text: Royal Botanic Gardens, Kew

text: live

text: Generic

characteristic: NCBI submission package

text: Clontech PCR-Select cDNA Subtraction Kit

characteristic: dev-stage

text: Escherichia coli JM109

text: apical bud

characteristic: organ

text: Castanea sativa

text: ApaI, AatII, SphI, Bst ZI, NcoI,

text: SpeI, EcoRI, NotI, Bst ZI, PstI,

text: Castanea sativa bud dormancy

text: plasmid

text: pGEM-T Easy


status: PUBLIC

characteristic: External Id

text: Royal Botanic Gardens, Kew

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: description

tag: attribute

text: Escherichia coli JM109

characteristic: lab-host

text: apical bud

text: Castanea sativa

characteristic: organism

text: ApaI, AatII, SphI, Bst ZI, NcoI,

text: SpeI, EcoRI, NotI, Bst ZI, PstI,

text: Castanea sativa bud non-dormancy

characteristic: title

text: plasmid

text: pGEM-T Easy

characteristic: vector


text: Institute of Nuclear and Biological Technologies,Xinjiang Academy of Agricultural Sciences

text: live

text: Generic

text: Xinchun 6

characteristic: cultivar

text: cDNA-AFLP

text: Escherichia coli ATCC 47093

text: Triticum aestivum

text: Leaf

characteristic: tissue-type

text: Wheat drought Library


text: College of Life Science, Sichuan University

text: live

text: Generic

text: green

characteristic: cell type

text: Cells were grown in defined medium containing 1.5M NaCl, light for 16 hours and dark for 8 hours at 25degC, collected by centrifugation at 4degC when grown to a density of 6x10E5.

text: Dunaliella salina

text: Dunaliella salina subtracted normalized cDNA library

text: Plasmid

text: pDNR-LIB


text: Faculty of Sciences of Lisbon University

text: live

text: Generic

characteristic: NCBI submission package

text: The cDNA library was constructed using CloneMiner TM cDNA library Construction Kit, with Gateway technology. cDNA sequences were cloned into pDONRTM222 Gateway vector.

text: nonpollinated

text: E.coli

text: flower

text: Ophrys fusca

text: labellum

text: Ophrys fusca nonpollinated cDNA library

text: plasmid


text: live

text: Generic

text: 2 days and 4 days after pollination

characteristic: dev-stage

text: E.coli

text: flower

text: Ophrys fusca

text: labellum

text: Ophrys fusca pollinated cDNA library

text: plasmid


text: Academia Sinica

text: live

text: Generic

text: fry

text: head kidney

characteristic: organ

text: Epinephelus coioides

text: hermaphrodite

characteristic: sex

text: GIV-treated Orange-spotted grouper fry Library


characteristic: External Id

text: School of Agriculture and Biology, Shanghai Jiaotong University

characteristic: INSDC center name

text: live

text: Generic

text: 2 & 3-day Arabidopsis thaliana seedlings were immersed in loading solution (MS liquid medium+2 M glycerol+0.4 M sucrose) for 20 minutes at room temperature. Loading solution was removed from the cryovial and rapidly replaced by filtered sterilized cryoprotective solution PVS2 (30% w/v glycerol, 15% w/v ethylene glycol and 15% w/v DMSO in liquid MS medium supplemented with 0.4 M sucrose) and left at 0degC for 50 minutes. cDNA library was derived from these seedlings. cDNA synthesis was initiated using a oligo(dT) primer. Double-stranded cDNA was blunted, digested with EcoRI and MseI restriction endonuclease, ligated to EcoRI and MseI adaptors, using cDNA-AFLP to screen the different expression gene, and cloned into the pMD18-T simple vector.

