattus norvegicus

text: male

text: Sprague-Dawley

text: Rat PC12 cells


text: Institute of Plant Physiology of Shanghai Institutes for Biological Sciences, The Chinese Acadamy of Sciences

text: live

text: Generic

characteristic: cultivar

text: -3 to 15 days post anthesis

text: Ovule

characteristic: organ

text: Gossypium hirsutum

text: Cotton fiber and embryo Lambda Zap Express Library

text: Phage


characteristic: External Id

text: International Centre for Genetic Engineering and Biotechnology

text: live

text: Generic

text: Pusa bold

text: Brassica juncea

text: Brassica juncea Pusa bold

characteristic: title


text: National Institute on Aging/National Institutes of Health

characteristic: INSDC center name

text: live

text: Generic

text: Total RNAs were extracted from 6 Embryo. The double-stranded cDNA was synthesized by Gibco's kit with an Oligo(dT) primer [NotI primer-adapter from GibcoBRL] [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 0.51ug of mRNA . The double-stranded cDNAs were treated with T4 DNA polymerase and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal3 (include Sal1 sequence). The cDNAs were purified by phenol/chloroform and separated from free linkers by Centricon 100. Then, cDNAs were amplified by long-range high fidelity PCR using Takara's Ex Taq polymerase. Then, the cDNAs were purified by phenol/chloroform and by Centricon 100. The cDNAs were digested with SalI and NotI enzymes. Then, the cDNAs were size selected by Gibco's Size Fractionation Column. The cDNAs were cloned into SalI/NotI site of pSPORT1 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by chemical method. The library was constructed by Xiaohong Wang and Minoru S. H. Ko.

characteristic: description

text: 7.5dpc Embryo

characteristic: lab-host

text: Mus musculus

text: SalI

text: NotI

text: NIA Mouse E7.5 Embryonic Portion cDNA Library

text: plasmid (ampicillin resistant)

text: pSPORT1 (Gibco/BRL Life Technology)


characteristic: INSDC first public

text: live

text: Generic

text: Total RNAs were extracted from 2 Mesonephros. The double-stranded cDNA was synthesized by Gibco's kit with an Oligo(dT) primer [NotI primer-adapter from GibcoBRL] [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 3.42ug of total RNA . The double-stranded cDNAs were treated with T4 DNA polymerase and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal3 (include Sal1 sequence). The cDNAs were purified by phenol/chloroform and separated from free linkers by Centricon 100. Then, cDNAs were amplified by long-range high fidelity PCR using Takara's Ex Taq polymerase. Then, the cDNAs were purified by phenol/chloroform and by Centricon 100. The cDNAs were digested with SalI and NotI enzymes. Then, the cDNAs were size selected by Gibco's Size Fractionation Column. The cDNAs were cloned into SalI/NotI site of pSPORT1 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by chemical method. The library was constructed by Xiaohong Wang.

text: Mus musculus

text: SalI

text: NotI

text: female

characteristic: sex

text: NIA Mouse E12.5 Female Mesonephros and Gonads cDNA Library

text: plasmid (ampicillin resistant)

text: pSPORT1 (Gibco/BRL Life Technology)

characteristic: vector


characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Total RNAs were extracted from 7 Newborn Ovary. The double-stranded cDNA was synthesized by Gibco's kit with an Oligo(dT) primer [NotI primer-adapter from GibcoBRL] [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 2.56ug of total RNA . The double-stranded cDNAs were treated with T4 DNA polymerase and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal3 (include Sal1 sequence). The cDNAs were purified by phenol/chloroform and separated from free linkers by Centricon 100. Then, cDNAs were amplified by long-range high fidelity PCR using Takara's Ex Taq polymerase. Then, the cDNAs were purified by phenol/chloroform and by Centricon 100. The cDNAs were digested with SalI and NotI enzymes. Then, the cDNAs were size selected by Gibco's Size Fractionation Column. The cDNAs were cloned into SalI/NotI site of pSPORT1 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by chemical method. The library was constructed by Xiaohong Wang and Yulan Piao.

