text: National Institute of Genetics

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: synonym: Physcomitrella patens subsp. patens

text: young sporophytes cultivated for 3-4 weeks at 15C under short-day conditions

text: Physcomitrium patens

text: sporophytes (embryos) with surrounding archegonia

characteristic: tissue-type

text: ppsp full-length cDNA libary


status: PUBLIC

characteristic: External Id

text: The University of Georgia, Department of Plant Biology

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: The library was prepared from polyA+ RNA from the roots of 1-year-old loblolly pine (Pinus taeda) cuttings that were rooted and then planted in washed sand. The rooted cuttings were maintained for 139 days (July 28 2003 harvest) under ambient conditions in a local greenhouse. They were kept on a weekly regimen of 0.5x nutrient-complete Hoagland's solution and supplemented with additional water sufficient to maintain a 15% soil moisture content. Starting five weeks prior to harvesting roots for mRNA preparation, the weekly fertilizer regimen for the potted trees was modified to omit magnesium from the 0.5x Hoagland's solution (5 applications). For two days prior to starting the modified fertilizer regimen, pots were flushed extensively with water to reduce residual levels of magnesium. Double-stranded cDNA was cloned unidirectionally into pSL1180. Inserts can be excised with EcoRI (5' end) and XhoI (3' end).

characteristic: description

text: DH10B-T1 phage-resistant E. coli

tag: attribute

characteristic: lab-host

text: Root

characteristic: organ

text: Pinus taeda

characteristic: organism

text: EcoRI

text: XhoI

text: 3 CCLONES

characteristic: strain

text: Roots minus magnesium

characteristic: title

characteristic: vector


text: Justus-Liebig-University

text: live

text: Generic

text: Ingrid

characteristic: cultivar

text: 7-day-old seedling

characteristic: dev-stage

text: Hordeum vulgare subsp. vulgare

text: leaf

text: barley leaf SSH


text: National Institute of Genetics

characteristic: INSDC last update

text: live

text: Generic

text: embryo just before hatching

text: Molgula tectiformis

text: whole animal

text: Molgula tectiformis unpublished cDNA library


text: National Institute of Genetics

text: live

characteristic: INSDC status

text: Generic

text: cleaving embryo

text: Molgula tectiformis

text: whole animal

text: Molgula tectiformis unpublished cDNA library, cleaving embryo


text: National Institute of Genetics

text: live

text: Generic

text: mixture of gastrula and neurula

text: Molgula tectiformis

text: whole animal

characteristic: tissue-type

text: Molgula tectiformis unpublished cDNA library, mixture of gastrula and neurula


characteristic: External Id

text: National Institute of Genetics

text: live

text: Generic

text: Molgula tectiformis

text: gonad

text: Molgula tectiformis unpublished cDNA library, gonad


text: The Royal Veterinary and Agricultural University

characteristic: INSDC center name

text: live

text: Generic

text: Sus scrofa

text: Pig pBluescript and pTrueBlue library

text: Plasmid


text: National Institute of Genetics

characteristic: INSDC first public

text: live

text: Generic

text: larva

text: Molgula tectiformis

text: whole animal

text: Molgula tectiformis unpublished cDNA library, larva


text: National University of Ireland Maynooth

text: live

text: Generic

characteristic: NCBI submission model

text: Nematodes were desiccated at 85% RH for 24 hours before RNA was extracted.

text: Dauer

text: Steinernema carpocapsae

text: All

text: Steinernema carpocapsae desiccation expressed library


text: Baylor College of Medicine

text: live

text: Generic

characteristic: NCBI submission package

text: oligo dt priming from poly A+ RNA, directionally cloned

text: 0-18h embryos

text: Drosophila pseudoobscura

text: EcoRI

text: XhoI

text: Drosophila pseudoobscura embryonic cDNA library

text: plasmid


text: live

text: Generic

text: The library was prepared from polyA+ RNA from the roots of 1-year-old loblolly pine (Pinus taeda) cuttings that were rooted and then planted in washed sand. The rooted cuttings were maintained for 139 days (July 28 2003 harvest) under ambient conditions in a local greenhouse. They were kept on a weekly regimen of 0.5x nutrient-complete Hoagland's solution and supplemented with additional water sufficient to maintain a 15% soil moisture content. Starting five weeks prior to harvesting roots for mRNA preparation, the weekly fertilizer regimen for the potted trees was modified to omit iron from the 0.5x Hoagland's solution (5 applications). For two days prior to starting the modified fertilizer regimen, pots were flushed extensively with water to reduce residual levels of iron. Double-stranded cDNA was cloned unidirectionally into pSL1180. Inserts can be excised with EcoRI (5' end) and XhoI (3' end).

characteristic: description

text: DH10B-T1 phage-resistant E. coli

text: Root

text: Pinus taeda

text: EcoRI

text: XhoI

text: 3 CCLONES

text: Roots minus iron


text: Universidade de Sao Paulo - USP - FCFRP

text: live

text: Generic

text: YEAST

text: Paracoccidioides brasiliensis

text: YEAST

text: Plamid

text: pCMVSPORT 6.0


text: live

text: Generic

text: The library was prepared from polyA+ RNA from the roots of 1-year-old loblolly pine (Pinus taeda) cuttings that were rooted and then planted in washed sand. The rooted cuttings were maintained for 139 days (July 28 2003 harvest) under ambient conditions in a local greenhouse. They were kept on a weekly regimen of 0.5x nutrient-complete Hoagland's solution and supplemented with additional water sufficient to maintain a 15% soil moisture content. Starting five weeks prior to harvesting roots for mRNA preparation, the weekly fertilizer regimen for the potted trees was modified to omit phosphorus from the 0.5x Hoagland's solution (5 applications). For two days prior to starting the modified fertilizer regimen, pots were flushed extensively with water to reduce residual levels of phosphorus. Double-stranded cDNA was cloned unidirectionally into pSL1180. Inserts can be excised with EcoRI (5' end) and XhoI (3' end).

text: DH10B-T1 phage-resistant E. coli

characteristic: lab-host

text: Root

text: Pinus taeda

text: EcoRI

text: XhoI

text: 3 CCLONES

text: Roots minus phosphorous


text: University of California at Davis

text: live

text: Generic

text: Forward subtracted SSH library of Triphysaria versicolor (parasitic plant) root tips in contact with Arabidopsis (host) roots for 0 to 5 hrs.

text: early, pre-attachment haustorium development

text: root

characteristic: organ

text: Triphysaria versicolor

text: Triphysaria versicolor (parasitic plant) root-tip, host forward SSH library


text: University of California at Davis

text: live

text: Generic

text: Reverse subtracted SSH library of Triphysaria versicolor (parasitic plant) root tips in contact with Arabidopsis (host) roots for 0 to 5 hrs.

text: root

text: Triphysaria versicolor

characteristic: organism

text: Triphysaria versicolor (parasitic plant) root-tip, host reverse SSH library


text: Huazhong Agricultural University

text: live

text: Generic

text: 10 and 20 days post anthesis (dpa)

text: Gossypium hirsutum

characteristic: strain

text: fiber

text: cotton fiber suppression subtractive hybridization Library


text: Nanjing Forestry University

text: live

text: Generic

text: 2 year old tree

text: Populus deltoides

text: young leaf

text: Populus deltoides young leaf

characteristic: title


text: The University of Georgia, Department of Plant Biology

text: live

text: Generic

text: The library was prepared from polyA+ RNA from the roots of 1-year-old loblolly pine (Pinus taeda) cuttings that were rooted and then planted in washed sand. The rooted cuttings were maintained for 139 days (July 28 2003 harvest) under ambient conditions in a local greenhouse. They were kept on a weekly regimen of 0.5x nutrient-complete Hoagland's solution and supplemented with additional water sufficient to maintain a 15% soil moisture content. Starting five weeks prior to harvesting roots for mRNA preparation, the weekly fertilizer regimen for the potted trees was modified to omit micronutrients (boron, copper, manganese, molybdenum, zinc) from the 0.5x Hoagland's solution (5 applications). For two days prior to starting the modified fertilizer regimen, pots were flushed extensively with water to reduce residual levels of micronutrients. Double-stranded cDNA was cloned unidirectionally into pSL1180. Inserts can be excised with EcoRI (5' end) and XhoI (3' end).

text: DH10B-T1 phage-resistant E. coli

text: Root

text: Pinus taeda

text: EcoRI

text: XhoI

text: 3 CCLONES

text: Roots minus micronutrients

characteristic: vector


text: Ceres, Inc

text: live

text: Generic

text: Arabidopsis thaliana

text: Mixed, WS and Landsberg erecta

text: Mixed floral buds, roots (9:1)


text: Ceres, Inc

text: live

text: Generic

text: Arabidopsis thaliana

text: WS

text: Flower, leaf and root


text: Ceres, Inc

text: live

text: Generic

text: Arabidopsis thaliana

text: WS

text: Flower, leaf and root

text: CERES-AN


text: JT Biohistory Research Hall

text: live

text: Generic

text: Papilio xuthus

text: Papilio xuthus library (Yoshikawa H)


text: Wellcome Trust Sanger Institute

text: live

text: Generic

text: Homo sapiens

text: foetal lung

text: Homo sapiens foetal lung


text: Wellcome Trust Sanger Institute

text: live

text: Generic

text: Homo sapiens

text: foetal stomach

text: Homo sapiens foetal stomach


text: Wellcome Trust Sanger Institute

text: live

text: Generic

text: Homo sapiens

text: foetal brain

text: Homo sapiens foetal brain


text: Wellcome Trust Sanger Institute

text: live

text: Generic

text: Homo sapiens

text: bladder

text: Homo sapiens bladder


text: Wellcome Trust Sanger Institute

text: live

text: Generic

text: Homo sapiens

text: foetal skeletal muscle

text: Homo sapiens foetal skeletal muscle


text: Wellcome Trust Sanger Institute

text: live

text: Generic

text: Homo sapiens

text: brain

text: Homo sapiens brain


text: Wellcome Trust Sanger Institute

text: live

text: Generic

text: Homo sapiens

text: foetal kidney

text: Homo sapiens foetal kidney


text: Wellcome Trust Sanger Institute

text: live

text: Generic

text: Homo sapiens

text: heart

text: Homo sapiens heart


text: Wellcome Trust Sanger Institute

text: live

text: Generic

text: Homo sapiens

text: foetal colon

text: Homo sapiens foetal colon


text: Wellcome Trust Sanger Institute

text: live

text: Generic

text: Homo sapiens

text: lung

text: Homo sapiens lung


text: Wellcome Trust Sanger Institute

text: live

text: Generic

text: Homo sapiens

text: foetal heart

text: Homo sapiens foetal heart


text: Wellcome Trust Sanger Institute

text: live

text: Generic

text: Homo sapiens

text: cervix

text: Homo sapiens cervix


text: Wellcome Trust Sanger Institute

text: live

text: Generic

text: Homo sapiens

text: adrenal gland

text: Homo sapiens adrenal gland


text: Wellcome Trust Sanger Institute

text: live

text: Generic

text: Homo sapiens

text: ovary

text: Homo sapiens ovary


text: Wellcome Trust Sanger Institute

text: live

text: Generic

text: Homo sapiens

text: colon

text: Homo sapiens colon


text: Wellcome Trust Sanger Institute

text: live

text: Generic

text: Homo sapiens

text: uterus

text: Homo sapiens uterus


text: Wellcome Trust Sanger Institute

text: live

text: Generic

text: Homo sapiens

text: prostate

text: Homo sapiens prostate


text: Wellcome Trust Sanger Institute

text: live

text: Generic

text: Homo sapiens

text: small intestine

text: Homo sapiens small intestine (Eades)


text: Wellcome Trust Sanger Institute

text: live

text: Generic

text: Homo sapiens

text: bone marrow

text: Homo sapiens bone marrow (Eades)


text: Wellcome Trust Sanger Institute

text: live

text: Generic

text: Homo sapiens

text: liver

text: Homo sapiens liver (Eades)


text: Wellcome Trust Sanger Institute

text: live

text: Generic

text: Homo sapiens

text: testis

text: Homo sapiens testis (Eades)


text: Wellcome Trust Sanger Institute

text: live

text: Generic

text: Homo sapiens

text: stomach

text: Homo sapiens stomach (Eades)


text: Roslin Institute

text: live

text: Generic

text: bovine monocyte

characteristic: cell type

text: clone library constructed from pooled Bos taurus and Bos indicus monocytes

text: Hyalomma anatolicum

text: blood

text: KN206, Hyalomma anatolicum


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: vector: http://www.rzpd.de/info/vectors/pQE80LSN_cloned_pic.shtml ; 1st strand cDNA was prepared from mRNA obtained from human T-Lymphocytes with a NotI - oligo(dT) primer [5' GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT 3']. Double-stranded cDNA was ligated to SalI adaptors, digested with NotI and cloned into the NotI and SalI sites of the pQE80LSN_cloned vector

text: adult

characteristic: dev-stage

text: Homo sapiens

text: SalI

text: NotI

text: T-Lymphocytes

text: RZPD no.9016


characteristic: INSDC last update

text: live

text: Generic

text: adult

text: Homo sapiens

text: SalI

text: NotI

text: T-Lymphocytes

text: RZPD no.9017


text: live

characteristic: INSDC status

text: Generic

text: The library was prepared from polyA+ RNA from the roots of 1-year-old loblolly pine (Pinus taeda) cuttings that were rooted and then planted in washed sand. The rooted cuttings were maintained for 154 days (August 12 2003 harvest) under ambient conditions in a local greenhouse. They were kept on a weekly regimen of 0.5x nutrient-complete Hoagland's solution and supplemented with additional water sufficient to maintain a 15% soil moisture content. Twenty-four hours (24h) prior to harvesting roots for mRNA preparation, the potted trees were watered with 500 uM HgCl2 (aq) until the sand was saturated. Double-stranded cDNA was cloned unidirectionally into pSL1180. Inserts can be excised with EcoRI (5' end) and XhoI (3' end).

