text: University of British Columbia

text: live

text: Generic

text: The suppressive subtractive hybridization cDNA library was constructed from mRNA of yeast like cells of Ophiostoma piceae.

text: Yeast like cells were grown under shaking condition for 5 days at 23oC

text: E.coli

characteristic: lab-host

text: Ophiostoma piceae

text: Yeast suppressive subtractive library

text: Plasmid

text: PCR 2.1-TOPO

characteristic: vector


status: PUBLIC

characteristic: External Id

text: University of British Columbia

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: The suppressive subtractive hybridization cDNA library was constructed from mRNA of mycelia of Ophiostoma piceae.

characteristic: description

text: Hyphae were grown on complete medium plate for 48 h at 23oC

tag: attribute

characteristic: dev-stage

text: E.coli

text: Ophiostoma piceae

characteristic: organism

text: Mycelial suppressive subtractive library

characteristic: title

text: Plasmid

text: PCR 2.1-TOPO


text: Cereal Research Centre, Agriculture and Agri-food Canada

text: live

text: Generic

text: AC McDuff

characteristic: cultivar

text: mRNA obtained from bolls 12 days after flowering

text: 12 days after flowering

text: Linum usitatissimum

text: NotI

text: MluI

text: Bolls

characteristic: tissue-type

text: pCMVSport6.1 (Invitrogen Corp.)


text: live

text: Generic

text: mRNA was prepared from antennae of late pupal and newly eclosed male Manduca sexta moths. The mRNA was reverse transcribed with an oligo-dT primer linked to a NotI site and unique tag (TCGGT). An adaptor with an EcoRI site and linker (CATTGTGTTGG) was ligated to the 5' end. The cDNA was cloned into the EcoRI and NotI sites of pGEM-11Zf(+). The W.M. Keck Center for Comparative and Functional Genomics at the University of Illinois at Urbana-Champaign prepared and randomly sequenced 400 clones from the library. The library was then subtracted with the most abundant clones and randomly sequenced.

text: newly eclosed adults and pharate adults

text: antennae

characteristic: organ

text: Manduca sexta

text: EcoRI

text: NotI

text: male

characteristic: sex

text: Manduca sexta male antennae pGEM-11Zf subtracted library

text: plasmid


text: Universita degli Studi d ella Basilicata

text: live

text: Generic

text: country=Netherlands

text: Bos taurus

text: placenta

text: Bos taurus placenta


text: The Institute of Genetics and Developmental Biology CAS

text: live

text: Generic

text: first day the flower opens

text: Gossypium hirsutum

text: ovule

text: Gossypium hirsutum ovule first day the flower opens


text: National Institute for Basic Biology

text: live

text: Generic

text: Oryzias latipes

text: HNI

characteristic: strain

text: Oryzias latipes HNI


text: Hiroshima University

text: live

text: Generic

text: blood cell

characteristic: cell type

text: adult

text: Ascidia sydneiensis samea

text: Ascidia sydneiensis samea whole blood cells cDNA


characteristic: External Id

text: Colorado State University

text: live

text: Generic

text: Four-day-old wheat seedlings were grown in the greenhouse and infested with Russian Wheat Aphid (RWA); the aphids were allowed to feed on the plants for ten days after which they were removed and leaf tissue was collected and frozen; tissues were also collected from seedlings that were not subjected to aphid feeding; Total RNA was extracted from the collected leaf tissue and subtraction experiment conducted with infested and un-infested leaf tissue; the library contains wheat transcripts enriched for response to RWA Feeding; single pass sequences

text: Leaves infested with Russian Wheat Aphid for ten days

text: Triticum aestivum

text: A. Wheat subtracted library enriched for Russian Wheat Aphid Feeding response


text: US Department of Agriculture, Agriculture Research Service, Pacific West Area, Western Regional Research Center

characteristic: INSDC center name

text: live

text: Generic

text: Chinese Spring

text: Plants were grown in a glasshouse. Anther meiotic stage was determined by removing anthers from individual primary florets. One anther was sacrificed for microscopic staging, and if determined to be between (and including) meiotic stages pre-meiosis and metaphase I, the remaining two anthers were collected and pooled for library construction. The tissue, total RNA, and poly(A) RNA were prepared, cDNA synthesised, and directionally ligated into pSPORT1 by Tim Sutton in the P Langridge Lab at the Department of Plant Science, University of Adelaide, Waite Campus, Australia. Average insert size 1.5Kb. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

text: Meiotic stages pre-meiosis-metaphase I

text: E. coli DH10B

characteristic: lab-host

text: Triticum aestivum

text: SalI

text: NotI

text: Anther

text: Wheat meiotic anther cDNA library

text: Plasmid

characteristic: vector


text: Children's Memorial Research Center

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: UI-R-FJ0 is a cDNA library containing the following tissue(s): rat embryo. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CATCTCTACT. This library was created for the University of Iowa Program for Rat Gene Discovery and Mapping (Val Sheffield, Bento Soares and Tom Casavant)

text: embryo

text: DH10B (Life Technologies) (T1 phage resistant)

text: Rattus norvegicus

text: EcoR I

text: Not I

text: Sprague-Dawley

text: embryo

text: Plasmid

text: pYX-Asc


text: National Cancer Institute / NIH

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: UI-R-FS0 is a cDNA library constructed from Swarm Rat Chondrosarcoma. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is AGCCGCCGAT. The tumor was provided by Dr Jeff Stevens from University of Iowa.

characteristic: description

text: ADULT

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid


text: Agriculture and Agri-Food Canada

text: live

text: Generic

text: SOLR

text: Solanum tuberosum

characteristic: organism

text: EcoRI

text: XhoI

text: Perimedulla (between vascular-ring and pith) of enlarging tubers

text: LIPTC

characteristic: title

text: pBluescript SK(-)


text: University of Illinois at Urbana-Champaign

text: live

text: Generic

text: testis

text: Sus scrofa

text: Eco RI

text: HindIII

text: male

text: Porcine testis phage library

text: Phage

text: T7 bacteriophage


text: Korea Research Institute of Bioscience and Biotechnology

text: live

text: Generic

text: Capsicum annuum


text: Mayo Foundation

text: live

text: Generic

text: T cell

text: 11-12 weeks old

characteristic: dev-stage

text: Mus musculus

text: male

text: prostate

characteristic: tissue-type

text: C57BL/6 mice Prostate T lymphocyte Leader


text: Institute of Biology - University of Brasilia

text: live

text: Generic

text: Pb Lambda Zap Express Library

text: Paracoccidioides brasiliensis

text: Mycelium and yeast cells from Paracoccidioides brasiliensis


text: KTH

text: live

text: Generic

text: CDNA was prepared from whole aorta divided in atherosclerotic plaque prone regions (aortic arch and abdominal aorta proximal part) and less plaque prone regions (descending thoracic aorta and abdominal aorta distal part). CDNA was fragmented with DpnII, linker ligated and amplified to generated starting material for representational difference analysis (RDA). The two cDNA pools were subjected to iterative RDA subtraction and amplification to enrich for gene fragments differentially expressed at early stages of atherosclerosis.

text: 8 weeks old

text: aorta

characteristic: organ

text: Mus musculus

text: DpnII

text: DpnII

text: male

characteristic: sex

text: ApoE-/- and LDLR-/- on C57BL/6x 129 background

text: cDNA from mouse aorta


text: University of California, Riverside

text: live

text: Generic

text: 'Washington' navel orange

characteristic: cultivar

text: Differentially expressed cDNAs from leaf axil buds of 'Washington' navel orange under low temperature field conditions in Riverside CA.

text: 18-year-old trees - low-temperature treated

text: Citrus sinensis

text: leaf axil bud

text: 'Washington' navel orange leaf axil bud

text: plasmid

text: pGEM-Teasy


characteristic: External Id

text: Max Planck Institute for Chemical Ecology

text: live

text: Generic

text: DDRT-PCR (Nucleic Acid Research 21, 4272-4280, 1993)

text: rosette

text: E.coli

text: Nicotiana attenuata

text: EcoRI

text: EcoRI

text: shoot

text: cDNAs of Nicotiana attenuatta insect-responsive genes isolated by mRNA differential-display RT-PCR

text: plasmid

text: pCR 2.1-TOPO


characteristic: INSDC center name

text: live

text: Generic

text: Subtractive hybridization based on magnetic beads (DYNAL), cDNA cloning according to SMART cDNA Library Construction Kit (Clontech)

text: rosette

text: E.coli

text: Nicotiana attenuata

text: BamHI

text: KpnI

text: shoot

text: cDNAs of Nicotiana attenuatta insect-responsive genes isolated by subtractive hybrydization using magnetic beads

text: plasmid


text: live

text: Generic

text: cDNA-AFLP display

text: rosette

text: E.coli

characteristic: lab-host

text: Nicotiana attenuata

text: EcoRI

text: EcoRI

text: shoot

text: cDNAs of Manduca sexta regurgitant induced Nicotiana attenuata genes isolated by cDNA-AFLP display

text: plasmid

text: pCR 2.1-TOPO


text: Nanjing Normal University

text: live

text: Generic

text: These sequences were differentially expressed genes between stage two and stage three ovary of Eriocheir japonica sinensis which were obtained by SSH and identified by cDNA macroarray.

text: stage two and stage three

text: ovary

text: Eriocheir sinensis

text: female

text: Subtracted cDNA library of Eriocheir japonica sinensis ovary between stage two stage three


text: E. I. DuPont de Nemours and Company

text: live

text: Generic

text: barley sheath, developing seedling

text: Hordeum vulgare

text: EcoRI

text: XhoI

text: seedling

text: pBluescript SK+

characteristic: vector


text: E. I. DuPont de Nemours and Company

characteristic: INSDC first public

text: live

text: Generic

text: Wheat (Triticum aestivum) pre-meiotic anthers JIC

text: Triticum aestivum

text: EcoRI

text: XhoI

text: anthers

text: pBluescript SK+


text: E. I. DuPont de Nemours and Company

characteristic: INSDC last update

text: live

text: Generic

text: Wheat (Triticum aestivum) yellow rust infested tissue JIC

text: Triticum aestivum

text: EcoRI

text: XhoI

text: pBluescript SK+


text: E. I. DuPont de Nemours and Company

text: live

characteristic: INSDC status

text: Generic

text: Chinese Spring

text: To examine gene expression patterns in wheat anthers undergoing meiosis. Library was made at the Waite Institute in Australia.

text: Triticum aestivum

text: NotI

text: SalI

text: anthers


text: E. I. DuPont de Nemours and Company

text: live

text: Generic

characteristic: NCBI submission model

text: Spring wheat

text: Wheat (Triticum aestivum L.) leaf 7 day old seedling, light grown

text: Triticum aestivum

text: EcoRI

text: XhoI

text: leaf

text: pBluescript SK+


text: E. I. DuPont de Nemours and Company

text: live

text: Generic

characteristic: NCBI submission package

text: Wheat (Triticum aestivum L.) root; 7 day old seedling, light grown

text: Triticum aestivum

text: EcoRI

text: XhoI

text: root

text: pBluescript SK+


text: E. I. DuPont de Nemours and Company

text: live

text: Generic

text: Wheat (Triticum aestivum L.) leaf 7 day old seedling, light grown (normalized)

characteristic: description

text: Triticum aestivum

text: EcoRI

text: XhoI

text: leaf

text: pBluescript SK+


text: E. I. DuPont de Nemours and Company

text: live

text: Generic

text: Wheat (Triticum aestivum L.) developing root and leaf

text: Triticum aestivum

characteristic: organism

text: EcoRI

text: XhoI

text: root and leaf

text: pBluescript SK+


text: E. I. DuPont de Nemours and Company

text: live

text: Generic

text: Wheat (Triticum aestivum L.) leaf 7 day old etiolated seedling

text: Triticum aestivum

text: EcoRI

text: XhoI

text: leaf

characteristic: title

text: pBluescript SK+


text: E. I. DuPont de Nemours and Company

text: live

text: Generic

text: Wheat (Triticum aestivum L.) leaf 7 day old etiolated seedling (normalized)

text: Triticum aestivum

text: EcoRI

text: XhoI

text: leaf

text: pBluescript SK+


text: E. I. DuPont de Nemours and Company

text: live

text: Generic

text: Wheat (Triticum aestivum L.) root; 7 day old etiolated seedling

text: Triticum aestivum

text: EcoRI

text: XhoI

text: root

text: pBluescript SK+


text: E. I. DuPont de Nemours and Company

text: live

text: Generic

text: Wheat (Triticum aestivum L.) root; normalized from wre1 library

text: Triticum aestivum

text: EcoRI

text: XhoI

text: root

text: pBluescript SK+


text: E. I. DuPont de Nemours and Company

text: live

text: Generic

text: Stephens

text: Wheat (Triticum aestivum L.) seedlings 0 hr after inoculation with Erysiphe graminis f. sp tritici

