text: ISV-CNRS

text: live

text: Generic

text: Physiological condition: greenhouse plants. tissue_type: Green siliques

text: Arabidopsis thaliana

text: Gif-SeedA

text: Lambda ZAPII non-oriented

characteristic: vector


status: PUBLIC

characteristic: External Id

text: Biologie Moleculaire des Plantes - CNRS

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Physiological condition: leaves strips incubated 2/3/4 days in liquid culture medium. tissue_type: sliced leaves

characteristic: description

text: Arabidopsis thaliana

characteristic: organism

text: ecotype columbia

tag: attribute

characteristic: strain

text: Strasbourg-FA

characteristic: title

text: Lambda ZAPII


text: University of Minnesota

text: live

text: Generic

text: mRNA was purified from macrophages derived from bone marrow cells using recombinant murine granulocyte/macrophage colony stimulating factor (rmGM-CSF). Half of the macrophage population was activated by gamma interferon (IFN-gamma). The resulting two cDNA libraries (with and without IFN-gamma stimulation) were subtracted in the way that only messages induced by IFN-gamma are saved to make a subtraction cDNA library according to the method of Herfort and Garber (BioTech.(1991) 11:598-606).

characteristic: lab-host

text: Mus musculus

text: EcoRI

text: EcoRI

text: C57BL/6J x C3H/HeJ F1

text: Subtraction cDNA library of GM-CSF-derived macrophages from GM-CSF-derived, gamma interferon-activated macrophages

text: pBluescript SK-


text: California State University

text: live

text: Generic

text: mRNA isolated from illuminated leaves and sheaths of 5 week old plant. cDNA directionally cloned into vector.

text: Zea mays

text: Maize Leaf, Stratagene #937005

text: Phage

text: Uni-ZAP


text: Massachusetts General Hospital

text: live

text: Generic

text: Exons were isolated from human chromosome 9 specific cosmids using a modification of the method of exon amplification (Proc. Natl. Acad. Sci. USA 88:4005-4009, 1991). Amplified exons were digested with SalI and BamHI and subsequently cloned into pBluescriptIIKS+ at the SalI and BglII sites.

text: E. coli DH5a

text: Homo sapiens

text: Chromosome 9 exon II

text: Plasmid

text: pBluescriptIIKS+


text: ISV-CNRS

text: live

text: Generic

text: Physiological condition: greenhouse plants. tissue_type: Flowering tips

text: Arabidopsis thaliana

text: ecotype Landsberg erecta

text: Gif-SiliqueB

text: Phage

text: Lambda ZAPII oriented


text: Institut de Biologie Moleculair des Plantes du CNRS (IBMP)

text: live

text: Generic

text: tissue_type: Dry seeds

text: Arabidopsis thaliana

text: EcoRI

text: XhoI

text: ecotype columbia

text: Perp-dry-seedA

text: Phage

text: Lambda ZAPII oriented


text: Institute For Animal Health

text: live

text: Generic

text: Adult spleen, splenic cell.

text: Gallus gallus

text: COMPTON LINE7

text: Chicken spleen


text: Inst. of Toxicology

text: live

text: Generic

text: tissue_type = lung. cell_type = fibroblast. cell_line = IMR-90.

text: Homo sapiens

text: HL 1011


text: Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais

text: live

text: Generic

text: Total cellular RNA from male and female adult worms was extracted according to a modification (Puissant, C. and Houdebine, L. M. BioFeedback 8, 148-149, 1990) of the Guanidine Thiocyanate procedure (Chomczynski, P. and Sacchi, N. Anal. Biochem. 162, 156- 159, 1987). Poly (A)+ RNA was purified by oligo dT column and cDNA was synthesized as described previously (Adams, M. D. et al. Nature Genet. 4, 373-389, 1993). cDNA was ligated to a two fold molar excess of a NotI/HindIII digested plasmid DNA (lafmid BA vector, a phagemid derived from pEMBL, Adams, M. D. et al. Nature Genet. 4, 373-389, 1993)) and electroporated into E. coli strain DH10B (BRL). The library was amplified and further selected for clones containing long inserts (>500 bp) by purification of the plasmid DNA from a fragment of a 1% low-melting-point agarose gel, containing the smear of the library and electroporation into DH10B cells.

text: DH10B, JM109

text: Schistosoma mansoni

text: NotI

text: HindIII

text: NMRI

text: Schistosoma mansoni, adult worm, Gloria Franco

text: Phagemid

text: BA vector


text: live

text: Generic

text: ds-cDNA was prepared from oligo-dT selected mRNA by priming with a NotI oligo- dT oligomer and then adding the second strand to RNase-nicked DNA:RNA hybrid with DNA PolI. SalI adaptors were added to the ends, the ds-cDNAs were then digested with NotI and size-selected. These were directionally-cloned into the ZipLox phage vector, excised as plasmids, and then individually analyzed.

text: Zea mays

text: SalI

text: NotI

text: membrane-free polysomes from endosperm

text: Plasmid

text: ZipLox


text: Carnegie Institution

text: live

text: Generic

text: Poly(A)+ RNA was purified from developing stage III to stage V (Greenwood & Bewley, Can. J. Bot. 60:1751-1760, 1982) endosperm plus embryo of immature castor fruits. cDNA was synthesized and cloned into lambdaZAPII according to the instructions of the manufacturer (Stratagene): synthesis was primed from the poly(A) tail, and cloned directionally into XhoI (3') and EcoRI (5') sites. In few cases, sequence data indicated that this directionality was reversed. Partial cDNA clones predominate.

text: Ricinus communis

text: XhoI

text: Baker 296

text: lambdaZAPST

text: Phage

text: lambdaZAPII


text: Institute for Animal Health

text: live

text: Generic

text: embryo 17 days

characteristic: dev-stage

text: Gallus gallus

text: bursa (whole)

characteristic: tissue-type


text: live

text: Generic

text: oligo-dT selected mRNA was prepared from above-ground tissue from10 day-old etiolated seedlings. ds-cDNA was prepared by priming with a NotI oligo-dT oligomer and the second strand by RNase-nicking of the DNA:RNA hybrid with DNA PolI fill-in. SalI adaptors were added to the ends, the ds-cDNAs were then digested with NotI and size-selected. These were directionally-cloned intothe ZipLox phage vector, excised as plasmids, and then individually analyzed.

text: Zea mays

text: SalI

text: NotI

text: etiolated seedling

text: Plasmid

text: ZipLox


text: Yale University

text: live

text: Generic

text: A yeast genomic DNA library was prepared in the vector pHSS6, and subjected to transposon mutagenesis with mTn3. This mini-transposon carries lacZ sequences that lack an initiation codon; expression of lacZ is only provided by in frame fusion to yeast coding sequence. The yeast genomic DNA carrying the transposon was excised from pHSS6 and transplaced back onto the yeast chromosome. Yeast colonies expressing lacZ were screened for in a color assay. A plasmid containing the genomic DNA/lacZ fusion junction was recovered from each individual yeast colony that expressed lacZ activity. These recovered plasmids comprise 'The blue guys library'. The fusion junction was then sequenced to identify the expressed ORF upstream of the fusion.

text: E.coli

text: Saccharomyces cerevisiae

text: The blue guys library

text: Plasmid

text: pRECmTn


text: live

text: Generic

text: lambda unizap library from mail mouse testis

text: Mus musculus

text: Swiss

text: lambda unizap male testis

text: Phage

text: lambda unizap


text: live

text: Generic

text: dev_stage=adult; tissue_type=skeletal muscle; sequenced_mol=cDNA to mRNA library: of Charles Louis, Vet. Pathobiology, U. of Minn.

text: Sus scrofa

text: Pig adult skeletal muscle


text: live

text: Generic

text: cell_line=K562; cell_type=erythroleukemic; sequenced_mol=cDNA to mRNA

text: Homo sapiens

text: Human K562 erythroleukemic cells


text: University of Colorado Health Sciences Center

text: live

text: Generic

text: The normalized infant brain library, constructed by Bento Soares, Columbia University, was oligo-(dT) primed and directionally cloned into an M13-derived plasmid using total brain mRNA from a 72-day old human female afflicted with spinal muscular atrophy. The library was normalized as described elsewhere.

text: E. coli DH5-alpha

text: Homo sapiens

text: HindIII

text: NotI

text: Normalized infant brain, Bento Soares

text: Plasmid

text: BA, M13-derived


text: The Institute for Genomic Research

text: live

text: Generic

text: The infant brain library, constructed by Bento Soares, Columbia University, was oligo-(dT) primed and directionally cloned into an M13-derived plasmid using total brain mRNA from a 72-day old human female afflicted with spinal muscular atrophy.

text: E. coli DH5-alpha

text: Homo sapiens

text: HindIII

text: NotI

text: Infant brain, Bento Soares

text: Plasmid

text: BA, M13-derived


text: live

text: Generic

text: female 3 month post natal whole brain

text: Homo sapiens

text: Human whole brain


text: live

text: Generic

text: mRNA was purified from the eggs and embryos at every day of the gestation period. cDNA pools were equalized and cloned into the vector (Takahashi N and Ko MSH, unpublished; cf. Ko MSH, Nucleic Acids Res. 18: 5705-5711, 1990).

text: Mus musculus

text: NotI

text: SalI

text: Equalized mouse embryo cDNA library (EIV)

text: Plasmid

text: pBluescript SK(-)


text: University of Central Florida

text: live

text: Generic

text: PolyA+ RNA, from asynchronous blood stage parasites of the Dd2 isolate cultured in vitro, was reverse transcribed using an oligo dT-Xho I primer. Second strand was prepared using RNase H and DNA polymerase I. EcoR I adapters were ligated to the cDNA, and it was digested with Xho I. Prepared fragments were ligated into EcoR I + Xho I digested lambda ZAP II vector.

text: E. coli XL-1 blue

text: Plasmodium falciparum

text: EcoR I

text: Xho I

text: czapPFDd2.1, Debopam Chakrabarti

text: Phage

text: Lambda ZAP II


text: live

text: Generic

text: Cell line CGM

text: Homo sapiens

text: Human CGM


text: Michigan State University

text: live

text: Generic

text: 3 day old NT-1(By-2) cell library of Gao et al., Plant Mol. Biol. submitted.

text: Nicotiana tabacum

text: oligo dT primed cDNA library lzapII


text: Kyungpook National University

text: live

text: Generic

text: Poly(A)-mRNA was purified from the leaf of B.napus. cDNA library was constructed from the mRNAs by oligo(dT) priming and directionally cloned from the NotI site in the vector pT7T3D (Pharmacia) to the EcoRI site.

text: Brassica napus

text: NotI

text: EcoRI

text: cv. Naehan


text: University of Connecticut Health Center

text: live

text: Generic

text: E. crassus vegetative cell cDNA library. Vegetative stage. Macronuclear Randomly isolated.

text: Moneuplotes crassus

text: pEVR library


text: INSERM

text: live

text: Generic

text: mRNA was prepared from human testis of a 27 years old man. cDNA was prepared using a 15mer oligo dT anchored by two degenerated bases at its 3'end and containing a NotI site at its 5'end. The cDNA was cloned between EcoRV and NotI sites of PGEM 5zf(+). The 3' end is at the NotI site. The EcoRV site is lost during the cloning procedure. cDNA corresponding to abundant species were eliminated from this library.

text: Homo sapiens

text: EcoRV

text: Testis 1

text: Plasmid

text: PGEM 5zf(+)


text: INSERM

text: live

text: Generic

text: mRNA was prepared from human testis of a 27 years old man. cDNA was prepared using a 15mer oligo dT anchored by two degenerated bases at its 3'end and containing a NotI site at its 5'end. The cDNA was cloned between SalI and NotI sites of PGEM 5zf(+). The 3' end is at the NotI site. The Sal1 site is lost during the cloning procedure. cDNA corresponding to abundant species were eliminated from this library.

text: Homo sapiens

text: Testis 2

text: Plasmid

text: PGEM 5zf(+)


text: Children's Medical Research Institute

text: live

text: Generic

text: cDNA was reverse transcribed using oligo dT priming and cloned into the BamH1 site of the pUEX1 vector.

text: Gallus gallus

text: ED18 chick cerebellum, Peter Jeffrey

text: Plasmid


text: National Institute of Genetics

text: live

text: Generic

text: Female, adult, cell_line = HL60, cell_type = promyelocyte.