text: DH10B-T1 phage-resistant E. coli

text: Root

text: Pinus taeda

text: EcoRI

text: XhoI

text: 3 CCLONES

text: Roots plus added mercury


text: USDA - ARS

text: live

text: Generic

text: A high quality EST with at least 150 contiguous bases at Trace Tuner score of 20 or better. cDNA Library construction by Lawrence Lee.

text: Adult Alate

text: Aphis gossypii

text: EcoRI

text: XhoI

text: Mixed population

characteristic: sex

text: Entire insect

characteristic: tissue-type

text: Adult Alate Aphis gossypii (WHAGA)

text: pBluescript II SK+


characteristic: External Id

text: University of Nevada, Reno

characteristic: INSDC center name

text: live

text: Generic

text: Total RNA was used for ds cDNA synthesis using Clontech SMART protocol (Zhu et. al. (2001) Biotechniques 30: 892-897). SMART prepared amplified cDNA was then normalized using DSN normalization method (Zhulidov et. al., (2004) Nucleic Acid Res. 32(3): e37. Normalization uncluded cDNA denaturation/reassociation, treatment of duplex specific nuclease (DSN) (Shagin et. al. (2002) Genome Res. 12: 1935-1942) and amplicification of normalized fraction by PCR. Resulted normalized cDNA was digested with SfiI and subcloned into pGADT7Rec-AB.

text: 1.2 to 3 month

text: Taraxacum kok-saghyz

text: SfiIA

text: SfiIB

text: root

text: Taraxacum kok-saghyz root cDNA library

text: Plasmids


text: Washington University School of Medicine

characteristic: INSDC first public

text: live

text: Generic

characteristic: NCBI submission model

text: Approximately 1.2 million independent transformants were amplified. Size selection during construction mainly excluded very small (i.e. less than 400nt) mRNA. The entire library was excised in pZL1 from Lambda ZipLox Phage. Y1090 (ZL) used for plaque plating and DH10B (ZIP) used to convert the phage into plasmids.

text: Mixed

text: DH10B (ZIP)

text: Caenorhabditis remanei

text: Not I

text: Sal I

text: Male-female, 50-50

text: C.remanei EST SB146

text: Lambda converted to plasmid

text: GIBCO/BRL/Life Technologies lambda zip/lox, converted to pZL1


text: Tsinghua University

text: live

text: Generic

characteristic: NCBI submission package

text: Pinctada fucata

text: mantle

text: mantle of Pinctada fucata subtraction cDNA library

text: pGEM-T Easy Vector


text: UMR CNRS-UPS 5546

text: live

text: Generic

text: Eucalyptus gunnii

text: differentiating xylem

text: Eucalyptus gunnii xylem versus phloem SSH library

text: Plasmid

text: pGEMT-easy


text: School of Agricultural Biotechnology, Seoul National University

text: live

text: Generic

text: The libraries were synthesized using the ZAP-cDNA synthesis method (Stratagene). cDNA was prepared, size-fractionated and inserted into the Uni-ZAP XR vector using XhoI linker-primer and EcoRI adaptor. After in vivo excision with E. coli strain SOLR, cDNA libraries from testis contain each insert size range of 0.5~3kb (n=20).

characteristic: description

text: 6.5 day embryo

text: Gallus gallus

text: XhoI

text: EcoRI

text: White Leghorn

text: Gonadal PGC

text: Embryonic gonadal PGC cDNA Library

text: phage

text: Uni-ZAP XR


text: National Cancer Institute / NIH

text: live

text: Generic

text: Genome Res. 2004 Oct;14(10B):2128-35.

text: DH10B TonA

characteristic: lab-host

text: mixed

characteristic: organ

text: Homo sapiens

text: DFCI-Vidal horfeome 1.1


text: National Institute of Agrobiological Sciences

text: live

text: Generic

text: Pyricularia grisea

characteristic: organism

characteristic: strain

text: mycelia

text: Magnaporthe grisea Guy11


text: Roslin Institute

text: live

text: Generic

text: sequence derived from either Bos taurus or Bos indicus. sequence provenance lost

text: Bos sp.

text: bone marrow

text: KN-252-bone-marrow, Bos sp.

characteristic: title


text: Roslin Institute

text: live

text: Generic

text: breed: N'dama

text: Bos taurus

text: bone marrow

text: KN-252-bone-marrow, Bos taurus


text: Roslin Institute

text: live

text: Generic

text: breed: Boran

text: Bos indicus

text: bone marrow

text: KN-252-bone-marrow, Bos indicus


text: Roslin Institute

text: live

text: Generic

text: sequence derived from either Bos taurus or Bos indicus; sequence provenance lost

text: Bos sp.

text: liver

text: KN-252-liver, Bos sp.


text: Roslin Institute

text: live

text: Generic

text: breed: N'dama

text: Bos taurus

text: liver

text: KN-252-liver, Bos taurus


text: Roslin Institute

text: live

text: Generic

text: breed: Boran

text: Bos indicus

text: liver

text: KN-252-liver, Bos indicus


text: Roslin Institute

text: live

text: Generic

text: Bos sp.

text: lymph node

text: KN-252-lymph, Bos sp.


text: Roslin Institute

text: live

text: Generic

text: breed: N'dama

text: Bos taurus

text: lymph node

text: KN-252-lymph, Bos taurus


text: Roslin Institute

text: live

text: Generic

text: breed: Boran

text: Bos indicus

text: lymph node

text: KN-252-lymph, Bos indicus


text: Roslin Institute

text: live

text: Generic

text: Bos sp.

text: spleen

text: KN-252-spleen, Bos sp.


text: Roslin Institute

text: live

text: Generic

text: breed: N'dama

text: Bos taurus

text: spleen

text: KN-252-spleen, Bos taurus


text: Roslin Institute

text: live

text: Generic

text: breed: Boran

text: Bos indicus

text: spleen

text: KN-252-spleen, Bos indicus


text: The Research Institute of Forestry, Chinese Academy of Forestry

text: live

text: Generic

text: Populus simonii

text: Populus simonii Carr


text: Southeast Missouri State University

text: live

text: Generic

text: Mycelia grown for three days at 37 degrees on minimal medium, then transferred to pre-warmed minimal media and incubated at 42 degrees for one hour before harvesting.

text: vegetative monokaryotic mycelium

text: E. coli XL10-Gold

text: Coprinopsis cinerea

text: EcoRI

text: XhoI

text: Coprinus cinereus heat-shocked mycelia cDNAs

text: phagemid

text: pBluescript II SK+

characteristic: vector


text: National Eye Institute

text: live

text: Generic

text: RNA was extracted from 9 pooled human pterygia. A directionally cloned cDNA library in the pCMVSPORT6 vector (Invitrogen) was constructed at Bioserve Biotechnology (Laurel MD) essentially following the protocols of the SuperScript Plasmid System, full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. cDNA was cloned in Not I/Sal I sites. EST analysis was performed at the NIH Intramural Sequencing Center (NISC). Analyzed data available through http://neibank.nei.nih.gov.

text: Adult

characteristic: dev-stage

text: Eye

text: Homo sapiens

text: Pterygium

text: Human pterygium. Unnormalized (nav)

text: Plasmid


text: National Eye Institute

characteristic: INSDC last update

text: live

text: Generic

text: RNA was extracted from pooled 15day post-hatched chicken eye. A directionally cloned cDNA library in the pCMVSPORT6 vector (Invitrogen) was constructed at Bioserve Biotechnology (Laurel MD) essentially following the protocols of the SuperScript Plasmid System, full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. cDNA was cloned in Not I/Sal I sites. EST analysis was performed at the NIH Intramural Sequencing Center (NISC). Analyzed data available through http://neibank.nei.nih.gov.

text: 15d post-hatched

text: Eye

text: Gallus gallus

text: Whole eye

text: Chicken eye (hatched). Unnormalized (naw)

text: Plasmid


text: National Eye Institute

text: live

characteristic: INSDC status

text: Generic

text: RNA was extracted from pooled 15day embryo chicken eye. A directionally cloned cDNA library in the pCMVSPORT6 vector (Invitrogen) was constructed at Bioserve Biotechnology (Laurel MD) essentially following the protocols of the SuperScript Plasmid System, full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. cDNA was cloned in Not I/Sal I sites. EST analysis was performed at the NIH Intramural Sequencing Center (NISC). Analyzed data available through http://neibank.nei.nih.gov.

text: 15d embryo

text: Eye

text: Gallus gallus

text: Whole eye

text: Chicken eye (embryo). Unnormalized (nax)

text: Plasmid


text: University of Saskatchewan and National Research Council Plant Biotechnology Institute

text: live

text: Generic

text: Triticum monococcum

text: Line 441 epidermal library


text: University of Sao Paulo

text: live

text: Generic

text: hemocytes

characteristic: cell type

text: -Pac vector.

text: adult

text: Acanthoscurria gomesiana

text: EcoRI

text: NotI

text: hemocytes

text: A. gomesiana hemocytes library

text: Phagemid


text: University of Sao Paulo

text: live

text: Generic

text: hemocytes

text: normalized cDNA library. first strand synthesis with NotI-dT(18) primer. cDNAs > 350 bp selected by chromatography (Bio-Gel A50). double-stranded cDNAs ligated to EcoRI blunt adapter, digested with NotI, size selected (> 350 bp) and cloned into pT7T3-Pac vector. single-stranded plasmids hybridized with cDNA inserts at low Cot (aprox 5), remaining single-stranded plasmids (normalized library) selected using a hydroxyapatite (HAP) column.

text: adult

text: Acanthoscurria gomesiana

text: EcoRI

text: NotI

text: hemocytes

characteristic: tissue-type

text: A. gomesiana hemocytes normalized library

text: Phagemid


characteristic: External Id

text: University of British Columbia

text: live

text: Generic

characteristic: cultivar

text: cDNA was prepared from 5 micrograms of mRNA and directionally ligated into the pBluescript II SK (+) XR vector using the pBluescript II XR cDNA Library Construction Kit according to manufacturer's instructions with modifications (Stratagene). Plasmid DNA then was transformed by electroporation into DH10B cells (Invitrogen) for propagation.

text: E. coli DH10B cells

text: Male cones

text: Picea glauca

text: EcoRI (5' end of cDNA)

text: XhoI (3' end of cDNA)

text: Hermaphrodite

characteristic: sex

text: Cones from mature trees harvested at Vernon, British Columbia on May 16th, 2001

text: pBluescript II SK (+) XR


text: University of British Columbia

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

characteristic: NCBI submission model

text: cDNA was prepared from 5 micrograms of mRNA and directionally ligated into the pBluescript II SK (+) XR vector using the pBluescript II XR cDNA Library Construction Kit according to manufacturer's instructions with modifications (Stratagene). Plasmid DNA was then transformed by electroporation into DH10B cells (Invitrogen) for propagation. Normalization was applied according to published methods [Bonaldo M.F. et al. (1996) Genome Research 6(9):791] in order to reduce the abundance of highly expressed transcripts.

