text: INRA - SCRIBE

text: live

text: Generic

text: Clone distribution : AGENAE Resource centre. Francois PIUMI, Francois.Piumi@jouy.inra.fr, INRA, CEA Radiobiologie et Etude du genome (LREG), Domaine de Vilvert, 78352, Jouy-en-Josas cedex, FRANCE, +33 (0) 1.34.65.28.02, +33 (0) 1.34.65.22.73

characteristic: lab-host

text: Oncorhynchus mykiss

text: testis

text: AGENAE Rainbow trout normalized testis library (tcbi)


status: PUBLIC

characteristic: External Id

text: INRA - SCRIBE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: AGENAE Rainbow trout multi-tissues - normalized + 2 subtractions; Clone distribution : AGENAE Resource centre. Francois PIUMI, Francois.Piumi@jouy.inra.fr, INRA, CEA Radiobiologie et Etude du genome (LREG), Domaine de Vilvert, 78352, Jouy-en-Josas cedex, FRANCE, +33 (0) 1.34.65.28.02, +33 (0) 1.34.65.22.73

characteristic: description

text: from embryos to adults

tag: attribute

characteristic: dev-stage

text: Oncorhynchus mykiss

characteristic: organism

text: multi-tissues

characteristic: tissue-type

text: tcbk

characteristic: title

characteristic: vector


text: National Institute of Biology

text: live

text: Generic

text: Igor

characteristic: cultivar

text: Leaves

characteristic: organ

text: Solanum tuberosum

text: Potato subtractive library of down-regulated genes after PVYNTN infection

text: Plasmid

text: pGEM-TEasy


text: National Institute of Biology

text: live

text: Generic

text: Igor

text: Leaves

text: Solanum tuberosum

text: Potato subtractive library of up-regulated genes after PVYNTN infection

text: Plasmid

text: pGEM-TEasy


text: Langston University

text: live

text: Generic

text: Jamspan 90

text: cDNA library was constructed from peanut cultivar Jamspan 90. The leafs were collected from different plants, and placed into liquid N2 immediately and stored in -80 degree freezer. Total RNA was isolated with CONCERT Plant RNA Reagent(Invitrogen). mRNA was extracted and purified from total RNA with magnetic particle technology (NEB). cDNA synthesis and library construction followed the protocol developed by our center. The cDNA above 500bp were collected after size-fraction. The inserts were directionally cloned into pCMVSPORT 6.0 vector using EcoRV and NotI restriction sites (EcoRV site was destroyed after ligation of a cDNA). The un-amplified library was reached 14x106 cfu with 91% recombinant rate and average 1.4Kb insert size. The library was constructed by Dr. Ning Wu.

text: Unbloomed plant

text: Arachis hypogaea

text: EcoR V

text: Not I

text: Hermaphroidite

characteristic: sex

text: Jamspan 90

characteristic: strain

text: Leaf

text: Peanut Leaf cDNA library

text: Plasmid

text: pCMVSPROT 6.0


characteristic: External Id

text: ICAPB

text: live

text: Generic

text: Tardigrades (also know as water bears) are small free living animals inhabiting marine, fresh water and water film habitats. Hypsibius dujardini is a freshwater species maintained in lab culture (source strain Sciento Z151). The library was prepared from adults and juveniles and was constructed in pSPORT1 vector (Superscript, Invitrogen) using GeneRacer (Invitrogen) purified 5'capped mRNA by Jennifer Daub, University of Edinburgh. Sequencing was performed by Frances Thomas, University of Edinburgh.

text: mixed (adult and juvenile)

text: Hypsibius dujardini

text: Sal I (5'end)

text: Not I (3'end)

text: parthenogenic

text: whole body

text: Hypsibius dujardini mixed stage 5'capped (fraction 3)

text: plasmid


text: University of Edinburgh

characteristic: INSDC center name

text: live

text: Generic

text: mixed (adult and juvenile)

text: Hypsibius dujardini

text: Sal I (5'end)

text: Not I (3'end)

text: parthenogenic

text: whole body

text: Hypsibius dujardini mixed stage 5'capped (fraction 4)

text: plasmid


text: Chinese Academy of Sciences

characteristic: INSDC first public

text: live

text: Generic

text: morula stage embryo

text: Oryctolagus cuniculus

text: female

text: Oryctolagus cuniculus morula stage embryo


text: Japan Women's University

characteristic: INSDC last update

text: live

text: Generic

text: cDNA from 0, 2, 4, 8, 12, 24, 48, and 72h after the mixing of strain:NIES-67 and NIES-68 cells

text: sexual reproductive stage

text: Closterium peracerosum-strigosum-littorale complex sexual reproductive stage


text: Kobe University

text: live

characteristic: INSDC status

text: Generic

text: Iowa X469

text: Avena sativa

text: leaf

text: Avena sativa leaf 7-day-old


text: GR5064, Copenhagen University Hosp. Rigshospital

text: live

text: Generic

characteristic: NCBI submission model

text: carcinoma in situ (CIS)

characteristic: cell type

text: Homo sapiens

text: testis

text: Homo sapiens testis carcinoma in situ


text: live

text: Generic

characteristic: NCBI submission package

text: pGEM-TEasy vector, Promega (cat. #A1360)

characteristic: description

text: Capsicum annuum

text: Capsicum annuum pGEM-TEasy

text: pGEM-TEasy


text: University of Florida

text: live

text: Generic

text: MCC

text: Aplysia californica cDNA amplified library in pGemt

text: adult

characteristic: dev-stage

text: Aplysia californica

characteristic: organism

text: CNS

text: Aplysia californica MCC cell type cDNA amplified library

text: pGemt


text: National Institute on Aging/National Institutes of Health

text: live

text: Generic

text: Mouse cDNA project by the Laboratory of Genetics, National Institute on Aging (NIA), Intramural Research Program, NIH (http://lgsun.grc.nia.nih.gov/cDNA). This clone is among a rearrayed set of 11,424 clones from more than 20 cDNA libraries.

text: mixed

characteristic: lab-host

text: Mus musculus

text: SalI

text: NotI

text: mixed

text: NIA Mouse Unique Gene Set Version 2

characteristic: title

text: plasmid (ampicillin resistant)

text: pSPORT1 (Invitrogen)


text: University of Illinois, Urbana-Champaign

text: live

text: Generic

text: (1) The zmrsub1 library was created by subtracting the well-watered maize root library zmrww00 from the water-starved maize root libraries zmrws05 and zmrws48. The 3 starting libraries are described below in (3). (2) Purified plasmid preps from zmrws05 and zmrws48 were combined. Sequenced clones from the zmrww00 library were pooled and converted into single-stranded circles and used as a driver for subtraction. The PCR products from the driver were hybridized with the single-stranded version of the zmrws05-zmrws48 library for 88 hours at 30 oC. Unhybridized single-stranded DNA circles were separated from hybridized DNA rendered partially double-stranded and electroporated into DH110B cells to generate the subtracted library. The total number of clones was 2 x 106 cfu. Background of empty clones (detected by blue/white selection on agar plates) was approximately 10%. Insert size of the subtracted library, determined by PCR of the entire library, ranges from 0.5 to 2.1kb). (3) Root samples were collected in Robert E. Sharp's lab (University of Missouri-Columbia) to construct three normalized cDNA libraries. Dark-grown maize seedlings with primary roots 12-20 mm in length were transplanted to high (-0.03 MPa) or low water potential (-1.6 MPa) vermiculite, and harvested at 5 h and 48 h after transplanting. About 1,000 roots were used for each of the low water potential libraries (zmrws05 and zmrws48) while 500 roots were combined from each of the two time points at high water potential (zmrww00). Each root was divided into 4 segments (distances are from the junction of the root apex and root cap): segment 1, 0-3 mm plus the root cap; segment 2, 3-7 mm; segment 3, 7-12 mm; segment 4, 12-20 mm. (For detatils of conditions see (1) with nutrient modifications as in (2)). The three normalized cDNA libraries were constructed in the lab of Hans Bohnert (University of Illinois-UC). Total RNA was extracted by the 'hot Phenol' method (Plant Molecular Biology manual. D5: 1-13, 2nd ed., 1997). This method worked in eliminating carbohydrate material present in the root tips. The integrity of the RNA was verified by denaturing agarose gels and spectrophotometry (ratio A260/280). Poly(A)+mRNA was isolated twice from total RNA using the Oligotex Direct mRNA kit (Qiagen). Poly(A)+ mRNA was converted to double-stranded cDNA and tagged by using modified Oligo(dT) primers. One of 4 sequence tags corresponding to a different segment of the root was added to the 3'-end of the modified Oligo(dT) primers, including a Not1 site and used to reverse transcribe the segment-specific mRNAs into cDNAs. Each library contains all four tags. A suffix (s1, s2, s3, or s4) has been added to each sequence identifier to designate which region of the root (Root_segment_1, 2, 3, or 4) the sequence was found in based on the identification of the tag. A suffix of s0 indicates that the sequence tag, and hence the source segment, could not be identified. The double stranded cDNAs were size-selected (>450 bp). Size selected cDNAs were adaptored with EcoRI adaptors at both ends, and then digested with NotI. The cDNA was directionally cloned into EcoR1-NotI digested pBS II SK(+) phagemid vector (Stratagene) and electroporated into E.coli DH10B. The total number of white colony forming units (cfu) in the primary libraries before amplification was as follows: zmrws05: 3.37 x 106; zmrws48: 4.87 x 106; zmrww00: 3 x 106. The background of empty clones was less than 1%. Inserts ranged from ~0.5kb to >2.5 kb, as determined by PCR. Plasmid DNA from the primary libraries then was converted to single-stranded circles and used as a template for PCR amplification using the T7 and T3 priming sites that flank the cloned cDNA inserts. The purified PCR products, representing the entire cDNA population cloned in each library, were used as a driver for normalization. Hybridization between the single-stranded library and the PCR products was carried out for 44 hours at 30oC. Non-hybridized single-stranded DNA circles were separated from hybridized DNA rendered partially double-stranded and electroporated into DH10B. The total number of clones with insert was: zmrws05: 2.0x107; zmrws48: 4.2x107; zmrww00: 1.1x107. The background of empty clones was less than 2%. Insert size, determined by PCR of the entire library, ranged from 0.5kb to 2.5kb. (1) Sharp R E; Silk W K; Hsiao T C. Growth of the Maize Primary Root at Low Water Potentials I. Spatial Distribution of Expansive Growth. Plant Physiology (Rockville). 87(1). 1988. 50-57. (2) Spollen W G; LeNoble M E; Samuels T D; Bernstein N; Sharp R E. Abscisic acid accumulation maintains maize primary root elongation at low water potentials by restricting ethylene production. Plant Physiology (Rockville). 122(3). March, 2000. 967-976.

text: Zea mays


text: National Cancer Institute / NIH

text: live

text: Generic

text: 1st strand cDNA was primed with an oligo(dT) primer [GCGGCTGAAGACGGCCTATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded cDNA was ligated to a DraIII adaptor [GGCCUACUGG], digested and directionally cloned into distinct DraIII sites of the pME18S-FL3. Library was size selected for 1.0 kb, with a average insert size of ~1.2kb, and is not amplified. Library constructed by Yutaka Suzuki (University of Tokyo Institute of Medical Science). Custom primers recommended for sequencing: 5' end primer 5'-GGATGTTGCCTTTACTTCTA-3' and 3' end primer 5'-CGACCTGCAGCTCGAGCACA-3'. Note: This is a Zebrafish Gene Collection (ZGC) library

text: DH10B TonA

text: eye

text: Danio rerio

text: DraIII

text: DraIII

text: neural retina, retinal pigment epithelium, lens and overlying skin, pooled embryos

characteristic: tissue-type

text: phagemid

characteristic: vector


text: National Cancer Institute / NIH

text: live

text: Generic

text: RNA obtained from pooled lung tissue from a mix of male and female animals at 8 wk old. Tissues were snap-frozen and kept at -80C for two days before RNA extraction and purification (Tri-reagent method). cDNA was primed using oligo-dT primer: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3' and cloned into the EcoRV/NotI sites of pExpress-1. Size-selection >1.4kb resulted in an average insert size of 2.3 kb. This primary library is not normalized (normalized primary library is NIH_MGC_232) and was constructed by Express Genomics (Frederick, MD). Note: this is a NIH_MGC library.

