text: University of Milan

text: live

text: Generic

text: Fiesta

text: Total RNA was isolated from fruit flesh (a pool of early stage, middle stage and ripening stage fruits) and from young leaves according to Zeng and Yang (Plant Molecular Report 20:417a-417e, 2002). Poly(A)+mRNA extracted from leaves and poly(A)+mRNA from fruit flesh were used to construct a fruit versus leaf subtractive library employing the PCR-Select cDNA Subtraction kit (Clontech), using fruit cDNA as tester and leaf cDNA as driver, to enrich for fruit specific genes. cDNA fragments were cloned into the pGEM-T easy plasmid vector (Promega). The ligated cDNA fragments were transformed into E.coli JM109E host cells.

text: JM109E cells

text: fruit

characteristic: organ

text: Malus domestica

text: fruit flesh

text: plasmid

text: pGEM-T easy vector (Promega)


status: PUBLIC

characteristic: External Id

text: University of Milan

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Fiesta

tag: attribute

characteristic: cultivar

text: Total RNA was isolated from fruit flesh (a pool of early stage, middle stage and ripening stage fruits) according to Zeng and Yang (Plant Molecular Report 20:417a-417e, 2002). Poly(A)+mRNA extracted from fruit flesh was used to construct a subtractive library employing the PCR-Select cDNA Subtraction kit (Clontech) using fruit cDNA as tester and driver, to reduce redundancy. cDNA fragments were cloned into pGEM-T easy plasmid vector (Promega). The ligated cDNA fragments were transformed into E.coli JM109E host cells.

characteristic: description

text: JM109E cells

characteristic: lab-host

text: fruit

text: Malus domestica

characteristic: organism

text: fruit flesh

characteristic: tissue-type

characteristic: title

text: plasmid

text: pGEM-T easy vector (Promega)

characteristic: vector


text: State Key Laboratory for Biocontrol, Sun Yat-sen (Zhongshan) University

text: live

text: Generic

text: The research of the autopsy sample was after informed consent by the patient's family members and in accordance with Helsinki principles. The first-strand cDNA was generated by using random hexamer primers. double-stranded cDNA was ligated into pGEM-T-Easy (Promega) and transformed into E. coli cell DH10B.

text: autopsy sample, immediately after the death of a SARS patient

characteristic: dev-stage

text: lung

text: Homo sapiens

text: male

characteristic: sex

text: lung

text: SARS-Cov infected lung tissue

text: Plasmid

text: pGEM-T-Easy (Promega)


text: USDA-ARS

text: live

text: Generic

text: Greehouse-reared colony. A high quality EST with at least 150 contiguous bases at KB basecaller score of 20 or better. Library construction by Christine Lynch (USDA-ARS-USHRL).

text: Adult

text: Diaphorina citri

text: EcoRI

text: XhoI

text: Male

text: testes

text: Asian citrus psyllid testes (Hemiptera: Psyllidae)

text: pBluescript II SK+


text: Rubber Research Institute of India

text: live

text: Generic

text: clone RRII105

text: Cells from healthy and TPD affected trees. Sequences generated by DDRT-PCR method.

text: rubber tree 18 year old

text: Hevea brasiliensis

text: bark tissues

text: cDNAs prepared from inner bark tissues of rubber tree


text: Universidade Estadual de Londrina

text: live

text: Generic

text: adult

text: Gallus gallus

text: breast muscle

text: Chicken breast muscle Library


text: East China Normal University

text: live

text: Generic

text: Daphnia carinata

text: Body

text: Parthenogenetic water flea (Daphnia carinata) cDNA library

text: Plasmid

text: pBluescript SK+


text: Rubber Research Institute of India

text: live

text: Generic

text: Latex vessel cells from healthy rubber tree

characteristic: cell type

text: clone RRII105

text: Total RNA was isolated from latex cells of 18 year old field-grown rubber trees. Following preparation of latex mRNA, subsequent steps of cDNA synthesis and library construction were performed using the ZAP-cDNA Synthesis Kit and ZAP-cDNA Gigapack II Gold Cloning Kit (Stratagene). Latex cDNAs cloned in the Lambda Uni-Zap XR vector were amplified once from the packaging reaction. Subsequently, Lambda Uni-Zap XR clones were in vivo excised into pBluescript phagemids for use as sequencing templates.

text: rubber tree 18 year old

text: Hevea brasiliensis

text: EcoRI

text: XhoI

text: cDNAs prepared from latex vessel cells of rubber tree

text: Lambda Uni-ZAP XR


characteristic: External Id

text: University of California, Los Angeles

text: live

text: Generic

text: Hammond's Dwarf Scarlet

text: Embryo-proper regions were micro-dissected from globular-stage embryos six days after pollination from greenhouse-grown plants [Weterings et al., Plant Cell 13, 2409-2425 (2001)]. Complementary DNA (cDNA) was generated from 150 nanograms of total RNA from Scarlet runner bean globular-stage embryo-proper regions using a SMART PCR cDNA Synthesis kit (Clontech) with 26 cycles of PCR amplification. The cDNA sample was digested with a restriction enzyme Sfi I (New England Biolabs). Five micrograms of Sfi I-digested cDNA sample was sent to Roche 454 Sequencing Core Facility for sequencing.

text: 6-days post-pollination

text: Embryo-Proper Region of Globular-Stage Embryos

characteristic: organ

text: Phaseolus coccineus

text: SfiIA

text: SfiIB

text: Scarlet Runner Bean globular-stage embryo-proper regions

text: Plasmid


text: University of California, Los Angeles

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: Hammond's Dwarf Scarlet

text: Suspensor regions were micro-dissected from globular-stage embryos six days after pollination from greenhouse-grown plants [Weterings et al., Plant Cell 13, 2409-2425 (2001)]. Complementary DNA (cDNA) was generated from 4.7 micrograms of total RNA from Scarlet runner bean globular-stage suspensor region using a SMART PCR cDNA Synthesis kit (Clontech) with 20 cycles of PCR amplification. The cDNA sample was digested with a restriction enzyme Sfi I (New England Biolabs). Five micrograms of the Sfi I-digested cDNA sample was sent to Roche 454 Sequencing Core Facility for sequencing.

text: 6-days post-pollination

text: Suspensor Region of Globular-Stage Embryos

text: Phaseolus coccineus

text: SfiIA

text: SfiIB

text: Scarlet Runner Bean globular-stage suspensor region

text: Plasmid


text: University of California, Los Angeles

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Hammond's Dwarf Scarlet

text: Suspensor regions were micro-dissected from globular-stage embryos six days after pollination from greenhouse-grown plants [Weterings et al., Plant Cell 13, 2409-2425 (2001)]. Complementary DNA (cDNA) was generated from 4.7 micrograms of total RNA from Scarlet runner bean globular-stage suspensor region using a SMART PCR cDNA Synthesis kit (Clontech) with 20 cycles of PCR amplification. The cDNA sample was digested with a restriction enzyme Sfi I (New England Biolabs) and then size-fractionated over the Chroma-spin column (Clontech). Fractions containing Sfi I-digested cDNA with size greater than 400 bp were pooled and precipitated. Five micrograms of the size-selected Sfi I-digested cDNA sample was sent to Roche 454 Sequencing Core Facility for sequencing.

text: 6-days post-pollination

text: Phaseolus coccineus

text: SfiIA

text: SfiIB

text: Scarlet Runner Bean globular-stage suspensor region size-fractionated

text: Plasmid


text: University of Tokushima

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Mus musculus

text: Thymic rudiment

text: Mus musculus Thymic rudiment E11.5


text: CMBI, NCMLS, Radboud University Nijmegen

text: live

text: Generic

text: isolation_source: Blaberus sp. var. Amsterdam. country: Netherlands

characteristic: description

text: Nyctotherus ovalis

text: Nyctotherus ovalis library (Ricard G)


text: MPI for Molecular Genetics

text: live

text: Generic

text: isolation_source: aquaculture fish. collection_date: 10-Oct-2004. collected_by: Juan Fuentes and Carla Viegas. Total RNA was extracted from 5 male specimens of weight ranging between 400 and 500 g. Collected tissue frozen in liq N2 and stored at -80C prior to RNA extraction. mRNA purification and concentration were performed using the Dynal's oligo-dT magnetic beads. Library construction was performed with Creator SMART cDNA library construction kit (Clontech) and the thermostable duplex-specific nuclease (Zhu et al., 2001). Initial LD PCR amplification of 22 cycles for enrichment of full length transcripts was verified on Agarose gels. cDNA sizes (>500 base pairs) were selected using filter columns based on agarose gels as described in the Clontech manual. Library contruction and sequencing were performed at the MPI of Molecular Genetics Berlin.

text: adult

text: Dicentrarchus labrax

text: male

text: corpuscles of Stannius


text: MPI for Molecular Genetics

text: live

text: Generic

text: Total RNA was extracted from shock-frozen tissue. Library construction was performed with Cloneminer (Invitrogen) starting from enriched mRNA. Funded by European Rat Tools for Functional Genomics (EURATools).

text: adult

text: Rattus norvegicus

text: male

text: BN/SsNHsd/Mcwi

characteristic: strain

text: aorta

text: etcmaor


text: MPI for Molecular Genetics

text: live

text: Generic

text: adult

text: Rattus norvegicus

characteristic: organism

text: male

text: BN/SsNHsd/Mcwi

text: fat

text: etcmfat


text: MPI for Molecular Genetics

text: live

text: Generic

text: adult

text: Rattus norvegicus

text: male

text: BN/SsNHsd/Mcwi

text: heart

characteristic: tissue-type

text: etcmhea


text: MPI for Molecular Genetics

text: live

text: Generic

text: adult

text: Rattus norvegicus

text: male

text: BN/SsNHsd/Mcwi

text: kidney

text: etcmkid

characteristic: title


text: MPI for Molecular Genetics

text: live

text: Generic

text: Total RNA was extracted from shock-frozen tissue. Library construction was performed using Smart technology(Clontech) and normalised(Trimmer, Evrogen) Funded by European Rat Tools for Functional Genomics (EURATools).

