text: Institute of Developmental Biology, CAS

text: live

text: Generic

text: adult

text: Mus musculus

text: male

characteristic: sex

text: testis

text: Mouse Testis


status: PUBLIC

characteristic: External Id

text: The Samuel Roberts Noble Foundation

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Medicago truncatula genotype A17

tag: attribute

characteristic: cultivar

text: cDNA was prepared from polyA+ enriched RNA from roots harvested at 10, 17, 22, 31 and 38 days post-inoculation with Glomus versiforme. The cDNA was directionally ligated into the Unizap XR vector from Stratagene and packaged using Gigapack III Gold packaging extracts. Plasmids containing cDNA inserts were excised from the recombinant lambda-Zap phage using Ex-assist helper phage and propagated in XLOLR cells.

characteristic: description

text: Roots harvested at 10, 17, 22, 31 and 38 days post-inoculation with Glomus versiforme. The library was made from a mixture of RNA from each of these stages.

characteristic: dev-stage

text: E. coli strain XLOLR

characteristic: lab-host

text: Medicago truncatula/Glomus versiforme mixed EST library

characteristic: organism

text: EcoRI

text: XhoI

text: roots colonized with Glomus versiforme

characteristic: tissue-type

text: MHAM

characteristic: title

text: phagemid

text: pBluescript SK-

characteristic: vector


text: live

text: Generic

text: lung

characteristic: organ

text: Homo sapiens

text: FIBHSVI (Fibroblast cDNA library induced by binding of HSV I)


text: Digital Gene Technologies

text: live

text: Generic

characteristic: cell-line

text: Homo sapiens

text: Homo sapiens MG63


characteristic: External Id

text: National Institute of Agricultural Science and Technology

text: live

text: Generic

text: isolated by differential display from pepper roots infected by Phytophthora capsici

text: 1st-branched growth stage

text: Capsicum annuum

text: root

text: Capsicum annuum root 1st-branched growth stage


text: Otto-von-Guericke University Magdeburg

characteristic: INSDC center name

text: live

text: Generic

text: dimethylfumaric acid induced lymphocytes

characteristic: cell type

text: Homo sapiens

text: Homo sapiens U937


text: Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: mRNA was extracted from eggs and the library was constructed according to the manufacturer's instructions (Uni-Zap XR vector, Stratagene)

text: egg

text: Schistosoma mansoni

text: XhoI

text: EcoRI

text: NMRI

characteristic: strain

text: Schistosoma mansoni, egg

text: phagemid

text: pBlueScript SK


text: Washington University School of Medicine

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Williams

text: This cDNA library was constructed from mRNA isolated from very young seed coats (20-50mgs fresh weight) of greenhouse grown plants. The library was prepared using the Life Technologies pSuperScript cDNA library construction kit. Complementary DNA was synthesized from mRNA using a poly(dT) sequence with a NotI restrictions site. SalI linkers adapters were ligated to the blunt-ended cDNA fragments followed by NotI digestion. The cDNA fragments were directionally cloned into the NotI-SalI restriction site of the pSPORT1 vector. The ligated cDNA fragments were transformed into E.coli ElectroMax DH10B host cells. This library was constructed by Dr. Lila Vodkin and Dr. Anu Khanna.

text: Glycine max

text: NotI

text: SalI

text: very young seed coats of greenhouse grown plants

text: plasmid


text: University of South Alabama

text: live

text: Generic

text: alveolar carcinoma cell

text: Homo sapiens

text: pGEM-T library


text: Incyte Genomics

text: live

text: Generic

text: Pancreas

text: Homo sapiens

text: Human pancreas expression library


text: Institute of Occupational Medicine, Chinese Academy of Preventive Medicine

text: live

text: Generic

text: fibroblast

text: Homo sapiens

text: lung

text: Human cDNA Lung Library


text: Institute of Molecular Biology and Genetics, Mahidol University, Salaya campus

text: live

text: Generic

text: The library was prepared using protocol given by supplier, Stratagene, Inc.

characteristic: description

text: 16-17 g average body weight (farm-raised)

characteristic: dev-stage

text: E.coli XL-1-Blue MRF'

text: Penaeus monodon

text: EcoR I

text: Xho I

text: hemocytes

text: Penaeus monodon's total hemocyte cDNA library (#2)

text: Phage

text: lambdaZAP II


text: UMR INRA/Universite Henri Poincare 1136

text: live

text: Generic

text: The cDNA library was constructed from 1 ug total ectomycorrhiza RNA using the SMART cDNA library Construction kit (#K1051-1, Clontech, Palo Alto, CA, USA) according to the manufacturer's instructions. cDNAs were cloned into EcoRI-digested pBluescript.

text: symbiotic, 4 days after contact

text: Eucalyptus globulus bicostata

characteristic: lab-host

text: ectomycorrhiza

text: Pisolithus tinctorius

characteristic: organism

text: EcoRI

text: mantle and Hartig net hyphae

text: cDNA library of 4-day-old Eucalyptus globulus bicostata-Pisolithus tinctorius ectomycorrhiza

text: pBluescript


text: Ludwig Institute for Cancer Research

text: live

text: Generic

text: A mini-library was made by cloning products derived from ORESTES PCR (U.S. Letters Patent application No. 196,716 - Ludwig Institute for Cancer Research) profiles into the pUC 18 vector. Reverse transcription of tissue mRNA and cDNA amplification were performed under low stringency conditions.

text: Adult

text: Homo sapiens

text: SmaI

text: SmaI

characteristic: title

text: Plasmid


text: Ludwig Institute for Cancer Research

text: live

text: Generic

text: Adult

text: breast

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid

characteristic: vector


text: Ludwig Institute for Cancer Research

text: live

text: Generic

text: Adult

text: colon

characteristic: organ

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: Ludwig Institute for Cancer Research

text: live

text: Generic

text: Adult

text: leiomios

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: Ludwig Institute for Cancer Research

text: live

text: Generic

text: Adult

text: pnet

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: Ludwig Institute for Cancer Research

text: live

text: Generic

text: Adult

text: stomach

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: Ludwig Institute for Cancer Research

text: live

text: Generic

text: Adult

text: uterus

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: live

text: Generic

text: Anchor enzyme: NlaIII~ This SAGE library is from a Cell line induced with synthetic androgen (R1881). The cells were grown in the absence of androgens for three days, and then induced with androgens for six hours. This library was constructed by Dr. Nick Papadopolous. 'Velculescu, V. E., Zhang, L., Vogelstein, B., and Kinzler, K. W. (1995) Serial Analysis of Gene Expression. Science 270, 484-487'

text: Homo sapiens

characteristic: tissue-type


characteristic: External Id

text: Clemson University

text: live

text: Generic

text: Leaves of various stages were shaken in liquid nitrogen, shearing off trichomes. This procedure yielded a mixture of cells which is highly enriched for trichome likely with minor contaminations of other types of leaf cells

text: mixed stages

text: Solanum habrochaites

text: trichome

text: L. hirsutum trichome, Cornell University


text: live

text: Generic

text: Leukocytes

text: Male blood DNA was isolated from one randomly chosen donor and partially digested with a combination of EcoRI and EcoRI Methylase. Size selected DNA was cloned into the pBACe3.6 vector at EcoRI sites.

text: Homo sapiens

text: EcoRI

text: EcoRI

text: Male

characteristic: sex

text: Blood

text: RPCI_11 Human Male BAC Library

text: BAC


text: USDA, ARS, US Meat Animal Research Center

characteristic: INSDC center name

text: live

text: Generic

text: Library made from pool of embryos in spherical and filamentous stages of development (7.5% and 92.5%, respectively, of each stage) as described in Choi et al, Endocrinology 137, 1457-67, 1996.

text: XLOLR

text: Sus scrofa

text: EcoRI

text: XhoI

text: Day 12 whole embryos

text: MARC PBE

text: plasmid

text: pBLUESCRIPT SK-


text: Bernhard Nocht Institute for Tropical Medicine

characteristic: INSDC first public

text: live

text: Generic

text: Entamoeba histolytica

text: HM-1:IMSS; ATCC 30459

text: Entamoeba histolytica HM-1:IMSS

text: lambda ZAP


characteristic: INSDC last update

text: live

text: Generic

text: Anchor enzyme: NlaIII~ Library constructed in the laboratory of Pat Morin (MorinP@grc.nia.nih.gov)

text: ovary

text: Homo sapiens

text: IOSE29 ovarian surface epithelium cell line

text: plasmid


text: Tokyo University of Marine Science and Technology

text: live

text: Generic

text: Seriola quinqueradiata infected with Photobacterium damsela subsp. piscicida

text: Seriola quinqueradiata

text: kidney

text: Seriola quinqueradiata kidney


text: Theodor Bilharz Research Institute

text: live

text: Generic

text: Total mRNA was isolated from meracedia and cDNA expression library was constructed using the Iambda Zap cDNA cloning system, Uni-Zap XR (Stratagen). The phagemid was in vivo excissed in xLI Blue. The cDNA was sequenced using SK forward primer

text: Meracedia

text: Schistosoma mansoni

text: EcoRI

text: XhoI

text: Egyptian

characteristic: strain

text: Schistosoma mansoni meracedia Lambda Zap expression library

text: Phagemid

text: PBluescript


text: Institution of Medical Biology, CAMS

text: live

characteristic: INSDC status

text: Generic

text: lung

text: Mus musculus

text: NFSL


text: Gembloux Agricultural University

text: live

text: Generic

characteristic: NCBI submission model

text: Homo sapiens

text: Female

text: Placenta

text: Placenta Matchmaker cDNA library(Clontech)


text: live

text: Generic

characteristic: NCBI submission package

text: cDNA made by oligo-dT priming. Directionally cloned into CeuI/SceI sites using the following 5' adaptor: taactataacggtcctaaggtagcga and 3' adaptor: tttcattacctctttctccgcaccccacataaa. Average insert size 1.8kb. Library prepared by Edge BioSystems.

text: DH10B (phage-resistant)

text: Homo sapiens

text: SceI

text: CeuI

text: plasmid (chloramphenicol resistant, grown at 30C)


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.4 kb. Library prepared by Life Technologies.

characteristic: description

text: cervix

text: Homo sapiens

text: NotI

text: SalI

text: cervical carcinoma cell line

text: phagemid (ampicillin resistant)


text: Washington University School of Medicine

text: live

text: Generic

text: unfertilized egg

characteristic: cell type

text: PolyA-selected mRNA was prepared from unfertilized Xenopus laevis eggs. The library was constructed in the vector pT7T3-Pac as described in Bonaldo, M.F., Lennon, G. and Soares, M.B. 'Normalization and subtraction: two approaches to facilitate gene discovery', Genome Research 6:791-806, 1996. The first strand synthesis used a NotI-dT18 primer; double stranded cDNAs were ligated to EcoRI adapters, digested with NotI, and directionally cloned into the NotI and EcoRI-digested pT7T3-Pac vector. The library contained approximately 7.2 X 10^5 recombinants, with average insert sizes of 1-1.5 kb.

text: unfertilized egg

characteristic: dev-stage

text: Xenopus laevis

text: EcoRI

text: NotI

text: female

text: unfertilized egg

text: Blackshear/Soares normalized Xenopus egg library

text: Plasmid


text: Weizmann Institute of Science

text: live

text: Generic

text: Cultured placental JAR cells

text: Cultured human placental JAR cells were treated with 3,4- methylenedioxymethamphetamine (MDMA, Ecstasy) for 1-4 hours. mRNA was extracted from untreated and MDMA treated cells, and differential display PCR and subtractive hybridization DD-PCR were used to identify and sequence this cDNA.

text: Homo sapiens

characteristic: organism

text: JAR cells, Homo sapiens


text: Hebei Normal University

text: live

text: Generic

text: Extract total RNA from the tomato suspension cultured cells, then use RT-PCR, and clone the PCR product to pUC19 with two restriction enzymes EcoRI and BamHI.

