text: Warsaw Agricultural University, Faculty of Horticulture and Landscape Architecture

text: live

text: Generic

text: The cDNA from 1-2 mm male floral buds was digested with MboI restriction enzyme and amplified. To obtain this 'subtractome' library was obtained after subtraction using cDNA-DSC method between near isogenic lines: B10 (monoecious ffMMGyGy) - used as a tester and 2gg (gynoecious -ffMMgygy)- used as a driver

text: 1-2 mm

text: 1-2 mm flower bud - male

text: Cucumis sativus

text: male (monoecious)

characteristic: strain

text: male flower bud cucumber cDNA-DSC B10/2gg


status: PUBLIC

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: The cDNA from 1-2 mm floral buds was digested with MboI restriction enzyme and amplified. To obtain this 'subtractome' library was obtained after subtraction using cDNA-DSC method between near isogenic lines: Gy3 (gynoecious FFMMGyGy)- used as a tester and HGY3 (hermaphrodite -FFmmGyGy) - used as a driver

characteristic: description

text: 1-2 mm

tag: attribute

characteristic: dev-stage

text: 1-2 mm flower bud - female

characteristic: organ

text: Cucumis sativus

characteristic: organism

text: female(gynoecious)

characteristic: sex

text: female flower bud cucumber cDNA-DSC Gy3/Hgy3

characteristic: title


text: The Institute for Genomic Research

text: live

text: Generic

text: 7-56 mother tree, open-pollinated tree from, Lyons, Georgia, USA

characteristic: cultivar

text: Library enriched in transcripts present in early phase of loblolly pine embryo maturation. A subtractive library was made using the protocols of Clontech (Palo Alto,CA). RNA from loblolly pine embryos in which cotyledons had just emerged (Stage 9.1, see Ciavatta VT, Morillon R, Pullman GS, Chrispeels M, Cairney J. 2001. An aquaglyceroporin is abundantly expressed early in the development of the suspensor and the embryo proper of loblolly pine (Pinus taeda L.). Plant Physiol. 127: 1556-1567 [http://www.plantphysiol.org/cgi/content/full/127/4/1556]) was subtracted from RNA from pine embryos collected two weeks after cotyledon emergence (Stage 9.3).

characteristic: lab-host

text: Zygotic Embryos

text: Pinus taeda

text: EcoRI

text: SpeI

text: Whole embryos excised from megagametophytes

characteristic: tissue-type

text: Subtracted pine embryo library, Lib_B

text: plasmid

text: pGEM-T Easy

characteristic: vector


characteristic: External Id

text: The Institute for Genomic Research

text: live

text: Generic

text: Library enriched in transcripts present in late phase of loblolly pine embryo maturation. A subtractive library was made using the protocols of Clontech (Palo Alto,CA). RNA from loblolly pine embryos eight weeks after cotyledon emergence (Stage 9.8, see Ciavatta VT, Morillon R, Pullman GS, Chrispeels M, Cairney J. 2001. An aquaglyceroporin is abundantly expressed early in the development of the suspensor and the embryo proper of loblolly pine (Pinus taeda L.). Plant Physiol. 127: 1556-1567 [http://www.plantphysiol.org/cgi/content/full/127/4/1556]) was subtracted from RNA from pine embryos collected nine weeks after cotyledon emergence (Stage 9.9).

text: Zygotic Embryos

text: Pinus taeda

text: EcoRI

text: SpeI

text: Subtracted pine embryo library, Lib_C

text: plasmid

text: pGEM-T Easy


text: Dept of AFNS, University of Alberta

characteristic: INSDC center name

text: live

text: Generic

text: epithelial

characteristic: cell type

text: young adult

text: Echerichia coli DH10B

text: gastrointestinal tract

text: Bos taurus

text: BamH I

text: Xho I

text: female

text: peyer's patch

text: GC_BGC-01 Library

text: Phage-resistant

text: pBlueScript II SK(+)


text: USDA-ARS

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Stems were harvested from field grown plants

text: E. coli

text: 10 week old stems

text: Gossypium hirsutum

text: PCR amplified cotton stem cDNA

text: Topo TA


text: Department of Marine Biotechnology, Cheju National University

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: digestive gland and gonad

text: Haliotis discus discus

text: Eco RI

text: Xho I

text: both male and female

text: Abalone digestive gland and gonad cDNA library

text: Phage

text: pBluescript SK-1


text: University of St Andrews

text: live

text: Generic

characteristic: NCBI submission package

text: Library was prepared by Helene Pagander and Hannah Chubb and sequenced at the John Innes Centre, Norwich, UK

characteristic: description

text: Adductor muscle

text: Pecten maximus

text: Helene and Hannah


text: ARO, The Volcani Center

text: live

text: Generic

text: Orah

text: Citrus temple hort. ex Tanaka (Temple tangor) X Citrus reticulata Blanco cultigroup Tangerine cv. 'Dancy Tangerine' ; Poly-A mRNA was isolated from total RNA, and cDNA synthesis and library construction was performed using SMART cDNA Library Construction Kit (Clontech laboratories, Inc, Catalog # K1051-1) according to the manufacturer's instructions (Protocol # PT3000-1, Version # PR15738).

text: 7-10 DBFB

characteristic: dev-stage

text: E. Coli BM25.8

text: Ovaries of closed flowers

characteristic: organ

text: Citrus reticulata x Citrus temple

text: SfiIA

text: SfiIB

text: Phagemid


text: ARO, The Volcani Center

text: live

text: Generic

text: Or

text: Poly-A mRNA was isolated from total RNA of Or (irradiated Citrus temple hort. ex Tanaka (Temple tangor) X Citrus reticulata Blanco cultigroup Tangerine cv. 'Dancy Tangerine'). cDNA synthesis and library construction was performed using SMART cDNA Library Construction Kit (Clontech laboratories, Inc, Catalog # K1051-1) according to the manufacturer's instructions (Protocol # PT3000-1, Version # PR15738).

text: 7-10 DAFB

text: E. Coli BM25.8

text: Ovaries of open flowers

text: Citrus reticulata x Citrus temple

characteristic: organism

text: SfiIA

text: SfiIB

text: Phagemid


text: USDA/ARS

text: live

text: Generic

text: paymaster HS26

text: 3 week old cold stressed seedling

text: E. coli

text: 3 week old seedlings

text: Gossypium hirsutum

text: 3 week old cold stressed seedling

characteristic: title

text: BlueScript


text: USDA/ARS

text: live

text: Generic

text: Coker 312

characteristic: cultivar

text: 15-20 DAA bolls from drought stressed plants

text: E. coli

text: Gossypium hirsutum

text: BlueScript


text: USDA/ARS

text: live

text: Generic

text: Coker 312

text: 15-20 DAA bolls from irrigated plants

text: E. coli

characteristic: lab-host

text: 15-20 DAA bolls from irrigated plants

text: Gossypium hirsutum

text: 15-20 DAA bolls from irrigated plants

text: BlueScript


text: USDA/ARS

text: live

text: Generic

text: Coker 312

text: 8-10 DAA cotton bolls from drought stressed plants

text: E. coli

text: Gossypium hirsutum

text: BlueScript


text: Oklahoma State University

text: live

text: Generic

text: (AcB4 x AcBIn) x Acb7

text: Equal quantities of RNA from both stages were combined prior to mRNA isolation. In the SSH cDNA subtraction procedure, the reference cDNA was from non-inoculated leaves.

text: E. coli

text: leaf 8, 14 hrs post inoculation

text: Gossypium hirsutum

text: Leaf cDNA 8,14 hrs post inoculation with Xanthomonas campestris pv. malvacearum 3631

text: pCRScriptAmpSK


text: Oklahoma State University

text: live

text: Generic

text: (AcB4 x AcBIn) x Acb7

text: E. coli

text: leaf 20, 30 hrs post inoculation

text: Gossypium hirsutum

text: Leaf cDNA 20,30 hrs post inoculation with Xanthomonas campestris pv. malvacearum 3631

text: pCRScriptAmpSK(+) or pCR2.1-TOPO


text: Oklahoma State University

text: live

text: Generic

text: (AcB4 x AcBIn) x Acb7

text: E. coli

text: leaf 45, 60 hrs post inoculation

text: Gossypium hirsutum

text: Leaf cDNA 45, 60 hrs post inoculation with Xanthomonas campestris pv. malvacearum 3631

text: pCR4-TOPO or pCR2.1-TOPO


text: Penn State Erie-The Behrend College

text: live

text: Generic

text: Russet Burbank

text: Dormant tubers

text: Solanum tuberosum

text: Excised Meristems

characteristic: tissue-type

text: Potato dormant meristem library

text: Lambda Bluescript SK-


text: Oklahoma State University

text: live

text: Generic

text: cDNA suppression subtraction library from oropharyngeal tonsils and mandibular lymph nodes of diseased and healthy European wild boar naturally exposed to M. bovis. Total RNA was isolated using TriReagent (Sigma, St. Louis, MO, USA) according to manufacturer's instructions. After diagnosis of diseased and healthy individuals by pathology, culture and spoligotyping of mycobacteria, pools of 20 ug RNA were made for healthy (N=2; 10 ug RNA/each) and diseased (N=4; 5 ug RNA/each) animals. SSH was performed at Evrogen JCS (Moscow, Russia) as previously described (Diachenko et al., 1996; 1999). Tester and driver RNAs were subtracted in both directions to construct 4 SSH libraries containing differentially expressed cDNAs in diseased (reverse-subtracted) and healthy (forward-subtracted) samples for oropharyngeal tonsils and mandibular lymph nodes. Approximately 100 clones from each library were randomly picked up and subjected to differential hybridization with subtracted and non-subtracted probes using the PCR-select differential screening kit (Clontech, Palo Alto, CA, USA), which resulted in >90% differentially expressed cDNAs.

text: Escherichia coli

text: Sus scrofa

text: Female

characteristic: sex

text: Plasmid

characteristic: vector


characteristic: External Id

text: University of Georgia

characteristic: INSDC center name

text: live

text: Generic

text: Cronartium quercuum f. sp. fusiforme cDNA common to both indirectly (from glass surface) and directly germinating basidiospores (from polystyrene surface).

text: germinating basidiospores

text: Cronartium quercuum f. sp. fusiforme

text: Suppression subtraction hybridization library with cDNA common to both directly and indirectly germinating basidiospores


text: University of Georgia

characteristic: INSDC first public

text: live

text: Generic

text: cDNA from Cronartium quercuum f. sp. fusiforme basidiospores germinating directly on polystyrene surfaces were hybridized to cDNA from Cronartium quercuum f. sp. fusiforme basidiospores germinating indirectly on glass surfaces in order to enrich for sequences expressed in directly germinating basidiospores.

text: germinating basidiospores

text: Cronartium quercuum f. sp. fusiforme

text: Directly germinating basidiospore suppression subtraction hybridization library


text: University of Georgia

characteristic: INSDC last update

text: live

text: Generic

text: cDNA from Cronartium quercuum f. sp. fusiforme indirectly germinating basidiospores (from glass surface) was hybridized to cDNA from Cronartium quercuum f. sp. fusiforme directly germinating basidiospores (from polystyrene surface) in order to enrich for sequences expressed in indirectly germinating basidiospores.

text: germinating basidiospores

text: Cronartium quercuum f. sp. fusiforme

text: Indirectly germinating basidiospore suppression subtraction hybridization library


text: Michigan State University

text: live

characteristic: INSDC status

text: Generic

text: cDNAs were derived from reverse transcription of mRNA isolated from complete inflorescences harvested at 9 AM and 3 PM from field grown bolting beets on July 6, 2004.

