text: AgResearch Ltd.

text: live

characteristic: INSDC status

text: Generic

text: Bovine embryonic cDNA library derived from tissue harvested from an unknown breed calf by Rita Lee on 08/03/00

text: Bos taurus

text: Embryonic

text: BEMA

text: plasmid

text: pBK-CMV


status: PUBLIC

characteristic: External Id

text: AgResearch Ltd.

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Bovine lactating mammary gland cDNA library derived from tissue harvested from Jersey cow by Adrian Molenaar in January/February 1998

characteristic: description

text: Bos taurus

characteristic: organism

text: lactating mammary gland

tag: attribute

characteristic: tissue-type

text: BMGA

characteristic: title

text: plasmid

text: pBK-CMV

characteristic: vector


text: AgResearch Ltd.

text: live

text: Generic

text: Bovine Foetal Muscle cDNA library derived from tissue harvested from an unknown breed

text: Bos taurus

text: Foetal Muscle

text: BFMA

text: plasmid

text: pBK-CMV


text: AgResearch Ltd.

text: live

text: Generic

text: Bovine Small intestinal mucosal cDNA library derived from tissue harvested from a Friesian cross animal by Wayne Hein on 27/01/00

text: Bos taurus

text: Small intestine mucosa

text: BSIA

text: plasmid

text: pBK-CMV


text: AgResearch Ltd.

text: live

text: Generic

text: Bovine partial mammary subtracted cDNA library (VIAS library)

text: Bos taurus

text: partial mammary subtracted

text: BPMT

text: plasmid

text: pGEMTeasy


text: AgResearch Ltd.

text: live

text: Generic

text: Bovine ovary cDNA library 'A' derived from tissue harvested from an unknown breed calf on 28/09/99

text: Bos taurus

text: Ovarian

text: BOVA

text: plasmid

text: pBK-CMV


text: AgResearch Ltd.

text: live

text: Generic

text: Bovine mastitic mammary cDNA library

text: Bos taurus

text: mastitic mammary

text: BMAA

text: plasmid

text: pBK-CMV


text: AgResearch Ltd.

text: live

text: Generic

text: Bovine ipsilateral non-ovulated ovary cDNA library derived from tissue harvested from Friesian cows by Lora Hagemann on 07/12/99O

text: Bos taurus

text: Ovarian ipsilateral non ovulated

text: BINA

text: plasmid

text: pBK-CMV


text: AgResearch Ltd.

text: live

text: Generic

text: Bovine cardiac muscle cDNA library 'A' derived from tissue harvested from an unknown breed calf by Allan Crawford on 28/09/99

text: Bos taurus

text: Cardiac muscle

text: BCMA

text: plasmid

text: pBK-CMV


text: AgResearch Ltd.

text: live

text: Generic

text: Bovine mesenteric lymph node cDNA library derived from tissue harvested from a Friesian cross animal by Wayne Hein on 27/01/00

text: Bos taurus

text: Lymph node

text: BMNA

text: plasmid

text: pBK-CMV


text: AgResearch Ltd.

text: live

text: Generic

text: Bovine peyers patch cDNA library derived from tissue harvested from a Friesian cross animal by Wayne Hein on 27/01/00

text: Bos taurus

text: Peyers Patch

text: BPPA

text: plasmid

text: pBK-CMV


text: AgResearch Ltd.

text: live

text: Generic

text: Bovine anterior pituitary cDNA library derived from tissue supplied by Tom Wheeler on 01/01/00

text: Bos taurus

text: Anterior pituitary

text: BAPA

text: plasmid

text: pBK-CMV


text: AgResearch Ltd.

text: live

text: Generic

text: Bovine skeletal muscle cDNA library derived from tissue harvested from a unknown breed calf by Allan Crawford on 28/09/99

text: Bos taurus

text: Skeletal muscle

text: BSMA

text: plasmid

text: pBK-CMV


text: AgResearch Ltd.

text: live

text: Generic

text: Bovine heifer fat tissue cDNA library derived from tissue harvested from an Angus x Friesian heifer by Adrian Molenaar on 25/06/99

text: Bos taurus

text: Heifer Fat tissue

text: BHFA

text: plasmid

text: pBK-CMV


text: AgResearch Ltd.

text: live

text: Generic

text: Bovine tonsil cDNA library derived from tissue harvested from a Friesian cross animal by Wayne Hein on 27/01/00

text: Bos taurus

text: Tonsil

text: BTNA

text: plasmid

text: pBK-CMV


text: AgResearch Ltd.

text: live

text: Generic

text: Bovine mammary tissue - virgin cDNA library 'B' derived from tissue harvested from a Friesian cow by Adrian Molenaar on 01/01/00

text: Bos taurus

text: Virgin mammary

text: BMVB

text: plasmid

text: pBK-CMV


text: AgResearch Ltd.

text: live

text: Generic

text: Bovine hypothalamus cDNA library derived from tissue supplied by Tom Wheeler on 01/01/00

text: Bos taurus

text: Thyroid

text: BTYA

text: plasmid

text: pBK-CMV


text: AgResearch Ltd.

text: live

text: Generic

text: Bovine peripheral blood mononuclear cells cDNA library derived from cells harvested from two Friesian cross calves (vaccinated with BCG) by Bryce Buddle in April 2000

text: Bos taurus

text: peripheral blood mononuclear cells

text: BPMA

text: plasmid

text: pBK-CMV


text: AgResearch Ltd.

text: live

text: Generic

text: Bovine ovary cDNA library derived from tissue harvested from an unknown breed calf by Allan Crawford on 28/09/99

text: Bos taurus

text: Ovarian

text: BOVB

text: plasmid

text: pBK-CMV


text: AgResearch Ltd.

text: live

text: Generic

text: Bovine brain cortex cDNA library 'B' derived from tissue harvested from an unknown breed calf by Allan Crawford on 28/09/99

text: Bos taurus

text: Brain Cortex

text: BCXB

text: plasmid

text: pBK-CMV


text: AgResearch Ltd.

text: live

text: Generic

text: Life Tech Normalised library

text: Bos taurus

text: Life Tech Normalised library

text: BEMN

text: plasmid


text: AgResearch Ltd.

text: live

text: Generic

text: Bovine spleen stem cDNA library derived from tissue harvested from an unknown breed calf by Allan Crawford on 28/09/99

text: Bos taurus

text: Spleen

text: BSPB

text: plasmid

text: pBK-CMV


text: AgResearch Ltd.

text: live

text: Generic

text: Bovine Paratoid salivary cDNA library derived from tissue supplied by Tom Wheeler

text: Bos taurus

text: Paratoid Salivary

text: BPSA

text: plasmid

text: pBK-CMV


text: AgResearch Ltd.

text: live

text: Generic

text: Bovine lactating mammary gland cDNA library derived from tissue harvested from Jersey cow by Adrian Molenaar in January/February 1998 (Virtual casein subtracted from BMGA library)

text: Bos taurus

text: lactating mammary gland

text: BMGS

text: plasmid

text: pBK-CMV


text: AgResearch Ltd.

text: live

text: Generic

text: Bovine hypothalamus cDNA library derived from tissue supplied by Tom Wheeleron 01/01/00

text: Bos taurus

text: Hypothalamus

text: BHTA

text: plasmid

text: pBK-CMV


text: AgResearch Ltd.

text: live

text: Generic

text: Bovine mammary (late involution) cDNA library B derived from tissue harvested from an Angus cow by Adrian Molenaar on 01/01/00

text: Bos taurus

text: mammary (late involution)

text: BMLB

text: plasmid

text: pBK-CMV


text: AgResearch Ltd.

text: live

text: Generic

text: Bovine brain cerebellum cDNA library 'B' derived from tissue harvested from an unknown breed calf by Allan Crawford on 28/09/99

text: Bos taurus

text: Cerebellum Brain

text: BCEB

text: plasmid

text: pBK-CMV


text: AgResearch Ltd.

text: live

text: Generic

text: Bovine Thymus cDNA library

text: Bos taurus

text: thymus

text: BTMA

text: plasmid

text: pBK-CMV


text: AgResearch Ltd.

text: live

text: Generic

text: Bovine brain cerebellum cDNA library 'A' derived from tissue harvested from an unknown breed calf on 28/09/99

text: Bos taurus

text: Cerebellum Brain

text: BCEA

text: plasmid

text: pBK-CMV


text: AgResearch Ltd.

text: live

text: Generic

text: Bovine placental cDNA library derived from tissue harvested from an unknown breed cow by Rita Lee on 08/03/00

text: Bos taurus

text: Placenta

text: BPLA

text: plasmid

text: pBK-CMV


text: AgResearch Ltd.

text: live

text: Generic

text: Bovine posterior brain stem cDNA library derived from tissue harvested from an unknown breed calf by Allan Crawford on 28/09/99

text: Bos taurus

text: Posterior brain

text: BPBA

text: plasmid

text: pBK-CMV


text: AgResearch Ltd.

text: live

text: Generic

text: Bovine anterior brain stem cDNA library derived from tissue supplied by Allan Crawford on 28/09/99

text: Bos taurus

text: Anterior brain stem

text: BABA

text: plasmid

text: pBK-CMV


text: AgResearch Ltd.

text: live

text: Generic

text: Bovine liver cDNA library 'A' derived from tissue harvested from an unknown breed calf on 28/09/99

text: Bos taurus

text: Liver

text: BLIA

text: plasmid

text: pBK-CMV


text: AgResearch Ltd.

text: live

text: Generic

text: Bovine mammary tissue - virgin cDNA library 'A' derived from tissue harvested from a Friesian cow by Adrian Molenaar on 01/01/00

text: Bos taurus

text: Virgin mammary

text: BMVA

text: plasmid

text: pBK-CMV


text: AgResearch Ltd.

text: live

text: Generic

text: Bovine prescapular lymph node cDNA library derived from tissue harvested from a Friesian cross animal by Wayne Hein on 27/01/00

text: Bos taurus

text: Lymph node

text: BPNA

text: plasmid

text: pBK-CMV


text: AgResearch Ltd.

text: live

text: Generic

text: Bovine liver cDNA library 'B' derived from tissue harvested from an unknown breed calf by Allan Crawford on 28/09/99

text: Bos taurus

text: Liver

text: BLIB

text: plasmid

text: pBK-CMV


text: AgResearch Ltd.

text: live

text: Generic

text: Bovine Muscle cDNA library

text: Bos taurus

text: Muscle

text: BAMA

text: plasmid

text: pBK-CMV


text: AgResearch Ltd.

text: live

text: Generic

text: Combined BMAA and BMCA (both mastitic libraries )cDNA library

text: Bos taurus

text: mastitic mammary (BMAA combined)

text: BMUA

text: plasmid

text: pBK-CMV


text: AgResearch Ltd.

text: live

text: Generic

text: Bovine brain cortex cDNA library 'A' derived from tissue harvested from an unknown breed calf on 28/09/99

text: Bos taurus

text: Cortex

text: BCXA

text: plasmid

text: pBK-CMV


text: AgResearch Ltd.

text: live

text: Generic

text: Bovine 85 days pre-partum mammary tissue cDNA library derived from tissue harvested from an Angus cow by Adrian Molenaar on 01/01/00

text: Bos taurus

text: Pre-Partum mammary

text: BMPA

text: plasmid

text: pBK-CMV


text: AgResearch Ltd.

text: live

text: Generic

text: Bovine partial mammary subtracted cDNA library (VIAS library)

text: Bos taurus

text: partial mammary subtracted

text: BPMS

text: plasmid

text: pGEMTeasy


text: AgResearch Ltd.

text: live

text: Generic

text: Bovine contralateral - ovulated ovary cDNA library derived from tissue harvested from Friesian cows by Lora Hagemann on 07/12/99

text: Bos taurus

text: Ovarian ovulated

text: BCOA

text: plasmid

text: pBK-CMV


text: AgResearch Ltd.

text: live

text: Generic

text: Bovine ipsilateral ovulated ovary cDNA library derived from tissue harvested from Friesian cows by Lora Hagemann on 07/12/1999

text: Bos taurus

text: Ovarian ipsilateral ovulated

text: BIOA

text: plasmid

text: pBK-CMV


text: AgResearch Ltd.

text: live

text: Generic

text: Bovine spleen stem cDNA library 'A' derived from tissue harvested from an unknown breed calf on 28/09/99

text: Bos taurus

text: Spleen

text: BSPA

text: plasmid

text: pBK-CMV


text: AgResearch Ltd.

