text: Washington University School of Medicine

text: live

text: Generic

text: Construction of the cDNA library was carried out using Stratagenes 'UniZAP - cDNA synthesis kit'. cDNA was constructed using an oligo dT primer/linker that contains a XhoI site within it. Following ds cDNA synthesis, EcoRI adapters were ligated to the blunt ends and sample was digested with XhoI. The result is cDNA with an EcoRI sticky end on one side and a XhoI sticky end on the other. This cDNA was ligated directionally in UniZAP arms. The vector is designed containing the pBluescript sequence as well as lambda DNA and cDNA is cloned within this pBluescript sequence. The vector was then packaged using Gold gigapackaging extracts. Library was grown in XLIBlue MRF' cells and amplified. The library was excised by mass excision using Stratagens 'Mass excision kit' that uses exassist as a helper phage that releases the pBluescript sequence and circularises it as single stranded plasmids that are then packaged (by helper phage) and secreted out of the host cell as phagemids. SOLR cells were transformed with phagemids and the library was plated out on LB-amp plates to select for transformants. Approximately 1,000,000 colonies were grown and recovered. The double stranded plasmid library was recovered by using Quiagen Midi prep kit. 2 micro grams of each library were used to transform DH10B cells by electroporation.

text: Ceratodon purpureus

characteristic: organism

text: EcoRI

text: XhoI

text: protonemata: 7 day old tissue

text: Moss EST library CPU

text: phagemid (ampicillin resistant)

text: pBluescript SK-


status: PUBLIC

characteristic: External Id

text: Indian Agricultural Research Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: 20-45 DAF

tag: attribute

characteristic: dev-stage

text: Brassica juncea

text: variety Pusa Jaikishan

characteristic: strain

text: developing seed

characteristic: tissue-type

text: Brassica juncea developing seed 20-45 DAF

characteristic: title


text: live

text: Generic

text: Anchor enzyme: NlaIII~ This SAGE library is from primary tumor, serous adenocarcinoma. 'Velculescu, V. E., Zhang, L., Vogelstein, B., and Kinzler, K. W. (1995) Serial Analysis of Gene Expression. Science 270, 484-487'

characteristic: description

text: Homo sapiens

text: primary tumor, serous adenocarcinoma


text: Christian-Albrects University of Kiel

text: live

text: Generic

text: Buessow, K. et al., 1998, Nucleic Acids Research 26(21): 5007-5008

text: fetal

text: Homo sapiens

text: brain

text: hEx1 library


text: California Institute of Technology

text: live

text: Generic

text: early cleavage stage embryo

text: Strongylocentrotus purpuratus

text: Strongylocentrotus purpuratus early cleavage stage embryo


text: Peking Union Medical College; Chinese Academy of Medical Science

text: live

text: Generic

text: 8-12 weeks

text: Rattus norvegicus

text: male

characteristic: sex

text: Sprague-Dawley

text: quadrups skeletal muscles

text: Rat skeletal muscle,Clontech


text: Clemson University

text: live

text: Generic

text: Rio Grande PtoR

characteristic: cultivar

text: cLET - Inoculated with a variety of disease response elicitors. Plants exposed to 2,6 dichloroisonicotinic acid, BTH, jasmonic acid, ethylene, fenthion, EIX, okadaic acid, or systemin prior to tissue harvest. EcoRI site was destroyed during cloning.

text: 4-6 week old plants

text: XL1-Blue MRF'

characteristic: lab-host

text: Solanum lycopersicum

text: leaf

text: tomato mixed elicitor, BTI

text: phagemid

text: pBlueScript SK(-)

characteristic: vector


text: Saitama University

text: live

text: Generic

text: embryo

text: Rattus norvegicus

characteristic: organism

text: brain

text: Rattus norvegicus embryo brain


characteristic: External Id

text: Medical Institute of Bioregulation, Kyushu University

text: live

text: Generic

text: ovarian cancer

characteristic: cell type

text: skov

characteristic: cell-line

text: Homo sapiens

text: Homo sapiens skov


text: Shiga University of Medical Science

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: hybrid cell of Hela and fibroblast; sequence obtained by differential display between tumorigenic and non-tumorigenic cell hybrids

text: Homo sapiens

text: Homo sapiens CGL3


text: University of Georgia

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: E. coli

text: Gossypium arboreum

text: EcoRI

text: XhoI

text: AKA

text: Fibers isolated from bolls harvested 7-10 dpa

text: Gossypium arboreum 7-10 dpa fiber library

text: Plasmid

text: pBK-CMV


text: live

text: Generic

text: Anchor enzyme: NlaIII~ This SAGE library is from Tumor type: Human microvascular endothelial cells(HMVEC) harvested at passage 5. 'Velculescu, V. E., Zhang, L., Vogelstein, B., and Kinzler, K. W. (1995) Serial Analysis of Gene Expression. Science 270, 484-487'

text: Homo sapiens

text: microvascular endothelial cells (HMVEC) harvested at passage 5

characteristic: tissue-type


text: live

text: Generic

text: Anchor enzyme: NlaIII~ This SAGE library is from Tumor type: Human microvascular endothelial cells (HMVEC) treated with Vascular endothelial growth factor (VEGF) from Clonetics harvested at passage 5. 'Velculescu, V. E., Zhang, L., Vogelstein, B., and Kinzler, K. W. (1995) Serial Analysis of Gene Expression. Science 270, 484-487'

text: Homo sapiens

text: microvascular endothelial cells (HMVEC) treated with VEGF harvested at passage 5

characteristic: title


text: University of Wisconsin

text: live

text: Generic

text: First strand cDNA was primed with a NotI-polyT primer [5'-AACTGGAAGAATTCGCGGCCGCAGGAATTTTTTTTTTTTTTTTTT-3']. Double-stranded cDNA was ligated with EcoRI adapters (Pharmacia), digested with NotI, and ligated into the EcoRI/NotI sites of the pGEM11Zf vector. The library was NOT normalized.

characteristic: description

text: liver

characteristic: organ

text: Mus musculus

text: NotI

text: EcoRI

text: male

characteristic: strain

text: mouse liver, dioxin treated

text: plasmid

text: pGEM11Zf (Promega)


text: University of Wisconsin

text: live

text: Generic

characteristic: lab-host

text: liver

text: Mus musculus

text: NotI

text: EcoRI

text: male

characteristic: sex

text: mouse liver, vehicle control

text: plasmid

text: pGEM11Zf (Promega)


text: University of Guelph

text: live

text: Generic

text: stage 20

characteristic: dev-stage

text: Gallus gallus

characteristic: organism

text: embryo

text: Gallus gallus embryo stage 20


characteristic: External Id

text: University of Western Ontario

text: live

text: Generic

text: approx. 1 year old animal

text: Oncorhynchus mykiss

text: female

text: ovary

text: Rainbow trout RT-PCR product

text: Plasmid

text: pGEM-T (Promega)

characteristic: vector


text: University of Technology, Sydney

characteristic: INSDC center name

text: live

text: Generic

text: Tachyzoite

characteristic: cell type

text: Neospora caninum

text: NC-Liverpool

text: Neospora caninum Lambda Zap cDNA library


text: Stanford University

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

characteristic: cultivar

text: cDNA library from fully differentiated maize tissues from an active Mutator plant. Tissue ratio is 4/2/1/1/1/1 (tassel, kernel, silk, husk, root, leaf). Unidirectionally cloned.

text: adult

text: tassel, kernel, silk, husk, root, leaf

text: Zea mays

text: EcoRI

text: tassel, kernel, silk, husk, root, leaf

text: 707 - Mixed adult tissues from Walbot lab (SK)

text: plasmid


text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: The NIH_BMAP_M_S3.2 library is a subtracted library of a series, ultimately derived from a mixture of individually tagged normalized libraries from ten regions of the mouse brain (cerebellum, brain stems, olfactory bulbs, hypothalamus, cortex, amygdala, basal ganglia, pineal gland, striatum, hipoccampus) after a series of subtractions to reduce the representation of cDNAs from which ESTs had already been generated. The following serially subtracted libraries were generated in this process: NIH_BMAP_M_S3.2, NIH_BMAP_M_S2, NIH_BMAP_M_S1. The subtracted library (NIH_BMAP_M_S3.2) was constructed as follows: PCRamplified cDNA inserts from NIH_BMAP_M_S2 clones from which 3' ESTs had been derived was used as a driver in a hybridization with the NIH_BMAP_M_S2 library in the form of single-stranded circles. The remaining single-stranded circles (subtracted library) was purified by hydroxyapatite column chromatography, converted to double-stranded circles and electroporated into DH10B bacteria (LifeTechnologies) to generate the NIH_BMAP_M_S3.2 library. This procedure has been previously described (Bonaldo, Lennon and Soares, Genome Research 6:791-806, 1996)

text: 27-32 days

text: DH10B (Life Technologies)

text: Mus musculus

text: Not I

text: Eco RI

text: Phagemid


text: live

text: Generic

text: The NIH_BMAP_M_S3.3 library is a subtracted library of a series, ultimately derived from a mixture of individually tagged normalized libraries from ten regions of the mouse brain (cerebellum, brain stems, olfactory bulbs, hypothalamus, cortex, amygdala, basal ganglia, pineal gland, striatum, hipoccampus) after a series of subtractions to reduce the representation of cDNAs from which ESTs had already been generated. The following serially subtracted libraries were generated in this process: NIH_BMAP_M_S3.3, NIH_BMAP_M_S2, NIH_BMAP_M_S1. The subtracted library (NIH_BMAP_M_S3.3) was constructed as follows: PCRamplified cDNA inserts from NIH_BMAP_M_S2 clones from which 3' ESTs had been derived was used as a driver in a hybridization with the NIH_BMAP_M_S2 library in the form of single-stranded circles. The remaining single-stranded circles (subtracted library) was purified by hydroxyapatite column chromatography, converted to double-stranded circles and electroporated into DH10B bacteria (LifeTechnologies) to generate the NIH_BMAP_M_S3.3 library. This procedure has been previously described (Bonaldo, Lennon and Soares, Genome Research 6:791-806, 1996)

characteristic: description

text: 27-32 days

text: DH10B (Life Technologies)

text: Mus musculus

text: Not I

text: Eco RI

characteristic: title

text: Phagemid


text: live

text: Generic

text: The NIH_BMAP_M_S3.1 library is a subtracted library of a series, ultimately derived from a mixture of individually tagged normalized libraries from ten regions of the mouse brain (cerebellum, brain stems, olfactory bulbs, hypothalamus, cortex, amygdala, basal ganglia, pineal gland, striatum, hipoccampus) after a series of subtractions to reduce the representation of cDNAs from which ESTs had already been generated. The following serially subtracted libraries were generated in this process: NIH_BMAP_M_S3.1, NIH_BMAP_M_S2, NIH_BMAP_M_S1. The subtracted library (NIH_BMAP_M_S3.1) was constructed as follows: PCRamplified cDNA inserts from NIH_BMAP_M_S2 clones from which 3' ESTs had been derived was used as a driver in a hybridization with the NIH_BMAP_M_S2 library in the form of single-stranded circles. The remaining single-stranded circles (subtracted library) was purified by hydroxyapatite column chromatography, converted to double-stranded circles and electroporated into DH10B bacteria (LifeTechnologies) to generate the NIH_BMAP_M_S3.1 library. This procedure has been previously described (Bonaldo, Lennon and Soares, Genome Research 6:791-806, 1996)

text: 27-32 days

text: DH10B (Life Technologies)

text: Mus musculus

text: Not I

text: Eco RI

characteristic: strain

text: Phagemid


text: live

text: Generic

text: Anchor enzyme: NlaIII~ This SAGE library is from Tumor type: Brain, medulloblastoma tumor. The tumor was a 4th ventricular mass, of unknown c-myc status. 'Velculescu, V. E., Zhang, L., Vogelstein, B., and Kinzler, K. W. (1995) Serial Analysis of Gene Expression. Science 270, 484-487'

text: Brain

characteristic: organ

text: Homo sapiens

text: medulloblastoma tumor

characteristic: tissue-type


text: live

text: Generic

text: Anchor enzyme: NlaIII~ This SAGE library is from Tumor type: Normal human luminar mammary epithelial cells purified with Epithelial Enrich (BER-EP4 antibody conjugated to magnetic beads). 'Velculescu, V. E., Zhang, L., Vogelstein, B., and Kinzler, K. W. (1995) Serial Analysis of Gene Expression. Science 270, 484-487'

text: Homo sapiens


text: The Samuel Roberts Noble Foundation

text: live

text: Generic

text: Jemalong, line A17

text: At the trifoliate stage, M. truncatula plants were transplanted to phosphate-free sand and grown for a further 30 days. During this 30 day period, they were fertilized twice weekly with 1/2 Hoaglands solution containing only 20uM potassium phosphate. RNA was prepared from the roots. cDNA was prepared from polyA+ enriched RNA. The cDNA was directionally ligated into the Unizap XR vector from Stratagene and packaged using Gigapack III Gold packaging extracts. Plasmids containing cDNA inserts were excised from the recombinant lambda-Zap phage using Ex-assist helper phage and propagated in SOLR cells.

