characteristic: External Id

text: Washington University School of Medicine

text: live

text: Generic

text: GoldRush

text: Total RNA was extracted separately from each stage [dormant shoot internodes, active shoot internodes, actively-growing shoot internodes], using the 'pine tree' method. Poly(A)+mRNA was isolated twice from total RNA from each stage using the Oligotex Direct mRNA kit (Qiagen). mRNA was reverse transcribed into double stranded cDNA using a modified oligo18(dT) primer with an identifying tag sequence (see table below). cDNA's from different stages were pooled in equal amounts before adaptor ligation. Tag identification when sequencing from 5' end: Stage 1 (dormant shoot internodes) insert 18(A)TCGTG; Stage 2 (active shoot internodes) insert 18(A)TGCTG; Stage 3 (actively-growing shoot internodes) insert 18(A)TCGGT; Tag identification when sequencing from 3' end: Stage 1 (dormant shoot internodes) CACGA18(T) insert; Stage 2 (active shoot internodes) CAGCA18(T) insert; Stage 3 (actively-growing shoot internodes) ACCGA18(T) insert; Double stranded cDNAs were size selected (more than 450 bp), adaptored with EcoRI adapters at both ends and then digested with NotI. The cDNAs were then directionally cloned into EcoR1-NotI digested pBS II SK(+) phagemid vector(Stratagene). Identification of adaptors and tags in 5'-end sequenced clones: (Vector)...TAAGCTT(End Vector)(Start EcoR1_adaptor)GATATCGAATTCCATTGTGTTGGG (End EcoR1_adaptor)(Start Insert)....AAAAAAAAAAAAAAAAAA(End Insert) (Start Tag)TGCGA(End Tag)(Start NotI_site/Vector)GCGGCCGCCACCGCGG... The total number of white colony forming units (cfu) in the primary library before amplification was 2.7x10^7 cfu (colony forming units). The background of empty clones was less than 2%. Inserts ranged from 0.5kb to 4 kb, as determined by PCR. Purified plasmid DNA from the primary library was converted to single-stranded circles and used as a template for PCR amplification using the T7 and T3 priming sites flanking the cloned cDNA inserts. The purified PCR products, representing the entire cloned cDNA population, were used as a driver for normalization. Hybridization between the single-stranded library and the PCR products was carried out for 44 hours at 30C. Unhybridized single-stranded DNA circles were separated from hybridized DNA rendered partially double-stranded and electroporated into DH10B cells to generate the normalized library. The total number of clones with insert was 8x10^5 cfu. Background of empty clones was less than 1%.

characteristic: description

text: DH10B ampicillin resistant

text: Malus domestica

text: NotI

text: Shoot internodes

text: Mdst

text: Phagemid (ampicillin resistant)

text: pBluescript II SK (+)


status: PUBLIC

text: Medical University of South Carolina

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: hepatopancreas

tag: attribute

characteristic: cell type

text: Crassostrea virginica

characteristic: organism

text: Sfi I

text: Sfi I

text: Cv Hepatopancreas

characteristic: title

text: Plasmid

characteristic: vector


text: Medical University of South Carolina

text: live

text: Generic

text: Hemocyte

text: Crassostrea virginica

text: Cv Hemo Cadmium Rev SSH

text: Plasmid


text: Medical University of South Carolina

text: live

text: Generic

text: Hemocyte

text: Crassostrea virginica

text: Cv Hemo Hypoxia Forw SSH

text: Plasmid


text: Medical University of South Carolina

text: live

text: Generic

text: Hemocyte

text: Crassostrea virginica

text: Cv Hemo Hypoxia Rev SSH

text: Plasmid


text: Medical University of South Carolina

text: live

text: Generic

text: Hemocyte

text: Crassostrea virginica

text: Cv Hemo 4hr Immune Forward SSH

text: Plasmid


text: Medical University of South Carolina

text: live

text: Generic

text: hepatopancreas

text: Crassostrea virginica

text: Cv Hepato 4hr Immune Forward SSH

text: Plasmid


text: Medical University of South Carolina

text: live

text: Generic

text: hepatopancreas

text: Crassostrea virginica

text: Cv Hepato 24hr Immune Forward SSH

text: Plasmid


text: Medical University of South Carolina

text: live

text: Generic

text: hepatopancreas

text: Crassostrea virginica

text: Cv Hepato Cadmium Forward SSH

text: Plasmid


text: Medical University of South Carolina

text: live

text: Generic

text: Hemocyte

text: Crassostrea virginica

text: Cv Hemo 4hr Immune Reverse SSH

text: Plasmid


text: Medical University of South Carolina

text: live

text: Generic

text: hepatopancreas

text: Crassostrea virginica

text: Cv Hepato Hypoxia Forward SSH

text: Plasmid


text: Medical University of South Carolina

text: live

text: Generic

text: Hemocyte

text: Crassostrea virginica

text: Sfi I

text: Sfi I

text: Hemocyte Immune Challenged unmodified cDNA SMART Library

text: Plasmid


text: University of Missouri-Columbia

text: live

text: Generic

text: Funding: A grant from the Monsanto Company to the University of Missouri. Genetic Source: Day 6 pig blastocyst stage embryos were flushed from a gilt's reproductive tract in TL Hepes and cultured in NCSU23 (1) for 3 hours prior to vitrification. For vitrification, embryos were cultured in 1M dimethylsulfoxide and 1.3M ethylene glycol in Tissue Culture Medium (TCM) and 20% newborn calf serum for 5 minutes at 39 degrees Celsius. Then embryos were transferred to 3.2M ethylene glycol and 0.6M sucrose in TCM and 20% newborn calf serum for 45 to 60 seconds at 39 degrees Celsius. Capillary action was used to load the embryos in open pulled straws and the straws were plunged into liquid nitrogen. At thawing embryos were placed in 0.2M sucrose in TCM and 20% newborn calf serum for 1 minute, then 01.M sucrose in TCM and 20% newborn calf serum for 5 minutes. Embryos were then rinsed and cultured in NCSU23 for 12 or 24 hours. Library Construction (PCR Protocol): The amount of mRNA that was recovered from embryos was quite limiting and was not sufficient for library production with a standard protocol. Therefore, PCR-based protocol was utilized for producing libraries. The blastocysts were then rinsed with 50 ul polyvinylalcohol-PBS in depc treated medium three times and snap frozen with 1 ul super-RNAsin in a 0.5ml Rnase-free tube. Hybond messenger affinity paper was used to isolated the mRNA (2). The mRNA was reverse transcribed with a NotI-tag-dT18 oligonucleotide and a SMART oligonucleotide (Clontech) modified to contain a SalI site to generate full-length cDNA with a sequence complementary to the SMART oligonucleotide. Sequences within the SMART and dT oligonucleotides were used as primers to amplify the cDNAs by PCR with pfu turbo polymerase (Stratagene). The resulting PCR products were purified, digested with NotI and SalI and size fractionated by using a Chroma Spin-400 followed by a Chroma Spin-1000 column (Clontech). Purified cDNA from each PCR reaction was ligated into the pCMV-SPORT6 vector. Preliminary Library Characterization: Randomly chosen clones from each library were analyzed by restriction digestion to determine average insert size (96 clones) and by sequencing (~3 96-well plates) to confirm library quality [e.g. the presence of short polyA+ tails, genomic DNA contamination (must be <1%), ribosomal RNA clones (must be <1%), etc.] and to provide a sequence database representing the predominant clones in each library. The clones were sequenced at the University of Missouri-Columbia DNA Core Facility. Bioinformatics work was performed by GK Springer's bioinformatics group (WG Spollen, J Ries, A Gullin, AA Khambati, RV Patel, CM Topinka, SB Bhuiyan) in Computer Science and Health Management and Informatics Departments at the University of Missouri-Columbia. Clone Requests: Requests for clones should be made to the Director of the University of Missouri DNA Core facility at: porcine@rnet.missouri.edu. Citations: 1. Im G-S, Lai L, Liu Z, Hao Y and Prather RS. In vitro development of preimplantation porcine nuclear transfer embryos cultured in different media and gas atmospheres. Theriogenology 61: 1125-1135, 2004. 2. Anderson JE, Matteri RL, Abeydeera LR, Day BN and Prather RS. Degradation of maternal Cdc25c during the maternal to zygotic transition is dependent upon embryonic transcription. Molecular Reproduction & Development 60: 181-188, 2001.

text: Blastocyst after cryopreservation

characteristic: dev-stage

text: Sus scrofa

text: pcrybst

text: GTGTAACCTA


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: Homo sapiens

text: Homo sapiens library (Ebert L)


text: National Cancer Institute / NIH

text: live

text: Generic

text: Tissue was collected from two pooled placentas from the 21st day of pregnancy. 1st strand cDNA was primed with a Not I - oligo(dT) primer, double-stranded cDNA was cloned into the Not I and EcoRV sites of pExpress-1. Library was size-selected for >1.25 kb fragments for an average insert size of 2.05 kb. A normalized version of this library is also available (NIH_MGC_270). Library was constructed by Open Biosystems (Huntsville, AL). Note: this is a Mammalian Gene Collection library

text: DH10B TonA

characteristic: lab-host

text: placenta

characteristic: organ

text: Rattus norvegicus

text: EcoRV

text: NotI

text: whole placenta, 2 pooled

characteristic: tissue-type

text: plasmid


text: National Cancer Institute / NIH

text: live

text: Generic

text: Tissue was collected from two pooled placentas from the 21st day of pregnancy. 1st strand cDNA was primed with a Not I - oligo(dT) primer, double-stranded cDNA was cloned into the Not I and EcoRV sites of pExpress-1. Library was size-selected for >1.25 kb fragments for an average insert size of 2.15 kb. Library was normalized to Cot7. A non-normalized version of this library is also available (NIH_MGC_269). Library was constructed by Open Biosystems (Huntsville, AL). Note: this is a Mammalian Gene Collection library

text: DH10B TonA

text: placenta

text: Rattus norvegicus

text: EcoRV

text: NotI

text: whole placenta, 2 pooled

text: plasmid


text: Kyoto University

text: live

text: Generic

text: Ciona savignyi

text: Yutaka Satou beta-catenin downstream gene enriched cDNA library


text: Kyoto University

text: live

text: Generic

text: cleavage stage

text: Ciona savignyi

text: Yutaka Satou unpublished cDNA library (cscl)


text: Kyoto University

text: live

text: Generic

text: gastrula stage

text: Ciona savignyi

text: Yutaka Satou unpublished cDNA library (csga)


text: Kyoto University

text: live

text: Generic

text: larva

text: Ciona savignyi

text: Yutaka Satou unpublished cDNA library (cslv)


text: Kyoto University

text: live

text: Generic

text: mature adult

text: Ciona savignyi

text: Yutaka Satou unpublished cDNA library (csma)


text: Kyoto University

text: live

text: Generic

text: tailbud stage

text: Ciona savignyi

text: Yutaka Satou unpublished cDNA library (cstb)


text: Kyoto University

text: live

text: Generic

text: egg

text: Ciona savignyi

text: Yutaka Satou unpublished cDNA library (csef1)


text: Kyoto University

text: live

text: Generic

text: egg

text: Ciona savignyi

text: Yutaka Satou unpublished cDNA library (csef2)


text: live

text: Generic

text: GoldRush

characteristic: cultivar

text: Total RNA was extracted from freeze dried leaf tissue Stage I, using a standard Phenol chloroform extraction method. Poly(A)+mRNA was isolated from total RNA using the PolyATtract mRNA Isolation system III (Promega). The library was prepared using the Invitrogen Life Technologies, Superscript Plasmid System with gateway technology for cDNA Synthesis and cloning. Complementary DNA was synthesized from mRNA using an anchored Poly (dT) sequence with a NotI restriction site. Sal I linker adapters were ligated to the blunt ended cDNA fragments followed by restriction with NotI. The cDNA fragments were directionally cloned into the NotI-SalI restriction site of the pSPORT 1 vector. The ligated cDNA fragments were transformed into E. coli ElectroMax Dh10B host cells. Transformation efficiency: 8.5E+07 Average insert Size by PCR: 1100 bp

text: DH10B ampicillin resistant

text: Malus domestica

text: NotI

text: Sal I

text: Leaf

text: Mdlv

text: Phagemid (ampicillin resistant)

text: pSPORT 1


characteristic: External Id

text: DFCI

text: live

text: Generic

text: all cells

text: all stages

text: Caenorhabditis elegans

text: male and hermaphrodite

characteristic: sex

characteristic: strain

text: all tissues

text: pDONR201 Entry vector


text: Chung-Ang University

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: Eisenia andrei

text: EcoRI

text: XhoI

text: midgut

text: Eisenia andrei midgut cDNA library

text: pCMV-Script XR


text: Graduate School of Bioagricultural Sciences, Nagoya University

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Haruna-nijyo

text: Hordeum vulgare subsp. vulgare

text: root

text: 6 days salt stressed cDNA library, cv. Haruna-nijyo root


text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Hordeum vulgare

text: leaf

text: 6 days salt stressed cDNA library, leaf


text: live

text: Generic

text: Hordeum vulgare

characteristic: organism

text: root

text: 6 days salt stressed cDNA library, root


text: IBP-CNRS, University of Paris XI

text: live

text: Generic

text: Tamara

text: RT-PCR were performed on shoot apex RNA. Resulting amplification product was cloned in a pGEM-T easy vector (Promega) and SP6/T7 sequenced.

