text: Institut National de la Recherche Agronomique

text: live

text: Generic

text: Shiraz

text: Oriented library, construction described in Generation of ESTs from grape Berry (skin, pulp or seeds) at various developmental stages by Terrier,N., Ageorges,A., Abbal,P., Romieu,C. in J. Plant Physiol. 158 (12): 1575-83 2001

text: Veraison stage

text: Fruit without seeds

text: Vitis vinifera

text: Eco RI

text: XhoI

text: Veraison Grape Berries Lambda Zap II Library

text: Phage

text: Lambda Zap II


status: PUBLIC

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Shiraz

tag: attribute

characteristic: cultivar

characteristic: description

text: Ripe stage

characteristic: dev-stage

text: Fruit without seeds

characteristic: organ

text: Vitis vinifera

characteristic: organism

text: Eco RI

text: XhoI

text: Ripe Grape berries Lamda Zap II Library

characteristic: title

text: Phage

text: Lambda Zap II

characteristic: vector


text: University of Kentucky

text: live

text: Generic

text: Products of differential subtraction by Representational Difference Analysis were digested by DpnII and cloned.

text: Pre-adult to adult

text: Olfactory organ

text: Panulirus argus

text: male and female

characteristic: sex

text: Proximal proliferation zone

characteristic: tissue-type

text: Lobster olfactory proliferation zone enriched

text: Plasmid

text: pBluescript


text: CSIRO Livestock Industries

text: live

text: Generic

text: Arrayed library filters screened by hybridization with labeled dinucleotide repeats. first strand synthesis Xba1-(dT)15 primed, EcoRl adaptors were ligated to cDNA before cloning. EcoRl- Notl- Sall adaptor sequence;5'-AATTCGCGGCCGCGTCGAC GCGCCGGCGCAGCTG-PO4-5' 5' sequencing primer 5' TCCGAGATCTGGACGAGC 3' 3' sequencing primer 5' TAATACGACTCACTATAGGG 3' submitted sequences in excess of 50 bp after vector and quality clipping(phred 13) submitted to GenBank.

text: Adult

characteristic: lab-host

text: midflank skin

text: Ovis aries

text: EcoRI

text: male

text: Merino

characteristic: strain

text: ad adult sheep skin library

text: phagemid

text: pTriplEX


characteristic: External Id

text: CSIRO Livestock Industries

characteristic: INSDC center name

text: live

text: Generic

text: 103-105d pc fetus

text: midflank skin

text: Ovis aries

text: EcoRI

text: male

text: Merino

text: fs 103-105d fetal sheep skin library

text: phagemid

text: pTriplEX


text: CSIRO Livestock Industries

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: 73-75d pc fetus

text: midflank skin

text: Ovis aries

text: EcoRI

text: male

text: Merino

text: fp 73-75d fetal sheep skin library

text: phagemid

text: pTriplEX


text: CSIRO Livestock Industries

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Sheep mid flank skin poly-A+ selected RNA was reverse transcribed with MMLV RT. First strand synthesis initiated with XhoI oligo dT linker (GAGAGAGAGAGAGAGAGAGAACTAGTCTCGAGT18). Submitted sequences vector and quality clipped (phred 13)

text: Adult

text: SURE cells

text: midflank skin

text: Ovis aries

text: EcoRI

text: XhoI

text: male

text: Merino

text: et adult sheep skin library

text: phagemid

text: pBluescript SK-


text: Kansas State University

text: live

text: Generic

characteristic: NCBI submission package

text: Chinese Spring

characteristic: cultivar

text: 3-10 mm

text: immature spikelet

text: Triticum aestivum

text: Chinese Spring wheat immature spikelet cDNA

text: Phage

text: Lambda ZAP


text: UC Davis, Plant Pathology

text: live

text: Generic

text: Cabernet Sauvignon

text: CAB2 is a cDNA library of Vitis vinifera cv. 'Cabernet Sauvignon' Clone 8 berries. Samples were collected at full bloom (80 to 100% flowers showing dehiscence of calyptras or caps and anthers fully extended). Sampled vines were located at the University of California, Davis, Experimental Vineyard. cDNAs were made by oligo-dT priming and directionally cloned. 5'and 3' adaptors were used in cloning as follows: 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' and 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)NN-3'. Library was constructed using the Clontech Creator SMART kit and size-selected to contain the 0.5-3 kb size fraction.

characteristic: description

text: Bloom

characteristic: dev-stage

text: Flower - Bloom

text: Vitis vinifera

text: SfiI

text: SfiI

text: Hermaphrodite

text: Cabernet Sauvignon Flower bloom - CAB2

text: Plasmid

text: pDNR


text: Stanford University

text: live

text: Generic

text: Maize Gene Discovery Project contracted with Invitrogen to produce a normalized, full length library in a pSport vector. This is a Gateway compatible vector, permitting clone movement to new vector backbones for expression in diverse host cells using recombination rather than restriction enzymes. Details of the vector and sequencing primers are available at ZmDB in the EST library description tables. poly(A)+ mRNA was prepared by Invitrogen, and equimolar amounts of RNA from each of the 12 tissue samples were mixed together for selection of mRNA with a 5' cap. After synthesis of cDNA, a normalization step was conducted against the mixture of RNA sources. This step effected a 20X to 80X reduction in common transcript types. Tissues prepared: 1. just emerging silks; 2. inner husks from ears of sample #1; 3. 20 dap aleurone; 4. immature tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day old seedlings; all leaves with an expanded or partially expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 cm long root tips from 15 day old seedlings; 10. 10 dap whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap endosperm and embryo. All of the sequenced clones in project 3530 will be archived at the University of Arizona along with the Unigene clones from the Maize Gene Discovery EST sequencing projects. Clones can be ordered through the ZmDB web site or directly from the University of Arizona (http://www.genome.arizona.edu/orders/). High density filters containing over 18,000 clones can also be ordered from the University of Arizona.

text: varies by tissue

text: silks, husks, ears, pollen, shoot tips, leaf, root tips, whole seed, embryo

characteristic: organ

text: Zea mays

characteristic: organism

text: EcoRV

text: NotI

text: multiple

text: 3530 - Full length cDNA library created by Invitrogen from multiple tissues

text: phagemid

text: pCMV-SPORT 6.1


text: CNRS

text: live

text: Generic

text: Caecal tonsil

text: Gallus gallus

text: Eimeiria tenella-infected caecal tonsil

characteristic: title

characteristic: vector


text: North Carolina State University

text: live

text: Generic

text: 5th stadium, day 1 and 2

text: Digestive System

text: Trichoplusia ni

text: EcoRI

text: XhoI

text: Shorey, University of California at Riverside

text: Trichoplusia ni fifth instar digestive system cDNA library

text: phagemid

text: pBK-CMV


text: Auburn University

text: live

text: Generic

text: Olfactory tissue

text: Ictalurus punctatus

text: NotI

text: SalI

text: Olfactory tissue cDNA library

text: Phagemid


text: Auburn University

text: live

text: Generic

text: Trunk kidney

text: Ictalurus punctatus

text: NotI

text: SalI

text: Trunk kidney cDNA library

text: Phagemid


text: University of Minnesota

text: live

text: Generic

text: Jejunal Peyer's patches were isolated from healthy, 4-6 week old cross-bred pigs. RNA was extracted after 3 hours stimulation in an Ussing chamber with one of the following treatments 1) Salmonella choleraesuis vaccine strain SC-54, 2) phorbol myristate acetate, concanavalin A, and 8-bromo-cyclic AMP, or 3) lipopolysaccharide and cholera toxin. Each treatment was performed in the presence and absence of cycloheximide. Purified poly A + RNA from each of the treated tissues was combined together, reverse transcribed, and cloned into pCMVSPORT6 to make a library of approximately 530,000 recombinant clones with an average insert size of 1.0 kbp.

text: small intestine, jejunum

text: Sus scrofa

text: NotI

text: SalI

text: Peyer's patch

characteristic: tissue-type

text: plasmid


text: University of Minnesota

text: live

text: Generic

text: Jejunal Peyer's patches were isolated from healthy, 4-6 week old cross-bred pigs. RNA was extracted either immediately or after 3 hours in an Ussing chamber in the presence or absence of cycloheximide. Purified poly A + RNA from each of the tissues was combined together, reverse transcribed, and cloned into pCMVSPORT6 to make a library of approximately 900,000 clones with an average insert size of 1.5 kbp.

text: small intestine, jejunum

text: Sus scrofa

text: NotI

text: SalI

text: Peyer's patch

text: plasmid


text: University of Wales Aberystwyth,Institute of Biological Sciences

text: live

text: Generic

text: Three-week-old Brachypodium distachyon plants (accession ABR5) were infected with pathogen (isolate GUY11 of Magnaporthe grisea, which is a Oryzae sativa isolate). RNA was prepared from infected above ground parts harvested from 3-week-old plants 18,48,72,96 and 144 hr after inoculation. Note: accession ABR5 exhibits a resistance response to isolate GUY11. The library was made from poly-A RNA in the cloning vector lambda ZAP II. The cDNA library was differentially screened with 32P labelled first stand cDNA derived from control 0.2% gelatin treated (144 hr) plants and pooled RNAs used to construct the library. WARNING: While most or all ESTs are expected to derive from the host plant, no effort was made to eliminate ESTs deriving from the pathogen.

text: 18,48,72,96 and 144 hr after infection

text: Above ground parts of M.grisea infected plants

text: Brachypodium distachyon

text: XhoI

text: EcoRI

text: Pathogen induced ABR5/GUY11

text: pBluescript II from lambda Zap II


text: University of Nevada

text: live

text: Generic

text: Cabernet Sauvignon

text: A cDNA library from immature inflorescences at stage 12 of the modified E-L system. Tissue collected from field grown plants. A description of the modified E-L system can be found in the paper by B. G. Coombe 'Adoption of a system for identifying grapevine growth stages' (1995) Aust. J. Grape and Wine Res. 1: 104-110.

text: 12 - modified E-L system

text: Inflorescence including flowers

text: Vitis vinifera

text: Hermaphrodite

characteristic: sex

text: CabSau Flower Stage 12 (FLOu0012)

text: Plasmid

text: pZL


text: CSIRO

text: live

text: Generic

text: Cabernet Sauvignon

text: A cDNA library from immature inflorescences at stage 17 of the modified E-L system. Tissue collected from field grown plants. A description of the modified E-L system can be found in the paper by B. G. Coombe 'Adoption of a system for identifying grapevine growth stages' (1995) Aust. J. Grape and Wine Res. 1: 104-110.

text: 17 - modified E-L system

text: Inflorescence including flowers

text: Vitis vinifera

text: Hermaphrodite

text: CabSau Flower Stage 17 (FLOu0017)

text: Plasmid

text: pZL


text: CSIRO

text: live

text: Generic

text: Cabernet Sauvignon

text: A cDNA library from postveraison fruit at stage 36 of the modified E-L system. Tissue collected from field grown plants. A description of the modified E-L system can be found in the paper by B. G. Coombe 'Adoption of a system for identifying grapevine growth stages' (1995) Aust. J. Grape and Wine Res. 1: 104-110.

text: 36 - modified E-L system

text: Fruit with seeds removed

text: Vitis vinifera

text: Hermaphrodite

text: CabSau Berry Postveraison Stage 36 (POSu0036)

text: Plasmid

text: pZL


text: CSIRO

text: live

text: Generic

text: Cabernet Sauvignon

text: A cDNA library from immature inflorescences at stage 7 of the modified E-L system. Tissue collected from field grown plants. A description of the modified E-L system can be found in the paper by B. G. Coombe 'Adoption of a system for identifying grapevine growth stages' (1995) Aust. J. Grape and Wine Res. 1: 104-110.

text: 7 - modified E-L system

text: Inflorescence including flowers

text: Vitis vinifera

text: Hermaphrodite

text: CabSau Flower Stage 7 (FLOu0007)

text: Plasmid

text: pZL


text: CSIRO

text: live

text: Generic

text: Cabernet Sauvignon

text: Normalised cDNA library from immature inflorescences at stage 12 of the modified E-L system. Tissue collected from field grown plants. A description of the modified E-L system can be found in the paper by B. G. Coombe 'Adoption of a system for identifying grapevine growth stages' (1995) Aust. J. Grape and Wine Res. 1: 104-110.

