text: live

text: Generic

text: Barke

text: Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 800 bp

text: grown on filter paper at 25 Grad C for 48 hours in petridish, 1-1.5 cm in length

text: Hordeum vulgare subsp. vulgare

text: EcoRI (5'-end of cDNA)

text: XhoI (3'-end of cDNA)

text: roots

text: HR

characteristic: title

text: pBluescript SK+


status: PUBLIC

characteristic: External Id

text: Institute of Plant Genetics and Crop Plant Research (IPK)

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Barke

tag: attribute

characteristic: cultivar

text: Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 850 bp

characteristic: description

text: seedlings grown in greenhouse for 6 days

characteristic: dev-stage

characteristic: lab-host

text: Hordeum vulgare subsp. vulgare

characteristic: organism

text: EcoRI (5'-end of cDNA)

text: XhoI (3'-end of cDNA)

text: epidermis

characteristic: tissue-type

text: HP

text: pBluescript SK+

characteristic: vector


text: live

text: Generic

text: Barke

text: Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.

text: male inflorescences (approx. 2 mm in size), green anther stage

text: Hordeum vulgare subsp. vulgare

text: EcoRI (5'-end of cDNA)

text: XhoI (3'-end of cDNA)

text: male inflorescences

text: HM

text: pBluescript SK+


text: Leibniz Institute of Plant Genetics and Crop Plant Research (IPK)

text: live

text: Generic

text: Barke

text: pericarp 0-7 DAP(days after pollination). Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 900 bp

text: 0-7 DAP (days after pollination)

text: Hordeum vulgare subsp. vulgare

text: EcoRI (5'-end of cDNA)

text: XhoI (3'-end of cDNA)

text: pericarp

text: HZ

text: pBluescript SK+


text: live

text: Generic

text: Barke

text: apex (3-5 mm in size)

text: Hordeum vulgare subsp. vulgare

text: EcoRI (5'-end of cDNA)

text: XhoI (3'-end of cDNA)

text: apex

text: HX

text: pBluescript SK+


text: live

text: Generic

text: Barke

text: Roots were grown for two days on filter paper at room temperature. Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 1 kb

text: germinating seeds (48-96 h)

text: Hordeum vulgare subsp. vulgare

text: EcoRI (5'-end of cDNA)

text: XhoI (3'-end of cDNA)

text: germinating seeds

text: HV

text: pBluescript SK+


text: live

text: Generic

text: Barke

text: Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 900 bp

text: grown for 6 days in growth chamber (harvested at daylight conditions)

text: Hordeum vulgare subsp. vulgare

text: EcoRI (5'-end of cDNA)

text: XhoI (3'-end of cDNA)

text: etiolated leaves

text: HE

text: pBluescript SK+


text: live

text: Generic

text: Golden Promise

text: Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 1 kb

text: callus (5-10 mm in diameter)

text: Hordeum vulgare

text: EcoRI (5'-end of cDNA)

text: XhoI (3'-end of cDNA)

text: callus

text: HD

text: pBluescript SK+


text: live

text: Generic

text: Barke

text: female inflorescences (approx. 3 mm in size)

text: Hordeum vulgare subsp. vulgare

text: EcoRI (5'-end of cDNA)

text: XhoI (3'-end of cDNA)

text: female inflorescences

text: HI

text: pBluescript SK+


text: live

text: Generic

text: Barke

text: Hordeum vulgare subsp. vulgare

text: EcoRI (5'-end of cDNA)

text: XhoI (3'-end of cDNA)

text: green leaves

text: HG

text: pBluescript SK+


text: live

text: Generic

text: Barke

text: developing caryopsis, 16-25 DAF(days after flowing) Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 940 bp

text: developing caryopsis, 16-25 DAF (days after flowering)

text: Hordeum vulgare subsp. vulgare

text: EcoRI (5'-end of cDNA)

text: XhoI (3'-end of cDNA)

text: caryopsis

text: HF

text: pBluescript SK+


text: live

text: Generic

text: Barke

text: germinating seeds, 16-48h. Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable. Average insert size is 1.2 kb

text: germinating seeds, 16-48 h

text: Hordeum vulgare subsp. vulgare

text: EcoRI (5'-end of cDNA)

text: XhoI (3'-end of cDNA)

text: germinating seeds

text: HU

text: pBluescript SK+


text: live

text: Generic

text: Barke

text: 0-16 hours after imbibition

text: Hordeum vulgare subsp. vulgare

text: EcoRI (5'-end of cDNA)

text: XhoI (3'-end of cDNA)

text: endosperm early

text: HT

text: pBluescript SK+


text: live

text: Generic

text: Barke

text: developing caryopsis, 8-15 DAP(days after pollination) Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.

text: 8-15 DAP (days after pollination)

text: Hordeum vulgare subsp. vulgare

text: EcoRI (5'-end of cDNA)

text: XhoI (3'-end of cDNA)

text: developing caryopsis

text: BC

text: pBluescript SK+


text: The Natural History Museum

text: live

text: Generic

text: Biphyllus lunatus

text: Biphyllus lunatus cDNA

text: pCRII


text: The Natural History Museum

text: live

text: Generic

text: Carabus granulatus

text: Carabus granulatus cDNA


text: The Natural History Museum

text: live

text: Generic

text: Cicindela campestris

text: Cicindela campestris cDNA

text: pCRII


text: The Natural History Museum

text: live

text: Generic

text: Curculio glandium

text: Curculio glandium cDNA

text: pCRII


text: The Natural History Museum

text: live

text: Generic

text: Meladema coriacea

text: Meladema coriacea cDNA


text: University of Georgia

text: live

text: Generic

text: Zea mays

text: Illuminated leaves and sheaths

text: CSU

text: Uni-Zap


text: University of Georgia

text: live

text: Generic

text: Sorghum halepense

text: EcoRI

text: XhoI

text: pHER

text: Plasmid

text: pBluescript


text: University of Georgia

text: live

text: Generic

text: Sorghum halepense

text: rhizomes

text: pRC

text: Plasmid

text: pBluescript


text: University of Georgia

text: live

text: Generic

text: Sorghum halepense

text: rhizomes

text: pSHR

text: Plasmid


text: Washington University School of Medicine

text: live

text: Generic

text: The library was constructed by Claire Murphy and Dr. James McCarter at Washington University, St. Louis. Oligo(dT)-SL1 PCR based library. cDNA PCR products of size >400 nucleotides containing SL1 on the 5' end and oligo(dT) on the 3' end were non-directionally cloned into pCRII-TOPO(Invitrogen) following the Topo TA cloning protocol. Parasitic adult females were collected from naive animals and provided by Dr. Mark Viney of University of Bristol, Bristol, UK (Mark.Viney@bristol.ac.uk).

text: adult

text: Strongyloides ratti

text: EcoRI

text: EcoRI

text: Strongyloides ratti PA female naive SL1 TOPO v1

text: plasmid (kanamycin and ampicillin resistant)

text: pCRII-TOPO (Invitrogen)


text: Super Shrimp Inc.

text: live

text: Generic

text: The cDNA was isolated by mRNA differential display while comparing the RNA fingerprint of hepatopancreas tissue from healthy and white spot virus infected shrimp (Penaeus stylirostris)

text: Penaeus stylirostris

text: Hepatopancreas

text: Pacific blue shrimp mRNA differential clones


text: International Center for Tropical Agriculture, CIAT. http://www.ciat.cgiar.org

text: live

text: Generic

text: Total RNA was prepared from common bean leaves, poly(A) RNA was purified and reverse transcribed, and cDNAs were cloned into the pCMVSport6.0 vector (Life technologies) at the International Center for Tropical Agriculture (CIAT), Cali, COLOMBIA. The library was transformed into E. coli EMDH12S cells which were plated on Q-plates with Carbenicillin (100 mg/L). A Q-Bot was used to pick and array colonies into plates and filters. The clones were spotted, with a double replicate 4 x 4 pattern, onto gridded Hybond N+membrane filters containing 6 fields of 8 x 384-well plates each for a total of 48 plates per filter. The clones were sequenced from the 5' end using either SP6 or M13 Reverse primers, and then from the 3' end with the T7 or M13 Forward primers. Library plating and arraying, plasmid DNA preparations, DNA sequencing and sequence analysis were performed at CUGI (Munoz, Atkins ,Henry, Main, Frisch and Wing) and CIAT (Giraldo, Gaitan, Tohme and Blair). All sequences were searched for vector segments to check for insert integrity. The sequences contain a minimum of 100 high quality bases, with phred value of 20 or above, and have been trimmed to remove vector and adaptor sequences.

text: E. coli EMDH12S

text: Phaseolus vulgaris

text: Not I

text: Sal I

text: Leaf

text: Phaseolus vulgaris leaf EST library PV_GEa

text: Plasmid


text: live

text: Generic

text: The library was constructed by Claire Murphy and Dr. James McCarter at Washington University, St. Louis. Oligo(dT)-SL1 PCR based library. cDNA PCR products of size >400 nucleotides containing SL1 on the 5' end and oligo(dT) on the 3' end were non-directionally cloned into pCRII-TOPO(Invitrogen) following the Topo TA cloning protocol. Adult nematodes were harvested from infected dogs by Dr. Prema Arasu of North Carolina State University, Raleigh, NC (Prema_Arasu@ncsu.edu). Total RNA was isolated by Merry McLaird of ivergence, Inc., St. Louis,MO.

text: adult

text: Dirofilaria immitis

text: EcoRI

text: EcoRI

text: Dirofilaria immitis adult SL1 TOPO v1

text: pCRII-TOPO (Invitrogen)


text: US Department of Agriculture, Agriculture Research Service, Pacific West Area, Western Regional Research Center

text: live

text: Generic

text: Chinese Spring

text: Plants were grown until full tillering stage and root tissue was collected at Texas Tech Univeristy (Zhang, HT Nguyen Lab). Total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript SK(-) phagemids in the TJ Close lab (Close, Fenton) at the University of California, Riverside. Colony plating, plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

text: Full tillering

text: E. coli SOLR

text: Triticum aestivum

text: EcoRI

text: XhoI

text: Roots

text: Wheat unstressed root cDNA library

text: Phagemid

text: Lambda Uni-ZAP XR, excised phagemid pBluescript SK(-)


text: University of Delaware

text: live

text: Generic

text: E.coli XL-10 Blue

text: Gallus gallus

text: Male and Female

characteristic: sex

text: LPS-stimulated HD-11 macrophage cells

text: Chicken Macrophage cDNA library

text: pBluescript II-SK


text: All India Institute of Medical Sciences

text: live

text: Generic

text: mRNA differentially expressed between tumor and normal tissues of human esophagus.

text: Esophagus

characteristic: organ

text: Homo sapiens

text: Male

text: Squamous cell carcinoma

text: Human esophageal squamous cell carcinoma mRNA

text: Plasmid

text: pGEMT-Easy


text: Graduate School of Biotechnology, University of Korea

text: live

text: Generic

text: After the modified subtractive hybridization based on MOS (Nucleic Acids Research, Vol 28, No 20, e90), cDNA fragments were cloned by TA cloning method

text: fungal treatment & vegetative stage

text: Oryza minuta

characteristic: strain

text: leaf

text: Oryza minuta subtracted cDNA library

characteristic: title

text: Phagemid


characteristic: External Id

text: Universidade de Sao Paulo - USP - FCFRP

characteristic: INSDC center name

text: live

text: Generic

text: Paracoccidioides brasiliensis


text: Washington University School of Medicine

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: The library was prepared by Dr. Yulia Korshunova, at Washington University, St. Louis. The cDNA was made by using Dynabeads oligo-dT priming (Dynal). PCR based library using a modified protocol from the SMART PCR cDNA Synthesis kit from Clontech. Directionally cloned into UDG sites of pAMP1 vector. Average size of inserts ~0.35kb.