text: E. coli DH10B cells

text: Male cones

text: Picea glauca

text: EcoRI (5' end of cDNA)

text: XhoI (3' end of cDNA)

text: Hermaphrodite

text: pBluescript II SK (+) XR


text: University of British Columbia

text: live

text: Generic

characteristic: NCBI submission package

text: Somatic embryo tissue was harvested at the callus stage, and after 2 weeks, 4 weeks and 6 weeks of growth on media supplemented with the growth regulators abscisic acid (ABA) and indole-3-butyric acid (IBA). mRNA was isolated from each tissue source independently and equal quantities of mRNA from each tissue were then pooled. cDNA was prepared from 5 micrograms of mRNA and directionally ligated into the pBluescript II SK (+) XR vector using the pBluescript II XR cDNA Library Construction Kit according to manufacturer's instructions with modifications (Stratagene). Plasmid DNA was then transformed by electroporation into DH10B cells (Invitrogen) for propagation.

characteristic: description

text: E. coli DH10B cells

characteristic: lab-host

text: Picea glauca

text: EcoRI (5' end of cDNA)

text: XhoI (3' end of cDNA)

text: Hermaphrodite

text: Somatic embryo tissue

text: pBluescript II SK (+) XR


text: University of British Columbia

text: live

text: Generic

text: Somatic embryo tissue was harvested at the callus stage, and after 2 weeks, 4 weeks and 6 weeks of growth on media supplemented with the growth regulators abscisic acid (ABA) and indole-3-butyric acid (IBA). mRNA was isolated from each tissue source independently and equal quantities of mRNA from each tissue were then pooled. cDNA was prepared from 5 micrograms of mRNA and directionally ligated into the pBluescript II SK (+) XR vector using the pBluescript II XR cDNA Library Construction Kit according to manufacturer's instructions with modifications (Stratagene). Plasmid DNA was then transformed by electroporation into DH10B cells (Invitrogen) for propagation. Normalization was applied according to published methods [Bonaldo M.F. et al. (1996) Genome Research 6(9):791] in order to reduce the abundance of highly expressed transcripts.

text: E. coli DH10B cells

text: Picea glauca

characteristic: organism

text: EcoRI (5' end of cDNA)

text: XhoI (3' end of cDNA)

text: Hermaphrodite

text: Somatic embryo tissue

text: pBluescript II SK (+) XR


text: Peking University

text: live

text: Generic

text: 5-10 day post anthesis

text: Gossypium hirsutum

text: EcoRI

text: XhoI

text: fiber

text: Cotton Lambda Zap Express Library

characteristic: title

text: plasmid

text: pBK-CMV


text: USDA

text: live

text: Generic

text: anamorph: Fusarium verticillioides. Mycelia were collected after growth in liquid GYAM (8 mM L-asparagine, 1.7 mM NaCl, 4.4 mM K2HPO4, 2 mM MgSO4, 8.8 mM CaCl2, 0.05% Yeast Extract, 0.24 M glucose and 5.0 mM malic acid) medium for 24 hours. Cultures were vacuum filtered and the mycelial mats were frozen in liquid nitrogen, ground to a powder, and then added to TRIzol Reagent (Invitrogen, Carlsbad CA) at approximately 1 g mycelia per 10 ml TRIzol. The cDNA was directionally ligated into the pBlueScript II SK(+) XR vector (cDNA Synthesis Kit; Stratagene).

text: Fusarium verticillioides

text: EcoRI

text: XhoI

characteristic: strain

text: mycelia

text: FvF

text: pBlueScript II SK(+) XR

characteristic: vector


text: USDA

characteristic: INSDC last update

text: live

text: Generic

text: anamorph: Fusarium verticillioides. Mycelia was collected after growth in liquid GYAM medium for 96 hours. Cultures were vacuum filtered and the mycelial mats were frozen in liquid nitrogen, ground to a powder, and then added to TRIzol Reagent (Invitrogen, Carlsbad CA) at approximately 1 g mycelia per 10 ml TRIzol. The cDNA was directionally ligated into the pBlueScript II SK(+) XR vector (cDNA Synthesis Kit; Stratagene).

text: Fusarium verticillioides

text: EcoRI

text: XhoI

text: mycelia

text: FvG

text: pBlueScript II SK(+) XR


text: USDA

text: live

characteristic: INSDC status

text: Generic

text: anamorph: Fusarium verticillioides. Library FvH was prepared from germinating conidia in an aqueous extract of maize seedlings. The aqueous extract was obtained by germinating surface-sterilized maize seeds for four days on 1% water agar supplemented with 100 ug/ml streptomycin sulfate. Under sterile conditions, 70 g of excised roots and shoots from the resulting seedlings was blended with 250 ml water, filtered once through MiraclothÂ® (Calbiochem) and then again through #1 Whatman filter paper. The extracts were inoculated to a spore concentration of 5 x 106 conidia per ml and then incubated for 10 hours at 30C with shaking at 200 RPM. Cultures were vacuum filtered and the mycelial mats were frozen in liquid nitrogen, ground to a powder, and then added to TRIzol Reagent (Invitrogen, Carlsbad CA) at approximately 1 g mycelia per 10 ml TRIzol. The cDNA was directionally ligated into the pBlueScript II SK(+) XR vector (cDNA Synthesis Kit; Stratagene).

text: Fusarium verticillioides

text: EcoRI

text: XhoI

text: mycelia

text: FvH

text: pBlueScript II SK(+) XR


text: USDA

text: live

text: Generic

text: anamorph: Fusarium verticillioides. Library FvI was prepared from growth on excised maize seedling roots and shoots. The roots and shoots were prepared as described above and then inoculated by dipping briefly in a suspension of 5 x 10e6 conidia per ml. The inoculated roots and shoots were then incubated on moistened Whatman #1 filter paper at room temperature under sterile conditions for 4 days. Fungal mycelial was collected, frozen in liquid nitrogen, ground to a powder, and then added to TRIzol Reagent (Invitrogen, Carlsbad CA) at approximately 1 g mycelia per 10 ml TRIzol. The cDNA was directionally ligated into the pBlueScript II SK(+) XR vector (cDNA Synthesis Kit; Stratagene).

text: Fusarium verticillioides

text: EcoRI

text: XhoI

text: mycelia

text: FvI

text: pBlueScript II SK(+) XR


text: USDA

text: live

text: Generic

text: anamorph: Fusarium verticillioides. Library FvJ was prepared from growth on infected ears of corn. The fungal/corn material was collected, frozen in liquid nitrogen, ground to a powder, and then added to TRIzol Reagent (Invitrogen, Carlsbad CA) at approximately 1 g mycelia per 10 ml TRIzol. The cDNA was directionally ligated into the pBlueScript II SK(+) XR vector (cDNA Synthesis Kit; Stratagene).

text: Fusarium verticillioides

text: EcoRI

text: XhoI

text: mycelia

text: FvJ

text: pBlueScript II SK(+) XR


text: USDA

text: live

text: Generic

text: anamorph: Fusarium verticillioides. Library FvK were constructed employing suppression subtractive hybridization (SSH) based on the PCR Select cDNA Subtraction Kit (Clonetech, USA). These libraries were created to specifically identify F. verticillioides genes up-regulated in response to maize antimicrobial defense compounds.

text: Fusarium verticillioides

text: EcoRI

text: XhoI

text: mycelia

text: FvK

text: pBlueScript II SK(+) XR


text: USDA

text: live

text: Generic

text: anamorph: Fusarium verticillioides. Library FvL were constructed employing suppression subtractive hybridization (SSH) based on the PCR Select cDNA Subtraction Kit (Clonetech, USA). These libraries were created to specifically identify F. verticillioides genes up-regulated in response to maize antimicrobial defense compounds.

text: Fusarium verticillioides

text: EcoRI

text: XhoI

text: mycelia

text: FvL

text: pBlueScript II SK(+) XR


text: USDA

text: live

text: Generic

text: anamorph: Fusarium verticillioides. Library FvM was prepared from pooled RNA obtained from a 48-hour and a 72 hour, liquid GYAM culture from strain M-3125. Cultures were vacuum filtered and the mycelial mats were frozen in liquid nitrogen, ground to a powder, and then added to TRIzol Reagent (Invitrogen, Carlsbad CA) at approximately 1 g mycelia per 10 ml TRIzol. The cDNA was directionally ligated into the pBlueScript II SK(+) XR vector (cDNA Synthesis Kit; Stratagene).

text: Fusarium verticillioides

text: EcoRI

text: XhoI

text: mycelia

text: FvM

text: pBlueScript II SK(+) XR


text: USDA

text: live

text: Generic

text: anamorph: Fusarium verticillioides. Library FvN was obtained from RNA derived from a corn meal medium culture of strain M-3125. These cultures were prepared by inoculating an autoclaved mixture of 25 g corn meal and 5 ml distilled water with 5 ml of water containing 5 x 10e7 condia. The inoculated medium was mixed thoroughly, distributed equally into two 100-mm plastic petri dishes, and incubated at room temperature for four or six days.

text: Fusarium verticillioides

text: EcoRI

text: XhoI

text: mycelia

text: FvN

text: pBlueScript II SK(+) XR


text: USDA

text: live

text: Generic

text: anamorph: Fusarium verticillioides. Library FvO was prepared from a 90-hour, liquid GYAM culture from the fumonisin-nonproducing strain 57-7-7. Cultures were vacuum filtered and the mycelial mats were frozen in liquid nitrogen, ground to a powder, and then added to TRIzol Reagent (Invitrogen, Carlsbad CA) at approximately 1 g mycelia per 10 ml TRIzol. The cDNA was directionally ligated into the pBlueScript II SK(+) XR vector (cDNA Synthesis Kit; Stratagene).

text: Fusarium verticillioides

text: EcoRI

text: XhoI

text: mycelia

text: FvO

text: pBlueScript II SK(+) XR


text: Berry College

text: live

text: Generic

text: Expression induced by 4 hr exposure to 50.0 ug/L copper

text: Adult

characteristic: dev-stage

text: Acropora cervicornis

text: Coral stress responses, copper exposure


text: Nova Scotia Agricultural College

text: live

text: Generic

text: Russet Burbank

text: Solanum tuberosum

text: Solanum tuberosum cv. Russet Burbank


text: Cotton Research Institute, Chinese Academy of Agricultural Sciences

text: live

text: Generic

text: genotype: (AD)2

text: one month after subculture

text: Gossypium hirsutum

text: embryoid

characteristic: tissue-type

text: Gossypium hirsutum (AD)2 embryoid one month after subculture


characteristic: External Id

text: live

text: Generic

text: one month after subculture

text: Gossypium hirsutum

text: embryoid

text: Gossypium hirsutum embryoid one month after subculture


text: live

text: Generic

characteristic: cultivar

text: one month after subculture

text: Gossypium hirsutum

text: embryoid

text: Gossypium hirsutum cv. TM-1 embryoid one month after subculture


text: USDA/ARS

text: live

text: Generic

text: Egg cells were isolated with enzymatic maceration and manual microdissection. RNA was extracted from the isolated egg cells. The cDNA library was constructed by oligo dT priming using the Clontech SMART cDNA synthesis kit. The cDNAs were directionally cloned.

