text: University of St Andrews

text: live

text: Generic

characteristic: NCBI submission package

text: Takifugu rubripes

text: fast skeletal muscle

characteristic: tissue-type

text: Forward subtracted cDNA library from fast skeletal muscle


status: PUBLIC

characteristic: External Id

text: University of St Andrews

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Takifugu rubripes

characteristic: organism

text: fast skeletal muscle

tag: attribute

text: Reverse subtracted library from fast skeletal muscle

characteristic: title


text: University of Missouri at Columbia

text: live

text: Generic

text: Adult

characteristic: dev-stage

text: Uterine

characteristic: organ

text: Ovis aries

text: female

characteristic: sex

text: Endometrium

text: Sheep endometrium cDNA library


text: ANRI

text: live

text: Generic

text: Normalized cow cDNA intestinal library in pCMVsport6.1, constructed from equimolar mRNA pools derived from 5 sources, 4 lactating intestinal, 1 neonatal intestinal 4/5 Lactating, Proximal Duodenum, Jejunum, Distal Ileum, Colon, 1/5 Neonatal, Proximal Duodenum, Jejunum, Distal Ileum

characteristic: description

text: Lactating, Neonatal

text: DH10B TonA

characteristic: lab-host

text: Intestine

text: Bos taurus

text: NotI

text: EcoRI

text: Female

text: Holstein

characteristic: strain

text: Epithelial, Muscle

text: BARC 8BOV

text: Plasmid

characteristic: vector


text: University of Freiburg

text: live

text: Generic

characteristic: NCBI submission package

text: rst 5prime RACE was performed using the FirstChoice RLM-RACE Kit (Ambion). RACE products were subcloned into pCRII-TOPO (Invitrogen).

text: embryo (0-24h)

text: Drosophila melanogaster

text: total RNA derived 5prime RACE of rst

text: pCRII-TOPO (Invitrogen)


text: National Cancer Institute / NIH

text: live

text: Generic

text: 1st strand cDNA was primed with an oligo(dT) primer [GCGGCTGAAGACGGCCTATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded cDNA was ligated to a DraIII adaptor [GGCCUACUGG], digested and directionally cloned into distinct DraIII sites of the pME18S-FL3. Library was size selected for 1.0 kb, with a average insert size of ~1.2kb. Library constructed by Yutaka Suzuki (University of Tokyo Institute of Medical Science). Custom primers recommended for sequencing: 5' end primer 5'-GGATGTTGCCTTTACTTCTA-3' and 3' end primer 5'-CGACCTGCAGCTCGAGCACA-3'. Note: This is a Zebrafish Gene Collection (ZGC) library

text: DH10B TonA

text: gut

text: Danio rerio

text: DraIII

text: DraIII

text: 13 pooled, includes stomach, intestine, liver and pancreas

characteristic: tissue-type

text: phagemid


characteristic: External Id

text: National Cancer Institute / NIH

text: live

text: Generic

text: DH10B TonA

text: testis

text: Danio rerio

text: DraIII

text: DraIII

text: pooled

text: phagemid


text: University of Cape Town

characteristic: INSDC center name

text: live

text: Generic

characteristic: cell-line

text: embryonic day 10.5 post-fertilization

text: Mus musculus

text: olfactory neuro-epithelium

text: E10.5 olfactory placode OP27 cells


text: The Canadian Potato Genome Project - BioAtlantech

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Shepody

characteristic: cultivar

text: supplier: Developmental series. Plants from pathogen-free Solanum tuberosum var. Shepody, clone 1756, nuclear stock were grown in a screenhouse under natural conditions. RNA was isolated from stolon tissue.

text: Solanum tuberosum

text: EcoRI

text: XhoI

text: Stolon

text: Stolon

text: pBluescript II SK(+) XR


text: CIAD

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: hemocytes

characteristic: cell type

text: adult

text: Penaeus vannamei

characteristic: organism

text: blood

text: Litopenaeus vannamei blood adult


text: National Cheng Kung University

text: live

text: Generic

text: Phalaenopsis equestris

text: EcoR I

text: Xho I

text: hermaphrodite

characteristic: sex

text: Flower bud

text: Flower bud of Phalaenopsis equestris

characteristic: title

text: Plasmid

text: pBluescript SK


text: National Cheng Kung University

text: live

text: Generic

text: Phalaenopsis violacea

text: EcoR I

text: Xho I

text: hermaphrodite

text: Flower bud

text: Flower bud of Phalaenopsis violacea

text: Plasmid

text: pBluescript SK


text: Hokkaido University Tokyo Office

text: live

text: Generic

text: fry

characteristic: dev-stage

text: Oryzias latipes

text: whole-fry

text: Oryzias latipes whole-fry


text: Hokkaido University Tokyo Office

text: live

text: Generic

text: adult

text: Oryzias latipes

text: liver

text: Oryzias latipes liver adult


text: National Institute of Agricultural Science and Technology

text: live

text: Generic

text: treated with tunicamycin

characteristic: description

text: E.coli XLI-Blue MRF', SOLR

characteristic: lab-host

text: Bombyx mori

text: EcoRI

text: XhoI

text: Bombyx mori Bm5 Cell cDNA Library

text: Uni-ZAP XR


text: National Institute of Genetics

text: live

text: Generic

characteristic: NCBI submission package

text: fry stage 40

text: Oryzias latipes

text: mixture of female and male

text: Hd-rR

characteristic: strain

text: whole embryo

text: MF01FFA cDNA


characteristic: External Id

text: National Institute of Genetics

text: live

text: Generic

text: organogenesis stage 35

text: Oryzias latipes

text: mixture of female and male

text: Hd-rR

text: whole embryo

characteristic: tissue-type

text: MF015DA cDNA


characteristic: INSDC center name

text: live

text: Generic

text: Shepody

text: supplier: Developmental series. Sterile stem sections from pathogen-free Solanum tuberosum var. Shepody, clone 1756, nuclear stock were cultured in Magenta boxes containing 1/10 strength MS medium, solidified with 0.8% (w/v) Phytagar. Roots of all sizes, originating from the stem bottoms were collected from these cultures, washed to remove residual Phytagar, and used in RNA isolations and library construction.

text: Solanum tuberosum

text: EcoRI

text: XhoI

text: Root

text: In vitro Root

text: pBluescript II SK(+) XR

characteristic: vector


text: Norwegian School of Veterinary Science

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Pre-smolt

text: XL 10-Gold

text: Salmo salar

text: EcoRI

text: XhoI

text: Brain

text: Atlantic salmon Brain cDNA library

text: Plasmid

text: pBluescript II SK (+) XR


text: Norwegian School of Veterinary Science

text: live

characteristic: INSDC status

text: Generic

text: Pre-smolt

text: XL 10-Gold

text: Salmo salar

text: EcoRI

text: XhoI

text: Eye

text: Atlantic salmon Eye cDNA library

text: Plasmid

text: pBluescript II SK (+) XR


text: Norwegian School of Veterinary Science

text: live

text: Generic

characteristic: NCBI submission model

text: Pre-smolt

text: XL 10-Gold

text: Salmo salar

text: EcoRI

text: XhoI

text: Gills

text: Atlantic salmon Gills cDNA library

text: Plasmid

text: pBluescript II SK (+) XR


text: Norwegian School of Veterinary Science

text: live

text: Generic

text: Pre-smolt

text: XL 10-Gold

text: Salmo salar

text: EcoRI

text: XhoI

text: Head kidney

text: Atlantic salmon Head kidney cDNA library

text: Plasmid

text: pBluescript II SK (+) XR


text: Norwegian School of Veterinary Science

text: live

text: Generic

text: Pre-smolt

text: XL 10-Gold

text: Salmo salar

text: EcoRI

text: XhoI

text: Heart

text: Atlantic salmon Heart cDNA library

text: Plasmid

text: pBluescript II SK (+) XR


text: Norwegian School of Veterinary Science

text: live

text: Generic

text: Pre-smolt

text: XL 10-Gold

text: Salmo salar

text: EcoRI

text: XhoI

text: Intestine

text: Atlantic salmon Intestine cDNA library

text: Plasmid

text: pBluescript II SK (+) XR


text: Norwegian School of Veterinary Science

text: live

text: Generic

text: Pre-smolt

text: XL 10-Gold

text: Salmo salar

text: EcoRI

text: XhoI

text: Kidney

text: Atlantic salmon Kidney cDNA library

text: Plasmid

text: pBluescript II SK (+) XR


text: Norwegian School of Veterinary Science

text: live

text: Generic

text: Pre-smolt

text: XL 10-Gold

text: Salmo salar

text: EcoRI

text: XhoI

text: Liver

text: Atlantic salmon Liver cDNA library

text: Plasmid

text: pBluescript II SK (+) XR


text: Norwegian School of Veterinary Science

text: live

text: Generic

text: Pre-smolt

text: XL 10-Gold

text: Salmo salar

text: EcoRI

text: XhoI

text: Ovaries

text: Atlantic salmon Ovaries cDNA library

text: Plasmid

text: pBluescript II SK (+) XR


text: Norwegian School of Veterinary Science

text: live

text: Generic

text: Pre-smolt

text: XL 10-Gold

text: Salmo salar

text: EcoRI

text: XhoI

text: Skin

text: Atlantic salmon Skin cDNA library

text: Plasmid

text: pBluescript II SK (+) XR


text: Norwegian School of Veterinary Science

text: live

text: Generic

text: Pre-smolt

text: XL 10-Gold

text: Salmo salar

text: EcoRI

text: XhoI

text: Spleen

text: Atlantic salmon Spleen cDNA library

text: Plasmid

text: pBluescript II SK (+) XR


text: Norwegian School of Veterinary Science

text: live

text: Generic

text: Pre-smolt

text: XL 10-Gold

text: Salmo salar

text: EcoRI

text: XhoI

text: Swimbladder

text: Atlantic salmon Swimbladder cDNA library

text: Plasmid

text: pBluescript II SK (+) XR


text: Norwegian School of Veterinary Science

text: live

text: Generic

text: Pre-smolt

text: XL 10-Gold

text: Salmo salar

text: EcoRI

text: XhoI

text: Testis

text: Atlantic salmon Testis cDNA library

text: Plasmid

text: pBluescript II SK (+) XR


text: Norwegian School of Veterinary Science

text: live

text: Generic

text: Pre-smolt

text: XL 10-Gold

text: Salmo salar

text: EcoRI

text: XhoI

text: White muscle

text: Atlantic salmon White muscle cDNA library

text: Plasmid

text: pBluescript II SK (+) XR


text: National Taiwan University

text: live

text: Generic

text: 2-year-old Taiwania seedlings were obtained from the Experimental Forest of National Taiwan University. The cDNA library was constructed by pGEM-T Easy vector system (Promega).

text: Taiwania cryptomerioides

text: entire seedling

text: Taiwania seedling cDNA library

text: Plasmid

text: pGEM-T Easy


text: IRD-CNRS-Universite de Perpignan-CIAT

text: live

text: Generic

characteristic: cultivar

text: cDNA was synthesized from RNA obtained from inoculated cassava plants challenged with Xanthomonas axonopodis pv manihotis strain Cio-151. Tissues were collected at 12, 24, 48, 72 hpi and 7, 15,30 days after inoculation.

