text: Malaysian Palm Oil Board

text: live

text: Generic

text: Total RNA was isolated from oil palm non-embryogenic callus using the aqueous phenol extraction method. After total RNA extraction, poly (A)+ RNA was isolated using oligo-dT cellulose chromatography technique. Directional cDNA library were constructed using the ZAP-cDNA Synthesis Kit and the ZAP-cDNA Gigapack III Gold Cloning Kit (Stratagene). Oil palm non-embryogenic callus cDNAs were cloned and amplified once to produce a library with a titre of 2.83 X 10^9 pfu/ml.

text: Non-Embryogenic

text: Elaeis guineensis

text: EcoRI

text: XhoI

text: Callus

text: CNL (Oil Palm Non-Embryogenic Callus)

text: Plasmid


status: PUBLIC

characteristic: External Id

text: Malaysian Palm Oil Board

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Total RNA was isolated from oil palm non-embryogenic callus using the aqueous phenol extraction method. After total RNA extraction, poly (A)+ RNA was isolated using oligo-dT cellulose chromatography technique. Directional cDNA library were constructed using the ZAP-cDNA Synthesis Kit and the ZAP-cDNA Gigapack III Gold Cloning Kit (Stratagene). Oil palm non-embryogenic callus cDNAs were cloned and amplified once to produce a library with a titre of 4.64 X 10^9 pfu/ml.

characteristic: description

text: Non-Embryogenic

tag: attribute

characteristic: dev-stage

characteristic: lab-host

text: Elaeis guineensis

characteristic: organism

text: EcoRI

text: XhoI

text: Callus

characteristic: tissue-type

text: CNN (Oil Palm Non-Embryogenic Callus)

characteristic: title

text: Plasmid

characteristic: vector


text: Malaysian Palm Oil Board

text: live

text: Generic

text: Total RNA was isolated from oil palm embryoids using the aqueous phenol extraction method. After total RNA extraction, poly (A)+ RNA was isolated using oligo-dT cellulose chromatography technique. Directional cDNA library were constructed using the ZAP-cDNA Synthesis Kit and the ZAP-cDNA Gigapack III Gold Cloning Kit (Stratagene). Oil palm embryoid cDNAs were cloned and amplified once to produce a library with a titre of 4.86 X 10^9 pfu/ml.

text: Proliferating Embryoid

text: Elaeis guineensis

text: EcoRI

text: XhoI

text: Embryoid

text: EB (Oil Palm Embryoid)

text: Plasmid


text: Malaysian Palm Oil Board

text: live

text: Generic

text: Total RNA was isolated from oil palm embryoids using the aqueous phenol extraction method. After total RNA extraction, poly (A)+ RNA was isolated using oligo-dT cellulose chromatography technique. Directional cDNA library were constructed using the ZAP-cDNA Synthesis Kit and the ZAP-cDNA Gigapack III Gold Cloning Kit (Stratagene). Oil palm embryoid cDNAs were cloned and amplified once to produce a library with a titre of 2.93 X 10^9 pfu/ml.

text: Proliferating Embryoid

text: Elaeis guineensis

text: EcoRI

text: XhoI

text: Embryoid

text: EN (Oil Palm Embryoid)

text: Plasmid


text: MPI for Plant Breeding Research

text: live

text: Generic

text: mature appressoria formed on polystyrene

text: Colletotrichum higginsianum

text: IMI 349063A

characteristic: strain

text: mature appressoria

text: Colletotrichum higginsianum mature appressoria IMI 349063A


text: National Institute of Biomedical Innovation

text: live

text: Generic

text: 1st strand cDNA was primed with an oligo(dT) primer [ATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded using specific 5' and 3' primers and amplified by PCR. The PCR cDNA was synthesized product was digested with SfiI and size selection was performed to exclude fragments <1.5kb. The SfiI-digested PCR product was cloned into distinct DraIII sites of pME18S-FL3. XhoI sites just outside the DraIII sites can be used to isolate the cDNA insert. Libraries were constructed by oligo-capping method.

text: 3 years old

text: Macaca fascicularis

text: DraIII (CACTGTGTG)

text: DraIII (CACTGTGTG)

text: male

characteristic: sex

text: spleen

text: macaque spleen cDNA library QspA

text: pME18S-FL3 (Acc.No. AB009864)


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: V8 broth, PLICH, water. The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected >2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed at DOE Joint Genome Institute (Walnut Creek, CA).

text: DH10B T1R

text: Phytophthora capsici

text: SalI

text: NotI

text: Mycelium

text: CBOT Phytophthora capsici LT1534 Mycelium large


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: V8 broth, PLICH, water. The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 1.2-2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed at DOE Joint Genome Institute (Walnut Creek, CA).

text: DH10B T1R

text: Phytophthora capsici

text: SalI

text: NotI

text: Mycelium

text: CBOU Phytophthora capsici LT1534 Mycelium small


text: Departement de Biochimie, Universite de Montreal

text: live

text: Generic

text: Edhazardia aedis

text: Edhazardia aedis Library #1


text: Northeast Forestry University

text: live

text: Generic

text: 2-3 year

text: E-coli

text: Betula platyphylla

text: hermaphrodite

text: cambium

text: cDNA library of cambium of Betula platyphylla Suk

text: PDNR Lib


text: live

text: Generic

text: Total RNA was used for cDNA library construction. Reverse transcription and cDNA normalization used standard procedures. Three rounds of normalization were performed consecutively. The normalized cDNAs were digested, fragments of 200 bp to 600 bp were purified and ligated into the TRV vector construct pGIIITV00.

text: Nicotiana benthamiana

text: leaf

text: Normalized cDNA library in TRV

text: Tobacco Rattle Virus


text: University of New Hampshire

text: live

text: Generic

text: Smooth Muscle

characteristic: cell type

text: Primary Culture, No Sub-Culture

characteristic: cell-line

text: One to three day-old atherosclerosis-resistant Show Racer (SR) pigeons and atherosclerosis-susceptible (spontaneous, non-induced) White Carneau (WC) pigeons were used to prepare primary cultures of aortic smooth muscle cells (SMC). Explants were grown for seven days until they formed a confluent monolayer. Total RNA was extracted (Trizol, Invitrogen), purified, and converted to double-stranded cDNA using the BD SMART PCR cDNA Synthesis Kit (Clontech). Full-length cDNA from each pigeon strain was cut with DpnII (5'GATC) in order to prepare amplicons for comparison by Representational Difference Analysis (RDA). cDNA fragments were subjected to three rounds of PCR-coupled subtractive hybridization and the final difference products were cloned into the BamHI restriction site of the pBluescript SK+ phagemid vector and transformed into XL1 MRF- chemically competent host cells (Stratagene). Transcripts were purified from the host cells by Rolling Circle Amplification (GE Healthcare) and sequenced by the DNAcore facility at the Hubbard Center for Genome Studies, University of New Hampshire. Sequences represent genes that were differentially expressed between the two strains. The SR136 library contains transcripts that were upregulated in SR SMC relative to WC. The corresponding WC library (WC136) contains transcripts that were upregulated in the WC SMC relative to SR136.

text: XL1 MRF-

text: Columba livia

text: BAMHI

text: Mixed

text: Show Racer

text: Descending Aorta

text: pBluescript SK+


characteristic: External Id

text: University of Pavia

characteristic: INSDC center name

text: live

text: Generic

text: Subjected to normalization

text: 30 min to 36 hr embryos

text: Ceratitis capitata

text: EcoRI

text: NotI

text: ISPRA

text: embryos

text: Ceratitis capitata embryo cDNA library


text: University of Pavia

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Subjected to normalization

text: 0 - 8 day old adults

text: head

characteristic: organ

text: Ceratitis capitata

text: EcoRI

text: NotI

text: ISPRA

text: Ceratitis capitata adult head cDNA library


text: The Samuel Roberts Noble Foundation

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: White lupin seeds (Lupinus albus v Lupro 2085) were scarified, germinated in a coarse sand and turface mixture and grown under greenhouse conditions (temperature, light cycle). Roots of white lupin seedlings were harvested at 5, 10, 15 and 20 days post-emergence, immediately frozen in liquid nitrogen and stored at -80oC prior to analysis. Total RNA was extracted from the root tissue using TRI reagent (Molecular Research Center, Cincinnati, OH). Equal amounts of RNA sample from each time point were pooled and the combined total RNA was used for construction of a cDNA library. Roots for protein analysis were grown under the same greenhouse conditions, except that they were harvested at 17 days post emergence. A developing white lupin root cDNA library was prepared from total RNA using the Creator Smart cDNA library construction kit (BD Bioscience, Palo Alto, CA) following the manufacturer'¯s instructions, and was transformed into E. coli. Eight thousand bacterial clones were randomly picked and used for inoculation of liquid culture. Plasmid DNA was purified from the overnight liquid culture and sequenced with the M13 forward primer.

text: Roots of seedlings at 5, 10, 15 and 20 days post-emergence

text: Lupinus albus

text: Sfi I A (GGCCATTACGGCC)

text: Sfi I B (GGCCGCCTCGGCC)

text: root

text: Lupinus albus L. (white lupin) root

text: pDNR_lib (Clontech)


text: Max Planck Institute for Developemental Biology

text: live

text: Generic

text: mixed stage

characteristic: dev-stage

characteristic: lab-host

text: Pristionchus pacificus

text: hermaphrodite

text: California (PS312)

text: whole body


text: University of New Hampshire

text: live

text: Generic

text: Smooth Muscle

text: Primary Culture, No Sub-Culture

text: One to three day-old atherosclerosis-resistant Show Racer (SR) pigeons and atherosclerosis-susceptible (spontaneous, non-induced) White Carneau (WC) pigeons were used to prepare primary cultures of aortic smooth muscle cells (SMC). Explants were grown for seven days until they formed a confluent monolayer. Total RNA was extracted (Trizol, Invitrogen), purified, and converted to double-stranded cDNA using the BD SMART PCR cDNA Synthesis Kit (Clontech). Full-length cDNA from each pigeon strain was cut with DpnII (5'GATC) in order to prepare amplicons for comparison by Representational Difference Analysis (RDA). cDNA fragments were subjected to three rounds of PCR-coupled subtractive hybridization and the final difference products were cloned into the BamHI restriction site of the pBluescript SK+ phagemid vector and transformed into XL1 MRF- chemically competent host cells (Stratagene). Transcripts were purified from the host cells by Rolling Circle Amplification (GE Healthcare) and sequenced by the DNAcore facility at the Hubbard Center for Genome Studies, University of New Hampshire. Sequences represent genes that were differentially expressed between the two strains. The SR143 library contains transcripts that were upregulated in SR SMC relative to WC. The corresponding WC library (WC143) contains transcripts that were upregulated in the WC SMC relative to SR143.

characteristic: description

text: XL1 MRF-

text: Columba livia

characteristic: organism

text: BAMHI

text: Mixed

text: Show Racer

text: Descending Aorta

characteristic: tissue-type

text: pBluescript SK+


text: University of New Hampshire

text: live

text: Generic

text: Smooth Muscle

text: Primary Culture, No Sub-Culture

text: One to three day-old atherosclerosis-resistant Show Racer (SR) pigeons and atherosclerosis-susceptible (spontaneous, non-induced) White Carneau (WC) pigeons were used to prepare primary cultures of aortic smooth muscle cells (SMC). Explants were grown for seven days until they formed a confluent monolayer. Total RNA was extracted (Trizol, Invitrogen), purified, and converted to double-stranded cDNA using the BD SMART PCR cDNA Synthesis Kit (Clontech). Full-length cDNA from each pigeon strain was cut with DpnII (5'GATC) in order to prepare amplicons for comparison by Representational Difference Analysis (RDA). cDNA fragments were subjected to three rounds of PCR-coupled subtractive hybridization and the final difference products were cloned into the BamHI restriction site of the pBluescript SK+ phagemid vector and transformed into XL1 MRF- chemically competent host cells (Stratagene). Transcripts were purified from the host cells by Rolling Circle Amplification (GE Healthcare) and sequenced by the DNAcore facility at the Hubbard Center for Genome Studies, University of New Hampshire. Sequences represent genes that were differentially expressed between the two strains. The SR225 library contains transcripts that were upregulated in SR SMC relative to WC. The corresponding WC library (WC225) contains transcripts that were upregulated in the WC SMC relative to SR225.

