text: Washington University School of Medicine

text: live

text: Generic

text: Williams

text: The cDNA library was constructed from mRNA isolated from the epicotyls of 2 week old seedling for the cultivar Williams. The seedlings were germinated in a growth chamber, excised above the soil level, and the plants were placed in a 100 ppm solution of auxin for 24 hours prior to harvesting. Complementary DNA was synthesized from mRNA using a primer consisting of a poly(dT) sequence with a XhoI restriction site. EcoRI adapters were ligated to the blunt-ended cDNA fragments followed by XhoI digestion. The cDNA fragments were directionally cloned into the EcoRI-XhoI restriction site of the pBluescript vector. The ligated cDNA fragments were transformed into DH10B host cells (GibcoBRL). This library was constructed in the laboratory of Dr. Randy Shoemaker.

text: 2 week old

text: Glycine max

text: EcoRI

text: XhoI

text: seedling epicotyls

characteristic: tissue-type

text: plasmid

text: pBluescript II SK+


status: PUBLIC

characteristic: External Id

text: Virginia Tech University

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: laticifer

tag: attribute

characteristic: cell type

text: unopened flower bud

characteristic: dev-stage

text: Papaver somniferum

characteristic: organism

text: Commercial variety

characteristic: strain

text: capsule latex

text: Opium Lambda Zap Express Library

characteristic: title


text: live

text: Generic

text: cDNA oligo dT-primed [5'-(GA)10-ACTAGTCTCGAGTTTTTTTTTTTTT-3'] and directionally cloned using 5' linkers 5'-AATTCGGCACGAG-3' and 5'-CTCGTGCCG-3'. Size selection of >400bp material gives average insert size ranging from 1-2 kb. Library was mass excised (from lambda-UniZAP-XR) and resulting single-stranded phagemids were prepped and tranformed into DH10B. Library contains 98.5% recombinants. References: J. Androl. 20:635-639 and Gene 25:263-269. Library constructed and donated by J. McCarrey, Ph.D. (Southwest Foundation for Biomedical Research, Dept. of Genetics); excision done by E.M. Eddy, Ph.D. (National Institutes of Health, National Institute of Environmental Health Sciences). Original lambda-based library is available through ATCC, catalog #63423.

characteristic: description

text: 60 day

text: DH10B (phage-resistant)

characteristic: lab-host

text: testis

characteristic: organ

text: Mus musculus

text: XhoII

text: EcoRI

text: male

characteristic: sex

text: round spermatids, pooled from multiple mice

text: McCarrey Eddy round spermatid

text: phagemid (ampicillin resistant)

text: pBluescript SK+ (Stratagene)

characteristic: vector


characteristic: External Id

text: live

text: Generic

text: cDNA oligo dT-primed [5'-(GA)10-ACTAGTCTCGAGTTTTTTTTTTTTT-3'] and directionally cloned using 5' linkers 5'-AATTCGGCACGAG-3' and 5'-CTCGTGCCG-3'. Size selection of >400bp material gives average insert size ranging from 1-2 kb. Library was mass excised (from lambda-UniZAP-XR) and resulting single-stranded phagemids were prepped and tranformed into DH10B. Library contains 98% recombinants. References: J. Androl. 20:635-639 and Gene 25:263-269. Library constructed and donated by J. McCarrey, Ph.D. (Southwest Foundation for Biomedical Research, Dept. of Genetics); excision done by E.M. Eddy, Ph.D. (National Institutes of Health, National Institute of Environmental Health Sciences). Original lambda-based library is available through ATCC, catalog #63422.

text: 60 day

text: DH10B (phage-resistant)

text: testis

text: Mus musculus

text: XhoI

text: EcoRI

text: male

text: spermatocytes, pooled from multiple mice

characteristic: tissue-type

text: McCarrey Eddy spermatocytes

text: phagemid (ampicillin resistant)

text: pBluescript SK+ (Stratagene)


characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: cDNA oligo dT-primed [5'-(GA)10-ACTAGTCTCGAGTTTTTTTTTTTTT-3'] and directionally cloned using 5' linkers 5'-AATTCGGCACGAG-3' and 5'-CTCGTGCCG-3'. Size selection of >400bp material gives average insert size ranging from 1-2 kb. Library was mass excised (from lambda-UniZAP-XR) and resulting single-stranded phagemids were prepped and tranformed into DH10B. Library contains 96.5% recombinants. References: J. Androl. 20:635-639 and Gene 25:263-269. Library constructed and donated by J. McCarrey, Ph.D. (Southwest Foundation for Biomedical Research, Dept. of Genetics); excision done by E.M. Eddy, Ph.D. (National Institutes of Health, National Institute of Environmental Health Sciences). Original lambda-based library is available through ATCC, catalog #63416.

text: 8 day

text: DH10B (phage-resistant)

text: testis

text: Mus musculus

text: XhoI

text: EcoRI

text: male

text: type A spermatogonia, pooled from multiple mice

text: McCarrey Eddy type A spermatogonia

text: phagemid (ampicillin resistant)

text: pBluescript SK+ (Stratagene)


characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: cDNA oligo dT-primed [5'-(GA)10-ACTAGTCTCGAGTTTTTTTTTTTTT-3'] and directionally cloned using 5' linkers 5'-AATTCGGCACGAG-3' and 5'-CTCGTGCCG-3'. Size selection of >400bp material gives average insert size ranging from 1-2 kb. Library was mass excised (from lambda-UniZAP-XR) and resulting single-stranded phagemids were prepped and tranformed into DH10B. Library contains 96% recombinants. References: J. Androl. 20:635-639 and Gene 25:263-269. Library constructed and donated by J. McCarrey, Ph.D. (Southwest Foundation for Biomedical Research, Dept. of Genetics); excision done by E.M. Eddy, Ph.D. (National Institutes of Health, National Institute of Environmental Health Sciences). Original lambda-based library is available through ATCC, catalog #63417.

text: 8 day

text: DH10B (phage-resistant)

text: testis

text: Mus musculus

text: XhoI

text: EcoRI

text: male

text: type B spermatogonia, pooled from multiple mice

text: McCarrey Eddy type B spermatogonia

text: phagemid (ampicillin resistant)

text: pBluescript SK+ (Stratagene)


text: Chinese National Human Genome Center at Shanghai

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Adult

text: SOLR

text: Homo sapiens

text: EcoRI

text: XhoI

text: Adrenal gland

text: ADA

text: pBluescript sk(-)


text: live

text: Generic

text: Adult

characteristic: dev-stage

text: SOLR

text: Homo sapiens

text: EcoRI

text: XhoI

text: Adrenal gland

text: ADB

text: pBluescript sk(-)


text: Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences

text: live

text: Generic

text: Adult

text: SOLR

text: Homo sapiens

characteristic: organism

text: EcoRI

text: XhoI

text: Adrenal gland

text: ADC

text: pBluescript sk(-)


text: live

text: Generic

text: Adult

text: Homo sapiens

text: adrenal cortico adenoma for Cushing's syndrome

text: Cu

text: pBluescript sk(-)


text: live

text: Generic

text: dendritic cells

characteristic: cell type

text: mature

text: Homo sapiens

text: sfiIA

text: sfiIB

text: DCA


text: Kazusa DNA Research Institute

text: live

text: Generic

text: The cDNA library was constructed from cells cultured in a carbon stress acclimatized condition in which carbon dioxide concentration in the bubbling gas was changed from 5% to 0.04%

text: Chlamydomonas reinhardtii

text: EcoRI

text: XhoI

characteristic: strain

text: Chlamydomonas reinhardtii 5% to 0.04% CO2

characteristic: title

text: pBluescriptII SK-


text: Kazusa DNA Research Institute

text: live

text: Generic

text: The cDNA library was constructed from cells cultured in a medium with bubbling air containing 5% carbon dioxide

characteristic: description

text: Chlamydomonas reinhardtii

text: EcoRI

text: XhoI

text: Chlamydomonas reinhardtii 5% CO2

text: pBluescriptII SK-


text: Columbia University, P & S

text: live

text: Generic

text: HO-1 melanoma cells

text: Homo sapiens

text: Human HO-1 melanoma cells


text: Kunming Medical College

text: live

text: Generic

text: Mus musculus

text: bone marrow

text: CX


text: Medical University of South Carolina

text: live

text: Generic

text: Penaeus vannamei

text: Sfi I

text: Sfi I

text: Hepatopancreas

text: Plasmid


text: Medical University of South Carolina

text: live

text: Generic

text: Penaeus setiferus

text: Sfi I

text: Sfi I

text: Hepatopancreas

text: Plasmid


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 2.2 kb. Constructed by Life Technologies.

text: DH10B (phage-resistant)

characteristic: lab-host

text: Xenopus laevis

text: NotI

text: SalI

text: oocytes

text: phagemid (ampicillin resistant)


text: Brigham and Women's Hospital, Harvard Medical School

text: live

text: Generic

text: mast cell (in vitro differentiated)

text: Mouse bone marrow-derived mast cells were developed by culturing progentiors for 3-6 weeks in 50% RPMI-1640 medium supplemented with 100 ng/ml c-kit ligand and 50% WEHI-3 cell-conditioned medium (as a source of interleukin 3). The first-strand cDNA was synthesized from mRNA using NotT priming (Invitrogen). The resulting cDNAs were ligated with an EcoRI/BstXI adaptor and then cloned into pcDNA3 mammalian expression vector by Invitrogen.

text: Mus musculus

text: EcoR I

text: Bxt XI

text: BALB/cJ

text: starting cells - non-fractionated bone marrow cells

text: Mouse Bone Marrow-derived Mast Cell Expression Library

text: plasmid

text: pcDNA3 (Invitrogen)


text: Rockefeller University Center for Biotechnology

text: live

text: Generic

text: obtained from differential display analysis with increased expression in psoriatic skin

text: adult

text: Homo sapiens

text: skin

text: Homo sapiens skin adult differential display


text: The Kansai Electric Power Co.

text: live

text: Generic

text: Avicennia marina

text: leaf

text: Avicennia marina leaf

text: lambda ZAPII


text: live

text: Generic

text: dendritic cells

text: mature

text: Homo sapiens

text: sfiIA

text: sfiIB

text: DCB


text: The Scripps Research Institute

text: live

text: Generic

text: The SEpT7T3D library is a subtracted cDNA library derived from a striatum cDNA library minus a cerebellum cDNA library. The library was prepared by the method 'Directional Tag PCR Subtraction'. This procedure has been described previously (Usui et al., J. Neurosci. 14:4915-4926, 1994; Thomas et al., J. Neurosci. Res. 52: 118-124, 1998)

text: Adult

text: Brain

characteristic: organ

text: Rattus norvegicus

text: Eco RI

text: Not I

text: Male

characteristic: sex

text: Sprague-Dawley

text: Plasmid

text: pT7T3D (Pharmacia)


characteristic: External Id

text: University of Edinburgh

text: live

text: Generic

text: Library was made from dissected testis germinal zone from adult male Ascaris suum collected from abbatoirs. Constructed by Michelle Lizotte-Waniewski for Alan Scott, Johns Hopkins University Medical School, Baltimore, MD.

