text: Kyushu University School of Medicine

text: live

text: Generic

text: Homo sapiens

text: Total human library (David Schlessinger)


status: PUBLIC

characteristic: External Id

text: Kyushu University School of Medicine

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

tag: attribute

characteristic: cell-line

text: Chromosome 7 specific library containing human DNA derived from the somatic cell hybrid cell line 4AF1/106/KO15

characteristic: description

text: Homo sapiens

characteristic: organism

text: 4AF1/106/KO15 library (Lap-Chee Tsui)

characteristic: title


text: The University of York

text: live

text: Generic

text: Total mRNA was isolated from lung stage worms and the cDNA expression library was constructed using the Lambda Zap cDNA cloning system (Stratagene-Uni-Zap XR), the cDNA being directionally cloned between the EcoRI and the XhoI sites of the Bluescript phagemid. Bluescript phagemid was excised by mass in vivo excision of the library and inserted into E.coli XL1 Blue. The library was prepared by Richard Harrop, Department of Biology, University of York, York. YO1 5YW.

text: XL1 Blue

characteristic: lab-host

text: Schistosoma mansoni

text: EcoRI

text: XhoI

text: Puerto Rican

characteristic: strain

text: lung

characteristic: tissue-type

text: SmL

text: Phagemid

text: Bluescript

characteristic: vector


text: University of Florida

text: live

text: Generic

text: mRNA isolated from germinating conidia, grown in 1x Vogel's, 2% sucrose for 4.5 hours

text: germinating conidia

characteristic: dev-stage

text: Neurospora crassa

text: Mating type A

characteristic: sex

text: 74-OR23-IV A

text: Conidia

text: Nelson et al. (1997) conidial library


characteristic: External Id

text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Ductal breast tumor. 5' adaptor sequence: 5' GAATTCGGCACGAG 3' 3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3' Average insert size: 0.9 kb.

text: SOLR (kanamycin resistant)

text: breast

characteristic: organ

text: Homo sapiens

text: EcoRI

text: XhoI

text: female

text: breast tumor

text: phagemid (ampicillin resistant)

text: Bluescript SK-


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: 1st strand cDNA was prepared from RER+ colon tumor, and was then primed with a Not I - oligo(dT) primer. Double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library is not normalized. Library was constructed by Bento Soares and M. Fatima Bonaldo (Soares4).

text: colon

text: Homo sapiens

text: colon tumor RER+

text: phagemid (ampicillin resistant)


characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Pooled colon tumors. 5' adaptor sequence: 5' GAATTCGGCACGAG 3' 3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3' Average insert size: 1.2 kb.

text: SOLR (kanamycin resistant)

text: colon

text: Homo sapiens

text: EcoRI

text: XhoI

text: mixed

text: colon tumor

text: phagemid (ampicillin resistant)

text: Bluescript SK-


text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Cloned unidirectionally. Primer: Oligo dT. Pooled gastric tumors. 5' adaptor sequence: 5' GAATTCGGCACGAG 3' 3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3' Average insert size: 1.0 kb.

text: SOLR (kanamycin resistant)

text: stomach

text: Homo sapiens

text: EcoRI

text: XhoI

text: gastric tumor

text: phagemid (ampicillin resistant)

text: Bluescript SK-


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Mixed germ cell tumors. 5' adaptor sequence: 5' GAATTCGGCACGAG 3' 3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3' Average insert size: 0.7 kb.

characteristic: description

text: SOLR (kanamycin resistant)

text: Homo sapiens

text: EcoRI

text: XhoI

text: germ cell tumor

text: phagemid (ampicillin resistant)

text: Bluescript SK-


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Pooled kidney tumors. 5' adaptor sequence: 5' GAATTCGGCACGAG 3' 3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3' Average insert size: 1.0 kb.

text: SOLR (kanamycin resistant)

text: kidney

text: Homo sapiens

characteristic: organism

text: EcoRI

text: XhoI

text: mixed

text: kidney tumor

text: phagemid (ampicillin resistant)

text: Bluescript SK-


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Larynx. 5' adaptor sequence: 5' GAATTCGGCACGAG 3' 3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3' Average insert size: 0.9 kb.

text: SOLR (kanamycin resistant)

text: larynx

text: Homo sapiens

text: EcoRI

text: XhoI

text: larynx

characteristic: title

text: phagemid (ampicillin resistant)

text: Bluescript SK-


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Pheochromocytoma. 5' adaptor sequence: 5' GAATTCGGCACGAG 3' 3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3' Average insert size: 1.3 kb.

text: SOLR (kanamycin resistant)

characteristic: lab-host

text: Homo sapiens

text: EcoRI

text: XhoI

text: pheochromocytoma

text: phagemid (ampicillin resistant)

text: Bluescript SK-


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Pooled prostate tumors. 5' adaptor sequence: 5' GAATTCGGCACGAG 3' 3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3' Average insert size: 1.2 kb.

text: SOLR (kanamycin resistant)

text: prostate

text: Homo sapiens

text: EcoRI

text: XhoI

text: male, pooled

text: prostate tumor

text: phagemid (ampicillin resistant)

text: Bluescript SK-


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Synovial sarcoma. 5' adaptor sequence: 5' GAATTCGGCACGAG 3' 3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3' Average insert size: 1.4 kb.

text: SOLR (kanamycin resistant)

text: Homo sapiens

text: EcoRI

text: XhoI

text: synovial sarcoma

text: phagemid (ampicillin resistant)

text: Bluescript SK-


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT.

text: Homo sapiens

text: SalI

text: NotI

text: bulk alveolar tumor

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: colon

text: Homo sapiens

text: SalI

text: NotI

text: bulk tumor

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: Homo sapiens

text: SalI

text: NotI

text: bulk germ cell seminoma

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: ovary

text: Homo sapiens

text: SalI

text: NotI

text: female

text: ovary bulk tumor

text: phagemid (ampicillin resistant)


text: Tokai University School of Medicine

text: live

text: Generic

text: mRNA of Entamoeba histolytica HM-1:IMSS strain, trophozoite was prepared with QuickPrep Micro mRNA purification kit (Pharmacia). The first cDNA primed with a NotI-oligo(dT) primer, double-strand cDNA was ligated to EcoRI adapters with TimeSaver cDNA Synthesis Kit and Directional Cloning Toolbox (Pharmacia), and digested with NotI and cloned into the NotI and EcoRI sites of lambdaExCell (Pharmacia). Library was constructed by Tomoo Tanaka.

text: trophozoite

text: Entamoeba histolytica

characteristic: strain

text: Entamoeba histolytica HM-1:IMSS mRNA (Tomoo Tanaka)


text: Smith College Department of Biological Sciences

text: live

text: Generic

text: Brugia malayi is a lymphatic filarial nematode parasite of humans. mRNA was prepared from third stage larvae of Brugia malayi isolated from the peritoneal cavity of jirds nine days after infection. The mRNA was converted to double stranded cDNA using reverse transcriptase and oligo (dT) followed by Rnase H and DNApol I. The library was constructed by Michelle Lizotte-Waniewski. The library is available from the Filarial Genome Project Resource Center: contact Dr. S.A. Williams, Clark Science Center, Smith College, Northampton, MA 01063 USA phone +1 413 585-3826 fax +1 413 585-3786 email genome@smith.edu

text: third stage larvae, nine days after infection

text: E. coli XL1-Blue MRF'

text: Brugia malayi

text: Eco RI (5' end)

text: Xho I (3' end)

text: Brugia malayi L3 molting-day 9 larva cDNA (SAW97MLW-BmL3d9)

text: Phage

text: LambdaZap II (UniZAP XR)


text: Deutsches Krebsforschungszentrum Heidelberg

text: live

text: Generic

text: adult

text: Homo sapiens

text: amygdala

characteristic: tissue-type


text: live

text: Generic

text: adult

text: Homo sapiens

text: subthalamic nucleus


text: live

text: Generic

text: adult

text: Homo sapiens

text: thymus

text: DKFZphthml


text: University of New Mexico

text: live

text: Generic

text: mRNA isolated from germinating conidia, grown in 1x Vogel's, 2% sucrose for 4.5 hours. cDNA directionally cloned into pBluescript SK(-) using the Uni-ZAP XR vector system (Stratagene, La Jolla, CA).

text: germinating conidia

text: Neurospora crassa

text: Mating type A

text: 74-OR23-IV A

text: Conidia

text: Conidial

text: pBluescript SK(-)

characteristic: vector


text: University of New Mexico

text: live

text: Generic

text: mRNA isolated from 5 day old perithecia (fruiting bodies) of the fluffy strain fl a (Mating type a), fertilized with conidia from 74-OR23-IV A (Mating type A). cDNA directionally cloned into pBluescript SK(-) using the Uni-ZAP XR vector system (Stratagene, La Jolla, CA).

text: perithecia

characteristic: dev-stage

text: Neurospora crassa

text: Mating type a (fluffy), fertilized

text: fl a

text: Perithecia (fruiting bodies)

text: Perithecial

text: pBluescript SK(-)


text: Beijing Institute of Radiation Medicine

text: live

text: Generic

text: E.coli JM109

text: Homo sapiens

text: Human embryo lung HEL cell (Pingkun Zhou)

text: Phagemid

text: pGEM-T


text: live

text: Generic

text: mRNA made from normal ovarian cortical stroma, cDNA made by oligo-dT priming. Non-directionally cloned. Size-selected on agarose gel, average insert size 600 bp.

text: ovary

text: Homo sapiens

text: female

characteristic: sex

text: normal cortical stroma

text: plasmid (ampicillin resistant)


characteristic: External Id

text: Washington University School of Medicine

text: live

text: Generic

text: 1st strand cDNA was primed with an oligo(dT) primer. Library was constructed in the Hybri-ZAP vector (Stratagene) using 5' and 3' linker adaptors. Average insert size 1.7 kb. This library was constructed by members of the laboratories of Dr. P. Hackett, Dr. M. Halpern, and Dr. S. Ekker.

text: shield stage (45-55% epiboly) at 6 hrs development

text: XL-1 Blue MRF'/XLOLR

text: Danio rerio

text: EcoRI (5')

text: XhoI (3')

text: embryo

text: Ekkerearly gastrulation zebrafish embryo

text: phagemid (ampicillin resistant)

text: pAD-GAL4 (Stratagene)


text: live

text: Generic

text: 1st strand cDNA was primed with an oligo(dT) primer. Library was constructed in the Hybri-ZAP vector (Stratagene) using 5' and 3' linker adaptors. Average insert size 1.5 kb. This library was constructed by members of the laboratories of Dr. P. Hackett, Dr. M. Halpern, and Dr. S. Ekker.

text: 24 hr development, completion of eye pigment development, but lacking body pigmentation

text: XL-1 Blue MRF'/XLOLR

text: Danio rerio

text: EcoRI (5')

text: XhoI (3')

text: embryo

text: Ekkerpost segmentation zebrafish embryo

text: phagemid (ampicillin resistant)

text: pAD-GAL4 (Stratagene)


text: live

text: Generic

text: 1st strand cDNA was primed with an oligo(dT) primer. Library was constructed in the Hybri-ZAP vector (Stratagene) using 5' and 3' linker adaptors. Average insert size 1.2 kb. This library was constructed by members of the laboratories of Dr. P. Hackett, Dr. M. Halpern, and Dr. S. Ekker.

text: up to 512-cell stage

text: XL-1 Blue MRF'/XLOLR

text: Danio rerio

text: EcoRI (5')

text: XhoI (3')

text: mixed oocytes + embryos

text: Ekker zebrafish maternal

text: phagemid (ampicillin resistant)

text: pAD-GAL4 (Stratagene)


text: Kyungpook National University

text: live

text: Generic

text: Library name stands for ANtheraea Yamamai SilkGland lamda ZAP II (ANYSG ZAPII).