text: phosphate starved

characteristic: dev-stage

text: Medicago truncatula

text: EcoRI

text: XhoI

text: roots

text: rootphos(-)

text: Plasmid

text: pBluescript SK -


text: Washington University School of Medicine

text: live

text: Generic

text: Construction of the cDNA library was carried out using Stratagenes 'UniZAP - cDNA synthesis kit'. cDNA was constructed using an oligo dT primer/linker that contains a XhoI site within it. Following ds cDNA synthesis, EcoRI adapters were ligated to the blunt ends and sample was digested with XhoI. The result is cDNA with an EcoRI sticky end on one side and a XhoI sticky end on the other. This cDNA was ligated directionally in UniZAP arms. The vector is designed containing the pBluescript sequence as well as lambda DNA and cDNA is cloned within this pBluescript sequence. The vector was then packaged using Gold gigapackaging extracts. Library was grown in XLIBlue MRF' cells and amplified. The library was excised by mass excision using Stratagens 'Mass excision kit' that uses exassist as a helper phage that releases the pBluescript sequence and circularises it as single stranded plasmids that are then packaged (by helper phage) and secreted out of the host cell as phagemids. SOLR cells were transformed with phagemids and the library was plated out on LB-amp plates to select for transformants. Approximately 1,000,000 colonies were grown and recovered. The double stranded plasmid library was recovered by using Quiagen Midi prep kit. 2 micro grams of each library were used to transform DH10B cells by electroporation.

characteristic: lab-host

text: Physcomitrium patens

characteristic: organism

text: EcoRI

text: XhoI

text: protonemata: 7 day old tissue ammonium-grown

text: Moss EST library PPU

text: phagemid (ampicillin resistant)

text: pBluescript SK-


characteristic: External Id

text: Oklahoma State University

text: live

text: Generic

text: Neoaliturus tenellus

text: Circulifer tenellus library (Chandrasekaran A)


text: Yonsei University College of Medicine

characteristic: INSDC center name

text: live

text: Generic

text: 11.5 d.p.c.

text: Mus musculus

text: Mus musculus 11.5 d.p.c.


text: University of Minnesota

text: live

text: Generic

text: genotype A17

text: cDNA was prepared from polyA+ enriched RNA from effective root nodules harvested one month post inoculation with Sinorhizobium meliloti. The cDNA was directionally ligated into the Uni-ZAP XR vector from Stratagene and packaged using Gigapack III Gold packaging extracts. Plasmids containing cDNA inserts were excised from the recombinant lambda-ZAP phage using Ex-Assist helper phage and propagated in XLOLR cells.

text: E. coli strain XLOLR

text: Medicago truncatula

text: EcoRI

text: XhoI

text: N2-fixing root nodules

text: GVN

text: Plasmid

text: pBluescript SK-

characteristic: vector


text: University of Minnesota

characteristic: INSDC first public

text: live

text: Generic

text: genotype A17

text: cDNA was prepared from polyA+ enriched RNA from the mixture of cotyledons of five days old plants and leaves of two weeks old plants. The cDNA was directionally ligated into the Uni-ZAP XR vector from Stratagene and packaged using Gigapack III Gold packaging extracts. Plasmids containing cDNA inserts were excised from the recombinant lambda-ZAP phage using Ex-Assist helper phage and propagated in SOLR cells.

text: mixture of cotyledons from five days old plants and leaves obtained from two weeks old plants

text: E. coli strain SOLR

text: Medicago truncatula

text: EcoRI

text: XhoI

text: leaves and cotyledons

text: DSLC

text: Plasmid

text: pBluescript SK +/-


text: University of Minnesota

characteristic: INSDC last update

text: live

text: Generic

text: genotype A17

text: cDNA was prepared from polyA+ enriched RNA from cotyledons and primary leaves harvested 5 and 8 days after inoculation with Colletotrichum trifolii. The cDNA was directionally ligated into the Uni-ZAP XR vector from Stratagene and packaged using Gigapack III Gold packaging extracts. Plasmids containing cDNA inserts were excised from the recombinant lambda-ZAP phage using Ex-Assist helper phage and propagated in XLOLR cells. Note: EST may be of fungal origin.

text: E. coli strain XLOLR

text: Medicago truncatula

text: EcoRI

text: XhoI

text: leaves infected with Colletotrichum trifolii

text: DSIL

text: Plasmid

text: pBluescript SK-


text: Institute of Radiation Medicine

text: live

text: Generic

text: fibroblast

characteristic: cell type

text: 0.5 Gy g radiated the cells

text: 3-4 months

text: Homo sapiens

text: male

characteristic: sex

text: lung

text: radiation induced genes library


text: NRIFP

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: uterine decidua from 7 week pregnancy

text: adult

text: Homo sapiens

text: endometrium

text: Homo sapiens endometrium adult


text: University of Tokushima

text: live

text: Generic

characteristic: NCBI submission package

text: Fertilized egg

text: Halocynthia roretzi

text: Halocynthia roretzi Fertilized egg


text: live

text: Generic

text: 1st strand cDNA was prepared from medulloblastoma tumor tissue, and was then primed with a Not I - oligo(dT) primer. Double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. This library is normalized. Library constructed by Bento Soares and M. Fatima Bonaldo.

characteristic: description

text: DH10B (phage resistant)

text: brain

text: Homo sapiens

text: medulloblastoma

characteristic: title

text: phagemid (ampicillin resistant)


text: Chinese Academy of Medical Sciences & Peking Union Medical College

text: live

text: Generic

text: Endothelial cells

text: Homo sapiens

text: Venae

text: Human umbilical venous cord

text: Lambda Zap Express


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: The NCI_CGAP_Sub3 library is a subtracted library derived from the NCI_CGAP_Sub1 library, which is a subtracted library derived from BI. BI constitutes a mixture of 21 normalized or subtracted NCI_CGAP libraries: NCI_CGAP_Co4, NCI_CGAP_Pr22, NCI_CGAP_Pr28, NCI_CGAP_Co10, NCI_CGAP_Co16, NCI_CGAP_Kid5, NCI_CGAP_Kid12, NCI_CGAP_Kid3, NCI_CGAP_Kid11, NCI_CGAP_Lym2, NCI_CGAP_Br2, NCI_CGAP_Co8, NCI_CGAP_CLL1, NCI_CGAP_Lei2, NCI_CGAP_Brn23, NCI_CGAP_Lu5, NCI_CGAP_Lu24, NCI_CGAP_Lu19, NCI_CGAP_GC4, NCI_CGAP_GC6, NCI_CGAP_Brn25. These 21 libraries were pooled and a single-stranded DNA preparation of the resulting mixture was used as a tracer in a subtractive hybridization with a driver whose composition is detailed below: NCI_CGAP_Kid3 pool 1 LLAM 3334-3337, 3682-3683, 3798-3803 (IMAGE CloneIDs 1322376-1323911, 1456008-1456775, 1500552-1502855); NCI_CGAP_Kid5 pool 1 LLAM 3338-3342, 3722-3725, 3776-3778 (IMAGE CloneIDs 1323912-1325831, 1471368-1472903, 1492104-1493255); NCI_CGAP_Lu5 pool 1 LLAM 3575-3582, 3851-3854 (IMAGE CloneIDs 1414920-1417991, 1520904-1522439); NCI_CGAP_GC4 pool 1 LLAM 3164-3167, 3716-3720, 3733-3735 (IMAGE CloneIDs 1257096-1258631, 1469064-1470983, 1475592-1476743); NCI_CGAP_Pr22 pool 1 LLAM 2457-2459, 2758-2759, 3062-3068 (IMAGE CloneIDs 985608-986759, 1101192-1101959, 1217928-1220615); NCI_CGAP_Co10 pool 1 LLAM 2644-2653, 2871-2872 (IMAGE CloneIDs 1057416-1061255, 1144584-1145351). Subtraction was performed as previously described [Bonaldo, Lennon & Soares (1996): Normalization and Subtraction: Two Approaches To Facilitate Gene Discovery. Genome Research 6, 791-806.

text: DH10B (Life Technologies)

text: Homo sapiens

text: Not I

text: Eco RI

text: Phagemid


text: The Institute of Physical and Chemical Research (RIKEN)

text: live

text: Generic

text: cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and Genome Science Laboratory in RIKEN. Division of Experimental Animal Research in Riken contributed to prepare mouse tissues. 1st strand cDNA was primed with a primer [5' GAGAGAGAGAAGGATCCAAGAGCTCTTTTTTTTTTTTTTTTVN 3'], cDNA was prepared by using trehalose thermo-activated reverse transcriptase and subsequently enriched for full-length by cap-trapper. Second strand cDNA was prepared with the primer adapter of sequence [5' GAGAGAGAGAGCGGCCGCAATTAATTCTCGAGTTAATTAAATTAATCCCCCCCCCCC 3']. cDNA was cloned into the XhoI and BamHI sites. Vector: a modified pBluescript KS(+) after bulk excision from Lambda FLC I

text: adult

text: Mus musculus

text: XhoI

text: BamHI

text: male

characteristic: strain

text: cerebellum

text: RIKEN full-length enriched, adult male cerebellum


text: live

text: Generic

text: adult

text: Mus musculus

text: XhoI

text: BamHI

text: female

text: placenta

characteristic: tissue-type

text: RIKEN full-length enriched, adult female placenta


text: live

text: Generic

text: cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and Genome Science Laboratory in RIKEN. Division of Experimental Animal Research in Riken contributed to prepare mouse tissues. 1st strand cDNA was primed with a primer [5' GAGAGAGAGAAGGATCCAAGAGCTCTTTTTTTTTTTTTTTTVN 3'], cDNA was prepared by using trehalose thermo-activated reverse transcriptase and subsequently enriched for full-length by cap-trapper. Second strand cDNA was prepared with the primer adapter of sequence [5' GAGAGAGAGAGCGGCCGCAATTAATTCTCGAGTTAATTAAATTAATCCCCCCCCCCC 3']. cDNA was cloned into the XhoI and BamHI sites.

text: adult

characteristic: dev-stage

text: SOLR

text: Mus musculus

text: XhoI

text: BamHI

text: male

text: testis

text: RIKEN full-length enriched, adult male testis


text: live

text: Generic

text: ES cells

text: cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and Genome Science Laboratory in RIKEN. Division of Experimental Animal Research in Riken contributed to prepare mouse tissues. 1st strand cDNA was primed with a primer [5' GAGAGAGAGAAGGATCCAAGAGCTCTTTTTTTTTTTTTTTTVN 3'], cDNA was prepared by using trehalose thermo-activated reverse transcriptase and subsequently enriched for full-length by cap-trapper. cDNA went through one round of normalization to Rot = 5.0 and subtraction to Rot = 25.0. Second strand cDNA was prepared with the primer adapter of sequence [5' GAGAGAGAGATTCTCGAGTTAATTAAATTAATCCCCCCCCCCCCC 3'].