text: Stem

characteristic: organ

text: Manihot esculenta

text: EcoRI

text: EcoRI

text: MBra685 cassava stem pGMTEasy

text: plasmid

text: pGMTEasy


text: Prince of Songkhla University

text: live

text: Generic

text: Total RNA was isolated from haemocytes of Penaeus monodon. The mRNA was further purified, reverse transcribed and cloned to Zap Express Vector. The cDNA library was generated as part of Penaeus monodon EST project

text: Haemocytes

text: Penaeus monodon

characteristic: organism

text: EcoR I

text: Xho I

text: Haemocyte-WSSV infected cDNA library

text: Phage

text: Zap Express Vector


text: Institute of Biochemistry and Biophysics, Polish Academy of Sciences

text: live

text: Generic

text: vegetative

text: Nicotiana tabacum

text: developing leaves

text: Nicotiana tabacum LABurley21 developing leaves

characteristic: title


text: State Research Institute Geisenheim

text: live

text: Generic

text: Boerner

text: cDNA subtraction with auxin treated and none treated root tip tissue. The cultivar 'Boerner' is a cross breeding: Vitis riparia 183 Geisenheim x Vitis cinerea Arnold

text: adult plants

characteristic: dev-stage

text: root

text: Vitis riparia x Vitis cinerea

text: EcoRI

text: SpeI

text: male

characteristic: sex

text: root tip tissue

text: Boerner cDNA subtraction library

text: Plasmid

text: pGEM-Teasy Vector


text: University of Cape Town

text: live

text: Generic

text: 5' end may contain EcoRI site and linker i.e. GAATTCGGCACGAAG

characteristic: description

text: Xerophyta humilis

text: EcoRI

text: XhoI

text: Xerophyta humilis Uni-ZAP XR cDNA library

text: Phagemid

text: pBluescript SK


text: Guangxi University, China Agricultural University, Zhejiang University

text: live

text: Generic

characteristic: NCBI submission package

text: Mycellium, conidium, germinating conidium, swelling appressorium, mature appressorium, penetration peg

text: Pyricularia grisea

text: Normalized Magnaporthe grisea cDNA pGEM-T Easy library

text: pGEM-T Easy


text: Dipartimento di Biologia Vegetale, Universita degli Studi di Torino

text: live

text: Generic

text: vegetative stage

text: Oidiodendron maius

text: Zn strain

characteristic: strain

text: mycelium

text: O. maius grown on liquid medium 0.05% ZnChloride added

text: Phagemid

text: UniZapXR (Stratagene,USA)


characteristic: External Id

text: Washington University School of Medicine

text: live

text: Generic

text: The library was constructed by Irina Ronko and Dr. Makedonka Mitreva (mmitreva@watson.wustl.edu) at Washington University, St. Louis. Oligo(dT)-SL1 PCR based library. Pristionchus pacificus dauer stage cDNA PCR products of size >400 nucleotides containing SL1 on the 5' end and oligo(dT) on the 3' end were non-directionally cloned into pCRII-TOPO(Invitrogen) following the Topo TA cloning protocol. P. pacificus strain PS312 was provided by the Caenorhabditis Genetic Center, dauer and RNA extraction by B. Chiappeli at Divergence,Inc., St. Louis. MO.

text: dauer stage

characteristic: lab-host

text: Pristionchus pacificus

text: 5': EcoRI

text: 3': EcoRI

text: PS 312

text: Pristionchus pacificus dauer stage SL1 TOPO v1

text: plasmid (kanamycin and ampicillin resistant)

text: pCRII-TOPO (Invitrogen)


text: Zhejiang University, China Agricultural University, Guangxi University

characteristic: INSDC center name

text: live

text: Generic

text: Pyricularia grisea

characteristic: tissue-type

text: Normalized Magnaporthe grisea cDNA pRS423 library


text: UC Davis, Department of Pomology

characteristic: INSDC first public

text: live

text: Generic

text: Juice Sac

characteristic: cell type

text: Washington Navel Orange

text: Developing citrus fruits were harvested from trees growing in the 'Citrus variety collection' in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected on June 17, 2003, between 8 to 9 am and stored at 4C. The juice sac tissue was dissected out and used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The primary library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes.

text: 38 DAFB fruit sample-collected June 17, 2003

text: Fruit

text: Citrus sinensis

text: SfiIA

text: SfiIB

text: Hermaphrodite

text: Developing fruit juice sac at 38 DAFB

text: Plasmid

characteristic: vector


text: UC Davis, Department of Pomology

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Peel/Rind

text: Washington Navel Orange

text: Developing citrus fruits were harvested from trees growing in the 'Citrus variety collection' in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected on June 17, 2003, between 8 to 9 am and stored at 4C. The entire peel (including both flavedo and albedo) was used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The primary library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes.

text: Young fruit sample-collected June 17, 2003

text: Fruit

text: Citrus sinensis

text: SfiIA

text: SfiIB

text: Hermaphrodite

text: Developing fruit peel at 38 DAFB

text: Plasmid


text: UC Davis, Department of Pomology

text: live

text: Generic

text: Washington Navel Orange

characteristic: cultivar

text: Developing citrus fruits were harvested from trees growing in the 'Citrus variety collection' in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected on June 3, 2003, between 8 to 9 am and stored at 4C. The entire fruit was used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The primary library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes.

text: Young fruit sample-collected June 3 2003

text: Fruit

characteristic: organ

text: Citrus sinensis

characteristic: organism

text: SfiIA

text: SfiIB

text: Hermaphrodite

text: Developing fruit 24 DAFB

text: Plasmid


text: UC Davis, Department of Pomology

text: live

text: Generic

text: Falvedo

text: Washington Navel Orange

text: Developing citrus fruits were harvested from trees growing in the 'Citrus variety collection' in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected on July 29, 2003, between 8 to 9 am and stored at 4C. The flavedo tissue was dissected out of developing fruit (80 DAFB) and used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The primary library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes.

text: Developing fruit sample-collected July 29, 2003

characteristic: dev-stage

text: Fruit

text: Citrus sinensis

text: SfiIA

text: SfiIB

text: Hermaphrodite

characteristic: sex

text: Developing fruit flavedo at 80 DAFB

characteristic: title

text: Plasmid


text: UC Davis, Department of Pomology

text: live

text: Generic

characteristic: NCBI submission package

text: Albedo

text: Washington Navel Orange

text: Developing citrus fruits were harvested from trees growing in the 'Citrus variety collection' in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected on July 29, 2003, between 8 to 9 am and stored at 4C. The albedo tissue was dissected out of developing fruit (80 DAFB) and used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The primary library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes.

characteristic: description

text: Developing fruit albedo-collected July 29, 2003

text: Fruit

text: Citrus sinensis

text: SfiIA

text: SfiIB

text: Developing fruit albedo at 80 DAFB in pTriplEx2 vector

text: Plasmid


characteristic: External Id

text: UC Davis, Department of Pomology

text: live

text: Generic

text: Albedo

text: Washington Navel Orange

text: Developing citrus fruits were harvested from trees growing in the 'Citrus variety collection' in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected on October 22, 2003, between 8 to 9 am and stored at 4C. The albedo tissue was dissected out of developing fruit (165 DAFB) and used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The primary library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes.

text: Developing fruit albedo-collected October 22, 2003

characteristic: lab-host

text: Fruit

text: Citrus sinensis

text: SfiIA

text: SfiIB

text: Hermaphrodite

text: Developing fruit albedo at 165 DAFB

text: Plasmid


text: UC Davis, Department of Pomology

characteristic: INSDC center name

text: live

text: Generic

text: Falvedo

text: Washington Navel Orange

text: Developing citrus fruits were harvested from trees growing in the 'Citrus variety collection' in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected on October 22, 2003, between 8 to 9 am and stored at 4C. The flavedo tissue was dissected out of developing fruit (165 DAFB) and used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The primary library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes.

text: Developing fruit sample-collected October 22, 2003

text: Fruit

text: Citrus sinensis

text: SfiIA

text: SfiIB

text: Hermaphrodite

text: Developing fruit flavedo at 165 DAFB

text: Plasmid


text: Animal and Natural Resources Institute

characteristic: INSDC first public

text: live

text: Generic

text: Equimolar amounts of mRNA extracted from fundic and pyloric abomasums of 18 and 21 week old steers. Exposure to Ostertagia ostertagi was initiated at 15 weeks of age. fundic and pyloric abomasum

text: Multiple

text: DH10B T1 phage resistant

text: Abomasum

text: Bos taurus

text: EcoRV

text: NotI

text: Male

text: Holstein

characteristic: strain

text: Pooled

characteristic: tissue-type

text: BARC 9BOV

text: plasmid


text: Virginia Polytechnic Institute and State University

characteristic: INSDC last update

text: live

text: Generic

text: 10-11 day-old chick

text: Gallus gallus

text: White Leghorn

text: pineal gland

text: Chicken pineal cDNA library


text: Washington University School of Medicine

text: live

characteristic: INSDC status

text: Generic

text: Cloned unidirectionally. Poly (A)+ RNA was concentrated and purified using Dynabeads (Dynal) and mRNA eluted for 1ST strand synthesis. 1st strand cDNA created using MMLV RT(PowerScript, CLontech) and primedwith oligo(dT) with XhoI site (primer CDSIII/3-XhoI ) and 5' SMART 'anchor' added using chimericDNA-RNA oligo (SMART-NotI-r-GGG).SMART-NotI-r-GGG was used in case a PCR using SMART template was also desired. 15 PCR cycles were done using 1st strand and primers specific to SL1 leader sequence (SL1-NotI) and 3' end (XhoI-No-dT). dscDNA was digested using XhoI/NotI, fractionated on Chroma-spin 400 columns(Clontech) and ligated to digested pGEM -11zf(+) plasmid. Chemically competent cells were used as host.

text: Meloidogyne incognita

text: XhoI

text: NotI

text: juvenile

text: Meloidogyne incognita J3 J4 SL1 pGEM

text: plasmid (AMP resistant)

text: pGEM -11zf(+)(Promega)

characteristic: vector


text: live

text: Generic

characteristic: NCBI submission model

text: Cloned unidirectionally. Poly(A)+ RNA was concentrated and purified using Dynabeads (Dynal) and mRNA eluted for first strand synthesis. First strand cDNA was created using MMLV RT (Powerscript, Clontech) and primed with oligo(dT) with XhoI site (primer CDSIII/3-XhoI) and 5'SMART 'anchor' added using chimeric DNA-RNA oligo (SMART-NotI-r-GGG). 12 PCR cycles were done using first strand and primers specific to SMART oligo (5' PCR-primer) and 3' end(XhoI-No-dT). Double stranded /gsc/scripts/gsc/estcDNA was digested using XhoI/NotI, fractioned on Chroma-spin 400 columns (Clontech) and ligated to digested pGEM-11zf(+) plasmid. Chemically competent cells were used as host cells. Library materials provided by Dr. David Bird of North Carolina State University. Library construction by Jeff Rousch and Joanna Carlson of North Carolina State University. See www.nematode.net for additional project information.

text: adult

text: Meloidogyne incognita

text: XhoI

text: NotI

text: female

text: whole organism

text: Meloidogyne incognita female SMART pGEM

text: plasmid (ampicillin resistant)

text: pGEM-11zf(+) (Promega)


text: China Agricultural University

text: live

text: Generic

text: Inbred line Han21

characteristic: cultivar

text: Zea mays

text: water stress specific subtracted cDNA library of maize seedlings


text: Animal and Natural Resources Institute

text: live

text: Generic

text: Normalized library of pooled RNA isolated from whole brain, ultimobranchial gland, parathyroid gland, cecal tonsil and primordial germ cells Multiple

text: Gallus gallus

characteristic: organism

text: NotI

text: EcoRI

text: Leghorn and broiler

text: BARC_3GAL chicken mixed tissue

text: plasmid

text: pBluescript, SK+, Stratagene


text: The University of Alabama at Birmingham

text: live

text: Generic

text: lymphocyte

characteristic: cell type

text: Lymphocyte mRNA, encoding leucine-rich repeat proteins, somatically diversified versions of the single germline gVLR. Unstimulated larvae. Directionally cloned.

