text: live

text: Generic

text: 15 days embryo

text: Mus musculus

text: liver

text: RIKEN full-length enriched mouse cDNA library, C57BL/6J liver 15 days embryo


status: PUBLIC

characteristic: External Id

text: The Institute of Physical and Chemical Research (RIKEN)

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: adult

tag: attribute

characteristic: dev-stage

text: Mus musculus

characteristic: organism

text: male

characteristic: sex

characteristic: strain

text: liver

characteristic: tissue-type

text: RIKEN full-length enriched mouse cDNA library, C57BL/6J liver male adult

characteristic: title


text: live

text: Generic

text: adult

text: Mus musculus

text: male

text: lung

text: RIKEN full-length enriched mouse cDNA library, C57BL/6J lung male adult


text: live

text: Generic

text: 11 days pregnant adult

text: Mus musculus

text: female

text: placenta

text: RIKEN full-length enriched mouse cDNA library, C57BL/6J placenta female 11 days pregnant adult


text: live

text: Generic

text: 12 days pregnant adult

text: Mus musculus

text: female

text: placenta

text: RIKEN full-length enriched mouse cDNA library, C57BL/6J placenta female 12 days pregnant adult


text: live

text: Generic

text: 13 days pregnant adult

text: Mus musculus

text: female

text: placenta

text: RIKEN full-length enriched mouse cDNA library, C57BL/6J placenta female 13 days pregnant adult


text: live

text: Generic

text: 14 days pregnant adult

text: Mus musculus

text: female

text: placenta

text: RIKEN full-length enriched mouse cDNA library, C57BL/6J placenta female 14 days pregnant adult


text: live

text: Generic

text: 15 days pregnant adult

text: Mus musculus

text: female

text: placenta

text: RIKEN full-length enriched mouse cDNA library, C57BL/6J placenta female 15 days pregnant adult


text: live

text: Generic

text: adult

text: Mus musculus

text: male

text: spleen

text: RIKEN full-length enriched mouse cDNA library, C57BL/6J spleen male adult


text: live

text: Generic

text: 17 days embryo

text: Mus musculus

text: stomach

text: RIKEN full-length enriched mouse cDNA library, C57BL/6J stomach 17 days embryo


text: live

text: Generic

text: 10 days neonate

text: Mus musculus

text: thymus

text: RIKEN full-length enriched mouse cDNA library, C57BL/6J thymus 10 days neonate


text: live

text: Generic

text: 14 days embryo

text: Mus musculus

text: whole body

text: RIKEN full-length enriched mouse cDNA library, C57BL/6J whole body 14 days embryo


text: live

text: Generic

text: 15 days embryo

text: Mus musculus

text: whole body

text: RIKEN full-length enriched mouse cDNA library, C57BL/6J whole body 15 days embryo


text: live

text: Generic

text: 16 days embryo

text: Mus musculus

text: whole body

text: RIKEN full-length enriched mouse cDNA library, C57BL/6J whole body 16 days embryo


text: live

text: Generic

text: 17 days embryo

text: Mus musculus

text: whole body

text: RIKEN full-length enriched mouse cDNA library, C57BL/6J whole body 17 days embryo


text: live

text: Generic

text: 17.5 days embryo

text: Mus musculus

text: whole body

text: RIKEN full-length enriched mouse cDNA library, C57BL/6J whole body 17.5 days embryo


text: live

text: Generic

text: 18 days embryo

text: Mus musculus

text: whole body

text: RIKEN full-length enriched mouse cDNA library, C57BL/6J whole body 18 days embryo


text: The Institute of Physical and Chemical Research (RIKEN)

text: live

text: Generic

text: CRL-1722 L5178Y-R

characteristic: cell-line

text: Mus musculus

text: RIKEN full-length enriched mouse cDNA library, DBA/2 CRL-1722 L5178Y-R


text: live

text: Generic

text: B16 F10Y cells

characteristic: cell type

text: Mus musculus

text: RIKEN full-length enriched mouse cDNA library, C57BL/6J B16 F10Y cells


text: live

text: Generic

text: macrophage

text: Mus musculus

text: bone marrow

text: RIKEN full-length enriched mouse cDNA library, C57BL/6J bone marrow macrophage


text: live

text: Generic

text: mast cells

text: Mus musculus

text: bone marrow

text: RIKEN full-length enriched mouse cDNA library, C57BL/6J bone marrow mast cells


text: live

text: Generic

text: erythroblast

text: Mus musculus

text: RIKEN full-length enriched mouse cDNA library, C57BL/6J erythroblast


text: live

text: Generic

text: adult

text: Mus musculus

text: inner ear

text: RIKEN full-length enriched mouse cDNA library, C57BL/6J inner ear adult


text: live

text: Generic

text: adult

text: Mus musculus

text: male

text: liver tumor

text: RIKEN full-length enriched mouse cDNA library, C57BL/6J liver male adult tumor


text: live

text: Generic

text: melanocyte

text: Mus musculus

text: RIKEN full-length enriched mouse cDNA library, C57BL/6J melanocyte


text: live

text: Generic

text: olfactory sensory neurons

text: Mus musculus

text: olfactory mucosa

text: RIKEN full-length enriched mouse cDNA library, C57BL/6J olfactory mucosa olfactory sensory neurons


text: live

text: Generic

text: osteoclast-like cell

text: Mus musculus

text: RIKEN full-length enriched mouse cDNA library, C57BL/6J osteoclast-like cell


text: live

text: Generic

text: islet cells

text: adult

text: Mus musculus

text: pancreas

text: RIKEN full-length enriched mouse cDNA library, C57BL/6J pancreas adult islet cells


text: live

text: Generic

text: parthenogenote 9.5 days embryo

text: Mus musculus

text: RIKEN full-length enriched mouse cDNA library, C57BL/6J parthenogenote 9.5 days embryo


text: live

text: Generic

text: adult

text: Mus musculus

text: retina

text: RIKEN full-length enriched mouse cDNA library, C57BL/6J retina adult


text: live

text: Generic

text: Th2 CD4+Tcell

text: Mus musculus

text: spleen

text: RIKEN full-length enriched mouse cDNA library, C57BL/6J spleen Th2 CD4+Tcell


text: live

text: Generic

text: stroma cell

text: Mus musculus

text: RIKEN full-length enriched mouse cDNA library, C57BL/6J stroma cell


text: live

text: Generic

text: 2 days neonate

text: Mus musculus

text: sympathetic ganglion

text: RIKEN full-length enriched mouse cDNA library, C57BL/6J sympathetic ganglion 2 days neonate


text: The Institute of Physical and Chemical Research (RIKEN)

text: live

text: Generic

text: thymic cells

text: 2 days neonate

text: Mus musculus

text: thymus

text: RIKEN full-length enriched mouse cDNA library, C57BL/6J thymus 2 days neonate thymic cells


text: live

text: Generic

text: 3 days neonate

text: Mus musculus

text: thymus

text: RIKEN full-length enriched mouse cDNA library, C57BL/6J thymus 3 days neonate


text: live

text: Generic

text: Mus musculus

text: thymus

text: RIKEN full-length enriched mouse cDNA library, C57BL/6J thymus CD4+CD8+Tcells


text: live

text: Generic

text: Mus musculus

text: visual cortex

text: RIKEN full-length enriched mouse cDNA library, C57BL/6J visual cortex


text: live

text: Generic

text: 12.5 days embryo

text: Mus musculus

text: Rathke's pouches

text: RIKEN full-length enriched mouse cDNA library, CD-1 Rathke's pouches 12.5 days embryo


text: live

text: Generic

text: NOD-derived CD11c +ve dendritic cells

text: Mus musculus

text: NOD

text: RIKEN full-length enriched mouse cDNA library, NOD NOD-derived CD11c +ve dendritic cells


text: live

text: Generic

text: Mus musculus

text: NOD

text: activated spleen

text: RIKEN full-length enriched mouse cDNA library, NOD activated spleen


text: live

text: Generic

text: thymic cells

text: 2 days neonate

text: Mus musculus

text: NOD

text: thymus

text: RIKEN full-length enriched mouse cDNA library, NOD thymus 2 days neonate thymic cells


text: live

text: Generic

text: 14.5 days embryo

text: Mus musculus

text: Rathke's pouches

text: RIKEN full-length enriched mouse cDNA library, Rathke's pouches 14.5 days embryo


text: live

text: Generic

text: synovial fibroblasts

text: Mus musculus

text: RIKEN full-length enriched mouse cDNA library, synovial fibroblasts


text: live

text: Generic

text: Mus musculus

text: whole joints

text: RIKEN full-length enriched mouse cDNA library, whole joints


text: live

text: Generic

text: adult

text: Mus musculus

text: female

text: uterus

text: RIKEN full-length enriched mouse cDNA library, C57BL/6J uterus female adult


text: live

text: Generic

text: Th1 CD4+Tcells

text: Mus musculus

text: spleen

text: RIKEN full-length enriched mouse cDNA library, C57BL/6J spleen Th1 CD4+Tcells


text: live

text: Generic

text: B6-derived CD11 +ve dendritic cells

text: Mus musculus

text: RIKEN full-length enriched mouse cDNA library, C57BL/6J B6-derived CD11 +ve dendritic cells


text: live

text: Generic

text: (dev_stage=14 days embryo,tissue_type=liver,sex=mix),(dev_stage=11 days pregnant, adult,tissue_type=placenta,sex=female), (dev_stage=12 days pregnant, adult,tissue_type=placenta,sex=female),(dev_stage=13 days pregnant, adult,tissue_type=placenta,sex=female), (dev_stage=14 days pregnant adult,tissue_type=placenta,sex=female),(dev_stage=15 days pregnant adult,tissue_type=placenta,sex=female), (dev_stage=14 days pregnant, adult,tissue_type=amnion,sex=female)

characteristic: description

text: Mus musculus

text: RIKEN full-length enriched mouse cDNA library, pooled tissue 6


text: The Institute of Physical and Chemical Research (RIKEN)

text: live

text: Generic

text: (dev_stage=15 days embryo,tissue_type=brain,sex=mix),(dev_stage=14 days embryo,tissue_type=liver,sex=mix),(dev_stage=14 days pregnant adult,tissue_type=placenta,sex=female), (dev_stage=15 days pregnant adult,tissue_type=placenta,sex=female),(dev_stage=adult, tissue_type=kidney,sex=male),(dev_stage=14 days embryo,tissue_type=whole body,sex=mix),(dev_stage=16 days embryo,tissue_type=whole body,sex=mix),(dev_stage=17 days embryo,tissue_type=whole body,sex=mix),(dev_stage=15 days pregnant adult,tissue_type=amnion,sex=female), (dev_stage=11 days embryo,tissue_type=whole body,sex=mix),(dev_stage=12 days embryo,tissue_type=whole body,sex=mix),(dev_stage=15 days embryo,tissue_type=whole body,sex=mix),(dev_stage=18 days embryo,tissue_type=whole body,sex=mix),(dev_stage=14 days embryo,tissue_type=brain,sex=mix),(dev_stage=15 days embryo,tissue_type=liver,sex=mix)

text: Mus musculus

text: RIKEN full-length enriched mouse cDNA library, C57BL/6J pooled tissue 7


text: live

text: Generic

text: (dev_stage=16 days embryo,tissue_type=heart,sex=mix),(dev_stage=16 days embryo,tissue_type=kidney,sex=mix),(dev_stage=17 days embryo,tissue_type=heart,sex=mix),(dev_stage=17 days embryo,tissue_type=stomach,sex=mix),(dev_stage=17 days embryo,tissue_type=kidney,sex=mix),(dev_stage=17 days pregnant, adult,tissue_type=amnion,sex=female), (dev_stage=13 days embryo,tissue_type=liver,sex=mix)

text: Mus musculus

text: RIKEN full-length enriched mouse cDNA library, pooled tissue 8


text: live

text: Generic

text: (sex=mix,cell_line=TIB-55 BB88),(sex=mix,cell_line=CRL-1722 L5178Y-R)

text: Mus musculus

text: RIKEN full-length enriched mouse cDNA library, pooled tissue 9


text: live

text: Generic

text: (dev_stage=adult,tissue_type=spleen,sex=male), (dev_stage=adult,tissue_type=kidney,sex=male), (dev_stage=adult,tissue_type=testis,sex=male), (dev_stage=adult,tissue_type=thymus,sex=male), (dev_stage=adult,tissue_type=heart,sex=male), (dev_stage=adult,tissue_type=colon,sex=male), (dev_stage=adult,tissue_type=stomach,sex=male), (dev_stage=adult,tissue_type=liver,sex=male), (dev_stage=13 days embryo,tissue_type=whole body,sex=mix),(dev_stage=14 days embryo,tissue_type=whole body,sex=mix),(dev_stage=16 days embryo,tissue_type=whole body,sex=mix),(dev_stage=17 days embryo,tissue_type=whole body,sex=mix),(dev_stage=15 days pregnant adult,tissue_type=amnion,sex=female),(dev_stage=10 days neonate,tissue_type=brain,sex=mix),(dev_stage=10 days neonate,tissue_type=thymus,sex=mix),(dev_stage=10 days neonate,tissue_type=heart,sex=mix)

text: Mus musculus

text: RIKEN full-length enriched mouse cDNA library, pooled tissue 10


text: live

text: Generic

text: (dev_stage=14 days pregnant adult,tissue_type=placenta,sex=female),(dev_stage=adult, tissue_type=brain,sex=male)

text: Mus musculus

text: RIKEN full-length enriched mouse cDNA library, C57BL/6J pooled tissue 11


text: live

text: Generic

text: (tissue_type=thymus,cell_type=CD4+CD8+Tcells), (tissue_type=spleen,cell_type=Th1 CD4+Tcells),(tissue_type=spleen,cell_type=Th2 CD4+Tcell)

text: Mus musculus

text: RIKEN full-length enriched mouse cDNA library, pooled tissue 12


text: USDA, ARS, NADC

text: live

text: Generic

text: The Lymph node SSH library is derived from the suppression subtractive hybridization between Salmonella enterica serotype Choleraesuis-infected and Salmonella enterica serotype Typhimurium-infected porcine lymph node.