text: XL1 MRF-

text: Columba livia

text: BAMHI

text: Mixed

text: Show Racer

characteristic: strain

text: Descending Aorta

characteristic: title

text: pBluescript SK+

characteristic: vector


text: CIDEIM

text: live

text: Generic

text: Aedes aegypti

text: midgut

text: Aedes aegypti midgut library


text: Qingdao Agricultural University

text: live

text: Generic

text: Cucumis sativus

text: Leaf

text: Salicylic acid induced cDNA of chilling stress cucumber leaves


text: Max Planck Institute of Molecular Plant Physiology

text: live

text: Generic

text: hookeri de Vries

characteristic: cultivar

text: SUPERSCRIPT Plasmid System (Invitrogen, Carlsbad, CA, USA)

text: two youngest leaves of 9-week-old plants

text: leaf

text: Oenothera elata subsp. hookeri

text: NotI

text: SalI

text: Oenothera strain hookeri de Vries leaf library

text: Plasmid


characteristic: External Id

text: Embrapa Recursos Geneticos e Biotecnologia

text: live

text: Generic

text: Prata Ligeiro

text: A subtractive cDNA library of root tissue was constructed using the PCR Select Subtraction Kit (Clontech, USA). Subtractive hybridizations were performed using cDNA from the stressed plant roots (tester) against cDNA from well watered plant roots (driver). The differential products obtained were cloned into pGEM T-Easy (Promega) and sequenced in ABI Prism 3700 DNA Analyser (Applied Biosystems Inc.). A minimum insert size of 30 pb and at least 20 pb with quality of phred> 20 were considered for the analysis.

text: Reproductive stage

text: E. coli XL1 Blue

text: root

text: Oryza sativa

text: EcoRI

text: SpeI

text: Prata Ligeiro drought stressed roots (RAPLIG)

text: Plasmid

text: pGEM-T Easy Vector


characteristic: INSDC center name

text: live

text: Generic

text: IRAT 20

text: Reproductive stage

text: E. coli XL1 Blue

text: root

text: Oryza sativa

text: EcoRI

text: SpeI

text: IRAT 20 drought stressed roots (RAIRAT)

text: Plasmid

text: pGEM-T Easy Vector


text: University of Helsinki

characteristic: INSDC first public

text: live

text: Generic

text: flowers and inflorescences

text: Betula pendula

text: flowers and inflorescences

text: Birch male and female flowers and inflorescences


text: National University of Singapore

characteristic: INSDC last update

text: live

text: Generic

text: Total RNA and mRNA were isolated and purified following the protocols of the RNeasy Maxi Kit and the Oligotex mRNA Mini Kit, respectively (QIAGEN). The cDNA library was constructed following the protocols of the ZAP-cDNA Synthesis Kit (Stratagene). Plasmids were isolated and inserts end-sequenced by T3 promoter primer. Traces were processed for submission to dbEST. The project was supported by BMRC Grant.

text: Egg, Nymph and adult

text: XLI-blue

text: Whole organism

text: Blattella germanica

text: EcoR I

text: Xho I

text: Male and female

characteristic: sex

text: Whole organism

text: German Cockroach Whole Organism cDNA Library

text: Plasmid

text: pBluescriptSK(-)(Stratagene)


text: National University of Singapore

text: live

characteristic: INSDC status

text: Generic

text: Total RNA and mRNA were isolated and purified following the protocols of the RNeasy Mini Kit (QIAGEN) and the Quick mRNA isolation kit (Stratagene), respectively. The cDNA library was constructed following the protocols of the ZAP-cDNA Synthesis Kit (Stratagene). Plasmids were isolated and inserts end-sequenced by T3 promoter primer. Traces were processed for submission to dbEST.

text: XLI-blue

text: Whole organism

characteristic: organ

text: Curvularia lunata

text: EcoR I

text: Xho I

text: Whole organism

text: Curvularia lunata Whole Organism cDNA Library

text: Plasmid

text: pBluescriptSK(-)(Stratagene)


text: National University of Singapore

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Whole organism

text: Periplaneta americana

text: Male and female

text: Whole organism

text: Periplaneta americana Whole Organism cDNA Library

text: Plasmid


text: National University of Singapore

text: live

text: Generic

text: Library was constructed following instruction from Smart cDNA Library Construction Kit (Clontech, USA).

text: All stages

characteristic: dev-stage

text: Local

characteristic: isolate

text: XLI-blue

characteristic: lab-host

text: Whole organism

text: Trichophyton mentagrophytes

text: SfiIA

text: SfiIB

text: Hermaphrodite

text: Whole organism

text: Dermatophytes fungus Trichophyton mentagrophytes EST

text: Plasmid

text: pTriplEx2 (Clontech)


text: Center for the Biology of Nematode Parasitism, North Carolina State University

text: live

text: Generic

text: Hicks Broadleaf

text: expanded leaf primary cluster

characteristic: description

text: Nicotiana tabacum

characteristic: organism

text: leaf

characteristic: tissue-type


text: live

text: Generic

text: Hicks Broadleaf

text: Root Normalized Cluster

text: Nicotiana tabacum

text: Root

characteristic: title


text: live

text: Generic

text: Hicks Broadleaf

characteristic: cultivar

text: Root Primary Cluster

text: Nicotiana tabacum

text: Root


characteristic: External Id

text: University of New Hampshire

text: live

text: Generic

text: Smooth Muscle

characteristic: cell type

text: Primary Culture, No Sub-Culture

characteristic: cell-line

text: One to three day-old atherosclerosis-resistant Show Racer (SR) pigeons and atherosclerosis-susceptible (spontaneous, non-induced) White Carneau (WC) pigeons were used to prepare primary cultures of aortic smooth muscle cells (SMC). Explants were grown for seven days until they formed a confluent monolayer. Total RNA was extracted (Trizol, Invitrogen), purified, and converted to double-stranded cDNA using the BD SMART PCR cDNA Synthesis Kit (Clontech). Full-length cDNA from each pigeon strain was cut with DpnII (5'GATC) in order to prepare amplicons for comparison by Representational Difference Analysis (RDA). cDNA fragments were subjected to three rounds of PCR-coupled subtractive hybridization and the final difference products were cloned into the BamHI restriction site of the pBluescript SK+ phagemid vector and transformed into XL1 MRF- chemically competent host cells (Stratagene). Transcripts were purified from the host cells by Rolling Circle Amplification (GE Healthcare) and sequenced by the DNAcore facility at the Hubbard Center for Genome Studies, University of New Hampshire. Sequences represent genes that were differentially expressed between the two strains. The SR224 library contains transcripts that were upregulated in SR SMC relative to WC. The corresponding WC library (WC224) contains transcripts that were upregulated in the WC SMC relative to SR224.

text: XL1 MRF-

text: Columba livia

text: BAMHI

text: Mixed

text: Show Racer

characteristic: strain

text: Descending Aorta

text: pBluescript SK+

characteristic: vector


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Hicks Broadleaf

text: Normalized Cluster of three tissue types (Root, Flower, Leaf)

text: Nicotiana tabacum

text: Pooled root, flower, leaf


text: Northwest A & F University

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: aborted floral bud

characteristic: organ

text: Brassica rapa subsp. pekinensis

text: aborted floral bud

text: aborted floral bud of chinese cabbage


text: Genetic Engineering and Biotechnology Inst.

text: live

text: Generic

text: Atay 85

text: Four week -old abscisic acid (ABA) teated and untreated wheat plants were harvested. Total RNA was isolated from 50 uM ABA treated and untreated plants. After RT-PCR, 3 different one-base anchored oligo dT primers were used in combination with series of arbitrary 13 mers for mRNA DD reactions. Amplified products were loaded on polyacrylamide gel and differentially expressed transcripts were isolated and amplified by PCR. PCR products were cloned to PCR-TRAP vector, digested with HindIII for the confirmation of the positive clones and then sequenced.

characteristic: description

text: 30 day-old

characteristic: dev-stage

text: E.coli DH5

characteristic: lab-host

text: Triticum aestivum

text: HindIII

text: HindIII

text: Hermaphrodite

characteristic: sex

text: Leaf

text: ABA treated Bread wheat (T.aestivum L.)leaves

text: Plasmid

text: PCR-TRAP


text: Centre for DNA Fingerprinting and Diagnostics

text: live

text: Generic

text: Directional cDNA library was constructed by cloning cDNA fragments into pBluescript II SK(+) vector and sequenced from 5' end

text: Fifth instar larva

text: Antheraea assamensis

characteristic: organism

text: mixed

text: Brain

characteristic: tissue-type

text: Antheraea assama fifth instar larval brain cDNA library

characteristic: title


characteristic: External Id

text: live

text: Generic

text: Fifth instar larva

text: Antheraea assamensis

text: mixed

text: Compound eye

text: Antheraea assama fifth instar larval compound eye cDNA library


text: live

text: Generic

text: Fifth instar larva

text: Antheraea assamensis

text: mixed

text: Epidermis

text: Antheraea assama fifth instar larval epidermis cDNA library


text: live

text: Generic

text: Fifth instar larva

text: Antheraea assamensis

text: mixed

text: Fat body

text: Antheraea assama fifth instar larval fat body cDNA library


text: live

text: Generic

text: Fifth instar larva

text: Antheraea assamensis

text: mixed

text: Midgut

text: Antheraea assama fifth instar larval midgut cDNA library


text: live

text: Generic

text: Fifth instar larva

text: Antheraea assamensis

text: mixed

text: Middle silkgland

text: Antheraea assama fifth instar larval middle silkgland cDNA library


text: live

text: Generic

text: Fifth instar larva

text: Antheraea assamensis

text: female

text: Ovary

text: Antheraea assama fifth instar larval ovary cDNA library


text: live

text: Generic

text: Fifth instar larva

text: Antheraea assamensis

text: mixed

text: Posterior silkgland

text: Antheraea assama fifth instar larval posterior silkgland cDNA library


text: live

text: Generic

text: Fifth instar larva

text: Antheraea assamensis

text: male

text: Testis

text: Antheraea assama fifth instar larval testis cDNA library


text: Genoscope - Centre National de Sequencage

text: live

text: Generic

characteristic: cell type

text: The cDNA library was constructed from total RNA using the CloneMiner cDNA library construction kit (Invitrogen). Growth conditions: 48 hour dark-adapted cells exposed to blue light for 1 hour.

text: Phaeodactylum tricornutum

text: Pt1 8.6, CCMP 2561

characteristic: strain

text: Phaeodactylum tricornutum 48 hour dark-adapted cells exposed to blue light for 1 hour


characteristic: INSDC center name

text: live

text: Generic

text: The cDNA library was constructed from total RNA using the CloneMiner cDNA library construction kit (Invitrogen). Growth conditions: Aerated culture of predominantly triradiate cells.

text: Phaeodactylum tricornutum

text: Pt8, CCMP 2560

text: Phaeodactylum tricornutum Aerated culture of predominantly triradiate cells

characteristic: vector


characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: The cDNA library was constructed from total RNA using the CloneMiner cDNA library construction kit (Invitrogen). Growth conditions: Chemostat culture exposed to 230 micromoles/kg CO2 for 24 hours (20x present-day CO2 levels).

text: Phaeodactylum tricornutum

text: Pt1 8.6, CCMP 2561

text: Phaeodactylum tricornutum Chemostat culture exposed to CO2 for 24 hours


text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: The cDNA library was constructed from total RNA using the CloneMiner cDNA library construction kit (Invitrogen). Growth conditions: Chemostat culture exposed to 230 micromoles/kg CO2 for 4 days (20x present-day CO2 levels).

