text: Graduate School of Science, University of Tokyo; CREST, Japan Science and Technology Corporation, Japan

characteristic: INSDC last update

text: live

text: Generic

text: stage 12.5/13

text: Xenopus laevis

text: anterior endomesoderm

text: AEM cDNA library (lambda-ZAPII)


status: PUBLIC

characteristic: External Id

text: Thrombosis Research Institute

characteristic: INSDC center name

characteristic: INSDC first public

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: adult

tag: attribute

characteristic: dev-stage

text: Mus musculus

characteristic: organism

text: male

characteristic: sex

characteristic: strain

text: aortic arch

characteristic: tissue-type

text: Mus musculus aortic arch C57BL6 adult

characteristic: title


text: live

text: Generic

text: country=Turkey

characteristic: description

text: encysted zoospore

text: Saprolegnia parasitica

text: Spt

text: Saprolegnia parasitica Spt encysted zoospore


text: live

text: Generic

text: country=Turkey

text: mycelium

text: Saprolegnia parasitica

text: Spt

text: Saprolegnia parasitica Spt mycelium


text: Faculty of Veterinary Medicine

text: live

text: Generic

text: country=Belgium

text: adult

text: Ostertagia ostertagi

text: Ostertagia ostertagi adult


text: Faculty of Veterinary Medicine

text: live

text: Generic

text: Ostertagia ostertagi

text: Ostertagia ostertagi L3 (De Maere V)


text: University Medical Center St. Radboud

text: live

text: Generic

text: melanoma

characteristic: cell type

characteristic: cell-line

text: Homo sapiens

text: Homo sapiens 1F6


text: University of Delhi South Campus

text: live

text: Generic

text: Oryza sativa

text: Oryza sativa PCR-based subtractive cloning


text: Chungbuk National University

text: live

text: Generic

text: embryo

text: Eleutherococcus senticosus

text: callus

text: Eleutherococcus senticosus callus embryo


text: Vavilov Institute of General Genetics

text: live

text: Generic

text: adult

text: Rattus norvegicus

text: liver

text: Rat adult liver mRNA


text: Life Science College of Sichuan University and the department of anatomy of basic and legal medical institute of west china medical center of Sichuan University

text: live

text: Generic

text: 73 years old

text: Homo sapiens

text: rectum

text: cDNA subtractive library of human rectum adenocarcinoma

text: Plasmid

characteristic: vector


text: Carlsberg Laboratory

characteristic: INSDC last update

text: live

text: Generic

text: Blumeria hordei

text: Appressorium forming conidia

text: Appressorium stage EST library of Blumeria graminis f.sp. hordei

text: pBluescript II XR, Stratagene


characteristic: External Id

text: Institute of Hygiene and Environment Medicine

text: live

text: Generic

text: cold acclimatized

text: Mus musculus

text: BALB/c

text: muscle

text: Mus musculus muscle BALB/c cold acclimatized


text: University of Illinois at Urbana-Champaign

characteristic: INSDC center name

text: live

text: Generic

text: This BS cDNA library was generated by subtraction of the original non-normalized bovine spleen library with 16,800 previously sequenced clones from a bovine Placenta cDNA library. The original library was constructed as described by Band et al (2000), Genome Research 10(9): 1359 -1368.

text: Adult

text: Spleen

characteristic: organ

text: Bos taurus

text: EcoRI

text: XhoI

text: female

text: Angus

text: Subtracted Lewin Cattle Spleen

text: Phage

text: pBluescript SK(+/-)


text: Scottish Crop Research Institute

characteristic: INSDC first public

text: live

characteristic: INSDC status

text: Generic

text: Optic

characteristic: cultivar

text: Non-normalised library, directionally cloned into pSPORT1. Derived from endosperm tissue dissected from developing grains (6 days post anthesis) in glasshouse grown barley plants. Developed as part of the barley transcriptome resources of BBSRC/SEERAD funded cereal IGF (Investigating Gene Function) project.

text: 6 DPA

characteristic: lab-host

text: Hordeum vulgare subsp. vulgare

text: Sal I

text: Not I

text: endosperm

text: endosperm, 6 DPA, no treatment, cv Optic, EBed01

text: Plasmid


text: Scottish Crop Research Institute

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Optic

text: Non-normalised library, directionally cloned into pSPORT1. Derived from maternal tissue dissected from developing grains (10 days post anthesis) in glasshouse grown barley plants. Developed as part of the barley transcriptome resources of BBSRC/SEERAD funded cereal IGF (Investigating Gene Function) project.

text: 10 DPA

characteristic: dev-stage

text: Hordeum vulgare subsp. vulgare

characteristic: organism

text: Sal I

text: Not I

text: maternal

text: maternal, 10 DPA, no treatment, cv Optic, EBma04

text: Plasmid


text: IBDM

text: live

text: Generic

text: Ciona intestinalis

text: directional larval cDNA library

characteristic: title


text: Inra

text: live

text: Generic

text: seedling

text: Pinus pinaster

text: root

characteristic: tissue-type

text: Pinus pinaster root seedling


text: Lilly Research Laboratories

text: live

text: Generic

text: Homo sapiens

text: adipose tissue

text: Homo sapiens adipose tissue


text: Dong-A University

text: live

text: Generic

text: Araneus ventricosus

text: A. ventricosus cDNA Library


text: National Institute for Basic Biology

text: live

text: Generic

text: adult

text: Oryzias latipes

text: HNI

characteristic: strain

text: ovary

text: Medaka ovary cDNA library (OLd)


text: National Institute for Basic Biology

text: live

text: Generic

text: Oryzias latipes

text: HNI

text: UV irradiated OLHNI cell line cDNA library (OLf)


text: Pioneer Hi-Bred International, Inc.

text: live

text: Generic

text: SOLR

text: Zea mays

text: EcoRI

text: XhoI

text: Premeitoic>early uninucleate tassel

text: plasmid

text: ZAP II/PBSK


text: Pioneer Hi-Bred International, Inc.

text: live

text: Generic

text: XLOLR

text: Zea mays

text: EcoRI

text: XhoI

text: Immature ear

text: plasmid

text: pBK-CMV


text: Pioneer Hi-Bred International, Inc.

text: live

text: Generic

text: Zea mays

text: SalI

text: NotI

text: Black Mexican Sweet corn-P2#10

text: plasmid


text: Pioneer Hi-Bred International, Inc.

text: live

text: Generic

text: Zea mays

text: SalI

text: NotI

text: Leaf, mutator transposon containing material

text: plasmid


text: Pioneer Hi-Bred International, Inc.

text: live

text: Generic

text: Zea mays

text: SalI

text: NotI

text: Ear shoot, prophaseI

text: plasmid


text: Pioneer Hi-Bred International, Inc.

text: live

text: Generic

text: Zea mays

text: SalI

text: NotI

text: Heat Shock recovery seedling (8hr)

text: plasmid


text: Pioneer Hi-Bred International, Inc.

text: live

text: Generic

text: Zea mays

text: SalI

text: NotI

text: Mechanically wounded leaves (1 hr)

text: plasmid


text: Pioneer Hi-Bred International, Inc.

text: live

text: Generic

text: Zea mays

text: SalI

text: NotI

text: Green leaves, JA treated, normalized

text: plasmid


text: Pioneer Hi-Bred International, Inc.

text: live

text: Generic

text: Zea mays

text: SalI

text: NotI

text: 30 DAP Embryo

text: plasmid


text: Pioneer Hi-Bred International, Inc.

text: live

text: Generic

text: Zea mays

text: SalI

text: NotI

text: Regenerated callus, 5 days post auxin

text: plasmid


text: Pioneer Hi-Bred International, Inc.

text: live

text: Generic

text: Zea mays

text: SalI

text: NotI

text: Regenerating callus, 10 & 14 days after auxin removal

text: plasmid


text: Pioneer Hi-Bred International, Inc.

text: live

text: Generic

text: Zea mays

text: SalI

text: NotI

text: Corn Root Worm infested roots

text: plasmid


text: Pioneer Hi-Bred International, Inc.

text: live

text: Generic

text: Zea mays

text: SalI

text: NotI

text: V5 Roots

text: plasmid


text: Pioneer Hi-Bred International, Inc.

text: live

text: Generic

text: Zea mays

text: SalI

text: NotI

text: root tips, < 5mm in length

text: plasmid


text: Pioneer Hi-Bred International, Inc.

text: live

text: Generic

text: Zea mays

text: SalI

text: NotI

text: Pooled Heat Shock seedling

text: plasmid


text: Pioneer Hi-Bred International, Inc.

text: live

text: Generic

text: XLOLR

text: Zea mays

text: EcoRI

text: XhoI

text: Cob before pollination

text: plasmid

text: pBK-CMV


text: Pioneer Hi-Bred International, Inc.

text: live

text: Generic

text: Zea mays

text: SalI

text: NotI

text: Green leaves, normalized

text: plasmid


text: Pioneer Hi-Bred International, Inc.

text: live

text: Generic

text: Zea mays

text: SalI

text: NotI

text: Germinating maize seeds, 2 & 3 days, roots

text: plasmid


text: Pioneer Hi-Bred International, Inc.

text: live

text: Generic

text: Zea mays

text: SalI

text: NotI

text: 13 DAP dissected endosperm

text: plasmid


text: Pioneer Hi-Bred International, Inc.

text: live

text: Generic

text: Zea mays

text: SalI

text: NotI

text: 7 DAP whole kernels

text: plasmid


text: Pioneer Hi-Bred International, Inc.

text: live

text: Generic

text: Zea mays

text: SalI

text: NotI

text: V9 7cm whorl section+ECB1, screened 1

text: plasmid


text: Pioneer Hi-Bred International, Inc.

text: live

text: Generic

text: Zea mays

text: SalI

text: NotI

text: Intercalary meristem of expanding internodes 5-9. Sampled @ V10 stage

text: plasmid


text: Pioneer Hi-Bred International, Inc.

text: live

text: Generic

text: Zea mays

text: SalI

text: NotI

text: Night harvested leaf tissue; V8-V10 stage

text: plasmid


text: Pioneer Hi-Bred International, Inc.

text: live

text: Generic

text: Zea mays

text: SalI

text: NotI

text: pooled primary & secondary immature ear

text: plasmid


text: Iowa State University

text: live

text: Generic

text: First-strand cDNA was prepared from oligo-dT selected mRNA by priming with an XhoI oligo-dT primer (5' GAGAGAGAGAGAGAGAGAGAACTAGTCTCGAGTTTTTTTTTTTTTTTTTT 3'). The resulting DNA:RNA hybrid was treated with RNase H and used as a template for DNA PolI-catalyzed second strand synthesis. After the addition of EcoRI adaptors, ds-cDNAs were digested with XhoI. Molecules between 0.5 and 1.5 kb were directionally cloned into the EcoRI and XhoI sites of the lambdaZAP-CMV XR vector. The resulting phage library was converted to a pCMV-Script EX phagemid library by en masse in vivo excision. DNA isolated from the phagemid library was digested with NotI to remove empty vector clones. Linear DNAs from 4.8 to 6 kb were gel purified and ligated at low concentration to promote recircularization. Ligation products were precipitated and transformed into DH10B host cells. The complexity of the resulting library was 6 x 10^6.