text: DH10B TonA

text: lung

characteristic: organ

text: Rattus norvegicus

text: EcoRV

text: NotI

text: lung, pooled

text: plasmid


text: National Cancer Institute / NIH

text: live

text: Generic

text: RNA obtained from pooled heart tissue from a mix of male and female animals at 8 wk old. Tissues were snap-frozen and kept at -80C for two days before RNA extraction and purification (Tri-reagent method). cDNA was primed using oligo-dT primer: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3' and cloned into the EcoRV/NotI sites of pExpress-1. Size-selection >1.4kb resulted in an average insert size of 2 kb. This primary library is not normalized (normalized primary library is NIH_MGC_234) and was constructed by Express Genomics (Frederick, MD). Note: this is a NIH_MGC library.

text: DH10B TonA

text: heart

text: Rattus norvegicus

text: EcoRV

text: NotI

text: heart, pooled

text: plasmid


text: Iowa State University

text: live

text: Generic

text: gland

text: mixed parasitic juvenile

text: gland cell

text: Heterodera glycines

text: Gland Cell LD PCR cDNA Library

text: plasmid

text: pGEM-T Easy


text: Justus-Liebig-University Giessen

text: live

text: Generic

text: Blumeria hordei

text: Blumeria graminis f. sp. hordei library (Korell M)


text: The Natural History Museum

text: live

text: Generic

text: country: Spain:Balearic Islands, Mallorca, Esporles

text: adult

text: Timarcha balearica


text: live

text: Generic

text: Library zmrws055 consists of the same cDNA material as library zmrws05 (described below) but was sequenced from the 5 prime end. The sequence identifier uses the '.s0' suffix because the library tag was at the 3 prime end and thus not identified. Samples were collected in Robert E. Sharp's lab (University of Missouri-Columbia) to construct three normalized cDNA libraries. Dark-grown maize seedlings with primary roots 12-20 mm in length were transplanted to high (-0.03 MPa) or low water potential (-1.6 MPa) vermiculite, and harvested at 5 h and 48 h after transplanting. About 1,000 roots were used for each of the low water potential libraries (zmrws05 and zmrws48) while 500 roots were combined from each of the two time points at high water potential (zmrww00). Each root was divided into 4 segments (distances are from the junction of the root apex and root cap): segment 1, 0-3 mm plus the root cap; segment 2, 3-7 mm; segment 3, 7-12 mm; segment 4, 12-20 mm. (For detatils of conditions see (1) with nutrient modifications as in (2)). The three normalized cDNA libraries were constructed in the lab of Hans Bohnert (University of Illinois-UC). Total RNA was extracted by the hot Phenol method (Plant Molecular Biology manual. D5: 1-13, 2nd ed., 1997). This method worked in eliminating carbohydrate material present in the root tips. The integrity of the RNA was verified by denaturing agarose gels and spectrophotometry (ratio A260/280). Poly(A)+mRNA was isolated twice from total RNA using the Oligotex Direct mRNA kit (Qiagen). Poly(A)+ mRNA was converted to double-stranded cDNA and tagged by using modified Oligo(dT) primers. One of 4 sequence tags corresponding to a different segment of the root was added to the 3'-end of the modified Oligo(dT) primers, including a Not1 site and used to reverse transcribe the segment-specific mRNAs into cDNAs. Each library contains all four tags. A suffix (s1, s2, s3, or s4) has been added to each sequence identifier to designate which region of the root (Root_segment_1, 2, 3, or 4) the sequence was found in based on the identification of the tag. A suffix of s0 indicates that the sequence tag, and hence the source segment, could not be identified. The double stranded cDNAs were size-selected (>450 bp). Size selected cDNAs were adaptored with EcoRI adaptors at both ends, and then digested with NotI. The cDNA was directionally cloned into EcoR1-NotI digested pBS II SK(+) phagemid vector (Stratagene) and electroporated into E.coli DH10B. The total number of white colony forming units (cfu) in the primary libraries before amplification was as follows: zmrws05: 3.37 x 106; zmrws48: 4.87 x 106; zmrww00: 3 x 106. The background of empty clones was less than 1%. Inserts ranged from ~0.5kb to >2.5 kb, as determined by PCR. Plasmid DNA from the primary libraries then was converted to single-stranded circles and used as a template for PCR amplification using the T7 and T3 priming sites that flank the cloned cDNA inserts. The purified PCR products, representing the entire cDNA population cloned in each library, were used as a driver for normalization. Hybridization between the single-stranded library and the PCR products was carried out for 44 hours at 30oC. Non-hybridized single-stranded DNA circles were separated from hybridized DNA rendered partially double-stranded and electroporated into DH10B. The total number of clones with insert was: zmrws05: 2.0x107; zmrws48: 4.2x107; zmrww00: 1.1x107. The background of empty clones was less than 2%. Insert size, determined by PCR of the entire library, ranged from 0.5kb to 2.5kb. (1) Sharp R E; Silk W K; Hsiao T C. Growth of the Maize Primary Root at Low Water Potentials I. Spatial Distribution of Expansive Growth. Plant Physiology (Rockville). 87(1). 1988. 50-57. (2) Spollen W G; LeNoble M E; Samuels T D; Bernstein N; Sharp R E. Abscisic acid accumulation maintains maize primary root elongation at low water potentials by restricting ethylene production. Plant Physiology (Rockville). 122(3). March, 2000. 967-976.

text: Zea mays


text: live

text: Generic

text: Library zmrws485 consists of the same cDNA material as library zmrws48 (described below) but was sequenced from the 5 prime end. The sequence identifier uses the '.s0' suffix because the library tag was at the 3 prime end and thus not identified. Samples were collected in Robert E. Sharp's lab (University of Missouri-Columbia) to construct three normalized cDNA libraries. Dark-grown maize seedlings with primary roots 12-20 mm in length were transplanted to high (-0.03 MPa) or low water potential (-1.6 MPa) vermiculite, and harvested at 5 h and 48 h after transplanting. About 1,000 roots were used for each of the low water potential libraries (zmrws05 and zmrws48) while 500 roots were combined from each of the two time points at high water potential (zmrww00). Each root was divided into 4 segments (distances are from the junction of the root apex and root cap): segment 1, 0-3 mm plus the root cap; segment 2, 3-7 mm; segment 3, 7-12 mm; segment 4, 12-20 mm. (For detatils of conditions see (1) with nutrient modifications as in (2)). The three normalized cDNA libraries were constructed in the lab of Hans Bohnert (University of Illinois-UC). Total RNA was extracted by the hot Phenol method (Plant Molecular Biology manual. D5: 1-13, 2nd ed., 1997). This method worked in eliminating carbohydrate material present in the root tips. The integrity of the RNA was verified by denaturing agarose gels and spectrophotometry (ratio A260/280). Poly(A)+mRNA was isolated twice from total RNA using the Oligotex Direct mRNA kit (Qiagen). Poly(A)+ mRNA was converted to double-stranded cDNA and tagged by using modified Oligo(dT) primers. One of 4 sequence tags corresponding to a different segment of the root was added to the 3'-end of the modified Oligo(dT) primers, including a Not1 site and used to reverse transcribe the segment-specific mRNAs into cDNAs. Each library contains all four tags. A suffix (s1, s2, s3, or s4) has been added to each sequence identifier to designate which region of the root (Root_segment_1, 2, 3, or 4) the sequence was found in based on the identification of the tag. A suffix of s0 indicates that the sequence tag, and hence the source segment, could not be identified. The double stranded cDNAs were size-selected (>450 bp). Size selected cDNAs were adaptored with EcoRI adaptors at both ends, and then digested with NotI. The cDNA was directionally cloned into EcoR1-NotI digested pBS II SK(+) phagemid vector (Stratagene) and electroporated into E.coli DH10B. The total number of white colony forming units (cfu) in the primary libraries before amplification was as follows: zmrws05: 3.37 x 106; zmrws48: 4.87 x 106; zmrww00: 3 x 106. The background of empty clones was less than 1%. Inserts ranged from ~0.5kb to >2.5 kb, as determined by PCR. Plasmid DNA from the primary libraries then was converted to single-stranded circles and used as a template for PCR amplification using the T7 and T3 priming sites that flank the cloned cDNA inserts. The purified PCR products, representing the entire cDNA population cloned in each library, were used as a driver for normalization. Hybridization between the single-stranded library and the PCR products was carried out for 44 hours at 30oC. Non-hybridized single-stranded DNA circles were separated from hybridized DNA rendered partially double-stranded and electroporated into DH10B. The total number of clones with insert was: zmrws05: 2.0x107; zmrws48: 4.2x107; zmrww00: 1.1x107. The background of empty clones was less than 2%. Insert size, determined by PCR of the entire library, ranged from 0.5kb to 2.5kb. (1) Sharp R E; Silk W K; Hsiao T C. Growth of the Maize Primary Root at Low Water Potentials I. Spatial Distribution of Expansive Growth. Plant Physiology (Rockville). 87(1). 1988. 50-57. (2) Spollen W G; LeNoble M E; Samuels T D; Bernstein N; Sharp R E. Abscisic acid accumulation maintains maize primary root elongation at low water potentials by restricting ethylene production. Plant Physiology (Rockville). 122(3). March, 2000. 967-976.

text: Zea mays


text: live

text: Generic

text: Library zmrww005 consists of the same cDNA material as library zmrww00 (described below) but was sequenced from the 5 prime end. The sequence identifier uses the '.s0' suffix because the library tag was at the 3 prime end and thus not identified. Samples were collected in Robert E. Sharp's lab (University of Missouri-Columbia) to construct three normalized cDNA libraries. Dark-grown maize seedlings with primary roots 12-20 mm in length were transplanted to high (-0.03 MPa) or low water potential (-1.6 MPa) vermiculite, and harvested at 5 h and 48 h after transplanting. About 1,000 roots were used for each of the low water potential libraries (zmrws05 and zmrws48) while 500 roots were combined from each of the two time points at high water potential (zmrww00). Each root was divided into 4 segments (distances are from the junction of the root apex and root cap): segment 1, 0-3 mm plus the root cap; segment 2, 3-7 mm; segment 3, 7-12 mm; segment 4, 12-20 mm. (For detatils of conditions see (1) with nutrient modifications as in (2)). The three normalized cDNA libraries were constructed in the lab of Hans Bohnert (University of Illinois-UC). Total RNA was extracted by the hot Phenol method (Plant Molecular Biology manual. D5: 1-13, 2nd ed., 1997). This method worked in eliminating carbohydrate material present in the root tips. The integrity of the RNA was verified by denaturing agarose gels and spectrophotometry (ratio A260/280). Poly(A)+mRNA was isolated twice from total RNA using the Oligotex Direct mRNA kit (Qiagen). Poly(A)+ mRNA was converted to double-stranded cDNA and tagged by using modified Oligo(dT) primers. One of 4 sequence tags corresponding to a different segment of the root was added to the 3'-end of the modified Oligo(dT) primers, including a Not1 site and used to reverse transcribe the segment-specific mRNAs into cDNAs. Each library contains all four tags. A suffix (s1, s2, s3, or s4) has been added to each sequence identifier to designate which region of the root (Root_segment_1, 2, 3, or 4) the sequence was found in based on the identification of the tag. A suffix of s0 indicates that the sequence tag, and hence the source segment, could not be identified. The double stranded cDNAs were size-selected (>450 bp). Size selected cDNAs were adaptored with EcoRI adaptors at both ends, and then digested with NotI. The cDNA was directionally cloned into EcoR1-NotI digested pBS II SK(+) phagemid vector (Stratagene) and electroporated into E.coli DH10B. The total number of white colony forming units (cfu) in the primary libraries before amplification was as follows: zmrws05: 3.37 x 106; zmrws48: 4.87 x 106; zmrww00: 3 x 106. The background of empty clones was less than 1%. Inserts ranged from ~0.5kb to >2.5 kb, as determined by PCR. Plasmid DNA from the primary libraries then was converted to single-stranded circles and used as a template for PCR amplification using the T7 and T3 priming sites that flank the cloned cDNA inserts. The purified PCR products, representing the entire cDNA population cloned in each library, were used as a driver for normalization. Hybridization between the single-stranded library and the PCR products was carried out for 44 hours at 30oC. Non-hybridized single-stranded DNA circles were separated from hybridized DNA rendered partially double-stranded and electroporated into DH10B. The total number of clones with insert was: zmrws05: 2.0x107; zmrws48: 4.2x107; zmrww00: 1.1x107. The background of empty clones was less than 2%. Insert size, determined by PCR of the entire library, ranged from 0.5kb to 2.5kb. (1) Sharp R E; Silk W K; Hsiao T C. Growth of the Maize Primary Root at Low Water Potentials I. Spatial Distribution of Expansive Growth. Plant Physiology (Rockville). 87(1). 1988. 50-57. (2) Spollen W G; LeNoble M E; Samuels T D; Bernstein N; Sharp R E. Abscisic acid accumulation maintains maize primary root elongation at low water potentials by restricting ethylene production. Plant Physiology (Rockville). 122(3). March, 2000. 967-976.

