text: Texas A&M University

text: live

text: Generic

text: Appressorium

text: Unidirectional cloning. EcoRI side has T3 primer and predominantly 5' reads. T7 primer on XhoI side of insert. Appressorium library. Conidia were germ inated on an inductive surface for 5-8 h. Sequences were processed by one of two methods. Where a full-length alignment to the M. grisea genome sequence was available, the EST sequence was trimed according to the alignment, otherwise sequence quality was assessed using phredPhrap version 991019 and trimmed according to phd files (0.05) and for vector seqs.

text: Pyricularia grisea

text: EcoRI

text: XhoI

text: Mat1-2 hermaphrodite

text: Magnaporthe grisea Ap Uni-Zap XR Library

text: Phagemid

text: pBluescriptSK+


status: PUBLIC

characteristic: External Id

text: Affymetrix, Inc.

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Homo sapiens

characteristic: organism

text: Affymetrix mapping of human transcriptome

characteristic: title


text: Washington University School of Medicine

text: live

text: Generic

text: cDNA primed using oligo-dT primer, directionally cloned into UDG sites of pAMP1. Size selected for insert sizes ranging from 0.2-2.0 kb. Normalized to Cot10. Primary library, non-amplified. Library constructed M. Lovett. For more information on this library, please visit the COGEN website http://hg.wustl.edu/COGENE/.

characteristic: description

text: 8.5 week embryo

tag: attribute

characteristic: dev-stage

characteristic: lab-host

text: Homo sapiens

text: no restr sites used in cloning

text: no restr sites used in cloning

text: Tongue

characteristic: tissue-type

text: 8 5 week embryo anterior tongue 8 5 EAT

text: phagemid

characteristic: vector


text: Harvard University, Howard Hughes Medical Institute

text: live

text: Generic

text: Both wildtype (target) and ngn3-/- (driver) cDNA libraries were amplified. The ssDNA from the wildtype cDNA library was prepared by infecting with M13KO7 and blocked with 5'GCGGCCGCT(15) oligo before hybridization. SalI digested dsDNA from the ngn3-/- cDNA library was used to synthesize biotinylated RNA with T7 RNA polymerase. Subtractive hybridization was performed at 42oC in 80% formamide, 100 mM HEPES pH 7.5, 2mM EDTA and 0.2% SDS. Streptavidin was used to remove clones common between the two libraries. After repair of the subtracted ssDNA with 5'GCGGCCGCT(15) oligo using Taq polymerase and Vent polymerase, the subtracted cDNA library was digested with SpeI and electroporated into DH12S cells (LTI).

text: Mus musculus

text: Not I

text: Sal I

text: 129/Sv x CD1

characteristic: strain

text: pancreas

text: Kaestner ngn3 - - subtracted

text: Plasmid (Amp resistant)

text: pSPORT1 (GIBCO)


text: live

text: Generic

text: The SMARTtm PCR cDNA synthesis Kit (CLONETECH) was used to obtain full-length cDNA from E14.5 wildtype mRNA. A modified 3' oligo(dT) containing a NotI site was used to prime the first-strand synthesis reaction to allow directional cloning. This full-length cDNA was used for PCR amplification to generate ds cDNA. After size selection (>1kb), SalI adapters were ligated onto both ends of the control cDNA, digested with NotI and cloned into pSPORT1 vector (LTI). The library was electroporated into DH12S cells (LTI) and semi-solid amplified. E14.5 pancreatic primordium was a gift from Dr. Gradwohl.

text: Mus musculus

text: Not I

text: Sal I

text: 129/Sv x CD1

text: pancreas

text: Kaestner wt amplified

text: Plasmid (Amp resistant)

text: pSPORT1 (GIBCO)


text: USDA/ARS, Plant Genetic Resources

text: live

text: Generic

text: Jewel

characteristic: cultivar

text: Ipomoea batatas

text: Entire in vitro plantlet

text: Sweetpotato


text: USDA, ARS, Beltsville Agricultural Research Center

text: live

text: Generic

text: Non-normalized library made from pooled mRNA isolated from mammary tissues at eight physiological, developmental, and disease states.

text: Bos taurus

text: XbaI

text: XhoI

text: pooled

text: BARC BMG-NN

text: plasmid

text: pCMV SPORT6


text: live

text: Generic

text: The library was constructed by Claire Murphy, Brandi Chiapelli and Dr. James McCarter at Washington University, St. Louis. The cDNA was made by using Dynabead oligo-dT priming (Dynal). PCR based library using a modified protocol from the SMART PCR cDNA Synthesis Kit from Clontech. Directionally cloned into the UDG sites of pAMP1. Parasitic adult females were collected from naive animals and provided by Dr. Mark Viney of Bristol, UK.

text: parasitic adult females

text: Strongyloides ratti

text: NotI

text: SalI

text: Strongyloides ratti PA female naive pAMP1 v1

text: plasmid (ampicillin resistant)

text: pAMP1 (Gibco)


text: live

text: Generic

text: The library was constructed by Claire Murphy and Dr. James McCarter at Washington University, St. Louis. The cDNA was made by using Dynabead oligo-dT priming (Dynal). PCR based library using a modified protocol from the SMART PCR cDNA Synthesis Kit from Clontech. Directionally cloned into the UDG sites of pAMP1. Nematodes were provided by Dr. Louis Gasbarree of the USDA, Beltsville, MD (lgasbarr@anri.barc.usda.gov). Late fourth stage larvae were recovered 10 days after infection of young calves with Ostertagia L3. The calves were killed and the abomasa (gastric glands) were recovered and washed extensively and then placed in a Baermann apparatus containing warm PBS for approximately 4 hours. The L4 that migrated from the tissues were washed extensively, pelleted and snap frozen in liquid nitrogen.

text: Ostertagia ostertagi

text: NotI

text: SalI

text: Ostertagia ostertagi L4 pAMP1 v1

text: plasmid (ampicillin resistant)

text: pAMP1 (Gibco)


text: live

text: Generic

text: The library was constructed by Claire Murphy and Dr. James McCarter at Washington University, St. Louis. The cDNA was made by using Dynabead oligo-dT priming (Dynal). PCR based library using a modified protocol from the SMART PCR cDNA Synthesis Kit from Clontech. Directionally cloned into the UDG sites ofpAMP1.

text: parasitic adult females

text: Meloidogyne hapla

text: NotI

text: SalI

text: Meloidogyne hapla egg pAMP1 v1

text: plasmid (ampicillin resistant)

text: pAMP1 (Gibco)


text: Oregon Health Sciences University

text: live

text: Generic

text: trabecular meshwork

characteristic: cell type

text: Human cdNA library made from mRNA isolated from trabecular meshwork cells established fom eyes from 6 individuals, ages 2 weeks to 2 years. Cells were harvested at passages 3 through 6. Invitrogen made a unidirectional cDNA library from the mRNA from the frozen cells using a pcDNA3 vector and TPO10F'' host cells.

text: 2 week to 2 year old infants

text: Homo sapiens

text: EcoRI

text: EcoRI

text: eye

text: Human Trabecular Meshwork cDNA library

text: plasmid


characteristic: External Id

text: live

text: Generic

text: The library was constructed by Amy Hofer, Claire Murphy and Dr. James McCarter at Washington University, St. Louis. The cDNA was made by using Dynabead oligo-dT priming (Dynal). PCR based library using a modified protocol from the SMART PCR cDNA Synthesis Kit from Clontech. Directionally cloned into the UDG sites of pAMP1. Irradiated and cultured nematodes were provided by Dr. Ben-Wen Li and Dr. Gary Weil of Washington University, St. Louis, MO (weillab@imgate.wustl.edu).

text: irradiated 2 day cultured L3

text: Brugia malayi

text: NotI

text: SalI

text: Brugia malayi 2D L3 irradiated pAMP1 v1

text: plasmid (ampicillin resistant)

text: pAMP1 (Gibco)


text: Tianjin Medical University

characteristic: INSDC center name

text: live

text: Generic

text: Homo sapiens

text: gastric cancer

text: Homo sapiens gastric cancer


text: Tianjin Medical University

characteristic: INSDC first public

text: live

text: Generic

text: Homo sapiens

text: gastric mucosa

text: Homo sapiens gastric mucosa


text: Institute of Basic Medical Sciences, CAMS&PUMC

characteristic: INSDC last update

text: live

text: Generic

text: A substractive cDNA library was constructed (CLONTECH PCR-Select cDNA Subtraction Kit), using kidney mRNA of h-apoE transgenic mice to synthetize the tester cDNA, and cDNA obtained from kidney mRNA of normal mice as driver. Differential expression was confirmed by hybridization with cDNA probes. The method used yielded RsaI digested cDNA fragments of sizes ranging from 150 to 800 bp, which were fully sequenced.

text: adult

text: Mus musculus

text: EcoR I

text: EcoR I

text: male

characteristic: sex

text: Kidney subtractive library

text: pGEM-T


text: National Institute of Mental Health

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: NIH_BMAP_DJ0 is a cDNA library containing the following tissues: subfornical organ and postrema. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is GCTACATGAT.

text: Adult

text: DH10B (Life Technologies) (T1 phage resistant)

text: brain

characteristic: organ

text: Mus musculus

text: EcoR I

text: Not I

text: subfornical organ and postrema

text: Plasmid

text: pT7T3-Pac (Pharmacia) with a modified polylinker


text: National Institute of Mental Health

text: live

text: Generic

characteristic: NCBI submission package

text: NIH_BMAP_DJ1 is a cDNA library containing the following tissues: subfornical organ and postrema. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is GCTACATGAT.

text: Adult

text: brain

text: Mus musculus

characteristic: organism

text: EcoR I

text: Not I

text: subfornical organ and postrema

characteristic: title

text: Plasmid


text: University of Illinois

text: live

text: Generic

text: A subtracted cDNA library was made from corneas undergoing transdifferentiation to form lenses.

characteristic: description

characteristic: dev-stage

characteristic: lab-host

text: Xenopus laevis

text: NotI

text: SalI

text: Cornea

text: Cornea-lens transdifferentiation library

text: Plasmid


text: Univ Res Ctr for Neuroendocrinology, University of Bristol

text: live

text: Generic

text: This cDNA library is a subtracted library derived from the rat hypothalamic Supra-Optic Nucleus and generated with SSH. For a detailed description contact the submitter.