characteristic: description

text: Zea mays

text: EcoRI

text: XhoI

text: The above-ground portion of one-month old plants (excluding the blades and sheaths of fully expanded leaves

text: pCMV-Script EX (4.3 kb, Stratagene)


text: Iowa State University

text: live

text: Generic

text: Tissue samples were collected and partially pooled prior to RNA extraction. First-strand cDNAs were prepared from 21 individual pools of oligo-dT selected mRNAs by priming with 21 different NotI oligo-dT tag primers (5'-AACTGGAAGAATTCGCGGCCGCNNNNNNTTTTTTTTTTTTTTTTTT-3'). Distinguishable bar code tags, (N)6, were used for each separate first-strand cDNA synthesis. Hence, these bar code tags can be used to identify the mRNA pool from which a particular cDNA clone was derived. The 'bar code' tags associated with specific tissue sources are: ATACGC--Germinated seeds and seedlings (1, 2, 8, 11 DAG); ACTGGC--Mixed mature tissues (17, 21, 38, 69, 77 DAG); CACAGC--Kernels (3, 5, 10, 15, 20, 25, 30, DAP); TAACCC--Adventious roots (65 DAG); CAGGCG--Tassels (3-39 cm, 53 and 56 DAG); AGGTAC--Immature ears (0.2-3.0 cm, 53, 56, 59 DAG); TGAGCG--Husks (73 DAG); GACCAC--Silks; AATCGG--unpollinated first ears; CTAAGG--ear shanks; GAAGAG--etiolated seedlings; AGTGAG--callus; GTGGAC--Cycloheximide-treated callus; GTCACC--Anaerobic treated seedlings; CGTCCA--NAA (a-Naphthalene acetic acid)-treated seedlings; GATGCC--Kinetin-treated seedlings; AAGACC--ACPC (1-aminocyclopropane-1-carboxylix acid)-treated seedlings; GCCTCA--Brassinolide-treated seedlings; CTAGCC--ABA (Abscisic acid)-treated seedlings; TACGGA--GA (Gibberellic acid)-treated seedlings; GCAGGA--JA (Jasmonic acid)-treated seedlings; Equal amounts of first-strand cDNA from each reaction were combined and used as template for DNA PolI-catalyzed 2nd strand synthesis. After the addition of EcoRI adaptors, ds-cDNAs were digested with NotI. Molecules between 0.5 and 2.0 kb were directionally cloned into the EcoRI and NotI sites of the pSlip7 expression vector. Plasmid DNA isolated from the library was digested with NotI to remove empty vector clones. Linear DNAs from 5.4 to 7 kb were gel purified and ligated at low concentration to promote recircularization. Ligation products were precipitated and transformed into DH10B host cells. The complexity of the resulting library was 1.2 x 10X6.

text: Zea mays

text: EcoRI

text: NotI

text: mixed

text: pSlip7 (4.43 kb)

characteristic: vector


text: UCLA School of Medicine

characteristic: INSDC last update

text: live

text: Generic

text: genes commonly expressed in peripheral tissues were subtracted from blood-brain barrier cDNA with the suppression subtractive hybridization (SSH) method using driver cDNA produced from a pool of rat liver and kidney derived poly A+ RNA.

text: adult

text: XL1 blue

text: Rattus norvegicus

text: male

characteristic: sex

text: Sprague-Dawley

text: blood-brain barrier

text: Rat blood-brain barrier specific cDNA library

text: plasmid

text: pCRII (Invitrogen)


characteristic: External Id

text: Children's Memorial Research Center

characteristic: INSDC center name

text: live

text: Generic

text: The UI-R-CN1 library is a subtracted library derived from the following pool of seven normalized rat libraries: normalized rat seminal vesicles, normalized rat penis, normalized rat bladder, normalized rat cervix, normalized rat brown adipose, normalized rat fundus, and normalized rat salivary gland. It was constructed according to the procedure described by Bonaldo, Lennon & Soares (Genome Research Genome 6: 791-806, 1996). For construction of the CN1 library, plasmid DNA from the pool of normalized libraries was electroporated into competent bacteria for the production of single-stranded circular DNA. This was then used as a tracer in a subtractive hybridization with a driver (PCR amplified inserts from a plamid DNA template preparation) comprising: a) a pool of about 34,000 clones from the Rat Unigene Set corresponding to plates R-5-AA-NN excluding plates R-5-MM and MN. This pool represented 40% of the final driver population. b) a pool of about 29,000 clones from subtracted libraries CA0 and CA1 corresponding to plates R-CA0-AWV through R-CA0-AXS, R-CA0-AZX through R-CA0-BAZ, R-CA0-BFE through R-CA0-BHY, R-CA0-BJS, R-CA0-BKE, R-CA0-BKG-H, R-CA0-BKJ-K, R-CA0-BKP through R-CA0-BKS, R-CA0-BKU-V, R-CA0-BLY through R-CA0-BMA, R-CA0-BMC through R-CA0-BME, R-CA0-BNS, R-CA0-BOB through R-CA0-BOJ, R-CA0-BPA through R-CA0-BPG, R-CA1-BBA through R-CA1-BDA, R-CA1-BHZ through R-CA1-BJF, R-CA1-BJR, R-CA1-BJT through R-CA1-BKB, R-CA1-BKD, R-CA1-BKF, R-CA1-BKI, R-CA1-BKT, R-CA1-BLF, R-CA1-BLH through R-CA1-BLN, R-CA1-BLS, R-CA1-BLU-V, R-CA1-BNR, and R-CA1-BLE. The resulting pool represented 20% of the final driver population. c) a pool of about 15,000 clones from non-normalized libraries CS0s, CT0s, CU0s, CW0s, CX0s and normalized libaries CS0, CT0, CU0, CW0, and CX0 corresponding to plates R-CS0s-CBD through R-CS0s-CBO, R-CT0s-CAM through R-CT0s-CAX, R-CU0s-CBP through R-CU0s-CCA, R-CW0s-CCB through R-CW0s-CCM, R-CX0s-CCN through R-CX0s-CCX, R-CS0-BSD, R-CS0-BTD through R-CS0-BTV, R-CS0-BVM, R-CT0-BTW through R-CT0-BUP, R-CT0-BVN, R-CU0-BUQ through R-CU0-BVL, R-CW0-BVV through R-CW0-BWP, R-CW0-BXN through R-CW0-BXO, R-CX0-BWQ through R-CX0-BXM. The resulting pool represented 5% of the final driver population. d) a pool of about 5,000 clones (1,000 from non-normalized eye library CV0 and 4,000 from normalized eye library CV1) corresponding to plates R-CV0-BRH through R-CV0-BRR, R-CV1-BRS through R-CV1-BSC, R-CV1-BSE through R-CV1-BTC, and R-CV1-BVO through R-CV1-BVU. This pool represented about 5% of the final driver population. e) A pool of about 10,000 clones from subtracted library BS2, BV0 and BV0p (7-9.5 kb cDNA library fraction from rat whole embryo), and BX0 (0.5-7kb cDNA library fraction from rat whole embryo) corresponding to plates R-BS2-BDB through R-BS2-BFB, R-BV0-ANK through R-BV0-ANR, R-BV0p-AOI through R-BV0p-AOX, and R-BX0-AQY through R-BX0-ASH. The resulting pool represented 5% of the final driver population. f) a pool of about 7,000 clones from the seven non-normalized libraries that make up the tracer including CY0, CZ0, DA0, DB0, DC0, DD0, and DE0 corresponding to plates R-CY0-BXP through R-CY0-BXZ, R-CZ0-BYA through R-CZ0-BYI, R-CZ0-BZB-C, R-DA0-BYJ through R-DA0-BYP, R-DA0-BZD through R-DA0-BZH, R-DB0-BYQ through R-DB0-BZA, R-DC0-BZI through R-DC0-BZQ, R-DC0-CAY through R-DC0-CBA, R-DD0-BZR through R-DD0-CAA, R-DD0-CBB-C, and R-DE0-CAB through R-DE0-CAL. The resulting pool represented about 10% of the final driver population. g) a pool of about 2,000 clones from the pool of normalized libraries, CN0, that makes up the tracer. The corresponding plates are R-CN0-BKW through R-CN0-BLD, R-CN0-BLG, R-CN0-BLP through R-CNO-BLR, R-CN0-BLT, R-CN0-BLW-X, R-CN0-BMB, and R-CN0-BMF through R-CN0-BML. This pool represented 5% of the final driver population. h) a pool of the 28 most abundant clones in the CN0 pool corresponding to the following addresses: bkw-a-09-0-UI, bkw-b-09-0-UI, bkw-b-11-0-UI, bkx-b-10-0-UI, bkx-d-01-0-UI, bkx-d-06-0-UI, bkx-g-08-0-UI, bkx-h-12-0-UI, bky-a-05-0-UI, bkz-a-06-0-UI, bkz-a-11-0-UI, bkz-c-06-0-UI, bkz-c-09-0-UI, bkz-d-10-0-UI, bla-a-01-0-UI, bla-a-02-0-UI, bla-f-04-0-UI, bla-g-07-0-UI, bla-g-12-0-UI, blb-a-12-0-UI, blb-f-02-0-UI, blc-a-11-0-UI, blc-e-95-0-UI, bld-1-08-0-UI, bld-f-02-0-UI, blq-h-04-0-UI, blr-a-05-0-UI, blt-f-08-0-UI. This pool represented 5% of the final driver population. i) One abundant CN0 clone (corresponding to the address bkz-a-11-0-UI) was digested with Not I and Eco RI and the resulting insert was gel purified. This purified insert was added directly to the driver so that it represented 5% of the final driver population.

text: adult

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid


text: Children's Memorial Research Center

characteristic: INSDC first public

text: live

text: Generic

text: The UI-R-DM1 library is a normalized Rat Prostate library (nRPro) constructed in pT3T7 PAC vector according to the procedure described by Bonaldo, Lennon & Soares (Normalization and Subtraction: Two Approaches to Facilitate Gene Discovery. Genome Research 6: 791-806, 1996) The oligonucleotide used to prime first strand synthesis contained the sequence tag CCAGG between the Not I cloning site and dT18 stretch. The Rat Prostate tissue was provided by Tom Freeman of the Sanger Center.

text: adult

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid


text: Children's Memorial Research Center

text: live

characteristic: INSDC status

text: Generic

text: The UI-R-DN1 library is a normalized Rat Distal Colon library (nRDC) constructed in pT3T7 PAC vector according to the procedure described by Bonaldo, Lennon & Soares (Normalization and Subtraction: Two Approaches to Facilitate Gene Discovery. Genome Research 6: 791-806, 1996). The oligonucleotide used to prime first strand synthesis contained the sequence tag GAAGTGCTCC between the Not I cloning site and DT18 stretch. The Rat Distal Colon tissue was provided by Tom Freeman of the Sanger Center.

text: adult

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid


text: Children's Memorial Research Center

text: live

text: Generic

text: The UI-R-DO1 library is a normalized Rat Cell Line R3327-5P library (nR5P) constructed in pT3T7 PAC vector according to the procedure described by Bonaldo, Lennon & Soares (Normalization and Subtraction: Two Approaches to Facilitate Gene Discovery. Genome Research 6: 791-806, 1996.) The oligonucleotide used to prime first strand synthesis contained the sequence tag CACGTGAGAT between the Not I cloning site and dT18 stretch. The rat cell line R3327-5P was provided by Mary Hendrix of the University of Iowa.