text: Homo sapiens

text: Human promyelocyte


text: National Institute of Genetics

text: live

text: Generic

text: Adult, primary culture of aortic endothelium

text: Homo sapiens

text: Human aortic endothelium


text: National Institute of Genetics

text: live

text: Generic

text: Adult male, tissue_type = colon mucosa

text: Homo sapiens

text: Human colon mucosa


text: live

text: Generic

text: Normalized infant brain cDNA library made by Dr. M. Soares (Columbia University), oligo-dT primed and directionally cloned between HindIII (5') and NotI (3') sites

text: Homo sapiens

text: HindIII

text: NotI

text: Infant brain, LLNL array of Dr. M. Soares 1NIB

text: lafmid BA


text: Lawrence Livermore National Laboratory

text: live

text: Generic

text: Directionally cloned, oligo dT-primed infant brain library made by M. Soares of Columbia University

text: Homo sapiens

text: HindIII

text: NotI

text: Infant brain library of Dr. M. Soares

text: lafmid BA


text: National University of Singapore

text: live

text: Generic

text: mRNA was purified from several pituitaries of the halibut. cDNA was directly cloned into the lambda ZAPII vector using the Stratagene's Lambda Uni-ZAP XR cDNA cloning system.

text: Hippoglossus hippoglossus

text: EcoRI

text: XhoI

text: halibut pituitary

text: Phage

text: Lambda Uni-ZAP XR


text: National University of Singapore

text: live

text: Generic

text: mRNA was purified from a liver of the rainbow trout. cDNA was directly cloned into the lambda ZAPII vector using the Stratagene's Lambda Uni-ZAP XR cDNA cloning system.

text: Oncorhynchus mykiss

text: EcoRI

text: XhoI

text: rainbow trout liver

text: Phage

text: Lambda Uni-ZAP XR


text: National University of Singapore

text: live

text: Generic

text: mRNA was purified from several pituitaries of the winter flounder. cDNA was directly cloned into the lambda ZAPII vector using the Stratagene Lambda Uni-ZAP XR cDNA cloning system.

text: Pseudopleuronectes americanus

text: EcoRI

text: XhoI

text: winter flounder pituitary

text: Phage

text: Lambda Uni-ZAP XR


text: National University of Singapore

text: live

text: Generic

text: mRNA was purified from several pituitaries of the chinook salmon. cDNA was directly cloned into the lambda ZAPII vector using the Stratagene's Lambda Uni-ZAP XR cDNA cloning system.

text: Cyclopterus lumpus

text: EcoRI

text: XhoI

text: chinook salmon pituitary

text: Phage

text: Lambda Uni-ZAP XR


text: National University of Singapore

text: live

text: Generic

text: mRNA was purified from several pituitaries of the lumpfish. cDNA was directly cloned into the lambda ZAPII vector using the Stratagene's Lambda Uni-ZAP XR cDNA cloning system.

text: Cyclopterus lumpus

text: EcoRI

text: XhoI

text: lumpfish pituitary

text: Phage

text: Lambda Uni-ZAP XR


text: live

text: Generic

text: Devel_stage = flower bud

text: Brassica rapa

text: pekinensis

text: Mustard flower buds


text: laboratoire de Biologie Moleculaire

text: live

text: Generic

text: late log phase

text: Arabidopsis thaliana

text: ecotype Columbia

text: cell suspension culture

text: Phage

text: Lambda ZAPII


text: Children's Memorial Research Center

text: live

text: Generic

text: cDNA from polyA+ RNA from human brain. 3-month old infant.

text: E. coli

text: Homo sapiens

text: Not I

text: EcoR I

text: Plasmid

text: Lafmid BK


text: Children's Memorial Research Center

text: live

text: Generic

text: E. coli

text: Homo sapiens

text: Not I

text: Hind III

text: Plasmid

text: Lafmid BA


text: Children's Memorial Research Center

text: live

text: Generic

text: Attempted normalization of a human fetal brain cDNA library from polyA+ RNA.

text: E. coli

text: Homo sapiens

text: Not I

text: Hind III

text: Plasmid

text: Lafmid A


text: Children's Memorial Research Center

text: live

text: Generic

text: cDNA from polyA+ RNA from human fetal liver + spleen. 20 wee ks post-conception.

text: E. coli

text: Homo sapiens

text: Not I

text: EcoR I

text: Plasmid

text: Lafmid K


text: Children's Memorial Research Center

text: live

text: Generic

text: Size-selected cDNA from polyA+ RNA from human brain. 3-month old infant. This library is putatively enriched for the abu ndant clones from library b4HB3MA.

text: E. coli

text: Homo sapiens

text: Not I

text: Hind III

text: Plasmid

text: Lafmid BA


text: Children's Memorial Research Center

text: live

text: Generic

text: Size-selected cDNA from polyA+ RNA from human brain. 3-month old infant. This library is the result of an attempted no rmalization of library b4HB3MA.

text: E. coli

text: Homo sapiens

text: Not I

text: Hind III

text: Plasmid

text: Lafmid BA


text: live

text: Generic

text: E. coli

text: Homo sapiens

text: Not I

text: Hind III

text: Plasmid

text: Lafmid BA


text: Children's Memorial Research Center

text: live

text: Generic

text: Size-selected cDNA from polyA+ RNA from human brain. 3-month old infant.

text: E. coli

text: Homo sapiens

text: Not I

text: EcoR I

text: Plasmid

text: Lafmid BK


text: Children's Memorial Research Center

text: live

text: Generic

text: Size-selected cDNA from polyA+ RNA from human fetal liver + spleen. 20 weeks post-conception.

text: E. coli

text: Homo sapiens

text: Not I

text: EcoR I

text: Plasmid

text: Lafmid K


text: Children's Memorial Research Center

text: live

text: Generic

text: cDNA from polyA+ RNA from human brain. 3-month old infant. T his library is the result of attempted normalization of libr ary b4HB3MA.

text: E. coli

text: Homo sapiens

text: Not I

text: Hind III

text: Plasmid

text: Lafmid BA


text: Children's Memorial Research Center

text: live

text: Generic

text: Size-selected cDNA from polyA+ RNA from human brain. 3-month old infant. This library is the result of an attempted nor malization of library b4HB3MA.

text: E. coli

text: Homo sapiens

text: Not I

text: Hind III

text: Plasmid

text: Lafmid BA


text: live

text: Generic

text: cDNA from polyA+ RNA from human brain. 3-month old infant. T his library is the result of attempted normalization of libr ary 4HB3MA.

text: E. coli

text: Homo sapiens

text: Not I

text: Hind III

text: Plasmid

text: Lafmid BA


text: Children's Memorial Research Center

text: live

text: Generic

text: cDNA libray from polyA+ RNA from human brain: white matter f rom an adult with multiple sclerosis

text: E. coli

text: Homo sapiens

text: Not I

text: EcoR I

text: HMSWMYK

text: Plasmid

text: Lafmid BK


text: Children's Memorial Research Center

text: live

text: Generic

text: cDNA libray from polyA+ RNA from human brain: white matter f rom a 42 year old patient with multiple sclerosis

text: E. coli

text: Homo sapiens

text: Not I

text: Hind III

text: Plasmid

text: Lafmid BA


text: Children's Memorial Research Center

text: live

text: Generic

text: Attempted normalization of library b4HB3MA. Size-selected cD NA from polyA+ RNA from human brain. 3-month old infant.

text: E. coli

text: Homo sapiens

text: Not I

text: Hind III

text: Plasmid

text: Lafmid BA


text: Children's Memorial Research Center

text: live

text: Generic

text: Attempted normalization of 2HFBA. cDNA from polyA+ RNA from human fetal brain

text: E. coli

text: Homo sapiens

text: Not I

text: Hind III

text: Plasmid

text: Lafmid A


text: Children's Memorial Research Center

text: live

text: Generic

text: cDNA from polyA+ RNA from human brain. 3-month old infant. T his library is the result of attempted normalization of libr ary HB3MK.

text: E. coli

text: Homo sapiens

text: Not I

text: EcoR I

text: Plasmid

text: Lafmid BK


text: live

text: Generic

text: Size-selected cDNA from polyA+ RNA from human brain. 3-month old infant

text: E. coli

text: Homo sapiens

text: Not I

text: EcoR I

text: Plasmid

text: Lafmid BK


text: National Institute of Genetics

text: live

text: Generic

text: varied

text: Caenorhabditis elegans

text: hermaphrodite, male

characteristic: sex

text: CB1489 him-8(e1489)

text: whole animal

text: Yuji Kohara unpublished cDNA


text: Children's Memorial Research Center

text: live

text: Generic

text: Size-selected cDNA from polyA+ RNA from human brain. 3-month old infant. This library is putatively enriched for the abundant clones from library b4HB3MA.

text: E. coli

text: Homo sapiens

text: Not I

text: Hind III

text: Plasmid

text: Lafmid BA


text: live

text: Generic

text: tissue_type = brain striatum

text: Homo sapiens

text: Human brain striatum


text: Institute of Biomedical and Life Sciences, Division of Molecular Genetics

text: live

text: Generic

text: tissue=ventral midbrain; dev_stage=8 week old mice

text: Mus musculus

text: outbred

text: MVM (mouse ventral midbrain)


text: live

text: Generic

text: tissue=front of brain; dev_stage=8 week old mice

text: Mus musculus

text: outbred

text: MF (mouse front of brain)


text: live

text: Generic

text: tissue_type=hippocampus; dev_stage=adult rat

text: Rattus norvegicus

text: Wistar

text: RH (rat hippocampus)


text: live

text: Generic

text: tissue_type=hypothalamus; dev_stage=adult rat

text: Rattus norvegicus

text: Wistar

text: RHy (rat hypothalamus)


text: live

text: Generic

text: mRNA was purified from a colorectal tumour of an adult male. cDNA was constructed and cloned into the pT7T3D phagemid following the manufacturer intructions (Pharmacia).

text: E. coli NM522

text: Homo sapiens

text: Eco RI

text: Not I

text: Human colorectal cancer


text: Institute For Animal Health

text: live

text: Generic

text: Female adult chicken spleen, T lymphocyte cells.

text: Gallus gallus

text: COMPTON LINE7


text: Howard Hughes Medical Institute

text: live

text: Generic

text: Tissue_type = lymphocyte; cell_line = 204-1-8; cell_type = pre-B cell

text: Mus musculus

text: caffeine-1-8 cDNA RDA third difference product


text: live

text: Generic

text: Whole brain, from 26 week old male fetus

text: Homo sapiens

text: ClonTech HL 1065a


text: Kyoto Prefectural University of Medicine

text: live

text: Generic

text: keratinocyte

characteristic: cell type

text: primary culture

text: Homo sapiens

text: epidermis

text: Human epidermal keratinocyte


text: Faculty Veterinary Medicine, University of Montreal

text: live

text: Generic

text: cDNA derived from bovine adult testicles amplified by PCR and cloned blunt into pGEM(t) vector

text: Bos taurus

text: EcoRI

text: EcoRI

text: Holstein

text: adult bovine testicles

text: Plasmid

text: pGEM(t), PROMEGA

characteristic: vector


characteristic: External Id

text: live

text: Generic

text: Bos taurus

text: EcoRI

text: EcoRI

text: Holstein

text: 6 month bovine thymus

text: Plasmid

text: pGEM(t), PROMEGA


characteristic: INSDC center name

text: live

text: Generic

text: cDNA derived from bovine adult corpus luteum amplified by PCR and cloned blunt into pGEM(t) vector

text: Bos taurus

text: EcoRI

text: EcoRI

text: Holstein

text: adult bovine corpus luteum

text: Plasmid

text: pGEM(t), PROMEGA


characteristic: INSDC first public

text: live

text: Generic

text: cDNA derived from bovine adult cotyledon amplified by PCR and cloned blunt into pGEM(t) vector

text: Bos taurus

text: EcoRI

text: EcoRI

text: Holstein

text: adult bovine cotyledon

text: Plasmid

text: pGEM(t), PROMEGA


characteristic: INSDC last update

text: live

text: Generic

text: cDNA derived from bovine adult pituitary amplified by PCR and cloned blunt into pGEM(t) vector

text: Bos taurus

text: EcoRI

text: EcoRI

text: Holstein

text: adult bovine pituitary

text: Plasmid

text: pGEM(t), PROMEGA


text: live

characteristic: INSDC status

text: Generic

text: Arrayed cDNAs and cosmid libraries from human placental tissue

text: Homo sapiens

text: Human placenta


text: Baylor College of Medicine

text: live

text: Generic

characteristic: NCBI submission model

text: oligo dT-primed cDNA prepared from polyA RNA from BL29 cell line. Size fractionated from 300bp to 3000bp. cDNA cloned into lambda Zap using EcoRI linkers in random orientation

text: Homo sapiens

text: EcoRI

text: EcoRI

text: BL29 Burkitt's lymphoma, Pascalis Sideras

text: Phage

text: lambda ZAP 2


text: Hopital Necker-Enfants Malades

text: live

text: Generic

characteristic: NCBI submission package

text: Fetal kidney cDNA (28 weeks) was subtracted by an excess of adult kidney mRNA, ligated to 20mers BamHI adaptors and cloned into pUEX1.