characteristic: description

text: 3-5 leaves plantlets

text: shoot apex

text: Sorghum bicolor

text: hermaphrodite

text: shoot apex

text: PCR amplification from tel cDNA (sorghum)

characteristic: title

text: pGEM-T easy vector (Promega)


text: IBP-CNRS, University of Paris XI

text: live

text: Generic

text: 3-5 leaves plantlets

characteristic: dev-stage

text: shoot apex

text: Digitaria sanguinalis

text: hermaphrodite

text: shoot apex

text: PCR amplification from tel cDNA (hairy crabgrass)

text: pGEM-T easy vector (Promega)

characteristic: vector


text: IBP-CNRS, University of Paris XI

text: live

text: Generic

text: 3-5 leaves plantlets

text: shoot apex

characteristic: organ

text: Secale cereale

text: hermaphrodite

text: shoot apex

text: PCR amplification from tel cDNA (rye)

text: pGEM-T easy vector (Promega)


text: Scottish Crop Research Institute

text: live

text: Generic

text: mRNA extracted from mixed stage Xiphinema index cloned directionally into pSPORT plasmid vector.

text: mixed

text: Xiphinema index

text: SalI

text: NotI

text: Xiphinema index whole nematode pSPORT

text: plasmid

text: pSPORT


text: Scottish Crop Research Institute

text: live

text: Generic

text: mRNA extracted from mixed stage Xiphinema index cloned directionally into pBluescript plasmid vector.

text: mixed

text: Xiphinema index

text: NotI

text: EcoRI

text: Xiphinema index whole nematode pBS

text: plasmid

text: pBluescript


text: Scottish Crop Research Institute

text: live

text: Generic

text: mRNA extracted from mixed stage Xiphinema index cloned directionally into pDNR LIB plasmid vector.

text: mixed

text: Xiphinema index

text: EcoRI

text: XhoI

text: Xiphinema index whole nematode pDNR

text: plasmid

text: pDNR LIB


text: Scottish Crop Research Institute

text: live

text: Generic

text: Total RNA extracted from basal bulbs dissected from mixed stage Xiphinema index cloned directionally into pDNR LIB plasmid vector.

text: mixed

text: Xiphinema index

text: EcoRI

text: XhoI

text: Xiphinema index basal bulb

text: plasmid

text: pDNR LIB


text: Washington University School of Medicine

text: live

text: Generic

text: The library was constructed by Irina Ronko and Dr. Makedonka Mitreva (mmitreva@watson.wustl.edu) at Washington University, St. Louis. The cDNA was made by using Dynabead oligo-dT priming (Dynal). PCR based library using a modified protocolfrom the SMART PCR cDNA Synthesis Kit from Clontech. Directionally cloned into the UDG sites of pAMP1. Radopholus similis total RNA was provided by Dr. Godelieve Gheysen of Ghent University (lighe@gengenp.rug.ac.be).

text: Mixed

characteristic: lab-host

text: Radopholus similis

text: 5': SalI

text: 3': NotI

text: Radopholus similis pAMP1 v1

text: plasmid (ampicillin resistant)

text: pAMP1 (gibco)


text: Genoscope - Centre National de Sequencage

text: live

text: Generic

text: Pietu G., Cros N., Leger J.J., Dechesne C.A. Substracted library from atrophied and control soleus muscles Substraction was performed according to Diatchenko et al. (Diatchenko L, Lau YF, Campbell AP, Chenchik A, Moqadam F, Huang B, Lukyanov S, Lukyanov K, Gurskaya N, Sverdlov ED, Siebert PD. Suppression subtractive hybridization: a method for generating differentially regulated or tissue-specific cDNA probes and libraries. Proc Natl Acad Sci U S A. 1996; 93 :6025-30) Rats were female Sprague Dawley between 200 and 220g. Soleus muscle atrophy was performed by 14 days of hindlimb suspension.

text: Rattus norvegicus

text: Female

text: Sprague-Dawley

text: muscle

characteristic: tissue-type

text: Rattus norvegicus muscle Sprague-Dawley

text: pCRII-TOPO


characteristic: External Id

text: live

text: Generic

text: Pietu G., Cros N., Leger J.J., Dechesne C.A. Mus musculus substracted libraries, detail of cDNA collection: pool of 4 cDNA libraries: C57BL/10mdx diaphragm cDNA subtracted from C57BL/10ScSn diaphragm cDNA, C57BL/10ScSn diaphragm cDNA subtracted from C57BL/10mdx diaphragm cDNA, C57BL/10mdx hindlimb muscle cDNA substracted from C57BL/10ScSn hindlimb muscle cDNA, C57BL/10ScSn hindlimb muscle cDNA substracted from C57BL/10mdx hindlimb muscle cDNA.

text: Mus musculus

text: male

text: muscle

text: mdx substracted from control

text: pCRII-TOPO


text: live

text: Generic

text: Mus musculus

text: male

characteristic: sex

text: muscle

text: control substracted from mdx

text: pCRII-TOPO


text: Central Institute of Medicinal and Aromatic Plants

text: live

text: Generic

text: Suppression subtractive hybridization was carried out using the cDNA populations derived from the callus (Tester) and trichome (Driver) tissues of Mentha arvensis L. The callus specific messages were cloned on the pUC18.

text: invitro maintained callus subcultured every 20 days

text: E.coli DH5 strain

text: shoot

text: Mentha arvensis

text: SmaI

text: SmaI

text: Hermaphrodite

text: Callus

text: Mentha arvensis L. callus specific subtracted cDNA library

text: Plasmid


text: live

text: Generic

text: secretory cell

characteristic: cell type

text: Suppression subtractive hybridization was carried out using the cDNA populations derived from the trichome (Tester) and callus (Driver) tissues of Mentha arvensis. The trichome specific messages were cloned on the pUC18.

text: 85 days after planting

text: E. coli DH5 strain

text: Leaf

text: Mentha arvensis

text: SmaI

text: SmaI

text: Hermaphrodite

text: peltate glandular trichome

text: Mentha arvensis L. trichome specific subtracted cDNA library

text: Plasmid


text: Graduate School of Biotechnology, Korea University

characteristic: INSDC center name

text: live

text: Generic

text: After the modified subtractive hybridization based on MOS (Nucleic Acids Research, Vol 28, No 20, e90), cDNA fragments were cloned by TA cloning method

text: insect infestation & vegetative stage

text: Oryza minuta

characteristic: strain

text: leaf

text: Oryza minuta subtracted cDNA library insect infestation

text: plasmid


text: Roslin Institute

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: bovine monocyte

text: Normalised library constructed from pooled monocytes from Bos taurus (Holstein) and Bos indicus (Sahiwals) cattle subjected to various stimuli, including infection with the protozoan parasite Theileria annulata

text: Bos sp.

text: EcoRV(lost)

text: NotI

text: blood

text: pBluescriptII(SK+)


text: Roslin Institute

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Theileria annulata sequence identified within a normalised library constructed from pooled monocytes from Bos taurus and Bos indicus cattle subjected to various stimuli, including infection with the protozoan parasite Theileria annulata

text: macroschizont, trophozoite & sporozoite

text: Theileria annulata

text: EcoRV(lost)

text: NotI

text: pBluescriptII(SK+)


text: Nihon University College of Bioresource Sciences Laboratory of Enzymology and Molecular Biology

text: live

text: Generic

characteristic: NCBI submission package

text: 2d embryo

text: Gallus gallus

characteristic: organism

text: female

text: Gallus gallus 2d embryo

text: pCR 4Blunt-TOPO


text: UAS, GKVK, Bangalore-560065

text: live

text: Generic

text: Using tomato EST CER1 specific primers amplified from cDNA of Nicotiana benthamiana and cloned into TA (pTZ57R/T) subsequently subcloned into pYL156 virus (VIGS) vector. Using syringe infiltrated into host Nicotiana benthamiana plant as well as tomato

characteristic: description

text: Leaf

text: Nicotiana benthamiana

text: Hermaphrodite

text: Leaf tissue

text: cDNA library of Nicotiana benthamiana

characteristic: title

text: pYL156 virus (VIGS)


text: Inpt - Ensat, Pole de Biotechnologie Vegetale

text: live

text: Generic

text: Helianthus annuus globular embryo; inbred line: Ha300b

text: globular embryo

characteristic: dev-stage

text: Helianthus annuus

text: HaGlbR


text: live

text: Generic

text: Helianthus annuus heart-shaped embryo; inbred line: Ha300b

text: heart-shaped embryo

text: Helianthus annuus

text: HaHeaR


text: live

text: Generic

text: Helianthus annuus cotyledonary embryo; inbred line: Ha300b

text: cotyledonary embryo

text: Helianthus annuus

text: HaCotR


text: live

text: Generic

text: Helianthus annuus heart-shaped embryo vs cotyledonary embryo: inbred line: Ha300b

text: Helianthus annuus

text: HaHeaS


text: c/o UniMi DiProVe, Parco Tecnologico Padano

text: live

text: Generic

text: post-climacteric

text: Prunus persica

text: fruit mesocarp


text: live

text: Generic

text: from pre-climacteric to climacteric

text: Prunus persica

text: fruit mesocarp


text: The Natural History Museum

text: live

text: Generic

text: Julodis onopordi

text: Whole

text: Julodis onopordi cDNA

text: pCRII

characteristic: vector


text: The Natural History Museum

text: live

text: Generic

text: Eucinetus sp. APV-2005

text: Whole

text: Eucinetus sp. cDNA

text: pCRII


text: The Natural History Museum

text: live

text: Generic

text: Dascillus cervinus

text: Whole

text: Dascillus cervinus cDNA

text: pCRII


text: The Natural History Museum

text: live

text: Generic

text: Mycetophagus quadripustulatus

text: Whole

text: Mycetophagus quadripustulatus cDNA

text: pCRII


text: The Natural History Museum

text: live

text: Generic

text: Tribolium confusum

text: Whole

text: Tribolium confusum cDNA

text: pCRII


text: The Natural History Museum

text: live

text: Generic

text: Micromalthus debilis

text: Whole

text: Micromalthus debilis cDNA

text: pCRII


text: The Natural History Museum

text: live

text: Generic

text: Sphaerius sp. APV-2005

text: Whole

text: Sphaerius sp. cDNA

text: pCRII


text: Universidad de Murcia

text: live

text: Generic

text: Cephalota litorea

text: Male

text: Testis

text: Cicindela litorea cDNA

text: pCRII


text: Universidad de Murcia

text: live

text: Generic

text: Calomera littoralis

text: Male

text: Testis

text: Cicindela littoralis cDNA

text: pCRII


text: The Natural History Museum

text: live

text: Generic

text: Georissus sp. APV-2005

text: Whole

text: Georissus sp. cDNA

text: pCRII


text: The Natural History Museum

text: live

text: Generic

text: Hister sp. APV-2005

text: Whole

text: Hister sp. cDNA

text: pCRII


text: The Natural History Museum

text: live

text: Generic

text: Scarabaeus laticollis

text: Whole

text: Scarabaeus laticollis cDNA

text: pCRII


text: The Natural History Museum

text: live

text: Generic

text: Agriotes lineatus

text: Whole

text: Agriotes lineatus cDNA

text: pCRII


text: University of North Carolina Wilmington

text: live

text: Generic

text: Total RNA samples were prepared individually from each tissue, checked for quality, and then pooled for construction and normalization of a cDNA library by Invitrogen. Plasmids were isolated and inserts sequenced from their 5'-ends by the Blue Crab Molecular Genetics Laboratory at the University of North Carolina Wilmington. Traces were trimmed, compared (BLASTx) to NCBI non-redundant protein database as of 19 July 2004, and processed for submission to dbEST by trace2dbEST software (Parkinson, Anthony and Blaxter, unpublished software).