text: adult

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid


text: National Cancer Institute / NIH

text: live

text: Generic

text: UI-R-DY0 is a non-normalized Rat cartilage library (RC) constructed in pT3T7 PAC vector according to the procedure described by Bonaldo, Lennon & Soares (Genome Research Genome 6: 791-806, 1996). The oligonucleotide used to prime first strand synthesis contained the sequence tag CTAATGGACG between the Not I cloning site and dT18 stretch. The Rat cartilage tissue was provided by Dr Jeff Stevens at the University of Iowa.

text: adult

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid


text: Children's Memorial Research Center

text: live

text: Generic

text: The UI-R-DR1 library is a normalized Rat Osteoblast library (nREO) constructed in pT3T7 vector according to the procedure described by Bonaldo, Lennon & Soares (Normalization and Subtraction: Two Approaches to Facilitate Gene Discovery. Genome Research 6: 791-806, 1996). The oligonucleotide used to prime first strand synthesis contained the sequence tag AAGATATCAA between the Not I cloning site and dT18 stretch. The Rat Osteoblast tissue was provided by Lian & Stein of the University of Massachussetts Medical School.

text: adult

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid


text: Children's Memorial Research Center

text: live

text: Generic

text: UI-R-EA0 is a non-normalized Rat Ileum library (RIL) constructed in pT3T7 PAC vector according to the procedure described by Bonaldo, Lennon & Soares (Genome Research Genome 6: 791-806, 1996). The oligonucleotide used to prime first strand synthesis contained the sequence tag CGACACCTCG between the Not I cloning site and dT18 stretch. The Rat Ileum tissue was provided by Tom Freeman of the Sanger Center.

text: adult

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid


text: Children's Memorial Research Center

text: live

text: Generic

text: UI-R-EB1 is a non-normalized Rat Duodenum library (RDU) constructed in pT3T7 PAC vector according to the procedure described by Bonaldo, Lennon & Soares (Genome Research Genome 6: 791-806, 1996). The oligonucleotide used to prime first strand synthesis contained the sequence tag TGTGGTTCAT between the Not I cloning site and dT18 stretch. The Rat Deuodenum tissue was provided by Tom Freeman of the Sanger Center.

text: adult

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid


text: Children's Memorial Research Center

text: live

text: Generic

text: UI-R-EB0 is a non-normalized Rat Duodenum library (RDU) constructed in pT3T7 PAC vector according to the procedure described by Bonaldo, Lennon & Soares (Genome Research Genome 6: 791-806, 1996). The oligonucleotide used to prime first strand synthesis contained the sequence tag TGTGGTTCAT between the Not I cloning site and dT18 stretch. The Rat Deuodenum tissue was provided by Tom Freeman of the Sanger Center.

text: adult

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid


text: National Cancer Institute / NIH

text: live

text: Generic

text: UI-R-DZ0 is a non-normalized Rat cartilaginous tumor library (RCT) constructed in pT3T7 PAC vector according to the procedure described by Bonaldo, Lennon & Soares (Genome Research Genome 6: 791-806, 1996). The oligonucleotide used to prime first strand synthesis contained the sequence tag CATTCTTGTA between the Not I cloning site and dT18 stretch. The Rat cartilaginous tumor tissue was provided by Dr Jeff Stevens at the University of Iowa.

text: adult

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid


text: Children's Memorial Research Center

text: live

text: Generic

text: The UI-R-DQ1 library is a normalized Rat Cell Line R3327-5A library (nR5A) constructed in pT3T7 PAC vector according to the procedure described by Bonaldo, Lennon & Soares (Normalization and Subtraction: Two Approaches to Facilitate Gene Discovery. Genome Research 6: 791-806, 1996). The oligonucleotide used to prime first strand synthesis contained the sequence tag GGACTAGATC between the Not I cloning site and dT18 stretch. The Rat Cell Line R3327-5A was provided by Mary Hendrix of the University of Iowa.

text: adult

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid


text: Institute for Systems Biology

text: live

text: Generic

text: Instruction manual 'Superscript plasmid systems for cDNA synthesis and plasmid cloning' (Life Technologies ) was followed. In brief, cDNAs were synthesized with a Not-I primer adapter. A Sal-I adapter is then ligated to the cDNAs. The resulting cDNAs were digested with NotI, size fractionated and cloned into NotI-SalI digested pSportI plasmid vector.

text: Prostate

characteristic: organ

text: Homo sapiens

text: NotI

text: SalI

text: Male

text: plasmid

text: pSportI


text: University of Chicago

text: live

text: Generic

text: Details on library preparation can be found in Chilcoat and Turkewitz (2001) Proc. Natl. Acad. Sci USA, 98: 8709-8713.

text: Tetrahymena thermophila

text: Chilcoat/Turkewitz cDNA (small fraction)

text: BlueScript2 SK+


text: University of Chicago

text: live

text: Generic

text: Tetrahymena thermophila

text: Chilcoat/Turkewitz cDNA (large fraction)

text: BlueScript2 SK+


text: National Eye Institute of China, Zhongshan Ophthalmic Center, Zhongshen(Sun Yat-Sen) University

text: live

text: Generic

text: The sequences were from the retina of retinal degeneration slow (rds) mouse during the development of retinitis pigmentosa by the differential display experiments. The rds mouse and the normal control C3B mouse were purchased from the Jackson Laboratory (600 Main St., Bar Harbor, Maine 04609, USA). Most of the sequences were identified by Northern blotting. In the publication (PMID: 11295129), we only reported five sequences. We submit all of them now.

characteristic: lab-host

text: Mus musculus

text: HindIII

text: HindIII

text: male and female

text: inbred congenic rds mouse (JR1999 C3H-Prph2Rd2)

text: Retina

text: cDNA fragments (3'end of differential display band) of the rds mouse

text: plasmid

text: pBluescript and pGEM-TEasy(Promega)


text: National Institute of Genetics

text: live

text: Generic

characteristic: NCBI submission model

text: Akashinriki

characteristic: cultivar

text: vegetative stage

text: Hordeum vulgare subsp. vulgare

text: leaves

text: K. Sato unpublished cDNA library, cv. Akashinriki vegetative stage leaves


text: National Institute of Genetics

text: live

text: Generic

characteristic: NCBI submission package

text: Haruna Nijo

text: germination

characteristic: dev-stage

text: Hordeum vulgare subsp. vulgare

text: shoots

text: K. Sato unpublished cDNA library, cv. Haruna Nijo germination shoots


text: National Institute of Genetics

text: live

text: Generic

text: Haruna Nijo

text: second leaf stage

text: Hordeum vulgare subsp. vulgare

characteristic: organism

text: seedling leaves

text: K. Sato unpublished cDNA library, cv. Haruna Nijo second leaf stage seedling leaves

characteristic: title


text: Melaka Institute of Biotechnology

text: live

text: Generic

text: Somatic

characteristic: cell type

text: oleifera

text: We followed all the instructions as per the the protocol given by manufacturer (Stratagene) of the cDNA synthesis kit.

text: 15 week after anthesis (WAA)

text: Fruit

text: Elaeis oleifera

text: EcoRI

text: XhoI

text: Mesocarp

characteristic: tissue-type

text: Oil palm Lambda Zap-II Express Library

text: Lambda Zap-II


text: National Institute of Genetics

text: live

text: Generic

text: Haruna Nijo

text: adult, heading stage

text: Hordeum vulgare subsp. vulgare

text: top three leaves

text: K. Sato unpublished cDNA library, cv. Haruna Nijo adult, heading stage top three leaves


text: National Institute of Genetics

text: live

text: Generic

text: adult, heading stage

text: Hordeum vulgare subsp. spontaneum

characteristic: strain

text: top three leaves

text: K. Sato unpublished cDNA library, strain H602 adult, heading stage top three leaves


text: National Institute of Genetics

characteristic: INSDC last update

text: live

text: Generic

text: Caenorhabditis elegans

text: hermaphrodite

text: whole animal

text: unpublished oligo-capped cDNA library, C. elegans L1 stage


text: University of Nevada

text: live

text: Generic

text: Library construction was performed according to manufacture's (Stratagene, Inc.) recommended protocol for the Lambda UniZapXR vector and cDNA synthesis kit.

characteristic: description

text: adult

text: Selaginella lepidophylla

text: EcoRI

text: XhoI

text: microphyll fronds undergoing desiccation for 2.5 h

text: An expressed sequence tag (EST) collection from the resurrection plant Selaginella lepidophylla

text: Phagemid

text: Lambda Uni-Zap XR, Bluescript SK-

characteristic: vector


characteristic: External Id

text: Henan Normal Univ

characteristic: INSDC center name

text: live

text: Generic

text: Rattus norvegicus

text: male

characteristic: sex

text: Sprague-Dawley

text: liver

text: Rat male liver


text: The Third Hospital of Peking University

characteristic: INSDC first public

text: live

characteristic: INSDC status

text: Generic

text: Homo sapiens

text: invasive hydatidiform mole

text: Homo sapiens invasive hydatidiform mole


text: University of Groningen

text: live

text: Generic

characteristic: NCBI submission model

text: Homo sapiens

text: Expressed sequences from YACs


text: The University of Hong Kong

text: live

text: Generic

text: Trichinella spiralis

text: Trichinella spiralis library


text: Texas A&M University

text: live

text: Generic

text: cDNA library constructed by Clontech and donated by Nancy Ing, Dept. of Animal Science, Texas A&M University. DNA sequencing by DNA Core Facility, The Noble Foundation, Ardmore, Oklahoma. Clone distribution by L.C. Skow. Send e-mail to: lskow@cvm.tamu.edu.

text: XL1-Blue MRF Genotype

characteristic: lab-host

text: Testis

characteristic: organ

text: Equus caballus

text: EcoR I

text: Male

text: American Quarter Horse

text: Ing horse testis cDNA

text: Plasmid

text: Lambda ZAP II Custom cDNA Library


text: Pioneer Hi-Bred International, Inc.

text: live

text: Generic

characteristic: NCBI submission package

text: Zea mays

text: SalI

text: NotI

text: Tassel: Apical meristem > floral transition; screened 1

text: plasmid


text: Jilin Academy of Agricultural Sciences

text: live

text: Generic

text: The cDNA fragment was obtained from soybean by differential display and the northern blotting showed that it is real positive signal.

text: seedlings

text: Glycine max

characteristic: organism

text: Glycine max seedlings differential display


text: Yonsei University College of Medicine

text: live

text: Generic

text: Clonorchis sinensis

text: EcoRI

text: XhoI

text: Clonorchis sinensis cDNA Library

characteristic: title

text: pBK-CMV


text: National Institute of Genetics

text: live

text: Generic

text: synonym: Physcomitrella patens subsp. patens

text: Physcomitrium patens

text: mixture of chloronemata and young gametophores with 2 to 5 leaves

text: full length cDNA library, chloronemata and young gametophores


text: National Institute of Genetics

text: live

text: Generic

text: Physcomitrium patens

text: mixture of chloronemata, caulonemata and malformed buds

text: normalized full length cDNA library, chloronemata, caulonemata and malformed buds


text: National Institute of Genetics

text: live

text: Generic

text: Physcomitrium patens

text: mixture of chloronemata, caulonemata and rhizoid-like protonemata

text: normalized full length cDNA library, chloronemata, caulonemata and rhizoid-like protonemata


text: Juntendo University School of Medicine

text: live

text: Generic

text: Oryctolagus cuniculus

text: corneal endothelium

text: Oryctolagus cuniculus corneal endothelium


text: Southern Illinois University at Carbondale

text: live

text: Generic

text: Obese female Zucker rats were fed with different levels of soy-isoflavones as dietary supplements. Total RNA was isolated from the liver of control, low isoflavone and high isoflavone treatment and differentially expressed ESTs were identified by differential display of mRNA. The ESTs were cloned in pGEM vector. ESTs were sequenced using M13 universal forward or reverse primer.

