characteristic: External Id

text: live

text: Generic

text: cDNA library was constructed from root tissues of 25 days after inoculated (DAI) pigeonpea seedlings of ICPL20102 genotype imposed to Fusarium wilt stress in greenhouse conditions. The cDNA fragments were cloned into pGEM-T Easy vector and subsequently sequenced by Sanger sequencing approach using M13 primers.

text: E. coli (DH5a)

text: Cajanus cajan

text: EcoRI

text: root

text: Fusarium wilt challenged (25 DAI) pigeonpea genotype ICPL20102 root cDNA library

text: plasmid

text: pGEM-T Easy vector


status: PUBLIC

text: International Crops Research Institute for the Semi-Arid Tropics (ICRISAT)

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

tag: attribute

characteristic: cultivar

text: cDNA library was constructed from root tissues of 30 days after inoculated (DAI) pigeonpea seedlings of ICPL20102 genotype imposed to Fusarium wilt stress in greenhouse conditions. The cDNA fragments were cloned into pGEM-T Easy vector and subsequently sequenced by Sanger sequencing approach using M13 primers.

characteristic: description

text: E. coli (DH5a)

characteristic: lab-host

text: Cajanus cajan

characteristic: organism

text: EcoRI

text: root

characteristic: tissue-type

text: Fusarium wilt challenged (30 DAI) pigeonpea genotype ICPL20102 root cDNA library

characteristic: title

text: plasmid

text: pGEM-T Easy vector

characteristic: vector


text: live

text: Generic

text: cDNA library was constructed from root tissues of 6 days after inoculated (DAI) pigeonpea seedlings of ICP2376 genotype imposed to Fusarium wilt stress in greenhouse conditions. The cDNA fragments were cloned into pGEM-T Easy vector and subsequently sequenced by Sanger sequencing approach using M13 primers.

text: E. coli (DH5a)

text: Cajanus cajan

text: EcoRI

text: root

text: Fusarium wilt challenged (6 DAI) pigeonpea genotype ICP2376 root cDNA library

text: plasmid

text: pGEM-T Easy vector


text: live

text: Generic

text: cDNA library was constructed from root tissues of 10 days after inoculated (DAI) pigeonpea seedlings of ICP2376 genotype imposed to Fusarium wilt stress in greenhouse conditions. The cDNA fragments were cloned into pGEM-T Easy vector and subsequently sequenced by Sanger sequencing approach using M13 primers.

text: E. coli (DH5a)

text: Cajanus cajan

text: EcoRI

text: root

text: Fusarium wilt challenged (10 DAI) pigeonpea genotype ICP2376 root cDNA library

text: plasmid

text: pGEM-T Easy vector


text: live

text: Generic

text: cDNA library was constructed from root tissues of 15 days after inoculated (DAI) pigeonpea seedlings of ICP2376 genotype imposed to Fusarium wilt stress in greenhouse conditions. The cDNA fragments were cloned into pGEM-T Easy vector and subsequently sequenced by Sanger sequencing approach using M13 primers.

text: E. coli (DH5a)

text: Cajanus cajan

text: EcoRI

text: root

text: Fusarium wilt challenged (15 DAI) pigeonpea genotype ICP2376 root cDNA library

text: plasmid

text: pGEM-T Easy vector


text: live

text: Generic

text: cDNA library was constructed from root tissues of 20 days after inoculated (DAI) pigeonpea seedlings of ICP2376 genotype imposed to Fusarium wilt stress in greenhouse conditions. The cDNA fragments were cloned into pGEM-T Easy vector and subsequently sequenced by Sanger sequencing approach using M13 primers.

text: E. coli (DH5a)

text: Cajanus cajan

text: EcoRI

text: root

text: Fusarium wilt challenged (20 DAI) pigeonpea genotype ICP2376 root cDNA library

text: plasmid

text: pGEM-T Easy vector


text: live

text: Generic

text: cDNA library was constructed from root tissues of 25 days after inoculated (DAI) pigeonpea seedlings of ICP2376 genotype imposed to Fusarium wilt stress in greenhouse conditions. The cDNA fragments were cloned into pGEM-T Easy vector and subsequently sequenced by Sanger sequencing approach using M13 primers.

text: E. coli (DH5a)

text: Cajanus cajan

text: EcoRI

text: root

text: Fusarium wilt challenged (25 DAI) pigeonpea genotype ICP2376 root cDNA library

text: plasmid

text: pGEM-T Easy vector


text: live

text: Generic

text: cDNA library was constructed from root tissues of 30 days after inoculated (DAI) pigeonpea seedlings of ICP2376 genotype imposed to Fusarium wilt stress in greenhouse conditions. The cDNA fragments were cloned into pGEM-T Easy vector and subsequently sequenced by Sanger sequencing approach using M13 primers.

text: E. coli (DH5a)

text: Cajanus cajan

text: EcoRI

text: root

text: Fusarium wilt challenged (30 DAI) pigeonpea genotype ICP2376 root cDNA library

text: plasmid

text: pGEM-T Easy vector


text: live

text: Generic

text: cDNA library was constructed from leaf tissues of 45 days after sowing (DAS) pigeonpea seedlings of ICP7035 genotype imposed to Sterility mosaic disease (SMD) stress in greenhouse conditions. The cDNA fragments were cloned into pGEM-T Easy vector and subsequently sequenced by Sanger sequencing approach using M13 primers.

text: E. coli (DH5a)

text: Cajanus cajan

text: EcoRI

text: leaf

text: Sterility mosaic disease (SMD) challenged (45 DAS) pigeonpea genotype ICP7035 leaf cDNA library

text: plasmid

text: pGEM-T Easy vector


text: International Crops Research Institute for the Semi-Arid Tropics (ICRISAT)

text: live

text: Generic

text: cDNA library was constructed from leaf tissues of 60 days after sowing (DAS) pigeonpea seedlings of ICP7035 genotype imposed to Sterility mosaic disease (SMD) stress in greenhouse conditions. The cDNA fragments were cloned into pGEM-T Easy vector and subsequently sequenced by Sanger sequencing approach using M13 primers.

text: E. coli (DH5a)

text: Cajanus cajan

text: EcoRI

text: leaf

text: Sterility mosaic disease (SMD) challenged (60 DAS) pigeonpea genotype ICP7035 leaf cDNA library

text: plasmid

text: pGEM-T Easy vector


text: live

text: Generic

text: cDNA library was constructed from leaf tissues of 45 days after sowing (DAS) pigeonpea seedlings of TTB7 genotype imposed to Sterility mosaic disease (SMD) stress in greenhouse conditions. The cDNA fragments were cloned into pGEM-T Easy vector and subsequently sequenced by Sanger sequencing approach using M13 primers.

text: E. coli (DH5a)

text: Cajanus cajan

text: EcoRI

text: leaf

text: Sterility mosaic disease (SMD) challenged (45 DAS) pigeonpea genotype TTB7 leaf cDNA library

text: plasmid

text: pGEM-T Easy vector


text: live

text: Generic

text: cDNA library was constructed from leaf tissues of 60 days after sowing (DAS) pigeonpea seedlings of TTB7 genotype imposed to Sterility mosaic disease (SMD) stress in greenhouse conditions. The cDNA fragments were cloned into pGEM-T Easy vector and subsequently sequenced by Sanger sequencing approach using M13 primers.

text: E. coli (DH5a)

text: Cajanus cajan

text: EcoRI

text: leaf

text: Sterility mosaic disease (SMD) challenged (60 DAS) pigeonpea genotype TTB7 leaf cDNA library

text: plasmid

text: pGEM-T Easy vector


text: MPI for Molecular Genetics

text: live

text: Generic

text: collected_by: Christoph Bleidorn; collection_date: 21-May-2007; country: France:Britanny, Morgat; lat_lon: 48.23 N 4.5 W; Total RNA was extracted from 100 individuals. Collected tissue frozen in liq N2 and stored at -80C prior to RNA extraction. An amplified cDNA library was constructed CloneMiner (Invitrogen). cDNA was size fractioned and directional cloned using the vector pDNR-LIB. Clones containing cDNA inserts were sequenced from the 5' end on the automated capillary sequencer systems ABI 3730 XL (Applied Biosystems, Darmstadt, Germany) and MegaBace 4500 (GE Healthcare, Muenchen, Germany) using BigDye chemistry. Library contruction and sequencing were performed at the MPI for Molecular Genetics Berlin.

text: adult

characteristic: dev-stage

text: Myzostoma cirriferum

text: protandric hermaphrodite

characteristic: sex

text: whole animal

text: Myzostoma cirriferum whole animal adult

text: pDNR-LIB


text: Department of Bioengineering & Environmental Science, Changsha University

text: live

text: Generic

text: adult

text: Siniperca chuatsi

text: muscle

text: mandarin fish muscle library


text: National Institute of Allergy and Infectious Diseases

text: live

text: Generic

text: Unidirectional library was constructed using long distance PCR (Smart cDNA kit) and Lambda TriplEx2 vector

text: adult female

text: salivary glands

characteristic: organ

text: Ochlerotatus triseriatus

text: female

text: Adult female salivary gland cDNA library Octrisg1

text: Phage

text: Lambda TriplEx2


characteristic: External Id

text: College of Life Science and Technology, Southwest University for Nationality

text: live

text: Generic

text: Screening and identification of differentially expressed genes in chicken spleen infected with Newcastle Disease Virus by suppression subtractive hybridization.

text: 15-day-old chicken

text: Gallus gallus

text: White Leghorn

characteristic: strain

text: spleen

text: subtractive Library of chicken spleen 36h post-infected NDV


text: National Academy of Agricultural Science, RDA

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: Harmonia axyridis

text: Harmonia axyridis antibacterial related cDNA library


text: School of Environmental and Natural Resource Sciences, Faculty of Science and Technology

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: ElectroMAX DH10B

text: Tree trunk and branches

text: Acacia auriculiformis x Acacia mangium

text: Inner bark

text: Acacia hybrid EST Library


text: Institute of Plant Protection, Chinese Academy of Agricultural Sciences

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Mixed

characteristic: cell type

text: three days after eclosion

text: Adelphocoris lineolatus

text: Female

text: antennae

text: Antennae cDNA libraries of the Lucerne Plant bug, Female

text: pDNR-LIB


text: live

text: Generic

text: Mixed

text: three days after eclosion

text: Adelphocoris lineolatus

text: Male

text: antennae

text: Antennae cDNA libraries of the Lucerne Plant bug, Male

text: pDNR-LIB


text: Faculdade de Ciencias Farmaceuticas, Universidade de Sao Paulo

text: live

text: Generic

text: Golden

characteristic: cultivar

text: Papaya fruit were harvested at color break to 25% yellow. Ripening was induced by exogenous ethylene: fruit were left in a 240 dm3 chamber and exposed to a concentration of 100 ppm ethylene in a constant flow-through system during 2 h, and 10 h in a closed system. Another group had the ripening delayed by 1-MCP treatment: fruit were left in a 334 dm3 chamber and exposed to a concentration of 100 ppb 1-MCP, for 12 hours in a closed system (Fabi et al. 2007). After treatment, fruit were ventilated and left to ripe under controlled temperature and humidity. cDNA-AFLP was performed using a total of 64 primer pairs combinations according to the instructions described by Bachem et al (1996). The samples were compared as non-treated against ethylene treated against 1-MCP treated.

characteristic: description

text: 150 days after anthesis

text: E.coli

characteristic: lab-host

text: Fruit

text: Carica papaya

text: Hermaphrodite

text: Pulp

text: Induced and delayed ripening papaya pulp

text: Plasmid

text: pGEM-EASY


text: Instituto Oswaldo Cruz

text: live

text: Generic

text: Enrichment for A. aquasalis differentially expressed genes during parasite development by subtractive hybridization. The library represents 2 hours non-infected minus P. vivax infected A. aquasalis expressed genes.

text: Anopheles aquasalis/Plasmodium vivax mixed EST library

characteristic: organism

characteristic: title

text: pGEM T-easy cloning vector


text: Instituto Oswaldo Cruz

text: live

text: Generic

text: Enrichment for A. aquasalis differentially expressed genes during parasite development by subtractive hybridization. The library represents 2 hours P. vivax infected minus non-infected A. aquasalis expressed genes.