text: all

text: E. coli DH10b

text: inflorescence

text: Beta vulgaris

text: Sfi A

text: Sfi B

text: flower stalk, mature and developing seeds, flowers, floral meristems

text: Sugar beet field-harvested seed stalks

text: pDNA-LIB


text: National Institute of Allergy and Infectious Diseases

text: live

text: Generic

characteristic: NCBI submission model

text: library was constructed using long distance PCR (Smart cDNA kit) and Lambda TriplEx2 vector

text: adults 1-7 days after adult eclosion not blood fed

text: salivary glands

text: Stomoxys calcitrans

text: male and female

text: Adult salivary gland cDNA library XWJRABMK

text: Phage

text: Lambda TriplEx2


text: live

text: Generic

characteristic: NCBI submission package

text: unidirectional library was constructed using long distance PCR (Smart cDNA kit) and Lambda TriplEx2 vector

text: adults 3-4 days after attachment to a rabbit

text: salivary glands

text: Ixodes scapularis

text: female

text: Adult female salivary gland cDNA library ISA72

text: Phage

text: Lambda TriplEx2


text: live

text: Generic

characteristic: description

text: nymphs infected with Borrelia burgdorferi 48h after attachment to a rabbit

text: salivary glands

text: Ixodes scapularis

text: male and female

text: infected nymphal salivary gland cDNA library ISNINF

text: Phage

text: Lambda TriplEx2


text: live

text: Generic

text: nymphs 48 h after attachment to a rabbit

characteristic: dev-stage

text: salivary glands

text: Ixodes scapularis

text: male and female

text: nymphal salivary gland cDNA library ISNU

text: Phage

text: Lambda TriplEx2


text: live

text: Generic

text: adult unfed

text: salivary glands

characteristic: organ

text: Ixodes scapularis

text: female

text: unfed adult salivary gland cDNA library ISUF

text: Phage

text: Lambda TriplEx2


text: live

text: Generic

text: adults 18-24 hours days after attachment to a rabbit

text: salivary glands

text: Ixodes scapularis

characteristic: organism

text: female

text: Adult female salivary gland cDNA library IS18-24

text: Phage

text: Lambda TriplEx2


text: live

text: Generic

text: adults 6-12 hours days after attachment to a rabbit

text: salivary glands

text: Ixodes scapularis

text: female

text: Adult female salivary gland cDNA library IS6-12

characteristic: title

text: Phage

text: Lambda TriplEx2


text: Universite de Montreal

text: live

text: Generic

text: Depericarped ovaries were isolated from a compatible cross between self-incompatible accessions having the S12S14 self-incompatibility alleles (G4 line as female) and S11S13 self-incompatibility alleles (V22 line as male). Those two lines were derived from the parental lines PI 458314 (which carries the S11 and S12 self-incompatibility alleles) and line PI 230582 (which carries the S13 and S14 alleles) obtained through the Potato Introduction Station (Sturgeon Bay, WI). Plants were grown in greenhouses under natural light, fruit collection took place in the morning during summer (approx. 14h daylight), and fruits were pooled from different plants. Total RNA was isolated as described in EMBO J. 4: 2411-2418, 1985. The poly A+ RNA was purified from the total RNA by affinity chromatography using oligo dT agarose beads type VII (Amersham). Mean clone length of the library was 1,5 kb.

text: Zygotic to globular embryo

text: SOLR

text: Depericarped ovary/ovules with placenta tissue

text: Solanum chacoense

text: 2-6 days post-pollination ovaries

text: Plasmid

text: pBS SK


text: Universite de Montreal

text: live

text: Generic

text: Ovules were isolated from a compatible cross between self-incompatible accessions having the S12S14 self-incompatibility alleles (G4 line as female) and S11S13 self-incompatibility alleles (V22 line as male). Those two lines were derived from the parental lines PI 458314 (which carries the S11 and S12 self-incompatibility alleles) and line PI 230582 (which carries the S13 and S14 alleles) obtained through the Potato Introduction Station (Sturgeon Bay, WI). Plants were grown in greenhouses under natural light, fruit collection took place in the morning during summer (approx. 14h daylight), and fruits were pooled from different plants. Total RNA was isolated as described in EMBO J. 4: 2411-2418, 1985. The poly A+ RNA was purified from the total RNA by affinity chromatography using oligo dT agarose beads type VII (Amersham). Mean clone length of the library was 1,5 kb.

text: Globular to walking stick embryo

text: SOLR

text: Ovule (hand dissected)

text: Solanum chacoense

text: 7 to 17 days post-pollination ovules

text: Plasmid

text: pBS SK


text: OriGene Technologies, Inc. ( www.origene.com )

text: live

text: Generic

text: Oligo-dT primed reverse transcription optimized for large and GC rich mRNA transcripts, cDNA size selection, optimized ligation for large inserts into mammalian expression vector, random clones selected for end sequence verification of full-length genes

text: Mammary gland (cancer tissue)

text: Homo sapiens

text: Xho1/Sal1 compatible end ligatio

text: Breast cancer

text: Human breast cancer tissue, large insert, pCMV expression library

text: Plasmid


text: live

text: Generic

text: Fetal Brain

text: Homo sapiens

text: Xho1/Sal1 compatible end ligatio

text: Fetal Brain

text: Human fetal brain, large insert, pCMV expression library

text: Plasmid


text: live

text: Generic

text: Hippocampus

text: Homo sapiens

text: Xho1/Sal1 compatible end ligatio

text: Hippocampus

text: Human hippocampus, large insert, pCMV expression library

text: Plasmid


text: live

text: Generic

text: Oligo-dT primed reverse transcription optimized for large and GC rich mRNA transcripts, cDNA size selection, optimized ligation for large inserts into mammalian expression vector, random clones selected for end sequence verification of full-length genes; constructed and available from OriGene Technologies

text: Small intestine

text: Homo sapiens

text: Xho1/Sal1 compatible end ligatio

text: Small intestine

text: Human small intestine, large insert, pCMV expression library

text: Plasmid


text: Cold Spring Harbor Laboratory

text: live

text: Generic

text: Stratagene ZAP Express cDNA Synthesis Kit. The library was size-fractionated to enrich for large inserts.

text: megasporophyll

text: Ginkgo biloba

text: XhoI

text: Eco RI

text: Female

text: Ginkgo megasporophyll (NYBG)

text: Plasmid

text: pBK-CMV


text: Cold Spring Harbor Laboratory

text: live

text: Generic

text: microsporophyll

text: Ginkgo biloba

text: XhoI

text: Eco RI

text: male

text: Ginkgo microsporophyll (NYBG)

text: Plasmid

text: pBK-CMV


text: University of Waterloo

text: live

text: Generic

text: Differential display PCR performed on UV-B exposed plants. Total RNA was reverse transcribed and amplified using two 18mer arbitrary primers. Fragments between 250-600bp were ligated into pGEM T Easy and later sequenced.

text: Whole Plant

text: Lemna gibba

characteristic: strain

text: PCR cDNA Library UV-B exposed plants

text: pGEM T Easy


text: University of British Columbia

text: live

text: Generic

characteristic: cultivar

text: Adult white pine weevils (Pissodes strobi) were caged with mesh bags on sapling trees. Bark tissue with phloem and cambium attached was harvested 2 hours, 6 hours and 48 hours after continuous feeding by the insects. mRNA was isolated from each tissue source independently and equal quantities of mRNA from each tissue were then pooled. cDNA was prepared from 20 micrograms of mRNA according to the full-length cDNA library construction method described by Carninci P. et al. (2000), Genome Research 10(10):1617-1630 and directionally ligated into the pBluescript II SK (+) XR vector digested with XhoI (5' end) and BamHI (3'). Plasmid DNA was then transformed by electroporation into DH10B cells (Invitrogen) for propagation.

text: E. coli DH10B cells

characteristic: lab-host

text: Bark (with phloem and cambium attached) from two year old clonal trees grown under greenhouse conditions in standard potting soil mixture

text: Picea sitchensis

text: XhoI (5' end of cDNA)

text: BamHI (3' end of cDNA)

text: Hermaphrodite

text: pBluescript II SK (+) XR


text: University of British Columbia

text: live

text: Generic

text: s. mRNA was isolated from each tissue source independently and equal quantities of mRNA from each tissue were then pooled. cDNA was prepared from 20 micrograms of mRNA according to the full-length cDNA library construction method described by Carninci P. et al. (2000), Genome Research 10(10):1617-1630 and directionally ligated into the pBluescript II SK (+) XR vector digested with XhoI (5' end) and BamHI (3'). Plasmid DNA was then transformed by electroporation into DH10B cells (Invitrogen) for propagation.

text: E. coli DH10B cells

text: Green portion of the leader tissue from one year old clonal trees grown under greenhouse conditions in standard potting soil mixture

text: Picea sitchensis

text: XhoI (5' end of cDNA)

text: BamHI (3' end of cDNA)

text: Hermaphrodite

characteristic: sex

text: pBluescript II SK (+) XR


text: The Institute for Genomic Research

text: live

text: Generic

text: 31 from Mother Tree 7-56

characteristic: cell type

text: 7-56 mother tree, open-pollinated tree from, Lyons, Georgia, USA

text: Library enriched in transcripts present in suspension culture of Loblolly Pine (Pinus taeda L.), 7-56. Suspension culture was established from fertilized megagametophytes, according to method of Pullman et al 2003 (Pullman, G., S. Johnson, G. Peter, J. Cairney, and N. Xu. 2003. Improving loblolly pine somatic embryo maturation: Comparison of somatic and zygotic embryo morphology, germination, and gene expression. Plant Cell Reports 21:747-758. ) This suspension cell culture, which contains early developing embryos and undifferentiated embryogeneic tissue, was the source of the RNA used for the suspension culture library. Subtracted from this was cDNA made from a pool of RNA from zygotic embryos and female gametophytes from all stages of development plus somatic embryos from developmental stages beyond susepension culture (see Ciavatta VT, Morillon R, Pullman GS, Chrispeels M, Cairney J. 2001. An aquaglyceroporin is abundantly expressed early in the development of the suspensor and the embryo proper of loblolly pine (Pinus taeda L.). Plant Physiol. 127: 1556-1567 [http://www.plantphysiol.org/cgi/content/full/127/4/1556]) .

text: Suspension Culture Somatic Embryos (Stages 1-3)

text: Pinus taeda

text: EcoRI

text: ClaI

characteristic: tissue-type

text: Normalized pine embryo library, Lib_D

text: plasmid

characteristic: vector


characteristic: External Id

text: National Institute of Allergy and Infectious Diseases

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: The PCR-based cDNA library was made following the instructions for the SMART cDNA library construction kit (Clontech, Palo Alto, CA, USA) exactly as described Francischetti, I.M., Valenzuela, J.G., Pham, V.M., Garfield, M.K. & Ribeiro JM. (2002) J Exp Biol. 205, 2429-51

text: 2-3 day old adult

text: corpora allata plus corpora cardiaca

text: Aedes aegypti

text: female

text: Rockfeller

text: Aedes aegypti adult female corpora allata cDNA library AE_AEGY_CALLATA

text: Phage


characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: 2-3 day old adult

text: corpora allata plus corpora cardiaca

text: Anopheles albimanus

text: female

text: Corpora allata plus corpora cardiaca plus contaminant tissues

text: Anopheles albimanus adult female corpora allata cDNA library AN_ALBI_CALLATA

text: Phage


text: USDA/ARS

text: live

text: Generic

characteristic: NCBI submission package

text: 1-week after germination

characteristic: dev-stage

text: Young seedlings

text: Sorghum bicolor

text: Sorghum bicolor greenbug-responsive cDNA library


text: University of Nevada

text: live

text: Generic

text: Cabernet Sauvignon

text: A cDNA library from postveraison fruit at mixed stages 36-38 (Brix > 15) of the modified E-L system. Tissue collected from field grown plants. A description of the modified E-L system can be found in the paper by B. G. Coombe 'Adoption of a system for identifying grapevine growth stages' (1995) Aust. J. Grape and Wine Res. 1: 104-110.

characteristic: description

text: mixed 36-38 - modified E-L system (Brix > 15)

text: Fruit with seeds removed

characteristic: organ

text: Vitis vinifera

characteristic: organism

text: EcoRI

text: XhoI

text: Hermaphrodite

text: CabSau Berry Postveraison Mixed Stages 36-38 (Brix > 15) under water deficit stress

text: Phagemid

text: Lambda Uni-Zap XR, Bluescript SK-


text: Institute of Plant Physiology of Shanghai Institutes for Biological Sciences, The Chinese Acadamy of Sciences

text: live

text: Generic

characteristic: cultivar

text: -3 to 25 days post anthesis

text: Fiber and Ovule

text: Gossypium hirsutum

text: Fiber and Ovule of Xu-142 Lambda Zap Express Library

characteristic: title

text: Phage


text: University of Verona

text: live

text: Generic

text: aurea

text: whole plant

text: Brassica juncea

text: Brassica juncea cDNA-AFLP library


text: National Center for Genetic Engineering and Biotechnology (BIOTEC)

text: live

text: Generic

text: The cDNA library was generated as part of Penaeus monodon EST project. Total RNA was isolated from hematopoietic tissue of Penaeus monodon. The mRNA was further purified, reverse transcribed and cloned to Uni-ZAP XR.

