text: University Federal of Vicosa

text: live

text: Generic

text: Hibrido de Timor 832/2

characteristic: cultivar

text: From coffee plant inoculated with Hemileia vastatrix. Genotype: resistant to coffee leaf rust.

text: leaf

text: Coffea arabica x Coffea canephora

text: leaf

text: Coffea arabica x Coffea canephora cDNA-AFLP fragments


status: PUBLIC

characteristic: External Id

text: National Institute of Allergy and Infectious Diseases

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Unidirectional library was constructed from female ticks attached to dogs for 5 days using long distance PCR (Smart cDNA kit) and Lambda TriplEx2 vector.

characteristic: description

text: adult female

tag: attribute

characteristic: dev-stage

text: salivary glands

characteristic: organ

text: Rhipicephalus sanguineus

characteristic: organism

text: female

characteristic: sex

text: Adult female salivary gland cDNA library RsSGL2

characteristic: title

text: Phage

text: Lambda TriplEx2

characteristic: vector


text: live

text: Generic

text: Unidirectional library was constructed from female ticks attached to dogs for 3, 4 and 5 days using long distance PCR (Smart cDNA kit) and Lambda TriplEx2 vector.

text: adult female

text: salivary glands

text: Rhipicephalus sanguineus

text: female

text: Adult female salivary gland cDNA library RsSGL1

text: Phage

text: Lambda TriplEx2


text: University of Alberta

text: live

text: Generic

text: polyA RNA, Superscript Plasmid System (Invitrogen)

text: 14 day old seedlings

text: Carthamus tinctorius

text: whole plant

characteristic: tissue-type

text: Safflower

text: plasmid

text: pCMV.SPORT 6


text: Central Salt and Marine Chemicals Research Institute (CSIR)

text: live

text: Generic

text: Library prepared by subtracting cDNA expressed under 5.5M salt with cDNA expressed under 3M salt

text: Dunaliella salina

text: Dunaliella salina differentially expressed cDNA library


text: University of Kentucky

text: live

text: Generic

text: Prepared using CloneTech SMART cDNA cloning system: Zhu YY, Machleder EM, Chenchik A, Li R, and Siebert PD. 2001. Reverse transcriptase template switching: A SMART (TM) approach for full-length cDNA library construction. Biotechniques 30: 892-897.

text: Root

text: Lolium arundinaceum

text: SfiI

text: SfiI

text: hermaphrodite

text: Phagemid

text: lambda TriplEX2


text: Wellcome Trust Sanger Institute

text: live

text: Generic

text: Glossina morsitans morsitans

text: antennae

text: Glossina morsitans morsitans antennae


text: INRA

text: live

text: Generic

text: fruit body

text: One Shot TOP10

characteristic: lab-host

text: Agaricus bisporus

text: Bs738 (JB137)

characteristic: strain

text: Agaricus bisporus strain Bs738 (JB137) cDNA-AFLP library


characteristic: External Id

text: South Australian Research and Development Institute

text: live

text: Generic

text: Subtracted libraries from boron tolerant and intolerant bulked doubled hploid barley lines treated with 200 micro M boron. Root samples were collected from 2 weeks old hydroponically grown barley seedlings 24 hours after initiation of boron treatment. Suppression subtractive hybridizations were carried out using cDNA populations from tolerant and intolerant barley lines.

text: Roots

text: Hordeum vulgare subsp. vulgare

text: Boron tolerant and intolerant bulked doubled hploid barley lines treated with 200 micro M boron


characteristic: INSDC center name

text: live

text: Generic

text: Subtracted libraries from boron tolerant and intolerant bulked doubled hploid barley lines. Root samples were collected from 2 weeks old hydroponically grown barley seedlings. Suppression subtractive hybridizations were carried out using cDNA populations from tolerant and intolerant barley lines.

text: Roots

text: Hordeum vulgare subsp. vulgare

text: Boron tolerant and intolerant bulked doubled hploid barley lines


text: Beijing Academy of Agriculture and Forestry Science

characteristic: INSDC first public

text: live

text: Generic

text: The seedlings with two true leaves were inoculated with a spore suspension of Peronospora parasitica, and the second true leaves were harvested at 6h, 12h and 24h post-inoculation for total RNA extraction. For constructing the SSH-cDNA library, the mixed total RNA samples (eaqual amounts of samples from the three time points) digested with DNase were used as the 'tester', and the control from mock inoculation with water as the 'driver'.

text: Brassica rapa

text: hermaphrodite

text: leaves

text: Brassica rapa Peronospora parasitica-induced cDNA library by Suppression Subtractive Hybridization


characteristic: INSDC last update

text: live

text: Generic

text: microspore

characteristic: cell type

text: 33oC heat stressed for 24h

text: Brassica rapa

text: hermaphrodite

text: flower

text: Brassica rapa microspore embryogenesis related-gene


text: Yangzhou University

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Powdery mildew resistance near-isogenic line

characteristic: cultivar

text: Third leaf stage

text: Cucumis sativus

text: leaf

text: Suppression subtractive cDNA library of cucumber near-isogenic lines with high powdery mildew resistance


text: United Planters' Association of Southern India-Tea Research Foundation, Tea Research Institute

text: live

text: Generic

characteristic: NCBI submission package

text: PCR-select cDNA subtraction library (Clonetech)

characteristic: description

text: Camellia sinensis

text: Apical bud

text: Forward subtractive library from dormant and active buds of Camellia sinensis

text: Plasmid


text: live

text: Generic

text: Camellia sinensis

characteristic: organism

text: Apical bud

text: Reverse subtractive library from dormant and active buds of Camellia sinensis

text: Plasmid


text: Max Planck Institute for Chemical Ecology

text: live

text: Generic

text: normalized cDNA library construction using the Trimmer-Direct cDNA normalization kit (www.evrogen.com) and SMART technology (www.clontech.com)

text: first instar

characteristic: dev-stage

text: Heliothis virescens

text: SfiI

text: SfiI

text: midgut

text: cDNAs of Heliothis virescens regulated during interaction with its native host Nicotiana attenuata

text: plasmid

text: pUCLIB


text: International Center of Tropical Agriculture (CIAT)

text: live

text: Generic

text: basilisk

text: This is a subtractive library constructed by Andres Salcedo and enriched in genes induced by Aluminium stoxicity in roots of the resistant U. decumbens cv basilsk.

text: Root

characteristic: organ

text: Urochloa decumbens

text: Urochloa decumbens Alu

characteristic: title

text: Plasmid

text: pGEM-T Easy


text: live

text: Generic

text: CIAT-36062 interspecific hybrid

text: This is a subtractive library constructed by Catalina Romero and enriched in genes induced by spittlebug attack in roots of the resistant genotype CIAT-36062.

text: Root

text: Brachiaria sp. CIAT-36062

text: Brachiaria DSC

text: Plasmid

text: pGEM-T Easy

characteristic: vector


text: live

text: Generic

text: Mix of 40 different Brachiaria genotypes

text: This library was constructed by Federico Roda and contains transcripts expressed in roots of several Brachiaria lines submitted to variate conditions

text: Root

text: Brachiaria mixed EST library

text: Brachiaria SS

text: Plasmid

text: pGEM-T Easy


text: live

text: Generic

text: This is a subtractive library constructed by Federico Roda and containing transcripts (1) more expressed in resistant lines that in susceptible ones and (2) induced by spittlebug attack in these lines.

text: Root

text: Brachiaria mixed EST library

text: Brachiaria SUS

text: Plasmid

text: pGEM-T Easy


text: Imperial College London

text: live

text: Generic

text: The epidermis of infected barley collected 7 days after inoculation with Blumeria graminis f sp hordei DH14. The epiphytic fungal structures are removed prior to dissection of the epidermis. This sample contains barley epidermal cells (including stomata) many of which are colonised by one or more (up to 5 or 6) haustoria.

text: Supercharge Easy 10 electrocompetent E. coli

characteristic: lab-host

text: Blumeria hordei DH14

text: SfilA

text: SfilB

text: DH14 Epidermis

text: plasmid, Chloramphenicol resistant

text: pDNR-LIB


text: Imperial College London

text: live

text: Generic

text: Epiphytic Blumeria graminis f sp hordei DH14 collected 5 days after inoculation onto barley leaves. At this stage the epiphytic mycelium is fully developed, and active conidiophores are produced from which long chains of airborne conidia are formed.

text: Blumeria hordei DH14

text: SfilA

text: SfilB

text: DH14 5dpi

text: plasmid, Chloramphenicol resistant

text: pDNR-LIB


text: Imperial College London

text: live

text: Generic

text: Epiphytic Blumeria graminis f sp hordei DH14 collected 20 hour after inoculation onto barley leaves. At this stage the fungus has normally developed functional appressoria and is initiating penetration and the formation of intracellular feeding structures (haustoria).

text: Blumeria hordei DH14

text: SfilA

text: SfilB

text: DH14 20hpi

text: plasmid, Chloramphenicol resistant

text: pDNR-LIB


text: Imperial College London

text: live

text: Generic

text: Conidia (asexual spores) of Blumeria graminis f sp hordei DH14 collected from infected barley by vacuum onto Millipore filters. Conidia were not older than 24 hours.

text: Blumeria hordei DH14

text: SfilA

text: SfilB

text: DH14 Conidia

text: plasmid, Chloramphenicol resistant

text: pDNR-LIB


text: Imperial College London

text: live

text: Generic

text: Epiphytic Blumeria graminis f sp hordei DH14 collected 3 days after inoculation onto barley leaves. At this stage the epiphytic mycelium is beginning to expand on the leaf surface and penetrating epidermal cells via secondary appressoria. The colonies are barely visible to the naked eye and sporulation has not started.

text: Blumeria hordei DH14

text: SfilA

text: SfilB

text: DH14 3dpi

text: plasmid, Chloramphenicol resistant

text: pDNR-LIB


text: Imperial College London

text: live

text: Generic

text: The fruiting bodies resulting from a cross between Blumeria graminis f sp hordei DH14 and Blumeria graminis f sp hordei CC52. The cleistothecia were isolated by hand 3 months after inoculation.

text: Blumeria hordei DH14

text: SfilA

text: SfilB

text: DH14 Cleistothecia

text: plasmid, Chloramphenicol resistant

text: pDNR-LIB


text: National Center of Excellence in Molecular Biology

text: live

text: Generic

text: ESTs were obtained through differential display (Liang et. al) which appeared under CLCuV infection responsive conditions. Identified ESTs were TA cloned in PCR 2.1 vector and sequenced.

text: After one month of infection

text: LEAF

text: Gossypium hirsutum

text: Hermaphrodite

characteristic: sex

text: Total leaf tissue

characteristic: tissue-type

text: Differential display of COTTON GENOTYPE, leaf, one month of infection

text: Plasmid


characteristic: External Id

text: Genetic Engineering and Biotechnology Institute (GEBI)

characteristic: INSDC center name

text: live

text: Generic

text: Alpu 01

text: cDNA sequence from Triticum aestivum L. cv. Alpu01 at seedling stage obtained by cDNA AFLP after 150mM NaCI treatment.

text: 28 days-old seedling stage

text: Triticum aestivum

text: leaf

text: 150 mM NaCI treated leaf tissue during 27 hours


text: Chonbuk National University

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: ESTs obtained from floral organs by DD RT-PCR were cloned in modified pBluescriptII-SK plasmid.

text: early flowering

text: flower

text: Calanthe discolor

text: Calanthe discolor floral DEG Library

text: Plasmid

text: pBluescriptII-SK


text: Chonbuk National University

text: live

characteristic: INSDC status

text: Generic

text: early flowering

text: flower

text: Calanthe striata var. sieboldii

text: Calanthe sieboldii floral DEG Library

text: Plasmid

text: pBluescriptII-SK


text: University of Aveiro

text: live

text: Generic

characteristic: NCBI submission model

text: Enchytraeus albidus

text: whole body

text: Enchytraeus albidus cDNA normalized library


text: Huazhong Agricultural University

text: live

text: Generic

text: Eulaliopsis binata

text: Eulaliopsis binata full-length cDNA


text: Northeast Agricultural University

text: live

text: Generic

text: PCR-select cDNA subtraction was performed using a Clontech kit according to established protocols. The cDNA from Fusarium oxysporum inoculated seedling root was used as a tester and the cDNA from untreated seedlings root used as a driver. The cDNAs were digested with RsaI and then ligated to different adapters. Two rounds of hybridization and PCR amplification were processed to normalize and enrich the differentially expressed cDNAs. Products of the secondary PCR from the forward subtraction were purified inserted into pMD19-T Vector (Takara) and transformed into E.coli Competent Cells JM109. All the recombinant clones were picked up to establish the subtracted cDNA library.

