text: live

text: Generic

text: E. coli Top 10

text: Sparus aurata

text: head kidney

text: Aquafirst Sea Bream SSH selection confinement head kidney early (gsgy01)

text: plasmid

text: PCR 2.1


status: PUBLIC

characteristic: External Id

text: National University of Ireland Galway (NUIG)

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: E. coli Top 10

tag: attribute

characteristic: lab-host

text: Sparus aurata

characteristic: organism

text: liver

characteristic: tissue-type

text: Aquafirst Sea Bream SSH selection confinement liver early (gsgl02)

characteristic: title

text: plasmid

text: PCR 2.1

characteristic: vector


text: INRA-SCRIBE

text: live

text: Generic

text: E. coli Top 10

text: Sparus aurata

text: intestine

text: Aquafirst Sea Bream SSH selection pathogen intestine (gsgi08)

text: plasmid

text: PCR 2.1


text: live

text: Generic

text: E. coli Top 10

text: Sparus aurata

text: liver

text: Aquafirst Sea Bream SSH selection confinement liver late (gsgl03)

text: plasmid

text: PCR 2.1


text: live

text: Generic

text: E. coli Top 10

text: Sparus aurata

text: head kidney

text: Aquafirst Sea Bream SSH selection pathogen head kidney (gsgy07)

text: plasmid

text: PCR 2.1


text: live

text: Generic

text: E. coli Top 10

text: Sparus aurata

text: intestine

text: Aquafirst Sea Bream SSH selection pathogen intestine (gsgi07)

text: plasmid

text: PCR 2.1


text: Clemson University

text: live

text: Generic

text: BX3, clone Vanuxum

characteristic: cultivar

text: CMFME A Leaf fraction 678. Collected by Fred Hebard on 6/5/2007. Flowers collected, ground in RNAlater and shipped in the same. Tissue sent to Bill Powell. Size subtracted. Sequencing performed by David Henry at Clemson University Genomics Institute.

characteristic: description

text: mature

characteristic: dev-stage

text: Castanea mollissima

text: flowers

text: CMFMEa

text: pDNR-lib


text: Clemson University

text: live

text: Generic

text: BX3, clone Vanuxum

text: CMFME B Leaf fraction 9. Collected by Fred Hebard on 6/5/2007. Flowers collected, ground in RNAlater and shipped in the same. Tissue sent to Bill Powell. Size subtracted. Sequencing performed by David Henry at Clemson University Genomics Institute.

text: mature

text: Castanea mollissima

text: flowers

text: CMFMEb

text: pDNR-lib


text: Clemson University

text: live

text: Generic

text: BX3, clone Vanuxum

text: CMFME C Leaf fraction 10. Collected by Fred Hebard on 6/5/2007. Flowers collected, ground in RNAlater and shipped in the same. Tissue sent to Bill Powell. Size subtracted. Sequencing performed by David Henry at Clemson University Genomics Institute.

text: mature

text: Castanea mollissima

text: flowers

text: CMFMEc

text: pDNR-lib


text: Clemson University

text: live

text: Generic

text: Greg Miller seedling ortets

text: CMLME A Leaf fraction 567. Created by pooling of mature leaves from 3 seedlings. Collected by Bill Powell in in summer 2007. Material collected from Chinese chestnut seedlings from seeds obtained from Greg Miller, Empire Chestnut Company, 3276 Empire Road SW, Carrolton, OH 44615, phone #330-627-3181. Size subtracted, fraction 567. Sequencing performed by David Henry at Clemson University Genomics Institute.

text: mature

text: Castanea mollissima

text: leaves

text: CMLMEa

text: pDNR-lib


text: Clemson University

text: live

text: Generic

text: Greg Miller seedling ortets

text: CMLME B Leaf fraction 8. Created by pooling of mature leaves from 3 seedlings. Collected by Bill Powell in in summer 2007. Material collected from Chinese chestnut seedlings from seeds obtained from Greg Miller, Empire Chestnut Company, 3276 Empire Road SW, Carrolton, OH 44615, phone #330-627-3181. Size subtracted, fraction 8. Sequencing performed by David Henry at the Clemson University Genomics Institute.

text: mature

text: Castanea mollissima

text: leaves

text: CMLMEb

text: pDNR-lib


text: Clemson University

text: live

text: Generic

text: Greg Miller seedling ortets

text: CMRME A Root fraction 567. Created by pooling of roots from 2 seedlings. Collected by Bill Powell in in summer 2007. Material collected from Chinese chestnut seedlings from seeds obtained from Greg Miller, Empire Chestnut Company, 3276 Empire Road SW, Carrolton, OH 44615, phone #330-627-3181. Size subtracted, fraction 567. Sequencing performed by David Henry at the Clemson University Genomics Institute.

text: mature

text: Castanea mollissima

text: roots

text: CMRMEa

text: pDNR-lib


text: Clemson University

text: live

text: Generic

text: Greg Miller seedling ortets

text: CMRME B Root fraction 8. Created by pooling of roots from 2 seedlings. Collected by Bill Powell in in summer 2007. Material collected from Chinese chestnut seedlings from seeds obtained from Greg Miller, Empire Chestnut Company, 3276 Empire Road SW, Carrolton, OH 44615, phone #330-627-3181. Size subtracted, fraction 8. Sequencing performed by David Henry at the Clemson University Genomics Institute.

text: mature

text: Castanea mollissima

text: roots

text: CMRMEb

text: pDNR-lib


text: Clemson University

text: live

text: Generic

text: CMSME A Stem fraction 678. Created by pooling of bark (secondary phoem, cortex, and primary periderm) not wounded, wounded, and wounded plus inoculated in wound from tree GR119. Collected by Fred Hebard. Inoculated 6/25/06, collected 6/30/06 and 7/12/06. Tissue sent to Scott DiLoreto. Size subtracted, fraction 678. Sequencing performed by David Henry at the Clemson University Genomics Institute.

text: mature

text: Castanea mollissima

text: Bark (secondary phoem, cortex, and primary periderm) not wounded, wounded, and wounded plus inoculated in wound

text: CMSMEa

text: pDNR-lib


text: Clemson University

text: live

text: Generic

text: CMSME B Stem fraction 9. Created by pooling of bark (secondary phoem, cortex, and primary periderm) not wounded, wounded, and wounded plus inoculated in wound from tree GR119. Collected by Fred Hebard. Inoculated 6/25/06, collected 6/30/06 and 7/12/06. Tissue sent to Scott DiLoreto. Size subtracted, fraction 9. Sequencing performed by David Henry at the Clemson University Genomics Institute.

text: mature

text: Castanea mollissima

text: CMSMEb

text: pDNR-lib


text: Pastoral Genomics, c/o ViaLactia Biosciences (NZ) Ltd

text: live

text: Generic

text: Bronsyn LE

text: Etiolated seedlings

text: Lolium perenne

text: Diecious

characteristic: sex

text: All tissues

text: Perennial ryegrass etiolated seedlings library (LPE)

text: pBluescriptIISK+


text: live

text: Generic

text: Bronsyn LE

text: Three week seedlings (heat-stressed for 8 hrs at 37 deg.)

text: Lolium perenne

text: Diecious

text: Leaf and stem

text: Perennial ryegrass 3 wk seedlings, heat-stressed for 8 hrs at 37 deg. library (LPH1)

text: pBluescriptIISK+


text: live

text: Generic

text: Bronsyn LE

text: Emerging spike (10 days)

text: Lolium perenne

text: Diecious

text: Flower spike

text: Perennial ryegrass flower spike emerging, 10 days library (LPF2)

text: pBluescriptIISK+


text: live

text: Generic

text: Bronsyn LE

text: Three week seedlings (cold-acclim. for 7 days at 4 deg.)

text: Lolium perenne

text: Diecious

text: Leaf and stem

text: Perennial ryegrass 3 wk seedlings, cold-acclim. for 7 days at 4 deg. library (LPC1)

text: pBluescriptIISK+


text: live

text: Generic

text: Bronsyn LE

text: Prior to stalk yellowing (7 weeks)

text: Lolium perenne

text: Diecious

text: Seed

text: Perennial ryegrass seed prior to stalk yellowing, 7 weeks library (LPF4)

text: pBluescriptIISK+


text: live

text: Generic

text: Bronsyn LE

text: Lolium perenne

text: Diecious

text: Root

text: Perennial ryegrass 3 wk seedlings, heat-stressed for 8 hrs at 37 deg. library (LPH2)

text: pBluescriptIISK+


text: live

text: Generic

text: Bronsyn LE

text: Flag stage (unemerged, 0 days)

text: Lolium perenne

text: Diecious

text: Flower spike

text: Perennial ryegrass flower spike unemerged, 0 days library (LPF1)

text: pBluescriptIISK+


text: live

text: Generic

text: Bronsyn LE

text: Lolium perenne

text: Diecious

text: Root

text: Perennial ryegrass 3 wk seedlings, cold-acclim. for 7 days at 4 deg. library (LPC2)

text: pBluescriptIISK+


text: College of Horticulture, Northwest A&F University

text: live

text: Generic

text: Dangshansu

text: Total RNA was extracted from leaves harvested 0.5, 1, 2, 3, 5, 7 and 9 days after inoculation with Venturia nashicola. The PolyATtract mRNA Isolation System III (Promega) was employed to isolate mRNA. Substractive Hybridization was conducted with the PCR-Select cDNA Subtraction Kit(Clontech). The SSH-cDNA fragments were sub-cloned into the pGEM-T easy vector (Promega) and transformed into E.coli Top10 cells.

text: 0.5, 1, 2, 3, 5, 7 and 9 days after inoculated with Venturia nashicola

text: E.coli Top10

text: Pyrus x bretschneideri

text: leaves

text: Supression Substractive Hybridization cDNA library of pear inoculated with Venturia nashicola

text: pGEM-T easy


text: Biomanufacturing Research Institute & Technology Enterprise, North Carolina Central University

text: live

text: Generic

text: Golden Pothos

text: The RNAs for making first strain cDNAs were isolated from tissue culture regenerated pale yellow and green plants.

