text: Institute of Marine Research

text: live

text: Generic

text: cDNA library constructed from pooled samples from in vivo stimulated Atlantic halibut (Hippoglossus hippoglossus L) injected with vibriosis vaccine and in vitro activated

text: Hippoglossus hippoglossus

text: leukocytes, liver and head kidney

text: Hippoglossus hippoglossus cDNA library from liver, head kidney and leukocytes from peripheral blood and spleen AHB

text: pBluescript II


status: PUBLIC

characteristic: External Id

text: Institute of Marine Research

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Splenocyte

tag: attribute

characteristic: cell type

text: cDNA library constructed from pooled samples of splenocytes from Atlantic halibut (Hippoglossus hippoglossus L) injected with nodavirus and in vitro activated

characteristic: description

text: Hippoglossus hippoglossus

characteristic: organism

text: Hippoglossus hippoglossus cDNA library BSI

characteristic: title

text: pBluescript II

characteristic: vector


text: Institute of Marine Research

text: live

text: Generic

text: Splenocyte

text: cDNA library constructed from pooled samples of atlantic halibut mock injected with L-15 medium

text: Hippoglossus hippoglossus

text: Hippoglossus hippoglossus cDNA library BSU

text: pBluescript II


text: Institute of Marine Research

text: live

text: Generic

text: cDNA library constructed from pooled thymus samples of Atlantic halibut (Hippoglossus hippoglossus L)

text: Hippoglossus hippoglossus

text: Thymus

characteristic: tissue-type

text: Hippoglossus hippoglossus cDNA library from unstimulated thymus cells TEO

text: pBluescript II


text: University of British Columbia

text: live

text: Generic

characteristic: cultivar

text: Buds were harvested from ~10 5 yr old FB3-425 Sitka spruce trees, at the University of British Columbia, 6-8 weeks after bud set. Brown bud scales were removed to extract the developing bud. RNA was isolated from a total of 172 buds. 0.15 micrograms of poly A+ RNA was used by Evrogen (Moscow, Russia) according to methodology described in Zhulidov et al. (2004) Nucleic Acids Res. 32. Clontech's Creator Smart Kit (Mountain View, USA) and Evrogen's Trimmer Direct kit's primers were used to generate a non-normalized cDNA library. Plasmid DNA was then transformed by electroporation into Invitrogen's DH10B-T1 phage resistant cells (Carlsbad, USA). Clones used in sequencing were from the primary library.

text: E. coli DH10B

characteristic: lab-host

text: Picea sitchensis

text: Sfi I

text: Sfi I

text: hermaphrodite

characteristic: sex

text: Bud

text: Plasmid


text: University of British Columbia

text: live

text: Generic

text: Buds were harvested from ~10 5 yr old FB3-425 Sitka spruce trees, at the University of British Columbia, 6-8 weeks after bud set. Brown bud scales were removed to extract the developing bud. RNA was isolated from a total of 172 buds. 0.15 micrograms of poly A+ RNA was used by Evrogen (Moscow, Russia), according to methodology described in Zhulidov et al. (2004) Nucleic Acids Res. 32. Clontech's Creator Smart Kit (Mountain View, USA) and Evrogen's Trimmer Direct kit were used to generate a normalized cDNA library. Plasmid DNA was then transformed by electroporation into Invitrogen's DH10B-T1 phage resistant cells (Carlsbad, USA). Clones used in sequencing were from the primary library.

text: E. coli DH10B

text: Picea sitchensis

text: Sfi I

text: Sfi I

text: hermaphrodite

text: Bud


text: University of British Columbia

text: live

text: Generic

text: Buds were harvested from ~10 5 yr old FB3-425 Sitka spruce trees, at the University of British Columbia, 6-8 weeks after bud set. Brown bud scales were removed to extract the developing bud. RNA was isolated from a total of 172 buds. 1.0 micrograms of poly A+ RNA was used to produce a non-normalized library using Clontech's Creator Smart Kit (Mountain View, USA) and Evrogen's Trimmer Direct kit's primers (Moscow, Russia) to generate a non-normalized cDNA library. cDNA was ligated into pAL17.3 vector, then transformed by electroporation into Invitrogen's DH10B-T1 phage resistant cells (Carlsbad, USA). Clones used in sequencing were from the primary library.

text: E. coli DH10B

text: Picea sitchensis

text: Sfi I

text: Sfi I

text: hermaphrodite

text: Bud

text: Plasmid


text: University of British Columbia

text: live

text: Generic

text: Buds were harvested from ~10 5 yr old FB3-425 Sitka spruce trees, at the University of British Columbia, 6-8 weeks after bud set. Brown bud scales were removed to extract the developing bud. RNA was isolated from a total of 172 buds. 1.0 micrograms of poly A+ RNA was used to produce a normalized cDNA library used according to methodology described in Zhulidov et al. (2004) Nucleic Acids Res. 32. We used Clontech's Creator Smart Kit (Mountain View, USA) and Evrogen's Trimmer Direct kit (Moscow, Russia) with some modifications. cDNA was ligated into pAL17.3 vector, then transformed by electroporation into Invitrogen's DH10B-T1 phage resistant cells (Carlsbad, USA). Clones used in sequencing were from the primary library.

text: E. coli DH10B

text: Picea sitchensis

text: Sfi I

text: Sfi I

text: hermaphrodite

text: Bud

text: Plasmid


text: Yunnan Agricultural University

text: live

text: Generic

text: breed: black-boned

text: Ovis aries

text: longissimus dorsi muscle

text: black-boned sheep muscle full-length cDNA library

text: pDNR-LIB Vector


text: Nanjing Agricultural University

text: live

text: Generic

text: cDNA fragments of M1 progeny which produced by the nitrogen ions dose of 0.39 1016 N+/cm2 implanting into pollen of upland, which was pollinated to the pistil.

text: Gossypium hirsutum

text: leaf blade

text: Gossypium hirsutum leaf blade of M1 progeny


text: Universidad de Malaga, Facultad de ciencias

text: live

text: Generic

text: ecotype: Corsican; A composite cDNA library was made with mRNA isolated from normal, compression, opposite, early and late wood of Maritime pine uni-directionally cloned into Uni-ZAP XR using the ZAP-cDNA Synthesis kit (Stratagene). pBluescript SK(-) plasmids were obtained by in vivo massexcision. The nucleotide sequence of the 5'end was obtained by automated sequencing with the T3 primer by GENOME EXPRESS, Meylan, France.

text: Pinus pinaster

text: EcoRI

text: Xho I

text: differentiating xylem

text: Pinus pinaster Corsican differentiating xylem

text: Uni-Zap XR lambda (Stratagene)


text: Newcastle University

text: live

text: Generic

text: adult

characteristic: dev-stage

text: Amphibalanus amphitrite

text: whole soft tissues


characteristic: External Id

text: National Research Council

text: live

text: Generic

text: Violetto di Provenza

text: Cynara cardunculus var. scolymus

text: leaves

text: Cynara scolymus leaves


text: University of Oslo

characteristic: INSDC center name

text: live

text: Generic

text: Breviata anathema

characteristic: strain

text: Breviata anathema ATCC:50338 EST


text: ETH Zurich

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: A normalized cDNA library was constructed from polyA+ RNA isolated from flower buds and open flowers of male and female S. latifolia grown in a greenhouse at ETH Zurich. Three tissue pools were prepared. Pool A included petals from fully open male and female flowers; pool B consisted of anthers and male flower buds; pool C contained stigmas, ovaries, and female flower buds. Three adaptors(with specific tags, TCG, GAG or ATG) were incorporated into the 5' and 3' ends to distinguish between cDNAs isolated from the different tissue types: cDNA A: 5'end (EcoRI site): 5'-GAATTCgtgagccagaggacgagacaaTCGaa-3' ; 3'end (NotI site): 5'-GCGGCCGCTCG-3' ; cDNA B: 5'end (EcoRI site): 5'-GAATTCgtgagccagaggacgagacaaGAGaa-3' ; 3'end (NotI site): 5'-GCGGCCGCGAG-3' ; cDNA C: 5'end (EcoRI site): 5'-GAATTCgtgagccagaggacgagacaaATGaa-3' ; 3'end (NotI site): 5'-GCGGCCGCATG-3'. Sequences were obtained on a ABI PRISM 3130xl Genetic Analyzer (Applied Biosystems) and extracted from the chromatograms using the PHRED software. Vector, adaptors (library tags) and potential E.coli contaminant sequences were removed using SeqClean. Poly-A sequences were detected and trimmed using SeqClean.

text: flowers

characteristic: organ

text: Silene latifolia

text: Eco RI

text: NotI

text: male or female

text: mixed

text: Silene latifolia male and female flowers, Normalized

text: pBSII SK(+)


text: Chinese Academy of Forestry

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Total RNAs were extracted from cambial zone and immature leaf. The cambium zone (tester) versus immature leaf (driver) subtractive library was constructed using SSH. The subtracted PCR products generated by SSH were inserted into the pGEM-T vector and transformed into DH5alpha. Positive clones with an insert longer than 100 bp were isolated, and spotted onto poly-L-lysine-coated slides. By microarray analysis, more than 200 genes were found to be differently expressed at the different stages of the secondary vascular system regeneration. And then, the corresponding clones were sequenced.

text: Populus tomentosa

text: Cambium zone

text: Populus tomentosa cambium zone versus immature leaf subtracted cDNA library

text: Plasmid

text: pGEM T


text: Department of Biology, East China Normal University, Shanghai

text: live

text: Generic

text: adult

text: Eriocheir sinensis

characteristic: organism

text: accessory sex gland

text: Eriocheir sinensis accessory sex gland subtractive cDNA libraries

text: plasmid

text: pGEM -T Easy


text: Insitute of Vegetable Science, Zhejiang University of China

text: live

text: Generic

text: anther development

text: anthers

text: Brassica rapa subsp. chinensis

text: pollen and tapetum

text: cDNA from anthers cultivar Bcajh97-01A/B

characteristic: title


text: International Centre for Genetic Engineering and Biotechnology

text: live

text: Generic

text: Total RNA was isolated from leaf tissue of one month old Greenhouse-grown Jatropha curcas plant. Following preparation of leaf mRNA, subsequent steps of cDNA synthesis and library construction were performed using the ZAP-cDNA Synthesis Kit and ZAP- cDNA Gigapack III Gold Cloning Kit (Stratagene). Seed material provided by Dr. M. P. Reddy [Discipline of Wasteland Research, Central Salt and Marine Chemicals Research Institute, G B Marg, Bhavnagar,Gujarat 364002, India, Discipline of Wasteland Research, Central Salt and Marine Chemicals Research Institute, G B Marg, Bhavnagar, Gujarat, India].

characteristic: description

text: one month old plant

text: Jatropha curcas

text: EcoRI

text: XhoI

text: Leaf tissue from healthy plant

text: Jatropha curcas total leaf library

text: Lambda Uni-ZAP XR

characteristic: vector


text: National Institute of Animal Science (Korea)

text: live

text: Generic

text: 24 months old

text: intermuscular fat

text: Bos taurus

text: four males mixed

text: intermuscular fat

characteristic: tissue-type

text: HW cDNA library intermuscular fat

text: Uni-ZAPXR


text: live

text: Generic

text: 24 months old

characteristic: lab-host

text: liver

text: Bos taurus

text: four males mixed

text: liver

text: HW cDNA library liver

text: Uni-ZAPXR


text: live

text: Generic

text: 24 months old

text: loin muscle

text: Bos taurus

text: four males mixed

characteristic: sex

text: longissimus dorsi

text: HW cDNA library longissimus dorsi

text: Uni-ZAPXR


text: JST ERATO, Hashimoto Light Energy Conversion Project

text: live

text: Generic

text: isolation_source: crude oil-degrading marine microbial community. environmental_sample.