text: Solanum lycopersicum

text: Tomato suspension cultured cells

characteristic: title


text: National Cancer Institute-FCRDC

text: live

text: Generic

text: Felis catus

text: Heart

text: Feline Heart cDNA Stratagene UniZAPXR #937802


text: National Cancer Institute-FCRDC

text: live

text: Generic

text: Felis catus

text: Male

text: Testis

text: Feline testis cDNA UniZAPXR


text: The Institute For Genomic Research

text: live

text: Generic

characteristic: cultivar

text: Mus musculus

text: EcoRI

text: XhoI

text: adipose

text: pBluescript SK


text: University of Southern California Norris Cancer Center

text: live

text: Generic

text: Macaca mulatta

text: carotid smooth muscle

text: Macaca mulatta carotid smooth muscle


text: live

text: Generic

text: Macaca mulatta

text: balloon-injured carotid smooth muscle

text: Macaca mulatta balloon-injured carotid smooth muscle


text: Pennsylvania State University

text: live

text: Generic

characteristic: cell-line

text: isolated from SKT6 erythroleukemic cells by suppression subtractive hybridization following stimulation with EGF for three hours

text: Mus musculus

text: Mus musculus SKT6-EE372


text: Max-Planck-Institute of Molecular Genetics

text: live

text: Generic

text: cDNA subtraction library specific for invasive trophoblast cloned into the pGEM T-easy vector (Promega)

text: day 8 of gestation

text: E.coli

characteristic: lab-host

text: Mus musculus

text: trophoblast

characteristic: tissue-type

text: Mouse cDNA subtraction library specific for invasive trophoblast

text: plasmid

text: pGEM T-easy

characteristic: vector


characteristic: External Id

text: Clemson University

characteristic: INSDC center name

text: live

text: Generic

text: 7 days post imbibition on water-agar. Mixed stage whole germinating seedlings from seed coat emergence up to two centimeters in seeds not showing obvious signs of germination were discarded.

text: 7 days post imbibtion

text: Solanum lycopersicum

text: whole seedlings

text: tomato germinating seedlings, TAMU

text: phagemid

text: pBlueScript SK(-)


text: Duke University

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Strain CC-125 was grown to mid-log phase in TAP medium, washed twice in TAP-S (sulfur-deficient TAP medium) and incubated in TAP-S. RNA was isolated after 1, 2 and 4 hours. An equal amount of RNA from each time point was pooled and polyA mRNA purified. cDNA was synthesized and directionally cloned into lambda ZAP II (Stratagene) in the EcoRI (5') and XhoI (3') sites. pBluescript II SK- plasmids were excised from the lambda ZAP clones by superinfection with ExAssist (Stratagene) phage.

text: Chlamydomonas reinhardtii

text: EcoRI

text: XhoI

text: CC-125 wild type mt+ 137C

text: C. reinhardtii CC-125 -S, Lambda Zap II

text: plasmid

text: pBluescript II SK-


text: Duke University

text: live

characteristic: INSDC status

text: Generic

text: This library combines cDNAs from nutrient replete (TAP medium, mid-log phase), sulfur-deprived (TAP medium -S for 2 hr), and iron-deprived cells from CC-125. Fe-deprived cells were obtained by either (1) growth in SGII medium at 0.18 micromolar Fe, 2X wash with SGII -Fe and resuspension in SGII -Fe + 80 micromolar bathophenanthroline sulfonic acid (an iron chelator) for 2 hours or (2) growth in SGII medium, 2X wash with SGII -Fe, resuspension in SGII -Fe and continued growth for 48 hours. 1.25 micrograms of each polyA mRNA prep was used to synthesize cDNA which was directionally cloned into lambda ZAP II (Stratagene) in the EcoRI (5') and XhoI (3') sites. pBluescript II SK- plasmids were excised from the lambda ZAP clones by superinfection with ExAssist (Stratagene) phage.

text: Chlamydomonas reinhardtii

text: EcoRI

text: XhoI

text: CC-125 wild type mt+ 137C

characteristic: strain

text: C. reinhardtii CC-125 nutrient replete, -S, -Fe, Lambda Zap II

text: plasmid

text: pBluescript II SK-


text: National University of Singapore

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: embryonal rhabdomyosarcoma

text: rhabdomyosarcoma (RD) cell line

text: The cDNA was prepared using the anchor primer, H-T11G, from Genhunter

text: embryonal

text: Homo sapiens

text: female

characteristic: sex

text: Human rhabdomyosarcoma cDNA


text: National Institute of Agricultural Science and Technology

text: live

text: Generic

text: average insert size:1000 bp;initial pfu:1.100 X 10 6

characteristic: description

text: XL1-Blue MRF', SOLR

text: Antheraea yamamai

text: EcoRI

text: XhoI

text: posterior silkgland cDNA Library

text: Uni-ZAP XR


text: Kinki University

text: live

text: Generic

text: Paramecium tetraurelia

characteristic: organism

text: Paramecium tetraurelia cDNA


text: Institute of Medical Science, University of Tokyo

text: live

text: Generic

text: Homo sapiens

text: Sugano cDNA library

characteristic: title


text: University of Mainz

text: live

text: Generic

text: 2 month old

characteristic: dev-stage

text: Rattus norvegicus

text: female

text: Sprague-Dawley

text: brain

text: Rat brain cDNA


text: Institute of Genetic Diagnosis, the Fourth Military Medical University

text: live

text: Generic

text: DDRT-PCR method used to get differentially expressed genes

text: stomach

characteristic: organ

text: Homo sapiens

text: gastric epithelial cell GES-1

text: DDRT-PCR


text: Institute for Animal Science and Health

text: live

text: Generic

text: Haemonchus contortus

text: SE

text: Haemonchus contortus trans-spliced expression library

text: plasmid


text: live

text: Generic

text: fibroblast

characteristic: cell type

text: Anchor enzyme: NlaIII~ Large T antigen transformed human fibroblasts clones, harvested from skin tissue, that have not reached the cell crisis state. These cells were grown in MEM (Minimum Essential Media) with 10% FBS, 5% CO2 and harvested at passages 4-8.~ Library construction by MicroSAGE adaptation of Velculescu, V. E., Zhang, L.,Vogelstein, B., and Kinzler, K. W. (1995) Serial Analysis of Gene Expression. Science 270, 484-487.~ Tissue supplier: John Murnane~ Library constructed in the laboratory of G. Riggins, M.D., Ph.D. (Duke University).

characteristic: lab-host

text: Homo sapiens

text: skin

text: plasmid


text: live

text: Generic

text: Anchor enzyme: NlaIII~ The library is from a Duke primary tumor called H988. It is a well differentiated, Oligodendroglioma derived from a forty two (42) year-old female. Library constructed in the laboratory of G. Riggins, M.D., Ph.D. (Duke University).

text: adult

text: brain

text: Homo sapiens

text: female

text: primary tumor, well-differentiated oligodendroglioma

characteristic: tissue-type

text: plasmid

characteristic: vector


characteristic: External Id

text: live

text: Generic

text: fibroblast

text: Anchor enzyme: NlaIII~ Large T antigen transformed human fibroblasts clones, harvested from skin tissue, that have survived crisis. These cells were grown in MEM (Minimum Essential Media) with 10% FBS, 5% CO2 and harvested at passages 4-8.~ Library construction by MicroSAGE adaptation of Velculescu, V. E., Zhang, L.,Vogelstein, B., and Kinzler, K. W. (1995) Serial Analysis of Gene Expression. Science 270, 484-487. Tissue supplier: John Murnane~ Library constructed in the laboratory of G. Riggins, M.D., Ph.D. (Duke University).

text: Homo sapiens

text: skin

text: plasmid


characteristic: INSDC first public

text: live

text: Generic

text: Anchor enzyme: NlaIII~ This library is from a Duke primary tumor called H1043. It is a Grade One(1), pilocytic astrocytoma, derived from a thirty four (34) year-old male. Library constructed in the laboratory of G. Riggins, M.D., Ph.D. (Duke University).

text: adult

text: brain

text: Homo sapiens

text: male

text: primary tumor, pilocytic astrocytoma

text: plasmid


characteristic: INSDC last update

text: live

text: Generic

text: Anchor enzyme: NlaIII~ This library is really SAGE_normal_cerebellum

text: adult

text: brain

text: Homo sapiens

text: female

text: cerebellum

text: plasmid


text: The University of Georgia, Department of Plant Biology

characteristic: INSDC center name

text: live

characteristic: INSDC status

text: Generic

text: The library was made from poly-A RNA in the cloning vector lambda ZAP II. Clones to be sequenced were prepared by mass excision.

text: 5-day-old dark-grown seedlings

text: Sorghum bicolor

text: XhoI

text: EcoRI

text: Dark Grown 1 (DG1)

text: Plasmid

text: Lambda Zap


text: live

text: Generic

characteristic: NCBI submission model

text: Mix of 5-week old plants on days 7 & 8 after water was withheld

text: Sorghum bicolor

text: XhoI

text: EcoRI

text: Water-stressed 1 (WS1)

text: Plasmid

text: Lambda Zap


text: National Cancer Institute

text: live

text: Generic

characteristic: NCBI submission package

text: Mesenchymal/Epithelial

text: Restriction Enzymes 1; ApaI, AatII, SphI, NcoI, BstZI, NotI, SacII, and EcoRI SpeI, EcoRI, NotI, BstZI, PstI, SalI, NdeI, SacI, BstXI, and NsiI cDNA fragment PCR-amplified in mRNA differential display analysis; cloned in pGEM-TEasy (Promega); its expression is developmentally regulated during mesenchymal-epithelial conversion in the metanephric kidney.

text: 13 dpc-16dpc

text: Kidney

text: Rattus norvegicus

text: Metanephric mesenchyme

text: Explanted metanephric mesenchyme induced to differentiate into epithelial structures of the nephron ex vivo.

text: Plasmid

text: pGEM-TEasy (Promega).


text: Tongji Medical University

text: live

text: Generic

characteristic: cell-line

text: Prepared using suppression subtractive hybridization (SSH) method

text: Homo sapiens

text: U937 cell line


text: University of California Los Angeles

text: live

text: Generic

text: Human Umbilical Vein Endothelial Cell

text: Homo sapiens

text: EcoRI

text: EcoRI

text: HUVEC Subtracted Library 1

text: Plasmid

text: In Vitrogen T/A Cloning Vector


text: National Institute of Dental and Craniofacial Research

text: live

text: Generic

text: A subtraction cDNA library was constructed from E8.5 mouse craniofacial mRNA subtracted from E13.5 mouse craniofacial mRNA.

characteristic: description

text: embryonic day 8.5

text: Mus musculus

text: ICR

characteristic: strain

text: craniofacial tissues

text: E8.5 mouse craniofacial subtraction cDNA library

text: pT-Adv


text: Simon Fraser University

text: live

text: Generic

text: Plants were grown in a growth chamber (day/night, 16 h/8 h, 25C/15C). Leaves were excised from plants just prior to the beginning of the light period. The cut ends were immersed in water and the leaves were exposed to continuous white light at 25C. Leaves were collected at different times of treatment and RNA was extracted. Differential display was used to study the gene expression in the samples.

