text: Incyte Genomics

text: live

text: Generic

text: Homo sapiens

text: Human cDNA expression libraries


status: PUBLIC

characteristic: External Id

text: North Carolina State University

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Trichoplusia ni Hubner

characteristic: description

text: 5th stadium, day 1 and 2

tag: attribute

characteristic: dev-stage

text: Gut

characteristic: organ

text: Trichoplusia ni

characteristic: organism

text: EcoRI

text: XhoI

text: Shorey, University of California at Riverside

characteristic: strain

text: Trichoplusia ni Lambda Zap Express cDNA Library

characteristic: title

text: Phagemid

text: Lambda Zap

characteristic: vector


text: University of California, Berkeley

text: live

text: Generic

text: cDNA made by oligo-dT priming. Library constructed by Dr. Francesca Mariani in the laboratory of R. Harland, Ph.D. (University of California, Berkeley). References: XBF-2 is a transcriptional repressor that converts ectoderm into neural tissue. Mariani, FV. Harland, RM., Development. 1998 Dec;125(24):5019-31. PMID: 9811586; UI: 99030283; Use of large-scale expression cloning screens in the xenopus laevis tadpole to identify gene function. Grammer TC. Liu KJ. Mariani FV. Harland RM,. Dev Biol. 2000 Dec 15;228(2):197-210.

text: stage 19-23

text: Xenopus laevis

text: neurula

characteristic: tissue-type

text: Harland stage 19-23 Xenopus laevis cDNA

text: plasmid


text: Stowers Institute for Medical Research

text: live

text: Generic

text: hematopoietic cells

characteristic: cell type

text: Adult

text: Mus musculus

text: female

characteristic: sex

text: hematopoietic tissues

text: psub expressed cDNA library


characteristic: External Id

text: Stowers Institute for Medical Research

text: live

text: Generic

text: hematopoietic cells

text: Adult

text: Mus musculus

text: female

text: hematopoietic tissues

text: Lambda ZAP expressed cDNA library


text: GuangZhou Medical College

characteristic: INSDC center name

text: live

text: Generic

text: Bronchial epithelial cell

characteristic: cell-line

text: cDNA sequence from Homo sapiens 16HBE cells transformed by BPDE

text: Homo sapiens

text: Human transformed 16HBE cDNA

text: Plasmid

text: pGEM-T


characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Oryza sativa

text: Oryza sativa agrochemical-induced transcript


text: University of Edinburgh

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: The infective L1 larva of Trichinella spiralis is a nematode parasite of mammalian skeletal muscle. The library was constructed using muscle larvae of the T. spiralis isolate ISS3, and was provided by Dr Bernadette Connolly, University of Aberdeen.

text: muscle stage larvae

text: Trichinella spiralis

text: SalI (5'end)

text: NotI (3'end)

text: mixed

text: Trichinella spiralis muscle stage larvae (BC)

text: Phage

text: Lambda ZipLox


text: University of Edinburgh

text: live

text: Generic

characteristic: NCBI submission package

text: The infective L1 larva of Trichinella spiralis is a nematode parasite of mammalian skeletal muscle. The library was constructed using muscle larvae of the T. spiralis isolate ISS115, and was provided by Dr Teresa Garate, Instituto de Salud Carlos III

characteristic: description

text: muscle stage larvae

text: Trichinella spiralis

text: EcoRI (5'end)

text: XhoI (3'end)

text: mixed

text: Trichinella spiralis muscle stage larvae (TG)

text: Phage

text: Lambda Zap II


text: Monsanto

text: live

text: Generic

text: Monsanto bovine skeletal muscle EST sequences

text: adult

characteristic: dev-stage

text: Bos taurus

text: female

text: skeletal muscle

text: Monsanto bovine skeletal muscle cDNA library BOVMS1


text: MIPS

text: live

text: Generic

text: adult

characteristic: lab-host

text: Homo sapiens

characteristic: organism

text: NotI

text: SalI

text: human spinal cord

text: 451 (synonym: hlcc1) spinal cord


text: MIPS

text: live

text: Generic

text: adult

text: Homo sapiens

text: NotI

text: SalI

text: human skeletal muscle

text: 451 (synonym: hlcc1) skeletal muscle

characteristic: title


text: Ecole Normale Superieure, INSERM 412

text: live

text: Generic

text: country=United Kingdom

text: Gallus gallus

text: Gallus gallus library (Castellazzi MA)


text: Henan Medical University

text: live

text: Generic

text: Human esophageal carcinoma subtracted cDNA library.

text: adult

text: Homo sapiens

text: male

text: esophagus carcinoma tissue


text: Institut de Biologie de Lille, Institut Pasteur de Lille

text: live

text: Generic

text: RDA (representational difference analysis) of 5 days old pup kidneys from VEGF120/120 knock out mice versus wild type mice.

text: 5 days old pups

text: Kidney

characteristic: organ

text: Mus musculus

text: male and female

text: 129/Sv x Swiss

characteristic: strain

text: plasmid

text: pBluescript SK

characteristic: vector


text: West Virginia University

text: live

text: Generic

text: Smooth muscle

characteristic: cell type

text: Intestine

text: Oncorhynchus mykiss

text: EcorR I

text: Xho I

text: Rainbow Trout Intestine Lambda Zap II Vector library

text: Plasmid

text: pBluescript SK(+/-)


characteristic: External Id

text: Henan Medical University

text: live

text: Generic

text: cDNA library constracted using mRNA differential display PCR technique

text: Homo sapiens

text: esophagus carcinoma tissue

characteristic: tissue-type

text: Human esophageal carcinoma cDNA library


text: Washington University School of Medicine

characteristic: INSDC center name

text: live

text: Generic

text: Construction of the cDNA library was performed by Dr. W. Gregg Clark using a modification of the cDNA synthesis protocol developed in the laboratory of Dr. Michael Lovett by Dr. Yulia Korshunova (personal communication). First polyA + RNA was isolated from total gametophore RNA using oligo dT magnetic beads. Following this, first strand cDNA synthesis was performed on the bead-bound polyA + RNA, during which an oligonucleotide anchor sequence was incorporated onto the 5'-ends of the cDNA. PCR amplification was then used to synthesize the second strand, to amplify the double stranded DNA, and to incorporate dUTP containing sequences into the ends of the double stranded cDNA. This DNA was size selected and cloned into pAMP1 using the CloneAMP pAMP1 System (Life Technologies, GibcoBRL) for cloning amplification products by a non-restriction site dependant process. The cloning was directional based on sequence asymmetry introduced at the ends during PCR amplification. The 3' cDNA ends are proximal to the NotI site of the multiple cloning site in pAMP1. This annealing mixture was transformed into chemically competent DH10B cells and selected for ampicillin resistant growth. The resulting clones (about 330,000) were pooled to make the library.

text: Physcomitrium patens

text: gametophore: 30 day old tissue, ammonium-grown

text: Moss EST library PPG


characteristic: INSDC first public

text: live

text: Generic

text: Construction of this library was carried out using Stratagene's Lambda ZAP II-cDNA synthesis kit. cDNA was synthesized using an oligo dT primer and made blunt-ended. EcoRI adapters then were ligated to the blunt ends, excess adapters were removed, and the cDNA was ligated to Lambda ZAP II arms. The vector is designed containing the pBluescript sequence as well as lambda DNA and cDNA is cloned within this pBluescript sequence. The vector was then packaged using Gold Gigapackaging extracts. Library was grown in XLIBlue MRF' cells and amplified. The library was excised by mass excision using Stratagene's 'Mass excision kit' that uses exassist as a helper phage that releases the pBluescript sequence and circularises it as single strand plasmids that are then packaged (by helper phage) and secreted out of the host cell as phagemids. SOLR cells were transformed with phagemids and the library was plated out on LB-amp plates to select for transformants. Approximately 500,000 colonies were plated and pooled for isolation of the double stranded plasmid DNA library. This DNA was used to transform DH10B cells, the clones from which were used as a source of DNA for the sequencing project. This library was constructed by Dr. Stavros Bashiardes as part of the Physcomitrella EST program (PEP) at the University of Leeds (UK) and Washington University in St. Louis (USA). DNA sequencing by Washington University Genome Sequencing Center. For information on obtaining a clone please contact: Celia Knight (c.d.knight@leeds.ac.uk).

text: Physcomitrium patens

text: EcoRI

text: EcoRI

text: protonemata 7 day old, abscisic acid treated

text: Moss EST library PPA

text: phagemid

text: pBluescript SK-


text: Clemson University

characteristic: INSDC last update

text: live

text: Generic

text: Morex

characteristic: cultivar

text: Plants were grown in the greenhouse at the University of California, Riverside (Fenton, SJ Close, TJ Close). Whole spikes with awns trimmed were collected at 20 DAP (Fenton). Total RNA was prepared, poly(A) RNA was purified, one primary unamplified cDNA library was made, and 1 million pfu were in vivo excised to give pBluescript SK(-) cDNA phagemids in the TJ Close lab at the University of California, Riverside (Choi). Phagemids were plated and picked at the Clemson University Genomics Institute (CUGI) (Begum, Palmer, Frisch, Atkins and Wing). Plasmid DNA preparations, DNA sequencing and sequence analysis were performed at CUGI (Wing, Yu, Frisch, Henry, Simmons, Oates, Rambo, Main). The sequence has been trimmed to remove vector sequence and contains a minimum of 100 bases of phred value 20 or above. For more details on library preparation and sequence analysis see http://www.genome.clemson.edu/projects/barley. To order this clone see http://www.genome.clemson.edu/orders Also see Close TJ, Wing R, Kleinhofs A, Wise R (2001) Genetically and physically anchored EST resources for barley genomics. Barley Genetics Newsletter 31:29-30. (http://wheat.pw.usda.gov/ggpages/bgn/31/cover.html)

text: SOLR

text: Hordeum vulgare subsp. vulgare

text: 20 DAP spike

text: Hordeum vulgare 20 DAP spike EST library HVcDNA0010 (20 DAP)

text: Phage

text: lambdaZAP


text: Monsanto Company

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: 5 DAP

text: Zea mays

text: primary shoot, 2 cm

text: Corn 5DAP Immature Shoot Library

text: Plasmid

text: pSPORT


text: USDA-ARS

text: live

text: Generic

characteristic: NCBI submission package

text: Isolation and cloning of differential display products

characteristic: description

text: Mature Leaf

text: leaf

text: Glycine max

text: leaf

text: Plasmid

text: pGEM-T Easy Vector.