text: Epipremnum aureum

text: leaves

text: Golden Pothos pale yellow and green tissues of cDNA suppression subtractive hybridization library


text: Max-Planck-Institute for Heart and Lung Research

text: live

text: Generic

text: cDNA from regenerating newt heart tissue, 14 days after ventricular damage

text: adult

text: heart

characteristic: organ

text: Notophthalmus viridescens

text: regenerating heart tissue

text: non normalized cDNA library of 14d regenerating newt heart

text: pDNR-LIB


characteristic: External Id

text: Uppsala University

text: live

text: Generic

text: E. coli Top 10

text: Sparus aurata

text: gill

text: confinement gill early(fw+rv) (gsug01)

text: plasmid

text: PCR 2.1


text: Institut Biologia Evolutiva (UPF-CSIC)

characteristic: INSDC center name

text: live

text: Generic

text: Blattella germanica

text: postvitellogenic ovary

text: Blattella germanica postvitellogenic ovary


text: Zhejiang Forestry University, Dong Hu Campus

characteristic: INSDC first public

text: live

text: Generic

text: Vegetative growth

text: Stem

text: Carya cathayensis

text: Cambium

text: cDNA-AFLP in hickory


text: McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University,

characteristic: INSDC last update

text: live

text: Generic

text: cDNA was synthesized from total RNA isolated and fragments amplified by PCR

text: Adult

text: Malpighian tubule

text: Anopheles gambiae

text: Female

text: Keele

characteristic: strain

text: Malpighian tubule

text: Anopheles gambiae Adult female malpighian tubule


text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Adult

text: Salivary gland

text: Anopheles gambiae

text: Female

text: Keele

text: Salivary gland

text: Anopheles gambiae Adult female salivary gland


text: live

text: Generic

characteristic: NCBI submission package

text: Adult

text: Ovary

text: Anopheles gambiae

text: Female

text: Keele

text: Ovary

text: Anopheles gambiae Adult female Ovary


text: Mount Desert Island Biological Laboratory

text: live

text: Generic

text: ZFL

characteristic: cell-line

text: Livers (5-10mg) were dissected from 2-3 adult Zebrafish under sterile conditions, pooled into 3 mls of 0.02% trypsin, 1 mM EDTA in PBS and incubated for 5 minutes at room temperature. Tissues were gently dissociated by pipeting to obtain small cell aggregates and single cells. Cells were pelleted and washed once in LDF basal nutrient medium (50% Dulbeco's modified Eagle's, 35% Leibovitz's L-15 and 15% Ham's F-12 with sodium bicarbonate (0.15 mg/ml), HEPES buffer (15mM, pH 7.2, penicillin (200 U/ml), streptomycin sulfate (200 ug/ml)and ampicillin (25 ug/ml)and finally resuspended in 1 ml of LDF and incubated for 30 minutes at room temperature before an additional ml of LDF was added with the following supplements: 5% fetal bovine serum, 10 ug/ml insulin, 0.5% trout serum, 20 ng/ml EGF and 40 ug/ml trout embryo extract. Trout serum and embryo extract were prepared as previously described (Collodi, P; Kamei, Y; Ernst, T; Miranda, C; Buhler, D.R. and Barnes, D.W., 1992, Cell Biol. Toxicol. 8, 43-61; Collodi, P. and Barnes, D.W., 1990, Proc. Natl. Acad. Sci. USA 87, 3498-3502). Dishes were wrapped in Parafilm and incubated at 26oC. Culture medium was replaced once a week. After 2-3 weeks monolayer regions consisting of exclusively epithelial cell types were removed using cloning rings and expanded until a minimum of 20 population doublings had been achieved. The growth medium has since then been simplified to LDF containing 5% heat-inactivated FBS, 10 ug/ml insulin and 50 ng/ml EGF. The cells are available through the American Type Culture Collection (ATCC), cat# CRL-2643.

text: adult

text: DH10B (T1 and T5 phage resistance)

characteristic: lab-host

text: Liver

text: Danio rerio

characteristic: organism

text: EcoRI

text: NotI

text: Wild type

text: DRDBCa


text: Center for Genetic Engineering and Biotechnology

text: live

text: Generic

text: Nicotiana tabacum

text: root and stem

characteristic: tissue-type

text: tobacco root and stem SSH cDNA Library

characteristic: title


text: National University of Ireland, Galway

text: live

text: Generic

text: Suppression subtractive hybridization library of LPS- and TNFalpha-stimulated trout head kidney-isolated leucocytes

characteristic: description

text: Adult

text: Oncorhynchus mykiss

text: Leukocyte

text: Head Kidney stimulated leucocyte


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: normal greenhouse care. The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected >2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed and sequencing performed at the DOE Joint Genome Institute (Walnut Creek, CA).

text: Erythranthe guttata

text: SalI

text: NotI

text: hermaphrodite

text: roots, seedlings, maturing fruits/ovaries, callus

text: CBPF Mimulus guttatus IM62 roots, seedlings, fruits, callus (H)

characteristic: vector


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: normal greenhouse care. The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 0.6-2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed and sequencing performed at the DOE Joint Genome Institute (Walnut Creek, CA).

text: Erythranthe guttata

text: SalI

text: NotI

text: hermaphrodite

characteristic: sex

text: CBPG Mimulus guttatus IM62 roots, seedlings, fruits, callus (L)


text: DOE Joint Genome Institute

text: live

text: Generic

text: unopened

characteristic: dev-stage

text: Erythranthe lewisii

text: SalI

text: NotI

text: hermaphrodite

text: LF10 + SL9

text: floral buds

text: CBPH Mimulus lewisii floral buds (H)


characteristic: External Id

text: DOE Joint Genome Institute

text: live

text: Generic

text: unopened

text: Erythranthe lewisii

text: SalI

text: NotI

text: hermaphrodite

text: LF10 + SL9

text: floral buds

text: CBPI Mimulus lewisii floral buds (L)


text: DOE Joint Genome Institute

characteristic: INSDC center name

text: live

text: Generic

text: In Vivo Conditions: standard greenhouse, long days. The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected >2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed and sequencing performed at the DOE Joint Genome Institute (Walnut Creek, CA).

text: adult

text: Erythranthe guttata

text: SalI

text: NotI

text: hermaphrodite

text: floral buds

text: CCBC Mimulus guttatus IM62 floral buds (H)


text: DOE Joint Genome Institute

characteristic: INSDC first public

text: live

text: Generic

text: In Vivo Conditions: standard greenhouse, long days. The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 0.6-2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed and sequencing performed at the DOE Joint Genome Institute (Walnut Creek, CA).

text: adult

text: Erythranthe guttata

text: SalI

text: NotI

text: hermaphrodite

text: floral buds

text: CCBF Mimulus guttatus IM62 floral buds (L)


text: DOE Joint Genome Institute

characteristic: INSDC last update

text: live

text: Generic

text: In Vivo Conditions: standard greenhouse, long days. The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected 0.6-2kb and >2kb using 1.1% agarose gel electrophoresis then pooled and ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed and sequencing performed at the DOE Joint Genome Institute (Walnut Creek, CA).

text: adult

text: Erythranthe guttata

text: SalI

text: NotI

text: hermaphrodite

text: floral buds

text: CCBG Mimulus guttatus IM62 floral buds (H+L)


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: standard greenhouse, long days. The library was made from oligo(dT)-primed cDNA and cloned into vector pDNR-LIB (Clontech Laboratories, Inc., Mountain View, CA) using the SMART system (BD Biosciences, San Jose, CA). To generate the first strand, polyA RNA was primed with an oligo(dT) primer (5'-ATTCTAGAGGCCGAGGCGGCCGACATG(T)30 VN-3', where V = A, G, or C). The second strand was synthesized by PCR using primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' followed by digestion of the SfiI sites. Library is normalized. Library was created and sequencing performed by the DOE Joint Genome Institute (Walnut Creek, CA).

text: adult

text: Erythranthe guttata

text: SfiIA

text: SfiIB

text: hermaphrodite

characteristic: strain

text: floral buds

text: CCBX Mimulus guttatus IM62 floral buds (N)

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

characteristic: INSDC status

text: Generic

text: adult

text: Erythranthe guttata

text: SalI

text: NotI

text: roots

text: CCIC Mimulus guttatus IM62 roots (H)


text: DOE Joint Genome Institute

text: live

text: Generic

characteristic: NCBI submission model

text: adult

text: Erythranthe guttata

text: SalI

text: NotI

text: leaves

text: CCIF Mimulus guttatus IM62 leaves (H)


text: DOE Joint Genome Institute

text: live

text: Generic

characteristic: NCBI submission package

text: adult

text: Erythranthe guttata

text: SalI

text: NotI

text: floral buds

text: CCIG Mimulus guttatus DUN10 floral buds (H)


text: DOE Joint Genome Institute

text: live

text: Generic

text: adult

text: Erythranthe nasuta

text: SalI

text: NotI

text: SF

text: floral buds

text: CCIH Mimulus nasutus SF floral buds (L)


text: DOE Joint Genome Institute

text: live

text: Generic

text: adult

text: Erythranthe nasuta

text: SalI

text: NotI

text: SF

text: floral buds

text: CCII Mimulus nasutus SF floral buds (H)


text: Universite Laval

text: live

text: Generic

text: Library synthesis was performed by Evrogen using methodology described in Zhulidov et al. (2004) Nucleic Acids Res. 32, e37. Starting material is 0,3 ug of total RNA.

text: Pool of trees sampled at three different daylight times (early, mid-day, late) at three different seasonal points (june, july, august)

text: Stem from 35 year old trees

characteristic: organ

text: Picea glauca

text: Hermaphrodite (Monoecious)

text: Two known open-pollinated families (EPB 2348 and EPB 2349), 35 year old genetic trial, Lotbiniere, Quebec

text: Cambium and phloem region from normal vertical trees

text: GQ028 - Cambium / phloem scrappings (Normalized library )

text: Plasmid


text: Universite Laval

text: live

text: Generic

text: Library synthesis was performed by Evrogen using methodology described in Zhu et al. (2001) Biotechniques 30: 892-897. Starting material is 0,3 ug of total RNA

text: Pool of trees sampled at three different times (early, mid-day, late) at three different seasonal points (june, july, august)

characteristic: lab-host

text: Stem from 35 year old trees

text: Picea glauca

text: Hermaphrodite (Monoecious)

text: Partly-lignified differentiating secondary xylem from normal vertical trees

text: GQ030 - Xylem planings - daytime and seasonal

text: Plasmid


text: Indian Institute of Advanced Research

text: live

text: Generic

text: Total RNA was isolated from seed kernel of J.curcas L.. A PCR based library was constructed. The amplified product was fractionated through a cDNA size fractionation column (Invitrogen). The fractions were cloned one at a time in pDrive/pJET vector and transformed in E.coli. The transformants were screened using restriction endonuclease. The positive transformants were sequenced from both ends. The sequences obtained using SP6 promoter are reverse complemented. This research work has been undertaken as a part of a project sanctioned by Department of Biotechnology, New Delhi, India.

text: Jatropha curcas

characteristic: organism

text: EcoRI

text: EcoRI

text: Seed kernel

text: Jatropha curcas L. seed cDNA library

text: Plasmid

text: pDrive


text: Stony Brook University

text: live

text: Generic

text: adults

text: Mercenaria mercenaria

text: hemocytes

characteristic: tissue-type

text: cDNA libraries from clams hemocytes


text: National University of Ireland, Galway

text: live

text: Generic

text: Suppression subtractive hybridization library of monosex male gonads from monosex female gonads

text: 55 days post-fertilisation (dpf)

text: Oncorhynchus mykiss

text: Gonad

text: Oncorhynchus mykiss Undifferentiated gonad

characteristic: title


text: Bhabha Atomic Research Centre

text: live

text: Generic

text: Sesuvium plants at the seedling stage were stressed with 20% PEG-8000 for 0 to 16 hrs. The total RNA isolated from leaf tissues were used in RT-PCR. The PCR products were cloned and sequenced.

characteristic: description

text: seedling

text: E. coli

text: Leaf

text: Sesuvium portulacastrum

text: Leaf

text: Sesuvium portulacastrum PEG stress treated cDNA

text: Plasmid


text: M.S. Swaminathan Research Foundation

text: live

text: Generic

text: Fresh whole thallus of Roccella montagnei was sampled and total RNA isolated by a Lithium chloride based cold extraction buffer procedure. Ist and IInd strand cDNA was synthesised according to the manufacturerÕs instructions (Creator SMART cDNA library construction kit, BD Biosciences). The cDNA was then size-fractionated and directionally cloned between the 5' Sfi IA and 3' Sfi IB sites of pDNR_LIB vector. Single-pass sequencing from the 5' end of the ESTs was performed using pDNR_LIB specific forward primer in a automated sequencer (ABI 3130, Applied Biosystems). All the sequenced ESTs were screened for vector backbone contamination using the Vecscreen function. Their putative function was determined by performing the TBLASTX (nr/nt, nucleotide collection) algorithms using their default parameters.

