text: University of Naples Federico II

text: live

text: Generic

characteristic: NCBI submission package

text: Total RNA was isolated and subsequently pooled from ovaries (17 days after pollination, 0.5x), flowerbuds (1x), calli (1x), inflorescences (1x) and seedlings (0.5x) of Petunia axillaris(the pooling ratios are given between brackets). Total RNA from the different plant tissues was isolated using Trizol reagent (Life Technologies, Cleveland, OH) according to the instructions of the manufacturer. An additional precipitation with Ethanol and sodium Acetate was performed to improve the 260/230 ratio. For the Petunia axillaris cDNA libraries preparation, we supplied Eurofins MWG Operon (Edersberg, Germany) with two high quality total RNA pool samples of 15 *g each, from which they prepared 3’-fragment cDNA libraries. These libraries consist of non restricted fragments from the 3’-end of each mRNA. The cDNA libraries preparation is in short described by Bellini et al., (2009). The resulting non-cloned libraries were normalised and subsequently sequenced on a half plate each, by GS FLX technology using Titanium series chemistry.

text: Petunia axillaris

text: RNA extracted from ovaries, flowerbuds, calli, inflorescences and seedlings of Petunia axillaris


status: PUBLIC

characteristic: External Id

text: Joint Genome Institute, U.S. Department of Energy

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

characteristic: INSDC secondary accession

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: SRA accession

text: Piloderma croceum F 1598

characteristic: description

text: Piloderma croceum F 1598

characteristic: organism

text: F 1598

tag: attribute

characteristic: strain

text: Generic sample from Piloderma croceum F 1598

characteristic: title


FGSC 8874

text: UC Berkeley

text: live

text: Generic

text: FGSC 8874/D114

text: Neurospora crassa

text: FGSC 8874


FGSC 8876

text: UC Berkeley

text: live

text: Generic

text: FGSC 8876/D116

text: Neurospora crassa

text: FGSC 8876


VAC V

text: NIAID

text: live

text: Generic

text: Vaccinia virus infection of Drosophila S9 cells at progessive times

text: Vaccinia virus

text: Vaccinia Virus


FGSC 8878

text: UC Berkeley

text: live

text: Generic

text: FGSC 8878/D118

text: Neurospora crassa

text: FGSC 8878


PRJNA167910.P.nyererei 1_Seehausen

text: Broad Institute

text: live

text: Generic

text: Male

characteristic: host sex

text: Pundamilia nyererei

text: P.nyererei 1_Seehausen

characteristic: sample name

text: Generic sample from Pundamilia nyererei


Mouse adult spleen

text: Institute of Molecular and Cell Biology (A*STAR)

text: live

text: Generic

text: EDGE libraries were generated from spleen RNA from five adult ( 8-week old ) C57BL/6J non-mutant and five C57BL/6J mutant (Mc1r e/e) animals. One library per animal.

text: Mus musculus

text: Mouse spleen EDGE


text: University of California - Davis, Drosophila Population Genomics Project

text: live

text: Generic

text: Drosophila melanogaster


text: University of Washington

text: live

text: Generic

text: Untreated sample. The 3134 cell line was derived by transformation of C127, originally isolated from a mammary adenocacinoma tumor of the RIII mouse. Cell lines were maintained in Dulbecco's Modified Eagle Medium (DMEM) (Invitrogen, Carlsbad, CA) supplemented with 10% fetal bovine serum (Gemini, Woodland, California), 2 mM L-glutamine, 1 mM sodium pyruvate, 0.1 mM non-essential amino acids, 5 mg/ml penicillin-streptomycin (Invitrogen, Carlsbad, CA) and kept at 37oC incubator with 5% CO2.

text: Mus musculus

text: 3134 cell line, -DEX replicate 2


HL1 cells

text: The Australian National University

text: live

text: Generic

text: Small RNA population derived from HL1 cells in culture at different time points after plating.

text: Mus musculus

text: miRNA profile of HL1 cardiomyocyte


text: University of Calgary

text: live

text: Generic

text: ESTs derived from stems of Chelidonium majus.

text: Chelidonium majus


text: University of Washington

text: live

text: Generic

text: Mus musculus

text: 3134 cell line, -DEX replicate 1


text: DOE JGI

text: live

text: Generic

text: High molecular weight DNA extracted from the post-incubated switchgrass adherent rumen microbiome was used as a template for three different sequencing libraries. Two whole genome shotgun sequencing libraries with insert size 200 bp and 300 bp were independently generated from DNA, followed by high-throughput sequencing from both ends, one by 2x125bp using Illumina GAIIx and one by 2x100bp using Illumina HiSeq2000 instruments. Two additional libraries were built with insert sizes of 3kb and 5kb respectively to facilitate genome assembly. Both of these two “jumping” libraries were sequenced using Illumina GAIIx by 2x75bp pair-end sequencing.

text: bovine gut metagenome

text: Metagenomic DNA from Switch grass fiber attached community in cow rumen, 72 hour post incubation


m3134-DS8500 DEX

text: University of Washington

text: live

text: Generic

text: Treated sample. The 3134 cell line was derived by transformation of C127, originally isolated from a mammary adenocacinoma tumor of the RIII mouse. Cell lines were maintained in Dulbecco's Modified Eagle Medium (DMEM) (Invitrogen, Carlsbad, CA) supplemented with 10% fetal bovine serum (Gemini, Woodland, California), 2 mM L-glutamine, 1 mM sodium pyruvate, 0.1 mM non-essential amino acids, 5 mg/ml penicillin-streptomycin (Invitrogen, Carlsbad, CA) and kept at 37oC incubator with 5% CO2. Cells were transferred to 10% charcoal-dextran-treated, heat-inactivated fetal bovine serum for 48 hrs prior to hormone treatment (1hr with 100 nM dexamethasone)

text: Mus musculus

text: 3134 cell line, plus DEX replicate 1


text: University of Washington

text: live

text: Generic

text: Mus musculus

text: 3134 cell line, plus DEX replicate 2


mouse neonate dermis RNA-Seq

text: live

text: Generic

text: 1 wild type and 1 mutant RNA-Seq library from mouse neonate dermis, each from 1 animal

text: Mus musculus

text: Mouse neonate dermis RNA-Seq


Cheetah skin EDGE

text: live

text: Generic

text: Two skin EDGE libraries from the same cheetah. One from black spot, one from yellow background.

text: Acinonyx jubatus

text: Cheetah skin EDGE


Mouse adult skin EDGE

text: live

text: Generic

text: Technical replicates generated from two pools of mouse adult skin RNA (3 animals per pool). One pool of RNA was from C57BL/6J non-mutant animals and one from C57BL/6J mutant (Mc1r e/e) animals. Two EDGE libraries were constructed from wild type and sequenced at two different sequencing centers (Stanford and HudsonAlpha). Two EDGE libraries were constructed from mutant and sequenced at two different sequencing centers (Stanford and HudsonAlpha).

text: Mus musculus

text: Mouse adult skin EDGE Technical Replicates


text: University of Washington

text: live

text: Generic

text: Untreated sample. The AtT-20 cell line is an anterior pituitary corticotroph of murine origin (ATCC). Cell lines were maintained in Dulbecco's Modified Eagle Medium (DMEM) (Invitrogen, Carlsbad, CA) supplemented with 10% fetal bovine serum (Gemini, Woodland, California), 2 mM L-glutamine, 1 mM sodium pyruvate, 0.1 mM non-essential amino acids, 5 mg/ml penicillin-streptomycin (Invitrogen, Carlsbad, CA) and kept at 37oC incubator with 5% CO2.

text: Mus musculus

text: AtT-20 cell line, -DEX replicate 1


text: University of Washington

text: live

text: Generic

text: Treated sample. The AtT-20 cell line is an anterior pituitary corticotroph of murine origin (ATCC). Cell lines were maintained in Dulbecco's Modified Eagle Medium (DMEM) (Invitrogen, Carlsbad, CA) supplemented with 10% fetal bovine serum (Gemini, Woodland, California), 2 mM L-glutamine, 1 mM sodium pyruvate, 0.1 mM non-essential amino acids, 5 mg/ml penicillin-streptomycin (Invitrogen, Carlsbad, CA) and kept at 37oC incubator with 5% CO2. Cells were transferred to 10% charcoal-dextran-treated, heat-inactivated fetal bovine serum for 48 hrs prior to hormone treatment (1hr with 100 nM dexamethasone)

text: Mus musculus

text: AtT-20 cell line, plus Dex replicate 1


text: University of Washington

text: live

text: Generic

text: Mus musculus

text: 3134 cell line, -DEX replicate 3


PRJNA59869.ID#1420 age 421 days

text: Broad Institute

text: live

text: Generic

text: Female

text: voucher

characteristic: isolation source

text: Mustela putorius furo

text: ID#1420 age 421 days

text: inbred

text: Generic sample from Mustela putorius furo


text: University of Washington

text: live

text: Generic

text: Mus musculus

text: 3134 cell line, -DEX replicate 4


text: University of Washington

text: live

text: Generic

text: Mus musculus

text: 3134 cell line, plus Dex replicate 3


text: University of Washington

text: live

text: Generic

text: Mus musculus

text: 3134 cell line, plus Dex replicate 4


text: University of Washington

text: live

text: Generic

text: Mus musculus

text: 3134 cell line, -DEX replicate 5


text: University of Washington

text: live

text: Generic

text: Mus musculus

text: 3134 cell line, plus DEX replicate 5


P. yessoensis transcriptome project

text: Ocean University of China

text: live

text: Generic

text: Mizuhopecten yessoensis

text: Submission Comments: Transcriptome sequencing and de novo analysis for Yesso scallop (Patinopecten yessoensis) using 454 GS FLX


Normal temporal lobe

text: UNSW

text: live

text: Generic

text: normal

text: Quantitative analysis polyA RNA from Normal human brain temporal lobe. The RNA was purchased from Capital Biosciences and is a pool of 5 samples originating from males aged 23 - 29yrs old.

text: Homo sapiens

text: brain temporal lobe

characteristic: tissue type

text: Human Brain Normal Temporal lobe


text: University of Washington

text: live

text: Generic

characteristic: NCBI submission package

text: Mus musculus

text: AtT-20 cell line, -DEX replicate 2


Normal frontal lobe

characteristic: External Id

text: UNSW

text: live

text: Generic

text: normal

text: Quantitative analysis of polyA selected RNA from Normal human brain frontal lobe. The sample was purchased from Capital Biosciences and is a pool of 5 samples belonging to males aged between 23 - 29 years old.

text: Homo sapiens

text: brain frontal lobe

text: Human Brain Normal Frontal lobe


text: University of Washington

characteristic: INSDC center name

text: live

text: Generic

text: Mus musculus

text: AtT-20 cell line, -DEX replicate 3


text: University of Washington

characteristic: INSDC first public

text: live

text: Generic

text: Mus musculus

text: AtT-20 cell line, plus Dex replicate 2


Normal total brain

text: UNSW

characteristic: INSDC last update

text: live

text: Generic

text: normal

text: Quantitative analysis polyA RNA from Normal human whole brain . The RNA was purchased from Ambion and is a pool of 13 male and 10 female samples. The ages of the males and females range from 23 to 86 years old.

text: Homo sapiens

text: brain, whole

characteristic: tissue

text: Normal Human whole brain


text: University of Washington

characteristic: INSDC secondary accession

text: live

characteristic: INSDC status

text: Generic

text: Mus musculus

text: AtT-20 cell line, plus Dex replicate 3


AD Temporal lobe

text: UNSW

text: live

text: Generic

characteristic: NCBI submission model

characteristic: SRA accession

text: affected

text: Quantitative analysis of polyA RNA from Alzheimer's disease human brain temporal lobe. The RNA was purchased from Capital Biosciences and belongs to a male aged 80 years old.

text: Alzheimer's disease

characteristic: disease type

text: Homo sapiens

text: brain temporal lobe

text: Human Alzheimer's disease brain, Temporal lobe


text: University of Washington

text: live

text: Generic

text: Untreated sample. The 3134 cell line was derived by transformation of C127, originally isolated from a mammary adenocacinoma tumor of the RIII mouse. Cell lines were maintained in Dulbecco's Modified Eagle Medium (DMEM) (Invitrogen, Carlsbad, CA) supplemented with 10% fetal bovine serum (Gemini, Woodland, California), 2 mM L-glutamine, 1 mM sodium pyruvate, 0.1 mM non-essential amino acids, 5 mg/ml penicillin-streptomycin (Invitrogen, Carlsbad, CA) and kept at 37oC incubator with 5% CO2.