text: Newborn Ovary

text: Mus musculus

text: SalI

text: NotI

text: female

text: NIA Mouse Newborn Ovary cDNA Library

text: plasmid (ampicillin resistant)

text: pSPORT1 (Gibco/BRL Life Technology)


text: live

text: Generic

characteristic: NCBI submission package

text: Total RNAs were extracted from 5 EPC. The double-stranded cDNA was synthesized by Gibco's kit with an Oligo(dT) primer [NotI primer-adapter from GibcoBRL] [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 0.8ug of mRNA . The double-stranded cDNAs were treated with T4 DNA polymerase and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal3 (include Sal1 sequence). The cDNAs were purified by phenol/chloroform and separated from free linkers by Centricon 100. Then, cDNAs were amplified by long-range high fidelity PCR using Takara's Ex Taq polymerase. Then, the cDNAs were purified by phenol/chloroform and by Centricon 100. The cDNAs were digested with SalI and NotI enzymes. Then, the cDNAs were size selected by Gibco's Size Fractionation Column. The cDNAs were cloned into SalI/NotI site of pSPORT1 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by chemical method. The library was constructed by Xiaohong Wang.

text: 7.5dpc Embryo

text: Mus musculus

characteristic: organism

text: SalI

text: NotI

characteristic: strain

text: NIA Mouse E7.5 Extraembryonic Portion cDNA Library

text: plasmid (ampicillin resistant)

text: pSPORT1 (Gibco/BRL Life Technology)


text: Glostrup Hospital, University of Copenhagen

text: live

text: Generic

text: skeletal muscle

characteristic: cell type

text: cDNA that is differentially upregulated in extraocular muscle compared to skeletal muscle

text: 3 month old

characteristic: dev-stage

text: Rattus norvegicus

text: female

text: Wistar-BR

text: extraocular muscle

characteristic: tissue-type

text: Rat differential display Library


characteristic: External Id

text: Friedrich-Alexander-Universitaet Erlangen-Nuernberg

text: live

text: Generic

text: T lymphocyte

characteristic: cell-line

text: Homo sapiens

text: Homo sapiens HTLV-1 positive adult T-cell leukemia cell line JuanaW


text: Academic Medical Center, University of Amsterdam

characteristic: INSDC center name

text: live

text: Generic

text: vascular smooth muscle

text: Human vascular smooth muscle cells were activated for 0, 1, 2, 4, 8 or 24h with the conditioned medium of ox-LDL stimulated macrophages. Total RNA was isolated and subjected to Differential Display/RT-PCR according to Liang, P. & Pardee, A.B. (1992, Science 257:967-971) with 12 random decamers as described by Bauer, D. et al., (1993, Nucleic Acids Res. 21:4272-80). Differential bands were recovered from the gel and subcloned in TA-cloning plasmids.

text: Umbilical cord

characteristic: organ

text: Homo sapiens

text: Umbilical cord artery

text: Human (activated) smooth muscle cells, DD/RT-PCR products

characteristic: title

text: Plasmid


text: The Rockefeller University

characteristic: INSDC first public

text: live

text: Generic

text: macrophage

text: Clontech PCR-Select cDNA Subtraction for cAMP induced genes from RAW264 cells, confirmed by northern blot analysis.

characteristic: description

text: Mus musculus

text: cAMP inducible RAW264 cell

text: pT-Adv


text: live

text: Generic

text: Epithelium

text: Anchor enzyme: NlaIII~ Tissue supplier: LNCaP cells from ATCC~Library producer: Chen Laboratory~LNCaP cells were cultured in the absence of androgens for 3 months, and then in the presence of 1 nM of dihydrotestosterone for 24 hours.

text: Prostate

text: Homo sapiens

text: NlaIII

text: Male

characteristic: sex

text: Prostate carcinoma cell line


characteristic: INSDC last update

text: live

text: Generic

text: Epithelium

text: LNCaP

text: Anchor enzyme: NlaIII~ Tissue supplier: LNCaP cells from ATCC~Library producer: Chen Laboratory~LNCaP cells were cultured in the absence of androgens for 3 months.