text: Adult

text: Callinectes sapidus

text: Pooled hypodermal epithelium from the mid-dorsal region and arthrodial membrane of premolt (stage D2) and 3-hour postmolt crabs

text: Blue crab hypodermis, normalized

text: pCMV Sport 6.1


text: The Natural History Museum

text: live

text: Generic

text: Euclidia glyphica

text: Whole

text: Euclidia glyphica cDNA

text: pCRII


text: The Natural History Museum

text: live

text: Generic

text: Larvae

text: Papilio dardanus

text: Imaginal Wing Disc

text: Papilio dardanus cDNA

text: pCRII


text: The Natural History Museum

text: live

text: Generic

text: Gryllus bimaculatus

text: Whole

text: Gryllus bimaculatus cDNA

text: pCRII


text: The Natural History Museum

text: live

text: Generic

text: Sphodromantis centralis

text: Whole

text: Sphodromantis centralis cDNA

text: pCRII


text: The Natural History Museum

text: live

text: Generic

text: Aleurothrixus sp. APV-2005

text: Whole

text: Aleurothrixus sp. cDNA

text: pCRII


text: The Natural History Museum

text: live

text: Generic

text: Phaeostigma major

text: Whole

text: Phaeostigma major cDNA

text: pCRII


text: The Natural History Museum

text: live

text: Generic

text: Limnephilus flavicornis

text: Whole

text: Limnephilus flavicornis cDNA

text: pCRII


text: The Natural History Museum

text: live

text: Generic

text: Panorpa cf. vulgaris APV-2005

text: Whole

text: Panorpa vulgaris cDNA

text: pCRII


text: Universitat de Barcelona

text: live

text: Generic

text: Dysdera erythrina

text: Whole

text: Dysdera erythrina cDNA

text: pCRII


text: The Natural History Museum

text: live

text: Generic

text: Collected in leaf litter, Whitley Wood, New Forest, Hampshire, UK

text: Immature

text: Julida sp. APV-2005

text: Whole

text: Julida sp. cDNA

text: pCRII


text: The Chinese University of Hong Kong

text: live

text: Generic

text: XLOLR

characteristic: lab-host

text: Ovary

characteristic: organ

text: Metapenaeus ensis

text: EcoRI

text: XhoI

text: Ovary

text: ZAP Express cDNA library of Metapenaeus ensis

text: phagemid

text: pBK-CMV


characteristic: External Id

text: Queens University of Belfast, Grosvenor Road, Belfast BT12 6BJ, UK

text: live

text: Generic

text: Breast cancer cell line

characteristic: cell-line

text: Subtracted cDNA Library

text: Rattus norvegicus

text: Breast cancer cell line


text: live

text: Generic

text: Breast cancer invasive cell line

text: Subtracted cDNA Library

text: Rattus norvegicus

text: Breast cancer invasive cell line


text: University of Bath

text: live

text: Generic

characteristic: cultivar

text: Manihot esculenta

text: Leaf

characteristic: tissue-type

text: cDNA-AFLP TDFs from cassava undergoing the hypersensitive response induced bt Pseudomonas syringae pv. tomato DC3000


text: University of Insubria

characteristic: INSDC center name

text: live

text: Generic

text: Dicentrarchus labrax

text: adult liver

text: D. labrax liver cDNA library


text: Cardiff University

characteristic: INSDC first public

text: live

text: Generic

text: The library was prepared using protocols given by the supplier (Clontech).

text: Adult

text: Lumbricus rubellus

text: mixed

characteristic: sex

text: Reproductive Tissue Areas - Segment Numbers 10 to 30

text: Earthworm Reproductive Subtraction library 1

text: pGEM-T


text: Washington University School of Medicine

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: GoldRush

text: Total RNA was extracted from freeze dried leaf tissue Stage III, using the method described by Wang and Vodkin (Plant Molecular Biology Reporter 12:132-145, 1994). Poly(A)+ mRNA was isolated from total RNA using the PolyATtract mRNA Isolation system III (Promega). The library was prepared using the Stratagene pBluescript II XR cDNA library construction kit. Complementary DNA was synthesized from mRNA using a Poly (dT) sequence with a XhoI restriction site. EcoRI adapters were ligated to the blunt ended cDNA fragments followed by restriction with XhoI. The cDNA insert is protected from XhoI digestion via methylation during first strand cDNA synthesis. The cDNA fragments were directionally cloned into the EcoRI-XhoI restriction site of the pBluescript vector. The ligated cDNA fragments were transformed into E. coli ElectroMax DH10B host cells. Transformation efficiency: 1.2E+07 Average insert Size by PCR: 1205 bp

text: DH10B ampicillin resistant

text: Malus domestica

text: Xho I

text: Eco RI

text: Leaf

text: Phagemid (ampicillin resistant)

text: pBluescript II SK (+)


text: Penn State University

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: This is a directionally cloned, non-normalized library. The library was constructed from equal amounts of mRNA isolated from an E. californica cell suspension culture 0.5, 6 and 12 hours after elicitation with 5 ug/ml of yeast elicitor. This library has been generated by the Floral Genome Project (FGP). The Floral Genome Project is funded by NSF's Plant Genome Research Program (DBI-0115684). More information about the project can be obtained at http://fgp.bio.psu.edu

text: XL1 Blue MRF

text: Eschscholzia californica

text: cell culture suspension


text: Penn State University

text: live

text: Generic

text: This is a directionally cloned, non-normalized library. This library has been generated by the Floral Genome Project (FGP). The Floral Genome Project is funded by NSF's Plant Genome Research Program (DBI-0115684). More information about the project can be obtained at http://fgp.bio.psu.edu

characteristic: description

text: <2.5mm buds

text: SOLR

text: Saruma henryi

characteristic: organism

text: EcoRI

text: flower buds

text: Phagemid

text: pBluescript SK (+/-)


text: Penn State University

text: live

text: Generic

text: This is a directionally cloned, non-normalized library. Created with the Clontech SMART cDNA library construction kit, electroporation. 25ng 50uls plated 2,000 colonies This library has been generated by the Floral Genome Project (FGP). The Floral Genome Project is funded by NSF's Plant Genome Research Program (DBI-0115684). More information about the project can be obtained at http://fgp.bio.psu.edu

text: Tunderbolt (TOP 10)

text: Illicium parviflorum

text: Sfi A

text: Sfi B

text: flower buds

characteristic: title


text: US Department of Agriculture (USDA), ARS, PSI

text: live

text: Generic

text: SOLR

text: Vaccinium corymbosum

text: EcoRI

text: flower buds with budscales

text: Phagemid

text: pBluescript SK (+/-)

characteristic: vector


text: Westfalische Wilhelms Universitat Munster

text: live

text: Generic

text: cDNA from mock-inoculated leaves was subtracted from cDNA from infected leaves, resulting in mostly fungal cDNA.

text: infected wheat leaves, 5, 9 and 12 dpi

characteristic: dev-stage

text: race 32

characteristic: isolate

text: Puccinia graminis f. sp. tritici

text: SSH-library of Puccinia graminis infected wheat leaves.


characteristic: External Id

text: Institute of Medical Science, University of Tokyo

text: live

text: Generic

text: Homo sapiens

text: brain

text: Sugano cDNA library, brain


text: live

text: Generic

text: Homo sapiens

text: adrenal gland

text: Sugano cDNA library, adrenal gland


text: live

text: Generic

text: Homo sapiens

text: amygdala

text: Sugano cDNA library, amygdala


text: live

text: Generic

text: smooth muscle cell

characteristic: cell type

text: Homo sapiens

text: coronary artery

text: Sugano cDNA library, coronary artery smooth muscle cell


text: live

text: Generic

text: Homo sapiens

text: cerebellum

text: Sugano cDNA library, cerebellum


text: live

text: Generic

text: endothelial cell

text: Homo sapiens

text: coronary artery

text: Sugano cDNA library, coronary artery endothelial cell


text: live

text: Generic

text: Homo sapiens

text: cerebrum

text: Sugano cDNA library, cerebrum


text: live

text: Generic

text: Homo sapiens

text: corpus callosum

text: Sugano cDNA library, corpus callosum


text: live

text: Generic

text: Homo sapiens

text: caudate nucleus

text: Sugano cDNA library, caudate nucleus


text: live

text: Generic

text: Homo sapiens

text: colon

text: Sugano cDNA library, colon


text: live

text: Generic

characteristic: cell-line

text: melanoma

text: Homo sapiens

text: Sugano cDNA library, COL0679


text: live

text: Generic

text: Homo sapiens

text: colon mucosa

characteristic: tissue-type

text: Sugano cDNA library, colon mucosa


characteristic: INSDC center name

text: live

text: Generic

text: lymphocyte

text: Daudi

text: Burkitt's lymphoma

text: Homo sapiens

text: Sugano cDNA library, lymphocyte Daudi


characteristic: INSDC first public

text: live

text: Generic

text: dermoid cancer

text: Homo sapiens

text: Sugano cDNA library, dermoid cancer


text: live

text: Generic

text: fetal

text: Homo sapiens

text: brain

text: Sugano cDNA library, fetal brain


text: live

text: Generic

text: fetal

text: Homo sapiens

text: muscle

text: Sugano cDNA library, fetal muscle


text: live

text: Generic

text: hepatoma

text: Homo sapiens

text: liver

text: Sugano cDNA library, liver HepG2


text: live

text: Generic

text: embryo

text: Homo sapiens

text: kidney

text: Sugano cDNA library, embryo kidney


text: live

text: Generic

text: Homo sapiens

text: hippocampus

text: Sugano cDNA library, hippocampus


text: live

text: Generic

text: epithelial cell

text: Homo sapiens

text: kidney

text: Sugano cDNA library, kidney epithelial cell


text: UC Davis, Department of Pomology

text: live

text: Generic

text: Tulare

characteristic: cultivar

text: Walnut nut samples were harvested from Tulare trees growing in the 'Stuke Block' in the Wolfskill experimental orchard located in Winters, California (USA). Samples were harvested on August 1, 2001 between 8 and 10 am. Samples were then dissected the same day. Seed coat (pellicle) tissue was separated from embryos and frozen immediately in liquid nitrogen and stored at -80C. A gram of sample was removed and ground to a fine powder in liquid nitrogen. Total RNA was extracted using the hot borate procedure. Poly A+ RNA was obtained using the Poly(A) Purist kit (Ambion). The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). Primary library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes.

text: Mid season fruit collected Aug 1

characteristic: lab-host

text: Seed coat

characteristic: organ

text: Juglans regia

text: SfiIA

text: SfiIB

text: Hermaphrodite

characteristic: sex

text: Seed coat from mid-season walnut embryos collected Aug 1

text: Plasmid


characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: The library was constructed using mRNA from P. vulnus grown in vitro on walnut roots (Juglans regia x Juglans hindsii 'Paradox'). The culture plates had been inoculated four months prior to RNA isolation. cDNA was prepared using a CLONTECH SMART cDNA Library construction kit. The library was constructed by Monica Britton (mtbritton@ucdavis.edu) at USDavis, Pomology Department. Sequencing by: Washington University Genome Sequencing Center St. Louis.

text: mixed

text: Pratylenchus vulnus

text: 5': SFiIa

text: 3': SfiIb

text: Pratylenchus vulnus NEM1

text: plasmid

text: Lambda TriplEX2


text: live

text: Generic

text: The library was constructed using mRNA from P. vulnus grown in vitro on walnut roots (Juglans regia x Juglans hindsii 'Paradox'). The culture plates had been inoculated two months prior to RNA isolation. cDNA was prepared using a CLONTECH SMART cDNA Library construction kit. The library was constructed by Monica Britton (mtbritton@ucdavis.edu) at USDavis, Pomology Department. Sequencing by: Washington University Genome Sequencing Center St. Louis.

