text: Iowa State University

characteristic: INSDC last update

text: live

text: Generic

text: ds-cDNA molecules were generated as follows. First-strand cDNA was prepared from oligo-dT selected mRNA by priming with an XhoI oligo-dT primer. The resulting DNA:RNA hybrid was treated with RNase H and used as a template for DNA PolI-catalyzed second strand synthesis. After the addition of EcoRI adaptors, the ds-cDNAs were digested with XhoI and size-selected. The resulting molecules were directionally cloned into the EcoRI and XhoI sites of the HybriZAP lambda vector (Stratagene) and excised as pAD-GAL4 phagemids.

text: Two-leaf-stage green seedling

text: XL1-MFR Blue

text: green seedlings

text: Zea mays

text: EcoRI

text: XhoI

text: above ground tissues

text: phagemid


status: PUBLIC

characteristic: External Id

text: Institut des Sciences Vegetales

characteristic: INSDC center name

characteristic: INSDC first public

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Xanthi

tag: attribute

characteristic: cultivar

text: Nicotiana tabacum

characteristic: organism

text: Tobacco mRNA/differential display

characteristic: title


text: University of Tokyo

text: live

text: Generic

text: Xanthi cc

text: Nicotiana tabacum

text: Tobacco mRNA (T.Nagata)


text: The Institute of Physical and Chemical Research (RIKEN)

text: live

text: Generic

text: blastocyst

characteristic: dev-stage

text: Mus musculus

text: Mouse early blastocyst cDNA


text: Max-Planck-Institut fuer Zuechtungsforschung

text: live

text: Generic

text: Zea mays

text: female

characteristic: sex

text: early inflorescence

characteristic: tissue-type

text: Zea mays early female inflorescence

text: lambda-ZAP

characteristic: vector


text: University of Western Australia, Royal Perth Hospital

text: live

text: Generic

text: Mus musculus

text: Mouse HLS5 mRNA


text: University of Chicago Medical Center

text: live

text: Generic

text: promyelocytic leukemia

characteristic: cell type

characteristic: cell-line

text: Homo sapiens

text: HL60 cDNA library

text: pCRII


text: Dalhousie University

text: live

text: Generic

text: cDNA inserts were blunt end cloned into SmaI site of pUC18.

characteristic: description

text: adult

text: brain

characteristic: organ

text: Oreochromis niloticus

text: male

text: Tilapia brain cDNA library in pUC18

text: plasmid


characteristic: External Id

text: Fourth Military Medical University

characteristic: INSDC last update

text: live

text: Generic

text: cloned from SGC-7901 vincristine resistant cell by DDPCR method

text: Homo sapiens

text: gastric cancer

text: Human gastric cancer SGC-7901 cell line


text: STAFF-Institute

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: Sus scrofa

text: back fat

text: Pig back fat


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCATTGCTTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library is normalized, and was constructed by Bento Soares and M.Fatima Bonaldo.

characteristic: lab-host

text: Homo sapiens

text: Not I

text: Eco RI

text: B-cell, chronic lymphotic leukemia

text: phagemid (ampicillin resistant)


text: live

text: Generic

characteristic: NCBI submission package

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCATATCTTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library is normalized, and was constructed by Bento Soares and M.Fatima Bonaldo.

text: brain

text: Homo sapiens

characteristic: organism

text: Not I

text: Eco RI

text: glioblastoma (pooled)

text: phagemid (ampicillin resistant)


text: Washington University School of Medicine

text: live

text: Generic

text: Stanley Neuropathology Consortium (www.stanleylab.org) brains S-58, S-65, S-67, S-78. Random + oligo-dT primed into EcoRI site of ZAP II Vector. Mass excised. Avg insert length 1.9kb. Custom library provided by Dr. Nancy Johnston [(410) 614-3918, nlj@welchlink.welch.jhu.edu].

text: adult

characteristic: dev-stage

text: SOLR (kanamycin resistant)

text: brain

text: Homo sapiens

text: EcoRI

text: male

text: pooled frontal lobe

text: Johnston frontal cortex

characteristic: title

text: phagemid (ampicillin resistant)

text: Bluescript SK-


text: Shanghai Brilliance Biotechnology Institute & Second Military Medical University

text: live

text: Generic

text: monocyte-derived dendritic cells

text: 24 years old

text: Homo sapiens

text: male

characteristic: sex

text: monocyte-derived dendritic cell library


text: University of Arkansas

text: live

text: Generic

text: drought tolerant genotype

text: Gossypium hirsutum

text: leaf

characteristic: tissue-type

text: Cotton drought tolerant genotype, leaf


text: Univ. Paul Sabatier, UMR 5546 CNRS-UPS

text: live

text: Generic

text: cell suspension; isolated with polyclonal antibodies against total protein from either plasma membrane or tonoplast

text: Arabidopsis thaliana

text: Arabidopsis thaliana cell suspension


text: Graduate School of Biotechnology, University of Korea

text: live

text: Generic

text: Dianthus caryophyllus

text: Carnation mRNA


text: Tokyo University of Marine Science and Technology, Graduate School of Marine Science and Technology

text: live

text: Generic

text: Oryzias latipes

text: liver

text: Medaka liver mRNA


text: Deutsches KrebsForschungsZentrum

text: live

text: Generic

text: Xenopus laevis polyA+ RNA embryonal stage 13 were used as source material, and directionally cloned into pBSKS+. For antisense production, plasmid should be cut using SalI, and RNA should be synthesized using T7 RNA polymerase.

text: stage 13

text: E.coli XII blue F+

text: Xenopus laevis

text: SalI

text: NotI

text: whole embryo

text: Xenopus neurula plasmid library

text: Plasmid

text: pBSKS+

characteristic: vector


text: University of Arizona

text: live

text: Generic

text: Six week old

text: Mesembryanthemum crystallinum

text: EcoRI

text: XhoI

text: root, 6h 0.4M NaCl

text: Ice plant Lambda Uni-Zap XR expression library, root, 6h NaCl treatment

text: Phagemid

text: Lambda Uni-Zap XR, Bluescript SK-


text: Beijing Medical University

text: live

text: Generic

text: Mus musculus

characteristic: strain

text: bone marrow

text: Mouse strain C57BL/6 bone marrow


text: live

text: Generic

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCATTTCTTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library went through two rounds of normalization, and was constructed by Bento Soares and M.Fatima Bonaldo.

characteristic: description

text: fetal, mixture of 11.5 and 12.5 dpc

text: gonad

text: Mus musculus

text: Not I

text: Eco RI

text: equal ratio of male:female

text: urogenital ridge (embryonic)

text: Soares mouse urogenital ridge NMUR

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: embryonic stem cell

characteristic: cell type

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCATTGTTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library went through two rounds of normalization, and was constructed by Bento Soares and M.Fatima Bonaldo.

text: Mus musculus

text: Not I

text: Eco RI

text: phagemid (ampicillin resistant)


characteristic: External Id

text: live

text: Generic

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCATAGGTTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library is normalized, and was constructed by Bento Soares and M.Fatima Bonaldo.

text: brain

characteristic: organ

text: Homo sapiens

text: Not I

text: Eco RI

text: anaplastic oligodendroglioma

text: phagemid (ampicillin resistant)


characteristic: INSDC center name

characteristic: INSDC last update

text: live

text: Generic

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACGAATCTGAAGTGGGAGCGGCCGCCCTTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Eco RI adaptors [5' AATTCGGATCCAAC 3' and 5' GTTGGATCCG 3'], digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library constructed by Bob Barstead.

text: adult, age 64

text: Homo sapiens

text: EcoRI

text: NotI

text: male

text: aorta

text: Barstead aorta HPLRB3

text: phagemid (ampicillin resistant)


characteristic: INSDC first public

text: live

characteristic: INSDC status

text: Generic

text: adult, age 89

text: prostate

text: Homo sapiens

text: EcoRI

text: NotI

text: male

text: Barstead prostate BPH HPLRB4

text: phagemid (ampicillin resistant)


text: live

text: Generic

characteristic: NCBI submission model

text: adult, age 67

characteristic: lab-host

text: testis

text: Homo sapiens

text: EcoRI

text: NotI

text: male

text: Barstead testis HPLRB5

text: phagemid (ampicillin resistant)


text: live

text: Generic

characteristic: NCBI submission package

text: adult, age 9 months

text: spleen

text: Mus musculus

characteristic: organism

text: EcoRI

text: NotI

text: male

text: Barstead mouse spleen MPLRB10

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: adult, age 9 months

characteristic: dev-stage

text: Mus musculus

text: EcoRI

text: NotI

text: male

text: Barstead mouse testis MPLRB11

characteristic: title

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: adult, age 9 months

text: Mus musculus

text: EcoRI

text: NotI

text: male

characteristic: sex

text: muscle

characteristic: tissue-type

text: Barstead mouse muscle MPLRB13

text: phagemid (ampicillin resistant)


text: Washington University School of MedicineP

text: live

text: Generic

text: adult, age 9 months

text: Mus musculus

text: EcoRI

text: NotI

text: male

text: Barstead mouse brain MPRB12

text: phagemid (ampicillin resistant)

characteristic: vector


text: Cambridge Institute for Medical Research

text: live

text: Generic

text: Promastigote

text: Leishmania major

text: XhoI

text: NotI

characteristic: strain

text: Leishmania major promastigote full length cDNA library from stationary stage (day 10)

text: Phage

text: Lambda Zap II


text: Instituto de Parasitologia y Biomedicina

text: live

text: Generic

text: epimastigote

characteristic: cell type

text: cDNA library constructed with oligo dT primed epimastigote mRNA and cloned in pt7t318D phagemid with modified polylinker (Pharmacia)

characteristic: description

text: Trypanosoma cruzi

text: EcoRI

text: NotI

text: Cl-Brenner

text: T. cruzi epimastigote normalised cDNA Library


characteristic: External Id

text: Chongqing University of Medical Sciences

text: live

text: Generic

text: Rattus norvegicus

text: Wistar

text: Rat/DD-PCR


text: FMMU

characteristic: INSDC center name

text: live

text: Generic

text: fetus

text: Homo sapiens

text: liver

text: Human fetal liver (J.Zhang)


text: National Research Institute of Aquaculture

characteristic: INSDC last update

text: live

text: Generic

text: Oryzias latipes


text: Louisiana State University Medical Center

characteristic: INSDC first public

text: live

text: Generic

text: endothelium

characteristic: cell-line

text: pigpen(GenBank Accession Number U26024)-binding mRNA

text: fetus

text: Bos taurus

text: aorta

text: Fetal aorta endothelial cell line CPA47


text: Duke University

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: mixed population

text: Caenorhabditis elegans

text: whole animal

text: mRNA from cadmium-responsive gene


text: Medical School Hannover

text: live

text: Generic

characteristic: NCBI submission package

text: Homo sapiens

text: medullary thyroid carcinoma

text: Human medullary thyroid carcinoma


text: Children's Memorial Research Center

text: live

text: Generic

text: The UI-R-C1 library is a subtracted library derived from the UI-R-C0 library, which is a subtracted library derived from the UI-R-A1 and UI-R-E1 libraries. The UI-R-A1 library consisted of a mixture of individually tagged normalized libraries constructed from rat placenta, adult lung, brain, liver, kidney, heart, spleen, ovary, and muscle. The UI-R-E1 library consisted of a mixture of individually tagged normalized libraries constructed from 8, 12 and 18-day embryo. The tag is a string of 3-5 nucleotides present between the Not I site and the oligo-dT track which allows identification of the library of origin of a clone within the mixture. The subtracted library (UI-R-C1) was constructed as follows: PCR amplified cDNA inserts from UI-R-C0 clones from which 3' ESTs had been derived was used as a driver in a hybridization with the UI-R-C0 library in the form of single-stranded circles. The remaining single-stranded circles (subtracted library) was purified by hydroxyapatite column chromatography, converted to double-stranded circles and electroporated into DH10B bacteria (Life Technologies) to generate the UI-R-C1 library. This procedure has been previously described (Bonaldo, Lennon and Soares, Genome Research 6: 791-806, 1996)

text: Adult

text: DH10B (Life Technologies)

characteristic: lab-host

text: Rattus norvegicus

characteristic: organism

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid


text: AgResearch, Ruakura Research Centre

text: live

text: Generic

text: adult

characteristic: dev-stage

text: Ovis aries

text: New Zealand Wiltshire

text: skin

text: Sheep New Zealand Wiltshire breed mRNA

characteristic: title


text: Hiroshima Univ.

