text: Northwest A&F University

text: live

text: Generic

text: Not I-oligo (dT) primed cDNA

characteristic: lab-host

text: mammary gland

text: Capra hircus

text: Xinong Saanen

characteristic: strain

text: Xinong Saanen dairy goat mammary


status: PUBLIC

characteristic: External Id

text: Warsaw Agricultural University

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: RNA was isolated using Chomczynski method with Trizol reagent. 100 micrograms of RNA was used to amplification using primers designed to LEC1 sequence from GenBank. The RT-PCR product was cloned into the TOPO vector and transformed the recombinant vector into competent E. coli.

characteristic: description

text: immature embryos

tag: attribute

characteristic: organ

text: Secale cereale

characteristic: organism

text: Inbred line L318

text: LEC1 Inbred line L318 TOPO TA Cloning

characteristic: title

text: pUC plasmid

text: PCRII-TOPO

characteristic: vector


text: Woods Hole Oceanographic Institution

text: live

text: Generic

text: Genes potentially upregulated in shallow, active C5 copepodids relative to deep, diapausing C5 copepodids.

text: Whole animal

text: Calanus finmarchicus

text: Immature, mixed sexes

characteristic: sex

text: C. finmarchicus C5 subtractive library, shallow


text: Woods Hole Oceanographic Institution

text: live

text: Generic

text: Genes potentially upregulated in deep, diapausing C5 copepodids relative to shallow, active C5 copepodids.

text: Whole animal

text: Calanus finmarchicus

text: Immature, mixed sexes

text: C. finmarchicus C5 subtractive library, deep


text: Solomon Park Research Institute

text: live

text: Generic

text: Murine spinal cord cDNA subtracted against white matter from cerebral cortex using suppression subtractive hybridization.

text: adult (90-110 days)

characteristic: dev-stage

text: spinal cord

text: Mus musculus

text: male

text: nervous

characteristic: tissue-type

text: Subtracted murine spinal cord minus white matter


text: UMR INRA PMS 1191

text: live

text: Generic

text: Parragio

characteristic: cultivar

text: A cDNA library of genes specifically expressed in embryo axis has been generated by a PCR-based suppressive subtractive hybridization (SSH) technique between mRNAs of two populations of embryo axis correspondent to two imbibition times 6 and 23 hours

text: germination

text: embryo axis

text: Medicago truncatula

text: Medicago truncatula SSH 6 Hours

text: Plasmid

text: pGEMT


text: ICAT, FCUL

text: live

text: Generic

text: Nugget

text: cDNA library was constructed using ZAP Express cDNA synthesis and ZAP Express cDNA Gigapack III Gold cloning kits (Stratagene, La Jolla, CA, USA)

text: E.coli

characteristic: lab-host

text: Internodes, prenodules, organogenic nodules

text: Humulus lupulus

text: Hop morphogenesis cDNA library


characteristic: External Id

text: University of Parma, Department of Environmental Sciences

text: live

text: Generic

text: Desiree

text: Primer employed for retrotranscription: T12VG; primer use for PCR: T12VG/AGCCAGCGAA. Sequences isolated by Differential Display and RT-PCR.

text: E.coli DH5alpha

text: Potato tubers with sprouts 2 mm long

text: Solanum tuberosum

text: Solanum tuberosum Sprouting tubers

text: pGEM-T


text: Shiraz University

characteristic: INSDC center name

text: live

text: Generic

text: Seedlings of Sardari, an Iranian wheat produced by dry farming, were grown in growth chamber for 10 days in hydroponics culture. Drought stress was induced by poly-ethylene glycol. Total mRNA isolation and cDNA synthesis were carried out from intact seedlings. Gene isolated by specific primers via PCR.

text: 10 days seedlings under drought stress

text: shoot and root

text: Triticum aestivum

text: Sardari

characteristic: strain

text: Triticum aestivum Sardari 10 days seedlings under drought stress


text: USDA-ARS and the University of Georgia

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Tiftrunner and GT-C20

text: Six cDNA libraries from R5,R6,R7 reproduction stages of developing seeds challenged by drought stress and Aspergillus parasiticus; two cultivars: Tiftrunner, susceptible to Aspergillus and resistant to TSWV, and GT-C20, susceptible to TSWV and reduced aflatoxin contamination.

text: R5, R6, R7

text: Developing seeds

text: Arachis hypogaea

text: EcoRI

text: XhoI

text: seed

text: Developing peanut seeds

text: Plasmid

text: pBluescript


text: Dept. Biology and C.R.I.B.I.-Biotechnology Centre, University of Padova

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Total RNA extracted from photophores (pool of 5 samples) was used to to make 3'-end cDNA library using a combination of SMART protocol (Clontech), ensuring almost full-length cDNA, and Gateway technology (Invitrogen), allowing unidirectional cloning without enzymatic digestion.

text: E.coli DH10B

text: Photophores

text: Euphausia superba

text: pool of males and females

text: pDONR221 (Invitrogen)


text: National Institute of Allergy and Infectious Diseases

text: live

text: Generic

characteristic: NCBI submission package

text: Secretory

characteristic: cell type

text: PCR-based cDNA library made with SMART cDNA library construction kit from Clontech

characteristic: description

text: Salivary gland

text: Ornithodoros parkeri

text: Salivary gland

text: Ornithodoros parkeri salivary gland OPSG-1

text: Phage

text: Lambda TriplEx2


text: Warsaw Agricultural University

text: live

text: Generic

text: RNA was isolated using Chomczynski method with Trizol reagent. 100 micrograms of RNA was used to amplification using primers designed to Zea mays viviparus 1 sequence from GenBank. The RT-PCR product was cloned into the TOPO vector and transformed the recombinant vector into competent E. coli.

text: immature embryos

characteristic: organ

text: Secale cereale

characteristic: organism

text: Inbred line L318

text: Secale cereale viviparus 1 TOPO TA Cloning

characteristic: title

text: pUC plasmid

text: PCRII-TOPO


text: University of Cambridge

text: live

text: Generic

text: Amplicon Express mixed pupal wing library (2) constructed on 9 August 2006 by Sandra Kazuko

text: Pupae

characteristic: dev-stage

text: pupal wings

text: Heliconius melpomene

text: cythera (UK bred)


text: live

text: Generic

text: Secretory

text: Salivary gland

text: Triatoma infestans

text: Salivary gland

characteristic: tissue-type

text: Triatoma infestans salivary gland cDNA library TISGL-1

text: Phage

text: Lambda TriplEx2

characteristic: vector


text: Beijing University of Agriculture

text: live

text: Generic

text: Total RNA isolated from Pseudosasa amabilis inflorescence by using the TRIzol reagent (GIBCOBRL). The cDNA library was constructed using the Creator SMART cDNA Library Construction Kit (CLONTECH).

text: E.coli. DH5a

characteristic: lab-host

text: Inflorescence

text: Pseudosasa amabilis

text: Sfi IA (GGCCATTACGGCC)

text: Sfi IB (GGCCGCCTCGGCC)

text: Inflorescence

text: Pseudosasa amabilis inflorescence cDNA library

text: Plasmid

text: pDNR-LIB


characteristic: External Id

text: USDA/ARS, Crop Protection and Management Research Unit

text: live

text: Generic

characteristic: cultivar

text: cDNA library was constructed from peanut cultivar Tifrunner (TF) or GT-C20 (C20). Tifrunner is resistant to TSMV and susceptible to Aspergillus parasiticus. GT-C20 is susceptible to TSMV, and has reduced aflatoxin contamination. Developing seeds were challenged by drought stress and Aspergillus, and collected at reproduction stages, R5, R6, or R7. Total RNA was isolated with RNeasy Kit (QIAGEN). mRNA was extracted from total RNA using Oligotex (QIAGEN). cDNA synthesis and library construction followed the protocol of ZAP Express cDNA Synthesis Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit (Stratagene). The cDNA above 300 bp was collected after size-fraction. The inserts were directionally cloned into ZAP Express vector using XhoI and EcoRI adapters. The lambda library was packed into phage using Gigapack III Gold (Stratagene). The unamplified library was used to excise pBluescript phagemids from the ZAP Express vector, and the phagemids were used to transform the host bacteria XL1-Blue MRF cells.

text: XL1-Blue MRF

text: Developing Seeds

text: Arachis hypogaea

text: EcoRI

text: XhoI

text: Seed

text: USDA-Tifton Peanut Library C20R5

text: Plasmid

text: pBlueScriptIISK+


characteristic: INSDC center name

text: live

text: Generic

text: XL1-Blue MRF

text: Developing Seeds

text: Arachis hypogaea

text: EcoRI

text: XhoI

text: Seed

text: USDA-Tifton Peanut Library C20R6

text: Plasmid

text: pBlueScriptIISK+


characteristic: INSDC first public

text: live

text: Generic

text: XL1-Blue MRF

text: Developing Seeds

text: Arachis hypogaea

text: EcoRI

text: XhoI

text: Seed

text: USDA-Tifton Peanut Library C20R7

text: Plasmid

text: pBlueScriptIISK+


characteristic: INSDC last update

text: live

text: Generic

text: Tifrunner

text: XL1-Blue MRF

text: Developing Seeds

text: Arachis hypogaea

text: EcoRI

text: XhoI

text: Seed

text: USDA-Tifton Peanut Library TFR5

text: Plasmid

text: pBlueScriptIISK+


text: live

characteristic: INSDC status

text: Generic

text: Tifrunner

text: XL1-Blue MRF

text: Developing Seeds

text: Arachis hypogaea

text: EcoRI

text: XhoI

text: Seed

text: USDA-Tifton Peanut Library TFR6

text: Plasmid

text: pBlueScriptIISK+


text: live

text: Generic

characteristic: NCBI submission model

text: Tifrunner

text: XL1-Blue MRF

text: Developing Seeds

text: Arachis hypogaea

text: EcoRI

text: XhoI

text: Seed

text: USDA-Tifton Peanut Library TFR7

text: Plasmid

text: pBlueScriptIISK+


text: University of Georgia

text: live

text: Generic

characteristic: NCBI submission package

text: The library was prepared from polyA+ RNA from premeiotic flowerbuds. Only floral buds with diameter of 2.5 mm of less were used for RNA isolation. Double-stranded cDNA was unidirectionally cloned into pBluescript SK- (Stratagene) with the 5' end of the cDNA inserted at the EcoRI site and the 3' polyA+ inserted at Xho1 site. A library-specific adaptor (GCTGGCTGG) was used to verify the library identity and then trimmed along with vector and low quality sequence. cDNA Library Statistics: Avg. insert length: 1134; Primary Titer: 2E6 pfu total; Amplified Titer: 3.2E10 pfu/ml; Antibiotic: 50 ug/ml Ampicillin. This library was been generated by the Floral Genome Project (FGP; http://fgp.bio.psu.edu) and additional sequencing was done as part of the Ancestral Angiosperm Project (www.AncestralAngiosperm.org). Both projects are funded by NSF's Plant Genome Research Program (DBI-0115684 and DBI-0638595, respectively).

text: premeiotic

text: SOLR (E. coli)

text: Flower buds

text: Nuphar advena

text: Black Moshannon State Park PA

characteristic: strain

text: Flower buds

text: Nuphar advena flower bud library (Wash U)

text: Plasmid


text: Warsaw Agricultural University, Faculty of Horticulture and Landscape Architecture

text: live

text: Generic

text: The cDNA from 1-2 mm female floral buds was digested with MboI restriction enzyme and amplified. The 'subtractome' library was obtained after subtraction using cDNA-DSC method between near isogenic lines: 2gg (gynoecious -ffMMgygy)- used as a driver and B10 (monoecious ffMMGyGy) - used as a tester.

characteristic: description

text: 1-2 mm

text: 1-2 mm flower bud - female

text: Cucumis sativus

text: female (gynoecious)

characteristic: sex

text: female flower bud cucumber cDNA-DSC 2gg/B10


text: live

text: Generic

text: The cDNA from 1-2 mm hermaphrodite floral buds was digested with MboI restriction enzyme and amplified. The 'subtractome' library was obtained after subtraction using cDNA-DSC method between near isogenic lines: HGy3 (hermaphrodite -FFmmGyGy)- used as a tester and Gy3 (gynoecious FFMMGyGy)- used as a driver.

text: 1-2 mm

text: 1-2 mm flower bud - hermaphrodite

characteristic: organ

text: Cucumis sativus

characteristic: organism

text: hermaphrodite

text: hermaphrodite flower bud cucumber cDNA-DSC HGy3/Gy3


text: Advanced Center for Genome Technology, University of Oklahoma

text: live

text: Generic

text: Celuca pugilator late proecdysial regenerating limb cDNA library created by Dr. David Durica at the University of Oklahoma, Department of Zoology, Norman, Oklahoma.

