text: Purdue University

characteristic: INSDC first public

text: live

text: Generic

text: Fungus was grown on cracked corn. RNA was isolated using phenol LiCl method. PolyA RNA was obtained with Oligotex mRNA spin columns (Qiagen). Subtracted from wild type RNA with PCR select cDNA subtraction kit (Clontech), amplified, cloned into pGEM-TEZ and transformed to E. coli DH5 alpha cells.

text: Fusarium verticillioides

text: EcoRI

text: EcoRI

text: Fusarium verticillioides fcc1 mutant subtraction library

text: plasmid

text: PGem-T-Easy


status: PUBLIC

characteristic: External Id

text: Universidad del Quindio

characteristic: INSDC center name

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: foreskin fibroblast in vitro cultures. Directional cDNA was synthesized by oligo d(T) priming and cloned into EcoRI and XhoI sites of the Lambda ZAP vector using the ZAP-cDNA Synthesis Kit (Stratagene).

characteristic: description

text: XL1-Blue MRF'

tag: attribute

characteristic: lab-host

text: Toxoplasma gondii

characteristic: organism

text: EcoRI

text: XhoI

text: RH

characteristic: strain

text: TgRHcDNA

characteristic: title

text: phagemid

text: Lambda ZAP

characteristic: vector


text: Instituto de Quimica - Universidade de Sao Paulo

text: live

text: Generic

text: adult

characteristic: dev-stage

text: Mus musculus

text: Schistosoma mansoni

text: mixed pool

characteristic: sex

text: Plasmid

text: pGEM T-easy


text: live

text: Generic

text: cercaria

text: Biomphalaria glabrata

text: Schistosoma mansoni

text: mixed pool

text: Plasmid

text: pGEM T-easy


text: live

text: Generic

text: germball

text: Biomphalaria glabrata

text: Schistosoma mansoni

text: mixed pool

text: Plasmid

text: pGEM T-easy


text: live

text: Generic

text: miracidium

text: Schistosoma mansoni

text: mixed pool

text: Plasmid

text: pGEM T-easy


text: live

text: Generic

text: schistosomulum

text: in vitro culture

text: Schistosoma mansoni

text: mixed pool

text: Plasmid

text: pGEM T-easy


text: live

text: Generic

text: egg

text: Mus musculus

text: Schistosoma mansoni

text: mixed pool

text: Plasmid

text: pGEM T-easy


text: New York University School of Medicine

text: live

text: Generic

text: 10 million Plasmodium yoelii 17X sporozoites were isolated from salivary glands of infected Anospheles stephensi mosquitoes and purified over a DE52 cellulose column. The sporozoites were cultured for 24h at 37C in the presence of 5% CO2 and 10% FBS as described in 'Transformation of sporozoites into early exoerythrocytic malaria parasites does not require host cells ' (J Exp Med. (2003)197(8):1045-50). mRNA was extracted using the Invitrogen microFAST TRACK kit and used for cDNA construction via the BDBiosciences SMART cDNA synthesis kit. Double stranded cDNA was cloned into a plasmid vector using Invitrogen's TOPO TA vector cloning kit.

text: axenic hepatic stages

text: E. coli TOP10

text: Plasmodium yoelii yoelii

text: Plasmodium yoelli 17X axenic hepatic stages

text: Plasmid


text: The University of Georgia, Department of Plant Biology

text: live

text: Generic

text: The library was prepared from polyA+ RNA from drought-stressed loblolly pine (Pinus taeda) roots. Water was withheld from ramet clones until predawn needle water potential reached -1.75 MPa. On day 7 roots were harvested for RNA isolation. Double-stranded cDNA was cloned unidirectionally into pSL1180. Inserts excised with EcoRI (5' end) and XhoI (3' end).

text: DH10B-T1 phage-resistant E. coli

text: Pinus taeda

text: EcoRI

text: XhoI

text: CCLONES

text: Drought-stressed loblolly pine roots DS1


text: live

text: Generic

text: The library was prepared from polyA+ RNA from loblolly pine (Pinus taeda) roots watered to pot capacity every other day. Pre-dawn water potential remained -0.3 MPa +/-0.1. Roots were harvested for RNA isolation. Double-stranded cDNA was cloned unidirectionally into pSL1180. Inserts excised with EcoRI (5' end) and XhoI (3' end).

text: DH10B-T1 phage-resistant E. coli

text: Pinus taeda

text: EcoRI

text: XhoI

text: CCLONES

text: Well-watered loblolly pine roots WW2


text: live

text: Generic

text: DH10B-T1 phage-resistant E. coli

text: Pinus taeda

text: EcoRI

text: XhoI

text: CCLONES

text: Well-watered loblolly pine roots WW3


text: live

text: Generic

characteristic: cultivar

text: The library was prepared from polyA+ RNA from pollen at the late vacuolated-vacuolated stage of development. Pollen was harvested from greenhouse-grown panicles of sorghum line BTx623. Panicles were removed from the flag leaf prior to emergence, when no detectable amylase is present in pollen of male-fertile lines. This stage represents pollen collected from anthers about 8-14 days prior to anthesis. Double-stranded cDNA was cloned unidirectionally into different DraIII sites of the pME18S-FL3 vector (5-prime DraIII site is CACTGTGTG, 3-prime DraIII site is CACCATGTG). XhoI excises the cDNA insert.

text: DH10B-T1 phage-resistant E. coli

text: Pollen

characteristic: organ

text: Sorghum bicolor

text: XhoI

text: XhoI

text: Pollen

text: Plasmid


text: USDA, ARS, U. S. Horticultural Research Laboratory

characteristic: INSDC first public

text: live

text: Generic

text: Valencia

text: Standard library construction protocols from Stratagene cDNA synthesis kit (cat.# 200401-5) and Uni-ZAP XR vector kit (cat.# 237211) were followed using poly(A) RNA.

text: Citrus sinensis

text: EcoRI

text: XhoI

text: Immature ovaries

characteristic: tissue-type

text: Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck)

text: Phagemid

text: pBluescript SK(-)


characteristic: External Id

text: UC Davis, Plant Pathology

characteristic: INSDC center name

text: live

text: Generic

text: Cabernet Sauvignon (Clone 8)

text: CABUD is a cDNA library of Vitis vinifera cv. 'Cabernet Sauvignon' Clone 8 dissected buds. Samples were collected May 13, 2002 from pre-bloom plants (10-11 days before bloom), pre-veraison. Sampled vines were located at the University of California, Davis, Experimental Vineyard. cDNAs were made by oligo-dT priming and directionally cloned. 5'and 3' adaptors were used in cloning as follows: 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' and 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)NN-3'. Library was constructed using the Clontech Creator SMART kit and size-selected to contain the 0.5-3 kb size fraction.

text: Pre-bloom (10-11 days before bloom)

text: Bud

text: Vitis vinifera

text: SfiI

text: SfiI

text: Hermaphrodite

text: Vitis vinifera cv. cabernet sauvignon (Clone 8) Bud - CABUD

text: Plasmid

text: pDNR


text: UC Davis, Plant Pathology

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Cabernet Sauvignon

text: CAP is a cDNA library of Vitis vinifera cv. 'Cabernet Sauvignon' Clone 8 petioles. Samples were collected on July 10, 2002 from plants on the onset of Veraison (berry softening). Sampled vines were located at the University of California, Davis, Experimental Vineyard. cDNAs were made by oligo-dT priming and directionally cloned. 5'and 3' adaptors were used in cloning as follows: 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' and 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)NN-3'. Library was constructed using the Clontech Creator SMART kit and size-selected to contain the 0.5-3 kb size fraction.

text: Onset of Veraison (berry softening)

text: Petiole

text: Vitis vinifera

text: SfiI

text: SfiI

text: Hermaphrodite

text: Vitis vinifera cv. cabernet sauvignon (Clone 8) Petiole - CAP

text: Plasmid

text: pDNR


text: National Botanical Research Institute

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Harichaal

text: Partial cDNA fragments obtained by differential display were cloned in pBSSK+ vector at EcoRV site.

text: Ripened banana

text: fruit

text: Musa acuminata

text: Hermaphrodite

text: Pulp

text: banana fruit

text: Plasmid

text: pBSSK+


text: Hokkaido University

text: live

text: Generic

text: country=Japan

characteristic: description

text: erythrocyte stage

text: Theileria orientalis

text: Theileria orientalis lambda TriplEx2 cDNA library, erythrocyte stage


text: National Institute of Agrobiological Sciences

text: live

text: Generic

text: neonatal, 5 weeks

text: Sus scrofa

characteristic: organism

text: male

text: hippocampus

text: full-length enriched pig cDNA library, neonatal, 5 weeks, hippocampus


text: University of Puerto Rico. Department of Biology

text: live

text: Generic

text: cDNA was synthesized from 2 ug of total RNA from intestine using random primers and the ThermoScript RT-PCR system (Invitrogen). The EST was amplified and RT-PCR cloned using a set of primers designed from the sequence of the Holothuria glaberrima (EpenHg-AY383544) ependymin-related gene: EpenHgF1:5-CGGGGATCAATGTTTCACC-3 and EpenHgR:5-GGAGTGTAGATGCCAATCCAGC-3

text: Adult

text: TOP10 (Invitrogen)

characteristic: lab-host

text: Intestine

text: Holothuria mexicana

text: EpenHm ependymin-related EST RT-PCR cloned

text: Plasmid

text: pCR4-TOPO TA cloning vector (Invitrogen)


text: live

text: Generic

text: cDNA was synthesized from 2 ug of total RNA from intestine using random primers and the ThermoScript RT-PCR system (Invitrogen). The EST was amplified and RT-PCR cloned using a set of primers designed from the sequence of the Holothuria glaberrima (EpenHg-AY383544) ependymin-related gene: EpenHgF1:5-CGGGGATCAATGTTTCACC-3 and qEpenHgR:5-CAGCAACCACCATTCTCTGTT-3

text: Adult

text: TOP10 (Invitrogen)

text: Intestine

text: Lytechinus variegatus

text: EpenLv ependymin-related EST RT-PCR cloned

characteristic: title

text: Plasmid


text: Nippon Shinyaku Co. Ltd.

text: live

text: Generic

text: leukemia cell

characteristic: cell type

text: mRNA expressed in leukemia cells (mixture of CCRF-CEM, K562, HL-60, MOLT-4, or RPMI-8226)

text: Homo sapiens

text: leukemia cell normalized cDNA library


text: Nippon Shinyaku Co. Ltd.

text: live

text: Generic

text: leukemia cell

text: mRNA upregulated in Ara-C-treated leukemia cells (mixture of CCRF-CEM, K562, HL-60, MOLT-4, or RPMI-8226)

text: Homo sapiens

text: leukemia cell subtracted cDNA library


text: Justus-Liebig University Giessen

text: live

text: Generic

text: Ingrid

text: 7 d after germination

characteristic: dev-stage

text: Hordeum vulgare subsp. vulgare

text: Hordeum vulgare subsp. vulgare 7 d after germination


text: Temple University School of Medicine

text: live

text: Generic

text: isolated by kinetic enrichment in a representational difference analysis (RDA) experiment

text: fetus

text: Homo sapiens

text: inner and middle ear

text: Homo sapiens fetus inner and middle ear


text: University of Regensburg

text: live

text: Generic

characteristic: cell-line

text: selected for increased malignancy potential

text: Homo sapiens

text: melanoma

text: Homo sapiens AP32


text: University of Regensburg

text: live

text: Generic

text: Homo sapiens

text: melanoma

text: Homo sapiens A375P


text: University of Regensburg

text: live

text: Generic

text: Homo sapiens

text: melanoma

text: Homo sapiens A375C


text: University of Regensburg

text: live

text: Generic

text: SI

text: Homo sapiens

text: melanoma

text: Homo sapiens SI


text: University of Regensburg

text: live

text: Generic

text: Homo sapiens

text: melanoma

text: Homo sapiens AP30


text: University of Regensburg

text: live

text: Generic

text: Homo sapiens

text: melanoma

text: Homo sapiens A375SM


text: University of Regensburg

text: live

text: Generic

text: Homo sapiens

text: melanoma

text: Homo sapiens A375M


text: Chinese PLA General Hospital

text: live

text: Generic

text: taxol induced; drug resistant

text: Homo sapiens

text: cancerous ovary

text: Homo sapiens SKOV3/TAXOL


text: Research Center Borstel

text: live

text: Generic

text: adult

text: Homo sapiens

text: lung adenocarcinoma

text: Homo sapiens LT23


text: National Institutes of Health

text: live

text: Generic

text: Homo sapiens

text: Homo sapiens library (Gu J)


text: PUMC

text: live

text: Generic

text: 2 month-old adult

text: Rattus norvegicus

text: Wistar

characteristic: strain

text: testis

text: Rattus norvegicus testis Wistar 2 month-old adult


text: Instituto de Investigaciones Biotecnologicas

text: live

text: Generic

text: mRNA from the hippocampal formation of a pool of cortisol-treated animals was subtracted with excess mRNA obtained from the hippocampal formation of a pool of control animals. The library was constructed using the PCR-Select cDNA Subtraction Kit (Clontech, Palo Alto, CA).

