text: Cancer Research Institute

text: live

text: Generic

text: The mRNA from the liver of Andrias davidianus was purified and the first-strand cDNA was synthesized by reverse transcription with SMART Oligonucleotide (5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTATGGCCGGG-3') and CDS 3' PCR Primer (5'-ATTCTAGAGGCCGAGGCGGCCGACATG-d(T)30N-1N-3', N= A, G, C, or T; N-1=A, G, or C). The SMART Oligonucleotide and the CDS 3' PCR Primer contained Sfi IA and Sfi IB restriction enzyme sites respectively. The first-strand cDNA was used as a template to amplify the double-strand cDNA by long-distance PCR (LD-PCR) with 5' PCR primer (5'-AAGCAGTGGTATCAACGCAGAGT-3') and the CDS 3' PCR Primer. The product was purified and digested by Sfi I (IA and IB) restriction enzyme. The purified double-strand cDNA was ligated into the Sfi I-digested lambdaTripIEx2 vector and the recombinant DNA was packaged in vitro. The phages were transducted to bacteria XL1-Blue and then were amplified.

text: XL1-Blue E.coli

text: Liver

text: Andrias davidianus

text: Sfi IA

text: Sfi IB

text: cDNA Library from the liver of Andrias davidianus

text: Phage


status: PUBLIC

characteristic: External Id

text: University of Pisa

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Platanus x hispanica

characteristic: organism

text: leaf

tag: attribute

characteristic: tissue-type

text: SSH cDNA library Pxa

characteristic: title


text: Ocean University of China

text: live

text: Generic

text: Cells were collected at the logarithmic growth phase. Total RNA was extracted by the guanidine thiocyanate-phenol-chloroform extraction method (Chomczynski and Sacchi 1987). Message RNA was further purified according to the manufacturer's instructions of the Oligotex mRNA Extraction Kits (QIAGEN). Total RNA and mRNA were run on agarose gel to investigate the quality. cDNA synthesis was performed using the pBluescript II SK(+) XR cDNA Library Construction Kit (Stratagene Cloning Systems, California, USA). The library was created in Uni-Zap XR vector using oligo (dT)18 primers and directionally inserted into EcoR I-Xho I sites of pBluescript.

characteristic: description

text: Cells at the logarithmic growth phase

characteristic: dev-stage

text: Nannochloropsis oculata

text: unicellular microalgal cDNA Library

text: phagemid

text: pBluescriptII

characteristic: vector


text: CNRS, INSERM, ULP

text: live

text: Generic

text: myeloid

characteristic: cell type

text: Oligo dT cDNA library constructed from mRNA from myeloid cells.

text: Adult

text: Danio rerio

text: SfiI

text: SfiI

text: myeloid specific cDNA library


text: Bhabha Atomic ResearchCentre

text: live

text: Generic

text: ESTs are identified through the Differential Display techniques

text: one hour soaked seed

text: E. coli

characteristic: lab-host

text: Brassica juncea

text: seed

text: Brassica juncea one hour soaked seed

text: TA cloning


text: Harvard Medical School

text: live

text: Generic

text: Non-normalized library, G50/G178 (gastrula/neurula). cDNA inserts cloned into NotI/SalI sites of pCMVSport6 vector (Invitrogen). 5' end of insert at SalI site of vector.

text: gastrula/neurula

text: Saccoglossus kowalevskii

text: NotI

text: SalI

text: Acorn worm gastrula/neurula pCMVSport6 library

text: plasmid


text: Harvard Medical School

text: live

text: Generic

text: Non-normalized library, G125/G613 (gastrula/neurula). cDNA inserts cloned into NotI/SalI sites of pCMVSport6 vector (Invitrogen). 5' end of insert at SalI site of vector.

text: blastula/gastrula

text: Saccoglossus kowalevskii

text: NotI

text: SalI

text: Acorn worm blastula/gastrula pCMVSport6 library

text: plasmid


text: Harvard Medical School

text: live

text: Generic

text: Non-normalized library, G142 (juvenile). cDNA inserts cloned into NotI/EcoRV sites of pCMVSport6 vector (Invitrogen). 5' end of insert at EcoRV site of vector.

text: juvenile

text: Saccoglossus kowalevskii

text: NotI

text: EcoRV

text: Acorn worm juvenile pCMVSport6 library

text: plasmid


text: Harvard Medical School

text: live

text: Generic

text: Normalized library, G709 (neurula). cDNA inserts cloned into NotI/EcoRV sites of pExpress1 vector (Express Genomics). 5' end of insert at EcoRV site of vector.

text: neurula

text: Saccoglossus kowalevskii

text: NotI

text: EcoRV

text: Acorn worm normalized neurula pExpress1 library

text: plasmid


text: Harvard Medical School

text: live

text: Generic

text: Non-normalized library, SFE1/2 (radial). cDNA inserts cloned into NotI/EcoRV sites of Express1 vector (Express Genomics). 5' end of insert is at EcoRV site of vector.

text: radial

text: Solaster stimpsoni

text: NotI

text: EcoRv

text: Starfish radial pExpress1 library

text: plasmid


text: Shandong Academy of Agricultural Science

text: live

text: Generic

characteristic: cultivar

text: The peanut seeds from DAP (days after pegging) 20 to 60 were collected, and placed into liquid N2 immediately and stored in -80 C freezer. Total RNA was extracted from seeds by RNAgent kit (Promega). mRNA was isolated and purified from total RNA (Promega). Directional cDNA synthesis (using EcoRI, XhoI sites adapters) and library construction followed the protocol of Stratagene's pBluescript II cDNA library construction kit.

text: DAP 20 to 60

text: seeds

characteristic: organ

text: Arachis hypogaea

text: EcoRI

text: XhoI

text: seeds

text: Peanut (Luhua14) seeds full length cDNA Library

text: Plasmid

text: pBluescript II SK


characteristic: External Id

text: University of Ljubljana Faculty of Medicine

text: live

text: Generic

text: Hortaea werneckii

characteristic: strain

text: Hortaea werneckii MZKI-B736


text: The Institute of Physical and Chemical Research (RIKEN)

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: Apis mellifera

text: head

text: RIKEN full-length enriched honey bee cDNA library, head


text: Instituto de Investigaciones Marinas (IIm), CSIC

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Sparus aurata

text: Brain

text: Nodavirus infected sea bream SSH library


text: University of Oklahoma

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Adult Danio rerio 10am (morning) eye/brain cDNA library. Constructed by Yuhong Tang and Han Wang at the Zebrafish Genetics and Genomics Laboratory and sequenced at the Advanced Center of Genomics Research at University of Oklahoma.