text: SOLR

characteristic: lab-host

text: Mus musculus

text: XhoI

text: BamHI

text: RIKEN full-length enriched, ES cells


text: live

text: Generic

text: cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and Genome Science Laboratory in RIKEN. Division of Experimental Animal Research in Riken contributed to prepare mouse tissues. 1st strand cDNA was primed with a primer [5' GAGAGAGAGAAGGATCCAAGAGCTCTTTTTTTTTTTTTTTTVN 3'], cDNA was prepared by using trehalose thermo-activated reverse transcriptase and subsequently enriched for full-length by cap-trapper. Second strand cDNA was prepared with the primer adapter of sequence [5' GAGAGAGAGATTCTCGAGTTAATTAAATTAATCCCCCCCCCCCCC 3']

text: adult

text: SOLR

text: Mus musculus

characteristic: organism

text: XhoI

text: BamHI

text: male

text: hippocampus

text: RIKEN full-length enriched, adult male hippocampus


characteristic: External Id

text: live

text: Generic

text: cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and Genome Science Laboratory in RIKEN. Division of Experimental Animal Research in Riken contributed to prepare mouse tissues. 1st strand cDNA was primed with a primer [5' GAGAGAGAGAAGGATCCAAGAGCTCTTTTTTTTTTTTTTTTVN 3'], cDNA was prepared by using trehalose thermo-activated reverse transcriptase and subsequently enriched for full-length by cap-trapper. cDNA went through one round of normalization to Rot = 10.0 and subtraction to Rot = 50.0. Second strand cDNA was prepared with the primer adapter of sequence [5' GAGAGAGAGATTCTCGAGTTAATTAAATTAATCCCCCCCCCCCCC 3']

text: 12 days embryo

text: SOLR

text: Mus musculus

text: XhoI

text: BamHI

text: mixed

text: head

text: RIKEN full-length enriched, 12 days embryo head


characteristic: INSDC center name

text: live

text: Generic

text: cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and Genome Science Laboratory in RIKEN. Division of Experimental Animal Research in Riken contributed to prepare mouse tissues. 1st strand cDNA was primed with a primer [5' GAGAGAGAGAAGGATCCAAGAGCTCTTTTTTTTTTTTTTTTVN 3'], cDNA was prepared by using trehalose thermo-activated reverse transcriptase and subsequently enriched for full-length by cap-trapper. Second strand cDNA was prepared with the primer adapter of sequence [5' GAGAGAGAGATTCTCGAGTTAATTAAATTAATCCCCCCCCCCCCC 3']. cDNA was cloned into the XhoI and BamHI sites. Vector: a modified pBluescript KS(+) after bulk excision from Lambda FLC I

text: 14, 17 days embryo

text: Mus musculus

text: SalI

text: BamHI

text: mixed

text: head

text: RIKEN full-length enriched, 14, 17 days embryo head


text: live

text: Generic

text: 17 days embryo

text: Mus musculus

text: SalI

text: BamHI

text: mixed

text: head

text: RIKEN full-length enriched, 17 days embryo head


text: live

text: Generic

text: 10 days embryo

text: Mus musculus

text: SalI

text: BamHI

text: mixed

text: RIKEN full-length enriched, 10 days embryo


text: live

text: Generic

text: adult

text: Mus musculus

text: SalI

text: BamHI

text: male

text: brain

text: RIKEN full-length enriched, adult male brain


text: live

text: Generic

text: cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and Genome Science Laboratory in RIKEN. Division of Experimental Animal Research in Riken contributed to prepare mouse tissues. 1st strand cDNA was primed with a primer [5' GAGAGAGAGAAGGATCCAAGAGCTCTTTTTTTTTTTTTTTTVN 3'], cDNA was prepared by using trehalose thermo-activated reverse transcriptase and subsequently enriched for full-length by cap-trapper. cDNA went through one round of normalization to Rot = 10.0 and subtraction to Rot = 60.0. Second strand cDNA was prepared with the primer adapter of sequence [5' GAGAGAGAGATTCTCGAGTTAATTAAATTAATCCCCCCCCCCCCC 3']. cDNA was cloned into the XhoI and BamHI sites. Vector: a modified pBluescript KS(+) after bulk excision from Lambda FLC I

text: 12 days embryo

text: Mus musculus

text: SalI

text: BamHI

text: mixed

text: embryonic body below diaphragm region

text: RIKEN full-length enriched, 12 days embryo, body below diaphragm


text: The Institute of Physical and Chemical Research (RIKEN)

text: live

text: Generic

text: cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and Genome Science Laboratory in RIKEN. Division of Experimental Animal Research in Riken contributed to prepare mouse tissues. 1st strand cDNA was primed with a primer [5' GAGAGAGAGAAGGATCCAAGAGCTCTTTTTTTTTTTTTTTTVN 3'], cDNA was prepared by using trehalose thermo-activated reverse transcriptase and subsequently enriched for full-length by cap-trapper. cDNA went through one round of normalization to Rot = 10.0 and subtraction to Rot = 50.0. Second strand cDNA was prepared with the primer adapter of sequence [5' GAGAGAGAGATTCTCGAGTTAATTAAATTAATCCCCCCCCCCCCC 3']. cDNA was cloned into the XhoI and BamHI sites. Vector: a modified pBluescript KS(+) after bulk excision from Lambda FLC I

text: 18 days pregnant, adult

text: Mus musculus

text: SalI

text: BamHI

text: female

text: placenta and extra embryonic tissue

text: RIKEN full-length enriched, 18 days pregnant, placenta and extra embryonic tissue


text: live

text: Generic

text: 13 days embryo

text: Mus musculus

text: SalI

text: BamHI

text: mixed

text: RIKEN full-length enriched, 13 days embryo


text: live

text: Generic

text: 15 days embryo

text: Mus musculus

text: SalI

text: BamHI

text: mixed

text: head

text: RIKEN full-length enriched, 15 days embryo head


text: live

text: Generic

text: 16 days embryo

text: Mus musculus

text: SalI

text: BamHI

text: mixed

text: head

text: RIKEN full-length enriched, 16 days embryo head


text: live

text: Generic

text: 1 day pregnant, adult

text: Mus musculus

text: SalI

text: BamHI

text: female

text: mammary gland

text: RIKEN full-length enriched, 1 day pregnant, mammary gland


text: live

text: Generic

text: cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and Genome Science Laboratory in RIKEN. Division of Experimental Animal Research in Riken contributed to prepare mouse tissues. 1st strand cDNA was primed with a primer [5' GAGAGAGAGAAGGATCCAAGAGCTCTTTTTTTTTTTTTTTTVN 3'], cDNA was prepared by using trehalose thermo-activated reverse transcriptase and subsequently enriched for full-length by cap-trapper. cDNA went through one round of normalization to Rot = 10.0 and subtraction to Rot = 100.0. Second strand cDNA was prepared with the primer adapter of sequence [5' GAGAGAGAGATTCTCGAGTTAATTAAATTAATCCCCCCCCCCCCC 3']. cDNA was cloned into the XhoI and BamHI sites. Vector: a modified pBluescript KS(+) after bulk excision from Lambda FLC I

text: 14 days embryo

text: Mus musculus

text: SalI

text: BamHI

text: mixed

text: liver

text: RIKEN full-length enriched, 14 days embryo liver


text: live

text: Generic

text: 0 day neonate

text: Mus musculus

text: SalI

text: BamHI

text: mixed

text: skin

text: RIKEN full-length enriched, 0 day neonate skin


text: live

text: Generic

text: 10 days neonate

text: Mus musculus

text: SalI

text: BamHI

text: mixed

text: skin

text: RIKEN full-length enriched, 10 day neonate skin


text: Washington University School of Medicine

text: live

text: Generic

text: Construction of the cDNA library was carried out using Stratagenes 'UniZAP - cDNA synthesis kit'. cDNA was constructed using an oligo dT primer/linker that contains a XhoI site within it. Following ds cDNA synthesis, EcoRI adapters were ligated to the blunt ends and sample was digested with XhoI. The result is cDNA with an EcoRI sticky end on one side and a XhoI sticky end on the other. This cDNA was ligated directionally in UniZAP arms. The vector is designed containing the pBluescript sequence as well as lambda DNA and cDNA is cloned within this pBluescript sequence. The vector was then packaged using Gold gigapackaging extracts. Library was grown in XLIBlue MRF' cells and amplified. The library was excised by mass excision using Stratagens 'Mass excision kit' that uses exassist as a helper phage that releases the pBluescript sequence and circularises it as single stranded plasmids that are then packaged (by helper phage) and secreted out of the host cell as phagemids. SOLR cells were transformed with phagemids and the library was plated out on LB-amp plates to select for transformants. Approximately 1,000,000 colonies were grown and recovered. The double stranded plasmid library was recovered by using Quiagen Midi prep kit. 2 micro grams of each library were used to transform DH10B cells by electroporation.

text: Physcomitrium patens

text: EcoRI

text: XhoI

text: protonemata: 7 day old tissue auxin treated

text: Moss EST library PPN

text: phagemid (ampicillin resistant)

text: pBluescript SK-

characteristic: vector


text: Beijing Medical University

characteristic: INSDC first public

text: live

text: Generic

characteristic: cell-line

text: Homo sapiens

text: Homo sapiens u937


text: Cancer Research Institute Hunan Medical University

characteristic: INSDC last update

text: live

text: Generic

text: differential display product obtained by comparing normal laryngeal tissue with laryngeal carcinoma tissue; amplified using primers P9 (5'-attaaccctcactaaatgtggcagg3') and T8 (5'-cattatgctgagtgatatctttttttttgc-3')

text: Homo sapiens

text: Homo sapiens differential display product


text: Wayne State University, School of Medicine

text: live

text: Generic

text: Mus musculus

text: lung mesenchymal cell primary culture

text: Mus musculus lung mesenchymal cell primary culture CRl: CD-1(ICR) BR


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: cDNA was prepared from 2ug of poly A+ RNA. EcoRI-XhoI cut cDNA was then ligated into UniZap-XR (Stratagene) with EcoRI at the 5' end and XhoI at the 3' end. SS-library phagemids were prepared by mass excision from the original library and normalized by hybridization to biotinylated driver (prepared from the same library by PCR) to Cot-omega of 11. After removal of hybrids and excess driver by streptavidin sepharose chromatography, the ss-phagemids were made double stranded and electroporated into Top-10 F'. Original library contruction by Bruce Blumberg (Blumberg et al., 1991 Science 253, 194-196; Hawlet et al., 1995, Genes Dev. 9, 2923-2935). Normalized by Jihwan Song (Song, Cho and Blumberg, unpublished). Note: This is a Xenopus Gene Collection (XGC) library.

text: Top-10 F'

text: Xenopus laevis

text: EcoRI

text: XhoI

text: oocyte (stages 5 and 6)

text: Xenopus laevis oocyte

text: phagemid (ampicillin resistant)

text: pBluescript SK-


text: live

text: Generic

text: cDNA was prepared from 2ug of poly A+ RNA (equal parts from stage 10.5 and stage 11.5 gastrulae). EcoRI-XhoI cut cDNA was then ligated into UniZap-XR (Stratagene) with EcoRI at the 5' end and XhoI at the 3' end. SS-library phagemids were prepared by mass excision from the original library and normalized by hybridization to biotinylated driver (prepared from the same library by PCR) to Cot-omega of 11. After removal of hybrids and excess driver by streptavidin sepharose chromatography, the ss-phagemids were made double stranded and electroporated into Top-10 F'. Original library contruction by Bruce Blumberg (Cho et al. 1991 Cell 67, 1111-1120). Normalized by Jihwan Song (Song, Cho and Blumberg, unpublished). Note: This is a Xenopus Gene Collection (XGC) library.

text: Top-10 F'

text: Xenopus laevis

text: EcoRI

text: XhoI

text: gastrula (stages 10.5, 11.5 mixed)

text: normalized Xenopus laevis gastrula

text: phagemid (ampicillin resistant)

text: pBluescript SK-


text: University of Edinburgh

text: live

text: Generic

text: Litomosoides sigmodontis is a filarial nematode parasite of rats. The library was constructed from vector-(Ornithonyssus bacoti) derived L3 for Prof. David Taylor at the Centre for Tropical Veterinary Medicine, Edinburgh, UK

text: infective L3

text: Litomosoides sigmodontis

text: EcoRI (5'end)

text: XhoI (3'end)

text: mixed

characteristic: sex

text: Litomosoides sigmodontis (parasitic nematode) infective L3

text: phage

text: Lambda Zap II


text: USDA ARS PSI

text: live

characteristic: INSDC status

text: Generic

text: Peking

characteristic: cultivar

text: Glycine max

text: soybean differential display library

text: Plasmid

text: pT7Blue T


text: University of Illinois at Chicago

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: MYCN

characteristic: cell type

text: Homo sapiens

text: neuroblastoma

text: Myosin light chain


text: Clemson University

text: live

text: Generic

text: supplier: Tanksley; Tissue supplied by Dave Garvin (USDA-ARS, Ithaca, NY 14850). Roots were harvested from plants grown under the following deficiencies/stresses: 10 mM Al, Zn, P, K, Fe, N), and mRNA was isolated from indivdual treatments. Proportional aliquots of mRNA of each treatment were mixed and used for library construction.