text: adult

text: brain

characteristic: organ

text: Tupaia belangeri

text: hippocampus

text: tu-t-c

text: Plasmid

text: pGEM-T Easy

characteristic: vector


characteristic: INSDC first public

text: live

text: Generic

text: mRNA from the hippocampal formation of a pool of control animals was subtracted with excess mRNA obtained from the hippocampal formation of a pool of cortisol-treated animals. The library was constructed using the PCR-Select cDNA Subtraction Kit (Clontech, Palo Alto, CA).

text: adult

text: brain

text: Tupaia belangeri

text: hippocampus

characteristic: tissue-type

text: tu-c-t

text: Plasmid

text: pGEM-T Easy


characteristic: External Id

text: Harbin Medical University

characteristic: INSDC center name

text: live

text: Generic

text: AGZY83-a; Anip973

text: Homo sapiens

text: lung adenocarcinoma

text: Homo sapiens AGZY83-a, Anip973


text: Horticulture Research International

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Rebecca

characteristic: cultivar

text: A suppressive subtraction using a Clontech SSH kit was carried out using RNA from Stage 0 petals (closed bud) as the tester and Stage 2 petals (just open flower) as the driver. The resulting library should be enriched for genes expressed at stage 0.

text: expanded bud

text: flower

text: Alstroemeria peruviana

text: petal

text: Alstroemeria petal S0-S2 subtracted

text: plasmid

text: pGEMT (Promega)


text: Virginia Tech

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Meleagris gallopavo

text: pituitary

text: Meleagris gallopavo pituitary


text: Academic Medical Center

text: live

text: Generic

text: adult

text: Homo sapiens

characteristic: organism

text: brain

text: Homo sapiens brain adult


text: USDA-ARS and University of Wisconsin

text: live

text: Generic

text: Addis

text: Description of library preparation methods, cDNA library described in Theor Appl Genet. 89:42-48 (1994).

characteristic: description

text: Cucumis sativus

text: EcoRI

text: EcoRI

text: cDNA library of cucumber cultivar Addis

characteristic: title

text: plasmid


text: EBC, Uppsala University

text: live

text: Generic

text: hemocytes

characteristic: cell type

text: Pacifastacus leniusculus

text: male

characteristic: sex

text: Crayfish Lambda Zap Express Library

text: Zap Express


text: National Cancer Institute / NIH

text: live

text: Generic

text: DH10B TonA

characteristic: lab-host

text: brain/CNS

text: Danio rerio

text: DraIII

text: DraIII

text: phagemid (ampicillin resistant)


text: National Cancer Institute / NIH

text: live

text: Generic

text: DH10B TonA

text: liver

characteristic: organ

text: Danio rerio

text: DraIII

text: DraIII

text: phagemid (ampicillin resistant)


text: National Cancer Institute / NIH

text: live

text: Generic

text: RNA obtained from three placentas from female C57/BL6 mouse at 16 days pregnancy. Tissues were snap-frozen and kept at -80C for two days before RNA extraction and purification (Tri-reagent method). cDNA was primed using oligo-dT primer: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3' and cloned into the EcoRV/NotI sites of pExpress-1. Size-selection >1kb resulted in an average insert size of 1.3 kb. This primary, microquantity library is normalized to Cot5 (non-normalized primary library is NIH_MGC_222) and was constructed by Express Genomics (Frederick, MD).

text: DH10B (phage-resistant)

text: placenta

text: Mus musculus

text: EcoRV

text: NotI

text: plasmid (ampicillin resistant)

characteristic: vector


text: National Cancer Institute / NIH

characteristic: INSDC first public

text: live

text: Generic

text: RNA obtained from three placentas from female C57/BL6 mouse at 16 days pregnancy. Tissues were snap-frozen and kept at -80C for two days before RNA extraction and purification (Tri-reagent method). cDNA was primed using oligo-dT primer: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3' and cloned into the EcoRV/NotI sites of pExpress-1. Size-selection >0.75kb resulted in an average insert size of 1.1 kb. This primary, nanoquantity library is normalized to Cot5 (non-normalized primary library is NIH_MGC_223) and was constructed by Express Genomics (Frederick, MD).

text: DH10B (phage-resistant)

text: placenta

text: Mus musculus

text: EcoRV

text: NotI

text: plasmid (ampicillin resistant)


text: National Cancer Institute / NIH

text: live

text: Generic

text: RNA obtained from three placentas from female C57/BL6 mouse at 16 days pregnancy. Tissues were snap-frozen and kept at -80C for two days before RNA extraction and purification (Tri-reagent method). cDNA was primed using oligo-dT primer: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3' and cloned into the EcoRV/NotI sites of pExpress-1. Size-selection >1 kb resulted in an average insert size of 1.5 kb. Library is not amplified. (Normalized version of this library is NIH_MGC_203.) Library constructed by Express Genomics (Frederick, MD).

text: DH10B (phage-resistant)

text: placenta

text: Mus musculus

text: EcoRV

text: NotI

text: plasmid (ampicillin resistant)


text: Royal Vet. and Agricultural University

text: live

text: Generic

text: adult

characteristic: dev-stage

text: Sus scrofa

text: small intestine

characteristic: tissue-type

text: LambdaZapII, pig adult small intestine


characteristic: External Id

text: Max-Planck-Institut fuer molekulare Genetik

characteristic: INSDC center name

text: live

text: Generic

text: oligo-dT-NotI primed, SalI adaptors, directionally cloned, library normalised by oligonucleotide fingerprinting (OFP)

text: late somitogenesis

text: Escherichia coli, XL1 blue

text: Danio rerio

text: NotI

text: SalI

text: wild type

characteristic: strain

text: whole embryo

text: zebrafish late somitogenesis cDNA library ICRFp524

text: Plasmid


characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: oligo-dT-NotI primed, SalI adaptors, directionally cloned, library normalised by oligonucleotide fingerprinting

text: shield stage (gastrula)

text: Escherichia coli, XL1 blue

text: Danio rerio

text: NotI

text: SalI

text: wild type

text: whole embryo

text: zebrafish shield stage embryo (gastrula) MPMGp567

text: Plasmid


text: Princeton University

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Nymphs and adults colonies

text: Acyrthosiphon pisum

text: whole body

text: Pea aphid, whole body


text: Institut de Biotechnologie des Plantes ; John Innes Centre

text: live

text: Generic

text: T.aestivum riband

characteristic: cultivar

text: Seeds of wheat were surface-sterilized, germinated and grown in vermiculite mixed with 7 days old mycelium of Gaeumannomyces graminis. Infected wheat roots were collected after 4 days of infection, frozen and RNA extraction was performed (Vallelian-Bindschedler et al. 1998). cDNA was synthesised from infected, non-infected tissue and the fungus growing in a complete medium using SMART cDNA Synthesis Kit (Clontech). The subtracted library was constructed using the PCR-select (Clontech).

characteristic: description

text: 4 days after inoculation

text: E.coli strain DH10B

text: Triticum aestivum/Gaeumannomyces graminis mixed EST library

characteristic: organism

text: Infected Roots

text: Subtracted library from Infected wheat roots with G.graminis

text: plasmid

text: pCR2.1 T/A vector (Invitrogen)


text: live

text: Generic

text: T.aestivum riband

text: Seeds of wheat were surface-sterilized, germinated and grown in vermiculite mixed with 7 days old mycelium of Gaeumannomyces graminis. Infected wheat roots were collected after 4 days of infection, frozen and RNA extraction was performed (Vallelian-Bindschedler et al. 1998). cDNA was synthesised using the ZAP Express cDNA Synthesis Kit and a lambda cDNA library constructed using the ZAP Express cDNA Gigapack III Gold Cloning kit (Stratagene). Plasmids were excised from the vector and recovered in the E. coli strain XLOLR.

text: 4 days after inoculation

text: XLOLR

text: Infected Roots

text: Infected wheat roots with G.graminis Lambda Zap Express Library

characteristic: title

text: excised phagemid

text: pBK-CMV,Stratagene


text: Duke University

text: live

text: Generic

text: Stress condition III library, constructed by Jeanette Quinn and Chiung-Wen Chang, combines cDNAs from CC-1690 cells grown to mid-log phase in copper-free TAP medium (see Quinn and Merchant(1998) Methods in Enzymology, 2997:263-279)in a shaking(250 rpm) illuminated (about 100 micromole/m2/sec) incubator at 22 C (see Quinn and Merchant(1998) Methods in Enzymology, 2997:263-279); CC-1690 cells grown to mid-log phase in low Fe (1 micromolar Fe) TAP medium (see La Fontaine S, Quinn JM, Nakamoto SS, Page MD, Gohre V, Moseley JL, Kropat J, Merchant S.LaFontaine et al. (2002) Eukaryotic Cell, 1:736-757) in a shaking illuminated incubator (same conditions as above). CC-1690 cells were grown to mid-log phase in TAP medium in a shaking illuminated incubator to a density of 8x10e6 cells/ml. The flask was transferred to a shaking platform (200 rpm) at room temperature (23C) 12 micromole/m2/sec illumination and bubbled in a stoppered flask with 98% nitrogen, 2% CO2 gas mixture for 1 hour prior to harvesting for RNA isolation (as per Quinn JM, Barraco P, Eriksson M, Merchant S. Quinn et al. (2000) JBC 275:6080-6089); CC-1690 cells grown to mid-log phase (3x10e6 cells/ml) in TAP medium in a shaking (150 rpm) illuminated (70 mole photon/m2/sec) incubator at 27 C. Cells were diluted to 1x10e6 cells/ml, transferred to high light (11000 mol photon/m2/s) with shaking (150 rpm) and sampled at (0.5, 1,2,4,6, 12 hrs); CC-1690 cells grown to mid-log phase in HS medium in a shaking (150 rpm) illuminated (70 mole photon/m2/sec) incubator at 27 C. Cells were diluted to 1x10e6 cells/ml, transferred to high light (11000 mol photon/m2/s) with shaking (150 rpm) and sampled at (0.5, 1,2,4,6, 12 hrs). PolyA mRNA was purified from each sample, pooled and cDNA synthesized (see Shrager et al, Plant Physiol. 131, 401-408 for details). The cDNA was directionally cloned into lambda Zap II (Stratagene) in the EcoRI (5') and XhoRI (3') sites. pBluescript II SK- plasmids were excised from the lambda ZAP clones by superinfection with ExAssist (Stratagene) phage. The library was normalized using method 4 described in Bonaldo et al., (1996) Genome Research 6: 791-806.

text: Chlamydomonas reinhardtii

text: EcoRI

text: XhoI

text: CC-1690 wild type mt+ 21gr

text: C. reinhardtii CC-1690 (mt+), Stress III (normalized), Lambda Zap II

text: plasmid

text: pBluescript II SK-


text: FAMU, USDA-ARS;

text: live

text: Generic

text: A high quality EST with at least 100 contiguous bases at Trace Tuner score of 20 or better. Construction by PM Dang, USDA, ARS, U.S. Horticultural Research Lab, Ft.Pierce, FL, USA.