text: Adult

text: Ascaris suum

text: EcoRI

text: XhoI

text: Male

text: Ascaris suum adult male testis germinal zone from Alan Scott

text: Lambda Zap II

characteristic: vector


text: Chinese National Human Genome Center at Shanghai

characteristic: INSDC center name

text: live

text: Generic

text: Adult

text: SOLR

text: Homo sapiens

text: EcoRI

text: XhoI

text: hepatocellular carcinoma

characteristic: tissue-type

text: GK

text: pBluescript sk(-)


characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Fetal

text: Homo sapiens

text: sfiIA

text: sfiIBl

text: hypothalamus

text: FHTA


text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Fetal

text: Homo sapiens

text: sfiIA

text: sfiIBl

text: hypothalamus

text: FHTB


text: live

text: Generic

characteristic: NCBI submission package

text: Adult

characteristic: dev-stage

text: SOLR

text: Homo sapiens

text: EcoRI

text: XhoI

text: Hypothalamus

text: HTB

text: pBluescript sk(-)


text: live

text: Generic

text: Adult

text: SOLR

text: Homo sapiens

characteristic: organism

text: EcoRI

text: XhoI

text: Hypothalamus

text: HTC

text: pBluescript sk(-)


text: live

text: Generic

text: Adult

text: SOLR

text: Homo sapiens

text: EcoRI

text: XhoI

text: Hypothalamus

text: HTE

text: pBluescript sk(-)


text: live

text: Generic

text: Adult

text: SOLR

text: Homo sapiens

text: EcoRI

text: XhoI

text: Hypothalamus

text: HTF

text: pBluescript sk(-)


text: live

text: Generic

text: Adult

text: Homo sapiens

text: sfiIA

text: sfiIB

text: pheochromocytoma

text: cdA


text: Medical College of Shantou University

text: live

text: Generic

characteristic: cell-line

text: The library is prepared by using PCR_SelectTM cDNA Subtraction Kit (Clontech). Briefly, the first cDNA is synthesized from polyA+RNA from the two types of cells being compared, SHEE(Immortalized esophageal epithelial cell line) and SHEEC(Esophageal cancer cell line), the second two subtractive hybridizations are performed, the third the entire population of molecules is subjected to PCR to amplify the desired differentially expressed sequences, then the cDNAs are directly inserted into a T/A cloning vector(pT_Adv vector, clontech), the last differentially expressed RNAs are identified by sequence and Northern hybridization analysis.

text: TOP10F' (Clontech)

text: Homo sapiens

text: EcoR I

text: Human esophageal cancer differentially expressed library

text: Plasmid


text: live

text: Generic

text: Anchor enzyme: NlaIII~ Tumor type: GBM (WHO grade IV). Patient age 4 months.~ Tissue supplier: ??~ Library constructed in the laboratory of G. Riggins, M.D., Ph.D. (Duke University).

text: brain

text: Homo sapiens

text: male

text: parietal lobe, primary tumor

characteristic: title

text: plasmid


text: Clemson University

text: live

text: Generic

text: CI16155 (Mla13)

characteristic: cultivar

text: C.I. 16155 (Mla13) plants were greenhouse grown in the R Wise lab at Iowa State University, Ames, IA; 7 day old green seedlings were challenged with isolate A27 (AvrMla13) of Blumeria graminis f. sp. hordei, and leaves were harvested 20 and 24 hr post-inoculation and snap frozen; uninoculated leaves were harvested 20 hr post-inoculation (Wei, Wise). In the TJ Close lab at the University of California, Riverside, total RNA was prepared from each sample pool, equal quantities of all three RNA pools were combined, poly(A) RNA was purified from the mixture, one cDNA library was made, and 1 million pfu were in vivo excised to give pBluescript SK(-) cDNA phagemids (Choi, Close). Phagemids were plated and picked at the Clemson University Genomics Institute (CUGI) (Begum, Palmer, Frisch, Atkins and Wing). Plasmid DNA preparations, DNA sequencing and sequence analysis were performed at CUGI (Wing, Yu, Frisch, Henry, Simmons, Oates, Rambo, Main). The sequence has been trimmed to remove vector sequence and contains a minimum of 100 bases of phred value 20 or above. For more details on library preparation and sequence analysis see http://www.genome.clemson.edu/projects/barley. To order this clone see http://www.genome.clemson.edu/orders Also see Close TJ, Wing R, Kleinhofs A, Wise R (2001) Genetically and physically anchored EST resources for barley genomics. Barley Genetics Newsletter 31:29-30. (http://wheat.pw.usda.gov/ggpages/bgn/31/cover.html)

characteristic: description

text: Hordeum vulgare subsp. vulgare

text: seedling green leaf

text: Hordeum vulgare seedling green leaf EST library HVcDNA0004 (Blumeria challenged)

text: Phage

text: lambdaZAP


text: Washington University School of Medicine

text: live

text: Generic

text: Harosoy

text: The Harosoy NIL was constructed and seed was provided by Dr. J. Specht, University of Nebraska (Shoemaker and Specht, 1995). The cDNA library was constructed from mRNA isolated from whole seedlings of 3 week old greenhouse grown plants. Complementary DNA was synthesized from mRNA using a primer consisting of a poly(dT) sequence with a XhoI restriction site and a 3' anchor. EcoRI adapters were ligated to the blunt-ended cDNA fragments followed by XhoI digestion. The cDNA fragments were directionally cloned into the EcoRI-XhoI restriction site of the pBluescript vector. The ligated cDNA fragments were transformed into DH10B host cells (GibcoBRL). This library was constructed in cooperation with Dr. Paul Keim's laboratory at Northern Arizona University.

characteristic: lab-host

text: Glycine max

text: EcoRI

text: XhoI

text: Whole seedling, 3 week old, greenhouse grown

text: plasmid

text: pBluescript II SK+


text: live

text: Generic

text: Harosoy

text: The Harosoy NIL was constructed and seed was provided by Dr. J. Specht, University of Nebraska (Shoemaker and Specht, 1995). The cDNA library was constructed from mRNA isolated from leaf tissue at various developmental stages of 3 week old greenhouse grown plants. Complementary DNA was synthesized from mRNA using a primer consisting of a poly(dT) sequence with a XhoI restriction site and a 3' anchor. EcoRI adapters were ligated to the blunt-ended cDNA fragments followed by XhoI digestion. The cDNA fragments were directionally cloned into the EcoRI-XhoI restriction site of the pBluescript vector. The ligated cDNA fragments were transformed into DH10B host cells (GibcoBRL). This library was constructed in cooperation with Dr. Paul Keim's laboratory at Northern Arizona University.

text: Glycine max

text: EcoRI

text: XhoI

text: Leaf, 3 week old, greenhouse grown

text: plasmid

text: pBluescript II SK+


text: live

text: Generic

text: Williams

text: The cDNA library was constructed from mRNA isolated germinating shoots of the cultivar Williams. The seeds were allowed to germinate for 24 hours prior to being cold stressed for 2 days at 4C. Complementary DNA was synthesized from mRNA using a primer consisting of a poly(dT) sequence with a XhoI restriction site. EcoRI adapters were ligated to the blunt-ended cDNA fragments followed by XhoI digestion. The cDNA fragments were directionally cloned into the EcoRI-XhoI restriction site of the pBluescript vector. The ligated cDNA fragments were transformed into DH10B host cells (GibcoBRL). This library was constructed in the laboratory of Dr. Randy Shoemaker.

text: Glycine max

text: EcoRI

text: XhoI

text: germinating shoots

text: plasmid

text: pBluescript II SK+


text: live

text: Generic

text: Williams 82

text: The cDNA library was constructed from mRNA isolated from germinating shoots of 3 day old seedling for the cultivar Williams 82. The seedlings were germinated in a growth chamber using germination paper in a solution containing 100ppm auxin. Complementary DNA was synthesized from mRNA using a primer consisting of a poly(dT) sequence with a XhoI restriction site. EcoRI adapters were ligated to the blunt-ended cDNA fragments followed by XhoI digestion. The cDNA fragments were directionally cloned into the EcoRI-XhoI restriction site of the pBluescript vector. The ligated cDNA fragments were transformed into DH10B host cells (GibcoBRL). This library was constructed in the laboratory of Dr. Randy Shoemaker.

text: Glycine max

text: EcoRI

text: XhoI

text: Germinating shoot, 3 day old seedling, auxin treatment

text: plasmid

text: pBluescript II SK+


text: Seoul National University

text: live

text: Generic

text: The cDNA library was constructed from rice leaves heavily infected by M. grisea. About 10% of cDNA clones in the library was fungal origin (M. grisea) and the 90% came from rice; The library was constructed from leaves 80 h after inoculated with Magnaporthe grisea strain 70-15

text: Oryza sativa

text: EcoRI

text: XhoI

text: leaves

text: Magnaporthe grisea infected rice cDNA library

text: lambda ZAP XAR


text: Shanghai Institute of Hematology, Rui-Jin Hospital

text: live

text: Generic

text: CD34+ hematopoietic stem/progenitor cell

characteristic: cell type

text: The insert is cloned randomly with the EcoRI digestion

text: Homo sapiens

text: EcoRI

text: cord blood

text: CB

text: pBluescript


characteristic: External Id

text: Shanghai Institute of Endocrinology, Rui-Jin Hospital

text: live

text: Generic

text: Adult

text: SOLR

text: Homo sapiens

text: EcoRI

text: XhoI

text: pituitary

text: pBluescript sk(-)


text: live

text: Generic

text: Adult

text: SOLR

text: Homo sapiens

text: EcoRI

text: XhoI

text: pituitary

text: NPA

text: pBluescript sk(-)

characteristic: vector


characteristic: INSDC center name

text: live

text: Generic

text: Adult

text: SOLR

text: Homo sapiens

text: EcoRI

text: XhoI

text: pituitary

text: NPB

text: pBluescript sk(-)


text: live

text: Generic

text: Adult

text: SOLR

text: Homo sapiens

text: EcoRI

text: XhoI

text: pituitary

characteristic: tissue-type

text: NPC

text: pBluescript sk(-)


characteristic: INSDC first public

text: live

text: Generic

text: Adult

text: SOLR

text: Homo sapiens

text: EcoRI

text: XhoI

text: pituitary

text: NPD

text: pBluescript sk(-)


characteristic: INSDC last update

text: live

text: Generic

text: Adult

text: Homo sapiens

text: sfiIA

text: sfiIB

text: pituitary tumor

text: TP


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

characteristic: INSDC status

text: Generic

text: Double-stranded cDNA was prepared from cell line RNA. 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.5 kb (range 0.9-4.0 kb). 14/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA). Note: this is a NIH_MGC Library.

text: DH10B (T1 phage-resistant)

text: prostate

characteristic: organ

text: Homo sapiens

text: SfiI (ggccgcctcggcc)

text: SfiI (ggccattatggcc)

text: adenocarcinoma

text: plasmid (chloramphenicol resistant, grown at 30C)

text: pDNR-LIB (Clontech)


text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Double-stranded cDNA was prepared from cell line RNA. 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.75 kb (range 0.9-4.0 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA). Note: this is a NIH_MGC Library.

text: DH10B (T1 phage-resistant)

text: testis

text: Homo sapiens

text: SfiI (ggccgcctcggcc)

text: SfiI (ggccattatggcc)

text: embryonal carcinoma

text: pDNR-LIB (Clontech)


text: live

text: Generic

text: 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.65 kb (range 0.5-4.0 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA). Note: this is a NIH_MGC Library.

text: DH10B (T1 phage-resistant)

text: kidney

text: Homo sapiens

characteristic: organism

text: SfiI (ggccgcctcggcc)

text: SfiI (ggccattatggcc)

text: pDNR-LIB (Clontech)


text: live

text: Generic

text: 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.9 kb (range 0.5-4.0 kb). 12/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA). Note: this is a NIH_MGC Library.

text: DH10B (T1 phage-resistant)

text: lung

text: Homo sapiens

text: SfiI (ggccgcctcggcc)

text: SfiI (ggccattatggcc)

text: pDNR-LIB (Clontech)


text: live

text: Generic

text: 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.3 kb (range 0.5-4.0 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA). Note: this is a NIH_MGC Library.

