text: Lanzhou Veterinary Research Institute

text: live

text: Generic

text: Total RNA were isolated from organs such as salivary glands, Malpighian tubules, ovaries dissected from partially engorged Haemaphysalis qinghaiensis. Subsequently mRNA were purified. A library of oligo(dT)-primed cDNA with added directional EcoR I/Hind III linkers was constructed from the purified mRNA. The constructed cDNA was ligated to the EcoRI/HindIII arms of the screen vector. The recombinant phage DNA was packaged by using PhageMaker packaging extracts, resulting in a primary cDNA library.

text: partially engorged ticks

characteristic: dev-stage

characteristic: organ

text: Haemaphysalis qinghaiensis

text: female

text: cDNA Expression Library of Haemaphysalis qinghaiensis ault Tick

text: Phage


status: PUBLIC

characteristic: External Id

text: NIOO-KNAW

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Soil isolate

tag: attribute

characteristic: isolate

text: Pseudomonas sp. A21

characteristic: organism

characteristic: strain

text: Pseudomonas sp. A21 RAP-PCR

characteristic: title


text: NIOO-KNAW

text: live

text: Generic

text: Soil isolate

text: Pedobacter sp. V48

text: Pedobacter sp. V48 RAP-PCR


text: University of Missouri, National Center for Soybean Biotechnology, Division of Plant Sciences and Biochemistry

text: live

text: Generic

text: Early gallatin

characteristic: cultivar

text: ESTs were sequenced from a normalized, subtracted library. mRNA was extracted from bean leaves (cv. early gallatin) infected by the bean rust pathogen (U. appendiculatus). This mRNA was subtracted against normal leaf (uninfected) RNA and from RNA from germinated U. appendiculatus spores. mRNA were extracted from plants infected with either a virulent fungal strain, race41, or an avirulent strain, race49. The RNA was extracted from leaves 0-6-12-15-24-48-72-96-120 hours after inoculation. The resulting cDNA was pooled into early (0-24 h) and late (48-120 h) fractions and tagged during cDNA preparation so that they could be placed into the early and late fractions after sequencing. The cDNA was cloned into pGEMT and sequenced with M13 primer.

characteristic: description

text: Leaf

text: Phaseolus vulgaris

text: Phaseolus vulgaris cv Early gallatin

text: pGEMT

characteristic: vector


text: University of Illinois

text: live

text: Generic

text: The library was constructed and normalized as described by Bonaldo, M.F., Lennon, G. and Soares, M.B. (1996), Genome Research 6(9): 791-806. Previously sequenced SB02 clones were subtracted from the library. An identiying tag was added at the 3'during cDNA synthesis: insertAAAAAAAAAAAAAAAAAATGCGA.

text: Late embryo, post-hatch days 1, 10, 20, 45, and adult (pooled)

characteristic: lab-host

text: Brain

text: Taeniopygia guttata

text: EcoRI(5' side of insert)

text: NotI (3' side of insert)

text: Brain

characteristic: tissue-type

text: Normalized subtracted Keck-Tagu Library SB05

text: Plasmid

text: pBS II SK(+)


text: University of Illinois

text: live

text: Generic

characteristic: dev-stage

text: Brain

characteristic: organ

text: Taeniopygia guttata

text: EcoRI(5' side of insert)

text: NotI (3' side of insert)

text: Brain

text: Normalized subtracted Keck-Tagu Library SB06

text: Plasmid

text: pBS II SK(+)


characteristic: External Id

text: University of Victoria

characteristic: INSDC center name

text: live

text: Generic

text: Tissue contributors: Vanya Ewart (NRC Institute for Marine Biociences, Halifax, N.S.)

text: Osmerus mordax

text: brain

text: pBlueScriptIISK+


text: University of Victoria

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Tissue contributors: Craig Primmer, Department of Biology, University of Turku, Finland

text: Thymallus thymallus

text: brain, pituitary, kidney, spleen


text: University of Victoria

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Tissue contributors: Carlo Biagi, Mitch Uh and Robert Devlin (DFO, Vancouver, B.C.), Simon Jones (PBS, Nanaimo, B.C.), Seaspring Hatchery (Crofton, B.C.), Rachel Roper (University of Victoria)

text: Salmo salar

text: liver, head kidney, spleen

text: pBlueScriptIISK+


text: University of Victoria

text: live

text: Generic

characteristic: NCBI submission package

text: Salmo salar

text: liver, head kidney, spleen


text: University of Victoria

text: live

text: Generic

text: Osmerus mordax

characteristic: organism

text: kidney

text: pBlueScriptIISK+


text: University of Victoria

text: live

text: Generic

text: Osmerus mordax

text: spleen

text: pBlueScriptIISK+


text: Pioneer Hi-Bred International, Inc., A Dupont Company

text: live

text: Generic

text: Colletotrichum graminicola

characteristic: strain

text: Colletotrichum graminicola cDNA sequence


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT)-primed cDNA and cloned into vector pTriplEx2 (Clontech Laboratories, Inc., Mountain View, CA) using the SMART system (BD Biosciences, San Jose, CA). To generate the first strand, polyA RNA was primed with an oligo(dT) primer (5'-ATTCTAGAGGCCGAGGCGGCCGACATG(T)30 VN-3', where V = A, G, or C). The second strand was synthesized by PCR using primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' followed by digestion of the SfiI sites. Library constructed in the laboratory of J. Stillman (San Francisco State University, San Francisco, CA) and sequencing performed by the DOE Joint Genome Institute (Walnut Creek, CA). Additional information can be found in the Porcelain Crab Array Database; http://array.sfsu.edu.

text: adult

text: Petrolisthes cinctipes

text: SfiIA

text: SfiIB

text: mixed

characteristic: sex

text: pooled heart, nerve, whole crab

text: CAYC Petrolisthes cinctipes heart, nerve, whole crab

characteristic: title


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT)-primed cDNA and cloned into vector pTB (gift from A. Gracey) using the SMART system (BD Biosciences, San Jose, CA). Library constructed in the laboratory of J. Stillman (San Francisco State University, San Francisco, CA) and sequencing performed by the DOE Joint Genome Institute (Walnut Creek, CA). Additional information can be found in the Porcelain Crab Array Database; http://array.sfsu.edu.

characteristic: description

text: adult

text: Petrolisthes cinctipes

text: SfiIA

text: SfiIB

text: mixed

text: pooled heart, whole crab, hepatopancreas, claw, gill

text: CAYF Petrolisthes cinctipes heart, whole crab, hepatopancreas, claw, gill

text: pTB


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: broad array of treatments including thermal stress, salinity stress, heavy metal stress, selenium stress, insecticide stress, nutritional stress, wild-caught specimens, laboratory acclimated specimens. The library was made from oligo(dT)-primed cDNA and cloned into vector pDNR-LIB (Clontech Laboratories, Inc., Mountain View, CA) using the SMART system (BD Biosciences, San Jose, CA). To generate the first strand, polyA RNA was primed with an oligo(dT) primer (5'-ATTCTAGAGGCCGAGGCGGCCGACATG(T)30 VN-3', where V = A, G, or C). The second strand was synthesized by PCR using primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' followed by digestion of the SfiI sites. cDNA was size selected then ligated into pDNR-LIB. Library is normalized. Library was created and sequencing done by the DOE Joint Genome Institute (Walnut Creek, CA). Additional information can be found in the Porcelain Crab Array Database; http://array.sfsu.edu.

text: pool of adult, larval, freshly molted

text: Petrolisthes cinctipes

text: SfiIA

text: SfiIB

text: mixed

text: pooled heart, gills, whole crab

text: CCAG Petrolisthes cinctipes heart, gills, whole crab (N)

text: pDNR-LIB

characteristic: vector


text: live

text: Generic

text: Ctenopharyngodon idella

text: female

text: intestine

text: Grass carp intestinal cDNA library


text: Beijing Forestry University

text: live

text: Generic

text: Two groups of mRNA was extracted. One is from the anual leaf of Ophiopogon japonicus which was treated with 0.5g/L entrel solution for 6 hours. The other group is from the anual leaf of Ophiopogon japonicus which was treated with distilled water for 6 hours. We got cDNA from mRNA by reverse transcription PCR. Then we got cDNA library by SSH method.

text: mature

characteristic: lab-host

text: Ophiopogon japonicus

text: annual leaf

text: Ethrel induced Ophiopogon japonicus (L.f.) KerGawl cDNA Library

text: pGEM-T Easy


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: aerobic dextrose. The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected >2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed at DOE Joint Genome Institute (Walnut Creek, CA).

text: Scheffersomyces stipitis

text: SalI

text: NotI

text: CAFG Pichia stipitis aerobic dextrose (AD) H


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: aerobic dextrose. The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 1.2-2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed at DOE Joint Genome Institute (Walnut Creek, CA).

text: Scheffersomyces stipitis

text: SalI

text: NotI

text: CAFH Pichia stipitis aerobic dextrose (AD) M


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: aerobic dextrose. The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 0.6-1.2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed at DOE Joint Genome Institute (Walnut Creek, CA).

text: Scheffersomyces stipitis

text: SalI

text: NotI

text: CAFI Pichia stipitis aerobic dextrose (AD) L


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: aerobic xylose. The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected >2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed at DOE Joint Genome Institute (Walnut Creek, CA).

text: Scheffersomyces stipitis

text: SalI

text: NotI

text: CAFN Pichia stipitis aerobic xylose H


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: aerobic xylose. The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 1.2-2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed at DOE Joint Genome Institute (Walnut Creek, CA).

text: Scheffersomyces stipitis

text: SalI

text: NotI

text: CAFO Pichia stipitis aerobic xylose M


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: aerobic xylose. The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 0.6-1.2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed at DOE Joint Genome Institute (Walnut Creek, CA).

text: Scheffersomyces stipitis

text: SalI

text: NotI

text: CAFP Pichia stipitis aerobic xylose L


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: oxygen limited dextrose. The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected >2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed at DOE Joint Genome Institute (Walnut Creek, CA).

text: Scheffersomyces stipitis

text: SalI

text: NotI

text: CAFS Pichia stipitis oxygen limited dextrose H


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: oxygen limited dextrose. The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 1.2-2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed at DOE Joint Genome Institute (Walnut Creek, CA).

text: Scheffersomyces stipitis

text: SalI

text: NotI

text: CAFT Pichia stipitis oxygen limited dextrose M


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: oxygen limited dextrose. The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 0.6-1.2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed at DOE Joint Genome Institute (Walnut Creek, CA).

text: Scheffersomyces stipitis

text: SalI

text: NotI

text: CAFU Pichia stipitis oxygen limited dextrose L


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: oxygen limited xylose. The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected >2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed at DOE Joint Genome Institute (Walnut Creek, CA).

text: Scheffersomyces stipitis

text: SalI

text: NotI

text: CAFW Pichia stipitis oxygen limited xylose H


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: oxygen limited xylose. The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 1.2-2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed at DOE Joint Genome Institute (Walnut Creek, CA).

text: Scheffersomyces stipitis

text: SalI

text: NotI

text: CAFX Pichia stipitis oxygen limited xylose M


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: oxygen limited xylose. The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 0.6-1.2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed at DOE Joint Genome Institute (Walnut Creek, CA).