text: final instar larvae

text: XL1 Blue MRF'

text: Antheraea yamamai

text: EcoRI

text: XhoI

text: posterior silkgland

text: ANYSG ZAPII

text: Lambda ZAPII-XR vector


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Pooled lymphomas. 5' adaptor sequence: 5' GAATTCGGCACGAG 3' 3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3' Average insert size: 0.9 kb.

text: SOLR (kanamycin resistant)

text: lymph node

characteristic: organ

text: Homo sapiens

text: EcoRI

text: XhoI

text: lymphoma

text: phagemid (ampicillin resistant)

text: Bluescript SK-


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Invasive prostate tumor cell line (HPV immortalized). 5' adaptor sequence: 5' GAATTCGGCACGAG 3' 3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3' Average insert size: 1.0 kb.

text: SOLR (kanamycin resistant)

text: prostate

text: Homo sapiens

text: EcoRI

text: XhoI

text: invasive tumor (cell line)

text: phagemid (ampicillin resistant)

text: Bluescript SK-


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Normal prostate epithelial cell line (HPV immortalized). 5' adaptor sequence: 5' GAATTCGGCACGAG 3' 3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3' Average insert size: 1.1 kb.

text: SOLR (kanamycin resistant)

text: prostate

text: Homo sapiens

text: EcoRI

text: XhoI

text: epithelium (cell line)

text: phagemid (ampicillin resistant)

text: Bluescript SK-


text: Anna University/National Institutes of Health

characteristic: INSDC center name

text: live

text: Generic

text: The cDNA library of the Wuchereria bancrofti Mf stage was constructed at Smith College, Northampton by Steven A. Williams and Sandra J. Laney. The library was constructed by conventional method using reverse polymerase and oligo dT as primer. Amplified undiluted titer of the library is approximatly 10E+10 pfu per millilitre and 35% of the library is nonrecombinants. The mean insert is size approx 1000 bp. This library is available from: Dr.Steven A williams Dept of Biological Sciences Clark Science Centre Smith College Northampton,MA 01063 Tel: 413 585-3826 Fax: 413 585-3786

text: XL-1 Blue MRF' strain

text: Wuchereria bancrofti

text: EcoRI (5'end)

text: XhoI (3'end)

text: Phage

text: Uni-ZAP XR


text: University of Washington

characteristic: INSDC first public

text: live

text: Generic

text: sessile

text: intestine

text: Boltenia villosa

text: Boltenia villosa Lambda Zap Library

text: Phage

text: Lambda Zap


text: University of Washington

characteristic: INSDC last update

text: live

text: Generic

text: foregut

text: Petromyzon marinus

text: Petromyzon marinus Lambda Zap Library

text: Phage

text: Lambda Zap


text: Japan Science and Technology Corporation (JST)

text: live

characteristic: INSDC status

text: Generic

text: Mus musculus

text: blastocyst

text: Mouse 3.5-dpc blastocyst cDNA


text: Cedars-Sinai Medical Center

text: live

text: Generic

characteristic: NCBI submission model

text: Lung root and neck lymph nodes from Caucasions 26-69 years old, plus liver and brain tissues from spontaneously aborted fetuses at gestational ages of 19-26 weeks. mRNAs from lymph nodes, fetal liver and fetal brain were mixed at equal concentrations. 5 ug of such mixed mRNAs was reverse transcribed with both oligo(dT) and random hexamer to synthesize double stranded cDNA. Blunt-ended ds-cDNAs were then ligated to an EcoR I (Not I) adaptor. Adaptor ligated cDNAs were then amplified by PCR using EN primer (5'> AATTCGCGGCCGCGTCGAC<3'). PCR amplified cDNAs were enriched for those specific for genomic DNAs from 16p13.3 by cDNA selection. Enriched cDNAs, ranging from 0.5-2.0 kb in size, were cloned into pAMP10 vector to construct the regional genomic DNAs specific cDNA library.

text: Homo sapiens

text: Regional genomic DNA specific cDNA library


text: Mayo Medical Center

text: live

text: Generic

characteristic: NCBI submission package

text: metastatic adenocarcinoma

characteristic: cell type

characteristic: cell-line

text: pancreas

text: Homo sapiens

text: female

text: Caucasian

text: subtracted 3' EST library


text: live

text: Generic

text: mRNA made from normal ovarian epithelium, cDNA made by oligo-dT priming. Non-directionally cloned. Size-selected on agarose gel, average insert size 600 bp. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383.

characteristic: description

text: ovary

text: Homo sapiens

characteristic: organism

text: female

text: normal surface epithelium

characteristic: title

text: plasmid (ampicillin resistant)


text: live

text: Generic

text: mRNA made from normal breast ductal tissue, cDNA made by oligo-dT priming. Non-directionally cloned. Size-selected on agarose gel, average insert size 600 bp. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383.

characteristic: lab-host

text: breast

text: Homo sapiens

text: female

text: normal ductal tissue

characteristic: tissue-type

text: plasmid (ampicillin resistant)


text: Institute of Basic Medical sciences, Peking Union Medical College & Chinese Academy of Medical Sciences

text: live

text: Generic

text: Glialblastoma Cell

text: Total RNA was isolated from human glialblastoma cell line BT325. Then Differential Display RT-PCR was conducted between normal and all-trans Retinoic Acid induced cell. Differentially expressed PCR products were cloned and sequenced.

text: E.coli DH5a

text: Brain

text: Homo sapiens

text: Human Glialblastoma Cell

text: Plasmid

text: PCRII, Invitrogen

characteristic: vector


text: Washington University School of Medicine

text: live

text: Generic

text: 1st strand cDNA primed with (GA)10ACTAGTCTCGAG(T)18, followed by second strand synthesis, and ligated to 5' adapter (5')-aattcggcacgag- 3', 3'-gccgtgctc-5'. cDNA was cloned directionally (EcoRI/XhoI) into Stratagene Zap express lambda phage arms. Mass invivo excision done to obtain inserts in pBK-CMV phagemid.

text: E. coli XLOLR

text: Danio rerio

text: EcoRI

text: XhoI

text: mixed male and female

characteristic: sex

text: 3 day fin regenerates

text: zebrafish fin day3 regeneration

text: phagemid

text: pBK-CMV


characteristic: External Id

text: CNRS, INSERM, ULP

text: live

text: Generic

text: Oligo dT cDNA library constructed from mRNA pooled from pooled kidney tissue from 300 adult zebrafish.

text: XLOLR

text: kidney

text: Danio rerio

text: EcoRI

text: XhoI

text: mixed

text: kidney pooled from 300 wild type adults

text: zebrafish gridded kidney

text: pBK-CMV


text: University of Sydney

text: live

text: Generic

text: Sicala V-1

characteristic: cultivar

text: The cDNA library was made from Gossypium hirsutum roots that had been infected with Verticillium dahliae. The organism which is the source of the sequence has not been definitely determined in all cases.

text: 14 day old cotton seedlings, 24 h post-infection with V. dahliae

characteristic: dev-stage

text: Gossypium hirsutum/Verticillium dahliae mixed EST library

text: root

text: Verticillium dahliae-Gossypium hirsutum interaction Lambda Zap Expression Library

text: Phage

text: Lambda ZAP II


text: Stanford University

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: Jemalong

text: cDNA was synthesized from a pooled mRNA prep from elongating root hairs (30% w/w) and 2-3cm root tips (70% w/w). XhoI-oligo-dT linker-primer and EcoRI adaptors were used. cDNAs was cloned unidirectionally into lambda ZAP Express (Stratagene), amplified, and mass-excised into pBK-CMV vector plasmids. More information is available at http://bio-SRL8.stanford.edu.

text: 2-3 day old seedlings

text: Root

characteristic: organ

text: Medicago truncatula

text: EcoRI

text: XhoI

text: Root hairs & tips

text: MtRHE

text: Plasmid

text: pBK-CMV


text: University of Auckland

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: cDNA fragments from mRNA differential display of the pistillata (pi) floral homeotic mutant inserted in either orientation into pGEM-T vector (Promega).

text: Arabidopsis thaliana

text: ecotype Landsberg erecta

characteristic: strain

text: inflorescence apex with young flowers (< ~Stage 9)

text: pi mRNA differential display clones


text: Chinese Academic of Medical Sciences & PUMC

text: live

text: Generic

characteristic: NCBI submission package

text: embryonic

text: E.coli Y1090r-

text: brain

text: Homo sapiens

text: EcoRI

text: EcoRI

text: male

text: brain

text: Human Brain, Clontech

text: phage

text: lambda gt11


text: University of Texas at Austin

text: live

text: Generic

text: lymphoblastoid cell line RECC-UTC4-1

characteristic: cell-line

text: clones identified using RT-PCR differential display

characteristic: description

text: Gallus gallus

characteristic: organism

text: Chicken lymphoblastoid cell line


text: Johns Hopkins School of Medicine

text: live

text: Generic

text: Brain samples from individuals with schizophrenia, bipolar disease, severe depression, and controls are available from the Stanley Neuropathology Consortium

text: brain

text: Homo sapiens

text: frontal cortex

characteristic: tissue-type

text: Human brain frontal cortex

characteristic: title


text: Univ. of Vienna

text: live

text: Generic

text: HaCaT keratinocytes

characteristic: cell type

text: cDNA was obtained by using mRNA differential display technique from HaCaT keratinocytes

text: Homo sapiens

text: Human HaCaT keratinocyte cDNA


characteristic: External Id

text: Yale University School of Medicine

text: live

text: Generic

text: A unidirectional oligo dT-primed cDNA library was constructed in lambda ZAP II. Clones were selected using the criteria of low reactivity with a total cDNA probe.

text: Insect form

text: Trypanosoma brucei rhodesiense

text: Eco RI

text: Xho I

text: YTat 1.1

text: Trypanosoma brucei rhodesiense ZAP II library

text: Phage

text: Lambda ZAP II

characteristic: vector


text: University of Nevada

text: live

text: Generic

text: Six week old

characteristic: dev-stage

text: Mesembryanthemum crystallinum

text: EcoRI

text: XhoI

text: Leaf

text: Ice plant Lambda Uni-Zap XR expression library, 0 hours NaCl treatment

text: Phagemid

text: Lambda Uni-Zap XR, Bluescript SK-


text: Cambridge Institute for Medical Research

characteristic: INSDC center name

text: live

text: Generic

text: Amastigote

text: Leishmania major

text: Friedlin

text: Leishmania major Amastigote Lambda Zap II library

text: Phage

text: Lambda Zap II


text: The Kansai Electric Power Co.

characteristic: INSDC first public

text: live

text: Generic

text: The cDNA clone was isolated from the halotolerant green alga Chlamydomonas W80 by a functional expression screening in E. coli cells. The principle of the screening method was based on the acquirement of stress tolerance of the bacterial cells carrying the cDNA.

text: Chlamydomonas sp. W80

text: Chlamydomonas W80 lambda ZAP II

text: phage

text: lambda ZAP II


text: live

text: Generic

text: Equal amounts of plasmid DNA from three normalized libraries (fetal lung NbHL19W, testis NHT, and B-cell NCI_CGAP_GCB1) were mixed, and ss circles were made in vitro. Following HAP purification, this DNA was used as tracer in a subtractive hybridization reaction. The driver was PCR-amplified cDNAs from pools of 5,000 clones made from the same 3 libraries. The pools consisted of I.M.A.G.E. clones 297480-302087, 682632-687239, 726408-728711, and 729096-731399. Subtraction by Bento Soares and M. Fatima Bonaldo.

characteristic: lab-host

text: pooled

characteristic: organ

text: Homo sapiens

text: Not I

text: Eco RI

text: phagemid (ampicillin resistant)


text: Washington University School of MedicineP

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: 1st strand cDNA was prepared from mammary gland tissue from a lactating female, and was then primed with a Not I - oligo(dT) primer. Double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library is normalized. Library was constructed by Bento Soares and M. Fatima Bonaldo.