text: adult

text: Mus musculus

text: gut stem cells

text: Gordon Normalized Lib

text: amp resistent


text: The Institute for Genomic Research (TIGR; www.tigr.org)

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Kennebec or Binjte

text: supplier: Combination of untreated and Phytophthora infestans-treated libraries of stolons, leaves, leaflets, axillary buds of stem explants, petioles, germinating eyes, tubers, or roots.

text: SOLR

text: Solanum tuberosum

text: EcoRI

text: XhoI

text: mixed tissues

text: Generation of a set of potato cDNA clones for microarray analyses mixed potato tissues

text: pBluescript SK(-)


text: live

text: Generic

characteristic: NCBI submission package

text: The library was constructed by Claire Murphy and Dr. James McCarter at Washington University, St. Louis. Oligo(dT)-SL1 PCR based library. cDNA PCR products of size >400 nucleotides containing SL1 on the 5' end and oligo(dT) on the 3' end were non-directionally cloned into pCRII-TOPO(Invitrogen) following the Topo TA cloning protocol. Meloidogyne incognita eggs were provided by Andrew Kloek of Divergence Inc., St. Louis, MO.

text: egg

text: DH10B (Invitrogen)

text: Meloidogyne incognita

text: EcoRI

text: EcoRI

text: Meloidogyne incognita egg SL1 TOPO v1

text: plasmid (ampicillin and kanamycin resistant

text: pCRII-TOPO (Invitrogen)


text: Institute of Occupational Medicine

text: live

text: Generic

text: alveolar type II epithelial cels

characteristic: cell-line

text: ESTs from DDRT-PCR in alveolar type II epithelial cell transformation

characteristic: description

text: Rattus norvegicus

text: male

text: Lewis

text: Rat DDRT-PCR Express Library


text: TianJing Medical University

text: live

text: Generic

text: Homo sapiens

text: EcoRI

text: embryo

text: cerebrum cortex

text: Human embryo cerebrum cortex

text: plasmid

text: pGEM-T easy


text: College of Life Sciences

text: live

text: Generic

text: Rice seedlings were infested by brown planthopper. Seedling samples were collected 36 hour after infesting and mRNAs were used for construction of differentially expressed cDNA libraries by suppression subtractive hybridization

text: Two-week-old rice seeding infested by brown planthopper

characteristic: dev-stage

text: Oryza sativa

text: Minghui 63 susceptible variety to brown planthopper

text: Differentially expressed cDNA libraries of rice seedlings responding to brown planthopper feeding

text: pT-Adv


text: University of Edinburgh

text: live

text: Generic

text: Nippostrongylus brasiliensis is a nematode parasite of rodents (definitive host, rat) widely used in immunological research. The library was constructed from adult worms taken from the small intestine, 6 days after subcutaneous inoculation of infective larvae. cDNA was prepared by RT-PCR and directionally cloned in a plasmid vector. RT was primed with a tagged oligo-dT (NotI primer-adapter). This tag was then used to prime cDNA amplification together with a primer directed against an oligonucleotide previously ligated to the originally capped mRNA population. cDNA was ligated to SalI adapters, digested with NotI, and cloned into pSPORT 1 cut with SalI and NotI.

text: adult worm (day 6 post infection)

text: Nippostrongylus brasiliensis

characteristic: organism

text: SalI

text: NotI

text: mixed

text: Nippostrongylus brasiliensis adult (full length enriched)

text: Plasmid

text: pSPORT 1


text: Scottish Crop Research Institute

text: live

text: Generic

text: germinating cysts

text: Phytophthora infestans

text: Phytophthora infestans cDNA-AFLP fragment


text: University of Edinburgh

text: live

text: Generic

text: Constructed by Michelle Lizotte Waniewski for Rick Maizels, Edinburgh University, Edinburgh from mixed adult tissue. Primary library titre was 1x10^7 pfu/ml.

text: adult

text: Nippostrongylus brasiliensis

text: EcoRI

text: XhoI

text: mixed

text: Nippostrongylus brasiliensis amplified adult

text: Phage

text: Lambda Uni-ZAP XR


text: Washington University School of Medicine

text: live

text: Generic

text: The library was constructed by Claire Murphy and Dr. James McCarter at Washington University, St. Louis. Oligo(dT)-SL1 PCR based library. cDNA PCR products of size >400 nucleotides containing SL1 on the 5' end and oligo(dT) on the 3' end were non-directionally cloned into pCRII-TOPO(Invitrogen) following the Topo TA cloning protocol. RNA was provided by Andrew Kloek of Divergence, Inc., St. Louis, MO.

text: mixed stage

text: DH10B (Invitrogen)

characteristic: lab-host

text: Pratylenchus penetrans

text: EcoRI

text: EcoRI

text: Pratylenchus penetrans mixed stage SL1 TOPO v1

text: plasmid (ampicillin and kanamycin resistant)

text: pCRII-TOPO (Invitrogen)


text: Hiroshima University

text: live

text: Generic

text: blood cells

characteristic: cell type

text: adult

text: Ascidia sydneiensis samea

text: Ascidia sydneiensis samea blood cells cDNA


text: Research Institute for Microbial Diseases

text: live

text: Generic

text: germ cell

text: Mus musculus

text: testis

characteristic: tissue-type

text: Mus musculus transcript increased in spermiogenesis


text: The Institute for Genomic Research

text: live

text: Generic

text: cDNA made using Clontech SMART system description

text: schizont

text: Theileria parva

text: Muguga

text: plasmid

characteristic: vector


text: US Department of Agriculture, Agricultural Research Service

text: live

text: Generic

text: cDNA

text: Hordeum vulgare subsp. vulgare

text: leaf

text: cMWG

text: Plasmid

text: pBluescript


text: live

text: Generic

text: Steptoe

characteristic: cultivar

text: cDNA

text: Hordeum vulgare subsp. vulgare

text: malt

text: ABC


text: live

text: Generic

text: Willis

text: cDNA

text: Hordeum vulgare subsp. vulgare

text: etiolated leaf

text: BCD

characteristic: title


characteristic: External Id

text: live

text: Generic

text: Brooks

text: cDNA

text: Avena sativa

text: etiolated leaf

text: CDO


text: National Institute on Aging/National Institutes of Health

characteristic: INSDC center name

text: live

text: Generic

text: This clone is among a rearrayed set of 7,407 clones from more than 20 cDNA libraries.

text: mixed

text: Mus musculus

text: SalI

text: NotI

text: mixed

characteristic: sex

characteristic: strain

text: NIA Mouse 7.4K cDNA Clone Set

text: plasmid (ampicillin resistant)


text: Dalhousie University

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: 1st strand cDNA primed with (GA)10ACTAGTCTCGAG(T)18, followed by second strand synthesis, and ligated to 5' adapter (5')-aattcggcacgag- 3', 3'-gccgtgctc-5'. cDNA was cloned directionally (EcoRI/XhoI) into Stratagene Zap express lambda phage arms. Mass invivo excision done to obtain inserts in pBluescript SK(+/-) phagemid.

text: EII

text: E. coli SOLR

text: Artemia franciscana

text: EcoRI

text: XhoI

text: Mixed male and female

text: Whole organism

text: Artemia EII Stage, Stratagene (cat. 200450)

text: phagemid

text: pBluescript SK(+/-)


text: The Norwegian School of Veterinary Science

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Randomly cloned third differential products (DP3) of a Representational Differential Analysis (RDA) with bovine corpus luteum as tester tissue and skeletal muscle as driver.

text: Days 8-12 after ovulation

text: ovary

characteristic: organ

text: Bos taurus

text: female

text: corpus luteum

text: corpus luteum, RDA

text: Plasmid

text: pGEM-T Easy


text: Volcani Center

text: live

text: Generic

text: Subtraction library

characteristic: description

text: Infective juveniles (IJ3)

characteristic: dev-stage

text: Galleria mellonella

text: Steinernema feltiae

characteristic: organism

text: Infective juveniles

text: Steinernema feltiae IS6 - desiccation stress related ESTs

text: pGEM-T


text: live

text: Generic

text: The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. Denatured mRNa was size fractionated on a 1% agarose gel. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was size selected according to mRNA size fraction, ligated with EcoR I adaptor, digested with Not I, and then cloned directionally into pYX-Asc vector. The library tag sequence located between the Not I site and the polyA tail, is TGAGAGAGCC. This library was created for the University of Iowa Mouse Brain Molecular Anatomy Project (BMAP): 'Gene Discovery in the Developing Mouse Nervous System', supported by National Instututes of Mental Health (NIMH), Hemin Chin, Ph.D., program coordinator.

text: embryo 12.5 dpc

text: DH10B (T1 phage resistant)

characteristic: lab-host

text: brain

text: Mus musculus

text: EcoR I

text: Not I

text: whole brain

characteristic: tissue-type

text: Plasmid

text: pYX-Asc


text: INRA

text: live

text: Generic

text: ecotype: Landes; The library was made from the aerial part (above the collar) of 6 weeks old seedlings grown in hydroponic conditions. A mixture of genotypes were used. Oligo-dT primed cDNA was directionally cloned into the EcoRI-XhoI lambda-ZAP vector arms and mass-excised to form a pBluescript phagemid

text: 6 weeks old seedling

text: SOLR

text: Pinus pinaster

text: shoots

text: AN

text: Uni-ZAP XR

characteristic: vector


text: INRA

text: live

text: Generic

text: ecotype: Landes; The library was made from the aerial part (above the collar) of 6 weeks old seedlings grown in hydroponic conditions. A three weeks drought stress treatment was applied by lowering the osmotic potential of the nutrient solution to -0.45 MPa using 170 g/l of polyethylene glycol as an osmoticum. A mixture of genotypes were used. Oligo-dT primed cDNA was directionally cloned into the EcoRI-XhoI lambda-ZAP vector arms and mass-excised to form a pBluescript phagemid

text: 6 weeks old seedling

text: SOLR

text: Pinus pinaster

text: shoot

text: AS

characteristic: title

text: Uni-ZAP XR


characteristic: External Id

text: INRA

text: live

text: Generic

text: ecotype: Landes; The library was made from the roots of 6 weeks old seedlings grown in hydroponic conditions. A mixture of genotypes were used. Oligo-dT primed cDNA was directionally cloned into the EcoRI-XhoI lambda-ZAP vector arms and mass-excised to form a pBluescript phagemid

text: 6 weeks old seedling

text: SOLR

text: Pinus pinaster

text: root

text: RN

text: Uni-ZAP XR


text: INRA

characteristic: INSDC center name

text: live

text: Generic

text: ecotype: Landes; The library was made from the roots of 6 weeks old seedlings grown in hydroponic conditions. A three weeks drought stress treatment was applied by lowering the osmotic potential of the nutrient solution to -0.45 MPa using 170 g/l of polyethylene glycol as an osmoticum. A mixture of genotypes were used. Oligo-dT primed cDNA was directionally cloned into the EcoRI-XhoI lambda-ZAP vector arms and mass-excised to form a pBluescript phagemid

text: 6 weeks old seedling

text: SOLR

text: Pinus pinaster

text: root

text: RS

text: Uni-ZAP XR


text: University of Cologne

text: live

text: Generic

text: tissue_lib: pACT2

text: Drosophila melanogaster

text: whole embryo

text: Drosophila melanogaster whole embryo 0-18hr


text: Southampton Univercity

text: live

text: Generic

text: Peripheral blood leukocytes

characteristic: cell type

text: Differential cell display

text: Homo sapiens

text: CFS leukocyte

text: Plasmid

text: pCR TOPO II


text: Bielefeld University

text: live

text: Generic

text: KWS2320 (double haploid, monogerm breeding line)