text: Escherichia coli MC1061

text: Homo sapiens

text: BamHI

text: BamHI

text: NEM subtracted human fetal kidney cDNA

text: Plasmid


text: Kyungpook National University

text: live

text: Generic

text: Poly(A)-mRNA from the 2-year old male fetal thymus, oligo(dT) priming, EcoRI cloning in the vector pBluescipt (Stratagene).

characteristic: description

text: Homo sapiens

text: EcoRI

text: EcoRI

text: pBluescript SK(-)


text: Washington University School of Medicine

text: live

text: Generic

text: mRNA was purified from non-diabetic human islets

text: Homo sapiens

characteristic: organism

text: Eco R1

text: Eco R1

text: Human islet

text: Phage

text: Lambda gt11


text: Genethon Centre de recherche sur le Genome Humain

text: live

text: Generic

text: sex=Female; dev_stage=3 months old; isolate=muscular atrophy patient; tissue_type=total brain; total mRNA was oligo-(dT) primed and directionally cloned 5' -> 3' into the HindIII -> NotI sites of the lafmid BA vector. Clone library from B.Soares, Psychiatry Dept. Columbia University, USA. Normalization_method: Bento Soares, P.N.A.S in press

text: 3 months old

text: brain

characteristic: organ

text: Homo sapiens

text: HindIII

text: NotI

text: Female

text: total brain

text: normalized infant brain cDNA

text: lafmid BA


text: University of Washington

text: live

text: Generic

text: mRNA was purified from human CD34+ cells. cDNA was synthesized using Stratagene's ZAP-cDNA synthesis kit(Cat#200400). cDNA was directionally cloned into EcoRI and XhoI sites in vector Lambda ZAPII

text: Homo sapiens

text: EcoRI

text: XhoI

characteristic: title

text: Phage

text: Lambda ZAPII


text: Wayne State University

text: live

text: Generic

text: mRNA was purified from the mouse Ltk- fibroblastoid cultured cells. Double-stranded cDNAs were synthesized from the mRNA using an oligo(dT)-NotI primer. After shearing to 200-400 bp, a synthetic linker-primer, which has one blunt and one sticky end and an internal EcoRI site, was ligated to the cDNAs. The cDNAs were amplified by the polymerase chain reaction (PCR) using the ligated linker-primer sequence. The cDNAs were digested with EcoRI and NotI, and inserted into a plasmid vector.

text: Mus musculus

text: NotI

text: EcoRI

characteristic: strain

text: Unequalized cDNA library from Ltk- cultured cells

text: Plasmid

text: pBluescript SK(-)


text: Wayne State University

text: live

text: Generic

text: mRNA was purified from the mouse Ltk- fibroblastoid cultured cells. Double-stranded cDNAs were synthesized from the mRNA using an oligo(dT)-NotI primer. After shearing to 200-400 bp, a synthetic linker-primer, which has one blunt and one sticky end and an internal EcoRI site, was ligated to the cDNAs. The cDNAs were amplified by the polymerase chain reaction (PCR) using the ligated linker-primer sequence. After denaturation and reassociation of the ds-cDNAs, and isolation of single- stranded cDNAs by hydroxyapatite chromatography, the single- stranded cDNAs were again amplified by PCR. The cDNAs were digested with EcoRI and NotI, and inserted into a plasmid vector.

text: Mus musculus

text: NotI

text: EcoRI

text: Equalized cDNA library from Ltk- cultured cells

text: Plasmid

text: pBluescript SK(-)


text: Faculty Veterinary Medicine, University of Montreal

text: live

text: Generic

text: cDNA derived from 6 month bovine thyroid amplified by PCR and cloned blunt into pGEM(t) vector

text: Bos taurus

text: EcoRI

text: EcoRI

text: Holstein

text: 6 month bovine thyroid

text: Plasmid

text: pGEM(t), PROMEGA


text: International Livestock Research Institute

text: live

text: Generic

text: The mRNA was purified from a cloned population of bloodstream trypanosomes reexpressing the MVAT4 metacyclic variant surface glycoprotein (VSG). A unidirectional oligo dT-primed EcoRI/XhoI cDNA library was constructed in lambda ZAP II (Stratagene).

text: Trypanosoma brucei rhodesiense

text: EcorI

text: XhoI

text: MVAT4 bloodstream form of serodeme WRATat1.1

text: Phage

text: Lambda ZAP II (Stratagene)


text: University of Colorado Health Sciences Center

text: live

text: Generic

text: developmental-stage=Embryonic Day 17 Post-fertilization; tissue-type=Aorta; cell-type=Vascular smooth muscle cell; sex=Male.

text: Rattus norvegicus

text: Sprague-Dawley

text: Lambda Zap Express library of P. E. Schwartz (9/93)


text: University of Arizona

text: live

text: Generic

text: Cell line = Wehi7.2

text: Mus musculus

text: Mouse Wehi7.2


text: CNRS

text: live

text: Generic

text: dev-stage=adult; tissue-type=adipose tissue; lab_host=Bacteriophage lambda; first strand priming with random and poly-d(T) oligonucleotides.

text: Homo sapiens

text: EcoRI

text: Caucasian

text: Clontech adult human fat cell library HL1108A

text: Phage

text: lambda gt10


text: NCHGR/NIH

text: live

text: Generic

text: Homo sapiens

text: Human Genomic

text: Plasmid

text: pAMP


text: Institut Municipal Investigacio Medica (IMIM)

text: live

text: Generic

text: Poly A+ RNA was extracted from the colon adenocarcinoma cell line HT29M6. cDNA synthesis was primed with oligo-dT and the products were directionally cloned in the lambda-ZAPII vector.

text: E. coli XL1Blue

characteristic: lab-host

text: Homo sapiens

text: Eco RI

text: Xho I

text: Phage

text: Lambda ZAPII


text: CNRS

text: live

text: Generic

text: White adipose tissue, sub cutaneous, adult, female. Purification of polyA mRNA, first strand priming with random oligonucleotides. Ligation of adaptors EcoRI/NotI, cloning in EcoRI site of lambda gt11.

text: Homo sapiens

text: EcoRI

text: caucasian

text: lambda gt11


text: University of Texas Southwestern Medical Center at Dallas

text: live

text: Generic

text: Direct selection of expressed sequences within the BRCA1 region

text: Homo sapiens

text: Human chromosome 17


text: Jawaharlal Nehru University

text: live

text: Generic

text: trophozoite

text: trophozoite

characteristic: dev-stage

text: Entamoeba histolytica

text: Entamoeba cDNA to mRNA


text: CNRS

text: live

text: Generic

text: Brown adipose tissue, perirenal, children Purification of polyA mRNA, first strand priming with random oligonucleotides Ligation of adaptors EcoRI/NotI, cloning in EcoRI site of lambda gt10.

text: Homo sapiens

text: EcoRI

text: lambda gt10


text: Department of Human Genetics

text: live

text: Generic

text: Homo sapiens

text: Human, Meese.E.


text: Medizinische Klinik 1

text: live

text: Generic

text: Whole insect, dev_stage=embryonal 0 to 4h. Library of Brown & Kafatos, JMB 203:425(1988)

text: Drosophila melanogaster

text: dp cn bw

text: Fruit fly embryo


text: University of Chicago

text: live

text: Generic

text: mRNA was purified from 14.7 hybrid MN cells and used to construct a size- selected unidirectional cDNA library in Uni-Zap XR vector.

text: Mus musculus

text: EcoR I

text: Xho I

text: Phage

text: Uni-Zap XR


text: live

text: Generic

text: Homo sapiens

text: adipose

characteristic: tissue-type

text: Human Adipose tissue


text: live

text: Generic

text: adrenal gland

text: Homo sapiens

text: Human Adrenal gland


text: live

text: Generic

text: Homo sapiens

text: bone

text: Human Bone


text: live

text: Generic

text: brain

text: Homo sapiens

text: Human Brain


text: live

text: Generic

text: breast

text: Homo sapiens

text: Human Breast


text: live

text: Generic

text: colon

text: Homo sapiens

text: Human Colon


text: live

text: Generic

text: Homo sapiens

text: embryo

text: Human Embryo


text: live

text: Generic

text: Homo sapiens

text: endothelial

text: Human Endothelial cells


text: live

text: Generic

text: Homo sapiens

text: epididymis

text: Human Epididymis


text: live

text: Generic

text: esophagus

text: Homo sapiens

text: Human Esophagus


text: live

text: Generic

text: eye

text: Homo sapiens

text: Human Eye


text: live

text: Generic

text: gallbladder

text: Homo sapiens

text: Human Gall bladder


text: live

text: Generic

text: greater omentum

text: Homo sapiens

text: Human Greater omentum


text: live

text: Generic

text: heart

text: Homo sapiens

text: Human Heart


text: live

text: Generic

text: kidney

text: Homo sapiens

text: Human Kidney


text: live

text: Generic

text: liver

text: Homo sapiens

text: Human Liver


text: live

text: Generic

text: lung

text: Homo sapiens

text: Human Lung


text: live

text: Generic

text: Homo sapiens

text: lymphoid

text: Human Lymphoid tissue


text: live

text: Generic

text: ovary

text: Homo sapiens

text: Human Ovary


text: live

text: Generic

text: pancreas

text: Homo sapiens

text: Human Pancreas


text: live

text: Generic

text: parathyroid gland

text: Homo sapiens

text: Human Parathyroid gland


text: live

text: Generic

text: placenta

text: Homo sapiens

text: Human Placenta


text: live

text: Generic

text: blood

text: Homo sapiens

text: platelet

text: Human Platelet


text: live

text: Generic

text: prostate gland

text: Homo sapiens

text: Human Prostate gland


text: live

text: Generic

text: salivary gland

text: Homo sapiens

text: Human Salivary gland


text: live

text: Generic

text: Homo sapiens

text: skeletal muscle

text: Human Skeletal muscle


text: live

text: Generic

text: skin

text: Homo sapiens

text: Human Skin


text: live

text: Generic

text: small intestine

text: Homo sapiens

text: Human Small intestine


text: live

text: Generic

text: spleen

text: Homo sapiens

text: Human Spleen


text: live

text: Generic

text: synovial membrane

text: Homo sapiens

text: Human Synovial membrane


text: live

text: Generic

text: testis

text: Homo sapiens

text: Human Testis


text: live

text: Generic

text: thymus gland

text: Homo sapiens

text: Human Thymus gland


text: live

text: Generic

text: thyroid gland

text: Homo sapiens

text: Human Thyroid gland


text: live

text: Generic

text: uterus

text: Homo sapiens

text: Human Uterus


text: live

text: Generic

text: blood

text: Homo sapiens

text: white blood cells

text: Human White blood cells


text: Research Institute for Microbial Diseases, Osaka University

text: live

text: Generic

text: Mus musculus

text: mouse embryonal carcinoma cell line F9


text: live

text: Generic

text: Saccharomyces cerevisiae

text: EcoRI

text: XhoI

text: S. cerevisiae strain X2180-1A

text: pBluescript SK-


text: live

text: Generic

text: brain

text: Homo sapiens

text: HindII

text: NotI

text: Infant Brain

text: lafmid BA


text: The Institute for Genomic Research

text: live

text: Generic

text: brain

text: Homo sapiens

text: EcoRI

text: EcoRI

text: Fetal brain

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: brain

text: Homo sapiens

text: EcoRI

text: EcoRI

text: hippocampus

text: Hippocampus

text: pBluescript SK-


text: live

text: Generic

text: isolate=Eucalyptus globulus bicostata symbiont tissue_type=ectomycorrhiza

text: Pisolithus tinctorius


text: The Chinese University of Hong Kong

text: live

text: Generic

text: E. coli Y1090

text: Homo sapiens

text: EcoRI

text: EcoRI

text: Adult heart, Clontech

text: Phage

text: Lambda gt11


text: Shemyakin Institute of Bioorganic Chemistry

text: live

text: Generic

text: The library was constructed as described in [Obradovic, D., Borodin, A.M., Kopantzev, E.P., Wagner, L.L., Volik, S.V., Ermolaeva, O.D., Lebedev, Y.B., Monastyrskaya, G.S., Sverdlov, E.D. (1993) Bioorganicheskaya khimia, 20, 919-930]. This protocol is based on nested primer strategy using Alu- specific primers (ALN3 and TC-65) that direct the hncDNA synthesis outward of Alu repeats.