text: adult

text: Rattus norvegicus

text: male

text: BN/SsNHsd/Mcwi

text: aorta

text: etnoaor


text: MPI for Molecular Genetics

text: live

text: Generic

text: adult

text: Rattus norvegicus

text: male

text: BN/SsNHsd/Mcwi

text: fat

text: etnofat


text: MPI for Molecular Genetics

text: live

text: Generic

text: adult

text: Rattus norvegicus

text: male

text: BN/SsNHsd/Mcwi

text: heart

text: etnohea


text: MPI for Molecular Genetics

text: live

text: Generic

text: adult

text: Rattus norvegicus

text: male

text: BN/SsNHsd/Mcwi

text: kidney

text: etnokid


text: MPI for Molecular Genetics

text: live

text: Generic

text: environmental_sample; isolation_source: oxic zone (upper 2-mm soil layer) of flooded, unplanted rice paddy soil microcosms incubated for 1 day (early community succession) in the dark at 30C. Non-normalized cDNA library from flooded, unplanted rice paddy soil. Microcosms were used as the model system. This library contains randomly selected clones from the oxic (upper 2-mm soil layer) zone of 1-day-old (early community succession) flooded microcosms (rps001).

text: uncultured organism


text: MPI for Molecular Genetics

text: live

text: Generic

text: environmental_sample; isolation_source: anoxic zone (3-5 mm beneath surface) of flooded, unplanted rice paddy soil microcosms incubated for 1 day (early community succession) in the dark at 30C. Non-normalized cDNA library from flooded, unplanted rice paddy soil. Microcosms were used as the model system. This library contains randomly selected clones from the anoxic (3-5 mm beneath surface) zone of 1-day-old (early community succession) flooded microcosms (rps002).

text: uncultured organism


text: MPI for Molecular Genetics

text: live

text: Generic

text: environmental_sample; isolation_source: oxic zone (upper 2-mm soil layer) of flooded, unplanted rice paddy soil microcosms incubated for 70 days (late community succession) in the dark at 30C. Non-normalized cDNA library from flooded, unplanted rice paddy soil. Microcosms were used as the model system. This library contains randomly selected clones from the oxic (upper 2-mm soil layer) zone of 70-day-old (late community succession) flooded microcosms (rps003).

text: uncultured organism


text: MPI for Molecular Genetics

text: live

text: Generic

text: environmental_sample; isolation_source: anoxic zone (3-5 mm beneath surface) of flooded, unplanted rice paddy soil microcosms incubated for 70 days (late community succession) in the dark at 30C. Non-normalized cDNA library from flooded, unplanted rice paddy soil. Microcosms were used as the model system. This library contains randomly selected clones from the anoxic (3-5 mm beneath surface) zone of 70-day-old (late community succession) flooded microcosms (rps004).

text: uncultured organism


text: Bhabha Atomic Research Centre (BARC)

text: live

text: Generic

text: leaves

text: Brassica juncea

text: leaves

text: thiol treated Brassica juncea


text: University of Maine

text: live

text: Generic

text: In the presence of sprouted potato tubers, mycelial cultures of quinate- induced RHS 1AP and uninduced RHS 1AP were grown and poly(A)+ RNA was extracted. To prepare subtracted cDNAS, we used the BD Clontech PCR-SELECT cDNA subtraction kit (BD Clontech). Normalization of cDNA was checked using the BD Advantage 2 PCR enzyme kit (BD Biosciences Clontech) and primers specific for the R. solani (AG3) glyceraldehyde-3-phosphate dehydrogenase (GPDH) (GenBank AF339929) or the R. solani (AG 3) specific -tubulin gene sequence, provided by Dolores Gonz Lez (Laboratorio de Sistem Tica Molecular Instituto de Ecolog A, A. C., Mexico). cDNA from quinate-induced RHS 1AP was subtracted with excess CDNA from untreated RHS 1AP and was checked for normalization of the two constitutive genes, GPDH and -tubulin. In the case of the GPDH gene, the respective pcr product became slightly visible after 31 cycles of subtracted cDNA amplification, whereas copious PCR product was generated after 19 cycles of unsubtracted cDNA amplification. Similarly, the -tubulin gene PCR product became visible following 33 cycles of subtracted cDNA amplification, but after only 18 cycles of unsubtracted cDNA amplification. Subtracted cDNAS were cloned into a T/A-based vector (TOPO PCR cloning kit - Invitrogen). The Eppendorf Fastplasmid mini kit (Brinkmann) was used for plasmid purification, and DNA sequencing was conducted using an ABI3700 capillary DNA sequencer.

text: Rhizoctonia solani

text: RHS 1AP (AG 3)

text: Quinate-induced cDNA library of Rhizoctonia solani


text: Institute of Hydrobiology, Chinese Academy of Sciences

text: live

text: Generic

text: blastulae embryonic cells

text: method: suppression substrative hybridization

text: Carassius auratus

text: crucian carp blastulae embryonic cells subtracted cDNA library

text: Plasmid

text: pGEM-T

characteristic: vector


text: National Institute of Genetics

text: live

text: Generic

text: Gametophores were cultivated on sterile peat pellets (Jiffy-7; Jiffy Products International AS, Kristansand, Norway) for 1 to 1.5 months at 25C under continuous light. The plants were moved to 15C under 8-h light/16-h dark conditions to induce gametangia and cultivated for 11 to 26 days. Shoot tips with archegonia and antheridia were collected. Shoot tips with archegonia that were brown in color, which is an indicator of fertilization, were removed. 1st strand cDNA was primed with an oligo (dT) primer [GCGGCTGAAGACGGCCTATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded cDNA was ligated to a DraIII adaptor [GGCCUACUGG], digested and directionally cloned into distinct DraIII sites of the pME18S-FL3. Library constructed by Yutaka Suzuki and Sumio Sugano (University of Tokyo, Institute of Medical Science). synonym: Physcomitrella patens subsp. patens

text: Physcomitrium patens

text: DraIII

text: DraIII

text: Gransden

text: gametangia (shoot tip with antheridia and archegonia)

text: ppaa full-length cDNA library; antheridia and archgonia


text: National Institute of Genetics

text: live

text: Generic

text: Gametophores were cultivated on sterile peat pellets (Jiffy-7; Jiffy Products International AS, Kristansand, Norway) for 1 to 1.5 months at 25C under continuous light. The plants were moved to 15C under 8-h light/16-h dark conditions to induce gametangia. The gametophores were cultivated at 15C under the short day conditions for 31 to 53 days to collect sporophyte tissue during meiosis. Archegonial tissue was removed and only sporophytic tissue was collected. 1st strand cDNA was primed with an oligo (dT) primer [GCGGCTGAAGACGGCCTATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded cDNA was ligated to a DraIII adaptor [GGCCUACUGG], digested and directionally cloned into distinct DraIII sites of the pME18S-FL3. Library constructed by Yutaka Suzuki and Sumio Sugano (University of Tokyo, Institute of Medical Science). synonym: Physcomitrella patens subsp. patens

text: Physcomitrium patens

text: DraIII

text: DraIII

text: Gransden

text: green sporophytes

text: ppgs full-length cDNA library; green sporophytes


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 1.2-3kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed at DOE Joint Genome Institute (Walnut Creek, CA).

text: day 4 post innoc

characteristic: dev-stage

text: Emiliania huxleyi

text: SalI

text: NotI

text: CAHN Emiliania huxleyi CCMP1516 day 4 post innoculation L


text: DOE Joint Genome Institute

text: live

text: Generic

text: day 7 post innoc

text: Emiliania huxleyi

text: SalI

text: NotI

text: CAHO Emiliania huxleyi CCMP1516 day 7 post innoculation L


text: DOE Joint Genome Institute

text: live

text: Generic

text: day 14 post innoc

text: Emiliania huxleyi

text: SalI

text: NotI

text: CAHP Emiliania huxleyi CCMP1516 day 14 post innoculation L


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected >2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed at DOE Joint Genome Institute (Walnut Creek, CA).

text: day 7 post innoc

text: Emiliania huxleyi

text: SalI

text: NotI

text: CAHS Emiliania huxleyi CCMP1516 day 7 post innoculation M


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 0.6-1.2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed at DOE Joint Genome Institute (Walnut Creek, CA).

text: day 7 post innoc

text: Emiliania huxleyi

text: SalI

text: NotI

text: CAHT Emiliania huxleyi CCMP1516 day 7 post innoculation L


text: DOE Joint Genome Institute

text: live

text: Generic

text: day 7 post innoc

text: Emiliania huxleyi

text: SalI

text: NotI

text: CAHU Emiliania huxleyi CCMP1516 day 7 post innoculation M


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 2-4kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed at DOE Joint Genome Institute (Walnut Creek, CA).

text: day 4 post innoc

text: Emiliania huxleyi

text: SalI

text: NotI

text: CAHW Emiliania huxleyi CCMP1516 day 4 post innoculation M


text: DOE Joint Genome Institute

text: live

text: Generic

text: day 14 post innoc

text: Emiliania huxleyi

text: SalI

text: NotI

text: CAHX Emiliania huxleyi CCMP1516 day 14 post innoculation H


text: DOE Joint Genome Institute

text: live

text: Generic

text: day 14 post innoc

text: Emiliania huxleyi

text: SalI

text: NotI

text: CAHY Emiliania huxleyi CCMP1516 day 14 post innoculation H


text: DOE Joint Genome Institute

text: live

text: Generic

text: day 14 post innoc

text: Emiliania huxleyi

text: SalI

text: NotI

text: CAHZ Emiliania huxleyi CCMP1516 day 14 post innoculation M


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT)-primed cDNA and cloned into the pMCL200_cdna vector. PolyA RNA was primed with oligo(dT) primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'-CGGACGCGTGGG) and digested with NotI. cDNA was size selected >2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pMCL200_cDNA vector. Library was created and sequencing done by the DOE Joint Genome Institute (Walnut Creek, CA).

