text: First Institute of Oceanography SOA,

text: live

text: Generic

text: Clontech's SMART cDNA Library Construction Kit (catalog number 634901) was used to construct the cDNA library. After cDNA synthesis by LD-PCR and size fractionation, the column eluent was then ligated into pTriplEx2 vector that has been digested with SfiI. The ligated DNA was transformed into BM25.8.

text: intestine

characteristic: organ

text: Apostichopus japonicus

text: SfiI

text: SfiI

text: intestine

text: Apostichopus japonicus Selenka intestine cDNA Library


status: PUBLIC

characteristic: External Id

text: USDA-ARS

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Leptinotarsa decemlineata

characteristic: organism

text: Suppressive subtractive hybridization screening of prediapausing Leptinotarsa decemlineata

characteristic: title


text: School of Life Science, Lanzhou University

text: live

text: Generic

text: late zygotene-pachytene

tag: attribute

characteristic: dev-stage

text: Lilium davidii var. willmottiae

text: anther

characteristic: tissue-type

text: Lilium davidii SSH cDNA sequence


text: Woods Hole Oceanographic Institution

text: live

text: Generic

text: ESTs from a subtractive library. Potentially higher expression in cDNA from mixed tissues of shell-diseased female lobster relative to an asymptomatic female lobster.

characteristic: description

text: Adult

text: Mixed: gill, hepatopancreas, muscle, ovary, heart, brain, branchiostegite

text: Homarus americanus

text: Female

characteristic: sex

text: Lobster shell disease state ESTs (library 1 of 4)


text: Woods Hole Oceanographic Institution

text: live

text: Generic

text: ESTs from a subtractive library. Potentially lower expression in cDNA from mixed tissues of shell-diseased female lobster relative to an asymptomatic female lobster.

text: Adult

text: Homarus americanus

text: Female

text: Lobster shell disease state ESTs (library 2 of 4)


text: Woods Hole Oceanographic Institution

text: live

text: Generic

text: ESTs from a subtractive library. One pool of cDNA was created from mixed tissues from two asymptomatic male lobsters and two asymptomatic female lobsters. A second pool was created from mixed tissues from two diseased male lobsters and two diseased female lobsters. This library contains ESTs with potentially higher expression in diseased animals.

text: Adult

text: Homarus americanus

text: Mixed

text: Lobster shell disease state ESTs (library 3 of 4)


text: Woods Hole Oceanographic Institution

text: live

text: Generic

text: ESTs from a subtractive library. This pool of cDNA was created from mixed tissues from two diseased male lobsters and two diseased female lobsters. This library contains ESTs with potentially higher expression in asymptomatic animals.

text: Adult

text: Homarus americanus

text: Mixed

text: Lobster shell disease state ESTs (library 4 of 4)


text: Shiraz University

text: live

text: Generic

text: 704 single cross

characteristic: cultivar

text: Seedlings of corn 704 were grown in growth chamber for 14 days. Isolation was carried out from leaves and by specific primers via PCR.

text: 14 days seedlings under normal condition

text: Leaf

text: Zea mays

text: Zea mays 704 single cross gene isolation method


text: MPI for Molecular Genetics

text: live

text: Generic

text: Brachionus plicatilis

text: mixed

text: whole animal

text: MS (sb101)


text: MPI for Molecular Genetics

text: live

text: Generic

text: Brachionus plicatilis

text: mixed

text: whole animal


text: MPI for Molecular Genetics

text: live

text: Generic

text: Brachionus plicatilis

text: female

text: whole animal

text: FRE (sb104)


text: MPI for Molecular Genetics

text: live

text: Generic

text: Brachionus plicatilis

text: female

text: resting eggs and embryos

text: REH (sb103)


text: MPI for Molecular Genetics

text: live

text: Generic

text: Brachionus plicatilis

text: female

text: resting eggs

text: RE (sb102)


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was prepared from approximately 200 eye-antennal imaginal discs and optic lobes dissected from both male and female wandering third instar larvae. Tissue was stored in RNAlater (Ambion). Total RNA was prepared using an RNA isolation kit (Promega) and RNA quality was verified using an Agilent bioanalyzer. Double-stranded cDNA was synthesized and amplified using the SMART PCR cDNA synthesis kit (Clontech). Two libraries were constructed on size-selected samples corresponding to transcripts above and below 1 kilobase and the majority of ESTs were sequenced from the larger-insert library (the average insert size for this library was 1.3 kb)

text: Late larval: wandering third instar larvae

text: Teleopsis dalmanni

text: Males and females

text: Eye-antennal imaginal disc

text: AFUX Teleopsis dalmanni Late larval eye-antennal disc EST library

text: pDNR-LIB

characteristic: vector


text: DOE Joint Genome Institute

text: live

text: Generic

text: Teleopsis dalmanni

text: Males and females

text: Eye-antennal imaginal disc

text: AFUY Teleopsis dalmanni Late larval eye-antennal disc EST library

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: Teleopsis dalmanni

text: Males and females

text: Eye-antennal imaginal disc

text: AFXP Teleopsis dalmanni Late larval eye-antennal disc EST library

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: Teleopsis dalmanni

text: Males and females

text: Eye-antennal imaginal disc

text: AGSB Teleopsis dalmanni Late larval eye-antennal disc EST library

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: Teleopsis dalmanni

text: Males and females

text: Eye-antennal imaginal disc

text: AGSC Teleopsis dalmanni Late larval eye-antennal disc EST library

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: Teleopsis dalmanni

text: Males and females

text: Eye-antennal imaginal disc

text: AGSF Teleopsis dalmanni Late larval eye-antennal disc EST library

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: Teleopsis dalmanni

text: Males and females

text: Eye-antennal imaginal disc

text: AHPZ Teleopsis dalmanni Late larval eye-antennal disc EST library

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: Teleopsis dalmanni

text: Males and females

text: Eye-antennal imaginal disc

text: AHSA Teleopsis dalmanni Late larval eye-antennal disc EST library

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: Teleopsis dalmanni

text: Males and females

text: Eye-antennal imaginal disc

text: AHSB Teleopsis dalmanni Late larval eye-antennal disc EST library

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was prepared from approximately 200 eye-antennal imaginal discs and optic lobes from both male and female pupae dissected 4-6 days from the initiation of pupation. Tissue was stored in RNAlater (Ambion). Total RNA was prepared using an RNA isolation kit (Promega) and RNA quality was verified using an Agilent bioanalyzer. Double-stranded cDNA was synthesized and amplified using the SMART PCR cDNA synthesis kit (Clontech). Two libraries were constructed on size-selected samples corresponding to transcripts above and below 1 kilobase and the majority of ESTs were sequenced from the larger-insert library (the average insert size for this library was 1.3 kb)

text: Mid-pupal: dissected 4-6 days following initiation of pupation

text: Teleopsis dalmanni

text: Males and females

text: Eye-antennal imaginal disc

text: ANPS Teleopsis dalmanni Mid-pupal eye-antennal disc EST library

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: Teleopsis dalmanni

text: Males and females

text: Eye-antennal imaginal disc

text: ANPT Teleopsis dalmanni Mid-pupal eye-antennal disc EST library

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: Teleopsis dalmanni

text: Males and females

text: Eye-antennal imaginal disc

text: ANPU Teleopsis dalmanni Mid-pupal eye-antennal disc EST library

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was prepared from approximately 200 eye-antennal imaginal discs and optic lobes from both male and female pupae dissected 1-3 days from the initiation of pupation. Tissue was stored in RNAlater (Ambion). Total RNA was prepared using an RNA isolation kit (Promega) and RNA quality was verified using an Agilent bioanalyzer. Double-stranded cDNA was synthesized and amplified using the SMART PCR cDNA synthesis kit (Clontech). Two libraries were constructed on size-selected samples corresponding to transcripts above and below 1 kilobase and the majority of ESTs were sequenced from the larger-insert library (the average insert size for this library was 1.2 kb)

text: Early pupal: dissected 1-3 days following initiation of pupation

text: Teleopsis dalmanni

text: Males and females

text: Eye-antennal imaginal disc

text: AOBT Teleopsis dalmanni Early pupal eye-antennal disc EST library

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: Teleopsis dalmanni

text: Males and females

text: Eye-antennal imaginal disc

text: AOBU Teleopsis dalmanni Early pupal eye-antennal disc EST library

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: Teleopsis dalmanni

text: Males and females

text: Eye-antennal imaginal disc

text: AOTB Teleopsis dalmanni Mid-pupal eye-antennal disc EST library

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: Teleopsis dalmanni

text: Males and females

text: Eye-antennal imaginal disc

text: AOTC Teleopsis dalmanni Mid-pupal eye-antennal disc EST library

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was prepared from approximately 200 eye-antennal imaginal discs and optic lobes dissected from both male and female wandering third instar larvae. Tissue was stored in RNAlater (Ambion). Total RNA was prepared using an RNA isolation kit (Promega) and RNA quality was verified using an Agilent bioanalyzer. Double-stranded cDNA was synthesized and amplified using the SMART PCR cDNA synthesis kit (Clontech). Two libraries were constructed on size-selected samples corresponding to transcripts above and below 1 kilobase and the majority of ESTs were sequenced from the larger-insert library (the average insert size for this library was 1.3 kb)

text: Late larval: wandering third instar larvae

text: Teleopsis dalmanni

text: Males and females

text: Eye-antennal imaginal disc

text: CAXN Teleopsis dalmanni Late larval eye-antennal disc EST library

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: Teleopsis dalmanni

text: Males and females

text: Eye-antennal imaginal disc

text: CAXO Teleopsis dalmanni Early pupal eye-antennal disc EST library

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: Teleopsis dalmanni

text: Males and females

text: Eye-antennal imaginal disc

text: CAZC Teleopsis dalmanni Mid-pupal eye-antennal disc EST library

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: Teleopsis dalmanni

text: Males and females

text: Eye-antennal imaginal disc

text: CZAB Teleopsis dalmanni Early pupal eye-antennal disc EST library

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: Teleopsis dalmanni

text: Males and females

text: Eye-antennal imaginal disc

text: CZAC Teleopsis dalmanni Late larval eye-antennal disc EST library

text: pDNR-LIB


text: INRA-SCRIBE

text: live

text: Generic

text: E.coli TOP10

characteristic: lab-host

text: Dicentrarchus labrax

text: gills

text: Aquafirst Sea Bass SSH selection pathogen gill (sbrg07)

text: plasmid

text: pCR 2.1


text: INRA-SCRIBE

text: live

text: Generic

text: E.coli TOP10

text: Dicentrarchus labrax

text: head kidney

text: Aquafirst Sea Bass SSH selection confinement head kidney (sbry03)

text: plasmid

text: pCR 2.1


text: INRA-SCRIBE

text: live

text: Generic

text: E.coli TOP10

text: Dicentrarchus labrax

text: gills

text: Aquafirst Sea Bass SSH selection pathogen gill (sbrg08)

text: plasmid

text: pCR 2.1


text: INRA-SCRIBE

text: live

text: Generic

text: E.coli TOP10

text: Dicentrarchus labrax

text: head kidney

text: Aquafirst Sea Bass SSH selection confinement head kidney (sbry04)

text: plasmid

text: pCR 2.1


text: Uppsala University

text: live

text: Generic

text: E.coli TOP10

text: Dicentrarchus labrax

text: brain

text: confinement brain late (sbub03)

text: plasmid

text: pCR 2.1


text: Uppsala University

text: live

text: Generic

text: E.coli TOP10

text: Dicentrarchus labrax

text: brain

text: confinement brain late (sbub04)

text: plasmid

text: pCR 2.1


text: Uppsala University

text: live

text: Generic

text: E.coli TOP10

text: Dicentrarchus labrax

text: brain

text: pathogen brain early (sbub06)

text: plasmid

text: pCR 2.1


text: Uppsala University

text: live

text: Generic

text: E.coli TOP10

text: Dicentrarchus labrax

text: brain

text: confinement brain early (sbub02)

text: plasmid

text: pCR 2.1


text: Uppsala University

text: live

text: Generic

text: E.coli TOP10

text: Dicentrarchus labrax

text: brain

text: pathogen brain late (sbub07)

text: plasmid

text: pCR 2.1


text: Uppsala University

text: live

text: Generic

text: E.coli TOP10

text: Dicentrarchus labrax

text: brain

text: pathogen brain late (sbub08)

text: plasmid

text: pCR 2.1


text: Uppsala University

text: live

text: Generic

text: E.coli TOP10

text: Dicentrarchus labrax

text: brain

text: confinement brain early (sbub01)

text: plasmid

text: pCR 2.1


text: Uppsala University

text: live

text: Generic

text: E.coli TOP10

text: Dicentrarchus labrax

text: brain

text: pathogen brain early (sbub05)

text: plasmid

text: pCR 2.1


text: Agricultural Research Service, USDA

text: live

text: Generic

text: Merozoites cultured in vivo were collected at 96 hrs. post-infection and were washed and filtered prior to RNA extraction.