text: Poa secunda

characteristic: organism

text: leaves

text: Excised/illuminated big bluegrass leaves

characteristic: title


text: live

text: Generic

text: Anchor enzyme: NlaIII~ Tissue supplier: LNCaP Cells from ATCC~Library Producer: Srivastava S/Xu L, CPDR, USUHS~LNCaP cells (Item Number CRL-1740, American Type Culture Collection, Rockville, MD) were used for generating SAGE libraries. LNCaP cells were maintained in RPMI 1640 (Life Technologies, Inc., Gaithersburg, MD) supplemented with 10% fetal bovine serum (FBS, Life Technologies, Inc., Gaithersburg, MD) and experiments were performed on cells between passages 20 and 30. For the studies of androgen regulation, charcoal/dextran stripped androgen-free FBS (cFBS, Gemini Bio-Products, Inc., Calabasas, CA) was used. LNCaP cells were cultured first in RPMI 1640 with 10% cFBS for 5 days and then stimulated with an non-metabolizable androgen analog R1881 (DUPONT, Boston, MA) 10-8 M for 24 hours (for generating LNCaP-T library). LNCaP cells identically treated but without R1881 treatment served as control (for generating LNCaP-C library). Cells were harvested at indicated time and polyA+ RNA was double-selected with Fast Track kit (Invitrogene) SAGE libraries were generated according to the procedure provided by Dr. Kinzler (Johns Hopkins University School of Medicine, Baltimore, MD). Briefly, biotinylated oligo dT primed cDNA was prepared from five micrograms of polyA+ RNA from treated and control LNCaP cells and biotinylated cDNA was captured on strepravidin coated magnetic beads cDNA bound to the magnetic beads were digested by NlaIII followed by ligation to synthetic linkers containing a site for anchoring enzyme, NlaIII and a site for tagging enzyme BsmF1. The restriction digestion of ligated products with BsmF1 resulted in the capture of 10-11 bp sequences termed as tags representing signature sequence of unique cDNAs. Strategy combining ligation, PCR, enzymatic digestion and gel purification yielded two tags linked together termed as ditags. Ditags were concatamerized, purified and cloned in plasmid pZero cloning vector (Invitrogen, CA). The clones containing concatamers were screened by PCR and sequenced.

text: Homo sapiens

text: NlaIII


text: live

text: Generic

text: Anchor enzyme: NlaIII~ Tissue supplier: LNCaP Cells from ATCC~Library Producer: Srivastava S/Xu L, CPDR, USUHS~LNCaP cells (Item Number CRL-1740, American Type Culture Collection, Rockville, MD) were used for generating SAGE libraries. LNCaP cells were maintained in RPMI 1640 (Life Technologies, Inc., Gaithersburg, MD) supplemented with 10% fetal bovine serum (FBS, Life Technologies, Inc., Gaithersburg, MD) and experiments were performed on cells between passages 20 and 30. For the studies of androgen regulation charcoal/dextran stripped androgen-free FBS (cFBS, Gemini Bio-Products, Inc., Calabasas, CA) was used. LNCaP cells were cultured first in RPMI 1640 with 10% cFBS for 5 days and then stimulated with an non-metabolizable androgen analog R1881 (DUPONT, Boston, MA) 10-8 M for 24 hours (for generating LNCaP-T library). LNCaP cells identically treated but without R1881 treatment served as control (for generating LNCaP-C library). Cells were harvested at indicated time and polyA+ RNA was double-selected with Fast Track kit (Invitrogene). SAGE libraries were generated according to the procedure provided by Dr. Kinzler (Johns Hopkins University School of Medicine, Baltimore, MD). Briefly, biotinylated oligo dT primed cDNA was prepared from five micrograms of polyA+ RNA from treated and control LNCaP cells and biotinylated cDNA was captured on strepravidin coated magnetic beads cDNA bound to the magnetic beads were digested by NlaIII followed by ligation to synthetic linkers containing a site for anchoring enzyme, NlaIII and a site for tagging enzyme BsmF1. The restriction digestion of ligated products with BsmF1 resulted in the capture of 10-11 bp sequences termed as tags representing signature sequence of unique cDNAs. Strategy combining ligation, PCR, enzymatic digestion and gel purification yielded two tags linked together termed as ditags. Ditags were concatamerized, purified and cloned in plasmid pZero cloning vector (Invitrogen, CA). The clones containing concatamers were screened by PCR and sequenced.

text: Homo sapiens

text: NlaIII


text: The Samuel Roberts Noble Foundation

text: live

text: Generic

text: Contains a mixture of very young, developing, mature and senescing leaves.

text: Pooled developmental

characteristic: dev-stage

text: Medicago truncatula

text: leaf

text: Developing leaf

text: Plasmid

text: Lambda Zap


text: The Samuel Roberts Noble Foundation

text: live

text: Generic

text: Four-week old Rhizobium meliloti-inoculated Medicago truncatula roots, containing a mixture of young and old roots and nodules.

text: Pooled developmental

text: Medicago truncatula

text: root

text: Nodulated root

text: Plasmid

text: Lambda Zap


text: The Samuel Roberts Noble Foundation

text: live

text: Generic

text: Total RNA was extracted from non-nodulated roots of plants grown in 1 mM nitrate medium. Samples were taken at four time points (approximately two days, one, two and six weeks post germination) representing early seedling growth to nitrogen limitation.

text: Pooled developmental

text: Medicago truncatula

text: root

text: Developing root

text: Plasmid

text: Lambda Zap


text: The Samuel Roberts Noble Foundation

text: live

text: Generic

text: Contains a mixture of internodal stem segments

text: Pooled developmental

text: Medicago truncatula

text: stem

text: Developing stem

text: Plasmid

text: Lambda Zap


text: Michigan State University

text: live

text: Generic

characteristic: cultivar

text: cDNAs were derived from reverse transcription of pooled mRNA samples from stressed seedlings (germinated for 4 days in 150mM NaCl, 200mM Mannitol, submerged in distilled water and 0.3% Hydrogen peroxide). The cDNA library was generated by directional ligation of the cDNAs in the EcoRI and XhoI sites of Lambda Uni-Zap XR vector (Stratagene). The library was excised as a pBluescript plasmid from Lambda Uni-Zap XR by ExAssist helper phage and plated on SOLR host cells.

text: 4-day germination under stress (salt/NaCl, dehydration/Mannitol and anaerobic stress)

text: SOLR

text: seeds

characteristic: organ

text: Beta vulgaris

text: EcoRI

text: XhoI

text: whole seedlings

text: Sugar Beet germination cDNA library

text: plasmid

text: pBluescript II KS (+) excised from Lambda Uni-Zap XR


text: Albert Einstein College of Medicine of Yeshiva University

text: live

text: Generic

text: Suppression subtractive hybridization was performed between 13 dpc fetal rat liver as a tester and adult rat liver as a driver using PCR-Select cDNA Subtraction Kit (Clontech). Tester and driver cDNA were digested with Rsa I; adaptors were ligated to the tester. Two rounds of subtraction hybridization were performed, followed by two suppression PCR reactions. Subtracted PCR products were ligated into pGEM T-Easy (Promega).

text: embryonic day 13 post-ferilization

text: Rattus norvegicus

text: Sprague-Dawley

text: liver

characteristic: tissue-type

text: Rat pGEM T-Easy Subtracted Library


text: Graduate School of Biotechnology, University of Korea

text: live

text: Generic

text: Degio

text: mature

text: flower

text: Dianthus caryophyllus

text: carnation flower specific cDNA library


text: Kansas State University

text: live

text: Generic

text: Riley X KS224

text: glandular-haired versus non-glandular-haired reciprocal cDNA subtraction with CLONTECH PCR-Select cDNA subtraction.

text: Medicago sativa

text: Leaf and stem

text: glandular-haired subtracted cDNA library


text: Kansas State University

text: live

text: Generic

text: Riley X KS224

text: Medicago sativa

text: Leaf and stem

text: non-glandular-haired subtracted cDNA library


text: Changhai Hospital

text: live

text: Generic

text: lymphocyte

characteristic: cell type

text: Rattus norvegicus

text: female

characteristic: sex

text: Rattus norvegicus F344


characteristic: External Id

text: Johns Hopkins School of Medicine

characteristic: INSDC first public

text: live

text: Generic

text: obtained from differential display analysis

text: Homo sapiens

text: prostate cancer

text: Homo sapiens prostate cancer


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

characteristic: INSDC center name

characteristic: INSDC last update

text: live

text: Generic

text: 1st strand cDNA was prepared from mRNA obtained from pooled mammary gland tumors with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCACTAGTTTTTTTTTTTTTTTTT 3']. Double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library went through one round of normalization, and was constructed by Bento Soares and M. Fatima Bonaldo.

text: DH10B (phage-resistant)

characteristic: lab-host

text: mammary gland

text: Mus musculus

text: NotI

text: EcoRI

text: pooled mammary gland tumors

text: phagemid (ampicillin resistant)

characteristic: vector


text: Washington University School of Medicine

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: cDNA was synthesized from poly mRNA using an oligo-dT primer containing an XhoI site. Following second strand synthesis, EcoRI adapters were ligated to the cDNA and products were size- selected on sephacryl S500. The cDNAs were ligated to EcoRI/ XhoI prepared lambda ZapII (Stratagene). Clones were converted to phagemids by mass excision using ExAssist helper phage and E.coli SOLR cells (Stratagene). Insert sizes range from 0.3-3.0 kb. The library may contain a small percentage of host or bacterial contaminents. NOTE: Many clones lack the 5' EcoRI site but contain the upstream polylinker sites.

text: Tachyzoite

text: SOLR cells

text: Toxoplasma gondii

text: EcoRI

text: XhoI

text: RH (Type I)

text: TgRH*-Tachyzoite cDNA

text: pBluescript SK-


text: Institut National de la Recherche Agronomique

text: live

text: Generic

text: Pinot Noir

text: Non-oriented library

characteristic: description

text: berries harvested at veraison (onset of ripening)

text: Fruit

text: Vitis vinifera

characteristic: organism

text: Eco RI

text: Grape berries Lambda Zap II Library

text: Phage

text: Lambda Zap II


text: Miyazaki University

text: live

text: Generic

text: common name:japanese flounder ; injected with peptidoglycan

text: Paralichthys olivaceus

text: kidney

text: Paralichthys olivaceus Kidney injected with peptidoglycan

characteristic: title


text: Calgene, LLC

text: live

text: Generic

text: isolated by differential screen for ripening specific mRNA

text: Cucumis melo

text: Cucumis melo differential screen


text: Shionogi & Co., Ltd., Shionogi Institute for Medical Science

text: live

text: Generic

text: pheochromocytoma

characteristic: cell-line

text: Rattus norvegicus

text: Rattus norvegicus PC12


text: Kazusa DNA Research Institute

text: live

text: Generic

text: two to six-week old

characteristic: dev-stage

text: Arabidopsis thaliana

text: EcoRI

text: XhoI

text: Columbia

characteristic: strain

text: aboveground organs

text: Arabidopsis thaliana above-ground organ two to six-week old

text: pBluescriptII SK-


text: Okayama University, Barley Germplasm Center

text: live

text: Generic

text: Haruna Nijo

characteristic: cultivar

text: Hordeum vulgare subsp. vulgare

text: Upper three leaves at heading stage

characteristic: tissue-type

text: Hordeum vulgare subsp. vulgare Upper three leaves at heading stage


characteristic: External Id

text: Max Planck Institute of Molecular Plant Physiology

text: live

text: Generic

text: Gifu (B-129)

text: The library was prepared using mRNA extracted from a mixture of mature (pink) and immature (white) nodules of Lotus japonicus ecotype 'Gifu'. Nodules were induced by, and contained Mesorhizobium loti strain NZP2235.

text: Nodule

characteristic: organ

text: Lotus japonicus

text: EcoRI

text: XhoI

text: Lotus japonicus nodule library, mature and immature nodules

text: plasmid

text: pBluescript SK-


characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: Gifu (B-129)

text: The library was prepared using mRNA extracted from nodules of 5 and 7 week-old Lotus plants. Nodules were induced by, and contained Mesorhizobium strain R7A.

