text: Massachusetts Institute Of Technology

text: live

text: Generic

text: Whole animal

text: ceh-30 (C33D12.7) PCR product from Shai Shaham's C. elegans cDNA library. PCR was performed using a gene-specific primer whose sequence is given in the comments of the EST sequence file and a primer specific to the vector backbone. Products were reamplified using a second gene-specific primer whose sequence is given in the comments of the EST sequence file and the same primer specific to the library vector. Products were ligated into pGEM T-Easy. Individual transformants were picked, miniprepped, and sequenced using SP6 and T7 primers. This sequence file has been trimmed to remove primer and vector sequences.

text: Whole animal

text: Caenorhabditis elegans

text: Hermaphrodite

text: Whole animal

text: Shai Shaham's C. elegans cDNA library


status: PUBLIC

characteristic: External Id

text: Tokyo University of Marine Science and Technology, Graduate School of Marine Science and Technology

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Penaeus japonicus

characteristic: organism

text: Marsupenaeus japonicus library (Hirono I)

characteristic: title


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT)-primed cDNA and cloned into pBluescript SK- (Stratagene, La Jolla, CA). Library constructed at the Kewalo Marine Laboratory, Pacific Biosciences Research Center (University of Hawaii) and sequencing was done at DOE Joint Genome Institute (Walnut Creek, CA).

characteristic: description

text: Gastrula embryo

tag: attribute

characteristic: dev-stage

text: Mnemiopsis leidyi

text: whole

characteristic: tissue-type

text: CAXH Mnemiopsis leidyi whole animal Gastrula

characteristic: vector


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: nitrogen limited. The library was made from oligo(dT)-primed cDNA and cloned into vector pDNR-LIB (Clontech Laboratories, Inc., Mountain View, CA) using the SMART system (BD Biosciences, San Jose, CA). To generate the first strand, polyA RNA was primed with an oligo(dT) primer (5'-ATTCTAGAGGCCGAGGCGGCCGACATG(T)30 VN-3', where V = A, G, or C). The second strand was synthesized by PCR using primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' followed by digestion of the SfiI sites. cDNA was size selected from 1-2kb using 1% agarose gel electrophoresis, then ligated into pDNR-LIB. Library was created and sequencing done at DOE Joint Genome Institute (Walnut Creek, CA).

text: Thalassiosira pseudonana

text: SfiI A

text: SfiI B

text: CAAC Thalassiosira pseudonana Total RNA N-

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: nitrogen limited. The library was made from oligo(dT)-primed cDNA and cloned into vector pDNR-LIB (Clontech Laboratories, Inc., Mountain View, CA) using the SMART system (BD Biosciences, San Jose, CA). To generate the first strand, polyA RNA was primed with an oligo(dT) primer (5'-ATTCTAGAGGCCGAGGCGGCCGACATG(T)30 VN-3', where V = A, G, or C). The second strand was synthesized by PCR using primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' followed by digestion of the SfiI sites. cDNA was size selected from 0.5-1kb using 1% agarose gel electrophoresis, then ligated into pDNR-LIB. Library was created and sequencing done at DOE Joint Genome Institute (Walnut Creek, CA).

text: Thalassiosira pseudonana

text: SfiI A

text: SfiI B

text: CAAG Thalassiosira pseudonana Total RNA N-

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT)-primed cDNA and cloned into vector pDNR-LIB (Clontech Laboratories, Inc., Mountain View, CA) using the SMART system (BD Biosciences, San Jose, CA). To generate the first strand, polyA RNA was primed with an oligo(dT) primer (5'-ATTCTAGAGGCCGAGGCGGCCGACATG(T)30 VN-3', where V = A, G, or C). The second strand was synthesized by PCR using primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' followed by digestion of the SfiI sites. cDNA was size selected from 0.5-1kb using 1% agarose gel electrophoresis, then ligated into pDNR-LIB. Library was created and sequencing done at DOE Joint Genome Institute (Walnut Creek, CA).

text: Thalassiosira pseudonana

text: SfiI A

text: SfiI B

text: CAAH Thalassiosira pseudonana Thaps_R2 N+ 0.5-1kb

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT)-primed cDNA and cloned into vector pDNR-LIB (Clontech Laboratories, Inc., Mountain View, CA) using the SMART system (BD Biosciences, San Jose, CA). To generate the first strand, polyA RNA was primed with an oligo(dT) primer (5'-ATTCTAGAGGCCGAGGCGGCCGACATG(T)30 VN-3', where V = A, G, or C). The second strand was synthesized by PCR using primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' followed by digestion of the SfiI sites. cDNA was size selected from 1-2kb using 1% agarose gel electrophoresis, then ligated into pDNR-LIB. Library was created and sequencing done at DOE Joint Genome Institute (Walnut Creek, CA).

text: Thalassiosira pseudonana

text: SfiI A

text: SfiI B

text: CAAI Thalassiosira pseudonana Thaps_R2 N+ 1-2kb

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: temperature limited cells (-Temp). The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 1.2-2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed at DOE Joint Genome Institute (Walnut Creek, CA).

characteristic: lab-host

text: Thalassiosira pseudonana

text: SalI

text: NotI

characteristic: strain

text: CBFN Thalassiosira pseudonana Temperature limited early stationary phase whole cells (M)


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: silicate limited cells (-Si). The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected >2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed at DOE Joint Genome Institute (Walnut Creek, CA).

text: Thalassiosira pseudonana

text: SalI

text: NotI

text: whole organism

text: CBFS Thalassiosira pseudonana silicate limited cells (-Si)


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: nitrate limited cells (-NO3). The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected >2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed at DOE Joint Genome Institute (Walnut Creek, CA).

text: Thalassiosira pseudonana

text: SalI

text: NotI

text: whole organism

text: CBFT Thalassiosira pseudonana nitrate limited cells (-NO3)


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: Iron limited cells (-Fe). The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected >2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed at DOE Joint Genome Institute (Walnut Creek, CA).

text: Thalassiosira pseudonana

text: SalI

text: NotI

text: whole organism

text: CBFU Thalassiosira pseudonana Iron limited cells (-Fe)


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: carbon dioxide limited cells (-CO2). The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected >2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed at DOE Joint Genome Institute (Walnut Creek, CA).

text: Thalassiosira pseudonana

text: SalI

text: NotI

text: whole organism

text: CBFX Thalassiosira pseudonana carbon dioxide limited cells (-CO2)


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected up to 8kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed at DOE Joint Genome Institute (Walnut Creek, CA).

text: DH10B T1R

text: Thalassiosira pseudonana

text: SalI

text: NotI

text: CBPB Thalassiosira pseudonana RNA from carbon dioxide limited cells (-CO2) 8kb


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected >2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed at DOE Joint Genome Institute (Walnut Creek, CA).

text: DH10B T1R

text: Thalassiosira pseudonana

text: SalI

text: NotI

text: CBPC Thalassiosira pseudonana Temperature limited early stationary phase whole cells


text: Norwegian College of Fishery Science, University of Tromso

text: live

text: Generic

text: Gadus morhua head-kidney normalised cDNA library after stimulation by inactivated Listonella anguillarum

text: Head-kidney

characteristic: organ

text: Gadus morhua

text: Gadus morhua head-kidney Normalised cDNA library


text: CRA, Centro ricerca genomica e postgenomica animale e vegetale

text: live

text: Generic

text: NIL3876-Rdg2a and Mirco-rdg2a

characteristic: cultivar

text: mRNAs were obtained from barley embryos of the resistant line NIL3876 and near isogenic susceptible line Mirco inoculated for 7 and 14 day with the leaf stripe pathogen Pyrenophora graminea.cDNAs differentially expressed were isolated by SSH and cDNA-AFLP analyses; differential expression of cDNA obtained by cDNA-AFLP was analysed further by micro-array. Approximately 10% of the clones correspond to cDNA from the fungus Pyrenophora graminea.

text: Hordeum vulgare

text: embryos, 7 and 14 days post inoculation with Pyrenophora graminea

text: barley embryos inoculated for 7 and 14 days with the leaf stripe pathogen


characteristic: External Id

text: Shanxi Agricultural University

text: live

text: Generic

text: Vicugna pacos

text: female

characteristic: sex

text: skin

text: ASCD

text: lambdaTriplex


text: China Agricultural University

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: Inbred line S1

text: PCR-select cDNA subtraction was performed using a Clontech kit according to established protocols. The cDNA from treated seedlings with 4h low light was used as a tester and the cDNA from untreated seedlings used as a driver. The cDNAs were digested with RsaI and then ligated to different adapters. Two rounds of hybridization and PCR amplification were processed to normalize and enrich the differentially expressed cDNAs. Products of the secondary PCR from the forward subtraction were directly inserted into pGEMB. T-easy Vector (Promega) and transformed into E. coli DH5N1 cells. All the recombinant clones were picked up to establish the subtracted cDNA library.

text: After the emergence of the three primary leaves

text: Cucumis sativus

text: Leaves

text: Low-light stress specific subtracted cDNA library of cucumber seedlings

text: pGEMB. T-easy Vector (Promega)


text: Defence Agricultural Research Laboratory

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Ladakh Ecotype

text: Lepidium latifolium

text: Leaf

text: Lepidium cold responsive cDNA subtraction Library


text: Shiraz University

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Seedlings of Alvand, a cold tolerant wheat were grown in growth chamber for 16 days. Isolation was carried out from leaves and by specific primers via PCR.

text: Leaf

text: Triticum aestivum

text: Alvand

text: Triticum aestivum Alvand 16 days seedlings under normal condition


text: Shiraz University

text: live

text: Generic

text: Seedlings of Bayat bread wheat were grown in growth chamber for 16 days. Isolation was carried out from leaves and by specific primers via PCR.

text: Leaf

text: Triticum aestivum

characteristic: organism

text: Bayat

text: Triticum aestivum Bayat 16 days seedlings under normal condition

characteristic: title


text: Shiraz University

text: live

text: Generic

text: Seedlings of Darab1 wheat were grown in growth chamber for 16 days. Isolation was carried out from leaves and by specific primers via PCR.

characteristic: description

characteristic: dev-stage

text: Leaf

text: Triticum aestivum

text: Triticum aestivum Darab1 16 days seedlings under normal condition


text: Shiraz University

text: live

text: Generic

text: Seedlings of Omid wheat were grown in growth chamber for 16 days. Isolation was carried out from leaves and by specific primers via PCR.

text: Leaf

text: Triticum aestivum

text: Omid

text: Triticum aestivum Omid 16 days seedlings under normal condition


text: Shiraz University

text: live

text: Generic

text: Seedlings of Sardadi wheat were grown in growth chamber for 16 days. Isolation was carried out from leaves and by specific primers via PCR.

text: Leaf

text: Triticum aestivum

text: Sardari

text: Triticum aestivum Sardari 16 days seedlings under normal condition


text: Shiraz University

text: live

text: Generic

text: Seedlings of Shahi, a cold tolerant wheat were grown in growth chamber for 16 days. Isolation was carried out from leaves and by specific primers via PCR.