characteristic: description

text: Mus musculus

characteristic: organism

text: AtT-20 cell line, -DEX replicate 4


text: University of Washington

text: live

text: Generic

text: Mus musculus

text: AtT-20 cell line, plus Dex replicate 4

characteristic: title


AD Frontal lobe

text: UNSW

text: live

text: Generic

text: affected

text: Quantitative analysis polyA RNA from Alzheimer's disease human frontal lobe. The RNA was purchased from Capital Biosciences and originates from a male aged 87 years old.

text: Alzheimer's

text: Homo sapiens

text: brain, frontal lobe

text: Human Alzheimer's disease brain, frontal lobe


AD Total brain

text: UNSW

text: live

text: Generic

text: affected

text: Quantitative analysis polyA RNA from Alzheimer's disease human total brain. The RNA was purchased from Capital Biosciences and originates from a male ages 87 years old.

text: Alzheimer's

text: Homo sapiens

text: brain, whole

text: Human Alzheimer's disease, whole brain


text: Broad Institute

text: live

text: Generic

text: human gut metagenome

characteristic: sample name

text: Generic sample from human gut metagenome


text: University of California, Santa Cruz

text: live

text: Generic

text: embryonic stem cells

characteristic: cell type

text: UNDIFF

characteristic: developmental stage

text: Mus musculus

text: stem cell culture

characteristic: strain

text: UNDIFF-PNK

text: PNK

characteristic: treatment group


characteristic: External Id

text: University of California, Santa Cruz

characteristic: INSDC center name

text: live

text: Generic

characteristic: NCBI submission package

text: embryonic stem cells

text: UNDIFF

text: Mus musculus

text: stem cell culture

text: UNDIFF-NUCLEASE

text: NUCLEASE


text: University of California, Santa Cruz

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: embryonic stem cells

text: UNDIFF

text: Mus musculus

text: stem cell culture

text: barcode03 (sample not used)


text: University of California, Santa Cruz

characteristic: INSDC secondary accession

text: live

characteristic: INSDC status

text: Generic

text: neural precursor cells

text: Mus musculus

text: stem cell culture

text: PNK


text: University of California, Santa Cruz

text: live

text: Generic

characteristic: NCBI submission model

characteristic: SRA accession

text: neural precursor cells

text: Mus musculus

text: stem cell culture

text: NUCLEASE


text: University of California, Santa Cruz

text: live

text: Generic

text: neural precursor cells

text: Mus musculus

text: stem cell culture

text: barcode06 (sample not used)


text: University of California, Santa Cruz

text: live

text: Generic

text: embryonic stem cells

text: UNDIFF

text: Mus musculus

text: stem cell culture

text: UNDIFF-CONTROL

text: CONTROL


text: University of California, Santa Cruz

text: live

text: Generic

text: neural precursor cells

text: Mus musculus

text: stem cell culture

text: CONTROL


text: University of California, Santa Cruz

text: live

text: Generic

text: embryonic stem cells

text: UNDIFF

text: Mus musculus

text: stem cell culture

text: barcode09 (sample not used)


text: University of California, Santa Cruz

text: live

text: Generic

text: embryonic stem cells

text: UNDIFF

text: Mus musculus

text: stem cell culture

text: barcode10 (sample not used)


text: Molecular Biology, Radboud University

text: live

text: Generic

text: : 10-12

text: Xenopus tropicalis

text: whole embryo


text: Molecular Biology, Radboud University

text: live

text: Generic

text: : oocyte

text: Xenopus tropicalis

text: whole embryo


text: Plant Sciences, UC Davis

text: live

text: Generic

text: Bobwhite

characteristic: cultivar

characteristic: genotype/variation

text: Triticum aestivum

text: flag_leaf, GPC_gene_silenced, cv_Bobwhite

text: 12 Days Post Anthesis

characteristic: stage

text: flag leaf

characteristic: tissue


text: Plant Sciences, UC Davis

text: live

text: Generic

text: Bobwhite

text: wild type

text: Triticum aestivum

characteristic: organism

text: 12 Days Post Anthesis

text: flag leaf

characteristic: title


text: Laboratory for Regenerative Medicine, Department of Surgery, University of Cambridge

text: live

text: Generic

text: H9 hESC

characteristic: cell line

text: embryonic stem cell

characteristic: developmental stage

text: Homo sapiens

text: Input DNA: H9 human embryonic stem cell (hESC)


characteristic: External Id

text: live

text: Generic

text: H9 hESC

text: EOMES

characteristic: chip antibody

text: Abcam (Cambridge, UK)

characteristic: chip antibody vendor

text: early endoderm 48h

text: Homo sapiens

text: EOMES ChIP fraction: early endoderm (hESC differentiated towards endodermal fate 48h)

text: hESC_48h-endodiff_EOMES_XL_ChIP-Seq_reps1and2


text: NIAAA/NIH

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

characteristic: NCBI submission package

text: Macaca mulatta

text: Mother-reared E20


text: NIAAA/NIH

characteristic: INSDC last update

characteristic: INSDC secondary accession

text: live

characteristic: INSDC status

text: Generic

text: Macaca mulatta

text: Mother-reared K09


text: NIAAA/NIH

text: live

text: Generic

characteristic: NCBI submission model

characteristic: SRA accession

text: Macaca mulatta

text: Mother-reared K20


text: NIAAA/NIH

text: live

text: Generic

text: Macaca mulatta

text: Mother-reared K29


text: NIAAA/NIH

text: live

text: Generic

text: Macaca mulatta

text: Mother-reared K44


text: NIAAA/NIH

text: live

text: Generic

text: Macaca mulatta

text: Mother-reared K46


text: NIAAA/NIH

text: live

text: Generic

text: Macaca mulatta

text: Peer-reared E13


text: NIAAA/NIH

text: live

text: Generic

text: Macaca mulatta

text: Peer-reared E78


text: NIAAA/NIH

text: live

text: Generic

text: Macaca mulatta

text: Peer-reared E85


text: NIAAA/NIH

text: live

text: Generic

text: Macaca mulatta

text: Peer-reared G36


text: NIAAA/NIH

text: live

text: Generic

text: Macaca mulatta

text: Peer-reared K41


text: NIAAA/NIH

text: live

text: Generic

text: Macaca mulatta

text: Surrogate-peer-reared G14


text: NIAAA/NIH

text: live

text: Generic

text: Macaca mulatta

text: Surrogate-peer-reared M18


text: Ari Melnick, Hematology/Oncology, WCMC

text: live

text: Generic

text: anti-BCL6 antibody (N3)

text: Santa Cruz

text: Homo sapiens

text: ChIPSeq, human CML cell line


text: Ari Melnick, Hematology/Oncology, WCMC

text: live

text: Generic

text: none (control)

text: Homo sapiens

text: Input, human CML cell line


text: NIAAA/NIH

text: live

text: Generic

text: Macaca mulatta

text: Surrogate-peer-reared M40


name: Caenorhabditis elegans N2 exposed to Drechmeria coniospora 12hrs RNA sample 2

text: University of Washington Genome Sciences

text: live

text: Generic

text: Adult C. elegans N2 fer-15 worms exposed to Drechmeria coniospora (DC) for 12 hours; 2nd assay of infected worms. Worms provided by Jonathan Ewbank. This sample is available through the modENCODE DCC (www.modencode.org) as: Adult_Drechmeria_coniospora_2_12_hrs Local library name: DC-2-12 The control worms for this experiment can be found in experiment OPDC-2-12, or through the DCC at Adult_Drechmeria_coniospora_control_EcoliOP50_exposed_2_12hrs. Quality values are ASCII encoded Phred + 33 through GAPipeline1.8.

characteristic: description

text: Caenorhabditis elegans


text: live

text: Generic

text: myoepithelial cell

characteristic: cell type

text: Tissue Description: normal mammary myoepithelial cells from breast reduction tissue of a 27 year old female. Library constructed in the laboratory of K. Polyak, M.D., Ph.D. (Dana-Farber Cancer Institute). Anchor enzyme: NlaIII

characteristic: lab-host

text: Homo sapiens

text: female

characteristic: sex

text: breast

characteristic: tissue-type

text: plasmid

characteristic: vector


characteristic: External Id

text: live

text: Generic

text: epithelium

text: Tissue Description: normal mammary epithelium (gestational hyperplasia) from a 31 year old breast cancer patient (20 weeks pregnant). Library constructed in the laboratory of K. Polyak, M.D., Ph.D. (Dana-Farber Cancer Institute). Anchor enzyme: NlaIII

text: Homo sapiens

characteristic: organism

text: female

text: breast

characteristic: title

text: plasmid


text: live

text: Generic

text: Tissue Description: ependymoblastoma primary tumor, WHO grade IV, from 9 month old female. Library constructed in the laboratory of G. Riggins, M.D., Ph.D. (Duke University). Anchor enzyme: NlaIII

text: brain

characteristic: organ

text: Homo sapiens

text: female

text: plasmid


characteristic: INSDC first public

text: live

text: Generic

text: epithelial cell

text: Tissue Description: normal gastric epithelial tissues (4 pooled) dissected from the body of the stomach, obtained by surgery. Library constructed using the standard SAGE protocol by S. Powell, M.D. and Wa'el El-Rifai, M.D., Ph.D. (Digestive Health Center, University of Virginia Health System). Anchor enzyme: NlaIII

text: stomach

text: Homo sapiens

text: plasmid


text: live

text: Generic

characteristic: NCBI submission package

text: Normal liver from 37 year old male. Library constructed in the laboratory of G. Riggins, M.D., Ph.D. (Duke University). Anchor enzyme: NlaIII

text: liver

text: Homo sapiens

text: male

text: plasmid


characteristic: INSDC last update

text: live

text: Generic

text: Ependymoma, recurrent brain tumor, from 6.3 year old female. Library constructed in the laboratory of G. Riggins, M.D., Ph.D. (Duke University). Anchor enzyme: NlaIII

text: brain

text: Homo sapiens

text: female

text: tumor

text: plasmid


text: live

text: Generic

text: granule cell precursor

text: Mouse primary granule cell precursors, isolated on postnatal day 8 and cultured for 18 hours in serum-free medium. Library constructed using the microSAGE protocol by Christopher Turner in the laboratory of G. Riggins, M.D., Ph.D. (Duke University). Anchor enzyme: NlaIII

text: Mus musculus

text: plasmid


text: live

text: Generic

text: Anchor enzyme: NlaIII~ Normal frontal cortex, bulk tissue, from 15 month male seizure patient (partial lobectomy). Library constructed by D. Olschner in the laboratory of G. Riggins, M.D., Ph.D. (Duke University).

text: brain

text: Homo sapiens

text: male

text: frontal cortex

text: plasmid


text: live

text: Generic

text: granule cell precursors

text: Anchor enzyme: NlaIII~ Granule cell precursor isolated on postnatal day 8 and cultured for 18 hr in serum-free medium containing 3 ug/ml sonic hedgehog. Library constructed by C. Turner in the laboratory of G. Riggins, M.D., Ph.D. (Duke University).

text: Mus musculus

text: plasmid


text: live

text: Generic

text: Anchor enzyme: NlaIII~ Glioma, primary tumor, from 4 year old female. Library constructed by B. Fee in the laboratory of G. Riggins, M.D., Ph.D. (Duke University).

text: brain

text: Homo sapiens

text: female

text: glioma

text: plasmid


text: live

text: Generic

text: Anchor enzyme: NlaIII~ Normal heart, from 56 year old male, cytomegalovirus positive. Total RNA purchased from Ambion, catalog #7966, lot #051P43C. Library constructed using the microSAGE technique by D. Olschner in the laboratory of G. Riggins, M.D., Ph.D. (Duke University).

text: heart

text: Homo sapiens

text: male

text: plasmid


text: live

text: Generic

text: Anchor enzyme: NlaIII~ Bulk ependymoma tissue from 34 year old female. Library constructed by B. Fee in the laboratory of G. Riggins, M.D., Ph.D. (Duke University).

text: brain

text: Homo sapiens

text: female

text: ependymoma

text: plasmid


text: live

text: Generic

text: Anchor enzyme: NlaIII~Human hemangioma tumor tissue from juvenile. Library constructed by Anita Lal in the laboratory of G. Riggins, M.D., Ph.D. (Duke University).

text: head/neck

text: Homo sapiens

text: hemangioma

text: plasmid


text: live

text: Generic

text: Anchor enzyme: NlaIII~ Bulk primary astrocytoma from 35 year old male. Library constructed using the microSAGE protocol by J. Edwards and K. Boon in the laboratory of G. Riggins, M.D., Ph.D. (Duke University).