text: Roccella montagnei

text: Sfi I A

text: Sfi I B

text: Vadanemmelli

text: Whole thallus

text: Roccella montagnei, natural thallus cDNA library

characteristic: vector


text: Cardiff University

text: live

text: Generic

text: Bowles Mauve

characteristic: cultivar

text: Erysimum linifolium

text: petal

text: Erysimum linifolium petal


text: Cardiff University

text: live

text: Generic

text: Bowles Mauve

text: Erysimum linifolium

text: leaf

text: Erysimum linifolium leaf


text: Nanjing Forestry University

text: live

text: Generic

text: Flowering related transcription factors of Lycoris longituba

text: floral organ

text: Lycoris longituba

text: hermaphrodite

characteristic: sex

text: floral organ

text: cDNA library of Lycoris longituba

text: plasmid

text: BlueScript SK


characteristic: External Id

text: Liaoning Ocean and Fisheries Science Research Institute

text: live

text: Generic

text: E. coli

text: hepatopancreas

text: Meretrix meretrix

text: Sfi IA

text: Sfi IB

text: hepatopancreas

text: hard clam SMART cDNA library from hepatopancreas

text: pDNR-LIB


characteristic: INSDC center name

text: live

text: Generic

text: E. coli

text: intestine

text: Meretrix meretrix

text: Sfi IA

text: Sfi IB

text: intestine

text: hard clam SMART cDNA library from intestine

text: pDNR-LIB


characteristic: INSDC first public

text: live

text: Generic

text: E. coli

text: muscle

text: Meretrix meretrix

text: Sfi IA

text: Sfi IB

text: muscle

text: hard clam SMART cDNA library from muscle

text: pDNR-LIB


text: Australian Institute of Marine Science

characteristic: INSDC last update

text: live

text: Generic

text: A suppression-subtractive hybridization PCR (SSH-PCR) library of differentially expressed genes obtained from the holobiome of high- and low-light exposed fragments of a colony of the coral A. microphthalma was created for sequencing and gene annotation.

text: Acropora microphthalma

text: Metagenomic library from Great Barrier Reef field experiment


text: Pharmaceutical Sciences, Aristorle University of Thessaloniki

text: live

text: Generic

text: murine erythroleukemia

characteristic: cell type

characteristic: cell-line

text: Mus musculus

characteristic: strain

text: Mus musculus erythroleukemia cell line MEL-745PC-A


text: Florida Institute of Technology

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: adult

characteristic: dev-stage

text: Aplysia californica

text: neural

text: Aplysia californica Adult Neural pSMART cDNA Library


text: Florida Institute of Technology

text: live

text: Generic

text: egg

text: Aplysia californica

characteristic: organism

text: Aplysia californica Egg Phase pSMART cDNA Library


text: Florida Institute of Technology

text: live

text: Generic

text: juvenil

text: Aplysia californica

text: Aplysia californica Juvenile Phase pSMART cDNA Library

characteristic: title


text: Florida Institute of Technology

text: live

text: Generic

text: Metamorphic

text: Aplysia californica

text: Aplysia californica Metamorphic Phase pSMART cDNA Library


text: Florida Institute of Technology

text: live

text: Generic

text: Veliger

text: Aplysia californica

text: Aplysia californica Veliger Phase pSMART cDNA Library


text: University of Minnesota

text: live

text: Generic

text: Skunk #1

text: Cannabis sativa plants were grown and processed in a DEA licensed and secured laboratory. Glands were removed from female inflorescences by vortexing, selected by sieving through a screen mesh, and collected by centrifugation. Total RNA was isolated from the glands using the Qiagen RNAeasy Plant Mini Kit (Qiagen Inc.-USA, Valencia, CA). The cDNA library from Cannabis trichomes was constructed using the Creator Smart cDNA library construction kit (Clontech, Palo Alto, CA, USA) following the manufacturer's protocol for total RNA.

characteristic: description

text: various developmental stages

text: Cannabis sativa

text: Sfi IA (GGCCATTACGGCC)

text: Sfi IB (GGCCGCCTCGGCC)

text: Glandular trichome from female inflorescences

characteristic: tissue-type

text: Cannabis sativa (marijuana cultivar Skunk #1) glandular trichomes isolated from female inflorescences

text: pDNR-LIB


text: Indian Institute of Chemical Biology (A Unit of Council of Scientific and Industrial Research)

text: live

text: Generic

text: According to the instructions given in Clontech Manual on PCR Select cDNA Subtraction Kit (Cat. No. 637401; Clontech Lab Inc, USA)

text: Vegetative stage

text: Escherichia coli

characteristic: lab-host

text: Leaf

characteristic: organ

text: Nicotiana tabacum

text: EcoRI

text: EcoRI

text: Transgenic tobacco leaf SSH cDNA library

text: pGEM-T Easy

characteristic: vector


characteristic: External Id

text: Institute of Environmental Science and Technology, Zhejiang University

characteristic: INSDC center name

text: live

text: Generic

text: Elsholtzia splendens

text: roots

text: Copper regulated fragments in Elsholtzia splendens roots

text: pMDTM18-T vector


text: Washington University School of Medicine

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: The library was constructed by Claire Murphy and Dr. James McCarter at Washington University, St. Louis. The cDNA was made by using Dynabead oligo-dT priming (Dynal). PCR based library using a modified protocol from the SMART PCR cDNA Synthesis Kit from Clontech. Directionally cloned into the UDG sites of pAMP1. Three day old female and male adults were collected from infected rats and provided by Dr. Judith Appleton of Cornell University, Ithaca, NY (jaa2@cornell.edu).

text: adult

text: Trichinella spiralis

text: NotI

text: SalI

text: mixed

characteristic: sex

text: Trichinella spiralis adult pAMP1 v1

text: plasmid (ampicillin resistant)

text: pAMP1 (Gibco)


text: Genetique Animale, Institut National Recherche Agronomique

text: live

text: Generic

text: Comparaison between granulosa cells from little/medium/large follicle by Suppression Substractive Hybridization.

text: little-medium-large follicle

text: Sus scrofa

text: Ovarian Follicle (granulosa)

text: Sus scrofa cDNA library (scaj)

text: pGEM-T Easy Sytem I


text: National Veterinary Research and Quarantine Services

text: live

text: Generic

text: tachyzoite

text: Toxoplasma gondii

text: ATCC: 40615

characteristic: strain

text: Toxoplasma gondii tachyzoite stage cDNA


text: National Taiwan University

text: live

characteristic: INSDC status

text: Generic

text: The subtractive cDNA library was constructed.

text: Taiwania cryptomerioides

text: leaf and flower

text: The subtractive cDNA library of T. cryptomerioides

text: pGEM-T Easy


text: Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences

text: live

text: Generic

characteristic: NCBI submission model

text: The conidia of strain Pm36 were inoculated in Sabouraud dextrose broth for 3 days. RNA was extracted and a cDNA library was constructed using the vector pTriplEx2.

text: Cordyceps militaris

text: mycelium

text: LM (for liquid mycelia)


text: live

text: Generic

characteristic: NCBI submission package

text: The conidia of strain Pm36 were used to inject the day 5 pupae of silkworm Bombyx mori and maintained under moisturized conidition. The fruit bodies were induced and cut for RNA isolation to generate the cDNA library using the vector pTriplEx2.

text: Cordyceps militaris

text: fruit body

text: PF (pupae fruit body)


text: live

text: Generic

text: The rice medium was inoculated with conidia and incubated until fruit body maturation. The stromata were collected for RNA isolation to construct the cDNA library using the vector pTriplEx2.

text: Cordyceps militaris

text: fruit body

text: RF (for rice medium fruit body)


text: live

text: Generic

text: The yellowish organge mycelial mats formed before the production of stromata were collected for RNA isolation to construct the cDNA library using the vector pTriplEx2.

text: Cordyceps militaris

text: mycelia

text: RM (for rice medium mycelia)


text: Institute of Forest Genetics and Tree Breeding

text: live

text: Generic

text: Total RNA was isolated from calli tissues of Casuarina equisetifolia elicited with cell wall components of the pathogen Trichosporium vesiculosum for 48 hours and reverse transcribed to cDNA. Transcript profiling of elicitor treated and untreated cDNA was conducted using arbitrary and anchored primer pairs and amplicons expressed differentially in treated cDNA pool were excised, reamplified, cloned and sequenced.

text: 48 hours post elicitation with cell wall components from pathogen

characteristic: dev-stage

text: CB5A Ampicillin resistant

text: Casuarina equisetifolia subsp. equisetifolia

text: EcoRI

text: HindIII

text: Needle

text: Casuarina equisetifolia 48 hours post elicitation with pathogen cell wall components

text: Plasmid


text: live

text: Generic

text: Total RNA was isolated from calli tissues of Casuarina equisetifolia elicited with cell wall components of the pathogen Trichosporium vesiculosum for 48 hours and reverse transcribed to cDNA. Transcript profiling of elicitor treated and untreated cDNA was conducted using gene specific primer pairs and amplicons expressed differentially in treated cDNA pool was excised, reamplified, cloned and sequenced.

text: CB5A Ampicillin resistant

characteristic: organism

text: EcoRI

text: HindIII

text: Needle

text: Casuarina equisetifolia 48 hours post elicitation with pathogen cell wall components (specific primers)

text: Plasmid


text: live

text: Generic

text: Total RNA was isolated from calli of Casuarina equisetifolia elicited with cell wall components of the pathogen Trichosporium vesiculosum for 48 hours and reverse transcribed to cDNA. Transcript profiling of elicitor treated and untreated cDNA was conducted using aribitrary and gene specific primers. Differentially expressed transcripts were sequenced and the data was used to generate specific primers for RACE using 3' full RACE core kit from Takara Bio Inc. Amplicons generated using RACE primers were cloned and sequenced .

text: CB5A Ampicillin resistant

text: EcoRI

text: HindIII

text: Needle

text: Casuarina equisetifolia 48 hours post elicitation with pathogen cell wall components (RACE)

characteristic: title

text: Plasmid


text: Kyungpook National University

text: live

text: Generic

text: coelomic cell

characteristic: cell type

text: Eisenia andrei

text: hermaphrodite

text: Cold shock Eisenia andrei coelomic library

text: Plasmid

text: pGEM-T Easy


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected >2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed at DOE Joint Genome Institute (Walnut Creek, CA).

characteristic: description

text: Tremella mesenterica

text: SalI

text: NotI

text: ATCC 24925

text: CCOI Tremella mesenterica jelly fungus (H)


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected 0.5-2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed at DOE Joint Genome Institute (Walnut Creek, CA).

