text: University of Wisconsin-Milwaukee

text: live

text: Generic

text: Library constructed from RNA obtained from the gills of adult quagga mussels exposed for 8 weeks at a site off the Milwaukee estuary, Lake Michigan.

text: Dreissena rostriformis bugensis

text: pooled gills

text: Milwaukee estuary quagga mussel gills

text: pBK-CMV


status: PUBLIC

characteristic: External Id

text: Institute of Hydrobiology, Chinese Academy of Sciences

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Pinctada imbricata

characteristic: organism

text: male

tag: attribute

characteristic: sex

text: testis

characteristic: tissue-type

text: gonad SMART cDNA library of Pinctada martensi

characteristic: title


text: MPI for Molecular Genetics

text: live

text: Generic

text: Non-normalized cDNA Library was constructed with polyA RNA from Epiperipatus sp.

characteristic: description

text: Epiperipatus sp. TB-2001

text: female

text: whole animal

text: adult Epiperipatus sp.

text: pBK-CMV

characteristic: vector


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 0.6-1.2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed and sequencing performed by DOE Joint Genome Institute (Walnut Creek, CA).

text: 1-cell stage through to juveniles

characteristic: dev-stage

text: DH10B T1R

characteristic: lab-host

text: Tubifex tubifex

text: SalI

text: NotI

text: Embryos and juveniles

text: CBAY Tubifex tubifex Embryos and juveniles (L)


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected >2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed and sequencing performed by DOE Joint Genome Institute (Walnut Creek, CA).

text: 1-cell stage through to juveniles

text: DH10B T1R

text: Tubifex tubifex

text: SalI

text: NotI

text: Embryos and juveniles

text: CBAZ Tubifex tubifex Embryos and juveniles (M)


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 1.2-2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed and sequencing performed by DOE Joint Genome Institute (Walnut Creek, CA).

text: 1-cell stage through to juveniles

text: Tubifex tubifex

text: SalI

text: NotI

text: Embryos and juveniles

text: CBFI Tubifex tubifex Embryos and juveniles


text: Instituto de Investigaciones Marinas (IIM), CSIC

text: live

text: Generic

text: Scophthalmus maximus

text: gill

text: Psetta maxima nodavirus-infected gill library


text: live

text: Generic

text: Scophthalmus maximus

text: kidney

text: Psetta maxima nodavirus-infected kidney library


text: live

text: Generic

text: Scophthalmus maximus

text: liver

text: Psetta maxima nodavirus-infected liver library


text: Sun Yat-sen University

text: live

text: Generic

text: Mikania micrantha

text: Mikania micrantha cDNA library


text: National Fisheries Research & Development Institute

text: live

text: Generic

text: gonad

characteristic: organ

text: Halocynthia roretzi

text: Halocynthia roretzi gonad cDNA library


text: live

text: Generic

text: liver

text: Halocynthia roretzi

text: Halocynthia roretzi liver cDNA library


text: National Institute for Plant Genome Research

text: live

text: Generic

characteristic: cultivar

text: cDNA library cloned into pGEM-T vector

text: Cicer arietinum

text: mature seed

text: chickpea seed cDNA library


characteristic: External Id

text: Universidad Nacional de La Plata

text: live

text: Generic

text: Negro Xamapa

text: sequences were obtained in a suppressive subtractive hibridization performed between RNA from root hairs of common bean inoculated with Rhizobium etli strain Santa Clara (tester) and root hairs of the same cultivar inoculated with the R. etli strain 55N1 (driver)

text: 7 day old seedlings

text: root

text: Phaseolus vulgaris

text: root hair

text: plasmid

text: pCR2.1 (pTOPO)


text: Stanford University

characteristic: INSDC center name

text: live

text: Generic

text: RNA from library 606 was filtered for common ESTs found in 606.

text: 0.5 cm to 2 cm

text: Stratagene XLOLR

text: Immature ear

text: Zea mays

text: EcoRI

text: XhoI

text: Inflorescence meristem - floral organ primordia

text: 953 - Immature ear with common ESTs screened by Schmidt lab

text: plasmid

text: ZAP Express (pBK-CMV)


text: Instituto Butantan

characteristic: INSDC first public

text: live

text: Generic

text: 5ug of mRNA from Bothrops insularis venom glands were primed with oligo-(dT) and reverse transcribed to cDNA using Superscript Plasmid System for cDNA Synthesis and Cloning (LifeTechnologies). The cDNAs were selected by size (350-600 pb and up 600 pb) in agarose gel electrophoresis, linked to SalI adapters and directionally cloned in pSPORT-1 vector (LifeTechnologies). ESTs were generated from random clones and grouped in unique sequences. The putative identification of each EST or cluster was obtained through Blast searches (e-value < e-05).

text: venom glands

text: Bothrops insularis

text: Sal I

text: Not I

text: venom glands

text: Snake Bothrops insularis library IL1

text: plasmid


text: Instituto Butantan

characteristic: INSDC last update

text: live

text: Generic

text: 5ug of mRNA from Bothrops insularis venom glands were primed with oligo-(dT) and reverse transcribed to cDNA using Superscript Plasmid System for cDNA Synthesis and Cloning (LifeTechnologies). The cDNAs were selected by size (350-600 pb and up 600 pb) in agarose gel electrophoresis, linked to Eco RI adapters and directionally cloned in pGEM11Zf+ vector (Promega). ESTs were generated from random clones and grouped in unique sequences. The putative identification of each EST or cluster was obtained through Blast searches (e-value < e-05).

text: venom glands

text: Bothrops insularis

text: Eco RI

text: Not I

text: venom glands

text: Snake Bothrops insularis library IL2

text: plasmid


text: Instituto Butantan

text: live

characteristic: INSDC status

text: Generic

text: venom glands

text: Bothrops insularis

text: Eco RI

text: Not I

text: venom glands

text: Snake Bothrops insularis library IL3

text: plasmid


text: Harvard Medical School

text: live

text: Generic

characteristic: NCBI submission model

text: mRNA was purified from zebrafish skeletal muscles. cDNA was synthesized using a XhoI-Oligo dT adaptor-primer. EcoRI adaptors were ligated, followed by digestion with XhoI, for directional cloning into pre-digested lambda ZAP Express vector.

text: adult

text: skeletal muscles

text: Danio rerio

text: EcoRI

text: XhoI

text: Zebrafish skeletal muscles cDNA library

text: Lambda ZAP Express


text: live

text: Generic

characteristic: NCBI submission package

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 2.2 kb. Library constructed by Life Technologies. Note: this is a NCI_CGAP Library.

text: DH10B (T1 phage-resistant)

text: ovary, PMSG-treated

text: Mus musculus

text: EcoRV

text: NotI

text: phagemid (ampicillin resistant)


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: RNA source pool of three ovaries, from females ranging in age from 38 to 49 yo. Library is oligo-dT primed and directionally cloned (EcoRV site is destroyed upon cloning). Average insert size 2.1 kb, insert size range 1-3.5 kb. Library is normalized and enriched for full-length clones and was constructed by C. Gruber (Invitrogen). Research Genetics tracking code 036.

text: ovary (pool of 3)

text: Homo sapiens

characteristic: organism

text: EcoRV (destroyed)

text: NotI

text: plasmid (ampicillin resistant)


text: Dept of AFNS, University of Alberta

text: live

text: Generic

text: Simple columnar epithelial

characteristic: cell type

text: Young adult

text: Intestine/duodenum

text: Bos taurus

text: EcoRI

text: Xho I

text: Smooth muscle

text: Bos taurus Duodenum #1 library


text: Dept of AFNS, University of Alberta

text: live

text: Generic

text: Simple columnar epithelial

text: Young adult

text: Intestine/jejunum

text: Bos taurus

text: EcoRI

text: Xho I

text: Smooth muscle

characteristic: tissue-type

text: Bos taurus Jejunum #1 library


text: Dept of AFNS, University of Alberta

text: live

text: Generic

text: Simple columnar epithelial

text: Young adult

text: Intestine/ileum

text: Bos taurus

text: EcoRI

text: Xho I

text: Smooth muscle

text: Bos taurus Ileum #1 library

characteristic: title


text: University of Colorado at Boulder

text: live

text: Generic

text: SL1-PCR-TOPO based library. Ancylostoma ceylanicum cDNA were PCR amplified, using SL1-EcoRI primer on the 5' end and oligo(dT)-XhoI on 3' end, were gel fractionated, and non-directionally cloned into pCR-TOPO-XL (Invitrogen), following TOPO-XL TA cloning protocol. The cDNA insert can be excissed with EcoRI. The library was constructed by Adriana Magalska and dr. Vadim Kapulkin at Department of Infectious Diseasaes, Microbiology and Immunology of Veterinary Faculty at the Warsaw Agricultural University. The Ancylostoma ceylanicum strain used in this work was originally provided by Dr. Jerzy Behnke at the University of Nottingham.

characteristic: description

text: adult, mixed sex

text: whole organism

text: Ancylostoma ceylanicum

text: EcoRI

text: EcoRI

text: female and male

characteristic: sex

text: whole organism

text: Vadim Kapulkin Ancylostoma ceylanicum adult SL1-PCR sub-library

text: pCR-TOPO-XL


text: University of Colorado at Boulder

text: live

text: Generic

text: infective larvae (L3)

characteristic: dev-stage

text: whole organism

text: Ancylostoma ceylanicum

text: EcoRI

text: EcoRI

text: female and male

text: whole organism

text: Vadim Kapulkin Ancylostoma ceylanicum infective larvae SL1-PCR sub-library

text: pCR-TOPO-XL

characteristic: vector


text: Texas A&M University

text: live

text: Generic

text: sequence 5' of the insert (5'-NNN...NNNinsert) GCGAATTGGAGCTCCACCGCGGTGGCGGCCGCGGCTCGAG. Sequence 3' of the inserts (AAGAATTCGATATCAAGCTTATCGATACCGTCGACCTCGAG. non-normalized library, sequenced 3' with M13R primer.

text: adipose tissue

characteristic: organ

text: Sus scrofa

text: NotI

text: EcoRI

text: adipose tissue

text: CSEQFXL35 adipose

text: Plasmid

text: pBluescript SK+


text: Texas A&M University

text: live

text: Generic

text: adrenal gland

text: Sus scrofa

text: NotI

text: EcoRI

text: CSEQFXL37 pig adrenal

text: Plasmid

text: pBluescript SK+


text: Texas A&M University

text: live

text: Generic

text: skeletal muscle

text: Sus scrofa

text: NotI

text: EcoRI

text: CSEQFXL34 pig skeletal muscle-rib eye

text: Plasmid

text: pBluescript SK+


text: Texas A&M University

text: live

text: Generic

text: thyroid and parathyroid gland

text: Sus scrofa

text: NotI

text: EcoRI

text: CSEQFXL38 pig thyroid and parathyroid

text: Plasmid

text: pBluescript SK+


text: University of Massachusetts Medical School

text: live

text: Generic

text: EST differential display fragment derived from cDNA population amplified by PCR from laser microdissected endometrial tissue section.

text: endometrium

text: Macaca mulatta

text: female

text: LCM PCR-amplified cDNA population


text: The University of Georgia, Department of Plant Biology

text: live

text: Generic

text: The library was made from poly-A RNA in the cloning vector lambda ZAPII. Clones to be sequenced were prepared by mass excision.