text: Phaeodactylum tricornutum

text: Pt1 8.6, CCMP 2561

text: Phaeodactylum tricornutum Chemostat culture exposed to CO2 for 4 days


text: University of California-Davis

text: live

text: Generic

characteristic: NCBI submission package

text: Cucurbita maxima

text: phloem

text: Cucurbita maxima (winter squash) phloem


text: University of Copenhagen, Faculty of Life Sciences

text: live

text: Generic

text: 35 days after infection

text: Brassica rapa subsp. pekinensis

text: clubbed root tissue

characteristic: organ

text: Plasmodiophora brassicae

text: P. brassicae, 35 dai


text: MIPS

text: live

text: Generic

text: adult

characteristic: dev-stage

text: Pongo abelii

text: SfilA

text: SfilB

text: liver

text: 470 (synonym: pliv1)


text: MIPS

text: live

text: Generic

text: adult

characteristic: lab-host

text: Pongo abelii

text: SfilA

text: SfilB

text: cortex

text: 459 (synonym: pcor1)


text: MIPS

text: live

text: Generic

text: adult

text: Pongo abelii

text: SfilA

text: SfilB

text: heart

text: 468 (synonym: phrt1)


text: MIPS

text: live

text: Generic

text: adult

text: Pongo abelii

text: SfilA

text: SfilB

text: kidney

text: 469 (synonym: pkid1)


text: University of Agricultural Science, GKVK, Bangalore

text: live

text: Generic

text: cracking type

characteristic: cultivar

text: PCR product having unique band between cracking and non- Cracking genotype of Jack was reamplified with the primer having EcoRI sites having flanking the amplified region. The PCR product was cloned in pUC18 (Acc No: L09136) at EcoRI site and insert was Confirmed by EcoRI digestion and the positive clone was sequenced. Cloned using Quick LIgation Kit (Chromous Cat# RKT31). Plasmid used was pUC18 (AccNo. L09136). Sequencing was done in Sequencing Laboratory, Chromous Biotech Pvt. Ltd. Bangalore-65, India.

characteristic: description

text: Young leaves

text: Artocarpus heterophyllus

text: EcoRI

text: Young leaves

text: Jackfruit cv. cracking type induced gene


text: National Institute for Basic Biology

text: live

text: Generic

text: adult

text: Oryzias latipes

characteristic: organism

text: mixture of male and female

characteristic: sex

text: Hd-rR

text: brain

characteristic: tissue-type

text: OLBR


characteristic: External Id

text: National Institute for Basic Biology

text: live

text: Generic

text: embryo, ST22

text: Oryzias latipes

text: mixture of male and female

text: Hd-rR

text: whole animal

text: OLEA

characteristic: title


text: National Institute for Basic Biology

characteristic: INSDC center name

text: live

text: Generic

text: embryo, ST35

text: Oryzias latipes

text: mixture of male and female

text: Hd-rR

characteristic: strain

text: whole animal

text: OLEB


text: National Institute for Basic Biology

characteristic: INSDC first public

text: live

text: Generic

text: embryo, ST40

text: Oryzias latipes

text: mixture of male and female

text: Hd-rR

text: whole animal

text: OLEC


text: National Institute for Basic Biology

characteristic: INSDC last update

text: live

text: Generic

text: adult

text: Oryzias latipes

text: female

text: Hd-rR

text: ovary

text: OLOVA


text: National Institute for Basic Biology

text: live

characteristic: INSDC status

text: Generic

text: adult

text: Oryzias latipes

text: male

text: Hd-rR

text: liver

text: OLVL


text: Universidad Nacional de La Plata

text: live

text: Generic

characteristic: NCBI submission model

text: cDNA library using RNA of root hairs of Phaseolus vulgaris (common bean) harvested 24 hours after inoculation with Rhizobium etli

text: root

text: Phaseolus vulgaris

text: root hair

text: root hairs of Phaseolus vulgaris harvested 24 hours after inoculation with Rhizobium etli


text: live

text: Generic

characteristic: NCBI submission package

text: Phyllostachys edulis

text: Bambusa edulis, FSEL


text: Universidade Federal de Santa Catarina (UFSC)

text: live

text: Generic

text: Normalized T. rangeli cDNA, LIT derived epimastigote form, Choachi strain

text: Epimastigote

text: Trypanosoma rangeli

text: Choachi

text: pGEM T-easy clonnig vector

characteristic: vector


text: live

text: Generic

text: T. rangeli cDNA, LIT derived epimastigote form, Choachi strain, profile 3

text: Epimastigote

characteristic: dev-stage

text: Trypanosoma rangeli

text: Choachi

text: pGEM T-easy clonnig vector


text: live

text: Generic

text: T. rangeli cDNA, LIT derived epimastigote form, Choachi strain, profile 5

text: Epimastigote

text: Trypanosoma rangeli

text: Choachi

text: pGEM T-easy clonnig vector


text: live

text: Generic

text: T. rangeli cDNA, LIT derived epimastigote form, Choachi strain, profile 6

text: Epimastigote

text: Trypanosoma rangeli

text: Choachi

text: pGEM T-easy clonnig vector


text: live

text: Generic

text: T. rangeli cDNA, LIT derived epimastigote form, Choachi strain, profile 8

text: Epimastigote

text: Trypanosoma rangeli

text: Choachi

text: pGEM T-easy clonnig vector


text: live

text: Generic

text: T. rangeli cDNA, culture trypomastigote forms, Choachi strain, profile 3

text: Trypomastigote

text: Trypanosoma rangeli

text: Choachi

text: pGEM T-easy clonnig vector


text: live

text: Generic

text: T. rangeli cDNA, culture trypomastigote forms, Choachi strain, profile 5

text: Trypomastigote

text: Trypanosoma rangeli

text: Choachi

text: pGEM T-easy clonnig vector


text: live

text: Generic

text: T. rangeli cDNA, culture trypomastigote forms, Choachi strain, profile 6

text: Trypomastigote

text: Trypanosoma rangeli

text: Choachi

text: pGEM T-easy clonnig vector


text: live

text: Generic

text: T. rangeli cDNA, culture trypomastigote forms, Choachi strain, profile 8

text: Trypomastigote

text: Trypanosoma rangeli

text: Choachi

text: pGEM T-easy clonnig vector


text: live

text: Generic

text: T. rangeli cDNA, LIT derived epimastigote form, SC-58 strain, profile 3

text: Epimastigote

text: Trypanosoma rangeli

text: pGEM T-easy clonnig vector


text: live

text: Generic

text: T. rangeli cDNA, LIT derived epimastigote form, SC-58 strain, profile 5

text: Epimastigote

text: Trypanosoma rangeli

text: pGEM T-easy clonnig vector


text: live

text: Generic

text: T. rangeli cDNA, LIT derived epimastigote form, SC-58 strain, profile 6

text: Epimastigote

text: Trypanosoma rangeli

text: pGEM T-easy clonnig vector


text: live

text: Generic

text: T. rangeli cDNA, LIT derived epimastigote form, SC-58 strain, profile 8

text: Epimastigote

text: Trypanosoma rangeli

text: pGEM T-easy clonnig vector


text: live

text: Generic

text: T. rangeli cDNA, culture trypomastigote forms, SC-58 strain, profile 3

text: Trypomastigote

text: Trypanosoma rangeli

text: pGEM T-easy clonnig vector


text: live

text: Generic

text: T. rangeli cDNA, culture trypomastigote forms, SC-58 strain, profile 5

text: Trypomastigote

text: Trypanosoma rangeli

text: pGEM T-easy clonnig vector


text: live

text: Generic

text: T. rangeli cDNA, culture trypomastigote forms, SC-58 strain, profile 6

text: Trypomastigote

text: Trypanosoma rangeli

text: pGEM T-easy clonnig vector


text: Universidade Federal de Santa Catarina (UFSC)

text: live

text: Generic

text: T. rangeli cDNA, culture trypomastigote forms, SC-58 strain, profile 8

text: Trypomastigote

text: Trypanosoma rangeli

text: pGEM T-easy clonnig vector


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: RNA was pooled from C. zeae-maydis growing on 0.2x PDA 3 and 5 days after inoculation and from cultures grown for 5 days on V8-agar and 0.2x PDA supplemented with 10 mM ammonium phosphate. The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 2-8kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed and sequencing performed at DOE Joint Genome Institute (Walnut Creek, CA).

text: 3-10 day old plate cultures

characteristic: lab-host

text: Cercospora zeae-maydis

text: SalI

text: NotI

text: whole mycelia

text: CBYB Cercospora zeae-maydis cercosporin biosynthesis large-insert


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: RNA was pooled from C. zeae-maydis growing on 0.2x PDA 3 and 5 days after inoculation and from cultures grown for 5 days on V8-agar and 0.2x PDA supplemented with 10 mM ammonium phosphate. The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 0.5-2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed and sequencing performed at DOE Joint Genome Institute (Walnut Creek, CA).

text: 3-10 day old plate cultures

text: Cercospora zeae-maydis

text: SalI

text: NotI

text: whole mycelia

text: CBYC Cercospora zeae-maydis cercosporin biosynthesis small-insert


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: RNA was pooled from cultures after 3 and 5 days of growth in conditions that favored conidiation (V8 agar in constant darkness). The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 2-8kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed and sequencing performed at DOE Joint Genome Institute (Walnut Creek, CA).

text: 3 and 5 day-old plate cultures

text: Cercospora zeae-maydis

text: SalI

text: NotI

text: whole mycelia

text: CBYF Cercospora zeae-maydis conidiation large-insert


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: RNA was pooled from cultures after 3 and 5 days of growth in conditions that favored conidiation (V8 agar in constant darkness). The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 0.5-2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed and sequencing performed at DOE Joint Genome Institute (Walnut Creek, CA).

text: 3 and 5 day-old plate cultures

text: Cercospora zeae-maydis

text: SalI

text: NotI

text: whole mycelia

text: CBYG Cercospora zeae-maydis conidiation small-insert


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: RNA was pooled from cultures after 3 and 5 days of growth in conditions that favored conidiation (V8 agar in constant darkness). The library was made from oligo(dT)-primed cDNA and cloned into vector pDNR-LIB (Clontech Laboratories, Inc., Mountain View, CA) using the SMART system (BD Biosciences, San Jose, CA). To generate the first strand, polyA RNA was primed with an oligo(dT) primer (5'-ATTCTAGAGGCCGAGGCGGCCGACATG(T)30 VN-3', where V = A, G, or C). The second strand was synthesized by PCR using primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' followed by digestion of the SfiI sites. cDNA was size selected from 2-8kb using 1.1% agarose gel electrophoresis, then ligated into pDNR-LIB. Library was created and sequencing performed by the DOE Joint Genome Institute (Walnut Creek, CA).

text: 3 and 5 day-old plate cultures

text: Cercospora zeae-maydis

text: SfiIA

text: SfiIB

text: whole mycelia

text: CCAW Cercospora zeae-maydis conidiation not size selected

text: pDNR-LIB


text: National Institute of Animal Science, Rural Development Administration

text: live

text: Generic

text: Hordeum vulgare


text: University of Padova

text: live

text: Generic

text: Total RNA was isolated following the OMNIZOL protocol. cDNA was prepared using SMART approach and normalized using DNS normalization method. Amplified normalized cDNA was ligated into modified pAL9 vector (Evrogen) and used for E. coli transformation.

text: E. coli

text: Head kidney

characteristic: organ

text: Sparus aurata

text: Seabream Head Kidney Library

text: Plasmid

text: pAL9 vector


text: University of Padova

text: live

text: Generic

text: Mixed

characteristic: cell type

text: Total RNA was isolated from Photobacterium damselae subspecies piscicida treated and untreated sea breams and used to prepare normalized cDNA library.