text: DH5a (Life Technologies)

characteristic: lab-host

text: Sus scrofa

text: crossbreed

text: Lymph Node

text: SSH-ST-SC

text: pBAD (Invitrogen)

characteristic: vector


characteristic: External Id

text: Washington University School of Medicine

characteristic: INSDC center name

text: live

text: Generic

text: nyu.edu) of New York University.

text: Mixed

text: GC10 cells

text: Caenorhabditis japonica

text: SfiI B

text: SfiI A

text: plasmid (chloramphenical resistant)

text: pDNR-LIB


characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: cott@jhsph.edu) of the School of Public Hygene and Public Health at John Hopkins University in Baltimore, MD.

text: Adult

text: GC10 cells

text: Ascaris suum

text: SfiI B

text: SfiI A

text: Female

text: pharynx

text: Phagemid(chloramphenical resistant)

text: pDNR-LIB


text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: scott@jhsph.edu) of the School of Public Hygene and Public Health at John Hopkins University in Baltimore, MD.

text: Adult

text: GC10 cells

text: Ascaris suum

text: SfiI B

text: SfiI A

text: Male

text: pharynx

text: plasmid (chloramphenical resistant)

text: pDNR-LIB


text: North Carolina State University

text: live

text: Generic

characteristic: NCBI submission package

text: Beauregard

characteristic: cultivar

text: Ipomoea batatas

text: EcoRI

text: XhoI

text: Storage Root

text: storage root cDNA library

text: phagemid

text: pBK-CMV


text: Kunming Insitute of Botany, Chinese Academy of Sciences

text: live

text: Generic

text: Asarum caudigerum

characteristic: organism

text: Specific subtracted floral cDNA library of Asarum caudigerum


text: Fred Hutchinson Cancer Research Center

text: live

text: Generic

text: Library was constructed using Clontechs SMART cDNAlibrary construction kit. 0.5ug of total RNA from pooled coagulating glands, ventral prostate, and dorsolateral prostate from 10 adult male BalbC mice was used in 1st strand cDNA synthesis. 2nd strand cDNA was generated by 22 cycles of PCR, digested with SfiI and ligated into the lambdaTriplEx2 phagemid. Phagemids were converted to pTriplEx2 plasmids in the BM25.8 e-coli strain. Created by Colin Pritchard.

text: prostate

characteristic: organ

text: Mus musculus

text: SfiI

text: SfiI

text: Male

characteristic: sex

text: BalbC

characteristic: strain

text: Pooled coagulating glands (anterior prostate), ventral prostate, dorsolateral prostate

text: FH BalbClig05

text: plasmid (ampicillin resistant)


text: Fred Hutchinson Cancer Research Center

text: live

text: Generic

text: Library was constructed using Clontechs SMART cDNA library construction kit. 0.5ug of total RNA from pooled coagulating glands, ventral prostate, and dorsolateral prostate was used in 1st strand cDNA synthesis. 2nd strand cDNA was generated by 22 cycles of PCR, digested with SfiI and ligated into the lambdaTriplEx2 phagemid.Phagemids were converted to pTriplEx2 plasmids in the BM25.8 e-coli strain. Created from pooled coagulating glands (anterior prostate), ventral prostate and dorsolateral prostate from ten adult male mice by Colin Pritchard.

text: prostate

text: Mus musculus

text: SfiI

text: SfiI

text: Male

text: BalbC

text: Pooled coagulating glands (anterior prostate), ventral prostate and dorsolateral prostate from ten adult male mice

characteristic: tissue-type

text: FH BalbClig15

characteristic: title

text: plasmid (ampicillin resistant)


text: Fred Hutchinson Cancer Research Center

text: live

text: Generic

text: Library was constructed using Clontechs SMART cDNA library construction kit. 1ug of total RNA from pooled coagulating glands was used in 1st strand cDNA synthesis. 2nd strand cDNA was generated by 18 cycles of PCR, digested with SfiI and ligated into the lambdaTriplEx2 phagemid. Phagemids were converted to pTriplEx2 plasmids in the BM25.8 e-coli strain. Created from pooled coagulating glands from 3 month old male C57BL6 mice by Colin Pritchard.

characteristic: description

text: prostate

text: Mus musculus

text: SfiI

text: SfiI

text: Male

text: Pooled coagulating glands (anterior prostate)

text: FH CG02

text: plasmid (ampicillin resistant)


text: Fred Hutchinson Cancer Research Center

text: live

text: Generic

text: Library was constructed using Clontechs SMART cDNA library construction kit. RNA was DNase treated prior to 1st strand cDNA synthesis. 0.33ug of total RNA from pooled 10-day dorsolateral prostate, 0.33ug of total RNA from pooled 10-day ventral prostate, and 0.33ug of total RNA from pooled 10-day coagulating gland (anterior prostate) was combined and used in 1st strand cDNA synthesis. 2nd strand cDNA was generated by 21 cycles of PCR, digested with SfiI and ligated into the lambdaTriplEx2 phagemid. Phagemids were converted to pTriplEx2 plasmids in the BM25.8 e-coli strain. Created by Colin Pritchard.

characteristic: lab-host

text: prostate

text: Mus musculus

text: SfiI

text: SfiI

text: Male

text: pooled coagulating gland (anterior prostate), dorsolateral prostate, and ventral prostate from give 10-day old male mice

text: FH DAY10

text: plasmid (ampicillin resistant)


text: Fred Hutchinson Cancer Research Center

text: live

text: Generic

text: Library was constructed using Clontechs SMART cDNA library construction kit. RNA was DNase treated prior to 1st strand cDNA synthesis. 0.33ug of total RNA from pooled 20- day dorsolateral prostate, 0.33ug of total RNA from pooled 20-day ventral prostate, and 0.33ug of total RNA from pooled 20-day coagulating gland (anterior prostate) was combined and used in 1st strand cDNA synthesis. 2nd strand cDNA was generated by 21 cycles of PCR, digested with SfiI and ligated into the lambdaTriplEx2 phagemid. Phagemids were converted to pTriplEx2 plasmids in the BM25.8 e-coli strain. Created from pooled prostate lobes from five 20-day old mice by Colin Pritchard.

text: prostate

text: Mus musculus

text: SfiI

text: SfiI

text: Male

text: Equal amounts of pooled ventral prostate, dorsolateral prostate, and coagulating gland (anterior prostate) from five 20-day old male mice

text: FH DAY20

text: plasmid (ampicillin resistant)

characteristic: vector


characteristic: External Id

text: Fred Hutchinson Cancer Research Center

text: live

text: Generic

text: Library was constructed using Clontechs SMART cDNA library construction kit. RNA was DNase treated prior to 1st strand cDNA synthesis. 0.5ug of total RNA from pooled 35-day dorsolateral prostate, 0.5ug of total RNA from pooled 35-day ventral prostate, and 0.5ug of total RNA from pooled 35-day coagulating gland (anterior prostate) was combined and used in 1st strand cDNA synthesis. 2nd strand cDNA was generated by 21 cycles of PCR, digested with SfiI and ligated into the lambdaTriplEx2 phagemid. Phagemids were converted to pTriplEx2 plasmids in the BM25.8 e-coli strain. Created from pooled prostate from five 35 day old male C57BL6 mice by Colin Pritchard.

text: prostate

text: Mus musculus

text: SfiI

text: SfiI

text: Male

text: Equal amounts of pooled ventral prostate, dorsolateral prostate, and coagulating gland (anterior prostate) from five 35 day old male mice

text: FH DAY35

text: plasmid (ampicillin resistant)


text: Fred Hutchinson Cancer Research Center

characteristic: INSDC center name

text: live

text: Generic

text: Library was constructed using Clontechs SMART cDNA library construction kit. 0.5ug of total RNA from pooled 35-day dorsolateral prostate, 0.5ug of total RNA from pooled 35-day ventral prostate, and 0.5ug of total RNA from pooled 35-day coagulating gland (anterior prostate ) was combined and used in 1st strand cDNA synthesis. 2nd strand cDNA was generated by 21 cycles of PCR. One prominent band from the double stranded cDNA was removed from the gel and the remaining cDNA was gel isolated. This subtracted cDNA was digested with SfiI and ligated into the lambdaTriplEx2 phagemid. Phagemids were converted to pTriplEx2 plasmids in the BM25.8 e-coli strain. Created from pooled prostate from five 35 day old male C57BL6 mice by Colin Pritchard.

text: prostate

text: Mus musculus

text: SfiI

text: SfiI

text: Male

text: Equal amounts of pooled ventral prostate, dorsolateral prostate, and coagulating gland (anterior prostate) from five 35 day old male mice, subtracted

text: FH DAY35S

text: plasmid (ampicillin resistant)


text: Fred Hutchinson Cancer Research Center

characteristic: INSDC first public

text: live

text: Generic

text: Library was constructed using Clontechs SMART cDNA library construction kit. 1ug of total RNA from pooled dorsolateral prostates was used in 1st strand cDNA synthesis. 2nd strand cDNA was generated by 18 cycles of PCR, digested with SfiI and ligated into the lambdaTriplEx2 phagemid. Phagemids were converted to pTriplEx2 plasmids in the BM25.8 e-coli strain. Created from pooled dorsolateral prostate from 3 month old male mice by Colin Pritchard.

text: prostate

text: Mus musculus

text: SfiI

text: SfiI

text: Male

text: Pooled dorsolateral prostates

text: FH DLP01

text: plasmid (ampicillin resistant)


text: Fred Hutchinson Cancer Research Center

characteristic: INSDC last update

text: live

text: Generic

text: Library was constructed using Clontechs SMART cDNA library construction kit. 0.5ug of total RNA from pooled 14-month dorsolateral prostate, 0.5ug of total RNA from pooled 14-month ventral prostate, and 0.5ug of total RNA from pooled 14 month coagulating gland (anterior prostate) was DNAseI treated, combined and used in 1st strand cDNA synthesis. The RNA from the coagulating gland was partially degraded. 2nd strand cDNA was generated by 23 cycles of PCR, digested with SfiI and ligated into the lambdaTriplEx2 phagemid. Phagemids were converted to pTriplEx2 plasmids in the BM25.8 e-coli strain. Created from pooled prostate from 14 month old male C57BL6 mice by Colin Pritchard.

text: prostate

text: Mus musculus

text: SfiI

text: SfiI

text: Male

text: Equal amounts of pooled ventral prostate, dorsolateral prostate, and coagulating gland (anterior prostate)

text: FH MONTH14

text: plasmid (ampicillin resistant)


text: Fred Hutchinson Cancer Research Center

text: live

characteristic: INSDC status

text: Generic

text: Library was constructed using Clontechs SMART cDNA library construction kit. 0.1ug of total RNA from pooled 2-day dorsolateral prostate, 0.1ug of total RNA from pooled 2-day ventral prostate, and 0.1ug of total RNA from pooled 2-day coagulating gland (anterior prostate) was combined and used in 1st strand cDNA synthesis. 2nd strand cDNA was generated by 23 cycles of PCR, digested with SfiI and ligated into the lambdaTriplEx2 phagemid. Phagemids were converted to pTriplEx2 plasmids in the BM25.8 e-coli strain. Created from pooled prostate from five two day old male C57BL6 mice by Colin Pritchard.

