text: National Eye Institute

text: live

text: Generic

text: RNA was extracted from pooled mouse retina. A directionally cloned cDNA library in the pGADT7-rec vector was constructed at Clontech (Laurel MD). EST analysis was performed at the NIH Intramural Sequencing Center (NISC). Analyzed data available through http://neibank.nei.nih.gov.

text: adult

characteristic: dev-stage

text: Eye

characteristic: organ

text: Mus musculus

text: Retina

text: Mouse retina. Y2H (nbk)

text: Plasmid


status: PUBLIC

characteristic: External Id

text: Kasetsart University

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: The cDNA library was generated as part of Macrobrachium rosenbergii EST project. Total RNA was extracted from hemocytes of Macrobrachium rosenbergii, which were fed with 5g Beta-glucan/kg diet for 14 days. The mRNA was further purified, reverse transcribed and cloned into Uni-ZAP XR vector.

characteristic: description

text: Macrobrachium rosenbergii

characteristic: organism

text: EcoR I

tag: attribute

text: Xho I

text: Hemocytes

characteristic: tissue-type

text: Beta-glucan stimulated hemocyte library

characteristic: title

text: Phage

text: Uni-ZAP XR

characteristic: vector


text: Dana Farber Cancer Institute

text: live

text: Generic

text: The ORFs were PCR amplified from the MGC (Mammalian Gene Collection) and cloned by recombinational Gateway cloning into pDONR223 Donor vector. Reference : MGC (Mammalian Gene Collection) Program Team, Generation and Initial Analysis of more than 15,000 Full-Length Human and Mouse cDNA Sequences. PNAS, 2002, 99(26), 16899-16903

text: Homo sapiens

text: mixed

text: Full Length cDNA from the Mammalian Gene Collection

text: Plasmid

text: mixed


text: Universite Libre de Bruxelles

text: live

text: Generic

text: clone INRA 717-1B4

characteristic: cultivar

text: a transcriptional comparison between two different developmental stages along aspen stems (top and bottom tissue samples of 6-month-old plants) was performed by cDNA-amplified length polymorphism (cDNA-AFLP). The top sample (Pta top) corresponds to the transition from primary to secondary growth, whereas the bottom sample (Pta bottom) corresponds to established secondary growth.

text: Secondary growth

text: E. coli TOP10

characteristic: lab-host

text: Stem

text: Populus tremula x Populus alba

text: Female

characteristic: sex

text: Vascular tissues

text: Pta bottom

text: Plasmid


text: Universite Libre de Bruxelles

text: live

text: Generic

text: clone INRA 717-1B4

text: Transition primary/secondary growth

characteristic: dev-stage

text: E. coli TOP10

text: Stem

text: Populus tremula x Populus alba

text: Female

text: Vascular tissues

text: Pta top

text: Plasmid


text: Sri Krishnadevaraya University

text: live

text: Generic

text: Seeds were surface sterilized with 0.1% (w/v) sodium hypo chlorite solution for 5 min, thoroughly rinsed with distilled water and germinated in plastic pots contained soil and sand (2:1) mixture and allowed to grow under natural photoperiod (10-12 hrs, 28 +/- 4 o C) in the departmental botanic garden. Seedlings were maintained at 100% soil moisture levels (SMLs) by watering daily. Nineteen day-old plants were subjected to moisture stress by with holding water. Leaf samples collected from unstressed (100% SMLs) and stressed (25% SMLs) plants, flash frozen and stored at -80oC. The frozen samples were used for RNA extraction (LiCl2 method). Poly(A) mRNA was isolated using streptavidin paramagnetic particles (Roche, Germany). First-strand cDNA synthesis was initiated from the enriched mRNA of driver (unstressed) using biotin labeled poly dT and Superscript RNaseH- reverse transcriptase (Gibco-BRL, USA). Subtractive hybridization was performed between biotin labeled driver sscDNA (unstressed) and tester (stressed) mRNA at 65oC for 1 hour. The tester-driver hybrids and excess of the driver were separated from the unhybridized tester mRNA using streptavidin paramagnetic particles. Tester mRNAs that did not hybridize with the driver were used for cDNA synthesis (Stratagene). A primary cDNA library was produced using a lambda ZAP XR cDNA synthesis kit (Stratagene). Randomly selected clones amplified using M13F and M13R-primers, PCR product was purified using PCR purification system (Stratagene) and sequenced from the 3' end on an MegaBACE 1000 DNA analysis system (Amersham Pharmacia, Uppsala, Sweden). Sequences are processed using the VecScreen (http://www.ncbi.nlm.nih.gov/VecScreen.html) to remove vector and cloning oligo sequences and various contaminants, and to trim to a high quality region. Sequences that retained at least 100 bases were submitted to GenBank.

text: 21 day-old seedlings

text: E.coli

text: Carthamus tinctorius

text: EcoRI

text: XhoI

text: Leaf

text: Safflower (A-1) drought stressed subtracted cDNA library

text: Lambda Uni-ZAP XR


text: NRC Institute for Marine Biosciences Industrial Partnership Facility

text: live

text: Generic

text: Gadus morhua

text: mixed tissue

text: Gadus morhua mixed tissue library nmplate


text: live

text: Generic

text: Gadus morhua

text: liver

text: Gadus morhua liver library (nodavirus infected) gmnsil


text: live

text: Generic

text: Gadus morhua

text: liver

text: Gadus morhua liver library gmnsul


text: Centre for Genetic Engineering and Biotechnology (CIGB)

text: live

text: Generic

text: Third instar

text: Spodoptera frugiperda

text: Midgut

text: Spodoptera frugiperda SSH cDNA Library


text: Hanyang University

text: live

text: Generic

text: Antarctic diatom Chaetoceros neogracile, Uni-ZAP XR Vector System (Stratagene)

text: E. coli SOLR

text: Chaetoceros neogracilis

text: EcoRI

text: XhoI

text: KOPRI AnM0002

characteristic: strain

text: Chaetoceros neogracile EST Library

text: phagemid

text: pBluescript SK-


characteristic: External Id

text: Institute of Plant Physiology & Ecology, Chinese Academy of Sciences

text: live

text: Generic

text: the library is a mixture of 3-9 day after pollination Brassica Napus seed

text: seed

characteristic: organ

text: Brassica napus

text: SfiIA

text: SfiIB

text: hermaphrodite

text: Huyou

text: 3-9 DAP Brassica Napus seed cDNA library

text: Plasmid

text: pBSF


characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: the library is a mixture of 11-19 day after pollination Brassica Napus seed

text: seed

text: Brassica napus

text: SfiIA

text: SfiIB

text: hermaphrodite

text: Huyou

text: 11-19 DAP Brassica Napus seed cDNA library

text: Plasmid

text: pBSF


text: Boston University

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Nematostella vectensis

text: Finnerty Lab Nematostella vectensis cDNA library


text: Rajiv Gandhi Centre for Biotechnology

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Rio-de-Janeiro

text: RNA extracted from leaves using Trizol reagent. cDNA synthesized using 15-mer oligo dT and MMLV-RT (Promega). cDNA amplified with Zingiber RGC specific primers.

text: Zingiber officinale

text: leaves of Zingiber officinale cv. Rio-de-Janeiro


text: Rajiv Gandhi Centre for Biotechnology

text: live

text: Generic

text: wild

characteristic: description

text: Zingiber zerumbet

characteristic: organism

text: leaves of Zingiber zerumbet


text: Shanghai Institute of Plant Physiology and Ecology

text: live

text: Generic

text: larval stages

text: Bombyx mori

text: silkgland

characteristic: tissue-type

text: silkworm silkgland 3' EST

characteristic: title


text: IBP-CNRS, University of Paris XI

text: live

text: Generic

text: INRA hybrid, clone 717-1-B4

characteristic: cultivar

text: 3-month-old in vitro plants

text: shoot apex

text: Populus tremula x Populus alba

text: female

text: shoot apex

text: PCR amplification from Populus tremula x Populus alba cDNA


text: Padua University

text: live

text: Generic

text: Salix matsudana

text: root

text: Salix matsudana root


text: Padua University

text: live

text: Generic

text: Salix fragilis

text: roots

text: Salix fragilis roots


text: Padua University

text: live

text: Generic

text: Salix matsudana

text: leaves

text: Salix matsudana leaves


text: Padua University

text: live

text: Generic

text: Salix alba

text: leaves

text: Salix alba leaves


text: Padua University

text: live

text: Generic

text: Salix elaeagnos

text: roots

text: Salix elaeagnos roots


text: Padua University

text: live

text: Generic

text: Salix alba

text: roots

text: Salix alba roots


text: Padua University

text: live

text: Generic

text: Salix elaeagnos

text: leaves

text: Salix elaeagnos leaves


text: Purdue University, Department of Horticulture and Landscape Architecture

text: live

text: Generic

text: P-starved seedlings

characteristic: cell type

text: Suppression subtractive cDNA library

text: 14-day-Phosphrous starved

text: Seedlings

text: Lolium multiflorum

text: bi-sexual

text: Gulf-grass

text: Shoots and roots

text: Lolium multiflorum suppression subtractive cDNA library

characteristic: vector


text: live

text: Generic

text: Ammopiptanthus mongolicus

text: leaf

text: Ammopiptanthus mongolicus leaf cDNA library


text: Sime Darby Technology Centre Sdn. Bhd.

text: live

text: Generic

text: Elaeis guineensis

text: XhoI

text: young flower 4-6 cm

text: Elaeis guineensis young flower 4-6 cm

text: phage

text: Uni-Zap(Stratagene)


text: Sime Darby Technology Centre Sdn. Bhd.

text: live

text: Generic

text: Elaeis guineensis

text: XhoI

text: mature flower 26 cm

text: Elaeis guineensis mature flower 26 cm

text: phage

text: Uni-Zap(Stratagene)


text: Sime Darby Technology Centre Sdn. Bhd.

text: live

text: Generic

text: Elaeis guineensis

text: XhoI

text: shoot apex

text: Elaeis guineensis shoot apex

text: phage

text: Uni-Zap(Stratagene)


text: Malaysian Palm Oil Board (MPOB)

text: live

text: Generic

text: Elaeis guineensis

text: XhoI

text: Suspension cell cultures

text: Elaeis guineensis Suspension cell cultures

text: phage

text: Uni-Zap(Stratagene)


text: Sime Plantations Sdn Bhd

text: live

text: Generic

text: Elaeis guineensis

text: XhoI

text: root

text: Elaeis guineensis root

text: phage

text: Uni-Zap(Stratagene)


text: Universiti Putra Malaysia

text: live

text: Generic

text: Elaeis guineensis

text: XhoI

text: zygotic embryo

text: Elaeis guineensis zygotic embryo

text: phage

text: Uni-Zap(Stratagene)


text: Kasetsart University

text: live

text: Generic

text: The cDNA library was generated as part of Macrobrachium rosenbergii EST project. Total RNA was extracted from hemocytes of normal Macrobrachium rosenbergii. The mRNA was further purified, reverse transcribed and cloned into Uni-ZAP XR vector.

text: Macrobrachium rosenbergii

text: EcoR I

text: Xho I

text: Hemocytes

text: Macrobrachium rosenbergii Normal hemocyte library

text: Phage

text: Uni-ZAP XR


text: live

text: Generic

text: Horizontal resistance potato material

text: Detached leaves were challenged by P.infestans with 1x10 5 sporangia/mL or treated with 2 mM DL-beta-amino-butyric acid. Detached leaf samples were individually collected at 2, 4, 6, 8, 12, 16, 20, 24, 30, 36, 48, 60 hours and frozen in liquid nitrogen immediately

text: 8 weeks old

characteristic: dev-stage

characteristic: lab-host

text: Solanum tuberosum

text: leaf

text: potato ESTs differentially expressed after Phytophthora infestans-challenge or DL-beta-amino-butyric acid treatments


characteristic: External Id

text: University of Hawaii at Manoa

text: live

text: Generic

text: The cDNA library was established by total RNA isolated from SPF-free juvenile pacific white, and smart cDNA construction kit.