text: Liver

text: Rattus norvegicus

text: Female

text: Zucker (fa/fa)

text: Differentially expressed ESTs in rat liver

text: Plasmid

text: pGEM


text: Stanford University

text: live

text: Generic

text: BMS (Black Mexican Sweet)

characteristic: cultivar

text: The library was prepared by George Rudenko using poly (A) selected RNA and Universal Riboclone cDNA Synthesis System (Promega). cDNA was synthesized using both random and oligo(dT) primers in separate reactions and equipped with EcoRI adaptors. Library was size-fractionated on agarose gels (for insert size >400bp) and non-directionally cloned into EcoRI-digested pUC19 vector. Blue/white selection on carbenicillin-containing plates was used to recover positive clones.

text: mixed logarithmic and stationary growth phases

characteristic: dev-stage

text: Zea mays

text: EcoRI

text: EcoRI

text: suspension culture

text: 952 - BMS tissue from Walbot Lab (reduced rRNA)

text: plasmid


text: Institute of Basic Medical Sciences; Chinese Academy of Medical Sciences & Peking Union Medical College; Chinese National Human Genome Center, Beijing

text: live

text: Generic

text: The cells of Primary Spermatocyte and Round Spermatid were isolated from Human testis. Total RNA was extracted by TRIzol. Using PCR-Select cDNA Subtraction Kit, the library was constructed

text: adult

text: Homo sapiens

text: male

text: testis

characteristic: tissue-type

text: Human testis cDNA library


characteristic: INSDC last update

text: live

text: Generic

text: The cells of Primary Spermatocyte and Round Spermatid were isolated from Rat testis. Total RNA was extracted by TRIzol. Using PCR-Select cDNA Subtraction Kit, the library was constructed

text: adult

text: Rattus norvegicus

text: male

text: testis

text: Rat testis cDNA library


text: IACR-Rothamsted

text: live

text: Generic

text: Darius

text: RNA was prepared from dark-grown plants treated with cloquintocet-mexyl and phenobarbital.

characteristic: description

text: 7 day old

text: Triticum aestivum

text: shoot

text: safener-induced wheat cDNA fragments

text: plasmid

text: pCR 2.1 (Invitrogen)


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: RNA source male hippocampus, age 27. Library is oligo-dT primed and directionally cloned (EcoRV site is destroyed upon cloning). Average insert size 1.4 kb, insert size range 0.9-4 kb. Library is normalized and enriched for full-length clones and was constructed by C. Gruber (Invitrogen). Research Genetics tracking code 012.

text: brain

text: Homo sapiens

text: EcoRV (destroyed)

text: NotI

text: hippocampus

text: plasmid (ampicillin resistant)

characteristic: vector


characteristic: External Id

text: University of Washington

text: live

text: Generic

text: adult

text: Macaca mulatta

text: spleen

text: Rhesus


text: Auburn University

characteristic: INSDC center name

text: live

text: Generic

text: Spleen

text: Ictalurus punctatus

text: NotI

text: SalI

text: Spleen cDNA library

text: Phagemid


text: University of Delaware

text: live

text: Generic

text: Library made from equivalent pools of total RNA isolated from each developmental age (across strains); Single pass sequencing from 5'-end

text: Embryonic (d18,d19); post-hatch (d1,w3,w7,w9,w16,1yr)

text: E. coli EMDH10B

text: Gallus gallus

text: Male and Female

characteristic: sex

text: Commercial broiler, Ottawa Research Centre, leghorn

characteristic: strain

text: Abdominal Fat

text: Primary Chicken Abdominal Fat Library (pgf2c)


text: University of Delaware

characteristic: INSDC first public

text: live

characteristic: INSDC status

text: Generic

text: E. coli EMDH10B

text: Gallus gallus

text: Male and Female

text: Abdominal Fat

text: Normalized Chicken Abdominal Fat Library (pgf2n)


text: North Carolina State University

text: live

text: Generic

characteristic: NCBI submission model

text: Root (primary)

characteristic: cell type

text: The library is from primary xylem scraped from the roots of a twelve year old tree in the transitional phase from juvenile wood to mature wood production. NOTE: The sequences contain a 'cDNA adapter' between the EcoRI site and the start of the EST. The adaptersequence is 'AATTCGGCACGAG'.

text: Transitional

characteristic: lab-host

text: Pinus taeda

text: Eco RI

text: XhoI

text: Coastal plain loblolly pine from North Carolina

text: Xylem

text: NXRV (Nsf Xylem Root wood Vertical)

text: pBlueScript SK-


text: Dept of AFNS, University of Alberta

text: live

text: Generic

characteristic: NCBI submission package

text: Stratified squamous epithelial

text: Young adult

text: Omasum

characteristic: organ

text: Bos taurus

characteristic: organism

text: EcoRI

text: Xho I

text: Smooth muscle

text: Bos taurus Omasum #1 library


text: Dept of AFNS, University of Alberta

text: live

text: Generic

text: Stratified squamous epithelial

text: Young adult

characteristic: dev-stage

text: Reticulum

text: Bos taurus

text: EcoRI

text: Xho I

text: Smooth muscle

text: Bos taurus Reticulum #1 library

characteristic: title


text: Dept of AFNS, University of Alberta

characteristic: INSDC last update

text: live

text: Generic

text: Stratified squamous epithelial

text: Young adult

text: Rumen

text: Bos taurus

text: EcoRI

text: Xho I

text: Smooth muscle

characteristic: tissue-type

text: Bos taurus Rumen #1 library


text: University of Nevada

text: live

text: Generic

text: Library construction was performed according to Stratagene's recommended protocol for the Lambda UniZapXR vector and cDNA synthesis kit.

characteristic: description

text: juvenile and adult

text: Vitis vinifera

text: EcoRI

text: XhoI

text: leaf

text: An expressed sequence tag database for abiotic stressed leaves of Vitis vinifera var. Chardonnay

text: Phagemid

text: Lambda Uni-Zap XR, Bluescript SK-

characteristic: vector


characteristic: External Id

text: Auburn University

characteristic: INSDC center name

text: live

text: Generic

text: Liver

text: Ictalurus punctatus

text: NotI

text: SalI

text: Liver cDNA library

text: Phagemid


text: University of Delaware

text: live

text: Generic

text: Library made from three total RNA pools from each tissue (testis 25%, ovary 25%, and oviduct 50% of final RNA pool); Single pass sequencing from 5'-end

text: Various stages;embryonic, post-hatch, immature and sexually-mature

text: E. coli EMDH10B

text: Gallus gallus

text: Male and Female

characteristic: sex

text: Commercial broiler and layer

characteristic: strain

text: Testis, ovary and oviduct

text: Primary Chicken Reproductive Tract cDNA Library (pgr1c)


text: University of Delaware

characteristic: INSDC first public

text: live

characteristic: INSDC status

text: Generic

text: E. coli EMDH10B

text: Gallus gallus

text: Male and Female

text: Commercial broiler and layer

text: Testis, ovary and oviduct

text: Normalized Chicken Reproductive Tract cDNA Library (pgr1n)


text: Scottish Crop Research Institute

text: live

text: Generic

characteristic: NCBI submission model

text: Optic

characteristic: cultivar

text: Non-normalised library, directionally cloned into pSPORT1. Derived from carpels dissected from developing flowers in glasshouse grown barley plants. Developed as part of the barley transcriptome resources of BBSRC/SEERAD funded cereal IGF (Investigating Gene Function) project.

text: pre-anthesis

text: Hordeum vulgare subsp. vulgare

text: Sal I

text: Not I

text: carpel

text: carpel, pre-anthesis, no treatment, cv Optic, EBca01

text: Plasmid


text: Scottish Crop Research Institute

text: live

text: Generic

characteristic: NCBI submission package

text: Optic

text: Non-normalised library, directionally cloned into pSPORT1. Derived from endosperm tissue dissected from developing grains (8 days post anthesis) in glasshouse grown barley plants. Developed as part of the barley transcriptome resources of BBSRC/SEERAD funded cereal IGF (Investigating Gene Function) project.

text: 8 DPA

characteristic: lab-host

text: Hordeum vulgare subsp. vulgare

text: Sal I

text: Not I

text: endosperm

text: endosperm, 8 DPA, no treatment, cv Optic, EBed02

text: Plasmid


text: Scottish Crop Research Institute

text: live

text: Generic

text: Optic

text: Non-normalised library, directionally cloned into pSPORT1. Derived from embryos dissected from germinating grains (2 day) in glasshouse grown barley plants. Developed as part of the barley transcriptome resources of BBSRC/SEERAD funded cereal IGF (Investigating Gene Function) project.

text: 2 Day germination

text: Hordeum vulgare subsp. vulgare

characteristic: organism

text: Sal I

text: Not I

text: embryo

text: embryo, 2 Day germination, no treatment, cv Optic, EBem10

text: Plasmid


text: Scottish Crop Research Institute

text: live

text: Generic

text: Optic

text: Non-normalised library, directionally cloned into pSPORT1. Derived from endosperm tissue dissected from developing grains (28 days post anthesis) in glasshouse grown barley plants. Developed as part of the barley transcriptome resources of BBSRC/SEERAD funded cereal IGF (Investigating Gene Function) project.

text: 28 DPA

characteristic: dev-stage

text: Hordeum vulgare subsp. vulgare

text: Sal I

text: Not I

text: endosperm

text: endosperm, 28 DPA, no treatment, cv Optic, EBed07

text: Plasmid


text: Scottish Crop Research Institute

text: live

text: Generic

text: Optic

text: Non-normalised library, directionally cloned into pSPORT1. Derived from anthers dissected from developing flowers in glasshouse grown barley plants. Developed as part of the barley transcriptome resources of BBSRC/SEERAD funded cereal IGF (Investigating Gene Function) project.

text: yellow stage

text: Hordeum vulgare subsp. vulgare

text: Sal I

text: Not I

text: anther

text: anther, yellow stage, no treatment, cv Optic, EBan01

characteristic: title

text: Plasmid


text: Scottish Crop Research Institute

characteristic: INSDC last update

text: live

text: Generic

text: Optic

text: Non-normalised library, directionally cloned into pSPORT1. Derived from roots of 3 week old Nitrogen stressed barley plants. Developed as part of the barley transcriptome resources of BBSRC/SEERAD funded cereal IGF (Investigating Gene Function) project.

text: 3 week

text: Hordeum vulgare subsp. vulgare

text: Sal I

text: Not I

text: root

text: root, 3 week, hydroponic grown, low nitrogen, cv Optic, EBro02

text: Plasmid


text: Scottish Crop Research Institute

text: live

text: Generic

text: Optic

text: Non-normalised library, directionally cloned into pSPORT1. Derived from embryos dissected from germinating grains (1 day) in glasshouse grown barley plants. Developed as part of the barley transcriptome resources of BBSRC/SEERAD funded cereal IGF (Investigating Gene Function) project.