text: After the emergence of the three primary leaves

text: Cucumis sativus

text: Root

text: Fusarium oxysporum infested cDNA library of cucumber

text: pMD19-T Vector (Takara)


text: Huazhong Agricultural University

text: live

text: Generic

text: Zinnia elegans

text: Subtracted library of Zinnia elegans between fertile and male sterile trait


text: Chulalongkorn University

text: live

text: Generic

text: The suppression subtractive cDNA library was generated as part of Penaeus monodon suppression subtractive cDNA library project. Total RNA was isolated from hemocyte of normal and YHV-infected Penaeus monodon. The mRNAs were further purified, reverse transcribed and subjected to suppression subtractive hybridization. The subtracted cDNAs were cloned into the RBC T&A Cloning Vector and then transformed into E. coli DH5 alpha.

text: E.coli DH5 alpha

text: Penaeus monodon

text: Hemocyte

text: Suppression subtractive cDNA library for YHV infection

text: Plasmid

text: RBC T&A cloning vector


text: Chulalongkorn University

text: live

text: Generic

text: The cDNA library was generated as part of Penaeus monodon EST project. Total RNA was isolated from hemocyte of Penaeus monodon. The mRNA was further purified, reverse transcribed and cloned to Uni-ZAP XR. The cDNA library in the form of single-stranded was hybridized with cDNA inserts excised from a plasmid DNA preparation of library itself. The unbound single-stranded DNA was transformed into XL-Blue.

text: E.coli SOLR strain

text: Penaeus monodon

text: EcoR I

text: Xho I

text: Gill and Epipodite

text: Gill-Epipodite normalized library

text: Phage

text: Uni-ZAP XR


text: College of Marine Science, Hainan University

text: live

text: Generic

text: head kidney cells

characteristic: cell type

text: Cloning and sequencing of the cDNA library of head kidney cells from the groupers (Epinephelus malabaricus), which was constructed by suppression subtractive hybridization (SSH). The cDNA of the head kidney cells from the groupers which were stimulated by low concentration and high purity of LPS from E. coli was used as Tester group; and the cDNA of the head kidney cells from the groupers in control group (injected with PBS) was used as Driver group.

text: The weight of the groupers was about 80 gram

text: Epinephelus malabaricus

text: malefemale

text: head kidney

text: Epinephelus malabaricus Suppression subtractive hybridization (SSH) library


text: University of Queensland St Lucia Campus

text: live

text: Generic

characteristic: NCBI submission package

text: Adult

text: Haliotis asinina

text: Cerebral and pleuropedal ganglia

text: FGHG


text: live

text: Generic

text: Adult

text: Haliotis asinina

text: Cerebral and pleuropedal ganglia

text: FGRAG


text: Dalian Fisheries University

text: live

text: Generic

text: E. coli

text: adductor muscle

text: Mizuhopecten yessoensis

text: Sfi IA

text: Sfi IB

text: muscle

text: Japanese scallop adductor muscle normalized and full-length cDNA library

text: pDNR-LIB


text: Chongqing Normal University

text: live

text: Generic

text: day 10 post-infected Bombyx mori.

characteristic: description

text: mature spores

text: CQ

characteristic: isolate

text: Nosema bombycis

text: Nosema bombycis day 10 post-infected Bombyx mori Library


text: Victorian AgriBiosciences Centre, Primary Industries Research Victoria

text: live

text: Generic

text: Blaze

characteristic: cultivar

text: Clontech SMART cDNA cloned into pGEMt Easy

text: Brassica juncea

characteristic: organism

text: Immature pods

text: Plasmid

text: pGEMt Easy


text: live

text: Generic

text: Brassica nigra

text: Immature pods

characteristic: title

text: Plasmid

text: pGEMt Easy


text: live

text: Generic

text: Blaze

text: Brassica juncea

text: Young leaves

text: Plasmid

text: pGEMt Easy

characteristic: vector


text: live

text: Generic

text: Oscar

text: Brassica napus

text: Young leaves

text: Plasmid

text: pGEMt Easy


text: live

text: Generic

text: Brassica nigra

text: Young leaves

text: Plasmid

text: pGEMt Easy


text: live

text: Generic

text: Oscar

text: Brassica napus

text: Old leaves

text: Plasmid

text: pGEMt Easy


text: live

text: Generic

text: Blaze

text: Brassica juncea

text: Old leaves infected with Leptosphaeria maculans PG4

text: Plasmid

text: pGEMt Easy


text: live

text: Generic

text: Westar

text: Brassica napus

text: Cotyledon

text: Plasmid

text: pGEMt Easy


text: live

text: Generic

text: Surpass 400

text: Brassica napus

text: Cotyledon inoculated with Leptosphaeria maculans strain M1

text: Plasmid

text: pGEMt Easy


text: live

text: Generic

text: Surpass 400

text: Brassica napus

text: Cotyledon

text: Plasmid

text: pGEMt Easy


text: live

text: Generic

text: Surpass 400

text: Brassica napus

text: 14 day old leaves inoculated with Leptosphaeria maculans strain M1

text: Plasmid

text: pGEMt Easy


text: live

text: Generic

text: Surpass 400

text: Brassica napus

text: 14 day old leaves

text: Plasmid

text: pGEMt Easy


text: live

text: Generic

text: Surpass 400

text: Brassica napus

text: root tissue field infection of Leptosphaeria maculans

text: Plasmid

text: pGEMt Easy


text: live

text: Generic

text: Av Sapphire

text: Brassica napus

text: Plasmid

text: pGEMt Easy


text: Central Institute of Medicinal and Aromatic Plants (CIMAP, CSIR)

text: live

text: Generic

text: PCR based suppression subtractive hybridization was carried out using the cDNA populations derived from the poly (A)+ mRNA isolated from the leaf (tester) and root (driver) tissues of an eighteen week old Withania somnifera plant growing in the CIMAP farm at Lucknow under the National Gene Bank for Medicinal and Aromatic Plants and the subtracted cDNA population was cloned on pGEM-T Easy vector.

text: Eighteen week old field-growing plant

characteristic: dev-stage

text: Leaf

characteristic: organ

text: Withania somnifera

text: Withania somnifera subtracted leaf-specific cDNA library

text: Plasmid

text: pGEM-T Easy


text: live

text: Generic

text: PCR based suppression subtractive hybridization was carried out using the cDNA populations derived from the poly (A)+ mRNA isolated from the root (tester) and leaf (driver) tissues of an eighteen week old Withania somnifera plant growing in the CIMAP farm at Lucknow under the National Gene Bank for Medicinal and Aromatic Plants and the subtracted cDNA population was cloned on pUC19 vector.

text: Eighteen week old field-growing plant

text: Root

text: Withania somnifera

text: Withania somnifera subtracted root-specific cDNA library

text: Plasmid


text: Cardiff University

text: live

text: Generic

text: Rebecca

text: COLD

text: Alstroemeria peruviana

text: petal

characteristic: tissue-type

text: Alstroemeria peruviana petal COLD


characteristic: External Id

text: Cardiff University

text: live

text: Generic

text: Rebecca

text: DRY

text: Alstroemeria peruviana

text: petal

text: Alstroemeria peruviana petal DRY


text: Cardiff University

characteristic: INSDC center name

text: live

text: Generic

text: Rebecca

text: Alstroemeria peruviana

text: petal

text: Alstroemeria peruviana petal S0S1


text: Cardiff University

characteristic: INSDC first public

text: live

text: Generic

text: Rebecca

text: Alstroemeria peruviana

text: petal

text: Alstroemeria peruviana petal S2S4


text: Cardiff University

characteristic: INSDC last update

text: live

text: Generic

text: Rebecca

text: Alstroemeria peruviana

text: petal

text: Alstroemeria peruviana petal S5S7


text: Cardiff University

text: live

characteristic: INSDC status

text: Generic

text: Rebecca

text: Alstroemeria peruviana

text: petal

text: Alstroemeria peruviana petal SSH0-2


text: Cardiff University

text: live

text: Generic

characteristic: NCBI submission model

text: Rebecca

text: Alstroemeria peruviana

text: petal

text: Alstroemeria peruviana petal SSH2-0


text: Cardiff University

text: live

text: Generic

text: Rebecca

text: Alstroemeria peruviana

text: petal

text: Alstroemeria peruviana petal SSH2-45


text: Cardiff University

text: live

text: Generic

text: Rebecca

text: Alstroemeria peruviana

text: petal

text: Alstroemeria peruviana petal SSH3-2


text: Cardiff University

text: live

text: Generic

text: Rebecca

text: Alstroemeria peruviana

text: petal

text: Alstroemeria peruviana petal SSH45-2


text: Anhui Agricultural University

text: live

text: Generic

characteristic: cell-line

text: Camellia sinensis

text: hermaphrodite

characteristic: sex

text: callus

text: Callus SSH cDNA library of tea plant (Camellia sinensis) induced by light


text: AgResearch

text: live

text: Generic

characteristic: NCBI submission package

text: SSH reverse subtraction of Lolium perenne mature tissue infected with Neotyphodium lolii versus uninfected.

characteristic: description

text: Lolium perenne/Neotyphodium lolii mixed EST library

text: E-M

text: plasmid


text: AgResearch

text: live

text: Generic

text: SSH reverse subtraction of Lolium perenne 9 day seedling tissue infected with Neotyphodium lolii versus uninfected.

text: E-S

text: plasmid


text: AgResearch

text: live

text: Generic

text: SSH forward subtraction of Lolium perenne mature tissue infected with Neotyphodium lolii versus uninfected.

text: E+M

text: plasmid


text: AgResearch

text: live

text: Generic

text: SSH forward subtraction of Lolium perenne 9 day seedling tissue infected with Neotyphodium lolii versus uninfected.

text: E+S

text: plasmid


text: National Institute for Plant Genome Research

text: live

text: Generic

text: PRASAD

characteristic: cultivar

text: 21 days old seedlings

text: Setaria italica

text: SSH library of dehydration stress in cv. Prasad (Setaria italica L.)


text: College of Natural Resource and Environment, South China Agricultural University

text: live

text: Generic

text: adult

text: Bactrocera dorsalis

characteristic: organism

text: male and female

text: foreleg

text: T7 phage display cDNA library of foreleg from Bactrocera dorsalis Hendel


text: IPATIMUP Institute of Pathology and Immunology, University of Porto

text: live

text: Generic

text: Testis RACE-ready cDNA derived from testis total RNA Ambion (cat. #AM6000)

text: Homo sapiens

text: male

text: testis

text: Testis RACE-ready cDNA

characteristic: title


text: Universidad de Malaga, Facultad de ciencias

text: live

text: Generic

text: ecotype: Corsican; The subtractive hybridization protocol from BD Biosciences Clontech (Palo Alto, CA) was used to generate cDNA libraries 3, 4, 13 and 14. Differentiating xylem samples for library 3 and 4 were collected from the compression and opposite sides of a twelve-year-old artificially bent tree (15 degree away from the vertical position). For library 13 and 14, juvenile and mature differentiating xylem were sampled from the crown and the base of a 30-year-old tree. Library 3 (tester: opposite xylem / driver: compression xylem), library 4 (tester: compression xylem / driver: opposite xylem), library 13 (tester: juvenile xylem / driver: mature xylem), library 14 (tester: mature xylem / driver: juvenile xylem).

text: Pinus pinaster

text: differentiating xylem

text: Pinus pinaster differentiating xylem libraries 3, 4, 13, 14


text: Oklahoma State University

text: live

text: Generic

text: Amblyomma americanum ticks were obtained from the laboratory colony maintained at the Oklahoma State University Tick Rearing Facility. Total RNA was extracted from 375 whole unfed adult ticks (150 males and 225 females) using the RNAeasy kit (Qiagen, Valencia, CA, USA). The cDNA library was synthesized using the CloneMiner cDNA Library Construction Kit in the vector pDONR222 (Invitrogen, Carlsbad, CA, USA). The library had >90% recombinants with inserts >250 bp.