text: 2d and 3d

text: E. coli DH5-alpha

text: Arabidopsis thaliana

text: EcoRI

characteristic: strain

text: Seedlings

text: Arabidopsis vitrification solution treatment library

text: Plasmid

text: pMD18-T simple vector


characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Sequenced Pto and Fen genes from various tomato accessions

text: Solanum lycopersicum

text: Tomato Genome, Pto and Fen variation Search Library


text: Virginia State University

text: live

text: Generic

characteristic: NCBI submission model

text: Resistant to SGG strain of Colletotrichum gleosporoides; genotype TDa 87-01091

characteristic: description

text: Dioscorea alata

text: leaf


text: National Research Institute of Aquaculture

text: live

text: Generic

text: adult

text: Pseudocentrotus depressus

characteristic: organism

text: female

text: ovary

text: Normalized directional Pseudocentrotus depressus cDNA library

characteristic: title


text: Sun Yat-sen University

text: live

text: Generic

text: Sonneratia caseolaris

text: whole leaf

text: cDNA library of Sonneratia caseolaris leaves


text: Instituto Potosino de Investigacion Cientifica y Tecnologica

text: live

text: Generic

text: creole landrace Pv-2094

characteristic: cultivar

text: cDNA library was constructed using the PCR-Select cDNA Subtraction Kit (Clontech, Palo Alto, CA). RNA isolated from root tissues inoculated 8 and 16 h with R. solani were used as source for the tester sample. The driver was derived from RNA of non-inoculated root tissues harvested at the same time points, and was used to remove sequences from genes expressed under both conditions.

text: Seedling

text: E. coli

characteristic: lab-host

text: Phaseolus vulgaris

text: EcoRI

text: EcoRI

text: roots

text: Subtractive cDNA library of bean roots inoculated 8 and 16 h with Rhizoctonia solani

text: Plasmid

characteristic: vector


text: University of Sydney

text: live

text: Generic

characteristic: NCBI submission package

text: Chromera velia isolated from stony coral Leptastrea purpurea from One Tree Island, Australia. Cells were grown in f/2 medium and salinity 40 g.L-1; incubated at 25 C under 12:12 hours light:dark cycle with light intensity of 165 umol.m-2.s-1; 3-4 weeks old. Cell pellets were snap frozen in liquid N2, ground in liquid N2 until a fine powder and total RNA isolated using Ribozol RNA reagent.

text: Chromera velia

text: EcoRI

text: XhoI

text: Chromera velia CvLp_vc08/1 cDNA library

text: phagemid

text: pBluescript SK-


text: Agricultural Research Council (CRA)

text: live

text: Generic

text: Expressed tags library obtained by cDNA-AFLP analysis of Cucumis melo stem samples during interaction with different races of Fusarium oxysporum f. sp. melonis

text: Stem

text: Cucumis melo

text: C. melo-F. oxysporum f.sp. melonis interact lib.


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT)-primed cDNA and cloned into vector pDNR-LIB (Clontech Laboratories, Inc., Mountain View, CA) using the SMART system (BD Biosciences, San Jose, CA). To generate the first strand, polyA RNA was primed with an oligo(dT) primer (5'-ATTCTAGAGGCCGAGGCGGCCGACATG(T)30 VN-3', where V = A, G, or C). The second strand was synthesized by PCR using primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' followed by digestion of the SfiI sites. cDNA was size selected then ligated into pDNR-LIB. Library is normalized and is size-selected for inserts <1 kb. Library was created and sequencing done by the DOE Joint Genome Institute (Walnut Creek, CA).

text: Botryococcus braunii

text: SfiIA

text: SfiIB

text: UTEX 572

text: CFNW Botryococcus braunii UTEX 572 normalized (L)

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT)-primed cDNA and cloned into vector pDNR-LIB (Clontech Laboratories, Inc., Mountain View, CA) using the SMART system (BD Biosciences, San Jose, CA). To generate the first strand, polyA RNA was primed with an oligo(dT) primer (5'-ATTCTAGAGGCCGAGGCGGCCGACATG(T)30 VN-3', where V = A, G, or C). The second strand was synthesized by PCR using primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' followed by digestion of the SfiI sites. cDNA was size selected then ligated into pDNR-LIB. Library is normalized and is size-selected for inserts >1 kb. Library was created and sequencing done by the DOE Joint Genome Institute (Walnut Creek, CA).