text: adult(3 month)

text: eye/brain

text: Danio rerio

text: EcoRI

text: XhoI

text: mixed

characteristic: sex

text: AB

text: phagemid

text: pBlueScriptSK(-) (Stratagene)


text: University of Oklahoma

text: live

text: Generic

text: Adult Danio rerio 12am (night) eye/brain cDNA library. Constructed by Yuhong Tang and Han Wang at the Zebrafish Genetics and Genomics Laboratory and sequenced at the Advanced Center of Genomics Research at University of Oklahoma.

text: adult(3 month)

text: eye/brain

text: Danio rerio

characteristic: organism

text: EcoRI

text: XhoI

text: mixed

text: AB

characteristic: title

text: phagemid

text: pBlueScriptSK(-) (Stratagene)


text: Heilongjiang University

text: live

text: Generic

text: The ESTs were generated from subtractive library with the Suppression Subtractive Hybridization (SSH) between taxol biosynthesis and non-synthesis stage mycelium total RNA of Nodulisporium sylviformeis

characteristic: description

text: Nodulisporium sylviforme

text: Nodulisporium sylviforme subtracted cDNA library

text: Plasmid


text: BC Cancer Agency

text: live

text: Generic

text: 6 months old fetus

characteristic: dev-stage

text: ElectroMAX DH10B T1 Phage-Resistant Cells

text: Placenta

text: Bos taurus

text: SalI (5' end of cDNA)

text: NotI (3' end of cDNA)

text: male

text: Hereford

text: Placenta

characteristic: tissue-type

text: pCMV SPORT 6.0

characteristic: vector


text: Centro de Investigacion y de Estudios Avanzados del IPN

text: live

text: Generic

text: Pinto Villa

characteristic: cultivar

text: Plants of Phaseolus vulgaris cv. Pinto Villa were grown under greenhouse conditions for 30 days after emerging. Relative water content in soil (sRWC) and in leaf (lRWC) was registered as a measure of water status by using the next formula (Bernacchia, Salamini and Bartels, 1996): RWC (%) = [(Fresh wt - Dry wt) / (Turgid wt - Dry wt)] x 100. It was considered lRWC between 50-70%% as intermediate drought stress. Water stress was developed by withholding irrigation from 3 to 8 days representing various dehydration levels. Suppression subtractive hybridization (SSH) was performed by using the PCR-select cDNA subtraction kit (Clontech, USA) according to manufacturer's instructions. The contrasting samples were selected according to RWC data. The driver, or experimental sample, contained mRNA isolated from leaves under intermediate drought conditions. The tester sample, or control sample, contained mRNA isolated from leaves of plants under control conditions.

text: 30 day old plants

text: E. coli TOP 10F

characteristic: lab-host

text: leaves

text: Phaseolus vulgaris

text: RsaI

text: RsaI

text: Phaseolus vulgaris leaves under intermediate drought stress

text: pDrive


characteristic: External Id

text: Hokkaido University

text: live

text: Generic

text: Larix kaempferi

text: xylem

text: Larix kaempferi xylem


text: Missouri State University

text: live

text: Generic

text: Cabernet Sauvignon

text: Powdery mildew-infected Vitis vinifera 'Cabernet sauvignon' leaves were harvested at 0, 4, 8, 12, 24, and 48 h post-inoculation as tissue source for SSH library. Total RNA was extracted individually for each time point. Equimolar amounts of RNA for the 0, 4, and 8 h (early-infection stage) samples and for the 12, 24, and 48 h (late-infection stage) samples were pooled and reverse transcribed to cDNA using BD SMART PCR cDNA synthesis Kit (Clontech, Paolo Alto, California). SSH library was constructed with PCR select cDNA Subtraction Kit (Clontech) using early- and late-infection stage cDNA as tester and driver, respectively. PCR-amplified inserts were sequenced with T7 or SP6 vector-specific primers.

text: young leaf

text: Vitis vinifera

text: hermaphrodite

text: leaf blade

text: Early Infection Stage Subtractive Library

text: Plasmid


text: Missouri State University

text: live

text: Generic

text: Cabernet Sauvignon

text: Powdery mildew-infected Vitis vinifera 'Cabernet sauvignon' leaves were harvested at 0, 4, 8, 12, 24, and 48 h post-inoculation as tissue source for SSH library. Total RNA was extracted individually for each time point. Equimolar amounts of RNA for the 0, 4, and 8 h (early-infection stage) samples and for the 12, 24, and 48 h (late-infection stage) samples were pooled and reverse transcribed to cDNA using BD SMART PCR cDNA synthesis Kit (Clontech, Paolo Alto, California). SSH library was constructed with PCR select cDNA Subtraction Kit (Clontech) using late- and early-infection stage cDNA as tester and driver, respectively. PCR-amplified inserts were sequenced with T7 or SP6 vector-specific primers.

text: young leaf

text: Vitis vinifera

text: hermaphrodite

text: leaf blade

text: Late Infection Stage Subtractive Library

text: plasmid


text: Missouri State University

text: live

text: Generic

text: Cabernet Sauvignon

text: Powdery mildew-infected and healthy leaves of Vitis vinifera 'Cabernet sauvignon' were harvested at 0, 4, 8, 12, 24, and 48 h after inoculation (mildew-infected) or 0, 4, 8, 12, 24, and 48 h after mock-inoculation (healthy control) inoculation as tissue source for SSH library. Total RNA was extracted individually for each time point of each infected and mock-inoculated sample. Equimolar amounts of RNA were pooled and reverse transcribed to cDNA using BD SMART PCR cDNA Synthesis Kit (Clontech, Paolo Alto, California). SSH library was constructed with PCR Select cDNA Subtraction Kit (Clontech), using infected tissue-derived cDNA as tester and mock-inoculated tissue-derived cDNA as driver. PCR-amplified inserts were sequenced with T7 or SP6 vector-specific primers.