text: Petromyzon marinus

text: Sea lamprey unstimulated larvae

text: Lambda ZAP Express (Stratagene)


text: The University of Alabama at Birmingham

text: live

text: Generic

text: lymphocyte

text: Lymphocyte mRNA, encoding leucine-rich repeat proteins, somatically diversified versions of the single germline gVLR. Immune stimulated larvae. Randomly cloned. PCR subtracted.

text: Petromyzon marinus

text: Sea lamprey immune stimulated larvae

text: pGEM-T Easy (Promega)


text: CNRS/EPHE/UPVD

text: live

text: Generic

text: Circulating hemocytes

text: The library was designed with 500 ng of total RNA using the creator SMART cDNA library construction kit (Clontech).

text: Bg.Bra strain

characteristic: isolate

text: Biomphalaria glabrata

text: hermaphrodite

characteristic: sex

text: BgHC

text: Plasmid

text: pDNR-LIB


text: live

text: Generic

characteristic: NCBI submission package

text: Total RNA was used for cDNA library construction. Reverse transcription and cDNA normalisation used standard procedures. To facilitate cDNA amplification and subsequent cloning, a 'lone' linker, which is an annealing product of two primers AscI A (5'-GAGATATTAGGCGCGCCTACTC) and AscI B (5'-GAGTAGGCGCGCCTAATAT), was added to both ends of all random cDNAs. Three rounds of normalization were performed consecutively. The normalized cDNAs were digested with AscI and NotI, and cDNA fragments of 400 bp to 1 kb were purified, ligated into the PVX vector construct pGR106.

text: Nicotiana benthamiana

text: AscI

text: NotI

text: Leaf

text: Subset of normalised cDNA library cloned in PVX

characteristic: title

text: Virus

text: Potato Virus X


characteristic: External Id

text: University of Pittsburgh

text: live

text: Generic

text: Brassica juncea

text: Brassica juncea leaf disc explants


text: Mahidol University

text: live

text: Generic

text: Total RNA was isolated from eyestalk neural tissues of Penaeus monodon. The mRNA was further purified, reverse transcribed and cloned to Uni-ZAP XR Vector. The cDNA library was generated as part of Penaeus monodon EST project

characteristic: description

text: Eyestalks

characteristic: organ

text: Penaeus monodon

text: EcoR I

text: Xho I

text: Female

text: Eyestalk cDNA library

text: Phage

text: Uni-ZAP XR Vector


text: Wuhan University

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: Nilaparvata lugens

text: biotype GU

text: Nilaparvata lugens biotype GU


text: Martin-Luther-Universitat Halle

text: live

text: Generic

text: Steffi

text: 10 day old seedling

characteristic: dev-stage

text: Hordeum vulgare subsp. vulgare

text: leaf

characteristic: tissue-type

text: Hordeum vulgare subsp. vulgare leaf 10 day old seedling


text: Martin-Luther-Universitat Halle

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Steffi

text: 9 day old seedling

text: Hordeum vulgare subsp. vulgare

text: leaf

text: Hordeum vulgare subsp. vulgare leaf 9 day old seedling


text: Biochimie et Physiologie Moleculaire des Plantes BPMP UMR 5004 AgroM-CNRS-INRA-UM2

text: live

text: Generic

characteristic: NCBI submission model

text: homocaryon h1,homocaryon h7,dicaryon d2

text: Sample name: ESTHcyl ; Plant growth place: obscurity at 26 oC ; Soil conditions: liquid ; Sowing date: 01/06/1999 ; Harvesting date: 15/06/1999 ; Stress date: 13/06/1999 ; Description: The mycelia from 3 strains D2, h1, h7 were grown on 3 media (standard, potassium-free and phosphate-free)

text: hyphal

characteristic: lab-host

text: Hebeloma cylindrosporum

text: mycelium

text: ESTHcyl

characteristic: vector


text: Hopkins Marine Station, Stanford University

text: live

text: Generic

text: egg

text: Strongylocentrotus purpuratus

characteristic: organism

text: Egg cDNA macroarray SUGP

text: psport


text: National Key Laboratory of Crop Genetic Improvement

text: live

text: Generic

text: Gossypium barbadense

text: root

text: Gossypium barbadense root seedlings with first full leaves expanding


text: Centro de Investigaciones Biologicas del Noroeste, S.C

text: live

text: Generic

text: Penaeus vannamei

text: CIB

characteristic: strain

text: Hepatopancreas PGEM (promega)


text: University of Nevada

text: live

text: Generic

text: Cell Suspension Culture

characteristic: cell type

text: Cabernet Sauvignon

characteristic: cultivar

text: A cDNA library from a Cabernet Sauvignon cell suspension culture.

text: Vitis vinifera

text: Hermaphrodite

characteristic: sex

text: CabSau Cell Culture (CELu0001)

text: Plasmid


characteristic: External Id

text: NONG WOO BIO CO.,LTD.

text: live

text: Generic

characteristic: NCBI submission package

text: Phytophthora-induced cDNA library of pepper roots sampled on 7th day after infection was synthesized.

text: 7-8 weeks-old Roots

text: Capsicum annuum

text: EcoRI

text: hermaphrodite

text: root

characteristic: title

text: plasmid


text: National Cancer Institute / NIH

characteristic: INSDC center name

text: live

text: Generic

text: 1st strand cDNA was primed with an oligo(dT) primer [GCGGCTGAAGACGGCCTATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded cDNA was ligated to a DraIII adaptor [GGCCUACUGG], digested and directionally cloned into distinct DraIII sites of the pME18S-FL3. Library was size selected for 1.0 kb, with a average insert size of ~1.2kb. Library constructed by Yutaka Suzuki (University of Tokyo Institute of Medical Science). Custom primers recommended for sequencing: 5' end primer 5'-GGATGTTGCCTTTACTTCTA-3' and 3' end primer 5'-CGACCTGCAGCTCGAGCACA-3'. Note: This is a Zebrafish Gene Collection (ZGC) library

characteristic: description

text: DH10B TonA

text: embryo

characteristic: organ

text: Danio rerio

text: DraIII

text: DraIII

text: whole embryos staged from 2-8 hr postfertilization, approximately 2500 embryos total

text: phagemid


text: National Cancer Institute / NIH

characteristic: INSDC first public

text: live

text: Generic

text: DH10B TonA

text: heart

text: Danio rerio

text: DraIII

text: DraIII

text: Dissected hearts form 100 Zebrafish samples were pooled

characteristic: tissue-type

text: phagemid


text: National Cancer Institute / NIH

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: DH10B TonA

text: ovary

text: Danio rerio

text: DraIII

text: DraIII

text: contains eggs from one female, from all stages of development as well as support cells

text: phagemid


text: University of Missouri-Columbia

text: live

text: Generic

characteristic: NCBI submission model

text: Funding: A grant from the Monsanto Company to the University of Missouri. Genetic Source: Slaughterhouse-derived oocytes were collected, and after cumulous cell removal were used for germinal vesicle stage oocytes, or were matured in vitro (with cumulus cells attached), in vitro fertilized and cultured. In vivo produced 4-cell and blastocyst stage embryos were collected on days 3 and 6, respectively. Zonae Pellucidae were removed from the embryos prior to mRNA isolation. Expanded descriptions of how the tissues were collected can be found at the following URL: http://genome.rnet.missouri.edu/Swine/Methods.html. Library Construction (PCR Protocol): The amount of mRNA that was recovered from oocytes and embryos was quite limiting and was not sufficient for library production with a standard protocol. Therefore, PCR-based protocol was utilized for producing libraries. Poly-A RNA was isolated by using the MicroPoly(A) Pure kit from Ambion (cat. #1918). The mRNA was reverse transcribed with a NotI-tag-dT18 oligonucleotide and a SMART oligonucleotide (Clontech) modified to contain a SalI site to generate full-length cDNA with a sequence complementary to the SMART oligonucleotide. Sequences within the SMART and dT oligonucleotides were used as primers to amplify the cDNAs by PCR with pfu turbo polymerase (Stratagene). The resulting PCR products were purified, digested with NotI and SalI and size fractionated by using a Chroma Spin-400 followed by a Chroma Spin-1000 column (Clontech). Purified cDNA from each PCR reaction was ligated into the pCMV-SPORT6 vector. Preliminary Library Characterization: Randomly chosen clones from each library were analyzed by restriction digestion to determine average insert size (96 clones) and by sequencing (~3 96-well plates) to confirm library quality [e.g. the presence of short polyA+ tails, genomic DNA contamination (must be <1%), ribosomal RNA clones (must be <1%), etc.] and to provide a sequence database representing the predominant clones in each library. The clones were sequenced at the University of Missouri-Columbia DNA Core Facility. Bioinformatics work was performed by GK Springer's bioinformatics group (WG Spollen, JE Ries, A Guillen) in Computer Science and Health Management and Informatics Departments at the University of Missouri-Columbia. Clone Requests: Requests for clones should be made to the Director of the University of Missouri DNA Core facility at: porcine@rnet.missouri.edu.

text: Sus scrofa


text: University of Missouri-Columbia

text: live

text: Generic

text: Sus scrofa

text: pblivp


text: University of Missouri-Columbia

text: live

text: Generic

text: Sus scrofa

text: pblivv


text: University of Missouri-Columbia

text: live

text: Generic

text: Sus scrofa

text: pgvo


text: University of Kentucky

text: live

text: Generic

text: Ambystoma mexicanum

text: Limb Blastema and Proximal Limb Tissue collected from larvae on days 1-6 of regeneration

text: Math


text: University of Kentucky

text: live

text: Generic

text: Ambystoma tigrinum

text: Liver, Lung, Kidney, Heart, gonad, brain and gill tissues collected from metamorphosing larvae

text: AG


text: University of Kentucky

text: live

text: Generic

text: Ambystoma mexicanum

text: Tail Blastema adn Proximal Tail Tissue


text: University of Kentucky

text: live

text: Generic

text: Ambystoma tigrinum

text: Whole Brain tissue


text: University of Kentucky

text: live

text: Generic

text: Ambystoma tigrinum

text: Liver Tissue collected from viral infected larvae


text: University of Kentucky

text: live

text: Generic

text: Ambystoma tigrinum

text: Spleen tissue collected from viral infected larvae


text: University of Kentucky

text: live

text: Generic

text: Ambystoma tigrinum

text: Heart Tissue


text: University of Kentucky

text: live

text: Generic

text: Ambystoma mexicanum

text: Gill Tissue collected from larvae during T4 induced metamorphosis


text: University of Kentucky

text: live

text: Generic

text: Ambystoma mexicanum

text: Whole stage 22 Embryos


text: Tianjin Institute of Urologic Surgery

text: live

text: Generic

text: Poly A+ RNA were isolated from the tissues of renal cell carcinoma and normal kidney tissues respectively. Then double-strand cDNA were synthesized and restricted by Rsa I. The carcinoma cDNA were divided into two groups and ligated with either adaptor 1 or adaptor 2. After hybridizing with normal cDNA twice and undergoing nested PCR, the PCR products were cloned into PT-Adv vector and transformed E.Coli TOP10F'.

text: kidney

text: Homo sapiens

text: male

text: renal clear cell carcinoma

text: subtractive library of renal cell carcinoma

text: Plasmid

text: PT-Adv

characteristic: vector


text: University Stellenbosch

text: live

text: Generic

text: Merlot

text: 12 weeks post flowering

characteristic: dev-stage

text: Vitis vinifera

characteristic: organism

text: berry

text: mRNA from grapevine berries collected 12 weeks post flowering


text: Stanford University

text: live

text: Generic

text: This library represents the unique genes found in the second round of EST sequencing at Stanford University for the maize genome project. Sequences are present from library 949. Contigs were assembled using ZmDBAssembler and 2 representatives from each contig were selected for the Unigene set. All singlets were also selected. Library 947, also part of Unigene II, was submitted under NAME '1020 - Unigene II from Maize Genome Project'.