text: Zea mays


text: Universita degli Studi dell'Insubria

text: live

text: Generic

text: Dicentrarchus labrax

text: adult liver

text: cDNA from Rt-PCR on Dicentrarchus labrax liver


text: live

text: Generic

text: Mouse cDNA project by the Laboratory of Genetics, National Institute on Aging (NIA), Intramural Research Program, NIH (http://lgsun.grc.nia.nih.gov/cDNA). This is a long-transcript enriched cDNA library (Ref. Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]). Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 1.8 ug of total RNA, treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-S. The products were purified by phenol/chloroform and Centricon 100. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pSPORT1 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 2.4 kb. The library was constructed by Yulan Piao (NIA)

text: Mus musculus

text: SalI

text: NotI

text: Male

characteristic: sex

characteristic: strain

text: NIA Mouse E12.5 Male Mesonephros/Gonad cDNA Library

text: plasmid (ampicillin resistant)

text: pSPORT1 (Invitrogen)


characteristic: External Id

text: University of Illinois at Urbana-Champaign

characteristic: INSDC center name

text: live

text: Generic

text: near-term

text: placenta

text: Bos taurus

text: NG01 rearrayed Soares normalized bovine placenta


text: National Institute of Agrobiological Sciences

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Nilaparvata lugens

text: female

text: NIAS-Nl-Izumo

text: thorax

text: Nilaparvata lugens thorax NIAS-Nl-Izumo


text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Nilaparvata lugens

text: male

text: NIAS-Nl-Izumo

text: abdomen

text: Nilaparvata lugens abdomen male NIAS-Nl-Izumo


text: live

text: Generic

characteristic: NCBI submission package

text: nymph

text: Nilaparvata lugens

text: NIAS-Nl-Izumo

text: Nilaparvata lugens NIAS-Nl-Izumo nymph


text: live

text: Generic

text: Nilaparvata lugens

text: female

text: NIAS-Nl-Izumo

text: abdomen

text: Nilaparvata lugens abdomen female NIAS-Nl-Izumo


text: live

text: Generic

text: Nilaparvata lugens

text: female

text: NIAS-Nl-Izumo

text: ovary

text: Nilaparvata lugens ovary NIAS-Nl-Izumo


text: Fukui Medical University

text: live

text: Generic

text: Ovarian granulosa cell

characteristic: cell type

text: genes in rat granulosa cells affected by low concentrations of 2,3,7,8-tetrachlorodibenzo-p-dioxin

characteristic: description

text: Rattus norvegicus

text: Rattus norvegicus Ovarian granulosa cell


text: live

text: Generic

text: adult

characteristic: dev-stage

text: Sus scrofa

characteristic: organism

text: liver

text: full-length enriched swine cDNA library, adult liver


text: live

text: Generic

text: adult

text: Sus scrofa

text: ovary

text: full-length enriched swine cDNA library, adult ovary

characteristic: title


text: Iowa State University

text: live

text: Generic

text: gland

text: mixed parasitic juvenile

text: gland cell

text: Heterodera glycines

text: Gland Cell Amplified cDNA Library

text: plasmid

text: pSportII


text: live

text: Generic

text: adult

text: Sus scrofa

text: peripheral blood lymphocyte

characteristic: tissue-type

text: full-length enriched swine cDNA library, adult peripheral blood lymphocyte


text: live

text: Generic

text: adult

text: Sus scrofa

text: spleen

text: full-length enriched swine cDNA library, adult spleen


text: live

text: Generic

text: adult

text: Sus scrofa

text: thymus

text: full-length enriched swine cDNA library, adult thymus


text: live

text: Generic

text: adult

text: Sus scrofa

text: uterus

text: full-length enriched swine cDNA library, adult uterus


text: live

text: Generic

text: adult

text: Sus scrofa

text: lung

text: full-length enriched swine cDNA library, adult lung


text: live

text: Generic

text: adult

text: Sus scrofa

text: periphral blood mononuclear cell

text: full-length enriched swine cDNA library, adult periphral blood mononuclear cell


text: Chinese Academy of Sciences

text: live

text: Generic

text: Oryza sativa

text: Oryza sativa library (Han B)


text: National Institute of Crop Science

text: live

text: Generic

text: Glycine max

text: flooded roots

text: Glycine max flooded roots


text: Avestha Gengraine Technologies Pvt. Ltd.

text: live

text: Generic

text: ELECTROMAX DH10B cells

characteristic: cell-line

text: Terminalia arjuna

text: Sal I

text: Not I

text: cDNA library from medicinal plant Terminalia arjuna mature leaf

text: pSPORT1 vector

characteristic: vector


text: Hindustan Lever Research Centre

text: live

text: Generic

text: Mouse skin melanoma

text: B16 F1

text: This clone was derived through randomly amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) analysis of B16 cells exposed to ultraviolet radiation (UVR). The DNA was cloned into pGEM-T vector.

text: Mus musculus

text: SacII

text: SpeI

text: Mouse skin melanoma RAPD PCR clone

text: Plasmid

text: pGEM-T


text: Hindustan Lever Research Centre

text: live

text: Generic

text: Human keratinocyte

text: HaCaT

text: Homo sapiens

text: SacII

text: SpeI

text: Human keratinocyte RAPD PCR clone

text: Plasmid

text: pGEM-T


text: Auburn University

text: live

text: Generic

text: Intestine

characteristic: organ

text: Ictalurus furcatus

text: NotI

text: SalI

text: Ictalurus furcatus intestine cDNA library

text: Phagemid


text: Auburn University

text: live

text: Generic

text: Gill

text: Ictalurus punctatus

text: NotI

text: SalI

text: Ictalurus punctatus gill cDNA library

text: Phagemid


text: Washington University School of Medicine

text: live

text: Generic

text: Cloned unidirectionally. Poly(A)+ RNA was concentrated and purified using Dynabeads (Dynal) and mRNA eluted for first strand synthesis. First strand cDNA was created using MMLV RT (Powerscript, Clontech) and primed with oligo(dT) with XhoI site (primer CDSIII/3-XhoI) and 5'SMART 'anchor added using chimeric DNA-RNA oligo (SMART-NotI-r-GGG).SMART-NotI-r-GGG was used in case a PCR using the SMART template was also desired. 15 PCR cycles were done using first strand and primers specific to SL1 leader sequence (SL1-NotI) and 3' end(XhoI-No-dT). Double stranded cDNA was digested using XhoI/NotI, fractioned on Chroma-spin 400 columns (Clontech) and ligated to digested pGEM-11zf(+) plasmid. Chemically competent cells were used as host cells.

characteristic: lab-host

text: Meloidogyne javanica

text: XhoI

text: NotI

text: whole organism

text: Meloidogyne javanica J2 SL1 pGEM

text: plasmid (ampicillin resistant)

text: pGEM-11zf(+) (Promega)


characteristic: External Id

text: live

text: Generic

text: The library was constructed by Claire Murphy and Dr. Makedonka Dautova (mdautova@watson.wustl.edu) at Washington University, St.Louis. Oligo (dT)-SL1 based library. Meloidogyne javanica J2 cDNA PCR products of size >400 nucleotides containing SL1 on the 5'end and oligo (dT) on the 3' end were non-directionally cloned into pCRII-TOPO(Invitrogen) following the Topo TA cloning protocol.Meloidogyne javanica J2 were provided by Dr. David Bird (david_bird@ncsu.edu) at North Carolina State University, Raleigh, NC.

text: Meloidogyne javanica

text: EcoRI

text: EcoRI

text: whole organism

text: Meloidogyne javanica J2 SL1 TOPO v1

text: plasmid (Kanamycin and Ampicillin resistant)

text: pCRII-TOPO(Invitrogen)


text: University of Antwerp

characteristic: INSDC center name

text: live

text: Generic

text: country: Belgium:Antwerp

text: Apodemus sylvaticus

text: lung

text: Apodemus sylvaticus lung


text: John Innes Centre

characteristic: INSDC first public

text: live

text: Generic

characteristic: isolate

text: Rhizophagus intraradices

text: Glomus intraradices isolate BEG195


text: Justus-Liebig-University Giessen

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Manchuria

characteristic: cultivar

text: 7-day old seedling

text: Hordeum vulgare

text: primary leaf

text: Hordeum vulgare cv. Manchuria primary leaf 7-day old seedling


text: National Institute of Agrobiological Sciences

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Nilaparvata lugens

text: male

characteristic: sex

text: NIAS-Nl-Izumo

characteristic: strain

text: mixture of head and thorax

text: Nilaparvata lugens mixture of male head and thorax


text: Tokyo University of Marine Science and Technology, Graduate School of Marine Science and Technology

text: live

text: Generic

text: adult

characteristic: dev-stage

text: Paralichthys olivaceus

characteristic: organism

text: kidney

text: Paralichthys olivaceus kidney adult

characteristic: title


text: Nanjing Medical University

text: live

text: Generic

text: We purified the cDNA fragment using QIAquick Gel Extraction Kit. The purified PCR product was cloned into pinpoint(TM) Xa-1 T-vector(promega). We sequenced it using PinPoint(TM) Vector Sequencing Primer.

characteristic: description

text: testis

text: Homo sapiens

text: male

text: testis

characteristic: tissue-type

text: Human male testis

text: pinpoint(TM) Xa-1 T-vector

characteristic: vector


characteristic: External Id

text: live

text: Generic

text: ELECTROMAX DH10B cells

characteristic: lab-host

text: Gymnema sylvestre

text: Sal I

text: Not I

text: cDNA library from medicinal plant Gymnema sylvestre

text: pSPORT1 vector


text: Harbin Institute of Technology

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: mycelium

text: Trichoderma lixii

text: Trichoderma harzianum mycelium cDNA library

text: pBLUESCRIPT II SK+


text: University of Freiburg

characteristic: INSDC last update

text: live

text: Generic

text: Physcomitrium patens

text: protonema


text: University of Freiburg

text: live

characteristic: INSDC status

text: Generic

text: Physcomitrium patens


text: University of Freiburg

text: live

text: Generic

text: Physcomitrium patens

text: pp 03 04; 2000.fas


text: University of Texas at El Paso

text: live

text: Generic

text: Sequenced from subtractive fragments cloned into InVitrogen's pCR 2.1 vector.

text: adult

text: liver

characteristic: organ

text: Pseudopleuronectes americanus

text: male

text: Pseudopleuronectes americanus liver

text: pCR 2.1


text: live

text: Generic

characteristic: NCBI submission model

text: ELECTROMAX DH10B cells

text: Gloriosa superba

text: Sal I

text: Not I

text: cDNA library from medicinal plant Gloriosa superba

text: pSPORT1 vector


text: URGV, CNRS / INRA

text: live

text: Generic

characteristic: NCBI submission package

text: ecotype: Columbia 0 ; country: France

text: 4-weeks old

text: Arabidopsis thaliana

text: aerial vegetative tissues

text: Arabidopsis thaliana aerial vegetative tissues 4-weeks old


text: University of Illinois at Urbana-Champaign

text: live

text: Generic

text: uncultured bacterium

text: black band disease bacterial mat infecting Diploria strigosa


text: Oklahoma State University

text: live

text: Generic

text: 5' end of cDNA cloned near EcoRI site of pCMVSport6.0 and 3' end cloned near HindIII site of pCMVSport6.0. Average length of insert is 1.49 kb

text: E. coli

text: Aspergillus nidulans

text: EcoRI

text: HindIII

text: FGSC C26

text: vegetative mycelia

text: Aspergillus nidulans negative subtraction hybridization polysaccharide related cDNA plasmid library


text: National Cancer Institute / NIH

text: live

text: Generic

text: RNA obtained from pooled lung tissue from a mix of male and female animals at 8 wk old. Tissues were snap-frozen and kept at -80C for two days before RNA extraction and purification (Tri-reagent method). cDNA was primed using oligo-dT primer: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3' and cloned into the EcoRV/NotI sites of pExpress-1. Size-selection >1.4kb resulted in an average insert size of 2.3 kb. This primary library is normalized (non-normalized primary library is NIH_MGC_231) and was constructed by Express Genomics (Frederick, MD). Note: this is a NIH_MGC library.

