text: Universidade de Sao Paulo

characteristic: INSDC center name

text: live

text: Generic

text: minilibrary constructed using low-stringency RT-PCR and consensus-degenerate primer

text: Adult

characteristic: dev-stage

text: Schistosoma mansoni

text: BH

text: f24i S. mansoni adult mini-library, Fietto/DeMarco/Verjovski-Almeida

text: Bluescript SK


status: PUBLIC

characteristic: External Id

text: Universidade de Sao Paulo

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

characteristic: description

text: Adult

tag: attribute

text: Schistosoma mansoni

characteristic: organism

text: BH

characteristic: strain

text: f19 S. mansoni adult mini-library, Fietto/DeMarco/Verjovski-Almeida

characteristic: title

text: Bluescript SK

characteristic: vector


text: Universidade de Sao Paulo

text: live

text: Generic

text: Adult

text: Schistosoma mansoni

text: BH

text: f3 S. mansoni adult mini-library, Fietto/DeMarco/Verjovski-Almeida

text: Bluescript SK


text: Universidade de Sao Paulo

text: live

text: Generic

text: Adult

text: Schistosoma mansoni

text: BH

text: f24s S. mansoni adult mini-library, Fietto/DeMarco/Verjovski-Almeida

text: Bluescript SK


text: Universidade de Sao Paulo

text: live

text: Generic

text: Adult

text: Schistosoma mansoni

text: BH

text: f33 S. mansoni adult mini-library, Fietto/DeMarco/Verjovski-Almeida

text: Bluescript SK


text: Universidade de Sao Paulo

text: live

text: Generic

text: Adult

text: Schistosoma mansoni

text: BH

text: f23i S. mansoni adult mini-library, Fietto/DeMarco/Verjovski-Almeida

text: Bluescript SK


text: Universidade de Sao Paulo

text: live

text: Generic

text: Adult

text: Schistosoma mansoni

text: BH

text: f23s S. mansoni adult mini-library, Fietto/DeMarco/Verjovski-Almeida

text: Bluescript SK


text: Universidade de Sao Paulo

text: live

text: Generic

text: Adult

text: Schistosoma mansoni

text: BH

text: h72 S. mansoni adult mini-library, Fietto/DeMarco/Verjovski-Almeida

text: Bluescript SK


text: Universidade de Sao Paulo

text: live

text: Generic

text: Adult

text: Schistosoma mansoni

text: BH

text: h81 S. mansoni adult mini-library, Fietto/DeMarco/Verjovski-Almeida

text: Bluescript SK


text: Universidade de Sao Paulo

text: live

text: Generic

text: Adult

text: Schistosoma mansoni

text: BH

text: h91 S. mansoni adult mini-library, Fietto/DeMarco/Verjovski-Almeida

text: Bluescript SK


text: Universidade de Sao Paulo

text: live

text: Generic

text: Adult

text: Schistosoma mansoni

text: BH

text: h102 S. mansoni adult mini-library, Fietto/DeMarco/Verjovski-Almeida

text: Bluescript SK


text: Universidade de Sao Paulo

text: live

text: Generic

text: Adult

text: Schistosoma mansoni

text: BH

text: h112 S. mansoni adult mini-library, Fietto/DeMarco/Verjovski-Almeida

text: Bluescript SK


text: Universidade de Sao Paulo

text: live

text: Generic

text: Adult

text: Schistosoma mansoni

text: BH

text: f28i S. mansoni adult mini-library, Fietto/DeMarco/Verjovski-Almeida

text: Bluescript SK


text: Universidade de Sao Paulo

text: live

text: Generic

text: Adult

text: Schistosoma mansoni

text: BH

text: h122 S. mansoni adult mini-library, Fietto/DeMarco/Verjovski-Almeida

text: Bluescript SK


text: Universidade de Sao Paulo

text: live

text: Generic

text: Adult

text: Schistosoma mansoni

text: BH

text: r39 S. mansoni adult mini-library, Fietto/DeMarco/Verjovski-Almeida

text: Bluescript SK


text: Universidade de Sao Paulo

text: live

text: Generic

text: Adult

text: Schistosoma mansoni

text: BH

text: h142 S. mansoni adult mini-library, Fietto/DeMarco/Verjovski-Almeida

text: Bluescript SK


text: Universidade de Sao Paulo

text: live

text: Generic

text: minilibrary constructed using low-stringency RT-PCR and consensus-degenerate primer

text: Adult

text: Schistosoma mansoni

text: BH

text: h185 S. mansoni adult mini-library, Fietto/DeMarco/Verjovski-Almeida

text: Bluescript SK


text: Universidade de Sao Paulo

text: live

text: Generic

text: Adult

text: Schistosoma mansoni

text: BH

text: h191 S. mansoni adult mini-library, Fietto/DeMarco/Verjovski-Almeida

text: Bluescript SK


text: Universidade de Sao Paulo

text: live

text: Generic

text: Adult

text: Schistosoma mansoni

text: BH

text: h191s S. mansoni adult mini-library, Fietto/DeMarco/Verjovski-Almeida

text: Bluescript SK


text: Universidade de Sao Paulo

text: live

text: Generic

text: Adult

text: Schistosoma mansoni

text: BH

text: f4i S. mansoni adult mini-library, Fietto/DeMarco/Verjovski-Almeida

text: Bluescript SK


text: Michigan State University

text: live

text: Generic

characteristic: cultivar

text: The mRNA samples used for this library were derived from seedlings germinated for 4 days in 150mM NaCl and 200mM mannitol. The mRNA pool was used as template for double stranded cDNA synthesis using the Stratagene pBluescript XR cDNA synthesis and library kit. The resulting cDNA was used as a tester for subtraction against a driver cDNA population derived from 4-day old seedlings germinated in moist filter paper. Subtraction was performed using the Invitrogen Photobiotin-Streptavidin subtractor kit. The cDNA library was generated by directional ligation of the subtracted cDNAs in the EcoRI and XhoI sites of pBluescript XR vector (Stratagene).

text: 4-day germination under stress (salt/NaCl, osmotic/Mannitol)

text: XL1-Blue MRF'

characteristic: lab-host

text: seeds

characteristic: organ

text: Beta vulgaris

text: EcoRI

text: XhoI

text: Sugar Beet

text: whole seedlings

characteristic: tissue-type

text: Sugar Beet stress germination cDNA library (subtracted)

text: plasmid

text: pBluescript II KS (+)


text: NRC Institute for Marine Biosciences

characteristic: INSDC center name

text: live

text: Generic

text: adult

text: kidney

text: Salmo salar

text: Atlantic Salmon kidney

text: Lambda ZAP II


text: NRC Institute for Marine Biosciences

text: live

text: Generic

text: adult

characteristic: dev-stage

text: liver

text: Salmo salar

text: Atlantic Salmon liver

text: Lambda ZAP II


characteristic: External Id

text: NRC Institute for Marine Biosciences

text: live

text: Generic

text: adult

text: spleen

text: Salmo salar

text: Atlantic Salmon spleen

text: Lambda ZAP II


text: Zhongshan Ophthalmic Center

characteristic: INSDC first public

text: live

text: Generic

text: Mus musculus

text: rds Mouse Retina Differential Expression Library


text: Eastern Hospital and Institute of Hepatobiliary Surgery

characteristic: INSDC last update

text: live

text: Generic

text: Homo sapiens

text: hepatocellular carcinoma

text: Homo sapiens hepatocellular carcinoma


text: Hubei College of Traditional Chinese Medicine

text: live

characteristic: INSDC status

text: Generic

text: It was prepared with arbitrarily Primed PCR fingerprinting of RNA.

text: Aging

text: Rattus norvegicus

text: Sprague-Dawley

text: brain(hippocampus)

text: Rat brain cDNA library generated with RNA fingerprinting

text: Plasmid

text: T/A Easy Vector


text: University of Notre Dame

text: live

text: Generic

characteristic: NCBI submission model

text: cDNA fragment was obtained by differential display while comparing Plasmodium gallinaceum-infected midguts

text: adult female

text: Aedes aegypti

text: female

characteristic: sex

text: RED

text: midgut

text: RED midgut


text: University of Notre Dame

text: live

text: Generic

characteristic: NCBI submission package

characteristic: description

text: adult female

text: Aedes aegypti

text: female

text: MOYO-R

text: midgut

text: MOYO-R midgut


text: University of Notre Dame

text: live

text: Generic

text: adult female

text: Aedes aegypti

characteristic: organism

text: female

text: midgut

text: bulk 2 F2 midgut


text: Dept of AFNS, University of Alberta

text: live

text: Generic

text: Epithelial

characteristic: cell type

text: Young adult

text: Abomasum

text: Bos taurus

text: EcoR I

text: Xho I

text: Two males and one female mixed

text: Gastrointestinal tissue (GIT)

text: Bovine Abomasum cDNA Library

characteristic: title

text: Phage


text: Washington University School of Medicine

text: live

text: Generic

text: This library is assigned to clones that are of uncertain origin due to an error during re-array, prepping, and/or analysis. Samples assigned this library are still genuine Glycine Max cDNA sequence, but we are unsure of its source and identification. Due to the clone id being incorrectly assigned, THESE CLONES ARE NOT AVAILABLE TO ORDER.

text: Glycine max

text: Unknown Library Type


text: National Institute of Diabetes and Digestive and Kidney Diseases

text: live

text: Generic

text: Erythroid Cells

text: Primary Culture of Peripheral Blood Mononuclear Cells

characteristic: cell-line

text: 65,000 proliferating erythroid cells from the buffy coat of a blood donation were obtained by flow cytometric separation after a 5-day culture period in the presence of erythropoietin. Total RNA was purified from the sorted cell population using TRIzol reagent. RNA (0.3 ug) was converted into double stranded cDNA using Clontech's CapFinder cDNA Library Construction Kit (Clontech) according to the manufacturer's protocol and cloned into EcoRI digested Lambda Zap II vector (Stratagene). The phage library was amplified once prior to in vivo excision in SOLR cells. Individual colonies were grown, and the cDNA inserts were sequenced in high throughput (NIH intramural sequencing center http://www.nisc.nih.gov/).

text: Progenitor; EPO responsive CD71++++

text: SOLR

text: blood

text: Homo sapiens

text: EcoRI

text: EcoRI

text: blood

text: Hembase; Erythroid Progenitor Cells (LCB:ax library)

text: plasmid

text: Lambda ZAP II

characteristic: vector


text: University of North Carolina

text: live

text: Generic

text: tendon internal fibroblasts

text: treated with estrogen as B-estradiol, grown to density-arrested quiescence then treated with estrogen and mechanically loaded in a Flexercell Strain Unit at 1 Hz, 0.05 average strain for 6 hr

text: Gallus gallus

text: Gallus gallus tendon internal fibroblasts


text: Ludwig Institute for Cancer Research

text: live

text: Generic

text: A mini-library was made by cloning products derived from ORESTES PCR (U.S. Letters Patent application No. 196,716 - Ludwig Institute for Cancer Research) profiles into the pUC 18 vector. Reverse transcription of tissue mRNA and cDNA amplification were performed under low stringency conditions.

text: Adult

text: breast

characteristic: organ

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: Ludwig Institute for Cancer Research

text: live

text: Generic

text: Adult

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: Ludwig Institute for Cancer Research

text: live

text: Generic

text: Adult

text: colon

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: Ludwig Institute for Cancer Research

characteristic: INSDC secondary accession

text: live

text: Generic

characteristic: SRA accession

text: Adult

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: Ludwig Institute for Cancer Research

characteristic: INSDC center name

text: live

text: Generic

text: Adult

characteristic: dev-stage

text: leiomios

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


characteristic: External Id

text: Ludwig Institute for Cancer Research

characteristic: INSDC first public

text: live

text: Generic

text: Adult

text: marrow

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: Ludwig Institute for Cancer Research

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Adult

text: ovary

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: Stanford University

text: live

text: Generic

characteristic: NCBI submission model

characteristic: cultivar

text: Equal amounts of total RNA by weight from 4 tissue sources (see below) were pooled, polyA+ RNA isolated, and cDNA synthesized for EcoRI (5') and XhoI (3') directional cloning into lambda Hybrizap vector from Stratagene. Tissue Sources: 1. Whole shoots 3 days after sowing/imbibing in wet soil. 2. Basal 1.5 cm shoots 6 days after sowing - includes yellow portions of developing leaves 1-5, primordia from 6-8, and the vegetative apex. 3. Non-green portions of developing leaves 4-5 and the vegetative apex, including adult leaf primordia, 9 days after sowing. 4. Partially expanded and greening leaves 4-5 at 13 days after sowing.