characteristic: lab-host

text: Tremella mesenterica

text: SalI

text: NotI

text: ATCC 24925

text: CCON Tremella mesenterica jelly fungus (L)


text: DOE Joint Genome Institute

text: live

text: Generic

text: vegetative mycelia

text: Thermothielavioides terrestris

text: SalI

text: NotI

text: NRRL 8126

text: whole

characteristic: tissue-type

text: CCPH Thielavia terrestris pooled RNA from cells grown in MY50 (rich medium) and minimal medium with Arbocel (cellulose) - L


text: DOE Joint Genome Institute

text: live

text: Generic

text: vegetative mycelia

text: Thermothielavioides terrestris

text: SalI

text: NotI

text: NRRL 8126

text: whole

text: CCPI Thielavia terrestris pooled RNA from cells grown in MY50 (rich medium) and minimal medium with Arbocel (cellulose) - H

characteristic: vector


characteristic: External Id

text: Faculty of sciences Sfax, Tunisia

text: live

text: Generic

text: Deglet Noor

characteristic: cultivar

text: A subtractive suppression hybridization cDNA library was generated from brittle leaf disease affected leaves of Phoenix dactylifera (second stage of the disease). Affected cDNAs (Tester) was subtracted by control cDNA (Driver) using clontech PCR-Select cDNA subtraction kit (CLONTECH;k1804-1 ). The subtracted cDNAs were directly inserted in a T/A cloning vector (pGEMTeasy, PROMEGA). All sequenced ESTs are verified by differential screening using labeled probes from either driver or tester population.

text: Leaf

characteristic: organ

text: Phoenix dactylifera

text: EcoR I

text: EcoR I

text: SSH Library of Brittle Leaf Disease Affected date palm leaves

text: Plasmid

text: pGEM-T easy Vector (Promega)


text: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: The mRNA was isolated from the adult worms of Schistosoma japonicum and then the ds cDNA was synthesized by reverse transcription. The directional EcoRI/ HindIII linkers were ligated into the ends of ds cDNA and digested with EcoRI and HindIII, which resulted in ds cDNA with EcoRI and HindIII adhering ends. The digested ds cDNA fragments longer than 300bp in length were fractionated and ligated into the T7 Select 10-3b vector with EcoRI and HindIII adhering ends. After packaging in vitro, the recombinant T7 Select 10-3b vector was transformed into BLT5403 to construct the T7 phage display cDNA library. The primary library contained 4.98x106 clones, and the titer of amplified library was 3.85x1011pfu/mL. The PCR identification result of 96 clones picked at random showed that 93.8% clones of the library were recombinant, in which 95.6% clones contained the cDNA fragments longer than 300bp.

text: Adult worm

text: Schistosoma japonicum

text: Male and female

text: Chinese mainland

text: Whole body

text: T7 phage display cDNA library from worms of Schistosoma japonicum

text: Phage

text: T7 Select 10-3b vector


text: Federal University of Parana

text: live

characteristic: INSDC status

text: Generic

text: Loxosceles laeta

text: venom glands

text: Loxosceles laeta venom gland cDNA


text: Federal University of Parana

text: live

text: Generic

characteristic: NCBI submission model

text: Loxosceles gaucho

text: venom glands

text: Loxosceles gaucho venom gland cDNA


text: Genoscope - Centre National de Sequencage

text: live

text: Generic

characteristic: NCBI submission package

text: EST (SSH)

text: schizont

text: Plasmodium falciparum

characteristic: strain

text: Plasmodium falciparum FcB1 schizont


text: Rutgers, the State University of New Jersey

text: live

text: Generic

text: ipt transgenic

text: Library was prepared from the subtracted cDNA population extracted from the shoot of ipt transgenic creeping bentgrass (Agrostis stolonifera) vs. null-transformation control plant treated at 35C for 10 days, then cloned into TOPO TA cloning vector.

text: Agrostis stolonifera

characteristic: organism

text: shoot

text: HSP-ipt transgenic creeping bentgrass subtraction library 1

text: TA cloning vector

text: TOPO pCR2.1


text: live

text: Generic

text: ipt transgenic

text: Library was prepared from the subtracted cDNA population extracted from the shoot of ipt transgenic creeping bentgrass (Agrostis stolonifera) vs. null-transformation control plant treated at 35C for 2 days, then cloned into TOPO TA cloning vector.

text: Agrostis stolonifera

text: shoot

text: HSP-ipt transgenic creeping bentgrass subtraction library 2

characteristic: title

text: TA cloning vector

text: TOPO pCR2.1


text: live

text: Generic

text: ipt transgenic

text: Library was prepared from the subtracted cDNA population extracted from the root of ipt transgenic creeping bentgrass (Agrostis stolonifera) vs. null-transformation control plant treated at 35C for 10 days, then cloned into TOPO TA cloning vector.

text: Agrostis stolonifera

text: root

text: HSP-ipt transgenic creeping bentgrass subtraction library 3

text: TA cloning vector

text: TOPO pCR2.1


text: live

text: Generic

text: ipt transgenic

text: Library was prepared from the subtracted cDNA population extracted from the root of ipt transgenic creeping bentgrass (Agrostis stolonifera) vs. null-transformation control plant treated at 35C for 2 days, then cloned into TOPO TA cloning vector.

text: Agrostis stolonifera

text: root

text: HSP-ipt transgenic creeping bentgrass subtraction library 4

text: TA cloning vector

text: TOPO pCR2.1


text: Dana-Farber Cancer Institute

text: live

text: Generic

text: Chlamydomonas reinhardtii (CC-503 strain), reverse transcribed RNA library from cells grown in liquid culture under constant light. pDONR223 is a Gateway Vector.

text: E. coli DH5-alpha

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii (CC-503 strain) cells grown in liquid culture under constant light

text: Plasmid


text: The Sainsbury Laboratory

text: live

text: Generic

text: Phytophthora infestans

text: BamHI

text: Phytophthora infestans T30-4 NORMALIZED

text: Plasmid

text: pFLCIII-cDNA


text: Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences

text: live

text: Generic

text: microspore

characteristic: cell type

text: Different microspores samples induced by different pretreatments (including no-pretreatment, heat shock for 1 day, cold pretreatment for 1 day, the combination of cold pretreatment for 1 day and heat shock for 1 day) and then cultured at 25 degrees centigrade for 0-3 days were used to analyze the differential gene expression by differential display experiments in broccoli. Some differentially expressed TDFs were got. All sequences have been trimmed to remove vector sequences and low quality regions.

text: cultured for 0-3 days

characteristic: dev-stage

text: Brassica oleracea var. italica

text: Brassica oleracea var. italica microspore Library


text: live

text: Generic

text: microspore

text: Zhonggan 11

text: Different microspores samples induced by different pretreatments (including no-pretreatment and heat shock for 1 day) and then cultured at 25 degrees centigrade for 0-3 days were used to analyze the differential gene expression by differential display experiments in cabbage. Some differentially expressed TDFs were got. All sequences have been trimmed to remove vector sequences and low quality regions.

characteristic: description

text: cultured for 0-3 days

text: Brassica oleracea var. capitata

text: Brassica oleracea var. capitata microspore Library


text: University of Maryland at Baltimore

text: live

text: Generic

text: Epithelial

text: PCR-based cDNA library made with SMART cDNA library construction kit from Clontech

text: Adults fed for 4 days

text: Midgut

text: Ctenocephalides felis

text: Female

characteristic: sex

text: Midgut

characteristic: tissue-type

text: Adult Ctenocephalides felis 4 day fed midgut library (CFGF)

text: Phage

text: Lambda TriplEx2


characteristic: External Id

text: University of Maryland at Baltimore

text: live

text: Generic

text: Epithelial

text: Adults fed for 4 days on Rickettsia typhi infected bloodmeal

text: Midgut

text: Ctenocephalides felis

text: Female

text: Midgut

text: Midgut library from adult Ctenocephalides felis 4 days on Rickettsia typhi infected bloodmeal (CFGFI)

text: Phage

text: Lambda TriplEx2

characteristic: vector


text: Third Institute of Oceanography, SOA

text: live

text: Generic

text: Perinereis nuntia

text: Subtractive cDNA Library of Perinereis nuntia


text: Northwest A&F University

text: live

text: Generic

characteristic: cultivar

text: seedling stage

text: Triticum aestivum

text: wheat leaves

text: cDNA library of a compatible interaction between stripe rust (Puccinia striiformis) and wheat


text: Rubber Research Institute of India

characteristic: INSDC center name

text: live

text: Generic

text: This library contains transcripts specific to TPD condition of Hevea brasiliensis. The mRNA from latex of TPD affected trees of Hevea had been subtracted with mRNA from latex of healthy trees by using Clontech PCR Select Subtraction Kit, USA and the PCR amplified fragments of subtracted cDNA were cloned into the PCR-TRAP Vector (GenHunter, USA). Sequencing was carried out by Macrogen, Korea. The sequences may contain flanked sequences of Nested PCR Primer 1 (TCGAGCGGCCGCCCGGGCAGGT) and Nested PCR Primer 2R (AGCGTGGTCGCGGCCGAGGT) of Clontech PCR Select Subtraction Kit.

text: JM109 cells

characteristic: lab-host

text: Hevea brasiliensis

text: latex

text: Transcripts specific to latex of TPD affected trees of Hevea brasiliensis

text: plasmid

text: PCR_TRAP cloning vector


text: Rubber Research Institute of India

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: This library contains transcripts specific to healthy trees of Hevea. The mRNA from latex of healthy trees had been subtracted with mRNA from latex of TPD affected trees by using Clontech PCR Select Subtraction Kit, USA and the PCR amplified fragments of subtracted cDNA were cloned into the PCR-TRAP Vector (GenHunter, USA). Sequencing was carried out by Macrogen, Korea. The sequences may contain flanked sequences of Nested PCR Primer 1 (TCGAGCGGCCGCCCGGGCAGGT) and Nested PCR Primer 2R (AGCGTGGTCGCGGCCGAGGT) of Clontech PCR Select Subtraction Kit.

text: JM109 cells

text: Hevea brasiliensis

text: latex

text: Transcripts specific to latex of healthy trees of Hevea brasiliensis

text: plasmid

text: PCR_TRAP cloning vector


text: Rubber Research Institute of India

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: This library contains transcripts specific to TPD condition of Hevea brasiliensis. The mRNA from TPD affected bark had been subtracted with mRNA from bark of healthy trees by using Clontech PCR Select Subtraction Kit, USA and the PCR amplified fragments of subtracted cDNA were cloned into the pGEM-T Easy Vector (Promega, USA). Sequencing was carried out by Macrogen, Korea. The sequences may contain flanked sequences of Nested PCR Primer 1 (TCGAGCGGCCGCCCGGGCAGGT) and Nested PCR Primer 2R (AGCGTGGTCGCGGCCGAGGT) of Clontech PCR Select Subtraction Kit.

text: JM109 cells

text: Hevea brasiliensis

text: bark (TPD affected)

text: TPD responsive transcripts of Hevea brasiliensis (TPD affected bark)

text: plasmid

text: pGEM-T Easy vector


text: Rubber Research Institute of India

text: live

text: Generic

characteristic: NCBI submission package

text: This library contains transcripts specific to healthy trees of Hevea brasiliensis. The mRNA from healthy trees were subtracted with mRNA from TPD affected tree by using Clontech PCR Select Subtraction Kit, USA and the PCR amplified fragments of subtracted cDNA were cloned into the pGEM-T Easy Vector (Promega, USA).Sequencing was carried out by Macrogen, Korea. The sequences may contain flanked sequences of Nested PCR Primer 1 (TCGAGCGGCCGCCCGGGCAGGT) and Nested PCR Primer 2R (AGCGTGGTCGCGGCCGAGGT) of Clontech PCR Select Subtraction Kit.

