text: live

text: Generic

text: sequence obtained by DDRT-T-PCR

text: Homo sapiens

text: vulva squamous carcinoma

text: Human vulva squamous carcinoma A431


status: PUBLIC

characteristic: External Id

text: Univ. of California Irvine

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: From exon amplification of cosmid contig DNA from between loci RPS14 and ANX6

characteristic: description

text: Homo sapiens

characteristic: organism

text: Human exon amplification Treacher Collins gene region

characteristic: title


text: VA Medical Center

text: live

text: Generic

text: multi-tissue normalized short-fragment cDNA library used in cDNA selection experiments per Parimoo et al., 1991, Proc. Natl. Acad. Sci. USA, 88: 9623-9627.

text: pooled

tag: attribute

characteristic: organ

text: Homo sapiens

text: multi-tissue

characteristic: tissue-type

text: multi-tissue normalized short-fragment

text: phage

text: lambda gt10

characteristic: vector


text: University of Maryland Baltimore County

text: live

text: Generic

text: somatic embryo

characteristic: cell type

text: Carrot somatic embryos at the globular stage

text: globular stage

characteristic: dev-stage

text: Daucus carota

text: Danver Half-long

characteristic: strain

text: Carrot somatic embryos


text: University of Wisconsin-Madison

text: live

text: Generic

text: insulinoma

characteristic: cell-line

characteristic: lab-host

text: Rattus norvegicus

text: Spraque-Dawley

text: Rat RINm5F insulinoma cell

text: Phage

text: Lambda gt11


characteristic: External Id

text: IMBB, Foundation for Reseach and Technology

characteristic: INSDC center name

text: live

text: Generic

text: Female adult. isolated by differential display of cDNA from midgut mRNA; specifically expressed in midgut as shown by Northern analysis; amplified by random 10-mer primer and oligo-T primer

text: adult

text: midgut

text: Anopheles gambiae

text: female

characteristic: sex

text: African malaria mosquito GDimopoulos


text: Myriad Genetics, Inc.

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Homo sapiens

text: female

text: normal breast

text: normal female breast tissue


text: VA Medical Center

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Fetal brain cDNA library from Clontech (cat# HL30036).

characteristic: description

text: fetal

text: brain

text: Homo sapiens

text: brain

text: fetal brain cDNA

text: phage

text: lambda gt11


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5'-TGTTACCAATCTGAAGTGGGAGCGGCCGCAATTTTTTTTTTTTTTTTTT-3'], double-stranded cDNA was size selected, ligated to Eco RI adapters (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of a modified pT7T3 vector (Pharmacia). Library went through one round of normalization to a Cot = 5. Library constructed by Bento Soares and M.Fatima Bonaldo. This library was constructed from the same fetus as the fetal heart library, Soares fetal heart NbHH19W.

text: 19 weeks

text: DH10B (ampicillin resistant)

text: lung

characteristic: organ

text: Homo sapiens

characteristic: organism

text: Not I

text: Eco RI

characteristic: title

text: Phagemid

text: pT7T3D (Pharmacia) with a modified polylinker


text: live

text: Generic

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5'-TGTTACCAATCTGAAGTGGGAGCGGCCGCACCAATTTTTTTTTTTTTTTTTTTTTTTTT-3'], double-stranded cDNA was size selected, ligated to Eco RI adapters (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of a modified pT7T3 vector (Pharmacia). Library went through one round of normalization to a Cot = 5. Library constructed by Bento Soares and M.Fatima Bonaldo. RNA from sporadic parathyroid adenomas was kindly provided by Dr. Stephen Marx, National Institute of Diabetes and Digestive and Kidney Diseases, NIH.

text: adult

text: DH10B (ampicillin resistant)

text: parathyroid gland

text: Homo sapiens

text: Not I

text: Eco RI

text: parathyroid tumor

characteristic: tissue-type

text: phagemid

characteristic: vector


text: Institute of Basic Medical sciences, Peking Union Medical College & Chinese Academy of Medical Sciences

text: live

text: Generic

text: Total RNA was isolated from 8-month and 3-month human fetal cerebrum and cerebellum. Then Differential Display RT-PCR was conducted. The cDNA PCR product of differentially expressional signifance was cloned and sequenced by employing pBluescript SK as vector.

text: fetal, 8-month and 3-month

characteristic: dev-stage

text: E.coli DH5a

text: Homo sapiens

text: cerebrum and cerebellum

text: Human fetal brain QBoqin2

text: Phagemid

text: pBluescript SK


text: Chonbuk National University

text: live

text: Generic

text: Hull cell

characteristic: cell type

text: 3'-directed cDNA clones; single-pass sequencing

text: sexual

text: Aspergillus nidulans

characteristic: strain

text: cleistothecium

text: Aspergillus nidulans cleistothecium


characteristic: External Id

text: National Eye Institute, NIH

text: live

text: Generic

text: Expressed sequence tags (first pass sequencing) from randomly selected bacteriophage clones (mRNA-cDNA) from human retinal fovea. The library is age normalized from ten sets of donor foveae 2-79 years old.

text: Homo sapiens

text: retinal fovea

text: Human fovea cDNA


text: Integrated Genetics

characteristic: INSDC center name

text: live

text: Generic

text: Exons were isolated from human chromosome 16p13.3 specific P1 clones using exon amplification (Nat. Genet. 6: 98-105, 1994). Amplified exons were cloned into pAMP10 using UDG and CUA-tailed secondary PCR primers (Gibco/BRL).

text: E. coli DH5a

characteristic: lab-host

text: Homo sapiens

text: Chromosome 16p13.3 Exon


text: Washington University School of Medicine

characteristic: INSDC first public

text: live

text: Generic

text: Reference: Hum Mol Gen 2, 1795 (1993) Takeda et al. Cloned unidirectionally. Primer: Oligo dT. ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'

text: SOLR cells (kanamycin resistant)

text: pancreas

text: Homo sapiens

text: EcoRI

text: XhoI

text: pancreatic islet

text: Pancreatic Islet

text: phagemid (ampicillin resistant)

text: pBluescript SK-


characteristic: INSDC last update

text: live

text: Generic

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCATTTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was size selected, ligated to Eco RI adapters (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of a modified pT7T3 vector (Pharmacia). Library went through one round of normalization to a Cot = 5. Library constructed by Bento Soares and M.Fatima Bonaldo.

text: DH10B (ampicillin resistant)

text: Homo sapiens

text: Not I

text: Eco RI

text: senescent fibroblast

text: pT7T3D (Pharmacia) with a modified polylinker V_TYPE: phagemid


text: live

characteristic: INSDC status

text: Generic

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCATCTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was size selected, ligated to Eco RI adapters (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of a modified pT7T3 vector (Pharmacia). Library went through one round of normalization to a Cot = 5. Library constructed by M.Fatima Bonaldo. This library was constructed from the same fetus as the fetal lung library, Soares fetal lung NbHL19W.

text: 19 weeks

text: DH10B (ampicillin resistant)

text: heart

text: Homo sapiens

text: Not I

text: Eco RI

text: phagemid


text: Michigan State University

text: live

text: Generic

characteristic: NCBI submission model

text: Using 5 ug of polyadenylated mRNA from 3 day-old Arabidopsis thaliana (Columbia) seedling hypocotyls as template and oligo d(t) as primer, first strand synthesis was catalyzed by Moloney murine leukemia virus reverse transcriptase (Pharmacia). Second-strand cDNA was made using the procedure of Gubler and Hoffman (1983) except that DNA ligase was omitted. After the second strand reaction, the ends of the cDNA were made blunt with Klenow fragment and EcoRI/NotI adapters (Pharmacia) were ligated to each end. The cDNA was purified from unligated adapters by spun-column chromatography using sephacryl s-300 and size-fractionated on a 1% low melting point mini-gel. Size selected cDNAs (0.5 - 1 kb) were removed from the gel using agarase (New England Biolabs), phenol:choloroform extracted and precipitated using 0.3 M NaOAc (pH 7)/ethanol. A portion of each cDNA size-fraction (0.1 ug) was co-precipitated with 1 ug of lambdaZapII (StratageneEcoRI digested, dephosphorylated arms and then ligated in a volume of 4 ul overnight. Each ligation mix was packaged in vitro using Gigapack II gold packaging extract (Stratagene). We have determined that although first strand cDNA synthesis was initiated using dT, almost all of the cDNAs begin 8-10 bp from the poly-A tail. The reason for the loss of the poly-A tail is most likely due to lower than anticipated nucleotide levels during the Klenow repair of ragged ends before the addition of linkers (3'-5' exo instead of 5'-3' pol). When this library is used please reference the ABRC and: Kieber, J. et al. (1993) Cell 72:427-441.

text: 3 day-old

text: Arabidopsis thaliana

text: EcoRI

text: EcoRI

text: Columbia

text: seedling hypocotyl

text: pBluescript SK-


text: Michigan State University

text: live

text: Generic

characteristic: NCBI submission package

text: Using 5 ug of polyadenylated mRNA from 3 day-old Arabidopsis thaliana (Columbia) seedling hypocotyls as template and oligo d(t) as primer, first strand synthesis was catalyzed by Moloney murine leukemia virus reverse transcriptase (Pharmacia). Second-strand cDNA was made using the procedure of Gubler and Hoffman (1983) except that DNA ligase was omitted. After the second strand reaction, the ends of the cDNA were made blunt with Klenow fragment and EcoRI/NotI adapters (Pharmacia) were ligated to each end. The cDNA was purified from unligated adapters by spun-column chromatography using sephacryl s-300 and size-fractionated on a 1% low melting point mini-gel. Size selected cDNAs (1 - 2 kb) were removed from the gel using agarase (New England Biolabs), phenol:choloroform extracted and precipitated using 0.3 M NaOAc (pH 7)/ethanol. A portion of each cDNA size-fraction (0.1 ug) was co-precipitated with 1 ug of lambdaZapII (StratageneEcoRI digested, dephosphorylated arms and then ligated in a volume of 4 ul overnight. Each ligation mix was packaged in vitro using Gigapack II gold packaging extract (Stratagene). We have determined that although first strand cDNA synthesis was initiated using dT, almost all of the cDNAs begin 8-10 bp from the poly-A tail. The reason for the loss of the poly-A tail is most likely due to lower than anticipated nucleotide levels during the Klenow repair of ragged ends before the addition of linkers (3'-5' exo instead of 5'-3' pol). When this library is used please reference the ABRC and: Kieber, J. et al. (1993) Cell 72:427-441.

text: 3 day-old

text: Arabidopsis thaliana

text: EcoRI

text: EcoRI

text: Columbia

text: seedling hypocotyl

text: pBluescript SK-


text: Michigan State University

text: live

text: Generic

text: Using 5 ug of polyadenylated mRNA from 3 day-old Arabidopsis thaliana (Columbia) seedling hypocotyls as template and oligo d(t) as primer, first strand synthesis was catalyzed by Moloney murine leukemia virus reverse transcriptase (Pharmacia). Second-strand cDNA was made using the procedure of Gubler and Hoffman (1983) except that DNA ligase was omitted. After the second strand reaction, the ends of the cDNA were made blunt with Klenow fragment and EcoRI/NotI adapters (Pharmacia) were ligated to each end. The cDNA was purified from unligated adapters by spun-column chromatography using sephacryl s-300 and size-fractionated on a 1% low melting point mini-gel. Size selected cDNAs (2 - 3 kb) were removed from the gel using agarase (New England Biolabs), phenol:choloroform extracted and precipitated using 0.3 M NaOAc (pH 7)/ethanol. A portion of each cDNA size-fraction (0.1 ug) was co-precipitated with 1 ug of lambdaZapII (StratageneEcoRI digested, dephosphorylated arms and then ligated in a volume of 4 ul overnight. Each ligation mix was packaged in vitro using Gigapack II gold packaging extract (Stratagene). We have determined that although first strand cDNA synthesis was initiated using dT, almost all of the cDNAs begin 8-10 bp from the poly-A tail. The reason for the loss of the poly-A tail is most likely due to lower than anticipated nucleotide levels during the Klenow repair of ragged ends before the addition of linkers (3'-5' exo instead of 5'-3' pol). When this library is used please reference the ABRC and: Kieber, J. et al. (1993) Cell 72:427-441.