text: day 7 post innoc

text: Emiliania huxleyi

text: SalI

text: NotI

text: CAIA Emiliania huxleyi CCMP1516 day 7 post innoculation M


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT)-primed cDNA and cloned into the pMCL200_cdna vector. PolyA RNA was primed with oligo(dT) primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'-CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 1.2-2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pMCL200_cDNA vector. Library was created and sequencing done by the DOE Joint Genome Institute (Walnut Creek, CA).

text: day 7 post innoc

text: Emiliania huxleyi

text: SalI

text: NotI

text: CAIB Emiliania huxleyi CCMP1516 day 7 post innoculation L


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: phos starved media. The library was made from oligo(dT)-primed cDNA and cloned into the pSPORT1 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo(dT) primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3') ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'-CGGACGCGTGGG) and digested with NotI. cDNA was size selected >2.5k using 1.1% agarose gel electrophoresis, then ligated into NotI and SalI-digested pSPORT1 vector. Library was created and sequencing done by the DOE Joint Genome Institute (Walnut Creek, CA).

text: Emiliania huxleyi

text: SalI

text: NotI

text: CUPI Emiliania huxleyi CCMP1516 F/2 phos starved media H


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: phos starved media. The library was made from oligo(dT)-primed cDNA and cloned into the pSPORT1 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo(dT) primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3') ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'-CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 1.2-2.5k using 1.1% agarose gel electrophoresis, then ligated into NotI and SalI-digested pSPORT1 vector. Library was created and sequencing done by the DOE Joint Genome Institute (Walnut Creek, CA).

text: Emiliania huxleyi

text: SalI

text: NotI

text: CUPN Emiliania huxleyi CCMP1516 F/2 phos starved media M


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: phos starved media. The library was made from oligo(dT)-primed cDNA and cloned into the pSPORT1 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo(dT) primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3') ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'-CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 0.6-1.2k using 1.1% agarose gel electrophoresis, then ligated into NotI and SalI-digested pSPORT1 vector. Library was created and sequencing done by the DOE Joint Genome Institute (Walnut Creek, CA).

text: Emiliania huxleyi

text: SalI

text: NotI

text: CUPO Emiliania huxleyi CCMP1516 F/2 phos starved media L


text: University of Milan

text: live

text: Generic

text: Fiesta

characteristic: cultivar

text: Total RNA was isolated from 46 DAFB fruit flesh according to Zeng and Yang (Plant Molecular Report 20:417a-417e, 2002). Phagemid cDNA library was constructed using the LambdaZAP-CMV XR library construction kit (Stratagene).

text: XL1-blue MRF

characteristic: lab-host

text: 46 DAFB fruit

text: Malus domestica

text: fruit flesh

text: phage

text: Lambda ZAP-CMV XR phagemid vector (Stratagene)


characteristic: External Id

text: Indian Institute of Pulses Research, Kanpur

text: live

text: Generic

text: JG 315

text: Total RNA was isolated from chickpea leaves and used as a template for cDNA synthesis using the SMART cDNA system (Clontech, CA). cDNA with dA overhangs were cloned into pTZ57R/T(Fermentas). Recombinant plasmid DNA was transformed into XL1 blue competent cells and got sequenced.

text: Three weeks old

text: E.coli. XL-1 Blue

text: Leaves

text: Cicer arietinum

text: EcoRI

text: HindIII

text: Hermaphrodite

characteristic: sex

text: Wilt resistant chickpea leave library (JG 315)

text: plasmid


text: Huazhong Agricultural University

characteristic: INSDC center name

text: live

text: Generic

text: 'BH'

text: Library constructed using Clontech PCR-Select cDNA Subtraction kit.

text: Citrus sinensis

text: hermaphrodite

text: fruit

text: 'BH' (somaclonal variated plants of Skaggs Bonanza navel orange) SSH library


text: DOE Joint Genome Institute

characteristic: INSDC first public

text: live

text: Generic

text: In Vivo Conditions: log phase growth cells. The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 1.2-2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed at DOE Joint Genome Institute (Walnut Creek, CA).

text: Chlorella variabilis

text: SalI

text: NotI

text: CBPS Chlorella sp. NC64A EST library (L)


text: DOE Joint Genome Institute

characteristic: INSDC last update

text: live

text: Generic

text: In Vivo Conditions: log phase growth cells. The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 3-8kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed at DOE Joint Genome Institute (Walnut Creek, CA).

text: Chlorella variabilis

text: SalI

text: NotI

text: CBWC Chlorella sp. NC64A EST library (H)


text: DOE Joint Genome Institute

text: live

characteristic: INSDC status

text: Generic

text: In Vivo Conditions: log phase growth cells. The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 2-3kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed at DOE Joint Genome Institute (Walnut Creek, CA).

text: Chlorella variabilis

text: SalI

text: NotI

text: CBWF Chlorella sp. NC64A EST library (M)


text: DOE Joint Genome Institute

text: live

text: Generic

characteristic: NCBI submission model

text: In Vivo Conditions: Mycelial Injury. This library was made using poly A RNA and oligo dT primers with 5'-Biotin-GGCGGCCGCACAACTTTGTACAAGAAAGTTGGGT-(T)19-3'. cDNA was ligated to attB1 adapter (5'-TCGTCGGGGACAACTTTGTACAAAAAAGTTGG-3'), size fractionated using sephacryl S-500 HR columns, and recombined to pDONR222-Lib vector. Library constructed by Pedro Martinez (Langebio-Cinvestav, Mexico) using tissue from Dr. Alfredo Herrera-Estrella. Sequencing was performed at the DOE Joint Genome Institute (Walnut Creek, CA).

text: conidiation

text: Trichoderma atroviride

text: IMI 206040

text: whole organism

text: CBWT Trichoderma atroviride culture induced to conidiate by mycelial injury


text: DOE Joint Genome Institute

text: live

text: Generic

characteristic: NCBI submission package

text: In Vivo Conditions: Light Induction. This library was made using poly A RNA and oligo dT primers with 5'p GACTAGT TCTAGA TCGCGA GCGGCCGCCC (T)15-3'. cDNA was ligated to adapter (5'-tcgacccacgcgtccg-3'), size fractionated using sephacryl S-500 HR columns, and ligated to pEXP-AD502 vector. Library constructed by Pedro Martinez (Langebio-Cinvestav, Mexico) using tissue from Dr. Alfredo Herrera-Estrella. Sequencing was performed at the DOE Joint Genome Institute (Walnut Creek, CA).

text: vegetative mycelium

text: Trichoderma atroviride

text: SalI

text: NotI

text: IMI 206040

text: whole organism

text: CBYP Trichoderma atroviride culture induced to conidiate by exposure to light


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: Trichoderma was grown in presence of either Rhizoctonia solani or Sclerotium rolfsii and mycelia collected before contact and when contact occurred. This library was made using poly A RNA and oligo dT primers with 5'p GACTAGT TCTAGA TCGCGA GCGGCCGCCC (T)15-3'. cDNA was ligated to adapter (5'-tcgacccacgcgtccg-3'), size fractionated using sephacryl S-500 HR columns, and ligated to pEXP-AD502 vector. Library constructed by Pedro Martinez (Langebio-Cinvestav, Mexico) using tissue from Dr. C. Kubicek. Sequencing was performed at the DOE Joint Genome Institute (Walnut Creek, CA).

characteristic: description

text: vegetative mycelium

text: Trichoderma atroviride

text: SalI

text: NotI

text: IMI 206040

text: whole organism

text: CCAH Trichoderma atroviride mycoparasitism


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: Control grown in the dark. This library was made using poly A RNA and oligo dT primers with 5'p GACTAGT TCTAGA TCGCGA GCGGCCGCCC (T)15-3'. cDNA was ligated to adapter (5'-tcgacccacgcgtccg-3'), size fractionated using sephacryl S-500 HR columns, and ligated to pSPORT1 vector. Library constructed by Pedro Martinez (Langebio-Cinvestav, Mexico) using tissue from Dr. Alfredo Herrera-Estrella. Sequencing was performed at the DOE Joint Genome Institute (Walnut Creek, CA).

text: vegetative mycelium

text: Trichoderma atroviride

text: SalI

text: NotI

text: IMI 206040

characteristic: strain

text: whole organism

text: CBYT Trichoderma atroviride culture grown in complete darkness


text: USDA-ARS; Jamie Whitten Delta States Research Center

text: live

text: Generic

text: A high quality EST with at least 150 contiguous bases at Trace Tuner score of 20 or better. Library construction by Margaret (Meg) L. Allen, USDA, ARS, Stoneville, MS, USA.

text: egg

text: Chrysoperla rufilabris

characteristic: organism

text: both

text: whole body

text: Chrysoperla rufilabris developed embryos


text: Teagasc

text: live

text: Generic

text: SSH library generated after 1 week PEG induced drought stress using stress material as tester and control as driver.

text: Lolium perenne

text: Leaf

characteristic: tissue-type

text: LF

text: pGEM T-easy


text: Teagasc

text: live

text: Generic

text: Lolium perenne

text: Root

text: RT

characteristic: title

text: pGEM T-easy


text: Korea Forest Research Institute

text: live

text: Generic

text: SSH library generated with subtracted transcripts from the laminarin-treated poplar suspension cells

text: JM109 strain

text: Populus alba x Populus glandulosa

text: EcoRI

text: Suspension cell

text: Subtractive cDNA library from laminarin-treated poplar suspension cells

text: plasmid

text: pGEM-T easy

characteristic: vector


text: Washington University School of Medicine

text: live

text: Generic

text: pSPORT 1 Vector is ampicillin resistent, M13 reverse primer was used by us for sequencing of 5' parts of inserts; 3' parts of cDNAs contain long polyA tracks which makes sequencing from 3' direction complicated

text: Hydra vulgaris

text: Not I

text: Sal I

text: Hydra EST -Kiel 1

text: plasmid


text: live

text: Generic

text: Total RNA samples were prepared individually from shark regenerated liver, checked for quality, then pooled for construction by Creator SMART Kit (Clontech). Plasmids were isolated and inserts 5' end-sequenced by the laboratory of Beijing Genomics Institute (BGI).