text: Zea mays

text: Zea mays egg cell cDNA library

text: Clontech Triplex2


text: NRC Institute for Marine Biosciences

text: live

text: Generic

text: enriched macrophages

characteristic: cell type

text: Library (SM1) made by SMART cDNA synthesis

text: adult

text: Salmo salar

text: Atlantic Salmon macrophage


text: NRC Institute for Marine Biosciences

characteristic: INSDC center name

text: live

text: Generic

text: enriched macrophages

text: Libraries (SMT2, SMT3, SMT6, SMT7) made by forward and reverse suppression subtractive hybridization using salmon macrophage infected in vitro for 0.5h (SMT2 and SMT3) or 2h (SMT6 and SMT7) with Aeromonas salmonicida cultured in broth vs. uninfected macrophages

text: adult

text: Salmo salar

text: Atlantic Salmon macrophage - Aeromonas in vitro infection

text: pT-Adv


text: NRC Institute for Marine Biosciences

characteristic: INSDC first public

text: live

text: Generic

text: enriched macrophages

text: Libraries (SMV2, SMV3, SMV6, SMV7) made by forward and reverse suppression subtractive hybridization using salmon macrophage infected in vitro for 0.5h (SMV2 and SMV3) or 2h (SMV6 and SMV7) with Aeromonas salmonicida cultured in the intraperitoneal space of fish vs. uninfected macrophages

text: adult

text: Salmo salar

text: Atlantic Salmon macrophage - Aeromonas in vivo infection

text: pT-Adv


text: University of Arizona

text: live

text: Generic

characteristic: NCBI submission model

text: Direct sequencing of Arabidopsis thaliana RT-PCR products

text: 7 to 10 day old

text: Arabidopsis thaliana

text: Ecotype Columbia

text: Seedlings

text: Arabidopsis thaliana RT-PCR products


text: National Cancer Institute / NIH

text: live

text: Generic

characteristic: NCBI submission package

text: cDNA was primed using oligo-dT primer: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3' and cloned into the EcoRV/NotI sites of pExpress-1. Size-selection 1.4kb resulted in an average insert size of 2.1kb. This is a non-normalized primary library (normalized library is NICHD_XGC_Emb10) and was constructed by Express Genomics (Frederick, MD). Note: this is a Xenopus Gene Collection (http://xgc.nci.nih.gov/) library.

text: DH10B TonA

text: embryo

characteristic: organ

text: Xenopus laevis

text: EcoRV

text: NotI

text: Embryonic stage 17/19

text: plasmid


text: National Cancer Institute / NIH

text: live

text: Generic

text: cDNA was primed using oligo-dT primer: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3' and cloned into the EcoRV/NotI sites of pExpress-1. Size-selection 1.4kb resulted in an average insert size of 1.8kb. This is a normalized library (primary library is NICHD_XGC_Emb9) and was constructed by Express Genomics (Frederick, MD). Note: this is a Xenopus Gene Collection (http://xgc.nci.nih.gov/) library.

characteristic: description

text: DH10B TonA

characteristic: lab-host

text: embryo

text: Xenopus laevis

text: EcoRV

text: NotI

text: Embryonic stage 17/19

text: plasmid


text: Agriculture and Agri-Food Canada

text: live

text: Generic

text: N-o-9 (Sharpe et al (1995) Genome 38: 1112-1121)

text: cDNA was generated from etiolated B. napus seedlings, 5 days after germination. The cDNA was directionally cloned into the EcoRI and XhoI sites of Stratagene's Lambda ZAP II vector. cDNA clones were then excised into the pBluescript phagemid vector.

text: Brassica napus var. napus

characteristic: organism

text: Etiolated seedling

text: Brassica napus lambda ZAP II library

characteristic: title

text: Plasmid

text: lambda ZAP II


text: Concordia University

text: live

text: Generic

text: Complementary DNA was synthesized with ZAP kit (Stratagene) using poly(A)+RNA isolated from Aspergillus niger grown in both complete and minimal media. Synthesis was primed with oligo(dT)/XhoI primer. EcoRI adaptors were ligated to the blunt-ended, double-stranded cDNA. EcoRI-XhoI-digested cDNA was ligated with EcoRI-XhoI-digested pYES2 (Invitrogen Corp). This vector permits expression of our library in yeast.

text: mycelial growth

text: E. coli

text: Aspergillus niger

text: XhoI

text: EcoRI

characteristic: strain

text: Aspergillus niger pYES2 (XhoI-EcoRI)

text: plasmid

characteristic: vector


text: Concordia University

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Complementary DNA was synthesized with ZAP kit (Stratagene) using poly(A)+RNA isolated from Aspergillus niger cultured under different carbon sources (glucose, maltose, xylose, lactose, sorbitol, xylan, and bran). Synthesis was primed with oligo(dT)/XhoI primer. EcoRI adaptors were ligated to the bunt-ended, double-stranded cDNA. The EcoRI-XhoI-digested cDNA was ligated with EcoRI-XhoI-digested pBluescript KS+ (Invitrogen Corp).

text: mycelial growth

text: E. coli

text: Aspergillus niger

text: XhoI

text: EcoRI

text: Aspergillus niger pBluescript (EcoRI-XhoI)

text: plasmid

text: pBluescript KS+


text: USDA-ARS

text: live

text: Generic

text: Perillus bioculatus used in this investigation are from colonies maintained at Biological Control of Insects Research Laboratory (Columbia, MO). Insects were reared in walk-in chambers set at 14 h light: 10 h dark, 26 +/- 2 oC and 65 +/- 10% relative humidity. Trichoplusia ni is used as the primary host for maintaining the laboratory colonies. The artificial diet used in this investigation is a blended mixture of a plant meridic diet to which beef liver and whole egg are added (Coudron et al., 2002, Journal of Economic Entomology 95, 1159-1168). Total RNA was isolated as described above from 3rd instar nymphs fed the artificial diet for 15 generations or prey. Forward and reverse subtracted libraries were constructed using the Clontech PCR-Select kit. The libraries were cloned into pCR2.1 and 1152 clones were isolated. An aliquot from each culture was transferred onto positively charged nylon membrane using a 96 pin MULTI-PRINT applicator. The filters were screened with randomly primed probes generated from 100 ng of the unsubtracted libraries cDNA to identify clones of differentially expressed genes.

text: Third instar nymphs

characteristic: dev-stage

text: Perillus bioculatus

text: Perillus bioculatus diet SSH library

text: plasmid


characteristic: External Id

text: Universita degli Studi dell'Insubria

text: live

text: Generic

text: Perca fluviatilis

text: adult liver

characteristic: tissue-type

text: P. fluviatilis liver cDNA library


text: Dalhousie University

text: live

text: Generic

text: Seculamonas ecuadoriensis


text: Dalhousie University

text: live

text: Generic

text: Jakoba bahamiensis


text: Dalhousie University

text: live

text: Generic

text: Malawimonas jakobiformis


text: Dalhousie University

text: live

text: Generic

text: Prototheca wickerhamii


text: Central Institute of Medicinal and Aromatic Plants

text: live

text: Generic

text: Whole placenta

characteristic: cell type

text: cDNA populations derived from the total mRNA of highly pungent fruit placenta tissues of Capsicum frutescens L. The fruit placenta specific messages were cloned in the lambda ZAP express vector.

text: 30 days after flowering

text: E coli

text: Fruit

text: Capsicum frutescens

text: EcoR I

text: Xho I

text: Hermaphrodite

characteristic: sex

text: Placenta

text: Capsicum frutescens L. fruit placenta specific cDNA library

text: Phagemid

text: pBK CMV


text: The University of Georgia, Department of Plant Biology

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: The library was prepared from polyA+ RNA from the roots of 1-year-old loblolly pine (Pinus taeda) cuttings that were rooted and then planted in washed sand. The rooted cuttings were maintained for 143 days (August 1 2003 harvest) under ambient conditions in a local greenhouse. They were kept on a weekly regimen of 0.5x nutrient-complete Hoagland's solution and supplemented with additional water sufficient to maintain a 15% soil moisture content. Twenty-four hours (24h) prior to harvesting roots for mRNA preparation, the potted trees were watered with 50 uM CuSO4 (aq) until the sand was saturated. Double-stranded cDNA was cloned unidirectionally into pSL1180. Inserts can be excised with EcoRI (5' end) and XhoI (3' end).

text: DH10B-T1 phage-resistant E. coli

text: Root

text: Pinus taeda

text: EcoRI

text: XhoI

text: 3 CCLONES

text: Roots plus added copper


text: Institute of Hygiene and Microbiology

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Echinococcus multilocularis is a cestode parasite of foxes and dogs (definitive hosts) and various species of small rodents as well as humans (intermediate hosts). The library was constructed from parasite larvae (metacestode and protoscolex) after cultivation in laboratory animals (gerbils; Meriones unguiculatus). cDNA was prepared by RT, primed with a tagged oligo-dT (XhoI primer adapter). After first and second strand cDNA synthesis, a second adapter (EcoRI) was added to the 5' ends. The cDNA was then directionally cloned into pJG4-5 cut with EcoRI and XhoI. At the 3' ends of the cDNAs, immediately following the polyA, an XhoI site (CTC GAG) has been introduced. At the 5' ends, a linke that introduced an EcoRI site has been added (sequence: 5'-GAA TTC GGC ACG AG-3'). The XhoI site and the linker are not part of the natural mRNAs.

text: Larva (protoscolex and metacestode)

text: Echinococcus multilocularis

text: EcoRI

text: XhoI

text: Mixed

text: Echinococcus multilocularis larvae (yeast two-hybrid)

text: Plasmid


text: Institute of Hygiene and Microbiology

text: live

text: Generic

text: Echinococcus multilocularis is a cestode parasite of foxes and dogs (definitive hosts) and various species of small rodents as well as humans (intermediate hosts). The library was constructed from protoscoleces obtained after cultivation in laboratory animals (gerbils; Meriones unguiculatus). cDNA was prepared by RT-PCR and non-directionally cloned into a plasmid vector. RT was primed with a tagged oligo-dT (primer CD3-RT). After first strand cDNA synthesis, PCR was performed using primers CD3, directed against the 3' end, and primer EM10SL, directed against the E. multilocularis spliced leader. The cDNA was then non-directionally cloned into pCR2.1TOPO using TOPO-TA cloning. The part of the spliced leader sequence that is present at 5' ends reads: 5'-TCC TTA CCT TGC AGT TTT GTA TG-3'. This sequence is natural part of the mRNA molecules. At the 3' ends, between the polyA-part and the vector, the sequence 5'-TGG CCG CAT GCC GAC TAG TAC-3' has been added as tagging sequence during reverse transcription. This sequence does not form part of the natural mRNA molecules.

characteristic: description

text: Larva (Protoscolex)

text: Echinococcus multilocularis

text: EcoRV

text: Mixed

text: Echinococcus multilocularis protoscolex (spliced leader)

text: Plasmid


text: National Chemical Laboratory

text: live

text: Generic

characteristic: cultivar

text: This sequence was obtained by cDNA-AFLP where cDNAs were synthesized from reverse transcription of mRNA isolated from chickpea roots at different days after infection with Fusarium oxysporum f. sp. ciceri Race 1. Three cDNA libraries were made using Zap-cDNA synthesis kit Gigapack III cloning Kit from Stratagene, 11011 North Torrey Pines Road, La Jolla, CA 92037; 1) infected ressitant chickpea cultivar WR 315; 2) un-infected control of WR 315, 3) infected susceptable chickpea cultivar JG 62. cDNAs from the libraries were rescued by PCR amplification using flanking T3 and T7 promoter primers. Amplified cDNA was digested with EcoRI and MseI restriction endonucleases simultaneously. Subsequently,the cDNA fragments were ligated to EcoRI and MseI adapters to generate template DNA for amplification. These common adapter sequences flanking variable cDNA sequences serve as the primer binding sites on these restriction fragments. Small DNA fragments were generated, then amplified by PCR using different primer combinations including two selective bases in the presence of 33P for radioactive labeling of the amplified fragments. The PCR products from all the three cDNA libraries were run on a denaturing polyacrylamide gel. The up-regulated bands from resistant infected library were isolated from the gel, reamplified using the same primers and cloned in pGEMT easy vector (Promega) and sequenced in one direction using the T7 or SP6 primer.

text: chickpea roots Post infection with Fusarium oxysporum f. sp. ciceri race 1

characteristic: lab-host

text: Root

characteristic: organ

text: Cicer arietinum

text: EcoRI

text: EcoRI

text: infected root

text: cDNA-AFLP Chickpea roots challenged by Fusarium oxysporum f. sp. ciceri Race 1

text: Plasmid

text: pGEMT


text: National Chemical Laboratory

text: live

text: Generic

text: This sequence was obtained by cDNA-RAPD where cDNAs were synthesized from reverse transcription of mRNA isolated from chickpea roots at different days after infection with Fusarium oxysporum f. sp. ciceri Race 1. Three cDNA libraries were made using Zap-cDNA synthesis kit Gigapack III cloning Kit from Stratagene, 11011 North Torrey Pines Road, La Jolla, CA 92037; 1) infected ressitant chickpea cultivar WR 315; 2) un-infected control of WR 315, 3) infected susceptable chickpea cultivar JG 62. cDNAs from the libraries were rescued by PCR amplification using flanking T3 and T7 promoter primers. Amplified cDNA was used as template to screen with RAPD primers from UBC. Small DNA fragments were generated, then amplified by PCR using different RAPD (hexamers) primers. The PCR products from all the three cDNA libraries were run on a 1.5% Agarose gel. The up-regulated bands form resistant infected library were isolated from the gel, reamplified using the same primers and cloned in pGEMT easy vector (Promega) and sequenced in both direction using the T7 and SP6 primer.