text: Scheffersomyces stipitis

text: SalI

text: NotI

text: CAFY Pichia stipitis oxygen limited xylose L


text: College of Animal Science and Technology, Hunan Agricultural University

text: live

text: Generic

text: ESTs related to Piglet's Escherichia coli F4 Receptor by using differential display RT-PCR

text: small intestine

text: Sus scrofa

text: Yorkshire (Large white)

text: small intestine

characteristic: tissue-type

text: Piglet, disease resistance, small intestine


text: Anhui Agricultural Univerisity

text: live

text: Generic

characteristic: cultivar

text: Camellia sinensis

text: Sfi I A

text: Sfi I B

text: tender root

text: Tender roots cDNA library of tea plant

text: pBluescript II SK


text: National Institute of Allergy and Infectious Diseases

text: live

text: Generic

text: Secretory

characteristic: cell type

text: PCR-based cDNA library made with SMART cDNA library construction kit from Clontech

text: Salivary gland

characteristic: organ

text: Ornithodoros coriaceus

text: Salivary gland

text: Ornithodoros coriaceus salivary gland OCSG-1

text: Phage

text: Lambda TriplEx2


characteristic: External Id

text: AgResearch Limited

characteristic: INSDC center name

text: live

text: Generic

text: Library sequenced by University of Florida ICBR Core Center (Gainesville, FL, USA). Library made by Drion Boucias.

text: L3 larvae

characteristic: dev-stage

text: Costelytra zealandica

text: Midgut

text: (CoZea) Costelytra zealandica Healthy Gut

text: pDNR-LIB


text: DOE Joint Genome Institute

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Library constructed in the laboratory of N. King (University of California, Berkeley) and sequencing performed by the DOE Joint Genome Institute (Walnut Creek, CA).

text: rapidly growing cells

text: Monosiga brevicollis

text: XYM Monosiga brevicollis rapidly growing cells


text: Institut Universitaire Europeen de la Mer, Universite de Bretagne Occidentale

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Post-larvae to copper exposure

text: post-larvae

text: Argopecten purpuratus

characteristic: organism

text: subtracted libraries from Argopecten purpuratus post-larva to copper exposure


text: Huazhong Agricultural University

text: live

text: Generic

text: early flowering mutant

text: The subtracted library was obtained by the juvenile wild type mRNAs as tester and cDNA from juvenile mutant mRNAs as driver. In January of 2005, the seeds of precocious trifoliate orange and wild type trifoliate orange were planted in 20 cm pots containing potting mix of a commercial medium and perlite at a ratio of 3:1. These juvenile trees were watered regularly with nutrient solution. Shoot meristem of the juvenile mutant and wild type trees were collected in March, June, September, and December, respectively. To prepare a representative sample of total RNA from the juvenile mutant and wild type in SSH process, different developmental stages of plant organs from roughly equal numbers of the juvenile mutant and wild type material were pooled, respectively. SSH library was performed using PCR Select cDNA Subtraction kit (Clontech, USA) according to the manufacturer's instructions. The final PCR products were purified using QIAquick PCR purification kit (Qiagen). The fragments obtained were cloned into the pGEM-T vector (Promega, USA) and used for the transformation of Escherichia coli DH5a. Individual clones from the library were randomly picked and stored in 384-well plates. A total of 3840 clones were collected in the forward subtracted and the reverse subtracted library. The inserts were amplified with the solution of 1ul single clone bacterial culture as PCR template, 0.5ul primer T7 (10umol/ul), 0.5ul primer SP6 (10umol/ul), 0.25 ul Taq DNA polymerase (Shanghai Sangon, China), 2.5 ul 10 x DNA polymerase buffer, 0.5 ul dNTPs (10mmo/ul) and 1.5 ul MgCl2 (25 mmol/ul), and added ddH2o to 25 ul. The PCR program is: 94 oC for 5 min; 94 oC for 30 s, 58 oC for 35s, 72 oC for 1.5 min, 30 cycles followed by a 5 min final extension at 72 oC.

text: juvenile

text: Escherichia coli DH5alpha

text: Citrus trifoliata

text: Shoot meristem

text: juvenile early flowering trifoliate orange mutant subtracted cDNA library

characteristic: title

text: PGEM-T


text: Huazhong Agricultural University

text: live

text: Generic

text: Wild type

text: The subtracted library was obtained by using cDNA from the juvenile wild type mRNAs as driver and cDNA from juvenile mutant mRNAs as tester. In January of 2005, the seeds of precocious trifoliate orange and wild type trifoliate orange were planted in 20 cm pots containing potting mix of a commercial medium and perlite at a ratio of 3:1. These juvenile trees were watered regularly with nutrient solution. Shoot meristem of the juvenile mutant and wild type trees were collected in March, June, September, and December, respectively. To prepare a representative sample of total RNA from the juvenile mutant and wild type in SSH process, different developmental stages of plant organs from roughly equal numbers of the juvenile mutant and wild type material were pooled, respectively. SSH library was performed using PCR Select cDNA Subtraction kit (Clontech, USA) according to the manufacturer's instructions. The final PCR products were purified using QIAquick PCR purification kit (Qiagen). The fragments obtained were cloned into the pGEM-T vector (Promega, USA) and used for the transformation of Escherichia coli DH5a. Individual clones from the library were randomly picked and stored in 384-well plates. A total of 3840 clones were collected in the forward subtracted and the reverse subtracted library. The inserts were amplified with the solution of 1ul single clone bacterial culture as PCR template, 0.5ul primer T7 (10umol/ul), 0.5ul primer SP6 (10umol/ul), 0.25 ul Taq DNA polymerase (Shanghai Sangon, China), 2.5 ul 10 x DNA polymerase buffer, 0.5 ul dNTPs (10mmo/ul) and 1.5 ul MgCl2 (25 mmol/ul), and added ddH2o to 25 ul. The PCR program is: 94 oC for 5 min; 94 oC for 30 s, 58 oC for 35s, 72 oC for 1.5 min, 30 cycles followed by a 5 min final extension at 72 oC.

characteristic: description

text: juvenile

text: Escherichia coli DH5alpha

text: Citrus trifoliata

text: Shoot meristem

text: juvenile wild type trifoliate orange mutant subtracted cDNA library

text: PGEM-T


text: Washington University School of Medicine

text: live

text: Generic

text: The library was constructed by Irina Ronko and Dr. Makedonka Mitreva at Washington University, St. Louis. The embryos were provided by Dr. Richard E. Davis (Richard.Davis@UCHSC.edu) of University of Colorado School of Medicine.

text: 24hr Embryo

text: GC10 cells

text: Ascaris suum

text: Ecor I

text: Ecor I

text: plasmid

characteristic: vector


text: Huazhong Agricultural University

text: live

text: Generic

text: Early flowering trifoliate orange mutant (precocious trifoliate orange)

text: The subtracted library was obtained by using cDNAs from the adult mRNAs as driver and cDNAs from juvenile mRNAs as tester. Adult and juvenile precocious trifoliate orange samples were collected in the experiment fields of National Citrus Breeding Center in Huazhong Agriculture University. Several plant organs (lateral buds, terminal buds, and shoot meristems) at different developmental stages were sampled and immediately frozen in liquid nitrogen, and then stored at 80oC until use. Adult precocious trifoliate orange have three main flushes of growth. The main one occurs in late winter or early spring (spring flush), which were sprouted and developed from a brunch of a tree in March. In 2005, young buds in the axils of leaves (lateral buds) and terminal buds of spring flushes were collected at equivalent branch positions in May, July, September, November (dormancy period), respectively. In 2006, swelling buds of last year spring flushes were collected in January and March. The juvenile trees are from potted seedlings and grown under field conditions. In January of 2005, the seeds of precocious trifoliate orange were planted in 20 cm pots containing potting mix of a commercial medium and perlite at a ratio of 3:1, the juvenile trees were watered regularly with nutrient solution. Shoot meristems of the juvenile trees were collected in March, June, September, and December, respectively. To prepare a representative sample of total RNA from the adult and juvenile precocious trifoliate orange in SSH process, different developmental stages of plant organs from roughly equal numbers of the adult and juvenile precocious trifoliate oranges were pooled, respectively. SSH library was performed using PCR Select cDNA Subtraction kit (Clontech, USA) according to the manufacturer's instructions. The final PCR products were purified using QIAquick PCR purification kit (Qiagen). The fragments obtained were cloned into the pGEM-T vector (Promega, USA) and used for the transformation of Escherichia coli DH5a. Individual clones from the library were randomly picked and stored in 384-well plates. A total of 3840 clones were collected in the forward subtracted and the reverse subtracted library. The inserts were amplified with the solution of 1ul single clone bacterial culture as PCR template, 0.5ul primer 1 (10?mol/ul), 0.5ul primer 2R (10umol/ul), 0.25 ul Taq DNA polymerase (Shanghai Sangon, China), 2.5 ul 10 x DNA polymerase buffer, 0.5 ul dNTPs (10mmo/ul) and 1.5 ul MgCl2 (25 mmol/ul), and added ddH2o to 25 ul. The PCR program is: 94 oC for 5 min; 94 oC for 30 s, 58 oC for 35s, 72 oC for 1.5 min, 30 cycles followed by a 5 min final extension at 72 oC.

text: Juvenile

text: Escherichia coli DH5alpha

characteristic: lab-host

text: Citrus trifoliata

text: Shoot meristem

text: Juvenile adult early flowering trifoliate orange mutant subtracted cDNA library

text: PGEM-T


text: Huazhong Agricultural University

text: live

text: Generic

text: The subtracted library was obtained by using cDNAs from the juvenile mRNAs as driver and cDNAs from adult mRNAs as tester. Adult and juvenile precocious trifoliate orange samples were collected in the experiment fields of National Citrus Breeding Center in Huazhong Agriculture University. Several plant organs (lateral buds, terminal buds, and shoot meristems) at different developmental stages were sampled and immediately frozen in liquid nitrogen, and then stored at 80oC until use. Adult precocious trifoliate orange have three main flushes of growth. The main one occurs in late winter or early spring (spring flush), which were sprouted and developed from a brunch of a tree in March. In 2005, young buds in the axils of leaves (lateral buds) and terminal buds of spring flushes were collected at equivalent branch positions in May, July, September, November (dormancy period), respectively. In 2006, swelling buds of last year spring flushes were collected in January and March. The juvenile trees are from potted seedlings and grown under field conditions. In January of 2005, the seeds of precocious trifoliate orange were planted in 20 cm pots containing potting mix of a commercial medium and perlite at a ratio of 3:1, the juvenile trees were watered regularly with nutrient solution. Shoot meristems of the juvenile trees were collected in March, June, September, and December, respectively. To prepare a representative sample of total RNA from the adult and juvenile precocious trifoliate orange in SSH process, different developmental stages of plant organs from roughly equal numbers of the adult and juvenile precocious trifoliate oranges were pooled, respectively. SSH library was performed using PCR Select cDNA Subtraction kit (Clontech, USA) according to the manufacturer's instructions. The final PCR products were purified using QIAquick PCR purification kit (Qiagen). The fragments obtained were cloned into the pGEM-T vector (Promega, USA) and used for the transformation of Escherichia coli DH5a. Individual clones from the library were randomly picked and stored in 384-well plates. A total of 3840 clones were collected in the forward subtracted and the reverse subtracted library. The inserts were amplified with the solution of 1ul single clone bacterial culture as PCR template, 0.5ul primer 1 (10umol/ul), 0.5ul primer 2R (10umol/ul), 0.25 ul Taq DNA polymerase (Shanghai Sangon, China), 2.5 ul 10 x DNA polymerase buffer, 0.5 ul dNTPs (10mmo/ul) and 1.5 ul MgCl2 (25 mmol/ul), and added ddH2o to 25 ul. The PCR program is: 94 oC for 5 min; 94 oC for 30 s, 58 oC for 35s, 72 oC for 1.5 min, 30 cycles followed by a 5 min final extension at 72 oC.