text: 12 - modified E-L system

text: Inflorescence including flowers

text: Vitis vinifera

text: Hermaphrodite

text: CabSau Normalised Flower Stage 12 (FLOn0012)

text: Plasmid

text: pZL


text: CSIRO

text: live

text: Generic

text: Cabernet Sauvignon

text: A cDNA library from preveraison fruit at stage 32 of the modified E-L system. Tissue collected from field grown plants. A description of the modified E-L system can be found in the paper by B. G. Coombe 'Adoption of a system for identifying grapevine growth stages' (1995) Aust. J. Grape and Wine Res. 1: 104-110.

text: 32 - modified E-L system

text: Fruit with seeds removed

text: Vitis vinifera

text: Hermaphrodite

text: CabSau Berry Preveraison Stage 32 (PREu0032)

text: Plasmid

text: pZL


text: CSIRO

text: live

text: Generic

text: Cabernet Sauvignon

text: A cDNA library from veraison fruit at stage 35 of the modified E-L system. Tissue collected from field grown plants. A description of the modified E-L system can be found in the paper by B. G. Coombe 'Adoption of a system for identifying grapevine growth stages' (1995) Aust. J. Grape and Wine Res. 1: 104-110.

text: 35 - modified E-L system

text: Fruit with seeds removed

text: Vitis vinifera

text: Hermaphrodite

text: CabSau Berry Veraison Stage 35 (VERu0035)

text: Plasmid


text: University of Nevada

text: live

text: Generic

text: Cabernet Sauvignon

text: 36 - modified E-L system

text: Fruit with seeds removed

text: Vitis vinifera

text: Hermaphrodite

text: CabSau Normalised Berry Postveraison Stage 36 (POSn0036)

text: Plasmid

text: pZL


text: National Institute of Allergy and Infectious Diseases

text: live

text: Generic

text: secretory

characteristic: cell type

text: PCR-based cDNA library made with SMART cDNA library construction kit from Clontech

text: adult

text: salivary gland

text: Aedes aegypti

text: female

text: Liverpool / black eye

characteristic: strain

text: salivary gland

text: adult female salivary gland library

text: Phage

text: Lambda TriplEx2


characteristic: External Id

characteristic: INSDC center name

text: live

text: Generic

text: secretory

text: adult

text: salivary gland

text: Anopheles gambiae

text: female

text: salivary gland

text: Anopheles gambiae G3 adult female salivary gland library

text: Phage

text: Lambda TriplEx2


text: National Cancer Institute / NIH

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Library is oligo-dT primed and directionally cloned (EcoRV site is destroyed upon cloning). Average insert size 1.7. Library was constructed by Invitrogen.

text: DH10B-TonA (T1 and T5 phage resistant)

characteristic: lab-host

text: Pooled muscle (cardiac and skeletal)

text: Homo sapiens

text: EcoRV (destroyed)

text: NotI

text: plasmid (ampicillin resistant)


text: National Cancer Institute / NIH

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: The organ of Corti (OC) was fine dissected from a total of 386 OC as follows: 102 samples from post-natal (P) day 5; 72 from P6; 60 from P7; 46 from P8; 18 from P9; 20 from P10; 14 from P12 and 24 from P13. Total RNA was extracted using the micro Fasttrack kit (catalog # K1593-02; Invitrogen, Carlsbad, CA), according to manufacturer's instructions. Reverse transcription and library construction were carried out with the Uni-Zap XR vector kit (catalog # 237211, Stratagene) and Uni-Zap XR Gigapack III Gold Cloning kit (catalog # 237612), both from Stratagene (La Jolla, CA, USA), according to manufacturer's instructions. The frequency distribution of the library is as follows: 72% of genes have 1 copy; 14.3% 2; 12% 3-10; 1.4% 11-50 and 0.1% 51-150. As to gene function, 45% of genes are present in GenBank and have know function; 23% have hits in GenBank,but do not have assigned function; 12% are uncharacterized ESTs and 20% are unidentified. Library created in the laboratory of M. Brownstein (NIMH, NIH). A complete library decription can be found at .

text: DH10B (phage-resistant)

text: Organ of Corti/Ear

text: Mus musculus

text: EcoRI

text: XhoI

text: Organ of Corti

text: plasmid (ampicillin resistant)

text: pBluescriptSK-


text: Huazhong Agricultural University of China

text: live

text: Generic

characteristic: cultivar

text: mRNA was isolated from early bud (1-2cM), seedling with three leaves, seedling with three leaves treated by water-logging (including roots), seedling with six leaves, stem, anther from differently developing stages, immature seeds after pollinating 5 days and mature leaf samples. Individual libraries were made according to the SuperScript cDNA Synthesis and Plasmid Cloning Kit (Invitrogen). Normalized cDNA library was made by saturation hybridization with genomic DNA.

characteristic: description

text: multiple stages

characteristic: dev-stage

text: shooting root

text: Zea mays

text: hermaphrodite

text: multiple tissues

text: Normalized Multiple Tissues and Developmental Stages cDNA Library of Maize Inbred MO17

text: Plasmid


text: The First Hospital, Nanhua University

text: live

text: Generic

text: Using the technique of suppression subtractive hybridization (SSH) to construct a subtractive library containing differentially expressed genes fragments between thyroid of Graves disease tissue and normal thyroid tissue, With PCR-Select #8482; cDNA Subtraction Kit( Clontech ).

text: Adult

text: thyroid gland

characteristic: organ

text: Homo sapiens

characteristic: organism

text: EcoRI

text: female

text: Chinese

text: Graves disease

text: Human thyroid of Graves disease Subtractive Library

characteristic: title

text: plasmid

text: pUCm-T


text: Istituto Agrario di San Michele all'Adige (IASMA)

text: live

text: Generic

text: Regent

text: young inflorescence before flowering

text: inflorescence

text: Vitis vinifera

text: SalI

text: NotI

text: Hermaphrodite

characteristic: sex

text: Grape Inflorescence pSPORT1 Library

text: plasmid

characteristic: vector


text: CSIRO Livestock Industries

text: live

text: Generic

text: Library made from skeletal muscle of a 14 month old Angus steer.

text: Young Adult

text: skeletal muscle

text: Bos taurus

text: EcoRI

text: Xho I

text: male

text: Angus

characteristic: strain

text: Longissimus dorsi muscle

characteristic: tissue-type

text: Bos taurus muscle cDNA library

text: Uni-ZAPXR


characteristic: External Id

text: International Livestock Research Institute

characteristic: INSDC center name

text: live

text: Generic

text: adult day 3 feeding

text: Salivary gland

text: Rhipicephalus appendiculatus

text: Female

text: RaSG

text: plasmid

text: peptide signal trap (PST)


text: University of Bonn

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: cDNA fragments obtained with heterologous primers were cloned into pGEM

text: whole embryo

text: Bos taurus

text: Bos taurus Fragment amplification with heterologous primers


text: National Research Council Canada

text: live

characteristic: INSDC status

text: Generic

text: kidney, spleen, and liver

text: Hippoglossus hippoglossus

text: Atlantic halibut kidney, spleen, liver cDNA library

text: Lamda ZAP Express, pBK-CMV


text: live

text: Generic

characteristic: NCBI submission model

text: The oligo(dT) primed library was constructed by Stratagene in the Uni-ZAP XR vector. A mass in vivo excision of the pBluescript phagemid was performed according to manufacturer's instructions.

text: adult day 4 feeding

text: Salivary gland

text: Rhipicephalus appendiculatus

text: 90% female and 10% male

text: Ra Uni-ZAP

text: phagemid

text: pBluescript Uni-ZAP XR phagemid


text: Faculty of Biological Sciences, Catholic University of Chile

text: live

text: Generic

characteristic: NCBI submission package

text: Chardonnay

text: From leaves total RNA were isolated as described in Plant Mol Biol Reporter 11 (1993) 212-215. From berries total RNA were isolated using CTAB method and lithium chloride precipitation (Plant Mol Biol Reporter 11 (1993) 117-121 ). Double stranded cDNA were prepared from 5 ug of polyA leaf RNA or 25 ug total RNA berry RNA using a Stratagene cDNA synthesis kit. This methods produces hemymethilated cDNAs with 5'EcoRI and 3'XhoI restriction sites. These cDNA were cloning in pBluescript SK II+. Following ligation, plasmids were transformed into XL1-Blue-MRF'cells (Stratagene) and plated for blue-white selection .

text: leaf and berry

text: Vitis vinifera

text: 5' Eco R I

text: 3' Xho I

text: grape selected express library

text: phagemid

text: pBluescript II SK+


text: National Cancer Institute / NIH

text: live

text: Generic

text: DH10B TonA

characteristic: lab-host

text: embryonic stem cell

text: Homo sapiens

text: scI

text: Pac

text: Embryonic stem cell, pooled H7 ES cell line, passage 22

text: plasmid

text: pCI-neo (updated)


text: National Cancer Institute / NIH

text: live

text: Generic

text: DH10B TonA

text: embryonic stem cell

text: Homo sapiens

text: scI

text: Pac

text: Embryonic stem cells pooled from H7 ES cell line, passage 22

text: plasmid

text: pCI-neo (updated)


text: The Natural History Museum

text: live

text: Generic

text: mRNA, extracted from uninfected 1742 (susceptible) snails was used as a template for RT-PCR with random primers to generate cDNA fragments. These were cloned and sequenced using M13F. Primer sequences were removed from sequence.

characteristic: description

text: Haemopoietic organ

text: Biomphalaria glabrata

text: Hermaphrodite

text: NHM 1742

text: BgORESTES uninfected NHM 1742 Haemopoietic organ

text: pGEM


text: The Natural History Museum

text: live

text: Generic

text: mRNA, extracted from infected 1742 (susceptible) snails was used as a template for RT-PCR with random primers to generate cDNA fragments. These were cloned and sequenced using M13F. Primer sequences were removed from sequence.

text: Haemopoietic organ

text: Biomphalaria glabrata

text: Hermaphrodite

text: NHM 1742

text: BgORESTES infected NHM 1742 Haemopoietic organ

text: pGEM


text: Concordia University, Department of Biology

text: live

text: Generic

text: Conditions for growth: Seeds were germinated in a water-saturated mix (50% black earth and 50% ProMix) in a growth chamber for 7 days under an irradiance of 200 mmol m-2 sec-1. The temperature was maintained at 20 degrees C with a 15-hr photoperiod under a relative humidity of 70%. After this period watering of plants was stopped. Four time points were sampled during a two week period; the first after wilting was observed and the last, two weeks later, consisted of live crown and leaf tissue (leaf tissue that was yellow was not included in sampled material).First strand synthesis in this library was done in the presence of methylated dCTP thereby protecting from internal cleavage with NotI.

text: Crown and leaf

text: Triticum aestivum

text: Triticum aestivum FGAS: Library 4 Gate 8


text: live

text: Generic

text: Crown (50%) and leaf (50%) tissues from wheat cultivar Norstar after short exposure times to low temperature in the light and in the dark. 12 mRNA populations were combined before constructing the library. The first 6 populations: After 7 days of growth at 20Cs from wheat cultivar Norstar after short exposure times to low temperature in the light and in the dark. 12 mRNA populations were combined before constructing the library. The first 6 populations: After 7 days of growth at 20, wheat plants were transferred to 4C in the light. 1cm crown sections and green leaf tissu were separately harvested after 1, 3, and 6 hours of low temperature exposure. The last 6 populations: After 7 days of growth at 20C, wheat plants were transferred to 4C in the dark. 1cm crown sections and green leaf tissu were separately harvested after 1, 3, and 6 hours of low temperature exposure. First strand synthesis in this library was done in the presence of methylated dCTP thereby protecting from internal cleavage with NotI. In addition, this library used a primer for second strand synthesis that annealed to an artificial sequence (RNA oligo) added before first strand synthesis. Therefore when sequences from EST generated from this library will be masked for vector and adaptor sequences, an additional masking step will have to be included to mask this RNA oligo that is common to all clones (sequence CGACTGGAGCACGAGGACACTGACATGGACTGAAGGAGTAGAAA).