characteristic: description

text: mixed

text: Pratylenchus vulnus

characteristic: organism

text: 5': SFiIa

text: 3': SfiIb

text: Pratylenchus vulnus NEM3

characteristic: title

text: plasmid

text: Lambda TriplEX2

characteristic: vector


characteristic: External Id

text: live

text: Generic

text: Homo sapiens

text: heart

text: Sugano cDNA library, heart


text: live

text: Generic

text: Homo sapiens

text: small intestine

text: Sugano cDNA library, small intestine


text: live

text: Generic

text: endothelial cell

characteristic: cell type

text: Homo sapiens

text: umbilical vein

text: Sugano cDNA library, umbilical vein endothelial cell


text: live

text: Generic

text: Homo sapiens

text: muscle

characteristic: tissue-type

text: Sugano cDNA library, muscle


characteristic: INSDC center name

text: live

text: Generic

text: endothelial cell

text: Homo sapiens

text: Sugano cDNA library, endothelial cell


characteristic: INSDC first public

text: live

text: Generic

text: embryo

characteristic: dev-stage

text: Homo sapiens

text: brain

text: Sugano cDNA library, embryo brain


text: live

text: Generic

text: Homo sapiens

text: ileum

text: Sugano cDNA library, ileum


text: live

text: Generic

text: Homo sapiens

text: lung

text: Sugano cDNA library, lung


text: live

text: Generic

text: Homo sapiens

text: liver

text: Sugano cDNA library, liver


text: live

text: Generic

text: JTH

characteristic: cell-line

text: thyroid tumor

text: Homo sapiens

text: thyroid

text: Sugano cDNA library, thyroid JTH


text: live

text: Generic

text: signet-ring cell

text: KATOIII

text: signet-ring cell carcinoma

text: Homo sapiens

text: Sugano cDNA library, signet-ring cell KATOIII


text: live

text: Generic

text: Homo sapiens

text: kidney

text: Sugano cDNA library, kidney


text: live

text: Generic

text: glioma

text: Homo sapiens

text: Sugano cDNA library, KG-1-C


text: live

text: Generic

text: chronic myelogenous leukemia

text: Homo sapiens

text: bone marrow

text: Sugano cDNA library, bone marrow K562


text: live

text: Generic

text: fibroblast

text: normal

text: Homo sapiens

text: lung

text: Sugano cDNA library, lung fibroblast


text: The Institute for Genomic Research

text: live

text: Generic

text: T. vaginalis strain C1 amplified cDNA library constructed from high MW cDNA, made in lambda ZAPII. Inserts cloned unidirectionally in EcoRI and XhoI sites. Mass excision of library produced inserts in pBluescript-SK plasmid. Inserts sequenced from both 5' and 3' ends using M13F and T3 primers respectively.

text: Trichomonas vaginalis

text: EcoRI

text: XhoI

characteristic: strain

text: HMW C1 cDNA library

text: Phagemid

text: Lambda ZAPII


text: The Institute for Genomic Research

text: live

text: Generic

text: T. vaginalis strain T1 library constructed from cDNA, made in lambda TriplEx2. Normalized to contain less abundant clones compared to original T1 library. Inserts cloned unidirectionally in the SfiA and SfiB sites. Mass excision of library produced inserts in pTriplEx2 plasmid. Inserts sequenced from both 5' and 3' ends using TriplEx2 sequencing primer and polydT 24 bp primer respectively.

text: Trichomonas vaginalis

text: SfiA

text: SfiB

text: Cot6 normalized T1 cDNA library

text: Phagemid

text: Lambda TriplEx2


text: The Institute for Genomic Research

text: live

text: Generic

text: T. vaginalis strain T1 library constructed from cDNA, made in lambda TriplEx2. Normalized to contain less abundant clones compared to original Cot6 normalized T1 library. Inserts cloned unidirectionally in the SfiA and SfiB sites. Mass excision of library produced inserts in pTriplEx2 plasmid. Inserts sequenced from both 5' and 3' ends using TriplEx2 sequencing primer and polydT 24 bp primer respectively.

text: Trichomonas vaginalis

text: SfiA

text: SfiB

text: Cot1000 normalized T1 cDNA library

text: Phagemid

text: Lambda TriplEx2


text: The Institute for Genomic Research

text: live

text: Generic

text: T. vaginalis strain T1 library constructed from cDNA, made in lambda TriplEx2. Inserts cloned unidirectionally in the SfiA and SfiB sites. Mass excision of library produced inserts in pTriplEx2 plasmid. Inserts sequenced from both 5' and 3' ends using TriplEx2 sequencing primer and polydT 24 bp primer respectively.

text: Trichomonas vaginalis

text: SfiA

text: SfiB

text: non-normalized T1 cDNA library

text: phagemid

text: Lambda TriplEx2


text: live

text: Generic

text: macrophage

text: Homo sapiens

text: Sugano cDNA library, macrophage


text: live

text: Generic

text: OCUB-F

text: mammary gland tumor

text: Homo sapiens

text: mammary gland

text: Sugano cDNA library, mammary gland OCUB-F


text: live

text: Generic

text: Homo sapiens

text: pericardium

text: Sugano cDNA library, pericardium


text: live

text: Generic

text: Homo sapiens

text: placenta

text: Sugano cDNA library, placenta


text: live

text: Generic

text: Homo sapiens

text: pancreas

text: Sugano cDNA library, pancreas


text: live

text: Generic

text: Homo sapiens

text: prostata

text: Sugano cDNA library, prostata


text: live

text: Generic

text: Homo sapiens

text: rectum

text: Sugano cDNA library, rectum


text: Department of Physiology and Biophysics, University of Iowa

text: live

text: Generic

text: Trizol extracted mRNA; RT-PCR using the primers shown in EST file

text: thyroid

characteristic: organ

text: Papio anubis

text: baboon thyroid mRNA


text: University of Southern Queensland

characteristic: INSDC last update

text: live

text: Generic

characteristic: cultivar

text: Total RNA was isolated from seed embryos of wheat variety AUS1408 and Cascades, and reverse transcripted into first strand cDNA and used in differentail dispaly RT PCR. Anchor primer was CACT12CA, randon primer was CAGCCAACAC. The PCR product derived from AUS1408 was cloned into pGEM T-easy vector and sequenced.

text: 35 days after flowering

text: seed

text: Triticum aestivum

text: hermaphrodite

characteristic: sex

text: embryo

text: DDRT PCR product


text: The 2nd Hospital of Xi'an Jiaotong University

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Homo sapiens

text: Human acute leukemia Express Library

text: pMD18 T


text: Institute of Medical Science, University of Tokyo

text: live

text: Generic

text: primary epithelial cell

text: Homo sapiens

characteristic: organism

text: renal proximal tubule

text: Sugano cDNA library, renal proximal tubule primary epithelial cell

characteristic: title


characteristic: External Id

text: live

text: Generic

text: Homo sapiens

text: salivary gland

text: Sugano cDNA library, salivary gland


text: live

text: Generic

text: Homo sapiens

text: spleen

text: Sugano cDNA library, spleen


text: live

text: Generic

text: Homo sapiens

text: stomach mucosa

text: Sugano cDNA library, stomach mucosa


text: live

text: Generic

text: Homo sapiens

text: synovial membrane

text: Sugano cDNA library, synovial membrane


text: live

text: Generic

text: squamous cell

characteristic: cell type

text: well-differentiated squamous cell carcinoma

characteristic: description

text: Homo sapiens

text: Sugano cDNA library, squamous cell TE13


characteristic: INSDC center name

text: live

text: Generic

text: squamous cell

text: squamous cell carcinoma

text: Homo sapiens

text: Sugano cDNA library, squamous cell TE7


characteristic: INSDC first public

text: live

text: Generic

text: mammary gland tumor

text: Homo sapiens

text: mammary gland

characteristic: tissue-type

text: Sugano cDNA library, mammary gland T47D


text: live

text: Generic

text: Homo sapiens

text: thalamus

text: Sugano cDNA library, thalamus


text: live

text: Generic

text: fibroblast

text: TIG

characteristic: cell-line

text: fetal

characteristic: dev-stage

text: Homo sapiens

text: lung

text: Sugano cDNA library, fetal lung fibroblast TIG


text: live

text: Generic

text: lymphocyte

text: HTLV infected lymphoma

text: Homo sapiens

text: Sugano cDNA library, lymphocyte


text: live

text: Generic

text: Homo sapiens

text: thymus

text: Sugano cDNA library, thymus


text: live

text: Generic

text: Homo sapiens

text: testis

text: Sugano cDNA library, testis


text: live

text: Generic

text: Homo sapiens

text: umbilical cord

text: Sugano cDNA library, umbilical cord


text: live

text: Generic

text: Homo sapiens

text: uterus

text: Sugano cDNA library, uterus


text: live

text: Generic

text: neuroblast

text: SK-N-MC

text: neuroblastoma

text: Homo sapiens

text: Sugano cDNA library, neuroblast SK-N-MC


text: live

text: Generic

text: Homo sapiens

text: adipose tissue

text: Sugano cDNA library, adipose tissue


text: live

text: Generic

text: Homo sapiens

text: ilea mucosa

text: Sugano cDNA library, ilea mucosa


text: live

text: Generic

text: teratocarcinoma

text: Homo sapiens

text: Sugano cDNA library, NT-2


text: National Research Center for Genetic Engineering and Biotechnology

text: live

text: Generic

text: Vegetatively propagated plants were grown in growth chamber for 10 days watering every other day. Gene expression profile of drought-treated plants for 18 days were compared with that of well-watered plants. Using non-radioactive DD-AFLP method, ten differentially expressed cDNA bands corresponding to the internal fragments of expressed genes in leaf tissues were isolated.

text: 18 days of withholding water in pot culture grown in growth chamber.

text: Shoot

characteristic: organ

text: Aeluropus lagopoides

text: Leaf

text: Aeluropus lagopoides DD-AFLP method


text: University of British Columbia

characteristic: INSDC last update

text: live

text: Generic

text: Outer xylem from 5 year old trees harvested every two weeks between April and October of 2002 at the University of British Columbia south campus farm in Vancouver, British Columbia. mRNA was isolated from each tissue source independently and equal quantities of mRNA from each tissue were then pooled. cDNA was prepared from 5 micrograms of mRNA and directionally ligated into the pBluescript II SK (+) XR vector using the pBluescript II XR cDNA Library Construction Kit according to manufacturer's instructions with modifications (Stratagene). Plasmid DNA was then transformed by electroporation into DH10B cells (Invitrogen) for propagation.

text: E. coli DH10B T1 phage resistant cells

characteristic: lab-host

text: Populus trichocarpa

text: EcoRI (5' end of cDNA)

text: XhoI (3' end of cDNA)

text: Not determined

text: pBluescript II SK (+) XR

characteristic: vector


text: University of British Columbia

text: live

characteristic: INSDC status

text: Generic

characteristic: cultivar

text: Outer xylem from 5 year old trees harvested every two weeks between April and October of 2002 at the University of British Columbia south campus farm in Vancouver, British Columbia. mRNA was isolated from each tissue source independently and equal quantities of mRNA from each tissue were then pooled. cDNA was prepared from 5 micrograms of mRNA and directionally ligated into the pBluescript II SK (+) XR vector using the pBluescript II XR cDNA Library Construction Kit according to manufacturer's instructions with modifications (Stratagene). Plasmid DNA was then transformed by electroporation into DH10B cells (Invitrogen) for propagation. Normalization was applied according to published methods [Bonaldo M.F. et al. (1996) Genome Research 6(9):791] in order to reduce the abundance of highly expressed transcripts.

text: E. coli DH10B T1 phage resistant cells

text: Populus trichocarpa

text: EcoRI (5' end of cDNA)

text: XhoI (3' end of cDNA)

text: Not determined

characteristic: sex

text: pBluescript II SK (+) XR


text: University of British Columbia

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: 383-2499 (Nisqually-1)

text: Young and mature leaves, along with green stems, from 8 year old trees harvested within the Boise Cascade region of Washington state on May 15th, 2001. mRNA was isolated from each tissue source independently and equal quantities of mRNA from each tissue were then pooled. cDNA was prepared from 20 micrograms of mRNA according to the full-length cDNA library construction method described by Carninci P. et al. (2000), Genome Research 10(10):1617-1630 and directionally ligated into the pBluescript II SK (+) XR vector digested with SstI (5' end) and XhoI (3'). Plasmid DNA was then transformed by electroporation into DH10B cells (Invitrogen) for propagation.