text: Children's Memorial Research Center

text: live

text: Generic

text: The UI-R-CS0 library is a normalized library constructed from the following rat heart tissues: embryonic day 17, embryonic day 19, embryonic day 21, adult day 1, adult day 12, adult day 75, adult day 200. For a detailed description of the library from which this clone was derived, please visit our web site at ratest.eng.uiowa.edu. The subtraction has been previously described in (Bonaldo, Lennon and Soares, Genome Research 6:791-806, 1996)

text: ADULT

characteristic: dev-stage

text: DH10B (Life Technologies)

characteristic: lab-host

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid


text: Children's Memorial Research Center

text: live

text: Generic

text: The UI-R-CS0s library is a non-normalized library constructed from the following rat heart tissues: embryonic day 17, embryonic day 19, embryonic day 21, adult day 1, adult day 12, adult day 75, adult day 200. For a detailed description of the library from which this clone was derived, please visit our web site at ratest.eng.uiowa.edu. The subtraction has been previously described in (Bonaldo, Lennon and Soares, Genome Research 6:791-806, 1996)

text: ADULT

text: DH10B (Life Technologies)

text: Rattus norvegicus

characteristic: organism

text: Not I

text: Eco RI

text: Sprague-Dawley

characteristic: title

text: Phagemid


text: Children's Memorial Research Center

text: live

text: Generic

text: The UI-R-CT0 library is a normalized library constructed from the following rat brain tissues: embryonic day 17, embryonic day 19, embryonic day 21, adult day 1, adult day 12, adult day 75, adult day 200. For a detailed description of the library from which this clone was derived, please visit our web site at ratest.eng.uiowa.edu. The subtraction has been previously described in (Bonaldo, Lennon and Soares, Genome Research 6:791-806, 1996)

text: ADULT

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

characteristic: strain

text: Phagemid


text: Children's Memorial Research Center

text: live

text: Generic

text: The UI-R-CT0s library is a non-normalized library constructed from the following rat brain tissues: embryonic day 17, embryonic day 19, embryonic day 21, adult day 1, adult day 12, adult day 75, adult day 200. For a detailed description of the library from which this clone was derived, please visit our web site at ratest.eng.uiowa.edu. The subtraction has been previously described in (Bonaldo, Lennon and Soares, Genome Research 6:791-806, 1996)

text: ADULT

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid

characteristic: vector


text: Children's Memorial Research Center

text: live

text: Generic

text: The UI-R-CU0 library is a normalized library constructed from the following rat kidney tissues: embryonic day 17, embryonic day 19, embryonic day 21, adult day 1, adult day 12, adult day 75, adult day 200. For a detailed description of the library from which this clone was derived, please visit our web site at ratest.eng.uiowa.edu. The subtraction has been previously described in (Bonaldo, Lennon and Soares, Genome Research 6:791-806, 1996)

text: ADULT

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid


text: Children's Memorial Research Center

text: live

text: Generic

text: The UI-R-CU0s library is a non-normalized library constructed from the following rat kidney tissues: embryonic day 17, embryonic day 19, embryonic day 21, adult day 1, adult day 12, adult day 75, adult day 200. For a detailed description of the library from which this clone was derived, please visit our web site at ratest.eng.uiowa.edu. The subtraction has been previously described in (Bonaldo, Lennon and Soares, Genome Research 6:791-806, 1996)

text: ADULT

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid


text: Children's Memorial Research Center

text: live

text: Generic

text: The UI-R-CV0 library is a non-normalized library constructed from rat eye tissue. For a detailed description of the library from which this clone was derived, please visit our web site at ratest.eng.uiowa.edu. The subtraction has been previously described in (Bonaldo, Lennon and Soares, Genome Research 6:791-806, 1996)

text: ADULT

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid


text: Children's Memorial Research Center

text: live

text: Generic

text: The UI-R-CV1 library is a normalized library constructed from rat eye tissue. For a detailed description of the library from which this clone was derived, please visit our web site at ratest.eng.uiowa.edu. The subtraction has been previously described in (Bonaldo, Lennon and Soares, Genome Research 6:791-806, 1996)

text: ADULT

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid


text: Children's Memorial Research Center

text: live

text: Generic

text: The UI-R-CW0 library is a normalized library constructed from the following rat aorta tissues: embryonic day 19, embryonic day 21, adult day 1, adult day 12, adult day 75, adult day 200. For a detailed description of the library from which this clone was derived, please visit our web site at ratest.eng.uiowa.edu. The subtraction has been previously described in (Bonaldo, Lennon and Soares, Genome Research 6:791-806, 1996)

text: ADULT

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid


text: Children's Memorial Research Center

text: live

text: Generic

text: The UI-R-CW0s library is a non-normalized library constructed from the following rat aorta tissues: embryonic day 19, embryonic day 21, adult day 1, adult day 12, adult day 75, adult day 200. For a detailed description of the library from which this clone was derived, please visit our web site at ratest.eng.uiowa.edu. The subtraction has been previously described in (Bonaldo, Lennon and Soares, Genome Research 6:791-806, 1996)

text: ADULT

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid


text: Children's Memorial Research Center

text: live

text: Generic

text: The UI-R-CX0 library is a normalized library constructed from the following rat placenta tissues: embryonic day 17, embryonic day 19, embryonic day 21. For a detailed description of the library from which this clone was derived, please visit our web site at ratest.eng.uiowa.edu. The subtraction has been previously described in (Bonaldo, Lennon and Soares, Genome Research 6:791-806, 1996)

text: ADULT

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid


text: Children's Memorial Research Center

text: live

text: Generic

text: The UI-R-CX0s library is a non-normalized library constructed from the following rat placenta tissues: embryonic day 17, embryonic day 19, embryonic day 21. For a detailed description of the library from which this clone was derived, please visit our web site at ratest.eng.uiowa.edu. The subtraction has been previously described in (Bonaldo, Lennon and Soares, Genome Research 6:791-806, 1996)

text: ADULT

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid


text: Children's Memorial Research Center

text: live

text: Generic

text: The UI-R-CY0 library is a non-normalized library constructed from rat brown adipose tissue. For a detailed description of the library from which this clone was derived, please visit our web site at ratest.eng.uiowa.edu. The subtraction has been previously described in (Bonaldo, Lennon and Soares, Genome Research 6:791-806, 1996)

text: ADULT

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid


text: Children's Memorial Research Center

text: live

text: Generic

text: The UI-R-CZ0 library is a non-normalized library constructed from rat penis tissue. For a detailed description of the library from which this clone was derived, please visit our web site at ratest.eng.uiowa.edu. The subtraction has been previously described in (Bonaldo, Lennon and Soares, Genome Research 6:791-806, 1996)

text: ADULT

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid


text: Children's Memorial Research Center

text: live

text: Generic

text: The UI-R-DA0 library is a non-normalized library constructed from rat salivary gland tissue. For a detailed description of the library from which this clone was derived, please visit our web site at ratest.eng.uiowa.edu. The subtraction has been previously described in (Bonaldo, Lennon and Soares, Genome Research 6:791-806, 1996)

text: ADULT

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid


text: Children's Memorial Research Center

text: live

text: Generic

text: The UI-R-DB0 library is a non-normalized library constructed from rat bladder tissue. For a detailed description of the library from which this clone was derived, please visit our web site at ratest.eng.uiowa.edu. The subtraction has been previously described in (Bonaldo, Lennon and Soares, Genome Research 6:791-806, 1996)

text: ADULT

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid


text: Children's Memorial Research Center

text: live

text: Generic

text: The UI-R-DC0 library is a non-normalized library constructed from rat seminal vesicle tissue. For a detailed description of the library from which this clone was derived, please visit our web site at ratest.eng.uiowa.edu. The subtraction has been previously described in (Bonaldo, Lennon and Soares, Genome Research 6:791-806, 1996)

text: ADULT

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid


text: Children's Memorial Research Center

text: live

text: Generic

text: The UI-R-DD0 library is a non-normalized library constructed from rat fundus region of the stomach: the upper region around the oesophageal spinchter and stratified-type epithelium. For a detailed description of the library from which this clone was derived, please visit our web site at ratest.eng.uiowa.edu. The subtraction has been previously described in (Bonaldo, Lennon and Soares, Genome Research 6:791-806, 1996)

text: ADULT

text: DH10B (Life Technologies)

text: fundus region of the stomach

characteristic: organ

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: fundus region of the stomach

text: Phagemid


characteristic: External Id

text: Children's Memorial Research Center

text: live

text: Generic

text: The UI-R-DE0 library is a non-normalized library constructed from rat cervix tissue. For a detailed description of the library from which this clone was derived, please visit our web site at ratest.eng.uiowa.edu. The subtraction has been previously described in (Bonaldo, Lennon and Soares, Genome Research 6:791-806, 1996)

text: ADULT

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid


text: Beijing Institute of Heart Lung and Blood Vessels Disease

text: live

text: Generic

text: Rattus norvegicus

text: Sprague-Dawley

text: myocardium

characteristic: tissue-type

text: rat myocardium

text: T- Easy Vector


text: Vanderbilt University

characteristic: INSDC center name

text: live

text: Generic

text: Pancreatic endocrine tumor

characteristic: cell type

characteristic: cell-line

text: mRNA from GKP2 and GKP4 cells was used to perform subtraction suppression hybridization. Genes that are more highly expressed in GKP2 cells (an insulinoma derived cell), compared to GKP4 cells (a precursor cell line) were cloned pCR 2.1-TOPO and transformed into TOP10 competent bacteria.

text: TOP10 (Invitrogen, Carlsbad, CA)

text: Pancreas

text: Mus musculus

text: C57BL/6J x DBA/2

text: GKP2 subtraction cDNA library

text: Plasmid

text: pCR 2.1-TOPO (Invitrogen, Carlsbad, CA)


text: Vanderbilt University

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Pancreatic endocrine tumor

text: mRNA from GKP2 and GKP4 cells was used to perform subtraction suppression hybridization. Genes that are more highly expressed in GKP4 cells (a precursor cell line), compared to GKP2 cells (an insulinoma derived cell) were cloned pCR 2.1-TOPO and transformed into TOP10 competent bacteria.

text: TOP10 (Invitrogen, Carlsbad, CA)

text: Pancreas

text: Mus musculus

text: C57BL/6J x DBA/2

text: GKP4 subtraction cDNA library

text: Plasmid

text: pCR 2.1-TOPO (Invitrogen, Carlsbad, CA)


text: The Rockefeller University

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Weinstein,D.C., Honore,E., and Hemmati-Brivanlou,A. (1997). Epidermal induction and inhibition of neural fate by translation initiation factor 4AIII. Development 124, 4235-4242.

text: 10 - 10.5

text: Xenopus laevis

text: Sal I

text: Not I

text: Stage 10+ Gastrula Library

text: Plasmid


text: Basic Medicine Reseach Institute, Beijing University of Chinese Traditional Medicine

text: live

text: Generic

characteristic: NCBI submission package

text: Hippocampus

text: Rattus norvegicus

text: male

characteristic: sex

text: Sprague-Dawley

text: rat Hippocampus

text: TEasy vector


text: Clemson University

text: live

text: Generic

text: Morex

characteristic: cultivar

text: Plants were raised from seeds in a Controlled Environments growth chamber maintained in continuous light at 18oC, and testa and pericarp were dissected from developing kernels at Washington State University, Pullman, WA (Kannangara, von Wetstein). Total RNA was prepared, poly(A) RNA was purified, one cDNA library was made, and 1 million pfu were in vivo excised to give pBluescript SK(-) cDNA phagemids in the TJ Close lab at the University of California, Riverside (Akhunov, Chin, Choi, Close, Fenton, Kianian, Otto, Simons, Zhang). Phagemids were plated and picked at the Clemson University Genomics Institute (CUGI) (Begum, Palmer, Frisch, Atkins and Wing). Plasmid DNA preparations, DNA sequencing and sequence analysis were performed at CUGI (Wing, Yu, Frisch, Henry, Simmons, Oates, Rambo, Main). The sequence has been trimmed to remove vector sequence and contains a minimum of 100 bases of phred value 20 or above. For more details on library preparation and sequence analysis see http://www.genome.clemson.edu/projects/barley. To order this clone see http://www.genome.clemson.edu/orders Also see Close TJ, Wing R, Kleinhofs A, Wise R (2001) Genetically and physically anchored EST resources for barley genomics. Barley Genetics Newsletter 31:29-30. (http://wheat.pw.usda.gov/ggpages/bgn/31/cover.html)

characteristic: description

characteristic: lab-host

text: Hordeum vulgare subsp. vulgare

text: testa/pericarp

text: Hordeum vulgare testa/pericarp EST library HVcDNA0013 (normal)

text: Phage

text: lambdaZAP


text: Henan Normal University

text: live

text: Generic

text: embryonic day 17 post-fertilization

characteristic: dev-stage

text: Rattus norvegicus

characteristic: organism

text: male

text: Sprague-Dawley

characteristic: strain

text: liver

text: Rat Liver Express Library

characteristic: title


characteristic: External Id

text: University of Edinburgh

text: live

text: Generic

text: Necator americanus is a human hookworm, responsible for debilitating anaemia. The library was constructed by David Pritchard (University of Nottingham, UK) from mRNA from N.americanus L3 stage (Nottingham strain) maintained in hamsters.