text: live

text: Generic

text: Rattus rattus

text: Rat mRNA (K.Yoshizato)


text: The Fourth Military Medical University

text: live

text: Generic

text: mRNA differentially expressed in cell lines GC7901 and GES-1

text: Homo sapiens

text: gastric carcinoma

characteristic: tissue-type

text: Human gastric carcinoma mRNA


text: Horticulture Research International

text: live

text: Generic

text: Brighton

characteristic: cultivar

text: Fragaria x ananassa

text: receptacle

text: phage

text: lambda gt10

characteristic: vector


text: University of Sydney

text: live

text: Generic

text: from 3 week old cotton (cultivar Sicala V-1) seedling root; 17 days in axenic culture with the arbuscular mycorrhizal fungus Gigaspora margarit

text: Gossypium hirsutum/Gigaspora margarita mixed EST library


text: University of Magdeburg 'Otto von Guericke'

text: live

text: Generic

text: primary hippocampal cell culture

characteristic: cell type

text: Rattus norvegicus

text: brain

text: Rat primary hippocampal cell culture


text: Tokyo University of Agriculture and Technology

text: live

text: Generic

text: two week old

text: Mesembryanthemum crystallinum

text: EcoRI

text: EcoRI

text: seedling

text: Ice plant seedlings, RT-PCR , pCRII

text: plasmid

text: pCRII


text: Mott Center for Human Growth and Development, Wayne State University School of Medicine

text: live

text: Generic

text: Differential display product obtained by using anchored oligo dT primer: deoxyTTTTTTTTTTTTVC (V: Equimolar mixture of dA, dC and dG) and an arbitrary primer: 5'CTTTCTACCC 3' (Both from Operon Technology, Inc.). Reamplified product using the same primer set was cloned into pCR3 vector (Invitrogen)

characteristic: description

text: blastocyst

text: Mus musculus

text: Mouse blastocyst differential display product/primer CTTTCTACCC

text: pCR3 vector (Invitrogen)


characteristic: External Id

text: Ludwig Institute of Cancer Research

text: live

text: Generic

text: fibroblast

text: IFN-alpha induced primary cells

text: 14 day embryo

text: Mus musculus

characteristic: strain

text: Mouse IFN-alpha induced primary cells


text: Cardiovascular Institute & Fu Wai Hospital

characteristic: INSDC center name

characteristic: INSDC last update

text: live

text: Generic

text: adult

text: Homo sapiens

text: male

characteristic: sex

text: aorta

text: Human adult aorta


text: Institute of General Genetics

characteristic: INSDC first public

text: live

characteristic: INSDC status

text: Generic

text: fetal

text: Homo sapiens

text: brain

text: Human fetal brain (Zakharev,V.M. and Belyavsky,A.V.)


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: mRNA made from papillary serous ovarian carcinoma, cDNA made by oligo-dT priming. Directionally cloned. Size-selected on agarose gel, average insert size 500 bp. Primary library, non-amplified.

text: adult

text: ovary

characteristic: organ

text: Homo sapiens

text: female

text: papillary serous carcinoma

text: plasmid (ampicillin resistant)


text: Clemson University

text: live

text: Generic

text: This is a Uni-ZAP XR custom cDNA library made by Stratagene (U.S.A.: 1-800-424-5444): Stratagene cat. #837201.

text: XL1-Blue MRF'

characteristic: lab-host

text: Gossypium hirsutum

characteristic: organism

text: EcoRI

text: XhoI

text: Cotton Boll Abscission Zone cDNA Library

text: Phage

text: Uni-ZAP XR


text: INSERM U 254

text: live

text: Generic

text: outer hair cells

text: day 13 post-natal

characteristic: dev-stage

text: Rattus norvegicus

text: Wistar

text: cochlea

characteristic: tissue-type

text: Rat cochlea outer hair cells Lambda Zap Express Library

characteristic: title


text: Universite de Sherbrooke

text: live

text: Generic

text: obtained from differential display of total RNA obtained from intestinal surgical resections of patients with Crohn's disease

text: Homo sapiens

text: intestine

text: Human intestine from Crohn's disease patients


text: Institute of Neurobiology, University Heidelberg

text: live

text: Generic

text: Provided by Jose Campos-Ortega, University of Cologne

text: embryonic 22 hours post-fertilization

text: Danio rerio

text: Danio rerio 22h cDNA Library


text: Beijing Institute of Radiation Medicine

text: live

text: Generic

text: fetal

text: Homo sapiens

text: liver

text: Human fetal liver cDNA library

text: Plasmid

characteristic: vector


text: Lawrence Berkeley National Lab

text: live

text: Generic

text: Sized fractionated cDNAs were directly ligated into pOT2. Plasmid cDNA library.

characteristic: description

text: adult

text: DH5 - alpha

text: head

text: Drosophila melanogaster

text: EcoRI

text: XhoI

text: male and female

text: GH Drosophila melanogaster head pOT2


characteristic: External Id

text: Chinese Academic of Medical Sciences & PUMC

text: live

text: Generic

text: 6 weeks

text: XL1-Blue MRF'

text: Rattus norvegicus

text: EcoR I

text: Xho I

text: Male

text: Sprague-Dawley

text: brain

text: Rat Lambda Zap II Library, Stratagene #936501

text: Phage

text: Lambda Zap II


text: Cold Spring Harbor Laboratory

text: live

text: Generic

characteristic: cultivar

text: This library is described in Schmidt, Hake, et al., (1993) Plant Cell 5:729-737. cDNAs are directionally cloned into the XhoI and EcoRI sites; XhoI is near the polyA tail. Most reads from this library are 3' in direction. Additional information on this library as well as ftp access to all sequences can be found at http://www.cshl.org/maizegenome

text: Zea mays

text: XhoI

text: EcoRI

text: female

text: immature ear

text: maize inflorescence immature ear library

text: plasmid

text: pBLUESCRIPT SK+ (X52325)


text: UMMC

characteristic: INSDC center name

text: live

text: Generic

text: Macaca mulatta

text: endometrium

text: Rhesus monkey endometrium


text: Institute of Radiation Medicine

characteristic: INSDC last update

text: live

text: Generic

text: obtained by differential display of mRNA from lung cancer tissue of Yunnan Tin miners

text: Homo sapiens

text: lung cancer

text: Yunnan Tin miner lung cancer tissue mRNA


text: University of Tokyo

text: live

text: Generic

text: androgen-independent Shionogi carcinoma cells

characteristic: cell type

text: Mus musculus


text: Yamaguchi University

characteristic: INSDC first public

text: live

text: Generic

text: HeLa

characteristic: cell-line

text: Homo sapiens

text: HeLa cDNA (T.Noma)


text: Yamaguchi University

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Drosophila melanogaster

text: Drosophila melanogaster cDNA (T.Noma)


text: Smith College Department of Biological Sciences

text: live

text: Generic

characteristic: NCBI submission package

text: Lymphatic filarial nematode parasite of humans. cDNA from L3, microfilaria, adult male and adult female libraries was isolated and amplified by PCR using T3 and T7 primers. Microfilaria, adult male and adult female cDNA was subtracted from L3 cDNA using the PCR-select cDNA subtraction kit (Clontech). The library had 4.0 x 105 independent recombinants and the average insert size is ~500bp. The library was constructed by Lori Saunders. The library is available from Dr. Steven A. Williams, email: genome@smith.edu

text: L3 cDNA subtracted with microfilaria, adult male and adult female cDNA libraries

text: XL1-Blue MRF'

text: Brugia malayi

text: Eco RI

text: Eco RI

text: Brugia malayi L3 subtracted cDNA library (SAW97LS-BmL3SD)

text: Phage

text: Lambda ZAP II


text: live

text: Generic

text: Filarial nematode parasite of humans. Six adult male worms of Onchocerca volvulus were isolated from consenting patients and quick frozen. Adult male mRNA was converted to double-stranded cDNA using reverse transcriptase and oligo(dT) followed by RNase H and DNA pol I. The library has 2 x 10E5 independent recombinants and the average insert size is ~1100bp.The library was constructed by Michelle Lizotte-Waniewski with worms provided by Dr. Sara Lustigman. The library is available from Dr. Steven A. Williams, email: genome@smith.edu.

text: adult

text: XL1-Blue MRF'

characteristic: lab-host

text: Onchocerca volvulus

text: Eco RI

text: Xho I

text: male

characteristic: sex

text: Onchocerca volvulus adult male cDNA (SAW98MLW-OvAM)

text: Phage

text: Lambda Uni-ZAP XR


text: live

text: Generic

text: Filarial nematode parasite of humans. Two adult female worms of Onchocerca volvulus were isolated from consenting patients and quick frozen. Adult female mRNA was converted to double-stranded cDNA using reverse transcriptase and oligo(dT) followed by RNase H and DNA pol I. The library has 7 x 10E5 independent recombinants and the average insert size is ~1100bp.The library was constructed by Michelle Lizotte-Waniewski with worms provided by Dr. Sara Lustigman. The library is available from Dr.Steven A. Williams, email: genome@smith.edu.

text: adult

characteristic: dev-stage

text: XL1-Blue MRF'

text: Onchocerca volvulus

characteristic: organism

text: Eco RI

text: Xho I

text: female

text: Onchocerca volvulus adult female cDNA (SAW98MLW-OvAF)

text: Phage

text: Lambda Uni-ZAP XR


text: University of Texas at Austin

text: live

text: Generic

text: B-lymphoblastoid

text: RECC-UTC4-1, derived in the lab of Dr. Henry Bose, UT-Austin

text: spleen

characteristic: organ

text: Gallus gallus

text: SPAFAS

characteristic: strain

text: differential display sequences cloned into pCR2.1

characteristic: title


characteristic: External Id

text: Mott Center for Human Growth and Development, Wayne State University School of Medicine

text: live

text: Generic

text: amplified using primer sequences from mouse alpha IIb integrin subunit and cloned into PCR II vector (Invitrogen)

characteristic: description

text: blastocyst

text: Mus musculus

text: NSA

text: Mouse blastocyst cDNA RT-PCR amplified

text: PCR II

characteristic: vector


characteristic: INSDC center name

characteristic: INSDC last update

text: live

text: Generic

text: 26 week fetus

text: Homo sapiens

text: brain

characteristic: tissue-type

text: Human brain mRNA (U.K.Rout)


text: Japan Science and Technology Corporation (JST)

characteristic: INSDC first public

text: live

text: Generic

text: eight-cell stage embryo

text: Mus musculus

text: Mouse eight-cell stage embryo cDNA


text: live

characteristic: INSDC status

text: Generic

text: two-cell stage embryo

text: Mus musculus

text: Mouse two-cell stage embryo cDNA


text: University of Texas at Austin

text: live

text: Generic

characteristic: NCBI submission model

text: b-lymphoblastoid

characteristic: cell type

text: RECC-UTC4-1 (C4-1) derived in the lab of Dr. Henry Bose, UT-Austin

characteristic: cell-line

text: spleen

text: Gallus gallus

text: SPAFAS

text: C4-1 RACE sequences cloned into pCR2.1


text: State University of New York at Buffalo

text: live

text: Generic

characteristic: NCBI submission package

text: Schistosoma mansoni

text: EcoRI

text: XhoI

text: Schistosoma mansoni, Phil LoVerde/Joe Merrick

text: pBluescript SK


text: Clemson University

text: live

text: Generic

text: The appressorium formation-specific cDNA library was constructed form conidia germinated for 5-8 hr on an inductive surface. The library has an average insert size of 1.5 kbp.