characteristic: description

text: 3 day-old

text: Arabidopsis thaliana

text: EcoRI

text: EcoRI

text: Columbia

text: seedling hypocotyl

text: pBluescript SK-


text: Michigan State University

text: live

text: Generic

text: Using 5 ug of polyadenylated mRNA from 3 day-old Arabidopsis thaliana (Columbia) seedling hypocotyls as template and oligo d(t) as primer, first strand synthesis was catalyzed by Moloney murine leukemia virus reverse transcriptase (Pharmacia). Second-strand cDNA was made using the procedure of Gubler and Hoffman (1983) except that DNA ligase was omitted. After the second strand reaction, the ends of the cDNA were made blunt with Klenow fragment and EcoRI/NotI adapters (Pharmacia) were ligated to each end. The cDNA was purified from unligated adapters by spun-column chromatography using sephacryl s-300 and size-fractionated on a 1% low melting point mini-gel. Size selected cDNAs (3 - 6 kb) were removed from the gel using agarase (New England Biolabs), phenol:choloroform extracted and precipitated using 0.3 M NaOAc (pH 7)/ethanol. A portion of each cDNA size-fraction (0.1 ug) was co-precipitated with 1 ug of lambdaZapII (StratageneEcoRI digested, dephosphorylated arms and then ligated in a volume of 4 ul overnight. Each ligation mix was packaged in vitro using Gigapack II gold packaging extract (Stratagene). We have determined that although first strand cDNA synthesis was initiated using dT, almost all of the cDNAs begin 8-10 bp from the poly-A tail. The reason for the loss of the poly-A tail is most likely due to lower than anticipated nucleotide levels during the Klenow repair of ragged ends before the addition of linkers (3'-5' exo instead of 5'-3' pol). When this library is used please reference the ABRC and: Kieber, J. et al. (1993) Cell 72:427-441.

text: 3 day-old

text: Arabidopsis thaliana

text: EcoRI

text: EcoRI

text: Columbia

text: seedling hypocotyl

text: pBluescript SK-


text: Leeds University

text: live

text: Generic

text: adult

text: Homo sapiens

text: normal gingiva

text: Human normal gingiva


text: German Cancer Research Center

text: live

text: Generic

text: identified by cDNA-RDA

text: Homo sapiens

text: Human pancreatic tumor


text: Washington University School of MedicineP

text: live

text: Generic

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCATTTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was size selected, ligated to Eco RI adapters (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of a modified pT7T3 vector (Pharmacia). Library went through one round of normalization to a Cot = 5. Library constructed by Bento Soares and M.Fatima Bonaldo. RNA was kindly provided by Dr. Minoru Ko (Wayne State University).

text: 19.5 dpc total fetus

text: DH10B (ampicillin resistant)

text: Mus musculus

text: Not I

text: Eco RI

text: Soares mouse p3NMF19.5

text: phagemid


text: Johns Hopkins School of Medicine

text: live

text: Generic

text: The library used for sequencing was a sublibrary derived from a human retina cDNA library. Inserts from retina cDNA library DNA were isolated, cleaved with Tsp509I, size selected, and cloned into lamda gt10. Individual plaques were arrayed and used as templates for PCR amplification and these PCR products were used for sequencing.

text: adult

text: E. coli strain K802

text: eye

characteristic: organ

text: Homo sapiens

text: EcoRI

text: EcoRI

text: mixed (males and females)

characteristic: sex

text: retina

text: Human retina cDNA Tsp509I-cleaved sublibrary

text: lambda gt10


text: Johns Hopkins School of Medicine

text: live

text: Generic

text: The library used for sequencing was a sublibrary derived from a human retina cDNA library. Inserts from retina cDNA library DNA were isolated, randomly primed, PCR amplified, size-selected, and cloned into lambda gt10. Individual plaques were arrayed and used as templates for PCR amplification, and these PCR products were used for sequencing.

text: adult

text: E. coli strain K802

text: eye

text: Homo sapiens

characteristic: organism

text: EcoRI

text: EcoRI

text: mixed (males and females)

text: retina

text: Human retina cDNA randomly primed sublibrary

characteristic: title

text: lambda gt10


text: Universitatsklinikum Essen GSH-Essen

text: live

text: Generic

text: of vectorLambda ZapII Express Library

text: embryonic

text: Homo sapiens

text: EcoR1 Restriction enzyme at site

text: EcoR1 Restriction enzyme at site

text: male and female

text: Caucasian

text: fetal brain

characteristic: tissue-type

text: Human fetal brain, Stratagene

text: Phage

text: Lambda ZapII

characteristic: vector


text: Duke University

text: live

text: Generic

text: thymus

text: Mus musculus

text: thymus

text: Mouse thymus expressed sequences


text: Institute of Biomedical and Life Sciences, Division of Molecular Genetics

text: live

text: Generic

text: Mouse Ventral Midbrain directional cDNA library in pSPORT1. The library was created by subtractive hybridisation using VM mRNA as the target nucleic acid population and total cerebellar ss cDNA as the driver nucleic acid population. The clones are 3' directed.

text: brain

text: Mus musculus

text: ventral midbrain

text: Mouse VM cDNA library

text: Plasmid


text: live

text: Generic

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCGGAAATTTTTTTTTTTTTTTTTTTTTTTTT 3'], on equal amounts of mRNA from 2 13.5dpc and 2 14.5dpc embryos [total RNA provided by Minoru Ko, Wayne State Univ., from 2 ]; double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library went through one round of normalization, and was constructed by Bento Soares and M.Fatima Bonaldo.

text: 13.5-14.5dpc total fetus

characteristic: dev-stage

text: Mus musculus

text: Not I

text: Eco RI

text: embryo

text: Soares mouse embryo NbME13.5 14.5

text: phagemid (ampicillin resistant)


text: Medical Genetics Service Hospital, I.R.C.C.S. 'CASA Soll. Sofferenza'

text: live

text: Generic

text: Homo sapiens

text: Human PGasparini


text: Washington University School of Medicine

text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. pCMV-SPORT2 vector.

text: adult

text: brain

text: Mus musculus

text: SalI

text: NotI

characteristic: strain

text: brain

text: Life Tech mouse brain

text: phagemid (ampicillin resistant)


characteristic: External Id

text: Cornell University

text: live

text: Generic

text: ES cells

characteristic: cell type

text: Bruce 4

characteristic: cell-line

text: embryonic

text: cell line

text: Mus musculus

text: Mouse Bruce 4 cell line


text: NIH, NIMH, DIRP, CNG

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: Homo sapiens

text: Human brain ARSanders


text: University of Bielefeld

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: adult

text: testis

text: Mus musculus

text: male

text: Mouse testis cDNA (Stratagene)


text: NIH

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: OsA-CL

text: Homo sapiens

text: osteosarcoma

text: Human osteosarcoma EGracia


text: Universitaet des Saarlandes

text: live

text: Generic

text: Homo sapiens

text: glioblastoma

text: Human glioblastoma UFischer


text: live

text: Generic

text: Toxoplasma parasites were grown in human foreskin fibroblasts cultures in vitro. The library was constructed by A. Hehl and I. Manger, Stanford University. cDNAs were synthesized from polyA mRNAs by oligo d(T) priming and directionally cloned into the EcoRI and XhoI sites of the Lambda Zap vector using the ZAP-cDNA synthesis Kit (Statagene). Warning: the library contains a small percentage of human cDNAs derived from the human host cells.

characteristic: description

text: XL1-Blue MRF'

characteristic: lab-host

text: Toxoplasma gondii

text: EcoRI

text: XhoI

text: ME49, clone PDS

text: TgME49 Tachyzoite cDNA

text: phagemid

text: Lambda ZAP


text: University of British Columbia

text: live

text: Generic

text: Homo sapiens

characteristic: organism

text: lung adenocarcinoma

text: Human lung adenocarcinoma A549


text: McGill University

text: live

text: Generic

text: The library is commercially available from Stratagene Ltd. The liver tissue sample was collected from broiler-breeders of 7 weeks. The messenger RNA was purified with oligo(dt). The average size of inserts was 1.0kb.

text: liver

characteristic: organ

text: Gallus gallus

text: EcoRI

text: XhoI

text: male

characteristic: sex

text: broiler-breeder

text: chicken liver cDNA library, Stratagene (cat.#935402)

characteristic: title

text: phage

text: Lambda ZAP


text: Institut Pasteur de Lille

text: live

text: Generic

text: adult worm

characteristic: dev-stage

text: Schistosoma mansoni

text: male, female

text: Puerto-Rican

text: Schistosoma mansoni adult worm Lambda ZAP II library

text: Lambda ZAP II

characteristic: vector


characteristic: External Id

text: Chinese National Human Genome Center at Shanghai

text: live

text: Generic

text: Several hundred adult Schistosoma japonicum (Anhui, P.R. China, strain), of mixed sex, were perfused from the mesenteries of experimentally infected mice and rabbits at the Queensland Institute of Medical Research, Brisbane, Australia (QIMR), and stored for several months in liquid nitrogen. Subsequently, mRNA was isolated at the QIMR from lysates of these worms by oligo dT chromatography, using a kit from Pharmacia. The mRNA was then shipped to Clontech, Palo Alto, CA, USA, who constructed a cDNA library. First strand synthesis was primed with an oligo-dT-XhoI-primer and synthesized using M-MLV reverse transcriptase. Second strand synthesis was accomplished with RNAse H and T4 DNA polymerase. The double stranded cDNA was ligated to EcoRI linkers, digested with EcoRI and XhoI, and ligated into the phagemid vector lambda ZAP II XR. After construction of this directional library by Clontech, it was returned to the QIMR. During analysis of the library at the QIMR, we have found that a small percentage, 2% to 3%, of the clones contain inserts that appear to be highly homologous to sequences from salmonoid fishes, as determined by homology comparisons using BLAST and by Southern hybridization analysis to genomic DNA from salmon (Sigma Chemical Co., St. Louis, MO) under stringent washing conditions. The remainder of the clones appear to contain S. japonicum sequences.

text: Adult worms

text: Mouse and rabbit

text: Schistosoma japonicum

text: EcoR I

text: Xhol I

text: Male and female

text: Chinese (Anhui) strain

characteristic: strain

text: Whole body

characteristic: tissue-type

text: Adult SjC 7/94

text: Phagemid

text: Lambda ZAP-II XR.