text: merozoite

text: Eimeria maxima

text: Tysons

characteristic: strain

text: Eimeria maxima merozoite life stage cDNA library

text: Phage

text: Uni-ZAP XR


characteristic: External Id

text: Kazusa DNA Research Institute;

text: live

text: Generic

text: Micro-Tom

text: RNA samples for cDNA library construction were prepared from six sources; roots from 3-month-old tomato (Solanum lycopersicum cv Micro-Tom) plants with flowers, roots from 3-month-old tomato plants without flowers, and Fusarium (F.oxyporum race2)-treated tomato roots (2-month-old) harvested 1 day and 1, 2, and 3weeks after infection. A full-length enriched cDNA library was constructed from a mixture of the RNAs by using a CAP-trapper method (Carninci and Hayashizaki, Methods Enzymol., 303: 19-44, 1999).

text: Solanum lycopersicum

text: root

text: Solanum lycopersicum cv Micro-Tom root


text: Nagoya University

characteristic: INSDC center name

text: live

text: Generic

text: synergid cell

characteristic: cell type

text: mature flower

text: Torenia fournieri

text: mature ovule

text: synergid cell cDNA library


text: University of Alaska Fairbanks

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: synonym: Lophonetta specularioides. specimen_voucher: UAM:KGM 1278, 1288, 1290, 1292, 1293, 1310. country: Peru, Junin, 4218-4605 m. lat_lon: 11.2 S 76.2 W. collection_date: 14-19 June 2006.

text: Lophonetta specularioides

text: bone marrow

text: Lophonetta specularioides bone marrow cDNA library

text: pDNR-LIB


text: National Institute for Plant Genome Research

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Transgenic and wild type

text: Subtractive cDNA library made between wild type and transgenic tobacco (Nicotiana tabacum).

text: Seedling

text: Nicotiana tabacum

text: Subtracted cDNA library of transgenic and wild type tobacco


text: Shanghai Ocean University

text: live

text: Generic

characteristic: NCBI submission package

text: Liver

characteristic: organ

text: Sinonovacula constricta

characteristic: organism

text: Agamaki Clam

text: Liver

text: Agamaki Clam liver normalized cDNA library

text: Plasmid

text: pGEM-T vector


text: Wellcome Trust Sanger Institute

text: live

text: Generic

text: bloodstream stage

characteristic: dev-stage

characteristic: isolate

text: Trypanosoma congolense

text: Trypanosoma congolense isolate IL300 bloodstream stage

characteristic: title


text: Berry College

text: live

text: Generic

text: Isolated by Representational Difference Analysis and cloned into Invitrogen pCR II-TOPO cloning vector.

characteristic: description

text: Adult

text: Exaiptasia diaphana

text: Aiptasia/ lindane responses

text: Invitrogen pCR II-TOPO


text: Huazhong Agricultural University

text: live

text: Generic

text: Petunia x hybrida

text: Subtracted library of Pettunia between double and single flower trait


text: Trinity College Dublin

text: live

text: Generic

text: cDNA from young leaves of Lathyrus odoratus. Library contains a small percentage of contamination from cDNAs from Danio rerio (zebrafish).

text: Leaf

text: Lathyrus odoratus

text: Lathyrus odoratus leaf


text: University of Georgia

text: live

text: Generic

text: Tifrunner

characteristic: cultivar

text: Total RNAs were isolated from developing embryos with Trizol (Invitrogen). The normalized cDNA libraries were constructed with Smart cDNA Library Construction Kit (Clontech). The inserts were cloned into pPCR-Script-Amp-SK+ vector after Srf I digestion. The clones were transformed to the host cell DH10B and selected using chloramphenicol.

text: mid-maturation seed

text: Arachis hypogaea

text: SrfI

text: SrfI

text: developing embryo

characteristic: tissue-type

text: Normalized cDNA library from developing embryos of peanut

text: pPCR-Script-Amp-SK+ (Strategene)

characteristic: vector


characteristic: External Id

text: National Institute of Oceanography

text: live

text: Generic

text: Suppression subtractive hybridization was carried out. The products were then cloned into pGEMTeasy vector.

text: 15 day postlarvae

text: Penaeus monodon

text: whole animal

text: Vibrio harveyi challenged Tiger shrimp postlarvae cDNA library

text: pGEMTeasy


text: University of Florida/IFAS

characteristic: INSDC center name

text: live

text: Generic

text: adult

text: Culex nigripalpus

text: female

characteristic: sex

text: abdomen

text: Mosquito female adult abdomen


text: Wellcome Trust Sanger Institute

characteristic: INSDC first public

text: live

text: Generic

text: procyclic stage

text: Trypanosoma congolense

text: Trypanosoma congolense isolate IL300 procyclic stage


text: Wellcome Trust Sanger Institute

characteristic: INSDC last update

text: live

text: Generic

text: epimastigote stage

text: Trypanosoma congolense

text: Trypanosoma congolense isolate IL300 epimastigote stage


text: Wellcome Trust Sanger Institute

text: live

characteristic: INSDC status

text: Generic

text: metacyclic stage

text: Trypanosoma congolense

text: Trypanosoma congolense isolate IL300 metacyclic stage


text: Wellcome Trust Sanger Institute

text: live

text: Generic

characteristic: NCBI submission model

text: metacyclic stage

text: Trypanosoma congolense

text: Trypanosoma congolense isolate IL300 normalised metacyclic stage


text: Wellcome Trust Sanger Institute

text: live

text: Generic

characteristic: NCBI submission package

text: bloodstream stage

text: Trypanosoma congolense

text: Trypanosoma congolense isolate IL300 normalised bloodstream stage


text: Huazhong Agricultural University

text: live

text: Generic

text: Ponkan Mandarin

text: different developments of flower and fruit

text: Escherichia coli DH5a

characteristic: lab-host

text: Floral Organs and Fruitlets

characteristic: organ

text: Citrus reticulata

text: hermaphrodite

text: E-gan NO.1

characteristic: strain

text: Bud, ovary, fruitlet

text: Citrus Floral Organs and Fruitlets cDNA Library

text: Plasmid

text: PDNR-LIB


text: University of Muenster

text: live

text: Generic

text: Total RNA extracted from different growth stages of S. typhi was equally combined and size fractionated (10-500nt) on 8% denaturing PAGE gel (7M urea, 1XTBE buffer). Passive elution was performed in 0.3M NaOAc (pH 5.3) overnight at 4oC. Subsequently, 5 ug size fractionated RNA was treated with tobacco acid pyrophosphatase (Epicenter) for 1 hr at 37oC and C-tailed with poly-A polymerase (Invitrogen) for 2 hr at 37oC. A 5' DNA Sal-1 adaptor (5'-CAACGCGTCGACTACGTGAGATTTGAGGTTC-3') was then ligated to the RNA using T4 RNA ligase (Fermentas) at 4oC overnight. First-strand cDNA synthesis was performed using the Transcriptor reverse transcriptase (Roche) and Not-1 DNA oligonucleotide (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCGGGGGGGGGGGGGGG-3'). The RNA-cDNA mix was treated with RNase A and single strand PCR reaction using Sal-1 primer was performed. The PCR product was then double digested by Sal-1 and Not-1 restriction endonucleases and ligated into the pSPORT1 vector (Invitrogen), followed by transformation into E. coli Top10 electrocompitent cells (Invitrogen).

text: clinical isolate USM05

characteristic: isolate

text: Salmonella enterica subsp. enterica serovar Typhi

characteristic: organism

text: cDNA library representing npcRNA from S. Typhi

text: Plasmid

text: pSport


text: Instituto Biologia Vegetal y Biotecnologia, Universidad de Talca

text: live

text: Generic

text: 1 year old seedlings, open-pollinated trees

characteristic: dev-stage

text: Stem

text: Pinus radiata

text: Xylem

text: Pinus radiata gravitropic stem response

characteristic: title

text: pSC-A-amp/kan


text: Seoul National University

text: live

text: Generic

text: Stratagene's cDNA Synthesis Kit (catalog number 200401), ZAP Express Vector Kit (catalog number 239211), and Gigapack III Gold Packaging Extract (catalog number 200201) were used to construct the cDNA library. After second-strand cDNA synthesis, adapter ligation, and size fractionation, the column eluent was then ligated into Stratagene's UniZAP XR vector that has been digested with EcoRI and XhoI, and phosphorylated by Stratagene. The ligated DNA was packaged using Stratagene's Gigapack III Gold Packaging Extract and the pBluescript SK phagemid and insert was circularized to phagemid by in vivo excision.

characteristic: description

text: Capsicum annuum

text: EcoRI

text: XhoI

text: Leaf

characteristic: tissue-type

text: Hotpepper under oxidative stress

text: pBluescript SK


characteristic: External Id

text: Stazione Zoologica Anton Dohrn

text: live

text: Generic

text: The Pooled Depth cDNA library was obtained from samples collected by SCUBA diving from a meadow located in Lacco Ameno, Ischia (Gulf of Naples), at 5 and 25 meters depth. Total RNA was isolated from pooled young leaf tissue and meristematic portions of shoots using a CTAB method. Lithium Chloride was used for precipitation of total RNA. The cDNA was synthesized by vertis Biotechnologie AG, Freising, Germany. cDNA was not-directionally ligated into the Eco RI and Bam HI sites of the vector plasmid pBSII sk+ (2961 bp). Fragments length distribution was from 650bp to 2000bp (max conc. from 800bp to 1200bp). Randomly selected clones from the library were isolated from plasmids and sequenced. T7 primer was used for sequencing. Sequencing was performed both at the Stazione Zoologica Anton Dohrn (Naples, Italy) and at the Max Planck Institute of Molecular Genetics (Berlin, Germany), thanks to Marine Genomics Europe TP grants. The EST library has been processed using pregap4 (Staden package) and cross_match (phrap system) and is stored in the Dr.Zompo database, available at http://drzompo.uni-muenster.de/

text: adult

text: Posidonia oceanica

text: Eco RI

text: Bam HI

text: hermaphrodite

text: leaf

text: Plasmid

text: pBSII sk+

characteristic: vector


text: The General Environmental Technos Co., Ltd

characteristic: INSDC center name

text: live

text: Generic

text: variety: turkestanica

text: Salvia sclarea

text: Salvia sclarea turkestanica lambda ZAP cDNA


text: The life science college of Shihezi University

characteristic: INSDC first public

text: live

text: Generic

text: We used Triol to extract RNA from normal liver and disease liver which is caused by Echinococcus granulosus. The sample had been took from patient who was infested by Echinococcus granulosus ten years ago. Then we used methods of construction of SSH cDNA Lib to get these EST sequence.

text: Liver

text: Homo sapiens

text: male

characteristic: sex

text: SSH library of human liver disease caused by Echinococcus granulosus

text: Plasmid

text: pGM-T


text: Rubber Reseach Institute

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: The libraries were constructed by suppression substractive hybridization method

text: 16 year old tree

text: Hevea brasiliensis

text: Latex

text: cDNA libraries of the latex from healthy and TPD rubber trees


text: The Jackson Laboratory

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Using SSH, cDNA from morulae was subtracted by cDNA from 4-cell embryos. This library is enriched in transcripts specifically expressed during genome activation in bovine embryos.