text: adult

text: Brain

text: Rattus norvegicus

text: Male

text: Sprague-Dawley

characteristic: strain

text: Hypothalamic supra-optic nucleus

characteristic: tissue-type

text: Rat hypothalamic SON cDNA library generated with SSH

text: pT-Adv (Clontech)

characteristic: vector


characteristic: External Id

text: Institute of Plant Science

text: live

text: Generic

text: three leaves

text: E.coli (TOP 10F)

text: Oryza sativa

text: root

text: rice root nitrate-induced subtracted library

text: pT-Adv


text: live

text: Generic

text: The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. Denatured mRNa was size fractionated on a 1% agarose gel. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was size selected according to mRNA size fraction, ligated with EcoR I adaptor, digested with Not I, and then cloned directionally into pYX-Asc vector. The library tag sequence located between the Not I site and the polyA tail, is CAGCCACGAC. This library was created for the University of Iowa Mouse Brain Molecular Anatomy Project (BMAP): 'Gene Discovery in the Developing Mouse Nervous System', supported by National Institute of Mental Health (NIMH), Hemin Chin, Ph.D., program coordinator.

text: embryo 18.5 dpc

text: DH10B (T1 phage resistant)

text: brain

text: Mus musculus

text: EcoR I

text: Not I

text: whole brain

text: Plasmid

text: pYX-Asc


text: University of California at San Francisco

characteristic: INSDC center name

text: live

text: Generic

text: high endothelial

characteristic: cell type

text: High endothelial cells (HEC) were isolated from human tonsils using magnetic beads derivatized with MECA-79 antibody specific to the peripheral lymph node addressin. cDNA was prepared from these cells using Cap-Finder methodology, then cDNA prepared from tonsillar lymphocytes and from human umbilical vein endothelial cells were subtracted from the HEC cDNA, followed by normalization through selective PCR amplification.

text: tonsil

text: Homo sapiens

text: EcoRI

text: EcoRI

text: HEV PCR-select

text: Plasmid

text: pCRII


text: Graduate School of Bioagricultural Sciences, Nagoya University

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Haruna-nijyo

characteristic: cultivar

text: early vegetative stage

text: Hordeum vulgare subsp. vulgare

text: root

text: salt-stressed barley root cDNA


text: Instituto de Desarrollo Regional

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: C4 (dormancy)

text: Corm

text: Crocus sativus

text: Saffron Corm C4 Lambda Zap Express Library


text: University of Tsukuba

text: live

text: Generic

characteristic: NCBI submission package

text: Ciona intestinalis

text: testis

text: K. Inaba unpublished cDNA library, testis


text: Tokyo Institiute of Technology

text: live

text: Generic

text: 110-cell embryo

text: Halocynthia roretzi

characteristic: organism

text: cDNA library of 110-cell embryo mRNAs subtracted with fertilized egg mRNAs


text: University of Helsinki

text: live

text: Generic

text: Saana

text: 1 day after pollination

text: Embryo

text: Hordeum vulgare subsp. vulgare

characteristic: title


text: University of Helsinki

text: live

text: Generic

text: 12,15,18 days after pollination

characteristic: description

text: Developing seed

text: Hordeum vulgare


text: University of Helsinki

text: live

text: Generic

text: 2-,3-,4-days after germination

text: Shoot

characteristic: dev-stage

text: Hordeum vulgare


text: Institut National Polytechnique de Toulouse - Ecole National Superieure Agronomique de Toulouse

text: live

text: Generic

text: protoplasts

text: Emil

text: 1-5 days

text: Helianthus annuus

text: hypocotyls


text: Crebs

text: live

text: Generic

text: one day after inoculation with Phytophthora infestans

text: Solanum phureja x Solanum tuberosum

text: leaf

text: Solanum phureja x Solanum tuberosum leaf


text: live

text: Generic

text: The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. Denatured mRNa was size fractionated on a 1% agarose gel. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was size selected according to mRNA size fraction, ligated with EcoR I adaptor, digested with Not I, and then cloned directionally into pYX-Asc vector. The library tag sequence located between the Not I site and the polyA tail, is CAGCCACGAC. This library was created for the University of Iowa Mouse Brain Molecular Anatomy Project (BMAP): 'Gene Discovery in the Developing Mouse Nervous System', supported by National Instututes of Mental Health (NIMH), Hemin Chin, Ph.D., program coordinator.

text: embryo 15.5 dpc

text: DH10B (T1 phage resistant)

characteristic: lab-host

text: brain

characteristic: organ

text: Mus musculus

text: EcoR I

text: Not I

text: whole brain

text: Plasmid

text: pYX-Asc


text: Washington University School of Medicine

text: live

text: Generic

text: The library was donated by Dr. T. Geary, Pharmacia Inc, Kalamazoo, MI,(tggeary@am.pnu.com). The library was created from dissected whole female gonads. DNA sequencing was done by Washington University Genome Sequencing Center, St. Louis, MO.

text: Adult

text: Ascaris suum

text: EcoRI

text: EcoRI

text: Female

characteristic: sex

text: Ovary

characteristic: tissue-type

text: Ascaris suum female ovary

text: Lambda Zap II(Stratagene)


characteristic: External Id

text: live

text: Generic

text: The library was constructed by Claire Murphy and Dr. James McCarter at Washington University, St. Louis. The cDNA was made by using Dynabead oligo-dT priming (Dynal). PCR based library using a modified protocol from the SMART PCR cDNA Synthesis Kit from Clontech. Directionally cloned into the UDG sites of pAMP1. Adult nematodes were collected from infected dogs and provided by Dr. Prema Arasu of North Carolina State University, Raleigh,NC (Prema_Arasu@ncsu.edu).

text: adult

text: Toxocara canis

text: NotI

text: SalI

text: Toxocara canis adult pAMP1 v1

text: plasmid (ampicillin resistant)

text: pAMP1 (Gibco)

characteristic: vector


text: University of Iowa

characteristic: INSDC center name

text: live

text: Generic

text: UI-CF-EN1 is a normalized cDNA library containing the following tissue(s): Primary Lung Cystic Fibrosis Epithelial Cells. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CTGCTCAGGT.

text: Adult

text: DH10B (Life Technologies) (T1 phage resistant)

text: Lung

text: Homo sapiens

text: EcoR I

text: Not I

text: Plasmid

text: pT7T3-Pac (Pharmacia) with a modified polylinker


text: University of Iowa

characteristic: INSDC first public

text: live

text: Generic

text: UI-CF-EN0 is a cDNA library containing the following tissue(s): Primary Lung Cystic Fibrosis Epithelial Cells. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CTGCTCAGGT.

text: Adult

text: Lung

text: Homo sapiens

text: EcoR I

text: Not I

text: Plasmid


text: University of Iowa

characteristic: INSDC last update

text: live

text: Generic

text: UI-CF-EC1 is a normalized cDNA library containing the following tissue(s): Normal lung from adult and from fetal day 64, day 87, week 19 and week 42. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is AAGTGCTTAC.

text: Adult and Fetal

text: Lung

text: Homo sapiens

text: EcoR I

text: Not I

text: Lung

text: Plasmid


text: University of Iowa

text: live

text: Generic

text: UI-CF-EC0 is a cDNA library containing the following tissue(s): Normal lung from adult and from fetal day 64, day 87, week 19 and week 42. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is AAGTGCTTAC.

text: Adult and Fetal

text: Lung

text: Homo sapiens

text: EcoR I

text: Not I

text: Lung

text: Plasmid


text: University of Iowa

text: live

text: Generic

text: UI-CF-DU0 is a cDNA library containing the following tissue(s): Primary Lung Epithelial Cells The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is GGCTGTAGGC.

text: Adult

text: Lung

text: Homo sapiens

text: EcoR I

text: Not I

text: Primary Lung Epithelial Cells

text: Plasmid


text: University of Iowa

text: live

text: Generic

text: UI-CF-DU1 is a normalized cDNA library containing the following tissue(s): Primary Lung Epithelial Cells The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is GGCTGTAGGC.

text: Adult

text: Lung

text: Homo sapiens

text: EcoR I

text: Not I

text: Primary Lung Epithelial Cells

text: Plasmid


text: European Molecular Biology Laboratory

text: live

text: Generic

text: ESTs sequenced from the T7 priming site that reads from the 5' end of cDNA. The NAP1 is a directionally cloned and normalized, oligo-T primed cDNA library constructed from heads of Anopheles gambiae according to: Bonaldo, Lennon & Soares (1996): Normalization and Subtraction: Two Approaches To Facilitate Gene Discovery, Genome Research 6, 791-806.

text: E. coli DH10B

text: Anopheles gambiae

text: NotI

text: EcoRI

text: NAH


text: University of Notre Dame

text: live

text: Generic

text: ESTs sequenced from the T7 priming site that reads from the 5' end of cDNA. The NAP1 is a directionally cloned and normalized, oligo-T primed cDNA library constructed from a mixture of Anopheles gambiae developmental stages according to: Bonaldo, Lennon & Soares (1996): Normalization and Subtraction: Two Approaches To Facilitate Gene Discovery, Genome Research 6, 791-806.

text: E. coli DH10B

text: Anopheles gambiae

text: NotI

text: EcoRI


text: University of Illinois

text: live

text: Generic

text: Eutrema halophilum

text: Ath

text: pBK-CMV phagemid


text: live

text: Generic

text: NCI_CGAP_Pl8 is a cDNA library containing the following tissue(s): Placenta 20 dpc. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is GTGTG.

text: 20 dpc

text: DH10B (Life Technologies)

text: Placenta

text: Mus musculus

text: EcoR I

text: Not I

characteristic: strain

text: Placenta

text: Plasmid


text: live

characteristic: INSDC status

text: Generic

text: NCI_CGAP_Pl1 is a cDNA library containing the following tissue(s): placenta 8-9 weeks pregnant. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is GA.