text: DH10B (T1 phage-resistant)

text: placenta

text: Homo sapiens

text: SfiI (ggccgcctcggcc)

text: SfiI (ggccattatggcc)

text: pDNR-LIB (Clontech)


text: Beijing Agro-Biotechnology Research Center

text: live

text: Generic

text: mature

characteristic: dev-stage

text: Vicia faba

text: leaf

text: Vicia faba mature leaf cDNA


text: Ludwig Institute for Cancer Research

text: live

text: Generic

text: A mini-library was made by cloning products derived from ORESTES PCR (U.S. Letters Patent application No. 196,716 - Ludwig Institute for Cancer Research) profiles into the pUC 18 vector. Reverse transcription of tissue mRNA and cDNA amplification were performed under low stringency conditions.

text: Adult

text: Homo sapiens

text: SmaI

text: SmaI

characteristic: title

text: Plasmid


text: Ludwig Institute for Cancer Research

text: live

text: Generic

text: Adult

text: breast

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: Ludwig Institute for Cancer Research

text: live

text: Generic

text: Adult

text: colon

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: Clemson University

text: live

text: Generic

characteristic: cultivar

text: Roots were harvested from plants grown under the following deficiencies/stresses: 10 mM Al, Zn, P, K, Fe,N. mRNA was isolated from indivdual treatments. Proportional aliquots of mRNA of each treatment were mixed and used for library construction.

text: 5-6 weeks old

text: SOLR

text: Solanum lycopersicum

text: 5' EcoRI

text: 3' XhoI

text: roots

text: tomato nutrient deficient roots

text: phagemid

text: pBluescriptSKmCUadapt


text: University of Wuerzburg (Biocenter)

text: live

text: Generic

text: epidermal cells

text: differentially expressed genes short after pupation of normal developing pupa and JH-treated pupa, respectively. DD-RT-PCR with anchored primers, visualisation by means of PAGE and silverstaining, topoTA-cloning of fragments.

characteristic: description

text: pupa

text: E. coli TOP10 (invitrogen)

text: Galleria mellonella

text: female and male

characteristic: sex

text: wild type

characteristic: strain

text: Epidermis

text: Galleria DD metamorphosis

text: Plasmid

text: pCRII-TOPO


characteristic: External Id

text: Chinese National Human Genome Center at Shanghai

text: live

text: Generic

characteristic: cell type

characteristic: lab-host

text: Homo sapiens

text: sfiIA

text: sfiIB

text: Bone marrow

text: BM


text: Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences

characteristic: INSDC center name

text: live

text: Generic

text: Homo sapiens

text: sfiIA

text: sfiIB

text: Bone marrow

characteristic: tissue-type

text: MDS

characteristic: vector


text: National Institute of Genetics

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: varied

text: Caenorhabditis elegans

text: Hermaphrodite

text: whole animal

text: unpublished oligo-capped cDNA library


text: Kanazawa University

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Bombyx mori

text: Bombyx mori library (Tsuzuki S)


text: Tokyo University of Marine Science and Technology, Graduate School of Marine Science and Technology

text: live

text: Generic

text: spleen

characteristic: organ

text: Anguilla japonica

characteristic: organism

text: Anguilla japonica spleen cDNA


text: Helix Research Institute

text: live

text: Generic

text: embryo, 10 weeks

characteristic: dev-stage

text: Homo sapiens

text: whole embryo, mainly head

characteristic: title


text: Helix Research Institute

text: live

text: Generic

text: embryo, 10 weeks

text: Homo sapiens

text: whole embryo, mainly body


text: Helix Research Institute

text: live

text: Generic

text: Homo sapiens

text: mammary gland


text: Clemson University

text: live

text: Generic

characteristic: cultivar

text: supplier: Boyce Thompson Institute; sequencing: The Institute for Genomic Research. Fruit were harvested at the breaker stage (first sign of lycopene accumulation on the blossom end of fruit). Fruit were cut in half and the seeds and locules were discarded prior to freezing the pericarp.

characteristic: description

text: breaker

text: SOLR

text: Solanum lycopersicum

text: XhoI

text: Pericarp

text: tomato breaker fruit

text: phagemid

text: pBluescriptSKmCUadapt


characteristic: External Id

text: Clemson University

text: live

text: Generic

text: Tissue supplied by Dave Garvin (USDA-ARS, Ithaca, NY 14850).

text: pre-anthesis stage

text: Solanum lycopersicum

text: root

text: tomato root, plant at pre-anthesis

text: phagemid

text: pBlueScript SK(-)


text: live

text: Generic

text: cDNA made by oligo-dT priming. Directionally cloned into EcoRI/XhoI sites using the following 5' adaptor: GGCACGAG(G). Size-selected >500bp for average insert size 1.8kb. Library constructed by Ling Hong in the laboratory of Gerald M. Rubin (University of California, Berkeley) using ZAP-cDNA synthesis kit (Stratagene) and Superscript II RT (Life Technologies). Note: this is a NIH_MGC Library.

text: DH10B (phage-resistant)

text: uterus

text: Homo sapiens

text: XhoI

text: EcoRI

text: leiomyosarcoma cell line


text: live

text: Generic

text: DH10B (phage-resistant)

text: B-cells

text: Homo sapiens

text: XhoI

text: EcoRI

text: primary B-cells from tonsils (cell line)


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: Double-stranded cDNA was prepared from cell line RNA. 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.65 kb (range 0.9-4.0 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA). Note: this is a NIH_MGC Library.

text: DH10B (T1 phage-resistant)

text: lung

text: Homo sapiens

text: SfiI (ggccgcctcggcc)

text: SfiI (ggccattatggcc)

text: mucoepidermoid carcinoma

text: pDNR-LIB (Clontech)


text: live

text: Generic

text: 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.85 kb (range 1.0-4.0 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA). Note: this is a NIH_MGC Library.

text: DH10B (T1 phage-resistant)

text: liver

text: Homo sapiens

text: SfiI (ggccgcctcggcc)

text: SfiI (ggccattatggcc)

text: plasmid (chloramphenicol resistant, grown at 30C)

text: pDNR-LIB (Clontech)


text: Helix Research Institute

text: live

text: Generic

text: teratocarcinoma

characteristic: cell type

characteristic: cell-line

text: mRNA from uninduced NT2 neuronal precursor cells

text: Homo sapiens


text: Helix Research Institute

characteristic: INSDC center name

text: live

text: Generic

text: teratocarcinoma

text: mRNA from NT2 neuronal precursor cells after 48-hours retinoic acid (RA) induction

text: Homo sapiens


text: Helix Research Institute

text: live

text: Generic

text: teratocarcinoma

text: mRNA from NT2 neuronal precursor cells after 2-weeks retinoic acid (RA) induction

text: Homo sapiens


text: Helix Research Institute

text: live

text: Generic

text: Homo sapiens

text: ovary, tumor tissue

characteristic: tissue-type


text: Helix Research Institute

text: live

text: Generic

text: Homo sapiens

text: placenta

characteristic: vector


text: Helix Research Institute

characteristic: INSDC first public

text: live

text: Generic

text: neuroepithelioma

text: SK-N-MC

text: Homo sapiens


text: Helix Research Institute

characteristic: INSDC last update

text: live

text: Generic

text: Homo sapiens

text: thyroid gland


text: Helix Research Institute

text: live

characteristic: INSDC status

text: Generic

text: umbilical vein endothelial cell (HUVEC)

text: primary endothelial cells

text: Homo sapiens


text: Helix Research Institute

text: live

text: Generic

characteristic: NCBI submission model

text: retinoblastoma

text: Homo sapiens


text: Universidade Federal de Minas Gerais

text: live

text: Generic

characteristic: NCBI submission package

text: amastigote

text: Trypanosoma cruzi

text: Tulahuen

characteristic: strain

text: Trypanosoma cruzi amastigote cDNA library


text: Martin Luther University Halle-Wittenberg, Medical Faculty

text: live

text: Generic

text: six days old preimplantation embryo

text: Oryctolagus cuniculus

characteristic: organism

text: hybrid strain Zika

text: rabbit blastocyst mRNA to cDNA


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.3 kb. Library constructed by Life Technologies.

text: 5 months

characteristic: dev-stage

text: DH10B (T1 phage-resistant)

characteristic: lab-host

text: testis

characteristic: organ

text: Mus musculus

text: NotI

text: SalI

text: male

characteristic: sex

text: phagemid (ampicillin resistant)


characteristic: External Id

text: VA Medical Center

text: live

text: Generic

text: Rattus norvegicus

text: Rat neurosphere PCR Select Library

characteristic: title


text: Aalborg Universitet

text: live

text: Generic

text: Field grown Kuras

characteristic: cultivar

text: Solanum tuberosum

text: Tuber

text: Mature tuber lambda ZAP

text: Lambda ZAP


characteristic: INSDC center name

text: live

text: Generic

text: cDNA made by oligo-dT priming. Directionally cloned into EcoRI/XhoI sites using the following 5' adaptor: GGCACGAG(G). Library constructed by Ling Hong in the laboratory of Gerald M. Rubin (University of California, Berkeley) using ZAP-cDNA synthesis kit (Stratagene) and Superscript II RT (Life Technologies). Note: this is a NIH_MGC Library.

characteristic: description

text: DH10B (phage-resistant)

text: lung

text: Homo sapiens

text: XhoI

text: EcoRI

text: large cell carcinoma


text: Ludwig Institute for Cancer Research

text: live

text: Generic

text: A mini-library was made by cloning products derived from ORESTES PCR (U.S. Letters Patent application No. 196,716 - Ludwig Institute for Cancer Research) profiles into the pUC 18 vector. Reverse transcription of tissue mRNA and cDNA amplification were performed under low stringency conditions.

text: Adult

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid

characteristic: vector


text: Ludwig Institute for Cancer Research

characteristic: INSDC first public

text: live

text: Generic

text: Adult

text: breast

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: Beijing Medical University

characteristic: INSDC last update

text: live

text: Generic

characteristic: cell-line

text: Homo sapiens

text: lung giant cell cancer

characteristic: tissue-type

text: Homo sapiens BE1


text: University Health Network

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: myeloma

characteristic: cell type

text: mRNA was purified from plasma cell leukemia patient's peripheral blood containing >95% myeloma. An oligo d(T)18 primer containing XhoI restriction site was used to prime first strand synthesis using M-MLV reverse transcriptase. To protect the cDNAs from XhoI digestion in subsequent cloning step, the nucleotide analogue 5-methyl-dCTP was added to the nucleotide mixture and [a-32P]dATP was added to monitor the quantity and quality of first strand synthesis. After second-strand synthesis and blunting of cDNA termini, EcoRI adapters were ligated, followed by kinase treatment and digestion with XhoI. The cDNAs were then size-fractionated using Sephacryl S-500 column and then ligated into EcoRI and XhoI digested Lambda Zap Express vector. The ligation product was packaged using Gigapack II packaging extract. The library had primary titre of approx. 1x106. Clones from the primary library were randomly selected for single pass sequencing.