text: Germinated conidia on appressorium-inductive surface

characteristic: dev-stage

text: Pyricularia grisea

text: EcoRI

text: XhoI

text: Magnaporthe grisea Appressorium Stage cDNA Library

text: Plasmid

text: pBlueScriptII SK(+) Vector


text: Children's Memorial Research Center

text: live

text: Generic

text: The UI-R-C2 library is a subtracted library derived from the UI-R-C1 library, which is a subtracted library derived from the UI-R-C0 library. The UI-R-C0 library consisted of a mixture of individually tagged normalized libraries constructed from rat placenta, adult lung, brain, liver, kidney, heart, spleen, ovary, muscle, 8, 12 and 18-day embryo. The tag is a string of 3-5 nucleotides present between the Not I site and the oligo-dT track which allows identification of the library of origin of a clone within the mixture. The subtracted library (UI-R-C2) was constructed as follows: PCR amplified cDNA inserts from UI-R-C1 clones from which 3' ESTs had been derived was used as a driver in a hybridization with the UI-R-C1 library in the form of single-stranded circles. The remaining single-stranded circles (subtracted library) was purified by hydroxyapatite column chromatography, converted to double-stranded circles and electroporated into DH10B bacteria (Life Technologies) to generate the UI-R-C2 library. This procedure has been previously described (Bonaldo, Lennon and Soares, Genome Research 6: 791-806, 1996)

text: adult

text: DH10B (Life Technologies)

characteristic: lab-host

text: Rattus norvegicus

characteristic: organism

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid


text: Children's Memorial Research Center

text: live

text: Generic

text: The UI-R-Y0 library is a subtracted library derived from an individually-tagged normalized whole-eye (minus the lens) library. The driver for the subtraction consisted of a pool of all previous libraries (UI-R-A0, UI-R-A1, UI-R-E0, UI-R-E1, UI-R-C0, and UI-R-C1). The tag is a string of 3-5 nucleotides present between the Not I site and the oligo-dT track which allows identification of the library of origin of a clone within the mixture. The subtracted library (UI-R-Y0) was constructed as follows: PCR amplified cDNA inserts from previous library clones from which 3' ESTs had been derived were used as a driver in a hybridization with the normalized whole-eye library in the form of single-stranded circles. The remaining single-stranded circles (subtracted library) was purified by hydroxyapatite column chromatography, converted to double-stranded circles and electroporated into DH10B bacteria (Life Technologies) to generate the UI-R-Y0 library. This procedure has been previously described (Bonaldo, Lennon and Soares, Genome Research 6: 791-806, 1996)

text: adult

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

characteristic: strain

text: Phagemid


text: Shanghai Institute of Cell Biology

text: live

text: Generic

text: heart

characteristic: organ

text: Rattus norvegicus

text: male

characteristic: sex

text: Wistar Kyoto

text: cardiac tissue

text: Wistar Kyoto Rat heart

characteristic: title

text: pCRII (Invitrogen)


characteristic: External Id

text: live

text: Generic

text: unfertilized egg

text: Mus musculus

text: Mouse unfertilized egg cDNA


text: Universidade Federal de Minas Gerais

characteristic: INSDC center name

text: live

text: Generic

text: L929 cells treated with interferon and infected with vaccinia virus

characteristic: description

text: Mus musculus

text: Mouse L929 cells


text: Life Science College of Wuhan University

characteristic: INSDC last update

text: live

text: Generic

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer; double-stranded cDNA was ligated to Sal I adaptors. After digestion with Not I, the cDNA was ligated into Sal I-Not I-cut pSPORT1 plasmid and electroporated into high-efficency electrocompetent coli DH10B (Life Technologies) using the Bio-Rad GenePulser. Library has an average insert length of 1.6 kb. Library constructed by Deming Gou, Huang Jian, Li Wenxin.

text: About 3-week-old embryo after fertilization

text: Homo sapiens

text: Not I

text: Sal I

text: Whole embryo

characteristic: tissue-type

text: human embryo cDNA library

text: Plasmid

characteristic: vector


text: Pohang University of Science and Technology

characteristic: INSDC first public

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: guard cell protoplast

text: Brassica rapa subsp. pekinensis

text: Brassica rapa guard cell


text: Tohoku Univ. School of Med.

text: live

text: Generic

characteristic: NCBI submission package

text: kidney

text: Homo sapiens

text: human kidney (N.Fuse)


text: University of Guelph

text: live

text: Generic

text: Medicago sativa

text: shoot tip and expanding leaves

text: Medicago sativa shoot tip Lambda ZAP cDNA library


text: Yale University School of Medicine

text: live

text: Generic

text: EPRO

characteristic: cell-line

text: isolated by cDNA RDA of the committed myeloid progenitor cell line, EPRO

text: Mus musculus

text: Mouse cell line EPRO


text: Univ. and Cantonal Hospital of Geneva

text: live

text: Generic

text: Homo sapiens

text: Human trapped exon (S.E.Antonarakis)


text: live

text: Generic

text: heart

text: Rattus norvegicus

text: ventricle

text: embryonic day 17 cDNA


text: live

text: Generic

text: neonatal 10 day

characteristic: dev-stage

text: heart

text: Rattus norvegicus

text: ventricle

text: neonatal 10 day cDNA


text: Massachusetts General Hospital

text: live

text: Generic

text: adult

text: E. coli XL1 Blue

characteristic: lab-host

text: heart

text: Danio rerio

characteristic: organism

text: mixed

text: AB

text: myocardium, endocardium, vessel

text: ZF adult heart library

text: Phage

text: LambdaZAPII


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: Plasmid DNA from the normalized library NCI_CGAP_GC4 was prepared, and ss circles were made in vitro. Following HAP purification, this DNA was used as tracer in a subtractive hybridization reaction. The driver was PCR-amplified cDNAs from a pool of 5,000 clones made from the same library (cloneIDs 1257096-1258631, 1469064-1470983, and 1475592-1476743). Subtraction by Bento Soares and M. Fatima Bonaldo.

text: Homo sapiens

text: Not I

text: Eco RI

text: pooled germ cell tumors

text: phagemid (ampicillin resistant)


text: Korea University

text: live

text: Generic

text: Hanbyul

characteristic: cultivar

text: Capsicum annuum

text: leaf

text: Capsicum annuum leaf mRNA


text: Washington University School of Medicine

text: live

text: Generic

text: Double-stranded cDNA was prepared from human astrocytoma using primer 5'-GAGAGAGAGAGAGAGAGAGAAACTAGTCTGAGT(18)-3'. An EcoRI adaptor was used on the 5' end of the cDNA as follows: 5'-AATTCGGCACGAG-3'. The library was size-selected and went through one round of amplification. Average insert size is 1.7 kb, with a range from 0.4-12 kb. Tumor identification by consensus pathology. This library was constructed by Dr. Martin Schiller (Johns Hopkins University).

text: 44 years

text: SOLR

text: brain

characteristic: organ

text: Homo sapiens

text: EcoRI

text: XhoI

text: male

characteristic: sex

text: astrocytoma

text: Schiller astrocytoma

text: plasmid (ampicillin resistant)

text: pBluescript SK- (Stratagene)


characteristic: External Id

text: live

text: Generic

text: Double-stranded cDNA was prepared from human glioblastoma multiforme using primer 5'-GAGAGAGAGAGAGAGAGAGAAACTAGTCTGAGT(18)-3'. An EcoRI adaptor was used on the 5' end of the cDNA as follows: 5'-AATTCGGCACGAG-3'. The library was size-selected and went through one round of amplification. Average insert size is 1.7 kb, with a range from 0.4-12 kb. Tumor identification by consensus pathology; shows LOH on chromosomes 10 and 14 and a gain in 9p and 1q. The tumor also has a heterozygous p53 mutation R273C. This library was constructed by Dr. Martin Schiller (Johns Hopkins University).

text: 33 years

text: SOLR

text: brain

text: Homo sapiens

text: EcoRI

text: XhoI

text: female

text: glioblastoma multiforme

text: Schiller glioblastoma multiforme

characteristic: title

text: plasmid (ampicillin resistant)

text: pBluescript SK- (Stratagene)


characteristic: INSDC center name

text: live

text: Generic

text: Double-stranded cDNA was prepared from human meningioma using primer 5'-GAGAGAGAGAGAGAGAGAGAAACTAGTCTGAGT(18)-3'. An EcoRI adaptor was used on the 5' end of the cDNA as follows: 5'-AATTCGGCACGAG-3'. The library was size-selected and went through one round of amplification. Average insert size is 1.7 kb, with a range from 0.4-12 kb. Tumor identification by consensus pathology. This library was constructed by Dr. Martin Schiller (Johns Hopkins University).

characteristic: description

text: 72 years

text: SOLR

text: brain

text: Homo sapiens

text: EcoRI

text: XhoI

text: female

text: meningioma

text: Schiller meningioma

text: plasmid (ampicillin resistant)

text: pBluescript SK- (Stratagene)


characteristic: INSDC last update

text: live

text: Generic

text: Double-stranded cDNA was prepared from human oligodendroglioma using primer 5'-GAGAGAGAGAGAGAGAGAGAAACTAGTCTGAGT(18)-3'. An EcoRI adaptor was used on the 5' end of the cDNA as follows: 5'-AATTCGGCACGAG-3'. The library was size-selected and went through one round of amplification. Average insert size is 1.7 kb, with a range from 0.4-12 kb. Tumor identification by consensus pathology; contains chromosome 1p and 19q deletion as determined by CGH. This library was constructed by Dr. Martin Schiller (Johns Hopkins University).

text: 44 years

text: SOLR

text: brain

text: Homo sapiens

text: EcoRI

text: XhoI

text: male

text: oligodendroglioma

characteristic: tissue-type

text: Schiller oligodendroglioma

text: plasmid (ampicillin resistant)

text: pBluescript SK- (Stratagene)


text: Washington University School of MedicineP

characteristic: INSDC first public

text: live

text: Generic

text: colon cancer cell line MAC13

text: Double-stranded cDNA was prepared from cell line MAC13 using primer 5'-GAGAGAGAGAGAGAGAGAGAAACTAGTCTGAGT(18)-3'. An EcoRI adaptor was used on the 5' end of the cDNA as follows: 5'-AATTCGGCACGAG-3'. The library was size-selected and went through one round of amplification. Average insert size is 1.7 kb, with a range from 0.4-12 kb. This library was constructed by Dr. Martin Schiller (Johns Hopkins University).

text: SOLR

text: Mus musculus

text: EcoRI

text: XhoI

text: colon cancer

text: Schiller mouse MAC13

text: plasmid (ampicillin resistant)

text: pBluescript SK- (Stratagene)

characteristic: vector


text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Plasmid DNA from the normalized library NCI_CGAP_Pr22 was prepared, and ss circles were made in vitro. Following HAP purification, this DNA was used as tracer in a subtractive hybridization reaction. The driver was PCR-amplified cDNAs from a pool of 5,000 clones made from the same library (cloneIDs 985608-986759, 1101192-1101959, and 1217928-1220615). Subtraction by Bento Soares and M. Fatima Bonaldo.