text: uncultured bacterium

text: transcripts from crude oil-degrading marine microbial community


text: Liaoning Ocean and Fisheries Science Research Institute

text: live

text: Generic

text: E. coli

text: respiratory tree

text: Apostichopus japonicus

text: Sfi IA

text: Sfi IB

text: respiratory tree

text: sea cucumber SMART cDNA library from respiratory tree

text: pDNR-LIB


text: Huazhong University of Science and Technology

text: live

text: Generic

text: E.coli DH10B

text: Dunaliella salina

text: EcoRI

text: NotI

text: Salt-tolerant Dunaliella Salina cDNA library


text: Shenyang Agricultural University

text: live

text: Generic

text: A full-length cDNA library was conducted by cloning cDNA fragments into pDNR-LIB vector and sequenced at 5' end.

text: Post-diapause

characteristic: dev-stage

text: Antheraea pernyi

text: Female

text: Shenhuang No. 1

text: Pupa

text: Antheraea pernyi pupal cDNA library

text: pDNR-LIB


characteristic: External Id

text: College of Wildlife Resource Northeast Forestry University

text: live

text: Generic

text: velvet tip

text: Cervus nippon

text: male

text: velvet tip

text: Velvet tips tissue cDNA Library of Northeast Sika Deer


text: University of Arizona

characteristic: INSDC center name

text: live

text: Generic

text: Coccidioides posadasii

text: Eco RV

text: Not I

text: Silveira

text: C. posadasii Silveira, MYCELIAL_NORMALIZED

text: Plasmid


text: University of Arizona

text: live

text: Generic

text: Coccidioides posadasii

text: Eco RV

text: Not I

text: Silveira

characteristic: strain

text: C. posadasii Silveira, 24HR SPHERULE_NORMALIZED

text: Plasmid


text: University of Arizona

characteristic: INSDC first public

text: live

text: Generic

text: Coccidioides posadasii

text: Eco RV

text: Not I

text: Silveira

text: C. posadasii Silveira, 72HR SPHERULE_NORMALIZED

text: Plasmid


text: University of Arizona

characteristic: INSDC last update

text: live

text: Generic

text: Coccidioides posadasii

text: Eco RV

text: Not I

text: Silveira

text: C. posadasii Silveira, 120HR SPHERULE_NORMALIZED

text: Plasmid


text: University of Arizona

text: live

text: Generic

text: Coccidioides immitis

text: Eco RV

text: Not I

text: RS

text: C. immitis RS, 48HR MYCELIUM_NORMALIZED

text: Plasmid


text: University of Arizona

text: live

text: Generic

text: Coccidioides immitis

text: Eco RV

text: Not I

text: RS

text: C. immitis RS, 72HR Spherule_NORMALIZED

text: Plasmid


text: University of Arizona

text: live

text: Generic

text: Coccidioides immitis

text: Eco RV

text: Not I

text: RS

text: C. immitis RS, 120HR Spherul_NORMALIZED

text: Plasmid


text: Instituto Potosino de Investigacion Cientifica y Tecnologica

text: live

text: Generic

text: Pinto Villa

characteristic: cultivar

text: Phaseolus vulgaris

text: EcoRI

text: EcoRI

text: Phaseolus vulgaris salinity Subtractive Library

text: Plasmid

text: pCR4-TOPO (Invitrogen)


text: The Miami Project to Cure Paralysis, University of Miami Miller School of Medicine

text: live

characteristic: INSDC status

text: Generic

text: Poly(A)+ mRNA was purified from P7-10 wild type C57BL/6 mouse cerebellum and DRG and used to carry out cDNA library construction using Invitrogen's SuperScript Choice System for cDNA Synthesis. Blunt, double-stranded cDNAs were size-selected on an agarose gel and prepared for directional cloning in the Stratagene Lambda Zap-CMV XR vector (Agilent Technologies, Santa Clara CA) by sequential ligation to a 500-fold molar excess of EcoR1 adaptor and Xho1 digestion. Lambda ZAP-CMV cDNA ligations were packaged into lambda particles using Gigapack III Gold Packaging extracts (Stratagene). Mass library-wide excision of the phagemid resident in the lambda library was carried out using reagents and protocols provided by the Lambda Zap-CMV XR vendor (Stratagene). The mass-excision process yielded a pCMV-Script EX mammalian-expression phagemid library. The excised mammalian expression libraries in pCMV-Script EX were used to carry out subtractions (DRG - cerebellum) essentially as previously described (Bonaldo et al., 1996). Subtracted hybridizations were processed by Hydroxyapatite (HAP) chromatography to remove double-stranded cDNAs resulting from hybridization of DRG single-stranded circles to complementary cerebellum PCR products. DRG single-stranded circles with no complement in the cerebellar PCR product population remain single-stranded, are not retained by the HAP column and represent mRNA species expressed either exclusively or predominantly in DRG. Recovered single-stranded circles that do not adhere to the HAP column were desalted, concentrated and used in a primer extension reaction (T3 primer) to generate partially double-stranded plasmids fit for electroporation. The partially double-stranded plasmid population was electroporated into DH10B bacterial cells to generate the subtracted library.

text: Postnatal day 7-10

text: Mus musculus

text: EcoRI

text: XhoI

text: Doral root ganglion (DRG)

text: Mouse P7-10 subtracted cDNA library containing dorsal root ganglion (DRG) enriched transcripts

text: Plasmid

text: pCMV-Script EX


text: Northwest A&F University

text: live

text: Generic

characteristic: NCBI submission model

text: Xingzi 9104

text: The flag leaf of boot stage wheat

text: Triticum aestivum

text: leaf

text: SSH cDNA library for wheat adult plant resistance to stripe rust

text: pGEM-Teasy


text: University of Manitoba

text: live

text: Generic

characteristic: NCBI submission package

text: Phytophthora infestans

text: Late Blight-Challenged Solanum tuberosum - IDASH products (Phytophthora infestans)


text: University of Manitoba

text: live

text: Generic

text: Solanum tuberosum

characteristic: organism

text: Late Blight-Challenged Solanum tuberosum - IDASH products


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT)-primed cDNA and cloned into vector pDNR-LIB (Clontech Laboratories, Inc., Mountain View, CA) using the SMART system (BD Biosciences, San Jose, CA). To generate the first strand, polyA RNA was primed with an oligo(dT) primer (5'-ATTCTAGAGGCCGAGGCGGCCGACATG(T)30 VN-3', where V = A, G, or C). The second strand was synthesized by PCR using primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' followed by digestion of the SfiI sites. cDNA was size selected using 1.1% agarose gel electrophoresis, then ligated into pDNR-LIB. Library is normalized. Library was created and sequencing performed by the DOE Joint Genome Institute (Walnut Creek, CA).

text: adult

text: DH10B T1-resistant

text: Peromyscus polionotus subgriseus

text: SfiIA

text: SfiIB

text: pooled male and female

text: PO

text: pooled brain and testis

text: CCUA Peromyscus polionotus subgrisieus PO:Br,Ts

characteristic: title

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

characteristic: description

text: adult

text: DH10B T1-resistant

text: Peromyscus maniculatus bairdii

text: SfiIA

text: SfiIB

text: pooled male and female

text: BW

text: pooled brain and testis

text: CCUB Peromyscus maniculatus bairdii BW:Br,Ts

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: adult

text: DH10B T1-resistant

text: Peromyscus maniculatus bairdii

text: SfiIA

text: SfiIB

text: pooled male and female

text: BW

text: pooled liver, kidney, skin

text: CCUC Peromyscus maniculatus bairdii BW:L,K,Sk

text: pDNR-LIB

characteristic: vector


text: DOE Joint Genome Institute

text: live

text: Generic

text: newborn

text: DH10B T1-resistant

text: Peromyscus maniculatus bairdii

text: SfiIA

text: SfiIB

text: pooled male and female

text: BW

text: 3 pooled whole animals

characteristic: tissue-type

text: CCUF Peromyscus maniculatus bairdii BW:Nb

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: 12 days gestation

text: DH10B T1-resistant

characteristic: lab-host

text: Peromyscus maniculatus bairdii

text: SfiIA

text: SfiIB

text: pooled male and female

text: BW

text: pooled whole fetuses

text: CCUG Peromyscus maniculatus bairdii BW:fetus

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: adult

text: DH10B T1-resistant

text: Peromyscus maniculatus bairdii

text: SfiIA

text: SfiIB

text: pooled male and female

characteristic: sex

text: BW

text: pooled spleen

text: CCUH Peromyscus maniculatus bairdii BW:Spl

text: pDNR-LIB


text: Wuhan University

text: live

text: Generic

text: Adult

characteristic: dev-stage

text: Scorpiops jendeki

text: Venom gland

text: Scorpiops jendeki venom gland Library


characteristic: External Id

text: Hokkaido University

text: live

text: Generic

text: country: Japan: Hokkaido ; isolation_source: bovine erythrocyte infected with Theileria orientalis

text: piroplasm stage

text: Theileria orientalis

text: Ikeda

text: Theileria orientalis lambda TriplEx2 cDNA library

text: lambda TriplEx2


text: IEW

characteristic: INSDC center name

text: live

text: Generic

text: cadmium exposed animals

text: adult

text: whole body

characteristic: organ

text: Orchesella cincta

text: mixed

text: lab culture

text: Orchesella cincta SSH Cadmium

text: pGEM-t


text: IEW

characteristic: INSDC first public

text: live

text: Generic

text: gut

text: Orchesella cincta

text: Gut Library Orchesella cincta


text: Nihon University

characteristic: INSDC last update

text: live

text: Generic

text: country: Japan: Hokkaido ; collection_date: 10-Jun-2004

text: tadpole

text: Rana pirica

text: skin of head trunk

text: Rana pirica skin of head trunk tadpole


text: Tokyo University of Marine Science and Technology

text: live

text: Generic

text: hemocyte

characteristic: cell type

text: Panulirus japonicus

text: Panulirus japonicus hemocyte


text: National Institute of Agrobiological Sciences

text: live

characteristic: INSDC status

text: Generic

text: newly-eclosed adult

text: Bombyx mori

characteristic: strain

text: pheromone gland

text: phe-


text: Liaoning Ocean and Fisheries Science Research Institute

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: E. coli

text: body wall

text: Apostichopus japonicus

text: Sfi IA

text: Sfi IB

text: body wall

text: sea cucumber SMART cDNA library from body wall

text: pDNR-LIB


text: live

text: Generic

text: E. coli

text: intestine

text: Apostichopus japonicus

characteristic: organism

text: Sfi IA

text: Sfi IB

text: intestine

text: sea cucumber SMART cDNA library from intestine

characteristic: title

text: pDNR-LIB


text: Tokyo Metropolitan University

text: live

text: Generic

text: grown in a green house

characteristic: description

text: sporophyte

text: Adiantum capillus-veneris

text: young leaves


text: Tokyo Metropolitan University

text: live

text: Generic

text: light-grown

text: gametophyte

text: Adiantum capillus-veneris

text: prothallia

characteristic: tissue-type


text: Tokyo Metropolitan University

text: live

text: Generic

text: light-grown

text: gametophytes with sporophytes

text: Adiantum capillus-veneris

text: prothallia with plantlets


text: Norwegian College of Fishery Science, University of Tromso

text: live

text: Generic

text: Gadus morhua head kidney and spleen normalised cDNA library after stimulation by poly I:C

text: head kidney and spleen

text: Gadus morhua

characteristic: vector


text: Rudjer Boskovic Institute

text: live

text: Generic

text: Lubomirskia baikalensis

text: L. baicalensis Library


text: Rudjer Boskovic Institute

text: live

text: Generic

text: Suberites domuncula

text: S. domuncula Library


text: Western Kentucky University

text: live

text: Generic

text: Cucumis sativus

text: Shoots and roots

text: Cucumis sativus suppression subtractive cDNA library


text: Cassava Molecular Biotechnology Research Consortium, Department of Biotechnology,Faculty of Science, Mahidol University

text: live

text: Generic

characteristic: cultivar

text: mRNA

text: E. coli DH5a

characteristic: lab-host

text: Storage root

text: Manihot esculenta

text: MeKU50 cDNA-AFLP

text: Plasmid


characteristic: External Id

text: Institute of Biotechnology

text: live

text: Generic

text: Envy

text: mix of 60h and 72h old tracheary element differentiating cell culture

characteristic: dev-stage

text: Zinnia elegans

text: xylem cell culture


text: Institute of Biotechnology

characteristic: INSDC center name

text: live

text: Generic

text: Envy

text: Zinnia elegans

text: xylem cell culture

text: SSHCONT


text: MPI for Molecular Genetics

characteristic: INSDC first public

text: live

text: Generic

text: Total RNA was extracted from 10 goldfish specimens of both sex. Fish olfactory epithelia were unilaterally exposed to copper sulfate for 10 min, and tissue from both sides was collected 3 days later. Collected tissue frozen in liq N2 and stored at -80C prior to RNA extraction. Subtractive library construction was performed with PCR-Select cDNA Subtraction Kit (Clontech, Cat. No. 637401). Exposed olfactory epithelium tissue was used as a driver. Sequencing was performed at the MPI of Molecular Genetics Berlin.