text: At blooming

characteristic: lab-host

text: Vitis shuttleworthii

text: EcoRI

text: XhoI

text: Mixed population

characteristic: sex

text: Entire tendril, leaves, bud, flowers

text: Vitis shuttleworthii L., grape

text: pBluescript II SK+


text: Department of Physiology, Pharmacology and Neurology, State University of New York Downstate Medical Center

text: live

text: Generic

text: Oligocapping with 5'RACE using GeneRacer kit (Invitrogen)

text: Three month old

text: Brain

characteristic: organ

text: Rattus norvegicus

text: male

text: Sprague-Dawley

text: Hippocampus

text: 5'UTR of PKMzeta (PKCzetaII)


text: Kansas State University

characteristic: INSDC first public

text: live

text: Generic

text: EST from wheat (Triticum aestivum, cv. OK91G106) root in response to aluminum stress

text: Triticum aestivum

text: root

text: Subtracted, Clontech (cat. # K1804-1)


text: Horticulture Research International

text: live

text: Generic

text: Rebecca

text: A suppressive subtraction using a Clontech SSH kit was carried out using RNA from Stage 2 petals (just open flower) as the tester and Stage 4 and 5 petals (early senescing flower) as the driver. The resulting library should be enriched for genes expressed at stage 2.

text: expanded flower

characteristic: dev-stage

text: flower

text: Alstroemeria peruviana

text: petal

text: Alstroemeria petal S2-S4/5 subtracted

text: plasmid

text: pGEMT (Promega)

characteristic: vector


characteristic: External Id

text: Horticulture Research International

text: live

text: Generic

text: Rebecca

text: A suppressive subtraction using a Clontech SSH kit was carried out using RNA from Stages 4 and 5 mixed petals (early senescing flower) as the tester and Stage 2 petals (just open flower) as the driver. The resulting library should be enriched for genes expressed at stages 4 and 5.

text: expanded flower

text: flower

text: Alstroemeria peruviana

text: petal

characteristic: tissue-type

text: Alstroemeria petal S4/5-S2 subtracted

text: plasmid

text: pGEMT (Promega)


text: Horticulture Research International

characteristic: INSDC center name

text: live

text: Generic

text: Rebecca

text: A suppressive subtraction using a Clontech SSH kit was carried out using RNA from Stage 2 petals (just open flower) as the tester and Stage 0 petals (closed bud) as the driver. The resulting library should be enriched for genes expressed at stage 2.

text: expanded flower

text: flower

text: Alstroemeria peruviana

text: petal

text: Alstroemeria petal S2-S0 subtracted

text: plasmid

text: pGEMT (Promega)


text: SUNY College of Environmental Science & Forestry

characteristic: INSDC last update

text: live

text: Generic

text: 2 years+

text: Castanea dentata

text: stem

text: American Chestnut cDNA stem library


text: Hawaii Agriculture Research Center

text: live

characteristic: INSDC status

text: Generic

text: Gene-specific primer (degenerate) to GHYTQVW conserved domain of PR1 protei ns

text: 3 month old seedlings

text: Carica papaya

text: kapoho

characteristic: strain

text: leaves

text: Papaya 3' RACE in PCR-Script


text: Washington University School of Medicine

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: The library was constructed by Claire Murphy and Dr. Makedonka Dautova at Washington University, St. Louis. Oligo(dT)-SL1 PCR based library. Heterodera schachtii J2 cDNA PCR products of size >400 nucleotides containing SL1 on the 5' end and oligo(dT) on the 3' end were non-directionally cloned into pCRII-TOPO(Invitrogen) following the Topo TA cloning protocol. Heterodera schachtii J2 were provided by Dr.Bartel Vanholme of Ghent University (Bartel.Vanholme@rug.ac.be).

text: Heterodera schachtii

text: EcoRI

text: EcoRI

text: Heterodera schachtii J2 SL1 TOPO v2

text: plasmid (kanamycin and ampicillin resistant)

text: pCRII-TOPO (Invitrogen)


text: USDA-ARS

text: live

text: Generic

text: Acyrthosiphon pisum colonies. Library by Srini Kambhampati; Peter Dearden; David Stern, Department of Ecology and Evolutionary Biology, Princeton University, NJ. (609) 258-0759. A high quality EST with at least 100 contiguous bases at Trace Tuner score of 20 or better. Sequencing of clones by Dr. PM Dang, US. Horticultural Research Lab, Ft. Pierce, FL. (772) 462- 5940.

characteristic: description

text: All

text: Acyrthosiphon pisum

text: EcoRI

text: XhoI

text: Mixed population

text: whole body, nymphs and adults

text: Acyrthosiphon pisum, Pea Aphid

text: pBluescript II SK+


text: National Cancer Institute / NIH

text: live

text: Generic

text: Library is oligo-dT primed and directionally cloned. 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.30 kb (range 0.60-3.5 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA).

text: DH10B (T1 phage-resistant)

text: Homo sapiens

characteristic: organism

text: SfiI (ggccattatggcc)

text: SfiI (ggccgcctcggcc)

text: Stomach, adenocarcinoma

text: plasmid (ampicillin-resistant)

text: pDNR-LIB


text: National Cancer Institute / NIH

text: live

text: Generic

text: Library is oligo-dT primed and directionally cloned. Granulosa lutein cells aspirated from preovulatory folicles of normal cycling women undergoing ovulation induction for infertility due to male factor and normal doners. The cells were from follicles stimulated with Lupron, FSH and hCG. 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 2.23 kb (range 1.0-4.5 kb). 14/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA).

text: DH10B (T1 phage-resistant)

text: Homo sapiens

text: SfiI (ggccattatggcc)

text: SfiI (ggccgcctcggcc)

text: Ovary

characteristic: title

text: plasmid (ampicillin-resistant)

text: pDNR-LIB


text: National Cancer Institute / NIH

text: live

text: Generic

text: Library is oligo-dT primed and directionally cloned Denatured RNA was size fractionated on a 1% agarose gel. First strand cDNA synthesis was primed with oligo-dT primer containing a Not I site. Double strand cDNA was size selected according tomRNA size fraction, ligated with EcoR I adaptor, digested with Not I and then cloned directionally into pYX-Asc vector. Average insert size 0.5-1Kb. Adaptors 5'(AATTCGGCACGAGG)3' and 5'd (CCTCGTGCCG)3'. 3' Linker sequence - GCGGCCGCTGAGAGCC T18. Sequencing primers 3'end: T3 promoter primer 5'd (ATTAACCCTCACTAAAGGGA)3'. 5' End: T7 promoter primer 5'd (TAATACGACTCACTATAGGG)3'. Library was constructed in the laboratory of M. Bento Soares. Average insert size 1-2kb Note: this is a NIH_MGC Library.

text: DH10B (T1 phage-resistant)

characteristic: lab-host

text: Homo sapiens

text: EcoRI

text: NotI

text: Pooled Chondrosarcoma Tumor cells

text: plasmid (ampicillin-resistant)

text: pYX-Asc


text: National Cancer Institute / NIH

text: live

text: Generic

text: Library is oligo-dT primed and directionally cloned Denatured RNA was size fractionated on a 1% agarose gel. First strand cDNA synthesis was primed with oligo-dT primer containing a Not I site. Double strand cDNA was size selected according tomRNA size fraction, ligated with EcoR I adaptor, digested with Not I and then cloned directionally into pYX-Asc vector. Average insert size 0.5-1Kb. Adaptors 5'(AATTCGGCACGAGG)3' and 5'd (CCTCGTGCCG)3'. 3' Linker sequence - GCGGCCGCTGAGAGCC T18. Sequencing primers 3'end: T3 promoter primer 5'd (ATTAACCCTCACTAAAGGGA)3'. 5' End: T7 promoter primer 5'd (TAATACGACTCACTATAGGG)3'. Average insert size 0.5-1kb. Library was constructed in the laboratory of M. Bento Soares. Note: this is a NIH_MGC Library.

text: DH10B (T1 phage-resistant)

text: Homo sapiens

text: EcoRI

text: NotI

text: Pooled Chondrosarcoma Tumor cells

text: plasmid (ampicillin-resistant)

text: pYX-Asc


text: National Cancer Institute / NIH

text: live

text: Generic

text: Library is oligo-dT primed and directionally cloned Denatured RNA was size fractionated on a 1% agarose gel. First strand cDNA synthesis was primed with oligo-dT primer containing a Not I site. Double strand cDNA was size selected according tomRNA size fraction, ligated with EcoR I adaptor, digested with Not I and then cloned directionally into pYX-Asc vector. Average insert size 0.5-1Kb. Adaptors 5'(AATTCGGCACGAGG)3' and 5'd (CCTCGTGCCG)3'. 3' Linker sequence - GCGGCCGCTGAGAGCC T18. Sequencing primers 3'end: T3 promoter primer 5'd (ATTAACCCTCACTAAAGGGA)3'. 5' End: T7 promoter primer 5'd (TAATACGACTCACTATAGGG)3'. Library was constructed in the laboratory of M. Bento Soares. Average insert size 2-3kb. Note: this is a NIH_MGC Library.

text: DH10B (T1 phage-resistant)

text: Homo sapiens

text: EcoRI

text: NotI

text: Pooled Chondrosarcoma Tumor cells

text: plasmid (ampicillin-resistant)

text: pYX-Asc


text: IRD

text: live

text: Generic

characteristic: cultivar

text: Clontech PCR-select cDNA subtraction library ; Hypersensitive reaction (HR)-specific (incompatible interaction as tester and compatible interaction as driver in the subtraction process); leaves were collected 12 h post-inoculation with Hemileia vastatrix

text: E.coli

text: leaf

text: Coffea arabica

text: Coffee SSH library 1

text: Plasmid

text: pGEM-T easy


text: IRD

text: live

text: Generic

text: Clontech PCR-select cDNA subtraction library ; Hypersensitive reaction (HR)-specific (incompatible interaction as tester and compatible interaction as driver in the subtraction process); leaves were collected 24 and 48 h post-inoculation with Hemileia vastatrix and mRNAs were pooled in equal amount

text: E.coli

text: leaf

characteristic: organ

text: Coffea arabica

text: Coffee SSH library 2

text: Plasmid

text: pCRII-TOPO


text: Istituto Agrario di San Michele all'Adige (IASMA)

characteristic: INSDC first public

text: live

text: Generic

text: Pinot noir

text: veraison

text: berry

text: Vitis vinifera

text: NotI

text: SalI

text: Hermaphrodite

characteristic: sex

text: Grape Berry pSPORT1 Library

text: plasmid


text: live

text: Generic

text: Pinot noir

text: young root (first year)

characteristic: dev-stage

text: root

text: Vitis vinifera

text: NotI

text: SalI

text: Hermaphrodite

text: Grape Root pSPORT1 Library

text: plasmid

characteristic: vector


characteristic: External Id

text: live

text: Generic

text: Pinot noir

text: juvenile

text: XL1-Blue MRF'

text: leaf

text: Vitis vinifera

text: EcoRI

text: XhoI

text: Hermaphrodite

text: Grape Leaf pBluescript Library

text: Lambda Uni-Zap XR, pBluescript SK-


characteristic: INSDC center name

text: live

text: Generic

text: Pinot noir

text: bud swelling

text: bud

text: Vitis vinifera

text: NotI

text: SalI

text: Hermaphrodite

text: Grape Bud pSPORT1 Library

text: plasmid


characteristic: INSDC last update

text: live

text: Generic

text: Regent

text: growing shoot tip (ca. 1 cm long)

text: shoot tip

text: Vitis vinifera

text: SalI

text: NotI

text: Hermaphrodite

text: Grape Shoot pSPORT1 Library

text: plasmid


text: Cardiff University

text: live

characteristic: INSDC status

text: Generic

text: The library was prepared using protocols given by the supplier (Stratagene).