text: Necator americanus

text: EcoRI (5'end)

text: XhoI (3'end)

text: Mixed

text: Necator americanus (parasitic nematode) L3

text: Plasmid

text: pCMV-PCR vector

characteristic: vector


text: University of Edinburgh

characteristic: INSDC center name

text: live

text: Generic

text: Necator americanus is a human hookworm, responsible for debilitating anaemia. The library was constructed by David Pritchard (University of Nottingham, UK) from mRNA from N.americanus L4 stage (Nottingham strain) maintained in hamsters.

text: Necator americanus

text: EcoRI (5'end)

text: XhoI (3'end)

text: Mixed

text: Necator americanus (parasitic nematode) L4

text: Plasmid

text: pCMV-PCR vector


text: Institute of Agricultural Science and Technology(NIAST)

text: live

text: Generic

text: ASI 2029

text: average insert size:1500 bp; initial pfu:5 * 10 7; Isolation of total RNA from the mature fruiting body cultivated in poplar tree sawdust bottle

text: mature fruiting body

text: E.coli

text: Pleurotus ostreatus

text: SfiIA

text: SfiIBI

text: lambda TripEX2


text: Peking University

text: live

text: Generic

text: Homo sapiens

text: brain

characteristic: tissue-type

text: Homo sapiens brain (H Wang)


text: National Institute for Basic Biology

characteristic: INSDC first public

text: live

text: Generic

text: adult

text: Oryzias latipes

text: HNI

text: whole body

text: Sugano-Kawakami 5' end enriched cDNA library (OLa) from HNI


text: National Institute for Basic Biology

characteristic: INSDC last update

text: live

text: Generic

text: Oryzias latipes

text: HNI

text: UV irradiated OLHNI cell line cDNA library (OLc)


text: National Institute for Basic Biology

text: live

characteristic: INSDC status

text: Generic

text: adult

text: Oryzias latipes

text: HNI

text: ovary

text: Medaka ovary cDNA library (OLd) from HNI


text: National Institute for Basic Biology

text: live

text: Generic

characteristic: NCBI submission model

text: adult

text: Oryzias latipes

text: HNI

text: liver

text: Medaka liver cDNA library (OLe) from HNI


text: Graduate School of Science, Nagoya University

text: live

text: Generic

characteristic: NCBI submission package

text: Wild samples from Okayama Pref.(Southern part of Japan)

text: adult

text: Oryzias latipes

text: wild type

text: eye

text: Medaka eye cDNA library (SNK01)


text: University of Ulsan

text: live

text: Generic

text: 60-day old

text: Brain

characteristic: organ

text: Rattus norvegicus

text: female

characteristic: sex

text: Sprague-Dawley

text: Hypothalamus

text: Rat mRNA identified by differential display PCR


text: live

text: Generic

text: Random genomic sequence

characteristic: description

text: Saccharomyces cariocanus

text: UFRJ 50791

text: Saccharomyces cariocanus UFRJ 50791

text: Phage


text: live

text: Generic

text: Random genomic sequence

text: Saccharomyces mikatae

text: IFO 1815

text: Saccharomyces mikatae IFO 1815

text: Phage


text: Universidade do Algarve

text: live

text: Generic

text: adult

text: E. coli

characteristic: lab-host

text: Fucus vesiculosus

text: male, female

text: thallus

text: cDNA suppression subtraction library

text: plasmid

text: pGEM-T Easy


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: cDNA made by oligo-dT priming. Directionally cloned into EcoRI/XhoI sites using the following 5' adaptor: GGCACGAG(G). Library constructed by Ling Hong in the laboratory of Gerald M. Rubin (University of California, Berkeley) using ZAP-cDNA synthesis kit (Stratagene) and Superscript II RT (Life Technologies). Note: this is a NIH_MGC Library.

text: DH10B (phage-resistant)

text: skin

text: Homo sapiens

text: XhoI

text: EcoRI

text: amelanotic melanoma, cell line

text: plasmid (chloramphenicol resistant, grown at 30C)


text: Institution of Medical Biology, CAMS

text: live

text: Generic

text: fibroblast

characteristic: cell type

text: Fibroblast cDNA library induced by binding of HAV

text: lung

text: Homo sapiens

text: FIBHAVI


text: SLU

text: live

text: Generic

text: germination 7-days after sowing

characteristic: dev-stage

text: Euphorbia lagascae

characteristic: organism

text: cotyledon and endosperm

text: Euphorbia lagascae pBluescript XR cDNA library


text: Central South University

text: live

text: Generic

text: Epithelium

text: While cDNA from human adult nasopharynx was used as tester and complex cDNA from trachea, bronchia, oesophagus, lung, liver, heart, spleen, skeletal muscle, kidney and skin as driver, the differentially expressed products of human nasopharynx were obtained by suppression subtractive hybridization .

text: 25 years old

text: Homo sapiens

text: male

text: Nasopharynx

text: Human nasopharynx subtractive cDNA Library

characteristic: title

text: Plasmid

text: pGEM-T Easy


characteristic: External Id

text: Indian Institute of Horticultural Research

text: live

text: Generic

text: Alphonso

characteristic: cultivar

text: It is a cDNA product (obtained by RT-PCR) which was differentially expressed when amplified using two non-specific primers

text: Middle ripe stage

text: Fruit

text: Mangifera indica

text: Xba I

text: Bam H I

text: Hermaphrodite

text: Pulp

text: Mango fruit cv. Alphonso middle ripe stage

text: Plasmid

text: PUC 57


text: Washington University School of Medicine

characteristic: INSDC center name

text: live

text: Generic

text: The cDNA library wms constructed from mRNA isolated from 2-3 week old seedlings that were induced for symptoms of SDS (Sudden Death Syndrome) disease by the translocation of culture filtrate of Fusarium solani f. sp. glycines (Plant Cell Report 18:375-380). Cultivar PI 567374 is partially resistant to the disease SDS. Plant tissue (expanded leaves, folded leaves, and new shoots) were collected at 1, 6, 24, and 48 hrs. after inoculation and their mRNA pooled equally for cDNA construction. The library was prepared using the Stratagene pBluescript II SK(+) library construction kit. Complementary DNA was synthesized from mRNA using a primer consisting of a poly(dT) sequence with an XhoI restriction site. EcoRI adaptors were ligated to the blunt-ended cDNA fragments followed by XhoI digestion. The cDNA insert is protected from XhoI digestion via methylation during first strand synthesis. The cDNA fragments were directionally cloned into the EcoRI-XhoI restriction site of the pBluescript vector. The ligated cDNA fragments were transformed into E.coli ElectroMax DH10B host cells. Plants were inoculated by Shuxian Li (Glen Hartman lab, University of Illinois). Library was constructed by Steve Clough (Lila Vodkin lab, University of Illinois).

text: 2-3 weeks old

text: Glycine max

text: EcoRI

text: XhoI

text: seedlings induced for symptoms of SDS (Sudden Death Syndrome) disease

text: plasmid

text: pBluescript II SK+

characteristic: vector


characteristic: INSDC first public

text: live

text: Generic

text: Williams 82

text: The cDNA library was constructed from mRNA isolated from 2-3 week old seedlings that were induced for symptoms of SDS (Sudden Death Syndrome) disease by the translocation of culture filtrate of Fusarium solani f. sp. glycines (Plant Cell Report 18:375-380). Cultivar Williams 82 is susceptible to the disease SDS. Plant tissue (expanded leaves, folded leaves, and new shoots) were collected at 1, 6, 24, and 48 hrs. after inoculation and their mRNA pooled equally for cDNA construction. The library was prepared using the Stratagene pBluescript II SK(+) library construction kit. Complementary DNA was synthesized from mRNA using a primer consisting of a poly(dT) sequence with an XhoI restriction site. EcoRI adaptors were ligated to the blunt-ended cDNA fragments followed by XhoI digestion. The cDNA insert is protected from XhoI digestion via methylation during first strand synthesis. The cDNA fragments were directionally cloned into the EcoRI-XhoI restriction site of the pBluescript vector. The ligated cDNA fragments were transformed into E.coli ElectroMax DH10B host cells. Plants were inoculated by Shuxian Li (Glen Hartman lab, University of Illinois). Library was constructed by Reena Philip and Steve Clough (Lila Vodkin lab, University of Illinois).

text: 2-3 weeks old

text: Glycine max

text: EcoRI

text: XhoI

characteristic: tissue-type

text: plasmid

text: pBluescript II SK+


characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Williams 82

text: The cDNA library was constructed from mRNA isolated from 9-11 day old seedlings that were induced for HR (hypersensitive response) by vacuum infiltrating plant tissue with Pseudomonas syringae pv. glycinea carrying the avrB gene (Genetics 141:1597-1604). Plant tissue (expanded unifoliate leaves) was collected at 2, 4, 8, 12, 24, 36, and 53 hrs after inoculation and their mRNA pooled equally for cDNA construction. The library was prepared using the Stratagene pBluescript II SK(+) library construction kit. Complementary DNA was synthesized from mRNA using a primer consisting of a poly(dT) sequence with an XhoI restriction site. EcoRI adaptors were ligated to the blunt-ended cDNA fragments followed by XhoI digestion. The cDNA insert is protected from XhoI digestion via methylation during first strand synthesis. The cDNA fragments were directionally cloned into the EcoRI-XhoI restriction site of the pBluescript vector. The ligated cDNA fragments were transformed into E.coli ElectroMax DH10B host cells. Plant care, inoculations, and library construction were performed by Steve Clough (Lila Vodkin lab, University of Illinois).

text: 9-11 day old

text: Glycine max

text: EcoRI

text: XhoI

text: seedlings induced for HR (hypersensitive response)

text: plasmid

text: pBluescript II SK+


text: Tianjin Cancer Institute Hospital, Tianjin Medical University

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Clinical Stage I

text: Breast

text: Homo sapiens

text: Female

text: Breast cancer

text: Human Genome Express Library


text: Istituto Nazionale Tumori

text: live

text: Generic

characteristic: cell-line

text: a unidirectional, oligo(dT) primed cDNA library was cloned into the lambda-ZAP express vector starting from poly(A)+ RNA. cDNA fragments were size selected in a range of 0.5-10 kb and inserted into the ZAP Express vector (Stratagene) in the sense orientation with respect to the lacZ promoter.

text: adult

text: breast

characteristic: organ

text: Homo sapiens

text: EcoRI

text: XhoI

text: female

characteristic: sex

text: tumor

text: SKBR3 Lambda Zap Express Library (ATCC#HTB-30)

text: Bacteriophage

text: lambda ZAP Express II


text: live

text: Generic

text: Anchor enzyme: NlaIII~ Granular cell precursor cells from normal, 8-day old mice were purified by enzymatic digestion and percoll gradient centrifugation, pelleted and snap-frozen. Library constructed in the laboratory of G. Riggins, M.D., Ph.D. (Duke University).

characteristic: description

characteristic: lab-host

text: brain

text: Mus musculus

text: granular cell precursor cells

text: plasmid


text: Institute of Botany, CAS

text: live

text: Generic

text: isolated by cold acclimation differential display; authority: Cucumis sativus L Jinyan No:4

text: seedling with two true leaves

characteristic: dev-stage

text: Cucumis sativus

characteristic: organism

text: leaf

text: Cucumis sativus seedling with two true leaves


characteristic: External Id

text: Agriculture and Agri-Food Canada - SCPFRC

text: live

text: Generic

text: Landrace

text: This cDNA library was constructed from polyA+ enriched mRNA from field grown leaves. Mid-size actively growing leaves were collected and pooled from several plants and frozen immediately after harvesting in liquid nitrogen. The cDNA was prepared using an XhoI-poly(dT) linker-primer. An EcoRI adapter was ligated to the blunt end cDNA and the products were digested with EcoRI and XhoI enabling directional cloning into the lambda ZAP Express vector. The library was amplified using the host strain XL1-Blue MRF'. Mass excision of the library was performed to obtain pBK-CMV phagemid clones in the host strain XLOLR. Single pass DNA sequencing was performed using the T3 promoter primer: 5' ATTAACCCTCACTAAAGGGA 3'. This library was constructed by Alex Richman.