text: 1 month of age

text: Penaeus vannamei

text: Abdominal muscle

text: Litopenaeus vannamei 1 month SMART cDNA library


text: Graduate School of Science,, Hiroshima University

text: live

text: Generic

text: hybrid lethality related genes; suspension cultured cell

text: Nicotiana suaveolens x Nicotiana tabacum suppression subtractive hybridization


text: University of Trieste

text: live

text: Generic

text: Boubon Red and Catuai Red mixed

text: country: Italy

text: Coffea arabica

text: embryonic root

text: Coffea arabica embryonic root


text: University of Trieste

text: live

text: Generic

text: Bourbon Tekicic

text: country: Italy

text: Coffea arabica

text: leaf

text: Coffea arabica leaf


text: Instituto de Biologia Molecular y Celular de Plantas (IBMCP)

text: live

text: Generic

text: cDNA-AFLP fragment

text: Solanum tuberosum

text: leaf tissue and in vitro plantlets

text: potato leaf and in vitro plantlets


text: Alabama A&M University

text: live

text: Generic

text: 15 days post anthesis

text: E. coli

text: Gossypium hirsutum

text: ovule fiber

text: Gossypium hirsutum Fiber SSH cDNA Library

text: plasmid

text: pCR II-TOPO


text: NFSPRC Bldg.

text: live

text: Generic

text: Subtractive hybridization library derived from mature soil grown Lolium temulentum plants at the dough stage of seed development. Plant stems, leaves and crowns were subjected to a simulated grass seed harvest and subsequent field dehydration. Products were TA cloned into vector and sequenced with T7 Primer.

text: adult, flowering, plants grown under long days

text: E. coli, Top010

text: Lolium temulentum

text: stem, leaf and crown

text: Lolium temulentum post harvest subtraction library

text: Plasmid

text: pGEM T easy


text: Lund University

text: live

text: Generic

text: 4 weeks old plant

text: Nicotiana sylvestris

text: leaf

text: Nicotiana sylvestris 4 weeks old plant


text: Northeast Forestry University

text: live

text: Generic

text: SSH cDNA library in which the cDNA of Bursaphelenchus xylophilus was done as Tester and the cDNA of Bursaphelenchus mucronatus was done as Driver.

text: Bursaphelenchus xylophilus

text: SSH cDNA Library of Bursaphelenchus xylophilus


text: NI Vavilov Institute of General Genetics, Russian Academy of Sciences

text: live

text: Generic

text: 4 weeks old plant

text: lab line E9

characteristic: isolate

text: leaf

characteristic: organ

text: Arabidopsis thaliana

text: hermaphrodite

characteristic: sex

text: Arabidopsis thaliana E9-line mRNA

text: Activation tagging T-DNA Plasmid

text: pEnLox (DQ645630)


text: Universidad Nacional Autonoma de Mexico

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Larva

text: Taenia solium

text: Whole cysticercus

text: Taenia solium UNAM-cd2_larva

text: pBlueScript SK


text: Universidad Nacional Autonoma de Mexico

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Adult

text: Taenia solium

text: Whole tape-worm

text: Taenia solium UNAM-cd1_adult

text: pBlueScript SK


text: Faculty of Agriculture and Natural Science, University of Pretoria

text: live

text: Generic

text: cDNA-AFLP of wheat mRNA in response to infestation with the Russian wheat aphid (Diuraphis noxia)

characteristic: description

text: 2-3 leaf stage

text: Eschericia coli (DH5a)

text: leaf

text: Triticum aestivum

characteristic: organism

text: cDNA-AFLP of wheat 94M370 in response to Diuraphis noxia

text: Plasmid


text: University of Agricultural Sciences

text: live

text: Generic

text: modern

characteristic: cultivar

text: sunflower 20-day-old seedlings were subjected to different water stress (40%F.C, 60%F.C) by withholding water till the crop plants withstands to the respective stress level. RNA isolated from leaf tissues. Isolated cDNA was cloned using TA cloning kit.

text: 25 day-old seedlings

text: E.coli

text: Helianthus annuus

text: EcoRI

text: Leaf

characteristic: tissue-type

text: Sunflower drought stress cDNA library

characteristic: title

text: TA cloning vector


text: Northwest A & F University

text: live

text: Generic

text: young leaves

characteristic: cell type

text: Total RNA was extracted by Trizol, then reverse transcription was conducted by AMV reverse transcriptase; then double strand cDNA was synthesized and subjected to SSH analysis by using PCR Select cDNA Subtraction Kit (Clontech company) after twice hybridization. The PCR products were purified and cloned into pGEM-T Easy vector and transformed to E coli strain DH5a, sequencing were conducted by Sp6 or T7 primer at LiCOR 4200 DNA Sequencer.

text: stage at 4-5 leaves, after 2 days inoculation with Stripe rust pathogen 32

characteristic: dev-stage

text: Triticum aestivum

text: leaves

text: SSH-cDNA Library of stripe rust infection in leaves of stripe rust resistant Shaanmai 139 compared with no infection


characteristic: External Id

text: Northeast Forestry University

text: live

text: Generic

text: up-regulated genes in salt stress. country: China:Heilongjiang.

text: Chloris virgata

text: shoot

text: Chloris virgata Shoot cDNA


text: First Hospital of Shanxi Medical University

text: live

text: Generic

text: We obtained this sequence from high-throughput cDNA microarrays of invasive cervical carcinoma of FIGO stage Ib with lymph node metastases and without lymph node metastases. It has been verified in tissue of sqamous carcinoma of the cervix.

text: adult

text: E.coli DH5a

characteristic: lab-host

text: cervix

text: Homo sapiens

text: female

text: sqamous carcinoma of the cervix

text: Homo sapiens sqamous carcinoma of the cervix Express Library

text: pMD 18-T

characteristic: vector


text: National Cancer Institute / NIH

text: live

text: Generic

text: RNA obtained from 8 wk old animal. Tissues were snap-frozen and kept at -80C before RNA extraction and purification (Tri-reagent method). cDNA was primed using oligo-dT primer: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3' and cloned into the EcoRV/NotI sites of pExpress-1. Size-selection >1.25kb resulted in an average insert size of 1.9 kb. This is a primary library (normalized library is NIH_MGC_251) and was constructed by Open Biosystems. Note: this is a NIH_MGC library

text: DH10B TonA

text: thymus

characteristic: organ

text: Rattus norvegicus

text: EcoRV

text: NotI

text: plasmid


text: National Cancer Institute / NIH

characteristic: INSDC center name

text: live

text: Generic

text: RNA obtained from 8 wk old animal. Tissues were snap-frozen and kept at -80C before RNA extraction and purification (Tri-reagent method). cDNA was primed using oligo-dT primer: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3' and cloned into the EcoRV/NotI sites of pExpress-1. Size-selection >1.25kb resulted in an average insert size of 1.6 kb. This primary library is normalized (non-normalized primary library is NIH_MGC_250) and was constructed by Open Biosystems. Note: this is a NIH_MGC library

text: DH10B TonA

text: thymus

text: Rattus norvegicus

text: EcoRV

text: NotI

text: plasmid


text: University of Minnesota

characteristic: INSDC first public

text: live

text: Generic

text: Meleagris gallopavo

text: skeletal muscle

text: Meleagris gallopavo 16wk_adult

text: plasmid


text: University of Minnesota

characteristic: INSDC last update

text: live

text: Generic

text: Meleagris gallopavo

text: skeletal muscle

text: Meleagris gallopavo 1DPH

text: plasmid


text: University of Hawaii at Manoa

text: live

characteristic: INSDC status

text: Generic

text: Carica papaya

text: Carica papaya cDNA

text: Lambda Zap


text: INRA UMR1064 Plant-microbe interactions and plant health

text: live

text: Generic

characteristic: NCBI submission model

text: The cDNA library was made from mRNAs extracted from J3 endophte stages

text: Meloidogyne incognita

text: Meloidogyne incognita J3-enriched cDNA library

text: Plasmid

text: pGEMT


text: Technion Israel Institute of Technology

text: live

text: Generic

characteristic: NCBI submission package

text: adult

text: Botrylloides leachii

text: hermaphrodite

characteristic: sex

text: Botrylloides regeneration subtraction Library

text: Plasmid


text: National Institute for Viral Disease Control and Prevention

text: live

text: Generic

text: The cDNA library came from mixture of Trichophyton rubrum mycelia collected from keratin, elastin and collagen salt medium, respectively. The cDNA library was constructed following the protocols of the SUPERSCRIPT Plasmid System with GATEWAY Technology for cDNA Synthesis and Cloning (Invitrogen).

characteristic: description

text: Trichophyton rubrum

characteristic: organism

text: SalI

text: NotI

characteristic: strain

text: Trichophyton rubrum cDNA library 10

text: plasmid


text: live

text: Generic

text: The cDNA library came from Trichophyton rubrum mycelia collected from collagen salt medium at 28oC. The cDNA library was constructed following the protocols of the SUPERSCRIPT Plasmid System with GATEWAY Technology for cDNA Synthesis and Cloning (Invitrogen).

text: Trichophyton rubrum

text: SalI

text: NotI

text: Trichophyton rubrum cDNA library 11

characteristic: title

text: plasmid


text: live

text: Generic

text: The cDNA library came from Trichophyton rubrum mycelia collected from elastin salt medium at 28oC. The cDNA library was constructed following the protocols of the SUPERSCRIPT Plasmid System with GATEWAY Technology for cDNA Synthesis and Cloning (Invitrogen).

text: Trichophyton rubrum

text: SalI

text: NotI

text: Trichophyton rubrum cDNA library 12

text: plasmid


text: live

text: Generic

text: The cDNA library came from Trichophyton rubrum mycelia collected from keratin salt medium at 28oC. The cDNA library was constructed following the protocols of the SUPERSCRIPT Plasmid System with GATEWAY Technology for cDNA Synthesis and Cloning (Invitrogen).

text: Trichophyton rubrum

text: SalI

text: NotI

text: Trichophyton rubrum cDNA library 13

text: plasmid


text: Michigan State University

text: live

text: Generic

text: hemocytes

characteristic: cell type

text: Dreissena polymorpha

text: Dreissena polymorpha stimulated with lipopolysaccharide, peptidoglycan and zymosan


text: live

text: Generic

text: Obtaining this sequence is casual in procedure of rapid amplification of another EST. We utilized a 5'end specific primer and an universal primer obtain this whole sequence. After blasting, we find it is a new EST. It has been verified in tissue of sqamous carcinoma of the cervix.

text: adult

characteristic: dev-stage

text: E.coli DH5a

text: cervix

text: Homo sapiens

text: female

text: sqamous carcinoma of the cervix

characteristic: tissue-type

text: Homo sapiens sqamous carcinoma of the cervix Express Library 2

text: pMD 18-T


characteristic: External Id

text: First Hospital of Shanxi Medical University

text: live

text: Generic

text: We obtained this EST from high-throughput cDNA microarrays of invasive cervical carcinoma of FIGO stage Ib with lymph node metastases and without lymph node metastases. Then uesed RT-PCR certified it in the tissues of sqamous carcinoma of the cervix.

text: adult

text: E.coli DH5a

characteristic: lab-host

text: cervix

text: Homo sapiens

text: female

text: sqamous carcinoma of the cervix

text: Homo sapiens sqamous carcinoma of the cervix Express Library 3

text: pMD 18-T


text: Cinvestav Irapuato

text: live

text: Generic

text: Serrano Tampiqueno 74

characteristic: cultivar

text: Arabidopsis thaliana cv. Columbia plants and Capsicum annuum cv. Serrano Tampiqueno 74 plants were grown in a growth chamber at 27ÂoC Â± 1ÂoC with a photoperiod 18 h light/6 h. The cDNA library was made from RNA extracted from pooled flowers including from bud to postanthesis stages of each plant. Suppression subtraction hybridization was performed following the instructions of the PCR-Select cDNA subtraction kit (Clontech 637401 Mountain View, CA). We utilized a SSH protocol to subtract driver cDNAs (A. thaliana) from tester cDNAs (C. annuum) in order to obtain those cDNAs unique to the tester cDNA population. 2.5 micrograms each of tester and driver mRNA were used to proceed with the subtraction. The subtracted product was used to construct a cDNA library. PCR products obtained by the second hybridization were inserted into pJET1 (Fermentas Vilnius, Lt.).

text: flower

characteristic: organ

text: Capsicum annuum

text: SSH library of flowers of Capsicum - Arabidopsis

characteristic: vector


text: NRC on Plant Biotechnology, IARI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Pusa362 & SBD377

text: Templates for the DDRT-PCR were reverse-transcribed from mRNA extracted (1) from drought resistant chickpea cultivar (PUSA 362) & drought susceptible (SBD377) control plant and (2) from drought resistant chickpea cultivar (PUSA 362) & drought susceptible (SBD377) dehydration stressed plant. Transcripts profiles were compared between (1) and (2). cDNAs with differential expression were cloned in pGEM-T EASY vector & sequenced using T7 sequencing primer.