text: Adult

text: Lumbricus rubellus

text: Whole worm

characteristic: tissue-type

text: Earthworm Cadmium Exposure Library

text: pBluescript II SK+


text: CSIRO Livestock Industries

text: live

text: Generic

characteristic: NCBI submission model

text: Library made from subcutaneous fat of a 14 month old Angus steer.

text: Young Adult

text: Bos taurus

text: EcoRI

text: Xho I

text: male

text: Angus

characteristic: strain

text: subcutaneous fat tissue

text: Bos taurus fat cDNA library

text: Uni-ZAPXR


text: New Mexico State University

text: live

text: Generic

characteristic: NCBI submission package

text: Rhizophagus intraradices

text: G. intraradices extraradical mycelium


text: University of Illinois, Urbana-Champaign

text: live

text: Generic

text: Samples were collected in Robert E. Sharp's lab (University of Missouri-Columbia) to construct three normalized cDNA libraries. Dark-grown maize seedlings with primary roots 12-20 mm in length were transplanted to high (-0.03 MPa) or low water potential (-1.6 MPa) vermiculite, and harvested at 5 h and 48 h after transplanting. About 1,000 roots were used for each of the low water potential libraries (zmrws05 and zmrws48) while 500 roots were combined from each of the two time points at high water potential (zmrww00). Each root was divided into 4 segments (distances are from the junction of the root apex and root cap): segment 1, 0-3 mm plus the root cap; segment 2, 3-7 mm; segment 3, 7-12 mm; segment 4, 12-20 mm. (For detatils of conditions see (1) with nutrient modifications as in (2)). The three normalized cDNA libraries were constructed in the lab of Hans Bohnert (University of Illinois-UC). Total RNA was extracted by the 'hot Phenol' method (Plant Molecular Biology manual. D5: 1-13, 2nd ed., 1997). This method worked in eliminating carbohydrate material present in the root tips. The integrity of the RNA was verified by denaturing agarose gels and spectrophotometry (ratio A260/280). Poly(A)+mRNA was isolated twice from total RNA using the Oligotex Direct mRNA kit (Qiagen). Poly(A)+ mRNA was converted to double-stranded cDNA and tagged by using modified Oligo(dT) primers. One of 4 sequence tags corresponding to a different segment of the root was added to the 3'-end of the modified Oligo(dT) primers, including a Not1 site and used to reverse transcribe the segment-specific mRNAs into cDNAs. Each library contains all four tags. A suffix (s1, s2, s3, or s4) has been added to each sequence identifier to designate which region of the root (Root_segment_1, 2, 3, or 4) the sequence was found in based on the identification of the tag. A suffix of s0 indicates that the sequence tag, and hence the source segment, could not be identified. The double stranded cDNAs were size-selected (>450 bp). Size selected cDNAs were adaptored with EcoRI adaptors at both ends, and then digested with NotI. The cDNA was directionally cloned into EcoR1-NotI digested pBS II SK(+) phagemid vector (Stratagene) and electroporated into E.coli DH10B. The total number of white colony forming units (cfu) in the primary libraries before amplification was as follows: zmrws05: 3.37 x 106; zmrws48: 4.87 x 106; zmrww00: 3 x 106. The background of empty clones was less than 1%. Inserts ranged from ~0.5kb to >2.5 kb, as determined by PCR. Plasmid DNA from the primary libraries then was converted to single-stranded circles and used as a template for PCR amplification using the T7 and T3 priming sites that flank the cloned cDNA inserts. The purified PCR products, representing the entire cDNA population cloned in each library, were used as a driver for normalization. Hybridization between the single-stranded library and the PCR products was carried out for 44 hours at 30oC. Non-hybridized single-stranded DNA circles were separated from hybridized DNA rendered partially double-stranded and electroporated into DH10B. The total number of clones with insert was: zmrws05: 2.0x107; zmrws48: 4.2x107; zmrww00: 1.1x107. The background of empty clones was less than 2%. Insert size, determined by PCR of the entire library, ranged from 0.5kb to 2.5kb. (1) Sharp R E; Silk W K; Hsiao T C. Growth of the Maize Primary Root at Low Water Potentials I. Spatial Distribution of Expansive Growth. Plant Physiology (Rockville). 87(1). 1988. 50-57. (2) Spollen W G; LeNoble M E; Samuels T D; Bernstein N; Sharp R E. Abscisic acid accumulation maintains maize primary root elongation at low water potentials by restricting ethylene production. Plant Physiology (Rockville). 122(3). March, 2000. 967-976.

characteristic: description

text: Zea mays

characteristic: organism


text: Bielefeld University

text: live

text: Generic

text: Wassilewskija-0 (Ws-0)

text: cDNA library from Arabidopsis thaliana, accession Wassilewskija-0; roots from three weeks old plants grown on MS-plates at 26M-0C with 16 hours light/day; library was made at the Max-Planck-Institute for Plant Breeding Research, Cologne, Germany; cloning sites SalI-NotI, primer sites and orientation: SP6-SalI-CCACGCGTCCG-5prime-cDNA-polyA-CC-NotI-T7; GATEWAY compatible; Note: Sequencing granted in the context of the GABI Arabidopsis Verbund I: Genetic Diversity, 'Establishment of high-efficiency SNP-based mapping tools and development of methods for genome-wide mutation detection' PI: Bernd Weisshaar Sequence submission managed by RZPD/GABI-Primary database: http://gabi.rzpd.de This clone is available from RZPD; contact RZPD (clone@rzpd.de) for further information.

text: E. coli TOP10

characteristic: lab-host

text: Arabidopsis thaliana

text: SalI

text: root

characteristic: title


text: Technical University of Vienna

text: live

text: Generic

text: sequence tags differentially expressed between Hypocrea jecorina wildtype strain QM9414 and cellulase non inducible strain QM9978 under inducing conditions (sophorose)

text: Trichoderma reesei

text: Differential expression data from subtraction hybridization experiment


text: Institute of Hydrobiology, the Chinese Academy of Sciences

text: live

text: Generic

text: Tetrahymena thermophila

text: Suppression Subtractive Hybridization Libraries of Tetrahymena thermophila Exposed in Dichlorodiphenyltrichloroethane (DDT)


text: Faculty of Agriculture and Natural Science, University of Pretoria

text: live

text: Generic

text: anamorph: Fusarium circinatum; mRNA/cDNA

characteristic: isolate

text: Fusarium circinatum

text: Transcript derived fragments from Fusarium circinatum using SSH


text: The University of Georgia, Department of Plant Biology

characteristic: INSDC first public

text: live

text: Generic

text: The library was prepared from polyA+ RNA from the roots of 1-year-old loblolly pine (Pinus taeda) cuttings that were rooted and then planted in washed sand. Just before harvesting roots for RNA isolation, the rooted cuttings were maintained for 27 days (April 2003) under ambient conditions in a local greenhouse. They were kept on a weekly regimen of 0.5x nutrient-complete Hoagland's solution and supplemented with additional water sufficient to maintain a 15% soil moisture content. Double-stranded cDNA was cloned unidirectionally into pSL1180. Inserts can be excised with EcoRI (5' end) and XhoI (3' end).

text: DH10B-T1 phage-resistant E. coli

text: root

characteristic: organ

text: Pinus taeda

text: EcoRI

text: XhoI

text: 3 CCLONES

text: Root control


text: Childrens Hospital of Philadelphia

text: live

text: Generic

text: Homo sapiens

text: Sclera


text: IB-Universidade Estadual de Campinas

text: live

text: Generic

text: The Melipona quadrifasciata cDNA fragments were obtained by DDRT-PCR technique. Total RNA was prepared from whole body of newly emerged queens and workers of Melipona quadrifasciata stingless bee.

text: newly emerged

characteristic: dev-stage

text: DH10B E.coli

text: whole body

text: Melipona quadrifasciata

text: EcoRI

text: SpeI

text: female

characteristic: sex

text: Melipona quadrifasciata cDNA fragments

text: pGEM-T Easy vector


characteristic: External Id

text: Institut de Recerca i Tecnologia Agroalimentaries

text: live

text: Generic

text: PI 161375

characteristic: cultivar

text: Cucumis melo

text: Young plant

text: Melon Lambda Zap Express Library


text: Texas A&M University

characteristic: INSDC center name

characteristic: INSDC last update

text: live

text: Generic

text: ATx399 X RTx430

text: The subtractive cDNA library, consisting of both forward and reverse subtractions, was prepared from polyA+ RNA from sorghum seedlings infested as well as uninfested by greenbug aphids, respectively. The Clontech PCR-select cDNA subtraction kit protocol was followed in library construction.

text: one-week old seedlings

text: E. coli

text: Sorghum bicolor

text: leaf

text: Subtractive cDNA library from sorghum infested by greenbug aphids

text: Plasmid

text: pT-Adv

characteristic: vector


text: South China Agricultural University

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Volvariella volvacea

text: mycelium

characteristic: tissue-type

text: Cold stress Volvariella volvacea mycelium cDNA library


text: live

text: Generic

characteristic: NCBI submission package

text: The library was constructed according Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. Denatured RNA was size fractionated on a 1% agarose gel.First strand cDNA synthesis was primed with oligo-dT primer containing a Not I site. Double strand cDNA was size selected according to mRNA size fraction, ligated with EcoR I adaptor, digested with NotI and then cloned directionally into pYX-Asc vector. The library tag sequence located between the Not I site and the polyA tail is TTATTGAAGT. This library was created for the University Iowa Brain Anatomy Project (BMAP): 'Gene Discovery in the Developing Mouse Nervous System', supported by National Institute of Mental Health (NIMH), Hemin Chin, Ph.D., program coordinator.

characteristic: description

text: embryo 12.5,13.5,14.5 dpc

text: DH10B (T1 phage resistant)

text: Eye

text: Mus musculus

text: EcoR I

text: Not I

characteristic: strain

text: whole eye

text: Plasmid

text: pYX- Asc


text: live

text: Generic

text: The library was constructed according Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. Denatured RNA was size fractionated on a 1% agarose gel.First strand cDNA synthesis was primed with oligo-dT primer containing a Not I site .Double strand cDNA was size selected according to mRNA size fraction ,ligated with EcoR I adaptor , digested with NotI and then cloned directionally into pYX-Asc vector . The library tag sequence located between the Not I site and the polyA tail is TTATTGAAGT. This library was created for the University Iowa Brain Anatomy Project (BMAP): 'Gene Discovery in the Developing Mouse Nervous System', supported by National Institute of Mental Health (NIMH), Hemin Chin , Ph.D. , program coordinator.

text: embryo 12.5,13.5,14.5 dpc

text: DH10B (T1 phage resistant)

characteristic: lab-host

text: Eye

text: Mus musculus

characteristic: organism

text: EcoR I

text: Not I

text: whole eye

characteristic: title

text: Plasmid

text: pYX- Asc


characteristic: INSDC first public

text: live

text: Generic

text: The library was constructed according Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. Denatured RNA was size fractionated on a 1% agarose gel.First strand cDNA synthesis was primed with oligo-dT primer containing a Not I site .Double strand cDNA was size selected according to mRNA size fraction ,ligated with EcoR I adaptor , digested with NotI and then cloned directionally into pYX-Asc vector . The library tag sequence located between the Not I site and the polyA tail is TTATTGAAGT. This library was created for the University Iowa Brain Anatomy Project (BMAP): 'Gene Discovery in the Developing Mouse Nervous System', supported by National Institute of Mental Health (NIMH).

text: embryo 12.5,13.5,14.5 dpc

text: DH10B (T1 phage resistant)

text: Eye

characteristic: organ

text: Mus musculus

text: EcoR I

text: Not I

text: whole eye

text: Plasmid

text: pYX- Asc


text: live

text: Generic

text: The library was constructed according Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. Denatured RNA was size fractionated on a 1% agarose gel.First strand cDNA synthesis was primed with oligo-dT primer containing a Not I site .Double strand cDNA was size selected according to mRNA size fraction ,ligated with EcoR I adaptor , digested with NotI and then cloned directionally into pYX-Asc vector . The library tag sequence located between the Not I site and the polyA tail is TTATTGAAGT. This library was created for the University Iowa Brain Anatomy Project (BMAP): Gene Discovery in the Developing Mouse Nervous System, supported by National Institute of Mental Health (NIMH).