text: 4 stages from 3-13 days after imbibing

text: E. coli XLOLR

characteristic: lab-host

text: juvenile vegetative shoots

text: Zea mays

text: EcoRI

text: XhoI

text: immature leaf primordium and vegetative meristem

characteristic: tissue-type

text: 949 - Juvenile leaf and shoot cDNA from Steve Moose

text: plasmid


text: Dept. of Biochemistry, Medical College, Tongji University

text: live

text: Generic

characteristic: NCBI submission package

text: adult rat

text: Rattus norvegicus

characteristic: organism

text: female and male

characteristic: sex

text: cerebellum

text: Differentially expressed cDNA library of cerebellum of adult rat


text: The University of Georgia, Department of Plant Biology

text: live

text: Generic

text: The library was made from poly-A RNA in the cloning vector lambda ZAP II. Clones to be sequenced were prepared by mass excision.

characteristic: description

text: Developing preanthesis pannicles

text: Sorghum bicolor

text: XhoI

text: EcoRI

text: Immature pannicle 1 (IP1)

characteristic: title

text: Plasmid

text: pBluescript II SK(-) from Lambda Zap II

characteristic: vector


text: AgResearch

text: live

text: Generic

text: Lactating mammary gland cDNA library derived from a non-pregnant, 2 hour post-milking Jersey cow.

text: E. coli XLOLR

text: Mammary Gland

characteristic: organ

text: Bos taurus

text: EcoRI

text: XhoI

text: Alveolar tissue

text: Lactating Mammary Gland A

text: Phagemid

text: pBK-CMV


text: National Institute of Child Health and Human Development, NIH

characteristic: INSDC center name

text: live

text: Generic

text: RNA was reverse-transcribed to first strand cDNA using SuperScriptII reverse-transcriptase and tagged oligo-dT primer which contains several restriction sites including a NotI site: gactagttctagatcgcgatcgcgaGCGGCCGCccttttttttttttttt. Second strand DNA was synthesized by E. coli DNA polymerase I in combination with E. coli RNAse H and E. coli DNA ligase. Double stranded cDNA was ligated with SalI adapter. These cDNAs were cloned into the SalI/NotI site of pBluescript KS+ and transformed into E. coli Electromax DH10B by electroporation.

text: bud to 10 somite stage embryos

text: Danio rerio

text: NotI

text: SalI

text: pBluescript KS+


text: AgResearch

text: live

text: Generic

text: Mammary gland mRNA derived from a 6 year old Friesian cow (MG914), milked the morning of slaughter.

text: mammary gland

text: Bos taurus

text: alveolar tissue

text: Differential, lactationally active vs lactationally inactive

text: Plasmid


text: Chulalongkorn University

text: live

text: Generic

text: Adult

characteristic: dev-stage

text: E.coli XL-1-Blue MRF'

text: Penaeus monodon

text: hemocytes

text: Shrimp Lambda Zap Express Library

text: Phage

text: Lambda ZAP II


characteristic: External Id

text: University of Maryland

text: live

text: Generic

text: male gametophyte

characteristic: cell type

text: Unicellular haploid male spores (microspores) of the water fern Marsilea vestita were grown for 11 hours until completion of spermiogenesis. Total mRNA was extracted at all stages during spermiogenesis to make a cDNA Lambda Zap Expression Library (Stratagene) [Hart and Wolniak, 1998]. MVU refers to Marsilea vestita unknown and the adjoining number refers to the clone number used during isolation of cDNA clones.

text: all stages

text: Marsilea vestita

text: male

text: male gametophyte

text: Marsilea vestita Lambda Zap Expression Library


text: University of Delaware

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: E.coli EMDH10B

text: Gallus gallus

text: Male and Female

text: fat

text: normalized chicken fat cDNA library


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: cDNA made by oligo-dT priming. Directionally cloned into EcoRI/XhoI sites using the following 5' adaptor: GGCACGAG(G). Library constructed by Ling Hong in the laboratory of Gerald M. Rubin (University of California, Berkeley) using ZAP-cDNA synthesis kit (Stratagene) and Superscript II RT (Life Technologies). Note: this is a NIH_MGC Library.

text: DH10B (phage-resistant)

text: salivary gland

text: Homo sapiens

text: XhoI

text: EcoRI

text: epidermoid carcinoma, cell line

text: plasmid (chloramphenicol resistant, grown at 30C)


text: live

text: Generic

text: DH10B (phage-resistant)

characteristic: lab-host

text: brain

text: Homo sapiens

text: XhoI

text: EcoRI

text: astrocytoma grade IV, cell line


text: Medical Research Council, Mammalian Genetics Unit

text: live

text: Generic

text: Islets of Langerhans

text: Embryonic day 18.5 post-fertilization

text: Mus musculus

characteristic: strain

text: Pancreas

characteristic: tissue-type

text: Mouse embryonic 18.5 day pancreatic islet library


text: live

text: Generic

characteristic: NCBI submission package

text: Islets of Langerhans

text: Embryonic day 17.5 post-fertilization

text: Mus musculus

characteristic: organism

text: Pancreas

text: Mouse embryonic 17.5 day pancreatic islet library


text: live

text: Generic

text: Islets of Langerhans

text: Embryonic day 16.5 post-fertilization

text: Mus musculus

text: Pancreas

text: Mouse embryonic 16.5 day pancreatic islet library


text: University of Colorado at Boulder

text: live

text: Generic

text: SL1-PCR-TOPO based library from L3 worms isolated from hamsters. Ancylostoma ceylanicum cDNA was amplified by PCR, using an SL1-EcoRI primer directed to the 5' end and oligo(dT)-XhoI directed to 3' end. PCR products were gel fractionated, and non-directionally cloned into pCR-TOPO-XL (Invitrogen), following the XL-Topo TA cloning protocol. The cDNA insert can be excised with EcoRI. The library was constructed by Dr. Vadim Kapulkin and Adia Magalska at Department of Infectious Diseasaes, Immunology and Parasitology of Veterinary Faculty at the Warsaw Agricultural University. The Ancylostoma ceylanicum strain used in this work was provided by Dr. Jerzy Behnke at the University of Nottingham.

characteristic: description

text: activated, 3rd stage larvae (L3)

text: Top10F' or DH10B

text: whole organism

text: Ancylostoma ceylanicum

text: female and male

characteristic: sex

text: whole organism

text: Vadim Kapulkin Ancylostoma ceylanicum SL1-PCR sub-library

text: pCR-TOPO-XL


text: Umea University

text: live

text: Generic

text: Populus tremula x Populus tremuloides

text: leaf

text: Populus strain T89 leaves

characteristic: title


text: Washington University School of Medicine

text: live

text: Generic

text: The library was constructed by Brandi Chiapelli and Dr. James McCarter at Washington University, St. Louis. The cDNA was made by using Dynabead oligo-dT priming (Dynal). PCR based library using a modified protocol from the SMART PCR cDNA Synthesis Kit from Clontech. Directionally cloned into the UDG sites of pAMP1. Nematodes were provided by Dr. Mark Viney of Bristol, UK.

text: Strongyloides ratti

text: Strongyloides ratti L2 pAMP1 v1 Chiapelli McCarter

text: plasmid

text: pAMP1 (Gibco)

characteristic: vector


characteristic: INSDC center name

text: live

text: Generic

text: Strongyloides ratti

text: Strongyloides ratti L3 adult pAMP1 v1 Chiapelli McCarter

text: plasmid

text: pAMP1 (Gibco)


text: Adelaide University

text: live

text: Generic

text: Galleria mellonella pupal epidermis

text: 4 hours after pupariation

characteristic: dev-stage

text: Galleria mellonella

text: male/female

text: epidermis

text: Galleria mellonella epidermis lambda-ZAP expression library

text: Phage

text: pBluescript


characteristic: External Id

text: Adelaide University

text: live

text: Generic

text: Galleria mellonella final instar larvae

text: Galleria mellonella

text: male/female

text: whole larvae

text: Galleria mellonella larvae Lambda-ZAP expression library

text: Phage

text: pBluescript


text: Adelaide University

text: live

text: Generic

text: Galleria mellonella hemocytes/fatbody from final instar larvae

text: Galleria mellonella

text: male/female

text: hemocytes/fatbody

text: Galleria mellonella hemocyte and fat body Lambda-ZAP expression library

text: Phage

text: pBluescript


text: Vet. Institute

text: live

text: Generic

text: cDNA fragment was found and cloned from the differential displayed genes in early embryos of housefly pro- and post microinjection of targeting gene

text: early embryo

text: Musca domestica

text: Musca domestica early embryo


text: University of Alabama

text: live

text: Generic

text: cDNA clones were selected using a combination of Suppressive subtraction hybridization derived probes and a cDNA library of L. bicolor, prepared from pooled RNA of interaction time points ranging from 6-72 h. Differential expression of these cDNAs was confirmed through screening a membrane array of the cDNAs with exponential probes prepared from control RNA obtained from free living L. bicolor and pooled interaction RNA.

text: Laccaria bicolor

text: Preinfection stage symbiosis-regulated cDNAs from L. bicolor x P. resinosa


text: CSIRO Livestock Industries

text: live

text: Generic

text: Sheep mid flank skin poly-A+ selected RNA was reverse transcribed with MMLV RT. First strand synthesis initiated with XhoI oligo dT linker (GAGAGAGAGAGAGAGAGAGAACTAGTCTCGAGT18). Second strand synthesis performed using RNaseH and DNA polymerase I.

text: adult

text: SURE cells

text: skin

characteristic: organ

text: Ovis aries

text: EcoRI

text: XhoI

text: male

text: Merino

text: Sheep Lambda Zap II Library

text: phagemid

text: pBluescript SK-


text: University of Chicago Medical Center

characteristic: INSDC first public

text: live

text: Generic

text: CD34+ stem/progenitor cells

characteristic: cell type

text: 3'ESTs converted from the SAGE tag sequences using GLGI method

text: Homo sapiens

text: Bone marrow

text: cDNA library of human CD 34+ stem/progenitor cells


text: Lawrence Berkeley National Lab

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Library was kindly generated by Piero Carninci at the RIKEN. The library was normalized and excised using Cre recombinase. Plasmid cDNA library.

text: Adult

text: DH5-alpha TonA

text: head

text: Drosophila melanogaster

text: XhoI

text: BamHI

text: male and female

text: RH Drosophila melanogaster normalized Head pFlc-1


text: Lawrence Berkeley National Lab

text: live

text: Generic

characteristic: NCBI submission model

text: 0-24 hours mixed stage embryonic

text: DH5-alpha TonA

characteristic: lab-host

text: embryo

text: Drosophila melanogaster

text: XhoI

text: BamHI

text: male and female

text: RE Drosophila melanogaster normalized Embryo pFlc-1


text: Crop and Food Research

text: live

text: Generic

text: Pukekohe Longkeeper

characteristic: cultivar

text: A. cepa sterilized seeds were germinated for 72h at 25 C before being transferred to hydroponic solution (Randle et al., 1995). The hydroponic solution was changed at 2 weekly intervals and after 8 weeks half of the plants were subjected to a 0% sulfur hydroponic solution for 48 hrs. RNA was extracted from root tissue of plants from both treatments. cDNAs differentially expressed under sulfur starvation were produced from polyA+ enriched RNA using the Clontech PCR-Select cDNA Subtraction Kit. The cDNAs were ligated into pGEM-T Easy vector from Promega and clones were randomly chosen for sequencing.