text: cleavage

text: Bos taurus

text: bcan


text: Universidad Nacional Autonoma de Mexico

text: live

text: Generic

text: Negro Jamapa 81

characteristic: cultivar

text: Total RNA was prepared from P. vulgaris nodules, 15 days post inoculation with Rhizobium tropici CIAT 899. Poly (A+) RNA was obtained from total RNA using oligo (dT) cellulose. Poly (A+) RNA was reverse transcribed and cloned in the Uni-Zap XR vector (Stratagene). Plasmids containing cDNAs were then excised from the Uni-Zap XR vector and propagated in SOLR cells. All sequences were searched for vector segments to check for insert integrity. The sequences contain a minimum of 90 high quality bases and have been trimmed to remove vector and adaptor sequences.

text: SOLR

characteristic: lab-host

text: Nodule

characteristic: organ

text: Phaseolus vulgaris

text: EcoRI

text: XhoI

text: Phaseolus vulgaris Nodule EST library

text: Plasmid

text: pBluescript SK(+)


text: College of Biological Sciences and Biotechnology, Beijing Forestry University

text: live

text: Generic

text: Ammopiptanthus mongolicus

characteristic: organism

text: seedling

text: Ammopiptanthus mongolicus seedling PEG and cold-induced transcript-derived fragment (TDF)


text: Israel Oceanographic and Limnological Research, National Institute of Oceanography

text: live

text: Generic

text: adult

characteristic: dev-stage

text: Botryllus schlosseri

text: hermaphrodite

text: Botryllus subtraction EST libraries

text: Plasmid


text: School of Life Science, Shanghai University

text: live

text: Generic

text: inbred line W22

text: We constructed a normalized full-length Maize endosperm cDNA Library

text: Escherichia coli DH10B

text: endosperm

text: Zea mays

text: SfiI

text: SfiI

text: endosperm

text: a normalized full-length Maize endosperm cDNA library

characteristic: title

text: Plasmid

text: pBluescript SK-


text: Boyce Thompson Institute for Plant Research

text: live

text: Generic

text: E.coli SOLR

text: Solanum lycopersicum

text: EcoRI

text: XhoI

text: seed

text: Tomato Seed Library [B]

text: pBluescript SK


text: live

text: Generic

text: E. coli SOLR

text: Solanum lycopersicum

text: EcoRI

text: XhoI

text: seed

text: Tomato Seed Library [C]

text: pBluescript SK


text: live

text: Generic

text: E. coli SOLR

text: Solanum lycopersicum

text: EcoRI

text: XhoI

text: seed

text: Tomato Seed Library [D]

text: pBluescript SK


text: National Institute of Vegetable and Tea Science

text: live

text: Generic

text: Camellia sinensis

text: Camellia sinensis library (Taniguchi F)


text: Institute of Marine Research

text: live

text: Generic

text: Tissue obtained from coastal cod

characteristic: description

text: Gadus morhua

text: Embryo

text: Atlantic cod embryo library CE

text: pBluescript


text: Institute of Marine Research

text: live

text: Generic

text: Tissue obtained from coastal cod

text: Gadus morhua

text: Embryo

characteristic: tissue-type

text: Atlantic cod embryo library CEM

text: pBluescript


characteristic: External Id

text: Institute of Marine Research

text: live

text: Generic

text: Tissue obtained from coastal cod

text: Gadus morhua

text: Brain

text: Atlantic cod brain library CHJ

text: pBluescript


text: Institute of Marine Research

text: live

text: Generic

text: Tissue obtained from coastal cod

text: Gadus morhua

text: Head kidney

text: Atlantic cod head kidney library CHO

text: pBluescript

characteristic: vector


text: Institute of Marine Research

characteristic: INSDC center name

text: live

text: Generic

text: Tissue obtained from coastal cod

text: Gadus morhua

text: Pituitary gland

text: Atlantic cod pituitary library CHY

text: pBluescript


text: Institute of Marine Research

characteristic: INSDC first public

text: live

text: Generic

text: Tissue obtained from coastal cod

text: Gadus morhua

text: Liver

text: Atlantic cod liver library CLE

text: pBluescript


text: Institute of Marine Research

text: live

text: Generic

text: Tissue obtained from coastal cod

text: Gadus morhua

text: Spleen

text: Atlantic cod spleen library CMI

text: pBluescript


text: Institute of Marine Research

text: live

text: Generic

text: Tissue obtained from coastal cod

text: Gadus morhua

text: Ovary

text: Atlantic cod ovary library COV

text: pBluescript


text: Institute of Marine Research

text: live

text: Generic

text: Tissue obtained from coastal cod

text: Gadus morhua

text: Pylorus

text: Atlantic cod pylorus library CPY

text: pBluescript


text: Institute of Marine Research

text: live

text: Generic

text: Tissue obtained from coastal cod

text: Gadus morhua

text: Spleen

text: Atlantic cod stimulated spleen library CSMI

text: pBluescript


text: Institute of Marine Research

text: live

text: Generic

text: Tissue obtained from coastal cod

text: Gadus morhua

text: Intestine

text: Atlantic cod intestine library CTA

text: pBluescript


text: Institute of Marine Research

text: live

text: Generic

text: Tissue obtained from coastal cod

text: Gadus morhua

text: Testis

text: Atlantic cod testis library CTE

text: pBluescript


text: Institute of Marine Research

text: live

text: Generic

text: Tissue obtained from coastal cod

text: Gadus morhua

text: Embryo

text: Atlantic cod embryo library RZEAA

text: pBluescript


text: University of Victoria

text: live

text: Generic

text: Tissue contributors: Brendan Connors, Salmon Coast Field Station, Simoom Sound

text: whole

text: Caligus clemensi

text: whole

text: ccleevs mixed_tissue Caligus clemensi cDNA


text: University of Victoria

text: live

text: Generic

text: Tissue contributors: James Bron, Sarah Barker - University of Stirling, Scotland, UK

text: mixed tissue

text: Lernaeocera branchialis

text: mixed tissue

text: lbraevt mixed_tissue Lernaeocera branchialis cDNA


text: National Institute of Agrobiological Sciences

text: live

text: Generic

text: country: Japan:Kagoshima, Yakushima island

text: Hodotermopsis sjostedti

text: whole body (without the digestive tract, containing symbiont protozoa) of the pseudergate caste

text: Hodotermopsis sjoestedti substracted cDNA library


text: Kansas State University

text: live

text: Generic

text: First instars were given a 2 week acquisition access period (AAP) on Maize mosaic virus-infected corn plants and were then moved to healthy corn plants for three days. The resulting 4th and 5th instar nymphs were collected, guts dissected, and RNA extracted.

text: 4th and 5th instar nymphs

text: Peregrinus maidis

text: Sfi I

text: Hawaiian

characteristic: strain

text: gut

text: Corn planthopper gut library

text: plasmid

text: pDNR-LIB vector (Clontech)


text: College of Animal Science and Technology, SiChuan Agricultural University

characteristic: INSDC last update

text: live

text: Generic

text: preadipocyte

characteristic: cell type

text: primary

characteristic: cell-line

text: The primary preadipocyte induced differentiation Library is derived from the suppression subtractive hybridization between before induced differentiation(as driver) and after induced differentiation(as tester)

text: Sus scrofa

text: Male

characteristic: sex

text: Large White-Rongchang crossbreed

text: subcutaneous adipose

text: primary preadipocyte induced differentiation SSH-1 Library


text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: preadipocyte

text: primary

text: The primary preadipocyte induced differentiation Library is derived from the suppression subtractive hybridization between after induced differentiation(as driver) and before induced differentiation(as tester)

text: Sus scrofa

text: Male

text: Large White-Rongchang crossbreed

text: subcutaneous adipose

text: primary preadipocyte induced differentiation SSH-2 Library


text: The Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences

text: live

text: Generic

text: adult

characteristic: lab-host

text: Ganoderma lucidum

characteristic: organism

text: Fruiting body

text: Ganoderma lucidum Fruiting body cDNA Library

text: Plasmid

text: pDNR-LIB


text: Dalian Fisheries University

text: live

text: Generic

text: Strongylocentrotus intermedius

text: gonad

text: Sea urchin Strongylocentrotus intermedius gonad cDNA Library

characteristic: title


text: Universite Jean Monnet

text: live

text: Generic

characteristic: cultivar

text: Flower

characteristic: organ

text: Lavandula angustifolia

text: Lavandula angustifolia Flower library


characteristic: External Id

text: Oviedo University

text: live

text: Generic

text: Differential EST expression both in ABA treated cotyledons (PRAABA) and in control cotyledon (PRACABA)

characteristic: description

text: cotyledon

text: Pinus radiata

text: ABAVsCABA


text: Oviedo University

characteristic: INSDC center name

text: live

text: Generic

text: EST expressed in Pinus radiata cotyledons both under BA treatment (PRABA) and without hormone induction

text: Cotyledon

text: Pinus radiata

text: BAVsControl

text: pGEM-T easy

characteristic: vector


text: Auburn University

characteristic: INSDC first public

text: live

text: Generic

text: Whole body

text: Musca domestica

text: EcoR I

text: Xho I

text: House Fly cDNA library

text: Plasmid

text: pBluescript SK


text: Fujian Agriculture and Forestry University

characteristic: INSDC last update

text: live

text: Generic

text: Pulp

text: Color break stage and half yellow stage

characteristic: dev-stage

text: Carica papaya

text: Fruit

characteristic: tissue-type

text: Differential gene expression of papaya different ripening fruit


text: College of life science, Northwest A&F University

text: live

text: Generic

text: seed filling period

text: E.coli XL1-Blue

text: Fagopyrum tataricum

text: SFiIA

text: SFiIB

characteristic: strain

text: seed

text: seeds filling period cDNA library of tartary buckwheat

text: phage

text: Lambda TripIEX2


text: College of Fisheries, Karnataka Veterinary, Animal and Fisheries Sciences University

text: live

characteristic: INSDC status

text: Generic

text: Whole animal

characteristic: cell type

text: post larvae

text: Penaeus monodon

text: NotI

text: SalI

text: WSSV infected Penaeus monodon post larvae cDNA library

text: pSPORT 1


text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Hepatopancreas, muscle and haemolymph

text: Penaeus monodon

text: Hepatopancreas, muscle and haemolymph

text: WSSV infected Penaeus monodon cDNA library, Hepatopancreas, muscle and haemolymph

text: pSPORT 1


text: live

text: Generic

text: Hepatopancreas, Gills, muscle and haemolymph

text: adult

text: Penaeus monodon

characteristic: organism

text: NotI

text: SalI

text: WSSV infected Penaeus monodon cDNA library

text: pSPORT 1


text: New York University Center for Genomics and Systems Biology

text: live

text: Generic

text: Total RNA from C. elegans mixed developmental stages was prepared and used as template for ~7,000 PCR reactions each using one transcript-specific forward primer terminating at a putative stop codon and one universal anchored poly-dT primer. The amplicons were then cloned into a Gateway-compatible vector. Each well in the resulting library contains a minipool of cloned 3'UTRs targeting the same CDS. Clones were sequenced using ABI capillary sequencing and mapped onto WormBase WS180 genome sequence using BLAT. Note: Due to probable G-U base-pairing of the nucleotide anchors on the poly-dT primer, the last base or two just before the polyA tail in many of the clones do not match the genome but contain a high proportion of T to C transitions.

text: Mixed

text: Caenorhabditis elegans

text: Hermaphrodite

characteristic: sex

text: Bristol N2

text: UTRome Version 1

characteristic: title

text: Plasmid

text: Gateway pDONR P2R-P3


text: Southwest University

text: live

text: Generic

text: stem apex days 0-5 post-bolting

text: Brassica oleracea

text: stem apex

text: Trigger-Bolting-related Genes Library of Cabbage


text: live

text: Generic

text: We used suppression subtractive hybridization (SSH) to generate four subtracted cDNA libraries for the two stages in two maize inbreds (large and small grains). See publication for more information.

text: multiple stages (10 to 40 days after pollination)

text: Zea mays

text: hermaphrodite

text: endosperm

text: Zea mays suppression subtractive hybridization (SSH) Library


text: Faculdade de Medicina de Ribeirio Preto da Universidade de Sao Paulo

text: live

text: Generic

text: T.rubrum ex vivo skin infection expressed sequences.