text: unfed adults

text: Amblyomma americanum

text: female and male

text: OSU colony

characteristic: strain

text: whole ticks

text: Unfed adult Amblyomma americanum tick cDNA Library

characteristic: vector


text: College of Life Science and Biotechnology, Mianyang Normal University

text: live

text: Generic

text: wild Conocephalum conicum collected in Xiao Zhai Zi Gou nature reserve of Sichuan province in China.

text: Conocephalum conicum

text: SfiIA

text: SfiIB

text: hermaphrodite

text: thallus

text: Conocephalum conicum full-length cDNA library

text: Phage


text: IRTA

text: live

text: Generic

text: Normalised master library formed by a mixture of three cDNA libraries from embryos exposed ro water or air and at hatching stage. Each library was constructed using the SMART cDNA Library Construction kit (BD Biosciences Clontech) Embryos cultured in water or exposed to air at 2-4 cells, morula, blastula, gastrula (50% epiboly), neurula (24h), 48h, 5 days postfertilization (dpf), 7 dpf, 14 dpf.

text: embryos, mixed

characteristic: dev-stage

text: Fundulus heteroclitus

text: SfiI

text: SfiI

text: Mix of cDNA libraries from embryos


text: Cummings School of Veterinary Medicine at Tufts University

text: live

text: Generic

text: most cell types

characteristic: cell type

text: Whole postlarvae stage 42 (PL42) from cultured, specific pathogen-free Litopenaeus vannamei shrimp from the Kona line (also called 'Research Line' or 'TSV-susceptible Line') of the US Marine Shrimp Farming Program. The CLONTECH PCR-SMARTTM cDNA kit was used for library construction.

text: postlarvae stage 42

text: E. coli (ElectroMAX DH5a-E

characteristic: lab-host

text: whole postlarvae

characteristic: organ

text: Penaeus vannamei

text: Sfi A (5'end)

text: Sfi B (3'end)

text: most tissue types

characteristic: tissue-type

text: pDNR-LIB (CLONETECH)


characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: most cell types

text: Whole postlarvae stage 42 (PL42) from cultured, specific pathogen-free Litopenaeus vannamei shrimp from the Kona line (also called 'Research Line' or 'TSV-susceptible Line') of the US Marine Shrimp Farming Program was used. Postlarvae were exposed to cadmium (1ppm) for 24 hours. The CLONTECH PCR-SMARTTM cDNA kit was used for library construction.

text: postlarvae stage 42

text: E. coli (ElectroMAX DH5a-E

text: whole postlarvae

text: Penaeus vannamei

text: Sfi A (5'end)

text: Sfi B (3'end)

text: most tissue types

text: pDNR-LIB (CLONETECH)


text: Baylor College of Medicine

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: periferal blood leukocytes

text: oligo Dt priming from poly A+ RNA, directionally cloned

text: adult

text: E.coli

text: Tursiops truncatus

text: SfilA

text: SfilB

text: blood

text: Bottlenose dolphin IL-2-stimulated periferal blood leukocyte cDNA library


text: Baylor College of Medicine

text: live

text: Generic

characteristic: NCBI submission package

characteristic: description

text: adult

text: E.coli

text: liver

text: Tursiops truncatus

text: SfilA

text: SfilB

text: female

characteristic: sex

text: Bottlenose dolphin liver cDNA library


text: Baylor College of Medicine

text: live

text: Generic

text: adult

text: E.coli

text: spleen

text: Tursiops truncatus

characteristic: organism

text: SfilA

text: SfilB

text: Bottlenose dolphin spleen cDNA library

characteristic: title


text: Baylor College of Medicine

text: live

text: Generic

text: adult

text: E.coli

text: kidney

text: Tursiops truncatus

text: SfilA

text: SfilB

text: female

text: Bottlenose dolphin kidney cDNA library

characteristic: vector


text: Baylor College of Medicine

text: live

text: Generic

text: adult

characteristic: dev-stage

text: E.coli

text: Tursiops truncatus

text: SfilA

text: SfilB

text: male

text: muscle

text: Bottlenose dolphin muscle cDNA library


text: Baylor College of Medicine

text: live

text: Generic

text: adult

text: E.coli

text: skin

text: Tursiops truncatus

text: SfilA

text: SfilB

text: Bottlenose dolphin skin cDNA library


text: Scottish Crop Research Institute

text: live

text: Generic

text: Latham

characteristic: cultivar

text: Sequenced with universal M13 reverse primer

text: Rubus idaeus subsp. strigosus

text: Roots

text: Red raspberry root library


text: International Graduate School (IHI) Zittau

text: live

text: Generic

text: Fungus was grown in plant-based complex medium based on tomato juice and distilled water (50:50 v/v) at 24°C and 100 rpm. Biomass pellets were harvested at different culture days by centrifugation and frozen at -80°C. Total RNA was extracted using Trizol reagent (Invitrogen) and purified via phenol-chloroform extraction and ethanol precipitation. For cDNA synthesis, total RNA (1 µg) was primed by using a poly(dT)-anchor primer (5'-TGTAACCGCGTATCAGTGCTTTTTTTTTTTTTTTTT-3'). Afterward, the total mRNA was reversely transcribed into cDNA with the anchor sequence added to the 3' end using a ?RevertAid H Minus First Strand cDNA Synthesis Kit? (Fermentas).

text: different culture days

text: Auricularia auricula-judae

text: DSMZ 11326

characteristic: strain

text: Pellets obtained by agitated liquid culture in flask

text: Auricularia cultivation in tomato juice medium


text: University of Southern Denmark

text: live

text: Generic

text: cDNA produced with Roche cDNA Synthesis System (Cat# 11117831001), and 5´-phosphorylated with polynucleotide kinase. The cDNA was cloned into SmaI-digested, de-phosphorylated pUC18.

text: Young adult

text: Tentacle

characteristic: organ

text: Octopus vulgaris

text: SmaI

text: Octopus vulgaris, pUC18 library

text: Plasmid


text: Institut Pasteur, Paris

text: live

text: Generic

text: Entamoeba histolytica

text: E. histolytica cDNA library cloned in p4440 plasmid


text: Institut de Recherche pour le Developpement

text: live

text: Generic

text: Suppression subtractive hybridization library, mantled male inflorescence was subtracted against normal male inflorescence

text: immature

characteristic: lab-host

text: male inflorescence

text: Elaeis guineensis

text: Clonal line FC166

text: InfSSHM1, normal male inflorescence

text: plasmid

text: pGEM-T Easy


characteristic: External Id

text: live

text: Generic

text: immature

text: male inflorescence

text: Elaeis guineensis

text: Clonal line FC166

text: InfSSHM2, mantled male inflorescence

text: plasmid

text: pGEM-T Easy


text: Washington State University

characteristic: INSDC center name

text: live

text: Generic

text: Chlorenchyma cell

characteristic: cell type

text: Construct library using mRNA of chlorenchyma cells of Bienertia sinuspersici.

text: Mature leaf

text: Bienertia sinuspersici

text: Chlorenchyma cells of Bienertia sinuspersici


text: School of Life Sciences, Xiamen University

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Male Sebastiscus marmoratus weighing 23-27 g, were captured from coastal area in Xiamen city, Fujian province, China. Before the exposure experiment, the fish were acclimated in tanks (25 fish in each tank) containing 50 L of aerated sand-filtered seawater, with natural photoperiod for 7 days. Fish were dosed via single intraperitoneal injection with TBT (500¦Ìg/kg body weight, dissolved in corn oil) or with only corn oil as a control. All groups were injected two times, and injections were repeated interval 3 days. After 1 week exposure, fish were randomly sampled from each group. The isolated brains were frozen in liquid N2 and immediately stored at -80¡æ until analysis.For the ACP-ddRT-PCR procedure, 20 arbitrary and 2 dT primers were designed for this study, the total RNA for analysis was extracted from 10 brains homogenate.After ddRT-PCR analyze, the differentially expressed bands were extracted and cloned into pMD18-T vector, enriched for differential display cDNA library in response to TBT.

text: Adult

text: E.coli TOP10

text: Brain

text: Sebastiscus marmoratus

text: Male

text: Anneal Control Primer-based Differential Display cDNA library for brain of Sebastiscus marmoratus in response to TBT

text: Plasmid


text: Cornell University

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: mature

text: Individual isolate from which the sequence was obtained

characteristic: isolate

text: Laupala cerasina

text: male

text: L. cerasina minus L. eukolea subtractive library


text: Cornell University

text: live

text: Generic

characteristic: NCBI submission package

text: mature

text: Laupala eukolea

text: male

characteristic: sex

text: L. eukolea minus L. cerasina subtractive library


text: Department of Fisheries and Oceans

text: live

text: Generic

text: April 3rd, 2008, whole ears were isolated from individual juvenile Atlantic salmon smolt that were exposed 16 hours earlier to 50 seismic blasts using a 10 cubic inch airgun with received levels at 202 dB peak-to-peak (relative to 1uPa). A control group of juvenile Atlantic salmon smolt, siblings of the exposed group, was handled in the same manner except without exposure to seismic blasts. Total RNA was isolated from individual Atlantic salmon ear taken from the left lateral side, by homogenization in TRIzol Reagent (Invitrogen) following manufacturer's instructions. Total RNA was treated with DNase-I (RNase-Free DNase Set, Qiagen) and column purified (RNeasy MinElute Clean-up Kit, Qiagen). DNase-I treated, cleaned total RNA (4.0 micrograms per individual) was pooled from 12 individual fish per pool (seismic exposed pool, non-exposed control pool) followed by poly(A+)RNA (mRNA) isolation from each total RNA pool (MicroPoly (A) Purist Small Scale mRNA Purification Kit, Ambion). Poly(A+)RNA from seismic exposed and non-exposed control pools were used for constructing forward (designed to be enriched for ear transcripts up-regulated by seismic sound exposure) and reverse (designed to be enriched for ear transcripts down-regulated by seismic sound exposure) suppression subtractive hybridization (SSH) cDNA libraries using PCR-Select cDNA Subtraction Kit and Advantage 2 Polymerase Mix (Clontech). SSH cDNA libraries were purified using MinElute PCR Purification Kit (Qiagen). A high molecular weight sub-library was generated by excising the 500-1200 bp gel fraction from the PCR product generated from the forward and reverse libraries, followed by purification using QIAquick Gel Extraction Kit (Qiagen). SSH libraries were cloned into pGEM-T Easy Vector (Promega) and transformed using ElectroMAX DH5alpha-E Cells (Invitrogen). Extracted and purified DNA from individual bacterial clones was sequenced using Applied Biosystems Big Dye chemistry on ABI 3730 DNA Analyzer (equipped with a 48-capillary array). Expressed sequence tags (ESTs) were sequenced in 96-well plate format, with EST numbers designated by row (uppercase A-H) and column number (1-12), followed by plate number (lowercase p) 1-10. Of the 333 ESTs generated from the forward SSH library (ssal_ear_SSH_seismicF), 176 ESTs (average length 664 bp) came from a size-fractionated (500-1200 bp) sub-library (plate 2 wells A7-H12; all of plates 5 and 6) and 157 ESTs (average length 435 bp) came from a non-size-fractionated library (plate 1 wells B3-H3 and D7-H9; all of plates 7 and 8).

characteristic: description

text: Salmo salar

characteristic: organism

text: EcoRI

text: EcoRI

text: juvenile

text: ear

characteristic: tissue-type

text: Salmo salar ear forward SSH library designed to be enriched for transcripts up-regulated by seismic sound.