text: field grown, mid-size

text: E. coli strain XLOLR

text: Stevia rebaudiana

text: EcoRI

text: XhoI

characteristic: strain

text: leaf

text: Stevia field grown leaf cDNA

characteristic: title

text: ds phagemid

text: pBK-CMV


text: Sumitomo Electric Industries

characteristic: INSDC center name

text: live

text: Generic

text: fetal

text: Homo sapiens

text: liver

text: Homo sapiens liver fetal (Kishimoto,T.)


text: Friedrich Miescher-Institut

characteristic: INSDC first public

text: live

text: Generic

text: Bintje

characteristic: cultivar

text: infected with Phytophthora infestans

text: Solanum tuberosum

text: leaf

text: Solanum tuberosum cv. Bintje leaf


text: Ghent University, Faculty of Veterinary Medicine

characteristic: INSDC last update

text: live

text: Generic

text: Ostertagia ostertagi

text: Ostertagia ostertagi library


text: Gyeongsang National University

text: live

characteristic: INSDC status

text: Generic

text: Jangwon

text: XL-1 Blue

text: Brassica rapa subsp. pekinensis

text: Sal I

text: Not I

text: Etiolated seedling

characteristic: tissue-type

text: Chinese cabbage etiolated seedling library

text: Plasmid

text: pSPORT 1

characteristic: vector


text: University of Bologna

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: country=Italy

text: adult

text: Sus scrofa

text: skeletal muscle, biceps femoris

text: Pig skeletal muscle, phagemid vector pT7T3

text: phagemid


text: Otto-von-Guericke University Magdeburg

text: live

text: Generic

text: lymphocytes

characteristic: cell type

characteristic: cell-line

text: Differential display comparison of untreated and dimethylfumarate treated U937 cells

text: Homo sapiens

text: Dimethylfumarate induced differential expression of mRNA in U937 cells


text: Kyowa Hakko Kogyo

text: live

text: Generic

text: epithelial

text: HBdl12, derived from primary kidney epithelial cells

text: PCR-representational difference analysis was applied to mRNAs from two cell lines derived from primary neonatal rat kidney by immortalization with a wild type adenovirus E1A 12S cDNA or a mutant HBdl12, wherein the mutant derived cell line was the tester and the wild type the driver. Therefore the cDNAs activated or upregulated in the mutant were the targets. Differential expression was validated by RT-PCR and Northern analyses of the cDNAS obtained on the source mRNAs. The WT derived cells retained epithelial and mature kidney specific expression profiles. The HBdl12 derived cells have lost many such characteristics and have undergone epithelial mesenchymal transition (EMT) and have many characteristics of metanephric mesenchyme. The two cell types respond differently when exposed to V12ras.

characteristic: description

text: neonatal

text: E. coli XL1-Blue

characteristic: lab-host

text: kidney

characteristic: organ

text: Rattus norvegicus

text: M13 forward

text: M13 reverse

text: Fisher F344

text: Rat kidney: upregulated in EMT and tumor progression

text: plasmid

text: pBluescript II SK(-)


characteristic: External Id

text: Shanghai Institute of Biochemistry and Cell Biology

text: live

text: Generic

text: Homo sapiens

characteristic: organism

text: human uterine leiomyoma subtractive library


text: Institut Pasteur

text: live

text: Generic

text: selected by DDRT-PCR from unfed and human blood-fed mosquito

text: adult

characteristic: dev-stage

text: Anopheles gambiae

characteristic: strain

text: midgut

text: Anopheles gambiae midgut G3 adult

characteristic: title


text: Institut Pasteur

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: selected by DDRT-PCR on Plasmodium falciparum-infected mosquito

text: adult

text: Anopheles gambiae

text: Yaounde

text: midgut

text: Anopheles gambiae midgut Yaounde adult


text: Institute of Medical Science, University of Tokyo

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Homo sapiens

text: Sugano Homo sapiens cDNA library


text: live

text: Generic

text: Anchor enzyme: NlaIII~ Primary tumor: well-differentiated fibrillary astrocytoma derived from a 50 year old female. Library constructed in the laboratory of G. Riggins, M.D., Ph.D. (Duke University).

text: brain

text: Homo sapiens

text: female

characteristic: sex

text: primary tumor

characteristic: tissue-type

text: plasmid

characteristic: vector


text: Washington University School of Medicine

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Williams 82

characteristic: cultivar

text: The cDNA library was constructed from mRNA isolated from 11 day old seedlings treated with that were treated with 2 ugs/ml of a crude glucan elicitor preparation isolated from the mycelial walls of Phytophthora sojae. The library was prepared using the Stratagene pBluescript II SK(+) library construction kit. Complementary DNA was synthesized from mRNA using a primer consisting of a poly(dT) sequence with an XhoI restriction site. EcoRI adaptors were ligated to the blunt-ended cDNA fragments followed by XhoI digestion. The cDNA fragments were directionally cloned into the EcoRI-XhoI restriction site of the pBluescript vector. The ligated cDNA fragments were transformed into E.coli ElectroMax DH10B host cells. Plant material was provided by Michael G. Hahn (Complex Carbohydrate Research Center, University of Georgia) and the library was constructed by Anu Khanna (Lila Vodkin lab, University of Illinois).

text: 11 day old seedlings

text: Glycine max

text: EcoRI

text: XhoI

text: wounded cotyledons

text: plasmid

text: pBluescript II SK+


text: live

text: Generic

text: Jack

text: The cDNA library was constructed from mRNA isolated from differentiating somatic embryos cultered on MSM6AC. The library was prepared using the Stratagene pBluescript II SK(+) library construction kit. Complementary DNA was synthesized from mRNA using a primer consisting of a poly(dT) sequence with an XhoI restriction site. EcoRI adaptors were ligated to the blunt-ended cDNA fragments followed by XhoI digestion. The cDNA fragments were directionally cloned into the EcoRI-XhoI restriction site of the pBluescript vector. The ligated cDNA fragments were transformed into E.coli ElectroMax DH10B host cells. Tissue culture and library construction were performed by Franooise Thibaud-Nissen and Anu Khana (Lila Vodkin lab, University of Illinois).

characteristic: description

characteristic: lab-host

text: Glycine max

text: EcoRI

text: XhoI

text: plasmid

text: pBluescript II SK+


characteristic: External Id

text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: cDNAs were oligo-dT primed and directionally cloned. Staging according to Nieuwkoop and Faber. Library was constructed by N. Garrett, P. LeMaire, A.M. Zorn, and J.B. Gurdon (Wellcome/CRC Institute).

text: DH10B (phage-resistant)

text: Xenopus laevis

text: NotI

text: EcoRI

text: embryo, stage 10.5

text: Wellcome CRC pRN3 St10 5

text: plasmid (ampicillin resistant)


text: Vanderbilt Medical Center

text: live

text: Generic

text: Colon cancer cell

characteristic: cell type

characteristic: cell-line

text: Poly A RNA was isolated from NS-398 treated and control cells, and suppression subtractive hybridization was performed in forward and reverse directions. The adapter sequences used in the hybridization were as follows: 5'-CTAATACGACTCACTATAGGGCTCGAGCGGCCGCCCGGGCAGGT-3' (adapter 1) and 5'-CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGT-3' (adapter 2R).

text: Homo sapiens

characteristic: organism

text: SSH-HCA-D library

text: Plasmid


text: Vanderbilt Medical Center

text: live

text: Generic

text: Colon cancer cell

text: Homo sapiens

text: SSH-HCA-U library

characteristic: title

text: Plasmid


text: Max-Planck-Institute for Plant Breeding Research

characteristic: INSDC center name

text: live

text: Generic

text: cDNA was ligated to a 15-mer oligonucleotide adaptor (Stratagene) and cloned into the EcoRI site of the Bluescript plasmid vector

text: 4 week old pot-grown plants

characteristic: dev-stage

text: shoot and root

characteristic: organ

text: Beta vulgaris

text: MPIZ-ADIS 006 Lambda Zap II library

text: Plasmid

text: Bluescript


text: Capital Institute of Medicine, Anzhen Hospital

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: lymphocytes

text: Homo sapiens

text: Human lymphocytes


text: live

text: Generic

text: cDNAs were oligo-dT primed and directionally cloned. Staging according to Nieuwkoop and Faber. Library was constructed by N. Garrett, E. Bellefroid, and A.M. Zorn, (Wellcome/CRC Institute).

text: DH10B (phage-resistant)

text: Xenopus laevis

text: NotI

text: EcoRI

text: pooled embryos, stage 13-17

characteristic: tissue-type

text: Wellcome CRC pRN3 St13 17

text: plasmid (ampicillin resistant)

characteristic: vector


text: The Institute for Genomic Research (TIGR; www.tigr.org)

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Kennebec

text: Whole plants were challenged with 450,000 sporangia/ml P. infestans US-1(US 940501) in Biotron (Madison, Wisconsin). Leaf tissue was collected at 1, 2, 5, 12, and 24 hours post-challenge and frozen in liquid nitrogen immediately upon removal. Kennebec plants showed no signs of HR. Katahdin plants (susceptible to P. infestans US-1) were used as controls and showed infection. NOTE: We cannot exclude the possibility that this sequence is actually derived from Phytophthora rather than potato.

text: 6 week old

text: SOLR

text: Solanum tuberosum

text: EcoRI

text: XhoI

text: leaf

text: P. infestans-challenged leaf

text: pBluescript SK(-)


text: live

text: Generic

text: Kennebec

characteristic: cultivar

text: Various sizes of sprouting eyes (2mm to 15mm) were taken from tubers. The tubers were incubated at 26C in the dark for 2-3 weeks prior to sprouting. The eyes were frozen in liquid nitrogen immediately upon removal from tubers.

characteristic: description

text: 12-14 weeks post harvest

text: SOLR

text: Solanum tuberosum

text: EcoRI

text: XhoI

text: sprouting eyes from tubers

text: cSTS

text: pBluescript SK(-)


characteristic: External Id

text: The Institute of Molecular Genetics, Russian Academy of Sciences

text: live

text: Generic

text: B-lymphocytes

text: The cDNA library was resulted from the subtractive hybridization of RNA from centroblastic lymphoma cells (tracer) and RNA from immunoblastic lymphoma cells (driver)

text: adult

text: left tonsil

text: Homo sapiens

text: Apa I

text: EcoR I

text: male

characteristic: sex

text: Human HIV associated B-cell centroblastic lymphoma

text: Plasmid

text: pBluescript SK


text: School of Basic Medical Sciences, Ji Lin University

text: live

text: Generic

text: mononuclear cell

characteristic: cell type

text: authority=Cervus nippon Temminck

text: Cervus nippon

text: male

text: blood

text: Deer blood cDNA library


text: Institute of Basic Medical Sciences, Peking Union Medical College & Chinese Academy of Medical Sciences

text: live

text: Generic

text: B lymphocyte

text: Homo sapiens

characteristic: organism

text: tonsils

text: human activated B lymphocyte


text: The Institute for Genomic Research

text: live

text: Generic

text: TA cloning; Plasmodium yoelii sporozoite cDNA library from salivary gland sporozoites 14 days post-infection

text: sporozoites from salivary gland

text: E. coli TOP10

characteristic: lab-host

text: Plasmodium yoelii

characteristic: strain

text: Plasmodium yoelii sporozoite cDNA

characteristic: title

text: plasmid


text: US Department of Agriculture, Agriculture Research Service, Pacific West Area, Western Regional Research Center

characteristic: INSDC center name

text: live

text: Generic

text: The tissue, total RNA, and poly(A) RNA were prepared from apex at double-ridge stage to terminal-spikelet stage during transition from vegetative state to flower state, a cDNA library was made, and the cDNA clones were in vivo excised at the University of California, Davis (V. Echenique, B. Stamova, J. Dubcovsky). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