characteristic: description

text: 5-6 weeks old

text: Solanum lycopersicum

text: 5' EcoRI

text: 3' XhoI

text: roots

text: tomato root deficiency, Cornell University

characteristic: title

text: phagemid

text: pBluescript SK-


text: North Carolina State University

text: live

text: Generic

text: Rice blast-infected leaves at 48 hour post-inoculation mRNA for cDNA library construction.

text: Infection stage at 48 hour post-inoculation

characteristic: dev-stage

text: Oryza sativa/Pyricularia oryzae mixed EST library

characteristic: strain

text: Rice blast infection stage cDNA library

text: Plasmid

text: pBlueScriptII SK(+) Vector


text: University of Chicago Medical Center

text: live

text: Generic

text: Homo sapiens

characteristic: organism

text: small intestine

characteristic: tissue-type

text: cDNA converted from mRNA of normal human small intestine

text: pCR-BLUNT


characteristic: External Id

text: University of Chicago Medical Center

characteristic: INSDC center name

text: live

text: Generic

text: Homo sapiens

text: thymus

text: cDNA converted from thymus mRNA

text: pCR-BLUNT


text: University of Chicago Medical Center

characteristic: INSDC first public

text: live

text: Generic

text: Homo sapiens

text: placenta

text: cDNA converted from placenta mRNA

text: pCR-BLUNT

characteristic: vector


text: University of Chicago Medical Center

characteristic: INSDC last update

text: live

text: Generic

text: Homo sapiens

text: testis

text: cDNA converted from testis mRNA

text: pCR-BLUNT


text: University of Chicago Medical Center

text: live

text: Generic

text: Homo sapiens

text: bone marrow

text: cDNA converted from bone marrow mRNA

text: pCR-BLUNT


text: University of Chicago Medical Center

text: live

text: Generic

text: Homo sapiens

text: trachea

text: cDNA converted from trachea mRNA

text: pCR-BLUNT


text: University of Chicago Medical Center

text: live

text: Generic

text: Homo sapiens

text: colon

text: cDNA converted from colon mRNA

text: pCR-BLUNT


text: The Institute of Physical and Chemical Research (RIKEN)

text: live

text: Generic

text: cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and Genome Science Laboratory in RIKEN. Division of Experimental Animal Research in Riken contributed to prepare mouse tissues. 1st strand cDNA was primed with a primer [5' GAGAGAGAGAAGGATCCAAGAGCTCTTTTTTTTTTTTTTTTVN 3'], cDNA was prepared by using trehalose thermo-activated reverse transcriptase and subsequently enriched for full-length by cap-trapper. cDNA went through one round of normalization to Rot = 10.0 and subtraction to Rot = 100.0. Second strand cDNA was prepared with the primer adapter of sequence [5' GAGAGAGAGATTCTCGAGTTAATTAAATTAATCCCCCCCCCCCCC 3']. cDNA was cloned into the XhoI and BamHI sites. Vector: a modified pBluescript KS(+) after bulk excision from Lambda FLC I.

text: 0 day neonate

characteristic: lab-host

text: Mus musculus

text: SalI

text: BamHI

text: mixed

characteristic: sex

text: head

text: RIKEN full-length enriched, 0 day neonate head


text: live

characteristic: INSDC status

text: Generic

text: cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and Genome Science Laboratory in RIKEN. Division of Experimental Animal Research in Riken contributed to prepare mouse tissues. 1st strand cDNA was primed with a primer [5' GAGAGAGAGAAGGATCCAAGAGCTCTTTTTTTTTTTTTTTTVN 3'], cDNA was prepared by using trehalose thermo-activated reverse transcriptase and subsequently enriched for full-length by cap-trapper. Second strand cDNA was prepared with the primer adapter of sequence [5' GAGAGAGAGATTCTCGAGTTAATTAAATTAATCCCCCCCCCCCCC 3']. cDNA was cloned into the XhoI and BamHI sites. Vector: a modified pBluescript KS(+) after bulk excision from Lambda FLC I. Cloning sites, 5' end: SalI; 3' end: BamHI.

text: adult

text: Mus musculus

text: SalI

text: BamHI

text: male

text: testis

text: RIKEN full-length enriched, adult male testis (DH10B)


text: live

text: Generic

characteristic: NCBI submission model

text: adult

text: Mus musculus

text: SalI

text: BamHI

text: male

text: testis

text: RIKEN full-length enriched, adult male testis (BNN132)


text: live

text: Generic

characteristic: NCBI submission package

text: cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and Genome Science Laboratory in RIKEN. Division of Experimental Animal Research in Riken contributed to prepare mouse tissues. 1st strand cDNA was primed with a primer [5' GAGAGAGAGAAGGATCCAAGAGCTCTTTTTTTTTTTTTTTTVN 3'], cDNA was prepared by using trehalose thermo-activated reverse transcriptase and subsequently enriched for full-length by cap-trapper. cDNA went through one round of normalization to Rot = 10.0 and subtraction to Rot = 100.0. Second strand cDNA was prepared with the primer adapter of sequence [5' GAGAGAGAGATTCTCGAGTTAATTAAATTAATCCCCCCCCCCCCC 3']. cDNA was cloned into the XhoI and BamHI sites. Vector: a modified pBluescript KS(+) after bulk excision from Lambda FLC I. Cloning sites, 5' end: SalI; 3' end: BamHI.

text: adult

text: Mus musculus

text: SalI

text: BamHI

text: male

text: testis

text: RIKEN full-length enriched, adult male testis (DH5a)


text: Cold Spring Harbor Laboratory

text: live

text: Generic

characteristic: cell-line

text: This library was supplied by Holly Cline (Cold Spring Harbor Labs). cDNA synthesis with oligo dT Xba I (Xba I cloning site). RNA: stage 50-56 tadpoles, total brain tissue, GTC extraction method.

text: tadpole

text: Xenopus laevis

text: XbaI

text: total brain tissue

text: Xenopus EST library

text: Lambda Zap I


text: live

text: Generic

characteristic: description

text: adult

text: Mus musculus

text: SalI

text: BamHI

text: male

text: thymus

text: RIKEN full-length enriched, adult male thymus


text: USDA ARS PSI

text: live

text: Generic

text: Century

characteristic: cultivar

text: Glycine max

text: soybean, century cDNA library

characteristic: title

text: Plasmid

text: pBluescript SK-


text: Clemson University

text: live

text: Generic

text: Leaves of various stages were shaken in liquid nitrogen, shearing off trichomes. This procedure yielded a mixture of cells highly enriched for trichomes, with minor contamination by other types of leaf cells.

text: mixed stages

characteristic: dev-stage

text: SOLR

text: Solanum pennellii

text: EcoRI

text: XhoI

text: trichome

text: L. pennellii trichome, Cornell University

text: phagemid

text: pBluescript SK-


text: live

text: Generic

text: 13 days embryo

text: Mus musculus

characteristic: organism

text: SalI

text: BamHI

text: mixed

characteristic: strain

text: forelimb

text: RIKEN full-length enriched, 13 days embryo forelimb


characteristic: External Id

text: live

text: Generic

text: cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and Genome Science Laboratory in RIKEN. Division of Experimental Animal Research in Riken contributed to prepare mouse tissues. 1st strand cDNA was primed with a primer [5' GAGAGAGAGAAGGATCCAAGAGCTCTTTTTTTTTTTTTTTTVN 3'], cDNA was prepared by using trehalose thermo-activated reverse transcriptase and subsequently enriched for full-length by cap-trapper. cDNA went through one round of normalization to Rot = 5.0 and subtraction to Rot = 100.0. Second strand cDNA was prepared with the primer adapter of sequence [5' GAGAGAGAGATTCTCGAGTTAATTAAATTAATCCCCCCCCCCCCC 3']. cDNA was cloned into the XhoI and BamHI sites. Vector: a modified pBluescript KS(+) after bulk excision from Lambda FLC I. Cloning sites, 5' end: SalI; 3' end: BamHI.

text: 13 days embryo

text: Mus musculus

text: SalI

text: BamHI

text: male

text: testis

characteristic: tissue-type

text: RIKEN full-length enriched, 13 days embryo male testis


characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and Genome Science Laboratory in RIKEN. Division of Experimental Animal Research in Riken contributed to prepare mouse tissues. 1st strand cDNA was primed with a primer [5' GAGAGAGAGAAGGATCCAAGAGCTCTTTTTTTTTTTTTTTTVN 3'], cDNA was prepared by using trehalose thermo-activated reverse transcriptase and subsequently enriched for full-length by cap-trapper. cDNA went through one round of subtraction to Rot = 100.0 Second strand cDNA was prepared with the primer adapter of sequence [5' GAGAGAGAGATTCTCGAGTTAATTAAATTAATCCCCCCCCCCCCC 3']. cDNA was cloned into the XhoI and BamHI sites. Vector: a modified pBluescript KS(+) after bulk excision from Lambda FLC I. Cloning sites, 5' end: SalI; 3' end: BamHI.

text: 14 days embryo

text: Mus musculus

text: SalI

text: BamHI

text: mixed

text: thymus

text: RIKEN full-length enriched, 14 days embryo thymus


text: The Institute of Physical and Chemical Research (RIKEN)

characteristic: INSDC last update

text: live

text: Generic

text: 11 days embryo

text: Mus musculus

text: SalI

text: BamHI

text: mixed

text: head

text: RIKEN full-length enriched, 11 days embryo head


text: live

text: Generic

text: 11 days pregnant, adult

characteristic: lab-host

text: Mus musculus

text: SalI

text: BamHI

text: female

text: ovary and uterus

text: RIKEN full-length enriched, 11 days pregnant adult female ovary and uterus


text: Biotechnology Institute of Beijing

text: live

text: Generic

text: thymic stromal cell

characteristic: cell type

text: Mus musculus

text: BALB/c

text: thymus

text: Mouse thymus stromal cell


text: live

characteristic: INSDC status

text: Generic

text: 10 days neonate

text: Mus musculus

text: SalI

text: BamHI

text: mixed

characteristic: sex

text: intestine

text: RIKEN full-length enriched, 10 days neonate intestine


text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and Genome Science Laboratory in RIKEN. Division of Experimental Animal Research in Riken contributed to prepare mouse tissues. 1st strand cDNA was primed with a primer [5' GAGAGAGAGAAGGATCCAAGAGCTCTTTTTTTTTTTTTTTTVN 3'], cDNA was prepared by using trehalose thermo-activated reverse transcriptase and subsequently enriched for full-length by cap-trapper. cDNA went through one round of normalization to Rot = 3.0 and subtraction to Rot = 100.0. Second strand cDNA was prepared with the primer adapter of sequence [5' GAGAGAGAGATTCTCGAGTTAATTAAATTAATCCCCCCCCCCCCC 3']. cDNA was cloned into the XhoI and BamHI sites. Vector: a modified pBluescript KS(+) after bulk excision from Lambda FLC I. Cloning sites, 5' end: SalI; 3' end: BamHI.