text: Botryococcus braunii

text: SfiIA

text: SfiIB

text: UTEX 572

text: CFNX Botryococcus braunii UTEX 572 normalized (H)

text: pDNR-LIB


text: Ohio Wesleyan University

text: live

text: Generic

text: Dissected maternal follicle from mixed staged embryos

text: Poeciliopsis turneri

text: Female

text: Maternal follicle

characteristic: tissue-type

text: Poeciliopsis turneri maternal follicle cDNA library


text: Ohio Wesleyan University

text: live

text: Generic

text: Poeciliopsis presidionis

text: Female

text: Maternal follicle

text: Poeciliopsis presidionis maternal follicle cDNA library


text: Agriculture and Agri-Food Canada

text: live

text: Generic

text: This is a meta-genomic cDNA library created by infection of A. candida 2VRR onto susceptible Brassica juncea

text: Brassica juncea/Albugo candida mixed genomic library

text: Infected plant material

text: B. juncea infected with A. candida 2VRR


text: Agriculture and Agri-Food Canada

text: live

text: Generic

text: Sporangiospore EST data for A. candida 2VRR

text: Albugo candida

text: sporangiospores

text: A. candida 2VRR spore ESTs


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT)-primed cDNA and cloned into vector pDNR-LIB (Clontech Laboratories, Inc., Mountain View, CA) using the SMART system (BD Biosciences, San Jose, CA). To generate the first strand, polyA RNA was primed with an oligo(dT) primer (5'-ATTCTAGAGGCCGAGGCGGCCGACATG(T)30 VN-3', where V = A, G, or C). The second strand was synthesized by PCR using primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' followed by digestion of the SfiI sites. cDNA was size selected from 2-8kb using 1.1% agarose gel electrophoresis, then ligated into pDNR-LIB. Normalized library was created and sequencing performed by the DOE Joint Genome Institute (Walnut Creek, CA).

text: Euonymus alatus

text: SfiIA

text: SfiIB

text: CCXA Euonymus alatus seed tissues collected from 8/14 to 9/19/2007 normalized

text: pDNR-LIB


text: University Santiago de Compostela

text: live

text: Generic

text: Total RNA was extracted from pooled tissues of Scophthalmus maximus. cDNA library was constructed with equal amounts of RNA from each tissue using the Uni-ZAP XR Vector. The cDNA library was generated as part of Scophthalmus maximus EST project.

text: liver, spleen head kidney, pyloric caeca and thymus

characteristic: organ

text: Scophthalmus maximus

text: EcoRI

text: XhoI

text: Immune

text: S. maximus Enteromyxum cDNA library

text: Uni-ZAP XR vector


text: University Santiago de Compostela

text: live

text: Generic

text: Scophthalmus maximus

text: EcoRI

text: XhoI

text: Immune

text: S. maximus control cDNA library (2010)

text: Uni-ZAP XR vector


text: Swedish University of Agricultural Sciences

text: live

text: Generic

text: Picea abies

text: Hermaphrodite

characteristic: sex

text: Heterobasidion parviporum inoculated spruce bark

text: Picea abies cDNA-AFLP full-sib


characteristic: External Id

text: National Institute of Agrobiological Sciences

text: live

text: Generic

text: Ostrinia nubilalis

text: pupae whole body cDNA


characteristic: INSDC center name

text: live

text: Generic

text: Ostrinia nubilalis

text: Pharate pupal fat body cDNA


text: live

text: Generic

text: Ostrinia nubilalis

text: male

text: Adult antenna cDNA


text: live

text: Generic

text: Ostrinia nubilalis

text: male

text: Adult hair pencil cDNA


text: live

text: Generic

text: Ostrinia nubilalis

text: wandering larvae epidermis cDNA


text: live

text: Generic

text: Ostrinia nubilalis

text: pharate pupal whole body cDNA


text: Crop Improvement Division, National Research Centre for Banana (ICAR)

text: live

text: Generic

text: Manoranjitham (AAA)

text: Four months old tissue cultured Musa accuminata cultivars Manoranjitham (AAA) and Robusta (AAA) were challenged with spores of Mycospharella eumusae, cDNA was synthesized from the pooled polyA+ RNA obtained from leaf tissues collected at every 6hours up to 72hrs post infection. Suppressive subtractive hybridization was performed with the two pools of cDNA obtained from the cultivars namely cv. Manoranjitham as tester and cv. Robusta as driver. The subtracted cDNA fragments were cloned into TA cloning vector (pGEMT-Easy) and sequenced.