text: Prostate

text: Homo sapiens

text: NlaIII

text: Male

text: Prostate carcinoma cell line


text: live

characteristic: INSDC status

text: Generic

text: Epithelium and stroma

text: Anchor enzyme: NlaIII~ Library producer: Chen Laboratory~Tissues were pooled from 4 patients. The samples contained 30-50% normal glands and 50-70% stroma with no tumor glands. None of the patients had any hormonal, radiation or chemotherapy for prostate cancer prior to surgery.

text: Prostate

text: Homo sapiens

text: NlaIII

text: Male

text: Normal prostate tissue


text: live

text: Generic

characteristic: NCBI submission model

text: Epithelium and stroma

text: Anchor enzyme: NlaIII~ Tissues were pooled from 4 patients. The samples contained 80-90% malignant glands (Gleason score 3 and 4) and 10-20% stroma with no normal glands. None of the patients had any hormonal, radiation or chemotherapy for prostate cancer prior to surgery.

text: Prostate

text: Homo sapiens

text: NlaIII

text: Male

text: Prostate tumor tissue


text: Beijing Institute of Radiation Medicine

text: live

text: Generic

characteristic: NCBI submission package

text: peripheral blood leukocytes

text: pooled from 6hr & 24hr PHA stimulated peripheral blood leukocytes

text: Homo sapiens

text: male

text: Caucasian

text: Human leukocyte MATCHMAKER cDNA Library


text: Kazusa DNA Research Institute

text: live

text: Generic

text: two to six-week old plants

text: Arabidopsis thaliana

characteristic: organism

text: EcoRI

text: XhoI

text: Columbia

text: aboveground organ

text: Arabidopsis thaliana Above-ground organ from two to six-week old plants Columbia

text: pBluescriptII SK-

characteristic: vector


text: Ludwig Institute of Cancer Research

text: live

text: Generic

text: fibroblast

characteristic: cell type

text: sodium salicylate and/or TNF-treated

text: embryo

text: Mus musculus

characteristic: strain

text: Mus musculus sodium salicylate and/or TNF-treated


text: Beijing Institute of Radiation Medicine

text: live

text: Generic

text: ages 20-25 weeks

characteristic: dev-stage

text: Fetal Brain

text: Homo sapiens

text: male/female

text: Human Fetal Brain MATCHMAKER cDNA Library


characteristic: External Id

text: Tufts University School of Veterinary Medicine

text: live

text: Generic

text: synonym: Litopenaeus vannamei; Obtained by mRNA differential display following the RNAimage protocol (GenHunter Corporation, Nashville, TN). cDNA was synthesized using one base anchored oligo-d(T) primer followed by the PCR reaction in the presence of a second arbitrary primer. Selected bands were cloned into pCR-TRAP vector.

text: Juvenile

text: GH K12

characteristic: lab-host

text: Penaeus vannamei

text: Hind III

text: Whole shrimp (~1.5 g)

characteristic: tissue-type

text: Shrimp challenged with Taura Syndrome Virus (TSV)

text: Plasmid

text: pCR-TRAP (GenHunter Corp)


text: Washington University School of Medicine

characteristic: INSDC center name

text: live

text: Generic

text: Williams

characteristic: cultivar

text: This cDNA library was constructed from mRNA isolated from whole 'Williams' seedlings, minus the cotyledons, which were propagated on paper towels with distilled water for 5 days, incubated at 40 degrees C for 1 hour. The cotyledons were removed and the remaining tissue was flash-frozen in liquid nitrogen. Stratagene's cDNA Synthesis Kit (catalog number 200401) was used to synthesize the cDNA. First-strand synthesis was performed with 5-methyl dCTP, hence the ligated cDNA was hemimethylated. A modification of Stratagene's first-strand synthesis primer was used. An anchor nucleotide (V=A, C, or G) was added to the 3' end of the primer [GAGAGAGAGAGAGAGAGAGAACTAGTCTCGAG(T)18V] to anchor the primer at the 5' end of the poly(A) tract. After second strand synthesis, the cDNA ends were filled in with cloned Pfu DNA, ligated to EcoRI adapters and subsequently phosphorylated. The cDNA was then precipitated and redissolved in sterile, RNase-, DNase-free water. The XhoI site within the first-strand synthesis primer was then restricted by digestion with XhoI from Promega (40U/ul); all XhoI sites in the cDNA would be protected by their hemimethylated status. The cDNA constructs were size-fractionated with a 500 bp cutoff, using GibcoBRL Life Technologies' cDNA Size Fractionation column. The column eluent was then precipitated, redissolved, and ligated into Stratagene's pBluescript II XR Predigested vector (pBluescript II SK(+) vector that has been digested with EcoRI and XhoI, and phosphorylated by Stratagene). 100% of the white and blue colonies appear to contain recombinant plasmids with cDNA inserts, based on size (n=18 and 5, respectively). This library was constructed by Dr. Paul Keim and Dr. Virginia Coryell.