text: live

text: Generic

text: Bovine Testes cDNA library derived from tissue supplied by Rita Lee on 09/11/98

text: Bos taurus

text: Testes

text: BTSA

text: plasmid

text: pBK-CMV


text: AgResearch Ltd.

text: live

text: Generic

text: Bovine Seminal vesicle cDNA library derived from tissue supplied by Rita Lee on 09/11/98

text: Bos taurus

text: Seminal Vesicle

text: BSVA

text: plasmid

text: pBK-CMV


text: AgResearch Ltd.

text: live

text: Generic

text: Bovine placental cDNA library derived from tissue harvested from an unknown breed cow

text: Bos taurus

text: Placenta

text: BPLC

text: plasmid

text: pBK-CMV


text: AgResearch Ltd.

text: live

text: Generic

text: Bovine contralateral, non-ovulated ovary cDNA library derived from tissue harvested from Friesian cows by Lora Hagemann on 07/12/99

text: Bos taurus

text: Ovarian non ovulated

text: BCNA

text: plasmid

text: pBK-CMV


text: AgResearch Ltd.

text: live

text: Generic

text: Bovine adrenal gland cDNA library derived from tissue supplied by Tom Wheeler on 01/01/00

text: Bos taurus

text: Adrenal gland

text: BAGA

text: plasmid

text: pBK-CMV


text: Zhongshan University

text: live

text: Generic

text: Zea mays

text: Zea mays library (Dai Q)


text: Washington University

text: live

text: Generic

text: BD Biosciences 5' RACE kit primed with gene specific primer and 5'CDS, SMARTII A oligo and UPM primers

text: 3 weeks

characteristic: dev-stage

text: Arabidopsis thaliana

characteristic: strain

text: mixed leaves

text: Arabidopsis thaliana 5' RACE


text: Washington University

text: live

text: Generic

text: BD Biosciences 3' RACE kit primed with gene specific primer and 3'CDS primer A and UPM primers

text: 3 weeks

text: Arabidopsis thaliana

text: Col ddm1-1

text: mixed leaves

text: Arabidopsis thaliana 3' RACE


text: School of Medicine of Xian Jiaotong University

text: live

text: Generic

text: bladder transitional cell

characteristic: cell type

characteristic: cell-line

text: A differentially expressed gene subtractive library was constructed by using subtractive suppression hybridization between the pair of bladder transitional cell lines BLZ-211 and BLS-211 with different metastatic potentials. These EST fragments were ligated with pGEM-T vectors and amplified in JM109 E. coli. After blue-white screening, the white positive inserts were picked out and stored.

text: adult

text: bladder

characteristic: organ

text: Homo sapiens

text: male

characteristic: sex

text: Homo sapiens bladder transitional cell carcinoma cell lines subtractive library

text: Plasmid

text: pGEM-T


characteristic: External Id

text: Woods Hole Oceanographic Institution

text: live

characteristic: INSDC status

text: Generic

text: N- or P-limited

text: 3'RACE products generated using MPSS signatures

text: Alexandrium fundyense

text: 3'RACE amplification products


text: National Cancer Institute / NIH

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: cDNA was primed using oligo-dT primer: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3' and cloned into the EcoRV/NotI sites of pExpress-1. Size-selection >1.3kb resulted in an average insert size of 2.0kb. This is a non-normalized primary library (normalized primary library is NIH_MGC_366) and was constructed by Express Genomics (Frederick, MD) for the Mammalian Gene Collection http://mgc.nci.nih.gov/ .

text: DH10B TonA

characteristic: lab-host

text: brain/CNS

text: Rattus norvegicus

text: EcoRV

text: NotI

text: whole brain, pool of 7

text: plasmid


text: National Cancer Institute / NIH

characteristic: INSDC last update

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: cDNA was primed using oligo-dT primer: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3' and cloned into the EcoRV/NotI sites of pExpress-1. Size-selection >1.3kb resulted in an average insert size of 1.8kb. This is a normalized primary library to Cot5 (non-normalized primary library is NIH_MGC_365) and was constructed by Express Genomics (Frederick, MD) for the Mammalian Gene Collection http://mgc.nci.nih.gov/ .

text: DH10B TonA

text: brain/CNS

text: Rattus norvegicus

text: EcoRV

text: NotI

text: whole brain, pool of 7

text: plasmid


text: National Cancer Institute / NIH

text: live

text: Generic

text: cDNA was primed using oligo-dT primer: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3' and cloned into the EcoRV/NotI sites of pExpress-1. Size-selection >1.3kb resulted in an average insert size of 1.8kb. This is a non-normalized primary library (normalized primary library is NIH_MGC_368) and was constructed by Express Genomics (Frederick, MD) for the Mammalian Gene Collection http://mgc.nci.nih.gov/ .

characteristic: description

text: DH10B TonA

text: liver

text: Rattus norvegicus

characteristic: organism

text: EcoRV

text: NotI

text: whole liver, pool of 7

characteristic: tissue-type

text: plasmid


text: Universidad Nacional de General San Martin

text: live

text: Generic

text: Tinf_4it: Triatoma infestans fourth instar cDNA library, in pDNRlib. Total RNA was isolated from Triatoma infestans IV instar specimens with TRIzol reagent (Invitrogen). Residual genomic DNA was removed by DNase treatment using RNase-free DNase I (Ambion). Poly(A)+ RNA was purified using the PolyATtract mRNA Isolation System IV (Promega). cDNA libray was constructed with SMART cDNA Synthesis Kit (Clontech). All steps were performed according to the manufacturer?s instructions.

text: 4th instar

characteristic: dev-stage

text: Triatoma infestans

text: whole body

characteristic: title

text: plasmid

text: pDNR-LIB

characteristic: vector


text: live

text: Generic

text: Tinf_4itc: Triatoma infestans fourth instar cDNA library. Total RNA was isolated from Triatoma infestans IV instar specimens with TRIzol reagent (Invitrogen). Residual genomic DNA was removed by DNase treatment using RNase-free DNase I (Ambion). Poly(A)+ RNA was purified using the PolyATtract mRNA Isolation System IV (Promega). All steps were performed according to the manufacturer?s instructions. cDNA library was constructed using the PCR-Select cDNA Subtraction Kit (Clontech) as unsubtracted libraries, which implies ligating the sample with both adaptors at the same time, and not performing the hybridization steps.

text: 4th instar

text: Triatoma infestans

text: whole body

text: plasmid

text: pGEM-T Easy


text: live

text: Generic

text: Tinf_4itla: Triatoma infestans fourth instar cDNA library. Total RNA was isolated from Triatoma infestans IV instar specimens with TRIzol reagent (Invitrogen). Residual genomic DNA was removed by DNase treatment using RNase-free DNase I (Ambion). Poly(A)+ RNA was purified using the PolyATtract mRNA Isolation System IV (Promega). All steps were performed according to the manufacturer?s instructions. cDNA library was constructed using the PCR-Select cDNA Subtraction Kit (Clontech) as unsubtracted libraries, which implies ligating the sample with both adaptors at the same time, and not performing the hybridization steps.

text: 4th instar

text: Triatoma infestans

text: whole body

text: plasmid

text: pGEM-T Easy


text: live

text: Generic

text: Tinf_4itn: Triatoma infestans fourth instar cDNA library. Total RNA was isolated from Triatoma infestans IV instar specimens with TRIzol reagent (Invitrogen). Residual genomic DNA was removed by DNase treatment using RNase-free DNase I (Ambion). Poly(A)+ RNA was purified using the PolyATtract mRNA Isolation System IV (Promega). All steps were performed according to the manufacturer?s instructions. cDNA library was constructed using the PCR-Select cDNA Subtraction Kit (Clontech) as unsubtracted libraries, which implies ligating the sample with both adaptors at the same time, and not performing the hybridization steps.

text: 4th instar

text: Triatoma infestans

text: whole body

text: plasmid

text: pGEM-T Easy


text: live

text: Generic

text: Tinf_4itnv: Triatoma infestans fourth instar cDNA library. Total RNA was isolated from Triatoma infestans IV instar specimens with TRIzol reagent (Invitrogen). Residual genomic DNA was removed by DNase treatment using RNase-free DNase I (Ambion). Poly(A)+ RNA was purified using the PolyATtract mRNA Isolation System IV (Promega). All steps were performed according to the manufacturer?s instructions. cDNA library was constructed using the PCR-Select cDNA Subtraction Kit (Clontech) as unsubtracted libraries, which implies ligating the sample with both adaptors at the same time, and not performing the hybridization steps.

text: 4th instar

text: Triatoma infestans

text: whole body

text: plasmid

text: pGEM-T Easy


text: live

text: Generic

text: Tinf_4itORE: Triatoma infestans fourth instar ORESTES library. Total RNA was isolated from Triatoma infestans IV instar specimens with TRIzol reagent (Invitrogen). Residual genomic DNA was removed by DNase treatment using RNase-free DNase I (Ambion). Poly(A)+ RNA was purified using MicroPoly(A)Purist Kit (Ambion). All steps were performed according to the manufacturer?s instructions. ORESTES libraries were constructed as described by Diaz-Neto E. et al. (1999) with minor modifications.

text: 4th instar

text: Triatoma infestans

text: whole body

text: plasmid

text: pGEM-T Easy


text: live

text: Generic

text: Tinf_4its: Triatoma infestans fourth instar ORESTES library. Total RNA was isolated from Triatoma infestans IV instar specimens with TRIzol reagent (Invitrogen). Residual genomic DNA was removed by DNase treatment using RNase-free DNase I (Ambion). Poly(A)+ RNA was purified using the PolyATtract® mRNA Isolation System IV (Promega). All steps were performed according to the manufacturer?s instructions. ORESTES libraries were constructed as described by Diaz-Neto E. et al. (1999) with minor modifications.

text: 4th instar

text: Triatoma infestans

text: whole body

text: plasmid

text: pGEM-T Easy


text: live

text: Generic

text: Tinf_aii: Triatoma infestans adult gut cDNA library. Total RNA was isolated from Triatoma infestans adult gut samples with TRIzol reagent (Invitrogen). Residual genomic DNA was removed by DNase treatment using RNase-free DNase I (Ambion). Poly(A)+ RNA was purified using the PolyATtract mRNA Isolation System IV (Promega). All steps were performed according to the manufacturer?s instructions. cDNA library was constructed using the PCR-Select cDNA Subtraction Kit (Clontech) as unsubtracted libraries, which implies ligating the sample with both adaptors at the same time, and not performing the hybridization steps.

text: adult

text: Triatoma infestans

text: gut

text: plasmid

text: pGEM-T Easy


text: live

text: Generic

text: Tinf_am: Triatoma infestans adult muscle ORESTES library. Total RNA was isolated from Triatoma infestans adult muscle samples with TRIzol reagent (Invitrogen). Residual genomic DNA was removed by DNase treatment using RNase-free DNase I (Ambion). Poly(A)+ RNA was purified using MicroPoly(A)Purist Kit (Ambion). All steps were performed according to the manufacturer?s instructions. ORESTES libraries were constructed as described by Diaz-Neto E. et al. (1999) with minor modifications.

text: adult

text: Triatoma infestans

text: muscle

text: plasmid

text: pGEM-T Easy


text: Graduate University of China Academy of Sciences

text: live

text: Generic

text: RNA isolated from leaves of cadmium stressed Phytolacca americana Linn. and the SSH library was constructed using PGEM-T vector.

text: seedling

text: Phytolacca americana

text: leaf

text: Cadmium-stressed Phytolacca americana SSH Library


text: Guangxi University

text: live

text: Generic

characteristic: cultivar

text: Siraitia grosvenorii

text: leaf and fruit

text: Luohanguo leaf Library


text: University of Pretoria

text: live

text: Generic

text: The first two leaves of three week old pearl millet breeding line 842B seedlings were harvested and immediately frozen in liquid nitrogen. RNA isolation was performed using the RNAeasy Minikit (Qiagen) according to the manufacturer's protocol. DNA was removed by digestion with 1 unit DNaseI (Fermentas, Burlington, Canada) and purified using an RNeasy Minelute cleanup kit (Qiagen, Hilden, Germany). The pure RNA was used to synthesize first strand cDNA using 200 units of Superscript III Reverse Transcriptase (Invitrogen, California, USA); 500 ng oligo(dT)12-18 (Invitrogen), 1 ug RNA, dNTPs at a final concentration of 0.5mM, 0.25uM DDT, 40 units RNaseOUT in a 20 ul reaction according to manufacturer's protocol. Forward primer 2 (F2) (5' AGCTGCTTGGAAGGATGAAG 3') was combined with Reverse primer 2 (R2) (5' TTTGGGTCATAGTATTCCTTGC 3') was used to amplify a fragment of the expressed pearl millet PDS gene from cDNA. The pearl millet PDS amplicon was ligated into p-GEM T Easy (Promega, Madison, USA) vector system

text: Three week old seedlings

text: Cenchrus americanus

text: Leaf material

text: Pennisetum glaucum cv. 842B leaf cDNA library

text: p-GEM T Easy


text: Institute of Himalayan Bioresource Technology

text: live

text: Generic

text: Apical bud

characteristic: organ

text: Caragana jubata

text: Apical bud

text: Caragana jubata differentially expressed ESTs in response to low-temperature.