characteristic: title

text: plasmid

text: pGEM-T easy


text: Department of Fisheries and Oceans

text: live

text: Generic

text: April 3rd, 2008, whole ears were isolated from individual juvenile Atlantic salmon smolt that were exposed 16 hours earlier to 50 seismic blasts using a 10 cubic inch airgun with received levels at 202dB peak-to-peak (relative to 1uPa). A control group of juvenile Atlantic salmon smolt, siblings of the exposed group, was handled in the same manner except without exposure to seismic blasts. Total RNA was isolated from individual Atlantic salmon ear taken from the left lateral side, by homogenization in TRIzol Reagent (Invitrogen) following manufacturer's instructions. Total RNA was treated with DNase-I (RNase-Free DNase Set, Qiagen) and column purified (RNeasy MinElute Clean-up Kit, Qiagen). DNase-I treated, cleaned total RNA (4.0 micrograms per individual) was pooled from 12 individual fish per pool (seismic exposed pool, non-exposed control pool) followed by poly(A+)RNA (mRNA) isolation from each total RNA pool (MicroPoly (A) Purist Small Scale mRNA Purification Kit, Ambion). Poly(A+)RNA from seismic exposed and non-exposed control pools were used for constructing forward (designed to be enriched for ear transcripts up-regulated by seismic sound exposure) and reverse (designed to be enriched for ear transcripts down-regulated by seismic sound exposure) suppression subtractive hybridization (SSH) cDNA libraries using PCR-Select cDNA Subtraction Kit and Advantage 2 Polymerase Mix (Clontech). SSH cDNA libraries were purified using MinElute PCR Purification Kit (Qiagen). A high molecular weight sub-library was generated by excising the 500-1200 bp gel fraction from the PCR product generated from the forward and reverse libraries, followed by purification using QIAquick Gel Extraction Kit (Qiagen). SSH libraries were cloned into pGEM-T Easy Vector (Promega) and transformed using ElectroMAX DH5alpha-E Cells (Invitrogen). Extracted and purified DNA from individual bacterial clones was sequenced using Applied Biosystems Big Dye chemistry on ABI 3730 DNA Analyzer (equipped with a 48-capillary array). Expressed sequence tags (ESTs) were sequenced in 96-well plate format, with EST numbers designated by row (uppercase A-H) and subsequent column number (1-12), followed by plate number (lowercase p) 1-10. Of the 350 ESTs generated from the reverse SSH library (ssal_ear_SSH_seismicR), 201 ESTs (average length 706 bp) came from a size-fractionated (500-1200 bp) sub-library (plate 2 wells A1-H6; all of plates 3 and 4) and 149 ESTs (average length 525 bp) came from a non-size-fractionated library (plate 1 wells A1-A3 and A5-C7; all of plates 9 and 10).

text: Salmo salar

text: EcoRI

text: EcoRI

text: juvenile

text: ear

text: Salmo salar ear reverse SSH library designed to be enriched for transcripts down-regulated by seismic sound.

text: plasmid

text: pGEM-T easy

characteristic: vector


text: National Research Centre for Banana, Trichy

text: live

text: Generic

text: Manoranjitham

characteristic: cultivar

text: Four months old tissue cultured Musa accuminata cultivars Manoranjitham (AAA) and Robusta (AAA) were challenged with spores of Mycospharella eumusae, cDNA was synthesized from the pooled polyA+ RNA obtained from leaf tissues collected at every 6hours up to 66hrs post infection. Suppressive subtractive hybridization was performed with the two pools of cDNA obtained from the cultivars namely cv. Manoranjitham as tester and cv. Robusta as driver. The subtracted cDNA fragments were cloned into TA cloning vector pGEMT-Easy and sequenced.

text: 4 months old tissue cultured plants

characteristic: dev-stage

text: Musa acuminata AAA Group

text: Eco RI

text: Eco RI

text: Hermaphordite

text: Leaf

text: Musa accuminata cv. Manoranjitham Leaf Tissue Specific Library

text: plasmid

text: pGEMT-Easy


characteristic: External Id

text: University of Kentucky

text: live

text: Generic

text: RNA extracted from whole animals, or from specific tissues was used to construct cDNA libraries from which ESTs were generated.

text: mixed, larval, adult

text: E. coli

characteristic: lab-host

text: Heliothis virescens

text: mixed tissues, whole insects, midgut, nervous system

text: Heliothis virescens cDNA Libraries

text: plasmid


text: ETH Zurich

characteristic: INSDC center name

text: live

text: Generic

text: Silene latifolia

text: Silene latifolia, enriched library

text: pGEM-T vector


text: National University of Ireland, Galway

characteristic: INSDC first public

text: live

text: Generic

text: Suppression subtractive hybridization library

text: February smolt vs May smolt

text: Salmo salar

text: Intestine

text: Intestine SSH reverse February smolt vs May smolt


text: National University of Ireland, Galway

characteristic: INSDC last update

text: live

text: Generic

text: February smolt vs May smolt

text: Salmo salar

text: Intestine

text: Intestine SSH forward February smolt vs May smolt


text: National University of Ireland, Galway

text: live

characteristic: INSDC status

text: Generic

text: February smolt vs May smolt

text: Salmo salar

text: Head Kidney

text: Head Kidney SSH forward February smolt vs May smolt


text: National University of Ireland, Galway

text: live

text: Generic

characteristic: NCBI submission model

text: February smolt vs May smolt

text: Salmo salar

text: Head Kidney

text: Head Kidney SSH reverse February smolt vs May smolt


text: National University of Ireland, Galway

text: live

text: Generic

text: February smolt vs May smolt

text: Salmo salar

text: Spleen

text: Spleen SSH forward February smolt vs May smolt


text: National University of Ireland, Galway

text: live

text: Generic

text: February smolt vs May smolt

text: Salmo salar

text: Spleen

text: Spleen SSH reverse February smolt vs May smolt


text: National University of Ireland, Galway

text: live

text: Generic

text: February smolt vs April smolt

text: Salmo salar

text: Brain

text: Brain SSH forward Parr vs Precocious Parr


text: National University of Ireland, Galway

text: live

text: Generic

text: February smolt vs April smolt

text: Salmo salar

text: Brain

text: Brain SSH reverse Parr vs Precocious Parr


text: National University of Ireland, Galway

text: live

text: Generic

text: Parr vs Precocious Parr

text: Salmo salar

text: Testis

text: Testis SSH reverse Parr vs Precocious Parr


text: National University of Ireland, Galway

text: live

text: Generic

text: Maturing adults

text: Salmo salar

text: Ovary

text: Ovary SSH forward Maturing adults


text: National University of Ireland, Galway

text: live

text: Generic

text: Maturing adults

text: Salmo salar

text: Ovary

text: Ovary SSH reverse Maturing adults


text: National University of Ireland, Galway

text: live

text: Generic

text: February smolt vs April smolt

text: Salmo salar

text: Pituitary

text: Pituitary SSH forward February smolt vs April smolt


text: National University of Ireland, Galway

text: live

text: Generic

text: February smolt vs April smolt

text: Salmo salar

text: Pituitary

text: Pituitary SSH reverse February smolt vs April smolt


text: National University of Ireland, Galway

text: live

text: Generic

text: Non-Precocious vs Precocious

text: Salmo salar

text: Pituitary

text: Pituitary SSH forward Non-Precocious vs Precocious


text: National University of Ireland, Galway

text: live

text: Generic

text: Non-Precocious vs Precocious

text: Salmo salar

text: Pituitary

text: Pituitary SSH reverse Non-Precocious vs Precocious


text: National University of Ireland, Galway

text: live

text: Generic

text: April smolt vs Sea smolt

text: Salmo salar

text: Pituitary

text: Pituitary SSH forward April smolt vs Sea smolt


text: National University of Ireland, Galway

text: live

text: Generic

text: April smolt vs Sea smolt

text: Salmo salar

text: Pituitary

text: Pituitary SSH reverse April smolt vs Sea smolt


text: National University of Ireland, Galway

text: live

text: Generic

text: Parr vs Grilse

text: Salmo salar

text: Female

text: Pituitary

text: Pituitary SSH forward Parr vs Grilse


text: National University of Ireland, Galway

text: live

text: Generic

text: Parr vs Grilse

text: Salmo salar

text: Female

text: Pituitary

text: Pituitary SSH reverse Parr vs Grilse


text: National University of Ireland, Galway

text: live

text: Generic

text: Grilse vs Fully mature Adult

text: Salmo salar

text: Female

text: Pituitary

text: Pituitary SSH forward Grilse vs Fully mature Adult


text: National University of Ireland, Galway

text: live

text: Generic

text: Suppression subtractive hybridization library

text: Grilse vs Fully mature Adult

text: Salmo salar

text: Female

text: Pituitary

text: Pituitary SSH reverse Grilse vs Fully mature Adult


text: National University of Ireland, Galway

text: live

text: Generic

text: Post-blastula vs Pre-blastula

text: Salmo salar

text: Embryo

text: Embryo SSH reverse Post-blastula vs Pre-blastula


text: National University of Ireland, Galway

text: live

text: Generic

text: February smolt vs April smolt

text: Salmo salar

text: Hypothalamus

text: Hypothalamus SSH forward February smolt vs April smolt


text: National University of Ireland, Galway

text: live

text: Generic

text: February smolt vs April smolt

text: Salmo salar

text: Hypothalamus

text: Hypothalamus SSH reverse February smolt vs April smolt


text: National University of Ireland, Galway

text: live

text: Generic

text: parr vs precocious parr

text: Salmo salar

text: Hypothalamus

text: Hypothalamus SSH forward parr vs precocious parr


text: National University of Ireland, Galway

text: live

text: Generic

text: parr vs precocious parr

text: Salmo salar

text: Hypothalamus

text: Hypothalamus SSH reverse parr vs precocious parr


text: National University of Ireland, Galway

text: live

text: Generic

text: parr vs fully mature

text: Salmo salar

text: female

text: Hypothalamus

text: Hypothalamus SSH forward parr vs fully mature


text: National University of Ireland, Galway

text: live

text: Generic

text: parr vs fully mature

text: Salmo salar

text: female

text: Hypothalamus

text: Hypothalamus SSH reverse parr vs fully mature


text: National University of Ireland, Galway

text: live

text: Generic

text: April smolt vs May smolt

text: Salmo salar

text: Gill

text: Gill SSH forward April smolt vs May smolt


text: National University of Ireland, Galway

text: live

text: Generic

text: April smolt vs May smolt

text: Salmo salar

text: Gill

text: Gill SSH reverse April smolt vs May smolt


text: National University of Ireland, Galway

text: live

text: Generic

text: February smolt vs April smolt

text: Salmo salar

text: Brain

text: Brain SSH forward February smolt vs April smolt


text: National University of Ireland, Galway

text: live

text: Generic

text: February smolt vs April smolt

text: Salmo salar

text: Brain

text: Brain SSH reverse February smolt vs April smolt


text: National University of Ireland, Galway

text: live

text: Generic

text: Salmo salar

text: Testis

text: Testis SSH forward


text: Ecole Nationale d'Ingenieurs de Sfax

text: live

text: Generic

text: Nicola

text: cDNA encoding for ERF (ETHYLENE RESPONSE FACTOR) were isolated from potato (Solanum tuberosum) leaves under salt stress treatment for 24 hours, by RT-PCR. cDNA-ERF were inserted into pDONR207 vector using Gateway technology.

text: Leaf

characteristic: organ

text: Solanum tuberosum

text: PstI

text: AvaI

text: Solanum tuberosum ERFs

text: Plasmid

text: pDONR207 Vector (invitogen)


text: The Johns Hopkins University

text: live

text: Generic

characteristic: NCBI submission package

text: Brain cDNA Library, Suppression Subtraction Hybridization PCR, enriched for differentially expressed transcripts resulting from oral exposure to cyclotrimethylenetrinitramine (RDX).

text: Pimephales promelas

text: Brain

text: Fathead minnow brain cDNA Library


text: Universite Claude Bernard Lyon 1

text: live

text: Generic

text: country: France:North East France, Nievre-Morvan, Breuil Chenue forest; environmental_sample; lat_lon: 47.18 N 4.4 E

text: uncultured eukaryote

text: uncultured eukaryote from sandy-clay soil from a Fagus sylvatica forest


text: National Institute of Biomedical Innovation

text: live

text: Generic

text: 1st strand cDNA was primed with an oligo(dT) primer [ATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded using specific 5' and 3' primers and amplified by PCR. The PCR cDNA was synthesized product was digested with SfiI and size selection was performed to exclude fragments <1.5kb. The SfiI-digested PCR product was cloned into distinct DraIII sites of pME18S-FL3. XhoI sites just outside the DraIII sites can be used to isolate the cDNA insert. Libraries were constructed by oligo-capping method.