text: Root

text: Cicer arietinum

characteristic: organism

text: EcoRI

text: EcoRI

text: infected root

text: cDNA-RAPD Chickpea roots challenged by Fusarium oxysporum f. sp. ciceri Race 1

characteristic: title

text: Plasmid

text: pGEMT


text: State Research Institute Geisenheim

characteristic: INSDC last update

text: live

text: Generic

text: Boerner

text: The cultivar 'Boerner' is a cross breeding: Vitis riparia 183 Geisenheim x Vitis cinerea Arnold

text: adult plants

text: root

text: Vitis riparia x Vitis cinerea

text: EcoRI

text: SpeI

text: male

text: root tip tissue (in vitro culture)

text: Boerner cDNA library

text: Plasmid

text: pGEM-Teasy Vector

characteristic: vector


text: Bielefeld University, Institute for Genome Research

text: live

characteristic: INSDC status

text: Generic

text: Columbia

text: In the context of the EU-funded project REGIA (QLG-CT11999-00876, coordinator Javier Paz-Ares), a set of transcription factor ORFs was generated. The ORFs were produced in a decentralized way in the labs of the participants. Most of the ORFs were generated by RT-PCR using cDNA from various A. thaliana tissues as a template. Initially, it was planned to use yeast recombination to move the ORFs from the cloning vectors into target constructs. For this reason, a number of the clones contain 'REGIA tags' (RG_tag1: 5pr-AATTCCAGCTCACCACC-3pr; RG_tag2: 5pr-CATGGCAATTCCCGGGGATC-3pr). During the lifetime of the project, the GATEWAY system became available and finally all ORFs were transferred into GATEWAY vectors. At the end of the REGIA project, the clones were collected as plasmid DNA. For end-sequencing at the DNA core facility of the MPI of Plant Breeding Research (ADIS; head: Bernd Weisshaar), the plasmids were re-transformed into DH5alpha, arrayed into 96-well plates and new plasmid DNA was prepared. Re-transformation proved to be difficult because a number of clones displayed poor growth. Data submission has been handled by GabiPD (http://gabi.rzpd.de).

text: E. coli DH5alpha

text: Arabidopsis thaliana


characteristic: External Id

text: live

text: Generic

text: Columbia

text: In the context of the GABI-TFs project (PI: Bernd Weisshaar; GABI-TFs was a part of the German plant genomics program GABI, FKZ 0312276N), a set of transcription factor ORFs was generated. The ORFs were generated by RT-PCR using cDNA from various A. thaliana tissues as a template, transferred into an GATEWAY vector and combined with complementary transcription factor ORFs from the REGIA project. For end-sequencing at the DNA core facility of the MPI of Plant Breeding Research (ADIS; head: Bernd Weisshaar), the clones were arrayed into 96-well plates and plasmid DNA was prepared. Data submission has been handled by RZPD. This clone is available from RZPD; please go to 'http://www.rzpd.de/about/contact/' for further information.

text: E. coli DH5alpha

text: Arabidopsis thaliana


text: live

text: Generic

text: Columbia

text: E. coli DH5alpha

text: Arabidopsis thaliana

text: assumed to be pDONR201


text: Huazhong Agricultural University

text: live

text: Generic

text: Detached leaves were challenged with a suspension of P. infestans zoospores at 1 x 105/mL. subsequently, the infected samples were harvested at 48 h after inoculation, and then snap-frozen in liquid nitrogen. Control samples were sprayed with water.

text: 8 weeks old

characteristic: dev-stage

text: Solanum tuberosum

text: SmalI

text: NotI

text: potato clone, 386209.10

characteristic: strain

text: leaf

characteristic: tissue-type

text: suppression subtractive hybridization library of Phytophthora infestans-challenged leaves

text: pBluescript SK (+)


text: Virginia Polytechnic Institute and State University

characteristic: INSDC center name

text: live

text: Generic

text: Burley 21

text: TA-cloning

text: Nicotiana tabacum

text: Primary root cultures

text: Fluorescent differential display amplification products

text: Plasmid

text: pGEM-T


text: Kansas State University

characteristic: INSDC first public

text: live

text: Generic

text: Century and Atlas 66

text: Triticum aestivum

text: Roots

text: Wheat Aluminum SSH Library


text: Kansas State University

text: live

text: Generic

characteristic: NCBI submission model

text: Ning 7840 and Clark

text: Triticum aestivum

text: F. graminearum-point-inoculated spike

text: Wheat FHB SSH Library


text: Southeast Missouri State University

text: live

text: Generic

characteristic: NCBI submission package

text: Mycelia grown for three days at 37 degrees on YMG medium.

text: vegetative monokaryotic mycelium

text: E. coli XL10-Gold

text: Coprinopsis cinerea

text: EcoRI

text: XhoI

text: Coprinus cinereus YMG medium exponentially growing mycelia cDNAs

text: phagemid

text: pBluescript II SK+


text: Southeast Missouri State University

text: live

text: Generic

text: Mycelia grown for three days at 37 degrees on medium containing 2% (w/v) cellobiose and 0.4% (w/v) gelatin as carbon and nitrogen sources.

characteristic: description

text: vegetative monokaryotic mycelium

text: E. coli XL10-Gold

text: Coprinopsis cinerea

text: EcoRI

text: XhoI

text: Coprinus cinereus complex C/N source medium exponentially growing mycelia cDNAs

text: phagemid

text: pBluescript II SK+


text: Institut fur Genetik, Universitat zu Koln

text: live

text: Generic

text: 3'-RACE products cloned in pCR4-TOPO vector (Invitrogen).

text: Mixed stages

text: Tribolium castaneum

text: Wild type

text: Tribolium castaneum Cloned RACE products

text: Plasmid


text: CEINGE Biotecnologie Avanzate S.C.a r.l.

text: live

text: Generic

text: embryonic day E14.5 of gestation

text: Mus musculus

text: telencephalon

text: Telencephalic Embryonic Sutractive Sequences


text: ETH

text: live

text: Generic

characteristic: cultivar

text: Suppression Subtractive Hybridization (SSH) was used to isolate large numbers of low temperature-induced genes from cold-treated maize (Zea mays L.). Maize plants were exposed to 6oC for 48 hours.

text: Zea mays

text: Zea mays cold-treated


text: USDA - ARS

text: live

text: Generic

text: A high quality EST with at least 100 contiguous bases at Trace Tuner score of 20 or better. Library construction by L.E. Hunnicutt (USDA-ARS-USHRL).

text: Adult

characteristic: lab-host

text: Oncometopia nigricans

text: EcoRI

text: XhoI

text: Mixed population

characteristic: sex

text: Whole body

text: adult black-winged sharpshooter

text: pBluescript II SK+


text: University of Alberta

text: live

text: Generic

text: adult

text: Anas platyrhynchos

characteristic: organism

text: EcoRI

text: XhoI

text: male

text: White Pekin duck

text: spleen

text: Duck spleen ZAP Express cDNA Library

text: phagemid

text: pBK-CMV


text: University of Washington

text: live

text: Generic

text: spleen

characteristic: organ

text: Haemorhous mexicanus

text: House Finch spleen SSH cDNA Library

characteristic: title


text: Sun Yat-sen University

characteristic: INSDC last update

text: live

text: Generic

text: Jinnan

text: Medicago sativa

text: callus

text: Alfalfa callus Forward subtracted library

text: Plasmid

text: pGEM T-Easy

characteristic: vector


characteristic: External Id

text: Agricultural research organization, Newe Yaiar research center

text: live

characteristic: INSDC status

text: Generic

text: Noy Yizre'el

text: Four SSH libraries were developed from melon fruit. Subtractions were performed reciprocally between 25 Days Post Anthesis (DPA) (young) and 47 DPA (mature) fruits and separately for the fruit flesh and for the fruit rind.

text: Young (25 Days Post Anthesis (DPA)) and mature (47 DPA) fruits.

text: E.coli JM109 competent cells

text: fruit

text: Cucumis melo

text: fruit flesh or fruit rind

text: Mature/Young fruit SSH library

text: Plasmid

text: pGEM-T Easy


text: Beijing Genomics Institute

text: live

text: Generic

text: B cell

characteristic: cell type

text: Macaca fascicularis

text: Macaca fascicularis B cell cDNA library


text: St James's University Hospital

text: live

text: Generic

text: urothelial

text: Bos taurus

text: Bos taurus urothelial cells


text: University of California

text: live

text: Generic

text: Phytophthora infestans

text: SalI

text: NotI

characteristic: strain

text: Hyphae

text: Plasmid


text: The University of Arizona

characteristic: INSDC center name

text: live

text: Generic

text: Maize Full length cDNA library (3530 library) created by Invitrogen from multiple tissues; Organ: silks, husks, ears, pollen, shoot tips, leaf, root tips, whole seed, embryo. This is a Gateway compatible vector, permitting clone movement to new vector backbones for expression in diverse host cells using recombination rather than restriction enzymes. poly(A)+ mRNA was prepared by Invitrogen, and equimolar amounts of RNA from each of the 12 tissue samples were mixed together for selection of mRNA with a 5' cap. After synthesis of cDNA, a normalization step was conducted against the mixture of RNA sources. Tissues prepared: 1. just emerging silks; 2. inner husks from ears of sample #1; 3. 20 dap aleurone; 4. immature tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day old seedlings; all leaves with an expanded or partially expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 cm long root tips from 15 day old seedlings; 10. 10 dap whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap endosperm and embryo. All of the sequenced clones in Maize Full-length cDNA Project will be archived at the University of Arizona. Clones, high density filters and amplified library can be ordered from the University of Arizona (http://www.genome.arizona.edu/orders/).

text: varies by tissue

characteristic: dev-stage

text: DH10B T1 phage resistant

text: Zea mays

text: EcoRV

text: NotI

text: mixed (silks, husks, ears, pollen, shoot tips, leaf, root tips, whole seed, embryo)

characteristic: tissue-type

text: Plasmid

text: pCMV-SPORT 6.1


text: Bangalore University

characteristic: INSDC first public

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: partial sequence obtained by DDRT PCR from drought induced leaves of peanut seedlings

text: seedling

text: Arachis hypogaea

text: drought-stressed leaves

text: Arachis hypogaea drought-stressed leaves seedling


text: UMR INRA INSA Lyon

text: live

text: Generic

text: strain nm1

characteristic: cultivar

text: Sample name: AGGUT1 Plant growth place: Lyon, UMR INRA INSA de Lyon, greenhouse. Description: Dissected aphid guts, mRNA prepared and substracted with bulk RNA from non-digestive tissue (same dissection). experimental condition: normal, grown on cucumis melo cv. vedrantais

characteristic: description

text: adult

text: Ecoli DH10B, Life-Technologies / Invitrogen

characteristic: lab-host

text: Aphis gossypii

text: EcoRV

text: gut, digestive tract (whole midgut and hindgut)

text: pGEM-T easy, from pGEM 5ZF+


text: College of Marine Life Sciences, Ocean University of China

text: live

text: Generic

text: Sporophyte

text: Neopyropia yezoensis

characteristic: organism

text: myx cDNA Library


text: Southeast Missouri State University

text: live

text: Generic

text: Mycelia were grown on minimal medium for 9 days. The first four days' growth was harvested.