text: Seedling stage

text: Cicer arietinum

text: Differentially expressed genes in chickpea under dehydration stress

text: pGEM-T EASY


text: Lawrence Berkeley National Lab

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: iPCR amplified cDNA clones using gene specific primers from a pool of GH (head), LD (embryo), LP (larva and pupa), and SD (S2 cell line) libraries. Since clones in this library have been enhanced for specific targets by PCR, they cannot be used to quantify mRNA expression levels.

text: mixed

text: mixed

text: Drosophila melanogaster

text: EcoRI

text: male and female

text: IP Drosophila melanogaster iPCR Amplified Library


text: UC Davis, Department of Pomology

text: live

text: Generic

text: On March 11, 2006, carrot discs were inoculated with Pratylenchus vulnus from an in vitro culture on walnut roots. On April 28, 2006, these nematodes were harvested by soaking the carrot discs in 1/4 strength M9 media for 3 hours. Nematodes were packed by centrifugation and total RNA was immediately isolated (TriZol reagent; Invitrogen CA). Total RNA was purified (Qiagen RNA/DNA Mini Kit) and used to construct a cDNA library (CLONTECH Creator SMART cDNA Library Kit). Ligated plasmids were electroporated into E. coli DH10-Beta (Invitrogen, CA) to obtain individual colonies that were picked and sequenced using plasmid primers to obtain 5' ESTs (UC Davis CA&ES Core Genomics Facility).

characteristic: description

text: Mixed

text: E. coli DH10-Beta

text: Pratylenchus vulnus

characteristic: organism

text: SfiIA

text: SfiIB

text: Plasmid

text: pDNR-LIB


text: UC Davis, Department of Pomology

text: live

text: Generic

text: Paradox clone 'PX1'

text: Five micropropagated plants of the walnut paradox clone PX1 were grown in the greenhouse. On April 5, 2006 each plant was inoculated with ~6400 individual Pratylenchus vulnus nematodes. On May 19, 2006 the leaves of these plants were harvested and frozen in liquid nitrogen and stored at -80C. RNA was isolated from the leaves obtained from the individual plants using the hot borate method. Total RNA was purified (Qiagen RNA/DNA Mini Kit) from each plant, pooled and used to construct a cDNA library (CLONTECH Creator SMART cDNA Library Kit). Ligated plasmids were electroporated into E. coli DH10-Beta (Invitrogen) to obtain individual colonies that were sequenced using plasmid primers to obtain 5' ESTs (UC Davis CA&ES Core Genomics Facility).

text: Small greenhouse plants

text: E. coli DH10-Beta

text: Root

text: Juglans hindsii x Juglans regia

text: SfiIA

text: SfiIB

text: WLN-02: walnut leaf from plants infected with Pratylenchus vulnus

characteristic: title

text: Plasmid

text: pDNR-LIB


text: UC Davis, Department of Pomology

text: live

text: Generic

text: Paradox clone 'PX1'

text: Five micropropagated plants of the walnut Paradox clone PX1 were grown in the greenhouse. On May 19, 2006 the leaves of these plants were harvested, frozen in liquid nitrogen and stored at -80C. RNA was isolated from the leaves obtained from the individual plants using the hot borate method. Total RNA was purified (Qiagen RNA/DNA Mini Kit)from each plant, pooled and used to construct a cDNA library (CLONTECH Creator SMART cDNA Library Kit). Ligated plasmids were electroporated into E. coli DH10-Beta (Invitrogen) to yield individual colonies that were picked and sequenced using plasmid primers to obtain 5' ESTs (UC Davis CA&ES Core Genomics Facility).

text: Small greenhouse plants

text: E. coli DH10-Beta

text: Leaf

text: Juglans hindsii x Juglans regia

text: SfiIA

text: SfiIB

text: WLO-02: Walnut leaf

text: Plasmid

text: pDNR-LIB


text: UC Davis, Department of Pomology

text: live

text: Generic

text: Paradox clone 'PX1'

text: Roots of germinated somatic embryos on DKW media in culture plates were inoculated with Pratylenchus vulnus. Four months later, total RNA was isolated using the hot borate method. RNA was purified (Qiagen RNA/DNA Mini Kit) and was used to construct a cDNA library (CLONTECH SMART cDNA Library kit). The primary library was en masse evicted and plasmid DNA containing the cDNA library was isolated form the resultant bacterial population. Plasmid DNA from individual transformed colonies was sequenced using plasmid primer sequences (Washington University Genome Sequence Center, St. Louis, MO).

text: In vitro grown roots from germinated somatic embryos

text: Root

text: Juglans hindsii x Juglans regia

text: SfiIA

text: SfiIB

text: WRN-01: Walnut root infected with Pratylenchus vulnus

text: Plasmid

text: Lambda TriplEX2


text: UC Davis, Department of Pomology

text: live

text: Generic

text: Paradox clone 'PX1'

text: Five micropropagated plants of the walnut Paradox clone PX1 were grown in the greenhouse. On April 5, 2006 each plant was inoculated with approximately 6400 individual Pratylenchus vulnus nematodes. On May 19, 2006 the roots of these plants were harvested, frozen in liquid nitrogen and stored at -80C. RNA was isolated from individual plants using the hot borate method. Total RNA was purified (Qiagen RNA/DNA Mini Kit) from each plant, pooled and used to construct a cDNA library (CLONTECH Creator SMART cDNA Library Kit). Ligated plasmids were electroporated into E. coli DH10-Beta (Invitrogen). Individual colonies were picked and sequenced using plasmid primers to obtain 5' ESTs (UC Davis CA&ES Core Genomics Facility).

text: Small greenhouse plants

text: E. coli DH10-Beta

text: Root

text: Juglans hindsii x Juglans regia

text: SfiIA

text: SfiIB

text: WRN-02: Walnut root infected with Pratylenchus vulnus

text: Plasmid

text: pDNR-LIB


text: UC Davis, Department of Pomology

text: live

text: Generic

text: Paradox clone 'PX1'

text: Total RNA was isolated using the hot borate method from roots of germinated somatic embryos on DKW media in culture plates. Total RNA was purified (Qiagen RNA/DNA Mini Kit) and used to construct a cDNA library (CLONTECH SMART cDNA Library kit). The primary library was en masse evicted and plasmid DNA containing the cDNA library was isolated form the resultant bacterial population. Plasmid DNA from individual transformed colonies was sequenced using plasmid primer sequences (Washington University Genome Sequence Center, St. Louis, MO).

text: Root

text: Juglans hindsii x Juglans regia

text: SfiIA

text: SfiIB

text: WRO-01: Walnut root

text: Plasmid

text: Lambda TriplEX2


text: UC Davis, Department of Pomology

text: live

text: Generic

text: Paradox clone 'PX1'

text: Five micropropagated plants of the walnut Paradox clone PX1 were grown in the greenhouse. On May 19, 2006 the roots of these plants were harvested, frozen in liquid nitrogen and stored at -80C. RNA was isolated from individual plants using the hot borate method. Total RNA was purified (Qiagen RNA/DNA Mini Kit) from each plant, pooled and used to construct a cDNA library (CLONTECH Creator SMART cDNA Library kit). Ligated plasmids were electroporated into E. coli DH10-Beta (Invitrogen). Individual colonies were picked and sequenced using plasmid primers to obtain 5' ESTs (UC Davis CA&ES Core Genomics Facility).

text: Small greenhouse plants

text: E. coli DH10-Beta

text: Root

text: Juglans hindsii x Juglans regia

text: SfiIA

text: SfiIB

text: WRO-02: Walnut root

text: Plasmid

text: pDNR-LIB


text: live

text: Generic

text: Gossypium hirsutum

text: leaf

text: cotton leaves expressed sequences


text: Cornell University

text: live

text: Generic

text: The library was prepared from polyA+ RNA from strain G5 trophonts treated with i-antigen specific antibodies to induce immune stress and the physiological responses associated with premature exit from the host. Double-stranded cDNA was cloned unidirectionally into different DraIII sites of the pME18S-FL3 vector (5-prime DraIII site is CACTGTGTG, 3-prime DraIII site is CACCATGTG). XhoI excises the cDNA insert.

text: Trophont

text: DH10B-T1 phage-resistant E. coli

text: Ichthyophthirius multifiliis

text: XhoI

text: XhoI

characteristic: strain

text: G5 antibody-treated trophants

text: Plasmid


text: Cornell University

text: live

text: Generic

text: The library was prepared from polyA+ RNA from strain G5 theronts. Double-stranded cDNA was cloned unidirectionally into different DraIII sites of the pME18S-FL3 vector (5-prime DraIII site is CACTGTGTG, 3-prime DraIII site is CACCATGTG). XhoI excises the cDNA insert.

text: Theront

characteristic: dev-stage

text: DH10B-T1 phage-resistant E. coli

text: Ichthyophthirius multifiliis

text: XhoI

text: XhoI

text: G5 theronts: 1.25-3 kb fraction

text: Plasmid


text: Cornell University

text: live

text: Generic

text: The library was prepared from polyA+ RNA from strain G5 theronts. cDNA inserts were size-selected; this library represents the 0.8-1.25 kb fraction. Double-stranded cDNA was cloned unidirectionally into different DraIII sites of the pME18S-FL3 vector (5-prime DraIII site is CACTGTGTG, 3-prime DraIII site is CACCATGTG). XhoI excises the cDNA insert.

text: Theront

text: DH10B-T1 phage-resistant E. coli

text: Ichthyophthirius multifiliis

text: XhoI

text: XhoI

text: G5 theronts: 0.8-1.25 kb fraction

text: Plasmid


text: Cornell University

text: live

text: Generic

text: The library was prepared from polyA+ RNA from strain G5 theronts. cDNA inserts were size-selected; this library represents the 3-8 kb fraction. Double-stranded cDNA was cloned unidirectionally into different DraIII sites of the pME18S-FL3 vector (5-prime DraIII site is CACTGTGTG, 3-prime DraIII site is CACCATGTG). XhoI excises the cDNA insert.

text: Theront

text: DH10B-T1 phage-resistant E. coli

text: Ichthyophthirius multifiliis

text: XhoI

text: XhoI

text: G5 theronts: 3-8 kb fraction

text: Plasmid


text: Cornell University

text: live

text: Generic

text: The library was prepared from polyA+ RNA from strain G5 theronts. This library was amplified prior to selecting clones for sequencing. Double-stranded cDNA was cloned unidirectionally into different SfiI sites of the pTriplEx2 vector (5-prime SfiI site is GGCCATTATGGCC, 3-prime SfiI site is GGCCGCCTCGGCC). EcoRI and XhoI excise the cDNA insert.

text: Theront

text: Ichthyophthirius multifiliis

text: EcoRI

text: XhoI

text: G5 theronts: amplified library

text: Plasmid


text: Cornell University

text: live

text: Generic

text: The library was prepared from polyA+ RNA from strain G5 tomites. cDNA inserts were size-selected; this library represents the 1.25-3 kb fraction. Double-stranded cDNA was cloned unidirectionally into different DraIII sites of the pME18S-FL3 vector (5-prime DraIII site is CACTGTGTG, 3-prime DraIII site is CACCATGTG). XhoI excises the cDNA insert.

text: Tomite

text: DH10B-T1 phage-resistant E. coli

text: Ichthyophthirius multifiliis

text: XhoI

text: XhoI

text: G5 tomites: 1.25 - 3 kb fraction

text: Plasmid


text: Cornell University

text: live

text: Generic

text: The library was prepared from polyA+ RNA from strain G5 tomites. cDNA inserts were size-selected; this library represents the 3-8 kb fraction. Double-stranded cDNA was cloned unidirectionally into different DraIII sites of the pME18S-FL3 vector (5-prime DraIII site is CACTGTGTG, 3-prime DraIII site is CACCATGTG). XhoI excises the cDNA insert.

text: Tomite

text: DH10B-T1 phage-resistant E. coli

text: Ichthyophthirius multifiliis

text: XhoI

text: XhoI

text: G5 tomites: 3 to 8 kb fraction

text: Plasmid


text: Cornell University

text: live

text: Generic

text: The library was prepared from polyA+ RNA from strain G5 tomites. cDNA inserts were size-selected; this library represents a mixture of size fractions. Double-stranded cDNA was cloned unidirectionally into different DraIII sites of the pME18S-FL3 vector (5-prime DraIII site is CACTGTGTG, 3-prime DraIII site is CACCATGTG). XhoI excises the cDNA insert.