text: Trichophyton rubrum CBS 118892

text: Trichophyton rubrum SSH Library NMMR12

text: Plasmid

text: pGEM-T


text: live

text: Generic

text: T.rubrum expressed sequences in response to ambruticin.

text: Trichophyton rubrum CBS 118892

text: Trichophyton rubrum SSH Library NMMR13

text: Plasmid

text: pGEM-T


text: University of Florida

text: live

text: Generic

text: Strawberry Festival

characteristic: cultivar

text: The library consists of generally large inserts (avg. 1.5kb) obtained from the purification of polyA RNA and subsequent cloning using the CloneMiner kit by InVitrogen. The cDNAs were cloned using Gateway technology. The library is available for public distribution.

text: Early buds, closed flowers, open flowers and senescent flowers

text: E. coli DH5alpha

characteristic: lab-host

text: Flower

characteristic: organ

text: Fragaria x ananassa

text: BsrGI

text: BsrGI

text: Strawberry Flowers; early buds to senescent

text: Plasmid


characteristic: External Id

text: Oklahoma State University

characteristic: INSDC center name

text: live

text: Generic

text: one year old seedlings

text: Pinus echinata

text: shortleaf pine 24h-48h after topkilling library


text: Oklahoma State University

characteristic: INSDC first public

text: live

text: Generic

text: one year old seedlings

text: Pinus taeda

text: loblolly pine 6d-7d after topkilling library


text: Kobe University

characteristic: INSDC last update

text: live

text: Generic

text: 40 days after sowing

text: Lotus japonicus

text: roots

text: subtracted cDNA library derived from Lotus japonicus roots at 10 days after Orobanche aegyptiaca inoculation


text: Kobe University

text: live

text: Generic

text: 40 days after sowing

characteristic: dev-stage

text: Lotus japonicus

text: roots

text: subtracted cDNA library derived from Lotus japonicus roots at 6 days after Striga hermonthica inoculation


text: Liverpool School of Tropical Medicine

text: live

text: Generic

text: pupa

text: Anopheles gambiae

text: male

text: SSH library from Anopheles gambiae male pupae


text: Beijing Academy of Agriculture and Forestry Science

text: live

text: Generic

text: Brassica rapa

text: hermaphrodite

text: leaves

characteristic: tissue-type

text: Brassica rapa housekeeping gene


text: University of Nevada, Reno

text: live

text: Generic

text: adult

text: midgut and fatbody

text: Dendroctonus ponderosae

text: female and male

text: MPB (Mountain pine beetle)

text: plasmid

characteristic: vector


text: University of Nevada, Reno

text: live

characteristic: INSDC status

text: Generic

text: adult

text: midgut and fatbody

text: Dendroctonus ponderosae

text: female and male

text: DPG (Dendroctonus ponderosae guts)

text: plasmid


text: University of Pennsylvania

text: live

text: Generic

text: lymphoblastoid cell line

characteristic: cell type

characteristic: cell-line

text: Ambion 5' RACE kit primed with gene specific primer and 5' RACE adaptor, outer and inner primers according to manufacturer's protocol

characteristic: description

text: Homo sapiens

text: male

text: Homo sapiens cell line GM11994 5' RACE


text: University of Pennsylvania

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: lymphoblastoid

text: Ambion 3' RACE kit primed with gene specific primer and 3' RACE adaptor, outer and inner primers according to manufacturer's protocol

text: Homo sapiens

characteristic: organism

text: Homo sapiens lymphoblastoid cells 3' RACE


text: Faculty of Veterinary Medicine, University of Utrecht

text: live

text: Generic

text: Total RNA was isolated from uninfected and A. marginale-infected R.microplus males salivary glands using Tri reagent (Sigma-Aldrich) following the manufacturer's protocol. The cDNA synthesis was done using the Super SMART PCR cDNA synthesis kit (Clontech-Takara). The cDNA was then directly used for PCR select subtraction (Clontech-Takara) based on a technique previously described by Diatcenko at al. (Methods Enzymol 1999, 303:349-380) and SSH libraries were constructed according to manufacturer's instructions.

text: Escherichia coli

text: Rhipicephalus microplus

text: males

characteristic: sex

text: pooled salivary glands

text: Anaplasma marginale infected Rhipicepahlus(Boophilus) microplus salivary gland SSH Library

characteristic: title

text: Plasmid

text: pGEM


text: Northwest A&F University

text: live

text: Generic

text: Capsicum annuum

text: Capsicum annuum cDNA-AFLP fragment related to Phytophthora blight resistance


text: Centro de Investigacion en Alimentacion y Desarrollo, A.C.

text: live

text: Generic

text: Different maturation stages

text: Solanum elaeagnifolium

text: Fruit

text: Solanum elaeagnifolium fruit cDNA library

text: pJet1 (Fermentas)


text: Liaoning Ocean and Fisheries Science Research Institute

text: live

text: Generic

text: E. coli

characteristic: lab-host

text: muscle

characteristic: organ

text: Verasper variegatus

text: Sfi IA

text: Sfi IB

text: muscle

text: spotted halibut SMART cDNA library from muscle

text: pDNR-LIB


characteristic: External Id

characteristic: INSDC center name

text: live

text: Generic

text: E. coli

text: spleen

text: Verasper variegatus

text: Sfi IA

text: Sfi IB

text: spleen

text: spotted halibut SMART cDNA library from spleen

text: pDNR-LIB


text: Tokyo University of Marine Science and Technology

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: postlarvae

text: Panulirus japonicus

text: Panulirus japonicus postlarvae


text: National Institute of Animal Science, Rural Development Administration

text: live

text: Generic

text: Lolium arundinaceum


text: live

text: Generic

text: Suppression subtractive hybridization libraries. Expression under nitric oxide.

text: Oryza sativa

text: SSH-NO


text: Washington University School of Medicine

text: live

text: Generic

text: Fuji

characteristic: cultivar

text: Total RNA was extracted separately from whole fruit, using the method described by Gasic et al.(2004). Poly(A)+mRNA was isolated twice from total RNA, using the Oligotex Direct mRNA kit(Qiagen). mRNA was reverse transcribed into double stranded cDNA using a modified oligo18(dT) primer with an identifying tag sequence. [Tag identification when sequencing from 5'end: insert 18(A) TGCGTG]Double stranded cDNAs were size selected (more then 500bp), adaptored with EcoRI adapters at both ends and then digested with NotI. The cDNAs were then directionally cloned into EcoRI-NotI digested pBSII SK(+) phagemid vector(Stratagene). The total number of white colony forming units (cfu) was 3.6x10e6 cfu. The background of empty clones was less than 1%. Inserts ranged from 0.8 kb to 12kb, as determined by PCR.

text: 36 days after pollination

characteristic: dev-stage

text: DH10B cells

text: Malus domestica

text: Not I

text: EcoRI

text: fruit tissue

characteristic: tissue-type

text: Phagemid

text: pBluescript II SK (+)


text: live

characteristic: INSDC status

text: Generic

text: Jonagold

text: Total RNA was extracted separately from whole fruit, using the method described by Gasic et al.(2004). Poly(A)+mRNA was isolated twice from total RNA, using the Oligotex Direct mRNA kit(Qiagen). mRNA was reverse transcribed into double stranded cDNA using a modified oligo18(dT) primer with an identifying tag sequence. [Tag identification when sequencing from 5'end: insert 18(A) TGCGAG]Double stranded cDNAs were size selected (more then 500bp), adaptored with EcoRI adapters at both ends and then digested with NotI. The cDNAs were then directionally cloned into EcoRI-NotI digested pBSII SK(+) phagemid vector(Stratagene). The total number of white colony forming units (cfu) was 1.2x10e6 cfu. The background of empty clones was less than 1%. Inserts ranged from 1.0 kb to 12kb, as determined by PCR.

text: 36 days after pollination

text: DH10B cells

text: Malus domestica

text: Not I

text: EcoRI

text: fruit tissue

text: Phagemid

text: pBluescript II SK (+)

characteristic: vector


text: live

text: Generic

text: Total RNA was extracted separately from whole root using the 'pine tree' method. Poly(A)+mRNA was isolated twice from total RNA from each stage using the Oligotex Direct mRNA kit (Qiagen). mRNA was reverse transcribed into double stranded cDNA using a modified oligo18(dT) primer with an identifying tag sequence. [Tag identification when sequencing from 5' end: insert 18(A)TCGTG] Double stranded cDNAs were size selected (more than 450 bp), adaptored with EcoRI adapters at both ends and then digested with NotI. The cDNAs were then directionally cloned into EcoR1-NotI digested pBS II SK(+) phagemid vector(Stratagene). The total number of white colony forming units (cfu) was 2.5 x 10^6 cfu (colony forming units). The background of empty clones was less than 2%. Inserts ranged from 0.5kb to 3kb, as determined by PCR.

characteristic: description

text: Baloon stage

text: DH10B ampicillin resistant

text: Malus domestica

text: Not I

text: EcoRI

text: Flower

text: Phagemid (ampicillin resistant)

text: pBluescript II SK (+)


text: live

text: Generic

characteristic: NCBI submission model

text: Total RNA was extracted separately from whole root using the 'pine tree' method. Poly(A)+mRNA was isolated twice from total RNA from each stage using the Oligotex Direct mRNA kit (Qiagen). mRNA was reverse transcribed into double stranded cDNA using a modified oligo18(dT) primer with an identifying tag sequence. [Tag identification when sequencing from 5' end: insert 18(A)TGCTG] Double stranded cDNAs were size selected (more than 450 bp), adaptored with EcoRI adapters at both ends and then digested with NotI. The cDNAs were then directionally cloned into EcoR1-NotI digested pBS II SK(+) phagemid vector(Stratagene). The total number of white colony forming units (cfu) was 1.3 x 10^6 cfu (colony forming units). The background of empty clones was less than 3%. Inserts ranged from 0.5kb to 3kb, as determined by PCR.

text: Baloon stage

text: DH10B ampicillin resistant

text: Malus domestica

text: Not I

text: EcoRI

text: Flower

text: Phagemid (ampicillin resistant)

text: pBluescript II SK (+)


text: live

text: Generic

characteristic: NCBI submission package

text: Total RNA was extracted separately from whole root using the 'pine tree' method. Poly(A)+mRNA was isolated twice from total RNA from each stage using the Oligotex Direct mRNA kit (Qiagen). mRNA was reverse transcribed into double stranded cDNA using a modified oligo18(dT) primer with an identifying tag sequence. [Tag identification when sequencing from 5' end: insert 18(A)TCGTG] Double stranded cDNAs were size selected (more than 450 bp), adaptored with EcoRI adapters at both ends and then digested with NotI. The cDNAs were then directionally cloned into EcoR1-NotI digested pBS II SK(+) phagemid vector(Stratagene). The total number of white colony forming units (cfu) was 1.1 x 10^6 cfu (colony forming units). The background of empty clones was less than 2%. Inserts ranged from 0.5kb to 3kb, as determined by PCR.

text: Baloon stage

text: DH10B ampicillin resistant

text: Malus domestica

text: Not I

text: EcoRI

text: Flower

text: Phagemid (ampicillin resistant)

text: pBluescript II SK (+)


text: live

text: Generic

text: Total RNA was extracted separately from whole root using the 'pine tree' method. Poly(A)+mRNA was isolated twice from total RNA from each stage using the Oligotex Direct mRNA kit (Qiagen). mRNA was reverse transcribed into double stranded cDNA using a modified oligo18(dT) primer with an identifying tag sequence. [Tag identification when sequencing from 5' end: insert 18(A)TCGTG] Double stranded cDNAs were size selected (more than 450 bp), adaptored with EcoRI adapters at both ends and then digested with NotI. The cDNAs were then directionally cloned into EcoR1-NotI digested pBS II SK(+) phagemid vector(Stratagene). The total number of white colony forming units (cfu) was 2.2 x 10^6 cfu (colony forming units). The background of empty clones was less than 4%. Inserts ranged from 0.5kb to 3kb, as determined by PCR.