text: Leaf

text: Triticum aestivum

text: Shahi

text: Triticum aestivum Shahi 16 days seedlings under normal condition


text: Shiraz University

text: live

text: Generic

text: Seedlings of Zarrin wheat were grown in growth chamber for 16 days. Isolation was carried out from leaves and by specific primers via PCR.

text: Leaf

text: Triticum aestivum

text: Zarrin

text: Triticum aestivum Zarrin 16 days seedlings under normal condition


text: National Institute of Genetics

text: live

text: Generic

text: 1st strand cDNA was primed with an oligo (dT) primer [GCGGCTGAAGACGGCCTATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded cDNA was ligated to a DraIII adaptor [GGCCUACUGG], digested and directionally cloned into distinct DraIII sites of the pME18S- FL3. Library was size selected for 1.0 kb, with a average insert size of 1.2kb. Library constructed by Yutaka Suzuki (University of Tokyo, Institute of Medical Science). Custom primers recommended for sequencing: 5' end primer 5'-GGATGTTGCCTTTACTTCTA-3' and 3' end primer 5'-CGACCTGCAGCTCGAGCACA-3'.

text: Monosiga ovata

text: DraIII

text: DraIII

text: whole cell

characteristic: tissue-type

text: Full length cDNA Library, Monosiga ovata, MOC

characteristic: vector


text: National Institute of Agrobiological Sciences

text: live

text: Generic

text: 5 weeks

text: Sus scrofa

text: male

text: main and accessory cerebella

text: full-length enriched pig cDNA library, main and accessory cerebella


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT)-primed cDNA and cloned into the pMCL200_cDNA vector. PolyA RNA was primed with oligo(dT) primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'-CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 0.6-1.2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pMCL200_cDNA vector. Library was created and sequencing done by the DOE Joint Genome Institute (Walnut Creek, CA).

text: Adult

characteristic: lab-host

text: Lottia gigantea

text: SalI

text: NotI

text: head

text: CAWC Lottia gigantea from head (L)


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT)-primed cDNA and cloned into the pMCL200_cDNA vector. PolyA RNA was primed with oligo(dT) primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'-CGGACGCGTGGG) and digested with NotI. cDNA was size selected >2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pMCL200_cDNA vector. Library was created and sequencing done by the DOE Joint Genome Institute (Walnut Creek, CA).

text: Adult

text: Lottia gigantea

text: SalI

text: NotI

text: mantle

text: CAWF Lottia gigantea from mantle (H)


text: DOE Joint Genome Institute

text: live

text: Generic

text: Adult

text: Lottia gigantea

text: SalI

text: NotI

text: mantle

text: CAWG Lottia gigantea from mantle (L)


text: DOE Joint Genome Institute

text: live

text: Generic

text: Adult

text: Lottia gigantea

text: SalI

text: NotI

text: foot

text: CAWH Lottia gigantea from foot (H)


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT)-primed cDNA and cloned into the pMCL200_cDNA vector. PolyA RNA was primed with oligo(dT) primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'-CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 2.5-8kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pMCL200_cDNA vector. Library was created and sequencing done by the DOE Joint Genome Institute (Walnut Creek, CA).

text: Adult

text: Lottia gigantea

text: SalI

text: NotI

text: mixture of head, foot, Visceral mass (Radula), and ctenidia (heart)

text: CAXP Lottia gigantea from head, foot, radula and heart


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT)-primed cDNA and cloned into the pMCL200_cDNA vector. PolyA RNA was primed with oligo(dT) primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'-CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 1-2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pMCL200_cDNA vector. Library was created and sequencing done by the DOE Joint Genome Institute (Walnut Creek, CA).

text: Adult

text: Lottia gigantea

text: SalI

text: NotI

text: CAXS Lottia gigantea from head, foot, radula and heart


text: DOE Joint Genome Institute

text: live

text: Generic

text: Adult

text: Lottia gigantea

text: SalI

text: NotI

text: mantle

text: CAXT Lottia gigantea from mantle


text: DOE Joint Genome Institute

text: live

text: Generic

text: Adult

text: Lottia gigantea

text: SalI

text: NotI

text: female gonad

text: CAXU Lottia gigantea from female gonad


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT)-primed cDNA and cloned into the pMCL200_cDNA vector. PolyA RNA was primed with oligo(dT) primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'-CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 1.5-3kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pMCL200_cDNA vector. Library was created and sequencing done by the DOE Joint Genome Institute (Walnut Creek, CA).

text: Adult

text: Lottia gigantea

text: SalI

text: NotI

text: female gonad

text: CAXW Lottia gigantea from female gonad


text: DOE Joint Genome Institute

text: live

text: Generic

text: Adult

text: Lottia gigantea

text: SalI

text: NotI

text: male gonad

text: CAXX Lottia gigantea from male gonad


text: DOE Joint Genome Institute

text: live

text: Generic

text: Adult

text: Lottia gigantea

text: SalI

text: NotI

text: male gonad

text: CAXY Lottia gigantea from male gonad


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 0.5-2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed at DOE Joint Genome Institute (Walnut Creek, CA).

text: 12h, 15h, 18h

text: Lottia gigantea

text: SalI

text: NotI

text: embryos

text: CBBG Lottia gigantea 12,15,18h embryos


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 1.2-2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed at DOE Joint Genome Institute (Walnut Creek, CA).

text: Lottia gigantea

text: SalI

text: NotI

text: Larvae

text: CBBH Lottia gigantea 26h,37h,61h Larvae (M)


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 0.6-1.2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed at DOE Joint Genome Institute (Walnut Creek, CA).

text: Lottia gigantea

text: SalI

text: NotI

text: Larvae

text: CBBI Lottia gigantea 26h,37h,61h Larvae (L)


text: DOE Joint Genome Institute

text: live

text: Generic

text: Lottia gigantea

text: SalI

text: NotI

text: Larvae

text: CBBN Lottia gigantea 3,4,5,6.5d Larvae (M)


text: DOE Joint Genome Institute

text: live

text: Generic

text: Lottia gigantea

text: SalI

text: NotI

text: embryos

text: CBGB Lottia gigantea 3,6,9,12h embryos


text: DOE Joint Genome Institute

text: live

text: Generic

text: 15h 18h

text: Lottia gigantea

text: SalI

text: NotI

text: embryos

text: CBGC Lottia gigantea 15h 18h embryos


text: Institute of Himalayan Bioresource Technology

text: live

text: Generic

text: Leaf

characteristic: organ

text: Rheum australe

text: Leaf

text: Rheum australe reverse (control; 25C) suppression subtractive hybridization (SSH) library

text: plasmid

text: pGEM-T Easy (Promega)


text: Bioengineering College, Chongqing University

text: live

text: Generic

text: The subtractive hybridization protocol from BD Bioscience Clontech (Palo Alto, CA) has been used to generate this specific cDNA library. Tester cDNA was prepared from microcycle conidiation cultures and driver cDNA was prepared from conidiation mycelium. The resulting cDNAs were cloned into PMD-18T vector.

text: Metarhizium acridum

characteristic: strain

text: microcycle conidiation SSH Library


characteristic: External Id

text: Medical center of Tongji University

text: live

text: Generic

text: lung

text: Rattus norvegicus

text: GTAC

text: CATG

text: Rat lung cancer produced by nickel library

text: plasmid

text: pEGM T vector


text: Medical center of Tongji University

text: live

text: Generic

text: lung

text: Rattus norvegicus

text: GTAC

text: CATG

text: Rattus lung tissue library

text: plasmid

text: pEGM T vector


text: University of Liverpool

text: live

text: Generic

text: S ratti mixed stage

text: mixed

text: Strongyloides ratti

text: S. ratti mixed stage pAMP

text: plasmid

text: pAMP


text: University of Liverpool

text: live

text: Generic

text: S ratti mixed stage

text: mixed

text: Strongyloides ratti

text: S. ratti mixed stage TOPO

text: plasmid

text: TOPO


text: Genoscope - Centre National de Sequencage

text: live

text: Generic

text: INRA 717-1-B4

characteristic: cultivar

text: A cDNA library was made with mRNA isolated from young differentiating xylem from tension wood side harvested on 1-year-old poplar trees. The sampling was done on 2 different tilted trees grown in the greenhouse. cDNAs were cloned in an oriented way into SfiI (A and B) restriction sites. A one-step conversion of Lambda TriplEx2 to the corresponding pTriplEx2 plasmid was done via site-specific recombination at loxP sites (Clontech ; SMART cDNA library construction kit).

text: 1-year-old poplar trees grown in the greenhouse

text: Populus tremula x Populus alba

text: female

characteristic: sex

text: Young differentiating xylem from tension wood side harvested on one-year-old poplar submitted to a drought stress


characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: INRA 717-1-B4

text: A cDNA library was made with mRNA isolated from young differentiating xylem from opposite wood side harvested on 1-year-old poplar trees. The sampling was done on 2 different tilted trees grown in the greenhouse. cDNAs were cloned in an oriented way into SfiI (A and B) restriction sites. A one-step conversion of Lambda TriplEx2 to the corresponding pTriplEx2 plasmid was done via site-specific recombination at loxP sites (Clontech ; SMART cDNA library construction kit).

text: Populus tremula x Populus alba

text: female

text: Young differentiating xylem from opposite wood side harvested on one-year-old poplar submitted to a drought stress


text: Justus-Liebig University of Giessen

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: subtracted cDNA library. Zurich strain originally isolated by Pierre Tardent from Lake Zurich, Switzerland

text: adult

text: Hydra vulgaris

text: Zurich strain

text: whole animals, Immune challenged

text: Hydra vulgaris Zurich strain subtracted cDNA library

text: pGEM-T Easy


text: Justus-Liebig University of Giessen

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: subtracted cDNA library.

text: adult

text: Schmidtea mediterranea

text: asexual strain originated from a fountain in Montjuic, Barcelona, Spain

text: whole animals, Immune challenged

text: Schmidtea mediterranea Montjuic strain subtracted cDNA library

text: pGEM-T Easy


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 1.2-2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed and sequencing performed at the DOE Joint Genome Institute (Walnut Creek, CA).

text: DH10B T1 resistant

text: Metridium senile

characteristic: organism

text: SalI

text: NotI

text: siphonoglyph

text: CBHH Metridium senile siphonglyph

characteristic: title


text: DOE Joint Genome Institute

text: live

text: Generic

characteristic: description

text: DH10B T1 resistant

text: Metridium senile

text: SalI

text: NotI

text: tentacle

text: CBHI Metridium senile tentacle


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected >2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed and sequencing performed at the DOE Joint Genome Institute (Walnut Creek, CA).