text: Tomite

text: DH10B-T1 phage-resistant E. coli

text: Ichthyophthirius multifiliis

text: XhoI

text: XhoI

text: G5 tomites: mixed sizes

text: Plasmid


text: Cornell University

text: live

text: Generic

text: The library was made from trophont polyA+ RNA of the G5 parasite strain. Double-stranded cDNA was linked to EcoRI adaptors, size fractionated, and material >500 bp cloned into lambda ZAP II. Plasmid DNA for sequencing was prepared by mass excision.

text: Trophont

text: SOLR strain of E. coli

text: Ichthyophthirius multifiliis

text: EcoRI

text: EcoRI

text: G5 trophont cDNA (INIT2)

text: Plasmid

text: pBlueScriptSK-


text: Cornell University

text: live

text: Generic

text: The library was prepared from polyA+ RNA from strain NY4 theronts. Double-stranded cDNA was cloned unidirectionally into different DraIII sites of the pME18S-FL3 vector (5-prime DraIII site is CACTGTGTG, 3-prime DraIII site is CACCATGTG). XhoI excises the cDNA insert.

text: Theront

text: DH10B-T1 phage-resistant E. coli

text: Ichthyophthirius multifiliis

text: XhoI

text: XhoI

text: NY4 theronts

text: Plasmid


text: Cornell University

text: live

text: Generic

text: The library was prepared from polyA+ RNA from strain NY4 theronts. This library was amplified prior to selecting clones for sequencing. Double-stranded cDNA was cloned unidirectionally into different SfiI sites of the pTriplEx2 vector (5-prime SfiI site is GGCCATTATGGCC, 3-prime SfiI site is GGCCGCCTCGGCC). EcoRI and XhoI excise the cDNA insert.

text: Theront

text: DH10B-T1 phage-resistant E. coli

text: Ichthyophthirius multifiliis

text: EcoRI

text: XhoI

text: NY4 theronts: amplified library

text: Plasmid


text: Cornell University

text: live

text: Generic

text: The library was prepared from polyA+ RNA from strain NY4 trophonts. Double-stranded cDNA was cloned unidirectionally into different SfiI sites of the pTriplEx2 vector (5-prime SfiI site is GGCCATTATGGCC, 3-prime SfiI site is GGCCGCCTCGGCC). EcoRI and XhoI excise the cDNA insert.

text: Trophont

text: DH10B-T1 phage-resistant E. coli

text: Ichthyophthirius multifiliis

text: EcoRI

text: XhoI

text: NY4 trophonts (NY4Tr1)

text: Plasmid


text: Cornell University

text: live

text: Generic

text: The library was prepared from polyA+ RNA from strain NY4 trophonts. Double-stranded cDNA was cloned unidirectionally into different DraIII sites of the pME18S-FL3 vector (5-prime DraIII site is CACTGTGTG, 3-prime DraIII site is CACCATGTG). XhoI excises the cDNA insert.

text: Trophont

text: DH10B-T1 phage-resistant E. coli

text: Ichthyophthirius multifiliis

text: XhoI

text: XhoI

text: NY4 trophants (NY4Tr2)

text: Plasmid


text: Cornell University

text: live

text: Generic

text: Trophont

text: DH10B-T1 phage-resistant E. coli

text: Ichthyophthirius multifiliis

text: XhoI

text: XhoI

text: NY4 Trophonts

text: Plasmid


text: USDA-ARS

text: live

text: Generic

text: 4th and 5th instar larvae

text: XL1 Blue

text: midgut

text: Ostrinia nubilalis

text: male and female

characteristic: sex

text: bivoltine Z-pheromone strain

text: ARS-CICGRU ONmgEST

text: plasmid

text: pDNRLIB


characteristic: External Id

text: Northwest A & F University

text: live

text: Generic

text: young leaves

characteristic: cell type

text: Total RNA was extracted by Trizol, then reverse transcription was conducted by AMV reverse transcriptase; then double strand cDNA was synthesized and subjected to SSH analysis by using PCR Select cDNA Subtraction Kit (Clontech company) after twice hybridization. The PCR products were purified and cloned into pGEM-T Easy vector and transformed to E coli strain DH5a, sequencing were conducted by Sp6 or T7 primer at LiCOR 4200 DNA Sequencer.

text: stage at 4-5 leaves, after driver was inoculated Stripe rust pathogen CY32 in 2 days

text: Triticum aestivum

text: leaves

characteristic: tissue-type

text: SSH-cDNA Library of normal leaves of stripe rust resistant material Shaanmai 139 compared with stripe rust infection


text: Institute of medical biology, Middle school attached to Yunnan normal university,Yunnan university second school

text: live

text: Generic

text: Mus musculus

text: male

text: NOD

text: kidney

text: Mus musculus strain NOD male kidney


text: Genoscope - Centre National de Sequencage

text: live

text: Generic

text: The cDNA library was constructed from total RNA, using the CloneMiner cDNA library construction kit (Invitrogen)

text: Paramecium tetraurelia

text: conjugation (beginning of meiosis)

text: Paramecium tetraurelia conjugation (beginning of meiosis)


text: live

text: Generic

text: Paramecium tetraurelia

text: Paramecium tetraurelia vegetative cells at standard temperature (27 degrees Celsius)


text: live

text: Generic

text: Paramecium tetraurelia

text: Paramecium tetraurelia conjugation (meiosis and beginning of macronuclear development)


text: live

text: Generic

text: Paramecium tetraurelia

text: autogamy (macronuclear development)

text: Paramecium tetraurelia autogamy (macronuclear development)


text: live

text: Generic

text: Paramecium tetraurelia

text: Paramecium tetraurelia vegetative cells at high temperature (39 degrees Celsius)


text: live

text: Generic

text: Paramecium tetraurelia

text: Paramecium tetraurelia vegetative cells at high temperature (35 degrees Celsius)


text: Centro de Investigacion y de Estudios Avanzados del IPN

text: live

text: Generic

text: Spores and mycelia

text: mRNA extracted from mycelia and spore cells was used to obtain cDNA, which was then cloned into lambda TriplEx2.

text: two months-old after germination

text: E. coli BM25.8

characteristic: lab-host

text: Rhizophagus intraradices

text: EcoRI

text: HindIII

text: asexual

text: germinating spore tissue

text: Glomus intraradices two months after germination

text: Plasmid


text: Beijing Institute of Genomics, Chinese Academy of Sciences

text: live

text: Generic

text: adult

text: eyeball

characteristic: organ

text: Cavia porcellus

text: cavy eyeball cDNA Library

text: plasmid

text: pBluescriptII

characteristic: vector


characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: adult

text: eyeball

text: Homo sapiens

text: human eyeball cDNA Library

text: plasmid

text: pBluescriptII


characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: adult

text: eyeball

text: Mus musculus

text: mouse eyeball cDNA Library

text: plasmid

text: pBluescriptII


text: Guangdong Pharmaceutical University

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: root

text: Glycyrrhiza uralensis

text: hermaphrodite

text: root

text: Glycyrrhiza uralensis root tissue


text: IAC, Instituto Agronomico de Campinas

text: live

text: Generic

characteristic: cultivar

text: The cDNA reverse subtractive library (SC) from peanut's leaves was gerated using Supression Subtractive Hibridization (SSH) methods (Diantchenko et al., 1996), as tester cDNA from peanut leaves and as driver cDNA from inoculated with Cercosporidium personatum pooled samples colleted at 12, 24 and 72 hour after inoculation.

characteristic: description

text: first and second opened leaves

text: leaves

text: Arachis hypogaea subsp. fastigiata

characteristic: organism

text: SC, cDNA reverse subtractive library from peanut leaves

text: Plasmid

text: pGEM T.


text: IAC, Instituto Agronomico de Campinas

text: live

text: Generic

text: The cDNA forward subtractive library (SI) from peanut's leaves inoculated with Cercosporidium personatum was gerated using Supression Subtractive Hibridization (SSH) methods (Diantchenko et al., 1996), as tester cDNA from peanut leaves pooled samples colleted at 12, 24 and 72 hour after inoculation with C. personatum, and as driver cDNA from control samples non-inoculated.

text: first and second opened leaves

characteristic: dev-stage

text: leaves

text: Arachis hypogaea subsp. fastigiata

text: SI, cDNA forward subtractive library from peanut leaves inoculated with Cercosporidium personatum

characteristic: title

text: Plasmid

text: pGEM T.


characteristic: External Id

text: Chiba University

text: live

text: Generic

text: synonym: Petunia hybrida

text: Petunia x hybrida

text: flower

text: full-length enriched petunia cDNA library


text: Osaka University

text: live

text: Generic

text: synonym: Petunia hybrida

text: Petunia axillaris subsp. axillaris

text: pollen

text: petunia pollen cDNA library


text: Chiba University

text: live

text: Generic

text: synonym: Petunia hybrida

text: Petunia axillaris subsp. axillaris

text: pollen tube

text: petunia pollen tube cDNA library


text: University of Chicago

text: live

text: Generic

text: Landsberg (ms-1 mutant)

text: Ovules were microdissected from stage14 flowers. 0.5 ug of RNA was reverse transcribed and PCR amplified for 15 cycles using the BD SMART protocol (Clontech). cDNAs were fragmented and sequenced by the 454 corporation.

text: Ovule

text: Arabidopsis thaliana

text: Arabidopsis ovule high throughput cDNA library


text: USDA, Agricultural Research Service

text: live

text: Generic

text: Canadian Harmony on Tennessee Natural rootstock

text: Total RNA was prepared from peach bark and used as a template for cDNA synthesis using the SuperSMART system (Clontech, Palo Alto, CA). Amplification of cDNAs and suppression subtractive hybridization (SSH) followed the manufacturer's protocol for SSH (Clontech). PCR-amplified products resulting from two rounds of hybridization were cloned non-directionally into pCR2.1 (Invitrogen, Carlsbad, CA). Colony PCR using M13 forward and reverse primers was used to identify recombinant plasmids containing inserts >200 bp. Recombinant plasmid DNA was sequenced in both directions using Nested PCR primers 1 and 2R (Clontech) or M13 forward and reverse primers. The sequences obtained from the A Reverse library (AR library) represent sequences upregulated at 5oC in nightbreak.

text: current year's growth

text: E. coli DH5alpha

text: branch

text: Prunus persica

text: monoecious

characteristic: sex

text: bark (mostly cambium)

text: Subtraction suppressive hybridization library A Reverse

text: plasmid


text: USDA, Agricultural Research Service

text: live

text: Generic

text: Total RNA was prepared from peach bark and used as a template for cDNA synthesis using the SuperSMART system (Clontech, Palo Alto, CA). Amplification of cDNAs and suppression subtractive hybridization (SSH) followed the manufacturer's protocol for SSH (Clontech). PCR-amplified products resulting from two rounds of hybridization were cloned non-directionally into pCR2.1 (Invitrogen, Carlsbad, CA). Colony PCR using M13 forward and reverse primers was used to identify recombinant plasmids containing inserts >200 bp. Recombinant plasmid DNA was sequenced in both directions using Nested PCR primers 1 and 2R (Clontech) or M13 forward and reverse primers. The sequences obtained from the A Forward library represent sequences downregulated at 5oC in nightbreak.

text: current year's growth

text: E. coli DH5alpha

text: branch

text: Prunus persica

text: monoecious

text: bark (mostly cambium)

characteristic: tissue-type

text: Subtraction suppressive hybridization library A Forward

text: plasmid


text: University of Padova

text: live

text: Generic

text: cDNA-AFLP transcript derived fragment

text: Hypericum perforatum

text: flower

text: Hypericum perforatum flower cDNA


text: Swedish University of Agricultural Sciences (SLU)

text: live

text: Generic

text: Westar

text: Brassica napus

text: Leaf

text: Brassica napus cv. Westar leaf cDNA-AFLP


text: University of Minnesota

text: live

text: Generic

text: heart

text: Meleagris gallopavo

text: cardiac muscle

text: plasmid


text: Michigan State University

text: live

text: Generic

text: Dreissena polymorpha

text: Dreissena polymorpha library (Xu W)


text: The University of Tokyo

text: live

text: Generic

text: adult

text: Capra hircus

text: skin

text: Capra hircus adult skin cDNA subtracted library


text: The University of Tokyo

text: live

text: Generic

text: Capra hircus

text: skin

text: Capra hircus skin cDNA subtracted library


text: College of Fisheries, Karnataka Veterinary, Animal and Fisheries Sciences University

text: live

text: Generic

text: Haemocyte

characteristic: cell type

text: Penaeus monodon

text: WSSV infected Penaeus monodon subtractive hybridization library

text: pSC-A


text: The Plant Biotechnology Institute, National Research Council Canada

text: live

text: Generic

text: Topas (embryogenic line DH4079)

text: Embryogenesis was induced in microspores isolated at the late-uninucleate to early-binucleate stage with a 3d heat stress treatment (32 degrees C). Sieving prior to RNA isolation removed the small non-induced microspores. These 3d cultures contained embryogenic as well as pollen-like structures.

