text: University of Edinburgh

text: live

text: Generic

text: Teladorsagia circumcincta is a parasitic nematode. The library was constructed from mRNA from Teladorsagia circumcincta L3 stage.

text: Teladorsagia circumcincta

text: EcoRI

text: XhoI

text: mixed

text: Teladorsagia circumcincta L3 library

text: phage

text: Lambda Uni-ZAP

characteristic: vector


status: PUBLIC

characteristic: External Id

text: INRA - SCRIBE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Clone distribution : AGENAE Resource centre. Francois PIUMI, Francois.Piumi@jouy.inra.fr, INRA, CEA Radiobiologie et Etude du genome (LREG), Domaine de Vilvert, 78352, Jouy-en-Josas cedex, France, +33 (0) 1.34.65.28.02, +33 (0) 1.34.65.22.73

characteristic: description

text: from embryos to adults

tag: attribute

characteristic: dev-stage

characteristic: lab-host

text: Oncorhynchus mykiss

characteristic: organism

text: adipose tissue, blood, brain, differentiating gonads, gills, interrenal, intestine, kidney, liver, muscle, ovary, pituitary, testis

characteristic: tissue-type

text: AGENAE Rainbow trout multi-tissues library (tcaa)

characteristic: title


text: INRA - SCRIBE

text: live

text: Generic

text: Oncorhynchus mykiss

text: adipose tissue, blood, brain, differentiating gonads, interrenal, intestine, kidney, liver, muscle, ovary, pituitary, testis

text: AGENAE Rainbow trout normalized multi-tissues library (tcad)


text: INRA - SCRIBE

text: live

text: Generic

text: juveniles

text: Oncorhynchus mykiss

text: testis

text: AGENAE Rainbow trout normalized testis library (tcab)


text: INRA - SCRIBE

text: live

text: Generic

text: from embryos to adults

text: Oncorhynchus mykiss

text: AGENAE Rainbow trout normalized multi-tissues library (tcac)


text: INRA - SCRIBE

text: live

text: Generic

text: Rainbow trout multi-tissues - normalized + 1 subtraction ; Clone distribution : AGENAE Resource centre. Francois PIUMI, Francois.Piumi@jouy.inra.fr, INRA, CEA Radiobiologie et Etude du genome (LREG), Domaine de Vilvert, 78352, Jouy-en-Josas cedex, France, +33 (0) 1.34.65.28.02, +33 (0) 1.34.65.22.73

text: from embryos to adults

text: Oncorhynchus mykiss

text: tcav


text: Washington University School of Medicine

text: live

text: Generic

text: 1st strand primed with an oligo (dT) primer: double stranded cDNA was cloned into the EcoRI and BamHI sites of the pBluescript SK+ vector. Primary library; non-amplified.

text: Anolis sagrei

text: EcoRI

text: BamHI

text: Anolis sagrei limb bud 1

text: Phagemid

text: Bluescript SK+


text: USDA, ARS, Animal and Natural Resources Institute

text: live

text: Generic

text: epithelium

characteristic: cell type

text: This mammary-derived cDNA library was created as part of a collaborative project between the ARS Gene Evaluation and Mapping Laboratory and the EMBRAPA Dairy Cattle Research Center under the sponsorship of USDA, ARS/EMBRAPA-LABEX program in animal genomics. RNA extracted on 12/18/00, RT with Superscript II at 37 deg C annealing temperature.

text: prepubertal

text: mammary

characteristic: organ

text: Bos taurus

text: SmaI

text: SmaI

text: female

characteristic: sex

text: Holstein

characteristic: strain

text: mammary

text: BARC-EMBRAPA 301BOV

text: plasmid


characteristic: External Id

text: live

text: Generic

text: epithelium

text: prepubertal

text: mammary

text: Bos taurus

text: SmaI

text: SmaI

text: female

text: Holstein

text: mammary

text: BARC-EMBRAPA 302BOV

text: plasmid

characteristic: vector


characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: epithelium

text: This mammary-derived cDNA library was created as part of a collaborative project between the ARS Gene Evaluation and Mapping Laboratory and the EMBRAPA Dairy Cattle Research Center under the sponsorship of USDA, ARS/EMBRAPA-LABEX program in animal genomics.RNA extracted on 12/18/00, RT with Superscript II at 37 deg C annealing temperature.

text: prepubertal

text: mammary

text: Bos taurus

text: SmaI

text: SmaI

text: female

text: Holstein

text: mammary

text: BARC-EMBRAPA 303BOV

text: plasmid


characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: epithelium

text: This mammary-derived cDNA library was created as part of a collaborative project between the ARS Gene Evaluation and Mapping Laboratory and the EMBRAPA Dairy Cattle Research Center under the sponsorship of USDA, ARS/EMBRAPA-LABEX program in animal genomics.RNA extracted on 12/18/00, RT with Superscript II at 37 deg C annealing temperature

text: prepubertal

text: mammary

text: Bos taurus

text: SmaI

text: SmaI

text: female

text: Holstein

text: mammary

text: BARC-EMBRAPA 304BOV

text: plasmid


text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: epithelium

text: prepubertal

text: mammary

text: Bos taurus

text: SmaI

text: SmaI

text: female

text: Holstein

text: mammary

text: BARC-EMBRAPA 305BOV

text: plasmid


text: live

text: Generic

text: epithelium

text: This mammary-derived cDNA library was created as part of a collaborative project between the ARS Gene Evaluation and Mapping Laboratory and the EMBRAPA Dairy Cattle Research Center under the sponsorship of USDA, ARS/EMBRAPA-LABEX program in animal genomics. RNA extracted on 6/8/02, RT Superscript II at 37 deg C, annealing temperature, PCR with 16mer mix of AM1-6.

characteristic: description

text: involuted

characteristic: dev-stage

text: mammary

text: Bos indicus

text: SmaI

text: SmaI

text: female

text: Brazilian Dairy Gir

text: teat cistern and alveoli

text: BARC-EMBRAPA 306BOV

text: plasmid


text: live

text: Generic

text: epithelium

text: This mammary-derived cDNA library was created as part of a collaborative project between the ARS Gene Evaluation and Mapping Laboratory and the EMBRAPA Dairy Cattle Research Center under the sponsorship of USDA, ARS/EMBRAPA-LABEX program in animal genomics. RNA extracted on 6/8/02, RT with Superscript II at 37 deg C annealing temperature, PCR with 16mer mix of AM1-6.

text: involuted

characteristic: lab-host

text: mammary

text: Bos indicus

characteristic: organism

text: SmaI

text: SmaI

text: female

text: Brazilian Dairy Gir

text: mammary

text: BARC-EMBRAPA 307BOV

text: plasmid


text: live

text: Generic

text: epithelium

text: This mammary-derived cDNA library was created as part of a collaborative project between the ARS Gene Evaluation and Mapping Laboratory and the EMBRAPA Dairy Cattle Research Center under the sponsorship of USDA, ARS/EMBRAPA-LABEX program in animal genomics. RNA extracted on 6/8/02, RT with Superscript II at 37 deg C annealing temperature, PCR with 16mer AM1.

text: involuted

text: mammary

text: Bos indicus

text: EcoRV

text: EcoRV

text: female

text: Brazilian Dairy Gir

text: mammary

characteristic: tissue-type

text: BARC-EMBRAPA 308BOV

characteristic: title

text: plasmid

text: pST BLUE1


text: live

text: Generic

text: epithelium

characteristic: cell type

text: This mammary-derived cDNA library was created as part of a collaborative project between the ARS Gene Evaluation and Mapping Laboratory and the EMBRAPA Dairy Cattle Research Center under the sponsorship of USDA, ARS/EMBRAPA-LABEX program in animal genomics. RNA extracted on 6/8/02, RT with Superscript II at 37 deg C annealing temperature, PCR with 16mer AM2.

text: involuted

text: mammary

characteristic: organ

text: Bos indicus

text: EcoRV

text: EcoRV

text: female

text: Brazilian Dairy Gir

text: mammary

text: BARC-EMBRAPA 309BOV

text: plasmid

text: pST BLUE1


text: live

text: Generic

text: multiple

text: This mammary-derived cDNA library was created as part of a collaborative project between the ARS Gene Evaluation and Mapping Laboratory and the EMBRAPA Dairy Cattle Research Center under the sponsorship of USDA, ARS/EMBRAPA-LABEX program in animal genomics. RNA extracted on 6/8/02 with Invitrogen Superscript II at 37 deg C annealing temperature.

text: involuted

text: mammary

text: Bos indicus

text: SmaI

text: SmaI

text: female

characteristic: sex

text: Brazilian Dairy Gir

characteristic: strain

text: teat cistern and alveoli

text: BARC-EMBRAPA 312BOV

text: plasmid


characteristic: External Id

text: live

text: Generic

text: epithelium

text: This mammary-derived cDNA library was created as part of a collaborative project between the ARS Gene Evaluation and Mapping Laboratory and the EMBRAPA Dairy Cattle Research Center under the sponsorship of USDA, ARS/EMBRAPA-LABEX program in animal genomics. RNA extracted on 6/8/02, RT with Superscript II at 37 deg C annealing temperature, PCR with 16mer AM3.

text: involuted

text: mammary

text: Bos indicus

text: EcoRV

text: EcoRV

text: female

text: Brazilian Dairy Gir

text: mammary

text: BARC-EMBRAPA 311BOV

text: plasmid

text: pST BLUE1

characteristic: vector


characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: multiple

text: This mammary-derived cDNA library was created as part of a collaborative project between the ARS Gene Evaluation and Mapping Laboratory and the EMBRAPA Dairy Cattle Research Center under the sponsorship of USDA, ARS/EMBRAPA-LABEX program in animal genomics.RNA extracted on 6/8/02, RT with Superscript II at 37 deg C annealing temperature.

text: involuted

text: mammary

text: Bos indicus

text: HincII

text: HincII

text: female

text: Brazilian Dairy Gir

text: teat cistern and alveoli

text: BARC-EMBRAPA 313BOV

text: plasmid


characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: epithelium

text: This mammary-derived cDNA library was created as part of a collaborative project between the ARS Gene Evaluation and Mapping Laboratory and the EMBRAPA Dairy Cattle Research Center under the sponsorship of USDA, ARS/EMBRAPA-LABEX program in animal genomics. RNA extracted on 6/8/02, RT with Superscript II at 37 deg C annealing temperature, PCR with 16mer AM5 only.

text: involuted

text: mammary

text: Bos indicus

text: HincII

text: HincII

text: female

text: Brazilian Dairy Gir

text: teat cistern

text: BARC-EMBRAPA 314BOV

text: plasmid


text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: epithelium

text: This mammary-derived cDNA library was created as part of a collaborative project between the ARS Gene Evaluation and Mapping Laboratory and the EMBRAPA Dairy Cattle Research Center under the sponsorship of USDA, ARS/EMBRAPA-LABEX program in animal genomics. RNA extracted on 6/8/02, RT with Superscript II at 37 deg C annealing temperature, PCR with 16mer AM5.

text: involuted

text: mammary

text: Bos indicus

text: HincII

text: HincII

text: female

text: Brazilian Dairy Gir

text: mammary

text: BARC-EMBRAPA 315BOV

text: plasmid


text: live

text: Generic

text: epithelium

text: This mammary-derived cDNA library was created as part of a collaborative project between the ARS Gene Evaluation and Mapping Laboratory and the EMBRAPA Dairy Cattle Research Center under the sponsorship of USDA, ARS/EMBRAPA-LABEX program in animal genomics. RNA extracted on 6/8/02, RT with Superscript II at 37 deg C, annealing temperature, PCR with 16mer AM5.

characteristic: description

text: involuted

characteristic: dev-stage

text: mammary

text: Bos indicus

text: SmaI

text: SmaI

text: female

text: Brazilian Dairy Gir

text: mammary

text: BARC-EMBRAPA 316BOV

text: plasmid


text: live

text: Generic

text: epithelium

text: involuted

characteristic: lab-host

text: mammary

text: Bos indicus

characteristic: organism

text: HincII

text: HincII

text: female

text: Brazilian Dairy Gir

text: mammary

text: BARC-EMBRAPA 317BOV

text: plasmid


text: University of Edinburgh

text: live

text: Generic

text: Teladorsagia circumcincta is a parasitic nematode. The library was constructed by Dr Diane Redmond and Dr David Knox, Moredun Research Institute, Midlothian, UK.from mRNA from Teladorsagia circumcincta L4 stage.