text: Homo sapiens

text: Outward Alu-primed hncDNA library

text: Plasmid


text: live

text: Generic

text: The library was constructed as described in [Obradovic, D., Borodin, A.M., Kopantzev, E.P., Wagner, L.L., Volik, S.V., Ermolaeva, O.D., Lebedev, Y.B., Monastyrskaya, G.S., Sverdlov, E.D. (1993) Bioorganicheskaya khimia, 20, 919-930]. This protocol is based on nested primer strategy using Alu- specific primers (ALN1 and 517) that direct the hncDNA synthesis inward of Alu repeats.

text: Homo sapiens

text: Inward Alu-primed hncDNA library

text: Plasmid


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. normal lung. Average insert size: 1.0 kb; Uni-ZAP XR Vector; ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'

text: 72 years

text: SOLR cells (kanamycin resistant)

text: lung

text: Homo sapiens

text: EcoRI

text: XhoI

text: male

characteristic: sex

text: Stratagene lung (#937210)

text: phagemid

text: pBluescript SK-


text: Washington University School of Medicine

text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Caucasian. Average insert size: 1.2 kb; Uni-ZAP XR Vector; ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'

text: SOLR cells (kanamycin resistant)

text: placenta

text: Homo sapiens

text: EcoRI

text: XhoI

text: male

text: Stratagene placenta (#937225)

text: phagemid

text: pBluescript SK-

characteristic: vector


characteristic: External Id

text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Pooled spleens. Average insert size: 1.0 kb; Uni-ZAP XR Vector; ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'

text: fetal

text: SOLR cells (kanamycin resistant)

text: spleen

text: Homo sapiens

text: EcoRI

text: XhoI

text: fetal spleen

text: Stratagene fetal spleen (#937205)

text: phagemid

text: pBluescript SK-


characteristic: INSDC center name

text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Hepatectomy from normal male caucasian. Average insert size: 1.1 kb; Uni-ZAP XR Vector; ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'

text: 49 years old

text: SOLR cells (kanamycin resistant)

text: liver

text: Homo sapiens

text: EcoRI

text: XhoI

text: male

text: Stratagene liver (#937224)

text: phagemid

text: pBluescript SK


characteristic: INSDC first public

text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Total ovary tissue, normal, caucasian. Average insert size: 0.8 kb; Uni-ZAP XR Vector; ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'

text: 49 year old

text: SOLR cells (kanamycin resistant)

text: ovary

text: Homo sapiens

text: EcoRI

text: XhoI

text: female

text: Stratagene ovary (#937217)

text: phagemid

text: Bluescript SK


text: Karolinska Institutet

characteristic: INSDC last update

text: live

text: Generic

text: Testis from adult mouse

text: Mus musculus

text: CBA

text: Mouse testis T-ZAP


text: The Samuel Roberts Noble Foundation, Inc.

text: live

characteristic: INSDC status

text: Generic

text: mRNA was purified from soybean etiolated hypocotyls. cDNA was constructed using an XhoI/oligo (dT)-linker/primer and cloned unidirectionally into the EcoRI and XhoI sites of the lambda phage Uni-ZAP (Stratagene). The library was screened using an anti-plasma membrane serus preabsorbed with an E. coli/phage lysate.

text: Glycine max

text: EcoRI

text: XhoI

text: cv Williams 82

text: Soybean, M Bhattacharyya

text: Phage

text: Uni-ZAP


text: University of Notre Dame

text: live

text: Generic

characteristic: NCBI submission model

text: cDNA was directionally synthesized from the XbaI site in the vector to the EcoRI site.

text: unbloodfed adult

text: Aedes aegypti

text: EcoRI

text: XbaI

text: female

text: Liverpool

text: whole body

text: LIV female


text: University of Tokyo

text: live

text: Generic

characteristic: NCBI submission package

text: Homo sapiens

text: Human fetal lung


text: Boston University Medical Center Hospital

text: live

text: Generic

text: Gastrular stage embryonic tissue

characteristic: description

text: Danio rerio

text: Zebra fish embryo


text: National Institute of Genetics

text: live

text: Generic

text: Adult human lung, 3' directed MboI

text: adult

text: lung

text: Homo sapiens

characteristic: organism

text: Human adult lung 3' directed MboI cDNA


text: live

text: Generic

text: Toxoplasma RH strain tachyzoites were grown in human foreskin fibroblast cultures in vitro. The library was constructed by K.L. Wan, Cambridge University. cDNAs were synthesized from polyA RNAs by oligo d(T) priming and directionally cloned into the EcoRI to XhoI sites of the Lambda ZapII vector using the ZAP-cDNA synthesis kit (Stratagene). WARNING: the library contains a small percentage of cDNAs derived from the human host cells.

text: XL1-Blue MRF'

text: Toxoplasma gondii

text: EcoRI

text: XhoI

text: RH

text: TgRH Tachyzoite cDNA

text: Lambda ZAP


text: Department of Forest Science

text: live

text: Generic

text: mRNA was purified from Atriplex canescens exposed to 200 ppb of ozone for 7 days. cDNA was constructed and cloned into Lamda ZAP phagemid. cDNA was directionally inserted between EcoR1 and Xho 1 site.

text: Atriplex canescens

text: EcoRI

text: XhoI

text: OICL, FMB, TAMU

text: Phage

text: Lamda ZAP


text: live

text: Generic

text: 1st strand cDNA was primed with a Pac I - oligo(dT) primer [5' AACTGGAAGAATTAATTAAAGATCTTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Pac I and cloned into the Pac I and Eco RI sites of the modified pT7T3 vector. Library went through one round of normalization. Library constructed by Bento Soares and M.Fatima Bonaldo.

text: 20 week-post conception fetus

text: DH10B (ampicillin resistant)

text: Liver and Spleen

characteristic: organ

text: Homo sapiens

text: Pac I

text: Eco RI

text: male

text: Soares fetal liver spleen 1NFLS

text: phagemid

text: pT7T3D (Pharmacia) with a modified polylinker


text: live

text: Generic

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' AACTGGAAGAATTCGCGGCCGCAGGAATTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Hind III adaptors (Pharmacia), digested with Not I and directionally cloned into the Not I and Hind III sites of the Lafmid BA vector. Library went through one round of normalization. Library constructed by Bento Soares and M.Fatima Bonaldo.

text: 73 days post natal

text: DH10B (ampicillin resistant)

text: whole brain

text: Homo sapiens

text: Not I

text: Hind III

text: female

text: Soares infant brain 1NIB

characteristic: title

text: phagemid

text: Lafmid BA


text: Universidade Federal do Para

text: live

text: Generic

text: Mid or late log promastigotes.Full length : splice leader oligo used for second strand synthesis, directional cloning NotI at 5' end, XhoI at 3' end.

text: XL1-Blue MRF'

text: Leishmania major

text: NotI

text: XhoI

characteristic: strain

text: Lambda UNIZAP


text: live

text: Generic

text: ds-cDNA was prepared from oligo-dT selected mRNA by priming with a XhoI oligo- dT oligomer and then adding the second strand to RNase-nicked DNA:RNA hybrid with DNA PolI. EcoRI adaptors were added to the ends, the ds-cDNAs were then digested with EcoRI- XhoI and size-selected. These were directionally-cloned into the ZAP phage vector, excised as plasmids, and then individually analyzed.

text: SOLR

characteristic: lab-host

text: Zea mays

text: EcoRI

text: XhoI

text: vegetative meristem

text: Plasmid

text: Zap


text: Hoffmann-La Roche Inc.

text: live

text: Generic

text: primary culture of dermal microvascular endothelial cell, from endothelial cells of foreskin

text: Homo sapiens

text: HDMEC cDNA library


text: Kellogg Eye Center, University of Michigan

text: live

text: Generic

text: Swaroop et al. (1991) Nucleic Acids Res. 19: 1954.

text: Homo sapiens

text: Subtracted human retina


text: Concordia University

text: live

text: Generic

text: cDNA was synthesized with ZAP kit (Stratagene) using poly(A)+ RNA isolated from Aspergillus niger grown in both complete and minimal media. Synthesis was primed with oligo(dT) primer/XhoI-linker. EcoRI adaptors were later ligated to polished ends. EcoRI-XhoI-digested cDNA was ligated with EcoRI-XhoI-digested pYES2 (Invitrogen Corp). This vector permits expression of our library in yeast.

text: E. coli

text: Aspergillus niger

text: Xho-I

text: EcoRI

text: Aspergillus niger, pYES2 (XhoI-EcoRI)

text: plasmid


text: Washington University School of Medicine

text: live

text: Generic

text: Stage:mixed, Sex:hermaphrodite. Library construction: First strand oligo(dT) primed. Second strand was as per Gubler/Hoffman. Ligated to EcoRI adaptors. Library is non-directional. Library is non-normalized. Library constructed by P.E. Kuwabara. Additional details on construction of the library are described in P.E. Kuwabara and S. Shah, NAR 22: 4414 - 4418 (1994). Adaptor sequence: GAATTC CGTTGCTGTCG

text: Caenorhabditis briggsae

text: EcoRI

text: EcoRI

text: G16 Gujarat

text: Kuwabara Mixed stage C. briggsae

text: Phage

text: Lambda gt10


text: Max Planck Institut fuer Molekulare Genetik

text: live

text: Generic

text: Homo sapiens

text: Selected chromosome 21 cDNA library


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' AACTGGAAGAATTCGCGGCCGCAGGAATTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library went through one round of normalization. Library constructed by Bento Soares and M.Fatima Bonaldo.

text: placenta obtained at birth (full term)

characteristic: dev-stage

text: DH10B (ampicillin resistant)

text: placenta

text: Homo sapiens

text: Not I

text: Eco RI

text: Female

text: Soares placenta Nb2HP

text: phagemid


text: IUPUI

text: live

text: Generic

text: Newborn (P1) mice.

text: Mus musculus

text: C57BL/6J x CBA

text: Mouse cerebellum


text: Institut fuer Genbiologische Forschung

text: live

text: Generic

text: mRNA was isolated from epidermal fragments, in which the predominant living cells are guard cells. The cDNA library was constructed as per manufacturer's instructions (Stratagene, CA.). cDNA are directionally cloned, with the 5' end proximal to the EcoRI site and the 3' end proximal to the XhoI site. There is an additional NotI site downstream of the EcoRI site, because EcoRI/NotI linker adaptors (Pharmacia) were used in the construction of the library, instead of simply EcoRI adaptors.

text: Solanum tuberosum

text: EcoRI

text: XhoI

text: Desiree

text: Epidermal Fragment cDNA Library

text: Phage

text: Lambda ZapII


text: Pohang Institute of Science & Technology

text: live

text: Generic

text: The cDNA library made by oligo-dT primed and directionally cloned between 5'ExoR I-XhoI3' sites.

text: XL1-blue MRF`

text: Homo sapiens

text: EcoRI

text: XhoI

text: 22 week old human fetal liver cDNA library

text: pBluescriptII SK(-)


text: DKFZ (German Cancer Research Center)

text: live

text: Generic

text: Hydra vulgaris

text: 3'RACE product of Hydra vulgaris mRNA

text: pBluescript II SK+


text: Harvard Medical School

text: live

text: Generic

text: mRNA was purified from human fetal hearts (10-12 weeks). cDNA was constructed using a NotI-Oligo dT adaptor-primer. SalI adaptors were ligated, followed by digestion with NotI, for direction cloning into predigested lambda gt22. Method is described in J. Mol. Cell. Cardiol. (1994) 26, 1329-1333)

text: E. coli Y1090

text: Homo sapiens

text: NotI

text: SalI

text: Fetal heart

text: Phage

text: Lambda gt22


text: Yale University Medical School

text: live

text: Generic

text: A subtractive cDNA library was developed by hybridizing antisense, single-stranded phagemid DNA (ssDNA) (as pBluescript SK-) from the ocular ciliary body cDNA library (target) of a 34-year-old female donor in lambda-Uni-ZAP XR with biotinylated sense RNA of an ocular cell line cDNA library (driver) in the same vector.

