text: University of California

text: live

text: Generic

text: Parent Washington Navel

text: In cooperation with Dr. Robert Luck and Dr. Joseph Morse, Department of Entomology, University of California Riverside, navel orange fruit was infested with red scale (Aonidiella aurantii [Maskell]). Dr. Luck's technician, Lisa Forster, maintained colonies of red scale. Dr. Morse's technician, Paul Flores, cleaned and infested the fruit, bagged the fruit after the crawlers had time to settle, then harvested the fruit at the early second instar and late third instar phases of the insect. Claire Federici (Dr. Mikeal Roose's lab) cleaned off most of the insects (some remained firmly attached), cut the peel, then froze and stored at -80oC. The trees used for this set of samples were growing in field 16K of the University of California Citrus Experiment Station. This was the same field from which the tissue was collected for the navel shoot meristems and the peel for citrus thrips, Scirtothrips citri [Moulton]. The trees were planted 12 October 1992. The scion was Parent navel. The trees used for infesting with red scale all had Troyer rootstock. Three different trees were used for each of the two infestations, a total of 6 trees. This experiment did not take place according to the original schedule because the red scale colony became infested with mites, so too few insects were available to infest the fruit on the date originally planned. The plan had been to infest on or about June 30 and August 13. Instead the infestation dates were August 30 and September 27, 2004. Because this took us into the cool weather of fall, the insects applied on the second infestation date took about three times as long to develop to the same stage as the insects applied on the first infestation date. The insects were reared on lemons. Before infesting the fruit on the trees, Paul Flores cleaned the peel and checked to make sure it was not already infested. He placed about 200 crawler stage insects on the fruit using a soft paintbrush. After the crawlers had a day to move around and find a spot to attach, Paul placed a very fine mesh drawstring bag over the fruit to exclude predators and parasitoids of the scale. Bags were left in place for the duration of the field development period and also covered control fruit. Paul Flores infested fruit on 30 August and 1 September 2004 after cleaning the fruit on August 27. On 21 September the first set of red scale infested fruit were sampled. He brought Claire Federici 15 infested fruit and 12 uninfested control fruit. The insects were at the early second instar. The controls had been cleaned and bagged at the same time as inoculated ones, but had no scale introduced. The infestation was heavy, so the insects were not well separated on the fruit. A razor blade was used to slice off the flavedo with about half the thickness of the albedo included. The peel from only the stem half of the fruit was used because that was where the insects were most concentrated. The insects were impossible to wash off without severely damaging the peel; each fruit was washed with water and a sponge or bottlebrush and wiped dry with a paper towel before cutting it, but many of the insects were still intact and were included in the sample. The pulp had color but the fruit rind was still green. The peel from each half fruit was bagged separately in foil packets, and pressed between sheets of dry ice to freeze. All packets were placed in a paper bag with the date and information about 'control' or 'infested' written on the bag, and then placed at -80oC. The second sampling date from the first infestation was 11 October. Paul brought 19 infested and 9 uninfested fruit to Claire. These were all set up on 30 August. The insects were at the late third instar. Mikeal Roose's advice was to prepare three bulks of each, 5 infested and 3 uninfested fruit per bulk. The remaining four infested fruit were infested to a lesser degree and were discarded. Washing was done as before and the peel from each fruit frozen between sheets of aluminum foil pressed between sheets of dry ice. There were quite a lot of adhering insects in the frozen rind. After it was frozen, the peel from 3 uninfested or 5 infested fruit were pooled in a foil packet, then stored at -80oC. The pulp had color but the rind was mostly green. However, there was a little color developing on some fruit around the insects. The third sampling date was 2 December. Paul brought the first fruit from the second infestation, which were set up on September 27 or 28. He brought 15 infested and 10 uninfested fruit. Claire cut and froze them as before, making three pools of 5 infested fruit and three pools of 3 uninfested fruit. The control fruit all had green at the stem end, mostly about 1/4 of the circumference from stem to blossom end, but one fruit was green all over. The infested fruit were almost fully orange, with only a small amount of the surface still green, approximately the size of a quarter coin. Paul said it would be at least a month, probably 6 weeks or even more before the insects reach the late third instar, so this was the final sample for the cDNA library due to time constraints for EST sequencing. Mandal and Fenton (Close lab) purified RNA by the phenol method described in J. Japanese Soc. Hort. Sci. 1996. 64 (4): 809-814, purified poly(A) mRNA using PolyAT Tract mRNA Isolation Kit (Promega), produced a primary cDNA library using a lambda ZAP XR cDNA Synthesis Kit (Stratagene), then mass-excised 1 million pfu from the primary library to produce a phagemid population. The library was made from a mixture of RNA from each of the three treatments such that approximately equal amounts of early second instar and late third instar RNA were used. Phagemids were plated, plasmid DNA purified, cDNA clones archived, and DNA sequences determined bi-directionally using an ABI3730 at the Arizona Genomics Institute (Kim, Kudrna, Collura, Wissotski, Byrne, Stum, Smart, Muller, Wing). Chromatogram files were downloaded by FTP by Close, then processed by Wanamaker (Close lab) using the HarvEST pipeline (http://harvest.ucr.edu) to remove vector and cloning oligo sequences and various contaminants, and to trim to a high quality region. Sequences that retained a phred 17 region of at least 100 bases were assembled, then chimeras were removed following manual inspection of assemblies (Wanamaker, Close, Roose). Sequences that survived all removal steps were submitted to GenBank. Clones from this library are archived at the Arizona Genomics Institute (http://www.genome.arizona.edu/orders/).

characteristic: description

text: 12 year old trees

text: E. coli TJC121

text: Citrus sinensis

text: EcoRI

text: XhoI

text: Flavedo, albedo, some red scale

text: Parent Washington Navel Orange Scale-Infested Rind cDNA Library UCRCS11

text: Phagemid pBluescript SK(-)

text: Lambda Uni-ZAP XR, excised phagemid


status: PUBLIC

characteristic: External Id

text: University of Zurich

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Fidel

tag: attribute

characteristic: cultivar

text: Triticum aestivum

characteristic: organism

text: leaf

characteristic: tissue-type

text: Triticum aestivum cv. Fidel leaf

characteristic: title


text: INRA

text: live

text: Generic

text: Funding for EST sequencing was provided by funds dedicated to J.P.RENARD (BDR, Inra). Funding for construction and subtraction of the cDNA library was provided by USDA ARS contract No 58-1275-2-020 to H. A. Lewin The cDNA library was constructed, normalized and subtracted by Dr. A. Hernandez (W.M. Keck Center, University of Illinois Urbana-Champaign) and S. Degrelle (BDR, Inra) as described in Bonaldo et al.(1996), Genome Research 6, 791-806. BE03 library is a normalized library of embryonic tissues subtracted with 7800 known EST sequences. The double-stranded cDNA was size selected (>450 bp), adaptored with EcoRI adaptors at both ends and digested with NotI. The cDNA was then directionally cloned into EcoRI-NotI digested pGEM11Zf(+) phagemid vector. Insert size was between 450-2200 bp. Tagged oligo-dT was used to identify the origin of the ESTs where possible. (A18)TGCGT = extraembryonic tissue; (A18)TCGGT = 36-day oldfetus; (A18)TGCGA = 64-day old fetus (organs); (A18)TCGGA = 64-day old fetus (body)

characteristic: lab-host

text: Bos taurus

text: embryo (day 36 and day 64), extra-embryonic tissue (day 14 to 25)

text: Normalized and Subtracted bovine embryonic and extraembryonic tissues (bcaz/BE03)

characteristic: vector


text: State Key Laboratory of Biotherapy, West China Hospital, Sichuan University

text: live

text: Generic

text: liver

characteristic: organ

text: Xenopus laevis

text: liver tumor

text: Xenopus liver tumor cDNA library


text: Royal Veterinary and Agricultural University

text: live

text: Generic

text: Sus scrofa

text: Sus scrofa library (Lahbib-Mansais Y)


text: Tokyo Metropolitan University

text: live

text: Generic

text: two-celled embryo

characteristic: dev-stage

text: Zea mays

text: Zea mays two-celled embryo


text: University of Stellenbosch

text: live

text: Generic

text: Golden Delicious

text: Extraction of total RNA from stem tissue using a guanidine thiocynate/cesium chloride ultra-centrifugation protocol. Isolation of poly-A mRNA from total RNA using a Dynabeads(r) mRNA purification kit (Dynal). Double stranded full-length 5' and 3' cDNA was produced using the GeneRacer(tm) Kit (Invitrogen Technologies) and the tissue specific cDNA library was constructed with a Zero Blunt(tm) TOPO(r) PCR cloning kit for sequencing (Invitrogen Technologies).

text: TOP10 E.coli

text: Malus domestica

text: stem

text: Apple stem cDNA library

text: plasmid

text: pCR(r) 4Blunt-TOPO


text: University of Stellenbosch

text: live

text: Generic

text: Golden Delicious

text: Extraction of total RNA from root tissue using a guanidine thiocynate/cesium chloride ultra-centrifugation protocol. Isolation of poly-A mRNA from total RNA using a Dynabeads(r) mRNA purification kit (Dynal). Double stranded full-length 5' and 3' cDNA was produced using the GeneRacer(tm) Kit (Invitrogen Technologies) and the tissue specific cDNA library was constructed with a Zero Blunt(tm) TOPO(r) PCR cloning kit for sequencing (Invitrogen Technologies).

text: TOP10 E.coli

text: Malus domestica

text: root

text: Apple root cDNA library

text: plasmid

text: pCR(r) 4Blunt-TOPO


text: University of Stellenbosch

text: live

text: Generic

text: Golden Delicious

text: Extraction of total RNA from leaf tissue using a guanidine thiocynate/cesium chloride ultra-centrifugation protocol. Isolation of poly-A mRNA from total RNA using a Dynabeads(r) mRNA purification kit (Dynal). Double stranded full-length 5' and 3' cDNA was produced using the GeneRacer(tm) Kit (Invitrogen Technologies) and the tissue specific cDNA library was constructed with a Zero Blunt(tm) TOPO(r) PCR cloning kit for sequencing (Invitrogen Technologies).

text: TOP10 E.coli

text: Malus domestica

text: leaf

text: Apple leaf cDNA library

text: plasmid

text: pCR(r) 4Blunt-TOPO


text: Stanford University School of Medicine

text: live

text: Generic

text: Total and poly A+ RNA was isolated from the indicated stickleback tissue, and a cDNA library was constructed in the Express 1 plasmid vector by Open Biosystems. First strand cDNA synthesis was primed with an 54 bp linker primer containing an oligodT sequence preceeded by a synthetic NotI site (first strand primer: 5'-GACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3'). Following second strand synthesis, cDNAs were made blunt at the end corresponding to the original 5 prime end of mRNA, and cloned directionally into the NotI and EcoRV sites of Express 1. Note that the EcoRV site is typically destroyed in the blunt end cloning, leaving a junction of the form 'xxxATC'(where is ATC is the second half of the EcoRV site, and xxx is derived from the cDNA sequence). A map of the Express 1 vector is available at: http://www.openbiosystems.com/cdna_library_construction_faq.php#8 The primary library was transformed and amplified in DH10B (T1 phage resistant) bacteria. Clones available from Open Biosystems: http://www.openbiosystems.com/stickleback

text: adult

text: DH10B (T1 phage resistant)

text: Gasterosteus aculeatus

text: mixed male and female

characteristic: sex

text: Conner Creek sticklebacks, WA USA

characteristic: strain

text: skin

text: SHGC-CEC

text: Plasmid

text: Express 1


characteristic: External Id

text: Stanford University School of Medicine

text: live

text: Generic

characteristic: description

text: adult

text: DH10B (T1 phage resistant)

text: Gasterosteus aculeatus

text: mixed male and female

text: Conner Creek sticklebacks, WA USA

text: gills

text: SHGC-CFW

text: Plasmid

text: Express 1


text: Stanford University School of Medicine

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: adult

text: DH10B (T1 phage resistant)