text: 8-9 weeks

text: DH10B (Life Technologies)

text: Placenta

text: Homo sapiens

text: EcoR I

text: Not I

text: Placenta

text: Plasmid


text: live

text: Generic

characteristic: NCBI submission model

text: NCI_CGAP_Pl2 is a subtracted cDNA library constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tags for this library are GA, AGGAA. For additional information, contact: Bento Soares, bento-soares@uiowa.edu

text: 8-9 weeks

text: DH10B (Life Technologies)

text: Placenta

text: Homo sapiens

text: EcoR I

text: Not I

text: Placenta

text: Plasmid


text: live

text: Generic

characteristic: NCBI submission package

text: NCI_CGAP_Pl3 is a subtracted cDNA library constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tags for this library are GA, AGGAA. For additional information, contact: Bento Soares, bento-soares@uiowa.edu

text: 8-9 weeks

text: DH10B (Life Technologies)

text: Placenta

text: Homo sapiens

text: EcoR I

text: Not I

text: Placenta

text: Plasmid


text: live

text: Generic

text: NCI_CGAP_PlTr1 is a subtracted cDNA library constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tags for this library are GTGTG, ATCAT, GGGTG, GGTTG. For additional information, contact: Bento Soares, bento-soares@uiowa.edu

text: DH10B (Life Technologies)

text: Placenta

text: Mus musculus

characteristic: organism

text: EcoR I

text: Not I

text: Trophoblast

text: Plasmid


text: live

text: Generic

text: NCI_CGAP_Pl7 is a cDNA library containing the following tissue(s): Placenta Choriocarcinomai JAR Line (ATCC). The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is AGGCATCACA.

text: DH10B (Life Technologies)

text: Placenta

text: Homo sapiens

text: EcoR I

text: Not I

text: Placenta Choriocarcinoma

characteristic: title

text: Plasmid


text: National Cancer Institute / NIH

text: live

text: Generic

text: NCI_CGAP_DF1 is a normalized cDNA library containing the following tissue(s): Subchondral Bone. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is GTTAAGCGTC.

characteristic: description

text: Adult

text: DH10B (Life Technologies)

text: Bone

text: Homo sapiens

text: EcoR I

text: Not I

text: Subchondral Bone

text: Plasmid


text: live

text: Generic

text: NCI_CGAP_DF0 is a cDNA library containing the following tissue(s): Subchondral Bone. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is GTTAAGCGTC.

text: Adult

characteristic: dev-stage

text: DH10B (Life Technologies)

text: Bone

text: Homo sapiens

text: EcoR I

text: Not I

text: Subchondral Bone

text: Plasmid


text: live

text: Generic

text: NCI_CGAP_DT0 is a cDNA library containing the following tissue(s): Metatastic Chondrosarcoma in Lung. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is AACTGTTCGG.

text: Adult

text: DH10B (Life Technologies)

characteristic: lab-host

text: Lung

text: Homo sapiens

text: EcoR I

text: Not I

text: Metastatic Chondrosarcoma

text: Plasmid


text: National Cancer Institute / NIH

text: live

text: Generic

text: NCI_CGAP_Car1 is a cDNA library containing the following tissue(s): Osteoarthritic Cartilage The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is TGATCACGCT.

text: Adult

text: DH10B (Life Technologies)

text: Knee

characteristic: organ

text: Homo sapiens

text: EcoR I

text: Not I

text: Osteoarthritic Cartilage

text: Plasmid

text: pT7T3-Pac (Pharmacia) with a modified polylinker


text: live

text: Generic

text: NCI_CGAP_DT1 is a normalized cDNA library containing the following tissue(s): Metatastic Chondrosarcoma in Lung. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is AACTGTTCGG.

text: Adult

text: DH10B (Life Technologies)

text: Lung

text: Homo sapiens

text: EcoR I

text: Not I

text: Metastatic Chondrosarcoma

text: Plasmid


text: live

text: Generic

text: NCI_CGAP_Fs1 is a cDNA library containing the following tissue(s): Fibrosarcoma Cell line HT-1088 (ATCC number CCL-121). The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is GTTCTACGAG.

text: DH10B (Life Technologies)

text: Homo sapiens

text: EcoR I

text: Not I

text: Fibrosarcoma

text: Plasmid


text: National Cancer Institute / NIH

text: live

text: Generic

text: NCI_CGAP_DI0 is a cDNA library containing the following tissue(s): A pool of Lung Focal Fibrosis. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is ATACGCGGTC.

text: Adult

text: DH10B (Life Technologies)

text: Lung

text: Homo sapiens

text: EcoR I

text: Not I

text: Lung Focal Fibrosis

text: Plasmid


text: live

text: Generic

text: NCI_CGAP_DH1 is a normalized cDNA library containing the following tissue(s): VS-8 Cell line from Metastatic Chondrosarcoma in Lung. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is AGATCATTGC.

text: Adult

text: DH10B (Life Technologies)

text: Lung

text: Homo sapiens

text: EcoR I

text: Not I

text: Metastatic Chondrosarcoma

text: Plasmid


text: live

text: Generic

text: NCI_CGAP_DH0 is a cDNA library containing the following tissue(s): VS-8 Cell line from Metastatic Chondrosarcoma in Lung. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is AGATCATTGC.

text: Adult

text: DH10B (Life Technologies)

text: Lung

text: Homo sapiens

text: EcoR I

text: Not I

text: Metastatic Chondrosarcoma

text: Plasmid


text: live

text: Generic

text: NCI_CGAP_Pl9 is a cDNA library containing the following tissue(s): Placenta 11.5-12.5 dpc. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is ATCAT.

text: 11.5-12.5 dpc

text: DH10B (Life Technologies)

text: Placenta

text: Mus musculus

text: EcoR I

text: Not I

text: Placenta

text: Plasmid


text: live

text: Generic

text: NCI_CGAP_Pl5 is a subtracted cDNA library constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tags for this library are GA, AGGAA. For additional information, contact: Bento Soares, bento-soares@uiowa.edu

text: Full Term

text: DH10B (Life Technologies)

text: Placenta

text: Homo sapiens

text: EcoR I

text: Not I

text: Placenta

text: Plasmid


text: live

text: Generic

text: NCI_CGAP_Pl6 is a subtracted cDNA library constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tags for this library are GA, AGGAA. For additional information, contact: Bento Soares, bento-soares@uiowa.edu

text: Full Term

text: DH10B (Life Technologies)

text: Placenta

text: Homo sapiens

text: EcoR I

text: Not I

text: Placenta

text: Plasmid


text: live

text: Generic

text: NCI_CGAP_Pl4 is a cDNA library containing the following tissue(s): Placenta full term. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is AGGAA.

text: Full Term

text: DH10B (Life Technologies)

text: Placenta

text: Homo sapiens

text: EcoR I

text: Not I

text: Placenta

text: Plasmid


text: live

text: Generic

text: tissues: Cholonic mucosa with Crohns disease, Cholonic mucosa with ulcerative colitis, Fetal thymus, Cervix, Cervical adenosquamous carcinoma, Ligament cells, Prostate carcinoma, Bladder carcinoma, Brain oligodenroga ; NCI_CGAP_Sub9 is a subtracted cDNA library constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tags for this library are CGTC, AACG, GGGCC, GGAAG, TAGC, TAAGC, ATGG, AGACA, ATCAC. For additional information, contact: Bento Soares, bento-soares@uiowa.edu

text: mixed

text: DH10B (Life Technologies)

text: Homo sapiens

text: EcoR I

text: Not I

text: mixed

text: Plasmid


text: RIKEN Genomic Sciences Center

text: live

text: Generic

text: Subtraction Library. The sequence was obtained from samples subjected to cold-treated (1, 2, 5, 10, and 24 hr)

text: rosette

text: Arabidopsis thaliana

text: BamHI

text: SalI


text: RIKEN Genomic Sciences Center

text: live

text: Generic

text: Subtraction Library. The sequence was obtained from samples subjected to dehydration-treated (1, 2, 5, 10, and 24 hr)

text: rosette

text: Arabidopsis thaliana

text: BamHI

text: SalI


text: RIKEN Genomic Sciences Center

text: live

text: Generic

text: Arabidopsis thaliana

text: BamHI

text: SalI

text: root


text: RIKEN Genomic Sciences Center

text: live

text: Generic

text: Arabidopsis thaliana

text: BamHI

text: SalI

text: mixture of silique and flower


text: RIKEN Genomic Sciences Center

text: live

text: Generic

text: dark-grown

text: Arabidopsis thaliana

text: BamHI

text: SalI


text: RIKEN Genomic Sciences Center

text: live

text: Generic

text: Subtraction Library. The sequence was obtained from samples subjected to dehydration-treated (1, 2, 5, 10 and 24 hr) and rehydration-treated (1, 2, 5, 10, and 24 hr after dehydration treatment)

text: Arabidopsis thaliana

text: BamHI

text: SalI


text: RIKEN Genomic Sciences Center

text: live

text: Generic

text: Arabidopsis thaliana

text: BamHI

text: SalI


text: RIKEN Genomic Sciences Center

text: live

text: Generic

text: Subtraction Library

text: Arabidopsis thaliana

text: BamHI

text: SalI

text: mixture of silique and flower


text: RIKEN Genomic Sciences Center

text: live

text: Generic

text: Subtraction Library. The sequence was obtained from samples subjected to various stress and plant hormones-treated

text: Arabidopsis thaliana

text: BamHI

text: SalI


text: RIKEN Genomic Sciences Center

text: live

text: Generic

text: rosette plants

text: SOLR

text: Arabidopsis thaliana

text: SstI

text: XhoI


text: RIKEN Genomic Sciences Center

text: live

text: Generic

text: subjected to dehydration-treated(2hr)

text: rosette plants

text: SOLR

text: Arabidopsis thaliana

text: SstI

text: XhoI


text: RIKEN Genomic Sciences Center

text: live

text: Generic

text: subjected to cold-treated(1,2,5,10,24 hr)

text: rosette plants

text: SOLR

text: Arabidopsis thaliana

text: SstI

text: XhoI


text: RIKEN Genomic Sciences Center

text: live

text: Generic

text: subjected to dehydration-treated(1,2,5,10,24 hr)

text: rosette plants

text: SOLR

text: Arabidopsis thaliana

text: SstI

text: XhoI


text: RIKEN Genomic Sciences Center

text: live

text: Generic

text: subjected to dehydration (1, 2, 5, 10, 24 hr) and cold (1, 2, 5, 10, 24 hr) treatments

text: plants at various developmental stages from germination to mature seeds

text: Arabidopsis thaliana

text: SstI

text: XhoI


text: RIKEN Genomic Sciences Center

text: live

text: Generic

text: subjected to cold-treated (1, 2, 5, 10, 24 hr)

text: rosette plants

text: Arabidopsis thaliana

text: BamHI

text: SalI


text: RIKEN Genomic Sciences Center

text: live

text: Generic

text: subjected to dehydration-treated (1, 2, 5, 10, 24 hr)

text: rosette plants

text: Arabidopsis thaliana

text: BamHI

text: SalI


text: RIKEN Genomic Sciences Center

text: live

text: Generic

text: Arabidopsis thaliana

text: BamHI

text: SalI


text: Washington University School of Medicine

text: live

text: Generic

text: Library constructed by Stavros Bashiardes and re-arrayed by A. Cuming & Honglin Rong. Construction of the cDNA library was carried out using Statagene's 'UniZAP - cDNA synthesis kit' to ligate cDNA directionally in UniZAP XR vector arms. The vector is designed containing the pBluescript sequence as well as the lambda DNA and cDNA is cloned in the EcoRI and XhoI sites in the pBluescript sequence. The vector was then packaged using Gold gigapackaging extracts, propagated in XL-IBlue MRF cels and amplified. The library was excied by mass excision using Stratagene's Mass excision kit to infect SOLR cells with phagemids and ampicillin resistant transformants selected. Approximately 1,000,000 colonies were grown and recovered by using Quiagen midi prep kit.2 micro grams of plasmid DNA were used to transform DH10b cells by electroporation. Clones corresponding to abundant transcripts were identified by colony hybridization and eliminated from the library, be rearraying. This library is non-directionally cloned.