text: young leaf

text: Vitis vinifera

text: hermaphrodite

text: leaf blade

text: All Infection Stages Subtractive Library

text: plasmid


text: Boyce Thompson Institute for Plant Research

text: live

text: Generic

text: adult

text: Myzus persicae

text: female

text: Freeville 001

characteristic: strain

text: whole insect

text: Myzus persicae, line F001, whole female aphid library

text: plasmid

text: pDNR-LIB


characteristic: INSDC center name

text: live

text: Generic

text: winged adult

text: Myzus persicae

text: male

text: Freeville 001

text: whole insect

text: Myzus persicae, line F001, whole male aphid library

text: plasmid

text: pDNR-LIB


characteristic: INSDC first public

text: live

text: Generic

text: mixed instars

text: Myzus persicae

text: parthenogenetic female

text: Freeville 001

text: whole insect

text: Myzus persicae, line F001, PLRV infected, whole aphid library

text: plasmid

text: pDNR-LIB


characteristic: INSDC last update

text: live

text: Generic

text: adult

text: Myzus persicae

text: female

text: Geneva 006

text: whole insect

text: Myzus persicae, line G006, whole female aphid library

text: plasmid

text: pDNR-LIB


text: live

characteristic: INSDC status

text: Generic

text: adult

text: Myzus persicae

text: parthenogenetic female

text: tobacco adapted lineage (red)

text: salivary gland

text: Myzus persicae, tobacco lineage, aphid salivary gland library

text: plasmid

text: pDNR-LIB


text: Scottish Crop Research Institute

text: live

text: Generic

characteristic: NCBI submission model

text: Glen Ample

text: 0 - 1200 Chilling Units

text: Rubus idaeus

text: buds

text: Endodormant buds from Rubus idaeus L. cv Glen Ample

text: plasmid

text: pBluescript


text: Scottish Crop Research Institute

text: live

text: Generic

characteristic: NCBI submission package

text: Glen Ample

text: 1200 - 2500 Chilling Units

text: Rubus idaeus

text: buds

text: Paradormant buds from Rubus idaeus L. cv Glen Ample

text: plasmid


text: Northeast Forestry University

text: live

text: Generic

text: Betula platyphylla

text: flower

text: SSH library of Betula platyphylla Suk female inflorescence


text: Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa

text: live

text: Generic

text: adult

text: midgut

characteristic: organ

text: Anopheles albimanus

text: female

characteristic: sex

text: Anopheles albimanus midgut


text: National Cancer Institute / NIH

text: live

text: Generic

text: cDNA was primed using oligo-dT primer: GACTAGTTCTAGATCGCGAGCGGCCGCC(T)25 and cloned into the SmaI/NotI sites of pCS111. Size-selection >1.25kb resulted in an average insert size of 1.7 kb. This primary library was constructed by Express Genomics (Frederick, MD). Note: this is a (http://xgc.nci.nih.gov/) Xenopus Gene Collection library.

text: DH10B TonA

text: pancreas

text: Xenopus laevis

characteristic: organism

text: SmaI

text: NotI

text: whole pancreas, 3 pooled

characteristic: title

text: plasmid


text: National Cancer Institute / NIH

text: live

text: Generic

text: cDNA was primed using oligo-dT primer: GACTAGTTCTAGATCGCGAGCGGCCGCC(T)25 and cloned into the SmaI/NotI sites of pCS111. Size-selection >1.25kb resulted in an average insert size of 1.65 kb. This primary library was constructed by Express Genomics (Frederick, MD). Note: this is a (http://xgc.nci.nih.gov/) Xenopus Gene Collection library.

characteristic: description

text: DH10B TonA

text: bone

text: Xenopus laevis

text: SmaI

text: NotI

text: limb bones (zeugopods and stylopods), pooled from 3 individuals

text: plasmid


text: National Cancer Institute / NIH

text: live

text: Generic

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer 5'-GACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3', double-stranded cDNA was cloned into the NotI and EcoRV sites of pExpress-1 (EcoRV site destroyed upon ligation). Library was size-selected for an average insert size of 1.9 kb. Primary library, non-amplified. Use M13(-21) to generate 3' end reads and M13R to generate 5' end reads. Library was constructed by Open Biosystems (Huntsville, AL). Note: this is a (http://zgc.nci.nih.gov/) Zebrafish Gene Collection library.

text: DH10B TonA

text: embryo

text: Danio rerio

text: EcoRV

text: NotI

text: ZEM-2S embryo cell line (ATCC cat # CRL2147), 1000 cell blastula at 5 hrs postfertilization

characteristic: tissue-type

text: plasmid


text: National Cancer Institute / NIH

text: live

text: Generic

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer 5'-GACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3', double-stranded cDNA was cloned into the NotI (3') and EcoRV (5') sites of pExpress-1 (EcoRV site destroyed upon ligation). Library has an average insert size of 1.7 kb. Primary library, non-amplified. Use M13(-21) to generate 3' end reads and M13R to generate 5' end reads. Library was constructed by Open Biosystems (Huntsville, AL). Note: this is a (http://zgc.nci.nih.gov/) Zebrafish Gene Collection library.

text: DH10B TonA

text: testis

text: Danio rerio

text: EcoRV

text: NotI

text: whole testes, pooled

text: plasmid

characteristic: vector


text: Capital Normal University

text: live

text: Generic

text: Physcomitrium patens

text: P. patens Differentially Express Library


text: Consejo Superior de Investigaciones Cientificas

text: live

text: Generic

text: Gloire de Montpellier

characteristic: cultivar

text: mRNA was extracted from plants growing in hydroponic conditions under different iron stress conditions

text: 1, 7 or 14 days after treatments started

characteristic: dev-stage

text: root

text: Vitis riparia

text: cDNA-AFLP Vitis iron stress

text: plasmid

text: pGEM-T


characteristic: External Id

text: Institut National de la Recherche Agronomique

text: live

text: Generic

text: Cabernet Sauvignon

text: mRNA was extracted from plants growing in vitro conditions under different iron and bicarbonate stress treatments

text: root

text: Vitis vinifera

text: cDNA-AFLP Vitis abiotic stress

text: plasmid

text: pGEM-T


text: Florida A & M University

text: live

text: Generic

text: Used RT-PCR to isolate tissue specific mRNA from grape berry.

text: Fruit

text: Vitis rotundifolia

text: Berry tissue

text: Grape berry tissue express library


text: University of Oklahoma

text: live

text: Generic

text: Sperm cell

characteristic: cell type

text: cDNA was prepared from isolated Plumbago zeylanica sperm cells and amplified by PCR with the help of SMART PCR cDNA Synthesis kit (Clontech).Suppression subtractive hybridizations between Sua and Svn sperm cells were then performed using the PCR-Select cDNA Subtraction kit (Clontech).The purified subtractive Sua and Svn cDNAs were cloned into pCR2.1-TOPO vector (Invitrogen) and transformed into Escherichia coli XL10-Gold cells (Stratagene) to obtain subtracted Sua and Svn cDNA libraries, respectively.