text: 1 Day germination

text: Hordeum vulgare subsp. vulgare

text: Sal I

text: Not I

text: embryo

characteristic: tissue-type

text: embryo, 1 Day germination, no treatment, cv Optic, EBem09

text: Plasmid


text: Hema-Quebec

text: live

text: Generic

text: Hybridoma

characteristic: cell type

characteristic: cell-line

text: RACE product from IL-6-starved cells using oligo GAGGTACTCTTCGAGGAGTCCATG

characteristic: description

text: Mus musculus

text: Mus musculus mRNA expressed in hybridoma cells

text: Plasmid

text: pGEM-T


characteristic: External Id

text: University of Nevada

characteristic: INSDC center name

text: live

text: Generic

text: Green

text: Library construction was performed according to Stratagene's recommended protocol for the Lambda UniZapXR vector and cDNA synthesis kit.

text: Dunaliella salina

text: EcoRI

text: XhoI

text: Cells, which was adapted in 2.5M NaCI via a incremental series from 1.7 to 2.0 to 2.25 to 2.5 M NaCl, were exposed to 3.4 M NaCl for 5 hours

text: An expressed sequence tag database for the halotolerant green alga, Dunaliella salina

text: Phagemid

text: Lambda Uni-Zap XR, Bluescript SK-

characteristic: vector


text: University of Delaware

characteristic: INSDC first public

text: live

text: Generic

text: Library made from equivalent pools of total RNA isolated from each tissue (embryonic muscle 33.3%; juvenile muscle 33.3%; and epiphyseal growth plate 33.3% of the final RNA pool). Single pass sequencing from 5'-end

text: Breast,leg:Embryo(d19);post-hatch(1d,1,3,5,7,9,11 weeks);growth plate(1d,7d,14d post-hatch)

text: E. coli EMDH10B

text: Gallus gallus

text: Male and Female

characteristic: sex

text: Commercial broiler and Ottawa Res. Centre Strains 90 & 21

characteristic: strain

text: Breast muscle, leg muscle and epiphyseal growth plate

text: Normalized Chicken Breast Muscle, Leg Muscle, and Epiphyseal Growth Plate cDNA library (pgm1n)


text: University of Delaware

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: E. coli EMDH10B

text: Gallus gallus

text: Male and Female

text: Primary Chicken Breast Muscle, Leg Muscle, and Epiphyseal Growth Plate cDNA library (pgm1c)


text: University of Delaware

text: live

text: Generic

characteristic: NCBI submission package

text: E. coli EMDH10B

characteristic: lab-host

text: Gallus gallus

text: Male and Female

text: Normalized Chicken Breast Muscle, Leg Muscle, and Epiphyseal Growth Plate cDNA library (pgm2n)


text: University of Delaware

text: live

text: Generic

text: Library made from equivalent pools of total RNA isolated from each tissue at different ages. Single pass sequencing from 5'-end

text: Embryonic (d12,d14,d19); post-hatch (1,3,5,7,9 weeks)

characteristic: dev-stage

text: E. coli EMDH10B

text: Gallus gallus

characteristic: organism

text: Male and Female

text: Commercial broiler chickens

text: Pituitary Gland/Hypothalamus/Pineal Gland

text: Normalized Chicken Pituitary/Hypothalamus/Pineal Library (pgp2n)


text: Universitaet zu Koeln

characteristic: INSDC last update

text: live

text: Generic

text: Scherffelia dubia

text: SAG 40.89

text: Scherffelia dubia LambdaZAPII

characteristic: title


text: University of Alabama

text: live

text: Generic

text: Laccaria bicolor in an interaction medium was exposed to Pinus resinosa roots for 6, 12, 24, 36, 48, 72 and 96 hours; the fungal tissue was then collected and frozen. Total mRNA was isolated from Laccaria tissue and pooled. This pooled mRNA was used to prepare a cDNA library. ESTs were randomly selected and sequenced to identify differentially expressed genes induced by root exudates using macroarray analysis.

text: Laccaria bicolor

text: Root-induced cDNA library from Laccaria bicolor


text: Henna Normal Univ.

text: live

text: Generic

text: embryonic day 17 post-fertilization

text: liver

characteristic: organ

text: Rattus norvegicus

text: male

text: Sprague-Dawley

text: Rat liver embryonic day 17 post-fertilization


text: USDA-Agricultural Research Service

text: live

text: Generic

text: Primary library

text: adult

text: brain

text: Ictalurus punctatus

text: Sal 1

text: Not 1

text: female and male

text: Ictalurus punctatus Brain1 primary library

text: plasmid


text: USDA-Agricultural Research Service

text: live

text: Generic

text: Library enriched for clones containing microsatellites.

text: adult

text: brain

text: Ictalurus punctatus

text: Sal 1

text: Not 1

text: female and male

text: Ictalurus punctatus Brain1 microsatellite enriched

text: plasmid


text: Research Institute of Field Surgery, Daping Hospital, Third Military Medical University

text: live

text: Generic

text: Homo sapiens

text: skin

characteristic: tissue-type

text: Human cutaneous subtractive library


text: Mie University, School of Medicine

text: live

text: Generic

text: tadpole

text: Xenopus laevis

text: Xenopus laevis tadpole


text: Friedrich-Alexander-Universitaet Erlangen-Nuernberg

text: live

text: Generic

text: CD4+ T lymphocytes

characteristic: cell type

text: ATL-derived JuanaW

characteristic: cell-line

text: country= Peru

characteristic: description

text: Homo sapiens

text: Homo sapiens ATL-derived JuanaW


characteristic: External Id

characteristic: INSDC center name

text: live

text: Generic

text: CD4+ T lymphocyte

text: HTLV-1-transformed C91-PL

text: Homo sapiens

text: Homo sapiens HTLV-1-transformed C91-PL


text: University of Minnesota

characteristic: INSDC first public

text: live

text: Generic

text: primary hepatocyte

text: Library constructed using PCR-Select cDNA subtraction kit (Clontech, Palo Alto, CA) from various stages of serum-free suspension culture of primary porcine hepatocytes.

text: adult

text: Sus scrofa

text: male

characteristic: sex

text: liver

text: Subtracted porcine hepatocyte cDNA library


text: Pioneer Hi-Bred International, Inc.

text: live

characteristic: INSDC status

text: Generic

text: Zea mays

text: Pioneer AF-1 array


text: Institute of Development, Aging and Cancer, Tohoku University

text: live

text: Generic

characteristic: NCBI submission model

text: hemocytes

text: adult

text: Ciona intestinalis

text: EcoRI

text: Ascidian hemocytes cDNA library

text: Plasmid

characteristic: vector


text: Reproductive Biology Associates

text: live

text: Generic

characteristic: NCBI submission package

text: blastocyst

text: Homo sapiens

text: Homo sapiens blastocyst


text: Washington University School of Medicine

text: live

text: Generic

text: The library was constructed by Claire Murphy, Brandi Chiapelli, and Dr. James McCarter at Washington University, St. Louis. Oligo(dT)-SL1 PCR based library. Parastrongyloides trichosuri parasitic adult cDNA PCR products of size >400 nucleotides containing SL1 on the 5' end and oligo(dT) on the 3' end were non-directionally cloned into pCRII-TOPO(Invitrogen) following the Topo TA cloning protocol. Nematodes were provided by Dr. Warwick Grant of AgRearch, New Zealand (warwick.grant@agresearch.co.nz). Worms were harvested from Australian brush-tailed possum (Trichosuri vulpecula) and washed thoroughly to remove host contamination. Note that despite this effort, host contamination of the library is possible.

text: Parasitic adults

characteristic: dev-stage

characteristic: lab-host

text: Parastrongyloides trichosuri

text: EcoRI

text: Parastrongyloides trichosuri PA SL1 TOPO v1 Murphy Chiapelli McCarter

text: plasmid (kanamycin and ampicillin resistant)

text: pCRII-TOPO (Invitrogen)


characteristic: INSDC last update

text: live

text: Generic

text: The library was constructed by Claire Murphy, Brandi Chiapelli, and Dr. James McCarter at Washington University, St. Louis. Oligo(dT)-SL1 PCR based library. Ascaris suum female gonad MZ cDNA PCR products of size >400 nucleotides containing SL1 on the 5' end and oligo(dT) on the 3' end were non-directionally cloned into pCRII-TOPO(Invitrogen) following the Topo TA cloning protocol. Dissected nematode tissues were provided by Dr. Alan Scott (ascott@jhsph.edu) of the School of Public Hygiene and Public Health at John Hopkins University in Baltimore, MD

text: Adult

text: Ascaris suum

characteristic: organism

text: EcoRI

text: EcoRI

text: Female

text: Dissected female gonad (MZ=maturation zone, most proximal region of gonad

text: Ascaris suum female gonad MZ SL1 TOPO v1

text: pCRII-TOPO (Invitrogen)


text: live

text: Generic

text: The library was constructed by Claire Murphy, Brandi Chiapelli, and Dr. James McCarter at Washington University, St. Louis. Oligo(dT)-SL1 PCR based library. Ascaris suum female intestine cDNA PCR products of size >400 nucleotides containing SL1 on the 5' end and oligo(dT) on the 3' end were non-directionally cloned into pCRII-TOPO(Invitrogen) following the Topo TA cloning protocol. Dissected nematode tissues were provided by Dr. Alan Scott (ascott@jhsph.edu) of the School of Public Hygiene and Public Health at John Hopkins University in Baltimore, MD.

text: Adult

text: Ascaris suum

text: EcoRI

text: EcoRI

text: Female

text: Intestine

text: Ascaris suum female intestine SL1 TOPO v1

characteristic: title

text: pCRII-TOPO (Invitrogen)


text: University of Minnesota

text: live

text: Generic

text: murine-murine hybridoma

text: Library constructed using PCR-Select cDNA subtraction kit (Clontech, Palo Alto, CA) from serum-free suspension culture of murine-murine hybridoma cells

text: Mus musculus

text: Subtracted murine-murine hybridoma cDNA library


text: live

text: Generic

text: Williams 82

characteristic: cultivar

text: The mRNA was prepared using polyatract mRNA system from PROMEGA. The cDNA was prepared using the STRATAGENE kit. Complementary DNA was synthesized from mRNA using a primer consisting of a poly(dT) sequence with a XhoI restriction site (5'GAGAGAGAGAGAGAGAGAGAACTAGTCTCGAGTTTTTTTTTTTTTTTTTT). EcoRI adapters (5'OH-AATTCGGCACGAG and 3'GCCGTGCTCp) were ligated to the blunt-ended cDNA fragments followed by XhoI digestion. The cDNA fragments were directionally cloned into the EcoRI-XhoI restriction sites of the pBluescript vector. The vector was previously dephosphorylated. The ligated cDNA fragments were transformed into DH10B by electroporation. Library is in LB medium with ampicillin and glycerol 8%. Average insert size: 800 bp.