text: adult

characteristic: dev-stage

text: regenerating buds

text: Leptuca pugilator

text: EcoRI

text: XhoI

text: male

text: late proecdysial stage of molt cycle

text: Fiddler crab late proecdysial limb bud cDNA library

characteristic: title

text: phagemid

text: pBlueScriptSK(-) (Stratagene)


text: University of Victoria

text: live

text: Generic

text: Tissue contributors: L. Bernatchez (Laval University)

text: eye, kidney, spleen

text: Coregonus clupeaformis

text: mixed tissue

characteristic: tissue-type

text: ccluevb mixed_tissue Coregonus clupeaformis cDNA

characteristic: vector


characteristic: External Id

text: University of Victoria

text: live

text: Generic

text: Tissue contributors: R. Devlin (DFO, Vancouver, B.C.)

text: brain, kidney, spleen

text: Oncorhynchus nerka

text: mixed tissue

text: onerrge mixed_tissue Oncorhynchus nerka cDNA


text: University of Victoria

text: live

text: Generic

text: brain, spleen, kidney, liver

text: Salvelinus fontinalis

text: mixed tissue

text: sfonevi mixed_tissue Salvelinus fontinalis cDNA


text: University of Victoria

text: live

text: Generic

text: Tissue contributors: W. Davidson (Simon Fraser University)

text: brain, kidney, heart, pyloric caeca

text: Esox lucius

text: mixed tissue

text: elucbkhp mixed_tissue Esox lucius cDNA

text: pDNR-LIB


text: University of Victoria

text: live

text: Generic

text: Tissue contributors: N. Sherwood (University of Victoria)

text: brain, pituitary

text: Thymallus arcticus

text: mixed tissue

text: tarcevg mixed_tissue Thymallus arcticus cDNA


text: Centro de Investigacion Sobre Enfermedades Infeccionsas, Instituto Nacional de Salud Publica

text: live

text: Generic

text: Adult female Anopheles albimanus salivary gland cDNA library

text: Adult (3 days post-emergence)

text: Salivary gland

text: Anopheles albimanus

text: Female

text: white stripe

text: Plasmid

text: pBSKS


text: live

text: Generic

text: Subtractive fatbody cDNA library generated from Plasmodium vivax-infected mosquitoes (15-16 days post infective blood meal) versus non-infected mosquitoes using the PCR-Select cDNA subtraction kit (Clontech).

text: Adult

text: Fatbody and abdominal cuticle

text: Anopheles albimanus

text: Not I

text: Female

text: white stripe

text: Plasmid

text: pBSKS


text: live

text: Generic

text: Subtractive midgut cDNA library generated from Plasmodium vivax-infected mosquitoes (15-16 days post infective blood meal) versus non-infected mosquitoes using the PCR-Select cDNA subtraction kit (Clontech).

text: Adult (3 days post-emergence)

text: Whole mosquito

text: Anopheles albimanus

text: Not I

text: Female

text: white stripe

text: Plasmid

text: pBSKS


text: live

text: Generic

text: Anopheles albimanus females challenged with Serratia marcescens.

text: Adult (3 days post-emergence)

text: Whole mosquito

text: Anopheles albimanus

text: Sfi I

text: Sfi I

text: Female

text: white stripe

text: Plasmid

text: pDNR-LIB


text: University of Arizona

text: live

text: Generic

text: Adult

text: ElectroMAX DH10B - T1 electrocompetent cells

characteristic: lab-host

text: lower reproductive tract (oviduct, uterus, parovaria, spermathecae, seminal receptacle)

text: Drosophila arizonae

text: female

text: TUC 1205

text: Drosophila arizonae female reproductive transcripts

text: plasmid


text: University of Manchester

characteristic: INSDC center name

text: live

text: Generic

text: Adult

text: Spinal cord

text: Platichthys flesus

text: Wild type

text: Flounder CNSS library


text: Bellarmine University

characteristic: INSDC first public

text: live

text: Generic

text: Mature male flowers and pollen were collected from a single weedy population of Ambrosia trifida growing in Louisville, KY, USA. mRNA was isolated using the Invitrogen FastTrack 2.0 Kit. The cDNA Library was generated using the CloneMiner cDNA Library Construction Kit (Invitrogen). Sequences have been trimmed to exclude vector.

text: Mature flowers, pre-dehiscent

text: E. coli

text: Male flower and pollen

text: Ambrosia trifida

text: BsrGI

text: BsrGI

text: Monoecious

text: Reproductive

text: Ragweed male flower and pollen cDNA library

text: Plasmid


text: The Royal Veterinary and Agricultural University

characteristic: INSDC last update

text: live

text: Generic

text: M. Biceps femoris from DIAS (Danish Institute of Agricultural Sciences).

text: M. Biceps femoris

text: Sus scrofa

text: bfe


text: live

characteristic: INSDC status

text: Generic

text: Adrenal gland from KVL (Royal Veterinary and Agricultural University), RNA from BGI (Beijing Genomics Institute).

text: Adrenal gland

text: Sus scrofa

text: cag


text: live

text: Generic

characteristic: NCBI submission model

text: Galactophore from KVL (Royal Veterinary and Agricultural University), RNA from BGI (Beijing Genomics Institute).

text: Galactophore

text: Sus scrofa

text: cga


text: live

text: Generic

characteristic: NCBI submission package

text: Rhinal mucous membrane from DIAS (Danish Institute of Agricultural Sciences), RNA from BGI (Beijing Genomics Institute).

text: Rhinal mucous membrane

text: Sus scrofa

text: cmu


text: live

text: Generic

text: Large intestine, colon ascendens from KVL (Royal Veterinary and Agricultural University), RNA from BGI (Beijing Genomics Institute).

text: Large intestine, colon ascendens

text: Sus scrofa

text: col


text: live

text: Generic

text: Small intestine, duodenum from KVL (Royal Veterinary and Agricultural University)

text: Small intestine, duodenum

text: Sus scrofa

text: duo


text: live

text: Generic

text: Foetus 50 days, cortex cerebri from KVL (Royal Veterinary and Agricultural University)

text: Foetus 50 days

text: Cortex cerebri

text: Sus scrofa

text: ecc


text: live

text: Generic

text: Foetus 50 days, regio umbilicalis from KVL (Royal Veterinary and Agricultural University)

text: Foetus 50 days

text: Regio umbilicalis

text: Sus scrofa

text: eru


text: live

text: Generic

text: Foetus 50 days, M. semitendinosus from KVL (Royal Veterinary and Agricultural University)

text: Foetus 50 days

text: M. semitendinosus

text: Sus scrofa

text: ese


text: live

text: Generic

text: Cortex cerebri (107 days early born) from KVL (Royal Veterinary and Agricultural University)

text: 107 days early born

text: Cortex cerebri

text: Sus scrofa

text: fcc


text: live

text: Generic

text: Frontal cortex from DIAS (Danish Institute of Agricultural Sciences)

text: Frontal cortex

text: Sus scrofa

text: fco


text: live

text: Generic

text: Hippocampus (107 days early born) from KVL (Royal Veterinary and Agricultural University)

text: 107 days early born

text: Hippocampus

text: Sus scrofa

text: fhi


text: live

text: Generic

text: Heart, left ventricle from KVL (Royal Veterinary and Agricultural University)

text: Heart, left ventricle

text: Sus scrofa

text: hlv


text: live

text: Generic

text: Hypothalamus from KVL (Royal Veterinary and Agricultural University)

text: Hypothalamus

text: Sus scrofa

text: hyp


text: live

text: Generic

text: Small Intestine, illeum from KVL (Royal Veterinary and Agricultural University)

text: Small Intestine, illeum

text: Sus scrofa

text: ill


text: live

text: Generic

text: M. Infraspinatus from DIAS (Danish Institute of Agricultural Sciences)

text: M. Infraspinatus

text: Sus scrofa

text: isp


text: The Royal Veterinary and Agricultural University

text: live

text: Generic

text: Joint capsule from KVL (Royal Veterinary and Agricultural University)

text: Joint capsule

text: Sus scrofa

text: jca


text: live

text: Generic

text: Small intestine, jejunum from KVL (Royal Veterinary and Agricultural University)

text: Small intestine, jejunum

text: Sus scrofa

text: jej


text: live

text: Generic

text: M. Longissimus dorsi from DIAS (Danish Institute of Agricultural Sciences)

text: M. Longissimus dorsi

text: Sus scrofa

text: ldo


text: live

text: Generic

text: Large intestine from DIAS (Danish Institute of Agricultural Sciences)

text: Large intestine

text: Sus scrofa

text: lin


text: live

text: Generic

text: Lymphatic nodule tracheobroncalis from KVL (Royal Veterinary and Agricultural University)

text: Lymphatic nodule tracheobroncalis

text: Sus scrofa

text: lnt


text: live

text: Generic

text: Lymphatic gland from DIAS (Danish Institute of Agricultural Sciences)

text: Lymphatic gland

text: Sus scrofa

text: lyg


text: live

text: Generic

text: M. masseter from KVL (Royal Veterinary and Agricultural University)

text: M. masseter

text: Sus scrofa

text: mas


text: live

text: Generic

text: Mediastinum from KVL (Royal Veterinary and Agricultural University)

text: Mediastinum

text: Sus scrofa

text: med


text: live

text: Generic

text: Mammary gland - 7 days after weaning from DIAS (Danish Institute of Agricultural Sciences)

text: 7 days after weaning

text: Mammary gland

text: Sus scrofa

text: mga


text: live

text: Generic

text: Mammary gland - 14 days after birth from DIAS (Danish Institute of Agricultural Sciences)

text: 14 days after birth

text: Mammary gland

text: Sus scrofa

text: mgm


text: live

text: Generic

text: Mammary gland - 7 days pre birth from DIAS (Danish Institute of Agricultural Sciences)

text: 7 days pre birth

text: Mammary gland

text: Sus scrofa

text: mgp


text: live

text: Generic

text: Newborn 115 days, bone marrow (pelvis) from KVL (Royal Veterinary and Agricultural University)

text: Newborn 115 days

text: Bone marrow (pelvis)

text: Sus scrofa

text: nbm


text: live

text: Generic

text: Newborn 115 days, mucosal membranes from KVL (Royal Veterinary and Agricultural University)

text: Newborn 115 days

text: Mucosal membranes

text: Sus scrofa

text: nmm


text: live

text: Generic

text: Newborn 115 days, M. semitendinosus from KVL (Royal Veterinary and Agricultural University)

text: Newborn 115 days

text: M. semitendinosus

text: Sus scrofa

text: nms


text: live

text: Generic

text: Pituitary gland from DIAS (Danish Institute of Agricultural Sciences)

text: Pituitary gland

text: Sus scrofa

text: pgl


text: live

text: Generic

text: Salivary gland from DIAS (Danish Institute of Agricultural Sciences)

text: Salivary gland

text: Sus scrofa

text: sag


text: The Royal Veterinary and Agricultural University

text: live

text: Generic

text: Small intestine from DIAS (Danish Institute of Agricultural Sciences)

text: Small intestine

text: Sus scrofa

text: sin


text: live

text: Generic

text: M. Semimembranosus from DIAS (Danish Institute of Agricultural Sciences)

text: M. Semimembranosus

text: Sus scrofa

text: sme


text: live

text: Generic

text: Spinal cord from DIAS (Danish Institute of Agricultural Sciences)

text: Spinal cord

text: Sus scrofa

text: spc


text: live

text: Generic

text: M. Supraspinatus from DIAS (Danish Institute of Agricultural Sciences)

text: M. Supraspinatus

text: Sus scrofa

text: ssp


text: live

text: Generic

text: M. Semitendinosus from DIAS (Danish Institute of Agricultural Sciences)

text: M. Semitendinosus

text: Sus scrofa

text: ste


text: live

text: Generic

text: Suprarenal gland from DIAS (Danish Institute of Agricultural Sciences)

text: Suprarenal gland

text: Sus scrofa

text: sug


text: live

text: Generic

text: M. Triceps brachii from DIAS (Danish Institute of Agricultural Sciences)

text: M. Triceps brachii

text: Sus scrofa

text: tbr


text: live

text: Generic

text: Thyroid gland from DIAS (Danish Institute of Agricultural Sciences)

text: Thyroid gland

text: Sus scrofa

text: thg


text: live

text: Generic

text: Tip of tongue, mucosal membrane from KVL (Royal Veterinary and Agricultural University)

text: Tip of tongue

text: Sus scrofa

text: mucosal membrane

text: ton


text: live

text: Generic

text: M. Vastus intermedius from DIAS (Danish Institute of Agricultural Sciences).