text: adult

text: prostate

text: Homo sapiens

text: male

text: phagemid (ampicillin resistant)


text: The Natural History Museum

text: live

text: Generic

characteristic: NCBI submission package

text: female enriched, subtractive library in Lambda ZAPII, 5% female-enriched. Constructed by Anja Michel, Heinrich Heine University, Duesseldorf. Excised into pBluescript for EST generation. Contact Werner Kunz (Werner.Kunz@uni-duesseldorf.de) for further information

text: sexually mature adult

text: Mus musculus (definitive host), Biomphalaria glabrata (intermediate host)

text: Schistosoma mansoni

text: EcoRI

text: XhoI

text: female

text: whole organism

text: S. mansoni, female enriched, subtractive, HHU(A)

text: Phage

text: Lambda ZAPII


text: Royal Postgraduate Medical School

text: live

text: Generic

text: adult

characteristic: dev-stage

text: Rattus norvegicus

text: Wistar

characteristic: strain

text: rat cDNA (S.Legon)


text: Children's Memorial Research Center

text: live

text: Generic

text: The UI-R-C2p library is a subtracted library derived from the UI-R-C1 library, which is a subtracted library derived from the UI-R-C0 library. The UI-R-C0 library consisted of a mixture of individually tagged normalized libraries constructed from rat placenta, adult lung, brain, liver, kidney, heart, spleen, ovary, muscle, 8, 12 and 18-day embryo. The tag is a string of 3-5 nucleotides present between the Not I site and the oligo-dT track which allows identification of the library of origin of a clone within the mixture. The subtracted library (UI-R-C2p) was constructed as follows: PCR amplified cDNA inserts from UI-R-C1 clones from which 3' ESTs had been derived was used as a driver in a hybridization with the UI-R-C1 library in the form of single-stranded circles. The remaining single-stranded circles (subtracted library) was purified by hydroxyapatite column chromatography, converted to double-stranded circles and electroporated into DH10B bacteria (Life Technologies) to generate the UI-R-C2p library. This procedure has been previously described (Bonaldo, Lennon and Soares, Genome Research 6: 791-806, 1996)

text: adult

text: DH10B (Life Technologies)

characteristic: lab-host

text: Rattus norvegicus

characteristic: organism

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid


text: UCLA-School of Dentistry

text: live

text: Generic

text: oral keratinocytes

characteristic: cell type

text: Homo sapiens

text: Human oral keratinocytes


text: Eccles Institute of Human Genetics, University of Utah

text: live

text: Generic

text: see Brown, N.H. and Kafatos, F.C. (1988) Functional cDNA libraries from Drosophila embryos. J. Mol Biol. 203, 425-37.

text: embryonic 8-12 hr post-fertilization

text: Drosophila melanogaster

text: Drosophila 8-12 hr embryonic cDNA library

text: plasmid pNB


text: Institute of Basic Medical Sciences, Peking Union Medical College & Chinese Academy of Medical Sciences

text: live

text: Generic

text: B lymphocytes were isolated from excised tonsils, activated with PMA(5ng/ml). Total RNA and mRNA were isolated from activated B lymphocytes. A cDNA library was constructed with SuperScript Plasmid System for cDNA synthesis and Plasmid Cloning (GIBCO BRL). Then single-stranded DNA(ssDNA) of the library was prepared with M13 helper phage and hybridized with cDNA of resting lymphocytes. Double-stranded DNA(dsDNA) was removed with hydroxyapatite and the remaining ssDNA was repaired to dsDNA and transformed E. coli JM109. Clones with long inserts were further studied.

text: E. coli JM109

text: tonsil

characteristic: organ

text: Homo sapiens

text: NotI

text: SalI

text: Subtracted cDNA library of activated B lymphocyte

text: Phagemid


characteristic: External Id

text: live

text: Generic

text: Activated B Cell

characteristic: cell-line

text: 3D5 cell is an immortal EB virus-transformed human B cell line. Monoclonal antibody GB1 specially recognize a 43 kD protein that is evidently expressed on activated human B cell and extensively on 3D5 cell. A cDNA library of 3D5 cell was screened with GB1 and a new sequence was obtained.

text: E. coli Y1090

text: Homo sapiens

text: EcoRI

text: cDNA library of activated B cell line 3D5

text: Phage


text: International Livestock Research Institute

characteristic: INSDC center name

text: live

text: Generic

text: Cultured bovine lymphocytes infected with Theileria parva

text: A cDNA library enriched in T. parva schizonts was constructed using mRNA derived from cultured bovine lymphoblastoid cells that were infected with T. parva Mguga schizonts. Theileria parva schizonts from 10 million infected bovine macrophages were concentrated by centrifugation and total RNA was extracted from this schizonts-enriched fraction using the hot phenol-SDS method, (Murphy and Pelle, 1995). Poly-A+ RNA was obtained from the total RNA by oligo-dT cellulose (Phamacia Biotech) affinity chromatograph. Complementary DNA was synthesised from the poly-A+ RNA using the RiboClone cDNA Synthesis Systems (Promega) and standard methods. To facilitate cloning, the cDNAs were methylated using EcoRI methylase and ligated to oligonucleotide adapters containing the EcoRI site (GGGGGAATTCGGGGG) using T4 DNA ligase (NEB). The cDNAS were then treated with the restriction endonuclease EcoRI (NEB), purified and then ligated to lgt11 bacteriophage vector arms (Promega) digested with the same enzyme. Recombinant lgt11 phage DNA was packaged into phage capsids using the Packagene l-DNA packaging mixture (Promega) and amplified by infecting E. coli, strain Y1090 cells.

text: Schizont stage

text: Theileria parva

text: Muguga

text: Bovine lymphocytes

text: Schizont Lambda gt11 cDNA Library

characteristic: title


text: Mott Center for Human Growth and Development, Wayne State University School of Medicine

characteristic: INSDC last update

text: live

text: Generic

text: liver

text: Mus musculus

text: Mouse liver RT-PCR cloned


text: live

text: Generic

text: Homo sapiens

text: placenta

characteristic: tissue-type

text: Human placenta RT-PCR amplified cDNA


text: University of Toronto

characteristic: INSDC first public

text: live

text: Generic

text: B cell large cell lymphoma

text: OCI LY8-C3P; clonally related variant of C3-A11N

text: Homo sapiens

text: Human OCI LY8-C3P


text: University of Toronto

text: live

text: Generic

text: B cell large cell lymphoma

text: C3-A11N; clonally related variant of OCI LY8-C3P

text: Homo sapiens

text: Human C3-A11N


text: University of Glasgow

text: live

text: Generic

text: embryo 12.5 dpc

text: Mus musculus

text: whole embryo

text: Mouse embryo 12.5 dpc

text: pBluescript KSII-

characteristic: vector


text: Pusan National University

text: live

characteristic: INSDC status

text: Generic

text: ExAssist helper phage with SOLR strain is designed to allow efficient in vivo excision of the pBluescript phage from the Uni-ZAP XR vector while preventing the problems that can be associated with helper phage co-infection

characteristic: description

text: gametophyte

text: SOLR

text: Neopyropia yezoensis

text: EcoRI

text: XhoI

text: wild type in Pohang seashore

text: vegetative tissue except hold fast

text: Porphyra yezoensis in Lambda UniZAP

text: phagemid

text: Lambda UniZAP


text: Children's Memorial Research Center

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: This library (UI-R-BT0) consists of a mixture of individually tagged normalized libraries constructed from rat hippocampus, thalamus, mid-brain, medulla, corpus striatum, cerebral cortex and testis. The tag used to identify the source tissue is a string of 3-6 nucleotides present between the Not I site and the oligo-dT track which allows identification of the library of origin of a clone within the mixture. This library was then subtracted using a driver consisting of a mixture of all clones from UI-R-A0, UI-R-A1, UI-R-E0, UI-R-E1, UI-R-C0, UI-R-C1, UI-R-C2 and UI-R-C2p.

text: adult

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid


text: live

text: Generic

text: embryo

characteristic: dev-stage

text: Gallus gallus

text: White Leghorn

characteristic: strain

text: Chicken Lambda gT11 library

text: Phage

text: lambda gt11


text: University of Illinois at Urbana-Champaign

text: live

text: Generic

text: The library was prepared by Stratagene using oligo-T priming and unidirectional cloning with an adaptor at the 5' end (GGCACGAG) following the EcoRI site. The mRNA was prepared from antennae of late pupal and newly eclosed male moths. Clones were subcloned in vivo in mass into pBluescript maintained in SOLR cells for DNA sequencing.

text: newly eclosed adults and pharate adults

text: XL1 Blue MRF and SOLR

characteristic: lab-host

text: antennae

text: Manduca sexta

characteristic: organism

text: EcoRI

text: XhoI

text: male

characteristic: sex

text: Manduca sexta male antennae Uni-ZAP XR library

text: phage

text: Uni-ZAP XR


characteristic: External Id

text: Institut Pasteur

text: live

text: Generic

text: Mus musculus

text: Mouse cDNA (S.Herblot)


text: NIAID

text: live

text: Generic

text: B cells treated with LPS and IL-4 for 12 and 36 hours

characteristic: cell type

text: Mus musculus

text: BALB/c

text: spleen

text: Mouse B cells treated with LPS and IL-4


text: Centro de Pesquisas Rene Rachou - FIOCRUZ

characteristic: INSDC center name

text: live

text: Generic

text: mRNA was extracted from eggs and the library was constructed and excised according to the manufacturer's instructions.

text: Egg

text: Schistosoma mansoni

text: EcoRI

text: XhoI

text: Mixed

text: LE

text: Egg stage cDNA expression library in Lambda ZAPII

text: Uni-Zap XR vector, Stratagene (pBluescript SK).


text: live

text: Generic

text: The library was prepared by Stratagene using random and oligo-T priming and unidirectional cloning. Clones were subcloned in vivo into pBluescript maintained in SOLR cells for DNA sequencing.

text: XL1 Blue MRF' and SOLR

text: last larval instar nerve cords and developing adult wings

characteristic: organ

text: Manduca sexta

text: EcoRI

text: XhoI

text: male and female

text: Manduca sexta larval nerve cord and adult wing Uni-ZAP XR library

characteristic: title

text: phage

text: Uni-ZAP XR


text: Columbia University, P & S

characteristic: INSDC last update

text: live

text: Generic

text: nude mouse tumor derived H5ts125-transformed secondary embryo cell

characteristic: cell-line

text: Rattus norvegicus

text: Sprague-Dawley

text: Lambda Zap cDNA Library


characteristic: INSDC first public

text: live

text: Generic

text: brain

text: Drosophila melanogaster

text: Drosophila melanogaster brain mRNA


text: University of Wisconsin-Madison

text: live

characteristic: INSDC status

text: Generic

text: limonene-treated regressing tumor cDNA library subtracted from a control-treated non-regressing cDNA library

text: Rattus norvegicus

text: Wistar-Furth

text: 7,12-dimethylbenz[a]anthracene (DMBA)-induced mammary carcinoma

characteristic: tissue-type

text: Rat limonene-treated regressing tumor cDNA library


text: University of Houston

text: live

text: Generic

characteristic: NCBI submission model

text: adult

text: brain

text: Drosophila melanogaster

text: Drosophila melanogaster adult brain cDNA


text: Shanghai Institute of Cell Biology, Chinese Academy of Sciences

text: live

text: Generic

characteristic: NCBI submission package

text: liver

text: Homo sapiens

text: Human liver EST (Y.L.Yu)


text: Umea University

text: live

text: Generic

text: Cambial region tissues, including developing xylem, the meristematic cambial zone and the developing and mature phloem, was harvested from 1.5 m actively growing trees. cDNA was prepared and cloned into lambda gt22a. DNA was isolated and subcloned into pBluescript SK using SalI and NotI restriction enzymes.

characteristic: description

text: 1.5 m actively growing tree

characteristic: dev-stage

text: E.coli

text: Populus tremula x Populus tremuloides

text: SalI

text: NotI

text: Cambial region

text: Hybrid aspen plasmid library

text: Plasmid

text: pBluescript SK

characteristic: vector


text: Flanders Interuniversity Institute for Biotechnology,University of Gent

text: live

text: Generic

text: Trichobel

characteristic: cultivar

text: Young developing xylem was harvested from 3-year-old, actively growing Populus trichocarpa 'Trichobel' by peeling off the bark first and then scraping on the remaining xylem. cDNA was prepared and cloned directionally into Lambda ZAPII. Plasmid clones of individual Lamdda clones were obtained by in vivo excision.

text: 3-year-old actively growing tree (harvested in May)

text: E.coli

characteristic: lab-host

text: Populus trichocarpa

characteristic: organism

text: EcoRI (5' end cDNA)

text: XhoI (3' end cDNA)

text: Young, developing xylem

text: Poplar xylem Lambda ZAPII library

text: Plasmid

text: pBluescript SK


characteristic: External Id

text: Centro de Pesquisas Rene Rachou - FIOCRUZ

text: live

text: Generic

text: library was constructed and excised according to the manufacturer's instructions.