characteristic: cultivar

text: cDNA library from sugar beet, library provided by KWS Kleinwanzlebener Saatzucht AG Einbeck, Germany, contact: b.schulz@kws.de; cloning sites SalI-NotI, primer sites and orientation: SP6-SalI-CCACGCGTCCG-5prime-cDNA-polyA-CC-NotI-T7; Note: Sequencing granted in the context of the GABI-Beet project, local PI: Dr. Katharina Schneider, coordinator: Prof. Christian Jung; Sequence submission managed by RZPD/GABI-Primary database: http://gabi.rzpd.de

text: Beta vulgaris

text: SalI

text: NotI

text: inflorescence


text: Bielefeld University

characteristic: INSDC first public

text: live

text: Generic

text: cDNA library from sugar beet, library provided by KWS Kleinwanzlebener Saatzucht AG Einbeck, Germany, contact: b.schulz@kws.de; cloning sites SalI-NotI, primer sites and orientation: SP6-SalI-CCACGCGTCCG-5prime-cDNA-polyA-CC-NotI-T7; Note: Sequencing granted in the context of the GABI-Beet project, local PI: Dr. Katharina Schneider, coordinator: Prof. Christian Jung; Sequence submission managed by RZPD/GABI-Primary database:http://gabi.rzpd.de

text: Beta vulgaris

text: SalI

text: NotI

text: leaf


text: Bielefeld University

characteristic: INSDC last update

text: live

text: Generic

text: Beta vulgaris

text: SalI

text: NotI

text: storage root

text: MPIZ-ADIS-024-storage root


text: Bielefeld University

text: live

characteristic: INSDC status

text: Generic

text: Beta vulgaris

text: SalI

text: NotI

text: developing root

text: MPIZ-ADIS-024-developing root


text: Basic Medical Sciences

text: live

text: Generic

characteristic: NCBI submission model

text: Homo sapiens

text: pancreas; fetal brain

text: Homo sapiens pancreas and fetal brain


text: Iowa State University

text: live

text: Generic

characteristic: NCBI submission package

text: The MI-P-E7 library is derived from the following tissue(s): preelongation day 12 conceptus. For a detailed description of the library from which this clone was derived, please visit our web site at http://pigest.genome.iastate.edu/.

text: DH10B (Life Technologies)

text: Sus scrofa

text: Not I

text: EcoRI

text: crossbreed

characteristic: strain


text: Iowa State University

text: live

text: Generic

text: The MI-P-HO library is a normalized library derived from hypothalamus at estrus days 0 and 12 and ovary at estrus days 0, 5 and 12. For a detailed description of the library from which this clone was derived, please visit our web site at http://pigest.genome.iastate.edu/.

characteristic: description

text: DH10B (Life Technologies)

text: Sus scrofa

text: Not I

text: EcoRI

text: crossbreed

text: MI-P-HO


text: Iowa State University

text: live

text: Generic

text: The MI-P-NF library is a normalized library derived from fetus at gestational days 20 and 45. Some clones from this library may contain inserts from library MI-P-E2 fetus gestational day 20. For a detailed description of the library from which this clone was derived, please visit our web site at http://pigest.genome.iastate.edu/.

text: DH10B (Life Technologies)

text: Sus scrofa

text: Not I

text: EcoRI

text: crossbreed

text: MI-P-NF


text: Institute of Materia Medica, Di Ao Pharmaceutical Group, Chengdu, China

text: live

text: Generic

text: epithelial cell

text: Breast cancer cell MDA-MB-231

characteristic: cell-line

text: Forward suppression subtractive hybridization (SSH) was performed on MDA-MB-231 breast cancer cells treated or untreated with a new compound for 1 day used Clontech PCR-Select cDNA subtraction kit. The subtractive product was cloned into pGEM-T Easy vector. Clontech PCR-Select differential kit was used to verify the cDNA clones whose expression increased after compound treating.

text: Breast

characteristic: organ

text: Homo sapiens

text: Rsa

text: Rsa

text: Forward subtracted library of breast cancer cell treated with a novel compound for 1 day

text: Plasmid

text: pGEM-T Easy


text: live

text: Generic

text: epithelial cell

text: Breast cancer cell MDA-MB-231

text: Forward Suppression subtractive hybridization (SSH) was performed on MDA-MB-231 breast cancer cells treated or untreated with a new compound for 7 days used Clontech PCR-Select cDNA subtraction kit.The subtractive products was cloned into pGEM-T Easy vector. Clontech PCR-Select differential kit was used to verify the cDNA clones whose expression increased after compound treating.

characteristic: lab-host

text: Breast

text: Homo sapiens

characteristic: organism

text: Rsa

text: Rsa

text: Forward subtracted library of breast cancer cell treated with a novel compound for 7 days

text: Plasmid

text: pGEM-T Easy


text: live

text: Generic

text: epithelial cell

text: Breast cancer cell MDA-MB-231

text: Reversed suppression subtractive hybridization (SSH) was performed on MDA-MB-231 breast cancer cells treated or untreated with a new compound for 7 days used Clontech PCR-Select cDNA subtraction kit.The subtractive products was cloned into pGEM-T Easy vector. Clontech PCR-Select differential kit was used to verify the cDNA clones whose expression decreased after compound treating.

text: Breast

text: Homo sapiens

text: Rsa

text: Rsa

text: Reverse subtracted library of breast cancer cell treated with a novel compound for 7 days

text: Plasmid

text: pGEM-T Easy

characteristic: vector


text: Carnegie Institution of Washington, Dept. of Plant Biology

text: live

text: Generic

text: Wyuna

text: developing endosperm tissue 8, 10 and 12 DPA (days post anthesis)

characteristic: dev-stage

text: Triticum aestivum

text: endosperm

characteristic: tissue-type

text: wheat EST endosperm library

characteristic: title


characteristic: External Id

text: Xiangya School of Medicine

characteristic: INSDC center name

text: live

text: Generic

text: adult

text: DH5 alpha

text: heart

text: Rattus norvegicus

text: male

characteristic: sex

text: myocardium

text: Rat heart ischemia/reperfusion subtracted cDNA library

text: plasmid

text: pGEM-T


text: University of Milano

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Cenchrus americanus

text: Inbred IP3088

text: In-vitro Germinating Pollen

text: Pearl Millet Pollen Tubes Lambda Zap Library

text: Lambda Zap


text: Sree Chitra Tirunal Institute for Medical Science and Technology

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Brain

text: Rattus norvegicus

text: Wister

text: Rat epileptic hippocampus


text: University of Illinois

text: live

text: Generic

characteristic: NCBI submission package

characteristic: cultivar

text: Stressed 24 hours at 150 mM NaCl

text: 2 weeks old

text: Zea mays

text: Roots

text: Salt stressed Zea mays roots cDNA library

text: pBluescript SK+


text: University of Illinois

text: live

text: Generic

text: Stressed 24 hours at 150 mM NaCl

characteristic: description

text: 2 weeks old

text: Zea mays

text: Leaves

text: Salt stressed Zea mays leaves cDNA library

text: pBluescript SK+


text: Chang Gung University

text: live

text: Generic

characteristic: cell-line

text: Trophozoites at mid-log phase

text: XL1 Blue-MRF'

text: Trichomonas vaginalis

text: EcoRI

text: XhoI

text: Tv30236_PT cDNA Library

text: Lambda ZAP-Express (Stratagene)


text: Universtiy of Maryland

text: live

text: Generic

text: Conidiobolus coronatus was grown in minimal medium supplemented with Manduca sexta cuticle and peptone for 18 hours. A cDNA library was constructed in the unidirectional Lambda vector UniZap.

text: Conidiobolus coronatus

text: ARSEF 512

characteristic: strain

text: Conidiobolus cornatus ARSEF 512

text: UniZap


text: National Eye Institute of China, Zhongshan Ophthalmic Center, Zhongshen(Sun Yat-Sen) University

text: live

text: Generic

text: The sequences were from the retina of retinal degeneration slow (rds) mouse during the development of retinitis pigmentosa by 5'-RACE experiments. The rds mouse and the normal control C3B mouse were purchased from the Jackson Laboratory (600 Main St., Bar Harbor, Maine 04609, USA).

characteristic: lab-host

text: Mus musculus

characteristic: organism

text: male and female

text: inbred congenic rds mouse (JR1999 C3H-Prph2Rd2)

text: Retina

text: cDNA fragments (5'end of RACE product) of the rds mouse

text: plasmid

text: pGEM-TEasy(Promega)


text: USDA - ARS

text: live

text: Generic

text: Ridge Pineapple

text: A high quality EST with at least 200 contiguous bases at Trace Tuner score of 20 or better

text: 50 days after germination

characteristic: dev-stage

text: Citrus sinensis

text: EcoRI

text: XhoI

text: entire seedling

text: Ridge pineapple sweet orange entire seedling

text: pBluescript II SK+

characteristic: vector


text: China Agricultural University

text: live

text: Generic

text: Triticum aestivum

text: young ear

characteristic: tissue-type

text: Triticum aestivum floret differentiation to anther separation

characteristic: title


characteristic: External Id

text: Washington University School of Medicine

characteristic: INSDC center name

text: live

text: Generic

text: ***WARNING: Subsequent examination of these samples has revealed the presence of an additional Trichostrongyloidea cattle nematode, Cooperia oncophora. Sequences in this library may derive from either Ostertagia or Cooperia.*** The library was constructed by Claire Murphy and Dr. James McCarter at Washington University, St. Louis. Oligo(dT)-SL1 PCR based library. Ostertagia ostertagi L3 cDNA PCR products of size >400 nucleotides containing SL1 on the 5' end and oligo(dT) on the 3' end were non-directionally cloned into pCRII-TOPO(Invitrogen) following the Topo TA cloning protocol. Nematodes were provided by Dr. Louis Gasbarree of the USDA, Beltsville, MD (lgasbarr@anri.barc.usda.gov). Third stage exsheathed larvae were collected from 14 day fecal-sphagnum moss cultures of Ostertagia eggs. The larvae were recovered by overnight passage on a Baermann apparatus, and then cleaned by passage through a 20 micron nylon mesh. The larvae were then subjected to a treatment with 1.25% chlorox to induce excystation. The larvae were washed with 5 changes of PBS and then pelleted and snap frozen in liquid nitrogen.

text: third stage exsheathed larvae

text: Ostertagia ostertagi

text: EcoRI

text: EcoRI

text: Ostertagia ostertagi L3 SL1 TOPO v2

text: plasmid (kanamycin and ampicillin resistant)

text: pCRII-TOPO (Invitrogen)


text: Nara Institute of Science and Technology

characteristic: INSDC first public

text: live

text: Generic

text: Golden Arrow

text: Zea mays

text: Zea mays cv. Golden Arrow


text: Tokyo University of Marine Science and Technology, Graduate School of Marine Science and Technology

characteristic: INSDC last update

text: live

text: Generic

text: leukocyte

characteristic: cell type

text: Paralichthys olivaceus

text: Paralichthys olivaceus leukocyte library


text: The University of Tokyo, Human Genome Center

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Homo sapiens

text: vestibule

text: Human vestibular cDNA library


text: Kyoto University

text: live

text: Generic

characteristic: NCBI submission package

text: 60 embryonic day

text: Bos taurus

text: Cloned bovine placenta cDNA


text: The Fourth Military Medical University

text: live

text: Generic

text: differentially expressed gene isolated from mice irradiateelly expressed by NEMP

characteristic: description

text: Mus musculus

text: BALB/c

text: hippocampus

text: Mus musculus hippocampus BALB/c differentially expressed


text: Vlaams Interuniversitair Instituut voor Biotechnologie (VIB)

text: live

text: Generic

text: Petit Havana SR1

characteristic: cultivar

text: Nicotiana tabacum

text: leaf

text: Nicotiana tabacum cv. Petit Havana SR1 leaf


text: National Eye Institute

text: live

text: Generic

text: Neural retina tissue was dissected from two 80 year old donors with no observed eye disease. 100ug of total RNA was used for library construction. A directionally cloned cDNA library in the pSPORT1 vector (Life Technologies) was constructed at Bioserve Biotechnology (Laurel MD) essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC).