text: DH10B T1 resistant

text: Metridium senile

text: SalI

text: NotI

text: tentacle

characteristic: tissue-type

text: CBHN Metridium senile tentacle


text: Instituto de Biologia Molecular y Celular de Plantas (Universidad Politecnica de Valencia - Consejo Superior de Investigaciones Cientificas)

text: live

text: Generic

text: Navelina

text: cDNA library made from poly-A+ RNA from leaves of plants grown in greenhouse conditions, and subjected to a prolonged salt treatment: regular irrigations (three times a week) with 25 mM NaCl:CaCl2 solution for 60 days

text: adult trees

characteristic: dev-stage

text: Escherichia coli

text: leaves

text: Citrus sinensis

text: hermaphrodite

text: Leaves

text: SLH

text: Plasmid

text: Lambda ZAP

characteristic: vector


text: University of New Mexico

text: live

text: Generic

text: mRNA, pooled from 20 M-line B. glabrata snails, exposed to Schistosoma haematobium (12 hours), was reverse transcribed with poly-T priming and used to generate a directional cDNA library (Uni-ZAP XR, Stratagene) with an average insert size estimated at ~1 kb. Inserts from randomly excised pBluescript II KS (+) phagemids were sequenced single pass using an M13 primer. camhe = C Adema M line Haematobium EST. Vector and linker adapters were removed from sequence as presented.

text: adult

text: Biomphalaria glabrata

text: hermaphrodite

text: M-line

text: whole body

text: CAMHE

text: Plasmid


text: University of New Mexico

text: live

text: Generic

text: mRNA, pooled from 20 M-line B. glabrata snails, exposed to Schistosoma mansoni (12 hours), was reverse transcribed with poly-T priming and used to generate a directional cDNA library (Uni-ZAP XR, Stratagene) with an average insert size estimated at ~1 kb. Inserts from randomly excised pBluescript II KS (+) phagemids were sequenced single pass using the M13(-20) primer. camme = C Adema M line Mansoni EST. Vector and linker adapters were removed from sequence as presented.

text: adult

text: Biomphalaria glabrata

text: hermaphrodite

text: M-line

text: whole body

text: CAMME

text: Plasmid

text: pBluescript II KS (+)


text: University of New Mexico

text: live

text: Generic

text: mRNA, pooled from 20 M-line B. glabrata snails, injected with Escherichia coli (12 hours), was reverse transcribed with poly-T priming and used to generate a directional cDNA library (Uni-ZAP XR, Stratagene) with an average insert size estimated at ~1 kb. Inserts from randomly excised pBluescript II KS (+) phagemids were sequenced single pass using an M13 primer. camne = C Adema M line Gram Negative EST. Vector and linker adapters were removed from sequence as presented.

text: adult

text: Biomphalaria glabrata

text: hermaphrodite

text: M-line

text: whole body

text: CAMNE

text: Plasmid

text: pBluescript II KS (+)


text: University of New Mexico

text: live

text: Generic

text: mRNA, pooled from 20 M-line B. glabrata snails, injected with Micrococcus luteus (12 hours), was reverse transcribed with poly-T priming and used to generate a directional cDNA library (Uni-ZAP XR, Stratagene) with an average insert size estimated at ~1 kb. Inserts from randomly excised pBluescript II KS (+) phagemids were sequenced single pass using an M13 primer. campe = C Adema M line Gram Positive EST. Vector and linker adapters were removed from sequence as presented.

text: adult

text: Biomphalaria glabrata

text: hermaphrodite

text: M-line

text: whole body

text: CAMPE

text: Plasmid

text: pBluescript II KS (+)


text: Suzhou University

text: live

text: Generic

text: Neoporphyra haitanensis

text: Porphyra haitanensis cDNA


text: Institute of Botany,Chinese Academy of Sciences

text: live

text: Generic

text: callus

characteristic: cell type

text: Saussurea medusa cell cultures are cultured in Murashige and Skoog (1962) supplemented with 3% sucrose, 2 mg/l C1 NAA and 0.5 mg/l C1 6-BA 1% agar under control conditions as well as subjected to cold (0-4C), stress for 3, 6, 9, 12 d. cDNA Library was constructed using total RNA from cold stressed cell cultures. Total RNAs from different treatments were extracted and equal amounts were pooled before mRNA isolation. First cDNA synthesis was conducted by coverting poly(A)+RNA to double - stranded cDNA by using primer: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-d(T)30N C1N-3'(N = A, G, C, or T; N C1 = A, G, or C)and 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3'. Double stranded cDNAs were amplified LD PCR with primers containing SfiIA and SfiIB. The purified PCR products, representing the entire cloned cDNA population, were used as a driver for normalization. The normalized cDNAs were digested and then cloned into SfiIA and SfiIB digested pDNR-LIB plasmid vector (Clontech). The primary library had a titer of 2.5 x 106 pfu/ml in which 98% clones were recombinants and the insert cDNAs were from 0.5 kb to 3 kb in length. Screening of the cDNA Library by PCR using primers of M13F:5'-GTAAAACGACGGCCAGT-3' and M13R:5'-AAACAGCTATGACCATGTTCA-3'.

text: cell cultures of 3,6,9,12 days

text: Saussurea medusa

text: SfiIA

text: SfiIB

text: Cell cultures of Saussurea medusa

text: Plasmid

text: pDNR-LIB


text: Kyungpook National University

text: live

text: Generic

text: adult stage

text: Bombus terrestris

text: male

text: brain

text: Brain of the adult queen of Bombus terrestris


text: Fujian Agriculture and Forestry University

text: live

text: Generic

text: Dimocarpus longan

text: leaves stressed with simulated acid rain of pH2.5

text: Longan acid rain stressed library


text: live

text: Generic

text: Pop-DG

text: Full-length cDNA library made from a mix of poly-A+ RNA from mesocarp of peach fruits stored at 5 degrees for different times, and showing symptoms of chilling injury.

text: adult trees

text: Escherichia coli

characteristic: lab-host

text: fruits

characteristic: organ

text: Prunus persica

text: mesocarp


characteristic: External Id

text: live

text: Generic

text: Pop-DG

characteristic: cultivar

text: Full-length, normalized cDNA library made from a mix of poly-A+ RNA from mesocarp of peach fruits stored at 5 degress for different times, and showing symptoms of chilling injury.

text: adult trees

text: Escherichia coli

text: fruits

text: Prunus persica

text: mesocarp

text: PPN


characteristic: INSDC center name

text: live

text: Generic

text: Cleopatra

text: cDNA library made from a mixture of equal amounts of poly-A+ RNA from plants subjected to the following abiotic stresses: 1) water stress, 2) wates stress and rehydration, 3) salt stress, 4) progressive salt stress, and 5) iron chlorosis

text: adult trees

text: Escherichia coli

text: roots

text: Citrus reshni

text: hermaphrodite

characteristic: sex

text: Plasmid


characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Navelina

text: cDNA library made from poly-A+ RNA from the abscission zone C and surrounding tissues of mature fruits from plants grown with normal culture practices

text: adult trees

text: Escherichia coli

text: fruits

text: Citrus sinensis

text: hermaphrodite

text: Plasmid


text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Cidro Etrog

text: Full-length cDNA library made from poly-A+ RNA from leaves of plants infected with Citrus Exocortis viroid (CEVd)

text: adult trees

text: Escherichia coli

text: leaves

text: Citrus medica

text: hermaphrodite

text: Plasmid


text: live

text: Generic

characteristic: NCBI submission package

text: Full-length cDNA library made from poly-A+ RNA from leaves of plants infected with Citrus Tristeza virus (CTV)

text: adult trees

text: Escherichia coli

text: leaves

text: Citrus macrophylla

characteristic: organism

text: hermaphrodite

text: Plasmid


text: Instituto de Biologia Molecular y Celular de Plantas (Universidad Politecnica de Valencia - Consejo Superior de Investigaciones Cientificas)

text: live

text: Generic

text: Fortune

text: Subtracted cDNA library made from poly-A+ RNA from flavedo of stored fruits heat-treated at 37 degrees for 3 days and cooled at 1.5 degrees for 7 days (tester) and freshly harvested fruits (driver)

characteristic: description

text: adult trees

text: Escherichia coli

text: fruits

text: Citrus clementina x Citrus tangerina

text: hermaphrodite

text: flavedo

text: FlavCurFrS

characteristic: title

text: Plasmid


text: live

text: Generic

text: Full-length cDNA library made from poly-A+ RNA from leaves of plants infected with HSVd viroid

text: adult trees

characteristic: dev-stage

text: Escherichia coli

text: leaves

text: Citrus macrophylla

text: hermaphrodite

text: Plasmid


text: live

text: Generic

text: Cleopatra

text: cDNA library made from a mix of poly-A+ RNA from roots of plants growing in greenhouse conditions subjected to KCl 50 mM, or KCl 100 mM, or short term Cl- starvation, or long term Cl- starvation, or Cl- supply after Cl- starvation

text: adult trees

text: Escherichia coli

text: roots

text: Citrus reshni

text: hermaphrodite

text: Plasmid

characteristic: vector


text: live

text: Generic

text: Cleopatra

text: cDNA library made from a mix of poly-A+ RNA from roots inoculated with Phytophthora citrophtora zoospores by inmersion in a 9000 or 90000 zoospores/ml suspension, and sampled at 0, 4, and 8 days after inoculation

text: adult trees

text: Escherichia coli

text: roots

text: Citrus reshni

text: hermaphrodite

text: Plasmid

text: Uni-ZAP XR


text: Instituto de Agroquimica y Tecnologia de Alimentos (IATA-CSIC)

text: live

text: Generic

text: Navelina

text: Subtracted cDNA library made from poly-A+ RNA from the rind (flavedo + albedo) of mature fruits wound-inoculated with Penicillium digitatum (1000000 spores/mL) and then stored at 20oC for 24 hours

text: adult trees

text: Escherichia coli

text: fruits

text: Citrus sinensis

text: hermaphrodite

text: flavedo and albedo

characteristic: tissue-type

text: RindPdigS

text: Plasmid

text: pBluescript


text: live

text: Generic

text: Cleopatra

text: Full-length, normalized cDNA library made from a mix of poly-A+ RNA from roots of plants subjected to different stresses: water stress, water stress and rehydration, salt stress, progressive salt stress, iron deficiency, and Phytophthora citrophtora fungus infection

text: adult trees

text: Escherichia coli

characteristic: lab-host

text: roots

text: Citrus reshni

text: hermaphrodite

text: StrCleopN

text: Plasmid


text: International Center for Tropical Agriculture (CIAT)

text: live

text: Generic

text: This library was constructed from several development stages and tissues. The development stage and tissue of each entry are unknown.

text: Manihot esculenta

text: mixture of leaf, root

text: full-length enriched cassava cDNA library


text: Emerging Technologies, QDPI&F

text: live

text: Generic

text: country: Australia:North Queensland; variety: Tainung 5

text: Carica papaya

text: bisexual

text: fruit mesocarp and skin

text: Carica papaya Tainung 5 fruit mesocarp and skin


text: Institute of Life Sciences

text: live

text: Generic

text: Suppressive subtraction hybridization was performed using the SuperSMART cDNA Synthesis Kit and the Clontech PCR-Select cDNA Subtraction Kit taking the mRNA from the leaf tissue of salt (425 mM) treated plants as Tester and that from control (receiving no salt treatment) as Driver. The secondary PCR product was non-directionally cloned in a pGEM-T easy vector (Promega) using PCR Cloning Kit. The EST clones were sequenced using the SP6 Forward primer at TCGA (The Centre for Genomic Application), New Delhi.