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

characteristic: INSDC center name

text: live

text: Generic

text: This is a subtracted version of the original Soares fetal liver spleen 1NFLS library. 1st strand cDNA was primed with a Pac I - oligo(dT) primer [5' AACTGGAAGAATTAATTAAAGATCTTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Pac I and cloned into the Pac I and Eco RI sites of the modified pT7T3 vector. Library went through one round of normalization. Library constructed by Bento Soares and M.Fatima Bonaldo.

text: 20 week-post conception fetus

text: DH10B (ampicillin resistant)

text: Liver and Spleen

text: Homo sapiens

text: Pac I

text: Eco RI

text: male

text: phagemid

text: pT7T3D (Pharmacia) with a modified polylinker


text: BIOS Laboratories

characteristic: INSDC first public

text: live

text: Generic

text: mononuclear cells

characteristic: cell type

text: Homo sapiens

text: umbilical cord blood

text: Human cord blood mononuclear cell mRNA


text: University of Miami School of Medicine

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Mus musculus

text: BALB/c

text: testes

text: Mouse testes (ZTrachtulec)


text: Baylor College of Medicine

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Mus musculus

text: Mouse chromosome X (MLLevin)


text: Rigshospitalet, Institute for Inflammation Research

text: live

text: Generic

text: mononuclear cells

text: Homo sapiens

text: Human mononuclear cells (KRieneck)


text: Deutsches Krebsforschungszentrum (DKFZ)

text: live

text: Generic

text: Homo sapiens

text: Human (NSHeiss)


text: University of Edinburgh

text: live

text: Generic

text: Mus musculus

text: NIH Swiss

text: brain

text: Mouse brain ATCC 37431


text: Medical Research Council (UK) or Universitaet zu Koeln (D)

text: live

text: Generic

text: cDNA library from Tl[10b] embryos minus cDNAs from Tl[r444]/Tl[9QRX] embryos

characteristic: description

text: Drosophila melanogaster

text: V cDNA subtractive library

text: pBluescript II


text: MRC Human Biochemical Genetics Unit

text: live

text: Generic

text: 9.5 d.p.c.

text: notochord

text: Mus musculus

characteristic: organism

text: C57BL/6 x DBA

text: notochord

text: Mouse notochord 9.5 d.p.c.

text: lambda pExcell


text: Charite University Hospital, Humboldt University of Berlin

text: live

text: Generic

text: monocytes

text: Monocytes were prepared from blood by ficoll-hypaque, percoll and T cell rosetting purification steps (purity: 96 %). mRNA was prepared from activated monocytes from a patient with rheumatoid arthritis. mRNA was reverse transcribed with MuLV. Using Eco-RI linkers cDNA was cloned into l-gt-11 vector arms. The cDNA library was screened by differential hybidization using radioactively marked ss-cDNA from activated and non-activated monocytes.

text: blood

characteristic: organ

text: Homo sapiens

text: Eco-RI

text: male

text: RA-MO-III

text: Phage


text: Institut National de la Recherche Agronomique

text: live

text: Generic

text: DEA

characteristic: cultivar

text: cDNA library from poly(A)+ mRNA of 24-h glucose-starved maize root tips, as described in Chevalier, C., Bourgeois, E., Pradet, A., Raymond, P. (1995) Plant Molecular Biology Volume 28 Pages 473-485

text: Primary root

text: Zea mays

text: 3-mm-long tip

text: Glucose-starved maize root tip cDNA library

text: Lambda ZAPII


text: LLNL

text: live

text: Generic

text: Homo sapiens

text: Human (Fan,Wufang)

characteristic: title


text: National Institute of Genetics

text: live

text: Generic

text: One or more human adult tissue

text: adult

characteristic: dev-stage

text: Homo sapiens

text: Human adult (K.Okubo)


text: University of Tennessee, Knoxville, TN

text: live

text: Generic

text: Bragg

text: Double stranded cDNAs were synthesized by the method of Gubler and Hoffman (1983), using a cDNA synthesis kit (Amersham Life Science Inc.) and then ligated to an EcoRI adaptor (Amersham). cDNA library was constructed in lambda ZAPII vector using lambda ZAPII/EcoRI/Gigapack II cloning kit (Stratagene)

text: 22 day-old seedlings (14 days after inoculation with Bradyrhizobium japonicum strain USDA110)

text: Autoregulated shoots

text: Glycine max

text: Soybean Shoot cDNA Library

text: phage

text: lambda ZAPII

characteristic: vector


characteristic: External Id

text: Washington University School of Medicine

text: live

text: Generic

text: Toxoplasma parasites were grown in human foreskin fibroblast cultures in vitro. The library was constructed by A. Hehl and I. Manger, Stanford University. cDNAs were synthesized from polyA mRNAs by oligo d(T) priming and directionally cloned into the EcoRI and XhoI sites of the Lambda Zap vector using the ZAP-cDNA synthesis Kit (Statagene). Warning: the library contains a small percentage of human cDNAs derived from the human host cells

text: in vitro Bradyzoite, 6day

text: XL1-Blue MRF'

characteristic: lab-host

text: Toxoplasma gondii

text: EcoRI

text: XhoI

text: ME49, clone PDS

text: phagemid

text: Lambda ZAP


text: University of Chicago

text: live

text: Generic

text: cDNA obtained from pregnant rat myometrium on days 12, 16, 20, 21, 22, and postpartum day 1, using differential display

text: Rattus norvegicus

text: female

characteristic: sex

text: myometrium

characteristic: tissue-type

text: Rat myometrium, differential display


text: University of Tokyo

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: normalized directionally cloned cDNA from adult heart

text: adult

text: heart

text: Homo sapiens

text: Human heart cDNA (YNakamura)


text: Washington University School of MedicineP

characteristic: INSDC last update

text: live

text: Generic

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCGGAAATTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library went through one round of normalization, and was constructed by Bento Soares and M.Fatima Bonaldo.

text: adult

text: placenta

text: Mus musculus

text: Not I

text: Eco RI

characteristic: strain

text: placenta

text: Soares mouse placenta 4NbMP13.5 14.5

text: phagemid (ampicillin resistant)


text: International Livestock Research Institute

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: cDNAs were generated from poly (A+) enriched mRNA prepared from different developmental stages of T.b.brucei by reverse transcription followed by PCR amplification using mini-exon and oligo(dT) primers. The cDNA generated were utilized in RADES-PCR coupled with differential hybridisations to identify differentially expressed mRNA transcripts. The products which showed to be differentially expressed were cloned pGEM -T vector. These differentially expressed mRNA transcripts were (are being) sequenced to generate differentially expressed sequence tags.

text: Trypanosoma brucei brucei

text: Clone A4

text: Bloodstream form of serodeme ILTat1.1


text: Stanford University

text: live

text: Generic

characteristic: NCBI submission package

text: Library made by Steve Elledge (Baylor)

characteristic: description

text: Homo sapiens

text: whole peripheral blood

text: Human peripheral blood (Steve Elledge)

text: Lambda-Yes


text: Gunma Univ., Biosignal Research Center

text: live

text: Generic

text: 21 day old

text: Rattus norvegicus

characteristic: organism

text: female

text: Wistar

text: ovary

text: Rat 21 day old female ovary mRNA PMSG 3h


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' AACTGGAAGAATTCGCGGCCGCCTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library went through one round of normalization. Library constructed by M. Fatima Bonaldo.

text: adult

text: uterus

characteristic: organ

text: Homo sapiens

text: Not I

text: Eco RI

text: female

text: phagemid


text: Intitute for Human Genetics

text: live

text: Generic

text: Arrayed human placenta cDNA library constructed by direct cloning and oligo(dT)-priming of mRNA in Lambda ZAP II

text: Homo sapiens

text: Not I

text: Not I

text: male

text: placenta

text: HPLA CCLee

characteristic: title

text: Phage

text: Lambda ZAP II


text: University Ulm

text: live

text: Generic

text: Homo sapiens

text: pancreatic cancer

text: Human pancreatic cancer (CWallrapp)


text: Fondation Jean Dausset - CEPH

text: live

text: Generic

text: cDNA library of S. Meier-Ewert, Max Planck Inst.f.Mol.Genetics, Berlin, FRG

text: fetus

characteristic: dev-stage

text: Homo sapiens

text: brain

text: Human fetal brain S. Meier-Ewert


text: Beth Israel Hospital and Harvard Medical School

text: live

text: Generic

text: adult

text: Toxocara canis

text: wild-type

text: anterior regions

text: Toxocara canis adult mRNA


text: live

text: Generic

text: infective larva (L3) stage

text: Toxocara canis

text: wild-type

text: Toxocara canis stage L3 mRNA


text: Institute for Molecular and Cellular Regulation, Gunma University

text: live

text: Generic

text: mRNA was prepared from normal rat islets. cDNA was directionally synthesized from the Xho I in the vector to the EcoRI site

text: Rattus norvegicus

text: EcoRI

text: XhoI

text: pancreatic islet

text: Rat pancreatic islet cDNA

text: Lambda ZAPII

characteristic: vector


characteristic: External Id

text: Hospital for Sick Children

text: live

text: Generic

text: Homo sapiens

text: alveolar rhabdomyosarcoma

text: Human alveolar rhabdomyosarcoma


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size: 1.0 kb; Uni-ZAP XR Vector; ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'

text: 4 weeks

text: SOLR (kanamycin resistant)

characteristic: lab-host

text: kidney

text: Mus musculus

text: EcoRI

text: XhoI

text: females

text: kidney

text: Stratagene mouse kidney (#937315)

text: phagemid (ampicillin resistant)

text: pBluescript SK-


text: live

text: Generic

text: 10-12 week old

text: SOLR (kanamycin resistant)

text: testis

text: Mus musculus

text: EcoRI

text: XhoI

text: males

characteristic: sex

text: testis

text: Stratagene mouse testis (#937308)

text: phagemid (ampicillin resistant)

text: pBluescript SK-


text: KIST

text: live

text: Generic

text: hepatocyte

characteristic: cell type

text: derived by differential display PCR

text: Mus musculus

text: liver

characteristic: tissue-type

text: Mouse hepatocyte differential display


characteristic: INSDC first public

text: live

text: Generic

text: T84 carcinoma cell line

characteristic: cell-line

text: Cloned unidirectionally. Primer: Oligo dT. T-84 colonic epithelial cell line. Average insert size: 1.0 kb; Uni-ZAP XR Vector; ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'

text: SOLR cells (kanamycin resistant)

text: colon

text: Homo sapiens

text: EcoRI

text: XhoI

text: tumor

text: Stratagene colon (#937204)

text: phagemid

text: pBluescript SK-


text: Tokai University School of Medicine

characteristic: INSDC center name

characteristic: INSDC last update

text: live

text: Generic

text: mRNA of Trypanosoma cruzi Y strain, epimastigote was prepared with QuickPrep Micro mRNA purification kit(Pharmacia). 1st strand cDNA was primed with a NotI-oligo(dt) primer, double-stranded cDNA was ligated to EcoRI adapters with TimeSaver cDNA Synthesis Kit and Directional Cloning Toolbox(Pharmacia), and digested with NotI and cloned into the NotI and EcoRI sites of lambdaExCell(Pharmacia). Library was constructed by Tomoo Tanaka.