text: Seven week-old plants

characteristic: dev-stage

text: E. coli XLOLR

text: Triticum monococcum

text: EcoRI

text: XhoI

text: Early reproductive apex

text: Triticum monococcum early reproductive apex cDNA library

text: Phagemid

text: Lambda Uni-ZAP XR, excised phagemid


characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised at the University of California, Davis (V. Echenique, B. Stamova, J. Dubcovsky). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

text: Three weeks-old plants

text: E. coli XLOLR

text: Triticum monococcum

text: EcoRI

text: XhoI

text: Vegetative shoot apex

text: Triticum monococcum vegetative apex cDNA library

text: Phagemid

text: Lambda pBK-CMV (Lambda Zap Express), excised phagemid


text: Ji Lin University, School of Basic Medical Sciences

text: live

characteristic: INSDC status

text: Generic

text: authority=Cervus nippon Temminck

text: Cervus nippon

text: female

text: aorta

characteristic: tissue-type

text: deer aorta cDNA library


text: live

text: Generic

characteristic: NCBI submission model

text: authority=Cervus nippon Temminck

text: Cervus nippon

text: female

text: aorta

text: deer aorta cDNA PCR library


text: King Faisal Specialist Hospital and Research Centre

text: live

text: Generic

characteristic: NCBI submission package

text: Epithelial

characteristic: cell-line

text: Breast

characteristic: organ

text: Homo sapiens

text: Female

text: Normal mammary gland

text: Breast Cells Differential Display ARE-cDNA PCR

text: Plasmid

characteristic: vector


characteristic: External Id

text: live

text: Generic

text: monocyte

text: THP-1 monocytic cell line

text: Homo sapiens

text: acute monocytic leukemia

text: THP-1 Differential Display ARE-cDNA PCR

text: Plasmid

text: pCR 2.1


text: live

text: Generic

text: Homo sapiens

text: Normal Kidney

text: Kidney Cells Differential Display ARE-cDNA PCR

text: Plasmid

text: pCR 2.1


text: live

text: Generic

text: Monocytic

characteristic: cell type

text: Homo sapiens

text: Normal Adherent

text: Monocytes Differential Display ARE-cDNA PCR

text: Plasmid

text: pCR 2.1


text: Roslin Institute

text: live

text: Generic

text: Sus scrofa

characteristic: organism

text: adipose

text: Sus scrofa adipose cDNA


text: live

text: Generic

text: Brevor

characteristic: cultivar

text: Plants were grown to seed maturity under conditions favoring seed dormancy (L. Dohery at K. Walker_Simmons lab, Washington State University, Pullman, WA). Embryos were cut from mature dormant seed (Doherty). Total RNA was prepared from these embryos, polyA was purified, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab at the University of California, Riverside (Chin, Fenton). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

characteristic: description

text: Mature seed

text: E. coli SOLR

characteristic: lab-host

text: Triticum aestivum

text: EcoRI

text: XhoI

text: Seed embryo

text: Wheat dormant embryo cDNA library

text: Phagemid

text: Lambda Uni-ZAP XR, excised phagemid


text: Medical University of South Carolina

characteristic: INSDC center name

text: live

text: Generic

text: Crassostrea virginica

text: Sfi I

text: Sfi I

text: Embryo, D-veliger larvae

text: CvEMB

characteristic: title

text: Plasmid


text: Medical University of South Carolina

characteristic: INSDC first public

text: live

text: Generic

text: Crassostrea virginica

text: Sfi I

text: Sfi I

text: Hemocyte

text: CvHEM

text: Plasmid


text: University of Delaware

characteristic: INSDC last update

text: live

text: Generic

text: E.coli EMDH10B

text: Gallus gallus

text: Male and Female

characteristic: sex

text: thymus, bursa, spleen, PBL, bone marrow

text: Normalized chicken lymphoid cDNA library

text: pCMVSPORT 6


text: University of Kuopio

text: live

characteristic: INSDC status

text: Generic

text: adult

characteristic: dev-stage

text: Rattus norvegicus

text: EcoRI

text: XhoI

text: Wistar

characteristic: strain

text: brain entorhinal cortex

characteristic: tissue-type

text: Rattus norvegicus brain entorhinal cortex PC12 cell line

text: pBluescript (Stratagene)


text: Cornell University

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Flowers and flower buds were collected from greenhouse grown plants and used for library construction (cLEL).

text: 4-8 week old plants

text: XLOLR

text: Solanum lycopersicum

text: EcoRI

text: XhoI

text: developing flower buds and open flowers

text: Tomato flower library from a mixture of developmental stages


characteristic: External Id

text: University of Delaware

text: live

text: Generic

text: E.coli EMDH10B

text: Gallus gallus

text: Male and Female

text: liver

text: Chicken liver cDNA library

text: pCMVSPORT 6

characteristic: vector


text: Cornell University

text: live

text: Generic

text: reproductive

characteristic: cell type

text: Dissected accessory glands

text: adult

text: Drosophila simulans

characteristic: organism

text: male

text: accessory gland

text: Drosophila accessory gland pSport1 library


text: The First Military Medical University

text: live

text: Generic

text: Total RNA was isolated from hypothalamus and transcripted into cDNA, which was then used as templat in PCR.The PCR products were separated on sequencing gel. The differential bands were cut, reamplified, cloned into pMD18-T vector and confirmed by Northern blot.

characteristic: description

text: 2 months

text: Rattus norvegicus

text: Sprague-Dawley

text: Hypothalamus

text: differentially expressed gene from hypothalamus of heat acclimated rats


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: cDNA made by oligo-dT priming. Directionally cloned into EcoRI/XhoI sites using the following 5' adaptor: GGCACGAG(G). Library constructed by Ling Hong in the laboratory of Gerald M. Rubin (University of California, Berkeley) using ZAP-cDNA synthesis kit (Stratagene) and Superscript II RT (Life Technologies). Note: this is a NIH_MGC Library.

text: DH10B (phage-resistant)

characteristic: lab-host

text: prostate

characteristic: organ

text: Homo sapiens

text: XhoI

text: EcoRI

text: carcinoma, cell line

text: plasmid (chloramphenicol resistant, grown at 30C)


text: Sars International Centre for Molecular Marine Biology

characteristic: INSDC center name

text: live

text: Generic

text: This directional library has been made using Stratagene's ZAP Express cDNA Synthesis Kit by Drs. Fabio Spada and Eric Thompson at the Sars Int'l Centre, Thormohlensgt. 55, N-5008 Bergen, Norway.

text: adult (4-day)

text: XL1-Blue MRF', XLOLR strains

text: whole animal

text: Oikopleura dioica

text: EcoRI

text: XhoI

text: mixed

text: Oikopleura dioica ZAP Express Library

characteristic: title

text: pBK-CMV


text: National University of Singapore

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: rhabdomyosarcoma (RD) cell line

characteristic: cell-line

text: The cDNA library was prepared using the anchor primer, H-T11G, H-T11A & H-T11C from Genhunter

text: embryo

text: Homo sapiens

text: female

text: rhabdomyosarcoma

text: Human rhabdomyosarcoma cDNA Library


text: School of Basic Medical Sciences, Ji Lin University

text: live

characteristic: INSDC status

text: Generic

text: authority=Cervus nippon Temminck

text: Cervus nippon

text: male

characteristic: sex

text: spleen medulla

text: Deer spleen cDNA library


text: University of Georgia

text: live

text: Generic

characteristic: cultivar

text: cDNA was prepared from polyA+ enriched RNA. The cDNA was directionally ligated into the Unizap XR vector from Stratagene and packaged using Gigapack III Gold packaging extracts. Plasmids containing cDNA inserts were excised from the recombinant lambda-Zap phage using Ex-assist helper phage and propagated in SOLR cells.

text: 24 hours after treatment in the dark at 26 C with 0.5 mg/ml oligogalacturonides (DP 6-20) in the presence of 100 ug/ml Gentamicin

characteristic: dev-stage

text: XLOLR

text: Medicago truncatula

text: EcoRI

text: XhoI

text: 3 day old seedling roots

characteristic: tissue-type

text: HOGA

text: phagemid

text: pBluescript SK-


characteristic: External Id

text: College of Medicine, Yonsei University

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Entamoeba histolytica

characteristic: strain

text: Entamoeba histolytica YS-27


text: Harvard University, Howard Hughes Medical Institute

text: live

text: Generic

text: cDNA made by oligo-dT priming. Size-selected on agarose gel. Average insert size ~1kb. 5' XhoI site was destroyed after directional cloning. Amplified once. Contact information: Hiroshi Inoue, MD, Metabolism Div. (Alan Permutt Lab), Washington University School of Medicine, Box 8127, 660 South Euclid Ave., St. Louis, MO 63110, E-mail: hinoue@imgate.wustl.edu, Tel: 314-362-1916, Fax: 314-747-2692.

characteristic: description

text: Pancreas

text: Homo sapiens

characteristic: organism

text: NotI

text: XhoI

text: Purified pancreatic islet

text: HR85 islet

text: plasmid (ampicillin resistant)

text: pBluescript SK(-)

characteristic: vector


text: Washington University School of Medicine

characteristic: INSDC center name

text: live

text: Generic

text: This library was constructed by Keliang Tang, Robert Cole, and L. David Sibley at Washington University. cDNAs were synthesized from poly(A)+ RNA by olidod(T) priming, size-selected and directionally cloned into the Uni-ZAP XR lambda vector (Stratagene). The primary library was mass excised as phagemids and rescued in SOLR cells. The plasmid library was recovered from the SOLR cells and transformed in mass into DH10B (GeneHog, Research Genetics, Inc.) for sequencing. WARNING: This library may contain a small percentage contaminants from human fibroblast cells.

text: Tachyzoite

characteristic: lab-host

text: Toxoplasma gondii

text: EcoRI

text: XhoI

text: VEG

text: TgVEG118 Tachyzoite cDNA Library

text: pBluescript SK


text: Institute of Infectious Diseases of P.L.A., The 302 Hospital of P.L.A.

characteristic: INSDC first public

text: live

text: Generic

text: 24hrs after liver partial cutoff

text: E. coli DH5alpha

text: liver

characteristic: organ

text: Rattus norvegicus

text: male

text: Rat Library of liver regeneration

characteristic: title

text: Plasmid

text: pGEM-T easy


text: National Institute of Animal Industry

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Day 28 pig embryo

text: Sus scrofa

text: lambda ZAP pig embryo


text: live

text: Generic

text: SL1-Oligo(dT) PCR-based library. Parastrongyloides trichosuri cDNA PCR products of size >400 nucleotides containing SL1 on the 5' end and oligo(dT) on the 3' end were non-directionally cloned into pCRII-TOPO (Invitrogen) following the Topo TA cloning protocol. The cDNA insert can be excised by digestion with EcoRI. The library was constructed by Brandi Chiapelli and Dr. James McCarter at Washington University, St. Louis.

text: Infective larvae

text: Parastrongyloides trichosuri

text: Parastrongyloides trichosuri IL SL1 TOPO v2

text: plasmid (amp. or kan. resistant)

text: pCRII-TOPO


text: Institute of Neuroscience

text: live

text: Generic

text: adult

text: Rattus norvegicus

text: male

characteristic: sex

text: Sprague-Dawley

text: dorsal root ganglion

text: Rat DRG Library


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.5kb. Library constructed by Life Technologies. Note: this is a NCI_CGAP Library.

text: DH10B (T1 phage-resistant)

text: skin

text: Homo sapiens

text: NotI

text: SalI

text: phagemid (ampicillin resistant)