text: Adult

text: Eye

characteristic: organ

text: Homo sapiens

text: Retina

text: Human Retina cDNA (Un-normalized, unamplified): hd/he

text: Plasmid


text: University of Bonn

text: live

text: Generic

text: cDNA fragments obtained from differential display RT-PCR banding patterns were cloned into pGEM

text: 8 cell stage embryo

text: Bos taurus

text: differential display RT-PCR from 8 cell preimplantation bovine embryos


text: University of Bonn

text: live

text: Generic

text: blastocyst cell stage embryo

text: Bos taurus

text: differential display RT-PCR from blastocyst cell preimplantation bovine embryos


text: University of Bonn

text: live

text: Generic

text: morula cell stage embryo

text: Bos taurus

text: differential display RT-PCR from morula cell preimplantation bovine embryos


text: University of Bonn

text: live

text: Generic

text: 16 cell stage embryo

text: Bos taurus

text: differential display RT-PCR from 16 cell preimplantation bovine embryos


text: University of Bonn

text: live

text: Generic

text: subtractive cDNA library was constructed using the PCR Select kit (Clontech)

text: Blastocyst

text: Bos taurus

text: subtractive cDNA libraries from blastocyst preimplantation bovine embryos


text: University of Bonn

text: live

text: Generic

text: Heterologous primer from porcine were used to amplify bovine target cDNA

text: Blastocyst stage embryo

text: Bos taurus

text: heterologous-PCR from blastocyst preimplantation bovine embryos


text: University of Bonn

text: live

text: Generic

text: 8 cell stage embryo

text: Bos taurus

text: heterologous-PCR from 8 cell preimplantation bovine embryos


text: Hainan University

text: live

text: Generic

text: Dunaliella salina

text: Dunaliella salina pGEM-T plasmid library


text: Beijing Agro-biotechnology Research Center

text: live

text: Generic

text: Total RNAs were isolated from pistils using Trizol reagent (Invitrogen, USA). Then, polyA+ mRNA was isolated using oligo(dT) cellulose as described. cDNA was synthesized using a lambda-zipLox cDNA synthesis kit(CAT No.19643-014, Invitrogen, USA). The phage library was converted through mass excision to a plasmid library in the vector pZL1. The plasmid library was plated on 15-cm LB agar plates with 100ug/mL ampicillin. Individual clones were picked at random and propagated. The 5'ends of the cDNA clones were sequenced on ABI Prism377 DNA sequencer.

text: flower

text: Prunus dulcis

text: Sal I

text: Not I

text: pistils

text: almond cDNA library

text: plasmid


text: UCSF

text: live

text: Generic

text: Homo sapiens

text: Homo sapiens library (Kratz CP)


text: live

text: Generic

text: astrocytes

text: Rattus norvegicus

text: astrocytes of rat


text: University of Georgia

text: live

text: Generic

text: cDNA library was prepared from mRNA isolated from the apical 2-3 cm of actively growing rhizomes of Johnson grass (Sorghum halepense) and cloned into the EcoRI/XhoI sites of lambda ZAP II (Stratagene).

text: Sorghum halepense

text: EcoRI

text: XhoI

text: rhizome

text: Sorghum halepense rhizome cDNA library

text: plasmid

text: pBluescript SK(-)


text: University of Georgia

text: live

text: Generic

text: PCR amplification products that appeared, based on size, to be specific to Sorghum halapense rhizomes were purified from agarose gels, re-amplified using a modified anchor primer with a terminal EcoRI site (EcoRIT12GT) and a modified decamer with a HindIII site (HindL1), then cloned into the EcoRI/HindIII sites of pBluescript.

text: Sorghum halepense

text: EcoRI

text: HindIII

text: rhizome

text: Sorghum halepense rhizome differential display library

text: plasmid

text: pBluescript SK(-)


text: Washington University School of Medicine

text: live

text: Generic

text: The library was constructed by Claire Murphy and Dr. James McCarter at Washington University, St. Louis. The cDNA was made by using Dynabead oligo-dT priming (Dynal). PCR based library using a modified protocol from the SMART PCR cDNA Synthesis Kit from Clontech. Directionally cloned into the UDG sites of pAMP1. Newborn larvae were collected from infected rats and provided by Dr. Judith Appleton of Cornell University, Ithaca, NY (jaa2@cornell.edu).

text: immature L1

characteristic: lab-host

text: Trichinella spiralis

text: NotI

text: SalI

text: Trichinella spiralis immature L1 pAMP1 v1

text: plasmid (ampicillin resistant)

text: pAMP1 (Gibco)


text: University of Maryland

text: live

text: Generic

text: Homo sapiens

characteristic: organism

text: Male and Female

characteristic: sex

text: Adipose

text: Human Fat Cell 5'-Stretch Plus cDNA Library

text: lambdaTriplEx


text: Universite Pierre et Marie Curie

text: live

text: Generic

text: RT-PCR using degenerate CYP4 primers. Cloning in PGEM-T easy

text: Adults

characteristic: dev-stage

text: Daphnia pulex

text: Daphnia pulex CYP4 from two ecotypes

text: Plasmid

text: PGEMT-easy

characteristic: vector


text: Oklahoma State University

text: live

text: Generic

text: embryonic fibroblast

text: Rat1 + ER alpha

characteristic: cell-line

text: Rattus norvegicus

text: Rat1 + ER alpha Suppression Subtractive Hybridization Library

characteristic: title


characteristic: External Id

text: MPI for Breeding Research

characteristic: INSDC center name

text: live

text: Generic

text: Solanum tuberosum

text: leaf

characteristic: tissue-type

text: Solanum tuberosum leaf


text: Universita' di Padova

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: larval

text: Ciona intestinalis

text: Stratagene UniZAP whole-larva library


text: Institute of Arable Crop Research

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: mercia

text: 28 days post anthesis

text: Triticum aestivum

text: embryo


text: Institute of Arable Crop Research

text: live

text: Generic

characteristic: NCBI submission package

text: mercia

text: 40 days post anthesis

text: Triticum aestivum

text: embryo


text: Institute of Arable Crop Research

text: live

text: Generic

text: mercia

characteristic: cultivar

text: 21 days post anthesis

text: Triticum aestivum

text: embryo


text: Institute of Arable Crop Research

text: live

text: Generic

text: mercia

text: 30 days post anthesis

text: Triticum aestivum

text: embryo


text: Institute of Arable Crop Research

text: live

text: Generic

text: mercia

text: 1 day post germination

text: Triticum aestivum

text: embryo


text: Institute of Arable Crop Research

text: live

text: Generic

text: mercia

text: 2 days post germination

text: Triticum aestivum

text: embryo


text: Institute of Arable Crop Research

text: live

text: Generic

text: mercia

text: 2 days post germination

text: Triticum aestivum

text: aleurone


text: Institute of Arable Crop Research

text: live

text: Generic

text: mercia

text: 21 days old

text: Triticum aestivum

text: cold stressed seedlings


text: Institute of Arable Crop Research

text: live

text: Generic

text: mercia

text: 21 days old

text: Triticum aestivum

text: drought stressed seedlings


text: Institute of Arable Crop Research

text: live

text: Generic

text: mercia

text: 21 days old

text: Triticum aestivum

text: salt stressed seedlings


text: Institute of Arable Crop Research

text: live

text: Generic

text: mercia

text: 14 days post anthesis

text: Triticum aestivum

text: embryo


text: Institute of Arable Crop Research

text: live

text: Generic

text: mercia

text: 21 days old

text: Triticum aestivum

text: waterlogged roots


text: Institute of Arable Crop Research

text: live

text: Generic

text: mercia

text: 21 days old

text: Triticum aestivum

text: normal roots


text: Institute of Arable Crop Research

text: live

text: Generic

text: mercia

text: 21 days old

text: Triticum aestivum

text: normal seedlings


text: Institute of Arable Crop Research

text: live

text: Generic

text: mercia

text: 21 days post germinaton

text: Triticum aestivum

text: nitrate starved seedlings


text: Institute of Arable Crop Research

text: live

text: Generic

text: florida

text: 2 days post fertilization

text: Triticum aestivum

text: ovules


text: Institute of Arable Crop Research

text: live

text: Generic

text: mercia

text: 21 days post germinaton

text: Triticum aestivum

text: nitrate starved roots


text: Institute of Arable Crop Research

text: live

text: Generic

text: florida

text: pre-fertilization

text: Triticum aestivum

text: ovules


text: Institute of Arable Crop Research

text: live

text: Generic

text: florida

text: pre-fertilisation

text: Triticum aestivum

text: egg cell


text: Flinders University

text: live

text: Generic

text: Bos taurus

text: retinal pigment epithelium

text: Bos taurus retinal pigment epithelium


text: Oklahoma State University

text: live

text: Generic

text: embryonic fibroblast

characteristic: cell type

text: Rat1 + ER beta

text: Rattus norvegicus

text: Rat1 + ER beta Suppression Subtractive Hybridization Library


text: Agriculture and Agri-food Canada

text: live

text: Generic

text: Westar

text: Germinated in soil flats and seedlings grown for 3 weeks in a Conviron E-15 cabinet set at 20oC /16 hr light (250 Em-2sec-1) and 16 oC / 8 hr dark. Fourth leaves collected at 9 am and immediately frozen.

characteristic: description

text: 3 weeks seedling grown at room temperature

text: Brassica napus

text: EcoRI

text: XhoI

text: fourth leaf

text: Bn01_AAFC_ECORC_transgenic_Brassica_napus_overexpressing_BNCBF17_constitutively_frost_tolerant

text: Plasmid

text: Bluescript SK+/XhoI-EcoRI


text: Institut fuer Genetik der Universitaet zu Koeln

text: live

text: Generic

text: adult - varying posteclosion times

text: Drosophila yakuba

characteristic: organism

text: both

text: wild tipe

characteristic: strain

text: Drosophila yakuba whole organism adult library

text: Uni-ZAP XR (Startagene)


text: live

text: Generic

text: embryo 0-14 h

characteristic: dev-stage

text: Drosophila yakuba

text: both

characteristic: sex

text: wild tipe

text: Drosophila yakuba 0-14 h embryo library

text: Uni-ZAP XR (Startagene)


text: MRC Human Genome Mapping Project Resource Centre

text: live

text: Generic

text: Takifugu rubripes

text: whole body

text: EFRa

text: phagemid

text: pBluescript II KS

characteristic: vector


text: live

text: Generic

text: Takifugu rubripes

text: spleen

text: EFRb

characteristic: title

text: phagemid

text: pBluescript II KS


characteristic: External Id

text: live

text: Generic

text: Takifugu rubripes

text: gill

text: EFRc

text: phagemid

text: pBluescript II KS


characteristic: INSDC center name

text: live

text: Generic

text: Takifugu rubripes

text: gut

text: EFRd

text: phagemid

text: pBluescript II KS


text: live

text: Generic

text: Takifugu rubripes

text: gonad

characteristic: tissue-type

text: EFRe

text: phagemid

text: pBluescript II KS


characteristic: INSDC first public

text: live

text: Generic

text: Takifugu rubripes

text: brain

text: EFRf

text: phagemid

text: pBluescript II KS


characteristic: INSDC last update

text: live

text: Generic

text: Takifugu rubripes

text: eye

text: EFRg

text: phagemid

text: pBluescript II KS


text: live

characteristic: INSDC status

text: Generic

text: Takifugu rubripes

text: liver

text: EFRh

text: phagemid

text: pBluescript II KS


text: live

text: Generic

characteristic: NCBI submission model

text: Takifugu rubripes

text: kidney

text: EFRj

text: phagemid

text: pBluescript II KS


text: live

text: Generic

characteristic: NCBI submission package

text: adult

text: Takifugu rubripes

text: female

text: skin

text: phagemid


text: live

text: Generic

text: adult

text: Takifugu rubripes

text: female

text: intestine

text: phagemid


text: live

text: Generic

text: adult

text: Takifugu rubripes

text: female

text: ovary

text: phagemid


text: live

text: Generic

text: adult

text: Takifugu rubripes

text: female

text: fin

text: phagemid


text: New York University School of Medicine

text: live

text: Generic

text: salivary gland sporozoite

text: Subtractive cDNA library salivary gland sporozoites versus oocyst sporozoites of the rodent malaria parasite Plasmodium berghei. The identified genes are asociated with an increase in virulence of salivary gland sporozoites.