text: protonemata, 7day old untreated

text: E.coli DH10b

text: Physcomitrium patens

text: XhoI

text: EcoRI

text: Moss EST library PPS

text: plasmid

text: pBluescript SK-


text: Peking University

text: live

text: Generic

text: RCC ssh Library

text: kidney

text: Homo sapiens

text: male

characteristic: sex

text: renal carcinoma

text: renal cell Express Library

text: pT-Adv


text: University College Dublin

text: live

text: Generic

text: primary mesangial cells

characteristic: cell type

text: Homo sapiens

text: Homo sapiens primary mesangial cells


characteristic: External Id

text: NRC Institute for Marine Biosciences

text: live

text: Generic

text: Libraries (SK2, SK3, SK4 and SK5) made by suppression subtractive hybridization using head kidney from salmon infected with Aeromonas salmonicida vs. uninfected salmon

text: adult

text: kidney

text: Salmo salar

text: Atlantic Salmon head kidney SSH

text: pT-Adv


text: NRC Institute for Marine Biosciences

text: live

text: Generic

text: Libraries (SL2, SL3, SL4 and SL5) made by suppression subtractive hybridization using liver from salmon infected with Aeromonas salmonicida vs. uninfected salmon

text: adult

text: liver

text: Salmo salar

text: Atlantic Salmon liver SSH

text: pT-Adv

characteristic: vector


text: NRC Institute for Marine Biosciences

characteristic: INSDC center name

text: live

text: Generic

text: Libraries (SS2, SS3, SS4 and SS5) made by suppression subtractive hybridization using spleen from salmon infected with Aeromonas salmonicida vs. uninfected salmon

text: adult

text: spleen

text: Salmo salar

text: Atlantic Salmon spleen SSH

text: pT-Adv


text: University of Delaware

characteristic: INSDC first public

text: live

text: Generic

text: E.coli EMDH10B

text: Gallus gallus

text: Male and Female

text: thymus, bursa, spleen, PBL, bone marrow

characteristic: tissue-type

text: normalized chicken lymphoid cDNA library

text: pCMVSPORT 6


text: University of Western Australia

characteristic: INSDC last update

text: live

text: Generic

text: Ovis aries

text: male

text: hypothalamus

text: Sheep partial cDNA


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

characteristic: INSDC status

text: Generic

text: cDNA made by oligo-dT priming. Directionally cloned into EcoRI/XhoI sites using the following 5' adaptor: GGCACGAG(G). Library constructed by Ling Hong in the laboratory of Gerald M. Rubin (University of California, Berkeley) using ZAP-cDNA synthesis kit (Stratagene) and Superscript II RT (Life Technologies). Note: this is a NIH_MGC Library.

text: DH10B (phage-resistant)

text: lung

text: Homo sapiens

text: EcoRI

text: XhoI

text: epidermoid carcinoma, cell line

text: plasmid (chloramphenicol resistant, grown at 30C)


text: The Institute for Genomic Research (TIGR; www.tigr.org)

text: live

text: Generic

characteristic: NCBI submission model

text: Kennebec

characteristic: cultivar

text: supplier: UC Berkeley, PGEC; sequencing: The Institute for Genomic Research. Whole plants were challenged with 450,000 sporangia/ml P. infestans isolate US-1 (US940501) in Biotron (Madison, Wisconsin). Leaf tissue was collected at 1, 2, 5, 12, and 24 hours post-challenge and frozen in liquid nitrogen immediately upon removal. Kennebec plants showed no signs of HR. Katahdin plants (susceptible to P. infestans US-1) were used as controls and showed infection. NOTE: We cannot exclude the possibility that this sequence is actually derived from Phytophthora rather than potato.

text: 6 week old

text: SOLR

text: Solanum tuberosum

text: EcoRI

text: XhoI

text: leaf

text: P. infestans-challenged potato leaf, incompatible reaction

text: pBluescript SK(-)


text: RIKEN Genomic Sciences Center

text: live

text: Generic

characteristic: NCBI submission package

text: subjected to various treatments (dehydration, cold, high salt, ABA, heat and UV). Dark-grown plants

text: Arabidopsis thaliana

characteristic: organism

text: BamHI

text: SalI


text: College of Life Science, Beijing Normal University

text: live

text: Generic

text: Oryza sativa

text: caryopsis

text: Unmature rice caryopsis cDNA

characteristic: title


text: live

text: Generic

text: Zea mays

text: seed

text: Unmature maize seed cDNA


text: Ludwig Institute for Cancer Research

text: live

text: Generic

text: obtained from differential display

characteristic: description

text: Homo sapiens

text: thyroid papillary carcinoma

text: Homo sapiens thyroid papillary carcinoma


text: Ludwig Institute for Cancer Research

text: live

text: Generic

text: obtained from differential display

text: Homo sapiens

text: thyroid adenoma

text: Homo sapiens thyroid adenoma


text: Henna Normal Univ.

text: live

text: Generic

text: Rattus norvegicus

text: male

text: Sprague-Dawley

characteristic: strain

text: liver

text: Rat Lambda Zap Express Library


text: Beijing Institute of Transfusion Medicine

text: live

text: Generic

text: fetal

characteristic: dev-stage

text: Mus musculus

text: whole

text: Mus musculus fetal cDNA library

text: pGEM-T-easy


text: Institute of Basic Medical Science

text: live

text: Generic

text: lung giant-cell-cancer cell

characteristic: cell-line

text: The library, which includes about 570 clones, was made from a pair of cell strains (PLA-801C and D) by SSH technology. Both strains were sub-clones of a cell line (PLA-801) derived from human lung-giant-cell cancer. D has the higher ability of metastasis then C. After implanting into the subcutis of nude mouse, D is capable of spontaneously metastasizing to lung and lymph nodes, whereas C is not. We used D as tester and C as driver in SSH. So the ESTs maybe parts of genes which have the ability of metastasis-promotion.

text: lung

characteristic: organ

text: Homo sapiens

text: male

characteristic: sex

text: subtracted library of lung cancer cell

text: Plasmid

text: pGEM-T easy


characteristic: External Id

text: Tokyo University of Agriculture and Technology

text: live

text: Generic

text: Bruguiera sexangula

text: Bruguiera sexangula mRNA


text: National Institute of Agrobiological Sciences

characteristic: INSDC center name

text: live

text: Generic

text: early embryo

text: Bombyx mori

text: embryo

text: Bombyx mori early embryo


text: Hokkaido University

characteristic: INSDC first public

text: live

text: Generic

text: suspension cultured cell

characteristic: cell type

text: Harosoy

text: differential display fragment from cells treated with syringolide

text: Glycine max

text: Glycine max Harosoy suspension cells


text: National Institute of Genetics

characteristic: INSDC last update

text: live

text: Generic

text: Chinese Spring

text: Feekes' scale 9

text: Triticum aestivum

text: spike at meiosis

characteristic: tissue-type

text: Y. Ogihara unpublished cDNA library, Wh


text: National Institute of Genetics

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Chinese Spring

text: Feekes' scale 1

text: Triticum aestivum

text: crown of seedling

text: Y. Ogihara unpublished cDNA library, Wh_dL


text: National Institute of Genetics

text: live

text: Generic

characteristic: NCBI submission package

text: Chinese Spring

characteristic: cultivar

text: Feekes' scale 11.2

text: Triticum aestivum

text: seed DPA10

text: Y. Ogihara unpublished cDNA library, Wh_e


text: National Institute of Genetics

text: live

text: Generic

text: Chinese Spring

text: Feekes' scale 10.5.1

text: Triticum aestivum

characteristic: organism

text: spike at flowering date

text: Y. Ogihara unpublished cDNA library, Wh_f

characteristic: title


text: Medical University of South Carolina

text: live

text: Generic

text: Penaeus vannamei

text: Sfi I

text: Sfi I

text: gill

text: LvG

text: Plasmid

characteristic: vector


text: live

text: Generic

text: fetal

characteristic: dev-stage

text: Homo sapiens

text: skeletal muscle

text: Human fetal skeletal muscle subtracted cDNA library

text: pT-Adv


text: National Institute of Genetics

text: live

text: Generic

text: Chinese Spring

text: Feekes' scale 10.5

text: Triticum aestivum

text: spike at heading date

text: Y. Ogihara unpublished cDNA library, Wh_h


text: National Institute of Genetics

text: live

text: Generic

text: Chinese Spring

text: Feekes' scale 10.5

text: Triticum aestivum

text: pistil at heading date

text: Y. Ogihara unpublished cDNA library, Wh_oh


text: National Institute of Genetics

text: live

text: Generic

text: Chinese Spring

text: Feekes' scale 1

text: Triticum aestivum

text: root

text: Y. Ogihara unpublished cDNA library, Wh_r


text: University of Manitoba

text: live

text: Generic

text: Homo sapiens

text: Homo sapiens clone


text: School of Life Sciences, Zhongshan University

text: live

text: Generic

text: leucocytes

text: Epinephelus coioides

text: SfiI

text: SfiI

text: female

text: orange-spotted grouper leukocytes lambda TriplEx2 Library

text: Phage

text: lambda TriplEx2


text: National Institute of Genetics

text: live

text: Generic

text: Chinese Spring

text: Feekes' scale 11.3

text: Triticum aestivum

text: seed DPA30

text: Y. Ogihara unpublished cDNA library, Wh_SL


text: National Institute of Genetics

text: live

text: Generic

text: Chinese Spring

text: Feekes' scale 6

text: Triticum aestivum

text: spikelet at late flowering

text: Y. Ogihara unpublished cDNA library, Wh_yd


text: National Institute of Genetics

text: live

text: Generic

text: Chinese Spring

text: Feekes' scale 6

text: Triticum aestivum

text: spikelet at early flowering

text: Y. Ogihara unpublished cDNA library, Wh_yf


text: Washington University School of Medicine

text: live

text: Generic

text: ***WARNING: Subsequent examination of these samples has revealed the presence of an additional Trichostrongyloidea cattle nematode, Cooperia oncophora. Sequences in this library may derive from either Ostertagia or Cooperia.*** The library was constructed by Claire Murphy and Dr. James McCarter at Washington University, St. Louis. The cDNA was made by using Dynabead oligo-dT priming (Dynal). PCR based library using a modified protocol from the SMART PCR cDNA Synthesis Kit from Clontech. Directionally cloned into the UDG sites of pAMP1. Nematodes were provided by Dr. Louis Gasbarree of the USDA, Beltsville, MD (lgasbarr@anri.barc.usda.gov).Third stage exsheathed larvae were collected from 14 day fecal-sphagnum moss cultures of Ostertagia eggs. The larvae were recovered by overnight passage on a Baermann apparatus, and then cleaned by passage through a 20 micron nylon mesh. The larvae were then subjected to a treatment with 1.25% chlorox to induce excystation. The larvae were washed with 5 changes of PBS and then pelleted and snap frozen in liquid nitrogen.