text: 3d of 32 degrees C heat stress

text: Brassica napus

text: Sfi IA

text: Sfi IB

text: microspore

text: plasmid

text: pDNR-LIB


text: Cold Spring Harbor Laboratory

text: live

text: Generic

text: Stratagene ZAP Express cDNA Synthesis Kit. The library was size-fractionated to enrich for large inserts.

text: leaf

characteristic: organ

text: Ginkgo biloba

text: XhoI

text: Eco RI

text: female

text: Ginkgo female leaf (NYBG)

text: Plasmid

text: pBK-CMV


text: live

text: Generic

text: Topas (embryogenic line DH4079)

text: Embryogenesis was induced in microspores isolated at the late-uninucleate to early-binucleate stage with a 3d heat stress treatment (32 degrees C). Following heat stress, cultures were transferred to 24 degrees C for an additional 4d. These 7d cultures contained pre-globular and globular stage microspore-drived embryos.

text: 7d cultures; 3d of 32 degrees C heat stress, then 4d at 24 degrees C

text: Brassica napus

text: Sfi I A

text: Sfi I B

text: microspore

text: plasmid

text: pDNR-LIB

characteristic: vector


characteristic: INSDC center name

text: live

text: Generic

text: Topas (embryogenic line DH4079)

text: Embryogenesis was induced in microspores isolated at the late-uninucleate to early-binucleate stage with a 3d heat stress treatment (32 degrees C). Following heat stress, cultures were transferred to 24 degrees C for an additional 4d. These 7d cultures contained pre-globular and globular stage microspore-derived embryos.

text: Brassica napus

text: Eco RI

text: Xho I

text: microspore

text: plasmid

text: pBluescript II SK+


text: Harvard University

characteristic: INSDC first public

text: live

text: Generic

text: mRNA was isolated from muscle, skin, brain, kidney, gonad, juvenile and pharyngeal jaw of A. burtoni males and females. A cDNA library was generated with Clonetech smartseq technology and cloned into pAL 16, a 'TA' cloning vector. Duplex-specific nuclease4 (www.euregene.com) was used for library normalization.

text: mixed

text: XL1-Blue MRF' E.coli

characteristic: lab-host

text: Haplochromis burtoni

text: mixed

text: mixed (muscle, skin, brain, kidney, gonad, juvenile, phayngeal jaw)

text: Cichlid Pinky cDNA library 2003

text: plasmid


text: Boyce Thompson Institute for Plant Research

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: mixed instars

text: Myzus persicae

text: parthenogenetic female

text: Freeville 001

characteristic: strain

text: whole insect

text: Myzus persicae, line F001, PLRV free, whole female aphid library

text: plasmid

text: pDNR-LIB


text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: winged adult

text: Myzus persicae

text: parthenogenetic female

text: tobacco adapted lineage (red-USDA)

text: digestive tract

text: Myzus persicae, tobacco lineage, aphid digestive tract normalized library

text: plasmid

text: pDNR-LIB


text: live

text: Generic

text: mixed instars

text: Myzus persicae

characteristic: organism

text: parthenogenetic female

text: Geneva 006

text: whole insect

text: Myzus persicae, line G006, PLRV infected, whole female aphid library

text: plasmid

text: pDNR-LIB


text: live

text: Generic

text: mixed instars

characteristic: dev-stage

text: Myzus persicae

text: parthenogenetic female

text: Geneva 006

text: whole insect

text: Myzus persicae, line G006, PLRV free, whole female aphid library

text: plasmid

text: pDNR-LIB


text: live

text: Generic

text: winged adult

text: Myzus persicae

text: parthenogenetic female

text: tobacco-adapted lineage (red-USDA)

text: head

text: Myzus persicae, tobacco lineage, aphid head normalized library

characteristic: title

text: plasmid

text: pDNR-LIB


characteristic: External Id

text: Norwegian Forest Research Institute

text: live

text: Generic

text: Subtracted array: Tester: sample F6_12SD6 (cold environment); Driver: F6_11SD6 (warm environment) genes predominantly expressed in plants growing from seeds obtained after embryogenesis in cold environment (outdoor) after short day treatment (going to bud set); Suppressive subtraction hybridization was performed using the SuperSMART cDNA Synthesis Kit and the Clontech PCR-Select cDNA Subtraction Kit, Obtained arrays were size-fractionated using CHROMA SPIN-400 Columns and non-directionally cloned in a pDrive vector using PCR Cloning Kit.

characteristic: description

text: 1-year seedlings, growing in chambers with controlled conditions, on the 6th day after starting of short day treatment (12 hours of light and dark)

text: Picea abies

text: whole green part of seedlings

text: AA - Norway Spruce Subtracted Library (Adaptability-cold)

text: pDrive vector (Qiagen)


text: Norwegian Forest Research Institute

text: live

text: Generic

text: Subtracted array: Tester: sample F6_11SD6 (warm environment); Driver: F6_12SD6 (cold environment) genes predominantly expressed in plants growing from seeds obtained after embryogenesis in warm environment (in greenhouse) after short day treatment (going to bud set); Suppressive subtraction hybridization was performed using the SuperSMART cDNA Synthesis Kit and the Clontech PCR-Select cDNA Subtraction Kit, Obtained arrays were size-fractionated using CHROMA SPIN-400 Columns and non-directionally cloned in a pDrive vector using PCR Cloning Kit.

text: Picea abies

text: whole green part of seedlings

characteristic: tissue-type

text: BB - Norway Spruce Subtracted Library (Adaptability-warm)

text: pDrive vector (Qiagen)


text: Norwegian Forest Research Institute

text: live

text: Generic

text: Subtracted array: Tester: sample F6_12SD6 (short day, cold environment); Driver: F6_12LD6 (long day, cold environment - control) genes predominantly expressed in plants growing from seeds obtained after embryogenesis in cold environment (outdoor) after short day treatment (going to bud set); Suppressive subtraction hybridization was performed using the SuperSMART cDNA Synthesis Kit and the Clontech PCR-Select cDNA Subtraction Kit, Obtained arrays were size-fractionated using CHROMA SPIN-400 Columns and non-directionally cloned in a pDrive vector using PCR Cloning Kit.

text: Picea abies

text: whole green part of seedlings

text: CC - Norway Spruce Subtracted Library (bud set - short day)

text: pDrive vector (Qiagen)


text: Norwegian Forest Research Institute

text: live

text: Generic

text: Subtracted array: Tester: sample F6_12LD6 (long day, cold environment - control); Driver: F6_12SD6 (short day, cold environment) genes predominantly expressed in plants growing from seeds obtained after embryogenesis in cold environment (outdoor) without short day treatment (continue growing); Suppressive subtraction hybridization was performed using the SuperSMART cDNA Synthesis Kit and the Clontech PCR-Select cDNA Subtraction Kit, Obtained arrays were size-fractionated using CHROMA SPIN-400 Columns and non-directionally cloned in a pDrive vector using PCR Cloning Kit.

text: Picea abies

text: whole green part of seedlings

text: DD - Norway Spruce Subtracted Library (bud set - long day)

text: pDrive vector (Qiagen)


text: Shanghai University

text: live

text: Generic

characteristic: cultivar

text: Triticum aestivum inoculated with Puccinia striiformis f. sp. tritici; country: China: Gansu province, Tianshui district

text: Triticum aestivum

text: Triticum aestivum cv. 89-27 inoculated with Puccinia striiformis f. sp. tritici


text: Shanghai University

text: live

text: Generic

text: XianNong 4

text: Triticum aestivum

text: Triticum aestivum cv. XianNong 4 inoculated with Puccinia striiformis f. sp. tritici


text: NRC Institute for Marine Biosciences Industrial Partnership Facility

text: live

text: Generic

text: Gadus morhua

text: liver

text: Gadus morhua liver thermal stress library gmnblits


text: University of British Columbia

text: live

text: Generic

text: The top leaders of 5 mature trees were sprayed with a 0.01% (v/v) methyl jasmonate solution, resuspended in 0.1% (v/v) Tween 20 (~50 ml per tree). Bark tissue, with phloem attached, was harvested at 6 hrs, 1, 2, 4, 6 days after treatment initiation. Equamolar amounts of mRNA from each sampling time were pooled for library construction. cDNA was prepared from 5 micrograms of mRNA and directionally ligated into the pBluescript II SK+ vector using Stratagene's pBluescript II XR cDNA Library Construction kit according to manufacturer's instructions, with modifications. Plasmid DNA was then transformed by electroporation into DH10B cells (Invitrogen). Normalization was carried out according to published methods (Bonaldo M.F. et al. 1996, Genome Research 6: 791:806).

text: E. coli DH10B

text: Picea sitchensis

text: EcoRI (5' end of the cDNA)

text: XhoI (3' end of the cDNA)

text: hermaphrodite

characteristic: sex

text: Leader bark (phloem and cambium attached) treated with methyl jasmonate

text: Plasmid

text: pBluescript II SK+


text: University of British Columbia

text: live

text: Generic

text: The top leaders of 5 mature trees were wounded with a needle at 5 mm intervals on opposite sides of the leader. Bark tissue, with phloem attached, was harvested at 6 hrs, 1, 2, 4, 6 days after treatment initiation. Equamolar amounts of mRNA from each sampling time were pooled for library construction. cDNA was prepared from 5 micrograms of mRNA and directionally ligated into the pBluescript II SK+ vector using Stratagene's pBluescript II XR cDNA Library Construction kit according to manufacturer's instructions, with modifications. Plasmid DNA was then transformed by electroporation into DH10B cells (Invitrogen). Normalization was carried out according to published methods (Bonaldo M.F. et al. 1996, Genome Research 6: 791:806).

text: E. coli DH10B

text: Picea sitchensis

text: EcoRI (5' end of the cDNA)

text: XhoI (3' end of the cDNA)

text: hermaphrodite

text: Leader bark (phloem and cambium attached) with wounding treatment

text: Plasmid

text: pBluescript II SK+

characteristic: vector


text: University of British Columbia

characteristic: INSDC center name

text: live

text: Generic

text: The top leaders of 5 mature trees were sprayed with a 0.01% (v/v) methyl jasmonate solution, resuspended in 0.1% (v/v) Tween 20 (~50 ml per tree). Bark tissue, with phloem attached, was harvested at 6 hrs, 1, 2, 4, 6 days after treatment initiation. Equamolar amounts of mRNA from each sampling time were pooled for library construction. cDNA was prepared from 5 micrograms of mRNA and directionally ligated into the pBluescript II SK+ vector using Stratagene's pBluescript II XR cDNA Library Construction kit according to manufacturer's instructions, with modifications. Plasmid DNA was then transformed by electroporation into DH10B cells (Invitrogen).

text: E. coli DH10B

text: Picea sitchensis

text: EcoRI (5' end of the cDNA)

text: XhoI (3' end of the cDNA)

text: hermaphrodite

text: Plasmid

text: pBluescript II SK+


text: University of British Columbia

characteristic: INSDC first public

text: live

text: Generic

text: The top leaders of 5 mature trees were caged with 5 weevils (Pissodes strobi) each. Bark tissue, with phloem attached, was harvested at 6 hrs, 1, 2, 4, 6 days after continuous feeding. Equamolar amounts of mRNA from each sampling time were pooled for library construction. cDNA was prepared from 5 micrograms of mRNA and directionally ligated into the pBluescript II SK+ vector using Stratagene's pBluescript II XR cDNA Library Construction kit according to manufacturer's instructions, with modifications. Plasmid DNA was then transformed by electroporation into DH10B cells (Invitrogen).

text: E. coli DH10B

text: Picea sitchensis

text: EcoRI (5' end of the cDNA)

text: XhoI (3' end of the cDNA)

text: hermaphrodite

text: Leader bark (phloem and cambium attached) induced with Pissodes strobi (white pine weevil) feeding

text: Plasmid

text: pBluescript II SK+


text: University of British Columbia

text: live

text: Generic

text: New shoot growth was harvested from 5 mature trees. Equamolar amounts of mRNA from each sampling time were pooled for library construction. cDNA was prepared from 5 micrograms of mRNA and directionally ligated into the pBluescript II SK+ vector using Stratagene's pBluescript II XR cDNA Library Construction kit according to manufacturer's instructions, with modifications. Plasmid DNA was then transformed by electroporation into DH10B cells (Invitrogen).