text: Adult

characteristic: dev-stage

text: Chiloscyllium plagiosum

text: Liver

text: Shark liver regeneration

text: pDNR-LIB


text: Kunming University of Science and Technology

text: live

text: Generic

characteristic: cultivar

text: exposed to sunlight for 2,4,6,and 8 days respectively

text: fruit

characteristic: organ

text: Pyrus pyrifolia

text: percicarp

text: Yunnan Red Pear Percicarp Supression Substractive Hybridization cDNA Library

text: pGEM-T Easy Vector


characteristic: External Id

text: Department of Genetics, Stellenbosch University

text: live

text: Generic

text: Genes expressed in the vine mealybug, Planococcus ficus (Signoret). Source: Breeding colony obtained from ARC Infruitec-Nietvoorbij (Western Cape, South Africa) and maintained on Cucurbita moschata, L.

text: Mixed (nymphs - adults)

text: Planococcus ficus

text: SfiI

text: SfiI

text: Mixed sex

text: Whole body

text: Planococcus ficus Primary Library

text: Plasmid

text: pDNR-LIB


text: live

text: Generic

text: Mixed (nymphs - adults)

text: Planococcus ficus

text: SfiI

text: SfiI

text: Mixed sex

characteristic: sex

text: Whole body

text: Planococcus ficus Fractionated Library 1

text: Plasmid

text: pDNR-LIB


characteristic: INSDC center name

text: live

text: Generic

text: Mixed (nymphs - adults)

text: Planococcus ficus

text: SfiI

text: SfiI

text: Mixed sex

text: Whole body

text: Planococcus ficus Fractionated Library 2

text: Plasmid

text: pDNR-LIB


characteristic: INSDC first public

text: live

text: Generic

text: Mixed (nymphs - adults)

text: Planococcus ficus

text: SfiI

text: SfiI

text: Mixed sex

text: Whole body

text: Planococcus ficus Fractionated Library 3

text: Plasmid

text: pDNR-LIB


characteristic: INSDC last update

text: live

text: Generic

text: Mixed (nymphs - adults)

text: Planococcus ficus

text: SfiI

text: SfiI

text: Mixed sex

text: Whole body

text: Planococcus ficus Fractionated Library 4

text: Plasmid

text: pDNR-LIB


text: National Institute of Allergy and Infectious Diseases

text: live

characteristic: INSDC status

text: Generic

text: unidirectional library was constructed using long distance PCR (Smart cDNA kit) and Lambda TriplEx2 vector

text: adult female

text: salivary glands

text: Culex tarsalis

text: female

text: Adult female salivary gland cDNA library Ctarsg1

text: Phage

text: Lambda TriplEx2


text: School of Life Sciences, Hubei University

text: live

text: Generic

characteristic: NCBI submission model

text: biotype:II

text: 4th instar larvae

text: Nilaparvata lugens

text: midgut of larvae

text: Nilaparvata lugens midgut of larvae 4th instar larvae


text: MPI for Molecular Genetics

text: live

text: Generic

characteristic: NCBI submission package

text: otal RNA was extracted from 20 goldfish specimens of both sex. Collected tissue frozen in liq N2 and stored at -80C prior to RNA extraction. mRNA purification and concentration were performed using the Dynal's oligo-dT magnetic beads. Library construction was performed with Creator SMART cDNA library construction kit (Clontech) and the thermostable duplex-specific nuclease (Zhu et al., 2001). Initial LD PCR amplification of 22 cycles for enrichment of full length transcripts was verified on agarose gels. cDNA sizes (>500 base pairs) were selected using filter columns based on agarose gels as described in the Clontech manual. Library construction and sequencing were performed at the MPI of Molecular Genetics Berlin.

text: adult

text: Carassius auratus

text: mixed

text: olfactory epithelium


text: Tokyo University of Agriculture

text: live

text: Generic

text: A Thellungiella full-length cDNA library was constructed from a mixture of mRNA extracted from stress-treated plants and various tissues of Thellungiella halophila. Stress treatments; 3 week old plants were subjcted to various stress treatments, such as high-salinity (500 mM NaCl for 1, 2, 3, 7 and 14 d), cold (4 degrees C for 2, 4, 8 and 24 h), freezing (-6 degrees C for 1, 2, 4 or 8 h), and ABA treatments (50 uM ABA for 1, 2, 4 and 8 h) in addition plants were grown under standard conditions as a control. After the above stress treatments, rosetta leaves from each time point were pooled and mRNA was extracted. Various tissues; cauline leaf, root, flower, silique were collected from 7 to 10 week old plants. Mature seed was collected from 12 to 20 week old plants.

characteristic: description

text: Eutrema halophilum


text: College of Life Science, Nanjing Normal University

text: live

text: Generic

text: first crab stage

text: Eriocheir sinensis

text: Yangtse River

characteristic: strain

text: abdomen

text: Eriocheir sinensis abdomen first crab stage

text: Plasmid

text: pMD19-T Vector from TakaRa,Inc.


text: China Agricultural University

text: live

text: Generic

text: Drought-stressed leaves at the stage of tillering by cDNA-AFLP analysis

text: Tillering stage

text: Oryza sativa

characteristic: organism

text: AseI

text: TaqI

text: hermaphrodite

text: leaves

text: pGEM-T easy vectors


text: The Johns Hopkins University

text: live

text: Generic

text: mixed

text: Cyprinodon variegatus

text: whole embryos, whole larva and hepatic tissue of the adult fish

characteristic: tissue-type

text: Sheepshead Minnow cDNA Library


text: Department of Biology, East China Normal University, Shanghai

text: live

text: Generic

text: adult

characteristic: lab-host

text: Eriocheir sinensis

text: Eriocheir sinensis testis cDNA Library

characteristic: title

text: plasmid

text: pBluescriptII


text: live

text: Generic

text: Carassius auratus ssp. 'Pengze'

text: liver

text: Pengze Crucian Carp liver cDNA library


text: USDA-ARS

text: live

text: Generic

text: Isolate: Ascosphaera aggregate infected larvae. Total RNA was purified by TRIZOL Reagent (Gibco BRL Life Technologies); cDNA library was constructed using Creator SMART cDNA Library Construction Kit (Clontech) (cat. # 634903)

text: early 4th instar larvae

characteristic: dev-stage

text: Megachile rotundata

text: SfiIA

text: SfiIB

text: bee larvae

text: IB, Ascosphaera aggregate infected alfalfa leafcutting bee library

text: Plasmid

text: pDNR-LIB

characteristic: vector


text: Institute of Oceanology, Chinese Academy of Sciences

text: live

text: Generic

text: Adult

text: Portunus trituberculatus

text: female

text: eyestalk

text: swimming crab eyestalk Library

text: pDNR-Lib


text: Ghent University

text: live

text: Generic

text: cDNA-AFLP-generated library consisting of partial cDNA sequences from cell cycle synchronized cultures

text: vegetative division cycle

text: Seminavis robusta

text: - mating type

text: F1-8B, PAE 0097

text: Seminavis robusta vegetative division cDNA-AFLP library


text: The J. Craig Venter Institute

text: live

text: Generic

characteristic: cultivar

text: Tissues: drought-stressed leaves, roots and nodules (8 weeks post-inoculation with S. meliloti 2011); fungal-infected roots (3, 5, 7 days post-inoculation with P. omnivora of 4-week-old plant); salt-treated leaves and roots (1, 6 hours post-treatment with 100mM NaCl); cell culture (root-derived cell suspension with equal parts of unelicited and yeast-elicited cell cultures at 3 hrs post-elicitation), and untreated aerial tissues including cotyledons, terminal buds, leaves, petioles and developing pods. Total RNA isolated using TRIzol reagent. No DNaseI digestion was performed. Total RNAs were pooled together. Double strand cDNA were synthesized from pooled RNA using SMART technology (http://www.evrogen.com/technologies/SMART.shtml). The prepared cDNA was normalized by cDNA denaturation/reassociation, treatment by duplex-specific nuclease (DSN) and amplification of normalized fraction by PCR. The normalized cDNA was then digested with SfiI, size-fractioned, directionally ligated into pDNR-LIB (Clontech) and electroprated into GC10 competent cells (Gene Choice). RNA provided by Esther Gonzalez, Rao Uppalapati, Sue Miller, Carrol Vance, Florian Frugier, Vagner Benedito and Greg May.

text: Medicago truncatula

text: SfiIA

text: SfiIB

text: mixed

text: pDNR-LIB (Clontech)


characteristic: External Id

text: Baylor College of Medicine

text: live

text: Generic

text: TOP10 cells from Invitrogen

characteristic: cell-line

text: The library was made by Zach Smith and John Colburne, The Center for Genomics and Bioinformatics, Indiana University, 915 East Third Street, Bloomington, Indiana 47405

text: pupa

text: Nasonia vitripennis

text: Sfi 1A

text: Sfi 1B

text: male

text: Nasonia vitripennis Male Pupae

text: plasmid

text: pDNR-LIB


text: Baylor College of Medicine

characteristic: INSDC center name

text: live

text: Generic

text: TOP10 cells from Invitrogen

text: pupa

text: Nasonia vitripennis

text: Sfi 1A

text: Sfi 1B

text: female

characteristic: sex

text: Nasonia vitripennis Female Pupae

text: plasmid

text: pDNR-LIB


text: Baylor College of Medicine

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: TOP10 cells from Invitrogen

text: adult

text: Nasonia vitripennis

text: Sfi 1A

text: Sfi 1B

text: male

text: Nasonia vitripennis Adult Male

text: plasmid

text: pDNR-LIB


text: Baylor College of Medicine

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: TOP10 cells from Invitrogen

text: adult

text: Nasonia vitripennis

text: Sfi 1A

text: Sfi 1B

text: female

text: Nasonia vitripennis Adult Female

text: plasmid

text: pDNR-LIB


text: Centro de Investigacion en Alimentacion y Desarrollo A.C.