characteristic: description

characteristic: lab-host

text: Ostertagia ostertagi

text: NotI

text: SalI

text: Ostertagia ostertagi L3 pAMP1 v1

text: plasmid (ampicillin resistant)

text: pAMP1 (Gibco)


text: live

text: Generic

text: The library was constructed by Claire Murphy and Dr. James McCarter at Washington University, St. Louis. Oligo(dT)-SL1 PCR based library. Ostertagia ostertagi L4 cDNA PCR products of size >400 nucleotides containing SL1 on the 5' end and oligo(dT) on the 3' end were non-directionally cloned into pCRII-TOPO(Invitrogen) following the Topo TA cloning protocol. Nematodes were provided by Dr. Louis Gasbarree of the USDA, Beltsville, MD (lgasbarr@anri.barc.usda.gov). Late fourth stage larvae were recovered 10 days after infection of young calves with Ostertagia L3. The calves were killed and the abomasa (gastric glands) were recovered and washed extensively and then placed in aBaermann apparatus containing warm PBS for approximately 4 hours. The L4 that migrated from the tissues were washed extensively, pelleted and snap frozen in liquid nitrogen.

text: Ostertagia ostertagi

text: EcoRI

text: EcoRI

text: Ostertagia ostertagi L4 SL1 TOPO v1

text: plasmid (kanamycin and ampicillin resistant)

text: pCRII-TOPO (Invitrogen)


text: Royal Institute of Techology

text: live

text: Generic

text: Mus musculus

characteristic: strain

text: aorta

text: Endothelial gene expression related to shear stress


characteristic: External Id

text: Radiation Effects Research Foundation

text: live

text: Generic

text: Homo sapiens

text: thyroid

text: Homo sapiens thyroid


text: National Institute of Fruit Tree Science

characteristic: INSDC center name

text: live

text: Generic

text: Prunus persica

text: fruit

text: Prunus persica fruit


text: Max-Planck-Institute for Terrestrial Microbiology

characteristic: INSDC first public

text: live

text: Generic

text: presymbiotic hyphae

text: Gigaspora rosea

text: Gigaspora rosea presymbiotic hyphae


text: Kansas State University

text: live

text: Generic

text: Chinese spring

text: Total RNA was isolated from wheat tissues (leaves, anthers, ovaries) before anathesis and used for synthesis of differential expressed fragments among these tissues. Fragments that showed differential expression were recovered from gells, amplified, and cloned in pGM-T vector. Cloned cDNAs were sequenced with T7 or SP6 standard primers.

text: preanathesis

text: Triticum aestivum

text: Anthers

text: Differential display fragments A

text: pGM-T


text: Kansas State University

text: live

text: Generic

text: Chinese spring

text: Total RNA was isolated from wheat tissues (leaves, anthers, ovaries) before anathesis and used for synthesis of differential expressed cDNA fragments among these tissues. Fragments that showed differential expression were recovered from gells, amplified, and cloned in pGM-T vector. Cloned cDNAs were sequenced with T7 or SP6 standard primers.

text: preanathesis

text: Triticum aestivum

text: Ovaries

text: Differential display fragments O

text: pGM-T


text: The University of Tokyo, Human Genome Center

text: live

text: Generic

text: Homo sapiens

text: vestibule

text: human vestibular cDNA library


text: Graduate School of Engineering, Osaka University

text: live

text: Generic

text: lactating female

text: Rattus norvegicus

text: female

characteristic: sex

text: brain

text: Rattus norvegicus brain lactating female


characteristic: INSDC last update

text: live

text: Generic

text: lactating adult

text: Rattus norvegicus

text: female

text: Rattus norvegicus lactating adult


text: University of Toronto

text: live

text: Generic

text: Smooth muscle cells (Clonetics, Walkersville, MD)

characteristic: cell type

text: Primary cell cultures

characteristic: cell-line

text: Confluent human carotid artery smooth muscle cells were made quiescent by culturing in media without growth supplements for 48 h. The cells were then stimulated with media containing FBS, insulin, EGF, FGF-B and PDGF-B for 0, 3 and 8 h. Total RNA was isolated and subjected to differential display/RT-PCR according to Liang, P. & Pardee, A.B. (1992, Science 257: 967-971) using one anchored oligo-dT primer labeled with a green fluorophore (FH-T11G) and six 13-mer arbitrary primers. The primers were tailed with HindIII sites. Differential display bands were recovered from sequencing gel and subcloned to the HindIII site in PCR-TRAP vector.

text: Passages 5-7

text: Homo sapiens

text: HindIII

text: HindIII

text: Carotid artery

characteristic: tissue-type

text: Human growth factor stimulated vascular smooth muscle cells, DD/RT-PCR products

text: Plasmid

text: PCR-TRAP vector (GenHunter, Nashville, TN)


text: Centers for Disease Control and Prevention

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Cervical keratinocytes

text: Subclone 20861 of W12 cell line

text: Genes differentially expressed during keratinocyte differentiation were identified by comparing DD-PCR profiles of cells grown under non-differentiating (monolayers) and differentiating (raft cultures) conditions. Differentially expressed bands were excised and sequenced for functional annotation

text: Homo sapiens

text: Human cervical cDNA (differential display PCR)


text: Xijing Hospital, Fourth Military Medical University

text: live

text: Generic

text: Homo sapiens

characteristic: organism

text: Colorectal Carcinoma

text: Human SW480 Lambda gt11 cDNA Library

characteristic: title


text: Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem

text: live

text: Generic

text: Young open flower at stage four

characteristic: dev-stage

text: Rosa hybrid cultivar

text: EcoRI

text: XhoI

text: Fragrant Cloud

text: Petals

text: Rose Petals (Fragrant Cloud) Lambda Zap Express Library

text: Phagemid

text: pBKCMV

characteristic: vector


text: live

text: Generic

text: Young open flower at stage four

text: Rosa hybrid cultivar

text: EcoRI

text: XhoI

text: Golden Gate

text: Petals

text: Rose Petals (Golden Gate) Lambda Zap Express Library

text: Phagemid

text: pBKCMV


text: Institut National de la Recherche Agronomique

text: live

text: Generic

text: Cabernet sauvignon

characteristic: cultivar

text: Roots originating from In vitro culture

characteristic: description

text: In vitro culture

text: root

characteristic: organ

text: Vitis vinifera

text: Grape cDNA library

text: plasmid

text: pGEM-T


characteristic: External Id

text: Agriculture & Agri-Food Canada

characteristic: INSDC center name

text: live

text: Generic

text: Developing microsclerotia (DMS) cDNA library; constructed from axenic cultures grown on cellulose membranes overlaid onto a basal medium agar. Cells were harvested at 4 days post-inoculation. Single-pass sequencing was done using the T3 promoter primer: 5' ATTAACCCTCACTAAAGGGA 3'.

text: E. coli

characteristic: lab-host

text: Verticillium dahliae

text: EcoRI

text: XhoI

characteristic: strain

text: phagemid

text: pBluescript SK-


text: Agriculture & Agri-Food Canada

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Simulated xylem fluid (SXM) cDNA library; constructed from axenic cultures grown in a liquid simulated xylem fluid medium. Cells were harvested at 4 days post-inoculation. Single-pass sequencing was done using the T3 promoter primer: 5' ATTAACCCTCACTAAAGGGA 3'.

text: Verticillium dahliae

text: EcoRI

text: XhoI

text: spores/mycelia

text: phagemid

text: pBluescript SK(-)


text: Corixa Corporation

text: live

text: Generic

text: Tester cDNA was made from three breast tumor samples and driver cDNA was synthesized from a pool of normal tissues including breast, brain, liver, pancreas, and peripheral blood mononuclear cells. The subtraction was performed using Clontech's PCR-Select cDNA Subtraction kit. The PCR products of the subtracted cDNAs were subcloned into pCR2.1-TOPO TA cloning vector.

text: Breast

text: Homo sapiens

text: EcoRI

text: EcoRI

text: Female

characteristic: sex

text: Breast Tumor

characteristic: tissue-type

text: pCR2.1-TOPO (Invitrogen)


text: The Institute for Genomic Research (TIGR; www.tigr.org)

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Kennebec or Binjte

text: supplier: Combination of untreated and Phytophthora infestans-treated libraries of stolons, leaves, leaflets, axillary buds of stem explants, petioles, germinating eyes, tubers, or roots.

text: SOLR

text: Solanum tuberosum

text: EcoRI

text: XhoI

text: mixed tissues

text: mixed potato tissues

text: pBluescript SK(-)


text: Baylor College of Medicine

text: live

text: Generic

characteristic: NCBI submission package

text: Immature seeds, collected from pods ranging in age from 25 to 35 days after pollination, were harvested from greenhouse-grown plants. Seed were removed and separated from pod walls and immediately frozen in liquid nitrogen. Seeds throughout the age range were pooled for mRNA extraction. cDNA was prepared from polyA+ enriched RNA. The cDNA was directionally ligated into the Unizap XR vector from Stratagene and packaged using Gigapack III Gold packaging extracts. Plasmids containing cDNA inserts were excised from the recombinant lambda-Zap phage using Ex-assist helper phage and propagated in XLOLR cells.