text: Teladorsagia circumcincta

text: EcoRI

text: XhoI

text: mixed

text: Teladorsagia circumcincta L4 library

characteristic: title

text: phage

text: Lambda Uni-ZAP


text: Universita degli Studi dell'Insubria

text: live

text: Generic

text: Dicentrarchus labrax

text: brain and liver

characteristic: tissue-type

text: cDNA from differential display on sea bass (Dicentrarchus labrax) farmed at different population density


text: live

text: Generic

text: 1st strand cDNA was primed with a NotI- oligo(dT) primer 5'-AACTGGAAGAATTCGCGGCCGCTGTACCGATGTTTTTTTTTTTTTTTTTTT-3'; double- stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library went through one round of normalization, and was constructed in the laboratory of M. Bento Soares (University of Iowa).

text: juvenile, 13-15 days

text: DH10B (phage-resistant)

text: brain

characteristic: organ

text: Mus musculus

text: NotI

text: EcoRI

text: pituitary gland

text: Soares NMBP13-15

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: 1st strand cDNA was primed with a NotI - oligo(dT) primer 5'-AACTGGAAGAATTCGCGGCCGCGAATAATACATTTTTTTTTTTTTTTTTTT-3'; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3'(Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacII vector. Library went through one round of normalization, and was constructed in the laboratory of M. Bento Soares (University of Iowa).

text: embryo, 9-12 dpc

text: DH10B (phage-resistant)

text: brain

text: Mus musculus

text: NotI

text: EcoRI

text: pituitary gland

text: Soares NMBP1

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: 1st strand cDNA was primed with a NotI - oligo(dT) primer 5'-AACTGGAAGAATTCGCGGCCGCGTATCCATGATTTTTTTTTTTTTTTTTTT-3'; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' AND 5'-CCTCGTGCCG-3'(Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library went through one round of normalization, and was constructed in the laboratory of M. Bento Soares (University of Iowa).

text: adult

text: DH10B (phage-resistant)

text: brain

text: Mus musculus

text: NotI

text: EcoRI

text: pituitary gland

text: Soares NMBPA

text: phagemid (ampicillin resistant)


text: USDA, ARS, Animal and Natural Resources Institute

text: live

text: Generic

text: epithelium

characteristic: cell type

text: This mammary-derived cDNA library was created as part of a collaborative project between the ARS Gene Evaluation and Mapping Laboratory and the EMBRAPA Dairy Cattle Research Center under the sponsorship of USDA, ARS/EMBRAPA-LABEX program in animal genomics. Mammary RNA samples from AM2, AM4, and AM5 extracted on 3/27/02, , RT with Superscript II at 37 deg C annealing temperature, PCR with 16-mers

text: involuted

text: mammary

text: Bos indicus

text: HincII

text: HincII

text: female

characteristic: sex

text: Brazilian Dairy Gir

text: parenchyma

text: BARC-EMBRAPA 319BOV

text: plasmid

text: pUC 118


characteristic: External Id

text: live

text: Generic

text: multiple

text: This mammary-derived cDNA library was created as part of a collaborative project between the ARS Gene Evaluation and Mapping Laboratory and the EMBRAPA Dairy Cattle Research Center under the sponsorship of USDA, ARS/EMBRAPA-LABEX program in animal genomics.RNA samples from multiple animals(AM1, AM2, AM3, and AM4), RT with Superscript II at 37 deg C annealing temperature, PCR with 16-mers.

text: involuted

text: mammary

text: Bos indicus

text: HincII

text: HincII

text: female

text: Brazilian Dairy Gir

characteristic: strain

text: teat cistern and parenchyma

text: BARC-EMBRAPA 321BOV

text: plasmid

text: pUC 118


characteristic: INSDC center name

text: live

text: Generic

text: epithelium

text: This mammary-derived cDNA library was created as part of a collaborative project between the ARS Gene Evaluation and Mapping Laboratory and the EMBRAPA Dairy Cattle Research Center under the sponsorship of USDA, ARS/EMBRAPA-LABEX program in animal genomics. RNA samples from AM2, AM4, and AM5 extracted on 3/27/02, RT with Superscript II at 37 deg C annealing temperature, PCR with 16-mers.

text: involuted

text: mammary

text: Bos indicus

text: HincII

text: HincII

text: female

text: Brazilian Dairy Gir

text: parenchyma

text: BARC-EMBRAPA 322BOV

text: plasmid

text: pUC 118

characteristic: vector


characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: epithelium

text: This mammary-derived cDNA library was created as part of a collaborative project between the ARS Gene Evaluation and Mapping Laboratory and the EMBRAPA Dairy Cattle Research Center under the sponsorship of USDA, ARS/EMBRAPA-LABEX program in animal genomics. RNA sample from cow AM1, samples 2, 4, and 5 extracted on 3/27/02, RT with Superscript II at 37 deg C annealing temperature, PCR with 16-mers.

text: involuted

text: mammary

text: Bos indicus

text: HincII

text: HincII

text: female

text: Brazilian Dairy Gir

text: parenchyma

text: BARC-EMBRAPA 323BOV

text: plasmid

text: pUC 118


text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: epithelium

text: This mammary-derived cDNA library was created as part of a collaborative project between the ARS Gene Evaluation and Mapping Laboratory and the EMBRAPA Dairy Cattle Research Center under the sponsorship of USDA, ARS/EMBRAPA-LABEX program in animal genomics.RNA sample from cow AM1, samples 2, 4, and 5 extracted on 3/27/02, RT with Superscript II at 37 deg C annealing temperature, PCR with 16-mers.

text: involuted

text: mammary

text: Bos indicus

text: HincII

text: HincII

text: female

text: Brazilian Dairy Gir

text: parenchyma

text: BARC-EMBRAPA 324BOV

text: plasmid

text: pUC 118


text: live

text: Generic

characteristic: NCBI submission package

text: epithelium

characteristic: description

text: involuted

text: mammary

text: Bos indicus

text: HincII

text: HincII

text: female

text: Brazilian Dairy Gir

text: parenchyma

text: BARC-EMBRAPA 326BOV

text: plasmid

text: pUC 118


text: live

text: Generic

text: epithelium

text: This mammary-derived cDNA library was created as part of a collaborative project between the ARS Gene Evaluation and Mapping Laboratory and the EMBRAPA Dairy Cattle Research Center under the sponsorship of USDA, ARS/EMBRAPA-LABEX program in animal genomics. RNA sample from cow AM1, samples 7 and 9 extracted on 3/6/02, RT with Superscript II at 37 deg C annealing temperature, PCR with 16-mers.

text: involuted

characteristic: dev-stage

characteristic: lab-host

text: mammary

text: Bos indicus

text: HincII

text: HincII

text: female

text: Brazilian Dairy Gir

text: parenchyma

text: BARC-EMBRAPA 327BOV

text: plasmid

text: pUC 118


text: live

text: Generic

text: epithelium

text: involuted

text: mammary

text: Bos indicus

characteristic: organism

text: HincII

text: HincII

text: female

text: Brazilian Dairy Gir

text: parenchyma

text: BARC-EMBRAPA 328BOV

characteristic: title

text: plasmid

text: pUC 118


text: live

text: Generic

text: multiple

text: This mammary-derived cDNA library was created as part of a collaborative project between the ARS Gene Evaluation and Mapping Laboratory and the EMBRAPA Dairy Cattle Research Center under the sponsorship of USDA, ARS/EMBRAPA-LABEX program in animal genomics. RNA extracted on 6/8/02, RT with Superscript II at 37 deg C annealing temperature. Cow AM1.

text: involuted

text: mammary

characteristic: organ

text: Bos indicus

text: HincII

text: HincII

text: female

text: Brazilian Dairy Gir

text: teat cistern and alveoli

characteristic: tissue-type

text: BARC-EMBRAPA 338BOV

text: plasmid

text: pUC 118


text: live

text: Generic

text: epithelium

characteristic: cell type

text: involuted

text: mammary

text: Bos indicus

text: HincII

text: HincII

text: female

characteristic: sex

text: Brazilian Dairy Gir

text: parenchyma

text: BARC-EMBRAPA 356BOV

text: plasmid

text: pUC 118


characteristic: External Id

text: live

text: Generic

text: epithelium

text: involuted

text: mammary

text: Bos indicus

text: HincII

text: HincII

text: female

text: Brazilian Dairy Gir

characteristic: strain

text: parenchyma

text: BARC-EMBRAPA 355BOV

text: plasmid

text: pUC 118


characteristic: INSDC center name

text: live

text: Generic

text: multiple

text: involuted

text: mammary

text: Bos indicus

text: SmaI

text: SmaI

text: female

text: Brazilian Dairy Gir

text: teat cistern and alveoli

text: BARC-EMBRAPA 339BOV

text: plasmid

text: pUC 19

characteristic: vector


characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: epithelium

text: involuted

text: mammary

text: Bos indicus

text: HincII

text: HincII

text: female

text: Brazilian Dairy Gir

text: parenchyma

text: BARC-EMBRAPA 352BOV

text: plasmid

text: pUC 118


text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: epithelium

text: involuted

text: mammary

text: Bos indicus

text: HincII

text: HincII

text: female

text: Brazilian Dairy Gir

text: parenchyma

text: BARC-EMBRAPA 351BOV

text: plasmid

text: pUC 118


text: live

text: Generic

characteristic: NCBI submission package

text: epithelium

characteristic: description

text: involuted

text: mammary

text: Bos indicus

text: HincII

text: HincII

text: female

text: Brazilian Dairy Gir

text: parenchyma

text: BARC-EMBRAPA 353BOV

text: plasmid

text: pUC 118


text: live

text: Generic

text: epithelium

text: involuted

characteristic: dev-stage

characteristic: lab-host

text: mammary

text: Bos indicus

text: HincII

text: HincII

text: female

text: Brazilian Dairy Gir

text: parenchyma

text: BARC-EMBRAPA 354BOV

text: plasmid

text: pUC 118


text: Universidade de Sao Paulo

text: live

text: Generic

text: Reticulocytes

text: insect vector: Anopheles darlingi ; Speranca et al. 2001. Exp. Parasitol., 97:119-128.

text: Assexual Blood Stages

text: Homo sapiens

text: Plasmodium vivax

characteristic: organism

text: Blood

text: PvBEcDNA

characteristic: title

text: Lambda-Express (Stratagene)


text: College of Agriculture and Biological Sciences

text: live

text: Generic

characteristic: cultivar

text: The ESTs were generated from substractive library with the Suppression Subtractive Hybridization (SSH) between infected and uninfected roots total RNA ( root total RNA in different intervals 1, 3, 7, 12, 24, 48, 72 hours post-inoculation were put into together as the same ratio) of G.babardense variety 7124. The infected pathogens was a kind of highly aggressive defoliating V. dahliae fungus VJ37 from Hebei province in China.

text: different intervals after root infected with Verticillium dahliae

text: root

characteristic: organ

text: Gossypium barbadense

text: male and female

text: root

characteristic: tissue-type

text: Gossypium barbadense SSH short sequences

text: plasmid

text: pGEM-T vector


characteristic: External Id

text: Nanjing Agricultural University

text: live

text: Generic

text: ESTs derived from mRNA of growing follicles by DD-RTPCPR

text: Anas platyrhynchos

text: female

characteristic: sex

text: Shaoxing duck

text: Ovary follicles

text: The library of duck follicles

text: plasmid

text: pMD 18-T Vector


text: Penn State University

characteristic: INSDC center name

text: live

text: Generic

text: Columbia

text: Clontech's PCR Suppression Subtractive Hybridization was used. Tester = plants sprayed with 50mM methyl jasmonate, and tissue harvested 1,8, 24 and 48h after treatment; driver = plants sprayed with water and tissue harvested 1,8, 24 and 48h. Cloned into Advantage PCR cloning vector (Clontech).

text: Arabidopsis thaliana

text: Leaf

text: Methyl Jasmonate Treated Arabidopsis Forward-Subtracted Library

text: Advantage PCR cloning vector (ClonTech)


text: Penn State University

text: live

text: Generic

text: Columbia

text: Clontech's PCR Suppression Subtractive Hybridization was used. Tester = ovaries collected within 24 hrs of pollination from wild type plants; driver = a male-sterile mutant. Cloned into Advantage PCR cloning vector (Clontech).