text: plasmid

text: PCR-TRAP (GenHunter)


text: Kunming University of Science and Technology

text: live

text: Generic

text: Yunnan Small Black Soybean

text: exposed to Al solution for 30min,1h,2h,4h,8h,12h,24h respectively

text: crop

text: Glycine max

text: root

text: Root Supression Substractive Hybridization cDNA Library of Yunnan Small Black Soybean

text: pGEM-T Easy Vector


text: National Institute of Agricultural Science and Technology, RDA

text: live

text: Generic

text: E.coli (10X6) treatment 24 hours after inoculation in Lumbricus terrestris.

text: Lumbricus terrestris

text: hermaphrodite

characteristic: sex

text: whole body

text: Lumbricus terrestris antibacterial related cDNA library


characteristic: External Id

text: Agricultural Genetic Engineering Research Institute (AGERI),

text: live

text: Generic

text: Egypt

characteristic: country

text: 6 weeks old

text: Vicia monantha

text: Vicia monantha 6 weeks old


text: Genoscope - Centre National de Sequencage

characteristic: INSDC center name

text: live

characteristic: INSDC status

text: Generic

text: Oikopleura dioica

text: unfertilized eggs (KT0ABA)

text: pBluescript II KS(+)


characteristic: INSDC first public

text: live

text: Generic

text: Oikopleura dioica

text: embryos (KT0ACA)

text: pBluescript II KS(+)


characteristic: INSDC last update

text: live

text: Generic

text: Oikopleura dioica

text: mixed stages (KT0AAA)

text: pBluescript II KS(+)


text: University of Wurzburg

text: live

text: Generic

characteristic: NCBI submission model

text: subtracted cDNA library

text: adult (major workers)

text: Camponotus floridanus

text: colony C90

characteristic: strain

text: midgut and fat body from immune challenged animals

text: Camponotus floridanus subtracted cDNA library


text: Dong-A University

text: live

text: Generic

characteristic: NCBI submission package

text: Stratagene (cat. #200450); Total RNA was extracted separately from leaf tissues, using the method described by Chung et al. (2009). mRNA was reverse transcribed into double stranded cDNA using a modified oligo (dT)18 primer with an identifying tag sequence. Double stranded cDNAs were size selected (more then 500 bp), adaptored with EcoRI adapters at both ends and then digested with XhoI. The cDNAs were then directionally cloned into EcoRI-XhoI digested pBSII SK(+) phagemid vector (Stratagene). The total number of white colony forming units (cfu) was 0.8x100,000,000 cfu. The background of empty clones was less than 10%. Inserts ranged from 0.3 kb to 5kb, as determined by PCR.

characteristic: description

text: mature

text: leaf

text: Suaeda glauca

text: salt stress treated Suaeda glauca leaf library


text: College of Life Sciences, Zhejiang University

text: live

text: Generic

text: Subtractive hybridization was done using total RNA from hepatopancreas of G. yunohana as tester and total RNA from hepatopancreas of Portunus trituberculatus as driver. The products were cloned into PUC19 vector at Sal I site, and transformed into E. coli DH5¦Á (Invitrogen, USA).

text: Gandalfus yunohana

characteristic: organism

text: Sal I

text: Gandalfus yunohana hepatopancreas library

text: Plasmid


text: INRA - UMR BIOGECO

text: live

text: Generic

text: one year old cuttings

characteristic: dev-stage

text: Quercus robur

text: white root

characteristic: tissue-type

text: Quercus robur cDNA-AFLP


text: National Research Institute of Aquaculture, Fisheries Research Agency

text: live

text: Generic

text: cDNA library of monoclonal antibody FE1 positive Japanese flounder leucocyte subpopulation was constructed using Creator SMART cDNA library Construction Kit (Clountech, United States) following manufacturer's protocol. First strand cDNA was synthesized from a total of 50 ng of total RNA then subjected LD PCR. The LD PCR products were then digested by Sfi I to ligate into pDNR-LIB vector. The ligation products were electoroporeted into competent Escherichia coli Top10. Single colonies were picked for the cDNA sequencing.

text: adult

text: Paralichthys olivaceus

text: mix of male and female

text: monoclonal antibody FE1 positive leucocyte

text: FE-1 positive leukocytes cDNA library

characteristic: title


text: National Research Institute of Aquaculture

text: live

text: Generic

text: The peripheral blood leukocyte (PBL) was separated from the fish that had the history of infection and naive fish. PBL was incubated with viral hemorrhagic septicemia virus (VHSV) and harvested at 4, 8, 24 hours after incubation. mRNA was extracted from PBL and pooled with each experimental group. The pooled mRNA from PBL of the fish that had the history of infection and naive fish was used to make tester and driver cDNAs, respectively. Two subtractions were performed: the original intended subtraction (forward subtraction, clone name: VV-CV), and a reverse subtraction (clone name: CV-VV) in which tester serves as the driver and the driver as tester. Subtractive hybridization was performed with the PCR-Select cDNA Subtraction Kit (Clontech), according to the manufacture's protocol.

text: adult

text: Paralichthys olivaceus

text: peripheral blood leucocyte

text: subtracted flounder cDNA library


text: School of Bioscience and Biotechnology, Faculty of Science and Technology, National University of Malaysia

text: live

text: Generic

text: Salicylic acid elicitation was carried out by treating approximately 2 month-old Polygonum minus in vitro plantlets with 0.5mM of Salicylic acid for 3 days whereas the untreated plantlets were served as negative control. Total RNA from treated and control Polygonum minus leaves were extracted using the modified Lopez-Gomez and Gomez Lim (1992) method. The mRNA was then isolated from the total RNA using PolyATtract mRNA Isolation Kit. A subtractive cDNA library was constructed by using the PCR-Select cDNA subtraction kit from Clontech, USA. The treated leaves were served as cDNA tester while the untreated leaves were served as cDNA driver in the subtractive cDNA library preparation. 2 rounds of hybridization steps were performed to enrich the differentially expressed mRNA transcripts and the transcripts were further amplified by primary PCR and nested PCR. The secondary PCR products were then cloned into pGEM-T Easy vector and transformed into E. coli TOP10. Blue-white screening was performed and white colonies were randomly selected to be analysed by PCR and reverse Northern analysis. All clones with strong hybridization signals representing salicylic acid responsive genes were sent for sequencing. Potential clones responsible for aromatic compounds production and plant defence system as well as abiotic stress were selected to be analysed by RT-PCR.

text: E. coli strain TOP 10

characteristic: lab-host

text: Leaves

text: Persicaria minor

text: Salicylic acid-treated x non-treated Polygonum minus leaves subtractive library

text: pGEM-T Easy Vector

characteristic: vector


text: Mubarak City for Scientific Research and Technology Applications

text: live

text: Generic

text: AUC 95

characteristic: cultivar

text: Solanum lycopersicum

text: Lycopersicon esculentum cv. AUC 95 BAC library


characteristic: External Id

text: University Of Calcutta

text: live

text: Generic

text: JRC 412

text: Leaf samples of one month old JRC 412 ( a susceptible accession of jute) against Macrophomina phaseolina infection) were either inoculated with the mycelia culture of Macrophomina phaseolina, or were imbibed in BABA solution (20ug/ml) for 24 hours, or were BABA primed (for 24 hours) and then inoculated with the fungal culture. After 12hr post infection, total RNA were isolated from the respective samples by using TRI Reagent(Ambion). The differentially expressed transcripts induced upon infection and BABA priming were identified by Differential Display analysis using RNA Image kit (GenHunter). The differentially expressed cDNAs were cloned into PCR-TRAP cloning system (GenHunter Corporation) and transformed into Gh-competent cells (GenHunter Corporation). Sequencing was performed in an ABI Prism 3100 Genetic Analyzer (Applied Biosystem).

text: Corchorus capsularis

text: Leaf tissue

text: Library of differentially expressed transcripts in jute upon stress


text: University Federal of Sao Carlos

text: live

text: Generic

text: Penaeus vannamei

text: Shrimp larval library


text: INRA

text: live

text: Generic

text: cell cultures

characteristic: cell type

text: Montfavet 63-5

text: EST obtained by RaSH between two interactions tomato cells/Fusarium oxysporum: (i) tomato cells inoculated with germinated conidia of protective F. oxysporum (Fom24), (ii) tomato cells inoculated with germinated conidia of non-protective mutant F. oxysporum (rev157)

text: Solanum lycopersicum

text: XhoI

text: XhoI

text: tomato cells/non protective F. oxysporum (rev157) interaction (Solanum lycopersicum)

text: Plasmid

text: pBluescript SK


text: INRA

characteristic: INSDC center name

text: live

text: Generic

text: Fusarium oxysporum f. sp. melonis

text: XhoI

text: XhoI

text: protective F. oxysporum (Fom24)/tomato cells interaction (Fusarium oxysporum f. sp. melonis)

text: Plasmid

text: pBluescript SK


text: INRA

text: live

characteristic: INSDC status

text: Generic

text: EST obtained by RaSH between two interactions tomato cells/Fusarium oxysporum: (i) tomato cells inoculated with germinated conidia of protective F. oxysporum (Fopm24), (ii) tomato cells inoculated with germinated conidia of non-protective mutant F. oxysporum (rev157)

text: Solanum lycopersicum/Fusarium oxysporum mixed EST library

text: XhoI

text: XhoI

text: tomato cells/protective F. oxysporum (Fom24) interaction (undetermined source)

text: Plasmid

text: pBluescript SK


text: INRA

characteristic: INSDC first public

text: live

text: Generic

text: XhoI

text: XhoI

text: tomato cells/non protective F. oxysporum (rev157) interaction (undetermined source)

text: Plasmid

text: pBluescript SK


text: INRA

characteristic: INSDC last update

text: live

text: Generic

text: Fusarium oxysporum

text: XhoI

text: XhoI

text: non protective F. oxysporum (rev157)/tomato cells interaction (Fusarium oxysporum)

text: Plasmid

text: pBluescript SK


text: INRA

text: live

text: Generic

characteristic: NCBI submission model

text: cell cultures

text: Montfavet 63-5

text: EST obtained by RaSH between two interactions tomato cells/Fusarium oxysporum: (i) tomato cells inoculated with germinated conidia of protective F. oxysporum (Fom24), (ii) tomato cells inoculated with germinated conidia of non-protective muant F. oxysporum (rev157)

text: Solanum lycopersicum

text: XhoI

text: XhoI

text: tomato cells/protective F. oxysporum (Fom24) interaction (Solanum lycopersicum)

text: Plasmid

text: pBluescript SK


text: Academia Sinica

text: live

text: Generic

text: Oncidium hybrid cultivar

text: Oncidium 'Gower Ramsey' flower cDNA Library


text: National Institute of Allergy and Infectious Diseases

text: live

text: Generic

text: Unidirectional library was constructed using long distance PCR (Smart cDNA kit) and Lambda TriplEx2 vector

text: adult

text: salivary glands

characteristic: organ

text: Nyssomyia umbratilis

text: female

characteristic: sex

text: Adult female salivary gland cDNA library LumbSG1

text: Phage

text: Lambda TriplEx2


text: live

text: Generic

characteristic: NCBI submission package

characteristic: description

text: adult

text: salivary glands

text: Simulium guianense

text: female

text: Adult female salivary gland cDNA library Sguia-sg-1

text: Phage

text: Lambda TriplEx2


text: The First Institute of Oceanography, State Oceanic Administration

text: live

text: Generic

text: Employing ZAP-cDNA Synthesis Kit (Stratagene, La Jolla, CA, USA) and ZAP-cDNA Gigapack III Gold Cloning Kit (Stratagene) for library construction.