text: adult

text: Carassius auratus

text: mixed

characteristic: sex

text: olfactory epithelium

text: GFSR


text: MPI for Molecular Genetics

characteristic: INSDC last update

text: live

text: Generic

text: Total RNA was extracted from 10 goldfish specimens of both sex. Fish olfactory epithelia were unilaterally exposed to copper sulfate for 10 min, and tissue from both sides was collected 3 days later. Collected tissue frozen in liq N2 and stored at -80C prior to RNA extraction. Subtractive library construction was performed with PCR-Select cDNA Subtraction Kit (Clontech, Cat. No. 637401). Unexposed olfactory epithelium tissue was used as a driver. Sequencing was performed at the MPI of Molecular Genetics Berlin.

text: adult

text: Carassius auratus

text: mixed

text: olfactory epithelium

text: GFSF


text: University of South Florida

text: live

characteristic: INSDC status

text: Generic

text: cDNA library created using mRNA enriched RNA isolated from the water column during a Peridinium quinquecorne bloom at 1100 on 21-Aug-2007 (lat: 27.7616, long: -82.6326)

text: marine metagenome

text: Tampa Bay eukaryotic plankton metatranscriptome


text: Vassar College

text: live

text: Generic

text: mRNA isolated from 50 individuals of clone CC7. Large insert library constructed in the pExpress vector by Open Biosystems.

text: adult polyp

text: Exaiptasia diaphana

text: clone CC7

characteristic: strain

text: Aiptasia pallida clone CC7 holobiont cDNA

text: plasmid


text: University of Georgia

text: live

text: Generic

characteristic: NCBI submission model

text: sporozoite

text: Cryptosporidium parvum

text: Eco RV

text: Not I

text: C. parvum KSU-1 normalized sporozoite

text: Plasmid


text: College of Forestry, Beijing Forestry University

text: live

text: Generic

characteristic: NCBI submission package

text: Obtained by cDNA-AFLP.

text: two-year-old seedlings

text: Populus euphratica

text: leaves

text: Populus euphratica early salt-stressed Library


text: Tea Research Association

text: live

text: Generic

text: As in instruction manual of Invitrogen (Catalogue No. K2700) for vector; Blunt ended cDNAs were prepared by using Pfu polymerase of New England Biolabs.

text: Juvenile

text: Leaf

characteristic: organ

text: Camellia sinensis var. sinensis

characteristic: organism

text: Darjeeling Tea B157 Second Flush Sungma

text: Plasmid

text: pBLUNT


text: The University of Queensland

text: live

text: Generic

text: Hemocyte

characteristic: cell type

text: Saccostrea glomerata

text: Saccostrea glomerata SSH1

characteristic: title

text: pCR4 TOPO


text: Sun Yet-sen (Zhongshan) University

text: live

text: Generic

text: Singapore grouper iridorirus (SGIV) infected grouper revealed by suppression subtractive hybridization-forward Library

characteristic: description

text: SGIV 4 days post-infection

text: Epinephelus coioides

text: hermaphrodite

text: spleen

characteristic: tissue-type

text: SGIV infected grouper SSH-forward Library


text: Sun Yet-sen (Zhongshan) University

text: live

text: Generic

text: Singapore grouper iridorirus (SGIV) infected grouper revealed by suppression subtractive hybridization-reverse Library

text: SGIV 4 days post-infection

text: Epinephelus coioides

text: hermaphrodite

text: spleen

text: SGIV infected grouper SSH-reverse Library


text: ANGRAU

text: live

text: Generic

text: Resistance related protein, RNA isolated and cDNA synthesized and amplified using finger millet RGCs specific primers. New England Biolabs (cat.#E6500S).

text: Eleusine coracana

text: leaves

text: Leaves of finger millet


text: Tea Research Association

text: live

text: Generic

text: Mature

characteristic: cultivar

text: Leaf

text: Camellia sinensis var. assamica

text: Assam Tea 3rd and 4th Leaf

text: Plasmid

text: pGEM-T

characteristic: vector


characteristic: External Id

text: Universite de Montreal FMV Sciences Cliniques

text: live

text: Generic

text: Using suppression subtractive hybridization techniques (SSH), lung cDNAs of control horses and asymptomatic principal horses were subtracted from cDNAs of horses with heaves during clinical exacerbation of the disease.

text: Horses with heaves

characteristic: dev-stage

text: Lung

text: Equus caballus

text: Male, female

text: Lung cDNA from heaves-affected horses


text: Roslin Institute

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: Osteoclasts and osteoblasts with some marrow cells

text: suppressive subtraction hybridisation library against liver

text: Adult

text: Bone

text: Gallus gallus

text: female

text: Chicken Bone Library3


text: Roslin Institute

characteristic: INSDC last update

text: live

text: Generic

text: Adult

text: Shell gland or uterus

text: Gallus gallus

text: female

characteristic: sex

text: Chicken Shell Gland Library


text: live

characteristic: INSDC status

text: Generic

text: The red-magenta fully expanded leaves of E. sagittatum growing in Wuhan Botanical Garden, The Chinese Academy of Sciences, were harvested and prepared for RNA isolation. Transcriptome was sequenced by 454 GS-FLX pyrosequencing technology at the Oklahoma University genome center.

text: full-bloom stage

text: Epimedium sagittatum

text: leaf

text: Epimedium sagittatum full-bloom stage leaf


text: College of Life Science, Xinyang Normal University

text: live

text: Generic

characteristic: NCBI submission model

text: young leaves

text: Camellia sinensis

text: Differentially expressed cDNA libraries of tea plant responding to small green leafhopper feeding


text: Federal University of Ceara, Science Center

text: live

text: Generic

characteristic: NCBI submission package

text: Pitiuba

text: Library was prepared from total RNA based on the Gateway Technology (CloneMiner cDNA Library Construction Kit. Invitrogen 18249-029).

text: 10 days after anthesis

text: Seed

text: Vigna unguiculata

text: Developing seed

text: Developing seeds (10 days after anthesis) Library

text: Plasmid

text: pDONR 222


text: Cornell University

text: live

text: Generic

text: Rio Grande-PtoR

text: 3-4 weeks old seedlings were vacuum-infiltrated with a suspension of Pseudomonas syringae pv. tomato DC3000 hrcC minus strain (2x10E8cfu/ml) or left untreated. Leaf samples were collected 2h after treatment and mixed in a 20% untreated, 80% PAMP-elicited weight ratio before RNA extraction. cDNA first strand synthesis used an oligo dT (NotI) primer. cDNAs were directionally cloned into pYESTrp2 (Invitrogen).

text: Solanum lycopersicum

text: EcoRI

text: NotI

text: leaf

text: PAMP-elicited tomato leaf

text: Plasmid


text: Tea Research Association

text: live

text: Generic

text: Mature

text: According to the instructions given in Clontech Manual on PCR Select cDNA Subtraction Kit (Cat. No. 637401; Clontech Lab Inc, USA)

text: Leaf

characteristic: organ

text: Camellia sinensis var. assamica

text: Assam Tea Drought 3rd and 4th Leaf

text: Plasmid

text: pGEM


text: Tea Research Association

text: live

text: Generic

text: Mature

text: According to the instructions given in Clontech Manual on SMART cDNA Library Construction Kit Manual (No. 634901; Clontech Lab Inc, USA)

text: Root

text: Camellia sinensis var. assamica

characteristic: organism

text: Plasmid

text: pTriplX


text: Kasetsart University

text: live

text: Generic

text: The cDNA library was generated as part of Macrobrachium rosenbergii EST project. Total RNA was extracted from androgenic gland of Macrobrachium rosenbergii, 100 prawns used as control group and the other 100 prawns were cut their eyestalk. After 2 weeks, all prawns of each group were dissected to collect the androgenic gland. The mRNA was further purified, reverse transcribed and cloned into Uni-ZAP XR vector.

text: Macrobrachium rosenbergii

text: EcoR I

text: Xho I

text: Androgenic gland

text: Normal eyestalk library and Ablation eyestalk library

text: Phage

text: Uni-ZAP XR


text: Central botanical gardens NAS of Belarus

text: live

text: Generic

text: Loshitsky

text: Experimental model of nearly isogenic (intracultivar) clones of Solanum tuberosum cultivars of Belarusian selection was created in the Institute of Genetic and Cytology of NAS of Belarus on the basis of multistage selection by the trait of resistance to Potato virus X (X) and Potato leaf roll virus (L) (Danilenko, 1995). Obtained 4 types of potato clones of Loshitsky cultivar differed by the resistance to both viruses (resistant (-) to both viruses clones: X-L-; susceptible (+) to both viruses: X+L+; and resistant to one and susceptible to other virus clones: X+L-, X-L+) were used to reveal genome regions, involved in defense reactions against viruses. Loshitsky cultivar is carrying and expressing Rx1 gene (Vlasava N.B., unpublished data). Plants were grown under natural condition. Resistance/susceptibility of clones to viruses was measured by ELISA-test. RNA was isolated from 4 types of clones. cDNAs were generated using an Oligo-dT-adaptor primer. cDNAs of nearly isogenic clones of Solanum tuberosum of cultivar Loshitsky was screened with random primers. Revealed polymorphic cDNA fragments of resistant/susceptible to PVX and/or PLRV clones were excised, purified, cloned into pUC19+ vector and sequenced.

text: Bud stage plants

text: XL1-Blue Electroporation-Competent Cells (Stratagene)

characteristic: lab-host

text: Solanum tuberosum

text: XbaI

text: leaf

text: Polymorphic cDNAs of nearly isogenic clones of cultivar Loshitsky, differed by resistance to PVX and PLRV

text: Plasmid


text: Institut de Recherche pour le Developpement

text: live

text: Generic

text: Tenera Lab 221

text: Suppression subtractive hybridization library; cells cultured for 30 days in liquid proliferation medium containing 2,4-D (proliferation conditions) subtracted against cells cultured for 16 days in liquid pretreatment medium in the absence of 2,4-D (initiation conditions).

text: proliferation in presence of 2,4-D

text: Electro ten-blue Stratagene

text: Elaeis guineensis

text: embryogenic suspension cells

text: suspSSHS3, proliferation library (embryogenic suspension cells cultivated in medium with 2,4-D)

characteristic: title

text: plasmid

text: pGEM-T Easy


text: live

text: Generic

text: Tenera Lab 221

text: Suppression subtractive hybridization library; cells cultured for 16 days in liquid pretreatment medium in the absence of 2,4-D (initiation conditions) were subtracted against cells cultured for 30 days in liquid proliferation medium containing 2,4-D (proliferation conditions).

characteristic: description

text: initiated to form somatic embryos

text: Electro ten-blue Stratagene

text: Elaeis guineensis

text: embryogenic suspension cells

text: suspSSHS4, initiation library (embryogenic suspension cells cultivated in medium without 2,4-D for 16 days)

text: plasmid

text: pGEM-T Easy


text: Aalborg University

text: live

text: Generic

text: Kuras

text: 8 Weeks

text: Solanum tuberosum

text: EcoRI

text: XhoI

text: tuber

characteristic: tissue-type

text: Kuras, 8 week minituber

text: Phage

text: Lambda ZAPII


text: National Institute of fruit Science

text: live

text: Generic

text: Miyagawa wase

characteristic: cultivar

text: English Name: Satsuma Mandarin

text: Citrus unshiu

text: Mandarin fruit


text: Universidade do Algarve

text: live

text: Generic

text: cDNA from poly-A mRNA, amplified by long-distance PCR. Directional cloning and sequencing from 5' end of inserts.

