text: ENCODE DCC

characteristic: INSDC last update

text: live

text: Generic

characteristic: NCBI submission model

text: Lonza

text: HMVEC-dBl-Ad

text: Human

text: F

text: Homo sapiens

text: HMVEC-dBl-Ad


status: PUBLIC

characteristic: External Id

text: ENCODE DCC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC secondary accession

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission package

characteristic: SRA accession

text: WiCell

tag: attribute

characteristic: biomaterial provider

characteristic: cell

text: Undifferentiated human embryonic stem cells

characteristic: cell description

text: Human

characteristic: cell organism

text: U

characteristic: cell sex

text: Homo sapiens

characteristic: organism

characteristic: replicate

characteristic: title


text: ENCODE DCC

characteristic: INSDC last update

text: live

text: Generic

text: Lonza

text: HMVEC-dNeo

text: Normal Neonatal Human Microvascular Endothelial Cells (single Donnor), Dermal-Derived

text: Human

text: M

text: Homo sapiens

text: HMVEC-dNeo


text: ENCODE DCC

text: live

text: Generic

characteristic: NCBI submission model

text: Lonza

text: NHLF

text: Normal Human Lung Fibroblasts

text: Human

text: U

text: Homo sapiens

text: NHLF


characteristic: External Id

text: ENCODE DCC

text: live

text: Generic

text: Dr. Carl Mann, SBIGeM

text: Embryonic Lung Fibroblast Cells, hTERT immortalized, includes Raf1 construct

text: Normal (RAF)

characteristic: cell karyotype

text: Fibroblast

characteristic: cell lineage

text: human

text: F

text: Homo sapiens


text: ENCODE DCC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC secondary accession

text: live

text: Generic

text: ScienCell

text: HPAF

text: Human Pulmonary Artery Fibroblasts

text: Human

text: U

text: Homo sapiens

text: HPAF


text: ENCODE DCC

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission package

characteristic: SRA accession

text: ScienCell

text: HIPEpiC

text: Human Iris Pigment Epithelial Cells

text: ectoderm

text: Human

text: U

text: Homo sapiens

text: HIPEpiC


text: ENCODE DCC

text: live

text: Generic

text: Coriell http://ccr.coriell.org/Sections/Search/Search.aspx?PgId=165&q=AG09309

characteristic: biomaterial provider

characteristic: cell

text: Adult human toe fibroblast

characteristic: cell description

text: mesoderm

text: Human

text: F

text: Homo sapiens


text: ENCODE DCC

text: live

text: Generic

text: Lonza

text: SAEC

text: Small Airway Epithelial Cells

text: normal

text: Human

characteristic: cell organism

text: U

characteristic: cell sex

text: Homo sapiens

characteristic: organism

text: SAEC


text: ENCODE DCC

characteristic: INSDC last update

text: live

text: Generic

text: ScienCell

text: HRGEC

text: Human Renal Glomerular Endothelial Cells

text: Normal

text: Endothelial

text: human

text: U

text: Homo sapiens

characteristic: replicate

text: HRGEC

characteristic: title


characteristic: External Id

text: ENCODE DCC

text: live

text: Generic

characteristic: NCBI submission model

text: Coriell http://ccr.coriell.org/Sections/Search/Search.aspx?PgId=165&q=AG09319

text: Adult human gum tissue fibroblasts

text: mesoderm

text: Human

text: F

text: Homo sapiens


text: ENCODE DCC

characteristic: INSDC center name

text: live

text: Generic

text: Coriell; http://ccr.coriell.org/Sections/Search/Search.aspx?PgId=165&q=GM12878

text: immortalized cell line

characteristic: biomaterial type

text: lymphoblastoid

text: relatively normal

characteristic: cell karyotype

text: International HapMap Project - CEPH/Utah - European Caucasion; Epstein-Barr Virus

characteristic: cell lineage

text: Human

text: F

characteristic: line

text: Homo sapiens


text: ENCODE DCC

characteristic: INSDC first public

characteristic: INSDC secondary accession

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission package

characteristic: SRA accession

text: ScienCell

text: HNPCEpiC

text: Human Non-Pigment Ciliary Epithelial Cells

text: Human

text: U

text: Homo sapiens

text: HNPCEpiC


text: ENCODE DCC

text: live

text: Generic

text: ATCC

characteristic: biomaterial provider

characteristic: cell

text: Human neuroblastoma

characteristic: cell description

text: ectoderm

text: Human

characteristic: cell organism

text: M

characteristic: cell sex

text: Homo sapiens


characteristic: External Id

text: ENCODE DCC

characteristic: INSDC last update

text: live

text: Generic

text: ScienCell

text: HRPEpiC

text: Human Retinal Pigment Epithelial Cells

text: ectoderm

text: Human

text: U

text: Homo sapiens

characteristic: organism

characteristic: replicate

text: HRPEpiC

characteristic: title


text: ENCODE DCC

characteristic: INSDC center name

text: live

text: Generic

characteristic: NCBI submission model

text: ScienCell

text: HA-sp

text: Human astrocytes spinal cord

text: Normal

characteristic: cell karyotype

text: Astrocytic

characteristic: cell lineage

text: human

text: U

text: Homo sapiens

text: HA-sp


text: ENCODE DCC

characteristic: INSDC first public

characteristic: INSDC secondary accession

text: live

characteristic: INSDC status

text: Generic

text: ATCC

text: Human promyelocytic leukemia cells. (PMID: 276884)

text: cancer

text: Human

text: F

text: Homo sapiens


text: ENCODE DCC

text: live

text: Generic

characteristic: NCBI submission package

characteristic: SRA accession

text: Lonza

characteristic: biomaterial provider

text: HMVEC-LLy

text: Normal Human Lymphatic Microvascular Endothelial Cells, Lung-Derived

text: Human

text: F

text: Homo sapiens

text: HMVEC-LLy


text: ENCODE DCC

text: live

text: Generic

text: ScienCell

text: HMF

characteristic: cell

text: human mammary fibroblasts

characteristic: cell description

text: Human

characteristic: cell organism

text: F

text: Homo sapiens

text: HMF


characteristic: External Id

text: ENCODE DCC

characteristic: INSDC last update

text: live

text: Generic

text: ATCC

text: leukemia

text: cancer

text: The continuous cell line K-562 was established by Lozzio and Lozzio from the pleural effusion of a 53-year-old female with chronic myelogenous leukemia in terminal blast crises. - ATCC

text: Human

text: F

characteristic: cell sex

text: Homo sapiens


text: ENCODE DCC

text: live

text: Generic

text: ScienCell

text: HAEpiC

text: Human Amniotic Epithelial Cells

text: Human

text: U

text: Homo sapiens

characteristic: organism

characteristic: replicate

text: HAEpiC

characteristic: title


text: ENCODE DCC

characteristic: INSDC center name

text: live

text: Generic

characteristic: NCBI submission model

text: Lonza

text: HPdLF

text: Normal Human Periodontal Ligament Fibroblasts

text: Human

text: M

text: Homo sapiens

text: HPdLF


text: ENCODE DCC

characteristic: INSDC first public

text: live

text: Generic

text: Coriell http://ccr.coriell.org/Sections/Search/Search.aspx?PgId=165&q=AG10803

text: Adult human abdominal skin fibroblasts

text: mesoderm

characteristic: cell lineage

text: Human

text: M

text: Homo sapiens


text: ENCODE DCC

characteristic: INSDC secondary accession

text: live

characteristic: INSDC status

text: Generic

text: Lonza

text: HMEC

text: Human Mammary Epithelial Cells

text: Human

text: U

text: Homo sapiens

text: HMEC


text: ENCODE DCC

text: live

text: Generic

characteristic: NCBI submission package

characteristic: SRA accession

text: Coriell; http://ccr.coriell.org/Sections/Search/Search.aspx?PgId=165&q=GM06990

text: Lymphoblastoid, International HapMap Project, CEPH/Utah, Treatment: Epstein-Barr Virus transformed

text: normal

characteristic: cell karyotype

text: mesoderm

text: Human

text: F

text: Homo sapiens


text: ENCODE DCC

text: live

text: Generic

text: ATCC

characteristic: biomaterial provider

characteristic: cell

text: retinoblastoma (PMID: 844036)

characteristic: cell description

text: Human

text: F

text: Homo sapiens


characteristic: External Id

text: ENCODE DCC

characteristic: INSDC last update

text: live

text: Generic

text: ATCC

text: liver carcinoma

text: cancer

text: endoderm

text: Human

characteristic: cell organism

text: M

text: Homo sapiens


text: ENCODE DCC

text: live

text: Generic

text: WiCell

text: Human

text: U

characteristic: cell sex

text: Homo sapiens

characteristic: organism

characteristic: replicate


Homolaphlyctis polyrhiza

text: University of Idaho, Department of Biological Sciences

characteristic: INSDC center name

text: live

text: Generic

characteristic: NCBI submission model

text: Homolaphlyctis polyrhiza

characteristic: description

text: Homolaphlyctis polyrhiza JEL 142

text: Homolaphlyctis polyrhiza

characteristic: title


text: Institute for Genome Sciences University of MD School of Medicine

characteristic: INSDC first public

characteristic: INSDC secondary accession

text: live

characteristic: INSDC status

text: Generic

text: Staphylococcus aureus subsp. aureus CIG1057

characteristic: strain

text: 1057 StaphylococcusaureusCIG1057


ITS2 project

text: implad

text: live

text: Generic

characteristic: NCBI submission package

characteristic: SRA accession

text: The PCR mixture was sequenced in separate half-lanes of a picotiter plate using a GS_FLX pyrosequencer according to the manufacturer's instructions, obtained from 247 samples from 178 species of 76 genera belonging to 44 families of angiosperms, gymnosperms, and ferns. Therefore, The NCBI Taxon ID 4054 of Panax ginseng was used to represent the all samples.

text: Panax ginseng

text: ITS2 variants as molecular fossils in plant genomes


text: Pacific Northwest National Labs

text: live

text: Generic

text: default sample for a study of surface waters off the Delaware coast.

text: marine metagenome


Bacteria in Drinking Water

text: University of Tennessee

text: live

text: Generic

text: 7 water samples and 9 biofilm samples were surveyed by construction 16S rRNA amplicon libraries.The amplicons were pooled together with barcode labeled on both end as shown in the following. Forward AACCATGC Forward ACACAGAG Forward AACGCGTT Forward ACTCAGTG Forward AGACCACT Forward CGTTCGTT Forward CTGTCTGT Forward CGTAATGC Forward CAGTCTCT Forward CCAATACG Reverse GACAGACA Reverse GAAGCAAC

text: ecological metagenomes

text: Bacterial Community Composition in Drinking Water Revealed by Pyrosequencing Analysis of 16S rRNA Genes


text: live

text: Generic

text: Staphylococcus aureus subsp. aureus CIG1096

text: 1096 StaphylococcusaureusCIG1096


text: UMIGS

text: live

text: Generic

text: Vibrio cholerae HC-81A1

text: HC-81A1 HC-81A1


text: Buell Lab, Dept. of Plant Biology, Michigan State University

text: live

text: Generic

text: Globisporangium ultimum var. sporangiiferum

text: Pythium ultimum var sporangiiferum BR650 (=CBS 219.65)


text: live

text: Generic

text: Pythium aphanidermatum

text: Pythium aphanidermatum BR444


text: live

text: Generic

text: Pythium arrhenomanes

text: Pythium arrhenomanes Lev1578


text: live

text: Generic

text: Globisporangium irregulare

text: Pythium irregulare CBS 250.28=BR486


text: live

text: Generic

text: Globisporangium iwayamae

text: Pythium iwayamai isolate P174


text: live

text: Generic

text: Phytopythium vexans

text: Pythium vexans CBS 119.80=BR484


text: Chinese Research Academy For Environment Sciences

text: live

text: Generic

text: 1.5 microliter RNA solution (1-3 microgram of total RNA) was normalized using CDS and SMART Oligo IV primer firstly, and then the reaction solution was reversed transcription for synthesizing the first-strand using MMLV reverse transcriptase (Promega, USA). Subsequently, PCR reaction was operated and cDNA was extracted using Promegas PCR clean-up system kit (Madison, USA). And then the cDNA library was constructed using SMART cDNA Library Construction Kit. After that, 6000 random clones were sequenced by the ABI3730xl. BLAST homology searches in the nr and nt databases in GenBank were performed for each of the unigenes under Evalues of 1e-5. The unigenes were named after the homologous sequences in GenBank. The annotated unigenes were then matched to specific processes or functions using COG and GO tools.