text: mature pollen of Plumbago zeylanica

text: E.coli XL10-Gold

characteristic: lab-host

text: Plumbago zeylanica

text: Subtracted cDNA library of Plumbago zeylanica Sua sperm cell

text: Plasmid


text: University of Oklahoma

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: Sperm cell

text: cDNA was prepared from isolated Plumbago zeylanica sperm cells and amplified by PCR with the help of SMART PCR cDNA Synthesis kit (Clontech). Suppression subtractive hybridizations between Sua and Svn sperm cells were then performed using the PCR-Select cDNA Subtraction kit (Clontech).The purified subtractive Sua and Svn cDNAs were cloned into pCR2.1-TOPO vector (Invitrogen) and transformed into Escherichia coli XL10-Gold cells (Stratagene) to obtain subtracted Sua and Svn cDNA libraries, respectively.

text: mature pollen of Plumbago zeylanica

text: E.coli XL10-Gold

text: Plumbago zeylanica

text: Subtracted cDNA library of Plumbago zeylanica Svn sperm cell

text: Plasmid


text: Universitaet Hamburg, Biozentrum Klein Flottbek

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Hybrid UH301xUH005

text: SMART cDNA synthesis and PCR-Select cDNA subtraction (Clontech) of Hybrid cDNA with parental line cDNA, PCR products T/A cloned

text: 6 days after pollination

text: E. coli, XL1 Blue

text: Zea mays

text: embryo

text: pGEM T-Easy, Promega


text: INRA - SCRIBE

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Clone distribution : AGENAE Resource centre. Francois PIUMI, Francois.Piumi@jouy.inra.fr, INRA, CEA Radiobiologie et Etude du genome (LREG), Domaine de Vilvert, 78352, Jouy-en-Josas cedex, FRANCE, +33 (0) 1.34.65.28.02, +33 (0) 1.34.65.22.73

text: from embryos to adults

text: Oncorhynchus mykiss

text: AGENAE Rainbow trout multi-tissues library (tcce)

text: plasmid

text: pT7T3D pac - pCMVSport


text: Shandong University,School of Life Sciences

text: live

text: Generic

text: Molting stage

text: E.coli DH5alpha

text: Whole body

characteristic: organ

text: Helicoverpa armigera

text: Whole body

text: Cotton boll worm Suppression Subtractive Hybridization (SSH) Library

text: pGEMT Easy


text: Chulalongkorn University

text: live

text: Generic

text: The cDNA library was generated as part of Penaeus monodon EST project. Total RNA was isolated from hemocyte of V. harveyi infected Penaeus monodon. The mRNA was further purified, reverse transcribed and cloned to Lambda ZAPII.

text: Penaeus monodon

characteristic: organism

text: Not I

text: Sal I

text: Hemocyte

text: Hemocyte V. harveyi infected library

text: Phage

text: Lambda ZAP II


text: The Hebrew University

text: live

text: Generic

text: Azospirillum brasilense

text: Azospirillum brasilense cDNA-AFLP fragment

characteristic: title


text: National Center of Excellence in Molecular Biology

text: live

text: Generic

text: ESTs were obtained through differential display (Liang et al, 1992.) that appeared under water deficit conditions. Identified ESTs were TA cloned in PCR 2.1 vector and sequenced.

characteristic: description

text: 10 days of stress

text: LEAF

text: Gossypium arboreum

text: hermaphrodite

characteristic: sex

text: Total leaf tissue

text: Differential display of COTTON GENOTYPE, leaf

text: Plasmid


text: Huazhong Agricultural University

text: live

text: Generic

text: fertile and sterile anthers were harvested at PMC stage, and then snap-frozen in liquid nitrogen. The subtracted library was constructed as described in the PCR-Select cDNA Subtraction Kit.

text: pollen mother cell (PMC)

text: Brassica napus

text: anther

characteristic: tissue-type

text: suppression subtractive hybridization library of dominant genic male sterile (DGMS) Brassica napus

text: Plasmid

text: p-GEMT

characteristic: vector


text: University Medical Center Goettingen

text: live

text: Generic

text: Homo sapiens

text: brain

text: Homo sapiens brain (Tzvetkov)


text: University Medical Center Goettingen

text: live

text: Generic

text: Homo sapiens

text: colon

text: Homo sapiens colon (Tzvetkov)


text: University Medical Center Goettingen

text: live

text: Generic

text: Homo sapiens

text: small intestine

text: Homo sapiens small intestine (Tzvetkov)


text: European Molecular Biology Laboratory

text: live

text: Generic

text: stages 18, 24, 32, 36

characteristic: dev-stage

text: Oryzias latipes

text: Cab

characteristic: strain

text: whole embryo

text: Oryzias latipes whole embryo Cab stages 18, 24, 32, 36


characteristic: External Id

text: Hunan Agricultural University

text: live

text: Generic

text: liver

text: Sus scrofa

text: liver

text: swine liver ETEC F4R


text: Institute of Zoology, Chinese Academy of Sciences

text: live

text: Generic

text: adult

characteristic: lab-host

text: Gobiocypris rarus

text: rare minnow liver cDNA Library

text: phagemid

text: pBluescriptII


text: Institute of Plant Physiology of Shanghai Institutes for Biological Sciences, The Chinese Acadamy of Sciences

characteristic: INSDC center name

text: live

text: Generic

text: 5th instar larvae

text: Midgut

text: Helicoverpa armigera

text: Helicoverpa armigera midgut Lambda Zap Express Library

text: Phage


text: Warwick University

characteristic: INSDC first public

text: live

text: Generic

characteristic: cultivar

text: Brassica oleracea var. alboglabra

text: etiolated seedling


text: University of Nebraska-Lincoln

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: E. coli

text: Glycine max

text: Leaf tissue

text: Soybean severe stunt virus-infected soybean cDNA library (slv1c)

text: pBS SKII


text: National Research Council of Canada

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Sequences have been trimmed to remove vector and low quality regions using LUCY sequence cleanup software (www.tigr.org).

text: Brassica napus

text: Brassica napus - BNHA

text: plasmid


text: Plant Biotechnology Institute, National Research Council Canada

text: live

text: Generic

text: Brassica napus

characteristic: organism

text: Brassica seed development drought not normalized FTYFDB

text: plasmid

text: pGemT-Easy


text: live

text: Generic

text: Brassica napus

text: Sfi IA

text: Sfi IB

text: 3 week old whole seedling, including roots, no cotyledons

text: Experiment 24 test plate lig 3 EX24L3

characteristic: title

text: plasmid

text: pHSX-C


text: National Research Council of Canada

text: live

text: Generic

characteristic: description

text: Brassica napus

text: Brassica napus - 9RDBNGA

text: plasmid


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: plasmid


text: live

text: Generic

text: Brassica napus

text: Brassica seed development not normalized FTYFDA

text: plasmid

text: pGemT-Easy


text: National Research Council of Canada

text: live

text: Generic

text: Brassica carinata

text: EcoRI

text: EcoRI

text: Brassica carinata - Xiang2

text: plasmid


text: live

text: Generic

text: Brassica napus

text: 6th true leaf

text: Brassica napus EX2PLCL3

text: plasmid

text: pGemT-Easy


text: live

text: Generic

text: Brassica napus

text: Brassica napus Ex 20-Lib9

text: plasmid

text: pGemT-Easy


text: live

text: Generic

text: Brassica napus

text: Brassica FNYSU

text: plasmid

text: pGemT-Easy


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Brassica napus seeds BNYS6DCT