text: adult

text: Mus musculus

text: SalI

text: BamHI

text: male

text: xiphoid cartilage

text: RIKEN full-length enriched, adult male xiphoid cartilage


text: live

text: Generic

characteristic: description

text: adult

text: Mus musculus

text: SalI

text: BamHI

text: male

text: pituitary gland

text: RIKEN full-length enriched, adult male pituitary gland


text: live

text: Generic

text: cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and Genome Science Laboratory in RIKEN. Division of Experimental Animal Research in Riken contributed to prepare mouse tissues. 1st strand cDNA was primed with a primer [5' GAGAGAGAGAAGGATCCAAGAGCTCTTTTTTTTTTTTTTTTVN 3'], cDNA was prepared by using trehalose thermo-activated reverse transcriptase and subsequently enriched for full-length by cap-trapper. cDNA went through one round of normalization to Rot = 10.0 and subtraction to Rot = 100.0. Second strand cDNA was prepared with the primer adapter of sequence [5' GAGAGAGAGATTCTCGAGTTAATTAAATTAATCCCCCCCCCCCCC 3']. cDNA was cloned into the XhoI and BamHI sites. Vector: a modified pBluescript KS(+) after bulk excision from Lambda FLC I. Cloning sites, 5' end: SalI; 3' end: BamHI

text: 6 days neonate

text: Mus musculus

text: SalI

text: BamHI

text: mixed

text: head

text: RIKEN full-length enriched, 6 days neonate head


text: live

text: Generic

text: cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and Genome Science Laboratory in RIKEN. Division of Experimental Animal Research in Riken contributed to prepare mouse tissues. 1st strand cDNA was primed with a primer [5' GAGAGAGAGAAGGATCCAAGAGCTCTTTTTTTTTTTTTTTTVN 3'], cDNA was prepared by using trehalose thermo-activated reverse transcriptase and subsequently enriched for full-length by cap-trapper. cDNA went through one round of normalization to Rot = 10.0 and subtraction to Rot = 100.0. Second strand cDNA was prepared with the primer adapter of sequence [5' GAGAGAGAGATTCTCGAGTTAATTAAATTAATCCCCCCCCCCCCC 3']. cDNA was cloned into the XhoI and BamHI sites. Vector: a modified pBluescript KS(+) after bulk excision from Lambda FLC I. Cloning sites, 5' end: SalI; 3' end: BamHI.

text: 10 days neonate

text: Mus musculus

text: SalI

text: BamHI

text: mixed

text: head

text: RIKEN full-length enriched, 10 days neonate head


text: live

text: Generic

text: cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and Genome Science Laboratory in RIKEN. Division of Experimental Animal Research in Riken contributed to prepare mouse tissues. 1st strand cDNA was primed with a primer [5' GAGAGAGAGAAGGATCCAAGAGCTCTTTTTTTTTTTTTTTTVN 3'], cDNA was prepared by using trehalose thermo-activated reverse transcriptase and subsequently enriched for full-length by cap-trapper. cDNA went through one round of subtraction to Rot = 100.0 Second strand cDNA was prepared with the primer adapter of sequence [5' GAGAGAGAGATTCTCGAGTTAATTAAATTAATCCCCCCCCCCCCC 3']. cDNA was cloned into the XhoI and BamHI sites. Vector: a modified pBluescript KS(+) after bulk excision from Lambda FLC I. Cloning sites, 5' end: SalI; 3' end: BamHI

text: 6 days embryo

text: Mus musculus

text: SalI

text: BamHI

text: mixed

text: RIKEN full-length enriched, 6 days embryo


text: live

text: Generic

text: 8 days embryo

text: Mus musculus

text: SalI

text: BamHI

text: mixed

text: RIKEN full-length enriched, 8 days embryo


text: Washington University School of Medicine

text: live

text: Generic

characteristic: cultivar

text: This cDNA library was constructed from mRNA isolated from seed coats (100-200mgs) of greenhouse grown plants. The library was prepared using the Life Technologies pSuperScript cDNA library construction kit. Complimentary DNA was synthesized from mRNA using a poly (dT) sequence with a Not I restrictions site. Sal I linkers adapers were ligated to the blunt-ended cDNA fragments followed by Not I digestion. The cDNA fragments were directionally cloned into the Not I-Sal I restriction site of the pSPORT1 vector. The ligated cDNA fragments were transformed into E.coli Electromax DH10B host cells. This library was constructed by Dr. Lila Vodkin and Dr. Anu Khanna.

text: Glycine max

text: SalI

text: NotI

text: seed coats of greenhouse grown plants

text: plasmid

characteristic: vector


text: Lawrence Livermore National Laboratory

text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Library constructed by Life Technologies. Investigator providing samples: Gilbert Smith, NIH

text: 3 months, virgin

characteristic: dev-stage

text: mammary

characteristic: organ

text: Mus musculus

text: SalI

text: NotI

text: FVB/N

text: tumor, biopsy sample

characteristic: title

text: phagemid (ampicillin resistant)


characteristic: External Id

text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Library constructed by Life Technologies. Investigator providing samples: Jeffrey Green, M.D., NIH

text: 5 months

text: mammary

text: Mus musculus

characteristic: organism

text: SalI

text: NotI

text: female, virgin

text: FVB/N

characteristic: strain

text: infiltrating ductal carcinoma

text: phagemid (ampicillin resistant)


text: The Institute of Physical and Chemical Research (RIKEN)

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: adult

text: Mus musculus

text: SalI

text: BamHI

text: male

text: medulla oblongata

characteristic: tissue-type

text: RIKEN full-length enriched, adult male medulla oblongata


characteristic: INSDC last update

text: live

text: Generic

text: adult

characteristic: lab-host

text: Mus musculus

text: SalI

text: BamHI

text: male

text: olfactory brain

text: RIKEN full-length enriched, adult male olfactory bulb


text: University of Edinburgh

text: live

characteristic: INSDC status

text: Generic

text: Ascaris suum is an intestinal nematode parasite of pigs. The library was constructed from dissected nerve cord and associated muscle tissue for Dr. T. Geary, Pharmacia-Upjohn Inc, Kalamazoo, MI, USA [tggeary@am.pnu.com]

text: adult

text: Ascaris suum

text: EcoRI (5'end)

text: EcoRI (3'end)

text: mixed

characteristic: sex

text: nerve cord and associated muscle

text: Ascaris suum (parasitic nematode) adult nerve cord and muscle

text: phage

text: Lambda Zap II


text: University of Edinburgh

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Ascaris suum is an intestinal nematode parasite of pigs. The library was constructed from dissected body wall muscle and hypodermis tissue for Dr. T. Geary, Pharmacia-Upjohn Inc, Kalamazoo, MI, USA [tggeary@am.pnu.com]

text: adult

text: Ascaris suum

text: NotI (5'end)

text: NotI (3'end)

text: mixed

text: body wall muscle and hypodermis

text: Ascaris suum (parasitic nematode) body wall muscle and hypodermis

text: phage

text: Lambda Zap II


text: live

text: Generic

text: cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and Genome Science Laboratory in RIKEN. Division of Experimental Animal Research in Riken contributed to prepare mouse tissues. 1st strand cDNA was primed with a primer [5' GAGAGAGAGAAGGATCCAAGAGCTCTTTTTTTTTTTTTTTTVN 3'], cDNA was prepared by using trehalose thermo-activated reverse transcriptase and subsequently enriched for full-length by cap-trapper. cDNA went through one round of normalization to Rot = 10.0 and subtraction to Rot = 100.0. Second strand cDNA was prepared with the primer adapter of sequence [5' GAGAGAGAGATTCTCGAGTTAATTAAATTAATCCCCCCCCCCCCC 3']. cDNA was cloned into the XhoI and BamHI sites. Vector: a modified pBluescript KS(+) after bulk excision from Lambda FLC I. Cloning sites, 5' end: SalI; 3' end: BamHI

characteristic: description

text: 10 days neonate

text: Mus musculus

text: SalI

text: BamHI

text: mixed

text: cerebellum

text: RIKEN full-length enriched, 10 days neonate cerebellum


text: live

text: Generic

text: cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and Genome Science Laboratory in RIKEN. Division of Experimental Animal Research in Riken contributed to prepare mouse tissues. 1st strand cDNA was primed with a primer [5' GAGAGAGAGAAGGATCCAAGAGCTCTTTTTTTTTTTTTTTTVN 3'], cDNA was prepared by using trehalose thermo-activated reverse transcriptase and subsequently enriched for full-length by cap-trapper. Second strand cDNA was prepared with the primer adapter of sequence [5' GAGAGAGAGATTCTCGAGTTAATTAAATTAATCCCCCCCCCCCCC 3']. cDNA was cloned into the XhoI and BamHI sites. Vector: a modified pBluescript KS(+) after bulk excision from Lambda FLC I. Cloning sites, 5' end: SalI; 3' end: BamHI. In 12 days embryo, ovary and testis may partially contaminate the other sex.

text: 12 days embryo

text: Mus musculus

text: SalI

text: BamHI

text: female

text: ovary

text: RIKEN full-length enriched, 12 days embryo female ovary


text: live

text: Generic

text: cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and Genome Science Laboratory in RIKEN. Division of Experimental Animal Research in Riken contributed to prepare mouse tissues. 1st strand cDNA was primed with a primer [5' GAGAGAGAGAAGGATCCAAGAGCTCTTTTTTTTTTTTTTTTVN 3'], cDNA was prepared by using trehalose thermo-activated reverse transcriptase and subsequently enriched for full-length by cap-trapper. Second strand cDNA was prepared with the primer adapter of sequence [5' GAGAGAGAGATTCTCGAGTTAATTAAATTAATCCCCCCCCCCCCC 3']. cDNA was cloned into the XhoI and BamHI sites. Vector: a modified pBluescript KS(+) after bulk excision from Lambda FLC I. Cloning sites, 5' end: SalI; 3' end: BamHI

text: 12 days embryo

text: Mus musculus

text: SalI

text: BamHI

text: male

text: wolffian duct includes surrounding region

text: RIKEN full-length enriched, 12 days embryo male wolffian duct


text: live

text: Generic

text: 12 days embryo

text: Mus musculus

text: SalI

text: BamHI

text: female

text: mullerian duct includes surrounding region

text: RIKEN full-length enriched, 12 days embryo female mullerian duct


text: live

text: Generic

text: cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and Genome Science Laboratory in RIKEN. Division of Experimental Animal Research in Riken contributed to prepare mouse tissues. 1st strand cDNA was primed with a primer [5' GAGAGAGAGAAGGATCCAAGAGCTCTTTTTTTTTTTTTTTTVN 3'], cDNA was prepared by using trehalose thermo-activated reverse transcriptase and subsequently enriched for full-length by cap-trapper. cDNA went through one round of subtraction to Rot = 93.0 Second strand cDNA was prepared with the primer adapter of sequence [5' GAGAGAGAGATTCTCGAGTTAATTAAATTAATCCCCCCCCCCCCC 3']. cDNA was cloned into the XhoI and BamHI sites. Vector: a modified pBluescript KS(+) after bulk excision from Lambda FLC I. Cloning sites, 5' end: SalI; 3' end: BamHI

text: 11 days embryo

text: Mus musculus

text: SalI

text: BamHI

text: mixed

text: gonad

text: RIKEN full-length enriched, 11 days embryo gonad


text: The Institute of Physical and Chemical Research (RIKEN)

text: live

text: Generic

text: cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and Genome Science Laboratory in RIKEN. Division of Experimental Animal Research in Riken contributed to prepare mouse tissues. 1st strand cDNA was primed with a primer [5' GAGAGAGAGAAGGATCCAAGAGCTCTTTTTTTTTTTTTTTTVN 3'], cDNA was prepared by using trehalose thermo-activated reverse transcriptase and subsequently enriched for full-length by cap-trapper. Second strand cDNA was prepared with the primer adapter of sequence [5' GAGAGAGAGATTCTCGAGTTAATTAAATTAATCCCCCCCCCCCCC 3']. cDNA was cleaved with XhoI and BamHI. Vector: a modified pBluescript KS(+) after bulk excision from Lambda FLC I. Cloning sites, 5' end: SalI; 3' end: BamHI. Host: DH10B. ~RNA was provided by Akira Nakagawara, Div. of Biochemistry, Chiba Cancer Center Research Institute, 666-2 Nitona, Chuoh-ku, Chiba, 260-8717 Japan, whose assistance we gratefully acknowledge.