text: Triticum aestivum

text: EcoRI

text: XhoI

text: leaf

text: pBluescript SK+


text: E. I. DuPont de Nemours and Company

text: live

text: Generic

text: Stephens

text: Wheat (Triticum aestivum L.) seedlings 1 hr after inoculation with Erysiphe graminis f. sp tritici

text: Triticum aestivum

text: EcoRI

text: XhoI

text: leaf

text: pBluescript SK+


text: E. I. DuPont de Nemours and Company

text: live

text: Generic

text: Stephens

text: Wheat (Triticum aestivum L.) seedlings 1 hr after inoculation with Erysiphe graminis f. sp tritici and treatment with 6-iodo-3-propyl-2-propyloxy-4(3H)-quinazolinone

text: Triticum aestivum

text: EcoRI

text: XhoI

text: leaf

text: Lambda Zap


text: E. I. DuPont de Nemours and Company

text: live

text: Generic

text: Stephens

text: Wheat (Triticum aestivum L.) seedlings 1 hr after treatment with 6-iodo-3-propyl-2-propyloxy-4(3H)-quinazolinone

text: Triticum aestivum

text: EcoRI

text: XhoI

text: leaf

text: pBluescript SK+


text: E. I. DuPont de Nemours and Company

text: live

text: Generic

text: Stephens

text: Wheat (Triticum aestivum L.) WLMK8 cDNAs subtracted with WLM0 cDNAs

text: Triticum aestivum

text: leaf

text: PGEM-T Easy


text: E. I. DuPont de Nemours and Company

text: live

text: Generic

text: Stephens

text: Wheat (Triticum aestivum L.) wlk8 cDNAs substracted with WLM0 cDNAs

text: Triticum aestivum

text: EcoRI

text: XhoI

text: leaf

text: PGEM-T Easy


text: E. I. DuPont de Nemours and Company

text: live

text: Generic

text: Stephens

text: Wheat (Triticum aestivum L.) wr1 cDNAs subtracted with wl1 cDNAs

text: Triticum aestivum

text: root


text: E. I. DuPont de Nemours and Company

text: live

text: Generic

text: Stephens

text: Wheat (Triticum aestivum L.) seedlings 12 hr after inoculation w/ E. graminis

text: Triticum aestivum

text: EcoRI

text: XhoI

text: leaf

text: pBluescript SK+


text: E. I. DuPont de Nemours and Company

text: live

text: Generic

text: Stephens

text: Wheat (Triticum aestivum L.) seedlings 4 hr after inoculation w/E. graminis

text: Triticum aestivum

text: EcoRI

text: XhoI

text: leaf

text: pBluescript SK+


text: E. I. DuPont de Nemours and Company

text: live

text: Generic

text: Stephens

text: Wheat (Triticum aestivum L.) seedlings 24 hr after inoculation w/E. graminis

text: Triticum aestivum

text: EcoRI

text: XhoI

text: leaf

text: pBluescript SK+


text: E. I. DuPont de Nemours and Company

text: live

text: Generic

text: Stephens

text: Wheat (Triticum aestivum L.) seedlings 96 hr after inoculation w/ E. graminis

text: Triticum aestivum

text: EcoRI

text: XhoI

text: leaf

text: pBluescript SK+


text: E. I. DuPont de Nemours and Company

text: live

text: Generic

text: Stephens

text: Wheat (Triticum aestivum L.) seedlings 4 hr after inoculation w/ E. graminis and 6-iodo-3-propyl-2-propyloxy-4(3H)-quinazolinone

text: Triticum aestivum

text: EcoRI

text: XhoI

text: leaf

text: pBluescript SK+


text: E. I. DuPont de Nemours and Company

text: live

text: Generic

text: Stephens

text: Wheat (Triticum aestivum L.) seedlings 8 hr after inoculation w/ E. graminis and 6-iodo-3-propyl-2-propyloxy-4(3H)-quinazolinone

text: Triticum aestivum

text: EcoRI

text: XhoI

text: leaf

text: pBluescript SK+


text: E. I. DuPont de Nemours and Company

text: live

text: Generic

text: Stephens

text: Wheat (Triticum aestivum L.) seedlings 4 hr after treatment with 6-iodo-3-propyl-2-propyloxy-4(3H)-quinazolinone

text: Triticum aestivum

text: EcoRI

text: XhoI

text: leaf

text: pBluescript SK+


text: E. I. DuPont de Nemours and Company

text: live

text: Generic

text: Stephens

text: Wheat (Triticum aestivum L.) seedlings 8 hr after treatment with 6-iodo-3-propyl-2-propyloxy-4(3H)-quinazolinone

text: Triticum aestivum

text: EcoRI

text: XhoI

text: leaf


text: E. I. DuPont de Nemours and Company

text: live

text: Generic

text: hard red spring

text: Wheat (Triticum aestivum L.) kernel malted 55 hours at 22 C

text: Triticum aestivum

text: EcoRI

text: XhoI

text: kernel


text: E. I. DuPont de Nemours and Company

text: live

text: Generic

text: hard red spring

text: Wheat (Triticum aestivum L.) kernel malted 175 hours at 4 C

text: Triticum aestivum

text: EcoRI

text: XhoI

text: kernel


text: E. I. DuPont de Nemours and Company

text: live

text: Generic

text: Wheat (Triticum aestivum L.) developing kernel, 3 days after anthesis.

text: Triticum aestivum

text: kernel

text: pBluescript SK+


text: E. I. DuPont de Nemours and Company

text: live

text: Generic

text: Wheat (Triticum aestivum L.) developing kernel, 7 days after anthesis.

text: Triticum aestivum

text: EcoRI

text: XhoI

text: kernel

text: pBluescript SK+


text: E. I. DuPont de Nemours and Company

text: live

text: Generic

text: Wheat (Triticum aestivum L.) developing kernel, 14 days after anthesis.

text: Triticum aestivum

text: EcoRI

text: XhoI

text: kernel

text: pBluescript SK+


text: E. I. DuPont de Nemours and Company

text: live

text: Generic

text: Spring wheat

text: Wheat (Triticum aestivum L.) developing kernel, 21 days after anthesis.

text: Triticum aestivum

text: EcoRI

text: XhoI

text: kernel

text: pBluescript SK+


text: E. I. DuPont de Nemours and Company

text: live

text: Generic

text: Spring wheat

text: Wheat (Triticum aestivum L.) developing kernel, 30 days after anthesis.

text: Triticum aestivum

text: EcoRI

text: XhoI

text: kernel

text: pBluescript SK+


text: E. I. DuPont de Nemours and Company

text: live

text: Generic

text: Spring wheat

text: Wheat (Triticum aestivum L.) kernel malted 175 hours at 4 C, normalized

text: Triticum aestivum

text: EcoRI

text: XhoI

text: kernel

text: pBluescript SK+


text: E. I. DuPont de Nemours and Company

text: live

text: Generic

text: Spring wheat

text: Wheat (Triticum aestivu, Spring Wheat) kernels 3, 7, 14 and 21 days after anthesis.

text: Triticum aestivum

text: EcoRI

text: XhoI

text: kernel

text: pBluescript SK+


text: E. I. DuPont de Nemours and Company

text: live

text: Generic

text: Spring wheat

text: Wheat (Triticum aestivum L., HiLine) developing kernels 3, 7, 14 and 21 days after anthesis normalized.

text: Triticum aestivum

text: EcoRI

text: XhoI

text: kernel

text: pBluescript SK+


text: E. I. DuPont de Nemours and Company

text: live

text: Generic

text: Wheat (Triticum aestivum) developing root (full length)

text: Triticum aestivum

text: EcoRI

text: XhoI

text: root

text: pBluescript SK+


text: E. I. DuPont de Nemours and Company

text: live

text: Generic

text: Wheat developing seedling full length

text: Triticum aestivum

text: EcoRI

text: XhoI

text: seedling

text: pBluescript SK+


text: E. I. DuPont de Nemours and Company

text: live

text: Generic

text: Wheat 10 day old seedling full length without biotin

text: Triticum aestivum

text: EcoRI

text: XhoI

text: seedling

text: pBluescript SK+


text: E. I. DuPont de Nemours and Company

text: live

text: Generic

text: Wheat (Triticum aestivum) test immature infloresence treated with DuPont Hybrinova CHA and driver and immature infloresence treated with solvent alone

text: Triticum aestivum

text: SmaI

text: inflorescence

text: PGEM-T Easy


text: E. I. DuPont de Nemours and Company

text: live

text: Generic

text: Wheat (Triticum aestivum, Hi Line) developing endosperm 2-7 DPA

text: Triticum aestivum

text: EcoRI

text: XhoI

text: endosperm

text: pBluescript SK+


text: E. I. DuPont de Nemours and Company

text: live

text: Generic

text: Wheat (Triticum aestivum, Hi Line) developing inflorescence +/- 4 cm

text: Triticum aestivum

text: EcoRI

text: XhoI

text: inflorescence

text: pBluescript SK+


text: E. I. DuPont de Nemours and Company

text: live

text: Generic

text: Wheat (Triticum aestivum, Hi Line) immature pistils

text: Triticum aestivum

text: EcoRI

text: XhoI

text: pistils

text: pBluescript SK+


text: E. I. DuPont de Nemours and Company

text: live

text: Generic

text: Wheat (Triticum aestivum, Hi Line) lemma and palea

text: Triticum aestivum

text: EcoRI

text: XhoI

text: lemma and palea

text: pBluescript SK+


text: E. I. DuPont de Nemours and Company

text: live

text: Generic

text: Wheat (Triticum aestivum, Hi Line) developing endosperm 8-15 DPA

text: Triticum aestivum

text: EcoRI

text: XhoI

text: endosperm

text: pBluescript SK+


text: E. I. DuPont de Nemours and Company

text: live

text: Generic

text: Wheat, Polk cultivar (resistant), infected with septoria tritici strain A 24 hours after infection

text: Triticum aestivum

text: SmaI

text: leaf

text: PGEM-T Easy


text: E. I. DuPont de Nemours and Company

text: live

text: Generic

text: Wheat, Polk cultivar (resistant), infected with Septoria tritici strain A 48 hours after infection

text: Triticum aestivum

text: SmaI

text: leaf

text: PGEM-T Easy


text: E. I. DuPont de Nemours and Company

text: live

text: Generic

text: Wheat (Triticum aestivum) test immature infloresence treated with DuPont Hybrinova CHA and driver and immature infloresence treated with solvent alone directionally cloned

text: Triticum aestivum

text: BamHI

text: inflorescence

text: PGEM-T Easy


text: E. I. DuPont de Nemours and Company

text: live

text: Generic

text: Wheat (Triticum aestivum, Hi Line) embryos 14 DAP

text: Triticum aestivum

text: EcoRI

text: XhoI

text: embryo

text: pBluescript SK+


text: E. I. DuPont de Nemours and Company

text: live

text: Generic

text: Wheat (Triticum aestivum, Hi Line) immature anthers

text: Triticum aestivum

text: EcoRI

text: XhoI

text: anthers

text: pBluescript SK+


text: E. I. DuPont de Nemours and Company

text: live

text: Generic

text: Wheat (Triticum aestivum) immature infloresence treated with DuPont Hybrinova CHA

text: Triticum aestivum

text: EcoRI

text: XhoI

text: inflorescence

text: pBluescript SK+


text: E. I. DuPont de Nemours and Company

text: live

text: Generic

text: Wheat (Triticum aestivum, Hi Line) flag leaf

text: Triticum aestivum

text: EcoRI

text: XhoI

text: leaf

text: pBluescript SK+


text: E. I. DuPont de Nemours and Company

text: live

text: Generic

text: Riband (susceptible) wheat leaves infected with Septoria tritici strain A, 24 hours after infection, subtracted w/ comparable uninfected leaves

text: Triticum aestivum

text: SmaI

text: leaf

text: PGEM-T Easy


text: E. I. DuPont de Nemours and Company

text: live

text: Generic

text: Riband (susceptible) wheat leaves infected with Septoria tritici strain A, 48 hours after infection, subtracted w/ comparable uninfected leaves

text: Triticum aestivum

text: SmaI

text: leaf

text: PGEM-T Easy


text: Nanjing Medical University

text: live

text: Generic

text: These sequences obtained by SSH and identified by cDNA microarray were differentially expressed between hyperlipidemia-sensitive and -insensitive livers of New Zealand White Rabbit.