text: JM109 cells

text: Hevea brasiliensis

text: bark (healthy tree)

text: Transcripts of bark tissues of healthy trees

text: plasmid

text: pGEM-T Easy vector


text: Rubber Research Institute of India

text: live

text: Generic

text: This library contains transcripts specific to TPD condition of Hevea brasiliensis. The mRNA from TPD affected bark portion of partially affected tree (50 %) had been subtracted with mRNA of bark tissue obtained from the healthy portion of the same tree using Clontech PCR Select Subtraction Kit, USA and the PCR amplified fragments of subtracted cDNA were cloned into the pGEM-T Easy Vector (Promega, USA). Sequencing was carried out by Macrogen, Korea. The sequences may contain flanked sequences of Nested PCR Primer 1 (TCGAGCGGCCGCCCGGGCAGGT) and Nested PCR Primer 2R (AGCGTGGTCGCGGCCGAGGT) of Clontech PCR Select Subtraction Kit.

text: JM109 cells

text: Hevea brasiliensis

characteristic: organism

text: bark tissue from TPD affected portion of 50 % TPD tree

text: TPD responsive transcripts of Hevea brasiliensis (partially affected tree)

text: plasmid

text: pGEM-T Easy vector


text: Rubber Research Institute of India

text: live

text: Generic

text: This library contains transcripts specific to healthy bark portion of a partially TPD affected tree (Hevea brasiliensis). The mRNA from healthy portion of partially affected tree (50 %)had been subtracted with mRNA of bark tissue obtained from the TPD affected portion of the same tree using Clontech PCR Select Subtraction Kit, USA and the PCR amplified fragments of subtracted cDNA were cloned into the pGEM-T Easy Vector (Promega, USA). Sequencing was carried out by Macrogen, Korea. The sequences may contain flanked sequences of Nested PCR Primer 1(TCGAGCGGCCGCCCGGGCAGGT) and Nested PCR Primer 2R (AGCGTGGTCGCGGCCGAGGT) of Clontech PCR Select Subtraction Kit.

text: JM109 cells

text: Hevea brasiliensis

text: bark tissue from healthy portion of 50 % TPD tree

text: transcripts from healthy bark tissue of Hevea brasiliensis

characteristic: title

text: plasmid

text: pGEM-T Easy vector


text: Northwest A & F University

text: live

text: Generic

text: cDNA library produced with oligo dT.

text: larva

text: gut

characteristic: organ

text: Mayetiola destructor

text: Biotype L

characteristic: strain

text: gut

text: Hessian fly larval gut


text: Qiqihar University

text: live

text: Generic

text: Grimmia pilifera was sampled after 5 d of dehydration before cDNA library construction.

characteristic: description

text: Grimmia pilifera

text: sfi

text: sfi

text: Gametophyte

text: Grimmia pilifera drought stress

text: Plasmid

text: pDNR-LIB


text: Institut National de la Recherche Agronomique

text: live

text: Generic

text: mixed

characteristic: dev-stage

text: multiple

text: Sus scrofa

text: mixed

text: Resequencing of clones from GPL3729 chip (scai)

text: multiple


text: live

text: Generic

text: mixed

text: multiple

text: Sus scrofa

text: mixed

characteristic: tissue-type

text: Resequencing of clones from GPL3729 chip (scbg)

text: multiple


characteristic: External Id

text: live

text: Generic

text: mixed

text: multiple

text: Bos taurus

text: mixed

text: Resequencing of clones from GPL3729 chip (scai) (Bos taurus)

text: multiple


text: Harbin Institute of Technology

text: live

text: Generic

text: Trichoderma asperellum

text: mycelium

text: cDNA Library of Trichoderma asperellum


text: Northwest Agriculture and Foresty University

text: live

text: Generic

text: Greenhouse grown plants. cDNA-AFLP technology.

text: Brassica rapa subsp. pekinensis

text: aborting bud

text: aborting bud in Chinese cabbage

characteristic: vector


text: University of Aberdeen

text: live

text: Generic

text: Normalised cDNA library prepared using cDNA from unfed adult Rhipicephalus sanguineus (mixed male and female) synganglia. pCMV SPORT 6.1 was the vector utilised. Not I and EcoRV were the endonucleases used.

text: unfed adult

text: synganglia

text: Rhipicephalus sanguineus

text: NotI

text: EcoRV

text: mixed male and female

characteristic: sex

text: Rhipicephalus sanguineus synganglion EST library

text: pCMVSPORT 6.1


text: Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences

characteristic: INSDC center name

text: live

text: Generic

text: microspore

characteristic: cell type

characteristic: cultivar

text: The sequences are the products of 3' RACE experiments, and came from broccoli microspores mRNA. All sequences have been trimmed to remove vector sequences and low quality regions.

text: cultured for 0-3 days

text: Brassica oleracea var. italica

text: Brassica oleracea var. italica microspore Library (3'RACE)


text: Westf. Wilhelms-Universtitaet Muenster

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: isolation_source: axenic culture

text: Claviceps purpurea

text: Claviceps purpurea 20.1 (axenic culture)


text: Westf. Wilhelms-Universtitaet Muenster

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: isolation_source: host ovaries 6-20 days post innoculation. host: Secale cereale.

text: Claviceps purpurea

text: Claviceps purpurea 20.1 (host ovaries)


text: DOE Joint Genome Institute

text: live

text: Generic

characteristic: NCBI submission package

text: In Vivo Conditions: grown under ambient rainfall. The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected >2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed and sequencing performed at the DOE Joint Genome Institute (Walnut Creek, CA).

text: booting stage

characteristic: lab-host

text: Avena barbata

characteristic: organism

text: SalI

text: NotI

text: leaf

text: CCOO Avena barbata leaf, grown under ambient rainfall (H)


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: grown under ambient rainfall. The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected 0.6-2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed and sequencing performed at the DOE Joint Genome Institute (Walnut Creek, CA).

text: booting stage

text: Avena barbata

text: SalI

text: NotI

text: leaf

text: CCOP Avena barbata leaf, grown under ambient rainfall (L)

characteristic: title


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: grown under high rainfall. The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected >2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed and sequencing performed at the DOE Joint Genome Institute (Walnut Creek, CA).

characteristic: description

text: booting stage

text: Avena barbata

text: SalI

text: NotI

text: leaf

text: CCOS Avena barbata leaf, grown under high rainfall (H)


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: grown under high rainfall. The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected 0.6-2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed and sequencing performed at the DOE Joint Genome Institute (Walnut Creek, CA).

text: booting stage

characteristic: dev-stage

text: Avena barbata

text: SalI

text: NotI

text: leaf

text: CCOT Avena barbata leaf, grown under high rainfall (L)


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: pooled from different levels of rain and nitrogen. The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected >2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed and sequencing performed at the DOE Joint Genome Institute (Walnut Creek, CA).

text: tillering stage

text: Avena barbata

text: SalI

text: NotI

text: leaf

characteristic: tissue-type

text: CCOU Avena barbata leaf, pooled from different levels of rain and nitrogen (H)


characteristic: External Id

text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: pooled from different levels of rain and nitrogen. The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 0.6-2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed and sequencing performed at the DOE Joint Genome Institute (Walnut Creek, CA).

text: tillering stage

text: Avena barbata

text: SalI

text: NotI

text: leaf

text: CCOW Avena barbata leaf, pooled from different levels of rain and nitrogen (L)


text: DOE Joint Genome Institute

text: live

text: Generic

text: tillering stage

text: Avena barbata

text: SalI

text: NotI

text: root

text: CCOX Avena barbata root, pooled from different levels of rain and nitrogen (H)

characteristic: vector


text: DOE Joint Genome Institute

text: live

text: Generic

text: tillering stage

text: Avena barbata

text: SalI

text: NotI

text: root

text: CCOY Avena barbata root, pooled from different levels of rain and nitrogen (L)


text: Dynamac Corporation c/o, US EPA

text: live

text: Generic

text: PCR-Select Suppression Subtractive Hybridization (Clontech)

text: Larvae

text: Diabrotica virgifera virgifera

text: Whole body

text: Western corn rootworm Subtractive cDNA library


text: Shanghai Jiao Tong University

text: live

text: Generic

text: SSH cDNA library was prepared from RNA of liquid static culture of mycelia as tester, and RNA of traditional shaking culture of mycelia as driver. The cDNA isolated with SSH were cloned in the vector PUC18. The inserts were amplified by PCR and the corresponding amplicons were blotted onto Hybond-N membranes. The up-regulated genes were identified by dot blot differential screening of clones.

text: Ganoderma lucidum

text: CCGMC 5.616 from China General Microbiological Fermentation Center

characteristic: strain

text: Mycelium

text: Ganoderma lucidum SSH up-regulated cDNA library during development


text: Northwest A & F University

characteristic: INSDC center name

text: live

text: Generic

text: Total RNA was extracted by Biozol from young spikes of thermosensitive male sterile wheat line YM3314 sowed in autumn (male sterile) and spring (male fertile), respectively. then reverse transcription was conducted by AMV reverse transcriptase; doublestrand cDNA was synthesized and subjected to SSH analysis by using PCR Select cDNA Subtraction Kit(Clontech company) with cDNA from male fertile spikes as tester and cDNA from male sterile spikes as driver. The PCR products were purified and cloned into PMD18-T vector and transformed to E coli strain DH5a, sequences were achieved using ABI PRISM 3730 from sangon,Shanghai,China.

text: monokaryotic stage

text: Triticum aestivum

text: young spike

text: Male fertile SSH-cDNA Library of young spike of YM type thermo-sensitive male-sterile wheat line during monokaryotic stage


text: Northwest A & F University

text: live

text: Generic

text: Total RNA was extracted by Biozol from young spikes of thermosensitive male sterile wheat line YM3314 sowed in autumn (male sterile) and spring (male fertile), respectively. then reverse transcription was conducted by AMV reverse transcriptase; doublestrand cDNA was synthesized and subjected to SSH analysis by using PCR Select cDNA Subtraction Kit(Clontech company) with cDNA from male sterile spikes as tester and cDNA from male fertile spikes as driver. The PCR products were purified and cloned into PMD18-T vector and transformed to E coli strain DH5a, sequences were achieved using ABI PRISM 3730 from sangon,Shanghai,China.

text: monokaryotic stage

text: Triticum aestivum

text: young spike

text: Male sterile SSH-cDNA Library of young spike of YM type thermo-sensitive male-sterile wheat line during monokaryotic stage


text: Forestry and Forest Products Research Institute

text: live

text: Generic

text: Fomitopsis palustris

text: NotI

text: SalI

text: NBRC 30339

text: Fomitopsis palustris cDNA library FP1

text: plasmid

text: pSPORTI


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: pool of mycelia grown in PDB liquid media (unstressed) and mycelia grown on cellophane overlaying solid PDA media (stressed). The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 2-8kb using 1.1% agarose gel electrophoresis, then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed and sequencing performed at the DOE Joint Genome Institute (Walnut Creek, CA).

text: Cryphonectria parasitica

text: SalI

text: NotI

text: mycelia

text: CCBH Cryphonectria parasitica pool of mycelia - H


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: pool of mycelia grown in PDB liquid media (unstressed) and mycelia grown on cellophane overlaying solid PDA media (stressed). The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 0.5-2kb using 1.1% agarose gel electrophoresis, then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed and sequencing performed at the DOE Joint Genome Institute (Walnut Creek, CA).