text: 4 months old tissue cultured plants

characteristic: dev-stage

text: Musa acuminata AAA Group

text: Eco RI

text: Eco RI

text: Hermaphordite

text: Leaf

text: Musa acuminata cv. Manoranjitham Leaf Tissue Specific Library

text: PLASMID

text: pGEMT-Easy


text: The J. Craig Venter Institute

characteristic: INSDC first public

text: live

text: Generic

text: A cDNA library was constructed using cultured infected erythrocytes (Florida strain) using the Creator SMART cDNA library construction kit (Clontech). cDNA in the size range of 0.3 -2kb was normalized using the Trimmer normalization kit (Evrogen).

text: Theileria equi

text: JCVI-BE


text: Zhejiang University

characteristic: INSDC last update

text: live

text: Generic

text: A suppression subtractive library is constructed using B.bassiana wild-type mRNA as tester and the B.bassiana mRNA under the thermal stress as driver. The targeted genes were screened and cloned into pMD18-T vector to sequence.

text: Escherichia coli DH5alpha

text: Beauveria bassiana

characteristic: strain

text: SSH library enriched by heat stress-responsive genes


text: College of Animal Sciences, Zhejiang University

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Haliotis diversicolor supertexta

text: cDNA Library of gastropod abalone hemocytes


text: Embrapa Recursos Geneticos e Biotecnologia

text: live

text: Generic

text: As part of genolyptus project, seed-derived E. grandis plants were grown in vitro for 2 months in 1/4 Murashige and Skoog medium (MS, Invitrogen) solidified by 0.7% (w/v) Phytoagar (Duchefa) at 26degC and a photoperiod of 16 h . Total RNA was extracted from 100-200 mg of tissues previously pulverized in mortars with pestles and liquid nitrogen using the PureLink Plant RNA Reagent Kit (Invitrogen) according to the multi-miniprep protocol instructions of the manufacturer. Polyadenylated RNA was subsequently purified from total RNA using the Oligotex mRNA Mini kit (Qiagen). RNA purity and quality were checked by both agarose gel electrophoresis and spectrophotometric measurements at 260 and 280 nm. Complementary DNA libraries were constructed using 1-5 ug mRNA and kits of the Superscript Plasmid System with Gateway Technology for cDNA Synthesis and Cloning (Invitrogen) and instructions of the manufacturer. Eluted fractions of double-stranded cDNA were stored at -20 degC and further ligated into pSport1 (Invitrogen). Ligated products were transformed into Escherichia coli Electromax DH10B competent cells (Invitrogen) using a Gene Pulser ll Electroporation System (Bio-Rad).

characteristic: description

text: 2-Month-old, in vitro grown seedlings

text: Escherichia coli DH10B

text: Whole seedling (leaf, stem and root)

text: Eucalyptus grandis

characteristic: organism

text: SaII

text: NotI

text: Seedlings (EUGR-SE)