characteristic: description

text: Spleen

text: Sparus aurata

text: Seabream cDN38 Library


text: Justus-Liebig University of Giessen

text: live

text: Generic

text: subtracted cDNA library

text: adult

text: Epiperipatus biolleyi

characteristic: organism

text: whole animals, Immune challenged

text: Epiperipatus biolleyi immune challenged adult

text: pGEM-T Easy


text: College of Horticulture, Northwest A&F University

text: live

text: Generic

text: Growing stage

text: E.coli XL1-Blue

text: Vitis pseudoreticulata

text: SfiI A and B

text: Young leaf

characteristic: tissue-type

text: Young leaves inoculated with Downy mildew pathogen (Plasmopara viticola) cDNA library of Chinese wild grape

text: Phage

text: Lambda TripIEX2


characteristic: External Id

text: Faculdade de Medicina

text: live

text: Generic

text: Secretory

text: PCR-based cDNA library using SMART cDNA library construction kit from Clontech

text: Salivary gland

text: Rhodnius brethesi

text: Salivary gland

text: Rhodnius brethesi salivary gland cDNA library

text: Lambda TriplEx2


text: Faculdade de Medicina

text: live

text: Generic

text: Secretory

text: Salivary gland

text: Rhodnius robustus

text: Salivary gland

text: Rhodnius robustus salivary gland cDNA library

characteristic: title

text: Lambda TriplEx2


text: IRD (Institut de Recherche pour le Developpement)

characteristic: INSDC center name

text: live

text: Generic

text: Bourbon

characteristic: cultivar

text: Clontech PCR-select cDNA subtraction library; Bourbon-specific (Bourbon as tester and Laurina as driver in the subtraction process)

text: E.coli

text: epicotyl and primary leaves

text: Coffea arabica

text: Coffea arabica cv. Bourbon SSH library (Bourbon vs Laurina subtraction)

text: PGEM-T


characteristic: INSDC first public

text: live

text: Generic

text: Laurina

text: Clontech PCR-select cDNA subtraction library; Laurina-specific (Laurina as tester and Bourbon as driver in the subtraction process)

text: E.coli

text: epicotyl and primary leaves

text: Coffea arabica

text: Coffea arabica cv. Laurina SSH library (Bourbon vs Laurina subtraction)

text: PGEM-T


text: NRC Institute for Marine Biosciences Industrial Partnership Facility

characteristic: INSDC last update

text: live

text: Generic

text: Gadus morhua

text: larvae

text: Gadus morhua larvae library


text: live

characteristic: INSDC status

text: Generic

text: Gadus morhua

text: blood

text: Gadus morhua blood library (injected with formalin-killed Aeromonas salmonicida)


text: live

text: Generic

characteristic: NCBI submission model

text: Gadus morhua

text: blood

text: Gadus morhua blood library (injected with polyriboinosinic polyribocytidylic acid)


text: live

text: Generic

characteristic: NCBI submission package

text: Gadus morhua

text: spleen and head kidney

text: Gadus morhua spleen and head kidney library (injected with polyriboinosinic polyribocytidylic acid)


text: Institute of Genetics and Developmental Biology, Chinese Academy of Science

text: live

text: Generic

text: gametophyte

text: Saccharina japonica

text: EcoRI

text: XhoI

text: female

characteristic: sex

text: Laminaria japonica Lambda Zap Express Library

text: Phage

text: Uni-ZAP@ XR vector

characteristic: vector


text: University of Ottawa

text: live

text: Generic

text: adult

characteristic: dev-stage

text: brain

text: Carassius auratus

text: male and female

text: hypothalamus and telencephalon

text: goldfish brain cDNA library

text: plasmid

text: pCR TOPO 2.1


text: Auburn University

text: live

text: Generic

text: Whole body

characteristic: organ

text: Culex quinquefasciatus

text: EcoR I

text: EcoR I

text: Culex quinquefasciatus subtractive cDNA library

text: Plasmid

text: PCRTm 2.1 TA


text: University of California, Riverside

text: live

text: Generic

text: Mixed tissues (embryo, larvae, pupae, adult males and females) from mixed insecticide resistant and insecticide susceptible strains.

text: Culex quinquefasciatus

text: XhoI

text: Adult Males and Females

text: Johannesburg (JHB)

characteristic: strain

text: Embryo, Larvae, Pupae

text: Culex pipiens quinquefasciatus Mixed Tissue Control Library

text: Phage

text: pBluescript SK(-)


text: University of California, Riverside

text: live

text: Generic

text: Bacteria inoculated mosquitoes over a series of time points post infection (RNA supplied by B. Christensen).

characteristic: description

text: Culex quinquefasciatus

text: XhoI

text: Johannesburg (JHB)

text: Culex pipiens quinquefasciatus Bacteria Infected Library

text: Phage

text: pBluescript SK(-)


text: University of California, Riverside

text: live

text: Generic

text: Culex infected with filarial worms (taken at several days post infection).

text: Culex quinquefasciatus

characteristic: organism

text: XhoI

text: Johannesburg (JHB)

text: Culex pipiens quinquefasciatus Filarial Worm Infected Library

text: Phage

text: pBluescript SK(-)


text: University of California, Riverside

text: live

text: Generic

text: West Nile virus infected mosquitoes over a series of time points post infection (RNA supplied by S. Higgs).

text: Culex quinquefasciatus

text: XhoI

text: Johannesburg (JHB)

text: Culex pipiens quinquefasciatus West Nile Virus Infected Library

text: Phage

text: pBluescript SK(-)


text: University of Notre Dame

text: live

text: Generic

text: adult

text: Culex quinquefasciatus

text: male and female

text: Kuala Lumpur, Malaysia

text: whole body

characteristic: tissue-type

text: Culex EST non-normalized


characteristic: External Id

text: United States Department of Agriculture-Agriculture Research Service

text: live

text: Generic

text: Normalized cDNA library (Express Genomics) from eggs from Panama 95 strain of screwworm. Expressed gene sequences from the New World Screwworm, Cochliomyia hominivorax. Directionally cloned Normalized cDNA library (Express Genomics) from eggs from Panama 95 strain of screwworm. pExpressI was the plasmid vector utilized. NotI and EcoRV are the endonucleases used.

text: egg

text: Cochliomyia hominivorax

text: NotI

text: EcoRV

text: Panama 95

text: New World Screwworm Egg 9261 ESTs

text: plasmid


text: live

text: Generic

text: the mRNA was sent to Express Genomics, they synthesized cDNA from the mRNA and inserted it into a plasmid (pExpress-1) using two restriction enzymes Not I and EcoR V. Library description: Normalized cDNA library (Expressed Genomics) from 2nd instar larvae from Panama 95 strain of screwworm. (directionally cloned Normalized cDNA library) After Express Genomics synthesized the cDNA library, the culture was sent to TIGR for sequencing. Library consist of 9387 ESTs. pExpressI was the plasmid vector utilized. NotI and EcoRV are the endonucleases used.

text: larvae

text: Cochliomyia hominivorax

text: NotI

text: EcoRV

text: Panama 95

text: New World Screwworm Larvae 9387 ESTs

characteristic: title

text: plasmid


characteristic: INSDC center name

text: live

text: Generic

text: Subtracted cDNA library using cDNA from adult female ovary dissected from Babesia bovis infection engorged adult female Rhipcephalus (Boophilus) microplus substracted with uninfected adult ovary from R. microplus. Directionally cloned Normalized cDNA library. pExpressI was the plasmid vector utilized. NotI and EcoRV are the endonucleases used.

text: Engorged Adult

text: Ovary

text: Rhipicephalus microplus

text: NotI

text: EcoRV

text: Female

text: La minita

text: B. bovis infected B. microplus Ovary 4058 ESTs

text: plasmid


text: live

text: Generic

text: Subtracted cDNA library using cDNA from adult female Gut dissected from Babesia bovis infection engorged adult female Rhipcephalus (Boophilus) microplus substracted with uninfected adult Gut from R. microplus. pExpressI was the plasmid vector utilized. NotI and EcoRV are the endonucleases used.

text: Engorged Adult

text: Gut

text: Rhipicephalus microplus

text: NotI

text: EcoRV

text: Female

text: La minita

text: B. bovis infected B. microplus Gut 4077 ESTs

text: plasmid


text: University of Oregon

text: live

text: Generic

text: Total RNA was extracted from whole organisms, checked for quality, then prepared for construction of the cDNA library by Michelle Phillips. Plasmids were isolated and inserts end-sequenced by the Marine DNA Sequencing and Analysis Facility at the Center for Marine Functional Genomic Studies, Mount Desert Island Biological Laboratory (J.N. Forrest, Jr., Director). Traces were processed for submission to dbEST by trace2dbest software (Parkinson, Anthony and Blaxter, unpublished software). The project was supported by NIH Trainee Grant Number NIH 5-T32-GMO7413 (University of Oregon), NSF Grant Number 9984202, MDIBL New Investigator Award (N.B. Terwilliger) and NIH Grant Number P20-RR-16463 from the INBRE Program of the National Center for Research Resources (P.H. Hand, PI).

text: Megalopas, 1st and 2nd instar juveniles and adults

text: Metacarcinus magister

text: Cancer magister Whole Organism


text: HHMI, University of Utah

text: live

text: Generic

text: cDNAs from Neoblast (NB). Clone names suffixed with 'T3' were sequenced from their 5' ends. All other clones were obtained by sequencing from their 3' ends.More detail and other relevant information can be found at http://planaria.neuro.utah.edu

text: Schmidtea mediterranea

text: EcORI

text: XhoI

text: NB

text: Plasmid

text: pBSKt


text: HHMI, University of Utah

text: live

text: Generic

text: cDNAs from Head (H). Clone names suffixed with 'T3' were sequenced from their 5' ends. All other clones were obtained by sequencing from their 3' ends.More detail and other relevant information can be found at http://planaria.neuro.utah.edu

text: Schmidtea mediterranea

text: EcORI

text: XhoI

text: H (Schmidtea mediterranea Head)

text: Plasmid

text: pBSKt


text: HHMI, University of Utah

text: live

text: Generic

text: cDNAs from Head Blastema (HB). Clone names suffixed with 'T3' were sequenced from their 5' ends. All other clones were obtained by sequencing from their 3' ends.More detail and other relevant information can be found at http://planaria.neuro.utah.edu

text: Schmidtea mediterranea

text: EcORI

text: XhoI

text: HB (Schmidtea mediterranea Head Blastema)

text: Plasmid

text: pBSKt


text: Central Tuber Crops Research Institute

text: live

text: Generic

text: Mycelial

characteristic: cell type

text: After 7 days of growth in defined liquid Media (Edward et al., 1987)

text: Colocasia esculenta

characteristic: lab-host

text: Phytophthora colocasiae

text: 98-111 (Sikkim, Assam, India)

text: Mycelium

text: Actin gene of Phytophthora colocasiae

text: pGEM-T


text: Instituto Valenciano de Investigaciones Agrarias (IVIA)

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Roots subjected to water deficit

text: adult

text: Citrus sinensis x Citrus trifoliata

text: hermaphrodite

text: root

text: EHR (Terol)

text: Plasmid


text: The Institute of Physical and Chemical Research (RIKEN)

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: df/df mutant at the Prop1 locus (Ames dwarf homozygous mutant)

text: 14.5 days embryo

text: Mus musculus

text: Rathke's pouches

text: RIKEN full-length enriched mouse cDNA library, Rathke's pouches 14.5 days embryo, df/df mutant


text: NRC Institute for Marine Biosciences Industrial Partnership Facility

text: live

text: Generic

characteristic: NCBI submission package

text: Gadus morhua

text: blood

text: Gadus morhua blood library (injected with polyriboinosinic polyribocytidylic acid and formalin-killed Aeromonas salmonicida)


text: live

text: Generic

text: Gadus morhua

text: gill

text: Gadus morhua gill thermal stress library


text: live

text: Generic

text: Gadus morhua

text: embryo

text: Gadus morhua embryo library


text: University of Florida

text: live

text: Generic

text: Red-eared Slider Turtle liver cDNA generated using the SMART method (Clontech) was cloned into pDNR-Lib (Clontech). The cloning was not directional.

text: Liver

text: Trachemys scripta

text: Juvenile Red-eared Slider Turtle Library (Univ Florida Zoology)


text: University of Florida

text: live

text: Generic

text: Red-eared Slider Turtle whole embryo cDNA generated by subtractive hybridization to enrich for messages expressed in embryos incubated at 26 deg. C and treated with 17beta-estradiol relative to those expressed in embryos incubated under the same conditions and exposed to the vehicle. Cloned into pGEM-T (Promega). The cloning was not directional.