text: plasmid


text: live

text: Generic

text: Brassica napus

text: 4th and 6th true leaves

text: Brassica napus Expt 2 and 4 rerun materials EX24ATES

text: plasmid


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Brassica napus seeds BNYS2DCT

text: plasmid


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Sfi I A

text: Sfi I B

text: Brassica napus 34ETGS48

text: plasmid


text: live

text: Generic

text: Brassica napus

text: 4th true leaf

text: Brassica napus EX4PLCL1

text: plasmid

text: pGemT-Easy


text: live

text: Generic

text: Brassica napus

text: Brassica seed development normalized FNYFDD

text: plasmid

text: pGemT-Easy


text: live

text: Generic

text: Brassica napus

text: Brassica seed development normalized FNYFDA

text: plasmid

text: pGemT-Easy


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Brassica napus AC Excell

text: plasmid


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Brassica napus DH12075

text: plasmid


text: live

text: Generic

text: Brassica napus

text: Brassica seed development drought normalized FNYFDB

text: plasmid

text: pGemT-Easy


text: National Research Council of Canada

text: live

text: Generic

text: Brassica rapa

text: Sfi IA

text: Sfi IB

text: Brassica rapa 37RDBRH

text: plasmid


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Brassica napus

text: plasmid


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Sfi I A

text: Sfi I B

text: Brassica napus seed library

text: plasmid


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: sfi IA

text: sfi IB

text: Brassica napus BNZA

text: plasmid


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Sfi I A

text: Sfi I B

text: Brassica napus 59ACAB6

text: plasmid


text: National Research Council of Canada

text: live

text: Generic

text: Brassica oleracea

text: Sfi I A

text: Sfi I B

text: O. Alba 49RDOAT

text: plasmid


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Sfi I A

text: Sfi I B

text: Brassica napus 35ETGS60

text: plasmid


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Brassica napus seeds BNBS8DCT

text: plasmid


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Brassica napus BNZB

text: plasmid


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Brassica napus seeds BNYS8DCT

text: plasmid


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Sfi IA

text: Sfi IB

text: Brassica napus 5ETGS9h

text: plasmid


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Brassica napus seed coat BNSCS4CT

text: plasmid


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Brassica napus seeds BNYS4DCT

text: plasmid


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Sfi IA

text: Sfi IB

text: Brassica napus 31ETGS12

text: plasmid


text: live

text: Generic

text: Brassica napus

text: 4th true leaf

text: Brassica napus EX4PLCL3

text: plasmid

text: pGemT-Easy


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: plasmid


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Sfi IA

text: Sfi IB

text: Brassica napus 61RDBNCM

text: plasmid


text: live

text: Generic

text: Brassica napus

text: Brassica seed development normalized FNYFDC

text: plasmid

text: pGemT-Easy


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Brassica napus seed coat BNSCS5CT

text: plasmid


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: BRASSICA NAPUS SEEDS BNDH8DCT

text: plasmid


text: live

text: Generic

text: Brassica napus

text: Brassica napus EX20LIB6

text: plasmid

text: pGemT-Easy


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Sfi IA

text: Sfi IB

text: Brassica napus 6ETGS6h

text: plasmid


text: live

text: Generic

text: Brassica napus

text: Sfi IA

text: Sfi IB

text: 3 week old whole seedling, including roots, excluding cotyle

text: Brassica napus EX24LIG3

text: plasmid

text: pHSX-C


text: live

text: Generic

text: Brassica napus

text: Sfi IA

text: Sfi IB

text: Experiment 24 test plate lig 2 EX24L2

text: plasmid

text: pHSX-C


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Brassica napus seed coat BNSCS2CT

text: plasmid


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Sfi IB

text: Sfi IC

text: full length germinating seed library 66ETFLGS

text: plasmid

text: pHSX-C


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: BRASSICA NAPUS SEEDS BNDH6DCT

text: plasmid


text: National Research Council of Canada

text: live

text: Generic

text: Brassica oleracea

text: Sfi I A

text: Sfi I B

text: O. Alba 46RDOAG

text: plasmid


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Sfi IB

text: Sfi IC

text: germinating seed library 67ETGS

text: plasmid

text: pHSX-C


text: live

text: Generic

text: Brassica napus

text: Brassica seed development drought normalized FTYFDC

text: plasmid

text: pGemT-Easy


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Brassica napus seeds BNBS4DCT

text: plasmid


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Brassica napus seeds BNBS6DCT

text: plasmid


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Sfi I A

text: Sfi I B

text: Brassica napus 33ETGS36

text: plasmid


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: EcoR I

text: Xho I

text: Brassica napus mature seed Normalized library at CoT 5

text: plasmid


text: live

text: Generic

text: Brassica napus

text: Brassica napus EX20LIB5

text: plasmid

text: pGemT-Easy


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Sfi IA

text: Sfi IB

text: Brassica napus BNQA

text: plasmid


text: National Research Council of Canada

text: live

text: Generic

text: Brassica rapa

text: Sfi IA

text: Sfi IB

text: Brassica rapa 36RDBRG

text: plasmid


text: live

text: Generic

text: Brassica napus

text: 6th true leaf

text: Brassica napus EX2PLCL1

text: plasmid

text: pGemT-Easy


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Brassica napus seed coat BNSCS3CT

text: plasmid


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Sfi IA

text: Sfi IB

text: Brassica napus 32ETGS24

text: plasmid


text: live

text: Generic

text: Brassica napus

text: Brassica napus Ex 20-Lib7

text: plasmid

text: pGemT-Easy


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Brassica napus - BNZDA

text: plasmid


text: live

text: Generic

text: Brassica napus

text: Brassica napus Ex 20-Lib8

text: plasmid

text: pGemT-Easy


text: live

text: Generic

text: Brassica napus

text: Sfi IA

text: Sfi IB

text: Brassica napus EX24LIG2

text: plasmid

text: pHSX-C


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Brassica napus seeds BNBS2DCT

text: plasmid


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Sfi I A

text: Sfi I B

text: Germinating seeds 2h

text: plasmid


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Sfi IA

text: Sfi IB

text: Brassica napus 15ACDHYS

text: plasmid


text: National Research Council of Canada

text: live

text: Generic

text: Brassica carinata

text: SfiIA

text: SfiIB

text: Brassica carinata - 7RDBCH

text: plasmid


text: live

text: Generic

text: Brassica napus

text: Brassica FNYSD

text: plasmid

text: pGemT-Easy


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: plasmid


text: Laboratorio Nacional de Genomica para la Biodiversidad, Cinvestav Campus Guanajuato

text: live

text: Generic

text: cDNA library obtained using a pool of polyA+ RNA extracted from T. atroviride grown in submerged culture in minimal medium using Rhizoctonia solani cell walls as sole carbon source. Mycelia were collected at 6, 12 and 24h.