text: Crown and leaf

text: Triticum aestivum

text: Triticum aestivum FGAS: Library 6 CAP GATE 1


text: Ghent University

text: live

text: Generic

text: stem nodules

text: Sesbania rostrata

text: Sesbania rostrata stem nodules


text: Beijing University of Traditional Chinese Medicine

text: live

text: Generic

text: All the 6 mice are from the same mother and were born at the same time. 4 mice were kindled with PTZ(Pentylenetetrazol) at about 1 month old, inject with 32mg/kg into abdominal once day, keep for 4 weeks, stopped for 2 weeks, then kindled with the 32mg/kg. Differentially expressed genes in the kindled mouse brain were screened using an reverse transcription-polymerase chain reaction (RT-PCR) differential display (DD) method. Hippocampus RNA was isolated for DD. About 71 bands were displayed differentially. Northern blot analysis confirmed that 11 of the 71 differentially expressed chronic kindling. Further sequence analysis revealed 4 of 11 were novel.

text: hippocampus

text: Mus musculus

text: male

text: male mouse hippocampus


text: University of New Mexico

text: live

text: Generic

text: mRNA was harvested from rediae, cercariae, metacercariae, adults and 48-hour cultivated sporocysts of E. paraensei. cDNA was generated using oligo-dT primers. The sporocyst cDNA was subtracted against the combined non-sporocyst cDNA using the PCR-Select Suppressive Subtraction Hybridization kit (SSH) (Clontech, Palo Alto, CA). The resulting library was cloned into pCR2.1 (Invitrogen, Carlsbad, CA) and sequenced on both strands using plasmid-specific primers (M13 rev and M13 -20).

text: Biomphalaria glabrata

text: whole body, sporocyst

text: Echinostoma paraensei

text: EcoRI

text: EcoRI

text: E. paraensei sporocyst SSH library

text: plasmid


text: Osmania University

text: live

text: Generic

text: ICP 8744

characteristic: cultivar

text: pigeonpea plants of 4 week old were subjected to 20% Polyethylene glycol stress for 1 hour and isolated RNA from leaf and root tissues. Subtracted cDNA library was made using PCR select cDNA subtraction kit of Clontech.

text: four week old plants

characteristic: dev-stage

text: Escherichia coli

text: root and leaf tissue

text: Cajanus cajan

text: kpn I

text: apa I

text: Hermophrodite

text: root and leaf tissue

text: Pigeonpea stress induced cDNA library

text: Plasmid

text: pTNOT


text: SungKyunKwan University

text: live

text: Generic

text: Posterior silk gland

characteristic: organ

text: Bombyx mori

characteristic: organism

text: Posterior silk gland


text: Universite Laval

text: live

text: Generic

text: cDNA was prepared from 5 mg of poly A+ selected RNA and was directionally ligated into the pBluescript II SK (+) XR vector (Stratagene), transformed by electroporation into DH10B cells (In vitrogen) for propagation

text: Three stages of preformed male strobili at end of winter dormancy were pooled: swollen fully closed buds, partly open buds and fully open buds

text: E. coli DH10B cells

text: Expanding male strobili

text: Picea glauca

text: Eco-RI

text: Xho-I

text: Hermaphrodite

characteristic: sex

text: Tree 13-271

text: Entire strobilus

text: GQ001: Male strobili developmental sequence

characteristic: title

text: Plasmid

text: pBluescript II SK (+) XR


text: Universite Laval

text: live

text: Generic

text: Three stages of preformed female cones at end of winter dormancy were pooled: swollen fully closed buds, partly open buds and fully open buds

text: Expanding female cones

text: Picea glauca

text: Eco-RI

text: Xho-I

text: Hermaphrodite

text: Tree 13-271

characteristic: strain

text: Entire cone

text: GQ002: Female cones developmental sequence

text: Plasmid

text: pBluescript II SK (+) XR

characteristic: vector


characteristic: External Id

text: Universite Laval

text: live

text: Generic

text: Three stages of preformed vegetative buds at end of winter dormancy were pooled: swollen fully closed buds, partly open buds and fully open buds

text: E. coli DH10B cells

text: Expanding whole vegatative buds

text: Picea glauca

text: Eco-RI

text: Xho-I

text: Hermaphrodite

text: Tree 13-271

text: Needles and primary shoot

characteristic: tissue-type

text: GQ003: Vegetative buds developmental sequence

text: Plasmid

text: pBluescript II SK (+) XR


text: Universite Laval

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: Tissue was harvested in mid-June, during formation of early wood. cDNA was prepared from 5 mg of poly A+ selected RNA and was directionally ligated into the pBluescript II SK (+) XR vector (Stratagene), transformed by electroporation into DH10B cells (In vitrogen) for propagation

text: Non-lignified secondary xylem from trees harvested 2.5 hours, 6 hours and 11 hours after day break

text: E. coli DH10B cells

text: Stem from ground to lower part of live crown, on 33 year old tree

text: Picea glauca

text: Eco-RI

text: Xho-I

text: Hermaphrodite

text: Two trees of provenance 5333 and one from 5206

text: Non-lignified differentiating secondary xylem from normal vertical trees

text: GQ004: Non-lignified secondary xylem from mature trees

text: Plasmid

text: pBluescript II SK (+) XR


text: Universite Laval

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Cambium and phloem tissue scraped from inside of bark from trees harvested 2.5 hours, 6 hours and 11 hours after day break were pooled

text: E. coli DH10B cells

text: Picea glauca

text: Eco-RI

text: Xho-I

text: Hermaphrodite

text: Two trees of provenance 5333 and one from 5208

text: Cambium and phloem region from normal vertical trees

text: GQ006: Cambium and phloem from mature trees

text: Plasmid

text: pBluescript II SK (+) XR


text: Universite Laval

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Tissues were pooled from above and below the girdle. cDNA was prepared from 5 mg of poly A+ selected RNA and was directionally ligated into the pBluescript II SK (+) XR vector (Stratagene), transformed by electroporation into DH10B cells (In vitrogen) for propagation

text: Secondary xylem at all stages of differentiation from current and previous seasons' growth, from trees harvested 1 and 7 days after girdling treatment

text: E. coli DH10B cells

text: Main stem of 4 year old saplings, approximately 60 cm tall

text: Picea glauca

text: Eco-RI

text: Xho-I

text: Hermaphrodite

text: Secondary xylem tissues of trees girdled by removing a ring of bark ca. 1 cm wide at the midpoint of the main stem

text: GQ007: Secondary xylem of girdled saplings

text: Plasmid

text: pBluescript II SK (+) XR


text: Universite Laval

text: live

text: Generic

characteristic: description

text: Vascular cambium, secondary phloem, and bark from trees harvested 1 and 7 days after girdling treatment

text: E. coli DH10B cells

characteristic: lab-host

text: Picea glauca

text: Eco-RI

text: Xho-I

text: Hermaphrodite

text: Vascular cambium, secondary phloem and bark of trees girdled by removing a ring of bark ca. 1 cm wide from the midpoint of the main stem

text: GQ008: Cambium, phloem and bark of girdled saplings

text: Plasmid

text: pBluescript II SK (+) XR


text: Lawrence Berkeley National Lab

text: live

text: Generic

text: The stem cell enriched testis library was made from adult males overexpressing a UAS-unpaired transgene in germ cells from the nos-vp16 Gal4 driver. The UAS-unpaired transgenic flies were in a y,w genetic background and the nos-vp16 Gal4 driver were in a w background. Additionally there was a UAS-gfp transgene recombined onto the nosGAL4 chromosome. RNA was isolated from hand dissected testes from 0-3 day old males. RNA kindly provided by the lab of Dr.Margaret Fuller.

text: 0-3 day old males

characteristic: dev-stage

text: DH5-alpha TonA

text: ADULT testes

text: Drosophila melanogaster

text: EcoRI

text: male

text: UT Drosophila melanogaster adult testes pOTB7


text: The Natural History Museum

text: live

text: Generic

text: mRNA, extracted from schistosome-unexposed 3017 (resistant) snails was used as a template for RT-PCR with random primers to generate cDNA fragments. These were cloned and sequenced using M13F. Primer sequences were removed from sequence.

text: Haemopoietic organ

characteristic: organ

text: Biomphalaria glabrata

text: Hermaphrodite

text: NHM 3017

text: BgORESTES unexposed NHM 3017 Haemopoietic organ

text: pGEM


text: The Natural History Museum

text: live

text: Generic

text: mRNA, extracted from schistosome-exposed 3017 (resistant) snails was used as a template for RT-PCR with random primers to generate cDNA fragments. These were cloned and sequenced using M13F. Primer sequences were removed from sequence.

text: Haemopoietic organ

text: Biomphalaria glabrata

characteristic: organism

text: Hermaphrodite

text: NHM 3017

text: BgORESTES schistosome-exposed NHM 3017 Haemopoietic organ

text: pGEM


text: Southwest Agricultural University

text: live

text: Generic

text: Ovary of 2-3 cm in length and 2 days before flowering, young fruit of 0, 6, 12, 24, 48, 72 hours after pollination and 3 days without pollination after flowering

text: Young fruit before and after pollination within 4 days

text: Young fruit

text: Cucumis sativus

text: Female (gynoecious)

characteristic: sex

text: Cucumber Young Fruit cDNA Lambda TriplEx Library

text: Lambda TriplEx2 vector


text: Huazhong Agricultural University of China

text: live

text: Generic

characteristic: cultivar

text: mRNA was isolated from endosperm 4 days after pollinating and 8 DAP and 14 DAP and 18 DAP and 26 DAP and 40 DAP. Individual libraries were made according to the SuperScript cDNA Synthesis and Plasmid Cloning Kit (Invitrogen). Normalized cDNA library was made by saturation hybridization with genomic DNA.

text: multiple stages

text: single organs

text: Zea mays

text: hermaphrodite

text: single tissues

text: Normalized endosperm cDNA Library of Maize hybrid YUYU22

characteristic: title

text: Plasmid


text: University Victor Segalen Bordeaux 2 (FRANCE)

text: live

text: Generic

text: Cochlear and vestibular cDNA libraries were built from P10 mice. A subtractive hybridization was realized according to the CLONTECH PCR-Select cDNA subtraction kit protocol(cat# K1804-1). The amplified sequences were then cloned into PROMEGA pGEMT vector. Three 96 wells plates were sequenced and analysed.

text: 10 days post natal

text: Cochleae and vestibuli (inner ear)

text: Mus musculus

text: male

characteristic: strain

text: Cochlear and vestibular tissues cDNA subtractive hybridization

text: plasmid

text: pGEMT (PROMEGA)

characteristic: vector


characteristic: External Id

text: Instituto de Ciencias Biomedicas

text: live

text: Generic

text: 4th instar 3rd period

text: Salivary Glands

text: Rhynchosciara americana

text: NotI

text: EcoRI

text: Salivary Gland 4th instar 3rd period (spinning cocoon)

text: Plasmid


text: Michigan State University Department of Horticulture/Washington Tree Fruit Research Commission/Michigan Agricultural Experiment Station

characteristic: INSDC center name

text: live

text: Generic

text: Mutsu

text: various

text: whole fruit

text: Malus domestica

text: apple (Malus x domestica) fruit


text: CSIRO

characteristic: INSDC first public

text: live

text: Generic

text: Cabernet Sauvignon

text: A cDNA library from postveraison fruit at stage 38 of the modified E-L system. Tissue collected from field grown plants. A description of the modified E-L system can be found in the paper by B. G. Coombe 'Adoption of a system for identifying grapevine growth stages' (1995) Aust. J. Grape and Wine Res. 1: 104-110.