text: Whole Embryo

text: Trachemys scripta

text: Female

characteristic: sex

text: Embryonic Red-eared Slider Turtle Estradiol Subtraction Library (Univ Florida Zoology)


text: University of Florida

text: live

text: Generic

text: Red-eared Slider Turtle whole embryo cDNA generated by subtractive hybridization to enrich for messages expressed in embryos incubated at 31 deg. C relative to those expressed in embryos incubated at 26 deg. C. Cloned into pGEM-T (Promega). The cloning was not directional.

text: Whole Embryo

text: Trachemys scripta

text: Female

text: Embryonic Red-eared Slider Turtle 31 Degree Incubation Subtraction Library (Univ Florida Zoology)


text: University of Florida

text: live

text: Generic

text: Red-eared Slider Turtle whole embryo cDNA generated by subtractive hybridization to enrich for messages expressed in embryos incubated at 26 deg. C relative to those expressed in embryos incubated at 31 deg. C. Cloned into pGEM-T (Promega). The cloning was not directional.

text: Whole Embryo

text: Trachemys scripta

text: Male

text: Embryonic Red-eared Slider Turtle 26 Degree Incubation Subtraction Library (Univ Florida Zoology)


text: University of Perugia

text: live

text: Generic

text: Peripheral blood mononuclear cells

text: This EST was obtained with the cDNA-AFLP method (Cappelli et al Res Vet Sci. 2005 Oct;79(2):105-12). Total RNA was extracted from two horses during a 140 km endurance ride. Blood samples were taken by jugular vein before ride and 0, 24 and 48 h after the competition. The restriction endonucleases EcoRI and MseI were used. Bands that resulted differentially expressed in the time course were isolated from the polyacrilamide gel, cloned in a plasmid vector (Invitrogen PCR4 TOPO) and sequenced.

text: Escherichia coli

text: Equus caballus

text: Blood

text: Endurance Horses PBMC Library

text: Invitrogen PCR4 TOPO

characteristic: vector


text: Institute of Medicinal Plant Development (IMPlaD), Chinese Academy of Medical Sciences and Peking Union Medical College

text: live

text: Generic

text: Zhongchai No.1

characteristic: cultivar

text: CLONTECH SMART cDNA library construction kit

text: in florescence

characteristic: dev-stage

text: E. coli

text: Bupleurum chinense

text: SfiIA

text: SfiIB

text: root

text: Bupleurum chinense DC. full length cDNA library

text: Plasmid

text: pBluescript II SK(-) (sequence between EcoR I and Not I sites replaced by SfiIA and SfiIB adaptor)


text: Universita di Catania, Facolta di Agraria

text: live

text: Generic

text: Subtractive cDNA library was constructed using mRNAs isolated from mycelium of Trichoderma harzianum grown in the presence of cadmium as tester and mRNAs isolated from mycelium of Trichoderma harzianum grown in the presence of mercury as driver

characteristic: description

text: 3 days liquid culture in Czapek Dox medium

text: Trichoderma harzianum

text: SacII

text: SpeI

text: IMI 393899

characteristic: strain

text: mycelium

text: Trichoderma harzianum suppression subtractive hybridization (SSH) cDNA library Hg2+

text: Plasmid

text: pGEM-T Vector


text: Heinrich Heine-Universitat Dusseldorf

text: live

text: Generic

text: cDNA library was developed using SuperScript Plasmid System for cDNA synthesis and plasmid cloning (Gibco-BRL, Eggenstein, Germany) from poly(A)+-RNA of 19 sugar beet genotypes mixed in equal proportion. The corresponding RNAs were isolated from dry mature sugar beet seeds.

text: Beta vulgaris subsp. vulgaris

characteristic: organism

text: NotI

text: SalI

text: pSPORT1 (Gibco-BRL, Eggenstein, Germany)


characteristic: External Id

text: Heinrich Heine-Universitat Dusseldorf

text: live

text: Generic

text: cDNA library was developed using the pBluescript II XR cDNA library construction kit (Stratagene, CA, USA) from sugar beet seeds germinated on pleated filter paper soaked in de-ionized water (1% of germinated seeds).

text: Beta vulgaris subsp. vulgaris

text: EcoR I

text: XhoI

text: pBluescript II SK (+)


text: Heinrich Heine-Universitat Dusseldorf

text: live

text: Generic

text: cDNA library was developed using the pBluescript II XR cDNA library construction kit (Stratagene, CA, USA) from sugar beet seeds germinated on pleated filter paper soaked in de-ionized water at medium germination stage (50% of germinated seeds).

text: Beta vulgaris subsp. vulgaris

text: EcoR I

text: XhoI

characteristic: title

text: pBluescript II SK (+)


text: Heinrich Heine-Universitat Dusseldorf

characteristic: INSDC center name

text: live

text: Generic

text: cDNA library was developed using the pBluescript II XR cDNA library construction kit (Stratagene, CA, USA) from sugar beet seeds germinated on pleated filter paper soaked in de-ionized water at late germination stage (95% of germinated seeds).

text: Beta vulgaris subsp. vulgaris

text: EcoR I

text: XhoI

text: pBluescript II SK (+)


text: UMR 7100 - IFR 85 - ESBS -ULP

text: live

text: Generic

text: Strisselsplat

text: Total RNA exctraction was performed with the RNeasy Plant Mini kit (Qiagen, USA). cDNA was synthesized starting from the 3'-poly(A) of the mRNA using the Smart cDNA synthesis kit (Clontech, USA). The resulting cDNA fragments were cloned into the lambdaZAP-express vector (Stratagene, USA).

text: E.coli TOP10 F'

text: Flower

characteristic: organ

text: Humulus lupulus

text: Female

text: Humulus lupulus Mature Cone cDNA

text: Plasmid

text: lambdaZAP-express


text: UMR 7100 - IFR 85 - ESBS -ULP

text: live

text: Generic

text: Crossbreeding between tetraploid Strisselspalt (female) and Challenger (male)

text: Total RNA was extracted with the phenol/sds method (Sambrook et al., Molecular Cloning, 1989) and purified on a cesium chlorid gradient. cDNA was synthesized starting from the 3'-poly(A) of the mRNA using the Smart cDNA synthesis kit (Clontech, USA). The second strand of the cDNA was synthesized with the FIREPol Taq DNA Polymerase I (Solis Biodyne, Estonia). The resulting cDNA fragments were cloned into a ddTTP tailed pUC19 vector (New England Biolabs, USA).

text: E.coli TOP10 F'

characteristic: lab-host

text: Flower

text: Humulus lupulus

text: HincII

text: HincII

text: Hermaphrodite

text: Humulus lupulus Hermaphrodite flower cDNA

text: Plasmid


text: UMR 7100 - IFR 85 - ESBS -ULP

characteristic: INSDC first public

text: live

text: Generic

text: Wild type

text: Total-RNA extraction was performed with the RNaqueous-4PCR kit (Ambion, USA). cDNA was synthesized starting from the 3'-poly(A) of the mRNA using the Smart cDNA synthesis kit (Clontech, USA). The second strand of the cDNA was synthesized with the FIREPol Taq DNA Polymerase I (Solis Biodyne, Estonia). The resulting cDNA fragments were cloned into a ddTTP tailed pUC19 vector (New England Biolabs, USA).

text: E.coli TOP10 F'

text: Flower

text: Humulus lupulus

text: HincII

text: HincII

text: Male

text: Humulus lupulus Male flower cDNA

text: Plasmid


text: UMR 7100 - IFR 85 - ESBS -ULP

characteristic: INSDC last update

text: live

text: Generic

text: Wild type

text: Total RNA exctraction was performed with the RNaqueous-4PCR kit (Ambion, USA). cDNA was synthesized starting from the 3'-poly(A) of the mRNA using the Smart cDNA synthesis kit (Clontech, USA). The second strand of the cDNA was synthesized with the FIREPol Taq DNA Polymerase I (Solis Biodyne, Estonia). The resulting cDNA fragments were cloned into a ddTTP tailed pUC19 vector (New England Biolabs, USA).

text: E.coli TOP10 F'

text: Seed

text: Humulus lupulus

text: HincII

text: HincII

text: Female

text: Humulus lupulus Seed cDNA

text: Plasmid


text: UMR 7100 - IFR 85 - ESBS -ULP

text: live

characteristic: INSDC status

text: Generic

text: Wild type

text: Total RNA exctraction was performed with the RNeasy Plant Mini kit (Qiagen, USA). cDNA was synthesized starting from the 3'-poly(A) of the mRNA using the Smart cDNA synthesis kit (Clontech, USA). The second strand of the cDNA was synthesized with the FIREPol Taq DNA Polymerase I (Solis Biodyne, Estonia). The resulting cDNA fragments were cloned into a ddTTP tailed pUC19 vector (New England Biolabs, USA).

text: E.coli TOP10 F'

text: Seed

text: Humulus lupulus

text: HincII

text: HincII

text: Female

text: Humulus lupulus Germinated seed cDNA

text: Plasmid


text: UMR 7100 - IFR 85 - ESBS -ULP

text: live

text: Generic

characteristic: NCBI submission model

text: Wild type

text: E.coli TOP10 F'

text: Pollen

text: Humulus lupulus

text: HincII

text: HincII

text: Male

text: Humulus lupulus Germinated pollen cDNA

text: Plasmid


text: UMR 7100 - IFR 85 - ESBS -ULP

text: live

text: Generic

characteristic: NCBI submission package

text: Wild type

text: Total RNA exctraction was performed with the RNaqueous-4PCR kit (Ambion, USA). Poly(A)+ RNA was purified with the Oligo Tex Mini kit (Qiagen, USA). cDNA was synthesized starting from the 3'-poly(A) of the mRNA using the Smart cDNA synthesis kit (Clontech, USA). The subtractive hybridization protocol from BD Biosciences Clontech (Palo Alto, CA) was used to generate the specific cDNA library. Tester cDNA was prepared from wild type male flowers and driver cDNA was from hermaphrodite flowers (Crossbreeding between tetraploid Strisselspalt (female) and Challenger (male)). The resulting cDNA fragments were cloned into a ddTTP tailed pUC19 vector (New England Biolabs, USA).

text: E.coli TOP10 F'

text: Flower

text: Humulus lupulus

text: HincII

text: HincII

text: Male

text: Humulus lupulus Male-Hermaphrodite differential cDNA

text: Plasmid


text: Tamil Nadu Agricultural University

text: live

text: Generic

text: Rice plants (Oryza sativa ssp. Indica cv. Co 43) were grown under green house conditions. Fifty day old plants were inoculated with insects (leaf folder and BPH) and pathogens (R. solani). One day before challenging, a set of plants were treated with PGPR (Pseudomonas fluorescens strain TDK-1). Forty eight hours after inoculation, observations were made on pest/disease development based on the symptoms. Leaf sheaths were collected in liquid nitrogen from the following treatments for RNA isolation. Treatment details: 1. Control 3. Leaf folder alone 5. BPH alone 7. R. solani alone 2. PGPR alone 4. Leaf folder + PGPR 6. BPH + PGPR 8. TDK-1 + R. solani. Total RNA was extracted from the leaf sheath tissues collected from the above treatments using the TRIzol reagent method (Invitrogen, San Diego, USA). DD-RT-PCR experiment was performed to identify the differentially expressed cDNAs during different treatmental conditions using RNAimage system (GeneHunter Corporation, USA). Differentially expressed cDNA fragments were excised from the gel, reamplified and cloned in vector (Fermentas, St. Leon- Rot, Germany). Cloned cDNAs were sequenced by Automated cycle sequencing and raw EST sequences were edited to remove adoptor and vector sequences using the Lucy software programme (Chou and Holmes, 2001). Sequence analysis was performed to identify the putative function of the differentially expressed genes by searching against sequences deposited in NCBI and TIGR databases.