text: Plasma cell leukemia

text: Homo sapiens

text: EcoRI

text: XhoI

text: male

text: Blood

text: Myeloma (PCL) cDNA library

text: Phage

text: Lambda Zap Express


text: University Health Network

text: live

text: Generic

text: myeloma

text: Myeloma cells from multiple myeloma patients' bone marrow were purified by magnetic cell sorting. mRNA were purified and an oligo d(T)18 primer containing XhoI restriction site was used to prime first strand synthesis using M-MLV reverse transcriptase. To protect the cDNAs from XhoI digestion in subsequent cloning step, the nucleotide analogue 5-methyl-dCTP was added to the nucleotide mixture and [a-32P]dATP was added to monitor the quantity and quality of first strand synthesis. After second-strand synthesis and blunting of cDNA termini, EcoRI adapters were ligated, followed by kinase treatment and digestion with XhoI. The cDNAs were then size-fractionated using Sephacryl S-500 column and then ligated into EcoRI and XhoI digested Lambda Zap Express vector. The ligation product was packaged using Gigapack II packaging extract. The library had primary titre of approx. 1x106. Clones from the primary library were randomly selected for single pass sequencing.

text: multiple myeloma

characteristic: dev-stage

text: Homo sapiens

characteristic: organism

text: EcoRI

text: XhoI

text: male

text: Blood

text: Myeloma (MYE) cDNA library

text: Phage

text: Lambda Zap Express


text: Clemson University

text: live

text: Generic

text: Tissue supplied by Dave Garvin (USDA-ARS, Ithaca, NY 14850).

text: etiolated radicle, 5 days post-imbibition

text: Solanum lycopersicum

text: root

text: tomato root, etiolated radicle

text: phagemid

text: pBlueScript SK(-)


text: University of Missouri

text: live

text: Generic

text: Abundant cDNAs were removed from the initial unnormalized cDNA library by subtraction with use of abundant cDNA pool. 'Rare' cDNAs are in pBluescript SK(-)

text: adults

text: Caenorhabditis elegans

text: hermaphrodite

characteristic: sex

text: Bristol N2

characteristic: strain

text: 'Rare' cDNA library from Caenorhabditis elegans adults

text: pBluescript SK(-)


characteristic: External Id

text: live

text: Generic

text: mesenchymal stem cells

text: fetal

text: Homo sapiens

text: Human fetal mesenchymal stem cells library

characteristic: title


text: Pusan National University

characteristic: INSDC center name

text: live

text: Generic

text: Homo sapiens

text: Homo sapiens HepG2


text: University of Edinburgh

text: live

text: Generic

text: Constructed by Diane Redmond for David Knox, Moredun Institute, Edinburgh using primers directed to the nematode splice leader (SL1) and the oligo d(T) tail. Primary library titre was 0.9x10^5 pfu.

characteristic: description

text: Egg

text: Haemonchus contortus

text: EcoRI

text: XhoI

text: Mixed

text: Moredun

text: Haemonchus contortus egg SL1 from David Knox

text: Phage

text: Lambda Uni-Zap XR (Stratagene)


text: University of Edinburgh

text: live

text: Generic

text: Constructed by Diane Redmond for David Knox, Moredun Institute, Edinburgh using primers directed to the nematode splice leader (SL1) and the oligo d(T) tail. Primary library titre was 1x10^5 pfu.

text: Haemonchus contortus

text: EcoRI

text: XhoI

text: Mixed

text: Moredun

text: Haemonchus contortus L3 SL1 from David Knox

text: Phage

text: Lambda Uni-Zap XR (Stratagene)

characteristic: vector


text: University of Edinburgh

characteristic: INSDC first public

text: live

text: Generic

text: Haemonchus contortus

text: EcoRI

text: XhoI

text: Mixed

text: Moredun

text: Haemonchus contortus L4 SL1 from David Knox

text: Phage

text: Lambda Uni-Zap XR (Stratagene)


text: Humboldt University Hospital, Charite

characteristic: INSDC last update

text: live

text: Generic

text: The library was generated by suppression subtractive hybridization (SSH).

text: TOP10F' (ampicillin resistant)

characteristic: lab-host

text: lung

characteristic: organ

text: Homo sapiens

text: NotI

text: SalI

text: male

text: metastatic small cell lung cancer (SCLC)

text: Human H526 cDNA library

text: Plasmid

text: pCR2.1 (Invitrogen)


text: Humboldt University Hospital, Charite

text: live

characteristic: INSDC status

text: Generic

text: HBEC

characteristic: cell-line

text: TOP10F' (ampicillin resistant)

text: lung

text: Homo sapiens

text: NotI

text: SalI

text: Human bronchial epithelial cells

characteristic: tissue-type

text: Human HBEC cDNA library

text: Plasmid

text: pCR2.1 (Invitrogen)


text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Anchor enzyme: NlaIII~ Tumor type: primary tumor in posterior fossa. Adult mouse PTCH +/1 knockout mouse constructed in the laboratory of Matt Scott (Stanford University)~ Tissue supplier: ??~ Library constructed in the laboratory of G. Riggins, M.D., Ph.D. (Duke University).

text: brain

text: Mus musculus

text: medulloblastoma

text: plasmid


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.03kb. Library constructed by Life Technologies, cat # 11132-018.

text: DH10B (T1 phage-resistant)

text: skin, normal, 4 pooled samples

text: Homo sapiens

characteristic: organism

text: NotI

text: SalI

text: phagemid (ampicillin resistant)


text: University of Wisconsin

text: live

text: Generic

text: Mus musculus

text: Mus musculus C57BL/6J (Dioxin Gene Expression Project)


text: RIKEN Genomic Sciences Center

text: live

text: Generic

text: Columbia

characteristic: cultivar

text: Arabidopsis thaliana


text: Kanazawa University School of Medicine

text: live

text: Generic

text: mRNA differentially expressed between tumor and normal tissues of human esophagus

text: XL-1blue MRF (STRATAGENE)

text: Homo sapiens

text: male

characteristic: sex

text: esophageal squamous cell carcinoma

text: Human esophageal carcinoma mRNA

text: Plasmid

text: pGEM-T (Promega)


characteristic: External Id

text: University of Newcastle-Upon-Tyne, The Medical School

text: live

text: Generic

text: Danio rerio

text: 717 created by Bernhard Korn


text: live

text: Generic

text: Danio rerio

text: Danio rerio library (Lynch N)

characteristic: title


text: Dept. of Biochemistry, Medical College, Tongji University

characteristic: INSDC center name

text: live

text: Generic

text: 7 weeks postnatal

characteristic: dev-stage

text: Mus musculus

text: male

characteristic: strain

text: brain

text: Differentially expressed cDNA library of brain of MPTP-treated C57BL mice


text: Medical College of Ohio

characteristic: INSDC first public

text: live

text: Generic

text: Differential display of mRNA using a modification of the ClonTech kit

characteristic: description

text: 31-34 days old

text: kidney

text: Rattus norvegicus

text: male

text: SS/Jr (inbred Dahl salt-sensitive)

text: Rat kidney differential display bands, strain = SS/Jr


text: Medical College of Ohio

characteristic: INSDC last update

text: live

text: Generic

text: 31-34 days old

text: kidney

text: Rattus norvegicus

text: male

text: SR/Jr (inbred Dahl salt-resistant)

text: Rat kidney differential display bands, strain = SR/Jr


text: Medical College of Ohio

text: live

text: Generic

text: 31-34 days old

text: kidney

characteristic: organ

text: Rattus norvegicus

text: male

text: S.R-Cyp11b (congenic strain)

text: Rat kidney differential display bands, strain = S.R-Cyp11b


text: Medical College of Ohio

text: live

characteristic: INSDC status

text: Generic

text: 31-34 days old

text: kidney

text: Rattus norvegicus

text: male

text: S.WKY(2) (congenic strain)

text: Rat kidney differential display bands, strain = S.WKY(2)


text: Molecular Biology

text: live

text: Generic

text: Homo sapiens

text: gastric cancer cell line

text: human gastric cancer cell line

text: pUCm-T

characteristic: vector


text: Lawrence Livermore National Laboratory

text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.9 kb. Constructed by Life Technologies. Note: this is a NCI_CGAP Library.

text: DH10B (T1 phage-resistant)

characteristic: lab-host

text: liver

text: Mus musculus

text: NotI

text: SalI

text: FVB/N

text: phagemid (ampicillin resistant)


text: University of St. Andrews

text: live

text: Generic

characteristic: NCBI submission model

text: Cyprinus carpio

text: Cyprinus carpio library (Hall T)


text: University of St. Andrews

text: live

text: Generic

characteristic: NCBI submission package

text: Clupea harengus

text: Clupea harengus library (Hall T)


text: University of St. Andrews

text: live

text: Generic

text: Gadus morhua

characteristic: organism

text: Gadus morhua library (Hall T)


text: University of St. Andrews

text: live

text: Generic

text: Danio rerio

text: Danio rerio library (Hall T)


text: Ludwig Institute for Cancer Research

text: live

text: Generic

text: A mini-library was made by cloning products derived from ORESTES PCR (U.S. Letters Patent application No. 196,716 - Ludwig Institute for Cancer Research) profiles into the pUC 18 vector. Reverse transcription of tissue mRNA and cDNA amplification were performed under low stringency conditions.

text: Adult

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: Ludwig Institute for Cancer Research

text: live

text: Generic

text: Adult

text: colon

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: Washington University School of Medicine

text: live

text: Generic

text: This library was constructed by Steve Fogarty, Robert Cole, and Keliang Tang at Washington University. cDNAs were synthesized from poly(A)+ RNA by oligo d(T) priming, size-selected and directionally cloned into the Uni-ZAP XR lambda vector (Stratagene). The primary library was mass excised as phagemids and rescued in SOLR cells. The plasmid library was recovered from the SOLR cells and transformed in mass into DH10B (GeneHog, Research Genetics, Inc.) for sequencing. WARNING:the library may contain a small percentage of contaminents from human fibroblast cells.

text: Tachyzoite

text: Neospora caninum

text: EcoRI

text: XhoI

text: Nc 1314 Tachyzoite cDNA

text: pBluescript SK-


text: Instituto de Investigaciones Biotecnologicas Universidad Nacional de San Martin

text: live

text: Generic

text: cDNA was synthesized by reverse transcription of total RNA with oligo dT primer. cDNA was digested with RsaI and ligated with the adaptors 1 (5'-CTAATACGACTCACTATAGGGCTCGAGCGGCCGCCCGGGCAGGT-3') and 2 (5'-GTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGT-3'). Two cDNA populations were then hybridizated and after that two PCR reactions were performed. The first one with the PCR primer 1 (5'-CTAATACGACTCACTATAGGGC-3') and the second reaction with Nested PCR primer 1 (5'-TCGAGCGGCCGCCCGGGCAGGT-3') and Nested PCR primer 2 (5'-AGCGTGGTCGCGGCCGAGGT-3'). These PCR products were cloned in pGEM-Teasy vector (Promega).

text: adult

text: brain

text: Rattus norvegicus

text: RsaI

text: RsaI

text: male

text: Sprague Dawley

text: hippocampus

characteristic: tissue-type

text: PCR-SelectTM cDNA Subtraction Kit, Clontech. (cat# K1804-1)

text: Plasmid

text: pGEM-T easy, Promega.


text: Northwestern University

text: live

text: Generic

text: Outer hair cell

characteristic: cell type

text: Adult

text: Organ of Corti

text: Meriones unguiculatus

text: Cochlear outer hair cell minus inner hair cell


characteristic: External Id

text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: cDNAs were oligo-dT primed and directionally cloned. Staging according to Nieuwkoop and Faber. Library was constructed by N. Garrett, E. Bellefroid, and A.M. Zorn, (Wellcome/CRC Institute).

text: DH10B (phage-resistant)

text: Xenopus laevis

text: NotI

text: EcoRI

text: pooled embryos, stage 19-26

text: Wellcome CRC pRN3 St19 26

text: plasmid (ampicillin resistant)


text: live

text: Generic

text: cDNAs were oligo-dT primed and directionally cloned. Staging according to Nieuwkoop and Faber. Library was constructed by A.M. Zorn (Wellcome/CRC Institute).