text: E. coli DH10B

characteristic: lab-host

text: Picea sitchensis

text: EcoRI (5' end of the cDNA)

text: XhoI (3' end of the cDNA)

text: hermaphrodite

text: Shoot

text: Plasmid

text: pBluescript II SK+


text: Max-Planck-Institut fuer Molekulare Genetik

characteristic: INSDC last update

text: live

text: Generic

text: isolation_source: wildcaught; country: France:Villefranche

text: cleavage and early blastula stage

text: Paracentrotus lividus

text: whole embryo

text: RZPD MPMGp1171 Paracentrotus lividus CLEB


text: live

characteristic: INSDC status

text: Generic

text: gastrula and pluteus stage

text: Paracentrotus lividus

text: whole embryo

text: RZPD MPMGp1173 Paracentrotus lividus GASPLU


text: Huazhong Agricultural University

text: live

text: Generic

text: erythrocytic stage

text: Babesia orientalis

text: sfiIA

text: sfiIB

text: Wuhan-Hubei, China

characteristic: strain

text: cDNA library of Babesia orientalis Wuhan-Hubei

text: Phage


text: The Plant Biotechnology Institute, National Research Council Canada

text: live

text: Generic

characteristic: NCBI submission model

text: Topas (embryogenic line DH4079)

text: Embryogenesis was induced in the microspores isolated at the late-uninucleate to early-binucleate stage with a 3d heat stress treatment (32 degrees C). Following heat stress, cultures were transferred to 24 degrees C for an additional 2d. These 5d cultures contained embryogenic as well as some pollen-like structures.

text: 5d cultures; 3d of 32 degrees C heat stress, then 2d at 24 degrees C

text: Brassica napus

text: Sfi 1A

text: Sfi 1B

text: Microspore

text: Plasmid

text: pDNR-LIB


text: live

text: Generic

characteristic: NCBI submission package

text: Topas (embryogenic line DH4079)

text: Brassica napus

text: Eco RI

text: Xho I

text: Microspore

text: Plasmid

text: pBluescript II SK+


text: Corporacion para Investigaciones Biologicas

text: live

text: Generic

text: Conidia to Yeast Transition

text: Paracoccidioides brasiliensis

characteristic: organism

text: Conidia to yeast transition EST-Orestes Library


text: National Institute of Radiological Sciences

text: live

text: Generic

text: ES

characteristic: cell type

characteristic: cell-line

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5'-CAGCGGCCGCTTTTTTTTTTTTTTTTTTV-3']; double-stranded cDNA was ligated to Sal I adaptors [5'-TCGACCCACGCGTCCG-3'], digested with Not I and cloned into the Sal I and Not I sites of pSPORT 1 vector.; Average insert size: 0.7 kb (range 0.2-1.3 kb); Sequence primer: T7 [5'-TAATACGACTCACTATAGGG-3']; Primary library, non-amplified.

text: Mus musculus

text: Sal I

text: Not I

text: Abe mouse ES cell

text: pSPORT 1 (GibcoBRL)


characteristic: External Id

text: Washington University School of Medicine

text: live

text: Generic

text: Lucigen E.cloni 10G Supreme

text: Biomphalaria glabrata

text: HincII

text: HincII

characteristic: title

text: plasmid

text: pSMART


text: Okazaki National Research Institutes

text: live

text: Generic

text: Malaria parasites harboring GFP driven by the hsp70 promoter were used (see Ishino et al. Mol Microbiol. 2006;59(4):1175-84.). Maralia parasites (hsp70:GFP) infected hepatocytes were collected from female wistar rats by perfusion 31 h after sporozoites inoculation, and were isolated by cell-sorting. Total RNAs were extracted using RNAgents Total RNA Isolation System (Promega). Directional cDNA libraries were constructed into pDNR-LIB vector by PCR-based method using Creator Smart cDNA library Construction Kit (BD Bioscience).

characteristic: description

text: liver stage parasite

characteristic: dev-stage

text: Plasmodium berghei

text: Plasmodium berghei liver stage parasite

text: pDNR_LIB (Clontech)


text: Okazaki National Research Institutes

text: live

text: Generic

text: Plasmodium sporozoites were collected from mosquito salivary grands 25-30 days after infection. mRNAs were extracted using micro-mRNA extraction kit (Pharmacia). Directional cDNA libraries were constructed by oligo d(T) priming of poly(A)+ RNA into SalI and NotI sites of pSPORT 1 vector using the Superscript Plasmid System for cDNA Synthesis and Plasmid Cloning Kit (Life Technologies, Inc.)

text: salivary gland sporozoite

text: Plasmodium berghei ANKA

text: SalI

text: NotI

text: ANKA

text: Plasmodium berghei salivary grand sporozoite

text: pSPORT 1


text: Okazaki National Research Institutes

text: live

text: Generic

text: Plasmodium sporozoites were collected from mosquito midguts 21 days after infection. mRNAs were extracted using micro-mRNA extraction kit (Pharmacia). Directional cDNA libraries were constructed by oligo d(T) priming of poly(A)+ RNA into SalI and NotI sites of pSPORT 1 vector using the Superscript Plasmid System for cDNA Synthesis and Plasmid Cloning Kit (Life Technologies, Inc.)

text: midgut sporozoite

text: Plasmodium berghei ANKA

text: SalI

text: NotI

text: ANKA

text: Plasmodium berghei midgut sporozoite

text: pSPORT 1


text: Okazaki National Research Institutes

text: live

text: Generic

text: Schizonts were enriched by in vitro culture method using infected wistar rats blood, and purified with density gradient centrifugation using NycoPrep (1.077 A; AXIS-SHIELD PoC AS) mRNAs were extracted using micro-mRNA extraction kit (Pharmacia). Directional cDNA libraries were constructed by oligo d(T) priming of poly(A)+ RNA into SalI and NotI sites of pSPORT 1 vector using the Superscript Plasmid System for cDNA Synthesis and Plasmid Cloning Kit (Life Technologies, Inc.)

text: erythrocytic schizont

text: Plasmodium berghei ANKA

text: SalI

text: NotI

text: ANKA

text: Plasmodium berghei erythrocytic schizont

text: pSPORT 1


text: National Institute of Genetics

text: live

text: Generic

characteristic: cultivar

text: Triticum aestivum

text: root

characteristic: tissue-type

text: Y. Ogihara unpublished cDNA library, whsct


text: National Institute of Genetics

characteristic: INSDC center name

text: live

text: Generic

text: Triticum aestivum

text: root

text: Y. Ogihara unpublished cDNA library, whatlal


text: National Institute of Genetics

characteristic: INSDC first public

text: live

text: Generic

text: treated at 50 micro M Al for 6h

text: Triticum aestivum

text: root

text: Y. Ogihara unpublished cDNA library, whsctal


text: National Institute of Genetics

text: live

text: Generic

text: Thatcher Avr Lr10

text: infected with leaf rust

text: seedling

text: Triticum aestivum

text: Y. Ogihara unpublished cDNA library, whthls


text: National Institute of Genetics

text: live

text: Generic

text: Near isogenic line Thatcher Avr Lr10

text: infected with leaf rust

text: seedling

text: Triticum aestivum

text: Y. Ogihara unpublished cDNA library, whthkles


text: National Institute of Genetics

text: live

text: Generic

text: Chancellor AvrPm3b

text: seedling

text: Triticum aestivum

text: Y. Ogihara unpublished cDNA library, whchan


text: National Institute of Genetics

text: live

text: Generic

text: adult

text: Oryzias latipes

text: regenerating adult caudal fin, 3 days postamputation

text: Oryzias latipes regenerating fin 3 days postamputation cDNA library


text: National Institute of Genetics

text: live

text: Generic

text: Triticum aestivum

text: root

text: Y. Ogihara unpublished cDNA library, whatl


text: National Institute of Genetics

text: live

text: Generic

text: Near isogenic line AvrPm3b Chul/*8chan

text: infected with powdery mildew (AvrPm3b)

text: seedling

text: Triticum aestivum

text: Y. Ogihara unpublished cDNA library, whchul


text: National Institute of Genetics

text: live

text: Generic

text: adult

text: Oryzias latipes

text: regenerating adult caudal fin, 10 days postamputation

text: Oryzias latipes regenerating fin 10 days postamputation cDNA library


text: National Institute of Fruit Tree Science

text: live

text: Generic

text: Tsugaru

text: Malus domestica

text: mature fruit skin

text: Malus x domestica cv. Tsugaru mature fruit skin


text: live

text: Generic

text: Malus domestica

text: mature fruit skin

text: Malus x domestica mature fruit skin


text: live

text: Generic

text: Ballad

text: Pyrus communis

text: Pyrus communis cv. Ballad


text: Washington University School of Medicine

text: live

text: Generic

text: The library was constructed using mRNA isolated from total RNA with oligo-dT cellulose. Total RNA was generated from muscle larvae that were isolated from infected rats. Larvae were liberated by pepsin/HCl digestion, incubated with 1% SDS, treated with RNase and DNase to eliminate host nucleic acid contamination, and purified on a Percoll gradient.

text: Muscle larvae

text: DH10B cells

characteristic: lab-host

text: Trichinella spiralis

text: NotI

text: SalI

text: plasmid

characteristic: vector


text: National Institute of Agrobiological Sciences

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: main and accessory lymphocyte

text: 5 weeks

text: Sus scrofa

text: male

characteristic: sex

text: full-length enriched pig cDNA library, main and accessory lymphocyte


text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: 5 weeks

text: Sus scrofa

text: male

text: main and accessory hippocampus

text: full-length enriched pig cDNA library, main and accessory hippocampus


text: live

text: Generic

text: 5 weeks

text: Sus scrofa

characteristic: organism

text: male

text: main and accessory dentate gyrus

text: full-length enriched pig cDNA library, main and accessory dentate gyrus


text: live

text: Generic

text: 5 weeks

text: Sus scrofa

text: male

text: main and accessory cerebral cortex

text: full-length enriched pig cDNA library, main and accessory cerebral cortex


text: live

text: Generic

text: 5 weeks

text: Sus scrofa

text: male

text: main and accessory basal ganglia

text: full-length enriched pig cDNA library, main and accessory basal ganglia


text: Chiba Cancer Center Research Institute

text: live

text: Generic

text: Homo sapiens

text: hepatoblastoma

text: human hepatoblastoma cDNA


text: Chiba Cancer Center Research Institute

text: live

text: Generic

text: Homo sapiens

text: Wilms tumor

text: Human Wilms tumor cDNA


text: live

text: Generic

text: Caturra

text: The cDNA library was constructed from mRNA isolated from young coffee leaves. Complementary DNA was synthesized from mRNA using the primer oligo-dT/CDSIII (3' priming) and smart oligonucleotide that attaches at the 5' end during elongation with reverse transcriptase. The library is non-directional. Cycle sequencing of individual clones was performed and the samples run on an ABI377 sequencing machine. The project was a collaborative research initiative between the Colombian National Center for Coffee Research - CENICAFE and the laboratory directed by Dr. Susan McCouch, Department of Plant Breeding, Cornell University, Ithaca, NY. This research was partially financed by COLCIENCIAS-Instituto Colombiano para el Desarrollo de la Ciencia