text: Before bulbing

text: DH5 alpha

text: Root

text: Allium cepa

text: Sulfur-deprived onion root library

text: Plasmid

text: pGEM-T Easy


text: CNRS, Institut Fessard

text: live

text: Generic

characteristic: NCBI submission package

text: Oryzias latipes

text: Orange-red

characteristic: strain

text: anterior head

characteristic: tissue-type

text: Oryzias latipes anterior head Orange-red 30-31


text: Eberhard-Karls-Universitaet

text: live

text: Generic

text: Populus tremula x P. tremuloides/Amanita muscaria mixed EST library

characteristic: organism


text: C.N.R.S., Institut des Sciences du Vegetal

text: live

text: Generic

text: Jemalong

text: Medicago truncatula

text: Medicago truncatula Jemalong library (Ratet P)

characteristic: title


text: Okayama University, Barley Germplasm Center

text: live

text: Generic

text: Akashinriki

text: vegetative stage

text: Hordeum vulgare subsp. vulgare

text: leaves

text: K. Sato unpublished cDNA library: Hordeum vulgare subsp. vulgare leaves vegetative stage


text: live

text: Generic

text: Haruna Nijo

text: adult, heading stage

text: Hordeum vulgare subsp. vulgare

text: top three leaves

text: K. Sato unpublished cDNA library: Hordeum vulgare subsp. vulgare top three leaves adult, heading stage


text: live

text: Generic

text: Haruna Nijo

text: germination

text: Hordeum vulgare subsp. vulgare

text: shoots

text: K. Sato unpublished cDNA library: Hordeum vulgare subsp. vulgare shoots germination


text: live

text: Generic

text: adult, heading stage

text: Hordeum vulgare subsp. spontaneum

text: top three leaves

text: K. Sato unpublished cDNA library: Hordeum vulgare subsp. spontaneum top three leaves adult, heading stage


text: live

text: Generic

text: Haruna Nijo

text: second leaf stage

text: Hordeum vulgare subsp. vulgare

text: seedling leaves

text: K. Sato unpublished cDNA library: Hordeum vulgare subsp. vulgare seedling leaves second leaf stage


text: Georgetown University

text: live

text: Generic

text: cancer

text: Homo sapiens

text: breast

text: Homo sapiens breast cancer


text: Institut National Polytechnique de Toulouse - Ecole National Superieure Agronomique de Toulouse

text: live

text: Generic

text: protoplast

text: Emil

text: Helianthus annuus

text: Helianthus annuus cv. Emil protoplast


text: live

text: Generic

text: Helianthus annuus

text: Helianthus annuus library (Tamborindeguy C)


text: University of Notre Dame

text: live

text: Generic

text: cDNA fragment was obtained by differential display while comparing Plasmodium gallinaceum-infected midguts

characteristic: description

text: adult female

text: Aedes aegypti

text: female

characteristic: sex

text: midgut

text: bulk 1 F2 midgut


text: The Netherlands Cancer Institute

characteristic: INSDC center name

text: live

text: Generic

text: Homo sapiens

text: Homo sapiens library (Geerts D)


characteristic: External Id

text: Southern Cross University

text: live

text: Generic

text: Melaleuca alternifolia

text: Melaleuca alternifolia (Cheel) mRNA


text: Nevada Department of Agriculture

text: live

text: Generic

text: mixed

text: Tissues and cells are derived from a porcine model for implant-associated infection using 1000 cfu of Staphylococcus aureus in a tibial transection, reduced and internally fixed with a dynamic compression plate. NOTE: The sequences contain a 'cDNA adapter' between the EcoRI site and the start of the EST. The adapter sequence is 'AATTCGGCACGAG'.

text: control, 5 month old castrated male

characteristic: dev-stage

text: SOLR

text: Sus scrofa

text: Eco RI

text: XhoI

text: crossbreed

text: Lymph node

text: Porcine Lymph node cDNA library

text: Plasmid

text: pBSK

characteristic: vector


text: Nevada Department of Agriculture

characteristic: INSDC first public

text: live

text: Generic

text: mixed

characteristic: cell type

text: control, 5 month old castrated male

text: SOLR

text: Sus scrofa

text: Eco RI

text: XhoI

text: crossbreed

text: Bone Marrow

text: Porcine Bone Marrow cDNA library

text: Plasmid

text: pBSK


text: Nevada Department of Agriculture

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: mixed

text: control, 5 month old castrated male

text: SOLR

text: Sus scrofa

text: Eco RI

text: XhoI

text: crossbreed

text: Peripheral Blood Cell

text: Porcine Peripheral Blood Cell cDNA library

text: Plasmid

text: pBSK


text: Nevada Department of Agriculture

text: live

text: Generic

characteristic: NCBI submission model

text: mixed

text: control, 5 month old castrated male

text: SOLR

characteristic: lab-host

text: Sus scrofa

text: Eco RI

text: XhoI

text: crossbreed

text: Spleen

text: Porcine Spleen cDNA library

text: Plasmid

text: pBSK


text: Nevada Department of Agriculture

text: live

text: Generic

characteristic: NCBI submission package

text: mixed

text: control, 5 month old castrated male

text: SOLR

text: Sus scrofa

text: Eco RI

text: XhoI

text: crossbreed

text: Thymus

text: Porcine Thymus cDNA library

text: Plasmid

text: pBSK


text: Nevada Department of Agriculture

text: live

text: Generic

text: mixed

text: 28 days post infection, 5 month old castrated male

text: SOLR

text: Sus scrofa

text: Eco RI

text: XhoI

text: crossbreed

characteristic: strain

text: Lymph node

text: Infected Porcine Lymph node cDNA library

text: Plasmid

text: pBSK


text: Nevada Department of Agriculture

text: live

text: Generic

text: mixed

text: SOLR

text: Sus scrofa

text: Eco RI

text: XhoI

text: crossbreed

text: Bone Marrow

characteristic: tissue-type

text: Infected Porcine Bone Marrow cDNA library

text: Plasmid

text: pBSK


text: Nevada Department of Agriculture

text: live

text: Generic

text: mixed

text: SOLR

text: Sus scrofa

characteristic: organism

text: Eco RI

text: XhoI

text: crossbreed

text: Peripheral Blood Cell

text: Infected Porcine Peripheral Blood Cell cDNA library

text: Plasmid

text: pBSK


text: Nevada Department of Agriculture

text: live

text: Generic

text: mixed

text: SOLR

text: Sus scrofa

text: Eco RI

text: XhoI

text: crossbreed

text: Spleen

text: Infected Porcine Spleen cDNA library

characteristic: title

text: Plasmid

text: pBSK


text: Nevada Department of Agriculture

text: live

text: Generic

text: mixed

characteristic: description

text: SOLR

text: Sus scrofa

text: Eco RI

text: XhoI

text: crossbreed

text: Thymus

text: Infected Porcine Thymus cDNA library

text: Plasmid

text: pBSK


text: Nevada Department of Agriculture

text: live

text: Generic

text: mixed

text: control, 5 month old castrated male

text: SOLR

text: Sus scrofa

text: Eco RI

text: XhoI

text: crossbreed

text: Lymph node

text: Porcine Lymph node cDNA library, Cot 5

text: Plasmid

text: pBSK


text: Nevada Department of Agriculture

text: live

text: Generic

text: mixed

text: control, 5 month old castrated male

text: SOLR

text: Sus scrofa

text: Eco RI

text: XhoI

text: crossbreed

text: Lymph node

text: Porcine Lymph node cDNA library, Cot 10

text: Plasmid

text: pBSK


text: Nevada Department of Agriculture

text: live

text: Generic

text: mixed

text: control, 5 month old castrated male

text: SOLR

text: Sus scrofa

text: Eco RI

text: XhoI

text: crossbreed

text: Lymph node

text: Porcine Lymph node cDNA library, Cot 30

text: Plasmid

text: pBSK


text: Nevada Department of Agriculture

text: live

text: Generic

text: mixed

text: control, 5 month old castrated male

text: SOLR

text: Sus scrofa

text: Eco RI

text: XhoI

text: crossbreed

text: Peripheral Blood Cell

text: Porcine Peripheral Blood Cell cDNA library, Cot 05

text: Plasmid

text: pBSK


text: College of Agriculture, Biotechnology and Natural Resources, University of Nevada, Reno

text: live

text: Generic

text: mixed

text: control, 5 month old castrated male

text: SOLR

text: Sus scrofa

text: Eco RI

text: XhoI

text: crossbreed

text: Peripheral Blood Cell

text: Porcine Peripheral Blood Cell cDNA library, Cot 10

text: Plasmid

text: pBSK


text: Nevada Department of Agriculture

text: live

text: Generic

text: mixed

text: control, 5 month old castrated male

text: SOLR

text: Sus scrofa

text: Eco RI

text: XhoI

text: crossbreed

text: Peripheral Blood Cell

text: Porcine Peripheral Blood Cell cDNA library, Cot 20

text: Plasmid

text: pBSK


text: Nevada Department of Agriculture

text: live

text: Generic

text: mixed

text: control, 5 month old castrated male

text: SOLR

text: Sus scrofa

text: Eco RI

text: XhoI

text: crossbreed

text: Peripheral Blood Cell

text: Porcine Peripheral Blood Cell cDNA library, Cot 30

text: Plasmid

text: pBSK


text: Nevada Department of Agriculture

text: live

text: Generic

text: mixed

text: control, 5 month old castrated male

text: SOLR

text: Sus scrofa

text: Eco RI

text: XhoI

text: crossbreed

text: Spleen

text: Porcine Spleen cDNA library, Cot 05

text: Plasmid

text: pBSK


text: Nevada Department of Agriculture

text: live

text: Generic

text: mixed

text: control, 5 month old castrated male

text: SOLR

text: Sus scrofa

text: Eco RI

text: XhoI

text: crossbreed

text: Spleen

text: Porcine Spleen cDNA library, Cot 10

text: Plasmid

text: pBSK


text: Nevada Department of Agriculture

text: live

text: Generic

text: mixed

text: control, 5 month old castrated male

text: SOLR

text: Sus scrofa

text: Eco RI

text: XhoI

text: crossbreed

text: Spleen

text: Porcine Spleen cDNA library, Cot 20

text: Plasmid

text: pBSK


text: Nevada Department of Agriculture

text: live

text: Generic

text: mixed

text: control, 5 month old castrated male

text: SOLR

text: Sus scrofa

text: Eco RI

text: XhoI

text: crossbreed

text: Spleen

text: Porcine Spleen cDNA library, Mini, Cot 30

text: Plasmid

text: pBSK


text: live

text: Generic

text: mixed

text: control, 5 month old castrated male

text: SOLR

text: Sus scrofa

text: Eco RI

text: XhoI

text: crossbreed

text: Lymph node

text: Porcine Lymph node cDNA library, Mini, Cot 30

text: Plasmid

text: pBSK


text: live

text: Generic

text: mixed

text: control, 5 month old castrated male

text: SOLR

text: Sus scrofa

text: Eco RI

text: XhoI

text: crossbreed

text: Peripheral Blood Cell

text: Porcine Peripheral Blood Cell cDNA library, Mini, Cot 05

text: Plasmid

text: pBSK


text: live

text: Generic

text: mixed

text: control, 5 month old castrated male

text: SOLR

text: Sus scrofa

text: Eco RI

text: XhoI

text: crossbreed

text: Spleen

text: Porcine Spleen cDNA library, Cot 30

text: Plasmid

text: pBSK


text: Southern Illinois University at Carbondale

text: live

text: Generic

text: Forrest

characteristic: cultivar

text: Forrest roots were inoculated with Fusarium solani f. sp. glycinae and samples were collected after 14 days of inoculation. Total RNA was used for cDNA synthesis using SMART PCR cDNA synthesis Kit (CLONTECH, CA). CLONTECH PCR-Select Subtraction system was used to enrich rare transcripts expressed in Forrest roots under inoculation by F. solani and cloned in pGEM vector. ESTs were sequenced using M13 universal forward or reverse primer.