text: mature

characteristic: dev-stage

text: Sinotaia aeruginosa

text: gonads; digestive gland; peripheral tissue

characteristic: tissue-type

text: Bellamya aeruginosa normalized cDNA library

text: Plasmid

text: Lambda TripEx2

characteristic: vector


characteristic: External Id

text: Penn State University

characteristic: INSDC last update

text: live

text: Generic

text: This sample represents a pooled sample consisting of coral larvae collected from multiple locations and treatments. Acropora palmata larvae were acquired from colonies at multiple locations in the upper Florida Keys, Puerto Rico, and Curacao, and exposed to control and stressful conditions including three temperatures and elevated CO2 levels.

text: Acropora palmata

text: Gene discovery in the threatened Elkhorn coral: 454 sequencing of the Acropora palmata transcriptome


text: UMIGS

text: live

text: Generic

text: Vibrio cholerae HC-7A1

characteristic: organism

text: HC-7A1 HC-7A1


text: Institute for Genome Sciences University of MD School of Medicine

text: live

text: Generic

text: Vibrio cholerae HC-43A1

text: HC-43A1 HC-43A1


text: live

text: Generic

text: Vibrio cholerae HC-20A2

text: HC-20A2 HC-20A2


text: live

text: Generic

text: Pythium arrhenomanes

text: Pythium arrhenomanes; Pooled sample from five growth conditions (Yeast extract, Plich medium, heat stress, cold stress and mefenoxam)


text: live

text: Generic

text: Globisporangium irregulare

text: Pythium irregulare; Pooled sample from five growth conditions (Yeast extract, Plich medium, heat stress, cold stress and mefenoxam)


text: live

text: Generic

text: Globisporangium iwayamae

text: Pythium iwayamai; Pooled sample from five growth conditions (Yeast extract, Plich medium, heat stress, cold stress and mefenoxam)


text: live

text: Generic

text: Phytopythium vexans

text: Pythium vexans; Pooled sample from five growth conditions (Yeast extract, Plich medium, heat stress, cold stress and mefenoxam)


text: University of Missouri

text: live

text: Generic

text: Cultivar/line: Maize inbred B73 Dates grown: late July - early August, 2008 Environment grown in: BPP Field Lab, Corvallis, OR Developmental stage: 9-day old Date of tissue isolation: August 6, 11:30 am; Temp: 22.4°C; Relative humidity 56% Method of tissue isolation: Seedling cut off at first node (includes mature sheath &amp; blade, immature leaves, possibly stem &amp; meristem), placed in liquid nitrogen. Number of individuals pooled in sample: 1 Date of RNA Isolation: 8/25/08 Method of RNA isolation: Tissue ground in liquid nitrogen, processed using Trizol (invitrogen) RNA preparation method. RNEasy MinElute columns (Qiagen) were used to purify total RNA from the aqueous phase from the Trizol prep. Resultant RNA was assessed by Bioanalyzer. Bioanalyzer RNA Concentration &amp; RNA Integrity Number (RIN): SeedlingA or Seedling1 - 1761 ng/ul; 9.9 Date of cDNA preparation: 3/30/10 Method of cDNA preparation: SMART PCR cDNA Synthesis Kit with SMART MMLV Reverse Transcriptase and the Advantage 2 PCR kit (Clontech). cDNA synthesis adapter primers: The 3’ polyA tail for each transcript is used to anneal to the primer for cDNA synthesis from the SMART CDS Primer II A : 5’-AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTVN-3’ ( V=A, C, or G). The 5’ end of each transcript is associated with addition of 3-5 dC residues to the cDNA strand, due to the terminal transferase activity of MMLV-RT. The three dG’s at the 3’ end of the SMART II A oligonucleotide adapter (5’-AAGCAGTGGTATCAACGCAGAGTACGCGGG-3’) anneal to these additional dC’s, and this adapter is used to create the double stranded cDNA via PCR. As per the SMART PCR protocol, an Advantage 2 PCR kit (Clontech) was used to amplify the cDNA for 15 PCR cycles as the final step.

text: Zea mays


text: Washington University, Genome Sequencing Center

text: live

text: Generic

text: Caenorhabditis afra

text: Generic sample from Caenorhabditis sp. 7 MAF-2007


text: live

text: Generic

text: Caenorhabditis tropicalis

text: Generic sample from Caenorhabditis sp. 11 MAF-2010


text: live

text: Generic

text: Caenorhabditis japonica

text: Sample from Caenorhabditis japonica


text: live

text: Generic

text: Caenorhabditis nigoni

text: Generic sample from Caenorhabditis sp. 9 MAF-2010


text: CGS-GL

text: live

text: Generic

text: n

text: Sample 5 from Uncultured Fecal (human) sample

text: FMA:Feces

characteristic: body product

text: FMA:Feces

characteristic: body site

text: USA

characteristic: country

text: N/D

characteristic: diet

characteristic: elevation

text: ENVO:feces

characteristic: env biome

text: ENVO:feces

characteristic: env feature

text: ENVO:feces

characteristic: env matter

characteristic: host subject id

characteristic: latitude

characteristic: longitude

text: human gut metagenome

text: anaerobe

text: Culturing Microbiota

characteristic: project name

text: n

characteristic: public

text: m

characteristic: sex

text: N/D


characteristic: External Id

text: CGS-GL

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC secondary accession

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

characteristic: SRA accession

text: n

text: Sample 6 from Cultured Plate (human) sample.

text: FMA:Feces

text: FMA:Feces

text: USA

characteristic: description

text: N/D

text: ENVO:feces

text: ENVO:feces

text: ENVO:feces

text: human gut metagenome

text: anaerobe

text: Culturing Microbiota

text: n

text: m

text: N/D

characteristic: title


text: CGS-GL

characteristic: INSDC last update

text: live

text: Generic

text: n

text: Sample 66-70 from Uncultured Fecal (mouse) sample on LF/PP diet

text: FMA:Feces

characteristic: body product

text: FMA:Feces

characteristic: body site

text: USA

characteristic: country

text: LF/PP

characteristic: diet

characteristic: elevation

text: ENVO:feces

characteristic: env biome

text: ENVO:feces

characteristic: env feature

text: ENVO:feces

characteristic: env matter

text: mouse gut metagenome

characteristic: organism

text: anaerobe

text: Culturing Microbiota

text: n

text: m

text: LF/PP


characteristic: External Id

text: CGS-GL

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC secondary accession

text: live

text: Generic

text: n

text: Sample 265-268 from Uncultured Fecal (mouse) sample on Western diet

text: FMA:Feces

text: FMA:Feces

text: USA

text: Western

text: ENVO:feces

text: ENVO:feces

text: ENVO:feces

characteristic: host subject id

characteristic: latitude

characteristic: longitude

text: mouse gut metagenome

text: anaerobe

text: Culturing Microbiota

characteristic: project name

text: n

characteristic: public

text: m

characteristic: sex

text: Western


text: CGS-GL

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

characteristic: SRA accession

text: n

text: Sample 74-78 from Cultured Fecal (mouse) sample on LF/PP diet

text: FMA:Feces

characteristic: body product

text: FMA:Feces

characteristic: body site

text: USA

characteristic: country

characteristic: description

text: LF/PP

text: ENVO:feces

text: ENVO:feces

text: ENVO:feces

text: mouse gut metagenome

text: anaerobe

text: Culturing Microbiota

text: n

text: m

text: LF/PP

characteristic: title


characteristic: External Id

text: CGS-GL

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC secondary accession

text: live

text: Generic

text: n

text: Sample 272-276 from Cultured Fecal (mouse) sample on Western diet

text: FMA:Feces

text: FMA:Feces

text: USA

text: Western

characteristic: diet

characteristic: elevation

text: ENVO:feces

characteristic: env biome

text: ENVO:feces

characteristic: env feature

text: ENVO:feces

characteristic: env matter

text: mouse gut metagenome

characteristic: organism

text: anaerobe

text: Culturing Microbiota

text: n

text: m

text: Western


text: CGS-GL

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: n

text: Sample 2 from Uncultured Fecal (human) sample.

text: FMA:Feces

text: FMA:Feces

text: USA

text: N/D

text: ENVO:feces

text: ENVO:feces

text: ENVO:feces

characteristic: host subject id

characteristic: latitude

characteristic: longitude

text: human gut metagenome

text: anaerobe

text: Culturing Microbiota

characteristic: project name

text: n

characteristic: public

text: m

characteristic: sex

text: N/D


characteristic: External Id

text: CGS-GL

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC secondary accession

text: live

text: Generic

characteristic: SRA accession

text: n

text: Sample 3 from Cultured Plate (human) sample.