text: adult

text: Schistosoma mansoni

text: EcoRI

text: XhoI

text: Mixed

characteristic: sex

text: LE

characteristic: strain

text: Adult worm cDNA expression library

text: Uni-Zap XR vector, Stratagene (pBluescript SK)


text: Washington University School of Medicine

characteristic: INSDC center name

text: live

text: Generic

text: pituitary cell line

text: Double-stranded cDNA was prepared from cell line AtT-20 using primer 5'-GAGAGAGAGAGAGAGAGAGAAACTAGTCTGAGT(18)-3'. An EcoRI adaptor was used on the 5' end of the cDNA as follows: 5'-AATTCGGCACGAG-3'. The library was size-selected and went through one round of amplification. Average insert size is 1.7 kb, with a range from 0.4-12 kb. This library was constructed by Dr. Martin Schiller (Johns Hopkins University).

text: SOLR

text: pituitary

characteristic: organ

text: Mus musculus

text: EcoRI

text: XhoI

text: Schiller mouse AtT20

characteristic: title

text: plasmid (ampicillin resistant)

text: pBluescript SK- (Stratagene)


characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: pituitary cell line

characteristic: cell-line

text: Double-stranded cDNA was prepared from cell line AtT-20 using primer 5'-GAGAGAGAGAGAGAGAGAGAAACTAGTCTGAGT(18)-3'. An EcoRI adaptor was used on the 5' end of the cDNA as follows: 5'-AATTCGGCACGAG-3'. The library was size-selected and went through one round of amplification. Average insert size is 1.7 kb, with a range from 0.4-12 kb. This cell line was engineered for inducible overexpression of RESP18 using the rTET system. Cells were induced such that RESP18 protein would traverse the ER to the Golgi and induce the RESP18-activated signaling pathway. This library was constructed by Dr. Martin Schiller (Johns Hopkins University).

text: SOLR

text: pituitary

text: Mus musculus

text: EcoRI

text: XhoI

text: Schiller mouse AtT20 RESP induced

text: plasmid (ampicillin resistant)

text: pBluescript SK- (Stratagene)


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: mRNA made from breast carcinoma, cDNA made by oligo-dT priming. Non-directionally cloned. Size-selected on agarose gel, average insert size 500 bp. Non-amplified library.

text: breast

text: Homo sapiens

text: female

text: invasive carcinoma

characteristic: tissue-type

text: plasmid (ampicillin resistant)


text: live

text: Generic

characteristic: NCBI submission package

text: mRNA made from breast carcinoma, cDNA made by oligo-dT priming. Directionally cloned. Size-selected on agarose gel, average insert size 500 bp. Primary library, non-amplified.

characteristic: description

text: adult

characteristic: dev-stage

text: breast

text: Homo sapiens

text: female

text: breast carcinoma in situ

text: plasmid (ampicillin resistant)


text: live

text: Generic

text: mRNA made from breast epithelium, cDNA made by oligo-dT priming. Directionally cloned. Size-selected on agarose gel, average insert size 500 bp. Primary library, non-amplified.

text: adult

characteristic: lab-host

text: breast

text: Homo sapiens

text: female

text: normal epithelium

text: plasmid (ampicillin resistant)

characteristic: vector


characteristic: External Id

text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.33 kb. Tumor types include: meningioma, oligodendroglioma, astrocytoma (grade II), medulloblastoma, astrocytoma (grade IV). Life Technologies catalog #: 11544-012

text: brain

text: Homo sapiens

characteristic: organism

text: SalI

text: NotI

text: tumor, 5 pooled (see description)

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.7 kb. Life Technologies catalog #: 11531-019

text: colon

text: Homo sapiens

text: SalI

text: NotI

text: moderately-differentiated adenocarcinoma

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.1 kb. Life Technologies catalog #: 11502-010

text: esophagus

text: Homo sapiens

text: SalI

text: NotI

text: squamous cell carcinoma

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.69 kb. Life Technologies catalog #: 11549-011

text: stomach

text: Homo sapiens

text: SalI

text: NotI

text: poorly differentiated adenocarcinoma with signet ring cell features

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.2 kb. Life Technologies catalog #: 11524-014

text: kidney

text: Homo sapiens

text: SalI

text: NotI

text: renal cell tumor

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.25 kb. Life Technologies catalog #: 11547-015

text: lymph node

text: Homo sapiens

text: SalI

text: NotI

text: lymphoma, follicular mixed small and large cell

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.2 kb. Non-amplified library. ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'

text: SOLR (Stratagene, kanamycin resistant)

text: lymph node

text: Homo sapiens

text: EcoRI

text: XhoI

text: follicular lymphoma

text: phagemid (ampicillin resistant)

text: pBluescript SK-


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 0.8 kb. Non-amplified library. ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'

text: SOLR (Stratagene, kanamycin resistant)

text: lymph node

text: Homo sapiens

text: EcoRI

text: XhoI

text: mantle cell lymphoma

text: phagemid (ampicillin resistant)

text: pBluescript SK-


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.35 kb. Tumor types include: mixed Mullerian tumor, papillary serous, clear cell, spindle cell. All are primary tumors, metastasis positive. Life Technologies catalog #: 11534-013

text: ovary

text: Homo sapiens

text: SalI

text: NotI

text: tumor, 5 pooled (see description)

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: mRNA made from ovarian carcinoma, cDNA made by oligo-dT priming. Non-directionally cloned. Size-selected on agarose gel, average insert size 500 bp. Non-amplified library.

text: ovary

text: Homo sapiens

text: female

characteristic: sex

text: papillary serous carcinoma

text: plasmid (ampicillin resistant)


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

characteristic: INSDC center name

text: live

text: Generic

text: mRNA made from borderline ovarian carcinoma, cDNA made by oligo-dT priming. Directionally cloned. Size-selected on agarose gel, average insert size 500 bp. Primary library, non-amplified.

text: adult

text: ovary

text: Homo sapiens

text: female

text: borderline ovarian carcinoma

text: plasmid (ampicillin resistant)


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.72 kb. Life Technologies catalog #: 11548-013

text: pancreas

characteristic: organ

text: Homo sapiens

text: SalI

text: NotI

text: adenocarcinoma

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.75 kb. Life Technologies catalog #: 11538-014

text: uterus

text: Homo sapiens

text: SalI

text: NotI

text: well-differentiated endometrial adenocarcinoma, 7 pooled tumors

characteristic: title

text: phagemid (ampicillin resistant)


characteristic: INSDC first public

text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.85 kb. Life Technologies catalog #: 11539-012

text: uterus

text: Homo sapiens

text: SalI

text: NotI

text: moderately-differentiated endometrial adenocarcinoma, 3 pooled tumors

text: phagemid (ampicillin resistant)


characteristic: INSDC last update

text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.45 kb. Life Technologies catalog #: 11541-018

text: uterus

text: Homo sapiens

text: SalI

text: NotI

text: poorly-differentiated endometrial adenocarcinoma, 2 pooled tumors

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.48 kb. Life Technologies catalog #: 11542-016

text: uterus

text: Homo sapiens

text: SalI

text: NotI

text: serous papillary carcinoma, high grade, 2 pooled tumors

text: phagemid (ampicillin resistant)


text: Justus-Liebig-University

text: live

text: Generic

text: hemocyte

characteristic: cell type

text: fifth larval instar

text: Manduca sexta

text: tobacco hornworm induced hemocyte library


text: The Rockefeller University

text: live

characteristic: INSDC status

text: Generic

text: Bright Yellow 2

characteristic: cultivar

text: identified by differential display

text: Nicotiana tabacum

text: Tobacco mRNA/differential display (Horvath et al.)


text: The Institute of Physical and Chemical Research (RIKEN)

text: live

text: Generic

characteristic: NCBI submission model

text: Arabidopsis thaliana

text: YAC clone CIC7E11 region-specific cDNA


text: live

text: Generic

characteristic: NCBI submission package

text: Arabidopsis thaliana

text: YAC clone CIC8B11 region-specific cDNA


text: National Institute of Infectious Diseases

text: live

text: Generic

text: 1st strand cDNA was primed with an oligo(dT) primer ATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded cDNA was ligated to a DraIII adaptor [TGTTGGCCTACTGG], digested and cloned into distinct DraIII sites of the pME18S-FL3. XhoI sites just outside the DraIII sites can be used to isolate the cDNA insert. Size selection was performed to exclude fragments <1.5 kb. Library was constructed by Sugano et al.(University of Tokyo, Institute of Medical Science). Custom primer used for sequencing: 5' end primer [CTTCTGCTCTAAAAGCTGCG], 3' end primer [CGACCTGCAGCTCGAGCACA]

characteristic: description

text: adult

text: brain

text: Mus musculus

text: female

characteristic: strain

text: Sugano mouse brain mncb


text: Yamaguchi University

text: live

text: Generic

text: Brassica rapa

text: root

characteristic: tissue-type

text: Chinese cabbage root cDNA


text: College of Natural Sciences, Kyungpook National University

text: live

text: Generic

text: 6 - 48 hours post fertilization

characteristic: dev-stage

text: Danio rerio

text: male and female mix

text: embryonic

text: Zebrafish cDNA Library


characteristic: External Id

text: Univ. of Vienna

text: live

text: Generic

characteristic: cell-line

text: A subclone derived from A431 (ATCC) overexpressing the small heat shock protein hsp27

text: Homo sapiens

text: Human cell line A431 subclone


text: Institute for Medical Microbiology

text: live

text: Generic

text: Mus musculus

characteristic: organism

text: Mouse cell line V2D1 mRNA


text: Seoul National University

characteristic: INSDC center name

text: live

text: Generic

text: Griffithsia japonica

text: Griffithsia japonica mRNA


text: live

text: Generic

text: Total RNA (purified with Trizol and DNAsed before use) was reverse transcribed using a modified oligo-dT primer containing RsaI and HindIII sites. Double- stranded cDNA was digested with RsaI, resulting in blunt ended cDNA of an average 0.1-2 kb in length. Digested cDNA was split into two sets, one used as is as the driver, the other set was split in half again and each half linked to a different adaptor (5'-TCGAGCGGCCGCCCGGGCAGGT-3' or 5'- AGGGCGTGGTGCGGAGGGCGGT-3'), to be used as tester. Subtraction was performed using the Clontech PCR Select cDNA subtraction kit. Pool of two male bipolars, ages 34 and 48 (S-75, S-83) subtracted by pool of two mentally normal individuals, male, age 48 and female, age 35 (S-65, S-136). Tissues were obtained from the Stanley Neuropathology Consortium (www.stanleylab.org). Library constructed and subtracted by Dr. Nancy Johnston [(410) 614-3918, nlj@welchlink.welch.jhu.edu].

text: DH10B (phage-resistant)

characteristic: lab-host

text: brain

characteristic: organ

text: Homo sapiens

text: EcoRI

text: frontal lobe (see description)

text: Stanley Frontal BN pool 1

text: plasmid (ampicillin resistant)

text: pCR2.1-TOPO (Invitrogen)

characteristic: vector


text: Washington University School of Medicine

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Total RNA (purified with Trizol and DNAsed before use) was reverse transcribed using a modified oligo-dT primer containing RsaI and HindIII sites. Double- stranded cDNA was digested with RsaI, resulting in blunt ended cDNA of an average 0.1-2 kb in length. Digested cDNA was split into two sets, one used as is as the driver, the other set was split in half again and each half linked to a different adaptor (5'-TCGAGCGGCCGCCCGGGCAGGT-3' or 5'- AGGGCGTGGTGCGGAGGGCGGT-3'), to be used as tester. Subtraction was performed using the Clontech PCR Select cDNA subtraction kit. Pool of two mentally normal males, ages 41 and 53 (S-124, S-141) subtracted by pool of two bipolar males, ages 45 and 50 (S-111, S-128). Tissues were obtained from the Stanley Neuropathology Consortium (www.stanleylab.org). Library constructed and subtracted by Dr. Nancy Johnston [(410) 614-3918, nlj@welchlink.welch.jhu.edu].