text: hematopoietic tissue

text: Penaeus monodon

text: EcoR I

text: Xho I

text: Hematopoietic cDNA library

text: Phage

text: Uni-ZAP XR


text: Yonsei University College of Medicine

text: live

text: Generic

text: adult

text: Skeletal Muslce

text: Rattus norvegicus

text: male

characteristic: sex

text: Sprague-Dawley

characteristic: strain

text: soleus muscle

text: Rat soleus muscle

text: pGEM-T vector


text: BC Cancer Agency

text: live

text: Generic

text: 8.5 months

text: ElectroMAX DH10B T1 Phage-Resistant Cells

characteristic: lab-host

text: Hypothalamus

text: Bos taurus

text: SalI (5' end of cDNA)

text: NotI (3' end of cDNA)

text: female

text: Hereford

text: Hypothalamus

characteristic: tissue-type

text: pCMV SPORT 6.0


characteristic: External Id

text: University of Alberta

text: live

text: Generic

text: 8.5 months

text: Heart ventrical

text: Bos taurus

text: SalI (5' end of cDNA)

text: NotI (3' end of cDNA)

text: female

text: Hereford

text: Heart ventrical

text: pCMV SPORT 6.0

characteristic: vector


text: BC Cancer Agency

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: 6 months

text: Fetal liver

text: Bos taurus

text: SalI (5' end of cDNA)

text: NotI (3' end of cDNA)

text: male fetus

text: Hereford

text: Fetal liver

text: pCMV SPORT 6.0


text: Institiute of Genetic and Biophysic A.Buzzati Traverso 'IGB'

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: poly(A)RNA prepared from 200 pooled organs of Corti by Edward and al.

text: organ of Corti

text: Cavia porcellus

text: SalI

text: NotI

text: microdissected guinea pig organ of Corti

text: Plasmid


text: BC Cancer Agency

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: 6 months old fetus

text: E. coli DH10B T1 Phage Resistant

text: Ascending colon

text: Bos taurus

text: Blunt (5' end of cDNA)

text: NotI (3' end of cDNA)

text: male

text: L1 Hereford

text: Ascending colon

text: pExpress 1


text: Institut National de la Recherche Agronomique

text: live

text: Generic

text: The cDNA library was made as described in Helvig, C., Koener, J.,Unnithan, J.C. & Feyereisen, R. (2004) Proc. Natl. Acad. Sci. USA 101:4024-4029

characteristic: description

text: 3-5 day old adult mated females

characteristic: dev-stage

text: corpora allata

text: Diploptera punctata

text: female

text: Diploptera punctata corpora allata cDNA library DP_CALLATA

text: Plasmid

text: pSport I


text: National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency

text: live

text: Generic

text: Differentially expressed transcripts between ovaries and testes of Penaeus monodon identified through RAP-PCR, cloned and sequenced

text: ovaries and testes

characteristic: organ

text: Penaeus monodon

characteristic: organism

text: Differentially expressed RAP-PCR fragments


text: Royal Institute of Technology, KTH

text: live

text: Generic

text: Epithelial cells

characteristic: cell type

text: DNA in phagemid excised from Uni-ZAP XR vector.

text: Cell culture

text: Chironomus tentans

text: XhoI

text: EcoRI

text: Embryonal cell culture

text: C. tentans tissue culture cDNA library

characteristic: title

text: Phagemid

text: pBluescript


text: USDA/ARS

text: live

text: Generic

text: RNA from Yersinia ruckeri bath-challenged and sham-challenged fish was pooled from spleen and anterior kidney samples collected on days 1,3,5,7 and 10 post-challenge

text: anterior kidney and spleen

text: Oncorhynchus mykiss

text: leuk

text: plasmid


text: Institute of Bioorganic Chemistry PAS

text: live

text: Generic

text: EMIR

characteristic: cultivar

text: cDNA was isolated from infected lupin roots, collected 7-, 14- and 21 days after inoculation with Bradyrhizobium japonicum bv. genistaerum WM9 and normalized according to Ko (Nucleic Acids Res. 1990 18: 5705-5711) but without DNA fragmentation. Normalized cDNA without fractioning was cloned into phagemid ZAP II XR (EcoRV site) and converted into plasmid library.

text: roots with nodules

text: Lupinus angustifolius

text: Narrow leafed lupin normalized cDNA library

text: Plasmid

text: pBluescript


text: CIBNOR

text: live

text: Generic

text: Adult

text: Midgut gland

text: Penaeus vannamei

text: White shrimp hepatopancreas


text: The Institute for Genomic Research

text: live

text: Generic

text: Subjected to normalization

text: Adult

text: Fat body from immune stimulated hosts

text: Glossina morsitans morsitans

text: EcoRI

text: NotI

text: Male and female

characteristic: sex

text: Glossina morsitans morsitans Fat body cDNA


text: National Institute of Allergy and Infectious Diseases

text: live

text: Generic

text: unidirectional library was constructed using long distance PCR (Smart cDNA kit) and Lambda TriplEx2 vector

text: adult female

text: salivary glands

text: Anopheles darlingi

text: female

text: Adult female salivary gland cDNA library Adarg1

text: Phage

text: Lambda TriplEx2


text: Universite Libre de Bruxelles

text: live

text: Generic

text: ir et al., 1999). Transcript profiles of roots of plants grown in control medium or supplemented with 10 ´M CdSO4 for 72 hours were compared. Transcript derived fragments (TDFs), the abundance of which correlated with Cd-tolerance character, were cut from AFLP gels, amplified by PCR using Taq DNA polymerase, subsequently cloned in the pTZ57R/T vector (Fermentas GMBH) and sequenced. The A. halleri genotype originates from a population growing on a site highly contaminated with Zn, Cd and Pb located in the North of France (Bois des Asturies, Dpartement du Nord)

text: plants grown for 6 weeks in hydroponic culture

text: roots of A halleri plants grown in hydroponic cultures

text: Arabidopsis halleri subsp. halleri

text: XbaI

text: BamHI

text: whole root tissues

characteristic: tissue-type

text: Arabidopsis halleri CTR (Cadmium Tolerance Related) TDFs (Transcript Derived Fragments) collection

text: Plasmid

text: pTZ57R/T vector (InsT/AcloneTM PCR Product Cloning Kit; Fermentas)


characteristic: External Id

text: University of Cape Town

text: live

text: Generic

text: (Bak.) Dur and Schinz

text: 11k cDNA library pool of four individual libraries, representing the dehydrating and rehydrating states and prepared from roots and leaves i.e. root dehydration (RD), leaf dehydration (LD), root rehydration (RR) and leaf rehydration (LR)

text: E. coli DH10B

characteristic: lab-host

text: Roots & Leaves

text: Xerophyta humilis

text: EcoRI

text: XhoI

text: Xerophyta_humilis root and leaf mixed library

text: phagemid

text: pBluescript SK


text: University of Edinburgh

characteristic: INSDC center name

text: live

text: Generic

text: This library was prepared by Sarah Grant, Department of Biology, University of North Carolina at Chapel Hill (sgrant@email.unc.edu)

text: male flower

text: Silene latifolia

text: Silene latifolia male flower library

text: phage

text: lambda zap

characteristic: vector


text: University of Kentucky

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: 5'-end sequences from a subtracted cDNA library. The frequency of common equences in this library was reduced by screening filter lifts of colonies with complex probes

text: adult

text: olfactory organ; lateral ramus of first antennae

text: Homarus americanus

text: NotI

text: SalI

text: male and female

text: mature zone

text: Homarus EST library project

text: plasmid

text: pBluescript


text: National Livestock Research Institute, RDA

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: adipocyte

text: 24 months old

text: XL1-BlueMRF' strain

text: adipose tissue

text: Bos taurus

text: EcoRI

text: Xho I

text: Four males mixed

text: Bos taurus CF-24-HW fat cDNA library

text: Phage

text: Uni-ZAPXR


text: live

text: Generic

characteristic: NCBI submission package

text: hepatocyte

text: 24 months old

text: XL1-BlueMRF' strain

text: liver tissue

text: Bos taurus

text: EcoRI

text: Xho I

text: Four males mixed

text: Bos taurus CF-24-HW liver cDNA library

text: Phage

text: Uni-ZAPXR


text: live

text: Generic

text: myocyte

text: 24 months old

characteristic: dev-stage

text: XL1-BlueMRF' strain

text: skeletal muscle

text: Bos taurus

text: EcoRI

text: Xho I

text: Four males mixed

text: Bos taurus CF-24-HW loin cDNA library

text: Phage

text: Uni-ZAPXR


text: University of Alberta

text: live

text: Generic

text: 6 months

text: ElectroMAX DH10B T1 Phage-Resistant Cells

text: Fetal Medulla

characteristic: organ

text: Bos taurus

text: SalI (5' end of cDNA)

text: NotI (3' end of cDNA)

text: male

text: Hereford

characteristic: strain

text: Fetal Medulla

text: pCMV SPORT 6.0


text: University of Alberta

text: live

text: Generic

text: 6 months

text: Fetal Cerebellum

text: Bos taurus

characteristic: organism

text: SalI (5' end of cDNA)

text: NotI (3' end of cDNA)

text: male

text: Hereford

text: Fetal Cerebellum

text: pCMV SPORT 6.0


text: BC Cancer Agency

text: live

text: Generic

text: 8.5 months old calf

text: E. coli DH10B T1 Phage resistant

text: Hippocampus

text: Bos taurus

text: Blunt (5' end of cDNA)

text: NotI (3' end of cDNA)

text: female

text: L1 Hereford

text: Hippocampus

characteristic: title

text: pExpress 1


text: BC Cancer Agency

text: live

text: Generic

text: 6 months

text: Fetal spinal column

text: Bos taurus

text: SalI (5' end of cDNA)

text: NotI (3' end of cDNA)

text: male

text: Hereford

text: Fetal spinal column

text: pCMV SPORT 6.0


text: BC Cancer Agency

text: live

text: Generic

text: 6 months

text: Fetal cerebral cortex

text: Bos taurus

text: SalI (5' end of cDNA)

text: NotI (3' end of cDNA)

text: male

text: Hereford

text: Fetal cerebral cortex

text: pCMV SPORT 6.0


text: BC Cancer Agency

text: live

text: Generic

text: 8.5 months old calf

text: E. coli DH10B T1 Phage resistant

text: Cerebral cortex

text: Bos taurus

text: Blunt (5' end of cDNA)

text: NotI (3' end of cDNA)

text: female

text: L1 Hereford

text: Cerebral cortex

text: pExpress 1


text: CSIRO

text: live

text: Generic

text: Cabernet Sauvignon

characteristic: cultivar

text: tion of a system for identifying grapevine growth stages (1995) Aust. J. Grape and Wine Res. 1: 104-110.

characteristic: description

text: 31 - modified E-L system

text: Inflorescence

text: Vitis vinifera

text: Hermaphrodite

text: CabSau Immature Inflorescence Stage 31 (INFu0031)

text: Plasmid


text: University of California

text: live

text: Generic

text: Total accessory gland RNA was isolated using the Ambion mirVana miRNA kit and was then RNAsed. cDNA was synthesized (Invitrogen GeneRacer kit; the SSIII module and oligo(dT) primer were used for the RT step), amplified (8 cycles) using the Roche Expand High Fidelity PCR System, purified (Qiagen QIAquick PCR Purification kit), incubated in Promega Taq polymerase and ligated into PCR4 TOPO vector

text: Adult

text: Accessory Gland

text: Drosophila erecta

text: Male

characteristic: sex

text: Male Accessory Gland Library

text: Plasmid


characteristic: External Id

text: University of California

text: live

text: Generic

text: Total accessory gland RNA was isolated using the Ambion mirVana miRNA kit and was then RNAsed. cDNA was synthesized (Invitrogen GeneRacer kit; the SSIII module and oligo(dT) primer were used for the RT step), amplified (5 cycles) using the Roche Expand High Fidelity PCR System, purified (Qiagen QIAquick PCR Purification kit), incubated in Promega Taq polymerase and ligated into PCR4 TOPO vector

text: Adult

text: Accessory Gland

text: Drosophila yakuba

text: Male

text: Male Accessory Gland Library Drosophila yakuba

text: Plasmid


text: USDA-ARS

text: live

text: Generic

text: E. coli

characteristic: lab-host

text: fiber initials

text: Gossypium hirsutum

text: Young Fiber 1-3 days after anthesis

text: Topo TA


text: Advanced Center for Genome Technology, University of Oklahoma

characteristic: INSDC center name

text: live

text: Generic

text: early blastemal (4 days post autotomy) Celuca pugilator cDNA library created by Dr. David Durica at the University of Oklahoma, Department of Zoology, Norman, Oklahoma.