text: pGEM T-easy cloning vector

characteristic: vector


text: Instituto Oswaldo Cruz

text: live

text: Generic

text: Enrichment for A. aquasalis differentially expressed genes during parasite development by subtractive hybridization. The library represents 24 hours non-infected minus P. vivax infected A. aquasalis expressed genes.

text: pGEM T-easy cloning vector


text: Instituto Oswaldo Cruz

text: live

text: Generic

text: Enrichment for A. aquasalis differentially expressed genes during parasite development by subtractive hybridization. The library represents 24 hours P. vivax infected minus non-infected A. aquasalis expressed genes.

text: pGEM T-easy cloning vector


text: Instituto de Biologia Molecular y Celular de Rosario

text: live

text: Generic

text: petit havana

text: These are single read cDNA-AFLP sequences

text: 2-8-24 hs post inoculation

characteristic: dev-stage

text: Nicotiana tabacum

text: young leaves

characteristic: tissue-type

text: Nicotiana tabacum during Xac interaction


characteristic: External Id

text: Nanjing Agricultural University

text: live

text: Generic

text: suzhouqing

text: country: China

text: Brassica rapa subsp. chinensis

text: Brassica rapa subsp. chinensis cv. suzhouqing library


text: USDA-ARS

text: live

text: Generic

text: Subtractive hybridization was performed with the PCR-Select cDNA Subtraction Kit (Clontech), according to the manufacture's protocol. Tester consisted of the modified live vaccine strain RE-33. Driver was the virulent strain AL93-58.

text: Edwardsiella ictaluri

text: RE33 and AL93-58

characteristic: strain

text: Modified live Edwardsiella ictaluri vaccine strain RE33 vs virulent strain AL93-58 subtractive cDNA library

text: pGEM T easy (promega)


text: Agriculture and Agri-food Canada

characteristic: INSDC center name

text: live

text: Generic

text: aeciospores

text: Isolate 99193, telia collected from bread wheat, Triticum aestivum, cultivar Bet Hashita (June 2004; Y. Anikster and T. Eilam, Institute for Cereal Crops Improvement, George S. Wise Faculty of Life Sciences, Tel Aviv University, Israel). RNA obtained by grinding aeciospores with Trizol; cDNA preparation using SMART cDNA library kit (Clontech). After 52 PCR cycles, cDNA was digested with SfiI, size-fractionated and cloned in vector pDNR-LIB.

text: resting aeciospores resulting from inoculation of Thalictrum speciosissimum by suspended telia

characteristic: isolate

text: E. coli DH10B/r

text: Puccinia triticina

text: SfiI

text: SfiI

text: Plasmid

text: pDNR-LIB


text: Agriculture and Agri-food Canada

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: pycniospores

text: Isolate 99193, telia collected from bread wheat, Triticum aestivum, cultivar Bet Hashita (June 2004; Y. Anikster and T. Eilam, Institute for Cereal Crops Improvement, George S. Wise Faculty of Life Sciences, Tel Aviv University, Israel). RNA obtained by grinding aeciospores with Trizol; cDNA preparation using SMART cDNA library kit (Clontech). After 26 PCR cycles, cDNA was digested with SfiI, size-fractionated and cloned in vector pDNR-LIB.

text: resting pycniospores resulting from inoculation of Thalictrum speciosissimum by suspended telia

text: E. coli DH10B/r

text: Puccinia triticina

text: SfiI

text: SfiI

text: Plasmid

text: pDNR-LIB


text: Agriculture and Agri-food Canada

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: teliospores

text: RNA obtained by grinding dissected teliospore pustules with Trizol; cDNA preparation using SMART cDNA library kit (Clontech). After 26 PCR cycles, cDNA was digested with SfiI, size-fractionated and cloned in vector pDNR-LIB.

text: resting teliospores

text: E. coli DH10B/r

text: Puccinia triticina

text: SfiI

text: SfiI

text: race 1 (designation BBBD; Long et al., 1989, Plant Disease 73, 294)

text: Plasmid

text: pDNR-LIB


text: Agriculture and Agri-food Canada

text: live

text: Generic

characteristic: NCBI submission package

text: isolated D-haustoria

characteristic: cell type

text: Isolate PRTUS3 (race PBJL; Webb et al. 2006. Funct. Integr. Genomics 6, 250-260). J. Fellers, USDA-ARS, Manhattan, KS 66506-5502, USA. Haustoria were isolated from infected wheat (Triticum aestivum) over ConA complexed columns (Hahn and Mendgen 1992. Protoplasma 170, 95-103); RNA was obtained by grinding material with phenol-guanidine isothiocyanate; cDNA preparation using oligodT primer containing a Xho1 site, adding EcoRI linkers to blunted 5'-end.

text: D-haustoria isolated from infected wheat (Triticum aestivum) cv. TAM107

text: PRTUS3 (race PBJL)

text: E. coli DH10B/r

text: Puccinia triticina

text: EcoRI (5'-end)

text: XhoI (3'-end)

text: Plasmid

text: (lambda-zap) pBluescript SK-


text: Agriculture and Agri-food Canada

text: live

text: Generic

text: urediniospores

text: Library made by M. Dickinson, University of Nottingham, Loughborough, UK. Urediniospores were germinated over water; RNA was obtained by grinding material with phenol-guanidine isothiocyanate; cDNA preparation using oligodT primer containing a Xho1 site, adding EcoRI linkers to blunted 5'-end.

characteristic: description

text: germinated urediniospores

text: WBRS-97-3 (M. Dickinson, University of Nottingham, Loughborough, UK)

text: E. coli DH10B/r

text: Puccinia triticina

text: EcoRI (5'-end)

text: XhoI (3'-end)

text: PTDG

text: Plasmid

text: (lambda-zap) pBluescript SK-


text: Agriculture and Agri-food Canada

text: live

text: Generic

text: isolated D-haustoria

text: Library made by M. Dickinson, University of Nottingham, Loughborough, UK. Haustoria were isolated from infected wheat (Triticum aestivum) over ConA complexed columns (Hahn and Mendgen 1992. Protoplasma 170, 95-103); RNA was obtained by grinding material with phenol-guanidine isothiocyanate; cDNA preparation using oligodT primer containing a Xho1 site, adding EcoRI linkers to blunted 5'-end.

text: D-haustoria isolated from infected wheat (Triticum aestivum)

text: E. coli DH10B/r

characteristic: lab-host

text: Puccinia triticina

text: EcoRI (5'-end)

text: XhoI (3'-end)

text: PTDH

text: Plasmid

text: (lambda-zap) pBluescript SK-


text: Vidya Pratishthan's School of Biotechnology

text: live

text: Generic

text: The cDNA constructed using oligo (dt)18, Superscript RT (invitrogen), ligated to an adaptor with an EcoRI-compatible overhang, cloned in pUC18

text: Young

text: E.coli DH5a

text: Leaf

characteristic: organ

text: Aegle marmelos

characteristic: organism

text: EcoRI

text: Leaf

text: Aegle Leaf

text: Plasmid


text: Institute of Oil Crops Research, Chinese Academy Agricultural Science

text: live

text: Generic

characteristic: cultivar

text: mRNA was extracted from the mixture of Brassica napus roots with low phosphorus stress for 6 h , 12 h , 24 h , 48 h ,72h, 5d, 7d, 9d. We used the mRNA differential display technique to visualize cDNA fragments derived from the phosphorus-tolerant cultivar Zy 821 and the phosphorus-sensitive cultivar No. 1182 grown under phosphorous-deficient (low-P) conditions. DDRT-PCR products were separated on a 6% urea-denaturing polyacrylamide gel. cDNA fragments was directly cloned into a pMD18-T vector and transformed into E. coli DH5alpha.

text: Brassica napus

text: root

text: Brassica napus cv. 1182 root

characteristic: title

text: Plasmid

characteristic: vector


characteristic: External Id

text: live

text: Generic

text: Brassica napus

text: leaf

characteristic: tissue-type

text: Brassica napus cv. Zy821 leaf

text: Plasmid


text: School of Bioscience and Biotechnology, Faculty of Science and Technology, National University of Malaysia

characteristic: INSDC center name

text: live

text: Generic

text: Differentiated cells

text: Approximately 2 month-old Polygonum minus in vitro plantlets were first treated with 150uM of Jasmonic acid for 3 days whereas the untreated plantlets were served as negative control. Total RNA were extracted from both populations of roots samples and converted into cDNA by reverse transcription. The treated roots were served as cDNA tester while the untreated roots were served as cDNA driver in the subtractive cDNA library preparation. The suppression subtractive hybridization technique was carried out using PCR-Select cDNA subtraction kit from Clontech, USA. 2 rounds of hybridization steps were performed to enrich the differentially expressed mRNA transcripts and to minimise the PCR background. The differentially expressed transcripts were further amplified by primer PCR and nested PCR. The secondary PCR products were then cloned into pCR2.1 vector and transformed into E. coli TOP10 to construct a subtractive cDNA library. Blue-white screening were performed and white colonies were randomly selected to analysed by PCR and reverse Northern analysis. All clones with strong hybridization signals representing jasmonic acid responsive genes were sent for sequencing. 4 potential clones responsible for aromatic compounds production, 3 clones related to abiotic stress and 1 clone representing transcription factor were selected to analysed by RT-PCR.

text: E. coli strain TOP 10

text: Roots

text: Persicaria minor

text: Jasmonic acid-treated x non-treated Polygonum minus roots subtractive Library

text: Plasmid

text: pCR 2.1


text: East China Normal University

text: live

text: Generic

text: Daphnia carinata

text: Body

text: Gamogenetic water flea (Daphnia carinata) cDNA library

text: Plasmid

text: pBluescript SK+


text: University Putra Malaysia

text: live

text: Generic

text: cDNA sequences were obtained from partial mRNA from a differential display reverse transcription-PCR (DDRT-PCR) experiment studying differential expression in C. albicans yeast and hypha forms.

text: Candida albicans

text: ATCC 14053

characteristic: strain

text: C. albicans partial mRNA library


characteristic: INSDC first public

text: live

text: Generic

text: granulosa and oocyte

text: PCR fragment; cDNA library (OF-DF) made from granulosa cells and oocytes of ovulatory follicles (OF) obtained 24 hours after human chorionic gonadotropin injection that was subtracted from granulosa and oocytes of dominant follicles (DF) collected at day 5 of the estrous cycle.

text: ovulatory follicle

characteristic: dev-stage

text: ovary

text: Bos taurus

text: female

characteristic: sex

text: Holstein

text: Bos taurus OF-DF


text: Pastoral Genomics, c/o ViaLactia Biosciences (NZ) Ltd

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Bronsyn LE

text: Immature (7 weeks after flag stage)

text: Lolium perenne

text: Monoecious

text: Seed

text: Perennial ryegrass seed immature, 7 weeks library (LPF4)

text: pBluescriptIISK+


text: DOE Joint Genome Institute

text: live

text: Generic

characteristic: NCBI submission package

text: In Vivo Conditions: light induction in several growth media. The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. The library was constructed and sequencing performed at the DOE Joint Genome Institute (Walnut Creek, CA).

characteristic: description

text: Mucor circinelloides

text: SalI

text: NotI

text: whole mycelium

text: CCIA Mucor circinelloides from light induced mycelia


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: grown in the dark on several media. The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. The library was constructed and sequencing performed at the DOE Joint Genome Institute (Walnut Creek, CA).

characteristic: lab-host

text: Mucor circinelloides

text: SalI

text: NotI

text: whole mycelium

text: CCIB Mucor circinelloides from dark grown mycelia


text: Federal University of Vicosa

text: live

text: Generic

text: fibroblast

characteristic: cell type

text: These EST sequences were obtained as part of a collaborative project between the Federal University of Vicosa and Embrapa Dairy Cattle Research Center (Brazil). Semimembranosus samples were collected from Duroc, Large White and Piau adult male animals. RNA samples were extracted with Maxi Kit (Qiagen) and the cDNA libraries were constructed with Superscript Plasmid System with Gateway Technology for cDNA Synthesis and Cloning (Invitrogen).