text: E. coli DH10B T1 phage resistant cells

text: Populus trichocarpa

characteristic: organism

text: SstI (5' end of cDNA)

text: XhoI (3' end of cDNA)

text: Female

text: pBluescript II SK (+) XR


characteristic: External Id

text: University of British Columbia

text: live

text: Generic

text: Wild clone

text: Terminal vegetative buds from 20 year old trees harvested near Corvallis, Oregon on September 19th, 2001. cDNA was prepared from 5 micrograms of mRNA and directionally ligated into the pBluescript II SK (+) XR vector using the pBluescript II XR cDNA Library Construction Kit according to manufacturer's instructions with modifications (Stratagene). Plasmid DNA was then transformed by electroporation into DH10B cells (Invitrogen) for propagation.

text: E. coli DH10B T1 phage resistant cells

text: Populus trichocarpa

text: EcoRI (5' end of cDNA)

text: XhoI (3' end of cDNA)

text: Male

characteristic: title

text: pBluescript II SK (+) XR


text: University of British Columbia

characteristic: INSDC center name

text: live

text: Generic

text: Wild clone

text: Terminal vegetative buds from 20 year old trees harvested near Corvallis, Oregon on September 19th, 2001. cDNA was prepared from 5 micrograms of mRNA and directionally ligated into the pBluescript II SK (+) XR vector using the pBluescript II XR cDNA Library Construction Kit according to manufacturer's instructions with modifications (Stratagene). Plasmid DNA was then transformed by electroporation into DH10B cells (Invitrogen) for propagation. Normalization was applied according to published methods [Bonaldo M.F. et al. (1996) Genome Research 6(9):791] in order to reduce the abundance of highly expressed transcripts.

characteristic: description

text: E. coli DH10B T1 phage resistant cells

text: Populus trichocarpa

text: EcoRI (5' end of cDNA)

text: XhoI (3' end of cDNA)

text: Male

text: pBluescript II SK (+) XR


text: University of British Columbia

characteristic: INSDC first public

text: live

text: Generic

text: 383-2499 (Nisqually-1)

text: Phloem and cambium from 8 year old trees harvested within the Boise Cascade region of Washington state on May 15th, 2001. cDNA was prepared from 20 micrograms of mRNA according to the full-length cDNA library construction method described by Carninci P. et al. (2000), Genome Research 10(10):1617-1630 and directionally ligated into the pBluescript II SK (+) XR vector digested with SstI (5' end) and XhoI (3'). Plasmid DNA was then transformed by electroporation into DH10B cells (Invitrogen) for propagation.

text: E. coli DH10B T1 phage resistant cells

text: Populus trichocarpa

text: SstI (5' end of cDNA)

text: XhoI (3' end of cDNA)

text: Female

text: pBluescript II SK (+) XR


text: University of British Columbia

text: live

text: Generic

text: 383-2499 (Nisqually-1)

text: Outer xylem from 8 year old trees harvested within the Boise Cascade region of Washington state on May 15th, 2001. cDNA was prepared from 20 micrograms of mRNA according to the full-length cDNA library construction method described by Carninci P. et al. (2000), Genome Research 10(10):1617-1630 and directionally ligated into the pBluescript II SK (+) XR vector digested with SstI (5' end) and XhoI (3'). Plasmid DNA was then transformed by electroporation into DH10B cells (Invitrogen) for propagation.

text: E. coli DH10B T1 phage resistant cells

text: Populus trichocarpa

text: SstI (5' end of cDNA)

text: XhoI (3' end of cDNA)

text: Female

text: pBluescript II SK (+) XR


text: University of British Columbia

text: live

text: Generic

text: Sapling trees two metres in height and grown under greenhouse conditions were exposed to continuous feeding by Cryptorhynchus lapathi (poplar and willow borer) adults caged on the sapling using mesh bags. Bark (with phloem and cambium attached) from within the caged region was harvested 2 hours, 6 hours and 48 hours after the onset of treatment. mRNA was isolated from each tissue source independently and equal quantities of mRNA from each tissue were then pooled. cDNA was prepared from 5 micrograms of mRNA and directionally ligated into the pBluescript II SK (+) XR vector using the pBluescript II XR cDNA Library Construction Kit according to manufacturer's instructions with modifications (Stratagene). Plasmid DNA was then transformed by electroporation into DH10B cells (Invitrogen) for propagation.

text: E. coli DH10B T1 phage resistant cells

text: Populus trichocarpa x Populus nigra

text: EcoRI (5' end of cDNA)

text: XhoI (3' end of cDNA)

text: Male

text: pBluescript II SK (+) XR


text: University of British Columbia

text: live

text: Generic

text: Sapling trees two metres in height and grown under greenhouse conditions were exposed to continuous feeding by Cryptorhynchus lapathi (poplar and willow borer) adults caged on the sapling using mesh bags. Bark (with phloem and cambium attached) from within the caged region was harvested 2 hours, 6 hours and 48 hours after the onset of treatment. mRNA was isolated from each tissue source independently and equal quantities of mRNA from each tissue were then pooled. cDNA was prepared from 5 micrograms of mRNA and directionally ligated into the pBluescript II SK (+) XR vector using the pBluescript II XR cDNA Library Construction Kit according to manufacturer's instructions with modifications (Stratagene). Plasmid DNA was then transformed by electroporation into DH10B cells (Invitrogen) for propagation. Normalization was applied according to published methods [Bonaldo M.F. et al. (1996) Genome Research 6(9):791] in order to reduce the abundance of highly expressed transcripts.

text: E. coli DH10B T1 phage resistant cells

text: Populus trichocarpa x Populus nigra

text: EcoRI (5' end of cDNA)

text: XhoI (3' end of cDNA)

text: Male

text: pBluescript II SK (+) XR


text: Washington University School of Medicine

text: live

text: Generic

text: (pGEM-T Vector( from Promega) is ampicillin resistent, M13 forward and M13 reverse, or SP6 and T7 primers should be used for sequencing.)

text: Hydra oligactis

text: Hydra EST Kiel 6

text: plasmid

text: pGEM-T


text: Penn State University

text: live

text: Generic

text: This is a directionally cloned, non-normalized library. This library has been generated by the Floral Genome Project (FGP). The Floral Genome Project is funded by NSF's Plant Genome Research Program (DBI-0115684). More information about the project can be obtained at http://fgp.bio.psu.edu

text: SOLR

text: Asparagus officinalis

text: EcoRI

text: male inflorescences

characteristic: tissue-type

text: Phagemid

text: pBluescript SK (+/-)


text: University of Florida

text: live

text: Generic

text: Mitchell Diploid (aka. Mitchell, aka W115 in Europe)

text: supplier: Petunia x hybrida cv. Mitchell Diploid plants were grown from seeds to a fully flowering stage under standard greenhouse conditions. Flowers at anthesis stage were excised from plants grown in standard greenhouses and treated with 2ppm exogenous ethylene. Entire flowers were collected at 0, 5, 10, 15, 24, and 36 hours after treatment. Total RNA was extracted from each sample, and 100 micrograms of each sample was combined for subsequent poly A+ mRNA selection and cDNA synthesis.

text: lambda ZAPII unidirectional

text: Petunia x hybrida

text: EcoRI

text: XhoI

text: all floral organs

text: petunia floral post-ethylene cDNA library

text: pBluescript SK-


text: University of Florida

text: live

text: Generic

text: supplier: Petunia x hybrida cv. Mitchell Diploid plants were grown from seeds to a fully flowering stage under standard greenhouse conditions. Flowers at anthesis stage were self-pollinated and entire flowers were collected at 0, 5, 10, 24, 36 and 48 hours after pollination from plants grown in standard greenhouses. Total RNA was extracted from each sample, and 100 micrograms of each sample was combined for subsequent poly A+ mRNA selection and cDNA synthesis.

text: lambda ZAPII unidirectional

text: Petunia x hybrida

text: EcoRI

text: XhoI

text: all floral organs

text: petunia floral post-pollination cDNA library

text: pBluescript SK-


text: University of Florida

text: live

text: Generic

text: supplier: Petunia x hybrida cv. Mitchell Diploid plants were grown from seeds to a fully flowering stage under standard greenhouse conditions. Flowers at anthesis stage were self-pollinated and entire fruit (ovaries) were collected at 0, 1, 2, 3, 4, and 5 days after pollination from plants grown in standard greenhouses. Total RNA was extracted from each sample, and 100 micrograms of each sample was combined for subsequent poly A+ mRNA selection and cDNA synthesis.

text: lambda ZAPII unidirectional

text: Petunia x hybrida

text: EcoRI

text: XhoI

text: whole fruit (ovary)

text: petunia early fruit development cDNA library

text: pBluescript SK-


text: University of Florida

text: live

text: Generic

text: supplier: Petunia x hybrida cv. Mitchell Diploid plants were grown from seeds standard greenhouse conditions. Leaves were collected from several plants grown to a developmental stage just prior to floral initiation. Equal amounts (weight) of leaf tissue from unexpanded leaves from the upper 1/3 of the plant, expanded leaves from the middle 1/3 of the plant, and older leaves from the lower 1/3 of the plant were combined for subsequent RNA extraction and cDNA synthesis.

text: lambda ZAPII unidirectional

text: Petunia x hybrida

text: EcoRI

text: XhoI

text: leaves

text: petunia leaf cDNA library

text: pBluescript SK-


text: University of Florida

text: live

text: Generic

text: supplier: Petunia x hybrida cv. Mitchell Diploid plants were grown from seeds to a fully flowering stage under standard greenhouse conditions. Ten entire flowers of six developmental stages were collected on the same day from plants grown in standard greenhouses. The flower stages were as follows in chronological order from youngest to oldest: stage 1 - no color in corolla; corolla 0.5 inches long stage 2 - first sign of color in corolla; corolla .75-1 inches long) stage 3 - fully elongated corolla (not open); corolla 1.5 inches long stage 4 - fully open corolla; anthers not yet dehisced stage 5 - fully open corolla; freshly anthesed, bright yellow pollen; wet stigma stage 6 - pre-senescent; yellowing of corolla tube; dry brown pollen (if present); stigma dry. Total RNA was extracted from each sample, and 100 micrograms of each sample was combined for subsequent poly A+ mRNA selection and cDNA synthesis.

text: lambda ZAPII unidirectional

text: Petunia x hybrida

text: EcoRI

text: XhoI

text: all floral organs

text: petunia floral development cDNA library

text: pBluescript SK-


text: University of Florida

text: live

text: Generic

text: supplier: Petunia x hybrida cv. Mitchell Diploid plants were grown from seeds to a fully flowering stage under standard greenhouse conditions. Flowers at anthesis stage were self-pollinated and entire fruit (ovaries) were collected at 14, 16, 18, 20, 22, 24, and 26 days after pollination. Total RNA was extracted from each sample, and 100 micrograms of each sample was combined for subsequent poly A+ mRNA selection and cDNA synthesis.

text: lambda ZAPII unidirectional

text: Petunia x hybrida

text: EcoRI

text: XhoI

text: whole fruit (ovary)

text: petunia fruit ripening cDNA library

text: pBluescript SK-


text: Michigan State University

text: live

text: Generic

text: US H20, SR96(mixed population)

text: cDNA for library construction was synthesized by reverse transcription of mRNA of 3, 4, 5, 6 and 7 weeks after emergence of root. Three week old root enriched transcripts were obtained after subtraction of common transcripts with the 7 week old sample. 7 week old root enriched transcripts were obtained after subtraction of common transcripts with the 3 week old sample. Sequences that hybridized with the subtracted probes were submitted as three libraries, one with sequences that hybridized with 3 week enriched trancripts, one with sequences that hybridized with 7 week enriched transcripts, and one with sequences that hybridized to both.