text: Trypanosoma cruzi

text: Y

characteristic: strain

text: Trypanosoma cruzi Y (Tomoo Tanaka)


text: Iowa State University

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Oligo (dT) primed

text: adult

text: Sus scrofa

text: spleen

text: Pig Spleen lambda gt 11 Library (Clontech Cat # PL1006b)


text: Washington University School of Medicine

text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Corneal fibroblasts grown from explants, 76 years. Average insert size: 1.5 kb; Uni-ZAP XR Vector; ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'

characteristic: description

text: 76 years

text: SOLR cells (kanamycin resistant)

text: cornea

characteristic: organ

text: Homo sapiens

characteristic: organism

text: EcoRI

text: XhoI

text: Stratagene corneal stroma (#937222)

text: phagemid

text: pBluescript SK-


text: National Institute of Bioscience and Human-Technology

text: live

text: Generic

text: endothelial

text: neonatal

characteristic: dev-stage

text: Homo sapiens

text: male

text: umbilical cord vein

text: Human endothelial cell (Y.Mitsui)

characteristic: title

text: pCMV-SPORT

characteristic: vector


characteristic: External Id

text: Iowa State University

text: live

text: Generic

text: library kindly provided by C. Louis, Univ. Minnesota

text: Adult

text: Sus scrofa

text: Pietrain breed

text: muscle, skeletal, longissimus dorsi

text: Pig Muscle Lambda ZAPII Library


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Pancreatic adenocarcinoma cell line. Average insert size: 1.0 kb; Uni-ZAP XR Vector; ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'

text: SOLR cells (kanamycin resistant)

characteristic: lab-host

text: pancreas

text: Homo sapiens

text: EcoRI

text: XhoI

text: Stratagene pancreas (#937208)

text: phagemid

text: pBluescript SK-


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. W138 cell line. Average insert size: 0.8 kb; Uni-ZAP XR Vector; ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'

text: SOLR cells (kanamycin resistant)

text: Homo sapiens

text: EcoRI

text: XhoI

text: Stratagene fibroblast (#937212)

text: phagemid

text: pBluescript SK-


text: INSERM

text: live

text: Generic

text: mRNA was prepared from human testis of a 27 years old man. cDNA was prepared using a 15mer oligo dT anchored by two degenerated bases at its 3'end and containing a NotI site at its 5'end. The cDNA was cloned between Sal1 and NotI sites of pSPORT1. The Mlu1-Sal1 fragement come from the adaptator used for the cloning. The 3' end is at the NotI site. cDNA corresponding to abundant species were eliminated from this library.

text: Homo sapiens

text: Testis 5

text: Plasmid


text: Washington University School of MedicineP

text: live

text: Generic

text: Cloned unidirectionally from mRNA prepared from diaphragm muscle. Primer: Oligo dT. Average insert size: 1.5 kb. Uni-ZAP XR Vector; ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'

text: adult

text: SOLR (kanamycin resistant)

text: diaphragm

text: Mus musculus

text: EcoRI

text: XhoI

text: diaphragm

characteristic: tissue-type

text: Stratagene mouse diaphragm (#937303)

text: phagemid (ampicillin resistant)

text: pBluescript SK-


characteristic: INSDC first public

text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. From M2 cells, a highly metastatic derivative of the K-1735 (mouse) melanoma. Average insert size: 1.0 kb; Uni-ZAP XR Vector; ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'

text: M2 cells

text: SOLR (kanamycin resistant)

text: skin

text: Mus musculus

text: EcoRI

text: XhoI

text: melanoma

text: Stratagene mouse melanoma (#937312)

text: phagemid (ampicillin resistant)

text: pBluescript SK-


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Whole skin from 11 week old C57BL/6 female mice. Average insert size: 1.0 kb; Uni-ZAP XR Vector; ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'

text: 11 weeks old

text: SOLR (kanamycin resistant)

text: skin

text: Mus musculus

text: EcoRI

text: XhoI

text: females

characteristic: sex

text: whole skin

text: Stratagene mouse skin (#937313)

text: phagemid (ampicillin resistant)

text: pBluescript SK-


characteristic: INSDC center name

text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. P19 cell line. Average insert size: 1.0 kb; Uni-ZAP XR Vector; ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'

text: embryonic

text: SOLR (kanamycin resistant)

text: Mus musculus

text: EcoRI

text: XhoI

text: carcinoma

text: Stratagene mouse embryonic carcinoma (#937317)

text: phagemid (ampicillin resistant)

text: pBluescript SK-


text: Univ. of Tokai School of Med.

characteristic: INSDC last update

text: live

text: Generic

text: fetal

text: Homo sapiens

text: brain

text: Human brain cDNA

text: lambda zapII


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. NT2 cells (Ntera-2/c1.D1) induced with Retinoic Acid for 24 hours. Average insert size: 1.5 kb; Uni-ZAP XR Vector; ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'

text: Ntera-2/RA neuroepithelial cells

text: SOLR (kanamycin resistant)

text: Homo sapiens

text: EcoRI

text: XhoI

text: Stratagene neuroepithelium (#937231)

text: phagemid (ampicillin resistant)

text: pBluescript SK-


text: University of Colorado Health Sciences Center

text: live

text: Generic

text: adult

text: lung

text: Homo sapiens

text: Clontech adult lung cDNA library (HL1158a)


text: Yale University

text: live

text: Generic

text: Homo sapiens

text: giant cell carcinoma of lung

text: Human (P.Hui)


text: Hanyang University

text: live

text: Generic

text: exocervical cells

characteristic: cell-line

text: Homo sapiens

text: Human exocervical cells (CGLee)


text: live

characteristic: INSDC status

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. WEHI-3 cell line. Average insert size: 1.5 kb; Uni-ZAP XR Vector; ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'

text: WEHI-3 cell line

text: SOLR (kanamycin resistant)

text: blood

text: Mus musculus

text: EcoRI

text: XhoI

text: macrophage

text: Stratagene mouse macrophage (#937306)

text: phagemid (ampicillin resistant)

text: pBluescript SK-


text: Otsuka Pharmaceutical Co.,Ltd

text: live

text: Generic

characteristic: NCBI submission model

text: Homo sapiens

text: placenta

text: Human placenta cDNA (TFujiwara)


text: live

text: Generic

characteristic: NCBI submission package

text: Cloned unidirectionally. Primer: Oligo dT. P19 cell line treated with retinoic acid. Average insert size: 1.0 kb; Uni-ZAP XR Vector; ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'

text: embryonic

text: SOLR (kanamycin resistant)

text: Mus musculus

text: EcoRI

text: XhoI

text: carcinoma

text: Stratagene mouse embryonic carcinomaRA (#937318)

text: phagemid (ampicillin resistant)

text: pBluescript SK-


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. 93 pooled NIH/Swiss 13 day embryo hearts. Average insert size: 1.0 kb; Uni-ZAP XR Vector; ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'

characteristic: description

text: 13 day embryos

text: SOLR (kanamycin resistant)

text: heart

text: Mus musculus

text: EcoRI

text: XhoI

text: pooled

text: NIH Swiss

characteristic: strain

text: heart

text: Stratagene mouse heart (#937316)

text: phagemid (ampicillin resistant)

text: pBluescript SK-


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Papillary serous carcinoma, isolated from ascites, 64 year old caucasian. Average insert size: 0.8 kb; Uni-ZAP XR Vector; ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'

text: adult, 64 years

text: SOLR (kanamycin resistant)

text: Homo sapiens

characteristic: organism

text: EcoRI

text: XhoI

text: female

text: Stratagene ovarian cancer (#937219)

text: phagemid (ampicillin resistant)

text: Bluescript SK-


text: Sidney Kimmel Cancer Center

text: live

text: Generic

text: 23132 gastric carcinoma cell line

text: Homo sapiens

text: Human 23132 gastric carcinoma cell line


text: Institute of Plant Genetics, Polish Academy of Sciences

text: live

text: Generic

text: mRNA was isolated from cold hardened plantlets of Solanum sogarandinum. The cDNA library was constructed acc. to manufacturer's instructions (Stratagene, Heidelberg). The cDNA was directionally cloned with 5' end proximal to the EcoRI site end 3' end proximal to the XhoI site.

text: Two weeks old plantlets

characteristic: dev-stage

text: Solanum sogarandinum

text: EcoRI

text: XhoI

text: Potato Lambda Uni Zap cDNA Library

text: Lambda ZapII


text: National Human Genome Research Institute/NIH

text: live

text: Generic

text: cDNA was generated from cytoplasmic RNA using a mixture of random DNA hexamers and oligo(dT). From this pool of cDNA, human chromosome 7-enriched cDNA was isolated by direct cDNA selection using chromosome 7 genomic DNA (cosmids). The resulting direct-selected cDNA was cloned into a plasmid vector using a non-directional uracil DNA glycosylase (UDG)-mediated cloning strategy.

text: pool of 9 week and 12 week

text: E. coli strain DH5 alpha

text: brain

characteristic: organ

text: Homo sapiens

text: female and male mixture

text: brain

text: Chromosome 7 Fetal Brain cDNA Library

characteristic: title

text: plasmid


characteristic: External Id

text: live

text: Generic

text: placenta obtained at birth (full term)

text: E. coli strain DH5 alpha

text: placenta

text: Homo sapiens

text: female

text: Chromosome 7 Placental cDNA Library

text: plasmid

characteristic: vector


text: live

text: Generic

text: HeLa cell line; ATCC

text: E. coli strain DH5 alpha

characteristic: lab-host

text: Homo sapiens

text: female

text: Chromosome 7 HeLa cDNA Library

text: plasmid


characteristic: INSDC first public

text: live

text: Generic

text: 1 week old (male), 11 year old (female)

text: E. coli strain DH5 alpha

text: thymus

text: Homo sapiens

text: mixture of female and male

text: thymus

characteristic: tissue-type

text: Chromosome 7 Thymus cDNA Library

text: plasmid


text: Washington University School of Medicine

characteristic: INSDC center name

text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. From adenocarcinoma HT-29 (HTB 38), moderately differentiated grade II. Average insert size: 1.5 kb; Uni-ZAP XR Vector; ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'

text: HT-29 (HTB 38) adenocarcinoma

text: SOLR (kanamycin resistant)

text: Homo sapiens

text: EcoRI

text: XhoI

text: Stratagene colon HT29 (#937221)

text: phagemid (ampicillin resistant)

text: pBluescript SK-


text: NIH

characteristic: INSDC last update

text: live

text: Generic

text: Lymphatic filarial nematode parasite of humans. mRNA was prepared from approximately 85,000 microfilariae isolated from the blood of an infected individual from Guyana, South America and converted to double-stranded cDNA using reverse transcriptase and oligo(dT) followed by RNase H and DNA pol I. The library has 2.9 x 10E5 independent recombinants and the average insert size is ~1kb. The library was constructed by Sandra J. Laney. The library is available from Dr.S.A.Williams, email: genome@smith.edu.

text: microfilaria

text: XL1-Blue MRF'

text: Wuchereria bancrofti

text: Eco RI

text: Xho I

text: Wuchereria bancrofti microfilaria cDNA (SAW95SjL-WbMf)

text: Phage

text: Lambda Uni-ZAP XR


text: Washington University School of MedicineP

text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. M30 CD4+ cells. Average insert size: 1.0 kb; Uni-ZAP XR Vector; ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'

text: M30 CD4+ cells

text: SOLR (kanamycin resistant)

text: blood

text: Mus musculus

text: EcoRI

text: XhoI

text: Tcell

text: Stratagene mouse Tcell 937311

text: phagemid (ampicillin resistant)

text: pBluescript SK-


text: College of Biological Science, Beijing Agricultural University

text: live

text: Generic

text: Organism name synonym=Phaseolus aureus

text: E.coli DH5a

text: Vigna radiata

text: Mung Bean Hypocotyl

text: Plasmid


text: Albert Einstein College of Medicine

text: live

text: Generic

text: liver

text: Mus musculus

text: Mouse liver (A.Puech)