text: DH10B (phage-resistant)

text: Xenopus laevis

text: NotI

text: EcoRI

text: dorsal lip

text: Wellcome CRC pRN3 dorsal lip

characteristic: title

text: plasmid (ampicillin resistant)


characteristic: INSDC center name

text: live

text: Generic

text: cDNAs were oligo-dT primed and directionally cloned. Staging according to Nieuwkoop and Faber. Library was constructed by N. Garrett, P. LeMaire, A.M. Zorn, and J.B. Gurdon (Wellcome/CRC Institute).

text: DH10B (phage-resistant)

text: Xenopus laevis

text: NotI

text: EcoRI

text: oocyte

text: Wellcome CRC pRN3 oocyte

text: plasmid (ampicillin resistant)


text: live

text: Generic

text: cDNAs were oligo-dT primed and directionally cloned. Staging according to Nieuwkoop and Faber. Library is subtracted and was constructed by N. Garrett, E Bellefroid, and A.M. Zorn, (Wellcome/CRC Institute).

text: DH10B (phage-resistant)

text: Xenopus laevis

text: NotI

text: EcoRI

text: egg, subtracted by stage 13-17 animal cap

text: Wellcome CRC pRN3 St13 17 egg animal cap

text: plasmid (ampicillin resistant)


text: The Institute for Genomic Research

text: live

text: Generic

text: spherule

characteristic: dev-stage

text: SOLR

text: Coccidioides posadasii

text: Coccidioides posadasii spherule cDNA library

text: phagemid

text: pBluescript SK(-)


text: The Sanger Centre

text: live

text: Generic

text: cDNA fragment isolated using a cDNA end rescue technique

text: adult

text: Homo sapiens

text: Brain

text: Homo sapiens adult brain (Stavrides GS)


text: The Sanger Centre

text: live

text: Generic

text: fetal

text: Homo sapiens

text: Brain

text: Homo sapiens fetal brain (Stavrides GS)


text: The Sanger Centre

text: live

text: Generic

text: Homo sapiens

text: Testis

text: Homo sapiens Testis (Stavrides GS)


text: The Sanger Centre

text: live

text: Generic

text: adult

text: Homo sapiens

text: Lung

text: Homo sapiens adult lung (Stavrides GS)


text: The Sanger Centre

text: live

text: Generic

text: fetal

text: Homo sapiens

text: Lung

text: Homo sapiens fetal lung (Stavrides GS)


text: The Sanger Centre

text: live

text: Generic

text: fetal

text: Homo sapiens

text: Liver

text: Homo sapiens fetal liver (Stavrides GS)


text: The Sanger Centre

text: live

text: Generic

text: adult

text: Homo sapiens

text: Heart

text: Homo sapiens adult heart (Stavrides GS)


text: Clemson University

text: live

text: Generic

text: Morex

characteristic: cultivar

text: Seeds were surface sterilized then germinated under axenic conditions in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Five-day old seedling roots were then harvested, total RNA was prepared, poly(A) RNA was purified, one primary unamplified cDNA library was made, and 1 million pfu were in vivo excised to give pBluescript SK(-) cDNA phagemids. These steps were performed in the TJ Close laboratory at the University of California, Riverside (Choi, Close, Fenton). Phagemids were plated and picked at the Clemson University Genomics Institute (CUGI) (Begum, Palmer, Frisch, Atkins and Wing). Plasmid DNA preparations, DNA sequencing and sequence analysis were performed at CUGI (Wing, Yu, Frisch, Henry, Simmons, Oates, Rambo, Main). The sequence has been trimmed to remove vector sequence and contains a minimum of 100 bases of phred value 20 or above. For more details on library preparation and sequence analysis see http://www.genome.clemson.edu/projects/barley. To order this clone see http://www.genome.clemson.edu/orders Also see Close TJ, Wing R, Kleinhofs A, Wise R (2001) Genetically and physically anchored EST resources for barley genomics. Barley Genetics Newsletter 31:29-30. (http://wheat.pw.usda.gov/ggpages/bgn/31/cover.html)

text: Hordeum vulgare subsp. vulgare

text: Seedling root

text: Hordeum vulgare seedling root EST library HVcDNA0007 (Etiolated and unstressed)

text: Phage

text: lambdaZAP


text: Dept of AFNS, University of Alberta

characteristic: INSDC first public

text: live

text: Generic

text: muscle

text: Young adult

text: Skeletal muscle

text: Bos taurus

text: EcoR I

text: Xho I

text: two males and one female mixed

characteristic: sex

text: masseter, longissmusclorsi, biceps femoris, semimembrances

text: Bovine Mixed Skeletal Muscle cDNA Library

text: Phage


text: The Institute for Genomic Research

characteristic: INSDC last update

text: live

text: Generic

text: Combination of ROV, RBR, RKI, RLI, RPL, RLU, REM, RMU, RSP, RHE, RPC, RPN

characteristic: description

text: Rattus norvegicus

text: mixed tissue

text: Rat Gene Index, normalized rat, Rattus norvegicus cDNA


text: Lawrence Livermore National Laboratory

text: live

characteristic: INSDC status

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.3 kb. Constructed by Life Technologies. Note: this is a NCI_CGAP Library.

text: DH10B (T1 phage-resistant)

text: salivary gland

characteristic: organ

text: Mus musculus

text: NotI

text: SalI

text: FVB/N

characteristic: strain

text: phagemid (ampicillin resistant)


text: University of California Los Angeles

text: live

text: Generic

characteristic: NCBI submission model

text: Human Umbilical Vein Endothelial Cell

characteristic: cell-line

text: Homo sapiens

text: EcoRI

text: HUVEC cDNA Library

text: Plasmid

text: Lambda UniZAP Vector

characteristic: vector


text: US Department of Agriculture, Agriculture Research Service, Pacific West Area, Western Regional Research Center

text: live

text: Generic

characteristic: NCBI submission package

text: F2 from 2-12-4-8-1-1-1-(1) x PI36909-12-811-(1)

text: Plants were grown in a growth chamber at the University of California, Davis (Akhunov). Premeiotic anthers were harvested, total RNA and poly(A) RNA were prepared, from each tissue and then pooled, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Akhunov, Chin, Choi, Close, Fenton, Kianian, Otto, Simons, Zhang) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

text: Premeiotic anthers

text: E. coli SOLR

characteristic: lab-host

text: Aegilops speltoides

characteristic: organism

text: EcoRI

text: XhoI

text: Anther

characteristic: tissue-type

text: Aegilops speltoides anther cDNA library

text: Phagemid

text: Lambda Uni-ZAP XR, excised phagemid


characteristic: External Id

characteristic: INSDC center name

text: live

text: Generic

text: Plants were grown in a growth chamber at North Dakota State University (Kianian, Otto, Simons). Tissues collected from seven-day etiolated seedling leaf, stem, root and seed; leaf from plant at fourth leaf stage; spike from pre-anthesis through 20 days after anthesis; flag leaf; leaf and stem tissue from tillers. and root. Total RNA and poly(A) RNA were prepared from each tissue and then pooled, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Akhunov, Chin, Choi, Close, Fenton, Kianian, Otto, Simons, Zhang) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

text: Different growth stages

text: E. coli SOLR

text: Triticum turgidum

text: EcoRI

text: XhoI

text: All tissues

text: Triticum turgidum L. var. durum (durum wheat) whole plant cDNA library

characteristic: title

text: Phagemid

text: Lambda Uni-ZAP XR, excised phagemid


text: Humboldt University Hospital, Charite

characteristic: INSDC first public

text: live

text: Generic

text: The library was generated by suppression subtractive hybridization (SSH).

text: TOP10F' (ampicillin resistant)

text: lung

text: Homo sapiens

text: NotI

text: SalI

text: non-small cell lung carcinoma (NSCLC)

text: Human H2170 cDNA library

text: Plasmid

text: pCR2.1 (Invitrogen)


text: Humboldt University Hospital, Charite

text: live

text: Generic

text: HBEC

text: TOP10F' (ampicillin resistant)

text: lung

text: Homo sapiens

text: NotI

text: SalI

text: human bronchial epithelial cells

text: Human HBECII cDNA library

text: Plasmid

text: pCR2.1 (Invitrogen)


text: University of Florida

text: live

text: Generic

text: Total RNA was extracted from asynchronous blood stage forms of the cloned ANKA isolate of P. berghei, grown in Wistar rats to 30% parasitemia and 2-5% gametocytemia. Contaminating host white cells had previously been removed and final host cell contamination estimated to be approximately 5%. PolyA+ RNA was extracted and reverse transcribed using an oligo dT-XhoI primer (Lambda ZAP II cDNA cloning kit, Stratagene). Second strand cDNA was made following the manufacturer's protocol. EcoRI adaptors were ligated to the cDNA, and fragments were ligated into EcoRI/XhoI digested vector.

text: asynchronous blood stage

characteristic: dev-stage

text: Wistar rats

text: Plasmodium berghei

text: EcoRI

text: XhoI

text: ANKA clone HP (gametocyte producer)

text: Pb cDNA #17, Tommaso Pace, Marta Ponzi, and Clara Frontali

text: phagemid

text: pBluescript II vector DNA, excised from Lamda ZAP II.


text: University of Florida

characteristic: INSDC last update

text: live

text: Generic

text: Total RNA was extracted from asynchronous blood stage forms of the cloned ANKA isolate of P. berghei grown in laboratory Swiss white mice. Contaminating host white cells had previously been removed using a novel biomagnetic bead protocol (J. Carlton et al., manuscript in preparation). PolyA+ RNA was extracted and reverse transcribed using an oligo dT-XhoI primer. Second strand cDNA was prepared using RNase H and DNA polymerase I. EcoR I adaptors were ligated to the cDNA, and it was digested with XhoI. Fragments were size selected, and those between 1-5 kb ligated into EcoRI /XhoI digested vector.

text: asynchronous blood stage

text: Swiss white mice

text: Plasmodium berghei

text: EcoRI

text: XhoI

text: ANKA clone 15cy1 (clone of the ANKA 8417 HP clone)

text: Pb cDNA #20, Charles Yowell and Jane Carlton

text: phagemid


text: Instituto de Biologia Molecular do Parana

text: live

text: Generic

text: Trypanosoma cruzi

text: Trypanosoma cruzi differential display cDNA library


text: Roslin Institute

text: live

text: Generic

text: Sus scrofa

text: adipose

text: Sus scrofa adipose


text: University of Maryland

text: live

text: Generic

text: cDNA sythesized from RNA isolated from developing terminals buds induced by short-day photoperiods.

characteristic: description

text: developing bud

text: terminal bud

text: Populus trichocarpa x Populus deltoides

text: EcoRI

text: EcoRI

text: vegetative

text: Poplar first strand cDNA

text: plasmid


text: live

characteristic: INSDC status

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.6 kb. Library constructed by Life Technologies.

text: 10 weeks

text: DH10B (T1 phage-resistant)

text: liver

text: Mus musculus

text: NotI

text: SalI

text: female

characteristic: sex

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.8 kb. Library constructed by Life Technologies.