text: Kappaphycus alvarezii

characteristic: organism

text: Kappaphycus alvarezii cDNA library


text: Ruhr-Universitat Bochum

text: live

text: Generic

text: Pyronema omphalodes

characteristic: strain

text: Pyronema omphalodes SSH


text: Bio-Protection Research Centre

text: live

text: Generic

text: Spongospora subterranea

text: Solanum tuberosum Spongospora subterranea callus

characteristic: title


text: Bio-Protection Research Centre

text: live

text: Generic

text: Plasmodiophora brassicae

text: Brassica rapa Plasmodiophora brassicae callus


text: Wellcome Trust Sanger Institute

text: live

text: Generic

text: Glossina palpalis

text: head

characteristic: tissue-type

text: Glossina palpalis head


text: Wellcome Trust Sanger Institute

text: live

text: Generic

text: Glossina tachinoides

text: head

text: Glossina tachinoides head


text: Wellcome Trust Sanger Institute

text: live

text: Generic

text: Glossina pallidipes

text: antenna

text: Glossina pallidipes antenna


text: Huazhong Agricultural University

text: live

text: Generic

text: nymphs of third and fourth instar

characteristic: dev-stage

text: rice varieties of TN1

characteristic: lab-host

text: Nilaparvata lugens

text: Nilaparvata lugens illumina library


characteristic: External Id

text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: mycelium grown in liquid YMPG medium The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 0.6-3kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed and sequencing performed at the DOE Joint Genome Institute (Walnut Creek, CA).

text: Phanerochaete carnosa

text: SalI

text: NotI

text: mycelium

text: CCUT Phanerochaete carnosa HHB-10118-sp mycelium grown in liquid YMPG medium

characteristic: vector


text: DOE Joint Genome Institute

characteristic: INSDC center name

text: live

text: Generic

text: In Vivo Conditions: mycelium grown in liquid YMPG medium The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected >3kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed and sequencing performed at the DOE Joint Genome Institute (Walnut Creek, CA).

text: Phanerochaete carnosa

text: SalI

text: NotI

text: mycelium

text: CFBN Phanerochaete carnosa HHB-10118-sp mycelium grown in liquid YMPG medium


text: Institute of Terrestrial Ecology

text: live

characteristic: INSDC status

text: Generic

text: Irvingia gabonensis

text: Irvingia gabonensis DNA


text: Seoul National University

characteristic: INSDC first public

text: live

text: Generic

text: Homo sapiens

text: Homo sapiens DNA (Kim S)


text: Centre Rene Huguenin

characteristic: INSDC last update

text: live

text: Generic

text: lymphocytes

characteristic: cell type

text: PAC clones in E. coli DH10B. For PAC library availability, please contact Pieter de Jong (BACPACorders@mail.cho.org). Clones may be purchased from BACPAC Resources (www.chori.org/bacpac) or from Research Genetics (info@resgen.com)

text: E. coli DH10B

text: Homo sapiens

text: female


text: Children's Hospital Oakland Research Institute

text: live

text: Generic

characteristic: NCBI submission model

text: Spleen

text: PAC clones in E. coli DH10B

text: Mus musculus

text: Female

text: RPCI-21 Mouse (129S6/SvEvTac) genomic library


text: live

text: Generic

text: Spleen

text: BAC clones in E. coli DH10B

text: Mus musculus

text: Female

characteristic: sex

text: RPCI-22 Mouse (129S6/SvEvTac) genomic library


text: live

text: Generic

characteristic: NCBI submission package

text: Kidney and brain

text: BAC clones in E. coli DH10B

text: Mus musculus

text: Female

text: RPCI-23 Mouse (C57BL/6J) genomic library


text: Medical and Molecular Genetics Centre-IRO

text: live

text: Generic

text: Homo sapiens


text: live

text: Generic

text: Homo sapiens

text: Research Genetics CITB B&C


text: Pearl River Fisheries Research Institute

text: live

text: Generic

text: Siniperca chuatsi Virus is a unclassed Virus. Its genome is dsDNA, about 20Kbp. The nucleic acid was isolated from the fish (Siniperea chuatsi) infected with Siniperea chuatsi virus (SCV) and purified from the low melting-temperature agarose. Random primer (contain the enzyme position) were used to amplify SCV genome. The fragments of PCR productions were isolated and cloned in pUC19.

characteristic: description

text: Siniperca chuatsi

text: Siniperca chuatsi virus

text: A RAPD Segment Sequence of Siniperca chuatsi Virus


text: Cordoba University

text: live

text: Generic

text: Picual

characteristic: cultivar

text: Olea europaea

characteristic: organism

text: Olea europaea Picual RAPD


text: Cordoba University

text: live

text: Generic

text: Hojiblanca

text: Olea europaea

text: Olea europaea Hojiblanca RAPD

characteristic: title


text: University of Pennsylvania School of Medicine

text: live

text: Generic

characteristic: cell-line

text: The mouse ES library was created by ligating large-size DNA fragments from RW4 cells into the HindIII cloning site of the pBeloBAC11 vector. The recombinant vector was then electroporated into DH10B host cells. The inserts for the library BAC clone are ~120kb and are flanked in the vector by T7 and Sp6 RNA promoters on either side of the HindIII cloning site. The library was constructed by Genome Systems (now Incyte).

text: E.coli

text: Mus musculus

text: HindIII

text: HindIII

characteristic: strain

text: Mouse ES genomic library

text: BAC


text: University Childrens Hospital

text: live

text: Generic

text: Homo sapiens

text: Human PAC clone (F.Hildebrandt)


text: Clark Atlanta University

text: live

text: Generic

text: Homo sapiens

text: Human mega YAC 2001C6


text: University of Malta

text: live

text: Generic

text: Capra hircus

text: Maltese goat (Capra hircus hircus) library


text: Ruhr University Bochum

text: live

text: Generic

text: Homo sapiens

text: Homo sapiens library (Traeger T)


text: University of Calgary

text: live

text: Generic

text: Homo sapiens

text: CITB-978K-B, CITB-HSP-C


text: BIOS Laboratories

text: live

text: Generic

text: lymphoblastoid

text: Homo sapiens

text: Homo sapiens GMO3714


text: University of Tasmania

text: live

text: Generic

text: Euphausia superba

text: Euphausia superba library (Jarman SN)


text: INRA

text: live

text: Generic

text: Capra hircus

text: Saanen

text: Capra hircus Saanen


text: Colorado State University

text: live

text: Generic

text: Mycobacterium leprae

text: ordered cosmid library


text: Roswell Park Cancer Institute

text: live

text: Generic

text: Mus musculus

text: Mus musculus 129/SvEvTACfBr


text: Moredun Research Institute

text: live

text: Generic

text: biotype: F38; aetiological agent of contagious caprine pleuropneumonia (CCPP)

text: Mycoplasma capricolum subsp. capripneumoniae

text: Mycoplasma capricolum capripneumoniae biotype F38


text: NIMH-NIH: Bldg.10:2D56

text: live

text: Generic

text: BAC insert generated by partial HindIII digest of human genomic DNA

text: Homo sapiens

text: Homo sapiens BAC (Zullo SJ)


text: NIMH-NIH: Bldg.10:2D56

text: live

text: Generic

text: BAC insert generated by partial HindIII digest of mouse genomic DNA

text: Mus musculus

text: Mus musculus BAC (Zullo SJ)


text: NIMH-NIH: Bldg.10:2D56

text: live

text: Generic

text: Research Genetics Library CIT-B-978SK-B Cat# 96012. (Phone:800-533-4363)

text: Homo sapiens

text: BAC Library CITB-978SK-B, Cat. #96012


text: University of South Florida

text: live

text: Generic

text: Riftia pachyptila

text: Riftia pachyptila anonymous nuclear DNA


text: University of South Florida

text: live

text: Generic

text: Chelonia mydas

text: Chelonia mydas anonymous nuclear DNA


text: Washington Univ. Sch. of Medicine

text: live

text: Generic

text: Homo sapiens Epstein-Barr virus transformed B-lymphoblastoid cell line derived from a normal male Caucasian donor

text: Homo sapiens


text: Scripps Institution of Oceanography, UCSD

text: live

text: Generic

text: manganese oxidizing bacterium

text: Pseudomonas putida

text: MnB1; ATCC 23483

text: Pseudomonas putida MnB1; ATCC 23483


text: c/o CREATOGEN GmbH

text: live

text: Generic

text: Salmonella enterica subsp. enterica serovar Typhimurium

text: ATCC 14028

text: Salmonella typhimurium ATCC14028


text: Institute for Zoo Biology and Wildlife Research

text: live

text: Generic

text: Falco peregrinus

text: male

text: Brandenburg (Germany)

text: Falco peregrinus Brandenburg (Germany) male


text: live

text: Generic

text: Falco peregrinus

text: female

text: Brandenburg (Germany)

text: Falco peregrinus Brandenburg (Germany) female


text: National Cancer Institute, FCRDC

text: live

text: Generic

text: Felis catus

text: Felis catus DNA (Lyons LA)


text: National Cancer Institute, FCRDC

text: live

text: Generic

text: Felis catus

text: muscle

characteristic: tissue-type

text: Felis catus muscle


characteristic: External Id

text: Oklahoma State University

text: live

text: Generic

text: PCR amplified sequences resulting from the genomic subtraction of Listeria monocytogenes and Listeria innocua/Listeria ivanovii genomic DNA

text: Listeria monocytogenes

text: Scott A

text: Listeria monocytogenes Scott A


text: INRA

text: live

text: Generic

text: Bos taurus

text: Bos taurus DNA (Slimane W)


text: INRA

text: live

text: Generic

text: adult

characteristic: dev-stage

text: Bos taurus

text: blood

text: Bos taurus blood adult


text: University of Washington, The V.M. Bloedel Hearing Research Center

characteristic: INSDC center name

text: live

text: Generic

text: Alu digest

text: Mus musculus

text: Mus musculus C57BL/6J YAC TRP1


text: live

characteristic: INSDC status

text: Generic

text: Alu digest

text: Mus musculus

text: Spretus

text: Mus musculus Spretus YAC TRP1


characteristic: INSDC first public

text: live

text: Generic

text: Alu digest

text: Mus musculus

text: Mus musculus C57BL/6J YAC URA3 Alu


characteristic: INSDC last update

text: live

text: Generic

text: Rsa digest

text: Mus musculus

text: Mus musculus C57BL/6J YAC URA3 Rsa


text: live

text: Generic

text: Rsa digest

text: Mus spretus

text: Mus spretus YAC TRP1 Rsa


characteristic: INSDC secondary accession

text: live

text: Generic

characteristic: SRA accession

text: Rsa digest

text: Mus spretus

text: Mus spretus YAC URA3 Rsa


text: Institute of Hematology and Blood Transfusion

text: live

text: Generic

characteristic: NCBI submission model

text: Homo sapiens

text: Exon trapping from 5q31 between IL-9 and testican


text: Guy's Hospital

text: live

text: Generic

characteristic: NCBI submission package

text: Homo sapiens

text: D. Nizetic at ICRF, London


text: Istituto di Ricerche Farmacologiche 'Mario Negri'

text: live

text: Generic

text: ALU-PCR fragment obtained with ALU4 primer

characteristic: description

text: Homo sapiens

text: blood

text: Homo sapiens blood ALU-PCR


text: live

text: Generic

text: primary marginal zone B-cell lymphoma

characteristic: cell type

text: Homo sapiens

text: skin

text: Homo sapiens skin ALU-PCR


text: NDSU

text: live

text: Generic

text: Sus scrofa

characteristic: organism

text: Sus scrofa RDA


text: Medical College of Ohio

text: live

text: Generic

text: Rattus norvegicus

text: Rattus norvegicus DNA

characteristic: title


text: UMDS, Guy's Hospital

text: live

text: Generic

text: Homo sapiens

text: ICRF Reference Library c109


text: Novartis AG

text: live

text: Generic

text: PCR-amplified

text: Funneliformis mosseae

text: external hyphae

text: Glomus mosseae external hyphae BEG12


text: Academic Medical Centre

text: live

text: Generic

text: Homo sapiens

text: human total DNA


text: University of Utah

text: live

text: Generic

text: Purified genomic DNA from M. musculus C57BL/6J (male) was obtained from the Jackson Laboratory Mouse DNA Resource (http://www.jax.org/resources/documents/dnares/). The DNA was hydrodynamically sheared by repeated passage through a 0.005 inch orifice at constant velocity. The sheared DNA was blunt end-repaired with T4 DNA polymerase and T4 polynucleotide kinase. Adaptor oligonucleotides were ligated to the blunt ends in high molar excess. The adaptored DNA was purified and size-selected for a 9.5 to 10.5 kb range using preparative agarose gel electrophoresis. Vector DNA was prepared from a derivative of pWD42 (gi|4732114|gb|AF129072.1), a copy-number inducible derivative of plasmid R1. The vector was ligated with adaptors complementary to the insert adaptors and purified. The sheared, adaptored mouse DNA was annealed to adaptored vector DNA, and transformed into chemically-competent E. coli XL10-Gold (Stratagene) cells and selected for ampicillin resistance.