text: Trichoderma atroviride


text: live

text: Generic

text: cDNA library obtained using a pool of polyA+ RNA extracted from T. atroviride grown on Potato Dextrose Agar that was exposed to a 5 minutes pulse of blue light, mycelia was collected 5 and 30 min after exposure to light.

text: Trichoderma atroviride


text: live

text: Generic

text: Constructed using the PCR-SelectTM cDNA Subtraction System (Clontech) using as tester Trichoderma polyA+ RNA purified after exposure of the fungus to a 5 minutes pulse of blue light and collected 30 minutes after exposure to light and as driver polyA+ RNA from a control kept in the dark.

text: Trichoderma atroviride

text: pCR II-Topo


text: live

text: Generic

text: Constructed using the PCR-SelectTM cDNA Subtraction System (Clontech) using as tester Trichoderma polyA+ RNA purified from dark grown mycelia and as driver polyA+ RNA of blue light induced mycelia collected 30 minutes after exposure to light.

text: Trichoderma atroviride

text: pCR II-Topo


text: live

text: Generic

text: Constructed using the PCR-SelectTM cDNA Subtraction System (Clontech) using as tester Trichoderma polyA+ RNA purified after exposure of the fungus to a 5 minutes pulse of blue light and collected 15 minutes after exposure to light and as driver polyA+ RNA from a control kept in the dark.

text: Trichoderma atroviride

text: pCR II-Topo


text: live

text: Generic

text: Constructed using the PCR-SelectTM cDNA Subtraction System (Clontech) using as tester Trichoderma polyA+ RNA purified from dark grown mycelia and as driver polyA+ RNA of blue light induced mycelia collected 5 minutes after exposure to light.

text: Trichoderma atroviride

text: pCR II-Topo


text: Michigan State University Department of Horticulture/Washington Tree Fruit Research Commission/Michigan Agricultural Experiment Station

text: live

text: Generic

text: Montmorency

text: Young-green and pre-ripe

text: Fruit

characteristic: organ

text: Prunus cerasus

text: Pericarp

text: YG/PR


text: Key Laboratory of Forest Plant Ecology (KLP), Ministry of Education

text: live

text: Generic

text: The greenhouse grown corktree seedlings from DAB (days after burgeoning) 60 to 65 were rapidly removed from the pumice by washing and drying on absorbent papers before placing intact in a climatic chamber(day cycle). Leaves were separated then frozen in liquid N2 after 2 h of dehydration. Total RNA was extracted by Trizol Reagent (Invitrogen). mRNA was isolateded and purified from total RNA (Qiagen). Library construction followed the user manual of Clotech PCR-Select cDNA subtraction Kit.

text: DAB (days after burgeoning) 60 to 65

text: TOP10 cells

text: Phellodendron amurense

text: NcoI

text: NotI

text: Leaves

characteristic: tissue-type

text: Amur corktree subtraction cDNA library under water stress

text: Plasmid

text: pGEM-T


text: South China Institute of Endangered Animals

text: live

text: Generic

text: brain

text: Rhinolophus ferrumequinum

text: brain

text: hibernating bat brain SSH


text: Centro de Investigacion en Alimentacion y Desarrollo AC

text: live

text: Generic

text: Penaeus vannamei

text: white spot syndrome virus infected hemocyte library


text: National Institute of Agrobiological Sciences

text: live

text: Generic

text: adult

text: Sus scrofa

text: adrenal gland

text: full-length enriched swine cDNA library, adult adrenal gland


text: live

text: Generic

text: immature dendritic cell

characteristic: cell type

text: adult

text: Sus scrofa

text: full-length enriched swine cDNA library, adult immature dendritic cell


text: live

text: Generic

text: adult

characteristic: dev-stage

text: Sus scrofa

text: skin

text: full-length enriched swine cDNA library, adult skin


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAM05 from Apac, Uganda originated from a patient sufferring from mild malaria. Erythrocytes were separated from other blood constituents and cultured until parasites matured into trophozoites. Trophozoites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

characteristic: isolate

text: Homo sapiens

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UAM05 Pf var gene cDNA

text: pcrII-TOPO

characteristic: vector


characteristic: External Id

text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAM08 from Apac, Uganda originated from a patient sufferring from mild malaria. Erythrocytes were separated from other blood constituents and cultured until parasites matured into trophozoites. Trophozoites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Homo sapiens

characteristic: lab-host

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UAM08 Pf var gene cDNA

text: pcrII-TOPO


text: University of Bern

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAM09 from Apac, Uganda originated from a patient sufferring from mild malaria. Erythrocytes were separated from other blood constituents and cultured until parasites matured into trophozoites. Trophozoites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Homo sapiens

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UAM09 Pf var gene cDNA

text: pcrII-TOPO


text: University of Bern

characteristic: INSDC last update

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAM11 from Apac, Uganda originated from a patient sufferring from mild malaria. Erythrocytes were separated from other blood constituents and cultured until parasites matured into trophozoites. Trophozoites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Homo sapiens

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UAM11 Pf var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

characteristic: INSDC status

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAM12 from Apac, Uganda originated from a patient sufferring from mild malaria. Erythrocytes were separated from other blood constituents and cultured until parasites matured into trophozoites. Trophozoites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Homo sapiens

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UAM12 Pf var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

characteristic: NCBI submission model

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAM13 from Apac, Uganda originated from a patient sufferring from mild malaria. Erythrocytes were separated from other blood constituents and cultured until parasites matured into trophozoites. Trophozoites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Homo sapiens

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UAM13 Pf var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

characteristic: NCBI submission package

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAM14 from Apac, Uganda originated from a patient sufferring from mild malaria. Erythrocytes were separated from other blood constituents and cultured until parasites matured into trophozoites. Trophozoites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Homo sapiens