text: Mesenteric lymph node

text: Equus caballus

text: EcoRI

text: EcoRI

text: Mesenteric lymph node (MLN1)

text: Plasmid

text: pBluescript SK(-) from Lambda ZapII


text: live

text: Generic

text: Acute phase liver

text: Equus caballus

text: XhoI

text: EcoRI

text: Liver (APL1)

text: Plasmid

text: pBluescript SK(-) from Lambda ZapII


text: National Livestock Research Institute, RDA

text: live

text: Generic

text: myocyte

text: 24 months old

characteristic: lab-host

text: skeletal muscle

text: Bos taurus

text: EcoRI

text: Xho I

text: six males mixed

text: longissimus dorsi

text: Bos taurus LM-24-HW cDNA library

text: Uni-ZAPXR


characteristic: External Id

text: USDA/ARS/CCRU

text: live

text: Generic

text: Morex

characteristic: cultivar

text: Lemma and palea were collected from greenhouse grown Morex barley in the elongation stage of kernel development. Total RNA was isolated from the pooled lemma and palea. Poly(A)+ RNA was isolated using an oligo(dT) matrix. Suppression subtractive hybridization (SSH) was performed to create lemma/palea-enriched cDNA library using the PCR-Select cDNA subtraction kit (Clontech, Palo Alto, CA). The cDNA obtained from lemma/palea mRNA was used as a tester and cDNA from leaf mRNA served as a driver. After filling-in the ends, the entire population of lemma/palea-enriched cDNA was subjected to two rounds of PCR. The subtracted cDNA library was ligated to pCR2.1-TOPO TA cloning vector (Invitrogen, Carlsbad, CA) and introduced into TOP10F' competent cells.

text: Elongation kernel stage

text: Lemma and palea

text: Hordeum vulgare subsp. vulgare

text: RsaI

text: RsaI

text: Lemma/palea-enriched cDNA library from elongation stage of kernel


text: USDA/ARS/CCRU

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: Morex

text: Lemma and palea were collected from greenhouse grown Morex barley in the gelatinous stage of kernel development. Total RNA was isolated from the pooled lemma and palea. Poly(A)+ RNA was isolated using an oligo(dT) matrix. Suppression subtractive hybridization (SSH) was performed to create lemma/palea-enriched cDNA library using the PCR-Select cDNA subtraction kit (Clontech, Palo Alto, CA). The cDNA obtained from lemma/palea mRNA was used as a tester and cDNA from leaf mRNA served as a driver. After filling-in the ends, the entire population of lemma/palea-enriched cDNA was subjected to two rounds of PCR. The subtracted cDNA library was ligated to pCR2.1-TOPO TA cloning vector (Invitrogen, Carlsbad, CA) and introduced into TOP10F' competent cells.

text: Gelatinous kernel stage

text: Lemma and palea

text: Hordeum vulgare subsp. vulgare

text: RsaI

text: RsaI

text: Lemma/palea-enriched cDNA library from gelatinous stage of kernel


text: USDA/ARS/CCRU

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Morex

text: Lemma and palea were collected from greenhouse grown Morex barley in the dough stage of kernel development. Total RNA was isolated from the pooled lemma and palea. Poly(A)+ RNA was isolated using an oligo(dT) matrix. Suppression subtractive hybridization (SSH) was performed to create lemma/palea-enriched cDNA library using the PCR-Select cDNA subtraction kit (Clontech, Palo Alto, CA). The cDNA obtained from lemma/palea mRNA was used as a tester and cDNA from leaf mRNA served as a driver. After filling-in the ends, the entire population of lemma/palea-enriched cDNA was subjected to two rounds of PCR. The subtracted cDNA library was ligated to pCR2.1-TOPO TA cloning vector (Invitrogen, Carlsbad, CA) and introduced into TOP10F' competent cells.

text: Early dough kernel stage

text: Lemma and palea

text: Hordeum vulgare subsp. vulgare

text: RsaI

text: RsaI

text: Lemma/palea-enriched cDNA library from dough stage of kernel


text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: NCI_CGAP_Tr1 is a cDNA library containing the following tissue(s): Placenta Trophoblast. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is GGGTG.

text: DH10B (Life Technologies)

text: Trophoblast

text: Mus musculus

text: EcoR I

text: Not I

characteristic: strain

text: Trophoblast

text: Plasmid

text: pT7T3-Pac (Pharmacia) with a modified polylinker


text: National Cancer Institute / NIH

text: live

text: Generic

text: NCI_CGAP_EI1 is a normalized cDNA library containing the following tissue(s): Chondrosarcoma. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is ACACTTGCAC.

text: Adult

text: DH10B (Life Technologies)

text: Left Pelvis

text: Homo sapiens

characteristic: organism

text: EcoR I

text: Not I

text: Chondrosarcoma

characteristic: tissue-type

text: Plasmid


text: live

text: Generic

text: NCI_CGAP_ED0 is a cDNA library containing the following tissue(s): Chondrosarcoma cell line CS5. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is GCTCAAGGCT.

characteristic: description

text: Adult

text: DH10B (Life Technologies)

text: Left Pubic Bone

text: Homo sapiens

text: EcoR I

text: Not I

text: Chondrosarcoma

characteristic: title

text: Plasmid


text: live

text: Generic

text: NCI_CGAP_EI0 is a cDNA library containing the following tissue(s): Chondrosarcoma. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is ACACTTGCAC.

text: Adult

characteristic: dev-stage

text: DH10B (Life Technologies)

text: Left Pelvis

text: Homo sapiens

text: EcoR I

text: Not I

text: Chondrosarcoma

text: Plasmid


text: National Cancer Institute / NIH

text: live

text: Generic

text: NCI_CGAP_ED1 is a normalized cDNA library containing the following tissue(s): Chondrosarcoma cell line CS5. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is GCTCAAGGCT.

text: Adult

text: DH10B (Life Technologies)

text: Left Pubic Bone

text: Homo sapiens

text: EcoR I

text: Not I

text: Chondrosarcoma

text: Plasmid

characteristic: vector


text: Stanford University

text: live

text: Generic

text: RNA from library 606 was filtered for common ESTs found in 606.

text: 0.5 cm to 2 cm

text: Stratagene XLOLR

text: Immature ear

characteristic: organ

text: Zea mays

text: EcoRI

text: XhoI

text: Inflorescence meristem - floral organ primordia

text: 1091 - Immature ear with common ESTs screened by Schmidt lab

text: plasmid


text: Southern Illinois University at Carbondale

text: live

text: Generic

text: Total RNA was isolated from nuclear transferred and non-nuclear transferred fetal livers. cDNA amplification profiles generated by differential display of mRNA were compared. After visual comparison, differentially expressed fragments were purified and cloned in pGEM-T vector.

text: 45 days of gestation

text: Fetal liver

text: Bos taurus

text: Angus

text: Differentially expressed cDNAs in nuclear transferred fetal liver

text: Plasmid

text: pGEM


text: Auburn University

text: live

text: Generic

text: Head kidney

text: Ictalurus furcatus

text: NotI

text: SalI

text: Ictalurus furcatus head kidney cDNA library

text: Phagemid


text: National Cancer Institute / NIH

text: live

text: Generic

text: UI-R-DY1 is a normalized cDNA library containing the following tissue(s): Rat Cartilage from Femur and Tibia. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CTAATGGACG. The Rat cartilage tissue was provided by Dr Jeff Stevens at the University of Iowa.

text: ADULT

text: DH10B (Life Technologies)

characteristic: lab-host

text: Femur and Tibia

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Cartilage

text: Phagemid


characteristic: External Id

text: Bar-Ilan University

text: live

text: Generic

text: Erez and Elem female

text: young floral buds

text: Cucumis sativus

text: Cucumber floral bud cDNA library

text: lambda Zap II


text: Ludwig Institute for Cancer Research

text: live

text: Generic

text: A mini-library was made by cloning products derived from ORESTES PCR (U.S. Letters Patent application No. 196,716 - Ludwig Institute for Cancer Research) profiles into the pUC 18 vector. Reverse transcription of tissue mRNA and cDNA amplification were performed under low stringency conditions.

text: Adult

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: Bielefeld University

text: live

text: Generic

text: cDNA (lambda ZAP-II) library from sugar beet, whole plant mRNA, Prepared using the Stratagene UniZAP cDNA kit, cloning sites EcoRI-XhoI, primer sites and orientation: rev-T3-SacI-SK-EcoRI-GGCACGAGG-5pr-cDNA-polyA-XhoI-KpnI-T7-uni

text: 4 week old pot-grown plants

text: shoot and root

text: Beta vulgaris

text: Sugar beet MPIZ-ADIS-006 Lambda Zap II library

text: Plasmid

text: pBluescript SK- from lambda ZAP II


text: National Institute of Deafness and other Communication Disorders

text: live

text: Generic

text: The organ of Corti (OC) was fine dissected from a total of 386 OC as follows: 102 samples from post-natal (P) day 5; 72 from P6; 60 from P7; 46 from P8; 18 from P9; 20 from P10; 14 from P12 and 24 from P13. After killing animals by cervical dislocation followed by decapitation, the bulla was removed and opened in Leibowitz medium. The bony capsule of the cochlea was chipped away, stria vascularis and spiral ligament were removed and the sensory epithelium was carefully dissected out of the modiolus. Total RNA was extracted using the micro Fasttrack kit (catalog # K1593-02; Invitrogen, Carlsbad, CA), according to manufacturer's instructions. Reverse transcription and library construction were carried out with the Uni-Zap XR vector kit (catalog # 237211, Stratagene) and Uni-Zap XR Gigapack III Gold Cloning kit (catalog # 237612), both from Stratagene (La Jolla, CA, USA), according to manufacturer's instructions. Briefly: 1.5 ug mRNA was reverse transcribed using a hybrid oligo(dT) linker-primer that contains an Xho I site. First strand synthesis was primed with the linker- primer and transcribed using Moloney murine leukemia virus reverse transcriptase (MMLV-RT) and 5-methyl dCTP. The second strand was synthesized with DNA polymerase and RNase H. Complementary DNA was blunt ended with Pfu DNA polymerase, ligated with EcoR I adapters in the presence of ligase and digested with Xho I. The cDNA was sequentially size fractionated over Pharmacia Size Sep400 (Pharmacia, Uppsala, Sweden) and Clontech Chroma Spin-1000 (Clontech, Palo Alto, CA) columns to enrich for cDNAs greater than 400bp and 1000 bp, respectively. The cDNA was then directionally ligated to the Uni-ZAP XR vector, which had been predigested with EcoR I and Xho I. The phagemid was packaged with Gigapak III Gold and, upon titration on XL1 Blue MRF' cells, the yield of the phage library was estimated to be 11,100,000 recombinants. Stratagene's ExAssist Interference resistance helper phage (catalogue # 211203) was adopted to rescue plasmid DNA from the phages. Upon plating of the rescued library, individual cDNA clones were selected and grown in 96-well, 2 ml growth plate. Plasmid DNA was purified from 200 ul of saturated culture with the Concert96(TM) plasmid purification kit (Invitrogen, Carlsbad, CA) as instructed by the manufacturer. ESTs from the 5' end of the cDNA clones were generated with the universal M13 reverse primer (CAGGAAACAGCTATGACC) and 25% strength BigDye terminator sequencing chemistry (Applied Biosystems, Foster City, CA). Sequencing reactions were performed on MJ Tetrad thermal cyclers (MJ Research, Waltham, MA), and analyzed on 3700 automated capillary sequencers using POP5 polymer (Applied Biosystems, Foster City, CA). The frequency distribution of the library is as follows: 72% of genes have 1 copy; 14.3% 2; 12% 3-10; 1.4% 11-50 and 0.1% 51-150. As to gene function, 45% of genes are present in GenBank and have know function; 23% have hits in GenBank, but do not have assigned function; 12% are uncharacterized ESTs and 20% are unidentified.