text: Early Vegetative

characteristic: dev-stage

text: XL1-Blue MRF

text: Root

text: Beta vulgaris

text: Sugar Beet

characteristic: strain

text: Root

text: Young roots probed with 3 and 7 week old root enriched transcripts

text: plasmid

text: Uni-ZAP XR


text: Michigan State University

characteristic: INSDC last update

text: live

text: Generic

text: US H20, SR96(mixed population)

text: Early Vegetative

text: XL1-Blue MRF

text: Root

characteristic: organ

text: Beta vulgaris

text: Sugar Beet

text: Root

text: Young roots probed with 3 week old root enriched transcripts

text: plasmid

text: Uni-ZAP XR


text: Michigan State University

text: live

text: Generic

text: US H20, SR96(mixed population)

text: Early Vegetative

text: XL1-Blue MRF

characteristic: lab-host

text: Root

text: Beta vulgaris

text: Sugar Beet

text: Root

text: Young roots probed with 7 week old root enriched transcripts

text: plasmid

text: Uni-ZAP XR

characteristic: vector


text: Cold Spring Harbor Laboratory

text: live

characteristic: INSDC status

text: Generic

text: Cloned 5' RACE fragments amplified from 5' RACE cDNA generated from 15 pooled mouse tissues and stages: 7, 11, 15, & 17-day total embryo, whole brain, eye, kidney, liver, lung, prostate, submaxilary gland, smooth muscle, spleen, testes and uterus.

text: Mus musculus

text: Mouse 5' RACE clones

text: plasmid


text: Ludwig Institute for Cancer Research

text: live

text: Generic

text: A mini-library was made by cloning products derived from ORESTES PCR (U.S. Letters Patent application No. 196,716 - Ludwig Institute for Cancer Research) profiles into the pUC18 vector. Reverse transcription of tissue mRNA and cDNA amplification were performed under low stringency conditions.

text: Adult

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: National University of Ireland Galway (NUIG)

text: live

text: Generic

text: An Atlantic salmon heart cDNA library was constructed using the Lambda Zap Express/Gigapack cloning kit (Stratagene cloning systems). cDNA synthesis was carried out using an oligo-(dT) primer for the reverse transcription of 5ug of mRNA and the library was constructed by directional cloning EcoR1-Xho1 based on manufacturers instructions. An insert: vector ligation ratio of 1:5 was chosen as most optimum. The lambda library was packaged with Gigapack III gold packaging extracts and plated on the E. coli cell line XL1-Blue MRF'.

text: adult

text: heart

text: Salmo salar

text: heart

text: Atlantic salmon Lambda Zap Express heart cDNA library

text: Phage

text: Lambda ZAP Express


text: The Canadian Potato Genome Project - BioAtlantech

text: live

text: Generic

text: Shepody

characteristic: cultivar

text: supplier: Developmental series. Plants from pathogen-free Solanum tuberosum var. Shepody, clone 1756, nuclear stock were grown in a screenhouse under natural conditions. Mature, harvested tubers were stored in the dark at 9C, 95% relative humidity for 6 months. At that time, sprouting eyes were excised and pooled. The tuber skin was removed from tubers, the remaining tuber tissues pooled. RNA was isolated separately from the skinless tubers sprouting eyes. Equal amounts of RNA were pooled from the tubers and sprouts, and used for library construction.

text: Solanum tuberosum

text: EcoRI

text: XhoI

text: Sprouting Tubers

text: After-Cooking Darkening C

text: pBluescript II SK(+) XR


text: Gyeongsang National University

text: live

text: Generic

characteristic: NCBI submission model

text: Jangwon

text: Seedling

text: XL-1 Blue

text: Root

text: Brassica rapa subsp. pekinensis

text: Sal I

text: Not I

text: Chinese cabbage root library

text: Plasmid

text: pSPORT I


text: Centre National de Recherche Scientifique (CNRS)

text: live

text: Generic

characteristic: NCBI submission package

text: BY

characteristic: cell-line

text: Bright Yellow

text: Cell suspension cultures were treated with beta- or alpha-megaspermin for 1 or 5 hours. The expressed mRNA were analysed by a Differential Display-Reverse Transcript Polymerase Chain Reaction (DD-RTPCR) following the Clontech protocol.

text: Nicotiana tabacum

text: EcoR I

text: EcoR I

text: Cell suspension cultures

text: Tobacco ESTs characterized by a hypersensitive response specific pattern of expression library

text: Plasmid

text: pDrive (Qiagen)


characteristic: External Id

text: RIKEN Plant Science Center

text: live

text: Generic

text: Bright Yellow No.2

text: mRNA obtained from hormone treated BY-2 cells

text: Nicotiana tabacum

characteristic: organism

text: pBluescript II KS+


text: Zhongshan (Sunyetsun) University

characteristic: INSDC center name

text: live

text: Generic

text: Pseudourostyla cristata

text: Pseudourostyla cristata differentially expressed EST

characteristic: title


text: Penn State University

characteristic: INSDC first public

text: live

text: Generic

text: This is a directionally cloned, non-normalized library. This library has been generated by the Floral Genome Project (FGP). The Floral Genome Project is funded by NSF's Plant Genome Research Program (DBI-0115684). More information about the project can be obtained at http://fgp.bio.psu.edu

characteristic: description

text: SOLR

text: Asparagus officinalis

text: EcoRI

text: female inflorescences

text: Phagemid

text: pBluescript SK (+/-)


text: Penn State University

text: live

text: Generic

text: SOLR

text: Ribes americanum

text: EcoRI

text: young flower buds and inflorescences

characteristic: tissue-type

text: Phagemid

text: pBluescript SK (+/-)


text: Beijing Institute of Basic Medical Sciences

text: live

text: Generic

text: mature

characteristic: dev-stage

text: E.coli JM109

text: Rattus norvegicus

text: male

characteristic: sex

text: Wistar

characteristic: strain

text: spinal cord

text: Rat subtractive injured spinal cord cDNA library

text: Plasmid

text: T vector


text: Medical University of South Carolina

characteristic: INSDC last update

text: live

text: Generic

text: hemocytes

characteristic: cell type

text: Penaeus vannamei

text: EcoRI

text: EcoRI

text: Plasmid


text: Medical University of South Carolina

text: live

characteristic: INSDC status

text: Generic

text: hemocytes

text: Penaeus vannamei

text: EcoRI

text: EcoRI

text: LvB-HEMCdit

text: Plasmid

characteristic: vector


text: Medical University of South Carolina

text: live

text: Generic

characteristic: NCBI submission model

text: hemocytes

text: Penaeus vannamei

text: Sfi

text: Sfi

text: Plasmid

text: pTriplEX


text: Medical University of South Carolina

text: live

text: Generic

characteristic: NCBI submission package

text: hemocytes

text: Penaeus vannamei

text: Sfi

text: Sfi

text: LvE-stalk

text: Plasmid

text: pTriplEX


text: Medical University of South Carolina

text: live

text: Generic

text: hemocytes

text: Penaeus vannamei

text: EcoRI

text: EcoRI

text: LvG-gillidCt

text: Plasmid


text: Medical University of South Carolina

text: live

text: Generic

text: hemocytes

text: Penaeus vannamei

text: EcoRI

text: EcoRI

text: LvP-HPCdit

text: Plasmid


text: Medical University of South Carolina

text: live

text: Generic

text: hemocytes

text: Penaeus vannamei

text: EcoRI

text: EcoRI

text: LvP-HPidCt

text: Plasmid

text: pTriplEX


text: College of Life Science

text: live

text: Generic

text: larva

text: Musca domestica

text: Musca domestica larva


text: live

text: Generic

text: Shepody

characteristic: cultivar

text: supplier: Pathogen-challenge series. Tubers from pathogen-free Solanum tuberosum var. Shepody, clone 1756, were inoculated with Streptomyces scabei EF-35, by applying 1 ml of a spore stock (OD650 = 0.7). Tubers were placed in trays of sand and watered from the bottom for the remainder of the experiment. Inoculated tubers were sampled at 2 days, 1 week, 2 weeks and 4 weeks post-infection. Harvested tubers were washed in distilled water, blotted dry, cut into small pieces and frozen in liquid nitrogen. RNA was isolated from each sample collection, pooled and used for cDNA library construction.

characteristic: lab-host

text: Solanum tuberosum

text: EcoRI

text: XhoI

text: Tubers

text: Common Scab-Challenged Tubers

text: pBluescript II SK(+) XR


characteristic: External Id

text: live

text: Generic

text: Shepody

text: supplier: Developmental series. Plants from pathogen-free Solanum tuberosum var. Shepody, clone 1756, nuclear stock were grown in a screenhouse under natural conditions. Developing tubers of 10-15 mm in length and 30-40 mm in length were collected early during the growing season and pooled. Mature tubers were collected approximately 1 3/4 months later at top kill, and then collected two weeks later post-top kill and pooled. RNA was isolated from the 2 pooled collections and mixed for library construction. A normalized library was constructed following a modified protocol of Bonaldo et al. (1996. Genome Research 6: 791-806).

text: Solanum tuberosum

characteristic: organism

text: EcoRI

text: XhoI

text: Tubers

text: Developing Tubers

text: pBluescript II SK(+) XR


text: University of Agricultural Sciences

characteristic: INSDC center name

text: live

text: Generic

text: Finger millet plants of 20-day-old were subjected to water stress (20%F.C) by withholding water for one week and RNA isolated from leaf tissues. Subtracted cDNA library was made using Adapter ligation subtractive cloning method.

text: Three week old plant

text: Escherichia coli

text: Leaf

characteristic: organ

text: Eleusine coracana

text: EcoRI

text: EcoRI

text: Hermaphrodite

text: Leaf tissue

text: Finger millet stress induced cDNA library

characteristic: title


text: Universite de Lille 1

characteristic: INSDC first public

text: live

text: Generic

text: Hermes

text: mRNA were extracted from field grown plants (Dunkerque, France). Following harvest, roots, leaves and flowers were cut-off and discarded, as were the bottom 10 cm of the stem. The following bottom 15-cm section of the stem was then isolated and the outer fiber-bearing tissues peeled off and immediately frozen in liquid nitrogen. Library was constructed using ZAP-cDNA synthesis kit and ZAP-cDNA Gigapack III cloning kit (Stratagene).

characteristic: description

text: Mid-flowering stage

text: Linum usitatissimum

text: XhoI

text: EcoRI

text: Outer fiber-bearing tissues

characteristic: tissue-type

text: Flax fiber enriched Lambda ZapII Library

text: Phage


text: live

text: Generic

text: Shepody

text: supplier: Developmental series. Plants from pathogen-free Solanum tuberosum var. Shepody, clone 1756, nuclear stock were grown in a screenhouse under natural conditions. Mature, harvested tubers were stored in the dark at 4C, 95% relative humidity for 3 months. RNA was isolated for library construction. A normalized library was constructed following a modified protocol of Bonaldo et al. (1996. Genome Research 6: 791-806).

text: Solanum tuberosum

text: EcoRI

text: XhoI

text: Tubers

text: Cold Sweetening B

text: pBluescript II SK(+) XR


text: The Canadian Potato Genome Project - BioAtlantech

text: live

text: Generic

text: Shepody

text: supplier: Developmental series. Plants from pathogen-free Solanum tuberosum var. Shepody, clone 1756, nuclear stock were grown in a screenhouse under natural conditions. Emerging, expanding and mature leaves were harvested along the length of the plant and pooled. RNA was isolated and used for library construction. A normalized library was constructed following a modified protocol of Bonaldo et al. (1996. Genome Research 6: 791-806).

text: Solanum tuberosum

text: EcoRI

text: XhoI

text: Leaves

text: Mixed Leaf

text: pBluescript II SK(+) XR


text: live

text: Generic

text: Shepody

text: supplier: Developmental series. Plants from pathogen-free Solanum tuberosum var. Shepody, clone 1756, nuclear stock were grown in a screenhouse under natural conditions. Developing floral buds were harvested at various sizes up to opening and pooled. RNA was isolated and used for library construction. A normalized library was constructed following a modified protocol of Bonaldo et al. (1996. Genome Research 6: 791-806).

text: Solanum tuberosum

text: EcoRI

text: XhoI

text: Floral buds

text: Mixed Floral

text: pBluescript II SK(+) XR


text: Alfred-Wegner-Institute for Polar and Marine Research

text: live

text: Generic

text: Samples for total RNA isolation were taken continous for 5 days after a salt shock treatment increasing salinity from 34 to 60 PSU. Total RNA extraktion was performed with RNAqueous (Ambion) and mRNA purification with polyA Purist (Ambion). Further steps were carried out as described in the Cloneminer Kit. cDNA size fractionation was carried out with CHROMA Spin-400 columns and additionally on a gel.