text: 25 to 35 days after pollination

text: XLOLR

text: Medicago truncatula

characteristic: organism

text: EcoRI

text: XhoI

text: Immature seeds

text: GLSD

text: phagemid

text: pBluescript SK-


text: Genoscope - Centre National de Sequencage

text: live

text: Generic

text: subtracted cDNA library

text: embryonic

characteristic: dev-stage

text: Danio rerio

text: inner ear

text: Danio rerio embryonic inner ear subtracted cDNA

characteristic: title


text: Washington University School of Medicine

text: live

text: Generic

text: The library was constructed by Claire Murphy and Dr. James McCarter at Washington University, St. Louis. Oligo(dT)-SL1 PCR based library. Adult Toxocara canis cDNA PCR products of size >400 nucleotides containing SL1 on the 5' end and oligo(dT) on the 3' end were non-directionally cloned into pCRII-TOPO(Invitrogen) following the Topo TA cloning protocol. Adult nematodes were collected from infected dogs and provided by Dr. Prema Arasu of North Carolina State University, Raleigh, NC (Prema_Arasu@ncsu.edu).

text: Adult

text: Toxocara canis

text: EcoRI

text: EcoRI

text: Toxocara canis adult SL1 TOPO v1

text: plasmid (kanamycin and ampicillin resistant)

text: pCRII-TOPO (Invitrogen)

characteristic: vector


text: live

text: Generic

text: The library was constructed by Claire Murphy and Dr. James McCarter at Washington University, St. Louis. Oligo(dT)-SL1 PCR based library. Meloidogyne hapla egg cDNA PCR products of size >400 nucleotides containing SL1 on the 5' end and oligo(dT) on the 3' end were non-directionally cloned into pCRII-TOPO(Invitrogen) following the Topo TA cloning protocol. Eggs were provided by Dr. David Bird of North Carolina State University, Raleigh, NC (david_bird@ncsu.edu)

characteristic: description

text: eggs

text: Meloidogyne hapla

text: EcoRI

text: EcoRI

text: Meloidogyne hapla egg SL1 TOPO v1

text: pCRII-TOPO (Invitrogen)


text: John Innes Centre

text: live

text: Generic

text: Chinese spring

characteristic: cultivar

text: Triticum aestivum

text: leaf

text: Triticum aestivum cv. Chinese spring leaf


characteristic: External Id

text: live

text: Generic

text: Stark Early Orange

text: Shoot bursting, post-dormancy

text: leaf

characteristic: organ

text: Prunus armeniaca

text: Apricot cDNA library

text: plasmid

text: pGEM-T


text: Henan Normal Univ

characteristic: INSDC center name

text: live

text: Generic

text: Rattus norvegicus

text: SD rat

text: liver

text: SD rat liver


text: GSF - Forschungszentrum, Institut fuer Molekulare Strahlenbiologie

text: live

text: Generic

text: bursal lymphocyte

characteristic: cell type

text: CB inbred strain

text: 2-3 weeks old

text: Gallus gallus


text: The University of Georgia, Department of Plant Biology

characteristic: INSDC first public

text: live

text: Generic

text: The library was made from trophont polyA+ RNA of the G5 parasite strain. Double-stranded cDNA was linked to EcoRI adaptors, size fractionated, and material >500 bp cloned into lambda ZAP II. Plasmid DNA for sequencing was prepared by mass excision.

text: Ichthyophthirius multifiliis

text: EcoRI

text: EcoRI

characteristic: strain

text: G5 trophont cDNA (INIT1)

text: Plasmid

text: pBluescript SK(-) from Lambda Zap II


text: Tianjin Institute of Urologic Surgery

characteristic: INSDC last update

text: live

text: Generic

text: Poly A+ RNA were isolated from tissues of bladder transitional cell carcinoma and normal bladder epithelia tissues respectively. Then double-strand cDNA were synthesized and restricted by Rsa I. The carcinoma cDNA were divided into two groups and ligated with either adaptor 1 or adaptor 2. After hybridizing with normal cDNA twice and undergoing nested PCR, the PCR products were cloned into PT-Adv vector and transformed E.Coli TOP10F'.

text: T1 period of bladder transitional cell carcinoma

text: bladder

text: Homo sapiens

text: male

characteristic: sex

text: epithelia

text: subtractive library of bladder carcinoma

text: Plasmid

text: PT-Adv


text: Henan Normal Univ

text: live

characteristic: INSDC status

text: Generic

text: Rattus norvegicus

text: liver

characteristic: tissue-type

text: Rat liver (Lee,Y.)


text: UNL, East Campus

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Mus musculus

text: male

text: hypothalamic tissue

text: Pomp Mouse Hypothalamus


text: INSERM U433 , Faculte Medecine Laennec

text: live

text: Generic

text: Mus musculus

characteristic: organism

text: female

text: Swiss

text: hypothalamus

text: Mus musculus hypothalamus Swiss


text: college of life sciences

text: live

text: Generic

text: Oryza sativa

text: seed

text: rice seed cDNA library

characteristic: title


text: Universtiy of Maryland

text: live

text: Generic

text: Metarhizium anisopliae sf. acridum was grwon on insect cuticle and chitin for 24 hours. A cDNA library was constructed in the unidirectional Lambda vector UniZap.

text: Metarhizium acridum

text: ARSEF 324

text: Metarhizium anisopliae sf. acridum ARSEF 324

text: UniZap

characteristic: vector


text: Nanfan Hospital

text: live

text: Generic

text: stem cell

text: the products of SSH(suppression subtractive hybridization) are inserted into T/A vector

characteristic: description

text: adult

characteristic: dev-stage

text: Rattus norvegicus

text: female

text: Sprague-Dawley

text: hematopoiesis

text: Hematopoietic Stem Cell Library


characteristic: External Id

text: Chinese Medical University

text: live

text: Generic

text: adult

text: stomach

text: Homo sapiens

text: male

text: cDNA subtractive library of human gastric cancer


text: University of Minnesota

text: live

text: Generic

characteristic: cultivar

text: cDNA was prepared from polyA+ enriched RNA. The cDNA was directionally ligated into the Unizap XR vector from Stratagene and packaged using Gigapack III Gold packaging extracts. Plasmids containing cDNA inserts were excised from the recombinant lambda-Zap phage using Ex-assist helper phage and propagated in XLOLR cells.

text: various stages

text: XLOLR

characteristic: lab-host

text: Medicago truncatula

text: EcoRI

text: XhoI

text: mixed tissues

text: KVKC

text: phagemid

text: pBluescript SK-


text: Nara Institute of Science and Technology

characteristic: INSDC center name

text: live

text: Generic

text: Mus musculus

text: brain

text: 3'-directed mouse cDNA library


text: Inra

characteristic: INSDC first public

text: live

text: Generic

text: seedling

text: Pinus pinaster

text: aerial part

text: Pinus pinaster aerial part seedling


text: Inra

text: live

text: Generic

text: seedling

text: Pinus pinaster

text: needles

text: Pinus pinaster needles seedling


text: Japan Science and Technology Corporation

text: live

text: Generic

text: stage 24 (16 somite stage)

text: Oryzias latipes

text: CAB

characteristic: strain


text: UFMG

characteristic: INSDC last update

text: live

text: Generic

text: Triatoma brasiliensis

text: salivary glands

text: T. brasiliensis salivary glands cDNA library


text: The Samuel Roberts Noble Foundation

text: live

text: Generic

text: Young trifoliate leaves of Medicago truncatula were excised and dip-inoculated in a spore suspension of Phoma medicaginis, and incubated in humid dishes. Pools of leaves were harvested at 0, 15, and 30 minutes and 1, 2, 3, 6, 14, 24, 48, 72, and 96, hours, and used to prepare total RNA. cDNA was prepared from polyA+ enriched, pooled samples of equivalent amounts of total RNA from each sample. The cDNA was directionally ligated into the Uni-Zap XR vector (Stratagene) and packaged using the Gigapack III Gold packaging extracts. Phagemids containing cDNA inserts were in vivo excised from the recombinant Uni-ZAP XR vector using ExAssist helper phage and the E. coli strain XL1-Blue MRF' (Stratagene). Excised plasmids were plated using SOLR cells.

text: Pathogen-induced, young trifoliate

text: Medicago truncatula

text: leaf

text: Phoma-infected

text: Plasmid

text: pBluescript SK(-)


text: National Institute of Mental Health

text: live

text: Generic

text: UI-M-DJ2 is a subtracted cDNA library constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is: GCTACATGAT, subfornical organ and area postrema.

text: Adult

text: DH10B (Life Technologies) (T1 phage resistant)

text: brain

characteristic: organ

text: Mus musculus

text: EcoR I

text: Not I

text: subfornical organ and postrema

text: Plasmid

text: pT7T3-Pac (Pharmacia) with a modified polylinker


text: live

characteristic: INSDC status

text: Generic

text: The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. Denatured mRNa was size fractionated on a 1% agarose gel. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was size selected according to mRNA size fraction, ligated with EcoR I adaptor, digested with Not I, and then cloned directionally into pYX-Asc vector. The library tag sequence located between the Not I site and the polyA tail, is GTGCGTGGAA. This library was created for the University of Iowa Mouse Brain Molecular Anatomy Project (BMAP): 'Gene Discovery in the Developing Mouse Nervous System', supported by National Instututes of Mental Health (NIMH), Hemin Chin, Ph.D., program coordinator.

text: embryo 15.5 dpc

text: DH10B (T1 phage resistant)

text: brain

text: Mus musculus

text: EcoR I

text: Not I

text: whole brain

text: Plasmid

text: pYX-Asc


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.77 kb. Library constructed by Life Technologies. Note: this is a NCI_CGAP Library.

text: DH10B (T1-resistant)

text: stomach

text: Mus musculus

text: SalI

text: NotI

text: plasmid (ampicillin resistant)


text: Institute of Genetics, Biological Research Center

text: live

text: Generic

text: Medicago sativa

text: Medicago sativa library (Kalo P)


text: Institute of Molecular Biology and Genetics

text: live

text: Generic

text: E.Coli

text: Brain

text: Oncorhynchus keta

text: EcoRI

text: EcoRI

text: Chum Salmon Brain cDNA Library

text: Phage

text: Lambda Zap


text: National Cancer Institute / NIH

text: live

text: Generic

text: UI-R-DZ1 is a normalized cDNA library containing the following tissue(s): Swarm Rat Chondrosarcoma. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CATTCTTGTA. The Rat cartilaginous tumor tissue was provided by Dr Jeff Stevens at the University of Iowa.