text: adult

characteristic: dev-stage

text: liver

text: Oryctolagus cuniculus

text: male

text: New Zealand White

characteristic: strain

text: Subtracted cDNA library of livers between hyperlipidemia-sensitive and -insensitive New Zealand White Rabbit


text: North Carolina State University

text: live

text: Generic

text: Log phase of aflatoxin production

text: Aspergillus flavus

text: Aspergillus flavus Lambda Zap cDNA Library

text: Plasmid

text: pBluescript


text: Institute of Genetics, Fudan University

text: live

text: Generic

text: Wuyunjing No. 7

text: suppressed subtract hybridizated cDNA prepared from rice callus mRNAs ligating to pGEM-T

text: Oryza sativa

text: Rice callus and differential tissue SSH library

text: pGEM-T


text: Instituto de Agroindustria, Universidad de La Frontera

text: live

text: Generic

text: Differential Display fragment isolated from leaves of cold acclimated plants and cloned in pGEM-T-Easy (Promega)

text: Deschampsia antarctica

text: EcoRI

text: EcoRI

text: Leaf

characteristic: tissue-type

text: Differential display fragment

text: plasmid

text: pGEM-T-Easy


text: University of Adelaide

text: live

text: Generic

text: 75-1-127 LINE

text: 12 hours after inoculation with Magnaporthe grisea Philippine isolate PO6-6

text: Oryza sativa

text: SalI

text: NotI

text: leaves

text: Magnaporthe grisea infected rice cDNA, 12 hours


text: Institute of Biochemistry, University of Goettingen

text: live

text: Generic

text: Stage 28-30

text: Xenopus laevis

text: Embryonic liver diverticulum

text: Xenopus embryonic liver diverticulum plasmid library


text: live

text: Generic

text: liver

characteristic: organ

text: Xenopus laevis

text: Xhol

text: Female

characteristic: sex

text: Adult liver

text: Xenopus adult liver ZAP Express phage library

text: pBK-CMV


characteristic: External Id

text: Instituto de Agroquimica y Tecnologia de Alimentos (IATA-CSIC)

text: live

text: Generic

text: from decayed fruit

characteristic: isolate

text: Malus x domestica

text: Penicillium expansum

text: Penicillium expansum from apple fruit


text: University of Le Havre

characteristic: INSDC center name

text: live

text: Generic

text: Dreissena polymorpha

text: Dreissena polymorpha mRNA


text: University of Illinois

text: live

text: Generic

characteristic: cultivar

characteristic: lab-host

text: Oryza sativa

text: Root

text: Uni-ZAP XR

characteristic: vector


text: National Institutes of Health,

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Ixodes scapularis

text: Ixodes scapularis SMART cDNA


text: Michigan State University

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Library made from pooled tissue from adipose, adrenal gland, blood leukocytes, brain, cartilage, eye, heart, intestine, kidney, liver, lung lymph nodes, mammary gland, myogenic satellite cells, ovary, pancreas, pituitary gland, placenta, skin, spinal cord, spleen, stomach, tendon, testes, uterus, and vascular from various developmental and physiological stages.

characteristic: description

text: pooled

text: pooled

text: Sus scrofa

text: NotI

text: SalI

text: Male and female

text: pooled

text: MPL

text: plasmid


text: RIKEN

text: live

text: Generic

text: Equus caballus

characteristic: organism

text: Equus caballus embryonic stem-like cells

characteristic: title


text: INRA

text: live

text: Generic

text: Compression wood was induced in four 14-year old maritime pine clones by artifitial bending. Control clone (no bending) were sampled for xylem associated with early wood and late wood at the beginning (April) and at the end (August) of the growing seasons. Differentiating xylem corresponding to Early wood, late wood, compression wood and opposite wood were used for mRNA fingerprinting using the cDNA-AFLPP assay.

text: Pinus pinaster

text: xylem

characteristic: tissue-type

text: Pinus pinaster xylem


text: live

text: Generic

text: Golden Delicious

text: cDNA obtained by differential display RT-PCR from apple fruit infected with Penicillium expansum

text: Malus domestica

text: fruit

text: Malus x domestica fruit infected with Penicillium expansum


text: Kyoto University

text: live

text: Generic

text: Molgula tectiformis

text: Molgula tectiformis library


text: New Mexico State University

text: live

text: Generic

text: immature fruit

characteristic: dev-stage

text: placenta

characteristic: organ

text: Capsicum chinense

text: habanero placental cDNA

text: Phage

text: Lambda Zap II


characteristic: External Id

text: University of Delaware

text: live

text: Generic

text: E.coli EMDH10B

text: Gallus gallus

text: Male and Female

characteristic: sex

text: thymus, bursa, spleen, PBL, bone marrow

text: Chicken lymphoid cDNA library (pgn1c)

text: pCMVSPORT 6


text: USDA-ARS-Plant Sciences Institute

characteristic: INSDC center name

text: live

text: Generic

text: Wildtype

text: Isolated from differential display of cDNAs from leaves of spotted knapweed (Centaurea maculosa Lam.) after treatment with the fungal protein Nep1.

text: Rosette

text: GH Competent Cells, E. coli

text: Centaurea maculosa

text: leaf

text: Centaurea maculosa Differential Display

text: Plasmid

text: PCR-TRAP Vector


text: USDA-ARS-Plant Sciences Institute

text: live

text: Generic

text: Field collection

characteristic: cultivar

text: Isolated from differential display of cDNAs from leaves of dandelion (Taraxacum officinale) after treatment with the fungal protein Nep1.

text: Rosette

text: GH Competent Cells, E. coli

text: Taraxacum officinale

text: leaf

text: Taraxacum officinale Differential Display

text: Plasmid

text: PCR-TRAP Vector


text: USDA-ARS-Plant Sciences Institute

text: live

text: Generic

text: Wildtype

text: Isolated from differential display of cDNAs from leaves of Arabidopsis thaliana wildtype after treatment with the fungal protein Nep1.

text: Rosette

text: GH Competent Cells, E. coli

characteristic: lab-host

text: Arabidopsis thaliana

text: leaf

text: Arabidopsis thaliana Differential Display

text: Plasmid

text: PCR-TRAP Vector


text: Masterfoods

text: live

text: Generic

text: Whole organ

characteristic: cell type

text: Bean and leaf tissue from an Amelonado type Cacao tree.

text: maturity

text: XL-1 Blue MRF'

text: Theobroma cacao

text: Amelonado type

characteristic: strain

text: Mature leaf and mature bean

text: Cac_BL (Bean and Leaf from Amelonardo type Cacao)

text: pBK-CMV

characteristic: vector


text: The Institute of Physical and Chemical Research (RIKEN)

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: pooled tissues: (tissue_type=bladder,sex=mix,cell_line=RCB-0544 MBT-2),(tissue_type=colon,sex=mix,cell_line=RCB-0549 Cle-H3),(sex=mix,cell_line=RCB-0559 K-1 . F1),(sex=mix,cell_line=RCB-0035 WEHI-3),(sex=mix,cell_line=RCB-1283 B16 melanoma),(sex=mix,cell_line=RCB-0464 Meth-A),(sex=mix,cell_line=RCB-0545 OHTA),(sex=mix,cell_type=nullipotent stem cell,cell_line=CRL-2070 NE),(tissue_type=kidney,sex=mix,cell_line=CCL-142 RAG),(tissue_type=submandibular gland,sex=mix,cell_line=CRL-1734 SCA-9 clone 15),(sex=mix,cell_line=CRL-2116 JC),(sex=mix,cell_line=CRL-1751 WEHI 164),(tissue_type=brain,sex=mix,cell_line=CRL-1443 BC3H1),(sex=mix,cell_type=B cells,cell_line=CRL-1669 BCL1 Clone 13.20-3B3),(tissue_type=bone marrow,sex=mix,cell_type=stroma cell,cell_line=CRL-2028 SR-4987),(sex=mix,cell_type=Leydig cells,cell_line=CRL-2065 MLTC-1),(sex=mix,cell_type=B cells,cell_line=CRL-1702 WEHI 231)

text: Mus musculus

text: RIKEN full-length enriched, pooled tissues, bladder, etc.


text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: adult

text: Mus musculus

text: male

text: liver and lung

text: RIKEN full-length enriched, adult male liver and lung


text: China Medical University

text: live

text: Generic

text: cancer cells

text: adult

text: Homo sapiens

characteristic: organism

text: male

text: stomach

text: subtracted cDNA Library (Stomach Cancer as Tester, SCT)

characteristic: title


text: live

text: Generic

text: 5 days embryo

characteristic: dev-stage

text: Mus musculus

text: whole body

characteristic: tissue-type

text: RIKEN full-length enriched, 5 days embryo whole body


characteristic: External Id

text: live

text: Generic

text: 14 days pregnant adult

text: Mus musculus

text: female

text: amnion

text: RIKEN full-length enriched, 14 days pregnant adult female amnion


text: live

text: Generic

text: pooled tissues: (dev_stage=1 cell embryo,sex=mix,cell_type=1 cell),(dev_stage=3 cells embryo,sex=mix,cell_type=3 cells),(dev_stage=4 cells embryo,sex=mix,cell_type=4 cells),(dev_stage=morula,tissue_type=whole body,sex=mix,cell_type=morula),(dev_stage=4 days embryo,tissue_type=whole body,sex=mix),(dev_stage=5 days embryo,tissue_type=whole body,sex=mix)

characteristic: description

text: Mus musculus

text: RIKEN full-length enriched, pooled tissues, 1 cell embryo, etc.


characteristic: INSDC center name

text: live

text: Generic

text: pooled tissues: (dev_stage=14 days embryo,tissue_type=liver,sex=mix),(dev_stage=11 days pregnant, adult,tissue_type=placenta,sex=female), (dev_stage=12 days pregnant, adult,tissue_type=placenta,sex=female),(dev_stage=13 days pregnant, adult,tissue_type=placenta,sex=female), (dev_stage=14 days pregnant, adult,tissue_type=placenta,sex=female),(dev_stage=15 days pregnant, adult,tissue_type=placenta,sex=female), (dev_stage=14 days pregnant, adult,tissue_type=amnion,sex=female)

text: Mus musculus

text: RIKEN full-length enriched, pooled tissues, 14 days embryo, etc.


text: live

text: Generic

text: pooled tissues: (sex=mix,cell_line=TIB-55BB88), (sex=mix,cell_line=CRL-1722 L5178Y-R)

text: Mus musculus

text: RIKEN full-length enriched, pooled tissues, cell_line=TIB-55BB88, etc.


text: live

text: Generic

text: 13 days pregnant adult

text: Mus musculus

text: female

characteristic: sex

text: placenta

text: RIKEN full-length enriched, 13 days pregnant adult female placenta


text: live

text: Generic

text: 14 days pregnant adult

text: Mus musculus

text: female

text: placenta

text: RIKEN full-length enriched, 14 days pregnant adult female placenta


text: live

text: Generic

text: 15 days pregnant adult

text: Mus musculus

text: female

text: placenta

text: RIKEN full-length enriched, 15 days pregnant adult female placenta


text: live

text: Generic

text: 1 cell

text: 1 cell embryo

text: Mus musculus

text: RIKEN full-length enriched, 1 cell embryo


text: The Institute of Physical and Chemical Research (RIKEN)