text: Glycine max

text: EcoRI

text: XhoI

text: Roots

characteristic: tissue-type

text: plasmid

text: pBluescript II SK+


text: live

text: Generic

text: First strand cDNA synthesis was primed using oligo-dT on magnetic beads with an additional primer 5'-gcggccgctaatacgactcacta-taggg-3'. Second strand synthesis was a 3-cycle PCR using the primers 5'-ggccgctaatacgactcactatag-3' and 5' -aagcagtggtaacaacgcagagtacttt-ttttttttttttvn-3'. cDNA was subsequently amplified in a 7-cycle PCR with the following primers: 5'-ggccgctaatacgactcactatag-3' and 5'-aagcagtggt-aacaacgcag. Deoxy-UMP adaptors were added in a third PCR (5 cycles) and the primers 5'-caucaucaucauggccgctaatacgactcactataggg-3' and 5'-cuacuacuacuaaagcagtggtaacaacgcagagtac-3'. Ends were treated with uracil DNA glycosylase and product with 3' overhangs was annealed to complementary ends of pAMP1. Insert can be excised using EcoRI and NotI. Library constructed by Joe Barnes and Steve Johnson (Washington University).

text: Danio rerio

text: EcoRI

text: NotI

text: SJD

characteristic: strain

text: whole embryo, 15 day

text: Zebrafish SJD 15 day embryo

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: First strand cDNA synthesis was primed using oligo-dT on magnetic beads with an additional primer 5'-gcggccgctaatacgactcacta-taggg-3'. Second strand synthesis was a 3-cycle PCR using the primers 5'-ggccgctaatacgactcactatag-3' and 5'-aagcagtggtaacaacgcagagtacttt-ttttttttttttvn-3'. cDNA was subsequently amplified in a 7-cycle PCR with the following primers: 5'-ggccgctaatacgactcactatag-3' and 5'-aagcagtggt-aacaacgcag. Deoxy-UMP adaptors were added in a third PCR (5 cycles) and the primers 5'-caucaucaucauggccgctaatacgactcactataggg-3' and 5'-cuacuacuacuaaagcagtggtaacaacgcagagtac-3'. Ends were treated with uracil DNA glycosylase and product with 3' overhangs was annealed to complementary ends of pAMP1. Insert can be excised using EcoRI and NotI. Library constructed by Joe Barnes and Steve Johnson (Washington University).

characteristic: description

text: Danio rerio

text: EcoRI

text: NotI

text: SJD

text: whole embryo, 5 day

text: Zebrafish SJD 5 day embryo

text: phagemid (ampicillin resistant)


characteristic: External Id

text: live

text: Generic

text: Danio rerio

text: EcoRI

text: NotI

text: SJD

text: whole embryo, 2 day

text: Zebrafish SJD 2 day embryo

text: phagemid (ampicillin resistant)


text: University of Sheffield, Medical School

characteristic: INSDC center name

text: live

text: Generic

text: 1st strand cDNA was prepared from day 9.0 embryo cultured with retinol-binding protein antisense oligodeoxynucleotides for 12 hours. An mRNA differential display was performed to isolate the cDNA fragment. Double strand cDNA was cloned into the pPCR-Script Amp SK (+) vector.

text: Embryonic day 9.0 post-fertilization

text: Mus musculus

text: Whole embryo

text: Mus musculus day 9.0 embryo mRNA

text: pPCR script Amp SK (+) (Stratagene)


text: Graduate School of Science, University of Tokyo; CREST, Japan Science and Technology Corporation, Japan

characteristic: INSDC first public

text: live

text: Generic

text: late gastrula to early neurula stages 12.5-13

text: Xenopus laevis

text: anterior endomesoderm

text: Shibata Xenopus AEM lambda-ZAP II cDNA library


text: Harvard University, Howard Hughes Medical Institute

text: live

text: Generic

text: Library constructed using SuperScript Plasmid Library kit (Life Technologies). cDNA made by oligo-dT priming. Size-selected by column fractionation; average insert size 0.97 kb. Amplified once on solid support. cDNA Library Preparation: Guolin Chen.

text: Embryonic day E16.5

text: Mus musculus

text: Not 1

text: Sal 1

text: Both

characteristic: sex

text: pancreas

text: Melton Amplified Mouse E16 5 Pancreas 3 M16S1 A

text: plasmid (ampicillin resistant)


text: Celera Genomics

text: live

characteristic: INSDC status

text: Generic

text: Whole adult mosquitoes (mixed sex) frozen on liquid nitrogen 24 hours after human blood feeding. cDNA inserts >500 bp cloned directionally into pSport 1. Not 1 site is 3'. Clones available through the Malaria Research and Reference Reagent Resource Center (www.malaria.mr4.org)

text: Adult

text: Anopheles gambiae

text: RSP-ST (Reduced susc. to Permethrin - std. chromosome)

text: Plasmid


text: Celera Genomics

text: live

text: Generic

characteristic: NCBI submission model

text: Whole adult mosquitoes (mixed sex) frozen on liquid nitrogen. cDNA inserts >500 bp cloned directionally into pSport 1. Not 1 site is 3'. Clones available through the Malaria Research and Reference Reagent Resource Center (www.malaria.mr4.org).

text: Adult

text: Anopheles gambiae

text: Plasmid


text: University of Dallas

text: live

text: Generic

text: Procambarus clarkii

text: Central Nervous System

text: Crayfish CNS cDNA


text: live

text: Generic

text: mRNA was prepared from antennae of late pupal and newly eclosed male Manduca sexta moths. The mRNA was reverse transcribed with an oligo-dT primer linked to a NotI site and unique tag (TCGGT). An adaptor with an EcoRI site and linker (CATTGTGTTGG) was ligated to the 5' end. The cDNA was cloned into the EcoRI and NotI sites of pGEM-11Zf(+). The library was prepared and randomly sequenced by the W.M. Keck Center for Comparative and Functional Genomics at the University of Illinois at Urbana-Champaign.

text: newly eclosed adults and pharate adults

text: antennae

characteristic: organ

text: Manduca sexta

text: EcoRI

text: NotI

text: male

text: Manduca sexta male antennae pGEM-11Zf library

text: plasmid

characteristic: vector


text: Beth Israel Deaconess Medical Center, Harvard Medical School

text: live

text: Generic

characteristic: NCBI submission package

text: cardiac myocyte

characteristic: cell type

text: cDNA clones from cDNA library made from individual cardiac pools of total RNA isolated from highly homogeneous ventricular cardiac muscle cells population from 15 months old female mice. Cardiomyocytes were isolated by perfusion of the heart.

text: heart

text: Mus musculus

text: EcoRI

text: EcoRI

text: female

text: cardiac muscle

text: Mouse, 15 months old females, left ventricular cardiac muscle cells cDNA library

text: plasmid

text: pCR4Blunt-TOPO (Invitrogen)


characteristic: INSDC last update

text: live

text: Generic

text: cardiac myocyte

text: cDNA clones from cDNA library made from individual cardiac pools of total RNA isolated from highly homogeneous ventricular cardiac muscle cells population from 15 months old male mice. Cardiomyocytes were isolated by perfusion of the heart.

text: heart

text: Mus musculus

text: EcoRI

text: EcoRI

text: female

text: cardiac muscle

text: Mouse, 15 months old males, left ventricular cardiac muscle cells cDNA library

text: plasmid

text: pCR4Blunt-TOPO (Invitrogen)


text: live

text: Generic

text: cardiac myocyte

text: cDNA clones from cDNA library made from individual cardiac pools of total RNA isolated from highly homogeneous ventricular cardiac muscle cells population from 4 months old male mice. Cardiomyocytes were isolated by perfusion of the heart.

text: heart

text: Mus musculus

characteristic: organism

text: EcoRI

text: EcoRI

text: female

text: cardiac muscle

text: Mouse, 4 months old male, left ventricular cardiac muscle cells cDNA library

text: plasmid

text: pCR4Blunt-TOPO (Invitrogen)


text: live

text: Generic

text: cardiac myocyte

text: cDNA clones from cDNA library made from individual cardiac pools of total RNA isolated from highly homogeneous ventricular cardiac muscle cells population from 4 months old female mice. Cardiomyocytes were isolated by perfusion of the heart.

text: heart

text: Mus musculus

text: EcoRI

text: EcoRI

text: female

text: cardiac muscle

text: Mouse, 4 months old female, left ventricular cardiac muscle cells cDNA library

characteristic: title

text: plasmid

text: pCR4Blunt-TOPO (Invitrogen)


text: Texas A&M University

text: live

text: Generic

text: sequence 5' of the insert (5'-NNN...NNNinsert) GCGAATTGGAGCTCCACCGCGGTGGCGGCCGCGGCTCGAG. Sequence 3' of the inserts (AAGAATTCGATATCAAGCTTATCGATACCGTCGACCTCGAG. non-normalized library, sequenced 3' with M13R primer.

text: fetal

characteristic: dev-stage

text: brain

text: Sus scrofa

text: NotI

text: EcoRI

text: CSEQFXL36 fetal brain

text: Plasmid

text: pBluescript SK+


text: Texas A&M University

text: live

text: Generic

text: kidney

text: Sus scrofa

text: NotI

text: EcoRI

text: CSEQFXL12 pig kidney

text: Plasmid

text: pBluescript SK+


text: Texas A&M University

text: live

text: Generic

text: liver

text: Sus scrofa

text: NotI

text: EcoRI

text: CSEQFXL11 pig liver

text: Plasmid

text: pBluescript SK+


text: Texas A&M University

text: live

text: Generic

text: ovary

text: Sus scrofa

text: NotI

text: EcoRI

text: CSEQFXL24 pig ovary

text: Plasmid

text: pBluescript SK+


text: Texas A&M University

text: live

text: Generic

text: brain

text: Sus scrofa

text: NotI

text: EcoRI

text: pituitary gland

characteristic: tissue-type

text: CSEQFXL39 pig pituitary

text: Plasmid

text: pBluescript SK+


text: Texas A&M University

text: live

text: Generic

text: thymus

text: Sus scrofa

text: NotI

text: EcoRI

text: CSEQFXL40 pig thymus

text: Plasmid

text: pBluescript SK+


text: USDA/ARS

text: live

text: Generic

text: sperm

characteristic: cultivar

text: RNA was isolated from fluorescent-activated cell sorted sperm cells. A cDNA library was constructed by oligo dT priming using the Clontech SMART cDNA synthesis kit. The cDNAs were directionally cloned.

text: Zea mays

text: Zea mays sperm cell cDNA library

text: Clontech Triplex2


text: Children's Memorial Research Center

text: live

text: Generic

text: UI-E-DW0 is a cDNA library containing the following tissue(s): lens. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CGATTAGCGA. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI).

characteristic: description

text: adult

text: DH10B (Life Technologies) (T1 phage resistant)

characteristic: lab-host

text: eye

text: Homo sapiens

text: EcoR I

text: Not I

text: lens

text: Plasmid

text: pT7T3-Pac (Pharmacia) with a modified polylinker


characteristic: External Id

text: Children's Memorial Research Center

characteristic: INSDC center name

text: live

text: Generic

text: UI-E-CR0 is a cDNA library containing the following tissue(s): eye anterior segment. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is AATGCCGCAT. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI).

text: adult

text: eye

text: Homo sapiens

text: EcoR I

text: Not I

text: eye anterior segment

text: Plasmid


text: Children's Memorial Research Center

characteristic: INSDC first public

text: live

text: Generic

text: UI-E-EO1 is a normalized cDNA library containing the following tissue(s): fetal eye. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CGCGTATACC. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI).