text: 10 weeks

text: DH10B (T1 phage-resistant)

text: brain

characteristic: organ

text: Mus musculus

text: NotI

text: SalI

text: female

text: phagemid (ampicillin resistant)


text: Washington University School of Medicine

text: live

text: Generic

characteristic: NCBI submission model

text: Williams

characteristic: cultivar

text: The cDNA library was constructed from mRNA isolated from unexpanded leaves and the shoot tips of 2 week old seedling from the cultivar Williams. The 2 week old seedlings were salt stressed in a solution of 500mM NaCl for 3 days prior to harvesting. Complementary DNA was synthesized from mRNA using a primer consisting of a poly(dT) sequence with a XhoI restriction site. EcoRI adapters were ligated to the blunt-ended cDNA fragments followed by XhoI digestion. The cDNA fragments were directionally cloned into the EcoRI-XhoI restriction site of the pBluescript vector. The ligated cDNA fragments were transformed into DH10B host cells (GibcoBRL). This library was constructed in the laboratory of Dr. Randy Shoemaker.

text: Glycine max

text: EcoRI

text: XhoI

text: Leaf and shoot tip, salt stressed, 2 week old seedling

text: plasmid

text: pBluescript II SK+


text: live

text: Generic

text: Williams 82

text: The cDNA library was constructed from mRNA isolated from drought stressed leaf tissue of the cultivar Williams 82. The month old greenhouse grown plants were deprived of water for 3 days prior to harvesting the stressed leaf tissue. Complementary DNA was synthesized from mRNA using a primer consisting of a poly(dT) sequence with a XhoI restriction site. EcoRI adapters were ligated to the blunt-ended cDNA fragments followed by XhoI digestion. The cDNA fragments were directionally cloned into the EcoRI-XhoI restriction site of the pBluescript vector. The ligated cDNA fragments were transformed into DH10B host cells (GibcoBRL). This library was constructed in the laboratory of Dr. Randy Shoemaker.

text: Glycine max

text: EcoRI

text: XhoI

text: Leaf, drought stressed, 1 month old plants, greenhouse grown

text: plasmid

text: pBluescript II SK+

characteristic: vector


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

characteristic: NCBI submission package

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.57 kb. Constructed by Life Technologies. Note: this is a NCI_CGAP Library.

text: DH10B (T1 phage-resistant)

text: brain

text: Homo sapiens

text: NotI

text: SalI

text: glioblastoma with EGFR amplification

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 2.3 kb. Constructed by Life Technologies. Note: this is a NCI_CGAP Library.

text: DH10B (T1 phage-resistant)

characteristic: lab-host

text: brain

text: Homo sapiens

text: NotI

text: SalI

text: anaplastic oligodendroglioma with 1p/19q loss

text: phagemid (ampicillin resistant)


text: Ludwig Institute for Cancer Research

text: live

text: Generic

text: A mini-library was made by cloning products derived from ORESTES PCR (U.S. Letters Patent application No. 196,716 - Ludwig Institute for Cancer Research) profiles into the pUC 18 vector. Reverse transcription of tissue mRNA and cDNA amplification were performed under low stringency conditions.

text: Adult

text: Homo sapiens

characteristic: organism

text: SmaI

text: SmaI

text: Plasmid


text: Ludwig Institute for Cancer Research

text: live

text: Generic

text: Adult

text: breast

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: Ludwig Institute for Cancer Research

text: live

text: Generic

text: Adult

text: colon

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: Ludwig Institute for Cancer Research

text: live

text: Generic

text: Adult

text: marrow

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: The Chinese University of Hong Kong

text: live

text: Generic

text: Most cell types

characteristic: cell type

characteristic: cell-line

text: The cephalothorax content of 5 adult shrimp (sex not determined) was used in the RNA preparation for this library. This includes most organ and tissue types of shrimp: nervous system, digestive system, endocrine system, heart, gills, eyestalk, epidermis, gonads etc. mRNA isolated from cephalothorax tissue was used to construct a unidirectional library in UniZap. Clones excised in vivo to create phagemids in pBluescript SK- for sequencing.

text: adult

text: SOLR

text: Most organ systems

text: Penaeus monodon

text: EcoRI

text: XhoI

text: Most tissue types

characteristic: tissue-type

text: Shrimp Whole Cephalothorax UniZap library

text: Lambda Phage

text: UNiZap


characteristic: External Id

text: Second Military Medical University

characteristic: INSDC center name

text: live

text: Generic

text: 24 hours post-infection

text: Anopheles stephensi

text: female

text: Hor

characteristic: strain

text: whole body

text: Mosquito Genes Pool Related Malaria Infection

characteristic: title


text: Shanghai Tiedao University Medical School

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Differentially expressed cDNA library of optic tectum of pigeon with removal of left retina

characteristic: description

text: 10 day postnatal

characteristic: dev-stage

text: Columba livia

text: female and male

text: optic tecta


text: Ludwig Institute for Cancer Research

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Adult

text: stomach

characteristic: organ

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: INRA-ENSAR

text: live

text: Generic

characteristic: NCBI submission package

text: random amplified cDNA clones; direct sequencing of RT-PCR products

text: adult (9 weeks)

text: liver

text: Gallus gallus

text: male

characteristic: sex

text: experimental crosses


text: Chinese University of Hong Kong

text: live

text: Generic

text: Lentinula edodes

characteristic: organism

text: Lentinula edodes L54


text: Children's Memorial Research Center

text: live

text: Generic

text: The UI-R-CA1 library is a subtracted library derived from the following tissues: thalamus, cerebellum, hypothalamus, medulla, pons, midbrain, cerebral cortex, corpus striatum, testis, and hippocampus. For a detailed description of the library from which this clone was derived, please visit our web site at ratest.eng.uiowa.edu. The subtraction has been previously described in (Bonaldo, Lennon and Soares, Genome Research 6:791-806, 1996)

text: DH10B (Life Technologies)

characteristic: lab-host

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid

characteristic: vector


text: Children's Memorial Research Center

text: live

text: Generic

text: The UI-R-BS2 library is a subtracted library derived from 13 dpc whole embryo tissue. For a detailed description of the library from which this clone was derived, please visit our web site at ratest.eng.uiowa.edu. The subtraction has been previously described in (Bonaldo, Lennon and Soares, Genome Research 6:791-806, 1996)

text: embryonic 13 dpc

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid


text: Children's Memorial Research Center

text: live

text: Generic

text: The UI-R-CJ0 library is a subtracted library derived from the following tissues: mammary gland, pregnant uterus, testis. For a detailed description of the library from which this clone was derived, please visit our web site at ratest.eng.uiowa.edu. The subtraction has been previously described in (Bonaldo, Lennon and Soares, Genome Research 6:791-806, 1996)

text: ADULT

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid


text: Children's Memorial Research Center

text: live

text: Generic

text: The UI-R-BJ2 library is a subtracted library derived from the following tissues: heart, atrium at 15 dpc, ventricle at 16.5 dpc, atrium at 16.5 dpc, ventricle at 13 dpc, ventricle at 15 dpc, AV canal at 15 dpc. For a detailed description of the library from which this clone was derived, please visit our web site at ratest.eng.uiowa.edu. The subtraction has been previously described in (Bonaldo, Lennon and Soares, Genome Research 6:791-806, 1996)

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid


text: Children's Memorial Research Center

text: live

text: Generic

text: The UI-R-CM0 library is a non-normailized library constructed from rat hear tissue. For a detailed description of the library from which this clone was derived, please visit our web site at ratest.eng.uiowa.edu.

text: ADULT

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid


text: Children's Memorial Research Center

text: live

text: Generic

text: The UI-R-CN0 library is a normalized library constructed from the following tissues: brown adipose, penis, salivary glands, bladder, fundus region of the stomach, cervix, seminal vesicles. For a detailed description of the library from which this clone was derived, please visit our web site at ratest.eng.uiowa.edu. The subtraction has been previously described in (Bonaldo, Lennon and Soares, Genome Research 6:791-806, 1996)

text: ADULT

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid


text: Ji Lin University

text: live

text: Generic

text: neuroepithelium

characteristic: cell type

text: Homo sapiens

text: male

text: brain

characteristic: tissue-type

text: human fetal brain neuroepithelium cDNA library


characteristic: External Id

text: King's College London

characteristic: INSDC center name

text: live

text: Generic

text: The library was prepared using protocol given by supplier (Stratagene).

text: adult

text: Lumbricus rubellus

text: whole worm

text: Earthworm Lambda Zap Express Library

text: phagemid

text: pBK-CMV


text: Washington University School of Medicine

text: live

text: Generic

text: Williams

characteristic: cultivar

text: The cDNA library was constructed from mRNA isolated from degenerating cotyledons of 9-10 day old etiolated seedlings for the cultivar Williams. Complementary DNA was synthesized from mRNA using a primer consisting of a poly(dT) sequence with a XhoI restriction site. EcoRI adapters were ligated to the blunt-ended cDNA fragments followed by XhoI digestion. The cDNA fragments were directionally cloned into the EcoRI-XhoI restriction site of the pBluescript vector. The ligated cDNA fragments were transformed into DH10B host cells (GibcoBRL). This library was constructed in the laboratory of Dr. Randy Shoemaker.

text: Glycine max

text: EcoRI

text: XhoI

text: Degenerating cotyledons, 9-10 day old etiolated seedling

characteristic: title

text: plasmid

text: pBluescript II SK+


characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: cDNA made by oligo-dT priming. Directionally cloned using the following adaptors: 5'-TCGACCCACGCGTCCG-3' and 5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCT(15)-3'. Size selected > 1 kb for average insert length 1.87 kb. This is a primary library, non-amplified. Library constructed by Life Technologies and donated by J. Lupski, M.D./Ph.D. (Baylor College of Medicine) and is available through Life Technologies.

text: adult, 70 yr

text: Homo sapiens

text: NotI

text: SalI

text: male

text: sciatic nerve

text: plasmid (ampicillin resistant, 200 ug/ml)

text: pCMV-SPORT6 (Life Technologies)


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.5 kb. Constructed by Life Technologies.

characteristic: description

text: DH10B (T1-phage resistant)

text: breast

text: Homo sapiens

text: NotI

text: SalI

text: invasive ductal carcinoma, 3 pooled samples

text: phagemid (ampicillin resistant)


text: live

characteristic: INSDC status

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 2.6 kb. Constructed by Life Technologies. Note: This is a Xenopus Gene Collection (XGC) library.

text: adult

characteristic: dev-stage

text: DH10B (phage-resistant)

text: lung

text: Xenopus laevis

text: NotI

text: SalI

text: phagemid (ampicillin resistant)


text: National Cancer Institute / NIH

text: live

text: Generic

characteristic: NCBI submission model

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 2.0 kb. Constructed by Life Technologies.

text: DH10B (phage-resistant)

text: ovary

characteristic: organ

text: Xenopus laevis

text: NotI

text: SalI

text: female

text: phagemid (ampicillin resistant)


text: USDA, ARS, US Meat Animal Research Center

text: live

text: Generic

characteristic: NCBI submission package

text: Library obtained from Stratagene, catalog #937721. Library made from skeletal muscle of a two year old Holstein cow.

text: XLOLR

text: Bos taurus

text: EcoRI

text: XhoI

text: Skeletal muscle

text: MARC BSM

text: lambda

text: Uni-ZAP XR


text: The Institute of Physical and Chemical Research (RIKEN)

text: live

text: Generic

text: cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and Genome Science Laboratory in RIKEN. Division of Experimental Animal Research in Riken contributed to prepare mouse tissues. 1st strand cDNA was primed with a primer [5' GAGAGAGAGAGCGGCCGCAACTCGAGTTTTTTTTTTTTTTTTVN 3'], cDNA was prepared by using trehalose thermo-activated reverse transcriptase and subsequently enriched for full-length by cap-trapper. Second strand cDNA was prepared with the primer adapter of sequence [5' GAGAGAGAGAAGGATCCAAGAGCTCAATTAATTAATTAAACCCCCCCCCCC 3']. cDNA was cleaved with XhoI and SstI.