text: FMA:Feces

characteristic: body product

text: FMA:Feces

characteristic: body site

text: USA

characteristic: country

characteristic: description

text: N/D

text: ENVO:feces

text: ENVO:feces

text: ENVO:feces

text: human gut metagenome

text: anaerobe

text: Culturing Microbiota

text: n

text: m

text: N/D

characteristic: title


text: CGS-GL

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: n

text: Sample 61-65 from Uncultured Fecal (mouse) sample on LF/PP diet

text: FMA:Feces

text: FMA:Feces

text: USA

text: LF/PP

characteristic: diet

characteristic: elevation

text: ENVO:feces

characteristic: env biome

text: ENVO:feces

characteristic: env feature

text: ENVO:feces

characteristic: env matter

text: mouse gut metagenome

characteristic: organism

text: anaerobe

text: Culturing Microbiota

text: n

text: m

text: LF/PP


characteristic: External Id

text: CGS-GL

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC secondary accession

text: live

text: Generic

text: n

text: Sample 261-263 from Uncultured Fecal (mouse) sample on Western diet

text: FMA:Feces

text: FMA:Feces

text: USA

text: Western

text: ENVO:feces

text: ENVO:feces

text: ENVO:feces

characteristic: host subject id

characteristic: latitude

characteristic: longitude

text: mouse gut metagenome

text: anaerobe

text: Culturing Microbiota

characteristic: project name

text: n

characteristic: public

text: m

characteristic: sex

text: Western


text: CGS-GL

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

characteristic: SRA accession

text: n

text: Sample 71-73 from Cultured Fecal (mouse) sample on LF/PP diet

text: FMA:Feces

characteristic: body product

text: FMA:Feces

characteristic: body site

text: USA

characteristic: country

characteristic: description

text: LF/PP

text: ENVO:feces

text: ENVO:feces

text: ENVO:feces

text: mouse gut metagenome

text: anaerobe

text: Culturing Microbiota

text: n

text: m

text: LF/PP

characteristic: title


characteristic: External Id

text: CGS-GL

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC secondary accession

text: live

text: Generic

text: n

text: Sample 269-271 from Cultured Fecal (mouse) sample on Western diet

text: FMA:Feces

text: FMA:Feces

text: USA

text: Western

characteristic: diet

characteristic: elevation

text: ENVO:feces

characteristic: env biome

text: ENVO:feces

characteristic: env feature

text: ENVO:feces

characteristic: env matter

text: mouse gut metagenome

characteristic: organism

text: anaerobe

text: Culturing Microbiota

text: n

text: m

text: Western


text: Research Technology Support Facility at Michigan State University

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: endosperm

text: endosperm 8.22

text: standard 454 library

characteristic: library description

text: standard

characteristic: library type

text: Euonymus alatus

text: Euonymus alatus endosperm


text: Snyder, Genetics, Stanford University

text: live

text: Generic

characteristic: SRA accession

text: umbilical vein endothelial cells

characteristic: cell type

text: Homo sapiens

characteristic: passage

text: Chromatin IP against GATA2

characteristic: title


characteristic: External Id

text: Snyder, Genetics, Stanford University

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC secondary accession

text: live

text: Generic

text: umbilical vein endothelial cells

text: Homo sapiens

text: Chromatin IP against cFOS


text: Snyder, Genetics, Stanford University

text: live

text: Generic

text: umbilical vein endothelial cells

text: Homo sapiens

text: control input sample

text: input


text: Harvard University

text: live

text: Generic

text: Rabbit anti Notch1

characteristic: antibody

characteristic: cell line

text: T lymphocyte

text: T lymphoblastic leukemia/lymphoma

characteristic: disease

text: Homo sapiens

text: CUTLL1: Notch1 Chromatin IP


text: Harvard University

characteristic: INSDC last update

text: live

text: Generic

text: Rabbit anti RBPJ

text: T lymphocyte

text: T lymphoblastic leukemia/lymphoma

text: Homo sapiens

characteristic: organism

text: CUTLL1: RBPJ Chromatin IP


text: Harvard University

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: T lymphocyte

text: T lymphoblastic leukemia/lymphoma

text: Homo sapiens

text: CUTLL1: ZNF143 Chromatin IP


text: Harvard University

text: live

text: Generic

characteristic: NCBI submission package

text: T lymphocyte

text: T lymphoblastic leukemia/lymphoma

text: Homo sapiens

text: CUTLL1: input


text: Harvard University

text: live

text: Generic

text: T lymphocyte

text: T lymphoblastic leukemia/lymphoma

text: Homo sapiens

text: CUTLL1: H3K4me1 Chromatin IP


text: Harvard University

text: live

text: Generic

text: T lymphocyte

text: T lymphoblastic leukemia/lymphoma

text: Homo sapiens

text: CUTLL1: H3K4me3 Chromatin IP


text: Harvard University

text: live

text: Generic

text: T lymphocyte

text: T lymphoblastic leukemia/lymphoma

text: Homo sapiens

text: CUTLL1: H3K27me3 Chromatin IP


text: Harvard University

text: live

text: Generic

text: Rabbit anti Notch1

text: T lymphocyte

text: T lymphoblastic leukemia/lymphoma

text: Mus musculus

text: T6E: Notch1 Chromatin IP


text: Harvard University

text: live

text: Generic

text: Rabbit anti RBPJ

text: T lymphocyte

text: T lymphoblastic leukemia/lymphoma

text: Mus musculus

text: T6E: RBPJ Chromatin IP


text: Harvard University

text: live

text: Generic

text: T lymphocyte

text: T lymphoblastic leukemia/lymphoma

text: Mus musculus

text: T6E: Input


text: Harvard University

text: live

text: Generic

text: Rabbit anti Notch1

text: T lymphocyte

text: T lymphoblastic leukemia/lymphoma

text: Mus musculus

text: G4A2: Notch1 Chromatin IP


text: Harvard University

text: live

text: Generic

text: Rabbit anti RBPJ

text: T lymphocyte

text: T lymphoblastic leukemia/lymphoma

text: Mus musculus

text: G4A2: RBPJ Chromatin IP


text: Harvard University

text: live

text: Generic

text: T lymphocyte

text: T lymphoblastic leukemia/lymphoma

text: Mus musculus

text: G4A2: input


text: Nanjing Agricultral University, China

text: live

text: Generic

text: mycelia

characteristic: developmental stage

text: Phytophthora sojae

text: mycelia

characteristic: strain

text: MY


text: Nanjing Agricultral University, China

text: live

text: Generic

characteristic: SRA accession

text: zoosporangia

text: Phytophthora sojae

text: zoosporangia

text: SP


text: Nanjing Agricultral University, China

text: live

text: Generic

text: zoospores

text: Phytophthora sojae

text: zoospores

text: ZO


text: Nanjing Agricultral University, China

text: live

text: Generic

text: cysts

text: Phytophthora sojae

text: cysts

text: CY


text: Nanjing Agricultral University, China

text: live

text: Generic

text: germinating cysts

text: Phytophthora sojae

text: germinating cysts

text: GC


text: Nanjing Agricultral University, China

text: live

text: Generic

text: P.sojae inoculation onto susceptible soybean leaf tissues

characteristic: infection

text: 1.5 h

characteristic: infection time

text: Phytophthora sojae

text: mycelia, 1.5 h after inoculation onto susceptible soybean leaf tissues

text: mycelia

characteristic: tissue


characteristic: External Id

text: Nanjing Agricultral University, China

characteristic: INSDC center name

text: live

text: Generic

text: 3 h

text: Phytophthora sojae

text: mycelia, 3 h after inoculation onto susceptible soybean leaf tissues

text: mycelia

characteristic: title


text: Nanjing Agricultral University, China

characteristic: INSDC first public

characteristic: INSDC secondary accession

text: live

text: Generic

text: 6 h

text: Glycine max

text: mycelia and soybean leaf, 6 h after inoculation onto susceptible soybean leaf tissues

text: mycelia and soybean leaf


text: Nanjing Agricultral University, China

text: live

text: Generic

text: 12 h

text: Glycine max

text: mycelia and soybean leaf, 12 h after inoculation onto susceptible soybean leaf tissues

text: mycelia and soybean leaf


text: Nanjing Agricultral University, China

text: live

text: Generic

text: 24 h

text: Glycine max

text: mycelia and soybean leaf, 24 h after inoculation onto susceptible soybean leaf tissues

text: mycelia and soybean leaf


text: UCLA

text: live

text: Generic

characteristic: cell line

text: glioblastoma

characteristic: cell type

text: Homo sapiens

text: Glioblastoma

text: U87 glioblastoma cell line


text: Cornell University

characteristic: INSDC last update

text: live

text: Generic

text: Mus musculus

characteristic: organism

text: mouse embryonic day 17.5 placental tissue

text: AKR/J mother x PWD/PhJ father

text: placenta

text: Mouse placenta RNAseq AKR/J mother x PWD/PhJ father PA


text: Cornell University

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Mus musculus

text: PWD/PhJ mother x AKR/J father

text: placenta

text: Mouse placenta RNAseq PWD/PhJ mother x AKR/J father AP


Silene vulgaris transcriptome

text: University of Virginia

text: live

text: Generic

characteristic: NCBI submission package

text: Normalized cDNA derived from polyA-selected RNA pool from leaf, root, and floral tissue from 3 genetically divergent populations (ALN1, BOL, and SEE5) of Silene vulgaris.

characteristic: description

text: Silene vulgaris

text: De novo transcriptome assembly and SNP detection in the flowering plant Silene vulgaris (Caryophyllaceae)


ERYEST

text: Agricultural Biotechnology Research Center, Academia Sinica

text: live

text: Generic

characteristic: SRA accession

text: RNAs were isolated from root, leaf, flower and pedicel tissues of Erycina pusilla. Equal amounts of RNAs were pooled for cDNA library construction.

text: Erycina pusilla

text: Transcriptome Database of Erycina pusilla


text: University of California - Davis, Drosophila Population Genomics Project

text: live

text: Generic

text: Sub-Saharan African population - Zambia (Siavonga). Haploid embryo.

text: Drosophila melanogaster


Sample CME_2005_915

text: Center for Comparative Genomics and Bioinformatics

text: live

text: Generic

text: 454 sequencing of MAMMUTHUS PRIMIGENIUS

text: Mammuthus primigenius

text: Sample of MAMMUTHUS PRIMIGENIUS


Sample NUCLEOSOME_H2A.Z

text: live

text: Generic

text: 454 sequencing of SACCHAROMYCES CEREVISIAE

text: Saccharomyces cerevisiae

text: Sample of SACCHAROMYCES CEREVISIAE


text: Joint Genome Institute, U.S. Department of Energy

text: live

text: Generic

text: Agaricus bisporus U1 vegetative mycelium from casing soil

text: vegatative mycelium

text: standard

characteristic: library description

text: standard

characteristic: library type

text: Agaricus bisporus U1

characteristic: strain

text: Generic sample from Agaricus bisporus U1


characteristic: External Id

text: live

text: Generic

text: Agaricus bisporus U1 vegetative mycelium on compost-agar

text: vegatative mycelium

text: standard

text: standard

text: Agaricus bisporus U1


characteristic: INSDC center name

text: live

text: Generic

text: Agaricus bisporus U1 fruitbody (sporophore)

text: reproductive fruiting-body

text: standard

text: standard

text: Agaricus bisporus U1


text: Broad Institute

text: live

text: Generic

text: Blastomyces dermatitidis

text: Blastomyces dermatitidis ATCC18187

characteristic: sample name

text: Generic sample from Ajellomyces dermatitidis

characteristic: title


text: Broad Institute

characteristic: INSDC first public

characteristic: INSDC secondary accession

text: live

text: Generic

text: Generic

characteristic: biosamplemodel

characteristic: collection date

text: Mycobacterium tuberculosis H37Rv

text: Generic sample from Mycobacterium tuberculosis H37Rv


text: Faculty of Biology, Plant Physiology

characteristic: INSDC last update

text: live

text: Generic

text: Agria

characteristic: cultivar

text: Potato tuber buds were cut and ground. cDNA synthesised from RNA extracted from the cut buds.

text: mature tubers

characteristic: dev-stage

text: E.coli TOP10

characteristic: lab-host

text: potato tuber

characteristic: organ

text: Solanum tuberosum

characteristic: organism

text: buds

characteristic: tissue-type

text: Solanum tuberosum Agria (potato) tuber amylases


text: Institute of Himalayan Bioresource Technology

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Leaf

text: Picrorhiza kurrooa

text: Leaf

text: Cloning of cytochrome p450 from Picrorhiza kurrooa Royle ex Benth

text: Plasmid

text: pGEMT-Easy vector (Promega)

characteristic: vector


characteristic: External Id

text: The University of Arizona

characteristic: INSDC center name

text: live

text: Generic

text: DH10B T1 phage resistant

text: Oryza meridionalis

text: HindIII

text: HindIII

text: leaves

text: Plasmid


text: The University of Arizona

characteristic: INSDC first public

text: live

text: Generic

text: DH10B T1 phage resistant

text: Oryza glumipatula

text: HindIII

text: HindIII

text: leaves

characteristic: title

text: Plasmid


text: University of Washington

characteristic: INSDC secondary accession

text: live

text: Generic

characteristic: SRA accession

text: GSC:MIMARKS

characteristic: keywords

text: ice metagenome

text: Multi-year ice samples from two sites near the geographic North Pole


text: University of Washington

characteristic: INSDC last update

text: live

text: Generic

text: Bacteria


Sample M036_454

text: J. Craig Venter Institute

text: live

text: Generic

text: 454 sequencing of BACILLUS SP. NRRL B-14911

characteristic: description

text: Bacillus sp. NRRL B-14911

text: NRRL B-14911

characteristic: strain

text: Sample of BACILLUS SP. NRRL B-14911


Sample M006_454

text: J. Craig Venter Institute

text: live

text: Generic

text: 454 sequencing of ERYTHROBACTER LITORALIS HTCC2594

text: Erythrobacter litoralis HTCC2594

text: Sample of ERYTHROBACTER LITORALIS HTCC2594


Sample M003_454

text: J. Craig Venter Institute

text: live

text: Generic

text: 454 sequencing of JANIBACTER SP. HTCC2649

text: Janibacter sp. HTCC2649

text: Sample of JANIBACTER SP. HTCC2649


Sample M020_454

text: J. Craig Venter Institute

text: live

text: Generic

text: 454 sequencing of PROCHLOROCOCCUS MARINUS STR. MIT 9211

text: Prochlorococcus marinus str. MIT 9211

text: MIT 9211

text: Sample of PROCHLOROCOCCUS MARINUS STR. MIT 9211


Sample M025_454

text: J. Craig Venter Institute

text: live

text: Generic

text: 454 sequencing of PSEUDOALTEROMONAS TUNICATA D2

text: Pseudoalteromonas tunicata D2

text: Sample of PSEUDOALTEROMONAS TUNICATA D2


Sample M063_454

text: J. Craig Venter Institute

text: live

text: Generic

text: 454 sequencing of PSYCHROMONAS SP. CNPT3

text: Psychromonas sp. CNPT3

text: Sample of PSYCHROMONAS SP. CNPT3


VRC0x-like antibody

text: Vaccine Research Center

text: live

text: Generic

text: pt45 HC-Patient 45 Heavy Chain Variable(SRR275668); pt45 kappa-Patient 45 Light Chain Variable(SRR275679); pt74 HC set1- Patient 74 Heavy Chain Variable(SRR275711); pt74 HC set2-Patient 74 Heavy Chain Variable(SRR277211);