text: Post natal day 5 to 13

text: Organ of Corti

text: Mus musculus

text: male and female

characteristic: sex

text: BALB/c

text: Mouse Organ of Corti cDNA pBluescript

text: Plasmid

text: pBluescript


text: live

text: Generic

text: NCI_CGAP_Ch1 is a cDNA library containing the following tissue(s): Chondrosarcoma Grade II. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is TGATCACGCT.

text: Adult

text: DH10B (Life Technologies)

text: Left Pelvis

text: Homo sapiens

text: EcoR I

text: Not I

text: Chondrosarcoma Grade II

text: Plasmid

text: pT7T3-Pac (Pharmacia) with a modified polylinker


text: live

text: Generic

text: NCI_CGAP_Ch2 is a normalized cDNA library containing the following tissue(s): Chondrosarcoma Grade II. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is TGATCACGCT.

text: Adult

text: DH10B (Life Technologies)

text: Left Pelvis

text: Homo sapiens

text: EcoR I

text: Not I

text: Chondrosarcoma Grade II

text: Plasmid


text: Crop and Food Research

characteristic: INSDC center name

text: live

text: Generic

text: Pukekohe Longkeeper

characteristic: cultivar

text: A. cepa seedlings were grown hydroponically (Randle et al., 1995) until bulbing. RNA was extracted from bulb meristem tissue and cDNA was prepared from polyA+ enriched RNA. The cDNA was directionally ligated into Uni-zap XR vector from Stratagene and packaged using Gigapack Gold packaging extracts. Plasmids containing cDNA inserts were mass-excised from phage stocks using ExAssist helper phage and propagated in SOLR cells. Clones were randomly chosen for sequencing.

text: bulbing

text: bulb meristem

text: Allium cepa

text: EcoRI

text: XhoI

text: onion bulb meristem library

text: Plasmid

text: pBluescript pSK


text: All India Institute of Medical Sciences

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: mRNA/cDNA differentially expressed between oral Squamous cell carcinoma and normal oral tissue

text: oral cavity (Buccal mucosa)

text: Homo sapiens

text: Male

text: Squamous cell carcinoma

text: Human oral squamous cell carcinoma mRNA

text: Plasmid

text: pGEM T-Easy


text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 2.2 kb. Constructed by ResGen, Invitrogen Corp. Note: this is a NIH_MGC Library.

text: DH10B (phage-resistant)

text: olfactory epithelium

text: Mus musculus

text: EcoRV

text: NotI

text: phagemid (ampicillin resistant)


text: Iowa State University

text: live

text: Generic

characteristic: NCBI submission package

text: Subtracted cDNA library of transcripts expressed in 3-day-old maize seedlings germinated from freeze-damaged seed constructed using suppression subtractive hybridization.

text: 3-Day-Old Seedling

text: Seedling Root

text: Zea mays

characteristic: strain

text: Maize Subtracted cDNA Library

text: Plasmid

text: pT-Adv (Clontech)


text: live

text: Generic

text: Anchor enzyme: NlaIII~ Normal spinal cord, derived from Clontech polyA+ RNA, catalog #6573-1, lot #0031205. Library constructed using the Microsage technique by D. Olschner in the laboratory of G. Riggins, M.D., Ph.D. (Duke University).

text: spinal cord

text: Homo sapiens

characteristic: organism

text: plasmid


text: University of Florida

text: live

text: Generic

text: Total RNA was isolated from selected parts of 70% ethanol-fixed larval midgut. cDNA was synthesized by SuperScript II reverse transcriptase with an oligo(dT) primers, TRsa or Cap; ligated to pseudo-double-stranded adaptor LUST-Rsa; and cloned into the pGEM-T vector (Matz, 2002).

characteristic: description

text: 4-th instar

text: anterior midgut

text: Aedes aegypti

text: transport epithelium

characteristic: tissue-type

text: anterior midgut #1 (AMG1)

text: pGEM-T


text: University of Florida

text: live

text: Generic

text: Total RNA was isolated from selected part of 70% ethanol-fixed larval midgut. cDNA was synthesized by SuperScript II reverse transcriptase with an oligo(dT) primers, TRsa or Cap; ligated to pseudo-double-stranded adaptor LUST-Rsa; and cloned into the pGEM-T vector (Matz, 2002).

text: 4-th instar larvae

characteristic: dev-stage

text: posterior midgut

text: Aedes aegypti

text: transport epithelium

text: posterior midgut #1 (PMG1)

characteristic: title

text: pGEM-T


text: University of Florida

text: live

text: Generic

text: 4-th instar larvae

text: Whole midgut

characteristic: organ

text: Aedes aegypti

text: transport epithelium

text: whole midgut #1 (WMG1)

text: pGEM-T

characteristic: vector


characteristic: External Id

text: Institute of Cancer Genetics, Columbia University

text: live

text: Generic

text: RT-PCR product was generated from Xenopus stage 20 RNA using the primers:-5' AGAGCCCAGAATGAAAATGC; 3' TGAAAGGAGAAAGCCATAGG

text: whole embryo

text: Xenopus laevis

text: Female

text: RT-PCR product from stage 20 RNA


text: Universite de Bordeaux I, Institut National de la Recherche Agronomique

text: live

text: Generic

text: Cabernet Sauvignon

text: Oriented library

text: veraison stage

text: Fruit without seeds

text: Vitis vinifera

text: SfiIA

text: SfiIB

text: Veraison Grape berries Lambda TriplEx2 Library

text: Phage

text: Lambda TriplEx2


text: live

text: Generic

text: Cabernet Sauvignon

text: Oriented library

text: Harvest - 15,5 weeks post-flowering

text: Fruit without seeds

text: Vitis vinifera

text: SfiIA

text: SfiIB

text: Red Grape berries Lambda TriplEx2 Library

text: Phage

text: Lambda TriplEx2


text: Princeton University

text: live

text: Generic

text: Stromal cell

characteristic: cell type

characteristic: cell-line

text: Two directionally cloned cDNA libraries were made from a hematopoietic stem cell-supporting stromal cell line (AFT024) and from a non-supporting stromal cell line (2018). Subtractive hybridization was performed by hybridization of the target, AFT024, single stranded cDNA library in pSport1 to biotinylated RNA transcribed from the driver, 2018 cDNA library in pSport2 with inserts cloned in the complementary orientation. The AFT024-subtracted library contains 4.2x105 clones and is depleted of common housekeeping gene products eg. beta-actin and enriched for transcripts specific to AFT024. For detailed protocols and additional information please see our website at http://stromalcell.princeton.edu.

text: Embryonic day 14-14.5

characteristic: lab-host

text: Fetal Liver

text: Mus musculus

text: Sal I

text: Not I

text: Fetal Liver

text: AFT024-subtracted library

text: Plasmid

text: Sport 1


text: Max-Planck-Institute for Plant Breeding Research

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: fragments were amplified from cDNA with degenerate primers and cloned into the vector

text: leaves or roots

text: Beta vulgaris

text: SH-RGA

text: Plasmid

text: pGEMT


text: University of Manchester Institute of Science and Technology (UMIST)

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: This normalized library was constructed from 1 million independent clones. cDNA synthesis was initiated using an oligo(dT) primer, using methylated C in the first strand synthesis reaction. Following this first strand reaction, double-stranded cDNA was blunted, ligated to NotI adapters, digested with EcoRI, size-selected, and cloned into the NotI and EcoRI compatible sites of a custom modified MCS of the pBluescript (KS+) vector. The library was normalized in 2 rounds using conditions adapted from Soares et al., PNAS (1994) 91: 9228-9232 and Bonaldo et al., Genome Research 6 (1996): 791, except that a significantly longer reannealing hybridization was used.

text: whole embryo

text: Gallus gallus

text: EcoRI

text: White Leghorn, Hisex

text: whole embryo

text: phagemid (ampicillin resisitant)

text: pBluescript II KS(+)


text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: adult

text: kidney + adrenal

text: Gallus gallus

text: EcoRI

text: Female

characteristic: sex

text: Compton Line 15I

text: phagemid (ampicillin resisitant)

text: pBluescript II KS(+)


text: live

text: Generic

characteristic: description

text: adult

text: small intestine

text: Gallus gallus

characteristic: organism

text: EcoRI

text: Female

text: Compton Line 15I

characteristic: strain

text: phagemid (ampicillin resisitant)

text: pBluescript II KS(+)


text: live

text: Generic

text: Library was constructed using 1.5 micrograms of polyA RNA (isolated from a pool of embryo tissues from White Leghorn, Hisex White breed chickens.) cDNA synthesis was initiated using an oligo(dT) primer, using methylated C in the first strand synthesis reaction. Following this first strand reaction, double-stranded cDNA was blunted, size-selected, and cloned into the NotI and EcoRI compatible sites of a custom modified MCS of the pcDNA 3.1 vector.

characteristic: dev-stage

text: whole embryo

text: Gallus gallus

text: EcoRI

text: White Leghorn, Hisex

text: whole embryo

characteristic: tissue-type

characteristic: title

text: phagemid (ampicillin resisitant)

text: pcDNA 3.1


characteristic: External Id

text: live

text: Generic

text: Modification of pBluescript II KS(+) [Stratagene] vector to accommodate cDNA produced with the T-trimmed protocol (Construction of uni-directionally cloned cDNA libraries from messenger RNA for improved 3' end DNA sequencing by Glenn Fu, et al. U.S. Patent # 6,387,624). Cut pBluescript II KS(+) with NotI and EcoRI. Ligate in double stranded adaptor containing BsgI and BamHI sites [5'ggccgcgtgcagccccggatccgaaaaaaag] [5'aattctttttttcggatccggggctgcacgc]

text: whole embryo

characteristic: organ

text: Gallus gallus

text: EcoRI

text: White Leghorn, Hisex

text: whole embryo

text: phagemid (ampicillin resisitant)

text: pBluescript II KS(+)

characteristic: vector


text: live

text: Generic

text: adult

characteristic: lab-host

text: kidney + adrenal

text: Gallus gallus

text: EcoRI

text: Female

text: Compton Line 15I

text: phagemid (ampicillin resisitant)

text: pBluescript II KS(+)


characteristic: INSDC center name

text: live

text: Generic

text: adult

text: small intestine

text: Gallus gallus

text: EcoRI

text: Female

text: Compton Line 15I

text: phagemid (ampicillin resisitant)

text: pBluescript II KS(+)


characteristic: INSDC first public

text: live

text: Generic

text: whole embryo

text: Gallus gallus

text: EcoRI

text: White Leghorn, Hisex

text: phagemid (ampicillin resisitant)

text: pBluescript II KS(+)


text: Institute of Biomedical Sciences, Academia Sinica

characteristic: INSDC last update

text: live

text: Generic

text: adult

text: brain, amygdala

text: Homo sapiens

text: Human Brain, amygdala


text: live

characteristic: INSDC status

text: Generic

text: NCI_CGAP_FG1 is a normalized cDNA library obtained from a pool of mRNA from 2 cell lines from Enchondroma tissues. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CGGTCACTC. The cell lines were provided by Dr. James Martin from the University of Iowa.