text: 5 and 7 week-old plants

text: Nodule

text: Lotus japonicus

text: SalI

text: NotI

text: Lotus japonicus nodule library 5 and 7 week-old

text: plasmid


characteristic: INSDC last update

text: live

text: Generic

text: Williams

text: This cDNA library was constructed from mRNA isolated from immature leaves (unfurled trifoliate) of greenhouse grown plants that were 2 weeks old. The library was prepared using the Life Technologies pSuperScript cDNA library construction kit. Complementary DNA was synthesized from mRNA using a poly (dT) sequence with a NotI restrictions site. SalI linkers adapters were ligated to the blunt-ended cDNA fragments followed by NotI digestion. The cDNA fragments were directionally cloned into the NotI-SalI restriction site of the pSPORT1 vector. The ligated cDNA fragments were transformed into E.coli ElectroMax DH10B host cells. This library was constructed in the laboratory of Dr. Lila Vodkin by Anu Khanna at the University of Illinois at Urbana-Champaign. e-mail: l-vodkin@uiuc.edu

characteristic: lab-host

text: Glycine max

text: NotI

text: SalI

text: immature leaves of greenhouse grown plants

text: plasmid


text: live

characteristic: INSDC status

text: Generic

text: cDNA made by oligo-dT priming. Directionally cloned into EcoRI/XhoI sites using the following 5' adaptor: GGCACGAG(G). Size-selected >500bp for average insert size 1.8kb. Library constructed by Ling Hong in the laboratory of Gerald M. Rubin (University of California, Berkeley) using ZAP-cDNA synthesis kit (Stratagene) and Superscript II RT (Life Technologies).

text: DH10B (phage-resistant)

text: kidney

text: Homo sapiens

text: XhoI

text: EcoRI

text: renal cell adenocarcinoma

text: plasmid (chloramphenicol resistant, grown at 30C)

characteristic: vector


text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: DH10B (phage-resistant)

text: lymph

text: Homo sapiens

text: XhoI

text: EcoRI

text: Burkitt lymphoma


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

characteristic: description

text: DH10B (phage-resistant)

text: placenta

text: Homo sapiens

characteristic: organism

text: XhoI

text: EcoRI

text: choriocarcinoma


text: Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College

text: live

text: Generic

text: Jurkat

characteristic: cell-line

text: The libraries is achieved by using CLONTECH PCR-Select cDNA Subtraction Kit. There are two libraries named the obverse library and the reverse library each. In the obverse library, the tester is s-TRAIL induced Jurkat cells and the driver is healthy Jurkat cells; In the reverse library, the tester is healthy Jurkat cells and the driver is s-TRAIL induced Jurkat cells. All clones differentially expressed are confirmed by the Northern Blot.

text: Homo sapiens

text: EcoRI

text: Subtracted cDNA libraries from human Jurkat cells

characteristic: title

text: Plasmid

text: pGEM-T-Easy Vector


text: Washington University School of Medicine

text: live

text: Generic

text: SL1-Oligo(dT) PCR-based library. Zeldia cDNA PCR products containing SL1 on the 5' end and oligo(dT) on the 3' end were non-directionally cloned into pCRII-TOPO (Invitrogen) following the Topo TA cloning protocol. The cDNA insert can be excised by digestion with EcoRI. This library was constructed by Merry Brook and Dr. Andrew Kloek at Divergence LLC. The strain PDL3 used in this work was provided by the Caenorhabditis Genetics Center at the University of Minnesota.

text: Mixed

characteristic: dev-stage

text: Zeldia punctata

characteristic: strain

text: Kloek Brook Zeldia punctata SL1

text: Ampicillin and Kanamycin resistant

text: pCRII-TOPO


text: live

text: Generic

text: Anchor enzyme: NlaIII~ Library constructed by Riggins laboratory Tissue supplied by Jeffrey Marks, Ph.D.

text: Breast

text: Homo sapiens

text: normal epithelial organoids

characteristic: tissue-type


characteristic: External Id

text: University of Florida College of Veterinary Medicine

text: live

text: Generic

text: The subtracted cDNA library of Fischer 344 rats following parainfluenza type 1 (Sendai) virus infection

text: Rattus norvegicus

text: Male

characteristic: sex

text: Fischer 344

text: Lung

text: subtracted cDNA of Fischer 344 rats following parainfluenza type 1 infection

text: Plasmid

text: pT-Adv


text: The University of Suwon

characteristic: INSDC center name

text: live

text: Generic

text: After size fractionation, the cDNA fragments were cloned into the Lambda Uni-ZAP XR vector. XL1-blue MRF' was infected by the packaged product and cultured on an NZCYM agar medium.

text: XL1-Blue MRF

text: Glycine max

text: EcoRI

text: Xho I

text: long hypocotyls of dark grown seedlings

text: Soybean hypocotyls Lambda Zap library

text: phage

text: Uni-ZAP XR


text: INRA

characteristic: INSDC first public

text: live

text: Generic

text: embryonic

text: brain

characteristic: organ

text: Equus caballus

text: male

text: Horse INRA cDNA Library

text: Plasmid


text: Institute of Genetic Diagnosis, the Fourth Military Medical University

characteristic: INSDC last update

text: live

text: Generic

text: gastric carcinoma cell SGC7901

characteristic: cell type

text: differentially expressed gene sequence from human gastric carcinoma cell SGC7901. The method used is DDRT-PCR. The control cell line is human gastric epithelial cell GES-1.

text: stomach

text: Homo sapiens

text: gastric carcinoma cell SGC7901


text: Dept of Animal Sciences, Univ. of Illinois-UC

text: live

characteristic: INSDC status

text: Generic

text: adult

text: Bos taurus

text: ovary

text: Bos taurus ovary adult


text: John Innes Institute

text: live

text: Generic

characteristic: NCBI submission model

text: four days after infection of Arabidopsis thaliana Ws-eds1-1

text: Hyaloperonospora parasitica

text: Peronospora parasitica cDNA-AFLP fragment


text: Hopital Universitaire de Geneve

text: live

text: Generic

characteristic: NCBI submission package

text: adult/elderly

text: Homo sapiens

text: entorhinal cortex

text: Homo sapiens entorhinal cortex adult/elderly


text: Biomedical Research Institute

text: live

text: Generic

text: Hemocyte

text: B.glabrata BS-90 snails were exposed to miracidia (25/snail) from Schistosoma mansoni for 5 hr. Total RNA was isolated from the hemocytes and first strand cDNA synthesized using an oligo-dT primer-linker (XhoI). Second strand synthesis was followed by the ligation of EcoRI adaptors. Following digestion with XhoI, the completed, directional cDNA was cloned into Uni-ZAP XR Phagemid vector by Stratagene.

characteristic: description

text: Biomphalaria glabrata

text: EcoRI

text: XhoI

text: hermaphrodite

text: Biomphalaria glabrata (BS-90)-exposed Lambda Zap Library

text: phagemid

text: pBluescript SK-

characteristic: vector


text: Biomedical Research Institute

text: live

text: Generic

text: Hemocyte

text: Total RNA was isolated from the hemocytes of unexposed Biomphalaria glabrata (BS-90) snails and first strand cDNA synthesized using an oligo-dT primer-linker (XhoI). Second strand synthesis was followed by the ligation of EcoRI adaptors. Following digestion with XhoI, the completed, directional cDNA was cloned into Uni-ZAP XR Phagemid vector by Stratgene.

text: Laboratory host

characteristic: lab-host

text: Biomphalaria glabrata

characteristic: organism

text: EcoRI

text: XhoI

text: hermaphrodite

text: Biomphalaria glabrata (BS-90)-unexposed Lambda Zap Library

text: phagemid

text: pBluescript SK-


text: Institute for Experimental Medicine RAMS

text: live

text: Generic

text: Homo sapiens

text: placenta

text: Clontech HL1008b

characteristic: title


text: John Innes Centre

text: live

text: Generic

text: Petite Havana

characteristic: cultivar

text: cell suspension cultures harvested 30 min after treatment with the Avr9 peptide from the fungus Cladosporium fulvum; 34.1B tobacco contains Cf-9 resistance gene

text: Nicotiana tabacum


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: 1st strand cDNA was primed with an oligo(dT) primer; double-stranded cDNA was ligated using 5' linker ggccgctat and 3' linker aactggaagcttaatt. Library is size-selected >2.5 kb and average insert size is 3.5 kb. Clones were arrayed from primary plating; non-amplified. Library constructed by X. Ren and L. Stubbs (Lawrence Livermore National Laboratory and DOE Joint Genome Institute, 7000 East Ave, L-453, Livermore, CA 94550).

text: 3 weeks

characteristic: dev-stage

text: thymus

text: Mus musculus

text: NotI

text: PacI

text: mixed

characteristic: strain

text: Ren Stubbs mouse thymus

text: phagemid (ampicillin resistant)


characteristic: External Id

text: live

text: Generic

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCAGCCGTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library constructed and normalized by Bento Soares and M.Fatima Bonaldo.

text: DH10B (phage-resistant)

text: bone (pooled)

text: Mus musculus

text: NotI

text: EcoRI

text: phagemid (ampicillin resistant)


characteristic: INSDC center name

text: live

text: Generic

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCACACTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library is normalized, and was constructed and donated by Bento Soares and M.Fatima Bonaldo (University of Iowa) and R. Hardisty, A. Varela-Carver, P. Mburu and S.D.M. Brown (MRC UK Mouse Genome Centre and Mammalian Genetics Unit, Harwell, UK).

text: newborn

text: inner ear, 170 pooled

text: Mus musculus

text: Not I

text: Eco RI

text: male

characteristic: sex

text: C3H x 101 F1

text: phagemid (ampicillin resistant)


text: Ludwig Institute for Cancer Research

characteristic: INSDC first public

text: live

text: Generic

text: A mini-library was made by cloning products derived from ORESTES PCR (U.S. Letters Patent application No. 196,716 - Ludwig Institute for Cancer Research) profiles into the pUC 18 vector. Reverse transcription of tissue mRNA and cDNA amplification were performed under low stringency conditions.

text: Adult

characteristic: organ

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: Ludwig Institute for Cancer Research

characteristic: INSDC last update

text: live

text: Generic

text: Adult

text: breast

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: Ludwig Institute for Cancer Research

text: live

characteristic: INSDC status

text: Generic

text: Adult

text: colon

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: Institution of Basic Medical Sciences

text: live

text: Generic

characteristic: NCBI submission model

text: Rattus norvegicus

text: Sprague-Dawley

text: hippocampus

characteristic: tissue-type

text: Rattus norvegicus Sprague-Dawley hippocampus


text: Zhejiang University Cancer Institute

text: live

text: Generic

characteristic: NCBI submission package

text: acute myeloid leukemia

characteristic: cell type

text: Homo sapiens

text: Homo sapiens acute myeloid leukemia


text: Incyte Genomics

text: live

text: Generic

text: Heart

text: Homo sapiens

text: Human cardiac muscle expression library


text: Yale Univ. School of Medicine

text: live

text: Generic

text: Burkitt's lymphoma

characteristic: cell-line

text: Obtained by RDA performed with BCR cross-linked BL2 and non-stimulated BL2

characteristic: description

text: BCR Cross-linked

text: Homo sapiens


text: Kazusa DNA Research Institute

text: live

text: Generic

text: synonym: Lotus japonicus

text: two-week old plant

text: Lotus japonicus

characteristic: organism

text: EcoRI

text: XhoI

text: ecotype Miyakojima MG-20

text: whole plant

text: Lotus japonicus young plants (two-week old)

characteristic: title

text: pBluescriptII SK-

characteristic: vector


text: Washington University School of Medicine

text: live

text: Generic

text: Jack

characteristic: cultivar

text: This cDNA library was constructed from mRNA isolated from somatic embryos (age ranging from 2 months to 9 months) cultured on MSD 20. The library was prepared using the Life Technologies pSuperScript cDNA library construction kit. Complementary DNA was synthesized from mRNA using a poly (dT) sequence with a NotI restrictions site. SalI linkers adapters were ligated to the blunt-ended cDNA fragments followed by NotI digestion. The cDNA fragments were directionally cloned into the NotI-SalI restriction site of the pSPORT1 vector. The ligated cDNA fragments were transformed into E.coli ElectroMax DH10B host cells. This library was constructed in the laboratory of Dr. Lila Vodkin by Anu Khanna at the University of Illinois at Urbana-Champaign. e-mail: l-vodkin@uiuc.edu

characteristic: lab-host

text: Glycine max

text: NotI

text: SalI

text: somatic embryos cultured on MSD 20

text: plasmid


characteristic: External Id

text: Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences

characteristic: INSDC center name

text: live

text: Generic

text: From screening of stage-specific expressed genes in spermatogenesis by the method of differential display PCR combined with segmentation technique of the seminiferous tubule.