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: DH10B (T1 phage-resistant)

text: skin

text: Homo sapiens

text: NotI

text: SalI

text: squamous cell carcinoma

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: cDNA made by oligo-dT priming. Directionally cloned into EcoRI/XhoI sites using the following 5' adaptor: GGCACGAG(G). Size-selected >500bp for average insert size 1.8kb. Library constructed by Ling Hong in the laboratory of Gerald M. Rubin (University of California, Berkeley) using ZAP-cDNA synthesis kit (Stratagene) and Superscript II RT (Life Technologies). Note: this is a NIH_MGC Library.

text: DH10B (phage-resistant)

text: brain

text: Homo sapiens

text: XhoI

text: EcoRI

text: neuroblastoma, cell line


text: Universita degli Studi d ella Basilicata

text: live

text: Generic

text: Library constructed by M.J.T. Van EIJK of the Netherlands Institute of Developmental Biology (Utrecht); country=Netherlands

text: Bos taurus

text: placenta

text: Bos taurus placenta (M.J.T. Van EIJK)


text: Miyazaki University

text: live

text: Generic

text: common name:common carp ; stimulated by lipo-polysaccharide and concanavalin-A

text: Cyprinus carpio

text: head kidney

text: Cyprinus carpio head kidney stimulated by lipo-polysaccharide and concanavalin-A


text: Basis Medical School of Jilin University

text: live

text: Generic

text: adult

characteristic: dev-stage

text: Cervus nippon

text: Not I

text: Sal I

text: male

text: Shuangyang sika deer

text: spleen cells

text: Shuangyang sika deer plasmid cDNA library

text: pSPORT


text: live

text: Generic

text: adult

text: Cervus nippon

text: male

text: Shuangyang sika deer

text: pilose

characteristic: tissue-type

text: Shuangyang sika deer pilose lambda gt10 cDNA library

text: lambda gt10


characteristic: External Id

text: live

text: Generic

text: adult

text: Cervus nippon

text: male

text: Shuangyang sika deer

text: pilose

text: Shuangyang sika deer pilose PCR cDNA library

text: TA vector


text: live

text: Generic

characteristic: NCBI submission model

text: adult

text: Cervus nippon

text: male

text: Shuangyang sika deer

text: liver cells

text: Shuangyang sika deer liver lambda gt10 cDNA library

text: lambda gt10


text: live

text: Generic

characteristic: NCBI submission package

text: adult

text: Cervus nippon

text: male

text: Shuangyang sika deer

text: bone marrow

text: Shuangyang sika deer bone marrow PCR cDNA library

text: TA vector


text: University Cl. Bernard,

text: live

text: Generic

text: cultured ciliated epithelial cells after in vitro ciliogenesis

characteristic: cell type

text: cloned RT-PCR fragment from isolated total RNA

text: Adult

text: Homo sapiens

text: Nasal biopsy

text: ciliated epithelial cDNA cell library


text: Federal University of Minas Gerais

text: live

text: Generic

text: resting inflammatory macrophages

text: derived from DDRT-PCR

characteristic: description

text: Mus musculus

characteristic: strain

text: Mus musculus C3H/HeJ resting inflammatory macrophages


text: Federal University of Minas Gerais

text: live

text: Generic

text: IFN-gamma activated macrophages

text: derived from DDRT-PCR

text: Mus musculus

characteristic: organism

text: Mus musculus C3H/HeJ IFN-gamma activated macrophages


text: Federal University of Minas Gerais

characteristic: INSDC center name

text: live

text: Generic

text: resting Th2 cells

text: derived from DDRT-PCR

text: Mus musculus

text: Mus musculus C3H/HeJ resting Th2 cells


text: Federal University of Minas Gerais

text: live

text: Generic

text: Trypanosoma cruzi GPI-mucin activated macrophages

text: derived from DDRT-PCR

text: Mus musculus

text: Mus musculus C3H/HeJ GPI-mucin activated macrophages


text: University of California Irvine

text: live

text: Generic

text: Amblyomma americanum

text: midgut

text: Amblyomma americanum midgut


text: live

text: Generic

text: 1st strand cDNA was primed with a NotI - oligo(dT) primer 5'-AACTGGAAGAATTCGCGGCCGCGGCGCTTTTTTTTTTTTTTTTTTT-3'; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' AND 5'-CCTCGTGCCG-3'(Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library went through one round of normalization, and was constructed in the laboratory of M. Bento Soares (University of Iowa).

text: embryo, 14 dpc

text: DH10B (phage-resistant)

characteristic: lab-host

text: brain

text: Mus musculus

text: NotI

text: EcoRI

text: pituitary gland

text: Soares NMBP2 pituitary

text: phagemid (ampicillin resistant)

characteristic: vector


text: Dept. of Biochemistry, Medical College, Tongji University

characteristic: INSDC first public

text: live

text: Generic

text: 1 day postnatal

text: Rattus norvegicus

text: female and male

text: cerebellum

text: Differentially expressed cDNA library of cerebellum of rat


text: FBN Dummerstorf

text: live

text: Generic

text: juvenile

text: Sus scrofa

text: male

text: Liver

text: pig pUC18 Library

characteristic: title

text: Plasmid

text: pUC18 Sma I/BAP


text: Christchurch School of Medicine, University of Otago

characteristic: INSDC last update

text: live

text: Generic

text: Liver cell

text: Library prepared using Stratagene ZAP cDNA synthesis kit, catalogue #200450.

text: Adult

text: E.coli

text: Liver

characteristic: organ

text: Eptatretus cirrhatus

text: Eco RI

text: Xho I

text: Female

text: Liver

text: Hagfish liver Lambda ZAP cDNA library

text: Phagemid

text: pBluescript phagemid


text: live

characteristic: INSDC status

text: Generic

text: Liver cell

text: Library prepared using Stratagene ZAP-cDNA synthesis kit, catalogue #200450.

text: Adult

text: E.coli

text: Liver

text: Geotria australis

text: Eco RI

text: Xho I

text: Liver

text: Lamprey liver Lambda ZAP cDNA library

text: Phagemid

text: pBluescript phagemid


text: live

text: Generic

text: Liver cell

text: Adult

characteristic: dev-stage

text: E.coli

text: Liver

text: Sphenodon punctatus

text: Eco RI

text: Xho I

text: Male

characteristic: sex

text: Liver

text: Tuatara liver Lambda ZAP cDNA library

text: Phagemid

text: pBluescript phagemid


characteristic: External Id

text: Stockholm University

text: live

text: Generic

text: random primed cDNA.

text: last larval instar

text: Ephestia kuehniella

text: Ephestia kuehniella larval cDNA library

text: Phage

text: lambda gt11


text: Shanghai Institute of Planned Parenthood Research

text: live

text: Generic

characteristic: NCBI submission model

text: Total RNA was isolated from day 4 and day 5 embryos using Trizol (Gibco/BRL) procedure. cDNA was synthesized from total RNA primed by oligo(dT)15+18 and MMLV Reverse Transcriptase (Gibco/BRL). Differential display Experiments were preformed. Interesting amplified products were excised from PAGE, re-amplified, subcloned into pUCmT Vector (BBST, China) and sequenced.

text: day 4 or day 5 embryos

text: Mus musculus

text: ICR

text: Mouse day 4 or day 5 embryos


text: University of Massachusetts

text: live

text: Generic

characteristic: NCBI submission package

text: cDNA fragments isolated by differential display RT-PCR

text: Rattus norvegicus

text: Long Evans

text: cerebral cortex

characteristic: tissue-type

text: Rattus norvegicus cerebral cortex Long Evans G13


text: University of Massachusetts

text: live

text: Generic

characteristic: description

text: Rattus norvegicus

text: Long Evans

text: cerebral cortex

text: Rattus norvegicus cerebral cortex Long Evans G16


text: University of Delaware

text: live

text: Generic

text: E.coli EMDH10B

text: Gallus gallus

text: Male and Female

text: liver

text: Normalized Liver Library

text: pCMVSPORT 6


text: Cornell University

text: live

text: Generic

characteristic: cultivar

text: Directional

text: 5 weeks

text: Roots

text: Solanum lycopersicum

text: Roots

text: Tomato Root Subtraction cDNA Library

text: pBluescript KS


text: New Mexico State University

text: live

text: Generic

text: drought-stressed

text: leaf

text: Dactylis glomerata

characteristic: organism

text: Dactylis leaf DDRT-cDNA

text: plasmid

text: pGEM-T


text: New Mexico State University

characteristic: INSDC center name

text: live

text: Generic

text: drought-stressed

text: leaf

text: Dactylis glomerata

text: Xho 1

text: Dactylis leaf cDNA

text: Phage

text: Uni-ZAP XR


text: New Mexico State University

text: live

text: Generic

text: drought-stressed

text: leaf

text: Trifolium purpureum

text: Xho 1

text: Trifolium purpureum cDNA

text: Phage

text: Uni-ZAP XR


text: Aristotle University of Thessaloniki

text: live

text: Generic

text: Detection of low oxygen-responsive messengers expressed in Citrus flavedo cells using differential display RT-PCR

text: Citrus sinensis

text: Flavedo

text: Citrus sinensis flavedo cells

text: plasmid

text: pGEM T easy


text: University Paris12-Val de Marne

text: live

text: Generic

text: Darmor

text: In Brassica napus and related species, lateral roots emerging under drought conditions remain short (1-2 mm) and hairless (Vartanian et al., 1994). A substractive cDNA library was constructed (CLONTECH PCR-Select cDNA Subtraction Kit), using mRNA of short roots to synthetize the tester cDNA, and cDNA obtained from fully developped droughted lateral roots as driver. Differential expression was confirmed by hybridization with cDNA probes. The method used yielded RsaI digested cDNA fragments of sizes ranging from 150 to 800 bp, which were fully sequenced. Each sequence has significant similarity matches within the Arabidopsis genome.

text: Third leaf stage

characteristic: lab-host

text: lateral roots

text: Brassica napus

text: T/A cloning

text: Oleifera

characteristic: strain

text: Short roots substractive library

text: Plasmid

text: pT-Adv (Clontech)


text: Institute of Geriatric Cardiology, The General Hospital of PLA

characteristic: INSDC first public

text: live

text: Generic

text: smooth muscle cell

characteristic: cell type

text: Bos taurus

text: aorta

text: bovine aorta express library

text: Cosmid

characteristic: vector


text: CNRS, Institut Fessard

characteristic: INSDC last update

text: live

text: Generic

text: Oryzias latipes

text: anterior head

text: Oryzias latipes anterior head 30-31

characteristic: title


text: University of Edinburgh

text: live

characteristic: INSDC status

text: Generic

text: Ascaris suum is a parasite of pigs. The library was constructed from mRNA from Ascaris suum L3 stage.

characteristic: dev-stage

text: Ascaris suum

text: EcoRI (5'end)

text: mixed

text: Ascaris suum (parasitic nematode) L3 stage

text: phage

text: Lambda ZAP II


characteristic: External Id

text: University of Edinburgh

text: live

text: Generic

text: Ostertagia ostertagi is a parasite of cattle. The library was constructed from mRNA from Ostertagia ostertagi adults and produced with random hexamer primers.

text: adult

text: Ostertagia ostertagi

text: EcoRI (5'end)

text: NotI (3'end)

text: mixed

characteristic: sex

text: Ostertagia ostertagi adults

text: phage

text: Lambda gt11


text: Faculty of Veterinary Medicine

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Ostertagia ostertagi is a parasite of cattle. The library was constructed from mRNA from Ostertagia ostertagi L3 stage and produced with a poly-dT primer.

text: Ostertagia ostertagi

text: EcoRI (5'end)

text: NotI (3'end)

text: mixed

text: Ostertagia ostertagi L3

text: phage

text: Lambda gt11


text: University of Edinburgh

text: live

text: Generic

text: Ostertagia ostertagi is a parasite of cattle. The library was constructed from mRNA from Ostertagia ostertagi L4 stage and produced with a poly-dT primer.