text: 14 days after inoculation

text: Root

characteristic: organ

text: Glycine max

text: Forrest infected Subtraction Library

text: Plasmid

text: pGEM


characteristic: External Id

characteristic: INSDC center name

text: live

text: Generic

text: mixed

text: control, 5 month old castrated male

text: SOLR

text: Sus scrofa

text: Eco RI

text: XhoI

text: crossbreed

text: Lymph node

text: Porcine Lymph node cDNA library, Cot 20

text: Plasmid

text: pBSK


text: Airforce General Hospital

text: live

text: Generic

text: heart

text: Rattus norvegicus

text: male

characteristic: sex

text: Sprague-Dawley

text: heart

text: SSH cDNA library


text: Umea University

characteristic: INSDC first public

text: live

text: Generic

text: flower

text: Populus trichocarpa

text: floral buds

text: Populus flower cDNA library


text: Department of Plant Physiology

text: live

text: Generic

text: leaf

text: Populus tremula x Populus tremuloides

text: Populus leaf cDNA library


text: Royal Institute of Technology

text: live

text: Generic

text: cambium

text: Populus tremula x Populus tremuloides

text: Populus cambium cDNA library


text: Zhongshan University

text: live

text: Generic

text: stroma

characteristic: cell type

text: fetal

characteristic: dev-stage

text: Homo sapiens

text: bone marrow

text: Homo sapiens fetal bone marrow


text: Anhui Medical University

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: schistosomula day 17 post-infection

text: E.coli XL1-blue

text: Schistosoma japonicum

text: EcoRI

text: XhoI

text: mixed

text: Chinese mainland

text: Schistosoma japonicum schistosomula Lambda TriplEx2 cDNA Express library

text: Lambda TriplEx2

characteristic: vector


text: Southwest Hospital, Third Military Medical University

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: dermal papilla cell

text: cDNA was made by SMART PCR cDNA synthesis kit (Clontech). Subtractive library was established using subtractive hybridization technique in order to clone the aggregation related genes fragments of dermal papilla cells.

text: primary culture

text: skin

text: Homo sapiens

text: male

text: scalp

text: Human Cultured Dermal Papilla Cell cDNA Library

text: Cosmid

text: pUCm


text: Tokyo University of Marine Science and Technology, Graduate School of Marine Science and Technology

text: live

text: Generic

text: hemocytes

text: adult

text: Penaeus japonicus

text: Marsupenaeus japonicus adult

text: lambda ZAP II


text: Genox Research, Inc.

text: live

text: Generic

text: T-cell

text: Homo sapiens

text: Homo sapiens T-cell library (Sugita Y)


text: Genox Research, Inc.

text: live

text: Generic

text: E-cell

text: Homo sapiens

text: Homo sapiens E-cell library (Sugita Y)


text: Genox Research, Inc.

text: live

text: Generic

text: Mast cell

text: Homo sapiens

text: Homo sapiens Mast cell library (Sugita Y)


text: Genox Research, Inc.

text: live

text: Generic

text: monocyte

text: Homo sapiens

text: Homo sapiens monocyte library (Sugita Y)


text: Universiteit Gent

text: live

text: Generic

text: Sesbania rostrata

text: Sesbania rostrata mRNA


text: Washington University School of Medicine

text: live

text: Generic

text: SL1-Oligo(dT) PCR-based library. Strongyloides_ratti_L2 cDNA PCR products of size >400 nucleotides containing SL1 on the 5' end and oligo(dT) on the 3' end were non-directionally cloned into pCRII-TOPO (Invitrogen) following the Topo TA cloning protocol. The cDNA insert can be excised by digestion with EcoRI. The library was constructed by Brandi Chiapelli and Dr. James McCarter at Washington University, St. Louis. Nematodes were provided by Dr. Mark Viney of Bristol, UK.

characteristic: lab-host

text: Strongyloides ratti

text: Strongyloides ratti L2 SL1 TOPO v2

text: plasmid (amp. or kan. resistant)

text: pCRII-TOPO


text: Univ. of Vienna

text: live

text: Generic

characteristic: cell-line

text: cDNA isolated from a subclone derived from A431 (ATCC) overexpressing the small heat shock protein hsp27

text: Homo sapiens

characteristic: organism

text: Human cell line A431 subclone overexpressing hsp27


text: Airforce General Hospital

text: live

text: Generic

text: brain

text: Rattus norvegicus

text: male

text: Sprague-Dawley

characteristic: strain

text: brain

characteristic: tissue-type

text: SSH brain cDNA library

characteristic: title


characteristic: External Id

text: live

text: Generic

text: Differential display of mRNA technique was used to detect host gene expression changes during the compatible interaction between soy bean and pathogen (Heterodera glycines). Total RNA extracted from pathogen infected and un-infected soybean roots five days, ten days and fifteen days after inoculation. After RT-PCR reaction, six clones showed hybridization intensity differences in the slot blot assay.

characteristic: description

text: Glycine max

text: cDNA sequences from soybean roots induced by pathogen (Heterodera glycines)


text: Dana Farber Cancer Institute

characteristic: INSDC center name

text: live

text: Generic

text: The AD-wrmcDNA library was generated with poly(A)+ RNA isolated from both hermaphrodite and male N2 worms of all larval stages, embryos, adults and dauers and the subsequent generation of cDNAs by poly(A) priming. The cDNAs were cloned into pPC86

text: mixed stage

text: Caenorhabditis elegans

text: Hermaphrodite and male

characteristic: sex

text: whole animal

text: AD-wrmcDNA


text: The Institute for Genomic Research (TIGR; www.tigr.org)

characteristic: INSDC first public

text: live

text: Generic

text: Bintje

characteristic: cultivar

text: Tissue supplied by Christian Bachem and Richard Visser (Department of Plant Breeding, Wageningen University, The Netherlands). The cSTA libraries will attempt to capture the induction and initiation/initial growth of the tuber in an in vitro system as described in Bachem et al. (Plant Journal, 1996). Small microtubers develop from axillary buds attached to stem explants when placed on a high sucrose medium (10%). Visible morphological changes occur synchronously at day five in the axillary buds. The first library, cSTA (1-20) consists of axillary buds harvested on days 1-3. This targets those genes involved in induction of the microtubers. The following libraries, cSTA (21-40) and cSTA (41-60), capture genes involved in tuber initiation and outgrowth. This library is noted as P3 in Tanksley lab notebooks.

text: 7, 8 and 10 days

text: SOLR

text: Solanum tuberosum

text: EcoRI

text: XhoI

text: axillary buds of stem explants; growing sink-tubers

text: cSTE

text: pBluescript SK(-)


text: Yale University School of Medicine

characteristic: INSDC last update

text: live

text: Generic

text: LRH library was constructed from cDNA of primary bone marrow cells depleted of lineage-committed cells and enriched for primitive cells by FACS sorting for cells with low level staining with rhodamine123 and Hoechst 33342 dyes. cDNA from 5000 cells derived from 30 mice, 5000 cells were were directionally cloned into SalI-EagI restriction site of the ( ZipLox (Gibco BRL). The ligated cDNA fragments were transformed into DH10B host cells. The original library had an initial plating complexity of 1.44X107 clones.

text: Adult

characteristic: dev-stage

text: DH10B (phage-resistant)

text: Mus musculus

text: SalI site

text: EagI site

text: Female

text: BALB/c

text: Primary sorted bone marrow cells

text: LRH (Lin Rhodamine and Hoechst dye)


text: Yale University School of Medicine

text: live

characteristic: INSDC status

text: Generic

text: Murine hematopoietic progenitor

characteristic: cell type

text: EML and EPRO cells

text: EEE library was differentiated from cDNA subtracted by EML(0h) cultured in SCF only, EML (6h) treated with SCF (100ng/ml), Interleukin-3 (30ng/ml) and all-trans retinoic acid (ATRA) and EPRO cultured in SCF/IL-3/ATRA for 72h with 20ng/ml murine GM-CSF cells. EML (0h), EML (6h), and EPRO cDNA were directionally cloned into EcoR1-XhoI restriction site of the (ZAP-express (Stratagene). The ligated cDNA fragments were transformed into DH10B host cells.

text: DH10B (phage-resistant)

text: Mus musculus

text: XhoI

text: EEE (0hEML, 6hEML and EPRO)

text: pBK-CMV

characteristic: vector


text: Clemson University

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: TA496, E6203

text: Suspension cultures of L.esculentum E6203 were grown in Murashige and Skoog based medium, supplemented with 15% coconut milk (filter sterilized and added after autoclaving), 2% saccharose, and 1mg/ml 2,4D (pH5.8). Fresh medium was added every 7 days, and cultures were grown at 25 C, with 12hrs of light and continuous shaking.

text: SOLR

characteristic: lab-host

text: Solanum lycopersicum

text: EcoRI

text: XhoI

text: suspension cultures

text: cTOS

text: pBluescript SK(-)


text: Fox Chase Cancer Center

text: live

text: Generic

text: cDNAs were synthesized by reverse transcription of mRNAs purified from the liver and a hepatocellular carcinoma (HCC) of a woodchuck (marmota monax) chronically infected with woodchuck hepatitis virus. cDNAs were digested with RsaI and ligated to one of two different adaptors. HCC enriched cDNA was then generated using the Clontech PCR Select cDNA subtraction kit. Amplicons with non-identical adaptors at each end were selectively amplified by PCR, using primers specific to the adaptors, and the resulting amplicons were ligated into pGEM T easy (Promega).

text: Liver

characteristic: organ

text: Marmota monax

characteristic: organism

text: Hepatocellular carcinoma

text: hepatocellular carcinoma expression library


text: University of Nebraska, Lincoln

text: live

text: Generic

text: The UNL-P-F2 library represents porcine ovarian follicles, ranging between 2.0 to 10.0 mm in diameter, collected during 7 days of the follicular phase of the pig estrous cycle. This library was derived from UNL-P-F2. The tag is a string of 5-6 nucleotides present between the Not I site and the oligo-dT track. The library was constructed as described by Bonaldo, Lennon and Soares, Genome Research 6: 791-806, 1996.

text: ADULT

text: DH10B (Life Technologies)

text: Sus scrofa

text: Not I

text: Eco RI

text: University of Nebraska, Lincoln Swine Selection Lines

characteristic: strain

characteristic: title

text: Phagemid


characteristic: External Id

text: University of Nebraska, Lincoln

text: live

text: Generic

text: The UNL-P-FN library is a normalized library representing porcine ovarian follicles, ranging between 2.0 to 10.0 mm in diameter, collected during 7 days of the follicular phase of the pig estrous cycle. This library was derived from the library UNL-P-F2. The tag is a string of 5-6 nucleotides present between the Not I site and the oligo-dT track. The library was constructed as described by Bonaldo, Lennon and Soares, Genome Research 6: 791-806, 1996.

characteristic: description

text: ADULT

text: DH10B (Life Technologies)

text: Sus scrofa

text: Not I

text: Eco RI

text: UNL-P-FN

text: Phagemid


text: Shanghai Tiedao University Medical School

characteristic: INSDC center name

text: live

text: Generic

text: grown up

text: Rattus norvegicus

text: male

characteristic: sex

text: brain

characteristic: tissue-type

text: Differentially expressed cDNA library of rat brain with injection of A beta peptide 1-40


text: IRTA

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: synonym: Prunus amygdalus

text: Prunus dulcis

text: Almond Lambda Zap II cDNA Library


text: IRTA

text: live

characteristic: INSDC status

text: Generic

text: Prunus persica

text: Peach cDNA Library


text: IRTA

text: live

text: Generic

text: Prunus avium

text: Cherry cDNA Library


text: IRTA

text: live

text: Generic

text: m27 rootstock

text: Malus domestica

text: Apple cDNA Library


text: The Institute for Genomic Research

text: live

text: Generic

text: This library was constructed by directed cloning into the EcoRI-XhoI sites of pAD-GAL4.Estimated Average insert size approximately 1.2kb based on 15 clones, estimated titer of excised phagemid library = 3.78 x 106 cfu/ml

text: maternal

characteristic: dev-stage

text: XL1-Blue MRF'

text: Danio rerio

text: whole body

text: zebrafish T0304, Steve Ekker

text: phagemid


text: The Institute for Genomic Research

text: live

text: Generic

text: early gastrulation

text: XL1-Blue MRF'

text: Danio rerio

text: whole body

text: zebrafish TO297, Steve Ekker

text: phagemid

characteristic: vector


text: The Institute for Genomic Research

text: live

text: Generic

characteristic: NCBI submission model

text: Post segmentation (somatogenesis)

text: XL1-Blue MRF'

text: Danio rerio

text: whole body

text: zebrafish T0315, Steve Ekker

text: phagemid


text: The Sanger Institute The Wellcome Trust Genome Campus

text: live

text: Generic

characteristic: NCBI submission package

text: Echinococcus granulosus is a cestode parasite of dogs (definitive host) and various domestic and wild animals as well as humans (intermediate hosts). The library was constructed from the wall (germinative and laminated layers) obtained from a hydatid cyst present in the lungs of a naturally infected Uruguayan bovine. cDNA was prepared by RT-PCR and directionally cloned in a plasmid vector. RT was primed with a tagged oligo-dT (NotI primer-adapter). This tag was then used to prime cDNA amplification together with a primer directed against an oligonucleotide previously ligated to the originally capped RNA population. cDNA was ligated to SalI adapters, digested with NotI, and cloned into pSPORT 1 cut with SalI and NotI.