text: E. Coli strain XL10-Gold, T1-resistant, F-

characteristic: lab-host

text: Mus musculus

text: Male

characteristic: sex

text: Mouse 10kb plasmid UUGC1M library

text: Plasmid

characteristic: vector


characteristic: External Id

text: National University of Singapore

text: live

text: Generic

text: isolated from T-DNA insertion of antisense MEFE5

text: Brassica juncea

text: Brassica juncea DNA (Lim TS)


text: Institution of Genetics, Chinese Academy of Science

text: live

text: Generic

text: Hordeum vulgare

text: Hordeum vulgare DNA (Huang Z)


text: University of Milan

text: live

text: Generic

text: mono-chromosomal cell hybrid panel. From Resources for Molecular Cytogenetics University of Bari-Italy

text: Homo sapiens

text: mono-chromosomal cell hybrid panel, Resources for Molecular Cytogenetics University of Bari-Italy


text: Univ. of Tokyo

text: live

text: Generic

text: Oryzias latipes

text: HNI

characteristic: strain

text: Oryzias latipes HNI


text: Univ. of Tokyo

characteristic: INSDC center name

text: live

text: Generic

text: Oryzias latipes

text: female

text: HNI

text: Oryzias latipes HNI female


text: Univ. of Tokyo

text: live

characteristic: INSDC status

text: Generic

text: Oryzias latipes

text: male

text: HNI

text: Oryzias latipes HNI male


text: Univ. of Tokyo

characteristic: INSDC first public

text: live

text: Generic

text: Oryzias latipes

text: female

text: Oryzias latipes AA2 female


text: Univ. of Tokyo

characteristic: INSDC last update

text: live

text: Generic

text: Oryzias latipes

text: male

text: Oryzias latipes AA2 male


text: Jawaharlal Nehru University

text: live

text: Generic

text: Normal B-lymphoblastoid cell line

characteristic: cell-line

text: Homo sapiens

text: Homo sapiens Normal B-lymphoblastoid cell line

text: pMOS Blue


text: Jawaharlal Nehru University

text: live

text: Generic

text: lymphocyte

text: Homo sapiens

text: Homo sapiens lymphocyte (Bamezai R)

text: pMOS Blue


text: Jawaharlal Nehru University

text: live

text: Generic

text: Bloom syndrome B-lymphoblastoid cell line with normal Sister chromatid exchanges

text: Homo sapiens

text: pMOS Blue


text: Jawaharlal Nehru University

text: live

text: Generic

text: Bloom syndrome B-lymphoblastoid cell line with high Sister chromatid exchanges

text: Homo sapiens

text: pMOS Blue


text: Technical University of Gdansk

text: live

text: Generic

text: Thermus thermophilus HB8

text: Thermus thermophilus HB-8

text: type strain of Thermus thermophilus

characteristic: type material


text: Shimane Medical University

text: live

text: Generic

characteristic: NCBI submission model

text: Rattus norvegicus

text: Wistar-Kyoto

text: Rattus norvegicus Wistar-Kyoto


text: University of Otago

text: live

text: Generic

characteristic: NCBI submission package

text: Ovis aries

text: MSCJL01 (S.M. Galloway)


text: live

text: Generic

text: Coxiella burnetii

text: Priscilla

text: Coxiella burnetii Priscilla


text: live

text: Generic

text: Coxiella burnetii

text: Nine Mile

text: Coxiella burnetii Nine Mile


text: NN Petrov Institute of Oncology

text: live

text: Generic

text: peripheral blood lymphocytes

text: Homo sapiens

text: Homo sapiens library (Fedorov SN)


text: Gifu University School of Medicine

text: live

text: Generic

text: Trichinella pseudospiralis

text: Trichinella pseudospiralis DNA (Wu Z)


text: IRTA

text: live

text: Generic

text: Helicoverpa armigera

text: Helicoverpa armigera RAPD


text: IRTA

text: live

text: Generic

text: Trialeurodes vaporariorum

text: Trialeurodes vaporariorum RAPD


text: University of Nevada, Las Vegas

text: live

text: Generic

text: Homo sapiens

text: lung carcinoma

characteristic: tissue-type

text: Homo sapiens A549


text: University of British Columbia

text: live

text: Generic

text: Homo sapiens

text: LA08NC01 flow sorted chromosome 8-specific cosmid library


text: Stanford University

text: live

text: Generic

text: Toxoplasma gondii

text: PLK

text: Toxoplasma gondii PLK


text: University of Technology Sydney

text: live

text: Generic

text: Aeromonas hydrophila

text: Aeromonas hydrophila ATCC7966


text: University of Birmingham

text: live

text: Generic

text: Pseudomonas aeruginosa

text: PAO1 (ATCC15692)

text: Pseudomonas aeruginosa PAO1 (ATCC15692)


text: Beth Israel Hospital

text: live

text: Generic

text: Brugia malayi

text: provided by Fran Perler


text: University of Delaware

text: live

text: Generic

text: Plumatella emarginata

text: Plumatella emarginata DNA


text: St. Paul Ramsey Medical Center

text: live

text: Generic

text: sequence was obtained from a clinical isolate deficient of the normal IS6110 gene; organism is sensitive to T2H and is pyrazinamidase positive

characteristic: description

text: Mycobacterium tuberculosis

text: Mycobacterium tuberculosis from clinical isolate


text: University di Urbino

text: live

text: Generic

text: Tuber borchii

characteristic: organism

text: Tuber borchii DNA


text: Korean Institute of Tuberculosis

text: live

text: Generic

text: Mycobacterium tuberculosis

text: cosmid 53R

characteristic: title


text: Institute of Science and Technology for Research and Development

text: live

text: Generic

text: Plasmodium falciparum

text: Kanchanaburi (K1)

text: Plasmodium falciparum Kanchanaburi (K1)


text: Roswell Park Cancer Inst.

text: live

text: Generic

text: amplified from YAC DNA using TTAGGG oligomer as primer

text: Mus musculus

text: Princeton library


text: Kansas State University

text: live

text: Generic

text: serotype 2

text: Streptococcus suis

text: Streptococcus suis 3977B


text: Korean Institute of Tuberculosis

text: live

text: Generic

text: Mycobacterium tuberculosis

text: cosmid 10R


text: Institute of Genetic Ecology, Tohoku University

text: live

text: Generic

text: Pectobacterium carotovorum

text: Erwinia carotovora DNA (Chuang D-Y)


text: MDC Berlin-Buch

text: live

text: Generic

text: Bos taurus

text: Bos taurus DNA (Vogel T)


text: MDC Berlin-Buch

text: live

text: Generic

text: adult

characteristic: dev-stage

text: Bos taurus

text: male

text: testes

text: Bos taurus testes adult


text: Pathology

text: live

text: Generic

text: gel purified band from a PCR reaction; template was extracted genomic DNA; sequencing primer=TCAACCACACCACGGGCTTT

text: Schizosaccharomyces pombe

text: PCR with primers specific for human CD44 exon 9 and 16


text: Pathology

text: live

text: Generic

text: PCR-amplified using primers specific for human CD44 exons 9 and 16

text: Hordeum vulgare subsp. vulgare

text: bomi

text: Hordeum vulgare bomi


text: Centers for Disease Control and Prevention

text: live

text: Generic

text: isolate=Jax

text: Ehrlichia chaffeensis

text: Ehrlichia chaffeensis Jax variable length PCR target


text: live

text: Generic

text: isolate=St. Vincent

text: Ehrlichia chaffeensis

text: Ehrlichia chaffeensis St. Vincent variable length PCR target


text: Monash University

text: live

text: Generic

text: Shigella flexneri

text: Shigella flexneri serotype 2a strain SBA1336


text: Universite Lyon1

text: live

text: Generic

text: sequence obtained by random cloning of total DNA from Azospirillum lipoferum CRT1

text: Azospirillum lipoferum

text: Azospirillum lipoferum CRT1


text: University of North Carolina at Chapel Hill

text: live

text: Generic

text: from cosmid 80B7, Lawrence Berkeley National Laboratory

text: Homo sapiens

text: Human cosmid 80B7


text: Leiden University

text: live

text: Generic

text: from C80, a random cosmid that was isolated from a library constructed from a hamster-human hybrid cell line; this hybrid contains a translocated chromosome 5pcen-p15.1::4p15.1-4pter (J.J. Wasmuth, Am. J. Hum. Genet. 39, 397-403, 1986); also contained within cosmid c145e5 (S. Baxendale, Nature Genet. 4, 181-186, 1993

text: Homo sapiens

text: Homo sapiens HHW693


text: Leiden University

text: live

text: Generic

text: from cosmid B117; cloned from YAC 302C8 generated by Alu-PCR using primer A1S (A.R. Brooks-Wilson, Genomics 7, 614-620, 1990); YAC 302C8 was isolated from the CEPH YAC library (H.M. Albertsen, Proc. Natl. Acad. Sci. USA 87, 4256-4260, 1990) using C80H1 from GenBank Accession Number AF059508

text: Homo sapiens

text: Homo sapiens DNA from cosmid B117


text: Universidade Federal de Minas Gerais

text: live

text: Generic

text: PBMN

characteristic: cell type

text: from infectious anemia virus infected horses

text: Equus caballus

text: Equus caballus PBMN


text: University of Wisconsin-Milwaukee

text: live

text: Generic

text: Vibrio mytili

text: NCIMB 13275

text: Vibrio mytili NCIMB 13275


text: University of Wisconsin-Milwaukee

text: live

text: Generic

text: Vibrio pelagius

text: ATCC 25916

text: Listonella pelagia ATCC 25916


text: University of Wisconsin-Milwaukee

text: live

text: Generic

text: Pseudoalteromonas undina

text: ATCC 29660

text: Pseudoalteromonas undina ATCC 29660


text: Johns Hopkins University

text: live

text: Generic

text: Mus musculus

text: SM/J

text: Mus musculus SM/J


text: live

text: Generic

text: isolated by subtractive hybridization with Culex pipiens quinquefasciatus genomic DNA

text: Culex pipiens pipiens

text: Culex pipiens pipiens DNA


text: Vrije Universiteit Brussel

text: live

text: Generic

characteristic: cultivar

text: Arabidopsis thaliana

text: Arabidopsis thaliana C24


characteristic: External Id

text: Inje Med. College

text: live

text: Generic

text: child

text: Homo sapiens

text: hepatitis B virus-infected kidney

text: Child hepatitis B virus-infected kidney


text: National Institute of Immunology

text: live

text: Generic

text: Bubalus bubalis

text: Bubalus arnee bubalis DNA


text: Institute of Biomedical Sciences

text: live

text: Generic

text: Leishmania major

text: Leishmania major ORF-containing genomic fragments


text: Washington University School of Medicine

text: live

text: Generic

text: Inter-Alu amplification from YAC clones yWXD490, yWXD720 and yWXD1441 (Center for Genetics Washington University, St. Louis)

text: Homo sapiens

text: YAC clones Inter-Alu amplification


text: University of Hawaii

text: live

text: Generic

text: Bactrocera dorsalis

text: Puna

characteristic: strain

text: Bactrocera dorsalis Puna


text: Texas A&M University

characteristic: INSDC center name

text: live

text: Generic

text: Gossypium hirsutum

text: Gossypium hirsutum RAPD marker


text: Clemson University

text: live

characteristic: INSDC status

text: Generic

text: AY; Ananas Yokneum

text: Cucumis melo

text: Cucumis melo Ananas Yokneum


text: University of Sheffield

characteristic: INSDC first public

text: live

text: Generic

text: Staphylococcus aureus

text: Staphylococcus aureus 8325-4


text: Indian Institute of Science

characteristic: INSDC last update

text: live

text: Generic

text: Planococcus lilacinus

text: cockeril

text: Planococcus lilacinus cockeril


text: DVA West LA Medical Center

text: live

text: Generic

text: Rattus norvegicus

text: brain

text: Rattus norvegicus brain


text: University of Texas Southwestern Medical Center

text: live

text: Generic

text: Mus musculus

text: BAC library, Research Genetics catalog number 96022


text: International Institute of Tropical Agriculture

text: live

text: Generic

text: Vigna unguiculata

text: Vigna unguiculata IT84E-124


text: Nara Women's University

text: live

text: Generic

text: Nand II

text: Blastocystis hominis

text: Blastocystis hominis ATCC50177


text: Nara Women's University

text: live

text: Generic

text: isolated from a patient in 1994

text: Blastocystis hominis

text: B

text: Blastocystis hominis B


text: Colorado State University

text: live

text: Generic

text: exon trap product from cosmid LSBC29.1; cosmid LSBC29.1 contains LF178 cDNA RFLP locus and maps at 26% FLpter on chromosome 1

text: Aedes aegypti

text: Aedes aegypti cosmid LSBC29.1


text: Forbio Research Pty LTD

text: live

text: Generic

text: country=Australia:Indooroopilly, Brisbase

text: Eucalyptus grandis

text: Eucalyptus grandis RAPD


text: USDA ARS PSI

text: live

text: Generic

text: Williams 82 (PI 518671)

text: EcoRI fragments were gel isolated and cloned into pUC18

characteristic: lab-host

text: Glycine max

text: EcoRI

text: Williams 82 BAC Gm_ISb001_056_G02 EcoRI subclone library

text: Plasmid

characteristic: vector


text: National Institute on Deafness and Other Communication Disorders, National Institutes of Health

text: live

text: Generic

characteristic: NCBI submission model

text: Male C57BL/6J genomic DNA was partially digested with Sau3AI, ligated to ds linkers, and enriched for binding to murine Pax3dQ+ protein using a Whole Genome PCR-based strategy. DNA fragments containing putative Pax3dQ+ binding sites were amplified by PCR and cloned into pGEM-T Easy (Promega). The ligation products were transformed into DH10B electrocompetent cells (Life Technologies).