text: M. Vastus intermedius

text: Sus scrofa

text: vin


text: Ensis: the joint forces of CSIRO and Scion

text: live

text: Generic

text: vertical stem

text: Eucalyptus grandis

text: hermaphrodite

text: Developing xylem

text: nine-year-old eucalypt xylem library


text: Microbial Ecology, Institution of Ecology

text: live

text: Generic

text: Nutrient patch (NH4+)

text: Paxillus involutus

text: SfiA

text: SfiA

characteristic: strain

text: mycelium

text: DW1E (Nitrogen nutrient patch - microcosm)

text: Plasmid


text: University of Victoria

text: live

text: Generic

text: Tissue contributors: N. Sherwood (University of Victoria)

characteristic: description

text: brain, pituitary

text: Sebastes rastrelliger

text: mixed tissue

text: srasevg mixed_tissue Sebastes rastrelliger cDNA


text: The Natural History Museum

text: live

text: Generic

text: An cDNA library, enriched for differentially expressed genes, was sequenced using M13F. Adaptor primer sequences were removed from sequence.

text: Haemopoietic organ

text: Biomphalaria glabrata

text: Hermaphrodite

characteristic: sex

text: NHM 3017

text: BgSSH (R-S) schistosome-exposed NHM 3017 Haemopoietic organ

text: pGEM


text: The Natural History Museum

text: live

text: Generic

text: Haemopoietic organ

characteristic: organ

text: Biomphalaria glabrata

text: Hermaphrodite

text: NHM 1742

text: BgSSH (S-R) schistosome-exposed NHM 1742 Haemopoietic organ

text: pGEM


text: The Natural History Museum

text: live

text: Generic

text: Haemopoietic organ

text: Biomphalaria glabrata

characteristic: organism

text: Hermaphrodite

text: NHM 3017

text: BgSSH (Un-Ex) unexposed NHM 3017 Haemopoietic organ

text: pGEM


text: The Natural History Museum

text: live

text: Generic

text: Haemopoietic organ

text: Biomphalaria glabrata

text: Hermaphrodite

text: NHM 3017

text: BgSSH (R-S) schistosome-exposed NHM 3017 Haemopoietic organ 2

text: pGEM


text: The Natural History Museum

text: live

text: Generic

text: Haemopoietic organ

text: Biomphalaria glabrata

text: Hermaphrodite

text: NHM 1742

text: BgSSH (S-R) schistosome-exposed NHM 1742 Haemopoietic organ 2

text: pGEM


text: The Natural History Museum

text: live

text: Generic

text: Haemopoietic organ

text: Biomphalaria glabrata

text: Hermaphrodite

text: NHM 3017

text: BgSSH (Un-Ex) unexposed NHM 3017 Haemopoietic organ 2

text: pGEM


text: The Natural History Museum

text: live

text: Generic

text: Haemopoietic organ

text: Biomphalaria glabrata

text: Hermaphrodite

text: NHM 3017

text: BgSSH (Ex-Un) schistosome-exposed NHM 3017 Haemopoietic organ 2

text: pGEM


text: National Institute of Agricultural Biotechnology (NIAB http://www.niab.go.kr/homepage/english/), Rural Development Administration (RDA http://www3.rda.go.kr/eng/)

text: live

text: Generic

text: Chiifu

characteristic: cultivar

text: A 5-weeks-old B. rapa plant grown in green house was transferred at 4 oC growth chamber and then subjected to cold stress for 12 hours under fluorescent light and whole plants was used for the total RNA extraction. The cDNA library was constructed using lambda ZAP-cDNA synthesis kit (Stratagene, USA) according to the manufacturer's instructions and randomly selected cDNA clones were used for the 5-end sequence analysis.

text: 5-weeks-old plant

characteristic: dev-stage

text: whole plant

text: Brassica rapa subsp. pekinensis

text: Eco RI

text: Xho I

text: light-chilled whole plant cDNA library KBCD

text: pBluescriptII SK(-)


text: live

text: Generic

text: Chiifu

text: Floral buds in size of 3 - 6 mm were used for the total RNA extraction. The cDNA library was constructed using lambda ZAP-cDNA synthesis kit (Stratagene, USA) according to the manufacturer's instructions and randomly selected cDNA clones were used for the 5-end sequence analysis.

text: floral buds in 3 - 6 mm size

text: floral buds

text: Brassica rapa subsp. pekinensis

text: Eco RI

text: Xho I

text: floral bud cDNA library KBFL

characteristic: title

text: pBluescriptII SK(-)


text: live

text: Generic

text: Chiifu

text: Floral buds in size of 1 - 3 mm were used for the total RNA extraction. The cDNA library was constructed using lambda ZAP-cDNA synthesis kit (Stratagene, USA) according to the manufacturer's instructions and randomly selected cDNA clones were used for the 5-end sequence analysis.

text: floral buds in 1 - 3 mm size

text: floral buds

text: Brassica rapa subsp. pekinensis

text: Eco RI

text: Xho I

text: floral buds cDNA library KBFS

text: pBluescriptII SK(-)

characteristic: vector


characteristic: External Id

text: live

text: Generic

text: Chiifu

text: The root parts of 3-weeks-old B. rapa plants grown in horticultural soil were soaked in 250 mM NaCl solution. The plants were collected after 1hr, 6hr, 12hr, and 24hr treatment and these were used for the RNA extraction. The cDNA library was constructed using lambda ZAP-cDNA synthesis kit (Stratagene, USA) according to the manufacturer's instructions and randomly selected cDNA clones were used for the 5-end sequence analysis.

text: 3-weeks-old plants treated with 250 mM NaCl

text: whole plant

text: Brassica rapa subsp. pekinensis

text: Eco RI

text: Xho I

text: salt-treated whole plant cDNA library KBLS

text: pBluescriptII SK(-)


text: live

text: Generic

text: Chiifu

text: The etiolated mature inner leaves of fully grown B. rapa plant were used for the RNA extraction. The cDNA library was constructed using lambda ZAP-cDNA synthesis kit (Stratagene, USA) according to the manufacturer's instructions and randomly selected cDNA clones were used for the 5-end sequence analysis.

text: mature leaf

text: etiolated mature leaf

text: Brassica rapa subsp. pekinensis

text: Eco RI

text: Xho I

text: etiolated mature leaf cDNA library KBLW

text: pBluescriptII SK(-)


text: live

text: Generic

text: Chiifu

text: RNA was extracted from the primary inflorescence during the flowering stage. The cDNA library was constructed using lambda ZAP-cDNA synthesis kit (Stratagene, USA) under the manufacturer's instructions and randomly selected cDNA clones were used for the 5-end sequence analysis.

text: flowering

text: primary inflorescence

text: Brassica rapa subsp. pekinensis

text: Eco RI

text: Xho I

text: primary inflorescence cDNA library KBST

text: pBluescriptII SK(-)


text: live

text: Generic

text: Chiifu

text: RNA was extracted from the mixtures including diverse sizes of floral buds and open flowers. The SfiI digested double-stranded cDNAs were inserted into DraI digested pCNS-D2 vector and cDNA library was constructed using full-length enrichment methods (www.corebio.com).

text: mixed developmental stages of floral organs including diverse sizes of floral buds and open flowers

text: floral organ

text: Brassica rapa subsp. pekinensis

text: Dra 1A

text: Dra 1B

text: flower cDNA library KFFB


text: live

text: Generic

text: Chiifu

text: Mixed tissues of dried mature seeds and 2-days-old seedlings of B. rapa plant were used for the RNA extraction. The SfiI digested double-stranded cDNAs were inserted into DraI site of pCNS-D2 vector and cDNA library was constructed using full-length enrichment methods (www.corebio.com).

text: mixture of mature dried seeds and 2-days-old seedling

text: mixture of seeds and 2-days-old seedling

text: Brassica rapa subsp. pekinensis

text: Dra 1A

text: Dra 1B

text: seed and seedling cDNA library KFSD


text: live

text: Generic

text: Chiifu

text: The whole parts of 3-weeks-old B. rapa plant were used for the RNA extraction. The SfiI digested double-stranded cDNAs were inserted into DraI site of pCNS-D2 vector and cDNA library was constructed using full-length enrichment methods (www.corebio.com).

text: whole plant

text: Brassica rapa subsp. pekinensis

text: Dra 1A

text: Dra 1B

text: whole plant cDNA library KFYP


text: live

text: Generic

text: Chiifu

text: Poly (A+)-mRNA was isolated from total RNA using paramagnetic oligo (dT) beads (PolyAtract mRNA isolationII, Promega) according to the manufacturer's procedures. The cDNAs were cloned into lambda ZAPII (Stratagene) and random clones selected for end sequence verification.

text: Mixed stage of seed development (ovule and silique)

text: Ovule and silique

text: Brassica rapa subsp. pekinensis

text: EcoRI

text: XhoI

text: ovule and silique cDNA library KHOS

text: pBluescriptII SK(-)


text: National Institute of Agricultural Biotechnology (NIAB http://www.niab.go.kr/homepage/english/), Rural Development Administration (RDA http://www3.rda.go.kr/eng/)

text: live

text: Generic

text: Chiifu

text: The primary leaf of 1-weeks-old B. rapa grown in MS salt media were used for the RNA extraction. The cDNA library was constructed using CreatorTM SMARTTM cDNA Construction kit (Clonetech, USA) according to the manufacturer's instructions and randomly selected cDNA clones were used for the 5-end sequence analysis.

text: primary leaf of 1-week-old B. rapa

text: primary leaf

text: Brassica rapa subsp. pekinensis

text: Sfi IA

text: Sfi IA

text: primary leaf cDNA library KLPS

text: pDNR-LIB


text: live

text: Generic

text: Chiifu

text: The mixed tissues of floral bud and open flower of B. rapa plant were used for the RNA extraction. The SfiI digested double-stranded cDNAs were inserted into DraI site of pCNS-D2 vector and cDNA library was constructed using full-length enrichment methods and then normalized following the protocols (www.corebio.com).

text: mixed stages of floral buds and open flower

text: floral organ

text: Brassica rapa subsp. pekinensis

text: Dra 1A

text: Dra 1B

text: normalized flower cDNA library NRFB


text: National University of Singapore

text: live

text: Generic

text: Total RNA and mRNA were isolated and purified following the protocols of the RNeasy Maxi Kit and the Oligotex mRNA Mini Kit, respectively (QIAGEN). The cDNA library was constructed following the protocols of the ZAP-cDNA Synthesis Kit (Stratagene). Plasmids were isolated and inserts end-sequenced by T3 promoter primer. Traces were processed for submission to dbEST.

text: XLI-blue

characteristic: lab-host

text: Whole organism

text: Dermatophagoides pteronyssinus

text: EcoR I

text: Xho I

text: Male and female

text: Dermatophagoides pteronyssinus cDNA library

text: Plasmid

text: pBluescriptSK(-)(Stratagene)


text: The University of Melbourne

characteristic: INSDC center name

text: live

text: Generic

text: Total RNA isolated from tammar wallaby mammary gland during lactation (days 55, 87, 130, 180, 220, 260) and involution (5 days after removal of pouch young at 45d lactation) were pooled and used for normalised library construction. Subsequent cDNA molecules were directionally cloned into pMCVSport6-ccdB vector using NotI (3') and EcoRV (5') sites. Quality control of the resulting library revealed a titre of 1.2 x 10e5 cfu/ml with a mean insert size of 1.2kb. Southern hybridisation for abundance of alpha-casein cDNA molecules revealed a reduction of 37 fold (7.8% in the non-normalised library, 0.2% in the normalised library).

text: mix of mammary tissues from day 55,87,130,180,220,260 of lactation and 5 days involution after 45 days of lactation

text: mammary gland

text: Notamacropus eugenii

text: NotI

text: EcoRV

text: female

text: mammary gland

characteristic: tissue-type

text: normalized library from a mix of tammar wallaby mammary tissues

text: Plasmid


text: The University of Melbourne

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: The cDNA sequences were produced from lactation stage specific cDNA cloning of mRNA obtained from day 23 pregnant, day 130, day 260 of lactation or 4 days unsucked gland. First strand cDNA was prepared from total RNA (1ug) or mRNA (500ng) using the SMART II oligonucleotide and MMLV reverse transcriptase as recommended by the manufacturer (Promega) except for library 15 which used a thermostable reverse transcriptase (Thermoscript, Invitrogen). PCR was used to make second strand cDNA according the the SMART system instructions (Promega). The first strand cDNA was then ligated with pGEMTeasy (Promega) using the recommended ligation reaction conditions and reagents. In some cases, the second strand cDNA was treated further prior to ligation when size selection of cDNA molecules was achieved by two methods. For libraries 5-10, the cDNA was fractionated by agarose gel electrophoresis and fragments of different sizes were purified using silicon beads (Qiaex beads, Qiagen). For libraries 16-19, small cDNA fragments were removed by sephadex column chromatography using a CHROMA SPIN-1000 column (Promega). Electroporation was used to transform E.coli DH5a with ligated material and recombinant bacteria were selected for ampicillin resistance.