text: Baloon stage

text: DH10B ampicillin resistant

text: Malus domestica

characteristic: organism

text: Not I

text: EcoRI

text: Flower

text: Phagemid (ampicillin resistant)

text: pBluescript II SK (+)


text: live

text: Generic

text: Total RNA was extracted separately from whole root using the 'pine tree' method. Poly(A)+mRNA was isolated twice from total RNA from each stage using the Oligotex Direct mRNA kit (Qiagen). mRNA was reverse transcribed into double stranded cDNA using a modified oligo18(dT) primer with an identifying tag sequence. [Tag identification when sequencing from 5' end: insert 18(A)TCGTG] Double stranded cDNAs were size selected (more than 450 bp), adaptored with EcoRI adapters at both ends and then digested with NotI. The cDNAs were then directionally cloned into EcoR1-NotI digested pBS II SK(+) phagemid vector(Stratagene). The total number of white colony forming units (cfu) was 6.5 x 10^6 cfu (colony forming units). The background of empty clones was less than 3%. Inserts ranged from 0.5kb to 3kb, as determined by PCR.

text: Baloon stage

text: DH10B ampicillin resistant

text: Malus domestica

text: Not I

text: EcoRI

text: Flower

text: Phagemid (ampicillin resistant)

text: pBluescript II SK (+)


text: live

text: Generic

text: Royal Gala

text: Total RNA was extracted separately from bark tissue, using the method described by Gasic et al.(2004). Poly(A)+mRNA was isolated twice from total RNA, using the Oligotex Direct mRNA kit(Qiagen). mRNA was reverse transcribed into double stranded cDNA using a modified oligo18(dT) primer with an identifying tag sequence. [Tag identification when sequencing from 5'end: insert 18(A) TGCGAG]Double stranded cDNAs were size selected (more then 500bp), adaptored with EcoRI adapters at both ends and then digested with NotI. The cDNAs were then directionally cloned into EcoRI-NotI digested pBSII SK(+) phagemid vector(Stratagene). The total number of white colony forming units (cfu) was 6.0x10e6 cfu. The background of empty clones was less than 5%. Inserts ranged from 0.8 kb to 12kb, as determined by PCR.

text: plants subjected to 5 degrees C. for 24hrs

text: DH10B cells

text: Malus domestica

text: Not I

text: EcoRI

text: bark tissue

text: Phagemid

text: pBluescript II SK (+)


text: live

text: Generic

text: Royal Gala

text: Total RNA was extracted separately from leaf tissue, using the method described by Gasic et al.(2004). Poly(A)+mRNA was isolated twice from total RNA, using the Oligotex Direct mRNA kit(Qiagen). mRNA was reverse transcribed into double stranded cDNA using a modified oligo18(dT) primer with an identifying tag sequence. [Tag identification when sequencing from 5'end: insert 18(A) TGCGTG]Double stranded cDNAs were size selected (more then 500bp), adaptored with EcoRI adapters at both ends and then digested with NotI. The cDNAs were then directionally cloned into EcoRI-NotI digested pBSII SK(+) phagemid vector(Stratagene). The total number of white colony forming units (cfu) was 7.0x10e6 cfu. The background of empty clones was less than 2%. Inserts ranged from 0.8 kb to 12kb, as determined by PCR.

text: DH10B cells

text: Malus domestica

text: Not I

text: EcoRI

text: xylem tissue

text: Phagemid

text: pBluescript II SK (+)


text: live

text: Generic

text: Royal Gala

text: Total RNA was extracted separately from xylem, using the method described by Gasic et al.(2004). Poly(A)+mRNA was isolated twice from total RNA, using the Oligotex Direct mRNA kit(Qiagen). mRNA was reverse transcribed into double stranded cDNA using a modified oligo18(dT) primer with an identifying tag sequence. [Tag identification when sequencing from 5'end: insert 18(A) TGCGTG] Double stranded cDNAs were size selected (more then 500bp), adaptored with EcoRI adapters at both ends and then digested with NotI. The cDNAs were then directionally cloned into EcoRI-NotI digested pBSII SK(+) phagemid vector(Stratagene). The total number of white colony forming units (cfu) was 1.2x10e6 cfu. The background of empty clones was less than 5%. Inserts ranged from 0.8 kb to 12kb, as determined by PCR.

text: DH10B cells

text: Malus domestica

text: Not I

text: EcoRI

text: xylem tissue

text: Phagemid

text: pBluescript II SK (+)


text: live

text: Generic

text: M.111 rootstock

text: Total RNA was extracted separately from whole root using the 'pine tree' method. Poly(A)+mRNA was isolated twice from total RNA from each stage using the Oligotex Direct mRNA kit (Qiagen). mRNA was reverse transcribed into double stranded cDNA using a modified oligo18(dT) primer with an identifying tag sequence. [Tag identification when sequencing from 5' end: insert 18(A)TCGTG] Double stranded cDNAs were size selected (more than 450 bp), adaptored with EcoRI adapters at both ends and then digested with NotI. The cDNAs were then directionally cloned into EcoR1-NotI digested pBS II SK(+) phagemid vector(Stratagene). The total number of white colony forming units (cfu) was 7.4 x 10^6 cfu (colony forming units). The background of empty clones was less than 3%. Inserts ranged from 0.5kb to 3kb, as determined by PCR.

text: young root

text: DH10B ampicillin resistant

text: Malus domestica

text: Not I

text: EcoRI

text: Root

text: Phagemid (ampicillin resistant)

text: pBluescript II SK (+)


text: live

text: Generic

text: Geneva 3041

text: Total RNA was extracted separately from whole root using the 'pine tree' method. Poly(A)+mRNA was isolated twice from total RNA from each stage using the Oligotex Direct mRNA kit (Qiagen). mRNA was reverse transcribed into double stranded cDNA using a modified oligo18(dT) primer. Double stranded cDNAs were size selected (more than 450 bp), adaptored with EcoRI adapters at both ends and then digested with NotI. The cDNAs were then directionally cloned into EcoR1-NotI digested pBS II SK(+) phagemid vector(Stratagene). The total number of white colony forming units (cfu) was 1.5 x 10^6 cfu cfu (colony forming units). The background of empty clones was less than 6%. Inserts ranged from 0.5kb to 4 kb, as determined by PCR.

text: young root

text: DH10B ampicillin resistant

text: Malus hybrid rootstock

text: Not I

text: EcoRI

text: Root

text: Phagemid (ampicillin resistant)

text: pBluescript II SK (+)


text: live

text: Generic

text: Total RNA was extracted separately from whole root using the 'pine tree' method. Poly(A)+mRNA was isolated twice from total RNA from each stage using the Oligotex Direct mRNA kit (Qiagen). mRNA was reverse transcribed into double stranded cDNA using a modified oligo18(dT) primer with an identifying tag sequence. [Tag identification when sequencing from 5' end: insert 18(A)TCGTG] Double stranded cDNAs were size selected (more than 450 bp), adaptored with EcoRI adapters at both ends and then digested with NotI. The cDNAs were then directionally cloned into EcoR1-NotI digested pBS II SK(+) phagemid vector(Stratagene). The total number of white colony forming units (cfu) was 1 x 10^6 cfu (colony 1%. Inserts ranged from 0.5kb to 3kb, as determined by PCR.

text: actively growing shoot

text: DH10B ampicillin resistant

text: Malus domestica

text: Not I

text: EcoRI

text: Shoot

text: Phagemid (ampicillin resistant)

text: pBluescript II SK (+)


text: live

text: Generic

text: Royal Gala

text: Total RNA was extracted separately from bark, using the method described by Gasic et al.(2004). Poly(A)+mRNA was isolated twice from total RNA, using the Oligotex Direct mRNA kit(Qiagen). MRNA was reverse transcribed into double stranded cDNA using a modified oligo18(dT) primer with an identifying tag sequence. [Tag identification when sequencing from 5'end: insert 18(A) TGCGAG] Double stranded cDNAs were size selected (more then 500bp), adaptored with EcoRI adapters at both ends and then digested with NotI. The cDNAs were then directionally cloned into EcoRI-NotI digested pBSII SK(+) phagemid vector(Stratagene). The total number of white colony forming units (cfu) was 4.5x10e6 cfu. The background of empty clones was less than 3%. Inserts ranged from 0.8 kb to 12kb, as determined by PCR.

text: plants subjected to water stress for 1week at the 45% pot/plant mass

text: DH10B cells

text: Malus domestica

text: Not I

text: EcoRI

text: bark tissue

text: Phagemid

text: pBluescript II SK (+)


text: Washington University School of Medicine

text: live

text: Generic

text: Royal Gala

text: Total RNA was extracted separately from leaf tissue, using the method described by Gasic et al.(2004). Poly(A)+mRNA was isolated twice from total RNA, using the Oligotex Direct mRNA kit(Qiagen). mRNA was reverse transcribed into double stranded cDNA using a modified oligo18(dT) primer with an identifying tag sequence. [Tag identification when sequencing from 5'end: insert 18(A) TCGGTG]Double stranded cDNAs were size selected (more then 500bp), adaptored with EcoRI adapters at both ends and then digested with NotI. The cDNAs were then directionally cloned into EcoRI-NotI digested pBSII SK(+) phagemid vector(Stratagene). The total number of white colony forming units (cfu) was 4.0x10e6 cfu. The background of empty clones was less than 2%. Inserts ranged from 0.8 kb to 12kb, as determined by PCR.

text: DH10B cells

text: Malus domestica

text: Not I

text: EcoRI

text: leaf tissue

text: Phagemid

text: pBluescript II SK (+)


text: live

text: Generic

text: Royal Gala

text: Total RNA was extracted separately from root tissue, using the method described by Gasic et al.(2004). Poly(A)+mRNA was isolated twice from total RNA, using the Oligotex Direct mRNA kit(Qiagen). mRNA was reverse transcribed into double stranded cDNA using a modified oligo18(dT) primer with an identifying tag sequence. [Tag identification when sequencing from 5'end: insert 18(A) TGCGTG]Double stranded cDNAs were size selected (more then 500bp), adaptored with EcoRI adapters at both ends and then digested with NotI. The cDNAs were then directionally cloned into EcoRI-NotI digested pBSII SK(+) phagemid vector(Stratagene). The total number of white colony forming units (cfu) was 6.4x10e6 cfu. The background of empty clones was less than 3%. Inserts ranged from 0.8 kb to 12kb, as determined by PCR.

text: plants subjected to water stress for 1 week at the 45% pot/plant mass

text: DH10B cells

text: Malus domestica

text: Not I

text: EcoRI

text: root tissue

text: Phagemid

text: pBluescript II SK (+)


text: University of Wisconsin-Milwaukee

text: live

text: Generic

text: Library constructed from RNA obtained from the brains of juvenile yellow perch (equal males and females).

text: juvenile

text: Perca flavescens

text: pooled brains

text: Yellow perch control brain library

text: pBluescript SK-


text: University of Wisconsin-Milwaukee

text: live

text: Generic

text: Library constructed from RNA obtained from the brains of juvenile yellow perch (equal males and females) treated for 9 weeks with 15ug estradiol-17B/gm diet.

text: juvenile treated for 9 weeks with 15ug estradiol

text: Perca flavescens

text: pooled brains

text: Yellow perch estrogen-stimulated brain library

text: pBluescript SK-


text: Embrapa Dairy Cattle Research Center and Federal University of Vicosa

text: live

text: Generic

text: Epithelium

characteristic: cell type

text: These EST sequences were obtained as part of a collaborative project between Embrapa Dairy Cattle Research Center and the Federal University of Vicosa (Brazil). Skin samples of F2 population were collected on day 5 and 12 after artificial infestation with Boophilus microplus. RNA samples were extracted with Maxi Kit (Qiagen) and the cDNA libraries were constructed with Superscript Plasmid System with Gateway Technology for cDNA Synthesis and Cloning (Invitrogen).