text: larva (metacestode)

text: Echinococcus granulosus

text: SalI (5'end)

text: NotI (3'end)

text: Echinococcus granulosus germinative layer (full length enriched)


text: Centro de Biotecnologia

text: live

text: Generic

text: Echinococcus granulosus is a cestode parasite of dogs (definitive host) and various domestic and wild animals as well as humans (intermediate hosts). The library was constructed from the wall (germinative and laminated layers) obtained from a hydatid cyst present in the lungs of a naturally infected Uruguayan bovine. cDNA was prepared by RT-PCR and directionally cloned in a plasmid vector. RT was primed with a tagged oligo-dT (NotI primer-adapter). This tag was then used to prime cDNA amplification together with a primer for the recently characterised E. granulosus spliced leader sequence (Brehm et al, J Biol Chem 275: 38311-8, 2000). cDNA was ligated to SalI adapters, digested with NotI, and cloned into pSPORT 1 cut with SalI and NotI.

text: larva (metacestode)

text: Echinococcus granulosus

text: SalI (5'end)

text: NotI (3'end)

text: Echinococcus granulosus germinative layer (trans-spliced)


text: live

text: Generic

text: Echinococcus granulosus is a cestode parasite of dogs (definitive host) and various domestic and wild animals as well as humans (intermediate hosts). The library was constructed from protoscoleces obtained from hydatid cysts present in the lungs of naturally infected Uruguayan bovines. cDNA was prepared by RT-PCR and directionally cloned in a plasmid vector. RT was primed with a tagged oligo-dT (NotI primer-adapter). This tag was then used to prime cDNA amplification together with a primer directed against an oligonucleotide previously ligated to the originally capped RNA population. cDNA was ligated to SalI adapters, digested with NotI, and cloned into pSPORT 1 cut with SalI and NotI.

text: larva (protoscolex)

text: Echinococcus granulosus

text: SalI (5'end)

text: NotI (3'end)

text: Echinococcus granulosus protoscolex (full length enriched)


text: live

text: Generic

text: Echinococcus granulosus is a cestode parasite of dogs (definitive host) and various domestic and wild animals as well as humans (intermediate hosts). The library was constructed from protoscoleces obtained from hydatid cysts present in the lungs of naturally infected Uruguayan bovines. cDNA was prepared by RT-PCR and directionally cloned in a plasmid vector. RT was primed with a tagged oligo-dT (NotI primer-adapter). This tag was then used to prime cDNA amplification together with a primer for the recently characterised E. granulosus spliced leader sequence (Brehm et al, J Biol Chem 275: 38311-8, 2000). cDNA was ligated to SalI adapters, digested with NotI, and cloned into pSPORT 1 cut with SalI and NotI.

text: larva (protoscolex)

text: Echinococcus granulosus

text: SalI (5'end)

text: NotI (3'end)

text: Echinococcus granulosus protoscolex (trans-spliced)


text: live

text: Generic

text: Echinococcus granulosus is a cestode parasite of dogs (definitive host) and various domestic and wild animals as well as humans (intermediate hosts). The library was constructed from protoscoleces obtained from hydatid cysts present in the lungs of naturally infected Uruguayan bovines. Prior to RNA extraction, protoscoleces were treated with pepsin (0.5 mg/ml in Hanks' solution pH 2.0, for 3 h at 37C). cDNA was prepared by RT-PCR and directionally cloned in a plasmid vector. RT was primed with a tagged oligo-dT (NotI primer-adapter). This tag was then used to prime cDNA amplification together with a primer directed against an oligonucleotide previously ligated to the originally capped RNA population. cDNA was ligated to SalI adapters, digested with NotI, and cloned into pSPORT 1 cut with SalI and NotI.

text: larva (protoscolex)

text: Echinococcus granulosus

text: SalI (5'end)

text: NotI (3'end)

text: Echinococcus granulosus pepsin-treated protoscolex (full length enriched)


text: Centro de Biotecnologia

text: live

text: Generic

text: Echinoccocus granulosus is a cestode parasite of dogs (definitive host) and various domestic and wild animals as well as humans (intermediate hosts). The library was constructed from protoscoleces obtained from hydatid cysts present in the lungs of naturally infected Uruguayan bovines. Prior to RNA extraction, protoscoleces were treated with pepsin (0.5 mg/ml in Hanks' solution pH 2.0, for 3 h at 37C). cDNA was prepared by RT-PCR and directionally cloned in a plasmid vector. RT was primed with a tagged oligo-dT (NotI primer-adapter). This tag was then used to prime cDNA amplification together with a primer for the recently characterised E. granulosus spliced leader sequence (Brehm et al, J Biol Chem 275: 38311-8, 2000). cDNA was ligated to SalI adapters, digested with NotI, and cloned into pSPORT 1 cut with SalI and NotI.

text: larva (protoscolex)

text: Echinococcus granulosus

text: SalI (5'end)

text: NotI (3'end)

text: Echinococcus granulosus pepsin-treated protoscolex (trans-spliced)


text: Institut National de la Recherche Agronomique

text: live

text: Generic

characteristic: cultivar

text: Suppression Subtractive Hybridization of Medicago truncatula and Glomus mosseae

text: 3 weeks

text: Medicago truncatula

text: mycorrhized roots

text: Medicago truncatula mycorrhized roots 3 weeks


text: University H. Poincare - Nancy 1

text: live

text: Generic

text: monokaryotic

text: Hebeloma cylindrosporum

text: mycelium

text: Hebeloma cylindrosporum cDNAs

text: plasmid

text: pGEM-T


text: Anhui Medical University

text: live

text: Generic

text: schistosomula day 15 post-infection

text: E.coli XL1-blue

characteristic: lab-host

text: Schistosoma japonicum

text: EcoRI

text: XhoI

text: mixed

text: Chinese mainland

text: Schistosoma japonicum schistosomula Lambda TriplEx2 cDNA Express library day 15 post-infection

text: Lambda TriplEx2


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: cDNA made by oligo-dT priming. Directionally cloned into EcoRI/XhoI sites using the following 5' adaptor: GGCACGAG(G). Size-selected >500bp for average insert size 1.8kb. Library constructed by Ling Hong in the laboratory of Gerald M. Rubin (University of California, Berkeley) using ZAP-cDNA synthesis kit (Stratagene) and Superscript II RT (Life Technologies). Note: this is a NIH_MGC Library.

text: DH10B (phage-resistant)

text: lymph

characteristic: organ

text: Homo sapiens

characteristic: organism

text: XhoI

text: EcoRI

text: lymphoma, cell line

text: plasmid (chloramphenicol resistant, grown at 30C)


text: live

text: Generic

text: DH10B (phage-resistant)

text: liver

text: Homo sapiens

text: XhoI

text: EcoRI

text: hepatocellular carcinoma, cell line

characteristic: title


characteristic: External Id

text: Institute of Molecular Biology, The First Military Medical University

text: live

text: Generic

text: leukemia cell

characteristic: cell type

text: k562 cell line

characteristic: cell-line

text: Culturing four days induced by hydroxyurea

text: Homo sapiens

text: k562 cell cDNA Library

text: pMD18-T vector


text: National Institute of Genetics

characteristic: INSDC center name

text: live

text: Generic

text: Caenorhabditis elegans

text: Hermaphrodite

characteristic: sex

characteristic: strain

text: whole animal

text: unpublished oligo-capped cDNA library, stage L2


text: National Institute of Genetics

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Caenorhabditis elegans

text: Hermaphrodite

text: whole animal

characteristic: tissue-type

text: unpublished oligo-capped cDNA library, stage L1


text: National Institute of Genetics

text: live

characteristic: INSDC status

text: Generic

characteristic: dev-stage

text: Caenorhabditis elegans

text: Hermaphrodite

text: whole animal

text: unpublished oligo-capped cDNA library, stage L4


text: Kansas State Universtiy

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: 5th Instar larva

text: Manduca sexta

text: EcoRI

text: XhoI

text: Fat Body

text: Subtracted larval fat body library, bacteria-induced genes

text: Plasmid

text: pGEM-T Vector

characteristic: vector


text: The Samuel Roberts Noble Foundation

text: live

text: Generic

text: cDNA was prepared from polyA+ enriched, pooled samples of equivalent amounts of total RNA from very young, developing, fully-opened flowers and flowers transitioning into pods. The cDNA was directionally ligated into the Uni-Zap XR vector (Stratagene) and packaged using the Gigapack III Gold packaging extracts. Phagemids containing cDNA inserts were in vivo excised from the recombinant Uni-ZAP XR vector using ExAssist helper phage and the E. coli strain XL1-Blue MRF' (Stratagene). Excised plasmids were plated using SOLR cells.

characteristic: description

text: Developmentally pooled. Contains a mixture of very young, developing, fully-opened flowers and flowers in early transition into pods.

text: Medicago truncatula

text: Developing flowers

text: Developing flower

text: Plasmid

text: Lambda Zap


text: The Samuel Roberts Noble Foundation

text: live

text: Generic

text: M. truncatula seeds were acid treated and placed on wet filter papers in petri dishes. Seeds were harvested at 0, 1, 2 and 3 days after acid treatment. cDNA was prepared from polyA+ enriched, pooled samples of equivalent amounts of total RNA from each time point. The cDNA was directionally ligated into the Uni-Zap XR vector (Stratagene) and packaged using the Gigapack III Gold packaging extracts. Phagemids containing cDNA inserts were in vivo excised from the recombinant Uni-ZAP XR vector using ExAssist helper phage and the E. coli strain XL1-Blue MRF' (Stratagene). Excised plasmids were plated using SOLR cells.

text: Medicago truncatula

characteristic: organism

text: germinating seeds

text: Germinating Seed

text: Plasmid

text: Lambda Zap


text: The Samuel Roberts Noble Foundation

text: live

text: Generic

text: Seedlings were exposed either to 100 gy gamma or 0.5, 1, 5, or 10 kJ/m2 UV irradiation. Gamma-irradiated samples were harvested at 6, 12, 24 and 48 hours after treatment. UV-irradiated samples were harvested 24 hours post-treatment. cDNA was prepared from polyA+ enriched, pooled samples of equivalent amounts of total RNA from each sample. The cDNA was directionally ligated into the Uni-Zap XR vector (Stratagene) and packaged using the Gigapack III Gold packaging extracts. Phagemids containing cDNA inserts were in vivo excised from the recombinant Uni-ZAP XR vector using ExAssist helper phage and the E. coli strain XL1-Blue MRF' (Stratagene). Excised plasmids were plated using SOLR cells.

text: seedling

text: Medicago truncatula

text: seedlings

text: Irradiated

characteristic: title

text: Plasmid

text: Lambda Zap


characteristic: External Id

text: Oklahoma State University

text: live

text: Generic

text: Genes expressed differently in feeding vs. unfed ticks isolated by RAP-PCR

text: adult

text: salivary gland

characteristic: organ

text: Amblyomma americanum

text: EcoRI

text: EcoRI

text: male

text: Feeding vs. unfed male Amblyomma americanum

text: plasmid

text: pCRII


text: Ocean University of Qingdao

text: live

text: Generic

text: Mature leafy gametophytes were rinsed extensively with boiled seawater, and cut into small pieces. Single tissue piece was cultivated in liquid Provasoli medium until filamentous sporophyte colonies come out. The most vigorous colony was cultivated and maintained as strains, such as Y9604, and the majority of them are pure genetically. Total RNA was isolated using UNIQ-10 Column Centrifugation Trizol Total RNA Isolation Kit (Sangon Inc., Shanghai, China, Fax: 021-64516369, Tel: 021-64519388). The cDNA was synthesized, amplified and cloned after size selection using cDNA Synthesis and cDNA PCR Library Kits, pMD18-T vector, PyrobestTM DNA polymerase and TaqTM DNA polymerase in combination (TaKaRa Biotechnology Co., Ltd., Dalian, China, Fax: 0411-7619946, Tel: 0411-7611410).