text: Anopheles stephensi

characteristic: lab-host

text: Plasmodium berghei

text: Pb sgSpz/ooSpz subtraction library

text: Plasmid


text: Molecular Engines Laboratories

text: live

text: Generic

text: macrophage, histiocyte

characteristic: cell type

characteristic: cell-line

text: Homo sapiens

text: histiocytic lymphoma

text: differential display in Tumor Reversion


text: Xiangya Medical School of Central Southern University

text: live

text: Generic

text: embryo

text: Collecting 20-30 8-cell embryos and 8-cell compacted embryos respectively from Kunmingbai mouse and rinsed three times in 1-2ml of PBS solution. Remove any remaining cumulus cells. Treated with RNAlater solution and remove the zona pellucida using acid Tyrode's solution. Samples in 3-6 ul of lysis buffer were incubated for 5 min at 65oC water-bath to lyse the cells and release RNA. Synthesize cDNA from the 8-cell embryos and 8-cell compacted embryo by SMART-PCR respectively. Make the cDNA of 8-cell embryos as driver and cDNA of 8-cell compacted embryos as tester and preparing the subtracted library according to CLONTECH PCR-SelectTM cDNA Subtraction Kit User Manual.

characteristic: description

text: 8-cell compacted embryo

text: Mus musculus

characteristic: organism

text: KM

text: subtracted mouse compacted embryo cDNA library

text: Plasmid

text: pUCm-T


text: US Department of Agriculture, Agriculture Research Service, Pacific West Area, Western Regional Research Center

text: live

text: Generic

text: Chinese Spring

characteristic: cultivar

text: Hydroponic plants grown to full tillering stage were treated with 150 mM NaCl for either 12 hours or 7 days. Root tissues of the plants subjected to both types of treatment were collected separately at University of California, Davis (E. Akhunov and K. Deal in J. Dvorak's Lab). Total RNA was prepared separately from the two samples (12h and 7day treatments), and equal amount of RNA was then pooled. PolyA RNA was purified from the pooled RNA, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript SK(-) phagemids in J. Dvorak's lab (E. Akhunov, J. Dvorak) at the University of California, Davis. Colony plating, plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

text: Full tillering

characteristic: dev-stage

text: E. coli SOLR

text: Triticum aestivum

text: EcoRI

text: XhoI

text: Roots

text: Wheat salt-stressed root cDNA library

text: Phagemid

text: Lambda Uni-ZAP XR, excised phagemid pBluescript SK(-)


text: Washington University School of Medicine

text: live

text: Generic

text: cDNAs were synthesized from poly(A)+ RNA by oligo d(T) priming and directionally cloned into the Uni-ZAP XR lambda vector (Stratagene). The library was mass excised as phagemid, and DNA was purified by phenol extraction and alcohol precipitation. Single-strand phagemid DNA was transformed in mass into DH10B host cells. The library was plated and hybridized with a pool of 14 over-represented ESTs (N>30, from 4949 previous reads) and non-hybridizing colonies were selected for sequencing.

text: merozoite

text: Sarcocystis neurona

text: EcoRI

text: XhoI

text: cSn 1 3 S neurona subtracted cDNA

text: plasmid

text: pBluescript SK-

characteristic: vector


text: Universita' di Padova

text: live

text: Generic

text: country=Italy

text: larva

text: Ciona intestinalis

text: Ciona intestinalis larva

characteristic: title


characteristic: External Id

text: University of Minnesota

text: live

text: Generic

text: isolate: 2sp2 ; cDNA was prepared from polyA+ enriched RNA The cDNA was ligated into Lambda gt11 from Stratagene and packaged using Gigapack packaging extracts. An aliquot of the amplified library was used to transduce E. coli Y1090 and phage DNA was purified from a liquid lysate. The cDNA inserts were gel purified after EcoRI digestion and ligated into pBluescript SK+. Aliquots of the ligation were used to transform E. coli DH5alpha which were plated onto medium with X-gal for selection of recombinants.

text: Young, actively growing mycelia (3 days after inoculation) grown in liquid culture (cutin minimal medium containing 2%glucose).

text: Colletotrichum trifolii

text: EcoRI

text: EcoRI

text: race 1

characteristic: strain

text: mycelia

text: DSCT

text: plasmid

text: pBluescript SK+


text: First Affiliated Hospital of Guangxi Medical University

characteristic: INSDC center name

text: live

text: Generic

text: Epithelial

text: Adult

text: Prostate

characteristic: organ

text: Homo sapiens

text: male

characteristic: sex

text: prostate

characteristic: tissue-type

text: prostate epithelial different express Lib


characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: stromal

text: Adult

text: Prostate

text: Homo sapiens

text: male

text: prostate

text: prostate stromal different express Lib


text: Scottish Crop Research Institute

text: live

text: Generic

characteristic: NCBI submission package

text: Optic

text: Non-normalised library, directionally cloned into pSPORT1. Derived from roots of 3 week old drought stressed barley plants. After two weeks in control conditons the seedlings were raised above the hydroponic solution for 3 hours daily. N.B. This library has been superseded by EBro08. Developed as part of the barley transcriptome resources of BBSRC/SEERAD funded cereal IGF (Investigating Gene Function) project.

text: 3 week

characteristic: lab-host

text: Hordeum vulgare subsp. vulgare

text: Sal I

text: Not I

text: root

text: root, 3 week, drought-stressed, cv Optic, EBro06


text: live

text: Generic

text: NCI_CGAP_FH0 is a cDNA library containing the following tissue(s): Human Grade 1 Chondrosarcoma Cell Line The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is AGAATCCGGC. The cell line was provided by Dr James Martin from University of Iowa

characteristic: description

text: Adult

text: DH10B (Life Technologies)

text: Bone

text: Homo sapiens

characteristic: organism

text: EcoR I

text: Not I

text: Human Chondrosarcoma Cell Line

text: Plasmid

text: pT7T3-Pac (Pharmacia) with a modified polylinker


text: China Agricultural University

text: live

text: Generic

text: Triticum aestivum

text: Triticum aestivum photoperiod-temperature sensitive genic male sterile line


text: Lombardi Cancer Center

text: live

text: Generic

characteristic: cell-line

text: Homo sapiens

text: Homo sapiens PCI-O6A; head and neck squamous cell carcinoma


text: live

text: Generic

text: NCI_CGAP_FE0 is a cDNA library containing the following tissue(s): a pool of 3 chondrosarcoma cell lines ( grade 2) The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CGCTACGGAC.The cell lines was provided by Dr James Martin of University of Iowa.

text: Adult

characteristic: dev-stage

text: DH10B (Life Technologies)

text: Homo sapiens

text: EcoR I

text: Not I

text: Chondrosarcoma Cell line

text: Plasmid

characteristic: vector


characteristic: External Id

text: live

text: Generic

text: The library was constructed according Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. Denatured RNA was size fractionated on a 1% agarose gel.First strand cDNA synthesis was primed with oligo-dT primer containing a Not I site. Double strand cDNA was size selected according to mRNA size fraction, ligated with EcoR I adaptor, digested with NotI and then cloned directionally into pYX-Asc vector. The library tag sequence located between the Not I site and the polyA tail is CAGCCACGAC. This library was created for the University Iowa Brain Anatomy Project (BMAP): 'Gene Discovery in the Developing Mouse Nervous System', supported by National Institute of Mental Health (NIMH), Hemin Chin, Ph.D., program coordinator.

text: embryo 12.5dpc

text: DH10B (T1 phage resistant)

text: Brain

text: Mus musculus

text: EcoR I

text: Not I

text: whole brain

characteristic: title

text: Plasmid

text: pYX- Asc


text: Children's Memorial Research Center

characteristic: INSDC center name

text: live

text: Generic

text: UI-R-FF0 is a subtracted cDNA library containing the following tissue(s): Normal cartilage and SR-JWS Tumor Line . The subtraction was made according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tags for these libraries are: CTAATGGACG, CATTCTTGTA.

text: Adult

text: DH10B (Life Technologies) (T1 phage resistant)

text: Rattus norvegicus

text: EcoR I

text: Not I

text: Mixed tissues

text: Plasmid


text: Oklahoma State University

text: live

text: Generic

text: embryonic fibroblast

characteristic: cell type

text: Rat1 + ER alpha

text: Rattus norvegicus

text: Rat1 + ER alpha vehicle Suppression Subtractive Hybridization Library


text: College of Life Sciences

text: live

text: Generic

text: Rice seedlings were infested by brown planthopper. Seedling samples were collected 36 hour after infesting and mRNAs were used for construction of differentially expressed cDNA libraries by suppression subtractive hybridization

text: Two-week-old rice seeding infested by brown planthopper

text: Oryza sativa

text: B5 resistant variety to brown planthopper

characteristic: strain

text: Differentially expressed cDNA libraries of B5 resistant variety rice seedlings responding to brown planthopper feeding

text: pT-Adv


text: University of Wisconsin-Madison

text: live

text: Generic

text: Subtracted cDNAs of early 4th instars from early pupal cDNAs were cloned. The 4th instar subtracted cDNA library was enriched for genes that express at relatively higher levels between 12 to 24 hours post 3rd molt.

text: 4th stadium and early pupae

text: Aedes aegypti

text: Mixed

characteristic: sex

text: Rockefeller

text: 4th instar subtracted cDNA library

text: Plasmid

text: pT-Adv


text: Purdue University

characteristic: INSDC first public

text: live

text: Generic

text: egg, larvae, worker, presoldier, soldier, nymph, queen, king

text: Reticulitermes flavipes

text: male and female

text: 'EFOB'

text: whole organism

characteristic: tissue-type

text: R. flavipes 'EFOB' colony

text: Uni-ZAP XR


text: Institute of Genetics and Developmental Biology, CAS

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: The leaves of Wheat-Thinopyrum alien additional line TAI-27 at three leaf stage were infected by aphid carrying BYDV (Barley Yellow Dwarf Virus). Then the subtracted library was constructed using infected leaves and the control.

text: three-leafed stage

text: Triticum aestivum/Thinopyrum intermedium alien addition line

text: PstI

text: PstI

text: leaf

text: Wheat-Thinopyrum alien additional line TAI-27 subtracted cDNA lib

text: PUCmT-Vector


text: US Department of Agriculture, Agriculture Research Service, Pacific West Area, Western Regional Research Center