text: day 130 of lactation

text: mammary gland

text: Notamacropus eugenii

text: female

text: mammary gland

text: library_1: tammar wallaby mammary tissues from day 130 lactation

text: Plasmid

text: pGEMTeasy


text: The University of Melbourne

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: day 130 of lactation

text: mammary gland

text: Notamacropus eugenii

text: female

text: mammary gland

text: library_2: tammar wallaby mammary tissues from day 130 lactation

text: Plasmid

text: pGEMTeasy


text: The University of Melbourne

text: live

text: Generic

characteristic: NCBI submission package

text: day 130 of lactation

text: mammary gland

text: Notamacropus eugenii

text: female

text: mammary gland

text: library_3: tammar wallaby mammary tissues from day 130 lactation

text: Plasmid

text: pGEMTeasy


text: The University of Melbourne

text: live

text: Generic

characteristic: description

text: day 130 of lactation

text: mammary gland

text: Notamacropus eugenii

text: female

text: mammary gland

text: library_4: tammar wallaby mammary tissues from day 130 lactation

text: Plasmid

text: pGEMTeasy


text: The University of Melbourne

text: live

text: Generic

text: Subtracted for alpha-lactalbumin, beta-lactoglobulin, alpha-casein, beta-casein and kappa-casein. The cDNA sequences were produced from lactation stage specific cDNA cloning of mRNA obtained from day 23 pregnant, day 130, day 260 of lactation or 4 days unsucked gland. First strand cDNA was prepared from total RNA (1ug) or mRNA (500ng) using the SMART II oligonucleotide and MMLV reverse transcriptase as recommended by the manufacturer (Promega) except for library 15 which used a thermostable reverse transcriptase (Thermoscript, Invitrogen). PCR was used to make second strand cDNA according the the SMART system instructions (Promega). The first strand cDNA was then ligated with pGEMTeasy (Promega) using the recommended ligation reaction conditions and reagents. In some cases, the second strand cDNA was treated further prior to ligation when size selection of cDNA molecules was achieved by two methods. For libraries 5-10, the cDNA was fractionated by agarose gel electrophoresis and fragments of different sizes were purified using silicon beads (Qiaex beads, Qiagen). For libraries 16-19, small cDNA fragments were removed by sephadex column chromatography using a CHROMA SPIN-1000 column (Promega). Electroporation was used to transform E.coli DH5a with ligated material and recombinant bacteria were selected for ampicillin resistance. Subtracted libraries were also produced; for two libraries (11 and 12), cDNA for alpha-lactalbumin, beta-lactoglobulin, alpha-casein, beta-casein and kappa-casein was removed from the cDNA pool following the manufacturers instructions for the SMART subtraction library (Promega) except that the subtracted material used was double stranded cDNA corresponding to the five genes listed above

text: day 130 of lactation

text: mammary gland

text: Notamacropus eugenii

text: female

characteristic: sex

text: mammary gland

text: library_11: tammar wallaby mammary tissues from day 130 lactation (Subtracted)

text: Plasmid

text: pGEMTeasy


text: The University of Melbourne

text: live

text: Generic

text: day 130 of lactation

text: mammary gland

characteristic: organ

text: Notamacropus eugenii

text: female

text: mammary gland

text: library_12: tammar wallaby mammary tissues from day 130 lactation (Subtracted)

text: Plasmid

text: pGEMTeasy


text: The University of Melbourne

text: live

text: Generic

text: day 130 of lactation

text: mammary gland

text: Notamacropus eugenii

characteristic: organism

text: female

text: mammary gland

text: library_13: tammar wallaby mammary tissues from day 130 lactation

text: Plasmid

text: pGEMTeasy


text: The University of Melbourne

text: live

text: Generic

text: day 260 of lactation

text: mammary gland

text: Notamacropus eugenii

text: female

text: mammary gland

text: library_14: tammar wallaby mammary tissues from day 260 lactation

text: Plasmid

text: pGEMTeasy


text: The University of Melbourne

text: live

text: Generic

text: day 23 pregnant

text: mammary gland

text: Notamacropus eugenii

text: female

text: mammary gland

text: library_15: tammar wallaby mammary tissues from day 23 pregnancy

text: Plasmid

text: pGEMTeasy


text: The University of Melbourne

text: live

text: Generic

text: day 23 pregnant

text: mammary gland

text: Notamacropus eugenii

text: female

text: mammary gland

text: library_16: tammar wallaby mammary tissues from day 23 pregnancy

text: Plasmid

text: pGEMTeasy


text: The University of Melbourne

text: live

text: Generic

text: unsucked gland at 4 days of lactation

text: mammary gland

text: Notamacropus eugenii

text: female

text: mammary gland

text: library_18: tammar wallaby mammary tissues from unsucked gland at 4 days of lactation

text: Plasmid

text: pGEMTeasy


text: The University of Melbourne

text: live

text: Generic

text: unsucked gland at 4 days of lactation

text: mammary gland

text: Notamacropus eugenii

text: female

text: mammary gland

text: library_19: tammar wallaby mammary tissues from unsucked gland at 4 days of lactation

text: Plasmid

text: pGEMTeasy


text: The University of Melbourne

text: live

text: Generic

text: day 23 pregnant

text: mammary gland

text: Notamacropus eugenii

text: female

text: mammary gland

text: library_17: tammar wallaby mammary tissues from day 23 pregnancy

text: Plasmid

text: pGEMTeasy


text: University of Hawaii

text: live

text: Generic

text: SunUp

characteristic: cultivar

text: Including leaves from young seedlings and mature trees, roots from young seedlings, stem from young seedlings, flowers from female, male, and hermaphrodite plants before meiosis and after meiosis stage, and fruits and seeds of 10 different stages from 7 days after pollination to 100% ripe fruits. The cDNA library was constructed and normalized to reduce redundancy by subtraction.

text: mixed

text: E. coli

text: leaf, root, stem, flower, fruit, and seed

text: Carica papaya

text: Sfi I

text: Sfi I

text: female, male, and hermaphrodite

text: Plasmid

text: pDNR-LIB


text: Kasetsart University

text: live

text: Generic

text: androgenin gland

text: Macrobrachium rosenbergii

text: Macrobrachium rosenbergii androgenin gland


text: Medical School of Ribeirao Preto, University of Sao Paulo

text: live

text: Generic

text: The SSH cDNA library was constructed using the SMART PCR cDNA synthesis and the PCR-Select cDNA Subtraction Kit (Clontech Laboratories, Inc., Palo Alto, CA) according to the manufacturer's protocol. Subtraction was conducted by using WARs hippocampi as tester and Wistar hippocampi as driver.

text: hippocampus

text: Rattus norvegicus

text: ECO RV

text: male

text: Wistar Audiogenic Rat

characteristic: strain

text: Wistar Audiogenic Rat SSH cDNA library

text: pGEM-T


text: Universite Laval

text: live

text: Generic

text: Tender root tips free of michorizee

text: Activelyl elongating roots

characteristic: dev-stage

text: E. coli DH10B cells

text: Roots from 60 cm tall seedlings

text: Picea glauca

text: Eco-RI

text: Xho-I

text: Hermaphrodite

text: Tender roots tips free of michorizee

text: GQ013 - Elongating ROOTS tips - sapplings

characteristic: title

text: Plasmid

text: pBluescript II SK (+) XR Plasmid


characteristic: External Id

text: Universite Laval

text: live

text: Generic

text: Library synthesis was performed by Evrogen using methodology described in Zhu et al. (2001) Biotechniques 30: 892-897. Starting material is 0,3 ug of total RNA

text: Pool of trees sampled at three different times (22H00, 1H00, 4H00) during the night

text: Stem from 12 year old trees

text: Picea glauca

text: Hermaphrodite (Monoecious)

text: 3 unknown genotypes, 12 year old plantation, St-Leonard-de-Portneuf, Quebec

text: Non-lignified differentiating secondary xylem from normal vertical trees

text: GQ029 - Xylem scrapings - AT NITE

text: Plasmid

characteristic: vector


text: Universite Laval

characteristic: INSDC center name

text: live

text: Generic

text: Wood property progeny test (Reserve de Mastigouche, Quebec). 1 tree from each of 40 families. Approx 27 years of age. Trees were pooled at time of collection. Collected on July 20 pm -21 am. Library synthesis was performed by Evrogen using methodology described in Zhulidov et al. (2004) Nucleic Acids Res. 32, e37. Starting material is 0,3 ug of total RNA.

text: Collected on July 20 pm -21 am

characteristic: lab-host

text: Stem from 27 year old trees

text: Picea glauca

text: Hermaphrodite (Monoecious)

text: Non-lignified differentiating secondary xylem from normal vertical trees, 2 m above ground level, one log approx.50 cm in length. All trees were pooled

text: GQ031 - Xylem Scrapings (Normalized library)

text: Plasmid


text: Universite Laval

characteristic: INSDC first public

text: live

text: Generic

text: Library synthesis was performed by Evrogen using methodology described in Zhulidov et al. (2004) Nucleic Acids Res. 32, e37. Starting material is 0,3 ug of total RNA.

text: Tissues collected weekly, from 1st week in July to 30 October, 2005. 2 seedlings per collection date

text: Terminal leader and branches with needles (pooled from whole plant)

text: Picea glauca

text: Hermaphrodite (Monoecious)

text: QTL population large seedlings, Family 28-56. Rooted cuttings, rooted in 2002. Destructive sampling. Size of plants 30+ cm

text: Shoot tip collected from terminal leader and from branches with needles (pooled from whole plant)

characteristic: tissue-type

text: GQ032 - Shoot tip (Normalized library)

text: Plasmid


text: Universite Laval

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Strain: Wood property progeny test (Reserve de Mastigouche, Quebec). 1 tree from each of 40 families. Approx 27 years of age. Trees were pooled at time of collection. Collected on July 20 pm -21 am. Library synthesis was performed by Evrogen using methodology described in Zhulidov et al. (2004) Nucleic Acids Res. 32, e37. Starting material is 0,3 ug of total RNA.

text: Collected on July 20 pm -21 am

text: Terminal leader

text: Picea glauca

text: Hermaphrodite (Monoecious)

text: Terminal leader, without neeldes, terminal bud and sub-terminal buds. Stem piece 2-3 cm in length (includes bark)

text: GQ033 - Terminal leader (Normalized library)

text: Plasmid


text: Universite Laval

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Trees in third growth cycle, sampled during active growth, at mid season (2005 07 07)

text: Needles from main stem and branches of current season and previous seasons' growth

text: Picea glauca

text: Hermaphrodite (Monoecious)

text: QTL population 2856, tree 6

text: Needles

text: GQ034 - Needles - Mid-season

text: Plasmid


text: Universite Laval

text: live

text: Generic

text: Library synthesis was performed by Evrogen using methodology described in Zhu et al. (2001) Biotechniques 30: 892-897. Starting material is 0,3 ug of total RNA

characteristic: description

text: Trees sampled after completion of third growth cycle (2005 10 18)

text: Picea glauca

text: Hermaphrodite (Monoecious)

characteristic: sex

text: QTL population 2856, multiple individuals, individuals not recorded

text: Needles

text: GQ035 - Needles - End of season

text: Plasmid


text: Universite Laval

text: live

text: Generic

text: Tissue: Actively growing shoot tips, approximately 3 mm in length with developing needles removed. Sample comprises shoot apex, leaf primordia and very small developing leaves, as well as subtending developing primary stem tissues. Library synthesis was performed by Evrogen using methodology described in Zhu et al. (2001) Biotechniques 30: 892-897. Starting material is 0,3 ug of total RNA

text: Actively growing trees sampled 9 weeks after commencement of second growth cycle, no sign of terminal bud formation, corresponds to Day 0 of budset roadmap

text: Terminal shoot tip undergoing active growth

characteristic: organ

text: Picea glauca

characteristic: organism

text: Hermaphrodite (Monoecious)

text: Three individuals from an association population, representing families 646, 800, and 806

text: GQ036 - Shoot tip - Active growth

text: Plasmid


text: Universite Laval

text: live

text: Generic

text: Tissue: Dormant shoot tips dissected from buds. Bud scales and developing needles were removed, leaving a piece of tissue approximately 3 mm in length. Sample comprises shoot apex, leaf primordia as well as subtending primary stem tissues. Endodormant trees: after completion of second growth cycle, trees were transferred progressively from 20 deg C days/20 deg C nights to 20 deg C days/10 deg C nights for 3 weeks, 4 deg C days/4 deg C nights for 2 weeks, and then 2 deg C days/2 deg C nights until sampling. Trees were at 2 deg C for 5.5 weeks prior to sampling. Library synthesis was performed by Evrogen using methodology described in Zhu et al. (2001) Biotechniques 30: 892-897. Starting material is 0,3 ug of total RNA