text: Adult

text: DH10B (Life Technologies)

text: Enchondroma

text: Homo sapiens

text: EcoR I

text: Not I

text: Cell lines

text: Plasmid

text: pT7T3-Pac (Pharmacia) with a modified polylinker


text: live

text: Generic

characteristic: NCBI submission model

text: NCI_CGAP_FE1 is a normalized cDNA library derived from a pool of mRNA obtained from 3 cell lines from grade II chondrosarcoma tissues. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CGCTACGGAC. The cell lines were provided by Dr James Martin from the University of Iowa.

text: Adult

text: DH10B (Life Technologies)

text: Chondrosarcoma

text: Homo sapiens

text: EcoR I

text: Not I

text: Cell lines

text: Plasmid


text: live

text: Generic

characteristic: NCBI submission package

text: NCI_CGAP_FL0 is a cDNA library derived from a pool of mRNA obtained from 4 cell lines from grade III chondrosarcoma tissues. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is GAGGTCGGTG. The cell line was provided by Dr James Martin from University of Iowa.

text: Adult

text: DH10B (Life Technologies)

text: Chondrosarcoma

text: Homo sapiens

text: EcoR I

text: Not I

text: Cell lines

text: Plasmid


text: live

text: Generic

text: NCI_CGAP_FL1 is a normalized cDNA library derived from a pool of mRNA obtained from 4 cell lines from grade III chondrosarcoma tissues. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is GAGGTCGGTG. The cell lines were provided by Dr. James Martin from the University of Iowa.

text: Adult

text: DH10B (Life Technologies)

text: Chondrosarcoma

text: Homo sapiens

text: EcoR I

text: Not I

text: Cell lines

text: Plasmid


text: National Cancer Institute / NIH

text: live

text: Generic

text: NCI_CGAP_FH1 is a normalized cDNA library obtained from a cell line derived from grade I chondrosarcoma tissue. The library was constructed and normalized according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is AGAATCCGGC. The cell line was provided by Dr. James Martin from the University of Iowa.

text: Adult

text: DH10B (Life Technologies)

text: Chondrosarcoma

text: Homo sapiens

text: EcoR I

text: Not I

text: Cell Line

text: Plasmid


text: Graduate School of Biotechnology

text: live

text: Generic

text: Jinhongmi

characteristic: cell-line

text: RNA was extracted from young storage root (0.3 - 1.0 cm in diameter) of sweetpotato. cDNA library was constructed with Uni-ZAP XR vector using ZAP cDNA synthesis kit (STRATAGENE, USA).

text: storage root

text: Ipomoea batatas

text: EcoRI

text: XhoI

text: hermaphrodite

characteristic: sex

text: Early developmental stage

text: pBluescript (SK)


text: Princeton University

text: live

text: Generic

text: Stem Cells; Lineageneg/lo, AA4.1pos, cKitpos, Ly6A/E (Sca-1)pos

characteristic: cell type

text: Two directionally cloned cDNA libraries were made from fetal liver hematopoietic stem cells enriched to be Lineageneg/lo, AA4.1pos, cKitpos, Ly6A/E (Sca-1)pos called Scapos and from AA4.1neg fetal liver cells. Subtractive hybridization was performed by hybridization of the target, Scapos, single stranded cDNA library in pSport1 to biotinylated RNA transcribed from the driver, AA4.1neg cDNA library in pSport2 with inserts cloned in the complementary orientation. For detailed protocols and additional information please see our website at http://stemcell.princeton.edu.

characteristic: description

text: Embryonic day 14-14.5

text: Fetal Liver

text: Mus musculus

characteristic: organism

text: Sal I

text: Not I

text: Hematopoietic

text: Hematopoietic Stem Cell Subtracted Library

text: Plasmid

text: Sport 1


text: Warsaw Agricultural University, Faculty of Horticulture, Biotechnology and Landscape Architecture

text: live

text: Generic

text: Borszczagowski

characteristic: cultivar

text: The cDNA clone was obtained after differential hybrydyzation between near isogenic line B10 (monoecious) and 2gg (gynoecious). The cDNA clone is specific for B10 male flower bud at the 1-2mm stage.

text: 1-2 mm

characteristic: dev-stage

text: 1-2 mm flower bud

text: Cucumis sativus

text: male

characteristic: strain

text: male flower bud cucumber cDNA DH B10/2gg Lambda ZAP Library

text: UNI-ZAPII XR vector


characteristic: External Id

text: Warsaw Agricultural University, Faculty of Horticulture and Landscape Architecture

text: live

text: Generic

text: 1-2 mm

text: 1-2 mm flower bud

characteristic: organ

text: Cucumis sativus

text: female (gynoecious)

text: female flower bud cucumber cDNA DH Gy3/Hgy3 Lambda ZAP Library

characteristic: title

text: UNI-ZAPII XR vector


text: Teagasc, Agriculture and Food Development Authority

characteristic: INSDC center name

text: live

text: Generic

text: A bovine corpus luteum cDNA library was constructed using the Lambda Zap Express/Gigapack cloning kit (Stratagene cloning systems). cDNA synthesis was carried out using an oligo-(dT) primer for the reverse transcription of 5(g of mRNA and the library was constructed by directional cloning EcoR1-Xho1 based on manufacturers instructions. An insert:vector ligation ratio of 1:5 was chosen as most optimum. The lambda library was packaged with Gigapack III gold packaging extracts and plated on the E. coli cell line XL1-Blue MRF'.

text: Day 6, 8 and 14 of the oestrus cycle

text: Corpus luteum

text: Bos taurus

text: Female

text: Corpus luteum

characteristic: tissue-type

text: Bovine Lambda Zap Express corpus luteum cDNA library

text: Phage

text: Lambda ZAP Express

characteristic: vector


text: Delaware Biotechnology Institute

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: cDNA names that include 'HR' are from the roots of hydroponically grown plants, names that include 'DS' are from developing seeds. All other clones are from two week old seedlings.

text: roots, developing seeds

text: Arabidopsis thaliana

text: Columbia

text: two week old seedlings

text: Size-selected small cDNAs of Arabidopsis thaliana


text: Philipps-Universitat Marburg

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Preparation of total RNA of pooled day 6 pc rabbit embryos from different mothers; first-strand synthesis with T11CA-primer; three PCRs with T11CA-primer and three different random-primers (A=TACAACGAGG; D=CTTTCTACCC; F=GGAACCAATC); separation of PCR-products by PAA-Gelelectrophoresis; cloning and sequencing of differential PCR-products.

text: day 6 post coitum

text: whole embryo

text: Oryctolagus cuniculus

text: Eco R I

text: Eco R I

text: undetermined

text: Weisser Neuseelander

text: differential display cDNAs of day 6 pc rabbit embryos

text: Plasmid

text: pCR II TOPO (Invitrogen)


text: Philipps-Universitat Marburg

text: live

text: Generic

text: Preparation of total RNA of pooled day 7 pc rabbit embryos from different mothers; first-strand synthesis with T11CA-primer; five PCRs with T11CA-primer and five different random-primers (A=TACAACGAGG; C=TGGATTGGTC; D=CTTTCTACCC; E=TTTTGGCTCC; F=GGAACCAATC); separation of PCR-products by PAA- Gelelectrophoresis; cloning and sequencing of differential PCR-products.

text: day 7 post coitum

text: whole embryo

text: Oryctolagus cuniculus

text: Eco R I

text: Eco R I

text: undetermined

characteristic: sex

text: Weisser Neuseelander

text: differential display cDNAs of day 7 pc rabbit embryos

text: Plasmid

text: pCR II TOPO (Invitrogen)


text: Philipps-Universitat Marburg

text: live

text: Generic

text: Preparation of total RNA of pooled day 8 pc rabbit embryos from different mothers; first-strand synthesis with T11CA-primer; two PCRs with T11CA-primer and two different random-primers (E=TTTTGGCTCC; F=GGAACCAATC); separation of PCR-products by PAA-Gelelectrophoresis; cloning and sequencing of differential PCR-products.

characteristic: description

text: day 8 post coitum

text: whole embryo

text: Oryctolagus cuniculus

text: Eco R I

text: Eco R I

text: undetermined

text: Weisser Neuseelander

text: differential display cDNAs of day 8 pc rabbit embryos

text: Plasmid

text: pCR II TOPO (Invitrogen)


text: Philipps-Universitat Marburg

text: live

text: Generic

text: Preparation of total RNA of pooled day 9 pc rabbit embryos from different mothers; first-strand synthesis with T11CA-primer; two PCRs with T11CA-primer and two different random-primers (D=CTTTCTACCC; F=GGAACCAATC); separation of PCR-products by PAA-Gelelectrophoresis; cloning and sequencing of differential PCR-products.

text: day 9 post coitum

text: whole embryo

text: Oryctolagus cuniculus

characteristic: organism

text: Eco R I

text: Eco R I

text: undetermined

text: Weisser Neuseelander

text: differential display cDNAs of day 9 pc rabbit embryos

text: Plasmid

text: pCR II TOPO (Invitrogen)


text: Stanford University

text: live

text: Generic

characteristic: cultivar

text: Schmidt lab dissected immature ears between 0.5 cm - 2.0 cm. Sharon Stanfield prepared the cDNA library in HybriZAP. Positive selection by probing with the pooled full-length cDNA of the following MADS box genes: ZAGL8B, ZAGL9B, ZAGL17, SI, ZAG1, ZAG2, ZAP1A, ZMM2, and ZPIA. Negative selection by probing with pooled 3'-end fragments of the same DNA. The final library is derived from a total of 210 selected plaques that hybridized with the full-length cDNA probes, but not with the 3'-end cDNA probes.

text: 0.5 cm - 2.0 cm

text: XLOLR

characteristic: lab-host

text: immature ear

text: Zea mays

text: EcoRI

text: XhoI

text: ear

text: 3528 - Positive selection of MADS-box genes from ear library 946

text: phagemid


text: University of Minnesota

text: live

text: Generic

text: ultra

text: Normal and proteoid roots of white lupin grown under P deficient conditions were harvested 7 and 10 DAE (days after emergence), and poly(A)+ RNA was isolated. The poly(A)+ RNA obtained from plants 7 and 10 DAE was combined in a 1:1 ratio and 7 ug total RNA was used for reconstruction of a proteoid root cDNA library in the phage ZAPII vector according to the manufacturer's instructions (Stratagene, La Jolla, CA, USA). For conversion of the phage library into the plasmid form, mass excision was performed according to the procedure described by Stratagene.

text: 7 and 10 DAE

characteristic: dev-stage

text: -P normal and proteoid root (cluster root)

text: Lupinus albus

text: EcoRI

text: XhoI

text: -P normal and proteoid roots 7 and 10 DAE

text: Plasmid

text: pBLUESCRIPT SK(+/-)


characteristic: External Id

text: University of Minnesota

text: live

text: Generic

text: ultra

text: Proteoid roots of white lupin grown under P deficient conditions were harvested 12 and 14 DAE (days after emergence), and poly(A)+ RNA was isolated. The poly(A)+ RNA obtained from plants 12 and 14 DAE was combined in a 1:1 ratio and 7 ug total RNA was used for reconstruction of a proteoid root cDNA library in the phage ZAPII vector according to the manufacturer's instructions (Stratagene, La Jolla, CA, USA). For conversion of the phage library into the plasmid form, mass excision was performed according to the procedure described by Stratagene.