text: Homo sapiens

text: 454 sequencing of HIV antibodies


text: University of Missouri

text: live

text: Generic

text: Cultivar/line: Maize inbred B73 Dates grown: late July - early August, 2008 Environment grown in: BPP Field Lab, Corvallis, OR Developmental stage: 9-day old Date of tissue isolation: August 6, 11:30 am; Temp: 22.4°C; Relative humidity 56% Method of tissue isolation: Seedling cut off at first node (includes mature sheath &amp; blade, immature leaves, possibly stem &amp; meristem), placed in liquid nitrogen. Number of individuals pooled in sample: 1 Date of RNA Isolation: 8/25/08 Method of RNA isolation: Tissue ground in liquid nitrogen, processed using Trizol (invitrogen) RNA preparation method. RNEasy MinElute columns (Qiagen) were used to purify total RNA from the aqueous phase from the Trizol prep. Resultant RNA was assessed by Bioanalyzer. Bioanalyzer RNA Concentration &amp; RNA Integrity Number (RIN): SeedlingB or Seedling2 - 1769 ng/ul; 9.9 Date of cDNA preparation: 11/13/08 Method of cDNA preparation: SMART PCR cDNA Synthesis Kit with SMART MMLV Reverse Transcriptase and the Advantage 2 PCR kit (Clontech). cDNA synthesis adapter primers: The 3’ polyA tail for each transcript is used to anneal to the primer for cDNA synthesis from the SMART CDS Primer II A : 5’-AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTVN-3’ ( V=A, C, or G). The 5’ end of each transcript is associated with addition of 3-5 dC residues to the cDNA strand, due to the terminal transferase activity of MMLV-RT. The three dG’s at the 3’ end of the SMART II A oligonucleotide adapter (5’-AAGCAGTGGTATCAACGCAGAGTACGCGGG-3’) anneal to these additional dC’s, and this adapter is used to create the double stranded cDNA via PCR. As per the SMART PCR protocol, an Advantage 2 PCR kit (Clontech) was used to amplify the cDNA for 15 PCR cycles as the final step.

text: Zea mays


text: University of Missouri

text: live

text: Generic

text: Cultivar/line: Maize inbred B73 Dates grown: late July - early August, 2008 Environment grown in: BPP Field Lab, Corvallis, OR Developmental stage: 9-day old Date of tissue isolation: August 6, 11:30 am; Temp: 22.4°C; Relative humidity 56% Method of tissue isolation: Seedling cut off at first node (includes mature sheath &amp; blade, immature leaves, possibly stem &amp; meristem), placed in liquid nitrogen. Number of individuals pooled in sample: 1 Date of RNA Isolation: 8/25/08 Method of RNA isolation: Tissue ground in liquid nitrogen, processed using Trizol (invitrogen) RNA preparation method. RNEasy MinElute columns (Qiagen) were used to purify total RNA from the aqueous phase from the Trizol prep. Resultant RNA was assessed by Bioanalyzer. Bioanalyzer RNA Concentration &amp; RNA Integrity Number (RIN): SeedlingC or Seedling3 - 2264 ng/ul; 10.0 Date of cDNA preparation: 11/3/08 Method of cDNA preparation: SMART PCR cDNA Synthesis Kit with SMART MMLV Reverse Transcriptase and the Advantage 2 PCR kit (Clontech). cDNA synthesis adapter primers: The 3’ polyA tail for each transcript is used to anneal to the primer for cDNA synthesis from the SMART CDS Primer II A : 5’-AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTVN-3’ ( V=A, C, or G). The 5’ end of each transcript is associated with addition of 3-5 dC residues to the cDNA strand, due to the terminal transferase activity of MMLV-RT. The three dG’s at the 3’ end of the SMART II A oligonucleotide adapter (5’-AAGCAGTGGTATCAACGCAGAGTACGCGGG-3’) anneal to these additional dC’s, and this adapter is used to create the double stranded cDNA via PCR. As per the SMART PCR protocol, an Advantage 2 PCR kit (Clontech) was used to amplify the cDNA for 15 PCR cycles as the final step.

text: Zea mays


text: University of Missouri

text: live

text: Generic

text: Cultivar/line: Maize inbred B73 Dates grown: June -August, 2008 Environment grown in: BPP Field Lab, Corvallis, OR Developmental stage: Mature, freshly-shed pollen (post-anther dehiscence) Date of tissue isolation: August 22/23, 11:30 am; Temp: 22.5°C; Relative humidity 56% Method of tissue isolation: Mature pollen harvested using a tassel bag placed over the tassel of a single plant for ~ 1 hour, collected in a cryotube, and placed in liquid nitrogen. Date of RNA Isolation: 8/25/08 Method of RNA isolation: Tissue ground in liquid nitrogen, processed using Trizol (invitrogen) RNA preparation method. RNEasy MinElute columns (Qiagen) were used to purify total RNA from the aqueous phase from the Trizol prep. Resultant RNA was assessed by Bioanalyzer. Bioanalyzer RNA Concentration &amp; RNA Integrity Number (RIN): PollenA or Pollen1 - 438 ng/ul; 9.7 Date of cDNA preparation: 11/13/08 Method of cDNA preparation: SMART PCR cDNA Synthesis Kit with SMART MMLV Reverse Transcriptase and the Advantage 2 PCR kit (Clontech). cDNA synthesis adapter primers: The 3’ polyA tail for each transcript is used to anneal to the primer for cDNA synthesis from the SMART CDS Primer II A : 5’-AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTVN-3’ ( V=A, C, or G). The 5’ end of each transcript is associated with addition of 3-5 dC residues to the cDNA strand, due to the terminal transferase activity of MMLV-RT. The three dG’s at the 3’ end of the SMART II A oligonucleotide adapter (5’-AAGCAGTGGTATCAACGCAGAGTACGCGGG-3’) anneal to these additional dC’s, and this adapter is used to create the double stranded cDNA via PCR. As per the SMART PCR protocol, an Advantage 2 PCR kit (Clontech) was used to amplify the cDNA for 15 PCR cycles as the final step.

text: Zea mays


text: University of Missouri

text: live

text: Generic

text: Cultivar/line: Maize inbred B73 Dates grown: June -August, 2008 Environment grown in: BPP Field Lab, Corvallis, OR Developmental stage: Mature, freshly-shed pollen (post-anther dehiscence) Date of tissue isolation: August 22/23, 11:30 am; Temp: 22.5°C; Relative humidity 56% Method of tissue isolation: Mature pollen harvested using a tassel bag placed over the tassel of a single plant for ~1 hour, collected in a cryotube, and placed in liquid nitrogen. Date of RNA Isolation: 8/25/08 Method of RNA isolation: Tissue ground in liquid nitrogen, processed using Trizol (invitrogen) RNA preparation method. RNEasy MinElute columns (Qiagen) were used to purify total RNA from the aqueous phase from the Trizol prep. Resultant RNA was assessed by Bioanalyzer. Bioanalyzer RNA Concentration &amp; RNA Integrity Number (RIN): PollenB or Pollen2 - 809ng/ul; 9.5 Date of cDNA preparation: 11/3/08 Method of cDNA preparation: SMART PCR cDNA Synthesis Kit with SMART MMLV Reverse Transcriptase and the Advantage 2 PCR kit (Clontech). cDNA synthesis adapter primers: The 3’ polyA tail for each transcript is used to anneal to the primer for cDNA synthesis from the SMART CDS Primer II A : 5’-AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTVN-3’ ( V=A, C, or G). The 5’ end of each transcript is associated with addition of 3-5 dC residues to the cDNA strand, due to the terminal transferase activity of MMLV-RT. The three dG’s at the 3’ end of the SMART II A oligonucleotide adapter (5’-AAGCAGTGGTATCAACGCAGAGTACGCGGG-3’) anneal to these additional dC’s, and this adapter is used to create the double stranded cDNA via PCR. As per the SMART PCR protocol, an Advantage 2 PCR kit (Clontech) was used to amplify the cDNA for 15 PCR cycles as the final step.

text: Zea mays


text: University of Missouri

text: live

text: Generic

text: Cultivar/line: Maize inbred B73 Dates grown: June -August, 2008 Environment grown in: BPP Field Lab, Corvallis, OR Developmental stage: Mature, freshly-shed pollen (post-anther dehiscence) Date of tissue isolation: August 22/23, 11:30 am; Temp: 22.5°C; Relative humidity 56% Method of tissue isolation: Mature pollen harvested using a tassel bag placed over the tassel of a single plant for ~1 hour, collected in a cryotube, and placed in liquid nitrogen. Date of RNA Isolation: 8/25/08 Method of RNA isolation: Tissue ground in liquid nitrogen, processed using Trizol (invitrogen) RNA preparation method. RNEasy MinElute columns (Qiagen) were used to purify total RNA from the aqueous phase from the Trizol prep. Resultant RNA was assessed by Bioanalyzer. Bioanalyzer RNA Concentration &amp; RNA Integrity Number (RIN): PollenC or Pollen3 - 608 ng/ul; 10.0 Date of cDNA preparation: 11/13/08 Method of cDNA preparation: SMART PCR cDNA Synthesis Kit with SMART MMLV Reverse Transcriptase and the Advantage 2 PCR kit (Clontech). cDNA synthesis adapter primers: The 3’ polyA tail for each transcript is used to anneal to the primer for cDNA synthesis from the SMART CDS Primer II A : 5’-AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTVN-3’ ( V=A, C, or G). The 5’ end of each transcript is associated with addition of 3-5 dC residues to the cDNA strand, due to the terminal transferase activity of MMLV-RT. The three dG’s at the 3’ end of the SMART II A oligonucleotide adapter (5’-AAGCAGTGGTATCAACGCAGAGTACGCGGG-3’) anneal to these additional dC’s, and this adapter is used to create the double stranded cDNA via PCR. As per the SMART PCR protocol, an Advantage 2 PCR kit (Clontech) was used to amplify the cDNA for 15 PCR cycles as the final step.

text: Zea mays


text: University of Missouri

text: live

text: Generic

text: Cultivar/line: Maize inbred B73 Dates grown: 2009 Environment grown in: Greenhouse, 16 hr days, 6am sunrise, Palo Alto, CA Developmental stage: Mature ovules and embryo sacs, from ear florets with a silk length of ~10 cm. Time of tissue isolation: 11:00 am. Method of tissue isolation: Based on the methodology of Kranz et al. (1991) and Yang et al. (2006), as described briefly here. The silk and ovary wall were removed with forceps by cutting the ovule from the floret with the edge of the forceps. Each ovule was immediately placed in a Petri dish in a cell wall enzyme digesting mix of 0.75% pectinase, 0.25% pectolyase, 0.5% cellulase, 0.5% hemicellulase buffered in 0.55M mannitol pH 5.0 for one hour after collecting the last ovule at 24 ± 1.0º C. Ovule collection took approximately 1 hour. Embryo sacs (with some attached nucellus cells) were mechanical extracted from the ovules using dissecting needles. The embryo sac samples and remaining ovule tissue (now lacking an embryo sac) were placed in separate microfuge tubes containing 500 µL of 0.55M mannitol pH 5.0. Total embryo sac isolation time was approximately 1 hour. After collecting embryo sacs, and the associated ovule tissue lacking embryo sacs, from 15 to 20 ovules per biological replicate, samples were spun at 3000 rpm for 1 minute. Excess buffer was removed with a pipet tip and 400 µL of Trizol was added to each sample along with a fresh tungsten carbide bead. Samples were vortexed at high speed until ovule tissue pieces were fully ground in the Trizol solution (approximately 2-3 minutes). Tubes were then flash frozen in liquid nitrogen and stored at -80º C, until finishing the RNA extraction protocol in Trizol according to manufacturer’s specifications to isolate total RNA with the addition of 10 µg of RNase-free glycogen for the precipitation as a carrier because of the small sample size. Total RNA isolate (by Nanodrop): Ovule1 - 20,066 ng Method of cDNA preparation: SMARTer PCR cDNA Synthesis Kit with SMART MMLV Reverse Transcriptase and the Advantage 2 PCR kit (Clontech). (NOTE that the SMARTer protocol is optimized for small tissue samples, and slightly modified from the SMART protocol used for the seedling and pollen samples in this study.) cDNA synthesis adapter primers: The 3’ polyA tail for each transcript is used to anneal to the primer for cDNA synthesis from the SMART CDS Primer II A : 5’-AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTVN-3’ ( V=A, C, or G). The 5’ end of each transcript is associated with addition of 3-5 dC residues to the cDNA strand, due to the terminal transferase activity of MMLV-RT. The three dG’s at the 3’ end of the SMART IIer A oligonucleotide adapter (5’-AAGCAGTGGTATCAACGCAGAGTACATGGG-3’) anneal to these additional dC’s, and this adapter is used to create the double stranded cDNA via PCR. As per the SMARTer PCR protocol, an Advantage 2 PCR kit (Clontech) was used to amplify the cDNA for 26 PCR cycles as the final step. The purity of the cDNA sample was assayed by PCR with 5 marker genes: Embryo Sac1 (ES1) (an embryo sac specific gene), Embryo surrounding region1 (ESR1) (an endosperm specific gene), EBE2 (a central cell specific gene), ubiquitin (a constitutive gene), and knox6 (a constitutive gene). This confirmed that the embryo sac/ovule sample pairs had no contaminating post-fertilization seed (endosperm) tissue in either sample, and had no contaminating embryo sac tissue in the ovule sample of the pair.