text: Endodormant trees

text: Terminal bud

text: Picea glauca

text: Hermaphrodite (Monoecious)

text: Five individuals from an association population, representing families 370, 372, 663, 685, and 986

characteristic: strain

text: Dormant shoot tips dissected from buds

text: GQ037 - Shoot tip - Dormant

text: Plasmid


text: Universite Laval

text: live

text: Generic

characteristic: dev-stage

text: Secondary stem representing current year's growth, sampled from main axis of seedlings

text: Picea glauca

text: Hermaphrodite (Monoecious)

text: Five individuals from an association population, representing families 367, 434, 800, 806 and 807

text: Secondary stem comprising secondary xylem, secondary phloem, cambial region, and bark

text: GQ038 - Stem - Active growth

text: Plasmid


text: Universite Laval

text: live

text: Generic

text: Stage: Dormant trees that have completed second growth cycle. After 9-10 weeks of active growth under 16 h, 20 deg C days/8 h, 20 deg C nights, trees were transferred to 8 h, 20 deg C days/16 h, 20 deg C nights for 10 weeks. Trees were sampled at the end of 10 weeks in short day conditions. Library synthesis was performed by Evrogen using methodology described in Zhu et al. (2001) Biotechniques 30: 892-897. Starting material is 0,3 ug of total RNA

text: Picea glauca

text: Hermaphrodite (Monoecious)

text: Five individuals from an association population, representing families 398, 408, 762, 883, and 1026

text: GQ039 - Stem - Dormant

characteristic: title

text: Plasmid


characteristic: External Id

text: Universite Laval

text: live

text: Generic

text: Tissue: Actively growing shoot tips, approximately 3 mm in length with developing needles removed. Sample comprises shoot apex, leaf primordia and very small developing leaves, as well as subtending developing primary stem tissues. Library synthesis was performed by Evrogen using methodology described in Zhulidov et al. (2004) Nucleic Acids Res. 32, e37. Starting material is 0,3 ug of total RNA.

text: Picea glauca

text: Hermaphrodite (Monoecious)

text: GQ040 - Shoot tip - Active growth (Normalized)

text: Plasmid


text: Universite Laval

text: live

text: Generic

text: Tissue: Dormant shoot tips dissected from buds. Bud scales and developing needles were removed, leaving a piece of tissue approximately 3 mm in length. Sample comprises shoot apex, leaf primordia as well as subtending primary stem tissues. Endodormant trees: after completion of second growth cycle, trees were transferred progressively from 20 deg C days/20 deg C nights to 20 deg C days/10 deg C nights for 3 weeks, 4 deg C days/4 deg C nights for 2 weeks, and then 2 deg C days/ deg C nights until sampling. Trees were at 2 deg C for 5.5 weeks prior to sampling. Library synthesis was performed by Evrogen using methodology described in Zhulidov et al. (2004) Nucleic Acids Res. 32, e37. Starting material is 0,3 ug of total RNA.

text: Endodormant trees

text: Terminal bud

text: Picea glauca

text: Hermaphrodite (Monoecious)

text: Dormant shoot tips dissected from buds

text: GQ041 - Shoot tip - Dormant (Normalized)

text: Plasmid

characteristic: vector


text: ARO, The Volcani Center

characteristic: INSDC center name

text: live

text: Generic

text: Orah

characteristic: cultivar

text: Citrus temple hort. ex Tanaka (Temple tangor) X Citrus reticulata Blanco cultigroup Tangerine cv. 'Dancy Tangerine'. Poly-A mRNA was isolated from total RNA, and cDNA synthesis and library construction was performed using SMART cDNA Library Construction Kit (Clontech laboratories, Inc, Catalog # K1051-1) according to the manufacturer's instructions (Protocol # PT3000-1, Version # PR15738).

text: E. Coli BM25.8

text: Non treated buds during the flowering induction time. The bud popultion has 60% cahnce to flower

text: Citrus reticulata x Citrus temple

text: SfiIA

text: SfiIB

text: Phagemid


text: ARO, The Volcani Center

characteristic: INSDC first public

text: live

text: Generic

text: Orah

text: E. Coli BM25.8

characteristic: lab-host

text: GA-treated buds during flowring induction time. The bud population had 19% chance to flower

text: Citrus reticulata x Citrus temple

text: SfiIA

text: SfiIB

text: Phagemid


text: Dalian Fisheries University

characteristic: INSDC last update

text: live

text: Generic

text: adult

text: regenerating intestine

text: Apostichopus japonicus

text: undifferentiation and dedifferentiation tissue

characteristic: tissue-type

text: Sea cucumber 6/15 days regenerating intestine


text: EBC, Uppsala University

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: hematopoietic organ

text: Pacifastacus leniusculus

text: male

text: Hematopoietic tissue

text: Plhpt, Pacifastacus leniusculus Lambda Zap Express Library HPT cDNA


text: Instituto de Quimica, Universidade de Sao Paulo

text: live

text: Generic

characteristic: NCBI submission package

text: Vomeronasal

text: Mus musculus

text: Vomeronasal epithelium - RLM-RACE 5' ESTs


text: United States Department of Agriculture, Agriculture Research Service, Animal and Natural Resources Institute

text: live

text: Generic

text: The subtracted cDNA library was constructed using Clontech's PCR-Select cDNA subtraction kit, with the SST (tester) and the Vaginal Epithelium (driver) to identify genes unique to the SST.

characteristic: description

text: Mid-Production

text: Vagina and Uterovaginal Junction

text: Meleagris gallopavo

text: Female

characteristic: sex

text: Grade Makers from Hybrid

text: Sperm Storage Tubules (SST) and Vaginal Epithelium

text: Turkey Hen (38 wk old) SST subtracted cDNA library


text: University of Liverpool

text: live

text: Generic

text: cDNA library prepared by Montse

text: Adult

text: Head and Kidney

characteristic: organ

text: Cyprinus carpio

characteristic: organism

text: Montse common carp adult head and kidney

text: Plasmid


text: University of Liverpool

text: live

text: Generic

text: cDNA library prepared by Daryl

text: Adult

characteristic: dev-stage

text: Head & Kidney

text: Cyprinus carpio

text: WeyHK

text: Plasmid


text: USDA-ARS, NPA

text: live

text: Generic

text: (FC504CMS X FC502/2) X SP6322-0, MM)

text: cDNAs were derived from reverse transcription of mRNA after SSH of resistant sugar beet leaf tissue challenged with Cecrospora beticola Sacc.

text: adult

text: 8-week leaf

text: Beta vulgaris

text: whole tissue

text: Sugar beet suppression subtractive hybridisation (SSH) cDNA

characteristic: title


characteristic: External Id

text: National Fisheries Research & Development Institute

text: live

text: Generic

text: head kidney

text: Sebastes schlegelii

text: Sebastes schlegeli head kidney cDNA library


text: Instituto Butantan

text: live

text: Generic

text: es (e-value < e-05).

text: venom glands

text: Loxosceles laeta

text: EcoR I

text: Not I

text: female

text: venom glands

text: Spider Loxosceles laeta cDNA library

characteristic: vector


text: Institute of Parasitology, Biology Centre, ASCR

characteristic: INSDC center name

text: live

text: Generic

text: Secretory

characteristic: cell type

text: PCR-based cDNA library made with SMART cDNA library construction kit from Clontech

text: Adult

text: Salivary gland

text: Ixodes ricinus

text: Female

text: Salivary gland

text: 24 hours after attachment adult female Ixodes ricinus salivary gland library IR24H

text: Phage

text: Lambda TriplEx2


characteristic: INSDC first public

text: live

text: Generic

text: Secretory

text: Adult

text: Salivary gland

text: Ixodes ricinus

text: Female

text: Salivary gland

text: Four days after attachment adult female Ixodes ricinus salivary gland library IR4D

text: Phage

text: Lambda TriplEx2


text: live

text: Generic

text: Secretory

text: Adult

text: Salivary gland

text: Ixodes ricinus

text: Female

text: Salivary gland

text: Seven days after attachment adult female Ixodes ricinus salivary gland library IR7D

text: Phage

text: Lambda TriplEx2


text: live

text: Generic

text: Secretory

text: Adult

text: Salivary gland

text: Ixodes ricinus

text: Female

text: Salivary gland

text: Unfed adult female Ixodes ricinus salivary gland library IRUF

text: Phage

text: Lambda TriplEx2


text: live

text: Generic

text: digestive tract

text: Halocynthia roretzi

text: Halocynthia roretzi digestive tract cDNA library


text: Rajiv Gandhi Centre for Biotechnology

text: live

text: Generic

text: Piper nigrum

text: Monecious

text: Leaf

text: Piper nigrum, leaf-specific subtracted cDNA library clontech (K 1804-1) (RsaI)

text: pGEMT-Easy


text: Colorado State University

text: live

text: Generic

text: Library made from RNA collected from midguts dissected from 50 4-day old adult female Aedes albopictus, 1 day post blood feed.

text: Adult

text: Midgut

text: Aedes albopictus

text: Female

text: Lake Charles

characteristic: strain

text: Aedes albopictus Female midgut


text: Colorado State University

characteristic: INSDC last update

text: live

text: Generic

text: Library made from RNA collected from heads removed from 50 3-day old adult male Aedes albopictus.

text: Adult

text: Head

text: Aedes albopictus

text: Male

text: Lake Charles

text: Aedes albopictus 3 day old male head


text: Colorado State University

text: live

text: Generic

text: Library made from RNA collected from 50 fourth instar larval Aedes albopictus.

text: larva

text: whole body

text: Aedes albopictus

text: Lake Charles

text: Aedes albopictus Fourth instar larvae


text: Colorado State University

text: live

text: Generic

text: Library made from RNA collected from 50 female Aedes albopictus adults within 3 hours post eclosion.

text: adult

text: midgut

text: Aedes albopictus

text: Lake Charles

text: Aedes albopictus Teneral midgut


text: Colorado State University

text: live

text: Generic

text: Library made from RNA collected from ovaries dissected from 50 3-day old adult female Aedes albopictus, not blood fed.

text: adult

text: ovary

text: Aedes albopictus

text: female

text: Lake Charles

text: Aedes albopictus Unfed Ovary


text: Colorado State University

text: live

text: Generic

text: Library made from RNA collected from ovaries dissected from 50 4-day old adult female Aedes albopictus, 1 day post blood feed.

text: adult

text: ovary

text: Aedes albopictus

text: female

text: Lake Charles

text: Aedes albopictus Ovary 1 day post bloodfeed


text: Colorado State University

text: live

text: Generic

text: Library made from RNA collected from ovaries dissected from 50 4-day old adult female Aedes albopictus, 2 day post blood feed.

text: adult

text: ovary

text: Aedes albopictus

text: female

text: Lake Charles

text: Aedes albopictus Ovary 2 day post bloodfeed


text: Research Institute of Pomology and Floriculture

text: live

text: Generic

text: Cacanska Najbolja

characteristic: cultivar

text: cDNA-AFLP was performed to identyfy genes differentially expressed in plum upon Plum pox virus (PPV) infection

text: Prunus domestica

text: leaf

characteristic: tissue-type

text: TDFs from plum cv. Cacanska Najbolja

text: pGEM-T


text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Cacanska Rodna

text: Prunus domestica

text: leaf

text: TDFs from plum cv. Cacanska Rodna

text: pGEM-T


text: Nanjing Forestry University

text: live

text: Generic

characteristic: NCBI submission package

text: Lycoris longituba

text: flower petal

text: Flower petal cDNA library from Lycoris longituba

text: pBluscript


text: The J. Craig Venter Institute

text: live

text: Generic

text: Tissue: low-phosphate treated root, Glomus versiforme infected root, uninoculated root, Sinorhizobium meliloti T202 inoculated root and nodules (3-11 DAI), Sinorhizobium meliloti 102F51 inoculated root and nodules (3-21 DAI), Sinorhizobium meliloti 1021 inoculated root and nodules (28 DAI). Total RNA isolated using TRIzol reagent. No DNaseI digestion was performed. Total RNAs were pooled together. Double strand cDNA were synthesized from pooled RNA using SMART technology (http://www.evrogen.com/t2.shtml). The prepared cDNA was normalized by cDNA denaturation/reassociation, treatment by duplex-specific nuclease (DSN) and amplification of normalized fraction by PCR. The normalized cDNA was then digested with SfiI, size-fractioned, directionally ligated into pDNR-LIB (Clontech) and electroprated into GC10 competent cells (Gene Choice). Clones were sequenced from the 5' end only. RNA provided by Maria Harrison, Sue Miller, Carrol Vance and Vagner Benedito.