text: stage-specific expressed ESTs

characteristic: dev-stage

text: Rattus norvegicus

text: male

text: Sprague-Dawley

characteristic: strain

text: testis

text: Rat Lambda gt10 cDNA Library


text: Duke University

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: This library, constructed by John Davies and Jeffrey McDermott, combines cDNAs from CC-1690 cells grown to mid-log phase in TAP (acetate-containing) medium in the light, TAP medium in the dark, HS (minimal) medium in ambient levels of CO2 and HS medium bubbled with 5% CO2. PolyA mRNA was purified from each sample, pooled and cDNA synthesized. The cDNA was directionally cloned into lambda ZAP II (Stratagene) in the EcoRI (5') and XhoI (3') sites. pBluescript II SK- plasmids were excised from the lambda ZAP clones by superinfection with ExAssist (Stratagene) phage.

text: Chlamydomonas reinhardtii

text: EcoRI

text: XhoI

text: CC-1690 wild type mt+ 21gr

text: C. reinhardtii CC-1690, Lambda Zap II

text: plasmid

text: pBluescript II SK-


text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Method of cloning used: directed ligation ; insertCheck: 95.8 blue and 4.2 white. Library constructed by Dr. Bernhard Korn and Sabine Henze. Note: This is a Xenopus Gene Collection (XGC) library.

text: stage 24

text: Xenopus laevis

text: NotI

text: SalI

text: mixed

characteristic: sex

text: whole tadpole

text: Xenopus laevis tadpole stage 24

text: plasmid (ampicillin resistant)


text: live

text: Generic

characteristic: NCBI submission package

text: cDNA made by oligo-dT priming. Library constructed by Dr. Francesca Mariani in the laboratory of R. Harland, Ph.D. (University of California, Berkeley). References: XBF-2 is a transcriptional repressor that converts ectoderm into neural tissue. Mariani, FV. Harland, RM., Development. 1998 Dec;125(24):5019-31. PMID: 9811586; UI: 99030283; Use of large-scale expression cloning screens in the xenopus laevis tadpole to identify gene function. Grammer TC. Liu KJ. Mariani FV. Harland RM,. Dev Biol. 2000 Dec 15;228(2):197-210. PMID: 11112324; UI: 20564075; Note: This is a Xenopus Gene Collection (XGC) library.

text: stage 19-23

text: DH10B (phage-resistant)

text: Xenopus laevis

text: NotI

text: SalI

text: neurula

characteristic: tissue-type

text: Harland stage 19-23

text: plasmid (ampicillin resistant, 200 ug/ml)

text: pCS107 (custom)


text: live

text: Generic

text: mRNA made from normal thyroid epithelium, cDNA made by oligo-dT priming. Non-directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 500 bp. Primary library. cDNA Library Preparation: David B. Krizman, Ph.D. REFERENCE: Krizman et al. (1996) Cancer Research 56:5380-5383.

characteristic: description

text: thyroid

characteristic: organ

text: Homo sapiens

text: normal epithelium

text: plasmid (ampicillin resistant)


text: Chongqing University of Medical Sciences

text: live

text: Generic

text: Homo sapiens

characteristic: organism

text: Human Leukemia K562 Cell Line Express Library

characteristic: title


text: Duke University

text: live

text: Generic

text: This library was constructed by John Davies and Jeffrey McDermott. RNA was isolated from strain CC-2290 (Minnesota isolate of C. reinhardtii) grown to mid-log phase in TAP (acetate containing) medium in the light. PolyA mRNA was purified, and cDNA was synthesized and directionally cloned into lambda ZAP II (Stratagene) in the EcoRI (5') and XhoI (3') sites. pBluescript II SK- plasmids were excised from the lambda ZAP clones by superinfection with ExAssist (Stratagene) phage. The library was normalized using method 4 described in Bonaldo et al (1996) Genome Research 6: 791-806.

text: Chlamydomonas reinhardtii

text: EcoRI

text: XhoI

text: CC-2290 wild type mt- S1 D2

text: C. reinhardtii CC-2290, normalized, Lambda Zap II

text: plasmid

text: pBluescript II SK-

characteristic: vector


text: Fred Hutchinson Cancer Research Center

text: live

text: Generic

text: Avian leukosis virus-induced lymphoma

characteristic: cell type

characteristic: cell-line

text: A cDNA library was prepared using both oligo dT and random primers and cloned into the Eco R1 site of lamda ZAP (strategene). This base library was subtracted with mRNA from normal 2-week bursa, and with an excess of avian leukosis virus, c-myc, and immunoglobulin sequences, by the method of Sive and St. John (A simple subtractive hybridization technique employing photoactivatible biotin and phenol extraction. Nucleic Acids Research 16, 10937, 1988)to produce DT40subNB.

text: bursal stem cell

text: Gallus gallus

text: bursa of Fabricius


characteristic: External Id

text: University of Glasgow

characteristic: INSDC center name

text: live

text: Generic

text: Brugia pahangi

text: mammalian microfilariae SL1


text: Biotechnoloy

text: live

text: Generic

text: Oryza sativa

text: Oryza sativa genes induced by Xanthomonas oryzae pv. oryzae


text: Vavilov Institute of General Genetics

text: live

text: Generic

text: Homo sapiens

text: pUC-derived chromosome 13 enriched cDNA library


text: Institute of Animal Breeding and Husbrandry, Christian-Albrechts-University

text: live

text: Generic

text: epithelial

text: adult, lactating

characteristic: dev-stage

text: Bos taurus

text: female

text: Holstein Friesian

characteristic: strain

text: mammary gland

text: Bovine Lambda ZapII Library


text: Shanghai Institute of Physiology, Chinese Academy of Sciences

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: It was prepared with suppression subtractive hybridization (SSH).

text: adult

text: Rattus norvegicus

text: female

text: Sprague-Dawley

text: brain

text: Rat cDNA library generated with SSH methodology

text: Plasmid

text: T/A Easy Vector


text: Institute of Developmental Biology, CAS

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: adult

text: Mus musculus

text: testis

text: Mus musculus testis adult


text: National Institute of Agricultural Science and Technology

text: live

text: Generic

characteristic: NCBI submission package

text: isolated roots susceptible to Phytophtora capsici

text: Capsicum annuum

text: root

text: Capsicum annuum root susceptible to Phytophtora capsici


text: Stanford University, Department of Biological Sciences

text: live

text: Generic

text: normalized library

text: Gillichthys mirabilis

text: liver

characteristic: tissue-type

text: Gillichthys mirabilis liver library

text: plasmid


text: live

text: Generic

text: normalized library

characteristic: description

text: Gillichthys mirabilis

text: skeletal white muscle

text: Gillichthys mirabilis muscle library

text: plasmid


text: live

text: Generic

text: normalized library

text: Gillichthys seta

text: liver

text: Gillichthys seta liver library

text: plasmid


text: live

text: Generic

text: normalized library

text: Gillichthys seta

text: brain

text: Gillichthys seta brain library

text: plasmid


text: Washington University School of Medicine

text: live

text: Generic

text: This Zebrafish library was constructed by Dr. Bernhard Korn (email: b.korn@dkfz-heidelberg.de). RZPD library number: 717

text: 15-19 hour embryo

text: E.Coli (DH10B/XL2blue)

characteristic: lab-host

text: Danio rerio

text: NotI

text: SalI

text: mixed

characteristic: sex

text: unspecified

text: Zebrafish 15-19hr embryonic cDNA

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: Williams

characteristic: cultivar

text: This cDNA library was constructed from mRNA isolated from fully expanded leaves of greenhouse grown plants that were 2 weeks old. The library was prepared using the Life Technologies pSuperScript cDNA library construction kit. Complementary DNA was synthesized from mRNA using a poly(dT) sequence with a NotI restrictions site. SalI linkers adapters were ligated to the blunt-ended cDNA fragments followed by NotI digestion. The cDNA fragments were directionally cloned into the NotI-SalI restriction site of the pSPORT1 vector. The ligated cDNA fragments were transformed into E.coli Electro- Max DH10B host cells. This library was constructed in the laboratory of Dr. Lila Vodkin by Anu Khanna at the University of Illinois at Urbana- Champaign. email: l-vodkin@uiuc.edu

text: 2 week old

text: Glycine max

characteristic: organism

text: NotI

text: SalI

characteristic: title

text: plasmid


text: live

text: Generic

text: normalized library

text: Gillichthys mirabilis

text: brain

text: Gillichthys mirabilis brain library

text: plasmid

characteristic: vector


characteristic: External Id

text: live

text: Generic

text: Directionally cloned. This library was constructed by Volker Gawantka and Dr. Nicolas Pollet. Note: This is a Xenopus Gene Collection (XGC) library.

text: stage 13 (neurula)

text: E.coli XII Blue F+ (Stratagene) (XII Blue F+ (Stratagene))

text: Xenopus laevis

text: NotI

text: SalI

text: neurula

text: Xenopus embryo stage 13 plasmid

text: phagemid

text: pBluescript KS+


text: University of Maryland, Baltimore

characteristic: INSDC center name

text: live

text: Generic

text: P. yoelii sporozoites were IV injected into BALB/c mice and livers were harvested 24 hours post injection. Total RNA was processed and differential display was performed on the infected liver samples along with uninfected BALB/c liver control.

text: liver

characteristic: organ

text: Mus musculus

text: BALB/c

text: liver

text: Plasmodium yoelii infected liver tissues


text: China Agriculture University

text: live

text: Generic

text: Gallus gallus

text: hypothalamus

text: Chicken hypothalamus cDNA library


text: Institution of Medical Biology, CAMS

text: live

text: Generic

text: Fibroblast cDNA library induced by binding of HBV antigen

text: embryo

characteristic: dev-stage

text: lung

text: Homo sapiens

text: FIBHBVA


text: The Institute of Physical and Chemical Research (RIKEN)

characteristic: INSDC first public

text: live

text: Generic

text: cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and Genome Science Laboratory in RIKEN. Division of Experimental Animal Research in Riken contributed to prepare mouse tissues. 1st strand cDNA was primed with a primer [5' GAGAGAGAGAAGGATCCAAGAGCTCTTTTTTTTTTTTTTTTVN 3'], cDNA was prepared by using trehalose thermo-activated reverse transcriptase and subsequently enriched for full-length by cap-trapper. Second strand cDNA was prepared with the primer adapter of sequence [5' GAGAGAGAGATTCTCGAGTTAATTAAATTAATCCCCCCCCCCCCC 3']. cDNA was cleaved with XhoI and SstI.