text: embryo 12.5,13.5,14.5 dpc

characteristic: dev-stage

text: DH10B (T1 phage resistant)

text: Eye

text: Mus musculus

text: EcoR I

text: Not I

text: whole eye

text: Plasmid

text: pYX- Asc


characteristic: External Id

text: live

text: Generic

text: The library was constructed according Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. Denatured RNA was size fractionated on a 1% agarose gel.First strand cDNA synthesis was primed with oligo-dT primer containing a Not I site .Double strand cDNA was size selected according to mRNA size fraction ,ligated with EcoR I adaptor , digested with NotI and then cloned directionally into pYX-Asc vector . The library tag sequence located between the Not I site and the polyA tail is CGAACTGAAT. This library was created for the University Iowa Brain Anatomy Project (BMAP): 'Gene Discovery in the Developing Mouse Nervous System', supported by National Institute of Mental Health (NIMH).

text: 9.5 and 10.5 dpc

text: DH10B (T1 phage resistant)

text: Head

text: Mus musculus

text: EcoR I

text: Not I

text: Upper Head

text: Plasmid

text: pYX-Asc


text: Chinese Academy of Sciences

text: live

text: Generic

text: Subtracted cDNA library prepared from mixed tissues of grass carp

text: 1 year

text: E.coli Electromax DH10B

text: Ctenopharyngodon idella

text: Male and female

characteristic: sex

text: Gill, liver

text: Grass carp mixed tissue library

text: PGEM-T


text: Children's Memorial Research Center

characteristic: INSDC center name

text: live

text: Generic

text: The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is TACCTCGAGA. Tissue was obtained from the Provasoli-Guillard National Center for Culture of Marine Phytoplankton (CCMP).

text: DH10B (Life Technologies) (T1 phage resistant)

text: Alexandrium tamarense

text: EcoR I

text: Not I

text: CCMP 1598

text: Plasmid

text: pT7T3-Pac (Pharmacia) with a modified polylinker


text: National Institute of Agrobiological Sciences

characteristic: INSDC last update

text: live

text: Generic

text: 5th instar larva day 3

text: Bombyx mori

text: Malpighian tubule


text: University of Incheon

text: live

text: Generic

text: Performed cDNA subtraction between unfertilized ovary and 10 DAP fruit

text: 10 days after pollination

text: E.coli Top10

text: Cucumis melo

text: cDNA subtraction library of 10 DAP fruit

characteristic: vector


text: Faculty of Veterinary medicine, Universite de Montreal

text: live

characteristic: INSDC status

text: Generic

text: granulosa and oocyte

characteristic: cell type

text: PCR fragment; cDNA library made from granulosa cells and oocytes of dominant follicles collected at day 5 of the estrous cycle that was subtracted from smaller than or equal to 4 mm follicles

text: dominant follicle

text: Bos taurus

text: female

text: Holstein

text: ovary

text: Bos taurus subtracted cDNA of granulosa cells and oocytes from dominant follicles (DF-SF)


text: Pioneer Hi-Bred International, Inc.

text: live

text: Generic

characteristic: NCBI submission model

text: W64A De*-B30

characteristic: cultivar

text: 16 dap

text: seed

text: Zea mays

text: endosperm

characteristic: tissue-type

text: Corn W64A De*-B30 endosperm cDNA library


text: University of Padova

text: live

text: Generic

characteristic: NCBI submission package

text: Mytilus galloprovincialis

text: Mytilus galloprovincialis haemolymph, gills, digestive gland, foot, adductor muscles and mantle


text: Marine Biological Laboratory

text: live

text: Generic

text: macrophages

text: Oncorhynchus mykiss

text: 12hr LPS Zap Express Library

text: pBK-CMV


text: live

text: Generic

text: 3 days after spinning

text: Bombyx mori

characteristic: strain

text: ovary


text: live

text: Generic

text: normalized library

characteristic: description

text: adult

text: Bombyx mori

characteristic: organism

text: Skuko x Ryuhaku

text: pheromone gland

text: NRPG


characteristic: INSDC first public

text: live

text: Generic

text: pupa day 5

text: Bombyx mori

text: Skuko x Ryuhaku

text: pheromone gland

characteristic: title


text: Nihon University, Biochemistry

characteristic: INSDC secondary accession

text: live

text: Generic

characteristic: SRA accession

text: early AGM stage

text: stage 46-52

characteristic: dev-stage

text: Xenopus laevis

text: AGM region

text: Xenopus laevis AGM region stage 46-52


characteristic: External Id

text: University of Minnesota

characteristic: INSDC center name

text: live

text: Generic

text: kidney epithelial cells

characteristic: cell-line

text: Monolayers of porcine kidney epithelial PK-15 cells (American Type Culture Collection, Manassas, VA) were infected with 10^5 TCID50 of each subtype of Swine influenza virus (SIV A/Swine/New Jersey/11/76 (H1N1), A/SW/MN-1480/00 (H1N2), and A/Swine/Minnesota/9088-2/98 (H3N2)). PK-15 cells were exposed to SIV or PBS for 1h at 37 degrees C, then the inoculum was removed, fresh media added, and monolayers incubated up to 2 days at 37 degrees C. Purified total RNA was treated with MessageClean (GenHunter Corp., Nashville, TN) to remove trace genomic DNA contamination and reversed transcribed. Differential display RT-PCR was used to identify 19 ESTs, which were cloned into pGEMTeasy.

text: Sus scrofa

text: EcoR V

text: plasmid

text: pGEMTeasy


text: Changchun Agriculture University

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Mus musculus

text: BALB/c

text: liver

text: Mus musculus BALB/c liver


text: National Cancer Institute / NIH

text: live

text: Generic

characteristic: NCBI submission model

text: Library is oligo-dT primed and directionally cloned Denatured RNA was size fractionated on a 1% agarose gel. First strand cDNA synthesis was primed with oligo-dT primer containing a Not I site. Double strand cDNA was size selected according tomRNA size fraction, ligated with EcoR I adaptor, digested with Not I and then cloned directionally into pYX-Asc vector. Average insert size 0.5-1Kb. Adaptors 5'(AATTCGGCACGAGG)3' and 5'd (CCTCGTGCCG)3'. 3' Linker sequence - GCGGCCGCTGAGAGCC T18. Sequencing primers 3'end: T3 promoter primer 5'd (ATTAACCCTCACTAAAGGGA)3'. 5' End: T7 promoter primer 5'd (TAATACGACTCACTATAGGG)3'. Library was constructed in the laboratory of M. Bento Soares. Average insert size 3-4kb Note: this is a NIH_MGC Library.

text: DH10B TonA

characteristic: lab-host

text: mixed

characteristic: organ

text: Homo sapiens

text: EcoRI

text: NotI

text: plasmid

text: pYX-Asc

characteristic: vector


text: National Cancer Institute / NIH

text: live

text: Generic

characteristic: NCBI submission package

text: Library is oligo-dT primed and directionally cloned Denatured RNA was size fractionated on a 1% agarose gel. First strand cDNA synthesis was primed with oligo-dT primer containing a Not I site. Double strand cDNA was size selected according tomRNA size fraction, ligated with EcoR I adaptor, digested with Not I and then cloned directionally into pYX-Asc vector. Average insert size 4-5Kb. Adaptors 5'(AATTCGGCACGAGG)3' and 5'd (CCTCGTGCCG)3'. 3' Linker sequence - GCGGCCGCTGAGAGCC T18. Sequencing primers 3'end: T3 promoter primer 5'd (ATTAACCCTCACTAAAGGGA)3'. 5' End: T7 promoter primer 5'd (TAATACGACTCACTATAGGG)3'. Library was constructed in the laboratory of M. Bento Soares. Note: this is a NIH_MGC Library

text: DH10B TonA

text: mixed

text: Homo sapiens

text: EcoRI

text: NotI

text: plasmid

text: pYX-Asc


text: California State University San Marcos

text: live

text: Generic

text: Emiliania huxleyi grown in Phosphate limited media (Guillard's F/50 media).

characteristic: description

text: Late log growth phase

text: Emiliania huxleyi

characteristic: strain

text: Preamplified custom cDNA library in pMAB58 (ResGen, Invitrogen Inc.)


text: The University of Georgia, Department of Plant Biology

characteristic: INSDC first public

text: live

text: Generic

characteristic: cultivar

text: The library was prepared from polyA+ RNA from leaves harvested from post-flowering, drought-stressed Sorghum bicolor, cv. B35. Double-stranded cDNA was cloned unidirectionally using the UniZap system from Stratagene. After amplification, the library was subtracted by re-association hybridization. Inserts can be excised with XhoI and EcoRI.

text: Post-flowering

text: ElectroMax DH10B (BRL)

text: Leaf

text: Sorghum bicolor

characteristic: organism

text: XhoI

text: EcoRI

text: Drought-stressed after flowering

text: Plasmid

text: pBlueScriptSK-


text: University of California

text: live

text: Generic

text: Assembly of sequence reads of overlapping cDNA clones

text: Asexual sporangia from hyphae grown on rye agar

text: Phytophthora infestans

text: SalI

text: NotI

text: Asexual sporangia undergoing zoosporogenesis

characteristic: title

text: plasmid


text: CSIRO Livestock Industries

text: live

text: Generic

text: Library made from pooled skin of adult female Hereford-Shorthorn.

text: Adult

characteristic: dev-stage

text: skin

text: Bos taurus

text: EcoRI

text: Xho I

text: female

characteristic: sex

text: Hereford Shorthorn

text: pooled

characteristic: tissue-type

text: Bos taurus skin cDNA library

text: Uni-ZAPXR


characteristic: External Id

characteristic: INSDC center name

text: live

text: Generic

text: The library was prepared from polyA+ RNA from leaves harvested from pre-flowering, drought-stressed Sorghum bicolor, cv. TX7000. Double-stranded cDNA was cloned unidirectionally using the UniZap system from Stratagene. After amplification, the library was subtracted by re-association hybridization. Inserts can be excised with XhoI and EcoRI.

text: Pre-flowering

text: ElectroMax DH10B (BRL)

text: Leaf

text: Sorghum bicolor

text: XhoI

text: EcoRI

text: Drought-stressed before flowering

text: Plasmid

text: pBlueScriptSK-


text: The Pennsylvania State University

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Subtractive library constructed using the CLONTECH PCR-select cDNA subtraction kit. Transgenic plants homozygous for the ag-1 mutation and carrying a 35S:AG-GR construct were generated. RNA was isolated from inflorescences with floral meristems and unopened floral buds and without open or older flowers from transgenic plants that were treated with dexamethasone for 3 days, and used for synthesis of the tester cDNA. RNA from the inflorescences of transgenic plants treated with a control solution without dexamethasone was used to synthesize the driver cDNA. It was observed that a 3-day treatment with dexamethasone can induce anther and carpel characteristics in the flowers of the ag-1 35S:AG-GR plants.

text: 4 week old plants

text: Arabidopsis thaliana

text: Landsberg erecta

text: Inflorescence lacking open or older flowers

text: Arabidopsis ag-1 35S:AG-GR forward subtraction library

text: The pT-Adv vector in the AdvanTAge Cloning Kit(CLONETECH)


text: Children's Memorial Research Center

text: live

text: Generic

characteristic: NCBI submission model

text: UI-D-GC1 is a normalized library derived from UI-D-GC0. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is TACCTCGAGA. Tissue was obtained from the Provasoli-Guillard National Center for Culture of Marine Phytoplankton (CCMP).