text: Cryphonectria parasitica

text: SalI

text: NotI

text: mycelia

text: CCBI Cryphonectria parasitica pool of mycelia - L


text: Federal University of Rio Grande do Sul

text: live

text: Generic

text: Sultanina

text: Representational Difference Analysis (RDA) was performed essentially as described by Hubank and Schatz (1994) and optimized by Pastorian et al. (2000). Double-stranded cDNA pools (approximately 2 microgram) were digested with MboI. Resulting products were purified using the GFX Kit (GE Healthcare) and ligated to adapters RBam12 (GATCCTCGGTGA) and RBam24 (AGCACTCTCCAGCCTCTCTCACCGAG). In order to provide sufficient amounts of starting material for RDA, adapter-bound cDNA products were diluted and amplified by PCR using RBAM24 as primer (25 cycles of denaturing at 95 oC for 45 s and annealing and extension at 70 oC for 3 min). Final PCR products were purified by GFX, resulting in the driver cDNA population. To generate the tester cDNA population, a small aliquot of the driver was completely digested with MboI to remove the RBam24 termini. After a new purification using GFX, the resulting product was ligated to the NBam12 (GATCCTCCCTCG) and NBam24 (AGGCAACTGTGCTATCCGAGGGAG) adapters. During three harvest seasons (2004/2005, 2006/2007 and 2007/2008), fruits from the same clonal plants were collected in five Developmental Stages (DS), i.e., from fruit-set (FS) to two, four, six and eight weeks after FS (DS2, DS4, DS6 and DS8). Total RNA samples were purified from first year's berries at FS, DS4 and DS8 and pooled in three ways for the production of three subtracted libraries. Equimolar cDNA quantities from DS4 and DS8 were pooled as drivers to subtract the FS tester cDNA population, producing the first subtracted library (L1). To perform the first round of hybridization and amplification, generating the first differential product (DP1), driver and tester populations were mixed in the 50:1 ratio in a 5 microliter reaction at 67 oC for 24 h, and amplified by PCR with NBam24 (7 cycles at 95 oC for 45 s and 70 oC for 3 min). PCR products were diluted and submitted to a new round of amplification to remove unwanted single-stranded cDNAs. After purification, DP1 products were digested with MboI to remove the NBam adapters before ligation to JBam12 (GATCCGTTCATG) and JBam24 (ACCGACGTCGACTATCCATGAACG) adapters. The second hybridization was performed at a 100:1 ratio. The second differential product (DP2) was obtained using the same procedure as for DP1, using JBam24 as primer instead of NBam24.

text: Fruit set (FS) - stage 7-71

text: Escherichia coli DH10B

text: Fruit

characteristic: organ

text: Vitis vinifera

text: EcoRI

text: EcoRI

text: Hermaphrodite (perfect flowers)

characteristic: sex

text: Skin, flesh and seed

text: Fruit set (FS) RDA subtracted library

text: Plasmid

text: pGEM-T Easy (Promega)


text: Federal University of Rio Grande do Sul

characteristic: INSDC first public

text: live

text: Generic

text: Sultanina

characteristic: cultivar

text: Developmental stage four (DS4)- stage 7-75

text: Escherichia coli DH10B

text: Fruit

text: Vitis vinifera

text: EcoRI

text: EcoRI

text: Hermaphrodite (perfect flowers)

text: Skin, flesh and seed

text: Developmental stage four (DS4) RDA subtracted library

text: Plasmid

text: pGEM-T Easy (Promega)


text: Federal University of Rio Grande do Sul

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Sultanina

text: Developmental stage 8 (DS8)- stage 8-81

text: Escherichia coli DH10B

text: Fruit

text: Vitis vinifera

text: EcoRI

text: EcoRI

text: Hermaphrodite (perfect flowers)

text: Skin, flesh and seed

text: Developmental stage eight (DS8) RDA subtracted library

text: Plasmid

text: pGEM-T Easy (Promega)


text: International Crops Research Institute for the Semi-Arid Tropics (ICRISAT)

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: cDNA library was constructed from root tissues of 25 days old chickpea seedling of ICC1882 genotype imposed to dehydration for a period of 7 days in greenhouse conditions. The cDNA fragments were cloned into pGEM-T Easy vector and subsequently sequenced by Sanger sequencing approach using M13 primers.

text: E. coli (DH5a)

text: Cicer arietinum

text: EcoRI

text: root

text: ICC1882 dehydration stressed root cDNA library

text: plasmid

text: pGEM-T Easy vector


text: live

text: Generic

text: cDNA library was constructed from root tissues of 25 days old chickpea seedling of ICC1882 genotype imposed to drought stress in field conditions. The cDNA fragments were cloned into pGEM-T Easy vector and subsequently sequenced by Sanger sequencing approach using M13 primers.

text: E. coli (DH5a)

characteristic: lab-host

text: Cicer arietinum

characteristic: organism

text: EcoRI

text: root

text: ICC1882 field drought stressed root cDNA library

text: plasmid

text: pGEM-T Easy vector


text: live

text: Generic

text: cDNA library was constructed from root tissues of 25 days old chickpea seedling of ICC1882 genotype imposed to slow drought (dry down) stress to allow gradual transpiration in greenhouse conditions. The cDNA fragments were cloned into pGEM-T Easy vector and subsequently sequenced by Sanger sequencing approach using M13 primers.

characteristic: description

text: E. coli (DH5a)

text: Cicer arietinum

text: EcoRI

text: root

text: ICC1882 Slow drought stressed root cDNA library

characteristic: title

text: plasmid

text: pGEM-T Easy vector


text: live

text: Generic

text: cDNA library was constructed from 25 days old chickpea seedling of ICC1882 genotype, treated with 1 mM PEG to induce drought stress. The cDNA fragments were cloned into pGEM-T Easy vector and subsequently sequenced by Sanger sequencing approach using M13 primers.

text: E. coli (DH5a)

text: Cicer arietinum

text: EcoRI

text: root

characteristic: tissue-type

text: ICC1882 PEG induced drought cDNA library

text: plasmid

text: pGEM-T Easy vector


characteristic: External Id

text: live

text: Generic

text: cDNA library was constructed from root tissues of 25 days old chickpea seedling of ICC4958 genotype imposed to dehydration for a period of 7 days in greenhouse conditions. The cDNA fragments were cloned into pGEM-T Easy vector and subsequently sequenced by Sanger sequencing approach using M13 primers.

text: E. coli (DH5a)

text: Cicer arietinum

text: EcoRI

text: root

text: ICC4958 dehydration stressed root cDNA library

text: plasmid

text: pGEM-T Easy vector


text: live

text: Generic

text: cDNA library was constructed from root tissues of 25 days old chickpea seedling of ICC4958 genotype imposed to drought stress in field conditions. The cDNA fragments were cloned into pGEM-T Easy vector and subsequently sequenced by Sanger sequencing approach using M13 primers.

text: E. coli (DH5a)

text: Cicer arietinum

text: EcoRI

text: root

text: ICC4958 field drought stressed root cDNA library

text: plasmid

text: pGEM-T Easy vector

characteristic: vector


text: live

text: Generic

text: cDNA library was constructed from root tissues of 25 days old chickpea seedling of ICC4958 genotype imposed to slow drought (dry down) stress to allow gradual transpiration in greenhouse conditions. The cDNA fragments were cloned into pGEM-T Easy vector and subsequently sequenced by Sanger sequencing approach using M13 primers.

text: E. coli (DH5a)

text: Cicer arietinum

text: EcoRI

text: root

text: ICC4958 Slow drought stresses root cDNA library

text: plasmid

text: pGEM-T Easy vector


text: live

text: Generic

text: cDNA library was constructed from root tissues of 25 days old chickpea seedling of ICC4958 genotype treated with 1 mM PEG to induce drought stress. The cDNA fragments were cloned into pGEM-T Easy vector and subsequently sequenced by Sanger sequencing approach using M13 primers.

text: E. coli (DH5a)

text: Cicer arietinum

text: EcoRI

text: root

text: ICC4958 PEG induced drought cDNA library

text: plasmid

text: pGEM-T Easy vector


text: International Crops Research Institute for the Semi-Arid Tropics (ICRISAT)

text: live

text: Generic

text: cDNA library was constructed from 25 days old chickpea seedling of ICCV2 genotype, treated with 80 mM NaCl for a period of 7 days in greenhouse conditions to induce salinity stress. The cDNA fragments were cloned into pGEM-T Easy vector and subsequently sequenced by Sanger sequencing approach using M13 primers.

text: E. coli (DH5a)

text: Cicer arietinum

text: EcoRI

text: root

text: ICCV2 Salinity-stressed chickpea root cDNA library

text: plasmid

text: pGEM-T Easy vector


text: live

text: Generic

text: cDNA library was constructed from 25 days old chickpea seedling of JG11 genotype, treated with 80 mM NaCl for a period of 7 days in greenhouse conditions to induce salinity stress. The cDNA fragments were cloned into pGEM-T Easy vector and subsequently sequenced by Sanger sequencing approach using M13 primers.

text: E. coli (DH5a)

text: Cicer arietinum

text: EcoRI

text: root

text: JG11 Salinity-stressed chickpea root cDNA library

text: plasmid

text: pGEM-T Easy vector


text: The Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences

text: live

text: Generic

text: Total RNA was extracted from the control group and the treated group of Dendrobium nobile leaf and stem induced by AR-18, one mycorrhizal fungus, respectively. DDRT-PCR, reverse Northern blot and Southern blot analysis were used to isolate and identify genes whose transcription were altered in cultured D. nobile plants that were treated with AR-18. The differentially displayed cDNAs were cloned directionally into the pBluescript SK(+/-) plasmid.

text: seedling

characteristic: dev-stage

text: Dendrobium nobile

text: Dendrobium nobile genes in response to mycorrhizal fungus

text: Plasmid

text: pBluescript SK(+/-)


text: University of Florida

characteristic: INSDC center name

text: live

text: Generic

text: Total RNA was isolated and cDNA synthesized. Adaptors were ligated onto the cDNA and then amplified. This product was then cloned and traditional sanger sequencing generated.

text: Adult

text: Trichoplax adhaerens

text: Whole animal

text: Trichoplax adhaerens EST Library


text: University of Blaise Pascal

text: live

text: Generic

text: franquette

text: adult

text: E. coli DH5a thermocompetent cells

text: leaf

characteristic: organ

text: Juglans regia

text: light-regulated genes in Walnut leaves

text: pGEM-T easy


text: live

text: Generic

text: Total RNA was extracted from the control group and the treated group of Anoectochilus roxburghii leaf and stem induced by AR-18, one mycorrhizal fungus, respectively. DDRT-PCR and reverse Northern blot analysis were used to isolate and identify genes whose transcription were altered in tissue cultured seedlings of Anoectochilus roxburghii plants that were treated with AR-18. The differentially displayed cDNAs were cloned directionally into the pGEM-T easy plasmid.