text: 2 months plant exposed to 425 mM Salt (NaCl) for 24 hours

text: Suaeda maritima

text: ECoRI

text: ECoRI

text: Leaf

text: Suaeda maritima 425 mM salt stress (NaCl) SSH Library

text: Plasmid

text: pGEM-T easy


text: Novozymes

text: live

text: Generic

text: Aspergillus oryzae

characteristic: strain

text: Aspergillus oryzae EST Library


characteristic: External Id

text: live

text: Generic

text: Larva molting from 5th to 6th instar

text: Epidermis

characteristic: organ

text: Choristoneura fumiferana

text: EcorR I

text: Xho I

text: Epidermis

text: Spruce Budworm Epidermis Library

text: Plasmid

text: pBluescript SK


text: live

text: Generic

text: Larva molting from 5th to 6th instar

text: Fat Body

text: Choristoneura fumiferana

text: EcorR I

text: Xho I

text: Fat Body

text: Spruce Budworm Fat Body Library

text: Plasmid

text: pBluescript SK


text: live

text: Generic

text: Larva molting from 5th to 6th instar

text: Midgut

text: Choristoneura fumiferana

text: EcorR I

text: Xho I

text: Midgut

text: Spruce Budworm Midgut Library

text: Plasmid

text: pBluescript SK


text: Auburn University

characteristic: INSDC center name

text: live

text: Generic

text: peripheral blood leukocytes

characteristic: cell type

characteristic: cell-line

text: Ictalurus punctatus

text: Sal I

text: Not I

text: CBCZ: cDNA library made from Channel catfish mixed leukocyte culture MLC52-1(IpCGJx13)

text: Phagemid


text: Auburn University

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: mixed tissues from stomach, muscle, olfactory tissue, and trunk kidney

text: Ictalurus furcatus

text: Sfi IA

text: Sfi IB

text: CBFH: Normalized blue catfish cDNA library of stomach, muscle, olfactory tissue, and trunk kidney (mixed tissue 4, AUJ)

text: Phagemid

text: pDNR-Lib


text: Auburn University

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: mixed tissues from head kidney, liver, spleen, gill, skin and intestine

text: Ictalurus punctatus

text: Sfi IA

text: Sfi IB

text: CBNG: Normalized channel catfish cDNA library from head kidney, gill, intestine, spleen, skin and liver (mixed issue 1, AUK)

text: Phagemid

text: pDNR-Lib


text: Auburn University

text: live

text: Generic

characteristic: NCBI submission package

text: Ictalurus furcatus

characteristic: organism

text: Sfi IA

text: Sfi IB

text: CBNH: Normalized blue catfish cDNA library from head kidney, liver, spleen, gill, skin and intestine (mixed tissue 3, AUE)

text: Phagemid

text: pDNR-Lib


text: Auburn University

text: live

text: Generic

text: mixed tissues from stomach, muscle, olfactory tissue and trunk kidney

text: Ictalurus punctatus

text: Sfi IA

text: Sfi IB

text: CBNI: Normalized channel catfish cDNA library from stomach, muscle, olfactory tissue and trunk kidney (mixed tissue 2, AUL)

characteristic: title

text: Phagemid

text: pDNR-Lib


text: Auburn University

text: live

text: Generic

text: Peripheral blood leukocytes

text: Ictalurus punctatus

text: Sfi IA

text: Sfi IB

text: CBPO: Normalized channel catfish cDNA library from LPS-stimulated peripheral blood leukocytes (JxLPS1)

text: Phagemid

text: pDNR-Lib


text: Auburn University

text: live

text: Generic

text: Ictalurus punctatus

text: Sfi IA

text: Sfi IB

text: CBZB: Normalized channel catfish cDNA library from head kidney, gill, intestine, spleen, skin and liver (mixed tissue 1, AUK_M)

text: Phagemid

text: pDNR-Lib


text: Auburn University

text: live

text: Generic

text: Ictalurus furcatus

text: Sfi IA

text: Sfi IB

text: CBZC: Normalized blue catfish cDNA library from stomach, muscle, olfactory tissue and trunk kidney (mixed tissue 4, AUJ_M)

text: Phagemid

text: pDNR-Lib


text: Auburn University

text: live

text: Generic

text: whole fish

text: Ictalurus punctatus

text: Sal I

text: Not I

text: CBFA: Normalized cDNA library made from whole fry of 5-, 9-, and 14-day old channel catfish (Fry 5)

text: Phagemid


text: Auburn University

text: live

text: Generic

text: mixed tissues from liver, pituitary, ovary and testes of three-year-old fish

text: Ictalurus punctatus

text: Sfi IA

text: Sfi IB

text: CBPN: Subtracted channel catfish cDNA library from liver, pituitary, ovary and testes (Mixed tissue library 5, MTL5)

text: Phagemid

text: pDNR-Lib


text: Auburn University

text: live

text: Generic

text: Ictalurus punctatus

text: Sfi IA

text: Sfi IB

text: CBZA: Normalized channel catfish cDNA library from stomach, muscle, olfactory tissue and trunk kidney (mixed tissue 2, AUL_M)

text: Phagemid

text: pDNR-Lib


text: Auburn University

text: live

text: Generic

text: Ictalurus furcatus

text: Sfi IA

text: Sfi IB

text: CBZF: Normalized blue catfish cDNA library from head kidney, gill, intestine, spleen, skin and liver (mixed tissue 3, AUE_M)

text: Phagemid

text: pDNR-Lib


text: live

text: Generic

text: Female-subtract-Male 3-dpc. embryonic cDNA library, BD biosciences Clontech (cat. #K1804-1)

characteristic: description

text: 3 days post-conception embryo

characteristic: dev-stage

text: Gallus gallus

text: White Leghorn

text: Chicken Female-subtract-Male 3-dpc. embryonic cDNA library


text: University of Alberta

text: live

text: Generic

text: 5 years old

text: ElectroMAX DH10B T1 Phage-Resistant Cells

text: Liver

text: Odocoileus virginianus

text: Sal I (5' end of cDNA)

text: Not I (3' end of cDNA)

text: Male

characteristic: sex

text: Liver

characteristic: tissue-type

text: Deer Liver Full-Length cDNA Library

text: pCMV SPORT 6.0

characteristic: vector


characteristic: External Id

text: University of Alberta

text: live

text: Generic

characteristic: lab-host

text: Mammary Gland

text: Bos taurus

text: Sal I (5' end of cDNA)

text: Not I (3' end of cDNA)

text: Mammary Gland

text: Bovine Mammary Gland Subtract Full-Length cDNA Library

text: pCMV SPORT 6.0


text: National Diagnostics Centre

characteristic: INSDC center name

text: live

text: Generic

text: Suppression subtractive hybridization library

text: april smolt vs may smolt

text: Salmo salar

text: Gill

text: Salmo salar Gill SSH forward


text: Dipartimento di Ortofloroarboricoltura e Tecnologie Agroalimentari, Sezione Arboricoltura, University of Catania,

text: live

text: Generic

text: Total RNA was extracted using Plant RNA Reagent (Invitrogen) from flowers at different developmental stages, sampled from three genotypes differing for sex (male, female, hermaphrodite). Equal amount of total RNAs were bulked before mRNA isolation. mRNA was isolated by Oligotex mRNA mini Kit (Qiagen). A cDNA library was constructed using cDNA Synthesis Kit and Uni-ZAP XR vector (Stratagene) following the manufacturer's instructions. After the excision of pBluescript SK(-) phagemid from the Uni-ZAP XR through ExAssist helper phage, and the infection of E. coli SOLR cells, the colonies were plated on LB medium with 100 ug/ml ampicillin. Clones were randomly picked for bidirectional sequencing with T3 and T7 primers. Clones were sequenced using an ABI 3730 XL sequencer (Applied Biosystems) at the Institute for Integrative Genome Biology, Core Instrumentation Facilities (CIF), University of California, Riverside (UCR).

text: E. coli SOLR

text: Ceratonia siliqua

text: EcoRI

text: XhoI

text: Ceratonia siliqua flowers cDNA library

text: phagemid

text: Lambda UNi-ZAP XR


text: Kanazawa University

text: live

text: Generic

text: type strain of Bombyx mori

text: 5th instar larva day 2

text: Bombyx mori

text: Kinshu x Showa

characteristic: strain

text: brain

text: V2 brain 20-hydroxyecdysone-induced cDNA subtraction library


text: Kanazawa Univ Sch Med

text: live

text: Generic

text: Carassius auratus

text: scale without the lateral line

text: subtraction library, the scale without lateral line minus the gill


text: Kanazawa Univ Sch Med

text: live

text: Generic

text: Carassius auratus

text: gill

text: subtraction library, the gill minus the scale without lateral line


text: Kanazawa Univ Sch Med

text: live

text: Generic

text: Carassius auratus

text: scale with the lateral line

text: subtraction library, the scale with lateral line minus the scale without lateral line


text: Pontificia Universidad Catolica de Chile

text: live

text: Generic

text: Ulva compressa exposed to copper

text: Ulva compressa

text: Vegetative thallus

text: Ulva compressa copper Library


text: J. Craig Venter Institute (JCVI; www.jcvi.org)