text: Mus musculus

text: Male

characteristic: sex

text: Pax3 CASTing Library 'cq'

text: plasmid

text: pGEM-T Easy


text: live

text: Generic

characteristic: NCBI submission package

text: Human genomic DNA was partially digested with Sau3AI, ligated to ds linkers, and enriched for binding to human PAX3/FKHR protein using a Whole Genome PCR-based strategy. DNA fragments containing putative PAX3/FKHR binding sites were amplified by PCR and cloned into pGEM-T Easy (Promega). The ligation products were transformed into DH10B electrocompetent cells (Life Technologies).

text: Homo sapiens

text: Male

text: PAX3/FKHR CASTing Library 'ew'

text: plasmid

text: pGEM-T Easy


text: live

text: Generic

text: Human genomic DNA was partially digested with Sau3AI, ligated to ds linkers, and enriched for binding to human PAX3dQ+ protein using a Whole Genome PCR-based strategy. DNA fragments containing putative PAX3dQ+ binding sites were amplified by PCR and cloned into pGEM-T Easy (Promega). The ligation products were transformed into DH10B electrocompetent cells (Life Technologies).

characteristic: description

text: Homo sapiens

text: Male

text: PAX3 CASTing Library 'ev'

text: plasmid

text: pGEM-T Easy


text: Children's Hospital Oakland Research Institute

text: live

text: Generic

text: Lymphocyte

text: BAC clones in E. coli DH10B

text: Homo sapiens

characteristic: organism

text: Male

text: RPCI-11 Human segment 1-4 genomic library


text: live

text: Generic

text: Lymphocyte

text: BAC clones in E. coli DH10B

text: Homo sapiens

text: Male

text: RPCI-11 Human segment 5 genomic library

characteristic: title


text: University of Leuven

text: live

text: Generic

text: Homo sapiens

text: RPCI-6 PAC library


text: Niigata Univ. School of Medicine

text: live

text: Generic

text: Mus musculus molossinus

text: Mus musculus molossinus DNA


text: Department of Genetics, The Hospital for Sick Children

text: live

text: Generic

text: Homo sapiens

text: Homo sapiens DNA (Scherer SW)


text: University of Missouri

text: live

text: Generic

text: PstI digested B73 genomic sucrose gradient size fractionated fragment sizes of 0.5kb to 2kb ligated to PUC19 transformed in DH5 alpha

text: DH5 alpha

text: Leaf

characteristic: organ

text: Zea mays

text: Leaf

characteristic: tissue-type

text: Maize PstI B73 Leaf

text: Plasmid


text: Ben-Gurion University of The Negev, The Jacob Blaustein Institute for desert Research

text: live

text: Generic

text: flowering

characteristic: dev-stage

text: leaf

text: Mercurialis annua

text: male

text: includes all tissues of the leaf

text: Mercurialis annua Genomic

text: plasmid

text: pT-Adv.


characteristic: External Id

text: live

text: Generic

text: Homo sapiens

text: Chromosome 7-specific Yeast Artificial Chromosome Library (YAC HSC7E947)


text: Michigan State University

text: live

text: Generic

text: Sinorhizobium meliloti

characteristic: strain

text: Sinorhizobium meliloti 1021


text: Cold Spring Harbor Laboratory

characteristic: INSDC center name

text: live

text: Generic

characteristic: cultivar

text: DNA prepared from purified nuclei was digested with the methylation insensitive enzyme Spe I, size fractionated to enrich for the 0.5 to 4 kpb fraction, ligated into Xba I digested M13 vector and electroporated into E.coli JM107.

text: immature ears

text: Zea mays

text: Xba I

text: JM107 filtered library

text: Phage


text: Chulalongkorn University

characteristic: INSDC first public

text: live

characteristic: INSDC status

text: Generic

text: Homo sapiens

text: Male

text: White blood cell and sperm

text: Homo sapiens genomic DNA random plasmid subclone for CpG island

text: plasmid

text: pPCR-Script Cam SK(+) plasmid


text: University of California, Davis

characteristic: INSDC last update

text: live

text: Generic

text: Deltistes luxatus

text: Deltistes luxatus DNA


text: University of California, Davis

text: live

text: Generic

text: Catostomus rimiculus

text: Catostomus rimiculus DNA


text: University of California, Davis

text: live

text: Generic

text: Catostomus snyderi

text: Catostomus snyderi DNA


text: University of Glasgow

text: live

text: Generic

text: Plasmodium chabaudi

text: Plasmodium chabaudi DNA


text: North Carolina State University

text: live

text: Generic

text: Nostoc sp. PCC 7120 = FACHB-418

text: BamHI

text: BamHI

text: Library of Sau3AI genomic fragments

text: Plasmid

text: pBlueScript

characteristic: vector


text: Washington University Medical School

text: live

text: Generic

characteristic: NCBI submission model

text: Random genomic sequence

text: Saccharomyces bayanus

text: MCYC 623-6C

text: Saccharomyces bayanus MCYC 623-6C

text: Phage


text: Washington University Medical School

text: live

text: Generic

text: Random genomic sequence

text: Saccharomyces cariocanus

text: UFRJ 50791

text: Saccharomyces cariocanus UFRJ 50791

text: Phage


text: Washington University Medical School

text: live

text: Generic

text: Random genomic sequence

text: Saccharomyces mikatae

text: IFO 1815

text: Saccharomyces mikatae IFO 1815

text: Phage


text: Washington University Medical School

text: live

text: Generic

text: Random genomic sequence

text: Saccharomyces paradoxus

text: Saccharomyces paradoxus N17

text: Phage


text: Washington University Medical School

text: live

text: Generic

text: Random genomic sequence

text: Naumovozyma castellii

text: NRRL Y-12630 (CBS 4309)

text: Saccharomyces castellii NRRL Y-12630

text: Phage


text: University of Ulsan College of Medicine

text: live

text: Generic

text: lymphocytes

characteristic: cell type

text: PAC clones in E. coli DH10B. For PAC library availability, please contact Pieter de Jong (BACPACorders@mail.cho.org). Clones may be purchased from BACPAC Resources (www.chori.org/bacpac) or from Research Genetics (info@resgen.com)

text: E. coli DH10B

characteristic: lab-host

text: Homo sapiens

text: male

characteristic: sex


text: Universidad de Valencia

text: live

text: Generic

characteristic: NCBI submission package

text: single strand sequence;~ PCR product from the reverse transcriptase domain of a 'bilbo' element