text: Zea mays


text: University of Missouri

text: live

text: Generic

text: Cultivar/line: Maize inbred B73 Dates grown: 2009 Environment grown in: Greenhouse, 16 hr days, 6am sunrise, Palo Alto, CA Developmental stage: Mature ovules and embryo sacs, from ear florets with a silk length of ~10 cm. Time of tissue isolation: 11:00 am. Method of tissue isolation: Based on the methodology of Kranz et al. (1991) and Yang et al. (2006), as described briefly here. The silk and ovary wall were removed with forceps by cutting the ovule from the floret with the edge of the forceps. Each ovule was immediately placed in a Petri dish in a cell wall enzyme digesting mix of 0.75% pectinase, 0.25% pectolyase, 0.5% cellulase, 0.5% hemicellulase buffered in 0.55M mannitol pH 5.0 for one hour after collecting the last ovule at 24 ± 1.0º C. Ovule collection took approximately 1 hour. Embryo sacs (with some attached nucellus cells) were mechanical extracted from the ovules using dissecting needles. The embryo sac samples and remaining ovule tissue (now lacking an embryo sac) were placed in separate microfuge tubes containing 500 µL of 0.55M mannitol pH 5.0. Total embryo sac isolation time was approximately 1 hour. After collecting embryo sacs, and the associated ovule tissue lacking embryo sacs, from 15 to 20 ovules per biological replicate, samples were spun at 3000 rpm for 1 minute. Excess buffer was removed with a pipet tip and 400 µL of Trizol was added to each sample along with a fresh tungsten carbide bead. Samples were vortexed at high speed until ovule tissue pieces were fully ground in the Trizol solution (approximately 2-3 minutes). Tubes were then flash frozen in liquid nitrogen and stored at -80º C, until finishing the RNA extraction protocol in Trizol according to manufacturer’s specifications to isolate total RNA with the addition of 10 µg of RNase-free glycogen for the precipitation as a carrier because of the small sample size. Total RNA isolate (by Nanodrop): Ovule2 - 11,850 ng Method of cDNA preparation: SMARTer PCR cDNA Synthesis Kit with SMART MMLV Reverse Transcriptase and the Advantage 2 PCR kit (Clontech). (NOTE that the SMARTer protocol is optimized for small tissue samples, and slightly modified from the SMART protocol used for the seedling and pollen samples in this study.) cDNA synthesis adapter primers: The 3’ polyA tail for each transcript is used to anneal to the primer for cDNA synthesis from the SMART CDS Primer II A : 5’-AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTVN-3’ ( V=A, C, or G). The 5’ end of each transcript is associated with addition of 3-5 dC residues to the cDNA strand, due to the terminal transferase activity of MMLV-RT. The three dG’s at the 3’ end of the SMART IIer A oligonucleotide adapter (5’-AAGCAGTGGTATCAACGCAGAGTACATGGG-3’) anneal to these additional dC’s, and this adapter is used to create the double stranded cDNA via PCR. As per the SMARTer PCR protocol, an Advantage 2 PCR kit (Clontech) was used to amplify the cDNA for 26 PCR cycles as the final step. The purity of the cDNA sample was assayed by PCR with 5 marker genes: Embryo Sac1 (ES1) (an embryo sac specific gene), Embryo surrounding region1 (ESR1) (an endosperm specific gene), EBE2 (a central cell specific gene), ubiquitin (a constitutive gene), and knox6 (a constitutive gene). This confirmed that the embryo sac/ovule sample pairs had no contaminating post-fertilization seed (endosperm) tissue in either sample, and had no contaminating embryo sac tissue in the ovule sample of the pair.

text: Zea mays


text: University of Missouri

text: live

text: Generic

text: Cultivar/line: Maize inbred B73 Dates grown: 2009 Environment grown in: Greenhouse, 16 hr days, 6am sunrise, Palo Alto, CA Developmental stage: Mature ovules and embryo sacs, from ear florets with a silk length of ~10 cm. Time of tissue isolation: 11:00 am. Method of tissue isolation: Based on the methodology of Kranz et al. (1991) and Yang et al. (2006), as described briefly here. The silk and ovary wall were removed with forceps by cutting the ovule from the floret with the edge of the forceps. Each ovule was immediately placed in a Petri dish in a cell wall enzyme digesting mix of 0.75% pectinase, 0.25% pectolyase, 0.5% cellulase, 0.5% hemicellulase buffered in 0.55M mannitol pH 5.0 for one hour after collecting the last ovule at 24 ± 1.0º C. Ovule collection took approximately 1 hour. Embryo sacs (with some attached nucellus cells) were mechanical extracted from the ovules using dissecting needles. The embryo sac samples and remaining ovule tissue (now lacking an embryo sac) were placed in separate microfuge tubes containing 500 µL of 0.55M mannitol pH 5.0. Total embryo sac isolation time was approximately 1 hour. After collecting embryo sacs, and the associated ovule tissue lacking embryo sacs, from 15 to 20 ovules per biological replicate, samples were spun at 3000 rpm for 1 minute. Excess buffer was removed with a pipet tip and 400 µL of Trizol was added to each sample along with a fresh tungsten carbide bead. Samples were vortexed at high speed until ovule tissue pieces were fully ground in the Trizol solution (approximately 2-3 minutes). Tubes were then flash frozen in liquid nitrogen and stored at -80º C, until finishing the RNA extraction protocol in Trizol according to manufacturer’s specifications to isolate total RNA with the addition of 10 µg of RNase-free glycogen for the precipitation as a carrier because of the small sample size. Total RNA isolate (by Nanodrop): Ovule3 - 19,516 ng Method of cDNA preparation: SMARTer PCR cDNA Synthesis Kit with SMART MMLV Reverse Transcriptase and the Advantage 2 PCR kit (Clontech). (NOTE that the SMARTer protocol is optimized for small tissue samples, and slightly modified from the SMART protocol used for the seedling and pollen samples in this study.) cDNA synthesis adapter primers: The 3’ polyA tail for each transcript is used to anneal to the primer for cDNA synthesis from the SMART CDS Primer II A : 5’-AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTVN-3’ ( V=A, C, or G). The 5’ end of each transcript is associated with addition of 3-5 dC residues to the cDNA strand, due to the terminal transferase activity of MMLV-RT. The three dG’s at the 3’ end of the SMART IIer A oligonucleotide adapter (5’-AAGCAGTGGTATCAACGCAGAGTACATGGG-3’) anneal to these additional dC’s, and this adapter is used to create the double stranded cDNA via PCR. As per the SMARTer PCR protocol, an Advantage 2 PCR kit (Clontech) was used to amplify the cDNA for 26 PCR cycles as the final step. The purity of the cDNA sample was assayed by PCR with 5 marker genes: Embryo Sac1 (ES1) (an embryo sac specific gene), Embryo surrounding region1 (ESR1) (an endosperm specific gene), EBE2 (a central cell specific gene), ubiquitin (a constitutive gene), and knox6 (a constitutive gene). This confirmed that the embryo sac/ovule sample pairs had no contaminating post-fertilization seed (endosperm) tissue in either sample, and had no contaminating embryo sac tissue in the ovule sample of the pair.

text: Zea mays


text: University of Missouri

text: live

text: Generic

text: Cultivar/line: Maize inbred B73 Dates grown: 2009 Environment grown in: Greenhouse, 16 hr days, 6am sunrise, Palo Alto, CA Developmental stage: Mature ovules and embryo sacs, from ear florets with a silk length of ~10 cm. Time of tissue isolation: 11:00 am. Method of tissue isolation: Based on the methodology of Kranz et al. (1991) and Yang et al. (2006), as described briefly here. The silk and ovary wall were removed with forceps by cutting the ovule from the floret with the edge of the forceps. Each ovule was immediately placed in a Petri dish in a cell wall enzyme digesting mix of 0.75% pectinase, 0.25% pectolyase, 0.5% cellulase, 0.5% hemicellulase buffered in 0.55M mannitol pH 5.0 for one hour after collecting the last ovule at 24 ± 1.0º C. Ovule collection took approximately 1 hour. Embryo sacs (with some attached nucellus cells) were mechanical extracted from the ovules using dissecting needles. The embryo sac samples and remaining ovule tissue (now lacking an embryo sac) were placed in separate microfuge tubes containing 500 µL of 0.55M mannitol pH 5.0. Total embryo sac isolation time was approximately 1 hour. After collecting embryo sacs, and the associated ovule tissue lacking embryo sacs, from 15 to 20 ovules per biological replicate, samples were spun at 3000 rpm for 1 minute. Excess buffer was removed with a pipet tip and 400 µL of Trizol was added to each sample along with a fresh tungsten carbide bead. Samples were vortexed at high speed until ovule tissue pieces were fully ground in the Trizol solution (approximately 2-3 minutes). Tubes were then flash frozen in liquid nitrogen and stored at -80º C, until finishing the RNA extraction protocol in Trizol according to manufacturer’s specifications to isolate total RNA with the addition of 10 µg of RNase-free glycogen for the precipitation as a carrier because of the small sample size. Total RNA isolate (by Nanodrop): EmbryoSac1 - 1215 ng Method of cDNA preparation: SMARTer PCR cDNA Synthesis Kit with SMART MMLV Reverse Transcriptase and the Advantage 2 PCR kit (Clontech). (NOTE that the SMARTer protocol is optimized for small tissue samples, and slightly modified from the SMART protocol used for the seedling and pollen samples in this study.) cDNA synthesis adapter primers: The 3’ polyA tail for each transcript is used to anneal to the primer for cDNA synthesis from the SMART CDS Primer II A : 5’-AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTVN-3’ ( V=A, C, or G). The 5’ end of each transcript is associated with addition of 3-5 dC residues to the cDNA strand, due to the terminal transferase activity of MMLV-RT. The three dG’s at the 3’ end of the SMART IIer A oligonucleotide adapter (5’-AAGCAGTGGTATCAACGCAGAGTACATGGG-3’) anneal to these additional dC’s, and this adapter is used to create the double stranded cDNA via PCR. As per the SMARTer PCR protocol, an Advantage 2 PCR kit (Clontech) was used to amplify the cDNA for 26 PCR cycles as the final step. The purity of the cDNA sample was assayed by PCR with 5 marker genes: Embryo Sac1 (ES1) (an embryo sac specific gene), Embryo surrounding region1 (ESR1) (an endosperm specific gene), EBE2 (a central cell specific gene), ubiquitin (a constitutive gene), and knox6 (a constitutive gene). This confirmed that the embryo sac/ovule sample pairs had no contaminating post-fertilization seed (endosperm) tissue in either sample, and had no contaminating embryo sac tissue in the ovule sample of the pair.