text: brain

text: Homo sapiens

text: male

text: astrocytoma

text: plasmid


text: live

text: Generic

text: Anchor enzyme: NlaIII~ Pooled organs and tissues include: B-lymphocyte (plasmacytoma, mycloma), mammary gland (adenocarcinoma), liver (hepatoblastoma), cervix (adenocarcinoma), testis (embryonal carcinoma), brain (glioblastoma), melanoma, liposarcoma, macrophage (histiocytic lymphoma, histocyte), and T-lymphoblast (lymphoblastic leukemia). Universal reference RNA purchased from Stratagene, catalog #740000-41, lot #1000207. Library constructed using the microSAGE protocol by D. Olschner in the laboratory of G. Riggins, M.D., Ph.D. (Duke University).

text: pooled

text: Homo sapiens

text: pooled

text: plasmid


text: live

text: Generic

text: Invasive ductal carcinoma with some in situ component, low-grade, ER-positive, erbB2-negative, lymph node-negative, p53-negative. Library constructed in the laboratory of K. Polyak, M.D., Ph.D. (Dana-Farber Cancer Institute).

text: breast

text: Homo sapiens

text: carcinoma

text: plasmid


text: live

text: Generic

text: Ductal carcinoma in situ, high-grade, comedo from 32 yo woman. Library constructed in the laboratory of K. Polyak, M.D., Ph.D. (Dana-Farber Cancer Institute).

text: breast

text: Homo sapiens

text: carcinoma

text: plasmid


text: live

text: Generic

text: Human ependymoma bulk tumor tissue from 2.3 yo female. Library constructed by Brian Fee in the laboratory of G. Riggins, M.D., Ph.D. (Duke University).

text: brain

text: Homo sapiens

text: ependymoma

text: plasmid


text: live

text: Generic

text: Ductal carcinoma in situ, high-grade, comedo, from 41 yo female. Library constructed in the laboratory of K. Polyak, M.D., Ph.D. (Dana-Farber Cancer Institute).

text: breast

text: Homo sapiens

text: carcinoma

text: plasmid


text: live

text: Generic

text: Ductal carcinoma in situ, high-grade, comedo. Library constructed in the laboratory of K. Polyak, M.D., Ph.D. (Dana-Farber Cancer Institute).

text: breast

text: Homo sapiens

text: carcinoma

text: plasmid


text: live

text: Generic

text: Anchor enzyme: NlaIII~ Invasive ductal carcinoma, high-grade, ER-negative, erbB2-negative, lymph node-negative, p53-positive. Library constructed in the laboratory of K. Polyak, M.D., Ph.D. (Dana-Farber Cancer Institute).

text: breast

text: Homo sapiens

text: carcinoma

text: plasmid


text: live

text: Generic

text: Anchor enzyme: NlaIII~ Invasive ductal carcinoma, low-grade, ER-positive, erbB2-negative, lymph node-negative, p53-negative. Library constructed in the laboratory of K. Polyak, M.D., Ph.D. (Dana-Farber Cancer Institute).

text: breast

text: Homo sapiens

text: carcinoma

text: plasmid


text: live

text: Generic

text: Anchor enzyme: NlaIII~ Tissue Description: primary gastric cancer tissue, poorly differentiated, T4N0M0, obtained by surgery. Library constructed using the standard SAGE protocol by S. Powell, M.D. and Wa'el El-Rifai, M.D., Ph.D. (Digestive Health Center, University of Virginia Health System).

text: stomach

text: Homo sapiens

text: plasmid


text: live

text: Generic

text: Anchor enzyme: NlaIII~ Ependymoma, bulk tumor tissue, from 31 year old male. Library constructed by Brian Fee in the laboratory of G. Riggins, M.D., Ph.D. (Duke University).

text: brain

text: Homo sapiens

text: male

text: tumor

text: plasmid


text: live

text: Generic

text: Anchor enzyme: NlaIII~ Ependymoma, bulk tumor tissue, from 17 year old male. Library constructed by Brian Fee in the laboratory of G. Riggins, M.D., Ph.D. (Duke University).

text: brain

text: Homo sapiens

text: male

text: tumor

text: plasmid


text: live

text: Generic

text: Anchor enzyme: NlaIII; Human astrocytoma bulk primary tumor tissue from 5 yo female. Library constructed by Brian Fee in the laboratory of G. Riggins, M.D., Ph.D. (Duke University).

text: brain

text: Homo sapiens

text: astrocytoma

text: plasmid


text: live

text: Generic

text: Anchor enzyme: NlaIII; Astrocytoma, bulk primary tumor tissue, from 4 yo female. Library constructed using the Microsage technique by J. Edwards in the laboratory of G. Riggins, M.D., Ph.D. (Duke University).

text: brain

text: Homo sapiens

text: astrocytoma

text: plasmid


text: live

text: Generic

text: Anchor enzyme: NlaIII~ Astrocytoma, bulk primary tumor tissue, from 23 yo male. Library constructed using the Microsage technique by J. Edwards in the laboratory of G. Riggins, M.D., Ph.D. (Duke University).

text: brain

text: Homo sapiens

text: astrocytoma

text: plasmid


text: live

text: Generic

text: Spring wheat

characteristic: cultivar

text: Wheat (Triticum Aestivum l., Red Spring Wheat) kernel malted 55 hours at 22 C.

text: Triticum aestivum

text: EcoRI

text: XhoI

text: kernel

text: pBluescript SK+


text: live

characteristic: INSDC status

text: Generic

text: Wheat (Triticum aestivum)developing seedling full length library with out biotin

text: Triticum aestivum

text: EcoRI

text: XhoI

text: seedling

text: pBluescript SK+


text: live

text: Generic

characteristic: NCBI submission model

text: wheat kernel library (8-12DPA)

text: Triticum aestivum

text: EcoRI

text: XhoI

text: kernel

text: pBluescript SK+


text: live

text: Generic

text: 11 days pregnant adult

characteristic: dev-stage

text: Mus musculus

text: female

characteristic: strain

text: placenta

text: RIKEN full-length enriched, 11 days pregnant adult female placenta


text: live

text: Generic

text: 12 days pregnant adult

text: Mus musculus

text: female

text: placenta

text: RIKEN full-length enriched, 12 days pregnant adult female placenta


text: live

text: Generic

text: morula

text: Mus musculus

text: whole body

text: RIKEN full-length enriched, morula whole body


text: live

text: Generic

text: 10 days neonate

text: Mus musculus

text: thymus

text: RIKEN full-length enriched, 10 days neonate thymus


text: live

text: Generic

text: Cabernet Sauvignon

text: CA41LU is a cDNA library of Cabernet Sauvignon leaves. The leaves were collected on September 20, 2001, in Napa Valley, California, and represent leaves in late season development. These leaves were asymptomatic and were verified to be non-infected with the bacterial pathogen, Xylella fastidiosa, based on a diagnostic assay using PCR and Xylella-specific primer pairs.cDNA made by oligo-dT priming and directionally cloned. 5'and 3' adaptors were used in cloning as follows: 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' and 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)NN-3'. Library was constructed using the Clontech Creator SMART kit and size-selected to contain the 0.5-3 kb size fraction.

characteristic: description

text: Late season sample

text: Leaf

text: Vitis vinifera

text: SfiI

text: SfiI

text: hermaphrodite

text: Cabernet Sauvignon Leaf - CA41LU

text: Plasmid

text: pDNR


text: live

text: Generic

text: Cabernet Sauvignon

text: CA48LU is a cDNA library of Cabernet Sauvignon leaves. The leaves were collected on September 20, 2001, in Napa Valley, California, and represent leaves in late season development. These leaves were asymptomatic and verified to be non-infected with the bacterial pathogen, Xylella fastidiosa, based on a diagnostic assay using PCR and Xylella-specific primer pairs. cDNAs were made by oligo-dT priming and directionally cloned. 5'and 3' adaptors were used in cloning as follows: 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' and 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)NN-3'. Library was constructed using the Clontech Creator SMART kit and size-selected to contain the 0.5-3 kb size fraction.

text: Late season sample

characteristic: lab-host

text: Leaf

text: Vitis vinifera

text: SfiI

text: SfiI

text: hermaphrodite

text: Cabernet Sauvignon Leaf - CA48LU

text: Plasmid

text: pDNR


text: live

text: Generic

text: 10 days embryo

text: Mus musculus

text: whole body

text: RIKEN full-length enriched, 10 days embryo whole body


text: live

text: Generic

text: 10, 11 days embryo

text: Mus musculus

text: whole body

text: RIKEN full-length enriched, 10, 11 days embryo whole body


text: live

text: Generic

text: 11 days embryo

text: Mus musculus

text: whole body

text: RIKEN full-length enriched, 11 days embryo whole body


text: live

text: Generic

text: 10 days neonate

text: Mus musculus

text: skin

text: RIKEN full-length enriched, 10 days neonate skin


text: live

text: Generic

text: sporozoite

text: Plasmodium falciparum

text: P. falciparum NF54 sporozoite cDNA library


text: live

text: Generic

text: Source parasite material isolated by laser capture microdissection

text: Liver Stage

text: Plasmodium yoelii

text: 17X NL

text: P. yoelii 17X NL LCM liver-stage cDNA library


text: live

text: Generic

text: The subtracted PCR products were cloned into Pinpoint Xa-1 T-vector after suppression subtractive hybridization with dealate-mating queen as 'tester' and alate-virgin queen as 'driver'.

text: Solenopsis invicta

text: Solenopsis invicta subtracted cDNA library


text: live

text: Generic

text: artificial construct

characteristic: cell type

text: Rattus norvegicus


text: live

text: Generic

text: Rattus norvegicus


text: live

text: Generic

text: Library is oligo-dT primed and directionally cloned (EcoRV site is destroyed upon cloning). Average insert size 1.68. Library was constructed by (Invitrogen).

text: DH10B-Ton A ( T1 and T5 phage resistances)

text: Pooled gut

text: Homo sapiens

text: NotI

text: EcoRV (destroyed)

text: Stomach, small intestine, colon, esophagus and gall bladder

characteristic: tissue-type

text: plasmid (ampicillin resistant)


text: live

text: Generic

text: Bradfield Lab RACE method Note: this is a NIH_MGC Library

text: DH10B-Ton A( T1 and T5 phage resistances)

text: Homo sapiens

text: PacI

text: AscI

text: Embryonic Stem Cells H1

text: plasmid (ampicillin resistant)

text: pCI-neo

characteristic: vector


characteristic: External Id

text: live

text: Generic

text: 0-24 hours mixed stage embryos

text: DH5-alpha TonA

text: embryo

text: Drosophila melanogaster

text: male and female

characteristic: sex

text: RE Drosophila melangaster normalized Embryo pFlc-1


text: live

text: Generic

text: In the suppression subtractive hybridization, the fire ant dealate queen was used as 'tester', alate queen was used as 'driver'.