text: older than 3 month

text: regenerating buds

text: Leptuca pugilator

text: EcoRI

text: XhoI

text: male

text: early blastemal (4 days post autotomy)

characteristic: tissue-type

text: Fiddler crab 4 day blastema cDNA library

text: phagemid

text: pBlueScriptSK(-) (Stratagene)


text: USDA-ARS

characteristic: INSDC first public

text: live

text: Generic

text: derived from membrane-bound polyribosomal mRNA

text: E. coli

text: fiber initial

text: Gossypium hirsutum

text: Fiber - 1 day after anthesis

text: Topo TA

characteristic: vector


text: University of Adelaide

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Atlas, Atlas 46

text: Abaxial epidermis was peeled off 24 hours after inoculation with Rhynchosporium secalis or treatment with the avirulence protein NIP1. Suppression subtractive hybridizations were carried out using cDNA populations from incompatible and compatible interactions.

text: Primary leaves

text: Hordeum vulgare subsp. vulgare

text: Epidermis

text: Subtracted libraries from epidermis of 7 day old barley plants inoculated with R. secalis or the avirulence protein NIP1


text: Universitaet Hamburg, Biozentrum Klein Flottbek

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: central cell

characteristic: cell type

text: Central Cells were isolated by manual microdissection after a brief maceration of the nucellus tissue. The cDNA library was constructed with the SMART cDNA library construction kit.

text: mature

text: female gametophyte

text: Zea mays

text: Zea mays central cell cDNA library

text: TriplEx2, Clontech


text: University of Victoria

text: live

text: Generic

text: ssalplnb mixed_tissue Salmo salar cDNA ; Tissue contributors: Robert Devlin (DFO, Vancouver, B.C.)

text: Pyloric Caecum

characteristic: organ

text: Salmo salar

text: McConnell

characteristic: strain

text: plnb

text: BlueScript


text: University of Victoria

text: live

text: Generic

text: ssalrgb2 mixed_tissue Salmo salar cDNA; Tissue contributors: Robert Devlin (DFO, Vancouver, B.C.)

text: brain, kidney, spleen

text: Salmo salar

characteristic: organism

text: McConnell

text: mixed tissue

characteristic: title


text: Universidade do Estado do Rio de Janeiro

text: live

text: Generic

text: Poly(A)-rich RNA was prepared from total RNA of the venom gland by oligo(dT)-cellulose chromatography. A directional cDNA library was constructed using a plasmid cloning kit (Superscript plasmid system, Life Technologies Inc.), as described previously (Arocas et al., 1997).

characteristic: description

text: Adult

characteristic: dev-stage

text: Venom gland

text: Bothrops jararaca

text: EcoRI/NotI/BAP

text: female

text: phagemid

text: pT7T3D EcoRI/NotI/BAP phagemid vector (Pharmacia LKB Biotechnology Inc.)


characteristic: External Id

text: National Institute of Allergy and Infectious Diseases

text: live

text: Generic

text: Secretory

text: PCR-based cDNA library made with SMART cDNA library construction kit from Clontech

text: Adult

text: Salivary gland

text: Aedes aegypti

text: Female

characteristic: sex

text: Rockfeller

text: Salivary gland

text: Aedes aegypti adult female salivary glands

text: Phage

text: Lambda TriplEx2


text: The Biology Department of Shandong Normal University

characteristic: INSDC center name

text: live

text: Generic

text: total RNA extraction from normal growing Limonium sinense by RNAgent kit(Promega); mRNA isolation by MESSAGEMAKER kit(GIBCO BRL); directional cDNA synthesis(EcoRI XhoI) by cDNA synthesis kit(Invitrogen); ***the Superscript Plasmid System for cDNA synthesis and cloning kit (Invitrogen, Carlsbad, CA, USA)

text: seedling

text: aerial part tissue

text: Limonium sinense

text: EcoRI

text: XhoI

text: halophyte Limonium sinense plasmid cDNA library

text: plasmid

text: pBluescript II SK(+) XR


text: Temasek Lifesciences Laboratory

text: live

text: Generic

text: cDNA was synthesized from 60-day-old carp testis total RNA using SMART cDNA library construction kit (Clontech). SfiI-digested and size fractionated cDNA fragments (on average 1-1.5 kb in length) were ligated into pBS-SK-Sfi vector (a pBluescript-SK based vector with Sfi I sites) and transformed into XL10-Gold competent cells (Stratagene). The insert from randomly selected white colonies was PCR-amplified using M13 forward and reverse primers and partially sequenced by M13 reverse primer.

text: 60 days post fertilization

text: developing testis

text: Cyprinus carpio

text: male

text: all-male (XY) offspring of XX females and androgenetic YY males

text: 60-day-old common carp testis (full-length)

text: plasmid

text: pBS-SK-Sfi


text: Temasek Lifesciences Laboratory

text: live

text: Generic

text: cDNA was synthesized from 80-day-old carp testis total RNA using SMART cDNA library construction kit (Clontech). SfiI-digested and size fractionated cDNA fragments (on average 1-1.5 kb in length) were ligated into pBS-SK-Sfi vector (a pBluescript-SK based vector with Sfi I sites) and transformed into XL10-Gold competent cells (Stratagene). The insert from randomly selected white colonies was PCR-amplified using M13 forward and reverse primers and partially sequenced by M13 reverse primer.

text: 80 days post fertilization

text: developing testis

text: Cyprinus carpio

text: male

text: 80-day-old common carp testis (full-length)

text: plasmid

text: pBS-SK-Sfi


text: Temasek Lifesciences Laboratory

text: live

text: Generic

text: cDNA was synthesized from 100-day-old carp testis total RNA using SMART cDNA library construction kit (Clontech). SfiI-digested and size fractionated cDNA fragments (on average 1-1.5 kb in length) were ligated into pBS-SK-Sfi vector (a pBluescript-SK based vector with Sfi I sites) and transformed into XL10-Gold competent cells (Stratagene). The insert from randomly selected white colonies was PCR-amplified using M13 forward and reverse primers and partially sequenced by M13 reverse primer.

text: 100 days post fertilization

text: developing testis

text: Cyprinus carpio

text: male

text: 100-day-old common carp testis (full-length)

text: plasmid

text: pBS-SK-Sfi


text: Temasek Lifesciences Laboratory

text: live

text: Generic

text: cDNA was synthesized from 70 and 100-day-old carp testis total RNA using SMART PCR cDNA synthesis kit (Clontech). PCR-SelectTM cDNA subtraction kit (Clontech) was used to enrich for fragments, which were present in the 70 day cDNA but not in the 100 day sample. The selectively amplified cDNA fragments (on average 400-800bp in length) were ligated into pGEM-T-Easy (Promega) and transformed into XL10-Gold competent cells (Stratagene). The insert from randomly selected white colonies was PCR-amplified using M13 forward and reverse primers and partially sequenced by M13 reverse primer.

text: 70 days post fertilization

text: developing testis

text: Cyprinus carpio

text: male

text: 70-day-old common carp testis (subtracted)

text: plasmid

text: pGEM-T-Easy


text: Temasek Lifesciences Laboratory

text: live

text: Generic

text: cDNA was synthesized from 70 and 100 day old carp testis total RNA using SMART PCR cDNA synthesis kit (Clontech). PCR-SelectTM cDNA subtraction kit (Clontech) was used to enrich for fragments, which were present in the 100 day cDNA but not in the 70 day sample. The selectively amplified cDNA fragments (on average 400-800bp in length) were ligated into pGEM-T-Easy (Promega) and transformed into XL10-Gold competent cells (Stratagene). The insert from randomly selected white colonies was PCR-amplified using M13 forward and reverse primers and partially sequenced by M13 reverse primer.

text: 100 days post fertilization

text: developing testis

text: Cyprinus carpio

text: male

text: 100 day old common carp testis (subtracted)

text: plasmid

text: pGEM-T-Easy


text: The University of Reading, School of Biological Sciences

text: live

text: Generic

text: ESTs obtained through suppressive subtractive hybridisation were cloned in TOPO chemically competent E. coli cells

text: Juvenile (>48 h)

text: Whole organism

text: Daphnia magna

text: female

text: Clone type 5 (IRCHA)

text: Whole organism

characteristic: tissue-type

text: Daphnia magna SSH Ibuprofen Library

text: Plasmid

text: pCR 4-TOPO


text: Baylor College of Medicine

characteristic: INSDC first public

text: live

text: Generic

text: A hematopoietic stem cell gene enriched library prepared by Suppression Subtractive Hybridization of side population cells from bone marrow subtracted with whole bone marrow cells. cDNAs were fragmented, concatenated, cloned, sequenced, and electronically de-concatenated.

text: 8 week old

text: Bone Marrow

text: Mus musculus

text: CST-HSC


text: Baylor College of Medicine

text: live

text: Generic

text: A hematopoietic stem cell gene depleted library prepared by Suppression Subtractive Hybridization of whole bone marrow cells subtracted with side population cells from bone marrow. Prepared as a control for the CST-HSC library. cDNAs were fragmented, concatenated, cloned, sequenced, and electronically de-concatenated.

text: Bone Marrow

text: Mus musculus

text: CST-BM


text: Baylor College of Medicine

text: live

text: Generic

text: A stem cell library enriched for genes expressed in quiescent hematopoietic stem cells. Prepared by Suppression Subtractive Hybridization of quiescent side population cells from bone marrow subtracted with proliferating side population cells from bone marrow. cDNAs were fragmented, concatenated, cloned, sequenced, and electronically de-concatenated.

text: Bone Marrow

text: Mus musculus

text: CST-QuHSC


text: Baylor College of Medicine

text: live

text: Generic

text: A stem cell library enriched for genes expressed in proliferating hematopoietic stem cells. Prepared by Suppression Subtractive Hybridization of proliferating side population cells from bone marrow subtracted with quiescent side population cells from bone marrow. cDNAs were fragmented, concatenated, cloned, sequenced, and electronically de-concatenated.

text: Bone Marrow

text: Mus musculus

text: CST-ProHSC


text: Instituto Butantan

text: live

text: Generic

text: es (e-value < e-05).

text: venom glands

text: Lachesis muta

text: EcoR I

text: Not I

text: venom glands

text: Snake Lachesis muta cDNA library LMA

text: Plasmid

characteristic: vector


text: Centre National de la Recherche Scientifique (CNRS)

characteristic: INSDC last update

text: live

text: Generic

text: young, developing, 4 to 8 days post infection with Sinorhizobium meliloti strain Sm41

text: root nodule

text: Medicago truncatula

text: EcoRI

text: XhoI

text: NOLLY

text: Lambda HybriZAP


text: Insitute of Vegetable Science, Zhejiang University of China

text: live

characteristic: INSDC status

text: Generic

characteristic: cultivar

text: pollen development

text: flower buds

text: Brassica rapa

text: buds

text: cDNA of flower buds


text: University of Antwerp

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Hepatopancreas

text: Cyprinus carpio

text: Carp Hepatopancreas Cadmium Related cDNA Library


text: Waksman Institute, Rutgers University

text: live

text: Generic

text: A. franciscana cDNAs were reversed transcribed from polyA-selected mRNAs. EcoRI linkers were ligated to the cDNAs, which were subsequently ligated into the unique EcoRI site of plasmid vector pTriplEx2. Recombinant plasmids were transformed into E. coli strain DH5alpha.

text: Mixed stages

text: E. coli DH5alpha

characteristic: lab-host

text: Whole animal

characteristic: organ

text: Artemia franciscana

text: EcoRI

text: Mixed male and female

text: WSSP A. franciscana cDNA Library

text: Plasmid


text: University of Victoria

text: live

text: Generic

text: Tissue contributors: Robert Devlin (DFO, Vancouver, B.C.)

text: Pyloric Caecum

text: Salmo salar

characteristic: organism

text: McConnell

characteristic: strain

text: ssalplnb mixed_tissue Salmo salar cDNA

text: BlueScript


text: University of Victoria

text: live

text: Generic

characteristic: description

text: brain, kidney, spleen

text: Salmo salar

text: McConnell

text: mixed tissue

text: ssalrgb2 mixed_tissue Salmo salar cDNA

characteristic: title


characteristic: External Id

text: Guangdong Academy of Agricultural Sciences

text: live

text: Generic

text: A subtracted skeletal muscle library was constructed using CLONTECH PCR-Select cDNA Subtraction Kit between Chinese Langtang Pig(tester) and Large White(driver). Real time PCR was conducting to testify differential expression between two breeds.