text: Homo sapiens

text: EcoRI

text: XhoI

text: Subtractive cDNA library ocular ciliary body

text: pBluescript II SK


text: live

text: Generic

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCCCTTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of a modified pT7T3 vector (Pharmacia). Library went through one round of normalization to a Cot = 230. Library constructed by Bento Soares and M.Fatima Bonaldo.

text: adult

text: DH10B (ampicillin resistant)

text: breast

text: Homo sapiens

text: Not I

text: Eco RI

text: Female

text: Soares breast 2NbHBst

text: phagemid

text: pT7T3D (Pharmacia) with a modified polylinker


text: University of Edinburgh / School of Hygiene and Public Health, The Johns Hopkins University

text: live

text: Generic

text: Full length cDNA was prepared by RT-PCR from mRNA from vector-stage infective L3 larvae of the human filarial nematode parasite Brugia malayi using nematode spliced leader (SL, 5' end) and oligo-d(T) (3' end) primers. The library had an unamplified titre of 1.6 x 10E6 per ml and 94% of clones have inserts (mean length ~700 bp).

text: XL1-Blue MRF-

text: Brugia malayi

text: XbaI

text: EcoRI

text: Brugia malayi infective L3 JHU93SL-BmL3

text: Phage

text: lambda ZapII


text: NIH/NIDR

text: live

text: Generic

text: The apical portion and the entire pulp of the maxillary and mandibular incisors from 3-4 week old rats were excised. A cDNA library was constructed in the lZAP II vector (Stratagene) by use of poly A+ RNA from these tissues. The phage cDNA library was converted into a Bluescript phagemid cDNA library by in vivo excision by the ExAssist/SOLR system (Stratagene).

text: XL-1 blue

text: Rattus norvegicus

text: Eco RI

text: Xho I

text: Sprague-Dowley

text: Rat incisor (noncalcified tissues)

text: Plasmid

text: Bluescript II SK -


text: Smith College Department of Biological Sciences

text: live

text: Generic

text: Lymphatic filarial nematode parasite of humans. mRNA was prepared from adult males of Brugia malayi isolated from jirds and converted to double stranded cDNA using reverse transcriptase and oligo(dT) followed by RNase H and DNApol I. The library had 4.6 x 10E6 independent recombinants and average insert size was 800 base pairs. The library was constructed by Noelle Ling. The library is available from Dr. S.A. Williams, email genome@smith.edu.

text: XL1-Blue MRF'

text: Brugia malayi

text: EcoR I

text: Xho I

text: TRS Labs

text: Brugia malayi adult male cDNA (SAW94NL-BmAM)

text: Phage

text: lambda UniZap XR


text: live

text: Generic

text: Lymphatic filarial nematode parasite of humans. mRNA was prepared from third stage infective larvae of Brugia malayi isolated from mosquitoes 10 days after infection and converted to double stranded cDNA using reverse transcriptase and oligo(dT) followed by RNase H and DNApol I. The library had 1.6 x 10E6 independent recombinants and average insert size was 900 base pairs. The library was constructed by Wenhong Lu. The library is available from Dr. S.A. Williams, email genome@smith.edu.

text: XL1-Blue MRF'

text: Brugia malayi

text: EcoR I

text: Xho I

text: TRS Labs

text: Brugia malayi infective larva cDNA (SAW94WL-BmL3)

text: Phage

text: lambda UniZap XR


text: live

text: Generic

text: Lymphatic filarial nematode parasite of humans. mRNA was prepared from microfilariae of Brugia malayi isolated from jirds and converted to double stranded cDNA using reverse transcriptase and oligo(dT) followed by RNase H and DNApol I. The library had 3.5 x 10E5 independent recombinants and average insert size was 900 base pairs. The library was constructed by Lori Saunders. The library is available from Dr. S.A. Williams, email genome@smith.edu.

text: XL1-Blue MRF'

text: Brugia malayi

text: EcoR I

text: Xho I

text: TRS Labs

text: Brugia malayi microfilaria cDNA (SAW94LS-BmMf)

text: Phage

text: lambda UniZap XR


text: live

text: Generic

text: Cutaneous filarial nematode parasite of humans. mRNA was prepared from third stage infective larvae of Onchocerca volvulus isolated from mosquitoes 10 days after infection and converted to double stranded cDNA using reverse transcriptase and oligo(dT) followed by RNase H and DNApol I. The library had 1.8 x 10E5 independent recombinants and average insert size was 900 base pairs. The library was constructed by Wenhong Lu. The library is available from Dr. S.A. Williams, email genome@smith.edu.

text: XL1-Blue MRF'

text: Onchocerca volvulus

text: EcoR I

text: Xho I

text: Sierra Leone

text: Onchocerca volvulus infective larva cDNA (SAW94WL-OvL3)

text: Phage

text: lambda UniZap XR


text: Harvard Medical School

text: live

text: Generic

text: mRNA was purified from human fetal hearts (8-10 weeks). cDNA was synthesized using a XhoI-Oligo dT adaptor-primer. EcoRI adaptors were ligated, followed by digestion with XhoI, for directional cloning into predigested lambda ZAP Express.

text: E. coli XL1-Blue

text: Homo sapiens

text: EcoRI

text: XhoI

text: Human fetal heart, Lambda ZAP Express

text: Phage

text: Lambda ZAP Express


text: California Institute of Technology

text: live

text: Generic

text: mRNA was isolated from LPS activated coelomocytes. cDNA was constructed with random primer containing a Not1 site, and an EcoR1 adapter was ligated at the 5' end. The cDNA was directionally cloned.

text: Strongylocentrotus purpuratus

text: coelomocyte

text: Phage

text: lambda ExCell


text: University of Tokyo

text: live

text: Generic

text: Exon-like sequence on chromosome 8; exon-trapping method

text: Homo sapiens

text: Human chromosome 8


text: University of Colorado Health Sciences Center

text: live

text: Generic

text: The mouse brain library (Stratagene ) was constructed by oligo-(dT) priming and directional cloning in Uni-ZAP XR phage using whole brain mRNA from a Balb C post natal 20 day.

text: E. coli XL1-Blue

text: Mus musculus

text: EcoR I

text: Xho I

text: Mouse brain, Stratagene

text: Phage

text: Lambda ZAP


text: live

text: Generic

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae mRNA


text: CNRS, Institut de Biotechnologie des Plantes

text: live

text: Generic

text: Physiological condition: nitrate treated liquid-cultured A.thaliana. tissue_type: roots

text: Arabidopsis thaliana

text: Ors-B

text: Lambda ZAPII


text: Massachusetts General Hospital

text: live

text: Generic

text: Exons were isolated from human chromosome 21 specific cosmids using a modification of the method of exon amplification (Proc. Natl. Acad. Sci. USA 88:4005-4009, 1991). Amplified exons were digested with SalI and BamHI and subsequently cloned into pBluescriptIIKS+ at the SalI and BglII sites.

text: E. coli DH5a

text: Homo sapiens

text: Chromosome 21 exon

text: Plasmid

text: pBluescriptIIKS+


text: Institut National de la Recherche Agronomique

text: live

text: Generic

text: granulosa

characteristic: cell type

text: immature

text: Sus scrofa

text: female

text: ovary

characteristic: tissue-type

text: S.scrofa ovary


text: University of Florida

text: live

text: Generic

text: Small intestine mRNA from zinc deficient and zinc adequate rats was analyzed by mRNA differential display as described by Liang et al (Nucl. Acids Res. 21:3269, 1993). This produced 3' ESTs to genes that are regulated by dietary zinc deficiency. The pri ers used are not included in the EST sequence but are listed in the comments. The sequences are reported in 5' to 3' orientation relative to the poly A tail / anchor primer.

text: One Shot Top 10F'

text: Rattus norvegicus

text: EcoRI

text: EcoRI

text: Sprague-Dawley

text: Zinc regulated rat small intestine

text: pCR-II

characteristic: vector


characteristic: External Id

text: live

text: Generic

text: developmental-stage=embryonic day 17 post-fertilization cells and adult cells. tissue-type=aorta. cell-type=vascular smooth muscle cells grown in vitro. sex=male

text: Rattus norvegicus

text: Sprague-Dawley

text: differential mRNA display


text: Kyungpook National University

characteristic: INSDC center name

text: live

text: Generic

text: Blunt-ended cDNA was digested with Sau3A, size-selected, and then was cloned into BamHI site of BamHI/HincII site of M13mp19.

text: Brassica napus

text: BamHI

text: HincII

text: cv. Naehan

text: Phage


text: Kyungpook National University

characteristic: INSDC first public

text: live

text: Generic

text: cDNA library was constructed by oligo(dT) priming and directionally cloned into HincII/EcoRI site of M13mp19.

text: Brassica napus

text: HincII

text: EcoRI

text: cv. Naehan

text: Phage


text: Queen's Medical Centre

characteristic: INSDC last update

text: live

text: Generic

text: Exons were isolated from human chromosome 19p12-p13.1 specific cosmids from Lawrence Livermore National Laboratory using a modification of the method of exon amplification (Proc. Natl. Acad. Sci. USA 88: 4005-4009, 1991). Amplified exons were cloned into pAMP10 by uracial cloning (GIBCOL BRL).

text: E. coli DH5a

text: Homo sapiens

text: Chromosome 19p12-p13.1 exon

text: Plasmid


text: Washington University School of Medicine

text: live

characteristic: INSDC status

text: Generic

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCCCTTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of a modified pT7T3 vector (Pharmacia). Library went through one round of normalization to a Cot = 20. Library constructed by Bento Soares and M.Fatima Bonaldo.

text: adult

text: DH10B (ampicillin resistant)

text: breast

text: Homo sapiens

text: Not I

text: Eco RI

text: Female

characteristic: sex

text: Soares breast 3NbHBst

text: phagemid

text: pT7T3D (Pharmacia) with a modified polylinker


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCGCTTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was size selected, ligated to Eco RI adapters (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of a modified pT7T3 vector (Pharmacia). Library went through one round of normalization to a Cot = 53. Library constructed by Bento Soares and M.Fatima Bonaldo. The adult brain RNA was provided by Dr. Donald H. Gilden. Tissue was acquired 17-18 hours after death which occurred in consequence of a ruptured aortic aneurysm. RNA was prepared from a pool of tissues representing the following areas of the brain: frontal, parietal, temporal and occipital cortex from the left and right hemispheres, subcortical white matter, basal ganglia, thalamus, cerebellum, midbrain, pons and medulla.

text: 55-year old

text: DH10B (ampicillin resistant)

text: brain

text: Homo sapiens

text: Not I

text: Eco RI

text: Male

text: Soares adult brain N2b4HB55Y

text: phagemid


text: live

text: Generic

characteristic: description

text: 55-year old

text: DH10B (ampicillin resistant)

text: brain

text: Homo sapiens

characteristic: organism

text: Not I

text: Eco RI

text: Male

text: Soares adult brain N2b5HB55Y

text: phagemid


text: University of Cambridge

text: live

text: Generic

text: Human adult testis (Nucl. Acids Res. 17:2987 1989)

text: Homo sapiens

text: human adult testis

characteristic: title


text: live

text: Generic

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCGCTTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was size selected, ligated to Eco RI adapters (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of a modified pT7T3 vector (Pharmacia). The retinas were obtained from a 55 year old Caucasian and total cellular poly(A)+ RNA was extracted 6 hrs after their removal. The retina RNA was kindly provided by Roderick R. McInnes M.D. Ph.D. from the University of Toronto. Library constructed by Bento Soares and M.Fatima Bonaldo.

text: 55 year old

text: DH10B (ampicillin resistant)

text: eye

characteristic: organ

text: Homo sapiens

text: Not I

text: Eco RI

text: male

text: retina

text: Soares retina N2b4HR

text: phagemid


text: live

text: Generic

text: 55 year old

text: DH10B (ampicillin resistant)

characteristic: lab-host

text: eye

text: Homo sapiens

text: Not I

text: Eco RI

text: male

text: retina

text: Soares retina N2b5HR

text: phagemid


text: The Institute for Genomic Research

text: live

text: Generic

text: poly(A)+ RNA was purified from 9-day NGF treated PC12 cells. cDNA was constructed using an oligo-dT primer and directionally cloned using the Lambda ZAP II Vector Kit by Stratagene

text: Rattus norvegicus

text: EcoRI

text: XhoI

text: Rat PC-12 cells, NGF-treated (9 days)

text: Phagemid

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: poly(A)+ RNA was purified from untreated PC12 cells cultured for 9 days. cDNA was constructed using an oligo-dT primer and directionally cloned using the Lambda ZAP II Vector Kit by Stratagene

text: Rattus norvegicus

text: EcoRI

text: XhoI

text: Rat PC-12 cells, untreated

text: Phagemid

text: pBluescript SK-


text: Rutgers University

text: live

text: Generic

text: cDNA from IFNgamma-induced cultured rat astrocytes was screened by PCR differential display. Regulated cDNAs were amplifed, sequenced and confirmed by dot-blots and Northerns.