text: 38 - modified E-L system

text: Fruit with seeds removed

text: Vitis vinifera

text: Hermaphrodite

text: CabSau Berry Postveraison Stage 38 (POSu0038)

text: Plasmid


text: Animal and Natural Resources Institute

characteristic: INSDC last update

text: live

text: Generic

text: Equimolar amounts of mRNA extracted from proximal jejunums of 18 and 21 wk old steers, and distal ileums of 14 day old calves. proximal jejunum exposed to C. oncophora for 3 and 6 weeks, and distal ileum exposed to C. parvum for 7 days

text: Multiple

text: DH10B T1 phage resistant

text: Small Intestine

text: Bos taurus

text: EcoRV

text: NotI

text: Male

text: Holstein

text: Pooled

characteristic: tissue-type

text: BARC 10BOV

text: plasmid


text: University of Nevada

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Cabernet Sauvignon

text: A cDNA library from nectaries dissected from flowers at stage 25 of the modified E-L system. Tissue collected from field grown plants. A description of the modified E-L system can be found in the paper by B. G. Coombe 'Adoption of a system for identifying grapevine growth stages' (1995) Aust. J. Grape and Wine Res. 1: 104-110.

text: 25 - modified E-L system

text: Nectary of flowers

text: Vitis vinifera

text: Hermaphrodite

text: CabSau Flower Nectary Stage 25 (NECu0025)

text: Plasmid


text: National Cancer Institute / NIH

text: live

text: Generic

characteristic: NCBI submission package

text: 1st strand cDNA was primed with an oligo(dT) primer [GCGGCTGAAGACGGCCTATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded cDNA was ligated to a DraIII adaptor [GGCCUACUGG], digested and directionally cloned into distinct DraIII sites of the pME18S-FL3. Library was size selected for 1.0 kb, with a average insert size of ~1.2kb. Library constructed by Yutaka Suzuki (University of Tokyo Institute of Medical Science). Custom primers recommended for sequencing: 5' end primer 5'-GGATGTTGCCTTTACTTCTA-3' and 3' end primer 5'-CGACCTGCAGCTCGAGCACA-3'. Note: This is a Zebrafish Gene Collection (ZGC) library

characteristic: description

text: DH10B TonA

text: olfactory epithelium

text: Danio rerio

text: DraIII

text: DraIII

text: olfactory epithelium

text: phagemid


text: Oklahoma State University

text: live

text: Generic

text: Differential display PCR products cloned in pDrive

text: adult

characteristic: dev-stage

text: sheep

characteristic: lab-host

text: salivary gland

text: Dermacentor andersoni

text: male

text: Male Dermacentor andersoni effect of feeding

text: plasmid

text: pDrive


text: The University of Georgia, Department of Plant Biology

text: live

text: Generic

text: The library was prepared from polyA+ RNA from oxidatively stressed, hydroponically grown sorghum seedlings. At 8 days of age, growth medium was supplemented with hydrogen peroxide to 0.003% and leaves were misted with 10 uM methyl viologen. Leaves and roots were harvested at 3, 12 and 27 hr after treatment and all tissue pooled. Double-stranded cDNA was cloned unidirectionally into different DraIII sites of the pME18S-FL3 vector (5-prime DraIII site is CACTGTGTG, 3-prime DraIII site is CACCATGTG). XhoI excises the cDNA insert.

text: DH10B-T1 phage-resistant E. coli

text: Leaf and Root

characteristic: organ

text: Sorghum bicolor

characteristic: organism

text: XhoI

text: XhoI

text: Oxidatively-stressed leaves and roots

text: Plasmid


text: University of British Columbia

text: live

text: Generic

characteristic: cultivar

text: Bark was wounded using razor blades along the entire length of the tree at 5 mm intervals on opposite sides of the trunk. The same trees were also sprayed with a 0.01% (v/v) methyl jasmonate solution resuspended in 0.1% (v/v) tween 20 (~50mLs per tree). Bark tissue with phloem attached was harvested 24 hours, 2 days, 4 days and 8 days after initiating the treatment. mRNA was isolated from each tissue source independently and equal quantities of mRNA from each tissue were then pooled. cDNA was prepared from 5 micrograms of mRNA and directionally ligated into the pBluescript II SK (+) XR vector using the pBluescript II XR cDNA Library Construction Kit according to manufacturer's instructions with modifications (Stratagene). Plasmid DNA was then transformed by electroporation into DH10B cells (Invitrogen) for propagation.

text: E. coli DH10B cells

text: Bark (with phloem and cambium attached) from one year old clonal trees grown under greenhouse conditions in standard potting soil mixture

text: Picea engelmannii x Picea glauca

text: EcoRI (5' end of cDNA)

text: XhoI (3' end of cDNA)

text: Hermaphrodite

characteristic: sex

text: pBluescript II SK (+) XR


text: University of British Columbia

text: live

text: Generic

text: Bark was wounded using razor blades along the entire length of the tree at 5 mm intervals on opposite sides of the trunk. The same trees were also sprayed with a 0.01% (v/v) methyl jasmonate solution resuspended in 0.1% (v/v) tween 20 (~50mLs per tree). Bark tissue with phloem attached was harvested 3 hours, 6 hours, 12 hours, 24 hours, 2 days, 4 days and 8 days after initiating the treatment. Untreated control bark was also harvested at time 0 hours. mRNA was isolated from each tissue source independently and equal quantities of mRNA from each tissue were then pooled. cDNA was prepared from 5 micrograms of mRNA and directionally ligated into the pBluescript II SK (+) XR vector using the pBluescript II XR cDNA Library Construction Kit according to manufacturer's instructions with modifications (Stratagene). Plasmid DNA was then transformed by electroporation into DH10B cells (Invitrogen) for propagation. Normalization was applied according to published methods [Bonaldo M.F. et al. (1996) Genome Research 6(9):791] in order to reduce the abundance of highly expressed transcripts.

text: E. coli DH10B cells

text: Picea engelmannii x Picea glauca

text: EcoRI (5' end of cDNA)

text: XhoI (3' end of cDNA)

text: Hermaphrodite

characteristic: title

text: pBluescript II SK (+) XR


text: University of British Columbia

text: live

text: Generic

text: mRNA was isolated from each tissue source independently and equal quantities of mRNA from each tissue were then pooled. cDNA was prepared from 5 micrograms of mRNA and directionally ligated into the pBluescript II SK (+) XR vector using the pBluescript II XR cDNA Library Construction Kit according to manufacturer's instructions with modifications (Stratagene). Plasmid DNA was then transformed by electroporation into DH10B cells (Invitrogen) for propagation.

text: E. coli DH10B cells

text: Roots from three year old clonal trees grown under greenhouse conditions in standard potting soil mixture

text: Picea sitchensis

text: EcoRI (5' end of cDNA)

text: XhoI (3' end of cDNA)

text: Hermaphrodite

text: Young root growth (terminal 1-3 cm) and old root growth (distal to terminal 1-3 cm) tissues were harvested

text: pBluescript II SK (+) XR

characteristic: vector


characteristic: External Id

text: University of British Columbia

text: live

text: Generic

text: cDNA was prepared from 5 micrograms of mRNA and directionally ligated into the pBluescript II SK (+) XR vector using the pBluescript II XR cDNA Library Construction Kit according to manufacturer's instructions with modifications (Stratagene). Plasmid DNA was then transformed by electroporation into DH10B cells (Invitrogen) for propagation.

text: E. coli DH10B cells

text: Foliage from 25 year old trees harvested at Kalamalka Research Station in Vernon, British Columbia on June 15th, 2001

text: Picea glauca

text: EcoRI (5' end of cDNA)

text: XhoI (3' end of cDNA)

text: Hermaphrodite

text: Young shoots

text: pBluescript II SK (+) XR


text: University of British Columbia

characteristic: INSDC center name

text: live

text: Generic

text: E. coli DH10B cells

text: Phloem from 25 year old trees harvested at Kalamalka Research Station in Vernon, British Columbia in 2001

text: Picea glauca

text: EcoRI (5' end of cDNA)

text: XhoI (3' end of cDNA)

text: Hermaphrodite

text: Early season phloem harvested June 15th, mid season phloem harvested July 10th and late season phloem harvested August 17th

text: pBluescript II SK (+) XR


text: University of British Columbia

characteristic: INSDC first public

text: live

text: Generic

text: E. coli DH10B cells

text: Foliage from 25 year old trees harvested at Kalamalka Research Station in Vernon, British Columbia in 2001

text: Picea glauca

text: EcoRI (5' end of cDNA)

text: XhoI (3' end of cDNA)

text: Hermaphrodite

text: Flushing buds harvested May 16th, young shoots harvested June 15th and mature shoots harvested June 15th

text: pBluescript II SK (+) XR


text: University of British Columbia

characteristic: INSDC last update

text: live

text: Generic

text: mRNA was isolated from each tissue source independently and equal quantities of mRNA from each tissue were then pooled. cDNA was prepared from 5 micrograms of mRNA and directionally ligated into the pBluescript II SK (+) XR vector using the pBluescript II XR cDNA Library Construction Kit according to manufacturer's instructions with modifications (Stratagene). Plasmid DNA was then transformed by electroporation into DH10B cells (Invitrogen) for propagation. Normalization was applied according to published methods [Bonaldo M.F. et al. (1996) Genome Research 6(9):791] in order to reduce the abundance of highly expressed transcripts.

text: E. coli DH10B cells

text: Picea glauca

text: EcoRI (5' end of cDNA)

text: XhoI (3' end of cDNA)

text: Hermaphrodite

text: Flushing buds harvested May 16th, young shoots harvested June 15th, and mature shoots harvested June 15th

text: pBluescript II SK (+) XR


text: University of British Columbia

text: live

text: Generic

text: E. coli DH10B cells

text: Outer xylem from 25 year old trees harvested at Kalamalka Research Station in Vernon, British Columbia in 2001

text: Picea glauca

text: EcoRI (5' end of cDNA)

text: XhoI (3' end of cDNA)

text: Hermaphrodite

text: Early season xylem harvested June 15th, mid season xylem harvested July 10th and late season xylem harvested August 17th

characteristic: tissue-type

text: pBluescript II SK (+) XR


text: University of British Columbia

text: live

characteristic: INSDC status

text: Generic

text: E. coli DH10B cells

text: Picea glauca

text: EcoRI (5' end of cDNA)

text: XhoI (3' end of cDNA)

text: Hermaphrodite

text: pBluescript II SK (+) XR


text: Universite Laval

text: live

text: Generic

characteristic: NCBI submission model

text: Stems harvested from the midsection of trees subjected either to fertilization with 0 or 25 mM ammonium nitrate for 1, 3, or 7 days, or removal of a ring of bark from the main stem (stem girdling) for 1 or 6 days. cDNA was prepared from 5 N<g of poly A+ selected RNA and was directionally ligated into the pBluescript II SK (+) XR vector (Stratagene), transformed by electroporation into DH10B cells (In vitrogen) for propagation

text: E. coli DH10B cells

text: Whole secondary stems of trees approximately 80-120 cm tall

text: Populus trichocarpa x Populus deltoides

text: Eco-RI

text: Xho-I

text: MALE

characteristic: strain

text: Whole stems with secondary growth, composed of secondary xylem, secondary phloem, cambium, and bark

text: Plasmid

text: pBluescript II SK (+) XR


text: Universite Laval

text: live

text: Generic

characteristic: NCBI submission package

text: Whole stems with primary growth were harvested between LPI 1 and 4 from trees treated either with low vs. high levels of ammonium nitrate fertilization for 7 days, or with different sources of nitrogen or carbon for 4 or 24 hours. cDNA was prepared from 5 N<g of poly A+ selected RNA and was directionally ligated into the pBluescript II SK (+) XR vector (Stratagene), transformed by electroporation into DH10B cells (In vitrogen) for propagation

characteristic: description

text: E. coli DH10B cells

text: Whole primary stems of trees approximately 80-120 cm tall

text: Populus trichocarpa x Populus deltoides

text: Eco-RI

text: Xho-I

text: MALE

text: Primary xylem and primary phloem from whole stems

text: Plasmid

text: pBluescript II SK (+) XR


text: Universite Laval

text: live

text: Generic

text: Young leaves (LPI 1-3), mature leaves (LPI 7-9) and senescing leaves (LPI 15-17) from trees treated either with low vs. high levels of ammonium nitrate fertilization for 7 days, or with different sources of nitrogen or carbon for 4 or 24 hours. cDNA was prepared from 5 N<g of poly A+ selected RNA and was directionally ligated into the pBluescript II SK (+) XR vector (Stratagene), transformed by electroporation into DH10B cells (In vitrogen) for propagation