text: live

text: Generic

text: 3 cells

text: 3 cells embryo

text: Mus musculus

text: RIKEN full-length enriched, 3 cells embryo


text: live

text: Generic

text: 4 cells

text: 4 cells embryo

text: Mus musculus

text: RIKEN full-length enriched, 4 cells embryo


text: live

text: Generic

text: blastocyst

text: Mus musculus

text: RIKEN full-length enriched, blastocyst


text: live

text: Generic

text: 4 days embryo

text: Mus musculus

text: whole body

text: RIKEN full-length enriched, 4 days embryo whole body


text: live

text: Generic

text: 16 days embryo

text: Mus musculus

text: heart

text: RIKEN full-length enriched, 16 days embryo heart


text: live

text: Generic

text: 17 days embryo

text: Mus musculus

text: heart

text: RIKEN full-length enriched, 17 days embryo heart


text: live

text: Generic

text: 16 days embryo

text: Mus musculus

text: kidney

text: RIKEN full-length enriched, 16 days embryo kidney


text: live

text: Generic

text: 17 days embryo

text: Mus musculus

text: kidney

text: RIKEN full-length enriched, 17 days embryo kidney


text: live

text: Generic

text: 17 days embryo

text: Mus musculus

text: stomach

text: RIKEN full-length enriched, 17 days embryo stomach


text: live

text: Generic

text: 17 days pregnant adult

text: Mus musculus

text: female

text: amnion

text: RIKEN full-length enriched, 17 days pregnant adult female amnion


text: live

text: Generic

text: CRL-1722 L5178Y-R

characteristic: cell-line

text: Mus musculus

text: RIKEN full-length enriched, CRL-1722 L5178Y-R


text: live

text: Generic

text: TIB-55 BB88

text: Mus musculus

text: BALB/C

text: RIKEN full-length enriched, TIB-55 BB88


text: live

text: Generic

text: 10 days neonate

text: Mus musculus

text: brain

text: RIKEN full-length enriched, 10 days neonate brain


text: live

text: Generic

text: 10 days neonate

text: Mus musculus

text: heart

text: RIKEN full-length enriched, 10 days neonate heart


text: live

text: Generic

text: 13 days embryo

text: Mus musculus

text: whole body

text: RIKEN full-length enriched, 13 days embryo whole body


text: live

text: Generic

text: 14 days embryo

text: Mus musculus

text: whole body

text: RIKEN full-length enriched, 14 days embryo whole body


text: live

text: Generic

text: 16 days embryo

text: Mus musculus

text: whole body

text: RIKEN full-length enriched, 16 days embryo whole body


text: live

text: Generic

text: 17 days embryo

text: Mus musculus

text: whole body

text: RIKEN full-length enriched, 17 days embryo whole body


text: The Institute of Physical and Chemical Research (RIKEN)

text: live

text: Generic

text: 15 days pregnant adult

text: Mus musculus

text: female

text: amnion

text: RIKEN full-length enriched, 15 days pregnant adult female amnion


text: live

text: Generic

text: pooled tissues: (dev_stage=16 days embryo,tissue_type=heart,sex=mix),(dev_stage=16 days embryo,tissue_type=kidney,sex=mix),(dev_stage=17 days embryo,tissue_type=heart,sex=mix),(dev_stage=17 days embryo,tissue_type=stomach,sex=mix),(dev_stage=17 days embryo,tissue_type=kidney,sex=mix),(dev_stage=17 days pregnant, adult,tissue_type=amnion,sex=female), (dev_stage=13 days embryo,tissue_type=liver,sex=mix)

text: Mus musculus

text: RIKEN full-length enriched, pooled tissues, 16 days embryo, etc.


text: live

text: Generic

text: (dev_stage=adult,tissue_type=spleen,sex=male), (dev_stage=adult,tissue_type=kidney,sex=male), (dev_stage=adult,tissue_type=testis,sex=male), (dev_stage=adult,tissue_type=thymus,sex=male), (dev_stage=adult,tissue_type=heart,sex=male), (dev_stage=adult,tissue_type=colon,sex=male), (dev_stage=adult,tissue_type=stomach,sex=male), (dev_stage=adult,tissue_type=liver,sex=male), (dev_stage=13 days embryo,tissue_type=whole body,sex=mix),(dev_stage=14 days embryo,tissue_type=whole body,sex=mix),(dev_stage=16 days embryo,tissue_type=whole body,sex=mix),(dev_stage=17 days embryo,tissue_type=whole body,sex=mix),(dev_stage=15 days pregnant, adult,tissue_type=amnion,sex=female),(dev_stage=10 days neonate,tissue_type=brain,sex=mix),(dev_stage=10 days neonate,tissue_type=thymus,sex=mix),(dev_stage=10 days neonate,tissue_type=heart,sex=mix)

text: Mus musculus

text: RIKEN full-length enriched, pooled tissues, adult spleen, etc.


text: live

text: Generic

text: 15 days embryo

text: Mus musculus

text: whole body

text: RIKEN full-length enriched, 15 days embryo whole body


text: live

text: Generic

text: 18 days embryo

text: Mus musculus

text: whole body

text: RIKEN full-length enriched, 18 days embryo whole body


text: live

text: Generic

text: 17.5 days embryo

text: Mus musculus

text: whole body

text: RIKEN full-length enriched, 17.5 days embryo whole body


text: live

text: Generic

text: macrophage

characteristic: cell type

text: Mus musculus

text: bone marrow

text: RIKEN full-length enriched, bone marrow macrophage


text: live

text: Generic

text: NOD-derived CD11c +ve dendritic cells

text: Mus musculus

text: NOD

characteristic: strain

text: RIKEN full-length enriched, NOD-derived CD11c +ve dendritic cells


text: live

text: Generic

text: thymic cells

text: Mus musculus

text: NOD

text: thymus

text: RIKEN full-length enriched, 2 days neonate thymus thymic cells (NOD)


text: British Columbia Cancer Agency

text: live

text: Generic

text: mRNA isolated from 500 pairs of hand-dissected salivary glands from mixed stage animals was used to construct an oligo-dT-primed directional cDNA library

text: 16, 18, 20, 22, and 24 hrs after puparium formation at 18oC

characteristic: lab-host

text: Drosophila melanogaster

text: NotI

text: SalI

text: male and female

text: Oregon R

text: salivary gland

text: Plasmid

characteristic: vector


text: Massachusetts General Hospital

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: ES

text: undifferentiated

text: Mus musculus

text: male

text: mouse J1 male ES cell cDNA


text: UC Davis Department of Plant Pathology

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Cabernet Sauvignon

characteristic: cultivar

text: CA12LI is a cDNA library of Cabernet Sauvignon leaves. The leaves were collected on September 20, 2001, in Napa Valley, California, and represent leaves in late season development. These leaves were symptomatic and verified to be infected with the bacterial pathogen, Xylella fastidiosa, based on a diagnostic assay using PCR and Xylella-specific primer pairs. cDNAs were made by oligo-dT priming and directionally cloned. 5'and 3' adaptors were used in cloning as follows: 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' and 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)NN-3'. Library was constructed using the Clontech Creator SMART kit and size-selected to contain the 0.5-3 kb size fraction.

text: Late season sample

text: Leaf

characteristic: organ

text: Vitis vinifera

text: SfiI

text: SfiI

text: hermaphrodite

text: Cabernet Sauvignon Leaf - CA12LI

text: Plasmid

text: pDNR


text: UC Davis Department of Plant Pathology

text: live

text: Generic

characteristic: NCBI submission package

text: Cabernet Sauvignon

text: CA22LI is a cDNA library of Cabernet Sauvignon leaves. The leaves were collected on September 20, 2001, in Napa Valley, California, and represent leaves in late season development. These leaves were verified to be infected with the bacterial pathogen, Xylella fastidiosa, based on a diagnostic assay using PCR and Xylella-specific primer pairs. Plants were symptomatic at the time of collection. cDNA made by oligo-dT priming and directionally cloned. 5'and 3' adaptors were used in cloning as follows:5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' and 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)NN-3'. Library was constructed using the Clontech Creator SMART kit and size-selected to contain the 0.5-3 kb size fraction.

text: Late season sample

characteristic: dev-stage

text: Leaf

text: Vitis vinifera

characteristic: organism

text: SfiI

text: SfiI

text: hermaphrodite

text: Cabernet Sauvignon Leaf - CA22LI

characteristic: title

text: Plasmid

text: pDNR


characteristic: External Id

text: UC Davis Department of Plant Pathology

characteristic: INSDC center name

text: live

text: Generic

text: Cabernet Sauvignon

text: CA12EI is a cDNA library of Cabernet Sauvignon leaves. The leaves were collected on July 25, 2001, in Napa Valley, California, and represent leaves in mid-season development. These leaves were verified to be infected with the bacterial pathogen, Xylella fastidiosa, based on a diagnostic assay using PCR and Xylella-specific primer pairs. The plants were asymptomatic at the time of collection, but later developed symptoms. cDNAs were made by oligo-dT priming and directionally cloned. 5'and 3' adaptors were used in cloning as follows: 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' and 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)NN-3'. Library was constructed using the Clontech Creator SMART kit and size-selected to contain the 0.5-3 kb size fraction.

characteristic: description

text: Mid-season leaf material

text: Leaf

text: Vitis vinifera

text: SfiI

text: SfiI

text: hermaphrodite

text: Cabernet Sauvignon Leaf - CA12EI

text: Plasmid

text: pDNR


text: The Institute of Physical and Chemical Research (RIKEN)

text: live

text: Generic

text: B6-derived CD11 +ve dendritic cells

characteristic: cell type

text: Mus musculus

text: RIKEN full-length enriched, B6-derived CD11 +ve dendritic cells


text: live

text: Generic

text: Mus musculus

text: NOD

characteristic: strain

text: activated spleen

characteristic: tissue-type

text: RIKEN full-length enriched, activated spleen


text: live

text: Generic

text: erythroblast

text: Mus musculus

text: RIKEN full-length enriched, erythroblast


text: live

text: Generic

text: lymphocyte

text: Mus musculus

text: spleen

text: RIKEN full-length enriched, spleen lymphocyte


text: live

text: Generic

text: lymphocyte

text: Mus musculus

text: thymus

text: RIKEN full-length enriched, thymus lymphocyte


text: live

text: Generic

text: pooled tissues: (tissue_type=thymus,cell_type=lymphocyte), (tissue_type=spleen,cell_type=lymphocyte)

text: Mus musculus

text: RIKEN full-length enriched, pooled tissues, spleen and thymus lymphocytes


text: live

text: Generic

text: mast cells

text: Mus musculus

text: bone marrow

text: RIKEN full-length enriched, bone marrow mast cells


text: live

text: Generic

text: olfactory sensory neurons

text: Mus musculus

text: olfactory mucosa

text: RIKEN full-length enriched, olfactory mucosa olfactory sensory neurons


text: live

text: Generic

text: Mus musculus

text: visual cortex

text: RIKEN full-length enriched, visual cortex


text: Institute of Tropical Medicine

text: live

text: Generic

text: hemocytes

text: Insert was amplified using T3 and T7 vector specific primer; Total RNA was isolated from the hemocytes of unexposed Biomphalaria glabrata (13-16-R1) snails and a OneStep-RT-PCR was carried out using a pair of degenerate primers designed based on catalase consensus sequences and several fragments amplified in a differential display approach in our lab. The resulting PCR product was cloned into the plasmid vector pCR4-TOPO (Invitrogen,Groningen,Netherlands) and then subjected to sequencing.

text: adult

text: E.coli

characteristic: lab-host

text: Biomphalaria glabrata

text: hermaphrodite

characteristic: sex

text: Biomphalaria glabrata 13-16-R1 hemocytes catalase primers

text: Plasmid

characteristic: vector


text: West Virginia University

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: The lambda ZAP II cDNA (Stratagene) library was prepared from axenically cultured Neotyphodium coenophialum (ATCC 90664).

text: Mature

text: Epichloe coenophiala

text: Pseudostem

text: Neotyphodium coenophialum lambda ZAP II cDNA library


text: Washington University School of Medicine

text: live

text: Generic

characteristic: NCBI submission model

text: Library is oligo-dT primed and directionally cloned using cDNA prepared with the Stratagene cDNA synthesis kit. Tissue source: adult spleen. Library materials and construction by Greg Elgar (UK MRC HGMP-RC).

text: adult

text: DH10B (T1-resistant)

text: spleen

characteristic: organ

text: Takifugu rubripes

text: XhoI

text: EcoRI

text: Fugu hgmpB adult spleen

text: plasmid (ampicillin resistant)

text: pBluescript II KS (Stratagene)


text: live

text: Generic

characteristic: NCBI submission package

text: Library is oligo-dT primed and directionally cloned using cDNA prepared with the Stratagene cDNA synthesis kit. Tissue source: adult gill. Library materials and construction by Greg Elgar (UK MRC HGMP-RC).

text: adult

characteristic: dev-stage

text: DH10B (T1-resistant)

text: gill

text: Takifugu rubripes

text: XhoI

text: EcoRI

text: Fugu hgmpC adult gill

text: plasmid (ampicillin resistant)

text: pBluescript II KS (Stratagene)


text: live

text: Generic

text: Library is oligo-dT primed and directionally cloned using cDNA prepared with the Stratagene cDNA synthesis kit. Tissue source: adult gonad. Library materials and construction by Greg Elgar (UK MRC HGMP-RC).