text: DH10B TonA

text: lung

text: Rattus norvegicus

text: EcoRV

text: NotI

text: lung, pooled

text: plasmid


text: National Cancer Institute / NIH

text: live

text: Generic

text: RNA obtained from pooled kidney tissue from a mix of male and female animals at 8 wk old. Tissues were snap-frozen and kept at -80C for two days before RNA extraction and purification (Tri-reagent method). cDNA was primed using oligo-dT primer: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3' and cloned into the EcoRV/NotI sites of pExpress-1. Size-selection >1.4kb resulted in an average insert size of 2.2 kb. This primary library is normalized (non-normalized primary library is NIH_MGC_235) and was constructed by Express Genomics (Frederick, MD). Note: this is a NIH_MGC library.

text: DH10B TonA

text: kidney

text: Rattus norvegicus

text: EcoRV

text: NotI

text: kidney, pooled

text: plasmid


text: National Cancer Institute / NIH

text: live

text: Generic

text: RNA obtained from pooled kidney tissue from a mix of male and female animals at 8 wk old. Tissues were snap-frozen before RNA extraction and purification (Tri-reagent method). cDNA was primed using oligo-dT primer: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3' and cloned into the EcoRV/NotI sites of pExpress-1. Size-selection >1.4kb resulted in an average insert size of 2.2 kb. This primary library is non-normalized (normalized primary library is NIH_MGC_236) and was constructed by Express Genomics (Frederick, MD). Note: this is a NIH_MGC library.

text: DH10B TonA

text: kidney

text: Rattus norvegicus

text: EcoRV

text: NotI

text: kidney, pooled

text: plasmid


text: National Cancer Institute / NIH

text: live

text: Generic

text: RNA obtained from testis tissue of 8 wk old animal. Tissues were snap-frozen and kept at -80C before RNA extraction and purification (Tri-reagent method). cDNA was primed using oligo-dT primer: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3' and cloned into the EcoRV/NotI sites of pExpress-1. Size-selection >1.4kb resulted in an average insert size of 2.4 kb. This primary library is not normalized (normalized primary library is NIH_MGC_238) and was constructed by Express Genomics (Frederick, MD)

text: DH10B TonA

text: testis

text: Rattus norvegicus

text: EcoRV

text: NotI

text: testis, pooled

text: plasmid


text: Temasek Life Sciences Laboratory

text: live

text: Generic

text: Acacia mangium Willd.; cDNA library, flower subtracted against vegetative phyllode

text: Flower

text: Acacia mangium

text: Acacia mangium flower subtracted cDNA library

text: pGEM-T easy, Promega


text: Marine Biological Laboratory

text: live

text: Generic

text: mature pediveliger larvae

text: Argopecten irradians

text: whole body

text: Bay Scallop mature pediveliger larvae ZAP Express Library

text: pBK-CMV


text: Marine Biological Laboratory

text: live

text: Generic

text: planktonic veliger larvae

text: Argopecten irradians

text: whole body

text: Bay Scallop planktonic veliger larvae ZAP Express Library

text: pBK-CMV


text: Marine Biological Laboratory

text: live

text: Generic

text: spat

text: Argopecten irradians

text: whole body

text: Bay Scallop spat whole body ZAP Express Library

text: pBK-CMV


text: CBMEG. Departamento de Genetica e Evolucao - Instituto de Biologia- UNICAMP

text: live

text: Generic

text: H14954-29 (C.racemosa X C.arabica) x C.arabica breeding program

characteristic: cultivar

text: 60 young leaves from highly susceptible plants (S) and 60 leaves from highly resistant plants (R) were detached from the corresponding plants. Each leaf was inserted by the petiole into a 2 ml microcentrifuge tube filled with water. The tubes were then fixed to a plastic support and 40 S and 40 R leaves were placed in L. coffella rearing cages. The other support containing 20 S and 20 R leaves was incubated in a plastic box without coffee leaf miners. Cork bores 3 - 8 cm in diameter were used to collect leaf discs with eggs or with mines on the leaf surface. After the appropriate time points leaf discs were immediately frozen in liquid nitrogen. Afterwards, RNA was isolated and cDNA was synthesized.

text: Coffea hybrid cultivar

text: leaf

text: Coffee leaves infested by leaf miner subtraction library

text: plasmid

text: pBluescript SKII -


text: Southwest Agricultural University

text: live

text: Generic

text: Embryo (72 hours)

text: Bombyx mori

text: mixed

characteristic: sex

characteristic: strain

text: swj

text: pBluescript II SK(+)


text: Southwest Agricultural University

text: live

text: Generic

text: Embryo (nondiapause)

characteristic: dev-stage

text: Bombyx mori

characteristic: organism

text: mixed

text: swk

text: pBluescript II SK(+)


text: Southwest Agricultural University

text: live

text: Generic

text: Embryo (unfertilized)

text: Bombyx mori

text: mixed

text: swp

characteristic: title

text: pBluescript II SK(+)


text: Southwest Agricultural University

text: live

text: Generic

text: 5th-instar day-3 larva

text: Bombyx mori

text: female

text: Fat body

characteristic: tissue-type

text: swc

text: pBluescript II SK(+)


text: Southwest Agricultural University

text: live

text: Generic

text: 5th-instar day-3 larva

text: Bombyx mori

text: male

text: Fat body

text: swd

text: pBluescript II SK(+)

characteristic: vector


characteristic: External Id

text: Southwest Agricultural University

text: live

text: Generic

text: Bombyx mori

text: mixed

text: Fat body (pupa)

text: swl

text: pBluescript II SK(+)


text: Southwest Agricultural University

text: live

text: Generic

text: Hemocyte

characteristic: cell type

text: 5th-instar day-3 larva

text: Bombyx mori

text: female

text: swe

text: pBluescript II SK(+)


text: Southwest Agricultural University

characteristic: INSDC center name

text: live

text: Generic

text: Hemocyte

text: 5th-instar day-3 larva

text: Bombyx mori

text: male

text: swf

text: pBluescript II SK(+)


text: Southwest Agricultural University

characteristic: INSDC first public

text: live

text: Generic

text: 5th-instar day-3 larva

text: Bombyx mori

text: mixed

text: midgut

text: swb

text: pBluescript II SK(+)


text: Southwest Agricultural University

characteristic: INSDC last update

text: live

text: Generic

text: 5th-instar day-3 larva

text: Bombyx mori

text: female

text: ovary

text: swh

text: pBluescript II SK(+)


text: Southwest Agricultural University

text: live

characteristic: INSDC status

text: Generic

text: 5th-instar day-3 larva

text: Bombyx mori

text: mixed

text: silk gland

text: swa

text: pBluescript II SK(+)


text: Southwest Agricultural University

text: live

text: Generic

text: 5th-instar day-3 larva

text: Bombyx mori

text: male

text: testis

text: swg

text: pBluescript II SK(+)


text: Medical University of South Carolina

text: live

text: Generic

text: hemocytes

text: Penaeus vannamei

text: EcoRI

text: EcoRI

text: Plasmid


text: Medical University of South Carolina

text: live

text: Generic

text: gills

text: Penaeus vannamei

text: EcoRI

text: EcoRI

text: Plasmid


text: Medical University of South Carolina

text: live

text: Generic

text: hepatopancreas

text: Penaeus vannamei

text: EcoRI

text: EcoRI

text: HPCdWt

text: Plasmid


text: Medical University of South Carolina

text: live

text: Generic

text: gills

text: Penaeus vannamei

text: EcoRI

text: EcoRI

text: gCdWt

text: Plasmid


text: Medical University of South Carolina

text: live

text: Generic

text: gills

text: Penaeus vannamei

text: EcoRI

text: EcoRI

text: gWdCt

text: Plasmid


text: Medical University of South Carolina

text: live

text: Generic

text: hepatopancreas

text: Penaeus vannamei

text: EcoRI

text: EcoRI

text: HPWdCt

text: Plasmid


text: Medical University of South Carolina

text: live

text: Generic

text: hemocytes

text: Penaeus vannamei

text: EcoRI

text: EcoRI

text: hCdWt

text: Plasmid


text: Medical University of South Carolina

text: live

text: Generic

text: hemocytes

text: Penaeus vannamei

text: EcoRI

text: EcoRI

text: hWdCt

text: Plasmid


text: Medical University of South Carolina

text: live

text: Generic

text: hepatopancreas

text: Penaeus vannamei

text: EcoRI

text: EcoRI

text: Plasmid


text: University of Maryland School of Medicine

text: live

text: Generic

text: Plasmodium yoelii liver stage parasites were harvested by laser capture microdissection, from infected Balb/c mouse liver cryosections, 40 hours after inoculation with sporozoites. Total RNA was then isolated using the StrataPrep Total RNA microprep Kit from Stratagene.

characteristic: description

text: 40 hour liver schizont

text: Plasmodium yoelii

text: 17X NL

text: Plasmodium yoelii liver stage LCM cDNA library

text: plasmid


text: Dana Farber Cancer Institute

text: live

text: Generic

characteristic: NCBI submission model

text: For the purpose of protein interaction mapping, we generated a C. elegans cDNA library (AD-wrmcDNA) in which poly(dT)-primed reverse transcribed cDNA are fused to the AD-encoding sequence of the yeast transcription factor GAL4. This library was made with poly(A)+ RNA isolated from mated populations of wild-type (N2 strain) animals of all stages of development including embryonic, larval (L1 to L4 stages), adults and dauer. Approximately equal quantities of RNA from different populations were acquiered. cDNAs were generated and cloned into the two hybrid vector pPC86. The library contains ~3*10e7 clones. Reference - GATEWAY recombinational cloning: application to the cloning of large numbers of open reading frames or ORFeomes - Walhout AJ, Temple GF, Brasch MA, Hartley JL, Lorson MA, van den Heuvel S, Vidal M - Methods Enzymol. 2000;328:575-92

text: embryos, L1, L2, L3, L4, adult, dauer

text: Caenorhabditis elegans

text: male, hermaphrodite

text: AD-wrmcDNA library


text: Dana Farber Cancer Institute

text: live

text: Generic

characteristic: NCBI submission package

text: For the purpose of protein interaction mapping, predicted protein-encoding ORFs were amplified by PCR precisely between the predicted (WS9 version of WormPep) initiation and termination codons, using a cDNA library (AD-wrmcDNA library - Walhout etal. Methods Enzymol. 2000;328:575-92) as template. The resulting 11,984 Gateway cloned ORFs along with the attempted ones were transfered into a two-hybrid Destination vector downstream of the vector sequence encoding the activation domain (AD) of the yeast GAL4 transcription factor. Those constructs were pooled together to constitute a 'normalized' AD-ORFeome1.1 library. Reference - Reboul J, Vaglio P etal C. elegans ORFeome version 1.1: experimental verification of the genome annotation and resource for proteome-scale protein expression. Nat Genet. 2003 May;34(1):35-41. PMID: 12679813)