text: Zea mays


text: University of Missouri

text: live

text: Generic

text: Cultivar/line: Maize inbred B73 Dates grown: 2009 Environment grown in: Greenhouse, 16 hr days, 6am sunrise, Palo Alto, CA Developmental stage: Mature ovules and embryo sacs, from ear florets with a silk length of ~10 cm. Time of tissue isolation: 11:00 am. Method of tissue isolation: Based on the methodology of Kranz et al. (1991) and Yang et al. (2006), as described briefly here. The silk and ovary wall were removed with forceps by cutting the ovule from the floret with the edge of the forceps. Each ovule was immediately placed in a Petri dish in a cell wall enzyme digesting mix of 0.75% pectinase, 0.25% pectolyase, 0.5% cellulase, 0.5% hemicellulase buffered in 0.55M mannitol pH 5.0 for one hour after collecting the last ovule at 24 ± 1.0º C. Ovule collection took approximately 1 hour. Embryo sacs (with some attached nucellus cells) were mechanical extracted from the ovules using dissecting needles. The embryo sac samples and remaining ovule tissue (now lacking an embryo sac) were placed in separate microfuge tubes containing 500 µL of 0.55M mannitol pH 5.0. Total embryo sac isolation time was approximately 1 hour. After collecting embryo sacs, and the associated ovule tissue lacking embryo sacs, from 15 to 20 ovules per biological replicate, samples were spun at 3000 rpm for 1 minute. Excess buffer was removed with a pipet tip and 400 µL of Trizol was added to each sample along with a fresh tungsten carbide bead. Samples were vortexed at high speed until ovule tissue pieces were fully ground in the Trizol solution (approximately 2-3 minutes). Tubes were then flash frozen in liquid nitrogen and stored at -80º C, until finishing the RNA extraction protocol in Trizol according to manufacturer’s specifications to isolate total RNA with the addition of 10 µg of RNase-free glycogen for the precipitation as a carrier because of the small sample size. Total RNA isolate (by Nanodrop): EmbryoSac2 - 988 ng Method of cDNA preparation: SMARTer PCR cDNA Synthesis Kit with SMART MMLV Reverse Transcriptase and the Advantage 2 PCR kit (Clontech). (NOTE that the SMARTer protocol is optimized for small tissue samples, and slightly modified from the SMART protocol used for the seedling and pollen samples in this study.) cDNA synthesis adapter primers: The 3’ polyA tail for each transcript is used to anneal to the primer for cDNA synthesis from the SMART CDS Primer II A : 5’-AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTVN-3’ ( V=A, C, or G). The 5’ end of each transcript is associated with addition of 3-5 dC residues to the cDNA strand, due to the terminal transferase activity of MMLV-RT. The three dG’s at the 3’ end of the SMART IIer A oligonucleotide adapter (5’-AAGCAGTGGTATCAACGCAGAGTACATGGG-3’) anneal to these additional dC’s, and this adapter is used to create the double stranded cDNA via PCR. As per the SMARTer PCR protocol, an Advantage 2 PCR kit (Clontech) was used to amplify the cDNA for 26 PCR cycles as the final step. The purity of the cDNA sample was assayed by PCR with 5 marker genes: Embryo Sac1 (ES1) (an embryo sac specific gene), Embryo surrounding region1 (ESR1) (an endosperm specific gene), EBE2 (a central cell specific gene), ubiquitin (a constitutive gene), and knox6 (a constitutive gene). This confirmed that the embryo sac/ovule sample pairs had no contaminating post-fertilization seed (endosperm) tissue in either sample, and had no contaminating embryo sac tissue in the ovule sample of the pair.

text: Zea mays


text: University of Missouri

text: live

text: Generic

text: Cultivar/line: Maize inbred B73 Dates grown: 2009 Environment grown in: Greenhouse, 16 hr days, 6am sunrise, Palo Alto, CA Developmental stage: Mature ovules and embryo sacs, from ear florets with a silk length of ~10 cm. Time of tissue isolation: 11:00 am. Method of tissue isolation: Based on the methodology of Kranz et al. (1991) and Yang et al. (2006), as described briefly here. The silk and ovary wall were removed with forceps by cutting the ovule from the floret with the edge of the forceps. Each ovule was immediately placed in a Petri dish in a cell wall enzyme digesting mix of 0.75% pectinase, 0.25% pectolyase, 0.5% cellulase, 0.5% hemicellulase buffered in 0.55M mannitol pH 5.0 for one hour after collecting the last ovule at 24 ± 1.0º C. Ovule collection took approximately 1 hour. Embryo sacs (with some attached nucellus cells) were mechanical extracted from the ovules using dissecting needles. The embryo sac samples and remaining ovule tissue (now lacking an embryo sac) were placed in separate microfuge tubes containing 500 µL of 0.55M mannitol pH 5.0. Total embryo sac isolation time was approximately 1 hour. After collecting embryo sacs, and the associated ovule tissue lacking embryo sacs, from 15 to 20 ovules per biological replicate, samples were spun at 3000 rpm for 1 minute. Excess buffer was removed with a pipet tip and 400 µL of Trizol was added to each sample along with a fresh tungsten carbide bead. Samples were vortexed at high speed until ovule tissue pieces were fully ground in the Trizol solution (approximately 2-3 minutes). Tubes were then flash frozen in liquid nitrogen and stored at -80º C, until finishing the RNA extraction protocol in Trizol according to manufacturer’s specifications to isolate total RNA with the addition of 10 µg of RNase-free glycogen for the precipitation as a carrier because of the small sample size. Total RNA isolate (by Nanodrop): EmbryoSac3 - 416 ng Method of cDNA preparation: SMARTer PCR cDNA Synthesis Kit with SMART MMLV Reverse Transcriptase and the Advantage 2 PCR kit (Clontech). (NOTE that the SMARTer protocol is optimized for small tissue samples, and slightly modified from the SMART protocol used for the seedling and pollen samples in this study.) cDNA synthesis adapter primers: The 3’ polyA tail for each transcript is used to anneal to the primer for cDNA synthesis from the SMART CDS Primer II A : 5’-AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTVN-3’ ( V=A, C, or G). The 5’ end of each transcript is associated with addition of 3-5 dC residues to the cDNA strand, due to the terminal transferase activity of MMLV-RT. The three dG’s at the 3’ end of the SMART IIer A oligonucleotide adapter (5’-AAGCAGTGGTATCAACGCAGAGTACATGGG-3’) anneal to these additional dC’s, and this adapter is used to create the double stranded cDNA via PCR. As per the SMARTer PCR protocol, an Advantage 2 PCR kit (Clontech) was used to amplify the cDNA for 26 PCR cycles as the final step. The purity of the cDNA sample was assayed by PCR with 5 marker genes: Embryo Sac1 (ES1) (an embryo sac specific gene), Embryo surrounding region1 (ESR1) (an endosperm specific gene), EBE2 (a central cell specific gene), ubiquitin (a constitutive gene), and knox6 (a constitutive gene). This confirmed that the embryo sac/ovule sample pairs had no contaminating post-fertilization seed (endosperm) tissue in either sample, and had no contaminating embryo sac tissue in the ovule sample of the pair.

text: Zea mays


text: Reproductive Biology, Animal Biology, University of Pennsylvania

text: live

text: Generic

text: Thy1+ spermatogonial stem cell

characteristic: cell type

text: Thy1.2 MACS Antibody

characteristic: macs antibody

text: Mus musculus

text: Testis

characteristic: source tissue

text: Freshly isolated SSC enriched testis population

text: Thy1+ SSC-enriched rep1


text: live

characteristic: INSDC status

text: Generic

text: Thy1+ spermatogonial stem cell

text: Thy1.2 MACS Antibody

text: Mus musculus

characteristic: organism

text: Testis

text: Thy1+ SSC-enriched rep2


text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Thy1- spermatogonial stem cell

text: Thy1.2 MACS Antibody

text: Mus musculus

text: Testis

text: Freshly isolated SSC depleted testis population

text: Thy1- Somatic-enriched rep1


characteristic: External Id

text: live

text: Generic

text: Thy1- spermatogonial stem cell

text: Thy1.2 MACS Antibody

text: Mus musculus

text: Testis

text: Thy1- Somatic-enriched rep2


characteristic: INSDC center name

text: live

text: Generic

text: spermatogonial stem cell enriched cell culture

text: Thy1.2 MACS Antibody

text: Mus musculus

characteristic: passages

text: Testis

text: SSC enriched germ cell culture derived from Thy1+ SSC-enriched cell population

text: SSC-enriched germ cell culture 1


text: Palczewski, Pharmacology, Case Western Reserve University

characteristic: INSDC first public

text: live

text: Generic

text: Wild type

characteristic: genotype/variation

text: Mus musculus

text: Eye tissue

text: Whole Eye

characteristic: tissue

characteristic: title


characteristic: INSDC last update

characteristic: INSDC secondary accession

text: live

text: Generic

characteristic: SRA accession

text: Nrl gene knockout

text: Mus musculus

text: Eye tissue

text: Whole Eye


text: live

text: Generic

text: Wild type

text: Mus musculus

text: Retina tissue

characteristic: strain

text: Retina


text: live

text: Generic

text: Nrl gene knockout

text: Mus musculus

text: Retina tissue

text: Retina


text: Institute for Genome Sciences & Policy, Duke University

text: live

text: Generic

text: Flag M2

characteristic: antibody

text: 4-SU / 365 nm

characteristic: crosslinker and crosslinking wavelength

text: HuR, ELAVL1

characteristic: immunoprecipitated protein

text: Homo sapiens

text: HEK 293

text: HuR PAR-CLIP


name: PRJNA28331.Morganella morganii AC2_8_11 AN D6 FAA 4

text: Broad Institute

text: live

characteristic: INSDC status

text: Generic

characteristic: organism

characteristic: sample name

text: AC2_8_11 AN FAA D6 4

text: Generic sample from Morganella morganii AC2_8_11_AN_D6_FAA_4


characteristic: External Id

text: Broad Institute

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Generic sample from Lachnospiraceae bacterium ORNL_2UT_Ab5152_172


PRJNA28331.Proteus mirabilis WGLW4

text: Broad Institute

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: Proteus mirabilis WGLW4

text: Proteus mirabilis WGLW4

text: Generic sample from Proteus mirabilis WGLW4


name: 123134.5087.New Tech Library.SAIAB 97564

text: Broad Institute

text: live

text: Generic

text: Latimeria chalumnae

text: Latimeria chalumnae HMW DNA 6/1/10

characteristic: sample id

text: New Tech Library

characteristic: sample type

text: Wild caught

text: Coelacanth, South African Wild caught New Tech Library sample SAIAB 97564


text: University of Waterloo

characteristic: INSDC last update

text: live

text: Generic

text: Library of bacterial 16S rRNA genes sampled from piezometer Z103 at Zorra site, sampled at 7.5m below ground surface. Approximately 60 meters away from manure lagoon (contamination source)

characteristic: description

text: groundwater metagenome

characteristic: title


text: University of Waterloo

characteristic: INSDC secondary accession

text: live

text: Generic

characteristic: SRA accession

text: Library of bacterial 16S rRNA genes sampled from piezometer Z106 at Zorra site, sampled at 7.5m below ground surface. Approximately 10 meters away from manure lagoon (contamination source)

text: groundwater metagenome


text: Insect Sciences, Zhejiang University

text: live

text: Generic

characteristic: cultivar

text: mock

characteristic: inocula

text: Nicotiana tabacum

text: Tobacco leaves

text: leaves

characteristic: tissue

text: Tobacco_Mock inoculated


text: Insect Sciences, Zhejiang University

text: live

characteristic: INSDC status

text: Generic

text: TYLCCNV

text: Nicotiana tabacum

characteristic: organism

text: Tobacco leaves

text: leaves

text: Tobacco_TYLCCNV infected


characteristic: External Id

text: Insect Sciences, Zhejiang University

text: live

text: Generic

text: Nicotiana tabacum

text: Tobacco leaves

text: whitefly infested

characteristic: stess

text: leaves

text: Tobacco_Whitefly infested


text: Insect Sciences, Zhejiang University

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: TYLCCNV

text: Nicotiana tabacum

text: Tobacco leaves

text: whitefly infested

text: leaves

text: Tobacco_TYLCCNV and whitefly co-infested


text: dbGaP

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: high molecular weight genomic DNA

characteristic: analyte type

characteristic: biospecimen repository

characteristic: biospecimen repository sample id

characteristic: body site

text: DS-CA-PUB-MDS

text: chronic lymphocytic leukemia

characteristic: histological type

text: Yes

characteristic: is tumor

text: SNP Genotypes (Array)