text: Zea mays

text: 1021 - Unigene II from Maize Genome Project


text: University H.Poincare - Nancy 1

text: live

text: Generic

text: cDNA library was prepared from total RNA as starting material using the Smart PCR cDNA library construction kit.

text: Paxillus involutus

characteristic: strain

text: Paxillus involutus cDNA, cadmium-treated mycelium, pGEM-T

text: Plasmid

text: pGEM-T.


text: University H. Poincare - Nancy 1

text: live

text: Generic

text: Paxillus involutus

text: Paxillus involutus cDNA

text: Plasmid

text: pGEM-T.


text: Northeast Forestry University

text: live

text: Generic

text: Tamarix hispida

text: Root

text: Tamarix hispida Willd. suppression subtractive hybridization cDNA library


text: Washington University School of Medicine

text: live

text: Generic

text: The library was constructed by Keliang Tang,Robert Cole and L.David Sibley at Washington University. cDNAs were synthesized from poly(A)+ RNAby oligo d(T) priming, size-selected and directionally cloned into the Uni-ZAP XR lambda vector (Stratagene). The primary library was mass excised as phagemids and rescued in SOLR cells. The plasmid library was recovered from the SOLR cells and transformed in mass into GC10 cells forsequencing. WARNING: the library may contain a small percentage contaminants from human fibroblast cells.

text: Tachyzoite

characteristic: lab-host

text: Toxoplasma gondii

text: EcoRI

text: XhoI

text: VEG

text: TgVEG118 Tachyzoite cDNA Library-2

text: pBluescript SK


text: The University of Georgia, Department of Plant Biology

text: live

text: Generic

characteristic: cultivar

text: The library was prepared from polyA+ RNA from 8-day-old roots harvested from BTx623 sorghum seedlings grown in hydroponic culture. HCl was added to a pH of 3.0 to some seedlings, KOH to a pH of 9.0 for others. Roots were harvested 3, 12 and 27 hr after addition and pooled for RNA preparation. Double-stranded cDNA was cloned unidirectionally into different DraIII sites of the pME18S-FL3 vector (5-prime DraIII site is CACTGTGTG, 3-prime DraIII site is CACCATGTG). XhoI excises the cDNA insert.

text: DH10B-T1 phage-resistant E. coli

text: Root

text: Sorghum bicolor

text: XhoI

text: XhoI

text: Acid- and alkaline-treated roots

text: Plasmid


characteristic: External Id

text: live

text: Generic

text: The library was prepared from polyA+ RNA harvested from 8-day-old hydroponically grown, BTx623 sorghum seedlings. For some plants, one-half of the second leaf ws crushed without damaging the midvein. For others, methyl jasmonate was added to the growth medium to a final concentration of 100 uM. Leaves were harvested 3 and 27 hr after treatment and pooled. Double-stranded cDNA was cloned unidirectionally into different DraIII sites of the pME18S-FL3 vector (5-prime DraIII site is CACTGTGTG, 3-prime DraIII site is CACCATGTG). XhoI excises the cDNA insert.

text: DH10B-T1 phage-resistant E. coli

text: Leaf

text: Sorghum bicolor

text: XhoI

text: XhoI

text: Wounded leaves

text: Plasmid


text: West China Hospital, Sichuan University

text: live

text: Generic

characteristic: NCBI submission package

text: Testis cells

characteristic: cell type

text: Rattus norvegicus

text: EcoRI

text: XhoI

text: Rattus norvegicus testis Lambda ZAP II cDNA Express Library

text: phagemid

text: Lambda ZAP II


text: Agriculture and Agri-food Canada

characteristic: INSDC center name

text: live

text: Generic

text: Marion

text: cDNA library made with lambda unizap-XR phagemid cloning kit (stratagene)

text: immature inflorescences

text: Avena sativa

text: EcoRI

text: XhoI

text: inflorescence

text: ACO cDNA from oat

characteristic: title

text: Plasmid

text: Bluescript SK+


text: Agriculture and Agri-food Canada

text: live

text: Generic

text: Lang

text: cDNA library of oat roots made with lambda unizap-XR phagemid cloning kit (stratagene). Library was produced at Iowa State University; Rayapati (1994) Theor Appl Gent 89:83-837.

characteristic: description

text: germinated 8 days

text: Avena sativa

text: EcoRI

text: XhoI

text: root

text: ISU cDNA from oat

text: Plasmid

text: Bluescript SK-


characteristic: INSDC first public

text: live

text: Generic

text: 1st strand cDNA was primed with a NotI-oligo(dT) primer. SalI adaptors were ligated following 2nd strand synthesis. Library not normalized.

text: E. coli DH5-alpha

text: Drosophila simulans

text: NotI

text: SalI

text: Drosophila simulans testis pSport1 library

text: plasmid, AmpR

text: pSPORT (BRL)


text: live

text: Generic

text: intestinal epithelium

text: Mus musculus

text: intestine

characteristic: tissue-type

text: Mouse intestinal epithelium


text: E. I. DuPont de Nemours and Company

characteristic: INSDC last update

text: live

text: Generic

text: Corn (Zea mays, B73) fertilized ear 3-12 DAP (full length)

text: Zea mays

text: NotI

text: SalI

text: seedling


text: E. I. DuPont de Nemours and Company

text: live

characteristic: INSDC status

text: Generic

text: Maize, stalk meristem. Identify genes that are expressed in the meristematic region within an internode.

text: Zea mays

text: NotI

text: SalI

text: stalk meristem


text: E. I. DuPont de Nemours and Company

text: live

text: Generic

characteristic: NCBI submission model

text: Corn (Zea mays L.) cob of 67 day old plants grown in green house (normalized)

text: Zea mays

text: EcoRI

text: XhoI

text: cob

text: pBluescript SK+


text: Department of Botany & Plant Sciences, University of California

text: live

text: Generic

text: Washington Navel

text: Fruits were collected January-March 2003 (Federici, Roose lab; Focht, Sievert & Robinson, Arpaia lab). Four samples related to storage conditions were produced: 1) fresh-picked in Mentone (Arnott Brothers Enterprises, Mentone, CA), 2) after 21 days storage at 5C at Kearney then transported to UC Riverside on ice, 3) after 5 additional days storage at 11C at Kearney, sampled immediately, 4) fruit grown in southern CA were obtained from Redlands Foothill Packing House after commercial packing, X-ray irradiated at 300 Gy by Surebeam, then stored 1 day at ambient temperature. Pulp tissue (juice vesicles) were collected. Tissues were snap frozen and then stored at -80C until further processing. Fenton (Close lab) purified RNA by the phenol method described in J. Japanese Soc. Hort. Sci. 1996. 64 (4): 809-814, purified poly(A) mRNA using a PolyATtrack mRNA Isolation System IV (Promega), produced a primary cDNA library using a lambda ZAP XR cDNA Synthesis Kit (Stratagene), then mass-excised one million pfu from the primary library to produce a phagemid population. Phagemids were plated, plasmid DNA purified, cDNA clones archived, and DNA sequences determined bi-directionally using an ABI3730 at the Arizona Genomics Institute, University of Arizona (Kim, Kudrna, Stum, Yost, Wing). Chromatogram files were downloaded by FTP to UC Riverside (by Close), then processed at UC Riverside (by Wanamaker, Close lab) using the HarvEST pipeline (http://harvest.ucr.edu) to remove vector and cloning oligo sequences and various contaminants, and to trim to a high quality region. Sequences that retained a phred 17 region of at least 100 bases were deposited to GenBank.

text: Commercially producing trees

text: E. coli TJC121

text: Citrus sinensis

text: EcoRI

text: XhoI

text: Pulp

text: Washington Navel Orange Stored Fruit Pulp cDNA Library

text: Phagemid pBluescript SK(-)

text: Lambda Uni-ZAP XR, excised phagemid

characteristic: vector


text: live

text: Generic

text: Washington Navel

text: Fruits were collected January-March 2003 (Federici, Roose lab; Focht, Sievert & Robinson, Arpaia lab). Four samples related to storage conditions were produced: 1) fresh-picked in Mentone (Arnott Brothers Enterprises, Mentone, CA), 2) after 21 days storage at 5C at Kearney then transported to UC Riverside on ice, 3) after 5 additional days storage at 11C at Kearney, sampled immediately, 4) fruit grown in southern CA were obtained from Redlands Foothill Packing House after commercial packing, X-ray irradiated at 300 Gy by Surebeam, then stored 1 day at ambient temperature. Rind tissue (juice vesicles) were collected. Tissues were snap frozen and then stored at -80C until further processing. Fenton (Close lab) purified RNA by the phenol method described in J. Japanese Soc. Hort. Sci. 1996. 64 (4): 809-814, purified poly(A) mRNA using a PolyATtrack mRNA Isolation System IV (Promega), produced a primary cDNA library using a lambda ZAP XR cDNA Synthesis Kit (Stratagene), then mass-excised one million pfu from the primary library to produce a phagemid population. Phagemids were plated, plasmid DNA purified, cDNA clones archived, and DNA sequences determined bi-directionally using an ABI3730 at the Arizona Genomics Institute, University of Arizona (Kim, Kudrna, Stum, Yost, Wing). Chromatogram files were downloaded by FTP to UC Riverside (by Close), then processed at UC Riverside (by Wanamaker, Close lab) using the HarvEST pipeline (http://harvest.ucr.edu) to remove vector and cloning oligo sequences and various contaminants, and to trim to a high quality region. Sequences that retained a phred 17 region of at least 100 bases were deposited to GenBank.

text: Commercially producing trees

characteristic: dev-stage

text: E. coli TJC121

text: Citrus sinensis

text: EcoRI

text: XhoI

text: Rind

text: Washington Navel Orange Stored Fruit Rind cDNA Library

text: Phagemid pBluescript SK(-)

text: Lambda Uni-ZAP XR, excised phagemid


text: Royal Institute of Technology, KTH

text: live

text: Generic

text: The cDNA libraries were created with the Superscript Plasmid System (Invitrogen).

text: ElectroMAX DH10B (Invitrogen)

text: brain

characteristic: organ

text: Gallus gallus

characteristic: organism

text: Hind III

text: EcoRI

text: female

characteristic: sex

text: Red junglefowl

text: RJbrain

text: plasmid


text: Royal Institute of Technology, KTH

text: live

text: Generic

text: ElectroMAX DH10B (Invitrogen)

characteristic: lab-host

text: brain

text: Gallus gallus

text: Hind III

text: EcoRI

text: female

text: White Leghorn

characteristic: strain

text: WLbrain

text: plasmid


characteristic: External Id

text: Royal Institute of Technology, KTH

text: live

text: Generic

characteristic: NCBI submission package

text: ElectroMAX DH10B (Invitrogen)

text: testis

text: Gallus gallus

text: Hind III

text: EcoRI

text: male

text: White Leghorn

text: WLtestis

text: plasmid


text: Royal Institute of Technology, KTH

characteristic: INSDC center name

text: live

text: Generic

text: ElectroMAX DH10B (Invitrogen)

text: testis

text: Gallus gallus

text: Hind III

text: EcoRI

text: male

text: Red junglefowl

text: RJtestis

text: plasmid


text: Department of pathology, Fujian Medical University

text: live

text: Generic

text: A subtracted cDNA library of differentially expressed genes in human gastric signet ring cell carcinoma is constructed with SSH and T/A clonning techniques.