text: DH10B (phage-resistant)

text: brain

text: Homo sapiens

text: EcoRI

text: frontal lobe (see description)

text: Stanley Frontal NB pool 2

characteristic: title

text: plasmid (ampicillin resistant)

text: pCR2.1-TOPO (Invitrogen)


text: live

characteristic: INSDC status

text: Generic

text: Total RNA (purified with Trizol and DNAsed before use) was reverse transcribed using a modified oligo-dT primer containing RsaI and HindIII sites. Double- stranded cDNA was digested with RsaI, resulting in blunt ended cDNA of an average 0.1-2 kb in length. Digested cDNA was split into two sets, one used as is as the driver, the other set was split in half again and each half linked to a different adaptor (5'-TCGAGCGGCCGCCCGGGCAGGT-3' or 5'- AGGGCGTGGTGCGGAGGGCGGT-3'), to be used as tester. Subtraction was performed using the Clontech PCR Select cDNA subtraction kit. Pool of two mentally normal male individuals ages 41 and 53 (S-124, S-141) subtracted by pool of two schizophrenic individuals, male age 44 and female age 56 (S-116, S-118). Tissues were obtained from the Stanley Neuropathology Consortium (www.stanleylab.org). Library constructed and subtracted by Dr. Nancy Johnston [(410) 614-3918, nlj@welchlink.welch.jhu.edu].

text: DH10B (phage-resistant)

text: brain

text: Homo sapiens

text: EcoRI

text: frontal lobe (see description)

text: Stanley Frontal NS pool 2

text: plasmid (ampicillin resistant)

text: pCR2.1-TOPO (Invitrogen)


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

characteristic: NCBI submission model

text: Total RNA (purified with Trizol and DNAsed before use) was reverse transcribed using a modified oligo-dT primer containing RsaI and HindIII sites. Double- stranded cDNA was digested with RsaI, resulting in blunt ended cDNA of an average 0.1-2 kb in length. Digested cDNA was split into two sets, one used as is as the driver, the other set was split in half again and each half linked to a different adaptor (5'-TCGAGCGGCCGCCCGGGCAGGT-3' or 5'- AGGGCGTGGTGCGGAGGGCGGT-3'), to be used as tester. Subtraction was performed using the Clontech PCR Select cDNA subtraction kit. Pool of two male schizophrenics, ages 30 and 32 (S-30, S-121) subtracted by pool of two male bipolars, age 34 and 48 (S-75, S-83). Tissues were obtained from the Stanley Neuropathology Consortium (www.stanleylab.org). Library constructed and subtracted by Dr. Nancy Johnston [(410) 614-3918, nlj@welchlink.welch.jhu.edu].

text: DH10B (phage-resistant)

text: brain

text: Homo sapiens

text: EcoRI

text: frontal lobe (see description)

text: Stanley Frontal SB pool 1

text: plasmid (ampicillin resistant)

text: pCR2.1-TOPO (Invitrogen)


text: live

text: Generic

characteristic: NCBI submission package

text: Total RNA (purified with Trizol and DNAsed before use) was reverse transcribed using a modified oligo-dT primer containing RsaI and HindIII sites. Double- stranded cDNA was digested with RsaI, resulting in blunt ended cDNA of an average 0.1-2 kb in length. Digested cDNA was split into two sets, one used as is as the driver, the other set was split in half again and each half linked to a different adaptor (5'-TCGAGCGGCCGCCCGGGCAGGT-3' or 5'- AGGGCGTGGTGCGGAGGGCGGT-3'), to be used as tester. Subtraction was performed using the Clontech PCR Select cDNA subtraction kit. 34 yo schizophrenic male (S-11) subtracted by 28 yo mentally normal male (S-37). Tissues were obtained from the Stanley Neuropathology Consortium (www.stanleylab.org). Library constructed and subtracted by Dr. Nancy Johnston [(410) 614-3918, nlj@welchlink.welch.jhu.edu].

text: DH10B (phage-resistant)

text: brain

text: Homo sapiens

text: EcoRI

text: XhoI

text: frontal lobe (see description)

text: Stanley Frontal SN individual

text: plasmid (ampicillin resistant)

text: pCR2.1 (Invitrogen)


text: live

text: Generic

text: Total RNA (purified with Trizol and DNAsed before use) was reverse transcribed using a modified oligo-dT primer containing RsaI and HindIII sites. Double- stranded cDNA was digested with RsaI, resulting in blunt ended cDNA of an average 0.1-2 kb in length. Digested cDNA was split into two sets, one used as is as the driver, the other set was split in half again and each half linked to a different adaptor (5'-TCGAGCGGCCGCCCGGGCAGGT-3' or 5'- AGGGCGTGGTGCGGAGGGCGGT-3'), to be used as tester. Subtraction was performed using the Clontech PCR Select cDNA subtraction kit. Pool of two male schizophrenics, ages 30 and 32 (S-30, S-121) subtracted by pool of two mentally normal individuals, male, age 48 and female, age 35 (S-65, S-136). Tissues were obtained from the Stanley Neuropathology Consortium (www.stanleylab.org). Library constructed and subtracted by Dr. Nancy Johnston [(410) 614-3918, nlj@welchlink.welch.jhu.edu].

characteristic: description

text: DH10B (phage-resistant)

text: brain

text: Homo sapiens

text: EcoRI

text: frontal lobe (see description)

text: Stanley Frontal SN pool 1

text: plasmid (ampicillin resistant)

text: pCR2.1-TOPO (Invitrogen)


text: live

text: Generic

text: Total RNA (purified with Trizol and DNAsed before use) was reverse transcribed using a modified oligo-dT primer containing RsaI and HindIII sites. Double- stranded cDNA was digested with RsaI, resulting in blunt ended cDNA of an average 0.1-2 kb in length. Digested cDNA was split into two sets, one used as is as the driver, the other set was split in half again and each half linked to a different adaptor (5'-TCGAGCGGCCGCCCGGGCAGGT-3' or 5'- AGGGCGTGGTGCGGAGGGCGGT-3'), to be used as tester. Subtraction was performed using the Clontech PCR Select cDNA subtraction kit. Pool of two schizophrenics, male age 44 and female age 56 (S-116, S-118) subtracted by pool of two mentally normal male individuals ages 41 and 53 (S-124, S-141). Tissues were obtained from the Stanley Neuropathology Consortium (www.stanleylab.org). Library constructed and subtracted by Dr. Nancy Johnston [(410) 614-3918, nlj@welchlink.welch.jhu.edu].

text: DH10B (phage-resistant)

text: brain

text: Homo sapiens

text: EcoRI

text: frontal lobe (see description)

text: Stanley Frontal SN pool 2

text: plasmid (ampicillin resistant)

text: pCR2.1-TOPO (Invitrogen)


text: live

text: Generic

text: Total RNA (purified with Trizol and DNAsed before use) was reverse transcribed using a modified oligo-dT primer containing RsaI and HindIII sites. Double- stranded cDNA was digested with RsaI, resulting in blunt ended cDNA of an average 0.1-2 kb in length. Digested cDNA was split into two sets, one used as is as the driver, the other set was split in half again and each half linked to a different adaptor (5'-TCGAGCGGCCGCCCGGGCAGGT-3' or 5'- AGGGCGTGGTGCGGAGGGCGGT-3'), to be used as tester. Subtraction was performed using the Clontech PCR Select cDNA subtraction kit. Pool of three bipolar individuals, male, ages 48 and 50, female, age 37 (S-47, S-83, S-128) subtracted by pool of three mentally normal individuals, male, ages 34 and 48, female, age 50 (S-65, S-67, S-164). Tissues were obtained from the Stanley Neuropathology Consortium (www.stanleylab.org). Library constructed and subtracted by Dr. Nancy Johnston [(410) 614-3918, nlj@welchlink.welch.jhu.edu].

text: DH10B (phage-resistant)

text: brain

text: Homo sapiens

text: EcoRI

text: hippocampus (see description)

text: Stanley Hippocampus BN pool 1

text: plasmid (ampicillin resistant)

text: pCR2.1-TOPO (Invitrogen)


text: live

text: Generic

text: Total RNA (purified with Trizol and DNAsed before use) was reverse transcribed using a modified oligo-dT primer containing RsaI and HindIII sites. Double- stranded cDNA was digested with RsaI, resulting in blunt ended cDNA of an average 0.1-2 kb in length. Digested cDNA was split into two sets, one used as is as the driver, the other set was split in half again and each half linked to a different adaptor (5'-TCGAGCGGCCGCCCGGGCAGGT-3' or 5'- AGGGCGTGGTGCGGAGGGCGGT-3'), to be used as tester. Subtraction was performed using the Clontech PCR Select cDNA subtraction kit. Pool of three mentally normal individuals, male, ages 34 and 48, female, age 50 (S-65, S-67, S-164) subtracted by pool of three bipolar individuals, male, ages 48 and 50, female, age 37 (S-47, S-83, S-128). Tissues were obtained from the Stanley Neuropathology Consortium (www.stanleylab.org). Library constructed and subtracted by Dr. Nancy Johnston [(410) 614-3918, nlj@welchlink.welch.jhu.edu].

text: DH10B (phage-resistant)

text: brain

text: Homo sapiens

text: EcoRI

text: hippocampus (see description)

text: Stanley Hippocampus NB pool 1

text: plasmid (ampicillin resistant)

text: pCR2.1-TOPO (Invitrogen)


text: live

text: Generic

text: Total RNA (purified with Trizol and DNAsed before use) was reverse transcribed using a modified oligo-dT primer containing RsaI and HindIII sites. Double- stranded cDNA was digested with RsaI, resulting in blunt ended cDNA of an average 0.1-2 kb in length. Digested cDNA was split into two sets, one used as is as the driver, the other set was split in half again and each half linked to a different adaptor (5'-TCGAGCGGCCGCCCGGGCAGGT-3' or 5'- AGGGCGTGGTGCGGAGGGCGGT-3'), to be used as tester. Subtraction was performed using the Clontech PCR Select cDNA subtraction kit. Pool of three schizophrenic males, ages 34, 58 and 60 (S-11, S-64, S-109) subtracted by pool of three bipolar individuals, male, ages 48 and 50, female, age 37 (S-47, S-83, S-128). Tissues were obtained from the Stanley Neuropathology Consortium (www.stanleylab.org). Library constructed and subtracted by Dr. Nancy Johnston [(410) 614-3918, nlj@welchlink.welch.jhu.edu].

text: DH10B (phage-resistant)

text: brain

text: Homo sapiens

text: EcoRI

text: hippocampus (see description)

text: Stanley Hippocampus SB pool 1

text: plasmid (ampicillin resistant)

text: pCR2.1-TOPO (Invitrogen)


text: live

text: Generic

text: Total RNA (purified with Trizol and DNAsed before use) was reverse transcribed using a modified oligo-dT primer containing RsaI and HindIII sites. Double- stranded cDNA was digested with RsaI, resulting in blunt ended cDNA of an average 0.1-2 kb in length. Digested cDNA was split into two sets, one used as is as the driver, the other set was split in half again and each half linked to a different adaptor (5'-TCGAGCGGCCGCCCGGGCAGGT-3' or 5'- AGGGCGTGGTGCGGAGGGCGGT-3'), to be used as tester. Subtraction was performed using the Clontech PCR Select cDNA subtraction kit. Pool of three schizophrenic males, ages 34, 58 and 60 (S-11, S-64, S-109) subtracted by pool of three mentally normal individuals, male, ages 34 and 48, female, age 50 (S-65, S-67, S-164). Tissues were obtained from the Stanley Neuropathology Consortium (www.stanleylab.org). Library constructed and subtracted by Dr. Nancy Johnston [(410) 614-3918, nlj@welchlink.welch.jhu.edu].