text: adult

text: SOLR

text: Mus musculus

text: XhoI

text: SstI

text: male

text: tongue

text: RIKEN full-length enriched, adult male tongue


characteristic: INSDC last update

text: live

text: Generic

text: 13 days embryo

text: SOLR

text: Mus musculus

text: XhoI

text: SstI

text: liver

text: RIKEN full-length enriched, 13 days embryo liver


text: live

characteristic: INSDC status

text: Generic

text: 11 days embryo

text: SOLR

text: Mus musculus

text: XhoI

text: SstI

text: RIKEN full-length enriched, 11 days embryo


text: live

text: Generic

characteristic: NCBI submission model

text: cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and Genome Science Laboratory in RIKEN. Division of Experimental Animal Research in Riken contributed to prepare mouse tissues. 1st strand cDNA was primed with a primer [5' GAGAGAGAGAAGGATCCAAGAGCTCTTTTTTTTTTTTTTTTVN 3'], cDNA was prepared by using trehalose thermo-activated reverse transcriptase and subsequently enriched for full-length by cap-trapper. cDNA went through one round of normalization to Rot = 10.0 and subtraction to Rot = 50.0. Second strand cDNA was prepared with the primer adapter of sequence [5' GAGAGAGAGATTCTCGAGTTAATTAAATTAATCCCCCCCCCCCCC 3']. cDNA was cleaved with XhoI and SstI.

text: 13 days embryo

text: SOLR

text: Mus musculus

text: XhoI

text: SstI

text: head

text: RIKEN full-length enriched, 13 days embryo head


text: Stanford University

text: live

text: Generic

characteristic: NCBI submission package

text: cDNA library from fully differentiated maize tissues from an active Mutator plant. Tissue ratio is 4:2:1:1:1:1 (tassel, kernel, silk, husk, root, leaf). Unidirectionally cloned. New library number given to library 707 for additional sequencing.

text: fully-grown

text: tassel, kernal, silk, husk, root, leaf

text: Zea mays

text: EcoRI

text: tassel, kernal, silk, husk, root, leaf

text: 945 - Mixed adult tissues from Walbot lab, same as 707 (SK)

text: plasmid


text: Carlsberg Laboratory

text: live

text: Generic

text: conidia

characteristic: cell type

text: Hordeum vulgare

text: Blumeria hordei

text: Lambda Zap, Stratagene


text: Ludwig Institute for Cancer Research

text: live

text: Generic

text: A mini-library was made by cloning products derived from ORESTES PCR (U.S. Letters Patent application No. 196,716 - Ludwig Institute for Cancer Research) profiles into the pUC 18 vector. Reverse transcription of tissue mRNA and cDNA amplification were performed under low stringency conditions.

characteristic: description

text: Adult

text: uterus

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: Ludwig Institute for Cancer Research

text: live

text: Generic

text: Adult

text: stomach

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: live

text: Generic

text: Adult

text: stomach

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: Beijing Medical University

text: live

text: Generic

text: isolated from arthritic rat

text: Rattus norvegicus

text: Wistar

characteristic: strain

text: spinal cord horn

characteristic: tissue-type

text: Rattus norvegicus spinal cord horn


text: USDA/ARS, Biosciences Research Lab

text: live

text: Generic

text: 3-day induced (decapitated)

text: Euphorbia esula

text: underground adventitious buds

text: leafy spurge Lambda HybriZAP 2.1 two-hybrid vector cDNA Library


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCAGGATTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library constructed and normalized by Bento Soares and M.Fatima Bonaldo.

text: DH10B (phage resistant)

characteristic: lab-host

text: Mus musculus

text: NotI

text: EcoRI

text: germinal B-cell from resting spleen

text: phagemid (ampicillin resistant)


text: Washington University School of Medicine

text: live

text: Generic

text: This Zebrafish library was constructed by Dr. Susan E. Brockerhoff (email: sbrocker@u.washington.edu) RZPD library number: 760

text: 1-2 years

text: E.Coli XL1-Blue MRF' (XL1-Blue MRF')

text: Danio rerio

characteristic: organism

text: EcoRI

text: SalI

text: mixed

characteristic: sex

text: wild-type

text: Zebrafish adult retina cDNA

text: phagemid (ampicillin resistant)

text: Lambda ZAP II (pBluescript SK-)


text: live

text: Generic

text: mRNA made from normal thyroid epithelium, cDNA made by oligo-dT priming. Non-directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 500 bp. Primary library. cDNA Library Preparation: David B. Krizman, Ph.D. REFERENCE: Krizman et al. (1996) Cancer Research 56:5380-5383.

text: thyroid

text: Homo sapiens

text: normal epithelium

characteristic: title

text: plasmid (ampicillin resistant)

characteristic: vector


characteristic: External Id

characteristic: INSDC center name

text: live

text: Generic

text: mRNA made from thyroid adenoma (benign), cDNA made by oligo-dT priming. Non-directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 500 bp. Primary library. cDNA Library Preparation: David B. Krizman, Ph.D. REFERENCE: Krizman et al. (1996) Cancer Research 56:5380-5383.

text: thyroid

text: Homo sapiens

text: follicular adenoma (benign lesion)

text: plasmid (ampicillin resistant)


text: live

text: Generic

text: mRNA made from normal prostate epithelium, cDNA made by oligo-dT priming. Non-directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 500 bp. Primary library. cDNA Library Preparation: David B. Krizman, Ph.D. REFERENCE: Krizman et al. (1996) Cancer Research 56:5380-5383.

text: prostate

characteristic: organ

text: Homo sapiens

text: male

text: normal epithelium

text: plasmid (ampicillin resistant)


characteristic: INSDC first public

text: live

text: Generic

text: mRNA made from prostatic carcinoma, cDNA made by oligo-dT priming. Non-directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 500 bp. Primary library. cDNA Library Preparation: David B. Krizman, Ph.D. REFERENCE: Krizman et al. (1996) Cancer Research 56:5380-5383.

text: prostate

text: Homo sapiens

text: male

text: carcinoma

text: plasmid (ampicillin resistant)


text: Ludwig Institute for Cancer Research

characteristic: INSDC last update

text: live

text: Generic

text: Adult

characteristic: dev-stage

text: leiomios

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: Beijing Medical University

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Rattus norvegicus

text: Wistar

text: brain

text: Rattus norvegicus Wistar brain


text: National University of Singapore

text: live

text: Generic

characteristic: NCBI submission package

text: isolated from Chinese patient with Nipah virus encephalitis

text: 51 year old

text: Homo sapiens

text: female

text: cerebrospinal fluid

text: Homo sapiens cerebrospinal fluid 51 year old female


text: Ludwig Institute for Cancer Research

text: live

text: Generic

characteristic: description

text: Adult

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: Ludwig Institute for Cancer Research

text: live

text: Generic

text: Adult

text: colon

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: Instituto de Biologia Universidad Nacional de Tucuman, INSIBIO, CONICET-UNT

text: live

text: Generic

text: early pregnant

text: Rattus norvegicus

text: female

text: Wistar

characteristic: strain

text: oviduct

text: early pregnant rat oviduct


text: live

text: Generic

text: ovariectomized

text: Rattus norvegicus

text: female

text: Wistar

text: oviduct

characteristic: tissue-type

text: ovariectomized rat oviduct


text: University of North Carolina Medical School

text: live

text: Generic

text: This subtracted cDNA library was constructed by inserting desired differentially expressed sequences into a plasmid pGEM-T, followed by transforming E. coli strain JM109. Differentially expressed sequences from fasted rat liver were generated by suppression subtractive hybridization, in which mRNA from fasted rats was used as a test and mRNA from control fed rats as a reference. A standard tailing procedure with Taq DNA polymerase was employed to introduce 3'-A overhang to the desired cDNAs before cloning into pGEM-T. Subtraction efficiency of this library was shown to be >60%. The average insert size of clones in this library is 369 bp (20 bp).

text: five-week-old postnatal, fasting for 48 hours

text: liver

text: Rattus norvegicus

text: male

text: Sprague-Dawley

text: Rat Liver Subtracted cDNA Library

text: Plasmid

text: pGEM-T


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.2 kb. Library constructed by Life Technologies.

text: DH10B (phage-resistant)

characteristic: lab-host

text: adrenal gland

text: Homo sapiens

text: NotI

text: SalI

text: neuroblastoma

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.3 kb. Library constructed by Life Technologies.

text: DH10B (phage-resistant)

text: tongue

text: Homo sapiens

characteristic: organism

text: NotI

text: SalI

text: squamous cell carcinoma

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.1 kb. Library constructed by Life Technologies.

text: DH10B (phage-resistant)

text: pancreas

text: Homo sapiens

text: NotI

text: SalI

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.4 kb. Library constructed by Life Technologies.

text: DH10B (phage-resistant)

text: thyroid

text: Homo sapiens

text: NotI

text: SalI

text: follicular carcinoma

text: phagemid (ampicillin resistant)


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: DH10B (phage-resistant)

text: uterus

text: Homo sapiens

text: NotI

text: SalI

text: tumor

text: phagemid (ampicillin resistant)


text: Ludwig Institute for Cancer Research

text: live

text: Generic

text: A mini-library was made by cloning products derived from ORESTES PCR (U.S. Letters Patent application No. 196,716 - Ludwig Institute for Cancer Research) profiles into the pUC 18 vector. Reverse transcription of tissue mRNA and cDNA amplification were performed under low stringency conditions.

text: Adult

text: pnet

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: Ludwig Institute for Cancer Research

text: live

text: Generic

text: Adult

text: ovary

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: Incyte Genomics

text: live

text: Generic

text: Homo sapiens

text: Human matrix tissue expression library


text: INSERM

text: live

text: Generic

text: DDRT-PCR fragments inserted in pGEM-T by conventional T/A cloning

text: embryonic day 14 post-fertilization

text: Rattus norvegicus

text: Wistar

text: intestine

text: Rat differential display RT-PCR

text: Plasmid

text: pGEM-T


text: The Institute for Genomic Research (TIGR; www.tigr.org)

text: live

text: Generic

text: Bintje

characteristic: cultivar

text: RNA was supplied by Christian Bachem & Beatrix Horvath(Laboratory of Plant Breeding, Dept. of Plant Sciences, Wageningen University, The Netherlands). Total RNA was isolated from developing axillary buds of potato nodal stem cuttings cultured on medium for the introduction of tuber formation as described in Bachem et al. (Plant Journal 1996). Tissue samples were taken of stages corresponding to growing stolons and the early stages of tuber formation.

text: 1 to 3 days

text: SOLR

text: Solanum tuberosum

text: axillary buds of stem explants, swelling stolons

text: potato stolon, Cornell University

text: phagemid

text: pBlueScript SK(-)


text: live

text: Generic

text: Anchor enzyme: NlaIII~ This SAGE library is from breast, normal luminar mammary epithelium, BerEp4 purified cells. 'Velculescu, V. E., Zhang, L., Vogelstein, B., and Kinzler, K. W. (1995) Serial Analysis of Gene Expression. Science 270, 484-487'

text: Homo sapiens

text: Breast, normal luminar mammary epithelium, BerEp4 purified cells

characteristic: title


text: live

text: Generic

text: Anchor enzyme: NlaIII~ This SAGE library is from brain, normal thalamus. Post-mortem delay of 3:15 /Velculescu, V. E., Zhang, L., Vogelstein, B., and Kinzler, K. W. (1995) Serial Analysis of Gene Expression. Science 270, 484-487/

text: Homo sapiens

text: Brain, normal thalamus. Post-mortem delay of 3:15


text: live

text: Generic

text: Anchor enzyme: NlaIII~ This SAGE library is a prostate cell line. 'Velculescu, V. E., Zhang, L., Vogelstein, B., and Kinzler, K. W. (1995) Serial Analysis of Gene Expression. Science 270, 484-487'

text: Homo sapiens

text: Prostate, cell line


text: live

text: Generic

text: Anchor enzyme: NlaIII~ This SAGE library is from brain, medulloblastoma grade IV. 'Velculescu, V. E., Zhang, L., Vogelstein, B., and Kinzler, K. W. (1995) Serial Analysis of Gene Expression. Science 270, 484-487'

text: Homo sapiens

text: Brain, medulloblastoma grade IV


text: The Institute for Genomic Research

text: live

text: Generic

text: Estimated insert size approx.1 kb

text: SOLR

text: Rattus norvegicus

text: mix - brain, ovary, placenta, kidney, lung, liver, embryo, heart, muscle, spleen

text: Rat gene index, normalized rat, norvegicus, Bento Soares

text: pBlueScript SK(-)

characteristic: vector


characteristic: External Id

text: Beijing Medical University

text: live

text: Generic

text: 16 week fetus

text: Homo sapiens

text: liver

text: Homo sapiens liver 16 week fetus


text: The Institute of Physical and Chemical Research (RIKEN)

characteristic: INSDC center name

text: live

text: Generic

text: ES cells

characteristic: cell type

text: cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and Genome Science Laboratory in RIKEN. Division of Experimental Animal Research in Riken contributed to prepare mouse tissues. 1st strand cDNA was primed with a primer [5' GAGAGAGAGAAGGATCCAAGAGCTCTTTTTTTTTTTTTTTTVN 3'], cDNA was prepared by using trehalose thermo-activated reverse transcriptase and subsequently enriched for full-length by cap-trapper. cDNA went through one round of normalization to Rot = 5.0 and subtraction to Rot = 25.0. Second strand cDNA was prepared with the primer adapter of sequence [5' GAGAGAGAGATTCTCGAGTTAATTAAATTAATCCCCCCCCCCCCC 3']. cDNA was cleaved with XhoI and SstI.