text: prostate

text: Mus musculus

text: SfiI

text: SfiI

text: Male

text: FH NEONATAL

text: plasmid (ampicillin resistant)


text: Fred Hutchinson Cancer Research Center

text: live

text: Generic

characteristic: NCBI submission model

text: Library was constructed using Clontechs SMART cDNA library construction kit. 1ug of total RNA from pooled urogenital sinuses was used in 1st strand cDNA synthesis. 2nd strand cDNA was generated by 18 cycles of PCR, digested with SfiI and ligated into the lambdaTriplEx2 phagemid. Phagemids were converted to pTriplEx2 plasmids in the BM25.8 e-coli strain. Created from pooled urogenital sinuses from E16 mouse embryos (male and female) by Colin Pritchard.

text: prostate

text: Mus musculus

text: SfiI

text: SfiI

text: Male and Female

text: Pooled urogenital sinuses

text: FH UGS01

text: plasmid (ampicillin resistant)


text: Fred Hutchinson Cancer Research Center

text: live

text: Generic

characteristic: NCBI submission package

text: Library was constructed using Clontechs SMART cDNA library construction kit. 1ug of total RNA from pooled ventral prostates was used in 1st strand cDNA synthesis. 2nd strand cDNA was generated by 18 cycles of PCR, digested with SfiI and ligated into the lambdaTriplEx2 phagemid. Phagemids were converted to pTriplEx2 plasmids in the BM25.8 e-coli strain. Created from pooled ventral prostates from 3 month old male C57BL6 mice by Colin Pritchard.

text: prostate

text: Mus musculus

text: SfiI

text: SfiI

text: Male

text: pooled ventral prostate

text: FH VP01

text: plasmid (ampicillin resistant)


text: Fred Hutchinson Cancer Research Center

text: live

text: Generic

text: prostate

text: Mus musculus

text: SfiI

text: SfiI

text: Male

text: pooled ventral prostate

text: FH VP02

text: plasmid (ampicillin resistant)


text: Fred Hutchinson Cancer Research Center

text: live

text: Generic

text: Library was constructed using Clontechs SMART cDNA library construction kit. 0.5ug of total RNA from pooled coagulating glands, ventral prostate, and dorsolateral prostate was used in 1st strand cDNA synthesis. 2nd strand cDNA was generated by 22 cycles of PCR, digested with SfiI and ligated into the lambdaTriplEx2 phagemid. Phagemids were converted to pTriplEx2 plasmids in the BM25.8 e-coli strain. Created from pooled mouse prostate from ten adult male mice by Colin Pritchard.

text: prostate

text: Mus musculus

text: SfiI

text: SfiI

text: Male

text: Pooled coagulating glands (anterior prostate), ventral prostate and dorsolateral prostate from ten adult male mice

text: FH WT129lig05

text: plasmid (ampicillin resistant)


text: Fred Hutchinson Cancer Research Center

text: live

text: Generic

text: Library was constructed using Clontech's SMART cDNA library construction kit. 0.5ug of total RNA from pooled coagulating glands, ventral prostate, and dorsolateral prostate was used in 1st strand cDNA synthesis. 2nd strand cDNA was generated by 22 cycles of PCR, digested with SfiI and ligated into the lambdaTriplEx2 phagemid. Phagemids were converted to pTriplEx2 plasmids in the BM25.8 e-coli strain. Created from pooled mouse prostate from ten adult male mice by Colin Pritchard.

text: prostate

text: Mus musculus

text: SfiI

text: SfiI

text: Male

text: FH WT129lig15

text: plasmid (ampicillin resistant)


text: Advanced Technologies (Cambridge) Ltd

text: live

text: Generic

characteristic: cultivar

text: Germination

characteristic: dev-stage

text: Nicotiana tabacum

text: Seed


text: University of Antwerp

text: live

text: Generic

text: Daphnia magna

characteristic: organism

text: whole body

text: Daphnia magna reproduction related cDNA library


text: USDA, Agricultural Research Service

text: live

text: Generic

text: R16 and M35-1 genotypes

text: Using DDRT-PCR, transcripts upregulated under terminal drought conditions and in response to salicylic acid treatment were cloned. Total RNA extracted from leaf tissue was amplified using RNA Imaging, mRNA differential display system, GenHunter Co., Brookline, MA, USA, per manufacturers protocol.

text: Anthesis

text: Sorghum bicolor

text: Leaf

text: DDRT-PCR ESTs

text: Plasmid

text: pGEM-T Easy vector


text: Institut National de la Recherche Agronomique

text: live

text: Generic

text: The cDNA library was made from pooled mRNAs extracted from midguts of larvae fed artificial diet supplemented with various natural products and xenobiotics

text: 3rd instar larvae

text: midgut

characteristic: organ

text: Spodoptera frugiperda

text: Spodoptera frugiperda midgut cDNA library

text: Plasmid

text: pSport I


text: Agricultural Research Institute

text: live

text: Generic

text: SNBC 61

text: ESTs are isolated from brown planthopper resistant variety after 24 hours of brown planthopper feeding. RDA (Representational Difference Analysis) library made with tester sample, brown planthopper fed resistant rice variety; SNBC 61, and driver sample, susceptible variety; Nagdongbyeo after 48 hrs of brown planthopper feeding

text: Three weeks after sowing

text: E.coli

text: Oryza sativa

text: Brown planthopper resistant rice EST


text: live

text: Generic

text: Non-normalized oligo dT primed cDNA library using poly A+ RNA from mixed embryonic stages of Nematostella vectensis. Cloned directionally into pSPORT 6.1 (Invitrogen). Ligated in NotI, MluI/SalI. (MluI is introduced upon ligation of cDNA insert)

text: mixed stages from unfertilized eggs to primary polyps

text: Nematostella vectensis

text: NotI

text: SalI

text: not determined

characteristic: sex

text: whole embryos

text: Nematostella vectensis non-normalized cDNA library 248

text: plasmid


text: live

text: Generic

text: Normalized oligo dT primed cDNA library using poly A+ RNA from mixed embryonic stages of Nematostella vectensis. Cloned directionally into pBluescript II SK(+). Ligated in NotI, EcoRI

text: Nematostella vectensis

text: NotI

text: EcoRI

text: hermaphrodite

text: whole embryos

characteristic: tissue-type

text: Nematostella vectensis normalized cDNA library 327

text: plasmid

text: pBluescript II SK(+)


text: University of Texas at Austin

text: live

text: Generic

text: Vegetative

characteristic: cell type

text: mRNA was isolated from late log-phase axenic cultures at various times during the light and dark photoperiod phases, and pooled. Directional cloning of cDNA was such that the poly-A(+) tail is at the XhoI end of the insert; the 5' end of the cDNA is at the EcoRI end. Part or all of the EcoRI linker sequence (GGCACGAGG) may be present at the 5' end of the EST sequences.

text: E. coli

text: Prymnesium parvum

text: EcoRI (5' end)

text: XhoI (3' end)

text: Asexual

text: UTEX 2797

characteristic: strain

text: Haptophyte (alga)

characteristic: title

text: Plasmid

text: pBLUESCRIPT II XR (Stratagene)


text: Osmania University

text: live

text: Generic

text: pigeonpea plants of 4 weeks old were subjected to water stress. Total RNA was isolated from leaf tissues. Subtracted cDNA library was constructed.

characteristic: description

text: four week old plants

text: Escherichia coli

characteristic: lab-host

text: leaf

text: Cajanus cajan

text: hermophrodite

text: leaf tissue

text: Pigeonpea water stress induced cDNA library

text: Phage

text: Uni-ZAP XR

characteristic: vector


characteristic: External Id

text: Norwegian School of Veterinary Science

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: Pre-smolt

text: Salmo salar

text: Gills

text: Atlantic salmon gills SSH cDNA library

text: Plasmid

text: pGEM-T Easy


text: Norwegian School of Veterinary Science

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Pre-smolt

text: Salmo salar

text: Gills

text: Atlantic salmon intestine SSH cDNA library

text: Plasmid

text: pGEM-T Easy


text: University of Nevada

text: live

text: Generic

characteristic: NCBI submission package

text: Bradyzoite developmental stage

characteristic: dev-stage

text: PK13 porcine kidney epithelial cells

text: Toxoplasma gondii

text: EcoRI

text: XhoI

text: Toxoplasma gondii Bradyzoite Library

text: pBluescript SK


text: University of Nevada

text: live

text: Generic

text: Tachyzoite developmental stage

text: PK13 porcine kidney epithelial cells

text: Toxoplasma gondii

characteristic: organism

text: EcoRI

text: XhoI

text: Toxoplasma gondii Tachyzoite Library

text: pBluescript SK


text: USDA/ARS

text: live

text: Generic

text: Tissue: Seasonal crown buds from outdoor plants, whole plant tissue (leaf, stem, flowers, seeds, shoots, galls, root and crown buds) from outdoor plants, cold acclimated tissues (leaf, stem, and crown buds), crown and root buds from decapitated greenhouse p ants, and seedlings. The library was constructed and normalized as described by Bonaldo, M.F., Lennon, G. and Soares, M.B. (1996), Genome Research 6(9): 791-806. An identiying tag was added at the 3'during cDNA synthesis: insertAAAAAAAAAAAAAAAAAATGCGT.

text: Euphorbia esula

text: EcoRI(5' side of insert)

text: NotI (3' side of insert)

text: CV03-normalized library

text: Plasmid

text: pBS II SK(+)


text: USDA/ARS

text: live

text: Generic

text: Tissue: roots, leaves, mature leaves, buds, stems and flower buds from a) control, b) cold acclimated, c) drought and post drought stressed and d) heat shocked plants The library was constructed and normalized as described by Bonaldo, M.F., Lennon, G. and Soares, M.B. (1996), Genome Research 6(9): 791-806. An identiying tag was added at the 3'during cDNA synthesis: insertAAAAAAAAAAAAAAAAAATCGGT.

text: Cirsium arvense

text: EcoRI (5' side of insert)

text: NotI (3' side of insert)

text: CV04-normalized library

text: Plasmid

text: pBS II SK(+)


text: Michigan State University

text: live

text: Generic

text: Bing/Gisela5 or Bing/Gisela6

characteristic: cultivar

text: ve samples were excised from the cDNA-AFLP gel and amplified by PCR. The PCR product was cloned in the pGEM-T Easy vector and sequenced with the M13 forward primer.

text: Stem

characteristic: organ

text: Prunus avium x P. cerasus x P. canescens

text: Xylem, Bark

text: Cherry differentially expressed


text: Washington University School of Medicine

text: live

text: Generic

text: GC10 cells

text: Sarcocystis falcatula

text: SfiI B

text: SfiI A

text: Phagemid

text: pDNR-LIB


text: University of Georgia

text: live

text: Generic

text: This expressed sequence tag comes from a clone isolated from a suppression subtraction hybridization (SSH) library constructed using cDNA from reproductive larvae as driver and cDNA from soldier larvae as tester. A SMART cDNA synthesis kit (Clontech) was used to generate sufficient quantities of cDNA for the subtraction.

text: Copidosoma floridanum

text: Reproductive larvae-specific SSH library

text: Plasmid

text: pCRII-TOPO (Invitrogen)


text: University of Georgia

text: live

text: Generic

text: Copidosoma floridanum

text: Soldier larvae-specific SSH library

text: Plasmid

text: pCRII-TOPO (Invitrogen)


text: National Institute of Biomedical Innovation

text: live

text: Generic

text: 1st strand cDNA was primed with an oligo(dT) primer [ATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded cDNA was synthesized using specific 5' and 3' primers and amplified by PCR. The PCR product was digested with SfiI and size selection was performed to exclude fragments <1.5kb.The SfiI-digested PCR product was cloned into distinct DraIII sites of pME18S-FL3. XhoI sites just outside the DraIII sites can be used to isolate the cDNA insert. Libraries were constructed by oligo-capping method.