text: adult

text: E.coli

text: Sus scrofa

characteristic: organism

text: NotI

text: SalI

text: male

text: Large White, Duroc, Piau

text: skeletal muscle

text: UFV_EMBRAPA_Brazilian_PIG MUSCLE cDNA_Library

text: ampicilin resistant


text: Swinburne University

text: live

text: Generic

text: Cranbrook

characteristic: cultivar

text: ss-cDNA prepared from RNA extracted from pooled aerial tissues of 8-day-old seedlings

text: Seedling

text: Triticum aestivum

text: Pooled aerial tissues

text: Triticum aestivum 8-day-old unstressed aerial tissues

characteristic: title


characteristic: External Id

text: National Academy of Agricultural Science

text: live

text: Generic

text: E.coli DH5a

text: Sparus aurata

text: EcoRI

text: EcoRI

text: liver

text: Subtractive cDNA library specific for liver of Sparus aurata

text: plasmid

text: pGEM T Easy

characteristic: vector


text: Memorial University of Newfoundland

characteristic: INSDC center name

text: live

text: Generic

text: hepatocyte

text: From Nov. 27th to Dec.17th, 2007, hepatocytes were isolated from individual smelt that had been held at 8oC and were not accumulating glycerol. These cells were split into individual vials containing 5 million cells and were incubated at either 8oC (no glycerol accumulation) or 0oC (glycerol accumulation) for 72h. Total RNA was pooled (0oC pool, 8oC pool) from the 9 fish with the highest levels of glycerol at 0oC and used for constructing forward (cold up-regulated/warm down-regulated) and reverse (cold down-regulated/warm up-regulated) reciprocal SSH cDNA libraries.

text: Osmerus mordax

text: EcoRI

text: EcoRI

text: male

text: liver

characteristic: tissue-type

text: Osmerus mordax hepatocyte SSH library enriched for genes up-regulated by cold-induced glycerol production

text: plasmid

text: pGEM-T easy


text: Memorial University of Newfoundland

characteristic: INSDC first public

text: live

text: Generic

text: hepatocyte

text: Osmerus mordax

text: EcoRI

text: EcoRI

text: male

text: liver

text: Osmerus mordax hepatocyte SSH library enriched for genes down-regulated by cold-induced glycerol production

text: plasmid

text: pGEM-T easy


text: Unversity of Pennsylvania School of Medicine

text: live

text: Generic

characteristic: cell-line

text: 5' RACE using GeneRacer (kit) invitrogen followed by Topo cloning then Sanger sequencing

text: Homo sapiens

text: SVA 5' RACE from 293T cell line


text: live

text: Generic

text: Hela

text: Homo sapiens

text: SVA 5' RACE from Hela cell line


text: live

text: Generic

text: Pan troglodytes

text: Male

characteristic: sex

text: Testes

text: SVA 5' RACE from chimp testes


characteristic: INSDC last update

text: live

text: Generic

text: nTera

text: Homo sapiens

text: SVA 5' RACE from nTera cell line


text: DOE Joint Genome Institute

text: live

characteristic: INSDC status

text: Generic

text: In Vivo Conditions: starvation-induced multicellular development. The library was made from oligo(dT)-primed cDNA and cloned into the pCS107 vector (courtesy of R. Harland, University of California, Berkeley). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT -3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3') and digested with NotI. cDNA was size selected >2.5kb using 1.1% agarose gel electrophoresis, then ligated into NotI- and SalI- digested pCS107 vector. Library was constructed and sequencing performed by the DOE Joint Genome Institute (Walnut Creek, CA).

text: vegetative cells, 12 hr

characteristic: dev-stage

text: Dictyostelium purpureum

text: SalI

text: NotI

characteristic: strain

text: whole organism

text: CBTF Dictyostelium purpureum 12 hr vegetative phase for Dictyostelium discoideum (H)


text: DOE Joint Genome Institute

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: In Vivo Conditions: starvation-induced multicellular development. The library was made from oligo(dT)-primed cDNA and cloned into the pCS107 vector (courtesy of R. Harland, University of California, Berkeley). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT -3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3') and digested with NotI. cDNA was size selected from 0.5-2.5kb using 1.1% agarose gel electrophoresis, then ligated into NotI- and SalI- digested pCS107 vector. Library was constructed and sequencing performed by the DOE Joint Genome Institute (Walnut Creek, CA).

characteristic: description

text: vegetative cells, 12 hr

text: Dictyostelium purpureum

text: SalI

text: NotI

text: whole organism

text: CBTG Dictyostelium purpureum 12 hr vegetative phase for Dictyostelium discoideum (L)


text: DOE Joint Genome Institute

text: live

text: Generic

text: vegetative cells, 6 hr

characteristic: lab-host

text: Dictyostelium purpureum

characteristic: organism

text: SalI

text: NotI

text: whole organism

text: CBXU Dictyostelium purpureum 6 hr vegetative phase for Dictyostelium discoideum (H)


text: DOE Joint Genome Institute

text: live

text: Generic

text: vegetative cells, 6 hr

text: Dictyostelium purpureum

text: SalI

text: NotI

text: whole organism

text: CBXW Dictyostelium purpureum 6 hr vegetative phase for Dictyostelium discoideum (L)

characteristic: title


characteristic: External Id

text: DOE Joint Genome Institute

text: live

text: Generic

text: Dictyostelium purpureum

text: SalI

text: NotI

text: CBZY Dictyostelium purpureum 18 hr starvation induced multicellular development (H)


text: DOE Joint Genome Institute

text: live

text: Generic

text: Dictyostelium purpureum

text: SalI

text: NotI

text: CBZZ Dictyostelium purpureum 18 hr starvation induced multicellular development (L)

characteristic: vector


text: DOE Joint Genome Institute

characteristic: INSDC center name

text: live

text: Generic

text: Dictyostelium purpureum

text: SalI

text: NotI

text: CCAA Dictyostelium purpureum 0 hr starvation induced multicellular development (H)


text: DOE Joint Genome Institute

text: live

text: Generic

text: Dictyostelium purpureum

text: SalI

text: NotI

text: CCAB Dictyostelium purpureum 0 hr starvation induced multicellular development (L)


text: Central Institute Of Brackishwater Aquaculture

text: live

text: Generic

text: adult

text: Penaeus monodon

text: gill

characteristic: tissue-type

text: EST library from normal Indian tiger shrimp Penaeus monodon

text: plasmid

text: pSPORT 1


text: University of Maryland

characteristic: INSDC first public

text: live

text: Generic

text: normalized

text: adult

text: Oreochromis niloticus

text: adult whole brain

text: Tilapia adult whole brain library


text: University of Maryland

text: live

text: Generic

text: normalized

text: 0-4 days post-fertilization

text: Oreochromis niloticus

text: whole embryos

text: Tilapia embryo 0-4 days post-fertilization library


text: University of Maryland

text: live

text: Generic

text: normalized

text: 5-15 days post fertilization

text: Oreochromis niloticus

text: whole larvae

text: Tilapia larvae 5-15 days post-fertilization library


text: University of Maryland

text: live

text: Generic

text: normalized

text: 16-40 days postfertilization

text: Oreochromis niloticus

text: whole juveniles

text: Tilapia juveniles 16-40 days post-fertilization library


text: University of Maryland

text: live

text: Generic

text: not normalized

text: adult

text: Oreochromis niloticus

text: gill

text: Tilapia adult unnormalized gill library


text: University of Maryland

text: live

text: Generic

text: normalized

text: adult

text: Oreochromis niloticus

text: gill

text: Tilapia adult normalized gill library


text: CIRAD-Persyst

text: live

text: Generic

text: XY males, XX females treated at 27oC and XX females treated at 36oC. Pools of gonads were used to prepare total RNAs which were then used for dscDNA with the SMART approach (Evrogen). Normalization was performed using the DSN method. The library was prepared after amplification of the normalized fraction.

text: 14 to 54 days post-fertilization gonads

text: gonads

characteristic: organ

text: Oreochromis niloticus

text: Ivory Coast Bouake strain

text: Tilapia library of mixed differentiating XX and XY gonads


text: University of Maryland

text: live

text: Generic

text: normalized

text: adult

text: Oreochromis niloticus

text: heart

text: Tilapia adult heart library


text: University of Maryland

text: live

text: Generic

text: normalized

text: adult

text: Oreochromis niloticus

text: kidney

text: Tilapia adult kidney library


text: University of Maryland

text: live

text: Generic

text: normalized

text: adult

text: Oreochromis niloticus

text: liver

text: Tilapia adult liver library


text: University of Maryland

text: live

text: Generic

text: normalized

text: adult

text: Oreochromis niloticus

text: olfactory epithelium

text: Tilapia adult olfactory epithelium library


text: University of Maryland

text: live

text: Generic

text: normalized

text: adult

text: Oreochromis niloticus

text: female

characteristic: sex

text: ovary

text: Tilapia adult ovary library


text: University of Maryland

characteristic: INSDC last update

text: live

text: Generic

text: not normalized

text: adult

text: Oreochromis niloticus

text: retina

text: Tilapia adult unnormalized retina and retinal pigment epithelium library


text: University of Maryland

text: live

characteristic: INSDC status

text: Generic

text: normalized

text: adult

text: Oreochromis niloticus

text: retina

text: Tilapia adult normalized retina and retinal pigment epithelium library


text: University of Maryland

text: live

text: Generic

text: normalized

text: adult

characteristic: dev-stage

text: Oreochromis niloticus

text: skin

text: Tilapia adult skin library


text: University of Maryland

text: live

text: Generic

text: normalized

text: adult

text: Oreochromis niloticus

text: skeletal muscle

text: Tilapia adult skeletal muscle library


text: University of Maryland

text: live

text: Generic

text: normalized

text: adult

text: Oreochromis niloticus

text: spleen

text: Tilapia adult spleen library


text: University of Maryland

text: live

text: Generic

text: normalized

text: adult

text: Oreochromis niloticus

text: stomach

text: Tilapia adult stomach library


text: University of Maryland

text: live

text: Generic

text: normalized

text: adult

text: Oreochromis niloticus

text: male

text: testis

text: Tilapia adult testis library


text: Reliance Life Sciences Pvt Ltd

text: live

text: Generic

text: Young 3-4 week old in vitro germinated Jatropha curcas were removed from the media, following which they were randomly separated into groups of 15-20 plantlets. Groups of these plants were placed in 150mM NaCl solution for 1.5-2.0 hours.Total RNA for cDNA synthesis was extracted from Jatropha curcas root samples using Qiagen RNA Miniprep Kit (Qiagen, Germany). Subsequent steps of cDNA synthesis and library construction were performed using Super SMART cDNA synthesis kit (Clontech).

text: 3-4 week old in vitro germinated plantlets

text: Jatropha curcas

text: Pvu II

text: BstE II

characteristic: strain

text: Root

text: Salinity tolerance responsive genes from Jatropha curcas root cDNA

text: Plasmid

text: pYES2.1 TOPO TA cloning vector


text: live

text: Generic

characteristic: NCBI submission model

text: adult

text: XL-1 BLUE

text: Penaeus monodon

text: XbaI

text: BamHI

text: whole animal

text: RACE PCR Amplified Penaeus monodon cDNA Library

text: plasmid


text: Institute of Crop Science Chinese Academy of Agricultural Sciences

text: live

text: Generic

characteristic: NCBI submission package

text: The seedlings at jointing stage were treated with PEG-6000 used as the 'tester' sample, the untreated were used as the control 'driver' sample. Total RNA was extracted by Trizol from leaves, then reverse transcription was conducted by AMV reverse transcriptase; doublestrand cDNA was synthesized and subjected to SSH analysis by using PCR Select cDNA Subtraction Kit.