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Chinese Spring

characteristic: cultivar

text: Plant tissues from wheat cv. CS grown to full tillering stage in greenhouse were collected at University of California, Davis (Jan Dvorak's lab, K. Deal and E. Akhunov). Total RNA was prepared from leaves (young leaf and third leaf), whole roots, crown, steam and sheath tissues, and then equal quantities of RNA were pooled from the these samples. PolyA was purified from the pooled RNA, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript SK(-) phagemids in J. Dvorak's lab (E. Akhunov, J. Dvorak) at the University of California, Davis. Colony plating, plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

text: Adult

text: E. coli SOLR

text: Triticum aestivum

text: EcoRI

text: XhoI

text: Roots, leaves, crown, stem and sheath

text: Wheat CS whole plant cDNA library

text: Phagemid

text: Lambda Uni-ZAP XR, excised phagemid pBluescript SK(-)


text: University of Wisconsin-Madison

text: live

text: Generic

text: Subtracted cDNAs of early pupae from early 4th instars were cloned. The early pupal subtracted cDNA library contained more genes that were transcribed between 12 to 24 hours post pupation.

characteristic: description

text: Early pupae

text: Aedes aegypti

characteristic: organism

text: Mixed

text: Rockefeller

text: Early pupa subtracted cDNA library

text: Plasmid

text: pT-Adv


text: Texas A&M University

text: live

text: Generic

text: 5' end of cDNA cloned into EcoRI site; 3' end of cDNA cloned into XhoI site

text: Fusarium sporotrichioides

text: EcoRI

text: XhoI

text: Fusarium sporotrichioides Tri10 overexpressed cDNA library

text: pBlueScript SK-


text: Universite de Bordeaux I, Institut National de la Recherche Agronomique

text: live

text: Generic

text: Cabernet Sauvignon

text: Oriented library

text: veraison stage

characteristic: dev-stage

text: seeds

characteristic: organ

text: Vitis vinifera

text: SfiIA

text: SfiIB

text: Grape berries seeds Lambda TriplEx2 Library

text: Phage

text: Lambda TriplEx2


characteristic: External Id

text: UMR CNRS 6161, Universite de Poitiers

text: live

text: Generic

text: Ugni blanc

text: Oriented library

text: Veraison stage

text: Fruit

text: Vitis vinifera

text: SalI

text: NotI

text: Veraison Grape berries SuperScriptTM Plasmid Library

text: plasmid

characteristic: vector


text: Institut National de la Recherche Agronomique

characteristic: INSDC center name

text: live

text: Generic

text: Shiraz

text: Oriented library, construction described in Generation of ESTs from grape Berry (skin, pulp or seeds) at various developmental stages by Terrier,N., Ageorges,A., Abbal,P., Romieu,C. in J. Plant Physiol. 158 (12): 1575-83 2001

text: veraison stage

text: Fruit

text: Vitis vinifera

text: Eco RI

text: XhoI

text: Veraison Grape berries Lambda Zap II Library

characteristic: title

text: Phage

text: Lambda Zap II


text: Universidade Federal de Sao Paulo, UNIFESP/EPM

text: live

text: Generic

text: non-normalized cDNA library

text: epimastigote

text: Trypanosoma cruzi

text: CL Brener

characteristic: strain

text: Trypanosoma cruzi CL Brener epimastigote


text: live

text: Generic

text: One-month old plants were subjected to vernalization treatment by placing them in the cold room at 6 C, under 15hr light/9hr dark condition. Total RNA was prepared from apex tissue extracted from plants with no cold treatment; and from plants with 2-week, 4-week and 6-week cold treatment separately. Equal amount of total RNA was pooled from all four samples, a cDNA library was made using pooled polyA RNA and cDNA clones were in vivo excised at the University of California, Davis (V. Echenique, B. Stamova, J. Dubcovsky). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

text: One month old plants

text: E. coli XLOLR

characteristic: lab-host

text: Triticum monococcum

text: EcoRI

text: XhoI

text: Vernalized apex

text: Triticum monococcum vernalized apex cDNA library

text: Phagemid

text: Lambda Uni-ZAP XR, excised phagemid


text: The Sanger Centre

characteristic: INSDC first public

text: live

text: Generic

text: Homo sapiens


text: The Sanger Centre

text: live

text: Generic

text: Homo sapiens

text: Homo sapiens library (Ashcroft K)


text: MRC Human Genome Mapping Project Resource Centre

text: live

text: Generic

text: adult

text: Takifugu rubripes

text: female

characteristic: sex

text: heart

characteristic: tissue-type

text: phagemid


characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: adult

text: Takifugu rubripes

text: female

text: muscle

text: phagemid


text: Miyazaki University

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: common name:common carp ; stimulated with peptidoglycan

text: Cyprinus carpio

text: head kidney

text: Cyprinus carpio head kidney stimulated with peptidoglycan


text: INSERM U433 , Faculte Medecine Laennec

text: live

text: Generic

text: adult

text: Mus musculus

text: female

text: Swiss

text: hypothalamus

text: Mus musculus hypothalamus Swiss adult


text: MPI for Breeding Research

text: live

text: Generic

text: Provita

characteristic: cultivar

text: Solanum tuberosum

text: tuber

text: Solanum tuberosum tuber


text: MPI for Breeding Research

text: live

text: Generic

text: Provita

text: Solanum tuberosum

characteristic: organism

text: Solanum tuberosum cv. Provita


text: MPI for Breeding Research

text: live

text: Generic

text: Solanum tuberosum

text: Solanum tuberosum library (Gebhardt C)


text: MPI for Breeding Research

text: live

text: Generic

text: Saturna

text: Solanum tuberosum

text: Solanum tuberosum cv. Saturna


text: James A. Baker Institute, Cornell University

text: live

text: Generic

text: The library was prepared and supplied by Ms. Nancy MacAfee and Dr. Michael Burt at the University of New Brunswick, New Brunswick, Canada.

characteristic: description

text: adult

characteristic: dev-stage

text: Parelaphostrongylus tenuis

text: mixed

text: Adult Parelaphostrongylus tenuis cDNA library

text: Phage

text: Uni-ZAP XR vector


characteristic: External Id

text: US Department of Agriculture, Agriculture Research Service, Pacific West Area, Western Regional Research Center

text: live

text: Generic

text: Butte 86

text: Plants were grown under six following different environmental regimes in greenhouse, Environment 1) 24oC/17oC day/night, well-watered, with post-anthesis fertilizer, Environment 2) 24oC/17oC day/night, well-watered, without post-anthesis fertilizer, Environment 3) 37oC/17oC day/night, well-watered, with post-anthesis fertilizer, Environment 4) 37oC/17oC day/night, well-watered, without post-anthesis fertilizer, Environment 5) 37oC/17oC day/night plus drought, with post-anthesis fertilizer, Environment 6) 37oC/17oC day/night plus drought, without post-anthesis fertilizer, developing wheat grains from the following were excised and frozen in liquid nitrogen, Environment 1 at 3, 5, 7, 8, 10, 12, 16, 20, 24, 28, 32, 36, 40, 44 DPA Environment 2 at 3, 5, 7, 8, 10, 12, 16, 20, 24, 28, 32, 36, 40, 44 DPA Environment 3 at 3, 5, 7, 8, 10, 12, 16, 20, 24, 28, 32, 34 DPA Environment 4 at 3, 5, 7, 8, 10, 12, 16, 20, 24, 28, 32, 34 DPA Environment 5 at 3, 5, 7, 8, 10, 12, 16, 20, 24, 28, 30 DPA Environment 6 at 3, 5, 7, 8, 10, 12, 16, 20, 24, 28, 30 DPA and total RNA was prepared by S. Altenbach and K. Cronin at USDA-ARS, Albany, CA. A cDNA library was made using poly (A) RNA, and the cDNA clones were in vivo excised to give pBluescript SK(-) phagemids in the TJ Close lab (Chin, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (others).

text: 3-44 days post anthesis seed

text: E. coli SOLR

text: Triticum aestivum

text: EcoRI

text: whole grains

text: Wheat developing grains cDNA library

text: Phagemid

text: Lambda ZAP II, excised phagemid


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 2.2 kb. Constructed by Invitrogen. Note: this is a NCI_CGAP Library.

text: DH10B (phage-resistant)

text: brain

characteristic: organ

text: Macaca mulatta

text: NotI

text: EcoRV

text: hypothalamus

text: phagemid (ampicillin resistant)

characteristic: vector


text: University of Stirling

characteristic: INSDC center name

text: live

text: Generic

text: country=United Kingdom

text: adult

text: Salmo salar

text: kidney

text: Salmo salar kidney adult Uni-Zap

characteristic: title

text: Stratagene lambda Uni-Zap


text: University of Stirling

text: live

text: Generic

text: country=United Kingdom

text: adult

text: Salmo salar

text: kidney

text: Salmo salar kidney adult pT-Adv

text: Clontech pT-Adv


text: University of Stirling

text: live

text: Generic

text: country=United Kingdom

text: adult

text: Salmo salar

text: spleen

text: Salmo salar spleen adult

text: Stratagene lambda Uni-Zap


text: Tel-Aviv University

text: live

text: Generic

text: Colletotrichum aeschynomenes

text: Colletotrichum gloeosporioides f. sp. aeschynomene library


text: Duke University

text: live

text: Generic

text: Deflagellation library, constructed by John Davies and Jeffrey McDermott, combines cDNAs from CC-1690 cells which had been re-synthesizing flagella for 15, 30 and 60 min after being deflagellated by pH shock. PolyA mRNA was purified from each sample, pooled and cDNA synthesized. The cDNA was directionally cloned into lambda Zap II (Stratagene) in the EcoRI (5') and XhoRI (3') sites. pBluescript II SK- plasmids were excised from the lambda ZAP clones by superinfection with ExAssist (Stratagene) phage. The library was normalized using method 4 described in Bonaldo et al., (1996) Genome Research 6: 791-806.

text: Chlamydomonas reinhardtii

text: EcoRI

text: XhoI

text: CC-1690 wild type mt+ 21gr

characteristic: strain

text: C. reinhardtii CC-1690, Deflagellation (normalized), Lambda Zap II

text: plasmid

text: pBluescript II SK-


characteristic: INSDC first public

text: live

text: Generic

text: colon

text: Homo sapiens

text: colon adenoma

text: Human colon adenoma cDNA library


text: Institute of Himalayan Bioresource Technology

text: live

text: Generic

text: T 78

text: Differential display mediated generation of 3' ESTs was performed as detailed by Liang et al. (Nucleic Acids Research 22, 5763-5764). Identified Ests were cloned into pCRTRAP vector at HindIII site.