text: adult

text: SOLR

text: Mus musculus

characteristic: organism

text: XhoI

text: SstI

text: male

characteristic: sex

text: kidney

text: RIKEN full-length enriched, adult male kidney


text: live

text: Generic

text: adult

text: SOLR

characteristic: lab-host

text: Mus musculus

text: XhoI

text: SstI

text: male

text: lung

text: RIKEN full-length enriched, adult male lung


text: live

text: Generic

text: adult

text: SOLR

text: Mus musculus

text: XhoI

text: SstI

text: male

text: spleen

text: RIKEN full-length enriched, adult male spleen


text: live

text: Generic

text: cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and Genome Science Laboratory in RIKEN. Division of Experimental Animal Research in Riken contributed to prepare mouse tissues. 1st strand cDNA was primed with a primer [5' GAGAGAGAGAGCGGCCGCAACTCGAGTTTTTTTTTTTTTTTTVN 3'], cDNA was prepared by using trehalose thermo-activated reverse transcriptase and subsequently enriched for full-length by cap-trapper. cDNA went through one round of normalization to Rot = 5.0 and subtraction to Rot = 20.0. Second strand cDNA was prepared with the primer adapter of sequence [5' GAGAGAGAGAGCGGCCGCAATTAATTCTCGAGTTAATTAAATTAATCCCCCCCCCCC 3']. cDNA was cleaved with XhoI and SstI.

text: adult

text: SOLR

text: Mus musculus

text: XhoI

text: SstI

text: male

text: small intestine

text: RIKEN full-length enriched, adult male small intestine


text: live

text: Generic

text: cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and Genome Science Laboratory in RIKEN. Division of Experimental Animal Research in Riken contributed to prepare mouse tissues. 1st strand cDNA was primed with a primer [5' GAGAGAGAGAGCGGCCGCAACTCGAGTTTTTTTTTTTTTTTTVN 3'], cDNA was prepared by using trehalose thermo-activated reverse transcriptase and subsequently enriched for full-length by cap-trapper. Second strand cDNA was prepared with the primer adapter of sequence [5' GAGAGAGAGATTCTCGAGTTAATTAAATTAATCCCCCCCCCCCCC 3']. cDNA was cleaved with XhoI and SstI.

text: adult

text: SOLR

text: Mus musculus

text: XhoI

text: SstI

text: male

text: stomach

text: RIKEN full-length enriched, adult male stomach


text: live

text: Generic

text: cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and Genome Science Laboratory in RIKEN. Division of Experimental Animal Research in Riken contributed to prepare mouse tissues. 1st strand cDNA was primed with a primer [5' GAGAGAGAGAGCGGCCGCAACTCGAGTTTTTTTTTTTTTTTTVN 3'], cDNA was prepared by using trehalose thermo-activated reverse transcriptase and subsequently enriched for full-length by cap-trapper. Second strand cDNA was prepared with the primer adapter of sequence [5' GAGAGAGAGATTCTCGAGTTAATTAAATTAATCCCCCCCCCCCCC 3']. cDNA was cleaved with BamHI and XhoI. cDNA of size comprised between 3 and 7 Kb was selected before cloning. Vector: a modified pBluescript KS(+) after bulk excision from Lambda FLC I.

text: 1 day pregnant adult

text: Mus musculus

text: SalI

text: BamHI

text: female

text: mammary gland

text: RIKEN full-length enriched, 1 day pregnant adult female mammary gland


text: live

text: Generic

text: 10, 11 days embryo

text: SOLR

text: Mus musculus

text: XhoI

text: SstI

text: RIKEN full-length enriched, 10, 11 days embryo


text: live

text: Generic

text: cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and Genome Science Laboratory in RIKEN. Division of Experimental Animal Research in Riken contributed to prepare mouse tissues. 1st strand cDNA was primed with a primer [5' GAGAGAGAGAGCGGCCGCAACTCGAGTTTTTTTTTTTTTTTTVN 3'], cDNA was prepared by using trehalose thermo-activated reverse transcriptase and subsequently enriched for full-length by cap-trapper. cDNA went through one round of normalization to Rot = 10.0 and subtraction to Rot = 100.0. Second strand cDNA was prepared with the primer adapter of sequence [5' GAGAGAGAGATTCTCGAGTTAATTAAATTAATCCCCCCCCCCCCC 3']. cDNA was cleaved with BamHI and XhoI. Vector: a modified pBluescript KS(+) after bulk excision from Lambda FLC I.

text: adult

text: Mus musculus

text: SalI

text: BamHI

text: male

text: olfactory brain

text: RIKEN full-length enriched, adult male olfactory brain


text: Department of Zoology, National Chung Hsing University

text: live

text: Generic

text: 2-cell; embryonic day 1.5 post-fertilization

text: Mus musculus

text: ICR

characteristic: strain

text: embryo

text: Mouse ICR outbreed two-cell stage embryo cDNA


text: Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais

text: live

text: Generic

text: mRNA was extracted from lung stage worm and the library was constructed according to the manufacturer's instructions (Uni-Zap XR vector, Stratagene)

text: Lung

text: Schistosoma mansoni

text: XhoI

text: EcoRI

text: NMRI

text: Schistosoma mansoni, lung stage, pBlueScript

text: Phagemid

text: pBlueScript SK

characteristic: vector


characteristic: External Id

text: The Institute of Physical and Chemical Research (RIKEN)

text: live

text: Generic

text: cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and Genome Science Laboratory in RIKEN. Division of Experimental Animal Research in Riken contributed to prepare mouse tissues. 1st strand cDNA was primed with a primer [5' GAGAGAGAGAGCGGCCGCAACTCGAGTTTTTTTTTTTTTTTTVN 3'], cDNA was prepared by using trehalose thermo-activated reverse transcriptase and subsequently enriched for full-length by cap-trapper. Second strand cDNA was prepared with the primer adapter of sequence [5' GAGAGAGAGATTCTCGAGTTAATTAAATTAATCCCCCCCCCCCCC 3']. cDNA was cleaved with BamHI and XhoI. Vector: a modified pBluescript KS(+) after bulk excision from Lambda FLC I. Tissue was provided by William A. Held, Roswell Park Cancer Institute, Department of Molecular and Cellular Biology, Elm and Carlton Streets, Buffalo, NY 14263, whose assistance we gratefully acknowledge.

text: adult

text: Mus musculus

text: SalI

text: BamHI

text: male

text: liver tumor

characteristic: tissue-type

text: RIKEN full-length enriched, adult male liver tumor


characteristic: INSDC center name

text: live

text: Generic

text: islet cells

characteristic: cell type

text: cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and Genome Science Laboratory in RIKEN. Division of Experimental Animal Research in Riken contributed to prepare mouse tissues. 1st strand cDNA was primed with a primer [5' GAGAGAGAGAGCGGCCGCAACTCGAGTTTTTTTTTTTTTTTTVN 3'], cDNA was prepared by using trehalose thermo-activated reverse transcriptase and subsequently enriched for full-length by cap-trapper. Second strand cDNA was prepared with the primer adapter of sequence [5' GAGAGAGAGATTCTCGAGTTAATTAAATTAATCCCCCCCCCCCCC 3']. cDNA was cleaved with BamHI and XhoI. Vector: a modified pBluescript KS(+) after bulk excision from Lambda FLC I. Cells were provided by Tomohiro Kono, Department of Animal Science, Tokyo University of Agriculture, Atsugi City, Kanagawa Prefecture, Japan, whose assistance we gratefully acknowledge.

text: adult

text: Mus musculus

text: SalI

text: BamHI

text: pancreas

text: RIKEN full-length enriched, adult pancreas islet cells


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

characteristic: INSDC first public

text: live

text: Generic

text: cDNA made by oligo-dT priming. Directionally cloned using the following adaptors: 5'-TCGACCCACGCGTCCG-3' and 5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCT(15)-3'. Size selected > 1 kb for average insert length 1.9 kb. This is a primary library, non-amplified. Library constructed by Life Technologies and donated by J. Lupski, M.D./Ph.D. (Baylor College of Medicine); available through Life Technologies.

text: adult, 16 yr

text: Homo sapiens

text: NotI

text: SalI

text: male

text: sympathetic trunk

characteristic: title

text: plasmid (ampicillin resistant, 200 ug/ml)

text: pCMV-SPORT6 (Life Technologies)


characteristic: INSDC last update

text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.6 kb. Library constructed by Life Technologies.

characteristic: description

text: 5 months

text: DH10B (T1 phage-resistant)

text: Mus musculus

text: NotI

text: SalI

text: male

text: salivary gland

text: phagemid (ampicillin resistant)


text: live

characteristic: INSDC status

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.6 kb. Constructed by Life Technologies. Note: this is a NCI_CGAP Library.

text: DH10B (T1 phage-resistant)

text: colon

text: Mus musculus

text: NotI

text: SalI

text: FVB/N

text: phagemid (ampicillin resistant)


text: US Department of Agriculture, Agriculture Research Service, Pacific West Area, Western Regional Research Center

text: live

text: Generic

characteristic: NCBI submission model

text: Chinese Spring

characteristic: cultivar

text: Plants were grown under hydroponic conditions at UC Davis, salt stressed for 12 hours, and for 7 days, then dissected and frozen (Akhunov in J Dvorak Lab). Total RNA and poly(A) RNA were prepared from crown tissue, equal portions of RNA were pooled from the two treatments, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab at the University of California, Riverside (Akhunov, Chin, Choi, Close, Fenton, Kianian, Otto, Simons, Zhang). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

text: Adult plant

characteristic: dev-stage

text: E. coli SOLR

text: Triticum aestivum

text: EcoRI

text: XhoI

text: Crown

text: Wheat salt-stressed crown cDNA library

text: Phagemid

text: Lambda Uni-ZAP XR, excised phagemid


text: Beijing Institute of Heart Lung and Blood Vessel Disease

text: live

text: Generic

characteristic: NCBI submission package

text: Peripheral Blood White Cells

text: Homo sapiens

characteristic: organism

text: Male

text: Unstable Angina of AnZhen Hospital


text: Peking Union Medical College

text: live

text: Generic

text: peripheral white blood cell

text: Homo sapiens

text: Homo Sapiens cDNA Library from Peripheral White Blood Cell


text: Lawrence Livermore National Laboratory

text: live

text: Generic

text: mRNA made from carcinoma, cDNA made by oligo-dT priming. Non-directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 500 bp. Primary library. cDNA Library Preparation: David B. Krizman, Ph.D.

characteristic: lab-host

text: mouth

characteristic: organ

text: Homo sapiens

text: moderate to poorly differentiated invasive carcinoma of the retromolar trigone

text: plasmid (ampicillin resistant)


text: live

text: Generic

text: Chinese Spring

text: Plants were given a gradual stress down to 80%, 70% and to 60% RWC at Texas Tech University (D. Zhang in HT Nguyen lab). Total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab at the University of California, Riverside (Akhunov, Chin, Choi, Close, Fenton, Kianian, Otto, Simons, Zhang). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

text: Full tillering stage

text: E. coli SOLR

text: Triticum aestivum

text: EcoRI

text: XhoI

text: Leaf

text: Chinese Spring wheat drought stressed leaf cDNA library

text: Phagemid

text: Lambda Uni-ZAP XR, excised phagemid

characteristic: vector


characteristic: External Id

text: Lawrence Berkeley National Lab

text: live

text: Generic

text: The mRNA for the testis library was made from testes and seminal vessicles hand dissected from 0-3 day old Ore-R males. RNA kindly provided by the lab of Margaret Fuller. Sized fractionated cDNAs were directly ligated into pOTB7. Plasmid cDNA library.