characteristic: lab-host

text: Neopyropia yezoensis

text: RcoR V with a T overhang

text: Filamentous sporophyte

text: Porphyra yezoensis filamentous sporophyte cDNA library

text: Plasmid


text: Children's Memorial Research Center

characteristic: INSDC center name

text: live

text: Generic

text: The UI-R-DK0 library is a subtracted library derived from a mixture of five individually tagged normalized rat libraries: brain-nRBP (20%), heart-nRHP (20%), kidney-nRKP (20%), aorta-nRAP (20%), and placenta-nRPP (20%). Each original library was constructed from a mixture of equal amounts of RNA from seven different developmental time-points: embryonic day 17, embryonic day 19, embryonic day 21, adult day 1, adult day 12, adult day 75, and adult day 200. (Exception: the aorta pool does not contain embryonic day 17 RNA and the placenta pool contains only the three embryonic stages). Each library was normalized individually according to the procedure described by Bonaldo, Lennon & Soares (Genome Research Genome 6: 791-806, 1996). For construction of the DK0 subtracted library, plasmid DNA from each of the five individually tagged normalized libraries was mixed in the proportions specified above and electroporated into competent bacteria for production of single-stranded circular DNA representing the pool of libraries. Single-stranded circular DNA representing these five normalized libraries was then used as a tracer in a subtractive hybridization with a driver (PCR amplified inserts from a plasmid DNA template preparation) comprising: a) a set of about 1,000 arrayed clones from each of the five non-normalized libraries of brain (CT0s), heart (CS0s), kidney (CU0s), aorta (CW0s), and placenta (CX0s). The resulting pool of approximately 5,000 clones represented about 33.3% of the final driver population. A set of about 2,000 arrayed clones from each of the five normalized libraries of brain (CT0), heart (CS0), kidney (CU0), aorta (CW0), and placenta (CX0). The resulting pool of about 10,000 clones represented about 66.6% of the final driver population.

text: ADULT

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid


text: Children's Memorial Research Center

text: live

text: Generic

text: The UI-R-DL0 library is a non-normalized Rat Tongue library constructed in pT3T7 PAC vector according to the procedure described by Bonaldo, Lennon & Soares (Genome Research Genome 6: 791-806, 1996). The oligonucleotide used to prime first strand synthesis contained the sequence tag GCGAA between the Not I cloning site and dT18 stretch.

text: ADULT

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid


text: Children's Memorial Research Center

text: live

text: Generic

text: The UI-R-DM0 library is a non-normalized Rat Prostate library constructed in pT3T7 PAC vector according to the procedure described by Bonaldo, Lennon & Soares (Genome Research Genome 6: 791-806, 1996). The oligonucleotide used to prime first strand synthesis contained the sequence tag CCAGG between the Not I cloning site and dT18 stretch.

text: ADULT

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid


text: Children's Memorial Research Center

text: live

text: Generic

text: The UI-R-DN0 library is a non-normalized Rat Distal Colon library constructed in pT3T7 PAC vector according to the procedure described by Bonaldo, Lennon & Soares (Genome Research Genome 6: 791-806, 1996). The oligonucleotide used to prime first strand synthesis contained the sequence tag CCAGG between the Not I cloning site and dT18 stretch.

text: ADULT

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid


text: Children's Memorial Research Center

text: live

text: Generic

text: The UI-R-DO0 library is a non-normalized Rat Cell Line R3327-5P library constructed in pT3T7 PAC vector according to the procedure described by Bonaldo, Lennon & Soares (Genome Research Genome 6: 791-806, 1996). The oligonucleotide used to prime first strand synthesis contained the sequence tag CCAGG between the Not I cloning site and dT18 stretch.

text: ADULT

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid


text: Children's Memorial Research Center

text: live

text: Generic

text: The UI-R-DQ0 library is a non-normalized Rat Cell Line R3327-5A library constructed in pT3T7 PAC vector according to the procedure described by Bonaldo, Lennon & Soares (Genome Research Genome 6: 791-806, 1996). The oligonucleotide used to prime first strand synthesis contained the sequence tag CCAGG between the Not I cloning site and dT18 stretch.

text: ADULT

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid


text: Children's Memorial Research Center

text: live

text: Generic

text: The UI-R-DR0 library is a non-normalized Rat Osteoblast library constructed in pT3T7 PAC vector according to the procedure described by Bonaldo, Lennon & Soares (Genome Research Genome 6: 791-806, 1996). The oligonucleotide used to prime first strand synthesis contained the sequence tag CCAGG between the Not I cloning site and dT18 stretch.

text: ADULT

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid


text: Children's Memorial Research Center

text: live

text: Generic

text: The UI-R-DS0 library is a non-normalized Rat Appendix library constructed in pT3T7 PAC vector according to the procedure described by Bonaldo, Lennon & Soares (Genome Research Genome 6: 791-806, 1996). The oligonucleotide used to prime first strand synthesis contained the sequence tag CCAGG between the Not I cloning site and dT18 stretch.

text: ADULT

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid


text: Children's Memorial Research Center

text: live

text: Generic

text: CV2 is a subtracted library derived from a normalized eye library (nREE2), constructed according to the procedure described by Bonaldo, Lennon & Soares (Genome Research Genome 6: 791-806, 1996). For construction of the CV2 library, plasmid DNA from the normalized rat eye library was electroporated into competent bacteria for production of single-stranded circular DNA. This was then used as a tracer in a subtractive hybridization with a driver comprising: a) a set of about 1,000 arrayed clones from the non-normalized eye library CV0 (PCR amplified inserts from a plasmid DNA template preparation representing plates R-CV0-BRH through R-CV0-BRR). This represented 20% of the final driver population. b) A set of about 4,000 arrayed clones from the normalized eye library CV1 (PCR amplified inserts from a plasmid DNA template preparation representing plates R-CV1-BRS through R-CV1-BSC, R-CV1-BSE through R-CV1-BTC, and R-CV1-BV0 through R-CV1-BVU). This represented 80% of the final driver population.

text: ADULT

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid


text: Stazione Zoologica 'Anton Dohrn'

text: live

text: Generic

characteristic: cell-line

text: Phaeodactylum tricornutum

text: Eco RI

text: Xho I

text: Phaeodactylum tricornutum Uni-Zap XR

text: phage

text: Uni-Zap XR vector


text: Baylor College of Medicine

text: live

text: Generic

characteristic: cultivar

text: Immature seeds, collected from pods ranging in age from 11 to 19 days after pollination, were harvested from greenhouse-grown plants. Seeds were removed and separated from pod walls and were immediately frozen in liquid nitrogen. Seeds throughout the age range were pooled for mRNA extraction. cDNA was prepared from polyA+ enriched RNA. The cDNA was directionally ligated into the Unizap XR vector from Stratagene and packaged using Gigapack III Gold packaging extracts. Plasmids containing cDNA inserts were excised from the recombinant lambda-Zap phage using Ex-assist helper phage and propogated in XLOLR cells.

text: Immature seeds, 11 to 19 days after pollination

text: Medicago truncatula

text: EcoRI

text: XhoI

text: immature seeds

text: GESD

text: pBluescript SK-


text: Baylor College of Medicine

characteristic: INSDC first public

text: live

text: Generic

text: Immature pods, ranging in age from 15 to 30 days after pollination, were collected from greenhouse-grown plants. At harvest, seeds were removed from pods and isolated pod walls were collected and immediately frozen in liquid nitrogen. Pod walls were pooled for mRNA extraction. cDNA was prepared from polyA+ enriched RNA. The cDNA was directionally ligated into the Unizap XR vector from Stratagene and packaged using Gigapack III Gold packaging extracts. Plasmids containing cDNA inserts were excised from the recombinant lambda-Zap phage using Ex-assist helper phage and propogated in XLOLR cells.

text: Medicago truncatula

text: EcoRI

text: XhoI

text: immature pod walls

text: GPOD

text: pBluescript SK-


text: Washington University School of Medicine

characteristic: INSDC last update

text: live

text: Generic

text: Bragg NTS382

text: The mRNA was isolated from roots of 7 day old 'Bragg' supernodulating mutant NTS382 seedlings that were infected with Bradyhizobium japonicum, strain USDA 110, 48 hours prior to harvest. Dr. Gary Stacey generously donated the tissue. The roots were flash-frozen in liquid nitrogen. Stratagene's cDNA Synthesis Kit (catalog number 200401) was used to synthesize the cDNA. First-strand synthesis was performed with 5-methyl dCTP, hence the ligated cDNA was hemimethylated. A modification of Stratagene's first-strand synthesis primer was used. An 'anchor' nucleotide (V=A, C, or G) was added to the 3' end of the primer [GAGAGAGAGAGAGAGAGAGAACTAGTCTCGAG(T)18V] to anchor the primer at the 5' end of the poly(A) tract. After second-strand synthesis, the cDNA ends were filled in with cloned Pfu DNA, ligated to EcoRI adapters and subsequently phosphorylated. The cDNA was then precipitated and redissolved in sterile, RNase-, DNase-free water. The XhoI site within the first-strand synthesis primer was then restricted by digestion with XhoI from Promega (40U/ul); all XhoI sites in the cDNA would be protected by their hemimethylated status. The cDNA constructs were size-fractionated with a 500bp cutoff, using Sephacryl S-500 High Resolution (Pharmacia Biotech) in a 2-mm diameter column and a bed volume of approximately 1ml. The column eluent was precipitated, redissolved, and ligated into Stratagene's pBluescript II XR Predigested vector (pBluescript II SK(+) vector that has been digested with EcoRI and XhoI, and phosphorylated by Stratagene). This library was constucted in the laboratory of Dr. Paul Keim and Dr. Virginia H. Coryell at Northern Arizona University.

text: 7 days old

text: Glycine max

text: EcoRI

text: XhoI

text: Roots of 7 day old 'Bragg' supernodulating mutant NTS382 seedlings

text: plasmid

text: pBluescript II SK+


text: live

text: Generic

text: Bragg

text: The mRNA was isolated from roots of 8 day old 'Bragg' seedlings that were mock-infected 72 hours prior to harvest. Dr. Gary Stacey generously donated the tissue. The roots were flash-frozen in liquid nitrogen. Stratagene's cDNA Synthesis Kit (catalog number 200401) was used to synthesize the cDNA. First-strand synthesis was performed with 5-methyl dCTP, hence the ligated cDNA was hemimethylated. A modification of Stratagene's first-strand synthesis primer was used. An 'anchor' nucleotide (V=A, C, or G) was added to the 3' end of the primer [GAGAGAGAGAGAGAGAGAGAACTAGTCTCGAG(T)18V] to anchor the primer at the 5' end of the poly(A) tract. After second-strand synthesis, the cDNA ends were filled in with cloned Pfu DNA, ligated to EcoRI adapters and subsequently phosphorylated. The cDNA was then precipitated and redissolved in sterile, RNase-, DNase-free water. The XhoI site within the first- strand synthesis primer was then restricted by digestion with XhoI from Promega (40U/ul); all XhoI sites in the cDNA would be protected by their hemimethylated status. The cDNA constructs were size-fractionated with a 500bp cutoff, using Sephacryl S-500 High Resolution (Pharmacia Biotech) in a 2-mm diameter column and a bed volume of approximately 1ml. The column eluent was precipitated, redissolved, and ligated into Stratagene's pBluescript II XR Predigested vector (pBluescript II SK(+) vector that has been digested with EcoRI and XhoI, and phosphorylated by Stratagene). This library was constucted in the laboratory of Dr. Paul Keim and Dr. Virginia H. Coryell at Northern Arizona University.