text: Rattus norvegicus

text: Sprague-Dawley

characteristic: strain

text: IFNgamma-induced astrocyte ESTs


text: Pacific Biomedical Research Center, University of Hawaii

text: live

text: Generic

text: Urechis caupo

text: Urechis caupo cDNA


text: University of Southern California

text: live

text: Generic

text: Poly(A)+ RNA was purified from a 97% pure population of osteoclasts prepared from the long bones of 10 day old rabbits. First strand cDNA was synthesized by priming with an oligo(dT)-NotI anchor-primer and second strand cDNA was synthesized by replacement synthesis as described by Gubler and Hoffman (Gene 25:283, 1983). Following the addition of SalI adapters and NotI digestion, the cDNA was cloned between the SalI (5o) and NotI (3o) sites of the pSPORT1 (BRL) plasmid vector.

text: E. coli DH12S

text: Oryctolagus cuniculus

text: SalI

text: NotI

text: New Zealand White

text: Rabbit Osteoclast, Dennis Sakai

text: Plasmid


text: live

text: Generic

text: Developmental stage = d 10.5 p.c.

text: Mus musculus

text: BALB/c x C57BL/6

text: Mouse cDNA

text: lambda ZAP II


text: Fred Hutchinson Cancer Research Center

text: live

text: Generic

text: Male adult, hematopoietic tissue, stem cell

text: Homo sapiens

text: Human stem cell


text: DKFZ (German Cancer Research Center)

text: live

text: Generic

text: Hydra vulgaris

text: Hydra vulgaris lambda ZAPII

text: pBluescript II SK+


text: Otsuka Pharmaceutical Co.,Ltd

text: live

text: Generic

text: Homo sapiens

text: Clontech human aorta polyA+ mRNA (#6572)


text: NIH

text: live

text: Generic

text: Full length cDNA was prepared by RT- PCR from mRNA from vector-stage infective L4 larvae of the human filarial nematode parasite Brugia malayi using nematode spliced leader (SL, 5' end) and oligo-d(T) (3' end) primers. The library had an unamplified titre of 6.4 x 10E5 per ml and 94% of clones have inserts (mean length ~700 bp)

text: XL1-Blue MRF-

text: Brugia malayi

text: XbaI

text: EcoRI

text: Brugia malayi infective L4 JHU93SL-BmL4

text: Uni-Zap XR


text: Experimental Therapeutics

text: live

text: Generic

text: cDNA subtraction library prepared from aflatoxin B1 transformed C3H10T1/2 cells minus parental C3H10T/2 cells

text: Mus musculus

text: Mouse fibroblast C3H10T1/2


text: Institute of Basic Medical sciences, Peking Union Medical College & Chinese Academy of Medical Sciences

text: live

text: Generic

text: Homo sapiens

text: Human fetal brain


text: Theodor Bilharz Research Institute

text: live

text: Generic

text: mRNA was purified from adult couples of S. mansoni. cDNA was constructed and cloned simultaneously using vector priming with the pBluescript II SK+ vector. cDNA was directionally synthesized from the EcoR1 site in the vector to the Xho1 site.

text: E. coli XL Blue1

text: Schistosoma mansoni

text: Egyptian

text: S. mansoni cDNA

text: pBluescript II SK+


text: Otsuka Pharmaceutical Co.,Ltd

text: live

text: Generic

text: Homo sapiens

text: Clontech human fetal brain polyA+ mRNA (#6535)


text: University of Pennsylvania

text: live

text: Generic

text: Mus musculus

text: Mouse (G.O.Babalola)


text: Otsuka Pharmaceutical Co.,Ltd

text: live

text: Generic

text: Homo sapiens

text: Clontech human placenta polyA+ mRNA (#6572)


text: Otsuka Pharmaceutical Co.,Ltd

text: live

text: Generic

text: Homo sapiens

text: Clontech human placenta polyA+ mRNA (#6518)


text: The Natural History Museum

text: live

text: Generic

text: Total mRNA was isolated from adult worms using the acid guanidine thiocyanate method (Chomczynski and Sacchi, 1987). Poly (A)+ RNA was purified by affinity chromatography on oligo dT column (Aviv and Leder, 1972). The cDNA expression library was constructed using the Lambda Zap cDNA cloning system (Stratagene-[Uni-Zap XR Gigapack II cloning kit). The library was amplified (end titre=0.5 x 10E8 pfu/ml) and its efficiency tested for using known S.mansoni probes (chorion and tropomyosin genes). The size range of inserts for the library was 0.5Kb - 2.5 Kb, the cDNA being directionally cloned between the EcoRI and the XhoI sites of the Bluescript phagemid. Bluescript phagemid was excised by mass in vivo excision of the library and inserted into E.coli XL1 Blue. The plasmid was purified using the Qiagen Qiawell 8 system and sequenced with Taq dye-terminator chemistry (SK,T3 and T7 primers) for ABI 373A. The library was prepared by Hanan Abdel Hamed, Ain Shams Univ.,Cairo and provided by Mohamed Saber, Theodore Bilharz Research Institute, Cairo.

text: Golden Hamster

text: Schistosoma mansoni

text: EcoRI

text: XhoI

text: Egyptian

text: SmAW

text: Bluescript


text: Department of Animal Science and Animal Health, Division of Animal Genetics, The Royal Veterinary and Agricultural University

text: live

text: Generic

text: directionally cloned cDNA in XL1-blue MRF'

text: Sus scrofa

text: Porcine small intestine cDNA library


text: live

text: Generic

text: A mini-library was made by cloning products derived from RNA-arbitrarily primed PCR (RAP PCR) profiles into the pUC 18 vector. Reverse transcription of adult worm mRNA was primed with LA1 and subsequent PCR amplification was performed in the presence of primer LA2

text: Schistosoma mansoni

text: LA1/LA2 adult worm mini-library


text: live

text: Generic

text: A mini-library was made by cloning products derived from RNA-arbitrarily primed PCR (RAP PCR) profiles into the pUC 18 vector. Reverse transcription of adult worm mRNA was primed with 3303 and subsequent PCR amplification was performed in the presence of primer TCZ2

text: Schistosoma mansoni

text: 3303/TCZ2 adult worm mini-library


text: live

text: Generic

text: A mini-library was made by cloning products derived from RNA-arbitrarily primed PCR (RAP PCR) profiles into the pUC 18 vector. Reverse transcription of adult worm mRNA was primed with C2and subsequent PCR amplification was performed in the presence of primer C2

text: Schistosoma mansoni

text: C2/C2 adult worm mini-library


text: live

text: Generic

text: A mini-library was made by cloning products derived from RNA-arbitrarily primed PCR (RAP PCR) profiles into the pUC 18 vector. Reverse transcription of lung-stage schistosomula mRNA was primed with C2 and subsequent PCR amplification was performed in the presence of primer G1

text: Schistosoma mansoni

text: C2/G1 lung-stage schistosomula mini-library


text: live

text: Generic

text: A mini-library was made by cloning products derived from RNA-arbitrarily primed PCR (RAP PCR) profiles into the pUC 18 vector. Reverse transcription of adult worm mRNA was primed with C2and subsequent PCR amplification was performed in the presence of primer tcz2

text: Schistosoma mansoni

text: C2/tcz2 adult worm mini-library


text: live

text: Generic

text: A mini-library was made by cloning products derived from RNA-arbitrarily primed PCR (RAP PCR) profiles into the pUC 18 vector. Reverse transcription of lung stage schistosomula mRNA was primed with G1 and subsequent PCR amplification was performed in the presence of primer C2

text: Schistosoma mansoni

text: G1/C2 Lung-stage schistosomula mini-library


text: live

text: Generic

text: A mini-library was made by cloning products derived from RNA-arbitrarily primed PCR (RAP PCR) profiles into the pUC 18 vector. Reverse transcription of adult worm mRNA was primed with G1and subsequent PCR amplification was performed in the presence of primer G1

text: Schistosoma mansoni

text: G1/G1 adult worm mini-library


text: live

text: Generic

text: A mini-library was made by cloning products derived from RNA-arbitrarily primed PCR (RAP PCR) profiles into the pUC 18 vector. Reverse transcription of 3h schistosomula mRNA was primed with G1 and subsequent PCR amplification was performed in the presence of primer G2

text: Schistosoma mansoni

text: G1/G2 3h schistosomula mini-library


text: live

text: Generic

text: A mini-library was made by cloning products derived from RNA-arbitrarily primed PCR (RAP PCR)profiles into the pUC 18 vector. Reverse transcription of adult worm mRNA was primed with G1 and subsequent PCR amplification was performed in the presence of primer G2

text: Schistosoma mansoni

text: G1/G2 adult worm mini-library


text: live

text: Generic

text: A mini-library was made by cloning products derived from RNA-arbitrarily primed PCR (RAP PCR) profiles into the pUC 18 vector. Reverse transcription of adult worm mRNA was primed with G2and subsequent PCR amplification was performed in the presence of primer G2

text: Schistosoma mansoni

text: G2/G2 adult worm mini-library


text: live

text: Generic

text: A mini-library was made by cloning products derived from RNA-arbitrarily primed PCR (RAP PCR) profiles into the pUC 18 vector. Reverse transcription of adult worm mRNA was primed with G2and subsequent PCR amplification was performed in the presence of primer KS

text: Schistosoma mansoni

text: G2/KS adult worm mini-library


text: live

text: Generic

text: A mini-library was made by cloning products derived from RNA-arbitrarily primed PCR (RAP PCR) profiles into the pUC 18 vector. Reverse transcription of lung-stage schistosomula mRNA wasprimed with G2 and subsequent PCR amplification was performed in the presence of primer SK

text: Schistosoma mansoni

text: G2/SK lung-stage schistosomula mini-library


text: live

text: Generic

text: A mini-library was made by cloning products derived from RNA-arbitrarily primed PCR (RAP PCR) profiles into the pUC 18 vector. Reverse transcription of adult worm mRNA was primed with C2and subsequent PCR amplification was performed in the presence of primer 25RY

text: Schistosoma mansoni

text: C2/25RY adult worm mini-library


text: live

text: Generic

text: A mini-library was made by cloning products derived from RNA-arbitrarily primed PCR (RAP PCR) profiles into the pUC 18 vector. Reverse transcription of adult worm mRNA was primed with C2and subsequent PCR amplification was performed in the presence of primer SK

text: Schistosoma mansoni

text: C2/SK adult worm mini-library


text: live

text: Generic

text: A mini-library was made by cloning products derived from RNA-arbitrarily primed PCR (RAP PCR) profiles into the pUC 18 vector. Reverse transcription of adult worm mRNA was primed with tcz3 and subsequent PCR amplification was performed in the presence of primer ks

text: Schistosoma mansoni

text: tcz3/ks adult worm mini-library


text: FDA-CBER

text: live

text: Generic

text: J774 macrophage cell line

text: Mus musculus

text: Mouse macrophage WRSchwan


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCGGTTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was size selected, ligated to Eco RI adapters (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of a modified pT7T3 vector (Pharmacia). Library constructed by Bento Soares and M.Fatima Bonaldo.

text: DH10B (ampicillin resistant)

text: ovary

text: Homo sapiens

text: Not I

text: Eco RI

text: Female

text: ovarian tumor

text: Soares ovary tumor NbHOT

text: phagemid

text: pT7T3D (Pharmacia) with a modified polylinker


text: Department of Genetics, The Hospital for Sick Children

text: live

text: Generic

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCCGTTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was size selected, ligated to Eco RI adapters (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of a modified pT7T3 vector (Pharmacia). Library constructed by Bento Soares and M.Fatima Bonaldo.

text: DH10B (ampicillin resistant)

text: pineal gland

text: Homo sapiens

text: Not I

text: Eco RI

text: phagemid


text: Texas A&M University

text: live

text: Generic

text: mRNA was purified from cotton developing embryos and a directional cDNA (directionally cloned with Sal1 and Not1) was constructed in pSPORT 1 according to the manufacturer's instructions (Life Technologies, Inc.).

text: DH10B (Life Technologies, Inc.)