text: embryos, L1, L2, L3, L4, adult, dauer

text: Caenorhabditis elegans

text: male, hermaphrodite

text: AD-ORFeome1.0 library


text: GR5064, Copenhagen University Hosp. Rigshospital

text: live

text: Generic

text: Mus musculus

text: testis

text: Mus musculus testis C3H/He


text: Max-Planck-Institute for Terrestrial Microbiology

text: live

text: Generic

text: BEG 9

characteristic: isolate

text: Gigaspora rosea

text: presymbiotic hyphae

text: Gigaspora rosea BEG 9 presymbiotic hyphae


text: Medical University of South Carolina

text: live

text: Generic

text: hepatopancreas

text: Penaeus vannamei

text: EcoRI

text: EcoRI

text: HPCdit

text: Plasmid


text: Medical University of South Carolina

text: live

text: Generic

text: hepatopancreas

text: Penaeus vannamei

text: EcoRI

text: EcoRI

text: HPidCt

text: Plasmid


text: Medical University of South Carolina

text: live

text: Generic

text: hemocytes

text: Penaeus vannamei

text: EcoRI

text: EcoRI

text: hemCdit

text: Plasmid


text: Medical University of South Carolina

text: live

text: Generic

text: hemocytes

text: Penaeus vannamei

text: EcoRI

text: EcoRI

text: Plasmid


text: Medical University of South Carolina

text: live

text: Generic

text: gills

text: Penaeus vannamei

text: EcoRI

text: EcoRI

text: gillCdit

text: Plasmid


text: Medical University of South Carolina

text: live

text: Generic

text: hemocytes

text: Penaeus vannamei

text: Sfi

text: Sfi

text: Plasmid

text: pTriplEX


text: National Cancer Institute / NIH

text: live

text: Generic

text: RNA obtained from testis tissue of 8 wk old animal. Tissues were snap-frozen and kept at -80C before RNA extraction and purification (Tri-reagent method). cDNA was primed using oligo-dT primer: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3' and cloned into the EcoRV/NotI sites of pExpress-1. Size-selection >1.4kb resulted in an average insert size of 1.9 kb. This primary library is normalized (non-normalized primary library is NIH_MGC_237) and was constructed by Express Genomics (Frederick, MD)

text: DH10B TonA

characteristic: lab-host

text: testis

characteristic: organ

text: Rattus norvegicus

text: EcoRV

text: NotI

text: testis, pooled

text: plasmid


text: National Cancer Institute / NIH

text: live

text: Generic

text: RNA obtained from pooled heart tissue from a mix of male and female animals at 8 wk old. Tissues were snap-frozen and kept at -80C for two days before RNA extraction and purification (Tri-reagent method). cDNA was primed using oligo-dT primer: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3' and cloned into the EcoRV/NotI sites of pExpress-1. Size-selection >1.4kb resulted in an average insert size of 2.2 kb. This primary library is normalized (non-normalized primary library is NIH_MGC_233) and was constructed by Express Genomics (Frederick, MD). Note: this is a NIH_MGC library.

text: DH10B TonA

text: heart

text: Rattus norvegicus

text: EcoRV

text: NotI

text: heart, pooled

text: plasmid


text: Texas A&M University

text: live

text: Generic

text: The subtractive cDNA library, consisting of both forward and reverse subtractions, was prepared from polyA+ RNA from cowpea bruchid guts adapted as well as unadapted by soyacystatin N, respectively. The clontech PCR-select cDNA subtraction kit protocol was followed in library construction.

text: 4th instar

characteristic: dev-stage

text: E. coli

text: Callosobruchus maculatus

text: gut

text: Subtractive cDNA library from cowpea bruchid adapted by soyacystatin N

text: Plasmid

text: pCRII-TOPO


text: Laval University

text: live

text: Generic

text: Please see http://web.ahc.umn.edu/biodata/nsfpine/ for further information.

text: late wood

text: Pinus taeda

characteristic: organism

text: Coastal plain loblolly pine from North Carolina

characteristic: strain

text: primary xylem

text: Nsf Xylem Late wood Vertical


text: Institut National de la Recherche Agronomique

text: live

text: Generic

text: tissues: adipose tissue, brain, kidney, liver, muscle, ovary, testis, heart, hypothalamus, pancreas, skin, spleen, thymus, placenta, pituitary gland, seminal vesicle, small intestine, uterus, adrenals, bulbo uretral gland, cerebral trunk, epididymis, female gonad, gall-bladder, hippocampus, large intestine, male gonad, melanocytes, stomach, udder

text: from embryos to adults

text: Sus scrofa

text: mixed

characteristic: tissue-type

text: Sus Scrofa library (scan)

characteristic: title


characteristic: External Id

text: Laval University

text: live

text: Generic

text: compression

text: juvenile

text: Pinus taeda

text: primary xylem

text: Nsf Xylem Compression wood Inclined


text: Laval University

text: live

text: Generic

text: Planings (secondary)

characteristic: cell type

text: Transitional

text: Pinus taeda

text: Xylem

text: Nsf Xylem Planings wood Vertical


text: Laval University

characteristic: INSDC center name

text: live

text: Generic

text: Root (primary)

text: Transitional

text: Pinus taeda

text: Xylem

text: Nsf Xylem Root wood Vertical


text: University of Missouri

characteristic: INSDC first public

text: live

text: Generic

text: Williams 82

characteristic: cultivar

text: cDNA clones generated from soybean root hair tissue treated with Bradyrhizobium japonicum for 6 hours.

text: root hairs

text: Glycine max

text: root hairs

text: Soybean root hair subtracted cDNA library gmrhRww6

text: pGEM-T-Easy

characteristic: vector


text: Yonsei University

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Differentially expressed genes were isolated using suppression subtractive hybridization with cDNAs derived from cells at 0 min and 40 min after initiation of differentiation

characteristic: description

text: Naegleria gruberi

text: Naegleria gruberi subtractive hybridization library

text: plasmid

text: pCR 2.1-TOPO (Invitrogen)


text: University of Delaware

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Macrophage

text: Gallus gallus

text: EcoRI

text: XhoI

text: Female and male combined

characteristic: sex

text: Broiler

text: Blood

text: Unstimulated Chicken PBL Macrophage

text: Plasmid

text: pBluescript sk+


text: University of Delaware

text: live

text: Generic

text: Macrophage

text: Gallus gallus

text: EcoRI

text: XhoI

text: Female and male combined

text: Broiler

text: Blood

text: Interferon Stimulated Chicken PBL Macrophage

text: Plasmid

text: pBluescript sk+


text: University of Delaware

text: live

text: Generic

text: Macrophage

text: Gallus gallus

text: EcoRI

text: XhoI

text: Female and male combined

text: Broiler

text: Blood

text: LPS stimulated Chicken PBL Macrophage

text: Plasmid

text: pBluescript sk+


text: Weizmann Institute of Science

text: live

text: Generic

text: Suppression Subtractive Hybridization library of nitrogen starved (NS) Fusarium.

text: Fusarium oxysporum f. sp. lycopersici

text: SacII

text: SpeI

text: race 2

text: Plasmid

text: pGEM T


text: National Eye Institute

text: live

text: Generic

text: 64ug total RNA was extracted from 200 adult male mouse RPE/choroids. A directionally cloned cDNA library in the pSPORT1 vector(Life Technologies) was constructed at Bioserve Biotechnology (Laurel MD) essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. cDNA was cloned in Not I/Sal I sites. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC).

text: Adult

characteristic: dev-stage

characteristic: lab-host

text: Eye

characteristic: organ

text: Mus musculus

text: Male

text: RPE/choroid

text: Mouse RPE/choroid, unamplified: mi/mj

text: Plasmid


text: National Eye Institute

text: live

text: Generic

text: 270ug total RNA was extracted from 200 adult male mouse retinas. A directionally cloned cDNA library in the pSPORT1 vector(Life Technologies) was constructed at Bioserve Biotechnology (Laurel MD) essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. cDNA was cloned in Not I/Sal I sites. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC).

text: Adult

text: Eye

text: Mus musculus

characteristic: organism

text: Male

characteristic: strain

text: retina

characteristic: tissue-type

text: Mouse retina, unamplified: mk/ml

text: Plasmid


characteristic: External Id

text: National Eye Institute

text: live

text: Generic

text: RNA was extracted from 75 adult male mouse lacrimal glands. A directionally cloned cDNA library in the pCMVSPORT6 vector(Life Technologies) was constructed at Bioserve Biotechnology (Laurel MD) essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC).

text: Adult

text: Eye

text: Mus musculus

text: Male

text: lacrimal gland

text: Mouse lacrimal gland, unamplified: ou

characteristic: title

text: Plasmid


text: National Eye Institute

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: Approximately 1mg total RNA was extracted from 200 adult mouse whole eyes. A directionally cloned cDNA library in the pSPORT1 vector (Invitrogen) was constructed at Bioserve Biotechnology (Laurel MD) essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. cDNA was cloned in Not I/Sal I sites. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC).

text: Adult

text: Eye

text: Mus musculus

text: Whole eye

text: Mouse Whole eye, unamplified: io/ip

text: Plasmid


text: Departament of Genetics, FMRP/USP, FUNDHERP

text: live

text: Generic

text: adult

text: whole body

text: Apis mellifera

text: female, worker

text: Africanized


text: live

text: Generic

text: embryonic

text: whole body

text: Apis mellifera

text: female, worker

text: Africanized


text: live

text: Generic

text: larval

text: whole body

text: Apis mellifera

text: female, worker

text: Africanized


text: live

text: Generic

text: Mix of adult, pupal, larval and embryonic.

text: whole body

text: Apis mellifera

text: female, worker

text: Africanized


text: live

text: Generic

text: pupal

text: whole body

text: Apis mellifera

text: female, worker

text: Africanized


text: live

text: Generic

text: The library was constructed according Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. Denatured RNA was size fractionated on a 1% agarose gel.First strand cDNA synthesis was primed with oligo-dT primer containing a Not I site. Double strand cDNA was size selected according to mRNA size fraction, ligated with EcoR I adaptor, digested with NotI and then cloned directionally into pYX-Asc vector. The library tag sequence located between the Not I site and the polyA tail is CGAACTGAAT. This library was created for the University Iowa Brain Anatomy Project (BMAP): 'Gene Discovery in the Developing Mouse Nervous System', supported by National Institute of Mental Health (NIMH).

text: 9.5-10.5 dpc

text: DH10B (T1 phage resistant)

text: Head

text: Mus musculus

text: EcoR I

text: Not I

text: Upper Head

text: Plasmid

text: pYX-Asc

characteristic: vector


text: Universite des Sciences et Technologies de Lille

characteristic: INSDC last update

text: live

text: Generic

text: Theromyzon tessulatum

text: hermaphrodite

text: entire animal

text: Leech differential Display RT-PCR products

text: Plasmid

text: pGEM-T Easy Vector System


text: Technische Universitaet Muenchen

text: live

characteristic: INSDC status

text: Generic

text: Bettina

characteristic: cultivar

text: cDNA library highly enriched for Phytophthora infestans induced genes derived from the moderately resistant potato cv. Bettina.

text: leaf

text: Solanum tuberosum

text: Bettina-lib


text: Technische Universitaet Muenchen

text: live

text: Generic

characteristic: NCBI submission model

text: Indira

text: cDNA library highly enriched for Phytophthora infestans induced genes derived from the susceptible potato cv. Indira.