text: adult, queen

text: whole body

text: Solenopsis invicta

characteristic: organism

text: female

text: Solenopsis invicta suppression subtractive hybridization library

characteristic: title

text: Pinpoint Xa-1 T-vector


characteristic: INSDC first public

text: live

text: Generic

text: Funding: The production of ESTs submitted in this project was funded by USDA Grant MRI-2002-03476 entitled 'Bovine ESTs: Focus on Female Reproduction' to RS Prather, E Antoniou, HA Garverick, JA Green, MC Lucy, RM Roberts, MF Smith and RS Youngquist. Genetic Source: Heifers for the project were purchased from Circle A Ranch, Iberia, MO (http://www.circlearanch.com/home.html). These heifers, while not registered have known Angus pedigrees going back at least 4 generations. Samples collected: The samples consisted of the following: germinal vesicle-stage oocytes; in vitro derived embryos (2-cell, morula, blastocyst and nuclear transfer blastocyst); in vivo blastocysts and conceptuses (days 8, 14, 16 and 18); corpora lutea (days 3, 5, 8, 14, 16, 18 and 35); ovarian follicles (days 0, non-recruited, recruited, early selected and preovulatory); oviduct (days 0, 3 and 5); endometrium (days 5, 8, 14, 16, 18 and 35); and placenta/embryo from day 35 conceptuses. Expanded descriptions of how the tissues were collected can be found at the following URL: http://genome.rnet.missouri.edu/Bovine/Methods.html. Library construction (Standard Protocol): All procedures have been described in detail elsewhere (Soares et al., 1994; Bonaldo et al., 1996; Jiang et al., 2001). Total cellular RNA from each sample was isolated by using STAT-60 reagent (Tel-Test, Friendswood, TX) and the poly(A)+ RNA was obtained by two rounds of purification with the Oligotex mRNA isolation kit (Qiagen) according to the manufacturer's instructions. The libraries were constructed essentially as described by the manufacturer's instructions provided with the SuperScript Plasmid System (Invitrogen, cat. no. 18248-013). Briefly, 1mcg of poly(A)+ RNA was annealed at c37 degrees with 10mcg of NotI-tag-dT18 oligonucleotide (GCTGCTCGCGGCCGC-tag-T18) and reverse transcribed at c37 degrees with SuperScript II (Invitrogen) reverse transcriptase (Jiang et al., 2001). The 'tag' represents a tissue/stage-specific ten-base sequence identifier (http://genome.uiowa.edu/pubsoft/software.html) present in the oligonucleotide used to prime first-strand synthesis. Second strand synthesis was performed with T4 DNA polymerase in the presence of DNA ligase and RNase H. After second strand synthesis, the double-stranded cDNAs were ligated to SalI adapters (Invitrogen-Life Technologies) and digested with NotI. The cDNAs were size selected by passage through cDNA size fractionation columns (Invitrogen-Life technologies). The cDNAs derived from each developmental stage of a particular tissue were mixed on an equimolar basis and ligated directionally into the NotI and SalI sites of the pCMV-SPORT6 vector (Invitrogen). After ligation of the inserts, the plasmids were electroporated into DH10B bacteria. Library Construction (PCR Protocol): The amount of mRNA that was recovered from oocytes and embryos was quite limiting and was not sufficient for library production with the standard protocol. Therefore, PCR-based protocol was utilized for producing libraries from sources in which the amount of extracted mRNA was small (oocytes and embryos). Poly-A RNA was isolated by using the MicroPoly(A) Pure kit from Ambion (cat. # 1918). The mRNA was reverse transcribed with a NotI-tag-dT18 oligonucleotide and a SMART oligonucleotide (Clontech) modified to contain a SalI site to generate full-length cDNA with a sequence complementary to the SMART oligonucleotide. Sequences within the SMART and dT oligonucleotides were used as primers to amplify the cDNAs by PCR with pfu turbo polymerase (Stratagene). The resulting PCR products were purified, digested with NotI and SalI and size fractionated by using Chroma Spin-1000 columns (Clontech). Purified cDNA from each PCR reaction was quantitated and mixed on an equimolar basis for ligation into the pCMV- SPORT6 vector. Preliminary Library Characterization: Randomly chosen clones from each library were analyzed by restriction digestion to determine average insert size (96 clones) and by sequencing (~4 96-well plates) to confirm library quality [e.g. the presence of short polyA+ tails, genomic DNA contamination (must be <1%), ribosomal RNA clones (must be <1%), etc.] and to provide a sequence database representing the predominant clones in each library. The clones were sequenced at the University of Missouri-Columbia DNA Core Facility. After production of the libraries, equal numbers of recombinants from each library were pooled to produce a single mixed library (mega-library) for more extensive sequencing. Bioinformatics work was performed by GK Springer's bioinformatics group in Computer Science at the University of Missouri-Columbia. Clone Requests: Requests for clones should be made to the Director of the University of Missouri DNA Core facility at: bovine@rnet.missouri.edu. Bonaldo MF, Lennon G, Soares MB,. Normalization and Subtraction: Two approaches to facilitate gene discovery. Genome Res, 1996; 6:791-806. Jiang H, Bivens NJ, Ries JE, Whitworth KM, Green JA, Forrester LJ, Springer GK, Didion BA, Mathialagan N, Prather RS, Lucy MC (2001) Constructing cDNA libraries with fewer clones that contain long poly(dA) tails. Biotechniques 31:38-42. Soares MB, MF Banaldo, P Jelene, L Su, L Lawton, A Efstrantiadis. 1994. Construction and characterization of a normalized cDNA library. Proc Natl Acad Sci, 91:9228-9232.

text: Bos taurus


text: live

text: Generic

characteristic: NCBI submission package

text: Funding: The production of ESTs submitted in this project was funded by USDA Grant MRI-2002-03476 entitled 'Bovine ESTs: Focus on Female Reproduction' to RS Prather, E Antoniou, HA Garverick, JA Green, MC Lucy, RM Roberts, MF Smith and RS Youngquist. Genetic Source: Heifers for the project were purchased from Circle A Ranch, Iberia, MO (http://www.circlearanch.com/home.html). These heifers, while not registered have known Angus pedigrees going back at least 4 generations. Samples collected: The samples consisted of the following: germinal vesicle-stage oocytes; in vitro derived embryos (2-cell, morula, blastocyst and nuclear transfer blastocyst); in vivo blastocysts and conceptuses (days 8, 14, 16 and 18); corpora lutea (days 3, 5, 8, 14, 16, 18 and 35); ovarian follicles (days 0, non-recruited, recruited, early selected and preovulatory); oviduct (days 0, 3 and 5); endometrium (days 5, 8, 14, 16, 18 and 35); and placenta/embryo from day 35 conceptuses. Expanded descriptions of how the tissues were collected can be found at the following URL: http://genome.rnet.missouri.edu/Bovine/Methods.html. Library construction (Standard Protocol): All procedures have been described in detail elsewhere (Soares et al., 1994; Bonaldo et al., 1996; Jiang et al., 2001). Total cellular RNA from each sample was isolated by using STAT-60 reagent (Tel-Test, Friendswood, TX) and the poly(A)+ RNA was obtained by two rounds of purification with the Oligotex mRNA isolation kit (Qiagen) according to the manufacturer's instructions. The libraries were constructed essentially as described by the manufacturer's instructions provided with the SuperScript Plasmid System (Invitrogen, cat. no. 18248-013). Briefly, 1mcg of poly(A)+ RNA was annealed at c37 degrees with 10mcg of NotI-tag-dT18 oligonucleotide (GCTGCTCGCGGCCGC-tag-T18) and reverse transcribed at c37 degrees with SuperScript II (Invitrogen) reverse transcriptase (Jiang et al., 2001). The 'tag' represents a tissue/stage-specific ten-base sequence identifier (http://genome.uiowa.edu/pubsoft/software.html) present in the oligonucleotide used to prime first-strand synthesis. Second strand synthesis was performed with T4 DNA polymerase in the presence of DNA ligase and RNase H. After second strand synthesis, the double-stranded cDNAs were ligated to SalI adapters (Invitrogen-Life Technologies) and digested with NotI. The cDNAs were size selected by passage through cDNA size fractionation columns (Invitrogen-Life technologies). The cDNAs derived from each developmental stage of a particular tissue were mixed on an equimolar basis and ligated directionally into the NotI and SalI sites of the pCMV-SPORT6 vector (Invitrogen). After ligation of the inserts, the plasmids were electroporated into DH10B bacteria. University of Missouri DNA Core facility at: bovine@rnet.missouri.edu. Bonaldo MF, Lennon G, Soares MB,. Normalization and Subtraction: Two approaches to facilitate gene discovery. Genome Res, 1996; 6:791-806. Jiang H, Bivens NJ, Ries JE, Whitworth KM, Green JA, Forrester LJ, Springer GK, Didion BA, Mathialagan N, Prather RS, Lucy MC (2001) Constructing cDNA libraries with fewer clones that contain long poly(dA) tails. Biotechniques 31:38-42. Soares MB, MF Banaldo, P Jelene, L Su, L Lawton, A Efstrantiadis. 1994. Construction and characterization of a normalized cDNA library. Proc Natl Acad Sci, 91:9228-9232.

text: Bos taurus


characteristic: INSDC last update

text: live

text: Generic

text: First-strand cDNA was primed with the oligo-dT primer 5'-AATTCGAGCGGCCGCT(30)VN-3' and a switch primer 5'-TACGGCTGCGAGAAGACGACAGAAGGG-3'. DNA was amplified by 26 cycles of PCR and then size selected for >0.5 kb. An aliquot was used to reamplify for 7 more cycles. The BstXI adaptor 5'-TACGGCTGCGAGAAGACGACAGAAGGG-3' was ligated at both ends and non-directionally cloned into the BstXI site of the pcDNAIII vector. Original library contained 7.5 million clones. Library constructed by Mike Clarke (University of Michigan). Note: this is a NIH_MGC library.

text: stem cell

characteristic: organ

text: Mus musculus

text: BstXI

text: BstXI

text: early hematopoietic precursor cells, isolated from bone marrow

text: phagemid

text: pCDNAIII


text: live

characteristic: INSDC status

text: Generic

text: Smooth Cayenne

characteristic: cultivar

text: Ripe yellow fruit

text: Ananas comosus

text: Fruit flesh

text: Pineapple ripe yellow fruit cDNA library


text: live

text: Generic

characteristic: NCBI submission model

text: Fantasia

text: S4 climacteric

text: Prunus persica

text: mesocarp

text: Prunus persica mesocarp


text: live

text: Generic

text: Oligo-dT primed reverse transcription optimized for large and GC rich mRNA transcripts, cDNA size selection, optimized ligation for large inserts into mammalian expression vector, random clones selected for end sequence verification of full-length genes; constructed and available from OriGene Technologies

text: Homo sapiens

text: Xho1/Sal1 compatible end ligatio

text: Skeletal muscle

text: Human skeletal muscle, large insert, pCMV expression library

text: Plasmid


text: live

text: Generic

text: Seeds were surface sterilized with 0.1% (w/v) sodium hypo chlorite solution for 5 min, thoroughly rinsed with distilled water and germinated in plastic pots contained soil and sand (2:1) mixture allowed to grow for fourteen days. The pots were maintained in the departmental botanic garden under natural photoperiod (10-12 hrs, 28 ( 4oC). Seedlings were maintained at 100% field capacity by watering daily. Fourteen day-old plants were subjected to moisture stress by withholding water. Leaf samples collected from unstressed (100% FC) and stressed (once field capacity reached 25%) plants, flash frozen and stored at -80oC. The frozen samples were used for RNA extraction (LiCl2 method). PolyA mRNA was isolated using streptavidin paramagnetic particles (SPMPs; Roche, Germany). First-strand cDNA synthesis was initiated from the enriched mRNA of driver (unstressed) using biotin labeled poly dT and Superscript RNaseH- reverse transcriptase (Gibco-BRL, USA). Subtractive hybridization was performed between biotin labeled driver ss cDNA(unstressed) and tester (stressed) mRNA at 65oC for 1 hour. The tester-driver hybrids and excess of the driver were separated from the unhybridized tester mRNA using streptavidin paramagnetic particles (SPMPs; Roche, Germany). Tester mRNAs that did not hybridize with the driver were used for cDNA synthesis (Stratagene). A primary cDNA library was produced using a lambda ZAP XR cDNA Synthesis Kit (Stratagene). Randomly selected clones amplified using M13F and M13R-primers, PCR product was purified using PCR Purification system (Stratagene) and partially sequenced from the 3' end on an MegaBACE 1000 DNA analysis system (Amersham Pharmacia, Uppsala, Sweden). Sequences are processed using the HarvEST pipeline (http://harvest.ucr.edu) to remove vector and cloning oligo sequences and various contaminants, and to trim to a high quality region. Sequences that retained at least 100 bases were deposited to GenBank. Sequences that retained at least 100 bases were deposited to GenBank.

text: 24 days seedlings

characteristic: dev-stage

text: Ecoli

text: Macrotyloma uniflorum

text: EcoRI

text: XhoI

text: Leaf

text: Horsegram (VZM-1) drought stressed subtracted cDNA 24 days seedlings

text: Phagemid pBluescript SK(-)

text: Lambda Uni-ZAP XR


text: live

text: Generic

text: 10 days old

text: Mus musculus

text: male

characteristic: strain

text: pancreas

text: RIKEN full-length enriched mouse cDNA library, C57BL/6J pancreas male 10 days old


text: live

text: Generic

text: germinal center B cells

characteristic: cell-line

text: Constructed from size fractionated cytoplasmic mRNA (4.4-7.4kb). Directionally cloned. Cells provided by Louis M. Staudt, Ph.D. Library preparation by Maria de Fatima Bonaldo, Ph.D. and M. Bento Soares, Ph.D.

characteristic: description

text: DH10B (LTI)

characteristic: lab-host

text: Homo sapiens

text: NotI

text: Eco RI

text: lymph

text: plasmid


text: live

text: Generic

text: germinal center B cells

characteristic: cell type

text: Constructed from size fractionated cytoplasmic mRNA (7.4-9.5kb). Directionally cloned. Cells provided by Louis M. Staudt, Ph.D. Library preparation by Maria de Fatima Bonaldo, Ph.D. and M. Bento Soares, Ph.D.

text: DH10B (LTI)

text: Homo sapiens

text: NotI

text: Eco RI

text: lymph

characteristic: tissue-type

text: plasmid

characteristic: vector


characteristic: External Id

text: live

text: Generic

text: germinal center B cells

text: Constructed from size fractionated cytoplasmic mRNA (3.5-4.4kb). Directionally cloned. Cells provided by Louis M. Staudt, Ph.D. Library preparation by Maria de Fatima Bonaldo, Ph.D. and M. Bento Soares, Ph.D.