text: Adult

text: Escherichia coli DH5alpha

text: Citrus trifoliata

characteristic: tissue-type

text: Adult juvenile early flowering trifoliate orange mutant subtracted cDNA library

text: PGEM-T


text: Institution of Animal Science, CAAS

text: live

text: Generic

text: 'zhongmu' NO.1 cultivar

characteristic: cultivar

text: Ten days after seeding in silicon dioxide and then under salt stress for 6h

text: Medicago sativa

text: whole plant

text: Alfalfa SSH cDNA library induced by salt stress

text: Plasmid


text: Chongqing University

text: live

text: Generic

text: Tanacetum cinerariifolium

text: flower

text: Chrysanthemum Cinerariifolium Flower cDNA library

text: pDNR-LIB


text: Jilin Agricultural University

text: live

text: Generic

text: Panax ginseng

text: callus

text: Ginsenosides synthesis gene regulated by elicitor


text: Middle East Technical University

text: live

text: Generic

text: DDRT-PCR band

text: Triticum aestivum

text: Triticum aestivum AvocetYr1 inoculated with Yellow Rust (race:PST45)


text: Middle East Technical University

text: live

text: Generic

text: DDRT-PCR band

text: Triticum aestivum

text: Triticum aestivum AvocetYr10 inoculated with Yellow Rust (race:PST17)


text: Key Laboratory of Horticulture Plant Germanplasm and Genetic Improvement, Northwest A & F University

text: live

text: Generic

text: plants with little buds were treated by centigrade 7 for 9 days, then grown under centigrade 24. The ten samples of floral buds less than 1.0mm picked from the fifth day to the fourteenth day and these were used for the RNA extraction. Differentially-expressed fragments were seeked for by cDNA Amplified Fragment Length Polymorphism. Differential bands were recovered, cloned and sequenced. This work was supported by the National 863 Plan (2006BAD01A7-1-03) and 13115 project of Shannxi Province (2007ZDKG-05). Sequences have been trimmed to remove vector and primer sequences.

text: floral buds less than 1 mm size

text: floral buds

text: Brassica rapa subsp. pekinensis

text: Low temperature-treated Brassica campestris L. ssp. Pekinensis


text: The Breed & Biotechnology Key Laboratory of Sheep

text: live

text: Generic

text: mature proglottid of adult

text: Moniezia expansa

text: Shihezi

characteristic: strain

text: Moniezia expansa Shihezi mature proglottids


text: Middle East Technical University

text: live

text: Generic

text: Gypsophila perfoliata

text: Gypsophila perfoliata DDRT-PCR clone


text: Teagasc

text: live

text: Generic

text: SSH library generated using pooled tissue from days 3-6 of secondary induction and using earlier and later flowering plants as tester and driver respectively.

text: Lolium perenne

text: Shoot apical meristem

text: pCR 2.1


text: Teagasc

text: live

text: Generic

text: SSH library generated using pooled tissue from days 3-6 of secondary induction and using later and earlier flowering plants as tester and driver respectively.

text: Lolium perenne

text: Shoot apical meristem

text: pCR 2.1


text: Teagasc

text: live

text: Generic

text: SSH library generated after vernalization using later and earlier flowering plants as tester and driver respectively.

text: Lolium perenne

text: Shoot apical meristem

text: pCR 2.1


text: Washington University School of Medicine

text: live

text: Generic

text: The library was constructed by Irina Ronko and Dr. Makedonka Mitreva at Washington University, St. Louis. The embryos were provided by Dr. Richard E. Davis (Richard.Davis@UCHSC.edu) of University of Colorado School of Medicine.

text: 64.5hr Embryo

text: DH10B cells

text: Ascaris suum

text: BsrgI

text: BsrgI

text: plasmid


text: live

text: Generic

text: The library was constructed by Irina Ronko and Dr. Makedonka Mitreva at Washington University, St. Louis. Embryos were provided by Dr. Richard E. Davis (Richard.Davis@UCHSC.edu) of University of Colorado School of Medicine.

text: 96hr Embryo

text: DH10B cells

text: Ascaris suum

text: BsrgI

text: BsrgI

text: plasmid


text: live

text: Generic

text: the EST library was constructed using Invitrogen's CloneMiner cDNA Library construction kit by Irina Ronko and Dr. Makedonka Mitreva at Washington University, St. Louis. The embryos were provided by Dr. Richard E. Davis (Richard.Davis@UCHSC.edu) of University of Colorado School of Medicine.

text: DH10B cells

text: Ascaris suum

text: BsrgI

text: BsrgI

text: 1 cell embryo(24hr non-coated)

text: plasmid


text: live

text: Generic

text: The EST library was constructed using Invitrogen's CloneMiner cDNA Library construction kit by Irina Ronko and Dr. Makedonka Mitreva at Washington University, St. Louis. The intestine tissue was provided by Dr. Alan Scott (ascott@jhsph.edu) of John Hopkins University in Baltimore, MD.

text: DH10B cells

text: Ascaris suum

text: BsrgI

text: BsrgI

text: Female Intestine

text: plasmid


text: live

text: Generic

text: The EST library was constructed using Invitrogen's CloneMiner cDNA Library construction kit by Irina Ronko and Dr. Makedonka Mitreva at Washington University, St. Louis. The A. suum uterus was provided by Dr. Alan Scott (ascott@jhsph.edu) of John Hopkins University in Baltimore, MD.

text: DH10B cells

text: Ascaris suum

text: BsrgI

text: BsrgI

text: Uterus

text: plasmid


text: live

text: Generic

text: Mixed stages of whole nematode

text: DH10B cells

text: Ditylenchus africanus

text: SfiI

text: SfiI

text: mixed

characteristic: sex

text: plasmid

text: pDNR-LIB


characteristic: External Id

text: live

text: Generic

text: DH10B cells

text: Pristionchus pacificus

text: BsrgI

text: BsrgI

text: plasmid

text: pDONR


text: College of Life Science, Xinyang Normal University

characteristic: INSDC center name

text: live

text: Generic

text: Camellia sinensis

text: young leaves

text: Subtracted cDNA library of tea plants exposed to low temperature


text: Institute of Himalayan Bioresource Technology

text: live

text: Generic

text: Teenali

text: Apical Bud

characteristic: organ

text: Camellia sinensis

text: Apical Bud

text: Camellia sinensis winter dormancy suppression subtractive hybridization library

text: Plasmid

text: pGEMT-Easy vector


text: Centre for DNA Fingerprinting and Diagnostics

characteristic: INSDC first public

text: live

text: Generic

text: Directional cDNA library was constructed by cloning cDNA fragments into pBluescript II SK(+) vector and sequenced from 5' end

text: 96 hours embryo

characteristic: dev-stage

text: Antheraea assamensis

text: mixed

text: Embryo

text: Antheraea assama 96 hours embryo cDNA library


text: National Diagnostics Centre

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Suppression subtractive hybridization library

text: february smolt vs april smolt

text: Salmo salar

text: Brain

text: Salmo salar Brain SSH forward february smolt vs april smolt


text: National Diagnostics Centre

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: february smolt vs april smolt

text: Salmo salar

text: Brain

text: Salmo salar Brain SSH Reverse february smolt vs april smolt


text: National Diagnostics Centre

text: live

text: Generic

text: Pre blastula vs post blastula

text: Salmo salar

characteristic: organism

text: Embryos

text: Salmo salar Embryos SSH reverse Pre blastula vs post blastula

characteristic: title


text: National Diagnostics Centre

text: live

text: Generic

characteristic: description

text: smolt april vs smolt may

text: Salmo salar

text: Head Kidney

text: Salmo salar Head Kidney SSH Forward smolt april vs smolt may


text: National Diagnostics Centre

text: live

text: Generic

text: Salmo salar

text: Ovary

text: Salmo salar Ovary SSH forward


text: National Diagnostics Centre

text: live

text: Generic

text: mature female vs non mature female

text: Salmo salar

text: Ovary

text: Salmo salar Ovary SSH reverse mature female vs non mature female


text: National Diagnostics Centre

text: live

text: Generic

text: smolt april vs smolt may

text: Salmo salar

text: Pituitary

text: Salmo salar Pituitary SSH Reverse smolt april vs smolt may


text: National Diagnostics Centre

text: live

text: Generic

text: february smolt vs april smolt

text: Salmo salar

text: Pituitary

text: Salmo salar Pituitary SSH forward february smolt vs april smolt


text: National Diagnostics Centre

text: live

text: Generic

text: february smolt vs april smolt

text: Salmo salar

text: Pituitary

text: Salmo salar Pituitary SSH Reverse february smolt vs april smolt


text: National Diagnostics Centre

text: live

text: Generic

text: grilse female vs mature female

text: Salmo salar

text: Pituitary

text: Salmo salar Pituitary SSH Forward grilse female vs mature female


text: National Diagnostics Centre

text: live

text: Generic

text: grilse female vs mature female

text: Salmo salar

text: Pituitary

text: Salmo salar Pituitary SSH reverse grilse female vs mature female


text: National Diagnostics Centre

text: live

text: Generic

text: parr female vs Grilse female

text: Salmo salar

text: Pituitary

text: Salmo salar Pituitary SSH Reverse parr female vs Grilse female


text: National Diagnostics Centre

text: live

text: Generic

text: parr vs precocious parr

text: Salmo salar

text: Pituitary

text: Salmo salar Pituitary SSH forward parr vs precocious parr


text: National Diagnostics Centre

text: live

text: Generic

text: parr vs precocious parr

text: Salmo salar

text: Pituitary

text: Salmo salar Pituitary SSH reverse parr vs precocious parr


text: National Diagnostics Centre

text: live

text: Generic

text: parr vs precocious parr

text: Salmo salar

text: Testis

text: Salmo salar Testis SSH forward parr vs precocious parr


text: National Diagnostics Centre

text: live

text: Generic

text: parr vs precocious parr

text: Salmo salar

text: Testis

text: Salmo salar Testis SSH reverse parr vs precocious parr


text: University of Calgary

text: live

text: Generic

characteristic: cell-line

text: Papaver somniferum

text: EcoRI

text: XhoI

text: Cell culture

text: Opium poppy elicitor-treated cell culture cDNA library

text: Phage

text: Lambda ZAP; pBluescript

characteristic: vector


text: Institute of Himalayan Bioresource Technology

text: live

text: Generic

text: Leaf

text: Caragana jubata

text: Leaf

characteristic: tissue-type

text: Caragana jubata low-temperature inducible suppression subtractive hybridization (SSH) library

text: plasmid

text: pGEM-T Easy (Promega)


text: College of Horticulture, Northwest A&F University

text: live

text: Generic

characteristic: cultivar

text: 1-7 days after inoculation with Uncinula necator

text: E. coli TOP 10

characteristic: lab-host

text: leaves

text: Vitis pseudoreticulata

text: Rsa I

text: SSH cDNA library of grape vine (Chinese Wild Vitis pseudoreticulata, Baihe-35-1)

text: plasmid

text: pGEM-T Easy


characteristic: External Id

text: Kazusa DNA Research Institute

text: live

text: Generic

text: Lotus japonicus

text: ecotype Gifu B-129

characteristic: strain

text: mature nodule

text: Lotus japonicus mature nodule


text: Kazusa DNA Research Institute

characteristic: INSDC center name

text: live

text: Generic

text: pod (20-30 mm in length)

text: Lotus japonicus

text: EcoRI

text: XhoI

text: ecotype Miyakojima MG-20

text: pod

text: Lotus japonicus pod (20-30 mm in length)

text: pBluescriptII SK-


text: Kazusa DNA Research Institute

characteristic: INSDC first public

text: live

text: Generic

text: root of hypernodulating mutant har1-3, rich in nodule primordia

text: Lotus japonicus

text: ecotype Gifu B-129

text: nodulating root

text: Lotus japonicus nodulating root har1-3


text: AgResearch Ltd.