text: 37 days

text: DH10B (Life Technologies)

text: Spine

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Chondrosarcoma

characteristic: tissue-type

text: Phagemid


text: Children's Memorial Research Center

text: live

text: Generic

characteristic: NCBI submission model

text: UI-R-EP0 is a subtracted cDNA library constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tags for this library are: distal colon, GAAGTGCTCC; osteoblast, AAGATATCAA; cell line R3327 5A, GGACTAGATC; cell line R3327 5P, CACGTGAGAT; duodenum, TGTGGTTCAT; prostate, CCAGG.

text: ADULT

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid


text: University of Georgia

text: live

text: Generic

characteristic: NCBI submission package

text: isolate=SES-3; grown with selenate as electron acceptor

text: Sulfurospirillum barnesii

text: Sulfurospirillum barnesii isolate SES-3


text: Tsinghua University

text: live

text: Generic

text: induced by salicylic acid

text: Gossypium hirsutum

characteristic: organism

text: cotyledon

text: Gossypium hirsutum cotyledon


text: Peking University

text: live

text: Generic

text: Homo sapiens

text: upregulated by angiotensin II in mesangial cells

characteristic: title


text: Cereal Research Centre, Agriculture and Agri-food Canada

text: live

text: Generic

text: Glenlea

text: mRNA obtained from wheat seeds of cultivar Glenlea 5 days post-anthesis

text: 5 days after anthesis

text: E. coli DH10B

text: Triticum aestivum

text: NotI

text: MluI

text: developing seeds

text: pSPORT-P (Invitrogen Technologies)

characteristic: vector


text: live

text: Generic

text: Glenlea

text: mRNA obtained from wheat seeds of cultivar Glenlea 15 days post-anthesis

characteristic: description

text: 15 days after anthesis

text: E. coli DH10B

text: Triticum aestivum

text: NotI

text: MluI

text: developing seeds

text: pCMV-SPORT6.0 (Invitrogen Technologies)


text: live

text: Generic

text: Glenlea

text: mRNA obtained from wheat seeds of cultivar Glenlea 25 days post-anthesis. ***NOTE: it was later determined that the library was contaminated with human sequences

text: 25 days after anthesis

characteristic: dev-stage

text: E. coli DH10B

text: Triticum aestivum

text: NotI

text: MluI

text: developing seeds

text: pCMV-SPORT6.0 (invitrogen Technologies)


characteristic: External Id

text: Harvard University

text: live

text: Generic

text: ZAP-cDNA library,Stratagene (cat. #200400 and #200450)

text: XL1-Blue MRF' cells

text: Pyrocystis lunula

text: CCMP 731

text: P.lunula ZAP-cDNA library

text: phagemid

text: pBluescript SK


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: cDNA made by oligo-dT priming. Directionally cloned into EcoRI/XhoI sites using the following 5' adaptor: GGCACGAG(G). Library constructed by Ling Hong in the laboratory of Gerald M. Rubin (University of California, Berkeley) using ZAP-cDNA synthesis kit (Stratagene) and Superscript II RT (Life Technologies). Note: this is a NIH_MGC Library.

text: DH10B (phage-resistant)

text: prostate

text: Homo sapiens

text: EcoRI

text: XhoI

text: carcinoma, cell line

text: plasmid (chloramphenicol resistant, grown at 30C)


text: live

text: Generic

text: Double-stranded cDNA was prepared from a pool of 40 cell line polyA+ RNAs (bladder - 2%, blood - 33.4%, brain - 5.6%, breast - 12.5%, colon - 4%, connective tissue - 1.4%, eye - 1%, intestine - 2.6%, kidnney - 2.2%, liver - 5.7%, lung - 10.8%, NK-cell - 5.2%, ovary - 4%, pharynx - 2.5%, prostate - 4.3%, salivary gland - 1.3%, and skin - 2.3%). 5' and 3' adaptors were used in cloning as follows: 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' and 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)NN-3'. Full-length enriched library was constructed using the Clontech Creator SMART kit and size-selected to contain the 0.5-1 kb size fraction (other fractions present in NIH_MGC_127 and NIH_MGC_128). Library created in the laboratory of T. Usdin, M.D., Ph.D. (NIMH, NIH). Note: this is a NIH_MGC Library.

text: DH10B (T1-phage-resistant)

text: Homo sapiens

text: SfiI (ggccattatggcc)

text: SfiI (ggccgcctcggcc)

text: mixed (pool of 40 RNAs)

text: pDNR-LIB


text: live

text: Generic

text: Double-stranded cDNA was prepared from a pool of 40 cell line polyA+ RNAs (bladder - 2%, blood - 33.4%, brain - 5.6%, breast - 12.5%, colon - 4%, connective tissue - 1.4%, eye - 1%, intestine - 2.6%, kidnney - 2.2%, liver - 5.7%, lung - 10.8%, NK-cell - 5.2%, ovary - 4%, pharynx - 2.5%, prostate - 4.3%, salivary gland - 1.3%, and skin - 2.3%). 5' and 3' adaptors were used in cloning as follows: 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' and 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)NN-3'. Full-length enriched library was constructed using the Clontech Creator SMART kit and size-selected to contain the 1-2 kb size fraction (other fractions present in NIH_MGC_126 and NIH_MGC_128). Library created in the laboratory of T. Usdin, M.D., Ph.D. (NIMH, NIH). Note: this is a NIH_MGC Library.

text: DH10B (T1-phage-resistant)

text: Homo sapiens

text: SfiI (ggccattatggcc)

text: SfiI (ggccgcctcggcc)

text: mixed (pool of 40 RNAs)

text: pDNR-LIB


text: live

text: Generic

text: DH10B (phage-resistant)

text: ovary

text: Homo sapiens

text: EcoRI

text: XhoI

text: teratocarcinoma, cell line


text: live

text: Generic

text: Double-stranded cDNA was prepared from a pool of 40 cell line polyA+ RNAs (bladder - 2%, blood - 33.4%, brain - 5.6%, breast - 12.5%, colon - 4%, connective tissue - 1.4%, eye - 1%, intestine - 2.6%, kidnney - 2.2%, liver - 5.7%, lung - 10.8%, NK-cell - 5.2%, ovary - 4%, pharynx - 2.5%, prostate - 4.3%, salivary gland - 1.3%, and skin - 2.3%). 5' and 3' adaptors were used in cloning as follows: 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' and 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)NN-3'. Full-length enriched library was constructed using the Clontech Creator SMART kit and size-selected to contain the >2 kb size fraction (other fractions present in NIH_MGC_126 and NIH_MGC_127). Library created in the laboratory of T. Usdin, M.D., Ph.D. (NIMH, NIH). Note: this is a NIH_MGC Library.

text: DH10B (T1-phage-resistant)

text: Homo sapiens

text: SfiI (ggccattatggcc)

text: SfiI (ggccgcctcggcc)

text: mixed (pool of 40 RNAs)

text: pDNR-LIB


text: live

text: Generic

text: DH10B (phage-resistant)

text: breast

text: Homo sapiens

text: EcoRI

text: XhoI

text: adenocarcinoma, cell line


text: live

text: Generic

text: cDNA made by oligo-dT priming. Directionally cloned into SalI/NotI sites using the following 5' adaptor: 5'-TCGACCCACGCGTCCG-3'. Size-selected for average insert size 1.8-1.9 kb. Library constructed by J. Baker (Stanford University).

text: embryo, 6.5 dpc

text: Mus musculus

text: NotI

text: SalI

text: embryo, early gastrula

text: Baker mouse embryo e6.5

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: embryo, 7.5 dpc

text: Mus musculus

text: NotI

text: SalI

text: embryo, late gastrula

text: Baker mouse embryo e7.5

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: mRNA made from flow-sorted, bone marrow progenitors, cDNA made by oligo-dT priming. Directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 300 bp. Primary library. cDNA Library Preparation: David B. Krizman, Ph.D. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.

text: blood

text: Mus musculus

text: flow-sorted, bone marrow progenitors

text: plasmid


text: Washington University School of Medicine

text: live

text: Generic

text: SL1-PCR-TOPO based library. Ancylostoma ceylanicum cDNA were PCR amplified, using SL1-EcoRI primer on the 5' end and oligo(dT)-XhoI on 3' end, were gel fractionated, and non-directionally cloned into pCR-TOPO-XL (Invitrogen), following XL-Topo TA cloning protocol. The cDNA insert can be excised with EcoRI. The library was constructed by Adia Magalska and Dr. Vadim Kapulkin at the Department of Infectious Diseasaes, Microbiology and Immunology of Veterinary Faculty at the Warsaw Agricultural University. The strain used in this work was provided by Prof. Halina Wedrychowicz.

text: pre-infective L3

text: Ancylostoma ceylanicum

text: EcoRI

text: EcoRI

text: mixed

characteristic: sex

text: whole organism

text: Vadim Kapulkin Ancylostoma ceylanicum SL1-PCR

text: plasmid

text: pCR-TOPO-XL (KAN resistant)


text: The Sanger Centre

text: live

text: Generic

text: Homo sapiens


text: Anhui Medical University

text: live

text: Generic

text: larva worms

text: Schistosoma japonicum

text: mixed

text: Chinese strain

text: cDNA larva worms of Schistosoma japonicum


text: Institute of Zoology, The Chinese Academy of Sciences

text: live

text: Generic

text: The genes critical to embyro implantation in rhesus monkey were screened by Suppression Subtractive Hybridization methods.The dscDNA of endometrium from implantation site was the tester, while that from non-implantation site was the driver.