text: Gasterosteus aculeatus

text: mixed male and female

text: Conner Creek sticklebacks, WA USA

text: eyes

text: SHGC-CGX

text: Plasmid

text: Express 1


text: Stanford University School of Medicine

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: 21 day old larvae collected at Swarup Stage 30 (J. Embryol. Exp. Morphol 6: 373-383.1958)

text: DH10B (T1 phage resistant)

text: Gasterosteus aculeatus

text: mixed male and female

text: Bitrufjordur marine sticklebacks, Iceland

text: whole larva

text: SHGC-CLJ

text: Plasmid

text: Express 1


text: Stanford University School of Medicine

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: adult

text: DH10B (T1 phage resistant)

text: Gasterosteus aculeatus

text: mixed male and female

text: Conner Creek sticklebacks, WA USA

text: brain

text: SHGC-CNB

text: Plasmid

text: Express 1


text: CRA Istituto Sperimentale per la Frutticoltura

text: live

text: Generic

text: Yumyeong

characteristic: cultivar

text: E. coli

text: Prunus persica

characteristic: organism

text: Mesocarp

text: Peach developing fruit mesocarp Stage S4

text: Plasmid


text: Purdue University

text: live

text: Generic

text: Library was prepared from E. rubrum growing on low water activity medium (AM 33)

text: Aspergillus ruber

text: E. rubrum Hybrizap 2.1 Library

characteristic: title


text: Tianjin Medical University

text: live

text: Generic

text: Rattus norvegicus

text: male

text: W

text: liver

characteristic: tissue-type

text: Rattus norvegicus strain W liver


text: US Department of Agriculture. Agricultural Research Service

text: live

text: Generic

text: Urediniospores

characteristic: cell type

text: 1. Total RNA was isolated from germinating spores of P. pachyrhizi isolate TW 72-1 using the ToTally RNA kit (Ambion, Inc., Austin, TX), and the poly(A)+ mRNA was purified using the OLIGOTEX mRNA purification kit (Qiagen, Valencia, CA). 2. First strand cDNA was primed with a Not I primer-adapter (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. Double-stranded cDNA was ligated to Sal I adapter, digested with NotI and cloned into the pSPORT1 vector pre-cut with NotI and SalI. 4. The ligation mix was transformed into DH10B cells.

text: Germinating urediniospores

characteristic: lab-host

text: Phakopsora pachyrhizi

text: Not I

text: Sal I

text: Taiwan 72-1

text: Phakopsora pachyrhizi germinating urediniospores

text: Plasmid

characteristic: vector


text: INRA

text: live

text: Generic

text: cDNA library from plants grown on 10 mM Nitrate

text: Zea mays

text: Plasmid

text: pTRIPLEX


text: INRA

text: live

text: Generic

text: cDNA library from RDA substraction F50-F93xF50

text: 12 DAP

characteristic: dev-stage

text: kernel

characteristic: organ

text: Zea mays

text: Plasmid

text: pGEM-T


characteristic: External Id

text: INRA

text: live

text: Generic

text: cDNA library from RDA substraction F93xF50-F50

text: 12 DAP

text: kernel

text: Zea mays

text: Plasmid

text: pGEM-T


text: University of Bath

text: live

text: Generic

text: The cassava root cDNA library was constructed using the Stratagene ZAP-cDNA synthesis and cloning kit and is directionally cloned in the Uni-ZAP XR insertion vector, which allows in vivo excision of the pBluescript phagemid.

characteristic: description

text: Manihot esculenta

text: EcoRI

text: XhoI

text: Root

text: Cassava root cDNA library

text: phagemid

text: pBluescript SK


text: The Canadian Potato Genome Project - BioAtlantech

characteristic: INSDC center name

text: live

text: Generic

text: Shepody

text: supplier: Plants from pathogen-free Solanum tuberosum var. Shepody, clone 1756, nuclear stock were grown in a screenhouse under natural conditions. Stolons and mature tubers were used for total RNA isolations. Two hundred and fifty nanograms of each sample were pooled and used for full length 1st strand cDNA synthesis with a modified SMART cDNA kit (BD Biosciences), in which the kit CDS III primer was redesigned to contain an XhoI site. This was followed by PCR using the redesigned CDS III primer and the kit 5' PCR primer. PCR products were blunted, and ligated to EcoRI adapters (Invitrogen), followed by XhoI digestion. The double stranded, digested cDNA was gel-purified, and the fraction above 650 bp was collected and ligated with the vector.

text: Solanum tuberosum

text: EcoRI

text: XhoI

text: Combined Stolon and Mature Tuber

text: Full Length Mixed Tuber

text: pBluescript II SK(+) XR


text: Genoplante

characteristic: INSDC first public

text: live

text: Generic

text: Zea mays

text: kernel


text: Huazhong Agricultural University

characteristic: INSDC last update

text: live

text: Generic

text: embryogenic cell

text: Somatic embryogenesis initiation

text: Gossypium hirsutum

text: callus

text: Cotton somatic embryogenesis subtracted cDNA library


text: University of Waterloo

text: live

characteristic: INSDC status

text: Generic

text: Yukon

text: Library was prepared following the protocol for Invitrogen SuperScript Plasmid System with GAteway Technology for cDNA synthesis and cloning

text: 4-week old plants

text: Eutrema salsugineum

text: Leave

text: Drought Library

text: plasmid


text: University of Waterloo

text: live

text: Generic

characteristic: NCBI submission model

text: Yukon

text: Library was prepared following the protocol for BD Clontech PCR-Select cDNA Subtraction Kit and Promega pGEM-T Easy Vector System for cDNA synthesis and cloning

text: 4-week old plants

text: Eutrema salsugineum

text: Leave

text: Cold Subtracted Library

text: plasmid


text: University of Waterloo

text: live

text: Generic

characteristic: NCBI submission package

text: Yukon

text: 4-week old plants

text: Eutrema salsugineum

text: Leave

text: Drought Subtracted Library

text: plasmid


text: University of Waterloo

text: live

text: Generic

text: Yukon

characteristic: cultivar

text: 4-week old plants

text: Eutrema salsugineum

text: Leave

text: Salinity Library

text: plasmid


text: University of Waterloo

text: live

text: Generic

text: Yukon

text: 4-week old plants

text: Eutrema salsugineum

characteristic: organism

text: Leave

text: Salinity Subtracted Library

text: plasmid


text: USDA/ARS

text: live

text: Generic

text: Cotyledons were harvested from 72 hr etiolated seedlings

text: E. coli

text: cotyledon

text: Gossypium hirsutum

text: cDNA from Cotyledons

characteristic: title

text: BlueScript SK


text: Cheng Du Diao Pharmaceutical Group Co., Ltd

text: live

text: Generic

text: 3- and 1-year-old

text: Dioscorea nipponica

text: root

characteristic: tissue-type

text: subtracted cDNA library of Dioscorea nipponica

text: Plasmid

text: pGEM-T


text: University of St Andrews

text: live

text: Generic

text: The library was prepared by Jialin Bai from the Fish Muscle Research Group (University of St Andrews, UK)

text: Juvenile

text: Hippoglossus hippoglossus

text: EcoRI

text: XhoI

text: Fast muscle

text: Fast muscle from juvenile (420 g) Atlantic halibut

text: plasmid

text: pBlueScript II SK(+) XR


text: University of St Andrews

text: live

text: Generic

text: The library was prepared by Dr. Jialin Bai from Fish Muscle Research Group, Gatty Marine Laboratory, School of Biology, University of St Andrews.

text: Yolk-sac Stage Larvae

text: Hippoglossus hippoglossus

text: EcoR I

text: Xho I

text: Skeletal Muscle

text: Trunk of Yolk-sac Stage Atlantic Halibut Larvae

text: Plasmid

text: pBlueScript II SK(+) XR


text: Kunming Institute of Zoology, Chinese Academy of Sciences (CAS)

text: live

text: Generic

text: 4 years old

text: brain

text: Macaca mulatta

text: male

characteristic: sex

text: prefrontal cortex (PFC)

text: cDNA library of rhesus monkey brain PFC


text: Huazhong Agriculture University

text: live

text: Generic

text: SMART cDNA Library(BD Biosciences)

text: Sclerotinia sclerotiorum

characteristic: strain

text: hypha

text: S.sclerotiorum lambda phage ESTs Library


text: Zhongshan (SUN YAT-SEN) University

text: live

text: Generic

text: Mas

text: young leaves

text: Musaceae

text: Musa acuminata

text: young leaves

text: Plantain leaves Suppression Subtraction Library

text: Plasmid

text: pMD18-T Vector

characteristic: vector


text: USDA, ARS

text: live

text: Generic

text: Standard library construction protocols from Stratagene cDNA synthesis kit (Cat No. 200401-5) and Uni-ZAP XR vector kit (Cat No. 237211). A high quality EST with at least 150 contiguous bases at Trace Tuner score of 20 or better.

text: mature fruit

characteristic: dev-stage

text: SOLR

characteristic: lab-host

text: fruit

text: Citrus x paradisi

text: EcoRI

text: XhoI

text: flavedo

text: Star Ruby grapefruit temperature-conditioned flavedo cDNA

text: pBluescript II SK+


characteristic: External Id

text: USDA/ARS

text: live

text: Generic

text: paymaster HS26

text: 5 week old drought stressed seedling

text: E. coli

text: seedlings

characteristic: organ

text: Gossypium hirsutum

text: 5 week old drought stressed seedling

text: BlueScript


text: USDA/ARS

characteristic: INSDC center name

text: live

text: Generic

text: Coker 312

text: 8-10 DAA cotton bolls from irrigated plants

characteristic: description

text: E. coli

text: Gossypium hirsutum

text: BlueScript


text: USDA/ARS

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: paymaster HS26

text: 3 and 5 week old seedlings

text: E. coli

text: seedlings

text: Gossypium hirsutum

text: 3 and 5 week old seedlings

text: BlueScript


text: Oxford University

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Streptocarpus rexii

text: Streptocarpus rexii mRNA


text: BC Cancer Agency

text: live

text: Generic

characteristic: NCBI submission package

text: This work was undertaken as part of the Genome Canada Bovine Genome Sequencing Program. Tissue Procurement: Steve Moore cDNA Library Preparation: Steve Moore cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL) DNA Sequencing by: Canada's Michael Smith Genome Sciences Centre Clone distribution: Clone distribution information can be found through the I.M.A.G.E.Consortium/LLNL at: http://image.llnl.gov

text: Calf, 6 months old

text: ElectroMAX DH10B T1 Phage-Resistant Cells

text: Bos taurus

text: XhoI (5' end of cDNA)

text: BamHI (3' end of cDNA)

text: female

text: Crossbred X Angus

text: Peyer's Patch

text: pBS II SK+


text: BC Cancer Agency

text: live

text: Generic

text: Calf, 6 months old

text: XL10-Gold Ultracompetent Cells

text: Bos taurus

characteristic: organism

text: NotI (3' end of cDNA)

text: SalI (5' end of cDNA)

text: female

text: Crossbred x Angus

text: Ileum

text: pCMV SPORT 6.0


text: Inst. for Biochemistry and Cell Biology, Georg-August-University Gottingen

text: live

text: Generic

text: cDNA prepared by oligo-dT priming and cloned unidirectionally. Average insert size 2,0 kb. Primary non-amplified library constructed by Dr. O. Wessely.

text: stage 6

text: XLBlueMRF' (Stratagene)

text: Embryo

text: Xenopus laevis

text: EcoRI

text: XhoI

text: 32-cell stage cDNA expression library

characteristic: title

text: Plasmid

text: pCS2+ vector (D. Turner, Univ. Michigan)


text: live

text: Generic

text: cDNA prepared by oligo-dT priming and cloned unidirectionally. Average insert size 2,1 kb. Primary non-amplified library constructed by Dr. L. Zon (Harvard Medical School, Boston)/Invitrogen

text: stage 11

text: TOP10F' (Invitrogen)

text: Embryo

text: Xenopus laevis

text: EcoRI

text: NotI

text: UV-ventralized gastrula cDNA expression library

text: Plasmid

text: pCDNA3 vector (Invitrogen)


text: live

text: Generic

text: stage 11

text: XLBlueMRF' (Stratagene)

text: Xenopus laevis

text: EcoRI

text: XhoI

text: LiCl-dorsalized gastrula cDNA expression library

text: Plasmid


text: animal sciences group of wageningen UR

text: live

text: Generic

text: a conA stimulated spleen library normalized and subtracted with CEF cells.