text: SOLR

text: Mus musculus

text: XhoI

text: SstI

text: RIKEN full-length enriched, ES cells XhoI/SstI


text: Yale School of Medicine

characteristic: INSDC first public

text: live

text: Generic

text: U937 monocyte

text: Obtained by 5' RACE from total RNA of U937 cells

text: Homo sapiens


text: Clemson University

characteristic: INSDC last update

text: live

text: Generic

text: Morex

text: Plants were grown in the greenhouse at the University of California, Riverside (Fenton, SJ Close, TJ Close). Whole spike with awns trimmed were collected at white, green and yellow anther stages (Fenton). Total RNA was prepared from each pool, equal quantities of all three RNA pools were combined, poly(A) RNA was purified from the mixture, one primary unamplified cDNA library was made, and 1 million pfu were in vivo excised to give pBluescript SK(-) cDNA phagemids. These steps were performed in the TJ Close lab (Choi) at the University of California, Riverside. Phagemids were plated and picked at the Clemson University Genomics Institute (CUGI) (Begum, Palmer, Frisch, Atkins and Wing) Plasmid DNA preparations, DNA sequencing and sequence analysis were performed at CUGI (Wing, Yu, Frisch, Henry, Simmons, Oates, Rambo, Main). The sequence has been trimmed to remove vector sequence and contains a minimum of 100 bases of phred value 20 or above. For more details on library preparation and sequence analysis see http://www.genome.clemson.edu/projects/barley. To order this clone see http://www.genome.clemson.edu/orders Also see Close TJ, Wing R, Kleinhofs A, Wise R (2001) Genetically and physically anchored EST resources for barley genomics. Barley Genetics Newsletter 31:29-30. (http://wheat.pw.usda.gov/ggpages/bgn/31/cover.html)

text: SOLR

text: Hordeum vulgare subsp. vulgare

text: pre-anthesis spike

text: Hordeum vulgare pre-anthesis spike EST library HVcDNA0008 (white to yellow anther)

text: Phage

text: lambdaZAP


text: University of Durham

text: live

text: Generic

text: epithelial lymphocyte

text: adult

characteristic: dev-stage

text: Xenopus laevis

text: intestine

text: Xenopus laevis intestine adult


text: Ludwig Institute for Cancer Research

text: live

characteristic: INSDC status

text: Generic

text: A mini-library was made by cloning products derived from ORESTES PCR (U.S. Letters Patent application No. 196,716 - Ludwig Institute for Cancer Research) profiles into the pUC 18 vector. Reverse transcription of tissue mRNA and cDNA amplification were performed under low stringency conditions.

text: Adult

characteristic: organ

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: The Institute for Genomic Research

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Homo sapiens

text: MAGE resequences, MAGA

text: pBluescriptSKm


text: Ludwig Institute for Cancer Research

text: live

text: Generic

characteristic: description

text: Adult

text: marrow

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: The Institute for Genomic Research

text: live

text: Generic

text: Homo sapiens

text: MAGE resequences, MAGB

text: pBluescriptSKm


text: The Institute for Genomic Research

text: live

text: Generic

text: Homo sapiens

text: MAGE resequences, MAGC

text: pBluescriptSKm


text: The Institute for Genomic Research

text: live

text: Generic

text: Homo sapiens

text: MAGE resequences, MAGD

text: pBluescriptSKm


text: The Institute for Genomic Research

text: live

text: Generic

text: Homo sapiens

text: MAGE resequences, MAGE

text: pBluescriptSKm


text: The Institute for Genomic Research

text: live

text: Generic

text: Homo sapiens

text: MAGE resequences, MAGF

text: pBluescriptSKm


text: The Institute for Genomic Research

text: live

text: Generic

text: Homo sapiens

text: MAGE resequences, MAGG

text: pBluescriptSKm


text: The Institute for Genomic Research

text: live

text: Generic

text: Homo sapiens

text: MAGE resequences, MAGH

text: pBluescriptSKm


text: The Institute for Genomic Research

text: live

text: Generic

text: Homo sapiens

text: MAGE resequences, MAGI

text: pBluescriptSKm


text: The Institute for Genomic Research

text: live

text: Generic

text: Homo sapiens

text: MAGE resequences, MAGJ

text: pBluescriptSKm


text: The Institute for Genomic Research

text: live

text: Generic

text: Homo sapiens

text: MAGE resequences, MAGK

text: pBluescriptSKm


text: The Institute for Genomic Research

text: live

text: Generic

text: Homo sapiens

text: MAGE resequences, MAGL

text: pBluescriptSKm


text: The Institute for Genomic Research

text: live

text: Generic

text: Homo sapiens

text: MAGE resequences, MAGM

text: pBluescriptSKm


text: The Institute for Genomic Research

text: live

text: Generic

text: Homo sapiens

text: MAGE resequences, MAGN

text: pBluescriptSKm


text: The Institute for Genomic Research

text: live

text: Generic

text: Homo sapiens

text: MAGE resequences, MAGO

text: pBluescriptSKm


text: The Institute for Genomic Research

text: live

text: Generic

text: Homo sapiens

text: MAGE resequences, MAGP

text: pBluescriptSKm


text: University at Cologne

text: live

text: Generic

text: embryo, 2-14 hours

text: Drosophila melanogaster

text: Canton-S

characteristic: strain

text: Stratagene Uni-ZAP XR library, Cat. 937602


text: Ludwig Institute for Cancer Research

text: live

text: Generic

text: Adult

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: live

text: Generic

text: Adult

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: Norman Bethune University of Medical Science

text: live

text: Generic

text: fetal

text: brain

text: Homo sapiens

text: Human fetal brain cDNA


text: live

text: Generic

text: Cervus nippon

text: Sika Deer Blood cDNA


text: live

text: Generic

text: Cervus nippon

text: hepatic tissue

characteristic: tissue-type

text: Sika Deer hepatic tissue cDNA


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: cDNA made by oligo-dT priming. Directionally cloned into EcoRI/XhoI sites using the following 5' adaptor: GGCACGAG(G). Size-selected >500bp for average insert size 1.8kb. Library constructed by Ling Hong in the laboratory of Gerald M. Rubin (University of California, Berkeley) using ZAP-cDNA synthesis kit (Stratagene) and Superscript II RT (Life Technologies).

text: DH10B (phage-resistant)

characteristic: lab-host

text: cervix

text: Homo sapiens

characteristic: organism

text: XhoI

text: EcoRI

text: carcinoma cell line

text: plasmid (chloramphenicol resistant, grown at 30C)


text: live

text: Generic

text: DH10B (phage-resistant)

text: ovary

text: Homo sapiens

text: XhoI

text: EcoRI

text: adenocarcinoma cell line

characteristic: title


text: live

text: Generic

text: Anchor enzyme: NlaIII~ As a step toward understanding the complex differences between normal and cancer cells in humans, gene expression patterns were examined in gastrointestinal tumors and normal colon tissues. A frozen tumor sample was obtained from a colon cancer patient. Histopathology confirmed that the tumor sample contained at least 70% of tumor cells. PolyA RNA was isolated and SAGE was performed. More than 300,000 transcripts derived from at least 45,000 different genes were analyzed. Although extensive similarity was noted between the expression profiles, more than 500 transcripts that were expressed at significantly different levels in normal and neoplastic cells were identified. These data provide insight into the extent of expression differences underlying malignancy and reveal genes that may prove useful as diagnostic or prognostic markers.

text: Homo sapiens

text: NlaIII


text: University of Illinois at Urbana-Champaign

text: live

text: Generic

text: The library was prepared by Stratagene using oligo-T priming and unidirectional cloning with an adaptor at the 5' end (GGCACGAG) following the EcoRI site. The mRNA was prepared from antennae of late pupal and newly eclosed female moths. Clones were subcloned in vivo in mass into pBluescript maintained in SOLR cells for DNA sequencing.

text: newly eclosed adults and pharate adults

text: XL1 Blue MRF' and SOLR

text: antennae

text: Manduca sexta

text: EcoRI

text: XhoI

text: female

characteristic: sex

text: Manduca sexta female antennae Uni-ZAP XR library

text: lambda virus

text: Uni-ZAP XR

characteristic: vector


characteristic: External Id

text: live

text: Generic

text: Anchor enzyme: NlaIII~ This SAGE library is from uninduced 293 cells. Approximately 90% of the clones contain inserts and the average insert size is 900kb. 'Velculescu, V. E., Zhang, L., Vogelstein, B., and Kinzler, K. W. (1995) Serial Analysis of Gene Expression. Science 270, 484-487'

text: Homo sapiens

text: Uninduced 293 cells


text: Washington University School of Medicine

characteristic: INSDC center name

text: live

text: Generic

text: 1st strand cDNA was prepared from zebrafish(C32) fin, and was then primed with a Not I - oligo(dT) primer. Double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library is non-normalized. Library was constructed by Ning Wu. NOTE: This clone is available royalty-free through LLNL; contact the IMAGE Consortium (info.llnl.gov)for further information

text: GeneHogs (HS996, a phage-resistant isolate of DH10B)

text: Danio rerio

text: EcoRI

text: NotI

text: Fin

text: Zebrafish Research Genetics C32 fin

text: Phagemid (ampicillin resistant)


text: Duke University

characteristic: INSDC first public

text: live

text: Generic

text: This library, constructed by John Davies and Jeffrey McDermott, combines cDNAs from CC-1690 cells grown to mid-log phase in TAP (acetate-containing) medium in the light, TAP medium in the dark, HS (minimal) medium in ambient levels of CO2 and HS medium bubbled with 5% CO2. PolyA mRNA was purified from each sample, pooled and cDNA synthesized. The cDNA was directionally cloned into lambda ZAP II (Stratagene) in the EcoRI (5') and XhoI (3') sites. pBluescript II SK- plasmids were excised from the lambda ZAP clones by superinfection with ExAssist (Stratagene) phage. The library was normalized using method 4 described in Bonaldo et al (1996) Genome Research 6: 791-806.

text: Chlamydomonas reinhardtii

text: EcoRI

text: XhoI

text: CC-1690 wild type mt+ 21gr

text: C. reinhardtii CC-1690, normalized, Lambda Zap II

text: plasmid

text: pBluescript II SK-


characteristic: INSDC last update

text: live

text: Generic

text: st strand was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCGAATCTTTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library went through one round of normalization, and was constructed by Bento Soares and M. Fatima Bonaldo (University of Iowa). Note: This is a Xenopus Gene Collection (XGC) library.