text: 8 days old

text: Glycine max

text: EcoRI

text: XhoI

text: Roots of 8 day old 'Bragg' seedlings

characteristic: tissue-type

text: plasmid

text: pBluescript II SK+


text: Harvard University, Howard Hughes Medical Institute

text: live

characteristic: INSDC status

text: Generic

text: Five libraries representing EI0.5/12.5 pancreatic bud, E16.5 pancreas, newborn pancreas, adult pancreas, and adult islets of Langerhans were seperately constructed using SuperScript Plasmid Library kit (Life Technologies). cDNA was made by oligo-dT priming and size-selected by column fractionation. Libraries were amplified once on solid support and plasmid DNA from each library was prepared and mixed in equal amounts. The mixed library DNA was normalized by method #4 from Bonaldo, Lennon, and Soares 1996 Genome Research 6:791-806; 0.5 microgram single-stranded mixed library plasmid DNA was mixed with 5 micrograms PCR product representing mixed library inserts and hybridized to an Ecot of 6. Single-stranded (unhybridized) plasmids were isolated by hydroxyapatite chromatography and used to make this library.

text: Embryonic day 10.5, E12.5, E16.5, newborn, adult, mixed

text: Mus musculus

text: Not I

text: Sal I

text: Both for embryonic & newborn, male for adult and adult islet

characteristic: sex

text: ICR

characteristic: strain

text: Melton Normalized Mixed Mouse Pancreas 1 N1-MMS1

text: plasmid (ampicillin resistant)


text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Williams

text: The mRNA was isolated from cotyledons of 18-day-old 'Williams' seedlings which were greenhouse grown in potting soil. The cotyledons were flash- frozen in liquid nitrogen. Stratagene's cDNA Synthesis Kit (catalog number 200401) was used to synthesize the cDNA. First-strand synthesis was performed with 5-methyl dCTP, hence the ligated cDNA was hemimethylated. A modification of Stratagene's first-strand synthesis primer was used. An 'anchor' nucleotide (V=A, C, or G) was added to the 3' end of the primer [GAGAGAGAGAGAGAGAGAGAACTAGTCTCGAG(T)18V] to anchor the primer at the 5' end of the poly(A) tract. After second-strand synthesis, the cDNA ends were filled in with cloned Pfu DNA, ligated to EcoRI adapters and subsequently phosphorylated. The cDNA was then precipitated and redissolved in sterile, RNase-, DNase-free water. The XhoI site within the first- strand synthesis primer was then restricted by digestion with XhoI from Promega (40U/ul); all XhoI sites in the cDNA would be protected by their hemimethylated status. The cDNA constructs were size-fractionated with a 500bp cutoff, using Sephacryl S-500 High Resolution (Pharmacia Biotech) in a 2-mm diameter column and a bed volume of approximately 1ml. The column eluent was precipitated, redissolved, and ligated into Stratagene's pBluescript II XR Predigested vector (pBluescript II SK(+) vector that has been digested with EcoRI and XhoI, and phosphorylated by Stratagene). This library was constucted in the laboratory of Dr. Paul Keim and Dr. Virginia H. Coryell at Northern Arizona University.

text: 18 day old 'Williams' seedlings

characteristic: dev-stage

text: Glycine max

text: EcoRI

text: XhoI

text: 18 day old 'Williams' seedlings

text: plasmid

text: pBluescript II SK+


text: live

text: Generic

text: The library was constructed by Brandi Chiapelli and Dr. James McCarter at Washington University, St. Louis. The cDNA was made by using Dynabead oligo-dT priming (Dynal). PCR based library using a modified protocol from the SMART PCR cDNA Synthesis Kit from Clontech. Directionally cloned into the UDG sites of pAMP1. Nematodes were provided by Dr. Warwick Grant of AgRearch, New Zealand (warwick.grant@agresearch.co.nz).

characteristic: description

text: Free Living

text: Parastrongyloides trichosuri

characteristic: organism

text: NotI

text: SalI

text: Parastrongyloides trichosuri FL pAMP1 v1 Chiapelli McCarter

text: plasmid (ampicillin resistant)

text: pAMP1 (Gibco)

characteristic: vector


characteristic: External Id

text: live

text: Generic

text: Infective Larvae

text: Parastrongyloides trichosuri

text: NotI

text: SalI

text: Parastrongyloides trichosuri IL pAMP1 v1 Chiapelli McCarter

characteristic: title

text: plasmid (ampicillin resistant)

text: pAMP1 (Gibco)


text: CNRS, INSERM, ULP

characteristic: INSDC center name

text: live

text: Generic

text: Oligo dT cDNA library constructed from RNA pooled from segmentation stage zebrafish embryos

text: segmentation stage embryos

text: Danio rerio

text: EcoRI

text: XhoI

text: segmentation stage cDNA library

text: Lambda Zap


text: live

text: Generic

text: The Cassava EYC library1 cDNA library was constructed using the directional cloning kit of Amersham Pharmacia and is directionally cloned into the EcoRI and NotI sites of the vector pYES2. This library was constructed by M. Fregene and R. Terauchi.

text: DH5alpha (E.coli)

characteristic: lab-host

text: Manihot esculenta

text: NotI

text: EcoRI

text: Cassava EYC library1

text: plasmid


text: German Diabetes Research Institute

text: live

text: Generic

text: macrophage

characteristic: cell type

text: J774 A.1 (German Collection of Microorganism and Cell Culture, Braunschweig, Germany)

characteristic: cell-line

text: Total RNA was isolated by Trizol reagent; poly(A)+RNA was purified using oligotex purification system (Qiagen). After double cDNA synthesis Subtractive Suppression Hybridization was performed using PCR-Select cDNA subtraction kit (Clontech). The subtracted cDNA library was cloned into pCRII.1 vector system (Invitrogen). The differentially expressed messages were evaluated by southern blot as first screening step and then confirmated by northern blot.

text: Mouse recombinant IL-10 stimulated macrophages (16 h)

text: Mus musculus

text: Differentially expressed library from murine macrophage after IL-10 stimuli

text: pCRII.1 vector system (Invitrogen)


text: CNRS, INSERM, ULP

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Random primed cDNA library constructed from RNA pooled from embryos from 18 to 48 hpf.

text: 18 hrs to 48 hrs old embryos

text: Danio rerio

text: EcoRI

text: EcoRI

text: random primed zebrafish embryo

text: Lambda Zap


text: AgResearch

text: live

characteristic: INSDC status

text: Generic

text: Ovis aries

text: Sheep cDNA library


text: University of Minnesota

text: live

text: Generic

text: RNA was isolated from a 24-day turkey embryo using a commercially available kit (Stratagene. cDNA library was constructed using a Lambda ZAP (SK-, Stratagene).

text: Meleagris gallopavo

text: whole embryo

characteristic: tissue-type

text: Turkey Lambda ZAP Library

text: Phage

text: Lambda ZAP Library ZAP


text: Washington University School of Medicine

text: live

text: Generic

text: cDNAs were oligo-dT primed and directionally cloned. Staging according to Nieuwkoop and Faber. Library was constructed by N. Garrett, P. LeMaire, A.M. Zorn, and J.B. Gurdon (Wellcome/CRC Institute).

text: DH10B (phage-resistant)

text: Xenopus laevis

text: NotI

text: EcoRI

text: embryo, stage 10.5

text: Wellcome CRC pcDNAI St10 5

text: plasmid (ampicillin resistant)

text: pcDNAI


text: FBN Dummerstorf

text: live

text: Generic

text: lactation

text: Bos taurus

text: female

characteristic: sex

text: mammary gland

text: Cattle pUC18 mammary gland library

text: Plasmid

text: pUC18 Sma I/BAP


text: Southern Illinois University at Carbondale

text: live

text: Generic

characteristic: NCBI submission model

text: Forrest

characteristic: cultivar

text: Forrest roots were inoculated with Fusarium solani f. sp. glycines and samples were collected after 14 days of inoculation. Total RNA was used for cDNA synthesis using SMART PCR cDNA synthesis Kit (CLONTECH, CA) and cloned in pGEM vector. ESTs were sequenced using M13 universal forward or reverse primer.

text: 14 days after inoculation with F. solani

text: Root

characteristic: organ

text: Glycine max

text: cDNA library from Forrest roots infected by F. solani

text: Plasmid

text: pGEM


text: NIDCR/NIH

text: live

text: Generic

characteristic: NCBI submission package

text: Homo sapiens

text: Oral cavity

text: human HNSCC Library


text: AgResearch

text: live

text: Generic

text: Cervus elaphus

characteristic: organism

text: Deer Antler


text: South China Institute of Botany, Academia of China

text: live

text: Generic

text: Oryza sativa

text: stem

text: Oryza sativa stem


text: Oklahoma State University

text: live

text: Generic

text: Bos taurus

text: adipose

text: bovine adipose cDNA


text: Columbia Univ & SLR Hosp Center

text: live

text: Generic

text: adult

characteristic: dev-stage

text: Homo sapiens

text: Human abdominal aortic adventitia from an aneurysm specimen


characteristic: External Id

text: Max-Planck-Institut fuer Molekulare Genetik

text: live

text: Generic

text: OligodT primed and directionally cloned in pSport1 vector using a NotI (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC (T)15-3' and a SalI 5'- TCGACCCACGCGTCCG-3'adapters (Gibco BRL).

characteristic: description

text: 5-6 hrs (gastrula stage)

text: E.coli, XL1 blue

text: Branchiostoma floridae

text: SalI, KpnI, EcoRI (5')

text: NotI, BamHI, HindIII (3')

text: whole embryo

text: Amphioxus 5-6 hrs cDNA library (Name convention: BFLG or MPMGp498)

text: Plasmid

characteristic: vector


text: Kanazawa Univ Sch Med

characteristic: INSDC center name

text: live

text: Generic

text: Rattus norvegicus

text: Rattus norvegicus library (Saijoh K)

characteristic: title


text: live

text: Generic

text: 26 hrs (neurula stage)

text: E.coli, XL1 blue

characteristic: lab-host

text: Branchiostoma floridae

text: SalI, KpnI, EcoRI (5')

text: NotI, BamHI, HindIII (3')

text: whole embryo

text: Amphioxus 26hr cDNA library (Name convention: BFL26 or MPMGp531)

text: Plasmid

text: pSport1 (Gibco BRL)


text: University of Chicago Medical Center

characteristic: INSDC first public

text: live

text: Generic

text: CD34+ stem/progenitor cells

characteristic: cell type

text: 3'ESTs converted from the SAGE tag sequences using GLGI method

text: Homo sapiens

text: Bone marrow

text: cDNA Library from human CD34+ stem/progenitor cells


text: Shanghai Institute of Endocrinology

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: kidney

text: Rattus norvegicus

text: male

text: Sprague-Dawley

characteristic: strain

text: Diabetic hypertensive kidney related mRNA


text: INRA

text: live

text: Generic

characteristic: NCBI submission model

text: SMART amplified cDNAs of 1 cell stage embryos subtracted to SMART amplified cDNAs of morula stage embryos by SSH

text: Morula stage

text: Oryctolagus cuniculus

text: Rabbit embryo cDNA subtracted library

text: Plasmid


text: University of Delaware

text: live

text: Generic

text: Library made from equivalent pools of total RNA isolated from each tissue at different ages. Single pass sequencing from 5'-end

text: Embryonic (d12,d14,d19); post-hatch (w1,w3,w5,w7,w9)

text: E. Coli EMDH10B

text: Gallus gallus

text: Male and Female

characteristic: sex

text: Commercial broiler chicken

text: Pituitary Gland/Hypothalamus/Pineal Gland

characteristic: tissue-type

text: Primary Chicken Pituitary/Hypothalamus/Pineal Library


text: University of Delaware

text: live

text: Generic

characteristic: NCBI submission package

text: E. Coli EMDH10B

text: Gallus gallus

text: Male and Female

text: Commercial broiler chicken

text: Normalized Chicken Pituitary/Hypothalamus/Pineal Library


text: Tufts University School of Veterinary Medicine

text: live

text: Generic

text: Obtained by 5'RACE following the protocol of Gibco BRL. cDNA was synthesized using a gene-specific (IL1-ra) primer (GSP1: 5'-CTGTTTGAGCGGATGAAGGT-3') from a genomic sequence. dCTP was used to add a homopolymeric tail in the 3'end and the tailed cDNA was amplified by PCR using an anchor IL-1ra gene-specific primer (GSP2: 5'-CAUCAUCAUCAUCTGTTTGAGCGGATGAAGGTGAA-3') as the reverse and a Universal Anchor Primer (Gibco BRL) as the forward primer. cDNA was cloned through UDG cloning method.