text: live

text: Generic

characteristic: NCBI submission package

text: The mRNA was isolated from Alternaria alternata strains adapted to lethal levels of 2-propenyl-isothiocyanate according to the protocol published by Islas-Flores et al., 2006 (Molecular Biotechnology 34(1): 45-50). The cDNAs were prepared using the SMART PCR cDNA synthesis kit and subtracted by SSH procedure (Clontech, Palo Alto CA). The diferentially expressed cDNAs were cloned into p-GEM-T Easy vector and cells of E. coli JM109 were transformed with them (Promega, Madison, WI). Rsa I was used to digest the recombinant clones and verify the presence and sizes of the inserted fragments. Sequenciation of fragments was carried out using using the M13 forward primer in the Genome Analysis and Technologic Core Facility of the University of Arizona at Tucson, AZ.

characteristic: description

text: Alternaria alternata

text: Rsa I

text: Rsa I

text: Mycelium

text: Alternaria alternata treated with 2-pITC library

text: plasmid

text: p-GEMT Easy vector


text: live

text: Generic

text: Adult

text: Portunus trituberculatus

characteristic: organism

text: female

text: blood

text: swimming crab blood Library

text: pDNR-Lib


text: RIKEN Tsukuba Institute

text: live

text: Generic

text: The soybean full-length cDNA library was constructed essentially as reported previously (Seki et al. (1998) Plant J. 15:707-720; Seki et al. (2002) Science 296:141-145). This clone is in a modified pBluescript vector.

text: plants at various developmental stages and environmental conditions

text: E. coli DH10B

text: Glycine max

text: modified pBluescript


text: live

text: Generic

text: Total RNA samples were prepared individually from leaf of Bruguiera gymnorrhiza treated with 25 NaCl, checked for quality, then pooled for construction by Creator SMART Kit (Clontech). Plasmids were isolated and inserts 5' end-sequenced by the laboratory of Beijing Genomics Institute (BGI).

text: 4 months-old

text: Bruguiera gymnorhiza

text: leaf

characteristic: tissue-type

text: cDNA library of Bruguiera gymnorrhiza

text: pDNR-LIB


text: Syngenta Ltd

text: live

text: Generic

text: Zymoseptoria tritici

characteristic: strain

text: Library 1


text: Syngenta Ltd

text: live

text: Generic

text: Zymoseptoria tritici

text: Library 10

characteristic: title


text: Syngenta Ltd

text: live

text: Generic

text: Zymoseptoria tritici

text: Library 2


text: Syngenta Ltd

text: live

text: Generic

text: Zymoseptoria tritici

text: Library 4


text: Syngenta Ltd

text: live

text: Generic

text: Zymoseptoria tritici

text: Library 7


text: Syngenta Ltd

text: live

text: Generic

text: Zymoseptoria tritici

text: Library 9


text: Syngenta Ltd

text: live

text: Generic

text: Zymoseptoria tritici

text: Library 3


text: Syngenta Ltd

text: live

text: Generic

text: Zymoseptoria tritici

text: Library 5


text: Syngenta Ltd

text: live

text: Generic

text: Zymoseptoria tritici

text: Library 8


text: Syngenta Ltd

text: live

text: Generic

text: Zymoseptoria tritici

text: Library 6


text: National Institute of Fruit Tree Science

text: live

text: Generic

text: Citrus unshiu

text: Citrus unshiu library (Endo,T.)


text: CNR-IGV

text: live

text: Generic

text: Pullrex

text: SSH procedure on RNAs extracted from flower buds in normal and heat-treated (HS) tomato plants. The obtained cDNAs were cloned into TA-vectors.

text: 4-8 week old plants

characteristic: dev-stage

text: Solanum lycopersicum

text: developing flower buds

text: Plasmid

text: TA cloning pCR2.1 vector Invitrogen


text: Penn State University

text: live

text: Generic

text: adult

text: Thorax

characteristic: organ

text: Melitaea cinxia

text: Thorax

text: McinxiaTnt

text: plasmid


text: Penn State University

text: live

text: Generic

text: adult

text: Thorax

text: Spodoptera frugiperda

text: Thorax

text: SfrugiperdaTnt

text: plasmid


text: Faculdade de Medicina de Ribeirio Preto da Universidade de Sao Paulo

text: live

text: Generic

text: SSH library was constructed following instructions from PCR Select cDNA Subtraction Kit(Clonetech, USA). This library was created to identify T.rubrum up-regulated genes during growth in lipidic source.

text: E.coli MOS Blue

characteristic: lab-host

text: Trichophyton rubrum CBS 118892

text: Trichophyton rubrum cDNA Library NMMR11

text: Plasmid

text: pGEM-T

characteristic: vector


characteristic: External Id

text: University of Washington

text: live

text: Generic

text: mixed

text: Caenorhabditis elegans

text: mixed

text: whole worm

text: Caenorhabditis elegans N2 whole worm mixed stages


characteristic: INSDC center name

text: live

text: Generic

text: Miyagawa wase

characteristic: cultivar

text: fruit rind separation stage

text: Citrus unshiu

text: albedo

text: ALP


characteristic: INSDC first public

text: live

text: Generic

text: Miyagawa wase

text: callus

text: Citrus unshiu

text: callus

text: PCC


characteristic: INSDC last update

text: live

text: Generic

text: Aoshima unshiu

text: 2 weeks after sowing

text: Citrus unshiu

text: root

text: RGP


text: live

characteristic: INSDC status

text: Generic

text: Miyagawa wase

text: 60 days after flowering

text: Citrus unshiu

text: juice sac

text: YJS


text: live

text: Generic

characteristic: NCBI submission model

text: Miyagawa wase

text: fruit maturity stage

text: Citrus unshiu

text: albedo

text: ALM


text: live

text: Generic

characteristic: NCBI submission package

text: Kankitsu Chukanbohon Nou 6 Gou

text: 8 weeks after flowering

text: Citrus nobilis x Citrus kinokuni

text: ovule

text: EGJ


text: live

text: Generic

text: Miyagawa wase

text: fruit developmental stage

text: Citrus unshiu

text: whole fruit

text: WFY


text: live

text: Generic

text: Miyagawa wase

text: anthesis

text: Citrus unshiu

text: anther

text: ANT


text: live

text: Generic

text: Miyagawa wase

text: anthesis

text: Citrus unshiu

text: ovary

text: OVA


text: live

text: Generic

text: Miyagawa wase

text: anthesis

text: Citrus unshiu

text: style

text: STG


text: live

text: Generic

text: Valencia

text: 1 month after flowering

text: Citrus sinensis

text: seed

text: VSS


text: live

text: Generic

text: Miyagawa wase

text: fruit developmental stage

text: Citrus unshiu

text: juice sac

text: FRI


text: live

text: Generic

text: Miyagawa wase

text: fruit maturity stage

text: Citrus unshiu

text: juice sac

text: FRM


text: live

text: Generic

text: Trovita

text: callus

text: Citrus sinensis

text: embryonic induction callus

text: EIC


text: live

text: Generic

text: Hyuganatsu

text: anthesis

text: Citrus tamurana

text: style

text: SHA


text: live

text: Generic

text: Aoshima unshiu

text: 2 weeks after sowing

text: Citrus unshiu

text: hypocotyl

text: SLG


text: live

text: Generic

text: Miyagawa wase

text: fruit coloring stage

text: Citrus unshiu

text: flavedo

text: BFC


text: live

text: Generic

text: Miyagawa wase

text: 30 days before flowering

text: Citrus unshiu

text: floral bud

text: FBI


text: live

text: Generic

text: Aoshima unshiu

text: 4 days after water absorption

text: Citrus unshiu

text: seedling

text: GSA


text: National Institute of Allergy and Infectious Diseases

text: live

text: Generic

text: Secretory

characteristic: cell type

text: PCR-based cDNA library made with SMART cDNA library construction kit from Clontech

characteristic: description

text: Adult

text: Salivary gland

text: Anopheles stephensi

text: Female

characteristic: sex

text: Salivary gland

text: Anopheles stephensi salivary gland library ASSG-1

text: Phage

text: Lambda TriplEx2


text: National Research Center

text: live

text: Generic

text: Giza 3

text: Vicia faba

characteristic: organism

text: roots

characteristic: tissue-type

text: DD-Faba-Orobanche


text: The Johns Hopkins University

text: live

text: Generic

text: Brain cDNA Library, Suppression Subtraction Hybridization PCR, enriched for differentially expressed transcripts resulting from oral exposure to cyclotrimethylenetrinitramine (RDX) and 2,6-dinitrotoluene (2,6-DNT).

text: Colinus virginianus

text: brain

text: quail brain cDNA library

characteristic: title


text: Central Food Technological Research Institute

text: live

text: Generic

text: Nanjanagudu Rasabale

text: Musa x paradisiaca

text: fruit

text: Musa paradisiaca cv. Nanjanagudu Rasabale fruit


text: University of Glasgow

text: live

text: Generic

text: embryo 8.5 dpc

characteristic: dev-stage

text: Mus musculus

characteristic: strain

text: embryo

text: Mouse embryo 8.5 dpc

text: pBluescript KSII-


text: Okazaki National Research Institutes

text: live

text: Generic

text: bacteriocyte

text: DH10B; Bacteriocytes were isolated from parthenogenetic adult aphids by dissection. Full-length enriched library was generated by cap-trapper method (Carninci et al. 2001)

text: adult

text: Acyrthosiphon pisum

text: female

text: Acyrthosiphon pisum adult bacteriocyte

text: pFLC-I

characteristic: vector


characteristic: External Id

text: Okazaki National Research Institutes

text: live

text: Generic

text: DH5-alpha; Plasmodium ookinetes were generated by in vitro culture of gametocytes (sexual stage parasites collected from parasite infected rats) and purified with density gradient centrifugation using NycoPrep (1.077 A; AXIS-SHIELD PoCAS). mRNAs were extracted using micro-mRNA extraction kit (Pharmacia). Directional cDNA libraries were constructed by oligo d(T) priming of poly(A)+ RNA into SalI and NotI sites of pSPORT 1 vector using the Superscript Plasmid System for cDNA Synthesis and Plasmid Cloning Kit (Life Technologies, Inc.)