text: Gallus gallus

text: spleen

characteristic: tissue-type

text: conA stimulated spleen library

text: plasmid

text: pGEM -T easy


text: Korea University

text: live

text: Generic

text: Geumgangmill

characteristic: cultivar

text: Total RNA was extracted from roots and base of waterlogging-stressed wheat seedlings (from root tips to 1 cm upper parts from the cotyledonary node). Mixture of tissue samples (40 mg from each treatment) obtained each from 0.5, 1, 2, 4, 6, or 8 days after treatment.

text: Triticum aestivum

text: roots

text: WSWR cDNA library


text: University of Delaware

text: live

text: Generic

text: E.coli TOP10 F

text: Gallus gallus

text: Male and Female

characteristic: sex

text: Con A activated splenic T cells

text: Chicken lymphoid library (pat)


text: Washington University

text: live

text: Generic

text: tissues: adrenal gland, bone marrow, brain cerebellum, brain (whole), fetal brain, fetal liver, heart, kidney, liver, lung (whole), placenta prostate, salivary gland, skeletal muscle, testis, thymus, thyroid gland trachea, uterus, spinal cord. First-stranded cDNA made from pooled mRNA sources. PCR product is sequencing directly, typically from the PCR primers.

text: Homo sapiens

text: pooled


text: Khon Kaen University

text: live

text: Generic

text: adult

text: Opisthorchis viverrini

text: hermaphrodite

text: Khon Kaen Province, Thailand isolate

characteristic: strain

text: whole body

text: Opisthorchis viverrini lambda TriplEx2 Library


text: Tokyo University of Agriculture and Technology

text: live

text: Generic

text: Violet

text: Ipomoea nil

text: corolla

text: Ipomoea nil corolla


text: Chiba Cancer Center Research Institute

text: live

text: Generic

text: Homo sapiens

text: neuroblastoma

text: human 4S neuroblastoma cDNA


text: School of Forestry Northeast Forestry University

text: live

text: Generic

text: Polygonatum sibiricum

text: stem

text: Polygonatum sibiricum suppression subtractive hybridization cDNA library


text: Lincoln University

text: live

text: Generic

text: cDNA subtraction library from the mycoparasitic interaction between Trichoderma hamatum and Sclerotinia sclerotiorum. The library was prepared from mycelium from both Trichoderma hamatum and Sclerotinia sclerotiorum. The submitted ESTs were shown to have come from Trichoderma hamatum using Southern dot blots.

text: Trichoderma hamatum

text: Trichoderma/Sclerotinia mycoparasitism cDNA


text: Miyagi prefecture Agriculture and Horticulture Research Center

text: live

text: Generic

text: shikinari

text: country: Japan:Miyagi

text: Fragaria x ananassa

text: mature leaf

text: strawberry cv. shikinari leaf cDNA


text: The University of Melbourne

text: live

text: Generic

text: Lilium longiflorum

text: generative cell

text: Lilium longiflorum generative cell


text: Keio University

text: live

text: Generic

text: adult

characteristic: dev-stage

text: Dugesia ryukyuensis

text: XhoI

text: EcoRI

text: whole

text: planarian cDNA library

text: pBluescript SK+

characteristic: vector


characteristic: External Id

text: Purdue University

text: live

text: Generic

text: Library construction was performed according to manufacture's (CLONTECH, Palo Alto, CA) recommended protocol for Creator Smart cDNA Library Construction Kit.

text: Selaginella moellendorffii

text: SfiI

text: SfiI

text: Whole plant

text: Selaginella moellendorffii cDNA library SmoC-1

text: Plasmid

text: pDNR-LIB


text: Norwegian School of Veterinary Science

text: live

text: Generic

text: The library was constructed using the Stratagene PCR cDNA Library Construction Kit (Stratagene Cloning Systems, USA) according to the suppliers protocol. This includes PCR amplification of cDNA with adapter-based primers followed by ligation-independent directional cloning into the vector based on the same adapters (LIC-R and LIC-L).

text: compact morula - expanded blastocyst

text: Bos taurus

text: NRF Norwegian Red

text: 20 flushed preimplantation embryos

text: NVH bovine early embryo cDNA library KECB

text: Plasmid

text: pCMV-PCR


text: Washington University School of Medicine

text: live

text: Generic

text: Caenorhabditis elegans

text: Hermaphrodite

text: whole animal

text: Kohara oligo-capped cDNA library


text: National Eye Institute

text: live

text: Generic

text: RNA was extracted from Zebrafish eye tissue. A directionally cloned cDNA library in the pCMVSPORT6 vector (Invitrogen) was constructed at Bioserve Biotechnology (Laurel MD) essentially following the protocols of the SuperScript Plasmid System, full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. cDNA was cloned in Not I/Sal I sites. EST analysis was performed at the NIH Intramural Sequencing Center (NISC). Analyzed data available through http://neibank.nei.nih.gov.

text: Adult

characteristic: lab-host

text: Eye

characteristic: organ

text: Danio rerio

text: Zebrafish whole eye. Unnormalized (naa)

text: Plasmid


text: National Eye Institute

characteristic: INSDC center name

text: live

text: Generic

text: RNA was extracted from Zebrafish lens tissue. A directionally cloned cDNA library in the pCMVSPORT6 vector (Invitrogen) was constructed at Bioserve Biotechnology (Laurel MD) essentially following the protocols of the SuperScript Plasmid System, full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. cDNA was cloned in Not I/Sal I sites. EST analysis was performed at the NIH Intramural Sequencing Center (NISC). Analyzed data available through http://neibank.nei.nih.gov.

characteristic: description

text: Adult

text: Eye

text: Danio rerio

text: Lens

text: Zebrafish lens. Unnormalized (nab)

text: Plasmid


text: National Eye Institute

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: RNA was extracted from Rabbit cornea tissue. A directionally cloned cDNA library in the pCMVSPORT6 vector (Invitrogen) was constructed at Bioserve Biotechnology (Laurel MD) essentially following the protocols of the SuperScript Plasmid System, full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. cDNA was cloned in Not I/Sal I sites. EST analysis was performed at the NIH Intramural Sequencing Center (NISC). Analyzed data available through http://neibank.nei.nih.gov.

text: Adult

text: Eye

text: Oryctolagus cuniculus

text: cornea

text: Rabbit eye cornea. Unnormalized (naf)

text: Plasmid


text: National Eye Institute

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: RNA was extracted from Rabbit eye minus lens and cornea tissue. A directionally cloned cDNA library in the pCMVSPORT6 vector (Invitrogen) was constructed at Bioserve Biotechnology (Laurel MD) essentially following the protocols of the SuperScript Plasmid System, full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. cDNA was cloned in Not I/Sal I sites. EST analysis was performed at the NIH Intramural Sequencing Center (NISC). Analyzed data available through http://neibank.nei.nih.gov.

text: Adult

text: Eye

text: Oryctolagus cuniculus

text: Eye minus lens and cornea

text: Rabbit eye minus lens and cornea. Unnormalized (nag)

text: Plasmid


text: National Eye Institute

text: live

text: Generic

characteristic: NCBI submission package

text: RNA was extracted from Zebrafish posterior segment tissue (with most retina removed). A directionally cloned cDNA library in the pCMVSPORT6 vector (Invitrogen) was constructed at Bioserve Biotechnology (Laurel MD) essentially following the protocols of the SuperScript Plasmid System, full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. cDNA was cloned in Not I/Sal I sites. EST analysis was performed at the NIH Intramural Sequencing Center (NISC). Analyzed data available through http://neibank.nei.nih.gov.

text: Adult

text: Eye

text: Danio rerio

characteristic: organism

text: Posterior segment

text: Zebrafish Posterior segment. Unnormalized (nao)

text: Plasmid


text: National Eye Institute

text: live

text: Generic

text: RNA was extracted from Zebrafish anterior segment (minus lens) tissue. A directionally cloned cDNA library in the pCMVSPORT6 vector (Invitrogen) was constructed at Bioserve Biotechnology (Laurel MD) essentially following the protocols of the SuperScript Plasmid System, full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. cDNA was cloned in Not I/Sal I sites. EST analysis was performed at the NIH Intramural Sequencing Center (NISC). Analyzed data available through http://neibank.nei.nih.gov.

text: Adult

text: Eye

text: Danio rerio

text: Zebrafish anterior segment (minus lens). Unnormalized (nap)

characteristic: title

text: Plasmid


text: National Eye Institute

text: live

text: Generic

text: RNA was extracted from Zebrafish retina tissue. A directionally cloned cDNA library in the pCMVSPORT6 vector (Invitrogen) was constructed at Bioserve Biotechnology (Laurel MD) essentially following the protocols of the SuperScript Plasmid System, full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. cDNA was cloned in Not I/Sal I sites. EST analysis was performed at the NIH Intramural Sequencing Center (NISC). Analyzed data available through http://neibank.nei.nih.gov.

text: Adult

text: Eye

text: Danio rerio

text: Retina

characteristic: tissue-type

text: Zebrafish retina. Unnormalized (naq)

text: Plasmid


text: Huazhong Agriculture University

text: live

text: Generic

text: isolated using subtracted cDNA library from Large White x Meishan hybrid pigs and Meishan pigs

text: Sus scrofa

text: longissimus muscle

text: Sus scrofa longissimus muscle


text: Michigan State University

text: live

text: Generic

text: Vlaspik

characteristic: cultivar

text: cDNAs were made from a total of 10 grams of combined tissues from developing pickling cucumber fruits. Fruits were harvested and finely ground in liquid nitrogen every two days after pollination for 50 days. Tissue was combined within an interval in equal amounts, and combined in the following interval amounts: 2 to 6 days contributed 1.5 grams of the total combined 10 grams (15%); 8 to 14 days (40%); 16 to 18 days (5%); 22 to 28 days (10%); 30 to 36 days (10%); 38 to 44 days (10%); and 46 to 50 days (10%).

text: 2 days to 50 days post-pollination

text: E. coli XL1-Blue MRF

text: fruit

text: Cucumis sativus

text: fruit

text: Cucumber fruit development cDNA library

text: phagemid

text: UNI-ZAP


text: UCSF Comprehensive Cancer Center

text: live

text: Generic

characteristic: cell-line

text: This library was constructed from MCF7 breast cancer cell line by Evrogen JSC. (http://www.evrogen.com) using their standard procedure. See http://shark.ucsf.edu/~stas/report_MCF7.pdf for details.

text: Homo sapiens

text: female

characteristic: sex

text: Human MCF7 breast cancer cell line near full length normalized library (MCF7_EST)


text: Wageningen University and Research Center

text: live

text: Generic

text: Plant treated with 10 uM ZnSO4. HybriZAP cDNA library.

text: Noccaea caerulescens

text: La Calamine

characteristic: strain

text: roots

text: Thlaspi caerulescens roots cDNA library 10 microM ZnSO4


characteristic: External Id

text: live

text: Generic

text: Noccaea caerulescens

text: La Calamine

text: shoots

text: Thlaspi caerulescens shoots cDNA library 10 microM ZnSO4


text: OriGene Technologies, Inc. ( www.origene.com )

text: live

text: Generic

text: Oligo-dT primed reverse transcription optimized for large and GC rich mRNA transcripts, cDNA size selection, optimized ligation for large inserts into mammalian expression vector, random clones selected for end sequence verification of full-length genes

text: Liver

text: Homo sapiens

text: Xho1/Sal1 compatible end ligatio

text: Liver

text: Human liver, large insert, pCMV expression library

text: Plasmid

characteristic: vector


text: live

text: Generic

text: Larix kaempferi

text: Japanese larch somatic embryogenesis SSH library


text: Toyota Central R&D Labs., Inc.