text: Adult

text: E.coli

text: Bos indicus x Bos taurus

text: NotI

text: SalI

text: both

characteristic: sex

text: Breed: Holstein x Gyr (hybrid cattle)

characteristic: strain

text: Skin

text: EMBRAPA-UFV Brazilian F2 (Holstein x Gyr) Cattle Skin cDNA Library

text: ampicilin resistant


text: Graduate School, College of Life Science, CHA University

text: live

text: Generic

text: fibroblast-like

text: Homo sapiens

text: male

text: testis

text: human spermatogonial stem cell-like cells cDNA Library

characteristic: title


characteristic: External Id

text: INRA - SCRIBE

text: live

text: Generic

characteristic: lab-host

text: Oncorhynchus mykiss

text: Rainbow trout multi-tissues library (resequencing) (tccn)


text: College of Life Science and Technology, Southwest University for Nationalities

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: Leymus secalinus

text: leaves

text: cDNA subtractive library from leaves of Leymus secalinus by drought stress

text: Plasmid


text: University of Saskatchewan, Crop Development Centre

characteristic: INSDC last update

text: live

text: Generic

text: CDC Dancer

characteristic: cultivar

text: watery

text: Avena sativa

text: seed


text: live

text: Generic

text: CDC Dancer

text: early milk

characteristic: dev-stage

text: Avena sativa

text: seed

characteristic: tissue-type


text: live

characteristic: INSDC status

text: Generic

text: CDC Dancer

text: late milk/early dough

text: Avena sativa

text: seed


text: National Institute of Allergy and Infectious Diseases

text: live

text: Generic

text: Secretory

text: PCR-based cDNA library made with SMART cDNA library construction kit from Clontech

characteristic: description

text: Adult

text: Salivary gland

characteristic: organ

text: Anopheles freeborni

text: Female

text: Salivary gland

text: Adult female Anopheles freeborni salivary glands AFree1

text: Phage

text: Lambda TriplEx2

characteristic: vector


text: Inner Mongolia AgricuLtural University

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: The total RNA was prepared with Trizol reagent from secondary follicles at growth phase and telegon, and the SSH cDNA library was constructed by PCR-Select cDNA Subtraction Kit of CLONTECH (Cat.No.637401).

text: secondary hair follicle

text: Capra hircus

characteristic: organism

text: Rsa I

text: secondary hair follicle

text: SSH cDNA library of secondary hair follicle of cashmere goat

text: Plasmid

text: pGEM-T


text: EMBL Heidelberg

text: live

text: Generic

text: Sequencing was performed at Genoscope. This sequence is from a cDNA library generated by Kristin Tessmar-Raible and Heidi Snyman. cDNA was prepared from poly-A RNA extracted from four batches ofPlatynereis dumerilii larvae at 48hpf. A poly-T NotI primer adapter was used for the first reverse transcriptase reaction. SalI adapters were added, then fragments were NotI digested and directionally cloned into the SalI/NotI restriction site of the pCMVSport6 vector (Invitrogen).

text: 48 hour post fertilization larva

text: Platynereis dumerilii

text: SalI

text: NotI

text: Platynereis dumerilii 48 hour post fertilization library


text: The University of Queensland

text: live

text: Generic

text: 75% Epiboly

text: Danio rerio

text: male

characteristic: sex

text: AB strain

characteristic: strain

text: whole embryo

text: Zebrafish embryo 75% Epiboly stage library


characteristic: External Id

text: Beijing Forestry University

text: live

text: Generic

text: Ophiopogon japonicus

text: leaf

text: ethophon treated Ophiopogon japonicus ( L. f . ) KerGawl cDNA library

characteristic: title


text: NRC Plant Biotechnology Institute

characteristic: INSDC center name

text: live

text: Generic

text: The library NBERO1CH was constructed from mRNA isolated from the roots of hydroponically-grown Nicotiana benthamiana plants treated with methyl jasmonate using a cDNA library synthesis kit (Stratagene). cDNAs ligated into the plasmid vector pBluescript SK+.

text: Root

text: Nicotiana benthamiana

text: XhoI

text: Plasmid

text: pBluescript SK+


text: NRC Plant Biotechnology Institute

characteristic: INSDC first public

text: live

text: Generic

text: The library NBLEL3 was constructed using the suppression subtractive hybridization technique (Clontech PCR-select subtractive cDNA library kit) with RNA isolated from hydroponically-grown Nicotiana benthamiana plants as previously described (Liu & Page (2008) Plant Methods 4: 5). NBLEL3 was created using mRNA from methyl jasmonate-treated roots as tester and untreated leaves as driver. The subtracted cDNAs were amplified using primers containing BamH1 and EcoR1 sites, cloned directly into the TRV-RNA2 plasmid pYL156 (AF406991) and electroporated into Agrobacterium tumefaciens. cDNA inserts amplified from A. tumefaciens cultures were used as sequencing templates.

text: Root

text: Nicotiana benthamiana

text: Plasmid


text: NRC Plant Biotechnology Institute

characteristic: INSDC last update

text: live

text: Generic

text: The library NBREL2 was constructed using the suppression subtractive hybridization technique (Clontech PCR-select subtractive cDNA library kit) with RNA isolated from hydroponically-grown Nicotiana benthamiana plants as previously described (Liu & Page (2008) Plant Methods 4: 5). NBREL2 was created using mRNA from methyl jasmonate-treated roots as tester and untreated roots as driver. The subtracted cDNAs were amplified using primers containing BamH1 and EcoR1 sites, cloned directly into the TRV-RNA2 plasmid pYL156 (AF406991) and electroporated into Agrobacterium tumefaciens. cDNA inserts amplified from A. tumefaciens cultures were used as sequencing templates.

text: Root

text: Nicotiana benthamiana

text: Plasmid


text: NRC Plant Biotechnology Institute

text: live

text: Generic

text: The library NBREL4 was constructed using the suppression subtractive hybridization technique (Clontech PCR-select subtractive cDNA library kit) with RNA isolated from hydroponically-grown Nicotiana benthamiana plants as previously described (Liu & Page (2008) Plant Methods 4: 5). NBREL4 was created using mRNA from methyl jasmonate-treated roots as both tester and driver. The subtracted cDNAs were amplified using primers containing BamH1 and EcoR1 sites, cloned directly into the TRV-RNA2 plasmid pYL156 (AF406991) and electroporated into Agrobacterium tumefaciens. cDNA inserts amplified from A. tumefaciens cultures were used as sequencing templates.

text: Root

text: Nicotiana benthamiana

text: Plasmid


text: live

text: Generic

text: root and petal cDNA library

text: Petunia axillaris subsp. axillaris

text: pool of root and petal tissue

text: Petunia axillaris subsp. axillaris pool of root and petal tissue


text: National Research Council

text: live

text: Generic

text: Homo sapiens

text: breast carcinoma

text: sstDNA 1360


text: Chiba University

text: live

text: Generic

text: Glycyrrhiza uralensis

text: rhizome

text: TPC


text: Chiba University

text: live

text: Generic

text: Glycyrrhiza uralensis

text: rhizome

text: RPSC


text: Chiba University

text: live

text: Generic

text: Glycyrrhiza uralensis

text: rhizome

text: MEGA


text: Department of Biotechnology and Bioengineering, Dongeui University

text: live

text: Generic

text: conidial germination

text: Colletotrichum acutatum

text: Colletotrichum acutatum conidial germination stage Library


text: Virginia State University

text: live

text: Generic

text: A cDNA constructed using Clontech's Creator Smart cDNA construction kit. Agencourt sequencing services were used for sequencing the clones generated at VSU.

text: mature embryo

characteristic: lab-host

text: Vernonia galamensis

text: embryo

text: Vernonia Galamensis L embryo cDNA library


text: National Institute of Livestock and Grassland Science

text: live

text: Generic

text: apomixis strain

text: heading time

characteristic: dev-stage

text: Megathyrsus maximus

text: immature panicle, pistil

text: Ku5954 apomictic immature panicle cDNA library


text: live

text: Generic

text: Apomictic progeny GM64-3A was obtained by crossing between a sexual cultivar Noh PL 1 and apomixis cultivar Natsukaze.

text: heading time

text: Megathyrsus maximus

text: immature panicle, pistil

characteristic: tissue-type

text: GM64-3A apomictic immature panicle cDNA library


text: Kyoto University

text: live

characteristic: INSDC status

text: Generic

text: adult

text: Cynops pyrrhogaster

text: lens regenerating iris


text: Kyoto University

text: live

text: Generic

text: adult

text: Cynops pyrrhogaster

text: ovary


text: Kyoto University

text: live

text: Generic

text: adult

text: Cynops pyrrhogaster

text: intact limb


text: College of Natural Resource and Environment, South China Agricultural University

text: live

text: Generic

text: adult

text: Bactrocera dorsalis

text: male and female

text: antenna

text: T7 phage display cDNA library of antenna from Bactrocera dorsalis Hendel


text: Nihon University

text: live

text: Generic

text: tadpole

text: Rana pirica

text: head body

text: Rana pirica tadpole head body


text: University of Victoria

text: live

text: Generic

text: Tissue contributors: B. Campbell et al - Sable Fish Canada

characteristic: description

text: brain, kidney, gill

text: Anoplopoma fimbria

text: brain, kidney, gill

text: afimevh mixed_tissue Anoplopoma fimbria cDNA


text: Fisheries College/Fisheries Biotechnology Institute, Jimei University

text: live

text: Generic

text: Larimichthys crocea

text: male and female

text: Gonad

text: Large yellow croaker gonad normalized cDNA Library

text: Plasmid

text: pDNR-LIB


text: University of Neuchatel

text: live

text: Generic

text: Chasselas

characteristic: cultivar

text: cDNA SSH library of leaves from two-month-old Vitis vinifera seedlings cultivar 'Chasselas' (susceptible) infected with Plasmopara viticola. The cDNA SSH library was carried out using the PCR-Select-Hybridization kit according to the manufacturer instructions(Clontech). The resulting cDNA were cloned into pGEM-T easy vector (Promega).

text: two-month-old seedlings

text: Vitis vinifera

text: Leaf

text: Plasmid

text: pGEM-T easy


text: Indian Institute of Vegetable Research

text: live

text: Generic

text: After drought treatment leave tissues were collected for cDNA preparation, cDNA was blunted and cloned in pUC19 clonning vector, sequencing was done with ABI 3130 Automated sequencer.

text: E.coli

text: Solanum lycopersicum

text: Leaf

text: Drought Stressed Tomato leaves

text: Plasmid

characteristic: vector


text: MPI for Molecular Genetics

text: live

text: Generic

characteristic: NCBI submission model

text: adult individuals were grown from cultured eggs from wild populations from Austria ; country: Austria:Danubia delta ; collection_date: 2006 ; collected_by: Erich Eder. Total RNA was isolated from whole organism using Trizol (Invitrogen) and purified including a DNase digest. The obtained template was used for cDNA synthesis (CreatorSmart, Clontech). The cDNA was ligated in pDNRlib vector (Clontech) and transformed in E. coli strain DH10B (Invitrogen). Sequences were obtained from the 5 prime end with BigDye chemistry (ABI). Library contruction and sequencing were performed at the MPI for Molecular Genetics Berlin.

text: adult

text: E. coli strain DH10B

text: Triops cancriformis

text: complete specimen, parts of digestive tract were excluded

text: pDNRlib


text: Huazhong Agricultural University

text: live

text: Generic

characteristic: NCBI submission package

text: Suppression subtractive hybridization (SSH) library. Defense-related mRNA.