text: Tissue cultured seedling

text: Anoectochilus roxburghii

text: Anoectochilus roxburghii genes in response to mycorrhizal fungus

text: Plasmid

text: pGEM-T easy


text: Instituto Valenciano de Investigaciones Agrarias (IVIA)

text: live

text: Generic

text: Enriched in dormant bud

text: adult

text: Prunus persica

text: hermaphrodite

characteristic: sex

text: Springlady

characteristic: strain

text: flower bud

text: DR

text: Plasmid

text: pGEM-T Easy


characteristic: INSDC first public

text: live

text: Generic

text: Enriched in dormancy released bud

text: adult

text: Prunus persica

text: hermaphrodite

text: Springlady

text: flower bud

text: RD

text: Plasmid

text: pGEM-T Easy


characteristic: INSDC last update

text: live

text: Generic

text: Enriched in 'Zincal 5' dormancy released bud

text: adult

text: Prunus persica

text: hermaphrodite

text: Zincal 5

text: flower bud

text: ZS

text: Plasmid

text: pGEM-T Easy


text: live

characteristic: INSDC status

text: Generic

text: Enriched in 'Springlady' dormancy released bud

text: adult

text: Prunus persica

text: hermaphrodite

text: Springlady

text: flower bud

text: SZ

text: Plasmid

text: pGEM-T Easy


text: University of Bologna

text: live

text: Generic

characteristic: NCBI submission model

text: Farold 40 (Old Home x Farmingdale)

characteristic: cultivar

text: E.Coli strain XL-1 blue

text: Pyrus communis

text: leaves

text: Sodium transporters in pear

text: pGEM-T Easy


text: University of Bologna

text: live

text: Generic

characteristic: NCBI submission package

text: EMC

text: E.Coli strain XL-1 blue

characteristic: lab-host

text: Cydonia oblonga

text: leaves

text: Sodium transporters in quince

text: pGEM-T Easy


text: Genoscope - Centre National de Sequencage

text: live

text: Generic

text: Ectocarpus siliculosus

characteristic: organism

text: immatures sporophytes not-normalised


text: Universitaet Hamburg, Biozentrum Klein Flottbek

text: live

text: Generic

text: Hybrid UH301xUH005

text: SMART cDNA synthesis and PCR-Select cDNA subtraction (Clontech) of Hybrid cDNA with parental line cDNA, PCR products T/A cloned

characteristic: description

text: 6 days after pollination

text: E. coli, XL1 Blue

text: Zea mays

text: endosperm

characteristic: title

text: pGEM T-Easy, Promega


characteristic: External Id

text: Uppsala University

text: live

text: Generic

text: hemocyte

characteristic: cell type

text: Pacifastacus leniusculus

text: Pacifastacus leniusculus hemocyte


text: Embrapa - Genetic Resources and Biotechnology, Brasilia-DF Brazil

text: live

text: Generic

text: Most parenchyma

text: RNA extraction: Total RNA was extracted from cassava storage roots using phenol chloroform procedure. Construction of subtractive cDNA library: Polyadenylated RNA was purified from total RNA by using the mRNA Purification kit (Pharmacia). Double-stranded cDNAs were synthesized and subtractive PCR was conducted using the PCR-Select cDNA Subtraction Kit (Clontech). cDNAs were synthesized separately with 2ug of polyA+ RNA from the testers (CAS36.04) and the driver (IAC 12.829) and separately digested with RsaI. The tester sample then was divided into two populations and each one ligated with adaptor 1 or 2R. Each tester population was hybridized separately with excess driver cDNA (33-fold). The two hybridization reactions were combined (with additional driver cDNA) for a second subtractive hybridization. The hybridizations led to equalization and enrichment of differentially expressed sequences. Finally, the differentially expressed cDNAs were amplified by PCR and cloned into the pGEM-Teasy vector (Promega). cDNA clone sequence: Single bacterial colonies of the result of all the libraries were inoculated in 96-well microtiter plates containing LB and ampicillin (100 mg/L) and allowed to grow overnight with shaking at 37oC (18 hr at 320 RPM). The DNA was purified by standard alkaline lysis method with one modification at the end of the procedure, where the supernatant was passed through a multi-screen filter (Millipore) prior to DNA precipitation. The purified DNA was ressuspended in water. The sequencing reactions were performed with 200ng of DNA using standard protocols of the ThermoSequenase II dye terminator cycle sequencing kit (Amersham-Pharmacia Biotech) using universal M13 forward and reverse primers and the capillary sequencer MegaBACE 1000. The samples were electro injected by 2 KV for 100 seconds and the fragments were separated at 9 KV for 100 minutes. Sequence analysis of ESTs: The genome tool and facilities from EMBRAPA (www.genoma.embrapa.br) were used to test for sequence read quality by PHRED (Ewing et al 1998), sequences portion of poor quality were trimmed and low quality sequence (PHRED >20) removed from further analysis. Vector, primers and adaptors sequences were automatically trimmed from the high quality sequences. Only ESTs whose sequences exceed 250bp after trimming were considered for further analyses and submition.

text: Advanced secondary growth

characteristic: dev-stage

text: E. coli

text: Storage root

text: Manihot esculenta

text: Storage root

characteristic: tissue-type

text: MAAC

text: Plasmid

characteristic: vector


characteristic: INSDC center name

text: live

text: Generic

text: Most parenchyma

text: RNA extraction: Total RNA was extracted from cassava storage roots using phenol chloroform procedure. Construction of subtractive cDNA library: Polyadenylated RNA was purified from total RNA by using the mRNA Purification kit (Pharmacia). Double-stranded cDNAs were synthesized and subtractive PCR was conducted using the PCR-Select cDNA Subtraction Kit (Clontech). cDNAs were synthesized separately with 2ug of polyA+ RNA from the testers (CAS36.01) and the driver (IAC 12.829) and separately digested with RsaI. The tester sample then was divided into two populations and each one ligated with adaptor 1 or 2R. Each tester population was hybridized separately with excess driver cDNA (33-fold). The two hybridization reactions were combined (with additional driver cDNA) for a second subtractive hybridization. The hybridizations led to equalization and enrichment of differentially expressed sequences. Finally, the differentially expressed cDNAs were amplified by PCR and cloned into the pGEM-Teasy vector (Promega). cDNA clone sequence: Single bacterial colonies of the result of all the libraries were inoculated in 96-well microtiter plates containing LB and ampicillin (100 mg/L) and allowed to grow overnight with shaking at 37oC (18 hr at 320 RPM). The DNA was purified by standard alkaline lysis method with one modification at the end of the procedure, where the supernatant was passed through a multi-screen filter (Millipore) prior to DNA precipitation. The purified DNA was ressuspended in water. The sequencing reactions were performed with 200ng of DNA using standard protocols of the ThermoSequenase II dye terminator cycle sequencing kit (Amersham-Pharmacia Biotech) using universal M13 forward and reverse primers and the capillary sequencer MegaBACE 1000. The samples were electro injected by 2 KV for 100 seconds and the fragments were separated at 9 KV for 100 minutes. Sequence analysis of ESTs: The genome tool and facilities from EMBRAPA (www.genoma.embrapa.br) were used to test for sequence read quality by PHRED (Ewing et al 1998), sequences portion of poor quality were trimmed and low quality sequence (PHRED >20) removed from further analysis. Vector, primers and adaptors sequences were automatically trimmed from the high quality sequences. Only ESTs whose sequences exceed 250bp after trimming were considered for further analyses and submition.

text: Advanced secondary growth

text: E. coli

text: Storage root

characteristic: organ

text: Manihot esculenta

text: Storage root

text: MAGL

text: Plasmid


characteristic: INSDC first public

text: live

text: Generic

text: Most parenchyma

text: IAC 12.829

text: RNA extraction: Total RNA was extracted from cassava storage roots using phenol chloroform procedure. Construction of subtractive cDNA library: Polyadenylated RNA was purified from total RNA by using the mRNA Purification kit (Pharmacia). Double-stranded cDNAs were synthesized and subtractive PCR was conducted using the PCR-Select cDNA Subtraction Kit (Clontech). cDNAs were synthesized separately with 2ug of polyA+ RNA from the testers (IAC 12.829) and the driver (CAS36.01) and separately digested with RsaI. The tester sample then was divided into two populations and each one ligated with adaptor 1 or 2R. Each tester population was hybridized separately with excess driver cDNA (33-fold). The two hybridization reactions were combined (with additional driver cDNA) for a second subtractive hybridization. The hybridizations led to equalization and enrichment of differentially expressed sequences. Finally, the differentially expressed cDNAs were amplified by PCR and cloned into the pGEM-Teasy vector (Promega). cDNA clone sequence: Single bacterial colonies of the result of all the libraries were inoculated in 96-well microtiter plates containing LB and ampicillin (100 mg/L) and allowed to grow overnight with shaking at 37oC (18 hr at 320 RPM). The DNA was purified by standard alkaline lysis method with one modification at the end of the procedure, where the supernatant was passed through a multi-screen filter (Millipore) prior to DNA precipitation. The purified DNA was ressuspended in water. The sequencing reactions were performed with 200ng of DNA using standard protocols of the ThermoSequenase II dye terminator cycle sequencing kit (Amersham-Pharmacia Biotech) using universal M13 forward and reverse primers and the capillary sequencer MegaBACE 1000. The samples were electro injected by 2 KV for 100 seconds and the fragments were separated at 9 KV for 100 minutes. Sequence analysis of ESTs: The genome tool and facilities from EMBRAPA (www.genoma.embrapa.br) were used to test for sequence read quality by PHRED (Ewing et al 1998), sequences portion of poor quality were trimmed and low quality sequence (PHRED >20) removed from further analysis. Vector, primers and adaptors sequences were automatically trimmed from the high quality sequences. Only ESTs whose sequences exceed 250bp after trimming were considered for further analyses and submition.

text: Advanced secondary growth

text: E. coli

text: Storage root

text: Manihot esculenta

text: Storage root

text: MAGR

text: Plasmid


characteristic: INSDC last update

text: live

text: Generic

text: Most parenchyma

text: Mirassol

text: RNA extraction: Total RNA was extracted from cassava storage roots using phenol chloroform procedure. Construction of subtractive cDNA library: Polyadenylated RNA was purified from total RNA by using the mRNA Purification kit (Pharmacia). Double-stranded cDNAs were synthesized and subtractive PCR was conducted using the PCR-Select cDNA Subtraction Kit (Clontech). cDNAs were synthesized separately with 2ug of polyA+ RNA from the testers (Mirassol) and the driver (IAC 12.829) and separately digested with RsaI. The tester sample then was divided into two populations and each one ligated with adaptor 1 or 2R. Each tester population was hybridized separately with excess driver cDNA (33-fold). The two hybridization reactions were combined (with additional driver cDNA) for a second subtractive hybridization. The hybridizations led to equalization and enrichment of differentially expressed sequences. Finally, the differentially expressed cDNAs were amplified by PCR and cloned into the pGEM-Teasy vector (Promega). cDNA clone sequence: Single bacterial colonies of the result of all the libraries were inoculated in 96-well microtiter plates containing LB and ampicillin (100 mg/L) and allowed to grow overnight with shaking at 37oC (18 hr at 320 RPM). The DNA was purified by standard alkaline lysis method with one modification at the end of the procedure, where the supernatant was passed through a multi-screen filter (Millipore) prior to DNA precipitation. The purified DNA was ressuspended in water. The sequencing reactions were performed with 200ng of DNA using standard protocols of the ThermoSequenase II dye terminator cycle sequencing kit (Amersham-Pharmacia Biotech) using universal M13 forward and reverse primers and the capillary sequencer MegaBACE 1000. The samples were electro injected by 2 KV for 100 seconds and the fragments were separated at 9 KV for 100 minutes. Sequence analysis of ESTs: The genome tool and facilities from EMBRAPA (www.genoma.embrapa.br) were used to test for sequence read quality by PHRED (Ewing et al 1998), sequences portion of poor quality were trimmed and low quality sequence (PHRED >20) removed from further analysis. Vector, primers and adaptors sequences were automatically trimmed from the high quality sequences. Only ESTs whose sequences exceed 250bp after trimming were considered for further analyses and submition.