text: adult

text: DH10B (T1-resistant)

text: gonad

text: Takifugu rubripes

characteristic: organism

text: XhoI

text: EcoRI

text: Fugu hgmpE adult gonad

characteristic: title

text: plasmid (ampicillin resistant)

text: pBluescript II KS (Stratagene)


characteristic: External Id

text: Scottish Crop Research Institute

characteristic: INSDC center name

text: live

text: Generic

text: Athyrium distentifolium

text: Fronds

text: Athyrium pSport1 cDNA library (EFlf01)

text: Plasmid


text: Stanford University

text: live

text: Generic

text: This library represents the unique genes found in the fourth round of EST sequencing at Stanford University for the maize genome project. Sequences are present from libraries 1091 and 3524. Contigs were assembled using ZmDBAssembler and 2 representatives from each contig were selected for the Unigene set. All singlets were also selected.

characteristic: description

text: Zea mays

text: 1114 - Unigene IV from Maize Genome Project


text: Stanford University

text: live

text: Generic

text: This library represents the unique genes found in the fifth round of EST sequencing at Stanford University for the maize genome project. Sequences are present from library 946. Contigs were assembled using ZmDBAssembler and 2 representatives from each contig were selected for the Unigene set. All singlets were also selected.

text: Zea mays

text: 1117 - Unigene V from Maize Genome Project


text: The Institute of Physical and Chemical Research (RIKEN)

text: live

text: Generic

text: pooled tissues: (dev_stage=adult,tissue_type=intestinal mucosa,sex=male),(dev_stage=adult,tissue_type=accessory axillary lymph node,sex=male),(dev_stage=adult, tissue_type=jejunal and colic lymph node,sex=male),(dev_stage=13 days embryo,tissue_type=brain,sex=mix),(dev_stage=13 days embryo,tissue_type=spinal cord,sex=mix),(dev_stage=15 days embryo,tissue_type=brain,sex=mix),(dev_stage=10 days pregnant, adult,tissue_type=ovary and uterus,sex=female),(dev_stage=8 days neonate,tissue_type=testis,sex=male),(dev_stage=16 days neonate,tissue_type=diencephalon,sex=male),(dev_stage=16 days neonate,tissue_type=medulla oblongata,sex=male),(dev_stage=21 days neonate,tissue_type=cerebellum,sex=mix),(dev_stage=1 month neonate,tissue_type=cerebellum,sex=mix), (dev_stage=0 day neonate,tissue_type=cortex,sex=mix), (dev_stage=adult,tissue_type=gall bladder,sex=male),(dev_stage=adult,tissue_type=vesicular gland,sex=male),(dev_stage=11 days embryo,tissue_type=spinal cord,sex=mix),(dev_stage=14 days embryo,tissue_type=lung,sex=mix)

text: Mus musculus

characteristic: strain

text: RIKEN full-length enriched, pooled tissues, adult intestinal mucosa, etc.


text: live

text: Generic

text: 14.5 days embryo RP+/+

text: Mus musculus

text: Rathke's pouches

characteristic: tissue-type

text: RIKEN full-length enriched, 14.5 days embryo RP+/+ Rathke's pouches


text: live

text: Generic

text: 12.5 days embryo

text: Mus musculus

text: Rathke's pouches

text: RIKEN full-length enriched, 12.5 days embryo Rathke's pouches


text: live

text: Generic

text: 14.5 days embryo df/df

text: Mus musculus

text: Rathke's pouches

text: RIKEN full-length enriched, 14.5 days embryo df/df Rathke's pouches


text: live

text: Generic

text: osteoclast-like cell

characteristic: cell type

text: Mus musculus

text: RIKEN full-length enriched, osteoclast-like cell


text: live

text: Generic

text: stroma cell

text: Mus musculus

text: RIKEN full-length enriched, stroma cell


text: live

text: Generic

text: synovial fibroblasts

text: Mus musculus

text: RIKEN full-length enriched, synovial fibroblasts


text: live

text: Generic

text: Mus musculus

text: whole joints

text: RIKEN full-length enriched, whole joints


text: live

text: Generic

text: 18-day embryo

text: Mus musculus

text: whole body

text: RIKEN full-length enriched, 18-day embryo whole body


text: live

text: Generic

text: 6 days embryo

text: Mus musculus

text: whole body

text: RIKEN full-length enriched, 6 days embryo whole body


text: live

text: Generic

text: 7 days embryo

text: Mus musculus

text: whole body

text: RIKEN full-length enriched, 7 days embryo whole body


text: live

text: Generic

text: 8 days embryo

text: Mus musculus

text: whole body

text: RIKEN full-length enriched, 8 days embryo whole body


text: live

text: Generic

text: 9 days embryo

text: Mus musculus

text: whole body

text: RIKEN full-length enriched, 9 days embryo whole body


text: Stanford University

text: live

text: Generic

characteristic: cultivar

text: RNA isolated by Hake lab. 1 million pfu amplified. Ampicillin is the selection marker.

text: 2 mm

text: E. coli XLOLR

characteristic: lab-host

text: ear

text: Zea mays

text: EcoRI

text: XhoI

text: ear

text: 3529 - 2 mm ear tissue from Schmidt and Hake labs

text: phagemid


text: University of Nevada

characteristic: INSDC first public

text: live

text: Generic

text: Library construction was performed according to Stratagene's recommended protocol for the Lambda UniZapXR vector and cDNA synthesis kit.

text: five-week-old

text: Mesembryanthemum crystallinum

text: EcoRI

text: XhoI

text: leaf

text: Ice plant Lambda Uni-Zap XR expression library, 5 days 0.5 M NaCl treatment, Crassulacean acid metabolism, phase I (2 AM).

text: Phagemid

text: Lambda Uni-Zap XR, Bluescript SK-

characteristic: vector


text: University of Nevada

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: five-week-old

text: Mesembryanthemum crystallinum

text: EcoRI

text: XhoI

text: leaf

text: Ice plant Lambda Uni-Zap XR expression library, 5 days 0.5 M NaCl treatment, Crassulacean acid metabolism, phase IV (5:30 PM).

text: Phagemid

text: Lambda Uni-Zap XR, Bluescript SK-


text: University of Nevada

text: live

text: Generic

characteristic: NCBI submission model

text: five-week-old

text: Mesembryanthemum crystallinum

text: EcoRI

text: XhoI

text: leaf

text: Ice plant Lambda Uni-Zap XR expression library, 5 days 0.5 M NaCl treatment, Crassulacean acid metabolism, phase II (8:00 AM).

text: Phagemid

text: Lambda Uni-Zap XR, Bluescript SK-


text: CEA/Saclay

text: live

text: Generic

characteristic: NCBI submission package

text: 30 months

text: Rattus norvegicus

text: female

characteristic: sex

text: WAG/Rij

text: kidney

text: Rattus norvegicus kidney WAG/Rij 30 months


text: Washington University School of Medicine

text: live

text: Generic

text: The library was constructed by Claire Murphy and Dr. James McCarter at Washington University, St. Louis. Oligo(dT)-SL1 PCR based library. cDNA PCR products of size >400 nucleotides containing SL1 on the 5' end and oligo(dT) on the 3' end were non-directionally cloned into pCRII-TOPO(Invitrogen) following the Topo TA cloning protocol. Trichinella spiralis adult worms were provided by Dr. Judith Appleton of Cornell Univeristy, Ithaca, NY (jaa2@cornell.edu).

text: adult

characteristic: dev-stage

text: Trichinella spiralis

characteristic: organism

text: EcoRI

text: EcoRI

text: whole organism

text: Trichinella spiralis adult SL1 TOPO v1

text: plasmid (kanamycin and ampicillin resistant)

text: pCRII-TOPO (Invitrogen)


characteristic: External Id

text: National Institute of Health

text: live

text: Generic

text: fibroblasts

text: young adult

text: Cavia porcellus

text: Hartley

text: Heart

text: Guinea pig heart fibroblasts

characteristic: title


text: Dept of Renewable Resources, University of Alberta

characteristic: INSDC center name

text: live

text: Generic

text: Pre germination

text: Pinus banksiana

text: Hind III

text: Hind III

text: Embryogenic Tissue and surrounding calli

text: Jack Pine pre-germination Salt Stress Responsive Gene Library

text: Plasmid

text: PCR Trap Vector


text: live

text: Generic

text: Pre germination

text: Picea mariana

text: Hind III

text: Hind III

text: Black Spruce pre-germination Salt Stress Responsive Gene Library

text: Plasmid

text: PCR Trap Vector


text: live

text: Generic

text: Post germination (6 weeks old seedlings)

text: Picea mariana

text: Hind III

text: Hind III

text: needles, stems, and roots

text: Black spruce 6 week old Salt Stress Responsive Gene Library

text: Plasmid

text: PCR Trap(Vector


text: live

text: Generic

text: Post germination (7 months-old-seedlings)

text: Pinus banksiana

text: Hind III

text: Hind III

text: needles and some stems

text: Jack pine 7 month old Salt Stress Responsive Gene Library

text: Plasmid

text: PCR Trap(Vector


text: Agriculture and Agri-Food Canada

text: live

text: Generic

text: SOLR

text: Solanum tuberosum

text: EcoRI

text: XhoI

text: Epidermis of enlarging tubers

text: LIPTS

text: pBluescript SK(-)


text: Agriculture and Agri-Food Canada

text: live

text: Generic

text: SOLR

text: Solanum tuberosum

text: EcoRI

text: XhoI

text: Leaves

text: LIPTL

text: pBluescript SK(-)


text: live

text: Generic

text: Library is oligo-dT primed and directionally cloned using cDNA prepared with the Stratagene cDNA synthesis kit. Tissue source: adult gut. Library materials and construction by Greg Elgar (UK MRC HGMP-RC).

characteristic: description

text: adult

text: DH10B (T1 phage-resistant)

text: gut

characteristic: organ

text: Takifugu rubripes

text: XhoI

text: EcoRI

text: Fugu hgmpD adult gut

text: plasmid (ampicillin resistant)

text: pBluescript II KS (Stratagene)


text: New Mexico State University

text: live

text: Generic

text: 6 hours after Phytophthora capsici inoculation

text: Root

text: Capsicum annuum

text: Xho 1

text: Criollo de Morelos 334 (CM334)

characteristic: strain

text: CM334 Root cDNA

text: Phage

text: Zap


text: New Mexico State University

text: live

text: Generic

text: drought-stressed

text: Root

text: Phaseolus acutifolius

text: Xho 1

text: tepary bean Root cDNA

text: Phage

text: Zap


text: live

text: Generic

text: The library was constructed by Claire Murphy and Dr. James McCarter at Washington University, St. Louis. Oligo(dT)-SL1 PCR based library. Embryo cDNA PCR products of size >400 nucleotides containing SL1 on the 5' end and oligo(dT) on the 3' end were non-directionally cloned into pCRII-TOPO(Invitrogen) following the Topo TA cloning protocol. 30-60 cell embryo material was provided by Dr. Richard Davis of City University of New York Graduate Center, College of Staten Island, Staten Island, NY (redavis@postbox.csi.cuny.edu).

text: 30-60 cell embryo

text: Ascaris suum

text: EcoRI

text: EcoRI

text: Ascaris suum embryo SL1 TOPO v1

text: pCRII-TOPO (Invitrogen)


text: US Department of Agriculture (USDA), ARS, PSI

text: live

text: Generic

text: Peking

characteristic: cultivar

text: cDNA clones from mRNA extracted from Peking roots 2 and 4 days past invasion.

text: Seedlings

text: Glycine max

text: Roots

characteristic: tissue-type

text: cDNA Peking library 2, 4 day SCN3

text: phagemid

text: pBluescript SK-


characteristic: INSDC first public

text: live

text: Generic

text: Peking

text: cDNA clones from mRNA extracted from Peking roots 6 and 8 days.

text: Seedlings

text: Glycine max

text: Roots

text: cDNA Peking library 6, 8 day SCN3

text: phagemid

text: pBluescript SK-


text: live

text: Generic

text: Peking

text: cDNA clones from mRNA extracted from roots of soybean cv. Peking 12 hrs after infection by SCN race 3. These are cloned in pBluescript SK- phagemid.

text: Seedlings

text: Glycine max

text: Roots

text: cDNA Peking library 12hr SCN3

text: phagemid

text: pBluescript SK-

characteristic: vector


text: CNRS, INSERM, ULP

characteristic: INSDC last update

text: live

text: Generic

text: Oligo dT cDNA library constructed from RNA of shield stage zebrafish embryos

text: shield stage

text: Danio rerio

text: NotI

text: SalI

text: oligo dT primed shield stage

text: pSPORT1 (BRL)


text: Guangdong Academy of Agricultural Sciences

text: live

characteristic: INSDC status

text: Generic

text: after inoculation with Magnaporthe grisea

text: Oryza sativa

text: rice cDNAs upregulated after M. grisea inoculation


text: Institute of Surgery

text: live

text: Generic

characteristic: NCBI submission model

text: Neonatal 5 days

text: Rattus norvegicus

text: male

text: Sprague-Dawley

text: Brain

text: Neonatal Rat Brain Express Library


text: Washington University School of Medicine

text: live

text: Generic

characteristic: NCBI submission package

text: The cDNA library was constructed by Keliang Tang, and Robert Cole at Washington University. cDNA was synthesized from poly(A)+ mRNA using an oligo-dT primer containing a XhoI site. Following second strand synthesis, EcoRI adapters were ligated to the cDNA and products were size-selected (Stratagene). The cDNAs were directionally cloned into the EcoRI (5' end)/XhoI (3' end) sites of the pZErO-2 vector (Invitrogen) and electroporated into DH10B cells (GeneHog, Invitrogen). WARNING: the library contains a small percentage of cDNAs derived from the human host cells.

text: Tachyzoite

text: DH10B DH10B (GeneHog, Research Genetics, Inc.)

characteristic: lab-host

text: Neospora caninum

text: EcoRI

text: XhoI

text: Nc-1 pZ Tachyzoite cDNA Library


text: Universidad de Cordoba

text: live

text: Generic

text: Cells induced in nitrate medium at continuous light and high CO2 for 1.5 hours.