text: plasmid


text: live

text: Generic

text: As part of genolyptus project, Seed-derived E. grandis plants were grown in vitro for 2 months in 1/4 Murashige and Skoog medium (MS, Invitrogen) solidified by 0.7% (w/v) Phytoagar (Duchefa) at 26 degC and a photoperiod of 16 h . Groups of 3-4 plantlets were separately sprayed three times with 10 mg/L 2,4- dichlorophenoxyacetic acid, 10 mg/L kinetin, 10 mg/L ethephon, 10 mg/L abscisic acid, 10 mg/L gibberellic acid, 1 g/L paclobutrazol, 1 mM salicylic acid, 1 mM methyl jasmonic acid, 1 mM paraquat or water as control. Groups of 3-4 plantlets were sprayed three-times with water and then irradiated with ultraviolet light (354 nm, 40 cm apart) for 4 h or for two periods of 4 h each. Another group of 3-4 plants were water sprayed and kept at 10 degC during 16 h. After the series of treatments, all plants were maintained under normal growth conditions during 24 or 48 h before freezing them in liquid nitrogen. One group of (control) plants was frozen in liquid nitrogen without any treatment. Total RNA was extracted from 100-200 mg of tissues previously pulverized in mortars with pestles and liquid nitrogen using the PureLink Plant RNA Reagent Kit (Invitrogen) according to the multi-miniprep protocol instructions of the manufacturer. Polyadenylated RNA was subsequently purified from total RNA using the Oligotex mRNA Mini kit (Qiagen). RNA purity and quality were checked by both agarose gel electrophoresis and spectrophotometric measurements at 260 and 280 nm. Complementary DNA libraries were constructed using 1-5 ug mRNA and kits of the Superscript Plasmid System with Gateway Technology for cDNA Synthesis and Cloning (Invitrogen) and instructions of the manufacturer. Eluted fractions of double-stranded cDNA were stored at -20 degC and further ligated into pSport1 (Invitrogen). Ligated products were transformed into Escherichia coli Electromax DH10B competent cells (Invitrogen) using a Gene Pulser ll Electroporation System (Bio-Rad).

text: 2-Month-old, in vitro grown seedlings

text: Escherichia coli DH10B

text: Whole seedling (leaf, stem and root)

text: Eucalyptus grandis

text: SaII

text: NotI

text: Treated seedlings (EUGR-TS)

characteristic: title

text: plasmid


text: live

text: Generic

text: As part of genolyptus project, differentiating xylem was collected by scraping the exposed vascular tissue after the removal of the 0.5-1 cm-tick stem bark from 4-year-old E. grandis trees located in commercial plantations of Veracel Celulose S.A. (Eunapolis, BA, Brazil). Total RNA was extracted from 100-200 mg of tissues previously pulverized in mortars with pestles and liquid nitrogen using the PureLink Plant RNA Reagent Kit (Invitrogen) according to the multi-miniprep protocol instructions of the manufacturer. Polyadenylated RNA was subsequently purified from total RNA using the Oligotex mRNA Mini kit (Qiagen). RNA purity and quality were checked by both agarose gel electrophoresis and spectrophotometric measurements at 260 and 280 nm. Complementary DNA libraries were constructed using 1-5 ug mRNA and kits of the Superscript Plasmid System with Gateway Technology for cDNA Synthesis and Cloning (Invitrogen) and instructions of the manufacturer. Eluted fractions of double-stranded cDNA were stored at -20 degC and further ligated into pSport1 (Invitrogen). Ligated products were transformed into Escherichia coli Electromax DH10B competent cells (Invitrogen) using a Gene Pulser ll Electroporation System (Bio-Rad).

text: Differentiating xylem

text: Escherichia coli DH10B

characteristic: lab-host

text: Stem

text: Eucalyptus grandis

text: SaII

text: NotI

text: Xylem (EUGR-XY)

text: plasmid


text: live

text: Generic

text: As part of genolyptus project, young leaves infected by Puccinia psidii were collected from 6-month-old, field-grown E. grandis at experimental orchards of Aracruz Celulose S.A. (Barra do Ribeiro, RS, Brazil). Total RNA was extracted from 100-200 mg of tissues previously pulverized in mortars with pestles and liquid nitrogen using the PureLink Plant RNA Reagent Kit (Invitrogen) according to the multi-miniprep protocol instructions of the manufacturer. Polyadenylated RNA was subsequently purified from total RNA using the Oligotex mRNA Mini kit (Qiagen). RNA purity and quality were checked by both agarose gel electrophoresis and spectrophotometric measurements at 260 and 280 nm. Complementary DNA libraries were constructed using 1-5 ug mRNA and kits of the Superscript Plasmid System with Gateway Technology for cDNA Synthesis and Cloning (Invitrogen) and instructions of the manufacturer. Eluted fractions of double-stranded cDNA were stored at -20 degC and further ligated into pSport1 (Invitrogen). Ligated products were transformed into Escherichia coli Electromax DH10B competent cells (Invitrogen) using a Gene Pulser ll Electroporation System (Bio-Rad).