text: live

text: Generic

text: Kokei 14

text: cold-stressed and non-stressed root library

text: late developmental stage

characteristic: dev-stage

text: Ipomoea batatas

text: fibrous root

text: sweetpotato fibrous root cDNA


text: Miyagi prefecture Agriculture and Horticulture Research Center

characteristic: INSDC center name

text: live

text: Generic

text: pechika

text: country: Japan:Miyagi

text: mature

text: Fragaria x ananassa

text: mature leaf

text: strawberry cv. pechika leaf cDNA


text: live

text: Generic

text: pechika mutant. country: Japan:Miyagi

characteristic: description

text: mature

text: Fragaria x ananassa

text: mature leaf

text: strawberry pechika mutant leaf cDNA


text: Nagoya University

characteristic: INSDC first public

text: live

text: Generic

text: forma_specialis: melonis

text: Fusarium oxysporum f. sp. melonis

text: conidial cDNA library


text: Nagoya University

characteristic: INSDC last update

text: live

text: Generic

text: forma_specialis: melonis

text: Fusarium oxysporum f. sp. melonis

text: mycelial cDNA library


text: Nagoya University

text: live

characteristic: INSDC status

text: Generic

text: strawberry pathotype

text: Alternaria alternata

text: Alternaria alternata NAF8


text: University of Padova

text: live

text: Generic

characteristic: NCBI submission model

text: country: Italy:Veneto

text: Cichorium intybus

text: flower

text: Cichorium intybus flower


text: Institute of Biochemical Plant Pathology, GSF

text: live

text: Generic

characteristic: NCBI submission package

text: Barke

text: Hordeum vulgare subsp. vulgare

text: mercuric ion treated root

text: Hordeum vulgare subsp. vulgare mercuric ion treated root


text: USDA, Agricultural Research Service

text: live

text: Generic

text: 9621-67 (Pierce's disease resistant) genotype

text: SSH cDNA library of V. rupestris x V. arizonica hybrid accession # 9621-67 (Pierce's disease resistant genotype) from xylella fastidiosa infected leaf tissue. mRNA was isolated from total RNA and converted into cDNA for library construction according to Clontech PCR-Select cDNA subtraction kit (Cat. #K1804-1, BD Biosciences).

text: vegetative stage infected with Xylella fastidiosa

text: Vitis arizonica x Vitis rupestris

characteristic: organism

text: Rsa-I

text: Leaf

text: cv. 9621-67 infected leaf SSH cDNA library

text: Plasmid

text: pGEM-T Easy vector


text: USDA, Agricultural Research Service

text: live

text: Generic

text: SSH cDNA library of V. rupestris x V. arizonica hybrid accession # 9621-67 (Pierce's disease resistant genotype) from xylella fastidiosa infected stem tissue. mRNA was isolated from total RNA and converted into cDNA for library construction according to Clontech PCR-Select cDNA subtraction kit (Cat. #K1804-1, BD Biosciences).

text: Vitis arizonica x Vitis rupestris

text: Rsa-I

text: Stem

text: cv. 9621-67 infected stem SSH cDNA library

characteristic: title

text: Plasmid

text: pGEM-T Easy vector


text: USDA, Agricultural Research Service

text: live

text: Generic

text: 9621-94 (Pierce's disease susceptible) genotype

text: SSH cDNA library of V. rupestris x V. arizonica hybrid accession # 9621-94 (Pierce's disease susceptible genotype) from xylella fastidiosa infected stem tissue. mRNA was isolated from total RNA and converted into cDNA for library construction according to Clontech PCR-Select cDNA subtraction kit (Cat. #K1804-1, BD Biosciences).

text: Vitis arizonica x Vitis rupestris

text: Rsa-I

text: Stem

characteristic: tissue-type

text: cv. 9621-94 infected stem SSH cDNA library

text: Plasmid

text: pGEM-T Easy vector


text: USDA, Agricultural Research Service

text: live

text: Generic

characteristic: cultivar

text: SSH cDNA library of V. rupestris x V. arizonica hybrid accession # 9621-67 (Pierce's disease resistant genotype) from control leaf tissue. mRNA was isolated from total RNA and converted into cDNA for library construction according to Clontech PCR-Select cDNA subtraction kit (Cat. #K1804-1, BD Biosciences).

text: vegetative stage-control

text: Vitis arizonica x Vitis rupestris

text: Rsa-I

text: Leaf

text: cv. 9621-67 control leaf SSH cDNA library

text: Plasmid

text: pGEM-T Easy vector


text: USDA, Agricultural Research Service

text: live

text: Generic

text: SSH cDNA library of V. rupestris x V. arizonica hybrid accession # 9621-67 (Pierce's disease resistant genotype) from control stem tissue. mRNA was isolated from total RNA and converted into cDNA for library construction according to Clontech PCR-Select cDNA subtraction kit (Cat. #K1804-1, BD Biosciences).

text: vegetative stage-control

text: Vitis arizonica x Vitis rupestris

text: Rsa-I

text: Stem

text: cv. 9621-67 control stem SSH cDNA library

text: Plasmid

text: pGEM-T Easy vector


text: USDA, Agricultural Research Service

text: live

text: Generic

text: SSH cDNA library of V. rupestris x V. arizonica hybrid accession # 9621-94 (Pierce's disease susceptible genotype) from control stem tissue. mRNA was isolated from total RNA and converted into cDNA for library construction according to Clontech PCR-Select cDNA subtraction kit (Cat. #K1804-1, BD Biosciences).

text: vegetative stage-control

text: Vitis arizonica x Vitis rupestris

text: Rsa-I

text: Stem

text: cv. 9621-94 control stem SSH cDNA library

text: Plasmid

text: pGEM-T Easy vector


text: USDA, Agricultural Research Service

text: live

text: Generic

text: SSH cDNA library of V. rupestris x V. arizonica hybrid accession # 9621-94 (Pierce's disease susceptible genotype) from xylella fastidiosa infected leaf tissue. mRNA was isolated from total RNA and converted into cDNA for library construction according to Clontech PCR-Select cDNA subtraction kit (Cat. #K1804-1, BD Biosciences).

text: Vitis arizonica x Vitis rupestris

text: Rsa-I

text: Leaf

text: cv. 9621-94 infected leaf SSH cDNA library

text: Plasmid

text: pGEM-T Easy vector


text: USDA, Agricultural Research Service

text: live

text: Generic

text: SSH cDNA library of V. rupestris x V. arizonica hybrid accession # 9621-94 (Pierce's disease susceptible genotype) from control leaf tissue. mRNA was isolated from total RNA and converted into cDNA for library construction according to Clontech PCR-Select cDNA subtraction kit (Cat. #K1804-1, BD Biosciences).

text: vegetative stage-control

text: Vitis arizonica x Vitis rupestris

text: Rsa-I

text: Leaf

text: cv. 9621-94 control leaf SSH cDNA library

text: Plasmid

text: pGEM-T Easy vector


text: USDA, Agricultural Research Service

text: live

text: Generic

text: 9621-67 (Pierce's disease resistant) genotype

text: SSH cDNA library of V. rupestris x V. arizonica hybrid accession # 9621-67 (Pierce's disease resistant genotype) from xylella fastidiosa infected shoot tissue. mRNA was isolated from total RNA and converted into cDNA for library construction according to Clontech PCR-Select cDNA subtraction kit (Cat. #K1804-1, BD Biosciences).

text: vegetative stage infected with Xylella fastidiosa

text: Vitis arizonica x Vitis rupestris

text: Rsa-I

text: Shoot

text: cv. 9621-67 infected shoot SSH cDNA library

text: Plasmid

text: pGEM-T Easy vector


text: USDA, Agricultural Research Service

text: live

text: Generic

text: 9621-94 (Pierce's disease susceptible) genotype

text: SSH cDNA library of V. rupestris x V. arizonica hybrid accession # 9621-94 (Pierce's disease susceptible genotype) from xylella fastidiosa infected shoot tissue. mRNA was isolated from total RNA and converted into cDNA for library construction according to Clontech PCR-Select cDNA subtraction kit (Cat. #K1804-1, BD Biosciences).

text: Vitis arizonica x Vitis rupestris

text: Rsa-I

text: Shoot

text: cv. 9621-94 infected shoot SSH cDNA library

text: Plasmid

text: pGEM-T Easy vector


text: USDA, Agricultural Research Service

text: live

text: Generic

text: SSH cDNA library of V. rupestris x V. arizonica hybrid accession # 9621-67 (Pierce's disease resistant genotype) from control shoot tissue. mRNA was isolated from total RNA and converted into cDNA for library construction according to Clontech PCR-Select cDNA subtraction kit (Cat. #K1804-1, BD Biosciences).

text: vegetative stage-control

text: Vitis arizonica x Vitis rupestris

text: Rsa-I

text: Shoot

text: cv. 9621-67 control shoot SSH cDNA library

text: Plasmid

text: pGEM-T Easy vector


text: USDA, Agricultural Research Service

text: live

text: Generic

text: SSH cDNA library of V. rupestris x V. arizonica hybrid accession # 9621-94 (Pierce's disease susceptible genotype) from control shoot tissue. mRNA was isolated from total RNA and converted into cDNA for library construction according to Clontech PCR-Select cDNA subtraction kit (Cat. #K1804-1, BD Biosciences).

text: vegetative stage-control

text: Vitis arizonica x Vitis rupestris

text: Rsa-I

text: Shoot

text: cv. 9621-94 control shoot SSH cDNA library

text: Plasmid

text: pGEM-T Easy vector


text: Institute of Vegetables and Flowers of Chinese Academy of Agricultural Sciences

text: live

text: Generic

text: seedlings of three to five leaves

text: Brassica rapa subsp. chinensis

text: Brassica rapa subsp. chinensis seedlings of three to five leaves


text: ARC Seibersdorf Research GesmbH

text: live

text: Generic

text: Smart cDNA library construction kit; Clontech: PT3000-1

text: Lambda

characteristic: lab-host

text: Quercus robur

text: Sfi A

text: Sfi B

text: Tissue culture growing 1 hour in hypertonic medium

text: Oak tissue culture growing 1 hour in hypertonic medium

text: Phage vector


text: ARC Seibersdorf Research GesmbH

text: live

text: Generic

text: Lambda

text: Quercus robur

text: Sfi A

text: Sfi B

text: Tissue culture growing 2 days in hypertonic medium

text: Oak tissue culture growing 2 days in hypertonic medium

text: Phage vector


text: live

text: Generic

text: Total RNA samples were prepared individually from each tissue, checked for quality, then pooled for construction and normalization of the cDNA library by Invitrogen. Plasmids were isolated and inserts end-sequenced by the Marine DNA Sequencing and Analysis Facility at the Center for Marine Functional Genomic Studies, Mount Desert Island Biological Laboratory (J.N. Forrest, Jr., Director). Traces were processed for submission to dbEST by trace2dbest software (Parkinson, Anthony and Blaxter, unpublished software). The project was supported by NIH Grant Number P20-RR-16463 from the INBRE Program of the National Center for Research Resources (P.H. Hand, PI).

text: Adult

text: Fundulus heteroclitus

text: Gill, opercular epithelium, liver, kidney, intestine, brain, muscle; from individual fish acclimated to seawater and freshwater

text: Killifish Multiple Tissue, Normalized

text: pCMV Sport 6.1


text: Sichuan Agricultural University

text: live

text: Generic

text: breed: Abar acer meat

text: newborn

text: Gallus gallus

text: liver

text: Abar acer meat liver newborn


text: Sichuan Agricultural University

text: live

text: Generic

text: breed: SuQin 96 layer

text: newborn

text: Gallus gallus

text: liver

text: SuQin 96 layer liver newborn


text: National Chung Hsing University

text: live

text: Generic

text: 2-year-old Chamaecyparis formosensis seedlings were obtained from the Experimental Forest of National Taiwan University. The developing tissues were frozen in liquid nitrogen. The cDNA library was constructed by pGEM-T Easy vector system (Promega).