text: Foal

text: E. coli DH5

text: Equus caballus

characteristic: organism

text: Blood cells (buffy coat) from a septic female

text: 5'RACE cDNA

text: Plasmid

text: pAMP1 (Gibco BRL)


text: The Institute for Genomic Research

text: live

text: Generic

text: Combination of untreated and NGF-treated PC12 cell libraries, and Bento Soares normalized libraries of ovary, brain, kidney, liver, placenta, lung, embryo, skeletal muscle, spleen, heart

text: Rattus norvegicus

text: mixed tissue

text: Rat gene index, normalized rat, norvegicus


text: Washington University School of Medicine

text: live

text: Generic

text: The library was constructed by Brandi Chiapelli and Dr. James McCarter at Washington University, St. Louis. The cDNA was made by using Dynabead oligo-dT priming (Dynal). PCR based library using a modified protocol from the SMART PCR cDNA Synthesis Kit from Clontech. Directionally cloned into the UDG sites of pAMP1. Nematodes are the OP25 strain. Frozen J2 nematodes were provided by Dr. Rick Davis of North Carolina State University

text: enriched for 2nd stage juveniles

characteristic: dev-stage

text: Heterodera glycines

text: NotI

text: SalI

text: Heterodera glycines J2 pAMP1 v8 Chiapelli McCarter

text: plasmid (ampicillin resistant)

text: pAMP1 (Gibco)


characteristic: External Id

text: University of Washington

text: live

text: Generic

text: Ageratum conyzoides (Kanzaki et al, Biosci. Biotechnol. Biochem., 62 (12)2328-2333, 1998)

characteristic: cell-line

text: cDNA-AFLP technique. Ageratum conyzoides plant cells were collected 24 and 48 h after Agrobacterium tumefaciens- or mock- inoculation. RNA was extracted, mRNA was isolated, and double-stranded cDNA was synthesized. The cDNA was digested with TaqI and AseI, adapters were ligated to the termini, and two rounds of PCR were performed. On the first round the cDNAs were amplified with primers specific for the adapters. The products of this first round were amplified with primers based on the adapters containing two additional bases at the 3' end.

text: 24 and 48 h after either inoculation with Agrobacterium tumefaciens or mock-inoculation

text: Ageratum conyzoides

text: AseI

text: TaqI

text: cell suspension culture


text: University of Kentucky

text: live

text: Generic

text: olfactory organ

characteristic: organ

text: Homarus americanus

text: Male & Female

text: Lobster olfactory organ enriched RDA product

text: lambda zap II

characteristic: vector


text: MIPS

characteristic: INSDC center name

text: live

text: Generic

text: cDNA-collection

characteristic: description

text: adult

text: Homo sapiens

text: SfiIA

text: SfiIB

text: 313 (synonym: hlcc2)

characteristic: title


text: MIPS

characteristic: INSDC first public

text: live

text: Generic

text: cDNA-collection

text: adult

characteristic: lab-host

text: Homo sapiens

text: SfiIA

text: SfiIB

text: 686 (synonym: hlcc3)


text: University of Padua

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: caucasian; Skeletal muscle cDNA was depleted of hundred most expressed mRNAs through an original two steps subtractive procedure

text: Homo sapiens

text: EcoRI

text: NotI

text: female

text: pectoral muscle (after mastectomy)

text: plasmid

text: p0PD (Custom)


text: Zhongshan University

text: live

text: Generic

characteristic: NCBI submission model

text: Oryza sativa

text: Oryza sativa meristem of shoot which grown with six leaves


text: Peking Union Medical College Hospital

text: live

text: Generic

text: female luteal phase

text: Homo sapiens

text: female

characteristic: sex

text: endometrium

text: Human endometrium


text: Iowa State University

text: live

text: Generic

text: The MI-P-CP0 library is derived from uterus. For a detailed description of the library from which this clone was derived, please visit our web site at http://pigest.genome.iastate.edu/. The procedure used to create this library has been previously described (Bonaldo, Lennon and Soares, Genome Research 6:791-806, 1996)

text: DH10B (Life Technologies)

text: Sus scrofa

text: Not I

text: EcoRI


text: Iowa State University

text: live

text: Generic

text: The MI-P-CP1 library is normalized library derived from the MI-P-CP0 library, ultimately derived from uterus tissue. For a detailed description of the library from which this clone was derived, please visit our web site at http://pigest.genome.iastate.edu/. The procedure used to create this library has been previously described (Bonaldo, Lennon and Soares, Genome Research 6: 791-806, 1996)

text: DH10B (Life Technologies)

text: Sus scrofa

text: Not I

text: EcoRI

text: crossbreed

characteristic: strain


text: Iowa State University

text: live

text: Generic

characteristic: NCBI submission package

text: The MI-P-H5 library is derived from hypothalamus at estrus day 0. For a detailed description of the library from which this clone was derived, please visit our web site at http://pigest.genome.iastate.edu/.

text: DH10B (Life Technologies)

text: Sus scrofa

text: Not I

text: EcoRI

text: crossbreed


text: Iowa State University

text: live

text: Generic

text: The MI-P-NA is a normalized library comprised of anterior pituitary tissue at estrus days 0, 5, and 12. For a detailed description of the library from which this clone was derived, please visit our web site at http://pigest.genome.iastate.edu/.

text: DH10B (Life Technologies)

text: Sus scrofa

characteristic: organism

text: Not I

text: EcoRI

text: crossbreed

text: MI-P-NA


text: USDA/ARS/UC-Berkeley

text: live

text: Generic

text: Solanum lycopersicum

text: mature anther

characteristic: tissue-type

text: Lycopersicon esculentum mature anther


characteristic: External Id

text: Hirosaki Industrial Research Institute

text: live

text: Generic

text: Fuji

characteristic: cultivar

text: Shoots maintained in vitro culture for 2 years

text: Malus domestica

text: shoot

text: Apple shoot cDNA library


text: Universite degli Studi di Urbino

text: live

text: Generic

text: The cDNA library was constructed from 1 g of total RNA from 5-month-old Tilia platyphyllos-Tuber borchii ectomycorrhiza using the SMART cDNA Library Construction kit (K1051-1, Clontech, Palo Alto, CA, USA) according to the manufacturer's instructions. The recombinant TriplEx2 phages were converted into the corresponding pTriplEx2 plasmids by cre-lox excision in E. coli BM25.8.

text: symbiotic, 5 months after contact

characteristic: dev-stage

text: Tilia platyphyllos Scop.

text: ectomycorrhiza

characteristic: organ

text: Tuber borchii

text: Sfi IA

text: Sfi IB

text: mantle and Hartig net hyphae

text: cDNA library of 5-month-old Tilia platyphyllos-Tuber borchii ectomycorrhiza

text: phage


text: AgResearch

characteristic: INSDC center name

text: live

text: Generic

text: Cervus elaphus

text: Deer ESTs


text: live

text: Generic

text: Bragg NTS382

text: The mRNA was isolated from roots of 8 day old 'Bragg' supernodulating mutant NTS382 seedlings that were infected with Bradyhizobium japonicum, strain USDA 110, 72 hours prior to harvest. Dr. Gary Stacey generously donated the tissue. The roots were flash-frozen in liquid nitrogen. Stratagene's cDNA Synthesis Kit (catalog number 200401) was used to synthesize the cDNA. First-strand synthesis was performed with 5-methyl dCTP, hence the ligated cDNA was hemimethylated. A modification of Stratagene's first-strand synthesis primer was used. An 'anchor' nucleotide (V=A, C, or G) was added to the 3' end of the primer [GAGAGAGAGAGAGAGAGAGAACTAGTCTCGAG(T)18V] to anchor the primer at the 5' end of the poly(A) tract. After second-strand synthesis, the cDNA ends were filled in with cloned Pfu DNA, ligated to EcoRI adapters and subsequently phosphorylated. The cDNA was then precipitated and redissolved in sterile, RNase-, DNase-free water. The XhoI site within the first- strand synthesis primer was then restricted by digestion with XhoI from Promega (40U/ul); all XhoI sites in the cDNA would be protected by their hemimethylated status. The cDNA constructs were size-fractionated with a 500bp cutoff, using Sephacryl S-500 High Resolution (Pharmacia Biotech) in a 2-mm diameter column and a bed volume of approximately 1ml. The column eluent was precipitated, redissolved, and ligated into Stratagene's pBluescript II XR Predigested vector (pBluescript II SK(+) vector that has been digested with EcoRI and XhoI, and phosphorylated by Stratagene). This library was constucted in the laboratory of Dr. Paul Keim and Dr. Virginia H. Coryell at Northern Arizona University.

characteristic: description

text: 8 days old

text: Glycine max

text: EcoRI

text: XhoI

text: Roots of 8 day old 'Bragg' supernodulating mutant NTS382 seedlings

text: plasmid

text: pBluescript II SK+

characteristic: vector


text: Washington University School of Medicine

characteristic: INSDC first public

text: live

text: Generic

text: Library is cloned directionally between the DraIII(X) and DraIII(Y) sites and has been amplified. Library constructed by S. Lin.

text: adult

text: DH10B (phage-resistant)

text: brain

text: Danio rerio

text: DraIII(Y)

text: DRAIII(X)

text: mixed

text: Zebrafish neuronal

characteristic: title

text: phagemid (ampicillin resistant)

text: pBluescript (modified)


characteristic: INSDC last update

text: live

text: Generic

text: Anchor enzyme: NlaIII~ Normal kidney from female, age 53, cause of death IC brain hemorrhage. PolyA RNA obtained from Ambion Inc. Library constructed in the laboratory of G. Riggins, M.D., Ph.D. (Duke University).

characteristic: lab-host

text: kidney

text: Homo sapiens

text: female

text: plasmid


text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Anchor enzyme: NlaIII~ Stomach, Human primary gastric cancer tissue propagated through nude mice (Xenograft). Tissue Description: 57 yrs male, Gastroesophageal junction adenocarcinoma, T4N0M0, moderately differentiated, obtained by surgery. Library constructed in the laboratory of Steven Powell, M.D., and Wa'el El-Rifai, M.D., Ph.D. (Digestive Health Center, University of Virginia Health System).

text: stomach

text: Homo sapiens

text: male

text: tumor

text: plasmid


text: live

text: Generic

text: Anchor enzyme: NlaIII~ Tissue Description: 57 yrs male, Gastroesophageal junction adenocarcinoma, T4N0M0, moderately differentiated, obtained by surgery. Library constructed in the laboratory of Steven Powell, M.D., and Wa'el El-Rifai, M.D., Ph.D. (Digestive Health Center, University of Virginia Health System).

text: stomach

text: Homo sapiens

text: male

characteristic: sex

text: tumor

text: plasmid


text: live

text: Generic

characteristic: NCBI submission package

text: Anchor enzyme: NlaIII~ Tissue Description: normal adult leukocyte from pooled females. Library constructed in the laboratory of G. Riggins, M.D., Ph.D. (Duke University).

text: Homo sapiens

text: female

text: leukocyte

text: plasmid


text: live

text: Generic

text: Anchor enzyme: NlaIII~ Tissue Description: normal adult lung from 20 year old male, cytomegalovirus positive. Library constructed in the laboratory of G. Riggins, M.D., Ph.D. (Duke University).

text: lung

text: Homo sapiens

characteristic: organism

text: male

text: plasmid


text: Weifang Medical college

text: live

text: Generic

text: neuroepithelium

characteristic: cell type

text: embryonic day 9 post-fertilization

text: Mesocricetus auratus

text: Mesocricetus auratus embryonic day 9 neuroepithelium


characteristic: External Id

text: Texas Tech University Health Science Center

text: live

text: Generic

text: Library prepared by insertion of DpnII digested hamster testis cDNA into pBluescript, BamHI digested vector.

text: adult

text: Mesocricetus auratus

text: male

text: testis

characteristic: tissue-type

text: Hamster Bluescript DpnII library


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: cDNAs were oligo-dT primed and directionally cloned. Staging according to Nieuwkoop and Faber. Library is subtracted and was constructed by N. Garrett and A.M. Zorn, (Wellcome/CRC Institute).

text: DH10B (phage-resistant)

text: Xenopus laevis

text: NotI

text: EcoRI

text: egg, subtracted by stage 19-26 animal cap

text: Wellcome CRC pRN3 St19 26 egg animal cap

text: plasmid (ampicillin resistant)