text: 1-5 days after osmotic shock

characteristic: dev-stage

text: Fragilariopsis cylindrus

text: Antarctic

characteristic: strain

text: Fragilariopsis CloneMiner cDNA library (Invitrogen)


text: Washington University School of Medicine

characteristic: INSDC last update

text: live

text: Generic

text: The library was made from mRNA isolated from total RNA using oligo dT cellulose. Total RNA was generated from mixed stage X. index extracted from soil around fig (Ficus carica) roots. Nematodes were cleaned by passing through a series of sieves. Pure X. index were obtained by hand sorting of nematodes. The library was made in the pDNR lib kit using a SMART Creator cDNA library kit (Clontech). cDNA was cloned directionally using the SfiI site in the pDNR LIB vector. The library was provided by John T. Jones (jjones@scri.sari.ac.uk) at SCRI, Nematology Department. Sequencing by: Washington University Genome Sequencing Center, St. Louis, MO.

text: mixed

text: Xiphinema index

text: 5': SFiIa

text: 3': SfiIb

text: mixed

characteristic: sex

text: Xiphinema index

text: plasmid

text: pDNR-LIB


text: live

characteristic: INSDC status

text: Generic

text: The library was made from mRNA isolated from total RNA using oligo dT cellulose. Total RNA was generated from mixed stage X. index extracted from soil around fig (Ficus carica) roots. Nematodes were cleaned by passing through a series of sieves. Pure X. index were obtained by hand sorting of nematodes. The library was constructed by Incyte Genomics by cloning cDNA directionally into in the pSPORT1 vector using SalI and NotI sites. The library was provided by John T. Jones (jjones@scri.sari.ac.uk) at SCRI, Nematology Department. Sequencing by: Washington University Genome Sequencing Center, St. Louis, MO.

text: mixed

text: GC10 (only ligation mix supplied)

text: Xiphinema index

text: 5': SalI

text: 3': NotI

text: mixed

text: Xiphinema index CSEQDA01

text: plasmid

characteristic: vector


text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: The library was made from mRNA isolated from total RNA using oligo dT cellulose. Total RNA was generated from mixed stage X. index extracted from soil around fig (Ficus carica) roots. Nematodes were cleaned by passing through a series of sieves. Pure X. index were obtained by hand sorting of nematodes. The library was constructed by Incyte Genomics by cloning cDNA directionally into in the pBluescript (SK+) vector using NotI and EcoRI sites. The library was provided by John T. Jones (jjones@scri.sari.ac.uk) at SCRI, Nematology Department. Sequencing by: Washington University Genome Sequencing Center, St. Louis, MO.

text: mixed

text: GC10 (only ligation mix supplied)

text: Xiphinema index

text: 5': NotI

text: 3': EcoRI

text: mixed

text: Xiphinema index CSEQDL01

text: plasmid

text: pBluescript SK+


text: Liverpool School of Tropical Medicine,

text: live

text: Generic

text: Glossina morsitans morsitans

text: fat body

text: SSH Library from Glossina morsitans morsitans fat body


text: Duke University

text: live

text: Generic

text: a Mimulus guttatus cDNA library

text: Erythranthe guttata

text: Mimulus guttatus library 1

text: Plasmid

text: Stratagene lambda Uni-ZAP XR


text: Duke University

text: live

text: Generic

text: IM62 is a highly inbred line propagated for > 10 generations by self-fertilization and derived from a predominantly outcrossing (~80%), annual population of M. guttatus located on Iron Mountain, Linn Co., Oregon. Plants were grown from seed in the greenhouses at Duke University and tissue for RNA extraction was collected from 1-2 month old plants. RNA was extracted using the standard protocol for the Qbiogene FastRNA Pro Green kit. cDNA was synthesized from total RNA using the BD Biosciences SMART PCR cDNA synthesis kit. No size selection was performed. Library was not normalized and cDNA was not directionally cloned. Titer of primary library was ~1000 cfu/ng vector (pGEM-T Easy). High quality start and High quality end are determined by phred score >= 20 for the final base, and an average phred score >= 20 for the final 10 bases. Sequence ends were determined using cross_match to remove vector, and with ends of average phred score <= 11 removed, and homopolymeric end sequences of phred score <= 15 removed. Contaminants (bacterial and fungal) were screened for and not found. Project support provided by NSF FIBR. Project website: www.mimulusevolution.org

characteristic: lab-host

text: Erythranthe guttata

text: tips of developing inflorescences, consisting of very young unopened floral buds (< 5mm long), floral meristems, and shoot apical meristems

text: Mimulus guttatus library 2

text: Plasmid

text: pGEM-T Easy


characteristic: External Id

text: Plant Genetics & Breeding, China Agricultural University

text: live

text: Generic

text: NONGDA 3338/2463

characteristic: cultivar

text: EST from wheat root and leaf differentially expressed between hybrid and its parents

text: vigorous tillering stage

text: Triticum aestivum

text: root, leaf

text: Triticum aestivum subtracted, clontech


text: Gyeongsang National University

characteristic: INSDC center name

text: live

text: Generic

text: Jangwon

text: Seedling

text: XL-1 Blue

text: Flower

text: Brassica rapa subsp. pekinensis

characteristic: organism

text: EcoRI

text: XhoI

text: Chinese cabbage flower library

text: Plasmid

text: pBluescript II KS(+)


text: CNRS UMR 8080

text: live

text: Generic

text: cDNA made by oligo-dT priming. Directionally cloned into SalI/NotI sites using the following 5' adaptor: tcgacccacgcgtccg and 3' adaptor: gactagttctagatcgcgagcggccgcccttttttttttttttt. Average insert size 1.3kb. Primary library, non-amplified. Library constructed by V. Gawantka, PhD. REF: Gawantka, V., Pollet, N., Delius, H., Vingron, M., Pfister, R., Nitsch, R., Blumenstock, C., and Niehrs, C. (1998) Gene expression screening in Xenopus identifies molecular pathways, predicts gene function and provides a global view of embryonic patterning. Mech. Dev. 77:95-141.

text: embryo

text: XL-1 Blue

text: embryo

characteristic: organ

text: Xenopus laevis

text: SalI

text: NotI

text: both

text: whole embryo

characteristic: title

text: phagemid

text: pBluescript KS+


text: CNRS UMR 8080

characteristic: INSDC first public

text: live

text: Generic

text: cDNA made by oligo-dT priming. Directionally cloned into EcoRI/NotI sites using the following 5' adaptor: gaattccccggg and 3' adaptor: agagaactagtgtcgacgcggccgcttttttttttttttttttt. Vector designed for mRNA injections (reference Lemaire et al. (1995) 81:85-94). Average insert size 1.6kb. Primary library, non-amplified. Library constructed by A. Glinka, PhD. REF: Glinka, et al. (1996) Combinatorial signaling by Xwnt-11 and Xnr3 in the organizer epithelium. Mech. Dev. 60:221-231.

characteristic: description

text: embryo

text: XL-1 Blue

text: embryo

text: Xenopus laevis

text: EcoRI

text: NotI

text: both

text: whole embryo

text: plasmid


text: National Institute of Genetics

text: live

text: Generic

text: leukocyte-like cell

characteristic: cell type

text: wild caught animal

text: adult

characteristic: dev-stage

text: Eptatretus burgeri

text: Eptatretus burgeri adult


characteristic: INSDC last update

text: live

text: Generic

text: third instar larva

text: Plutella xylostella

text: whole body

characteristic: tissue-type

text: immunized Plutella xylostella cDNA


text: National Autonomous University of Mexico

text: live

characteristic: INSDC status

text: Generic

text: Negro Jamapa 81

text: Total RNA was prepared from P. vulgaris leaves taken from plants 15 days old. Poly (A+) RNA was obtained from total RNA using oligo (dT) cellulose. Poly (A+) RNA was reverse transcribed and cloned in the Uni-Zap XR vector (Stratagene). Plasmids containing cDNAs were then excised from the Uni-Zap XR vector and propagated in SOLR cells. All sequences were searched for insert integrity. All sequences were searched for vector segments to check for insert integrity. The sequences contain a minimum of 90 high quality bases and have been trimmed to remove vector and adaptor sequences.

text: Leaves from 15 day old plants

text: SOLR

text: Leaves

text: Phaseolus vulgaris

text: EcoRI

text: XhoI

text: Phaseolus vulgaris leaf EST library

text: pBluescript SK(+)


text: live

text: Generic

text: Negro Jamapa 81

text: Total RNA was prepared from P. vulgaris nodules, 15 days post inoculation with Rhizobium tropici CIAT 899. Poly (A+) RNA was obtained from total RNA using oligo (dT) cellulose. Poly (A+) RNA was reverse transcribed and cloned in the Uni-Zap XR vector (Stratagene). Plasmids containing cDNAs were then excised from the Uni- Zap XR vector and propagated in SOLR cells. All sequences were searched for vector segments to check for insert integrity. The sequences contain a minimum of 90 high quality bases and have been trimmed to remove vector and adaptor sequences.

text: SOLR

text: Nodules

text: Phaseolus vulgaris

text: EcoRI

text: XhoI

text: Phaseolus vulgaris nodule EST library

text: pBluescript SK(+)

characteristic: vector


text: live

text: Generic

characteristic: NCBI submission model

text: Negro Jamapa 81

text: Total RNA was prepared from young (1.5-5 cm) and mature (15 cm) P. vulgaris pods. Poly (A+) RNA was obtained from total RNA using oligo (dT) cellulose. Poly (A+) RNA from young and mature pods was mixed in a 1:1 ratio prior to reverse transcription. RNA was reverse transcribed and cloned in the Uni-Zap XR vector (Stratagene). Plasmids containing cDNAs were then excised from the Uni-Zap XR vector and propagated in SOLR cells. All sequences were searched for vector segments to check for insert integrity. The sequences contain a minimum of 99 high quality bases and have been trimmed to remove vector and adaptor sequences.

text: Mixed stages, young (1.5-5 cm) and mature (15 cm) pods

text: SOLR

text: Pods

text: Phaseolus vulgaris

text: EcoRI

text: XhoI

text: Phaseolus vulgaris pod EST library

text: pBluescript SK(+)


text: Universidad Nacional Autonoma de Mexico

text: live

text: Generic

characteristic: NCBI submission package

text: Negro Jamapa 81

text: P. vulgaris seedlings were watered for 3 weeks with phosphate-free nutrient solution. Total RNA was prepared from seedlings showing symptoms of phosphate starvation. Poly (A+) RNA was obtained from total RNA using oligo (dT) cellulose. RNA was reverse transcribed and cloned in the Uni-Zap XR vector (Stratagene). Plasmids containing cDNAs were then excised from the Uni-Zap XR vector and propagated in SOLR cells. All sequences were searched for vector segments to check for insert integrity. The sequences contain a minimum of 90 high quality bases and have been trimmed to remove vector and adaptor sequences.

text: 3 week, phosphate-starved

text: SOLR

text: Roots

text: Phaseolus vulgaris

text: EcoRI

text: XhoI

text: Phaseolus vulgaris P- root EST library

text: pBluescript SK(+)


text: Gyeongsang National University

text: live

text: Generic

text: Jangwon

text: seedling

text: XL-1 Blue

characteristic: lab-host

text: Brassica rapa subsp. pekinensis

text: Sal I

text: Not I

text: NaCl treated Chinese cabbage seedling library

text: Plasmid


characteristic: External Id

text: live

text: Generic

text: Total RNA samples were prepared individually from each tissue, checked for quality, then pooled for construction and normalization of the cDNA library by Invitrogen. Plasmids were isolated and inserts end-sequenced by the Marine DNA Sequencing and Analysis Facility at the Center for Marine Functional Genomic Studies, Mount Desert Island Biological Laboratory (J.N. Forrest, Jr., Director). Traces were processed for submission to dbEST by trace2dbest software (Parkinson, Anthony and Blaxter, unpublished software). The project was supported by NIH Grant Number P20-RR-16463 from the INBRE Program of the National Center for Research Resources (P.H. Hand, PI).

text: Adult

text: Squalus acanthias

text: Rectal gland, kidney, brain, testis, ovary, gill, intestine, heart, spleen

text: Dogfish Shark Multiple Tissues, Normalized

text: pCMV Sport 6.1


text: The Natural History Museum

text: live

text: Generic

text: mRNA, extracted from uninfected 1742 (susceptible) snails was used as a template for RT-PCR with random primers to generate cDNA fragments. These were cloned and sequenced using M13F. Primer sequences were removed from sequence.