text: live

text: Generic

text: oocyst

text: Cryptosporidium muris RN66

text: Cryptosporidium muris (strain RN66) cDNA library, stage oocyst


text: Brock University

text: live

text: Generic

text: Catharanthus roseus

text: CrUniGene leaf


text: Brock University

text: live

text: Generic

text: Catharanthus roseus

text: CrUniGene root


text: Brock University

text: live

text: Generic

text: Catharanthus roseus

text: CrUniGene stem


text: MPI for Molecular Genetics

text: live

text: Generic

text: Mytilus edulis


text: MPI for Molecular Genetics

text: live

text: Generic

text: Mytilus edulis

text: mytilus


text: National Diagnostics Centre

text: live

text: Generic

text: parr vs precocious parr

text: Salmo salar

text: Brain

text: Salmo salar Brain SSH forward


text: National Diagnostics Centre

text: live

text: Generic

text: parr vs precocious parr

text: Salmo salar

text: Brain

text: Salmo salar Brain SSH Reverse


text: National Diagnostics Centre

text: live

text: Generic

text: smolt april vs smolt may

text: Salmo salar

text: Gill

text: Salmo salar Gill SSH reverse


text: National Diagnostics Centre

text: live

text: Generic

text: smolt april vs smolt may

text: Salmo salar

text: Head Kidney

text: Salmo salar Head Kidney SSH Forward


text: National Diagnostics Centre

text: live

text: Generic

text: smolt april vs smolt may

text: Salmo salar

text: Head Kidney

text: Salmo salar Head Kidney SSH Reverse


text: National Diagnostics Centre

text: live

text: Generic

text: feb parr vs april parr

text: Salmo salar

text: Hypothalamus

text: Salmo salar Hypothalamus SSH forward feb parr vs april parr


text: National Diagnostics Centre

text: live

text: Generic

text: feb parr vs april parr

text: Salmo salar

text: Hypothalamus

text: Salmo salar Hypothalamus SSH reverse feb parr vs april parr


text: National Diagnostics Centre

text: live

text: Generic

text: parr vs mature adult female

text: Salmo salar

text: Hypothalamus

text: Salmo salar Hypothalamus SSH forward parr vs mature adult female


text: National Diagnostics Centre

text: live

text: Generic

text: parr vs mature adult female

text: Salmo salar

text: Hypothalamus

text: Salmo salar Hypothalamus SSH reverse parr vs mature adult female


text: National Diagnostics Centre

text: live

text: Generic

text: parr vs precocious parr

text: Salmo salar

text: Hypothalamus

text: Salmo salar Hypothalamus SSH forward parr vs precocious parr


text: National Diagnostics Centre

text: live

text: Generic

text: parr vs precocious parr

text: Salmo salar

text: Hypothalamus

text: Salmo salar Hypothalamus SSH reverse parr vs precocious parr


text: National Diagnostics Centre

text: live

text: Generic

text: smolt april vs smolt may

text: Salmo salar

text: Intestine

text: Salmo salar Intestine SSH Forward


text: National Diagnostics Centre

text: live

text: Generic

text: smolt april vs smolt may

text: Salmo salar

text: Intestine

text: Salmo salar Intestine SSH Reverse


text: National Diagnostics Centre

text: live

text: Generic

text: smolt april vs smolt may

text: Salmo salar

text: Spleen

text: Salmo salar Spleen SSH Forward


text: National Diagnostics Centre

text: live

text: Generic

text: smolt april vs smolt may

text: Salmo salar

text: Spleen

text: Salmo salar Spleen SSH Reverse


text: University of Georgia

text: live

text: Generic

text: National Tropical Botanic Garden

characteristic: cultivar

text: The library was prepared from polyA+ RNA from premeiotic flowerbuds. Only floral buds with diameter of 2 mm of less were used for RNA isolation. Double-stranded cDNA was unidirectionally cloned into pBluescript SK- (Stratagene) with the 5' end of the cDNA inserted at the EcoRI site and the 3' polyA+ inserted at Xho1 site. A library-specific adaptor (GGCACGAGG) was used to verify the library identity and then trimmed along with vector and low quality sequence. cDNA Library Statistics: Avg. insert length: 1611; Primary Titer: 2.24E6 pfu total; Amplified Titer: 1.37E10 pfu/ml; Antibiotic: 50 ug/ml Ampicillin. This library was been generated by the Floral Genome Project (FGP; http://fgp.bio.psu.edu) and additional sequencing was done as part of the Ancestral Angiosperm Project (www.AncestralAngiosperm.org). Both projects are funded by NSF's Plant Genome Research Program (DBI-0115684 and DBI-0638595, respectively).

text: premeiotic

text: SOLR (E. Coli)

text: premeiotic flower buds

characteristic: organ

text: Amborella trichopoda

text: Male

text: flower buds

text: FGP Male Amborella trichopoda

text: Plasmid


text: University of Georgia

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: National Tropical Botanic Garden_F

text: The library was prepared from polyA+ RNA from premeiotic flowerbuds. Only floral buds with diameter of 2.5 mm of less were used for RNA isolation. Double-stranded cDNA was unidirectionally cloned into pBluescript SK- (Stratagene) with the 5' end of the cDNA inserted at the EcoRI site and the 3' polyA+ inserted at Xho1 site. A library-specific adaptor (GGGTCCTGG) was used to verify the library identity and then trimmed along with vector and low quality sequence. cDNA Library Statistics: Avg. insert length: 1031; Primary Titer: 5E6 pfu total; Amplified Titer: 1.4E10 pfu/ml; Antibiotic: 50 ug/ml Ampicillin. This library was been generated by the Floral Genome Project (FGP; http://fgp.bio.psu.edu) and additional sequencing was done as part of the Ancestral Angiosperm Project (www.AncestralAngiosperm.org). Both projects are funded by NSF's Plant Genome Research Program (DBI-0115684 and DBI-0638595, respectively).

text: premeiotic

text: SOLR (E. Coli)

text: premeiotic flower buds

text: Amborella trichopoda

text: Female

text: flower buds

text: FGP Female Amborella trichopoda

text: Plasmid


text: Liaoning Normal University

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Rana chensinensis

text: skin

text: Chinese forest frog skin cDNA library

text: Plasmid


text: National Centre for Plant Genome Research

text: live

text: Generic

characteristic: NCBI submission package

text: Suppression Subtractive Hybridisation

text: Cicer arietinum

characteristic: organism

text: Shoot

text: Chickpea Ascochyta-Induced Library


text: United States Department of Agriculture-Agriculture Research Service

text: live

text: Generic

text: Total RNA isolated from Haematobia irritans egg

characteristic: description

text: egg

text: DH10B E. coli bacterial cells, T1 phage resistant

text: Haematobia irritans irritans

text: NotI

text: ecoRV

text: Male and Female

text: irritans

text: egg

text: Haematobia irritans eggs

characteristic: title

text: Plasmid

text: pExpress 1


text: live

text: Generic

text: Total RNA isolated from Haematobia irritans 1st Instar Larvae.

text: 1st Instar Larvae

characteristic: dev-stage

text: Haematobia irritans irritans

text: NotI

text: ecoRV

text: Male and Female

characteristic: sex

text: irritans

text: 1st Instar Larvae

text: Haematobia irritans 1st Instar Larvae

text: Plasmid

text: pExpress 1


text: live

text: Generic

text: Natural Killer cell

characteristic: cell type

text: Homo sapiens

text: Human Natural Killer cell cDNA


characteristic: External Id

text: live

text: Generic

text: subtracted cDNA library by suppression subtractive hybridization with Clontech SMART PCR cDNA Synthesis Kit. This fragment came from knee joint cartilage of Yong New Zealand white rabbit.

text: Oryctolagus cuniculus

text: New Zealand White

text: knee joint cartilage

characteristic: tissue-type

text: rabbit knee joint cartilage


text: live

text: Generic

text: Ofanto

text: Suppression-subtractive hybridization was performed using the PCR-SelectTM cDNA Subtraction Kit (Clontech) according to the manufacturer's recommendations. Approximately 100ng PCR-amplified cDNA were ligated without further purification into 50ng vector and cloned into pGEM-T Easy vector (Promega).

text: Booting

text: E. coli

characteristic: lab-host

text: Flag leaves

text: Triticum turgidum subsp. durum

text: EcoRI

text: durum

characteristic: sub species

text: BOT

text: Plasmid

text: pGem-T easy

characteristic: vector


text: live

text: Generic

text: Ofanto

characteristic: cultivar

text: Dough stage

text: E. coli

text: Seeds

characteristic: organ

text: Triticum turgidum subsp. durum

text: EcoRI

text: durum

text: CER

text: Plasmid

text: pGem-T easy


characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Ofanto

text: Milk stage

text: E. coli

text: Seeds

text: Triticum turgidum subsp. durum

text: EcoRI

text: durum

text: LAT

text: Plasmid

text: pGem-T easy


text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Ofanto

text: 3 leaves on moist paper

text: E. coli

text: Roots

text: Triticum turgidum subsp. durum

text: EcoRI

text: durum

text: RDA

text: Plasmid

text: pGem-T easy


text: live

text: Generic

characteristic: NCBI submission package

text: Ofanto

text: 3 leaves on moist paper

text: E. coli

text: Roots

text: Triticum turgidum subsp. durum

characteristic: organism

text: EcoRI

text: durum

text: RDB

text: Plasmid

text: pGem-T easy


text: live

text: Generic

text: Ofanto

characteristic: description

text: Flowering

text: E. coli

text: Flag leaves

text: Triticum turgidum subsp. durum

text: EcoRI

text: durum

characteristic: title

text: Plasmid

text: pGem-T easy


text: live

text: Generic

text: Ofanto

text: 3 leaves on moist paper

characteristic: dev-stage

text: E. coli

text: Leaves

text: Triticum turgidum subsp. durum

text: EcoRI

text: durum

text: Plasmid

text: pGem-T easy


text: Massachusetts Institute of Technology

characteristic: INSDC center name

text: live

text: Generic

text: Ps-n cells were harvested during late exponential to mid-stationary growth phase, under predominantly domoic-acid-producing conditions. First-strand cDNA was prepared from 5 B5g poly (A) + RNA using Superscript II, NC-p7, and oligo pd(TZ). Double-stranded cDNA was generated using RNase H, E. coli DNA polymerase I, and E. coli ligase. The ends of the cDNAs were polished with T4 DNA polymerase and GstXI adaptors were ligated to the cDNA ends. Ps-n cDNA was then fractionated on sucrose gradients, ligated into pMD1 (a pUC-based vector) and transformed, by electroporation, into E. coli DH10B cells (Das et al., 2001). T7 forward (TAATACGACTCACTATAGGG) and M13 reverse (CAGGAAACAGCTATGAC) flanked the cloning site of the pMD1 vector.

text: Ps-n clone CL-125

characteristic: isolate

text: Pseudo-nitzschia multiseries

text: Ps-n cDNA Library

text: plasmid


characteristic: External Id

text: Central Tuber Crops Research Institute

text: live

text: Generic

text: Colocasia esculenta infected with Phytophthora colocasiae (leaf blight fungus) after seven days

text: Plant infected with P.colocasiae

text: Leaf

text: Colocasia esculenta

text: Leaf

characteristic: tissue-type

text: Colocasia esculenta infected with Phytophthora colocasiae after seven days

text: pGEM-T


text: Root Biology Center, South China Agricultural University

text: live

text: Generic

text: Soybean genotype BX10 was hydroponically grown in the greenhouse with an average temperature of 28/19C (day/night) and relative humidity ranging from 50%-80%. One week later, the seedlings were transplanted into one-half-strength modified Hoagland solution containing 250 uM KH2PO4. The seedlings were grown for ten days till the first trifolia were fully developed. Furthermore, plants treated with 0.2 uM Pi (1000 uM Pi as control) for 0.5, 1, 6, and 12 h were used as the materials for construction of short-term Pi starvation-induced cDNA libraries, while plants treated with 0.2 uM Pi (1000 uM Pi as control) for 3, 6, 9, and 12 d were used as the materials for construction of long-term Pi starvation-induced cDNA libraries, respectively. The cDNAs generated from plants at low P level (0.2 uM Pi) served as testers, while cDNAs generated from plants at high P level (1000 uM Pi) were used as drivers. Totally three SSH libraries were constructed, including a root short-term library (RS), a root long-term library (RL), and a shoot long-term library (SL). Correspondingly, RNA from shoots and roots subjected to Pi starvation at 0.5, 1, 6 and 12 h was separately extracted and pooled for construction of root short-term library and a shoot short-term library, while RNA from shoots and roots subjected to Pi starvation at 3, 6, 9 and 12 d was separately extracted and pooled for construction of root long-term library and a shoot long-term library.