text: Chamaecyparis formosensis

text: developing tissue of seedling

text: Chamaecyparis formosensis developing tissue cDNA library

text: pGEM-T Easy


text: Institut National de la Recherche Agronomique

text: live

text: Generic

text: oocyte

characteristic: cell type

text: Subtractive and suppressive hybridization (SSH) between oocyte and somatic tissues (intestine, lung, muscle, cumulus cells)

text: Bos taurus

text: bovine oocyte cDNA SSH library


text: Ocean University of China

text: live

text: Generic

text: gametophyte

text: Gracilariopsis lemaneiformis

text: female

characteristic: sex

text: Gracilaria lemaneiformis gametophyte


characteristic: External Id

text: Ocean University of China

text: live

text: Generic

text: tetra sporophyte

text: Gracilariopsis lemaneiformis

text: Gracilaria lemaneiformis tetra sporophyte


text: Agriculture and Agri-food Canada

text: live

text: Generic

text: urediniospores

text: mRNA obtained by grinding urediniospores & polyA selection; first-strand synthesis by single polyT primer followed by polyG tailing, PCR amplification, restriction with NotI plus Apa1, size-selection and directional cloning in pBlueScriptIIKS. The NotI site represents the 3'-end of the mRNA. PHRED20 sequence clipped for vector sequences. Some clones might have chimeric inserts linked through Apa1 or NotI sites.

text: resting urediniospores

text: E. coli DH10B/r

text: Puccinia triticina

text: NotI

text: race 1 (designatn BBB; Long et al, 1989, Plant Disease 73, 294)

characteristic: strain

text: Plasmid

text: pBSII(KS)

characteristic: vector


text: Agriculture and Agri-food Canada

characteristic: INSDC center name

text: live

text: Generic

text: urediniospores

text: urediniospores germinated for 16 hrs over water

characteristic: dev-stage

text: E. coli DH10B/r

text: Puccinia triticina

text: NotI

text: Plasmid

text: pBSII(KS)


text: Agriculture and Agri-food Canada

characteristic: INSDC first public

text: live

text: Generic

text: mycelium in compatible wheat (Triticum aestivum) cultivar Thatcher

text: mRNA obtained by grinding infected leaf tissue & polyA selection; Stratagene's adapter protocol; directional cloning XhoI - EcoR1 in pBlueScriptIIKS. The XhoI site represents the 3'-end of the mRNA. PHRED20 sequence clipped for vector sequences.

characteristic: description

text: haustorial, 6 days after inoculation

text: E. coli DH10B/r

text: Puccinia triticina

text: XhoI

text: Plasmid

text: pBSII(KS)


text: INRA

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Quercus petraea Liebl.; cDNA-AFLP

text: hypoxic root on one year old seedlings

text: Quercus petraea

text: root

text: Quercus petraea hypoxic root on one year old seedlings


text: University of Birmingham

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: country: United Kingdom

text: Ulva linza

text: sporulating thallus

text: Ulva linza sporulating thallus


text: Humboldt-University of Berlin

text: live

text: Generic

text: sporozoite

text: Eimeria tenella

characteristic: organism

text: Houghton

text: Eimeria tenella Houghton sporozoite

characteristic: title


text: INRA - SCRIBE, Campus de beaulieu

text: live

text: Generic

text: Oncorhynchus mykiss

text: intestine

characteristic: tissue-type

text: Rainbow trout intestine SSH library reverse from salinity stress (rtricc)

text: pT-Adv


text: INRA - SCRIBE, Campus de beaulieu

text: live

text: Generic

characteristic: lab-host

text: Oncorhynchus mykiss

text: intestine

text: Rainbow trout intestine SSH library forward from salinity stress (rtricb)

text: pT-Adv


text: INRA - SCRIBE, Campus de beaulieu

text: live

text: Generic

text: Oncorhynchus mykiss

text: gill

text: Rainbow trout gill SSH library from salinity stress (rtrgg8)

text: pT-Adv


text: INRA - SCRIBE, Campus de beaulieu

text: live

text: Generic

text: Oncorhynchus mykiss

text: gill

text: Rainbow trout gill SSH library reverse from salinity stress (rtrgah)

text: pT-Adv


text: INRA - SCRIBE, Campus de beaulieu

text: live

text: Generic

text: Oncorhynchus mykiss

text: gill

text: Rainbow trout gill SSH library forward from salinity stress (rtrgag)

text: pT-Adv


text: INRA - SCRIBE, Campus de beaulieu

text: live

text: Generic

text: Oncorhynchus mykiss

text: brain

text: Rainbow trout brain SSH library reverse from salinity stress (rtrbaj)

text: pT-Adv


text: INRA - SCRIBE, Campus de beaulieu

text: live

text: Generic

text: Oncorhynchus mykiss

text: head kidney

text: Rainbow trout head kidney SSH library reverse from confinement stress (rtryak)

text: pT-Adv


text: INRA - SCRIBE, Campus de beaulieu

text: live

text: Generic

text: Oncorhynchus mykiss

text: skin

text: Rainbow trout skin SSH library reverse from confinement stress (rtrnam)

text: pT-Adv


text: INRA - SCRIBE, Campus de beaulieu

text: live

text: Generic

text: Oncorhynchus mykiss

text: skin

text: Rainbow trout skin SSH library forward from confinement stress (rtrnan)

text: pT-Adv


text: INRA - SCRIBE, Campus de beaulieu

text: live

text: Generic

text: Oncorhynchus mykiss

text: liver

text: Rainbow trout liver SSH library forward from salinity stress (rtrlbz)

text: pT-Adv


text: INRA - SCRIBE, Campus de beaulieu

text: live

text: Generic

text: Oncorhynchus mykiss

text: liver

text: Rainbow trout liver SSH library reverse from salinity stress (rtrlca)

text: pT-Adv


text: INRA - SCRIBE, Campus de beaulieu

text: live

text: Generic

text: Oncorhynchus mykiss

text: brain

text: Rainbow trout brain SSH library forward from salinity stress (rtrbai)

text: pT-Adv


text: INRA - SCRIBE, Campus de beaulieu

text: live

text: Generic

text: Oncorhynchus mykiss

text: head kidney

text: Rainbow trout head kidney SSH library forward from confinement stress (rtryal)

text: pT-Adv


text: National University of Ireland, Galway

text: live

text: Generic

text: Oncorhynchus mykiss

text: pituitary

text: Pituitary confinement stress library (rtgp)


text: National University of Ireland, Galway

text: live

text: Generic

text: Oncorhynchus mykiss

text: brain

text: Brain confinement stress library (rtgb)


text: National University of Ireland, Galway

text: live

text: Generic

text: Oncorhynchus mykiss

text: liver

text: Liver confinement stress library (rtgl)


text: Universitaet zu Koeln

text: live

text: Generic

text: Scherffelia dubia

text: SAG 40.89

text: Scherffelia dubia SAG 40.89


text: National Taiwan University

text: live

text: Generic

text: 12-year-old Chamaecyparis formosensis inner bark were obtained from the Experimental Forest of National Taiwan University. The inner bark tissues were frozen in liquid nitrogen. The cDNA library was constructed by pGEM-T Easy vector system (Promega).

text: Chamaecyparis formosensis

text: inner bark tissue

text: Chamaecyparis formosensis inner bark tissue cDNA library

text: pGEM-T Easy


text: USDA, ARS

text: live

text: Generic

text: Standard library construction protocols from Stratagene cDNA synthesis kit (Cat No. 200401-5) and Uni-ZAP XR vector kit (Cat No. 237211)

text: mature fruit

text: SOLR

text: fruit

characteristic: organ

text: Citrus x paradisi

text: EcoRI

text: XhoI

text: flavedo

text: Star Ruby grapefruit hot water-treated flavedo

text: pBluescript II SK+


text: Beijing AgroBiotechnology Research Center

text: live

text: Generic

text: longbai NO.2

characteristic: cultivar

text: SSH cDNA library

text: one month old

text: leaves

text: Brassica rapa subsp. pekinensis

text: longbai no.2 one month old leaves

text: plasmid

text: pGEM-T


characteristic: External Id

text: Nan Fang Medical University

text: live

text: Generic

text: Collected the healthy adult sperm, then extracted the total RNAs from them by RNeasy mini kit(QIAGEN). Corresponding cDNAs were produced and sequentially digested into fragments with mean length 256 bp by Sau3AI. Then these fragments linked with a univeral adapter and amplifide by PCR reaction. The products of PCR integrated with T-vector, then transformed into E.Coli. PCR identification reactions were used to find the positive transformant. These positive transformant were amplied for getting the spermatozoal cDNA fragments eventually. Sequencing results were performed afer these fragments were purified.

text: mature sperm

text: Homo sapiens

text: male

characteristic: sex

text: Homo sapiens spermatozoal gene Express Library


text: National Taiwan University

text: live

text: Generic

text: Inner Bark of 25-year-old Taiwania were obtained from the Experimental Forest of National Taiwan University. The tissues of inner bark were frozen in liquid nitrogen. The cDNA library was constructed by pGEM-T Easy vector system (Promega).

text: Taiwania cryptomerioides

text: inner bark

text: Taiwania inner bark cDNA library

text: pGEM-T Easy

characteristic: vector


text: Institute of Himalayan Bioresource Technology

characteristic: INSDC center name

text: live

text: Generic

text: PCR-TRAP cloning system (GenHunter, USA)

text: Camellia sinensis

text: tea bud and first leaf experiencing drought stress

text: Camellia sinensis cv. UPASI-10 tea bud and first leaf

text: PCR-TRAP


text: Cardiff University

text: live

text: Generic

text: Gammarus pulex male subtractive library

text: Adult

characteristic: dev-stage

text: Gammarus pulex

text: Male

text: maS

text: pGEM-T


characteristic: INSDC first public

text: live

text: Generic

text: Euglena gracilis

text: UTEX

characteristic: strain

text: VDSF Euglena gracilis UTEX


text: National Institute of Genetics

characteristic: INSDC last update

text: live

text: Generic

text: varied

text: Misgurnus anguillicaudatus

text: olfactory epithelium

text: Yasufumi Emori unpublished cDNA library, olfactory epithelium


text: Tsinghua University

text: live

characteristic: INSDC status

text: Generic

text: pollen

characteristic: cell type

text: Total RNA was isolated from microspores, germinated and ungerminated pollen using QIAGEN RNAeasy Plant Mini Kit (Qiagen). mRNA was isolated from total RNA with FastTrack 2.0 mRNA Isolation Kit (Invitrogen). From the pooled mRNAs, a cDNA library was constructed using PCR cDNA Library Construction Kit (Stratagene)

characteristic: description

text: Lilium longiflorum

text: Lily pollen cDNA library

text: Lambda phage UNI-ZAP (Stratagene)


text: Sun Yat-sen University School of Life Science

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: ESTs expressed preferentially in amicronucleate cell of Pseudourostyla cristata during stationary phase.

text: stationary

text: Pseudourostyla cristata

characteristic: organism


text: live

text: Generic

text: ESTs expressed preferentially in micronucleate cell of Pseudourostyla cristata during stationary phase.

text: stationary

text: Pseudourostyla cristata

characteristic: title


text: Shanghai Fisheries University

text: live

text: Generic

text: prometamorphosis larva

text: Paralichthys olivaceus

text: head

characteristic: tissue-type

text: Paralichthys olivaceus head prometamorphosis larva


text: Oklahoma State University

text: live

text: Generic

text: MSU

characteristic: cultivar

text: Messenger RNA was extracted from control and cold acclimated bermudagrass crown tissue at 2 and 28 days after acclimation and cDNA library was constructed following Clontech PCR- select cDNA subtraction procedure.