text: jointing

text: Triticum aestivum

text: leaf

text: Triticum aestivum at jointing stage


text: University of Minnesota Duluth

text: live

text: Generic

text: Rapid cycling, CrGC 1-33

characteristic: cultivar

text: Lateral nectaries were manually dissected from open B. rapa flowers (equivalent of Stage 14-15 in Arabidopsis (Smyth et al. 1990)) and pooled in separate tubes containing RNAlater solution (Ambion, Austin, TX) on ice, and stored at -20 C prior to RNA extraction. Individual samples containing ca. 250 nectaries were processed for RNA isolation with Stratagene's Absolutely RNA Miniprep Kit (#400800). Total RNA quality was assessed by standard UV spectrophotometry and agarose gel electrophoresis; individual preparations yielded ~10 micrograms of total RNA. A non-normalized library was generated from 1 microgram of total RNA with the Creator SMART cDNA Library Construction Kit (Clontech #634903) according to the manufacturers directions (LD PCR method). All cDNA fragments were ligated into the SfiI A and SfiI B sites of pDNR-LIB, and transformed into DH5a.

characteristic: description

text: Adult

text: E. coli (DH5a)

text: Nectary

text: Brassica rapa

text: SfiI

text: SfiI

text: Brassica rapa mature lateral nectary, non-normalized (MLN1)

text: plasmid

text: pDNR-LIB


text: University of Minnesota Duluth

text: live

text: Generic

text: Rapid cycling, CrGC 1-33

text: Lateral nectaries were manually dissected from open B. rapa flowers (equivalent of Stage 14-15 in Arabidopsis (Smyth et al. 1990)) and pooled in separate tubes containing RNAlater solution (Ambion, Austin, TX) on ice, and stored at -20 C prior to RNA extraction. Individual samples containing ca. 250 nectaries were processed for RNA isolation with Stratagene's Absolutely RNA Miniprep Kit (#400800). Total RNA quality was assessed by standard UV spectrophotometry and agarose gel electrophoresis; individual preparations yielded ~10 micrograms of total RNA. A normalized library was prepared by Creative Genomics, Corp. (Port Jefferson Station, New York) from 15 micrograms of total RNA by first amplifying cDNA with the Creator SMART cDNA Library Construction Kit, and then normalizing the product with the Trimmer-direct Kit (Evrogen #NK002). All cDNA fragments were ligated into the SfiI A and SfiI B sites of pDNR-LIB, and transformed into DH10B.

text: Adult

text: E. coli (DH10B)

characteristic: lab-host

text: Nectary

text: Brassica rapa

characteristic: organism

text: SfiI

text: SfiI

text: Brassica rapa mature lateral nectary, normalized (MLN2)

text: plasmid

text: pDNR-LIB


characteristic: External Id

text: University of Minnesota Duluth

text: live

text: Generic

text: Rapid cycling, CrGC 1-33

text: Median nectaries were manually dissected from open B. rapa flowers (equivalent of Stage 14-15 in Arabidopsis (Smyth et al. 1990)) and pooled in separate tubes containing RNAlater solution (Ambion, Austin, TX) on ice, and stored at -20 C prior to RNA extraction. Individual samples containing ca. 250 nectaries were processed for RNA isolation with Stratagene's Absolutely RNA Miniprep Kit (#400800). Total RNA quality was assessed by standard UV spectrophotometry and agarose gel electrophoresis; individual preparations yielded ~10 micrograms of total RNA. A non-normalized library was generated from 1 microgram of total RNA with the Creator SMART cDNA Library Construction Kit (Clontech #634903) according to the manufacturers directions (LD PCR method). All cDNA fragments were ligated into the SfiI A and SfiI B sites of pDNR-LIB, and transformed into DH5a.

text: Adult

text: E. coli (DH5a)

text: Nectary

text: Brassica rapa

text: SfiI

text: SfiI

text: Brassica rapa mature median nectary, non-normalized (MMN1)

characteristic: title

text: plasmid

text: pDNR-LIB


text: University of Minnesota Duluth

characteristic: INSDC center name

text: live

text: Generic

text: Rapid cycling, CrGC 1-33

text: Median nectaries were manually dissected from open B. rapa flowers (equivalent of Stage 14-15 in Arabidopsis (Smyth et al. 1990)) and pooled in separate tubes containing RNAlater solution (Ambion, Austin, TX) on ice, and stored at -20 C prior to RNA extraction. Individual samples containing ca. 250 nectaries were processed for RNA isolation with Stratagene's Absolutely RNA Miniprep Kit (#400800). Total RNA quality was assessed by standard UV spectrophotometry and agarose gel electrophoresis; individual preparations yielded ~10 mg of total RNA. A normalized library was prepared by Creative Genomics, Corp. (Port Jefferson Station, New York) from 15 micrograms of total RNA by first amplifying cDNA with the Creator SMART cDNA Library Construction Kit, and then normalizing the product with the Trimmer-direct Kit (Evrogen #NK002). All cDNA fragments were ligated into the SfiI A and SfiI B sites of pDNR-LIB, and transformed into DH10B.

text: Adult

text: E. coli (DH10B)

text: Nectary

text: Brassica rapa

text: SfiI

text: SfiI

text: Brassica rapa mature median nectary, normalized (MMN2)

text: plasmid

text: pDNR-LIB

characteristic: vector


text: DOE Joint Genome Institute

characteristic: INSDC first public

text: live

text: Generic

text: The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected >2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed and sequencing performed at the DOE Joint Genome Institute (Walnut Creek, CA).

text: Heterobasidion annosum

text: SalI

text: NotI

text: TC 32-1

text: CCOZ Heterobasidion annosum Liquid Hagem-medium (H)


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 0.6-2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed and sequencing performed at the DOE Joint Genome Institute (Walnut Creek, CA).

text: Heterobasidion annosum

text: SalI

text: NotI

text: TC 32-1

text: CCPA Heterobasidion annosum Liquid Hagem-medium (L)


text: DOE Joint Genome Institute

text: live

text: Generic

text: Heterobasidion annosum

text: SalI

text: NotI

text: TC 32-1

text: CCPB Heterobasidion annosum Liquid high nitrogen MMN-medium (H)


text: DOE Joint Genome Institute

text: live

text: Generic

text: Heterobasidion annosum

text: SalI

text: NotI

text: TC 32-1

text: CCPC Heterobasidion annosum Liquid high nitrogen MMN-medium (L)


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: normal light conditions (15 umolphoton/m2/sec). The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected >2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed at DOE Joint Genome Institute (Walnut Creek, CA).

text: exponentional growth stage

text: Ostreococcus sp. RCC809

text: SalI

text: NotI

text: whole cells (unicellular organism)

characteristic: tissue-type

text: CBPU Ostreococcus sp. RCC809 normal conditions (H)


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: normal light conditions + 2 hours high light (800 umol/m2/sec) just before RNA extraction. The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected >2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed at DOE Joint Genome Institute (Walnut Creek, CA).

text: exponentional growth stage

text: Ostreococcus sp. RCC809

text: SalI

text: NotI

text: whole cells (unicellular organism)

text: CBPX Ostreococcus sp. RCC809 high light conditions (H)


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: normal light conditions + 2 hours high light (800 umol/m2/sec) just before RNA extraction. The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 1.2-2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed at DOE Joint Genome Institute (Walnut Creek, CA).

text: exponentional growth stage

text: Ostreococcus sp. RCC809

text: SalI

text: NotI

text: whole cells (unicellular organism)

text: CBPY Ostreococcus sp. RCC809 high light conditions (L)


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: dark condition. The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected >0.5 kb using 1.1% agarose gel electrophoresis, then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed and sequencing performed at the DOE Joint Genome Institute (Walnut Creek, CA).

text: Glycine max

text: SalI

text: NotI

text: Williams 82

text: callus

text: CCCC Glycine max callus grown in dark condition


text: ICBIBE, Universitat de Valencia

text: live

text: Generic

text: Suppression Subtractive Hybridization library using cDNA derived from L3 nymphs from G1 generation reared at 15oC in short day conditions (12L:12D) as tester and cDNA derived from L3 aphids reared at long day conditions (16L:8D) as driver. The name of the library estands for Short Day Upregulated genes (SDU).

text: nymphs L3 from G1 generation

text: Acyrthosiphon pisum

text: parthenogenetic females

characteristic: sex

text: whole insect

text: plasmid

text: pGEM-T Easy


text: ICBIBE, Universitat de Valencia

characteristic: INSDC last update

text: live

text: Generic

text: Suppression Subtractive Hybridization library using cDNA derived from L3 nymphs from G1 generation reared at 15oC in long day conditions (16L:8D) as tester and cDNA derived from L3 aphids reared at short day conditions (12L:12D) as driver. The name of the library estands for Short Day Downregulated genes (SDD).

text: nymphs L3 from G1 generation

text: Acyrthosiphon pisum

text: parthenogenetic females

text: whole insect

text: plasmid

text: pGEM-T Easy


text: ICBIBE, Universitat de Valencia

text: live

characteristic: INSDC status

text: Generic

text: nymphs L3 from G1 generation

text: Acyrthosiphon pisum

text: parthenogenetic females

text: GV

text: whole insect

text: plasmid

text: pGEM-T Easy


text: ICBIBE, Universitat de Valencia

text: live

text: Generic

text: nymphs L3 from G1 generation

characteristic: dev-stage

text: Acyrthosiphon pisum

text: parthenogenetic females

text: GV

text: whole insect

text: plasmid

text: pGEM-T Easy


text: British Antarctic Survey

text: live

text: Generic

text: directional cDNA from Cryptopygus antarcticus with a low super-cooling point

text: Cryptopygus antarcticus

text: whole

text: pooled low supercooling point Cryptopygus antarcticus

text: plasmid

text: pDNR-lib


text: British Antarctic Survey

text: live

text: Generic

text: directional cDNA from Cryptopygus antarcticus with a high super-cooling point

text: Cryptopygus antarcticus

text: whole

text: pooled high supercooling point Cryptopygus antarcticus

text: plasmid

text: pDNR-lib


text: British Antarctic Survey

text: live

text: Generic

text: non-directional cDNA from pooled Cryptopygus antarcticus under control conditions

text: Cryptopygus antarcticus

text: whole

text: pooled control Cryptopygus antarcticus

text: plasmid

text: pGEM-teasy


text: The Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences

text: live

text: Generic

text: adult

text: Panax quinquefolius

text: Flower

text: American ginseng Flower cDNA Library

text: Plasmid

text: pBluescriptII


text: live

text: Generic

text: adult

text: Panax quinquefolius

text: Leaf

text: American ginseng Leaf cDNA Library

text: Plasmid

text: pDNR-LIB


text: live

text: Generic

text: adult

text: Panax quinquefolius

text: Root

text: American ginseng Root cDNA Library

text: Plasmid

text: pBluescriptII


text: The Samuel Roberts Noble Foundation

text: live

text: Generic

text: Alamo

text: The vascular bundle specific cDNA libraries were constructed from total RNA isolated from vascular tissues of the leaf sheath and second internode of switchgrass. The library was constructed in the pDNR-LIB vector. First-strand cDNA was constructed using MMLV Reverse Transcriptase and amplified by PCR using 5' PCR primers and CDS III/3' PCR primer. The resulting cDNA was digested with Sfil enzyme and ligated into pDNR-LIB and transformed into Eletromax DH10B E. coli cells.

text: stem and leaf

characteristic: organ

text: Panicum virgatum

text: vascular tissue

text: cDNA library of switchgrass vascular tissue derived by LCM

text: cloning vector for cDNA library construction

text: pDNR-LIB vector


text: Faculty of Agriculture and Natural Science, University of Pretoria

text: live

text: Generic

characteristic: NCBI submission model

text: Betta NILs

text: 3-4 leaf stage

text: Triticum aestivum

text: Leaf

text: Wheat Betta NILs RWA induced cDNA library

text: Plasmid #K1214


text: National University of Ireland Maynooth

text: live

text: Generic

characteristic: NCBI submission package

text: cDNA was synthesized from total RNA isolated from mixed stage unstressed nematodes and the cDNA library was constructed using a Creator SMART cDNA Library Construction Kit (Clontech).