text: Gossypium hirsutum

text: Coker 312

text: Cotton embryo

text: Plasmid

text: pSPORT 1 (Life Technologies, Inc)


text: Clontech Laboratories, Inc.

text: live

text: Generic

text: Testis cDNAs were subtracted with a mixture of 10 cDNAs derived from 10 other human tissues using a method based on a newly developed suppression polymerase chain reaction (PCR) technique (to be published). This method amplifies only cDNA sequences that are unique to testis and arrests the PCR amplification of sequences that are common to the different tissue samples. After the subtraction, PCR products representing the subtracted cDNAs were inserted by TA cloning into the pCRII vector (Invitrogen).

text: Homo sapiens

text: Testis, Subtracted

text: Plasmid

text: pCRII


text: Centre National de Recherches Scientifiques (CNRS)

text: live

text: Generic

text: using an oligodT-XhoI primer. After second strand synthesis, an EcoRI adaptator was ligated and the cDNA was size selected on an agarose gel before directional cloning in the EcoRI and XhoI sites of lamda Zap vector.

text: Homo sapiens

text: EcoRI

text: XhoI

text: Phage

text: lambda zap


text: Yale University Medical School

text: live

text: Generic

text: mRNA was purified from the ocular ciliary body of an adult female donor. A unidirectional cDNA library was constructed using lambda-Uni-ZAP XR vector (Stratagene, La Jolla, Ca) as described by Escribano et al. (J. Biochem. 1995). Using lambda DNA from the cDNA library as template and the set of primers indicated a 417-bp product was obtained, directly subcloned into the TA Cloning vector (pCR II) (Invitrogen Corp., San Diego, Ca), and further sequenced from the 5' and 3' ends. Forward Primer: ACGGTCGGATCCAGTGTTTCA Reverse Primer: AGGCGGGCTTGTTCTCCAT

text: Homo sapiens

text: EcoRI

text: EcoRI

text: Human ocular ciliary body cDNA library 1

text: TA


text: Yale University Medical School

text: live

text: Generic

text: mRNA was purified from the ocular ciliary body of an adult female donor. A unidirectional cDNA library was constructed using lambda-Uni-ZAP XR vector (Stratagene, Ca) as described by Escribano et al. (J. Biochem. 1995). Using lambda DNA from the cDNA library as template and the set of primers indicated a 412-bp product was obtained, directly subcloned in the TA Cloning vector (pCR II) (Invitrogen Corp., San Diego, Ca), and further sequenced from the 5' and 3' ends. Forward Primer: CTACCAGACGGGCTCAGTGTTG Reverse Primer: GGGCCAGCTTGTCAGTCTCCT

text: Homo sapiens

text: EcoRI

text: EcoRI

text: Human ocular ciliary body cDNA library 2

text: TA


text: Eleanor Roosevelt Institute for Cancer Research

text: live

text: Generic

text: cDNAs were selected from pooled cDNA libraries, (fetal brain, whole fetus, and adult testes, thymus, liver, and spleen), by hybridization to YACs which map to chromosome 21q

text: Homo sapiens

text: EcoRI

text: Chromosome 21, K. Gardiner

text: Phage

text: Lambda


text: Institut de Biologie Moleculaire des Plantes

text: live

text: Generic

text: The Plant Journal (1992) 2(3), 417-422

text: E. coli MR 32

text: Arabidopsis thaliana

text: NotI

text: NotI

text: Arabidopsis thaliana cDNA library in yeast Saccharomyces cerevisiae, Francois Lacroute

text: Plasmid


text: Heinrich-Heine-University

text: live

text: Generic

text: leaf cDNA library

text: Sorghum bicolor

text: cv. Golden Harvest

text: Sorghum bicolor cv. Golden Harvest

text: Phage

text: Lambda gt11


text: Heinrich-Heine-University

text: live

text: Generic

text: leaf cDNA library, unidirectionally cloned

text: Sorghum bicolor

text: cv. TX430

text: Sorghum bicolor cv. TX430

text: Phage

text: Lambda ZAP II


text: Massachusetts General Hospital

text: live

text: Generic

text: Exons were isolated from human chromosome 22 specific cosmids using a modification of the method of exon amplification (Proc. Natl. Acad. Sci. USA 88:4005-4009, 1991). Amplified exons were digested with Sal I and Bgl II and subsequently cloned into pBluescriptIIKS+ at the Sal I and Bam HI sites.

text: E. coli DH5a

text: Homo sapiens

text: Sal I

text: Bam HI (destroyed)

text: Chromosome 22 exon

text: Plasmid

text: pBluescriptIIKS+


text: live

text: Generic

text: cDNAs were obtained by reciprocal hybridization of an arrayed placenta cDNA library and a chromosome 21 cosmid library as in Lee et.al., Human Molecular Genetics (1995) 4:1373-1380.

text: Homo sapiens

text: NotI

text: 21q Placenta, F. Tassone and K. Gardiner

text: Plasmid

text: Bluescript


text: Center for Human Genetics

text: live

text: Generic

text: Homo sapiens

text: Human chromosome 12p cDNAs


text: University of Melbourne

text: live

text: Generic

text: Mouse myeloid cell line NFS-60

text: Mus musculus

text: Mouse NFS-60


text: National University of Singapore

text: live

text: Generic

text: mRNA was purified from mixed stages of zebrafish embryos from 6 hours postfertilization to hatching. cDNA was unidirectionally cloned into the lambda ZAPII vector using Stratagene's Lambda Uni-ZAP XR cDNA cloning system

text: Danio rerio

text: EcoRI

text: XhoI

text: zebrafish embryonic

text: Phage

text: Lambda Uni-ZAP XR


text: Departamento de Parasitologia, Microbiologia e Imunologia, Universidade de Sao Paulo (USP)

text: live

text: Generic

text: The cDNA library of Schistosoma mansoni was provided by CLONTECH (Cambridge Bioscience, England). Poly (A)+ RNA was purified from male and female adult worms. The cDNA was constructed and cloned in lambda gt11 vector and method described by HUYNH, TU et al.(in DNA Cloning Techniques: A practical approach. D. Glover, ed. IRL Press. Oxford. 1985). Double-stranded cDNA were passed through a Sepharose 4B column to remove cDNA which are smaller than 600 bp. cDNAs in the range between 600 bp and 3.6 kb were cloned into the EcoRI site of lambda gt11.

text: Y1088, Y1090

text: Schistosoma mansoni

text: EcoRI

text: LE

text: Schistosoma mansoni, Lambda gt11, Clontech

text: Phage

text: Lambda gt11


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Olfactory epithelium, normal. Average insert size: 0.8 kb; Uni-ZAP XR Vector. Library constructed by N. Walker, D. Lancet, Weizmann Institute of Science. ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'

text: 35 year old

characteristic: dev-stage

text: SOLR cells (kanamycin resistant)

text: nose

text: Homo sapiens

text: EcoRI

text: XhoI

text: Female

text: olfactory epithelium

text: Weizmann Olfactory Epithelium

text: phagemid

text: pBluescript SK-


text: CNRS

text: live

text: Generic

text: Brown adipose tissue perirenal, children. Purification of polyA mRNA, first strand priming with oligodT primers, dGdC tailing and cloning between the PstI and BamHI sites of pTZ18 vector.

text: Homo sapiens

text: PstI

text: BamHI

text: caucasian


text: Pohang University of Science and Technology

text: live

text: Generic

text: ESTs were generated from the root cDNA library of Brassica napus. The cDNA was synthesized from root poly(A) RNA with Pharmacia's cDNA synthesis kit using oligo(dT) as a primer. The Eco RI/Not I adaptor was ligated to the dsDNA. The cDNAs inserts were cloned into the pUC19 vector digested with EcoRI.

text: E. coli DH5a

text: Brassica napus

text: EcoRI

text: EcoRI

text: L. cv Naehan

text: Standard

text: Plasmid


text: live

text: Generic

text: ESTs were generated from the root cDNA library of Brassica napus. The cDNAs were primed with oligo(dT) primer. Before inserting the cDNAs into the pUC19 vector digested with SmaI, we deleted approximately 200 nucleotides from both 5' and 3' ends to remove some of the noncoding region of cDNAs. The SmaI site in pUC19 was destroyed by cloning.

text: E. coli DH5a

text: Brassica napus

text: SmaI

text: SmaI

text: L. cv Naehan

text: Deletion-treated

text: Plasmid


text: live

text: Generic

text: ESTs were generated from the root cDNA library of Brassica napus. The cDNAs were primed with random hexamer and were cloned into the M13mp18 vector digested with Eco RI.

text: E. coli XL-1 Blue

text: Brassica napus

text: EcoRI

text: EcoRI

text: L. cv Naehan

text: Random-primed


text: Institute of Forest Genetics

text: live

text: Generic

text: The tissue source for this library is seedlings. The cDNA was randomly primed and not directionally cloned.

text: Pinus taeda

text: Plasmid


text: Stanford University

text: live

text: Generic

text: Arabidopsis thaliana

text: Arabidopsis thaliana M.W.Schena


text: Max-Planck-Institut fuer Biochemie

text: live

text: Generic

text: RT-PCR from mouse epithelial cell line PYS-2

text: Mus musculus

text: Mouse RT-PCR I.Oberbaeumer


text: live

text: Generic

text: From adult human thymus NSTH II cells; randomly primed; normalized

text: Homo sapiens

text: Human thymus NSTH II


text: live

text: Generic

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCAGTTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was size selected, ligated to Eco RI adapters (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of a modified pT7T3 vector (Pharmacia). Library constructed by Bento Soares and M.Fatima Bonaldo. RNA from normal foreskin melanocytes (FS374) was kindly provided by Dr. Anthony P. Albino.

text: DH10B (ampicillin resistant)

text: Homo sapiens

text: Not I

text: Eco RI

text: Male

text: melanocyte

characteristic: tissue-type

text: Soares melanocyte 2NbHM

text: phagemid

text: pT7T3D (Pharmacia) with a modified polylinker


text: live

text: Generic

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCGATTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was size selected, ligated to Eco RI adapters (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of a modified pT7T3 vector (Pharmacia). Library constructed by Bento Soares and M.Fatima Bonaldo.

text: two placentae: one from 8 weeks and another from 9 weeks post conception

text: DH10B (ampicillin resistant)

text: placenta

text: Homo sapiens

text: Not I

text: Eco RI

text: phagemid

characteristic: vector


characteristic: External Id

text: Western General Hospital

text: live

text: Generic

text: library from adult mouse testis

text: Mus musculus

text: Mouse adult male testis


text: Institute of Biomedical Sciences, Academia Sinica

characteristic: INSDC center name

text: live

text: Generic

text: cDNA cloned following differential display

text: Angiostrongylus cantonensis

text: Angiostrongylus cantonensis differential display


text: Brigham and Women's Hospital

characteristic: INSDC first public

text: live

text: Generic

text: Reference: Genomics 23, 42-50 (1994) Cloned unidirectionally. Primer: Oligo dT. Fetal cochlea, normal. 37% of inserts <0.5 kb, 56% 0.5-1.0 kb, 7% >1 kb. Uni-ZAP XR Vector. Library constructed by N. Robertson, C. Morton. ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'

text: 16-22 week fetus

text: SOLR cells (kanamycin resistant)

text: ear

text: Homo sapiens

text: EcoRI

text: XhoI

text: cochlea

text: Morton Fetal Cochlea

text: phagemid (ampicillin resistant)

text: pBluescript SK-


text: Department of Morphogenesis, National Institute for Basic Biology

characteristic: INSDC last update

text: live

text: Generic

text: Gallus gallus

text: Chicken lens fibers


text: Universita' di Parma

text: live

characteristic: INSDC status

text: Generic

text: mRNA was purified from the root of sulfate deprived maize seedlings. cDNA was constructed using anchored oligo(dT) primers, and PCR amplified in the presence of the same anchored oligo(dT) primer and random primer (Science 257:967-971). Amplified cDNA fragments were ligated into the pMOSBlue vector (Amersham).