text: 12 and 14 DAE

text: -P proteoid root (cluster root)

text: Lupinus albus

text: EcoRI

text: XhoI

text: -P proteoid roots 12 and 14 DAE

text: Plasmid

text: pBLUESCRIPT SK(+/-)


text: live

text: Generic

text: cDNA made by oligo-dT with attB2 site and directionally cloned. Priming sequence: 5'-TTTCCTGCAGGCCGGCCACCACTTTGTACAAGAAAGCTGGGTTTTTTTTTTTTTTTTTTT-3'. Full-length enriched library was constructed using the GeneRacer kit by Invitrogen, library amplification 16 cycles. Library constucted by Mark Bittinger in the Bradfield laboratory (McArdle Laboratory for Cancer Research, University of Wisconsin). Note: this is a NIH_MGC Library.

text: DH10B (phage-resistant)

text: Lung: TCDD (DMSO verhicle) Treated 48 Hours IP injections

characteristic: organ

text: Mus musculus

text: phagemid (Kanamycin resistant)


text: live

text: Generic

text: DH10B (phage-resistant)

text: Heart: TCCD (DMSO vehicle) treated 48 hours IP injection

text: Mus musculus

characteristic: title

text: phagemid (Kanamycin resistant)


text: live

text: Generic

text: DH10B (phage-resistant)

text: Kidney: TCDD (DMSO verhicle) Treated 48 Hours IP injections

text: Mus musculus

text: phagemid (Kanamycin resistant)


text: Shanghai Institute of Endocrinology, Ruijin Hospital, Shanghai Second Medical University

characteristic: INSDC center name

text: live

text: Generic

text: seven months

text: crural skeletal muscle

text: Rattus norvegicus

text: male

text: Sprague-Dawley

characteristic: strain

text: muscle tissue

text: Rat crural skeletal muscle cDNA library


text: UMR INRA/Universite Henri Poincare 1136

text: live

text: Generic

text: 'Beaupre'

text: cDNA library of two-month-old leaves from Populus trichocarpa Torr.& Gray x deltoides Bartr. Ex Marshall (clone 'Beaupre'). The cDNA library was constructed from 1 ug of total RNA using the SMART cDNA synthesis kit (Clontech, Palo Alto, CA, USA) according to the manufacturer's instructions. The resulting cDNA was packed into lambda phages using the Gigapack III Gold packaging kit (Stratagene, La Jolla, CA). The pTriplEx2 phagemid clones in Escherichia choli were obtained by using the mass in vivo excision protocol according to the manufacturer's instructions (Clontech).

text: two-month-old

text: developing leaf

text: Populus trichocarpa x Populus deltoides

text: two-month-old leaves from clone 'Beaupre'

characteristic: vector


text: University of California, Los Angeles

characteristic: INSDC first public

text: live

text: Generic

text: Hammond's Dwarf Scarlet

text: Embryo-proper regions were micro-dissected from globular-stage embryos six days after pollination from greenhouse-grown plants [Weterings et al., Plant Cell 13, 2409-2425 (2001)]. Double-stranded cDNA was synthesized from embryo-proper mRNA using the SMART cDNA Library Construction Kit according to the manufacturer (Clontech). The embryo-proper cDNA fragments were directionally ligated into the SfiI restriction site of the lambda TriplEx2 vector (Clontech), and the recombinant cDNAs were transformed into E. coli XL1-Blue cells (Clontech). Embryo-proper cDNA plasmids used for directional sequencing were obtained by in vivo excision from the lambda TriplEx2 recombinants in E. coli BM25.8 cells (Clontech).

text: 6-days post-pollination

text: Embryo-Proper Region of Globular-Stage Embryos

text: Phaseolus coccineus

text: SfiIA

text: SfiIB

text: Scarlet Runner Bean Embryo-Proper Region

text: Plasmid


text: University of California, Los Angeles

characteristic: INSDC last update

text: live

text: Generic

text: Hammond's Dwarf Scarlet

text: Suspensor regions were micro-dissected from globular-stage embryos six days after pollination from greenhouse-grown plants [Weterings et al., Plant Cell 13, 2409-2425 (2001)]. Double-stranded cDNA was synthesized from suspensor mRNA using the SMART cDNA Library Construction Kit according to the manufacturer (Clontech). The suspensor cDNA fragments were directionally ligated into the SfiI restriction site of the lambda TriplEx2 vector (Clontech), and the recombinant cDNAs were transformed into E. coli XL1-Blue cells (Clontech). Suspensor cDNA plasmids used for directional sequencing were obtained by in vivo excision from the lambda TriplEx2 recombinants in E. coli BM25.8 cells (Clontech).

text: 6-days post-pollination

text: Suspensor Region of Globular-Stage Embryos

text: Phaseolus coccineus

text: SfiIA

text: SfiIB

text: Scarlet Runner Bean Suspensor Region TriplEx2

text: Plasmid


text: University of California, Los Angeles

text: live

characteristic: INSDC status

text: Generic

text: Hammond's Dwarf Scarlet

text: Suspensor regions were micro-dissected from globular-stage embryos six days after pollination from greenhouse-grown plants [Weterings et al., Plant Cell 13, 2409-2425 (2001)]. Double-stranded cDNA was synthesized from suspensor mRNA using the SMART cDNA Library Construction Kit according to the manufacturer (Clontech). The suspensor cDNA fragments were ligated non-directionally into the pCR2.1 vector {Invitrogen), and the recombinant cDNAs were transformed into E. coli INV alpha F' cells (Invitrogen).

text: 6-days post-pollination

text: Phaseolus coccineus

text: EcoRI

text: EcoRI

text: Scarlet Runner Bean Suspensor Region pCR2.1

text: Plasmid


text: Animal and Natural Resources Institute

text: live

text: Generic

characteristic: NCBI submission model

text: Cot500 Normalized library, library constructed from equimolar mRNA pools derived from eight different mammary tissue types, These clones consist of ESTs that were unique against all databases. prepubertal fat,prepubertal epithelium, midgestation, involution, cholostrogenesis

text: Multiple

text: Mammary gland

text: Bos taurus

text: Notl

text: SaII

text: Female

text: Holstein

text: Pooled

characteristic: tissue-type

text: BARC 5BOV BtGI Unique

text: plasmid

text: pCMV SPORT6


text: Southwest Agricultural University

text: live

text: Generic

characteristic: NCBI submission package

text: embryonic day 1

text: unfertilized eggs

text: Bombyx mori

text: EcoR I

text: female

characteristic: sex

text: unfertilized eggs

text: Bombyx mori unfertilized egg cDNA library

text: Phage

text: lambda ExCell


text: UMR INRA/Universite Henri Poincare 1136

text: live

text: Generic

text: Eucalyptus globulus ssp. bicostata Kirkp.; cDNA library from ectomycorrhizas of Pisolithus microcarpus/Eucalyptus globulus harvested after four days of contact between the symbiotic partners. Ectomycorrhizas were synthesized in vitro applying the vertically oriented petri-dish system. The cDNA library was constructed from 1 ug of total RNA using the SMART cDNA synthesis kit (Clontech, Palo Alto, CA, USA) according to the manufacturer's instructions. The resulting cDNA was packed into lambda phages using the Gigapack III Gold packaging kit (Stratagene, La Jolla, CA). The pTriplEx2 phagemid clones in Escherichia choli were obtained by using the mass in vivo excision protocol according to the manufacturer's instructions (Clontech).

characteristic: description

text: four-days-old

text: ectomycorrhiza

text: Eucalyptus globulus subsp. bicostata

characteristic: organism

text: Eucalyptus globulus cDNA after four days contact between symbiotic partners Pisolithus microcarpus/Eucalyptus globulus


text: UMR INRA/Universite Henri Poincare 1136

text: live

text: Generic

text: Eucalyptus globulus ssp. bicostata Kirkp.; cDNA library from ectomycorrhizas of Pisolithus microcarpus/Eucalyptus globulus harvested after 12 days of contact between the symbiotic partners. Ectomycorrhizas were synthesized in vitro applying the vertically oriented petri-dish system. The cDNA library was constructed from 1 ug of total RNA using the SMART cDNA synthesis kit (Clontech, Palo Alto, CA, USA) according to the manufacturer's instructions. The resulting cDNA was packed into lambda phages using the Gigapack III Gold packaging kit (Stratagene, La Jolla, CA). The pTriplEx2 phagemid clones in Escherichia choli were obtained by using the mass in vivo excision protocol according to the manufacturer's instructions (Clontech).

text: ectomycorrhiza

text: Eucalyptus globulus subsp. bicostata

text: Eucalyptus globulus cDNA after 12 days contact between symbiotic partners Pisolithus microcarpus/Eucalyptus globulus


text: UMR INRA/Universite Henri Poincare 1136

text: live

text: Generic

characteristic: cultivar

text: Pisolithus microcarpus Coker & Mass. Cunn. nom prov.; cDNA library from ectomycorrhizas of Pisolithus microcarpus/Eucalyptus globulus harvested after four days of contact between the symbiotic partners. Ectomycorrhizas were synthesized in vitro applying the vertically oriented petri-dish system. The cDNA library was constructed from 1 ug of total RNA using the SMART cDNA synthesis kit (Clontech, Palo Alto, CA, USA) according to the manufacturer's instructions. The resulting cDNA was packed into lambda phages using the Gigapack III Gold packaging kit (Stratagene, La Jolla, CA). The pTriplEx2 phagemid clones in Escherichia choli were obtained by using the mass in vivo excision protocol according to the manufacturer's instructions (Clontech).

text: four-days-old

text: ectomycorrhiza

text: Pisolithus microcarpus

text: Pisolithus microcarpus cDNA after four days contact between symbiotic partners Pisolithus microcarpus/Eucalyptus globulus


text: UMR INRA/Universite Henri Poincare 1136

text: live

text: Generic

text: Eucalyptus globulus ssp. bicostata Kirkp.; cDNA library from ectomycorrhizas of Pisolithus microcarpus/Eucalyptus globulus harvested after 21 days of contact between the symbiotic partners. Ectomycorrhizas were synthesized in vitro applying the vertically oriented petri-dish system. The cDNA library was constructed from 1 ug of total RNA using the SMART cDNA synthesis kit (Clontech, Palo Alto, CA, USA) according to the manufacturer's instructions. The resulting cDNA was packed into lambda phages using the Gigapack III Gold packaging kit (Stratagene, La Jolla, CA). The pTriplEx2 phagemid clones in Escherichia choli were obtained by using the mass in vivo excision protocol according to the manufacturer's instructions (Clontech).

characteristic: dev-stage

text: ectomycorrhiza

text: Eucalyptus globulus subsp. bicostata

text: Eucalyptus globulus cDNA after 21 days contact between symbiotic partners Pisolithus microcarpus/Eucalyptus globulus


characteristic: External Id

text: UMR INRA/Universite Henri Poincare 1136

text: live

text: Generic

text: Pisolithus microcarpus Coker & Mass. Cunn. nom prov.; cDNA library from ectomycorrhizas of Pisolithus microcarpus/Eucalyptus globulus harvested after 12 days of contact between the symbiotic partners. Ectomycorrhizas were synthesized in vitro applying the vertically oriented petri-dish system. The cDNA library was constructed from 1 ug of total RNA using the SMART cDNA synthesis kit (Clontech, Palo Alto, CA, USA) according to the manufacturer's instructions. The resulting cDNA was packed into lambda phages using the Gigapack III Gold packaging kit (Stratagene, La Jolla, CA). The pTriplEx2 phagemid clones in Escherichia choli were obtained by using the mass in vivo excision protocol according to the manufacturer's instructions (Clontech).