characteristic: description

text: Medicago truncatula

text: SfiIA

text: SfiIB

text: mixed root and nodules

text: pDNR-LIB (Clontech)


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: obtained from laboratory culture, University of California, San Diego. The library was made from oligo(dT)-primed cDNA and cloned into the EcoRI (5') and NotI (3') sites of the pT7T3 vector. Library constructed in the laboratory of D. Weisblat at University of California, Berkeley, and sequencing performed at DOE Joint Genome Institute (Walnut Creek, CA).

text: several, midembryogenesis

text: Hirudo medicinalis

text: EcoRI

text: NotI

text: whole embryo

text: CBBP Hirudo medicinalis hermaphrodite embryo


text: live

text: Generic

text: Male-subtract-Female 3-dpc. embryonic cDNA library, BD biosciences Clontech (cat. #K1804-1)

text: 3 days post-conception embryo

text: Gallus gallus

text: White Leghorn

text: Chicken Male-subtract-Female 3-dpc. embryonic cDNA library


text: Institut National de la Recherche Agronomique

text: live

text: Generic

text: Levovil

text: Library from levovil fruit cDNAs subtracted by cDNAs from LCX fruit at the same stages of development. LCX is a line isogenic to Levovil except for 5 fruit-quality-QTL

text: late maturation (breaker and red ripe)

text: fruit

characteristic: organ

text: Solanum lycopersicum

text: RSA-I

text: RSA-I

text: tomato fruit-quality-QTL subtractive selective hybridisation library

text: plasmid

text: TOPO, invitrogene


text: University of Victoria

text: live

text: Generic

text: Tissue contributors: Ikuo Hirono and Takashi Aoki (Laboratory of Genome Science, Tokyo University of Marine Science and Technoloy)

text: spleen, kidney

text: Plecoglossus altivelis altivelis

characteristic: organism

text: mixed tissue

text: paltayu mixed_tissue Plecoglossus altivelis altivelis cDNA

text: pDNR-LIB


text: University of Victoria

text: live

text: Generic

text: spleen, kidney

text: Plecoglossus altivelis altivelis

text: mixed tissue

text: paltayu2 mixed_tissue Plecoglossus altivelis altivelis cDNA

text: pDNR-LIB


text: Agronomic Institute of Campinas

text: live

text: Generic

text: Citrus limonia

text: root

text: Rangpur lime root, greenhouse plant


text: Agronomic Institute of Campinas

text: live

text: Generic

text: Citrus limonia

text: root

text: Rangpur lime root, plant with hydric stress


text: Agronomic Institute of Campinas

text: live

text: Generic

text: Citrus reticulata

text: leaf

text: Mandarin leaf, greenhouse plant


text: Agronomic Institute of Campinas

text: live

text: Generic

text: Citrus reticulata

text: leaf

text: Mandarin leaf, infected with Xylella fastidiosa (stage 1 of 2)


text: Agronomic Institute of Campinas

text: live

text: Generic

text: Citrus reticulata

text: leaf

text: Mandarin leaf, infected with Xylella fastidiosa (stage 2 of 2)


text: Agronomic Institute of Campinas

text: live

text: Generic

text: Citrus reticulata

text: fruit

text: Mandarin fruit, development stadium (1 of 3)


text: Agronomic Institute of Campinas

text: live

text: Generic

text: Citrus reticulata

text: fruit

text: Mandarin fruit, development stadium (2 of 3)


text: Agronomic Institute of Campinas

text: live

text: Generic

text: Citrus reticulata

text: fruit

text: Mandarin fruit, development stadium (3 of 3)


text: Agronomic Institute of Campinas

text: live

text: Generic

text: Citrus sinensis

text: leaf

text: Sweet orange leaf, infected with Xylella fastidiosa (stage 1 of 2)


text: Agronomic Institute of Campinas

text: live

text: Generic

text: Citrus sinensis

text: leaf

text: Sweet orange leaf, infected with Xylella fastidiosa (stage 2 of 2)


text: Agronomic Institute of Campinas

text: live

text: Generic

text: Citrus sinensis

text: leaf

text: Sweet orange leaf, infected with CiLV


text: Agronomic Institute of Campinas

text: live

text: Generic

text: Citrus sinensis

text: leaf

text: Sweet orange leaf, young greenhouse plant


text: Agronomic Institute of Campinas

text: live

text: Generic

text: Citrus sinensis

text: bark

text: Sweet orange bark, greenhouse plant


text: Agronomic Institute of Campinas

text: live

text: Generic

text: Citrus sinensis

text: fruit

text: Sweet orange fruit, development stadium (1 of 6)


text: Agronomic Institute of Campinas

text: live

text: Generic

text: Citrus sinensis

text: fruit

text: Sweet orange fruit, development stadium (2 of 6)


text: Agronomic Institute of Campinas

text: live

text: Generic

text: Citrus sinensis

text: fruit

text: Sweet orange fruit, development stadium (3 of 6)


text: Agronomic Institute of Campinas

text: live

text: Generic

text: Citrus sinensis

text: fruit

text: Sweet orange fruit, development stadium (4 of 6)


text: Agronomic Institute of Campinas

text: live

text: Generic

text: Citrus sinensis

text: fruit

text: Sweet orange fruit, development stadium (5 of 6)


text: Agronomic Institute of Campinas

text: live

text: Generic

text: Citrus sinensis

text: fruit

text: Sweet orange fruit, development stadium (6 of 6)


text: Agronomic Institute of Campinas

text: live

text: Generic

text: Citrus sinensis

text: flower

text: Sweet orange flower, greenhouse plant


text: Agronomic Institute of Campinas

text: live

text: Generic

text: Citrus sinensis

text: leaf

text: Sweet orange leaf, field plant A


text: Agronomic Institute of Campinas

text: live

text: Generic

text: Citrus sinensis

text: leaf

text: Sweet orange leaf, field plant B


text: Agronomic Institute of Campinas

text: live

text: Generic

text: Citrus trifoliata

text: leaf

text: Poncirus trifoliata leaf, greenhouse plant


text: Agronomic Institute of Campinas

text: live

text: Generic

text: Citrus trifoliata

text: leaf

text: Poncirus trifoliata leaf, infected with CTV


text: Agronomic Institute of Campinas

text: live

text: Generic

text: Citrus trifoliata

text: bark

text: Poncirus trifoliata bark, greenhouse plant


text: Agronomic Institute of Campinas

text: live

text: Generic

text: Citrus trifoliata

text: bark

text: Poncirus trifoliata bark, infected with Phytophthora parasitica


text: Agronomic Institute of Campinas

text: live

text: Generic

text: Citrus trifoliata

text: seed

text: Poncirus trifoliata seed, field plant


text: Agronomic Institute of Campinas

text: live

text: Generic

text: Citrus sunki

text: bark

text: Sunki mandarin bark, greenhouse plant


text: University of London

text: live

text: Generic

text: Agelena orientalis

text: venom gland

text: Agelena orientalis venom gland


text: University Children's Hospital of Cologne

text: live

text: Generic

text: 3'ESTs converted from SAGE tag sequences using GLGI and 5'RACE

characteristic: dev-stage

text: Homo sapiens

text: primary neuroblastoma

text: Human primary neuroblastoma stage 4

text: Plasmid

text: TopoTA-Vector (Invitrogen)


text: live

text: Generic

text: Homo sapiens

text: primary neuroblastoma

text: Human primary neuroblastoma stage 4S

characteristic: title

text: Plasmid

text: TopoTA-Vector (Invitrogen)


characteristic: External Id

text: Universite Lille1

text: live

text: Generic

text: Suppression Subtractive Hybridization was used to enrich for transcripts either up or down regulated after metallic exposure. The library was prepared using protocol given by supplier (Clontech)

text: Adults

text: Eisenia fetida

text: hermaphrodite

characteristic: sex

text: Ef

text: Coelomocytes

text: Eisenia fetida cDNAs from SSH library

text: Plasmids

text: pCR4 TOPO


text: University of Florida

characteristic: INSDC center name

text: live

text: Generic

text: The 3'anchored cDNA Sequences were generated using the 454-GS20 platform. cDNA was prepared from total RNA using a biotinylated dT-B adaptor to prime first strand synthesis. Double stranded cDNA was purified on streptavidin beads and digested with MspI. A custom A-adaptor that incorporated a 3 base multiplex key a CG overhang was ligated to the MspI ends. Following ligation, single stranded sequencing template was eluted and sequenced on the GS-20 instrument. Libraries were constructed from wild type and viviparous1 mutant ovaries and sequenced in a single multiplex reaction. Consensus sequences for anchored cDNA tags were obtained by CAP3 assembly. Additional details are included in the publication.

text: prefertilization

text: Ovary

text: Zea mays

text: MspI

text: W22 wild type and viviparous1 mutant

text: Maize ovary - Eveland 2007


text: South Australian Research and Development Institute

text: live

text: Generic

text: Abaxial epidermis was peeled off 24 hours after inoculation with Pyrenophora teres f. teres and Pyrenophora teres f. maculata. Suppression subtractive hybridizations were carried out using cDNA populations from incompatible and compatible interactions.

text: Primary leaves

text: Hordeum vulgare subsp. vulgare

text: Epidermis

text: Subtracted libraries from epidermis of 10 day old barley seedlings inoculated with Pyrenophora teres


text: NRC Institute for Marine Biosciences Industrial Partnership Facility

text: live

text: Generic

text: Gadus morhua

text: testis

text: Gadus morhua testis library


text: live

text: Generic

text: Gadus morhua

text: brain

text: Gadus morhua brain SSH library enriched for genes up-regulated by polyriboinosinic polyribocytidylic acid (polyI:C)


text: live

text: Generic

text: Gadus morhua

text: brain

text: Gadus morhua brain SSH library enriched for genes down-regulated by polyriboinosinic polyribocytidylic acid (polyI:C)


text: live

text: Generic

text: Gadus morhua

text: head kidney

text: Gadus morhua head kidney SSH library enriched for genes up-regulated by formalin-killed atypical Aeromonas salmonicida


text: live

text: Generic

text: Gadus morhua

text: head kidney

text: Gadus morhua head kidney SSH library enriched for genes down-regulated by formalin-killed atypical Aeromonas salmonicida


text: live

text: Generic

text: Gadus morhua

text: blood

text: Gadus morhua blood SSH library enriched for genes up-regulated by formalin-killed atypical Aeromonas salmonicida


text: live

text: Generic

text: Gadus morhua

text: blood

text: Gadus morhua blood SSH library enriched for genes down-regulated by formalin-killed atypical Aeromonas salmonicida


text: live

text: Generic

text: Gadus morhua

text: spleen

text: Gadus morhua spleen SSH library enriched for genes up-regulated by formalin-killed atypical Aeromonas salmonicida


text: live

text: Generic

text: Gadus morhua

text: spleen

text: Gadus morhua spleen SSH library enriched for genes down-regulated by formalin-killed atypical Aeromonas salmonicida


text: The Institute for Genomic Research

text: live

text: Generic

text: Constructed by Bento Soares at the Department of Psychiatry, Columbia University. Directionally cloned 5'->3' into the HindIII->NotI sites of the lafmid A1 vector.

text: Homo sapiens

text: HindIII

text: NotI

text: Fetal Brain, Bento Soares

text: Lafmid A1

characteristic: vector


text: The Institute for Genomic Research

characteristic: INSDC first public

text: live

text: Generic

text: 17-18 wk gestation, female; oligo-dT + random primed cDNA synthesis; lambdaZAP-II vector, 1.0kb average inser size.

text: Homo sapiens

text: Fetal brain, Stratagene (cat#936206)

text: Phage

text: LambdaZAP-II


text: The Institute for Genomic Research

text: live

text: Generic

text: mRNA was purified from the hippocampus of an adult female. cDNA was constructed and cloned simultaneously using vector priming with the E145 vector and method described by Rubenstein, et. al (Nucl. Acids Res. 18:4833, 1990). cDNA was directionally synthesized from the NotI site in the vector to the HindIII site.

text: Homo sapiens

text: NotI

text: HindIII

text: Hippocampus, Ruben Moreno

text: Phage


text: The Institute for Genomic Research

text: live

text: Generic

text: The hippocampus library (#4 above) was subtracted with a fibroblast cell line cDNA library (Stratagene cat. #936209; WI38 lung fibroblast cell line; oligo-dT + random primed cDNA synthesis; lambdaZAP-II vector, 1.0kb average insert size.) by the method of Sive & St. John (Nucl. Acids Res. 16:10937, 1988).