text: Leaf

characteristic: organ

text: Coffea arabica

text: Coffee leaves PCRScript library

text: PCRScript


characteristic: External Id

text: RIKEN Center for Developmental Biology

text: live

text: Generic

text: country: Japan

text: adult

text: Cynops pyrrhogaster

text: limb

text: Cynops pyrrhogaster (newt) adult limb cDNA library


text: USDA-ARS-Northern Crop Science Laboratory

text: live

text: Generic

text: Aphanomyces cochlioides

characteristic: strain

text: mycelium in water on sterile beet slices

text: Aph cDNA-BeetSlice

characteristic: title


text: live

text: Generic

text: Aphanomyces cochlioides

text: mycelium in potato dextrose broth

text: Aph cDNA-PDB


text: live

text: Generic

text: Aphanomyces cochlioides

text: infected sugarbeet seedlings

text: Aph cDNA-InfSeedling


text: University of Fribourg

text: live

text: Generic

text: mycelium

characteristic: cell type

text: HH

characteristic: isolate

text: Phytophthora brassicae

text: mycelium

text: non-sporulating culture of P. brassicae


text: University of Fribourg

text: live

text: Generic

text: mycelium

text: HH

text: Phytophthora brassicae

text: mycelium

characteristic: tissue-type

text: carbon starvation culture of P. brassicae


text: University of Fribourg

characteristic: INSDC center name

text: live

text: Generic

text: zoospore

text: HH

text: Phytophthora brassicae

text: zoospore

text: germinating cysts of P. brassicae


text: University of Fribourg

characteristic: INSDC first public

text: live

text: Generic

text: mycelium

text: HH

text: Phytophthora brassicae

text: mycelium

text: in vitro interaction, P. brassicae


text: University of Fribourg

text: live

text: Generic

text: mycelium

text: HH

text: Phytophthora brassicae

text: mycelium

text: nitrogen starvation culture of P. brassicae


text: College of Veterinary Medicine, South China Agricultural University

text: live

text: Generic

text: The library was constructed by a suppressive-substractive hybridization approach

characteristic: description

text: third stage larvae

characteristic: dev-stage

text: Ascaris suum

text: infective larvae of Ascaris suum


text: Genoscope - Centre National de Sequencage

characteristic: INSDC last update

text: live

text: Generic

text: The cDNA library was constructed from total RNA using the CloneMiner cDNA library construction kit (Invitrogen)

text: Phaeodactylum tricornutum

text: Phaeodactylum tricornutum low decadienal treated (0.5 ug/ml 2E, 4E-decadienal for 6 hours)


text: live

characteristic: INSDC status

text: Generic

text: Phaeodactylum tricornutum

text: iron starved (5nM of iron)

text: Phaeodactylum tricornutum iron starved (5nM of iron)


text: live

text: Generic

text: Phaeodactylum tricornutum

text: Phaeodactylum tricornutum silica plus (350 uM metasilicate in artificial seawater)


text: live

text: Generic

text: Phaeodactylum tricornutum

text: Phaeodactylum tricornutum tropical acession Pt9 (cold stress; grown at 15 degrees celsius)


text: live

text: Generic

text: Phaeodactylum tricornutum

text: oval morphology (low salinity, 10% SW)

text: Phaeodactylum tricornutum oval morphology (low salinity, 10% SW) Pt3(CCAP1052/1B)


text: live

text: Generic

text: Phaeodactylum tricornutum

text: Phaeodactylum tricornutum nitrate starved (50 uM for 3 days in chemostat culture)


text: live

text: Generic

text: Phaeodactylum tricornutum

text: ammonium adapted (75uM of ammonium)

text: Phaeodactylum tricornutum ammonium adapted (75uM of ammonium)


text: live

text: Generic

text: Phaeodactylum tricornutum

text: silica minus (artificial seawater)

text: Phaeodactylum tricornutum silica minus (artificial seawater)


text: live

text: Generic

text: Phaeodactylum tricornutum

text: Phaeodactylum tricornutum nitrate repleted (1.12 mM chemostat culture)


text: live

text: Generic

text: Phaeodactylum tricornutum

text: Phaeodactylum tricornutum high decadienal treated (5 ug/ml 2E, 4E-decadienal for 6 hours)


text: live

text: Generic

text: Phaeodactylum tricornutum

text: urea adapted (50uM of urea)

text: Phaeodactylum tricornutum urea adapted (50uM of urea)


text: NewBioTechnic, S.A. (NBT)

text: live

text: Generic

text: Trichoderma asperellum

text: CECT 20268

text: mycelium Lambda Zap Library L14


text: NewBioTechnic, S.A. (NBT)

text: live

text: Generic

text: Trichoderma longibrachiatum

text: NBT T52

text: mycelium Lambda Zap Library L19


text: University of Toronto

text: live

text: Generic

text: Total RNA was isolated from pooled brain tissues using modified Trizol method (Invitrogen), then mRNA was extracted and purified from total RNA using Oligotex mRNA kit (Qiagen). cDNA was prepared from 5 micrograms of mRNA and directionally ligated into the pBluescript II SK (Sfi I) vector using SMARTTM cDNA library construction kit according to manufacturer's instructions (Clontech). Plasmid DNA was then transformed by electroporation into DH10B cells (Invitrogen) for propagation. Normalization was applied according to published methods [Bonaldo M.F. et al. (1996) Genome Research 6(9):791] in order to reduce the abundance of highly expressed transcripts.

text: adult

characteristic: lab-host

text: brain

text: Lymnaea stagnalis

text: EcoRI (5' end)

text: XhoI (3' end)

text: hermaphrodite

characteristic: sex

text: Whole ganglia ring was isolated from animals before and after a CNS regeneration procedure

text: pBluescript II SK (SfiI)

characteristic: vector


text: University of Georgia

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Genotype T89

characteristic: cultivar

text: Cynodon dactylon

characteristic: organism

text: Bermudagrass Normalized cDNA Library

text: Clontech's pTriplEx2


characteristic: External Id

text: Kyungpook National University School of Medicine

text: live

text: Generic

text: dermal papilla

text: Library was constructed using ZAP-cDNA synthesis kit (Stratagene). mRNA was prepared from cultured dermal papilla cells which isolated from beard. Cloned unidirectionally. Primer: Oligo dT. Average insert size: 1.5 kb. Uni-ZAP XR vector: 5' adaptor sequence, 5'-GAATTCGGCACGAG-3'; 3' adaptor sequence, 5'-CTCGAGTTTTTTTTTTTTTTTTTT-3'. Size fractionated to remove sequence <500 bp in size. Amplified once.

text: Primary culture

text: SOLR

text: skin

characteristic: organ

text: Homo sapiens

text: EcoRI

text: XhoI

text: beard hair

text: Human cultured dermal papilla cell cDNA library(B)

characteristic: title

text: plasmid

text: pBluescript SK-


text: live

text: Generic

text: dermal papilla

characteristic: cell type

text: Library was constructed using ZAP-cDNA synthesis kit (Stratagene). mRNA was prepared from cultured dermal papilla cells which isolated from occipital hair of hypotrichosis pubis patient of a woman. Cloned unidirectionally. Primer: Oligo dT. Average insert size: 1.5 kb. Uni-ZAP XR vector: 5' adaptor sequence, 5'-GAATTCGGCACGAG-3'; 3' adaptor sequence, 5'-CTCGAGTTTTTTTTTTTTTTTTTT-3'. Size fractionated to remove sequence <500 bp in size. Amplified once.

text: Primary culture

text: SOLR

text: skin

text: Homo sapiens

text: EcoRI

text: XhoI

text: occipital hair of pubic hypotrichosis patient

characteristic: tissue-type

text: Human cultured dermal papilla cell cDNA library(F)

text: plasmid

text: pBluescript SK-


characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: fibroblast

text: Library was constructed using ZAP-cDNA synthesis kit (Stratagene). mRNA was prepared from cultured fibroblasts which isolated from scalp interfollicular dermis. Cloned unidirectionally. Primer: Oligo dT. Average insert size: 1.5 kb. Uni-ZAP XR vector: 5' adaptor sequence, 5'-GAATTCGGCACGAG-3'; 3' adaptor sequence, 5'-CTCGAGTTTTTTTTTTTTTTTTTT-3'. Size fractionated to remove sequence <500 bp in size. Amplified once.

text: Primary culture

text: SOLR

text: skin

text: Homo sapiens

text: EcoRI

text: XhoI

text: scalp interfollicular dermis

text: Human cultured dermal papilla cell cDNA library(A)

text: plasmid

text: pBluescript SK-


text: live

text: Generic

text: dermal papilla

text: Library was constructed using ZAP-cDNA synthesis kit (Stratagene). mRNA was prepared from cultured dermal papilla cells which isolated from balding frontal hair. Cloned unidirectionally. Primer: Oligo dT. Average insert size: 1.5 kb. Uni-ZAP XR vector: 5' adaptor sequence, 5'-GAATTCGGCACGAG-3'; 3' adaptor sequence, 5'-CTCGAGTTTTTTTTTTTTTTTTTT-3'. Size fractionated to remove sequence <500 bp in size. Amplified once.

characteristic: description

text: Primary culture

characteristic: dev-stage

text: SOLR

text: skin

text: Homo sapiens

text: EcoRI

text: XhoI

text: frontal balding hair

text: Human cultured dermal papilla cell cDNA library(K)

text: plasmid

text: pBluescript SK-


characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: dermal papilla

text: Primary culture

text: SOLR

text: skin

text: Homo sapiens

text: EcoRI

text: XhoI

text: beard hair

text: Human cultured dermal papilla cell cDNA library(M)

text: plasmid

text: pBluescript SK-


text: live

text: Generic

text: dermal papilla

text: Library was constructed using ZAP-cDNA synthesis kit (Stratagene). mRNA was prepared from cultured dermal papilla cells which isolated from occipital hair of balding patient. Cloned unidirectionally. Primer: Oligo dT. Average insert size: 1.5 kb. Uni-ZAP XR vector: 5' adaptor sequence, 5'-GAATTCGGCACGAG-3'; 3' adaptor sequence, 5'-CTCGAGTTTTTTTTTTTTTTTTTT-3'. Size fractionated to remove sequence <500 bp in size. Amplified once.

text: Primary culture

text: SOLR

characteristic: lab-host

text: skin

text: Homo sapiens

text: EcoRI

text: XhoI

text: occipital scalp hair

text: Human cultured dermal papilla cell cDNA library(P)

text: plasmid

text: pBluescript SK-


text: live

text: Generic

text: Brassica napus

text: leaves

text: Brassica napus leaves cDNA-AFLP transcript-derived fragment


text: Faculty of Medicine of Ribeir_o Preto, University of S_o Paulo

text: live

text: Generic

text: melanoma

characteristic: cell-line

text: Suppression subtractive library generated using cDNA from a Radial Growth Phase melanoma cell line subtracted from cDNA from a pool of four metastatic melanoma cell lines.

text: Homo sapiens

text: EcoR V

text: melanoma cell line

text: Radial Growth Phase Melanoma SSH Library (RGP Library)

text: plasmid, TA-vector

text: pGEM-T Easy

characteristic: vector


text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: metastatic melanoma cell lines

text: WM9, WM852, WM1617, 1205Lu

text: Suppression subtractive library generated using cDNA from a pool of four metastatic melanoma cell lines subtracted from a radial growth phase melanoma cell line cDNA.

text: Homo sapiens

text: EcoR V

text: pool of melanoma cell lines

text: Metastatic Melanoma SSH Library (Met Library)

text: plasmid, TA-vector

text: pGEM-T Easy


text: Southwest Forestry University

text: live

text: Generic

text: Dendrocalamus sinicus

characteristic: organism

text: Dendrocalamus sinicus mRNA


characteristic: External Id

text: University of Florida

text: live

text: Generic

text: Alligator liver cDNA generated by random and oligo(dT) priming was cloned into the EcoRI site of pBluescript II SK. The cloning was not directional. Mean insert size estimated to be 0.9 kb with an approximate range of 0.3 to 1.4 kb.

text: Liver

text: Alligator mississippiensis

text: Male

characteristic: sex

text: Adult American Alligator Liver Library (Univ Florida Zoology)


text: University of Florida

text: live

text: Generic

text: Alligator testis cDNA generated by random and oligo(dT) priming was cloned into the EcoRI site of pBluescript II SK. The cloning was not directional. Mean insert size estimated to be 1.4 kb with an approximate range of 0.6 to 2.1 kb.

text: Testis

characteristic: organ

text: Alligator mississippiensis

text: Male

text: Adult American Alligator Testis Library (Univ Florida Zoology)

characteristic: title


text: University of Florida

text: live

text: Generic

text: Alligator liver cDNA generated by random and oligo(dT) priming. The cloning was not directional.