text: Young (< 4-cm long) leaves infected by Puccinia psidii

text: Escherichia coli DH10B

text: Leaf

text: Eucalyptus grandis

text: SaII

text: NotI

text: Young leaves infected by Puccinia psidii (EUGR-PU)

text: plasmid

characteristic: vector


text: Max Planck Institute for Molecular Genetics

text: live

text: Generic

characteristic: NCBI submission package

text: KWS2320 (double haploid, monogerm breeding line)

characteristic: cultivar

text: Beta vulgaris subsp. vulgaris

text: pooled tissues (sugar beet leaf, developing root, storage root, inflorescence)

text: sugar beet pooled tissues cDNA library


text: Centro de Investigacion y Estudios Avanzados del IPN

text: live

text: Generic

text: Cavendish ?Grand Nain?

text: E.coli DH5a

text: fruit

characteristic: organ

text: Musa acuminata AAA Group

text: M13 forward

text: M13 reverse

text: pulp

characteristic: tissue-type

text: Banana fruit differential display

text: plasmid

text: pCR2.1 TOPO TA


characteristic: External Id

text: Georgia Southern University

text: live

text: Generic

text: Zap Express cDNA Synthesis Kit, pBluescript phagemid

text: Tigriopus californicus

text: San Diego Population (32deg45'N,117deg15'W)

text: Whole organism

text: TcalSD


text: BOKU University

characteristic: INSDC center name

text: live

text: Generic

text: mycelia cells

characteristic: cell type

text: obtained from 3´-RACE; from Vietnamese soil contaminated with dioxins

text: stationary phase

text: E.coli

text: Chaetomium sp. INBI 2-26(-)

text: Restriction enzyme at site1 of v

text: Restriction enzyme at site2 of v

text: INBI 2-26(-)

text: mycelium

text: Chaetomium INBI 2-26(-) library.

text: Plasmid

text: pAL-TA


text: Kangwon National University

characteristic: INSDC first public

text: live

text: Generic

text: Panax ginseng

text: Panax ginseng in vitro-cultured adventitious root cDNA library


text: College of Life Science, Wuhan University

characteristic: INSDC last update

text: live

text: Generic

text: apical cell of two-celled proembryo

text: Two-celled proembryo were manually collected from ovules. An LMD (Leica, Bonn, Germany) was used to destroy basal cell of two-celled proembryos to obtain the apical cell. RNA was isolated and cDNA library was constructed by using the Clontech SMART cDNA library construction kit. The cDNAs were directionally ligated into pTripEX2.

text: after first zygote division

characteristic: dev-stage

text: Nicotiana tabacum

text: Sfi IA

text: Sfi IB

text: Tobacco cultivar SR1 apical cell cDNA library

text: Phage


text: live

characteristic: INSDC status

text: Generic

text: basal cell of two-celled proembryo

text: Two-celled proembryo were manually collected from ovules. An LMD (Leica, Bonn, Germany) was used to destroy apical cell of two-celled proembryos to obtain the basal cell. RNA was isolated and cDNA library was constructed by using the Clontech SMART cDNA library construction kit. The cDNAs were directionally ligated into pTripEX2.