text: vegetative monokaryotic mycelium

text: E. coli XL10-Gold

text: Coprinopsis cinerea

text: EcoRI

text: XhoI

text: Coprinus cinereus senescent mycelia cDNAs

characteristic: title

text: phagemid

text: pBluescript II SK+


text: USDA - ARS

characteristic: INSDC last update

text: live

text: Generic

text: Valencia

text: Mature tree 3 inch caliber

text: Citrus sinensis

text: EcoRI

text: XhoI

text: phloem

text: Citrus sinensis phloem

text: pBluescript II SK+


text: University of California, Santa Barbara

text: live

text: Generic

text: F2, F3, and F4 lines of Aquilegia formosa X A. pubescens were grown from seed in greenhouses at UC Santa Barbara. From these plants three sets of tissue were collected: 1) Small flower buds (<10 mm) and very young inflorescences (71 & 29% by weight respectively), 2) Medium (7-20 mm) and large (at or near anthesis) flower buds (65 & 35% by weight respectively) and 3) Shoot apical meristems. A fourth set of tissue was collected from plants of A. formosa. These plants were grown from seed in sand and at approximately 1 month root tissue and leaf tissue of various developmental stages were collected (84 & 16% by weight respectively). Total RNA was extracted from each set of tissue and pooled in the following proportions: 1.5X from sets 1 & 2, 1X from sets 3 & 4. From the pooled total RNA, mRNA was extracted and enriched for full-length messages and then normalized with proprietary methods by Invitrogen.

text: DH10B T1 (T1 and T5 phage resistance)

text: Aquilegia formosa x Aquilegia pubescens

text: EcoRI

text: NotI

text: mixed shoot and floral apical meristems, flower buds, leaves and roots

text: Aquilegia cDNA library

text: pCMV SPORT6.1

characteristic: vector


characteristic: External Id

text: Washington University School of Medicine

text: live

text: Generic

text: 1st strand of cDNA was synthesized with reverse transcriptase and oligo(dT) beads, then cDNA was amplified by PCR using modified SMART primers. The final cDNA was cloned in pAMP1 vector in annealing reaction with Uracil DNA Glycosylase (UDG).

text: Gallus gallus

text: Primary Chick Utricle Subtraction

text: bacterial


text: Agricultural research organization, Newe Yaiar research center

text: live

characteristic: INSDC status

text: Generic

text: En Dor

text: Suppression Subtractive Hybridization (SSH) libraries were developed from melon seedlings treated with trifluralin herbicide and Fusarium Oxysporum f. sp. Melonis (FOM) fungus. Subtractions were performed between the trifluralin & FOM treated seedlings' cDNA (tester) and untreated seedlings' cDNA (driver).

text: thirteen day old seedlings treated with trifluralin herbicide and FOM fungus

text: E. coli JM109 competent cells

text: Cucumis melo

text: Root and Stem

text: SSH1 melon SSH Library

text: Plasmid

text: pGEM-T Easy


text: live

text: Generic

text: En Dor

text: E. coli JM109 competent cells

text: Cucumis melo

text: cotyledon

text: SSH5 melon SSH Library

text: Plasmid

text: pGEM-T Easy


text: live

text: Generic

text: En Dor

text: The cDNA-AFLP technique was applied using AFLP analysis System I (GIBCO BRL) kit. The cDNA sequences exhibited differential expression following treatments with trifluralin herbicide and Fusarium Oxysporum f. sp. Melonis (FOM) fungus.

text: Ten or thirteen day old seedlings treated with trifluralin herbicide and FOM fungus

text: E. coli JM109 competent cells

text: Cucumis melo

text: either cotyledon or Stem and root

text: melon cDNA-AFLP Library

text: Plasmid

text: pGEM-T Easy


text: PTP-CERSA

text: live

text: Generic

text: skin

characteristic: organ

text: Acipenser transmontanus

text: A. transmontanus skin express library


text: PTP-CERSA

text: live

text: Generic

text: spleen

text: Acipenser transmontanus

text: A. transmontanus spleen express library


text: University of Chicago Medical Center

text: live

text: Generic

text: The 3' EST was isolated by a SAGE tag CATG- through mis-priming using the GLGI technique.

text: Homo sapiens

text: Female

characteristic: sex

text: Brain

text: Brain 3' EST

text: Plasmid

text: pGEM-T


text: ENH Research Institute, Feinberg School of Medicine, Northwestern University

characteristic: INSDC center name

text: live

text: Generic

text: Homo sapiens

text: Female

text: Placenta

text: Placenta 3' EST

text: Plasmid

text: pGEM-T


text: Yosu National University

text: live

text: Generic

text: Miamiansis avidus cultured in CHSE-214 cells were used for RNA isolation. cDNAs were directionally colned with Eco RI on the 5` and XhoI on the 3` end. Packing: Gigapack III Gold Packing Extract (Stratragene), Insert size: 0.5-5.0 kb, Mass Excison: XL1-Blue-MRF` (Stratragene), Helper phage: ExAssist (Stratrsgene), Titered on SOLR cells (Stratrsgene)

text: Miamiensis avidus

text: Eco R1

text: XhoI

characteristic: strain

text: Miamiensis avidus YS1 cDNA

text: phage

text: Uni-ZAP XR


text: Universite degli Studi di Torino

text: live

text: Generic

text: cDNA library from 100 germinated spores of Gigaspora margarita BEG34. The cDNA library was constructed using the SMART cDNA synthesis kit (Clontech, Palo Alto, CA, USA) according to the manufacturer's instructions. The resulting cDNA was packed into lambda phages using the Gigapack III Gold packaging kit (Stratagene, La Jolla, CA). The pTriplEx2 phagemid clones in E. coli were obtained by using the mass in vivo excision protocol according to the manufacturer's instructions (Clontech).

characteristic: isolate

text: Gigaspora margarita

text: germinated spores

characteristic: tissue-type

text: Gigaspora margarita germinated spores Library

text: Phagemid


text: DOE Joint Genome Institite

characteristic: INSDC first public

text: live

text: Generic

characteristic: NCBI submission model

text: The library was prepared from total RNA using the Creator SMART cDNA Library Construction Kit with the LD-PCR method to amplify the cDNA. Amplified cDNA was digested with SfiI, size selected for >400bp, and ligated into the pDNR-LIB vector.

text: Freshly spawned eggs

characteristic: dev-stage

text: ElectroMAX DH10B

text: Acropora palmata

text: SfiI

text: SfiI

text: AOKF Acropora palmata spawned eggs

text: pDNR-LIB


text: DOE Joint Genome Institite

text: live

text: Generic

characteristic: NCBI submission package

text: The library was prepared from total RNA using the Creator SMART cDNA Library Construction Kit with the LD-PCR method to amplify the cDNA. Amplified cDNA was digested with SfiI, size selected for >1000bp, and ligated into the pDNR-LIB vector.

characteristic: description

text: Freshly spawned eggs

text: ElectroMAX DH10B

text: Acropora palmata

text: SfiI

text: SfiI

text: AOKG Acropora palmata spawned eggs

text: pDNR-LIB


text: DOE Joint Genome Institite

text: live

text: Generic

text: Eggs

text: ElectroMAX DH10B

characteristic: lab-host

text: Orbicella faveolata

characteristic: organism

text: SfiI

text: SfiI

text: AOSB Montastraea faveolata spawned eggs

text: pDNR-LIB


text: DOE Joint Genome Institite

characteristic: INSDC last update

text: live

text: Generic

text: 60 hour post fertilization

text: ElectroMAX DH10B

text: Orbicella faveolata

text: SfiI

text: SfiI

text: AOSC Montastraea faveolata 60 hours post fertilization

characteristic: title

text: pDNR-LIB


characteristic: External Id

text: DOE Joint Genome Institite

text: live

text: Generic

text: The library was prepared from total RNA using the Creator SMART cDNA Library Construction Kit with the LD-PCR method to amplify the cDNA. Amplified cDNA was digested with SfiI, size selected for >400bp, and ligated into the pDNR-LIB vector. WARNING: this library contains a small percentage of cDNAs derived from the coral's symbiont, Symbiodinium sp.

text: Adult colony

text: ElectroMAX DH10B

text: Orbicella faveolata

text: SfiI

text: SfiI

text: AOSF Montastraea faveolata adult colony

text: pDNR-LIB

characteristic: vector


text: DOE Joint Genome Institute

text: live

characteristic: INSDC status

text: Generic

text: The library was made from dT primed cDNA and cloned into vector pDNR-Lib using the SMART system (BD Biosciences). To generate the first strand, poly A RNA were primed with an oligo dT primer (5'- ATTCTAGAGGCCGAGGCGGCCGACATG(T)30 VN -3', where V = A, G, or C). The second strand was synthesized by PCR using primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' followed by digestion of the SfiI sites. cDNA was size selected using 1% agarose gel electrophoresis (L ~0.5-1.2k, M ~1.2-2.5k, H ~>2.5k) then ligated into pDNR-Lib. The work was done at DOE Joint Genome Institute.

text: Adult

text: Pimephales promelas

text: Whole

text: AIZT Pimephales promelas Whole (L)

text: pDNR-LIB


text: Sri Krishnadevaraya University

text: live

text: Generic

characteristic: cultivar

text: Seeds were surface sterilized with 0.1% (w/v) sodium hypo chlorite solution for 5 min, thoroughly rinsed with distilled water and germinated in plastic pots contained soil and sand (2:1) mixture allowed to grow for nineteen days. The pots were maintained in the departmental botanical garden under natural photoperiod (10-12 hrs, 28 (4oC). Seedlings were maintained at 100% field capacity by watering daily. Nineteen day-old plants were subjected to moisture stress by withholding water. Leaf samples collected from unstressed (100% FC) and stressed (once field capacity reached 25%) plants, flash frozen and stored at -80oC. The frozen samples were used for RNA extraction (LiCl2 method). Poly(A) mRNA was isolated using streptavidin paramagnetic particles (SPMPs; Roche, Germany). First-strand cDNA synthesis was initiated from the enriched mRNA of driver (unstressed) using biotin labeled poly dT and Superscript RNaseH- reverse transcriptase (Gibco-BRL, USA). Subtractive hybridization was performed between biotin labeled driver ss cDNA (unstressed) and tester (stressed) mRNA at 65oC for 1 hour. The tester-driver hybrids and excess of the driver were separated from the unhybridized tester mRNA using streptavidin paramagnetic particles (SPMPs; Roche, Germany). Tester mRNAs that did not hybridize with the driver were used for cDNA synthesis. A primary cDNA library was produced using a lambda ZAP XR cDNA Synthesis Kit (Stratagene). Randomly selected clones amplified using M13F and M13R-primers, PCR product was purified using PCR Purification system (Stratagene) and sequenced from the 3' end on an MegaBACE 1000 DNA analysis system (Amersham Pharmacia, Uppsala, Sweden). Sequences are processed using the HarvEST pipeline (http://harvest.ucr.edu) to remove vector and cloning oligo sequences and various contaminants, and to trim to a high quality region. Sequences that retained at least 100 bases were deposited to GenBank.

text: 21 day-old seedlings

text: E.coli

text: Macrotyloma uniflorum

text: EcoRI

text: XhoI

text: Leaf

text: Horsegram (VZM-1) drought stressed subtracted cDNA

text: Phagemid pBluescript SK(-)

text: Lambda Uni-ZAP XR


text: Washington University School of Medicine

characteristic: INSDC center name

text: live

text: Generic

text: GoldRush

text: Total RNA was extracted from freeze dried leaf tissue, using the method described by Wang and Vodkin (Plant Molecular Biology Reporter 12:132-145, 1994). Poly(A)+ mRNA was isolated from total RNA using the PolyATtract mRNA Isolation system III (Promega). The library was prepared using the Stratagene pBluescript II XR cDNA library construction kit. Complementary DNA was synthesized from mRNA using a Poly (dT) sequence with a XhoI restriction site. EcoRI adapters were ligated to the blunt ended cDNA fragments followed by restriction with XhoI. The cDNA insert is protected from XhoI digestion via methylation during first strand cDNA synthesis. The cDNA fragments were directionally cloned into the EcoRI-XhoI restriction site of the pBluescript vector. The ligated cDNA fragments were transformed into E. coli ElectroMax DH10B host cells. Transformation efficiency: 1.0E+04 Average insert Size by PCR: 1290 bp

text: DH10B ampicillin resistant

text: Malus domestica

text: XhoI

text: EcoRI

text: Leaves challenged with apple scab (Venturia inaequalis)

text: Phagemid (ampicillin resistant)

text: pBluescript II SK (+)