text: stomach

text: Homo sapiens

text: female

text: human stomach signet ring cell carcinoma subtracted cDNA library

characteristic: title

text: Plasmid

text: pMD 18-T Vector


text: Cardiff University

text: live

text: Generic

text: A subtractive cDNA library was produced to identify genes that appear up-regulated in response to cadmium toxicity. Technique was performed using the Clontech PCR Select cDNA Subtraction Kit, using mRNA from control (unpolluted) and cadmium exposed (65.7ug/L) 54-day old rainbow trout.

characteristic: description

text: Oncorhynchus mykiss

text: Female

text: Upwey Seven-Spring

text: Cadmium exposed rainbow trout subtractive


text: Cardiff University

characteristic: INSDC first public

text: live

text: Generic

text: Gammarus pulex

text: XhoI

text: EcoRI

text: Mixed

text: mxA

text: pBluescript II SK+


text: Department of cell and biology

text: live

text: Generic

text: a cDNA expression library is constructed with pSPORT1.

text: adult

text: testis

text: Homo sapiens

text: male

text: seminiferous tubule

characteristic: tissue-type

text: Adult seminiferous tubule cDNA expression library

text: Plasmid


text: College of Animal Science, Zhejiang University

characteristic: INSDC last update

text: live

text: Generic

text: Bombyx mori

text: posterior silkgland

text: Silkworm posterior silkgland library

text: pBluescript II SK(+)


text: The Canadian Potato Genome Project - BioAtlantech

text: live

characteristic: INSDC status

text: Generic

text: Shepody

characteristic: cultivar

text: supplier: Developmental series. Callus was induced from Shepody, Clone 1756, sterile stem sections by culture on Callus Induction Medium (CIM), comprised of MS medium (pH 5.6) containing 10 mg/L Thiamine-HCL, .01 mg/L Kinetin and 3 mg/L 2,4-D solidified with .8% (w/v) Phytagar. Suspensions were induced by placing callus from the plates into 125 ml Erlenmeyer flasks with liquid CIM (no Phytagar) at a density of 10% (w/v) in volumes of approximately 30-35 ml. Cells were subcultured weekly by transfer to fresh media, with the density remaining at 10% (w/v) and the volume remaining around 30 ml. Cells were collected for RNA isolations and library construction 5 days after subculture.

text: Solanum tuberosum

text: EcoRI

text: XhoI

text: Callus-derived suspension culture

text: Suspension culture

text: pBluescript II SK(+) XR


text: INRA

text: live

text: Generic

characteristic: NCBI submission model

text: Vert d'Elne

text: Substractive library (SSH) enriched in cDNA coming from Apium graveolens phloem tissue and up-regulated by Myzus persicae infestation. This sequence has been generated in the framework of the French plant genomics program Genoplante (http://www.genoplante.org and http://genoplante-info.infobiogen.fr).

text: adult plant

text: Apium graveolens

text: phloem

text: BIP up-regulated phloem library after plant infestation with Myzus persicae

text: pCRII-TOPO

characteristic: vector


text: INRA

text: live

text: Generic

text: Vert d'Elne

text: Substractive library (SSH) enriched in cDNA coming from Apium graveolens phloem tissue and down regulated by Myzus persicae infestation. This sequence has been generated in the framework of the French plant genomics program Genoplante (http://www.genoplante.org and http://genoplante-info.infobiogen.fr).

text: adult plant

characteristic: dev-stage

text: Apium graveolens

text: phloem

text: BRP down-regulated phloem library after infestation with Myzus persicae

text: pCRII-TOPO


text: National Cancer Institute / NIH

text: live

text: Generic

text: 1st strand cDNA was primed with an oligo(dT) primer [GCGGCTGAAGACGGCCTATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded cDNA was ligated to a DraIII adaptor [GGCCUACUGG], digested and directionally cloned into distinct DraIII sites of the pME18S-FL3. Library was size selected for 1.0 kb, with a average insert size of ~1.2kb. Library constructed by Yutaka Suzuki (University of Tokyo Institute of Medical Science). Custom primers recommended for sequencing: 5' end primer 5'-GGATGTTGCCTTTACTTCTA-3' and 3' end primer 5'-CGACCTGCAGCTCGAGCACA-3'. Note: This is a Zebrafish Gene Collection (ZGC) library

text: DH10B TonA

text: mixed

characteristic: organ

text: Danio rerio

characteristic: organism

text: DraIII

text: DraIII

text: 5 day old whole larvae

text: phagemid


text: live

text: Generic

text: adult

text: heart

text: Rattus norvegicus

text: Adult rat heart cDNA library


text: University of Illinois at Urbana-Champaign

text: live

text: Generic

text: The cDNA library was constructed, normalized and subtracted by Dr. A. Hernandez, W.M. Keck Center, University of Illinois Urbana-Champaign and S. Degrelle, BDR, Institut National de la Recherche Agronomique, France, as described in Bonaldo, M.F., Lennon, G. and Soares, M.B. (1996), Genome Research 6(9): 791-806. This clone is from library BE01, a normalized library of embryonic tissues. The double stranded cDNA was size selected (more than 450 bp), adaptored with EcoRI adaptors at both ends and then digested with NotI. The cDNA was then directionally cloned into EcoRI-NotI digested pGEM11Zf(+) phagemid vector. Insert size was between 450-2200 bp. Sequencing was done from the 5' end of the clone. Tagged oligo-dT was used to identify the source of the ESTs where possible. (A18)TGCGT = extraembryonic tissue; (A18)TCGGT = 36-day old fetus; (A18)TGCGA = 64-day old fetus (organs); (A18)TCGGA = 64-day old fetus (body).

characteristic: lab-host

text: Bos taurus

text: EcoRI

text: NotI

text: embryo (day 36 and day 64) and extra-embryonic tissue (day 14 to 25)

text: Normalized and Subtracted bovine embryonic and extraembryonic tissue


text: Washington University School of Medicine

text: live

text: Generic

text: GoldRush

text: Total RNA was extracted separately from each floral stage (bud, balloon, open andafter pollination), using the 'pine tree' method. Poly(A)+mRNA was isolated twice from total RNA from each stage using the Oligotex Direct mRNA kit (Qiagen). mRNA was reverse transcribed into double stranded cDNA using a modified oligo18(dT) primer with an identifying tag sequence (see table below). cDNA's from different stages were pooled in equal amounts before adaptor ligation. Tag identification when sequencing from 5' end: Stage 1 (bud) insert 18(A)TCGGA; Stage 2 (balloon) insert 18(A)TGCGA; Stage 3 (open) insert 18(A)TGCGT; Stage 4 (afterpollination) insert 18(A)TCGGT. Tag identification when sequencing from 3' end: Stage 1 (bud) TCCGA18(T) insert; Stage 2 (balloon) TCGCA18(T) insert; Stage 3 (open) ACGCA18(T) insert; Stage 4 (afterpollination) ACCGA18(T) insert. Double stranded cDNAs were size selected (more than 450 bp), adaptored with EcoRI adapters at both ends and then digested with NotI. The cDNAs were then directionally cloned into EcoR1-NotI digested pBS II SK(+) phagemid vector(Stratagene). Identification of adaptors and tags in 5'-end sequenced clones: (Vector)...TAAGCTT(End Vector)(Start EcoR1_adaptor)GATATCGAATTCCATTGTGTTGGG (End EcoR1_adaptor)(Start Insert)....AAAAAAAAAAAAAAAAAA(End Insert) (Start Tag)TGCGA(End Tag)(Start NotI_site/Vector)GCGGCCGCCACCGCGG... The total number of white colony forming units (cfu) in the primary library beforeamplification was 1.1x10^6 cfu (colony forming units). The background of empty clones was less than 1%. Inserts ranged from 0.5kb to 3 kb, as determined by PCR. Purified plasmid DNA from the primary library was converted to single-strandedcircles and used as a template for PCR amplification using the T7 and T3 priming sites flanking the cloned cDNA inserts. The purified PCR products, representing the entire cloned cDNA population, were used as a driver for normalization. Hybridization between the single-stranded library and the PCR products was carried out for 44 hours at 30C. Unhybridized single-stranded DNA circles were separated from hybridized DNA rendered partially double-stranded and electroporated into DH10B cells to generate the normalized library. The total number of clones with insert was 9x10^6 cfu. Background of empty clones was less than 1%.

text: DH10B ampicillin resistant

text: Malus domestica

text: NotI

text: EcoRI

text: Flower

text: Mdfw

text: Phagemid (ampicillin resistant)

text: pBluescript II SK (+)


text: Northeast Agriculture University

text: live

text: Generic

text: leg

text: Gallus gallus

text: female

characteristic: sex

text: Binbai

characteristic: strain

text: BBleg

text: T18 vector


characteristic: External Id

text: University of Guelph

text: live

text: Generic

characteristic: NCBI submission package

text: Susceptible leaf tissue of Malva pusilla at 96 hours post inoculation with Colletotrichum gloeosporioides f. sp. malvae ATCC20767

text: Malva pusilla

text: Leaves

text: Lambda ZAP cDNA fungal infected round-leaved mallow library

text: phage

text: Lambda ZAP


text: Cold Spring Harbor Laboratory

characteristic: INSDC center name

text: live

text: Generic

text: Total cytosolic mouse RNA from : 7, 11, 15, & 17-day total embryo, whole brain, eye, kidney, liver, lung, prostate, submaxilary gland, smooth muscle, spleen, testes and uterus were divided into 4 pools with a maximum of 4 tissues/stages per pool. After 5' RACE cDNA generation, the cDNA for all the samples were combined and used in RACE-PCR amplification. Amplification of 5' RACE cDNA was carried out using nested gene-specific primers and adapter specific primers. After amplification, the products were cloned and sequenced from both ends using universal primers. 5'->3' cDNA sequence was artificially generated by joining the sequences from the ends for each clone.

text: Mus musculus

text: Pooled

text: 5' RACE cDNAs from cytosolic mouse RNA

characteristic: title

text: plasmid


text: Nanjing Forestry University

characteristic: INSDC first public

text: live

text: Generic

text: Lycoris longituba

text: EcoRI

text: XhoI

text: flower bud

text: Flower bud cDNA library from Lycoris longituba

text: Phage

text: ZAP expression vector


text: live

text: Generic

text: The library was constructed according Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. Denatured RNA was size fractionated on a 1% agarose gel.First strand cDNA synthesis was primed with oligo-dT primer containing a Not I site .Double strand cDNA was size selected according to mRNA size fraction, ligated with EcoR I adaptor, digested with NotI and then cloned directionally into pYX-Asc vector. The library tag sequence located between the Not I site and the polyA tail is CTGCGTCCTC. This library was created for the University Iowa Brain Anatomy Project (BMAP): 'Gene Discovery in the Developing Mouse Nervous System', supported by National Institute of Mental Health (NIMH).

characteristic: description

text: embryo 15.5,16.5,17.5,18.5 dpc

text: DH10B (T1 phage resistant)

text: Eye

text: Mus musculus

text: EcoR I

text: Not I

text: whole eye

characteristic: tissue-type

text: Plasmid

text: pYX-Asc


text: The Canadian Potato Genome Project - BioAtlantech

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Shepody

text: supplier: Developmental series. Plants from pathogen-free Solanum tuberosum var. Shepody, clone 1756, nuclear stock were grown in a screenhouse under natural conditions. Mature, harvested tubers were stored in the dark at 9C, 95% relative humidity for 1 month. The tuber skin was then removed, and RNA was isolated from the skinless tubers and used for library construction.