text: Arabidopsis thaliana

text: Ovule

text: Early Ovule Development Forward Subtracted Library

text: Advantage PCR cloning vector (ClonTech)

characteristic: vector


text: Penn State University

characteristic: INSDC first public

text: live

text: Generic

text: Columbia

text: Clontech's PCR Suppression Subtractive Hybridization was used. Tester = plants sprayed with spores of Peronospora parasitica strain Ahco, and tissue harvested 1,3 and 5 days after infection ; driver = plants sprayed with water and tissue harvested 1,3 and 5 days. Cloned into Advantage PCR cloning vector (Clontech).

text: Arabidopsis thaliana

text: Leaf

text: Virulent Peronospora parasitica Infected Arabidopsis Forward-Subtracted Library

text: Advantage PCR cloning vector (ClonTech)


text: Penn State University

characteristic: INSDC last update

text: live

text: Generic

text: Columbia

text: Clontech's PCR Suppression Subtractive Hybridization was used. Tester = plants sprayed with spores of Peronospora parasitica strain Emwa, and tissue harvested 12h, 1, 2 and 3 days after infection ; driver = plants sprayed with water and tissue harvested 12h, 1, 2 and 3 days. Cloned into Advantage PCR cloning vector (Clontech).

text: 3-week old plants

text: Arabidopsis thaliana

text: Leaf

text: Avirulent Peronospora parasitica Infected Forward Subtracted Library

text: Advantage PCR cloning vector (ClonTech)


text: Penn State University

text: live

characteristic: INSDC status

text: Generic

text: Columbia

text: Clontech's PCR Suppression Subtractive Hybridization was used. Tester = plants sprayed with water and tissue harvested 1,3 and 5 days; driver = plants sprayed with spores of Peronospora parasitica strain Ahco, and tissue harvested 1,3 and 5 days after infection. Cloned into Advantage PCR cloning vector (Clontech).

text: Arabidopsis thaliana

text: Leaf

text: Virulent Peronospora parasitica Infected Arabidopsis reverse-Subtracted Library

text: Advantage PCR cloning vector (ClonTech)


text: Penn State University

text: live

text: Generic

characteristic: NCBI submission model

text: Columbia

text: Clontech's PCR Suppression Subtractive Hybridization was used. Tester = plants sprayed with water and tissue harvested 12h, 1, 2 and 3 days.; driver = plants sprayed with spores of Peronospora parasitica strain Emwa, and tissue harvested 12h, 1, 2 and 3 days after infection Cloned into Advantage PCR cloning vector (Clontech).

text: 3-week old plants

text: Arabidopsis thaliana

text: Leaf

text: Avirulent Peronospora parasitica Infected Reverse Subtracted Library

text: Advantage PCR cloning vector (ClonTech)


text: Penn State University

text: live

text: Generic

characteristic: NCBI submission package

text: Columbia

text: Clontech's PCR Suppression Subtractive Hybridization was used. Tester = plants sprayed with water and tissue harvested 1,8, 24 and 48h; driver = plants sprayed with 50mM methyl jasmonate, and tissue harvested 1,8, 24 and 48h after treatment. Cloned into Advantage PCR cloning vector (Clontech).

text: Arabidopsis thaliana

text: Leaf

text: Methyl Jasmonate Treated Arabidopsis Reverse-Subtracted Library

text: Advantage PCR cloning vector (ClonTech)


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: 1st strand cDNA (prepared from post mortem tissue) was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCCGTTTTTTTTTTTTTTTTTT 3'], double- stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library went through one round of normalization, to Cot38. Library constructed by Bento Soares and M. Fatima Bonaldo

characteristic: description

text: adults (ages 18, 20, 48)

text: Homo sapiens

text: brain, pineal gland

text: Soares pineal gland 3NbHPG


text: Penn State University

text: live

text: Generic

text: Columbia

text: Clontech's PCR Suppression Subtractive Hybridization was used. Tester = plants exposed to 0.4 ul/L of ozone for 6 hours and tissue harvested 3, 6, 9,12 and 24h after treatment ; driver = plants exposed to clean air and tissue harvested 3, 6, 9 12 and 24 h. Cloned into Advantage PCR cloning vector (Clontech).

text: 3-week old plants

characteristic: dev-stage

text: Arabidopsis thaliana

text: Leaf

text: Arabidopsis Acute Ozone pooled time-points Forward-Subtracted Library

text: Advantage PCR cloning vector (ClonTech)


text: Cancer Institute (hospital) Peking Union Medical College (PUMC) & Chinese Academy of Medical Sciences (CAMS)

text: live

text: Generic

text: lymph node metastasis tumor cells

characteristic: cell type

text: country: USA; screen drug-sentitive genes differentially expressed in human lymph node metastasis cells by suppression subtractive hybridization

text: metastasis lymph node

characteristic: lab-host

text: Homo sapiens

text: RsaI

text: RsaI

text: male

text: lymph node

text: suppression subtractive hybridization Library

text: Plasmid

text: pGEM-T


text: Southwest Missouri State University-Mountain Grove

text: live

text: Generic

text: Norton

text: VAN-Baker-1 is a cDNA library of Norton grape young leaves (Vitis. aestivalis var. Norton). Norton grapevines were grown under normal greenhouse conditions. The cDNA synthesis and library construction was performed according to the instruction manual for pBluescript II XR cDNA library construction kit provided by Stratagene.

text: Young leaf

text: XL10-Gold E .coli

text: Vitis aestivalis

characteristic: organism

text: Xho I

text: EcoR I

text: Leaf

characteristic: title

text: Plasmid

text: pBluescript II SK (+) Phagemid


text: Nanjing Agricultural University

text: live

text: Generic

characteristic: cultivar

text: Genes differentially expressed during microspore formation and development were identified by comparing DD-RT-PCR profiles of the young anther of the CMS restoring line (Rf1Rf1), the CMS line, the maintainer and F1 in Gossypium hirsutumanther. Differentially expressed bands were excised and sequenced for functional annotation.

text: Gossypium hirsutum

text: anther

text: cotton anther cDNA(differential display PCR)


text: Faculty of Agriculture and Natural Science, University of Pretoria

text: live

text: Generic

text: mRNA/cDNA

text: 24 h post inoculation

text: Pinus patula

text: stem

characteristic: tissue-type

text: Fusarium circinatum induced cDNA library from Pinus patula


characteristic: External Id

text: University of Texas at El Paso

text: live

text: Generic

text: Sequenced from products cloned into InVitrogen's pCR 2.1 vector.

text: adult

text: liver

characteristic: organ

text: Pseudopleuronectes americanus

text: Subtractive library from winter flounder liver


text: Nanjing Medical University

characteristic: INSDC center name

text: live

text: Generic

text: by use of SMART CDNA Library Construction KIT

text: four-instar larval

text: E.coli JM109

text: Culex pipiens pallens

text: Sfi 1A

text: Sfi 1B

text: female

characteristic: sex

text: insecticide - resistance strain

characteristic: strain

text: Culex pipiens pallens cDNA express library

text: phage

text: PGEM-- TEASY


text: Cold Spring Harbor Laboratory

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Date: Completed 09/01/2001. Submitted to CSHL 09/05/2001. Sample: Young emergent leaves. From New York Botanical Garden Conservatory accssion number 808/59 A (collected 03/2001). Library: Made using Stratagene's ZAP Express Vector Kit. Library was size fractionated for large inserts.

text: Young leaf

text: Cycas rumphii

text: Xho I

text: Eco RI

text: Female

text: Cycad Leaf Library (NYBG)

text: phage

text: pBK-CMV

characteristic: vector


text: University of Aberdeen

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Vibrio vulnificus

text: Vibrio vulnificus MO6-24/T


text: Nanjing Agricultural University

text: live

text: Generic

text: Genes differentially expressed during microspore formation and development were identified by comparing DD-RT-PCR profiles of the young leaf of the CMS restoring line (Rf1Rf1),the CMS line,the maintainer and F1 in Gossypium hirsutum. Differentially expressed bands were excised and sequenced for functional annotation.

characteristic: description

text: Gossypium hirsutum

text: leaf

text: cotton leaf cDNA (differential display PCR)


text: Washington University School of Medicine

text: live

text: Generic

text: The library was constructed by Claire Murphy and Dr. James McCarter at Washington University, St. Louis. The cDNA was made by using Dynabead oligo-dT priming (Dynal). PCR based library using a modified protocol from the SMART PCR cDNA Synthesis Kit from Clontech. Directionally cloned into the UDG sites of pAMP1. Adult nematodes were collected from infected dogs by Dr. Prema Arasu of North Carolina State University, Raleigh, NC (Prema_Arasu@ncsu.edu) and RNA was prepared by Merry McLaird of Divergence Inc, St. Louis, MO (mclaird@divergence.com).

text: adult

characteristic: dev-stage

characteristic: lab-host

text: Trichuris vulpis

text: NotI

text: SalI

text: Trichuris vulpis pAMP1 v1

text: plasmid (ampicillin resistant)

text: pAMP1 (Gibco)


text: Korea Research Institute of Bioscience & Biotechnology

text: live

text: Generic

text: The poly (A)+ RNA was dephosphorylated with bacterial alkaline phosphatase (BAP) and then decapped with tabacco acid pyrophosphatase (TAP). The decapped intact mRNA was ligated with DNA-RNA linker including EcoRI site by treatment of T4 RNA ligase and the first strand cDNA was synthesized from oligo dT-selected mRNA by priming with dT-tailed vector. The dT-tailed vector was adjusted to have about 60nt. The cDNA vector was circularized with E. coli DNA ligase after digestion of EcoRI which site is also included in vector. An RNA strand converted to a DNA strand by Okayama-Berg method. The obtained cDNA vectors were used for transformation of competent cells E. coli Top10F' by electroporation method. The cDNA libraries constructed by this method are full-length enriched cDNA library.

text: Brain

text: Homo sapiens

characteristic: organism

text: EcoRI

text: NotI

text: M

characteristic: title

text: Plasmid


text: live

text: Generic

text: The poly (A)+ RNA was dephosphorylated with bacterial alkaline phosphatase (BAP) and then decapped with tabacco acid pyrophosphatase (TAP). The decapped intact mRNA was ligated with DNA-RNA linker including EcoRI site by treatment of T4 RNA ligase and the first strand cDNA was synthesized from oligo dT-selected mRNA by priming with dT-tailed vector. The dT-tailed vector was adjusted to have about 60nt. The cDNA vector was circularized with E. coli DNA ligase after digestion of EcoRI which site is also included in vector. An RNA strand converted to a DNA strand by Okayama-Berg method. The obtained cDNA vectors were used for transformation of competent cells E. coli Top10F' by electroporation method. The cDNA libraries constructed by this method are full-length enriched cDNA library. After analyzing and sequencing about 2,000 - 3,000 colonies in original cDNA library, the abundant cDNAs were selected and amplified by PCR reaction using vector region primer including T7 promotor as 5' primer and N(dT)14 as 3' primer. The PCR products were used as template for synthesis of biotinylated single stranded RNA by in vitro transcription reaction. The synthesized RNA probes were hybridized with antisense single stranded cDNAs prepared from original liberary and incubated with avidin-gel. After removing DNA-RNA hybrids by centrifuge, the subtracted cDNA libraries were constructed by transformaion of the remaining DNA into competent cells E. coli Top10F' with electroporation method.

text: Brain

text: Homo sapiens

text: EcoRI

text: NotI

text: M

text: Plasmid


text: live

text: Generic

text: Epithelial

characteristic: cell type

characteristic: cell-line

text: Cervix

text: Homo sapiens

text: EcoRI

text: NotI

text: F

text: Uterine

characteristic: tissue-type

text: Plasmid


characteristic: External Id

characteristic: INSDC center name

text: live

text: Generic

text: Epithelial

text: Cervix

characteristic: organ

text: Homo sapiens

text: EcoRI

text: NotI

text: F

text: Uterine

text: Plasmid


characteristic: INSDC first public

text: live

text: Generic

text: Cho-CK

text: The library was contributed by the Soares laboratory and it was constructed as described by Bonaldo, M.F., Lennon, G. and Soares, M.B. (1996), Genome Research 6(9): 791-806. RNA was prepared from harvested cell culture.