text: adult

text: Macaca fascicularis

text: DraIII (CACTGTGTG)

text: DraIII (CACCATGTG)

text: male

characteristic: sex

text: brain stem

characteristic: tissue-type

text: macaque brain cDNA library QbsB

text: pME18S-FL3 (Acc.No. AB009864)


text: live

text: Generic

text: adult

text: Macaca fascicularis

text: DraIII (CACTGTGTG)

text: DraIII (CACCATGTG)

text: male

text: brain stem

text: macaque brain cDNA library QbsA

characteristic: title

text: pME18S-FL3 (Acc.No. AB009864)


text: live

text: Generic

characteristic: description

text: adult

text: Macaca fascicularis

text: DraIII (CACTGTGTG)

text: DraIII (CACCATGTG)

text: male

text: cerebellum cortex

text: macaque brain cDNA library QccE

text: pME18S-FL3 (Acc.No. AB009864)


text: live

text: Generic

text: adult

characteristic: lab-host

text: Macaca fascicularis

text: DraIII (CACTGTGTG)

text: DraIII (CACCATGTG)

text: male

text: brain frontal lobe

text: macaque brain cDNA library QflA

text: pME18S-FL3 (Acc.No. AB009864)


text: live

text: Generic

text: adult

text: Macaca fascicularis

text: DraIII (CACTGTGTG)

text: DraIII (CACCATGTG)

text: male

text: medulla oblongata

text: macaque brain cDNA library QmoA

text: pME18S-FL3 (Acc.No. AB009864)

characteristic: vector


characteristic: External Id

text: live

text: Generic

text: adult

text: Macaca fascicularis

text: DraIII (CACTGTGTG)

text: DraIII (CACCATGTG)

text: male

text: brain parietal lobe

text: macaque brain cDNA library QnpA

text: pME18S-FL3 (Acc.No. AB009864)


characteristic: INSDC center name

text: live

text: Generic

text: adult

text: Macaca fascicularis

text: DraIII (CACTGTGTG)

text: DraIII (CACCATGTG)

text: male

text: brain occipital lobe

text: macaque brain cDNA library QorA

text: pME18S-FL3 (Acc.No. AB009864)


characteristic: INSDC first public

text: live

text: Generic

text: adult

text: Macaca fascicularis

text: DraIII (CACTGTGTG)

text: DraIII (CACCATGTG)

text: male

text: brain temporal lobe

text: macaque brain cDNA library QtrA

text: pME18S-FL3 (Acc.No. AB009864)


characteristic: INSDC last update

text: live

text: Generic

text: adult

text: Macaca fascicularis

text: DraIII (CACTGTGTG)

text: DraIII (CACCATGTG)

text: male

text: testis

text: macaque testis cDNA library QtsA

text: pME18S-FL3 (Acc.No. AB009864)


text: live

characteristic: INSDC status

text: Generic

text: Viola baoshanensis

text: root

text: Cd-induced root cDNA subtractive library in Viola baoshanensis


text: Royal Institute of Technology, KTH

text: live

text: Generic

characteristic: NCBI submission model

text: CDNA made from mRNA extracted from midgut tissues and cloned into the vector according to Creator SMARt cDNA library construction kit (Clontech Laboratories).

text: stage four larval instar

text: Midgut

text: Chironomus tentans

text: Several from midgut

text: C. tentans, embryo midgut

text: Plasmid

text: pDNR-LIB


text: Cotton Research Institute, Chinese Academy of Agricultural Sciences

text: live

text: Generic

characteristic: NCBI submission package

text: genotype: AD

text: Gossypium hirsutum

characteristic: strain

text: embryoid callus

text: Gossypium hirsutum cv. TM-1 embryoid callus


text: Trinity University

text: live

text: Generic

text: 23 day old

characteristic: dev-stage

text: Rattus norvegicus

characteristic: organism

text: breed W

text: Rattus norvegicus 23 day old


text: USDA/ARS

text: live

text: Generic

text: Rainbow trout embryos were collected post fertilization at cleavage day 2, gastrulation day 7, organogenesis day 16, hatch day 31, and yolk sac absorption day 42 for subtraction with oocyte samples total embryo

text: Oncorhynchus mykiss

text: EMB


text: Mount Desert Island Biological Laboratory

text: live

text: Generic

text: Squalus acanthias embryo-derived cell line SAE

characteristic: cell-line

text: Continuously proliferating cell cultures were derived from an embryonic dogfish shark. Total RNA was extracted from 500,000,000 cells using TRIzol reagent followed by mRNA isolation. The primary cDNA library was directionally cloned and normalized by Invitrogen using subtractive hybridization at two different Cot values, resulting in a 280-fold reduction in the abundance of beta-actin sequences. Average insert size was 2.0 kb. Inserts were single-pass sequenced from the 5' end by Rexagen. Traces were trimmed and prepared for submission to dbEST using trace2dbest software (Parkinson, Anthony, and Blaxter, unpublished). This expressed sequence tag project was supported by NIH Grant Number 1 RO1 019732-01 from the National Center for Research Resources (D.W. Barnes, PI), the Woodcock Foundation (D.W. Barnes and C.J. Bayne, PI) and P20-RR-16463 from the INBRE Program of the National Center for Research Resources (P.H. Hand, PI).

text: Embryo with external yolk sac

text: Squalus acanthias

text: Gulf of Maine (North Atlantic)

text: Dogfish Shark Embryo-derived Cell Line SAE, Normalized

text: CMV Sport 6.1


text: Hanyang University

text: live

text: Generic

text: Mixture of Immature & Mature cyst cell

text: SOLR strain

text: Haematococcus lacustris

text: EcoR I

text: Xho I

text: H. pluvialis cDNA Lamda ZAP library

text: pBluescript SK(-)

text: Lamda Zap vector


text: Brooms Barn Research

text: live

text: Generic

text: challenged with Polymyxa graminis zoospores

text: cotyledon to first true leaf stage

text: Beta vulgaris

text: roots

text: Beta vulgaris roots cotyledon to first true leaf stage


text: The Institute for Genomic Research (TIGR; www.tigr.org); University of Notre Dame, Department of Biological Sciences

text: live

text: Generic

text: Adult

text: Aedes aegypti

text: Female

characteristic: sex

text: Pooled eggs, pupae, newly emerged adults, 5-day old adults

text: Aedes aegypti full length cDNA library, 475-850 bp


text: live

text: Generic

text: Adult

text: Aedes aegypti

text: Female

characteristic: tissue-type

text: Aedes aegypti full length cDNA library, 900-1400 bp


text: live

text: Generic

text: Adult

text: Aedes aegypti

text: Female

text: re-sequenced clones from a mixture of (Brugia/Plasmodium/Dengue) infected and fat body cDNA libraries

text: Aedes re-sequencing

characteristic: title


text: live

text: Generic

text: Adult

text: Aedes aegypti

text: Female

text: fat body tissues from adult females at 12 h intervals from 0 h post-eclosion to 96 h post blood feeding

text: Aedes aegypti - Fat Bodies Normalized (NAFFB2)


text: live

text: Generic

text: Adult

text: Aedes aegypti

text: Female

text: adult females at 24 h, 3 days and 12 days post feeding on a Brugia malayi infected blood meal

text: Aedes aegypti infected with Brugia Malayi


text: live

text: Generic

text: MOYO-R strain, adult females at 24 h, 3 days, 8 days, and 12 days post feeding on a Plasmodium gallinaceum infected blood meal RED strain, adult females at 24 h, 3 days and 8 days post feeding on a Plasmodium gallinaceum infected blood meal

characteristic: description

text: Adult

text: Aedes aegypti

text: Female

text: Aedes aegypti infected with Plasmodium gallinaceum


text: live

text: Generic

text: Adult

text: Aedes aegypti

text: Female

text: Ibo-11 strain and DS3 strains: adult females at 24 h, 3 days, 7 days and 14 days post feeding on a Dengue-2 JAM1409 infected blood meal

text: Aedes aegypti infected with Dengue virus Pool library


text: Washington University School of Medicine

text: live

text: Generic

text: a.1st strand cDNA was primed with a Not I primer-adapter (5'- pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3') b.Double-stranded cDNA was ligated to Sal I adapter, digested with Not I and cloned into the pSPORT 1-vector pre-cut with Not I and Sal I. c.The ligation mix was transformed into DH10B cells. d.The cells were grown in SOC = 5g yeast, 20g tryptone, 0.5 g NaCl, 10 mM MgSO4, 10 mM MgCl, 0.2% glucose / Liter, (no antibiotic). e.DMSO was added to a final conc. of 10% as a cryoprotectant.and frozen. f.The titre before freezing was determined as ~10000 / 100 ul. Assuming a 10% loss upon freezing, the titre is probably ~ 9000/ 100 ul. g.4 tubes each containing ~ 9000 clones/ul [ = total of ~ 36,000] are enclosed. h.The frequency of vectors containing inserts is 100% as determined by digestion check after picking 10 clones, miniprep and subsequent digestion with Not I and Sal I. i.A low level of 32P was used in the cDNA synthesis procedure. The level measured by holding a Geiger Counter next to a tube was background.

text: embryos of different stages

text: Electro Max DH10B cells

characteristic: lab-host

text: Hydra vulgaris

text: Not I

text: Sal I

text: AEP

text: plasmid


text: live

text: Generic

text: a.1st strand cDNA was primed with a Not I primer-adapter (5' - pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3') b.Double-stranded cDNA was ligated to Sal I adapter, digested with Not I and cloned into the pSPORT 1-vector pre-cut with Not I and Sal I. c.The ligation mix was transformed into DH10B cells. d.The cells were grown in SOC = 5g yeast, 20g tryptone, 0.5 g NaCl, 10 mM MgSO4, 10 mM MgCl, 0.2% glucose / Liter, (no antibiotic). e.DMSO was added to a final conc. of 10% as a cryoprotectant.and frozen f.The titre before freezing was determined as ~10000/ 100 ul. Assuming a 10% loss upon freezing, the titre is probably ~ 9000/ 100 ul. g.4 tubes each containing ~ 9000 clones/ul [ = total of ~ 45,000] are enclosed. h.The frequency of vectors containing inserts is 90% as determined by digestion check after picking 20 clones, miniprep and subsequent digestion with Not I and Sal I. i.A low level of 32P was used in the cDNA synthesis procedure. The level measured by holding a Geiger Counter next to a tube was background.

text: whole animal and embryo

text: Electro Max DH10B cells

text: Podocoryna carnea

text: Not I

text: Sal I

text: P3 and W

text: plasmid

characteristic: vector


characteristic: External Id

text: Texas A&M University

text: live

text: Generic

text: Calcutta 4

characteristic: cultivar

text: Musa acuminata subsp. burmannicoides

text: leaf tissue treated with Mycosphaerella fijiensis extracts

text: Musa acuminata subsp. burmannicoides cv. Calcutta 4 library


text: National Eye Institute

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: ogen, Carlsbad, CA) at Bioserve Biotechnology (Laurel MD) following the protocols of the SuperScript Plasmid System, full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried ut using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. cDNA was cloned in Not I/Sal I sites. EST analysis was performed at the NIH Intramural Sequencing Center (NISC). Analyzed data available through http://neibank.nei.nih.gov.

text: adult

text: Eye

characteristic: organ

text: Rattus norvegicus

text: Wistar

text: eye iridocorneal angle

text: Rat eye iridocorneal angle. Unnormalized (gw,gx)

text: Plasmid


text: University of Florida

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Hydrilla verticillata

text: Hydrilla verticillata cDNAs isolated by differential display RT-PCR


text: Center for Genetic Engineering and Biotechnology

text: live

text: Generic

characteristic: NCBI submission package

text: Nicotiana megalosiphon

text: Leaf

text: SSH cDNA Library


text: Uppsala University

text: live

text: Generic

text: representational difference analysis of White Leghorn female liver subtracted from Red Junglefowl female liver

text: adult

characteristic: dev-stage

text: Gallus gallus

characteristic: organism

text: female

text: liver


text: Hunan Agricultural University

text: live

text: Generic

text: piglet

text: Sus scrofa

text: small intestine

text: piglet small intestine


text: US EPA

text: live

text: Generic

text: Made using the Creator SMART cDNA library kit from Clontech/Bioscience

text: Daphnia magna

text: D. magna, normal

text: Plasmid

text: pDNR-LIB, Clontech/Bioscience


text: Shandong Agriculture University

text: live

text: Generic

text: shannong No.6

text: Equal quantities of RNA from different senescence cotyledons stages were combined prior to mRNA isolation. In the cDNA subtraction procedure, the reference cDNA was from fully expansion mature cotyledons.

text: 22, 26, and 30 days after imbibition

text: Gossypium hirsutum

text: senescence cotyledon

text: A suppression subtractive hybridization cDNA library from developmental senescence cotyledons

text: Plasmid

text: pGEM-T easy vector


text: University of New Hampshire

text: live

text: Generic

text: 'Yellow Wonder'

text: For more details on library preparation and sequence analysis see http://www.genome.clemson.edu/gdr/projects/fragaria/davist/davisf/index.shtml

text: E. coli

text: Fragaria vesca subsp. vesca

text: SFI I

text: unopened flower buds

text: Fragaria vesca 'Yellow Wonder' flower buds.

text: Plasmid

text: pDNR-LIB


text: University of Florence, Italy

text: live

text: Generic

text: in vitro plantlets infested with Cacopsylla pyri

text: Pyrus communis x Pyrus ussuriensis

text: Subtractive cDNA library of Cacopsylla pyri-infested versus non-infested pear plants

text: Plasmid

text: pGEM-T Easy


text: University of Florence, Italy

text: live

text: Generic

text: William

text: Pyrus communis

text: Subtractive cDNA library of Cacopsylla pyri-infested versus non-infested susceptible pear plants

text: Plasmid

text: pGEM-T Easy


text: USDA/ARS

text: live

text: Generic

text: The library was constructed and normalized as described by Bonaldo, M.F., Lennon, G. and Soares, M.B. (1996), Genome Research 6(9): 791-806. An identifying tag was added at the 3'during cDNA synthesis: insertAAAAAAAAAAAAAAAAAATCGGA.