text: Zea mays

text: EcoRV

text: Root, Angelo Bolchi

text: pMOSBlue


text: Osaka University

text: live

text: Generic

text: Adult female, liver

text: adult

text: liver

text: Mus musculus

text: female

characteristic: sex

text: Mouse liver 3'-directed


text: Osaka University

text: live

text: Generic

characteristic: NCBI submission model

text: day 6.5-8.5 of gestation

text: Mus musculus

text: decidual tissue

text: Mouse 3'-directed


text: Institute of Molecular Biology and Biotechnology, FORTH

text: live

text: Generic

characteristic: NCBI submission package

text: K562 (erythro-leukemic)

characteristic: cell-line

text: K562-cDNA library normalization: Puzyrev et al., Molecular Biology (Moleculyarnaya Biologiya), 29:58-61(1995)

text: embryonal/fetal with respect to globin expression

text: Homo sapiens

text: Human normalized K562-cDNA


text: Beijing Institute of Radiation Medicine

text: live

text: Generic

text: myeloid leukemia HL-60

text: Human lymphocyte cell line HL60

characteristic: description

text: E.coli JM109

text: Homo sapiens

characteristic: organism

text: Human HL60

text: Phagemid

text: pGEM-T


text: live

text: Generic

text: mRNA was purified from 5 x 10E5 filariform larvae which had been isolated from experimentally infected dogs. cDNA was constructed and, using adaptors, was cloned unidirectionally into the vector from the EcoRI site to the XhoI site. The library has an unamplified titer of 3 x 10E5 pfu/ml and an amplified, undiluted titer of 1.5 x 10E10 pfu/ml. The average insert size of the unamplifed library is 850 bp (range, 200-1500).

text: XL-1 Blue MRF' (Stratagene)

text: Strongyloides stercoralis

text: EcoRI

text: XhoI

text: Filariform larvae obtained from dogs

characteristic: title

text: Phage

text: Lambda Uni-ZAP XR (Stratagene)


text: Universita' di Parma

text: live

text: Generic

text: mRNA was purified from in vitro cultured tomato cotyledons on MS medium added with 1,2 -benzisooxazole-acetic acid plus zeatine riboside. cDNA was constructed using anchored oligo(dT) primers, and PCR amplified in the presence of the same anchored oligo(dT) primer and random primer (Science 257:967-971). Amplified cDNA fragments were ligated into the pCR II vector (Invitrogen).

text: Solanum lycopersicum

text: EcoRI

text: BZ shoot

text: pCR II


text: Universita' di Parma

text: live

text: Generic

text: mRNA was purified from tomato cotyledons after 7 days from sowing. cDNA was constructed using anchored oligo(dT) primers, and PCR amplified in the presence of the same anchored oligo(dT) primer and random primer (Science 257:967-971). Amplified cDNA fragments were ligated into the pCR II vector (Invitrogen).

text: Solanum lycopersicum

text: EcoRI

text: t0 cot

text: pCR II


text: Washington University School of Medicine

text: live

text: Generic

text: mRNA was purified from 4 x 10E5 filariform larvae which had been isolated from infected humans. cDNA was constructed and, using adaptors, was cloned unidirectionally into the vector from the EcoRI site to the XhoI site. The library has an unamplified titer of 1.5 x 10E6 pfu/ml and an amplified, undiluted titer of 7 x 10E9 pfu/ml. The average insert size of the unamplifed library is 975 bp (range, 500-1500).

text: XL-1 Blue MRF' (Stratagene)

characteristic: lab-host

text: Strongyloides stercoralis

text: EcoRI

text: XhoI

text: Filariform larvae obtained from humans

text: Phage

text: Lambda Uni-ZAP XR (Stratagene)


text: live

text: Generic

text: mRNA was purified from 2 x 10E3 rhabditiform larvae which had been isolated from gerbils experimentally infected with larvae originally isolated from experimentally infected dogs. cDNA was constructed and, using adaptors, was cloned unidirectionally into the vector from the EcoRI site to the XhoI site. The library has an unamplified titer of 1 x 10E5 pfu/ml and an amplified, undiluted titer of 9 x 10E11 pfu/ml. The average insert size of the unamplifed library is 675 bp (range, 100-1700).

text: XL-1 Blue MRF' (Stratagene)

text: Strongyloides stercoralis

text: EcoRI

text: XhoI

text: Rhabditiform larvae obtained from gerbils

characteristic: strain

text: Phage

text: Lambda Uni-ZAP XR (Stratagene)


text: University of California

text: live

text: Generic

text: Hordeum vulgare subsp. vulgare

text: Willis

text: Hordeum vulgare TJClose


text: University of Padua

text: live

text: Generic

text: The library was constructed by G. Lanfranchi. This library is not subtracted nor normalized. The first strand cDNA was primed with a biotinylated oligo-dT-NotI primer~(5'-biotin-AACCCGGCTCGAGCGGCCGCTTTTTTTTTTTTTTTTTT-3'). The ds cDNA was sonicated and size-selected in the range 250-400 bp. The 3' specific fragments were selected by streptavidin coated magnetic beads, ligated to non-palindromic BstXI adapters, NotI digested and directionally cloned into BstXI-NotI cut pcDNAII vector.

text: Homo sapiens

text: EcoRI

text: NotI

text: female

text: pectoral muscle (after mastectomy)

text: Plasmid

text: pcDNAII (Invitrogen)


text: School of Veterinary Medicine, Institute for Parasitology

text: live

text: Generic

text: dev_stage=third stage larvae; specific_host=Bos taurus

text: Dictyocaulus viviparus

text: DvL3 PCR-cDNA


text: University of Tokyo

text: live

text: Generic

text: Adult male cerebral cortex tissue.

text: Homo sapiens

text: Human cerebral cortex


text: Cancer Institute, CAMS, PUMC

text: live

text: Generic

text: Homo sapiens

text: Human


text: Animal Sciences, University of Maryland

text: live

text: Generic

text: Bos taurus placentome; dev_stage=90 day

text: Bos taurus

text: Bos taurus placentome


text: Anna University

text: live

text: Generic

text: The cDNA library of the B.malayi L4 stage was constructed at Johns Hopkins University. The presence of a conserved 22 nucleotide splice leader at 5'end of large proportion of mature messages was exploited in the linked Reverse transcriptase-polymerase chain reaction in the construction of this librry. The library had unamplified phage titre of 6.4X10E5 pfu per ml and 94% of the clones have inserts (mean length approx 700 bp). Acknowledgements: Nithyakalyani Raghavan, Inca Ghosh, Ward Eisinger, Alan Scott Dept. of Molecular Microbiology and Immunology School of Public health and Hygiene, Johns Hopkins University, 615, N.Wolfe street,Baltimore MD 21205, USA. Tel 410 955 3442 Fax: 410 955 0105

text: XL-1 Blue MRF'

text: Brugia malayi

text: Xba I

text: EcoR I

text: JHU93SL-BmL4 (JHU)

text: Phage

text: Uni-ZAP XR


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCATTTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was size selected, ligated to Eco RI adapters (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of a modified pT7T3 vector (Pharmacia). Library went through one round of normalization to a Cot = 5. Library constructed by Bento Soares and M.Fatima Bonaldo. RNA from 4 multiple sclerosis lesions from one patient was kindly provided by Dr. Kevin G. Becker (NINDS/NIH).

text: Age 46

characteristic: dev-stage

text: DH10B (ampicillin resistant)

text: Homo sapiens

text: Not I

text: Eco RI

text: male

text: multiple sclerosis lesions

text: pT7T3D (Pharmacia) with a modified polylinker V_TYPE: phagemid


text: Centre d'Immunologie INSERM/CNRS

text: live

text: Generic

text: The cDNA library was constructed from poly(A)+ RNA of an adult mouse thymus by oligo-dT primed reverse transcription. cDNA was selected on gel for size above 800 nucleotides after second strand synthesis, then directionally cloned after adaptor ligation and restriction enzyme cleavage into the pcDNA1 vector (Not 1 on polyA side, EcoR 1 on the 5'side).

text: MC1061 p3

text: Mus musculus

text: MTA adult mouse thymus library

text: Plasmid


text: Institute for Neurobiology

text: live

text: Generic

text: isolated from expression cDNA library with antisera against synaptic protein preparation

text: postnatal day 25

text: brain

characteristic: organ

text: Rattus norvegicus

text: Sprague-Dawley

text: Rat brain, postnatal day 25

text: phage

text: lambda gt11


text: University of Edinburgh

text: live

text: Generic

text: Double stranded cDNA was prepared by PCR amplification of first strand, oligo(dT) primed cDNA prepared from poly(A) plus RNA from day fourteen post-infection fourth stage larvae isolated from the jird host. Unamplified titer: 6.4 x 105 pfu/ml; Amplified titer: 2 x 109 pfu/ml; Background: < 6% non-recombinants; Average insert size: ~ 700 bp; Transcripts: 98 % full length transcripts; Primers used in library construction: 5' end: XSL: 5' GCT CTA GAG CGG TTT AAT TAC CCA AGT TTG AG 3' 3' end: XET: 5' GCG CGC TCG AGA ATT CTT TTT TTT TTT TTT TTT TTT 3'. The library was made by Inca Ghosh, Nithyakalyani Raghavan, Ward Eisinger and Alan Scott (email ascott@hpcsun01.sph.jhu.edu).

text: Brugia malayi

text: Xba I (5' end)

text: Eco RI (3' end)

text: TRS Labs

text: Brugia malayi L4 JHU93SL-BmL4

text: Phage

text: Uni-Zap XR

characteristic: vector


characteristic: External Id

text: Otsuka Pharmaceutical Co.,Ltd

text: live

text: Generic

text: fetal

text: brain

text: Homo sapiens

text: Human fetal brain (TFujiwara)


text: Otsuka Pharmaceutical Co.,Ltd

characteristic: INSDC center name

text: live

text: Generic

text: Homo sapiens

text: aorta

characteristic: tissue-type

text: Human aorta polyA+ (TFujiwara)


text: Otsuka Pharmaceutical Co.,Ltd

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Homo sapiens

text: placenta

text: Human placenta polyA+ (TFujiwara)


text: University of Wisconsin-Madison

text: live

characteristic: INSDC status

text: Generic

text: Islets were cultured in the presence of a high concentration of glucose (20mM) in RPMI 1640 medium for 24 hours. Expression of these genes appears to be increased 2-3 fold by culture at high glucose.

text: XL-1 Blue

text: Rattus norvegicus

text: Sprague-Dawley

text: pancreatic islet

text: Rat pancreatic islet

text: Phage

text: Lambda ZAP II


text: Institute of Basic Medical sciences, Peking Union Medical College & Chinese Academy of Medical Sciences

text: live

text: Generic

characteristic: NCBI submission model

text: sequence obtained by DDRT-PCR

text: fetus

text: brain

text: Homo sapiens

text: Human fetal brain QBoqin


text: Ludwig Institute of Cancer Research

text: live

text: Generic

characteristic: NCBI submission package

text: IRF-1 deficient mouse embryonic fibroblast

text: embryonic

text: Mus musculus

text: fibroblast

text: Mouse embryonic fibroblast


text: Institute of Physiology, University of Zuerich-Irchel

text: live

text: Generic

text: hepatoma

characteristic: cell type

characteristic: cell-line

text: liver cell line

text: Homo sapiens

text: Human hepatoma RHWenger


text: Molecular and Cell Biology, University of California at Berkeley

text: live

text: Generic

text: fibroblast

text: Homo sapiens

characteristic: organism

text: fibroblast

text: Human fibroblast cDNA


text: live

text: Generic

text: A mini-library was made by cloning products derived from RNA-arbitrarily primed PCR (RAP PCR) profiles into the pUC 18 vector. Reverse transcription of adult worm mRNA was primed with tcz2 and subsequent PCR amplification was performed in the presence of primer KS

characteristic: description

text: adult

text: Schistosoma mansoni

text: tcz2/KS adult worm mini-library

characteristic: title

text: plasmid


text: live

text: Generic

text: A mini-library was made by cloning products derived from RNA-arbitrarily primed PCR (RAP PCR) profiles into the pUC 18 vector. Reverse transcription of day seven schistosomula mRNA was primed with C2 and subsequent PCR amplification was performed in the presence of primer S1

text: day seven schistosomula

text: Schistosoma mansoni

text: C2/S1 day seven schistosomula mini-library

text: plasmid


text: Internal Medicine I, University of Ulm

text: live

text: Generic

text: Patu 8988t

text: Homo sapiens

text: pancreatic cancer

text: Human pancreatic cancer cell line Patu 8988t


text: Chinese Academy of Medical Sciences & Peking Union Medical College

text: live

text: Generic

text: sequence obtained by DDRT-T-PCR

text: Rattus norvegicus

text: cerebellum

text: Rat R2 cerebellum DDRT-T-PCR


text: Johns Hopkins University School of Medicine

text: live

text: Generic

text: brain

text: Homo sapiens

text: frontal cortex

text: Human frontal cortex, Stratagene

text: phage

text: Lambda ZAP II