characteristic: dev-stage

text: E. coli DH10B cells

characteristic: lab-host

text: Leaves of trees approximately 80-120 cm tall

text: Populus trichocarpa x Populus deltoides

text: Eco-RI

text: Xho-I

text: MALE

text: Leaves

text: Plasmid

text: pBluescript II SK (+) XR


text: Universite Laval

text: live

text: Generic

text: Actively growing shoot tips of trees subjected to fertilization with 0 or 25 mM ammonium nitrate for 1, 3, or 7 days, or removal of a ring of bark from the main stem (stem girdling) for 1 or 6 days, or treatment with different sources of nitrogen and carbon for 4 or 24 hours. cDNA was prepared from 5 N<g of poly A+ selected RNA and was directionally ligated into the pBluescript II SK (+) XR vector (Stratagene), transformed by electroporation into DH10B cells (In vitrogen) for propagation

text: E. coli DH10B cells

text: Shoot apex, primary stem and developing leaves up to and including LPI 1

characteristic: organ

text: Populus trichocarpa x Populus deltoides

characteristic: organism

text: Eco-RI

text: Xho-I

text: MALE

text: Shoot tips of trees approximately 80-120 cm tall

text: Plasmid

text: pBluescript II SK (+) XR


text: Universite Laval

text: live

text: Generic

text: Shoot tips (including apex, primary stem and developing leaves up to and including LPI 1) and whole stems (wood and bark) undergoing secondary growth taken from between LPI 8 and LPI 15. Pooled sample of tissues harvested every two hours during one 12 hour light/12 hour dark cycle, beginning 1 hour before the beginning of the light period and ending 3 hours after the end of the light cycle. cDNA was prepared from 5 N<g of poly A+ selected RNA and was directionally ligated into the pBluescript II SK (+) XR vector (Stratagene), transformed by electroporation into DH10B cells (In vitrogen) for propagation

text: E. coli DH10B cells

text: Shoot tips and secondary stems of trees approximately 80 cm tall

text: Populus trichocarpa x Populus deltoides

text: Eco-RI

text: Xho-I

text: MALE

characteristic: sex

text: Plasmid

text: pBluescript II SK (+) XR


text: Institut National de la Recherche Agronomique

text: live

text: Generic

text: Shiraz

characteristic: cultivar

text: Oriented library, construction described in Generation of ESTs from grape Berry (skin, pulp or seeds) at various developmental stages by Terrier,N., Ageorges,A., Abbal,P., Romieu,C. in J. Plant Physiol. 158 (12): 1575-83 2001

text: green stage

text: Fruit without seeds

text: Vitis vinifera

text: Eco RI

text: XhoI

text: Green Grape Berry LambdaZapII Library

characteristic: title

text: Phage

text: LambdaZapII


characteristic: External Id

text: live

text: Generic

text: Shiraz

text: Ripe stage

text: Fruit without seeds

text: Vitis vinifera

text: Eco RI

text: XhoI

text: Ripe Grape Berry LambdaZapII Library

text: Phage

text: LambdaZapII

characteristic: vector


characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: Shiraz

text: Veraison stage

text: Fruit without seeds

text: Vitis vinifera

text: Eco RI

text: XhoI

text: Turning Grape Berry LambdaZapII Library

text: Phage

text: LambdaZapII


text: Universite de Bordeaux I, Institut National de la Recherche Agronomique

characteristic: INSDC last update

text: live

text: Generic

text: Cabernet Sauvignon

text: Oriented library

text: green stage

text: Fruit without seeds

text: Vitis vinifera

text: SfiIA

text: SfiIB

text: Green Grape Berry Lambda Triplex2 Library

text: Phage

text: Lambda TriplEx2


text: live

characteristic: INSDC status

text: Generic

text: Cabernet Sauvignon

text: Oriented library

text: ripe stage

text: Fruit without seeds

text: Vitis vinifera

text: SfiIA

text: SfiIB

text: Ripe Grape Berry Lambda Triplex2 Library

text: Phage

text: Lambda TriplEx2


text: live

text: Generic

characteristic: NCBI submission model

text: Cabernet Sauvignon

text: Oriented library

text: veraison stage

text: Fruit without seeds

text: Vitis vinifera

text: SfiIA

text: SfiIB

text: Turning Grape Berry Lambda Triplex2 Library

text: Phage

text: Lambda TriplEx2


text: Universite Laval

text: live

text: Generic

text: cDNA was prepared from 5 N<g of poly A+ selected RNA and was directionally ligated into the pBluescript II SK (+) XR vector (Stratagene), transformed by electroporation into DH10B cells (In vitrogen) for propagation

text: Secondary developing lignified and unlignified xylem, secondary developing phloem

text: E. coli DH10B cells

text: Current year's growth taken from two regions of mature tree trunk

text: Populus trichocarpa x Populus deltoides

text: Eco-RI

text: Xho-I

text: Female

text: Beaupre 3226

characteristic: strain

text: Secondary xylem, secondary phloem representing current year's growth of main stem of mature tree

characteristic: tissue-type

text: Plasmid

text: pBluescript II SK (+) XR


text: Institut de Recherche pour le Developpement (IRD)

text: live

text: Generic

characteristic: NCBI submission package

text: tenera LMC249

text: cDNA library constructed from poly(A)+ RNAs using the ZAP-cDNA synthesis kit and the ZAP-cDNA Gigapack(r) III Gold packaging extract (Stratagen).

characteristic: description

text: in vitro plants

text: 1 cm shoot apex-containing leaf segment

text: Elaeis guineensis

text: EcoRI

text: XhoI

text: Oil palm normal apex Lambda Zap Library

text: Phage

text: Lambda Zap


text: live

text: Generic

text: tenera LMC3

text: in vitro plants

characteristic: dev-stage

text: 1 cm shoot apex-containing leaf segment

text: Elaeis guineensis

text: EcoRI

text: XhoI

text: Oil palm abnormal apex Lambda Zap Library

text: Phage

text: Lambda Zap


text: live

text: Generic

text: tenera LMC17

text: 20 cm long inflorescence

text: Female inflorescence

characteristic: organ

text: Elaeis guineensis

text: EcoRI

text: XhoI

text: Oil palm female inflorescence Lambda Zap Library

text: Phage

text: Lambda Zap


text: live

text: Generic

text: tenera C1001

text: 20 cm long inflorescence

text: Male inflorescence

text: Elaeis guineensis

characteristic: organism

text: EcoRI

text: XhoI

text: Oil palm male inflorescence Lambda Zap Library

text: Phage

text: Lambda Zap


text: live

text: Generic

text: tenera C1001

text: 3-9 months old embryos

text: zygotic embryo

text: Elaeis guineensis

text: EcoRI

text: XhoI

text: Oil palm immature zygotic embryo Lambda Zap Library

text: Phage

text: Lambda Zap


text: University of Waterloo

text: live

text: Generic

text: Differential display PCR performed on copper-exposed plants. Total RNA was reverse transcribed and amplified using two 18mer arbitrary primers. Fragments between 250-600bp were ligated into pGEM T Easy and later sequenced.

text: 3 days post-germination

text: Whole Plant

text: Lemna gibba

text: PCR cDNA Library

text: pGEM T Easy


text: Huazhong agricultural university of China

text: live

text: Generic

text: mRNA was isolated from Longissimus Dorsi muscle tissue and backfat of Meishan and Large White and hybrids, and mRNA differential display was conducted to compare the gene differential expression, then differentially expressed bands were reamplfied and cloned to T-vector and sequenced, at last using semi-quantitative RT-PCR identified.

text: multiple stages

text: E.coli JM109

characteristic: lab-host

text: multiple organs

text: Sus scrofa

text: male and female

characteristic: sex

text: Meishan and Large White and hybrids

text: muscle tissue and fat tissue

text: Normalized cDNA from Longissimus Dorsi muscle and backfat tissues of pigs

text: Plasmid

text: pMD-18 T vector


text: The Institute for Genomic Research

text: live

text: Generic

text: 7-56 mother tree, open-pollinated tree from, Lyons, Georgia, USA

characteristic: cultivar

text: tissue: Whole megagametophytes isolated from pine seeds, Whole embryos excised from these megagametophytes, whole somatic embryos and suspensor tissue from tissue culture, isolated from cell line A12. Pooled RNA from zygotic embryos, megagametophytes, and somatic embryos was used for library construction. Pine cones were harvested weekly from open-pollinated 7-56 mother trees, collections occurred from 7/01/02 until 10/15/02. Whole megagametophytes were first isolated from pine seeds, and whole embryos excised from these megagametophytes each was flash frozen. Embryo development was assessed using the system of Pullman et al (Pullman GS, Johnson S, Peter G, Cairney J, Xu N. 2003. Loblolly Pine somatic embryogenesis: development of a maturation medium and resulting embryo quality. Plant Cell Reports 21:747-758 (http://link.springer.de/link/service/journals/00299/contents/03/00586/). For photographs see Ciavatta et al 2001. (Ciavatta VT, Morillon R, Pullman GS, Chrispeels M, Cairney J. 2001. An aquaglyceroporin is abundantly expressed early in the development of the suspensor and the embryo proper of loblolly pine (Pinus taeda L.). Plant Physiol. 127: 1556-1567 (http://www.plantphysiol.org/cgi/content/full/127/4/1556)).

text: E.coli DH10B-TonA

text: Zygotic Embryo and Megagametophyte, Somatic Embryo

text: Pinus taeda

text: NotI

text: EcoRV

text: Sequencing ESTs from loblolly pine embryos

characteristic: title

text: plasmid

text: pCMV-SPORT 6.1


characteristic: External Id

text: Instituto Butantan

text: live

text: Generic

text: 4ug of mRNA from Haementeria depressa salivary complexes were primed with oligo- (dT) and reverse transcribed to cDNA using Superscript Plasmid System for cDNA Synthesis and Cloning (Invitrogen). The cDNAs were selected by size (400-800 pb and up 800 pb) in agarose gel electrophoresis, linked to Eco RI adapters and directionally cloned in pGEM11Zf+ vector (Promega). ESTs were generated from random clones and grouped in unique sequences. The putative identification of each EST or cluster was obtained through Blast searches (e-value < e-05).

text: salivary complexes

text: Haementeria depressa

text: Eco RI

text: Not I

text: salivary glands

text: Leech Haementeria depressa library HDA

text: plasmid

characteristic: vector


text: Max Planck Institute of Molecular Plant Physiology

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: Gifu (B-129)

text: The library was prepared using mRNA extracted from green seed pods at very early stages to fully developed green pods.

text: Early stages to fully developed green pods

text: Seeds and seed pods

text: Lotus japonicus

text: EcoRI

text: XhoI

text: Lotus japonicus seed and seed pod library

text: Plasmid


text: Agriculture & Agri-Food Canada

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: DH12075 (double haploid line from Cresor x Westar cross)

text: Seeds were collected by Dr. Francois Ouellet when they were still very green (mid to large stage, cotyledons were formed). The seed coats were removed and the remaining tissue was used for cDNA library construction. mRNA was poly-A primed using SuperScript Plasmid System cDNA Synthesis and Cloning kit (Invitrogen) After initial screening, the most abundant redundant clones were screened out using 22 oligos designed to match napins (including albumins), cruciferins, oleosins, trypsin inhibitor 2, cytosolic GAPDH, cyclophilins, HSP70, desaturase, and CAB (LHCP).

text: Mid to late embryos (4-6 mg)

text: E. coli ElectroMAX DH5 alpha-e (Invitrogen)

text: Embryos without seed coat

text: Brassica napus

text: NotI

text: SalI

text: Brassica embryo library (EL)

text: Plasmid

text: pSPORT1 (modified: GCGGCCGCCc*GACTAGTGAGCTC*cggacgcgtggGTCGAC)


text: IRD (Institut de Recherche pour le Developpement)

text: live

text: Generic

characteristic: NCBI submission model

text: 3 weeks after inoculation by Frankia

text: Young nodules

text: Casuarina glauca

text: SfiIA

text: SfiIB

text: Casuarina glauca Nodules

text: phagemid


text: Lawrence Berkeley National Lab

text: live

text: Generic

text: Random primed, normalized library from mixed stage embryos, imaginal disks, and adult heads.