text: DH10B (LTI)

text: Homo sapiens

characteristic: organism

text: NotI

text: Eco RI

text: lymph

text: plasmid


characteristic: INSDC first public

text: live

text: Generic

text: mycelium growth induced by chitin

text: Arcopilus cupreus

text: mycelium

text: Chaetomium cupreum mycelium cDNA induced by chitin

characteristic: title


characteristic: INSDC last update

text: live

text: Generic

characteristic: NCBI submission package

text: Solid-cultured basidiom of A. cinnamomea obtained from Division of Forest Protectin of Taiwan Forestry Research Institute. The cDNA library was constructed by pGEM-T Easy vector system (Promega).

text: Solid-cultured

text: Taiwanofungus camphoratus

text: basidiom

text: Antrodia cinnamomea cDNA library

text: pGEM-T Easy


text: live

characteristic: INSDC status

text: Generic

text: Human Primary Fibroblasts

text: GM05387, Coriell Institute for Medical Research, Camden, NJ

text: AAV Vector:Chromosome Junction Sequences rescued as plasmids in bacteria. Files with names differing only by x1 or y1 contain sequence reads from either end of the same AAV vector provirus. 'y1' indicates the 'left' and 'x1' indicates the 'right' primer was used to initiate sequencing reactions.

text: Homo sapiens

text: male

characteristic: sex

text: Adeno-Associated Virus Vector Integration Junctions


text: live

text: Generic

characteristic: NCBI submission model

text: ded, double-stranded cDNA. The EcoRI-XhoI-digested cDNA was ligated with EcoRI-XhoI-digested pBluescript KS+ (Invitrogen Corp).

text: E. coli

text: Phanerodontia chrysosporium

text: XhoI

text: EcoRI

text: Phanerochaete chrysosporium pBluescript (EcoRI-XhoI).

text: plasmid

text: pBluescript KS+


text: live

text: Generic

text: Lactating buffalo mammary gland tissue was processed for RNA isolation to create a directionally cloned cDNA library in pDNR-LIB vector

text: lactating stage

characteristic: dev-stage

text: E.coli DH5 alpha

text: Bubalus bubalis

text: SfiI

text: SfiI

text: Female

text: Murrah

text: Mammary gland

text: cDNA library from Lactating buffalo mammary gland

text: Plasmid

text: pDNR-LIB


text: live

text: Generic

text: Total and poly A+ RNA was isolated from the indicated stickleback tissue, and a cDNA library was constructed in the Express 1 plasmid vector by Open Biosystems. First strand cDNA synthesis was primed with an 54 bp linker primer containing an oligodT sequence preceeded by a synthetic NotI site (first strand primer: 5'-GACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3'). Following second strand synthesis, cDNAs were made blunt at the end corresponding to the original 5 prime end of mRNA, and cloned directionally into the NotI and EcoRV sites of Express 1. Note that the EcoRV site is typically destroyed in the blunt end cloning, leaving a junction of the form xxxATC(where is ATC is the second half of the EcoRV site, and xxx is derived from the cDNA sequence). A map of the Express 1 vector is available at: http://www.openbiosystems.com/cdna_library_construction_faq.php#8 The primary library was transformed and amplified in DH10B (T1 phage resistant) bacteria.

text: 21 day old larvae collected at Swarup Stage 30 (J. Embryol. Exp. Morphol 6: 373-383.1958)

text: DH10B (T1 phage resistant)

text: Gasterosteus aculeatus

text: mixed male and female

text: Bitrufjordur marine sticklebacks, Iceland

characteristic: strain

text: whole larva

text: Plasmid

text: Express 1


text: University of Padova

characteristic: INSDC center name

text: live

text: Generic

text: Golden Delicious

characteristic: cultivar

text: E. coli DH5alpha

text: Malus domestica

text: abscission zones

text: malus abscission express library

text: plasmid

text: pGEM-T Easy


text: Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences

text: live

text: Generic

text: Musa acuminata

text: Musa acuminata library (Feng R)


text: Washington University School of Medicine

text: live

text: Generic

text: Adult

characteristic: lab-host

text: Petromyzon marinus

text: Sfi I

text: Sfi I

text: Mix

text: EST

text: pDNR-LIB


text: live

text: Generic

text: Geneva 3041

text: Total RNA was extracted separately from whole root using the 'pin tree' method(Gasic et.al,2004). Poly(A)+mRNA was isolated twice from total RNA from each stage using the Oligotex Direct mRNA kit(Qiagen). MRNA was reverse transcribed into double stranded cDNA using a modified oligo18(dT) primer with an identifying tag sequence. [Tag identification when sequencing from 5' end: insert 18(A)TGCTGG}]. Double stranded cDNAs were size selected(more than 500bp), adaptored with EcoRI adapters at both ends and then digested with NotI. The cDNAs were then directionally cloned into EcoRI-NotI digested pBS II SK+ phagemid vector (Stratagent). The total number of white colony forming units was 1.2x10^6 cfu. The background of empty clones was less than 10%. Inserts ranged from 0.5kb to 3kb as determined by PCR.

characteristic: description

text: Tissue challenged with Erwinia

text: DH10B cells

text: Malus hybrid rootstock

text: Not I

text: EcoRI

text: Phagemid

text: pBluescript II SK (+)


text: live

text: Generic

text: Granny

text: Total RNA was extracted from freeze dried fruit tissue, using the method described by Wang and Vodkin(Plant Molecular Biology Reporter 12:132-145, 1994). Poly(A)+mRNA was isolated from total RNA using the PolyATtract mRNA Isolation system III(Promega). The library was prepared using the Stratagene pBluescript II XR cDNA library construction kit. Complementary DNA was synthesized from mRNA using a Poly(dT)sequence with a Xho-I restriction site. EcoRI adapters were ligated to the blunt ended cDNA fragments followed by restriction with XhoI. The cDNA insert is protected from XhoI digestion via methylation during first strand cDNA synthesis. The cDNA fragments were directionally cloned into the EcoRI-XhoI restriction site of the pBluescript vector. The ligated cDNA fragments were transformed into E.coli ElectroMax DH10B host cells.

text: 35 days after pollination

text: DH10B cells

text: Malus domestica

text: XhoI

text: EcoRI

text: Fruit tissue

characteristic: tissue-type

text: Phagemid

text: pBluescript II SK (+)


text: live

text: Generic

text: Suncrisp

text: 35 days after pollination

text: DH10B cells

text: Malus domestica

text: XhoI

text: EcoRI

text: Fruit tissue

text: Phagemid

text: pBluescript II SK (+)

characteristic: vector


characteristic: External Id

text: live

text: Generic

text: Braeburn

text: 35 days after pollination

text: DH10B cells

text: Malus domestica

text: XhoI

text: EcoRI

text: Fruit tissue

text: Phagemid

text: pBluescript II SK (+)


text: Instituto Valenciano de Investigaciones Agrarias (IVIA)

text: live

text: Generic

text: adult

text: Citrus sinensis

characteristic: organism

text: hermaphrodite

text: Washington Navel

text: Mature fruit abscission zone C

text: AbsCFruit

text: Plasmid


characteristic: INSDC first public

text: live

text: Generic

text: Roots subjected to nutritional and toxic chloride treatments

text: adult

text: Citrus reshni

text: hermaphrodite

text: cleopatra

text: roots

text: Plasmid


text: Vrije Universiteit Amsterdam

text: live

text: Generic

text: supplier: Petunia x hybrida cv. R27 Diploid plants. RNA was extracted from petal of buds at maximum elongation.

text: Petunia x hybrida

text: petals

text: petunia corolla cDNA library

characteristic: title


text: National Institute of Agricultural Biotechnology

characteristic: INSDC last update

text: live

text: Generic

text: Daucus carota

text: Daucus carota cDNA library


text: The University of Arizona

text: live

text: Generic

characteristic: NCBI submission package

text: Orange Turmeric

text: Greenhouse, soil grown

text: TJC 121

text: Curcuma longa

text: EcoRI

text: XhoI

text: young leaves

text: Plasmid

text: pBluescriptIISK-


text: The University of Arizona

text: live

characteristic: INSDC status

text: Generic

text: Orange Turmeric

text: Greenhouse, soil grown

text: TJC 121

text: Curcuma longa

text: EcoRI

text: XhoI

text: rhizome

text: Plasmid

text: pBluescriptIISK-


text: The University of Arizona

text: live

text: Generic

text: young leaf

text: TJC 121

text: Ocimum basilicum

text: EcoRI

text: XhoI

text: peltate glandular trichome

text: Plasmid

text: pBluescriptSK-


text: The University of Arizona

text: live

text: Generic

text: MC, cinnamon basil

text: young leaf

text: TJC 121

text: Ocimum basilicum

text: EcoRI

text: XhoI

text: peltate glandular trichome

text: Plasmid

text: pBluescriptSK-


text: The University of Arizona

text: live

text: Generic

text: Sweet Dani, SD

text: young leaf

text: TJC 121

text: Ocimum basilicum

text: EcoRI

text: XhoI

text: peltate glandular trichome

text: Plasmid

text: pBluescriptSK-


text: The University of Arizona

text: live

text: Generic

text: SW

text: young leaf

text: TJC 121

text: Ocimum basilicum

text: EcoRI

text: XhoI

text: Peltate glandular Trichome

text: pBluescriptIISK-


text: The University of Arizona

text: live

text: Generic

text: Yellow Ginger

text: Greenhouse, soil grown

text: TJC 121

text: Zingiber officinale

text: EcoRI

text: XhoI

text: young leaf

text: Plasmid

text: pBluescriptIISK-


text: The University of Arizona

text: live

text: Generic

text: White Ginger

text: Greenhouse, soil grown

text: TJC 121

text: Zingiber officinale

text: EcoRI

text: XhoI

text: rhizome

text: Plasmid

text: pBluescriptIISK-


text: The University of Arizona

text: live

text: Generic

text: Yellow Ginger

text: Greenhouse, soil grown

text: TJC 121

text: Zingiber officinale

text: EcoRI

text: XhoI

text: rhizome

text: Plasmid

text: pBluescriptIISK-


text: The University of Arizona

text: live

text: Generic

text: White Ginger

text: Greenhouse, soil grown

text: TJC 121

text: Zingiber officinale

text: EcoRI

text: XhoI

text: root

text: Plasmid

text: pBluescriptIISK-


text: The University of Arizona

text: live

text: Generic

text: Yellow Ginger

text: Greenhouse, soil grown

text: TJC 121

text: Zingiber officinale

text: EcoRI

text: XhoI

text: root

text: Plasmid

text: pBluescriptIISK-


text: The University of Arizona

text: live

text: Generic

text: White Ginger

text: Greenhouse, soil grown

text: TJC 121

text: Zingiber officinale

text: EcoRI

text: XhoI

text: young leaf

text: Plasmid

text: pBluescriptSK(-)


text: RMIT University

text: live

text: Generic

text: Prepared with Clontech SMART PCR cDNA Synthesis kit, pGEM-T Easy vector, E. coli JM109, QIAGEN mini-prep kit, Big-Dye sequencing

text: 2 weeks and 48-72 hours post-inoculation with Mycosphaerella pinodes

text: Lathyrus sativus

text: Stem and leaf

text: Grasspea (ATC80878) post-inoculation EST library


text: Iowa State University

text: live

text: Generic

text: Zea mays

text: UGIII-Reseq


text: Iowa State University

text: live

text: Generic

text: Zea mays


text: Iowa State University

text: live

text: Generic

text: Zea mays

text: UGV-Reseq


text: Auburn University, Cellular and Molecular Biosciences Program

text: live

text: Generic

text: Msx1 cDNA amplified from reverse transcribed endometrial RNA

text: pregnancy day 12

text: uterus

characteristic: organ

text: Sus scrofa

text: female

characteristic: sex

text: endometrium

text: porcine endometrium

text: pDrive


text: Biotechnology Research Institute, National Research Council of Canada

text: live

text: Generic

characteristic: NCBI submission model

text: A suppressive subtractive library was made from purified larval midgut mRNA. Digested fragments (Not I and Eag I) were cloned into the QuanTox vector. The library consists of both forward and reverse libraries.

characteristic: dev-stage

text: Midgut

text: Choristoneura fumiferana

text: (Clemens)

text: C. fumiferana midgut suppressive subtractive library

text: Plasmid

text: QuanTox


text: Korea University

characteristic: INSDC center name

text: live

text: Generic

text: Karl (Clho 15487)

text: Total RNA was extracted from the grains of the 14 DAF barley.

text: Hordeum vulgare subsp. vulgare

text: kernel (without lemma and pelea)

text: cDNA library of barley kernel of 14 days after fertilization (14 DAF)


text: USDA, ARS, US Meat Animal Research Center

text: live

text: Generic

text: ulb, and pineal gland.