text: Liver

text: Alligator mississippiensis

text: Female

text: Juvenile American Alligator Liver Library (NIBB, Japan)


text: College of Plant Protection, Northwest A&F University

text: live

text: Generic

text: cDNA library constructed from germinated urediospores of Puccinia striiformis f.sp tritici by using SMART cDNA Library Construction Kit (Clontech, USA) following the manufacturer's instructions

text: Uredial stage

text: Wheat

text: Puccinia striiformis f. sp. tritici

text: SfiIAA and B

text: Chinese Race CY32

characteristic: strain

text: Wheat stripe rust fungus cDNA library

text: Phage

text: Lambda TriplEx2


text: Universidad Peruana Cayetano Heredia

characteristic: INSDC center name

text: live

text: Generic

text: country: Peru

characteristic: isolate

text: Leishmania braziliensis

text: Leishmania braziliensis amastigote stage, 72 hours post infection


text: Universidad Peruana Cayetano Heredia

characteristic: INSDC first public

text: live

text: Generic

text: country: Peru

characteristic: description

text: Leishmania braziliensis

text: Leishmania braziliensis procyclic stage, logarithmic growth phase


text: Western Kentucky University

characteristic: INSDC last update

text: live

text: Generic

text: 14-day-mercury treated seedlings

characteristic: dev-stage

text: Seedlings

text: Sesbania drummondii

text: bi-sexual

text: Shoots and roots

characteristic: tissue-type

text: Sesbania drummondii (Heavy metal hyperaccumulator) Suppression subtractive cDNA library


text: Institute of Himalayan Bioresource Technology, Palampur

text: live

characteristic: INSDC status

text: Generic

characteristic: cultivar

text: Camellia sinensis

text: Leaf

text: Camellia sinensis UPASI-10 suppression subtraction cDNA library

text: Plasmid

text: TA cloning vector


text: live

text: Generic

characteristic: NCBI submission model

text: Arabidopsis thaliana

text: leaf tissue

text: Arabidopsis thaliana col-0 suppression subtraction cDNA library

text: Plasmid

text: TA cloning vector

characteristic: vector


text: The Plant Biotechnology Institute, National Research Council Canada

text: live

text: Generic

characteristic: NCBI submission package

text: Topas (embryogenic line DH4079)

text: Subtractive library (Clontech PCR-Select) of freshly-isolated late-uninucleate and early-binucleate microspores (tester) compared to 3d heat-stress induced microspores (driver).

text: Freshly-isolated late-uninucleate and early-binucleate microspores.

text: Brassica napus

characteristic: organism

text: TA cloning bt. EcoRI and SpeI

text: TA cloning bt. EcoRI and SpeI

text: Microspore

text: Plasmid

text: pGEM-T Easy


characteristic: External Id

text: live

text: Generic

text: Topas (embryogenic line DH4079)

text: Subtractive library (Clontech PCR-Select) of 3d heat-stress induced microspores (tester) compared to freshly-isolated late-uninucleate and early-binucleate microspores (driver).

text: 3d of 32 degrees C heat stress

text: Brassica napus

text: TA cloning bt. EcoRI and SpeI

text: TA cloning bt. EcoRI and SpeI

text: Microspore

text: Plasmid

text: pGEM-T Easy


text: live

text: Generic

text: Topas (embryogenic line DH4079)

text: Subtractive library (Clontech PCR-Select) of 5d heat-stress induced microspores (3d heat stress at 32 degrees C, 2d at 24 degrees C) (tester) compared to freshly-isolated late-uninucleate and early-binucleate microspores and in vitro pollen (combined driver).

text: 5d; 3d of 32 degrees C heat stress, 2d at 24 degrees C

text: Brassica napus

text: TA cloning bt. EcoRI and SpeI

text: TA cloning bt. EcoRI and SpeI

text: Microspore

text: Plasmid

text: pGEM-T Easy


text: Stanford Human Genome Center

text: live

text: Generic

text: Vector: Express 1; The cDNA library was constructed from O.lucimarinus strain (CCE9901) isolated by B. Palenik from 32,9000 N 117,2550 W (Scripps Institution of Oceanography Pier, La Jolla, California, USA) and was grown as reported previously (Worden, A.Z, Nolan, J.K., and Palenik, B (2004) Limnology and Oceanography 49 , 168-179). Total and poly A+ RNA was isolated and a cDNA library was constructed in the Express 1 plasmid vector by Open Biosystems. First strand cDNA synthesis was primed with an 54 bp linker primer containing an oligodT sequence preceeded by a synthetic NotI site (first strand primer: 5'-GACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3'). Following second strand synthesis, cDNAs were made blunt at the end corresponding to the original 5 prime end of mRNA, and cloned directionally into the NotI and EcoRV sites of Express 1. Note that the EcoRV site is typically destroyed in the blunt end cloning, leaving a junction of the form 'xxxATC'(where is ATC is the second half of the EcoRV site, and xxx is derived from the cDNA sequence). A map of the Express 1 vector is available at: http://www.openbiosystems.com/cdna_library_construction_faq.php#8 The primary library was transformed and amplified in DH10B (T1 phage resistant) bacteria.

text: DH10B (T1 phage resistant)

characteristic: lab-host

text: Ostreococcus lucimarinus CCE9901

text: NotI

text: EcoRV

text: Whole organism

text: SHGC-CTZ

text: Plasmid

text: pExpress


text: Advanced Center for Genome Technology, University of Oklahoma

text: live

text: Generic

text: E.coli

text: Bos taurus

text: Not I

text: Sal I

text: Subcutaneous adipose tissue

text: cattle subcutaneous adipose tissue cDNA library

characteristic: title

text: Plasmid

text: pSPORT 1


text: Beijing University of Traditional Chinese Medicine

text: live

text: Generic

text: dormancy

text: Glycyrrhiza uralensis

text: NotI

text: EcoRI

text: root

text: Glycyrrhiza uralensis 1 year-old root tissue library

text: Plasmid

text: pBluescript II SK+


text: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences

text: live

text: Generic

text: Sporulated oocysts were obtained from cecea of E.tenella infected chickens.sporulated oocysts subtracted cDNA library using cDNA from sporulated oocysts cDNA as testers and cDNA from unsporulated oocysts as drivers. Subtractive PCRs were performed using the PCR-select cDNA subtractive kit according to the manufacture's protocol (Clontech). PCR products then had been directionally subcloned into the pGEM-T Easy vector (Promega) and transformed into JM109 competent cells (Promega).

text: Sporulated oocysts

text: Eimeria tenella

characteristic: strain

text: Eimeria tenella sporulated oocysts subtracted cDNA library

text: pGEM-T Easy


text: National Institute of Allergy and Infectious Diseases

characteristic: INSDC center name

text: live

text: Generic

text: Midgut

characteristic: cell type

text: PCR-based cDNA library made with SMART cDNA library construction kit from Clontech

text: Adult blood fed to repletion

text: Midgut

characteristic: organ

text: Phlebotomus papatasi

text: Female

characteristic: sex

text: Saudi Arabia

text: Midgut

text: P. papatasi adult female blood fed midgut library

text: Phage

text: Lambda TriplEx2


characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Midgut

characteristic: description

text: Adult blood fed to repletion

text: Midgut

text: Phlebotomus papatasi

text: Female

text: Saudi Arabia

text: Midgut

text: P. papatasi adult female blood fed with L. major midgut library

text: Phage

text: Lambda TriplEx2


text: live

characteristic: INSDC status

text: Generic

text: Midgut

text: Adult allowed only sucrose for feeding

characteristic: dev-stage

text: Midgut

text: Phlebotomus papatasi

text: Female

text: Saudi Arabia

text: Midgut

characteristic: tissue-type

text: P. papatasi adult female unfed/sugar fed midgut library

text: Phage

text: Lambda TriplEx2


text: Guangxi University

text: live

text: Generic

characteristic: NCBI submission model

text: Mycellium

text: Cryphonectria parasitica

text: Mycellium

text: Normalized cDNA library of mix of Cryphonectria parasitica (strain EP155 and EP155 infected with hypovirus CHV1-EP713)

characteristic: vector


text: Guangxi University

text: live

text: Generic

characteristic: NCBI submission package

text: Mycellium

text: Cryphonectria parasitica

characteristic: organism

text: Mycellium

text: Normalized mixed Cryphonectria parasitica cDNA library


characteristic: External Id

text: Guangxi University

text: live

text: Generic

text: Mycellium, conidium

text: Cryphonectria parasitica

text: Mycellium, conidium

text: Normalized cDNA library of Cryphonectria parasitica EP155

text: pBluescript SK(+)


text: Guangxi University

text: live

text: Generic

text: Mycellium, conidium

text: Cryphonectria parasitica

text: Mycellium, conidium

text: Normalized cDNA library of Cryphonectria parasitica EP155 infected with hypovirus CHV1-EP713

characteristic: title

text: pBluescript SK(+)


text: MPI for Developmental Biology

text: live

text: Generic

text: directional cloning

text: Embryo

text: E.coli

characteristic: lab-host

text: Embryo

text: Poecilia reticulata

text: Tranquille

characteristic: strain

text: Guppy pDNR_LIB Tranquille Embryo

text: Plasmid


text: MPI for Developmental Biology

characteristic: INSDC center name

text: live

text: Generic

text: directional cloning

text: Adult

text: E.coli

text: Liver

text: Poecilia reticulata

text: Tranquille

text: Guppy pDNR_LIB Tranquille Liver

text: Plasmid


text: MPI for Developmental Biology

text: live

text: Generic

text: directional cloning

text: Embryo

text: E.coli

text: Embryo

characteristic: organ

text: Poecilia reticulata

text: Quare

text: Guppy pDNR_LIB Quare Embryo

text: Plasmid


text: MPI for Developmental Biology

text: live

text: Generic

text: directional cloning

text: Adult

text: E.coli

text: Testis

text: Poecilia reticulata

text: Male

characteristic: sex

text: Quare

text: Guppy pDNR_LIB Quare Testis

text: Plasmid


text: MPI for Developmental Biology

characteristic: INSDC first public

text: live

text: Generic

text: directional cloning

text: Adult

text: E.coli

text: Head

text: Poecilia reticulata

text: Quare

text: Guppy pDNR_LIB Quare Head

text: Plasmid


text: MPI for Developmental Biology

characteristic: INSDC last update

text: live

text: Generic

text: directional cloning

text: Adult

text: E.coli

text: Liver

text: Poecilia reticulata

text: Quare

text: Guppy pDNR_LIB Quare Liver

text: Plasmid


text: MPI for Developmental Biology

text: live

text: Generic

text: directional cloning

characteristic: description

text: Adult

text: E.coli

text: Skin

text: Poecilia reticulata

text: Oropuche

text: Guppy pDNR_LIB Oropuche Skin

text: Plasmid


text: MPI for Developmental Biology

text: live

characteristic: INSDC status

text: Generic

text: directional cloning

text: Adult

text: E.coli

text: Retina

text: Poecilia reticulata

text: Oropuche

text: Guppy pDNR_LIB Oropuche Retina

text: Plasmid


text: MPI for Developmental Biology

text: live

text: Generic

text: directional cloning

text: Embryo

characteristic: dev-stage

text: E.coli

text: Embryo

text: Poecilia reticulata

text: Oropuche

text: Guppy pDNR_LIB Oropuche Embryo

text: Plasmid


text: MPI for Developmental Biology

text: live

text: Generic

text: directional cloning

text: Adult

text: E.coli

text: Testis

text: Poecilia reticulata

text: Male

text: Blue

text: Guppy pDNR_LIB Blue Testis

text: Plasmid


text: MPI for Developmental Biology

text: live

text: Generic

text: directional cloning

text: Adult

text: E.coli

text: Brain

text: Poecilia reticulata

text: Blue

text: Guppy pDNR_LIB Blue Brain

text: Plasmid


text: MPI for Developmental Biology

text: live

text: Generic

text: directional cloning

text: Adult

text: E.coli

text: Skin

text: Poecilia reticulata

text: Istanbul wild

text: Guppy pDNR_LIB Istanbul wild Skin

text: Plasmid


text: Tropical Medicine Institute

text: live

text: Generic

text: Artemisia annua

text: Artemisia annua library (Zhao C)


text: Stanford University School of Medicine

text: live

text: Generic

text: mRNA was prepared from a collection of California mussels that had been exposed to a variety of environmental challenges that included heat, cold, hypoxia, emersion, heavy metal, and salinity stress. The cDNA library was constructed using Cap-trapping methodology developed by RIKEN. Cap-trapped 1st strand cDNA was produced and hybridized with several biotinylated RNA drivers including: starting mRNA as the normalizing driver, and run-off transcripts from mini-libraries containing highly expressed genes, and previously sequenced cDNAs as subtracting drivers. cDNA that did not hybridize to the biotinylated driver was isolated, directionally-cloned into lambda FLC-I vector (from RIKEN), and plasmids excised using an in vivo liquid excision method.

text: DH10B (T1 phage resistant)

text: Mytilus californianus

text: GGCCAAATCGGCC

text: GGCCCTTATGGCC

text: Gill and adductor muscle

characteristic: tissue-type

text: SHGC-MUS

text: Plasmid

text: pFLC-I