text: DH10B (phage-resistant)

text: brain

text: Homo sapiens

text: EcoRI

text: hippocampus (see description)

text: Stanley Hippocampus SN pool 1

text: plasmid (ampicillin resistant)

text: pCR2.1-TOPO (Invitrogen)


text: Anatomy and Cellular Biology

text: live

text: Generic

text: E8CASS; variant of MCF7

text: Homo sapiens

text: breast adenocarcinoma

text: Human E8CASS


text: Heidelberg University

text: live

text: Generic

text: Trypanosoma brucei

characteristic: strain

text: Trypanosoma brucei strain 927 ESTs


text: UIC College of Medicine

text: live

text: Generic

text: The S.mansoni schistosomula cDNA library was obtained from Charles B. Shoemaker, Harvard School of Public Health, Boston, MA, USA. The library was constructed in Uni-ZAP XR vector (stratagene). The original library contained 1 X 10E6 recombinants and the amplified library had a titer of 6 X 10E9 pfu/ml.

text: XL-1 Blue MRF'

text: Schistosoma mansoni

text: EcoRI

text: XhoI

text: S.mansoni Schistosomula

text: Phage

text: Uni-ZAP XR


text: Max-Planck-Institut fuer Biochemie

text: live

text: Generic

text: epithelial

characteristic: cell type

text: 8a (transformed, differentiated F9 cells)

text: PCR (RACE)

text: Mus musculus

text: Mouse 8a cell line (I.Oberbaeumer)


text: University of Georgia

text: live

text: Generic

text: Bos taurus

text: pituitary

characteristic: tissue-type

text: Bos taurus pituitary mRNA library


characteristic: External Id

text: Faculte de Medecine Henri Warembourg

text: live

text: Generic

text: HeLa

text: isolated from retinoid treated cells

text: Homo sapiens

text: Retinoid treated HeLa cells


text: Kumho Life & Environmental Science Laboratory (KLESL)

text: live

text: Generic

text: The plasmid vector, pSMASH, is a yeast expression vector derived from pGAD424 (Clontech). It was designed for selective cloning of the genes encoding secreted and membrane proteins. Briefly, it contains a EcoRI-NotI subcloning sites followed by a Kex2 protease recognition site and invertase lacking its own methionine and signal sequence.

text: seedling

characteristic: dev-stage

text: Arabidopsis thaliana

text: EcoRI

text: NotI

text: whole plant

text: Arabidopsis pSMASH Library

text: plasmid

text: pSMASH


text: Beijing Medical University

text: live

text: Generic

text: a systemic lupus erythematosus (SLE) model mouse

text: Mus musculus

characteristic: organism

text: BXSB

text: Mouse strain BXSB mRNA


characteristic: INSDC center name

text: live

text: Generic

text: Plasmid DNA from the normalized library NCI_CGAP_Kid3 was prepared, and ss circles were made in vitro. Following HAP purification, this DNA was used as tracer in a subtractive hybridization reaction. The driver was PCR-amplified cDNAs from a pool of 5,000 clones made from the same library (cloneIDs 1322376-1323911, 1456007-1456775, and 1500552-1502855). Subtraction by Bento Soares and M. Fatima Bonaldo.

text: kidney

text: Homo sapiens

text: Not I

text: Eco RI

text: phagemid (ampicillin resistant)


text: Saitama Medical School

text: live

text: Generic

text: Mus musculus

text: brain

text: Mus musculus brain mRNA (A.Orimo)


text: IBVM, INRA-CR Bordeaux

text: live

text: Generic

text: cloned by DDRT-PCR

text: 3 days after pollination

text: carpel

text: Silene latifolia

text: female

characteristic: sex

text: wild type

text: pericarp

text: Silene latifolia pGEMCF Library

text: Plasmid

text: pGEM-T (Promega)


text: Shanghai Institute of Biochemistry, Chinese Academy of Sciences

text: live

text: Generic

text: directionally cloned in a eukaryotic expression vector pCMV.SPORT; GIBCO BRL

characteristic: lab-host

text: Rattus norvegicus

text: male

text: Sprague-Dawley

text: brain

text: SuperScript Rat Brain cDNA Library

text: pCMV.SPORT


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: Gel-purification of HPL-RB4 to isolate clones with larger insert size. Library constructed by Bob Barstead.

text: adult, age 89

text: prostate

characteristic: organ

text: Homo sapiens

text: EcoRI

text: NotI

text: male

text: Barstead prostate BPH HPLRB4 1

text: phagemid (ampicillin resistant)

characteristic: vector


text: CSIRO Tropical Agriculture

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: The cephalothorax content of 5 adult shrimp (sex not determined) was used in the RNA preparation for this library. This includes most organ and tissue types of shrimp: nervous system, digestive system, endocrine system, heart, gills, eyestalk, epidermis, gonads etc. mRNA isolated from cephalothorax tissue was used to construct a unidirectional library in UniZap. Clones excised in vivo to create phagemids in pBluescript SK- for sequencing.

text: adult

text: SOLR

text: Penaeus monodon

text: EcoRI

text: XhoI

text: most tissue types of shrimp

text: Black Tiger Shrimp Whole Cephalothorax UniZap library

text: phage

text: UNiZap


text: Australian Institute of Marine Science

text: live

characteristic: INSDC status

text: Generic

text: mRNA isolated from total eyestalk tissue and used to construct directional cDNA library in Lambda ZAP Express. Clones excised in vivo to create phagemids in pBK-CMV for sequencing.

text: Mature broodstock

text: Penaeus monodon

text: EcoRI

text: XhoI

text: Female

text: Eyestalk

text: Giant tiger prawn eyestalk cDNA library

characteristic: title

text: Phage

text: Lambda ZAP Express


text: Australian Institute of Marine Science

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: mRNA isolated from total pleopod tissue and used to construct directional cDNA library in Lambda ZAP Express. Clones excised in vivo to create phagemids in pBK-CMV for sequencing. This library contains a high proportion of mitochondrial sequences.

text: Mature broodstock

text: Penaeus monodon

text: EcoRI

text: XhoI

text: Male

text: Pleopods (swimming legs)

text: Giant tiger prawn pleopod cDNA library

text: Phage

text: Lambda ZAP Express


text: International Center of Tropical Agriculture (CIAT)

text: live

text: Generic

text: TMS 30572; CM2177-2

characteristic: cultivar

text: DH5a E.coli

text: Manihot esculenta

text: Leaf and roots

text: Cassava leaf and root cDNA libraries


text: LMOD

text: live

text: Generic

text: Rattus norvegicus

text: Wistar

characteristic: strain

text: whole lens

text: Rattus norvegicus whole lens mRNA


text: Cancer Research Institute

text: live

text: Generic

characteristic: cell-line

text: Homo sapiens

text: nasopharyngeal carcinoma

characteristic: tissue-type

text: Human nasopharyngeal carcinoma cell line HNE1


characteristic: External Id

text: Martin Luther University Halle-Wittenberg, Medical Faculty

text: live

text: Generic

text: four-day-old and six-day-old preimplantation embryo

characteristic: dev-stage

text: Oryctolagus cuniculus

text: hybrid strain Zika

text: Rabbit 4-day-old and 6-day-old embryo


characteristic: INSDC center name

text: live

text: Generic

text: four-day-old preimplantation embryo

text: Oryctolagus cuniculus

characteristic: organism

text: hybrid strain Zika

text: Rabbit 4-day-old embryo


text: Lawrence Berkeley National Lab

text: live

text: Generic

text: Sized fractionated cDNAs were directly ligated into pOT2. Plasmid cDNA library.

characteristic: description

text: larvae-pupae

characteristic: lab-host

text: whole body

text: Drosophila melanogaster

text: EcoRI

text: male and female

characteristic: sex

text: LP Drosophila melanogaster larval-early pupal pOT2


text: U78 INSERM

characteristic: INSDC first public

text: live

text: Generic

text: adult

text: Rattus norvegicus

text: Sprague-Dawley

text: Rat ESTs (E.Olivier)

text: phage

text: lambda ZAPII

characteristic: vector


text: The Institute of Physical and Chemical Research

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Arabidopsis thaliana

text: Columbia


text: Cambridge Institute for Medical Research

text: live

text: Generic

characteristic: NCBI submission model

text: Leishmania major

text: Friedlin

text: Leishmania major Amastigote full length cDNA library

characteristic: title


text: Washington University School of MedicineP

text: live

text: Generic

characteristic: NCBI submission package

text: colon cancer cell line

text: Double-stranded cDNA was prepared from cell line MAC16 using primer 5'-GAGAGAGAGAGAGAGAGAGAAACTAGTCTGAGT(18)-3'. An EcoRI adaptor was used on the 5' end of the cDNA as follows: 5'-AATTCGGCACGAG-3'. The library was size-selected and went through one round of amplification. Average insert size is 1.7 kb, with a range from 0.4-12 kb. This library was constructed by Dr. Martin Schiller (Johns Hopkins University).

text: SOLR

text: Mus musculus

text: EcoRI

text: XhoI

text: Schiller mouse MAC16

text: plasmid (ampicillin resistant)

text: pBluescript SK- (Stratagene)


text: Louisiana State University, Medical Center

text: live

text: Generic

text: Corneal epithelial cell

characteristic: cell type

text: adult

text: Oryctolagus cuniculus

text: female

text: Corneal epithelium

text: Rabbit corneal epithelium cDNA library


text: Jawaharlal Nehru University

text: live

text: Generic

text: Labeo rohita

text: liver

text: Labeo rohita liver


text: Department for Biochemistry

text: live

text: Generic

text: Gigaspora rosea

text: nongerminated spores

text: Gigaspora rosea nongerminated spores


text: New York University Medical Center

text: live

text: Generic

text: leukemic erythrocyte

text: normalized K562

characteristic: cell-line

text: Homo sapiens

text: Homo sapiens normalized K562


text: Georgetown University, Medical Center

text: live

text: Generic

text: from RTPCR differential display experiment

text: Candida albicans

characteristic: strain

text: Candida albicans SC5314


characteristic: External Id

text: University of Washington

text: live

text: Generic

text: Acetabularia acetabulum

text: Acetabularia acetabulum mRNA library


text: University of Tuebingen, Med Clinic 4

text: live

text: Generic

text: Sus scrofa domesticus

text: thyroid

characteristic: tissue-type

text: Sus scrofa domestica thyroid


text: Skirball Institute, New York University School of Medicine

characteristic: INSDC center name

text: live

text: Generic

text: Library was constructed by Bruce Appel and Judith Eisen in Stratagene Uni-Zap XR lambda vector with Zap-cDNA synthesis kit: EcoRI linkers were added to 5' ends and XhoI linkers were added to 3' ends and resulting cDNAs were ligated into Uni-Zap XR lambda vector. In vivo mass excision was used to generate cDNAs in pBluescript SK.

text: Danio rerio

text: EcoRI

text: XhoI

text: mixed

text: pooled 15-19 hr zebrafish embryos

text: Appel-Eisen zebrafish embryo 15-19 hr

text: pBluescript SK (-)


text: Washington University School of Medicine

text: live

text: Generic

text: 1st strand cDNA primed with (GA)10ACTAGTCTCGAG(T)18, followed by second strand synthesis, and ligated to 5' adapter (5')-aattcggcacgag- 3', 3'-gccgtgctc-5'. cDNA was cloned directionally (EcoRI/XhoI) into Stratagene Zap express lambda phage arms. Mass invivo excision done to obtain inserts in pBK-CMV phagemid.

text: E. coli XLOLR

text: Danio rerio

characteristic: organism

text: EcoRI

text: XhoI

text: mixed male and female

text: 0 day fin regenerates

text: zebrafish fin day0 regeneration

text: phagemid

text: pBK-CMV


text: live

text: Generic

characteristic: description

text: E. coli XLOLR

text: Danio rerio

text: EcoRI

text: XhoI

text: mixed male and female

text: 1 day fin regenerates

text: zebrafish fin day1 regeneration

text: phagemid

text: pBK-CMV


text: Lawrence Livermore National Laboratory

text: live

text: Generic

text: Original library was constructed in lambdaZIPLOX. Mass excision of the cDNA library was performed to yield pZIPLOX plasmids. Insert check was done in original library.