text: Adult pig

characteristic: dev-stage

text: Skeletal Muscle

text: Sus scrofa

text: Chinese Langtang Pig and Large White

text: musculus longissimus dorsi

text: Porcine subtracted plasmid Library 1

text: T vector PMD 18-T(TAKARA)


characteristic: INSDC center name

text: live

text: Generic

text: A subtracted skeletal muscle library was constructed using CLONTECH PCR-Select cDNA Subtraction Kit between Chinese Langtang Pig(driver) and Large White(tester). Real time PCR was conducting to testify differential expression between two breeds.

text: Adult pig

text: Skeletal Muscle

text: Sus scrofa

text: Chinese Langtang Pig and Large White

text: musculus longissimus dorsi

text: Porcine subtracted plasmid Library 2

text: T vector PMD 18-T(TAKARA)


text: live

text: Generic

text: This cDNA library was constructed using CLONTECH 'SMART cDNA Library Construction Kit'.The sample for the library was taken from a adult male Langtang pig, a Chinese native breed in Guangdong.

text: At the age of 150 days (about 70Kg bodyweight)

text: Skeletal Muscle

text: Sus scrofa

text: SfiI A

text: SfiI B

text: male

characteristic: sex

text: Chinese Langtang Pig

text: musculus longissimus dorsi

characteristic: tissue-type

text: Swine Lambda TriplEx2 Library

text: Lambda TriplEx2


text: NRC Institute for Marine Biosciences

characteristic: INSDC first public

text: live

text: Generic

text: normalised cDNA library generated using the Trimmer Direct kit (Evrogen)

text: adult

text: DH10B (T1 phage-resistant)

text: head kidney

text: Hippoglossus hippoglossus

text: SfiI

text: plasmid

text: pDNR-lib

characteristic: vector


text: Aquaculture Protein Centre/Norwegian School of Veterinary Science

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Suppression subtractive hybridization

text: distal intestine

text: Salmo salar

text: Salmobreed AS, Bergen, Norway

text: Atlantic salmon distal intestine SSH

text: plasmid


text: National Cancer Institute / NIH

text: live

text: Generic

characteristic: NCBI submission model

text: cDNA was primed using oligo-dT primer: GACTAGTTCTAGATCGCGAGCGGCCGCC(T)25 and cloned into the SmaI/NotI sites of pCS111. Size-selection >1.25 kb resulted in an average insert size of 1.25 kb. This primary library was constructed by Express Genomics (Frederick, MD). Note: this is a (http://xgc.nci.nih.gov/) Xenopus Gene Collection library.

text: DH10B TonA

text: olfactory bulbs

text: Xenopus laevis

text: SmaI

text: NotI

text: olfactory bulbs, pooled

text: plasmid


text: University of St Andrews

text: live

text: Generic

characteristic: NCBI submission package

text: The library was prepared by Fish Muscle Research Group, Gatty Marine Laboratory, School of Biology, University of St Andrews

text: 2-cell stage embryo

text: Hippoglossus hippoglossus

text: EcoR I

text: Xho I

text: 2-cell stage embryo(8 hours post fertilization(hpf)) of Atlantic halibut, Hippoglossus hippoglossus

text: plasmid

text: pBlueScript II SK(+) XR


text: The Third Military Medical University

text: live

text: Generic

text: The library was constructed by using suppression subtractive hybridization technique.

text: postnatal 67 days

text: DH5a E. coli

characteristic: lab-host

text: visual cortex

text: Rattus norvegicus

text: female, male

text: Lang-Evans

text: cortex

text: Rat Subtracted cDNA Library

text: Plasmid

text: PUCm-T vector


text: live

text: Generic

text: Suppression subtractive hybridization

text: distal intestine

characteristic: organ

text: Salmo salar

text: Aqua Gen breed

text: Atlantic salmon distal intestine SSH II

text: plasmid


text: 1. College of animal science, Zhejiang University, 2. College of Animal Science and Technology, Northwest Sci-Tech University of Agriculture and Forestry.

text: live

text: Generic

text: Shin Ichinose

characteristic: cultivar

text: Total RNA was extracted from growing buds of mulberry cultivar 'Shin Ichinose' and its mutant 'Fengwei Ichinose' by the method of Trizol (Invitrogen) in summer, 'Fengwei Ichinose' is a leaf mutant of 'Shin Ichinose' induced by gamma ray of Co60. Poly(A)+mRNA was isolated from total RNA using the PolyATtract mRNA Isolation system III (Promega). mRNA was reverse transcribed into double stranded cDNA using the SMART cDNA library construction kit (CLONTECH). Template preparation, PCR procedure and polyacrylamide gel electrophoresis of cDNA-AFLP was performed according the method described by Bachem (Plant Molecular Biology Reporter 16:157-173, 1998). The gels were performed using the silver staining. The different cDNA fragments between the wild type 'Shin Ichinose' and its mutant 'Fengwei Ichinose' were directionally cloned into the pUC19 T-vector. The ligated cDNA fragments were transformed into E. coli DH5alpha host cells and sequenced.

text: bud and young leaf

text: Morus alba

characteristic: organism

text: Growing bud library in mulberry leaf mutant by cDNA-AFLP

text: Plasmid


text: Cornell University

text: live

text: Generic

text: Several (n= 8) sexually mature males

text: E.coli ElectroMax DH10B (Invitrogen)

text: Accessory gland

text: Gryllus firmus

text: male

text: Natural population Parksley, VA USA

characteristic: strain

text: plasmid


text: Cornell University

text: live

text: Generic

text: several (n=11) sexually mature males

text: E. coli EletroMax DH5-alpha (Invitrogen)

text: Accessory gland

text: Gryllus pennsylvanicus

text: male

text: Natural population Ithaca, NY USA

characteristic: title

text: plasmid


characteristic: External Id

text: Agencourt Bioscience Corporation

text: live

text: Generic

text: NHGRI Large scale sequencing grant Drosophila erecta, EST library

characteristic: description

text: DH10B T1 phage resistant

text: pool of adult and embryo RNA from whole flies

text: Drosophila erecta

text: NotI

text: SmaI

text: D. erecta EST

text: plasmid


text: Agencourt Bioscience Corporation

characteristic: INSDC center name

text: live

text: Generic

text: NHGRI Large scale sequencing grant Drosophila virilis, EST library

text: DH10B T1 phage resistant

text: Drosophila virilis

text: NotI

text: SmaI

text: D. virilis EST

text: plasmid


text: Agencourt Bioscience Corporation

text: live

text: Generic

text: NHGRI Large scale sequencing grant Drosophila ananassae, EST library

text: DH10B T1 phage resistant

text: Drosophila ananassae

text: NotI

text: SmaI

text: D. ananassae EST

text: plasmid


text: Agencourt Bioscience Corporation

text: live

text: Generic

text: NHGRI Large scale sequencing grant Drosophila grimshawi, EST library

text: DH10B T1 phage resistant

text: Drosophila grimshawi

text: NotI

text: SmaI

text: D. grimshawi EST

text: plasmid


text: Agencourt Bioscience Corporation

text: live

text: Generic

text: NHGRI Large scale sequencing grant Drosophila mojavensis, EST library

text: DH10B T1 phage resistant

text: Drosophila mojavensis

text: NotI

text: SmaI

text: D. mojavensis EST

text: plasmid


text: Norweigian University of Life Sciences

text: live

text: Generic

text: Lillo (virus free)

text: RNAspektra (GenHunter, Nashville, TN). Total RNA from abscission zone pedicel tissue was reverse-transcribed using one-base-oligo dT primers, followed by PCR using these primers in combination with arbitrary 13-mers. Differentially expressed bands were reamplified using the same primers and cloned into the pCR 2.1 vector using the TA cloning kit (Invitrogen Corp., Carlsbad, CA) and sequenced.

text: E.Coli

text: flower pedicel

text: Euphorbia pulcherrima

text: HindIII

text: HindIII

text: abscission zone

text: Poinsettia flower pedicel abscission zone

text: Plasmid

text: pCR 2.1


text: Iowa State University

text: live

text: Generic

text: Nectary tissues from flowers at Stage 6 of development from mature greenhouse grown Nicotiana langsdorffii X Nicotiana sanderae (LxS8) plants

text: Floral stage 6

characteristic: dev-stage

text: Floral nectary

text: Nicotiana langsdorffii x Nicotiana sanderae

text: Sfi 1A

text: Sfi 1B

text: Ornamental tobacco (LxS8) Stage 6 Floral nectary cDNA library

text: plasmid

text: pDNR-LIB


text: Iowa State University

text: live

text: Generic

text: Nectary tissues from flowers at Stage 12 of development from mature greenhouse grown Nicotiana langsdorffii X Nicotiana sanderae (LxS8) plants

text: Floral stage 12

text: Floral nectary

text: Sfi 1A

text: Sfi 1B

text: Ornamental tobacco (LxS8) Stage 12 Floral nectary cDNA library

text: bacteriophage


text: Iowa State University

text: live

text: Generic

text: Nectary tissues from flowers at Stage Post-Fertilization (44 hours after fertilization) of development from mature greenhouse grown Nicotiana langsdorffii X Nicotiana sanderae (LXS8) plants

text: Mature nectaries 44 hours after fertilization

text: Floral nectary

text: Sfi 1A

text: Sfi 1B

text: Ornamental tobacco (LxS8) post-fertilization Floral nectary cDNA library

text: plasmid

text: pDNR-LIB


text: Iowa State University

text: live

text: Generic

text: Library was prepared using the PCR-Select method. Desired mRNA was isolated from floral nectary tissues at Stage 6 of development from mature greenhouse grown Nicotiana langsdorffii X Nicotiana sanderae (LxS8) plants. Control (driver) mRNA was harvested from floral nectary tissues at Stage 12 of development from mature greenhouse grown Nicotiana langsdorffii X Nicotiana sanderae (LxS8) plants. Following second strand synthesis of desired Stage 6 cDNA, the cDNAs were sheared to approximately 300 nucleotides in length. Subtraction was Stage 6 sequences minus Stage 12 sequences.

text: Floral stage 6

text: Floral nectary

text: Eco RI

text: Eco RI

text: Ornamental tobacco (LxS8) Stage 6 Floral Nectary subtracted cDNA library

text: plasmid


text: Agriculture Research Organization

text: live

text: Generic

text: First nymph and adults

text: Mustard (Tendergreen)

text: whole insects and heads

text: Myzus persicae

text: SfiIA

text: SfiIB

text: Female

characteristic: sex

text: Myzus persicae first nymph SMART cDNA library

text: Plasmid


text: Carnegie Institution of Washington

characteristic: INSDC first public

text: live

text: Generic

text: Subtractive hybridization was performed with the PCR-Select Subtraction Kit (Clontech), according to the manufacturerÕs protocol. Tester tissue consisted of whole-body 15 day post-hatch (dph) flounder larvae that had been treated with triiodothyronine (50nM) and methimazol (0.1M) from 12-15 dph. Driver tissue were treated with methimazol only.

text: 15 days post-hatch

text: Whole-body larvae

text: Paralichthys lethostigma

text: Rsa I

text: Rsa I

text: Larval flounder thyroid hormone (72 hrs) up-regulated mRNA

text: Plasmid

text: pCRII-TOPO


text: The University of Georgia, Department of Plant Biology

text: live

text: Generic

text: The library was made from poly-A RNA in the cloning vector lambda ZAP II. Clones to be sequenced were prepared by mass excision.