characteristic: description

text: Drosophila subobscura

characteristic: organism

text: Drosophila subobscura bilbo element reverse transcriptase


text: Universidad de Valencia

text: live

text: Generic

text: Drosophila obscura

text: Drosophila obscura bilbo element reverse transcriptase

characteristic: title


text: Universidad de Valencia

text: live

text: Generic

text: Drosophila bifasciata

text: Drosophila bifasciata bilbo element reverse transcriptase


text: Universidad de Valencia

text: live

text: Generic

text: Drosophila azteca

text: Drosophila azteca bilbo element reverse transcriptase


text: Universidad de Valencia

text: live

text: Generic

text: Drosophila algonquin

text: Drosophila algonquin bilbo element reverse transcriptase


text: Universidad de Valencia

text: live

text: Generic

text: Drosophila miranda

text: Drosophila miranda bilbo element reverse transcriptase


text: Universidad de Valencia

text: live

text: Generic

text: Drosophila pseudoobscura

text: Drosophila pseudoobscura bilbo element reverse transcriptase


text: University of Toronto at Mississauga

text: live

text: Generic

text: Armillaria gallica

text: Armillaria gallica paMbo38c


text: University of Toronto at Mississauga

text: live

text: Generic

text: Armillaria gallica

text: Armillaria gallica paMbo41f


text: University of Toronto at Mississauga

text: live

text: Generic

text: Armillaria gallica

text: Armillaria gallica paMbo48c


text: University of Toronto at Mississauga

text: live

text: Generic

text: Armillaria gallica

text: Armillaria gallica paMbo52f


text: University of Toronto at Mississauga

text: live

text: Generic

text: Armillaria gallica

text: Armillaria gallica paMbo63r


text: University of Toronto at Mississauga

text: live

text: Generic

text: Armillaria gallica

text: Armillaria gallica paMbo67c


text: University of Toronto at Mississauga

text: live

text: Generic

text: Armillaria gallica

text: Armillaria gallica B05c


text: University of Toronto at Mississauga

text: live

text: Generic

text: Armillaria gallica

text: Armillaria gallica C04f


text: University of Toronto at Mississauga

text: live

text: Generic

text: Armillaria gallica

text: Armillaria gallica C06f


text: University of Toronto at Mississauga

text: live

text: Generic

text: Armillaria gallica

text: Armillaria gallica C15f


text: University of Toronto at Mississauga

text: live

text: Generic

text: Armillaria gallica

text: Armillaria gallica C33r


text: University of Toronto at Mississauga

text: live

text: Generic

text: Armillaria gallica

text: Armillaria gallica E16f


text: University of Toronto at Mississauga

text: live

text: Generic

text: Armillaria gallica

text: Armillaria gallica E26f


text: University of Toronto at Mississauga

text: live

text: Generic

text: Armillaria gallica

text: Armillaria gallica E75c


text: University of Toronto at Mississauga

text: live

text: Generic

text: Armillaria gallica

text: Armillaria gallica E88c


text: University of Toronto at Mississauga

text: live

text: Generic

text: Armillaria gallica

text: Armillaria gallica H81c


text: University of Toronto at Mississauga

text: live

text: Generic

text: Armillaria gallica

text: Armillaria gallica I58f


text: University of Toronto at Mississauga

text: live

text: Generic

text: Armillaria gallica

text: Armillaria gallica I70f


text: University of Toronto at Mississauga

text: live

text: Generic

text: Armillaria gallica

text: Armillaria gallica I75f


text: University of Toronto at Mississauga

text: live

text: Generic

text: Armillaria gallica

text: Armillaria gallica I84c


text: University of Toronto at Mississauga

text: live

text: Generic

text: Armillaria gallica

text: Armillaria gallica J1c


text: University of Toronto at Mississauga

text: live

text: Generic

text: Armillaria gallica

text: Armillaria gallica M62c


text: University of Toronto at Mississauga

text: live

text: Generic

text: Armillaria gallica

text: Armillaria gallica N45c


text: University of Toronto at Mississauga

text: live

text: Generic

text: Armillaria gallica

text: Armillaria gallica O17f


text: University of Toronto at Mississauga

text: live

text: Generic

text: Armillaria gallica

text: Armillaria gallica Q59c


text: University of Toronto at Mississauga

text: live

text: Generic

text: Armillaria gallica

text: Armillaria gallica S34f


text: University of Toronto at Mississauga

text: live

text: Generic

text: Armillaria gallica

text: Armillaria gallica S92r


text: University of Toronto at Mississauga

text: live

text: Generic

text: Armillaria gallica

text: Armillaria gallica T83f


text: University of Toronto at Mississauga

text: live

text: Generic

text: Armillaria gallica

text: Armillaria gallica V19r


text: University of Toronto at Mississauga

text: live

text: Generic

text: Armillaria gallica

text: Armillaria gallica V28f


text: University of Toronto at Mississauga

text: live

text: Generic

text: Armillaria gallica

text: Armillaria gallica V43r


text: University of Toronto at Mississauga

text: live

text: Generic

text: Armillaria gallica

text: Armillaria gallica V46r


text: University of Toronto at Mississauga

text: live

text: Generic

text: Armillaria gallica

text: Armillaria gallica W40f


text: University of Toronto at Mississauga

text: live

text: Generic

text: Armillaria gallica

text: Armillaria gallica W68r


text: University of Toronto at Mississauga

text: live

text: Generic

text: Armillaria gallica

text: Armillaria gallica X82r


text: University of Toronto at Mississauga

text: live

text: Generic

text: Armillaria gallica

text: Armillaria gallica Y38f


text: University of Toronto at Mississauga

text: live

text: Generic

text: Armillaria gallica

text: Armillaria gallica Y72f


text: Shizuoka University

text: live

text: Generic

text: embryo

characteristic: dev-stage

text: Coturnix coturnix

text: Coturnix coturnix embryo


characteristic: External Id

text: Radiation Effects Research Foundation

text: live

text: Generic

text: Mus musculus

text: Mus musculus NotI/HinfI DNA fragment


text: Ji Lin University, Department of Molecular Biology

text: live

text: Generic

text: authority=Cervus nippon Temminck

text: Cervus nippon

text: male

text: liver

characteristic: tissue-type

text: Deer liver library


text: Khon Kaen University

characteristic: INSDC center name

text: live

text: Generic

text: Genomic DNA of P. heterotremus was extracted from the adult worms collected from experimentally infected cats by the proteinase K/SDS method. The genomic DNA was then completely digested with restriction enzyme ACC I. The resulting DNA fragments were purified and ligated into the ACC I site of pBluescript KS+ plasmid (Stratagene, La Jolla, California). The recombinant plasmid DNA was transform into Escherichia coli XL1- blue (Stratagene) using an eletroporation method. Escherichia coli clones containing the recombinant plasmids were screened by hybridization with 32P-labeled total P. heterotremus genomic DNA. The most strongly hybridizing recombinant plasmid that may contain repetitive DNA sequences of P. heterotremus (referred to as pPH-13) was obtained. Finally, the pPH-13 was digested with restriction enzyme ACC I to get the inserted piece (approximately 1.5 kb) and was 32P labeled and tested for its specificity and sensitivity for detection of P. heterotremus DNA and evaluated for its diagnostic potential. Total genomic DNA used for specificity testing was prepared from a number of other parasites, namely, G. spinigerum, O. viverini, E. malayanum, T. spiralis, D. immitis, A. suum, F. gigantica, and T saginata. Additional DNA samples were also extracted from several genera of bacterai, namely, E. coli, Burkholderia pseudomallei, Acinetobacter anitratus, and M. tuberculosis and from the vertebrate and invertebrate host of P. heterotremus (human, cat, crabs, and snails).

text: Adult

text: Whole adult body

characteristic: organ

text: Paragonimus heterotremus

text: ACC I

text: ACC I

text: Hermaphrodite

text: Wild-type

characteristic: strain

text: Paragonimus heterotremus genomic DNA libraries

text: Phagemid

text: pBluescript KS


text: School of Basic Medical Sciences, Ji Lin University

characteristic: INSDC first public

text: live

characteristic: INSDC status

text: Generic

text: authority=Cervus nippon Temminck

text: Cervus nippon

text: male

text: tonsil

text: Deer tonsil library


text: Clemson University

characteristic: INSDC last update

text: live

text: Generic

text: E. coli

text: Bradyrhizobium japonicum

text: HindIII

text: B. japonicum BAC library

text: BAC

characteristic: vector


text: University of Utah

text: live

text: Generic

characteristic: NCBI submission model

text: Purified genomic DNA from M. musculus C57BL/6J (female) was obtained from the Jackson Laboratory Mouse DNA Resource (http://www.jax.org/resources/documents/dnares/). The DNA was hydrodynamically sheared by repeated passage through a 0.005 inch orifice at constant velocity. The sheared DNA was blunt end-repaired with T4 DNA polymerase and T4 polynucleotide kinase. Adaptor oligonucleotides were ligated to the blunt ends in high molar excess. The adaptored DNA was purified and size-selected for a 9.5 to 10.5 kb range using preparative agarose gel electrophoresis. Vector DNA was prepared from a derivative of pWD42 (gi|4732114|gb|AF129072.1), a copy-number inducible derivative of plasmid R1. The vector was ligated with adaptors complementary to the insert adaptors and purified. The sheared, adaptored mouse DNA was annealed to adaptored vector DNA, and transformed into chemically-competent E. coli XL10-Gold (Stratagene) cells and selected for ampicillin resistance.

text: E. coli strain XL10-Gold, T1-resistant, F-

text: Mus musculus

text: Female

text: Mouse 10kb plasmid UUGC2M library

text: Plasmid


text: University of Berne

text: live

text: Generic

text: Bos taurus

text: Bos taurus PCR product


text: University of Florida

text: live

text: Generic

text: Genomic DNA was prepared from asynchronous blood stage forms of the Belem line of P. vivax grown in squirrel monkeys. Parasitized erythrocytes were purified from contaminating host leukocytes by filtration of ADP activated blood through acid-washed glass beads and Whatman CF11 cellulose columns by gravity filtration. Purified DNA was digested with mung bean nuclease in the presence of 42.5% formamide at 50oC as described (Galinski, M. et al. 1992. Cell 69,1213-1226; Vernick, K.D. et al.1988. N.A.R. 16, 6883-6896). Eco RI linkers were added and the constructs ligated into Lambda ZAP II. P. vivax Belem was originally isolated from a patient in Belem, Brazil 1980 by Mercia de Arruda, adapted to Saimiri monkeys by Jurg Gysin, and maintained since 1983 in squirrel monkeys.

text: asexual blood forms

text: Saimiri boliviensis

characteristic: lab-host

text: Plasmodium vivax

text: EcoR I

text: EcoR I

text: Belem

text: Pv MBN #16 (amplified twice)

text: phagemid

text: Lambda ZAP II (Stratagene); individual clones excised into phagemid pBluescript


text: University of Florida

text: live

text: Generic

text: asexual blood forms

text: Saimiri boliviensis

text: Plasmodium vivax

text: EcoR I

text: EcoR I

text: Belem

text: Pv MBN #26/#27 (amplified once)

text: phagemid


text: Nankai University

text: live

text: Generic

text: Cycas changjiangensis N. Liu

text: Cycas changjiangensis

text: young leaf

text: Cycas changjiangensis RAPD differential clone


text: Texas A&M University

text: live

text: Generic

text: genotype A17

characteristic: cultivar

text: Unpublished second HindIII BAC library (MT-H2) of Medicago truncatula.

text: Medicago truncatula

text: HindIII

text: HindIII

text: Medicago truncatula MT-H2 BAC library


text: United States Environmental Protection Agency

text: live

text: Generic

characteristic: NCBI submission package

characteristic: cell-line

text: DNA was isolated from UOK123 cells after sodium arsenite exposure for 4 weeks. The fragment was differentially methylated relative to untreated controls and was identified using methylation sensitive AP-PCR and sequenced.

text: Kidney

text: Homo sapiens

text: Human kidney cell line UOK123 after sodium arsenite exposure


text: live

text: Generic

text: DNA was isolated from UOK109 cells after sodium arsenite exposure for 4 weeks. This fragment was differentially methylated relative to untreated controls and was identified using methylation sensitive AP-PCR and sequenced.

characteristic: description

text: Kidney

text: Homo sapiens

characteristic: organism

text: Human kidney cell line UOK109 after sodium arsenite exposure


text: live

text: Generic

text: DNA was isolated from UOK121 cells after sodium arsenite exposure for 4 weeks. This fragment was differentially methylated relative to untreated controls and was identified using methylation sensitive AP-PCR and sequenced.

text: Kidney

text: Homo sapiens

text: Human kidney cell line UOK121 after sodium arsenite exposure

characteristic: title


characteristic: External Id

text: University of Florida

text: live

text: Generic

text: Host leukocytes were extracted from P. vivax infected blood using the following methods: first, infected blood was activated by the addition of 0.5 ml of ADP (40mg/ml) per 10 ml blood. Then blood was passed over a column of acid washed 0.1 mm glass beads, then through a Plasmodipur filter, followed by passage through a column of pre-wet Whatman CF11 powder (1:2 ratio volume of blood to CF11), and finally centrifuged through a 50% Percoll density cushion. Purified DNA was digested with mung bean nuclease in the presence of 44% formamide at 50oC as described (Vernick, K.D., Imberski, R.B., and McCutchan, T.F. 1988. Nucleic Acids Research 16:6883-6896). Digested DNA was blunt-ended using T4 DNA Polymerase and size fractionated over a Sepharose CL-2B column. Fractions in the size range 500bp-4kb were ligated into the Eco RV site of pBluescript SK(+), and E. coli XL-10 Gold transformed with the ligation mixture.

text: asexual blood forms

characteristic: dev-stage

text: Saimiri boliviensis

text: Plasmodium vivax

text: EcoR V

text: EcoR V

text: Salvador I (Collins, W. 1972. J. Parasitol. 69, 497-598)

text: Pv MBN #30

text: phagemid

text: pBluescript SK(+) vector DNA, phagemid excised from lambda ZAP


text: Seattle Biomedical Research Institute

characteristic: INSDC center name

text: live

text: Generic

text: Genomic DNA from Leishmania major Friedlin was partially digested with Sau3AI, size selected, and ligated with BamHI-digested cLHYG cosmid vector DNA. 9216 clones were picked and arrayed. Library construction is described in Ivens et al., Genomics Research, 8:135-145 (1998). The cLHYG vector (Acc. No. CVU59231) is described in Ryan et al, Gene, 131:145-150 (1993)

text: E. coli ED8767

text: Leishmania major

text: BamHI

text: Friedlin

text: Leishmania major Friedlin Cosmid Genomic Library

text: Cosmid

text: cLHYG


text: Johns Hopkins University School of Medicine

text: live

text: Generic

text: Homo sapiens

text: Human chromosome 18q21 from exon-trapping


text: College of Life Sciences, Henan Normal University

text: live

text: Generic

text: adult

text: Cyprinus carpio

text: male

characteristic: sex

text: blood

characteristic: tissue-type

text: Cyprinus carpio adult blood

text: Plasmid

text: T-vector


characteristic: INSDC first public

text: live

text: Generic

text: Pseudois nayaur

text: Pseudois nayaur RAPD


text: Emporia State University

text: live

characteristic: INSDC status

text: Generic

text: Genomic DNA was digested with BamH1 and randomly cloned into BamH1 digested pBluescript.

text: Lespedeza cuneata

text: Genomic library from Lespedeza cuneata


characteristic: INSDC last update

text: live

text: Generic

text: DNA was isolated from A549 cells after sodium arsenite exposure for 4 weeks. This fragment was differentially methylated relative to untreated controls and was identified using methylation sensitive AP-PCR and sequenced.

text: Lung

characteristic: organ

text: Homo sapiens

text: Human lung cell line A549 after sodium arsenite exposure


text: University of Turin

text: live

text: Generic

characteristic: NCBI submission model

text: Fusarium oxysporum f. sp. basilici

text: Fusarium oxysporum f. sp. basilici DNA


text: Massachusetts Institute of Technology

text: live

text: Generic

text: Homo sapiens

text: male

text: DOP-PCR 8BJ from human genomic DNA

text: Plasmid

text: pCR2.1 TA

characteristic: vector


text: Massachusetts Institute of Technology

text: live

text: Generic

text: Mus musculus

characteristic: strain

text: DOP-PCR 10C from mouse genomic DNA

text: Plasmid

text: pCR-XL-TOPO


text: Massachusetts Institute of Technology

text: live

text: Generic

characteristic: NCBI submission package

text: Columbia

characteristic: cultivar

text: Arabidopsis thaliana

text: DOP-PCR 9C from Arabidopsis genomic DNA

text: Plasmid

text: pCR-XL-TOPO


text: C.N.R.S.