text: Mixed stage population

text: E. coli XL1-Blue

text: Panagrolaimus superbus

characteristic: strain

text: Unstressed mixed stage Panagrolaimus superbus nematodes

text: pDNR-LIB (Clontech)


text: School of Life Sciences, Lanzhou University, China

text: live

text: Generic

text: cDNA-AFLP

characteristic: description

text: Rheum nobile

text: hermaphrodite

text: Bracts and Leaves

text: Bracts and Leaves of Rheum nobile Library


text: Israel Oceanographic and Limnological Research

text: live

text: Generic

text: The library was prepared using the SSH (Subtractive-Suppression Hybridization) method aimed at T-A cloning of a roughly equally represented cDNAs from the target organs into an expression plasmid (pGEM-Teasy, Promega) biased towards genes differentially expressed in the target tissues in comparison to the reference one.

text: Adult

text: Hepatopancreas, hypodermis, gastrolith disk

text: Cherax quadricarinatus

text: male

text: Premolt crayfish library

text: Plasmid

text: pGEM-T easy


text: Central Institute of Freshwater Aquaculture

text: live

text: Generic

text: RNA was isolated from the pallial mantle of both the implanted and non-implanted pearl mussel L.marginalis. Tester and driver cDNA were synthesized from the respective implanted and non-implanted RNA. The SSH library was made in TOPO TA cloning vector of Invitrogen and sequenced in ABI-3130 Genetic Analyzers.

text: E.coli

characteristic: lab-host

text: Lamellidens marginalis

text: pallial mantle

text: SSH library of mantle in freshwater pearl mussel L.marginalis

text: plasmid

text: TOPO TA (Invitrogen)


text: National Center for Cell Science

text: live

text: Generic

characteristic: cell type

text: Aedes albopictus

characteristic: organism

text: female

text: Cell line

text: pGEMT


characteristic: External Id

text: INSERM

text: live

text: Generic

text: Complementary DNAs were synthesized and amplified from total RNA of left or right ovaries, using the SMART PCR cDNA synthesis kit (BD Biosciences Clontech). The suppression subtractive hybridization (SSH) screening was performed with PCR-Select cDNA subtraction kit (BD Biosciences Clontech), using left ovary cDNAs as tester and right ovary cDNAs as driver.

text: 8-day embryo

text: left ovary

text: Gallus gallus

text: female

text: White Leghorn

text: Chicken 8-day embryonic left ovary SSH library

characteristic: title

text: pGEMT-easy


text: Japan Tobacco Inc.

characteristic: INSDC center name

text: live

text: Generic

text: mixture of 11 days after transplanting, 24 days to 48 days after transplanting

text: Nicotiana tabacum

text: mixture of leaves, apical buds, roots

text: normalized full-length tobacco cDNA library


text: Institute of Himalyan Bioresource Technology (CSIR)

characteristic: INSDC first public

text: live

text: Generic

text: Arnebia euchroma

text: Callus

text: differentially expressed ESTs in response to colour production

text: pGEM-T Vector (Promega)

characteristic: vector


text: University of Bari, Faculty of Agriculture

text: live

text: Generic

text: Golden Delicious

characteristic: cultivar

text: Escherichia coli DH5a

text: Malus domestica

text: Fruit

characteristic: tissue-type

text: Apple Induced Resistance (AIR)

text: pCR II


text: China Agricultural University

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Azospirillum brasilense

text: Azospirillum brasilense Sp7 cDNA-AFLP fragment


text: Moscow State University

text: live

text: Generic

text: Nicotiana benthamiana

text: leaf

text: Nicotiana benthamiana leaf, Methanol-mediated defensive reactions


text: National Institute of Allergy and Infectious Diseases

text: live

text: Generic

text: Unidirectional library was constructed using long distance PCR (Smart cDNA kit) and Lambda TriplEx2 vector

text: adult female

characteristic: dev-stage

text: salivary glands

characteristic: organ

text: Culex quinquefasciatus

text: female

characteristic: sex

text: Adult female salivary gland cDNA library CquiSG1

text: Phage

text: Lambda TriplEx2


text: Colorado State University

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Library made from RNA collected from ovaries dissected from 50 4-day old adult female Aedes albopictus, 3 day post blood feed.

text: adult

text: ovary

text: Aedes albopictus

text: female

text: Lake Charles

characteristic: strain

text: Aedes albopictus Ovary 3 day post bloodfeed


text: National Institute of Animal Science (Korea)

text: live

text: Generic

text: 20 months old

characteristic: lab-host

text: Brain

text: Canis lupus familiaris

text: two males and two females mixed

text: Brain cerebral cortex

text: Jindo brain cDNA library


text: live

text: Generic

text: 20 months old

text: Liver

text: Canis lupus familiaris

characteristic: organism

text: two males and two females mixed

text: Liver right lobe

text: Jindo liver cDNA library


characteristic: External Id

text: live

text: Generic

characteristic: description

text: adult female

text: salivary glands

text: Hyalomma rufipes

text: female

text: Collected off cows in Point G, Bamako, Mali

text: Adult female salivary gland cDNA library HyalmargSG1

text: Phage

text: Lambda TriplEx2


characteristic: INSDC center name

text: live

text: Generic

text: adult

text: salivary glands

text: Cimex lectularius

text: Bed bug salivary gland cDNA library Cilecsg1

characteristic: title

text: Phage

text: Lambda TriplEx2


text: Central Institute of Freshwater Aquaculture

characteristic: INSDC first public

text: live

text: Generic

text: A testis cDNA library of Labeo rohita (rohu) generated by SSH technique.

text: Matured

text: E.coli

text: Labeo rohita

text: Male

text: Testis

text: Rohu testis library

text: Plasmid

text: pGEM-T easy vector

characteristic: vector


text: CRA - FSO

text: live

text: Generic

text: Adult

text: E. coli

text: Leaf

text: Kalanchoe x houghtonii

text: Leaf margin

characteristic: tissue-type

text: Kalanchoe x houghtonii SSH cDNA library

text: Plasmidic vector

text: pGEMtEasy vector


text: IASMA Research and Innovation Centre, Fondazione Edmund Mach

characteristic: INSDC last update

text: live

text: Generic

text: Kober 5BB rootstock

characteristic: cultivar

text: Young roots of Kober 5BB plants were sandwiched between dishes containing confluent mycelia of Armillaria mellea 7A (tester). As control (driver), the root systems of other plants were sandwiched between two uninoculated dishes. The forward subtracted SSH cDNA library (enriched with A. mellea-induced cDNAs) was obtained according to PCR-Select cDNA Subtraction kit (Clontech).

text: 2 months-old Kober 5BB rooted cuttings, 24 h after Armillaria mellea treatment

text: Vitis cinerea var. helleri x Vitis riparia

text: roots

text: SSH library of A. mellea treated grapevine roots

text: Plasmid

text: pCR2.1 TOPO vector (Invitrogen)


text: National Institute of Plant Genome Research

text: live

characteristic: INSDC status

text: Generic

text: Genotype specific sustracted cDNA library from uninfected vs Fusarium oxysporum challanged Chickpea (Cicer arietinum L.) cultivar JG-62.

text: Seedling

characteristic: dev-stage

text: Cicer arietinum

text: Root and collar region

text: Early immune responsive suppression subtracted cDNA libraries of Chickpea (Cicer arietinum cv. JG-62) wilt

text: pGEMT


text: live

text: Generic

text: Genotype specific sustracted cDNA library from uninfected vs Fusarium oxysporum challanged Chickpea (Cicer arietinum L.) cultivar WR-315.

text: Seedling

text: Cicer arietinum

text: Root and collar region

text: Early immune responsive suppression subtracted cDNA libraries of Chickpea (Cicer arietinum cv. WR-315) wilt

text: pGEMT


text: M.M. Shemyakin & Yu.A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences

text: live

text: Generic

text: brain

characteristic: organ

text: Coregonus migratorius

text: Baikal omul brain cDNA Library


text: live

text: Generic

characteristic: NCBI submission model

text: brain

text: Coregonus baicalensis

text: Baikal whitefish brain cDNA Library


text: Max Planck Institute for Chemical Ecology

text: live

text: Generic

characteristic: NCBI submission package

text: Manduca midgut transcriptome analysis

text: digestive tract

text: Manduca sexta

text: Male and Female

characteristic: sex

text: midgut

text: Msex gut

text: Plasmid

text: pDNR-Lib


text: Universidade Estadual de Santa Cruz

text: live

text: Generic

text: For the cDNA library construction, the M. perniciosa strain CP03 (CEPEC 1108) was cultured as previously described and mycelium samples in white, yellow, reddish-pink, dark reddish pink and primordium stages, as well as from basidiomata were used to construct a full-length, non-normalized cDNA library. Total RNA was extracted from samples using RNAs in RNA Plant Midi Kit as described by the manufacturer (Qiagen) and after quantification, 1 ug was used to construct the library using DB SMART Creator cDNA library as described by the manufacturer (Clontech). cDNA strands longer than 400 bp were cloned directionally into the pDNR-LIB plasmid. ElectroMAXTM DH10BTM cells (Invitrogen) were transformed and colonies selected and grown in 96-well microtiter plates in LB, 40% glycerol medium containing 30 ug/L chloramphenicol and stored at -80o C.

text: fruiting

text: CP 03 (CEPEC 1108)

characteristic: isolate

text: Moniliophthora perniciosa

text: mycelia and basidiomata

text: EB - mycelia growing on artificial medium to induce basidiomata production

text: Plasmid

text: pDNR-LIB


text: Max Planck Institute for Plant Breeding Research

text: live

text: Generic

text: Colletotrichum higginsianum

characteristic: organism

text: IMI 349063A

characteristic: strain

text: Colletotrichum higginsianum biotrophic hyphae


characteristic: External Id

text: National Institute for Basic Biology

text: live

text: Generic

text: adult

text: Oryzias latipes

text: male

text: Hd-rR

text: testis

text: OLTE


text: Departement de Biochimie, Universite de Montreal

characteristic: INSDC center name

text: live

text: Generic

text: host: Zootermopsis angusticollis

characteristic: description

text: termite gut metagenome

text: Metatranscriptome of hindgut from Zootermopsis angusticollis Non-normalized


text: Central Institute of Medicinal and Aromatic Plants (CIMAP, CSIR)

characteristic: INSDC first public

text: live

text: Generic

text: CIM Poshita

text: Poly (A)+ RNA isolated from the leaves of eighteen week old Withania somnifera (cv. CIM Poshita) plants growing in the CIMAP farm at Lucknow under the National Gene Bank for Medicinal and Aromatic Plants, was used for preparing the cDNA library in Lambda Zap Express vector (Stratagene).

text: Eighteen week old field-growing plant

text: Leaf

text: Withania somnifera

text: Withania somnifera leaf cDNA library in Lambda Zap Express vector

characteristic: title

text: Phagemid

text: pBK-CMV


text: live

text: Generic

text: CIM Poshita

text: Poly (A)+ RNA isolated from the roots of eighteen week old Withania somnifera (cv. CIM Poshita) plants growing in the CIMAP farm at Lucknow under the National Gene Bank for Medicinal and Aromatic Plants, was used for preparing the cDNA library in Lambda Zap Express vector (Stratagene).

text: Eighteen week old field-growing plant

text: Root

text: Withania somnifera

text: Withania somnifera root cDNA library in Lambda Zap Express vector

text: Phagemid

text: pBK-CMV

characteristic: vector


text: Huazhong Agricultural University

characteristic: INSDC last update

text: live

text: Generic

text: Gossypium barbadense

text: root

characteristic: tissue-type

text: 7124 root normalized cDNA library


text: Jilin university

text: live

characteristic: INSDC status

text: Generic

text: We used the representational difference analysis (RDA) method to construct the subtractive libraries of cDNA of the A. pleuropneumoniae strains CVCC259 (serotype 1) and CVCC261 (serotype 3).ESTs of CVCC259 were obtained by using strain CVCC259 as the tester and strain CVCC261 as the driver.

text: Logarithmic growth phase

text: Actinobacillus pleuropneumoniae

text: Actinobacillus pleuropneumoniae strain CVCC259 subtractive library


text: Jilin university

text: live

text: Generic

text: We used the representational difference analysis (RDA) method to construct the subtractive libraries of cDNA of the A. pleuropneumoniae strains CVCC259 (serotype 1) and CVCC261 (serotype 3).ESTs of CVCC261 were obtained by using strain CVCC261 as the tester and strain CVCC259 as the driver.