text: Glycine max

text: shoot

text: Glycine max cv. BX10 shoot long-term (SL) SSH library

text: pGem-T easy


text: live

text: Generic

text: Glycine max

text: root

text: Glycine max cv. BX10 root short-term (RS) SSH library

text: pGem-T easy


text: live

text: Generic

text: Glycine max

text: root

text: Glycine max cv. BX10 root long-term (RL) SSH library

text: pGem-T easy


text: Oklahoma State University

text: live

text: Generic

text: Ixodes scapularis

text: female

characteristic: sex

text: pooled guts and salivary glands

text: subolesin dsRNA and saline injected Ixodes scapularis SSH Library


text: Auburn University

text: live

text: Generic

text: alate female whole body

text: Solenopsis invicta

text: EcoR I

text: Xho I

text: Fire Ant cDNA library

text: Plasmid

text: pBluescript SK

characteristic: vector


text: Institute of Hydrobiology, Chinese Academy of Sciences

text: live

text: Generic

text: blastulae embryonic cells

characteristic: cell type

text: Carassius auratus

text: Crucian carp blastulae embryonic cells library


text: Washington University School of Medicine

text: live

text: Generic

text: The library was constructed by Claire Murphy, Brandi Chiapelli, and Dr. James McCarter at Washington University, St. Louis. Oligo(dT)-SL1 PCR based library. Ancylostoma caninum L3s recovered from the tissues of mice that had been infected with A. caninum infective L3s (L3s at 10-14 days post infection are considered to be 'tissue arrested' stages) was used for cDNA library construction. cDNA PCR products of size >400 nucleotides containing SL1 on the 5' end and oligo(dT) on the 3' end were non-directionally cloned into pCRII-TOPO(Invitrogen) following the Topo TA cloning protocol. Nematodes were provided by Dr. Prema Arasu (Prema_Arasu@ncsu.edu) of North Carolina State University in Raleigh, NC.

text: tissue-arrested L3

characteristic: lab-host

text: Ancylostoma caninum

text: EcoRI

text: EcoRI

text: Ancylostoma caninum L3 SL1 TOPO v1 Murphy Chiapelli

text: plasmid (kanamycin and ampicillin resistant)

text: pCRII-TOPO (Invitrogen)


text: College of Life Scineces, Wuhan University, P.R.China; Institute of Hydrobiology, P.R.China

characteristic: INSDC first public

text: live

text: Generic

text: In this SSH library, the RNA from fertilized embryos at dome stage was used as 'tester' and the RNA from kidney cell nuclear transplantation embryos was used as 'driver'.

text: Blastula Dome

text: Danio rerio

text: AB

characteristic: strain

text: Fertilized embryos at Blastula

text: Plasmid


characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Blastula Dome

text: Danio rerio

text: AB

text: Somatic cell cloned embryos at Blastula

text: Plasmid


text: University of Georgia

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Clone 108

characteristic: cultivar

text: The library was prepared from polyA+ RNA from premeiotic flowerbuds. Only floral buds with diameter of 35 mm of less were harvested from a single genotype for RNA isolation. Double-stranded cDNA was unidirectionally cloned into pBluescript SK- (Stratagene) with the 5' end of the cDNA inserted at the EcoRI site and the 3' polyA+ inserted at Xho1 site. A library-specific adaptor (GCTGGCTGG) was used to verify the library identity and then trimmed along with vector and low quality sequence. cDNA Library Statistics: Avg. insert length: 1346; Primary Titer: 3E6 pfu total; Amplified Titer: 2E10 pfu/ml; Antibiotic: 50 ug/ml Ampicillin. This library was been generated by the Floral Genome Project (FGP; http://fgp.bio.psu.edu) and additional sequencing was done as part of the Ancestral Angiosperm Project (www.AncestralAngiosperm.org). Both projects are funded by NSF's Plant Genome Research Program (DBI-0115684 and DBI-0638595, respectively).

text: premeiotic

text: SOLR (E. Coli)

text: Premeiotic lower buds

characteristic: organ

text: Liriodendron tulipifera

text: flower buds

text: Liriodendron Flower Bud Library

text: Plasmid


text: University of Georgia

text: live

text: Generic

text: UF McCarty Hall tree

text: The library was prepared from polyA+ RNA from premeiotic flowerbuds. Only floral buds with diameter of 2.5 mm of less were used for RNA isolation. Double-stranded cDNA was unidirectionally cloned into pBluescript SK- (Stratagene) with the 5' end of the cDNA inserted at the EcoRI site and the 3' polyA+ inserted at Xho1 site. A library-specific adaptor (GCGTGTGGG) was used to verify the library identity and then trimmed along with vector and low quality sequence. cDNA Library Statistics: Avg. insert length: 1349; Primary Titer: 2.74E6 pfu total; Amplified Titer: 2.57E10 pfu/ml; Antibiotic: 50 ug/ml Ampicillin. This library was been generated by the Floral Genome Project (FGP; http://fgp.bio.psu.edu) and additional sequencing was done as part of the Ancestral Angiosperm Project (www.AncestralAngiosperm.org). Both projects are funded by NSF's Plant Genome Research Program (DBI-0115684 and DBI-0638595, respectively).

characteristic: description

text: premeiotic

characteristic: dev-stage

text: SOLR (E. Coli)

text: Flower buds

text: Persea americana

characteristic: organism

text: flower buds

text: Persea americana flower bud library

characteristic: title

text: Plasmid


characteristic: External Id

text: Texas A&M University-Corpus Christi

characteristic: INSDC center name

text: live

text: Generic

text: Four week old seedlings incubated in simulated acid mine drainage for four days. Total RNA was reverse-transcribed and used for Differential Display experiment. Sequence represents putatively induced transcript cloned from gel band.

text: Seedlings

text: Typha latifolia

text: Typha latifolia Differential Display - AMD


text: University of Natural Resources and Life Sciences

text: live

text: Generic

text: strain was induced in MA medium with paraquat (oxidative stress)

text: Pyricularia grisea

text: MA829 Cooke (Pyricularia grisea)

text: mycelium (dried)

characteristic: tissue-type

text: Mycelium of Pyricularia grisea induced with paraquat and control


text: Department of Ecology & Evolutionary Biology, University of Toronto

text: live

text: Generic

text: C. brenneri mixed life stages, both male and female

text: mixed

text: Caenorhabditis brenneri

text: male and female

characteristic: sex

text: lambda

text: lambda TriplEx2

characteristic: vector


characteristic: INSDC first public

text: live

text: Generic

text: C. japonica mixed life stages, both male and female

text: mixed

text: Caenorhabditis japonica

text: male and female

text: lambda

text: lambda TriplEx2


text: live

text: Generic

text: C. sp. 5 mixed life stages, both male and female

text: mixed

text: Caenorhabditis sinica

text: male and female

characteristic: strain

text: lambda

text: lambda TriplEx2


text: Tottori University

characteristic: INSDC last update

text: live

text: Generic

text: exons were cloned into pBluescriptII SK- at the SalI and BamHI sites, and proliferated in E.coli XL1-Blue. exons were isolated from human chromosome 11q23 specific cosmids using a modification of the method of exon amplification (Proc. Natl. Acad. Sci. USA 88: 4005-4009,1991)

text: E.coli XL1-Blue

characteristic: lab-host

text: Homo sapiens

text: SalI

text: BamHI

text: Human chromosome 11q23 mRNA (M.Katoh)

text: pBluescriptII SK-


text: Children's Memorial Research Center

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: The CMRC-CP-II1 library is a normalized library (derived from the UI-CP-II0 library) which was normalized according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CCTGAATAG. Tissue was obtained from the Provasoli-Guillard National Center for Culture of Marine Phytoplankton (CCMP).

text: DH10B T1 phage resistant (Invitrogen)

text: Cyanophora paradoxa

text: EcoR I

text: Not I

text: Plasmid

text: pT7T3-Pac (Pharmacia) with a modified polylinker


text: INRA - BDR

text: live

text: Generic

characteristic: NCBI submission package

text: distribution : CRB GADIE, crb-gadie@jouy.inra.fr, INRA, CEA Radiobiologie et Etude du genome (LREG), Domaine de Vilvert, 78352, Jouy-en-Josas cedex, FRANCE, +33 (0) 1.34.65.29.14, +33 (0 ) 1.34.65.28.02.

text: 55 days of gestation

text: Ovis aries

text: fetal ovary

text: ovine foetal ovary tissues (Prophase I meiosis) -M (ocaj)

text: pGEMT easy


text: INRA - BDR

text: live

text: Generic

text: 82 days of gestation

text: Ovis aries

text: fetal ovary

text: ovine foetal ovary tissues ( Early folliculogenesis) - F (ocai)

text: pGEMT easy


text: NewBioTechnic, S.A. (NBT)

text: live

text: Generic

text: Trichoderma atroviride

text: IMI 352941

text: mycelium Lambda Zap Library L09


text: live

text: Generic

text: Trichoderma atroviride

text: CECT 2413

text: mycelium Lambda Zap Library L12 (Trichoderma atroviride CECT 2413)


text: NewBioTechnic, S.A. (NBT)

text: live

text: Generic

text: Trichoderma atroviride

text: IMI 352941

text: mycelium Lambda Zap Library L12 (Trichoderma atroviride IMI 352941)


text: NewBioTechnic, S.A. (NBT)

text: live

text: Generic

text: Trichoderma lixii

text: CECT 2413

text: mycelium Lambda Zap Library L12 (Hypocrea lixii CECT 2413)


text: NewBioTechnic, S.A. (NBT)

text: live

text: Generic

text: Trichoderma atroviride

text: IMI 352941

text: mycelium Lambda Zap Library L13


text: NewBioTechnic, S.A. (NBT)

text: live

text: Generic

text: Trichoderma atroviride

text: IMI 352941

text: mycelium Lambda Zap Library L22


text: NewBioTechnic, S.A. (NBT)

text: live

text: Generic

text: Trichoderma aggressivum

text: IBT 9394

text: mycelium Lambda Zap Library L50


text: NewBioTechnic, S.A. (NBT)

text: live

text: Generic

text: Trichoderma atroviride

text: ATCC 74058

text: mycelium Lambda Zap Library L51


text: NewBioTechnic, S.A. (NBT)

text: live

text: Generic

text: Trichoderma lixii

text: IBT 9385

text: mycelium Lambda Zap Library L52


text: NewBioTechnic, S.A. (NBT)

text: live

text: Generic

text: Trichoderma atroviride

text: ATCC 74058

text: mycelium Lambda Zap Library L53


text: NewBioTechnic, S.A. (NBT)

text: live

text: Generic

text: Trichoderma atroviride

text: ATCC 74058

text: mycelium Lambda Zap Library L54


text: NewBioTechnic, S.A. (NBT)

text: live

text: Generic

text: Trichoderma stromaticum

text: CBS 100875

text: mycelium Lambda Zap Library L55


text: NewBioTechnic, S.A. (NBT)

text: live

text: Generic

text: Trichoderma stromaticum

text: CBS 100875

text: mycelium Lambda Zap Library L56


text: NewBioTechnic, S.A. (NBT)

text: live

text: Generic

text: Trichoderma stromaticum

text: CBS 100875

text: mycelium Lambda Zap Library L57


text: NewBioTechnic, S.A. (NBT)

text: live

text: Generic

text: Trichoderma lixii

text: UNINA A6

text: mycelium Lambda Zap Library L58


text: NewBioTechnic, S.A. (NBT)

text: live

text: Generic

text: Trichoderma lixii

text: ATCC 20847

text: mycelium Lambda Zap Library L59


text: NewBioTechnic, S.A. (NBT)

text: live

text: Generic

text: Trichoderma lixii

text: CECT 2413

text: mycelium Lambda Zap Library L60


text: NewBioTechnic, S.A. (NBT)

text: live

text: Generic

text: Trichoderma atroviride

text: IMI 352941

text: mycelium Lambda Zap Library L61


text: Shanghai Institute of Veterinary Research, Chinese Academy of Agricultural Sciences

text: live

text: Generic

text: Total RNA was extracted from 44d adult worms of Schistosoma japonicum by Trizol reagent and mRNA was isolated from the total RNA. Then, ds cDNA was synthesized by reverse transcription using random primers. After that, directional EcoRI/HindIII linkers were ligated into the ends of ds cDNA and then digested, so two blunt ends EcoRI and HindIII were formed. The digested ds cDNA fragments longer than 300bp in length were fractionated, and then ligated into T7 Select 10-3b vector. After packaging in vitro, the T7 Select 10-3b vector was transformed into BLT5403 to construct the T7 phage display cDNA library.