text: Bos taurus

text: EcoRI

text: XhoI

text: pooled

text: MARC 11BOV

text: plasmid

text: pDNR-LIB


text: Texas A&M University

text: live

text: Generic

text: Texas Maker-1 (TM-1)

characteristic: cultivar

text: G. hirsutum L. Texas Marker-1 (TM-1) plants were grown in a greenhouse at the Texas A&M University and in the field at the USDA-ARS, New Orleans. The bolls were tagged and harvested at 3 days before anthesis (-3DPA), the day of anthesis (0 DPA), and 3 days post anthesis (3DPA) from a pool of ~20 plants. Total RNA was extracted from immature ovules or fiber-bearing ovules. An equal amount of total RNA from three samples was mixed and sent to Invitrogen Corp. (Carsbad, California) for full-length cDNA library construction. Messenger RNA was isolated from the total RNA using a filter syringe containing oligo (dT). The first-strand cDNA was synthesized from 15 ug mRNA using SuperScriptTMIII reverse transcriptase, and the second-strand cDNA was synthesized using E. coli RNase H, DNA polymerase I, and DNA ligase. The double-stranded cDNA was blunt-ended using T4 DNA polymerase, digested with NotI, size-selected using agarose gel electrophoresis, and directionally cloned into the NotI-EcoRV sites of the pCMV-SPORT6.1. The ligated products were transformed using ELECTROMAXTMDH10BT1 cells. The library contained 4.2x106 cfu with ~100% of colony growth rate. It is estimated that over 60% clones contained full-length cDNA inserts with an average insert size of 1.53-kb. A total of 51,072 colonies were arrayed in duplicate sets each with 133 384-well plates for sequencing and storage, respectively.

text: Immature ovules (-3 to 3 days post anthesis, DPA) with or without fibers

text: Gossypium hirsutum

text: EcoRI

text: NotI

text: Immature ovules


text: Institute for Biochemistry, Faculty of Medicine

text: live

text: Generic

text: generated tissue. By doing this we harvested up-regulated genes in regenerating tissue. Subtracted library of up-regulated genes was cloned into pCRII plasmids using TA cloning kit (Invitrogen).All the obtained clones were sequenced.

text: One Shot Competent Cells

characteristic: lab-host

text: limb

text: Ambystoma mexicanum

text: male

text: wild type

characteristic: strain

text: Axolotl blastema subtracted library, day 4

text: Plasmid

text: pCRII


text: USDA-ARS; Jamie Whitten Delta States Research Center

text: live

text: Generic

text: A high quality EST with at least 200 contiguous bases at Trace Tuner score of 20 or better. Library construction by Margaret Allen, USDA,ARS, National Biological Control Laboratory, Stoneville, MS, USA.

characteristic: description

text: nymph

text: Lygus lineolaris

text: Male

text: whole body

characteristic: tissue-type

text: Male nymph TPB


characteristic: External Id

text: College of Veterinary Medicine, China Agricultural University

text: live

text: Generic

text: unsporulated oocyst, sporulated oocyst, sporozoite, merozoite

text: Eimeria tenella

text: Normalized Eimeria tenella cDNA library

text: pBluescript SK(+)

characteristic: vector


text: Medical University of South Carolina

characteristic: INSDC first public

text: live

text: Generic

text: PBL

characteristic: cell type

text: Tursiops truncatus

characteristic: organism

text: GAATTC

text: PBL

text: Tursiops truncatus EST Library (L99-05) at marinegenomics.org

text: Plasmid


text: University of Edinburgh

characteristic: INSDC last update

text: live

text: Generic

characteristic: NCBI submission package

text: The library was constructed by Mark Blaxter in Edinburgh from double stranded cDNA from hydrated rotifers provided by Dr. Natalia Pouchkina-Stancheva of the Institute of Biotechnology, Cambridge.

text: adult

text: Philodina roseola

text: female

text: Philodina roseola hydrated library

characteristic: title

text: pCR-XL-TOPO


text: The Natural History Museum

text: live

characteristic: INSDC status

text: Generic

text: SSH cDNA library, enriched for differentially expressed genes, was sequenced using M13F. Adaptor primer sequences were removed from sequence.

text: Biomphalaria glabrata

text: Hermaphrodite

characteristic: sex

text: NHM 3017/NHM 1742

text: Haemocytes/Haemopoietic organ

text: BgSSH

text: pGEM


text: Hunan Agricultural University, China

text: live

text: Generic

characteristic: NCBI submission model

text: Growing stage

characteristic: dev-stage

text: Camellia sinensis

text: leaf

text: Anjibai Tea Whited Leaf Express Library

text: pGEM-T Easy


text: Hunan Agricultural University, China

characteristic: INSDC center name

text: live

text: Generic

text: Growing stage

text: Camellia sinensis

text: leaf

text: Anjibai Tea Green Leaf Express Library

text: pGEM-T Easy


text: Washington University School of Medicine

text: live

text: Generic

text: Fertilized egg to Completion of digestive tract

characteristic: lab-host

text: Petromyzon marinus

text: Sfi I

text: Sfi I

text: Mix

text: EST

text: pDNR-LIB


text: Northwest A&F University

text: live

text: Generic

text: young spike

text: DNA sequencing kit-LC, sequences were achieved using LiCOR-4200 DNA Sequencer.

text: dinucleate stage

text: Triticum aestivum

text: spike

text: SSH-cDNA Library of young spike of YS type under controlled male fertile condition


text: Jiangsu Academy of Agricultural Sciences

text: live

text: Generic

text: the mixed mRNA from seedlings treated 24h, 48h and 72h by 200 mM NaCl as Tester and the non- NaCl treated as 'Driver'.

text: seedling

text: Salicornia bigelovii

text: EcoRI

text: NotI

text: whole plant

text: Salicornia bigelovii Torr. cDNA library based on suppression subtractive hybridization

text: pGEM-T


text: UMR PAVE-77, Universite d'Angers, Laboratoire de Mycologie

text: live

text: Generic

text: A 2-day-old germinating conidia grown in potato dextrose broth were treated with Allyl isothiocyanate at a concentration equivalent to IC50 (2.5 mM) for 30 minutes

text: Conidia

text: Alternaria brassicicola

characteristic: strain

text: A. brassicicola conidia exposed to Allyl isothiocyanate

text: pGEMT


text: Biocenter Klein Flottbek, University of Hamburg

text: live

text: Generic

text: zygote

text: inbred A188

characteristic: cultivar

text: 18 h after in vitro fertilization

text: Zea mays

text: ZmZ


text: live

text: Generic

text: pollen

text: inbred A188

text: mature

text: Zea mays

text: ZmP


text: University of Sao Paulo

text: live

text: Generic

text: AGAAACCG-3'. Fragments cloned into p-GEM T-Easy vector System (Promega). Sequence obtained using M13 forward primer.

characteristic: description

text: adult

text: brain

characteristic: organ

text: Mus musculus

text: mouse brain total RNA

text: plasmid

text: p-GEM T-Easy


characteristic: External Id

text: live

text: Generic

text: A 2-day-old germinating conidia grown in potato dextrose broth were treated with camalexin at a concentration equivalent to IC50 (125 µM) for 30 minutes

text: Conidia

text: Alternaria brassicicola

text: A. brassicicola conidia exposed to camalexin

text: pGEMT

characteristic: vector


text: Northwest A&F University

characteristic: INSDC first public

text: live

text: Generic

text: young spike

characteristic: cell type

text: I DNA sequencing kit-LC, sequences were achieved using LiCOR-4200 DNA Sequencer.

text: dinucleate stage

text: Triticum aestivum

text: spike

characteristic: tissue-type

text: SSH-cDNA Library of young spike of YS type under controlled male sterile condition


text: National Cancer Institute / NIH

characteristic: INSDC last update

text: live

text: Generic

characteristic: NCBI submission package

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer 5'-GACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3', double-stranded cDNA was cloned into the NotI and EcoRV sites of pExpress-1 (EcoRV site destroyed upon ligation). Library was size-selected for for an average insert size of 1.45 kb. Primary library, non-amplified. Use M13(-21) to generate 3' end reads and M13R to generate 5' end reads. Library was constructed by Open Biosystems (Huntsville, AL). Note: this is a (http://zgc.nci.nih.gov/) Zebrafish Gene Collection library.

text: DH10B TonA

text: ovary

text: Danio rerio

characteristic: organism

text: EcoRV

text: NotI

text: whole ovary, pooled from 25 animals

text: plasmid


text: National Cancer Institute / NIH

text: live

characteristic: INSDC status

text: Generic

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer 5'-GACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3', double-stranded cDNA was cloned into the NotI and EcoRV sites of pExpress-1 (EcoRV site destroyed upon ligation). Library was size-selected for an average insert size of 1.7 kb. Primary library, non-amplified. Use M13(-21) to generate 3' end reads and M13R to generate 5' end reads. Library was constructed by Open Biosystems (Huntsville, AL). Note: this is a (http://zgc.nci.nih.gov/) Zebrafish Gene Collection library.

text: DH10B TonA

text: whole body

text: Danio rerio

text: EcoRV

text: NotI

characteristic: title

text: plasmid


text: National Cancer Institute / NIH

text: live

text: Generic

characteristic: NCBI submission model

text: cDNA was primed using oligo-dT primer: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3' and cloned into the EcoRV/NotI sites of pExpress-1. Size-selection >1.3kb resulted in an average insert size of 1.4kb. This is a normalized primary library to Cot5 (non-normalized primary library is NIH_MGC_367) and was constructed by Express Genomics (Frederick, MD) for the (http://mgc.nci.nih.gov/) Mammalian Gene Collection

text: DH10B TonA

text: liver

text: Rattus norvegicus

text: EcoRV

text: NotI

text: whole liver, pool of 7

text: plasmid


text: National Cancer Institute / NIH

characteristic: INSDC center name

text: live

text: Generic

text: DH10B TonA

text: liver

text: Danio rerio

text: EcoRV

text: NotI

text: whole liver, pooled from 20 animals

text: plasmid


text: National Cancer Institute / NIH

text: live

text: Generic

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer 5'-GACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3', double-stranded cDNA was cloned into the NotI and EcoRV sites of pExpress-1 (EcoRV site destroyed upon ligation). Library was size-selected for an average insert size of 1.52 kb. Primary library, non-amplified. Use M13(-21) to generate 3' end reads and M13R to generate 5' end reads. Library was constructed by Open Biosystems (Huntsville, AL). Note: this is a (http://zgc.nci.nih.gov/) Zebrafish Gene Collection library.

text: DH10B TonA

characteristic: lab-host

text: whole body

text: Danio rerio

text: EcoRV

text: NotI

text: plasmid


text: National Cancer Institute / NIH

text: live

text: Generic

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer 5'-GACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3', double-stranded cDNA was cloned into the NotI and EcoRV sites of pExpress-1 (EcoRV site destroyed upon ligation). Library was size-selected for an average insert size of 1.5 kb. Primary library, non-amplified. Use M13(-21) to generate 3' end reads and M13R to generate 5' end reads. Library was constructed by Open Biosystems (Huntsville, AL). Note: this is a (http://zgc.nci.nih.gov/) Zebrafish Gene Collection library.

text: DH10B TonA

text: whole body

text: Danio rerio

text: EcoRV

text: NotI

text: plasmid


text: Sabanci University

text: live

text: Generic

text: Young, vigorously growing leaf, greenhouse-grown.

characteristic: dev-stage

text: Agrostis stolonifera

text: Leaf

text: Creeping bentgrass leaf tissue

text: Plasmid


text: Biotechnology Research Institute, Universiti Malaysia Sabah.

text: live

text: Generic

text: Sarawak (Smooth Cayenne)

characteristic: cultivar

text: etween green mature and ripe pineapples. Differentially espressed bands were isolated, cloned and then sequenced.

text: Mature stage

text: One Shot Mach1-T1 Chemically Competent E.coli

text: Fruit

text: Ananas comosus

text: Fruit flesh

text: Pineapple library

text: pCR 2.1-TOPO


text: University of Lausanne

text: live

text: Generic

text: Tags identified from Differential displays and cloned from cDNA directly synthesiszed from whole queen, male or worker RNA pools. Ants collected on the grounds of the University of Lausanne, Switzerland

characteristic: description

text: Adult

text: Lasius niger

text: male, female, and worker

characteristic: sex

text: Lausanne-UniL

characteristic: strain

text: Total

text: LN-DD


characteristic: External Id

text: The Breed & Biotechnology Key Laboratory of Sheep

text: live

text: Generic

text: mature proglottids

text: Moniezia benedeni

text: Shihezi

text: Moniezia benedeni Shihezi mature proglottids


characteristic: INSDC first public

text: live

text: Generic

text: gravid proglottids

text: Moniezia benedeni

text: Shihezi

text: Moniezia benedeni Shihezi gravid proglottids


text: USDA-ARS-NAA

text: live

text: Generic

text: Komata

text: cDNA clones from a forward subtraction library, soybean Komata challenged with Phakopsora pachyrhizi isolates.