text: fetal

text: eye

text: Homo sapiens

text: EcoR I

text: Not I

text: fetal eye

text: Plasmid


text: Children's Memorial Research Center

text: live

characteristic: INSDC status

text: Generic

text: UI-E-EO0 is a cDNA library containing the following tissue(s): fetal eye. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CGCGTATACC. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI).

text: fetal

text: eye

text: Homo sapiens

text: EcoR I

text: Not I

text: fetal eye

text: Plasmid


text: Children's Memorial Research Center

text: live

text: Generic

characteristic: NCBI submission model

text: UI-E-DX0 is a cDNA library containing the following tissue(s): fetal eyes. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is AGAATCAAGA. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI).

text: fetal

text: eye

text: Homo sapiens

text: EcoR I

text: Not I

text: fetal eyes

text: Plasmid


text: Children's Memorial Research Center

text: live

text: Generic

text: UI-E-DX1 is a normalized cDNA library containing the following tissue(s): fetal eyes. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is AGAATCAAGA. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI).

text: fetal

text: eye

characteristic: organ

text: Homo sapiens

text: EcoR I

text: Not I

text: fetal eyes

text: Plasmid


text: Children's Memorial Research Center

text: live

text: Generic

text: UI-E-CQ0 is a cDNA library containing the following tissue(s): optic nerve. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CCATTAAGTG. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI).

text: adult

text: eye

text: Homo sapiens

text: EcoR I

text: Not I

text: optic nerve

text: Plasmid

characteristic: vector


text: Children's Memorial Research Center

text: live

text: Generic

characteristic: NCBI submission package

text: UI-E-CL0 is a cDNA library containing the following tissue(s): retina. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CCGCG. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI).

text: adult

text: eye

text: Homo sapiens

text: EcoR I

text: Not I

text: human retina

text: Plasmid


text: Children's Memorial Research Center

text: live

text: Generic

text: UI-E-CR1 is a normalized cDNA library containing the following tissue(s): eye anterior segment. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is AATGCCGCAT. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI).

text: adult

text: eye

text: Homo sapiens

text: EcoR I

text: Not I

text: eye anterior segment

text: Plasmid


text: Children's Memorial Research Center

text: live

text: Generic

text: UI-E-CK1 is a normalized cDNA library containing the following tissue(s): Retina Foveal and Macular. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is GTCC. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI).

text: adult

text: eye

text: Homo sapiens

text: EcoR I

text: Not I

text: Retina Foveal and Macular

text: Plasmid


text: Children's Memorial Research Center

text: live

text: Generic

text: UI-E-CK0 is a cDNA library containing the following tissue(s): Retina Foveal and Macular. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is GTCC. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI).

text: adult

text: eye

text: Homo sapiens

text: EcoR I

text: Not I

text: Retina Foveal and Macular

text: Plasmid


text: Children's Memorial Research Center

text: live

text: Generic

text: UI-E-CQ1 is a normalized cDNA library containing the following tissue(s): optic nerve. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CCATTAAGTG. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI).

text: adult

text: eye

text: Homo sapiens

text: EcoR I

text: Not I

text: optic nerve

text: Plasmid


text: Children's Memorial Research Center

text: live

text: Generic

text: UI-E-CI1 is a normalized cDNA library containing the following tissue(s): RPE and Choroid. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is ACCTA. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI).

text: adult

text: eye

text: Homo sapiens

text: EcoR I

text: Not I

text: RPE and Choroid

text: Plasmid


text: Children's Memorial Research Center

text: live

text: Generic

text: UI-E-CI0 is a cDNA library containing the following tissue(s): RPE and Choroid. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is ACCTA. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI).

text: adult

text: eye

text: Homo sapiens

text: EcoR I

text: Not I

text: RPE and Choroid

text: Plasmid


text: Children's Memorial Research Center

text: live

text: Generic

text: UI-E-DW1 is a normalized cDNA library containing the following tissue(s): lens. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CGATTAGCGA. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI).

text: adult

text: eye

text: Homo sapiens

text: EcoR I

text: Not I

text: lens

text: Plasmid


text: Children's Memorial Research Center

text: live

text: Generic

text: UI-E-CL1 is a normalized cDNA library containing the following tissue(s): retina. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CCGCG. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI).

text: adult

text: eye

text: Homo sapiens

text: EcoR I

text: Not I

text: human retina

text: Plasmid


text: Children's Memorial Research Center

text: live

text: Generic

text: UI-E-EJ1 is a subtracted cDNA library constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tags for this library are: fetal eyes, AGAATCAAGA; lens, CGATTAGCGA; eye anterior segment, AATGCCGCAT; optic nerve, CCATTAAGTG; retina, CCGCG; Retina Foveal and Macular, GTCC; RPE and Choroid, ACCTA. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI).

text: fetal and adult

text: DH10B (Life Technologies) (T1 phage resistant)

text: eye

text: Homo sapiens

text: EcoR I

text: Not I

text: fetal eyes, lens, eye anterior segment, optic nerve, retina, Retina Foveal and Macular, RPE and Choroid

text: Plasmid


text: Children's Memorial Research Center

text: live

text: Generic

text: UI-E-EJ0 is a subtracted cDNA library constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tags for this library are: fetal eyes, AGAATCAAGA; lens, CGATTAGCGA; eye anterior segment, AATGCCGCAT; optic nerve, CCATTAAGTG; retina, CCGCG; Retina Foveal and Macular, GTCC; RPE and Choroid, ACCTA. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI).

text: fetal and adult

text: eye

text: Homo sapiens

text: EcoR I

text: Not I

text: Plasmid

text: pT7T3-Pac (Pharmacia) with a modified polylinker


text: Anhui Medical University

text: live

text: Generic

text: Schistosoma japonicum

text: mixed

characteristic: sex

text: Chinese strain

characteristic: strain

text: larva worms of Schistosoma japonicum


text: Korea Research Institute of Bioscience & Biotechnology

characteristic: INSDC last update

text: live

text: Generic

text: Lymphoblast-like

characteristic: cell type

characteristic: cell-line

text: The poly (A)+ RNA was dephosphorylated with bacterial alkaline phosphatase (BAP) and then decapped with tabacco acid pyrophosphatase (TAP). The decapped intact mRNA was ligated with DNA-RNA linker including EcoR I site by treatment of T4 RNA ligase and the first strand cDNA was synthesized from oligo dT-selected mRNA by priming with dT-tailed vector. The dT-tailed vector was adjusted to have about 60nt. The cDNA vector was circularized with E. coli DNA ligase after digestion of EcoRI which site is also included in vector. An RNA strand converted to a DNA strand by Okayama-Berg method. The obtained cDNA vectors were used for transformation of competent cells E. coli Top10F' by electroporation method. The cDNA libraries constructed by this method are full-length enriched cDNA library.

text: Stomach

text: Homo sapiens

characteristic: organism

text: EcoRI

text: NotI

text: F

text: Ascites

text: Plasmid

text: pCNS


text: live

text: Generic

text: Lymphoblast-like

text: The poly (A)+ RNA was dephosphorylated with bacterial alkaline phosphatase (BAP) and then decapped with tabacco acid pyrophosphatase (TAP). The decapped intact mRNA was ligated with DNA-RNA linker including EcoR I site by treatment of T4 RNA ligase and the first strand cDNA was synthesized from oligo dT-selected mRNA by priming with dT-tailed vector. The dT-tailed vector was adjusted to have about 60nt. The cDNA vector was circularized with E. coli DNA ligase after digestion of EcoRI which site is also included in vector. An RNA strand converted to a DNA strand by Okayama-Berg method. The obtained cDNA vectors were used for transformation of competent cells E. coli Top10F' by electroporation method. The cDNA libraries constructed by this method are full-length enriched cDNA library. After analyzing and sequencing about 2,000 ~ 3,000 colonies in original cDNA library, the abundant cDNAs were selected and amplified by PCR reaction using vector region primer including T7 promotor as 5' primer and N(dT)14 as 3' primer. The PCR products were used as template for synthesis of biotinylated single stranded RNA by in vitro transcription reaction. The synthesized RNA probes were hybridized with antisense single stranded cDNAs prepared from original liberary and incubated with avidin-gel. After removing DNA-RNA hybrids by centrifuge, the subtracted cDNA libraries were constructed by transformaion of the remaining DNA into competent cells E. coli Top10F' with electroporation method.

text: Stomach

text: Homo sapiens

text: EcoRI

text: NotI

text: F

text: Ascites

characteristic: tissue-type

characteristic: title

text: Plasmid

text: pCNS


text: live

text: Generic

text: Epithelial

text: The poly (A)+ RNA was decapped with tabacco acid pyrophosphatase (TAP) and ligated with DNA-RNA linker including EcoRI site by treatment of T4 RNA ligase. The first strand cDNA was synthesized from oligo dT-selected mRNA by priming with dT-tailed vector. The dT-tailed vector was adjusted to have about 60nt. The cDNA vector was circularized with E. coli DNA ligase after digestion of EcoRI which site is also included in vector. An RNA strand converted to a DNA strand by Okayama-Berg method. The obtained cDNA vectors were used for transformation of competent cells E. coli Top10F' by electroporation method.

characteristic: description

characteristic: lab-host

text: Stomach

text: Homo sapiens

text: EcoRI

text: NotI

text: M

text: Ascites

text: Plasmid

text: pCNS


characteristic: External Id

characteristic: INSDC center name

text: live

text: Generic

text: Epithelial

text: The poly (A)+ RNA was decapped with tabacco acid pyrophosphatase (TAP) and ligated with DNA-RNA linker including EcoRI site by treatment of T4 RNA ligase. The first strand cDNA was synthesized from oligo dT-selected mRNA by priming with dT-tailed vector. The dT-tailed vector was adjusted to have about 60nt. The cDNA vector was circularized with E. coli DNA ligase after digestion of EcoRI which site is also included in vector. An RNA strand converted to a DNA strand by Okayama-Berg method. The obtained cDNA vectors were used for transformation of competent cells E. coli Top10F' by electroporation method. After analyzing and sequencing about 2,000 ~ 3,000 colonies in original cDNA library, the abundant cDNAs were selected and amplified by PCR reaction using vector region primer including T7 promotor as 5' primer and N(dT)14 as 3' primer. The PCR products were used as template for synthesis of biotinylated single stranded RNA by in vitro transcription reaction. The synthesized RNA probes were hybridized with antisense single stranded cDNAs prepared from original liberary and incubated with avidin-gel. After removing DNA-RNA hybrids by centrifuge, the subtracted cDNA libraries were constructed by transformaion of the remaining DNA into competent cells E. coli Top10F' with electroporation method.