text: live

text: Generic

text: Bovine mammary (teat) cDNA library derived from tissue supplied by Steve Davis

text: Bos taurus

text: teat canal epithelium

text: BUTA

text: plasmid

text: pBK-CMV


text: Murdoch University

text: live

text: Generic

text: Meloidogyne javanica-induced giant cell

characteristic: cell type

text: Gross Lisse

text: Developing giant cell

characteristic: dev-stage

text: Solanum lycopersicum

text: root

text: Lycopersicon esculentum Meloidogyne javanica-induced early giant cell cDNA transcripts


text: Prince of Songkla University

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: cDNA library constructed by STRATAGENE ZAP Express(r) cDNA Synthsis Kit.

text: Latex

characteristic: organ

text: Hevea brasiliensis

text: EcoR I

text: Xho I

text: Latex cDNA library

text: pBK-CMV


text: USDA, Agricultural Research Service

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Canadian Harmony on Tennessee Natural rootstock

text: Total RNA was prepared from peach bark and used as a template for cDNA synthesis using the SuperSMART system (Clontech, Palo Alto, CA). Amplification of cDNAs and suppression subtractive hybridization (SSH) followed the manufacturer's protocol for SSH (Clontech). PCR-amplified products resulting from two rounds of hybridization were cloned non-directionally into pCR2.1 (Invitrogen, Carlsbad, CA). Colony PCR using M13 forward and reverse primers was used to identify recombinant plasmids containing inserts >200 bp. Recombinant plasmid DNA was sequenced in both directions using Nested PCR primers 1 and 2R (Clontech) or M13 forward and reverse primers. The sequences obtained from the B Forward library represent sequences upregulated during long day photoperiods (16 h light/8 h dark) at 25oC.

text: current year's growth

text: E. coli DH5alpha

text: branch

text: Prunus persica

characteristic: organism

text: monoecious

characteristic: sex

text: bark (mostly cambium)

text: Subtraction suppressive hybridization library B Forward

text: plasmid


text: USDA, Agricultural Research Service

text: live

text: Generic

text: Total RNA was prepared from peach bark and used as a template for cDNA synthesis using the SuperSMART system (Clontech, Palo Alto, CA). Amplification of cDNAs and suppression subtractive hybridization (SSH) followed the manufacturer's protocol for SSH (Clontech). PCR-amplified products resulting from two rounds of hybridization were cloned non-directionally into pCR2.1 (Invitrogen, Carlsbad, CA). Colony PCR using M13 forward and reverse primers was used to identify recombinant plasmids containing inserts >200 bp. Recombinant plasmid DNA was sequenced in both directions using Nested PCR primers 1 and 2R (Clontech) or M13 forward and reverse primers. The sequences obtained from the B Reverse library represent sequences downregulated during long day (16 h light/8 h dark) photoperiods at 25oC.

characteristic: description

text: current year's growth

text: E. coli DH5alpha

text: branch

text: Prunus persica

text: monoecious

text: bark (mostly cambium)

text: Subtraction suppressive hybridization library B Reverse

characteristic: title

text: plasmid

characteristic: vector


text: USDA, Agricultural Research Service

text: live

text: Generic

characteristic: cultivar

text: Total RNA was prepared from peach bark and used as a template for cDNA synthesis using the SuperSMART system (Clontech, Palo Alto, CA). Amplification of cDNAs and suppression subtractive hybridization (SSH) followed the manufacturer's protocol for SSH (Clontech). PCR-amplified products resulting from two rounds of hybridization were cloned non-directionally into pCR2.1 (Invitrogen, Carlsbad, CA). Colony PCR using M13 forward and reverse primers was used to identify recombinant plasmids containing inserts >200 bp. Recombinant plasmid DNA was sequenced in both directions using Nested PCR primers 1 and 2R (Clontech) or M13 forward and reverse primers. The sequences obtained from the C Forward library represent sequences upregulated during night break photoperiods at 5oC. Night break simulates a long day (16 h light/8 h dark) photoperiod, but the actual light exposure is equivalent to a short day (8 h light/16 h dark) exposure.

text: current year's growth

text: E. coli DH5alpha

characteristic: lab-host

text: branch

text: Prunus persica

text: monoecious

text: bark (mostly cambium)

characteristic: tissue-type

text: Subtraction suppressive hybridization library C Forward

text: plasmid


characteristic: External Id

text: USDA, Agricultural Research Service

characteristic: INSDC center name

text: live

text: Generic

text: Total RNA was prepared from peach bark and used as a template for cDNA synthesis using the SuperSMCRT system (Clontech, Palo Alto, CA). Amplification of cDNAs and suppression subtractive hybridization (SSH) followed the manufacturer's protocol for SSH (Clontech). PCR-amplified products resulting from two rounds of hybridization were cloned non-directionally into pCR2.1 (Invitrogen, Carlsbad, CA). Colony PCR using M13 forward and reverse primers was used to identify recombinant plasmids containing inserts >200 bp. Recombinant plasmid DNA was sequenced in both directions using Nested PCR primers 1 and 2R (Clontech) or M13 forward and reverse primers. The sequences obtained from the C Reverse library represent sequences downregulated during night break photoperiods at 5oC. Night break simulates a long day (16 h light/8 h dark), but the actual light exposure is equivalent to a short day (8 h light/16 h dark) photoperiod.

text: current year's growth

text: E. coli DH5alpha

text: branch

text: Prunus persica

text: monoecious

text: bark (mostly cambium)

text: Subtraction suppressive hybridization library C Reverse

text: plasmid


text: USDA, Agricultural Research Service

characteristic: INSDC first public

text: live

text: Generic

text: Total RNA was prepared from peach bark and used as a template for cDNA synthesis using the SuperSMART system (Clontech, Palo Alto, CA). Amplification of cDNAs and suppression subtractive hybridization (SSH) followed the manufacturer's protocol for SSH (Clontech). PCR-amplified products resulting from two rounds of hybridization were cloned non-directionally into pCR2.1 (Invitrogen, Carlsbad, CA). Colony PCR using M13 forward and reverse primers was used to identify recombinant plasmids containing inserts >200 bp. Recombinant plasmid DNA was sequenced in both directions using Nested PCR primers 1 and 2R (Clontech) or M13 forward and reverse primers. The sequences obtained from the E Forward library represent sequences upregulated at 5oC during exposure to short day (8 h light/16 h dark) photoperiods.

text: current year's growth

text: E. coli DH5alpha

text: branch

text: Prunus persica

text: monoecious

text: bark (mostly cambium)

text: Subtraction suppressive hybridization library E Forward

text: plasmid


text: USDA, Agricultural Research Service

text: live

text: Generic

text: Total RNA was prepared from peach bark and used as a template for cDNA synthesis using the SuperSMART system (Clontech, Palo Alto, CA). Amplification of cDNAs and suppression subtractive hybridization (SSH) followed the manufacturer's protocol for SSH (Clontech). PCR-amplified products resulting from two rounds of hybridization were cloned non-directionally into pCR2.1 (Invitrogen, Carlsbad, CA). Colony PCR using M13 forward and reverse primers was used to identify recombinant plasmids containing inserts >200 bp. Recombinant plasmid DNA was sequenced in both directions using Nested PCR primers 1 and 2R (Clontech) or M13 forward and reverse primers. The sequences obtained from the E Reverse library represent sequences downregulated at 5oC during exposure to short day (8 h light/16 h dark) photoperiods.

text: current year's growth

characteristic: dev-stage

text: E. coli DH5alpha

text: branch

text: Prunus persica

text: monoecious

text: bark (mostly cambium)

text: Subtraction suppressive hybridization library E Reverse

text: plasmid


text: University of Bologna

characteristic: INSDC last update

text: live

text: Generic

text: HcrVf2 trangenic Gala

text: Total RNA was extracted from frozen pooled leaf tissues of seven independent scab-resistant Gala lines transformed with the HcrVf2 gene (Belfanti et al., 2004) and the scab susceptible cultivar Gala challenged with Venturia inaequalis. Poly(A)+mRNA was isolated from total RNA using the FastTrack 2.0 kit (Invitrogen). The library was prepared using the PCR-Select Subtractive Hybridization Kit (Clontech). A 'forward' subtraction was performed to isolate fragments corresponding to genes whose expression level is increased in resistant genotypes during interaction with the pathogen. This was done by subtracting the cDNA prepared from the susceptible cv. Gala pooled leaves collected just after inoculum (used as 'driver') to the cDNA from Ga2 resistant pooled leaves collected at various times after inoculum (used as 'tester'). A 'reverse' subtraction was also carried out to isolate fragments corresponding to genes whose expression level is decreased following the onset of plant/pathogen interaction. Each type of subtraction was performed twice, starting from total RNA obtained during independent extractions. The ligated cDNA fragments were transformed into E.coli DH5 alpha host cells.

text: E. coli DH5alpha

text: Malus domestica

text: young leaves

text: Venturia inaequalis challenged cDNA suppression subtractive hybridization library

text: Plasmid

text: pGEM-T Easy


text: Broad University

text: live

characteristic: INSDC status

text: Generic

text: >230 adult and juvenile central ganglia; salivary glands; buccal, penal, pedal, body wall muscles; heart; all major parts of the reproductive, endocrine systems; digestive gland with tissues, all major embryonic and larval stages (>500 animals total.) Constructed at DNAform (Japan.)

text: Aplysia californica

text: XhoI/SalI

text: BamHI

text: Multiple

characteristic: strain

text: Aplysia californica Pooled Normalized Library

text: Plasmid

text: pFLCIII-cDNA


text: Washington University School of Medicine

text: live

text: Generic

characteristic: NCBI submission model

text: X

text: Caenorhabditis brenneri

text: BsrgI

text: BsrgI

text: plasmid


text: live

text: Generic

characteristic: NCBI submission package

text: DH10B cells

text: Caenorhabditis japonica

text: BsrgI

text: BsrgI

text: plasmid


text: live

text: Generic

text: The library was created by Irina Ronko at Washington University, St. Louis using Cloneminer cDNA library construction kit from Invitrogen. Size fractionation was done with columns provided.