text: adult

text: E.coli DH5a electromax

text: Fucus serratus

text: vegetative

text: Fucus serratus heat shock/recovery library

text: Plasmid

text: pDNA-LIB

characteristic: vector


characteristic: External Id

text: Universidade do Algarve

text: live

text: Generic

text: adult

text: E.coli DH5a electromax

text: Fucus serratus

text: vegetative

text: Fucus serratus desiccation/rehydration library

text: Plasmid

text: pDNA-LIB


text: Universidade do Algarve

text: live

text: Generic

text: adult

characteristic: dev-stage

text: E.coli DH5a electromax

text: Fucus vesiculosus

text: vegetative

text: Fucus vesiculosus desiccation/rehydration library

text: Plasmid

text: pDNA-LIB


text: CNRS/EPHE/UPVD

characteristic: INSDC center name

text: live

text: Generic

text: whole body

characteristic: cell type

text: holobiont Pocillopora damicornis/Symbiodinium Spp.

text: Pocillopora damicornis/Symbiodinium spp. mixed library

text: Bleaching Induced Genes (BIG) SSH library

text: Plasmid

text: pCR4(r)-TOPO(r) (Invitrogen)


text: CNRS/EPHE/UPVD

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: whole body

text: Bleaching Repressed Genes (BRG) SSH library

text: Plasmid

text: pCR4(r)-TOPO(r) (Invitrogen)


text: INRA Rennes

text: live

characteristic: INSDC status

text: Generic

text: aphids inoculated on one-week old Vicia faba germinations under non sterile conditions; long photoperiod (16-hr light/8-hr dark at 18 c. Brain tissue was dissected free from the suboesophageal and thoracic ganglia.

text: adult

text: Acyrthosiphon pisum

text: SfiI A

text: SfiI B

text: parthenogenetic viviparous female

characteristic: sex

characteristic: strain

text: brain

text: Ap-BrVp

text: pDNR-LIB


text: INRA Rennes

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: aphids inoculated on one-week old Vicia faba germinations under non sterile conditions; short photoperiod (12-hr light/12-hr dark at 18 c. Brain tissue was dissected free from the suboesophageal and thoracic ganglia.

text: adult

text: Acyrthosiphon pisum

text: SfiI A

text: SfiI B

text: sexual oviparous female

text: brain

text: Ap-BrOvp

text: pDNR-LIB


text: Iwate University

text: live

text: Generic

text: Plants of Lotus japonicus Gifu B-129 were grown in a greenhouse condition. Sepal was removed from flower bud. The mRNA was isolated from flower bud

text: SOLR

characteristic: lab-host

text: Lotus japonicus

characteristic: organism

text: ecotype Gifu B-129

text: flower bud

text: Lotus japonicus Gifu B-129 flower bud cDNA

text: pBlueScript SK(-)


text: Iwate University

text: live

text: Generic

text: Plants of Lotus japonicus Gifu B-129 were grown in a greenhouse condition. Sepal was removed from flower bud. The mRNA was isolated from immature flower bud

characteristic: description

text: SOLR

text: Lotus japonicus

text: ecotype Gifu B-129

text: immature flower bud

text: Lotus japonicus immature flower bud cDNA

characteristic: title

text: pBlueScript SK(-)


text: Nippon Veterinary and Animal Science University

text: live

text: Generic

text: country: Japan: Ooita ; isolation_source: Ayu

characteristic: isolate

text: Aphanomyces piscicida

text: Aphanomyces piscicida isolate NJM9803


text: Iwate University

text: live

text: Generic

text: Koshihikari

characteristic: cultivar

text: Plants of Oryza sativa cv. Koshihikari were grown in a greenhouse condition

text: SOLR

text: Oryza sativa Japonica Group

text: EcoRI

text: EcoRI

text: Anther

characteristic: tissue-type

text: Oryza sativa anther cDNA

text: pBlueScript SK(-)

characteristic: vector


characteristic: External Id

text: Iwate University

characteristic: INSDC center name

text: live

text: Generic

text: Koshihikari

text: SOLR

text: Oryza sativa Japonica Group

text: EcoRI

text: EcoRI

text: Pistil

text: Oryza sativa pistil cDNA

text: pBlueScript SK(-)


text: Swedish University of Agricultural Sciences (SLU)

characteristic: INSDC first public

text: live

text: Generic

text: Westar

text: Three-week-old plants

characteristic: dev-stage

text: Brassica napus

text: Leaf

text: Brassica napus cv. Westar three-week-old plants cDNA-AFLP

text: pbluescript SK(+)


text: Tea Research Association

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Mature

characteristic: cell type

text: According to the instructions given in Clontech Manual on SMART cDNA Library Construction Kit Manual (No. 634901; Clontech Lab Inc, USA)

text: Leaf

characteristic: organ

text: Camellia sinensis var. sinensis

text: Plasmid

text: pTriplX


text: Tea Research Association

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Mature

text: Leaf

text: Camellia sinensis var. assamica

text: Plasmid

text: pTriplEx (Clontech)


text: Tea Research Association

text: live

text: Generic

text: Immature

text: According to the instructions given in Invitrogen Manual on GeneRacer Kit Manual (No. L1500-01; Invitrogen, Carlsbad, USA)

text: Leaf

text: Camellia sinensis var. sinensis

characteristic: organism

text: Plasmid

text: pCRBlunt (Invitrogen)


text: Hirosaki University

text: live

text: Generic

text: Malus prunifolia

text: phloem

text: laser-microdissected Malus phloem cDNA


text: National Institute of Agrobiological Sciences

text: live

text: Generic

text: Pyricularia grisea

characteristic: strain

text: Magnaporthe grisea Guy11 (NIAS)


text: Sanger Institute

text: live

text: Generic

text: This sequence is from a pool of 16 to 36 hours whole zebrafish embryo cDNA library constructed by Invitrogen (1600 Faraday Ave, Carlsbad, California 92008, USA). cDNA was oligo dT primed, enriched for full length sequences and normalized from 1mg of total RNA. NotI-EcoRI cut cDNA was ligated into pENTR222.f1(-) and sublcloned into a custom pbluescript2 sk+ vector with gateway adapters, with NotI at the 5' end and EcoRI at the3' end. For more information on clones please contact: Pr. Uwe Strahle (uwe.straehle@itg.fzk. de), Institute of Toxicology and Genetics, FZK, Postfach 3640, D-76021 Karlsruhe, Germany, tel: +49-7247-82-6233, fax: +49-7247-82-3354.

characteristic: description

text: 16 - 36 hrs

text: Escherichia coli

characteristic: lab-host

text: Danio rerio

text: NotI

text: EcoRI

characteristic: title

text: pbluescript2 sk+


text: Centre de Biotechnonolgie de Borj cedria

text: live

text: Generic

text: Razegui

characteristic: cultivar

text: SSH cDNA Library prepared from leaves of salt tolerant grapevine cultivar Razegui cultivated under 100mM salt stress. SSH clones were cloned in to pTPCR vector by T/A cloning method.

text: Adult plant

text: Vitis vinifera

text: Leaves

characteristic: tissue-type

text: Vitis vinifera Razegui SSH cDNA library

text: Plasmid

text: pTPCR


characteristic: External Id

text: Tea Research Association

text: live

text: Generic

text: Juvenile

text: According to the instructions given in Clontech Manual on PCR Select cDNA Subtraction Kit (Cat. No. 637401; Clontech Lab Inc, USA)

text: Leaf

text: Camellia sinensis var. sinensis

text: Plasmid

text: pGEM

characteristic: vector


text: Iowa State University

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: Library constructed from RNA obtained from the all internal organs, except brain, of juvenile fathead minnow (equal males and females) treated for 24 and 48h with 10 ug per gram body weight of lipopolysaccharide.

text: Pimephales promelas

text: pooled internal organs (without brain)

text: Fathead minnow lipopolysaccharide-stimulated library

text: pBK-CMV


text: Iowa State University

characteristic: INSDC last update

text: live

text: Generic

text: Library constructed from RNA obtained from the all internal organs, except brain, of juvenile fathead minnow (equal males and females) treated for 24 and 48h with 2 ug per gram body weight of polyinosin-polycytidylic acid.

text: Pimephales promelas

text: pooled internal organs (without brain)

text: Fathead minnow polyinosin-polycytidylic acid-stimulated libraries

text: pBK-CMV


text: Iowa State University

text: live

characteristic: INSDC status

text: Generic

text: Library constructed from RNA obtained from primary culture of fathead head kidney cells, stimulated for 24 hours with lipopolysaccharide (10 ug/ml).

text: Pimephales promelas

text: pooled internal organs (without brain)

text: Fathead minnow head kidney cell library

text: pBK-CMV


text: University of Wisconsin-Milwaukee

text: live

text: Generic

text: Head kidney cells

characteristic: cell type

text: Library constructed from RNA obtained from head kidney cells incubated in primary culture for 24 hours in the presence of lipopolysaccharide (10 ug/ml).

text: Pimephales promelas

text: Fathead minnow lipopolysaccharide-stimulated head kidney cell library

text: pBK-CMV


text: 1) Biomedical Research Institute 2) Mahidol University

text: live

text: Generic

text: Poly(A)+ RNA was isolated from the whole body of 5 hours Schistosoma mansoni post exposure Biomphalaria glabrata (BS-90) juvenile snails. First strand cDNA was synthesized by using oligo-dT15. To construct library, 1st strand cDNA was processed into all steps of suppression subtractive hybridization with Clonetech PCR-SelectTM cDNA Subtraction kit (BD Biosciences). Briefly, double strand cDNA from exposed BS-90 snail was digested with RsaI, and split into 2 groups for linking with either adaptor I or adaptor 2R. The ligated cDNAs were performed twice for subtraction hybridization reaction by annealing with excess cDNA from exposed-NMRI (susceptible strain of Biomphalaria glabrata) juvenile snails. The resistant specific enriched-cDNA, which contains different adaptors on each end was enriched by PCR amplification using adaptor-specific primers. Then, an amplification product was cloned into the TOPO-TA cloning vector (Invitrogen Life Technologies) and transformed into E.coli strain DH5. Random white colonies were individually screened by PCR using universal M13 primers to check for the presence and sizes of inserts before picking single colonies into 96-wells microtiter plates.

text: Juvenile

characteristic: dev-stage

text: Biomphalaria glabrata

text: Hermaphrodite

characteristic: sex

text: Biomphalaria glabrata resistant snail (BS-90): Schistosoma mansoni exposed-snail library

text: Plasmid


text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Poly(A)+ RNA was isolated from the whole body of 5 hours Schistosoma mansoni post exposure Biomphalaria glabrata (LAC2) juvenile snails. First strand cDNA was synthesized by using oligo-dT15. To construct library, 1st strand cDNA was processed into all steps of suppression subtractive hybridization with Clonetech PCR-SelectTM cDNA Subtraction kit (BD Biosciences). Briefly, double strand cDNA from exposed LAC2 snail was digested with RsaI, and split into 2 groups for linking with either adaptor I or adaptor 2R. The ligated cDNAs were performed twice for subtraction hybridization reaction by annealing with excess cDNA from exposed NRI (susceptible strain of Biomphalaria glabrata) juvenile snails. The resistant specific enriched-cDNA, which contains different adaptors on each end was enriched by PCR amplification using adaptor-specific primers. Then, an amplification product was cloned into the TOPO-TA cloning vector (Invitrogen Life Technologies) and transformed into E.coli strain DH5-alpha. Random white colonies were individually screened by PCR using universal M13 primers to check for the presence and sizes of inserts before picking single colonies into 96-wells microtiter plates.