text: Solanum tuberosum

text: EcoRI

text: XhoI

text: Tubers

text: After-Cooking Darkening A

text: pBluescript II SK(+) XR


text: Chungnam National University

text: live

text: Generic

characteristic: NCBI submission model

text: Seeds of Leymus chinensis were germinated at 4oC under dark conditions and seedlings were grown under greenhouse conditions (maximum light intensity of 1200 umol s-1 m-2 and temperature ranges from 21oC night to 32oC day). Two-month-old plants were treated with 100 mM Na2CO3, pH 11.4 for 48 hours, and roots were harvested and stored at -70oC until RNA extraction for cDNA construction. The mRNA was selected with PolyATtract mRNA Isolation Systems III (Promega). The cDNA was ligated unidirectionally into the EcoRI and XhoI site of the lambda-ZAP vector (Stratagene).

text: 2 months old plant

text: Leymus chinensis

text: EcoRI

text: XhoI

text: Root

text: Leymus chinensis root cDNA library

text: lambda-ZAP


text: CNRS-LPR9042, Universite P. & M. Curie

text: live

text: Generic

text: protoplasts

characteristic: cell type

text: Laminaria digitata

text: Eco R1

text: Laminaria digitata protoplasts Zap Express Library

text: pbK-CMV

characteristic: vector


text: University of Padova

text: live

text: Generic

text: Fantasia

characteristic: cultivar

text: S4 climacteric

characteristic: dev-stage

text: Prunus persica

text: mesocarp

text: Prunus persica mesocarp S4 climacteric


text: King's College London, GKT School of Medicine

text: live

text: Generic

text: Homo sapiens

characteristic: organism

text: testes

text: Homo sapiens testes


text: INRA - SCRIBE

text: live

text: Generic

text: Clone distribution : AGENAE Resource centre. Francois PIUMI, Francois.Piumi@jouy.inra.fr, INRA, CEA Radiobiologie et Etude du genome (LREG), Domaine de Vilvert, 78352, Jouy-en-Josas cedex, FRANCE, +33 (0) 1.34.65.28.02, +33 (0) 1.34.65.22.73

characteristic: lab-host

text: Oncorhynchus mykiss

text: ovaries

text: AGENAE Rainbow trout normalized ovarian library (tcby)


text: Harvard University

text: live

text: Generic

text: mRNA was isolated from the brains of A. burtoni for both sexes at all stages of development and reproductive status.

text: mixed

text: XL1-Blue MRF' E.coli

text: Haplochromis burtoni

text: mixed

characteristic: sex

text: brain

text: A. burtoni Cichlid Brain cDNA library 2000, Russell Fernald

text: plasmid

text: pBSIIsk-


characteristic: External Id

text: USDA-ARS

text: live

text: Generic

text: A high quality EST with at least 200 contiguous bases at Trace Tuner score of 20 or better. Construction by PM DANG and ANNA SARA HILL, USDA, ARS, U.S. Horticultural Res. Lab, Ft. Pierce, FL, USA.

text: Teneral

text: Diaprepes abbreviatus

text: EcoRI

text: XhoI

text: Adult Female

text: Whole body

text: Diaprepes abbreviatus, Diaprepes root weevil

text: pBluescript II SK+


text: Institute of Plant Physiology of Shanghai Institutes for Biological Sciences, The Chinese Acadamy of Sciences

text: live

text: Generic

characteristic: NCBI submission package

text: 10-14 days by shaking culture

characteristic: isolate

text: Mycelium

characteristic: organ

text: Verticillium dahliae

text: Verticillium dahliae Mycelium Lambda Zap Express Library

text: Phage


text: Wuhan University

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: ecotype: biotype II

text: Nilaparvata lugens

text: Nilaparvata lugens biotype II

characteristic: title


text: Museum National d'Histoire Naturelle

characteristic: INSDC last update

text: live

text: Generic

text: schizont

text: Plasmodium falciparum

characteristic: strain

text: Plasmodium falciparum FCB1 schizont


text: Forage Crop Research Institute(FCRI)

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Lolium multiflorum

text: Callus

characteristic: tissue-type

text: CA


text: Forage Crop Research Institute(FCRI)

text: live

text: Generic

text: Lolium multiflorum

text: Cold shock leaf

text: CL


text: Forage Crop Research Institute(FCRI)

text: live

text: Generic

text: Lolium multiflorum

text: Inflorescence

text: FL


text: Forage Crop Research Institute(FCRI)

text: live

text: Generic

text: Lolium multiflorum

text: Leaf

text: LE


text: Forage Crop Research Institute(FCRI)

text: live

text: Generic

text: Lolium multiflorum

text: Powdery mildew infected leaf

text: SL


text: Forage Crop Research Institute(FCRI)

text: live

text: Generic

text: Lolium multiflorum

text: Stem

text: ST


text: Forage Crop Research Institute(FCRI)

text: live

text: Generic

text: Lolium multiflorum

text: Heat shock leaf

text: HL (Lolium multiflorum)


text: Kyungpook National University School of Medicine

text: live

text: Generic

text: adult

text: XL1-Blue MRF

text: Paragonimus westermani

text: EcoRI

text: XhoI

text: hermaphrodite

text: Paragonimus westermani 3N cDNA library

text: pBluescript SK

characteristic: vector


text: Kagawa University

text: live

text: Generic

text: Citrus jambhiri

text: leaf

text: full-length enriched rough lemon cDNA library


text: National Institute of Agrobiological Sciences

text: live

text: Generic

text: 28 day-old embryo

characteristic: dev-stage

text: Sus scrofa

text: embryo

text: 28 day-old swine embryo cDNA


text: c/o UniMi DiProVe, Parco Tecnologico Padano

text: live

text: Generic

text: tissue_lib: S3 ; country: Italy

characteristic: description

text: final maturation steps, pre-climateric

text: Prunus persica

characteristic: organism

text: fruit mesocarp

text: Prunus persica fruit mesocarp final maturation steps, pre-climateric


text: live

text: Generic

text: tissue_lib: S4 ; country: Italy

text: final maturation steps, post-climateric

text: Prunus persica

text: fruit mesocarp

text: Prunus persica fruit mesocarp final maturation steps, post-climateric


text: National Eye Institute

text: live

text: Generic

text: pericytes

characteristic: cell type

text: RNA was extracted from primary human pericytes in culture. A directionally cloned cDNA library in the pSPORT1 vector (Invitrogen) was constructed at Bioserve Biotechnology (Laurel MD) essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. cDNA was cloned in Not I/Sal I sites. EST analysis was performed at the NIH Intramural Sequencing Center (NISC).

text: Adult

characteristic: lab-host

text: Eye

text: Homo sapiens

text: Human primary human ocular pericytes. Unamplified (hw)

text: Plasmid


characteristic: External Id

text: National Eye Institute

text: live

text: Generic

characteristic: NCBI submission package

text: pericytes

text: RNA was extracted from primary human pericytes in culture. A directionally cloned cDNA library in the pSPORT1 vector (Invitrogen) was constructed at Bioserve Biotechnology (Laurel MD) essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. cDNA was cloned in Not I/Sal I sites. EST analysis was performed at the NIH Intramural Sequencing Center (NISC). This library is derived from the original unamplified library and subjected to equalization to reduce occurrence of abundant clones.

text: Adult

text: Eye

text: Homo sapiens

text: Human primary human ocular pericytes. Equalized (hx)

text: Plasmid


text: Northeast Forestry University

text: live

text: Generic

text: Puccinellia tenuiflora

text: LEAF

text: Puccinellia tenuiflora cDNA library


text: Institute of Plant Science

text: live

text: Generic

text: Eight-day seedlings

text: Oryza sativa

text: root

text: Water-deficit induced fragments in three parts of rice roots

text: PUCm-T


text: Dipartimento di Biologia Vegetale, Universita degli Studi di Torino

text: live

text: Generic

text: cDNA library from mature fruit bodies of Tuber borchii, field collected. The cDNA library was constructed from 1 ug of total RNA using the SMART cDNA synthesis kit (Clontech, Palo Alto, CA, USA) according to the manufacturer's instructions. The resulting cDNA was packed into lambda phages using the Gigapack III Gold packaging kit (Stratagene, La Jolla, CA). The pTriplEx2 phagemid clones in Escherichia coli were obtained by using the mass in vivo excision protocol according to the manufacturer's instructions (Clontech).

text: sexual stage

text: Tuber borchii

text: fruit body

text: Tuber borchii mature fruit body library

text: Phagemid (Stratagene,USA)


text: live

text: Generic

text: cDNA library from immature fruit body of Tuber borchii, field collected. The cDNA library was constructed from 1 ug of total RNA using the SMART cDNA synthesis kit (Clontech, Palo Alto, CA, USA) according to the manufacturer's instructions. The resulting cDNA was packed into lambda phages using the Gigapack III Gold packaging kit (Stratagene, La Jolla, CA). The pTriplEx2 phagemid clones in Escherichia coli were obtained by using the mass in vivo excision protocol according to the manufacturer's instructions (Clontech).

text: sexual stage

text: Tuber borchii

text: fruit body

text: Tuber borchii immature fruit body library

text: Phagemid (Stratagene,USA)


text: University of Bath

text: live

text: Generic

text: Diabrotica virgifera virgifera cDNA library from mRNA extracted from dissected Diabrotica midguts.

text: Diabrotica virgifera virgifera

text: midguts

text: Diabrotica virgifera virgifera midguts


text: Kansas State University

text: live

text: Generic

text: Suppression subtractive hybridization; Cloned in pBluescript.

text: 8 week-old plants

text: Nicotiana tabacum

text: W38 carrying avrPto

text: Leaves

text: Tobacco leaves expressing AvrPto

text: pBluescript


text: University of Edinburgh

text: live

text: Generic

text: adult

text: Diplosoma listerianum

text: mixed

characteristic: sex

text: Diplosoma listerianum mixed adult cDNA library


text: The Canadian Potato Genome Project - BioAtlantech

characteristic: INSDC center name

text: live

text: Generic

text: Shepody

characteristic: cultivar

text: supplier: Developmental series. Plants from pathogen-free Solanum tuberosum var. Shepody, clone 1756, nuclear stock were grown in a screenhouse under natural conditions. Tubers were harvested from plants at the time of top kill, as well as 2 weeks post top kill. Tuber skin was peeled from these tubers at the time of collection and frozen with liquid nitrogen. RNA was isolated from tuber skin at each collection, and pooled for use in library construction.

text: Solanum tuberosum

text: EcoRI

text: XhoI

text: Tuber Skin

text: Tuber Skin

text: pBluescript II SK(+) XR


characteristic: INSDC first public

text: live

text: Generic

text: The library was constructed according Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. Denatured RNA was size fractionated on a 1% agarose gel.First strand cDNA synthesis was primed with oligo-dT primer containing a Not I site .Double strand cDNA was size selected according to mRNA size fraction, ligated with EcoR I adaptor, digested with NotI and then cloned directionally into pYX-Asc vector. The library tag sequence located between the Not I site and the polyA tail is CGAACTGAAT. This library was created for the University Iowa Brain Anatomy Project (BMAP): 'Gene Discovery in the Developing Mouse Nervous System', supported by National Institute of Mental Health (NIMH).

text: embryo 9.5 - 10.5 dpc

text: DH10B (T1 phage resistant)

text: Upper Head

characteristic: organ

text: Mus musculus

text: EcoR I

text: Not I

text: Upper Head

characteristic: title

text: Plasmid

text: pYX-Asc


characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: The library was constructed according Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. Denatured RNA was size fractionated on a 1% agarose gel.First strand cDNA synthesis was primed with oligo-dT primer containing a Not I site .Double strand cDNA was size selected according to mRNA size fraction, ligated with EcoR I adaptor, digested with NotI and then cloned directionally into pYX-Asc vector. The library tag sequence located between the Not I site and the polyA tail is AATAATTACG. This library was created for the University Iowa Brain Anatomy Project (BMAP): 'Gene Discovery in the Developing Mouse Nervous System', supported by National Institute of Mental Health (NIMH).