characteristic: description

text: leaf

text: Solanum tuberosum

text: Indira-lib


text: IRD-CNRS-Universite de Perpignan-CIAT

text: live

text: Generic

characteristic: NCBI submission package

text: Stratagene lambda Zap Library

text: Root

text: Manihot esculenta

text: EcoRI

text: XhoI

text: CM523-7 cassava lambda zap

text: plasmid

text: pBluescript SK (+/-)


text: IRD-CNRS-Universite de Perpignan-CIAT

text: live

text: Generic

text: Stratagene lambda Zap Library

text: Root

text: Manihot esculenta

text: EcoRI

text: XhoI

text: MPer183 cassava lambda zap

text: plasmid

text: pBluescript SK (+/-)


text: IRD-CNRS-Universite de Perpignan-CIAT

text: live

text: Generic

text: Stratagene lambda Zap Library

text: Stem

text: Manihot esculenta

text: EcoRI

text: XhoI

text: MBra685 cassava lambda zap

text: plasmid

text: pBluescript SK (+/-)


text: IRD-CNRS-Universite de Perpignan-CIAT

text: live

text: Generic

text: Stratagene lambda Zap Library

text: Stem

text: Manihot esculenta

text: EcoRI

text: XhoI

text: MCol1522 cassava lambda zap

text: plasmid

text: pBluescript SK (+/-)


text: IRD-CNRS-Universite de Perpignan-CIAT

text: live

text: Generic

text: Library not subtracted. The cDNA was synthesized from RNA obtained from the inoculated tissues with Xanthomonas axonopodis pv manihotis strain Cio-151 and collected at 6, 12, 24, 48, 72 hpi and 5 and 7 dai using the protocol SSH (Clontech, Palo Alto, CA, USA)

text: Root, Stem

text: Manihot esculenta

text: EcoRI

text: EcoRI

text: MBra685 cassava not subtracted

text: plasmid

text: pGEMTeasy


text: IRD-CNRS-Universite de Perpignan-CIAT

text: live

text: Generic

text: Library subtracted using method of DSC (Luo et al., 1999); the cDNA synthesized from RNA obtained from the leaves healthy and wounded were pooled and used as 'drivers'. The cDNA from the inoculated tissues with Xanthomonas axonopodis pv manihotis strain Cio-151 was used as 'tester'. The tissue was collected at 6, 12, 24, 48, 72 hpi and 5 and 7 dai

text: Leaves

text: Manihot esculenta

text: EcoRI

text: EcoRI

text: MBra685 Cassava subtracted (DSC)

text: plasmid

text: pGEMTeasy


text: IRD-CNRS-Universite de Perpignan-CIAT

text: live

text: Generic

text: Library not subtracted. The cDNA was synthesized from RNA obtained from the inoculated tissues with Xanthomonas axonopodis pv manihotis strain Cio-151 and collected at 6, 12, 24, 48, 72 hpi and 5 and 7 dai using the protocol DSC (Luo et al., 1999).

text: Stem

text: Manihot esculenta

text: EcoRI

text: EcoRI

text: SG107-35 Cassava not subtracted (DSC)

text: plasmid

text: pGEMTeasy


text: IRD-CNRS-Universite de Perpignan-CIAT

text: live

text: Generic

text: Library subtracted using method SSH; the cDNA synthesized from RNA obtained from the stems healthy and wounded were pooled and used as 'drivers'. The cDNA from the inoculated tissues with Xanthomonas axonopodis pv manihotis strain Cio-151 was used as 'tester'. The tissue was collected at 6, 12, 24, 48, 72 hpi and 5 and 7 dai

text: Stem

text: Manihot esculenta

text: EcoRI

text: EcoRI

text: SG107-35 Cassava subtracted (SSH)

text: plasmid

text: pGEMTeasy


text: IRD-CNRS-Universite de Perpignan-CIAT

text: live

text: Generic

text: Library subtracted using method DSC (Luo et al., 1999); the cDNA synthesized from RNA obtained from the stems healthy and wounded were pooled and used as 'drivers'. The cDNA from the inoculated tissues with Xanthomonas axonopodis pv manihotis strain Cio-151 was used as 'tester'. The tissue was collected at 6, 12, 24, 48, 72 hpi and 5 and 7 dai

text: Stem

text: Manihot esculenta

text: EcoRI

text: EcoRI

text: SG107-35 Cassava subtracted (DSC)

text: plasmid

text: pGEMTeasy


text: IRD-CNRS-Universite de Perpignan-CIAT

text: live

text: Generic

text: Library subtracted using method SSH; the cDNA synthesized from RNA obtained from the stems healthy and wounded were pooled and used as 'drivers'. The cDNA from the inoculated tissues with Xanthomonas axonopodis pv manihotis strain Cio-46 was used as 'tester'. The tissue was collected at 6, 12, 24, 48, 72 hpi and 5 and 7 dai

text: Stem

text: Manihot esculenta

text: EcoRI

text: EcoRI

text: MCol1522 Cassava subtracted (SSH)

text: plasmid

text: pGEMTeasy


text: The Georgia Institute of Technology

text: live

text: Generic

text: 4th instar larva, pupa, and late adult

characteristic: dev-stage

text: Camponotus festinatus

text: Worker caste (female)

characteristic: sex

text: Light morph

text: Whole body

text: Camponotus festinatus composite library

text: Plasmid

text: Stratagene pBluescript II


text: The Natural History Museum

text: live

text: Generic

text: mRNA, extracted from schistosome-unexposed 3017 (resistant) snails was used as a template for RT-PCR with random primers to generate cDNA fragments. These were cloned and sequenced using M13F. Primer sequences were removed from sequence.

text: Ovotestis

characteristic: organ

text: Biomphalaria glabrata

text: Hermaphrodite

text: NHM 3017

text: BgORESTES unexposed NHM 3017 Ovotestis

text: pGEM


text: The Natural History Museum

text: live

text: Generic

text: mRNA, extracted from schistosome-exposed 3017 (resistant) snails was used as a template for RT-PCR with random primers to generate cDNA fragments. These were cloned and sequenced using M13F. Primer sequences were removed from sequence.

text: Ovotestis

text: Biomphalaria glabrata

characteristic: organism

text: Hermaphrodite

text: NHM 3017

text: BgORESTES schistosome-exposed NHM 3017 Ovotestis 2

text: pGEM


text: The Natural History Museum

text: live

text: Generic

text: Biomphalaria glabrata

text: Hermaphrodite

text: NHM 3017

characteristic: strain

text: Haemocytes

text: BgORESTES unexposed NHM 3017 Haemocytes

text: pGEM


text: The Natural History Museum

text: live

text: Generic

text: Biomphalaria glabrata

text: Hermaphrodite

text: NHM 3017

text: Haemocytes

characteristic: tissue-type

text: BgORESTES schistosome-exposed NHM 3017 Haemocytes

text: pGEM


characteristic: External Id

text: The Natural History Museum

text: live

text: Generic

text: Head/foot

text: Biomphalaria glabrata

text: Hermaphrodite

text: NHM 3017

text: BgORESTES unexposed NHM 3017 Head/foot

text: pGEM


text: The Natural History Museum

text: live

text: Generic

text: Biomphalaria glabrata

text: Hermaphrodite

text: NHM 3017

text: Head/foot

text: BgORESTES schistosome-exposed NHM 3017 Head/foot 2

characteristic: title

text: pGEM


text: Shinshu University School of Medicine

characteristic: INSDC center name

text: live

text: Generic

text: erythrocytic stage

text: Plasmodium berghei ANKA

text: ANKA

text: Plasmodium berghei strain ANKA erythrocytic stage


text: Qindu Dental School

characteristic: INSDC first public

text: live

text: Generic

text: osteoblast-like

characteristic: cell type

text: Homo sapiens

text: Homo sapiens osteoblast-like cells


text: National Museum of Marine biology and Aquarium

characteristic: INSDC last update

text: live

text: Generic

text: Symbiotic stage

text: Exaiptasia diaphana

text: Symbiotic sea anemone cDNA library


text: live

characteristic: INSDC status

text: Generic

text: Apoymbiotic stage

text: Exaiptasia diaphana

text: Aposymbiotic sea anemone cDNA library


text: live

text: Generic

text: Symbiotic and aposymbiotic stages

text: Exaiptasia diaphana

text: Sea anemone cDNA library


text: Genome Institute of Singapore

text: live

text: Generic

text: Priming method: Sfi-(dT)30 Primed ; Priming sequence: 5.ATTCTAGAGGCCGAGGCGGCCGACATG(T)30VN ; Directionally cloned, 5' cloning site: Sfi A site GGCCATTACGGCC ; 5' linker/adaptor sequence: 5.AAGCAGTGGTATCAACGCAGAGTGGCC ; 3' cloning site: Sfi B site GGCCGAGGCGGCC ; 3' linker/adaptor sequence: same as the priming sequence ; Average insert size: 2kb ; For PCR insert analysis: Use M13 Forward and reverse primers ; Library Amplified ; Recombinants (inserts): 98% ; Library complexity: 5x106 ; Full-length construction (method): SMART, a Clontech method. The pooled tissue RNA was collected and used to construct full length enriched cDNA library and also served as template to synthesize complex first strand cDNA probe. Two high density colony arrays were made from over 110K cDNA clones and hybridized with the probes. Clones with high hybridization signals were selected as they represented highly abundant expressed clones. The hybridization intensities for all clones span from 0 to 1.8 million counts and the high abundant class ranged from 210,000 to 1.8 million.

text: 7 Different embryonic Stages(From just fertilized Embryos to 72 hours just hatched baby fish)

characteristic: lab-host

text: Danio rerio

text: Sfi A (GGCCATTACGGCC)

text: Sfi B (GGCCGAGGCGGCC)

text: Singapore local strain

text: Embryo

text: Plasmid

text: pDNR-LIB

characteristic: vector


text: Genome Institute of Singapore

text: live

text: Generic

characteristic: NCBI submission model

text: Priming method: Sfi-(dT)30 Primed ; Priming sequence: 5.ATTCTAGAGGCCGAGGCGGCCGACATG(T)30VN ; Directionally cloned, 5' cloning site: Sfi A site GGCCATTACGGCC ; 5' linker/adaptor sequence: 5.AAGCAGTGGTATCAACGCAGAGTGGCC ; 3' cloning site: Sfi B site GGCCGAGGCGGCC ; 3' linker/adaptor sequence: same as the priming sequence ; Average insert size: 2kb ; For PCR insert analysis: Use M13 Forward and reverse primers ; Library Amplified ; Recombinants (inserts): 98% ; Library complexity: 5x106 ; Full-length construction (method): SMART, a Clontech method The pooled tissue RNA was collected and used to construct full length enriched cDNA library and also served as template to synthesize complex first strand cDNA probe. Two high density colony arrays were made from over 110K cDNA clones and hybridized with the probes. Low intensity clones were selected as they represented rare expressed clones. The hybridization intensities for all clones span from 0 to 1.8 million counts and the low abundant class ranged from 0 to 13,000.

characteristic: description

text: Danio rerio

text: Sfi A (GGCCATTACGGCC)

text: Sfi B (GGCCGAGGCGGCC)

text: Singapore local strain

text: Embryo

text: Plasmid

text: pDNR-LIB


text: College of Life Sciences, ShenZhen University

text: live

text: Generic

characteristic: NCBI submission package

text: E.coli NM522

text: Artemisia apiacea

text: Artemisia apiacea Hance cDNA library

text: Phage

text: lambda Excell


text: General Hospital of PLA

text: live

text: Generic

text: lymphocyte

text: SSH library of recovered SARS patients as tester and healthy patients as driver

text: Homo sapiens

text: blood

text: SARS patients lymphocyte SSH


text: Genome Institute of Singapore

text: live

text: Generic

text: Priming method: Sfi-(dT)30 Primed ; Priming sequence: 5.ATTCTAGAGGCCGAGGCGGCCGACATG(T)30VN ; Directionally cloned, 5' cloning site: Sfi A site GGCCATTACGGCC ; 5' linker/adaptor sequence: 5.AAGCAGTGGTATCAACGCAGAGTGGCC ; 3' cloning site: Sfi B site GGCCGAGGCGGCC ; 3' linker/adaptor sequence: same as the priming sequence ; Average insert size: 2kb ; For PCR insert analysis: Use M13 Forward and reverse primers ; Library Amplified ; Recombinants (inserts): 98% ; Library complexity: 5x106 ; Full-length construction (method): SMART, a Clontech method ; The pooled tissue RNA was collected and used to construct full length enriched cDNA library and also served as template to synthesize complex first strand cDNA probe. Two high density colony arrays were made from over 110K cDNA clones and hybridized with the probes. Medium intensity clones were selected as they represented modest abundant expressed clones. The hybridization intensities for all clones span from 0 to 1.8 million counts and the medium abundant class ranged from 25,000 to 50,000.

characteristic: dev-stage

text: Danio rerio

text: Sfi A (GGCCATTACGGCC)

text: Sfi B (GGCCGAGGCGGCC)

text: Singapore local strain

text: Embryo

text: Plasmid

text: pDNR-LIB


text: Centers for Disease Control and Prevention

text: live

text: Generic

text: Genes differentially expressed in PBMCs of a CFS patient were identified by differential display PCR.