text: Glycine max

text: EcoRI

text: XhoI

text: Williams seedlings, minus the cotyledons

text: plasmid

text: pBluescript II XR


characteristic: INSDC first public

text: live

text: Generic

text: Williams

text: This cDNA library was constructed from mRNA isolated from cotyledons of 8-day-old 'Williams' seedlings which were propagated on paper towels with distilled water for 3 days (etiolated), then greenhouse grown for 5 days in potting soil. The cotyledons were flash-frozen in liquid nitrogen. Stratagene's cDNA Synthesis Kit (catalog number 200401) was used to synthesize the cDNA. First-strand synthesis was performed with 5-methyl dCTP, hence the ligated cDNA was hemimethylated. A modification of Stratagene's first-strand synthesis primer was used. An 'anchor' nucleotide (V=A, C, or G) was added to the 3' end of the primer [GAGAGAGAGAGAGAGAGAGAACTAGTCTCGAG(T)18V] to anchor the primer at the 5' end of the poly(A) tract. After second strand synthesis, the cDNA ends were filled in with cloned Pfu DNA, ligated to EcoRI adapters and subsequently phosphorylated. The cDNA was then precipitated and redissolved in sterile, RNase-, DNase-free water. The XhoI site within the first-strand synthesis primer was then restricted by digestion with XhoI from Promega (40U/ul); all XhoI sites in the cDNA would be protected by their hemimethylated status. The cDNA constructs were size-fractionated with a 500 bp cutoff, using GibcoBRL Life Technologies' cDNA Size Fractionation column. The column eluent was then precipitated, redissolved, and ligated into Stratagene's pBluescript II XR Predigested vector (pBluescript II SK(+) vector that has been digested with EcoRI and XhoI, and phosphorylated by Stratagene). 100% of the white and 87.5% of the blue colonies appear to contain recombinant plasmids with cDNA inserts, based on size (n=28 and 8 respectively). This library was constructed by Dr. Paul Keim and Dr. Virginia Coryell.

text: Glycine max

text: EcoRI

text: XhoI

text: Cotyledons of 8-day-old 'Williams' seedlings

characteristic: title

text: plasmid

text: pBluescript II XR


text: live

text: Generic

text: Delsoy 5710

text: This cDNA library was constructed from mRNA isolated from 'Desloy 5710' seedling roots. Tissue was taken from 7-day-old seedlings that had been propagated on paper towels with distilled water. Tissue was taken from the tip to the first lateral root, usually about 3cm from the tip, and flash-frozen in liquid nitrogen. Stratagene's cDNA Synthesis Kit (catalog number 200401) was used to synthesize the cDNA. First-strand synthesis was performed with 5-methyl dCTP, hence the ligated cDNA was hemimethylated. A modification of Stratagene's first-strand synthesis primer was used. An 'anchor' nucleotide (V=A, C, or G) was added to the 3' end of the primer [GAGAGAGAGAGAGAGAGAGAACTAGTCTCGAG(T)18V] to anchor the primer at the 5' end of the poly(A) tract. After second strand synthesis, the cDNA ends were filled in with cloned Pfu DNA, ligated to EcoRI adapters and subsequently phosphorylated. The cDNA was then precipitated and redissolved in sterile, RNase-, DNase-free water. The XhoI site within the first-strand synthesis primer was then restricted by digestion with XhoI from Promega (40U/ul); all XhoI sites in the cDNA would be protected by their hemimethylated status. The cDNA constructs were size-fractionated with a 500 bp cutoff, using GibcoBRL Life Technologies' cDNA Size Fractionation column. The column eluent was then precipitated, redissolved, and ligated into Stratagene's pBluescript II XR Predigested vector (pBluescript II SK(+) vector that has been digested with EcoRI and XhoI, and phosphorylated by Stratagene). 100% of the white and blue colonies appear to contain recombinant plasmids with cDNA inserts, based on size (n=24 and 6, respectively). This library was constructed by Dr. Paul Keim and Dr. Virginia Coryell.