text: DH10B (Life Technologies) (T1 phage resistant)

characteristic: lab-host

text: Alexandrium tamarense

text: EcoR I

text: Not I

text: CCMP 1598

text: Plasmid

text: pT7T3-Pac (Pharmacia) with a modified polylinker


text: Washington University School of Medicine

text: live

text: Generic

text: The cDNA library was constructed by Keliang Tang, and Robert Cole at Washington University. cDNA was synthesized from poly mRNA using an oligo-dT primer containing a XhoI site. Following second strand synthesis, EcoRI adapters were ligated to the cDNA, and products were size-selected on sephacryl S500. The cDNA were directionally cloned into the EcoRI/XhoI prepared pBluescript II SK+ vector, and electroporated into GC10 Competent Cells(PGC). The library may contain a small percentage of host or bacterial contaminants.

text: Tachyzoite

text: GC10 Competent Cells(PGC)

text: Neospora caninum

text: EcoRI

text: XhoI

text: Nc-1 Tachyzoite cDNA Library 2

text: plasmid

text: pBluescript II SK+

characteristic: vector


text: live

text: Generic

characteristic: NCBI submission package

text: 1st strand of cDNA was synthesized with reverse transcriptase and oligo(dT) beads, then cDNA was amplified by PCR using modified SMART primers. The final cDNA was cloned in pAMP1 vector in annealing reaction with Uracil DNA Glycosylase (UDG). Library constructed by Y.Korshunova and M. Lovett. Library materials provided by Mills JC & Gordon JI.

text: adult

text: Mus musculus

text: Laser-captured isthmal cells from tox176 transgenic mice

text: Gastric Epithelial Progenitor 2

text: plasmid


text: Cardiff University

text: live

text: Generic

text: The library was prepared using protocols given by the supplier (Stratagene).

characteristic: description

text: Adult

text: Lumbricus rubellus

text: Whole worm

text: Earthworm Fluorantene Exposure Library

text: pBluescript II SK+


text: INRA Rennes

text: live

text: Generic

text: aphids inoculated on one-week old wheat germinations in test tube under non sterile conditions. experimental condition: short photoperiod (10-hr light/14-hr dark at 12oC during 12 days)

text: third instar nymph (L3)

text: Rhopalosiphum padi

text: SfiIA

text: SfiIB

characteristic: strain

text: whole insect

text: pDNR-LIB


text: Pisa University

characteristic: INSDC first public

text: live

text: Generic

text: country: Italy

text: vegetative mycelium

text: Dg2 (I2)

characteristic: isolate

text: Hordeum vulgare

text: Pyrenophora graminea

text: Pyrenophora graminea vegetative mycelium


text: Pisa University

text: live

text: Generic

text: country: Italy

text: vegetative mycelium

text: Dt4 (I4)

text: Hordeum vulgare

text: Pyrenophora teres

characteristic: organism

text: Pyrenophora teres vegetative mycelium


text: Universite Victor Segalen Bordeaux 2

text: live

text: Generic

text: P10 and P6 inferior colliculus commissural regions mRNA were isolated and subtracted by suppressive subractive hybridization technique. Subtracted cDNA were inserted into pT-Advantage plasmid and sequenced.

text: Brain

characteristic: organ

text: Mus musculus

text: Male and Female

text: Inferior Colliculus Commissural Region

text: Mouse P10/P6 Inferior Colliculus Subtracted Libraries

characteristic: title

text: Plasmid

text: pT-Advantage


text: live

text: Generic

text: Cloned unidirectionally. Poly (A)+ RNA was concentrated and purified using Dynabeads (Dynal) and mRNA eluted for 1ST strand synthesis. 1st strand cDNA created using MMLV RT(PowerScript, Clontech) and primed with oligo(dT) with XhoI site (primer CDSIII/3-XhoI ) and 5' SMART anchor added using chimeric DNA-RNA oligo (SMART-NotI-r-GGG). SMART-NotI-r-GGG was used in case a PCR using the SMART template was also desired. 15 PCR cycles were done using 1st strand and primers specific to SL1 leader sequence (SL1-NotI) and 3' end (XhoI-No-dT). dscDNA was digested using XhoI/NotI, fractionated on Chroma-spin 400 columns (Clontech) and ligated to digested pGEM -11zf(+) plasmid. Chemically competent cells were used as host. This library was provided by David Bird's lab (david_bird@ncsu.edu), of North Carolina State University.

text: adult

characteristic: dev-stage

text: Meloidogyne chitwoodi

text: XhoI

text: NotI

text: female

characteristic: sex

text: whole organism

text: Meloidogyne chitwoodi female SL1 pGEM

text: plasmid (ampicillin resistant)

text: pGEM-11zf(+) (Promega)


characteristic: External Id

text: Max-Planck-Institute for Plant Breeding Research

text: live

text: Generic

text: young leaf

text: Solanum tuberosum

text: EcoRI

text: EcoRI

text: leaf

characteristic: tissue-type

text: Solanum tuberosum young leaf

text: pBluescript


characteristic: INSDC center name

characteristic: INSDC last update

text: live

text: Generic

text: Cloned unidirectionally. Poly (A)+ RNA was concentrated and purified using Dynabeads (Dynal) and mRNA eluted for 1ST strand synthesis. 1st strand cDNA created using MMLV RT(PowerScript, CLontech) and primed with oligo(dT) with XhoI site (primer CDSIII/3-XhoI ) and 5' SMART anchor added using chimeric DNA-RNA oligo (SMART-NotI-r-GGG). SMART-NotI-r-GGG was used in case a PCR using the SMART template was also desired. 15 PCR cycles were done using 1st strand and primers specific to SL1 leader sequence (SL1-NotI) and 3' end (XhoI-No-dT). dscDNA was digested using XhoI/NotI, fractionated on Chroma-spin 400 columns (Clontech) and ligated to digested pGEM -11zf(+) plasmid. Chemically competent cells were used as host. This library was provided by David Bird's lab (david_bird@ncsu.edu), of North Carolina State University.

text: adult

text: Meloidogyne incognita

text: XhoI

text: NotI

text: female

text: whole organism

text: Meloidogyne incognita female SL1 pGEM

text: plasmid (ampicillin resistant)

text: pGEM-11zf(+) (Promega)


text: Institut National de la Recherche Agronomique

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: tissues: adipose tissue, brain, kidney, liver, muscle, ovary, testis, heart, hypothalamus, pancreas, skin, spleen, thymus, placenta, pituitary gland, seminal vesicle, small intestine, uterus, adrenals, bulbo uretral gland, cerebral trunk, epididymis, female gonad, gall-bladder, hippocampus, large intestine, male gonad, melanocytes, stomach, udder

text: from embryos to adults

text: Sus scrofa

text: mixed

text: Sus Scrofa library (scag)


text: live

text: Generic

text: Sus scrofa

text: mixed

text: Sus Scrofa library (scac)

characteristic: vector


text: University Of Tuebingen

text: live

text: Generic

characteristic: NCBI submission package

text: early appressorium induced fungal genes

text: early development

text: Funneliformis mosseae

text: early appressoria Glomus mosseae


text: live

text: Generic

characteristic: description

text: adult

characteristic: lab-host

text: Meloidogyne hapla

text: XhoI

text: NotI

text: female

text: whole organism

text: Meloidogyne hapla female SL1 pGEM

text: plasmid (ampicillin resistant)

text: pGEM-11zf(+) (Promega)


text: National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency

characteristic: INSDC first public

text: live

text: Generic

text: Total RNA was isolated from mature ovaries of abalone. The mRNA was further purified, reverse transcribed and cloned to pSPORT1

text: Ovaries

text: Haliotis asinina

text: Not I

text: Sal I

text: Female

text: Ovarian cDNA plasmid library

text: Plasmid


text: live

text: Generic

text: Total RNA was isolated from various stages of abalone testes. The mRNA was further purified, reverse transcribed and cloned to pSPORT1

text: Testis

text: Haliotis asinina

text: Not I

text: Sal I

text: Male

text: Testis cDNA plasmid library

text: Plasmid


text: live

text: Generic

text: Total RNA was isolated from mature ovaries of abalone. The mRNA was further purified for cDNA synthesis. Subtractive hybridization was carried out and cloned to pGEM-T Easy

text: Ovaries

text: Haliotis asinina

text: Female

text: Ovarian subtractive cDNA library

text: Plasmid

text: pGEM-T Easy


text: live

text: Generic

text: Total RNA was isolated from various stages of abalone testes. The mRNA was further purified for cDNA synthesis. Subtractive hybridization was carried out and cloned to pGEM-T Easy

text: Testes

text: Haliotis asinina

text: Male

text: Testis subtractive cDNA library

text: Plasmid

text: pGEM-T Easy


text: live

text: Generic

text: Subtractive cDNA library of ovaries using cDNA of testes as the driver was carried out. RACE-PCR was further performed and cloned to pGEM-T Easy.

text: Mature

text: E. coli JM109

text: Ovaries

text: Penaeus monodon

text: Female

text: Shrimp Ovarian Plasmid Library

text: Plasmid

text: pGEM-T Easy


text: Estonian Agricultural University, Institute of Experimental Biology

text: live

text: Generic

text: line 146-155-T, a derivative of Triticum timopheevii

characteristic: cultivar

text: 10-day-old plants, 24 hours after inoculation with Blumeria graminis subsp. tritici isolate No. 2

text: Triticum aestivum

text: leaves

text: Triticum aestivum RGA library


text: live

text: Generic

text: accession k-38555 (Vavilov Institute, St. Petersburg, Russia)

text: Triticum timopheevii

characteristic: organism

text: leaves

text: Triticum timopheevii RGA library


text: Virginia Bioinformatics Institute

text: live

text: Generic

text: Phytophthora sojae culture P6497

characteristic: cell-line

text: Harosoy

text: 48 hr. post infection stage

text: Glycine max

text: Phytophthora sojae-infected hypocotyl

text: Agriculture Canada Phytophthora sojae EST project

text: Plasmid

text: pBK-CMV


text: Virginia Bioinformatics Institute

text: live

text: Generic

text: Phytophthora sojae culture P6497

text: Harosoy

text: 48 hr. post infection stage

text: Glycine max

text: Phytophthora sojae-infected hypocotyl

text: USDA-IFAFS:Expression of Phytophthora sojae genes during infection and propagation

characteristic: title

text: Plasmid

text: pBK-CMV


text: Commonwealth Scientific and Industrial Research Organisation Division of Plant Industry

text: live

text: Generic

text: Himalaya

text: mRNA was prepared from endosperm tissues of the barley cultivar Himalaya. cDNA was synthesised from pooled 10, 12, and 15 dpa endosperm using Not I-oligo(dT)18 primer/adapter (Pharmacia Biotech), and then ligated to the Sal I-Not I site of _ZipLox vector (Life Technology) after adding a Sal I-Xho I adapter (Stratagene). Constructed by Shahjahan Ali and Bill Taylor.

text: developing endosperm tissue 10, 12, 15 dpa (days post anthesis)

characteristic: dev-stage

text: DH10B (Life Technology)

text: Hordeum vulgare subsp. vulgare

text: Sal I

text: Not I

text: endosperm

text: barley EST endosperm library

text: Phage

text: lambdaZipLox


text: live

text: Generic

text: Hartog

text: mRNA was prepared from endosperm tissues of the wheat cultivar Hartog. cDNA was synthesised from pooled 6, 8, and 10 dpa endosperm using Not I-oligo(dT)18 primer/adapter (Pharmacia Biotech), and then ligated to the Sal I-Not I site of lambdaZipLox vector (Life Technology) after adding a Sal I-Xho I adapter (Stratagene). Constructed by Shahjahan Ali and Bill Taylor.

text: developing endosperm tissue 6, 8, 10 dpa (days post anthesis)

text: DH10B (Life Technology)

text: Triticum aestivum

text: Sal I

text: Not I

text: endosperm

text: Hartog wheat EST endosperm library

text: Phage

text: lambdaZipLox


text: Horticulture Research International

text: live

text: Generic

text: Rebecca

text: A suppressive subtraction using a Clontech SSH kit was carried out using RNA from Stage 3 petals (open flowers) as the tester and Stage 2 petals (just open flower) as the driver. The resulting library should be enriched for genes expressed at stage 3.