text: Drosophila melanogaster

text: NotI

text: XhoI

text: Exelixis FlyTag CK01 pCDNA-SK+

text: pCDNA-SK+


text: Lawrence Berkeley National Lab

text: live

text: Generic

text: Random primed, normalized library from mixed stage embryos, imaginal disks, and adult heads. Subset of Exelixis FlyTag CK01 clones sequenced from 3' end

text: Drosophila melanogaster

text: NotI

text: XhoI

text: Exelixis FlyTag CK02 pCDNA-SK+

text: pCDNA-SK+


text: CSIRO Plant Industry

text: live

text: Generic

text: Poly A+ mRNA cloned directionally into the pCMVSport6 vector.

text: Pre flowering

text: Whole Plant

text: Papaver somniferum

text: Tasmanian Inbred

characteristic: strain

text: Leaf

characteristic: tissue-type

text: Opium poppy leaf cDNA library


text: Temasek Lifesciences Laboratory

text: live

text: Generic

characteristic: NCBI submission package

text: Full length cDNA was synthesized from adult testis poly(A)+ RNA with ZAP-cDNA Synthesis Kit (Stratagene). Size-fractionated cDNA (mainly 0.5-3kb in length) were directionally cloned into Uni-ZAP XR vector. The cDNA clones were excised from Uni-ZAP vector using ExAssist helper phage. Excised pBluescript phagemids were used to transform E coli XL1-Blue cells. The insert from randomly selected white colonies was PCR amplified using M13 forward and reverse primers and partially sequenced by using M13 reverse primer.

characteristic: description

text: adult (fully mature)

text: pooled testis

text: Danio rerio

text: EcoRI

text: XhoI

text: male

text: AB

text: adult testis full-length (TLL)

text: pBluescript II SK


text: Temasek Lifesciences Laboratory

text: live

text: Generic

text: Full length cDNA was synthesized from adult ovary poly(A)+ RNA with ZAP-cDNA Synthesis Kit (Stratagene). Size-fractionated cDNA (mainly 0.5-3kb in length) were directionally cloned into Uni-ZAP XR vector. The cDNA clones were excised from Uni-ZAP vector using ExAssist helper phage. Excised pBluescript phagemids were used to transform E coli XL1-Blue cells. The insert from randomly selected white colonies was PCR amplified using M13 forward and reverse primers and partially sequenced by using M13 reverse primer.

text: adult (fully mature)

characteristic: dev-stage

text: pooled ovary

characteristic: organ

text: Danio rerio

text: EcoRI

text: XhoI

text: female

text: AB

text: adult ovary full-length (TLL)

text: pBluescript II SK


text: Temasek Lifesciences Laboratory

text: live

text: Generic

text: Full length cDNA was synthesized from adult testis poly(A)+ RNA with ZAP-cDNA Synthesis Kit (Stratagene). Size-fractionated cDNA (mainly 0.5-3kb in length) were directionally cloned into Uni-ZAP XR vector. The cDNA clones were excised from Uni-ZAP vector using ExAssist helper phage. Excised pBluescript phagemids were used to transform E coli XL1-Blue cells. The plasmid library was normalized by using a reassociation-kinetics-based approach according to Bonaldo et al., (Genome Research 6: 791-806,1996) with minor modifications. The insert from randomly selected white colonies was PCR amplified using M13 forward and reverse primers and partially sequenced by using M13 reverse primer.

text: adult (fully mature)

text: pooled testis

text: Danio rerio

characteristic: organism

text: EcoRI

text: XhoI

text: male

text: AB

text: adult testis normalized (TLL)

text: pBluescript II SK


text: Temasek Lifesciences Laboratory

text: live

text: Generic

text: Full length cDNA was synthesized from adult ovary poly(A)+ RNA with ZAP-cDNA Synthesis Kit (Stratagene). Size-fractionated cDNA (mainly 0.5-3kb in length) were directionally cloned into Uni-ZAP XR vector. The cDNA clones were excised from Uni-ZAP vector using ExAssist helper phage. Excised pBluescript phagemids were used to transform E coli XL1-Blue cells. The plasmid library was normalized by using a reassociation-kinetics-based approach according to Bonaldo et al., (Genome Research 6: 791-806,1996) with minor modifications. The insert from randomly selected white colonies was PCR amplified using M13 forward and reverse primers and partially sequenced by using M13 reverse primer.

text: adult (fully mature)

text: pooled ovary

text: Danio rerio

text: EcoRI

text: XhoI

text: female

characteristic: sex

text: AB

text: adult ovary normalized (TLL)

text: pBluescript II SK


text: Temasek Lifesciences Laboratory

text: live

text: Generic

text: cDNA was synthesized from adult testis and ovary total RNA using SMART PCR cDNA synthesis kit (Clontech). PCR-SelectTM cDNA subtraction kit (Clontech) was used to enrich for fragments, which were present in the testis but not in the ovary cDNA. The selectively amplified cDNA fragments (in average 400-800bp in length) were ligated into pT-Advantage (Clontech) and transformed into XL10-Gold competent cells (Stratagene). The insert from randomly selected white colonies was PCR amplified using M13 forward and reverse primers and partially sequenced by using M13 reverse primer.

text: adult (fully mature)

text: pooled testis

text: Danio rerio

text: male

text: Toh (Singaporean strain)

text: adult testis subtracted 1 (TLL)

text: pT-Advantage


text: Temasek Lifesciences Laboratory

text: live

text: Generic

text: cDNA was synthesized from adult testis and liver total RNA using SMART PCR cDNA synthesis kit (Clontech). PCR-SelectTM cDNA subtraction kit (Clontech) was used to enrich for fragments, which were present in the testis but not in the liver cDNA. The selectively amplified cDNA fragments (in average 400-800bp in length) were ligated into pGEM-T-Easy (Promega) and transformed into XL10-Gold competent cells (Stratagene). The insert from randomly selected white colonies was PCR amplified using M13 forward and reverse primers and partially sequenced by using M13 reverse primer.

text: adult (fully mature)

text: pooled testis

text: Danio rerio

text: male

text: Toh (Singaporean strain)

text: adult testis subtracted 2 (TLL)

text: pGEM-T-Easy


text: Temasek Lifesciences Laboratory

text: live

text: Generic

text: cDNA was synthesized from adult ovary and testis total RNA using SMART PCR cDNA synthesis kit (Clontech). PCR-SelectTM cDNA subtraction kit (Clontech) was used to enrich for fragments, which were present in the ovary but not in the testis cDNA. The selectively amplified cDNA fragments (in average 400-800bp in length) were ligated into pT-Advantage (Clontech) transformed into XL10-Gold competent cells (Stratagene). The insert from randomly selected white colonies was PCR amplified using M13 forward and reverse primers and partially sequenced by using M13 reverse primer.

text: adult (fully mature)

text: pooled ovary

text: Danio rerio

text: female

text: Toh (Singaporean strain)

text: adult ovary subtracted (TLL)

text: pT-Advantage


text: Temasek Lifesciences Laboratory

text: live

text: Generic

text: ORESTES libraries were generated according to Neto et al. (Gene 186: 135-142, 1997). mRNA isolated from adult testis was reverse transcribed and PCR amplified using oligonucleotide primers designed for AFLP analysis or for amplification of specific genes from Arabidopsis, yeast or rice. The amplification products with a smear or multiple bands (> 500 bp) were then cloned into pGEM-T vector (Promega) and transformed into XL-10 Gold competent cell (Stratagene). The insert from randomly selected white colonies was PCR amplified using M13 forward and reverse primers and partially sequenced by using M13 reverse primer.

text: adult (fully mature)

text: pooled testis

text: Danio rerio

text: male

text: Toh (Singaporean strain)

text: adult testis ORESTES (TLL)

text: pGEM-T


text: University of Puerto Rico

text: live

text: Generic

text: Directional cDNA library. Sequencing carried out by W.O. McMillan, University of Puerto Rico

text: Various

text: wing disc various stages

text: Heliconius erato petiverana

text: Heliconius erato wing disc


text: Cornell University

text: live

text: Generic

text: Reproductive tract

text: Drosophila simulans

text: Female

text: FemaleRT

text: Plasmid


text: University of Illinois at Urbana-Champaign

text: live

text: Generic

text: Williams 82

characteristic: cultivar

text: The cDNA library was constructed, normalized and subtracted by the W.M. Keck Center, University of Illinois Urbana-Champaign as described in Bonaldo, M.F., Lennon, G. and Soares, M.B. (1996), Genome Research 6(9): 791-806. Messenger RNA extracted from Fusarium solani and soybean roots infected with Fusarium solani was converted to ds-cDNA with tagged oligo-dT used to identify the source of the ESTs where possible: ACGCA18(T) = Fusarium solani; TCCGA18(T) = soybean roots infected with Fusarium solani. The ds-cDNAs were size selected (more than 450 bp), adaptored with EcoRI adaptors and digested with NotI. The ds-cDNA were then directionally cloned into EcoRI-NotI digested pBluescriptII SK(+) phagemid vector. The normalized library was subtracted with cDNA synthesized from non-inoculated healthy soybean root mRNA. Clones were sequenced from the 3' end using standard primer M13 reverse n48.

characteristic: lab-host

text: Root and fungal culture

text: Glycine max/Fusarium solani f. sp. glycines mixed EST library

text: EcoRI

text: NotI

text: Plasmid

text: pBSIISK(+)


characteristic: External Id

text: Universite Laval

text: live

text: Generic

text: cDNA was prepared from 5 mg of poly A+ selected RNA and was directionally ligated into the pBluescript II SK (+) XR vector (Stratagene), transformed by electroporation into DH10B cells (In vitrogen) for propagation

text: Actively elongating roots

text: E. coli DH10B cells

text: Roots from 60 cm tall seedlings.

text: Picea glauca

text: Eco-RI

text: Xho-I

text: Hermaphrodite

text: Tender roots tips free of michorizee

text: GQ013: Elongating ROOTS tips - saplings

characteristic: title

text: Plasmid

text: pBluescript II SK (+) XR


text: Universite Laval

characteristic: INSDC center name

text: live

text: Generic

text: Primary & secondary shoot, secondary phloem pooled from plants fertilized with low and high NH4NO3

text: E. coli DH10B cells

text: Main stem region producing secondary growth on 60 cm tall seedlings

text: Picea glauca

text: Eco-RI

text: Xho-I

text: Hermaphrodite

text: Xylem, pith, cambium, phloem, bark

text: GQ016: Primary, secondary SHOOT -N fertil. Treatments

text: Plasmid

text: pBluescript II SK (+) XR

characteristic: vector


text: Universite Laval

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Collected after two-weeks on maturation medium containing ABA

text: E. coli DH10B cells

text: Whole intact somatic embryos comprised of senescing suspensor and bullet stage embryonic head

text: Picea glauca

text: Eco-RI

text: Xho-I

text: Hermaphrodite

text: Whole embryo

text: GQ017: Immature somatic EMBRYOS

text: Plasmid

text: pBluescript II SK (+) XR


text: Universite Laval

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Roots from plants fertilized with low and high NH4NO4

text: E. coli DH10B cells

text: whole root systems form 15 cm tall seedlings

text: Picea glauca

text: Eco-RI

text: Xho-I

text: Hermaphrodite

text: Whole roots systems, predominantly free of michorizee

text: GQ018: Clean ROOTS systems - N treatments

text: Plasmid

text: pBluescript II SK (+) XR


text: Universite Laval

text: live

text: Generic

text: Tissues were pooled from several plants receiving daily fertilization varying potasium levels (either 0.02 mM or 2 mM PO4), collected at 4 hours, 24 hours, 2 days, 4 days or 7 days of treatment. cDNA was prepared from 5 mg of poly A+ selected RNA and was directionally ligated into the pBluescript II SK (+) XR vector (Stratagene), transformed by electroporation into DH10B cells (In vitrogen) for propagation

text: E. coli DH10B cells

text: Whole root systems form 15 cm tall, 4 month old seedlings produced by somatic embryogenesis

text: Picea glauca

text: Eco-RI

text: Xho-I

text: Hermaphrodite

characteristic: strain

characteristic: tissue-type

text: GQ019: Clean ROOTS systems - P treatments

text: Plasmid

text: pBluescript II SK (+) XR


text: Universite Laval

text: live

text: Generic

characteristic: NCBI submission package

text: Tissues were pooled from several plants grown under 20h light/4h dark and collected every 2 hours over a period of 28 hours (4 dark time points and 11 time points in light). cDNA was prepared from 5 mg of poly A+ selected RNA and was directionally ligated into the pBluescript II SK (+) XR vector (Stratagene), transformed by electroporation into DH10B cells (In vitrogen) for propagation

characteristic: description

text: E. coli DH10B cells

text: whole root systems form 15 cm tall, 4 month old seedlings produced by somatic embryogenesis

text: Picea glauca

text: Eco-RI

text: Xho-I

text: Hermaphrodite

text: GQ020: Clean ROOTS systems -Diurnal cycle

text: Plasmid

text: pBluescript II SK (+) XR


text: Universite Laval

text: live

text: Generic

text: Non-lignified xylem tissues from larger roots in early part of growing season (June).