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Umbilical vein endothelial cells, passaged once. Average insert size: 1.0 kb; Uni-ZAP XR Vector; ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'

text: umbilical vein, 1 passage

text: SOLR (kanamycin resistant)

text: Homo sapiens

text: EcoRI

text: XhoI

text: Stratagene endothelial cell 937223

text: phagemid (ampicillin resistant)

text: pBluescript SK-


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. NT2 (Ntera-2/c1.D1) precursor cells induced with Retinoic Acid for 1 week, followed by 3 weeks in mitotic inhibitors (Replate #2). Average insert size: 1.1 kb; Uni-ZAP XR Vector; ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'

text: Ntera-2/RA+MI neuroepithelial cells

text: SOLR (kanamycin resistant)

text: Homo sapiens

text: EcoRI

text: XhoI

text: Stratagene neuroepithelium NT2RAMI 937234

text: phagemid (ampicillin resistant)

text: pBluescript SK-


text: School of Biochemistry & Genetics, University of Newcastle upon Tyne

text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Differentiated, post mitotic hNT neurons. Average insert size: 1.5 kb; Uni-ZAP XR Vector; ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'

text: hNT neurons

text: SOLR (kanamycin resistant)

text: Homo sapiens

text: EcoRI

text: XhoI

text: Stratagene hNT neuron (#937233)

text: phagemid (ampicillin resistant)

text: pBluescript SK-


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. 6-8 month old female lung and 1.5 year old male lung were source of mRNA. Average insert size: 1.5 kb; Uni-ZAP XR Vector; ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'

text: 6-8 month old

text: SOLR (kanamycin resistant)

text: lung

text: Mus musculus

text: EcoRI

text: XhoI

text: female

characteristic: sex

text: C57BL/6 x CBA

text: lung

text: Stratagene mouse lung 937302

text: phagemid (ampicillin resistant)

text: pBluescript SK-


text: live

characteristic: INSDC status

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. HeLa S3 epithelioid carcinoma cells grown to semi-confluency without induction. Average insert size: 1.5 kb; Uni-ZAP XR Vector. ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'

text: HeLa S3 cell line

text: SOLR (kanamycin resistant)

text: Homo sapiens

text: EcoRI

text: XhoI

text: female

text: Stratagene HeLa cell s3 937216

text: phagemid (ampicillin resistant)

text: pBluescript SK-


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

characteristic: NCBI submission model

text: Cloned unidirectionally. Primer: Oligo dT. 10.5dpc embryos. pCMV-SPORT2 vector.

text: 10.5dpc embryos

text: whole embryo

text: Mus musculus

text: SalI

text: NotI

text: embryo

text: Life Tech mouse embryo 10 5dpc 10665016

text: phagemid (ampicillin resistant)


text: live

text: Generic

characteristic: NCBI submission package

text: Cloned unidirectionally. Primer: Oligo dT. 15.5dpc embryos. pCMV-SPORT2 vector.

text: 15.5dpc embryos

text: whole embryo

text: Mus musculus

text: SalI

text: NotI

text: embryo

text: Life Tech mouse embryo 15 5dpc 10667012

text: phagemid (ampicillin resistant)


text: Washington University School of Medicine

text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Skeletal muscle from patient with malignant hyperthermia. Average insert size: 1.0 kb; Uni-ZAP XR Vector; ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'

characteristic: description

text: adult

text: SOLR (kanamycin resistant)

text: skeletal muscle

text: Homo sapiens

text: EcoRI

text: XhoI

text: muscle

text: Stratagene muscle 937209

text: phagemid (ampicillin resistant)

text: pBluescript SK-


text: University of Edinburgh

text: live

text: Generic

text: Lymphatic filarial nematode parasite of humans. mRNA was prepared from approximately 50 adult females isolated from the peritoneal cavity of jirds and converted to double-stranded cDNA using reverse transcriptase and oligo(dT) followed by RNase H and DNA pol I. The library has 5 x 10E6 independent recombinants and the average insert size is ~900bp.The library was constructed by Michelle Lizotte-Waniewski. The library is available from Dr.S.A.Williams, email: genome@smith.edu.

text: adult

text: XL1-Blue MRF'

text: Brugia malayi

text: Eco RI

text: Xho I

text: female

text: Brugia malayi adult female cDNA (SAW96MLW-BmAF)

text: Phage

text: Lambda Uni-ZAP XR


text: Washington University School of MedicineP

text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. 13.5dpc embryos. pCMV-SPORT2 vector.

text: 13.5dpc embryos

text: whole embryo

text: Mus musculus

text: SalI

text: NotI

characteristic: strain

text: embryo

text: Life Tech mouse embryo 13 5dpc 10666014

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: 23 year old

text: thymus

text: Homo sapiens

characteristic: organism

text: female

text: thymus

text: Human thymus (V.L.Boyartchuk)


text: live

text: Generic

text: fetal

characteristic: dev-stage

text: brain

text: Homo sapiens

text: brain

text: Human fetal brain (G.G.Consalez)


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Uninduced, exponentially growing neuroepithelial cells (Ntera-2/c1.D1). Average insert size: 1.0 kb; Uni-ZAP XR Vector; ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'

text: Ntera-2 neuroepithelial cells

text: SOLR (kanamycin resistant)

text: brain

characteristic: organ

text: Homo sapiens

text: EcoRI

text: XhoI

text: neuroepithelial cells

text: Stratagene NT2 neuronal precursor 937230

text: phagemid (ampicillin resistant)

text: pBluescript SK-


text: live

text: Generic

characteristic: cell-line

text: Cloned unidirectionally. Primer: Oligo dT. Small cell carcinoma cell line NCI-H69. Average insert size: 1.0 kb; Uni-ZAP XR Vector; ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'

text: cell line NCI-H69

text: SOLR (kanamycin resistant)

text: lung

text: Homo sapiens

text: EcoRI

text: XhoI

text: lung carcinoma

text: Stratagene lung carcinoma 937218

characteristic: title

text: phagemid (ampicillin resistant)

text: pBluescript SK-


characteristic: External Id

text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. 8.5dpc embryos. pCMV-SPORT2 vector.

text: 8.5dpc embryos

text: whole embryo

text: Mus musculus

text: SalI

text: NotI

text: embryo

text: Life Tech mouse embryo 8 5dpc 10664019

text: phagemid (ampicillin resistant)

characteristic: vector


characteristic: INSDC first public

text: live

text: Generic

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACGAATCTGAAGTGGGAGCGGCCGCCCTTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Eco RI adaptors [CATGGATTCGGTACC], digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library constructed by Bob Barstead.

text: 6 weeks

characteristic: lab-host

text: Mus musculus

text: EcoRI

text: NotI

text: mixed

text: BALB/c

text: Kidney

text: Barstead MPLRB1

text: phagemid (ampicillin resistant)


text: University of Washington Pathology Department

characteristic: INSDC center name

text: live

text: Generic

text: The library was constructed using polyA+ selected RNA reverse transcribed with oligo dT. This library is described in more detail in the following article. Cecilia Giacchelli, Nancy Bae, Donna Lombardi, Mark Majesky and Stephen Schwartz. 1991. Molecular cloning and characterization of 2B7, a rat mRNA which distinguishes smooth muscle cell phenotypes in vitro and is identical to osteopontin (secreted phosphoprotein I, 2aR). Biochem. Biophys. Res. Commun. 177, 867-873.

text: 12 day old

text: Aorta

text: Rattus norvegicus

text: Eco RI

text: Eco RI

text: Male

text: Wistar-Kyoto

text: Smooth muscle

characteristic: tissue-type

text: Phage

text: lamda zap II


characteristic: INSDC last update

text: live

text: Generic

text: The library was constructed by Dr. Joan Lemire at the laboratory of Dr. Stephen Schwartz at the University of Washington at Seattle. It was made using polyA+ selected RNA reverse transcribed with oligo dT.

text: three month old adult

text: aorta

text: Rattus norvegicus

text: Eco RI

text: Xho I

text: male

characteristic: sex

text: Wistar-Kyoto

text: smooth muscle

text: phage

text: lamda ZAP II


text: Institut National de la Sante et de la Recherche Medicale

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: postnatal day 24

text: Escherichia coli

text: cochlea

text: Rattus norvegicus

text: EcoRI

text: XhoI

text: Wistar

text: rat lambda ZAPII library (C.P.Hamel)

text: Phagemid

text: lambda ZAPII


text: live

text: Generic

characteristic: NCBI submission package

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCGTTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. RNA provided by Dr. Bertrand Jordan. Library went through two rounds of normalization, and was constructed by Bento Soares and M.Fatima Bonaldo.

characteristic: description

text: 4 weeks

text: Mus musculus

text: Not I

text: Eco RI

text: male

text: Thymus

text: phagemid (ampicillin resistant)


text: Institute of Basic Medical Sciences, Peking Union Medical College & Chinese Academy of Medical Sciences(PUMC & CAMS)

text: live

text: Generic

text: Splenic erythroblasts from FVA infected BALB/c mice were cultured with erythopoietin(EPO). Poly(A)RNA was isolated from both cells directly from spleens of FVA infected mice and cells after 36 hours culture with EPO. The differentially expressed cDNA fragments were cloned by substractive hybridization coupled with PCR amplification and sequenced by employing pBluescript SK as vector.

text: E.coli XL1-Blue

text: spleen

text: Mus musculus

characteristic: organism

text: erythroblast

text: Splenic erythroblasts from anemic strain of Friend virus infected mice

text: Phagemid

text: pBluescript SK


text: ICRF/UMDS

text: live

text: Generic

text: melanoma

characteristic: cell type

text: skin

text: Mus musculus

characteristic: strain

text: melanoma

text: mouse melanoma


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Gastrulating embryos were collected at 7.5dpc from C57BL6 x DBA matings, excluding embryos that had developed head folds and all extraembryonic tissues. Average insert size: 1.3 kb (range: 0.5 - 3.0 kb). Referenced in Development 121, 2479-2489 (1995)

characteristic: dev-stage

text: whole embryo

characteristic: organ

text: Mus musculus

text: SalI

text: NotI

text: pooled

text: C57BL/6 x DBA

text: embryo

text: Beddington mouse embryonic region

text: phagemid (ampicillin resistant)

text: pCMV-SPORT


characteristic: External Id

text: Washington University School of Medicine

text: live

text: Generic

text: 1st strand cDNA was primed with a XhoI - oligo(dT) primer. Double-stranded cDNA was ligated to EcoRI adaptors digested with XhoI and cloned into XhoI and EcoRI sites. Primary complexity of the library was 10 in the 7th. The library went through one round of amplification.

text: mixed stages (embryo to adult)

text: not applicable (host cell line)

text: Pristionchus pacificus

text: 5' EcoRI

text: 3' XhoI

text: predominantly hermaphroditic

text: PS 312

text: Sommer Pristionchus

characteristic: title

text: Phagemid (Ampicillin resistant)

text: Uni-ZAP XR Vector (Stratagene)


text: Shizuoka University

characteristic: INSDC first public

text: live

text: Generic

text: early globular

text: Citrus sinensis

text: Valencia

text: young seeds

text: Valencia orange young seeds cDNA


text: Shizuoka University

text: live

text: Generic

text: Miyagawa-wase satsuma mandarin

characteristic: cultivar

text: rapid developing stage

text: Citrus unshiu

text: juice sac and pulp segment

text: Miyagawa-wase satsuma mandarin orange (M.Omura)


text: Research Institute for Microbial Diseases, Osaka University

characteristic: INSDC center name

text: live

text: Generic

text: Mus musculus

text: male

text: ICR

text: testis

text: Mouse ICR testis (J.Tsuchida)


text: Department of Genetics, The Hospital for Sick Children

text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Pooled retinal tissue. Average insert size: 1.0 kb; Uni-ZAP XR Vector; ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'

text: SOLR (kanamycin resistant)

characteristic: lab-host

text: Homo sapiens

text: EcoRI

text: XhoI

text: mixed

text: Stratagene fetal retina 937202

text: phagemid (ampicillin resistant)

text: pBluescript SK-

characteristic: vector


text: Washington University School of MedicineP

characteristic: INSDC last update

text: live

text: Generic

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCGCTGTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. RNA provided by Dr. Bertrand Jordan. Library went through three rounds of normalization, and was constructed by Bento Soares and M.Fatima Bonaldo.