text: Twenty-two day old soybeans (second trifoliate leaf stage)

text: Glycine max

text: Leaves

text: Forward JC.

text: pT7Blue vector

characteristic: vector


text: US Department of Agriculture (USDA), ARS, PSI

text: live

text: Generic

text: cDNA clones from healthy soybean roots, sequenced by Dupont.

text: Mature plants

text: Glycine max

text: Roots

characteristic: tissue-type

text: DupontLib.


text: Australian National University

characteristic: INSDC last update

text: live

text: Generic

text: ding to manufacturers specifications. Clones were recovered as inserts in pBluescript SK- and sequenced using the T3 primer.

text: postsettlement

text: Acropora millepora

text: EcoRI

text: XhoI

text: hermaphrodite

text: colony from Great Barrier Reef

text: Acropora millepora postsettlement library A

text: plasmid

text: pBluescript SK-


text: Australian National University

text: live

text: Generic

characteristic: NCBI submission package

text: prawn chip

text: Acropora millepora

text: EcoRI

text: XhoI

text: hermaphrodite

text: colony from Great Barrier Reef

text: Acropora millepora prawn chip library B

text: plasmid

text: pBluescript SK-


text: Australian National University

text: live

text: Generic

text: presettlement

text: Acropora millepora

characteristic: organism

text: EcoRI

text: XhoI

text: hermaphrodite

text: colony from Great Barrier Reef

text: Acropora millepora presettlement library C

text: plasmid

text: pBluescript SK-


text: Australian National University

text: live

characteristic: INSDC status

text: Generic

text: tography at 60 C in 0.12 M phosphate buffer (pH 6.8). After concentration, the eluate was used as template in PCR and products cloned into pGEM-T easy (Promega). Sequencing was carried out using the T3 primer.

text: presettlement

text: Acropora millepora

text: EcoRI

text: XhoI

text: hermaphrodite

text: colony from Great Barrier Reef

text: Acropora millepora presettlement library GS01

text: plasmid

text: pBluescript SK-


text: National University of Singapore

text: live

text: Generic

text: Sistrurus catenatus edwardsi

text: Venom gland

text: Snake venom gland SMART cDNA library

characteristic: title


text: University of Agricultural Sciences (UAS), GKVK, Bangalore

text: live

text: Generic

characteristic: NCBI submission model

text: Genomic DNA was isolated from 4 week-old healthy mulberry leaves,and PCR amplified using specific primers (Cer6F5'GCTTCAACCTCTCCGGCATGGGC3')Cer6R(5'GTCCAGGTTCTTCTGCAGCTCG3') the amlified product was cloned into T/A cloning vector pTZ57R/T

text: 4 week old leaves

text: Escherichia coli

text: Leaf

characteristic: organ

text: Morus alba

text: HindIII

text: Bisexual

text: Leaf tissue

text: Genomic DNA of mulberry


text: AgResearch Ltd.

characteristic: INSDC center name

text: live

text: Generic

text: Bovine mastitic mammary gland (mastitis affected cisternal) cDNA library

text: Bos taurus

text: Mastitic mammary gland (mastitis affected cisternal)

text: BMCA

text: plasmid

text: pBK-CMV


text: AgResearch Ltd

text: live

text: Generic

text: A normalised cDNA library was prepared by Clontech from RNA supplied by AgResearch. The cDNA clones were sequenced by TIGR

text: Trichosurus vulpecula

text: Approximately equal quantities of RNA from Kidney Cortex and Kidney Medulla were pooled for cDNA library generation

text: plasmid

text: PDNRLIB


text: AgResearch Ltd

text: live

text: Generic

text: Trichosurus vulpecula

text: RNA prepared from Liver was used for cDNA library generation

text: plasmid

text: PDNRLIB


text: Universita degli Studi dell'Insubria

text: live

text: Generic

text: Perca fluviatilis

text: adult brain and liver

text: P. fluviatilis liver minus brain cDNA subtraction library


text: The First Institute of Oceanography, SOA

text: live

text: Generic

text: E.coli DH5

characteristic: lab-host

text: Apostichopus japonicus

text: Express Library of The Apostichopus japonicus regenerating intestine

text: Plasmid

text: pBluescript


text: live

text: Generic

text: E.coli DH5

text: Apostichopus japonicus

text: The Library of The Apostichopus japonicus normal intestine

text: Plasmid

text: pBluescript


text: University of Georgia

text: live

text: Generic

characteristic: cultivar

text: Eleusine coracana subsp. coracana

text: Leaf

text: Finger millet pTriplEx2 library

text: Lambda TriplEx2


text: Department of Primary Industries

text: live

text: Generic

text: SMART PCR cDNA synthesis kit (Clontech, USA). pGEM-T Easy vector system I (Promega, USA). E.coli XL10- Gold ultracompetent cells (Stratagene, USA).

characteristic: description

text: Lolium perenne

text: subtractive library-12 weeks infected/uninfected ryegrass leaf sheaths


text: AgResearch Grasslands

text: live

text: Generic

text: perennial ryegrass NuiD

text: Lolium perenne

text: N. lolii Lp19

text: SSH Nle+M (SSH of Lolium perenne infected and uninfected with Neotyphodium lolii)


text: National Institute of Agricultural Biotechnology

text: live

text: Generic

text: Jemalong

text: UV-B irradiated 5min day-1 for 10 days. Subtracted cDNA was inserted into pGEM T-Easy vector.

text: 30 days after gernimation

characteristic: dev-stage

text: DH5 alpha

text: Medicago truncatula

text: EcoRI

text: EcoRI

text: leaf

text: Subtracted medicago cDNA library specific for UV-B irradiation

text: Plasmid

text: pGEM T-Easy vector


text: IFAPA-CIFA Churriana CICE-Junta de Andalucia.

text: live

text: Generic

text: Carisma

text: Unrippening Berries (Green fruit)

text: E. Coli

text: Fruit

text: Fragaria x ananassa

text: EcoRI

text: XhoI

text: Receptacle

text: Strawberry Receptacle Green Fruit Library

text: PBK-CMV


text: live

text: Generic

text: Carisma

text: Unrippening Berries (Green fruit)

text: E. Coli

text: Fruit

text: Fragaria x ananassa

text: EcoRI

text: XhoI

text: Achene

text: Strawberry Achene Green Fruit Library

text: PBK-CMV


text: live

text: Generic

text: Chandler

text: Red fruit

text: E. Coli

text: Fruit

text: Fragaria x ananassa

text: EcoRI

text: EcoRI

text: Achene and receptacle

text: Strawberry Red Fruit Library

text: pBS


text: live

text: Generic

text: Chandler

text: Red fruit - Green fruit

text: E. Coli

text: Fruit

text: Fragaria x ananassa

text: EcoRI

text: EcoRI

text: ACHENE AND RECEPTACLE

text: Strawberry Subtractive (Red - Green) Library

text: pBS


text: Nanjing Forestry University

text: live

text: Generic

text: leaves

text: Populus deltoides

text: differential display sequences


text: Sanger Institute

text: live

text: Generic

text: SalI_NotI cut cDNA was then ligated into pSPORT1 with SalI at the 5' end and NotI at the 3' end.

text: myoblast

text: E. coli

text: Danio rerio

text: SalI

text: NotI


text: Max-Planck-Institut fuer Molekulare Genetik

text: live

text: Generic

text: country: France:Villefranche

text: mesenchyme blastula and swimming blastula stage

text: Paracentrotus lividus

text: whole embryo

text: RZPD MPMGp1172 Paracentrotus lividus MBSB


text: live

text: Generic

text: country: France:Villefranche

text: unfertilised egg

text: Paracentrotus lividus

text: egg

text: RZPD MPMGp1174 Paracentrotus lividus EGG


text: Andres Bello University

text: live

text: Generic

text: O`Henry

text: DH10B E.coli

text: Fruit

text: Prunus persica

text: XhoI

text: SmaI

text: Mesocarp

text: Plasmid


text: College of Biological Sciences and Technology

text: live

text: Generic

text: DDRT-PCR, pCF-T vector

text: root

text: Populus euphratica

text: root cap

text: Populus euphratica hydrotropism differential display


text: The Institute for Genomic Research (TIGR; www.tigr.org); Fruit Laboratory, USDA (USDA); Plant Molecular and Cellular Biology Program and Horticultural Sciences Department University of Florida (U. of FA)

text: live

text: Generic

text: Hawaii 4

text: Fragaria vesca

text: cold-stressed Fragaria vesca seedlings cDNA library


text: Institute of Hydrobiology, the Chinese Academy of Sciences

text: live

text: Generic

text: Tetrahymena thermophila strain BF5 (provided by the Department of Biology, East of China Normal University, Shanghai, China). Cells cultured for 24 hours in medium containing 5 parts per billion tributylin (TBT).

text: Tetrahymena thermophila

characteristic: strain

text: Suppression Subtractive Hybridization Libraries of Tetrahymena thermophila Exposed in tributyltin (TBT)


characteristic: External Id

text: J. Craig Venter Institute (JCVI; www.jcvi.org)

text: live

text: Generic

text: starved cells, strain SB210, cells starved in 10mM Tris and harvested at 1, 2, 4.5 and 6 hrs.

text: Tetrahymena thermophila

text: Tetrahymena thermophila EST library strain SB210, starved


text: The Institute for Genomic Research (TIGR; www.tigr.org)

characteristic: INSDC first public

text: live

text: Generic

text: log-phase vegetatively growing cells, rich medium

text: Tetrahymena thermophila

text: Tetrahymena thermophila EST library strain SB210


text: live

text: Generic

text: conjugating cells, mating of strains CU428 and B2086, cells harvested at 3, 6, and 10 hours after mixing

text: Tetrahymena thermophila

text: CU428 and B2086 (conjugated)

text: Tetrahymena thermophila EST library, conjugated strains CU428 and B2086


text: University of Georgia

text: live

text: Generic

text: Eleusine coracana subsp. africana

text: leaf

text: Finger millet pTriplEx2 library 2

text: Lambda TriplEx2

characteristic: vector


text: West China Hospital, Sichuan University

text: live

text: Generic

text: adult

text: Sus scrofa

text: male

characteristic: sex

text: liver

characteristic: tissue-type

text: Banna Minipig Inbred line liver tissue cDNA library

text: Phage

text: ZAP Express Vector


text: Universite du Quebec a Montreal

characteristic: INSDC last update

text: live

text: Generic

characteristic: NCBI submission package

text: Norstar

text: The cDNA was cloned using Eco RI/ Not I adaptors.