text: About the 9th day after fertilization

characteristic: lab-host

text: Macaca mulatta

text: female

text: Endometrium

text: Subtracted library of Rhesus monkey endometrium

text: plasmid

text: PGEM-T Easy vector(Promega)


text: US Department of Agriculture, Agriculture Research Service, Pacific West Area, Western Regional Research Center

characteristic: INSDC center name

text: live

text: Generic

text: Chinese Spring

characteristic: cultivar

text: Seeds were surface-sterilized,germinated and grown aseptically in the dark at room temperature on filter paper with water,nystatin and cefotaxime in covered crystallization dishes. Five-day old seedlings were transferred to 5 C cold room and kept for 48 hr. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made,and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi,Close,Fenton) at the University of California,Riverside. The cDNA clones were in vivo excised to give pBluescript phagemids before subtraction was carried out. The mass excision of phagemid library and subtraction were done in HT Nguyen lab by D. Zhang at Texas Tech University. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

text: Five-day old seedling

text: E. coli SOLR

text: Triticum aestivum

text: EcoRI

text: XhoI

text: Seedling

text: Wheat cold-stressed seedling subtracted cDNA library

text: Phagemid

text: Lambda Uni-ZAP XR, excised phagemid


text: Imperial College of Science, Technology and Medicine

characteristic: INSDC first public

text: live

text: Generic

text: mycelium

characteristic: cell type

text: cDNA of mycelium, 3d after infection, in Clontech vector lambda TriplEx2. Sorted library.

text: 3d after infection

text: Hordeum vulgare

text: Blumeria hordei

characteristic: strain

text: Bgh mycelium lambda TriplEx2

text: lambda TriplEx2


text: Cancer Institute

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: squamous cell carcinoma

text: Homo sapiens

text: lung

text: Human lung squamous cell carcinoma


text: USDA-ARS

text: live

text: Generic

characteristic: NCBI submission model

text: Chinese Spring

text: Triticum aestivum

text: immature spikes

characteristic: tissue-type

text: Triticum aestivum immature spikes


text: Shanghai Institute of Endocrinology, Ruijin Hospital, Shanghai Second Medical University

text: live

text: Generic

characteristic: NCBI submission package

text: one year

text: fat

characteristic: organ

text: Rattus norvegicus

characteristic: organism

text: male

text: Sprague-Dawley

text: adipose tissue

text: Rat adipose tissue cDNA Library


text: live

text: Generic

text: one year

text: kidney

text: Rattus norvegicus

text: male

text: Sprague-Dawley

text: renal tissue

text: Rat renal tissue cDNA Library

characteristic: title


text: Bar-Ilan University

text: live

text: Generic

text: hypocotyl

text: Cucumis melo

text: Melon seedling hypocotyl cDNA library

text: lambda Zap II

characteristic: vector


characteristic: External Id

text: Bar-Ilan University

text: live

text: Generic

text: leaf

text: Cucumis melo

text: Melon RT-PCR reactions


text: Jiang Xi Medical College

text: live

text: Generic

text: These sequences were screened by cockroach sensitive patients' IgG4 serum

characteristic: description

text: nymph

characteristic: dev-stage

text: E.coli NM522

text: Periplaneta americana

text: whole body

text: Periplaneta americana Lambda Express library

text: Phage

text: Lambda Excell


characteristic: INSDC center name

text: live

text: Generic

text: Chinese Spring

text: Plants were grown under hydroponic conditions for four days. Root tips were excised and snap frozen (Ross and Gustafson) and total RNA was prepared at University of Missouri, Columbia. Poly(A) RNA was purified, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript SK(-)phagemids in the TJ Close lab (Chin and Close) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

text: Four-day old seedling

text: E. coli SOLR

characteristic: lab-host

text: Triticum aestivum

text: EcoRI

text: XhoI

text: Root tip at 1.0 to 1.5 mm stage

text: Wheat unstressed root tip cDNA library

text: Phagemid

text: Lambda Uni-ZAP XR, excised phagemid


text: Ludwig Institute for Cancer Research

characteristic: INSDC first public

text: live

text: Generic

text: A mini-library was made by cloning products derived from ORESTES PCR (U.S. Letters Patent application No. 196,716 - Ludwig Institute for Cancer Research) profiles into the pUC 18 vector. Reverse transcription of tissue mRNA and cDNA amplification were performed under low stringency conditions.

text: Adult

text: breast

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: Ludwig Institute for Cancer Research

text: live

text: Generic

text: Adult

text: colon

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: Ludwig Institute for Cancer Research

text: live

text: Generic

text: Adult

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: Ludwig Institute for Cancer Research

text: live

text: Generic

text: Adult

text: marrow

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: Ludwig Institute for Cancer Research

text: live

text: Generic

text: Adult

text: ovary

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: UT M.D. Anderson Cancer Center

text: live

text: Generic

text: Homo sapiens

text: Differential display of retinoic acid receptor beta transfected and non-transfected cells


text: University of Helsinki

text: live

text: Generic

text: dry embryo

text: embryo

text: Hordeum vulgare


text: University of Helsinki

text: live

text: Generic

text: leaf C19819 85/1, 85/2

text: Hordeum vulgare

text: leaf


text: University of Helsinki

text: live

text: Generic

text: 2-,3-,4-days after germination

text: Hordeum vulgare

text: Root


text: University of Helsinki

text: live

text: Generic

text: 2-,3-,4-days after germination

text: Hordeum vulgare

text: Shoot


text: University of Helsinki

text: live

text: Generic

text: 2-,3-,4-days after germination

text: Hordeum vulgare

text: Aleurone


text: University of Maryland School of Medicine

text: live

text: Generic

text: Subtracted cDNA subjected to nested PCR amplification and cloned into pGMT-vector.

text: Dermacentor variabilis

text: laboratory strain

text: Ovary

text: Plasmid subtracted Dermacentor variabilis ovary library


text: University of Helsinki

text: live

text: Generic

text: Callus K19

text: Hordeum vulgare

text: Callus


text: University of Helsinki

text: live

text: Generic

text: 0-,3-,6-,9-days after pollination

text: Hordeum vulgare

text: Developing seed


text: INIA - Carillanca

text: live

text: Generic

text: Victoria Baer

characteristic: cultivar

text: Total RNA was isolated from phosphate-starved and phosphate-starved rescued proteoid root clusters, 24 and 72 h after supplying 0.1 mM phosphate to 24 days phosphate starved plants. Complementary DNA was synthesized from mRNA using a one-base anchored poly(dT)and amplified by PCR in the presence of the anchor primer in combination with an arbitrary primer, both with a HindIII restriction site at their 5'end. DNAs were visualized by differential display and the differentially expressed fragments were recovered, amplified and cloned into pGEM-T vector. Cloned cDNAs were sequenced with M13 universal primers.

text: E. coli JM109

text: Lupinus albus

text: proteoid root clusters

text: Differential display fragments

text: plasmid

text: pGEM-T


text: Stanford University

text: live

text: Generic

text: Unamplified cDNA library directionally cloned by Rima Kulikauskas using Stratagene's Uni-Zap system. Insert sizes ranged from 0.5Kb to 2Kb. 50 microliter aliquot had 338,000 pfu when it was made in Sept, 1995, from oligo dT-primed poly A+ RNA.

text: mature

text: SOLR

text: Zea mays

text: EcoRI (5-prime)

text: XhoI (3-prime)

text: pollen

text: 3524 - Mature pollen from Sheila McCormick's lab

text: phagemid

text: Stratagene's Uni-Zap XR (pBluescript SK-)


text: Friedrich Miescher-Institut

text: live

text: Generic

text: Solanum tuberosum

text: Phytophthora infestans

text: Mycelium

text: Phytophthora infestans Mycelium


text: live

text: Generic

text: The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. Denatured mRNa was size fractionated on a 1% agarose gel. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was size selected according to mRNA size fraction, ligated with EcoR I adaptor, digested with Not I, and then cloned directionally into pYX-Asc vector. The library tag sequence located between the Not I site and the polyA tail, is TGAGAGAGCC. This library was created for the University of Iowa Mouse Brain Molecular Anatomy Project (BMAP): 'Gene Discovery in the Developing Mouse Nervous System', supported by National Instututes of Mental Health (NIMH), Hemin Chin, Ph.D., program coordinator.

text: embryo 12.5 dpc

text: DH10B (T1 phage resistant)

text: brain

text: Mus musculus

text: EcoR I

text: Not I

characteristic: strain

text: whole brain

text: Plasmid

text: pYX-Asc


characteristic: INSDC last update

text: live

text: Generic

text: NCI_CGAP_Ct1 is a normalized cDNA library containing the following tissue(s): Osteoarthritic Cartilage The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is TGATCACGCT.

text: Adult

text: DH10B (Life Technologies)

text: Knee

text: Homo sapiens

text: EcoR I

text: Not I

text: Osteoarthritic Cartilage

text: Plasmid

text: pT7T3-Pac (Pharmacia) with a modified polylinker


text: Washington University School of Medicine

text: live

characteristic: INSDC status

text: Generic

text: The library was constructed by Claire Murphy and Dr. James McCarter at Washington University, St. Louis. The cDNA was made by using Dynabead oligo-dT priming (Dynal). PCR based library using a modified protocol from the SMART PCR cDNA Synthesis Kit from Clontech. Directionally cloned into the UDG sites of pAMP1. Adult nematodes were harvested from infected dogs by Dr. Prema Arasu of North Carolina State University, Raleigh, NC (Prema_Arasu@ncsu.edu). Total RNA was isolated by Merry McLaird of Divergence, Inc., St. Louis,MO.

text: adult

text: Dirofilaria immitis

text: NotI

text: SalI

text: Dirofilaria immitis adult pAMP1 v1

text: plasmid (ampicillin resistant)

text: pAMP1 (Gibco)


text: Illinois State University

text: live

text: Generic

characteristic: NCBI submission model

text: Library provided by Dr. Philip LoVerde, SUNY, Buffalo. directionally cloned cDNAs

text: Golden Hamster

text: Schistosoma mansoni

text: EcoRI

text: XhoI

text: mixed sex adult

characteristic: sex

text: Puerto Rican

text: Schistosoma mansoni adult, Phil LoVerde/Joe Merrick

text: Lambda ZAPII


text: Leibniz Institute of Plant Genetics and Crop Plant Research (IPK)

text: live

text: Generic

characteristic: NCBI submission package

text: Barke

text: 0-7 DAP(days after pollination) Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable. Average insert size is 1 kb

text: 0-7 DAP (days after pollination)

text: Hordeum vulgare subsp. vulgare

text: EcoRI (5'-end of cDNA)

text: XhoI (3'-end of cDNA)

text: embryosac

characteristic: tissue-type

text: HA

text: pBluescript SK+


text: Institute of Plant Genetics and Crop Plant Research (IPK)

text: live

text: Generic

text: Barke

text: Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.

text: 0-16 hours after imbibition

text: Hordeum vulgare subsp. vulgare

characteristic: organism

text: EcoRI (5'-end of cDNA)

text: XhoI (3'-end of cDNA)

text: embryo + scutellum

text: HS

text: pBluescript SK+