text: Homo sapiens

text: Subtracted Hippocampus, Stratagene (cat. #936205)

text: Phage

text: lambdaZAP-II


text: The Institute for Genomic Research

text: live

text: Generic

text: Female, 2 years; oligo-dT primed cDNA synthesis; lambdaZAP vector, 1.5kb average insert size. Note: In our hands, this library was ~50% non-recombinant

text: Homo sapiens

text: Temporal Cortex, Stratagene (cat. #935205)

text: Phage

text: lambdaZAP


text: The Institute for Genomic Research

text: live

text: Generic

text: mRNA from 'Adult brain' was purchased from Clontech (cat. #6516-1). cDNA was directionally cloned in the Rubenstein vector as described by Rubenstein, et. al (Nucl. Acids Res. 18:4833, 1990).

text: Homo sapiens

text: NotI

text: HindIII

text: Whole Brain, Clontech mRNA, Ruben Moreno

text: Phage


text: University of Central Florida

text: live

text: Generic

text: PolyA+ RNA, from asynchronous blood stage parasites of the cloned Honduran HB3 isolate cultured in vitro, was reverse transcribed using an oligo dT-Xho I primer. Second strand was prepared using RNase H and DNA polymerase I. EcoR I adapters were ligated to the cDNA ,and it was cleaved with Xho I. Prepared fragments were ligated into EcoR I + Xho I double-digested pBlueScript SK(+), and transformed E. coli XL1-Blue.

text: E. coli XL1-Blue

characteristic: lab-host

text: Plasmodium falciparum

text: cbsPfHB3.1, Debopam Chakrabarti

text: Plasmid

text: pBlueScript SK(+)


text: University of Florida

characteristic: INSDC last update

text: live

text: Generic

text: Genomic DNA, from asynchronous blood stage parasites of the cloned Honduran HB3 isolate cultured in vitro, was digested with mung bean nuclease in the presence of 30% formamide at 50oC (Vernick, K.D., Imberski, R.B., and McCutchan, T.F. 1988. Nucleic Acids Research 16:6883-6896). The ends of the fragments were polished using T4 DNA polymerase, and the fragments were ligated to EcoR V-cleaved and dephosphorylated pBlueScript SK(+). Recombinant plasmids transformed E. coli XL1-Blue.

text: E. coli XL1-Blue

text: Plasmodium falciparum

text: gmbPfHB3.1, G. Roman Reddy

text: Plasmid

text: pBlueScript SK(+)


text: The Institute for Genomic Research

text: live

text: Generic

text: C. elegans mixed stage cDNA library. Strategene catalog #937006. The library is oligo dT primed and directionally cloned in the Uni-ZAP XR vector.

text: Caenorhabditis elegans

text: Mixed stage, Stratagene (cat. #937006)

text: Uni-ZAP XR


text: Genethon Centre de recherche sur le Genome Humain

text: live

text: Generic

text: T Lymphoblastoid cell line ATCC#CCL119

characteristic: cell-line

text: T Lymphoblastoid cell line ATCC#CCL119. pBluescript SK(+) cloning vector.

text: Homo sapiens

text: T lymphoblast

text: CLONTECH cDNA library CCRF-CEM, cat# HL1063g

text: Plasmid

text: pBluescript SK(+)


text: (USA) Washington Univ. School of Medicine or (UK) MRC Laboratory of Molecular Biology

text: live

text: Generic

text: Mixed stage hermaphrodite cDNA library. Partially normalized by successively picking groups of clones that didn't hybridize to previously picked clones. Vector: lambdaphage SHLX2 (Lipshitz, D.H. et al., Gene 88:25-36 (1990)) Host: MC1061

text: Caenorhabditis elegans

text: Bristol N2

characteristic: strain

text: Chris Martin sorted cDNA library

text: Phage

text: lambdaphage SHLX2


text: Karolinska Institutet

text: live

characteristic: INSDC status

text: Generic

text: Testis tissue. Differential cDNA screening strategy used.

text: Mus musculus

text: BALB/c

text: Lambda uni-ZAP II

text: Phage

text: Lambda uni-ZAP II


text: University of Colorado Health Sciences Center

text: live

text: Generic

characteristic: NCBI submission model

text: The FB library (catalog #937201, Stratagene) was constructed by directional cloning and oligo d(T)-priming in Lambda ZAP II phage utilizing mRNA from a 17 week human fetus (total brain).

text: Homo sapiens

text: Fetal brain, Stratagene

text: Phage

text: Lambda ZAP II


text: live

text: Generic

characteristic: NCBI submission package

text: The IB library was constructed by directional cloning and oligo(dT)-priming in the Lafmid vector, utilizing a three month old infant human brain (total brain).

text: E.coli DH5alpha

text: Homo sapiens

text: Human Infant Brain, Bento Soares

text: Lafmid


text: live

text: Generic

text: Homo sapiens

text: Clontech cerebral cortex, Clontech (cat. #HL1143x)


text: The Institute for Genomic Research

text: live

text: Generic

text: Homo sapiens

text: Hippocampus, prescreened, Stratagene (cat#936205)


text: The Institute for Genomic Research

text: live

text: Generic

text: Homo sapiens

text: Hippocampus, random, Stratagene (cat#936205)


text: The Institute for Genomic Research

text: live

text: Generic

text: Homo sapiens

text: Hippocampus, subtracted, Stratagene (cat#936205)


text: live

text: Generic

text: Homo sapiens

text: Batten's subtracted, Ruben Moreno


text: The Institute for Genomic Research

text: live

text: Generic

text: Homo sapiens

text: Infant Brain, Bento Soares


text: Kellogg Eye Center, University of Michigan

text: live

text: Generic

text: Homo sapiens

text: Subtracted human retinal pigment epithelium (RPE)


text: ISV - UPR40, CNRS

text: live

text: Generic

text: cycling cells

text: Arabidopsis thaliana

text: ecotype Columbia

text: cell suspension culture

characteristic: tissue-type

text: Phage

text: Lambda ZAPII


text: Universite de Perpignan

text: live

text: Generic

text: tissue=Green siliques of A.thaliana ecotype columbia; clone_library=Gif-SeedA+B; Cloning vector: Lambda ZAPII non-oriented.

characteristic: description

text: Arabidopsis thaliana

text: ecotype Columbia

text: Gif-SeedA+B

text: Phage

text: Lambda ZAPII


text: Laboratorium fuer molekulare Biologie

text: live

text: Generic

text: Human heart cDNA library. Cloning vector pBluescript SK(+)

text: Homo sapiens

characteristic: organism

text: Stratagene cDNA library Human heart, cat#936208

text: Plasmid

text: pBluescript SK(+)


text: Laboratoire de Biologie Moleculaire vegetale

text: live

text: Generic

text: tissue=Flower buds of A.thaliana ecotype columbia C24; clone_library=Grenoble-B; Cloning vector: Lambda ZAP.

text: Arabidopsis thaliana

text: ecotype Columbia C24

text: Grenoble-B

text: Phage

text: Lambda ZAP


text: Universite de Paris Sud

text: live

text: Generic

text: Arabidopsis thaliana

text: ecotype Columbia

text: green shoots

text: Ors-A

text: Phage

text: Lambda ZAPII


text: Biologie Moleculaire des Plantes - CNRS

text: live

text: Generic

text: tissue=sliced leaves of A.thaliana ecotype columbia; clone_library=Strasbourg-A; Cloning vector: Lambda ZAPII; Physiological condition: leaves strips incubated 2/3/4 days in liquid culture medium.

text: Arabidopsis thaliana

text: ecotype Columbia

text: Strasbourg-A

text: Phage

text: Lambda ZAPII


text: Laboratoire de Biologie Cellulaire

text: live

text: Generic

text: in vitro-grown etiolated seedlings,5 days old

characteristic: dev-stage

text: Arabidopsis thaliana

text: ecotype Columbia

text: whole seedlings

text: Versailles-VB

text: Plasmid

text: pBluescript


text: The Institute for Genomic Research

text: live

text: Generic

text: Caenorhabditis elegans

text: Early embryo, Stratagene (cat. #937007)

characteristic: title


characteristic: External Id

text: Osaka University

text: live

text: Generic

text: hepatocyte

characteristic: cell type

text: 3'-directed MboI

text: E.coli

text: Homo sapiens

text: male

text: liver

text: Liver HepG2 cell line.


text: Clinical Research Centre

characteristic: INSDC center name

text: live

text: Generic

text: clone_library=B, Human Liver tissue; cloning vector is gt11.

text: Homo sapiens

text: B, Human Liver tissue

text: Phage


text: Clinical Research Centre

text: live

text: Generic

text: clone_library=H, Human adult Brain Cortex tissue; cloning vector is gt11.

text: Homo sapiens

text: H, Human adult Brain Cortex tissue

text: Phage

characteristic: vector


text: Clinical Research Centre

characteristic: INSDC first public

text: live

text: Generic

text: clone_library=JG, Human foetal Kidney tissue; cloning vector is Bluescript.

text: Homo sapiens

text: JG, Human foetal Kidney tissue

text: Plasmid

text: Bluescript


text: Clinical Research Centre

text: live

text: Generic

text: clone_library=MB, Mouse Brain Cortex tissue; cloning vector is Bluescript KS II+.

text: Mus musculus

text: MB, Mouse Brain Cortex tissue

text: Plasmid

text: Bluescript KS II+


text: Clinical Research Centre

text: live

text: Generic

text: clone_library=O, Human adult Bone tissue; cloning vector is gt11.

text: Homo sapiens

text: O, Human adult Bone tissue

text: Phage


text: Clinical Research Centre

text: live

text: Generic

text: clone_library=P, Human foetal Brain Whole tissue; cloning vector is Bluescript.

text: Homo sapiens

text: P, Human foetal Brain Whole tissue

text: Plasmid

text: Bluescript


text: Clinical Research Centre

text: live

text: Generic

text: clone_library=S, Human foetal Adrenals tissue; cloning vector is Bluescript.

text: Homo sapiens

text: S, Human foetal Adrenals tissue

text: Plasmid

text: Bluescript


text: Clinical Research Centre

text: live

text: Generic

text: clone_library=T, Human adult Rhabdomyosarcoma cell-line; cloning vector is Lambda Zap.

text: Homo sapiens

text: Phage

text: Lambda Zap


text: Clinical Research Centre

text: live

text: Generic

text: clone_library=TEST1, Human adult Testis tissue; cloning vector is lambda nm1149

text: Homo sapiens

text: TEST1, Human adult Testis tissue

text: Phage

text: lambda nm1149


text: Clinical Research Centre

text: live

text: Generic

text: clone_library=V, Human Placenta tissue; cloning vector is Bluescript KS II+

text: Homo sapiens

text: V, Human Placenta tissue

text: Plasmid

text: Bluescript KS II+


text: Clinical Research Centre

text: live

text: Generic

text: clone_library=W, Human Liver tissue; cloning vector is Bluescript KS II+

text: Homo sapiens

text: W, Human Liver tissue

text: Plasmid

text: Bluescript KS II+


text: Clinical Research Centre

text: live

text: Generic

text: clone_library=X, Human Liver tissue; cloning vector is Bluescript KS II+

text: Homo sapiens

text: X, Human Liver tissue

text: Plasmid

text: Bluescript KS II+


text: Clinical Research Centre

text: live

text: Generic

text: clone_library=Y, Human Placenta tissue; cloning vector is Bluescript KS II+

text: Homo sapiens

text: Y, Human Placenta tissue

text: Plasmid

text: Bluescript KS II+


text: Genethon Centre de recherche sur le Genome Humain

text: live

text: Generic

text: Tissue from female, 19 years old, normal leg muscle. Cloning vector is Lambda ZAPII, in vivo excision from lambda ZAPII to pBluescript SK(+). Genexpress library reference is B.

text: 19 years

text: leg muscle

characteristic: organ

text: Homo sapiens

text: Female

characteristic: sex

text: skeletal muscle

text: STRATAGENE Human skeletal muscle cDNA library, cat. #936215.

text: Phage

text: Lambda ZAPII


text: Laboratorium fuer molekulare Biologie

characteristic: INSDC last update

text: live

text: Generic

text: Genexpress library reference is A.

text: Homo sapiens

text: Stratagene cDNA library Human fibroblast, cat#937212

text: Plasmid

text: pBluescript SK(+)


text: Laboratoire de Biologie Cellulaire

text: live

text: Generic

text: In vitro-grown etiolated seedlings, 5 days old.