text: Adult worm 44d

text: Schistosoma japonicum

text: Chinese mainland strain

text: Schistosoma japonicum 44d adult worm phage display cDNA library

text: Phage

text: T7 Select 10-3b vector


text: Leibniz Institute of Plant Genetics and Crop Plant Research (IPK)

text: live

text: Generic

text: Ingrid BC mlo-5

characteristic: cultivar

text: Hordeum vulgare

text: RUS


text: live

text: Generic

text: Barke

text: developing caryopsis, 8-15 DAP(days after pollination). Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.

characteristic: description

text: 8-15 DAP (days after pollination)

text: Hordeum vulgare subsp. vulgare

text: EcoRI (5'-end of cDNA)

text: XhoI (3'-end of cDNA)

text: developing caryopsis

text: HB (Hordeum vulgare cv. Barke)

text: pBluescript SK+


text: live

text: Generic

text: Barke

text: mRNA was made from developing caryopsis (3.-15.DAP) of spring barley variety 'Barke', a high quality malting variety. Cloning sites: EcoRI (5'-end of cDNA) and XhoI (3'-end of cDNA). NOTE: Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRI adapter. Average insert size is 1 kb Sequence trimming: Vector sequences and sequence ends were trimmed from the 5'-and 3'-end until a 50 bp window contains less than two ambiguities. The maximum length was set to 700bp

text: developing caryopsis (3.-15.DAP)

characteristic: dev-stage

text: XLOLR

text: Hordeum vulgare subsp. vulgare

text: EcoRI

text: XhoI

text: developing caryopsis

text: HY

text: pBK-CMV


text: IRD (Institut de Recherche pour le Developpement)

text: live

text: Generic

text: Bourbon

text: Clontech PCR-select cDNA subtraction library; Bourbon-specific (Bourbon as tester and Laurina as driver in the subtraction process)

text: E.coli

text: leaf

characteristic: organ

text: Coffea arabica

characteristic: organism

text: Coffea arabica var Laurina-Bourbon SSH library

text: PGEM-T


characteristic: External Id

text: University of Natural Resources and Life Sciences

text: live

text: Generic

text: strain was induced in MA medium with paraquat (oxidative stress)

text: Fusarium graminearum

text: mycelium (dried)

text: Mycelium of Fusarium graminearum induced with paraquat

characteristic: title


text: Lawrence Berkeley National Lab

characteristic: INSDC center name

text: live

text: Generic

text: Sequences of RNA Ligase Mediated Rapid Amplification of cDNA Ends (RLM-RACE) products representing 5' ends of selected transcripts. Total RNA isolated from mixed stage embryos of the 'iso-1' isogenic strain was provided by the laboratory of Peter Cherbas (University of Indiana, Bloomington). Gene-specific 5' RLM-RACE products were generated using the FirstChoice RLM-RACE procedure (Ambion). Vector sequences were removed and the RNA adapter sequence was used to determine the orientation of the clone and was removed from the sequence. Each sequence represents a potential transcription start site and is oriented along the direction of transcription. NOTE: clones are not preserved and are not available for distribution.

text: mixed-stage embryo

text: whole organism

text: Drosophila melanogaster

text: male and female

text: RM Drosophila melanogaster mixed embryo RACE


text: National Institute of Animal Science (Korea)

text: live

text: Generic

text: Cap trapping method

text: 5 weeks to 25 weeks

text: Abdomen

text: Sus scrofa

text: Crossbred (Yorkshire and Landrace)

text: Abdominal fat

characteristic: tissue-type

text: SUSFLECK Abdominal Fat Normalized


text: live

text: Generic

text: Cap trapping method

text: 5 weeks to 25 weeks

text: Abdomen

text: Sus scrofa

text: Crossbred (Yorkshire and Landrace)

text: Abdominal fat

text: SUSFLECK Abdominal Fat

characteristic: vector


characteristic: INSDC first public

text: live

text: Generic

text: preadipocyte and differentiated adipocyte

characteristic: cell type

text: Cap trapping method

text: pooling of cells 0 day, 5 days and 10 days after induction of differentiation into adipocyte

text: Sus scrofa

text: Crossbred (Yorkshire and Landrace)

text: SUSFLECK Fat Cell Normalized


characteristic: INSDC last update

text: live

text: Generic

text: Cap trapping method

text: Sus scrofa

text: Crossbred (Yorkshire and Landrace)

characteristic: strain

text: SUSFLECK Fat Cell


text: live

characteristic: INSDC status

text: Generic

text: Cap trapping method

text: 5 weeks to 25 weeks

characteristic: lab-host

text: loin muscle

text: Sus scrofa

text: Crossbred (Yorkshire and Landrace)

text: longissimus dorsi

text: SUSFLECK Loin Muscle Normalized


text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Cap trapping method

text: 5 weeks to 25 weeks

text: loin muscle

text: Sus scrofa

text: Crossbred (Yorkshire and Landrace)

text: longissimus dorsi

text: SUSFLECK Loin Muscle


text: live

text: Generic

text: Cap trapping method

characteristic: description

text: 5 weeks to 25 weeks

text: liver

text: Sus scrofa

text: Crossbred (Yorkshire and Landrace)

text: liver

text: SUSFLECK Liver Normalized


text: live

text: Generic

text: Cap trapping method

text: 5 weeks to 25 weeks

characteristic: dev-stage

text: liver

text: Sus scrofa

text: Crossbred (Yorkshire and Landrace)

text: liver

text: SUSFLECK Liver


text: live

text: Generic

text: Cap trapping method

text: 5 weeks to 25 weeks

text: pituitary gland

characteristic: organ

text: Sus scrofa

text: Crossbred (Yorkshire and Landrace)

text: whole pituitary gland

text: SUSFLECK Pituitary Gland Normalized


text: live

text: Generic

text: Cap trapping method

text: 5 weeks to 25 weeks

text: pituitary gland

text: Sus scrofa

characteristic: organism

text: Crossbred (Yorkshire and Landrace)

text: whole pituitary gland

text: SUSFLECK Pituitary Gland


characteristic: External Id

text: INRA

text: live

text: Generic

text: Library was made using Suppression Subtractive Hybridization method (Diatchenko et al., 1996) from white roots harvested on 1 year old cuttings after 6 hours of hypoxia. A mixture of genotypes was used.

text: white roots on one year old cuttings

text: Quercus robur

text: root

text: Quercus robur SSH1

text: pGMT Easy


text: INRA

text: live

text: Generic

text: white roots on one year old cuttings

text: Quercus petraea

text: root

text: Quercus petraea SSH2

characteristic: title

text: pGMT Easy


text: INRA

characteristic: INSDC center name

text: live

text: Generic

text: Library was made using Suppression Subtractive Hybridization method (Diatchenko et al., 1996) from white roots harvested on 1 year old cuttings after 48 hours of hypoxia. A mixture of genotypes was used.

text: white roots on one year old cuttings

text: Quercus petraea

text: root

text: Quercus petraea SSH4

text: pGMT Easy


text: INRA

text: live

text: Generic

text: white roots on one year old cuttings

text: Quercus robur

text: root

characteristic: tissue-type

text: Quercus robur SSH3

text: pGMT Easy


text: The University of Georgia

text: live

text: Generic

characteristic: cultivar

text: cDNA libraries were constructed from Limnanthes alba OMF164, an open-pollinated population. Total RNAs from immature embryos were isolated with Trizol (Invitrogen). The cDNAs above 500 bp were used for cDNA library construction. cDNA libraries were constructed with Smart cDNA Library Construction Kit (Clontech). The inserts were directionally cloned into Clontech pDNR-LIB vector after Sfi I digestion. The clones were transformed to the host cell DH10B and selected by antibiotic chloramphenicol.

text: Limnanthes alba

text: SfiIA (GGCCATTACGGCC)

text: SfiIB (GGCCGAGGCGGCC)

text: Immature embryo

text: Limnanthes alba EST Library MF1

text: Plasmid

text: pDNR-LIB (Clontech)

characteristic: vector


text: Washington University School of Medicine

characteristic: INSDC first public

text: live

text: Generic

text: Whole embryos, mixed developmental stages

text: TransforMax EC100-T1

text: Priapulus caudatus

text: NotI

text: EcorI

text: Mixed

characteristic: sex

text: plasmid

text: pBluescript II SK+


text: Wuhan University

characteristic: INSDC last update

text: live

text: Generic

text: Adult

text: Isometrus maculatus

text: Venom gland

text: Isometrus maculatus venom gland Library


text: Brock University

text: live

characteristic: INSDC status

text: Generic

text: Little Delicata

text: Catharanthus roseus


text: National Institute of Agrobiological Sciences

text: live

text: Generic

text: isolation_source: rice seedlings ; country: Japan: Shimane, Izumo ; collection_date: 1987

text: adult 0-5 day

text: Nilaparvata lugens

text: female

text: Izumo

characteristic: strain

text: midgut

text: NLMA


text: live

text: Generic

characteristic: NCBI submission model

text: adult 4-5 day

text: Nilaparvata lugens

text: female

text: Izumo

text: ovary

text: NLOC


text: live

text: Generic

characteristic: NCBI submission package

text: adult 0-5 day

text: Nilaparvata lugens

text: female

text: Izumo

text: salivary glands

text: NLSG


text: live

text: Generic

characteristic: description

text: adult 0-1 day

text: Nilaparvata lugens

text: male

text: Izumo

text: testis

text: NLTA


text: live

text: Generic

text: adult 0-1 day

characteristic: dev-stage

text: Nilaparvata lugens

text: female

text: Izumo

text: thorax

text: NLTH


text: live

text: Generic

text: 3-5 day

text: Nilaparvata lugens

text: Izumo

text: egg

text: NLEB


text: live

text: Generic

text: 0-1.5 day

text: Nilaparvata lugens

text: Izumo

text: egg

text: NLEA


text: live

text: Generic

text: adult 0-5 day

text: Nilaparvata lugens

text: male

text: Izumo

text: midgut

text: NLMB


text: live

text: Generic

text: 1st-instar nymph

text: Nilaparvata lugens

text: Izumo

text: whole body

text: NLNA


text: live

text: Generic

text: adult 0-1 day

text: Nilaparvata lugens

text: female

text: Izumo

text: ovary

text: NLOA


text: live

text: Generic

text: adult 0-1 day

text: Nilaparvata lugens

text: male

text: Izumo

text: abdomen

text: NLAD


text: live

text: Generic

text: adult 0-1 day

text: Nilaparvata lugens

text: female

text: Izumo

text: head

text: NLHA


text: live

text: Generic

text: adult 0-1 day

text: Nilaparvata lugens

text: male

text: Izumo

text: mixture of head, thorax

text: NLHT


text: live

text: Generic

text: 4th-instar nymph

text: Nilaparvata lugens

text: Izumo

text: whole body

text: NLNB


text: live

text: Generic

text: adult 0-1 day

text: Nilaparvata lugens

text: female

text: Izumo

text: abdomen

text: NLAA


text: live

text: Generic

text: adult 0-1 day

text: Nilaparvata lugens

text: male

text: Izumo

text: abdomen

text: NLAC


text: live

text: Generic

text: adult 0-1 day

text: Nilaparvata lugens

text: female

text: Izumo

text: abdomen

text: NLAB


text: live

text: Generic

text: adult 4-5 day

text: Nilaparvata lugens

text: female

text: Izumo

text: ovary

text: NLOB


text: University of Padova

text: live

text: Generic

text: mRNA was extracted from two strains of Aspergillus carbonarius antagonists for Ochratoxin A production. A cDNA-AFLP differential display was performed with EcoRI/MseI restriction enzymes, and with 88 different combinations of Eco/Mse primers.