text: 4 weeks

text: Mus musculus

text: Not I

text: Eco RI

text: male

characteristic: sex

text: Spleen

characteristic: tissue-type

text: Soares mouse 3NbMS

text: phagemid (ampicillin resistant)


text: Queensland Institute of Medical Research

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Messenger RNA was isolated directly from lysates of schistosome miracidia using oligo-dT chromatography. First stand synthesis was primed with an oligo-dT-Xho-1 primer, and synthesized using M-MLV reverse transcriptase. Second strand synthesis was accomplished with RNaseH and T4 DNA polymerase. The double stranded cDNA was ligated to EcoR1 linkers, subsequently cleaved with EcoR1 and Xho1, and then ligated into phagemid lambda ZAP 11 XR

text: Miracidium

text: Mouse

text: Schistosoma japonicum

text: EcoR I

text: Xhol I

text: Mixed sex

text: Chinese (Anhui) strain

text: Whole organism

text: Miracidia Sjc 3/96

text: Phagemid

text: Lambda ZAP-II XR


text: Sidney Kimmel Cancer Center

text: live

text: Generic

text: melanocytes

characteristic: cell type

text: Clonetics Corp. (San Diego, CA) strain #68 and 2486

characteristic: cell-line

text: newborn

text: Homo sapiens

characteristic: organism

text: Human newborn melanocytes (T.Vogt)


text: Weizmann Institute of Science

text: live

text: Generic

text: Triticum aestivum

text: Triticum aestivum (G.Segal)


text: Centro de Pesquisas Rene Rachou, FIOCRUZ

text: live

text: Generic

text: mRNA was extracted from adult worm and the library was constructed according to the manufacturer's instructions (Uni-Zap XR vector, Stratagene)

characteristic: description

text: adult worm

characteristic: dev-stage

text: Schistosoma mansoni

text: EcoRI

text: XhoI

text: LE

characteristic: strain

text: Schistosoma mansoni, adult worm (J.C.Parra)

text: phage

text: Uni-ZAP XR vector


characteristic: External Id

text: Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais

characteristic: INSDC first public

text: live

text: Generic

text: mRNA was extracted from lung stage worms and the library was constructed according to the manufacturer's instructions (Uni-Zap XR vector, Stratagene)

text: Lung

text: Schistosoma mansoni

text: EcoRI

text: XhoI

text: NMRI

text: Schistosoma mansoni, lung stage

characteristic: title

text: lambda

text: Uni-ZAP XR vector


text: University of Berkeley

characteristic: INSDC center name

text: live

text: Generic

text: mRNA purified from rough endoplasmic reticulum-bound polysomes was used as a template. cDNA's directionally cloned at HinDIII and PstI in Bluescript SKt. Cloned into HinDIII, but the site was destroyed to add an adapter sequence. So ClaI is now the restriction enzyme at site I of vector. Primers - 5' universal, 3' m13-20 (reverse), 3' T3, 5' T7.

text: 0 to 24 hours old embryo

text: XL1 Blue MRF

characteristic: lab-host

text: embryo

characteristic: organ

text: Drosophila melanogaster

text: ClaI

text: PstI

text: male and female

text: Drosophila Embryonic Polysomal in pBS skT

text: plasmid


text: Institute of Medical and Veterinary Science

characteristic: INSDC last update

text: live

text: Generic

text: herpes simplex virus infected dorsal root ganglia

text: Mus musculus

text: dorsal root ganglia

text: Mouse herpes simplex virus infected (DTscharke)


text: Institut de Biologie Moleculair des Plantes du CNRS (IBMP)

text: live

text: Generic

text: equal quantities of 4 mRNA pools: 7 day old germinated etiolated seeds; tissue culture grown roots; staged plants with 24h light cycle, half on 16h light; and aerial tissues (stems, flowers and siliques) of plants

text: Arabidopsis thaliana

text: CO

text: Arabidopsis thaliana mRNA (J.Fleck)

characteristic: vector


text: live

characteristic: INSDC status

text: Generic

text: mRNA was extracted from eggs and the library was constructed and excised according to the manufacturer's instructions (Uni-Zap XR vector, Stratagene)

text: egg

text: Schistosoma mansoni

text: XhoI

text: EcoRI

text: NMRI

text: Schistosoma mansoni, egg stage

text: Phagemid

text: pBlueScript SK


text: Sidney Kimmel Cancer Center

text: live

text: Generic

characteristic: NCBI submission model

text: Mus musculus

text: cerebellum

characteristic: tissue-type

text: Mouse cerebellum library


text: University of Milan

text: live

text: Generic

characteristic: NCBI submission package

text: Homo sapiens

text: neuroblastoma

text: Human neuroblastoma SK-ER3 cells (M.Garnier)


text: live

text: Generic

text: mRNA was extracted from cercariae and the library was constructed and excised according to the manufacturer's instructions (Uni-Zap XR vector, Stratagene)

text: Larvae

text: Schistosoma mansoni

text: XhoI

text: EcoRI

text: NMRI

text: Schistosoma mansoni, cercariae

text: Phagemid

text: pBlueScript SK


text: International Institute of Genetics and Biophysics, CNR

text: live

text: Generic

text: Homo sapiens

text: thymus

text: Human thymus 610t (M.D'Urso)


text: Washington University School of MedicineP

text: live

text: Generic

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCGATACTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. RNA provided by Dr. Bertrand Jordan. Library constructed and normalized by Bento Soares and M.Fatima Bonaldo.

text: 4 weeks

text: lymph node

text: Mus musculus

text: Not I

text: Eco RI

text: male

characteristic: sex

text: lymph node

text: Soares mouse lymph node NbMLN

text: phagemid (ampicillin resistant)


text: University of Southern California

text: live

text: Generic

text: Mus musculus

characteristic: organism

text: Mouse (D.L.Crowe)


text: Univ California Irvine

text: live

text: Generic

text: asynchronous asexual blood stage

text: Plasmodium falciparum

text: Plasmodium falciparum NF54 (D.A.Fidock)


text: Sidney Kimmel Cancer Center

text: live

text: Generic

text: Mus musculus

text: cortex

text: Mouse cortex library


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: 1st strand cDNA was primed with oligo(dT)17 on 50 ng of DNAse-treated, total cellular RNA obtained from 5,000-10,000 microdissected, histologically normal prostate epithelial cells. Double-stranded cDNA was ligated to EcoRI adaptors, 5 cycles of PCR applied to the cDNA with an adaptor-specific primer, and the resulting PCR product subcloned into pAMP10 by the UDG-cloning method (Life Technologies). Average insert size is 600 bp. NOTE: Not directionally cloned. This library was constructed by David Krizman.

characteristic: description

text: 45 years old

text: Homo sapiens

text: EcoRI

text: Male

text: Phagemid (ampicillin resistant)


text: live

text: Generic

text: 1st strand cDNA was primed with oligo(dT)17 on 50 ng of DNAse-treated, total cellular RNA obtained from 5,000-10,000 microdissected preneoplastic cells histologically-determined to be prostatic intraepithelial neoplasia 2 (PIN2) cells. Double-stranded cDNA was ligated to EcoRI adaptors, 5 cycles of PCR applied to the cDNA with an adaptor-specific primer, and the resulting PCR product subcloned into pAMP10 by the UDG-cloning method (Life Technologies). Average insert size is 600 bp. NOTE: Not directionally cloned. This library was constructed by David Krizman.

text: 45 years old

characteristic: dev-stage

text: Homo sapiens

text: EcoRI

text: Male

text: Phagemid (ampicillin resistant)


text: live

text: Generic

text: 1st strand cDNA was primed with oligo(dT)17 on 50 ng of DNAse-treated, total cellular RNA obtained from 5,000-10,000 microdissected cells histologically-determined to be fully malignant prostate cancer cells. Double-stranded cDNA was ligated to EcoRI adaptors, 5 cycles of PCR applied to the cDNA with an adaptor-specific primer, and the resulting PCR product subcloned into pAMP10 by the UDG-cloning method (Life Technologies). Average insert size is 600 bp. NOTE: Not directionally cloned. This library was constructed by David Krizman.

text: 45 years old

text: Homo sapiens

text: EcoRI

text: Male

text: Phagemid (ampicillin resistant)


text: Jikei University School of Medicine

text: live

text: Generic

text: Trichoplusia ni

text: Cabbage looper mRNA


text: San Francisco General Hospital-University of California, San Francisco

text: live

text: Generic

text: Total RNA was isolated from purified Cryptosporidium parvum sporozoites using TRIZOL reagent (GIBCO-BRL). Directional cDNA was synthesized by first-strand priming with a Xho I-oligo d(T) linker-primer, second-stranding with RNase H and DNA polymerase I, ligation of EcoR I linkers, and digestion with Xho I, all using the Stratagene ZAP-cDNA synthesis kit. The cDNA was cloned into the EcoR I and Xho I sites of Lambda Uni-ZAP XR vector; the primary library was >97% recombinant and contained 1.3 X 10(6) independent clones with an ca. average insert size of 1.3 kb. Based on open reading frame (orf) analysis of the first 64 sequence tags we estimate that up to one-third of the library is composed of genomic DNA clones since approximately 15% of the orfs were incorrectly oriented on the antisense strand.

text: sporozoite

text: E. coli XL1 Blue MRF' Kan

text: Cryptosporidium parvum

text: EcoR I

text: Xho I

text: IOWA

characteristic: strain

text: UniZAP XR


characteristic: External Id

text: Forest Research Institute

text: live

text: Generic

text: pre-meiotic

text: E. coli, DH10B

text: male cones

text: Pinus radiata

text: cDNA library from pre-meiotic male cones

text: plasmid


text: Imperial Cancer Research Fund

characteristic: INSDC first public

text: live

text: Generic

text: keratinocyte

characteristic: cell type

text: keratinocyte cells cultured on mouse 3T3 feeder cells ; differentially expressed between basal cell carcinoma (BCC) cells and normal skin cells

text: Homo sapiens

text: Human keratinocyte differential display (B.Lin)


text: Sidney Kimmel Cancer Center

characteristic: INSDC center name

text: live

text: Generic

text: brain

text: Mus musculus

text: Mouse brain library

characteristic: title


text: AMC Cancer Research Center

text: live

text: Generic

text: Rattus norvegicus

text: Sprague-Dawley

text: Rat (J.X.Lu)


text: Berkeley Laboratory

text: live

text: Generic

text: infant

text: brain

characteristic: organ

text: Homo sapiens

text: Human infant brain (J.-F.Cheng)


text: Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais

characteristic: INSDC last update

text: live

text: Generic

text: mRNA was extracted from adult worms and the library was constructed and excised according to the manufacturer's instructions (Uni-Zap XR vector, Stratagene)

text: adult

characteristic: lab-host

text: Schistosoma mansoni

text: XhoI

text: EcoRI

text: Schistosoma mansoni, adult worm (Pena,H.B. and Pena,S.D.J.)

text: Phagemid

text: pBlueScript SK


text: University of Edinburgh

text: live

characteristic: INSDC status

text: Generic

text: Meloidogyne javanica

text: Meloidogyne javanica mRNA


text: Institute of Radiation Medicine

text: live

text: Generic

characteristic: NCBI submission model

text: epithelial

text: Rattus norvegicus

text: Wistar

text: trachea

text: Rat trachea (Gang Li)


text: University of Cincinnati Medical Center

text: live

text: Generic

text: colonic mucosa cells

text: cDNAs were obtained by using mRNA differential display technique from the mucosa cells of ulcerative colitis.