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UAM14 Pf var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAM15 from Apac, Uganda originated from a patient sufferring from mild malaria. Erythrocytes were separated from other blood constituents and cultured until parasites matured into trophozoites. Trophozoites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Homo sapiens

text: Plasmodium falciparum

characteristic: organism

text: Blood

text: Ugandan isolate UAM15 Pf var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAM16 from Apac, Uganda originated from a patient sufferring from mild malaria. Erythrocytes were separated from other blood constituents and cultured until parasites matured into trophozoites. Trophozoites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Homo sapiens

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UAM16 Pf var gene cDNA

characteristic: title

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAM17 from Apac, Uganda originated from a patient sufferring from mild malaria. Erythrocytes were separated from other blood constituents and cultured until parasites matured into trophozoites. Trophozoites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

characteristic: description

text: Trophozoite

text: Homo sapiens

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UAM17 Pf var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAM18 from Apac, Uganda originated from a patient sufferring from mild malaria. Erythrocytes were separated from other blood constituents and cultured until parasites matured into trophozoites. Trophozoites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Homo sapiens

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UAM18 Pf var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAM20 from Apac, Uganda originated from a patient sufferring from mild malaria. Erythrocytes were separated from other blood constituents and cultured until parasites matured into trophozoites. Trophozoites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Homo sapiens

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UAM20 Pf var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAM21 from Apac, Uganda originated from a patient sufferring from mild malaria. Erythrocytes were separated from other blood constituents and cultured until parasites matured into trophozoites. Trophozoites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Homo sapiens

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UAM21 Pf var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAM22 from Apac, Uganda originated from a patient sufferring from mild malaria. Erythrocytes were separated from other blood constituents and cultured until parasites matured into trophozoites. Trophozoites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Homo sapiens

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UAM22 Pf var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAM23 from Apac, Uganda originated from a patient sufferring from mild malaria. Erythrocytes were separated from other blood constituents and cultured until parasites matured into trophozoites. Trophozoites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Homo sapiens

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UAM23 Pf var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAM25 from Apac, Uganda originated from a patient sufferring from mild malaria. Erythrocytes were separated from other blood constituents and cultured until parasites matured into trophozoites. Trophozoites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Homo sapiens

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UAM25 Pf var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAM26 from Apac, Uganda originated from a patient sufferring from mild malaria. Erythrocytes were separated from other blood constituents and cultured until parasites matured into trophozoites. Trophozoites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Homo sapiens

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UAM26 Pf var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAM27 from Apac, Uganda originated from a patient sufferring from mild malaria. Erythrocytes were separated from other blood constituents and cultured until parasites matured into trophozoites. Trophozoites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Homo sapiens

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UAM27 Pf var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAM28 from Apac, Uganda originated from a patient sufferring from mild malaria. Erythrocytes were separated from other blood constituents and cultured until parasites matured into trophozoites. Trophozoites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Homo sapiens

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UAM28 Pf var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAM29 from Apac, Uganda originated from a patient sufferring from mild malaria. Erythrocytes were separated from other blood constituents and cultured until parasites matured into trophozoites. Trophozoites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Homo sapiens

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UAM29 Pf var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAM31 from Apac, Uganda originated from a patient sufferring from mild malaria. Erythrocytes were separated from other blood constituents and cultured until parasites matured into trophozoites. Trophozoites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Homo sapiens

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UAM31 Pf var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAM32 from Apac, Uganda originated from a patient sufferring from mild malaria. Erythrocytes were separated from other blood constituents and cultured until parasites matured into trophozoites. Trophozoites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Homo sapiens

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UAM32 Pf var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAM33 from Apac, Uganda originated from a patient sufferring from mild malaria. Erythrocytes were separated from other blood constituents and cultured until parasites matured into trophozoites. Trophozoites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Homo sapiens

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UAM33 Pf var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAM34 from Apac, Uganda originated from a patient sufferring from mild malaria. Erythrocytes were separated from other blood constituents and cultured until parasites matured into trophozoites. Trophozoites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Homo sapiens

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UAM34 Pf var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAM35 from Apac, Uganda originated from a patient sufferring from mild malaria. Erythrocytes were separated from other blood constituents and cultured until parasites matured into trophozoites. Trophozoites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Homo sapiens

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UAM35 Pf var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAM36 from Apac, Uganda originated from a patient sufferring from mild malaria. Erythrocytes were separated from other blood constituents and cultured until parasites matured into trophozoites. Trophozoites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Homo sapiens

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UAM36 Pf var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAM37 from Apac, Uganda originated from a patient sufferring from mild malaria. Erythrocytes were separated from other blood constituents and cultured until parasites matured into trophozoites. Trophozoites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Homo sapiens

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UAM37 Pf var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAM38 from Apac, Uganda originated from a patient sufferring from mild malaria. Erythrocytes were separated from other blood constituents and cultured until parasites matured into trophozoites. Trophozoites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Homo sapiens

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UAM38 Pf var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAM39 from Apac, Uganda originated from a patient sufferring from mild malaria. Erythrocytes were separated from other blood constituents and cultured until parasites matured into trophozoites. Trophozoites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Homo sapiens

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UAM39 Pf var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAM40 from Apac, Uganda originated from a patient sufferring from mild malaria. Erythrocytes were separated from other blood constituents and cultured until parasites matured into trophozoites. Trophozoites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Homo sapiens

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UAM40 Pf var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAM41 from Apac, Uganda originated from a patient sufferring from mild malaria. Erythrocytes were separated from other blood constituents and cultured until parasites matured into trophozoites. Trophozoites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Homo sapiens

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UAM41 Pf var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAM42 from Apac, Uganda originated from a patient sufferring from mild malaria. Erythrocytes were separated from other blood constituents and cultured until parasites matured into trophozoites. Trophozoites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Homo sapiens

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UAM42 Pf var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAM43 from Apac, Uganda originated from a patient sufferring from mild malaria. Erythrocytes were separated from other blood constituents and cultured until parasites matured into trophozoites. Trophozoites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Homo sapiens

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UAM43 Pf var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAM44 from Apac, Uganda originated from a patient sufferring from mild malaria. Erythrocytes were separated from other blood constituents and cultured until parasites matured into trophozoites. Trophozoites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Homo sapiens

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UAM44 Pf var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAM45 from Apac, Uganda originated from a patient sufferring from mild malaria. Erythrocytes were separated from other blood constituents and cultured until parasites matured into trophozoites. Trophozoites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Homo sapiens

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UAM45 Pf var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAM46 from Apac, Uganda originated from a patient sufferring from mild malaria. Erythrocytes were separated from other blood constituents and cultured until parasites matured into trophozoites. Trophozoites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Homo sapiens

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UAM46 Pf var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAM47 from Apac, Uganda originated from a patient sufferring from mild malaria. Erythrocytes were separated from other blood constituents and cultured until parasites matured into trophozoites. Trophozoites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Homo sapiens