text: 14-day old liquid culture

text: Aspergillus carbonarius

text: mycelia

text: Aspergillus carbonarius cDNA-AFLP library


text: University of Agricultural Sciences

text: live

text: Generic

text: Eleusine coracana 20 days old seedlings were subjected to different levels of water deficit stress by withholding water. The water status was monitored gravimetrically until it reaches the prescribed level and leaf tissue was collected for total RNA isolation. RNA from different stress levels was pooled and library was made using Stratagene ZAP-cDNA synthesis kit.

characteristic: lab-host

text: Eleusine coracana

text: EcoRI

text: XhoI

text: leaf

text: Eleusine coracana water deficit stress induced cDNA library

text: Phage

text: pBlueScript


text: FNC-CENICAFE, Centro Nacional de Investigaciones de Cafe

text: live

text: Generic

text: Treatment, Larvae feeding coffee beans

text: Second instar larvae

text: E. coli

text: Hypothenemus hampei

characteristic: organism

text: Sfi I A site

text: Sfi I B site

text: Midgut

text: Plasmid

text: pDNR-Lib (Clontech)


characteristic: External Id

text: National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency

text: live

text: Generic

text: The forward subtractive library of cDNA in testes of broodstock and juvenile shrimp

text: E. coli JM109

text: Testes

characteristic: organ

text: Penaeus monodon

text: Male

text: Shrimp Testis SSH Forward Library

text: Plasmid

text: pGEM-T Easy


characteristic: INSDC center name

text: live

text: Generic

text: The reverse subtractive library of cDNA in testes of broodstock and juvenile shrimp

text: E. coli JM109

text: Testes

text: Penaeus monodon

text: Male

text: Shrimp Testis SSH Reverse Library

characteristic: title

text: Plasmid

text: pGEM-T Easy


text: Wuhan University

text: live

text: Generic

text: Adult

text: Scorpiops tibetanus

text: Venom gland

characteristic: tissue-type

text: Scorpiops tibetanus venom gland Library


text: ILVO

text: live

text: Generic

text: Flamenco

characteristic: cultivar

text: Rhododendron simsii

text: flower bud

text: Rhododendron simsii flower bud


text: ILVO

characteristic: INSDC first public

text: live

text: Generic

text: Rhododendron luteum

text: flower bud

text: Rhododendron luteum flower bud


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: Growth in Yeast Glucose (10g Yeast extract and 30 g glucose for 1 liter) at 25oC for 10 days The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 0.6-1.2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed at DOE Joint Genome Institute (Walnut Creek, CA).

text: Mycelium

text: Pseudocercospora fijiensis

text: SalI

text: NotI

text: whole

text: CBBT Mycosphaerella fijiensis MfEST3 (L)

characteristic: vector


text: DOE Joint Genome Institute

characteristic: INSDC last update

text: live

text: Generic

text: In Vivo Conditions: Growth in SNB minimal medium (1 g KH2PO4, 1 g KNO3, 0.5 g MgSO4.7H2O, 0.5 g KCl, 0.2 g glucose, 0.2 g sucrose for 1 liter) at 25oC for 10 days The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 1.2-2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed at DOE Joint Genome Institute (Walnut Creek, CA).

text: Mycelium

text: Pseudocercospora fijiensis

text: SalI

text: NotI

text: whole

text: CBBU Mycosphaerella fijiensis MfEST4 (M)


text: DOE Joint Genome Institute

text: live

characteristic: INSDC status

text: Generic

text: In Vivo Conditions: Growth in SNB minimal medium (1 g KH2PO4, 1 g KNO3, 0.5 g MgSO4.7H2O, 0.5 g KCl, 0.2 g glucose, 0.2 g sucrose for 1 liter) at 25oC for 10 days. The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 0.6-1.2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed at DOE Joint Genome Institute (Walnut Creek, CA).

text: Mycelium

text: Pseudocercospora fijiensis

text: SalI

text: NotI

text: whole

text: CBBW Mycosphaerella fijiensis MfEST4 (L)


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: Growth in SNB minimal medium without N source (1 g KH2PO4, 0.5 g MgSO4.7H2O, 0.5 g KCl, 0.2 g glucose, 0.2 g sucrose for 1 liter) at 25oC for 10 days. The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 0.6-1.2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed at DOE Joint Genome Institute (Walnut Creek, CA).

text: Mycelium

text: Pseudocercospora fijiensis

text: SalI

text: NotI

characteristic: strain

text: whole

text: CBBX Mycosphaerella fijiensis MfEST5 (L)


text: DOE Joint Genome Institute

text: live

text: Generic

characteristic: NCBI submission model

text: The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected >2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed at DOE Joint Genome Institute (Walnut Creek, CA).

text: DH10B T1 resistant

text: Pseudocercospora fijiensis

text: SalI

text: NotI

text: CBHT Mycosphaerella fijiensis MfEST3 Extract with glucose (H)


text: DOE Joint Genome Institute

text: live

text: Generic

characteristic: NCBI submission package

text: The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 0.6-1.2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed at DOE Joint Genome Institute (Walnut Creek, CA).

text: DH10B T1 resistant

text: Pseudocercospora fijiensis

text: SalI

text: NotI

text: CBHU Mycosphaerella fijiensis MfEST3 Extract with glucose (L)


text: DOE Joint Genome Institute

text: live

text: Generic

characteristic: description

text: DH10B T1 resistant

text: Pseudocercospora fijiensis

text: SalI

text: NotI

text: CBHW Mycosphaerella fijiensis MfEST4 Minimal medium with nitrogen (H)


text: DOE Joint Genome Institute

text: live

text: Generic

text: DH10B T1 resistant

text: Pseudocercospora fijiensis

text: SalI

text: NotI

text: CBHX Mycosphaerella fijiensis MfEST4 Minimal medium with nitrogen (L)


text: DOE Joint Genome Institute

text: live

text: Generic

text: DH10B T1 resistant

text: Pseudocercospora fijiensis

text: SalI

text: NotI

text: CBHY Mycosphaerella fijiensis MfEST5 Minimal medium without nitrogen (L)


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT)-primed cDNA and cloned using the SuperScript Plasmid System with Gateway Technology (Invitrogen Corp., Carlsbad, CA). Library constructed in the laboratory of M. Bronner at the California Institute of Technology (Pasadena, CA) and sequencing performed at the DOE Joint Genome Institute (Walnut Creek, CA).

text: Embryonic stages 2-12 days

characteristic: dev-stage

text: DH10B T1-resistant

text: Petromyzon marinus

text: SalI

text: NotI

text: CAAA Petromyzon marinus


text: University of Georgia

text: live

text: Generic

text: Larry Rosen, S1 and S2 population

text: The library was prepared from polyA+ RNA from premeiotic flower buds. Only floral buds with diameter of 1.5 mm or less were harvested for RNA isolation. Double-stranded cDNA was unidirectionally cloned into pBluescript SK- (Stratagene) with the 5' end of the cDNA inserted at the EcoRI site and the 3' polyA+ inserted at Xho1 site. A library-specific adaptor (GCGGCGTAG) was used to verify the library identity and then trimmed along with vector and low quality sequence. cDNA Library Statistics: Avg. insert length: 1715; Primary Titer: 2.7E6 pfu total; Amplified Titer: 2E10 pfu/ml; Antibiotic: 50 ug/ml Ampicillin. This library was generated by the Ancestral Angiosperm Project (www.AncestralAngiosperm.org) funded by NSF's Plant Genome Research Program (DBI-0638595).

text: premeiotic

text: SOLR (E. Coli)

characteristic: lab-host

text: Premeiotic flower buds

text: Aristolochia fimbriata

text: Flower buds

text: Aristolochia fimbriata flower bud library

text: Plasmid


text: University of Georgia

text: live

text: Generic

text: Larry Rosen, S1 and S2 population

text: The library was prepared from polyA+ RNA from a mix of roots, leaves, post-meiotic flower buds, flowers at anthesis, terminal buds and stem tissue, fruit, seedlings buds. Double-stranded cDNA was unidirectionally cloned into pBluescript SK- (Stratagene) with the 5' end of the cDNA inserted at the EcoRI site and the 3' polyA+ inserted at Xho1 site. A library-specific adaptor (GCCTGGTGG) was used to verify the library identity and then trimmed along with vector and low quality sequence. cDNA Library Statistics: Avg. insert length: 1389bp; Primary Titer: 3.0E6 pfu total; Amplified Titer: 2.3E10 pfu/ml; Antibiotic: 50 ug/ml Ampicillin. This library was generated by the Ancestral Angiosperm Project (www.AncestralAngiosperm.org) funded by NSF's Plant Genome Research Program (DBI-0638595).

text: SOLR (E. Coli)

text: roots, leaves, flower buds, flowers at anthesis, terminal buds and stem tissue, fruit, seedlings

text: Aristolochia fimbriata

characteristic: organism

text: Aristolochia fimbriata mixed tissue library

text: Plasmid


characteristic: External Id

text: University of British Columbia

text: live

text: Generic

text: Needles from 4 mature trees were harvested in the early fall (September). Equamolar amounts of mRNA from each treed was pooled for library construction. cDNA was prepared from 5 micrograms of mRNA and directionally ligated into the pBluescript II SK+ vector using Stratagenes pBluescript II XR cDNA Library Construction kit according to manufacturer's instructions, with modifications. Plasmid DNA was then transformed by electroporation into DH10B cells (Invitrogen). Clones used in sequencing were from the primary library.

text: E. coli DH10B

text: Picea sitchensis

text: EcoRI (5' end of the cDNA)

text: XhoI (3' end of the cDNA)

text: hermaphrodite

characteristic: sex

text: Needles

text: Plasmid

text: pBluescript II SK+