text: expanded flower

text: flower

characteristic: organ

text: Alstroemeria peruviana

text: petal

text: Alstroemeria petal S3-S2 subtracted

text: plasmid

text: pGEMT (Promega)


characteristic: External Id

text: US Department of Agriculture (USDA), ARS, PSI

text: live

text: Generic

text: Bluecrop

text: cDNA clones from Vaccinium corymbosum cv. Bluecrop, RNA for preparation of library was extracted from flower buds collected in mid-winter from cold acclimated plants

text: Mature plants

text: Vaccinium corymbosum

text: Flower buds including bud scales

text: cDNA cold acclimated Bluecrop library

text: phagemid

text: pBluescript SK-


text: live

text: Generic

text: Bluecrop

text: cDNA clones from Vaccinium corymbosum cv. Bluecrop, RNA for preparation of library was extracted from flower buds collected in the fall from non acclimated plants

text: Mature plants

text: Vaccinium corymbosum

text: Flower buds including bud scales

characteristic: tissue-type

text: cDNA non acclimated Bluecrop library

text: phagemid

text: pBluescript SK-


text: Graduate School of Biotechnology, University of Korea

characteristic: INSDC center name

text: live

text: Generic

text: protonema

text: Physcomitrium patens

text: Physcomitrella patens subtraction


text: The Institute for Genomic Research

characteristic: INSDC last update

text: live

text: Generic

text: Coccidioides posadasii saprobic phase cDNA library, size fractionated cDNA > 4 kb

text: saprobic phase (mycelia)

text: E. coli DH10B, T1 phage resistant

text: Coccidioides posadasii

text: Not I

text: Eco RV

characteristic: strain

text: Coccidioides posadasii saprobic phase cDNA library, greater than 4kb

text: Plasmid

text: pExpress 1


text: The Institute for Genomic Research

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Coccidioides posadasii saprobic phase cDNA library, size fractionated cDNA 2 to 4 kb

text: saprobic phase (mycelia)

text: E. coli DH10B, T1 phage resistant

text: Coccidioides posadasii

text: Not I

text: Eco RV

text: Coccidioides posadasii saprobic phase cDNA library, 2 to 4 kb

text: Plasmid

text: pExpress 1


text: Department of Botany & Plant Sciences, University of California

text: live

text: Generic

characteristic: NCBI submission package

text: Ruby

text: Two trees with open-pollinated flowers in a mixed planting at UC Riverside Agricultural Operations were the source of tissue. Ovaries at anthesis were collected by Federici in March 2003. Tissues were snap frozen and then stored at -80C until further processing. Fenton (Close lab) purified RNA by the phenol method described in J. Japanese Soc. Hort. Sci. 1996. 64 (4): 809-814, purified poly(A) mRNA using a PolyATtrack mRNA Isolation System IV (Promega), produced a primary cDNA library using a lambda ZAP XR cDNA Synthesis Kit (Stratagene), then mass-excised 450,000 pfu from the primary library to produce a phagemid population. Phagemids were plated, plasmid DNA purified, cDNA clones archived, and DNA sequences determined bi-directionally using an ABI3730 at the Arizona Genomics Institute, University of Arizona (Kim, Kudrna, Wing, Yu). Chromatogram files were downloaded by FTP to UC Riverside (by Close), then processed at UC Riverside (by Wanamaker, Close lab) using the HarvEST pipeline (http://harvest.ucr.edu) to remove vector and cloning oligo sequences and various contaminants, and to trim to a high quality region. Sequences that retained a phred 17 region of at least 100 bases were deposited to GenBank.

text: 20 year old trees

text: E. coli TJC121

text: Citrus sinensis

text: EcoRI

text: XhoI

text: Ovary

text: Ruby Orange Ovary at Anthesis cDNA Library

text: Phagemid pBluescript SK(-)

text: Lambda Uni-ZAP XR, excised phagemid

characteristic: vector


text: live

text: Generic

text: Parent Washington Navel

text: Parent Washington Navel Orange trees on Troyer rootstock (UCR 16K) were the source of tissue. Trees, at UC Riverside Agricultural Operations, were planted October 12, 1992. In each of 17 reps one tree on Troyer rootstock was initially treated with Enzone, one with Alliette and Nemacure, and one was left untreated. These treatments were discontinued in 1998. At the time of sampling, there were differences in the apparent health and size of the trees on Troyer rootstock. Fall-flush shoots were sampled in early November 2002 to minimize the number of floral shoot meristems. Federici and Mu (Roose lab) harvested meristems only from trees that appeared to be healthy and had a large number of young shoot tips on the day of collection. The average weight of a meristem was about 2 mg. Federici noted that there were quite a few insects and signs of insect damage to the shoot tips. Mealy bugs, thrips and aphids were observed, plus a few very tiny fast moving insects that may have been mites or crawler stage of scale (although Federici did not see any mature scale). It was not difficult to avoid collecting most of these because they were easy to see with the dissecting microscope. It was harder to exclude the frass. Some frass was definitely retained in the samples. Tissues were snap frozen and then stored at -80C until further processing. Fenton (Close lab) purified RNA by the phenol method described in J. Japanese Soc. Hort. Sci. 1996. 64 (4): 809-814, purified poly(A) mRNA using a PolyATtrack mRNA Isolation System IV (Promega), produced a primary cDNA library using a lambda ZAP XR cDNA Synthesis Kit (Stratagene), then mass-excised one million pfu from the primary library to produce a phagemid population. Phagemids were plated, plasmid DNA purified, cDNA clones archived, and DNA sequences determined bi-directionally using an ABI3730 at the Arizona Genomics Institute, University of Arizona (Kim, Kudrna, Wing, Yu). Chromatogram files were downloaded by FTP to UC Riverside (by Close), then processed at UC Riverside (by Wanamaker, Close lab) using the HarvEST pipeline (http://harvest.ucr.edu) to remove vector and cloning oligo sequences and various contaminants, and to trim to a high quality region. Sequences that retained a phred 17 region of at least 100 bases were deposited to GenBank.

characteristic: description

text: 10 year old trees

text: E. coli TJC121

characteristic: lab-host

text: Citrus sinensis

text: EcoRI

text: XhoI

text: Shoot meristem

text: Washington Navel Orange Shoot Meristem cDNA Library

text: Phagemid pBluescript SK(-)

text: Lambda Uni-ZAP XR, excised phagemid


characteristic: INSDC first public

text: live

text: Generic

text: Clementina Fina Sodea

characteristic: cultivar

text: Rind separation is a prominent trait in Clementines. Kahn and Williams (UC Riverside) advised that the best Clementine for this trait is Fina Sodea. Lovatt (UC Riverside) provided advice on which portion of the peel would be most likely to contain transcripts related to rind separation. Federici (Roose lab) collected twenty fruit in November 2002 and with assistance from Mu (Roose lab) pulled off pieces of peel, and then cut the inner 2 mm of albedo that was adjacent to the fruit and snap froze this material in liquid nitrogen. The fruit were beginning to turn orange but were still mostly green and were moderately easy to peel, with some easier than others. Frozen tissues were stored at -80C until further processing. Fenton (Close lab) purified RNA by the phenol method described in J. Japanese Soc. Hort. Sci. 1996. 64 (4): 809-814, purified poly(A) mRNA using a PolyATtrack mRNA Isolation System IV (Promega), produced a primary cDNA library using a lambda ZAP XR cDNA Synthesis Kit (Stratagene), then mass-excised one million pfu from the primary library to produce a phagemid population. Phagemids were plated, plasmid DNA purified, cDNA clones archived, and DNA sequences determined bi-directionally using an ABI3730 at the Arizona Genomics Institute, University of Arizona (Kim, Kudrna, Wing, Yu). Chromatogram files were downloaded by FTP to UC Riverside (by Close), then processed at UC Riverside (by Wanamaker, Close lab) using the HarvEST pipeline (http://harvest.ucr.edu) to remove vector and cloning oligo sequences and various contaminants, and to trim to a high quality region. Sequences that retained a phred 17 region of at least 100 bases were deposited to GenBank.

text: 12 year old trees

text: E. coli TJC121

text: Citrus reticulata

text: EcoRI

text: XhoI

text: Albedo

text: Clementine Mandarin Albedo at Rind Separation cDNA Library

text: Phagemid pBluescript SK(-)

text: Lambda Uni-ZAP XR, excised phagemid


text: Virginia Bioinformatics Institute

text: live

text: Generic

characteristic: cell-line

text: 48 hr. post infection stage

text: Soybean plant

text: Phytophthora sojae

characteristic: organism

text: mycelium

text: USDA-IFAFS:Expression of Phytophthora sojae genes during infection and propagation_sHB

text: Plasmids

text: pBK-CMV


text: Virginia Bioinformatics Institute

text: live

text: Generic

text: mycelium

characteristic: dev-stage

text: synthetic medium

text: Phytophthora sojae

text: mycelium

text: USDA-IFAFS:Expression of Phytophthora sojae genes during infection and propagation_sMA

characteristic: title

text: Plasmids

text: pBK-CMV


characteristic: External Id

text: Virginia Bioinformatics Institute

text: live

text: Generic

text: mycelium

text: synthetic medium

text: Phytophthora sojae

text: mycelium

text: USDA-IFAFS:Expression of Phytophthora sojae genes during infection and propagation_sMC

text: Plasmids


text: Virginia Bioinformatics Institute

text: live

text: Generic

text: mycelium

text: synthetic medium

text: Phytophthora sojae

text: mycelium

characteristic: tissue-type

text: USDA-IFAFS:Expression of Phytophthora sojae genes during infection and propagation_sML

text: Plasmids


text: Virginia Bioinformatics Institute

characteristic: INSDC center name

text: live

text: Generic

text: mycelium

text: Phytophthora sojae

text: mycelium

text: Agriculture Canada Phytophthora sojae EST project_sMY

text: Plasmids

text: pBK-CMV


text: Virginia Bioinformatics Institute

characteristic: INSDC last update

text: live

text: Generic

text: Zoospore

text: synthetic medium

text: Phytophthora sojae

text: Zoospore

text: USDA-IFAFS:Expression of Phytophthora sojae genes during infection and propagation_sZG

text: Plasmids


text: Virginia Bioinformatics Institute

text: live

text: Generic

text: Free swimming

text: Phytophthora sojae

text: Zoospores

text: Agriculture Canada Phytophthora sojae EST project_sZO

text: Plasmids

text: pBK-CMV


text: Virginia Bioinformatics Institute

text: live

text: Generic

text: Free swimming

text: Phytophthora sojae

text: Zoospores

text: USDA-IFAFS:Expression of Phytophthora sojae genes during infection and propagation_sZS

text: Plasmids

text: pBK-CMV


text: North Carolina State University

text: live

text: Generic

text: Mycelial culture grown from 24 hrs to 6 days with varying Carbon and Nitrogen sources and concentrations.

text: mycelia

text: Trichoderma reesei

text: Not I/Sal I

characteristic: strain

text: T.reesei mycelial culture, Version 6 October 2003

text: Plasmid


text: National Institute of Agrobiological Sciences

text: live

characteristic: INSDC status

text: Generic

text: 5 weeks

text: Sus scrofa

text: male

characteristic: sex

text: lymphocyte

text: full-length enriched pig cDNA library, lymphocyte


text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: 5 weeks

text: Sus scrofa

text: male

text: olfactory bulb

text: full-length enriched pig cDNA library, olfactory bulb


text: Hunan Agricultural University

text: live

text: Generic

text: 1st strand cDNA primed with (GA)10ACTAGTCTCGAG(T)18, followed by second strand synthesis, and ligated to 5' adapter (5')-aattcggcacgag-3', 3'-gccgtgctc-5'. cDNA was cloned directionally (EcoRI/XhoI) into Stratagene Zap express lambda phage arms. Mass invivo excision done to obtain inserts in pBluescript SK(+/-) phagemid.