text: Liver

text: Homo sapiens

text: EcoRI

text: NotI

text: M

characteristic: sex

text: Plasmid


characteristic: INSDC last update

text: live

text: Generic

text: Polygonal

text: Liver

text: Homo sapiens

text: EcoRI

text: NotI

text: M

text: Liver

text: Plasmid

characteristic: vector


text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Polygonal

text: Liver

text: Homo sapiens

text: EcoRI

text: NotI

text: M

text: Liver

text: Plasmid


text: live

text: Generic

characteristic: NCBI submission package

text: J-SHC

characteristic: description

text: Liver

text: Homo sapiens

text: EcoRI

text: NotI

text: M

text: Plasmid


text: live

text: Generic

text: J-SHC

characteristic: lab-host

text: Liver

text: Homo sapiens

text: EcoRI

text: NotI

text: M

text: Plasmid


text: live

text: Generic

text: Epithelial

text: Liver

text: Homo sapiens

characteristic: organism

text: EcoRI

text: NotI

text: F

text: Liver

text: Plasmid


text: live

text: Generic

text: Epithelial

text: Liver

text: Homo sapiens

text: EcoRI

text: NotI

text: F

text: Liver

characteristic: title

text: Plasmid


text: live

text: Generic

text: Choi-CK

text: Liver

text: Homo sapiens

text: EcoRI

text: NotI

text: M

text: Plasmid


text: live

text: Generic

text: Liver

text: Homo sapiens

text: EcoRI

text: NotI

text: M

text: Plasmid


text: live

text: Generic

text: Liver

text: Homo sapiens

text: EcoRI

text: NotI

text: M

text: Plasmid


text: live

text: Generic

text: Liver

text: Homo sapiens

text: EcoRI

text: NotI

text: Plasmid


text: live

text: Generic

text: Liver

text: Homo sapiens

text: EcoRI

text: NotI

text: F

text: Plasmid


text: live

text: Generic

text: Liver

text: Homo sapiens

text: EcoRI

text: NotI

text: F

text: Plasmid


text: live

text: Generic

text: Liver

text: Homo sapiens

text: EcoRI

text: NotI

text: M

text: Plasmid

text: pCNS


text: live

text: Generic

text: The poly (A)+ RNA was dephosphorylated with bacterial alkaline phosphatase (BAP) and then decapped with tabacco acid pyrophosphatase (TAP). The decapped intact mRNA was ligated with DNA-RNA linker including SfiI site by treatment of T4 RNA ligase and the first strand cDNA was synthesized with Superscript II using SfiI oligo-dT primer. After first strand synthesis, RNA was degraded by NaOH treatment and cDNA was amplified by PCR reaction. The PCR products were digested with SfiI and cloned into DraIII-digested pME18S-FL3 vector. The obtained cDNA vectors were used for transformation of competent cells E. coli Top10F' by electroporation method. The cDNA libraries constructed by this method are full-length enriched cDNA library.

text: Liver

text: Homo sapiens

text: XhoI

text: XhoI

text: M

text: Plasmid


text: live

text: Generic

text: Cho-CK

text: Liver

text: Homo sapiens

text: EcoRI

text: NotI

text: M

text: Plasmid


text: live

text: Generic

text: Cho-CK

text: Liver

text: Homo sapiens

text: EcoRI

text: NotI

text: M

text: Plasmid


text: live

text: Generic

text: Liver

text: Homo sapiens

text: EcoRI

text: NotI

text: M

text: Plasmid


text: live

text: Generic

text: Liver

text: Homo sapiens

text: EcoRI

text: NotI

text: M

text: Plasmid


text: live

text: Generic

text: SCK

text: Liver

text: Homo sapiens

text: EcoRI

text: NotI

text: M

text: Plasmid


text: live

text: Generic

text: Liver

text: Homo sapiens

text: EcoRI

text: NotI

text: M

text: Plasmid


text: live

text: Generic

text: Stomach

text: Homo sapiens

text: EcoRI

text: NotI

text: F

text: Plasmid


text: live

text: Generic

text: Thymus

text: Homo sapiens

text: EcoRI

text: NotI

text: M

text: Plasmid


text: NONG WOO BIO CO.,LTD.

text: live

text: Generic

characteristic: cultivar

text: PMMV-induced suppression subtractive cDNA library of Hot pepper. The subtraction was performed using the PCR-select cDNA subtraction kit (Clontech, Palo Alto, CA) according to the manufacturer's instructions. For a tester, cDNA from HR-developing pepper leaves sampled after 3 days inoculation was synthesized using AMV reverse transcriptase while, for a driver, cDNA from noninoculated pepper leaves was synthesized. After the subtractive hybridization, the subtracted cDNAs are selectively amplified by using nested PCR primers to enrich differentially expressed sequences.

text: 7-8 weeks-old leaves

characteristic: dev-stage

text: Capsicum chinense

text: EcoRI

text: hermaphrodite

text: leaf

text: plasmid

text: pGEM-T easy


text: NONG WOO BIO CO.,LTD.

text: live

text: Generic

text: SIRO, SBV

text: SIRO red - SBV red suppression subtractive cDNA library of Hot pepper. The subtraction was performed using the PCR-select cDNA subtraction kit (Clontech, Palo Alto, CA) according to the manufacturer's instructions. For a tester, cDNA from HR-developing pepper leaves sampled after 3 days inoculation was synthesized using AMV reverse transcriptase while, for a driver, cDNA from noninoculated pepper leaves was synthesized. After the subtractive hybridization, the subtracted cDNAs are selectively amplified by using nested PCR primers to enrich differentially expressed sequences.

text: mature red

text: Capsicum annuum

text: EcoRI

text: hermaphrodite

text: Fruit

characteristic: tissue-type

text: plasmid

text: pGEM-T easy


characteristic: External Id

text: NONG WOO BIO CO.,LTD.

text: live

text: Generic

text: SIRO

text: SIRO red - SIRO green/red suppression subtractive cDNA library of Hot pepper. The subtraction was performed using the PCR-select cDNA subtraction kit (Clontech, Palo Alto, CA) according to the manufacturer's instructions. For a tester, cDNA from HR-developing pepper leaves sampled after 3 days inoculation was synthesized using AMV reverse transcriptase while, for a driver, cDNA from noninoculated pepper leaves was synthesized. After the subtractive hybridization, the subtracted cDNAs are selectively amplified by using nested PCR primers to enrich differentially expressed sequences.

text: mature red, green/red

text: Capsicum annuum

text: EcoRI

text: hermaphrodite

text: Fruit

text: plasmid

text: pGEM-T easy


text: Texas A&M University

characteristic: INSDC center name

text: live

text: Generic

text: sequence 5' of the insert (5'-NNN...NNNinsert) GCGAATTGGAGCTCCACCGCGGTGGCGGCCGCGGCTCGAG. Sequence 3' of the inserts (AAGAATTCGATATCAAGCTTATCGATACCGTCGACCTCGAG. non-normalized library, sequenced 3' with M13R primer.

text: liver

text: Bos taurus

text: NotI

text: EcoRI

text: liver

text: CSEQFXL02 liver

text: Plasmid

text: pBluescript SK+


text: Texas A&M University

text: live

text: Generic

text: sequence 5' of the insert (5'-NNN...NNNinsert) GCGAATTGGAGCTCCACCGCGGTGGCGGCCGCGGCTCGAG. Sequence 3' of the inserts (AAGAATTCGATATCAAGCTTATCGATACCGTCGACCTCGAG. normalized Rd 2 library, sequenced 3' with M13R primer.

text: liver

characteristic: organ

text: Bos taurus

text: NotI

text: EcoRI

text: liver

text: CSEQFXN34 liver

text: Plasmid

text: pBluescript SK+


text: Texas A&M University

characteristic: INSDC first public

text: live

text: Generic

text: sequence 5' of the insert (5'-NNN...NNNinsert) GCGAATTGGAGCTCCACCGCGGTGGCGGCCGCGGCTCGAG. Sequence 3' of the inserts (AAGAATTCGATATCAAGCTTATCGATACCGTCGACCTCGAG. normalized Rd 1 library, sequenced 3' with M13R primer.

text: liver

text: Bos taurus

text: NotI

text: EcoRI

text: liver

text: CSEQFXN33 liver

text: Plasmid

text: pBluescript SK+


text: Texas A&M University

text: live

text: Generic

text: kidney

text: Bos taurus

text: NotI

text: EcoRI

text: kidney

text: CSEQFXL03 kidney

text: Plasmid

text: pBluescript SK+


text: Texas A&M University

text: live

text: Generic

text: kidney

text: Bos taurus

text: NotI

text: EcoRI

text: kidney

text: CSEQFXN35 kidney

text: Plasmid

text: pBluescript SK+


text: Texas A&M University

text: live

text: Generic

text: kidney

text: Bos taurus

text: NotI

text: EcoRI

text: kidney

text: CSEQFXN36 kidney

text: Plasmid

text: pBluescript SK+


text: Texas A&M University

text: live

text: Generic

text: ovary

text: Bos taurus

text: NotI

text: EcoRI

text: ovary

text: CSEQFXN18 ovary

text: Plasmid

text: pBluescript SK+


text: Texas A&M University

text: live

text: Generic

text: ovary

text: Bos taurus

text: NotI

text: EcoRI

text: ovary

text: CSEQFXL21 ovary

text: Plasmid

text: pBluescript SK+


text: Texas A&M University

text: live

text: Generic

text: ovary

text: Bos taurus

text: NotI

text: EcoRI

text: ovary

text: CSEQFXN19 ovary

text: Plasmid

text: pBluescript SK+


text: Texas A&M University

text: live

text: Generic

text: testes

text: Bos taurus

text: NotI

text: EcoRI

text: testes

text: CSEQFXL41 testes

text: Plasmid

text: pBluescript SK+


text: Texas A&M University

text: live

text: Generic

text: testes

text: Bos taurus

text: NotI

text: EcoRI

text: testes

text: CSEQFXN41 testes

text: Plasmid

text: pBluescript SK+


text: Texas A&M University

text: live

text: Generic

text: testes

text: Bos taurus

text: NotI

text: EcoRI

text: testes

text: CSEQFXN20 testes

text: Plasmid

text: pBluescript SK+


text: Texas A&M University

text: live

text: Generic

text: testes

text: Bos taurus

text: NotI

text: EcoRI

text: testes

text: CSEQFXN21 testes

text: Plasmid

text: pBluescript SK+


text: Texas A&M University

text: live

text: Generic

text: testes

text: Bos taurus

text: NotI

text: EcoRI

text: testes

text: CSEQFXN42 testes

text: Plasmid

text: pBluescript SK+


text: Texas A&M University

text: live

text: Generic

text: adrenal

text: Bos taurus

text: NotI

text: EcoRI

text: adrenal

text: CSEQFXL14 adrenal

text: Plasmid

text: pBluescript SK+


text: Texas A&M University

text: live

text: Generic

text: thymus

text: Bos taurus

text: NotI

text: EcoRI

text: thymus

text: CSEQFL16 thymus

text: Plasmid

text: pBluescript SK+


text: Texas A&M University

text: live

text: Generic

text: pituitary

text: Bos taurus

text: NotI

text: EcoRI

text: pituitary

text: CSEQFL17 pituitary

text: Plasmid

text: pBluescript SK+


text: Texas A&M University

text: live

text: Generic

text: pituitary

text: Bos taurus

text: NotI

text: EcoRI

text: pituitary

text: CSEQFXN31 pituitary

text: Plasmid

text: pBluescript SK+


text: Texas A&M University

text: live

text: Generic

text: pituitary

text: Bos taurus

text: NotI

text: EcoRI

text: pituitary

text: CSEQFXN30 pituitary

text: Plasmid

text: pBluescript SK+


text: Universite Blaise Pascal

text: live

text: Generic

text: cDNA was prepared either from healthy or infected sunflowers 15 days post inoculation with Plasmopara halstedii. To enrich the cDNA library with clones specifically from P. halstedii; the cDNA from infected plants was used as tester and the cDNA from non infected plants was used as driver in a PCR-based subtraction strategy (PCR-selectTM cDNA Subtraction kit, Clontech). After subtraction, the PCR-amplified cDNA were cloned. The P. halstedii origin of the EST was confirmed by PCR using specific primers.