text: DH10B cells

text: Caenorhabditis japonica

characteristic: organism

text: BsrgI

text: BsrgI

text: plasmid


text: live

text: Generic

text: Adult

text: Onchocerca flexuosa

text: BsrgI

text: BsrgI

text: Female

characteristic: sex

text: plasmid


text: live

text: Generic

text: Whole worm

text: Lucigen E.cloni 10G Supreme

text: Onchocerca flexuosa

text: HincII

text: HincII

text: Adult Female

text: plasmid

text: pSMART


text: live

text: Generic

text: Adult worm

text: Lucigen E.cloni 10G Supreme

text: Trichuris muris

text: HincII

text: HincII

text: plasmid

text: pSMART


text: live

text: Generic

text: Trichuris muris

text: BsrgI

text: BsrgI

text: plasmid


text: Faculty of Medicine, Chulalongkorn University

text: live

text: Generic

text: Daboia siamensis

text: EcoR I

text: Xho I

text: Russell's viper venom gland cDNA Library

text: bacteriophage

text: Lambda Zap III


text: British Antarctic Survey

text: live

text: Generic

text: Animals collected from Lagoon Island near Rothera Research Station, Adelaide Island, Antarctic Peninsula

characteristic: description

text: Cryptopygus antarcticus

text: whole animal

text: Cryptopygus antarcticus whole animal cDNA library


text: Institute of Himalayan Bioresource Technology

text: live

text: Generic

text: Leaf

characteristic: organ

text: Sinopodophyllum hexandrum

text: Leaf

text: Podophyllum hexandrum Royle suppression subtractive hybridization (SSH) library

characteristic: title

text: plasmid

text: pGEM-T Easy (Promega)


text: Kasetsart University

text: live

text: Generic

text: The cDNA library was generated as part of Oreochromis niloticus EST project. Total RNA was extracted from head kidney of Oreochromis niloticus infected with Streptococcus agalactiae. The mRNA was further purified, reverse transcribed and cloned into Uni-ZAP XR vector.

text: Oreochromis niloticus

text: EcoR I

text: Xho I

text: Head Kidney

text: Head kidney of Oreochromis niloticus infected with Streptococcus agalactiae

text: Phage

text: Uni-ZAP XR

characteristic: vector


text: Kasetsart University

text: live

text: Generic

text: The cDNA library was generated as part of Oreochromis niloticus EST project. Total RNA was extracted from spleen of Oreochromis niloticus infected with Streptococcus agalactiae. The mRNA was further purified, reverse transcribed and cloned into Uni-ZAP XR vector.

text: Oreochromis niloticus

text: EcoR I

text: Xho I

text: Spleen

text: Spleen of Oreochromis niloticus infected with Streptococcus agalactiae

text: Phage

text: Uni-ZAP XR


text: RIKEN Center for Developmental Biology

text: live

text: Generic

text: Pelodiscus sinensis

text: mixed

text: mixed tissues of liver, spleen and kidney

text: Chinese soft-shelled turtle cDNA library


text: Leibniz Institut fur Pflanzengenetik und Kulturpflanzenforschung (IPK)

text: live

text: Generic

text: Boechera cf. holboellii SvA-2008

text: 454 Generated sequence (FLX) normalized cDNA flower-specific transcriptome


text: IRTA

text: live

text: Generic

text: Organs and stages: Testis, ovary, undifferentiated gonads, brain, stomach, intestine, liver, whole larvae at premetamorphosis, metamorphosis and postmetamorphosis, and abnormal larvae. Normalised master library formed by a mixture of ten cDNA libraries from adult tissues and larvae. Each library was constructed in ZAP Express (Stratagene)

text: Adult and larvae

text: mixed

text: Solea senegalensis

text: XhoI

text: EcoRI

text: Male and females

text: Wild-caught fish

text: Solea senegalensis Mix of cDNA libraries

text: pBK-CMV


text: University of New Hampshire

text: live

text: Generic

text: Smooth Muscle

characteristic: cell type

text: Primary Culture, No Sub-Culture

characteristic: cell-line

text: One to three day-old atherosclerosis-susceptible (spontaneous, non-induced) White Carneau (WC) and atherosclerosis-resistant Show Racer (SR) pigeons were used to prepare primary cultures of aortic smooth muscle cells (SMC). Explants were grown for seven days until they formed a confluent monolayer. Total RNA was extracted (Trizol, Invitrogen) and converted to double-stranded cDNA using the BD SMART PCR cDNA Synthesis Kit (Clontech). Full-length cDNA from each pigeon strain was cut with DpnII (5'GATC) in order to prepare amplicons for comparison by Representational Difference Analysis (RDA). cDNA fragments were subjected to three rounds of PCR-coupled subtractive hybridization and the final difference products were cloned into the BamHI restriction site of the pBluescript SK+ phagemid vector and transformed into XL1 MRF- chemically competent host cells (Stratagene). Transcripts were purified from the host cells by Rolling Circle Amplification (GE Healthcare) and sequenced by the DNAcore facility at the Hubbard Center for Genome Studies, University of New Hampshire. Sequences represent genes that were differentially expressed between the two strains. The WC136 library contains transcripts that were upregulated in WC SMC relative to SR. The corresponding SR library (SR136) contains transcripts that were up regulated in the SR SMC relative to WC136.

text: XL1 MRF-

characteristic: lab-host

text: Columba livia

text: BAMHI

text: Mixed

text: White Carneau

text: Descending Aorta

text: pBluescript SK+


characteristic: External Id

text: University of California, Santa Cruz

characteristic: INSDC center name

text: live

text: Generic

text: Adult

text: E. coli XL1-Blue

text: Mytilus californianus

text: Male & Female

text: Gill & Mantle & Adductor muscle

characteristic: tissue-type

text: Mytilus californianus cDNA library

text: Phage

text: Lambda ZAP II


text: Northwest A&F University

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

characteristic: cultivar

text: first leaf of seven-day old wheat seedlings

characteristic: dev-stage

text: Triticum aestivum

text: leaf

text: Transcript derived fragments (TDFs) from wheat leaves inoculated by stripe rust fungus

text: pGEM-T easy


text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Solanum lycopersicum

text: leaf

text: Tomato leave Library


text: University of Melbourne

text: live

text: Generic

text: Oxyrrhis marina

characteristic: organism

text: CCMP 1788

characteristic: strain

text: Oxyrrhis marina CCMP 1788


text: National Institute of Genetics

text: live

text: Generic

text: A Lambda ZAP II cDNA library was constructed with mRNA extracted from young panicles (ca 2-4 cm long) of Oryza punctata (W1514) using ZAP-cDNA Synthesis Kit and ZAP-cDNA Gigapack III Gold Cloning Kit (Stratagene) and converted to pBluescript SK- (amp resistant) sub-libraries. For insert amplification, M13 forward and reverse primers can be used.

characteristic: description

text: 4th leaf stage

text: Oryza punctata

text: EcoRI

text: XhoI

text: shoot apical meristem

text: shoot apical meristem of BB genome(BBS)

characteristic: title

text: Lambda ZAP II/pBluescript SK-


text: National Institute of Genetics

text: live

text: Generic

text: A Lambda ZAP II cDNA library was constructed with mRNA extracted from shoot meristem tissue of Oryza officinalis (W0002) using ZAP-cDNA Synthesis Kit and ZAP-cDNA Gigapack III Gold Cloning Kit (Stratagene) and converted to pBluescript SK- (amp resistant) sub-libraries. For insert amplification, M13 forward and reverse primers can be used.

text: early panicle development stage

text: Oryza officinalis

text: EcoRI

text: XhoI

text: whole panicle

text: 2-4 cm panicle of CC genome(CCP)

text: Lambda ZAP II/pBluescript SK-

characteristic: vector


text: National Institute of Agrobiological Sciences

text: live

text: Generic

text: 5 weeks

text: Sus scrofa

text: male

characteristic: sex

text: main and accessory cerebral

text: full-length enriched pig cDNA library, main and accessory cerebral


text: live

text: Generic

text: main and accessory cerebral lymphocyte

characteristic: cell type

text: 5 weeks

text: Sus scrofa

text: male

text: full-length enriched pig cDNA library, main and accessory cerebral lymphocyte


characteristic: External Id

text: Universite de Pau et des Pays de l'Adour

text: live

text: Generic

text: isolation_source: pristine microbial mat contaminated in microcosm experiments by heavy fuel oil; environmental_sample; country: France:South East

text: uncultured Geobacter sp.

text: uncultured Geobacter sp. library (Bordenave S)


characteristic: INSDC center name

text: live

text: Generic

text: uncultured Pelotomaculum sp.

text: uncultured Pelotomaculum sp. library (Bordenave S)


text: live

text: Generic

text: uncultured Xanthomonas sp.

text: uncultured Xanthomonas sp. library (Bordenave S)


text: live

text: Generic

text: uncultured Mannheimia sp.

text: uncultured Mannheimia sp. library (Bordenave S)


text: live

text: Generic

text: uncultured Arthrobacter sp.

text: uncultured Arthrobacter sp. library (Bordenave S)


text: live

text: Generic

text: isolation_source: control pristine microbial mat in microcosm experiments; environmental_sample; country: France:South East

text: uncultured bacterium

text: uncultured bacterium library (Bordenave S)


text: Shimane University

text: live

text: Generic

text: Saijo

text: pre-flowering to post-flowering stages

text: Diospyros kaki

text: ovary and young fruit

characteristic: tissue-type

text: Diospyros kaki ovary and young fruit pre-flowering to post-flowering stages


text: Shimane University

characteristic: INSDC first public

text: live

text: Generic

text: Saijo

text: Diospyros kaki

text: mature fruit

text: Diospyros kaki mature fruit initiation of color change to over-softening stages


text: National Agricultural Research Center for Hokkaido Region

characteristic: INSDC last update

text: live

text: Generic

text: Aberystwyth S23

text: Lolium perenne

text: crown

text: perennial ryegrass cold-acclimated plants cDNA


text: RIKEN BioResource Center

text: live

text: Generic

text: 2 weeks old

text: Brassica rapa subsp. pekinensis

text: aerial part

text: Chinese cabbage cDNA library, 2 weeks old aerial part


text: Zhejiang University

text: live

text: Generic

text: Jinchun No.4

characteristic: cultivar

text: Growing stage

text: E.coli DH5a

characteristic: lab-host

text: Cucumis sativus

text: EcoRI

text: Root

text: Root cDNA library of cucumber (Cucumis sativus)

text: Plasmid

text: pGEM-T easy


text: Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences

text: live

characteristic: INSDC status

text: Generic

text: Cyprinus carpio

text: Brain

text: Carp brain Suppression subtractive hybridization cDNA Library

text: Plasmid

text: pMD 18 T-vector


text: USDA-ARS FRRL

text: live

text: Generic

characteristic: NCBI submission model

text: The library was constructed and normalized as described by Bonaldo, M.F., Lennon, G. and Soares, M.B. (1996), Genome Research 6(9): 791-806. An identifying tag was added at the 3'during cDNA synthesis: insertAAAAAAAAAAAAAAAAAATGCGT (for shoot clones) or insertAAAAAAAAAAAAAAAAAATCGGT (for root clones) or insertAAAAAAAAAAAAAAAAAATGCGA (for seedling clones).

text: Pseudoroegneria spicata

text: EcoRI (5' side of insert)

text: NotI (3' side of insert)

text: etiolated seedlings, salt and drought stressed roots, and salt and drought stressed shoots and crowns

text: BG02 - primary and normalized libraries

text: Plasmid

text: pBS II SK(+)


text: Max Planck Institute for Chemical Ecology

text: live

text: Generic

characteristic: NCBI submission package

text: Normalized cDNA library generated from induced, immunized, plant-fed larvae

text: larval instars 1-5

characteristic: dev-stage

text: Trichoplusia ni

text: mixed

text: mixed

text: Trichoplusia ni whole larvae normalized cDNA library


text: live

text: Generic

text: Non-normalized larval cDNA library generated from plant-fed larvae.