text: SOLR strain of E. coli

text: Embryos germinated for 24 hr

text: Sorghum bicolor

text: XhoI

text: EcoRI

text: Embryos

text: Plasmid

text: pBlueScriptSK-

characteristic: vector


characteristic: INSDC last update

text: live

text: Generic

text: Mature plants were placed in a growth chamber for 15 days with 16 hr darkness and 8 hr light (flowering is induced by short-day conditions); 16 days after being returned to the greenhouse under natural long days during late April/early May, meristems were harvested The library was made from poly-A RNA in the cloning vector lambda ZAP II. Clones to be sequenced were prepared by mass excision

text: SOLR strain of E. coli

text: Floral-induced meristems

text: Sorghum propinquum

text: XhoI

text: EcoRI

text: Floral-induced meristems

text: Plasmid

text: pBlueScriptSK-


text: live

characteristic: INSDC status

text: Generic

text: SOLR strain of E. coli

text: Developing preanthesis panicles

text: Sorghum bicolor

text: XhoI

text: EcoRI

text: Immature panicles

text: Plasmid

text: pBlueScriptSK-


text: live

text: Generic

characteristic: NCBI submission model

text: SOLR strain of E. coli

text: 10- to 14-day-old light-grown (greenhouse) seedlings

text: Sorghum bicolor

text: XhoI

text: EcoRI

text: Light-grown seedlings

text: Plasmid

text: pBlueScriptSK-


text: live

text: Generic

characteristic: NCBI submission package

text: SOLR strain of E. coli

text: Mix of ovaries of varying immature stages from 8-week-old plants

text: Sorghum bicolor

text: XhoI

text: EcoRI

text: Ovaries

text: Plasmid

text: pBlueScriptSK-


text: live

text: Generic

text: Two-week-old sorghum plants (BTX 623 cultivar) were infected with pathogen (isolate FRM42I of Colletotrichum graminicola, which is a sorghum isolate). RNA was prepared from infected leaves harvested from 45 seedlings 48 hours after inoculation. Note: young seedlings (2 weeks old) exhibit juvenile resistant reaction, which is an incompatible interaction. As they grow older (4 weeks or older), plants resume susceptiblity to anthracnose disease. The library was made from poly-A RNA in the cloning vector lambda ZAP II. Clones to be sequenced were prepared by mass excision. WARNING: While most or all ESTs are expected to derive from the host plant, no effort was made to eliminate ESTs deriving from the pathogen.

text: SOLR strain of E. coli

characteristic: lab-host

text: Anthracnose-infected leaves from two-week-old sorghum plants 48 hr after inoculation

text: Sorghum bicolor

text: XhoI

text: EcoRI

text: Pathogen-induced: incompatible

text: Plasmid

text: pBlueScriptSK-


text: live

text: Generic

text: SOLR strain of E. coli

text: Mix of 5-week-old plants on days 7 & 8 after water was withheld

characteristic: organ

text: Sorghum bicolor

text: XhoI

text: EcoRI

text: Drought-stressed

text: Plasmid

text: pBlueScriptSK-


text: Carnegie Institution of Washington

text: live

text: Generic

text: Subtractive hybridization was performed with the PCR-Select Subtraction Kit (Clontech), according to the manufacturerÕs protocol. Tester tissue consisted of whole-body 15 day post-hatch (dph) larvae that had been treated with methimazol (0.1M) from 12-15 dph. Driver tissue was treated with methimazol and triiodothyronine (50nM).

text: 15 days post-hatch

text: Whole-body larvae

text: Paralichthys lethostigma

text: Rsa I

text: Rsa I

text: Larval flounder thyroid hormone (72 hrs) down-regulated mRNA

text: Plasmid

text: pCRII-TOPO


text: Carnegie Institution of Washington

text: live

text: Generic

text: Differentially expressed genes from thyroid hormone treated and untreated larval flounder were obtained using Seegene's GeneFishing DEG Discovery service. mRNA was derived from whole-body 15 day post-hatch (dph) larvae treated with either methimazol (0.1M) or with methimazol + triiodothyronine (50nM) for 24 hrs.

text: 15 days post-hatch

text: Whole-body larvae

text: Paralichthys lethostigma

text: Larval flounder thyroid hormone (24 hrs) differentially-expressed mRNA

text: Plasmid

text: pCR2.1-TOPO or pCRII-TOPO


text: Universidad de Oviedo

text: live

text: Generic

text: SSH library constructed after 16 hours of adventitious shoot induction in Pinus pinea L. cotyledons cultured in presence of bencyladenine.

text: Mature embryo

text: Pinus pinea

text: Cotyledon

characteristic: tissue-type

text: Plasmid

text: pGEMT


text: Institute of Genetics and Developmental Biology, Chinese Academy of Sciences

text: live

text: Generic

text: Four-week-old Thellungiella halophila was collected after treated with NaCl concentration gradient (100 mM, 200 mM, 300 mM, 400 mM). Every 30 minutes, the plants were treated with NaCl from the lowest concentration to the highest. From the first treatment, every 15 minutes, the sample was collected together for RNA extraction until 1 hour after the treatment of 400 mM NaCl. The cDNA library was constructed by using cDNA synthesis kit (Stratagene).

text: seedling

text: leaves and roots

text: Eutrema halophilum

characteristic: organism

text: EcoRI

text: XhoI

text: Thellungiella halophila pGAD-GH cDNA library

text: plasmid

text: pGAD GH


text: China Agricultural University

text: live

text: Generic

text: Mar 51

characteristic: cultivar

text: Foxtail millet 40-day-old seedlings were subjected to different dehydration stress (42.0% RWC, 77.7% RWC) by transferring seedling roots into 1/3 hoagland nutrient solution containing 20% PEG6000 with bubbling. For 'forward' subtraction, RNA isolated from foxtail millet shoots and roots treated by dehydration stress was used as the 'tester'; for 'reverse' subtraction it was used as the 'driver'. Subtracted cDNA library was constructed using TA cloning kit

text: seedling

text: shoots and roots

text: Setaria italica

text: Foxtail millet reverse subtracted cDNA library in response to dehydration stress

text: pGEM T-easy(Promega)


text: China Agricultural University

text: live

text: Generic

text: Mar 51

text: seedling

text: shoots and roots

text: Setaria italica

text: Foxtail millet forward subtracted cDNA library in response to dehydration stress

characteristic: title

text: pGEM T-easy(Promega)


characteristic: External Id

text: Pastoral Genomics, c/o ViaLactia Biosciences (NZ) Ltd

text: live

text: Generic

text: Bronsyn

text: Etiolated seedlings

text: Lolium perenne

text: all tissues

text: Perennial ryegrass Etiolated seedlings

text: pBluescriptIISK+


text: live

text: Generic

text: Bronsyn

text: heat-stressed 8 hrs at 37 deg.

characteristic: description

text: seedlings (3 weeks)

text: Lolium perenne

text: leaf and stem

text: Perennial ryegrass heat-stressed 8 hrs

text: pBluescriptIISK+


characteristic: INSDC center name

text: live

text: Generic

text: Bronsyn

text: cold-acclimation.

text: seedlings (3 weeks)

text: Lolium perenne

text: leaf and stem

text: Perennial ryegrass cold-acclimation

text: pBluescriptIISK+


text: Instituto Butantan

text: live

text: Generic

text: 4ug of mRNA from Amblyomma cajennense tick salivary glands were primed with oligo-(dT) and reverse transcribed to cDNA using Superscript Plasmid System for cDNA Synthesis and Cloning (Invitrogen). The cDNAs were selected in agarose gel electrophoresis, linked to Eco RI adapters and directionally cloned in pGEM11Zf+ vector (Promega). ESTs were generated from random clones and grouped in unique sequences. The putative identification of each EST or cluster was obtained through Blast searches (e-value < e-05).

text: Salivary glands

text: Amblyomma cajennense

text: Eco RI

text: Not I

text: Salivary glands

text: Amblyomma cajennense tick library

text: plasmid


text: Instituto Butantan

text: live

text: Generic

text: 7ug of mRNA from Philodryas olfersii venom glands were primed with oligo-(dT) and reverse transcribed to cDNA using Superscript Plasmid System for cDNA Synthesis and Cloning (Invitrogen). The cDNAs were selected by size (350-600 pb and up 600 pb) in agarose gel electrophoresis, linked to Sal I adapters and directionally cloned in pSPORT-1 vector. ESTs were generated from random clones and grouped in clusters of unique sequences.

text: venom glands

text: Philodryas olfersii

text: Sal I

text: Not I

text: venom glands

text: Colubridae snake Philodryas olfersii cDNA library

text: Plasmid


text: University of Messina

text: live

text: Generic

text: pituitary gland

text: Xiphias gladius

text: cDNA RT-PCR Xiphias gladius pituitary gland


text: Universidade Federal de Minas Gerais

text: live

text: Generic

text: Secretory

characteristic: cell type

text: PCR-based cDNA library made with SMART cDNA library construction kit from Clontech

text: Adult

characteristic: dev-stage

text: Salivary gland

text: Triatoma brasiliensis

text: Male/Female

text: Ceara, Brazil

characteristic: strain

text: Salivary gland

text: Adult Triatoma brasiliensis salivary gland library TBSGBH-1

text: Phage

text: Lambda TriplEx2


text: Universidad Tecnica Federico Santa Maria

characteristic: INSDC first public

text: live

text: Generic

text: Carmenere

text: Fruit - bud - clusters

text: Vitis vinifera

text: Sal I

text: Not I

text: Mixture of buds, little clusters and fruits (C1)

characteristic: vector


characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Thompson-seedless

text: Inflorescence

text: Vitis vinifera

text: BsrG I

text: BsrG I

text: Inflorescence (VvS11)

text: pDONR


text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Thompson-seedless

text: Inflorescence

text: Vitis vinifera

text: BsrG I

text: BsrG I

text: Inflorescence with GA3 (VvS12)

text: pDONR


text: live

text: Generic

text: Carmenere

characteristic: lab-host

text: Bud - cluster

characteristic: organ

text: Vitis vinifera

text: BsrG I

text: BsrG I

text: Buds/little clusters (VvC2)

text: pDONR


text: live

text: Generic

text: Thompson-seedless

text: Fruit and Flower

text: Vitis vinifera

characteristic: organism

text: Sal I

text: Not I

text: Fruits and flowers treated with GA3 (VvS1)


text: University of Lausanne

text: live

text: Generic

text: The EST library was constructed from normalized cDNA that was prepared from total RNA using SMART technology and the duplex-specific nuclease normalization method (Evrogen). Total RNA was isolated from fire ants of both polygynous and monogynous colonies. All ants are from Georgia, USA. All clones are deposited with and publicly available at the BCCM/LMBP collection. (http://www.belspo.be/bccm/lmbp.htm)

text: Mixture of all stages: eggs, larvae, pupae and adults

text: Whole bodies

text: Solenopsis invicta

text: Mixture of queens, workers and males

characteristic: sex

text: Lausanne fire ant library

text: Plasmid

text: pAL16 (Evrogen)


text: National Institute of Allergy and Infectious Diseases

text: live

text: Generic

text: Secretory

text: Adult

text: Salivary gland

text: Argas monolakensis

text: Female

text: Salivary gland

characteristic: tissue-type

text: Adult Argas monolakensis salivary gland library AMSG2-Fed

characteristic: title

text: Phage

text: Lambda TriplEx2


characteristic: External Id

text: live

text: Generic

text: Secretory

characteristic: description

text: Adult

text: Salivary gland

text: Argas monolakensis

text: Female

text: Salivary gland

text: Adult Argas monolakensis salivary gland library AMSG1-Unfed

text: Phage

text: Lambda TriplEx2


text: IRD (Institut de Recherche pour le Developpement)

characteristic: INSDC center name

text: live

text: Generic

text: Library was built with ZAP Express lambda cDNA synthesis kit (STRATAGENE).

text: young leaves

text: Coffea canephora

text: EcoR I

text: Xho I

text: Coffea young leaves

text: phagemid

text: pBK-CMV Phagemid


text: live

text: Generic

text: Whole cherry

text: Coffea canephora

text: EcoR I

text: Xho I

text: Cherry of different development stage

text: phagemid

text: pBK-CMV Phagemid


text: Plant Biotechnology Institute, National Research Council Canada

text: live

text: Generic

text: Sequences have been trimmed to remove vector and low quality regions using LUCY sequence cleanup software (www.tigr.org).