text: Boehmeria clidemioides var. diffusa

text: Defense-related mRNA induced by ramie anthracnose


text: University of Stavanger

text: live

text: Generic

text: Suzanne

text: 3 week old plant

text: Solanum lycopersicum

characteristic: organism

text: Leaf

text: COREL


text: University of Victoria

text: live

text: Generic

text: Tissue contributors: S. Jones (Fisheries and Oceans Canada)

text: whole body

characteristic: organ

text: Lepeophtheirus salmonis

text: Pacific

text: mixed tissue, mixed stage

text: lsalevj mixed_tissue_mixed_stage Lepeophtheirus salmonis (Pacific) cDNA


text: University of Victoria

text: live

text: Generic

text: Tissue contributors: SRM Jones (Fisheries and Oceans; PBS, Nanaimo BC)

text: whole

text: Lepeophtheirus salmonis

text: female

characteristic: sex

text: Pacific

characteristic: strain

text: whole

text: lsalaf whole Lepeophtheirus salmonis (Pacific) cDNA

text: pBlueScriptIISK+


characteristic: External Id

text: University of Victoria

text: live

text: Generic

text: whole

text: Lepeophtheirus salmonis

text: male

text: Pacific

text: whole

text: lsalam whole Lepeophtheirus salmonis (Pacific) cDNA

characteristic: title

text: pBlueScriptIISK+


text: University of Victoria

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: whole

text: Lepeophtheirus salmonis

text: Pacific

text: whole

text: lsalch1 whole Lepeophtheirus salmonis (Pacific) cDNA

text: pBlueScriptIISK+


text: University of Victoria

characteristic: INSDC last update

text: live

text: Generic

text: whole

text: Lepeophtheirus salmonis

text: Pacific

text: whole

text: lsalch3 whole Lepeophtheirus salmonis (Pacific) cDNA

text: pBlueScriptIISK+


text: University of Victoria

text: live

text: Generic

text: whole

text: Lepeophtheirus salmonis

text: Pacific

text: whole

text: lsalch4 whole Lepeophtheirus salmonis (Pacific) cDNA

text: pBlueScriptIISK+


text: University of Victoria

text: live

text: Generic

text: whole

text: Lepeophtheirus salmonis

text: Pacific

text: whole

text: lsalcop whole Lepeophtheirus salmonis (Pacific) cDNA

text: pBlueScriptIISK+


text: University of Victoria

text: live

text: Generic

text: whole

text: Lepeophtheirus salmonis

text: female

text: Pacific

text: whole

text: lsalpf whole Lepeophtheirus salmonis (Pacific) cDNA

text: pBlueScriptIISK+


text: University of Victoria

text: live

text: Generic

text: whole

text: Lepeophtheirus salmonis

text: male

text: Pacific

text: whole

text: lsalpm whole Lepeophtheirus salmonis (Pacific) cDNA

text: pBlueScriptIISK+


text: University of Wisconsin-Milwaukee

text: live

text: Generic

text: Library constructed from RNA obtained from follicle-enriched ovarian tissue from female yellow perch injected in vivo with human chorionic gonadotropin.

text: Perca flavescens

text: follicle-enriched ovarian tissue

text: Yellow perch ovarian library 1

text: pBK-CMV


text: University of Wisconsin-Milwaukee

text: live

text: Generic

text: Library constructed from RNA obtained from follicle-enriched ovarian tissue from in vitro incubations of yellow perch follicles stimulated with 17a, 20b-dihydroxy-4-pregnen-3-one for 32 hours.

text: Perca flavescens

text: follicle-enriched ovarian tissue

text: Yellow perch ovarian library 2

text: pBK-CMV


text: University of Wisconsin-Milwaukee

text: live

text: Generic

text: Library constructed from RNA obtained from follicle-enriched ovarian tissue from in vitro incubations of yellow perch follicles stimulated with 17a, 20b-dihydroxy-4-pregnen-3-one for 6 and 21 hours (combined).

text: Perca flavescens

text: follicle-enriched ovarian tissue

text: Yellow perch ovarian library 3

text: pBK-CMV


text: University of Wisconsin-Milwaukee

text: live

text: Generic

text: Library constructed from RNA obtained from follicle-enriched ovarian tissue from in vitro incubations of yellow perch follicles stimulated with 17a, 20b-dihydroxy-4-pregnen-3-one for 24 hours.

text: Perca flavescens

text: follicle-enriched ovarian tissue

text: Yellow perch ovarian library 4

text: pBK-CMV


text: Bose Institute

text: live

text: Generic

text: cDNA-AFLP analysis

text: Cicer arietinum

text: roots

text: Chickpea cv. JG62 infected by Fusarium oxysporum f. sp. ciceri (Race 1)


text: Bose Institute

text: live

text: Generic

text: cDNA-AFLP analysis

text: Cicer arietinum

text: roots

text: Chickpea cv. WR315 infected by Fusarium oxysporum f. sp. ciceri (Race 1)


text: USDA-ARS Western Regional Research Center

text: live

text: Generic

text: Total RNA was used for ds cDNA synthesis using Clontech SMART protocol (Zhu et. al. (2001) Biotechniques 30: 892-897). SMART prepared amplified cDNA was then normalized using DSN normalization method (Zhulidov et. al., (2004) Nucleic Acid Res. 32(3): e37. Normalization included cDNA denaturation/reassociation, treatment of duplex specific nuclease (DSN) (Shagin et. al. (2002) Genome Res. 12:1935-1942) and amplification of normalized fraction by PCR. Resulted normalized cDNA was digested with SfiI and subcloned into pGADT7Rec-AB. Quality Read Lengths from sequences were extracted with Phred program. File trimming based on sequence mark-up done with cross_match program, minor vector/adaptor sequences remained.

text: 1.2 to 3 month

characteristic: dev-stage

text: Taraxacum kok-saghyz

text: SfiIA

text: SfiIB

text: root

text: Taraxacum kok-saghyz root cDNA library (TKN)

text: Plasmid


text: live

text: Generic

text: Quality Read Lengths from sequences were extracted with Phred program. File trimming based on sequence mark-up done with cross_match program, minor vector/adaptor sequences remained.

text: 1.2 to 3 month

text: Taraxacum kok-saghyz

text: root

characteristic: tissue-type

text: Taraxacum kok-saghyz root cDNA library (TKR)


text: CSIRO Plant Industry

text: live

characteristic: INSDC status

text: Generic

text: Aposporous Initial Cell

characteristic: cell type

text: C4D NZ

text: Aposporous Initial Cells harvested by Laser Capture Microdissection were used for RNA isolation and cDNA library construction.

text: female gametophyte developmental stage 4

text: Pilosella caespitosa

text: XhoI

text: EcoRI

text: Ovule

text: Hieracium caespitosum aposporous initial cell cDNA library

text: Plasmid

text: pBluescript SK(-)


text: AgResearch Limited

text: live

text: Generic

text: Single animal. EST library not normalised.

characteristic: description

text: Adult (6 years old)

text: sheep 5, Auckland Medical School

characteristic: isolate

text: Skin (flank)

text: Ovis aries

text: female

text: New Zealand Romney Marsh bred

text: OASA

text: Plasmid

text: pBK-CMV


text: AgResearch Limited

text: live

text: Generic

characteristic: NCBI submission model

text: Single animal. EST library not normalised. Singleton pregnancy. Fetal sheep at 130 days since conception.

text: Fetal

text: sheep 6, Auckland Medical School

text: Brain

text: Ovis aries

text: New Zealand Romney Marsh bred

text: OFBB

text: Plasmid

text: pBK-CMV

characteristic: vector


text: AgResearch Limited

text: live

text: Generic

characteristic: NCBI submission package

text: Fetal

text: sheep 6, Auckland Medical School

text: Skin (flank)

text: Ovis aries

characteristic: organism

text: New Zealand Romney Marsh bred

text: OFSA

text: Plasmid

text: pBK-CMV


text: AgResearch Limited

text: live

text: Generic

text: Single animal. EST library not normalised. Natural facial eczema infection. No GGT levels measure at slaughter. No clinical records. Spore counts reached 30,000-60,000, if 30,000 or more zinc was administered.

text: Adult (6 years old)

text: sheep 1, Uni. farm, Totara Rd, Ramarama

text: Sub-mandible lymph nodes from a sheep with moderate facial eczema

characteristic: organ

text: Ovis aries

text: female

characteristic: sex

text: New Zealand Romney Marsh bred

text: OLBA

text: Plasmid

text: pCMV-Script


characteristic: External Id

text: AgResearch Limited

text: live

text: Generic

text: Adult (6 years old)

text: sheep 2, Uni. farm, Totara Rd, Ramarama

text: Hepatic lymph nodes from a sheep with mild facial eczema

text: Ovis aries

text: female

text: New Zealand Romney Marsh bred

characteristic: strain

text: OLCA

text: Plasmid

text: pCMV-Script


text: AgResearch Limited

characteristic: INSDC center name

text: live

text: Generic

text: Adult (6 years old)

text: sheep 3, Uni. farm, Totara Rd, Ramarama

text: Hepatic lymph nodes from a sheep with old facial eczema

text: Ovis aries

text: female

text: New Zealand Romney Marsh bred

text: OLDA

characteristic: title

text: Plasmid

text: pCMV-Script


text: AgResearch Limited

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Adult (6 years old)

text: sheep 1, Uni. farm, Totara Rd, Ramarama

text: Paratoid lymph nodes from a sheep with moderate facial eczema

text: Ovis aries

text: female

text: New Zealand Romney Marsh bred

text: OLEF

text: Plasmid

text: pCMV-Script


text: AgResearch Limited

text: live

text: Generic

text: Adult (6 years old)

characteristic: dev-stage

text: sheep 4, Auckland Medical School

text: Ovis aries

text: female

text: New Zealand Romney Marsh bred

text: OLFA

text: Plasmid

text: pCMV-Script


text: AgResearch Limited

text: live

characteristic: INSDC status

text: Generic

text: Adult (6 years old)

text: sheep 4, Auckland Medical School

text: Ovis aries

text: female

text: New Zealand Romney Marsh bred

text: OLFB

text: Plasmid

text: pCMV-Script


text: AgResearch Limited

text: live

text: Generic

text: Adult (6 years old)

text: sheep 5, Auckland Medical School

text: Mesenteric lymph node

text: Ovis aries

text: female

text: New Zealand Romney Marsh bred

text: OMNC

text: Plasmid

text: pBK-CMV


text: AgResearch Limited

text: live

text: Generic

text: Adult (6 years old)

text: sheep 5, Auckland Medical School

text: Tonsil

text: Ovis aries

text: female

text: New Zealand Romney Marsh bred

text: OTNA

text: Plasmid

text: pBK-CMV


text: AgResearch Limited

text: live

text: Generic

text: Adult (6 years old)

text: sheep 5, Auckland Medical School

text: Thyroid

text: Ovis aries

text: female

text: New Zealand Romney Marsh bred

text: OTYA

text: Plasmid

text: pBK-CMV


text: AgResearch Limited

text: live

text: Generic

text: Adult (6 years old)

text: sheep 1, Uni. farm, Totara Rd, Ramarama

text: Ovis aries

text: female

text: New Zealand Romney Marsh bred

text: OLEA (sheep 1)

text: Plasmid

text: pCMV-Script


text: AgResearch Limited

text: live

text: Generic

text: Adult (6 years old)

text: sheep 1, Uni. farm, Totara Rd, Ramarama

text: Ovis aries

text: female

text: New Zealand Romney Marsh bred

text: OLEB (sheep 1)

text: Plasmid

text: pCMV-Script


text: Auburn University

text: live

text: Generic

text: Owari

characteristic: cultivar

text: cDNA-AFLP

text: Citrus unshiu

text: Citrus unshiu cold stress with cDNA-AFLP


text: Institute of Hydrobiology, CAS

text: live

text: Generic

text: Suppression Subtractive Hybridization

characteristic: description

text: Phaeodactylum tricornutum

text: from the Institute of Oceanology, CAS

text: Phaeodactylum tricornutum nitrogen starvation subtractive Library


text: National Institute of Oceanography

text: live

text: Generic

text: Suppression subtractive hybridization was carried out. The products were then cloned into pGEMTeasy vector.

text: 15 day postlarvae

text: Penaeus indicus

text: whole animal

characteristic: tissue-type

text: Vibrio harveyi challenged Penaeus indicus postlarvae cDNA library

text: pGEMTeasy


text: National Institute of Biomedical Innovation

text: live

text: Generic

characteristic: NCBI submission model

text: 1st strand cDNA was primed with an oligo(dT) primer [ATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded using specific 5' and 3' primers and amplified by PCR. The PCR cDNA was synthesized product was digested with SfiI and size selection was performed to exclude fragments <1.5kb. The SfiI-digested PCR product was cloned into distinct DraIII sites of pME18S-FL3. XhoI sites just outside the DraIII sites can be used to isolate the cDNA insert. Libraries were constructed by oligo-capping method. Cutoff criteria for the quality value (QV) was 15.