text: Homo sapiens

text: colonic mucosa

characteristic: tissue-type

text: Human Ulcerative Colitis Mucosa Express Library


text: Memorial University of Newfoundland

text: live

text: Generic

characteristic: NCBI submission package

text: Salmo salar

text: muscle

text: Atlantic salmon muscle (C.McGowan)


text: Memorial University of Newfoundland

text: live

text: Generic

text: liver

text: Salmo salar

text: Atlantic salmon liver (C.McGowan)


text: live

text: Generic

text: Equal amounts of plasmid DNA from three normalized libraries (melanocyte 2NbHM, pregnant uterus NbHPU, and fetal heart NbHH19W) were mixed, and ss circles were made in vitro. Following HAP purification, this DNA was used as tracer in a subtractive hybridization reaction. The driver was PCR-amplified cDNAs from pools of 5,000 clones made from the same 3 libraries. The pools consisted of I.M.A.G.E. clones 260232-265223, 340488-345479, and 484488-489479.

text: mixed (see below)

text: Homo sapiens

text: Not I

text: Eco RI

text: Pooled human melanocyte, fetal heart, and pregnant uterus

text: phagemid (ampicillin resistant)

characteristic: vector


text: Harvard Medical School

text: live

text: Generic

text: No library was made. cDNA from individual Leishmania major lines containing random transposon insertions that activated a selectable mariner element (MosHYG) was amplified by PCR. PCR primers corresponded to the mini-exon sequence present in the 5' end of every trypanosomatid mRNA (forward primer) and to MosHYG sequences (reverse primer). PCR products were cloned in the pGEM-T vector and sequenced using SP6/T7 primers.

text: promastigote

text: Leishmania major

characteristic: organism

text: Random insertions of transposon MosHYG in Leishmania major


text: Beijing Medical University

text: live

text: Generic

text: GM-CSF-deprived TF-1 cell line

characteristic: cell-line

text: Homo sapiens

text: Human GM-CSF-deprived TF-1 cell line (Liu,Hongtao)


text: Washington University School of Medicine

text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size: 1.0 kb; Uni-ZAP XR Vector; ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3' Library provided Lisa Guay-Woodford.

characteristic: description

text: newborn (day 0)

characteristic: dev-stage

text: SOLR (kanamycin resistant)

text: kidney

text: Mus musculus

text: EcoRI

text: XhoI

text: kidney

text: GuayWoodford Beier mouse kidney day 0

text: phagemid (ampicillin resistant)

text: pBluescript SK-


characteristic: External Id

text: Washington University School of MedicineP

text: live

text: Generic

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCCTTATTTTTTTTTTTTTTTTTT 3'], on total mouse RNA [provided by Minoru Ko, Wayne State Univ.]; double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library went through one round of normalization, and was constructed by Bento Soares and M. Fatima Bonaldo.

text: 12.5dpc total fetus

text: whole fetus

text: Mus musculus

text: Not I

text: Eco RI

characteristic: strain

text: fetus

text: Soares mouse 3NME12 5

text: phagemid (ampicillin resistant)


text: Lawrence Berkeley National Lab

characteristic: INSDC first public

text: live

text: Generic

text: Constructed using Stratagene ZAP-cDNA Synthesis kit. Oligo dT-primed and directionally cloned at EcoRI and XhoI in BlueScript SK(+/-)

text: 0 to 24 hours mixed stage embryonic

text: XL1 Blue MRF

text: embryo

text: Drosophila melanogaster

text: EcoRI

text: XhoI

text: male and female

characteristic: sex

text: LD Drosophila Embryo

text: BlueScript SK


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

characteristic: INSDC center name

text: live

text: Generic

text: 1st strand cDNA was prepared from human tonsillar cells enriched for germinal center B cells by flow sorting (CD20+, IgD-), provided by Dr. Louis M. Staudt (NCI), Dr. David Allman (NCI) and Dr. Gerald Marti (CBER). cDNA synthesis was primed with a Not I - oligo(dT) primer [5'-TGTTACCAATCTGAAGTGGGAGCGGCCGCCTCATTTTTTTTTTTTTTTTTT-3']. Double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library went through one round of normalization, and was constructed by Bento Soares and M. Fatima Bonaldo.

text: Homo sapiens

text: Not I

text: Eco RI

text: germinal center B cell

characteristic: title

text: phagemid (ampicillin resistant)


characteristic: INSDC last update

text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size: 1.0 kb; Uni-ZAP XR Vector; ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3' Library provided by Lisa Guay-Woodford.

text: juvenile (7 days old)

text: SOLR (kanamycin resistant)

text: kidney

characteristic: organ

text: Mus musculus

text: EcoRI

text: XhoI

text: kidney

text: GuayWoodford Beier mouse kidney day 7

text: phagemid (ampicillin resistant)

text: pBluescript SK-


text: Children's Memorial Research Center

text: live

characteristic: INSDC status

text: Generic

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' AACTGGAAGAATTCGCGGCCGCAGGAATTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library went through a reassociation kinetics procedure to generate a mini-library (Nb2HP-B) enriched for mRNAs of the prevalent and intermediate frequency classes. Library constructed by Bento Soares and M.Fatima Bonaldo.

text: placenta obtained at birth (full term)

text: DH10B (ampicillin resistant)

characteristic: lab-host

text: Homo sapiens

text: Not I

text: Eco RI

text: Female

text: Soares placenta Nb2HP-B

text: phagemid

text: pT7T3D (Pharmacia) with a modified polylinker


text: live

text: Generic

characteristic: NCBI submission model

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCGAATCTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library constructed and normalized by Bento Soares and M.Fatima Bonaldo.

text: Mus musculus

text: Not I

text: Eco RI

text: Liver

characteristic: tissue-type

text: Soares mouse NML

text: phagemid (ampicillin resistant)


text: The Toronto Hospital

text: live

text: Generic

characteristic: NCBI submission package

text: promyeloblast

characteristic: cell type

text: Unidirectional cloning sites: EcoR1-Xho1. mRNA was purified from KG1-a cell line, cDNA was synthesized using an Xho1-OligodT linker primer. EcoR1 adaptors were ligated, followed by digestion with Xho1 for directional cloning into predigested Lambda Zap Express

text: Homo sapiens

text: EcoRI

text: XhoI

text: KG1-a Lambda Zap Express cDNA library

text: Lambda Zap Express (Stratagene)

characteristic: vector


text: National Institutes of Health

text: live

text: Generic

text: melanoma UACC-903

characteristic: cell-line

text: Homo sapiens

characteristic: organism

text: Human microdissection mediated cDNA capture clones


characteristic: External Id

text: Beijing Medical University

text: live

text: Generic

text: Homo sapiens

text: Human cell line TF-1 (D.L.Ma)


text: Forest Research Institute

text: live

text: Generic

text: embryogenic

text: A cDNA library was made from somatic embryogenic tissue containing pre-bullet stage embryos; Pinus radiata D Don

characteristic: description

text: pre-bullet

characteristic: dev-stage

text: E.coli DH10B

text: Pinus radiata

text: embryogenic tissue

text: Pinus radiata somatic embryogenic library

text: Plasmid


text: University of Edinburgh

characteristic: INSDC first public

text: live

text: Generic

text: Brugia malayi is a lymphatic filarial nematode parasite of humans. Full length cDNA was prepared by long-range RT-PCR from mRNA from L2 larvae of the human filarial nematode parasite Brugia malayi using nematode spliced leader (SL, 5'end) and oligo-d(T) (3' end) primers. The library had an unamplified titre of ~1 x 10E6 per ml and ~95% of clones have inserts (mean length ~900 bp). The library is available from The Filarial Genome Project Resource Center: contact Dr. S.A. Williams, Clark Science Center, Smith College, Northampton, MA 01063 USA phone +1 413 585 3826 fax +1 413 585 3786 email genome@smith.edu

text: mosquito derived, second stage larvae (L2)

text: E. coli XL1-Blue MRF-

text: Brugia malayi

text: Eco R I (5' end)

text: Xho I (3' end)

text: mixed

characteristic: sex

text: TRS Labs

characteristic: strain

text: Brugia malayi second stage larvae JHU96SL-BmL2

text: Phage

text: lambdaZapII


characteristic: INSDC center name

characteristic: INSDC last update

text: live

text: Generic

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACGAATCTGAAGTGGGAGCGGCCGCCCTTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Eco RI adaptors [GATGGATTCGGTACC], digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library constructed by Bob Barstead.

text: 6 weeks

text: lung

text: Mus musculus

text: EcoRI

text: NotI

text: mixed

text: BALB/c

text: lung

text: Barstead mouse lung MPLRB2

characteristic: title

text: phagemid (ampicillin resistant)


text: Sidney Kimmel Cancer Center

text: live

characteristic: INSDC status

text: Generic

text: Mus musculus

text: spinal chord

text: Mouse spinal chord library


text: Duke University

text: live

text: Generic

characteristic: NCBI submission model

text: Drosophila melanogaster

text: Drosophila melanogaster (R.G.Fehon)


text: Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais

text: live

text: Generic

text: mRNA was extracted from adult worms and the library was constructed according to the manufacturer's instructions (Uni-Zap XR vector, Stratagene)

text: adult

characteristic: lab-host

text: Schistosoma mansoni

text: XhoI

text: EcoRI

text: NMRI

text: Schistosoma mansoni, adult worm (Rabelo, E. and Pena S.D.J.)

text: lambda

text: Uni-ZAP XR vector


text: Washington University School of MedicineP

text: live

text: Generic

characteristic: NCBI submission package

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACGAATCTGAAGTGGGAGCGGCCGCCCTTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Eco RI adaptors [GTTGGATTCGGTACC], digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library constructed by Bob Barstead.

text: 7 day

text: pooled

characteristic: organ

text: Mus musculus

text: EcoRI

text: NotI

text: mixed

text: FVB/N

text: pooled organs

characteristic: tissue-type

text: Barstead mouse pooled organs MPLRB4

text: phagemid (ampicillin resistant)


text: live

text: Generic

characteristic: cultivar

text: A library of 3,000,000 clones was constructed using mRNA harvested from male cones picked 1.5 months prior to meiosis. Fourteen randomly selected clones had inserts ranging in size from 500 bp to 2 kb.

text: pre meiotic

text: Escherichia coli

text: male cone

text: Pinus radiata

text: Not I

text: Sal I

text: Pinus radiata pre meiotic male cone

text: plasmid

characteristic: vector


characteristic: External Id

text: New Zealand Forest Research Institute

text: live

text: Generic

text: A library of 6,000,000 clones was constructed using mRNA harvested from male cones picked one month after meiosis. Eighteen randomly selected clones had inserts ranging in size from 500 bp. to 2 kbp.

text: post meiotic

text: Escherichia coli

text: male cone

text: Pinus radiata

characteristic: organism

text: Not I

text: Sal I

text: Pinus radiata post meiotic male cone

text: plasmid


text: INSERM U-393

text: live

text: Generic

text: lymphoblastoid

characteristic: cell type

text: from RT-PCR amplification

characteristic: description

text: Homo sapiens

text: Human RT-PCR (S.Lefebvre)


text: Washington University School of Medicine

characteristic: INSDC first public

text: live

text: Generic

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACGAATCTGAAGTGGGAGCGGCCGCCCTTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Eco RI adaptors [CTTGGATTCGGTACC], digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library constructed by Bob Barstead.