text: E. coli

characteristic: lab-host

text: Cynodon dactylon

text: crown

text: Bermudagrass cv. MSU subtracted cold acclimated cDNA library

text: Qiagen's pDrive


characteristic: External Id

text: Oklahoma State University

text: live

text: Generic

text: Zebra

text: E. coli

text: Cynodon dactylon

text: Crown

text: Bermudagrass line Zebra subtracted cold acclimated cDNA library

text: Qiagen's pDrive


text: OriGene Technologies, Inc. ( www.origene.com )

text: live

text: Generic

text: Oligo-dT primed reverse transcription optimized for large and GC rich mRNA transcripts, cDNA size selection, optimized ligation for large inserts into mammalian expression vector, random clones selected for end sequence verification of full-length genes

text: Brain

characteristic: organ

text: Homo sapiens

text: Xho1/Sal1 compatible end ligatio

text: Whole brain

text: Human adult whole brain, large insert, pCMV expression library

text: Plasmid

characteristic: vector


characteristic: INSDC center name

text: live

text: Generic

text: Homo sapiens

text: Xho1/Sal1 compatible end ligatio

text: Bone marrow

text: Human bone marrow, large insert, pCMV expression library

text: Plasmid


characteristic: INSDC first public

text: live

text: Generic

text: Endothelial cells

text: Homo sapiens

text: Xho1/Sal1 compatible end ligatio

text: Human endothelial cells, large insert, pCMV expression library

text: Plasmid


text: live

text: Generic

text: Oligo-dT primed reverse transcription optimized for large and GC rich mRNA transcripts, cDNA size selection, optimized ligation for large inserts into mammalian expression vector, random clones selected for end sequence verification of full-length genes; constructed and available from OriGene Technologies

text: Heart

text: Homo sapiens

text: Xho1/Sal1 compatible end ligatio

text: Heart

text: Human heart, large insert, pCMV expression library

text: Plasmid


text: live

text: Generic

text: Kidney

text: Homo sapiens

text: Xho1/Sal1 compatible end ligatio

text: Kidney

text: Human kidney, large insert, pCMV expression library

text: Plasmid


text: live

text: Generic

text: Lung

text: Homo sapiens

text: Xho1/Sal1 compatible end ligatio

text: Lung

text: Human lung, large insert, pCMV expression library

text: Plasmid


text: live

text: Generic

text: Primary peripheral blood lymphocytes

text: Homo sapiens

text: Xho1/Sal1 compatible end ligatio

text: Human peripheral blood lymphocytes, large insert, pCMV expression library

text: Plasmid


text: live

text: Generic

text: Homo sapiens

text: Xho1/Sal1 compatible end ligatio

text: Placenta

text: Human placenta, large insert, pCMV expression library

text: Plasmid


text: live

text: Generic

text: Prostate

text: Homo sapiens

text: Xho1/Sal1 compatible end ligatio

text: Prostate

text: Human prostate, large insert, pCMV expression library

text: Plasmid


text: live

text: Generic

text: Spleen

text: Homo sapiens

text: Xho1/Sal1 compatible end ligatio

text: Spleen

text: Human spleen, large insert, pCMV expression library

text: Plasmid


text: live

text: Generic

text: Testis

text: Homo sapiens

text: Xho1/Sal1 compatible end ligatio

text: Testis

text: Human testis, large insert, pCMV expression library

text: Plasmid


text: Northwest A&F University

text: live

text: Generic

text: young leaves

text: Dirrerential displayed sequences. Total RNA was extracted by Trizol, then reverse transcription was conducted by M-MLV reverse transcriptase; then double strand cDNA was synthesized and subjected to cDNA/AFLP analysis by using Licor AFLP EXP ANALYSIS KIT 800; differential displayed bands were cut through the Licor Odyssey Infrared Imaging System, and re-amplified by the original primers; re-amplified bands were cut from agarose gel and purified, then cloned into pMD-18T vector and transformed to E coli strain DH5alpha sequencing were conducted by M13 forward or reverse primer at Bioasia.

text: 5 leaves

characteristic: dev-stage

text: Panicum miliaceum

text: leaves

text: leaves of broom corn millet after drought treatment and recovery from drought


text: Northwest A&F University

characteristic: INSDC last update

text: live

text: Generic

text: young spikelets

text: Dirrerential displayed sequences. Total RNA was extracted by Trizol from spikelets, then reverse transcription was conducted by M-MLV reverse transcriptase; then double strand cDNA was synthesized and subjected to cDNA/AFLP analysis by using Licor AFLP EXP ANALYSIS KIT 800; differential displayed bands were cut through the Licor Odyssey Infrared Imaging System, and re-amplified by the original primers; re-amplified bands were cut from agarose gel and purified, then cloned into pMD-18T vector and transformed to E coli strain DH5alpha sequencing were conducted by M13 forward or reverse primer at Bioasia.

text: after the miosis of Pollen mother cell

text: Triticum aestivum

text: spikelets

text: spikelets of YS thermo-sensitive male sterile wheat line A732 under male sterile and fertile conditions


text: Northwest A&F University

text: live

characteristic: INSDC status

text: Generic

text: leaves

text: Dirrerential displayed sequences. Total RNA was extracted by Trizol, then reverse transcription was conducted by M-MLV reverse transcriptase; then double strand cDNA was synthesized and subjected to cDNA/AFLP analysis by using Licor AFLP EXP ANALYSIS KIT 800; differential displayed bands were cut through the Licor Odyssey Infrared Imaging System, and re-amplified by the original primers; re-amplified bands were cut from agarose gel and purified, then cloned to pMD-18T vector and transformed to E coli strain DH5alpha sequencing were conducted by M13 forward or reverse primer at Bioasia.

text: three-leaf stage, after 2 days inoculation with Stripe rust pathogen

text: Triticum aestivum

text: leaves

text: leaves of stripe rust resistant and susceptible varieties infected by stripe rust


text: Northeast Agricultural University of China

text: live

text: Generic

text: Trifolioate leaf after treated in darkness for 15 days

text: E.coli DH5alpha

text: Glycine max

text: Leaf

text: Soybean leaf substracted cDNA library induced by darkness

text: Plasmid

text: pGEMT


text: School of Medicine of Xian Jiaotong University

text: live

text: Generic

text: transitional cell

characteristic: cell type

characteristic: cell-line

text: A differentially expressed gene subtractive library was constructed by using subtractive suppression hybridization between the pair of bladder transitional cell lines BLZ-211 and BLS with different metastatic potentials. These EST fragments were ligated with pGEM-T vectors and amplified in JM109 E. coli. After blue-white screening, the white positive inserts were picked out and stored.

text: adult

text: bladder

text: Homo sapiens

text: male

characteristic: sex

text: bladder transitional cell carcinoma cell lines subtractive library

text: Plasmid

text: pGEM-T


text: Cardiff University

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: The library was prepared using protocols given by the supplier (Clontech).

characteristic: description

text: Adult

text: Lumbricus rubellus

text: mixed

text: whole worm

text: Earthworm Copper Exposure Library

text: pbluescript II SK


text: Agriculture and Agri-food Canada

text: live

text: Generic

text: 5' ESTs; cDNAs were selected for full-length by the Cap-trapper method and directionally cloned.

text: E. coli DH10B

text: Phytolacca americana

characteristic: organism

text: Sfi I

text: Young leaves from mature plants

characteristic: tissue-type

text: Pa01_AAFC_ECORC_Phytolacca_americana_leaf

characteristic: title

text: Plasmid

text: pHSX-Ci


characteristic: External Id

text: National Institute of Genetics

text: live

text: Generic

text: adult

text: Branchiostoma floridae

text: whole animal

text: Amphioxus Branchiostoma floridae unpublished cDNA library, adult whole animal


text: National Institute of Genetics

text: live

text: Generic

text: egg

text: Branchiostoma floridae

text: whole animal

text: Amphioxus Branchiostoma floridae unpublished cDNA library, egg whole animal


text: National Institute of Genetics

text: live

text: Generic

text: gastrula

text: Branchiostoma floridae

text: whole animal

text: Amphioxus Branchiostoma floridae unpublished cDNA library, gastrula whole animal


text: National Institute of Genetics

text: live

text: Generic

text: larva

text: Branchiostoma floridae

text: whole animal

text: Amphioxus Branchiostoma floridae unpublished cDNA library, larva whole animal


text: National Institute of Genetics

text: live

text: Generic

text: neurula

text: Branchiostoma floridae

text: whole animal

text: Amphioxus Branchiostoma floridae unpublished cDNA library, neurula whole animal


text: Institute of Oil Crops Research

text: live

text: Generic

text: Zhongshuang No.9

characteristic: cultivar

text: developing embryos

text: Brassica napus

text: Brassica napus developing embryos


text: National Institute of Genetics

text: live

text: Generic

text: adult

text: Oryzias latipes

text: female

text: Hd-rR

characteristic: strain

text: whole body

text: MF01ASD cDNA


text: University of Kaiserslautern

characteristic: INSDC center name

text: live

text: Generic

text: haustoria

text: Escherichia coli NM514

characteristic: lab-host

text: Uromyces viciae-fabae

text: EcoRI

text: EcoRI

text: dikaryon

text: haustorium-specific cDNA library

text: Phage

text: lambda gt10

characteristic: vector


text: Louisiana State University Agricultural Center

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Vermilion

text: Salinity induced TDFs identified by cDNA-AFLP using the restriction enzymes, EcoRI and MseI.

text: Vegetative

characteristic: dev-stage

text: Sporobolus alterniflorus

text: Leaves

text: Spartina alterniflora leaf salinity induced transcript-derived fragment (TDF)


text: live

characteristic: INSDC status

text: Generic

text: Vermilion

text: Vegetative

text: Sporobolus alterniflorus

text: Root

text: Spartina alterniflora root salinity induced transcript-derived fragment (TDF)


text: The University of Georgia, Department of Plant Biology

text: live

text: Generic

text: The library was prepared from polyA+ RNA from the roots of 1-year-old loblolly pine (Pinus taeda) cuttings that were rooted and then planted in washed sand. The rooted cuttings were maintained for 50 days (May 2003 harvest) under ambient conditions in a local greenhouse. They were kept on a weekly regimen of 0.5x nutrient-complete Hoagland's solution and supplemented with additional water sufficient to maintain a 15% soil moisture content. Twenty-four hours (24h) prior to harvesting roots for mRNA preparation, the potted trees were placed at 37 C in a growth chamber under a diurnal light cycle. Double-stranded cDNA was cloned unidirectionally into pSL1180. Inserts can be excised with EcoRI (5' end) and XhoI (3' end).

text: DH10B-T1 phage-resistant E. coli

text: Root

characteristic: organ

text: Pinus taeda

text: EcoRI

text: XhoI

text: 3 CCLONES

text: Root at 37 C for 24 hr


text: National Institute of Genetics

text: live

text: Generic

characteristic: NCBI submission model

text: adult

text: Oryzias latipes

text: mixture of female and male

characteristic: sex

text: Hd-rR

text: adult liver

text: MF01ALA cDNA


text: live

text: Generic

characteristic: NCBI submission package

text: The library was prepared from polyA+ RNA isolated from stems of loblolly pine (Pinus taeda) hedges challenged with the necrotrophic fungus Fusarium circinatum, the inciting agent of pitch canker disease. Four pitch canker susceptible hedges were inoculated in November, 2003. For each hedge, multiple succulent shoot tips were inoculated with 1 mL of a 500 spores/mL solution after excision. The terminal 1 cm of each challenged stem was harvested for mRNA isolation 10 days after challenge and bulked.

characteristic: description

text: DH10B-T1 phage-resistant E. coli

text: Shoot tip

text: Pinus taeda

text: EcoRI

text: XhoI

text: 3 CCLONES

text: Shoot tip pitch canker susceptible


text: Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais

text: live

text: Generic

text: sequences obtained by differential display approach of cDNAs

text: adult

text: Canis familiaris

text: Ancylostoma caninum

characteristic: organism

text: Female

text: Ancylostoma caninum female differential display


text: live

text: Generic

text: adult

text: Canis familiaris

text: Ancylostoma caninum

text: male

text: Ancylostoma caninum male differential display

characteristic: title


characteristic: External Id

text: Ocean University of China

text: live

text: Generic

text: Takifugu rubripes

text: ovary

characteristic: tissue-type

text: Takifugu rubripes ovary


text: Ocean University of China

text: live

text: Generic

text: Takifugu rubripes

text: testis

text: Takifugu rubripes testis


text: Ocean University of China

text: live

text: Generic

text: Takifugu rubripes

text: fin

text: Takifugu rubripes fin


text: live

text: Generic

text: Our sequences are ESTs from comparing transcriptome difference in development between Sm1021(wild type) and Sm1354(Tn5::nifA)-induced nodule in Medicago sativa by cDNA-AFLP.