text: Juvenile

text: Biomphalaria glabrata

text: Hermaphrodite

text: Biomphalaria glabrata partial resistant snail (LAC2): Schistosoma mansoni exposed-snail library

text: Plasmid


text: live

text: Generic

text: Poly(A)+ RNA was isolated from the whole body of 5 hours Schistosoma mansoni post exposure Biomphalaria glabrata (NMRI) juvenile snails. First strand cDNA was synthesized by using oligo-dT15. To construct library, 1st strand cDNA was processed into all steps of suppression subtractive hybridization with Clonetech PCR-SelectTM cDNA Subtraction kit (BD Biosciences). Briefly, double strand cDNA from exposed NMRI snail was digested with RsaI, and split into 2 groups for linking with either adaptor I or adaptor 2R. The ligated cDNAs were performed twice for subtraction hybridization reaction by annealing with excess cDNA from exposed BS-90 or LAC (resistant and partial resistant strains of Biomphalaria glabrata, respectively) juvenile snails. The susceptible specific enriched-cDNA, which contains different adaptors on each end was enriched by PCR amplification using adaptor-specific primers. Then, an amplification product was cloned into the TOPO-TA cloning vector (Invitrogen Life Technologies) and transformed into E.coli strain DH5-alpha. Random white colonies were individually screened by PCR using universal M13 primers to check for the presence and sizes of inserts before picking single colonies into 96-wells microtiter plates.

text: Juvenile

text: Biomphalaria glabrata

characteristic: organism

text: Hermaphrodite

text: NMRI

characteristic: strain

text: Biomphalaria glabrata susceptible snail (NMRI): Schistosoma mansoni exposed-snail library

text: Plasmid


text: Turku Centre for Biotechnology

text: live

text: Generic

text: cDNA library was constructed from total lichen tissue collected from the island of Kustavi in southern Finland. Lichen tissue was cleaned of resident invertebrates and was dried under laboratory conditions for 1 month. Tissue was wetted overnight prior to RNA isolation.

characteristic: description

text: Cladonia rangiferina

text: EcoRI

text: XhoI

text: total lichen tissue from cleaned podetia

text: development library - midi

text: phagemid

text: pBluescript SK(-)


text: Turku Centre for Biotechnology

text: live

text: Generic

text: Cladonia rangiferina

text: EcoRI

text: XhoI

text: development library - mini

characteristic: title

text: phagemid

text: pBluescript SK(-)


text: Departamento de Biologia, Universidade de Aveiro

text: live

text: Generic

text: Subtracted cDNA library

text: Liver

characteristic: organ

text: Anguilla anguilla

text: Liver

text: DMBA-exposed Anguilla anguilla liver, forward subtracted cDNA library

text: pGEM-T Easy (Promega)


text: live

text: Generic

text: Subtracted cDNA library

text: Liver

text: Anguilla anguilla

text: Liver

characteristic: tissue-type

text: DMBA-exposed Anguilla anguilla liver, reverse subtracted cDNA library

text: pGEM-T Easy (Promega)


characteristic: External Id

text: Chulalongkorn University

text: live

text: Generic

text: The cDNA library was generated as part of Penaeus monodon EST project. Total RNA was isolated from hemocyte of Penaeus monodon. The mRNA was further purified, reverse transcribed and cloned to Uni-ZAP XR. The cDNA library in the form of single-stranded was hybridized with cDNA inserts excised from a plasmid DNA preparation of library itself. The unbound single-stranded DNA was transformed into XL-Blue.

text: E.coli SOLR strain

characteristic: lab-host

text: Penaeus monodon

text: EcoR I

text: Xho I

text: Hemocyte

text: Penaeus monodon hemocyte normalized library

text: Phage

text: Uni-ZAP XR


text: National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency

characteristic: INSDC center name

text: live

text: Generic

text: The cDNA library was generated as part of Penaeus monodon EST project. Total RNA was isolated from gill of Penaeus monodon treated with heat shock at 35oC for 1 h. The mRNA was further purified, reverse transcribed and cloned to Uni-ZAP XR.

text: E.coli SOLR strain

text: Penaeus monodon

text: EcoR I

text: Xho I

text: Gill

text: Penaeus monodon heat-induced gill cDNA library

text: Phage

text: Uni-ZAP XR

characteristic: vector


text: Genoplante

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: columbia

characteristic: cultivar

text: Arabidopsis thaliana

text: cultured cells

text: A


text: Genoplante

text: live

characteristic: INSDC status

text: Generic

text: columbia

text: Arabidopsis thaliana

text: cultured cells

text: B


text: Genoplante

text: live

text: Generic

text: copain

text: Triticum aestivum

text: glume

text: FUSA


text: Genoplante

text: live

text: Generic

text: copain

text: Triticum aestivum

text: glume

text: FUZ


text: Tea Research Association / North Bengal Agricultural University

text: live

text: Generic

text: Mature

characteristic: cell type

text: According to the instructions given in Clontech Manual on PCR Select cDNA Subtraction Kit (Cat. No. 637401; Clontech Lab Inc, USA)

text: Root

text: Camellia sinensis var. assamica

text: Plasmid

text: pGEM


text: Tea Research Association

text: live

text: Generic

characteristic: NCBI submission model

text: Juvenile

text: According to the instructions given in Clontech Manual on SMART cDNA Library Construction Kit Manual (No. 634901; Clontech Lab Inc, USA)

text: Leaf

text: Camellia sinensis var. assamica

text: Plasmid

text: pTriplX


text: Liaoning Ocean and Fisheries Science Research Institute

text: live

text: Generic

characteristic: NCBI submission package

text: E. coli

text: Kidney

text: Mizuhopecten yessoensis

text: Sfi IA

text: Sfi IB

text: Kidney

text: Japanese scallop SMART cDNA library from kidney

text: pDNR-LIB


text: live

text: Generic

text: E. coli

text: Mantle

text: Mizuhopecten yessoensis

characteristic: organism

text: Sfi IA

text: Sfi IB

text: Mantle

text: Japanese scallop cDNA library from mantle

text: pDNR-LIB


text: Institut Universitaire Europeen de la Mer, Universite de Bretagne Occidentale

text: live

text: Generic

text: Abalones exposed to challenge

text: adults

characteristic: dev-stage

text: Haliotis tuberculata

text: muscle

text: subtracted libraries from abalones exposed to bacterial challenge


text: Washington State University

text: live

text: Generic

text: haustoria

text: haustoria

text: Puccinia striiformis f. sp. tritici

text: Sfi I

text: Sfi I

characteristic: strain

text: Puccinia striiformis f. sp. tritici haustoria cDNA library

text: pDNR-LIB


text: Tea Research Association

text: live

text: Generic

text: Mature

characteristic: description

text: Leaf

text: Camellia sinensis var. assamica

characteristic: title

text: Plasmid

text: pGEM-T


text: JT Biohistory Research Hall

text: live

text: Generic

text: late pupae

text: Papilio xuthus

text: female

characteristic: sex

text: fore-leg tarsus

characteristic: tissue-type

text: full-length enriched swallowtail butterfly cDNA library, late pupae fore-leg tarsus


characteristic: External Id

text: Lawrence Berkeley National Lab

text: live

text: Generic

text: Sequences of RNA Ligase Mediated Rapid Amplification of cDNA Ends (RLM-RACE) products representing 5' ends of selected transcripts. Total RNA isolated from mixed stage embryos of the 'iso-1' isogenic strain was provided by the laboratory of Peter Cherbas (University of Indiana, Bloomington). Gene-specific 5' RLM-RACE products were generated using the FirstChoice RLM-RACE procedure (Ambion). Normalized pools of RACE products were created and either cloned and sequenced with an ABI3730 or directly sequenced on a 454 Life Science sequencer. Vector sequences were removed and the RNA adapter sequence was used to determine the orientation of the clone and was removed from the sequence. Each sequence represents a potential transcription start site and is oriented along the direction of transcription. NOTE: clones are not preserved and are not available for distribution.

text: mixed-stage embryo

characteristic: lab-host

text: whole organism

characteristic: organ

text: Drosophila melanogaster

text: male and female

text: RP D melanogaster mixed embryo RACE from pooled PCR products


text: Institute of Molecular and Cell Biology, National Institutes of Advanced Industrial Science and Technology

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: 7-10 days

text: Daucus carota L. (Ri T-DNA transformed carrot root)

text: Rhizophagus intraradices

text: DAOM 181602

text: extraradical mycelium


text: INRA

characteristic: INSDC last update

text: live

text: Generic

text: West Virginia 106

characteristic: cultivar

text: 8 days post anthesis

text: Solanum lycopersicum var. cerasiforme

text: fruit

text: West Virginia 106 fruit 8 days post anthesis


text: Iwate University

text: live

characteristic: INSDC status

text: Generic

text: Brassica napus

text: globular embryo

text: Brassica napus globular embryo subtracted cDNA library


text: Indian Institute of Chemical Biology (A Unit of Council of Scientific and Industrial Research)

text: live

text: Generic

characteristic: NCBI submission model

text: Xanthi

text: Vegetative

text: Escherichia coli

text: Leaf

text: Nicotiana tabacum

text: EcoRI

text: EcoRI

text: Hermaphrodite

text: Leaf

text: CB_NPSSHcDNA Library

text: pGEM-T Easy

characteristic: vector


text: University of Innsbruck, Department of Ultrastructure

text: live

text: Generic

characteristic: NCBI submission package

text: adult

text: Isodiametra pulchra

characteristic: organism

text: XhoI

text: hermaphrodite

text: Cpu_aW_adult EST library_2004

text: Plasmid

text: pBluescript SK-


text: University of Adelaide

text: live

text: Generic

text: cDNA suppression subtraction library made from a highly boron tolerant non-agronomic barley, Sahara 3771.

text: Hordeum vulgare

text: Sahara 3771

characteristic: strain

text: Hordeum vulgare Sahara 3771 suppression subtraction library


text: Institute of Medicinal Plants Development, Peking Union Medical College, Chinese Academy of Medical Sciences

text: live

text: Generic

text: To investigate the complex molecular mechanism of storage root development of Rehmannia glutinosa, SSH was used to enrich development relate transcripts in developing stage of storage root of Rehmannia glutinosa. The cDNA library was generated by taking developing storage root cDNA as tester and adventitious root cDNA as driver.

characteristic: description

text: Developing storage root

characteristic: dev-stage

text: Rehmannia glutinosa

text: Storage root

text: Developing storage root specific subtracted cDNA library of Rehmannia glutinosa

text: pGEM T-easy Vector (Promega)


text: Kansas State University

text: live

text: Generic

text: two cDNA libraries constructed using plasmid and lambda vectors

text: fifth-instar larvae

text: E. coli

text: Ostrinia nubilalis

text: unsexed

characteristic: sex

text: laboratory strain

text: whole gut

text: libraries of whole guts from fifth-instar European corn borer larvae

characteristic: title

text: pPCR-XL-TOPO plasmid vector and lambda Uni-ZAP XR vector


characteristic: External Id

text: CINVESTAV Campus Guanajuato

text: live

text: Generic

text: Rio Fuego

text: 2,7,12 and 18 days post-infestation

text: leaves

text: Solanum lycopersicum

text: Tomato leaves infested with Bemisia tabaci Subtractive libraries


text: Australian National University

text: live

text: Generic

text: The library was constructed in Lambda Uni-ZAP XR (Stratagene) according to manufacturers specifications. Clones were recovered as inserts in pBluescript SK- and sequenced using the T3 primer.

text: Adult

text: Acropora millepora

text: EcoRI

text: XhoI

text: hermaphrodite

text: colony from Great Barrier Reef

text: Acropora millepora Adult cDNA library

text: plasmid

text: pBluescript SK-


text: Bioengineering College, Chongqing University

text: live

text: Generic

text: The subtractive hybridization protocol from BD Bioscience Clontech (Palo Alto, CA) has been used to generate this specific cDNA library. Tester cDNA was prepared from purified hyphal bodies in the haemolymph of insect, and driver cDNA generated during conidia germination and appressorium formation on locust wing. The resulting cDNAs were cloned into PMD-18T vector.