text: E. coli strain JM109

text: Plasmopara halstedii

text: Race 300

characteristic: strain

text: Mycelium

text: Plasmopara halstedii SSH

text: P-Gem-Teasy


text: National Cancer Institute / NIH

characteristic: INSDC last update

text: live

text: Generic

text: ORF's were PCR-amplified (from IMAGE clones or from commercially available cDNA libraries) and cloned by the Guthrie cDNA Resource Center (www.guthrie.org/cDNA) into pcDNA3.1. For specific information on cloning sites (which vary by clone), please refer to the Guthrie website, using the Guthrie ID given in the file ftp://image.llnl.gov.image.rearrayed_plates/IRBF.preSV.data. Note: this is a NIH_MGC Library.

text: DH10B (T1-phage-resistant)

text: Homo sapiens

text: multiple

text: multiple

text: mixed

text: plasmid (ampicillin resistant)


text: live

text: Generic

text: Normalized full-length enriched library from pooled mouse embryonic limb, maxilla and mandible, day 12.5, 13.5, 14.5, and 15.5 (size selected for the 0.5-1 kb fragments) Cloned directionally, priming method: Oligo-dT. cDNA enrichment: >1k bp, Average insert size 1.6k bp. Normalization (Cot value): 7.5 kb. Priming sequence: 5'GACTAGTTCTAGATCGCGAGCGGCCGCCC(T)3' Tissue contributed by, David Rowe. Library constructed by ResGen, Invitrogen Corp.

text: DH10B (phage-resistant)

text: Mus musculus

text: EcoRV

text: NotI

text: phagemid (ampicillin resistant)

characteristic: vector


text: University of Texas at El Paso

text: live

characteristic: INSDC status

text: Generic

text: adult

text: liver

text: Fundulus heteroclitus

text: male

characteristic: sex

text: Fundulus heteroclitus adult male liver


text: Korea Forest Research Institute

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: XL1-Blue MRF' strain

text: Populus alba x Populus glandulosa

text: EcoRI

text: XhoI

text: Suspension cell

text: Poplar SC cDNA library

text: Phage

text: Uni-ZAP XR


text: Hellenic Centre for Marine Research Crete

text: live

text: Generic

text: embryonic and larvae

text: Sparus aurata

text: Embryonic and Larvae stages, Stratagene (Catalog #200451)

text: pBK-CMV phagemid vector


text: live

text: Generic

text: adult

text: liver

text: Sparus aurata

text: adult liver, Stratagene (Catalog #200451)

text: pBK-CMV phagemid vector


text: National Institute of Health

text: live

text: Generic

text: Normal melanocytes Melan A

characteristic: cell type

text: fragment of cDNA from Differential Display; Normal melanocytes Melan A (Provided by Dr. Dorothy C. Bennett. St. George's Hospital Medical School, London England)

characteristic: description

text: Mus musculus

text: Skin

text: Normal Melanocytes, Melan A


text: National Institute of Health

text: live

text: Generic

text: B16 melanoma BL6 cells

text: cDNA (RT-PCR)

text: Mus musculus

characteristic: organism

text: Skin

text: B16 melanoma BL6 cells

characteristic: title


text: Telethon Institute for Child Health Research

text: live

text: Generic

text: primary leukaemia cells

text: cDNA generated by RT-PCR from total RNA extracted from human leukaemic cells

text: Homo sapiens

text: Homo sapiens leukaemia cells RT-PCR


text: Umea Plant Science Centre

text: live

text: Generic

text: Salix viminalis

text: Leaf

text: Salix viminalis leaf Lambda TriplEx library

text: Plasmid

text: pTriplEx


text: The Pennsylvania State University

text: live

text: Generic

text: Columbia

characteristic: cultivar

text: A cDNA library enriched for genes differentially expressed during activation of systemic acquired resistance (in response to salicylic acid treatment) was constructed using CLONTECH PCR-Select cDNA Subtraction Kit (CLONTECH Laboratories, Inc.). 'Tester'= leaf tissue from plants sprayed with 1 mM salicylic acid, 'Driver' = leaf tissue from plants sprayed with water. CDNA fragments were cloned in pGEM T-vector

text: Four-week-old plants

characteristic: dev-stage

text: Arabidopsis thaliana

text: Rosette leaf

text: Arabidopsis Salicylic Acid Subtracted Library

text: pGEM T-vector


characteristic: External Id

text: The Pennsylvania State University

text: live

text: Generic

text: Columbia

text: A cDNA library enriched for Arabidopsis genes differentially expressed during activation of hypersensitive resistance (in response to infection by P. syringae pv. tomato DC3000 expressing avrRpm1) was constructed using CLONTECH PCR-Select cDNA Subtraction Kit (CLONTECH Laboratories, Inc.). 'Tester'= leaf tissue from plants infiltrated with 5 x 107 cfu/mL of Pst DC3000 (avrRpm1), 'Driver' = leaf tissue from plants infiltrated with 10 mM MgCl2. cDNA fragments were cloned in PCR 2.1 TOPO vector

text: Four-week-old plants

text: Arabidopsis thaliana

text: Rosette leaf

characteristic: tissue-type

text: Arabidopsis avirulent Pseudomonas syringae subtracted library

text: PCR 2.1 TOPO vector


text: The Pennsylvania State University

characteristic: INSDC center name

text: live

text: Generic

text: Columbia

text: A cDNA library enriched for Arabidopsis genes differentially expressed during disease (in response to infection by P. syringae pv. tomato DC3000) was constructed using CLONTECH PCR-Select cDNA Subtraction Kit (CLONTECH Laboratories, Inc.). 'Tester'= leaf tissue from plants infiltrated with 5 x 107 cfu/mL of Pst DC3000, 'Driver' = leaf tissue from plants infiltrated with 10 mM MgCl2. cDNA fragments were cloned in PCR 2.1 TOPO vector

text: Four-week-old plants

text: Arabidopsis thaliana

text: Rosette leaf

text: Arabidopsis virulent Pseudomonas syringae subtracted library

text: PCR 2.1 TOPO vector


text: Rajiv Gandhi Centre For Biotechnology

characteristic: INSDC first public

text: live

text: Generic

text: Piper longum

text: female

text: developing spikes

text: Piper longum female developing spikes


text: Rajiv Gandhi Centre For Biotechnology

text: live

text: Generic

text: Piper longum

text: male

text: developing spikes

text: Piper longum male developing spikes


text: Indian Institute of Chemical Biology

text: live

text: Generic

text: intestinal epithelial cell

characteristic: cell-line

text: cDNA/mRNA sequences obtained during studies to identify differentially expressed genes in the human intestinal epithelial cell line Int407 following Vibrio cholerae infection.

text: Homo sapiens

text: Human intestinal epithelial cell line Int407


text: National University of Singapore, Faculty of Medicine

characteristic: INSDC last update

text: live

text: Generic

text: spontaneously transformed umbilical vein endothelial cell

text: oligo-dT anchored reverse transcription of mRNA

text: Homo sapiens

text: Male

text: ECV304 human umbilical vein endothelial cell line cDNA library


text: University of Chongqing Medical Sciences

text: live

text: Generic

text: subtracted library was generated through subtractive hybridization using CLONTECH PCR-Select cDNA subtraction Kit (Catalog#:K1804-1) Library is oligo-dt primed and directionally cloned using PUC57-T vector

text: Homo sapiens

text: Human PBMCs subtractive hybridization

characteristic: vector


text: National Cancer Institute / NIH

text: live

characteristic: INSDC status

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.0-4.0 kb. Tissue pooled from pre-pubertal, post pubertal sn menopausal monkeys. Constructed by Clontech. Note: this is a NICHD Library.

text: DH10B (phage-resistant)

characteristic: lab-host

text: ovary

characteristic: organ

text: Macaca mulatta

text: Sfi I

text: Sfi I

text: Ovary

text: phagemid (ampicillin resistant)

text: pDNR-LIB


text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Non-normalized full-length enriched library 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 2.05 kb (range 1.0-4.0 kb). 15/15 colonies contained inserts by PCR.This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA).Corp..

text: DH10B (phage-resistant)

text: brain

text: Mus musculus

text: SfiI (ggccattatggcc)

text: SfiI (ggccgcctcggcc)

text: plasmid (chloramphenicol resistant, grown at 30C)

text: pDNR-LIB


text: live

text: Generic

text: Cloned unidirectionally from microquantity amounts of mRNA from normal endometrial tissue (late proliferative phase, cycle day 13). Average insert size 1.9 kb. Library constructed by ResGen (Invitrogen Corporation).

characteristic: description

text: DH10B (T1-resistant)

text: uterus

text: Homo sapiens

text: NotI

text: EcoRV

text: female

characteristic: sex

text: normal endometrium, late proliferative phase, cycle day 13

text: plasmid (ampicillin resistant)

text: pCMV-SPORT6.1.ccdb (ResGen, Invitrogen Corporation)


text: live

text: Generic

text: Cloned unidirectionally from microquantity amounts of mRNA from normal endometrial tissue (mid-secretory phase, cycle day 23). Average insert size 1.6 kb. Library constructed by ResGen (Invitrogen Corporation).

text: DH10B (T1-resistant)

text: uterus

text: Homo sapiens

characteristic: organism

text: NotI

text: EcoRV

text: female

text: normal endometrium, mid-secretory phase, cycle day 23

characteristic: title

text: plasmid (ampicillin resistant)


text: live

text: Generic

text: cDNA primed using oligo-dT primer, directionally cloned into UDG sites of pAMP1. Size selected for insert sizes ranging from 0.2-2.0 kb. Normalized to Cot5. Primary library, non-amplified. Library constructed by M. Lovett. For more information on this library, please contact R. Tidwell (Washington University) or visit the COGENE website at http://hg.wustl.edu/COGENE/.

text: embryo, 6 weeks postconception

characteristic: dev-stage

text: Homo sapiens

text: mandible, pooled

text: COGENE 6E MAN

text: plasmid (ampicillin resistant)


characteristic: External Id

text: live

text: Generic

text: leukemia cells

characteristic: cell type

text: Homo sapiens

text: Human Leukemia cells subtracted library


text: Washington University School of Medicine

characteristic: INSDC center name

text: live

text: Generic

text: PCR products were directly cloned into TA cloning vector (pre-cut vector pCR2.1-TOPO was purchased from Invitrogen). Average insert size is 300bp. The insert also has same adaptor sequence on both end. The sequence of the adaptor is: 5'-ATACGTGCAGGCTGGTTACATCTG -3'

text: Homo sapiens

text: Brain, encephalitis T01-0554

characteristic: tissue-type

text: Plasmid


characteristic: INSDC first public

text: live

text: Generic

text: Homo sapiens

text: Brain, encephalitis T99-590

text: Plasmid


characteristic: INSDC last update

text: live

text: Generic

text: Homo sapiens

text: Brain, encephalitis T00-204

text: Plasmid


text: live

text: Generic

text: Homo sapiens

text: Brain, encephalitis T01-1107

text: Plasmid

characteristic: vector


text: live

characteristic: INSDC status

text: Generic

text: Homo sapiens

text: Brain, encephalitis T02-0037

text: Plasmid


text: live

text: Generic

characteristic: lab-host

text: Homo sapiens

text: Brain, encephalitis T98-1678

text: Plasmid


text: live

text: Generic

text: Homo sapiens

text: Spleen

text: Plasmid


text: live

text: Generic

text: Homo sapiens

text: Heart

text: Plasmid


text: live

text: Generic

text: Mus musculus

text: Spleen

text: Plasmid


text: Instituto Nacional de Enfermedades Infecciosas

text: live

text: Generic

text: Messenger RNA was extracted from E. granulosus protoscoleces from a single ovine hydatid cyst. cDNA fragments were obtained using a directional random priming strategy, and were cloned into PST vector containing a signalless placental alkaline phosphatase gene. Clones with a functional signal peptide were selected by alkaline phosphatase activity testing after Cos7 cells transfection.

text: Echinococcus granulosus

text: sheep strain

characteristic: strain

text: Echinococcus granulosus protoscoleces signal sequence trap (SST) Library


text: Graduate School of Bioagricultural Sciences, Nagoya University

text: live

text: Generic

characteristic: NCBI submission model

text: Apis mellifera

text: Apis mellifera library (Aoki M)


text: Dept of AFNS, University of Alberta

text: live

text: Generic

characteristic: NCBI submission package

text: Epithelial

text: Young adult

text: Abomasum

characteristic: organ

text: Bos taurus

text: EcoR I

text: Xho I

text: Smooth muscle

text: Bos taurus Abomasum #1 library

text: Phage


text: University of Algarve Centre of Marine Sciences

text: live

text: Generic

text: A cDNA library constructed using the UNI-ZAP XR cloning kit (Stratagene, La Jolla, CA) was arrayed in 96 well plates. In the library utilised double stranded cDNA is ligated into UNI-ZAP XR/EcoRI/XhoI/CIAP and packaged into phage. The library has a total of 1 x 106 primary recombinants with an average insert size of 1000bp. The strategy used for plating the library was to first carry out a mass excision of the vector pBluescript from the phage and then transform E. coli (XL1-Blue MRF').