text: 11 dap zygotic embryo

text: Brassica napus

text: Brassica napus EX 30-Lib 33

text: plasmid

text: pGemT-Easy


text: live

text: Generic

text: 26 dap zygotic embryo

text: Brassica napus

text: Brassica napus Ex 20-Lib10

text: plasmid

text: pGemT-Easy


text: live

text: Generic

text: 14 dap seed

text: Brassica napus

text: Brassica napus Ex 50 lib 7

text: plasmid


text: live

text: Generic

text: 11 dap zygotic embryo

text: Brassica napus

text: Brassica napus EX 30-Lib 32

text: plasmid

text: pGemT-Easy


text: live

text: Generic

text: 25 dap zygotic embryo

text: Brassica napus

text: Brassica napus Ex 50 lib 9

text: plasmid


text: live

text: Generic

text: 11 dap zygotic embryo

text: Brassica napus

text: Brassica napus EX 30-Lib 31

text: plasmid

text: pGemT-Easy


text: live

text: Generic

text: 14 dap seed

text: Brassica napus

text: Brassica napus Ex 50 lib 5

text: plasmid


text: live

text: Generic

text: 11 dap zygotic embryo

text: Brassica napus

text: Brassica napus EX 30-Lib 34

text: plasmid

text: pGemT-Easy


text: live

text: Generic

text: Secretory

characteristic: cell type

text: Adult

characteristic: dev-stage

text: Salivary gland

text: Anopheles funestus

text: Female

text: Salivary gland

text: Adult female Anopheles funestus salivary glands AFSG-MALI-1

text: Phage

text: Lambda TriplEx2


text: Institute of Microbiology, Chinese Academy of Sciences

characteristic: INSDC first public

text: live

text: Generic

text: Shandong ecotype

characteristic: cultivar

text: Total RNA was prepared from the whole Thellungiella plants using Trizol reagent (Gibico, USA). Total RNAs of different time points were mixed and used for further mRNA isolation according to manufacturer's protocol (Qiagen, Germany). Briefly, both first-strand cDNAs were synthesized and digested with Not I. Sal I adaptors were ligated to these digested cDNAs fragments, and then cDNAs of Thellungiella were directionally inserted into the pREP-5N vector.

text: 5-week-old seedlings

text: fission yeast

text: aerial part tissue

text: Eutrema halophilum

text: SalI

text: NotI

text: Thellungiella halophila fission yeast expression library


text: Melaka Institute of Biotechnology

characteristic: INSDC last update

text: live

text: Generic

text: Somatic

text: The cDNA library was prepared following all the guidelines and the protocols provided by manufacturer (Invitrogen Corporation) of the CloneMiner cDNA Library Construction Kit. This CloneMiner cDNA Library Construction Kit was used because it enables us to construct highly representative cDNA libraries without the risk of restriction enzymes cutting our precious cDNA clones /genes. Therefore, we can isolate fully intact clones from high-quality cDNA library.

text: 20 Days after anthesis (DAA)

text: Fruit (Pods)

text: Phaseolus vulgaris

text: BsrGI

text: BsrGI

text: Pod

text: Bean pod tissue cDNA Entry Library

text: Plasmid

text: Donor Vector, pDONR 222

characteristic: vector


text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Secretory

text: PCR-based cDNA library made with SMART cDNA library construction kit from Clontech

text: Adult

text: Salivary gland

text: Toxorhynchites amboinensis

text: Both

text: Salivary gland

text: Toxorhynchites amboinensis adult salivary gland cDNA library TASG-1

text: Phage

text: Lambda TriplEx2


text: Mount Desert Island Biological Laboratory

text: live

text: Generic

characteristic: NCBI submission package

text: The rectal gland was dissected from an adult spiny dogfish shark. Total RNA was extracted using TRIzol reagent followed by mRNA isolation. The primary cDNA library was directionally cloned in DH10B T1 and normalized by Invitrogen. The average insert size was 2.4 kb. Inserts were single-pass sequenced from the 5' end by Rexagen (now High-Throughput Sequencing Solutions, University of Washington, Seattle, WA). Traces were trimmed and prepared for submission to dbEST using trace2dbest software (Parkinson, Anthony and Blaxter, unpublished). This expressed sequence tag project was supported by NIH Grant number 1 R01 019732 from the National Center for Research Resources (D.W. Barnes, PI), the Woodcock Foundation (D.W. Barnes and C.J. Bayne, PI) and P20-RR-16463 from the INBRE Program of the National Center for Research Resources (P.H. Hand, PI).

text: rectal gland

text: Squalus acanthias

text: not determined

text: entire

text: Spiny dogfish shark rectal gland EST library


text: ViaLactia Biosciences (NZ) Ltd.

text: live

text: Generic

text: Poly-A mRNA was prepared from the brain of the fetus of a Friesian cow by oligo-dT cellulose affinity chromatography. The primary library was was constructed from poly-A-enriched RNA using the ZAP-cDNA Gigapack III Gold Kit (StrataGene) as intructed by the manufacturer, and normalized by an adaption of method 4 (Bonaldo et al., 1996) using in vitro-produced single-stranded circles as tracer.

text: 30-32 week fetus

text: Escherichia coli

characteristic: lab-host

text: Fetal brain

characteristic: organ

text: Bos taurus

text: EcoRI

text: XhoI

text: Male

text: Friesian

characteristic: strain

text: Fetal brain

text: FFB

text: Plasmid

text: pBluescript SK(+/-)


text: ViaLactia Biosciences (NZ) Ltd.

text: live

text: Generic

text: Poly-A mRNA was prepared from the mammary gland of a single pregnant lactating Friesian cow by oligo-dT cellulose affinity chromatography. The primary library was was constructed from poly-A-enriched RNA using the ZAP-cDNA Gigapack III Gold Kit (StrataGene) as intructed by the manufacturer, and normalized by an adaption of method 4 (Bonaldo et al., 1996) using in vitro-produced single-stranded circles as tracer.

text: Lactating

text: Escherichia coli

text: Mammary gland

text: Bos taurus

characteristic: organism

text: EcoRI

text: XhoI

text: Female

characteristic: sex

text: Friesian

text: Mammary gland

characteristic: tissue-type

text: FLM

text: Plasmid

text: pBluescript SK(+/-)


characteristic: External Id

text: ViaLactia Biosciences (NZ) Ltd.

text: live

text: Generic

text: Poly-A mRNA was prepared from the mammary glands of a single non-lactating, non-pregnant Friesian cow by oligo-dT cellulose affinity chromatography. The primary library was was constructed from poly-A-enriched RNA using the ZAP-cDNA Gigapack III Gold Kit (StrataGene) as intructed by the manufacturer, and normalized by an adaption of method 4 (Bonaldo et al., 1996) using in vitro-produced single-stranded circles as tracer.

characteristic: description

text: Adult, non-lactating, non-pregnant

text: Escherichia coli

text: Mammary gland

text: Bos taurus

text: EcoRI

text: XhoI

text: Female

text: Friesian

text: Mammary gland

text: FNM

characteristic: title

text: Plasmid

text: pBluescript SK(+/-)


text: New Mexico State University

characteristic: INSDC center name

text: live

text: Generic

text: adult

characteristic: dev-stage

text: accessory glands from 10 adult males

text: Allonemobius fasciatus

text: male

text: Accessory gland A. fasciatus library


text: Waksman Institute, Rutgers University

characteristic: INSDC first public

text: live

text: Generic

text: A. franciscana cDNAs were reversed transcribed from polyA-selected mRNAs. SfiIA and SfiIB linkers were added to the cDNAs via the primers used to synthesize first and second strand cDNAs. The cDNAs were cut with SfiI to generate SfiI sticky ends, and the cDNAs were directionally ligated into SfiIA and SfiIB-cut plasmid vector pTriplEx2. Recombinant plasmids were transformed into E. coli strain DH5a.

text: Mixed stages

text: E. coli DH5alpha

text: Whole animal

text: Artemia franciscana

text: EcoRI

text: Mixed male and female

text: WSSP A. franciscana cDNA Library SfiIA

text: Plasmid


text: University of Georgia

characteristic: INSDC last update

text: live

text: Generic

text: cDNA from Cronartium quercuum f. sp. fusiforme directly germinating basidiospores (from polystyrene surface) was hybridized to cDNA from Cronartium quercuum f. sp. fusiforme indirectly germinating basidiospores (from glass surface) in order to enrich for sequences expressed in directly germinating basidiospores.

text: germinating basidiospores

text: Cronartium quercuum f. sp. fusiforme

text: Directly germinating Cronartium quercuum f. sp. fusiforme basidiospores suppression subtraction hybridization libraries


text: University of Georgia

text: live

text: Generic

text: cDNA from Cronartium quercuum f. sp. fusiforme indirectly germinating basidiospores (from glass surface) was hybridized to cDNA from Cronartium quercuum f. sp. fusiforme directly germinating basidiospores (from polystyrene surface) in order to enrich for sequences expressed in indirectly germinating basidiospores.

text: germinating basidiospores

text: Cronartium quercuum f. sp. fusiforme

text: Indirectly germinating Cronartium quercuum f. sp. fusiforme basidiospores suppression subtraction hybridization libraries


text: University of Connecticut Health Center

text: live

text: Generic

text: adult

text: rabbit

text: salivary glands

text: Dermacentor andersoni

text: SfiIA

text: SfiIB

text: female

text: Tick SMART cDNA Library

text: phage

characteristic: vector


text: University of Victoria

text: live

characteristic: INSDC status

text: Generic

text: Tissue contributors: Robert Devlin (DFO, Vancouver, B.C.)

text: head kidney

text: Salmo salar

text: McConnell

text: ssalevf mixed_tissue Salmo salar cDNA


text: University of Victoria

text: live

text: Generic

characteristic: NCBI submission model

text: Tissue contributors: Vanya Ewart (IMB, Halifax, N.S.), Rachael Richie (RPC, Fredrickton, N.B.)

text: brain, kidney, spleen

text: Salmo salar

text: mixed tissue

text: ssalsjb mixed_tissue Salmo salar cDNA

text: pDNR-LiB


text: Auburn University

text: live

text: Generic

characteristic: NCBI submission package

text: Whole organism

text: Ichthyophthirius multifiliis

text: Sfi IA

text: Sfi IB

text: Whole organism normalized cDNA library

text: Phagemid

text: pDNR-LIB


text: Warsaw Agricultural University

text: live

text: Generic

text: RNA was isolated using Chomczynski method with Trizol reagent. 100 micrograms of RNA was used to amplification using primers designed to SERK sequence from GenBank. The RT-PCR product was cloned into the TOPO vector and transformed the recombinant vector into competent E. coli.

text: immature embryos

text: Secale cereale

text: Inbred line L318

text: Inbred line L318 TOPO TA Cloning

text: pUC plasmid

text: PCRII-TOPO


text: Cheju National University

text: live

text: Generic

text: Fuji

characteristic: cultivar

text: pathogen-infected plant

text: Fruit or Leaf

text: Malus domestica

text: Malus x domestica cv. Fuji


text: Chiangmai University

text: live

text: Generic

text: 14 days

text: intestines (jejunum)

characteristic: organ

text: Sus scrofa

text: male/female

text: Thai native pig

text: brush border

text: Piglet brush border cDNA clone


text: University of Nevada

text: live

text: Generic

text: Standard cDNA library construction was performed according to Agencourt's recommended protocol using the pAGEN-1 vector.

text: adult

characteristic: lab-host

text: microphyll fronds and roots

text: Selaginella lepidophylla

text: SLB

text: plasmid


text: Waksman Institute, Rutgers University

text: live

text: Generic

text: cDNAs were reversed transcribed from polyA-selected mRNA from A. franciscana. SfiIA and SfiIB linkers were added to the cDNAs via the primers used to synthesize first and second strand cDNAs respectively. The cDNAs were cut with SfiI to generate SfiI sticky ends, and the cDNAs were directionally ligated into SfiIA and SfiIB-cut plasmid vector pTriplEx2. Recombinant plasmids were transformed into E. coli strain DH5alpha.

text: Mixed stages

text: E. coli DH5alpha

text: Whole animal

text: Artemia franciscana

characteristic: organism

text: SfiI

text: Mixed male and female

text: WSSP A. franciscana cDNA library SfiI

text: Plasmid


text: National Institute of Allergy and Infectious Diseases

text: live

text: Generic

text: Secretory

characteristic: cell type

text: PCR-based cDNA library made with SMART cDNA library construction kit from Clontech

text: Fifth instar nymph

text: Salivary gland

text: Rhodnius prolixus

text: Salivary gland

text: Fifth instar salivary gland cDNA library RPSG-1

text: Phage

text: Lambda TriplEx2


text: live

text: Generic

text: Secretory

text: Adult

text: Salivary gland

text: Xenopsylla cheopis

text: Male/Female

characteristic: sex

text: Salivary gland

characteristic: tissue-type

text: Adult Xenopsylla cheopis salivary gland library XCSG-1

text: Phage

text: Lambda TriplEx2


characteristic: External Id

text: Children's National Medical Center

text: live

text: Generic

text: Muscle

text: Torpedo californica electric organ was grinded in liquid nitrogen and total RNA was isolated. Seven milligrams of total RNA was sent to Amplicon Express (Pullman, WA) for the construction of cDNA library (5.16 x 10^6 cfu/ml primary colonies). Cloning sites used for insertion were Eco RV (GGAATTC/GATATCAAG) and Not I (site of poly A tail [CCGCGGTGG/CGGCCGCT]). Colonies were gridded onto 384 well plates containing 15% glycerol. Clones were sequenced from the 5' end using the T3 primer (5'ATTAACCCTCACTAAAGGGA 3'). Clones were either sequenced by first plasmid purification or by direct PCR cloning.