text: Metarhizium acridum

text: hyphal bodies

characteristic: tissue-type

text: hyphal body SSH Library in the haemolymph of insect


text: The J. Craig Venter Institute

text: live

text: Generic

text: Gifu

text: Total RNAs were pooled together. Double strand cDNA were synthesized from pooled RNA by Evrogen using SMART technology (http://www.evrogen.com/technologies/SMART.shtml). The prepared cDNA was normalized by cDNA denaturation/reassociation, treatment by duplex-specific nuclease (DSN) and amplification of normalized fraction by PCR. The normalized cDNA was then digested with SfiI, size-fractionated, directionally ligated into pDNR-LIB (Clontech) and electroporated into GC10 competent cells (Gene Choice). RNAs were provided by Michael Grusak, Diego Sanchez and Satoshi Tabata.

text: Lotus japonicus

text: SfiIA

text: SfiIB

text: Cotyledons (4 d), NaCl-treated shoots, untreated shoots, nodules (40 dpi), Fe-deficient roots and Zn-deficient roots

text: pDNR-LIB (Clontech)


text: The J. Craig Venter Institute

text: live

text: Generic

text: Total RNAs were pooled together. Double strand cDNA were synthesized from pooled RNA by Evrogen using SMART technology (http://www.evrogen.com/technologies/SMART.shtml). The prepared cDNA was normalized by cDNA denaturation/reassociation, treatment by duplex-specific nuclease (DSN) and amplification of normalized fraction by PCR. The normalized cDNA was then digested with SfiI, size-fractionated, directionally ligated into pDNR-LIB (Clontech) and electroporated into GC10 competent cells (Gene Choice). RNAs were provided by Ivone Torres-Jerez.

text: Lotus japonicus

text: SfiIA

text: SfiIB

text: Leaf blades, open flowers, petioles, pods (10 to 20 daf), seeds (10 to 16 daf) and stems

text: pDNR-LIB (Clontech)


text: The J. Craig Venter Institute

text: live

text: Generic

text: Total RNAs were pooled together. Double strand cDNA were synthesized from pooled RNA by Evrogen using SMART technology (http://www.evrogen.com/technologies/SMART.shtml). The prepared cDNA was normalized by cDNA denaturation/reassociation, treatment by duplex-specific nuclease (DSN) and amplification of normalized fraction by PCR. The normalized cDNA was then digested with SfiI, size-fractionated, directionally ligated into pDNR-LIB (Clontech) and electroporated into GC10 competent cells (Gene Choice). RNAs were provided by Michael Grusak and Ivone Torres-Jerez.

text: Lotus japonicus

text: SfiIA

text: SfiIB

text: Nodules (0hr and 21d), non-inoculated roots, Fe-deficient roots and Zn-deficient roots

text: pDNR-LIB (Clontech)


text: Kyoto University

text: live

text: Generic

text: Nanko

text: country: Japan ; lat_lon: 34 N 135 E

text: Prunus mume

text: lateral buds

text: Subtraction library (endodormant buds minus paradormant buds)


text: Kyoto University

text: live

text: Generic

text: Nanko

text: country: Japan ; lat_lon: 34 N 135 E

text: Prunus mume

text: lateral buds

text: Prunus mume lateral buds


text: Kyoto University

text: live

text: Generic

text: Nanko

text: country: Japan ; lat_lon: 34 N 135 E

text: Prunus mume

text: lateral buds

text: Subtraction library (endodormant buds minus ecodormant buds)


text: Inner Mongolia Institute of Agricultural and Husbandry Sciences

text: live

text: Generic

text: The cDNA sequences were differential fragments obtained from male sterile and male fertile flower buds segregated from dominant genic male sterile line (H919) of flax (Linum usitatissimum L.) by DD-PCR technique and verified by Northern hybridization. Differential fragments G-E07-100, G-E07-850 and G-E07-250 were specifically expressed in male sterile flower buds and differential fragment G-S274-250 in male fertile flower buds.

text: Linum usitatissimum

text: Linum usitatissimum flower buds DD-PCR


text: University of Victoria

text: live

text: Generic

text: Tissue contributors: Bob Devlin, Fisheries and Oceans Canada

text: brain, kidney, spleen

characteristic: organ

text: Salmo salar

text: mixed tissue

text: ssalrgg mixed_tissue full-length Salmo salar cDNA


text: University of Victoria

characteristic: INSDC center name

text: live

text: Generic

text: brain, kidney, spleen

text: Salmo salar

text: mixed tissue

text: ssalrgh mixed_tissue full-length Salmo salar cDNA


text: Beijing Institute of Microbiology and Epidemiology

characteristic: INSDC first public

text: live

text: Generic

text: DDRT-PCR

text: adult

text: Aedes albopictus

text: female

text: head, thorax and abdomen

text: adult mosquito female head, thorax and abdomen


text: Korea Forest Research Institute

characteristic: INSDC last update

text: live

text: Generic

text: Subtracted cDNA library generated by forward subtracting of transcripts of mannitol treated poplar cells.

text: JM109 strain

characteristic: lab-host

text: Populus alba x Populus glandulosa

text: EcoRI

text: Suspension cell

text: cDNA library from mannitol treated poplar cells

text: plasmid

text: pGEM-T easy


text: National Taiwan University

text: live

characteristic: INSDC status

text: Generic

text: WSSV-infected

text: Postlarvae (PL20)

text: E.coli XL1-blue

text: Penaeus monodon

text: EcoRI

text: XhoI

text: PmTwI

text: plasmid

text: pBluescript


text: National Taiwan University

text: live

text: Generic

characteristic: NCBI submission model

text: Normal, no WSSV-infected

text: Postlarvae (PL20)

text: E.coli XL1-blue

text: Penaeus monodon

text: EcoRI

text: XhoI

text: PmTwN

text: plasmid

text: pBluescript


text: Universita di Catania, Facolta di Agraria

text: live

text: Generic

text: Subtractive cDNA library was constructed using mRNAs isolated from mycelium of Trichoderma harzianum grown in the presence of Hg2+ as tester and mRNAs isolated from mycelium of Trichoderma harzianum grown in the presence of Cd2+ as driver

text: 3 days liquid culture in Czapek Dox medium

text: Trichoderma harzianum

text: SacII

text: SpeI

text: IMI 393899

text: mycelium

text: Trichoderma harzianum suppression subtractive hybridization (SSH) cDNA library

text: Plasmid

text: pGEM-T Vector

characteristic: vector


text: Ludwig-Maximilians-Universitaet Muenchen

text: live

text: Generic

characteristic: NCBI submission package

text: Specimen collected in British Columbia (Canada), reaggregated tissue stored in RNALater, mRNA purified using the Dynabeads mRNA Purification Kit (Invitrogen), cDNA libraries constructed using the Clone Minor Library construction Kit (Invitrogen, pDONR222), 5' sequencing on an ABI3730XL. Funded by the German Research Foundation (DFG) Priority Program SPP1174 'Deep Metazoan Phylogeny', Project WO896/6

text: reaggregate

text: Heterochone calyx

text: choanosome

text: Heterochone reaggregate DMP cDNA Library


text: live

text: Generic

text: Specimen collected at North Stradbroke Island (Australia), tissue stored in RNALater, mRNA purified using the Dynabeads mRNA Purification Kit (Invitrogen), cDNA libraries constructed using the Clone Minor Library construction Kit (Invitrogen, pDONR222), 5' sequencing on an ABI3730XL. Funded by the German Research Foundation (DFG) Priority Program SPP1174 'Deep Metazoan Phylogeny', Project WO896/6

text: Leucetta chagosensis

characteristic: organism

text: choanosome

text: Leucetta chagosensis DMP cDNA Library


text: Michigan State University

text: live

text: Generic

text: Mycelium

characteristic: cell type

text: Total RNA was harvested from 50 mg fresh tissue of treated and untreated young apothecia. mRNA was purified by using the MicroPoly(A):oligo(dT) m-RNA purification kit from Ambion Inc (Texas, USA). One microgram of total mRNA was used to generate complementary cDNAs using the oligo(dT)21 primer. The tester cDNAs (atrazine treated apothecia) were digested with RsaI restriction enzyme, and then ligated to two different right and left linkers. The 'driver' (untreated control apothecia) is hybridized to the 'tester' and used to subtract out non-differentially expressed transcripts according to the manufacturer. The subtracted library was then generated by cloning the unhybridized tester cDNAs into pGEM. The T7 primer used for DNA sequencing.

text: Two and three days post-apothecial germination

text: Sclerotinia sclerotiorum

text: Telemorph

characteristic: strain

text: Apothecium

text: Differentially upregulated genes in atrazine-treated germinated apothecia


text: Universidad de Oviedo

text: live

text: Generic

text: Needle

text: Pinus radiata

text: hermaphrodite

text: Needle

text: Differentially expressed genes during needle maturation

text: pGEM-T easy


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT)-primed cDNA and cloned into pBluescript KS+ (Stratagene, La Jolla, CA). Library constructed at The Scripps Research Institute (La Jolla, CA) and sequencing performed at DOE Joint Genome Institute (Walnut Creek, CA).

characteristic: description

text: adult

characteristic: dev-stage

text: Alvinella pompejana

text: NotI

text: posterior end

text: CAFZ Alvinella pompejana Incyte regular library RN03


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT)-primed cDNA, cloned into pBluescript KS+ (Stratagene, La Jolla, CA) and normalized. Library constructed at The Scripps Research Institute (La Jolla, CA) and sequencing performed at DOE Joint Genome Institute (Walnut Creek, CA).

text: adult

text: Alvinella pompejana

text: NotI

text: posterior end

text: CAGA Alvinella pompejana Normalized library RN04 posterior end

characteristic: title


characteristic: External Id

text: DOE Joint Genome Institute

text: live

text: Generic

text: adult

text: Alvinella pompejana

text: NotI

text: posterior end

text: CAGB Alvinella pompejana Normalized library RN05 posterior end


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT)-primed cDNA and cloned into the pMCL200_cdna vector. PolyA RNA was primed with oligo(dT) primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'-CGGACGCGTGGG) and digested with NotI. cDNA was size selected >2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pMCL200_cdna vector. Library was created and sequencing done by the DOE Joint Genome Institute (Walnut Creek, CA).

text: adult

text: Alvinella pompejana

text: SalI

text: NotI

text: whole

characteristic: tissue-type

text: CAWI Alvinella pompejana whole worm (H)


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT)-primed cDNA and cloned into the pMCL200_cdna vector. PolyA RNA was primed with oligo(dT) primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'-CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 0.6-1.2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pMCL200_cdna vector. Library was created and sequencing done by the DOE Joint Genome Institute (Walnut Creek, CA).

text: adult

text: Alvinella pompejana

text: SalI

text: NotI

text: whole

text: CAWN Alvinella pompejana whole worm (L)


text: DOE Joint Genome Institute

text: live

text: Generic

text: adult

text: Alvinella pompejana

text: SalI

text: NotI

text: whole

text: CAWO Alvinella pompejana whole worm (H) RCA inserts


text: DOE Joint Genome Institute

text: live

text: Generic

text: adult

text: Alvinella pompejana

text: SalI

text: NotI

text: whole

text: CAWP Alvinella pompejana whole worm (L) RCA inserts


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT)-primed cDNA and cloned into vector pDNR-LIB (Clontech Laboratories, Inc., Mountain View, CA) using the SMART system (BD Biosciences, San Jose, CA). To generate the first strand, polyA RNA was primed with an oligo(dT) primer (5'-ATTCTAGAGGCCGAGGCGGCCGACATG(T)30 VN-3', where V = A, G, or C). The second strand was synthesized by PCR using primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' followed by digestion of the SfiI sites. cDNA was size selected for >2kb using 1% agarose gel electrophoresis, then ligated into pDNR-LIB. Library was created and sequencing performed at DOE Joint Genome Institute (Walnut Creek, CA).

text: egg

text: Alvinella pompejana

text: SfiI A

text: SfiI B

text: eggs

text: CAYN Alvinella pompejana eggs1, eggs2, eggs3

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT)-primed cDNA and cloned into vector pDNR-LIB (Clontech Laboratories, Inc., Mountain View, CA) using the SMART system (BD Biosciences, San Jose, CA). To generate the first strand, polyA RNA was primed with an oligo(dT) primer (5'-ATTCTAGAGGCCGAGGCGGCCGACATG(T)30 VN-3', where V = A, G, or C). The second strand was synthesized by PCR using primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' followed by digestion of the SfiI sites. cDNA was size selected for 0.6-1.2kb using 1% agarose gel electrophoresis, then ligated into pDNR-LIB. Library was created and sequencing performed at DOE Joint Genome Institute (Walnut Creek, CA).