text: 2 days neonate

text: Mus musculus

text: SalI

text: BamHI

text: mixed

text: sympathetic ganglion

text: RIKEN full-length enriched, 2 days neonate sympathetic ganglion


text: live

text: Generic

text: cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and Genome Science Laboratory in RIKEN. Division of Experimental Animal Research in Riken contributed to prepare mouse tissues. 1st strand cDNA was primed with a primer [5' GAGAGAGAGAAGGATCCAAGAGCTCTTTTTTTTTTTTTTTTVN 3'], cDNA was prepared by using trehalose thermo-activated reverse transcriptase and subsequently enriched for full-length by cap-trapper. cDNA went through one round of normalization to Rot = 10.0 and subtraction to Rot = 185.0. Second strand cDNA was prepared with the primer adapter of sequence [5' GAGAGAGAGATTCTCGAGTTAATTAAATTAATCCCCCCCCCCCCC 3']. cDNA was cloned into the XhoI and BamHI sites. Vector: a modified pBluescript KS(+) after bulk excision from Lambda FLC I. Cloning sites, 5' end: SalI; 3' end: BamHI

text: 1 day pregnant, adult

text: Mus musculus

text: SalI

text: BamHI

text: female

text: ovary

text: RIKEN full-length enriched, 1 day pregnant adult female ovary


text: live

text: Generic

text: adult

text: Mus musculus

text: SalI

text: BamHI

text: male

text: adrenal gland

text: RIKEN full-length enriched, adult male adrenal gland


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCGGGCCTTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library is normalized; constructed by Bento Soares and M.Fatima Bonaldo.

text: DH10B (phage-resistant

text: cervix

text: Homo sapiens

text: Not I

text: Eco RI

text: phagemid (ampicillin resistant)

characteristic: vector


text: live

text: Generic

characteristic: cell-line

text: Library prepared by Edge BioSystems.

text: Blood

text: Homo sapiens

text: T cell leukemia

text: plasmid (chloramphenicol resistant, grown at 30C)


text: live

text: Generic

text: Library prepared by Edge BioSystems.

text: Lymph

characteristic: organ

text: Homo sapiens

text: Burkitt lymphoma


text: live

text: Generic

text: cDNA made by oligo-dT priming. Directionally cloned into CeuI/SceI sites using the following 5' adaptor: taactataacggtcctaaggtagcga and 3' adaptor: tttcattacctctttctccgcaccccacataaa. Average insert size 900 bp. Library prepared by Edge BioSystems.

text: DH10B (phage-resistant)

text: Cervix

text: Homo sapiens

text: SceI

text: CeuI

characteristic: title


characteristic: External Id

text: live

text: Generic

text: cDNA made by oligo-dT priming. Directionally cloned into EcoRI/XhoI sites using the following 5' adaptor: GGCACGAG(G). Size-selected >500bp for average insert size 1.8kb. Library constructed by Ling Hong in the laboratory of Gerald M. Rubin (University of California, Berkeley) using ZAP-cDNA synthesis kit (Stratagene) and Superscript II RT (Life Technologies).

text: DH10B (phage-resistant)

text: lung

text: Homo sapiens

text: XhoI

text: EcoRI

text: small cell carcinoma


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.5 kb. Library prepared by Life Technologies.

text: cervix

text: Homo sapiens

characteristic: organism

text: NotI

text: SalI

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCCGAAGTTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library is normalized; constructed by Bento Soares and M.Fatima Bonaldo.

text: DH10B (phage-resistant)

text: cervix

text: Homo sapiens

text: Not I

text: Eco RI

text: tumor

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and Genome Science Laboratory in RIKEN. Division of Experimental Animal Research in Riken contributed to prepare mouse tissues. 1st strand cDNA was primed with a primer [5' GAGAGAGAGAAGGATCCAAGAGCTCTTTTTTTTTTTTTTTTVN 3'], cDNA was prepared by using trehalose thermo-activated reverse transcriptase and subsequently enriched for full-length by cap-trapper. Second strand cDNA was prepared with the primer adapter of sequence [5' GAGAGAGAGATTCTCGAGTTAATTAAATTAATCCCCCCCCCCCCC 3']. cDNA was cloned into the XhoI and BamHI sites. Vector: a modified pBluescript KS(+) after bulk excision from Lambda FLC I. Cloning sites, 5' end: SalI; 3' end: BamHI

text: egg

characteristic: dev-stage

text: Mus musculus

text: SalI

text: BamHI

text: female

characteristic: strain

text: in vitro fertilized eggs

text: RIKEN full-length enriched, in vitro fertilized eggs


characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: adult

text: Mus musculus

text: SalI

text: BamHI

text: male

text: eyeball

text: RIKEN full-length enriched, adult male eyeball


characteristic: INSDC last update

text: live

text: Generic

text: adult

text: Mus musculus

text: SalI

text: BamHI

text: male

text: corpus striatum

characteristic: tissue-type

text: RIKEN full-length enriched, adult male corpus striatum


text: The Institute of Physical and Chemical Research (RIKEN)

text: live

characteristic: INSDC status

text: Generic

text: adult

characteristic: lab-host

text: Mus musculus

text: SalI

text: BamHI

text: male

text: cortex

text: RIKEN full-length enriched, adult male cortex


text: live

text: Generic

characteristic: NCBI submission model

text: cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and Genome Science Laboratory in RIKEN. Division of Experimental Animal Research in Riken contributed to prepare mouse tissues. 1st strand cDNA was primed with a primer [5' GAGAGAGAGAAGGATCCAAGAGCTCTTTTTTTTTTTTTTTTVN 3'], cDNA was prepared by using trehalose thermo-activated reverse transcriptase and subsequently enriched for full-length by cap-trapper. cDNA went through one round of subtraction to Rot = 185.0 Second strand cDNA was prepared with the primer adapter of sequence [5' GAGAGAGAGATTCTCGAGTTAATTAAATTAATCCCCCCCCCCCCC 3']. cDNA was cloned into the XhoI and BamHI sites. Vector: a modified pBluescript KS(+) after bulk excision from Lambda FLC I. Cloning sites, 5' end: SalI; 3' end: BamHI

text: 11 days embryo

text: Mus musculus

text: SalI

text: BamHI

text: mixed

characteristic: sex

text: wolffian duct and mullerian duct

text: RIKEN full-length enriched, 11 days embryo wolffian and mullerian ducts


text: live

text: Generic

characteristic: NCBI submission package

characteristic: description

text: 15 days embryo

text: Mus musculus

text: SalI

text: BamHI

text: male

text: testis

text: RIKEN full-length enriched, 15 days embryo male testis


text: live

text: Generic

text: cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and Genome Science Laboratory in RIKEN. Division of Experimental Animal Research in Riken contributed to prepare mouse tissues. 1st strand cDNA was primed with a primer [5' GAGAGAGAGAAGGATCCAAGAGCTCTTTTTTTTTTTTTTTTVN 3'], cDNA was prepared by using trehalose thermo-activated reverse transcriptase and subsequently enriched for full-length by cap-trapper. cDNA went through one round of normalization to Rot = 10.0 and subtraction to Rot = 185.0. Second strand cDNA was prepared with the primer adapter of sequence [5' GAGAGAGAGATTCTCGAGTTAATTAAATTAATCCCCCCCCCCCCC 3']. cDNA was cloned into the XhoI and BamHI sites. Vector: a modified pBluescript KS(+) after bulk excision from Lambda FLC I. Cloning sites, 5' end: SalI; 3' end: BamHI

text: 15 days embryo

text: Mus musculus

text: SalI

text: BamHI

text: mixed

text: RIKEN full-length enriched, 15 days embryonic body between diaphragm and neck


text: live

text: Generic

text: 15 days embryo

text: Mus musculus

text: SalI

text: BamHI

text: mixed

text: embryonic body below diaphragm

text: RIKEN full-length enriched, 15 days embryonic body below diaphragm


text: live

text: Generic

text: adult

text: Mus musculus

text: SalI

text: BamHI

text: female

text: uterus

text: RIKEN full-length enriched, adult female uterus


text: live

text: Generic

text: 16 days embryo

text: Mus musculus

text: SalI

text: BamHI

text: mixed

text: lung

text: RIKEN full-length enriched, 16 days embryo lung


text: live

text: Generic

text: 11 days embryo

text: Mus musculus

text: SalI

text: BamHI

text: mixed

text: RIKEN full-length enriched, 11 days embryonic body between diaphragm region and neck


text: live

text: Generic

text: 14 days embryo

text: Mus musculus

text: SalI

text: BamHI

text: female

text: mammary gland

text: RIKEN full-length enriched, 14 days embryo female mammary gland


text: live

text: Generic

text: adult

text: Mus musculus

text: SalI

text: BamHI

text: male

text: colon

text: RIKEN full-length enriched, adult male colon


text: live

text: Generic

text: adult

text: Mus musculus

text: SalI

text: BamHI

text: male

text: cecum

text: RIKEN full-length enriched, adult male cecum


text: live

text: Generic

text: Anchor enzyme: NlaIII~ This SAGE library is from Tumor type: Breast, ductal carcinoma in situ. 'Velculescu, V. E., Zhang, L., Vogelstein, B., and Kinzler, K. W. (1995) Serial Analysis of Gene Expression. Science 270, 484-487'

text: Homo sapiens

text: Breast, ductal carcinoma in situ.


text: live

text: Generic

text: Anchor enzyme: NlaIII~ This SAGE library is from Tumor type: Brain, normal cerebellum. 'Velculescu, V. E., Zhang, L., Vogelstein, B., and Kinzler, K. W. (1995) Serial Analysis of Gene Expression. Science 270, 484-487'

text: Brain

text: Homo sapiens

text: normal cerebellum


text: Washington University Medical School

text: live

text: Generic

text: Brugia malayi

text: McCall

text: Brugia malayi McCall


text: live

text: Generic

text: adult

text: Mus musculus

text: SalI

text: BamHI

text: male

text: epididymis

text: RIKEN full-length enriched, adult male epididymis


text: The Institute of Physical and Chemical Research (RIKEN)

text: live

text: Generic

text: adult

text: Mus musculus

text: SalI

text: BamHI

text: male

text: diencephalon

text: RIKEN full-length enriched, adult male diencephalon


text: Miyazaki University

text: live

text: Generic

text: common name:rainbow trout ; infected by infectious hematopoietic necrosis virus

text: Oncorhynchus mykiss

text: kidney

text: Oncorhynchus mykiss Kidney infected by infectious hematopoietic necrosis virus


text: Miyazaki University

text: live

text: Generic

text: Oncorhynchus mykiss

text: Oncorhynchus mykiss Gill infected by infectious hematopoietic necrosis virus


text: University of Maryland

text: live

text: Generic

text: Metarhizium anisopliae was grown on insect cuticle for 24 hours. A cDNA library was constructed in the unidirectional Lambda vector, UniZap

text: Metarhizium anisopliae

text: ARSEF 2575

text: Metarhizium anisopliae ARSEF 2575

text: UniZap

characteristic: vector


text: Kyushu University

text: live

text: Generic

text: peritoneal cells

characteristic: cell type

text: Cyprinus carpio

text: Cyprinus carpio peritoneal cells


text: Ludwig Institute for Cancer Research

text: live

text: Generic

text: A mini-library was made by cloning products derived from ORESTES PCR (U.S. Letters Patent application No. 196,716 - Ludwig Institute for Cancer Research) profiles into the pUC 18 vector. Reverse transcription of tissue mRNA and cDNA amplification were performed under low stringency conditions.

text: Adult

text: breast

characteristic: organ

text: Homo sapiens

text: SmaI

text: SmaI

characteristic: title

text: Plasmid


characteristic: External Id

text: Ludwig Institute for Cancer Research

text: live

text: Generic

text: Adult

text: colon

text: Homo sapiens

characteristic: organism

text: SmaI

text: SmaI

text: Plasmid


text: Ludwig Institute for Cancer Research

text: live

text: Generic

text: Adult

characteristic: dev-stage

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: Ludwig Institute for Cancer Research

characteristic: INSDC center name

text: live

text: Generic

text: Adult

text: stomach

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: Smith College Department of Biological Sciences

characteristic: INSDC first public

text: live

text: Generic

text: Lymphatic filarial nematode parasite of humans. mRNA was prepared from young adult worms isolated from the peritoneal cavity of jirds on day 25 after infection and converted to double-stranded cDNA using reverse transcriptase and oligo(dT) followed by RNase H and DNA pol I. The library has 6.2 x 105 independent recombinants and the average insert size is approx.1101bp. The library was constructed by Michelle Lizotte-Waniewski. The library is available from Dr. S.A. Williams, email: genome@neal.smith.edu.