text: 14 days of stress and ABA treatment and 4 days of recovery

text: E. coli

characteristic: lab-host

text: LEAF

text: Camellia sinensis

text: HindIII

text: hermaphrodite

characteristic: sex

text: Whole leaf tissue

text: Camellia sinensis Differential display

text: Plasmid

text: pCRTRAP


text: The Institute of Crop Germplasm Resources

characteristic: INSDC last update

text: live

text: Generic

text: Barley leaves treated 24hrs with 430mM NaCl

text: Hordeum vulgare subsp. spontaneum

text: leaf

characteristic: tissue-type

text: Barley leaf cDNA library


text: Oklahoma State University

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Jackpot

text: Clontech PCR-select cDNA subtraction library

text: E.coli

text: Cynodon dactylon

text: EcoR I

text: EcoR I

text: crown

text: Bermudagrass cv. Jackpot SSH Library

text: plasmid

text: pCR 2.1-TOPO


text: Oklahoma State University

text: live

text: Generic

characteristic: NCBI submission package

text: Yukon

characteristic: cultivar

text: E.coli

text: Cynodon dactylon

text: EcoR I

text: EcoR I

text: crown

text: Bermudagrass cv. Yukon SSH Library

text: plasmid

text: pCR 2.1-TOPO


text: University of Bonn

text: live

text: Generic

text: leukocytes

characteristic: cell type

text: cDNA fragments obtained from differential display RT-PCR banding patterns were cloned into pGEM

characteristic: description

text: Sus scrofa

characteristic: organism

text: differential display RT-PCR clones from Sus scrofa leukocytes

text: pGEM


characteristic: External Id

text: University of Bonn

text: live

text: Generic

text: leukocytes

text: cDNA fragments obtained from cDNA-AFLP banding patterns were cloned into pGEM

text: Sus scrofa

text: cDNA-AFLP clones from Sus scrofa leukocytes

text: pGEM


text: CNRS, INSERM, ULP

text: live

text: Generic

text: Oligo dT cDNA library constructed from RNA of 26 somite stage zebrafish embryos

text: 26 somite embryos

characteristic: dev-stage

text: Danio rerio

text: NotI

text: SalI

text: 26 somite embryos

text: pSPORT1 (BRL)

characteristic: vector


text: Faculty of Agriculture and Natural Science, University of Pretoria

characteristic: INSDC center name

text: live

text: Generic

text: PI 294994

text: mRNA/cDNA

text: 5 days post Russian wheat aphid infestation

text: Triticum aestivum

text: leaf

text: Wheat PI294994 RWA induced cDNA library

characteristic: title


text: Washington University School of Medicine

characteristic: INSDC first public

text: live

text: Generic

text: 7-day-old protonemal tissue was incubated 10-4M ABA for 16 hours before RNA isolation. cDNA was synthesised from Amersham's cDNA Sythesis Plus kit and ligated with EcoRI-NotI linkers for cloning in the EcoRI site of Lampda ZapII (Stratagene). After packaging, the library was propogated in E. coli XL-I Blue cells and amplified. The library was excised by mass excision in SOLR cells and ampicillan resistant transformants selected. Approximately, 1,000,000 colonies were grown and recovered. The double stranded plasmid library was recovered and used to transform DH10b cells by electroporation. Clones corresponding to abundant transcripts were identified by colony hybridization using a cDNA probe derived from untreated protonematal tissue, and eliminated from the library, by rearraying.

text: protonemata, 7day old ABA-treated

text: E.coli DH10b

text: Physcomitrium patens

text: EcoRI

text: EcoRI

text: Moss EST library PPAS

text: plasmid

text: pBluescript SK-


text: University of Edinburgh

text: live

text: Generic

text: Ascaris lumbricoides 'large roundworm' is a nematode parasite of humans. The cDNA was obtained from male adult worms whole body. cDNA was directionally cloned into a phagemid (pBLUESCRIPT) vector. cDNA was ligated to EcoRI adapters, digested with XhoI, and cloned into pBLUESCRIPT II SK (+) XR cut with EcoRI and XhoI.

text: adult

text: Ascaris lumbricoides

text: EcoRI

text: XhoI

text: Male

text: Ascaris lumbricoides adult male whole body

text: Phagemid

text: pBLUESCRIPTII SK (+) XR


text: LG Molekulargenetik

text: live

text: Generic

text: genotype A17; cDNA was prepared from total RNA using the SMART PCR cDNA system (Clontech) from roots harvested three weeks after inoculation with Glomus intraradices. This cDNA was used as tester in a Suppression Subtractive Hybridization (SSH). The SSH-cDNA fragments were generated using the SSH-adaptor sequences ctaatacgactcactatagggctcgagcggccgcccgggcaggt and ctaatacgactcactatagggcagcgtggtcgcggccgaggt (Clontech) and ligated after Suppression Subtractive Hybridization in to the pGEM-Teasy vector from Promega. Plasmids containing cDNA inserts were propagated in E. coli TOP 10F' cells (Invitrogen)

text: 3 weeks after inoculation

text: Medicago truncatula

text: mycorrhizal roots

text: MTGIM

text: pGEM-Teasy


text: Universitaet Bielefeld

text: live

text: Generic

text: genotype A17; cDNA was prepared from polyA+ enriched RNA from flowers harvested at different stages of development. The cDNA was directionally ligated into the pGEM-T vector from Promega. Plasmids containing cDNA inserts were propagated in E. coli DH5alpha cells.

text: different stages of development

text: Medicago truncatula

text: T-overhang

text: XhoI

text: flowers

text: MTFLOW

text: pGEM-T


text: Universitaet Bielefeld

text: live

text: Generic

text: genotype A17; cDNA was prepared from polyA+ enriched RNA from developing pods including seeds harvested at different stages of development. The cDNA was directionally ligated by MediGenomix into the pGEM-T vector from Promega using GCATGCGGCCGAGGCGGCCGACATG and CTGCAGGCCATTATGGCCGGG adapters. Plasmids containing cDNA inserts were propagated in E. coli DH10B cells.

text: different stages of development

text: Medicago truncatula

text: PstI

text: SphI

text: pods including seeds

text: MTPOSE

text: pGEM-T


text: Universitaet Bielefeld

text: live

text: Generic

text: genotype A17; cDNA was prepared from polyA+ enriched RNA from mycorrhizal roots harvested five weeks after inoculation. The cDNA was directionally ligated by MediGenomix into the pGEM-T vector from Promega using GCATGCGGCCGAGGCGGCCGACATG and CTGCAGGCCATTATGGCCGGG adapters. Plasmids containing cDNA inserts were propagated in E. coli DH10B cells.

text: six week old mycorrhizal roots harvested five weeks after inoculation with Glomus intraradices

text: Medicago truncatula

text: PstI

text: SphI

text: mycorrhizal roots

text: MTAMP

text: pGEM-T


text: Inst. de Ciencia Aplicada e Tecnologia, Ed. ICAT

text: live

text: Generic

text: Rocha

text: country=Portugal

text: Pyrus communis

text: fruit mesocarp

text: Pyrus communis fruit mesocarp


text: North Carolina State University

text: live

text: Generic

text: Karhumaki

text: Created using PCR Library Construction kit (Stratagene)

text: Arabidopsis lyrata

text: Inflorescence

text: Arabidopsis lyrata Inflorescence pCMV-PCR Library

text: Plasmid

text: pCMV-PCR (Stratagene)


text: University of Edinburgh

text: live

text: Generic

text: Psoroptes ovis is the infective agent causing sheep scab, a serious dermatitis in sheep. The cDNA was obtained from mites harvested from the skin of previously unexposed sheep harbouring a moderate challenge infection. The cDNA was cloned using the Clontech Lambda TriplEx2 Smart cDNA cloning system.

text: Mixed (predominantly adult)

text: Psoroptes ovis

text: Mixed

text: Psoroptes ovis mixed

text: Phagemid

text: Lambda TriplEX2


text: live

text: Generic

text: The library was constructed by Claire Murphy and Dr. James McCarter at Washington University, St. Louis. The cDNA was made by using Dynabead oligo-dT priming (Dynal). PCR based library using a modified protocol from the SMART PCR cDNA Synthesis Kit from Clontech. Directionally cloned into the UDG sites of pAMP1. 30-60 cell embryo RNA was provided by Dr. Richard Davis of City University of New York Graduate Center, College of Staten Island, Staten Island, NY (redavis@postbox.csi.cuny.edu).

text: 30-60 cell embryo

characteristic: lab-host

text: Ascaris suum

text: NotI

text: SalI

text: Ascaris suum embryo pAMP1 v1

text: plasmid (ampicillin resistant)

text: pAMP1 (Gibco)


text: live

text: Generic

text: The library was constructed by Claire Murphy and Dr. James McCarter at Washington University, St. Louis. The cDNA was made by using Dynabead oligo-dT priming (Dynal). PCR based library using a modified protocol from the SMART PCR cDNA Synthesis Kit from Clontech. Directionally cloned into the UDG sites of pAMP1. J2 were provided by Dr. Valerie Williamson of the University of California at Davis (vmwilliamson@ucdavis.edu).

text: Meloidogyne hapla

text: NotI

text: SalI

text: Meloidogyne hapla J2 pAMP1 v1

text: plasmid (ampicillin resistant)

text: pAMP1 (Gibco)


text: National Institute of Genetics

text: live

text: Generic

text: fry stage 40

text: Oryzias latipes

text: mixture of female and male

characteristic: sex

text: d-rR

characteristic: strain

text: whole embryo

text: MF01FSA cDNA


text: Institute for Animal Health

characteristic: INSDC last update

text: live

text: Generic

text: RNA was prepared from the thyroids of OS birds, which go on to suffer from spontaneous autoimmune thyroiditis, and those of control CB strain birds, using TRI Reagent. This was then enriched for polyadenylated RNA molecules using the Oligotex mRNA purification system (Qiagen), and used for SSH. Hybridisation was performed using the PCR-Select cDNA subtraction kit and the Advantage cDNA Polymerase Mix (both Clontech). Forward and reverse subtractions were performed.

text: Gallus gallus

text: male and female

text: Obese Strain (OS)

text: thyroid

characteristic: tissue-type

text: Chicken thyroid SSH library


text: National Institute of Genetics

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: segmentation stage 20 - 25

text: Oryzias latipes

text: mixture of female and male

text: Hd-rR

text: whole embryo

text: MF01SSB cDNA


text: live

text: Generic

characteristic: NCBI submission package

text: cDNA oligo dT-primed [5'-(GA)10-ACTAGTCTCGAGTTTTTTTTTTTTT-3'] and directionally cloned using 5' linkers 5'-AATTCGGCACGAG-3' and 5'-CTCGTGCCG-3'. Size selection of >400bp material gives average insert size ranging from 1-2 kb. Library was mass excised (from lambda-UniZAP-XR) and resulting single-stranded phagemids were prepped and tranformed into DH10B. Library constructed and donated by J. McCarrey, Ph.D. (Southwest Foundation for Biomedical Research, Dept. of Genetics); excision done by E.M. Eddy, Ph.D. (National Institutes of Health, National Institute of Environmental Health Sciences).

text: DH10B (phage-resistant)

text: testis

characteristic: organ

text: Mus musculus

text: XhoII

text: EcoRI

text: male

text: 18-day preleptotene spermatocytes

text: McCarrey Eddy 18 day preleptotene spermatocytes

text: phagemid (ampicillin resistant)

text: pBluescript SK+ (Stratagene)


text: US Department of Agriculture, Agriculture Research Service, Pacific West Area, Western Regional Research Center

text: live

text: Generic

text: F2 from 2-12-4-8-1-1-1-(1) x PI36909-12-811-(1)

characteristic: cultivar

text: Plants were grown in a growth chamber at the University of California, Davis (Akhunov in Devorak's lab). Pre-meiotic anthers were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids at the University of California, Davis (Akhunov in Devorak's lab). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

characteristic: description

text: Pre-meiotic anthers

text: E. coli SOLR

text: Aegilops speltoides

text: EcoRI

text: XhoI

text: Anther

text: Aegilops speltoides pre-meiotic anther cDNA library

text: Phagemid

text: Lambda Uni-ZAP XR, excised phagemid


characteristic: External Id

text: Tokyo University of Marine Science and Technology, Graduate School of Marine Science and Technology

text: live

text: Generic

text: skin

characteristic: cell type

text: Paralichthys olivaceus

characteristic: organism

text: flounder skin cells lambda ZAP II

text: lambda ZAP II


text: Purdue University

characteristic: INSDC center name

text: live

text: Generic

characteristic: cell-line

text: Subtracted libraries were prepared by 'suppressive subtractive hybridization' (SSH) approach using the Clontech PCR-selectTM cDNA Subtraction Kit (Clontech Laboratories Inc., Palo Alto, CA). SSH was performed using mRNA isolated from uninfected BY-2 cell suspension cultures and cultures infected for 12 h with the Agrobacterium strain At804. For 'forward' subtraction, RNA isolated from BY-2 cell suspension cultures infected A. tumefaciens At804 was used as the 'tester'; for 'reverse' subtraction it was used as the 'driver'. Final PCR products were cloned into the TA cloning vector pCR'2.1-TOPO (Invitrogen) and used to transform electro-competent E. coli DH10B (GIBCO-BRL).