text: Medicago sativa

text: Medicago sativa cDNA-AFLP (Zou and Gong)


text: Alfred-Wegner-Institute for Polar and Marine Research; Institute for Molecular Biotechnology

text: live

text: Generic

text: Samples for total RNA isolation were taken continous for 5 days after a salt shock treatment increasing salinity from 34 to 60 PSU. Total RNA extraktion was performed with RNAqueous (Ambion) and mRNA purification with polyA Purist (Ambion). Further steps were carried out as described in the Cloneminer Kit. cDNA size fractionation was carried out with CHROMA Spin-400 columns and additionally on a gel.

text: Fragilariopsis cylindrus

text: F. cylindrus osmotic stress library


text: Plant Research International

text: live

text: Generic

text: Elsanta

characteristic: cultivar

text: ripe

text: Fragaria x ananassa

text: fruit

text: Strawberry ripe fruit Uni-zapXR cDNA library


text: ARO, The Volcani Center

characteristic: INSDC center name

text: live

text: Generic

text: Citrus x paradisi

text: flavedo

text: Citrus x paradisi flavedo


text: Tulane University, Health Sciences Center

text: live

text: Generic

text: adult

text: Opisthorchis viverrini

text: hermaphrodite

text: Khon Kaen Province, Thailand isolate

characteristic: strain

text: whole body

text: Opisthorchis viverrini pGEM-T Library


text: College of Veterinary Medicine, South China Agricultural University

text: live

text: Generic

text: The library was created by Shaoqiang Wu from the female adult ascaris suum. Sequencing was performed by the Invitrogen Biotechnology Co.,Ltd Ascaris suum, a large roundworm, is a nematode parasite of pigs. In order to identify the genes related to larva migrans, which will cause VLM/OLM of humans, the EST library was constructed from the female adult Ascaris suum cDNA which is subtracted by cDNA of female adult Ascaris lumbricoides using Clontech PCR-Select cDNA Subtraction Kit. The subtracted female adult Ascaris suum cDNA was ligated to pGEM-T easy vector.

text: adult

text: Ascaris suum

text: EcoRI

text: EcoRI

text: Female

text: Ascaris suum adult female Specific Library

text: pGEM-T easy

characteristic: vector


text: Institute for Molecular and Cellular Regulation, Gunma University

characteristic: INSDC first public

text: live

text: Generic

text: Homo sapiens

text: small intestine

text: Homo sapiens small intestine


text: Forestry and Forest Products Institute

characteristic: INSDC last update

text: live

text: Generic

text: This cDNA clone specifically expressed in primordium or mature fruit body of Pleurotus ostreatus.

text: Pleurotus ostreatus

text: fruit body

text: Pleurotus ostreatus fruit body ASI2029


text: National Taiwan University

text: live

text: Generic

text: Mycelium

characteristic: cell type

text: Cinnamomum kanehirai

characteristic: lab-host

text: Taiwanofungus camphoratus

text: Heterokaryon

text: SMV 1

text: Antrodia cinnamomea cDNA Library

text: ZAP Express Vector


text: Tulane University

text: live

characteristic: INSDC status

text: Generic

text: Reproductive

text: Purified mRNA (MicroPolyA-Pure kit, Ambion) was reverse transcribed using the SMART PCR cDNA synthesis system (CLONTECH) and cloned into the TOPO vector (Invitrogen) for colony PCRs and sequencing.

text: Adult

characteristic: dev-stage

text: Drosophila mojavensis

text: Male

text: Male Reproductive Tract cDNA Library


text: Tulane University

text: live

text: Generic

characteristic: NCBI submission model

text: Reproductive

text: Adult

text: Drosophila mojavensis

text: Male

characteristic: sex

text: Male Testis cDNA Library


text: Laboratorio Nazionale CIB

text: live

text: Generic

characteristic: NCBI submission package

text: embryonic week 18 post-fertilization

text: Brain

characteristic: organ

text: Homo sapiens

text: SstI

text: XhoI

text: Right frontal lobe

text: Fetal Brain 18

text: Plasmid

text: pBluecript II (SK-)


text: Laboratorio Nazionale CIB

text: live

text: Generic

text: T lymphoblast; acute lymphoblastic leukemia

characteristic: cell-line

text: Homo sapiens

characteristic: organism

text: SalI

text: NotI

characteristic: title

text: Plasmid


characteristic: External Id

text: Laboratorio Nazionale CIB

text: live

text: Generic

text: embryonic week 15 post-fertilization

text: Brain

text: Homo sapiens

text: SalI

text: NotI

text: Right frontal lobe

characteristic: tissue-type

text: Fetal Brain 15

text: Plasmid


text: Laboratorio Nazionale CIB

characteristic: INSDC center name

text: live

text: Generic

text: week 39

text: Placenta

text: Homo sapiens

text: SalI

text: NotI

text: Placenta

text: Plasmid


text: UAS, GKVK, Bangalore-560065

text: live

text: Generic

characteristic: cultivar

text: Total RNA was isolated from 4-week-old healthy mulberry leaves, and reverse transcribed using oligodT primers, PCR amplified using specific primers (Cer1F 5'GGTTACTTGGATTATGGTCACAC3' Cer1 R TCCCTTACTAGCCATATCTTTGAA3') the amplified product was cloned into T/A cloning vector (pTZ57R/T).

characteristic: description

text: 4 wk old leaves

text: Escherichia coli

text: Leaf

text: Morus alba

text: Hind III

text: Bisexual

text: Leaf tissue

text: cDNA of mulberry

characteristic: vector


text: Maize Research Institute

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: inbred line 81565

text: drought-tolerant inbred line

text: Zea mays

text: Zea mays inbred line 81565


text: Southwest Foundation for Biomedical Research

text: live

characteristic: INSDC status

text: Generic

text: Subtractive hybridization was performed with liver cDNAs from an opossum with elevated low density lipoprotein cholesterol and liver cDNAs from an opossum with normal low density lipoprotein cholesterol using the PCR-Select cDNA subtraction kit (BD Biosciences Clontech, Palo Alto, CA). The subtracted cDNAs were cloned into the pCR4-TOPO vector, and the ligation mixture was transformed into TOP10 cells.

text: adult

text: Monodelphis domestica

text: liver

text: Opossum subtracted liver cDNAs cloned in pCR4-TOPO

text: plasmid


text: Departement de Biochimie, Universite de Montreal

text: live

text: Generic

text: Bigelowiella natans

text: Bigelowiella natans Non-normalized


text: live

text: Generic

text: Subtractive hybridization was performed with liver cDNAs from an opossum with elevated low density lipoprotein cholesterol and liver cDNAs from an opossum with normal low density lipoprotein cholesterol using the PCR-Select cDNA subtraction kit (BD Biosciences Clontech, Palo Alto, CA). The subtracted cDNAs were digested with Rsa I, and cloned into the pcDNA6/V5-His A vector pre-cut with Pme I. The ligation mixture was transformed into OmniMAX-T1 cells.

text: adult

characteristic: dev-stage

text: Monodelphis domestica

text: liver

text: Opossum subtracted liver cDNAs cloned in pcDNA6/V5-His A

text: plasmid

text: pcDNA6/V5-His A


text: Nanjing Agricultural University

text: live

text: Generic

characteristic: NCBI submission model

text: Gossypium hirsutum

text: cotton fiber

text: Gossypium hirsutum 5-25 dpa fiber library


text: live

text: Generic

text: Antonospora locustae

text: Antonospora locustae Non-normalized long fraction


text: Prince of Songkhla University

text: live

text: Generic

text: Total RNA was isolated from haemocyte of Penaeus monodon. The mRNA was further purified, reverse transcribed and cloned to pBK-CMV Phagemid Vector.

text: Haemocyte

text: Penaeus monodon

text: EcoR I

text: Xho I

text: Haemocyte cDNA plasmid library

text: Plasmid

text: pBK-CMV Phagemid Vector


text: Universities of Nottingham and Edinburgh

text: live

text: Generic

text: A survey of genes expressed in the snail Helix aspersa

text: adult

text: foot

text: Cornu aspersum

text: Helix aspersa cDNA library

text: plasmid


text: live

text: Generic

text: A survey of genes expressed in the land snail Mandarina ponderosa

text: adult

text: foot

text: Mandarina ponderosa

text: Mandarina ponderosa cDNA library

text: plasmid


text: Murdoch University

text: live

text: Generic

text: Total RNA was extracted from P. nodorum strain SN15 infected wheat cv. Amery at 14 dpi. Library contains both fungal and plant sequences.

text: Sporulation

text: Triticum aestivum/Phaeosphaeria nodorum mixed EST library

text: cDNA library of sporulating Phaeosphaeria nodorum SN15 on Wheat cv. Amery


text: Murdoch University

text: live

text: Generic

text: Parastagonospora nodorum SN15

text: S. nodorum oleate-induced cDNAs


text: University of Antwerp

text: live

text: Generic

text: adult

text: Daphnia magna

text: female

characteristic: sex

text: whole body

text: Daphnia magna cDNA library


text: The University of Georgia, Department of Plant Biology

text: live

text: Generic

characteristic: NCBI submission package

text: The library was prepared from polyA+ RNA from the roots of 1-year-old loblolly pine (Pinus taeda) cuttings that were rooted and then planted in washed sand. The rooted cuttings were maintained for 139 days (July 28 2003 harvest) under ambient conditions in a local greenhouse. They were kept on a weekly regimen of 0.5x nutrient-complete Hoagland's solution and supplemented with additional water sufficient to maintain a 15% soil moisture content. Starting five weeks prior to harvesting roots for mRNA preparation, the weekly fertilizer regimen for the potted trees was supplemented with 500 uM H3BO3 (5 applications). Double-stranded cDNA was cloned unidirectionally into pSL1180. Inserts can be excised with EcoRI (5' end) and XhoI (3' end).

text: DH10B-T1 phage-resistant E. coli

characteristic: lab-host

text: Root

text: Pinus taeda

text: EcoRI

text: XhoI

text: 3 CCLONES

characteristic: strain

text: Roots plus added boron


text: live

text: Generic

text: The library was prepared from polyA+ RNA from the roots of 1-year-old loblolly pine (Pinus taeda) cuttings that were rooted and then planted in washed sand. The rooted cuttings were maintained for 139 days (July 28 2003 harvest) under ambient conditions in a local greenhouse. They were kept on a weekly regimen of 0.5x nutrient-complete Hoagland's solution and supplemented with additional water sufficient to maintain a 15% soil moisture content. Starting five weeks prior to harvesting roots for mRNA preparation, the weekly fertilizer regimen for the potted trees was modified to omit calcium from the 0.5x Hoagland's solution (5 applications). For two days prior to starting the modified fertilizer regimen, pots were flushed extensively with water to reduce residual levels of calcium. Double-stranded cDNA was cloned unidirectionally into pSL1180. Inserts can be excised with EcoRI (5' end) and XhoI (3' end).

text: DH10B-T1 phage-resistant E. coli

text: Root

characteristic: organ

text: Pinus taeda

characteristic: organism

text: EcoRI

text: XhoI

text: 3 CCLONES

text: Roots minus calcium


characteristic: External Id

text: live

text: Generic

text: The library was prepared from polyA+ RNA from the roots of 1-year-old loblolly pine (Pinus taeda) cuttings that were rooted and then planted in washed sand. The rooted cuttings were maintained for 136 days (July 25 2003 harvest) under ambient conditions in a local greenhouse. They were kept on a weekly regimen of 0.5x nutrient-complete Hoagland's solution and supplemented with additional water sufficient to maintain a 15% soil moisture content. Starting five weeks prior to harvesting roots for mRNA preparation, the weekly fertilizer regimen for the potted trees was modified to omit nitrogen from the 0.5x Hoagland's solution (5 applications). For two days prior to starting the modified fertilizer regimen, pots were flushed extensively with water to reduce residual levels of nitrogen. Double-stranded cDNA was cloned unidirectionally into pSL1180. Inserts can be excised with EcoRI (5' end) and XhoI (3' end).