text: Logarithmic growth phase

characteristic: dev-stage

text: Actinobacillus pleuropneumoniae

text: Actinobacillus pleuropneumoniae strain CVCC261 subtractive library


text: Key Laboratory of Tropic Biological Resources of Ministry of Education Hainan University

text: live

text: Generic

text: SSH and RNA dot blotting were used to identify genes differential expression in mesocarp and kernel of oil palm nut.

text: Elaeis guineensis

text: mesocarp and kernel of oil palm nut

text: oil palm nut mesocarp and kernel differential expression


text: National Institute for Genetic Engineering and Biotechnology

text: live

text: Generic

text: Twenty_one_day old Aeluropus lagopoides seedlings were exposed to 600 mM NaCl for 10 days prior to total RNA extraction

text: 8th leaf

characteristic: lab-host

text: Shoot

characteristic: organ

text: Aeluropus lagopoides

text: HindIII

text: EcoRI

text: Leaf

text: High-salt treated Aeluropus lagopoides library

text: Plasmid


text: EBC, Uppsala University

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: hemocyte

characteristic: cell type

text: Pacifastacus leniusculus

text: male

text: Plhc, Pacifastacus leniusculus Lambda Zap Express Library HPT cDNA


text: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences

text: live

text: Generic

text: sporozoites were obtained from sporulated oocysts. sporulated oocysts were obtained from cecea of of E.tenella infected chickens. sporozoites subtracted cDNA library using cDNA from sporozoites as testers and cDNA from sporulated oocysts as drivers. Subtractive PCRs were performed using the PCR-select cDNA subtractive kit according to the manufacture's protocol (Clontech). PCR products then were cloned into the pGEM-T Easy vector (Promega) and transformed into JM109.

text: sporozoites

text: Eimeria tenella

characteristic: organism

text: Eimeria tenella sporozoites subtracted cDNA library

text: pGEM-T-easy Vector(Promega)


text: Dana-Farber Cancer Institute

text: live

text: Generic

text: pDONR223 is a Gateway Vector

text: Homo sapiens

text: human reverse-transcribed RNA

text: plasmid


text: ARC-Roodeplaat Vegetable and Ornamental Plant institute

text: live

text: Generic

text: IT96D-602 and Tvu7778

characteristic: cultivar

text: Total RNA was isolated from leaf samples using Tri-Reagent (Sigma) and Plant RNA isolation aid (Ambion). Contaminating genomic DNA was removed with the Turbo DNA-free kit (Ambion) and the RNA cleaned up with the Plant RNeasy kit (Qiagen). Equal quantities of total RNA from 9 and 12 days of drought stressed IT96D-602 and control Tvu7778 were pooled to give the tester and driver RNA populations, respectively. mRNA was isolated from 50ug pools of total RNA using an Oligotex mRNA purification kit (Qiagen). Double stranded cDNA was synthesised from mRNA using the cDNA synthesis system (Roche Diagnostics). RsaI-digested cDNA fragments were subtracted in both directions using the PCR-select cDNA subtraction kit (BD Biosciences, Clontech). cDNA enriched for differentially expressed transcripts was cloned into pGEMT-Easy, and transformed into E. coli JM109 cells.

text: Six week old plants

text: E. coli JM109

text: Vigna unguiculata

text: Leaves

text: Cowpea drought expression cDNA library

text: pGEMT-Easy


characteristic: External Id

text: Shanghai Ocean University

text: live

text: Generic

text: Ctenopharyngodon idella

text: Grass carp normalized cDNA library from head kidney induced with Aeromonas hydrophila

text: Plasmid

text: pGEM-T vector


text: Liverpool School of Tropical Medicine

characteristic: INSDC center name

text: live

text: Generic

text: cDNA library created from pooled venom glands isolated from 10 specimens of Echis coloratus

text: Echis coloratus

text: BSRGI

text: BSRGI

text: pool of 10 animals, random age and gender

characteristic: sex

text: Egypt

characteristic: strain

text: Venom Gland

text: Echis coloratus Venom Gland Gateway cDNA library

text: Plasmid

text: Gateway Cloneminer pDONR222


text: Liverpool School of Tropical Medicine

characteristic: INSDC first public

text: live

text: Generic

text: cDNA library created from pooled venom glands isolated from 10 specimens of Echis carinatus sochureki

characteristic: description

text: Echis carinatus sochureki

text: BSRGI

text: BSRGI

text: Sharjah - United Arab Emirates

text: Venom Gland

text: Echis carinatus sochureki Venom Gland Gateway cDNA library

characteristic: title

text: Plasmid

text: Gateway Cloneminer pDONR222


text: Liverpool School of Tropical Medicine

characteristic: INSDC last update

text: live

text: Generic

text: cDNA library created from pooled venom glands isolated from 10 specimens of Echis pyramudim leakeyi

text: Echis pyramidum leakeyi

text: BSRGI

text: BSRGI

text: Lake Baringo - Kenya

text: Venom Gland

characteristic: tissue-type

text: Echis pyramidum leakeyi Venom Gland Gateway cDNA library

text: Plasmid

text: Gateway Cloneminer pDONR222

characteristic: vector


text: Universidade Federal de Uberlandia

text: live

characteristic: INSDC status

text: Generic

text: An endemic species of Brazilian savannah. All the individuals used were obtained by collection in the field.

text: early maturation fruit

characteristic: dev-stage

text: endocarp fruit

text: Caryocar brasiliense

text: endocarp fruit

text: Caryocar brasiliense early maturation fruit


text: Center of Marine Biotechnology, University of Maryland Biotechnology Institute

text: live

text: Generic

characteristic: NCBI submission model

text: Trophozoite

text: Trophozoites propagated in medium [DME-Hams (1-2) 5% FBS] supplemented with Crassostrea virginica oyster serum (50%)

text: Trophozoite

text: Perkinsus marinus

text: TXsc (ATCC 50983)

text: PmS(+)

text: Plasmid

text: pBluescriptKS(-), Lambda ZAP library


text: live

text: Generic

characteristic: NCBI submission package

text: Trophozoite

text: Trophozoites propagated in medium [DME-Hams (1-2) 5% FBS]

text: Trophozoite

text: Perkinsus marinus

text: TXsc (ATCC 50983)

text: PmS(-)

text: Plasmid

text: pBluescriptKS(-), Lambda ZAP library


text: Universidade Federal de Uberlandia

text: live

text: Generic

text: three days after venom extraction

text: venom gland

characteristic: organ

text: Bothrops pauloensis

text: male

text: gland

text: Bothrops pauloensis snake venom gland transcriptome

text: Plasmid

text: pDONRTm 222


text: National Bureau of Fish Genetic Resources

text: live

text: Generic

text: Spleen tissues were collected from acclimatized C. batrachus. Total RNA was extracted followed by mRNA isolation. First strand cDNA was prepared using the CDS-3M adaptor, included in the TRIMMER-DIRECT kit (Evrogen, Moscow, Russia). cDNA was amplified by LD-PCR according to the Creator SMART cDNA Library construction kit (Clontech) protocol and normalized according to TRIMMER-DIRECT protocol (Evrogen). After digestion with SfiI, cDNA were size selected (>500bp) and ligated into the SfiI A and SfiI B sites of pDNR-LIB, and transformed into DH10B.

text: Adult

text: E. coli (DH 10B)

characteristic: lab-host

text: Spleen

text: Clarias batrachus

characteristic: organism

text: Sfi IA

text: Sfi IB

text: Normalized Clarias batrachus spleen cDNA library

text: Plasmid

text: pDNR-Lib


characteristic: External Id

text: IFM-Geomar

text: live

text: Generic

text: country: Germany:Schleswig-Holstein

text: Zostera marina

text: leaf

text: Zostera marina leaf


text: University of Amsterdam

characteristic: INSDC center name

text: live

text: Generic

text: Full-length enriched, normalized cDNA libraries were generated using a combination of the SMART cDNA library construction kit (Clontech) and the Trimmer-Direct cDNA normalization kit (Evrogen), generally following the manufacturer's protocol but with several important modifications. In brief, 2 ug of poly(A)+ mRNA was used for the cDNA library generated and reverse transcription was performed with a mixture of several reverse transcription enzymes (ArrayScript, Ambion; BioScript, Bioline; PrimeScript, TaKaRa; SuperScript II, Invitrogen) for 1h at 42 oC and 90 minutes at 50 oC. cDNA size fractionation was performed with SizeSep 400 spun columns (GE Healthcare) that resulted in a cutoff at ~200 bp. The full-length-enriched cDNAs were cut with SfiI and ligated to pDNR-Lib plasmid (Clontech). Ligations were transformed into E. coli ELECTROMAX DH5a-E electro-competent cells (Invitrogen).

text: Leaf

text: Tarenaya spinosa

text: hermaphrodite

text: Leaf

text: Cleome spinosa normalized leaf ESTs ES1018

text: plasmid

text: pDNR-Lib


text: Florida Institute of Technology

text: live

text: Generic

text: red blood cell

characteristic: cell type

text: Opsanus beta

text: Opsanus Beta Red Blood Cell cDNA Library


text: College of Horticulture, Northwest A&F University

text: live

text: Generic

text: Zaosu

characteristic: cultivar

text: Total RNA was extracted from leaves harvested 0.5, 1, 2, 3, 5, 7 and 9 days after inoculation with Venturia nashicola. The PolyATtract mRNA Isolation System III (Promega) was employed to isolate mRNA. Substractive Hybridization was conducted with the PCR-Select cDNA Subtraction Kit (Clontech). The SSH-cDNA fragments were sub-cloned into the pGEM-T easy vector (Promega) and transformed into E.coli Top10 cells.

text: 0.5, 1, 2, 3, 5, 7 and 9 days after inoculated with Venturia nashicola

text: E. coli TOP 10

text: leaves

text: Pyrus x bretschneideri

text: Rsa I

text: SSH cDNA library of pear cv. Zaosu inoculated with Venturia nashicola

text: plasmid

text: pGEM-T Easy


text: Central Institute of Freshwater Aquaculture

characteristic: INSDC first public

text: live

text: Generic

text: 1st strand cDNA was sythesized from total RNA of Rohu liver by SMART technique using power script reverse transcriptase of Clonetech and CDS-3M adapter of Evrogen.The cDNA was amplified by advantage-2 polymerase mix of Clonetech. The resulting ds-cDNA was normalized according to Trimmer-Direct cDNA normalization procedure of Evrogen. The cDNA were clonned in TOPO TA cloning vector of Invitrogen and sequenced in ABI-3130 Genetic Analyzers.

text: E.coli

text: Labeo rohita

text: liver

text: Rohu liver library

text: plasmid

text: TOPO TA (Invitrogen)


text: National Institute of Advanced Industrial Science and Technology (AIST)

text: live

text: Generic

text: Moesziomyces antarcticus

text: Pseudozyma antarctica EST library


text: Gulbarga University

text: live

text: Generic

text: pUC18/EcoRI cDNA library was constructed from Radicle tissues of 7 day old Pigeon pea seedlings of WRP-1 genotype challenged with 150mM NaCl stress. The cDNA fragments were cloned into pUC18 vector and subsequently sequenced by Sanger sequencing approach using M13 primers.

characteristic: description

text: One week old seedling stage

text: E.Coli

text: Cajanus cajan

text: EcoRI

text: Radicle Tissue

text: Salinity stress induced (7 Days) Pigeon pea genotype WRP-1 pUC18/EcoRI

text: Plasmid


text: Gulbarga University

text: live

text: Generic

text: pGEM-T/EcoRI cDNA library was constructed from Radicle tissues of 7 day old Pigeon pea seedlings of WRP-1 genotype challenged with 150mM NaCl stress. The cDNA fragments were cloned into pGEM-T Easy vector and subsequently sequenced by Sanger sequencing approach using M13 primers.