text: Advanced secondary growth

text: E. coli

text: Storage root

text: Manihot esculenta

text: Storage root

text: MALC

text: Plasmid


text: live

characteristic: INSDC status

text: Generic

text: immature

text: Ectocarpus siliculosus

text: sporophytes

text: immatures sporophytes normalised


text: live

text: Generic

characteristic: NCBI submission model

text: immature

text: Ectocarpus siliculosus

text: gametophytes

text: immatures gametophytes


text: live

text: Generic

text: mature

text: Ectocarpus siliculosus

text: sporophytes

text: matures sporophytes


text: live

text: Generic

text: Ectocarpus siliculosus

text: Ectocarpus siliculosus stress cloneminer


text: live

text: Generic

text: Ectocarpus siliculosus

text: Ectocarpus siliculosus stress


text: live

text: Generic

text: mature

text: Ectocarpus siliculosus

text: gametophytes

text: matures gametophytes


text: University of Edinburgh

text: live

text: Generic

text: library prepared from an infected E. huxleyi culture 12 hr post infection with EhV-86.

text: Emiliania huxleyi/EhV-86 mixed EST library

text: EhV-86-infected Emiliania huxleyi 12 hr post-infection

text: plasmid


text: University of Edinburgh

text: live

text: Generic

text: library prepared from an control E. huxleyi culture.

text: Emiliania huxleyi

text: Control Emiliania huxleyi (time zero)

text: plasmid


text: University of Edinburgh

text: live

text: Generic

text: library prepared from an infected E. huxleyi culture 24 hr post infection with EhV-86.

text: EhV-86-infected Emiliania huxleyi 24 hr post-infection

text: plasmid


text: University of Edinburgh

text: live

text: Generic

text: library prepared from an infected E. huxleyi culture 6 hr post infection with EhV-86.

text: EhV-86-infected Emiliania huxleyi 6 hr post-infection

text: plasmid


text: Central Institute of Freshwater Aquaculture

text: live

text: Generic

text: 1st strand cDNA was sythesized from total RNA of Rohu brain by SMART technique using power script reverse transcriptase of Clonetech and CDS-3M adapter of Evrogen. The cDNA was amplified by advantage-2 polymerase mix of Clonetech. The resulting ds-cDNA was normalized according to Trimmer-Direct cDNA normalization procedure of Evrogen. The cDNA were clonned in TOPO TA cloning vector of Invitrogen and sequenced in ABI-3130 Genetic Analyzers.

text: E.coli

text: Labeo rohita

text: brain

text: Rohu brain library

text: plasmid

text: TOPO TA (Invitrogen)


text: Universiti Kebangsaan Malaysia

text: live

text: Generic

text: Produced by Marine Functional Genomics Research Group. All the samples are taken from Kudat, Sabah, Malaysia

text: Eucheuma denticulatum

text: EcoRI

text: XhoI

text: Eucheuma denticulatum cDNA library

text: Phage

text: Uni-Zap (Stratagene)


text: The Plant Biotechnology Institute, National Research Council Canada

text: live

text: Generic

text: Topas (embryogenic line DH4079)

characteristic: cultivar

text: In vitro pollen development was induced in microspores isolated at the late-uninucleate to early-binucleate stage with a 18oC treatment for 3 days. The 3d in vitro microspores/pollen were collected by centrifugation.

text: 3d at 18oC

text: Brassica napus

text: Eco RI

text: Xho I

text: In vitro microspore/pollen

text: Plasmid

text: pBluescript SK+


text: live

text: Generic

characteristic: NCBI submission package

text: Topas (embryogenic line DH4079)

text: In vitro pollen development was induced in microspores isolated at the late-uninucleate to early-binucleate stage with a 18oC treatment for 5 days. The 5d in vitro microspores/pollen were collected by centrifugation.

text: 5d at 18oC

text: Brassica napus

text: Eco RI

text: Xho I

text: In vitro microspore/pollen

text: Plasmid

text: pBluescript SK+


text: live

text: Generic

text: Topas (embryogenic line DH4079)

text: In vitro pollen development was induced in microspores isolated at the late-uninucleate to early-binucleate stage with a 18oC treatment for 9 days. The 9d in vitro pollen were collected by centrifugation.

text: 9d at 18oC

text: Brassica napus

text: Eco RI

text: Xho I

text: In vitro pollen

text: Plasmid

text: pBluescript SK+


text: live

text: Generic

text: Topas (embryogenic line DH4079)

text: Pollen was collected from dehisced anthers on the day of anthesis.

text: Pollen

text: Brassica napus

text: Eco RI

text: Xho I

text: Pollen from dehisced anthers

text: Plasmid

text: pBluescript SK+


text: live

text: Generic

text: Topas (embryogenic line DH4079)

text: Ovules were dissected from ovaries of buds just before anthesis.

text: Mature ovules prior to fertilization

text: Brassica napus

text: Eco RI

text: Xho I

text: Unfertilized ovules

text: Plasmid

text: pBluescript SK+


text: College of Life Science, China West Normal University

text: live

text: Generic

text: mutation of three pistils (TP) of Triticum aestivum

text: Triticum aestivum

text: seedling leaf

text: Triticum aestivum TP seedling cDNA library


text: College of life Sciences, South-Central University for Nationalities

text: live

text: Generic

text: Lablab purpureus

text: purple epidermis

text: cDNA from purple epidermis of Dolichos purpureus stem whbd


text: Div. of Crop Protection, Central Research Institute for Jute and Allied Fibres

text: live

text: Generic

text: A SSH library of jute against 1 hr post challenge inoculation by Macrophomina phaseolina. Leaf samples of 21 days old CIM 036( a tolerant accession of jute against Macrophomina phaseolina infection) were inoculated with the mycelia culture of Macrophomina. After 1hr post inoculation, Total RNA were isolated from both the control and infected sample by the Trizol reagent (invitrogen). mRNA was isolated by mRNA isolation kit (Roche Applied Science). A differentially regulated subtracted cDNA pool were obtained from 1 hr. post infected leaf samples using the Clontech PCR select Subtraction Kit. This subtracted cDNA pool was then cloned in pJET vector and transformed in DH5-alpha cells and thus the different differentially regulated cDNA library was constructed.

text: Newly emerging leaves from 21 days old plants of jute(CIM036)

text: Corchorus capsularis

text: Leaves


text: live

text: Generic

text: A SSH library of jute against 6 hr post challenge inoculation by Macrophomina phaseolina. Leaf samples of 21 days old CIM 036( a tolerant accession of jute against Macrophomina phaseolina infection) were inoculated with the mycelia culture of Macrophomina. After 1hr post inoculation, Total RNA were isolated from both the control and infected sample by the Trizol reagent (invitrogen). mRNA was isolated by mRNA isolation kit (Roche Applied Science). The differentially regulated subtracted cDNA pool were obtained from 6 hr. post infected leaf samples using the Clontech PCR select Subtraction Kit. This subtracted cDNA pool were then cloned in pJET vector and transformed in DH5-alpha cells and thus the different differentially regulated cDNA library was constructed.

text: Corchorus capsularis

text: Leaves


text: Iwate Biotechnology Research Center

text: live

text: Generic

text: The mRNA was prepared from fruiting body after harvesting.

text: Lentinula edodes

text: gill of fruiting body

text: Lentinula edodes PCR-select forward subtraction library


text: Iwate Biotechnology Research Center

text: live

text: Generic

text: The mRNA was prepared from fresh fruiting body.

text: Lentinula edodes

text: gill of fruiting body

text: Lentinula edodes PCR-select reverse subtraction library


text: live

text: Generic

text: Housui

text: Pyrus pyrifolia

text: leaf

text: National Institute of Fruit Tree Science_NLE2


text: live

text: Generic

text: Housui

text: Pyrus pyrifolia

text: flower

text: National Institute of Fruit Tree Science_NFW2


text: live

text: Generic

text: Housui

text: Pyrus pyrifolia

text: fruit

text: National Institute of Fruit Tree Science_NFR1


text: live

text: Generic

text: Housui

text: Pyrus pyrifolia

text: fruit

text: National Institute of Fruit Tree Science_NFR2


text: live

text: Generic

text: Housui

text: Pyrus pyrifolia

text: fruit

text: National Institute of Fruit Tree Science_NFR5


text: International Crops Research Institute for the Semi-Arid Tropics (ICRISAT)

text: live

text: Generic

text: cDNA library was constructed from root tissues of 6 days after inoculated (DAI) pigeonpea seedlings of ICPL20102 genotype imposed to Fusarium wilt stress in greenhouse conditions. The cDNA fragments were cloned into pGEM-T Easy vector and subsequently sequenced by Sanger sequencing approach using M13 primers.

text: E. coli (DH5a)

characteristic: lab-host

text: Cajanus cajan

text: EcoRI

text: root

text: Fusarium wilt challenged (6 DAI) pigeonpea genotype ICPL20102 root cDNA library

text: plasmid

text: pGEM-T Easy vector


text: live

text: Generic

text: cDNA library was constructed from root tissues of 10 days after inoculated (DAI) pigeonpea seedlings of ICPL20102 genotype imposed to Fusarium wilt stress in greenhouse conditions. The cDNA fragments were cloned into pGEM-T Easy vector and subsequently sequenced by Sanger sequencing approach using M13 primers.

text: E. coli (DH5a)

text: Cajanus cajan

characteristic: organism

text: EcoRI

text: root

text: Fusarium wilt challenged (10 DAI) pigeonpea genotype ICPL20102 root cDNA library

text: plasmid

text: pGEM-T Easy vector


text: live

text: Generic

text: cDNA library was constructed from root tissues of 15 days after inoculated (DAI) pigeonpea seedlings of ICPL20102 genotype imposed to Fusarium wilt stress in greenhouse conditions. The cDNA fragments were cloned into pGEM-T Easy vector and subsequently sequenced by Sanger sequencing approach using M13 primers.

characteristic: description

text: E. coli (DH5a)

text: Cajanus cajan

text: EcoRI

text: root

text: Fusarium wilt challenged (15 DAI) pigeonpea genotype ICPL20102 root cDNA library

text: plasmid

text: pGEM-T Easy vector


text: live

text: Generic

text: cDNA library was constructed from root tissues of 20 days after inoculated (DAI) pigeonpea seedlings of ICPL20102 genotype imposed to Fusarium wilt stress in greenhouse conditions. The cDNA fragments were cloned into pGEM-T Easy vector and subsequently sequenced by Sanger sequencing approach using M13 primers.

text: E. coli (DH5a)

text: Cajanus cajan

text: EcoRI

text: root

text: Fusarium wilt challenged (20 DAI) pigeonpea genotype ICPL20102 root cDNA library

characteristic: title

text: plasmid

text: pGEM-T Easy vector