text: 50 days after sowing

text: E. coli

text: Shoot

text: Oryza sativa Indica Group

text: Hind III

text: Hind III

text: Sheath

characteristic: tissue-type

text: Differential genes in Rice plants, BPH challenged Rice cDNA

text: Plasmid (T/ A cloning)

text: pGEMT


text: Tamil Nadu Agricultural University

text: live

text: Generic

text: 50 days after sowing

text: E. coli

text: Shoot

text: Oryza sativa Indica Group

text: Hind III

text: Hind III

text: Sheath

text: Differential genes in Rice plants, LF challenged Rice cDNA

text: Plasmid (T/ A cloning)

text: pGEMT

characteristic: vector


text: Tamil Nadu Agricultural University

text: live

text: Generic

text: 50 days after sowing

text: E. coli

text: Shoot

text: Oryza sativa Indica Group

text: Hind III

text: Hind III

text: Sheath

text: Differential genes in Rice plants, PGPR primed, Leaf folder challenged Rice cDNA

text: Plasmid (T/ A cloning)

text: pGEMT


text: Tamil Nadu Agricultural University

text: live

text: Generic

text: 50 days after sowing

text: E. coli

text: Shoot

text: Oryza sativa Indica Group

text: Hind III

text: Hind III

text: Sheath

text: Differential genes in Rice plants, PGPR strain TDK-1 Primed Rice cDNA

text: Plasmid (T/ A cloning)

text: pGEMT


text: Tamil Nadu Agricultural University

text: live

text: Generic

text: 50 days after sowing

text: E. coli

text: Shoot

text: Oryza sativa Indica Group

text: Hind III

text: Hind III

text: Sheath

text: Differential genes in Rice plants, PGPR strain TDK-1 Primed Rice cDNA challenged with BPH

text: Plasmid (T/ A cloning)

text: pGEMT


text: Tamil Nadu Agricultural University

text: live

text: Generic

text: 50 days after sowing

text: E. coli

text: Shoot

text: Oryza sativa Indica Group

text: Hind III

text: Hind III

text: Sheath

text: Differential genes in Rice plants, PGPR strain TDK-1 Primed and Rhizoctonia solani challenged Rice cDNA

text: Plasmid (T/ A cloning)

text: pGEMT


text: Tamil Nadu Agricultural University

text: live

text: Generic

text: 50 days after sowing

text: E. coli

text: Shoot

text: Oryza sativa Indica Group

text: Hind III

text: Hind III

text: Sheath

text: Differential genes in Rice plants, Rhizoctonia solani challenged Rice cDNA

text: Plasmid (T/ A cloning)

text: pGEMT


text: Tamil Nadu Agricultural University

text: live

text: Generic

text: 50 days after sowing

text: E. coli

text: Shoot

text: Oryza sativa Indica Group

text: Hind III

text: Hind III

text: Sheath

text: Differential genes in Rice plants, Rice control

text: Plasmid (T/ A cloning)

text: pGEMT


text: Ocean University of China

text: live

text: Generic

text: gametophyte

text: Saccharina japonica

text: XhoI

text: female

characteristic: sex

text: Laminaria japonica cDNA library

text: PBluescript SK(+)


text: Institute of Zoonosis, Jilin University

text: live

text: Generic

text: Mixed

characteristic: cell type

text: New born larve within 24 hours

characteristic: dev-stage

text: Trichinella pseudospiralis

text: Male and Female

text: Whole larvae

text: Trichinella pseudospiralis new born larvae cDNA library

text: pBluescript+


text: Teagasc

text: live

text: Generic

text: SSH library generated after vernalization using earlier and later flowering plants as tester and driver respectively.

characteristic: description

text: Lolium perenne

text: Shoot apical meristem

text: pCR 2.1


text: Harvard Medical School

text: live

text: Generic

text: embryonic day 12

text: Gallus gallus

characteristic: organism

text: White Leghorn

characteristic: strain

text: Perichondrium/ Periosteum

text: Embryonic chicken perichondrium/ periosteum library


characteristic: External Id

text: Station biologique de Roscoff UMR 7139, CNRS/UPMC

text: live

text: Generic

text: sporophyte

text: Laminaria digitata

text: thallus

text: Laminaria digitata thallus sporophyte

characteristic: title


text: Cotton Research Institute, CAAS

characteristic: INSDC center name

text: live

text: Generic

text: library preparation by SSH

text: Gossypium arboreum

text: 3-6 leave of cotton seeding

text: (Gossypium arboreum L.) Drought-related cDNA Library of cotton

text: plasmid

text: pGEMT-easy Vector (TaKaRa)


text: Department of Biology, East China Normal University, Shanghai

text: live

text: Generic

text: A non-normalized cDNA library from the hepatopancreas of E. sinensis.

text: adult

characteristic: lab-host

text: Eriocheir sinensis

text: hepatopancreas

text: Eriocheir sinensis hepatopancreas cDNA Library

text: plasmid

text: pBluescriptII


text: Justus-Liebig University of Giessen

characteristic: INSDC first public

text: live

text: Generic

text: subtracted cDNA library

text: second instar larvae

text: Lucilia sericata

text: whole animals, Immune challenged

text: Lucilia sericata immune challenged second instar larvae

text: pGEM-T Easy


text: Oregon State University

characteristic: INSDC last update

text: live

text: Generic

text: adult

text: Anthopleura elegantissima

text: aposymbiotic and symbiotic sea anemone library

text: bacteriophage

text: Lambda Zap II, Stratagene


text: live

characteristic: INSDC status

text: Generic

text: Floral cDNA library of Bambusa edulis

text: Phyllostachys edulis

text: Bambusa edulis, FSEF


text: Institute of Hydrobiology, Chinese Academy of Sciences

text: live

text: Generic

characteristic: NCBI submission model

text: Pinctada imbricata

text: male

text: testis

text: gonad SMART cDNA library of Pinctada martensii


text: University of Basilicata

text: live

text: Generic

characteristic: NCBI submission package

text: Jatropha curcas

text: Leaves

text: cDNA from a phenotypic mutant of Jatropha curcas


text: The Horticulture and Food Research Institute of New Zealand Ltd

text: live

text: Generic

text: Library sequenced by Genesis Research & Development. Library Maker: Faamatala Aliifaalogo. Linker: Linker primer XhoI-poly(dT), adapter EcoRI (AATTCGGCACGAGG)

text: Approximately 13 days after pollination

text: Actinidia deliciosa

text: Small fruit

characteristic: tissue-type

text: (KSFA) A. deliciosa small fruit 13d post pollination

text: pBK-CMV


text: live

text: Generic

text: Ripe fruit, range of ripenesses, vine-ripened

text: Actinidia arguta

text: Fruit

text: (KHFA) A. arguta ripe fruit

text: pBK-CMV

characteristic: vector


text: live

text: Generic

text: Library sequenced by Genesis Research & Development. Library Maker: Ryan Mears. Linker: Linker primer XhoI-poly(dT), adapter EcoRI (AATTCGGCACGAGG)

text: Developing shoot buds from extending water shoots

text: Actinidia deliciosa

text: Shoot bud

text: (KAAA) A. deliciosa developing shoot buds

text: pBK-CMV


text: live

text: Generic

text: Library sequenced by Genesis Research & Development. Library Maker: Faamatala Aliifaalogo. Linker: Linker EcoRI-poly(dT), adapter XhoI (CTCGAGGGCACGAGG)

text: Breaking

text: Actinidia deliciosa

text: Bud

text: (KUBA) Hayward breaking bud

text: pBK-CMV


text: live

text: Generic

text: Young

text: Actinidia eriantha

text: Fruit

text: (KUFA) Actinidia eriantha young fruit

text: pBK-CMV


text: live

text: Generic

text: Library sequenced by Genesis Research & Development. Library Maker: K. Snowden. Linker: Linker primer XhoI-poly(dT), adapter EcoRI (AATTCGGCACGAGG)

text: At anthesis and just after, all stages of petal unfolding

text: Actinidia deliciosa

text: Petal

text: (KABA) A. deliciosa petal

text: pBluescript SK(-)


text: live

text: Generic

text: Ripe fruit and firmness range 2.8-9.1N

text: Actinidia deliciosa

text: Ripe fruit outer cortex

text: (KACA) A. deliciosa ripe fruit outer cortex

text: pBluescript SK(-)


text: live

text: Generic

text: Library sequenced by Genesis Research & Development. Library Maker: Y. Yauk. Linker: Linker primer XhoI-poly(dT), adapter EcoRI (AATTCGGCACGAGG)

text: Ripe fruit, range of fruit firmnesses 2.8-9.1N

text: Actinidia deliciosa

text: Ripe fruit inner cortex

text: (KADA) A. deliciosa ripe fruit inner cortex

text: pBluescript SK(-)


text: live

text: Generic

text: Library sequenced by Genesis Research & Development. Library Maker: Kim Snowden. Linker: Linker primer XhoI-poly(dT), adapter EcoRI (AATTCGGCACGAGG)

text: Dormant buds

text: Actinidia deliciosa

text: Buds

text: (KAEA) Actinidia deliciosa dormant buds before hydrogen cyanamide treatment

text: pBluescript SK(-)


text: live

text: Generic

text: Dormant buds

text: Actinidia deliciosa

text: Buds

text: (KAEB) Actinidia deliciosa dormant buds before hydrogen cyanamide treatment

text: pBluescript SK(-)


text: The Horticulture and Food Research Institute of New Zealand Ltd

text: live

text: Generic

text: Buds 3 days after hicane treatment

text: Actinidia deliciosa

text: Buds

text: (KAFB) Actinidia deliciosa buds 3 days after hydrogen cyanamide treatment

text: pBluescript SK(-)


text: live

text: Generic

text: Mixture of petal developmental stages, volatile profiles also collected

text: Actinidia arguta

text: Petal

text: (KAHA) Actinidia arguta petal

text: pBluescript SK(-)


text: live

text: Generic

text: Library sequenced by Genesis Research & Development. Library Maker: Bart Janssen. Linker: Linker primer NotI-poly(dT), adapter SalI-MluI (TCGACCCACGCGTCCG)

text: Mixture of ripe stages

text: Actinidia chinensis

text: Whole fruit

text: (KAIA) Actinidia chinensis ripe fruit


text: live

text: Generic

text: Dormant buds collected from canes in late winter one day before hydrogen cyanamide treatment

text: Actinidia deliciosa

text: Dormant buds from canes

text: (KAKA) Dormant kiwifruit buds one day before hydrogen cyanamide treatment

text: pBluescript SK(-)


text: live

text: Generic

text: Dormant buds collected from canes in late winter three days after hydrogen cyanamide treatment

text: Actinidia deliciosa

text: Dormant buds from canes

text: (KALA) Dormant kiwifruit buds three days after hydrogen cyanamide treatment

text: pBluescript SK(-)


text: live

text: Generic

text: Ripening fruit just before and just after point of peeling

text: Actinidia eriantha

text: Tissue skin and outer pericarp just below skin

text: (KAJA) Actinidia eriantha ripe fruit skin

text: pBluescript SK(-)


text: live

text: Generic

text: Library sequenced by Genesis Research & Development. Library Maker: Y. Yauk. Linker: Linker primer XhoI-poly(dT), adapter EcoRI (AATTCGGCACGAGG)

text: Canes and secondary shoots

text: Actinidia setosa

text: Stem (not the woody part)

text: (KANA) Actinidia setosa stem

text: pBluescript SK(-)


text: live

text: Generic

text: Library sequenced by Genesis Research & Development. Library Maker: Y. Yauk. Linker: Linker primer NotI-poly(dT), adapter SalI-MluI (TCGACCCACGCGTCCG)

text: Fruit of a range of ripenesses in the edible range (categories 1-5), vine ripened

text: Actinidia arguta

text: Fruit

text: (KAOA) Actinidia arguta ripe fruit


text: live

text: Generic

text: Various stages of opening

text: Actinidia eriantha

text: Male petals

text: (KAPA) Actinidia eriantha petal


text: live

text: Generic

text: Library sequenced by Genesis Research & Development. Library Maker: Faamatala Aliifaalogo (cDNA library KHFA), R. Eckloff (subtracted library KHFB). Linker: Linker primer XhoI-poly(dT), adapter EcoRI (AATTCGGCACGAGG)