text: Manihot esculenta

text: EcoRI(5' side of insert)

text: NotI (3' side of insert)

text: Mature leaf and petiole, young leaf and apical meristem, root, tuber and tuber peel, young leaf and apical meristem midnight

text: CV01-normalized library

text: Plasmid

text: pBS II SK(+)


text: USDA/ARS

text: live

text: Generic

text: The library was constructed and normalized as described by Bonaldo, M.F., Lennon, G. and Soares, M.B. (1996), Genome Research 6(9): 791-806. An identifying tag was added at the 3'during cDNA synthesis: insertAAAAAAAAAAAAAAAAAATGCGA.

text: Manihot esculenta

text: EcoRI(5' side of insert)

text: NotI (3' side of insert)

text: Young leaf and apical meristem, mature leaf and petiole, root, tuber and tuber peel from water stressed plants

text: CV02-normalized library

text: Plasmid

text: pBS II SK(+)


text: National Institute on Deafness and Other Communication Disorders

text: live

text: Generic

text: Human fetal cochlea cDNA library normalized to brain, testis, kidney, uterus, and fibroblast according to Clontech PCR-Select cDNA Subtraction method.

text: Fetal

text: ear

text: Homo sapiens

text: EcoRI

text: EcoRI

text: cochlea

text: Human Fetal Cochlea cDNA Library

text: Plasmid


text: Michigan Technological University, School of Forest Resources & Environmental Science

text: live

text: Generic

text: Clone: NUL. Leaves and stems (LPI 1-5) harvested from control plants at different dinural points (noon or midnight), and systemic levaes (LPI 1-5) harvested 48-hr after SA treatment were used for mRNA isolation. mRNA from these sources were pooled for cDNA library construction and normalization according to Gruber et al. (1993) Focus 15: 59-65.

text: 2-month-old hydroponic plants

text: E. coli DH10B, T1 phage resistant

text: Populus fremontii x Populus angustifolia

text: StuI (custom-generated at SalI s

text: NotI,3'-end of cDNA

text: NUL


text: Research Institute, Heilongjiang Academy of Agricultural Sciences

text: live

text: Generic

text: Harbin xiaoheidou

text: Root

text: Glycine max

text: cDNA sequences from soybean roots induced by pathogen (Heterodera glycines) (Liu J)


text: USDA-ARS Western Regional Research Center

text: live

text: Generic

text: Library sequences were extracted from ABI 3730XL tracefiles. Quality Read Lengths from sequences were extracted with Phred program. File trimming based on sequence mark-up done with cross_match program, minor vector sequences remained. Quality files and additional information available from project site at: http://wheat.pw.usda.gov/bEST

text: callus

text: Escherichia coli DH5alpha-FT

text: Brachypodium distachyon

text: SalI

text: NotI

text: callus

text: Brachypodium distachyon callus EST library

text: plasmid


text: live

text: Generic

text: mature

text: Escherichia coli OmniMax 2-T1

text: Brachypodium distachyon

text: SalI

text: NotI

text: leaf

text: Brachypodium distachyon leaf EST library

text: plasmid


text: live

text: Generic

text: hydroponically grown

text: Escherichia coli OmniMax 2-T1

text: Brachypodium distachyon

text: SalI

text: NotI

text: root

text: Brachypodium distachyon root EST library

text: plasmid


text: live

text: Generic

text: mature

text: Escherichia coli OmniMax 2-T1

text: Brachypodium distachyon

text: SalI

text: NotI

text: stem plus sheath

text: Brachypodium distachyon stem plus sheath EST library

text: plasmid


text: live

text: Generic

text: mature

text: Escherichia coli OmniMax 2-T1

text: Brachypodium distachyon

text: SalI

text: NotI

text: developing spike

text: Brachypodium distachyon developing spike EST library

text: plasmid


text: National Center of Excellence in Molecular Biology, University of the Punjab,

text: live

text: Generic

text: FDH 786

text: CDNA was made from cotton RNA. The fragment was amplified by RACE PCR and TA cloned in vector PCR 2.1. It seems to be a homologue of fructose bisphosphate aldolase gene of rice and arabidopsis.

text: 4 leaf seedling stage

text: leaf

text: Gossypium arboreum

text: Hermaphrodite

characteristic: sex

text: total leaf tissue

text: Cotton cDNA library

text: Plasmid

text: PCR 2.1


text: Iowa State University

text: live

text: Generic

text: Zea mays

text: UGI-Reseq


text: National Institute of Agricultural Biotechnology

text: live

text: Generic

text: Solanum tuberosum

text: EcoRI

text: XhoI

text: Solanum tuberosum cDNA library

text: pBK-CMV


text: Kyoto University

text: live

text: Generic

text: Ciona intestinalis

text: Yutaka Satou unpublished library (cicx)


text: The University of Shiga Prefecture

text: live

text: Generic

text: Alexandrium catenella

characteristic: strain

text: Alexandrium catenella OF72


text: Shimane University

text: live

text: Generic

text: tobacco BY-2

characteristic: cell-line

text: Bright Yellow No.2

text: stationary phase

text: Nicotiana tabacum

text: Nicotiana tabacum tobacco BY-2

characteristic: title


text: USDA-ARS

text: live

text: Generic

text: F1010, GP-131, PI 535818

text: Sugar beets were grown in soil for 2-3 weeks. Soil was removed and seedlings were infested with sugar beet root maggots for 24hr or 48 hr. Roots with < 1cm hypocotyl were harvested and used for cDNA synthesis

characteristic: description

text: 17 days post germination

text: Beta vulgaris

text: root/hypocotyl

characteristic: tissue-type

text: F1010 infested vs F1010 uninfested

text: pCR2.1-TOPO (Invitrogen)


characteristic: External Id

text: USDA-ARS

text: live

text: Generic

text: F1016, GP-207, PI608437

text: 17 days post germination

text: Beta vulgaris

text: root/hypocotyl

text: F1016 infested vs F1016 uninfested

text: pCR2.1-TOPO (Invitrogen)

characteristic: vector


text: USDA-ARS

characteristic: INSDC center name

text: live

text: Generic

text: F1010 (PI 535818) and F1016 (PI608437)

characteristic: cultivar

text: 17 days post germination

text: Beta vulgaris

text: root/hypocotyl

text: F1010 vs F1016

text: pCR2.1-TOPO (Invitrogen)


text: The Institute of Physical and Chemical Research (RIKEN)

characteristic: INSDC first public

text: live

text: Generic

text: 10 days embryo

text: Mus musculus

text: RIKEN full-length enriched mouse cDNA library, C57BL/6J 10 days embryo


characteristic: INSDC last update

text: live

text: Generic

text: 10, 11 days embryo

text: Mus musculus

text: RIKEN full-length enriched mouse cDNA library, C57BL/6J 10, 11 days embryo


text: live

characteristic: INSDC status

text: Generic

text: 11 days embryo

text: Mus musculus

text: RIKEN full-length enriched mouse cDNA library, C57BL/6J 11 days embryo


text: live

text: Generic

characteristic: NCBI submission model

text: 7 days embryo

text: Mus musculus

text: RIKEN full-length enriched mouse cDNA library, C57BL/6J 7 days embryo


text: Rajiv Gandhi Centre for Biotechnology

text: live

text: Generic

characteristic: NCBI submission package

text: Rio-de-janeiro

text: Zingiber officinale

text: leaf

text: Zingiber officinale cv. Rio-de-janeiro leaf


text: Rajiv Gandhi Centre for Biotechnology

text: live

text: Generic

text: Varada

text: Zingiber officinale

text: leaf

text: Zingiber officinale cv. Varada leaf


text: Rajiv Gandhi Centre for Biotechnology

text: live

text: Generic

text: Zingiber zerumbet

text: leaf

text: Zingiber zerumbet leaf


text: Rajiv Gandhi Centre for Biotechnology

text: live

text: Generic

text: Wild

text: Zingiber officinale

text: leaf

text: Zingiber officinale Wild leaf


text: Harbin Institute of Technology

text: live

text: Generic

text: Arcopilus cupreus

text: mycelium

text: Arcopilus cupreus mycelium cDNA library


text: University of the Sunshine Coast

text: live

text: Generic

text: mature

characteristic: dev-stage

text: gonad

characteristic: organ

text: Pinctada maxima

text: male and female

text: P. maxima gonad subtractive library

text: plasmid


text: Ludwig-Maximilians University Muenchen

text: live

text: Generic

text: Subtracted cDNA library; SSH method; enriched for cDNAs up-regulated at estrus compared to diestrus in bovine endometrium

text: estrus (day 0 of the estrous cycle)

text: E. coli SURE

characteristic: lab-host

text: uterus

text: Bos taurus

characteristic: organism

text: BssHII

text: BssHII

text: female

characteristic: sex

text: Simmental

text: endometrium

text: Bovine SSH library; endometrium; estrus; OE3

text: Plasmid

text: derivative of pBSIISK(-)


text: Ludwig-Maximilians University Muenchen

text: live

text: Generic

text: Subtracted cDNA library; SSH method; enriched for cDNAs up-regulated at diestrus compared to estrus in bovine endometrium

text: Diestrus (day 12 of the estrous cycle)

text: E. coli SURE

text: uterus

text: Bos taurus

text: BssHII

text: BssHII

text: female

text: Simmental

characteristic: strain

text: endometrium

text: Bovine SSH library; endometrium; diestrus; DI3

characteristic: title

text: Plasmid

text: derivative of pBSIISK(-)


text: live

text: Generic

text: 13 days embryo

text: Mus musculus

text: RIKEN full-length enriched mouse cDNA library, C57BL/6J 13 days embryo


text: live

text: Generic

text: 18 days embryo

text: Mus musculus

text: RIKEN full-length enriched mouse cDNA library, C57BL/6J 18 days embryo


text: live

text: Generic

text: 6 days embryo

text: Mus musculus

text: RIKEN full-length enriched mouse cDNA library, C57BL/6J 6 days embryo


text: live

text: Generic

text: 8 days embryo

text: Mus musculus

text: RIKEN full-length enriched mouse cDNA library, C57BL/6J 8 days embryo


text: live

text: Generic

text: 14, 17 days embryo

text: Mus musculus

text: head

characteristic: tissue-type

text: RIKEN full-length enriched mouse cDNA library, C57BL/6J head 14, 17 days embryo


characteristic: External Id

text: live

text: Generic

text: 10 day old

text: Mus musculus

text: male

text: pancreas

text: RIKEN full-length enriched mouse cDNA library, C57BL/6J pancreas male 10 day old


text: University of Agricultural Sciences

text: live

text: Generic

text: Finger millet 15-day-old seedlings were subjected to different water stress (20%F.C, 40%F.C, 60%F.C and 100%F.C) by withholding water till the crop plants withstands to the respective stress level. RNA isolated from leaf tissues. Subtracted cDNA library was constructed using TA cloning kit.

characteristic: description

text: Vegetative growth stage

text: Escherichia coli

text: Leaf

text: Eleusine coracana

text: EcoRI

text: EcoRI

text: Hermaphrodite

text: Leaf tissue

text: Finger millet drought stress cDNA library

characteristic: vector


text: Shanghai Institute of Animal Parasitology, Chinese Academy of Agricultural Sciences

characteristic: INSDC center name

text: live

text: Generic

text: Female subtracted cDNA library using cDNA from female cDNA as testers and from male as drivers. Subtractive PCRs were performed using the PCR-select cDNA subtractive kit according to the manufacture's protocol (clontech, palo Alto, CA). PCR products then had been directionally subcloned into the pGEM-T Easy vector (Promega) and transformed into JM109 competent cells (Promega).

text: Adult worm

text: Rabbit

text: Schistosoma japonicum

text: Female

text: Chinese mainland

text: Whole body

text: Female subtracted cDNA library

text: Plasmid

text: pGEM-T Easy


characteristic: INSDC first public

text: live

text: Generic

text: Male subtracted cDNA library using cDNA from male cDNA as testers and from female as drivers. Subtractive PCRs were performed using the PCR-select cDNA subtractive kit according to the manufacture's protocol (clontech, palo Alto, CA). PCR products then had been directionally subcloned into the pGEM-T Easy vector (Promega) and transformed into JM109 competent cells (Promega).