text: DH10B-T1 phage-resistant E. coli

text: Root

text: Pinus taeda

text: EcoRI

text: XhoI

text: 3 CCLONES

text: Roots minus nitrogen

characteristic: title


text: Cold Spring Harbor Laboratory

characteristic: INSDC center name

text: live

text: Generic

text: Stratagene ZAP Express cDNA Synthesis Kit. The library was size-fractionated to enrich for large inserts.

text: Ovules

text: Cycas rumphii

text: XhoI

text: Eco RI

text: Female

text: Cycas ovule (NYBG)

text: Plasmid

text: pBK-CMV


text: Instituto de Quimica, Universidade de Sao Paulo

text: live

text: Generic

text: Nose

text: Mus musculus

text: Olfactory epithelium

characteristic: tissue-type

text: Olfactory epithelium - RLM-RACE 5' ESTs


text: China Agricultural University

text: live

text: Generic

text: adult

text: Sus scrofa

text: hermaphrodite

text: XL1-Blue MRF1

text: adipose

text: Normalized porcine adipose tissues cDNA library


text: University of California

text: live

text: Generic

characteristic: cultivar

text: Seeds were germinated in pots (Ehlers) and grown in controlled glass house conditions under natural photoperiod and day/night temperatures of 35/18oC. The growing axillary buds from 10 day old seedlings were harvested from one set of pots, flash frozen and stored at -80oC. Another set of plants was allowed to grow under drought stress induced by withholding water. The growing axillary buds were harvested from 4-day drought stressed seedlings, then flash frozen (Sudhakar). The frozen samples were used for RNA extraction by a TRIzol method (Sudhakar, Mandal, Fenton). A total of 500 microgram each from three stressed and three unstressed sub-samples were pooled to total 1000 microgram of total RNA. mRNA was isolated using PolyATtrack (Promega). Five micrograms of poly(A) mRNA was used for cDNA synthesis (Stratagene). A primary cDNA library was produced using a lambda ZAP XR cDNA Synthesis Kit(Stratagene). One million pfu from the primary library were mass excised to produce a phagemid population (Sudhakar). Phagemids were plated, plasmid DNA purified, cDNA clones archived, and DNA sequences determined bi-directionally using an ABI3730 at the Genomics Core Instrumentation facility, University of California, Riverside (Choi, Kingan). Sequences were processed (Wanamaker) using the HarvEST pipeline (http://harvest.ucr.edu) to remove vector and cloning oligo sequences and various contaminants, and to trim to a high quality region. Sequences that retained a phred 17 region of at least 100 bases were deposited to GenBank.

characteristic: description

text: Seedling

text: E. coli TJC121

text: Vigna unguiculata

text: EcoRI

text: XhoI

text: growing axillary bud

text: Cowpea CB27 Axillary Bud cDNA Library - UCRVU02

text: Phagemid pBluescript SK(-)

text: Lambda Uni-ZAP XR, excised phagemid


characteristic: INSDC first public

text: live

text: Generic

text: The library was prepared from polyA+ RNA from the roots of 1-year-old loblolly pine (Pinus taeda) cuttings that were rooted and then planted in washed sand. The rooted cuttings were maintained for 50 days (May 1 2003 harvest) under ambient conditions in a local greenhouse. They were kept on a weekly regimen of 0.5x nutrient-complete Hoagland's solution and supplemented with additional water sufficient to maintain a 15% soil moisture content. Twenty-four hours (24h) prior to harvesting roots for mRNA preparation, the potted trees were placed in a dark growth chamber at 25 C. Double-stranded cDNA was cloned unidirectionally into pSL1180. Inserts can be excised with EcoRI (5' end) and XhoI (3' end).

text: DH10B-T1 phage-resistant E. coli

text: Root

text: Pinus taeda

text: EcoRI

text: XhoI

text: 3 CCLONES

text: Roots, dark

characteristic: vector


text: Cold Spring Harbor Laboratory

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: leaf

text: Ginkgo biloba

text: XhoI

text: Eco RI

text: male

text: Ginkgo male leaf (NYBG)

text: Plasmid

text: pBK-CMV


text: National University of Singapore

text: live

text: Generic

characteristic: NCBI submission model

text: Library was prepared by Sheik Fadil Ryan Ramjan, National University of Singapore, Singapore.

text: Acarus siro

text: Acarus siro cDNA library


text: live

text: Generic

text: The library was prepared from polyA+ RNA from the roots of 1-year-old loblolly pine (Pinus taeda) cuttings that were rooted and then planted in washed sand. The rooted cuttings were maintained for 143 days (August 1 2003 harvest) under ambient conditions in a local greenhouse. They were kept on a weekly regimen of 0.5x nutrient-complete Hoagland's solution and supplemented with additional water sufficient to maintain a 15% soil moisture content. Twenty-four hours (24h) prior to harvesting roots for mRNA preparation, the potted trees were watered with 500 uM FeSO4 (aq) until the sand was saturated. Double-stranded cDNA was cloned unidirectionally into pSL1180. Inserts can be excised with EcoRI (5' end) and XhoI (3' end).

text: DH10B-T1 phage-resistant E. coli

text: Root

text: Pinus taeda

text: EcoRI

text: XhoI

text: 3 CCLONES

text: Roots plus added iron


text: University of California, Riverside

text: live

text: Generic

text: Epithelial

characteristic: cell type

text: The cDNA was cloned into the SalI/NotI sites of pSPORT1.

text: Adult

characteristic: dev-stage

text: Aedes aegypti

text: SalI

text: NotI

text: Female

characteristic: sex

text: Midgut & Malpighian tubules

text: Aedes aegypti midgut&MT pSPORT Library


text: Danish Institute of Agricultural Sciences

text: live

text: Generic

characteristic: NCBI submission package

text: Normalised cDNA library, adult muscle (longissimus dorsi). Single pass sequencing.

text: Sus scrofa

text: EcoRI

text: NotI

text: muscle


text: live

text: Generic

text: Normalised cDNA library, adult placenta. Single pass sequencing.

text: Sus scrofa

text: EcoRI

text: NotI

text: placenta


text: Dept. Life Sciences

text: live

text: Generic

text: Crantz

text: tuber

characteristic: organ

text: Manihot esculenta

text: EcoRI

text: XhoI

text: cassava tuber

text: Phage

text: pBluescript II SK


text: University of Hyogo

text: live

text: Generic

text: Adiantum capillus-veneris

characteristic: organism

text: prothallium

text: Adiantum capillus-veneris prothallium


characteristic: External Id

text: live

text: Generic

text: The library was prepared from polyA+ RNA from the roots of 1-year-old loblolly pine (Pinus taeda) cuttings that were rooted and then planted in washed sand. The rooted cuttings were maintained for 154 days (August 12 2003 harvest) under ambient conditions in a local greenhouse. They were kept on a weekly regimen of 0.5x nutrient-complete Hoagland's solution and supplemented with additional water sufficient to maintain a 15% soil moisture content. Twenty-four hours (24h) prior to harvesting roots for mRNA preparation, the potted trees were watered with 5 mM AlCl3 (aq) until the sand was saturated. Double-stranded cDNA was cloned unidirectionally into pSL1180. Inserts can be excised with EcoRI (5' end) and XhoI (3' end).

text: DH10B-T1 phage-resistant E. coli

characteristic: lab-host

text: Root

text: Pinus taeda

text: EcoRI

text: XhoI

text: 3 CCLONES

characteristic: strain

text: Roots plus added aluminum


text: Beijing Acadamy of Agricultural and Forestry Sciences

characteristic: INSDC center name

text: live

text: Generic

text: Triticum aestivum

text: Spikelet

characteristic: tissue-type

text: DDRT-RCHW

characteristic: title


text: The Wellcome Trust Sanger Institute

characteristic: INSDC first public

text: live

text: Generic

text: country: Turkey:Ankara

characteristic: description

text: piroplasm

text: Ankara (clone D7)

characteristic: isolate

text: Bos taurus (cow)

text: Theileria annulata

text: Theileria annulata piroplasm


text: The Wellcome Trust Sanger Institute

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: country: Turkey:Ankara

text: merozoite

text: Ankara (clone D7)

text: Bos taurus (cow)

text: Theileria annulata

text: Theileria annulata merozoite


text: The Wellcome Trust Sanger Institute

text: live

text: Generic

characteristic: NCBI submission model

text: country: Turkey:Ankara

text: macroschizont

text: Ankara (clone D7)

text: Bos taurus (cow)

text: Theileria annulata

text: Theileria annulata macroschizont


text: Gsf

characteristic: INSDC secondary accession

text: live

text: Generic

characteristic: SRA accession

text: ozone induced cDNA library

text: Fagus sylvatica

text: leaf

text: Fagus sylvatica leaf


text: National Institute of Genetics

text: live

text: Generic

characteristic: NCBI submission package

text: Protonemata were inoculated on BCDATG medium for every ca. 5 days. Protoplasts were isolated from the protonemata, further incubated at 25C under continuous light for 2-3 days. The regenerated cells, which were rich in cells at a stage during the first asymmetric cell division, were collected. Total RNA was extracted for constructing a full-length cDNA library. synonym: Physcomitrella patens subsp. patens

text: at the first asymmetric cell division of protoplasts

characteristic: dev-stage

text: Physcomitrium patens

text: regenerated protoplasts (chloronemata)

text: pphf full-length cDNA libary


text: The University of Georgia, Department of Plant Biology

text: live

text: Generic

text: The library was prepared from polyA+ RNA isolated from stems of loblolly pine (Pinus taeda) hedges challenged with the necrotrophic fungus Fusarium circinatum, the inciting agent of pitch canker disease. Four pitch canker resistant hedges were inoculated in November, 2003. For each hedge, multiple succulent shoot tips were inoculated with 1 mL of a 500 spores/mL solution after excision. The terminal 1 cm of each challenged stem was harvested for mRNA isolation 10 days after challenge and bulked.

text: DH10B-T1 phage-resistant E. coli

text: Shoot tip

characteristic: organ

text: Pinus taeda

characteristic: organism

text: EcoRI

text: XhoI

text: 3 CCLONES

text: Stem Response Resistant

characteristic: vector


characteristic: External Id

text: Temple University, School of Medicine

text: live

text: Generic

text: Macaca mulatta

text: Macaca mulatta (Latham KE)


text: USDA - ARS

characteristic: INSDC center name

text: live

text: Generic

text: A high quality EST with at least 100 contiguous bases at Trace Tuner score of 20 or better. Larva reared on artifical diet by J.M. Tropp (USDA-ARS-BIIR); cDNA library construction and sequencing by L.E. Hunnicutt (USDA-ARS-USHRL).

text: Larva, 5th-instar

characteristic: lab-host

text: Anoplophora glabripennis

text: EcoRI

text: XhoI

text: Whole body

text: 5th-instar Anoplophora glabripennis larva

text: pBluescript II SK+


text: live

text: Generic

text: Samsun

characteristic: cultivar

text: Nicotiana tabacum cv. Samsun, induced by LPS.

text: Nicotiana tabacum

text: Cultured tobacco cells

characteristic: title


characteristic: INSDC first public

text: live

text: Generic

text: The library was prepared from polyA+ RNA from the roots of 1-year-old loblolly pine (Pinus taeda) cuttings that were rooted and then planted in washed sand. The rooted cuttings were maintained for 136 days (July 25 2003 harvest) under ambient conditions in a local greenhouse. They were kept on a weekly regimen of 0.5x nutrient-complete Hoagland's solution and supplemented with additional water sufficient to maintain a 15% soil moisture content. Starting five weeks prior to harvesting roots for mRNA preparation, the weekly fertilizer regimen for the potted trees was modified to omit sulfur from the 0.5x Hoagland's solution (5 applications). For two days prior to starting the modified fertilizer regimen, pots were flushed extensively with water to reduce residual levels of sulfur. Double-stranded cDNA was cloned unidirectionally into pSL1180. Inserts can be excised with EcoRI (5' end) and XhoI (3' end).

characteristic: description

text: DH10B-T1 phage-resistant E. coli

text: Root

text: Pinus taeda

text: EcoRI

text: XhoI

text: 3 CCLONES

characteristic: strain

text: Roots minus sulfur