characteristic: description

text: E. coli

text: Electric organ

text: Tetronarce californica

text: Electroplaque

text: Torpedo californica electric organ

text: pBluescript II KS(+)


text: BC Cancer Agency

characteristic: INSDC center name

text: live

text: Generic

text: 6 months old fetus

text: ElectroMAX DH10B T1 Phage-Resistant Cells

text: Fetal pancreas

text: Bos taurus

text: SalI (5' end of cDNA)

text: NotI (3' end of cDNA)

text: male

text: Hereford

characteristic: strain

text: Fetal pancreas

characteristic: title

text: pCMV SPORT 6.0


text: Center for Plant Molecular Biology

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Pericyle cells

text: 2.5 days after germination

characteristic: dev-stage

text: Primary root

text: Zea mays

text: Zea mays LCM amplified pericyle cell specific pGEM library

text: Plasmid

text: pGEM


text: Center for Plant Molecular Biology

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Non-pericyle central cylinder cells

text: 2.5 days after germination

text: Primary root

text: Zea mays

text: Zea mays LCM amplified non-pericyle central cylinder cell specific pGEM library

text: Plasmid

text: pGEM

characteristic: vector


text: Waksman Institute, Rutgers University

text: live

text: Generic

characteristic: NCBI submission package

text: A. franciscana cDNAs were reversed transcribed from polyA-selected mRNAs. EcoRI linkers were ligated to the cDNAs, which were subsequently ligated into the unique EcoRI site of plasmid vector pTriplEx2. Recombinant plasmids were transformed into E. coli strain DH5alpha.

text: Mixed stages

text: E. coli DH5alpha

text: Whole animal

characteristic: organ

text: Artemia franciscana

text: EcoRI

text: Mixed male and female

text: MBB A. franciscana cDNA EcoRI library

text: Plasmid


text: Waksman Institute, Rutgers University

text: live

text: Generic

text: Mixed stages

text: E. coli DH5alpha

characteristic: lab-host

text: Whole animal

text: Artemia franciscana

characteristic: organism

text: SfiI

text: Mixed male and female

text: MBB A. franciscana cDNA SfiI library

text: Plasmid


text: China Agricultural University

text: live

text: Generic

text: adult

text: Breast Muscle

text: Gallus gallus

text: Female

characteristic: sex

text: Silkie Fowl

text: Muscle

text: Chicken Breast Muscle cDNA Library


text: University of Bristol

text: live

text: Generic

text: pBluescript II XR ; Oligo(dT)-linker-primed, hemimethylated, 5_-EcoRI-cDNA-Xho1-3_PCR-based library. Gecarcoidea natalis cDNA PCR products of size >400 nucleotides containing EcoRI on the 5' end and Xho1 on the 3' end were directionally cloned into pBluescript II XR (Stratagene) following the cloning protocol. The cDNA insert can be excised by digestion with EcoRI and Xho1. The library was constructed by Dr. Ute Postel and Dr. Fiona Thompson at the University of Bristol (UK).

text: adult; intermoult

text: merus; penultimate leg

text: Gecarcoidea natalis

text: male

text: muscle

characteristic: tissue-type

text: G. natalis leg muscle expression library W (migrating 1)

text: Plasmid

text: pBluescript (r) II SK (+) XR vector


characteristic: External Id

text: University of Bristol

text: live

text: Generic

text: adult; intermoult

text: merus; penultimate leg

text: Gecarcoidea natalis

text: male

text: muscle

text: G. natalis leg muscle expression library D (dry season 2)

text: Plasmid

text: pBluescript (r) II SK (+) XR vector


text: Genome Institute of Singapore

text: live

text: Generic

text: Priming method: GsuI-d(T) priming ; Priming sequence: 5' -GAGCTAGTTCTGGAG(T)16VN -3' ; Directionally cloned: ; 5' cloning site: NotI site GCGGCCGC ; 5' linker/adaptor sequence: 5' -GGATCCGAC -3' ; 3' cloning site: BseRI ; Insert size range: 1.5kbp-700bp ; For PCR insert analysis: M13 forward and reverse primers ; Library is not amplified ; Recombinants (inserts): 98% ; Library complexity: 7.5 X 106 cfu ; Full-length construction (method): Ng et al, Nature Method 2005 2(2):105-111 ; The pooled tissue RNA was collected and used to construct full length enriched cDNA library and also served as template to synthesize complex first strand cDNA probe. Two high density colony arrays were made from over 110K cDNA clones and hybridized with the probes. Low abundant clones were selected by the Colony Array Subtractive Hybridization (CASH) analysis as they represented rare expressed clones. The hybridization intensities for all clones span from 0 to 300,000 counts and the low abundant selected clones in this library ranges from 0 to 13,000.

characteristic: description

text: Adult fish of 3-4 months old

text: Escherichia Top10

text: Gut/Internal Organs

text: Danio rerio

text: BseRI

text: Not I

text: Males and females mixed

text: Tubingen (TU)

text: Gut/Internal Organs

text: Plasmid


text: University of Maryland

characteristic: INSDC center name

text: live

text: Generic

text: cDNA was synthesized from total RNA isolated from total nerve cord tissue of juvenile Laupala kohalensis using Creator SMART system and then cloned into pDNR-lib after library normalization using Trimmer-Direct

text: juvenile at instars 5 to 8

text: total nerve cord

text: Laupala kohalensis

text: SfiI

text: SfiI

text: male and female

text: total nerve cord

text: plasmid

text: pDNR-lib from BD Clontech


text: University of Antwerp

text: live

text: Generic

text: gills kidney

text: Cyprinus carpio

text: Carp gill kidney cDNA normalization


text: live

text: Generic

text: Secretory

characteristic: cell type

text: Adult

text: Salivary gland

text: Oncopeltus fasciatus

text: Salivary gland

text: Adult Oncopeltus fasciatus salivary gland library AOFSG-1

text: Phage

text: Lambda TriplEx2


text: University of Victoria

text: live

text: Generic

text: Tissue contributors: Vanya Ewart (NRC Institute for Marine Biosciences, Halifax, N.S.)

text: brain, kidney, spleen

text: Osmerus mordax

text: mixed tissue

text: omorrgc mixed_tissue Osmerus mordax cDNA

characteristic: title


text: University of Victoria

characteristic: INSDC first public

text: live

text: Generic

text: Tissue contributors: Robert Devlin (DFO, Vancouver, B.C.)

text: brain, kidney, spleen

text: Oncorhynchus tshawytscha

text: mixed tissue

text: otshevc mixed_tissue Oncorhynchus tshawytscha cDNA


text: University of Victoria

characteristic: INSDC last update

text: live

text: Generic

text: brain, kidney, spleen

text: Oncorhynchus tshawytscha

text: mixed tissue

text: otshrgd mixed_tissue Oncorhynchus tshawytscha cDNA


text: University of Victoria

text: live

text: Generic

text: brain, kidney, spleen

text: Osmerus mordax

text: mixed tissue

text: omoreva mixed_tissue Osmerus mordax cDNA


text: UMR INRA PMS 1191

text: live

text: Generic

text: Parragio

characteristic: cultivar

text: A cDNA library of genes specifically expressed in embryo axis has been generated by a PCR-based suppressive subtractive hybridization (SSH) technique between mRNAs of two populations of embryo axis correspondent to two imbibition times 6 and 23 hours

text: germination

characteristic: dev-stage

text: embryo axis

text: Medicago truncatula

text: Medicago truncatula SSH 23 Hours

text: Plasmid

text: pGEMT


text: National Center for Cell Science

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: SMART cDNA and PCR-Select cDNA Subtraction kit from Clontech

text: Adult

text: Bacteria

text: Salivary Gland

text: Anopheles stephensi

text: Female

text: Mosquito salivary Gland SSH library

text: Plasmid

text: pGEMT


text: Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico

text: live

text: Generic

characteristic: NCBI submission package

text: Library constructed with total RNA from a single scorpion venom gland. The titer of the non-amplified library is 2.8x10^5 cfu/mL.

text: Adult

text: Venom gland

text: Hadrurus gertschi

text: Scorpion venom gland library 1

text: Phagemid

characteristic: vector


text: University of Queensland

text: live

text: Generic

text: A directionally cloned cDNA library was constructed from total RNA that was extracted from embryos, larvae and post-larvae of H. momus using a BD Biosciences SMART library construction kit. Phage were converted into plasmid DNA following manufacturer's instructions prior to standard plasmid DNA isolation and sequencing protocols.

text: embryos, larvae and post-larvae

characteristic: organ

text: Herdmania momus

text: SfiIA

text: SfiIB

text: Herdmania momus developmental microarray SMART cDNA library

text: Phage

text: lambda tripleX


text: UMR INRA/Universite Henri Poincare 1136

text: live

text: Generic

text: Douglas fir associated L. bicolor S238N sporocarps. The cDNA libray was constructed from 1 ug of total RNA using the SMART cDNA synthesis kit (Clontech, Palo Alto, CA, USA) according to the manufacturer's instructions. The resulting cDNA was packed into lambda phages using the Gigapack III Gold packaging kit (Stratagene, La Jolla, CA). The pTriplEx2 phagemid clones in Escherichia coli were obtained by using the mass in vivo excision protocol according to the manufacturer's instructions (Clontech).

text: 5 to 10 mm differentiating fruitbody

text: Fruitbody caps and stipes

text: Laccaria bicolor

characteristic: organism

text: Laccaria bicolor fruitbody library Lb2


text: UMR INRA/Universite Henri Poincare 1136

text: live

text: Generic

text: 30 to 40 mm mature fruitbody

text: Fruitbody caps

text: Laccaria bicolor

text: Laccaria bicolor fruitbody library Lb3


text: Colorado State University

text: live

text: Generic

text: Plant tissue was shipped in dry ice to Agencourt Bioscience Corporation (Beverly MA). Total RNA was extracted and optimized first strand cDNA synthesis was performed using a primer adapted with a rare enzyme cut site. cDNA fragments were size-selected by agarose gel electrophoresis, and directionally cloned into a pAGEN-1 vector. A positive control containing the TetR gene was used during construction of the primary library to ensure library quality. Single stranded DNA was made from a portion of the primary library by phagemid production, and reactions were treated with DNase I to ensure the removal of double stranded DNA. A second portion of the primary library was linearized and transcribed into anti-sense RNA with biotinylated dNTPs. Oligo dT and primer extension were used to pre-block the poly-A region prior to hybridization. The anti-sense RNA and single stranded circular DNA were hybridized, and abundant clones were removed using streptavidin. To reduce the amount of empty vectors, a Not1 oligo and Taq polymerase were used to synthesize double stranded DNA from the single stranded normalized library prior to final transfection. The normalized library was plated and 4969 clones were randomly selected for sequencing. Automated plasmid purification was achieved using the SPRI (SprintPrep) technique, which harvests plasmid DNA directly from lysed bacterial cultures, trapping both plasmid and genomic DNA to functionalized bead particles and selectively eluting only the plasmid (Beckman Biomek FX robots and CCS Packard DNATraks). DNA templates were sequenced in 384-well format using BigDye Version 3.1 reactions on ABI3730 instruments at Agencourt Biosciences. Thermal cycling was performed using 384-well Thermal cyclers (ABI, MJ Research). Sequencing Reactions were purified using Agencourt's CleanSeq dye-terminator removal kit.

text: Entire Plant (rosette and roots)

text: Centaurea maculosa

text: Centaurea normalized cDNA library

text: pAGEN (Agencourt Biosciences)

text: plasmid


text: Institute of Genetics and Developmental Biology, Chinese Academy of Sciences

text: live

text: Generic

text: Suppression subtractive hybridization library using Fusarium infected spikes as tester and mock inoculated spikes as driver

text: Spikes at anthesis

text: Hordeum vulgare

text: Fusarium induced barley EST SSH library