text: Ripe fruit, range of ripenesses, vine-ripened

text: Actinidia arguta

text: Fruit

text: (KHFB) A. arguta ripe fruit

text: pBK-CMV


text: live

text: Generic

text: Actinidia arguta

text: Fruit

text: (KAOB) Actinidia arguta ripe fruit


text: live

text: Generic

text: Various stages of opening

text: Actinidia eriantha

text: Male petals

text: (KAPB) Actinidia eriantha petal


text: live

text: Generic

text: Various stages unfolding

text: Actinidia polygama

text: Male petals

text: (KAQA) Actinidia polygama petal


text: live

text: Generic

text: Various stages of unfolding

text: Actinidia polygama

text: Male petals

text: (KAQB) Actinidia polygama petal


text: live

text: Generic

text: Petals at various stages of opening

text: Actinidia arguta

text: Petal

text: (KAHB) Actinidia arguta petal


text: live

text: Generic

text: Petals at various stages of opening

text: Actinidia indochinensis

text: Petals

text: (KATA) Actinidia indochinensis petal


text: live

text: Generic

text: Petals at various stages of opening

text: Actinidia chinensis

text: Petals

text: (KAUA) Actinidia chinensis CK51-05 petal


text: The Horticulture and Food Research Institute of New Zealand Ltd

text: live

text: Generic

text: Exponential stage growth

text: Actinidia chinensis

text: Cell culture

text: (KAVA) Actinidia chinensis cell culture


text: live

text: Generic

text: Petals at various stages of opening

text: Actinidia arguta

text: Petal

text: (KAHC) Actinidia arguta petal


text: live

text: Generic

text: Petals at various stages of opening

text: Actinidia chinensis

text: Petals

text: (KAUB) Actinidia chinensis CK51-05 petal


text: live

text: Generic

text: Exponential stage growth

text: Actinidia chinensis

text: Cell culture

text: (KAVB) Actinidia chinensis cell culture


text: live

text: Generic

text: Three bud samples were collected from treated and untreated vines approximately two weeks after hydrogen cyanamide application, and buds at bud burst from treated vines. mRNA was isolated and pooled (in equal proportions) to make the cDNA. Library sequenced by Genesis Research & Development. Library Maker: Y. Yauk. Linker: Linker primer NotI-poly(dT), adapter SalI-MluI (TCGACCCACGCGTCCG)

text: Actinidia chinensis

text: Breaking buds

text: (KAWA) Actinidia chinensis - active meristems


text: live

text: Generic

text: Actinidia chinensis

text: Breaking buds

text: (KAWB) Actinidia chinensis - active meristems


text: live

text: Generic

text: Three bud samples were collected: from treated and untreated vines approximately two weeks after hydrogen cyanamide application, and buds at bud burst from treated vines. Library sequenced by Genesis Research & Development. Library Maker: Y. Yauk. Linker: Linker primer XhoI-poly(dT), adapter EcoRI (AATTCGGCACGAGG)

text: Actinidia chinensis

text: Breaking buds

text: (KAWC) Actinidia chinensis - active meristems

text: pBluescript SK(-)


text: live

text: Generic

text: One day before and 3 days after hydrogen cyanamide application

text: Actinidia deliciosa

text: Breaking buds

text: (KALB) Kiwifruit transcription factor enriched library (from KALA library)

text: pBluescript SK(-)


text: live

text: Generic

text: Library sequenced by Genesis Research & Development. Library Maker: Yar-Khing Yauk. Linker: Linker primer XhoI-poly(dT), adapter EcoRI (AATTCGGCACGAGG)

text: Exponential stage of growth

text: Actinidia chinensis

text: Cell culture

text: (KAVC) Actinidia chinensis cell culture

text: pBluescript SK(-)


text: live

text: Generic

text: Young leaves, two developmental stages, 15/8/2002 & 1/11/02

text: Actinidia chinensis

text: Leaf

text: (KAYA) Actinidia chinensis young leaf library

text: pBluescript SK(-)


text: live

text: Generic

text: Library sequenced by Genesis Research & Development. Library Maker: Y. Yauk. Linker: Linker primer XhoI-poly(dT), adapter EcoRI (AATTCGGCACGAGG)

text: Ripening fruit just before and just after point of peeling

text: Actinidia eriantha

text: skin and outer pericarp just below skin

text: (KAJB) Actinidia eriantha ripe fruit skin

text: pBluescript SK(-)


text: live

text: Generic

text: Young fruit

text: Actinidia chinensis

text: Fruit

text: (KAZA) Actinidia chinensis young fruit library

text: pBluescript SK(-)


text: live

text: Generic

text: Fruit maturity as tasted and on either side for firmness (range of fruit ripeness)

text: Actinidia chinensis

text: Fruit tissue including skin

text: (KAXA) Subtractive library using two extreme individuals from Actinidia chinensis mapping population

text: pBluescript SK(-)


text: The Horticulture and Food Research Institute of New Zealand Ltd

text: live

text: Generic

text: Library sequenced by Genesis Research & Development.

text: Various stages of opening

text: Actinidia eriantha

text: Petals

text: (KAPC) Actinidia eriantha petal


text: live

text: Generic

text: Library sequenced by Genesis Research & Development. Library Maker: Y. Yauk. Linker: Linker primer NotI-poly(dT), adapter SalI-MluI (TCGACCCACGCGTCCG)

text: Various stages of unfolding

text: Actinidia polygama

text: Male petals

text: (KAQC) Actinidia polygama petal


text: live

text: Generic

text: Actinidia deliciosa

text: Breaking buds

text: (KALC) Kiwifruit transcription factor enriched library (from KALA library)

text: pBluescript SK(-)


text: live

text: Generic

text: Actinidia deliciosa

text: Breaking buds

text: (KALD) Kiwifruit transcription factor enriched library (from KALA library)

text: pBluescript SK(-)


text: live

text: Generic

text: Petals at various stages of opening

text: Actinidia chinensis

text: Petals

text: (KAUC) Actinidia chinensis CK51-05 petal


text: live

text: Generic

text: Library sequenced by Genesis Research & Development. Library Maker: Y. Yauk.

text: Range of fruit ripeness

text: Actinidia chinensis

text: Fruit tissue including skin

text: (KASA) Subtractive library using two extreme individuals from Kiwifruit Mapping population

text: Lambda Uni-ZAP XR û excises to pBluescript SK(-)


text: live

text: Generic

text: Library sequenced by Genesis Research & Development. Library Maker: Rong Mei Wu. Linker: Linker primer XhoI-poly(dT), adapter EcoRI (AATTCGGCACGAGG)

text: Young fruit

text: Actinidia chinensis

text: Fruit

text: (KAZC) Actinidia chinensis young fruit library

text: pBluescript SK(-)


text: live

text: Generic

text: Young leaves, two developmental stages, 15/8/2002 & 1/11/02

text: Actinidia chinensis

text: Leaf

text: (KAYB) Actinidia chinensis young leaf library

text: pBluescript SK(-)


text: live

text: Generic

text: Young fruit

text: Actinidia chinensis

text: Fruit

text: (KAZB) Actinidia chinensis young fruit library

text: pBluescript SK(-)


text: live

text: Generic

text: Range of fruit ripeness

text: Actinidia chinensis

text: Fruit tissue including skin

text: (KASB) Subtractive library using two extreme individuals from Kiwifruit Mapping population


text: live

text: Generic

text: Actinidia chinensis

text: Leaf

text: (KAYC) Actinidia chinensis young leaf library

text: pBluescript SK(-)


text: live

text: Generic

text: Young fruit

text: Actinidia chinensis

text: Fruit

text: (KAZE) Kiwifruit young fruit library

text: pBluescript SK(-)


text: live

text: Generic

text: Young fruit

text: Actinidia chinensis

text: Fruit

text: (KAZD) Actinidia chinensis young fruit library

text: pBluescript SK(-)


text: The Horticulture and Food Research Institute of New Zealand Ltd

text: live

text: Generic

text: Library sequenced by Genesis Research & Development. Library Maker: Yar-Khing Yauk. Linker: Linker primer XhoI-poly(dT), adapter EcoRI (AATTCGGCACGAGG)

text: Root bagged plants, 24

text: Actinidia hemsleyana

text: Thick storage roots, outer suberised epidermis, inner phloem, parenchymatous tissue, musilage containing white flesh, no wood.

text: (KAGA) Actinidia hemsleyana storage roots

text: pBluescript SK(-)


text: live

text: Generic

text: Library sequenced by Genesis Research & Development. Library Maker: Y. Yauk. Linker: Linker primer XhoI-poly(dT), adapter EcoRI (AATTCGGCACGAGG)

text: Root bagged plants 24

text: Actinidia hemsleyana

text: Minor roots of Kaimai, many nematode infected areas

text: (KAMA) Actinidia hemsleyana roots with nematodes

text: pBluescript SK(-)


text: Indiana University, Bloomington

text: live

text: Generic

text: mid third instar larvae, late third instar larvae, and early and late prepupal

text: Onthophagus taurus

text: mixed

characteristic: sex

text: head and thorax

text: Onthophagus taurus larval library


text: Indiana University, Bloomington

text: live

text: Generic

text: 1-4 days after pupation

text: Onthophagus taurus

text: mixed

text: whole body

text: Onthophagus taurus pupal library


text: Universidad de La Frontera

text: live

text: Generic

text: SSH library

text: Deschampsia antarctica

text: SSH ANT/LAB


text: Universidad de La Frontera

text: live

text: Generic

text: SSH library

text: Deschampsia antarctica

text: SSH UV/nonUV


text: CIIMAR, University of Porto

text: live

text: Generic

text: Dicentrarchus labrax

text: liver

text: Liver Sea Bass cDNA library


text: University of Basilicata

text: live

text: Generic

text: Jatropha curcas

text: Leaves

text: cDNA from a phenotypic wild-type of Jatropha curcas


text: RIKILT - Institute of Food Safety

text: live

text: Generic

text: Sante

characteristic: cultivar

text: PVY-inoculated plants and mock-inoculated plants were grown for 12 hours. ClonTech protocol was followed for preparation of the subtraction libraries.

text: 12 hours

text: XL-1 blue ultracompetent cells (Stratagene)

text: Leaf

characteristic: organ

text: Solanum tuberosum

text: Upregulated genes in potato cult. Sante infected by potato virus Y (PVY-NTN)

text: Plasmid

text: pGEM-T easy


text: RIKILT - Institute of Food Safety

text: live

text: Generic

text: Sante

characteristic: description

text: 12 hours

characteristic: dev-stage

text: Leaf

text: Solanum tuberosum

text: Downregulated genes in potato cult. Sante infected by potato virus Y (PVY-NTN)

text: Plasmid

text: pGEM-T easy


text: RIKILT - Institute of Food Safety

text: live

text: Generic

text: Igor

text: 12 hours

text: Leaf

text: Solanum tuberosum

characteristic: organism

text: Upregulated genes in potato cult. Igor infected by potato virus Y (PVY-NTN)

text: Plasmid

text: pGEM-T easy


characteristic: External Id

text: RIKILT - Institute of Food Safety

text: live

text: Generic

text: Igor

text: 12 hours

text: Leaf

text: Solanum tuberosum

text: Downregulated genes in potato cult. Igor infected by potato virus Y (PVY-NTN)

text: Plasmid

text: pGEM-T easy


text: RIKILT - Institute of Food Safety

text: live

text: Generic

text: Desiree

text: Tuber (developing and mature) cDNA clones were subtracted from cDNA clones from plant parts (leaf, stolon, swelling stolon tips) following the ClonTech protocol for preparation of subtraction libraries.

text: Tuber

text: Solanum tuberosum

text: mature tubers, developing tubers

text: Potato tuber cDNA library

characteristic: title

text: Plasmid

text: pGEM-T easy


text: RIKILT - Institute of Food Safety

characteristic: INSDC center name

text: live

text: Generic

text: Desiree

text: cDNA clones from plant parts (leaf, stolon, swelling stolon tips) were subtracted from tuber (developing and mature) cDNA clones following the ClonTech protocol for preparation of subtraction libraries.