text: ectomycorrhiza

text: Pisolithus microcarpus

text: Pisolithus microcarpus cDNA after 12 days contact between symbiotic partners Pisolithus microcarpus/Eucalyptus globulus


text: UMR INRA/Universite Henri Poincare 1136

text: live

text: Generic

text: Pisolithus microcarpus Coker & Mass. Cunn. nom prov.; cDNA library from ectomycorrhizas of Pisolithus microcarpus/Eucalyptus globulus harvested after 21 days of contact between the symbiotic partners. Ectomycorrhizas were synthesized in vitro applying the vertically oriented petri-dish system. The cDNA library was constructed from 1 ug of total RNA using the SMART cDNA synthesis kit (Clontech, Palo Alto, CA, USA) according to the manufacturer's instructions. The resulting cDNA was packed into lambda phages using the Gigapack III Gold packaging kit (Stratagene, La Jolla, CA). The pTriplEx2 phagemid clones in Escherichia choli were obtained by using the mass in vivo excision protocol according to the manufacturer's instructions (Clontech).

text: ectomycorrhiza

characteristic: organ

text: Pisolithus microcarpus

text: Pisolithus microcarpus cDNA after 21 days contact between symbiotic partners Pisolithus microcarpus/Eucalyptus globulus


text: UMR INRA/Universite Henri Poincare 1136

text: live

text: Generic

text: Laccaria bicolor Maire Orton; cDNA library from mycelium of Laccaria bicolor grown for three weeks on agar-plates containing high sugar (20 g/l glucose, 5 g/l maltose) Pachlewski medium. The cDNA library was constructed from 1 ug of total RNA using the SMART cDNA synthesis kit (Clontech, Palo Alto, CA, USA) according to the manufacturer's instructions. The resulting cDNA was packed into lambda phages using the Gigapack III Gold packaging kit (Stratagene, La Jolla, CA). The pTriplEx2 phagemid clones in Escherichia choli were obtained by using the mass in vivo excision protocol according to the manufacturer's instructions (Clontech).

text: three-weeks-old

text: free-living vegetative mycelium

text: Laccaria bicolor

characteristic: title


text: Cold Spring Harbor Laboratory

characteristic: INSDC center name

text: live

text: Generic

text: NYBG acc# 76163D

text: Date: Complete 07/03/2002, submitted to CSHL 07/09/2002 Library: Stratagene ZAP Express cDNA Synthesis Kit. The library was size-fractionated to enrich for large inserts.

text: Megeasporophyll

text: Ginkgo biloba

text: Xho I

text: Eco RI

text: Female

text: Gingko megasporophyll (NYBG)

text: Phage

text: pBK-CMV


text: Cold Spring Harbor Laboratory

text: live

text: Generic

text: Date: Completed 02/11/02. Submitted to CSHL 02/12/02 Library: Stratagene ZAP Express cDNA Synthesis Kit. The library was size-fractionated to enrich for large inserts. Sample: NYBG accession number #436/84

text: young leaves

text: Gnetum gnemon

text: XhoI

text: Eco RI

text: female

text: Gnetum leaves (NYBG)

text: phage

text: pBK-CMV


text: Cold Spring Harbor Laboratory

text: live

text: Generic

text: Date: Completed 08/09/2002. Submitted to CSHL 08/21/2002. Sample: Young emergent sporopyhll with ovules detached (ovules used to make a separate library). From New York Botanical Garden Conservatory accssion number 808/59 A. (collected 06/04/2002). Library: Made using Stratagene's ZAP Express Vector Kit. Library was size fractionated for large inserts.

text: Sporophyll with ovules detached.

text: Cycas rumphii

text: Xho I

text: Eco RI

text: Female

text: Cycas sporophyll (w/o ovule) (NYBG)

text: Phage

text: PBK-CMV


text: Cold Spring Harbor Laboratory

text: live

text: Generic

text: NYBG acc# 76163B

text: Date: Complete 7/3/02, sent to CSHL 7/9/02. Library: Stratagene ZAP Express cDNA Synthesis Kit. The library was size-fractionated to enrich for large inserts.

text: Microsporophyll

text: Ginkgo biloba

text: Xho I

text: Eco RI

text: Male

text: Gingko microsporophyll (NYBG)

text: Phage

text: pBK-CMV


text: Cold Spring Harbor Laboratory

text: live

text: Generic

text: Date: Completed 02/11/02, submitted for sequencing 02/12/02. Library: Stratagene ZAP Express cDNA Synthesis Kit. The library was size-fractionated to enrich for large inserts. Sample: NYBG accession number #436/84

text: mature, unfertilized reproductive strobili

text: Gnetum gnemon

text: XhoI

text: Eco RI

text: female

text: Gnetum female cone (NYBG)

text: phage

text: pBK-CMV


text: Cold Spring Harbor Laboratory

text: live

text: Generic

text: Date: Completed 08/09/2002. Submitted to CSHL 08/21/2002. Sample: Young emergent cones. From Montgomery Botanical Center accession #99798D (collected 04/25/2002). Library: Made using Stratagene's ZAP Express Vector Kit. Library was size fractionated for large inserts.

text: Immature cone

text: Zamia furfuracea

text: Xho I

text: Eco RI

text: Male

text: Zamia immature male cone (NYBG)

text: PHAGE

text: pBK-CMV


text: University of Perugia

text: live

text: Generic

text: B17 x P13 F1 population

text: This EST was obtained with the cDNA-AFLP method (Bachem et al.Plant Journal 9:745-753, 1996). Total RNA was extracted from three female fertile and thr ee female sterile alfalfa plants from an F1 population obtained by crossing th e plants B17 and P13 (Rosellini et al. Theoretical and Applied Genetics, 97:1 289- 1295, 1998). Equal amounts of RNA from each fertile or sterile plant were b ulked and used for the cDNA-AFLP protocol. The restriction endonucleases EcoRI an d MseI were used. Bands that were polymorphic between the female sterile and female fertile bulks were isolated from the polyacrilamide gel, cloned in a plasmid vector (Invitrogen PCR4 TOPO) and sequenced using the M13F primer. The published sequence begins and ends with the EcoRI (GAATTC) and MseI (TTAA) recognition sequences, respectively. The technical help of Francesco Panara is gratefully acknowledged.

text: From ovule primordia to anthesis

text: E.coli

characteristic: lab-host

text: Developing flower buds

text: Medicago sativa

text: hermaphrodite

text: Alfalfa developing flower bud library

text: Plasmid

text: Invitrogen PCR4 TOPO Vector


text: Texas A&M University

text: live

text: Generic

text: sequence 5' of the insert (5'-NNN...NNNinsert) GCGAATTGGAGCTCCACCGCGGTGGCGGCCGCGGCTCGAG. Sequence 3' of the inserts (AAGAATTCGATATCAAGCTTATCGATACCGTCGACCTCGAG. non-normalized library, sequenced 3' with M13R primer.

text: hypothalamus

text: Bos taurus

text: NotI

text: EcoRI

text: hypothalamus

text: CSEQFXL18 hypothalamus

text: Plasmid

text: pBluescript SK+

characteristic: vector


text: Texas A&M University

characteristic: INSDC first public

text: live

text: Generic

text: sequence 5' of the insert (5'-NNN...NNNinsert) GCGAATTGGAGCTCCACCGCGGTGGCGGCCGCGGCTCGAG. Sequence 3' of the inserts (AAGAATTCGATATCAAGCTTATCGATACCGTCGACCTCGAG. normalized Rd 2 library, sequenced 3' with M13R primer.

text: hypothalamus

text: Bos taurus

text: NotI

text: EcoRI

text: hypothalamus

text: CSEQFXN27 hypothalamus

text: Plasmid

text: pBluescript SK+


text: Texas A&M University

characteristic: INSDC last update

text: live

text: Generic

text: sequence 5' of the insert (5'-NNN...NNNinsert) GCGAATTGGAGCTCCACCGCGGTGGCGGCCGCGGCTCGAG. Sequence 3' of the inserts (AAGAATTCGATATCAAGCTTATCGATACCGTCGACCTCGAG. normalized Rd 1 library, sequenced 3' with M13R primer.

text: hypothalamus

text: Bos taurus

text: NotI

text: EcoRI

text: hypothalamus

text: CSEQFXN32 hypothalamus

text: Plasmid

text: pBluescript SK+


text: Texas A&M University

text: live

characteristic: INSDC status

text: Generic

text: adipose tissue

text: Bos taurus

text: NotI

text: EcoRI

text: adipose tissue

text: CSEQFXL19 adipose

text: Plasmid

text: pBluescript SK+


text: Texas A&M University

text: live

text: Generic

characteristic: NCBI submission model

text: muscle, rib eye

text: Bos taurus

text: NotI

text: EcoRI

text: muscle, rib eye

text: CSEQFXN16 muscle, rib eye

text: Plasmid

text: pBluescript SK+


text: Texas A&M University

text: live

text: Generic

text: small intestine

text: Bos taurus

text: NotI

text: EcoRI

text: small intestine

characteristic: tissue-type

text: CSEQFXL04 small intestine

text: Plasmid

text: pBluescript SK+


text: Texas A&M University

text: live

text: Generic

characteristic: NCBI submission package

text: muscle, rib eye

text: Bos taurus

text: NotI

text: EcoRI

text: muscle, rib eye

text: CSEQFXL25 muscle, rib eye

text: Plasmid

text: pBluescript SK+


text: Texas A&M University

text: live

text: Generic

text: muscle, rib eye

text: Bos taurus

text: NotI

text: EcoRI

text: muscle, rib eye

text: CSEQFXN17 muscle, rib eye

text: Plasmid

text: pBluescript SK+


text: Texas A&M University

text: live

text: Generic

text: sequence 5' of the insert (5'-NNN...NNNinsert) GCGAATTGGAGCTCCACCGCGGTGGCGGCCGCGGCTCGAG. Sequence 3' of the inserts (AAGAATTCGATATCAAGCTTATCGATACCGTCGACCTCGAG. normalized RD 1 library, sequenced 3' with M13R primer.

text: small intestine

text: Bos taurus

text: NotI

text: EcoRI

text: small intestine

text: CSEQFXN47 small intestine

text: Plasmid

text: pBluescript SK+


text: Texas A&M University

text: live

text: Generic

text: stomach-rumen

text: Bos taurus

text: NotI

text: EcoRI

text: stomach-rumen

text: CSEQFXL05 stomach-rumen

text: Plasmid

text: pBluescript SK+


text: Texas A&M University

text: live

text: Generic

text: sequence 5' of the insert (5'-NNN...NNNinsert) GCGAATTGGAGCTCCACCGCGGTGGCGGCCGCGGCTCGAG. Sequence 3' of the inserts (AAGAATTCGATATCAAGCTTATCGATACCGTCGACCTCGAG. normalized RD 2 library, sequenced 3' with M13R primer.