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UAM47 Pf var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAM48 from Apac, Uganda originated from a patient sufferring from mild malaria. Erythrocytes were separated from other blood constituents and cultured until parasites matured into trophozoites. Trophozoites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Homo sapiens

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UAM48 Pf var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAM49 from Apac, Uganda originated from a patient sufferring from mild malaria. Erythrocytes were separated from other blood constituents and cultured until parasites matured into trophozoites. Trophozoites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Homo sapiens

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UAM49 Pf var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAM50 from Apac, Uganda originated from a patient sufferring from mild malaria. Erythrocytes were separated from other blood constituents and cultured until parasites matured into trophozoites. Trophozoites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Homo sapiens

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UAM50 Pf var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAM51 from Apac, Uganda originated from a patient sufferring from mild malaria. Erythrocytes were separated from other blood constituents and cultured until parasites matured into trophozoites. Trophozoites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Homo sapiens

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UAM51 Pf var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAM52 from Apac, Uganda originated from a patient sufferring from mild malaria. Erythrocytes were separated from other blood constituents and cultured until parasites matured into trophozoites. Trophozoites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Homo sapiens

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UAM52 Pf var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAM53 from Apac, Uganda originated from a patient sufferring from mild malaria. Erythrocytes were separated from other blood constituents and cultured until parasites matured into trophozoites. Trophozoites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Homo sapiens

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UAM53 Pf var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAS03 from Apac, Uganda originated from a patient sufferring from severe malaria. Erythrocytes were separated from other blood constituents and cultured until parasites matured into trophozoites. Trophozoites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Homo sapiens

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UAS03 Pf var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAS06 from Apac, Uganda originated from a patient sufferring from severe malaria. Erythrocytes were separated from other blood constituents and cultured until parasites matured into trophozoites. Trophozoites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Homo sapiens

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UAS06 Pf var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAS07 from Apac, Uganda originated from a patient sufferring from severe malaria. Erythrocytes were separated from other blood constituents and cultured until parasites matured into trophozoites. Trophozoites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Homo sapiens

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UAS07 Pf var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAS08 from Apac, Uganda originated from a patient sufferring from severe malaria. Erythrocytes were separated from other blood constituents and cultured until parasites matured into trophozoites. Trophozoites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Homo sapiens

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UAS08 Pf var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAS10 from Apac, Uganda originated from a patient sufferring from severe malaria. Erythrocytes were separated from other blood constituents and cultured until parasites matured into trophozoites. Trophozoites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Homo sapiens

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UAS10 Pf var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAS11 from Apac, Uganda originated from a patient sufferring from severe malaria. Erythrocytes were separated from other blood constituents and cultured until parasites matured into trophozoites. Trophozoites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Homo sapiens

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UAS11 Pf var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAS12 from Apac, Uganda originated from a patient sufferring from severe malaria. Erythrocytes were separated from other blood constituents and cultured until parasites matured into trophozoites. Trophozoites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Homo sapiens

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UAS12 Pf var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAS13 from Apac, Uganda originated from a patient sufferring from severe malaria. Erythrocytes were separated from other blood constituents and cultured until parasites matured into trophozoites. Trophozoites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Homo sapiens

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UAS13 Pf var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAS14 from Apac, Uganda originated from a patient sufferring from severe malaria. Erythrocytes were separated from other blood constituents and cultured until parasites matured into trophozoites. Trophozoites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Homo sapiens

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UAS14 Pf var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAS16 from Apac, Uganda originated from a patient sufferring from severe malaria. Erythrocytes were separated from other blood constituents and cultured until parasites matured into trophozoites. Trophozoites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Homo sapiens

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UAS16 Pf var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAS17 from Apac, Uganda originated from a patient sufferring from severe malaria. Erythrocytes were separated from other blood constituents and cultured until parasites matured into trophozoites. Trophozoites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Homo sapiens

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UAS17 Pf var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAS18 from Apac, Uganda originated from a patient sufferring from severe malaria. Erythrocytes were separated from other blood constituents and cultured until parasites matured into trophozoites. Trophozoites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Homo sapiens

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UAS18 Pf var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAS19 from Apac, Uganda originated from a patient sufferring from severe malaria. Erythrocytes were separated from other blood constituents and cultured until parasites matured into trophozoites. Trophozoites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Homo sapiens

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UAS19 Pf var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAS20 from Apac, Uganda originated from a patient sufferring from severe malaria. Erythrocytes were separated from other blood constituents and cultured until parasites matured into trophozoites. Trophozoites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Homo sapiens

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UAS20 Pf var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAS21 from Apac, Uganda originated from a patient sufferring from severe malaria. Erythrocytes were separated from other blood constituents and cultured until parasites matured into trophozoites. Trophozoites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Homo sapiens

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UAS21 Pf var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAS22 from Apac, Uganda originated from a patient sufferring from severe malaria. Erythrocytes were separated from other blood constituents and cultured until parasites matured into trophozoites. Trophozoites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Homo sapiens

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UAS22 Pf var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAS23 from Apac, Uganda originated from a patient sufferring from severe malaria. Erythrocytes were separated from other blood constituents and cultured until parasites matured into trophozoites. Trophozoites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Homo sapiens

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UAS23 Pf var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAS24 from Apac, Uganda originated from a patient sufferring from severe malaria. Erythrocytes were separated from other blood constituents and cultured until parasites matured into trophozoites. Trophozoites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Homo sapiens

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UAS24 Pf var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAS25 from Apac, Uganda originated from a patient sufferring from severe malaria. Erythrocytes were separated from other blood constituents and cultured until parasites matured into trophozoites. Trophozoites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Homo sapiens

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UAS25 Pf var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAS26 from Apac, Uganda originated from a patient sufferring from severe malaria. Erythrocytes were separated from other blood constituents and cultured until parasites matured into trophozoites. Trophozoites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Homo sapiens

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UAS26 Pf var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAS27 from Apac, Uganda originated from a patient sufferring from severe malaria. Erythrocytes were separated from other blood constituents and cultured until parasites matured into trophozoites. Trophozoites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Homo sapiens

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UAS27 Pf var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAS28 from Apac, Uganda originated from a patient sufferring from severe malaria. Erythrocytes were separated from other blood constituents and cultured until parasites matured into trophozoites. Trophozoites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Homo sapiens

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UAS28 Pf var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAS29 from Apac, Uganda originated from a patient sufferring from severe malaria. Erythrocytes were separated from other blood constituents and cultured until parasites matured into trophozoites. Trophozoites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Homo sapiens

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UAS29 Pf var gene cDNA

text: pcrII-TOPO