text: young adult, twenty five days after infection

text: XL1-Blue MRF'

text: Brugia malayi

text: Eco RI

text: Xho I

text: Brugia malayi young adult day 25 cDNA (SAW99MLW-BmYD25)

text: Phage

text: Lambda Uni-ZAP XR


characteristic: INSDC last update

text: live

text: Generic

text: Anchor enzyme: NlaIII~ This SAGE library is from Tumor type: Ovary, primary ovarian tumour, serous adenocarcinoma. 'Velculescu, V. E., Zhang, L., Vogelstein, B., and Kinzler, K. W. (1995) Serial Analysis of Gene Expression. Science 270, 484-487'

text: Ovary

text: Homo sapiens

text: Primary ovarian tumor, serous adenocarcinoma


text: The General Environmental Technos Co., Ltd

text: live

text: Generic

text: The cDNA clone was isolated from the halotolerant green alga Chlamydomonas HS-5 by a functional expression screening in E.coli cells. The principle of the screening method was based on the acquisition of stress tolerance of the bacterial cells carrying the cDNA.

text: Chlamydomonas sp. HS-5

characteristic: strain

text: Chlamydomonas sp. HS-5 lambda ZAP II

text: lambda ZAP II


text: Washington University School of Medicine

text: live

characteristic: INSDC status

text: Generic

text: Oligo (dT) primed library from L3 worms isolated from dog. cDNA was contructed and cloned unidirectionally into the vector from the EcoRI to the XhoI site. The library went through one round of amplification.

text: 3rd stage larva (L3)

text: E. coli, XL-1 Blue MRF' (Stratagene)

text: Ancylostoma caninum

text: EcoRI (5')

text: XhoI (3')

text: female and male

text: Shanghai strain

text: Hawdon Ancylostoma caninum L3

text: Phagemid (Ampicillin resistant)

text: Lambda Uni-ZAP XR Vector (Stratagene)


text: live

text: Generic

text: Williams

characteristic: cultivar

text: This cDNA library was constructed from mRNA isolated from cotyledons of 3- and 7-day-old Williams seedlings which were propagated on paper towels with distilled water. The cotyledons were flash-frozen in liquid nitrogen, then lyophilized for 72 hours. Unequal amounts of mRNA was used for cDNA synthesis. Stratagene's cDNA Synthexix Kit (catalog number 200401) was used to synthesize the cDNA. First- stranded synthesis was performed with 5-methyl dCTP, hence the ligated cDNA was hemimethylated. A modification of Stratagene's first-strand synthesis primer was used. An anchor nucleotide (V=A, C, or G) was added to the 3' end of the primer [GAGAGAGAGAGAGAGAGAGAACTAGTCTCGAG(T)18] to anchor the primer at the 5' end of the poly(A) tract. After second- strand synthesis, the cDNA ends were filled in with cloned Pfu DNA, ligated to EcoRI adapters and subsequently phosphorylated. The XhoI site within the first-strand synthesis primer was then restricted by digestion with XhoI; all XhoI sites in the cDNA would be protected by their hemimethylated status. The cDNA constructs were size-fractionated with a 500 bp cutoff, using GibcoBRL Life Technologies' cDNA Size Fractionation column. The column eluent was then ligated into Stratagene's pBluescript(tm) II XR Predigested vector (pBluescript II SK(+) that has been digested with EcoRI and XhoI, and phosphorylated by Stratagene). 97% of the white and blue colonies appear to contain recombinant plasmids with cDNA inserts, based on size (n=30). This library was constructed by Dr. Paul Keim and Dr. Virginia Coryell.

characteristic: lab-host

text: Glycine max

text: EcoRI

text: XhoI

characteristic: tissue-type

text: plasmid

text: pBluescript II SK+


text: Anhui Medical University

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: adult worms

text: Schistosoma japonicum

text: mixed

characteristic: sex

text: Chinese strain

text: adult worms of Schistosoma japonicum


text: The Neurosciences Institute

text: live

text: Generic

text: Mus musculus

text: FVB

text: muscle

text: Mus musculus muscle FVB


text: Graduate School of Agriculture Kyoto University

text: live

text: Generic

text: Japanese Black

characteristic: description

text: adult

text: Bos taurus

text: male

text: adipose

text: adipose tissue library


text: The University of Melbourne - School of Forestry

text: live

text: Generic

text: 'Mature leaf' cDNAs (Driver) were subtracted from cambial cDNAs (Tester) using CLONTECH PCR-Select cDNA subtraction technology. Cambial tissue was collected at 40% stem hight. Bark was removed and scrapings were taken from the exposed xylogenic tissue and the inside of the bark ensuring representation of cambial initials while avoiding contamination with phloem tissue.

text: Eucalyptus globulus

text: Cambial

text: Eucalypt cambium-minus-leaf subtracted cDNAs


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: germinal center B cells

characteristic: cell type

characteristic: cell-line

text: Constructed from size fractionated cytoplasmic mRNA (0.5-1.5kb). Directionally cloned. Cells provided by Louis M. Staudt, Ph.D. Library preparation by Maria de Fatima Bonaldo, Ph.D. and M. Bento Soares, Ph.D.

text: DH10B (LTI)

text: Homo sapiens

text: NotI

text: Eco RI

text: lymph

text: plasmid

characteristic: vector


characteristic: External Id

text: live

text: Generic

text: germinal center B cells

text: Constructed from size fractionated cytoplasmic mRNA (1.5-2.5kb). Directionally cloned. Cells provided by Louis M. Staudt, Ph.D. Library preparation by Maria de Fatima Bonaldo, Ph.D. and M. Bento Soares, Ph.D.

text: DH10B (LTI)

text: Homo sapiens

characteristic: organism

text: NotI

text: Eco RI

text: lymph

characteristic: title

text: plasmid


characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: germinal center B cells

text: Constructed from size fractionated cytoplasmic mRNA (2.5-3.5kb). Directionally cloned. Cells provided by Louis M. Staudt, Ph.D. Library preparation by Maria de Fatima Bonaldo, Ph.D. and M. Bento Soares, Ph.D.

text: DH10B (LTI)

text: Homo sapiens

text: NotI

text: Eco RI

text: lymph

text: plasmid


characteristic: INSDC last update

text: live

text: Generic

text: Total RNA was prepared from human islet cells and from exocrine pancreatic tissues. These two samples were then used as template material for PCR using an anchored T12VG primer and random hexamers for differential display. Islet specific differential display bands were isolated, subcloned and sequenced using vector primers.

text: adult

characteristic: dev-stage

text: pancreas

characteristic: organ

text: Homo sapiens

text: pancreatic islets

text: human islet cDNA differential display

text: plasmid


text: Changhai Hospital

text: live

characteristic: INSDC status

text: Generic

text: Lymphocyte and monocyte

text: 4hrs after female F344 rats were scalded by hot water, lymphocytes and monocytes were extracted from blood and compared to rats without scald.

text: Rattus norvegicus

text: Female

characteristic: strain

text: Blood

text: Subtracted products from lymphocytes and monocytes of rattus


text: live

text: Generic

characteristic: NCBI submission model

text: exon-trapped clone isolated using the procedure of Buckler et al. PNAS (1991) 88:4005-4009

text: Homo sapiens

text: Human chromosome 17q21 exon-trapped


text: Max-Planck-Institute for Chemical Ecology

text: live

text: Generic

characteristic: NCBI submission package

text: Hydroponically grown plants of the inbred line DI92 of Nicotiana attenuata were exposed to herbivory by first-instar larvae of the tobacco hornworm (Manduca sexta, Lepidoptera, Sphingidae) for 24 hrs. Total cellular RNAs were extracted from rosette leaves and served as templates for mRNA differential display RT-PCR. The cDNAs of insect-responsive genes were cloned into the pCR2.1-TOPO vector (Invitrogen) and subjected to sequencing.

text: Vegetative stage

text: Escherichia coli TOPO 10F' (Invitrogen)

characteristic: lab-host

text: Nicotiana attenuata

text: Eco RI

text: Eco RI

text: Inbred Line Utah DI92

text: Leaves

characteristic: tissue-type

text: cDNAs of Nicotiana attenuata insect-responsive genes isolated by mRNA differential-display RT-PCR

text: Plasmid

text: pCR2.1-TOPO (Invitrogen)


text: Univ. Salamanca

text: live

text: Generic

text: Castellana

characteristic: cultivar

text: 5 days old seedlings

text: Cicer arietinum

text: etiolated epicotyls

text: Cicer arietinum etiolated epicotyls 5 days old seedlings


text: Institute of Basic Medical Sciences

text: live

text: Generic

text: Rattus norvegicus

text: Rattus norvegicus library (Ma MZ)


text: North Carolina State University

text: live

text: Generic

text: preparasitic juvenile stage 2

text: Heterodera glycines

text: Heterodera glycines OP50 preparasitic juvenile stage 2


text: University of Delaware

text: live

text: Generic

text: E.coli XL1 Blue

text: Gallus gallus

text: Male

characteristic: sex

text: liver

text: chicken liver cDNA library

text: pBluescript II KS


text: Washington University School of Medicine

text: live

text: Generic

text: Supernod

text: The mRNA was isolated from roots of Glycine max 'Supernod' plants generously donated by Dr. Gary Stacey. The seedlings were innoculated with Bradyrhizobium japonicus, strain USDA110 priot to harvest. Stratagene's cDNA synthesis Kit (catalog number 200401) was used to synthesize the cDNA. First-strand synthesis was performed with 5-methyl dCTP, hence the ligated cDNA was hemimethylated. A modification of Stratagene's first-strand synthesis primer was used. An 'anchor' nucleotide (V=A,C, or G) was added to the 3' end of the primer [GAGAGAGAGAGAGAGAGAGAACTAGTCTCGAG(T)18V] to anchor the primer at the 5' end of the poly(A) tract. After second-strand synthesis, the cDNA ends were filled in with cloned Pfu DNA polymerase, ligated to EcoRI adapters and subsequently phosphorylated. The XhoI site within the first-strand synthesis primer was then restricted by digestion with XhoI; all XhoI sites in the cDNA would be protected by their hemimethylated status. The cDNA constructs were size-fractionated with a 500bp cutoff, using GibcoBRL Life Technologies' cDNA Size Fractionation column. The column eluent was then ligated into Stratagene's pBluescript II XR Predigested vector (pBluescript II SK(+) that has been digested with EcoRI and XhoI, and phosporylated by Stratagene). Both the white and blue colonies appear to contain recombinant plasmids with cDNA inserts, based on size (n=25). This library was constructed by Dr. Paul Keim and Dr. Virginia Coryell.

characteristic: description

text: Glycine max

text: EcoRI

text: XhoI

text: roots of 'Supernod' plants

text: plasmid

text: pBluescript II XR

characteristic: vector


characteristic: External Id

text: live

text: Generic

text: The NCI_CGAP_Sub4 library is a subtracted library derived from the NCI_CGAP_Sub2 library which is a subtracted library derived from the NCI_CGAP_Sub1 library, which is a subtracted library derived from BI. BI constitutes a mixture of 21 normalized or subtracted NCI_CGAP libraries: NCI_CGAP_Co4, NCI_CGAP_Pr22, NCI_CGAP_Pr28, NCI_CGAP_Co10, NCI_CGAP_Co16, NCI_CGAP_Kid5, NCI_CGAP_Kid12, NCI_CGAP_Kid3, NCI_CGAP_Kid11, NCI_CGAP_Lym2, NCI_CGAP_Br2, NCI_CGAP_Co8, NCI_CGAP_CLL1, NCI_CGAP_Lei2, NCI_CGAP_Brn23, NCI_CGAP_Lu5, NCI_CGAP_Lu24, NCI_CGAP_Lu19, NCI_CGAP_GC4, NCI_CGAP_GC6, NCI_CGAP_Brn25. These 21 libraries were pooled and a single-stranded DNA preparation of the resulting mixture was used as a tracer in a subtractive hybridization with a driver whose composition is detailed below: NCI_CGAP_Kid3 pool 1 : LLAM 3334-3337, 3682-3683, 3798-3803 (IMAGE CloneIDs 1322376-1323911, 1456008-1456775, 1500552-1502855) NCI_CGAP_Kid5 pool 1 : LLAM 3338-3342, 3722-3725, 3776-3778 (IMAGE CloneIDs 1323912-1325831, 1471368-1472903, 1492104-1493255) NCI_CGAP_Lu5 pool 1 : LLAM 3575-3582, 3851-3854 (IMAGE CloneIDs 1414920-1417991, 1520904-1522439) NCI_CGAP_GC4 pool 1 : LLAM 3164-3167, 3716-3720, 3733-3735 (IMAGE CloneIDs 1257096-1258631, 1469064-1470983, 1475592-1476743) NCI_CGAP_Pr22 pool 1 : LLAM 2457-2459, 2758-2759, 3062-3068 (IMAGE CloneIDs 985608-986759, 1101192-1101959, 1217928-1220615) NCI_CGAP_Co10 pool 1 : LLAM 2644-2653, 2871-2872 (IMAGE CloneIDs 1057416-1061255, 1144584-1145351) Subtraction was performed as previously described [Bonaldo, Lennon & Soares (1996): Normalization and Subtraction: Two Approaches To Facilitate Gene Discovery. Genome Research 6, 791-806.]