text: Homo sapiens

text: Human peripheral blood mononuclear cells (PBMCs)


text: The Canadian Potato Genome Project - BioAtlantech

text: live

text: Generic

text: Shepody

characteristic: cultivar

text: supplier: Developmental series. Plants from pathogen-free Solanum tuberosum var. Shepody, clone 1756, nuclear stock were grown in a screenhouse under natural conditions. RNA was isolated from swollen stolon tissue, 3-10mm in length, which was cut from the tip, to the base of swelling.

text: Solanum tuberosum

text: EcoRI

text: XhoI

text: Stolon

text: Swollen Stolon

text: pBluescript II SK(+) XR


text: University of Nijmegen

text: live

text: Generic

text: Petit Havanna SR1

text: Anther

characteristic: organ

text: Nicotiana tabacum

characteristic: organism

text: Tobacco anther cDNA


text: University of Nijmegen

text: live

text: Generic

text: Petit Havanna SR1

text: Stigma

text: Nicotiana tabacum

text: Tobacco stigma cDNA


text: University of Nijmegen

text: live

text: Generic

text: Petit Havanna SR1

text: young roots

text: Root

text: Nicotiana tabacum

text: Tobacco root cDNA


text: Instituto de Genetica e Bioquimica, Universidade Federal de Uberlandia

text: live

text: Generic

text: cDNA fragment cloned from Larva 1/Larva 2 differentially gene-expressed profiles during Melipona scutellaris post-embryonic development

text: Melipona scutellaris

text: EcoRI

text: EcoRI

text: larvae, pupae and adult (queens - workers)

characteristic: sex

text: Melipona scutellaris DDRT-PCR

text: pCR 2.1


characteristic: External Id

text: Genoscope - Centre National de Sequencage

text: live

text: Generic

text: Arabidopsis thaliana

characteristic: strain

text: Adult vegetative tissue

characteristic: tissue-type

text: Arabidopsis thaliana Adult vegetative tissue Col-0


characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: Arabidopsis thaliana

text: Hormone Treated Callus

text: Arabidopsis thaliana Hormone Treated Callus Col-0

characteristic: title


characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Arabidopsis thaliana

text: Silique

text: Arabidopsis thaliana Silique Col-0


text: live

text: Generic

text: Arabidopsis thaliana

text: Flowers and buds

text: Arabidopsis thaliana Flowers and buds Col-0


text: AG Kraemer, MPI of Molecular Plant Physiology

text: live

text: Generic

text: ecotype: Langelsheim

text: Arabidopsis halleri subsp. halleri

text: root

text: Arabidopsis halleri subsp. halleri root


text: Zhejiang University

text: live

text: Generic

text: The appressorium cDNA library was constructed from appressorium formed for 23.5-24.5 hr on an inductive surface at 25oC. The library contains over 2.37x106 independent clones.

text: 24hr appressorium on appressorium-inductive surface

text: Pyricularia grisea

text: Sfi I

text: Sfi I

text: Magnaporthe grisea strain Guy11 Appressorium Stage cDNA Library

text: Phage

text: lambda TriplEx2

characteristic: vector


text: University of Tokyo

text: live

text: Generic

characteristic: NCBI submission model

text: adult

text: Apis mellifera

text: brain

text: Apis mellifera brain adult


text: College of Life Science

text: live

text: Generic

text: Hepatocellular carcinoma cells

characteristic: cell type

characteristic: cell-line

text: Differentially expressed genes in hepatocellular carcinoma cells SMMC-7721 response to Typhonium giganteum Engl., then the genes were cloned in DH5a, the vector is PBS-T

characteristic: description

text: Response to typhonium giganteum Engl.

text: Cells

characteristic: isolate

characteristic: lab-host

text: Liver

text: Homo sapiens

text: Male

text: Liver cancer

text: Hepatocellular carcinoma cells DDRT-PCR Lib

text: PBS-T


text: Centre de Recherche du CHUL, Universite Laval

text: live

text: Generic

characteristic: NCBI submission package

text: Muller cell

text: Subtraction of Muller cells cDNA (tester) was performed with the PCR-Select cDNA subtraction kit (Clontech). Driver cDNA consisted of cerebral astrocytes. Tester and driver cDNAs were digested by RsaI; adaptors were ligated to the tester. Two rounds of subtractive hybridization were performed, followed by two suppression PCR reactions. Subtracted PCR products were ligated into a T/A vector (Promega)

text: DH5 alpha

text: Eye

characteristic: organ

text: Homo sapiens

characteristic: organism

text: Pooled

text: Retina

text: Human Muller cells library

text: Plasmid

text: PGEM-T (Promega)


characteristic: External Id

text: Third Military Medical University of ChongQing, China

text: live

text: Generic

text: liver cancer

text: liver cancer cDNA Lib obtained by Suppression subtractive hybridization EST obtained by RACE (rapid amplification of cDNA ends)

text: subculturing 20 Generation

characteristic: dev-stage

text: Homo sapiens

text: EcoRV T-Cloning site

text: Human liver cancer cDNA Lib

text: T-A cloning vector(plasmid)

text: (PMD18-T vector)


text: Washington University School of Medicine

characteristic: INSDC center name

text: live

text: Generic

text: The cDNA library was constructed by Keliang Tang, adn Robert Cole at Washington University. Total RNA was converted to cDNA using the template-switching PCR method (Creator SMART cDNA, Clontech, Inc.). First strand was reverse transcribed using the CDS III/3' primer and a 5' template switch primer using the same primer set and the fragments were digested with SfiI. The directional SfiI sites, and electroporated into GC10 Competent cells. Antibiotic: Chloramphenicol (30ug/ml)

text: Tachyzoite

text: Toxoplasma gondii

text: SfiI

text: SfiI

text: TgRH Tachyzoite FL cDNA

text: pDNR-LIB


text: Ottawa Health Research Institute

characteristic: INSDC first public

text: live

text: Generic

text: Subtracted cDNA library from regenerating caudal fin at 4 day post-amutation (4dpa). Two micrograms of mRNA from intact and 4dpa fins were used for cDNA synthesis. First strand and second strand cDNAs were synthesized using the enzymes and reagents supplied in the PCR-select subtraction kit (Clontech Laboratories, Inc, Palo Alto, CA, USA). Subtraction was performed using cDNA from 4 dpa fins as tester and intact fins as driver. To evaluate the efficiency of the cDNA subtraction, we determined, in a semi-quantitative PCR procedure, the amounts of cDNA for a house-keeping gene, beta-actin, presumably not affected by regeneration, in subtracted and non-subtracted cDNA pools. Detection of beta-actin required 20 cycles of PCR in the case of 4 dpa and 1 dpa subtracted cDNAs, whereas only 15 cycles were sufficient to amplify beta-actin from non-subtracted cDNA pools. This five-cycle difference in amplification roughly corresponds to more than 30-fold cDNA enrichment in the subtracted DNA suggesting that the amount of housekeeping transcripts was efficiently reduced. The subtracted cDNAs were cloned in pCR II vector using the T/A cloning kit (both from Invitrogen Life Technologies) and the resulting libraries were propagated in the host DH5-alpha bacteria. The library was constructed by Bhaja K. Padhi and Lucille Joly.

text: 4 day regeneration

text: E. coli DH5 alpha

text: Caudal fin

text: Danio rerio

text: EcoRI

text: Mixed

characteristic: sex

text: Wild-type

characteristic: strain

text: Danio rerio four-day regenerating caudal fin subtracted cDNA

text: Plasmid

text: pCR II


text: Ottawa Health Research Institute

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Subtracted cDNA library from regenerating caudal fin at 1 day post-amutation (1dpa). Two micrograms of mRNA from intact and 1dpa fins were used for cDNA synthesis. First strand and second strand cDNAs were synthesized using the enzymes and reagents supplied in the PCR-select subtraction kit (Clontech Laboratories, Inc, Palo Alto, CA, USA). Subtraction was performed using cDNA from 1 dpa fins as tester and intact fins as driver. To evaluate the efficiency of the cDNA subtraction, we determined, in a semi-quantitative PCR procedure, the amounts of cDNA for a house-keeping gene, beta-actin, presumably not affected by regeneration, in subtracted and non-subtracted cDNA pools. Detection of beta-actin required 20 cycles of PCR in the case of 4 dpa and 1 dpa subtracted cDNAs, whereas only 15 cycles were sufficient to amplify beta-actin from non-subtracted cDNA pools. This five-cycle difference in amplification roughly corresponds to more than 30-fold cDNA enrichment in the subtracted DNA suggesting that the amount of housekeeping transcripts was efficiently reduced. The subtracted cDNAs were cloned in pCR II vector using the T/A cloning kit (both from Invitrogen Life Technologies) and the resulting libraries were propagated in the host DH5-alpha bacteria. The library was constructed by Bhaja K. Padhi and Lucille Joly.

text: 1 day regeneration

text: E. coli DH5 alpha

text: Caudal fin

text: Danio rerio

text: EcoRI

text: Mixed

text: Wild-type

text: Danio rerio one-day regenerating caudal fin subtracted cDNA

characteristic: title

text: Plasmid

text: pCR II


text: Southwest Agricultural University

text: live

text: Generic

characteristic: NCBI submission model

text: Young fruit of 6, 12, 24, 48, 72 hours after pollination and of 0, 3 days without pollination after flowering

text: Young fruit before and after pollination within 3 days

text: Fruit

text: Cucumis sativus

text: Female (gynoecious)

text: Cucumber cDNA-AFLP cDNA fragments

text: pUCm-T

characteristic: vector


text: live

text: Generic

text: Rattus norvegicus

text: testicle

characteristic: tissue-type

text: Rat testicle cDNA library


text: Penn State University

text: live

text: Generic

text: This is a directionally cloned, non-normalized library. This library has been generated by the Floral Genome Project (FGP). The Floral Genome Project is funded by NSF's Plant Genome Research Program (DBI-0115684). More information about the project can be obtained at http://fgp.bio.psu.edu

characteristic: description

text: 4mm buds

text: SOLR

text: Amborella trichopoda

text: EcoRI

text: female flower buds

text: Phagemid

text: pBluescript SK (+/-)


text: Penn State University

text: live

text: Generic

text: <1mm buds

text: SOLR

characteristic: lab-host

text: Cucumis sativus

text: EcoRI

text: male flower buds

text: Phagemid

text: pBluescript SK (+/-)


text: Penn State University

text: live

text: Generic

characteristic: NCBI submission package

text: <1mm buds

text: SOLR

text: Cucumis sativus

text: EcoRI

text: female flower buds

text: Phagemid

text: pBluescript SK (+/-)


text: Penn State University

text: live

text: Generic

text: 1-35 mm buds

text: SOLR

text: Liriodendron tulipifera

text: EcoRI

text: flower buds

text: Phagemid

text: pBluescript SK (+/-)


text: Penn State University

text: live

text: Generic

text: 12-20 mm buds

text: SOLR

text: Persea americana

text: EcoRI

text: flower buds

text: Phagemid

text: pBluescript SK (+/-)


text: Penn State University

text: live

text: Generic

text: SOLR

text: Welwitschia mirabilis

text: EcoRI

text: young male strobilus

text: Phagemid

text: pBluescript SK (+/-)


text: Penn State University

text: live

text: Generic

text: SOLR

text: Welwitschia mirabilis

text: EcoRI

text: young female strobilus

text: Phagemid

text: pBluescript SK (+/-)