characteristic: molecular data type

text: Homo sapiens

text: female

characteristic: sex

text: Tumor vs. Matched-Normal

characteristic: study design

characteristic: study name

characteristic: submitted sample id

characteristic: submitted subject id

characteristic: submitter handle

text: Tumor high molecular weight genomic DNA sample from peripheral blood mononuclear CD5/CD19 positive cells of a human female participant in the dbGaP study Genome-Wide Analysis of Chronic Lymphocytic Leukemia


characteristic: External Id

text: dbGaP

characteristic: INSDC last update

characteristic: INSDC secondary accession

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: SRA accession

text: high molecular weight genomic DNA

text: peripheral blood granulocytes

text: DS-CA-PUB-MDS

text: not applicable (if normal DNA)

text: No

text: SNP Genotypes (Array)

text: Homo sapiens

characteristic: organism

text: female

text: Tumor vs. Matched-Normal

text: Non-tumor high molecular weight genomic DNA sample from peripheral blood granulocytes of a human female participant in the dbGaP study Genome-Wide Analysis of Chronic Lymphocytic Leukemia

characteristic: title


text: dbGaP

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

characteristic: NCBI submission package

text: high molecular weight genomic DNA

characteristic: analyte type

characteristic: biospecimen repository

characteristic: biospecimen repository sample id

text: peripheral blood mononuclear CD5/CD19 positive cells

characteristic: body site

text: DS-CA-PUB-MDS

text: chronic lymphocytic leukemia

characteristic: histological type

text: Yes

text: SNP Genotypes (Array)

text: Homo sapiens

text: female

text: Tumor vs. Matched-Normal

text: Genome-Wide Analysis of Chronic Lymphocytic Leukemia


text: dbGaP

text: live

text: Generic

text: high molecular weight genomic DNA

text: peripheral blood granulocytes

text: DS-CA-PUB-MDS

text: not applicable (if normal DNA)

text: No

characteristic: is tumor

text: SNP Genotypes (Array)

characteristic: molecular data type

text: Homo sapiens

text: male

characteristic: sex

text: Tumor vs. Matched-Normal

characteristic: study design

characteristic: study name

characteristic: submitted sample id

characteristic: submitted subject id

characteristic: submitter handle

text: Non-tumor high molecular weight genomic DNA sample from peripheral blood granulocytes of a human male participant in the dbGaP study Genome-Wide Analysis of Chronic Lymphocytic Leukemia


characteristic: External Id

text: dbGaP

characteristic: INSDC last update

characteristic: INSDC secondary accession

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: SRA accession

text: high molecular weight genomic DNA

text: DS-CA-PUB-MDS

text: chronic lymphocytic leukemia

text: Yes

text: SNP Genotypes (Array)

text: Homo sapiens

characteristic: organism

text: male

text: Tumor vs. Matched-Normal

text: Tumor high molecular weight genomic DNA sample from peripheral blood mononuclear CD5/CD19 positive cells of a human male participant in the dbGaP study Genome-Wide Analysis of Chronic Lymphocytic Leukemia

characteristic: title


text: dbGaP

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

characteristic: NCBI submission package

text: high molecular weight genomic DNA

characteristic: analyte type

characteristic: biospecimen repository

characteristic: biospecimen repository sample id

text: peripheral blood granulocytes

characteristic: body site

text: DS-CA-PUB-MDS

text: not applicable (if normal DNA)

characteristic: histological type

text: No

text: SNP Genotypes (Array)

text: Homo sapiens

text: female

text: Tumor vs. Matched-Normal


text: Bielefeld University

text: live

text: Generic

text: Arabidopsis thaliana

text: Arabidopsis thaliana T-DNA insertion lines (Weisshaar 2011)


text: North-East Institute of Science & Technology (CSIR)

text: live

text: Generic

text: Lycopersicon esculentum

characteristic: lab-host

text: Fusarium oxysporum

characteristic: strain

text: Fusarium oxysporum F1SCAR genomic library


default_uncultured glomeromycota

characteristic: External Id

text: MPG Operations

characteristic: INSDC last update

text: live

text: Generic

text: LSU sequences obtained through PCR amplification with FLR3 and FLR4 primers from a complex mixture of AMF species in native plant roots

characteristic: description

text: uncultured Glomeromycotina

text: AMF community from native plant roots at MPG study site.


text: Brigham Young University

characteristic: INSDC secondary accession

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Fungal cultures and tissue were prepared as described by Boose et al. (2011). DNA was isolated from mycelium of the CCB06 isolate of P. semeniperda, collected from a site at Cinder Cone Butte, ID, using the ZR Fungal/Bacterial DNA MiniPrepTM from Zymo Research (Zymo Research Corporation, Orange, CA) following the manufacturer's protocol. DNA was quantified using the NanoDrop ND-1000 spectrophotometer (NanoDrop products, Wilmington, DE). Boose, D., Harrison, S., Clement, S. & Meyer, S. (2011). Population genetic structure of the seed pathogen Pyrenophora semeniperda on Bromus tectorum in western North America. Mycologia 103, 85-93.

characteristic: isolate

text: Pyrenophora seminiperda

text: 454 Sequencing of Pyrenophora semeniperda (genomic library)


text: Brigham Young University

characteristic: INSDC center name

text: live

text: Generic

characteristic: SRA accession

text: Fungal cultures and tissue were prepared as described by Boose et al.(2011). RNA was isolated from two P. semeniperda isolates using the ZR Fungal/Bacterial RNA MiniPrepTM from Zymo Research (Zymo Research Corporation, Orange, CA) and stored at -80* C;RNA was collected from multiple tissue types including mycelium, fingers, and conidia from P.semeniperda isolates including CCB06 and the WRK0 isolate, collected from a site at Skull Valley,UT. RNA quality and integrity was assessed for each extraction using the RNA Nano 6000 kit from Agilent Technologies and the 2100 Bioanalyzer Expert software (Agilent Technologies, Santa Clara,CA). RNA quantity was measured with the TBS-380 Mini-Fluorometer (Turner Biosystems,Sunnyvale, CA) in combination with the RiboGreen RNA quantitation reagent (Molecular Probes,Eugene, OR). Boose, D., Harrison, S., Clement, S. & Meyer, S. (2011). Population genetic structure of the seed pathogen Pyrenophora semeniperda on Bromus tectorum in western North America. Mycologia 103, 85-93.

text: Pyrenophora seminiperda

characteristic: organism

text: 454 Sequencing of Pyrenophora semeniperda (cDNA library)

characteristic: title


text: Brigham Young University

characteristic: INSDC first public

text: live

text: Generic

characteristic: NCBI submission package

text: Pyrenophora seminiperda

text: 454 Sequencing of Pyrenophora semeniperda (3 kb paired-end library - I)


text: Brigham Young University

text: live

text: Generic

text: Pyrenophora seminiperda

text: 454 Sequencing of Pyrenophora semeniperda (3 kb paired-end library - II)


name: Transcriptome of STZ induced diabetic mice MII stage oocytes

text: Institute of Zoology Chinese Academy of Sciences

text: live

text: Generic

text: Mus musculus


Chickpea (PI489777)

text: National Institute of Plant Genome Research (NIPGR)

text: live

text: Generic

text: Shoot tissue sample of chickpea (PI489777) was used for transcriptome sequencing on Roche 454 platform