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii EST isolated from nitrate induced cells


text: The Samuel Roberts Noble Foundation

text: live

text: Generic

text: Spores were isolated from sporocarps and underlying mycelia was discarded. Spores were washed thoroughly, surface sterilized and incubated at 4C. Total RNA was prepared and cDNA was synthesized using the Clontech Smart cDNA synthesis system. The cDNA was directionally ligated into Lambda TriplEX2 vector (Clontech) and packaged using Gigapack Gold packaging extracts. Plasmids (pTriplEX2) containing cDNA inserts were obtained from the recombinant lambda TriplEX2 phage via Cre-lox mediated conversion in BM25.8 cells (Clontech).

text: Spores isolated from sporocarps harvested from pot-cultures of Allium porum/Glomus versiforme growing in sand.

characteristic: dev-stage

text: SOLR

text: Diversispora versiformis

text: INVAM accession number IT104

text: Spores

text: GVSP

text: phagemid


text: National Institute of Genetics

text: live

text: Generic

text: Tokyo-kokei standard

text: Ipomoea nil

characteristic: organism

text: mixture of flower and flower bud

text: Ipomoea nil mixture of flower and flower bud


characteristic: External Id

text: RIKEN

text: live

text: Generic

text: Bos taurus

text: Bos taurus library (K Yokoyama)


text: RIKEN

text: live

text: Generic

text: Equus caballus

text: Equus caballus library (K Yokoyama)

characteristic: title


text: Universitee Libre de Bruxelles

characteristic: INSDC center name

text: live

text: Generic

text: Rattus norvegicus

text: Rattus norvegicus library (Quan X)


text: ICAT

text: live

text: Generic

text: country=China:Inner Mongolia

characteristic: description

text: Populus euphratica

text: root and shoot

text: Populus euphratica root and shoot


text: The Hospital for Sick Children, Department of Genetics

text: live

text: Generic

text: Homo sapiens

text: Eco RI

text: Eco RI

text: Homo sapiens library PCR 2.1 (Scherer SW)

text: PCR 2.1


text: Nanjing Agricultural University

text: live

text: Generic

text: These sequences were differentially expressed genes between biotype 1 and biotype 2 laboratory strains of Nilaparvata lugens which were got by suppression subtractive hybridization. They were identified by differential screen.

text: adult

text: rice varieties of TN1 and Mudgo

text: Nilaparvata lugens

text: female

characteristic: sex

text: biotype 1 and biotype 2 strains

text: Subtracted cDNA library between biotype 1 and biotype 2 strains of Nilaparvata lugens

text: plasmid

text: PGEM-T


text: University of Hohenheim, Institute of Phytomedicine

text: live

text: Generic

text: cDNA-AFLP technique. RNA was extracted from the abdomen of 2 d old adult Venturia canescens wasps and double-stranded cDNA was synthesized using the SMART PCR cDNA Synthesis Kit. The cDNA was digested with MseI, adapters were ligated to the termini, and two rounds of PCR were performed. On the first round the cDNAs were amplified with primers specific for the adapters. The products of this first round were amplified with primers based on the adapters containing one to three additional bases at their 3' end s.

text: adult

text: Venturia canescens

text: female

text: RP, RM (Beck et al, Insect Biochem Mol Biol, 29: 453-460, 1999)

characteristic: strain

text: abdomen


text: University of Bristol

text: live

text: Generic

text: neuronal, glial

characteristic: cell type

text: Rattus norvegicus, isolated from enriched library using suppressive subtractive hybridisation of supraoptic nucleus tissue; unknown function

text: adult 10 - 12 week

text: Rattus norvegicus

text: male

text: Sprague-Dawley

text: supraoptic nucleus

text: Rat supraoptic nucleus SSH cDNA library


text: National Institute on Aging/National Institutes of Health

text: live

text: Generic

text: Mouse cDNA project by the Laboratory of Genetics, National Institute on Aging (NIA), Intramural Research Program, NIH (http://lgsun.grc.nia.nih.gov/cDNA). This is a long-transcript enriched cDNA library (Ref. Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]). Total RNAs were obtained from Dr. Angelo L. Vescovi (Institute for Stem Cell Research, Italy). Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 2.0 Microgram of total RNA, treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-S. The products were purified by phenol/chloroform and Centricon 100. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pSPORT1 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 3.8 kb. The library was constructed by Yulan Piao.

text: Adult

text: Mus musculus

text: SalI

text: NotI

text: NIA Mouse Neural Stem Cell (Undifferentiated) cDNA Library (Long)

text: plasmid (ampicillin resistant)

text: pSPORT1 (Invitrogen)


text: live

text: Generic

text: Mouse cDNA project by the Laboratory of Genetics, National Institute on Aging (NIA), Intramural Research Program, NIH (http://lgsun.grc.nia.nih.gov/cDNA). This is a long-transcript enriched cDNA library (Ref. Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]). Total RNAs were obtained from Dr. Angelo L. Vescovi (Institute for Stem Cell Research, Italy). Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 2.0 Microgram of total RNA, treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-S. The products were purified by phenol/chloroform and Centricon 100. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pSPORT1 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 3.2 kb. The library was constructed by Yulan Piao.

text: Adult

text: Mus musculus

text: SalI

text: NotI

text: NIA Mouse Neural Stem Cell (Differentiated) cDNA Library (Long)

text: plasmid (ampicillin resistant)

text: pSPORT1 (Invitrogen)


text: Roche Bioscience

text: live

text: Generic

text: keratinocyte

text: HaCat cell line;derived from long term primary human adult skin keratinocyte

characteristic: cell-line

text: Matchmaker yeast two-hybrid sytem from Clontech; pACT2 vector has GAL4 AD and HA epitope under ADH1 promoter. This oligo-dT primed library was screened with human UbcH5a as bait, to obtained interacting proteins.

text: yeast/E.coli

text: Homo sapiens

text: EcoRI

text: XhoI

text: human keratinocyte matchmaker cDNA library

text: plasmid


text: Roche Bioscience

characteristic: INSDC first public

text: live

text: Generic

text: B cell

text: EBV-transformed human peripheral blood lymphocyte; B cell population; IG (+)

text: Matchmaker yeast two-hybrid sytem from Clontech;pACT has GAL4 AD under ADH1 promoter. This oligo-dT primed library was screened with human UbcH5a as bait, to obtain interacting proteins.

text: yeast/E.coli

text: Homo sapiens

text: XhoI

text: XhoI

text: human lymphocyte Matchmaker cDNA library

text: plasmid

text: pACT


text: Lethbridge Research Centre, AAFC

text: live

text: Generic

text: Vegetative growth

text: Tetrahymena pyriformis

text: Tp cDNA

text: Plasmid

text: pGEM-T

characteristic: vector


text: The Third Military Medical University

characteristic: INSDC last update

text: live

text: Generic

text: lung adenocarcinoma cell line

text: These sequences were differentially expressed genes between SPC-A-1/CDDP and its parental cell line which were got by suppression subtractive hybridization. They were identified by Northern blot.

text: Homo sapiens

text: lung cancer

characteristic: tissue-type

text: Human lung adenocarcinoma cell line SPC-A-1/CDDP SSH cDNA library


text: University of Minnesota

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: cultivar

text: cDNA was prepared from polyA+ enriched RNA. The cDNA was directionally ligated into the Unizap XR vector from Stratagene and packaged using Gigapack III Gold packaging extracts. Plasmids containing cDNA inserts were excised from the recombinant lambda-Zap phage using Ex-assist helper phage and propagated in XLOLR cells.

text: various stages

text: XLOLR

text: Medicago truncatula

text: EcoRI

text: XhoI

text: mixed tissues

text: MTUS

text: phagemid

text: pBluescript SK-


text: Life Sciences School, SOOCHOW University

text: live

text: Generic

characteristic: NCBI submission package

text: These sequences obtained by SSH and subtractive hybridisations generating populations of gene fragments are differentially expressed in hyperlipidemia rat

text: adult

characteristic: dev-stage

text: Rattus norvegicus

text: male

text: Sprague-Dawley

text: aorta

text: Subtracted cDNA library of aorta between hyperlipidemia and normal rat


characteristic: External Id

text: live

text: Generic

text: These sequences obtained by SSH and subtractive hybridisations generating populations of gene fragments were differentially expressed in hyperlipidemia rat

text: adult

text: Rattus norvegicus

characteristic: organism

text: male

text: Sprague-Dawley

text: blood cell

text: Subtracted cDNA library of aorta between hyperlipidemia and normal rat - blood cell


text: Michigan Technological University, School of Forest Resources & Environmental Science

characteristic: INSDC center name

text: live

text: Generic

text: leaf

characteristic: organ

text: Populus tremuloides

text: Aspen leaf cDNA Library

characteristic: title


text: live

text: Generic

text: root

text: Populus tremuloides

text: Aspen root cDNA Library


text: live

text: Generic

text: apex

text: Populus tremuloides

text: Aspen apex cDNA Library


text: live

text: Generic

text: stem

text: Populus tremuloides

text: Aspen stem cDNA Library


text: live

text: Generic

text: Non-normalized full-length enriched library from pooled mouse embryonic limb, maxilla and mandible, day 10.5 and 11.5 (size selected for the 0.5-1 kb fragments) Cloned directionally, priming method: Oligo-dT. cDNA enrichment: >1k bp, Average insert size 1.8k bp. Priming sequence: 5'GACTAGTTCTAGATCGCGAGCGGCCGCCC(T) 3'. Tissue contributed by, David Rowe. Library constructed by ResGen, Invitrogen Corp.

characteristic: description

text: DH10B (phage-resistant)

characteristic: lab-host

text: Mus musculus

text: EcoRV

text: NotI

text: phagemid (ampicillin resistant)


text: Washington University School of Medicine

text: live

text: Generic

text: This library was generated by cloning cDNAs directionally into Uni-ZAP(Stratagene) (T3 primer/EcoRI are at the 5'-end and T7/XhoI are at the 3'-end). The library was excised [now in pBluescript SK(+)] and normalized (Bonaldo et al 1996 Genome Research 6:791-806). Library constructed by Thomas Baum (tbaum@iastate.edu), Iowa State University, Plant Pathology Department and Jeff McDermott (jpmcderm@iastate.edu).

text: egg

text: Heterodera glycines

text: XhoI

text: EcoRI

text: mixed

characteristic: sex

text: whole organism

text: Heterodera glycines egg

text: plasmid (Ampicillin)

text: pBluescript SK+ (Stratagene)


characteristic: INSDC first public

text: live

text: Generic

text: 4th stage juvenile

text: Heterodera glycines

text: XhoI

text: EcoRI

text: mixed

text: whole organism

text: Heterodera glycines J4

text: plasmid (Ampicillin)

text: pBluescript SK+ (Stratagene)


text: live

text: Generic

text: adult

text: Heterodera glycines

text: XhoI

text: EcoRI

text: female

text: whole organism

text: Heterodera glycines virgin female

text: plasmid (Ampicillin)

text: pBluescript SK+ (Stratagene)

characteristic: vector


characteristic: INSDC last update

text: live

text: Generic

text: Library prepared by restriction digestion following methylation as described by Ardeshir F, Flint JE, Reese RT., in Proc Natl Acad Sci U S A 1985 Apr;82(8):2518-22.