text: Mus musculus

text: female (lactating)

characteristic: sex

text: mammary gland

text: phagemid (ampicillin resistant)


text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Cloned unidirectionally. Primer: Oligo dT. 5' adaptor sequence: 5' GAATTCGGCACGAG 3' 3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3' Library constructed by Dr. D. Stainier (University of California, San Francisco).

text: adult

text: SOLR (kanamycin resistant)

text: heart

text: Danio rerio

text: EcoRI

text: XhoI

text: Stainier zebrafish heart

text: phagemid (ampicillin resistant)

text: Bluescript SK-


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer, double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. mRNA source: 2 pooled kidneys. Library went through one round of normalization. Library constructed by Bento Soares and M. Fatima Bonaldo.

characteristic: description

text: kidney

text: Homo sapiens

text: Not I

text: Eco RI

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' AACTGGAAGAATTCGCGGCCGCAATATTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library went through one round of normalization. Library constructed by Bento Soares and M. Fatima Bonaldo.

text: kidney

text: Homo sapiens

characteristic: organism

text: Not I

text: Eco RI

text: 2 pooled tumors (clear cell type)

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Pooled kidney tumors. 5' adaptor sequence: 5' GAATTCGGCACGAG 3' 3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3' Average insert size: 1.3 kb.

text: SOLR (kanamycin resistant)

text: kidney

text: Homo sapiens

text: EcoRI

text: XhoI

text: tumor

characteristic: tissue-type

text: phagemid (ampicillin resistant)

text: Bluescript SK-


text: live

text: Generic

text: mRNA made from infiltrating ductal carcinoma, cDNA made by oligo-dT priming. Non-directionally cloned. Size-selected on agarose gel, average insert size 600 bp.

text: breast

text: Homo sapiens

text: female

text: infiltrating ductal carcinoma

characteristic: title

text: plasmid (ampicillin resistant)


text: live

text: Generic

text: 1st strand cDNA was prepared from 3 pooled germ cell tumors, and was then primed with a Not I - oligo(dT) primer. Double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library is not normalized. Library was constructed by Bento Soares and M. Fatima Bonaldo.

text: Homo sapiens

text: pooled germ cell tumors

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: 1st strand cDNA was prepared from 3 pooled germ cell tumors, and was then primed with a Not I - oligo(dT) primer. Double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library is normalized. Library was constructed by Bento Soares and M. Fatima Bonaldo.

text: Homo sapiens

text: pooled germ cell tumors

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Germinal center B-cells Library constructed by Dr. L. Staudt (NCI). 5' adaptor sequence: 5' GAATTCGGCACGAG 3' 3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3' Average insert size: 1.1 kb.

text: SOLR (kanamycin resistant)

text: tonsil

text: Homo sapiens

text: EcoRI

text: XhoI

text: germinal center B-cells

text: phagemid (ampicillin resistant)

text: Bluescript SK-


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Thymoma. 5' adaptor sequence: 5' GAATTCGGCACGAG 3' 3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3' Average insert size: 1.0 kb.

text: SOLR (kanamycin resistant)

text: thymus

text: Homo sapiens

text: EcoRI

text: XhoI

text: tumor

text: phagemid (ampicillin resistant)

text: Bluescript SK-


text: live

text: Generic

text: mRNA made from normal prostate epithelial cells, cDNA made by oligo-dT priming. Non-directionally cloned. Size-selected on agarose gel, average insert size 600 bp. Library made by D. Krizman, NIH.

text: prostate

text: Homo sapiens

text: male

text: epithelium

text: plasmid (ampicillin resistant)


text: live

text: Generic

text: mRNA made from invasive prostate tumor cells, cDNA made by oligo-dT priming. Non-directionally cloned. Size-selected on agarose gel, average insert size 600 bp. Library made by D. Krizman, NIH.

text: prostate

text: Homo sapiens

text: male

text: tumor

text: plasmid (ampicillin resistant)


text: live

text: Generic

text: mRNA made from prostate BPH, stroma, cDNA made by oligo-dT priming. Non-directionally cloned. Size-selected on agarose gel, average insert size 600 bp. Library made by D. Krizman, NIH.

text: prostate

text: Homo sapiens

text: male

text: stroma

text: plasmid (ampicillin resistant)


text: Lawrence Berkeley National Lab

text: live

text: Generic

text: Constructed using Stratagene ZAP-cDNA Synthesis kit. Oligo dT-primed and directionally cloned at EcoRI and XhoI in BlueScript SK(+/-)

text: adult

text: SOLR

text: head--brain & sensory organ

text: Drosophila melanogaster

text: EcoRI

text: XhoI

text: male and female

text: HL Drosophila melanogaster head BlueScript

text: BlueScript SK


text: Lawrence Berkeley National Lab

text: live

text: Generic

text: Sized fractionated cDNAs were directly ligated into pOT2.

text: adult

text: XL1 Blue

text: head--brain & sensory organ

text: Drosophila melanogaster

text: EcoRI

text: XhoI

text: male and female

text: HL Drosophila melanogaster head pOT2


text: Lawrence Berkeley National Lab

text: live

text: Generic

text: newly eclosed females: germarium-stage 6

text: SOLR

text: ovary

text: Drosophila melanogaster

text: EcoRI

text: XhoI

text: female

text: GM Drosophila melanogaster ovary BlueScript

text: BlueScript SK


text: Lawrence Berkeley National Lab

text: live

text: Generic

text: XL1 Blue

text: ovary

text: Drosophila melanogaster

text: EcoRI

text: XhoI

text: female

text: GM Drosophila melanogaster ovary pOT2


text: Lawrence Berkeley National Lab

text: live

text: Generic

text: 0 to 24 hours mixed stage embryonic

text: SOLR

text: embryo

text: Drosophila melanogaster

text: EcoRI

text: XhoI

text: male and female

text: LD Drosophila melanogaster embryo BlueScript

text: BlueScript SK


text: Lawrence Berkeley National Lab

text: live

text: Generic

text: 0 to 24 hours mixed stage embryonic

text: XL1 Blue

text: embryo

text: Drosophila melanogaster

text: EcoRI

text: XhoI

text: male and female

text: LD Drosophila melanogaster embryo pOT2


text: Institute of Forest Genetics, USDA Forest Service

text: live

text: Generic

text: variety = menziesii; mRNA was isolated from new growth needle tissue and cloned uni-directionally into Uni-ZAP XR with the ZAP-cDNA Synthesis Kit (Stratagene). The phagemid Bluescript SK (-) was excised from the phage vector using the in vivo excision protocol provided by Stratagene. The nucleotide sequence of the 3' end was obtained by automated sequencing at the Recombinant DNA/Protein Resource Facility, Oklahoma State University, Oklahoma, USA.

text: spring growth

text: Pseudotsuga menziesii

text: BamHI

text: EcoRI

text: hermaphrodite

text: needle tissue

text: Douglas-fir (Pm_1000) cDNA library

text: phage

text: Uni-Zap XR Lambda (Stratagene)


text: Institute of Basic Medical sciences, Peking Union Medical College & Chinese Academy of Medical Sciences

text: live

text: Generic

text: cDNAs were prepared by priming of separate reverse transcriptase reaction with a primer GATGCGGCCGCGTCGACCTCANNNNNN on mRNA from human fetal brain, liver and heart. The resultant cDNDs were amplified by polymerase chain reaction with a primer GATGCGGCCGCGTCGACCTCA. cDNAs were pooled after PCR amplification and prior to selection. The cDNA selection was performed essentially as described by M. Lovett (Proc. Natl. Acad. Sci. USA 88: 9628, 1991)

text: 8 Month

text: E.coli DH5a

text: Brain, Liver, Heart

text: Homo sapiens

text: Male

text: Human Fetal Brain+Liver+Heart

text: Plasmid


text: University of Nevada

text: live

text: Generic

text: Six week old

text: Mesembryanthemum crystallinum

text: EcoRI

text: XhoI

text: Leaf, 30 h 0.4M NaCl

text: Ice plant Lambda Uni-Zap XR expression library, 30 hours NaCl treatment

text: Phagemid

text: Lambda Uni-Zap XR, Bluescript SK-


text: Beth Israel Hospital and Harvard Medical School

text: live

text: Generic

text: fetal

text: Mus musculus

text: liver

text: mouse fetal liver


text: Michigan State University

text: live

text: Generic

text: Flower cDNA library, D. Weigel and associates (reference 217). Library data: RNA source: Landsberg erecta inflorescence with predominately younger flower buds. Features: made using Stratagene UniZap kit directionally cloned; 5' at EcoRI 3' at XhoI site in vivo excision converts into pBluescript SK+ expression vector, IPTG inducible. Complexity = 7 aliquots, each plate-amplified once from 800,000 pfu; average size of 10 randomly checked clones was 1.2 kb. cDNAs found in this library include: agamous 1/8,000, pistillata 1/5,000, leafy 1/500,000. When this library is used please reference the ABRC and: Wiegel, D. et al. (1992) Cell 69:843-859.

text: Arabidopsis thaliana

text: EcoRI

text: XhoI

text: var columbia

characteristic: strain

text: pBluescript SK+


characteristic: External Id

text: Cambridge Institute for Medical Research

text: live

text: Generic

text: Promastigote

characteristic: cell type

text: Leishmania major

text: XhoI

text: NotI

text: Leishmania major promastigote full length cDNA library from logarithmic stage (day 7)

text: Phage

text: Lambda Zap II


text: Lawrence Berkeley National Lab

text: live

text: Generic

text: mRNA purified from rough endoplasmic reticulum-bound polysomes was used as a template. cDNA's directionally cloned at HinDIII and PstI in Bluescript SKt. Cloned into HinDIII, but the site was destroyed to add an adapter sequence. So ClaI is now the restriction enzyme at site I of vector. Primers - 5' universal, 3' m13-20 (reverse), 3' T3, 5' T7.

text: 0 to 24 hours old embryo

characteristic: dev-stage

text: XL1 Blue MRF

text: embryo

text: Drosophila melanogaster

text: ClaI

text: PstI

text: male and female

text: CK Drosophila melanogaster embryo BlueScript

text: plasmid

text: Bluescript SK

characteristic: vector


text: Heinrich-Heine-Universitaet

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: leaf cDNA library from green leaves, unidirectionally cloned

text: green

text: leaf

text: Sorghum bicolor

text: Eco RI

text: Xho I

text: cv. TX430

text: Sorghum bicolor cv. TX430 leaf

text: Phage

text: Lambda ZAP II


text: Institute of Forest Genetics

text: live

text: Generic

text: The tissue source for this library is xylem. The xylem tissue was harvested in spring and summer from branches of seed orchard trees which are clones of the same genotype. Branches were 4-6 inches in diameter. The cDNAs were directionally cloned into Lambda Zap and were rescued as a Bluescript derivative in the EcoRI and XhoI sites.

text: Pinus taeda

text: EcoRI

text: XhoI

text: xylem

text: Plasmid

text: lambda ZAP


text: live

text: Generic

text: Promastigote

text: Leishmania major

text: XhoI

text: NotI

text: Leishmania major promastigote full length cDNA library from early logarithmic stage (day 3)

text: Phage

text: Lambda Zap II


text: Faculty of Science, Ain Shams University

text: live

text: Generic

text: Library provided by Dr. Philip LoVerde, SUNY, Buffalo.

text: adult

text: Schistosoma mansoni

text: female

text: Peurtorican

text: S. mansoni female adult Lambda Zap


text: CHU de Poitiers

text: live

text: Generic

text: mRNA was purified from WI-38 cell line, ATCC#CCL 75, 75% exponentially growing (23 passages). cDNA was XhoI-(dT)15 primed. Double-stranded cDNA was ligated to EcoRI adaptors and cloned in pGAD GL. Before sequencing the insert was excised with EcoRI and XhoI and cloned into pGEM-4Z digested by EcoRI and SalI.