text: live

text: Generic

text: Jemalong

text: Medicago truncatula

text: Medicago truncatula Jemalong BAC genomic


text: Sr Barholomew's and Royal London School of Medicine

text: live

text: Generic

text: isolate=Moredun Institute cervine

characteristic: description

text: Cryptosporidium parvum

text: Cryptosporidium parvum Moredun Institute cervine


text: Genoscope - Centre National de Sequencage

text: live

text: Generic

text: Zygosaccharomyces rouxii

characteristic: organism

text: CBS 732


text: Mahidol University

text: live

text: Generic

text: Homo sapiens

text: Homo sapiens DNA (Akkarapatumwong V)

characteristic: title


characteristic: External Id

text: Swedish University of Agricultural Sciences

text: live

text: Generic

text: Genomic library of the plant pathogenic fungus Verticillium dahliae. Both fungal DNA and the plasmid pUC18 were digested to completion with EcoRI. Ligation and transformation into the E. coli DH5a strain followed standard procedures.

text: E. coli DH5a

characteristic: lab-host

text: Verticillium dahliae

text: EcoRI

text: EcoRI

text: Fungal hyphae and microsclerotia

text: Plasmid


text: Torrey Pines Institute for Molecular Studies

characteristic: INSDC center name

text: live

text: Generic

text: Embryonic stem cell line

characteristic: cell type

characteristic: cell-line

text: Available from Research Genetics (www.ResGen.com); note - strain of origin actually 129S1/Sv, immediate ancestor of 129S3/SvImJ; for description of 129 substrains see Simpson et al. (1997) Nature Genetics 16: 19-27 and http://www.informatics.jax.org/mgihome/nomen/strain_129.shtml

text: HS996 (modified DH10B)

text: Mus musculus

text: HindIII

text: CitbCJ7 mouse BAC library

text: BAC


text: University of Queensland

characteristic: INSDC first public

text: live

text: Generic

text: Purified genomic DNA from Gemmata obscuriglobus was restricted with Sau3AI to give fragments of 400-3000bp and ligated into the BamHI site of the pBluescript II SK(-) phagemid. The ligated DNA was electroporated into E. coli XL1-Blue.

text: Escherichia coli strain XL1-Blue

text: Gemmata obscuriglobus

text: BamHI

text: BamHI

text: ACM 2246

text: Gemmata obscuriglobus phagemid library

text: plasmid

text: pBluescript II SK(-) phagemid


text: Meiji Pharmaceutical University

text: live

characteristic: INSDC status

text: Generic

text: Trichosporon asahii

text: Trichosporon asahii U-2


text: Clemson University

characteristic: INSDC last update

text: live

text: Generic

text: Maxxa

text: For more details on library preparation, ordering clones and sequence analysis see http://www.genome.clemson.edu/projects/stc/cotton/GH_MBb

text: E. coli

text: Gossypium hirsutum

text: HindIII

text: NotI

text: Young leaves

characteristic: tissue-type

text: Gossypium hirsutum L.

text: Plasmid


text: Emporia State University

text: live

text: Generic

characteristic: NCBI submission model

text: Genomic DNA was digested with EcoR1 and randomly cloned into EcoR1 digested pBluescript.

text: Salmonella enterica subsp. enterica serovar Derby

text: Genomic library from Salmonella derby


text: Emporia State University

text: live

text: Generic

text: Salmonella enterica subsp. enterica serovar Typhimurium

text: Genomic library from Salmonella typhimurium


text: Emporia State University

text: live

text: Generic

text: Salmonella enterica subsp. enterica serovar Pullorum

text: Genomic library from Salmonella pullorum


text: Emporia State University

text: live

text: Generic

text: Salmonella enterica subsp. enterica serovar Reading

text: Genomic library from Salmonella reading


text: Washington University School of Medicine

text: live

text: Generic

text: Homo sapiens

text: Research Genetics Human BAC library, Releases 3 & 4

text: BAC


text: IRTA

text: live

text: Generic

text: synonym: Prunus amygdalus

text: Prunus dulcis

text: Almond pBluescript genomic DNA Library


text: IRTA

text: live

text: Generic

text: Prunus ferganensis

text: Prunus ferganensis Genomic DNA Library


text: IRTA

text: live

text: Generic

text: Prunus domestica

text: Plum Genomic DNA Library


text: IRTA

text: live

text: Generic

text: m27 rootstock

text: Malus domestica

text: Apple Genomic DNA Library


text: Institute of Experimental Cardiology, Cardiology Research Center

text: live

text: Generic

text: ATCC26012; 2-5 kb fraction of DNA fragments obtained by partial digestion of H. polymorpha DL-1 chromosomal DNA with Sau3A was ligated with XhoI-digested pKE32 vector. Two bp of the cohesive DNA ends were preliminary filled-in with Klenow enzyme. The XhoI site locates upstream to the sequence encoding B. subtilis CMCase (D01057). Besides the reporter sequence encoding the CMCase, the vector bears the LEU2 gene and the fragment of 2um DNA of S. cerevisiae, that allows its stable autonomous maintenance in H. polymorpha CBS4732 cells.

text: Ogataea angusta

text: XhoI 2 bp filled-in

text: H. polymorpha DL-1 leu2 mutant DL1-L

text: H. polymorpha DL1 expression library

text: Plasmid

characteristic: vector


text: Genoscope - Centre National de Sequencage

text: live

text: Generic

text: Yarrowia lipolytica

text: CLIB 89

characteristic: strain


text: live

text: Generic

characteristic: NCBI submission package

text: Lachancea thermotolerans

text: CBS 6340


text: live

text: Generic

text: Kluyveromyces lactis

text: CLIB 210


text: live

text: Generic

text: Ogataea angusta

text: CBS 4732


text: live

text: Generic

text: Candida tropicalis

text: CBS 94


text: Centre de Genetique Moleculaire, Centre National de la Recherche Scientifique

text: live

text: Generic

text: macronuclear sequence; The present survey of the Paramecium tetraurelia macronuclear genome consists of end sequences of a library of random 4-12 kb fragments obtained by Sau3A partial digestion of macronuclear DNA cloned in the BamHI site of pBSIIKS-.

text: Paramecium tetraurelia

text: stock d4-2

text: Paramecium tetraurelia stock d4-2


text: Institut Pasteur

text: live

text: Generic

text: Mus musculus

text: CITB Mouse BAC Library


text: Institut Pasteur

text: live

text: Generic

text: Mus musculus

text: Female

characteristic: sex

text: RPCI-23 Mouse BAC Library


text: The Institute for Genomic Research

text: live

text: Generic

text: Spleen/Brain

text: RPCI-24 Mouse BAC Library produced by Pieter de Jong. The library was cloned in the pTARBAC1 cloning vector at the BamH1 sites using MboI partially digested male C57BL/6J DNA.

characteristic: description

text: Mus musculus

text: Male

text: BAC


text: USDA-ARS

text: live

text: Generic

text: SSR-containing subclone of soybean BAC - described by Cregan et al. 1999. Targeted isolation of simple sequence repeat markers through the use of bacterial artificial chromosomes. Theor. Appl. Genet. 98:919-928

text: Glycine max

characteristic: organism

text: Soybean BAC subclone


text: Dupont Genomics

text: live

text: Generic

text: Young leaf

text: Total genomic DNA was nebulized; ends were polished with Pfu polymerase and the fragments cloned into pCR-Script.

text: seedling

characteristic: dev-stage

text: Zea mays

text: hermaphrodite

text: leaf

text: Maize Random Small-insert Genomic Library

characteristic: title

text: Plasmid

text: pCR-Script


characteristic: External Id

text: Queen's University of Belfast

text: live

text: Generic

text: lymphocytes

text: RPCI Human PAC libraries have been constructed by P.de Jong and his group at the Roswell Park Cancer Institute, Buffalo. Anonymous male blood DNA was isolated, partially digested with MboI and cloned into the BamH1 sites of the pCYPAC2 vector. The ligation products were transformed into DH10B electrocompetent cells (BRL Life technologies). Further information about the library can be found at http://www.chori.org/bacpac/. Clones sequenced in this project were obtained from UK HGMP-RC, Hinxton, UK.

text: Homo sapiens

text: male

text: Human PAC RPI1 library

text: PAC


text: Queen's University of Belfast

characteristic: INSDC center name

text: live

text: Generic

text: Human YAC library constructed at ICRF as described by Lorin et al (1991) PNAS 88:4123-4127. The library combines three separate libraries: 1.4X (human female lymphoblastoid 48XXXX cell line): 15,700 clones; average insert size 600 Kb, 3x coverage of autosomes and 6x coverage of X. 2.4Y (human male lymphoblastoid cell line 49XYYYY): 2300 clones; insert size 400-500 Kb. 3.HD (human female lymphoblastoid, Huntingdon's disease 46XX): 2500 clones; average insert size 600 Kb.

text: Homo sapiens

text: Human ICRF YAC library

text: YAC


text: Queen's University of Belfast

text: live

text: Generic

text: The chromosome 17 library was constructed at Los Alamos National Laboratory according to the method described in Longmire, JL. et al Genetic Analysis, Techniques and Applications, 1993, 10:69-76. It has an average insert size of 30 - 40 Kb and contains approx. 15,300 clones providing a 5-fold coverage of the chromosome. The vector used is sCOS1 (Evans, GA. et al Gene 1989 79: 9-20, sequence) in E. coli strain HB101. The library consists of 40 plates labelled 1-40.

text: Homo sapiens

text: cosmid


text: Pearl River Fisheries Research Institute

text: live

text: Generic

text: Siniperca chuatsi virus isolated from cultured mandarin fish, Sin iperca chuatsi(Basilewsky).

text: Siniperca chuatsi virus

text: SCV RAPD sequences

text: pGEM-T easy


text: Oregon State University

text: live

text: Generic

text: mycelia

characteristic: cell type

text: Pyrenophora tritici-repentis

text: BFP

text: 3.0 Mb chromosome-specific subtraction library


text: Emporia State University

text: live

text: Generic

text: Genomic DNA was digested with EcoR1 and randomly cloned into EcoR1 digested pBluescript.

text: Lespedeza cuneata

text: Genomic library from Lespedeza cuneata EcoR1


text: Clemson University

text: live

text: Generic

characteristic: cultivar

text: For more details on library preparation, ordering clones and sequence analysis see http://www.genome.clemson.edu/projects/stc/maize/ZMMBBb

text: E. coli

characteristic: lab-host

text: Zea mays

text: HindIII

text: NotI

text: Young leaves

text: Maize B73

text: Plasmid


text: University Hospital of Zurich

characteristic: INSDC first public

text: live

characteristic: INSDC status

text: Generic

text: Mus musculus

text: direct sequencing of pcr product (from genomic DNA)


text: Virginia Tech

characteristic: INSDC last update

text: live

text: Generic

text: Bos taurus

text: Bos taurus genomic DNA (Smith, EJ)


text: Virginia Tech

text: live

text: Generic

characteristic: NCBI submission model

text: Homo sapiens

text: Homo sapiens genomic DNA (Smith, EJ)


text: Virginia Tech

text: live

text: Generic

text: Sus scrofa

text: Sus scrofa genomic DNA (Smith, EJ)


text: IACR-Rothamsted

text: live

text: Generic

text: Puccinia striiformis f. sp. tritici

text: Puccinia striiformis f. sp. tritici DNA


text: Deutsches Krebsforschungszentrum

text: live

text: Generic

text: Homo sapiens


text: Clemson University

text: live

text: Generic

text: Ochrobactrum anthropi is an important microbe having potential for the bioremediation of environments contaminated by aliphatic compounds. We have constructed a BAC library for O. anthropi that provides a 90x genome coverage based on an estimated genome size of 3.83 Mb. The library contains 3072 clones with an average insert size of 112 kbp. High-density colony filters of the library were made and a physical map of the genome constructed using a hybridization without replacement strategy. In addition, 624 randomly chosen BAC clones were HindIII fingerprinted and anylized using Fingerprint Contig (FPC; Sangre Centre, UK). The FPC results closely supported and verified the hybridization contig data. After deterimining a reduced tiling path of 69 clones, 138 BAC ends were sequenced for a genome wide survey of gene distribution and gene structure.

text: DH10B E. coli

text: Brucella anthropi

text: HindIII

text: HindIII

text: Ochrobactrum anthropi BAC Library

text: BAC

characteristic: vector


text: University of Cambridge

text: live

text: Generic

text: Genomic DNA 3' to the P-element insertion site was recovered using inverse PCR (protocol details can be found at: http://www.fruitfly.org/about/methods/inverse.pcr.html ). The isolated sequence was cloned into a pGEM-T vector and sequenced from the T7 primer.

text: Drosophila melanogaster

text: Drosophila melanogaster P-element line: l(2)k13715


text: University of Cambridge

text: live

text: Generic

text: Drosophila melanogaster

text: Drosophila melanogaster P-element line: l(2)k09314


text: University of Hong Kong

text: live

text: Generic

text: Panax ginseng

text: Panax ginseng DNA, low cot