text: egg

text: Alvinella pompejana

text: SfiI A

text: SfiI B

text: eggs

text: CAYO Alvinella pompejana eggs1, eggs2, eggs3

text: pDNR-LIB


text: Ningxia University

text: live

text: Generic

text: isolated by transcript subtraction hybridization (TSH) which includes both rRNA and mRNA

text: Solanum tuberosum

text: stem

text: Solanum tuberosum stem-specific library


text: Wellcome Trust Sanger Institute

text: live

text: Generic

text: adult

text: Glossina morsitans morsitans

text: T.brucei infected salivary gland

text: T.brucei infected salivary gland


text: Central Institute of Medicinal and Aromatic Plants

text: live

text: Generic

text: Sampada

characteristic: cultivar

text: seedling

text: Papaver somniferum

text: subtracted cDNA library of wound inducible ESTs in P. somniferum


text: University of Veterinary Medicine Hannover

characteristic: INSDC center name

text: live

text: Generic

text: The subtracted library contains ESTs from infective third-stage larvae (iL3) that have been subtracted against in vitro percutaneously migrated larvae (pmL3) from the dog hookworm, Ancylostoma caninum. Subtraction was achieved using the Suppression Subtractive Hybridization (SSH) technique. Differentially transcribed sequences were verified by differential screening and Virtual Northern Blot using freshly isolated mRNA of the two larval populations. Differentially transcribed clones were sequenced in both directions and the aligned molecule was included in the subtracted library.

text: third-stage larvae

text: Ancylostoma caninum

text: not differentiated

characteristic: sex

text: whole larvae

text: A. caninum subtracted iL3 library

text: Plasmid


characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: The subtracted library contains ESTs from in vitro percutaneously migrated larvae (pmL3) subtracted against infective third-stage larvae (iL3) from the dog hookworm, Ancylostoma caninum. Subtraction was achieved using the Suppression Subtractive Hybridization (SSH) technique. Differentially transcribed sequences were verified by differential screening and Virtual Northern Blot using freshly isolated mRNA of the two larval populations. Differentially transcribed clones were sequenced in both directions and the aligned molecule was included in the subtracted library.

text: third-stage larvae

text: Ancylostoma caninum

text: not differentiated

text: whole larvae

text: A. caninum subtracted pmL3 library

text: Plasmid


text: Northwest Center for Biological Research

text: live

characteristic: INSDC status

text: Generic

text: wild

text: PCR amplification using cDNA template from Chiltepin (Capsicum annuum var. glabriusculum) in salt stress, using primers desing from Capsicum annuum var. annuum. and primers desing of select mRNA from a sustractive library from Capsicum annuum.

text: leaves

characteristic: organ

text: Capsicum annuum var. glabriusculum

text: leaves


text: College of Animal Science and Technology, SiChuan Agricultrual University

text: live

text: Generic

characteristic: NCBI submission model

text: hepatocyte

text: Reciprocal suppression subtractive hybridization cDNA libraries constructed in primary hepatocyte between Landes goose (Anser anser) and Sichuan white goose (Anser cygnoides)

characteristic: lab-host

text: liver

text: Anser anser

text: male

text: Landes goose

text: Landes goose primary hepatocyte Library

text: pBluescript-T

characteristic: vector


text: live

text: Generic

characteristic: NCBI submission package

text: hepatocyte

characteristic: cell type

text: liver

text: Anser cygnoides

characteristic: organism

text: male

text: Sichuan white goose

text: Sichuan white goose primary hepatocyte Library

text: pBluescript-T


text: live

text: Generic

text: head-kidney

text: Lateolabrax japonicus

text: kidney

text: Japanese sea bass head-kidney cDNA library

text: Lamda ZAPexpress


text: Bioengineering College, Chongqing University

text: live

text: Generic

text: The Creator SMART cDNA libarary construction kit from BD Bioscience Clontech (Palo Alto, CA), combined with TRIMMER-DIRECT cDNA Normalization kit (Evrogen, Moscow), were used to generate this cDNA library. The resulting cDNAs were cloned into pDNR-Lib vector.

characteristic: description

text: Mature adult

characteristic: dev-stage

text: Locusta migratoria

text: males

text: Fat Body and Hemocyte

text: Fat Body and Hemocyte of Locusta migratoria Naturally Infected by Metarhizium anisopliae var. acridum

text: pDNR-Lib


characteristic: External Id

text: Defence Agricultural Research Laboratory

text: live

text: Generic

text: Ladakh Ecotype

text: Cicer microphyllum

text: Leaf

text: Cicer microphyllum cold responsive cDNA subtraction Library

characteristic: title


text: College of Life Science, Xinyang Normal University

text: live

text: Generic

text: young leaves

text: Camellia sinensis

text: young leaves

characteristic: tissue-type

text: Differentially expressed cDNA libraries of tea plant responding to tea geometrid larvae (Ectropis Obliqua Prout) feeding


text: University of Tsukuba

characteristic: INSDC center name

text: live

text: Generic

text: Daucus carota

text: somatic embryo

text: Daucus carota somatic embryo genes expressed during stress induction


text: Huazhong Agricultural University

text: live

text: Generic

text: Cara Cara

characteristic: cultivar

text: Fruits from August to October, during the stage of rapid cell enlargement and changes in pulp color from slightly pink to dark pink. The cDNA library was constructed following the SMART IVTM cDNA Library Construction kit (Clontech, Palo Alto, CA, USA).

text: Citrus sinensis

text: Hermaphrodite

characteristic: sex

text: Fruit

text: 'Cara cara' navel orange fruit cDNA library

text: pDNR-LIB


text: Yildiz Technical University

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Gemlik

text: CloneMiner cDNA Library Construction Kit was used.

text: Olea europaea

text: leaves and fruits

text: cDNA library from Olive leaves and fruits

text: Plasmid


text: The Plant Biotechnology Institute, National Research Council Canada

text: live

characteristic: INSDC status

text: Generic

text: Westar

text: Brassica napus cv. Westar; cDNA library JKBNHS1; 24-epibrassinolide treated shoot meristems and leaf primordia from 14d old seedlings.

text: Brassica napus

text: Brassica napus JKBNHS1

text: plasmid


text: University Muenster

characteristic: INSDC secondary accession

text: live

text: Generic

characteristic: NCBI submission model

characteristic: SRA accession

text: country: Japan ; host: Oryza sativa

text: Fusarium fujikuroi

characteristic: strain

text: Gibberella fujikuroi IMI58289


text: Indian Institute of Chemical Biology (A Unit of Council of Scientific and Industrial Research)

text: live

text: Generic

characteristic: NCBI submission package

text: According to the instructions given in Clontech Manual on PCR Select cDNA Subtraction Kit (Cat. No. 637401; Clontech Lab Inc, USA)

text: Vegetative stage

text: Escherichia coli

characteristic: lab-host

text: Leaf

characteristic: organ

text: Sinopodophyllum hexandrum

text: EcoRI

text: EcoRI

text: Subtracted cDNA library of Podophyllum hexandrum Royle

text: pGEM-T Easy

characteristic: vector


characteristic: External Id

text: Reliance Life Sciences Pvt Ltd

text: live

text: Generic

text: Young 3-4 week old in vitro germinated Jatropha curcas were removed from the media, following which they were randomly separated into groups of 15-20 plantlets. Groups of these plants were placed in 150mM NaCl solution for 1.5-2.0 hours.Total RNA for cDNA synthesis was extracted from Jatropha curcas root samples using Qiagen RNA Miniprep Kit (Qiagen, Germany).Subsequent steps of cDNA synthesis and library construction were performed using Super SMART cDNA synthesis kit (clontech)

characteristic: description

text: 3-4 week old in vitro germinated plantlets

characteristic: dev-stage

text: Jatropha curcas

characteristic: organism

text: Pvu II

text: BstE II

text: Root

text: Expressed sequence tags from Jatropha curcas root cDNA library

text: Plasmid

text: pYES2.1 TOPO TA cloning vector


text: Center for Genetic Engineering and Biotechnology

characteristic: INSDC center name

text: live

text: Generic

text: Nicotiana megalosiphon

text: Root, Stem, Leaf

characteristic: tissue-type

text: Root, Stem, Leaf SSH cDNA Library

characteristic: title


text: Universidad de Oviedo

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Shoot apex subtractive library of PEG-stressed E. globulus versus non stressed.

text: Shoot

text: Eucalyptus globulus

text: hermaphrodite

characteristic: sex

text: C14 clone (ENCE)

text: Apex

text: Eucalyptus globulus C14 clone shoot apex under hydric stress

text: plasmid

text: pGEMTeasy


text: Universidade de Sao Paulo - USP

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: unidirectional library was constructed using long distance PCR (Smart cDNA kit) and Lambda TriplEx2 vector

text: adult

text: salivary glands

text: Amblyomma cajennense

text: female

text: cDNA library of salivary glands from feeding female tick SGFAc

text: Phage

text: Lambda TriplEx2


text: Universidade de Sao Paulo - USP

text: live

text: Generic

text: adult

text: salivary glands

text: Rhipicephalus microplus

text: female

text: cDNA library of salivary glands from feeding female tick SGFRm

text: Phage

text: Lambda TriplEx2


text: Universidade de Sao Paulo - USP

text: live

text: Generic

text: adult

text: salivary glands

text: Rhipicephalus sanguineus

text: female

text: cDNA library of salivary glands from feeding female tick SGFRs

text: Phage

text: Lambda TriplEx2


text: Penn State University

text: live

text: Generic

text: cDNA used for library prepared from healthy mature tree by random hexamer priming of mRNA

text: Fagus grandifolia

text: pooled sample from multiple tissues

text: American beech multiple tissues


text: National Research Centre for Banana, Trichy

text: live

text: Generic

text: Manoranjitham

characteristic: cultivar

text: SSH library was made using 3 months old banana cv. Manoranjitham (AAA) leaf as tester and cv. Robusta (AAA) leaf as driver to identify sigatoka resistance genes.

text: 3 months old tissue cultured plants

text: leaf

text: Musa acuminata AAA Group

text: Unisexual

text: Musa cv. Manoranjitham cDNA library

text: plasmid

text: pGEMT Easy


text: The Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences

text: live

text: Generic

characteristic: NCBI submission package

text: Total RNA was extracted from Huperzia serrata Leaf. The cDNA library was constructed from total RNA, using the Creator SMART cDNA library kit (Clontech), and the cDNA were cloned directionally into the pDNR-LIB plasmid.

text: adult

text: Huperzia serrata

text: Leaf

text: Huperzia serrata Leaf cDNA Library

text: Plasmid

text: pDNR-LIB


text: SCRI

text: live

text: Generic

text: mRNA was extracted from a bulk preparation of G. pallida J2s and converted to cDNA which was size selected and cloned into Lambda UniZap XR. Mass excision was used to generate a plasmid copy for sequencing.

text: Second stage Juvenile

text: Globodera pallida

text: EcoRI

text: XhoI

text: Lindley

characteristic: strain

text: Globodera pallida J2 cDNA library

text: Phage (excised plasmid)

text: Lambda UniZap XR


text: SCRI

text: live

text: Generic

text: mRNA was extracted from a bulk preparation of G. pallida parasitic stage nematodes collected 7 days post infection of susceptible Solanum tuberosum (cv Desiree). cDNA was generated and amplified using a SMART cDNA system and cloned into the pDNR Lib vector

text: Parasitic stage nematodes 7 days post infection

text: Globodera pallida

text: SfiI

text: SfiI

text: Lindley

text: Plasmid

text: pDNR Lib


text: Research Institute of Subtropical Forestry, China Academy of Forestry

text: live

text: Generic

text: Vernicia fordii

text: EcoRI

text: XhoI

text: endosperm

text: cDNA Library from endosperm of tung oil tree

text: Phage

text: pBluscript


text: Northwest A&F University

text: live

text: Generic

text: leaves

characteristic: cell type

text: Total RNA was extracted by Trizol, then reverse transcription was conducted by AMV reverse transcriptase; then double strand cDNA was synthesized and subjected to SSH analysis by using PCR Select cDNA Subtraction Kit (Clontech company). After twice hybridization the PCR products were purified and cloned into pGEM-T Easy vector and transformed to E coli strain DH5alpha, sequencing were conducted by Sp6 or T7 primer at LiCOR 4200 DNA Sequencer.