text: Homo sapiens

text: EcoRI

text: XhoI

text: Human Lung cDNA Library

text: plasmid

text: pGAD GL


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: 1st strand cDNA was prepared from neuroendocrine lung carcinoid, and was then primed with a Not I - oligo(dT) primer. Double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library is normalized. Library was constructed by Bento Soares and M. Fatima Bonaldo.

characteristic: lab-host

text: lung

characteristic: organ

text: Homo sapiens

text: carcinoid

text: phagemid (ampicillin resistant)


characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 0.8 kb.

text: liver

text: Homo sapiens

text: SalI

text: NotI

text: hepatic adenoma

text: phagemid (ampicillin resistant)


text: live

text: Generic

characteristic: NCBI submission model

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.3 kb.

text: lung

text: Homo sapiens

text: SalI

text: NotI

text: small cell carcinoma

text: phagemid (ampicillin resistant)


text: live

text: Generic

characteristic: NCBI submission package

text: 1st strand cDNA was prepared from colon adenocarcinoma, and was then primed with a Not I - oligo(dT) primer. Double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library is normalized. Library was constructed by Bento Soares and M. Fatima Bonaldo.

text: colon

text: Homo sapiens

text: adenocarcinoma

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.0 kb.

characteristic: description

text: brain

text: Homo sapiens

text: SalI

text: NotI

text: substantia nigra

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.1 kb.

text: peripheral nervous system

text: Homo sapiens

characteristic: organism

text: SalI

text: NotI

text: dorsal root ganglion

text: phagemid (ampicillin resistant)


text: Theodor Bilharz Research Institute

text: live

text: Generic

text: Poly adenylated RNA was isolated from adult of S. mansoni worms

text: Adult

text: Mus musculus

text: Schistosoma mansoni

text: EcoRI

text: XhoI

text: Mixed

characteristic: sex

text: Egyptian

text: Schistosoma Lambda Zap II

text: Lambda Zap II


text: Advanced Center for Genome Technology, University of Oklahoma

text: live

text: Generic

text: 5' end of cDNA cloned into EcoRI site of pBluescript 3' end of cDNA cloned into XhoI site of pBluescript

text: Aspergillus nidulans

text: EcoRI

text: XhoI

text: FGSC A26

text: vegetative mycelia, asexual structures

characteristic: tissue-type

text: Aspergillus nidulans 24hr asexual developmental and vegetative cDNA lambda zap library

characteristic: title

text: phagemid

text: pBlueScript SK-


characteristic: External Id

text: Mott Center for Human Growth and Development, Wayne State University School of Medicine

text: live

text: Generic

text: Differential display product obtained by using anchored oligo dT primer: deoxyTTTTTTTTTTTTVC (V: Equimolar mixture of dA, dC and dG) and an arbitrary primer: 5'TACAACGAGG3'

text: blastocyst

text: Mus musculus

text: Mouse blastocyst differential display product/primer TACAACGAGG

text: pCR II vector (Invitrogen)


text: The Institute for Genomic Research

text: live

text: Generic

text: lung

text: Rattus sp.

text: EcoRI

text: NotI

text: Normalized rat lung, Bento Soares


text: The Institute for Genomic Research

text: live

text: Generic

text: heart

text: Rattus sp.

text: EcoRI

text: NotI

text: Normalized rat heart, Bento Soares


text: The Institute for Genomic Research

text: live

text: Generic

text: liver

text: Rattus sp.

text: EcoRI

text: NotI

text: Normalized rat liver, Bento Soares


text: The Institute for Genomic Research

text: live

text: Generic

text: ovary

text: Rattus sp.

text: EcoRI

text: NotI

text: Normalized rat ovary, Bento Soares


text: The Institute for Genomic Research

text: live

text: Generic

text: muscle

text: Rattus sp.

text: EcoRI

text: NotI

text: Normalized rat muscle, Bento Soares


text: The Institute for Genomic Research

text: live

text: Generic

text: spleen

text: Rattus sp.

text: EcoRI

text: NotI

text: Normalized rat spleen, Bento Soares


text: The Institute for Genomic Research

text: live

text: Generic

text: placenta

text: Rattus sp.

text: EcoRI

text: NotI

text: Normalized rat placenta, Bento Soares


text: Children's Memorial Research Center

text: live

text: Generic

text: This library consists of a mixture of individually tagged normalized libraries constructed from rat placenta, adult lung, brain, liver, kidney, heart, spleen, ovary, and muscle. The tag is a string of 3-5 nucleotides present between the Not I site and the oligo-dT track which allows identification of the library of origin of a clone within the mixture.

text: adult

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

characteristic: strain

text: Phagemid


text: Chinese Academic of Medical Sciences & PUMC

text: live

text: Generic

text: 6 weeks

characteristic: dev-stage

text: XL1-Blue MRF'

text: Rattus norvegicus

text: EcoR I

text: Xho I

text: Male

text: Sprague-Dawley

text: Brain

text: Rat Brain, Stratagene (cat.#936501)

text: Phage

text: Lambda Zap II


text: National Institute of Diabetes, Digestive and Kidney Diseases

text: live

text: Generic

text: The library was constructed with cDNAs prepared from mouse mammary tissue at day 10 of pregnancy, and subtracted with cDNA from the same stage from Stat5a-null mice. First strand cDNA synthesis was primed with oligo dT. A PCR-Select cDNA substraction kit (Clontech) was used to prepare double stranded cDNAs. Additional information about this library and the expression pattern of individual genes can be retrieved (http://mammary.nih.gov/mgap/libraries/mgap002.html)

text: day 10 of pregnancy

text: mammary gland

text: Mus musculus

text: female

text: mammary


text: Institute of Plant Genetics and Crop Plant Research

text: live

text: Generic

characteristic: cultivar

text: Leaf

text: Solanum lycopersicum

text: whole leaf

text: Tomato Leaf cDNA from cv. LA0490

text: plasmid

characteristic: vector


characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: VFNT cherry

text: Leaf

text: Solanum lycopersicum

text: epidermis

text: Tomato Leaf cDNA from cv. VFNT cherry

text: plasmid

text: pBluescript


text: Washington University School of MedicineP

text: live

text: Generic

text: 1st strand cDNA was prepared from pregnant mouse uterus, and was then primed with a Not I - oligo(dT) primer. Double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library is normalized. Library was constructed by Bento Soares and M. Fatima Bonaldo.

text: adult

characteristic: lab-host

text: uterus

characteristic: organ

text: Mus musculus

text: female

text: phagemid (ampicillin resistant)


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACGAATCTGAAGTGGGAGCGGCCGCCCTTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Eco RI adaptors [AATTCGGATCCTTG], digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library constructed by Bob Barstead.

text: adult, 34 years

text: pancreas

text: Homo sapiens

text: EcoRI

text: NotI

text: female

text: Barstead pancreas HPLRB1

text: phagemid (ampicillin resistant)


text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: adult, 17 years

text: spleen

text: Homo sapiens

text: EcoRI

text: NotI

text: male

text: Barstead spleen HPLRB2

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.3 kb. 5' adaptor sequence: 5' GAATTCGGCACGAG 3' 3' adaptor sequence: 5' (GA)10ACTAGTCTCGAGTTTTTTTTTTTTTTTTTT 3'

characteristic: description

text: SOLR (kanamycin resistant)

text: lymph node

text: Homo sapiens

characteristic: organism

text: EcoRI

text: XhoI

text: squamous cell carcinoma

text: phagemid (ampicillin resistant)

text: Bluescript SK-


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.2 kb. 5' adaptor sequence: 5' GAATTCGGCACGAG 3' 3' adaptor sequence: 5' (GA)10ACTAGTCTCGAGTTTTTTTTTTTTTTTTTT 3'

text: SOLR (kanamycin resistant)

text: larynx

text: Homo sapiens

text: EcoRI

text: XhoI

text: squamous cell carcinoma from vocal cord

characteristic: tissue-type

text: phagemid (ampicillin resistant)

text: Bluescript SK-


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.0 kb. 5' adaptor sequence: 5' GAATTCGGCACGAG 3' 3' adaptor sequence: 5' (GA)10ACTAGTCTCGAGTTTTTTTTTTTTTTTTTT 3'

text: SOLR (kanamycin resistant)

text: tongue

text: Homo sapiens

text: EcoRI

text: XhoI

text: squamous cell carcinoma from base of tongue

characteristic: title

text: phagemid (ampicillin resistant)

text: Bluescript SK-


characteristic: External Id

text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.5 kb. 5' adaptor sequence: 5' GAATTCGGCACGAG 3' 3' adaptor sequence: 5' (GA)10ACTAGTCTCGAGTTTTTTTTTTTTTTTTTT 3'

text: SOLR (kanamycin resistant)

text: pharynx

text: Homo sapiens

text: EcoRI

text: XhoI

text: squamous cell carcinoma

text: phagemid (ampicillin resistant)

text: Bluescript SK-


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.1 kb. Life Technologies catalog #: 10982-015

text: ovary

text: Homo sapiens

text: SalI

text: NotI

text: serous adenocarcinoma

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 0.3 kb. Life Technologies catalog #: 10983-013

text: ovary

text: Homo sapiens

text: SalI

text: NotI

text: poorly differentiated adenocarcinoma

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 0.6 kb. Life Technologies catalog #: 10983-013

text: ovary

text: Homo sapiens

text: SalI

text: NotI

text: well differentiated papillary adenocarcinoma

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Life Technologies catalog #: 10980-019

text: bowel

text: Homo sapiens

text: SalI

text: NotI

text: metastatic melanoma

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 0.9 kb. Life Technologies catalog #: 10981-017

text: bowel (skin primary)

text: Homo sapiens

text: SalI

text: NotI

text: metastatic melanoma to bowel

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.2 kb. Life Technologies catalog #:10985-018

text: breast

text: Homo sapiens

text: SalI

text: NotI

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Life Technologies catalog #: 10989-010

text: liver

text: Homo sapiens

text: SalI

text: NotI

text: hepatoid gastric carcinoma (metastatic to liver)

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Life Technologies catalog #: 10992-014

text: lung

text: Homo sapiens

text: SalI

text: NotI

text: adenocarcinoma

text: phagemid (ampicillin resistant)


text: Yale University School of Medicine

text: live

text: Generic

text: isolated by cDNA hybrid selection using a collection of genomic fragments that bind to the zinc finger oncoprotein EVI-1

text: brain

text: Mus musculus

text: Mouse brain candidate EVI-1 target genes


text: Harvard Medical School

text: live

text: Generic

text: cDNA isolated in a screen for mitotic phosphoproteins; Sequences have not been edited or confirmed by sequencing the complementary strand

text: Xenopus laevis

text: Xenopus laevis mitotic phosphoprotein cDNA


text: Michigan Technological University

text: live

text: Generic

text: mycelium

text: Laccaria bicolor

characteristic: strain

text: Laccaria bicolor mRNA


text: Sogang University

text: live

text: Generic

text: Happy Dry

text: Length of 0.5 to 2 centimeter

characteristic: dev-stage

text: XL1 Blue MRF'

text: leaf

text: Capsicum annuum

text: XhoI

text: EcoRI

text: hermaphrodite

characteristic: sex

text: Leaf cDNA library of Hot pepper

text: plasmid

text: pBluescript SK(-)


text: Sogang University

text: live

text: Generic

text: Happy Dry

characteristic: cultivar

text: Oligo(dT) primed cDNA from poly(A) RNA. In vivo excised to pBluescript plasmid.