text: newborn( 1, 5, 15 days )

text: DH10B (T1 phage resistant)

text: Eye

text: Mus musculus

text: EcoR I

text: Not I

characteristic: strain

text: whole eye

text: Plasmid

text: pYX-Asc


text: live

text: Generic

characteristic: NCBI submission model

text: newborn (1, 5, 15 days )

text: DH10B (T1 phage resistant)

text: Eye

text: Mus musculus

text: EcoR I

text: Not I

text: whole eye

characteristic: tissue-type

text: Plasmid

text: pYX-Asc

characteristic: vector


text: University of Maryland College Park

text: live

text: Generic

text: Gala

text: immature

characteristic: dev-stage

text: Malus domestica

characteristic: organism

text: Hypanthium tissue

text: Apple developing fruit differentially expressed cDNA library


text: West Virginia University

text: live

text: Generic

text: oocyte

characteristic: cell type

text: Poly (A)+ RNA isolated from a pool of 100 germinal vesicle stage and 100 metaphase II stage oocytes was used for construction of the library using a PCR-based cDNA amplification method (SMART technology)

characteristic: description

text: germinal vesicle and metaphase II

characteristic: lab-host

text: Bos taurus

text: NotI

text: SalI

text: female

text: Bovine oocyte cDNA Library

text: Plasmid


characteristic: External Id

text: Universite de Bordeaux I, Institut National de la Recherche Agronomique

text: live

text: Generic

characteristic: NCBI submission package

text: Cabernet Sauvignon

text: Oriented library

text: green stage

text: Fruit skin

text: Vitis vinifera

text: SfiIA

text: SfiIB

text: Green Grape Skin Triplex2 Library

text: Phage

text: Lambda TriplEx2


characteristic: INSDC center name

text: live

text: Generic

text: Cabernet Sauvignon

characteristic: cultivar

text: Oriented library

text: ripening stage

text: Fruit skin

text: Vitis vinifera

text: SfiIA

text: SfiIB

text: Ripe Grape Skin Triplex2 Library

text: Phage

text: Lambda TriplEx2


text: Washington University School of Medicine

characteristic: INSDC first public

text: live

text: Generic

text: (pGEM-T Vector( from Promega) is ampicillin resistent, M13 forward and M13 reverse, or SP6 primers should be used for sequencing. Because of the construction peculiarities T7 primer can not be used for sequencing.

text: Hydra vulgaris

text: EcoRI

text: EcoRI

text: AEP

text: Hydra EST -Kiel 3

text: plasmid

text: pGEM-T


text: University of California

text: live

text: Generic

text: debladed plant

text: young plant

text: Mirabilis jalapa

text: petiole abscission zone

text: Mirabilis jalapa petiole abscission zone young plant

characteristic: title


text: University of Padua

characteristic: INSDC last update

text: live

text: Generic

text: Samsung NN

text: Nicotiana tabacum

text: leaf

text: Nicotiana tabacum leaf


text: Univ. Saarland

text: live

characteristic: INSDC status

text: Generic

text: Homo sapiens

text: testis

text: Homo sapiens testis


text: INRA, Genomique & Physiologie de la Lactation

text: live

text: Generic

text: Clone distribution : AGENAE Resource centre. Francois PIUMI, Francois.Piumi@jouy.inra.fr, INRA, CEA Radiobiologie et Etude du genome (LREG), Domaine de Vilvert, 78352, Jouy-en-Josas cedex, FRANCE, +33 (0) 1.34.65.28.02, +33 (0) 1.34.65.22.73

text: from embryos to adults

text: Bos taurus

text: muscles : heart, longissimus thoracis, semitendinosus, masseter, cutaneus trunci

text: Bovine multi-stage muscles library (bcaj)


text: live

text: Generic

text: from embryos to adults

text: Bos taurus

text: Bovine multi-stage muscles library (bcas)


text: Dalhousie University

text: live

text: Generic

text: Prototheca wickerhamii

text: EcoRV

text: Not I

text: SAG 263-11

characteristic: strain

text: Prototheca wickerhamii library

text: pcDNA3.1 vector (Invitrogen, Carlsbad, CA)


text: BioPia

text: live

text: Generic

characteristic: NCBI submission model

text: Chunpoong

text: country: South Korea: Chungnam, Yeongi-gun, Bongam-li

text: Panax ginseng

text: root

text: lambda ZAPII ginseng root cDNA


text: Rothamsted Research,

text: live

text: Generic

text: Library constructed from cultures grown in Czapek Dox for 3 days then Glucose Yeast Nitrogen Base for 1 day, shift of nitrogen source from nitrate to ammonium ions

text: Zymoseptoria tritici

text: Strit

text: MgA


text: Rothamsted Research,

text: live

text: Generic

text: Library constructed from cultures grown in Czapek Dox for 3 days then grown in media containing a soluble extract of green wheat leaves for 1 day

text: Zymoseptoria tritici

text: Strit

text: MgB


text: Rothamsted Research,

text: live

text: Generic

text: Library constructed from senescent wheat leaves 21-25 days after infection with Mycosphaerella graminicola exhibiting abundant hyphal growth and asexual sporulation

text: Zymoseptoria tritici

text: Strit

text: MgC


text: Departement de Biochimie, Universite de Montreal

text: live

text: Generic

text: Tetrahymena thermophila

text: Non-normalized


text: live

text: Generic

text: Tetrahymena thermophila

text: Normalized large


text: live

text: Generic

text: Tetrahymena thermophila

text: Normalized small


text: Institute of Physiology

text: live

text: Generic

text: Gallus gallus

text: Gallus gallus EST sequence


text: South Dakota State University

text: live

text: Generic

text: VRK is a cDNA library of Vitis riparia paradormant buds. Compound paradormant buds were collected from actively growing shoots on insect and disease free vines. The directionally oriented library was constructed according to Gibco BRL Superscript Plasmid System for cDNA synthesis and plasmid cloning.

text: paradormant

text: bud

characteristic: organ

text: Vitis riparia

text: SalI

text: NotI

text: Vitis riparia bud - VRK

text: pSport 1


text: University College London

text: live

text: Generic

text: embryo stage 3+/4

text: Gallus gallus

text: hensens node

characteristic: tissue-type

text: embryo stage 3+/4 (hensens node)

text: Lambda Zap

characteristic: vector


text: University College London

text: live

text: Generic

text: embryo stage 2-4

characteristic: dev-stage

text: Gallus gallus

characteristic: organism

text: EcoR1 (5')

text: Xho1 (3')

text: embryo stage 2-4

text: Lambda Uni-ZapXR


text: deCode Genetics

text: live

text: Generic

text: Fetal

text: Brain

text: Homo sapiens

text: female and male

characteristic: sex

text: Marathon-Ready cDNA-Clontech - Fetal brain


text: deCode Genetics

text: live

text: Generic

text: Adult

text: Brain

text: Homo sapiens

text: female and male

text: Hippocampus

text: Marathon-Ready cDNA-Clontech - Hippocampus


text: deCode Genetics

text: live

text: Generic

text: Adult

text: Brain

text: Homo sapiens

text: female and male

text: Hypothalamus

text: Marathon-Ready cDNA-Clontech - Hypothalamus


text: deCode Genetics

text: live

text: Generic

text: Adult

text: Brain

text: Homo sapiens

text: female and male

text: Marathon-Ready cDNA-Clontech - Brain


text: deCode Genetics

text: live

text: Generic

text: Made from Clontech total brain RNA

characteristic: description

text: Adult

text: Brain

text: Homo sapiens

text: female and male

text: Inhouse


characteristic: External Id

text: Graduate School of Science, University of Tokyo; CREST, Japan Science and Technology Corporation, Japan

text: live

text: Generic

text: late gastrula (stage 12.5)

text: Xenopus laevis

text: anterior neuroectoderm

text: Osada Taira anterior neuroectoderm (ANE) pCS105 cDNA library


text: Northeast Forestry University

text: live

text: Generic

characteristic: NCBI submission package

text: Tamarix androssowii

text: LEAF

text: cDNA library of Tamarix androssowii treated with NaHCO3


text: Tianjin Endocrinology Institute

characteristic: INSDC center name

text: live

text: Generic

text: cDNA fragment related to the skeletal muscle of hyperthyroid hormonal rat

text: Rattus norvegicus

text: skeletal muscle

text: Wister rat skeletal muscle


text: The Chinese University of Hong Kong

text: live

text: Generic

text: Vegetative mycelium strain L54 was cultivated in sawdust medium. Primordium was stimulated by induction of cold shock. mRNA from primordium was extracted. The subsequently synthesized cDNA library was cloned into ZAP Express(TM) vector(Stratagene).

text: Primordium

text: Lentinula edodes

text: EcoRI

text: EcoRI

text: Dikaryon

text: Primordial expressed cDNA library lambda ZAP

text: Phage

text: lambda ZAP Express(TM) vector (Stratagene)


text: The Chinese University of Hong Kong

text: live

text: Generic

text: Vegetative mycelium strain L54 was cultivated in sawdust medium. Primordium was stimulated by induction of cold shock. mRNA from primordium was extracted. The subsequently synthesized cDNA library was cloned into lambda TriplEx2 Vector (Clontech).

text: Primordium

text: Lentinula edodes

text: SrfI

text: SrfI

text: Dikaryon

text: Primordial expressed cDNA library lambda TriplEx2

text: Phage

text: lambda TriplEx2 Vector (Clontech)


text: SiChuan Agricultrual University

text: live

text: Generic

text: Adult

characteristic: lab-host

text: Liver

text: Gallus gallus

text: Black-bone Chicken's Liver Differential Express Library

text: pBluescript-T


text: INRA - INSA de Lyon

characteristic: INSDC first public

text: live

text: Generic

text: Bacteriocyte

characteristic: cell type

text: Sized cDNAs were directly ligated into pCR2.1TOPO

text: Fourth instar larvae

text: E. coli TOPO10

text: Bacteriome

text: Sitophilus zeamais

text: EcoRI

text: BstXI

text: Lagoa (origin Azores)

text: Weevil Bacteriocyte Subtracted Library


text: Agricultural Research Service

characteristic: INSDC last update

text: live

text: Generic

text: epithelial

text: adult, 2-4 day-old

text: Culicoides sonorensis

text: female

text: AK

text: salivary gland

text: Culicoides sonorensis female salivary gland cDNA Library

characteristic: title


text: Institute of Forest Genetics, USDA Forest Service, Pacific Southwest Research Station

text: live

characteristic: INSDC status

text: Generic

text: The cDNA library was obtained from one month old Douglas-fir seedlings. Total RNA was extracted from ~5 g of ground tissue that consisted of the mixture of 10 seedlings following the protocol described in Chang et al. (1993). mRNA was isolated from the total RNA using the PolyATract mRNA Isolation System III (Promega Z5300). Double stranded cDNA was prepared using the Universal RiboClone cDNA Synthesis System (Promega C4360) and filtered through a Sephacryl S-400 column to remove low molecular weight products of less than 300 bp. Double-stranded blunt-end cDNA was ligated into the EcoICRI-cut dephosphorylated pGEM-3Z sequencing vector and electroporated into E. coli DH5 alpha. cDNA clones were sequenced by Integrated Genomics, Inc. (Chicago, USA).

text: one month old seedlings

text: Pseudotsuga menziesii var. menziesii

text: EcoICRI

text: EcoICRI

text: entire seedlings

text: Douglas-fir cDNA library PmIFG_73-6

text: Plasmids


text: National Center for Genetic Engineering and Biotechnology

text: live

text: Generic

characteristic: NCBI submission model

text: Total RNA was isolated from branchiae of Penaeus monodon. The mRNA was further purified, reverse transcribed, blunt-ends filled and cloned to SmaI-cut pBluescript Vector.