text: Adult worm

text: Rabbit

text: Schistosoma japonicum

text: Male

text: Chinese mainland

text: Whole body

text: Male subtracted cDNA library pGEM-T

text: Plasmid

text: pGEM-T Easy


text: National Eye Institute, NIH

characteristic: INSDC last update

text: live

text: Generic

text: Total corneas were dissected from 40 mouse eyes under a dissection microscope. Total RNA was isolated from the indicated tissues (RNazol; TelTest, Friendswood, TX). Total RNA (300 ug) was used for construction of the library. Poly(A)+RNA was prepared using Dynabeads Oligo (dT)25 (Invitrogen, Carlsbad, CA). The cDNA was synthesized and directionally cloned in the Uni-ZAP XR vector (Stratagene, La Jolla, CA). First strand synthesis was carried out using an oligo(dT) linker-primer containing an Xho I site [5'-(GA)10-ACTAGTCTCGAG(T)18-3']. cDNA was cloned in EcoR I-Xho I sites. In vivo excision releases the pBluescript SK(+/-) phagemid. Templates were sequenced using the Beckman CEQ2000 sequencing kit on a CEQ2000 Genomic Analysis System (Beckman-Coulter, Fullerton, CA). Analyzed data available through http://neibank.nei.nih.gov. NbLib0115: NEI mouse cornea (adult) unnormalized (bn1)

text: adult (6 week)

text: eye

text: Mus musculus

text: total cornea

text: Mouse total cornea. Unnormalized

text: Plasmid

text: pBluescript SK(+/-)


text: National Eye Institute, NIH

text: live

characteristic: INSDC status

text: Generic

text: Cornea endothelium and stroma was dissected from 200 mouse eyes under a dissection microscope. Total RNA was isolated from the indicated tissues (RNazol; TelTest, Friendswood, TX). Total RNA (93.4 ug) was used for construction of the library. Poly(A)+RNA was prepared using Dynabeads Oligo (dT)25 (Invitrogen, Carlsbad, CA). The cDNA was synthesized and directionally cloned in the Uni-ZAP XR vector (Stratagene, La Jolla, CA). First strand synthesis was carried out using an oligo(dT) linker-primer containing an Xho I site [5'-(GA)10-ACTAGTCTCGAG(T)18-3']. cDNA was cloned in EcoR I-Xho I sites. In vivo excision releases the pBluescript SK(+/-) phagemid. Templates were sequenced using the Beckman CEQ2000 sequencing kit on a CEQ2000 Genomic Analysis System (Beckman-Coulter, Fullerton, CA). Analyzed data available through http://neibank.nei.nih.gov. NbLib0116: NEI mouse cornea (adult) unnormalized (bn2) (enriched for endothelium and stroma).

text: adult (6 week)

text: eye

text: Mus musculus

text: Mouse cornea enriched for endothelium and stroma. Unnormalized.

text: Plasmid

text: pBluescript SK(+/-)


text: BioPia

text: live

text: Generic

characteristic: NCBI submission model

text: leaf

text: Panax ginseng

text: Sfi lA

text: Sfi lB

text: Panax ginseng cDNA library


text: Zhong-Shan Medical College, Sun Yat-sen University

text: live

text: Generic

characteristic: NCBI submission package

text: maturity

text: Deinagkistrodon acutus

text: Venom gland

text: venom gland cDNA library

text: Plasmid

text: pBluescriptII


text: Agriculture and Agri-food Canada

text: live

text: Generic

characteristic: cultivar

text: cDNA-AFLP differential TDFs from Phaseolus vulgaris inoculated with Xanthomonas axonopodis pv. Phaseoli, partial CDS.

text: Phaseolus vulgaris

text: leaf

text: cDNA-AFLP TDF from Phaseolus vulgaris


text: The University of Birmingham

text: live

text: Generic

text: Directionally cloned, as described at http://www.genipol.stir.ac.uk/resources.html

text: Immature

characteristic: dev-stage

text: Liver

characteristic: organ

text: Platichthys flesus

text: mixed

text: Flounder IL Induced Liver library

text: pTriplEx2 (BD Biosciences)


text: University of Puerto Rico

text: live

text: Generic

text: Mixed species/subspecies: Heliconius erato petiverana, Heliconius erato erato, Heliconius erato cyrbia, Heliconius himera Tissue: Wing-disk tissue collected from: 5th instar, early prepupal, late prepupal, 24 hours after pupation, precolor pupal stages (i.e. pupae > 48 hour after pupation, but before the development of wing pigmentation), 10 days after pupation

text: Heliconius erato/himera mixed EST library

characteristic: organism

text: Heliconius erato wing disk

text: lambda UniZAP


text: Helix Research Institute

text: live

text: Generic

text: neuroblastoma

characteristic: cell type

characteristic: cell-line

text: Homo sapiens


text: Helix Research Institute

text: live

text: Generic

text: activated T-cells

text: Homo sapiens

characteristic: title


text: Helix Research Institute

text: live

text: Generic

text: Homo sapiens

text: adrenal gland

characteristic: tissue-type


characteristic: External Id

text: Helix Research Institute

text: live

text: Generic

text: normal astrocytes (NHA5732)

text: Homo sapiens


text: Helix Research Institute

text: live

text: Generic

text: adult

text: Homo sapiens

text: breast


text: Helix Research Institute

text: live

text: Generic

text: glioma separated from gliosarcoma

text: Homo sapiens


text: Helix Research Institute

text: live

text: Generic

text: Homo sapiens

text: bladder


text: Helix Research Institute

text: live

text: Generic

text: neuroglioma

text: Homo sapiens


text: Helix Research Institute

text: live

text: Generic

text: Homo sapiens

text: cerebellum


text: Helix Research Institute

text: live

text: Generic

text: Homo sapiens

text: alzheimer cortex


text: Helix Research Institute

text: live

text: Generic

text: Homo sapiens

text: amygdala


text: Helix Research Institute

text: live

text: Generic

text: Homo sapiens

text: brain


text: Helix Research Institute

text: live

text: Generic

text: Homo sapiens

text: caudate nucleus


text: Helix Research Institute

text: live

text: Generic

text: Homo sapiens

text: corpus callosum


text: Helix Research Institute

text: live

text: Generic

text: Homo sapiens

text: hippocampus


text: Helix Research Institute

text: live

text: Generic

text: Homo sapiens

text: substantia nigra


text: Helix Research Institute

text: live

text: Generic

text: activated T-cells

text: primary culture, activated T-cells

characteristic: description

text: Homo sapiens


text: Helix Research Institute

text: live

text: Generic

text: subtracted library (BRALZ - BRAWH)

text: Homo sapiens

text: alzheimer cortex

characteristic: vector


text: Helix Research Institute

characteristic: INSDC center name

text: live

text: Generic

text: Homo sapiens

text: adipose


text: Duke University Medical Center - Department of Neurobiology

characteristic: INSDC first public

text: live

text: Generic

text: The library was constructed as described by Carninci, P. (2003) in DNA Microarrays: A Molecular Cloning Manual, eds. Bowtell, D. & Sambrook, J. (Cold Spring Harbor, NY), pp. 647-670.

text: Adult

text: brain

text: Taeniopygia guttata

text: EcoRI

text: BamHI

text: male

characteristic: sex

text: brain

text: library 33 - normalized (Single directed singing) - male

text: Plasmid


characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Embryonic

text: brain

text: Taeniopygia guttata

text: EcoRI

text: BamHI

text: female

text: brain

text: library 57 - normalized (6 mix pooled embryonic) - female

text: Plasmid


text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Embryonic

text: brain

text: Taeniopygia guttata

text: EcoRI

text: BamHI

text: male

text: brain

text: library 57 - normalized (6 mix pooled embryonic) - male

text: Plasmid


text: live

text: Generic

text: adult and juvenile

characteristic: dev-stage

text: brain

text: Taeniopygia guttata

text: EcoRI

text: BamHI

text: female

text: brain

text: library 58 - normalized (50 mix pooled juveniles + adults) - female

text: Plasmid


text: live

text: Generic

text: adult and juvenile

text: brain

characteristic: organ

text: Taeniopygia guttata

text: EcoRI

text: BamHI

text: male

text: brain

text: library 58 - normalized (50 mix pooled juveniles + adults) - male

text: Plasmid


text: live

text: Generic

text: adult

text: brain

text: Taeniopygia guttata

characteristic: organism

text: EcoRI

text: BamHI

text: male

text: brain

text: library 61 - subtracted (Single male minus single female) - male

text: Plasmid


text: live

text: Generic

text: adult

text: brain

text: Taeniopygia guttata

text: EcoRI

text: BamHI

text: male

text: brain

text: library 62 - subtracted (2 male singers minus single male silent control) - male

text: Plasmid


text: live

text: Generic

text: The library was constructed described by Carninci, P. (2003) in DNA Microarrays: A Molecular Cloning Manual, eds. Bowtell, D. & Sambrook, J. (Cold Spring Harbor, NY), pp. 647-670.

text: juvenile

text: brain

text: Taeniopygia guttata

text: EcoRI

text: BamHI

text: male

text: brain

text: library 63 - subtracted (Single juvenile learning minus single juvenile untutored) - male

text: Plasmid


text: live

text: Generic

text: juvenile

text: brain

text: Taeniopygia guttata

text: EcoRI

text: BamHI

text: male

text: brain

text: library 64 - subtracted (Single juvenile learning minus single male silent control) - male

text: Plasmid


text: Helix Research Institute

text: live

text: Generic

text: Homo sapiens

text: amygdala


text: Helix Research Institute

text: live

text: Generic

text: adult

text: Homo sapiens

text: breast


text: Helix Research Institute

text: live

text: Generic

text: glioma separated from gliosarcoma

characteristic: cell type

text: Homo sapiens


text: Helix Research Institute

text: live

text: Generic

text: Homo sapiens

text: thalamus


text: Helix Research Institute

text: live

text: Generic

text: Homo sapiens

text: colon


text: Helix Research Institute

text: live

text: Generic

text: CD34+ cells after 3-days ODF induction

text: primary culture, CD34+ cells

text: Homo sapiens


text: Helix Research Institute

text: live

text: Generic

text: fetal

text: Homo sapiens

text: brain


text: Helix Research Institute

text: live

text: Generic

text: fetal

text: Homo sapiens

text: heart


text: Helix Research Institute

text: live

text: Generic

text: fetal

text: Homo sapiens

text: lung


text: Helix Research Institute

text: live

text: Generic

text: dermal papilla cells (HDPC)

text: primary culture, dermal papilla cells

text: Homo sapiens


text: Helix Research Institute

text: live

text: Generic

text: Homo sapiens

text: lung


text: Helix Research Institute

text: live

text: Generic

text: neuroblastoma

characteristic: cell-line

text: Homo sapiens


text: Helix Research Institute

text: live

text: Generic

text: normal mesangial cells (NHMC56046-2)

text: primary culture, normal mesangial cells

text: Homo sapiens

characteristic: title


text: Helix Research Institute

text: live

text: Generic

text: normal neural progenitor cells (NHNP5958)

text: primary culture, normal neural progenitor cells

text: Homo sapiens


text: Helix Research Institute

text: live

text: Generic

text: teratocarcinoma

text: mRNA from NT2 neuron after the differentiation of NT2 neuronal precursor cells

text: Homo sapiens


text: Helix Research Institute

text: live

text: Generic

text: Homo sapiens

text: tongue, tumor tissue

characteristic: tissue-type


characteristic: External Id

text: Helix Research Institute

text: live

text: Generic

text: CD34+ cells

text: primary culture, CD34+ cells

text: Homo sapiens

text: cord blood


text: Helix Research Institute

text: live

text: Generic

text: CD34+ cells after 9-days ODF induction

text: primary culture, CD34+ cells

characteristic: description

text: Homo sapiens


text: Helix Research Institute

text: live

text: Generic

text: coronary artery smooth muscle cells (HCASMC)

text: primary culture, coronary artery smooth muscle cells

text: Homo sapiens

characteristic: vector


text: Helix Research Institute

characteristic: INSDC center name

text: live

text: Generic

text: leukemia cell line (myelogenous)

text: Homo sapiens


text: Helix Research Institute

characteristic: INSDC first public

text: live

text: Generic

text: Homo sapiens

text: kidney


text: Helix Research Institute

text: live

text: Generic

text: Homo sapiens

text: subthalamic nucleus


text: Helix Research Institute

text: live

text: Generic

text: fetal

text: Homo sapiens

text: kidney


text: Iowa State University

text: live

text: Generic

text: Zea mays


text: Helix Research Institute

text: live

text: Generic

text: Homo sapiens

text: cord blood


text: Helix Research Institute

text: live

text: Generic

text: mesenchymal stem cells

text: Homo sapiens


text: Helix Research Institute

text: live

text: Generic

text: teratocarcinoma

text: majorly NT2 neuron; mRNA from NT2 neuronal precursor cells treated 2-weeks mitotic inhibitor after 5-weeks retinoic acid (RA) induction.