characteristic: dev-stage

text: E. coli DH10B cells

text: Roots from 9 year old trees measuring approximately 4.5 m tall, and 10 cm in diameter.

characteristic: organ

text: Picea glauca

text: Eco-RI

text: Xho-I

text: Hermaphrodite

text: Three trees Unknown

text: Differentiating xylem from roots 1 cm in diameter or larger

text: GQ022: ROOT XYLEM - mature trees

text: Plasmid

text: pBluescript II SK (+) XR


text: Universite Laval

text: live

text: Generic

text: cDNA was prepared from 5 mg of poly A+ selected RNA and was directionally ligated into the pBluescript II SK (+) XR vector (Stratagene), transformed by electroporation into DH10B cells (In vitrogen) for propagation

text: Shoot comprised of primary and secondary growth (no needles) of annual flush pooled from several trees collected over a natural 15h40min light / 9h20min dar

text: E. coli DH10B cells

text: Stem annual flush from top of 10 year old trees, approximately 5 m tall and 12 cm in diameter

text: Picea glauca

characteristic: organism

text: Eco-RI

text: Xho-I

text: Hermaphrodite

characteristic: sex

text: Several trees Unknown

text: Pith, xylem, cambium, phloem and bark. Needles were removed.

text: GQ025: Annual flush SHOOTS diurnal cycle- trees

text: Plasmid

text: pBluescript II SK (+) XR


text: Universite Laval

text: live

text: Generic

text: Young and mature needles were pooled

text: E. coli DH10B cells

characteristic: lab-host

text: Foliage stem from 60 cm tall seedlings

text: Picea glauca

text: Eco-RI

text: Xho-I

text: Hermaphrodite

text: Needles from plant fertilized with 0 mM NH4NO3 (low N) AND with 10 mM NH4NO3 (high N)

text: GQ026: NEEDLES - N fertilization treatments

text: Plasmid

text: pBluescript II SK (+) XR


characteristic: External Id

text: Universite Laval

text: live

text: Generic

text: Mature embryo obtained by growing immature somatic embryonic massess on media containing ABA for 8 weeks

text: E. coli DH10B cells

text: Whole intact somatic embryos comprised of cotyledonary, root, and apical primordia and hypocotyl

text: Picea glauca

text: Eco-RI

text: Xho-I

text: Hermaphrodite

text: Whole embryo

text: GQ027: Mature Somatic EMBRYOS

text: Plasmid

text: pBluescript II SK (+) XR


text: Uppsala University/Swedish University of Agricultural Sciences

text: live

text: Generic

text: The cDNA libraries were created with the Superscript Plasmid System (Invitrogen).

text: ElectroMAX DH10B (Invitrogen)

text: brain/testis

text: Gallus gallus

text: Hind III

text: EcoRI

text: female/male

text: Red junglefowl/White Leghorn

text: WL/RJ Phraped ESTs

characteristic: title

text: plasmid


text: Madras Veterinary College

characteristic: INSDC center name

text: live

text: Generic

text: Mononuclear cells

characteristic: cell type

text: Buffalo spleen cells stimulated with concanavalin A and prepared 24 hours post stimulation.

text: Spleen cells

text: Bubalus bubalis

text: Sfi I

text: Sfi I

text: male

text: Non descript

text: cDNA library from mitogen stimulated buffalo spleen cells

text: Plasmid

text: pDNR LIB


text: Swammerdam Institute for Life Sciences, University of Amsterdam

characteristic: INSDC first public

text: live

text: Generic

text: Mitchell

characteristic: cultivar

text: Petal limbs

text: Petunia x hybrida

text: Petunia Uni-ZAP?XR custom cDNA library


text: The Natural History Museum

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: mRNA, extracted from schistosome-unexposed 3017 (resistant) snails was used as a template for RT-PCR with random primers to generate cDNA fragments. These were cloned and sequenced using M13F. Primer sequences were removed from sequence.

text: Haemopoietic organ

text: Biomphalaria glabrata

text: Hermaphrodite

text: NHM 3017

text: BgORESTES unexposed NHM 3017 Haemopoietic organ 2

text: pGEM

characteristic: vector


text: The Natural History Museum

text: live

text: Generic

characteristic: NCBI submission model

text: mRNA, extracted from schistosome-exposed 3017 (resistant) snails was used as a template for RT-PCR with random primers to generate cDNA fragments. These were cloned and sequenced using M13F. Primer sequences were removed from sequence.

text: Haemopoietic organ

text: Biomphalaria glabrata

text: Hermaphrodite

text: NHM 3017

characteristic: strain

text: BgORESTES schistosome-exposed NHM 3017 Haemopoietic organ 2

text: pGEM


text: CIRAD

text: live

text: Generic

characteristic: NCBI submission package

text: Penkelon Noir

text: Musa X paradisiaca Triploid AAB ; ESTs obtained from reciprocal SSH libraries of BSV activated and non-activated vitroplantlet.

characteristic: description

text: Vitro Plantlet

text: Musa AAB Group

text: EcoR I

text: EcoR I

text: Hermaphrodite

text: whole plantlet

characteristic: tissue-type

text: BSV activation

text: Plasmid

text: pGEM-T Easy


text: (Wang, Yan) College of Life Sciences, Shaanxi Normal University; (Tian) Hangzhou genomics Research and Development Institute

text: live

text: Generic

text: mRNA isolation used PolyATtract(r) mRNA Isolation Systems (PROMEGA, CAT No.Z5200) and pBluescript(r) XR cDNA Library Construction Kit (Invitrogen, CAT No200455.) to construct the cDNA library.

text: cultured about 1mon-3mon

characteristic: dev-stage

text: Whole Youth Plant

characteristic: organ

text: Salvia miltiorrhiza

characteristic: organism

text: EcoRI

text: XholI

text: hermaphrodite

text: Whole Youth Plant

text: cDNA Library of Salvia miltiorrhiza

text: Plasmid

text: pBluescript


characteristic: External Id

text: Huazhong Agricultural University of China

text: live

text: Generic

text: mRNA was isolated from backfat of Duroc and Tongcheng pig, and mRNA differential display was conducted to compare the gene differential expression, then differentially expressed bands were reamplfied, identified by Reverse Northern Dot Blot, then cloned to T-vector and sequenced, at last, using semi-quantitative RT-PCR further identified.

text: multiple stages

text: E.coli JM109

characteristic: lab-host

text: multiple organs

text: Sus scrofa

text: male and female

characteristic: sex

text: Duroc and Tongcheng pigs

text: backfat tissue

text: Normalized cDNA from backfat tissue of pigs

text: Plasmid

text: pMD-18 T vector


text: Teagasc, Agriculture and Food Development Authority

characteristic: INSDC center name

text: live

text: Generic

text: A bovine ovarian cortex cDNA library was constructed using the Lambda Zap Express/Gigapack cloning kit (Stratagene cloning systems). cDNA synthesis was carried out using an oligo-(dT) primer for the reverse transcription of 5(g of mRNA and the library was constructed by directional cloning EcoR1-Xho1 based on manufacturers instructions. An insert:vector ligation ratio of 1:5 was chosen as most optimum. The lambda library was packaged with Gigapack III gold packaging extracts and plated on the E. coli cell line XL1-Blue MRF'.

text: Day 2, 6, 8 and 14 of the oestrus cycle

text: Ovarian cortex

text: Bos taurus

text: Female

text: Ovarian cortex

text: Bovine Lambda Zap Express ovarian cortex cDNA library

characteristic: title

text: Phage

text: Lambda ZAP Express


text: The Natural History Museum

characteristic: INSDC first public

text: live

text: Generic

text: mRNA, extracted from infected 3032 (susceptible) snails was used as a template for RT-PCR with random primers to generate cDNA fragments. These were cloned and sequenced using M13F. Primer sequences were removed from sequence.

text: haemopoietic organ

text: Biomphalaria glabrata

text: Hermaphrodite

text: NHM 3032

text: BgORESTES infected NHM 3032 haemopoietic organ

text: pGEM


text: The Natural History Museum

characteristic: INSDC last update

text: live

text: Generic

text: mRNA, extracted from uninfected 3032 (susceptible) snails was used as a template for RT-PCR with random primers to generate cDNA fragments. These were cloned and sequenced using M13F. Primer sequences were removed from sequence.

text: haemopoietic organ

text: Biomphalaria glabrata

text: Hermaphrodite

text: NHM 3032

text: BgORESTES uninfected NHM 3032 haemopoietic organ

text: pGEM


text: Chinese Agricultural University

text: live

characteristic: INSDC status

text: Generic

text: skeletal muscle

characteristic: cell type

text: A porcine skeletal muscle forward subtracted cDNA library was constructed by suppression subtractive hybridisation (SSH). We firstly set up two RNA pools with muscular RNA come from double-muscled pig(DM) and non-double-muscled pig(NDM) individually, and then constructed the subtracted cDNA library by suppression subtractive hybridization (SSH).

text: 2.5 months piglet

text: DH10B (Life Technologies)

text: musculus longissimus dorsi

text: Sus scrofa

text: Rsa I

text: hermaphrodite

text: purebred

text: musculus longissimus dorsi

text: Forward subtracted cDNA library from skeletal muscle of double-muscled large white pig

text: Plasmid


text: Concordia University, Department of Biology

text: live

text: Generic

characteristic: NCBI submission model

text: Aerial parts (crown and leaf) of wheat cultivar Norstar from control and long exposure times to low temperature. 4 mRNA populations were combined before constructing the library; 7 days non-acclimated plants and 1, 23, and 53 days cold-acclimated at 4C. Non-acclimated and cold-acclimated plants were grown in vermiculite This is the only library that was done according to the Invitrogen manual, and therefore, a percentage of clones will not have the 3 prime end because of NotI digestion within the cDNA.

text: Crown and leaf

text: Triticum aestivum

text: Triticum aestivum FGAS: Library 2 Gate 3

characteristic: vector


text: Children's Memorial Research Center

text: live

text: Generic

characteristic: NCBI submission package

text: UI-R-DY1 is a normalized cDNA library containing the following tissue(s): Rat Cartilage from Femur and Tibia. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CTAATGGACG. The Rat cartilage tissue was provided by Dr Jeff Stevens at the University of Iowa.

text: ADULT

text: DH10B (Life Technologies)

text: Femur and Tibia

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

characteristic: strain

text: Cartilage

text: Phagemid


text: Sunmoon University

text: live

text: Generic

text: Cheong-Ryong Cho

characteristic: cultivar

text: cDNA library made with cold treated mRNA

characteristic: description

text: whole plant

text: Capsicum annuum

text: Capsicum annuum Uni-ZAP XR cDNA Library


text: Baylor College of Medicine

text: live

text: Generic

text: Mature, healthy leaflets were collected from a single tree at the USDA/ARS National Clonal Germplasm Repository for Pecans and Hickories in Somerville, Texas. Leaflets were immediately frozen with dry ice and brought back to the lab where they were subsequently stored at -80 C. mRNA was extracted from frozen leaflet samples and cDNA was prepared from polyA+ enriched RNA. The library was constructed using the Invitrogen ZAP-cDNA Gigapack Gold Cloning Kit.