text: before anthesis : non-pigmented anther

text: Anther

text: Capsicum annuum

text: EcoRI

text: EcoRI

text: hermaphrodite

text: Anther cDNA library of Hot pepper

text: phage

text: Lambda ZAP II

characteristic: vector


text: Sogang University

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: Happy Dry

text: green fruit with 0.5 to 2 centimeter in length

text: Fruit

text: Capsicum annuum

text: EcoRI

text: EcoRI

text: hermaphrodite

text: Fruit cDNA library of Hot pepper

text: phage

text: Lambda ZAP II


text: Sogang University

text: live

text: Generic

text: Happy Dry

text: floral bud

characteristic: organ

text: Capsicum annuum

text: XhoI

text: EcoRI

text: hermaphrodite

text: Floral bud cDNA library of Hot pepper

text: phage

text: Lambda ZAP II


text: Japan Science and Technology Corporation (JST)

characteristic: INSDC last update

text: live

text: Generic

text: fertilized one-cell-embryo

text: Mus musculus

text: Mouse fertilized one-cell-embryo cDNA


text: University of Freiburg

text: live

characteristic: INSDC status

text: Generic

text: protonema

characteristic: cell type

text: Physcomitrium patens

text: Physcomitrella patens mRNA


text: The Institute for Genomic Research

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: kidney

text: Rattus sp.

text: EcoRI

text: NotI

text: Normalized rat kidney, Bento Soares


text: Kyushu University

text: live

text: Generic

text: peritoneal exudate cells

text: Cyprinus carpio

characteristic: organism

text: Carp alginate-induced 48 hr cDNA


text: University of Wuerzburg

text: live

text: Generic

characteristic: cell-line

text: Homo sapiens

text: retina

characteristic: tissue-type

text: Human retina cell line ARPE-19


characteristic: External Id

text: National Human Genome Research Institute

text: live

text: Generic

text: Trabecular Bone Cells

text: Library constructed by Dr. Marian Young and Dr. Pamela Gehron Robey (NIDCR)

characteristic: description

text: SURE

characteristic: lab-host

text: Hip

text: Homo sapiens

text: EcoRI

text: Female

text: Bone

text: Normal Human Trabecular Bone Cells

characteristic: title

text: Plasmid

text: pBluescript


text: University of Oulu

text: live

text: Generic

text: Produced by differential display using primers CA (TTTTTTTTTTTTCA) and OPA16 (AGCCAGCGAA). Clone is upregulated by DFMO-treatment.

text: Rattus norvegicus

text: EcoRI

text: Rat-2 cells, DFMO-treated (90 min)

text: pCR 2.1


text: University of Oulu

text: live

text: Generic

text: Rattus norvegicus

text: EcoRI

text: Rat-2 cells, DFMO-treated (48h)

text: pCR 2.1


text: Children's Memorial Research Center

text: live

text: Generic

text: This library consists of a mixture of individually tagged normalized libraries constructed from 8, 12 and 18-day embryo. The tag is a string of 3-5 nucleotides present between the Not I site and the oligo-dT track which allows identification of the library of origin of a clone within the mixture.

text: embryonic

characteristic: dev-stage

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: NotI

text: EcoRI

text: Sprague-Dawley

characteristic: strain

text: Phagemid


text: Theodor Bilharz Research Institute

characteristic: INSDC center name

text: live

text: Generic

text: Poly adenylated RNA was isolated from S. mansoni cercariae, and processed for the construction of cDNA expression library in lambda ZAP II vector. Clones were in-vivo excised and dsDNA was sequenced from the 5' end to generate ESTs

text: cercariae

text: Mus musculus

text: Schistosoma mansoni

text: EcoRI

text: XhoI

text: mixed

characteristic: sex

text: Egyptian

text: Schistosoma Lambda Zap II cercariae

text: Lambda Zap II

characteristic: vector


text: University of Leeds

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: isolate=Gransden; cDNA from abscisic acid-treated protonemata

text: Physcomitrium patens

text: protonemata

text: Abscisic acid-treated protonemata

text: phage

text: lambda ZAPII


text: University of Edinburgh

text: live

characteristic: INSDC status

text: Generic

text: adult

text: Ciona intestinalis

text: whole animal, except mantle

text: Ciona intestinalis whole animal

text: phagemid

text: pBluescript SK


text: Tokai University School of Medicine

text: live

text: Generic

characteristic: NCBI submission model

text: 20-year-old adult

text: Homo sapiens

text: caudate

text: Clontech catalog #CLHL1076b


text: Baylor College of Medicine

text: live

text: Generic

characteristic: NCBI submission package

text: fetus (17-18 wk)

text: Homo sapiens

text: female

text: brain

text: Stratagene catalog #936206


text: GlaxoWellcome

text: live

text: Generic

text: adult

text: brain

characteristic: organ

text: Rattus norvegicus

characteristic: organism

text: male

text: Sprague-Dawley

text: Rat brain (C.Patten)


text: University of Utah

text: live

text: Generic

text: Homo sapiens

text: Human (J. Swensen)


text: NC State University

text: live

text: Generic

text: CD-1 mice; caesarean derived

text: Mus musculus

text: Swiss Webster

text: Swiss Webster mouse (S.Branch)


text: Coloproctology Center, Takano Hospital

text: live

text: Generic

text: Oncorhynchus mykiss

text: Rainbow trout cDNA


text: National Institute of Agrobiologocal Resources

text: live

text: Generic

text: Oryza sativa

text: seed

characteristic: tissue-type

text: Rice seed mRNA (A.Suzuki)


characteristic: External Id

text: Instituto de Investigaciones Biotecnologicas (Univ. Nac. de Gral San Martin)

text: live

text: Generic

text: epimastigote

characteristic: cell type

text: cDNA library constructed with oligo dT primed epimastigote mRNA and cloned in pt7t318D phagemid with modified polylinker (PHARMACIA)

text: Trypanosoma cruzi

text: Cl-Brenner

text: T. cruzi epimastigote normalized cDNA Library


text: Washington University School of MedicineP

text: live

text: Generic

text: C2C12 (undifferentiated)

characteristic: cell-line

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACGAATCTGAAGTGGGAGCGGCCGCCCTTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Eco RI adaptors [AATTCGGATCCTTG], digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Source undifferentiated tissue culture cell line C2C12. Library constructed by Bob Barstead. The C2C12 cell line (available from ATCC, catalog # CRL-1772) differentiates rapidly, forming contractile myotubes and producing characteristic muscle proteins.

characteristic: description

characteristic: lab-host

text: Mus musculus

text: EcoRI

text: NotI

text: Barstead stromal cell line MPLRB8

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACGAATCTGAAGTGGGAGCGGCCGCCCTTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Eco RI adaptors [AATTCGGATCCTTG], digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Source irradiated bowel harvested 72 hours after irradiation (1400 Gys). Library constructed by Bob Barstead.

text: 8 weeks

characteristic: dev-stage

text: Mus musculus

text: EcoRI

text: NotI

text: FVB/N

characteristic: strain

text: bowel

text: Barstead bowel MPLRB9

characteristic: title

text: phagemid (ampicillin resistant)


text: Sidney Kimmel Cancer Center

characteristic: INSDC center name

text: live

text: Generic

text: Trypanosoma brucei

text: Differentially expressed Trypanosoma brucei mRNAs


characteristic: INSDC first public

text: live

text: Generic

text: Homo sapiens

text: pancreatic islet

text: Human pancreatic islet cell


text: Ludwig Institute of Cancer Research

characteristic: INSDC last update

text: live

text: Generic

text: peritoneal macrophages

text: obtained from DDRT-PCR analysis on Trypanosoma cruzi's GPI-mucins and/or IFN-gamma induced macrophages

text: Mus musculus

text: Mouse peritoneal macrophages


text: University of Tokyo

text: live

characteristic: INSDC status

text: Generic

text: Homo sapiens

text: EST from 8p21.3-p22


text: Cardiovascular Institute & Fu Wai Hospital

text: live

text: Generic

characteristic: NCBI submission model

text: vascular smooth muscle

text: adult

text: Oryctolagus cuniculus

text: male

characteristic: sex

text: rabbit

text: aorta

text: Rabbit adult aorta


text: Beijing Medical University

text: live

text: Generic

characteristic: NCBI submission package

text: vascular smooth muscle cells

text: product of differential display performed on RNA isolated from cells treated with 10 mM homocysteine for 12 hours

text: Rattus norvegicus

text: vascular smooth muscle

text: Rat vascular smooth muscle cells treated with homocysteine


text: VA Medical Center

text: live

text: Generic

text: 14.5 day gestational embryo

text: Mus musculus

characteristic: organism

text: heart

text: Mouse heart ESTs (Cai,T.)


text: Cancer Institute

text: live

text: Generic

text: retinoic acid-treated

text: Homo sapiens

text: lung adenocarcinoma

characteristic: tissue-type

text: Human lung adenocarcinoma (M.Wu)


characteristic: External Id

text: National Cancer Institute

text: live

text: Generic

text: Homo sapiens

text: brain

text: Human brain (K.Lueders)


text: Shemyakin Institute of Bioorganic Chemistry

text: live

text: Generic

text: Homo sapiens

text: whole cerebral brain

text: Clontech #HL1181a


text: Institute of Basic Medical sciences, Peking Union Medical College & Chinese Academy of Medical Sciences

text: live

text: Generic

text: fetal

text: Homo sapiens

text: brain

text: Clontech Hl1149x


text: International Institute of Genetics and Biophysics

text: live

text: Generic

text: Cyprinus carpio

text: liver

text: carp liver differential display RT-PCR


text: University of Illinois

text: live

text: Generic

text: Bos taurus

text: ovary

text: Bovine ovary cDNA


text: Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais

text: live

text: Generic

text: fibroblast

characteristic: cell type

text: primary cells spontaneously immortalized after TNF alpha induction; IRF-1 deficient

text: embryonic

text: Mus musculus

text: Mouse embryonic fibroblast (E.G.Pires)


text: Baylor College of Medicine

text: live

text: Generic

text: stromal

characteristic: cell-line

text: Mus musculus

text: bone marrow

text: Mouse cell line BMS2 (R.Jurecic)


text: Baylor College of Medicine

text: live

text: Generic

text: hematopoietic

text: Mus musculus

text: Mouse cell line A20 (R.Jurecic)


text: Baylor College of Medicine

text: live

text: Generic

text: hematopoietic

text: Mus musculus

text: Mouse cell line Ba/F3 (R.Jurecic)


text: Baylor College of Medicine

text: live

text: Generic

text: hematopoietic

text: Mus musculus

text: Mouse cell line LyD9 (R.Jurecic)


text: Baylor College of Medicine

text: live

text: Generic

text: hematopoietic

text: Mus musculus

text: Mouse cell line FDC-P1 (R.Jurecic)


text: Baylor College of Medicine

text: live

text: Generic

text: hematopoietic

text: Mus musculus

text: Mouse cell line WEHI-3B (R.Jurecic)


text: Baylor College of Medicine

text: live

text: Generic

text: ES cells

text: Mus musculus

text: Mouse ES cells (R.Jurecic)


text: Baylor College of Medicine

text: live

text: Generic

text: fibroblasts

text: STO

text: embryo

text: Mus musculus

text: Mouse cell line STO (R.Jurecic)


text: USC

text: live

text: Generic

text: HeLa

text: Homo sapiens

text: Human HeLa (Y.Wang)


text: UCSF Medical Center

text: live

text: Generic

text: Mus musculus

text: Differentially displayed mouse mRNA


text: Institute of General Genetics

text: live

text: Generic

text: Homo sapiens

text: Human chromosome 13q14 cDNA


text: Michigan State University

text: live

text: Generic

text: GIFU

characteristic: cultivar

text: Lotus japonicus

text: root nodule

text: Late nodulin expressed sequence tag library


text: Institute of Food Research

text: live

text: Generic

text: Sus scrofa

text: Pig expressed sequence tags


text: Institute of Radiation Medicine

text: live

text: Generic

text: fibroblast

text: cells immortalized by ionizing radiation

characteristic: description

text: fetus

text: Homo sapiens

text: trachea

text: Human fetal trachea fibroblast


text: University of Toronto

text: live

text: Generic

text: Homo sapiens

text: brain

text: Human brain cDNA (Life Technologies)