text: One week old seedling stage

text: E.Coli

text: Cajanus cajan

text: EcoRI

text: Radicle Tissue

text: Salinity stress induced (7 Days) Pigeon pea genotype WRP-1 pGEM-T

characteristic: title

text: Plasmid

text: pGEM-T Easy vector


text: Max Planck Institute for Chemical Ecology

characteristic: INSDC last update

text: live

text: Generic

text: Heliothis virescens pheromone gland transcriptome analysis; non-normalized cDNA library

text: pheromone gland

text: Heliothis virescens

text: Female

characteristic: sex

text: pheromone gland

text: Hvir PG non-norm

text: Plasmid

text: pDNR-Lib


text: live

text: Generic

text: Heliothis virescens pheromone gland transcriptome analysis; normalized cDNA library

text: pheromone gland

text: Heliothis virescens

text: Female

text: pheromone gland

characteristic: tissue-type

text: Hvir PG norm

text: Plasmid

text: pDNR-Lib

characteristic: vector


text: Institute of Himalayan Bioresource Technology

text: live

characteristic: INSDC status

text: Generic

text: Leaf

text: Sinopodophyllum hexandrum

text: Leaf

text: P450 library of Podophyllum hexandrum royle leaf

text: Plasmid

text: pGEMT-Easy vector (Promega)


text: Faculty of Biology, Plant Physiology

text: live

text: Generic

characteristic: NCBI submission model

text: Seeds of Lepidium sativum (garden cress) were imbibed in 10% MS-medium at 18 degrees and continuous light for 8 and 18 hours, respectively. cDNA synthesised from RNA extracted from the micropylar endosperm cap after 18 hours was used as Tester, against cDNA from RNA, extracted from the micropylar endosperm cap after 8h imbibition as a Driver to enrich for late- germination expressed transcripts. The Suppression Subtraction Hybridisation library was constructed using the PCR Select cDNA Subtraction kit (Clontech, Mountain View, CA). The obtained cDNA fragments were cloned in pTOPO.

text: imbibed seed

characteristic: dev-stage

text: E.coli TOP10

text: seed

text: Lepidium sativum

text: micropylar endosperm

text: late-germination upregulated


text: Genetic Engineering and Biotechnology Institute (GEBI)

text: live

text: Generic

characteristic: NCBI submission package

text: Alpu 01

text: cDNA sequence from Triticum aestivum L. cv. Alpu01 at seedling stage obtained by cDNA AFLP after 150mM NaCI treatment.

text: 28 days-old seedling stage

text: Triticum aestivum

text: leaf

text: 150 mM NaCI treated leaf tissue during 8 hours


text: University of PretoriA

text: live

text: Generic

text: Nabusa (AAA-EA)

text: Root cDNA library post endophyte inoculation of tissue culture banana plantlets. Inoculated with Fusarium oxysporum isolate V5w2. RNA was synthesized into mRNA and Poly A tail of mRNA used in cDNA synthesis. cDNA-AFLPs were performed using LI-COR kit from Biosciences. Polymorphic bands were excised and sequenced using M13-pUC vector primers at Macrogen Corp, Rockville, USA.

text: Root

characteristic: organ

text: Musa acuminata AAA Group

text: Root banana cv. Nabusa (AAA-EA) inoculated with Fusarium oxysporum isolate V5w2


text: University of PretoriA

text: live

text: Generic

text: Nabusa (AAA-EA)

text: Root cDNA library post endophyte inoculation of tissue culture banana plantlets. Inoculated with Fusarium oxysporum isolate Emb2.4o. RNA was synthesized into mRNA and Poly A tail of mRNA used in cDNA synthesis. cDNA-AFLPs were performed using LI-COR kit from Biosciences. Polymorphic bands were excised and sequenced using M13-pUC vector primers at Macrogen Corp, Rockville, USA.

text: Root

text: Musa acuminata AAA Group

characteristic: organism

text: Root banana cv. Nabusa (AAA-EA) inoculated with Fusarium oxysporum isolate Emb2.4o


characteristic: External Id

text: University of PretoriA

text: live

text: Generic

text: Nabusa (AAA-EA)

text: Root cDNA library post endophyte inoculation of tissue culture banana plantlets. Inoculated with Fusarium oxysporum isolates V5w2 and Emb2.4o. RNA was synthesized into mRNA and Poly A tail of mRNA used in cDNA synthesis. cDNA-AFLPs were performed using LI-COR kit from Biosciences. Polymorphic bands were excised and sequenced using M13-pUC vector primers at Macrogen Corp, Rockville, USA.

text: Root

text: Musa acuminata AAA Group

text: Root banana cv. Nabusa (AAA-EA) inoculated with Fusarium oxysporum isolates V5w2 and Emb2.4o


text: University of PretoriA

characteristic: INSDC center name

text: live

text: Generic

text: Nabusa (AAA-EA) and Kayinja (ABB)

text: Root

text: Musa hybrid cultivar

text: Root banana cv. Nabusa (AAA-EA) and Kayinja (ABB) inoculated with Fusarium oxysporum isolates V5w2 and Emb2.4o


text: University of PretoriA

text: live

text: Generic

text: Kayinja (ABB)

text: Root

text: Musa AAB Group

text: Root banana cv. Kayinja (ABB) inoculated with Fusarium oxysporum isolates V5w2 and Emb2.4o


text: University of PretoriA

text: live

text: Generic

text: Nabusa (AAA-EA)

text: Rhizome cDNA library post endophyte inoculation of tissue culture banana plantlets. Inoculated with Fusarium oxysporum isolate V5w2. RNA was synthesized into mRNA and Poly A tail of mRNA used in cDNA synthesis. cDNA-AFLPs were performed using LI-COR kit from Biosciences. Polymorphic bands were excised and sequenced using M13-pUC vector primers at Macrogen Corp, Rockville, USA.

text: Rhizome

text: Musa acuminata AAA Group

text: Rhizome banana cv. Nabusa (AAA-EA) inoculated with Fusarium oxysporum isolate V5w2


text: Cummings School of Veterinary Medicine at Tufts University

text: live

text: Generic

text: most cell types

characteristic: cell type

text: Whole shrimp (cephalotorax and tail) from specific pathogen-free Litopenaeus vannamei challenged with Taura Syndrome Virus in a waterborne assay was used for total RNA isolation. The CLONTECH PCR-SMARTTM cDNA kit was used for library construction.

text: juvenile

text: E. coli (ElectroMAX DH5?-E

characteristic: lab-host

text: whole juvenile shrimp

text: Penaeus vannamei

text: Sfi A (5'end)

text: Sfi B (3'end)

text: most tissue types

text: pDNR-LIB (CLONETECH)


text: Universite Libre de Bruxelles

characteristic: INSDC first public

text: live

text: Generic

text: Six isolates of Suillus luteus, of which the cadmium tolerance was measured in a previous study (Krznaric et al., 2008), were used for a transcriptomic analysis. The three Cd-tolerant isolates (Lc4, Sl24, Lm2) were isolated from a heavy metal contaminated area in Lommel (NE-Belgium), and displayed the following EC50, respectively Lc4 180 uM, Sl24 160 uM, Lm2 160 uM. The three Cd-sensitive ones (P13, Sl34, Sl33) were isolated from a non-polluted site in Paal, (NE-Belgium, Colpaert et al., 2004) and had the following EC50, respectively Sl33 3.6 uM, Sl34 8 uM and P13 13uM. Uniform inocula (0.5 cm2 plugs of fungal mycelium) were prepared from one week old cultures of both isolates, by preincubation of various plugs of mycelium on cellophane-covered agar plates with basic medium for 2 or 3 days. Single inocula were transferred to the test plates containing the nutrient medium with or without added cadmium covered with cellophane and were allowed to grow in the dark for 7 days at 23oC. Five replicates were made for each treatment. In our study, three Cd-sensitive and three Cd-tolerant populations of Suillus luteus isolated from non-polluted or highly polluted regions of Limburg (Belgium) were used for a transcriptomic analysis. We identified differentially expressed genes between the Cd-sensitive and Cd-tolerant populations, having core metabolic functions (e.g. protein and lipid synthesis, modification or catabolism, glycolysis and glyconeogenesis, electron and energy transfer, etc.) as well as putative signaling and regulatory functions. A cDNA AFLP transcript profiling was performed on mycelium of these 6 genotypes, grown on control and Cd-supplemented media (CdSO4 at concentrations of 0 and 9 uM Cd2+) for one week. Transcript derived fragments associated with Cd tolerance, showing different abundance among Cd treatments or between sensitive/tolerant isolates, were cut from AFLP gels, amplified by PCR using Taq DNA polymerase, subsequently cloned in the pTZ57R/T vector (Fermentas GMBH) and sequenced.

text: Suillus luteus

text: XbaI

text: BamHI

text: fungal mycelium

text: Suillus luteus Cd-TDFs [transcript derived fragments (TDFs) associated with Cd tolerance] collection

text: Plasmid

text: pTZ57R/T vector (InsT/AcloneTM PCR Product Cloning Kit; Fermentas)


text: Sun Yat-sen College of Medical Science

text: live

text: Generic

text: country: China:Guangzhou ; lat_lon: 23.08 N 113.15 E ; collected_by: Zhan Ximei

characteristic: description

text: fourth-stage larva

text: Angiostrongylus cantonensis

text: Angiostrongylus cantonensis fourth-stage larva


text: University of Azores

text: live

text: Generic

text: Infective juveniles were incubated with 1% Galleria menonella larvae hemolymph for 6 hours before total RNA was extracted. First-strand cDNA was synthesized using PowerScript reverse transcriptase and amplified by PCR, then was purified and normalized. ds-cDNA were treated with protease K, digested with Sfi restriction endonucleases, ligated to pDNR-LIB (CmR) vector digested with Sfi, transformed into XL10-Gold KanR cells prepared. Pooled transformations were assayed on titre by plating subsequent dilutions on LB-agar plates.

text: Parasitic stage

text: Breton

text: Steinernema carpocapsae

text: Sfi

text: Whole worm

text: Steinernema carpocapsae parasitic stage cDNA library

characteristic: title

text: plasmid

text: pDNR-LIB (CmR)


text: Tokyo Institute of Technology

characteristic: INSDC last update

text: live

text: Generic

text: Homo sapiens

text: Homo sapiens transcripts of unknown function


text: Huazhong Agricultural University

text: live

text: Generic

characteristic: cultivar

text: Gossypium barbadense

text: fiber

text: Gossypium barbadense L. line 3-79 fiber normalized cDNA library


text: U.S. Department of Agriculture

text: live

text: Generic

text: sporulation

text: RGR 91.0166 (EGS 90.0512)

characteristic: isolate

text: Alternaria gaisen

text: mycelium, conidiophores

characteristic: tissue-type

text: A. gaisen cDNA light x dark subtracted


text: National Institute for Plant Genome Research

text: live

characteristic: INSDC status

text: Generic

text: suppression subtractive hybridization analysis for Tomato leaf curl virus tolerance in tomato.

text: 21 days old seedlings

text: Solanum lycopersicum

text: SSH library of ToLCNDV treated tomato


text: Southwest University

text: live

text: Generic

characteristic: NCBI submission model

text: A suppression subtractive library is constructed using B. bassiana wild-type mRNA as tester and the Bbmpk1 disruption mutant mRNA as driver. The targeted genes were screened and cloned into pGEM-T vector to sequence.

text: appressorium formation

text: Escherichia coli DH5alpha

text: Beauveria bassiana

characteristic: strain

text: SSH library enriched for genes regulated by BbMPK1 at the stage of appressorium formation

text: pGEM-T

characteristic: vector


text: Central Institute of Medicinal and Aromatic Plants (CIMAP, CSIR)

text: live

text: Generic

characteristic: NCBI submission package

text: Sampada

text: Poly (A)+ mRNA isolated from the capsule wall of mature Papaver somniferum (cv. Sampada) plant growing in the CIMAP farm at Lucknow under the National Gene Bank for Medicinal and Aromatic Plants, was used for preparing the cDNA library in Lambda Zap Express vector (Stratagene).