text: DH10B (phage-resistant)

text: Xenopus laevis

text: NotI

text: EcoRI

text: Soares NXEG

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: Merozoites were obtained from ceacal scrapings of chickens infected with E. tenella. cDNA was synthesized from poly mRNA using an oligo-dT primer containing a XhoI site. Following second strand synthesis, EcoRI adapters were ligated to the cDNA and products were size-selected on Sephacryl S500. The cDNAs were ligated to EcoRI/XhoI prepared lambda ZAPII(Stratagene). Clones were converted to phagemids by mass excision using ExAssist helper phage and E.coli SOLR cells (Stratagene). Insert sizes range from 0.7-1.5Kb. The library may contain a small percentage of host or bacterial contaminants. Clones were selected by negative hybridization against a pool of over-represented ESTs (N>=10, from 1506 previous reads).

text: Merozoite

characteristic: dev-stage

text: SOLR E. coli

text: Eimeria tenella

text: EcoRI

text: XhoI

text: Eimeria M5-6 Merozoite stage subtracted

text: phagemid (Amp. resistant)

text: Bluescript SK-


text: live

characteristic: INSDC status

text: Generic

text: grown in hydroponics, stress 400 mM NaCl (in 0.5 Hoagland's), 30 h

text: 5-6 weeks

text: Mesembryanthemum crystallinum

text: primary leaves

text: MB


text: live

text: Generic

text: grown in hydroponics (0.5 Hoagland's), no stress, harvest at daytime

text: 5-6 weeks

text: Mesembryanthemum crystallinum

text: roots

text: MC


text: University of Arizona

text: live

text: Generic

text: grown in hydroponics, stress 400 mM NaCl (in 0.5 Hoagland's), 78 h stress

text: 5-6 weeks

text: Mesembryanthemum crystallinum

text: roots

text: MH


text: University of Arizona

text: live

text: Generic

text: Columbia

characteristic: cultivar

text: 200mM NaCl

text: 2-3 weeks

text: Arabidopsis thaliana

text: leaves, seedlings

text: AB


text: University of Arizona

text: live

text: Generic

characteristic: NCBI submission model

text: no stress

text: 5 weeks

text: Mesembryanthemum crystallinum

text: apical meristem and leaf primordia

text: MO


text: University of Arizona

text: live

text: Generic

characteristic: NCBI submission package

text: Columbia

text: 20 h 150mM NaCl

text: 2-3 weeks

text: Arabidopsis thaliana

text: seedlings leaf and root

text: AC


text: University of Arizona

text: live

text: Generic

text: 6 weeks in 500mM NaCL

characteristic: description

text: >12 weeks

text: Mesembryanthemum crystallinum

text: flowers and developing seedpods

text: ML


text: live

text: Generic

text: 5-6 weeks

text: Mesembryanthemum crystallinum

text: primary leaves

text: MA


text: live

text: Generic

text: grown in hydroponics, stress 400 mM NaCl (in 0.5 Hoagland's), 1 h stress

text: 5-6 weeks

text: Mesembryanthemum crystallinum

text: roots

text: MD


text: University of Arizona

text: live

text: Generic

text: 6-7 d stress at 500mM NaCl

text: 6 weeks

text: Mesembryanthemum crystallinum

text: primary leaves

text: MK


text: University of Arizona

text: live

text: Generic

text: grown in hydroponics, stress 400 mM NaCl (in 0.5 Hoagland's); 6 h stress

text: 5-6 weeks

text: Mesembryanthemum crystallinum

text: roots

text: ME


text: University of Arizona

text: live

text: Generic

text: 5 d stress at 500mM NaCl

text: 8-9 weeks

text: Mesembryanthemum crystallinum

text: secondary leaves

text: MJ


text: Washington University

text: live

text: Generic

text: Columbia

text: 20 h 200mM NaCl

text: 12 weeks

text: Arabidopsis thaliana

text: leaves, flowering plants

text: AA


text: University of Arizona

text: live

text: Generic

text: 3 d stress at 250 mM NaCl

text: 14 days

text: Mesembryanthemum crystallinum

text: seedlings

text: MI


text: University of Arizona

text: live

text: Generic

text: 3 d 500mM NaCl

text: 6 weeks

text: Mesembryanthemum crystallinum

text: side shoots

text: MN


text: University of Arizona

text: live

text: Generic

text: 3 d 500mM NaCl

text: 6 weeks

text: Mesembryanthemum crystallinum

text: apical meristem and leaf primordia

text: MP


text: University of Arizona

text: live

text: Generic

text: grown in hydroponics, stress 400 mM NaCl (in 0.5 Hoagland's), 30 h stress

text: 5-6 weeks

text: Mesembryanthemum crystallinum

text: roots

text: MG


text: live

text: Generic

text: mRNA made from thyroid adenoma (benign), cDNA made by oligo-dT priming. Non-directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 500 bp. Primary library. cDNA Library Preparation: David B. Krizman, Ph.D. REFERENCE: Krizman et al. (1996) Cancer Research 56:5380-5383.

text: thyroid

characteristic: organ

text: Homo sapiens

text: follicular adenoma (benign lesion)

text: plasmid (ampicillin resistant)


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.4 kb. Library constructed by Life Technologies.

text: DH10B (phage-resistant)

text: ovary

text: Homo sapiens

text: NotI

text: SalI

text: serous papillary tumor

characteristic: tissue-type

text: phagemid (ampicillin resistant)


text: Cold Spring Harbor Laboratory

text: live

text: Generic

text: Resistance: Ampicillin; Autoexcision: pBluescript SK (+/-); Titer: 7 x 10e-9 pfu/mL (as of 9/28/94); Library source: John Doebley, B73

text: Zea mays

text: XhoI

text: EcoRI

text: Maize Glume cDNAs Library

text: Lambda Zap II (Stratagene)


text: USDA, ARS

text: live

text: Generic

text: Marsh

text: 2-5 gram fruit

text: Fruit

text: Citrus x paradisi

text: Flavedo

text: Marsh grapefruit young flavedo


text: Department for Biochemistry

text: live

text: Generic

text: spores

text: Gigaspora rosea

text: asexual

characteristic: strain

text: Gigaspora rosea Lambda Zap Library


text: live

text: Generic

text: A mini-library was made by cloning products derived from ORESTES PCR (U.S. Letters Patent application No. 196,716 - Ludwig Institute for Cancer Research) profiles into the pUC 18 vector. Reverse transcription of tissue mRNA and cDNA amplification were performed under low stringency conditions.

text: Adult

text: breast

text: Homo sapiens

characteristic: organism

text: SmaI

text: SmaI

text: Plasmid


text: Ludwig Institute for Cancer Research

text: live

text: Generic

text: Adult

text: breast

text: Homo sapiens

text: SmaI

text: SmaI

characteristic: title

text: Plasmid


text: University of Kentucky

text: live

text: Generic

text: SN3 merozoites of Sarcocystis neurona were propagated in bovine turbinate cells. cDNAs were synthesized from poly(A)+ RNA by oligo d(T) priming and directionally cloned into the Uni-ZAP XR lambda vector. WARNING: the library contains a small percentage of cDNAs derived from the bovine host cells.

text: merozoites

text: XL1-Blue MRF'

characteristic: lab-host

text: Sarcocystis neurona

text: EcoRI

text: XhoI

text: cSn.1 S. neurona cDNA

text: phage

text: Lambda ZAP II


text: Children's Memorial Research Center

text: live

text: Generic

text: The UI-R-BS1 library is derived from 13 dpc whole embryo tissue. For a detailed description of the library from which this clone was derived, please visit our web site at ratest.eng.uiowa.edu.

text: embryonic 13 dpc

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid

characteristic: vector


characteristic: External Id

text: Children's Memorial Research Center

text: live

text: Generic

text: The UI-R-CA0 library is a subtracted library derived from the following tissues: thalamus, cerebellum, hypothalamus, medulla, pons, midbrain, cerebral cortex, corpus striatum, testis, and hippocampus. For a detailed description of the library from which this clone was derived, please visit our web site at ratest.eng.uiowa.edu. The subtraction has been previously described in (Bonaldo, Lennon and Soares, Genome Research 6:791-806, 1996)

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid


text: United States Army Medical Research Institute of Chemical Defense (USAMRICD)

characteristic: INSDC center name

text: live

text: Generic

text: primary keratinocyte

characteristic: cell type

text: Clonetics 4075

characteristic: cell-line

text: Library preparation: Sequence isolated using PCR-select (Clontech) subtraction library construction (Diatchenko et al., (1996) Proc. Natl. Acad. Sci. USA 93: 6025-6030) after sulfur mustard exposure of primary human adult epidermal keratinocytes. Subtraction library cloned for random sequence selection into pT-Adv vector.

text: adult

text: E. coli (ampicillin-resistant)

text: Homo sapiens

text: female

characteristic: sex

text: skin

text: Human Epidermal Keratinocyte Subtraction Library- Upregulated Transcripts

text: Plasmid

text: pT-Adv


text: Stanford University

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: George Chuck dissected immature tassels between 1mm and 3mm. Sharon Stanfield prepared the cDNA library in HybriZAP. Sample insert size range was 350 bp to 3 Kb with a 1 Kb average.

text: just after the transition from vegetative to inflorescence development

characteristic: dev-stage

text: XLOLR

text: tassels

text: Zea mays

text: EcoRI

text: XhoI

text: tassels

text: 946 - tassel primordium prepared by Schmidt lab

text: phagemid

text: HybriZAP


text: Northwestern Sci-Tech University of Agriculture and Forestry

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: cumulus oocyte complex

text: Sequences obtained by DDRT-PCR method

characteristic: description

text: culture in vitro for 0 hour

text: Bos taurus

text: female

text: ovarium


text: live

text: Generic

text: cumulus oocyte complex

text: Sequences obtained by DDRT-PCR method

text: culture in vitro for 24 hour

text: Bos taurus

text: female

text: ovarium

characteristic: tissue-type


text: The University of Tokyo

text: live

text: Generic

text: fibroblast-like

text: Oryzias latipes

characteristic: organism

text: male

text: HNI

characteristic: strain

text: fin

text: Oryzias latipes HNI-1


text: Genesis Research and Development Corporation Limited

text: live

text: Generic

text: dermal papilla

text: Rattus norvegicus

text: Dark-Agouti

text: vibrissae

text: Rat Lambda ZAP Express Library

characteristic: title


text: Kazusa DNA Research Institute

text: live

text: Generic

text: Neopyropia yezoensis

text: EcoRI

text: XhoI

text: Porphyra yezoensis TU-1

text: pBluescriptII SK-

characteristic: vector


characteristic: External Id

text: Graduate School of Medicine, Kyoto University

text: live

text: Generic

text: Rattus norvegicus

text: kidney

text: Rattus norvegicus kidney


text: Hepatology Institute of Peking University

characteristic: INSDC center name

text: live

text: Generic

text: liver

characteristic: organ

text: Homo sapiens

text: Human Liver MATCHMAKER cDNA Library(cat. #HL4024AH)


text: Ludwig Institute for Cancer Research

text: live

text: Generic

text: Adult

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: live

text: Generic

text: Adult

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: live

text: Generic

text: epithelial

characteristic: cell type

characteristic: cell-line

text: Anchor enzyme: NlaIII~ Library producer: Aldaz Laboratory~MCF7 cells were grown in conditions devoid of estrogen for 48h - (IMEM media supplemented w/ 10% Charcoal Stripped Serum) at time 0h cells were treated with 10-8 M estradiol (E2) or vehicle - This SAGE library represents the 3h time-point of vehicle treatment

text: Top10 bacteria

characteristic: lab-host

text: Breast

text: Homo sapiens

text: NlaIII

text: BsmF1 as tagging enzyme

text: Female

text: Breast Carcinoma - Pleaural effusion

text: plasmid

text: pZero