text: Beijing Institute of Radiation Medicine

text: live

text: Generic

text: RTE

text: Rattus norvegicus

text: Tracheal epithelial transforming EST


text: University of Arizona

text: live

text: Generic

text: 6 hour salt stress

text: 6 week old

characteristic: dev-stage

text: Mesembryanthemum crystallinum

text: root

text: Common ice plant root, 6 hour salt stress


text: Lombardi Cancer Center

text: live

text: Generic

text: Homo sapiens

text: head and neck squamous cell carcinoma

text: Human cell line PCI-O6A


text: Lombardi Cancer Center

text: live

text: Generic

text: Homo sapiens

text: head and neck squamous cell carcinoma

text: Human cell line PCI-O6B


text: SPIC Science Foundation

text: live

text: Generic

text: Pokkali; Vytilla 1

text: 15 day old hydroponically grown rice seedlings were stressed by the addition of NaCl to 1% for 2 days. Total RNA was extracted from the plant tissues, polyA RNA was purified and the cDNA library constructed following the instructor's manual in the Superscript reverse transcriptase system for cDNA synthesis (GIBCO-BRL)

text: 17 day old plants

text: Escherichia coli DH5-alpha

characteristic: lab-host

text: Oryza sativa Indica Group

text: NotI

text: SalI

text: Whole plant

text: Pokkali Rice salt stressed cDNA library

text: phagemid

text: pSPORT1 (GIBCO-BRL)

characteristic: vector


text: Universitaet Tuebingen

characteristic: INSDC center name

text: live

text: Generic

text: Picea abies

text: Norway spruce mRNA

characteristic: title


text: INRA

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: granulosa

text: primary culture; from mRNA Differential Display

text: immature

text: Sus scrofa

text: female

text: ovary

text: Pig ovarian granulosa cell mRNA

text: plasmid


text: Guys Hospital

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Homo sapiens

text: Human mRNA (Tripodis and Ragoussis)


text: National Institute of Environmental Health Sciences

text: live

text: Generic

characteristic: NCBI submission package

text: Homo sapiens

text: fibrosarcoma

text: Human fibrosarcoma cell line HT1080


text: live

text: Generic

text: Homo sapiens

characteristic: organism

text: fibrosarcoma

text: Human fibrosarcoma cell line HT1080-6TGc5


text: The Institute of Medical Science, University of Tokyo

text: live

text: Generic

text: neuroblastoma

text: SK-N-MC

text: 5'-end-enriched cDNA library

text: Homo sapiens

text: Human cell line SK-N-MC


text: Gunma University School of Medicine

text: live

text: Generic

text: Rattus norvegicus

text: Wistar

characteristic: strain

text: ventral medullary surface

characteristic: tissue-type

text: Rat ventral medullary surface


characteristic: External Id

text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: Equal amounts of plasmid DNA from five normalized libraries were mixed, and ss circles were made in vitro. Following HAP purification, this DNA was used as tracer in a subtractive hybridization reaction. The driver was PCR-amplified cDNAs from pools of 5,000 clones made from the same 5 libraries. The pools consisted of the following libraries and cloneIDs: Soares NbHSF pool 1: 309384-310919, 323208-325895 Soares Nb2HP pool 1: 145032-147335, 147720-148103, 148872-149255, 15002 - 150407, 151176-152327 Soares Nb2HF8-9W pool 1: 758280-760583, 772104-774407 Soares NbHPA pool 1: 304776-306311, 320136-322823, 326280-326663 Soares NbHOT pool 1: 723720-726407, 739080-740999 Subtraction by Bento Soares and M. Fatima Bonaldo.

text: pooled

characteristic: organ

text: Homo sapiens

text: Not I

text: Eco RI

text: phagemid (ampicillin resistant)


text: University of New Mexico

text: live

text: Generic

text: 2% sucrose for 24 hours. cDNA directionally cloned into pBluescript SK(-) using the Uni-ZAP XR vector system (Stratagene, La Jolla, CA).

text: Mycelium

text: E. coli

text: Neurospora crassa

text: EcoR I

text: Xho I

text: Mating type A

characteristic: sex

text: 74-OR23-IV A (FGSC 2489)

text: Mycelium

text: Mycelial

text: Plasmid

text: pBlueScript SK (-)


text: Children's Memorial Research Center

text: live

text: Generic

text: The UI-R-A1 library is a subtracted library derived from the UI-R-A0 library. The UI-R-A0 library consisted of a mixture of individually tagged normalized libraries constructed from rat placenta, adult lung, brain, liver, kidney, heart, spleen, ovary, and muscle. The tag is a string of 3-5 nucleotides present between the Not I site and the oligo-dT track which allows identification of the library of origin of a clone within the mixture. The subtracted library (UI-R-A1) was constructed as follows: PCR amplified cDNA inserts from a pool of approximately 3,840 UI-R-A0 clones from which 3' ESTs had been derived was used as a driver in a hybridization with the UI-R-A0 library in the form of single-stranded circles. The remaining single-stranded circles (subtracted library) was purified by hydroxyapatite column chromatography, converted to double-stranded circles and electroporated into DH10B bacteria (Life Technologies) to generate the UI-R-A1 library. This procedure has been previously described (Bonaldo, Lennon and Soares, Genome Research 6: 791-806, 1996)

characteristic: description

text: adult

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid


text: live

text: Generic

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5'-AACTGGAAGAATTCGCGGCCGCAATCGTTTTTTTTTTTTTTTTTTT-3'], double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library went through one round of normalization. Library constructed by Bento Soares and M. Fatima Bonaldo.

text: soft tissue

text: Homo sapiens

text: Not I

text: Eco RI

text: leiomyosarcoma

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCCAAGGTTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. RNA provided by Dr. Wolfgang Liedtke. Library went through two rounds of normalization, and was constructed by Bento Soares and M.Fatima Bonaldo.

text: brain

text: Mus musculus

text: Not I

text: Eco RI

text: hypothalamus

text: Soares mouse hypothalamus NMHy

text: phagemid (ampicillin resistant)


text: Livestock and Poultry Sciences Institute, USDA Agricultural Research Service

text: live

text: Generic

text: Double-stranded cDNA was ligated to EcoRI adaptors, digested with XhoI and cloned into the XhoI and EcoRI sites of the Stratagene ZAP vector. Library was not normalized.

text: 78 days into first lactation

characteristic: dev-stage

text: XLOLR

text: Bos taurus

text: XhoI

text: EcoRI

text: Female

text: Holstein

text: mammary gland

text: Bovine lactating mammary gland cDNA library

text: Phagemid

text: Stratagene ZAP Express


text: University of Texas Southwestern Medical Center at Dallas

text: live

text: Generic

text: Homo sapiens

text: Chromosome 5q33 transcripts


text: Shanghai Brilliance Biotechnology

text: live

text: Generic

text: activated dendritic

characteristic: cell type

text: Homo sapiens

text: Human activated dendritic cell mRNA


text: Purdue University

text: live

text: Generic

text: from Magnaporthe grisea nitrogen-starved mycelial culture

text: Pyricularia grisea

text: rice blast fungus mRNA from nitrogen-starved mycelial culture


text: Univ. Salamanca

text: live

text: Generic

text: Castellana

characteristic: cultivar

text: 5 days old

text: Cicer arietinum

text: etiolated epicotyls

text: chickpea mRNA CAN-5


text: Juntendo University School of Medicine

characteristic: INSDC center name

text: live

text: Generic

text: endothelial cell

text: cornea

text: Oryctolagus cuniculus

text: rabbit corneal endothelial cell


text: NHGRI, NIH

text: live

text: Generic

text: Homo sapiens

text: Human cDNA (Chandrasekharappa,S.C.)

characteristic: title


text: UTC

characteristic: INSDC first public

text: live

text: Generic

text: Zea mays

text: Maize mRNA, Stratagene catalog number 937005


text: National Cancer Institute, National Institutes of Health

characteristic: INSDC last update

text: live

text: Generic

text: Chinese hamster ovary cells

characteristic: cell-line

text: Cricetulus griseus

text: Cricetulus griseus DNA damage inducible mRNA


text: Institution of Genetics

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Homo sapiens

text: kidney

text: Human kidney (Bi,A.)


text: Institution of Genetics

text: live

text: Generic

characteristic: NCBI submission package

text: Homo sapiens

characteristic: organism

text: testis

text: Human testis (Bi,A.)


text: Institution of Genetics

text: live

text: Generic

text: Homo sapiens

text: bone marrow

characteristic: tissue-type

text: Human bone marrow (Bi,A.)


characteristic: External Id

text: Institution of Genetics

text: live

text: Generic

text: Homo sapiens

text: skeletal muscle

text: Human skeletal muscle (Bi,A.)


text: Johns Hopkins University School of Medicine

text: live

text: Generic

text: fetal

text: Homo sapiens

text: brain

text: Human fetal brain (R.L.Margolis)


text: live

text: Generic

text: Homo sapiens

text: cerebral cortex

text: Human cerebral cortex (R.L.Margolis)


text: Trinity University

text: live

text: Generic

text: produced by differential display of RNA extracted from whole ovaries of rats treated with 10 IU PMSG sc followed 48 hours later by 10 IU hCG sc to induce ovulation

text: Rattus norvegicus

text: female

text: Wistar

characteristic: strain

text: ovary

text: Rat ovary treated with PMSG/hCG


text: Tufts University Medical School

text: live

text: Generic

text: fibroblasts

text: Gallus gallus

text: tendon

text: Chicken 14v19tendon


text: Tufts University Medical School

text: live

text: Generic

text: fibroblasts

text: embryo

text: Gallus gallus

text: tendon

text: Chicken 19v14tendon


text: Tufts University Medical School

text: live

text: Generic

text: fibroblasts

text: Gallus gallus

text: tendon

text: Chicken fibroblasts


text: Centers for Disease Control and Prevention

text: live

text: Generic

text: Anopheles gambiae

text: Malaria mosquito mRNA


text: University of Heidelberg

text: live

text: Generic

text: aortic endothelial

text: cDNA library enriched for TNF-alpha suppressed genes

text: Bos taurus

text: Bos taurus aortic endothelial cell mRNA


text: The Tokyo Metropolitan Institute of Medical Science (RINSHOKEN)

text: live

text: Generic

text: adult

text: brain

characteristic: organ

text: Rattus norvegicus

text: brain

text: Rat adult brain mRNA (M.Umeda)


text: live

text: Generic

text: Rattus norvegicus

text: Rat mRNA (M.Umeda)


text: Harbin Medical University

text: live

text: Generic

text: Homo sapiens

text: lung adenocarcinoma

text: Human cell line AGZY-83a


text: John Radcliffe Hospital

text: live

text: Generic

text: Homo sapiens

text: Human chromosome 5q31-q33 mRNA


text: University of Milan

text: live

text: Generic

text: lymphocyte

text: Homo sapiens

text: Human lymphocytes cDNA (R.Meneveri)


text: University of Milan

text: live

text: Generic

text: Homo sapiens

text: small intestine

text: Human small intestine cDNA (R.Meneveri)


text: KRIBB, Genome Program

text: live

text: Generic

text: embryo

text: Mus musculus

text: Swiss Webster/NIH

text: Mouse embryo (M.Cho)


text: IMBB, Foundation for Reseach and Technology

text: live

text: Generic

text: isolated by differential display of cDNA from male mRNA; amplified by oligo-T primers from both ends; primer sequences not included