text: TNO Nutrition and Food Research Institute

text: live

text: Generic

text: Aspergillus niger

text: Aspergillus niger smF cDNA Library


text: University of Parma

text: live

text: Generic

text: Aegilops umbellulata

text: Ae. umb. 447

text: 2 weeks plantlets

text: Aegilops umbellulata 2 weeks plantlets Ae. umb. 447


text: University of Missouri-Columbia

text: live

text: Generic

text: Funding: The production of ESTs submitted in this project was funded by USDA Grant MRI-2002-03476 entitled 'Bovine ESTs: Focus on Female Reproduction' to RS Prather, E Antoniou, HA Garverick, JA Green, MC Lucy, RM Roberts, MF Smith and RS Youngquist. Genetic Source: Heifers for the project were purchased from Circle A Ranch, Iberia, MO (http://www.circlearanch.com/home.html). These heifers, while not registered have known Angus pedigrees going back at least 4 generations. Samples collected: The samples consisted of the following: germinal vesicle-stage oocytes; in vitro derived embryos (2-cell, morula, blastocyst and nuclear transfer blastocyst); in vivo blastocysts and conceptuses (days 8, 14, 16 and 18); corpora lutea (days 3, 5, 8, 14, 16, 18 and 35); ovarian follicles (days 0, non-recruited, recruited, early selected and preovulatory); oviduct (days 0, 3 and 5); endometrium (days 5, 8, 14, 16, 18 and 35); and placenta/embryo from day 35 conceptuses. Expanded descriptions of how the tissues were collected can be found at the following URL: http://genome.rnet.missouri.edu/Bovine/Methods.html. Library construction (Standard Protocol): All procedures have been described in detail elsewhere (Soares et al., 1994; Bonaldo et al., 1996; Jiang et al., 2001). Total cellular RNA from each sample was isolated by using STAT-60 reagent (Tel-Test, Friendswood, TX) and the poly(A)+ RNA was obtained by two rounds of purification with the Oligotex mRNA isolation kit (Qiagen) according to the manufacturer''s instructions. The libraries were constructed by E. Ferguson and R. Woods essentially as described by the manufacturer's instructions provided with the SuperScript Plasmid System (Invitrogen, cat. no. 18248-013). Briefly, 1mcg of poly(A)+ RNA was annealed at c37 degrees with 10mcg of NotI-tag-dT18 oligonucleotide (GCTGCTCGCGGCCGC-tag-T18) and reverse transcribed at c37 degrees with SuperScript II (Invitrogen) reverse transcriptase (Jiang et al., 2001). The 'tag' represents a tissue/stage-specific ten-base sequence identifier (http://genome.uiowa.edu/pubsoft/software.html) present in the oligonucleotide used to prime first-strand synthesis. Second strand synthesis was performed with T4 DNA polymerase in the presence of DNA ligase and RNase H. After second strand synthesis, the double-stranded cDNAs were ligated to SalI adapters (Invitrogen-Life Technologies) and digested with NotI. The cDNAs were size selected by passage through cDNA size fractionation columns (Invitrogen-Life technologies). The cDNAs derived from each developmental stage of a particular tissue were mixed on an equimolar basis and ligated directionally into the NotI and SalI sites of the pCMV-SPORT6 vector (Invitrogen). After ligation of the inserts, the plasmids were electroporated into DH10B bacteria. Library Construction (PCR Protocol): The amount of mRNA that was recovered from oocytes and embryos was quite limiting and was not sufficient for library production with the standard protocol. Therefore, PCR-based protocol was utilized for producing libraries from sources in which the amount of extracted mRNA was small (oocytes and embryos). Poly-A RNA was isolated by using the MicroPoly(A) Pure kit from Ambion (cat. # 1918). The mRNA was reverse transcribed with a NotI-tag-dT18 oligonucleotide and a SMART oligonucleotide (Clontech) modified to contain a SalI site to generate full-length cDNA with a sequence complementary to the SMART oligonucleotide. Sequences within the SMART and dT oligonucleotides were used as primers to amplify the cDNAs by PCR with pfu turbo polymerase (Stratagene). The resulting PCR products were purified, digested with NotI and SalI and size fractionated by using Chroma Spin-1000 columns (Clontech). Purified cDNA from each PCR reaction was quantitated and mixed on an equimolar basis for ligation into the pCMV- SPORT6 vector. Preliminary Library Characterization: Randomly chosen clones from each library were analyzed by restriction digestion to determine average insert size (96 clones) and by sequencing (~4 96-well plates) to confirm library quality [e.g. the presence of short polyA+ tails, genomic DNA contamination (must be <1%), ribosomal RNA clones (must be <1%), etc.] and to provide a sequence database representing the predominant clones in each library. The clones were sequenced at the University of Missouri-Columbia DNA Core Facility. After production of the libraries, equal numbers of recombinants from each library were pooled to produce a single mixed library (mega-library) for more extensive sequencing. Bioinformatics work was performed by GK Springer's bioinformatics group (Spollen WG, Topinka CM, Khambati AA) in Computer Science at the University of Missouri-Columbia. Clone Requests: Requests for clones should be made to the Director of the University of Missouri DNA Core facility at: bovine@rnet.missouri.edu. Bonaldo MF, Lennon G, Soares MB,. Normalization and Subtraction: Two approaches to facilitate gene discovery. Genome Res, 1996; 6:791-806. Jiang H, Bivens NJ, Ries JE, Whitworth KM, Green JA, Forrester LJ, Springer GK, Didion BA, Mathialagan N, Prather RS, Lucy MC (2001) Constructing cDNA libraries with fewer clones that contain long poly(dA) tails. Biotechniques 31:38-42. Soares MB, MF Banaldo, P Jelene, L Su, L Lawton, A Efstrantiadis. 1994. Construction and characterization of a normalized cDNA library. Proc Natl Acad Sci, 91:9228-9232.

text: Bos taurus

text: bemiv


text: University of Missouri-Columbia

text: live

text: Generic

text: Bos taurus

text: bend


text: University of Missouri-Columbia

text: live

text: Generic

text: Bos taurus

text: bov


text: USDA-ARS and University of Minnesota

text: live

text: Generic

text: Ogle

characteristic: cultivar

text: Oat cDNA library prescreened against seed storage proteins, globins, and avenins. Cloned into lambda unizap-XR cosmid vectors and converted into pBluescript plasmid. Note: UMN clones designated less than 5000 are from this cDNA library. Others are from a different oat genomic library. Library was made at University of Minnesota. Ref: O'Donoughue et al (1995) Genome 38: 368-380.

text: immature endosperm

text: Avena sativa

text: EcoRI

text: XhoI

text: endosperm

text: UMN cDNA from oat

text: Plasmid

text: Bluescript SK-


text: National Cancer Institute / NIH

text: live

text: Generic

text: Cells were isolated by flow cytometry and RNA isolated in Trizol reagent. cDNA was obtained by reverse transcription using oligo-dT primer 5'-GACCACGCGTATCGATGTCGACTTTTTTTTTTTTTTTTV-3'and treated with ExoI. PCR was performed using a complementary 3' primer containing a BstXI site and a cap-switch 5' primer. The resulting cDNA was digested with BstXI and ligated to a BstXI adaptor 5'-AAAAAAAAAAAAAAAAGTCGACATCGATACGCGTGGTCTCGTAGCTGACTTTCCAGCACA-3' then digested with AscI. Size-selection was performed >0.5 kb to result in an average insert size of 0.8 kb. Library constructed and contributed by Mike Clarke and Andrew Hass (University of Michigan). Note: this is an MGC library.

text: breast

characteristic: organ

text: Homo sapiens

characteristic: organism

text: AscI

text: BstXI

text: cancer cells growing in a xenograft mouse model

text: phagemid


characteristic: External Id

text: National Cancer Institute / NIH

text: live

text: Generic

text: RNA obtained from 6 adult male testis. cDNA was primed using oligo-dT primer: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3' and cloned into the EcoRV/NotI sites of pExpress-1. Size-selection >1kb resulted in an average insert size of 1.15 kb. This primary, microquantity library is normalized to Cot5 (non-normalized primary library is NICHD_XGC_Te2) and was constructed by Express Genomics (Frederick, MD). Note: this is an NIH_XGC library

text: DH10B TonA

text: testis

text: Xenopus laevis

text: EcoRV

text: NotI

text: Pooled samples from 6 adult male testis

text: plasmid


text: National Cancer Institute / NIH

characteristic: INSDC center name

text: live

text: Generic

text: RNA obtained from 5 normal wild-type mice. cDNA was primed using oligo-dT primer: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3' and cloned into the EcoRV/NotI sites of pExpress-1. Size-selection 1.4 kb resulted in an average insert size of 1.2 kb. This primary, nanoquantity library is normalized to Cot5 (non-normalized primary library is NIH_MGC_230) and was constructed by Express Genomics (Frederick, MD). Note: this is a NIH_MGC library

text: DH10B TonA

text: thyroid

text: Mus musculus

text: NotI

text: NotI

text: Pooled thyroids from 5 mice

text: plasmid


text: National Cancer Institute / NIH

characteristic: INSDC first public

text: live

text: Generic

text: RNA obtained from 5 normal wild-type mice thyroid. cDNA was primed using oligo-dT primer: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3' and cloned into the EcoRV/NotI sites of pExpress-1. Size-selection 1.4 kb resulted in an average insert size of 1.2 kb. Normalized version of this library is NIH_MGC_189ibrary constructed by Express Genomics (Frederick, MD). Note: this is a NIH_MGC Library.

text: DH10B TonA

text: thyroid

text: Mus musculus

text: NotI

text: NotI

text: Pooled thyroids from 5 mice

text: plasmid


text: University of Parma

text: live

text: Generic

text: 2 week plantlets

characteristic: dev-stage

text: Aegilops umbellulata

text: Aegilops umbellulata 447 2 week plantlets


text: Graduate School of Bioagricultural Sciences, Nagoya University

text: live

text: Generic

text: adult worker

text: Apis mellifera

text: brain

text: Apis mellifera brain adult worker


text: National Cancer Institute / NIH

text: live

text: Generic

text: RNA obtained from 6 adult male testis. cDNA was primed using oligo-dT primer: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3' and cloned into the EcoRV/NotI sites of pExpress-1. Size-selection >1kb resulted in an average insert size of 1.25 kb. This is a primary library (normalized primary library is NICHD_XGC_Te2N) and was constructed by Express Genomics (Frederick, MD). Note: this is an NIH_XGC library

text: DH10B TonA

text: testis

text: Xenopus laevis

text: EcoRV

text: NotI

text: Pooled samples from 6 adult Xenopus testis

text: plasmid


text: live

text: Generic

text: Rattus norvegicus

text: Sprague-Dawley

characteristic: strain

text: rat,Sprague-Dawley


text: National Cancer Institute / NIH

characteristic: INSDC last update

text: live

text: Generic

text: Cells were isolated by flow cytometry and RNA isolated in Trizol reagent. cDNA was obtained by reverse transcription using oligo-dT primer 5'-GACCACGCGTATCGATGTCGACTTTTTTTTTTTTTTTTV-3'and treated with ExoI. PCR was performed using a complementary 3' primer containing a BstXI site and a cap-switch 5' primer. The resulting cDNA was digested with BstXI and ligated to a BstXI adaptor 5'-AAAAAAAAAAAAAAAAGTCGACATCGATACGCGTGGTCTCGTAGCTGACTTTCCAGCACA-3' then digested with AscI. Size-selection was performed >0.5 kb to result in an average insert size of 0.7 kb. Library constructed and contributed by Mike Clarke and Andrew Hass (University of Michigan). Note: this is an MGC library.

text: stem cell

text: Mus musculus

text: AscI

text: BstXI

text: multipotent committed hematopoietic progenitor cells

text: phagemid


text: MIPS

text: live

characteristic: INSDC status

text: Generic

text: adult

text: Homo sapiens

text: NotI

text: SalI

text: lymph node

text: 667 (synonym: hlno2)


text: National Institute of Genetics, Center for Information Biology and DNA Data Bank of Japan

text: live

text: Generic

text: adult

text: Lethenteron camtschaticum

text: brain

text: Lethenteron camtschaticum lambda ZAPII

characteristic: title

text: lambda ZAPII


text: Portland State University

text: live

text: Generic

characteristic: NCBI submission model

text: Liver

text: Austrofundulus limnaeus

text: Male

characteristic: sex

text: Quisiro

text: Killifish Temperature Acclimation Library


text: Zhejiang University

text: live

text: Generic

text: The appressorium SSH cDNA library was constructed from appressorium cDNA (formed for 23.5-24 .5 hr on an inductive surface) subtracted by cDNA of conidia, aerial mycelium and substrate mycelium in PCR-Select cDNA subtractive hybridization method.

text: 24hr appressorium on appressorium-inductive surface

text: Pyricularia grisea

text: Magnaporthe grisea Appressorium Stage SSH cDNA Library

text: pBlueScript II SK(+) Vector

characteristic: vector


text: George Washington University

text: live

text: Generic

text: coelomocyte

characteristic: cell type

text: directionally cloned

characteristic: description

text: Strongylocentrotus purpuratus

text: EcoRI

text: XhoI

text: Bacterially activated coelomocyte, arrayed

text: pBK-CMV