text: larval instars 1-5

text: Trichoplusia ni

characteristic: organism

text: mixed

text: mixed

text: Trichoplusia ni larval non-normalized cDNA library


text: University of New Hampshire

text: live

text: Generic

text: Smooth Muscle

text: Primary Culture, No Sub-Culture

characteristic: cell-line

text: One to three day-old atherosclerosis-susceptible (spontaneous, non-induced) White Carneau (WC) and atherosclerosis-resistant Show Racer (SR) pigeons were used to prepare primary cultures of aortic smooth muscle cells (SMC). Explants were grown for seven days until they formed a confluent monolayer. Total RNA was extracted (Trizol, Invitrogen) and converted to double-stranded cDNA using the BD SMART PCR cDNA Synthesis Kit (Clontech). Full-length cDNA from each pigeon strain was cut with DpnII (5'GATC) in order to prepare amplicons for comparison by Representational Difference Analysis (RDA). cDNA fragments were subjected to three rounds of PCR-coupled subtractive hybridization and the final difference products were cloned into the BamHI restriction site of the pBluescript SK+ phagemid vector and transformed into XL1 MRF- chemically competent host cells (Stratagene). Transcripts were purified from the host cells by Rolling Circle Amplification (GE Healthcare) and sequenced by the DNAcore facility at the Hubbard Center for Genome Studies, University of New Hampshire. Sequences represent genes that were differentially expressed between the two strains. The WC143 library contains transcripts that were upregulated in WC SMC relative to SR. The corresponding SR library (SR143) contains transcripts that were up regulated in the SR SMC relative to WC143.

characteristic: description

text: XL1 MRF-

text: Columba livia

text: BAMHI

text: Mixed

text: White Carneau

characteristic: strain

text: Descending Aorta

text: pBluescript SK+


text: The International Institute of Tropical Agriculture

text: live

text: Generic

text: Total RNA was isolated from pools of leaf and stem meristem tissue using Invitrogen's concert RNA reagent following manufacturer's protocol. The pools of tissue comprised equal weights of tissue from each of: drought stressed and non-stressed cassava lines, Sauti, Gomani, Mbundumali, TME 1 and Mkondezi. A single total RNA sample was derived by combining RNAs from leaf and stem meristem on a 1:1 quantity basis. Reverse transcription and cDNA normalization was conducted at EVROGEN using SMART technology. Directional library was constructed at TIGR using a creator SMART cDNA library construction kit from BD Clontech

text: mature, pre flowering

text: Manihot esculenta

text: SfiI

text: leaf and stem meristem

text: CASL

characteristic: title

text: pDNR-LIB

characteristic: vector


characteristic: External Id

text: live

text: Generic

text: Dan Ila, Tvu11986, Vu7778 and 12008D

text: Total RNA was isolated from pools of root tissue using Invitrogen's concert RNA reagent following manufacturer's protocol. The pools of tissue comprised equal weights of tissue from each of: drought stressed and non-stressed cowpea lines Dan Ila, Tvu11986, Vu7778 and 12008D. Reverse transcription and cDNA normalization was conducted at EVROGEN using SMART technology. Directional library was constructed at TIGR using a creator SMART cDNA library construction kit from BD Clontech.

text: mature, pre flowering

text: Vigna unguiculata

text: SfiI

text: root

text: MOO

text: pDNR-LIB


text: Huazhong Agricultural University

characteristic: INSDC center name

text: live

text: Generic

text: protoplast cultured for 0-48hours

text: Gossypium hirsutum

text: Coker 201

text: protoplast

text: suppression subtractive hybridization library of cell wall regeneration from protoplast


text: School of Life Sciences, Xiamen University

text: live

text: Generic

text: Female Sebastiscus marmoratus weighing 23-27 g, were captured from coastal area in Xiamen city, Fujian province, China. Before the exposure experiment, the fish were acclimated in tanks (25 fish in each tank) containing 50 L of aerated sand-filtered seawater, with natural photoperiod for 7 days. Fish were dosed via single intraperitoneal injection with PCBs (3 mg/kg body weight in corn oil) or with corn oil as vehicle controls. After injection for 3 days, fish were randomly sampled from each group. The isolated brains were frozen in liquid N2 immediately stored at -80o until analyzed. For the SSH procedure, mRNA was isolated from brains and two libraries constructed in pMD18-T vector, enriched for genes up- (forward subtracted) or down-regulated (reverse subtracted), respectively in response to PCBs.

text: Adult

text: E.coli TOP10

text: Brain

characteristic: organ

text: Sebastiscus marmoratus

text: female

characteristic: sex

text: Suppression subtractive hybridization libraries for brain of Sebastiscus marmoratus in response to PCBs

text: Plasmid


text: Aristotle University of Thessaloniki

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Produced with Creator Smart cDNA Library Construction Kit

text: Cistus creticus subsp. creticus

text: SfiA

text: SfiB

text: trichomes

characteristic: tissue-type

text: Cistus creticus trichome library, Clontech

text: Phage

text: lTriplex 2


text: NRC Institute for Marine Biosciences Industrial Partnership Facility

text: live

characteristic: INSDC status

text: Generic

text: Gadus morhua

text: head kidney

text: Gadus morhua head kidney library (injected with formalin-killed Aeromonas salmonicida)


text: live

text: Generic

characteristic: NCBI submission model

text: Gadus morhua

text: blood

text: Gadus morhua blood SSH library enriched for genes up-regulated by polyriboinosinic polyribocytidylic acid (polyI:C)


text: live

text: Generic

characteristic: NCBI submission package

text: Gadus morhua

text: blood

text: Gadus morhua blood SSH library enriched for genes down-regulated by polyriboinosinic polyribocytidylic acid (polyI:C)


text: Nanjing Agricultural University

text: live

text: Generic

text: Sus scrofa

text: intramuscular fat

text: Sus scrofa localization of cDNA clones from intramuscular fat library by cDNA-AFLP


text: National Institute of Animal Science, Rural Development Administration

text: live

text: Generic

text: Secale cereale


text: live

text: Generic

text: Pisang Awak (ABB), Sukari Ndizi (AB), Mpologoma (AAA)

characteristic: cultivar

text: Total RNA was isolated from pools of leaf and stem meristem tissue using Invitrogen's concert RNA reagent following manufacturer's protocol. The pools of tissue comprised equal weights of tissue from each of: drought stressed and non-stressed banana cultivars Pisang Awak (ABB), Sukari Ndizi (AB) and Mpologoma (AAA). A single total RNA sample was derived by combining RNAs from leaf and stem meristem on a 1:1 quantity basis. Reverse transcription and cDNA normalization was conducted at EVROGEN using SMART technology. Directional library was constructed at TIGR using a creator SMART cDNA library construction kit from BD Clontech.

text: juvenile, pre flowering

characteristic: dev-stage

text: Musa acuminata

text: SfiI

text: leaf and shoot meristem

text: MUSL

text: pDNR-LIB


text: The International Institute of Tropical Agriculture

text: live

text: Generic

text: Total RNA was isolated from pools of root tissue using Invitrogen's concert RNA reagent following manufacturer's protocol. The pools of tissue comprised equal weights of tissue from each of: drought stressed and non-stressed banana cultivars Pisang Awak (ABB), Sukari Ndizi (AB) and Mpologoma (AAA). Reverse transcription and cDNA normalization was conducted at EVROGEN using SMART technology. Directional library was constructed at TIGR using a creator SMART cDNA library construction kit from BD Clontech.

text: juvenile, pre flowering

text: Musa acuminata

characteristic: organism

text: SfiI

text: root

text: MUSR

text: pDNR-LIB


text: live

text: Generic

text: Dan Ila, Tvu11986, Vu7778 and 12008D

text: Total RNA was isolated from pools of leaf and stem meristem tissue using Invitrogen's concert RNA reagent following manufacturer's protocol. The pools of tissue comprised equal weights of tissue from each of: drought stressed and non-stressed cowpea lines Dan Ila, Tvu11986, Vu7778 and 12008D. A single total RNA sample was derived by combining RNAs from leaf and stem meristem on a 1:1 quantity basis. Reverse transcription and cDNA normalization was conducted at EVROGEN using SMART technology. Directional library was constructed at TIGR using a creator SMART cDNA library construction kit from BD Clontech.

characteristic: description

text: mature, pre flowering

text: Vigna unguiculata

text: SfiI

text: leaf and shoot meristem

text: MOP

text: pDNR-LIB


text: live

text: Generic

text: Total RNA was isolated from pools of root tissue using Invitrogen's concert RNA reagent following manufacturer's protocol. The pools of tissue comprised equal weights of tissue from each of: drought stressed and non-stressed cassava lines, Sauti, Gomani, Mbundumali, TME 1 and Mkondezi. Reverse transcription and cDNA normalization was conducted at EVROGEN using SMART technology. Directional library was constructed at TIGR using a creator SMART cDNA library construction kit from BD Clontech.

text: mature, pre flowering

text: Manihot esculenta

text: SfiI

text: root

text: CASR

text: pDNR-LIB


text: J. Craig Venter Institute (JCVI; www.jcvi.org)

text: live

text: Generic

text: Cells (strain CU428) were grown to exponential phase in rich (SPP) medium and harvested.

text: Tetrahymena thermophila

characteristic: strain

text: Tetrahymena thermophila CU428 log phase, rich medium cDNA library


text: Instituto de Investigaciones Marinas (IIm), CSIC

text: live

text: Generic

text: Dicentrarchus labrax

text: Head Kidney

text: Nodavirus infected sea bass SSH library

characteristic: title


text: INTA - University of Chile

text: live

text: Generic

text: 6-7 stage embryo according with Campos-Ortega and Harstenstein, 1985

text: Drosophila melanogaster

text: CantonS

text: Drosophila gastrula substracted cDNA library

text: pGEMT-Easy

characteristic: vector


characteristic: External Id

text: USDA-ARS FRRL

text: live

text: Generic

text: The library was constructed and normalized as described by Bonaldo, M.F., Lennon, G. and Soares, M.B. (1996), Genome Research 6(9): 791-806. An identiying tag was added at the 5'during cDNA synthesis: ACGGCCGGG-insert (for Elymus lanceolatus clones) or ACGGCCGAGGG-insert (for Elymus wawawaiensis clones).

characteristic: lab-host

text: Elymus wawawaiensis/Elymus lanceolatus mixed EST library

text: EcoRI (5' side of insert)

text: NotI (3' side of insert)

text: Subterranean tiller (Elymus wawawaiensis ) or tiller/rhizome (Elymus lanceolatus) meristems

text: BG03- primary and normalized libraries

text: Plasmid

text: pBS II SK(+)


text: DOE Joint Genome Institute

characteristic: INSDC center name

text: live

text: Generic

text: The library was made from dT primed cDNA and cloned into the pDONR222 vector (Invitrogen Corp;, Carlsbad, CA). PolyA RNA was primed with a Biotin-attB2-oligo-dT primer (5'-GGCGGCCGCACAACTTTGTACAAGAAAGTTGGGT(T)19-3') and the first strand synthesized using Superscript II (Invitrogen Corp;, Carlsbad, CA). The second strand was synthesized and an attB1 adaptor was ligated to the 5' end (5'-TCGTCGGGGACAACTTTGTACAAAAAAGTTGG-3' and 5'-CCAACTTTTTTGTACAAAGTTGTCCC -3'). cDNA was inserted into the vector using site specific recombination (flanking attB sites on cDNA). Library was constructed in the laboratory of P. Lemaire at Developmental Biology Institute of Marseille and sequencing was performed by the DOE Joint Genome Institute (Walnut Creek, CA).