characteristic: description

text: Ostertagia ostertagi

text: EcoRI

text: EcoRI

text: mixed

text: Ostertagia ostertagi L4

text: phage

text: Lambda ZAP


text: University of Edinburgh

text: live

text: Generic

text: Teladorsagia circumcincta is a parasitic nematode. The library was constructed from mRNA from Teladorsagia circumcincta mixed adults.

text: adult

text: Teladorsagia circumcincta

text: mixed

text: Teladorsagia circumcincta adults

text: phage

text: Lambda ZAP Express


text: Clemson University

text: live

text: Generic

text: Trump 545

characteristic: cultivar

text: Plants, grown in greenhouse for one month, were subjected to drought withholding watering. RNA was extracted from the leaves and differential display-PCR was used to identify cDNA up-regulated or down-regulated by drought-stress.

text: One-month-old

text: Leave

characteristic: organ

text: Helianthus annuus

text: Sunflower drought-stressed leaves


text: Clemson University

characteristic: INSDC center name

text: live

text: Generic

text: Trump 545

text: One-week-old seedlings were grown in 250mM NaCl for up to 9 hours. RNA was extracted separately from the roots and shoots, and differential display-PCR was used to identify cDNA up-regulated or down-regulated by salinity-stress.

text: One-week-old seedlings

text: Seedlings

text: Helianthus annuus

characteristic: organism

text: Sunflower seedlings


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: cDNA made by oligo-dT priming. Directionally cloned into EcoRI/XhoI sites using the following 5' adaptor: GGCACGAG(G). Library constructed by Ling Hong in the laboratory of Gerald M. Rubin (University of California, Berkeley) using ZAP-cDNA synthesis kit (Stratagene) and Superscript II RT (Life Technologies). Note: this is a NIH_MGC Library.

text: DH10B (phage-resistant)

characteristic: lab-host

text: blood

text: Homo sapiens

text: XhoI

text: EcoRI

text: natural killer cells, cell line

characteristic: tissue-type

text: plasmid (chloramphenicol resistant, grown at 30C)


characteristic: INSDC first public

text: live

text: Generic

text: DH10B (phage-resistant)

text: spleen

text: Homo sapiens

text: XhoI

text: EcoRI


text: Washington University School of Medicine

text: live

text: Generic

text: The library was constructed by Brandi Chiapelli and Dr. James McCarter at Washington University, St. Louis. The cDNA was made by using Dynabead oligo-dT priming (Dynal). PCR based library using a modified protocol from the SMART PCR cDNA Synthesis Kit from Clontech. Directionally cloned into the UDG sites of pAMP1. Nematodes were provided by Dr. David Bird of North Carolina State University.

text: enriched for eggs

text: Meloidogyne javanica

text: Meloidogyne javanica egg pAMP1 v6 Chiapelli McCarter

text: plasmid (ampicillin resistant)

text: pAMP1 (Gibco)

characteristic: vector


text: Shiyi Clinic Medicine School, Fudan University

characteristic: INSDC last update

text: live

text: Generic

text: isolated by differential display

text: Homo sapiens

text: Homo sapiens differential display (Yong LL)


text: Carnegie Mellon University

text: live

text: Generic

text: primary mesenchyme cells

characteristic: cell type

text: oligo dt priming from poly A+ RNA, directionally cloned

text: E.coli

text: Strongylocentrotus purpuratus

text: NotI

text: SalI

text: embryo

text: Sea urchin primary mesenchyme cell cDNA library

characteristic: title

text: Plasmid


text: RIKEN Institute

text: live

characteristic: INSDC status

text: Generic

text: country=Japan

text: Pyricularia grisea

characteristic: strain

text: Appressorium differentiation-stage specific differential cDNA library


characteristic: External Id

text: University of Texas Southwestern Medical Center

text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. RNA Isolation: cytoplasmic RNA preps (Manniatis); Cloning Technique: CUA Cloning (CloneAmp, Life Technologies); Average insert size: 1.8 Kb; Insertion site: TACGTCCACTGAATTCTGAGTG--->. Other information regarding entire library may be found at http://pga.swmed.edu/Data/Libraries/microarray_cdna_libraries.htm.

text: 2 months

characteristic: dev-stage

text: Mus musculus

text: Pooled

characteristic: sex

text: Cardiac muscle

text: UTSW Adult Mouse Cardiac Muscle Library

text: Plasmid

text: pAMP10 (Gibco)


text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Skeletal muscle

characteristic: description

text: 2 months

text: Mus musculus

text: Pooled

text: Diaphragm/Hind limb muscles

text: UTSW Adult Mouse Skeletal Muscle Library

text: Plasmid

text: pAMP10 (Gibco)


text: Shanghai Institute of Planned Parenthood Research

characteristic: INSDC center name

text: live

text: Generic

text: The Clontech PCR-Select cDNA subtraction kit was used to construct the subtracted cDNA library according to its user manual. Tester ds cDNA were prepared from the mRNA sample of mouse implantation-site uterus plus embryo at embryonic day 4.5 post-fertilization. Driver ds cDNA were prepared from the mRNA samples of mouse D4.5 inter-implantation-site uterus and D5.5 embryo.

text: embryonic day 4.5 post-fertilization

text: TOP10F E. coli

text: Mus musculus

text: female

text: BALB/c

text: Implantation-site uterus and embryo

text: Mouse implantation-site uterus plus embryo library


text: Harvard University, Howard Hughes Medical Institute

text: live

text: Generic

text: The library was prepared by Catherine S. Lee and has not been published. The pancreas was obtained from Gerard Gradwohl (PNAS 97 P1607-1611, 2000). The cDNA's were prepared with an oligo containing a NotI site, and SalI linkers were added to the ends. The inserts were cut with NotI before being cloned into the NotI-SalI sites in the vectors. This is one of two libraries, ngn3 wt and ngn3 -/-. The ngn3 -/- library is in pSPORT2, T7 promoter is 3'.

text: p.c. 14.5

text: E. coli-DH12S (GIBCO)

text: pancreas

characteristic: organ

text: Mus musculus

characteristic: organism

text: Not I

text: Sal I

text: 129/Sv x CD1

text: Kaestner ngn3 - -

text: plasmid

text: pSPORT2 (GIBCO)


text: live

text: Generic

text: The library was prepared by Catherine S. Lee and has not been published. The pancreas was obtained from Gerard Gradwohl (PNAS 97 P1607-1611, 2000). The cDNA's were prepared with an oligo containing a NotI site, and SalI linkers were added to the ends. The inserts were cut with NotI before being cloned into the NotI-SalI sites in the vectors. This is one of two libraries, ngn3 wt and ngn3 -/-. The wt library is in pSPORT1, T7 promoter is 5'.

text: p.c. 14.5

text: E. coli-DH12S (GIBCO)

characteristic: lab-host

text: pancreas

text: Mus musculus

text: Not I

text: Sal I

text: 129/Sv x CD1

text: Kaestner ngn3 wt

text: plasmid

text: pSPORT1 (GIBCO)


characteristic: INSDC first public

text: live

text: Generic

text: Library constructed using SuperScript Plasmid Library kit (Life Technologies). cDNA made by oligo-dT priming. Size-selected by column fractionation; average insert size 1.47 kb. Amplified once on solid support. cDNA Library Preperation: Guolin Chen.

text: Embryonic day 10.5 and 12.5, mixed

text: Pancreas

text: Mus musculus

text: Not 1

text: Sal 1

text: Both

text: ICR

text: Pancreatic Bud

characteristic: tissue-type

text: Melton Amplified Mouse E10 5 12 5 Pancreas 1 M10S1-A

text: plasmid (ampicillin resistant)


text: University of Texas M.D. Anderson Cancer Center

characteristic: INSDC last update

text: live

text: Generic

text: embryonic day 14.5 post-fertilization

text: Mus musculus

text: neural retina

text: Mouse E14.5 retina lambda ZAP II Library


text: North Carolina State University

text: live

text: Generic

text: The appressorium formation-specific cDNA library was constructed from conidia germinated for 5-8 hr on an inductive surface. The library contains over 55,000 clones with average insert size of 1.5 kbp.

text: Germinated conidia on apprressorium-inductive surface

text: Pyricularia grisea

text: EcoRI

text: XhoI

text: Magnaporthe grisea Appressorium Stage cDNAs localized on chromosome 7

text: Plasmid

text: pBlueScript SK(+) Vector

characteristic: vector


text: North Carolina State University

text: live

text: Generic

text: Pyricularia grisea

text: EcoRI

text: XhoI

text: Magnaporthe grisea Appressorium Stage cDNA

characteristic: title

text: Plasmid

text: pBlueScript SK(+) Vector


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

characteristic: INSDC status

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.5 kb. Constructed by Life Technologies. Note: This is a Xenopus Gene Collection (XGC) library.

text: adult

text: DH10B (phage-resistant)

text: brain

text: Xenopus laevis

text: NotI

text: SalI

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 2.3 kb. Constructed by Life Technologies. Note: This is a Xenopus Gene Collection (XGC) library.

text: adult

text: DH10B (phage-resistant)

text: eye

text: Xenopus laevis

text: NotI

text: SalI

text: phagemid (ampicillin resistant)


text: National Veterinary Institute

text: live

text: Generic

text: The Sarcoptes scabiei mixed lifestage library was constructed by Jens G Mattsson. cDNAs were synthesized from poly(A)+ RNA by oligo d(T) priming, size-selected and directionally cloned into the Uni-ZAP lambda vector (Stratagene). The primary library was amplified on XL1-Blue MRF' cells.

text: Sarcoptes scabiei

text: S.scabiei cDNA library


text: Yonsei Univ. College of Medicine

text: live

text: Generic

text: embryonic day 12-18

text: Rattus norvegicus

text: Sprague-Dawley

characteristic: strain

text: Rattus norvegicus Sprague-Dawley embryonic day 12-18


characteristic: External Id

text: Weill Medical College of Cornell University

text: live

text: Generic

text: thymocyte

characteristic: cell type

text: Random primed cDNA prepared by James Allen in 1992

text: SOLR

text: thymus

text: Mus musculus

text: thymus

text: Thymus Custom-made Lamda ZAP

text: Phagemid

text: pBluescript


text: Stanford University

text: live

text: Generic

text: BMS (Black Mexican Sweet)

characteristic: cultivar

text: The library was prepared by George Rudenko using poly (A) selected RNA and Universal Riboclone cDNA Synthesis System (Promega). cDNA was synthesized using both random and oligo(dT) primers in separate reactions and equipped with EcoRI adaptors. Library was size-fractionated on agarose gels (for insert size >400bp) and non-directionally cloned into EcoRI-digested pUC19 vector. Blue/white selection on carbenicillin-containing plates was used to recover positive clones.

text: mixed logarithmic and stationary growth phases

characteristic: dev-stage

text: Zea mays

text: EcoRI

text: EcoRI

text: suspension culture

text: 951 - BMS tissue from Walbot Lab (GR)

text: plasmid


text: Stanford University School of Medicine

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: The Clontech PCR-Select cDNA subtraction kit was used to construct the subtracted cDNA library. Tester DNA was prepared from the double sorted hematopoietic stem cells (c-kit+;Sca1+;Thy1.1low;Lin-). Driver DNA was prepared from the bone marrow.

text: adult 6-8 weeks

text: TOP10 E.coli

text: Mus musculus

text: TA cloning

text: AKR/J

text: Bone marrow

text: HB subtracted library

text: pTAdv


text: Agriculture and Agri-food Canada

characteristic: INSDC center name

text: live

text: Generic

text: Field-grown unpollinated maize ears were silk channel-inoculated in the morning (~10 am) with 1 ml of a Fusarium graminearum macroconidial suspension (500,000 spores/ml) and whole ears were collected and immediately frozen in liquid nitrogen 48 hours later.