text: Cicer arietinum

text: Shoots

characteristic: tissue

text: Chickpea (PI489777) transcriptome pyrosequencing - Shoots


name: Transcriptome of STZ control mice MII stage oocytes

text: live

text: Generic

text: Mus musculus

text: Transcriptome of STZ control ICR mice MII stage oocytes


name: Transcriptome of NOD diabetic mice MII stage oocytes

text: live

text: Generic

text: Mus musculus


name: Transcriptome of NOD control mice MII stage oocytes

text: live

text: Generic

text: Mus musculus


Lens culinaris --Precoz

text: National Institure of Plant Genome Research

text: live

text: Generic

text: Lens culinaris

text: Lens culinaris - cultivar- Precoz


Sample FFFY

text: Joint Genome Institute, U.S. Department of Energy

text: live

text: Generic

text: 454 sequencing of ASPERGILLUS NIGER ATCC 1015

text: Aspergillus niger ATCC 1015

text: Sample of ASPERGILLUS NIGER ATCC 1015


Sample FFFG

text: live

text: Generic

text: 454 sequencing of BACILLUS WEIHENSTEPHANENSIS KBAB4

text: Bacillus mycoides KBAB4

text: Sample of BACILLUS WEIHENSTEPHANENSIS KBAB4


Sample FBFF

text: live

text: Generic

text: 454 sequencing of BURKHOLDERIA MULTIVORANS ATCC 17616

text: Burkholderia multivorans ATCC 17616

text: ATCC 17616

text: Sample of BURKHOLDERIA MULTIVORANS ATCC 17616


Sample FBGH

text: live

text: Generic

text: 454 sequencing of CALDICELLULOSIRUPTOR SACCHAROLYTICUS DSM 8903

text: Caldicellulosiruptor saccharolyticus DSM 8903

text: DSM 8903

text: Sample of CALDICELLULOSIRUPTOR SACCHAROLYTICUS DSM 8903


Sample FBHF

text: live

text: Generic

text: 454 sequencing of CALDIVIRGA MAQUILINGENSIS IC-167

text: Caldivirga maquilingensis IC-167

text: Sample of CALDIVIRGA MAQUILINGENSIS IC-167


Sample FBFH

text: live

text: Generic

text: 454 sequencing of CHLOROFLEXUS AGGREGANS DSM 9485

text: Chloroflexus aggregans DSM 9485

text: DSM 9485

text: Sample of CHLOROFLEXUS AGGREGANS DSM 9485


Sample FAHW

text: live

text: Generic

text: 454 sequencing of CLOSTRIDIUM CELLULOLYTICUM H10

text: Ruminiclostridium cellulolyticum H10

text: Sample of CLOSTRIDIUM CELLULOLYTICUM H10


Sample FBGP

text: live

text: Generic

text: 454 sequencing of CLOSTRIDIUM PHYTOFERMENTANS ISDG

text: Lachnoclostridium phytofermentans ISDg

text: ISDg

text: Sample of CLOSTRIDIUM PHYTOFERMENTANS ISDG


Sample FBFY

text: Joint Genome Institute, U.S. Department of Energy

text: live

text: Generic

text: 454 sequencing of CLOSTRIDIUM SP. OHILAS

text: Alkaliphilus oremlandii OhILAs

text: OhILAs

text: Sample of CLOSTRIDIUM SP. OHILAS


Sample FBFI

text: live

text: Generic

text: 454 sequencing of COMAMONAS TESTOSTERONI KF-1

text: Comamonas testosteroni KF-1

text: Sample of COMAMONAS TESTOSTERONI KF-1


Sample FBFN

text: live

text: Generic

text: 454 sequencing of DELFTIA ACIDOVORANS SPH-1

text: Delftia acidovorans SPH-1

text: Sample of DELFTIA ACIDOVORANS SPH-1


Sample FBCZ

text: live

text: Generic

text: Neanderthal fossil microbial communities from Vindija Cave, Croatia

text: fossil metagenome


Sample FBFU

text: live

text: Generic

text: 454 sequencing of FERVIDOBACTERIUM NODOSUM RT17-B1

text: Fervidobacterium nodosum Rt17-B1

text: Sample of FERVIDOBACTERIUM NODOSUM RT17-B1


Sample FBGN

text: live

text: Generic

text: 454 sequencing of HALORHODOSPIRA HALOPHILA SL1

text: Halorhodospira halophila SL1

text: Sample of HALORHODOSPIRA HALOPHILA SL1


Sample FBGT

text: live

text: Generic

text: 454 sequencing of HALOTHERMOTHRIX ORENII H 168

text: Halothermothrix orenii H 168

text: H 168

text: Sample of HALOTHERMOTHRIX ORENII H 168


Sample FBFX

text: live

text: Generic

text: 454 sequencing of HERPETOSIPHON AURANTIACUS ATCC 23779

text: Herpetosiphon aurantiacus DSM 785

text: DSM 785

text: Sample of HERPETOSIPHON AURANTIACUS ATCC 23779


Sample FBHA

text: live

text: Generic

text: 454 sequencing of LACTOBACILLUS REUTERI 100-23

text: rodentium

characteristic: sub species

text: Sample of LACTOBACILLUS REUTERI 100-23

text: type strain of Limosilactobacillus reuteri subsp. rodentium

characteristic: type material


Sample FBHH

text: live

text: Generic

text: 454 sequencing of METHANOCOCCUS AEOLICUS NANKAI-3

text: Methanococcus aeolicus Nankai-3

text: Sample of METHANOCOCCUS AEOLICUS NANKAI-3


Sample FBHC

text: live

text: Generic

text: 454 sequencing of METHANOCORPUSCULUM LABREANUM Z

text: Methanocorpusculum labreanum Z

text: Sample of METHANOCORPUSCULUM LABREANUM Z


Sample FGBS

text: Joint Genome Institute, U.S. Department of Energy

text: live

text: Generic

text: 454 sequencing of METHYLOBACTERIUM SP. 4-46

text: Methylobacterium sp. 4-46

characteristic: strain

text: Sample of METHYLOBACTERIUM SP. 4-46


Sample FBHU

characteristic: External Id

text: live

text: Generic

text: 454 sequencing of MIMULUS GUTTATUS

text: Erythranthe guttata

text: Sample of MIMULUS GUTTATUS


Sample FBFC

characteristic: INSDC last update

text: live

text: Generic

text: 454 sequencing of PARVIBACULUM LAVAMENTIVORANS DS-1

text: Parvibaculum lavamentivorans DS-1

text: Sample of PARVIBACULUM LAVAMENTIVORANS DS-1


Sample FFFH

text: live

text: Generic

text: 454 sequencing of PETROTOGA MOBILIS SJ95

characteristic: description

text: Petrotoga mobilis SJ95

text: Sample of PETROTOGA MOBILIS SJ95


Sample FBHS

characteristic: INSDC secondary accession

text: live

text: Generic

text: 454 sequencing of PROCHLOROCOCCUS SP. NATL2A

text: Prochlorococcus marinus str. NATL2A

text: Sample of PROCHLOROCOCCUS SP. NATL2A


Sample FBFW

text: live

characteristic: INSDC status

text: Generic

text: 454 sequencing of PSEUDOMONAS PUTIDA F1

text: Pseudomonas putida F1

text: Sample of PSEUDOMONAS PUTIDA F1


Sample FBFG

text: live

text: Generic

characteristic: NCBI submission model

text: 454 sequencing of PSEUDOMONAS PUTIDA GB-1

text: Pseudomonas putida GB-1

text: Sample of PSEUDOMONAS PUTIDA GB-1


Sample FBHG

text: live

text: Generic

text: 454 sequencing of PYROBACULUM CALIDIFONTIS JCM 11548

text: Pyrobaculum calidifontis JCM 11548

text: Sample of PYROBACULUM CALIDIFONTIS JCM 11548


Sample FBGW

text: live

text: Generic

text: 454 sequencing of SHEWANELLA BALTICA OS195

text: Shewanella baltica OS195

text: Sample of SHEWANELLA BALTICA OS195


Sample FFFN

text: live

text: Generic

text: 454 sequencing of SHEWANELLA BALTICA OS223

text: Shewanella baltica OS223

text: Sample of SHEWANELLA BALTICA OS223


Sample FBGG

text: live

text: Generic

text: 454 sequencing of SHEWANELLA PUTREFACIENS CN-32

text: Shewanella putrefaciens CN-32

text: Sample of SHEWANELLA PUTREFACIENS CN-32


Sample FBGO

text: live

text: Generic

text: 454 sequencing of SHEWANELLA SP. PV-4

text: Shewanella loihica PV-4

text: Sample of SHEWANELLA SP. PV-4


Sample FFFI

text: live

text: Generic

text: 454 sequencing of STENOTROPHOMONAS MALTOPHILIA R551-3

text: Stenotrophomonas maltophilia R551-3

text: Sample of STENOTROPHOMONAS MALTOPHILIA R551-3


Sample FBGS

text: live

text: Generic

text: 454 sequencing of THERMOANAEROBACTER ETHANOLICUS X514

text: Thermoanaerobacter sp. X514

text: Sample of THERMOANAEROBACTER ETHANOLICUS X514


Sample FBHB

text: live

text: Generic

text: 454 sequencing of THERMOSINUS CARBOXYDIVORANS NOR1

text: Thermosinus carboxydivorans Nor1

text: Sample of THERMOSINUS CARBOXYDIVORANS NOR1


Sample FBGB

text: Joint Genome Institute, U.S. Department of Energy

text: live

text: Generic

text: 454 sequencing of THERMOSIPHO MELANESIENSIS BI429

text: Thermosipho melanesiensis BI429

text: Sample of THERMOSIPHO MELANESIENSIS BI429


characteristic: Concentration of acetate

characteristic: Concentration of ethanol

characteristic: Concentration of fructose

characteristic: Concentration of glucose

characteristic: Concentration of lactate

characteristic: Concentration of mannitol

characteristic: Concentration of sucrose

text: School of Biological Sciences, Chung-Ang University

text: live

text: Generic

characteristic: amount or size of sample collected

characteristic: collection date

text: Community DNA obtained by 454 GS FLX titanium sequencing from sample at 1days of kimchi fermentation

text: food production factory

characteristic: environment (biome)

text: biotic mesoscopic physical object

characteristic: environment (feature)

text: fermented food product

characteristic: environment (material)

text: miscellaneous natural or artificial environment

characteristic: environmental package

characteristic: fermentation temperature

text: An industrial-scale batch of kimchi was prepared by Daesang F and F Corporation using their production line. Brie?y, Chinese cabbages (Brassica rapa subsp. pekinensis) were soaked in a solution of 15% (wt/vol) solar salt for 5 to 6 h. The soaked Chinese cabbages were washed with tap water three times and drained of excessive water. These salted cabbages were then mixed with various seasonings, including red pepper powder, radish, garlic, ginger, green onion, sugar, and fermented seafood (jeotgal), and were packaged into airtight plastic bags in 1-kg portions. Thirty 1-kg kimchi bags were stored at the standard fermentation temperature (4°C).

characteristic: food preparation method

text: Repubilc of Korea

text: not given

text: Chinese cabbage (main), red pepper powder, radish, garlic, ginger, green onion, sugar, and fermented seafood (jeotgal),

characteristic: ingredients

text: FastDNA Spin kit (MPbio)

characteristic: nucleic acid extraction method

text: food metagenome

characteristic: period of fermentation

characteristic: ph

text: anaerobic

characteristic: relationship to oxygen

text: The kimchi contents were ?ltered through three layers of coarse gauze (Daehan, Republic of Korea) to remove only large kimchi particles, and the ?ltrates were pooled. The pH of each ?ltrate was measured immediately, and the ?ltrates were centrifuged (8,000 rpm for 20min at 4C) to harvest microorganisms. The pellets and supernatants were stored at 80°C for extraction of bulk genomic DNA and metabolite analysis, respectively.

characteristic: sample collection device or method

characteristic: sample material processing

text: Food metagenome ; Metagenomic study of an J1 community from a kimchi, korean traditional fermented food.

characteristic: volume of sample


characteristic: Concentration of acetate

characteristic: Concentration of ethanol

characteristic: Concentration of fructose

characteristic: Concentration of glucose

characteristic: Concentration of lactate

characteristic: Concentration of mannitol

characteristic: Concentration of sucrose

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

characteristic: INSDC secondary accession

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

characteristic: SRA accession

text: Community DNA obtained by 454 GS FLX titanium sequencing from sample at 7days of kimchi fermentation

characteristic: description

text: food production factory

text: biotic mesoscopic physical object

text: fermented food product

text: Repubilc of Korea

text: not given

text: FastDNA Spin kit (MPbio)

text: food metagenome

characteristic: organism

text: anaerobic

text: Food metagenome; Metagenomic study of an J7 community from a kimchi, korean traditional fermented food.

characteristic: title


characteristic: Concentration of acetate

characteristic: Concentration of ethanol

characteristic: Concentration of fructose

text: live

text: Generic

characteristic: amount or size of sample collected

characteristic: collection date

text: Community DNA obtained by 454 GS FLX titanium sequencing from sample at 13days of kimchi fermentation

text: food production factory

characteristic: environment (biome)

text: biotic mesoscopic physical object

characteristic: environment (feature)

text: fermented food product

characteristic: environment (material)

characteristic: environmental package

characteristic: fermentation temperature

characteristic: food preparation method

text: Repubilc of Korea

text: not given

characteristic: ingredients

text: FastDNA Spin kit (MPbio)

characteristic: nucleic acid extraction method

text: food metagenome

characteristic: period of fermentation

characteristic: ph

text: anaerobic

characteristic: relationship to oxygen

characteristic: sample collection device or method

characteristic: sample material processing

text: Food metagenome; Metagenomic study of an J13 community from a kimchi, korean traditional fermented food.

characteristic: volume of sample


characteristic: Concentration of acetate

characteristic: Concentration of ethanol

characteristic: Concentration of fructose

characteristic: Concentration of glucose

characteristic: Concentration of lactate

characteristic: Concentration of mannitol

characteristic: Concentration of sucrose

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

characteristic: INSDC secondary accession

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

characteristic: SRA accession

text: Community DNA obtained by 454 GS FLX titanium sequencing from sample at 16days of kimchi fermentation

characteristic: description

text: food production factory

text: biotic mesoscopic physical object

text: fermented food product

text: Repubilc of Korea

text: not given

text: FastDNA Spin kit (MPbio)

text: food metagenome

characteristic: organism

text: anaerobic

text: Food metagenome; Metagenomic study of an J16 community from a kimchi, korean traditional fermented food.

characteristic: title


characteristic: Concentration of acetate

characteristic: Concentration of ethanol

characteristic: Concentration of fructose

text: live

text: Generic

characteristic: amount or size of sample collected

characteristic: collection date

text: Community DNA obtained by 454 GS FLX titanium sequencing from sample at 18days of kimchi fermentation

text: food production factory

characteristic: environment (biome)

text: biotic mesoscopic physical object

characteristic: environment (feature)

text: fermented food product

characteristic: environment (material)

characteristic: environmental package

characteristic: fermentation temperature

characteristic: food preparation method

text: Repubilc of Korea

text: not given

characteristic: ingredients

text: FastDNA Spin kit (MPbio)

characteristic: nucleic acid extraction method

text: food metagenome

characteristic: period of fermentation

characteristic: ph

text: anaerobic

characteristic: relationship to oxygen

characteristic: sample collection device or method

characteristic: sample material processing

text: Food metagenome; Metagenomic study of an J18 community from a kimchi, korean traditional fermented food.

characteristic: volume of sample


characteristic: Concentration of acetate

characteristic: Concentration of ethanol

characteristic: Concentration of fructose

characteristic: Concentration of glucose

characteristic: Concentration of lactate

characteristic: Concentration of mannitol

characteristic: Concentration of sucrose

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

characteristic: INSDC secondary accession

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

characteristic: SRA accession

text: Community DNA obtained by 454 GS FLX titanium sequencing from sample at 21days of kimchi fermentation

characteristic: description

text: food production factory

text: biotic mesoscopic physical object

text: fermented food product

text: Repubilc of Korea

text: not given

text: FastDNA Spin kit (MPbio)

text: food metagenome

characteristic: organism

text: anaerobic

text: Food metagenome; Metagenomic study of an J21 community from a kimchi, korean traditional fermented food.

characteristic: title


characteristic: Concentration of acetate

characteristic: Concentration of ethanol

characteristic: Concentration of fructose

text: School of Biological Sciences, Chung-Ang University

text: live

text: Generic

characteristic: amount or size of sample collected

characteristic: collection date

text: Community DNA obtained by 454 GS FLX titanium sequencing from sample at 23days of kimchi fermentation

text: food production factory

characteristic: environment (biome)

text: biotic mesoscopic physical object

characteristic: environment (feature)

text: fermented food product

characteristic: environment (material)

characteristic: environmental package

characteristic: fermentation temperature

characteristic: food preparation method

text: Repubilc of Korea

text: not given

characteristic: ingredients

text: FastDNA Spin kit (MPbio)

characteristic: nucleic acid extraction method

text: food metagenome

characteristic: period of fermentation

characteristic: ph

text: anaerobic

characteristic: relationship to oxygen

characteristic: sample collection device or method

characteristic: sample material processing

text: Food metagenome; Metagenomic study of an J23 community from a kimchi, korean traditional fermented food.

characteristic: volume of sample