text: ookinete

text: Plasmodium berghei ANKA

text: ANKA

text: Plasmodium berghei ookinete


text: RIKEN Institute

characteristic: INSDC center name

text: live

text: Generic

text: host: Reticulitermes speratus; isolation_source: termite gut

text: Reticulitermes speratus gut symbiotic protist library


text: The University of Tokyo

text: live

text: Generic

text: PL-TS-1(clade A3)

characteristic: cell-line

text: host: Tridacna squamosa; country: Palau

text: Symbiodinium sp. PL-TS-1

text: PL-TS-1 (axenic culture) cDNA library


text: Forestry and Forest Products Research Institute

characteristic: INSDC first public

text: live

text: Generic

text: DH10B; Mixture of male strobili at various developmental stages. The full-length cDNA library was constructed essentially as reported previously (Seki et al. (1998) Plant J. 15:707-720; Seki et al. (2002) Science 296:141-145). This clone is in a modified pBluescript vector.

text: Cryptomeria japonica

text: male strobilus

text: Cryptomeria japonica male strobilus


text: Institute for Molecular Bioscience

characteristic: INSDC last update

text: live

text: Generic

text: Gigapack III Gold Cloning Kit, Stratagene

text: Oldenlandia affinis

text: leaf

text: Oldenlandia affinis leaf cDNA library


text: Hunan Agricultural University, China

text: live

characteristic: INSDC status

text: Generic

text: full bloom

characteristic: lab-host

text: Artemisia annua

text: flower

text: Artemisia annua blooming flower subtractive cDNA Library

text: pGEM-T Easy


text: Linnaeus Plant Sciences Inc. co/ National Research Council of Canada, Plant Biotechnology Institute (NRC-PBI)

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

characteristic: cultivar

text: Primary library (directionally cloned) constructed using Superscript II RNase H- RT and pCMVSport6.1. Normalization conducted using proprietary subtraction technology (Invitrogen Inc).

text: Ricinus communis

text: Developing seed (endosperm and embryo), mixed stages II-IV

text: R. communis developing seed


text: University of California at Davis (UCD)

text: live

text: Generic

text: The normalized library was constructed from multiple sources of RNA from a single genotype using Trimmer-Direct kit. cDNAs were then size-fractionated, directionally cloned into a custom medium-copy vector and transformations made with three size classes (S-large, T-medium and U-small) to minimize size bias due to differential transformation efficiency. Details of each source of RNA and library construction can be obtained at http://cgpdb.ucdavis.edu/

characteristic: description

text: E.coli

text: Helianthus annuus

text: CCF(STU) sunflower

text: Plasmid

text: pBRcDNASfiAB


text: University of California at Davis (UCD)

text: live

text: Generic

text: Quentin Cronk, UBC Botanical Garden

text: The normalized library was constructed from multiple sources of RNA from a single genotype. Details of each source of RNA and library construction can be obtained at http://compgenomics.ucdavis.edu/

text: E.coli

text: Barnadesia spinosa

characteristic: organism

text: CCHS Espina

text: Plasmid

text: pDNR-Lib


text: University of California at Davis (UCD)

text: live

text: Generic

text: USDA accession number: PI508077

text: E.coli

text: Guizotia abyssinica

text: CCHT Niger Seed

text: Plasmid

text: pDNR-Lib


text: University of California at Davis (UCD)

text: live

text: Generic

text: Green Globe

text: The normalized library was constructed from multiple sources of RNA from a single genotype using Trimmer-Direct kit. cDNAs were then size-fractionated, directionally cloned into a custom medium-copy vector and transformations made with three size classes (U-large, X-medium and W-small) to minimize size bias due to differential transformation efficiency. Details of each source of RNA and library construction can be obtained at http://cgpdb.ucdavis.edu/

text: E.coli

text: Cynara cardunculus var. scolymus

text: CCP(UWX) Globe Artichoke

text: Plasmid

text: pBRcDNASfiAB


text: National Center for Genetic Engineering and Biotechnology

text: live

text: Generic

text: Testis of broodstock cultured shrimp

text: Penaeus monodon

text: male

characteristic: sex

text: testis

characteristic: tissue-type

text: Penaeus monodon testis


text: live

text: Generic

text: Vitellogenic ovaries of wild shrimp with 2nd stage of ovarian development (EST# start with OV-N-S01 or OV-N-N01), Ovaries of female broodstock shrimp having GSI = 5.689% and 1.434% (EST# start with OV-N-ST)

text: ovarian development stage

characteristic: dev-stage

text: Penaeus monodon

text: female

text: ovary

text: Penaeus monodon ovary

characteristic: title


text: Centro de Investigacion y de Estudios Avanzados del Instituto Politecnico Nacional, Departamento de Recursos del Mar

text: live

text: Generic

text: differential display (dd-PCR) product from adult liver

text: (4-5) months

text: Oreochromis niloticus

text: M

text: Liver

text: Oreochromis niloticus adult liver


text: Heilongjiang Bayi Agricultural University

text: live

text: Generic

text: A subtracted cDNA library from the ovaries of laying and prelaying geese

text: adult

text: Anser anser

text: female

text: Zi goose

characteristic: strain

text: ovary

text: Goose ovary subtracted cDNA library


characteristic: External Id

text: Embrapa Recursos Geneticos e Biotecnologia

text: live

text: Generic

text: BRA 000591

text: Brachiaria brizantha (A. Rich) Stapf cv. Marandu, tetraploid (2n=4x=36) facultative apomictic, cultivated in the field at Embrapa Genetic Resources and Biotechnology was used as plant material to construct the library. Ovaries at stages I and II of development (megasporogenesis) were isolated using stereoscope microscope from inflorescences of these plants and immediately frozen in liquid nitrogen after isolation. RNA was extracted using Trizol Reagent (Invitrogen, Carlsbad, CA, USA). Poly(A)+ RNA was extracted from 70 ug of total RNA using Dynabeads (Dynal). Full-length cDNA libraries were constructed using the SMART cDNA synthesis kit in pTriplEx2 vector (Clontech, Palo Alto, CA, USA). The plasmids were transformed in Escherichia coli XL1 Blue and white clones were recovered and inserts were sequenced from the 5'-end using PT2F2 PRIMER (5' GCG CCA TTG TGT TGG TAC CC 3').

text: Megasporogenesis

text: Ovary

characteristic: organ

text: Urochloa brizantha

text: SfiI

text: SfiI

text: hermaphrodite

text: plasmid

characteristic: vector


characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: BRA 000591

text: Brachiaria brizantha (A. Rich) Stapf cv. Marandu, tetraploid (2n=4x=36) facultative apomictic, cultivated in the field at Embrapa Genetic Resources and Biotechnology was used as plant material to construct the library. Ovaries at stage IV of development (megagametogenesis) were isolated using stereoscope microscope from inflorescences of these plants and immediately frozen in liquid nitrogen after isolation. RNA was extracted using Trizol Reagent (Invitrogen, Carlsbad, CA, USA). Poly(A)+ RNA was extracted from 70 ug of total RNA using Dynabeads (Dynal). Full-length cDNA libraries were constructed using the SMART cDNA synthesis kit in pTriplEx2 vector (Clontech, Palo Alto, CA, USA). The plasmids were transformed in Escherichia coli XL1 Blue and white clones were recovered and inserts were sequenced from the 5'-end using PT2F2 PRIMER (5' GCG CCA TTG TGT TGG TAC CC 3').

text: Megagametogenesis

text: Ovary

text: Urochloa brizantha

text: SfiI

text: SfiI

text: hermaphrodite

text: plasmid


characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: BRA 002747

text: Brachiaria brizantha, diploid (2n=2x=18) sexual, cultivated in the field at Embrapa Genetic Resources and Biotechnology was used as plant material to construct the library. Ovaries at stages I and II of development (megasporogenesis) were isolated using stereoscope microscope from inflorescences of these plants and immediately frozen in liquid nitrogen after isolation. RNA was extracted using Trizol Reagent (Invitrogen, Carlsbad, CA, USA). Poly(A)+ RNA was extracted from 70 ug of total RNA using Dynabeads (Dynal). Full-length cDNA libraries were constructed using the SMART cDNA synthesis kit in pTriplEx2 vector (Clontech, Palo Alto, CA, USA). The plasmids were transformed in Escherichia coli XL1 Blue and white clones were recovered and inserts were sequenced from the 5'-end using PT2F2 PRIMER (5' GCG CCA TTG TGT TGG TAC CC 3').

text: Megasporogenesis

text: Ovary

text: Urochloa brizantha

text: SfiI

text: SfiI

text: hermaphrodite

text: plasmid


text: live

text: Generic

characteristic: NCBI submission model

text: BRA 002747

text: Brachiaria brizantha, diploid (2n=2x=18) sexual, cultivated in the field at Embrapa Genetic Resources and Biotechnology was used as plant material to construct the library. Ovaries at stage IV of development (megagametogenesis) were isolated using stereoscope microscope from inflorescences of these plants and immediately frozen in liquid nitrogen after isolation. RNA was extracted using Trizol Reagent (Invitrogen, Carlsbad, CA, USA). Poly(A)+ RNA was extracted from 70 ug of total RNA using Dynabeads (Dynal). Full-length cDNA libraries were constructed using the SMART cDNA synthesis kit in pTriplEx2 vector (Clontech, Palo Alto, CA, USA). The plasmids were transformed in Escherichia coli XL1 Blue and white clones were recovered and inserts were sequenced from the 5'-end using PT2F2 PRIMER (5' GCG CCA TTG TGT TGG TAC CC 3').