text: Mature field-growing plant

characteristic: dev-stage

text: Capsule

characteristic: organ

text: Papaver somniferum

text: Capsule

text: Papaver somniferum capsule wall cDNA library in Lambda Zap Express vector

text: Phagemid

text: pBK-CMV


characteristic: External Id

characteristic: INSDC center name

text: live

text: Generic

text: Sampada

text: Poly (A)+ mRNA isolated from the leaf of mature Papaver somniferum (cv. Sampada) plant growing in the CIMAP farm at Lucknow under the National Gene Bank for Medicinal and Aromatic Plants, was used for preparing the cDNA library in Lambda Zap Express vector (Stratagene).

text: Mature field-growing plant

text: Leaf

text: Papaver somniferum

characteristic: organism

text: Leaf

text: Papaver somniferum leaf cDNA library in Lambda Zap Express vector

text: Phagemid

text: pBK-CMV


text: FNC-CENICAFE, Centro Nacional de Investigaciones de Cafe

characteristic: INSDC first public

text: live

text: Generic

text: Coffea arabica developed flowers

text: developed flowers

text: E.coli

characteristic: lab-host

text: Coffea arabica

text: Flower

text: Plasmid

text: pDNR-LIB (BD Bioscience)


text: live

text: Generic

text: Coffea arabica flower buds

characteristic: description

text: flower buds

text: E.coli

text: Coffea arabica

text: Buds

characteristic: title

text: Plasmid

text: pDNR-LIB (BD Bioscience)


characteristic: INSDC last update

text: live

text: Generic

text: Coffea arabica 28 weeks fruits

text: 28 weeks old fruits

text: E.coli

text: Coffea arabica

text: Fruits

text: Plasmid

text: pDNR-LIB (BD Bioscience)


text: live

text: Generic

text: Coffea arabica var. Caturra young leaves

text: young leaves

text: E.coli

text: Coffea arabica

text: Leaf

text: Plasmid

text: pDNR-LIB (BD Bioscience)


text: live

text: Generic

text: Coffea arabica meristems

text: Meristems

text: E.coli

text: Coffea arabica

text: Meristems

text: Plasmid

text: pDNR-LIB (BD Bioscience)


text: live

text: Generic

text: Coffea arabica 28 weeks old fruits

text: 28 weeks old fruits

text: E.coli

text: Coffea arabica

text: Fruit

text: Plasmid

text: pDNR-LIB (BD Bioscience)


text: live

text: Generic

text: Normalized library constructed at Evrogen, Russia and sequenced at TIGR from RNA extracted from mix.

text: Adult trees

text: E.coli Xl1-Blue

text: Coffea arabica

text: Mixture of leaf, fruit, flower, root and calli

text: Plasmid


text: live

text: Generic

text: Adult trees

text: Coffea arabica

text: Mixture of leaves, flowers, fruits and meristems

text: Plasmid


text: live

text: Generic

text: Coffea arabica 26 weeks old fruits

text: fruits

text: E.coli

text: Coffea arabica

text: Fruits

text: Plasmid

text: pDNR-LIB (BD Bioscience)


text: live

text: Generic

text: Coffea arabica 31 weeks old fruits

text: 31 weeks old fruits

text: E.coli

text: Coffea arabica

text: Fruits

text: Plasmid

text: pDNR-LIB (BD Bioscience)


text: live

text: Generic

text: Coffea arabica 28 weeks old fruits

text: 28 weeks old fruits

text: E.coli

text: Coffea arabica

text: Fruits

text: Plasmid

text: pDNR-LIB (BD Bioscience)


text: live

text: Generic

text: Coffea arabica roots

text: Secondary roots

text: E.coli

text: Coffea arabica

text: Roots

text: Plasmid

text: pDNR-LIB (BD Bioscience)


text: live

text: Generic

text: Coffea arabica parchment

text: Fruit parchment

text: E.coli

text: Coffea arabica

text: parchment

text: Plasmid

text: pDNR-LIB (BD Bioscience)


text: live

text: Generic

text: Induced with H. hampei. 24h. Frutos . Full Length

text: Frutos con 100-150 dias de desarrollo

text: E.coli

text: Coffea arabica

text: Fruits

text: Plasmid

text: pDNR-LIB (BD Bioscience)


text: live

text: Generic

text: After 24h Acibenzolar-S-Methyl induced

text: E.coli

text: Coffea arabica

text: Leaf

text: Plasmid

text: pGEMT-Easy (Promega)


text: live

text: Generic

text: After 192h Acibenzolar-S-Methyl induced

text: 8-month plants

text: E.coli

text: Coffea arabica

text: Leaf

text: Plasmid

text: pGEMT-Easy (Promega)


text: live

text: Generic

text: Induced with H. hampei. 7 days. Sustractiva

text: Frutos con 100-150 dias de desarrollo

text: E.coli

text: Coffea arabica

text: Fruits

text: Plasmid

text: pGEMT-Easy (Promega)


text: live

text: Generic

text: Induced with H. hampei. 24h. Sustractiva

text: Frutos con 100-150 dias de desarrollo

text: E.coli

text: Coffea arabica

text: Fruits

text: Plasmid

text: pGEMT-Easy (Promega)


text: live

text: Generic

text: Induced with H. hampei. 72h. Sustractiva

text: Frutos con 100-150 dias de desarrollo

text: E.coli

text: Coffea arabica

text: Fruits

text: Plasmid

text: pGEMT-Easy (Promega)


text: live

text: Generic

text: Frutos con 100-150 dias de desarrollo

text: E.coli

text: Coffea arabica

text: Leaf

text: Plasmid

text: pGEMT-Easy (Promega)


text: live

text: Generic

text: Hoja Roya 48h after infection

text: E.coli

text: Coffea arabica

text: Leaf

text: Plasmid

text: pGEMT-Easy (Promega)


text: live

text: Generic

text: Timor Hybrid Leaf Rust 48h after infection

text: Deattached young leaves (1st or sd pair)

text: E.coli

text: Coffea arabica

text: Leaf

text: Plasmid

text: pGEMT-Easy (Promega)


text: live

text: Generic

text: Floral buds from adult Coffee plants

text: E.coli

text: Coffea arabica

text: Buds

text: CarCatBudEnri

text: Plasmid


text: live

text: Generic

text: Leaves from juvenil and adult Coffee plants

text: E.coli

text: Coffea arabica

text: Leaf

text: CarCatLeEnri

text: Plasmid


text: live

text: Generic

text: Coffea arabica

text: Leaf

text: Plasmid


text: live

text: Generic

text: Floral buds from adult Coffee plants

text: Coffea arabica

text: Buds

text: Plasmid


text: live

text: Generic

text: Coffea arabica

text: Plasmid


text: Scottish Crop Research Institute

text: live

text: Generic

text: Ben Hope

characteristic: cultivar

text: A directional cDNA library was constructed in pSPORT1 from pooled mRNA using the Superscript Plasmid System (Invitrogen) according to the manufacturers instructions.

text: Pooled week 0 (December), week 2, week 4, week 8 and week 12 bud samples

text: E coli strain DH10B

text: Ribes nigrum

text: SalI

text: NotI

text: Leaf bud

text: Ribes nigrum Ben Hope pooled bud (SCRI_Rn_bud_cDNA)


text: University of Shiga Prefecture

text: live

text: Generic

text: Sequences obtained by degenerate RT-PCR with total RNA and oligo(dT)18, and pDrive vector (Qiagen) to isolate clones.

text: adult

text: Gnathopogon caerulescens

text: mixed

characteristic: sex

text: Lake Biwa

text: brain

characteristic: tissue-type

text: Honmoroko Tissue Library, brain


text: University of Shiga Prefecture

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: adult

text: Gnathopogon caerulescens

text: mixed

text: Lake Biwa

text: gonad

text: Honmoroko Tissue Library, gonad


text: University of Shiga Prefecture

text: live

text: Generic

text: adult

text: Gnathopogon caerulescens

text: mixed

text: Lake Biwa

characteristic: strain

text: gonad and brain

text: Honmoroko Tissue Library, gonad and brain


text: University of Shiga Prefecture

text: live

text: Generic

characteristic: NCBI submission package

text: adult

text: Gnathopogon caerulescens

text: mixed

text: Lake Biwa

text: gonad and kidney

text: Honmoroko Tissue Library, gonad and kidney


text: University of Shiga Prefecture

text: live

text: Generic

text: adult

characteristic: dev-stage

text: Gnathopogon caerulescens

text: mixed

text: Lake Biwa

text: liver

text: Honmoroko Tissue Library, liver


text: University of Shiga Prefecture

text: live

text: Generic

text: adult

text: Gnathopogon caerulescens

text: mixed

text: Lake Biwa

text: whole body

text: Honmoroko Tissue Library, whole body


text: UMR INRA/Universite Henri Poincare 1136

text: live

text: Generic

text: The cDNA libray was constructed from 1 ug of total RNA using the SMART cDNA synthesis kit (Clontech, Palo Alto, CA, USA) according to the manufacturer's instructions. The resulting cDNA was packed into lambda phages using the Gigapack III Gold packaging kit (Stratagene, La Jolla, CA). The pTriplEx2 phagemid clones in Escherichia choli were obtained by using the mass in vivo excision protocol according to the manufacturer's instructions (Clontech).

text: Lb2: 5 to 10 mm differentiating fruitbody, Lb3: 30 to 40 mm mature fruitbody

text: Fruitbody; Lb2: caps and stipes, Lb3: caps

characteristic: organ

text: Laccaria bicolor

text: cDNA libraries of Laccaria bicolor fruitbody

characteristic: vector


characteristic: External Id

text: Gulbarga University

characteristic: INSDC center name

text: live

text: Generic

text: pUC18/HindIII cDNA library was constructed from Radicle tissues of 7 day old Pigeon pea seedlings of WRP-1 genotype challenged with 150mM NaCl stress. The cDNA fragments were cloned into pUC18 vector and subsequently sequenced by Sanger sequencing approach using M13 primers.

text: One week old seedling

text: E. coli (DH5a)

text: Cajanus cajan

text: HindIII

text: Radicle Tissue

text: Salinity stress induced (7 Days) Pigeon pea genotype WRP-1 pUC18/HindIII

text: Plasmid


text: Wuhan University

characteristic: INSDC first public

text: live

text: Generic

text: Adult

text: Lychas mucronatus

characteristic: organism

text: Venom gland

text: Yun Nan scorpion Lychas mucronatus venom gland Library


text: Xiamen University

text: live

text: Generic

text: SSH (suppression subtractive hybridization) cDNA library of the strain A123 and its mutation strain G2-919 which produces over 200 times bioactive compound deacetylmycoepoxydiene(DAM) to its initial strain A123. G2-919 is the 'tester' and A123 is the 'driver' cDNA.

characteristic: description

text: Phomopsis sp. G2-919

text: Phomopsis sp. G2-919 SSH cDNA library


text: National University of Singapore

text: live

text: Generic

text: Five adult female spiders of Nephila antipodiana were collected from the bush in east Singapore. The day before the collection, major part of the spider web was destroyed. Tissues comprising all of the seven types of silk glands were isolated and total RNA was extracted from the silk glands using Trizol reagent (Gibco BRL). Poly-A RNA was purified using an Oligo-dT affinity column (Stratagene). For cDNA library construction, Stratagene's Lambda Uni-Zap XR cloning system was used, following the instructions manual from the manufacturer. In brief, ~5ug of Poly-A RNA was used for cDNA synthesis and two restriction enzymatic digestion sites of XhoI and EcoRI were introduced. cDNA molecules were directionally cloned into the predigested Zap vector and packaged using the package extract. The titer of the primary library reached 9x106 and 5x106 primary clones were amplified for long term storage.

text: adult

text: Trichonephila antipodiana

text: female

text: Singapore local species

text: seven types of silk glands, mixed

text: Spider Nephila antipodiana silk glands cDNA library

characteristic: title


text: Scottish Crop Research Institute

characteristic: INSDC last update

text: live

text: Generic

text: Latham

text: sequenced with M13 forward and reverse primers

text: Rubus idaeus subsp. strigosus

text: Roots

text: Roots from Rubus idaeus subsp. strigosus