text: 3 years old

text: Macaca fascicularis

text: DraIII (CACTGTGTG)

text: DraIII (CACCATGTG)

text: male

characteristic: sex

text: heart

text: macaque heart cDNA library QhtB

text: pME18S-FL3 (Acc.No. AB009864)


text: University of Malaga

text: live

text: Generic

text: Carisma

text: Unripped Berries (F7), green fruit

text: E. coli

text: Fruit

text: Fragaria x ananassa

text: EcoRI

text: XhoI

text: Receptacle

text: Fragaria x ananassa var. Carisma green fruit receptacle UMA library M1

text: Plasmid

text: pBK-CMV


text: University of Malaga

text: live

text: Generic

text: Carisma

text: Unrippened Berries (F3), green fruit

text: E. coli

text: Fruit

text: Fragaria x ananassa

text: EcoRI

text: XhoI

text: Achene

text: Fragaria x ananassa var. Carisma green fruit achenes UMA library M2

text: Plasmid

text: pBK-CMV


text: University of Malaga

text: live

text: Generic

text: Elsanta

text: Red fruits treated with ethylene

characteristic: description

text: Ripe Berries (F7), red fruit

text: E. coli

text: Fruit

text: Fragaria x ananassa

text: Achenes and Receptacles

text: Fragaria x ananassa var. Elsanta red fruit ethylene treated library L1

text: Plasmid

text: pGEM-T-Easy


text: Ningbo University

text: live

text: Generic

text: Larimichthys crocea

characteristic: organism

text: skeletal muscle

text: large yellow croaker skeletal muscle cDNA library

text: Plasmid

text: pDNR-LIB


text: Institute of Himalayan Bioresource Technology

text: live

text: Generic

text: Leaf

text: Sinopodophyllum hexandrum

text: Leaf

characteristic: tissue-type

text: Podophyllum hexandrum cDNA library

text: plasmid

text: pGEM-T Easy (Promega)


text: live

text: Generic

text: Agropyron mongolicum

text: Agropyron mongolicum Keng Transcript-derived fragment

characteristic: title


text: National Institute of Agrobiological Sciences

text: live

text: Generic

text: wandering larvae

text: Ostrinia nubilalis

text: epidermis

text: Ostrinia nubilalis wandering larval epidermis cDNA library


text: live

text: Generic

text: pupae

text: Ostrinia nubilalis

text: whole body

text: Ostrinia nubilalis pupal whole body cDNA library


text: live

text: Generic

text: adult

text: Ostrinia nubilalis

text: female

text: pheromone gland

text: Ostrinia nubilalis female adult pheromone gland cDNA library


text: live

text: Generic

text: adult

text: Ostrinia nubilalis

text: male

text: antenna

text: Ostrinia nubilalis male adult antenna cDNA library


text: live

text: Generic

text: pharate pupae

text: Ostrinia nubilalis

text: midgut

text: Ostrinia nubilalis pharate pupal midgut cDNA library


text: live

text: Generic

text: wandering larvae

text: Ostrinia nubilalis

text: hemocyte

text: Ostrinia nubilalis wandering larval hemocyte cDNA library


text: live

text: Generic

text: pharate pupae

text: Ostrinia nubilalis

text: fat body

text: Ostrinia nubilalis pharate pupal fat body cDNA library


text: live

text: Generic

text: last instar larvae

text: Ostrinia nubilalis

text: middle silk gland

text: Ostrinia nubilalis last instar larval middle silk gland cDNA library


text: live

text: Generic

text: wandering larvae

text: Ostrinia nubilalis

text: Malpighian tubule

text: Ostrinia nubilalis wandering larval Malpighian tubule cDNA library


text: live

text: Generic

text: embyro within 1 day after oviposition

text: Ostrinia nubilalis

text: embryo

text: Ostrinia nubilalis day 0 embryonic cDNA library


text: live

text: Generic

text: adult

text: Ostrinia nubilalis

text: female

text: antenna

text: Ostrinia nubilalis female adult antenna cDNA library


text: live

text: Generic

text: adult

text: Ostrinia nubilalis

text: male

text: hair pencil

text: Ostrinia nubilalis male adult hair pencil cDNA library


text: The University of Tokyo, Department of Parasitology

text: live

text: Generic

text: erythrocytic stage

text: Plasmodium vivax

text: XPV Sugano cDNA library


text: live

text: Generic

text: erythrocytic stage

text: Plasmodium yoelii

characteristic: strain

text: XPYs Sugano cDNA library


text: live

text: Generic

text: erythrocytic stage

text: Plasmodium yoelii

text: XPYw Sugano cDNA library


text: live

text: Generic

text: erythrocytic stage

text: Plasmodium berghei

text: ANKA

text: XPB Sugano cDNA library


text: live

text: Generic

text: sporozoite stage

text: Cryptosporidium parvum

text: XCP Sugano cDNA library


text: live

text: Generic

text: tachyzoite stage

text: Toxoplasma gondii

text: RH

text: XTG Sugano cDNA library


text: live

text: Generic

text: erythrocytic stage

characteristic: isolate

text: Plasmodium falciparum 3D7

text: XPF Sugano cDNA library


text: University of Richmond

text: live

text: Generic

text: Aedes atropalpus

text: Autogenous SSH library

text: Plasmid

text: pCRIV-TOPO (Invitrogen)

characteristic: vector


characteristic: External Id

text: Indain Institute of Integrative Medicine

text: live

text: Generic

text: WSR

characteristic: cultivar

text: Total RNA extracted from leaf tissue and subsequently constructed into cDNA library using Gigapack Gold (Stratagene) Kit.

text: Mature plant

characteristic: dev-stage

text: Withania somnifera

text: Bisexual

text: Leaf

text: Withania somnifera WSR leaf tissue cDNA library

text: UNI_ZAP Lambda expression vector


text: Max Planck Institute for Chemical Ecology

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: Polygonia c-album GeneFishing differential expression analysis

text: Gut

text: Nymphalis c-album

text: male and female

text: Midgut

text: Pca_G GeneFishing

text: Plasmid

text: pCR 2.1 TOPO


characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Carcass

text: Nymphalis c-album

text: male and female

text: Larval body

text: Pca_N GeneFishing

text: Plasmid

text: pCR 2.1 TOPO


text: National Research Center on Plant Biotechnology (NRCPB)

text: live

text: Generic

characteristic: NCBI submission model

text: Nagina 22

text: collected_by: Sangram Keshari Lenka; collection_date: 09-july-2007; country: India

text: Oryza sativa Indica Group

text: Leaf and root

text: Oryza sativa Indica Group Nagina 22 leaf and root


text: National Institute of Allergy and Infectious Diseases

text: live

text: Generic

characteristic: NCBI submission package

text: unidirectional library was constructed using long distance PCR (Smart cDNA kit) and Lambda TriplEx2 vector

text: adult female

text: salivary glands

characteristic: organ

text: Simulium nigrimanum

text: female

text: Adult female salivary gland cDNA library Simnigri1

text: Phage

text: Lambda TriplEx2


text: University of Maine

text: live

text: Generic

text: Library for ESTs whose expression is up-regulated in juvenile mussels 23 days post-hyposaline challenge. The cDNAs used to construct this library were obtained through subtractive hybridization.

characteristic: description

text: Juvenile

text: E. coli

characteristic: lab-host

text: Gill

text: Mytilus edulis

text: Juvenile mussel 23d hyposaline challenge library

text: Plasmid

text: pGEM


text: University of Maine

text: live

text: Generic

text: Library for ESTs whose expression is down-regulated in juvenile mussels 23 days post-hyposaline challenge. The cDNAs used to construct this library were obtained through subtractive hybridization.

text: Juvenile

text: E. coli

text: Gill

text: Mytilus edulis

characteristic: organism

text: Juvenile mussel 23d hyposaline control library

text: Plasmid

text: pGEM


text: University of Maine

text: live

text: Generic

text: Library for ESTs whose expression is up-regulated in juvenile mussels 48 hours post-hyposaline challenge. The cDNAs used to construct this library were obtained through subtractive hybridization.

text: Juvenile

text: E. coli

text: Gill

text: Mytilus edulis

text: Juvenile mussel 48h hyposaline challenge library

characteristic: title

text: Plasmid

text: pGEM


text: University of Maine

text: live

text: Generic

text: Library for ESTs whose expression is down-regulated in juvenile mussels 48 hours post-hyposaline challenge. The cDNAs used to construct this library were obtained through subtractive hybridization.

text: Juvenile

text: E. coli

text: Gill

text: Mytilus edulis

text: Juvenile mussel 48h hyposaline control library

text: Plasmid

text: pGEM


text: University of Maine

text: live

text: Generic

text: Library for ESTs whose expression is up-regulated in juvenile mussels 9 days post-hyposaline challenge. The cDNAs used to construct this library were obtained through subtractive hybridization.

text: Juvenile

text: E. coli

text: Gill

text: Mytilus edulis

text: Juvenile mussel 9d hyposaline challenge library

text: Plasmid

text: pGEM


text: University of Maine

text: live

text: Generic

text: Library for ESTs whose expression is up-regulated in 1 week-old larval mussels 48 hours post-hyposaline challenge. The cDNAs used to construct this library were obtained through subtractive hybridization.

text: Larval

text: E. coli

text: Mytilus edulis

text: Larval mussel hyposaline challenge library

text: Plasmid

text: pGEM


text: University of Maine

text: live

text: Generic

text: Library for ESTs whose expression is down-regulated in 1 week-old larval mussels 48 hours post-hyposaline challenge. The cDNAs used to construct this library were obtained through subtractive hybridization.

text: Larval

text: E. coli

text: Mytilus edulis

text: Larval mussel hyposaline control library

text: Plasmid

text: pGEM


text: Sri Krishnadevaraya University

text: live

text: Generic

text: Seeds were surface sterilized with 0.1% (w/v) sodium hypo chlorite solution for 5 min, thoroughly rinsed with distilled water and germinated in plastic pots contained soil and sand (2:1) mixture and allowed to grow under natural photoperiod (10-12 hrs, 28 +/- 4oC) in the departmental botanic garden. Seedlings were maintained at 100% soil moisture levels (SMLs) by watering daily. Nineteen day-old plants were subjected to moisture stress by with holding water. Leaf samples collected from unstressed (100% SMLs) and stressed (25% SMLs) plants, flash frozen and stored at -80oC. The frozen samples were used for RNA extraction (LiCl2 method). Poly(A) mRNA was isolated using streptavidin paramagnetic particles (Roche, Germany). First-strand cDNA synthesis was initiated from the enriched mRNA of driver (unstressed) using biotin labeled poly dT and Superscript RNaseH- reverse transcriptase (Gibco-BRL, USA). Subtractive hybridization was performed between biotin labeled driver sscDNA (unstressed) and tester (stressed) mRNA at 65oC for 1 hour. The tester-driver hybrids and excess of the driver were separated from the unhybridized tester mRNA using streptavidin paramagnetic particles. Tester mRNAs that did not hybridize with the driver were used for cDNA synthesis (Stratagene). A primary cDNA library was produced using a lambda ZAP XR cDNA synthesis kit (Stratagene). Randomly selected clones amplified using M13F and M13R-primers, PCR product was purified using PCR purification system (Stratagene) and sequenced from the 3' end in a MJ Research PTC-225 Peltier Thermal Cycler using a ABI PRISM BigDye Terminator Cycle Sequencing Kits with AmpliTaq DNA polymerase (FS enzyme) (Applied Biosystems). Sequences are processed using the VecScreen (http://www.ncbi.nlm.nih.gov/VecScreen.html) to remove vector and cloning oligo sequences and various contaminants, and to trim to a high quality region. Sequences that retained at least 100 bases were submitted to GenBank.