text: live

text: Generic

text: GoldRush

text: Total RNA was extracted from freeze dried leaf tissue, using the method described by Wang and Vodkin (Plant Molecular Biology Reporter 12:132-145, 1994). Poly(A)+ mRNA was isolated from total RNA using the PolyATtract mRNA Isolation system III (Promega). The library was prepared using the Stratagene pBluescript II XR cDNA library construction kit. Complementary DNA was synthesized from mRNA using a Poly (dT) sequence with a XhoI restriction site. EcoRI adapters were ligated to the blunt ended cDNA fragments followed by restriction with XhoI. The cDNA insert is protected from XhoI digestion via methylation during first strand cDNA synthesis. The cDNA fragments were directionally cloned into the EcoRI-XhoI restriction site of the pBluescript vector. The ligated cDNA fragments were transformed into E. coli ElectroMax DH10B host cells. Transformation efficiency: 4.0E+04 Average insert Size by PCR: 1320 bp

text: DH10B ampicillin resistant

text: Malus domestica

text: XhoI

text: EcoRI

text: Leaves challenged with oblique banded leaf roller (Choristoneura rosaceana)

text: Phagemid (ampicillin resistant)

text: pBluescript II SK (+)


text: live

text: Generic

text: red delicious

text: Total RNA was extracted from freeze dried leaf tissue, using the method described by Wang and Vodkin (Plant Molecular Biology Reporter 12:132-145, 1994). Poly(A)+ mRNA was isolated from total RNA using the PolyATtract mRNA Isolation system III (Promega). The library was prepared using the Stratagene pBluescript II XR cDNA library construction kit. Complementary DNA was synthesized from mRNA using a Poly (dT) sequence with a XhoI restriction site. EcoRI adapters were ligated to the blunt ended cDNA fragments followed by restriction with XhoI. The cDNA insert is protected from XhoI digestion via methylation during first strand cDNA synthesis. The cDNA fragments were directionally cloned into the EcoRI-XhoI restriction site of the pBluescript vector. The ligated cDNA fragments were transformed into E. coli ElectroMax DH10B host cells. Transformation efficiency: 1.30E+05 Average insert Size by PCR: 1420 bp

text: DH10B ampicillin resistant

text: Malus domestica

text: XhoI

text: EcoRI

text: Leaves challenged with fire blight (Erwinia amylovora)

text: Phagemid (ampicillin resistant)

text: pBluescript II SK (+)


text: live

text: Generic

text: Total RNA was extracted separately from whole root using the 'pine tree' method. Poly(A)+mRNA was isolated twice from total RNA from each stage using the Oligotex Direct mRNA kit (Qiagen). mRNA was reverse transcribed into double stranded cDNA using a modified oligo18(dT) primer. Double stranded cDNAs were size selected (more than 450 bp), adaptored with EcoRI adapters at both ends and then digested with NotI. The cDNAs were then directionally cloned into EcoR1-NotI digested pBS II SK(+) phagemid vector(Stratagene). The total number of white colony forming units (cfu) was 1.5 x 10^6 cfu cfu (colony forming units). The background of empty clones was less than 6%. Inserts ranged from 0.5kb to 4 kb, as determined by PCR.

text: young root

text: DH10B ampicillin resistant

text: Malus domestica

text: Not I

text: EcoRI

text: Root

text: Phagemid (ampicillin resistant)

text: pBluescript II SK (+)


text: Centro de Investigacion y de Estudios Avanzados del IPN

text: live

text: Generic

text: A Suppression Subtractive Hybridization (SSH) experiment was performed with the PCR-Select cDNA substraction kit (Clontech, USA). The driver sample consisted of pooled Poly(A)-RNA isolated from four roots growing on soil without any added petroleum, the tester sample consisted of pooled Poly(A)-RNA isolated from four roots growing on soil containing a petroleum concentration of 10%, which was in the estimated CE50 confidence range. Both samples were from 30 day old plants.

text: 30 day old stolons

text: E. coli TOP 10'F

text: Root

characteristic: organ

text: Cynodon dactylon

text: RsaI

text: RsaI

text: Fernandez 630

characteristic: strain

text: Cynodon dactylon roots growing under petroleum hydrocarbon stress

text: pDrive


text: live

text: Generic

text: Geneva 3041 x Malus siversii apple rootstock. Total RNA was extracted separately from whole root using the 'pine tree' method. Poly(A)+mRNA was isolated twice from total RNA from each stage using the Oligotex Direct mRNA kit (Qiagen). mRNA was reverse transcribed into double stranded cDNA using a modified oligo18(dT) primer. Double stranded cDNAs were size selected (more than 450 bp), adaptored with EcoRI adapters at both ends and then digested with NotI. The cDNAs were then directionally cloned into EcoR1-NotI digested pBS II SK(+) phagemid vector(Stratagene). The total number of white colony forming units (cfu) was 1.5 x 10^6 cfu cfu (colony forming units). The background of empty clones was less than 6%. Inserts ranged from 0.5kb to 4 kb, as determined by PCR.

text: phytophtora challenged young root

text: DH10B ampicillin resistant

text: Malus domestica x Malus sieversii

text: Not I

text: EcoRI

text: Geneva 3041 x Malus sieversii

text: Root

characteristic: tissue-type

text: Phagemid (ampicillin resistant)

text: pBluescript II SK (+)


text: College of Animal Husbandry, Konkuk Univ.

characteristic: INSDC first public

text: live

text: Generic

text: Sus scrofa

text: Korean native pig full-length enriched library


text: National Institute of Agrobiological Sciences

text: live

text: Generic

characteristic: NCBI submission model

text: adult

text: Sus scrofa

text: alveolar macrophage

text: full-length enriched swine cDNA library, adult alveolar macrophage


text: live

text: Generic

text: adult

characteristic: dev-stage

text: Sus scrofa

text: intestine

text: full-length enriched swine cDNA library, adult intestine


text: Volcani Center

text: live

text: Generic

characteristic: NCBI submission package

text: 20 days post infection

text: Heterodera avenae

text: Wheat infected by cyst nematode

text: Plasmid

text: pDesT22 (invitrogen)


text: National Cancer Institute / NIH

text: live

text: Generic

text: 1st strand cDNA was primed with an oligo(dT) primer 5'-GCGGCTGAAGACGGCCTATGTGGCCTTTTTTTTTTTTTTTTT-3'; double-stranded cDNA was ligated to a DraIII adaptor 5'-GGCCUACUGG-3', digested and directionally cloned into distinct DraIII sites of the pME18S-FL3. Library was size selected for >1.0 kb, with a average insert size of ~1.2 kb and is not amplified. Library constructed by Yutaka Suzuki (University of Tokyo Institute of Medical Science). Custom primers recommended for sequencing: 5' end primer 5'-GGATGTTGCCTTTACTTCTA-3' and 3' end primer 5'-CGACCTGCAGCTCGAGCACA-3'. Reference for library construction: Methods Mol Bio 221:73-91. Note: this is a (http://zgc.nci.nih.gov/) Zebrafish Gene Collection library.

characteristic: description

text: DH10B TonA

text: skin

text: Danio rerio

text: DraIII

text: DraIII

text: 40 pooled samples

text: phagemid


text: National Cancer Institute / NIH

text: live

text: Generic

text: cDNA was primed using oligo-dT primer: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3' and cloned into the EcoRV/NotI sites of pExpress-1. Size-selection 1.2kb resulted in an average insert size of 1.8kb. This is a primary library (normalized library is NICHD_XGC_FabN) and was constructed by Express Genomics (Frederick, MD). Note: this is a (http://xgc.nci.nih.gov/) Xenopus Gene Collection library.

text: DH10B TonA

characteristic: lab-host

text: fat body

text: Xenopus laevis

text: EcoRV

text: NotI

text: plasmid


text: National Cancer Institute / NIH

text: live

text: Generic

text: cDNA was primed using oligo-dT primer: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3' and cloned into the EcoRV/NotI sites of pExpress-1. Size-selection 1.2kb resulted in an average insert size of 1.5kb, and Cot value of 7. This is a normalized library (primary library is NICHD_XGC_FaB) and was constructed by Express Genomics (Frederick, MD). Note: this is a ( http://xgc.nci.nih.gov/) Xenopus Gene Collection library.

text: DH10B TonA

text: fat body

text: Xenopus laevis

characteristic: organism

text: EcoRV

text: NotI

text: plasmid


text: Sri Krishnadevaraya University

characteristic: INSDC last update

text: live

text: Generic

text: Seeds were surface sterilized with 0.1% (w/v) sodium hypo chlorite solution for 5 min, thoroughly rinsed with distilled water and germinated in plastic pots contained soil and sand (2:1) mixture allowed to grow for nineteen days. The pots were maintained in the departmental botanic garden under natural photoperiod (10-12 hrs, 28 ( 4oC). Seedlings were maintained at 100% field capacity by watering daily. Nineteen day-old plants were subjected to moisture stress by withholding water. Leaf samples collected from unstressed (100% FC) and stressed (once field capacity reached 25%) plants, flash frozen and stored at -80oC. The frozen samples were used for RNA extraction (LiCl2 method). Poly(A) mRNA was isolated using streptavidin paramagnetic particles (SPMPs; Roche, Germany). First-strand cDNA synthesis was initiated from the enriched mRNA of driver (unstressed) using biotin labeled poly dT and Superscript RNaseH- reverse transcriptase (Gibco-BRL, USA). Subtractive hybridization was performed between biotin labeled driver sscDNA (unstressed) and tester (stressed) mRNA at 65oC for 1 hour. The tester-driver hybrids and excess of the driver were separated from the unhybridized tester mRNA using streptavidin paramagnetic particles (SPMPs; Roche, Germany). Tester mRNAs that did not hybridize with the driver were used for cDNA synthesis (Stratagene). A primary cDNA library was produced using a lambda ZAP XR cDNA Synthesis Kit (Stratagene). Randomly selected clones amplified using M13F and M13R-primers, PCR product was purified using PCR Purification system (Stratagene) and sequenced from the 3' end on an MegaBACE 1000 DNA analysis system (Amersham Pharmacia, Uppsala, Sweden). Sequences are processed using the HarvEST pipeline (http://harvest.ucr.edu) to remove vector and cloning oligo sequences and various contaminants, and to trim to a high quality region. Sequences that retained at least 100 bases were submitted to GenBank.

text: 21 day-old seedlings

text: E.coli

text: Arachis hypogaea

text: EcoRI

text: XhoI

text: Leaf

text: Groundnut (K-134) drought stressed subtracted cDNA library

text: Phagemid

text: Lambda Uni-ZAP XR


text: Institute of Aquaculture

text: live

text: Generic

text: Larval

text: XLOLR

text: Dicentrarchus labrax

text: Dicentrarchus labrax larval cDNA

characteristic: title

text: phagemid

text: pBK-CMV


characteristic: External Id

text: Kansas State University

text: live

text: Generic

text: Chisholm

text: Triticum aestivum

text: Roots

text: Wheat cv. Chisholm Aluminum SSH Library


text: Roslin Institute

text: live

text: Generic

text: Normalised cDNA library.

text: Sus scrofa

text: NotI

text: EcoRI

text: Large White

text: spleen

text: pBluescript II KS

characteristic: vector


text: Wellcome Trust Sanger Institute

text: live

characteristic: INSDC status

text: Generic

text: country: Puerto Rico

text: lung schistosomulum

text: Schistosoma mansoni

text: Schistosoma mansoni lung schistosomulum


text: Roslin Institite

text: live

text: Generic

text: Gallus gallus

text: EcoRI

text: NotI

text: Lohman Brown layer/Ross 308 broiler cross

text: thymus

text: Chicken immune 6 - CSEQRBN34

text: pBluescript II kS(+)


text: Roslin Institite

text: live

text: Generic

text: Gallus gallus

text: EcoRI

text: NotI

text: Bursa, spleen, Peyers patch (pooled)

text: Chicken immune 2 - CSEQRBN29

text: pBluescript II kS(+)


text: Roslin Institite

text: live

text: Generic

text: Gallus gallus

text: EcoRI

text: NotI

text: thymus

text: Chicken immune 4 - CSEQRBL09

text: pBluescript II kS(+)


text: Roslin Institite

text: live

text: Generic

text: Gallus gallus

text: EcoRI

text: NotI

text: Bursa, spleen, Peyers patch (pooled)

text: Chicken immune 3 - CSEQRBN33

text: pBluescript II kS(+)


text: Roslin Institite

text: live

text: Generic

text: Gallus gallus

text: thymus

text: Chicken immune 5 - CSEQRBN30


text: University of Leicester

text: live

text: Generic

text: country: China:Yunnan, Long Pu; isolation_source: algal mat; environmental_sample

text: uncultured organism

text: algal mat environmental sample

text: Lambda TriplEx2


text: University of Leicester

text: live

text: Generic

text: country: United Kingdom:Leicestershire ; isolation_source: sewage treatment plant ; environmental_sample

text: uncultured organism

text: sewage treatment plant environmental sample

text: Lambda TriplEx2


text: Virology UMR GDPP INRA/UB2 IBVM, INRA/AGRO.M

text: live

text: Generic

text: Goldrich

characteristic: cultivar

text: Prunus armeniaca

text: Prunus armeniaca cv. Goldrich


text: NEAU

characteristic: INSDC center name

text: live

text: Generic

text: Glycine soja

text: Salt-treated full-length cDNA library of wild soybean


text: University of Veterinary and Pharmaceutical Sciences Brno

text: live

text: Generic

text: macrophage

characteristic: cell type

text: Equus caballus

text: Equus caballus macrophage library


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from dT primed cDNA and cloned into vector pDNR-Lib using the SMART system (BD Biosciences). To generate the first strand, poly A RNA were primed with an oligo dT primer (5'- ATTCTAGAGGCCGAGGCGGCCGACATG(T)30 VN -3', where V = A, G, or C). The second strand was synthesized by PCR using primer 5'- AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG - 3' followed by digestion of the SfiI sites. cDNA was size selected using 1% agarose gel electrophoresis (L ~0.5-1.2k, M ~1.2-2.5k, H ~>2.5k) then ligated into pDNR-Lib. The work was done at DOE Joint Genome Institute.