text: 3 years old

characteristic: lab-host

text: Macaca fascicularis

text: DraIII (CACTGTGTG)

text: DraIII (CACTGTGTG)

text: male

text: pancreas

text: macaque pancreas cDNA library QpaA

text: pME18S-FL3 (Acc.No. AB009864)


text: DOE Joint Genome Institute

text: live

text: Generic

characteristic: NCBI submission package

text: The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected >2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed and sequencing performed at the DOE Joint Genome Institute (Walnut Creek, CA).

text: Guillardia theta

text: SalI

text: NotI

text: CCMP2712 (single-cell isolate of CCMP327)

text: CCFI 777453.Guillardia.theta.2712.cDNA (H)

characteristic: vector


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 0.6-2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed and sequencing performed at the DOE Joint Genome Institute (Walnut Creek, CA).

text: Guillardia theta

characteristic: organism

text: SalI

text: NotI

text: CCFN 777453.Guillardia.theta.2712.cDNA (L)


text: Universidade Federal de Sao Carlos

text: live

text: Generic

text: The mRNA of the venom gland were purified with PolyATtract mRNA Isolation Systems (Promega) and library constructed using CloneMiner cDNA Library Construction Kit (Invitrogen).

text: Adult

text: E.coli

text: Venom gland

characteristic: organ

text: Bothrops alternatus

text: BsrG I

text: BsrG I

text: Male

characteristic: sex

text: Bothrops alternatus venom gland transcriptome

text: Plasmid

text: pDORN222 (Invitrogen)


characteristic: External Id

text: University of Kentucky

text: live

text: Generic

text: From Lolium pratense inflorescence with fungal stroma. Prepared using CloneTech SMART cDNA cloning system: Zhu YY, Machleder EM, Chenchik A, Li R, and Siebert PD. 2001. Reverse transcriptase template switching: A SMART (TM) approach for full-length cDNA library construction. Biotechniques 30: 892-897.

text: Stroma

text: Epichloe festucae

text: SfiI

text: SfiI

text: hermaphrodite

text: Phagemid

text: lambda TriplEX2


text: University of Kentucky

characteristic: INSDC center name

text: live

text: Generic

text: Prepared using CloneTech SMART cDNA cloning system: Zhu YY, Machleder EM, Chenchik A, Li R, and Siebert PD. 2001. Reverse transcriptase template switching: A SMART (TM) approach for full-length cDNA library construction. Biotechniques 30: 892-897.

text: Inflorescence with fungal stroma

text: Festuca pratensis

text: SfiI

text: SfiI

text: hermaphrodite

text: Phagemid

text: lambda TriplEX2


text: University of Kentucky

text: live

text: Generic

text: From pre-anthesis Lolium pratense inflorescence. Prepared using CloneTech SMART cDNA cloning system: Zhu YY, Machleder EM, Chenchik A, Li R, and Siebert PD. 2001. Reverse transcriptase template switching: A SMART (TM) approach for full-length cDNA library construction. Biotechniques 30: 892-897.

text: Epichloe festucae

text: SfiI

text: SfiI

text: hermaphrodite

characteristic: title

text: Phagemid

text: lambda TriplEX2


text: University of Kentucky

characteristic: INSDC first public

text: live

text: Generic

text: Inflorescence (pre-anthesis)

text: Festuca pratensis

text: SfiI

text: SfiI

text: hermaphrodite

text: Phagemid

text: lambda TriplEX2


text: University of Kentucky

characteristic: INSDC last update

text: live

text: Generic

text: From Lolium pratense inflorescence. Prepared using CloneTech SMART cDNA cloning system: Zhu YY, Machleder EM, Chenchik A, Li R, and Siebert PD. 2001. Reverse transcriptase template switching: A SMART (TM) approach for full-length cDNA library construction. Biotechniques 30: 892-897.

text: Epichloe festucae

text: SfiI

text: SfiI

text: hermaphrodite

text: Phagemid

text: lambda TriplEX2


text: University of Kentucky

text: live

text: Generic

text: Inflorescence

text: Festuca pratensis

text: SfiI

text: SfiI

text: hermaphrodite

text: Phagemid

text: lambda TriplEX2


text: University of Kentucky

text: live

text: Generic

text: From Lolium pratense pseudostem. Prepared using CloneTech SMART cDNA cloning system: Zhu YY, Machleder EM, Chenchik A, Li R, and Siebert PD. 2001. Reverse transcriptase template switching: A SMART (TM) approach for full-length cDNA library construction. Biotechniques 30: 892-897.

text: Epichloe festucae

text: SfiI

text: SfiI

text: hermaphrodite

text: Phagemid

text: lambda TriplEX2


text: University of Kentucky

text: live

text: Generic

text: Pseudostem

text: Festuca pratensis

text: SfiI

text: SfiI

text: hermaphrodite

text: Phagemid

text: lambda TriplEX2


text: Peking University

text: live

text: Generic

text: stamen at stage 5 and 6

characteristic: dev-stage

text: stamen

text: Cucumis sativus

text: cucumber stamen suppression subtractive hybridization


text: Marine Science College of Zhejiang Ocean University

text: live

characteristic: INSDC status

text: Generic

text: adult

text: E. coli

text: Haemolymph

text: Mytilus coruscus

text: Male and Female

text: Haemolymph

text: mco-hae

text: pCMV sport6


text: live

text: Generic

text: adult

text: E. coli

text: foot-gland

text: Mytilus coruscus

text: Male and Female

text: foot-gland

text: mco-foot

text: pCMV sport6


text: McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University,

text: live

text: Generic

text: cDNA was synthesized from total RNA isolated and fragments amplified by PCR

text: Adult

text: Midgut

text: Anopheles gambiae

text: Female

text: Keele

characteristic: strain

text: Midgut

text: Anopheles gambiae Adult female midgut


text: National University of Ireland Galway (NUIG)

text: live

text: Generic

text: E. coli Top 10

text: Sparus aurata

text: liver

text: Aquafirst Sea Bream SSH selection confinement liver early (gsgl01)

text: PCR 2.1


text: Universita di Pisa

text: live

text: Generic

text: Villafranca

characteristic: cultivar

text: 2 year old plant

text: Populus alba

text: leaf

text: Populus alba leaf 2 year old plant


text: The Institute for Genomic Research

text: live

text: Generic

text: Female, 2 years; oligo-dT + random primed cDNA synthesis; lambdaZAP-II vector, 1.0kb average insert size.

characteristic: description

text: Homo sapiens

text: Hippocampus, Stratagene (cat. #936205)

text: Phage

text: lambdaZAP-II


text: USDA-ARS

text: live

text: Generic

characteristic: NCBI submission model

text: Subtractive hybridization was performed with the PCR-Select cDNA Subtraction Kit (Clontech), according to the manufacture's protocol. Tester tissue consisted of the anterior kidney of fish 48 hours post vaccination with Edwardsiella ictaluri RE-33 vaccine for 30 minutes. Driver tissue was the anterior kidney of non-vaccinated fish.

text: 8 months

text: anterior kidney

text: Ictalurus punctatus

text: mixed

text: Channel catfish anterior kidney subtractive library 48h post vaccination with Edwardsiella ictaluri RE-33 vaccine

text: pGEM T easy (promega)


text: Kansas State University

text: live

text: Generic

text: cDNA library of primary reproductive dispersers. Sequences from the whole body of adult eastern subterranean termites.

text: Adult Alates

text: E. coli

characteristic: lab-host

text: Reticulitermes flavipes

text: EcoRV

text: unsexed

text: laboratory strain

text: whole body

characteristic: tissue-type

text: library of adult alate termite

text: pBluescript II SK+


text: Kansas State University

text: live

text: Generic

characteristic: NCBI submission package

text: cDNA library of early larva. Sequences from the whole body of subterranean termites.

text: Early Larva

text: E. coli

text: Reticulitermes flavipes

text: EcoRV

text: unsexed

text: laboratory strain

text: whole body

text: library of early larva termite

text: pBluescript II SK+

characteristic: vector


text: Kansas State University

text: live

text: Generic

text: cDNA library of late larva. Sequences from the whole body of subterranean termites.

text: Late Larva

text: E. coli

text: Reticulitermes flavipes

characteristic: organism

text: EcoRV

text: unsexed

text: laboratory strain

text: whole body

text: library of late larva termite

text: pBluescript II SK+


text: Kansas State University

text: live

text: Generic

text: cDNA library of soldier. Squences from the whole body of adult eastern subterranean termites.

text: Adult Soldier

text: E. coli

text: Reticulitermes flavipes

text: EcoRV

text: unsexed

characteristic: sex

text: laboratory strain

text: whole body

text: library of adult soldier termite

text: pBluescript II SK+


characteristic: External Id

text: Kansas State University

text: live

text: Generic

text: cDNA library of worker. Sequences from the whole body of adult eastern subterranean termites.

text: Adult Worker

text: E. coli

text: Reticulitermes flavipes

text: EcoRV

text: unsexed

text: laboratory strain

text: whole body

text: library of adult Worker termite

text: pBluescript II SK+


text: Centre for Environmental Stress and Adaptation Research, Bio21 Institute, Genetics Department, University of Melbourne

characteristic: INSDC center name

text: live

text: Generic

text: Total RNA was extracted using Trizol (Invitrogen). First and second strand cDNA were prepared using the Creator SMART cDNA Library Construction Kit LD PCR protocol (Clontech). Double stranded cDNA was purified using the DNA Clean And Concentrator (Zymogen) and digested with Sfi-I restriction enzyme. Digested cDNA was purified and ligated to pDNR-Lib vector (Clontech) and heat transformed into DH5alpha cells. Plasmids were sequenced using M13 primers (M113_forward: 5'GTAAAACGACGGCCAGT3' and M13_reverse: 5'AACAGCTATGACCATG3').

text: adult

text: Drosophila birchii

text: Sfi-I A

text: Sfi-I B

text: female and male

text: whole body

text: Drosophila birchii adult whole body cDNA library

text: plasmid

text: pDNR-LIB


text: Stony Brook University

text: live

text: Generic

text: Mercenaria mercenaria exposed to QPX infection

text: adults

text: Mercenaria mercenaria

text: gill, mantle and hemocytes

text: subtracted libraries from clams challenged with QPX

characteristic: title


text: Uppsala University

characteristic: INSDC first public

text: live

text: Generic

text: E. coli Top 10

text: Sparus aurata

text: gill

text: confinement gill early(fw+rv) (gsug02)

text: plasmid

text: PCR 2.1


characteristic: INSDC last update

text: live

text: Generic

text: E. coli Top 10

text: Sparus aurata

text: head kidney

text: Aquafirst Sea Bream SSH selection pathogen head kidney (gsgy08)

text: plasmid

text: PCR 2.1


text: live

text: Generic

text: E. coli Top 10

text: Sparus aurata

text: head kidney

text: Aquafirst Sea Bream SSH selection confinement head kidney late (gsgy03)

text: plasmid

text: PCR 2.1


text: National University of Ireland Galway (NUIG)

text: live

text: Generic

text: E. coli Top 10

text: Sparus aurata

text: head kidney

text: Aquafirst Sea Bream SSH selection confinement head kidney late (gsgy04)

text: plasmid

text: PCR 2.1


text: live

text: Generic

text: E. coli Top 10

text: Sparus aurata

text: head kidney

text: Aquafirst Sea Bream SSH selection confinement head kidney early (gsgy02)

text: plasmid

text: PCR 2.1


text: Uppsala University

text: live

text: Generic

text: E. coli Top 10

text: Sparus aurata

text: brain

text: confinement brain early (fw+rv) (gsub02)

text: plasmid

text: PCR 2.1


text: Uppsala University

text: live

text: Generic

text: E. coli Top 10

text: Sparus aurata

text: brain

text: confinement brain early (fw+rv) (gsub01)

text: plasmid

text: PCR 2.1


text: live

text: Generic

text: E. coli Top 10

text: Sparus aurata

text: liver

text: Aquafirst Sea Bream SSH selection confinement liver late (gsgl04)

text: plasmid

text: PCR 2.1