text: 21 day-old seedlings

text: E.coli

text: Carthamus tinctorius

text: EcoRI

text: XhoI

text: Leaf

characteristic: tissue-type

text: Safflower (A-1) drought stressed subtracted cDNA library PTC-225

text: Lambda Uni-ZAP XR


text: Genoscope - Centre National de Sequencage

text: live

text: Generic

text: diploid cell

characteristic: cell type

text: Axenic cultures of pure diploid, highly calcified cells were grown in natural seawater supplemented with modified K/5 nutrients under 80 mol photons/m2/s light on a 14:10 light:dark cycle. Cells were harvested at 8 time points over a 24 hour period be ginning when cell abundance was 200000 cells/ml (mid-exponential growth). Nutrients were not limiting at this point. Equal amounts of total RNA was pooled from each time point. RNA from each time point and from the pooled sample was assessed to be of high quality and not degraded based on analysis on a Agilent Bioanalyzer showing clear distinct 28s and 18s ribosomal RNA bands and spectrophotometric determination of high A260/A280 ratios. RT-PCR using several transcribed genes confirmed the absence of detectable genomic DNA contamination. cDNA preparation was performed by Vertis, Inc. Poly-A RNA was purified from total RNA. cDNA was synthesized according to the Vertis full-length enriched cDNA synthesis protocol using an oligo-dT primer. Adaptors were added to 5 and 3 ends during synthesis. Prior to normalization cDNA was amplified by 19 cycles of PCR using high fidelity DNA polymerase. Normalization was carried out by one cycle of denaturation and reassociation of the cDNA. Re-associated ds-cDNA was separated from ss-cDNA (normalized) by a hydroxylapatite column. The ss-DNA was then further amplified by 9 PCR cycles. cDNAs were subject to limited exonuclease digestion to generate 5 overhangs at both ends. cDNA was size-fractionated by agarose gel electrophoresis. Fractions >0.5 kb were purified and directionally ligated into the EcoR1 and BamH1 sites of the plasmid vector pBS II sk+. The following adaptor sequences remained attached to the 5 and 3 ends of the cDNAs: 5'-end (EcoRI-site) 5- GAATTCCTCTGGACCTTGGCTGTCACTCAGTT-3 3-end (BamHI-site) 5- GGATCCCTTGGCTGTCACTCACTGCGAGTCCGAC-(dT25)-3 Ligations were electroporated into T1 Phage resistant TransforMax EC100-T1R (Epicentre) electrocompetent E.coli cells. The percentage of non-recombinant clones in the libraries was determined by re-ligation of the EcoRI / BamHI digested pBS II sk+ vector in the absence of cDNA and was found to be below 5 %. Sequencing was performed using M13 forward primers by Genoscope.

text: Emiliania huxleyi

characteristic: strain

text: diploid cell library (AAZ0ABA)

text: plasmid

text: pBluescript II SK(+)


characteristic: External Id

text: live

text: Generic

text: haploid cell

text: Axenic cultures of pure haploid, highly motile flagellated cells were grown in natural seawater supplemented with modified K/5 nutrients under 80 mol photons/m2/s light on a 14:10 light:dark cycle. Cells were harvested at 8 time points over a 24 hour period beginning when cell abundance was approximately 200000 cells/ml (mid-exponential growth). Nutrients were not limiting at this point. Equal amounts of total RNA was pooled from each time point. RNA from each time point and from the pooled sample was assessed to be of high quality and not degraded based on analysis on a Agilent Bioanalyzer showing clear distinct 28s and 18s ribosomal RNA bands and spectrophotometric determination of high A260/A280 ratios. RT-PCR using several transcribed genes confirmed the absence of detectable genomic DNA contamination. cDNA preparation was performed by Vertis, Inc. Poly-A RNA was purified from total RNA. cDNA was synthesized according to the Vertis full-length enriched cDNA synthesis protocol using an oligo-dT primer. Adaptors were added to 5 and 3 ends during synthesis Prior to normalization cDNA was amplified by 19 cycles of PCR using high fidelity DNA polymerase. Normalization was carried out by one cycle of denaturation and reassociation of the cDNA. Re-associated ds-cDNA was separated from ss-cDNA (normalized) by a hydroxylapatite column. The ss-DNA was then further amplified by 9 PCR cycles. cDN As were subject to limited exonuclease digestion to generate 5 overhangs at both ends. cDNA was size-fractionated by agarose gel electrophoresis. Fractions >0.5 kb were purified and directionally ligated into the EcoR1 and BamH1 sites of the plasmid vector pBS II sk+. The following adaptor sequences remained attached to the 5 and 3 ends of the cDNAs: 5'-end (EcoRI-site) 5- GAATTCCTCTGGACCTTGGCTGTCACTCAGTT-3 3-end (BamHI-site) 5- GGATCCCTTGGCTGTCACTCACTGCGAGTCCGAC-(dT25)-3 Ligations were electroporated into T1 Phage resistant TransforMax EC100-T1R (Epicentre) electrocompetent E.coli cells. The percentage of non-recombinant clones in the libraries was determined by re-ligation of the EcoRI / BamHI digested pBS II sk+ vector in the absence of cDNA and was found to be below 5 %. Sequencing was performed using M13 forward primers by Genoscope.

text: Emiliania huxleyi

text: haploid cell library (AAZ0AAA)

text: plasmid

text: pBluescript II SK(+)

characteristic: vector


text: Michigan State University

text: live

text: Generic

text: Handpicked type IV glands from leaves of growth chamber grown Solanum pennellii LA0716 plants (25oC for 16/8 h photoperiod). Total RNA was extracted from 2000 handpicked type IV trichomes from the leaf tissue of Solanum pennellii LA0716 plants and used directly for cDNA synthesis using the Clontech Super SMART cDNA synthesis kit according to kit protocols but with modified primers. Primers used in the construction of this library: RT1: 5' -TAGAGGCCGAGGCGGCCGACATGTTTTGTTTTTTTTTCTTTTTTTTTTVN-3'; RT2: 5' CGCAAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3'; 5' PCR primer: 5' -AAGCAGTGGTATCAACGCAGAGT-3'; 3' PCR primer: 5' -TAGAGGCCGAGGCGGCCGAC-3'

text: Solanum pennellii

text: Type IV trichomes

text: LA0716 Type IV trichomes


text: Michigan State University

text: live

text: Generic

text: Handpicked type VI glands from leaves of growth chamber grown Solanum pennellii LA0716 plants (25oC for 16/8 h photoperiod). Total RNA was extracted from 2000 handpicked type VI trichomes from the leaf tissue of Solanum pennellii LA0716 plants and used directly for cDNA synthesis using the Clontech Super SMART cDNA synthesis kit according to kit protocols but with modified primers. Primers used in the construction of this library: RT1: 5' -TAGAGGCCGAGGCGGCCGACATGTTTTGTTTTTTTTTCTTTTTTTTTTVN-3'; RT2: 5' CGCAAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3'; 5' PCR primer: 5' -AAGCAGTGGTATCAACGCAGAGT-3'; 3' PCR primer: 5' -TAGAGGCCGAGGCGGCCGAC-3'

text: Solanum pennellii

text: Type VI trichomes

text: LA0716 Type VI trichomes


text: Michigan State University

text: live

text: Generic

text: Handpicked type I glands from leaves of growth chamber grown Solanum habrochaites LA1777 plants (25oC for 16/8 h photoperiod). Total RNA was extracted from 2000 handpicked type I trichomes from the leaf tissue of Solanum habrochaites LA1777 plants and used directly for cDNA synthesis using the Clontech Super SMART cDNA synthesis kit according to kit protocols but with modified primers. Primers used in the construction of this library: RT1: 5' -TAGAGGCCGAGGCGGCCGACATGTTTTGTTTTTTTTTCTTTTTTTTTTVN-3'; RT2: 5' CGCAAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3'; 5' PCR primer: 5' -AAGCAGTGGTATCAACGCAGAGT-3'; 3' PCR primer: 5' -TAGAGGCCGAGGCGGCCGAC-3'

text: Solanum habrochaites

text: Type I trichomes

text: LA1777 Type I trichomes


text: Michigan State University

text: live

text: Generic

text: Handpicked type IV glands from leaves of growth chamber grown Solanum habrochaites LA1777 plants (25oC for 16/8 h photoperiod). Total RNA was extracted from 2000 handpicked type IV trichomes from the leaf tissue of Solanum habrochaites LA1777 plants and used directly for cDNA synthesis using the Clontech Super SMART cDNA synthesis kit according to kit protocols but with modified primers. Primers used in the construction of this library: RT1: 5' -TAGAGGCCGAGGCGGCCGACATGTTTTGTTTTTTTTTCTTTTTTTTTTVN-3'; RT2: 5' CGCAAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3'; 5' PCR primer: 5' -AAGCAGTGGTATCAACGCAGAGT-3'; 3' PCR primer: 5' -TAGAGGCCGAGGCGGCCGAC-3'

text: Solanum habrochaites

text: Type IV trichomes

text: LA1777 Type IV trichomes


text: Michigan State University

text: live

text: Generic

text: Handpicked type I glands from leaves of growth chamber grown Solanum lycopersicum LA3474 plants (25oC for 16/8 h photoperiod). Total RNA was extracted from 2000 handpicked type I trichomes from the leaf tissue of Solanum lycopersicum LA3475 plants and used directly for cDNA synthesis using the Clontech Super SMART cDNA synthesis kit according to kit protocols but with modified primers. Primers used in the construction of this library: RT1: 5-TAGAGGCCGAGGCGGCCGACATGTTTTGTTTTTTTTTCTTTTTTTTTTVN-3'; RT2: 5' CGCAAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3'; 5' PCR primer: 5' -AAGCAGTGGTATCAACGCAGAGT-3'; 3' PCR primer: 5' -TAGAGGCCGAGGCGGCCGAC-3'

text: Solanum lycopersicum

text: Type I trichomes

text: LA3475 Type I trichomes


text: Michigan State University

text: live

text: Generic

text: Type VII enriched gland fraction from leaves of growth chamber grown Solanum lycopersicum LA3475 plants (25oC for 16/8 h photoperiod). Total RNA was extracted from an enriched sample containing mostly type VII trichomes from the leaf tissue of Solanum lycopersicum LA3475 plants and used directly for cDNA synthesis using the Clontech Super SMART cDNA synthesis kit according to kit protocols but with modified primers. Primers used in the construction of this library: RT1: 5' -TAGAGGCCGAGGCGGCCGACATGTTTTGTTTTTTTTTCTTTTTTTTTTVN-3'; RT2: 5' CGCAAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3'; 5' PCR primer: 5' -AAGCAGTGGTATCAACGCAGAGT-3'; 3' PCR primer: 5' -TAGAGGCCGAGGCGGCCGAC-3'

text: Solanum lycopersicum

text: Type VII trichomes

text: LA3475 Type VII trichomes


text: Michigan State University

text: live

text: Generic

text: Accession PI126449

characteristic: cultivar

text: Glandular trichomes were isolated from leaves of the accession PI126449 using a bead beater, followed by filtration through a series of meshes.

text: Mixed stages

characteristic: dev-stage

text: TJC 121

text: Solanum habrochaites

text: EcoRI

text: EcoRI

text: Glandular Trichome

text: pBluescriptSK-


text: Max Planck Institute for Chemical Ecology

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: 3' RACE of Nicotiana attenuata polyA-transcripts

text: rosette

text: Nicotiana attenuata

text: EcoRV with T-overhangs

text: EcoRV with T-overhangs

text: leaf

text: cDNAs of Nicotiana attenuata leaves 3' RACE

text: plasmid

text: pGEM-TEASY (PROMEGA catalog # A1360)


text: Embrapa Soja

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: root

text: Glycine max

text: root

text: Root from Glycine max stressed by drought


text: AgResearch

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Lolium perenne immature tissue infected with Neotyphodium lolii

text: Lolium perenne/Neotyphodium lolii mixed EST library

text: E-I

text: plasmid


text: AgResearch

text: live

text: Generic

characteristic: description

text: E+I

text: plasmid


text: Temasek Life Sciences Laboratory

text: live

text: Generic

text: 35 dph

text: Spleen

characteristic: organ

text: Lates calcarifer

text: The up-regulation library constructed using SSH technique


text: Temasek Life Sciences Laboratory

text: live

text: Generic

text: 35 dph

text: Spleen

text: Lates calcarifer

characteristic: organism

text: The down-regulation library constructed using SSH technique


text: Iranian Research Institute of plant Protection

text: live

text: Generic

text: cDNA AFLP analysis of acid lime infection by Acid lime witches broom phytoplasma (Candidatus Phytoplasma aurantifolia). The cDNA source is leaf of the infected and healthy acid lime (Citrus aurantifolia). And it was isolated by Invitrogen cDNA synthesis kit.

text: Citrus aurantiifolia/Candidatus Phytoplasma aurantifolia mixed EST library

text: Candidatus Phytoplasma aurantifolia cDNA AFLP

characteristic: title


text: Universite Claude Bernard Lyon 1

text: live

text: Generic

text: isolation_source: poor sandy soil from a Pinus sylvestris forest stand ; country: Belgium:North East Belgium, Campine, Paal, Province of Limburg ; environmental_sample; lat_lon: 51.04 N 5.1 E ; collection_date: 11-Nov-2006 ; collected_by: Marmeisse R, Fraissinet-Tachet L, Lehembre F.