characteristic: description

text: adult

text: Anopheles gambiae

text: male

characteristic: sex

text: African malaria male mosquito GDimopoulos


text: University of Pittsburgh

text: live

text: Generic

text: Mus musculus

text: BALB/c

text: spleen

text: Mouse spleen (A.Gambotto)


text: Obihiro University of Agriculture and Veterinary Medicine

text: live

text: Generic

text: Toxoplasma gondii

text: Toxoplasma gondii mRNA (T.Koyama)


text: CINVESTAV - IPN

text: live

text: Generic

text: Leishmania mexicana mexicana

text: MHOM/MX/85/ISET HF

text: Stage-specific mRNA


text: Institute of Basic Medical Sciences

text: live

text: Generic

text: Homo sapiens

text: ovary

text: Human ovary (H. Wang)


text: Beijing Institute of Biotechnology

text: live

text: Generic

text: Jurkat

text: Homo sapiens

text: Human leukemia matchmaker, Clontech catalog #HL4015AB


text: Beijing Institute of Biotechnology

text: live

text: Generic

text: Homo sapiens

text: Human lung cancer cell line A549.A549


text: Ludwig Institute of Cancer Research

text: live

text: Generic

text: fibroblast

characteristic: cell type

text: sodium salicylate-treated; obtained by DDRT-PCR

text: embryo

characteristic: dev-stage

text: Mus musculus

text: Mouse sodium salicylate-treated fibroblast mRNA


text: Wageningen Agricultural University

characteristic: INSDC center name

text: live

text: Generic

text: embryo

text: Gallus gallus

text: Chick embryo, Stratagene number 937405

text: Uni-ZAP TMXR Vector

characteristic: vector


text: University of Bristol

characteristic: INSDC first public

text: live

text: Generic

text: Felis catus

text: Felis catus partial mRNA

characteristic: title


text: CSIRO

characteristic: INSDC last update

text: live

text: Generic

text: Bos taurus

text: muscle

text: Bovine mRNA, Stratagene cat no. 937721


text: Northeastern Ontario Regional Cancer Centre

text: live

characteristic: INSDC status

text: Generic

text: HSG

characteristic: cell-line

text: Homo sapiens

text: salivary gland

text: Human salivary gland cell line HSG


text: Institute of Basic Medical Sciences, Peking Union Medical College

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Rattus norvegicus

text: Wistar

text: testis seminiferous tubule

text: Rat testis seminiferous tubule


text: University of Parma

text: live

text: Generic

text: Tuber borchii

characteristic: organism

text: mycelia

characteristic: tissue-type

text: Tuber borchii mycelia


characteristic: External Id

text: Henry Ford Hospital

text: live

text: Generic

text: 69-77 days adult

text: Rattus norvegicus

text: male

text: Wistar

characteristic: strain

text: brain

text: Rat brain, targeted mRNA display


text: Martin Luther University

text: live

text: Generic

text: T cell

text: Jurkat

text: cell line stimulated by contact with synoviocytes

characteristic: description

text: Homo sapiens

text: Human Jurkat cell line mRNA (Thiele,K.)


text: University of Pittsburgh

text: live

text: Generic

text: Rattus norvegicus

text: Lewis

text: muscle

text: Lewis rat muscle mRNA


text: Case Western Reserve University

text: live

text: Generic

text: Russ Finley's RFLY1 produced in Roger Brent's laboratory at Massachusetts General Hospital

text: 0-12 hour embryo

text: Drosophila melanogaster

text: RFLY1 (R.Finley)


text: San Francisco General Hospital-University of California, San Francisco

text: live

text: Generic

text: a lambda ZAP-cDNA library obtained through the NIH AIDS Research and Reference Reagent Program

text: tachyzoite

text: Toxoplasma gondii

text: RH

text: lambda ZAP


text: Cellular Genetics Unit, Universite Catholique de Louvain, Ludwig Institute for Cancer Research, Brussels Branch

text: live

text: Generic

text: Homo sapiens

text: male

characteristic: sex

text: testis

text: Human testis (C. De Smet)


text: UMR CNRS 6553

text: live

text: Generic

text: Brassica napus

text: oleifera

text: Brassica napus mRNA (Deleu,C.)


text: University of Illinois at Urbana-Champaign

text: live

text: Generic

text: Homo sapiens

text: Human mRNA (H.M.Robertson)


text: Roswell Park Cancer Institute

text: live

text: Generic

text: Mus musculus

text: testis

text: Mouse testis-specific mRNA (M.F.Kulesz-Martin)


text: Smith College Department of Biological Sciences

text: live

text: Generic

text: Lymphatic filarial nematode parasite of humans. cDNA was amplified by PCR using T3 and T7 primers from conventionally constructed libraries: L3 (SAW94WL-BmL3), microfilaria (SAW94LS-BmMf), adult male (SAW94NL-BmAm) and adult female (SAW96MLW-BmAf). Microfilaria, adult male and adult female cDNAs were subtracted from L3 cDNA using the PCR-select cDNA subtraction kit (Clontech). The library has 2.6 x 106 independent recombinants and the average insert size is ~500bp. The library was constructed by Lori Saunders. The library is available from Dr. S. A. Williams, email: genome@smith.edu

text: L3 cDNA subtracted with microfilaria, adult male and adult female cDNA libraries

text: XL1-Blue MRF'

characteristic: lab-host

text: Brugia malayi

text: Eco RI

text: Brugia malayi L3 subtracted cDNA library (SAW97LS-BmL3SA)

text: Phage

text: Lambda ZAP Express


text: University of Georgia, College of Veterinary Medicine

text: live

text: Generic

text: Bos taurus

text: skeletal muscle

text: Bovine skeletal muscle cDNAs


text: live

text: Generic

text: fetal

characteristic: dev-stage

text: Bos taurus

text: thigh muscle

text: Bovine fetal thigh muscle cDNAs


text: Zhejiang Agri. Univ.

characteristic: INSDC center name

text: live

text: Generic

text: Oryza sativa

text: Rice mRNA (Li,Debao)


text: University of Naples

text: live

text: Generic

text: PEER

text: Homo sapiens

text: Human mRNA (G. La Mantia)


text: live

text: Generic

text: Lymphatic filarial nematode parasite of humans. cDNA inserts were obtained by subtracting the JHU93SL-BmL3 cDNA library (constructed using SL sequence and PCR) from SAW94WL-BmL3 cDNA library (constructed using conventional cDNA methods). cDNA was amplified from both libraries using PCR and SL cDNA clones were eliminated through hybridization. The library has 5 x 10E6 independent recombinants and the average insert size is ~ 600 bp. The library was constructed by Ying Gong. The library is available from Dr.S.A.Williams, email: genome@smith.edu.

text: conventional L3 cDNA minus spliced-leader L3 cDNA

text: XL1-Blue MRF'

text: Brugia malayi

text: Eco RI

text: Eco RI

text: Brugia malayi L3 subtracted cDNA library (SAW97YG-BmL3SB)

text: Phage

text: Lambda ZAP II (Stratagene)

characteristic: vector


text: Pohang University of Science and Technology

characteristic: INSDC first public

text: live

text: Generic

text: Fuji

characteristic: cultivar

text: initial pfu: 2*106;average insert size:1.3 Kb

text: Malus domestica

text: peel

text: Apple peel cDNA library

text: phage

text: Lambda vector UniZap II


characteristic: INSDC last update

text: live

text: Generic

text: Fuji

text: initial pfu: 6.95*106;average insert size:1.7 Kb

text: Malus domestica

text: carpel

text: Apple carpel cDNA library

characteristic: title

text: phage

text: Lambda vector UniZap II


text: live

characteristic: INSDC status

text: Generic

text: Fuji

text: initial pfu:7*106;average insert size: 0.85

text: 7-10 days after anthesis

text: Malus domestica

text: Apple young fruit cDNA library

text: phage

text: Lambda vector UniZap II


text: Mott Center for Human Growth and Development, Wayne State University School of Medicine

text: live

text: Generic

characteristic: NCBI submission model

text: preimplantation embryo; identified by differential display using Random Arbitrary Primer (RAP) method

text: morula

text: Mus musculus

text: Mouse morula differential display product (U.K.Rout)


text: University of Leeds

text: live

text: Generic

characteristic: NCBI submission package

text: enamel organ

characteristic: organ

text: Rattus norvegicus

text: male

text: Wistar

text: Rat enamel organ mRNA


text: National Heart Lung and Blood Institute

text: live

text: Generic

text: adult

text: Bos taurus

characteristic: organism

text: medulla oblongata

characteristic: tissue-type

text: Bovine medulla oblongata expressed sequence


characteristic: External Id

text: Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry

text: live

text: Generic

text: T-cell

characteristic: cell type

text: adult

text: Homo sapiens

text: Human T-cell cDNA library (M.G.Smirnova)


text: Christchurch School of Medicine

text: live

text: Generic

text: embryonic stem cell

characteristic: cell-line

text: embryo

text: Mus musculus

text: male

characteristic: sex

text: Mouse embryonic stem cell (M.A.Kennedy)


text: Mount Sinai Medical Center

text: live

text: Generic

text: Mus musculus

text: Swiss

characteristic: strain

text: Mouse v-src-suppressed mRNA (I.H.Gelman)


text: National Institute of Neurological Disorders and Stroke

characteristic: INSDC center name

text: live

text: Generic

characteristic: dev-stage

text: Rattus norvegicus

text: spinal cord

text: Rat spinal cord mRNA (R.L.Somogyi)


text: University of Alabama at Birmingham

characteristic: INSDC first public

text: live

text: Generic

text: 2 year old

text: Homo sapiens

text: female

text: hippocampus

text: Human hippocampus, Stratagene catalog #936205


text: Immunex Corporation

text: live

text: Generic

text: Homo sapiens

text: Human cDNA from Wiskott-Aldrich syndrome gene region


text: IRO

text: live

text: Generic

text: Homo sapiens

text: Human chromosome 21q22 mRNA


text: Universidade Federal de Minas Gerais

text: live

text: Generic

text: primary cell culture

text: cDNA was obtained by differential display RT-PCR from a primary cell culture of a skin lesion from a patient with systemic sclerosis

characteristic: description

text: Homo sapiens

text: female

text: skin

text: Human skin lesion from patient with systemic sclerosis


text: Experimental Therapeutics

characteristic: INSDC last update

text: live

text: Generic

text: fibroblast

text: Homo sapiens

text: foreskin

text: Human TNF-treated BG9 fibroblasts


text: Purdue University

text: live

text: Generic

text: 10 day old

text: Solanum lycopersicum

text: Rutgers

text: fruit pericarp

text: Tomato fruit pericarp, 10 day old

characteristic: title


text: Purdue University

text: live

characteristic: INSDC status

text: Generic

text: 20 day old

text: Solanum lycopersicum

text: Rutgers

text: fruit pericarp

text: Tomato fruit pericarp, 20 day old


text: Jewish Hospital at Washington University Medical Center

text: live

text: Generic

characteristic: NCBI submission model

text: adult

text: Brugia malayi

text: Brugia malayi cDNA (R.Chandrashekar)


text: live

text: Generic

characteristic: NCBI submission package

text: adult

text: Onchocerca volvulus

text: Onchocerca volvulus cDNA (R.Chandrashekar)


text: Universitaet Ulm

text: live

text: Generic

text: primary melanocytes

text: a library consisting of unspecifically PCR-amplified 3' ends of mRNA from primary human melanocytes cultured in vitro. The PCR-products were cloned in puc18 and transformed to E.coli JM 103. The sequence of this clone was obtained using M13 universal primers by one single read

text: Homo sapiens

text: female

text: Human primary melanocytes mRNA (I.M.Eisenbarth)

characteristic: vector


text: live

text: Generic

text: leukocyte

text: Homo sapiens

characteristic: organism

text: Human leukocyte (M.L.Markelov)


text: University of Tsukuba

text: live

text: Generic

text: 36 years old

text: Homo sapiens

text: female

text: brain

characteristic: tissue-type

text: Human female brain (Life Technologies)


characteristic: External Id

text: Medizinische Klinik 1

text: live

text: Generic

text: fetal

text: Homo sapiens

text: brain

text: Human fetal brain cDNA (T.M.Gress)


text: Beijing Medical University

text: live

text: Generic

text: Mus musculus

text: bone marrow

text: Mouse C57 BL-6 bone marrow cDNA (W.L.Han)


text: Beijing Medical University

text: live

text: Generic

text: Mus musculus

text: BXSB

text: bone marrow

text: Mouse BXSB bone marrow cDNA (W.L.Han)


text: Kinki University

text: live

text: Generic

text: slug

text: Dictyostelium discoideum

text: Dictyostelium discoideum SS (M.Yoshida)


text: The Institute for Genomic Research

text: live

text: Generic

text: brain

characteristic: organ

text: Rattus sp.