text: Homo sapiens


text: Helix Research Institute

text: live

text: Generic

text: normal dermal fibroblasts (Neonatal Skin) (NHDF2564)

text: primary culture, normal dermal fibroblasts

text: Homo sapiens


text: Helix Research Institute

text: live

text: Generic

text: chondrocytes (HC)

text: primary culture, chondrocytes

text: Homo sapiens


text: Helix Research Institute

text: live

text: Generic

text: CD34+ cells after 3-days ODF induction

text: primary culture, CD34+ cells

text: Homo sapiens

text: cord blood


text: Helix Research Institute

text: live

text: Generic

text: fetal

text: Homo sapiens

text: brain


text: Helix Research Institute

text: live

text: Generic

text: CD34+ cells after 6-days ODF induction

text: primary culture, CD34+ cells

text: Homo sapiens

text: cord blood


text: Helix Research Institute

text: live

text: Generic

text: CD34+ cells after 9-days ODF induction

text: primary culture, CD34+ cells

text: Homo sapiens

text: cord blood


text: Helix Research Institute

text: live

text: Generic

text: fetal

text: Homo sapiens

text: liver


text: Helix Research Institute

text: live

text: Generic

text: fetal

text: Homo sapiens

text: lung


text: Helix Research Institute

text: live

text: Generic

text: neutrophils

text: primary culture, neutrophils

text: Homo sapiens


text: Helix Research Institute

text: live

text: Generic

text: neuroblastoma

text: Homo sapiens


text: Helix Research Institute

text: live

text: Generic

text: erythroleukemia

text: Homo sapiens


text: Helix Research Institute

text: live

text: Generic

text: leukemia cell line (myelogenous)

text: Homo sapiens


text: Helix Research Institute

text: live

text: Generic

text: Homo sapiens

text: thalamus


text: Helix Research Institute

text: live

text: Generic

text: Homo sapiens

text: heart


text: Helix Research Institute

text: live

text: Generic

text: synoviocytes from rheumatioid arthritis (HS-RA)

text: primary culture, synoviocytes from rheumatioid arthritis

text: Homo sapiens


text: The University of Tokyo

text: live

text: Generic

text: polyps were inhabited by Symbiodinium spp. strain PL-TS-1 (clade A)

text: juveniles approximately 3 weeks after metamorphosis

text: Acropora tenuis

text: whole polyps

text: Acropora tenuis juvenile whole polyps


text: Helix Research Institute

text: live

text: Generic

text: Homo sapiens

text: liver


text: Helix Research Institute

text: live

text: Generic

text: Homo sapiens

text: esophagus


text: Helix Research Institute

text: live

text: Generic

text: adult

text: Homo sapiens

text: ovary


text: Helix Research Institute

text: live

text: Generic

text: Homo sapiens

text: cervix


text: Helix Research Institute

text: live

text: Generic

text: lymphoblasts (EB virus transferred B cell)

text: primary culture, lymphoblasts

text: Homo sapiens


text: Helix Research Institute

text: live

text: Generic

text: teratocarcinoma

text: Homo sapiens


text: Helix Research Institute

text: live

text: Generic

text: dermal papilla cells (HDPC)

text: primary culture, dermal papilla cells

text: Homo sapiens


text: Helix Research Institute

text: live

text: Generic

text: Homo sapiens

text: mammary gland


text: Helix Research Institute

text: live

text: Generic

text: CD34+ cells after 3-days ODF induction

text: primary culture, CD34+ cells

text: Homo sapiens

text: cord blood


text: Helix Research Institute

text: live

text: Generic

text: fetal

text: Homo sapiens

text: heart


text: Helix Research Institute

text: live

text: Generic

text: HeLa cells

text: HeLa

text: Homo sapiens


text: The Canadian Potato Genome Project - BioAtlantech

text: live

text: Generic

text: Shepody

characteristic: cultivar

text: supplier: Developmental series. Plants from pathogen-free Solanum tuberosum var. Shepody, clone 1756, nuclear stock were grown in a screenhouse under natural conditions. Mature, harvested tubers were stored in the dark at 4C, 95% relative humidity for 3 months, and then reconditioned at 21C for three days. RNA was isolated for library construction.

characteristic: lab-host

text: Solanum tuberosum

text: EcoRI

text: XhoI

text: Tubers

text: Cold Sweetening C

text: pBluescript II SK(+) XR


text: National Taiwan University

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Solid-cultured basidiom of A. cinnamomea obtained from Division of Forest Protectin of Taiwan Forestry Research Institute. The cDNA library was constructed by pGEM-T Easy vector system (Promega).

text: Solid-cultured

text: Taiwanofungus camphoratus

text: basidiom

text: pGEM-T Easy


text: National Taiwan University

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Solid-cultured mycelia of A. cinnamomea obtained from Division of Forest Protectin of Taiwan Forestry Research Institute. The cDNA library was constructed by pGEM-T Easy vector system (Promega).

text: Solid-cultured

text: Taiwanofungus camphoratus

text: mycelia

text: Solid-cultured mycelia of A. cinnamomea

text: pGEM-T Easy


text: National Taiwan University

text: live

text: Generic

text: Liquid-cultured mycelia of A. cinnamomea obtained from Division of Forest Protectin of Taiwan Forestry Research Institute. The cDNA library was constructed by pGEM-T Easy vector system (Promega).

text: Liquid-cultured

characteristic: dev-stage

text: Taiwanofungus camphoratus

text: mycelia

text: pGEM-T Easy


text: National Taiwan University

text: live

text: Generic

text: Wild-type basidiom of A. cinnamomea obtained from Division of Forest Protectin of Taiwan Forestry Research Institute. The cDNA library was constructed by pGEM-T Easy vector system (Promega).

text: Wild-type

text: Taiwanofungus camphoratus

characteristic: organism

text: basidiom

text: Wild-type basidiom of A. cinnamomea

text: pGEM-T Easy


text: Cardiff University

text: live

text: Generic

text: Gammarus pulex female subtractive library

text: Adult

text: Gammarus pulex

text: Female

characteristic: sex

text: feS

text: pGEM-T


text: Agricultural research organization, Newe Yaiar research center

text: live

text: Generic

text: Noy Yizre'el

text: Library constructed using Clontech PCR-Select cDNA Subtraction kit. Tester: mixed stage fruits from cultivar Noy Yizre'el; Driver: mixed stage fruits from cultivar Vedrantais. More information is available at http://melon.bti.cornell.edu

text: mixed stages

text: Cucumis melo

text: 5' EcoRI

text: 3' XhoI

text: fruit

text: Melon subtraction library 1

text: Plasmid

text: pGEM-T easy


text: live

text: Generic

text: Vedrantais

text: Library constructed using Clontech PCR-Select cDNA Subtraction kit. Tester: mixed stage fruits from cultivar Vedrantais; Driver: mixed stage fruits from cultivar Noy Yizre'el. More information is available at http://melon.bti.cornell.edu

text: mixed stages

text: Cucumis melo

text: 5' EcoRI

text: 3' XhoI

text: fruit

text: Melon subtraction library 2

text: Plasmid

text: pGEM-T easy


text: live

text: Generic

text: Library constructed using Clontech PCR-Select cDNA Subtraction kit. Tester: roots, stems and leaves; Driver: mixed stage fruits. More information is available at http://melon.bti.cornell.edu

text: Cucumis melo

text: 5' EcoRI

text: 3' XhoI

text: roots, stems and leaves

text: Melon subtraction library 3

text: Plasmid

text: pGEM-T easy


text: live

text: Generic

text: Library constructed using Clontech PCR-Select cDNA Subtraction kit. Tester: mixed stage fruits; Driver: roots, stems and leaves; More information is available at http://melon.bti.cornell.edu

text: mixed stages

text: Cucumis melo

text: 5' EcoRI

text: 3' XhoI

text: fruit

text: Melon subtraction library 4

text: Plasmid

text: pGEM-T easy


text: live

text: Generic

text: Dulce

text: Library constructed using Clontech PCR-Select cDNA Subtraction kit. Tester: mixed stage fruits from cultivar Dulce; Driver: mixed stage fruits from cultivar PI414723. More information is available at http://melon.bti.cornell.edu

text: mixed stages

text: Cucumis melo

text: 5' EcoRI

text: 3' XhoI

text: fruit

text: Melon subtraction library 5

text: Plasmid

text: pGEM-T easy


text: live

text: Generic

text: Library constructed using Clontech PCR-Select cDNA Subtraction kit. Tester: mixed stage fruits from cultivar PI414723; Driver: mixed stage fruits from cultivar Dulce. More information is available at http://melon.bti.cornell.edu

text: mixed stages

text: Cucumis melo

text: 5' EcoRI

text: 3' XhoI

text: fruit

text: Melon subtraction library 6

text: Plasmid

text: pGEM-T easy


text: live

text: Generic

text: Roshet

text: Library constructed using Clontech PCR-Select cDNA Subtraction kit. Tester: mixed stage fruits from cultivar Rochet; Driver: mixed stage fruits from cultivar Noy Yizre'el. More information is available at http://melon.bti.cornell.edu

text: mixed stages

text: Cucumis melo

text: 5' EcoRI

text: 3' XhoI

text: fruit

text: Melon subtraction library 7

text: Plasmid

text: pGEM-T easy


text: live

text: Generic

text: Noy Yizre'el

text: Library constructed using Clontech PCR-Select cDNA Subtraction kit. Tester: mixed stage fruits from cultivar Noy Yizre'el; Driver: mixed stage fruits from cultivar Rochet. More information is available at http://melon.bti.cornell.edu

text: mixed stages

text: Cucumis melo

text: 5' EcoRI

text: 3' XhoI

text: fruit

text: Melon subtraction library 8

text: Plasmid

text: pGEM-T easy


text: live

text: Generic

text: Dulce

text: Library constructed using Clontech PCR-Select cDNA Subtraction kit. Tester: mixed stage fruits from cultivar Dulce; Driver: mixed stage fruits from cultivar Noy Yizre'el. More information is available at http://melon.bti.cornell.edu

text: mixed stages

text: Cucumis melo

text: 5' EcoRI

text: 3' XhoI

text: fruit

text: Melon subtraction library 9

text: Plasmid

text: pGEM-T easy


text: live

text: Generic

text: Faqus

text: Library constructed using Clontech PCR-Select cDNA Subtraction kit. Tester: mixed stage fruits from cultivar Faqqous; Driver: mixed stage fruits from cultivar Noy Yizre'el. More information is available at http://melon.bti.cornell.edu

text: mixed stages

text: Cucumis melo

text: 5' EcoRI

text: 3' XhoI

text: fruit

text: Melon subtraction library 10

text: Plasmid

text: pGEM-T easy


text: live

text: Generic

text: Noy Yizre'el

text: Library constructed using Clontech PCR-Select cDNA Subtraction kit. Tester: mixed stage fruits from cultivar Noy Yizre'el; Driver: mixed stage fruits from cultivar Faqqous. More information is available at http://melon.bti.cornell.edu

text: mixed stages

text: Cucumis melo

text: 5' EcoRI

text: 3' XhoI

text: fruit

text: Melon subtraction library 11

text: Plasmid

text: pGEM-T easy


text: live

text: Generic

text: Noy Yizre'el

text: Library constructed using Clontech PCR-Select cDNA Subtraction kit. Tester: Mature flesh (47DPA); Driver: Young flesh (47DPA). More information is available at http://melon.bti.cornell.edu

text: 47 DPA

text: Cucumis melo

text: 5' EcoRI

text: 3' XhoI

text: mature flesh

text: Melon subtraction library 12

text: Plasmid

text: pGEM-T easy


text: live

text: Generic

text: Noy Yizre'el

text: Library constructed using Clontech PCR-Select cDNA Subtraction kit. Tester: Young flesh (47DPA); Driver: Mature flesh (47DPA). More information is available at http://melon.bti.cornell.edu

text: 25 DPA

text: Cucumis melo

text: 5' EcoRI

text: 3' XhoI

text: young flesh

text: Melon subtraction library 13

text: Plasmid

text: pGEM-T easy


text: live

text: Generic

text: Noy Yizre'el

text: Library constructed using Clontech PCR-Select cDNA Subtraction kit. Tester: Mature rind (47DPA); Driver: Young rind (47DPA). More information is available at http://melon.bti.cornell.edu

text: 47 DPA

text: Cucumis melo

text: 5' EcoRI

text: 3' XhoI

text: mature rind

text: Melon subtraction library 14

text: Plasmid

text: pGEM-T easy


text: live

text: Generic

text: Noy Yizre'el

text: Library constructed using Clontech PCR-Select cDNA Subtraction kit. Tester: Young rind (47DPA); Driver: Mature rind (47DPA). More information is available at http://melon.bti.cornell.edu

text: 25 DPA

text: Cucumis melo

text: 5' EcoRI

text: 3' XhoI

text: young rind

text: Melon subtraction library 15

text: Plasmid

text: pGEM-T easy


text: live

text: Generic

text: F2 Noy Yizre'el and Faqqous

text: Library constructed using Clontech PCR-Select cDNA Subtraction kit. Tester: High sucrose; Driver: Low sucrose. More information is available at http://melon.bti.cornell.edu