text: uncultured eukaryote

text: uncultured eukaryote from poor sandy soil, Paal


text: Universite Claude Bernard Lyon 1

text: live

text: Generic

text: isolation_source: poor sandy soil from a Pinus sylvestris forest stand; ancient industrial site ; country: Belgium:North East Belgium, Campine, Lommel, Province of Limburg ; environmental_sample; lat_lon: 51.14 N 5.15 E ; collection_date: 11-Nov-2006 ; collected_by: Marmeisse R, Fraissinet-Tachet L, Lehembre F.

text: uncultured eukaryote

text: uncultured eukaryote from poor sandy soil, Lommel


text: National Institute of Vegetable and Tea Science

text: live

text: Generic

text: Torvum Vigor

text: full-length enriched cDNA library constructed using SMART method; mixture of the plants treated with 0.1ppm cadmium chloride for 3 days and non-treated control.

text: vegetative stage, 2 months after germination

text: Solanum torvum

text: leaf

characteristic: tissue-type

text: library TVL


text: live

text: Generic

text: Torvum Vigor

text: Solanum torvum

text: root

text: library TVR


text: Shanghai Ocean University

text: live

text: Generic

text: gill, intestine and liver

text: Ctenopharyngodon idella

text: grass carp gill, intestine and liver


text: Huazhong Agricultural University

text: live

text: Generic

text: Emian 22

text: Using cDNA-SRAP technique, gene expression in fiber of Gossypium hirsutum L. cv. Emian 22 at fiber initiation stage (5d to 20 d post anthesis) was compared with Gossypium barbadense L. cv. Pima3-79.

text: fiber initiation stage(5d to 20d post anthesis)

text: fiber

text: Gossypium hirsutum

text: fiber

text: Gossypium hirsutum cv. Emian 22 at fiber initiation stage 5-20dpa


text: University of California, Irvine

text: live

text: Generic

text: Total RNA was extracted from larvae, pupae, male and female adults. Clontech's SMART RACE cDNA Amplification Kit was used to map 5' termini of specific ribozyme sequences.

text: Anopheles gambiae

text: Mosquito combined tissue gene specific mapping


text: Guiyang Medical College

text: live

text: Generic

text: Adult

text: Taenia solium

text: Taenia solium adult full-length cDNA library

text: pDNR-LIB


text: Universite du Quebec a Montreal

text: live

text: Generic

text: Norstar

text: cDNAs were amplified with a gene specific primer and a universal primer (M13F or M13R) using wheat cDNA libraries. PCR products were cloned in pCRII-TOPO for sequencing.

text: Triticum aestivum

text: Mixed crown, leaf and root

text: Genes amplified from wheat mixed cDNA libraries

text: pCRII-TOPO


text: Universite Claude Bernard Lyon 1

text: live

text: Generic

text: isolation_source: sandy-clay soil from a Picea abies forest ; country: France:North East France, Nievre-Morvan, Breuil Chenue forest ; environmental_sample; lat_lon: 47.18 N 4.4 E ; collection_date: 10-Jul-2007 ; collected_by: Marmeisse R, Debaud JC, Damon C.

text: uncultured eukaryote

text: uncultured eukaryote from sandy-clay soil from a Picea abies forest


text: Forestry and Forest Products Research Institute

text: live

text: Generic

text: Fagus crenata

text: inner bark

text: Fagus crenata inner bark


text: National Institute for Plant Genome Research

text: live

text: Generic

text: PRASAD

text: Suppression subtractive hybridization (SSH) library of 6 hour salt stressed foxtail millet.

text: 21 days old seedlings

text: Setaria italica

text: Salinity stress responsive genes from cv. PRASAD (Setaria italica L.)


text: IPICYT

text: live

text: Generic

text: country: Mexico ; identified_by: M. Fabiola Leon-Galvan.

text: Homo sapiens

text: female

characteristic: sex

text: cervical cancer tissue

text: Homo sapiens cervical cancer tissue


text: live

text: Generic

text: adult

text: Fagus crenata

text: inner bark

text: Fagus crenata inner bark cDNA library


text: Waksman Institute, Rutgers University

text: live

text: Generic

text: Wolffia cDNAs were reversed transcribed from polyA-selected mRNAs. SfiIA and SfiIB linkers were added to the cDNAs via the primers used to synthesize first and second strand cDNAs. The cDNAs were cut with SfiI to generate SfiI sticky ends, and the cDNAs were directionally ligated into SfiIA and SfiIB-cut plasmid vector pDNR-LIB. Recombinant plasmids were transformed into E. coli strain DH5a.

text: Mixed stages

text: E. coli DH5a

characteristic: lab-host

text: Whole animal

text: Wolffia arrhiza

text: SfiI

text: WSSP Wolffia arrhiza cDNA library

text: Plasmid

text: pDNR-LIB


characteristic: External Id

text: Huazhong Agricultural University

text: live

text: Generic

text: Gossypium barbadense L. cv. 3-79 in fiber at fiber initiation stage(5d to 20 d post anthesis) compared with Gossypium hirsutum L. cv. Emian 22.

text: fiber

text: Gossypium barbadense

text: fiber

text: Gossypium barbadense cv. 3-79 at fiber initiation stage 5-20dpa


text: Huazhong Agricultural University

text: live

text: Generic

text: Gossypium barbadense L. cv. 3-79 in fiber at fiber initiation stage.

text: fiber initiation stage

text: fiber

text: Gossypium barbadense

text: fiber

text: Gossypium barbadense cv. 3-79 at fiber initiation stage


text: Huazhong Agricultural University

text: live

text: Generic

text: Emian 22

text: Gossypium hirsutum L. cv. Emian 22 in fiber at fiber initiation stage.

text: fiber initiation stage

text: fiber

text: Gossypium hirsutum

text: fiber

text: Gossypium hirsutum cv. Emian 22 at fiber initiation stage


text: Indain Institute of Integrative Medicine

text: live

text: Generic

text: Vegetative

characteristic: cell type

text: Total RNA extracted from leaf tissue and subsequently constructed into cDNA library using Gigapack Gold (Strata gene) Kit. Clones were screened and the amplified products were sequenced using M-13 primers.

text: Mature plant

text: Withania somnifera

text: Bisexual

text: Leaf

text: Withania somnifera cv. WSR08 leaf cDNA library

text: UNI_ZAP Lambda expression vector

characteristic: vector


text: Paris-Lodron University of Salzburg

text: live

text: Generic

text: Genes that were differentially expressed in the green alga Micrasterias denticulata after cadmium treatment. Suppressive Subtractive Hybridization was used to create cDNA library.

text: Micrasterias denticulata

text: single cell alga

text: suppressive subtractive hybridization plate 4;2

text: pGEM-Teasy Vector


text: Paris-Lodron University of Salzburg

text: live

text: Generic

text: Genes that were differentially expressed in the green alga Micrasterias denticulata after NaCl treatment. Suppressive Subtractive Hybridization was used to create cDNA library.

text: Micrasterias denticulata

text: single cell alga

text: suppressive subtractive hybridization NaCl

text: pGEM-Teasy Vector


text: Paris-Lodron University of Salzburg

text: live

text: Generic

text: cDNA library from sequences amplified by degenerate primer.

text: Micrasterias denticulata

text: single cell alga

text: degenerate primer amplification

text: pGEM-Teasy Vector


text: National Institute of Fruit Tree Science

text: live

text: Generic

text: Housui

characteristic: cultivar

text: Pyrus pyrifolia

text: Pyrus pyrifolia cv. Housui library


text: FIOCRUZ/IOC

characteristic: INSDC center name

text: live

text: Generic

text: Eletrocomp cells (Invitrogen) from E. coli MegaX DH10BTMT1R were transformed with >500 bp cDNAs ligated into pTZ57R/T (Fermentas).

text: Fruits at one, two, and three centimeters before yellowing

characteristic: dev-stage

text: Jatropha curcas

text: Sprague-Dawley

characteristic: strain

text: Seeds

text: Jatropha seeds from fruits at three stages of maturation


text: Shanghai Ocean University

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: pre-metamorphosing

text: Paralichthys olivaceus

text: EST sequence from pre-metamorphosing Oliver flounder


text: University of British Columbia

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Lodgepole pines were provided by Mike Carlson, British Columbia Ministry of Forestry and Range, from his pine breeding program for vigorous growth. The leader, first internode (from the top), second internode, and third internode of a single tree with 6 years of growth were mechanically wounded in Sept. 2008 by making horizontal cuts with a razor blade approx. 1 cm apart and on opposing sides of the full length of each stem section 1, 2, 4, and 8 days respectively prior to harvesting the tissue. To harvest, the stem sections were cut from the tree and the bark manually separated from the xylem, the needles removed, and then the bark and xylem were immediately frozen separately in liquid nitrogen. This is the same tree as used for Pco02-Xylem-wounded-Norm (PCO02). A normalized, full-length enriched, directionally cloned library was prepared with the Creator SMART cDNA Library Construction Kit (Clontech, Mountain View, CA, USA) and the TRIMMER-DIRECT cDNA Normalization Kit (Evrogen, Moscow, Russia). Total RNA (234 micrograms) was extracted from 4.35 g of tissue (approx. equal portions of the four stem bark sections) using the method described in Kolosova, N., et al. (2004) BioTechniques 36(5):1. mRNA (4.2 micrograms) was purified from total RNA (190 micrograms) using the Oligotex mRNA Kit (Qiagen). First strand cDNA was prepared from 480 nanograms of mRNA, SuperScript III reverse transcriptase (Invitrogen), CDS-3M primer (Evrogen), and the SMART IV Oligonucleotide (Clontech). Second strand cDNA was prepared by LD-PCR with Phusion Hot Start DNA Polymerase (Finnzymes, Espoo, Finland). This cDNA was then normalized with duplex-specific nuclease and amplified following the TRIMMER-DIRECT protocol, digested with SfiI, size fractionated, and cDNA larger than approx. 500 bp was ligated into SfiI-digested pDNR-LIB (Clontech). The ligation mixture was transformed into ElectroMAX DH10B T1 Phage-Resistant electro-competent cells (Invitrogen). Titre of the primary library (1 mL) was approx. 1.8x10^6 cfu/mL and was not amplified.

characteristic: description

text: E. coli DH10B

text: Pinus contorta

text: Sfi I

text: Sfi I

text: Bark (phloem and cambium attached) from leader and internodes of a mechanically wounded tree

text: Pco01-Bark-Wounded-Norm (PCO01)

text: Plasmid

text: pDNR-LIB


text: University of British Columbia

text: live

text: Generic

text: Lodgepole pines were provided by Mike Carlson, British Columbia Ministry of Forestry and Range, from his pine breeding program for vigorous growth. The leader, first internode (from the top), second internode, and third internode of a single tree with 6 years of growth were mechanically wounded in Sept. 2008 by making horizontal cuts with a razor blade approx. 1 cm apart and on opposing sides of the full length of each stem section 1, 2, 4, and 8 days respectively prior to harvesting the tissue. To harvest, the stem sections were cut from the tree and the bark manually separated from the xylem, the needles removed, and then the bark and xylem were immediately frozen separately in liquid nitrogen. This is the same tree as used for Pco01-Bark-Wounded-Norm (PCO01). A normalized, full-length enriched, directionally cloned library was prepared with the Creator SMART cDNA Library Construction Kit (Clontech, Mountain View, CA, USA) and the TRIMMER-DIRECT cDNA Normalization Kit (Evrogen, Moscow, Russia). Total RNA (395 micrograms) was extracted from 16.45 g of xylem tissue (approx. equal portions of the four stem sections) using the method described in Kolosova, N., et al. (2004) BioTechniques 36(5):1. mRNA (2.56 micrograms) was purified from total RNA (200 micrograms) using the Oligotex mRNA Kit (Qiagen). First strand cDNA was prepared from 315 nanograms of mRNA, SuperScript III reverse transcriptase (Invitrogen), CDS-3M primer (Evrogen), and the SMART IV Oligonucleotide (Clontech). Second strand cDNA was prepared by LD-PCR with Phusion Hot Start DNA Polymerase (Finnzymes, Espoo, Finland). This cDNA was then normalized with duplex-specific nuclease and amplified following the TRIMMER-DIRECT protocol, digested with SfiI, size fractionated, and cDNA larger than approx. 500 bp was ligated into SfiI-digested pDNR-LIB (Clontech). The ligation mixture was transformed into ElectroMAX DH10B T1 Phage-Resistant electro-competent cells (Invitrogen). Titre of the primary library (1 mL) was approx. 1.1x10^6 cfu/mL and was not amplified.