text: adult

characteristic: dev-stage

text: E. coli DH10B

characteristic: lab-host

text: Danio rerio

text: NotI

text: SalI

text: mixed male and female

text: brain

text: zebrafish adult brain

text: phagemid

text: pZIPLOX


characteristic: INSDC first public

text: live

text: Generic

text: 1st strand cDNA was primed with a Not I - oligo(dT)15 primer [5'pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT3']; double-stranded cDNA was ligated to Sal I adaptors (BRL), digested with Not I and cloned into the Not I and Sal I sites of the pSPORT1 vector (BRL). Library was constructed by Matthew Clark (Lehrach lab; ICRF, London and Max Planck Institut fuer Molekulare Genetik,Berlin). cDNAs for EST analysis were selected following oligonucleotide hybridization fingerprinting of arrayed clones from zebrafish late somitogenesis (26 ss), adult liver or embryonic shield stage (5.6 h) libraries. Fingerprint data were used to computationally cluster cDNAs, and a single cDNA from each cluster was chosen for sequencing. In some cases multiple members of the same cluster were sequenced to assess clustering parameters or single clones were sequenced additional times to assess quality control.

text: XL1-blue MRF

text: Danio rerio

text: NotI

text: SalI

text: mixed

characteristic: sex

text: 26 somite embryos, adult livers, shield stage embryos

text: Zebrafish WashU MPIMG EST

text: phagemid


text: Japan Science and Technology Corporation (JST)

characteristic: INSDC last update

text: live

text: Generic

text: four-cell-embryo

text: Mus musculus

text: Mouse four-cell-embryo cDNA


text: National Agricultural Science and Technology Institute

text: live

characteristic: INSDC status

text: Generic

text: average insert size: 1.0 Kb; initial pfu:1.12 X 10 6

text: XL1-Blue MRF and SOLAR

text: Bombyx mandarina

text: EcoRI

text: XhoI

text: whole body

text: Bombyx mandarina library (Hwang JS)


text: Faculty of Veterinary Medicine, Utrecht University

text: live

text: Generic

characteristic: NCBI submission model

text: Cricetinae gen. sp.

text: Cricetinae gen. sp. from hamster-human somatic cell-hybrid Q1Z


text: live

text: Generic

text: Homo sapiens

text: Homo sapiens from hamster-human somatic cell-hybrid Q1Z

characteristic: title


text: Guys Hospital

text: live

text: Generic

characteristic: NCBI submission package

text: fetus

text: Homo sapiens

text: brain

text: Homo sapiens brain fetus


text: Auburn University

text: live

text: Generic

text: adult

text: Ictalurus punctatus

text: female

text: Kansas

text: pituitary

text: Channel catfish pituitary library


text: United States Department of Agriculture-Agriculture Research Service

text: live

text: Generic

text: Clontech PCR Select protocol. Pyrethroid and Diazinon resistant wild type horn flies from April 1997 collection at Camp Cooley subtracted with Kerrville citrated blood-fed lab susceptible strain unfed newly emerged adults.

text: adults of mixed ages

text: Haematobia irritans irritans

text: Mixed male and female

text: Camp Cooley 4/97 Wild Type

text: whole fly

text: Horn Fly PCR Select Subtracted Camp Cooley Library


text: live

text: Generic

text: sixteen-cell-embryo

text: Mus musculus

text: Mouse sixteen-cell-embryo cDNA


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: mRNA made from breast carcinoma tissue, cDNA made by oligo-dT priming. Directionally cloned. Size-selected on agarose gel, average insert size 400 bp. Primary library, non-amplified.

text: adult

text: breast

characteristic: organ

text: Homo sapiens

text: female

text: lobullar carcinoma in situ

text: plasmid (ampicillin resistant)

characteristic: vector


text: live

text: Generic

text: mRNA made from breast adenocarcinoma tissue, cDNA made by oligo-dT priming. Directionally cloned. Size-selected on agarose gel, average insert size 400 bp. Primary library, non-amplified.

text: adult

text: breast

text: Homo sapiens

text: female

text: adenocarcinoma

text: plasmid (ampicillin resistant)


text: live

text: Generic

text: mRNA made from oligodendroglioma tissue, cDNA made by oligo-dT priming. Directionally cloned. Size-selected on agarose gel, average insert size 500 bp. Primary library, non-amplified.

text: adult

text: brain

text: Homo sapiens

text: oligodendroglioma

text: plasmid (ampicillin resistant)


text: live

text: Generic

text: Plasmid DNA from the normalized library NCI_CGAP_Kid5 was prepared, and ss circles were made in vitro. Following HAP purification, this DNA was used as tracer in a subtractive hybridization reaction. The driver was PCR-amplified cDNAs from a pool of 5,000 clones made from the same library (cloneIDs 1323912-1325831, 1471368-1472903 and 1492104-1493255). Subtraction by Bento Soares and M. Fatima Bonaldo.

text: kidney

text: Homo sapiens

text: Not I

text: Eco RI

text: 2 pooled tumors (clear cell type)

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: mRNA made from bone marrow, stem cells 34+/38+, cDNA made by oligo-dT priming. Directionally cloned. Size-selected on agarose gel, average insert size 400 bp. Primary library, non-amplified.

text: adult

text: bone marrow

text: Homo sapiens

text: stem cell 34+/38+

text: plasmid (ampicillin resistant)


text: live

text: Generic

text: mRNA made from ovary carcinoma tissue, cDNA made by oligo-dT priming. Directionally cloned. Size-selected on agarose gel, average insert size 400 bp. Primary library, non-amplified.

text: adult

text: ovary

text: Homo sapiens

text: female

text: early stage papillary serous carcinoma

text: plasmid (ampicillin resistant)


text: live

text: Generic

text: Plasmid DNA from the normalized library NCI_CGAP_Co10 was prepared, and ss circles were made in vitro. Following HAP purification, this DNA was used as tracer in a subtractive hybridization reaction. The driver was PCR-amplified cDNAs from a pool of 5,000 clones made from the same library (cloneIDs 1057416-1061255, and 1144584-1145351). Subtraction by Bento Soares and M. Fatima Bonaldo.

text: colon

text: Homo sapiens

text: Not I

text: Eco RI

text: colon tumor, RER+

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: mRNA made from lung carcinoma tissue, cDNA made by oligo-dT priming. Directionally cloned. Size-selected on agarose gel, average insert size 500 bp. Primary library, non-amplified.

text: adult

text: lung

text: Homo sapiens

text: bronchioalveolar carcinoma

text: plasmid (ampicillin resistant)


text: live

text: Generic

text: mRNA made from lung adenocarcinoma tissue, cDNA made by oligo-dT priming. Directionally cloned. Size-selected on agarose gel, average insert size 500 bp. Primary library, non-amplified.

text: adult

text: lung

text: Homo sapiens

text: invasive adenocarcinoma

text: plasmid (ampicillin resistant)


text: NIH

text: live

text: Generic

text: Homo sapiens

text: brain

text: Homo sapiens brain (M.Centola et al.)


text: The University of Reading

text: live

text: Generic

text: 5-6 weeks post-germination

text: Zea mays

text: leaf

text: Zea mays leaf 5-6 weeks post-germination


text: National Agricultural Science and Technology Institute

text: live

text: Generic

text: Antheraea yamamai

text: posterior silkgland

text: posterior silkgland


text: University of Hanyang, Department of Life Science

text: live

text: Generic

text: embryonic stem cell

characteristic: cell type

text: Vector: Uni-Zap XR Phage and generate pBluescript-SK phagemid vector by in vivo excision.

text: blastocyst

text: Mus musculus

text: male

characteristic: strain

text: blastocyst inner cell mass

text: Mouse ES cell Lambda Zap Express Library

text: Uni-Zap XR


characteristic: External Id

text: Shanghai Institute of Biochemistry

text: live

text: Generic

text: Homo sapiens

text: laryngeal cancer

characteristic: tissue-type

text: Homo sapiens laryngeal cancer


text: Scottish Crop Research Institute

characteristic: INSDC center name

text: live

text: Generic

text: Stirling

characteristic: cultivar

text: Solanum tuberosum

characteristic: organism

text: Solanum tuberosum Stirling mRNA


text: Washington University School of Medicine

text: live

text: Generic

text: Brugia malayi


text: live

text: Generic

text: macrophage-like

text: PMA-induced HL60 human leukemic cell line

characteristic: cell-line

text: RNA from uninduced HL60 cells was subtracted against RNA from HL60 cells that were induced 8h with 16 nM PMA using the method of Wang and Brown (Proc Natl Acad Sci USA 88: 11505).

characteristic: description

characteristic: lab-host

text: Homo sapiens

text: EcoRI

text: EcoRI

text: PMA-induced HL60 cell subtraction library

text: plasmid

text: pSPORT 1


text: The Institute for Genomic Research

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Rattus norvegicus

text: EcoRI

text: NotI

text: PC12 cells, untreated, pT7T3Pac, TIGR


text: Korea University

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Hordeum vulgare

text: Hordeum vulgare library (Park YB)


text: Advanced Center for Genome Technology, University of Oklahoma

text: live

text: Generic

characteristic: NCBI submission package

text: See: Bell-Pedersen,D., et al. PNAS 93:13096,1996. 5' end of cDNA cloned into XbaI site of pBluescript; 3' end of cDNA cloned into EcoRI site of pBluescript

text: Neurospora crassa

text: XbaI

text: EcoRI

text: bd, frq7 A

text: tissue harvested following 22hr growth in dark

text: Neurospora crassa morning cDNA library

characteristic: title

text: phagemid

text: pBlueScript SK-


text: live

text: Generic

text: See: Bell-Perdersen,D., et al. PNAS 93:13096,1996. 5' end of cDNA cloned into XbaI site of pBluescript; 3' end of cDNA cloned into EcoRI site of pBluescript

text: Neurospora crassa

text: XbaI

text: EcoRI

text: Strain 30-7 (bd; A)

text: Neurospora crassa evening cDNA library

text: phagemid

text: pBlueScript SK-

characteristic: vector


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACGAATCTGAAGTGGGAGCGGCCGCCCTTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Eco RI adaptors [5' AATTCGGATCGAAC 3' and 5' GTTGGATCGG 3'], digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library constructed by Bob Barstead.

text: adult, age 64

characteristic: dev-stage

text: DH10B (phage resistant)

text: aorta

characteristic: organ

text: Homo sapiens

text: EcoRI

text: NotI

text: male

characteristic: sex

text: Barstead aorta HPLRB6

text: phagemid (ampicillin resistant)


characteristic: External Id

text: live

text: Generic

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACGAATCTGAAGTGGGAGCGGCCGCCCTTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Eco RI adaptors [5' AATTCACTAGTAAT 3' and 5' ATTACTAGTG 3'], digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library constructed by Bob Barstead.

text: adult, age 25

text: DH10B (phage resistant)

text: colon

text: Homo sapiens

text: EcoRI

text: NotI

text: male

text: Barstead colon HPLRB7

text: phagemid (ampicillin resistant)


characteristic: INSDC center name

text: live

text: Generic

text: mRNA made from developing eye tissue, cDNA made by oligo-dT priming with NotI oligo. SalI adaptor (5'-TCGACCCACGCGTCCG-3') ligated to 5' ends. Size-selected with cDNA size fractionation resin, average insert size 1.3 kb. Primary library, non-amplified. Library constructed by Dr. Pen Rashbass (penr@hgu.mrc.ac.uk).

text: embryo - 11.5 dpc

text: eye

text: Mus musculus

characteristic: strain

text: optic cup + lens vesicle

characteristic: tissue-type

text: Rashbass mouse MOC 11 5 optic cup

text: plasmid (ampicillin resistant)