text: late erythrocytic-stage parasite mRNA

text: E. coli

text: Plasmodium falciparum

text: EcoRI

text: Sal I

text: Honduras isolate

characteristic: strain

text: Plasmodium falciparum cDNA pUC8 Express Library


text: National Eye Institute

text: live

characteristic: INSDC status

text: Generic

text: A pBluescipt library was created by mass excision of a lacrimal gland library in LambdaZap (Sanghi S. et al, J Mol Biol 2001 Jun 29;310(1):127-39). EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC) as part of the NEIBank project.

text: Adult

text: Eye

text: Homo sapiens

text: lacrimal gland

characteristic: tissue-type

text: Human lacrimal gland: ni

text: Plasmid

text: pBluescript II


text: Harbin Institute of Technology

text: live

text: Generic

characteristic: NCBI submission model

text: mycelium

text: Chaetomium globosum

text: Chaetomium cDNA library


text: INSERM U433 , Faculte Medecine Laennec

text: live

text: Generic

characteristic: NCBI submission package

text: Rattus norvegicus

text: female

text: Wistar

text: hypothalamus

text: Rattus norvegicus hypothalamus Wistar


text: live

text: Generic

text: The cDNA library was constructed by Keliang Tang, and Robert Cole at Washington University. cDNA was synthesized from poly mRNA using an oligo-dT primer containing a XhoI site. Following second strand synthesis, EcoRI adapters were ligated to the cDNA, and products were size-selected on sephacryl S500. The cDNA were directionally cloned into the EcoRI/XhoI prepared Uni-ZAP XR lambda vector (Stratagene). Clones were converted to phagemids by mass excision using ExAssist helper phage and E.coli SOLR cell (Stratagene). The library may contain a small percentage of host or bacterial contaminants. Library materials provided by: David Sibley, Washington University.

text: Tachyzoite

characteristic: dev-stage

text: E.coli SOLR

text: Toxoplasma gondii

text: EcoRI

text: XhoI

text: TgME49 B7 Tachyzoite cDNA Library 1

text: plasmid

text: pBluescript SK-


characteristic: External Id

text: live

text: Generic

text: The library was constructed by Claire Murphy and Dr. James McCarter at Washington University, St. Louis. Oligo(dT)-SL2 PCR based library. cDNA PCR products of size >400 nucleotides containing SL2 on the 5' end and oligo(dT) on the 3' end were non-directionally cloned into pCRII-TOPO(Invitrogen) following the Topo TA cloning protocol. Intestine RNA was provided by Dr. Douglas Jasmer of Washington State University (djasmer@vetmed.wsu.edu).

text: Haemonchus contortus

text: EcoRI

text: EcoRI

text: intestine

text: Haemonchus contortus intestine SL2 TOPO v1

text: plasmid (kanamycin and ampicillin resistant)

text: pCRII-TOPO (Invitrogen)


text: live

text: Generic

text: The library was constructed by Claire Murphy and Dr. James McCarter at Washington University, St. Louis. Oligo(dT)-SL2 PCR based library. cDNA PCR products of size >400 nucleotides containing SL2 on the 5' end and oligo(dT) on the 3' end were non-directionally cloned into pCRII-TOPO(Invitrogen) following the Topo TA cloning protocol. Whole worm RNA was provided by Dr. Douglas Jasmer of Washington State University (djasmer@vetmed.wsu.edu).

text: Haemonchus contortus

characteristic: organism

text: EcoRI

text: EcoRI

text: whole organism

text: Haemonchus contortus whole worm SL2 TOPO v1

text: pCRII-TOPO (Invitrogen)


characteristic: INSDC center name

text: live

text: Generic

text: The cDNA library was constructed by Keliang Tang, and Robert Cole at Washington University. cDNA was synthesized from poly mRNA using an oligo-dT primer containing a XhoI site. Following second strand synthesis, EcoRI adapters were ligated to the cDNA, and products were size-selected on sephacryl S500. The cDNA were directionally cloned into the EcoRI/XhoI prepared pBluescript II SK+ vector, and transformed into XL10 cells (Stratagene). The library may contain a small percentage of host or bacterial contaminants.

text: Tachyzoite

text: E.coli XL10

text: Toxoplasma gondii

text: XhoI

text: EcoRI

text: TgME49 B7 Tachyzoite cDNA Library 2

text: plasmid

text: pBluescript II SK+


text: University of Liverpool

text: live

text: Generic

text: Normalized cDNA library prepared from liver of warm, cold and hypoxia challenged animals

text: Adult

text: E.coli Electromax DH10B

text: Cyprinus carpio

text: Sfi1 GGCCATTACGGCC

text: Sfi1 GGCCGCCTCGGCC

text: Male & female

text: Liver

text: Carp liver library 1

text: Plasmid


text: University of Liverpool

text: live

text: Generic

text: Normalized and serially subtracted cDNA library prepared from liver of warm, cold and hypoxia challenged animals

text: Adult

text: E.coli Electromax DH10B

text: Cyprinus carpio

text: Sfi1 GGCCATTACGGCC

text: Sfi1 GGCCGCCTCGGCC

text: Male & female

text: Liver

text: Carp liver library 2

text: Plasmid


text: University of Liverpool

text: live

text: Generic

text: Adult

text: E.coli Electromax DH10B

text: Cyprinus carpio

text: Sfi1 GGCCATTACGGCC

text: Sfi1 GGCCGCCTCGGCC

text: Male & female

text: Liver

text: Carp liver library 3

text: Plasmid


text: University of Liverpool

text: live

text: Generic

text: Normalized cDNA library prepared from mixed tissues of warm, cold and hypoxia challenged animals

text: Adult

text: E.coli Electromax DH10B

text: Cyprinus carpio

text: Sfi1 GGCCATTACGGCC

text: Sfi1 GGCCGCCTCGGCC

text: Male & female

text: Skeletal white muscle, cardiac muscle, kidney, brain, gill, intestinal mucosa

text: Carp mixed tissue library 1

text: Plasmid


text: University of Liverpool

text: live

text: Generic

text: Normalized and serially subtracted cDNA library prepared from mixed tissues of warm, cold and hypoxia challenged animals

text: Adult

text: E.coli Electromax DH10B

text: Cyprinus carpio

text: Sfi1 GGCCATTACGGCC

text: Sfi1 GGCCGCCTCGGCC

text: Male & female

text: Carp mixed tissue library 2

text: Plasmid


text: University of Liverpool

text: live

text: Generic

text: Adult

text: E.coli Electromax DH10B

text: Cyprinus carpio

text: Sfi1 GGCCATTACGGCC

text: Sfi1 GGCCGCCTCGGCC

text: Male & female

text: Carp mixed tissue library 3

text: Plasmid


text: University of Liverpool

text: live

text: Generic

text: Adult

text: E.coli Electromax DH10B

text: Cyprinus carpio

text: Sfi1 GGCCATTACGGCC

text: Sfi1 GGCCGCCTCGGCC

text: Male & female

text: Carp mixed tissue library 4

text: Plasmid


text: University of Liverpool

text: live

text: Generic

text: cDNA library prepared from liver of warm, cold and hypoxia challenged animals. cDNA was captured by hybridization to biotin-labeled subtracted cDNA fragments enriched for cold-induced transcripts

text: Adult

text: E.coli Electromax DH10B

text: Cyprinus carpio

text: Sfi1 GGCCATTACGGCC

text: Sfi1 GGCCGCCTCGGCC

text: Male & female

text: Liver

text: Carp liver library 4

text: Plasmid


text: University of Liverpool

text: live

text: Generic

text: Serially subtracted cDNA library prepared from muscle of warm, cold and hypoxia challenged animals

text: Adult

text: E.coli Electromax DH10B

text: Cyprinus carpio

text: Sfi1 GGCCATTACGGCC

text: Sfi1 GGCCGCCTCGGCC

text: Male & female

text: Muscle

text: Carp muscle library 1

text: Plasmid


text: University of Liverpool

text: live

text: Generic

text: Serially subtracted cDNA library prepared from liver of warm, cold and hypoxia challenged animals

text: Adult

text: E.coli Electromax DH10B

text: Cyprinus carpio

text: Sfi1 GGCCATTACGGCC

text: Sfi1 GGCCGCCTCGGCC

text: Male & female

text: Liver

text: Carp liver library 5

text: Plasmid


text: University of Liverpool

text: live

text: Generic

text: Serially subtracted cDNA library prepared from mixed tissues of warm, cold and hypoxia challenged animals

text: Adult

text: E.coli Electromax DH10B

text: Cyprinus carpio

text: Sfi1 GGCCATTACGGCC

text: Sfi1 GGCCGCCTCGGCC

text: Male & female

text: Liver, skeletal white muscle, cardiac muscle, kidney, brain, gill, intestinal mucosa

text: Carp mixed tissue library 5

text: Plasmid


text: University of Liverpool

text: live

text: Generic

text: cDNA library prepared from muscle of warm, cold and hypoxia challenged animals. cDNA was captured by hybridization to biotin-labeled subtracted cDNA fragments enriched for hypoxia-regulated transcripts

text: Adult

text: E.coli Electromax DH10B

text: Cyprinus carpio

text: Sfi1 GGCCATTACGGCC

text: Sfi1 GGCCGCCTCGGCC

text: Male & female

text: Muscle

text: Carp muscle library 2

text: Plasmid


text: University of Liverpool

text: live

text: Generic

text: cDNA library prepared from mixed tissues of warm, cold and hypoxia challenged animals. cDNA was captured by hybridization to biotin-labeled subtracted cDNA fragments enriched for hypoxia-regulated transcripts

text: Adult

text: E.coli Electromax DH10B

text: Cyprinus carpio

text: Sfi1 GGCCATTACGGCC

text: Sfi1 GGCCGCCTCGGCC

text: Male & female

text: Carp mixed tissue library 6

text: Plasmid


text: Colorado State University

text: live

text: Generic

text: Messenger RNA was extracted from 250 mg of females (AIDL strain) using Oligotex Direct mRNA resin (QIAGEN Inc., Valencia CA). The SMART PCR cDNA synthesis Kit (Qiagen) was used to synthesize and amplify cDNAs from the mRNA. These were cloned into pCR2.1 (Invitrogen Corp., Carlsbad, CA) for sequencing.

text: nulliparous

text: Ochlerotatus triseriatus

text: female

text: AIDL strain

text: whole body

text: Nulliparous Female Ochlerotatus triseriatus


text: Colorado State University

text: live

text: Generic

text: Messenger RNA was extracted from 250 mg of fourth instar larvae (AIDL strain) using Oligotex Direct mRNA resin (QIAGEN Inc., Valencia CA). The SMART PCR cDNA synthesis Kit (Qiagen) was used to synthesize and amplify cDNAs from the mRNA. These were cloned into pCR2.1 (Invitrogen Corp., Carlsbad, CA) for sequencing.

text: 4rth instar larvae

text: Ochlerotatus triseriatus

text: AIDL strain

text: whole body

text: 4rth instar larval Ochlerotatus triseriatus


text: Colorado State University

text: live

text: Generic

text: Messenger RNA was extracted from ovaries from 50 twice parous females (AIDL strain) using Oligotex Direct mRNA resin (QIAGEN Inc., Valencia CA). The SMART PCR cDNA synthesis Kit (Qiagen) was used to synthesize and amplify cDNAs from the mRNA. These were cloned into pCR2.1 (Invitrogen Corp., Carlsbad, CA) for sequencing.

text: twice parous females

text: Ochlerotatus triseriatus

text: AIDL strain

text: whole body

text: twice parous female Ochlerotatus triseriatus


text: Medical University of South Carolina

text: live

text: Generic

text: circulating hemocytes

characteristic: cell type

text: Subtractive hybridization was performed with hemocyte cDNA prepared from control and WSSV-infected shrimp, 9 hours post infection.