text: Adult

text: Pimephales promelas

text: Whole

text: AIZU Pimephales promelas Whole (M)

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: Adult

text: Pimephales promelas

text: Whole

text: AIZW Pimephales promelas Whole (H)

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: Adult

text: Pimephales promelas

text: Whole

text: ANNK Pimephales promelas Whole (L)

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from dT primed cDNA and cloned into Invitrogen vector pDONR222. Poly A RNA were primed with a Biotin-attB2-Oligo(dT) primer (5'- GGCGGCCGCACAACTTTGTACAAGAAAGTTGGGT(T)19 -3') and the first strand synthesized using Superscript II (Invitrogen). The second strand was synthesized and an attB1 adaptor was ligated to the 5' end (5'- TCGTCGGGGACAACTTTGTACAAAAAAGTTGG -3' and 5'- CCAACTTTTTTGTACAAAGTTGTCCCC -3') . cDNA was size selected using 1% agarose gel electrophoresis (L ~0.5-1.2k, M ~1.2-2.5k, H ~>2.5k) and then inserted into the vector using site specific recombination (flanking attB sites on cDNA). The work was done at DOE Joint Genome Institute.

text: Adult

text: Pimephales promelas

text: Whole

text: ANNN Pimephales promelas Whole (L)


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from dT primed cDNA and cloned into Invitrogen vector pDONR222. Poly A RNA were primed with a Biotin-attB2-Oligo(dT) primer (5'- GGCGGCCGCACAACTTTGTACAAGAAAGTTGGGT(T)19 -3') and the first strand synthesized using Superscript II (Invitrogen). The second strand was synthesized and an attB1 adaptor was ligated to the 5' end (5'- TCGTCGGGGACAACTTTGTACAAAAAAGTTGG -3' and 5'- CCAACTTTTTTGTACAAAGTTGTCCCC -3') . The cDNA was size selected using 1% agarose gel electrophoresis. (L ~0.5-1.2k, M ~1.2-2.5k, H ~>2.5k) and then inserted into the vector using site specific recombination (flanking attB sites on cDNA). The work was done at DOE Joint Genome Institute.

text: Adult

text: Pimephales promelas

text: Whole

text: ANNO Pimephales promelas Whole (M)


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from dT primed cDNA and cloned into Invitrogen vector pCMVsport6. Poly A RNA were primed with an oligo dT primer (5'- GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT -3') ligated to a SalI adapter (5'- TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected using 1.1% agarose gel electrophoresis (L ~0.5-1.2k, M ~1.2-2.5k, H ~>2.5k) then ligated into NotI and SalI digested pCMVsport6 vector. The work was done at DOE Joint Genome Institute.

text: Pimephales promelas

text: brain

text: CAAS Pimephales promelas brain 7-8 month adults, males and females pooled (H)


text: DOE Joint Genome Institute

text: live

text: Generic

text: Pimephales promelas

text: brain

text: CAAT Pimephales promelas brain 7-8 month adults, males and females pooled (M)


text: DOE Joint Genome Institute

text: live

text: Generic

text: Pimephales promelas

text: brain

text: CAAU Pimephales promelas brain 7-8 month adults, males and females pooled (L)


text: DOE Joint Genome Institute

text: live

text: Generic

text: Pimephales promelas

text: testis

text: CAAV Pimephales promelas testis 7-8 month adults, males and females pooled (H)


text: DOE Joint Genome Institute

text: live

text: Generic

text: Pimephales promelas

text: testis

text: CAAW Pimephales promelas testis 7-8 month adults, males and females pooled (M)


text: DOE Joint Genome Institute

text: live

text: Generic

text: Pimephales promelas

text: testis

text: CAAX Pimephales promelas testis 7-8 month adults, males and females pooled (L)


text: DOE Joint Genome Institute

text: live

text: Generic

text: Adult

text: Pimephales promelas

text: kidney

text: CABR Pimephales promelas kidney 7-8 month adults, males and females pooled (H)


text: DOE Joint Genome Institute

text: live

text: Generic

text: Adult

text: Pimephales promelas

text: kidney

text: CABS Pimephales promelas kidney 7-8 month adults, males and females pooled (M)


text: DOE Joint Genome Institute

text: live

text: Generic

text: Adult

text: Pimephales promelas

text: kidney

text: CABT Pimephales promelas kidney 7-8 month adults, males and females pooled (L)


text: DOE Joint Genome Institute

text: live

text: Generic

text: Adult

text: Pimephales promelas

text: ovary

text: CABU Pimephales promelas ovary 7-8 month adults, females pooled (H)


text: DOE Joint Genome Institute

text: live

text: Generic

text: Adult

text: Pimephales promelas

text: ovary

text: CABW Pimephales promelas ovary 7-8 month adults, females pooled (M)


text: DOE Joint Genome Institute

text: live

text: Generic

text: Adult

text: Pimephales promelas

text: ovary

text: CABX Pimephales promelas ovary 7-8 month adults, females pooled (L)


text: DOE Joint Genome Institute

text: live

text: Generic

text: 2-14 day Post-Hatch Fry

text: Pimephales promelas

text: Whole

text: CABY Pimephales promelas 2-14 day post-hatch fry (H)


text: DOE Joint Genome Institute

text: live

text: Generic

text: 2-14 day Post-Hatch Fry

text: Pimephales promelas

text: Whole

text: CABZ Pimephales promelas 2-14 day post-hatch fry (M)


text: DOE Joint Genome Institute

text: live

text: Generic

text: 2-14 day Post-Hatch Fry

text: Pimephales promelas

text: Whole

text: CACA Pimephales promelas 2-14 day post-hatch fry (L)


text: DOE Joint Genome Institute

text: live

text: Generic

text: Adult

text: Pimephales promelas

text: liver

text: CACC Pimephales promelas liver 7-8 month adults, males and females pooled (M)


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from dT primed cDNA and cloned into Invitrogen vector pCMVsport6. Poly A RNA were primed with an oligo dT primer (5'- GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT -3') ligated to a SalI adapter (5'- TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected using 1.1% agarose gel electrophoresis (L ~0.5-1.2k, M ~1.2-2.5k, H ~>2.5k) then ligated into NotI and SalI digested pCMVsport6 vector. The work was done at DOE Joint Genome Institute.

text: Adult

text: Pimephales promelas

text: liver

text: CACD Pimephales promelas liver 7-8 month adults, males and females pooled (L)


text: National Institute of Genetics, Center for Information Biology and DDBJ

text: live

text: Generic

text: Homo sapiens

text: eye

text: Homo sapiens eye


text: Universite des Sciences et Technologies de Lille

text: live

text: Generic

text: Day 4 of somatic embryogenesis induction

text: Leaf

characteristic: organ

text: Cichorium intybus

text: Non embryogenic SSH library

text: Plasmid

text: PGEM


text: live

text: Generic

text: Leaf

text: Cichorium intybus

text: Embryogenic SSH library

text: Plasmid

text: PGEM


text: Department of Life Science, National Taiwan Normal University

text: live

text: Generic

text: Wolfiporia cocos

characteristic: strain

text: mycelium

characteristic: tissue-type

text: Wolfiporia cocos 1-week-old cDNA library

text: plasmid

text: pGEM-T


text: University of California

characteristic: INSDC first public

text: live

text: Generic

characteristic: NCBI submission model

text: Swingle

text: Twelve seedlings were transplanted from cones to 4 inch pots of sand mix, then grown in greenhouses (Darsow, Becker lab). Citrus nematodes (Tylenchulus semipenitrans) were extracted from contaminated soil from the UC Riverside Citrus Research Center then suspended in water and added to pots (Darsow). Growing roots were collected by clipping them away from the old root ball 24 hr and 4 days after inoculation (Federici). Total RNA was extracted using Concert Plant RNA Reagent (Invitrogen)followed by RNeasy (Qiagen), an equal amount of RNA was pooled from each of the two collection times, poly(A) RNA was purified using Oligotex (Qiagen), a cDNA library was made using a cDNA synthesis kit (Stratagene), then 0.45 million primary lambda cDNA clones were in vivo excised to give a population of pBluescript SK(-) phagemids (Fenton, Close lab). Phagemids were plated, plasmid DNA purified, cDNA clones archived, and DNA sequences determined bi-directionally using an ABI3730 at the University of California Riverside Institute of Integrative Genome Biology Genomics Core Instrumentation Facility, (Choi, Kingan). Chromatogram files were downloaded by FTP by Close, then processed by Wanamaker (Close lab) using the HarvEST pipeline (http://harvest.ucr.edu) to remove vector and cloning oligo sequences and various contaminants, and to trim to a high quality region. Sequences that retained a phred 17 region of at least 100 bases were assembled, then chimeras were removed following manual inspection of assemblies (Close, Roose, Wanamaker). Sequences that survived all removal steps were submitted to GenBank.

text: seedling

text: E. coli TJC121

text: Citrus x paradisi x Citrus trifoliata

text: EcoRI

text: XhoI

text: root

text: Swingle citrumelo nematode-challenged root cDNA library - UCRCP01-2

text: Phagemid pBluescript SK(-)

text: Lambda Uni-ZAP XR, excised phagemid


text: University of California

text: live

text: Generic

characteristic: NCBI submission package

text: Ruby

text: Two trees with open-pollinated flowers in a mixed planting at UC Riverside Agricultural Operations were the source of tissue. Flower buds were collected by Federici (Roose lab) from January -March 2003 in four groups: 1) no white petals, 2) white petals visible, bud less than 0.5 cm, 3) bud more than 0.5 cm but not open, 4) newly opened flowers. Tissues were snap frozen and then stored at -80C until further processing. Fenton (Close lab) purified RNA by the phenol method described in J. Japanese Soc. Hort. Sci. 1996. 64 (4): 809-814, purified poly(A) mRNA using a PolyATtrack mRNA Isolation System IV (Promega), produced a primary cDNA library using a lambda ZAP XR cDNA Synthesis Kit (Stratagene), then mass-excised one million pfu from the primary library to produce a phagemid population. Phagemids were plated, plasmid DNA purified, cDNA clones archived, and DNA sequences determined bi-directionally at the Arizona Genomics Institute (Kim, Kudrna, Stum, Yost, Wing). Leftover plasmid DNA was re-sequenced on the 3' end using an ABI3730 at the UC Riverside Genomics Core Facility (Choi, Kingan). Chromatogram files were downloaded by Close, then processed by Wanamaker (Close lab) using the HarvEST pipeline (http://harvest.ucr.edu) to remove vector and cloning oligo sequences and various contaminants, and to trim to a high quality region. Sequences that retained a phred 17 region of at least 100 bases were deposited to GenBank.

text: 20 year old trees

characteristic: dev-stage

text: E. coli TJC121

text: Citrus sinensis

text: EcoRI

text: XhoI

text: Flower

text: Ruby Orange Developing Flower cDNA Library UCRCS04-2

text: Phagemid pBluescript SK(-)

text: Lambda Uni-ZAP XR, excised phagemid