text: 0-3 day old Ore-R males

text: Plates AT.10-AT.120: DH5-alpha. Plates AT.121-AT.319: DH5-alpha TonA

text: ADULT testes

text: Drosophila melanogaster

text: EcoRI

text: male

characteristic: sex

text: AT Drosophila melanogaster adult testes pOTB7


text: Texas A&M University

characteristic: INSDC center name

text: live

text: Generic

text: Sorghum bicolor

text: EcoRI

text: EcoRI

text: green leaf and root tissue

characteristic: tissue-type

text: Pooled green leaf and root tissue

text: pBluescript II (SK)


characteristic: INSDC first public

text: live

text: Generic

text: Epithelial

characteristic: cell type

characteristic: cell-line

text: 3' RACE of total RNA from genetrap pools; shotgun clone in pAmp-1 and used to transform DH5-alpha competent bacteria.

text: Breast

text: Homo sapiens

text: Carcinoma

text: Genetrap T47D Human Breast Carcinoma Library

text: Plasmid


characteristic: INSDC last update

text: live

text: Generic

text: NCI_CGAP_Sub8 is a subtracted library derived from NCI_CGAP_Sub5. The NCI_CGAP_Sub8 library had 2.5 million recombinants. A single-stranded DNA preparation of NCI_CGAP_Sub5 was used as a tracer in a subtractive hybridization with a driver comprising: a pool of clones from NCI_CGAP_Sub5 (IMAGE clone Ids 2732833-2737415, 3068040-3069191; 25% of the driver population), a pool of clones from NCI_CGAP_Sub4 (IMAGE clone Ids 2723592-2729326; 25% of the driver population), NCI_CGAP_Sub6 (pool AIF-AJU, IMAGE Ids 2728969-2733190; 25% of the driver population), and NCI_CGAP_Sub7 (IMAGE Ids 3069192-3072238, 3081864-3084550; 25% of the driver population). Subtraction was performed as previously described [Bonaldo, Lennon & Soares (1996): Normalization and Subtraction: Two Approaches To Facilitate Gene Discovery. Genome Research 6, 791-806.

characteristic: description

text: DH10B (Life Technologies)

text: Homo sapiens

text: Not I

text: Eco RI

characteristic: title

text: Phagemid


text: Harvard University, Howard Hughes Medical Institute

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Library constructed using SuperScript Plasmid Library kit (Life Technologies). cDNA made by oligo-dT priming.Size-selected by column fractionation; average insert size 1.06kb. Primary library, unamplified.

text: Embryonic day 16.5

text: Pancreas

text: Mus musculus

text: NotI

text: SalI

text: Both

text: ICR

characteristic: strain

text: Total pancreas

text: Melton Mouse E16 5 Pancreas Library 2 M16B2

text: plasmid (kanamycine resistant)

text: pBluescript II SK


text: Tokyo University of Marine Science and Technology, Graduate School of Marine Science and Technology

text: live

text: Generic

characteristic: NCBI submission package

text: Ig positive leukocytes

text: adult

characteristic: dev-stage

text: Paralichthys olivaceus

characteristic: organism

text: lambda ZAPII- Ig positive leukocytes


text: Institute for Plant Genetics and Crop Plant Research

text: live

text: Generic

text: Barke

characteristic: cultivar

text: mRNA was made from etiolated leaves of spring barley variety 'Barke', a high quality malting variety. Plants were grown on filterpaper for 6 d at 25 C in the dark. Leaves were harvested at daylight conditions. Sal-Adaptor Sequence: TCGACCCACGCGTCCG Average insert size is 1 kb Sequence trimming: Vector sequences and sequence ends were trimmed from the 5'-and 3'-end until a 50 bp window contains less than two ambiguities. The maximum length was set to 700 bp.

characteristic: lab-host

text: Hordeum vulgare subsp. vulgare

text: SalI

text: NotI

text: etiolated leaves

text: Hordeum vulgare Barke etiolated leaves

text: pBluescript SK-

characteristic: vector


characteristic: External Id

text: North Carolina State University

characteristic: INSDC center name

text: live

text: Generic

text: Side

text: The library is from early (spring) wood, taken from three six-year old trees (three different genotypes), in the juvenile phase. These trees were induced to form side wood by bending to a 45 degree angle and tying them to the ground. Differentiating xylem was harvested from the sides of the inclined stems, and a mixture of all three genotypes was used for the library. oligo-dT primed cDNA was directionally cloned into the EcoRI-XhoI BlueScript SK vector arms. NOTE: The sequences contain a 'cDNA adapter' between the EcoRI site and the start of the EST. The adapter sequence is 'AATTCGGCACGAG'.

text: Juvenile

text: Pinus taeda

text: Eco RI

text: XhoI

text: Coastal plain loblolly pine from North Carolina

text: Xylem

text: NXSI (Nsf Xylem Side wood Inclined)

text: BlueScript SK


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

characteristic: INSDC first public

text: live

text: Generic

text: cDNA made by oligo-dT priming. Directionally cloned into EcoRI/XhoI sites using the following 5' adaptor: GGCACGAG(G). Size-selected >500bp for average insert size 1.8kb. Library constructed by Ling Hong in the laboratory of Gerald M. Rubin (University of California, Berkeley) using ZAP-cDNA synthesis kit (Stratagene) and Superscript II RT (Life Technologies). Note: this is a NIH_MGC Library. |

text: DH10B (phage-resistant)

text: pancreas

characteristic: organ

text: Homo sapiens

text: XhoI

text: EcoRI

text: epithelioid carcinoma cell line

characteristic: tissue-type

text: plasmid (chloramphenicol resistant, grown at 30C)


characteristic: INSDC last update

text: live

text: Generic

text: cDNA made by oligo-dT priming. Directionally cloned into EcoRI/XhoI sites using the following 5' adaptor: GGCACGAG(G). Library constructed by Ling Hong in the laboratory of Gerald M. Rubin (University of California, Berkeley) using ZAP-cDNA synthesis kit (Stratagene) and Superscript II RT (Life Technologies). Note: this is a NIH_MGC Library. |

text: DH10B (phage-resistant)

text: kidney

text: Homo sapiens

text: XhoI

text: EcoRI

text: renal carcinoma (ascites)


text: University Otto von Guericke Magdeburg

text: live

text: Generic

text: Homo sapiens

text: Skin

text: Human Psoriasis Differential Display


text: live

text: Generic

text: mRNA made from thyroid carcinoma, cDNA made by oligo-dT priming. Non-directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 500 bp. Primary library. cDNA Library Preparation: David B. Krizman, Ph.D.

characteristic: description

text: thyroid

text: Homo sapiens

text: papillary carcinoma

text: plasmid (ampicillin resistant)


text: Washington University School of Medicine

text: live

text: Generic

text: The cDNA library was constructed from mRNA isolated from mature seed pods of greenhouse grown plants prior to senescence for the cultivar KP1. Complementary DNA was synthesized from mRNA using a primer consisting of a poly(dT) sequence with a XhoI restriction site. EcoRI adapters were ligated to the blunt-ended cDNA fragments followed by XhoI digestion. The cDNA fragments were directionally cloned into the EcoRI-XhoI restriction site of the pBluescript vector. The ligated cDNA fragments were transformed into DH10B host cells (GibcoBRL). This library was constructed in the laboratory of Dr. Randy Shoemaker.

text: Glycine max

text: EcoRI

text: XhoI

text: Mature seed pods, greenhouse grown

characteristic: title

text: plasmid

text: pBluescript II SK+


text: live

characteristic: INSDC status

text: Generic

text: The cDNA library was constructed from mRNA isolated from 4 day old seedling of PI468916. The seedlings were germinated in a growth chamber using germination paper. Complementary DNA was synthesized from mRNA using a primer consisting of a poly(dT) sequence with a XhoI restriction site. EcoRI adapters were ligated to the blunt-ended cDNA fragments followed by XhoI digestion. The cDNA fragments were directionally cloned into the EcoRI-XhoI restriction site of the pBluescript vector. The ligated cDNA fragments were transformed into DH10B host cells (GibcoBRL). This library was constructed in the laboratory of Dr. Randy Shoemaker.

text: Glycine soja

text: EcoRI

text: XhoI

text: Whole seedling, 4 day old

text: plasmid

text: pBluescript II SK+


text: live

text: Generic

characteristic: NCBI submission model

text: The cDNA library was constructed from mRNA isolated from degenerating cotyledons of 2 week old seedlings from PI468916. Complementary DNA was synthesized from mRNA using a primer consisting of a poly(dT) sequence with a XhoI restriction site. EcoRI adapters were ligated to the blunt-ended cDNA fragments followed by XhoI digestion. The cDNA fragments were directionally cloned into the EcoRI-XhoI restriction site of the pBluescript vector. The ligated cDNA fragments were transformed into DH10B host cells (GibcoBRL). This library was constructed in the laboratory of Dr. Randy Shoemaker.

text: Glycine soja

text: EcoRI

text: XhoI

text: Degenerating cotyledons, 2 week old seedling

text: plasmid

text: pBluescript II SK+


text: University of Liverpool

text: live

text: Generic

text: isolated from benign human mammary cell line library 123 subtracted with malignant human breast carcinoma cell line MCF-7 cDNA

text: Homo sapiens

text: breast


text: The Institute of Tropical Medicine

text: live

text: Generic

text: Several grams of Schistosoma japonicum cercaria (Hubei, P.R.China), of mixed sex, were collected after releasing from the nails(Oncomelania hupensis hupensis).Double-strain cDNA synthesized with the mRNA isolated from those cercaria was inserted into the bacteria phage lambda TriplEx2 between the SfiIA and SfiIB sites of the LacZ gene. The cDNA library was constructed by Chen Xiao-guang at First Military Medical University, Guangzhou, Guangdong, P.R.China

text: Schistosoma japonicum

text: SfiIA

text: SfiIB

text: Mix

characteristic: sex

text: Chinese mainland

characteristic: strain

text: Schistosoma japonicum cercaria Lambda TriplEx2 Express library

text: Lambda TriplEx2


text: Max-Planck-Institut fuer Molekulare Genetik

text: live

text: Generic

characteristic: NCBI submission package

text: Library preparation by oligo_dT priming of RNA. Clones can be ordered from the Resource Center in Berlin, http://www.rzpd.de.

text: embryonic 9-day

characteristic: dev-stage

text: E.coli DH10B MaxEfficiency (Gibco cells)

text: Mus musculus

text: NotI

text: SalI

text: whole embryo

text: Mouse 9-day fetus cDNA library MPMGp559

text: Plasmid


text: live

text: Generic

text: embryonic 9-day

text: E.coli, XL1 blue

text: Mus musculus

characteristic: organism

text: NotI

text: SalI

text: whole embryo

text: Mouse 9-day fetus cDNA library ICRFp522

text: Plasmid


text: University of Hong Kong

text: live

text: Generic

text: nasopharyngeal carcinoma

characteristic: cell type

characteristic: cell-line

text: Differentially expressed cDNA library of Latent membrane protein 1 expressing nasopharyngeal carcinoma cells

text: Homo sapiens


characteristic: External Id

text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: cDNAs were oligo-dT primed and directionally cloned. Library was constructed by N. Garrett, P. LeMaire, A.M. Zorn, and J.B. Gurdon (Wellcome/CRC Institute).

text: DH10B (phage-resistant)

characteristic: lab-host

text: Xenopus laevis

text: NotI

text: EcoRI

text: egg

text: Wellcome CRC pcDNAI egg

text: plasmid (ampicillin resistant)

text: pcDNAI

characteristic: vector