text: mature adult

text: DH10B TonA

text: Ciona intestinalis

text: whole animal

text: Yutaka Satou unpublished cDNA library, mature adult whole animal


text: Harvard Medical School

text: live

text: Generic

text: Normalized library, G708 and G825 (gastrula). cDNA inserts cloned into NotI/EcoRV sites of pExpress1 vector (Express Genomics). 5' end of insert at EcoRV site of vector.

text: gastrula

text: Saccoglossus kowalevskii

text: NotI

text: EcoRV

text: Acorn worm normalized gastrula pExpress1 library

text: plasmid


text: Harvard Medical School

text: live

text: Generic

text: Normalized library, G710 and G826 (juvenile). cDNA inserts cloned into NotI/EcoRV sites of pExpress1 vector (Express Genomics). 5' end of insert at EcoRV site of vector.

text: juvenile

text: Saccoglossus kowalevskii

text: NotI

text: EcoRV

text: Acorn worm normalized juvenile pExpress1 library

text: plasmid


text: National Institute of Biomedical Innovation

text: live

text: Generic

text: Cap structure of mRNA may be replaced by guanine (G). This library was made using Vector-capping method. See, Kato, S. et al. (2005) Vector-capping: a simple method for preparing a high-quality Full-Length cDNA Library. DNA Res. 12:53-62.

text: 3 years old

text: Macaca fascicularis

text: Swa I

text: Not I

text: male

characteristic: sex

text: liver

text: macaque liver cDNA library QlvC


text: Kyoto Prefectural University of Medicine

characteristic: INSDC first public

text: live

text: Generic

text: corneal epithelial cells

characteristic: cell type

characteristic: cell-line

text: adult

text: Homo sapiens

text: human cDNA library, immortalized cell line of corneal epithelial cells


text: The Breed & Biotechnology Key Laboratory of Sheep

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: immature proglottid of adult

text: Moniezia expansa

text: Shihezi

text: Moniezia expansa Shihezi immature proglottids


text: Hefei Teachers College

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Phytophthora sojae

text: Phytophthora sojae adaption express library


text: University of New Hampshire

text: live

text: Generic

text: Smooth Muscle

text: Primary Culture, No Sub-Culture

text: One to three day-old atherosclerosis-susceptible (spontaneous, non-induced) White Carneau (WC) and atherosclerosis-resistant Show Racer (SR) pigeons were used to prepare primary cultures of aortic smooth muscle cells (SMC). Explants were grown for seven days until they formed a confluent monolayer. Total RNA was extracted (Trizol, Invitrogen) and converted to double-stranded cDNA using the BD SMART PCR cDNA Synthesis Kit (Clontech). Full-length cDNA from each pigeon strain was cut with DpnII (5'GATC) in order to prepare amplicons for comparison by Representational Difference Analysis (RDA). cDNA fragments were subjected to three rounds of PCR-coupled subtractive hybridization and the final difference products were cloned into the BamHI restriction site of the pBluescript SK+ phagemid vector and transformed into XL1 MRF- chemically competent host cells (Stratagene). Transcripts were purified from the host cells by Rolling Circle Amplification (GE Healthcare) and sequenced by the DNAcore facility at the Hubbard Center for Genome Studies, University of New Hampshire. Sequences represent genes that were differentially expressed between the two strains. The WC224 library contains transcripts that were upregulated in WC SMC relative to SR. The corresponding SR library (SR224) contains transcripts that were up regulated in the SR SMC relative to WC224.

text: XL1 MRF-

text: Columba livia

text: BAMHI

text: Mixed

text: White Carneau

text: Descending Aorta

characteristic: tissue-type

text: pBluescript SK+


text: University of New Hampshire

text: live

text: Generic

text: Smooth Muscle

text: Primary Culture, No Sub-Culture

text: One to three day-old atherosclerosis-susceptible (spontaneous, non-induced) White Carneau (WC) and atherosclerosis-resistant Show Racer (SR) pigeons were used to prepare primary cultures of aortic smooth muscle cells (SMC). Explants were grown for seven days until they formed a confluent monolayer. Total RNA was extracted (Trizol, Invitrogen), purified, and converted to double-stranded cDNA using the BD SMART PCR cDNA Synthesis Kit (Clontech). Full-length cDNA from each pigeon strain was cut with DpnII (5'GATC) in order to prepare amplicons for comparison by Representational Difference Analysis (RDA). cDNA fragments were subjected to three rounds of PCR-coupled subtractive hybridization and the final difference products were cloned into the BamHI restriction site of the pBluescript SK+ phagemid vector and transformed into XL1 MRF- chemically competent host cells (Stratagene). Transcripts were purified from the host cells by Rolling Circle Amplification (GE Healthcare) and sequenced by the DNAcore facility at the Hubbard Center for Genome Studies, University of New Hampshire. Sequences represent genes that were differentially expressed between the two strains. The WC225 library contains transcripts that were upregulated in WC SMC relative to SR. The corresponding SR library (SR225) contains transcripts that were upregulated in the SR SMC relative to WC225.

text: XL1 MRF-

text: Columba livia

characteristic: organism

text: BAMHI

text: Mixed

text: White Carneau

text: Descending Aorta

text: pBluescript SK+


text: Washington University School of Medicine

text: live

text: Generic

text: G10 Gene Supreme

text: Heterorhabditis bacteriophora

text: HincII

text: HincII

text: Heterorhabditis_bacteriophora_EST_Infective_Juvenile_pSMART

text: plasmid

text: pSMART-cDNA


text: live

text: Generic

text: G10 Gene Supreme

text: Heterorhabditis bacteriophora

text: HincII

text: HincII

text: Heterorhabditis_bacteriophora_EST_L1+L2_pSMART

text: plasmid

text: pSMART-cDNA


text: live

text: Generic

text: G10 Gene Supreme

text: Heterorhabditis bacteriophora

text: HincII

text: HincII

text: Heterorhabditis_bacteriophora_EST_L3+L4_pSMART

text: plasmid

text: pSMART-cDNA


text: live

text: Generic

text: G10 Gene Supreme

text: Heterorhabditis bacteriophora

text: HincII

text: HincII

text: Heterorhabditis_bacteriophora_EST_L4+baggedworms_pSMART

text: plasmid

text: pSMART-cDNA


text: University of Cordoba

text: live

text: Generic

text: Subtractive hybridization was performed with the PCR-Select cDNA Subtraction Kit (Clontech), according to the manufacture's protocol. Tester tissue was liver of fish that have been injected with PBS. Driver tissue consisted of liver of fish that have been treated with CuSO4 (2 mg/kg) for 6 and 24h.

characteristic: description

text: Liver

characteristic: organ

text: Solea senegalensis

text: Mixed

text: Senegalese sole CuSO4 down-regulated liver library

text: pCR2.1-TOPO (Invitrogen)


text: University of Cordoba

text: live

text: Generic

text: Subtractive hybridization was performed with the PCR-Select cDNA Subtraction Kit (Clontech), according to the manufacture's protocol. Tester tissue consisted of liver of fish that have been treated with CuSO4 (2 mg/kg) for 6 and 24h. Driver tissue was liver of fish that have been injected with PBS.

text: Liver

text: Solea senegalensis

text: Mixed

text: Senegalese sole CuSO4 up-regulated liver library

characteristic: title

text: pCR2.1-TOPO (Invitrogen)


text: University of Cordoba

text: live

text: Generic

text: Subtractive hybridization was performed with the PCR-Select cDNA Subtraction Kit (Clontech), according to the manufacture's protocol. Tester tissue was head kidney of fish that have been injected with PBS. Driver tissue consisted of head kidney of fish that have been treated with LPS (20 mg/kg) for 6 and 24h.

text: Head Kidney

text: Solea senegalensis

text: Mixed

text: Senegalese sole LPS down-regulated head kidney library

text: pCR2.1-TOPO (Invitrogen)

characteristic: vector


characteristic: External Id

text: University of Cordoba

text: live

text: Generic

text: Subtractive hybridization was performed with the PCR-Select cDNA Subtraction Kit (Clontech), according to the manufacture's protocol. Tester tissue consisted of head kidney of fish that have been treated with LPS (20 mg/kg) for 6 and 24h. Driver tissue was kidney of fish that have been injected with PBS.

text: Head Kidney

text: Solea senegalensis

text: Mixed

text: Senegalese sole LPS up-regulated head kidney library

text: pCR2.1-TOPO (Invitrogen)


text: National Tsing Hua University

text: live

text: Generic

text: Vigna radiata

text: Vigna radiata library (Lin TY)


text: Lanzhou Veterinary Research Institute

text: live

text: Generic

text: Peripheral Blood Mononuclear Cell

text: Bos taurus

text: male

text: Blood

text: Bos taurus Peripheral Blood Mononuclear Cell (PBMC) SSH cDNA Libraries


text: USDA-ARS-BARC

text: live

text: Generic

text: Merton Thornless

characteristic: cultivar

text: Rubus F2 backcross to F1 of R. ulmifolius (R. rusticanus) inermis x R. thyrsiger. The tetraploid blackberry cultivar, Merton Thornless (PI 553276), was selected for EST development, because it is the source of the thornless trait in many commercially grown thornless cultivars. Young expanding leaves of 'Merton Thornless' were harvested in July, 2004, from plants growing on the North Farm of the Beltsville Agricultural Research Center. No enrichment was done of the total RNA extracted before mRNA was separated from total RNA. The cDNA library was cloned in the Gateway system (Invitrogen Corp., Carlsbad, Calif.) with the pDONR222 vector and ElectroMAX DH10B T1 Phage Resistant Cells (Invitrogen Corp.). Kanamycin was used to select colonies containing vectors. The M13F primer was used to sequence from the 5' end of 3884 clones.

text: Newly emerging

characteristic: dev-stage

text: DH 10B

characteristic: lab-host

text: Rubus ulmifolius var. inermis x Rubus thyrsiger

text: sfiI

text: sfiII

text: Leaf

text: RH_MEa blackberry emerging leaf

text: Plasmid


text: New Mexico State University

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: 10 days after germination

text: Rhizophagus intraradices

text: germinating spore tissue

text: Glomus intraradices germinating spore tissue 10 days after germination


text: The Kansai Electric Power Co.

text: live

text: Generic

text: Chlamydomonas sp. W80

characteristic: strain

text: Chlamydomonas sp. W80 lambda ZAP cDNA library

text: lambda ZAP


text: DOE Joint Genome Institute

characteristic: INSDC last update

text: live

text: Generic

text: The library was made from dT primed cDNA and cloned into the pDONR222 vector (Invitrogen Corp;, Carlsbad, CA). PolyA RNA was primed with a Biotin-attB2-oligo-dT primer (5'-GGCGGCCGCACAACTTTGTACAAGAAAGTTGGGT(T)19-3') and the first strand synthesized using Superscript II (Invitrogen Corp;, Carlsbad, CA). The second strand was synthesized and an attB1 adaptor was ligated to the 5' end (5'-TCGTCGGGGACAACTTTGTACAAAAAAGTTGG-3' and 5'-CCAACTTTTTTGTACAAAGTTGTCCC -3'). cDNA was inserted into the vector using site specific recombination (flanking attB sites on cDNA). Library was constructed in the laboratory of P. Lemaire at Developmental Biology Institute of Marseille and sequencing was performed by the DOE Joint Genome Institute (Walnut Creek, CA).