text: Mature leaflet

characteristic: organ

text: Carya illinoinensis

text: Pecan Leaflet-Field


text: USDA-Agricultural Research Service

text: live

text: Generic

text: Primary library

text: 5-, 7-, and 9-day fry

characteristic: dev-stage

text: whole animal

text: Ictalurus punctatus

characteristic: organism

text: Sal 1

text: Not 1

text: plasmid


characteristic: External Id

text: USDA-Agricultural Research Service

text: live

text: Generic

text: Normalized library

text: 5-, 7-, and 9-day fry

characteristic: lab-host

text: whole animal

text: Ictalurus punctatus

text: Sal 1

text: Not 1

text: plasmid


text: Huazhong agricultural university of China

characteristic: INSDC center name

text: live

text: Generic

text: Seedings of the inbred lines with two leaves were submerged in hypoxia buffer. SSH libraries were constructed with cDNA samples from roots. Both forward and reverse subtractions were performed for each inbred line, and 105 positive clones induced by hypoxia were selected by differential screening.

text: seedling

text: multiple organs

text: Zea mays

text: hermaphrodite

characteristic: sex

text: Maize inbred Hz32

text: root

characteristic: tissue-type

text: Suppression Subtractive Hybridization Library of Maize Inbred Hz32

text: Plasmid

text: T-easy


characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: seedling

text: multiple organs

text: Zea mays

text: hermaphrodite

text: Maize inbred Mo17

text: root

text: Suppression Subtractive Hybridization Library of Maize Inbred MO17

characteristic: title

text: Plasmid

text: T-easy


text: Department of Biotechnology

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Early passage neurosphere

characteristic: cell type

text: A cDNA library was constructed in pCMVsport6.0 from RNA isolated from neurospheres of adult male and female mice. Custom normalized cDNA library by Invitrogen/ResGen

text: Adult

text: Adult brain

text: Mus musculus

text: MALE

text: Lateral wall of lateral ventricle

text: Mouse Neurosphere Normalized cDNA library


text: Department of Biotechnology

text: live

text: Generic

characteristic: NCBI submission package

text: Hematopoietic progenitor/stem cells immortalized by Lhx2

text: hematopoietic stem cell-like cell line BM-HPC5

characteristic: cell-line

text: Preamplified custom cDNA library by Invitrogen/ResGen

text: adult

text: Bone Marrow

text: Mus musculus

text: Male and Female

characteristic: strain

text: Bone Marrow

text: Mus Musculus hematopoietic BM-HPC5 cDNA library

characteristic: vector


text: University of Maryland

text: live

text: Generic

text: INRA clone 717 1-B4 and ethylene insensitive line R1-20

characteristic: cultivar

text: Developing vegetative apical buds were collected after various time intervals of SD treatment. Total RNA was isolated and used for cDNA-AFLP profiling with ApoI and Mse1. RNA was pooled for buds representing initiation (1, 2,3 weeks of SD treatment), development (4,5,6 weeks of SD treatment), maturation and dormancy (7 and 8 weeks of SD treatment) and dormancy (9 and 12 weeks SD with low temperature). Transcript derived fragments (TFDs) that showed differential abundance during bud development and dormancy or between genotypes was excised from the cDNA-AFLP gel, amplified by PCR and TA cloned into either pGEM-T or pGEM-T Easy. Cloned TFDs were then sequenced using sequencing primers. The project was supported by the National Research Initiative of the USDA Cooperative State Research, Education and Extension Service, grant number # (2003-35304-13161).

characteristic: description

text: Initiated, maturing and dormant

characteristic: dev-stage

text: Developing vegetative bud

characteristic: organ

text: Populus tremula x Populus alba

text: Populus tremula X Populus alba developing vegetative bud cDNA Library

text: Plasmid

text: pGEM-T or pGEM-T Easy


characteristic: External Id

text: Tufts University School of Veterinary Medicine

characteristic: INSDC center name

text: live

text: Generic

text: most cell types

text: The whole bodies (cephalotorax and tail) of six juvenile shrimp injected with the China strain of white spot syndrome virus and six control (uninjected) siblings were used for RNA isolation. The CLONTECH PCR-SelectTM cDNA subtraction kit was used for library construction. PCR products were ligated to PCR-TRAP(r) vector and transformed in GH-competent cells. Lgh and Rgh primers were used for PCR reactions.

text: juveniles (~3.1 grams)

text: whole juvenile shrimp

text: Penaeus vannamei

characteristic: organism

text: not determined

text: most tissue types

text: Subtracted cDNA library of WSSV-injected shrimp

text: PCR-TRAP(r)


text: Madras Veterinary College

characteristic: INSDC first public

text: live

text: Generic

text: Mononuclear cells

text: Buffalo spleen cells stimulated with concanavalin A and prepared 24 hours post stimulation.

text: Spleen cells

text: Bubalus bubalis

text: Sfi I

text: Sfi I

text: male

characteristic: sex

text: Non descript

text: cDNA library from concanavalin A stimulated buffalo spleen cells

text: Plasmid

text: pDNR LIB


text: North Carolina State University

characteristic: INSDC last update

text: live

text: Generic

text: vitellogenic

text: fat body plus ovary

text: Dermacentor variabilis

text: female

text: American Dog Tick fat body plus ovary library

characteristic: title

text: plasmid


text: National Fisheries Research & Development Institute

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Adult

text: E.coli SOLR

characteristic: lab-host

text: Digestive diverticula

text: Haliotis discus

text: EcoR I

text: Xho I

text: Haliotis discus cDNA library (DGT)

text: Phagemid

text: pBluescript SK(-)


text: live

text: Generic

characteristic: NCBI submission package

text: Adult

text: E.coli SOLR

text: Rectangular muscle

text: Haliotis discus

text: EcoR I

text: Xho I

text: Haliotis discus cDNA library (RM)

text: Phagemid

text: pBluescript SK(-)


text: live

text: Generic

text: Adult

text: E.coli SOLR

text: Gonad of male

text: Haliotis discus

text: EcoR I

text: Xho I

text: Haliotis discus cDNA library (GOM)

text: Phagemid

text: pBluescript SK(-)


text: live

text: Generic

text: Adult

text: E.coli SOLR

text: Gonad of female

text: Haliotis discus

text: EcoR I

text: Xho I

text: Haliotis discus cDNA library (GOF)

text: Phagemid

text: pBluescript SK(-)


text: live

text: Generic

text: Adult

text: E.coli SOLR

text: Hepatopancreas

text: Haliotis discus

text: EcoR I

text: Xho I

text: Haliotis discus cDNA library (VHP)

text: Phagemid

text: pBluescript SK(-)


text: Oklahoma State University

text: live

text: Generic

text: Lonestar tick female salivary gland cDNA library 1, 50-200 mg feeding adults. Constructed by Richard Essenberg, Department of Biochemistry and Molecular Biology at Oklahoma State University, Stillwater OK.

text: salivary gland

text: Amblyomma americanum

text: EcoRI

text: SfiIb

text: female

text: cloneTech pTriplEx2


text: Oklahoma State University

text: live

text: Generic

text: Lonestar tick female salivary gland cDNA library 2, unfed adults, feeding adults, replete adults. Constructed by Richard Essenberg, Department of Biochemistry and Molecular Biology at Oklahoma State University, Stillwater OK.

text: salivary gland

text: Amblyomma americanum

text: EcoRI

text: SfiIb

text: female

text: cloneTech pTriplEx2


text: Oklahoma State University

text: live

text: Generic

text: cDNA library from 3-day feeding ticks constructed by Stratagene Lambda ZAP from RNA isolated by Glenn Needham, Ohio State University, converted to plasmid form

text: adult, 3-day feeding

text: sheep

text: salivary gland

text: Amblyomma americanum

text: XhoI

text: EcoRI

text: female

text: plasmid

text: pBluescript SK-


text: Oklahoma State University

text: live

text: Generic

text: Lonestar tick female salivary gland cDNA library 4, 50-200 mg feeding adults, normalized from Lib1. Constructed by Richard Essenberg, Department of Biochemistry and Molecular Biology at Oklahoma State University, Stillwater OK.

text: salivary gland

text: Amblyomma americanum

text: EcoRI

text: SfiIb

text: female

text: cloneTech pTriplEx2


text: Michigan State University

text: live

text: Generic

text: US H20

text: cDNAs were derived from reverse transcription of mRNA isolated from seeds germinating in solution.

text: seedling

text: seedlings 4-day

text: Beta vulgaris

text: Sugar Beet

characteristic: strain

text: whole tissue

characteristic: tissue-type

text: Sugar Beet differential display cDNA


text: Michigan State University

text: live

text: Generic

text: USH20 and EL51

characteristic: cultivar

text: cDNAs were derived from reverse transcription of mRNA isolated from seedlings inoculated with Rhizoctonia solani AG2-2

text: 4 - 6 leaf stage

text: E. coli

text: young plants

text: Beta vulgaris

text: Sugar Beet

text: Hypocotyl and root

text: Sugar Beet / Rhizoctonia cDNA-AFLP clones

characteristic: vector


text: National Institute of Allergy and Infectious Diseases

text: live

text: Generic

text: Secretory

characteristic: cell type

text: PCR-based cDNA library made with SMART cDNA library construction kit from Clontech

characteristic: description

text: Adult

characteristic: dev-stage

text: Salivary gland

text: Anopheles gambiae

text: Male

text: Salivary gland

text: Adult male salivary glands 0-2 days after emergence

text: Phage

text: Lambda TriplEx2


characteristic: External Id

characteristic: INSDC center name

text: live

text: Generic

text: Secretory

text: Adult

text: Salivary gland

characteristic: organ

text: Anopheles gambiae

text: Female

text: Salivary gland

text: Adult female salivary glands 0-2 days after emergence

text: Phage

text: Lambda TriplEx2


text: University of New Mexico

characteristic: INSDC first public

text: live

text: Generic

text: mRNA was harvested from cercariae, adults and 48-hour cultivated sporocysts of S. mansoni. cDNA was generated using oligo-dT primers. The sporocyst cDNA was subtracted against the combined non-sporocyst cDNA using the PCR-Select Suppression Subtractive Hybridization kit (SSH) (Clontech, Palo Alto, CA). The resulting library was cloned into pCR2.1 (Invitrogen, Carlsbad, CA) and sequenced on both strands using plasmid-specific primers (M13 rev and M13 -20).

text: Biomphalaria glabrata

text: whole body, sporocyst

text: Schistosoma mansoni

characteristic: organism

text: EcoRI

text: EcoRI

text: S. mansoni sporocyst SSH library

text: plasmid


text: Waksman Institute, Rutgers University

characteristic: INSDC last update

text: live

text: Generic

text: Poly(A)-selected mRNAs from Caenorhabditis remanei were converted to cDNAs with reverse transcriptase and oligo(dT) primers. EcoRI linker-adapters were ligated to the cDNAs, which were then ligated into the unique EcoRI site of the plasmid vector pTriplEx2. Recombinant plasmids were transformed into E. coli strain DH5alpha.

text: Mixed stages

text: E. coli DH5alpha

text: Whole animal

text: Caenorhabditis remanei

text: EcoRI

text: Pooled male and female

characteristic: sex

text: WSSP C. remanei cDNA Library

characteristic: title

text: Plasmid


text: Insitute of Vegetable Science, Zhejiang University of China

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: pollen development

text: flower buds

text: Brassica rapa

text: buds

text: cDNA of flower buds cultivar ZUBajh97-01B


text: live

text: Generic

characteristic: NCBI submission package

text: pollen development

text: flower buds

text: Brassica rapa

text: buds

text: cDNA of flower buds cultivar ZUBajh97-01A


text: IRNASA-CSIC

text: live

text: Generic

text: Wassilewskija

text: cDNA fragments of genes expressed during seed germination from cDNA-AFLP experiment were cloned in pGEM-T vector

text: Germination

text: Seed imbibed for 24 hours

text: Arabidopsis thaliana

text: cDNA-AFLP germinating seeds

text: Plasmid

text: pGemT