text: 3 months old

text: E. coli DH10B

text: Penaeus monodon

text: branchiae

text: PmBr cDNA Library

text: phagemid

text: pBluescript II SK(+/-)


text: The Sanger Institute The Wellcome Trust Genome Campus

text: live

text: Generic

text: Echinococcus granulosus is a cestode parasite of dogs (definitive host) and various domestic and wild animals as well as humans (intermediate hosts). The library was constructed from the wall (germinative and laminated layers) obtained from a hydatid cyst present in the lungs of a naturally infected Uruguayan bovine. cDNA was prepared by RT-PCR and directionally cloned in a plasmid vector. RT was primed with a tagged oligo-dT (NotI primer-adapter). This tag was then used to prime cDNA amplification together with a primer directed against an oligonucleotide previously ligated to the originally capped RNA population. cDNA was ligated to SalI adapters, digested with NotI, and cloned into pSPORT 1 cut with SalI and NotI. This sequence has been identified as originating from the host (Bos taurus).

text: Bos taurus

text: SalI (5'end)

text: NotI (3'end)

text: Bos taurus - Echinococcus granulosus germinative layer (full length enriched)


text: live

text: Generic

text: Echinococcus granulosus is a cestode parasite of dogs (definitive host) and various domestic and wild animals as well as humans (intermediate hosts). The library was constructed from protoscoleces obtained from hydatid cysts present in the lungs of naturally infected Uruguayan bovines. cDNA was prepared by RT-PCR and directionally cloned in a plasmid vector. RT was primed with a tagged oligo-dT (NotI primer-adapter). This tag was then used to prime cDNA amplification together with a primer directed against an oligonucleotide previously ligated to the originally capped RNA population. cDNA was ligated to SalI adapters, digested with NotI, and cloned into pSPORT 1 cut with SalI and NotI. This sequence has been identified as originating from the host (Bos taurus).

text: Bos taurus

text: SalI (5'end)

text: NotI (3'end)

text: Bos taurus - Echinococcus granulosus protoscolex (full length enriched)


text: University of Missouri-Columbia

text: live

text: Generic

text: Funding: The production of ESTs submitted in this project was funded by USDA Grant MRI-2002-03476 entitled 'Bovine ESTs: Focus on Female Reproduction' to RS Prather(Primary Investigator), E Antoniou, HA Garverick, JA Green, MC Lucy, RM Roberts, MF Smith and RS Youngquist. Genetic Source: Heifers for the project were purchased from Circle A Ranch, Iberia, MO (http://www.circlearanch.com/home.html). These heifers, while not registered have known Angus pedigrees going back at least 4 generations. Samples collected: The samples consisted of the following: germinal vesicle-stage oocytes; in vitro derived embryos (2-cell, morula, blastocyst and nuclear transfer blastocyst); in vivo blastocysts and conceptuses (days 8, 14, 16 and 18); corpora lutea (days 3, 5, 8, 14, 16, 18 and 35); ovarian follicles (days 0, non-recruited, recruited, early selected and preovulatory); oviduct (days 0, 3 and 5); endometrium (days 5, 8, 14, 16, 18 and 35); and placenta/embryo from day 35 conceptuses. Expanded descriptions of how the tissues were collected can be found at the following URL: http://genome.rnet.missouri.edu/Bovine/Methods.html. Library construction (Standard Protocol): All procedures have been described in detail elsewhere (Soares et al., 1994; Bonaldo et al., 1996; Jiang et al., 2001). Total cellular RNA from each sample was isolated by using STAT-60 reagent (Tel-Test, Friendswood, TX) and the poly(A)+ RNA was obtained by two rounds of purification with the Oligotex mRNA isolation kit (Qiagen) according to the manufacturer's instructions. The libraries were constructed essentially as described by the manufacturer's instructions provided with the SuperScript Plasmid System (Invitrogen, cat. no. 18248-013). Briefly, 1mcg of poly(A)+ RNA was annealed at c37 degrees with 10mcg of NotI-tag-dT18 oligonucleotide (GCTGCTCGCGGCCGC-tag-T18) and reverse transcribed at c37 degrees with SuperScript II (Invitrogen) reverse transcriptase (Jiang et al., 2001). The 'tag' represents a tissue/stage-specific ten-base sequence identifier (http://genome.uiowa.edu/pubsoft/software.html) present in the oligonucleotide used to prime first-strand synthesis. Second strand synthesis was performed with T4 DNA polymerase in the presence of DNA ligase and RNase H. After second strand synthesis, the double-stranded cDNAs were ligated to SalI adapters (Invitrogen-Life Technologies) and digested with NotI. The cDNAs were size selected by passage through cDNA size fractionation columns (Invitrogen-Life technologies). The cDNAs derived from each developmental stage of a particular tissue were mixed on an equimolar basis and ligated directionally into the NotI and SalI sites of the pCMV-SPORT6 vector (Invitrogen). After ligation of the inserts, the plasmids were electroporated into DH10B bacteria. Library Construction (PCR Protocol): The amount of mRNA that was recovered from oocytes and embryos was quite limiting and was not sufficient for library production with the standard protocol. Therefore, PCR-based protocol was utilized for producing libraries from sources in which the amount of extracted mRNA was small (oocytes and embryos). Poly-A RNA was isolated by using the MicroPoly(A) Pure kit from Ambion (cat. # 1918). The mRNA was reverse transcribed with a NotI-tag-dT18 oligonucleotide and a SMART oligonucleotide (Clontech) modified to contain a SalI site to generate full-length cDNA with a sequence complementary to the SMART oligonucleotide. Sequences within the SMART and dT oligonucleotides were used as primers to amplify the cDNAs by PCR with pfu turbo polymerase (Stratagene). The resulting PCR products were purified, digested with NotI and SalI and size fractionated by using Chroma Spin-1000 columns (Clontech). Purified cDNA from each PCR reaction was quantitated and mixed on an equimolar basis for ligation into the pCMV- SPORT6 vector. Preliminary Library Characterization: Randomly chosen clones from each library were analyzed by restriction digestion to determine average insert size (96 clones) and by sequencing (~4 96-well plates) to confirm library quality [e.g. the presence of short polyA+ tails, genomic DNA contamination (must be <1%), ribosomal RNA clones (must be <1%), etc.] and to provide a sequence database representing the predominant clones in each library. The clones were sequenced at the University of Missouri-Columbia DNA Core Facility. After production of the libraries, equal numbers of recombinants from each library were pooled to produce a single mixed library (mega-library) for more extensive sequencing. Bioinformatics work was performed by GK Springer's bioinformatics group in Computer Science at the University of Missouri-Columbia. Clone Requests: Requests for clones should be made to the Director of the University of Missouri DNA Core facility at: bovine@rnet.missouri.edu. Bonaldo MF, Lennon G, Soares MB,. Normalization and Subtraction: Two approaches to facilitate gene discovery. Genome Res, 1996; 6:791-806. Jiang H, Bivens NJ, Ries JE, Whitworth KM, Green JA, Forrester LJ, Springer GK, Didion BA, Mathialagan N, Prather RS, Lucy MC (2001) Constructing cDNA libraries with fewer clones that contain long poly(dA) tails. Biotechniques 31:38-42. Soares MB, MF Banaldo, P Jelene, L Su, L Lawton, A Efstrantiadis. 1994. Construction and characterization of a normalized cDNA library. Proc Natl Acad Sci, 91:9228-9232.

text: Bos taurus

text: bcl


text: North Carolina State University

text: live

text: Generic

text: Berkeley

characteristic: cultivar

text: Total RNA isolated from tissue, poly A fraction isolated on oligo-dT column. mRNA reverse transcribed and double stranded. Poly A dsRNA ranging in size from 400-4000 bp directionally cloned into EcoR1 and Xho1 restricted into pBluescript. EcoR1 site at the 5' end of the cDNA and Xh01 on the 3' side.

text: Nicotiana benthamiana

text: expanding leaf tissue

text: SAL_US N.Bentheme

text: plasmid

text: pBluescript S/K +


text: US Department of Agriculture (USDA), ARS, PSI

text: live

text: Generic

text: Kent

text: cDNA clones from mRNA extracted from syncytium of soybean roots cv. Kent after infection by SCN race 3.

text: Seedlings

text: Glycine max

text: Roots

characteristic: tissue-type

text: LCM003 library

text: phagemid

text: pCR4 TOPO


text: Washington University School of Medicine

text: live

text: Generic

text: The library was constructed by Irina Ronko and Dr. Makedonka Mitreva (mmitreva@watson.wustl.edu) at Washington University, St. Louis. Oligo(dT)-SL2b PCR based library. Pristionchus pacificus mixed stage cDNA PCR products of size >400 nucleotides containing SL2b (GGTTTTAACCCAGTATCTCAAG) on the 5' end and oligo(dT) on the 3' end were non-directionally cloned into pCRII-TOPO(Invitrogen) following the Topo TA cloning protocol.

text: Mixed

text: Pristionchus pacificus

text: 5': EcoRI

text: 3': EcoRI

text: Pristionchus pacificus mixed stage SL2b TOPO v1

text: plasmid (kanamycin and ampicillin resistant)

text: pCRII-TOPO (Invitrogen)

characteristic: vector


text: live

text: Generic

text: The cDNA library was constructed by Keliang Tang, and Robert Cole at Washington University. Total RNA was converted to cDNA using the template-switching PCR method (Creator SMART cDNA, Clontech Inc.). First strand was PCR amplified using the same primer set and the fragments were digested with SfiI. The fragments were size selected, ligated into vector pDNR_LIB containing directional SfiI sites, and electroporated into GC10 competent cells. WARNING: the library contains a small percentage of cDNAs derived from the human host cells.

text: Tachyzoite

characteristic: dev-stage

text: Toxoplasma gondii

text: SfiI

text: SfiI

text: Tg CAST Tachyzoite cDNA Library 2

text: pDNR-LIB


text: National Institute on Aging/National Institutes of Health

text: live

text: Generic

text: Mouse cDNA project by the Laboratory of Genetics, National Institute on Aging (NIA), Intramural Research Program, NIH (http://lgsun.grc.nia.nih.gov/cDNA). This is a long-transcript enriched cDNA library (Ref. Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]). Total RNAs were obtained from Drs. Dennis Taub, Dan Longo (National Institute on Aging, USA), Jonathan Keller (National Cancer Institute, USA). Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 4.8 ug of total RNA, treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-S. The products were purified by phenol/chloroform and Centricon 100. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pCMV-SPORT6 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 2.7 kb. The library was constructed by Yulan Piao.

text: Age ~10 weeks old

text: Mus musculus

characteristic: organism

text: SalI

text: NotI

characteristic: strain

text: NIA Mouse Hematopoietic Stem Cell (Lin-/c-Kit+/Sca-1+) cDNA Library (Long 1)

text: plasmid (ampicillin resistant)

text: pCMV-SPORT6 (Invitrogen)


text: live

text: Generic

text: Mouse cDNA project by the Laboratory of Genetics, National Institute on Aging (NIA), Intramural Research Program, NIH (http://lgsun.grc.nia.nih.gov/cDNA). This is a long-transcript enriched cDNA library (Ref. Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]). Total RNAs were extracted from 1 embryo at 13.5-days postcoitum. Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 3ug of total RNA, treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-S. The products were purified by phenol/chloroform and Centricon 100. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pCMV-SPORT6 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 3.0Kb. The library was constructed by Yulan Piao.

characteristic: description

text: Mus musculus

text: SalI

text: NotI

text: whole embryo including extraembryonic tissues at 13.5-days postcoitum

text: NIA Mouse E13.5 whole embryo cDNA library (Long)

text: plasmid (ampicillin resistant)

text: pCMV-SPORT6 (Invitrogen)