characteristic: description

text: 6-7 days post-silk emergence

text: Zea mays

text: EcoRI

text: XhoI

text: 2.5 cm of top of unfertilized ear

text: Zm06_AAFC_ECORC_Fusarium_graminearum_inoculated_corn_eartip

text: Plasmid

text: Bluescript SK+/XhoI-EcoRI


text: Agriculture and Agri-food Canada

text: live

text: Generic

text: Field-grown maize ears were silk channel-inoculated in the morning (~10 am) with 1 ml of a Fusarium graminearum macroconidial suspension (500,000 spores/ml) and whole ears were collected and immediately frozen in liquid nitrogen 6 days later.

text: 10-11 days post-silk emergence

text: Zea mays

characteristic: organism

text: EcoRI

text: XhoI

text: Developing kernels (sibcrossed)

text: Zm08_AAFC_ECORC_Fusarium_graminearum_inoculated_corn_ear

text: Plasmid

text: Bluescript SK+/XhoI-EcoRI


text: Agriculture and Agri-food Canada

text: live

text: Generic

text: Field-grown corn was silk channel-inoculated in the morning (~10 am) with 1 ml of a macroconidial suspension (500,000 spores/ml) of Fusarium graminearum and silk channels were collected and immediately frozen in liquid nitrogen 6 hours later. RNA was extracted from silk tissue between 1 cm below and above the inoculation point in the silk channel, RNA from five silk channels was pooled.

text: 4-5 days post-silk emergence

text: Zea mays

text: EcoRI

text: XhoI

text: Silk

text: Zm10_AAFC_ECORC_Fusarium_graminearum_corn_silk

text: Plasmid

text: Bluescript SK+/XhoI-EcoRI


text: Agriculture and Agri-food Canada

text: live

text: Generic

text: Maple Arrow

text: Plants were grown 12 days from seeds, treated at 2oC for 3 days, then leaves were collected. Library was prepared using Unizap ZAP-cDNA synthesis kit / Packaged Gigapack III Gold.

text: 15-day seedlings

text: Glycine max

text: EcoRI

text: XhoI

text: Leaves

text: Gm01_AAFC_ECORC_Glycine_max_cold_stressed_leaves

text: Plasmid

text: Bluescript SK-/XhoI-EcoRI


text: Agriculture and Agri-food Canada

text: live

text: Generic

text: Plants incubated at 2 degrees under 12 hours of light/day. Harvested after only 2-3 days of cold treatment. cDNA was prepared with the Uni-Zap cDNA kit from Stratagene. Eco RI adapters were linked followed by digest with Xho I/Eco RI and ligated to pBluescript.

text: Glycine clandestina

text: EcoRI

text: XhoI

text: Leaves, stem

text: Gc01_AAFC_ECORC_cold_stressed_Glycine_clandestina

text: Plasmid

text: Bluescript SK+/XhoI-EcoRI


text: Agriculture and Agri-food Canada

text: live

text: Generic

text: Plants incubated at 2 C under 12 hours light/day and harvested after 3 days of cold treatment. SSH (Subtraction Suppressive Hybridization) library made using Clontech PCR-Select cDNA subtraction kit. PCR amplicons were ligated into the pGEM-Teasy vector (Promega).

text: Glycine clandestina

text: EcoRI

text: Leaves, stem

text: Gc02_AAFC_ECORC_cold_stressed_Glycine_clandestina_SSH

text: Plasmid

text: pGEM-T easy


text: Iowa State University

text: live

text: Generic

text: ds-cDNA molecules were generated as follows. First-strand cDNA was prepared from oligo-dT selected mRNA by priming with a NotI oligo-dT primer (5' AACTGGAAGAATTCGCGGCCGCAGGAATTTTTTTTTTTTTTTTTT). The resulting DNA:RNA hybrid was treated with RNase H and used as a template for DNA PolI-catalyzed second strand synthesis. After the addition of EcoRI adaptors, the ds-cDNAs were digested with NotI and size-selected. The resulting molecules were directionally cloned into the EcoRI and NotI sites of the pT7T3PAC vector.

characteristic: lab-host

text: Zea mays

text: EcoRI

text: NotI

text: Seedling and silk

text: Phagemid


text: Iowa State University

characteristic: INSDC first public

text: live

text: Generic

text: ds-cDNA molecules were generated as follows. First-strand cDNA was prepared from oligo-dT selected mRNA by priming with a NotI oligo-dT primer (5' AACTGGAAGAATTCGCGGCCGCAGGAATTTTTTTTTTTTTTTTTT). The resulting DNA:RNA hybrid was treated with RNase H and used as a template for DNA PolI-catalyzed second strand synthesis. After the addition of EcoRI adaptors, the ds-cDNAs were digested with NotI and size-selected. The resulting molecules were directionally cloned into the EcoRI and NotI sites of the pT7T3PAC vector. The library then went through one round of normalization to CoT value of 5 based on the methods of Marcelo Bento Soares (Genome Research 6: 791-806, 1996).

text: Zea mays

text: EcoRI

text: NotI

text: Seedling and silk

text: Phagemid


text: AgResearch

text: live

text: Generic

text: E. coli XLOLR

text: Brain

characteristic: organ

text: Ovis aries

text: EcoRI

text: XhoI

text: JB Sheep

text: Phagemid

text: pBK-CMV


text: Max-Planck-Institute for Cell Biology

characteristic: INSDC last update

text: live

text: Generic

text: 2d old roots

text: Helianthus annuus

text: sunflower Lambda UniZAP XR Express Library

text: UniZAP XR


text: Federal University of Minas Gerais

text: live

text: Generic

text: T cells

text: unstimulated Th2

characteristic: cell-line

text: Mus musculus

text: Mus musculus unstimulated Th2


text: People's Hospital, Peking University

text: live

text: Generic

text: squamous cell carcinoma

text: Homo sapiens

text: lung

characteristic: tissue-type

text: Homo sapiens lung squamous cell carcinoma

characteristic: title


text: Stanford University

text: live

characteristic: INSDC status

text: Generic

text: Unamplified cDNA library directionally cloned by Rima Kulikauskas using Stratagene's Uni-Zap system. Insert sizes ranged from 0.5Kb to 2Kb. 50 microliter aliquot had 338,000 pfu when it was made in Sept, 1995, from oligo dT-primed poly A+ RNA.

text: mature

text: SOLR

text: Zea mays

text: EcoRI (5-prime)

text: XhoI (3-prime)

text: pollen

text: 950 - Mature pollen from Sheila McCormick's lab

text: phagemid

text: Stratagene's Uni-Zap XR (pBluescript SK-)

characteristic: vector


characteristic: External Id

text: The Institute for Genomic Research (TIGR; www.tigr.org)

text: live

text: Generic

text: Kennebec

text: This library targets genes expressed in dormant tubers. This library was made from sections of dormant tuber, avoiding the buds and epidermis. Tubers were stored for one month post-harvest at 4oC. The tuber was peeled, well away from the surface. Then it was chopped into 1-2 mm cubes and immediately frozen in liquid nitrogen. This library is noted as P4 in Tanksley lab notebooks.

text: one month post-harvest

text: SOLR

text: Solanum tuberosum

text: EcoRI

text: XhoI

text: dormant tuber

text: cSTD

text: pBluescript SK(-)


text: Institute of Oceanology, Chinese Academy of Sciences

text: live

text: Generic

text: cDNA was prepared from polyA+ enriched RNA from the whole cephalothorax of a adult female shrimp Penaeus chinensis. The cDNA was directionally ligated into the pUC18 RS (SmaI/EcoRI) vector and transformed electrically into E. coli DH5a. Plasmids containing cDNA inserts were extracted from the recombinant pUC18 and propagated in DH5a cells.

text: E. coli strain DH5a

text: Penaeus chinensis

text: SmaI

text: EcoRI

text: Penaeus chinensis' cephalothorax cDNA library

text: plasmid


text: University of Florida College of Veterinary Medicine

text: live

text: Generic

text: Rattus norvegicus

text: Male

characteristic: sex

text: Brown Norway

characteristic: strain

text: Lung

text: subtracted cDNA library of BN rats following parainfluenza type 1 (Sendai) virus infection

text: Plasmid

text: pT-Adv


text: Academia Sinica

text: live

text: Generic

characteristic: NCBI submission model

text: sporophyte

characteristic: dev-stage

text: Marsilea quadrifolia

text: vegetative shoot apex

text: Marsilea quadrifolia vegetative shoot apex


text: Washington University School of Medicine

characteristic: INSDC center name

text: live

text: Generic

characteristic: NCBI submission package

text: The library was constructed by Brandi Chiapelli and Dr. James McCarter at Washington University, St. Louis. The cDNA was made by using Dynabead oligo-dT priming (Dynal). PCR based library using a modified protocol from the SMART PCR cDNA Synthesis Kit from Clontech. Directionally cloned into the UDG sites of pAMP1. Nematodes were provided by Dr. Mark Viney of Bristol, UK.

text: Strongyloides ratti

text: Strongyloides ratti L1 pAMP1 v3 Chiapelli McCarter

text: plasmid

text: pAMP1 (Gibco)


text: GlaxoSmithKline

text: live

text: Generic

text: Directional

characteristic: description

text: E.coli DH10 B

text: Homo sapiens

characteristic: organism

text: SalI

text: NotI

text: cartilage

text: HOA (Human Osteoarthritic Cartilage)

text: Plasmid

text: pSPORT I


text: University of Durham

characteristic: INSDC first public

text: live

text: Generic

text: Adult

text: Vibrissa Follicle

text: Rattus norvegicus

text: EcoRI

text: XhoI

text: PVG/c

text: Germinative epidermis

text: Rat GE subtracted cDNA library

text: Plasmid


text: live

text: Generic

text: Epithelium

characteristic: cell type

text: Anchor enzyme: NlaIII~ Tissue supplier: Jinsong Liu, M.D.~Tissue description: Ovarian cancer epithelium~Library treatment: non-normalized~Ovarian cancer library constructed in the Cancer Genomics Core Lab (M.D.Anderson Cancer Center)

text: ToP10 cell

characteristic: lab-host

text: Ovarian

characteristic: organ

text: Homo sapiens

text: NlaIII

text: Female

text: Ovarian Carcinoma

text: PZERO


text: Cereal Research Centre, Agriculture and Agri-food Canada

characteristic: INSDC last update

text: live

text: Generic

text: Thatcher Lr1

characteristic: cultivar

text: mRNA obtained from wheat NIL Thatcher Lr1 24 hours after inoculation with leaf rust pathogen Puccinia triticina race BBB carrying the avirulence gene Avr1.

text: 14 Days old

text: E. coli XLOLR

text: Triticum aestivum

text: EcoRI

text: XhoI

text: Leaf tissue

characteristic: tissue-type

text: Lambda ZapII; mass excised in plasmid vector pBK-CMV (Stratagene).


text: GlaxoSmithKline

text: live

characteristic: INSDC status

text: Generic

text: Directional

text: E.coli DH10 B

text: Homo sapiens

text: SalI

text: NotI

text: cartilage

text: HNC (Human Normal Cartilage)

characteristic: title

text: Plasmid

text: pSPORT I

characteristic: vector


characteristic: External Id

text: Johns Hopkins School of Medicine

text: live

text: Generic

text: Fetal

text: Brain

text: Homo sapiens

text: Human Fetal cDNA Library

text: Plasmid


text: Universiti Kebangsaan Malaysia

text: live

text: Generic

characteristic: NCBI submission model

text: Sporozoites were excysted in vitro from E. tenella H oocysts, purified by column chromatography and mRNA extracted using a FASTTRACK kit (Invitrogen). cDNA was synthesised and a Uni-ZAP XR library was constructed using cDNA synthesis kit, ZAP-cDNA synthesis kit and ZAP-cDNA Gigapack III Gold cloning kit (Stratagene). The library was amplified once through E. coli XL1-Blue MRF'.

text: Sporozoite

text: XL1-Blue MRF'

text: Eimeria tenella

text: EcoRI

text: XhoI

text: Houghton

characteristic: strain

text: Lambda ZAPII