text: small intestine

text: Bos taurus

text: NotI

text: EcoRI

text: small intestine

text: CSEQFXN48 small intestine

text: Plasmid

text: pBluescript SK+


text: Texas A&M University

text: live

text: Generic

text: stomach-reticulum

text: Bos taurus

text: NotI

text: EcoRI

text: stomach-reticulum

text: CSEQFXL06 stomach-reticulum

text: Plasmid

text: pBluescript SK+


text: Texas A&M University

text: live

text: Generic

text: stomach-omasum

text: Bos taurus

text: NotI

text: EcoRI

text: stomach-omasum

text: CSEQFXL22 stomach-omasum

text: Plasmid

text: pBluescript SK+


text: Texas A&M University

text: live

text: Generic

text: stomach-abomasum

text: Bos taurus

text: NotI

text: EcoRI

text: stomach-abomasum

text: CSEQFXL23 stomach-abomasum

text: Plasmid

text: pBluescript SK+


text: Texas A&M University

text: live

text: Generic

text: stomach-abomasum

text: Bos taurus

text: NotI

text: EcoRI

text: stomach-abomasum

text: CSEQFXL06 stomach-abomasum

text: Plasmid

text: pBluescript SK+


text: Texas A&M University

text: live

text: Generic

text: thyroid and parathyroid

text: Bos taurus

text: NotI

text: EcoRI

text: thyroid and parathyroid

text: CSEQFN23 thyroid and parathyroid

text: Plasmid

text: pBluescript SK+


text: Texas A&M University

text: live

text: Generic

text: thyroid and parathyroid

text: Bos taurus

text: NotI

text: EcoRI

text: thyroid and parathyroid

text: CSEQFN22 thyroid and parathyroid

text: Plasmid

text: pBluescript SK+


text: Texas A&M University

text: live

text: Generic

text: stomach-abomasum

text: Bos taurus

text: NotI

text: EcoRI

text: stomach-abomausum

text: CSEQFXL07 stomach-abomasum

text: Plasmid

text: pBluescript SK+


text: Texas A&M University

text: live

text: Generic

text: thyroid and parathyroid

text: Bos taurus

text: NotI

text: EcoRI

text: thyroid and parathyroid

text: CSEQFXL15 thyroid and parathyroid

text: Plasmid

text: pBluescript SK+


text: Texas A&M University

text: live

text: Generic

text: stomach-reticulum

text: Bos taurus

text: NotI

text: EcoRI

text: stomach-reticulum

text: CSEQFXL08 stomach-reticulum

text: Plasmid

text: pBluescript SK+


text: Texas A&M University

text: live

text: Generic

text: thyroid and parathyroid

text: Bos taurus

text: NotI

text: EcoRI

text: thyroid and parathyroid

text: CSEQFN43 thyroid and parathyroid

text: Plasmid

text: pBluescript SK+


text: Texas A&M University

text: live

text: Generic

text: thyroid and parathyroid

text: Bos taurus

text: NotI

text: EcoRI

text: thyroid and parathyroid

text: CSEQFN46 thyroid and parathyroid

text: Plasmid

text: pBluescript SK+


text: Texas A&M University

text: live

text: Generic

text: hypothalamus

text: Bos taurus

text: NotI

text: EcoRI

text: hypothalamus

text: CSEQFXN08 hypothalamus

text: Plasmid

text: pBluescript SK+


text: Texas A&M University

text: live

text: Generic

text: pooled stomach tissues

text: Bos taurus

text: NotI

text: EcoRI

text: rumen, omasum, abomasum, reticulum

text: CSEQFXN40 pool of stomach libraries

text: Plasmid

text: pBluescript SK+


text: Texas A&M University

text: live

text: Generic

text: pooled stomach tissues

text: Bos taurus

text: NotI

text: EcoRI

text: rumen, omasum, abomasum, reticulum

text: CSEQFXN45 pool of stomach libraries

text: Plasmid

text: pBluescript SK+


text: Fred Hutchinson Cancer Research Center

text: live

text: Generic

text: This library was provided by Leslie Vosshall. mRNA was prepared from the olfactory and respiratory epithelium of an adult mouse. Oligo-dT primed cDNA was directionally cloned into Stratagene's lambdaZAPII-XR vector.

text: Adult

text: Olfactory turbinates

text: Mus musculus

text: EcoRI

text: XhoI

text: Olfactory and respiratory epithelium

text: Adult mouse olfactory epithelium library

text: Phage

text: LambdaZAPII-XR


text: Fred Hutchinson Cancer Research Center

text: live

text: Generic

text: This library was provided by Tyler Cutforth. mRNA was prepared from the olfactory and respiratory epithelium of mixed Swiss-Webster and C57BL/6 embryos, aged E16.5-E18.5. Oligo-dT primed cDNA was directionally cloned into Stratagene's lambdaZAPII-XR vector.

text: Olfactory turbinates

text: Mus musculus

text: EcoRI

text: XhoI

text: Mixed Swiss-Webster and C57BL/6

characteristic: strain

text: Olfactory and respiratory epithelium

text: Embryonic mouse olfactory epithelium library

text: Phage

text: LambdaZAPII-XR


text: National Cancer Institute / NIH

text: live

text: Generic

text: 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.6 kb (range 0.9-3.0 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA).

characteristic: description

text: metamorphosis stage 53

text: Developing Tadpole

text: Xenopus laevis

text: Sfi

text: Sfi

text: plasmid (chloramphenoicol resistant, grown at 30C)

text: pDNR-LIB


text: National Cancer Institute / NIH

text: live

text: Generic

text: 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.7 kb (range 0.8-3.0 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA).

text: metamorphosis stage 62

text: Developing Tadpole

text: Xenopus laevis

characteristic: organism

text: SfiI

text: SfiI

text: pDNR-LIB


text: Graduate School of Biotechnology, University of Korea

text: live

text: Generic

text: 4-weeks after germination

text: immature leaf

text: Oryza minuta

text: Oryza minuta HybriZAP-2.1 XR library


text: The First Hospital, Nanhua University

text: live

text: Generic

text: Carcinoma cell

characteristic: cell type

text: Using the technique of suppression subtractive hybridization (SSH) to construct a subtractive library containing differentially expressed genes fragments between Thyroid papillary carcinoma tissue and normal thyroid tissue, With PCR-Select™ cDNA Subtraction Kit (Clontech).

text: thyroid gland

text: Homo sapiens

text: EcoR I

text: male

characteristic: sex

text: Papillary Carcinoma

text: Human Thyroid Papillary Carcinoma Subtractive Library

text: plasmid

text: pT-Adv


characteristic: External Id

text: South China Agriculture University

text: live

text: Generic

text: The EST sequences were gotten from sequencing Lantang pig muscle cDNA Library. This sequence may serve as a candidate gene of meat quality.

text: adult

characteristic: dev-stage

text: Longissimus Dorsi Muscle

text: Sus scrofa

text: male

text: Lantang Pig

text: Swine Lantang Longissimus Dorsi Muscle cDNA Library


text: University of Minnesota

text: live

text: Generic

text: Jejunal Peyer's patches were isolated from healthy, 4-6 week old cross-bred pigs. RNA was extracted either immediately or after 3 hours stimulation in an Ussing chamber with one of the following treatments: 1) no treatment, 2) Salmonella choleraesuis vaccine strain SC-54, 3) phorbol myristate acetate, concanavalin A, and 8-bromo-cyclic AMP, and 4) lipopolysaccharide and cholera toxin. Each treatment was performed in the presence and absence of cycloheximide. Purified poly A + RNA from each of the treated tissues (2-4) was combined together, reverse transcribed, and cloned in to pCMVSPORT6 to make a library of approximately 530,000 recombinant clones with an average insert size of 1.0 kbp. Poly A+ RNA from freshly isolated and non-treated tissue from an Ussing chamber (treatment 1) was cloned in the same manner to produce an unstimulated cDNA library of approximately 900,000 clones with an average insert size of 1.5 kbp. Equal portions of the two libraries were pooled and then subtracted with porcine ST fibroblast RNA to create a subtracted porcine Peyer's patch library of approximately 6,000 clones with an average insert size of 1.0 kbp.

text: small intestine, jejunum

characteristic: organ

text: Sus scrofa

text: NotI

text: SalI

text: Peyer's patch

characteristic: title

text: plasmid


text: Michigan State University

characteristic: INSDC center name

text: live

text: Generic

text: Latex was obtained from mature rubber plants (H. brasiliensis clone RRIM 600) growing at the Rubber Research Institute of Malaysia (RRIM), Selangor, Malaysia. We used the SMART strategy (Switching Mechanism At 5' end of the RNA Transcript; Clontech, palo alto, CA) to construct a high-content full-length cDNA library from the latex according to the manufacturers' instructions. This method is based on intrinsic terminal transferase activity of a reverse transcriptase (RT). The phage library was converted through mass excision to a phagemid library in the vector pTriplEX

text: adult

text: E.coli (BM52.8)

characteristic: lab-host

text: trunk (stem)

text: Hevea brasiliensis

text: SfiIA

text: SfiIB

text: Mull. Arg. (the Brazilian rubber tree), clone RRIM 600

text: laticifer

text: Latex Lambda TriplEX cDNA Library


characteristic: INSDC first public

text: live

text: Generic

text: cDNA made by oligo-dT priming and directionally cloned. 5' and 3' adaptors were used in cloning as follows: 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' and 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)NN-3'. Full-length enriched library was constructed using the Clontech Creator SMART kit and size-selected to contain the 0.5 kb size fraction. Library created in the laboratory of M. Brownstein (NIMH, NIH). Note: this is a NIH_MGC Library.

text: DH10B (T1-phage-resistant)

text: lung and heart

text: Mus musculus

text: SfiI (ggccattatggcc)

text: SfiI (ggccgcctcggcc)

text: plasmid (chloramphenicol resistant, grown at 30C)

text: pDNR-LIB

characteristic: vector


text: Purdue University

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: whole organism (engorged adult)

text: Pediculus humanus corporis

text: Body louse cDNA library


text: live

text: Generic

characteristic: NCBI submission model

text: Library is oligo-dT primed and directionally cloned. cDNA was prepared from a glandular pool of tissues from thyoid, parathyroid, adrenal, cortex and pineal gland. 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.38 kb (range 0.60-3.5 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA). Note: this is a NIH_MGC Library.

text: DH10B (T1 phage-resistant)

text: Pooled-Glandular

text: Homo sapiens

text: SfiI (ggccattatggcc)

text: SfiI (ggccgcctcggcc)

text: pDNR-LIB


text: National Cancer Institute / NIH

text: live

text: Generic

characteristic: NCBI submission package

text: 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.4 kb (range 0.5-4.0 kb). 14/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA). Note: this is a NIH_MGC Library.

text: DH10B (T1 phage-resistant)

text: Blood vessels - aorta, basilar and artery

text: Homo sapiens

text: SfiI (ggccattatggcc)

text: SfiI (ggccgcctcggcc)

text: pDNR-LIB


text: USDA-Agricultural Research Service

text: live

text: Generic

text: peripheral blood leucocyte

characteristic: cell-line

text: Primary library

text: adult

text: peripheral blood

text: Ictalurus punctatus

text: Sal 1

text: Not 1

text: commercial

characteristic: strain

text: plasmid


text: UC Davis, Plant Pathology

text: live

text: Generic

text: Cabernet Sauvignon

characteristic: cultivar

text: CAB1 is a cDNA library of Vitis vinifera cv. 'Cabernet Sauvignon' Clone 8 flowers. Samples were collected approximately eleven days before onset of bloom (clusters at this stage were fully developed and flowers with calyptras or caps still attached. Sampled vines were located at the University of California, Davis, Experimental Vineyard. cDNAs were made by oligo-dT priming and directionally cloned. 5'and 3' adaptors were used in cloning as follows: 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' and 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)NN-3'. Library was constructed using the Clontech Creator SMART kit and size-selected to contain the 0.5-3 kb size fraction.