text: Uninfected BY-2 cultures and cultures infected with Agrobacterium tumefaciens strain At804 for 12 h.

characteristic: dev-stage

text: Nicotiana tabacum

text: Cell suspension culture

text: Subtracted cDNA library from tobacco BY-2 infected with Agrobacterium tumifaciens


text: Max-Planck-Institut fur Zuchtungsforschung,

characteristic: INSDC first public

text: live

text: Generic

text: Dried leaves were used for cDNA preparation. Clones hybridising to RNA from dried leaves were selected.

text: non mature leaves

text: E.coli

characteristic: lab-host

text: Craterostigma plantagineum

text: PstI

text: PstI

text: dried leaves

characteristic: title

characteristic: vector


characteristic: INSDC last update

text: live

text: Generic

text: Substraction library. Untreated and ABA (mixed isomers, Sigma, Sigma-Aldrich, St. Louis, MO, USA)treated or ABA analog (kindly provided by Dr Suzanne Abrams, Plant Biotechnology Institute, National Research Council of Canada, Saskatoon, Canada) treated callus tissue were used. Identification of ABA inducible transcripts was carried out by selective amplification of differentially expressed sequences using the Clontech PCR-Select cDNA subtraction kit (Clontech Laboratories Inc., Palo Alto, CA, USA).

text: callus, 4d ABA treated

text: E.coli DH10B

text: Craterostigma plantagineum

text: EcoRI

text: EcoRI

text: ABA treated callus

text: pCR Script SK-


text: live

characteristic: INSDC status

text: Generic

text: Callus tissue was incubated ABA 10-4M for 2 day before RNA isolation. cDNA was synthesised from Super Script cDNA Synthesis Kit (Gibco BRL, Eggenstein, Germany) and ligated with the SalI and NotI restriction sites of the one-hybrid vector pPC86 (Chevray and Nathans, 1992). The resulting one-hybrid cDNA library was electroporated into competent DH10B E. coli cells (Gibco BRL, Eggenstein, Germany). After amplification in selective medium, plasmid DNA was prepared from the cDNA clones and selected at random for sequencing.

text: callus, 2d ABA-treated

text: E.coli DH10B

text: Craterostigma plantagineum

text: SalI

text: NotI

text: 2d-old ABA treated callus


text: University of California at Davis (UCD)

text: live

text: Generic

characteristic: NCBI submission model

text: Salinas

text: The library was constructed from 10 different sources of RNA from a single genotype. Separate cDNAs were generated using primers that incorporated unique 5' and 3' tags to distinguish each source of RNA. cDNAs were then pooled, size-fractionated, directionally cloned into a custom medium-copy vector and transformations made with four size classes to minimize size bias. Details of each source of RNA and library construction can be obtained at http://cgpdb.ucdavis.edu/

text: E.coli

text: Lactuca sativa

text: QG_ABCDI lettuce salinas

text: Plasmid

text: pBRcDNASfiAB


text: University of California at Davis (UCD)

text: live

text: Generic

characteristic: NCBI submission package

text: E.coli

text: Lactuca serriola

text: QG_EFGHJ lettuce serriola

text: Plasmid

text: pBRcDNASfiAB


text: University of California at Davis (UCD)

text: live

text: Generic

text: The library was constructed from 11 different sources of RNA from a single genotype. Separate cDNAs were generated using primers that incorporated unique 5' and 3' tags to distinguish each source of RNA. cDNAs were then pooled, size-fractionated, directionally cloned into a custom medium-copy vector and transformations made with four size classes to minimize size bias. Details of each source of RNA and library construction can be obtained at http://cgpdb.ucdavis.edu/

text: E.coli

text: Helianthus annuus

text: QH_ABCDI sunflower RHA801

text: Plasmid

text: pBRcDNASfiAB


text: University of California at Davis (UCD)

text: live

text: Generic

text: E.coli

text: Helianthus annuus

text: QH_EFGHJ sunflower RHA280

text: Plasmid

text: pBRcDNASfiAB


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.95 kb. Constructed by ResGen, Invitrogen Corp. Note: this is a NIH_MGC Library.

text: DH10B (phage-resistant)

text: otocysts

characteristic: organ

text: Mus musculus

text: EcoRV

text: NotI

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.7 kb. Constructed by ResGen, Invitrogen Corp. Note: this is a NIH_MGC Library.

text: DH10B (phage-resistant)

text: Mus musculus

text: EcoRV

text: NotI

text: undifferentiated limb

characteristic: tissue-type

text: phagemid (ampicillin resistant)


text: Sichuan University

text: live

text: Generic

text: Using rat aorta of hypercoagulable state as th tester and the normal rat aorta as the driver, suppression subtractive hybridization(SSH) was performed. Then I cloned the cDNA fragments into the pMD18-T vector, and I got a library of about 1000 clones. Part of the clones were sequenced.

characteristic: description

text: hypercoagulable State

text: Rattus norvegicus

text: EcoRI

text: HindIII

text: Male

characteristic: sex

text: Wistar

characteristic: strain

text: aorta

text: Hypercoagulable rat aorta subtracted cDNA library

text: plasmid


characteristic: External Id

text: Agriculture and Agri-food Canada

text: live

text: Generic

characteristic: cultivar

text: Controled chamber-grown wheat heads were spray inoculated at mid-anthesis with a Fusarium graminearum macroconidial suspension (50,000 spores/ml) and kept under intermitent misting for 24 hours, then collected and immediately frozen in liquid nitrogen.

text: anthesis

text: Triticum aestivum

characteristic: organism

text: EcoRI

text: EcoRI

text: heads

text: Ta02_AAFC_ECORC_Fusarium_graminearum_inoculated_wheat_heads

text: Plasmid

text: pGEM-T easy


text: Agriculture and Agri-food Canada

characteristic: INSDC center name

text: live

text: Generic

text: anthesis

characteristic: dev-stage

text: Triticum aestivum

text: EcoRI

text: EcoRI

text: heads

text: Ta03_AAFC_ECORC_Fusarium_graminearum_inoculated_wheat_heads

text: Plasmid

text: pGEM-T easy


text: Agriculture and Agri-food Canada

characteristic: INSDC first public

text: live

text: Generic

text: Harus

text: anthesis

text: Triticum aestivum

text: EcoRI

text: EcoRI

text: heads

text: Ta04_AAFC_ECORC_Fusarium_graminearum_inoculated_wheat_heads

text: Plasmid

text: pGEM-T easy


text: Stanford University

text: live

text: Generic

text: This library represents the unique genes found in the third round of EST sequencing at Stanford University for the maize genome project. Sequences are present from library 952. Contigs were assembled using ZmDBAssembler and 2 representatives from each contig were selected for the Unigene set. All singlets were also selected.

text: Zea mays

text: 1111 - Unigene III from Maize Genome Project

characteristic: title


text: Colgate University

text: live

text: Generic

text: authority=Fucus distichus (L.) Powell; EcoRI/XhoI fragments from Fucus _ZapII random-primed Fucus cDNA library were subcloned into EcoRI/XhoI sites of pSUC2T7M13ORI (Jacobs et al. 1997. Gene 198: 289).

text: 18h after fertilization

text: Fucus distichus

text: EcoRI

text: XhoI

text: Fucus 18h embryo SST secretion library

text: Plasmid

text: pSUC2T7M13ORI SST

characteristic: vector


text: Department of Genetics, The University of Melbourne

characteristic: INSDC last update

text: live

text: Generic

text: Total RNA was prepared from midguts of mid-fifth instar larvae of Helicoverpa armigera using the RNAgents kit (Promega). PolyA mRNA was obtained using the Dynabeads mRNA purification kit (Dynal). First-strand cDNA was made by oligo dT-priming with 5'-(GA)10-ACTAGTCTCGAGTTTTTTTTTTTTT-3'. Following second-strand cDNA synthesis, size-selected products >600 bp were ligated to 5' linkers 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3', directionally cloned into Lambda Zap II (Stratagene) in the EcoRI (5') and XhoI (3') sites, and packaged using Gigapack III Gold Packaging Extracts. The library was amplified, and plasmids were mass-excised using ExAssist helper phage (Stratagene) and propagated in XL1-Blue MRF' cells. Single-pass 5' end sequencing was performed using the T3 primer. Library construction supported by CESAR, a Special Research Centre of the Australian Research Council. Sequencing supported by the Director's Discretionary Fund of the Australian Genome Research Facility.

text: Fifth instar larvae

text: XL1-Blue MRF'

characteristic: lab-host

text: Helicoverpa armigera

text: Midgut

text: Helicoverpa armigera larval midgut cDNA library

text: Phagemid

text: pBluescript II SK(-)


text: University of Notre Dame

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: pupae, larvae, and adult

text: Anopheles funestus

text: Mali, field collected

text: SMART cDNA Anopheles Funestus Library


text: Clemson University

text: live

text: Generic

characteristic: NCBI submission package

text: Loring

text: authority=Prunus persica L. Batsh; The sequence has been trimmed to remove vector sequence and contains a minimum of 100 bases of phred value 20 or above. For more details on library preparation and sequence analysis go to http://www.genome.clemson.edu/projects/peach. To order this clone go to http://www.genome.clemson.edu/orders

text: E. coli

text: Prunus persica

text: EcoRI

text: XhoI

text: Mesocarp

text: Peach developing fruit mesocarp

text: Plasmid

text: pBluescript II SK(-)


text: live

text: Generic

text: The library was constructed according Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. Denatured RNA was size fractionated on a 1% agarose gel. First strand cDNA synthesis was primed with oligo-dT primer containing a Not I site. Double strand cDNA was size selected according to mRNA size fraction, ligated with EcoR I adaptor, digested with NotI and then cloned directionally into pYX-Asc vector. The library tag sequence located between the Not I site and the polyA tail is TGAGAGAGCC. This library was created for the University Iowa Brain Anatomy Project (BMAP): 'Gene Discovery in the Developing Mouse Nervous System', supported by National Institute of Mental Health (NIMH), Hemin Chin, Ph.D., program coordinator.

text: embryo 12.5dpc

text: DH10B (T1 phage resistant)

text: Brain

characteristic: organ

text: Mus musculus

text: EcoR I

text: Not I

text: whole brain

characteristic: tissue-type

text: Plasmid

text: pYX- Asc


text: live

text: Generic

text: The library was constructed according Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. Denatured RNA was size fractionated on a 1% agarose gel. First strand cDNA synthesis was primed with oligo-dT primer containing a Not I site. Double strand cDNA was size selected according to mRNA size fraction, ligated with EcoR I adaptor, digested with NotI and then cloned directionally into pYX-Asc vector. The library tag sequence located between the Not I site and the polyA tail is GTGCGTGGAA. This library was created for the University Iowa Brain Anatomy Project (BMAP): 'Gene Discovery in the Developing Mouse Nervous System', supported by National Institute of Mental Health (NIMH), Hemin Chin, Ph.D., program coordinator.

characteristic: description

text: embryo 15dpc

text: DH10B (T1 phage resistant)

text: Brain

text: Mus musculus

text: EcoR I

text: Not I

text: whole brain

text: Plasmid

text: pYX- Asc