text: DH10B (Life Technologies)

text: Homo sapiens

characteristic: organism

text: Not I

text: Eco RI

text: Phagemid


text: University of Georgia

characteristic: INSDC center name

text: live

text: Generic

text: genotype A17

text: cDNA was prepared from polyA+ enriched RNA from roots harvested at 10 days post inoculation with Phytophthora medicaginis. The cDNA was directionally ligated into the Uni-ZAP XR vector from Stratagene and packaged using Gigapack III Gold packaging extracts. Plasmids containing cDNA inserts were excised from the recombinant lambda-ZAP phage using Ex-Assist helper phage and propagated in XLOLR cells. Note: EST may be of fungal origin.

text: E. coli strain XLOLR

text: Medicago truncatula

text: EcoRI

text: XhoI

text: roots infected with Phytophthora medicaginis

text: DSIR

characteristic: title

text: Plasmid

text: pBluescript SK-


text: University of Minnesota

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: genotype A17

text: cDNA was prepared from polyA+ enriched RNA from the mixture of effective nodules of 40 day old plants harvested 36 hours post shoot removal and nodules collected from 2 month old plants at mid-pod stage. The cDNA was directionally ligated into the Uni-ZAP XR vector from Stratagene and packaged using Gigapack III Gold packaging extracts. Plasmids containing cDNA inserts were excised from the recombinant lambda-ZAP phage using Ex-Assist helper phage and propagated in SOLR cells.

text: mixture of effective nodules from 40 day old plants harvested 36 hours post shoot removal and nodules collected from 2 month old plants at mid-pod stage

text: E. coli strain SOLR

text: Medicago truncatula

text: EcoRI

text: XhoI

text: senescent root nodules

text: GVSN

text: Plasmid

text: pBluescript SK +/-


text: Institute of Medical Science, Univ. of Tokyo

text: live

text: Generic

text: Stratagene cat. #937314

text: whole brain

characteristic: organ

text: Mus musculus

text: female

text: Mouse Brain cDNA Library (cat. #937314)

text: Phage

text: Uni-ZAP XR vector


text: Iwate Biotechnology Research Center

text: live

characteristic: INSDC status

text: Generic

text: Nicotiana benthamiana

text: leaf

text: Nicotiana benthamiana leaf


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Library constructed by Life Technologies. Investigators providing samples: Lothar Hennighausen/Chu-Xia Deng, NIH Reference for transgenic model: Xu et al., Nature Genetics 22, 37-43 (1999).

text: 10 months

characteristic: dev-stage

text: mammary

text: Mus musculus

text: SalI

text: NotI

characteristic: strain

text: tumor, gross tissue

text: phagemid (ampicillin resistant)


text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Cloned unidirectionally. Primer: Oligo dT. Library constructed by Life Technologies. Investigator providing samples: Gilbert Smith, NIH

text: lung

text: Mus musculus

text: SalI

text: NotI

text: CZECH II

text: spontaneous tumor, metastatic to mammary. Stem cell origin.

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: transgenic model WNT-1, expression driven by MMTV-LTR enhancer; Cloned unidirectionally. Primer: Oligo dT. Library constructed by Life Technologies. Investigator providing samples: Gilbert Smith, NIH

characteristic: lab-host

text: lung

text: Mus musculus

text: NotI

text: SalI

text: CZECH II

text: tumor, metastatic to mammary

characteristic: tissue-type

text: phagemid (ampicillin resistant)


text: University of Liverpool

text: live

text: Generic

text: fetal

text: Homo sapiens

text: retinal pigment epithelium

text: Uni-ZAP XR retinal pigment epithelium


text: Clemson University

text: live

text: Generic

characteristic: cultivar

text: supplier: Tanksley; Flower buds and flowers were taken from greenhouse plants (4-8 wks old, TA496). They were immediately frozen in liquid nitrogen and then size-separated while remaining frozen.

text: 0-3mm buds

text: Solanum lycopersicum

text: flower

text: tomato flower buds 0-3 mm, Cornell University

text: phagemid

text: pBlueScript SK(-)


text: Clemson University

text: live

text: Generic

characteristic: description

text: 3-8mm buds

text: Solanum lycopersicum

text: flower

text: tomato flower buds 3-8 mm, Cornell University

text: phagemid

text: pBlueScript SK(-)


text: Clemson University

text: live

text: Generic

text: buds 8mm-to-preanthesis

text: Solanum lycopersicum

text: flower

text: tomato flower buds 8 mm to pre-anthesis, Cornell University

text: phagemid

text: pBlueScript SK(-)

characteristic: vector


characteristic: External Id

text: Clemson University

text: live

text: Generic

text: anthesis

text: Solanum lycopersicum

text: flower

text: tomato flower buds, anthesis, Cornell University

text: phagemid

text: pBlueScript SK(-)


text: Clemson University

text: live

text: Generic

text: supplier: Giovannoni; Fruit were tagged at the breaker stage (first sign of lycopene accumulation on the blossom end of the fruit) and harvested 7 days post-breaker (fully red-ripe), 10 days post breaker, and 20 days post-breaker (over-ripe). 20 day fruit which showed external or internal signs of pathogenesis were discarded. Fruit were cut in half and the seeds and locules were discarded prior to freezing the pericarp.

text: red ripe (7-20 days post-breaker)

text: Solanum lycopersicum

characteristic: organism

text: pericarp

text: tomato fruit red ripe, TAMU

text: phagemid

text: pBlueScript SK(-)


text: Clemson University

characteristic: INSDC center name

text: live

text: Generic

text: supplier: Tanksley; Tissue supplied by Dave Garvin (USDA-ARS, Ithaca, NY 14850).

text: seedlings 5 days post-imbibition

text: Solanum lycopersicum

text: radicle

text: tomato radicle, 5 d post-imbibition, Cornell University

text: phagemid

text: pBlueScript SK(-)


text: Clemson University

text: live

text: Generic

text: plants in pre-anthesis stage

text: Solanum lycopersicum

text: root

text: tomato root, plants pre-anthesis, Cornell University

characteristic: title

text: phagemid

text: pBlueScript SK(-)


text: Clemson University

characteristic: INSDC first public

text: live

text: Generic

text: plants during and after fruit-set

text: Solanum lycopersicum

text: root

text: tomato root during/after fruit set, Cornell University

text: phagemid

text: pBlueScript SK(-)


text: Beijing Medical University

characteristic: INSDC last update

text: live

text: Generic

text: Electroacupuncture induces differential analgesia from individual to another one. This kind difference of electroacupuncture-analgesia is divided into two groups: non-responder and responder. Genes were identified by mRNA differential display. L3 and L4 are from non-responders; H1 and H4 are isolated from responder rats.

text: Rattus norvegicus

text: Wistar

text: Rattus norvegicus Wist


text: Universidad Nacional de Tucuman, INSIBIO, CONICET-UNT

text: live

text: Generic

text: Rattus norvegicus

text: Wistar

text: Rattus norvegicus Wistar


text: Institute of microbiology and epidemiology

text: live

text: Generic

text: Plasmodium berghei

text: Plasmodium berghei cDNA library


text: Graduate School of Science, Hokkaido University

text: live

text: Generic

text: Arabidopsis thaliana

text: Columbia

text: Arabidopsis thaliana Columbia


text: Kazusa DNA Research Institute

text: live

text: Generic

text: photoautotrophic growth

text: Chlamydomonas reinhardtii

text: EcoRI

text: XhoI

text: Chlamydomonas reinhardtii C9

text: pBluescriptII SK-


text: University of Toronto

text: live

text: Generic

text: Homo sapiens

text: Breast Cancer Associated Library


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Library constructed by Life Technologies. Investigator providing samples: Gilbert Smith, NIH

text: 5 months

text: mammary

characteristic: organ

text: Mus musculus

text: SalI

text: NotI

text: tumor, biopsy sample

text: phagemid (ampicillin resistant)


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

characteristic: INSDC status

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Library constructed by Life Technologies. Investigators providing samples: Lothar Hennighausen/Priscilla Furth, NIH Reference for transgenic model: Li et al., Cell Growth and Differentiation 7, 3-11 (1996).

text: 5 months

text: mammary

text: Mus musculus

text: SalI

text: NotI

text: NMRI

text: tumor, gross tissue

text: phagemid (ampicillin resistant)


text: University of Minnesota, College of Veterinary Medicine

text: live

text: Generic

text: PRRSV-infected macrophage

characteristic: cell type

text: From differential display analysis

text: Sus scrofa

text: Sus scrofa alveoli macrophage cDNA

text: plasmid

text: pBlueScript II SKplus


text: Jikei University School of Medicine

text: live

text: Generic

text: neuroblastoma

text: SK-N-SH

characteristic: cell-line

text: T/A cloning of cDNA from differentially displayed mRNA

text: Homo sapiens

text: female

characteristic: sex

text: cDNA Library from Human Neuroblastoma SK-N-SH

text: Plasmid


text: Jikei University School of Medicine

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: neuroepithelioma

text: SK-N-MC

text: Homo sapiens

text: female

text: cDNA Library from Human Neuroepithelioma SK-N-MC

text: Plasmid


text: live

text: Generic

text: cDNA made by oligo-dT priming. Non-directionally cloned into the UDG sites of pAMP10. Size-selected on agarose gel, average insert size 500 bp. Primary library; non-amplified. cDNA Library Preparation: David B. Krizman, Ph.D (NCI). Reference: Krizman et al. (1996) Cancer Research 56:5380-5383.

characteristic: lab-host

text: Homo sapiens

text: well-differentiated invasive carcinoma, floor of mouth

characteristic: tissue-type

text: plasmid (ampicillin resistant)


text: live

text: Generic

characteristic: description

text: Homo sapiens

text: normal squamous epithelium from retromolar trigone

text: plasmid (ampicillin resistant)


text: live

text: Generic

text: Homo sapiens

text: carcinoma in situ from retromolar trigone

text: plasmid (ampicillin resistant)

characteristic: vector


characteristic: External Id

text: University of Delaware

text: live

text: Generic

text: MDV infected splenic T cell

text: E.coli DH5 alpha

text: Gallus gallus

text: Male and Female

text: chicken MDV infected T cell cDNA library


text: University of Delaware

text: live

text: Generic

text: splenic T cell

text: E.coli DH10B

text: Gallus gallus

characteristic: organism

text: Male and Female

text: chicken T cell cDNA library


characteristic: INSDC center name

text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Library constructed by Life Technologies. Investigators providing samples: Lothar Hennighausen/Robin Humphreys, NIH

text: 7 months

characteristic: dev-stage

text: mammary

text: Mus musculus

text: SalI

text: NotI

text: mix FVB/N, C57BL/6J

characteristic: strain

text: tumor, gross tissue

text: phagemid (ampicillin resistant)


text: Bio College Kyoto

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: seedling

text: Vigna angularis

text: epicotyl

text: Vigna angularis seedling epicotyl

characteristic: title


text: University of Glasgow

text: live

characteristic: INSDC status

text: Generic

text: Brugia pahangi

text: L3 cDNA