characteristic: description

text: Glycine max

text: EcoRI

text: XhoI

text: 'Desloy 5710' seedling roots

text: plasmid

text: pBluescript II XR


characteristic: INSDC last update

text: live

text: Generic

text: cDNA made by oligo-dT priming. Directionally cloned into SalI/NotI sites using the following 5' adaptor: tcgacccacgcgtccg and 3' adaptor: gactagttctagatcgcgagcggccgcccttttttttttttttt. Average insert size 1.3kb. Primary library, non-amplified. Library constructed by N. Pollet, PhD. REF: Gawantka, V., Pollet, N., Delius, H., Vingron, M., Pfister, R., Nitsch, R., Blumenstock, C., and Niehrs, C. (1998) Gene expression screening in Xenopus identifies molecular pathways, predicts gene function and provides a global view of embryonic patterning. Mech. Dev. 77:95-141. Note: This is a Xenopus Gene Collection (XGC) library.

text: stage 13

text: XL1-Blue, F+

text: Xenopus laevis

text: NotI

text: SalI

text: whole embryo

text: Xenla 13

text: plasmid (ampicillin resistant)

text: pBluescript KS+ (Stratagene)


text: Peking Union Medical College; Chinese Academy of Medical Science

text: live

characteristic: INSDC status

text: Generic

text: Endothelial cells

text: Homo sapiens

text: Venae

text: Human umbilical vein Endothelial Cell cDNA library

text: Lambda Zap Express


text: Chinese Academy of Tropical Agriculture Science

text: live

text: Generic

characteristic: NCBI submission model

text: Hevea brasiliensis

text: Hevea brasiliensis RRIM600


text: live

text: Generic

characteristic: NCBI submission package

text: Endothelial cells

text: Homo sapiens

text: Venae

text: Human umbilical vein Endothial Cell cDNA library

text: Lambda Zap Express


text: Ludwig Institute for Cancer Research

text: live

text: Generic

text: A mini-library was made by cloning products derived from ORESTES PCR (U.S. Letters Patent application No. 196,716 - Ludwig Institute for Cancer Research) profiles into the pUC 18 vector. Reverse transcription of tissue mRNA and cDNA amplification were performed under low stringency conditions.

text: Adult

characteristic: organ

text: Homo sapiens

characteristic: organism

text: SmaI

text: SmaI

text: Plasmid


text: Ludwig Institute for Cancer Research

text: live

text: Generic

text: Adult

text: breast

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid

characteristic: vector


text: Ludwig Institute for Cancer Research

text: live

text: Generic

text: Adult

text: colon

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: Ludwig Institute for Cancer Research

text: live

text: Generic

text: Adult

text: leiomios

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: Ludwig Institute for Cancer Research

text: live

text: Generic

text: Adult

text: pnet

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: Ludwig Institute for Cancer Research

text: live

text: Generic

text: Adult

text: stomach

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: Harvard University, Howard Hughes Medical Institute

text: live

text: Generic

text: Library constructed using SuperScript Plasmid Library kit (Life Technologies). cDNA made by oligo-dT priming. Size-selected by column fractionation; average insert size 1.08 kb. Primary library, unamplified.

text: Adult

text: Pancreas

text: Homo sapiens

text: Not 1

text: Sal 1

text: Islets of Langerhans

text: Human Pancreatic Islets

text: plasmid (ampicillin resistant)


text: live

text: Generic

text: Mus musculus

text: Image Mouse Libraries