characteristic: description

text: Pituitary

text: Sparus aurata

text: EcoR I

text: Xho I

text: hermaphrodite

characteristic: sex

text: Pituitary Library

text: phagemid

text: pBluescript II SK (+/-)


text: Texas A&M University

text: live

text: Generic

text: The subtracted PCR products were cloned into Pinpoint Xa-1 T-vector after suppression subtractive hybridization with long wing morph wasps as 'tester' and short wing morph wasp as 'driver'.

text: Melittobia digitata

characteristic: organism

text: Melittobia digitata subtracted cDNA library

characteristic: title

text: Pinpoint Xa-1 T-vector


characteristic: External Id

text: Institute of Plant Science

text: live

text: Generic

text: Eight-day seedlings

characteristic: dev-stage

text: Oryza sativa

text: root tips

text: Water-deficit induced fragments in rice seminal root tips

text: PUCm-T


text: Helix Research Institute

characteristic: INSDC center name

text: live

text: Generic

text: mesenchymal cells

characteristic: cell type

text: Homo sapiens


text: Helix Research Institute

characteristic: INSDC first public

text: live

text: Generic

text: chondrocytes

text: Homo sapiens


text: Helix Research Institute

characteristic: INSDC last update

text: live

text: Generic

text: mesenchymal stem cells

text: Homo sapiens


text: Helix Research Institute

text: live

text: Generic

text: embryonal carcinoma

text: mRNA from uninduced embryonal carcinoma

text: Homo sapiens

characteristic: vector


text: Helix Research Institute

text: live

characteristic: INSDC status

text: Generic

text: embryonal carcinoma

text: mRNA from embryonal carcinoma after retinoic acid (RA) induction

text: Homo sapiens


text: Helix Research Institute

text: live

text: Generic

text: mesenchymal stem cells

text: mRNA from mesenchymal stem cells treated with trichostatin and 5-azacytidine

text: Homo sapiens


text: National Research Institute for Child Health and Development

text: live

text: Generic

text: adult

text: Bos taurus

text: liver

characteristic: tissue-type

text: ORCS bovine liver cDNA


text: live

text: Generic

text: adult

text: Bos taurus

text: mixture of uterus and placenta

text: ORCS bovine utero-placenta cDNA


text: College of Life Science, Wuhan University

text: live

text: Generic

text: epithelial

text: sequences cloned from human respiratory epithelial cells infected with respiratory syncytial virus by DD-PCR

text: Homo sapiens

text: lung adenocarcinoma

text: human lung adenocarcinoma


text: Institut National Polytechnique de Toulouse - Ecole National Superieure Agronomique de Toulouse

text: live

text: Generic

text: protoplast

text: Emil

characteristic: cultivar

text: 1- to 5-days old protoplast

text: Helianthus annuus

text: hypocotyl


text: UCLA School of Medicine

text: live

text: Generic

characteristic: NCBI submission model

text: brain endothelial cell genes upregulated by brain-derived peptides were selected with the suppression subtractive hybridization (SSH) method using driver cDNA produced from control brain endothelial cells not incubated with brain-derived peptides derived poly A+ RNA.

text: adult

characteristic: lab-host

text: brain

text: Bos taurus

text: brain endothelial cells

text: Bovine brain endothelial cells exposed to brain-derived peptides specific cDNA library

text: plasmid

text: pCRII (Invitrogen)


text: University of Florida, IFAS

text: live

text: Generic

characteristic: NCBI submission package

text: Valencia

text: 48 hr after application of CMNP abscission agent

text: Citrus sinensis

text: calyx abscission zone, mature fruit

text: Mature fruit abscission zone cDNA subtraction library


text: State University of New York at Buffalo

text: live

text: Generic

text: RED strain, from Bruce Christensen, U. Wisconsin; This cDNA library was constructed by David Fidock in June 1996. Brieflly, three hundred hand-dissected uninfected midguts (half from 10-12 days post blood-meal and half from 7-15 days post-emergence) were used to prepare total RNA using TriZol technique. Poly(A+) RNA was directionally cloned into the EcoR I and Xho I sites of the Lambda Zap Express vector (Stratagene).

characteristic: description

text: Half 10-12 days post blood-meal and half 7-15 days no blood-meal

text: Aedes aegypti

text: EcoR I

text: Xho I

text: RED strain

characteristic: strain

text: Hand-dissected uninfected midguts

text: Aedes aegypti (Red) midgut cDNA library

text: Phage

text: Lambda Zap Express (Stratagene)


text: The Institute of Molecular Genetics, Russian Academy of Sciences

text: live

text: Generic

text: B-lymphocytes

text: The cDNA library was resulted from the subtractive hybridization of RNA from immunoblastic lymphoma cells (tracer) and RNA from centroblastic lymphoma cells (driver)

text: adult

text: lymph node

characteristic: organ

text: Homo sapiens

characteristic: organism

text: Apa I

text: EcoR I

text: male

characteristic: sex

text: Human HIV associated B-cell immunoblastic lymphoma

text: Plasmid

text: pBluescript SK


characteristic: External Id

text: University of Toledo

text: live

text: Generic

text: mRNA was isolated from the caryopsis at early, middle and late stages of development in non-apomictic (diploid) and apomistic (tetraploid) Tripsacum dactyloides. Diffential display was carried out using Delta-Differential Diplay Kit with the T and P primers provided in the kit. Differentially diplayed bands (both up-regulated and down-regulated) were cloned in pCR2.1 and sequenced.

text: Tripsacum dactyloides

text: caryopsis

text: Tripsacum dactyloides caryopsis Differential Display

characteristic: title


text: Bielefeld University

characteristic: INSDC center name

text: live

text: Generic

text: RNA from cellculture (At7) 5 days after inoculation treated with 0.002 mM cycloheximid for 2 h in the dark. Sequence submission managed by RZPD/GABI-Primary database: http://gabi.rzpd.de. This clone is available from RZPD; contact RZPD (clone@rzpd.de) for further information.

text: tissue culture

characteristic: dev-stage

text: E. coli DH5alpha mcr

text: Arabidopsis thaliana

text: NotI primer adapter

text: SalI primer adapter

text: hypocotyl


text: Bielefeld University

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Eifel-2 (Ei-2)

text: cDNA library from Arabidopsis thaliana, accession Eifel-2; ten week old total plants grown under long-day conditions in soil, whole adult plants were treated for 24 hours with different stresses, (1) at 4M-0 C in the dark, (2), at 37 Grad C in the dark, (3) lying in the lab after removing from soil, (4) in the greenhouse after wounding leaves with a foreceps, (5) in the lab watering with a 150 mM NaCl solution, (6) at 26 M-0C in the light/UV; equal quantities of stressed plant material were pooled; library was made at the Max-Planck-Institute for Plant Breeding Research, Cologne, Germany; cloning sites SalI-NotI, primer sites and orientation: T7-SalI-CCACGCGTCCG-5prime-cDNA-polyA-CC-NotI-SP6; Note: Sequencing granted in the context of the GABI Arabidopsis Verbund I: Genetic Diversity, 'Establishment of high-efficiency SNP-based mapping tools and development of methods for genome-wide mutation detection' PI: Bernd Weisshaar Sequence submission managed by RZPD/GABI-Primary database: http://gabi.rzpd.de. This clone is available from RZPD; contact RZPD (clone@rzpd.de) for further information.

text: adult plant, mixed stresses

text: E. coli TOP10

text: Arabidopsis thaliana

text: SalI

text: whole plant


text: Bielefeld University

text: live

characteristic: INSDC status

text: Generic

text: Cape Verde Island (Cvi)

text: cDNA library from Arabidopsis thaliana, accession Cvi; six weeks old total plants grown under long-day conditions in soil, whole adult plants were treated for 24 hours with different stresses, (1) at 4 Grad C in the dark, (2), at 37 Grad C in the dark, (3) lying in the lab after removing from soil, (4) in the greenhouse after wounding leaves with a foreceps, (5) in the lab watering with a 150 mM NaCl solution, (6) at 26 Grad C in the light/UV; equal quantities of stressed plant material were pooled; library was made at the Max-Planck-Institute for Plant Breeding Research, Cologne, Germany; cloning sites SalI-NotI, primer sites and orientation: T7-SalI-CCACGCGTCCG-5prime-cDNA-polyA-CC-NotI-SP6; Note: Sequencing granted in the context of the GABI Arabidopsis Verbund I: Genetic Diversity, 'Establishment of high-efficiency SNP-based mapping tools and development of methods for genome-wide mutation detection' PI: Bernd Weisshaar Sequence submission managed by RZPD/GABI-Primary database: http://gabi.rzpd.de. This clone is available from RZPD; contact RZPD (clone@rzpd.de) for further information.

text: adult plant, mixed stresses

text: E. coli TOP10

text: Arabidopsis thaliana

text: SalI

text: NotI

text: whole plant

characteristic: vector


text: Bielefeld University

text: live

text: Generic

text: Niederzens-1 (Nd-1)

characteristic: cultivar

text: RNA from total, adult, 6 weeks old Arabidopsis thaliana (accession Nd-1) plants, grown in the greenhouse under long day conditions in soil treated for 24 hours with different stresses, (1) at 4 Grad C in the dark, (2), at 37 Grad C in the dark, (3) lying in the lab after removing from soil, (4) in the greenhouse after wounding with a foreceps, (5) in the lab watering with a 150 mM NaCl solution, (6) at 26 Grad C in the light/UV; equal quantities of stressed plant material were pooled; library was made at the Max-Planck-Institute for Plant Breeding Research, Cologne, Germany; cloning sites SalI-NotI, primer sites and orientation: T7-SalI-CCACGCGTCCG-5prime-cDNA-polyA-CC-NotI-SP6; Note: Sequencing granted in the context of the GABI Arabidopsis Verbund I: Genetic Diversity, 'Establishment of high-efficiency SNP-based mapping tools and development of methods for genome-wide mutation detection' PI: Bernd Weisshaar Sequence submission managed by RZPD/GABI-Primary database: http://gabi.rzpd.de. This clone is available from RZPD; contact RZPD (clone@rzpd.de) for further information.

text: adult plant, mixed stresses

text: E. coli TOP10

text: Arabidopsis thaliana

text: SalI

text: NotI

text: whole plant

characteristic: tissue-type


text: Bielefeld University

text: live

text: Generic

characteristic: NCBI submission model

text: Landsberg erecta (Ler)

text: cDNA library from Arabidopsis thaliana, accession Landsberg erecta; six weeks old total plants grown under long-day conditions in soil, whole adult plants were treated for 24 hours with different stresses, (1) at 4M-0 C in the dark, (2), at 37 Grad C in the dark, (3) lying in the lab after removing from soil, (4) in the greenhouse after wounding leaves with a foreceps, (5) in the lab watering with a 150 mM NaCl solution, (6) at 26 Grad C in the light/UV; equal quantities of stressed plant material were pooled; library was made at the Max-Planck-Institute for Plant Breeding Research, Cologne, Germany; cloning sites SalI-NotI, primer sites and orientation: T7-SalI-CCACGCGTCCG-5prime-cDNA-polyA-CC-NotI-SP6; Note: Sequencing granted in the context of the GABI Arabidopsis Verbund I: Genetic Diversity, 'Establishment of high-efficiency SNP-based mapping tools and development of methods for genome-wide mutation detection' PI: Bernd Weisshaar Sequence submission managed by RZPD/GABI-Primary database: http://gabi.rzpd.de. This clone is available from RZPD; contact RZPD (clone@rzpd.de) for further information.