text: EcoRI

text: NotI

text: Normalized rat brain, Bento Soares


text: The Institute for Genomic Research

text: live

text: Generic

text: embryo 8, 12, 18 dpc

text: Rattus sp.

text: EcoRI

text: NotI

text: Normalized rat embryo, Bento Soares


text: The University of Tokyo

text: live

text: Generic

text: Oryza sativa

text: callus

text: Rice callus cDNA (H.Uchimiya)


text: Lawrence Livermore National Laboratory

text: live

text: Generic

text: fetal

text: Homo sapiens

text: brain

text: ICRFHFB


text: University College London

text: live

text: Generic

text: fetal

text: Homo sapiens

text: brain

text: Human fetal brain (S.E.Mole)


text: Horticulture Research International

text: live

text: Generic

text: Golden Harvest

characteristic: cultivar

text: Narcissus pseudonarcissus

text: flower bud

text: Daffodil Flower-1


text: Baylor College of Medicine

text: live

text: Generic

text: Homo sapiens

text: male

characteristic: sex

text: colorectal cancer

text: Human colorectal cancer (Genome Res. 1995)


text: live

text: Generic

text: Burkitt's lymphoma

characteristic: cell type

characteristic: cell-line

text: Sanders,R., Larsson,C., Wallin,E., Islam,K.B. and Smith,I.E. Partial sequencing and chromosome mapping of clones from a human Burkitt's lymphoma cDNA library (Unpublished 1994)

text: Homo sapiens

text: BL29 Burkitt's lymphoma (K.M.Timms)


text: Lawrence Livermore National Laboratory

characteristic: INSDC center name

text: live

text: Generic

text: NSTH II

text: adult

characteristic: dev-stage

text: Homo sapiens

text: thymus

text: Human adult thymus NSTH II


text: Children's Memorial Research Center

characteristic: INSDC first public

text: live

text: Generic

text: The UI-R-E1 library is a subtracted library derived from the UI-R-E0 library. The UI-R-E0 library consisted of a mixture of individually tagged normalized libraries constructed from 8, 12 and 18-day embryo. The tag is a string of 3-5 nucleotides present between the Not I site and the oligo-dT track which allows identification of the library of origin of a clone within the mixture. The subtracted library (UI-R-E1) was constructed as follows: PCR amplified cDNA inserts from a pool of UI-R-E0 clones from which 3' ESTs had been derived was used as a driver in a hybridization with the UI-R-E0 library in the form of single-stranded circles. The remaining single-stranded circles (subtracted library) was purified by hydroxyapatite column chromatography, converted to double-stranded circles and electroporated into DH10B bacteria (Life Technologies) to generate the UI-R-E1 library. This procedure has been previously described (Bonaldo, Lennon and Soares, Genome Research 6: 791-806, 1996)

characteristic: description

text: adult

text: DH10B (Life Technologies)

characteristic: lab-host

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

characteristic: strain

text: Phagemid


text: University of Manitoba

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Glacier

text: mRNA from B. napus cv. Glacier leaves infiltrated with Leptosphaeria maculans PG2 pycnidiospores for 48 hr. (incompatible interaction).

text: leaf

text: Brassica napus

text: EcoRI

text: XhoI

characteristic: title

text: plasmid

text: Uni-ZAPXR (Stratagene)


text: Faculty of Medicine Tottori University

text: live

text: Generic

characteristic: NCBI submission package

text: adult

text: Rattus norvegicus

characteristic: organism

text: Wistar

text: seminal vesicle

characteristic: tissue-type

text: Rat seminal vesicle (M.Izawa)


characteristic: External Id

text: Institute de Biotechnologie des Plantes

text: live

text: Generic

text: 2 week old

text: Arabidopsis thaliana

text: ecotype Columbia GH50

text: shoots

text: Arabidopsis thaliana 2 week old shoots

text: lambda ZAPII

characteristic: vector


text: University of Sydney

text: live

text: Generic

text: 3 months axenic culture with arbuscular mycorrhizal fungus Gigaspora margarita

text: Daucus carota

text: root transformed with root-inducing Agrobacterium rhizogenes

text: Daucus carota root (B.R.Lyon)


text: University of Sydney

text: live

text: Generic

text: From root transformed with root-inducing Agrobacterium rhizogenes; organism which is the source of the sequence has not been determined by Southern analysis

text: Daucus carota/Gigaspora margarita mixed EST library


text: Univ. Salamanca

text: live

text: Generic

text: Castellana

characteristic: cultivar

text: 3 days old

text: Cicer arietinum

text: etiolated and osmotic stressed epicotyl

text: chickpea mRNA CAP-4


text: Livestock and Poultry Sciences Institute, USDA Agricultural Research Service

text: live

text: Generic

text: Double-stranded cDNA was ligated to EcoRI adaptors, digested with XhoI and cloned into the EcoRI and XhoI sites of the Stratagene Lambda ZAP vector. Library was not normalized.

text: 90 days into gestation

text: Bos taurus

text: EcoRI

text: XhoI

text: Female

characteristic: sex

text: Hereford

text: Placentome

text: Bovine placentome (day 90)

text: phagemid

text: Stratagene Lambda ZAP


text: Universitaet Bielefeld

characteristic: INSDC center name

text: live

text: Generic

text: Gerbel

text: 8 day old seedlings

text: Hordeum vulgare subsp. vulgare

text: basal 5 mm of the primary leaf

text: Barley leaf, 8 day old seedling

text: lambda ZAP


text: Children's Memorial Research Center

text: live

text: Generic

text: The UI-R-C0 library is a subtracted library derived from the UI-R-A1 and UI-R-E1 libraries. The UI-R-A1 library consisted of a mixture of individually tagged normalized libraries constructed from rat placenta, adult lung, brain, liver, kidney, heart, spleen, ovary, and muscle. The UI-R-E1 library consisted of a mixture of individually tagged normalized libraries constructed from 8, 12 and 18-day embryo. The tag is a string of 3-5 nucleotides present between the Not I site and the oligo-dT track which allows identification of the library of origin of a clone within the mixture. The subtracted library (UI-R-C0) was constructed as follows: PCR amplified cDNA inserts from a pool of UI-R-A1 and UI-R-E1 clones from which 3' ESTs had been derived was used as a driver in a hybridization with the pooled UI-R-A1 and UI-R-E1 library in the form of single-stranded circles. The remaining single-stranded circles (subtracted library) was purified by hydroxyapatite column chromatography, converted to double-stranded circles and electroporated into DH10B bacteria (Life Technologies) to generate the UI-R-C0 library. This procedure has been previously described (Bonaldo, Lennon and Soares, Genome Research 6: 791-806, 1996)

text: adult

characteristic: dev-stage

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid


text: Virginia Tech

characteristic: INSDC first public

text: live

text: Generic

text: Meleagris gallopavo

text: pituitary

text: Turkey pituitary cDNA library


text: Labs Fournier

text: live

text: Generic

text: AP20 melanoma

characteristic: cell-line

text: Homo sapiens

text: AP20 melanoma mRNA


text: Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, and Peking Union Medical College

text: live

text: Generic

text: lymphocyte

characteristic: cell type

text: Homo sapiens

text: Human lymphocyte mRNA (Zhu,L.-P.)


text: University of Ilinois

text: live

text: Generic

text: Zea mays

characteristic: strain

text: Maize strain L477 mRNA


text: Washington University School of MedicineP

characteristic: INSDC last update

text: live

text: Generic

text: 1st strand cDNA was primed with an oligo(dT) primer [ATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded cDNA was ligated to a DraIII adaptor [TGTTGGCCTACTGG], digested and cloned into distinct DraIII sites of the pME18S-FL3 vector (5' site CACTGTGTG, 3' site CACCATGTG). XhoI should be used to isolate the cDNA insert. Size selection was performed to exclude fragments <1.5kb. Library constructed by Dr. Sumio Sugano (University of Tokyo Institute of Medical Science). Custom primers for sequencing: 5' end primer CTTCTGCTCTAAAAGCTGCG and 3' end primer CGACCTGCAGCTCGAGCACA.

characteristic: description

text: embryo, 14 dpc

characteristic: lab-host

text: Mus musculus

text: DraIII (CACTGTGTG)

text: DraIII (CACCATGTG)

text: Sugano mouse embryo mewa

text: phagemid (ampicillin resistant)


text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: adult

text: liver

characteristic: organ

text: Mus musculus

text: DraIII (CACTGTGTG)

text: DraIII (CACCATGTG)

text: female

text: Sugano mouse liver mlia

characteristic: title

text: phagemid (ampicillin resistant)


characteristic: External Id

text: Washington University School of Medicine

text: live

text: Generic

characteristic: NCBI submission package

text: adult

text: kidney

text: Mus musculus

characteristic: organism

text: DraIII (CACTGTGTG)

text: DraIII (CACCATGTG)

text: female

text: Sugano mouse kidney mkia

text: phagemid (ampicillin resistant)


text: Shanghai Tiedao University Medical School

text: live

text: Generic

text: mRNA was purified from two operative specimens of human glioma. The human glioma library was constructed by in vitro packing. The library contained 1.02E06 recombinants. Percentage of recombinants was 96.2%. The work was done by Prof. Yao Wang

text: Homo sapiens

text: male

characteristic: sex

text: brain

characteristic: tissue-type

text: human glioblastoma library


text: Beijing Institute of Radiation

characteristic: INSDC center name

text: live

text: Generic

text: mononucleated cell

text: Rattus norvegicus

text: Wistar

text: bone marrow

text: Rat bone marrow mononucleated cell


text: Cancer Research Institute, Hunan Medical University

characteristic: INSDC first public

text: live

text: Generic

text: Homo sapiens

text: neuroepithelium

text: Human neuroepithelium (N.Jiang)


text: Mayo Clinic Scottsdale

text: live

text: Generic

text: RAP-RT-PCR product from posterior region

text: 9.5 dpc embryo

characteristic: dev-stage

text: Mus musculus

text: FVB

text: Mouse 9.5 dpc embryo


text: Iowa State University

text: live

text: Generic

characteristic: cultivar

text: ds-cDNA molecules were generated as follows. First-strand cDNA was prepared from oligo-dT selected mRNA by priming with an XhoI oligo-dT primer. The resulting DNA:RNA hybrid was treated with RNase H and used as a template for DNA PolI-catalyzed second strand synthesis. After the addition of EcoRI adaptors, the ds-cDNAs were digested with XhoI and size-selected. The resulting molecules were directionally cloned into the EcoRI and XhoI sites of the pET30a plasmid (Novagen).

text: two-week-old green seedling

text: green seedlings

text: Zea mays

text: EcoRI

text: XhoI

text: above ground tissues

text: plasmid

characteristic: vector