text: Arabidopsis thaliana

text: ecotype columbia

characteristic: strain

text: Versailles-VD

text: Plasmid

text: pBluescript


text: Laboratoire de Biologie Moleculaire vegetale

text: live

characteristic: INSDC status

text: Generic

text: tissue=Flower buds of A.thaliana ecotype columbia C24.

text: Arabidopsis thaliana

text: ecotype columbia C24

text: Grenoble-A

text: Phage

text: Lambda ZAP


text: Canadian Genetic Diseases Network

text: live

text: Generic

characteristic: NCBI submission model

text: cDNA from frontal cortex RNA and from a pool prepared from fetal brain, frontal cortex, bone marrow and liver RNA. The cDNAs were hybridized to immobilized isolated yeast artificial chromosomes from the 4p16.3 region. Following washing at high stringency, cDNA segments that had specifically hybridized were eluted, amplified by PCR and cloned. Clones were mapped back to the YACs. Inserts were sequenced using both standard (T3 and T7) and custom primers.

text: Homo sapiens

text: GeneTrack, 4p16.3 JM Rommens


text: Children's Memorial Research Center

text: live

text: Generic

characteristic: NCBI submission package

text: cDNA from polyA+ RNA from human fetal liver + spleen. 20 weeks post-conception

text: E. coli

characteristic: lab-host

text: Homo sapiens

text: Not I

text: EcoR I

text: Plasmid

text: Lafmid K


text: Children's Memorial Research Center

text: live

text: Generic

text: cDNA from polyA+ RNA from human brain. 3-month old neonate.

characteristic: description

text: E. coli

text: Homo sapiens

text: Not I

text: EcoR I

text: Plasmid

text: Lafmid BK


text: Children's Memorial Research Center

text: live

text: Generic

text: cDNA from polyA+ RNA from human fetal liver + spleen. 20 weeks post-conception.

text: E. coli

text: Homo sapiens

characteristic: organism

text: Not I

text: Hind III

text: Plasmid

text: Lafmid A


text: Children's Memorial Research Center

text: live

text: Generic

text: Size-selected cDNA from polyA+ RNA from human brain. 3-month old neonate.

text: E. coli

text: Homo sapiens

text: Not I

text: Hind III

text: Plasmid

text: Lafmid BA


text: Children's Memorial Research Center

text: live

text: Generic

text: Size-selected cDNA from polyA+ RNA from human brain. 3-month old neonate.This library is the result of an attempted normalization of library 4HB3MA.

text: E. coli

text: Homo sapiens

text: Not I

text: Hind III

text: b4HB3MA Cot109+10-Bio

text: Plasmid

text: Lafmid A


text: Children's Memorial Research Center

text: live

text: Generic

text: E. coli

text: Homo sapiens

text: Not I

text: Hind III

text: b4HB3MA Cot109+103+85-Bio

text: Plasmid

text: Lafmid A


text: Children's Memorial Research Center

text: live

text: Generic

text: E. coli

text: Homo sapiens

text: Not I

text: Hind III

text: b4HB3MA Cot109+103-Bio

text: Plasmid

text: Lafmid A


text: Children's Memorial Research Center

text: live

text: Generic

text: E. coli

text: Homo sapiens

text: Not I

text: Hind III

text: b4HB3MA Cot14.5

text: Plasmid

text: Lafmid A


text: Children's Memorial Research Center

text: live

text: Generic

text: E. coli

text: Homo sapiens

text: Not I

text: Hind III

text: b4HB3MA Cot18-Bio

text: Plasmid

text: Lafmid A


text: Children's Memorial Research Center

text: live

text: Generic

text: E. coli

text: Homo sapiens

text: Not I

text: Hind III

text: Plasmid

text: Lafmid BA


text: Children's Memorial Research Center

text: live

text: Generic

text: Size selected cDNA from polyA+ RNA from human brain. 3-month old infant. This library is the result of an attempted normalization of library b4HB3MA

text: E. coli

text: Homo sapiens

text: Not I

text: Hind III

text: Plasmid

text: Lafmid BA


text: Children's Memorial Research Center

text: live

text: Generic

text: cDNA from polyA+ RNA from human brain. 3-month old neonate. This library is the result of attempted normalization of library 4HB3MA.

text: E. coli

text: Homo sapiens

text: Not I

text: Hind III

text: Cot1374Ft 4HB3MA

text: Plasmid

text: Lafmid A


text: Children's Memorial Research Center

text: live

text: Generic

text: Size-selected cDNA from polyA+ RNA from human brain. 3-month old neonate. This library is the result of an attempted normalization of library 4HB3MA.

text: E. coli

text: Homo sapiens

text: Not I

text: Hind III

text: Cot250Ft b4HB3MA

text: Plasmid

text: Lafmid BA


text: Children's Memorial Research Center

text: live

text: Generic

text: E. coli

text: Homo sapiens

text: Not I

text: Hind III

text: Cot274 N4HB3MA

text: Plasmid

text: Lafmid BA


text: live

text: Generic

text: E. coli

text: Homo sapiens

text: H5 cDNA


text: Children's Memorial Research Center

text: live

text: Generic

text: E. coli

text: Homo sapiens

text: Not I

text: Hind III

text: Plasmid

text: Lafmid BA


text: Children's Memorial Research Center

text: live

text: Generic

text: Attempted normalization of library b4HB3MA. Size selected cDNA from polyA+ RNA from human brain. 3-month old neonate.

text: E. coli

text: Homo sapiens

text: Not I

text: Hind III

text: Plasmid

text: Lafmid BA


text: Children's Memorial Research Center

text: live

text: Generic

text: Attempted normalization of library b4HB3MA. Size-selected cDNA from polyA+ RNA from human brain. 3-month old neonate.

text: E. coli

text: Homo sapiens

text: Not I

text: Hind III

text: N-b4HB3MA Cot109

text: Plasmid

text: Lafmid A


text: Children's Memorial Research Center

text: live

text: Generic

text: cDNA from polyA+ RNA from human fetal liver + spleen. 20 weeks post-conception. This library is the result of an attempted normalization of library 3HFLSK20.

text: E. coli

text: Homo sapiens

text: Not I

text: EcoR I

text: Plasmid

text: Lafmid K


text: Children's Memorial Research Center

text: live

text: Generic

text: cDNA from polyA+ RNA from human brain. 3-month old neonate. This library is the result of attempted normalization of library 4HB3MK.

text: E. coli

text: Homo sapiens

text: Not I

text: EcoR I

text: Plasmid

text: Lafmid BK


text: University of Georgia

text: live

text: Generic

text: Lambda PRL2 is a cDNA library derived from equal quantities of 4 pools of mRNA. The mRNA sources were 1) 7 day germinated etiolated seedlings; 2) tissue culture grown roots; 3) staged plants half with 24 hour light cycle, half on 16 hr light, 8 hour dark- rosettes; 4) same plants as 3 but aerial tissue (stems, flowers and siliques. The vector is BRL's lambda Zip-Lox. The cDNA inserts were directionally cloned with Sal-Not arms using oligo dT primed cDNA.

text: Arabidopsis thaliana

text: Sal

text: Not

text: var columbia

text: Phage

text: lambda Zip-Lox


text: Michigan State University

text: live

text: Generic

text: Lambda PRL1 is a cDNA library derived from equal quantities of 4 pools of mRNA. The mRNA sources were 1) 7 day germinated etiolated seedlings; 2) tissue culture grown roots; 3) staged plants half with 24 hour light cycle, half on 16 hr light, 8 hour dark- rosettes; 4) same plants as 3 but aerial tissue (stems, flowers and siliques. The library was made in Novagen's Lambda Shlox-1 with (oligo dT primed) directional inserts cloned between the EcoRI and HindIII sites.

text: Arabidopsis thaliana

text: EcoRI

text: HindIII

text: var columbia

text: Phage

text: Lambda Shlox-1


text: Michigan State University

text: live

text: Generic

text: The AT-NHC library was made in Nam-Hai Chua's lab Rockefeller Univ. from whole plants. This cDNA library was made in Statagene's Lambda Zap using SmaI linkers and EcoRI-SmaI adaptors and cloned into the EcoRI site.

text: Arabidopsis thaliana

text: var columbia

text: AT-NHC

text: Phage

text: Lambda Zap


text: Genzentrum der Universitaet Muenchen

text: live

text: Generic

text: Cloning strategy is unidirectional. Cell line=Human fibroblast WI-38. Genexpress library reference is A.

text: Homo sapiens

text: Stratagene cat#937212 (1992)

text: Phage

text: Lambda ZAP, pBluescript SK(-)


text: Centro de Investigaciones Biologicas

text: live

text: Generic

text: Mus musculus, Swiss strain, fetal ovary tissue.

text: Mus musculus

text: Swiss

text: Mus musculus, fetal ovary


text: live

text: Generic

text: 17 week foetus retina

text: Homo sapiens

text: Stratagene human foetal retina


text: UCLA

text: live

text: Generic

text: Mouse liver library

text: Mus musculus domesticus

text: Mouse liver


text: live

text: Generic

text: Marsupial mammary gland cDNA library

text: Notamacropus eugenii

text: Tammar wallaby mammary gland


text: INRA-CRJ

text: live

text: Generic

text: Mammary gland of Capra hircus (goat)

text: Capra hircus

text: Goat mammary gland


text: laboratoire de Biologie Moleculaire

text: live

text: Generic

text: Physiological conditions: cycling cells one hour post-inoculation with X.campestris pv campestris strain 147 in the presence of cycloheximide 1 micromolar.

text: Arabidopsis thaliana

text: ecotype columbia

text: Phage

text: Lambda ZAPII


text: Children's Memorial Research Center

text: live

text: Generic

text: Size-selected cDNA from polyA+ RNA from human brain. 3-month old neonate.This library is the result of an attempted normalization of library b4HB3MA.

text: E. coli

text: Homo sapiens

text: Not I

text: Hind III

text: b4HB3MA Cot8-HAP-Ft

text: Plasmid

text: Lafmid BA


text: Institute for Molecular and Cellular Regulation, Gunma University

text: live

text: Generic

text: mRNA was prepared from normal adult human islets. cDNA was directionally synthesized from the Xho I in the vector to the EcoRI site. cDNA was size fractionated to remove sequences <1000 bp in size.

text: Homo sapiens

text: Eco RI

text: Xho I

text: Human pancreatic islet

text: Phage

text: Lambda ZAPII


text: Massachusetts General Hospital

text: live

text: Generic

text: Exons were isolated from human chromosome 9 specific cosmids using the method of exon amplification (Proc. Natl. Acad. Sci USA 88:4005-4009, 1991). Exons were cloned into pBluescriptIIKS+ converted to T vector at the EcoRV site.

text: E. Coli DH5a

text: Homo sapiens

text: Chromosome 9 exon

text: Plasmid

text: pBluescriptIIKS+


text: Laboratoire de Biologie Cellulaire

text: live

text: Generic

text: in vitro-grown etiolated seedlings,5 days old

characteristic: dev-stage

text: Arabidopsis thaliana

text: ecotype Columbia

text: whole seedlings

characteristic: tissue-type

text: Versailles-VC

text: Plasmid

text: pBluescript


characteristic: External Id

text: live

text: Generic

text: mRNA was purified by guanidium isothiocyanate from log-phase cells of Thermotoga maritima using media described by Huber et. al., (Arch. Microbiol. 144:324-333). cDNA was prepared using the lambda ZAP kit (Stratagene). RNA was primed with an oligo dT primer and/or a random primer containing an XhoI site in the first direction. After generating the lambda ZAP library, plasmids were excised for double-strand sequencing.

text: SURE (Stratagene)

text: Thermotoga maritima

text: XhoI

text: EcoRI

text: Thermotoga maritima ESTMT library

characteristic: title

text: pBLUESCRIPT II (Stratagene)


text: live

text: Generic

text: mRNA was purified by guanidium isothiocyanate from log-phase cells of Pyrobaculum aerophilum using media described by Huber et. al., (Arch. Microbiol. 144:324-333). cDNA was prepared using the lambda ZAP kit (Stratagene). RNA was primed with an oligo dT primer and/or a random primer containing an XhoI site in the first direction. After generating the lambda ZAP library, plasmids were excised for double-strand sequencing.

text: SURE (Stratagene)

text: Pyrobaculum aerophilum

text: XhoI

text: EcoRI

text: Pyrobaculum aerophilum ESTMT library

text: pBLUESCRIPT II (Stratagene)


text: Harvard Medical School

characteristic: INSDC center name

text: live

text: Generic

text: E.coli Y1090

text: Homo sapiens

text: EcoRI

text: EcoRI

text: Heart

text: Phage

text: Lambda gt11


text: Laboratorium fuer molekulare Biologie

text: live

text: Generic

text: Homo sapiens

text: Atrium cDNA library Human heart