text: 6 weeks

characteristic: dev-stage

text: heart

text: Mus musculus

text: EcoRI

text: NotI

text: mixed

characteristic: sex

text: BALB/c

text: heart

text: Barstead mouse heart MPLRB3

text: phagemid (ampicillin resistant)


text: San Francisco General Hospital-University of California, San Francisco

characteristic: INSDC center name

characteristic: INSDC last update

text: live

text: Generic

text: Total RNA was isolated from purified Cryptosporidium parvum sporozoites using TRIZOL reagent (GIBCO-BRL), and contaminating DNA was removed by treatment with RNase-free Dnase I. Poly A+ RNA was prepared using the Promega Poly A Tract kit and directional cDNA was synthesized by first-strand priming with a Xho I-oligo d(T) linker-primer, second-stranding with RNase H and DNA polymerase I, ligation of EcoR I-Not I linkers, and digestion with Xho I. The cDNA was cloned into the EcoR I and Xho I sites of pBlueScript II (SK minus) plasmid vector (Stratagene), and transformed into XL2 Blue ultracompetent bacteria. The primary library was 97% recombinant and contained approximately 1 X 10(5) independent clones. PCR analysis of 20 random clones indicated that the average insert size was ca. 1.0 kb.

text: sporozoite

text: E. coli XL2 Blue

text: Cryptosporidium parvum

text: EcoR I

text: Xho I

text: IOWA

characteristic: strain

text: pBlueScript II (SK minus)


text: Juntendo University School of Medicine

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Oryctolagus cuniculus

text: Rabbit mRNA (Y.Hotta)

characteristic: title


text: University of Tsukuba

text: live

text: Generic

characteristic: NCBI submission package

text: sexually competent

text: Dictyostelium discoideum

text: Dictyostelium discoideum sexually competent (H.Urushihara)


text: Zhejiang Medical University

text: live

text: Generic

text: taxol-induced apoptotic cells

text: Homo sapiens

text: Human breast cancer cell line Bcap 37


text: New Zealand Forest Research Institute

text: live

text: Generic

text: A cDNA library was cloned from male cones of Pinus radiata D Don

text: E.coli DH10B

characteristic: lab-host

text: Pinus radiata

text: Male cones

characteristic: tissue-type

text: Pinus radiata male cone library

text: Plasmid


text: Cornell University

text: live

text: Generic

text: Willis

characteristic: cultivar

text: A Lambda ZAP II cDNA library was constructed from mRNA extracted from etiolated leaf tissue of the barley cultivar 'Willis' and converted to pBluescript (amp resistant) as described in Heun et al. (1991) Genome 34:437-447. For insert amplification, Use M13 forward and reverse primers. Clones from this library are designated with the prefix 'BCD'.

text: Hordeum vulgare subsp. vulgare

text: EcoRI

text: etiolated leaf

text: cDNA from barley

text: Phage/Phagemid

text: Lambda Zap II/pBluescript

characteristic: vector


characteristic: External Id

text: Cornell University

text: live

text: Generic

text: Brooks

text: A Uni-ZAP XR cDNA library was constructed from etiolated leaf mRNA from the oat cultivar 'Brooks' and converted to pBluescript (amp resistant) as described in Heun et al. (1991) Genome 34:437-447. For insert amplification, use M13 forward and reverse primers. Clones from this library are designated with the prefix 'CDO'. *Note: Clone CDO1081 was recloned into the TA cloning vector and carries kanamycin resistance.

text: Avena sativa

characteristic: organism

text: EcoRI

text: XhoI

text: etiolated leaf

text: cDNA from oat

text: Phage/Phagemid

text: Uni-ZAP XR/pBluescript


text: Agriculture and Agri-food Canada

text: live

text: Generic

text: Chinese Spring

text: A pGEM-4Z (amp resistant) cDNA library was constructed from mRNA extracted from etiolated leaf tissue of the wheat cultivar 'Chinese Spring' as described in Heun et al. (1991) Genome 34:437-447. For insert amplification, Use M13 forward and reverse primers. Clones from this library are designated with the prefix 'WG'.

characteristic: description

text: Triticum aestivum

text: PstI

text: PstI

text: etiolated leaf

text: Genomic clones from wheat

text: plasmid


text: Faculty of Science, Ain Shams University

characteristic: INSDC first public

text: live

text: Generic

text: Sporocyst

text: Schistosoma mansoni

text: NMRI

text: Schistosoma mansoni Lambda Gt11 (Mohamed MM)

text: phage

text: lambda gt11


text: University of Texas Southwestern Medical Center at Dallas

text: live

text: Generic

text: HeLa

characteristic: cell-line

text: Homo sapiens

text: Human HeLa cells (M.Lovett)


text: live

text: Generic

text: fetal

characteristic: dev-stage

text: Homo sapiens

text: brain

text: Human fetal brain (M.Lovett)


characteristic: INSDC center name

text: live

text: Generic

text: Homo sapiens

text: Human (M.Lovett)


text: Sidney Kimmel Cancer Center

characteristic: INSDC last update

text: live

text: Generic

text: Mus musculus

text: forebrain

text: Mouse forebrain library


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: 1st strand cDNA was prepared from mRNA obtained from Clontech Laboratories, Inc., and primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCCCAATTTTTTTTTTTTTTTTT 3']. Double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library went through one round of normalization to Cot5, and was constructed by Bento Soares and M. Fatima Bonaldo.

text: Homo sapiens

text: Not I

text: Eco RI

text: male

characteristic: sex

text: phagemid (ampicillin resistant)


text: live

characteristic: INSDC status

text: Generic

text: Library constructed from S-11 frontal lobe, male, 34 years old, 50% caucasian, 50% Aleutian. Schizophrenic suicide. Random primed into EcoRI site of ZAP II Vector. Mass excised. Custom library. Avg insert length 1.4kb. Material obtained by Johnston N., Torrey, E.F., Yolken R., and the Stanley Neuropathology Consortium - Analysis of RNAs from the Brains of Individuals with Psychiatric Diseases (Unpublished) Stanley Neurovirology Laboratory, Johns Hopkins School of Medicine, Baltimore MD.

text: 34 years old

text: SOLR (kanamycin resistant)

text: Homo sapiens

text: EcoRI

text: male

text: schizophrenic brain S-11 frontal lobe

text: Stratagene schizo brain S11

text: phagemid (ampicillin resistant)

text: Bluescript SK-


text: The Johns Hopkins University School of Medicine

text: live

text: Generic

characteristic: NCBI submission model

text: Homo sapiens

text: retina

text: Human retina (D.Swanson)


text: Universitaet Tuebingen

text: live

text: Generic

text: Amanita muscaria

text: Amanita muscaria mRNA

characteristic: title


text: University of California, San Diego

text: live

text: Generic

characteristic: NCBI submission package

text: E2a-Pbx1 induced fibroblasts

characteristic: cell type

text: cell line

characteristic: organ

text: Mus musculus

text: fibroblasts

text: Mouse NIH3T3 (X.Fu)


text: Rockefeller University

text: live

text: Generic

text: Mus musculus

text: Mouse mRNA (N.Heintz)


text: Ehime University School of Medicine

text: live

text: Generic

text: lymphokine activated killer cells

text: LAK

text: Homo sapiens

text: male

text: Human LAK cell (Y.Abe)


text: Humboldt-University

text: live

text: Generic

text: Haisa

characteristic: cultivar

text: Hordeum vulgare subsp. vulgare

text: primary leaf

characteristic: tissue-type

text: Barley cultivar Haisa leaf (W.R.Hess)

text: phage

text: lambda gt11


characteristic: External Id

text: The University of Michigan Medical School

text: live

text: Generic

text: Adult pituitary cDNA was used for cDNA selection with P1 clones from the df region of mouse chromosome 11 (MMU11). The selected cDNAs were cloned into the SmaI site of pGEM-3z. ESTs were confirmed by hybridization and PCR to map to MMU11 between D11Mit153 and D11Mit154.

text: adult

characteristic: lab-host

text: pituitary

text: Mus musculus

text: mix of male and female

text: mix of various genetic backgrounds

characteristic: strain

text: cDNA selection products from df region of MMU11

text: Plasmid

characteristic: vector


characteristic: INSDC first public

text: live

text: Generic

text: ES

characteristic: cell-line

text: Exon amplification was carried out on P1 clones from the df region of mouse chromosome 11 (MMU11). The resulting exon clones were confirmed by hybridization and PCR to map to MMU11 between D11Mit153 and D11Mit154.

characteristic: description

text: Mus musculus

characteristic: organism

text: exon amplification products from df region of MMU11

text: Plasmid


text: Hospital Vall Hebron

characteristic: INSDC center name

characteristic: INSDC last update

text: live

text: Generic

text: Mus musculus

text: Mouse mRNA (A.Meseguer)


text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Library provided by Dr. M. Saber TBRI, Cairo, Egypt

text: adult

characteristic: dev-stage

text: Schistosoma mansoni

text: mixed

characteristic: sex

text: S. mansoni adult Lambda Zap


text: live

text: Generic

characteristic: NCBI submission package

text: adult

text: Schistosoma mansoni

text: mixed

text: S. mansoni adult Lambda gt11

characteristic: title

text: Lambda gt11


text: live

text: Generic

text: Library Provided by Dr. Philip LoVerde, SUNY, Buffalo

text: cercarial

text: Schistosoma mansoni

text: mixed

text: S. mansoni cercarial Lambda gt11

text: Lambda gt11


text: live

text: Generic

text: schistosomule

text: Schistosoma mansoni

text: mixed

text: S. mansoni Schistosomule Lambda Zap

text: Lambda ZAP


text: live

text: Generic

text: Library provided by Dr. Jim McKerrow , UCSF

text: sporocyst

text: Schistosoma mansoni

text: mixed

text: S. mansoni sporocyst Lambda gt11


text: Washington University School of Medicine

text: live

text: Generic

text: Toxoplasma gondii parasites were grown in vitro in cultures of human foreskin fibroblasts under conditions that induce tachyzoite-bradyzoite differentiation. The library was constructed by A. Hehl and I. Manger, Stanford University. Directional cDNA was synthesized by oligo d(T) priming and cloned into EcoRI and XhoI sites of the Lambda ZAP vector using the ZAP-cDNA Synthesis Kit (Stratagene). WARNING: Library contains A SMALL PERCENTAGE OF human cDNAs derived from the host cells

text: in vitro Bradyzoite, 6day

text: XL 1-Blue MRF'

text: Toxoplasma gondii

text: EcoRI

text: NotI

text: TgME49 invivo Bradyzoite cDNA

text: phagemid

text: Lambda ZAP


text: Baylor Collge of Medicine

text: live

text: Generic

text: placental cDNA library, construction by Cheng Chi Lee

text: Homo sapiens

text: placenta

characteristic: tissue-type

text: Human placental cDNA (Cheng Chi Lee)


characteristic: External Id

text: University of Edinburgh

text: live

text: Generic

text: The cDNA library was constructed by Cindy Tripp (Heska Corporation, 1825 Sharp Point Drive, Fort Collins, Colorado 80525, USA) From 200,000 larval stage T. canis, using a single step guanadine-phenol-chloroform extraction, 265 ug total RNA was recovered, from which 6 ug poly-A+ RNA was isolated by oligo-dT chromatography. cDNA synthesised from this mRNA was unidirectionally cloned into the Uni-Zap XR phage vector, using packaging extracts from Stratagene. The amplified library contained 1.9 x 109 phage/ml with 91% recombinants.

text: Toxocara canis

text: Toxocara canis infective larva cDNA library