text: cleavage to neurula

text: DH10B TonA

text: Ciona intestinalis

text: Roscoff

text: embryos

text: Gateway compatible cien cDNA library, Ciona intestinalis mixed embryonic stages (Egg to Neurula)


text: Hokkaido University

text: live

characteristic: INSDC status

text: Generic

text: Ultra

text: Preemergent proteoid root zones of Lupinus albus (white lupin) grown under phosphate deficient conditions were harvested, and poly(A)+ RNA was isolated. Seven micrograms of poly(A)+ RNA obtained was used for construction of an early proteoid root cDNA library in the phage ZAPII vector according to the manufacturer's instructions (Stratagene, La Jolla, CA, USA). For conversion of the phage library into the plasmid form, mass excision was performed according to the procedure described by Stratagene. All sequences were searched for vector segments to check for insert integrity. The sequences contain a minimum of 90 high quality bases and have been trimmed to remove vector and adaptor sequences.

text: preemergent proteoid root zones, phosphate-stressed

text: SOLR

text: preemergent proteoid roots, phosphate-stressed (cluster root)

text: Lupinus albus

text: EcoRI

text: XhoI

text: Preemergent cluster root ESTs derived from phosphate-stressed Lupinus Albus

text: pBluescript SK(+/-)


text: Chinese Academy of Agricultural Sciences Institute of Plant Protection

text: live

text: Generic

characteristic: NCBI submission model

text: Gliocladium catenulatum parasited on sclerotia of Sclerotinia sclerotiorum suppression subtractive hybridization library

text: Clonostachys rosea f. catenulata

text: Gliocladium catenulatum parasited on sclerotia of Sclerotinia sclerotiorum SSH library


text: Shanghai Institute of Veterinary Research, Chinese Academy of Agricultural Sciences

text: live

text: Generic

characteristic: NCBI submission package

text: Total RNA was extracted from 7d schistosomula of Schistosoma japonicum and 42d adult schistosoma worms by Trizol reagent. The EST sequenced subtractive library of 7day hepatic schistosomulum. This was performed using the SuperSmart PCR cDNA Synthesis kit and PCR-selected cDNA Subtraction kit (Clontech) according to the manufacturer's protocols. Briefly, 1ug of total RNA from 7d schistosomula was used in reverse transcription with a specific oligo(dT) primer, and double-stranded cDNAs were synthesized by 18 cycles. SMART cDNA from adult mixed sex schistosome (Driver) and the schistosomula (Tester) was digested with Rsa I to generate short, blunt-ended double-strand cDNA. After phenol extraction and ethanol precipitation, Rsa I-digested Tester was ligated with Adaptor I and Adaptor2 at 5'end of each strand of the tester cDNA. The adaptor 1-ligated and the adaptor 2-ligated tester cDNAs were then separately hybridized with an excess of dr iver cDNA at 68oC for 8h after denaturation at 98oC for 90s. The two hybridization samples were mixed together without denaturation and hybridized at 68oC overnight with an excess of fresh denatured driver cDNA. The resulting mixture was diluted in 200 ul dilution buffer followed by two PCRs. In the primary PCR, primers against adaptor 1 and adaptor 2 were used to selectively amplify differentially expressed cDNA. And then the product of the first PCR was used as template in the second PCR with nested primers. The final PCR product was cloned into the pGEM-T vector (Promega) with blue and white selection in E.coli DH5a.

text: 7d schistosomula

text: Schistosoma japonicum

text: Chinese mainland strain

text: Schistosoma japonicum 7d schistosomula subtractive expressed sequence tags library

text: promega T easy vector


text: INRS-Institut Armand-Frappier

text: live

text: Generic

text: Total RNA was isolated from either treated with thyroid hormone (T3), sodium ipodate (thyroid hormone inhibitor), or control group fish liver. The differential display polymerase chain reaction (DD-PCR) was done and differentially displayed bands were identified and excised from the gel. The purified cDNAs were ligated into the PCR 2.1-TOPO vector. Plasmids containing the cDNA inserts were then extracted and digested with the appropriate restriction enzyme (EcoRI, BamhI or EcoRV).

text: immature (1 year of age)

text: Salvelinus fontinalis

text: liver

characteristic: tissue-type

text: Thyroid hormone dependent genes in brook charr liver


text: University of Arizona

text: live

text: Generic

text: Gene specific primers used to amplify gene fragments from 1st strand cDNA

text: Immature

text: Zea mays

characteristic: organism

text: Tassels

text: Direct sequencing of RT-PCR products of Zea mays


text: Suzhou University

text: live

text: Generic

text: Neopyropia yezoensis

text: Porphyra yezoensis cDNA


text: Center for the Biology of Nematode Parasitism, North Carolina State University

text: live

text: Generic

text: Hicks Broadleaf

text: senescent leaf

text: Nicotiana tabacum

text: senescent leaf

text: Plasmid


text: live

text: Generic

text: Hicks Broadleaf

text: axillary bud

text: Nicotiana tabacum

text: axillary bud

text: Plasmid


text: live

text: Generic

text: Hicks Broadleaf

text: flower

text: Nicotiana tabacum

text: flower

text: Plasmid


text: live

text: Generic

text: Hicks Broadleaf

text: young plant

text: Nicotiana tabacum

text: young plant

text: Plasmid


text: Malaysian Palm Oil Board

text: live

text: Generic

text: Total RNA was isolated from oil palm embryogenic callus using the aqueous phenol extraction method. After total RNA extraction, poly (A)+ RNA was isolated using oligo-dT cellulose chromatography technique. Directional cDNA library were constructed using the ZAP-cDNA Synthesis Kit and the ZAP-cDNA Gigapack III Gold Cloning Kit (Stratagene). Oil palm embryogenic callus cDNAs were cloned and amplified once to produce a library with a titre of 6.10 X 10^9 pfu/ml.

characteristic: description

text: Embryogenic

text: Elaeis guineensis

text: EcoRI

text: XhoI

text: Callus

text: CEO (Oil Palm Embryogenic Callus)

text: Phagemid


text: Malaysian Palm Oil Board

text: live

text: Generic

text: Total RNA was isolated from oil palm non-embryogenic callus using the aqueous phenol extraction method. After total RNA extraction, poly (A)+ RNA was isolated using oligo-dT cellulose chromatography technique. Directional cDNA library were constructed using the ZAP-cDNA Synthesis Kit and the ZAP-cDNA Gigapack III Gold Cloning Kit (Stratagene). Oil palm non-embryogenic callus cDNAs were cloned and amplified once to produce a library with a titre of 5.56 X 10^9 pfu/ml.

text: Non-Embryogenic

text: Elaeis guineensis

text: EcoRI

text: XhoI

text: Callus

text: CNI (Oil Palm Non-Embryogenic Callus)

text: Phagemid


text: Malaysian Palm Oil Board

text: live

text: Generic

text: Total RNA was isolated from oil palm embryoids using the aqueous phenol extraction method. After total RNA extraction, poly (A)+ RNA was isolated using oligo-dT cellulose chromatography technique. Directional cDNA library were constructed using the ZAP-cDNA Synthesis Kit and the ZAP-cDNA Gigapack III Gold Cloning Kit (Stratagene). Oil palm embryoid cDNAs were cloned and amplified once to produce a library with a titre of 2.94 X 10^9 pfu/ml.

text: Proliferating Embryoid

text: Elaeis guineensis

text: EcoRI

text: XhoI

text: Embryoid

text: EA (Oil Palm Embryoid)

text: Phagemid


text: Malaysian Palm Oil Board

text: live

text: Generic

text: Total RNA was isolated from oil palm embryoids using the aqueous phenol extraction method. After total RNA extraction, poly (A)+ RNA was isolated using oligo-dT cellulose chromatography technique. Directional cDNA library were constructed using the ZAP-cDNA Synthesis Kit and the ZAP-cDNA Gigapack III Gold Cloning Kit (Stratagene). Oil palm embryoid cDNAs were cloned and amplified once to produce a library with a titre of 2.23 X 10^9 pfu/ml.

text: Proliferating Embryoid

text: Elaeis guineensis

text: EcoRI

text: XhoI

text: Embryoid

text: EO (Oil Palm Embryoid)

text: Phagemid


text: Malaysian Palm Oil Board

text: live

text: Generic

text: Total RNA was isolated from oil palm non-embryogenic callus using the aqueous phenol extraction method. After total RNA extraction, poly (A)+ RNA was isolated using oligo-dT cellulose chromatography technique. Directional cDNA library were constructed using the ZAP-cDNA Synthesis Kit and the ZAP-cDNA Gigapack III Gold Cloning Kit (Stratagene). Oil palm non-embryogenic callus cDNAs were cloned and amplified once to produce a library with a titre of 1.38 X 10^9 pfu/ml.

text: Non-Embryogenic

text: Elaeis guineensis

text: EcoRI

text: XhoI

text: Callus

text: CA (Oil Palm Non-Embryogenic Callus)

text: Plasmid


text: Malaysian Palm Oil Board

text: live

text: Generic

text: Total RNA was isolated from oil palm non-embryogenic callus using the aqueous phenol extraction method. After total RNA extraction, poly (A)+ RNA was isolated using oligo-dT cellulose chromatography technique. Directional cDNA library were constructed using the ZAP-cDNA Synthesis Kit and the ZAP-cDNA Gigapack III Gold Cloning Kit (Stratagene). Oil palm non-embryogenic callus cDNAs were cloned and amplified once to produce a library with a titre of 2.96 X 10^9 pfu/ml.

text: Non-Embryogenic

text: Elaeis guineensis

text: EcoRI

text: XhoI

text: Callus

text: CB (Oil Palm Non-Embryogenic Callus)

text: Plasmid


text: Malaysian Palm Oil Board

text: live

text: Generic

text: Total RNA was isolated from oil palm non-embryogenic callus using the aqueous phenol extraction method. After total RNA extraction, poly (A)+ RNA was isolated using oligo-dT cellulose chromatography technique. Directional cDNA library were constructed using the ZAP-cDNA Synthesis Kit and the ZAP-cDNA Gigapack III Gold Cloning Kit (Stratagene).

text: Non-Embryogenic

text: Elaeis guineensis

text: EcoRI

text: XhoI

text: Callus

text: CC (Oil Palm Non-Embryogenic Callus)

text: Plasmid


text: Malaysian Palm Oil Board

text: live

text: Generic

text: Total RNA was isolated from oil palm embryogenic callus using the aqueous phenol extraction method. After total RNA extraction, poly (A)+ RNA was isolated using oligo-dT cellulose chromatography technique. Directional cDNA library were constructed using the ZAP-cDNA Synthesis Kit and the ZAP-cDNA Gigapack III Gold Cloning Kit (Stratagene). Oil palm embryogenic callus cDNAs were cloned and amplified once to produce a library with a titre of 1.53 X 10^9 pfu/ml.

text: Embryogenic

text: Elaeis guineensis

text: EcoRI

text: XhoI

text: Callus

text: CEM (Oil Palm Embryogenic Callus)

text: Plasmid


text: Malaysian Palm Oil Board

text: live

text: Generic

text: Total RNA was isolated from oil palm non-embryogenic callus using the aqueous phenol extraction method. After total RNA extraction, poly (A)+ RNA was isolated using oligo-dT cellulose chromatography technique. Directional cDNA library were constructed using the ZAP-cDNA Synthesis Kit and the ZAP-cDNA Gigapack III Gold Cloning Kit (Stratagene). Oil palm non-embryogenic callus cDNAs were cloned and amplified once to produce a library with a titre of 1.22 X 10^9 pfu/ml.

text: Non-Embryogenic

text: Elaeis guineensis

text: EcoRI

text: XhoI

text: Callus

text: CNH (Oil Palm Non-Embryogenic Callus)

text: Plasmid