text: Brain

text: Biomphalaria glabrata

text: Hermaphrodite

characteristic: sex

text: NHM 1742

characteristic: strain

text: BgORESTES uninfected NHM 1742 Brain

text: pGEM


text: The Natural History Museum

characteristic: INSDC center name

text: live

text: Generic

text: mRNA, extracted from infected 1742 (susceptible) snails was used as a template for RT-PCR with random primers to generate cDNA fragments. These were cloned and sequenced using M13F. Primer sequences were removed from sequence.

text: Brain

text: Biomphalaria glabrata

characteristic: organism

text: Hermaphrodite

text: NHM 1742

text: BgORESTES infected NHM 1742 Brain

text: pGEM


text: The Natural History Museum

text: live

text: Generic

text: mRNA, extracted from unexposed 3017 (resistant) snails was used as a template for RT-PCR with random primers to generate cDNA fragments. These were cloned and sequenced using M13F. Primer sequences were removed from sequence.

text: Brain

characteristic: organ

text: Biomphalaria glabrata

text: Hermaphrodite

text: NHM 3017

text: BgORESTES unexposed NHM 3017 Brain

characteristic: title

text: pGEM


text: The Natural History Museum

characteristic: INSDC first public

text: live

text: Generic

text: mRNA, extracted from schistosome-exposed 3017 (resistant) snails was used as a template for RT-PCR with random primers to generate cDNA fragments. These were cloned and sequenced using M13F. Primer sequences were removed from sequence.

characteristic: description

text: Brain

text: Biomphalaria glabrata

text: Hermaphrodite

text: NHM 3017

text: BgORESTES schistosome-exposed NHM 3017 Brain

text: pGEM


text: The Natural History Museum

text: live

text: Generic

text: mRNA, extracted from infected 3032 (susceptible) snails was used as a template for RT-PCR with random primers to generate cDNA fragments. These were cloned and sequenced using M13F. Primer sequences were removed from sequence.

text: Biomphalaria glabrata

text: Hermaphrodite

text: NHM 3032

text: Headfoot

text: BgORESTES infected NHM 3032 Headfoot

text: pGEM


text: The Natural History Museum

text: live

text: Generic

text: ovotestis

text: Biomphalaria glabrata

text: Hermaphrodite

text: NHM 3032

text: BgORESTES infected NHM 3032 ovotestis

text: pGEM


text: The Natural History Museum

text: live

text: Generic

text: brain

text: Biomphalaria glabrata

text: Hermaphrodite

text: NHM 3032

text: BgORESTES infected NHM 3032 brain

text: pGEM


text: The Natural History Museum

text: live

text: Generic

text: mRNA, extracted from uninfected 3032 (susceptible) snails was used as a template for RT-PCR with random primers to generate cDNA fragments. These were cloned and sequenced using M13F. Primer sequences were removed from sequence.

text: Biomphalaria glabrata

text: Hermaphrodite

text: NHM 3032

text: haemocytes

text: BgORESTES uninfected NHM 3032 haemocytes

text: pGEM


text: The Natural History Museum

text: live

text: Generic

text: Biomphalaria glabrata

text: Hermaphrodite

text: NHM 3032

text: haemocytes

text: BgORESTES infected NHM 3032 haemocytes

text: pGEM


text: The Natural History Museum

text: live

text: Generic

text: Biomphalaria glabrata

text: Hermaphrodite

text: NHM 3032

text: headfoot

text: BgORESTES uninfected NHM 3032 headfoot

text: pGEM


text: The Natural History Museum

text: live

text: Generic

text: ovotestis

text: Biomphalaria glabrata

text: Hermaphrodite

text: NHM 3032

text: BgORESTES uninfected NHM 3032 ovotestis

text: pGEM


text: The Natural History Museum

text: live

text: Generic

text: brain

text: Biomphalaria glabrata

text: Hermaphrodite

text: NHM 3032

text: BgORESTES uninfected NHM 3032 brain

text: pGEM


text: live

text: Generic

text: The library was constructed according Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. Denatured RNA was size fractionated on a 1% agarose gel.First strand cDNA synthesis was primed with oligo-dT primer containing a Not I site. Double strand cDNA was size selected according to mRNA size fraction, ligated with EcoR I adaptor, digested with NotI and then cloned directionally into pYX-Asc vector. The library tag sequence located between the Not I site and the polyA tail is AATAATTACG. This library was created for the University Iowa Brain Anatomy Project (BMAP): 'Gene Discovery in the Developing Mouse Nervous System', supported by National Institute of Mental Health (NIMH).

text: newborn 1,5,15 and embryos 15,16,17,18 dpc

characteristic: dev-stage

text: DH10B (T1 phage resistant)

text: Eye

text: Mus musculus

text: EcoR I

text: Not I

text: whole eye

text: Plasmid

text: pYX-Asc


text: Nankai University

characteristic: INSDC last update

text: live

text: Generic

text: MHPA induced; cultivated for 82 hours

text: Glycine max

text: root

text: Glycine max MHPA induced


text: National Eye Institute

text: live

text: Generic

text: Approximately 40ug total RNA was extracted from 7 adult human keratoconus corneas. A directionally cloned cDNA library in the pSPORT1 vector (Invitrogen) was constructed at Bioserve Biotechnology (Laurel MD) essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. cDNA was cloned in Not I/Sal I sites. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC).

text: Adult

text: Eye

text: Homo sapiens

text: Cornea

characteristic: tissue-type

text: Human keratoconus cornea, unamplified, (od/oe)

text: Plasmid


text: The Royal Veterinary & Agricultural University (KVL)

text: live

characteristic: INSDC status

text: Generic

text: XL1-Blue MRF'

text: Sus scrofa

text: Xho I

text: EcoR I

text: frontal cortex

text: rpfco

text: plasmid

text: pBluescript II SK (+)


text: live

text: Generic

text: XL1-Blue MRF'

text: Sus scrofa

text: Xho I

text: EcoR I

text: skin

text: rski

text: plasmid

text: pBluescript II SK (+)

characteristic: vector


text: live

text: Generic

characteristic: NCBI submission model

text: XL1-Blue MRF'

text: Sus scrofa

text: Xho I

text: EcoR I

text: jejunum

text: rjej

text: plasmid

text: pBluescript II SK (+)


text: live

text: Generic

characteristic: NCBI submission package

text: XL1-Blue MRF'

text: Sus scrofa

text: Xho I

text: EcoR I

text: Musculus biceps femoris

text: rpbfe

text: plasmid

text: pBluescript II SK (+)


text: live

text: Generic

text: XL1-Blue MRF'

characteristic: lab-host

text: Sus scrofa

text: Xho I

text: EcoR I

text: Oesophagus

text: rgul

text: plasmid

text: pBluescript II SK (+)


characteristic: External Id

text: live

text: Generic

text: Piglet 2 days post partum

text: XL1-Blue MRF'

text: Thymus

text: Sus scrofa

text: Xho I

text: EcoR I

text: rpty

text: plasmid

text: pBluescript II SK (+)


text: live

text: Generic

text: XL1-Blue MRF'

text: Heart

text: Sus scrofa

text: Xho I

text: EcoR I

text: rche

text: plasmid

text: pBluescript II SK (+)


text: live

text: Generic

text: XL1-Blue MRF'

text: Sus scrofa

text: Xho I

text: EcoR I

text: Mediastinum

text: rmed

text: plasmid

text: pBluescript II SK (+)


text: live

text: Generic

text: XL1-Blue MRF'

text: Sus scrofa

text: Xho I

text: EcoR I

text: Testicle

text: rcte

text: plasmid

text: pBluescript II SK (+)


text: live

text: Generic

text: XL1-Blue MRF'

text: Sus scrofa

text: Xho I

text: EcoR I

text: bladder

text: rdbla

text: plasmid

text: pBluescript II SK (+)


text: live

text: Generic

text: XL1-Blue MRF'

text: Sus scrofa

text: Xho I

text: EcoR I

text: Musculus triceps brachii

text: rptbr

text: plasmid

text: pBluescript II SK (+)


text: live

text: Generic

text: Foetus 107 days

text: XL1-Blue MRF'

text: Sus scrofa

text: Xho I

text: EcoR I

text: Brain stem

text: rfbs

text: plasmid

text: pBluescript II SK (+)


text: live

text: Generic

text: Foetus 50 days

text: XL1-Blue MRF'

text: Sus scrofa

text: Xho I

text: EcoR I

text: Epidermis

text: reep

text: plasmid

text: pBluescript II SK (+)


text: live

text: Generic

text: Foetus 107 days

text: XL1-Blue MRF'

text: Sus scrofa

text: Xho I

text: EcoR I

text: Cortex cerebri

text: rfcc

text: plasmid

text: pBluescript II SK (+)


text: live

text: Generic

text: Foetus 50 days

text: XL1-Blue MRF'

text: Sus scrofa

text: Xho I

text: EcoR I

text: Jejunum

text: reje

text: plasmid

text: pBluescript II SK (+)


text: live

text: Generic

text: XL1-Blue MRF'

text: Sus scrofa

text: Xho I

text: EcoR I

text: Amnion

text: ramn

text: plasmid

text: pBluescript II SK (+)


text: live

text: Generic

text: Foetus 50 days

text: XL1-Blue MRF'

text: Sus scrofa

text: Xho I

text: EcoR I

text: Eye

text: reye

text: plasmid

text: pBluescript II SK (+)


text: live

text: Generic

text: Foetus 50 days

text: XL1-Blue MRF'

text: Sus scrofa

text: Xho I

text: EcoR I

text: Brain stem

text: rebs

text: plasmid

text: pBluescript II SK (+)


text: live

text: Generic

text: XL1-Blue MRF'

text: Liver

text: Sus scrofa

text: Xho I

text: EcoR I

text: rpliv

text: plasmid

text: pBluescript II SK (+)


text: live

text: Generic

text: XL1-Blue MRF'

text: Sus scrofa

text: Xho I

text: EcoR I

text: Adrenal gland

text: rcad

text: plasmid

text: pBluescript II SK (+)


text: live

text: Generic

text: Foetus 107 days

text: XL1-Blue MRF'

text: Sus scrofa

text: Xho I

text: EcoR I

text: Hippocampus

text: rfhi

text: plasmid

text: pBluescript II SK (+)


text: live

text: Generic

text: XL1-Blue MRF'

text: Sus scrofa

text: Xho I

text: EcoR I

text: Colon ascendens

text: rcol

text: plasmid

text: pBluescript II SK (+)


text: live

text: Generic

text: XL1-Blue MRF'

text: Sus scrofa

text: Xho I

text: EcoR I

text: Musculus longissimus dorsi

text: rpldo

text: plasmid

text: pBluescript II SK (+)


text: live

text: Generic

text: XL1-Blue MRF'

text: Heart

text: Sus scrofa

text: Xho I

text: EcoR I

text: Left ventricle

text: rhlv

text: plasmid

text: pBluescript II SK (+)


text: live

text: Generic

text: XL1-Blue MRF'

text: Sus scrofa

text: Xho I

text: EcoR I

text: Musculus masseter

text: rmas

text: plasmid

text: pBluescript II SK (+)


text: live

text: Generic

text: XL1-Blue MRF'

text: uterus

text: Sus scrofa

text: Xho I

text: EcoR I

text: rute

text: plasmid

text: pBluescript II SK (+)


text: Duke University

text: live

text: Generic

text: cDNAs derived via differential display of mRNA extracted from livers of male and female adult Fundulus heteroclitus caught in a contaminated and a reference estuary in southeastern Virginia

text: adult

text: Fundulus heteroclitus

text: not identified

characteristic: sex

text: liver

text: Differential display-identified cDNAs from Fundulus heteroclitus liver


text: National Institute of Agrobiological Sciences

text: live

text: Generic

text: adult

text: Sus scrofa

text: mesenteric lymph node

text: full-length enriched swine cDNA library, adult mesenteric lymph node


text: live

text: Generic

text: adult

text: Sus scrofa

text: trachea

text: full-length enriched swine cDNA library, adult trachea


text: live

text: Generic

text: adult

text: Sus scrofa

text: testis

text: full-length enriched swine cDNA library, adult testis


text: National key lab of crop genetic improvement

text: live

text: Generic

text: Mo17 and TL94B

characteristic: cultivar

text: suppression subtracted hybridization (SSH) method.

text: seedling stage

text: Zea mays

text: root

text: SSH-cDNA differential expression Library related to aluminum stress

text: plasmid

text: T-easy vector