characteristic: description

text: 16 months

text: E. coli SOLR

text: Ctenopharyngodon idella

text: EcoRI

text: XhoI

text: Mixed male and female

text: hepart and spleen

text: Grass carp specific tissues cDNA library, Stratagene (cat. 200450)

text: phagemid

text: pBluescript SK(+/-)

characteristic: vector


text: Instituto de Investigaciones Biotecnologicas

characteristic: INSDC first public

text: live

text: Generic

text: PolyA(+) RNA was prepared using the QuickPrep Micro mRNA Purification Kit (Amersham Pharmacia Biotech). Synthesis of the cDNA first strand was done with Superscript II reverse transcriptase (Gibco BRL) and an oligo-dT-Not primer (5'-CCTGCGGCCGCT(18)-3'). Synthesis of the cDNA second strand was performed using the Klenow fragment of DNA polymerase with a spliced leader (SL) primer (5'-GATACAGTTTCTGTA-3'). After methylation with EcoRI methylase, phosphorylated EcoRI linkers (5'-ACGGAATTCGT-3') were ligated to the cDNA. The resulting cDNA mixture was then digested with NotI and EcoRI restriction enzymes, subjected to size fractionation on SizeSep400 Spun Columns (Pharmacia) to select fragments longer than 400 bp, and cloned into the dephosphorylated NotI/EcoRI sites of the vector.

text: trypomastigote

text: Trypanosoma cruzi

text: EcoRI

text: NotI

text: TcTR

text: Plasmid

text: pBluescript KS+


text: Instituto de Parasitologia y Biomedicina, CSIC

text: live

text: Generic

text: amastigote-like

text: Trypanosoma cruzi

text: EcoRI

text: NotI

text: TcAM

text: Plasmid

text: pBluescript SK+


text: University of Bristol

text: live

text: Generic

text: Mustang

characteristic: cultivar

text: 2 weeks old

text: Triticum aestivum

text: Heat stressed whole seedlings


text: National Institute on Aging/National Institutes of Health

text: live

text: Generic

text: Mouse cDNA project by the Laboratory of Genetics, National Institute on Aging (NIA), Intramural Research Program, NIH (http://lgsun.grc.nia.nih.gov/cDNA). This is a long-transcript enriched cDNA library (Ref. Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]). Total RNAs were obtained from Dr. Kenneth R. Boheler (National Institute on Aging, USA). ES cells were cultured without feeder cells in the presence of LIF and BRL-conditioned media. Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 14.2 ug of total RNA, treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-S. The products were purified by phenol/chloroform and Centricon 100. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pCMV-SPORT6 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 2.4 kb. The library was constructed by Yulan Piao.

text: R1 ES cells

characteristic: lab-host

text: Mus musculus

text: SalI

text: NotI

text: 129/Sv x 129/Sv-CP

text: NIA Mouse Undifferentiated ES Cell cDNA Library (Long 1)

text: plasmid (ampicillin resistant)

text: pCMV-SPORT6 (Invitrogen)


text: live

text: Generic

text: Mouse cDNA project by the Laboratory of Genetics, National Institute on Aging (NIA), Intramural Research Program, NIH (http://lgsun.grc.nia.nih.gov/cDNA). This is a long-transcript enriched cDNA library (Ref. Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]). EG cells were obtained from Dr. Brigid L.M. Hogan and RNA was prepared by Dr. Mark G. Carter (NIH/NIA-IRP). EG cells were cultured at 37. C, 5% CO2 in DMEM supplemented with 15% ES cell-qualified FBS, 0.1mM non-essential amino acids, 2 mM glutamine, penicillin/streptomycin, 1 mM sodium pyruvate, 0.1 mM beta-mercaptoethanol, and 10^7 units of LIF per liter. Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 2.5 ug of total RNA, treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-S. The products were double digested with Not1 and Sal1 enzymes, then purified by phenol/chloroform and Centricon 100. The cDNA mixture was subjected to a special subtraction procedure by Dr.Kazuhiro Kondo at AISIN Cosmos. Then the subtracted cDNAs were cloned into SalI/NotI site of pCMV-SPORT6 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 2.2kb. The library was constructed by Yulan Piao and Kazuhiro Kondo.

text: embryonic day 8

text: Mus musculus

characteristic: organism

text: SalI

text: NotI

text: male

text: NIA Mouse Embryonic Germ Cell cDNA Library (Long, subtracted)

text: plasmid (ampicillin resistant)

text: pCMV-SPORT6 (Invitrogen)


text: live

text: Generic

text: Mouse cDNA project by the Laboratory of Genetics, National Institute on Aging (NIA), Intramural Research Program, NIH (http://lgsun.grc.nia.nih.gov/cDNA). This is a long-transcript enriched cDNA library (Ref. Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]). Total RNAs were obtained from Dr. Janet Rossant and Tilo Kunath (Samuel Lunenfeld Research Institute, Canada). Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 4 ug of total RNA, treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-S. The products were purified by phenol/chloroform and Centricon 100. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pCMV-SPORT6 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 2.6 kb. The library was constructed by Yulan Piao.

characteristic: dev-stage

text: Mus musculus

text: SalI

text: NotI

text: B5/EGFP transgenic ICR mice

text: NIA Mouse Trophoblast Stem Cell cDNA Library (Long 1)

text: plasmid (ampicillin resistant)

text: pCMV-SPORT6 (Invitrogen)


text: live

text: Generic

text: Mouse cDNA project by the Laboratory of Genetics, National Institute on Aging (NIA), Intramural Research Program, NIH (http://lgsun.grc.nia.nih.gov/cDNA). This is a long-transcript enriched cDNA library (Ref. Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]). Total RNAs were obtained from Dr. Akihiro Umezawa (Keio University School of Medicine, Japan). Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 2.1 ug of total RNA, treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-S. The products were purified by phenol/chloroform and Centricon 100. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pCMV-SPORT6 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 3.0 kb. The library was constructed by Yulan Piao.

text: KUSA/A1 cells

text: Mus musculus

text: SalI

text: NotI

text: C3H/He mice

text: NIA Mouse Osteoblast cDNA Library (Long 1)

characteristic: title

text: plasmid (ampicillin resistant)

text: pCMV-SPORT6 (Invitrogen)


characteristic: External Id

text: live

text: Generic

text: Mouse cDNA project by the Laboratory of Genetics, National Institute on Aging (NIA), Intramural Research Program, NIH (http://lgsun.grc.nia.nih.gov/cDNA). This is a long-transcript enriched cDNA library (Ref. Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]). Total RNAs were obtained from Dr. Akihiro Umezawa (Keio University School of Medicine, Japan). Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 2.2 ug of total RNA, treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-S. The products were purified by phenol/chloroform and Centricon 100. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pCMV-SPORT6 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 2.5 kb. The library was constructed by Yulan Piao.

text: 9-15C cells

text: Mus musculus

text: SalI

text: NotI

text: C3H/He mice

text: NIA Mouse Mesenchymal Stem Cell cDNA Library (Long 1)

text: plasmid (ampicillin resistant)

text: pCMV-SPORT6 (Invitrogen)


text: live

text: Generic

text: Mouse cDNA project by the Laboratory of Genetics, National Institute on Aging (NIA), Intramural Research Program, NIH (http://lgsun.grc.nia.nih.gov/cDNA). This is a long-transcript enriched cDNA library (Ref. Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]). Total RNAs were obtained from Drs. Dennis Taub, Dan Longo (National Institute on Aging, USA), Jonathan Keller (National Cancer Institute, USA). Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 1.1 ug of total RNA, treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-S. The products were purified by phenol/chloroform and Centricon 100. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pCMV-SPORT6 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 2.2 kb. The library was constructed by Yulan Piao.

text: Age ~10 weeks old

text: Mus musculus

text: SalI

text: NotI

text: NIA Mouse Hematopoietic Stem Cell (Lin-/c-Kit-/Sca-1+) cDNA Library (Long 1)

text: plasmid (ampicillin resistant)

text: pCMV-SPORT6 (Invitrogen)


text: Max-Planck-Institut fuer Molekulare Genetik

text: live

text: Generic

text: OligodT primed and directionally cloned in pSport1 vector using a NotI (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC (T)15-3' and a SalI 5'- TCGACCCACGCGTCCG-3'adapters (Gibco BRL).

text: 26 hrs (neurula stage)

text: Escherichia coli, XL1 blue

text: Branchiostoma floridae

text: SalI, KpnI, EcoRI (5')

text: NotI, BamHI, HindIII (3')

text: wild type

text: whole embryo

characteristic: tissue-type

text: Amphioxus 26 hrs cDNA library (Name convention: BFL26 or MPMGp531)

text: Plasmid


text: Harbin Institute of Technology

characteristic: INSDC center name

text: live

text: Generic

text: myoblast

characteristic: cell type

text: Embryonic day 10 post-fertilization

text: Gallus gallus

text: Chicken Embryonic Myoblast cDNA Library


text: Max Planck Institute for Chemical Ecology

characteristic: INSDC last update

text: live

text: Generic

text: From pooled equal masses of corollas harvested from each Nicotiana attenuata, Nicotiana bigelovii and Nicotiana clevelandii mRNA was isolated and cloned as cDNA using Stratagene #200400 ZAP-cDNA Synthesis Kit.

text: flowering

text: E.coli

text: Nicotiana sp.

text: XhoI

text: EcoRI

text: flower

text: cDNAs of Nicotiana spec. genes activated in flowers

text: plasmid

text: pBLUESCRIPT SK (-)


text: live

characteristic: INSDC status

text: Generic

text: From isolated trichomes of Nicotiana attenuata mRNA was isolated and cloned as cDNA using Clontech #K1051-1 SMART cDNA Library Construction Kit.

text: elongated

text: E.coli

text: Nicotiana attenuata

text: SfiI

text: SfiI

text: trichomes

text: cDNAs of Nicotiana attenuata genes activated in trichomes

text: plasmid


text: School of Life Sciences and Biotechnology, Korea University

text: live

text: Generic

text: Gihobyeo

text: Using the gene specific primer based on the nucleotide of Copia type retrotransposon (OGRE-1 from O. grandiglumis Acc. No. 101154), a fragment (780 bp size) of the reverse transcriptase was amplified and cloned (T/A cloning).

text: Vegetative stage

text: Oryza sativa

text: leaf

text: Oryza sativa cv. Gihobyeo PCR and T/A cloning

text: Phagemid


text: live

text: Generic

text: Hwasungbyeo

text: Using the gene specific primer based on the nucleotide of Copia type retrotransposon (OGRE-1 from O. grandiglumis Acc. No. 101154), a fragment (780 bp size) of the reverse transcriptase was amplified and cloned.

text: Vegetative stage

text: Oryza sativa

text: leaf

text: Oryza sativa cv. Hwasungbyeo PCR and T/A cloning

text: Phagemid


text: live

text: Generic

text: Vegetative stage

text: Oryza punctata

characteristic: strain

text: leaf

text: Oryza punctata strain 100937 PCR and T/A cloning

text: Phagemid


text: live

text: Generic

characteristic: NCBI submission model

text: Vegetative stage

text: Oryza punctata

text: leaf

text: Oryza punctata strain 101389 PCR and T/A cloning

text: Phagemid


text: live

text: Generic

characteristic: NCBI submission package

text: Vegetative stage

text: Oryza punctata

text: leaf

text: Oryza punctata strain 101408 PCR and T/A cloning

text: Phagemid


text: live

text: Generic

characteristic: description

text: Vegetative stage

text: Oryza minuta

text: leaf

text: Oryza minuta strain 101080 PCR and T/A cloning

text: Phagemid


text: live

text: Generic

text: Vegetative stage

text: Oryza minuta

text: leaf

text: Oryza minuta strain 101083 PCR and T/A cloning

text: Phagemid

characteristic: vector


characteristic: INSDC first public

text: live

text: Generic

text: Vegetative stage

text: Oryza minuta

text: leaf

text: Oryza minuta strain 101084 PCR and T/A cloning

text: Phagemid