text: E. coli DH10B

text: Pinus contorta

characteristic: organism

text: Sfi I

text: Sfi I

text: Xylem from leader and internodes of a mechanically wounded tree

text: Pco02-Xylem-wounded-Norm (PCO02)

characteristic: title

text: Plasmid

text: pDNR-LIB


text: School of Life Sciences, Xiamen University

text: live

text: Generic

text: Male Sebastiscus marmoratus weighing 23-27 g, were captured from coastal area in Xiamen city, Fujian province, China. Before the exposure experiment, the fish were acclimated in tanks (25 fish in each tank) containing 50 L of aerated sand-filtered seawater, with natural photoperiod for 7 days. Fish were dosed via single intraperitoneal injection with TBT (0.5 mg/kg body weight in corn oil) or with corn oil as vehicle controls. After injection for 3 days, fish were randomly sampled from each group. The isolated brains were frozen in liquid N2 immediately stored at -80o until analyzed. For the SSH procedure, mRNA was isolated from brains and two libraries constructed in pMD18-T vector, enriched for genes up- (forward subtracted) or down-regulated (reverse subtracted), respectively in response to TBT.

text: Adult

text: E.coli TOP10

text: Brain

characteristic: organ

text: Sebastiscus marmoratus

text: Female

characteristic: sex

text: Suppression subtractive hybridization libraries for brain of Sebastiscus marmoratus in response to TBT

text: Plasmid


characteristic: External Id

text: live

text: Generic

text: Male Sebastiscus marmoratus weighing 23-27 g, were captured from coastal area in Xiamen city, Fujian province, China. Before the exposure experiment, the fish were acclimated in tanks (25 fish in each tank) containing 50 L of aerated sand-filtered seawater, with natural photoperiod for 7 days. Fish were dosed via single intraperitoneal injection with PCBs (3mg/kg body weight, dissolved in corn oil) or with only corn oil as a control. All groups were injected two times, and injections were repeated interval 3 days. After 1 week exposure, fish were randomly sampled from each group. The isolated brains were frozen in liquid N2 and immediately stored at -80o until analysis. For the ACP-ddRT-PCR procedure, 20 arbitrary and 2 dT primers were designed for this study, the total RNA for analysis was extracted from 10 brains homogenate. After ddRT-PCR analyze, the differentially expressed bands were extracted and cloned into pMD18-T vector, enriched for differential display cDNA library in response to PCBs.

text: Adult

text: E.coli TOP10

characteristic: lab-host

text: Brain

text: Sebastiscus marmoratus

text: Female

text: Anneal Control Primer-based Differential Display cDNA library for brain of Sebastiscus marmoratus in response to PCBs

text: Plasmid


text: College of Life Sciences, Wuhan University

text: live

text: Generic

text: petit havana SR1

text: cDNA of the apical and basal cells from tobacco two-celled proembryo was subtracted and cloned into vector for screening, then the identified clones was sequenced for EST analysis.

text: two-celled proembryo

text: E.coli

text: embryo

text: Nicotiana tabacum

text: Hermaphrodite

text: embryo

characteristic: tissue-type

text: two celled proembryo suppression subtractive cDNA library

text: Plasmid

text: pGEMT Easy

characteristic: vector


text: Georg-August University of Goettingen

characteristic: INSDC center name

text: live

text: Generic

text: Total RNA from the nacre forming region of the mantle derived from a rapidly growing mature Haliotis asinina individual was extracted using TriReagent. PolyA mRNA was isolated using Dynabeads (according to the manufactures instructions). A cDNA library was generated using the Cloneminer kit (Invitrogen) and inserts were cloned into the pDONR222 vector and directionally sequenced from the 5' end using standard Sanger termination chemistry.

text: Adult

text: Mantle

text: Haliotis asinina

text: Adult tropical abalone mantle (Haliotis asinina)

text: Plasmid


text: Georg-August University of Goettingen

text: live

text: Generic

text: Total RNA from the nacre forming region of the mantle derived from a rapidly growing mature Pinctada maxima individual was extracted using TriReagent. PolyA mRNA was isolated using Dynabeads (according to the manufactures instructions). A cDNA library was generated using the Cloneminer kit (Invitrogen) and inserts were cloned into the pDONR222 vector and directionally sequenced from the 5' end using standard Sanger termination chemistry.

text: Adult

characteristic: dev-stage

text: Mantle

text: Pinctada maxima

text: Adult silver lipped oyster (Pinctada maxima)

text: Plasmid


text: live

text: Generic

text: The red-magenta fully expanded leaves of E. sagittatum growing in Wuhan Botanical Garden, The Chinese Academy of Sciences, were harvested and prepared for RNA isolation. Sequenced by Sanger method.

text: full-bloom stage

text: Epimedium sagittatum

text: leaf

text: Epimedium sagittatum full-bloom stage leaf EST-SSR


text: Guiyang Medical College

text: live

text: Generic

text: Adult

text: Taenia saginata

text: Taenia saginata adult full-length cDNA library

text: Cosmid

text: PBluescript II SK


text: University of British Columbia

text: live

text: Generic

text: White pine weevil-attacked Sitka spruce (Picea sitchensis) leaders were removed July 23 2007 from the Grafton Road site near Campbell River, BC, Canada (tree GPS N 49 16.771' W 124 29.361') and stored at 4oC. On August 27 2007 all Pissodes strobi (Peck) larvae of mixed instars and undetermined sex were removed from the leader, placed in individual vials, flash frozen with liquid nitrogen and stored at -80oC. A normalized, full-length enriched, directionally cloned library was prepared with the Creator SMART cDNA Library Construction Kit (Clontech, Mountain View, CA, USA) and the TRIMMER-DIRECT cDNA Normalization Kit (Evrogen, Moscow, Russia). Total RNA (256 micrograms) was extracted from frozen whole larvae (weight undetermined) using the Qiagen RNeasy Mini Plant Kit. mRNA (1.3 micrograms) was purified from total RNA (256 micrograms) using the Oligotex mRNA Kit (Qiagen). First strand cDNA was prepared from 403 nanograms of mRNA, SuperScript III reverse transcriptase (Invitrogen), CDS-3M primer (Evrogen), and the SMART IV Oligonucleotide (Clontech). Second strand cDNA was prepared by LD-PCR with Phusion Hot Start DNA Polymerase (Finnzymes, Espoo, Finland). This cDNA was then normalized with duplex-specific nuclease and amplified following the TRIMMER-DIRECT protocol, digested with SfiI, size fractionated, and the cDNA larger than approx. 500 bp was ligated into SfiI-digested pDNR-LIB (Clontech). The ligation mixture was transformed into ElectroMAX DH10B T1 Phage-Resistant electro-competent cells (Invitrogen). Titre of the primary library (2 mL) was approx. 3.2x10^5 cfu/mL and was not amplified.

text: larvae of mixed instars

text: E. coli DH10B

text: Pissodes strobi

text: Sfi I

text: Sfi I

text: undetermined

text: whole larvae

text: Pst02-Larvae-Untreated-Norm (PST02)

text: Plasmid

text: pDNR-LIB


text: University of British Columbia

text: live

text: Generic

text: White pine weevil-attacked Sitka spruce (Picea stichensis) leaders were removed in the fall of 2007 from two British Columbia Forest Service research plantations in the Campbell River area (49o57'N, 125o16'W). Weevils were left to emerge in 16 L ventilated plastic pails placed in a growth chamber (Environette) set to 22oC with an 18:6 light-dark cycle. Every second day emergent adults were removed from the pails and transferred to fresh Sitka spruce cuttings in 2.25 L plastic pails. Emergence continued over a 5 week period. The weevils remained for 5 weeks in the emergence chamber before being transferred to a growth chamber (Conviron) set to a 6oC day and a 4oC night regime. Weevils were fed from 9-year-old Sitka spruce trees located at the University of British Columbia farm (south Point Grey campus). The ends of the food branch sections were immersed in heated paraffin wax to reduce water loss. In addition, moistened Whatman paper was placed in the bottom of each pail and each pail was misted with water every second day. Fresh food was added at 10-14 day intervals. In late June 2008, weevils were sexed and placed in petri dishes lined with moistened paper towel and starved for 48 hours. Weevils were then placed on 4-year old weevil resistant (cultivar 898) and weevil susceptible (cultivar 903) Sitka spruce trees in a mesh cage and allowed to feed upon both as they choose. After 23 days, the weevils (9 male and 11 female) were removed, flash frozen whole in liquid nitrogen, and stored at -80oC. A normalized, full-length enriched, directionally cloned library was prepared with the Creator SMART cDNA Library Construction Kit (Clontech, Mountain View, CA, USA) and the TRIMMER-DIRECT cDNA Normalization Kit (Evrogen, Moscow, Russia). Total RNA (22 and 35 micrograms from males and females, respectively) was extracted from frozen whole adults (weight undetermined) using the Qiagen RNeasy Mini Plant Kit. First strand cDNA was prepared from 695 nanograms of total RNA (equal proportions of male and female RNA), SuperScript III reverse transcriptase (Invitrogen), CDS-3M primer (Evrogen), and the SMART IV Oligonucleotide (Clontech). Second strand cDNA was prepared by LD-PCR with Phusion Hot Start DNA Polymerase (Finnzymes, Espoo, Finland). This cDNA was then normalized with duplex-specific nuclease and amplified following the TRIMMER-DIRECT protocol, digested with SfiI, size fractionated, and the cDNA larger than approx. 500 bp was ligated into SfiI-digested pDNR-LIB (Clontech). The ligation mixture was transformed into ElectroMAX DH10B T1 Phage-Resistant electro-competent cells (Invitrogen). Titre of the primary library (1 mL) was approx. 3.1x10^5 cfu/mL and was not amplified.

text: adult

text: E. coli DH10B

text: Pissodes strobi

text: Sfi I

text: Sfi I

text: male and female

text: whole adult

text: Pst04-Adult-Untreated-Norm (PST04)

text: Plasmid

text: pDNR-LIB


text: Institute of Hydrobiology, Chinese Academy of Sciences

text: live

text: Generic

text: Eriocheir sinensis

text: cDNA library of Eriocheir sinensis


text: Institute of Marine Research

text: live

text: Generic

text: Normalized library

text: 450-850 day degrees

text: Salmo salar

text: notochord

text: Salmon notochord cDNA library from day degrees 450-850

text: Plasmid

text: pDNR-LIB


text: Marine Living Resources Research Department, KORDI

text: live

text: Generic

text: Pagrus major

text: skeletal muscle

text: Pagrus major, skeletal muscle cDNA library


text: live

text: Generic

text: Platichthys stellatus

text: skeletal muscle

text: Platichthys stellatus, skeletal muscle cDNA library


text: Kyushu University

text: live

text: Generic

text: Cyprinus carpio

text: EcoRI

text: XhoI

text: gill

text: Cyprinus carpio gill

text: uniZAP


text: Kyushu University

text: live

text: Generic

text: Cyprinus carpio

text: EcoRI

text: XhoI

text: intestine

text: Cyprinus carpio intestine

text: uniZAP


text: Kansas State University

text: live

text: Generic

text: First instars 0-24 hours old were collected and RNA extracted. Thrips were reared on green bean pods at 23oC.

text: first instar larvae

text: Frankliniella occidentalis

text: Sfi I

text: Hawaiian

characteristic: strain

text: entire bodies

text: First instar Frankliniella occidentalis library

text: plasmid

text: pDNR-LIB vector (Clontech)


text: University of Texas at Austin

characteristic: INSDC first public

text: live

text: Generic

text: This is a directionally cloned, non-normalized library from Ipomopsis aggregata (Polemoniaceae). The library was constructed from rosette leaf material generated from F2 individuals of a cross between subspecies candida and collina. Leaves were in varying stages of development and a variety of stresses (drought stress, low temp, high light) were imposed on plants prior to harvest to create a diverse mRNA pool. This library has been submitted to the Plant Genome Network (PGN). The Plant Genome Network is funded by NSF's Plant Genome Research Program (DBI-0115684). More information about the project can be obtained at http://pgn.cornell.edu.

text: E.coli DH10B

text: Leaf

text: Ipomopsis aggregata

text: EcoRV

text: XhoI

text: rosette leaf

text: Plasmid