text: Cold treated for 24 hours

text: Triticum aestivum

text: Crown and leaf

text: Cold treated wheat cDNA library

text: Lambda ZAP II


text: BC Cancer Agency

text: live

characteristic: INSDC status

text: Generic

text: 4 years

text: ElectroMAX DH10B T1 Phage-Resistant Cells

text: Bos taurus

characteristic: organism

text: SalI (5' end of cDNA)

text: NotI (3' end of cDNA)

text: female

text: Hereford

text: Mammary Gland

text: pCMV SPORT 6.0


text: University of Alberta

text: live

text: Generic

characteristic: NCBI submission model

text: 7 years

text: Testis

text: Bos taurus

text: SalI (5' end of cDNA)

text: NotI (3' end of cDNA)

text: male

text: Hereford

text: Testis

characteristic: title

text: pCMV SPORT 6.0


text: BC Cancer Agency

characteristic: INSDC center name

text: live

text: Generic

text: 8.5 months

text: Thymus

characteristic: organ

text: Bos taurus

text: SalI (5' end of cDNA)

text: NotI (3' end of cDNA)

text: female (calf)

text: Hereford

text: Thymus

text: pCMV SPORT 6.0


text: University of Alberta

text: live

text: Generic

text: 6 months

characteristic: lab-host

text: Fetal Pons

text: Bos taurus

text: SalI (5' end of cDNA)

text: NotI (3' end of cDNA)

text: male

text: Hereford

text: Fetal Pons

text: pCMV SPORT 6.0


text: University of Alberta

text: live

text: Generic

text: 6 months

text: Fetal Lung

text: Bos taurus

text: SalI (5' end of cDNA)

text: NotI (3' end of cDNA)

text: male

text: Hereford

text: Fetal Lung

text: pCMV SPORT 6.0


text: University of Liverpool/School of Biological Sciences

text: live

text: Generic

text: DH5a E. coli

text: Oncorhynchus mykiss

text: liver

text: Rainbow trout liver SSH library from warm, cold and hypoxic stress (rtll)

text: pGEM-Teasy vector


text: live

text: Generic

text: DH5a E. coli

text: Oncorhynchus mykiss

text: muscle

text: Rainbow trout muscle SSH library from warm, cold and hypoxic stress (rtlm)

text: pGEM-Teasy vector


text: live

text: Generic

text: DH5a E. coli

text: Oncorhynchus mykiss

text: brain

text: Rainbow trout brain SSH library from warm, cold and hypoxic stress (rtlb)

text: pGEM-Teasy


text: University of Helsinki

text: live

text: Generic

text: country: Finland:Haapastensyrjae, Loppi

characteristic: description

text: developing and mature

text: n=30 years

characteristic: isolate

text: Picea abies

text: xylem


text: University of Helsinki

text: live

text: Generic

text: country: Finland:Haapastensyrjae, Loppi

text: cell elongation and expansion

characteristic: dev-stage

text: n=21 years

text: Picea abies

text: xylem fraction


characteristic: External Id

text: University of Helsinki

text: live

text: Generic

text: country: Finland:Haapastensyrjae, Loppi

text: secondary cell wall synthesis and lignification

text: n=21 years

text: Picea abies

text: xylem fraction


text: University of Helsinki

characteristic: INSDC first public

text: live

text: Generic

text: country: Finland:Haapastensyrjae, Loppi

text: mature

text: n=21 years

text: Picea abies

text: xylem fraction


text: University of Helsinki

text: live

text: Generic

characteristic: cell-line

text: country: Finland:Haapastensyrjae, Loppi

text: mostly undifferentiated cells, some differentiated to tracheids

text: n=1 day

text: Picea abies

text: lignin-forming tissue culture


text: Max-Planck-Institute for Terrestrial Microbiology

text: live

text: Generic

text: DAOM 181602

text: Daucus carota

text: Rhizophagus intraradices

text: extraradical mycelium

text: Glomus intraradices DAOM 181602 extraradical mycelium


text: Sichuan Agriculture University

text: live

text: Generic

text: Sequence was from the R. solani-inducible differentially expressed genes in maize (Zea mays L.) using suppression subtractive hybridization

text: Zea mays

text: Expressed sequence tags from the Forward SSH library

characteristic: vector


text: Sichuan Agriculture University

text: live

text: Generic

text: Zea mays

text: Expressed sequence tags from the Reverse SSH library


text: University of Washington

text: live

text: Generic

text: young adult

text: DH10B E. coli bacterial cells, T1 phage resistant

text: Macaca nemestrina

text: Not I

text: Eco RV

text: male

characteristic: sex

text: testis

characteristic: tissue-type

text: Pigtailed macaque testis library

text: plasmid


text: University of Washington

characteristic: INSDC last update

text: live

text: Generic

characteristic: NCBI submission package

text: young adult

text: Macaca nemestrina

text: Not I

text: Eco RV

text: female

text: ovary

text: Pigtailed macaque ovary library

text: plasmid


text: Universite du Quebec a Montreal

text: live

characteristic: INSDC status

text: Generic

text: Norstar

characteristic: cultivar

text: cDNAs were amplified with a gene specific primer and a universal primer (M13F or M13R) using libraries from the Functional Genomics of Abiotic Stress in Wheat project. PCR products were cloned in pCR4-TOPO for sequencing.

text: Triticum aestivum

characteristic: organism

text: Crown, leaf and root

text: Genes amplified from wheat FGAS cDNA libraries


text: Universite du Quebec a Montreal

characteristic: INSDC center name

text: live

text: Generic

characteristic: NCBI submission model

text: Norstar

text: cDNAs were directionally cloned with Sal I adaptor (GTCGACCCACGCGTCCG) on the 5' end and Not I primer-adaptor (GCGGCCGCCC(T15)) on the 3' end.

text: Triticum aestivum

text: Crown, leaf and root

text: Stress treated wheat cDNA libraries from the FGAS project

characteristic: title


text: UMR INRA-ENSAR Genetique Animale

text: live

text: Generic

text: Clone distribution : AGENAE Resource centre. Francois PIUMI, Francois.Piumi@jouy.inra.fr, INRA, CEA Radiobiologie et Etude du genome (LREG), Domaine de Vilvert, 78352, Jouy-en-Josas cedex, FRANCE, +33 (0) 1.34.65.28.02, +33 (0) 1.34.65.22.73

characteristic: description

text: adult (9 weeks)

text: Gallus gallus

text: liver

text: Gallus gallus DDChick2_Met. library (gcan)


text: Institute of Veterinary Medicine

text: live

text: Generic

text: breed: Holstein ; microvesicular associated RNA

text: Bos taurus

text: blood serum

text: Bos taurus blood serum


text: University of Helsinki

text: live

text: Generic

text: country: Finland:Haapastensyrjae, Loppi

text: n=3 days

characteristic: isolate

text: Picea abies

text: lignin-forming tissue culture


text: University of Helsinki

text: live

text: Generic

text: country: Finland:Haapastensyrjae, Loppi

characteristic: dev-stage

text: n=2 days

text: Picea abies

text: lignin-forming tissue culture


characteristic: External Id

text: Centre Hospitalier Universitaire Vaudois

text: live

text: Generic

text: Trichophyton rubrum

characteristic: strain

text: Trichophyton rubrum CHUV862.00


text: Genoscope - Centre National de Sequencage

characteristic: INSDC first public

text: live

text: Generic

text: ecotype: Sierra de Guadarrama. Spain. The library was made from buds of adult trees. A mixture of genotypes were used. Oligo-dT primed cDNA was directionally cloned into the EcoRI-XhoI lambda-ZAP vector arms and mass-excised to form a pBluescript phagemid.

text: adult trees

text: Pinus pinaster

text: developing buds collected on several trees

text: Uni-ZAP XR


text: Seoul National University

text: live

text: Generic

text: Sinpaldalkong 2

text: mRNA from 2 weeks-old leaves harvested 1 week after Bradyrhizobium japonicum inoculation

text: E. coli JM109

characteristic: lab-host

text: Glycine max

text: Leaf

text: Transcript-derived fragments specifically found in Sinpaldalkong 2 (wild type)

text: plasmid

text: pGEM

characteristic: vector


text: Seoul National University

text: live

text: Generic

text: E. coli JM109

text: Glycine max

text: Leaf

characteristic: tissue-type

text: Transcript-derived fragments specifically found in SS2-2 (supernodulating mutant)

text: plasmid

text: pGEM


text: Institute of Botany, Chinese Academy of Sciences

characteristic: INSDC last update

text: live

text: Generic

text: Jisheng No.1

text: Seeds of Leymus chinensis were germinated at 4o under dark conditions and seedlings were grown under experimental field. Leaf of two-month-old plants were harvested and stored at -70o for RNA extraction and cDNA construction. The RNA was extracted with TRIZOL Reagent (GIBCO) and cDNA library was constructed with Creator TM SMART TM cDNA Library Construction Kit (Cat No. K1053-1; BD Biosciences-Clontech). The cDNA was ligated to the Sfi I-digested, dephosphorylated pDNR-LIB vector.

text: Two-month-old plants

text: E.coli

text: Leymus chinensis

text: Sfi I

text: Sfi I

text: leaf

text: Leymus chinensis cv. Jisheng No.1 leaf cDNA library

text: Plasmid

text: pDNR-LIB


text: Universidade Federal de Sao Paulo - Escola Paulista de Medicina

text: live

text: Generic

characteristic: NCBI submission package

text: The poly A+ RNA was isolated by chromatography on oligo(dT) cellulose column. The cDNA synthesis was carried out using the SuperScript Lambda System for cDNA Synthesis and l Cloning. The cDNA molecules higher than 500 bp were inserted into lambda ZipLox Not I/Sal I Arms vector and packaged using Gigapack III Gold Packaging Extract.

text: amastigote

text: Escherichia coli DH10B

text: Leishmania amazonensis

text: Sal I

text: Not I

text: LEM690 (MHOM/BR/1973/M2269)

text: Leishmania (Leishmania) amazonensis amastigote cDNA library

text: Phagemid

text: Lambda ZipLox Not I / Sal I Arms Vector


text: University of California at Irvine

text: live

characteristic: INSDC status

text: Generic

text: crawler and pre-pupae

text: wing disc

characteristic: organ

text: Bicyclus anynana

text: Bicyclus anynana wings - CPP


text: University of California at Irvine

text: live

text: Generic

text: pupae 24 hrs after pupation

text: wing disc

text: Bicyclus anynana

characteristic: organism

text: Bicyclus anynana wings - 24H pupae


text: University of California at Irvine

characteristic: INSDC center name

text: live

text: Generic

text: pupae 48 hrs after pupation

text: wing disc

text: Bicyclus anynana

text: Bicyclus anynana wings - 48H pupae


text: University of California at Irvine

text: live

text: Generic

characteristic: NCBI submission model

text: pupae 72 hrs after pupation

text: wing disc

text: Bicyclus anynana

text: Bicyclus anynana wings - 72H pupae


text: University of California at Irvine

text: live

text: Generic

text: fifth-instar larvae

text: wing disc

text: Bicyclus anynana

text: Bicyclus anynana wings - 5TH instar larvae

characteristic: title


text: Universite Montpellier2

text: live

text: Generic

text: Spodoptera frugiperda

text: Fat Body

text: Spodoptera frugiperda Fat Body cDNA Library

text: Plasmid

text: pTriplex2 (Clontech)


text: Universite Montpellier2

text: live

text: Generic

text: Spodoptera frugiperda

text: Hemocytes

text: Spodoptera frugiperda Hemocytes cDNA Library

text: Plasmid

text: pTriplex2 (Clontech)


text: Universite Montpellier2

text: live

text: Generic

text: Spodoptera frugiperda

text: Midgut

text: Spodoptera frugiperda Midgut cDNA Library

text: Plasmid

text: pTriplex2 (Clontech)


text: University of Southern Mississippi

text: live

text: Generic

characteristic: cell-line

text: Spodoptera frugiperda


text: Universite des Sciences et Technologies de Lille

text: live

text: Generic

text: Witloof

characteristic: cultivar

text: Cichorium intybus

text: cDNA library from chicory suspension cultures

text: plasmid


text: Norwegian University of Sience and Technology

text: live

text: Generic

text: Clones obtained by subtractive hybridizaion of liver samples of juvenile Atlantic salmon exposed to a complex mixture of xenobiotica

characteristic: description

text: Juvenile

characteristic: dev-stage

text: Liver

text: Salmo salar

text: SalArray SSH cDNA library

text: pGEM T-easy cloning vector


characteristic: External Id

text: University of California at Davis (UCD)

text: live

text: Generic

text: The standard library was constructed from multiple sources of RNA from a single genotype. cDNAs were then size-fractionated, directionally cloned into a custom medium-copy vector and transformations made with three size classes (X-large,Y-medium and Z-small) to minimize size bias due to differential transformation efficiency. Details of each source of RNA and library construction can be obtained at http://cgpdb.ucdavis.edu/

text: E.coli

text: Helianthus annuus

characteristic: strain

text: CHA(XYZ) common wild sunflower

text: Plasmid

text: pBRcDNASfiAB


text: University of California at Davis (UCD)

characteristic: INSDC first public

text: live

text: Generic

text: E.coli

characteristic: lab-host

text: Helianthus petiolaris

text: CHP(XYZ) plains sunflower

text: Plasmid

text: pBRcDNASfiAB


text: University of California at Davis (UCD)

text: live

text: Generic

text: Salinas

text: The normalized library was constructed from multiple sources of RNA from a single genotype using Trimmer-Direct kit. cDNAs were then size-fractionated, directionally cloned into a custom medium-copy vector and transformations made with three size classes (L-large, M-medium and S-small) to minimize size bias due to differential transformation efficiency. Details of each source of RNA and library construction can be obtained at http://cgpdb.ucdavis.edu/

text: E.coli

text: Lactuca sativa

text: CLS(LMS) lettuce sativa

text: Plasmid

text: pBRcDNASfiAB

characteristic: vector


text: University of California at Davis (UCD)

characteristic: INSDC last update

text: live

text: Generic

text: E.coli

text: Taraxacum officinale

text: CTO(XYZ) dandelion

text: Plasmid

text: pBRcDNASfiAB


text: Maize Research Institute in Sichuan Agriculture University of China

text: live

text: Generic

characteristic: NCBI submission package

text: Zea mays

text: Maize Embryonic Callus Regeneration SSH cDNA Library

text: Plasmid

text: pGEM-T