text: adult

text: DH5 alpha

text: hemolymph

characteristic: organ

text: Penaeus vannamei

text: EcoRI

text: EcoRI

text: hemolymph

text: plasmid


text: Medical University of South Carolina

text: live

text: Generic

text: circulatory hemocytes

text: 80 highly abundant cDNAs were depleted from normal hemocyte cDNA to generate a low-redundancy library for EST collection

characteristic: description

text: adult

text: DH5 alpha

text: hemolymph

text: Penaeus vannamei

text: SfiIA

text: SfiIB

text: hemolymph

characteristic: title

text: plasmid


text: Beijing AgroBiotechnology Research Center

text: live

text: Generic

text: cDNA library was constructed by SUPERSCRIPTTM Lambda System for cDNA Synthesis and Cloning (GIBCOBRL,CAT.NO.19643-014)

text: folding stage

text: E.coli DH10B(ZIP)

characteristic: lab-host

text: Brassica rapa subsp. pekinensis

text: NotI

text: SalI

text: heading leaf

text: GIBCOBRL CAT. NO. 19643-014


text: University of Georgia

characteristic: INSDC first public

text: live

text: Generic

characteristic: cultivar

text: Gossypium barbadense

text: Leaves

text: CMS

text: Phage

text: Lambda DASHII


text: University of Georgia

characteristic: INSDC last update

text: live

text: Generic

text: Accession 'T25'

text: solR

text: Gossypium hirsutum

text: Leaves

text: pAR

text: Phagemid

text: lambdaZAP

characteristic: vector


text: University of Georgia

text: live

characteristic: INSDC status

text: Generic

text: Pima S6

text: Gossypium barbadense

text: Leaves

text: pBAM

text: Plasmid


text: University of Georgia

text: live

text: Generic

text: Accession 'TM-1'

text: Gossypium hirsutum

text: Leaves

characteristic: tissue-type

text: pGH

text: Plasmid


text: University of Georgia

text: live

text: Generic

characteristic: NCBI submission model

text: Accession 'A1-73'

text: Gossypium herbaceum subsp. africanum

text: Leaves

text: Pst1-A fragment

text: Plasmid


text: University of Georgia

text: live

text: Generic

characteristic: NCBI submission package

text: Gossypium raimondii

text: Leaves

text: Pst1-G fragment

text: Plasmid


text: University of Georgia

text: live

text: Generic

text: Accession 'TM-1'

text: Gossypium hirsutum

text: Leaves

text: Pst1-M fragment

text: Plasmid


text: University of Georgia

text: live

text: Generic

text: Accession 'TM-1'

text: Gossypium hirsutum

text: Leaves

text: Pst1-P fragment

text: Plasmid


text: University of Georgia

text: live

text: Generic

text: Tamcot GNCH

text: Gossypium hirsutum

text: Sall

text: Leaves

text: pVNC

text: Plasmid


text: University of Georgia

text: live

text: Generic

text: Pima S6

text: Gossypium barbadense

text: EcoRV

text: Leaves

text: PXP

text: Plasmid

text: pBLUESCRIPT KS(+)


text: University of Bergen

text: live

text: Generic

text: cDNA library from sea urchin embryos, made in a plasmid vector and arrayed in the laboratory of E.H. Davidson (Caltech, USA)

text: 40 h embryo

characteristic: dev-stage

text: Strongylocentrotus purpuratus

text: Mixed

characteristic: sex

text: 40 h embryonic cDNA library (arrayed)

text: Plasmid


characteristic: External Id

text: Auburn University, Cellular and Molecular Biosciences Program

text: live

text: Generic

text: cDNA generated by RT-PCR from total RNA extracted from porcine neonatal uterine tissue.

text: neonatal day 14

text: uterus

text: Sus scrofa

characteristic: organism

text: female

text: uterus

text: Sus scrofa neonatal uterus RT-PCR


text: Washington University School of Medicine

characteristic: INSDC center name

text: live

text: Generic

text: Toxoplasma RH strain tachyzoites were grown in human foreskin fibroblast cultures in vitro. The library was originally constructed by K.L.Wan, Cambridge University. cDNAs were synthesized from polyA RNAs by oligo d(T) priming and directionally cloned into the EcoRI to XhoI sites of the Lambda ZapII vector using the ZAP-cDNA synthesis kit (Stratagene). The primary cDNA library was mass excised as phagemid using ExAssist helper phage (Stratagene). Phagemid DNA was extracted by phenol-chloroform method, and hybridized against a pool of highly abundant genes which were derived from short-cycle PCR of the primary cDNA library. The normalized library was electroporated into DH10B (GeneHog, Invitrogen, Inc). WARNING: the library contains a small percentage of cDNAs derived from the human host cells.

text: Tachyzoite

text: DH10B (GeneHog, Invitrogen, Inc.)

text: Toxoplasma gondii

text: EcoRI

text: XhoI

text: RH (Type I)

characteristic: strain

text: TgRH Tachyzoite Norm 1 cDNA Library

text: pBluscript SK-


text: live

text: Generic

characteristic: description

text: Tachyzoite

text: DH10B (GeneHog, Invitrogen, Inc.)

text: Toxoplasma gondii

text: EcoRI

text: XhoI

text: RH (Type I)

text: TgRH Tachyzoite Norm 5 cDNA Library

text: pBluscript SK-


text: live

text: Generic

text: Tachyzoite

text: DH10B (GeneHog, Invitrogen, Inc.)

text: Toxoplasma gondii

text: EcoRI

text: XhoI

text: RH (Type I)

text: TgRH Tachyzoite Norm 7 cDNA Library

characteristic: title

text: pBluscript SK-


text: Emory University

text: live

text: Generic

text: The Rat Lambda Zap 8 h light-exposed retina cDNA library was constructed from retinal RNA isolated from weanling male albino Sprague-Dawley rats, which were maintained in darkness for 40 days. At 61 days of age rats were exposed to intense visible light (490-580 nm; 1200 to 1400 lux) for 8 hours.

text: adult

text: Rattus norvegicus

text: male

text: Sprague-Dawley

text: retina

text: Phage

text: Uni-ZAP XR


text: ZJU

text: live

text: Generic

text: Homo sapiens

text: colorectal adenocarcinoma

text: Human colorectal adenocarcinoma cDNA library


text: Justus-Liebig-University Giessen

text: live

text: Generic

text: Ingrid (Ingrid-WeihenstephanCP127422)

text: 7-day-old seedling

text: Hordeum vulgare

text: primary leaf

text: Hordeum vulgare primary leaf 7-day-old seedling


text: INSERM U433 , Faculte Medecine Laennec

text: live

text: Generic

text: country:France

text: Mus musculus

text: female

text: hypothalamus

text: Mus musculus female hypothalamus Lambda Triplex brain library


text: Kagawa University

text: live

text: Generic

text: immature pollen grains cultured in a nutrient-free medium

characteristic: cell type

text: Samsun

text: Nicotiana tabacum

text: Nicotiana tabacum immature pollen grains


text: The Population Council

characteristic: INSDC first public

text: live

text: Generic

text: Rattus norvegicus

text: Rattus norvegicus mRNA regulated by estrogen


text: USDA, ARS, Animal and Natural Resources Institute

text: live

text: Generic

text: epithelium

text: This mammary-derived cDNA library was created as part of a collaborative project between the ARS Gene Evaluation and Mapping Laboratory and the EMBRAPA Dairy Cattle Research Center under the sponsorship of USDA, ARS/EMBRAPA-LABEX program in animal genomics.RNA extracted on 12/18/00, RT with Superscript II at 37 deg C annealing temperature.

text: prepubertal

characteristic: lab-host

text: mammary

characteristic: organ

text: Bos taurus

text: SmaI

text: SmaI

text: female

text: Holstein

text: mammary

text: BARC-EMBRAPA 300BOV

text: plasmid


text: Chinju National University

characteristic: INSDC last update

text: live

text: Generic

text: average insert size:1000 bp;initial pfu:2 x 10(6)

text: Sus scrofa

text: muscle

text: Porcine Muscle Lambda cDNA


text: Institute of Cancer Research

text: live

characteristic: INSDC status

text: Generic

text: testicular germ cell

characteristic: cell-line

text: Homo sapiens

text: Homo sapiens GCT27


text: University of Nevada

text: live

text: Generic

characteristic: NCBI submission model

text: mixed; 8, 9, 11, 13, 15, 16 weeks daf

text: Vitis vinifera

text: EcoRI

text: XhoI

text: berries

characteristic: tissue-type

text: An expressed sequence tag database for abiotic stressed berries of Vitis vinifera var. Chardonnay

text: Phagemid

text: Lambda Uni-Zap XR, Bluescript SK-

characteristic: vector


text: UMR INRA/Universite Henri Poincare 1136

text: live

text: Generic

characteristic: NCBI submission package

characteristic: cultivar

text: Laccaria bicolor Maire Orton; cDNA library from mycelium of Laccaria bicolor grown for ten weeks on agar-plates containing low sugar (1 g/l glucose) Pachlewski medium. The cDNA library was constructed from mRNA which was isolated from 13ug of total Dnase digested RNA using the SMART cDNA synthesis kit (Clontech, Palo Alto, CA, USA) according to the manufacturer's instructions. The resulting cDNA was packed into lambda phages using the Gigapack III Gold packaging kit (Stratagene, La Jolla, CA). The pTriplEx2 phagemid clones in Escherichia choli were obtained by using the mass in vivo excision protocol according to the manufacturer's instructions (Clontech).

text: ten-weeks-old

characteristic: dev-stage

text: mycelium

text: Laccaria bicolor


characteristic: External Id

text: UMR INRA/Universite Henri Poincare 1136

characteristic: INSDC center name

text: live

text: Generic

text: Pisolithus microcarpus Coker & Mass. Cunn. nom prov.; cDNA library from mycelium of Pisolithus microcarpus grown for three weeks on agar-plates containing low sugar (1 g/l glucose) Pachlewski medium. The cDNA library was constructed from 1 ug of total RNA using the SMART cDNA synthesis kit (Clontech, Palo Alto, CA, USA) according to the manufacturer's instructions. The resulting cDNA was packed into lambda phages using the Gigapack III Gold packaging kit (Stratagene, La Jolla, CA). The pTriplEx2 phagemid clones in Escherichia choli were obtained by using the mass in vivo excision protocol according to the manufacturer's instructions (Clontech).

text: three-weeks-old

text: mycelium

text: Pisolithus microcarpus

characteristic: organism


text: University of Hohenheim, Institute of Phytomedicine

text: live

text: Generic

text: Florina

text: cDNA-AFLP technique. RNA was extracted from the Malus domestica leaves and double-stranded cDNA was synthesized using the SMART PCR cDNA Synthesis Kit. The cDNA was digested with MseI, adapters were ligated to the termini, and two rounds of PCR were performed. On the first round the cDNAs were amplified with primers specific for the adapters. The products of this first round were amplified with primers based on the adapters containing one to three additional bases at their 3' ends.

characteristic: description

text: one year old trees

text: leaf

text: Malus domestica

characteristic: title


text: University of California, Los Angeles, School of Medicine

characteristic: INSDC first public

text: live

text: Generic

text: bFGF Cultured Neurospheres - differentiated

characteristic: cell type

text: Cells were harvested from P0 mouse cortex and cultured for 4 weeks in the presence of bFGF to grow neurospheres. Half of the cells were then placed under differentiating conditions for 24 hours. RNA was extracted from both groups using acid phenol extraction, PolyA(+) RNA was purified using oligo dt paramagnetic beads, and cDNA was synthesized. Amplicons were created from Dpn II digested cDNA and 2 rounds of RDA subtraction were performed using amplicons from the undifferentiated cells as the driver. Resultant subtracted library, enriched in clones from the differentiated cells, was then shotgun cloned into BamH1 digested Bluescript SK II+ and transformed into E. coli.

text: Hbl electromax DH10B (GibcoBrl)

text: Brain

text: Mus musculus

text: BAMHI

characteristic: strain

text: Cerebral Cortex

text: differentiated neural progenitor enriched library

text: Plasmid

text: pBluescript KS II+


text: Murdoch Children's Research Institute and University of Melbourne

characteristic: INSDC last update

text: live

text: Generic

text: Embryonic mouse gonads were sexed by PCR and RNA pooled as either female or male. PolyA RNA was purified, reverse transcribed to cDNA, digested with RsaI, and female cDNA fragments were subtracted from male using the PCR-Select protocol (Clontech).

text: 12.0-12.5 dpc

text: gonad

characteristic: organ

text: Mus musculus

text: mixed

characteristic: sex

text: 12.0-12.5 dpc subtracted mouse testis

text: pGEM-T


text: CSIRO

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: The library was constructed from mRNA extracted from Glycine latifolia leaves with a leaf relative water content of 40-60%. The library was enriched for differentially expressed ESTs using the Clonetech PCR-Select cDNA Subtraction Kit. ESTs from the library were cloned into pCRII-TOPO using the Invitrogen TOPO TA Cloning kit. All submissions have been confirmed as drought-induced by real time PCR.

text: young fully expanded leaf

text: Glycine latifolia

text: Acc 521

text: leaf

text: Drought-induced cDNAs from Glycine latifolia leaves

text: Plasmid

text: Invitrogen's pCRII-TOPO