text: adult plant, mixed stresses

text: E. coli XL1-Blue MRF

characteristic: lab-host

text: Arabidopsis thaliana

text: SalI

text: NotI

text: whole plant


text: Bielefeld University

text: live

text: Generic

characteristic: NCBI submission package

text: cDNA library from Arabidopsis thaliana, accession C24; seedling; Lambda ZAPII phage library was made at the Max-Planck-Institute of Molecular Plant Physiology, Golm, Germany and mass-excised at the Max-Planck-Institute for Plant Breeding Research, Cologne, Germany; cloning sites EcoRI-XhoI; Note: Sequencing granted in the context of the GABI Arabidopsis Verbund I: Genetic Diversity, 'Establishment of high-efficiency SNP-based mapping tools and development of methods for genome-wide mutation detection' PI: Bernd Weisshaar Sequence submission managed by RZPD/GABI-Primary database: http://gabi.rzpd.de This clone is available from RZPD; contact RZPD (clone@rzpd.de) for further information.

characteristic: description

text: few days old seedlings

text: E. coli XL1-Blue MRF

text: Arabidopsis thaliana

text: EcoRI

text: XhoI

text: seedling

text: pBluescript SK (-)


text: Bielefeld University

text: live

text: Generic

text: Achkarren-2 (Ak-2)

text: cDNA library from Arabidopsis thaliana, accession Achkarren-2; inflorescences from flower buds to young siliques; library was made at the Max-Planck-Institute for Plant Breeding Research, Cologne, Germany; cloning sites SalI-NotI, primer sites and orientation: T7-SalI-CCACGCGTCCG-5prime-cDNA-polyA-CC-NotI-SP6; Note: Sequencing granted in the context of the GABI Arabidopsis VerbundI: Genetic Diversity, 'Establishment of high-efficiency SNP-based mapping tools and development of methods for genome-wide mutation detection' PI: Bernd Weisshaar; Sequence submission managed by RZPD/GABI-Primary database: http://gabi.rzpd.de This clone is available from RZPD; contact RZPD (clone@rzpd.de) for further information.

text: E. coli TOP10

text: Arabidopsis thaliana

text: SalI

text: inflorescence


text: Kyoto University

text: live

text: Generic

text: hepatocellular carcinoma

characteristic: cell type

text: country: Japan:Kyoto

text: Homo sapiens

text: liver

text: Homo sapiens hepatocellular carcinoma Kyoto


text: University of Silesia

text: live

text: Generic

text: Karat

text: Library was made from cDNA RDA subtraction products carried out on root mRNA from wild type - parent variety Karat (TESTER) and hairless mutant 834Q (DRIVER).

text: 6-days seedling

text: Hordeum vulgare subsp. vulgare

characteristic: organism

text: root

text: Hordeum vulgare root EST library


text: Graduate School of Biochemical Sciences, Nagasaki University

text: live

text: Generic

text: adult

text: Mus musculus

text: male

characteristic: sex

text: BALB/slc

characteristic: strain

text: whole brain

text: Mus musculus whole brain BALB/slc adult


characteristic: External Id

text: Washington University School of Medicine

text: live

text: Generic

text: The library was constructed by Claire Murphy and Dr. James McCarter at Washington University, St. Louis. This is a Oligo(dT)-SL1 PCR based library. cDNA PCR products of size >400 nucleotides containing SL1 on the 5' end and oligo(dT) on the 3' end were non-directionally cloned into pCRII-TOPO(Invitrogen) following the Topo TA cloning protocol. Parasitic adult females were collected from immunologically naive animals and provided by Dr. Mark Viney (Mark.Viney@bristol.ac.uk) of University of Bristol, Bristol, UK.

text: Parasitic adult

text: Strongyloides ratti

text: EcoRI

text: EcoRI

text: female

text: Strongyloides ratti PA female naive SL1 TOPO v2

characteristic: title

text: plasmid (kanamycin and ampicillin resistant)

text: pCRII-TOPO (Invitrogen)


characteristic: INSDC center name

text: live

text: Generic

text: The library was constructed by Claire Murphy and Dr. James McCarter at Washington University, St. Louis. This is a Oligo(dT)-SL1 PCR based library. cDNA PCR products of size >400 nucleotides containing SL1 on the 5' end and oligo(dT) on the 3' end were non-directionally cloned into pCRII-TOPO(Invitrogen) following the Topo TA cloning protocol. Parasitic adult females were collected from partially immune animals and provided by Dr. Mark Viney (Mark.Viney@bristol.ac.uk) of the University of Bristol, Bristol, UK.

text: Parasitic adult

characteristic: dev-stage

text: Strongyloides ratti

text: EcoRI

text: EcoRI

text: female

text: Strongyloides ratti PA female partially immune SL1 TOPO v2

text: pCRII-TOPO (Invitrogen)


text: Medical College Of QingDao University

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: epithelium

text: RNA preparation. Total RNA was extracted from the clamworm digestive tract epitheliums by Trizol reagent(Invitrogen). Total RNA was digested with RNase-free DNase(Promege). Revers transcription-PCR(RT-PCR) cloning. Total RNA was revers-transcribed with an oligo(dT)15 and the murine leukemia virus reverse transcriptase(SuperscriptTM RNase H- Reverse Transcriptase, Invitrogen). The resulting cDNA was copied by the PCR with two primers designed on the basis of the conserved amino acid of serine protease and oligo(dT)21 as follows: sense from GDSGGP, 5' GGTGACTCYGGYGGCCCT 3', and antisense 5' TTTTTTTTTTTTTTTTTTTTT 3'. Rapid amplification of cDNA ends PCR(RACE PCR) cloning. RACE PCR was done using the GeneRacerTM Kit(Invitrogen). The products were subcloned into the T-A Easy Vector(Promega) and sequenced.

text: Perinereis aibuhitensis

text: ECORI

text: ECORI

text: digestive tract

text: clamworm digestive tract epithelium library

text: Plasmid

text: T-A Easy Vector


text: National Chemical Laboratory

text: live

characteristic: INSDC status

text: Generic

text: leaf tissue

text: cDNA from TcLr15 seedlings inoculated with Puccinia recondita Rob ex Desm. F.sp. tritici Ericks & Henn pathotype 21-R-55, subtracted with un-inoculated control cDNA. Leaf tissue harvested 48 hours after inoculation. Subtracted cDNAs cloned in pGMT Easy and maintained in E. coli DH5 alpha.

text: 48 hours post-inoculation

text: Triticum aestivum

text: Thatcher Lr15

text: leaf

text: Subtracted wheat leaf-rust inoculated TcLr15 cDNA library


text: INRA

text: live

text: Generic

text: A composite cDNA library was made with mRNA isolated from normal, compression, opposite, early and late wood of Maritime pine uni-directionally cloned into Uni-ZAP XR using the ZAP-cDNA Synthesis kit (Stratagene). pBluescript SK(-) plasmids were obtained by in vivo mass excision. The nucleotide sequence of the 5'end was obtained by automated sequencing with the T3 primer by GENOME EXPRESS, Meylan, France

text: adult

text: Pinus pinaster

text: Eco RI

text: Xho I

text: ecotype: Corsican

text: differenciating xylem

text: Pinus pinaster differenciating xylem adult

text: Uni-Zap XR lambda (Stratagene)

characteristic: vector


text: University of Maryland, Baltimore School of Medicine

text: live

text: Generic

text: cDNA subjected to PCR amplification with random primers and cloned into Topo TA vector.

text: Dermacentor variabilis

text: laboratory strain

text: Ovary

characteristic: tissue-type

text: Differentially expressed Dermacentor variabilis cDNA fragments


text: live

text: Generic

characteristic: NCBI submission model

text: This library was generated by cloning cDNAs directionally into Uni-ZAP(Stratagene) (T3 primer/EcoRI are at the 5'-end and T7/XhoI are at the 3'-end). The library was excised [now in pBluescript SK(+)] and normalized (Bonaldo et al 1996 Genome Research 6:791-806). Library constructed by Thomas Baum (tbaum@iastate.edu), Iowa State University, Plant Pathology Department and Jeff McDermott (jpmcderm@iastate.edu).

text: 3rd stage juvenile

text: Heterodera glycines

text: XhoI

text: EcoRI

text: mixed

text: whole organism

text: Heterodera glycines J3

text: plasmid (Ampicillin)

text: pBluescript SK+ (Stratagene)


text: ESALQ - University of Sao Paulo

text: live

text: Generic

text: SEL 1308

characteristic: cultivar

text: mRNA from bean tissue was extracted with the FastTrack 2.0 kit (Invitrogen) and mRNA sample quality was checked in 1.2% formaldehyde-agarose gel. cDNA was synthesized and cloned into the pCMV SPORT 6 vector with the SuperScript Plasmid System with Gateway Technology kit (Gibco BRL) as indicated by the manufacturer. Recombinant colonies were cultured in 96-well plates and maintained in liquid LB medium with ampicillin (100ug/ml) and 8% glycerol at -80oC. Plasmid miniprep was conducted in 96-well plates using standard procedure and sequencing of the cDNA was performed in 96-well PCR plates using the Big Dye Terminator Cycle Sequencing Ready Reaction Kit (Applied Biosystems). Clones were sequenced from the 5' end using M13 Reverse primer and from the 3' end using the T7 primer. PCR file consisted of 25 cycles of 10 sec. at 96oC, 5 sec. at 50oC, and 4 min. at 60oC. After PCR product precipitation, large-scale automated sequencing was carried out in a capillary system (3700 ABI Sequencer, Applied Biosystems). Raw data from sequencing was validated in a series of automated routine analyses including Phred/CAP3/Consed programs and Perl scripts created by our team. Reads that did not have at least 300 bases with Phred quality of 20 were not included in the analysis.

text: 19 days after seed germination

text: E. coli DH10B

characteristic: lab-host

text: Phaseolus vulgaris

text: Not I

text: Sal I

text: leaf

text: Common bean leaf EST Library - PVEPLE1

text: plasmid

text: pCMV SPORT 6


text: University of Kiel

text: live

text: Generic

characteristic: NCBI submission package

text: roots

characteristic: organ

text: Beta vulgaris

text: Beta vulgaris TDFs expressed upon Heterodera schachtii infection

text: Plasmid

text: pGEM-T


text: live

text: Generic

text: Nosema bombycis

characteristic: organism

text: Nosema bombycis cDNA library


text: National University of Singapore

text: live

text: Generic

text: Blomia tropicalis

text: Blomia tropicalis cDNA library


text: National University of Singapore

text: live

text: Generic

text: Library was prepared by Tsai, L.C. et al., Veteran's General Hospital, Taipei, Taiwan.

characteristic: description

text: Dermatophagoides farinae

text: Dermatophagoides farinae cDNA library


text: INRA-Agrocampus-Rennes Genetique Animale

text: live

text: Generic

text: Clone distribution : AGENAE Resource centre. Francois PIUMI, Francois.Piumi.inra.fr, INRA, CEA Radiobiologie et Etude du genome (LREG), Domaine de Vilvert, 78352, Jouy-en-Josas cedex, FRANCE

text: from embryos to adults

text: Gallus gallus

text: adrenal gland, caecum, duodenum, jejunum, liver, multi-tissues

text: AGENAE Gallus gallus multi-tissues library (gcae)


text: live

text: Generic

text: adult

text: Gallus gallus

text: adipose tissue, granulosa, multi-tissues, oviduct, small folicule, utero-vaginal gland

text: AGENAE Gallus gallus multi-tissues library (gcab)


text: live

text: Generic

text: from embryos to adults

text: Gallus gallus

text: brain, multi-tissues, ovary, embryos, hypothalamus, skin, pituitary gland

text: AGENAE Gallus gallus multi-tissues library (gcac)


text: live

text: Generic

text: from embryos to adults

text: Gallus gallus

text: ileon, kidney, multi-tissues, pancreas, testis, hematopoietic progenitor cells

text: AGENAE Gallus gallus multi-tissues library (gcad)


text: live

text: Generic

text: adult

text: Gallus gallus

text: bone marrow, fabricius gland, multi-tissues, muscle, spleen, thymus

text: AGENAE Gallus gallus multi-tissues library (gcaf)


text: live

text: Generic

text: tissue: adipose tissue, brain, intestine, kidney, liver, multi-tissues, muscle, ovary, testis, adrenal gland, bone marrow, caecum, duodenum, embryos, fabricius gland, genital track, granulosa, hypothalamus, ileon, jejunum, lymphoid tissue, oviduct, pancreas, skin, spleen, thymus, utero-vaginal gland, pituitary gland, small follicle, hematopietic progenitor cells. Clone distribution: AGENAE Resource centre. Francois PIUMI, Francois.Piumi@jouy.inra.fr, INRA, CEA Radiobiologie et Etude du genome (LREG), Domaine de Vilvert, 78352, Jouy-en-Josas cedex, FRANCE, +33 (0) 1.34.65.28.02, +33 (0) 1.34.65.22.73