text: Stomach

text: Homo sapiens

text: EcoRI

text: NotI

text: M

text: Ascites

text: Plasmid

text: pCNS


characteristic: INSDC first public

text: live

characteristic: INSDC status

text: Generic

text: Lymphoblast-like

text: Stomach

text: Homo sapiens

text: EcoRI

text: NotI

text: F

text: Ascites

text: Plasmid


text: live

text: Generic

characteristic: NCBI submission model

text: Lymphoblast-like

text: Stomach

characteristic: organ

text: Homo sapiens

text: EcoRI

text: NotI

text: F

text: Ascites

text: Plasmid


text: live

text: Generic

characteristic: NCBI submission package

text: Lymphoblast-like

text: Stomach

text: Homo sapiens

text: EcoRI

text: NotI

text: M

text: Stomach

text: Plasmid

characteristic: vector


text: live

text: Generic

text: Lymphoblast-like

text: Stomach

text: Homo sapiens

text: EcoRI

text: NotI

text: M

characteristic: sex

text: Stomach

text: Plasmid


characteristic: INSDC last update

text: live

text: Generic

text: Epithelial

text: Stomach

text: Homo sapiens

text: EcoRI

text: NotI

text: M

text: Stomach

text: Plasmid


text: live

text: Generic

text: Epithelial

characteristic: cell type

text: Stomach

text: Homo sapiens

text: EcoRI

text: NotI

text: M

text: Stomach

text: Plasmid


text: live

text: Generic

text: Scattering floating

characteristic: cell-line

text: Stomach

text: Homo sapiens

text: EcoRI

text: NotI

text: F

text: Ascites

text: Plasmid

text: pCNS


text: live

text: Generic

text: Scattering floating

text: Stomach

text: Homo sapiens

characteristic: organism

text: EcoRI

text: NotI

text: F

text: Ascites

text: Plasmid

text: pCNS


text: live

text: Generic

text: Epithelial

text: Stomach

text: Homo sapiens

text: EcoRI

text: NotI

text: M

text: Stomach

characteristic: tissue-type

text: Plasmid

text: pCNS


text: live

text: Generic

text: Epithelial

text: Stomach

text: Homo sapiens

text: EcoRI

text: NotI

text: M

text: Stomach

characteristic: title

text: Plasmid

text: pCNS


text: live

text: Generic

text: Epithelial

characteristic: description

text: Stomach

text: Homo sapiens

text: EcoRI

text: NotI

text: M

text: Ascites

text: Plasmid

text: pCNS


text: live

text: Generic

text: Epithelial

characteristic: lab-host

text: Stomach

text: Homo sapiens

text: EcoRI

text: NotI

text: M

text: Ascites

text: Plasmid

text: pCNS


characteristic: External Id

text: live

text: Generic

text: Epithelial

text: The poly (A)+ RNA was dephosphorylated with bacterial alkaline phosphatase (BAP) and then decapped with tabacco acid pyrophosphatase (TAP). The decapped intact mRNA was ligated with DNA-RNA linker including SfiI site by treatment of T4 RNA ligase and the first strand cDNA was synthesized with Superscript II using SfiI oligo-dT primer. After first strand synthesis, RNA was degraded by NaOH treatment and cDNA was amplified by PCR reaction. The PCR products were digested with SfiI and cloned into DraIII- digested pME18S-FL3 vector. The obtained cDNA vectors were used for transformation of competent cells E. coli Top10F' by electroporation method. The cDNA libraries constructed by this method are full-length enriched cDNA library.

text: Stomach

text: Homo sapiens

text: XhoI

text: XhoI

text: M

text: Ascites

text: Plasmid


characteristic: INSDC center name

text: live

text: Generic

text: Lymphoblast-like

text: Stomach

text: Homo sapiens

text: XhoI

text: XhoI

text: M

text: Stomach

text: Plasmid


characteristic: INSDC first public

text: live

text: Generic

text: Epithelial

text: Stomach

text: Homo sapiens

text: EcoRI

text: NotI

text: F

text: Lymph node

text: Plasmid

text: pCNS


text: live

characteristic: INSDC status

text: Generic

text: Epithelial

text: Stomach

text: Homo sapiens

text: EcoRI

text: NotI

text: F

text: Lymph node

text: Plasmid

text: pCNS


text: live

text: Generic

characteristic: NCBI submission model

text: Homo sapiens

text: EcoRI

text: NotI

text: myeloma

text: Plasmid

text: pCNS


text: live

text: Generic

text: Homo sapiens

text: EcoRI

text: NotI

text: myeloma

text: Plasmid

text: pCNS


text: live

text: Generic

text: Stomach

characteristic: organ

text: Homo sapiens

text: EcoRI

text: NotI

text: Plasmid


text: live

text: Generic

characteristic: NCBI submission package

text: Stomach

text: Homo sapiens

text: EcoRI

text: NotI

text: Plasmid


text: live

text: Generic

text: Stomach

text: Homo sapiens

text: EcoRI

text: NotI

text: M

text: Plasmid

text: pCNS

characteristic: vector


text: live

text: Generic

text: Stomach

text: Homo sapiens

text: EcoRI

text: NotI

text: F

characteristic: sex

text: Plasmid

text: pCNS


characteristic: INSDC last update

text: live

text: Generic

text: Floating aggregates

text: Stomach

text: Homo sapiens

text: EcoRI

text: NotI

text: F

text: Stomach

text: Plasmid


text: live

text: Generic

text: Floating aggregates

characteristic: cell type

text: Stomach

text: Homo sapiens

text: EcoRI

text: NotI

text: F

text: Stomach

text: Plasmid


text: live

text: Generic

text: Lymphoblast-like

characteristic: cell-line

text: The S22SNU16 library was contributed by the Soares laboratory and it was constructed as described by Bonaldo, M.F., Lennon, G. and Soares, M.B. (1996), Genome Research 6(9): 791-806. RNA was prepared from harvested cells of SNU-16 culture. SNU-16 cell was obtained from Korean Cell Line Bank (KCLB). SNU-16 was established from ascitic fluids of Korean patients by Park J.G. et al. (1990), Cancer Res 50: 2773-2780.

text: Stomach

text: Homo sapiens

text: EcoRI

text: NotI

text: F

text: Ascites

text: Plasmid


text: Washington University School of Medicine

text: live

text: Generic

text: The library was provided by Dr. David Bird at North Carolina State University, Raleigh, NC (david_bird@ncsu.edu). The cDNA was made by using Dynabead oligo-dT priming (Dynal). PCR based library using a modified protocol from the SMART PCR cDNA Synthesis Kit from Clontech. Directionally cloned into the UDG sites of pAMP1.

text: egg

characteristic: dev-stage

text: Meloidogyne incognita

characteristic: organism

text: NotI

text: SalI

text: Meloidogyne incognita egg pAMP1 Bird

text: plasmid (ampicillin resistant)

text: pAMP1 (Gibco)


text: North Carolina State University

text: live

text: Generic

characteristic: cultivar

text: cDNA was prepared from polyA+ enriched RNA. The cDNA was directionally ligated into the Unizap XR vector from Stratagene and packaged using Gigapack III Gold packaging extracts. Plasmids containing cDNA inserts were excised from the recombinant lambda-Zap phage using Ex-assist helper phage and propagated in XLOLR cells.

text: 3 days after infection with Meloidogyne incognita second stage larvae

text: XLOLR

text: Medicago truncatula/Meloidogyne incognita mixed EST library

text: EcoRI

text: XhoI

text: 3 week old roots

characteristic: tissue-type

text: BNIR

characteristic: title

text: phagemid

text: pBluescript SK-


characteristic: External Id

text: University of Illinois

text: live

text: Generic

text: 6 and 10 hour drought stress by placing plants on moist paper (75% rel. humidity) in light

characteristic: description

text: 3 week old

text: Hordeum vulgare subsp. vulgare

text: cv tokak

characteristic: strain

text: Root

text: HC


text: University of Illinois

characteristic: INSDC center name

characteristic: INSDC first public

text: live

characteristic: INSDC status

text: Generic

text: 3 week old

text: Hordeum vulgare subsp. vulgare

text: cv tokak

text: Leaf

text: HB


text: Institute for Plant Biotechnology, University of Stellenbosch

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: chardonnay

text: country=South Africa:Stellenbosch

text: Vitis vinifera

text: grape berry

text: Vitis vinifera grape berry


text: Third Military Medical University

text: live

text: Generic

text: breed=New Zealand White; after alkali burn

text: Oryctolagus cuniculus

text: corneal epithelium

text: Oryctolagus cuniculus corneal epithelium


text: University of Alabama at Birmingham

text: live

text: Generic

text: Gli-transduced RK3E cells

text: Rattus norvegicus

text: Rattus norvegicus Gli-transduced RK3E cells


text: Texas A&M University

text: live

text: Generic

text: mycelium

text: Unidirectional cloning. EcoRI side has T3 primer and predominantly 5' reads. T7 primer on XhoI side of insert. Strain inoculated into complete medium grown for two days at room temperature, 150 rpm, harvested, blended, reinoculated into complete medium 24 h, room temperature, 150 rpm. Sequences were processed by one of two methods. Where a full-length alignment to the M. grisea genome sequence was available, the EST sequence was trimed according to the alignment, otherwise sequence quality was assessed using phredPhrap version 991019 and trimmed according to phd files (0.05) and for vector seqs.

text: Pyricularia grisea

text: EcoRI

text: XhoI

text: Mat1-2 hermaphrodite

characteristic: sex

text: Magnaporthe grisea CM Uni-Zap XR Library

text: Phagemid

text: pBluescriptSK-

characteristic: vector


text: Texas A&M University

characteristic: INSDC last update

text: live

text: Generic

text: conidia

characteristic: cell type

text: Unidirectional cloning. EcoRI side has T3 primer and predominantly 5' reads. T7 primer on XhoI side of insert. Conidial library. Point inoculation of Guy11 at center of oatmeal agar plate. Conidia were harvested after two weeks of growth. Sequences were processed by one of two methods. Where a full-length alignment to the M. grisea genome sequence was available, the EST sequence was trimed according to the alignment, otherwise sequence quality was assessed using phredPhrap version 991019 and trimmed according to phd files (0.05) and for vector seqs.

text: Pyricularia grisea

text: EcoRI

text: XhoI

text: Mat1-2 hermaphrodite

text: Magnaporthe grisea CS Uni-Zap XR Library

text: Phagemid

text: pBluescriptSK-


text: Texas A&M University

text: live

text: Generic

text: mycelium

text: Unidirectional cloning. EcoRI side has T3 primer and predominantly 5' reads. T7 primer on XhoI side of insert. Nitrogen starvation library. Cells were inoculated into minimal medium and grown for two days with shaking (150 rpm) at room temperature. Culture was harvested, blended, inoculated into minimal medium as above for 24 h. Cells were harvested, washed with water and inoculated into minimal medium base lacking nitrogen source for 6 h. Sequences were processed by one of two methods. Where a full-length alignment to the M. grisea genome sequence was available, the EST sequence was trimed according to the alignment, otherwise sequence quality was assessed using phredPhrap version 991019 and trimmed according to phd files (0.05) and for vector seqs.

text: Pyricularia grisea

text: EcoRI

text: XhoI

text: Mat1-2 hermaphrodite

text: Magnaporthe grisea NS Uni-Zap XR Library

text: Phagemid

text: pBluescriptSK-


text: Texas A&M University

text: live

text: Generic

text: mycelium

text: Unidirectional cloning. EcoRI side has T3 primer and predominantly 5' reads. T7 primer on XhoI side of insert. Minimal medium mycelium library. Sequences were processed by one of two methods. Where a full-length alignment to the M. grisea genome sequence was available, the EST sequence was trimed according to the alignment, otherwise sequence quality was assessed using phredPhrap version 991019 and trimmed according to phd files (0.05) and for vector seqs.

text: Pyricularia grisea

text: EcoRI

text: XhoI

text: Mat1-2 hermaphrodite

text: Magnaporthe grisea MY Uni-Zap XR Library

text: Phagemid

text: pBluescriptSK+