text: 4-5 leaves, after driver was inoculated Stripe rust pathogen CY32 in 3 days

text: Triticum aestivum

text: leaves

text: SSH-cDNA Library of stripe rust infection in leaves of stripe rust resistant material NR1121 compared with no infection


text: UAS, GKVK, Bangalore-560065

text: live

text: Generic

text: Total RNA was isolated from 4-week-old healthy mulberry leaves and reverse transcribed. Gene specific primer was used for PCR amplification and the amplified product was cloned into T/A cloning vector (pTZ57R/T).

text: 4 wk old leaves

text: Escherichia coli

characteristic: lab-host

text: Leaf

characteristic: organ

text: Morus indica

text: Hind III

text: Bisexual

text: Leaf

text: Complementary DNA of mulberry (Morus indica)

characteristic: vector


characteristic: External Id

text: The Breed & Biotechnology Key Laboratory of Sheep

text: live

text: Generic

text: gravid proglottid

characteristic: dev-stage

text: Moniezia expansa

text: Shihezi

text: Moniezia expansa Shihezi gravid proglottids


text: Centre de Biotechnonolgie de Borj cedria

characteristic: INSDC center name

text: live

text: Generic

text: Razegui

text: Proteomic analysis of Tunisian grapevine cultivar Razegui under salt stress.

characteristic: description

text: Adult plant

text: Vitis vinifera

characteristic: organism

text: Leaves

text: Vitis vinifera cv. Razegui two-dimensional electrophoresis

text: Plasmid

text: pGEM-T Easy vector (promega)


text: Wellcome Trust Sanger Institute

characteristic: INSDC first public

text: live

text: Generic

text: adult

text: Glossina morsitans morsitans

text: head

characteristic: tissue-type

text: T.brucei infected head

characteristic: title


text: Wellcome Trust Sanger Institute

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: adult

text: Glossina morsitans morsitans

text: reproductive organ

text: T. brucei infected reproductive organ


text: University of Florida/IFAS

text: live

text: Generic

characteristic: NCBI submission model

text: adult

text: Culex quinquefasciatus

text: female

characteristic: sex

text: midgut

text: Mosquito midgut 4 h after females are fed a West Nile virus-infected blood meal


text: Northwest A&F University

text: live

text: Generic

characteristic: NCBI submission package

text: uninucleate pollen grain stage

text: alabastrum

text: Capsicum annuum

text: alabastrum

text: Pepper cytoplasmic male sterility


text: National Institute of Horticultural & Herbal Science, RDA

text: live

text: Generic

text: Aori No. 9

characteristic: cultivar

text: Total RNA was extracted from pedicels of young fruit or flower using the 'pine tree' method. Poly-A mRNA was isolated from total RNA using Oligotex Direct mRNA kit (Qiagen). cDNA library was constructed with SMART cDNA library construction kit (Clontech) for sequencing.

text: Full bloom

text: DH10B Chloramphenicol resistant

text: Malus domestica

text: Lateral fruit pedicels

text: (ALFP) Apple lateral fruit pedicels cDNA library

text: pDNR-LIB


text: live

text: Generic

text: Suppressive-subtractive hybridisation (SSH) library.

text: Adult

text: Ascaris suum

text: Female

text: Ascaris suum female adult, gender-enriched SSH library


text: live

text: Generic

text: Adult

text: Ascaris suum

text: Male

text: Ascaris suum male adult, gender-enriched SSH library


text: University of Bristol

text: live

text: Generic

text: Mature pistils havested from S1S2 plants were pooled, and total RNA extracted. cDNA was prepared using the SMART PCR cDNA Synthesis kit (Clontech) and pistil-specific cDNA's were selected using the PCR-Select cDNA subtraction kit (Clontech). cDNA's were directionally cloned into pCR2.1-TOPO vector. clones were randomly picked based on blue/white selection with X-galactose. Clones were grown in 96-well microtitre plates containing LB broth and selective antibiotic and are maintained as glycerol stocks in E. coli TOPO10 host strain.

text: Mature

text: E. coli TOP10

characteristic: lab-host

text: Senecio squalidus

text: EcoRI

text: XhoI

text: Pistil

text: Plasmid


text: University of Bristol

text: live

text: Generic

text: Mature pistils havested from S1S3 plants were pooled, and total RNA extracted. cDNA was prepared using the SMART PCR cDNA Synthesis kit (Clontech) and pistil-specific cDNA's were selected using the PCR-Select cDNA subtraction kit (Clontech). cDNA's were directionally cloned into pCR2.1-TOPO vector. clones were randomly picked based on blue/white selection with X-galactose. Clones were grown in 96-well microtitre plates containing LB broth and selective antibiotic and are maintained as glycerol stocks in E. coli TOPO10 host strain.

text: Mature

text: E. coli TOP10

text: Senecio squalidus

text: EcoRI

text: XhoI

text: Pistil

text: Plasmid


text: University of Bristol

text: live

text: Generic

text: Mature pistils havested from S1S4 plants were pooled, and total RNA extracted. cDNA was prepared using the SMART PCR cDNA Synthesis kit (Clontech) and pistil-specific cDNA's were selected using the PCR-Select cDNA subtraction kit (Clontech). cDNA's were directionally cloned into pCR2.1-TOPO vector. clones were randomly picked based on blue/white selection with X-galactose. Clones were grown in 96-well microtitre plates containing LB broth and selective antibiotic and are maintained as glycerol stocks in E. coli TOPO10 host strain.

text: Mature

text: E. coli TOP10

text: Senecio squalidus

text: EcoRI

text: XhoI

text: Pistil

text: Plasmid


text: Universidade Federal do Para

text: live

text: Generic

text: SSH library was made using roots of Piper tuberculatum plants inoculated with Fusarium solani f. sp. piperis as tester and non-inoculated plants as driver to identify genes potentially related to Fusarium disease resistance.

text: Root

characteristic: organ

text: Piper tuberculatum

text: Piper tuberculatum cDNA library

text: Plasmid


text: Corporacion para Investigaciones Biologicas

text: live

text: Generic

text: Conidia to mycelium germination

text: Paracoccidioides brasiliensis

characteristic: strain

text: Conidia to mycelium germination EST Library


characteristic: External Id

text: Korea Polar Research Institute (KOPRI)

text: live

text: Generic

text: Stratagene's cDNA Synthesis Kit (catalog number 200401), ZAP Express Vector Kit (catalog number 239211), and Gigapack III Gold Packaging Extract (catalog number 200201) were used to construct the cDNA library. After second-strand cDNA synthesis, adapter ligation, and size fractionation, the column eluent was then ligated into Stratagene's UniZAP XR vector that has been digested with EcoRI and XhoI, and phosphorylated by Stratagene. The ligated DNA was packaged using Stratagene's Gigapack III Gold Packaging Extract and the pBluescript SK phagemid and insert was circularized to phagemid by in vivo excision.

text: Griffithsia okiensis

text: EcoRI

text: XhoI

text: Male gametophytes, female gametophytes, and tetrasporophytes

text: G. okiensis cDNA Library

text: pBluescript SK

characteristic: vector


text: National Diagnostics Centre

characteristic: INSDC center name

text: live

text: Generic

text: Suppression subtractive hybridization library

text: Nov vs Jul Mature Ovary

characteristic: dev-stage

text: Salmo salar

text: Ovary

text: Salmo salar Ovary SSH forward Nov vs Jul Mature Ovary


text: National Diagnostics Centre

text: live

text: Generic

characteristic: description

text: smolt april vs smolt may

text: Salmo salar

characteristic: organism

text: Pituitary

text: Salmo salar Pituitary SSH Forward smolt april vs smolt may


text: National Diagnostics Centre

characteristic: INSDC first public

text: live

text: Generic

text: smolt april vs sea cage smolt

text: Salmo salar

text: Pituitary

characteristic: tissue-type

text: Salmo salar Pituitary SSH Reverse smolt april vs sea cage smolt


text: National Diagnostics Centre

characteristic: INSDC last update

text: live

text: Generic

text: parr female vs Grilse female

text: Salmo salar

text: Pituitary

text: Salmo salar Pituitary SSH Forward parr female vs Grilse female

characteristic: title


text: National Institute of Radiological Sciences

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: synonym: Pseudokirchneriella subcapitata ; transcript-derived fragment up-regulated by ionizing radiation

text: Selenastrum capricornutum

text: ATCC 22662

text: Selenastrum capricornutum ATCC 22662 up-regulated by ionizing radiation


text: State University of Campinas - UNICAMP

text: live

text: Generic

characteristic: NCBI submission package

text: 45 days plantlets

text: Hymenaea courbaril var. stilbocarpa

text: Hymenaea courbaril mixed tissues


text: University of Georgia

text: live

text: Generic

text: Tifrunner

characteristic: cultivar

text: Total RNAs were isolated from leaves and cotyledons with Trizol (Invitrogen). The normalized cDNA libraries were constructed with Smart cDNA Library Construction Kit (Clontech). The inserts were cloned into pPCR-Script-Amp-SK+ vector after Srf I digestion. The clones were transformed to the host cell DH10B and selected using chloramphenicol.

text: cotyledon, young leaves

text: Arachis hypogaea

text: SrfI

text: SrfI

text: Normalized cDNA library from cotyledon and young leaves of peanut

text: pPCR-Script-Amp-SK+ (Strategene)


text: Rubber Research Institute of China

text: live

text: Generic

text: cDNA-AFLP fragment of rubber tree.

text: Hevea brasiliensis

text: Bark

text: Hevea brasiliensis TDF clone

text: pGEM-T


text: live

text: Generic

text: Genes expressed following cadmium exposure in the earthworm Eisenia fetida through DDRT-PCR protocol.

text: Eisenia fetida

text: Earthworm Eisenia fetida DDRT-PCR


text: Istituto Agrario di San Michele all'Adige (IASMA)

text: live

text: Generic

text: Grapevine cell cultures were collected 2 h after DIMEB treatment (50 mM) from control and treated samples. Total RNA from control and treated samples were reverse transcribed in double-stranded cDNA using the SMART PCR cDNA synthesis kit (Clontech Laboratories) as described by the manufacturer. A subtracted library was constructed using the suppression subtractive hybridization (SSH) technique performed with the PCR-Select cDNA Subtraction Kit (Clontech Laboratories) according to the manufacturer's procedure. In details, after adaptor ligation, tester cDNA from treated cells was hybridized with driver cDNA consisting of control cell cDNAs. Two rounds of hybridizations and PCR amplifications were performed to enrich in DIMEB-specific transcripts. The subtracted cDNAs amplified in the secondary PCR were cloned into the pCR 2.1-TOPO vector (Invitrogen) by means of TA cloning strategy and transformed in One Shot TOP10 Eletrocompetent E.coli (Invitrogen) cells. Positive transformants were grown at 37oC in 96-well plates containing LB medium supplemented with 50 mg/ml kanamycin and 10% glycerol and then stored at -80oC. To validate that the isolated clones were transcripts induced by DIMEB treatment, they were spotted on nylon membranes and hybridized with the same RNAs from control and treated cells used for SSH library construction. Starting from 384 clones, after screening by hybridization and sequencing, we obtained 168 high-quality sequences.

text: stationary phase

text: TOP10 E.coli (Invitrogen)

characteristic: lab-host

text: Vitis cinerea var. helleri x Vitis riparia

text: liquid cell culture

text: SSH cDNA library from DIMEB treated grapevine cell cultures

text: Plasmid

text: pCR 2.1-TOPO vector (Invitrogen)