text: from embryos to adults

text: Gallus gallus

text: multi-tissues

text: AGENAE Gallus gallus multi-tissues normalized library (gcag)


text: live

text: Generic

text: tissue: embryos, hypothalamus, ovary, skin, bone marrow, fabricius gland, multi-tissues, muscle, spleen, thymus, small follicle, hematopietic progenitor cells, adipose tissue, granulosa, utero-vaginal gland, oviduct, pituitary gland, pancreas, testis, ileon, kidney, liver, jejunum, caecum, duodenum. Clone distribution : AGENAE Resource centre. Francois PIUMI, Francois.Piumi@jouy.inra.fr, INRA, CEA Radiobiologie et Etude du genome (LREG), Domaine de Vilvert, 78352, Jouy-en-Josas cedex, FRANCE, +33 (0) 1.34.65.28.02, +33 (0) 1.34.65.22.73

text: from embryos to adults

text: Gallus gallus

text: multi-tissues

text: AGENAE Gallus gallus multi-tissues normalized and once-subtracted cDNA library (gcal)


text: live

text: Generic

text: adult

text: Gallus gallus

text: adipose tissue, multi-tissues, muscle, bone marrow, fabricius gland, granulosa, oviduct, small folicule, spleen, thymus, utero-vaginal gland

text: AGENAE Gallus gallus library (gcah)


text: Department of Microbiology and Immunology, University of Maryland, Baltimore, School of Medicine

text: live

text: Generic

text: Homologue cloned partial cDNA sequences obtained by degenerate primer design, RACE amplification, TOPO TA cloning, and the dye terminator method of automated sequencing.

text: Adult

text: Dermacentor variabilis

text: female

characteristic: sex

text: laboratory strain

characteristic: strain

text: Five day partially fed whole tick mRNA

text: Homologue cloned Dermacentor variabilis mRNAs


characteristic: External Id

text: LG Molekulargenetik

text: live

text: Generic

text: genotype A17; Total RNA was isolated from roots harvested 6 days after inoculation with Aphanomyces euteiches zoospores. cDNA was prepared from total RNA using the SMART PCR cDNA system (Clontech). This cDNA was used as tester in Suppression Substractive Hybridization (SSH). The SSH-cDNA fragments were generated using the SSH-adaptor sequences ctaatacgactcactatagggctcgagcggccgcccgggcaggt and ctaatacgactcactatagggcagcgtggtcgcggccgaggt (Clontech) and ligated after Suppression Subtractive Hybridization into the pGEM-Teasy vector (Promega). Plasmids containing cDNA inserts were propagated in E. coli TOP 10F' cells (InVitrogen).

text: 6 days afer inoculation with Aphanomyces euteiches zoospores

text: Medicago truncatula

text: EcoRI

text: root

text: MTAPHEU

characteristic: title

text: pGEM-T


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

characteristic: INSDC center name

text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Normal adult pancreatic islets, estimated to be 90% pure, were source of mRNA. Uni-ZAP XR Vector. Reference: Human Molecular Genetics 2, 1793-1798 (1993) Library constructed by J. Takeda, G. Bell. 5' adaptor sequence: 5' GAATTCGGCACGAG 3' 3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'

text: adult

characteristic: dev-stage

text: SOLR (kanamycin resistant)

text: Homo sapiens

text: pancreas, islet

text: Takeda/Bell pancreatic islet


text: Department of Botany & Plant Sciences, University of California

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Washington Navel

text: Plants were grown in the field at University of California, Riverside Agricultural Operations since 1983. The scion was Washington Navel orange and the rootstock Carizzo Citrange. Tissue from mature fruit was harvested at mid-day in January 2002 during a cold spell, when pre-dawn temperatures were approximately -2 to 2 degree C. Approximately 2 cm median sections of the rind were excised in the field from several fruits, then wrapped in aluminum foil and frozen quickly in dry ice. Total RNA was extracted using a phenol extraction procedure described in J. Japanaese Soc. Hort. Sci. (1996) 64:809-814. Poly(A) RNA was purified, a cDNA library was made, and 1 million primary lambda cDNA clones were in vivo excised to give a population of pBluescript SK(-) phagemids. All steps to this point were performed in the TJ Close lab at the University of California, Riverside (Fenton). Phagemids were plated, plasmid DNA purified, cDNA clones archived, and DNA sequences determined bi-directionally using an ABI3530 at the Arizona Genomics Institute, University of Arizona (Collura, Feuerbacher, Kim, Kudrna, Wing, Yu). Chromatogram files were transmitted to UC Riverside (by Yu), then processed at UC Riverside (by Wanamaker) using the HarvEST pipeline (http://harvest.ucr.edu) to remove vector and cloning oligo sequences and various contaminants, and to trim to a high quality region. Sequences that retained a phred 17 region of at least 100 bases were deposited to GenBank.

text: Mature fruit

text: E. coli TJC121

text: Citrus sinensis

text: EcoRI

text: XhoI

text: Rind containing flavedo and albedo

text: Washington Navel orange cold acclimated flavedo & albedo cDNA library

text: Phagemid pBluescript SK(-)

text: Lambda Uni-ZAP XR, excised phagemid


text: University of Wisconsin, Madison

text: live

characteristic: INSDC status

text: Generic

text: Monosiga brevicollis

text: NotI

text: SalI

text: ATCC 50154

text: plasmid

text: pSportI

characteristic: vector


text: University of Wisconsin, Madison

text: live

text: Generic

characteristic: NCBI submission model

text: Salpingoeca rosetta

text: NotI

text: SalI

text: ATCC 50818

text: plasmid


text: Max-Planck-Institute for evolutionary Anthropology

text: live

text: Generic

text: cDNA was prepared by primer extension after reverse transcription.

text: adult

text: Epicurian Coli (TM) XL-10-Gold

text: Pan troglodytes

text: SfiI-A

text: SfiI-B

text: female

text: brain, presumably cortex

characteristic: tissue-type

text: Chimpanzee brain library Angela

text: Plasmid

text: pUChi


text: live

text: Generic

text: The library was prepared using the SMART cDNA library construction Kit (Clontech), doing only primer extension, but not PCR amplification of the cDNA. The only deviation from the published protocoll was that we cloned the cDNA into a plasmid Vector.

text: adult

text: Epicurian Coli (TM) XL-10-Gold

characteristic: lab-host

text: Pan troglodytes

text: SfiI-A

text: SfiI-B

text: male

text: brain, presumably cortex

text: Chimpanzee brain library Koos

text: Plasmid

text: pUChi


text: live

text: Generic

characteristic: NCBI submission package

text: adult

text: Epicurian Coli (TM) XL-10-Gold

text: Pan troglodytes

text: SfiI-A

text: SfiI-B

text: male

text: testis

text: Chimpanzee testis library Koos

text: Plasmid

text: pUChi


text: UC Davis, Department of Pomology

text: live

text: Generic

text: Testa

characteristic: cell type

text: Tulare

characteristic: cultivar

text: Walnut nut samples were harvested from Tulare trees growing in the 'Stuke block' in the Wolfskill experimental orchard located in Winters, California (USA). Samples were harvested on August 1, 2001 between 8 and 10 am. Samples were then dissected the same day. Pellicle tissue was separated from embryos and frozen immediately in liquid nitrogen and stored at -80C. A gram of sample was removed and ground to a fine powder in liquid nitrogen. Total RNA was extracted using the hot borate procedure. Poly A+ RNA was obtained using the Poly(A) Purist kit (Ambion). The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). Primary library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes.

text: Mid-season fruit - collected August 1, 2001

text: Seed coat

characteristic: organ

text: Juglans regia

text: EcoRI

text: XbaI

text: Hermaphrodite

text: Tulare pellicle library

text: Plasmid


text: UC Davis, Department of Pomology

text: live

text: Generic

text: Flavedo

text: Washington navel orange

text: Mature citrus fruits were harvested from trees growing in the 'Citrus variety collection' in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected in January 2002, between 1 and 3 PM and stored at 4C. Flavedo tissue was separated from the rest of the peel and used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The amplified library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes.

characteristic: description

text: Mature fruit sample - collected January 2002

text: Peel/rind

text: Citrus sinensis

characteristic: organism

text: EcoRI

text: XbaI

text: Hermaphrodite

characteristic: sex

text: Flavedo Mature

text: Plasmid


characteristic: External Id

text: University of Chicago Medical Center

text: live

text: Generic

text: Drosophila melanogaster

text: Drosophila melanogaster cDNA Library

characteristic: title

text: Plasmid


text: Lethbridge Research Centre, AAFC

characteristic: INSDC center name

text: live

text: Generic

text: cDNA library prepared from protozoa recoverd from rumen fluid

text: Isotricha sp. BBF-2003

text: Iso cDNA

text: plasmid

text: pGEM-T


text: Jawaharlal Nehru University

characteristic: INSDC first public

text: live

text: Generic

text: Xanthii

text: E.coli

text: Nicotiana tabacum

text: Regenerating tissue

text: Nicotiana tabaccum PCR-based subtractive library

text: Plasmid

text: p-GEMT Easy


characteristic: INSDC last update

text: live

text: Generic

text: 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.23 kb (range 0.5-4.0 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA).

text: Rattus norvegicus

text: SfiI

text: SfiI

text: Pitutary

text: plasmid (Chloramphenicol resistant)

text: pDNR-LIB


text: live

characteristic: INSDC status

text: Generic

text: 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.4 kb (range 0.6-3.5 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA). Note: this is a NIH_MGC Library.

text: DH10B (T1-phage-resistant)

text: testis

text: Mus musculus

text: SfiI (ggccattatggcc)

text: SfiI (ggccgcctcggcc)

text: primary cultures of Sertoli cells

text: plasmid (chloramphenicol resistant, grown at 30C)

text: pDNR-LIB


text: live

text: Generic

text: Normalized, full-length enriched library from pool of mouse embronic limb, maxilla and mandible, embryonic day 17.5, 18.5 and newborn (mandible (5, 4 and 1 limb and jaw equivalents from respective days). Cloned directionally, oligo-dT primed (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3'. Size selected for the >1kb fragments, average insert size 1.2 kb. Normalization to Cot 7.5 . Tissue contributed by David Rowe; library constructed by ResGen, Invitrogen Corp. Note: this is a NIH_MGC Library.

text: DH10B (phage-resistant)

text: Mus musculus

text: EcoRV

text: NotI

text: embryonic limb, maxilla and mandible

text: phagemid (ampicillin resistant)

characteristic: vector


text: Korea University

text: live

text: Generic

characteristic: NCBI submission model

text: For construction of the cDNA library, the tissues beneath the leaf sheaths at the base of the plants were harvested at 3, 4, 5, and 6 days afte the plants were infested by larvae of Hessain fly biotype L

text: seedling grown for 3 weeks

characteristic: dev-stage

text: XL1-Blue MRF'

text: Triticum aestivum

text: Hessian fly infested stems

text: Hessian fly infested cDNA library

text: pBluescripts SK(+/-) phagemid


text: University of Florida, IFAS

text: live

text: Generic

text: Valencia

text: 24 hr after application of CMNP abscission agent

text: Citrus sinensis

text: leaf blade

characteristic: tissue-type

text: Mature leaf blade cDNA subtraction library


text: Washington University School of Medicine

text: live

text: Generic

characteristic: NCBI submission package

text: The library was constructed by Amy Rush, Claire Murphy and Dr. James McCarter at Washington University, St. Louis. The cDNA was made by using Dynabead oligo-dT priming (Dynal). PCR based library using a modified protocol from the SMART PCR cDNA Synthesis Kit from Clontech. Directionally cloned into the UDG sites of pAMP1. Two day irradiated nematodes were provided by Dr. Gary Weil of Washington University, St. Louis, MO (weillab@imgate.wustl.edu).

text: irradiated 2 day cultured L3

characteristic: lab-host

text: Brugia malayi

text: NotI

text: SalI

text: Brugia malayi 2D L3 irradiated pAMP1 v2

text: plasmid (ampicillin resistant)

text: pAMP1 (Gibco)