characteristic: description

text: Pre-bloom

text: Flower - Pre-bloom

text: Vitis vinifera

characteristic: organism

text: SfiI

text: SfiI

text: Hermaphrodite

text: Cabernet Sauvignon Flower Pre-bloom - CAB1

text: Plasmid

text: pDNR


characteristic: External Id

text: National Cancer Institute / NIH

text: live

text: Generic

text: Library is oligo-dT primed and directionally cloned. cDNA was prepared from a pooled samples of tissues from Skin, meninges, duramatter, pia matter and choroid plexus. 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.47 kb (range 0.50-4.0 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA). Note: this is a NIH_MGC Library

text: DH10B (T1 phage-resistant)

text: Pooled-Skin

text: Homo sapiens

text: SfiI (ggccattatggcc)

text: SfiI (ggccgcctcggcc)

text: pDNR-LIB


text: The University of Georgia, Department of Plant Biology

text: live

text: Generic

text: leukocytes

characteristic: cell type

text: The library was prepared from polyA+ RNA from unstimulated equine peripheral blood leukocytes isolated from a healthy adult horse. Double-stranded cDNA was cloned unidirectionally into different DraIII sites of the pME18S-FL3 vector (5-prime DraIII site is CACTGTGTG, 3-prime DraIII site is CACCATGTG). XhoI excises the cDNA insert.

text: DH10B-T1 phage-resistant E. coli

text: circulatory system

text: Equus caballus

text: XhoI

text: XhoI

text: male

characteristic: sex

text: Polish Arabian

text: blood

characteristic: tissue-type

text: Unstimulated peripheral blood leukocytes N4

text: Plasmid


characteristic: INSDC center name

text: live

text: Generic

text: leukocytes

text: DH10B-T1 phage-resistant E. coli

text: circulatory system

characteristic: organ

text: Equus caballus

text: XhoI

text: XhoI

text: female

text: Belgian draft

text: blood

text: Unstimulated peripheral blood leukocytes N1

characteristic: title

text: Plasmid


characteristic: INSDC first public

text: live

text: Generic

text: leukocytes

text: DH10B-T1 phage-resistant E. coli

characteristic: lab-host

text: circulatory system

text: Equus caballus

text: XhoI

text: XhoI

text: male

text: thoroughbred

text: blood

text: Unstimulated peripheral blood leukocytes N2

text: Plasmid

characteristic: vector


characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: leukocytes

text: The library was prepared from polyA+ RNA from equine peripheral blood leukocytes isolated from a healthy adult horse. The leukocytes were stimulated for 4 hr with 10 ng/ml E. coli O55:B5 LPS. Double-stranded cDNA was cloned unidirectionally into different DraIII sites of the pME18S-FL3 vector (5-prime DraIII site is CACTGTGTG, 3-prime DraIII site is CACCATGTG). XhoI excises the cDNA insert.

text: DH10B-T1 phage-resistant E. coli

text: circulatory system

text: Equus caballus

text: XhoI

text: XhoI

text: female

text: Belgian draft

text: blood

text: Stimulated peripheral blood leukocytes S1

text: Plasmid


text: live

text: Generic

characteristic: NCBI submission package

text: leukocytes

text: DH10B-T1 phage-resistant E. coli

text: circulatory system

text: Equus caballus

text: XhoI

text: XhoI

text: female

text: Tennesee walking horse

characteristic: strain

text: blood

text: Stimulated peripheral blood leukocytes S3

text: Plasmid


text: live

text: Generic

text: leukocytes

characteristic: description

text: DH10B-T1 phage-resistant E. coli

text: circulatory system

text: Equus caballus

text: XhoI

text: XhoI

text: male

text: thoroughbred

text: blood

text: Stimulated peripheral blood leukocytes S2

text: Plasmid


text: live

text: Generic

text: leukocytes

text: DH10B-T1 phage-resistant E. coli

text: circulatory system

text: Equus caballus

characteristic: organism

text: XhoI

text: XhoI

text: male

text: Polish Arabian

text: blood

text: Stimulated peripheral blood leukocytes S4

text: Plasmid


characteristic: External Id

text: live

text: Generic

text: leukocytes

text: DH10B-T1 phage-resistant E. coli

text: circulatory system

text: Equus caballus

text: XhoI

text: XhoI

text: male

text: Dartmoor Pony

text: blood

text: Stimulated peripheral blood leukocytes S5

text: Plasmid


text: live

text: Generic

text: leukocytes

characteristic: cell type

text: DH10B-T1 phage-resistant E. coli

text: circulatory system

text: Equus caballus

text: XhoI

text: XhoI

text: male

text: quarterhorse

text: blood

text: Stimulated peripheral blood leukocytes S6

text: Plasmid


text: Instituto de Ciencia Aplicada e Tecnologia

text: live

text: Generic

text: Nugget

characteristic: cultivar

text: cDNA library constructed using the ZAP express cDNA synthesis kit and the Gigapack III Gold Cloning kit.

text: In vitro plants and organogenic nodules

characteristic: dev-stage

text: Internodes and nodules

text: Humulus lupulus

text: female

characteristic: sex

text: Hop Lambda ZAP Express cDNA Library


characteristic: INSDC center name

text: live

text: Generic

text: leukocytes

text: DH10B-T1 phage-resistant E. coli

text: circulatory system

characteristic: organ

text: Equus caballus

text: XhoI

text: XhoI

text: female

text: Tennesee walking horse

text: blood

characteristic: tissue-type

text: Unstimulated peripheral blood leukocytes N3

text: Plasmid


characteristic: INSDC first public

text: live

text: Generic

text: leukocytes

text: DH10B-T1 phage-resistant E. coli

characteristic: lab-host

text: circulatory system

text: Equus caballus

text: XhoI

text: XhoI

text: male

text: Dartmoor Pony

text: blood

text: Unstimulated peripheral blood leukocytes N5

characteristic: title

text: Plasmid


characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: leukocytes

text: DH10B-T1 phage-resistant E. coli

text: circulatory system

text: Equus caballus

text: XhoI

text: XhoI

text: male

text: quarterhorse

text: blood

text: Unstimulated peripheral blood leukocytes N6

text: Plasmid

characteristic: vector


text: Cornell University

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Landsberg erecta (Ler)

text: Senescent rosette leaves #5 and #6 (counted from the bottom) were harvested and immediately frozen in liquid N2. The leaves were visibly yellow excepted for the leaf base areas that were still greenish.

text: Yellow Leaf With Greenish Base Area

text: E. coli

text: Rosette Leaf

text: Arabidopsis thaliana

text: EcoRI

text: EcoRI

text: Leaf

text: Arabidopsis Leaf Senescence Library

text: Plasmid

text: pBluscript SKII+


text: National Cancer Institute / NIH

text: live

text: Generic

text: Non-normalized full-length enriched library 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.08 kb (range .73-1.37 kb). 13/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA).Corp.

characteristic: description

text: DH10B (phage-resistant)

text: brain - normal mediobasal hypothalamus

text: Mus musculus

characteristic: organism

text: SfiI (ggccattatggcc)

text: SfiI (ggccgcctcggcc)

text: plasmid (chloramphenicol resistant, grown at 30C)

text: pDNR-LIB


characteristic: External Id

text: National Cancer Institute / NIH

text: live

text: Generic

text: Non-normalized full-length enriched library 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.71 kb (range 0.5-3.0 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA) Corp.

text: DH10B (phage-resistant)

text: Pooled - Molar

text: Mus musculus

text: SfiI (ggccattatggcc)

text: SfiI (ggccgcctcggcc)

text: pDNR-LIB


text: The Institute for Genomic Research

text: live

text: Generic

text: Salivary glands were dissected on day four after initiation of feeding. Total RNA was prepared using acid guanidium thiocyanate-phenol-chloroform extraction. The cDNA library was custom prepared by Invitrogen Corporation. Briefly, first strand cDNA was primed using oligo(dT) containing a NotI site. Size fractionated double stranded cDNA was ligated to EcoRV-NotI cleaved vector and electroporated into E.coli. Library RAA was made from uninfected ticks.

text: Adult

text: E. coli strain DH10B-TonA

text: Salivary glands

text: Rhipicephalus appendiculatus

text: Muguga

characteristic: strain

text: RAA


text: The Institute for Genomic Research

text: live

text: Generic

text: Salivary glands were dissected on day four after initiation of feeding. Total RNA was prepared using acid guanidium thiocyanate-phenol-chloroform extraction. The cDNA library was custom prepared by Invitrogen Corporation. Briefly, first strand cDNA was primed using oligo(dT) containing a NotI site. Size fractionated double stranded cDNA was ligated to EcoRV-NotI cleaved vector and electroporated into E.coli. Library RAB was made from ticks infected with Theileria parva.

text: Adult

characteristic: dev-stage

text: E. coli strain DH10B-TonA

text: Salivary glands

text: Rhipicephalus appendiculatus

text: Muguga

text: RAB


text: UMR CNRS 6161, Universite de Poitiers

text: live

text: Generic

text: Ugni blanc

characteristic: cultivar

text: Oriented library

text: Veraison stage

text: Fruit without seeds

text: Vitis vinifera

text: SalI

text: NotI

text: Veraison Grape Berries Psport1 Library

text: plasmid


text: UMR CNRS 6161, Universite de Poitiers

characteristic: INSDC center name

text: live

text: Generic

text: Chardonnay

text: Oriented library

text: Veraison stage

text: Fruit without seeds

text: Vitis vinifera

text: SfiIA

text: SfiIB

text: Veraison Grape Berries Lambda Triplex2 Library

text: Phage

text: Lambda Triplex2


text: Universite de Bordeaux I, Institut National de la Recherche Agronomique

text: live

text: Generic

text: Chardonnay

text: Oriented library

text: Green stage

text: Fruit without seeds

characteristic: organ

text: Vitis vinifera

text: SfiIA

text: SfiIB

text: Green Grape Berries Lambda Triplex2 Library

text: Phage

text: Lambda Triplex2


text: UMR CNRS 6161, Universite de Poitiers

text: live

text: Generic

text: Chardonnay

text: Oriented library

text: Ripe stage

text: Fruit pedicle

text: Vitis vinifera

text: SfiIA

text: SfiIB

text: Ripe Grape (harvested at 5 p.m.) Pedicles Lambda Triplex2 Library

text: Phage

text: Lambda Triplex2


text: UMR CNRS 6161, Universite de Poitiers

text: live

text: Generic

text: Chardonnay

text: Oriented library

text: Ripe stage

text: Fruit pedicle

text: Vitis vinifera

text: SfiIA

text: SfiIB

text: Ripe Grape (harvested at 9 a.m.) Pedicles Lambda Triplex2 Library

text: Phage

text: Lambda Triplex2


text: UMR CNRS 6161, Universite de Poitiers

text: live

text: Generic

text: Chardonnay

text: Oriented library

text: Green stage

text: Fruit pedicle

text: Vitis vinifera

text: SfiIA

text: SfiIB

text: Green Grape (harvested at 9 a.m.) Pedicles Lambda Triplex2 Library

text: Phage

text: Lambda Triplex2


text: UMR CNRS 6161, Universite de Poitiers

text: live

text: Generic

text: Chardonnay

text: Oriented library

text: Ripe stage

text: Fruit without seeds

text: Vitis vinifera

text: SfiIA

text: SfiIB

text: Ripe Grape Berries Lambda Triplex2 Library

text: Phage

text: Lambda Triplex2


text: UMR CNRS 6161, Universite de Poitiers

text: live

text: Generic

text: Chardonnay

text: Oriented library

text: Green stage

text: Fruit pedicle

text: Vitis vinifera

text: SfiIA

text: SfiIB

text: Green Grape (harvested at 5 p.m.) Pedicles Lambda Triplex2 Library

text: Phage

text: Lambda Triplex2