text: Megasporogenesis

text: Ovary

text: Urochloa brizantha

text: SfiI

text: SfiI

text: hermaphrodite

text: plasmid


text: University of St Andrews

text: live

text: Generic

characteristic: NCBI submission package

text: E. coli

characteristic: lab-host

text: Salmo salar

text: muscle

text: Forward subtracted library from fast myotomal muscle of Atlantic salmon


text: University of St Andrews

text: live

text: Generic

text: E. coli

text: Salmo salar

text: Fast muscle

text: Reverse subtracted library from fast myotomal muscle of Atlantic salmon


text: University of St Andrews

text: live

text: Generic

text: E. coli

text: Salmo salar

text: Fast muscle

text: Forward subtracted library from fast muscle of Atlantic salmon


text: The University of Melbourne

text: live

text: Generic

text: The library was constructed by Daniella Smith at the Queensland Institute of Medical Research AUS, using mRNA from adult N. americanus (Shangai strain) maintained in Hamsters.

characteristic: description

text: Adult

text: Necator americanus

text: Mixed

text: Shanghai

text: Necator americanus adult cDNA library


text: Institute of Marine Research

text: live

text: Generic

text: cDNA library constructed from pooled thymus samples of Atlantic halibut (Hippoglossus hippoglossus L) injected with IPN Virus

text: Hippoglossus hippoglossus

characteristic: organism

text: Thymus

text: cDNA library from thymus cells of Hippoglossus hippoglossus injected with IPN Virus TEB

text: pBluescript II


text: Institute of Marine Research

text: live

text: Generic

text: cDNA library constructed from pooled thymus samples of Atlantic halibut (Hippoglossus hippoglossus L) injected with Nodavirus

text: Hippoglossus hippoglossus

text: Thymus

text: cDNA library from thymus cells of Hippoglossus hippoglossus injected with Nodavirus TEC

text: pBluescript II


text: Institute of Marine Research

text: live

text: Generic

text: cDNA subtraction library from Atlantic halibut

text: Hippoglossus hippoglossus

text: ECI Hippoglossus hippoglossus subtraction library

text: pBluescript II


text: Cummings School of Veterinary Medicine at Tufts University

text: live

text: Generic

text: most cell types

characteristic: cell type

text: Whole shrimp (cephalotorax and tail) from a specific pathogen-free shrimp from a low-surviving (4.3%) family cross after challenge per os with TSV was used for total RNA isolation. The CLONTECH PCR-SMARTTM cDNA kit was used for library construction.

text: juvenile

text: E. coli (ElectroMAX DH5a-E

text: whole juvenile shrimp

text: Penaeus vannamei

text: Sfi A (5'end)

text: Sfi B (3'end)

text: most tissue types

text: pDNR-LIB (CLONETECH)


text: Hefei Teachers College

text: live

text: Generic

text: Phytophthora sojae

text: phytophthora sojae oosporogenesis express library


text: Genoscope - Centre National de Sequencage

text: live

text: Generic

text: genotype: B97 C-C-2; young cushions

text: Theobroma cacao

text: Cushion

characteristic: tissue-type


text: live

text: Generic

text: genotype: Scavina6; Pod tissue infected by Phytophthora palmivora

text: Theobroma cacao

text: Pod


text: live

text: Generic

text: genotype: Scavina6; cortex tissue, external part

text: Theobroma cacao

text: Cortex


text: live

text: Generic

text: genotype: Scavina6; Flowers at different stages of development

text: Theobroma cacao

text: Flower


text: live

text: Generic

text: genotype: Scavina6; cherels from 1 week to 1 month stage of development

text: Theobroma cacao

text: Cherel


text: live

text: Generic

text: genotype: Scavina6; SSH library from ovaries submitted to compatible/incompatible pollinations

text: Theobroma cacao

text: Ovary


text: live

text: Generic

text: genotype: ICS1AF; hypocotyle from 2-3 week germinated seeds

text: Theobroma cacao

text: Hypocotyle


text: live

text: Generic

text: genotype: Jaca; stem tissues inoculated by Ceratocystis fimbriata

text: Theobroma cacao

text: Stem


text: live

text: Generic

text: genotype: Scavina6; leaves submited to drought stresses

text: Theobroma cacao

text: Leave


text: live

text: Generic

text: genotype: Scavina6; cortex tissue internal part with lignified chanels

text: Theobroma cacao

text: Cortex


text: live

text: Generic

text: genotype: Scavina6; roots submited to drought stresses

text: Theobroma cacao

text: Root


text: live

text: Generic

text: genotype: PNG seedlings; leaves inoculated by Phytophthora palmivora

text: Theobroma cacao

text: Leave


text: live

text: Generic

text: genotype: Scavina6; young and adult leaves at diferent stages of development

text: Theobroma cacao

text: Leave


text: live

text: Generic

text: genotype: UPA134; pod tissues infected by Phytophthora megakarya

text: Theobroma cacao

text: Pod


text: live

text: Generic

text: genotype: UF676; young shoot tissues attacked by Sahlbergella singularis (mirids)

text: Theobroma cacao

text: Shoot


text: live

text: Generic

text: genotype: PNG seedlings; SSH library from infected leaves by Phytophthora megakarya from resistant - suceptible PNG seedlings

text: Theobroma cacao

text: Leave


text: live

text: Generic

text: genotype: Scavina6; pod tissues infected by Moniliophthora perniciosa less than 60 days after inoculation

text: Theobroma cacao

text: Pod


text: Genoscope - Centre National de Sequencage

text: live

text: Generic

text: genotype: Scavina6; roots

text: Theobroma cacao

text: Root


text: live

text: Generic

text: genotype: P7; pod tissues infected by Moniliophthora roreri

text: Theobroma cacao

text: Pod


text: live

text: Generic

text: genotype: ICS1; seeds 3 to 3,5 months after pollinations

text: Theobroma cacao

text: Seed


text: live

text: Generic

text: genotype: PNG seedlings; SSH library from infected leaves by Phytophthora megakarya from suceptible-resistant PNG seedlings

text: Theobroma cacao

text: Leave


text: live

text: Generic

text: genotype: IMC47; Ovaries from 1 to 7 days after pollinations

text: Theobroma cacao

text: Ovule


text: live

text: Generic

text: genotype: Scavina6; SSH library from infected-non infected pod tissues by Moniliophthora perniciosa between 60 to 120 days after inoculation

text: Theobroma cacao

text: Pod


text: live

text: Generic

text: genotype: ICS1; ovules collected 2 to 3 months after pollination

text: Theobroma cacao

text: Ovule


text: live

text: Generic

text: genotype: BE240; seeds 2 to 5 months after pollinations

text: Theobroma cacao

text: Seed


text: live

text: Generic

text: genotype: Scavina6; pod tissues infected by Moniliophthora perniciosa between 60 to 120 days after inoculation

text: Theobroma cacao

text: Pod


text: live

text: Generic

text: genotype: ICS1; SSH library from tissues infected/non infected by Phytophthora palmivora

text: Theobroma cacao

text: Cortex


text: live

text: Generic

text: genotype: GU255V; leaves inected by Phytophthora palmivora

text: Theobroma cacao

text: Leave


text: live

text: Generic

text: genotype: PNG seedlings; SSH library from non infected- infected leaves of resistant seedlings, by Phytophthora palmivora

text: Theobroma cacao

text: Leave


text: live

text: Generic

text: genotype: Scavina6; SSH library from tissues infected/non infected by Phytophthora palmivora

text: Theobroma cacao

text: Cortex


text: live

text: Generic

text: genotype: ICS1; cotyledons from germinated seeds (1 to 3 weeks)

text: Theobroma cacao

text: Cotyledon


text: live

text: Generic

text: genotype: ICS1; seeds 4 to 5 months after pollinations

text: Theobroma cacao

text: Seed


text: live

text: Generic

text: genotype: 33-49; cotyledons from seeds fermented between 6H and 4 days

text: Theobroma cacao

text: Seed


text: live

text: Generic

text: genotype: ICS1AF; epicotyle from 2-3 week germinated seeds

text: Theobroma cacao

text: Seed


text: Genoscope - Centre National de Sequencage

text: live

text: Generic

text: genotype: ICS1AF; epicotyle and hypocotyle from 1 week germinated seeds

text: Theobroma cacao

text: Seed


text: live

text: Generic

text: genotype: UF273; pod tissues infected by Monilia roreri

text: Theobroma cacao

text: Pod


text: live

text: Generic

text: genotype: PNG seedlings; SSH library from infected-non infected leaves of resistant seedlings, by Phytophthora megakarya

text: Theobroma cacao

text: Leave


text: live

text: Generic

text: genotype: ICS1; seeds from mature pods 5,5 to 6 months after pollinations

text: Theobroma cacao

text: Seed


text: live

text: Generic

text: genotype: Scavina6; SSH library from infected-non infected pod tissues by Moniliophthora perniciosa less than 60 days after inoculation

text: Theobroma cacao

text: Pod


text: live

text: Generic

text: genotype: PNG seedlings; SSH library from infected-non infected leaves of resistant seedlings, by Phytophthora palmivora

text: Theobroma cacao

text: Leave


text: live

text: Generic

text: genotype: PNG seedlings; SSH library from infected leaves by Phytophthora palmivora from resistant - suceptible PNG seedlings

text: Theobroma cacao

text: Leave


text: Molecular Biology and Biotechnology, NRC on Plant Biotechnology

text: live

text: Generic

text: Nagina 22

characteristic: cultivar

text: collected_by: Sangram Keshari Lenka; collection_date: 25-Aug-2007; country: India

text: Oryza sativa Indica Group

text: whole plant

text: Oryza sativa Indica Group Nagina 22 whole plant


text: National Institute of Biomedical Innovation

text: live

text: Generic

text: 1st strand cDNA was primed with an oligo(dT) primer [ATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded cDNA was synthesized using specific 5' and 3' primers and amplified by PCR. The PCR product was digested with SfiI and size selection was performed to exclude fragments <1.5kb.The SfiI-digested PCR product was cloned into distinct DraIII sites of pME18S-FL3. XhoI sites just outside the DraIII sites can be used to isolate the cDNA insert. Libraries were constructed by oligo-capping method.