text: Plant

text: Solanum tuberosum

text: leaf, stolon, swelling solon tips

text: Mixed potato plant parts cDNA library

text: Plasmid

text: pGEM-T easy


text: RIKILT - Institute of Food Safety

text: live

text: Generic

text: Brodick

text: cDNAs from peel/cortex fractions of halved tubers stored in the dark for 8 days were subtracted cDNA clones from their counterhalves stored under sodium lighting (ca. 7000 lux) for 8 days following the ClonTech protocol for preparation of subtraction libraries.

characteristic: lab-host

text: Tuber

text: Solanum tuberosum

text: Peel, Cortex

text: Potato tubers challenged with stress by exposure to high intensity light (ca. 7000 lux) cDNA library

text: Plasmid

text: pGEM-T easy


text: RIKILT - Institute of Food Safety

characteristic: INSDC first public

text: live

text: Generic

text: Desiree

text: cDNA clones commonly expressed in leaf, stolon, swelling stolon tips, developing and mature tubers.

text: Plant

text: Solanum tuberosum

text: mature tubers, developing tubers, leaf, stolon, swelling solon tips

text: Mixed potato tissues cDNA library

text: Plasmid

text: pGEM-T easy


text: Cotton Research Institute, Chinese Academy of Agricultural Sciences

characteristic: INSDC last update

text: live

text: Generic

text: E.coli

text: Gossypium hirsutum

text: embryonic callus

text: SSH library of differentially expressed genes response to medium changes during cotton somatic embryogenesis

text: Plasmid


text: live

characteristic: INSDC status

text: Generic

text: E.coli

text: Gossypium hirsutum

text: embryonic callus

text: SSH library of cotton somatic embryogenesis

text: Plasmid


text: National Research Council of Canada

text: live

text: Generic

characteristic: NCBI submission model

text: Sequences have been trimmed to remove vector and low quality regions.

text: Brassica napus


text: Genoscope - Centre National de Sequencage

text: live

text: Generic

characteristic: NCBI submission package

text: Podospora anserina

text: S mat+

characteristic: strain

text: Ascospores 20h after germination trigger


text: live

text: Generic

text: Podospora anserina

text: S mat+

text: Young perithecia of less than 48h


text: live

text: Generic

text: Podospora anserina

text: S mat+

text: Perithecia older than 48h


text: live

text: Generic

text: Podospora anserina

text: s

text: Mycelium grown for 48h


text: live

text: Generic

text: Podospora anserina

text: S mat+

text: Rapamycin induced mycelium


text: live

text: Generic

text: Podospora anserina

text: S mat+

text: hetR/hetV incompatible mycelium


text: live

text: Generic

text: Podospora anserina

text: S mat+

text: Senescent mycelium


text: Microbial Evolution Research Group

text: live

text: Generic

text: aggregated and dispersed cells

characteristic: cell type

text: Ministeria vibrans

text: ATCC 50519

text: Ministeria vibrans ATCC 50519


text: Microbial Evolution Research Group

text: live

text: Generic

text: Ministeria vibrans

text: ATCC 50519

text: Ministeria vibrans culture collection ATCC 50519


text: United States Department of Agriculture-Agriculture Research Service

text: live

text: Generic

text: Subtracted cDNA library from Babesia bovis infected Rhipcephalus (Boophilus) microplus larvae, subtracted with uninfected larvae from R. microplus. pExpressI was the plasmid vector utilized. NotI and EcoRV are the endonucleases used.

text: Larval

text: Rhipicephalus microplus

text: La Minita

text: B. bovis infected R. microplus larvae 306 ESTs

text: plasmid

characteristic: vector


text: College of Horticulture, Northwest A&F University

text: live

text: Generic

text: Baihe-35-1; Carignane

characteristic: cultivar

text: A hybrid grape of Vitis pseudoreticulata var. Baihe-35-1 x Vitis vinifera var. Carignane.

text: Growing stage

text: E.coli XL1-Blue

text: Vitis pseudoreticulata x Vitis vinifera

text: SfiI A and B

text: Young leaf

characteristic: tissue-type

text: Young leaves inoculated with powdery mildew pathogen (Uncinula necator) cDNA library of a hybrid grape

text: Phage

text: Lambda TripIEX2


text: National Institute for Plant Genome Research

text: live

text: Generic

text: Xanthi

text: seedling

text: Nicotiana tabacum

text: subtracted cDNA library of CaCIPK6T181/D expressing N. tabacum


text: US Department of Agriculture, Agricultural Research Service

text: live

text: Generic

text: Ogle

text: Developing endosperm

text: Avena sativa

text: XhoI

text: EcoRI

text: Endosperm

text: UMN

text: Plasmid


text: University of Azores

text: live

text: Generic

text: Nematodes were incubated with 1% Galleria menonella larvae hemolymph for 6 hours before RNA was extracted.

characteristic: description

text: Heterorhabditis bacteriophora

text: Heterorhabditis bacteriophora parasite stage library


text: MPI for Molecular Genetics

text: live

text: Generic

text: Total RNA was extracted from 9 specimens of both sex (4 females and 5 males) and weight range 350 g to 4kg. Collected tissue frozen in liq N2 and stored at -80C prior to RNA extraction. mRNA purification and concentration were performed using the Dynal's oligo-dT magnetic beads. Library construction was performed with Creator SMART cDNA library construction kit (Clontech) and the thermostable duplex-specific nuclease (Zhu et al., 2001). Initial LD PCR amplification of 22 cycles for enrichment of full length transcripts was verified on Agarose gels. cDNA sizes (>500 base pairs) were selected using filter columns based on agarose gels as described in the Clontech manual. Library construction and sequencing were performed at the MPI of Molecular Genetics Berlin. isolation_source: aquaculture fish. collection_date:10-Oct-2004. collected_by: Juan Fuentes and Carla Viegas. UNILIB:49125

text: adult

characteristic: dev-stage

text: Sparus aurata

text: mixed

characteristic: sex

text: liver


characteristic: External Id

text: MPI for Molecular Genetics

text: live

text: Generic

text: Total RNA was extracted from 9 specimens of both sex (4 females and 5 males) and weight range 350 g to 4kg. Collected tissue frozen in liq N2 and stored at -80C prior to RNA extraction. mRNA purification and concentration were performed using the Dynal's oligo-dT magnetic beads. Library construction was performed with Creator SMART cDNA library construction kit (Clontech) and the thermostable duplex-specific nuclease (Zhu et al., 2001). Initial LD PCR amplification of 22 cycles for enrichment of full length transcripts was verified on Agarose gels. cDNA sizes (>500 base pairs) were selected using filter columns based on agarose gels as described in the Clontech manual. Library construction and sequencing were performed at the MPI of Molecular Genetics Berlin. isolation_source: aquaculture fish. collection_date: 10-Oct-2004. collected_by: Juan Fuentes and Carla Viegas

text: adult

text: Sparus aurata

characteristic: organism

text: mixed

text: cartilage and bone


text: MPI for Molecular Genetics

characteristic: INSDC center name

text: live

text: Generic

text: adult

text: Sparus aurata

text: mixed

text: heart and vessels

characteristic: title


text: MPI for Molecular Genetics

characteristic: INSDC first public

text: live

text: Generic

text: adult

text: Sparus aurata

text: mixed

text: head kidney


text: MPI for Molecular Genetics

characteristic: INSDC last update

text: live

text: Generic

text: adult

text: Sparus aurata

text: mixed

text: trunk kidney


text: MPI for Molecular Genetics

text: live

characteristic: INSDC status

text: Generic

text: adult

text: Sparus aurata

text: mixed

text: intestine


text: MPI for Molecular Genetics

text: live

text: Generic

characteristic: NCBI submission model

text: adult

text: Sparus aurata

text: mixed

text: muscle


text: MPI for Molecular Genetics

text: live

text: Generic

characteristic: NCBI submission package

text: Total RNA was extracted from 5 male specimens of weight ranging between 400 and 500 g. Collected tissue frozen in liq N2 and stored at -80C prior to RNA extraction. mRNA purification and concentration were performed using the Dynal's oligo-dT magnetic beads. Library construction was performed with Creator SMART cDNA library construction kit (Clontech) and the thermostable duplex-specific nuclease (Zhu et al., 2001). Initial LD PCR amplification of 22 cycles for enrichment of full length transcripts was verified on Agarose gels. cDNA sizes (>500 base pairs) were selected using filter columns based on agarose gels as described in the Clontech manual. Library construction and sequencing were performed at the MPI of Molecular Genetics Berlin. isolation_source: aquaculture fish. collection_date: 10-Oct-2004. collected_by: Juan Fuentes and Carla Viegas

text: adult

text: Dicentrarchus labrax

text: male

text: liver


text: MPI for Molecular Genetics

text: live

text: Generic

text: adult

text: Dicentrarchus labrax

text: male

text: testis


text: MPI for Molecular Genetics

text: live

text: Generic

text: adult

text: Dicentrarchus labrax

text: male

text: brain and pituitary


text: MPI for Molecular Genetics

text: live

text: Generic

text: adult

text: Dicentrarchus labrax

text: male

text: adipose tissue


text: MPI for Molecular Genetics

text: live

text: Generic

text: adult

text: Dicentrarchus labrax

text: male

text: gill


text: MPI for Molecular Genetics

text: live

text: Generic

text: adult

text: Dicentrarchus labrax

text: male

text: spleen


text: MPI for Molecular Genetics

text: live

text: Generic

text: adult

text: Dicentrarchus labrax

text: male

text: skin


text: MPI for Molecular Genetics

text: live

text: Generic

text: adult

text: Sparus aurata

text: mixed

text: skin


text: MPI for Molecular Genetics

text: live

text: Generic

text: Total RNA was extracted from the ovary of a 4 kg female. Collected tissue frozen in liq N2 and stored at -80C prior to RNA extraction. mRNA purification and concentration were performed using the Dynal's oligo-dT magnetic beads. Library construction was performed with Creator SMART cDNA library construction kit (Clontech) and the thermostable duplex-specific nuclease (Zhu et al., 2001). Initial LD PCR amplification of 22 cycles for enrichment of full length transcripts was verified on Agarose gels. cDNA sizes (>500 base pairs) were selected using filter columns based on agarose gels as described in the Clontech manual. Library construction and sequencing were performed at the MPI of Molecular Genetics Berlin. isolation_source: aquaculture fish. collection_date: 10-Oct-2004. collected_by: Juan Fuentes and Carla Viegas

text: adult

text: Dicentrarchus labrax

text: female

text: ovary


text: MPI for Molecular Genetics

text: live

text: Generic

text: adult

text: Dicentrarchus labrax

text: male

text: cartilage and bone


text: MPI for Molecular Genetics

text: live

text: Generic

text: adult

text: Dicentrarchus labrax

text: male

text: heart and vessels


text: MPI for Molecular Genetics

text: live

text: Generic

text: adult

text: Dicentrarchus labrax

text: male

text: head kidney


text: MPI for Molecular Genetics

text: live

text: Generic

text: Total RNA was extracted from 4 female specimens of weight ranging between 350g and 4kg. Collected tissue frozen in liq N2 and stored at -80C prior to RNA extraction. mRNA purification and concentration were performed using the Dynal's oligo-dT magnetic beads. Library construction was performed with Creator SMART cDNA library construction kit (Clontech) and the thermostable duplex-specific nuclease (Zhu et al., 2001). Initial LD PCR amplification of 22 cycles for enrichment of full length transcripts was verified on Agarose gels. cDNA sizes (>500 base pairs) were selected using filter columns based on agarose gels as described in the Clontech manual. Library construction and sequencing were performed at the MPI of Molecular Genetics Berlin. isolation_source: aquaculture fish. collection_date: 10-Oct-2004. collected_by: Juan Fuentes and Carla Viegas

text: adult

text: Sparus aurata

text: female

text: ovary


text: MPI for Molecular Genetics

text: live

text: Generic

text: adult

text: Sparus aurata

text: mixed

text: brain and pituitary


text: MPI for Molecular Genetics

text: live

text: Generic

text: adult

text: Sparus aurata

text: mixed

text: adipose tissue


text: MPI for Molecular Genetics

text: live

text: Generic

text: adult

text: Sparus aurata

text: mixed

text: spleen


text: MPI for Molecular Genetics

text: live

text: Generic

text: adult

text: Sparus aurata

text: mixed

text: gill