text: mature

text: Cucumis melo

text: 5' EcoRI

text: 3' XhoI

text: fruit

text: Melon subtraction library 16

text: Plasmid

text: pGEM-T easy


text: Agricultural research organization, Newe Yaiar research center

text: live

text: Generic

text: F2 Noy Yizre'el and Faqqous

text: Library constructed using Clontech PCR-Select cDNA Subtraction kit. Tester: Low sucrose; Driver: High sucrose. More information is available at http://melon.bti.cornell.edu

text: mature

text: Cucumis melo

text: 5' EcoRI

text: 3' XhoI

text: fruit

text: Melon subtraction library 17

text: Plasmid

text: pGEM-T easy


text: Agricultural Research Organization

text: live

text: Generic

text: Noy Yizre'el

text: Partial sequences, cloned based on homology to conserved sequences. More information is available at http://melon.bti.cornell.edu

text: Cucumis melo

text: 5' EcoRI

text: 3' XhoI

text: fruit

text: Melon carotenoid or organic acid metabolism related gene library

text: Plasmid

text: pGEM-T easy


text: Agricultural Research Organization

text: live

text: Generic

text: Noy Yizre'el

text: Partial sequences, cloned based on homology to conserved sequences More information is available at http://melon.bti.cornell.edu

text: Cucumis melo

text: 5' EcoRI

text: 3' XhoI

text: fruit

text: Melon sugar metabolism related gene library

text: Plasmid

text: pGEM-T easy


text: live

text: Generic

text: Noy Yizre'el

text: Library constructed using Stratagene ZAP cDNA Synthesis kit. More information is available at http://melon.bti.cornell.edu

text: a mixture of 35 DPA and 37 DPA

text: Cucumis melo

text: 5' EcoRI

text: 3' XhoI

text: a mixture of mature green and mature yellow fruits

text: Melon mature fruit library

text: Plasmid

text: pBK-CMV


text: live

text: Generic

text: Noy Yizre'el

text: a mixture of fruits at 0, 1, 3, 12, and 25 DPA

text: Cucumis melo

text: 5' EcoRI

text: 3' XhoI

text: fruit

text: Melon young fruit library

text: Plasmid

text: pBK-CMV


text: live

text: Generic

text: Dulce

text: mature

text: Cucumis melo

text: 5' EcoRI

text: 3' XhoI

text: fruit

text: Dulce mature fruit library

text: Plasmid

text: pBK-CMV


text: live

text: Generic

text: Tam Dew

text: mature

text: Cucumis melo

text: 5' EcoRI

text: 3' XhoI

text: fruit

text: Tam Dew mature fruit library

text: Plasmid

text: pBK-CMV


text: live

text: Generic

text: More information is available at http://melon.bti.cornell.edu

text: Cucumis melo

text: 5' EcoRI

text: 3' XhoI

text: Melon mixed library

text: Plasmid

text: pGEM-T easy


text: Helix Research Institute

text: live

text: Generic

text: fetal

text: Homo sapiens

text: brain


text: Helix Research Institute

text: live

text: Generic

text: Homo sapiens

text: placenta


text: Helix Research Institute

text: live

text: Generic

text: Homo sapiens

text: prostate


text: Helix Research Institute

text: live

text: Generic

text: pulmonary artery endothelial cells (HPAEC)

characteristic: cell type

text: primary culture, pulmonary artery endothelial cells

text: Homo sapiens


text: Helix Research Institute

text: live

text: Generic

text: neuroblastoma

text: SK-N-SH

characteristic: cell-line

text: Homo sapiens

characteristic: title


characteristic: External Id

text: Helix Research Institute

text: live

text: Generic

text: Homo sapiens

text: small intestine

characteristic: tissue-type


text: Helix Research Institute

text: live

text: Generic

text: Homo sapiens

text: spleen

characteristic: vector


text: Helix Research Institute

characteristic: INSDC center name

text: live

text: Generic

text: Homo sapiens

text: breast, tumor tissue


text: Helix Research Institute

characteristic: INSDC first public

text: live

text: Generic

text: Homo sapiens

text: testis


text: Helix Research Institute

text: live

text: Generic

text: teratocarcinoma

text: majorly NT2 neuron; mRNA from NT2 neuronal precursor cells treated 2-weeks mitotic inhibitor after 5-weeks retinoic acid (RA) induction

characteristic: description

text: Homo sapiens


text: Helix Research Institute

text: live

text: Generic

text: teratocarcinoma

text: mRNA from NT2 neuronal precursor cells after 5-weeks retinoic acid (RA) induction

text: Homo sapiens


text: Helix Research Institute

text: live

text: Generic

text: Homo sapiens

text: tongue


text: Helix Research Institute

text: live

text: Generic

text: Homo sapiens

text: synovial membrane tissue from rheumatioid arthritis


text: Helix Research Institute

text: live

text: Generic

text: Homo sapiens

text: rectum


text: Helix Research Institute

text: live

text: Generic

text: Homo sapiens

text: stomach


text: Helix Research Institute

text: live

text: Generic

text: Homo sapiens

text: colon, tumor tissue


text: Helix Research Institute

text: live

text: Generic

text: Homo sapiens

text: pancreas


text: Helix Research Institute

text: live

text: Generic

text: peripheral blood mononuclear cells (HPBMC5939)

text: primary culture, peripheral blood mononuclear cells

text: Homo sapiens


text: Helix Research Institute

text: live

text: Generic

text: Homo sapiens

text: esophageal, tumor tissue


text: Helix Research Institute

text: live

text: Generic

text: Homo sapiens

text: salivary gland


text: Helix Research Institute

text: live

text: Generic

text: Homo sapiens

text: skeletal muscle


text: Helix Research Institute

text: live

text: Generic

text: Homo sapiens

text: pericardium


text: Helix Research Institute

text: live

text: Generic

text: Homo sapiens

text: cervix, tumor tissue


text: Helix Research Institute

text: live

text: Generic

text: neuroepithelioma

text: SK-N-MC

text: Homo sapiens


text: Helix Research Institute

text: live

text: Generic

text: Homo sapiens

text: prostate


text: Helix Research Institute

text: live

text: Generic

text: teratocarcinoma

text: mRNA from NT2 neuronal precursor cells treated 2-weeks mitotic inhibitor after 5-weeks retinoic acid (RA) induction

text: Homo sapiens


text: Helix Research Institute

text: live

text: Generic

text: Homo sapiens

text: placenta


text: Helix Research Institute

text: live

text: Generic

text: Homo sapiens


text: Helix Research Institute

text: live

text: Generic

text: normal astrocytes (NHA5732)

text: primary culture, normal astrocytes

text: Homo sapiens


text: Helix Research Institute

text: live

text: Generic

text: Homo sapiens

text: testis


text: Helix Research Institute

text: live

text: Generic

text: Homo sapiens

text: thymus


text: Helix Research Institute

text: live

text: Generic

text: Homo sapiens

text: lung, tumor tissue


text: Helix Research Institute

text: live

text: Generic

text: Homo sapiens

text: trachea


text: Helix Research Institute

text: live

text: Generic

text: Homo sapiens

text: uterus


text: Helix Research Institute

text: live

text: Generic

text: Homo sapiens

text: kidney, tumor tissue


text: Helix Research Institute

text: live

text: Generic

text: Homo sapiens

text: liver, tumor tissue


text: Helix Research Institute

text: live

text: Generic

text: Homo sapiens

text: ovary, tumor tissue


text: Helix Research Institute

text: live

text: Generic

text: Homo sapiens

text: stomach, tumor tissue


text: Helix Research Institute

text: live

text: Generic

text: Homo sapiens

text: uterus, tumor tissue


text: Helix Research Institute

text: live

text: Generic

text: umbilical vein endothelial cells (HUVEC)

text: primary culture, umbilical vein endothelial cells

text: Homo sapiens


text: Helix Research Institute

text: live

text: Generic

text: primary culture, endothelial cells

text: Homo sapiens


text: European Molecular Biology Laboratory (EMBL)

text: live

text: Generic

text: This cDNA library was generated by Kristin Tessmar-Raible and Heidi Snyman. cDNA was prepared from poly-A RNA extracted from four batches of Platynereis dumerilii larvae at 48hpf. A poly-T NotI primer adapter was used for the first reverse transcriptase reaction. SalI adapters were added, then fragments were NotI digested and directionally cloned into the SalI/NotI restriction site of the pCMVSport6 vector (Invitrogen).

text: 48 hours post fertilization

text: Platynereis dumerilii

text: SalI

text: NotI

text: Platynereis dumerilii 48hpf library

text: Plasmid


text: The University of Melbourne

text: live

text: Generic

text: Sclerotinia sclerotiorum (strain UQ1280) was cultured on water agar containing chopped, autoclaved Brassica napus Surpass400 leaves.Mycelium was collected and used to make the library. Single pass sequencing was done using SMART sequencing primer (TATCAACGCAGAGTACGCGG)

text: E. coli

characteristic: lab-host

text: Sclerotinia sclerotiorum

characteristic: strain

text: mycelia

text: Sclerotinia sclerotiorum mycelia EST library

text: Plasmid

text: pGem-T Easy


text: The University of Melbourne

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Sclerotinia sclerotiorum (strain UQ1280) was inoculated onto Brassica napus Surpass400 leaves. A section of lesion and adjacent tissue was collected and used to make the library. Fungal sequences were identified using BLASTX comparison with known plant and fungal sequences. Single pass sequencing was done using SMART sequencing primer (TATCAACGCAGAGTACGCGG)

text: E. coli

text: Sclerotinia sclerotiorum

text: infected leaf

text: Sclerotinia sclerotiorum from Brassica napus leaf infected with Sclerotinia sclerotiorum

text: Plasmid

text: pGem-T Easy


text: The University of Melbourne

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Surpass 400

characteristic: cultivar

text: Sclerotinia sclerotiorum (strain UQ1280) was inoculated onto Brassica napus Surpass400 leaves. A section of lesion and adjacent tissue was collected and used to make the library. Plant sequences were identified using BLASTX comparison with known plant and fungal sequences. Single pass sequencing was done using SMART sequencing primer (TATCAACGCAGAGTACGCGG)

text: E. coli

text: Brassica napus

text: infected leaf

text: Brassica napus leaf infected with Sclerotinia sclerotiorum EST library

text: Plasmid

text: pGem-T Easy


text: The University of Melbourne

text: live

text: Generic

text: Agar plugs from the growing edge of a Sclerotinia sclerotiorum (strain UQ1280) colony were transferred to empty sterile Petri dishes for 24 h. Infection cushions(complex appressoria) were collected and used to make the library. Single pass sequencing was done using SMART sequencing primer (TATCAACGCAGAGTACGCGG)

text: E. coli

text: Sclerotinia sclerotiorum

characteristic: organism

text: infection cushion(appressoria)

text: Sclerotinia sclerotiorum infection cushion (appressoria) EST library

text: Plasmid

text: pGem-T Easy


text: University of Agricultural Sciences

text: live

text: Generic

text: Bunch Type

text: late vegetative

characteristic: dev-stage

text: Arachis hypogaea

text: leaf

text: DCPSM 87


text: University of Agricultural Sciences

text: live

text: Generic

text: early vegetative

text: Nicotiana benthamiana

text: moisture-stressed leaves

text: DCPSM NBH1


text: University of Agricultural Sciences

text: live

text: Generic

text: late vegetative

text: Arachis hypogaea

text: moisture-stressed leaf

text: DCPSM PH15


text: Helix Research Institute

text: live

text: Generic

text: normal neural progenitor cells (NHNP5958)

characteristic: cell type

text: primary culture, normal neural progenitor cells

text: Homo sapiens


text: University of St Andrews

text: live

text: Generic

text: Salmo salar

text: Fast muscle

text: EST library from fast muscle of Atlantic salmon, Salmo salar

characteristic: title


characteristic: External Id

text: Cornell University

text: live

text: Generic

text: 18-22 weeks after pollination, early cherry development stage

text: Coffea canephora

text: ECoRI

text: XhoI

text: whole cherry

text: Cherry of Early Development Stage

text: Plasmid

text: pBSSK-


text: Cornell University

text: live

text: Generic

text: young

text: Coffea canephora

text: ECoRI

text: XhoI

text: leaf

characteristic: tissue-type

text: Leaf

text: Plasmid

text: pBSSK-


text: Cornell University

text: live

text: Generic

text: 42-46 weeks after pollination, late seed development stage

text: Coffea canephora

text: ECoRI

text: XhoI

text: endorsperm and perisperm

text: Seed of Late Development Stage

text: Plasmid

text: pBSSK-

characteristic: vector