text: 23 years old

characteristic: dev-stage

text: Pan troglodytes verus

text: DraIII (CACTGTGTG)

text: DraIII (CACCATGTG)

text: male

characteristic: sex

text: testis

text: chimpanzee testis cDNA library PtsC

characteristic: title

text: pME18S-FL3 (Acc.No. AB009864)


characteristic: External Id

text: North Carolina A&T State University

text: live

text: Generic

text: blood neutrophil

text: Breed: Holstein Friesian

text: Bos taurus

text: Female

text: Bos taurus blood neutrophil


text: North Carolina A&T State University

text: live

text: Generic

text: peripheral blood mononuclear cells

text: Breed: Boxer

text: Canis lupus familiaris

text: Female

text: Canis lupus familiaris peripheral blood mononuclear cells


text: University of Wuerzburg

text: live

text: Generic

text: cDNA library was created using the Creator SMART cDNA Library Construction Kit

text: Milnesium tardigradum

text: Plasmid

text: pDNR-LIB

characteristic: vector


text: Florida A & M University

characteristic: INSDC center name

text: live

text: Generic

text: Blue Lake

text: Used Differential Display RT-PCR to isolated mRNA induced specifically upon Elsinoe ampelina infestation from leaf tissue.

text: Leaf

characteristic: organ

text: Vitis hybrid cultivar

text: Leaf tissue

text: Elsinoe ampelina induced genes


text: University of Bern

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Laboratory strain. Rosetting rate N/A. Ring stage parasites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Ring

characteristic: isolate

text: Human

text: Plasmodium falciparum

text: Blood

text: Laboratory isolate 3D7AH1 Pf Ring stage var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Ring

text: Human

text: Plasmodium falciparum

text: Blood

text: Laboratory isolate 3D7AH1S.2 Pf Ring stage var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Laboratory strain. Rosetting rate N/A. Trophozoite stage parasites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

characteristic: description

text: Trophozoite

text: Human

characteristic: lab-host

text: Plasmodium falciparum

characteristic: organism

text: Blood

text: Laboratory isolate 3D7AH1S.2 Pf Trophozoite stage var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Trophozoite

text: Human

text: Plasmodium falciparum

text: Blood

characteristic: tissue-type

text: Laboratory isolate 3D7AH1 Pf Trophozoite stage var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Ring

characteristic: dev-stage

text: Human

text: Plasmodium falciparum

text: Blood

text: Laboratory isolate 7G8 Pf Ring stage var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Trophozoite

text: Human

text: Plasmodium falciparum

text: Blood

text: Laboratory isolate 7G8 Pf Trophozoite stage var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Laboratory strain. Rosetting rate 30%. Ring stage parasites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Ring

text: Human

text: Plasmodium falciparum

text: Blood

text: Laboratory isolate DD2 Pf Ring stage var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Laboratory strain. Rosetting rate 30%. Trophozoite stage parasites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Human

text: Plasmodium falciparum

text: Blood

text: Laboratory isolate DD2 Pf Trophozoite stage var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Ring

text: Human

text: Plasmodium falciparum

text: Blood

text: Laboratory isolate FCR3 Pf Ring stage var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Trophozoite

text: Human

text: Plasmodium falciparum

text: Blood

text: Laboratory isolate FCR3 Pf Trophozoite stage var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Laboratory strain. Rosetting rate 5%. Ring stage parasites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Ring

text: Human

text: Plasmodium falciparum

text: Blood

text: Laboratory isolate FCR3s1.6 Pf Ring stage var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Laboratory strain. Rosetting rate 5%. Trophozoite stage parasites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Human

text: Plasmodium falciparum

text: Blood

text: Laboratory isolate FCR3s1.6 Pf Trophozoite stage var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Laboratory strain. Rosetting rate 31%. Ring stage parasites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Ring

text: Human

text: Plasmodium falciparum

text: Blood

text: Laboratory isolate TM284 Pf Ring stage var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Laboratory strain. Rosetting rate 31%. Trophozoite stage parasites were enriched, and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Human

text: Plasmodium falciparum

text: Blood

text: Laboratory isolate TM284 Pf Trophozoite stage var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAM52b from Apac, Uganda originated from a patient sufferring from mild malaria. Erythrocytes were separated from other blood constituents and frozen down in sorbitol glycerol solution. The parasites were thawed with NaCl and cultivated for 11 generations. The parasites were enriched in ring stage and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Ring

text: Human

text: Plasmodium falciparum

text: Blood

text: In vitro adapted parasite UAM52b Pf Ring stage var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAM52b from Apac, Uganda originated from a patient sufferring from mild malaria. Erythrocytes were separated from other blood constituents and frozen down in sorbitol glycerol solution. The parasites were thawed with NaCl and cultivated for 11 generations. The parasites were enriched in mature trophozoite stage and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Human

text: Plasmodium falciparum

text: Blood

text: In vitro adapted parasite UAM52b Pf Trophozoite stage var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAS22b from Apac, Uganda originated from a patient sufferring from severe malaria. Erythrocytes were separated from other blood constituents and frozen down in sorbitol glycerol solution. The parasites were thawed with NaCl and cultivated for 11 generations. The parasites were enriched in ring stage and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Ring

text: Human

text: Plasmodium falciparum

text: Blood

text: In vitro adapted parasite UAS22b Pf Ring stage var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAS22b from Apac, Uganda originated from a patient sufferring from severe malaria. Erythrocytes were separated from other blood constituents and frozen down in sorbitol glycerol solution. The parasites were thawed with NaCl and cultivated for 11 generations. The parasites were enriched in mature trophozoite stage and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Human

text: Plasmodium falciparum

text: Blood

text: In vitro adapted parasite UAS22b Pf Trophozoite stage var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAS29b from Apac, Uganda originated from a patient sufferring from severe malaria. Erythrocytes were separated from other blood constituents and frozen down in sorbitol glycerol solution. The parasites were thawed with NaCl and cultivated for 11 generations. The parasites were enriched in ring stage and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Ring

text: Human

text: Plasmodium falciparum

text: Blood

text: In vitro adapted parasite UAS29b Pf Ring stage var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAS29b from Apac, Uganda originated from a patient sufferring from severe malaria. Erythrocytes were separated from other blood constituents and frozen down in sorbitol glycerol solution. The parasites were thawed with NaCl and cultivated for 11 generations. The parasites were enriched in mature trophozoite stage and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Human

text: Plasmodium falciparum

text: Blood

text: In vitro adapted parasite UAS29b Pf Trophozoite stage var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAS31b from Apac, Uganda originated from a patient sufferring from severe malaria. Erythrocytes were separated from other blood constituents and frozen down in sorbitol glycerol solution. The parasites were thawed with NaCl and cultivated for 11 generations. The parasites were enriched in ring stage and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Ring

text: Human

text: Plasmodium falciparum

text: Blood

text: In vitro adapted parasite UAS31b Pf Ring stage var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAS31b from Apac, Uganda originated from a patient sufferring from severe malaria. Erythrocytes were separated from other blood constituents and frozen down in sorbitol glycerol solution. The parasites were thawed with NaCl and cultivated for 11 generations. The parasites were enriched in mature trophozoite stage and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Human

text: Plasmodium falciparum

text: Blood

text: In vitro adapted parasite UAS31b Pf Trophozoite stage var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAS41b from Apac, Uganda originated from a patient sufferring from severe malaria. Erythrocytes were separated from other blood constituents and frozen down in sorbitol glycerol solution. The parasites were thawed with NaCl and cultivated for 11 generations. The parasites were enriched in ring stage and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Ring

text: Human

text: Plasmodium falciparum

text: Blood

text: In vitro adapted parasite UAS41b Pf Ring stage var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UAS41b from Apac, Uganda originated from a patient sufferring from severe malaria. Erythrocytes were separated from other blood constituents and frozen down in sorbitol glycerol solution. The parasites were thawed with NaCl and cultivated for 11 generations. The parasites were enriched in mature trophozoite stage and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Human

text: Plasmodium falciparum

text: Blood

text: In vitro adapted parasite UAS41b Pf Trophozoite stage var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UKM19 from Kampala, Uganda originated from a patient sufferring from mild malaria. Erythrocytes were separated from other blood constituents, parasites enriched and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Ring

text: Human

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UKM19 Pf Ring stage var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UKM19 from Kampala, Uganda originated from a patient sufferring from mild malaria. Erythrocytes were separated from other blood constituents and cultured until parasites matured into trophozoites. Trophozoites were enriched and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Human

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UKM19 Pf Trophozoite stage var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UKM64 from Kampala, Uganda originated from a patient sufferring from mild malaria. Erythrocytes were separated from other blood constituents, parasites enriched and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Ring

text: Human

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UKM64 Pf Ring stage var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UKM64 from Kampala, Uganda originated from a patient sufferring from mild malaria. Erythrocytes were separated from other blood constituents and cultured until parasites matured into trophozoites. Trophozoites were enriched and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Human

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UKM64 Pf Trophozoite stage var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UKS14 from Kampala, Uganda originated from a patient sufferring from severe malaria. Erythrocytes were separated from other blood constituents, parasites enriched and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Ring

text: Human

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UKS14 Pf Ring stage var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UKS14 from Kampala, Uganda originated from a patient sufferring from severe malaria. Erythrocytes were separated from other blood constituents and cultured until parasites matured into trophozoites. Trophozoites were enriched and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Human

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UKS14 Pf Trophozoite stage var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UKS15 from Kampala, Uganda originated from a patient sufferring from severe malaria. Erythrocytes were separated from other blood constituents, parasites enriched and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Ring

text: Human

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UKS15 Pf Ring stage var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UKS25 from Kampala, Uganda originated from a patient sufferring from severe malaria. Erythrocytes were separated from other blood constituents, parasites enriched and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Ring

text: Human

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UKS25 Pf Ring stage var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UKS3 from Kampala, Uganda originated from a patient sufferring from severe malaria. Erythrocytes were separated from other blood constituents, parasites enriched and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Ring

text: Human

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UKS3 Pf Ring stage var gene cDNA

text: pcrII-TOPO