text: after first zygote division

text: Nicotiana tabacum

text: Sfi IA

text: Sfi IB

text: Tobacco cultivar SR1 basal cell cDNA library

text: Phage


text: MPI for Molecular Genetics

text: live

text: Generic

characteristic: NCBI submission model

text: adult

text: Balanoglossus clavigerus

text: gonads


text: MPI for Molecular Genetics

text: live

text: Generic

text: Rhabdopleura compacta

text: adult

characteristic: sex

text: complete zooids


text: Institut de Recherche sur la Biologie de l'Insecte

text: live

text: Generic

text: adult

text: Spodoptera littoralis

text: Chelonus inanitus

characteristic: organism

text: female

text: venom glands

text: Chelonus inanitus venom glands adult


text: Huazhong Agricultural University

text: live

text: Generic

text: Valencia

text: The cDNA library was constructed following the Clontech PCR-Select cDNA subtraction Kit (Clontech, USA)

characteristic: description

text: non-embryogenic calli cultured three months in solid medium containing 2, 4-D; embryogenic calli cultured for 45 d in MT liquid medium; embryogenic calli cu

text: Citrus sinensis

text: Hermaphrodite

text: callus

text: Valencia sweet orange callus library

characteristic: title


text: International Center for Tropical Agriculture, CIAT. http://www.ciat.cgiar.org

text: live

text: Generic

text: Total RNA was prepared from common bean leaves, poly(A) RNA was purified and reverse transcribed, and cDNAs were cloned into the pCMVSport6.0 vector (Life technologies) at the International Center for Tropical Agriculture (CIAT), Cali, COLOMBIA. The library was transformed into E. coli EMDH12S cells which were plated on Q-plates with Carbenicillin (100 mg/L). A Q-Bot was used to pick and array colonies into plates and filters.

text: E. coli EMDH12S

characteristic: lab-host

text: Phaseolus vulgaris

text: Not I

text: Sal I

text: Leaf

text: Phaseolus vulgaris leaf EST library CIAT

text: Plasmid

characteristic: vector


text: College of Marine Life Sciences, Ocean University of China

text: live

text: Generic

characteristic: NCBI submission package

text: Zostera marina

text: Zostera marina cDNA library of leaf treated by salt stress


text: College of Veterinary Medicine, Seoul National University

text: live

text: Generic

text: Smooth muscle

text: Omentum was inflamed with gauze and collected for RNA extraction. Complementary DNA (cDNA) was prepared and thereby DD PCR was done.

text: Abdomen

characteristic: organ

text: Canis lupus familiaris

text: Male and Female

text: Beagle

characteristic: strain

text: Omentum

text: Canine Peritonitis Library


characteristic: External Id

text: Project Directorate on Foot-and-Mouth disease

text: live

text: Generic

text: PBMC

text: JM109 (Escherichia coli)

text: Bos indicus x Bos taurus

text: Holstein Friesian x Local crossbred

text: Blood

characteristic: tissue-type

text: Cattle PBMC library

text: Plasmid

text: pGEMT-Easy


text: Universite Politecnica delle Marche

characteristic: INSDC center name

text: live

text: Generic

text: Sparus aurata

text: Liver

text: Sparus aurata liver library


text: Kansas State University

characteristic: INSDC first public

text: live

text: Generic

text: Total RNA was isolated using the Micro RNA isolation kit from Stategene. cDNA library was formed with the SMART kit from Clontech, using RNA from 250 dissected salivary glands. Clones were chosen randomly for sequencing.

text: Adult parthenogenetic females

text: Acyrthosiphon pisum

text: Sfi IA

text: Sfi IB

text: Salivary gland

text: Pea Aphid Salivary Gland

text: LambdaTriplEx2 (Clontech Smart PCR cDNA Library Construction Kit)


text: Genoscope - Centre National de Sequencage

characteristic: INSDC last update

text: live

text: Generic

text: Hirudo medicinalis

text: adult central nervous system

text: Hirudo medicinalis normalized library


text: Max Planck Institute for Infection Biology

text: live

text: Generic

text: plasma cell

characteristic: cell type

text: adult, 7 months of age

characteristic: dev-stage

text: Mus musculus

text: bone marrow

text: Mus musculus bone marrow C57BL/6J adult, 7 months of age


text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: mature naive B cell

text: adult, 7 months of age

text: Mus musculus

text: spleen

text: Mus musculus spleen C57BL/6J adult, 7 months of age


text: live

text: Generic

text: plasma cell

text: adult, 6 months of age

text: Mus musculus

characteristic: organism

text: bone marrow

text: Mus musculus bone marrow B6.129S4-Fcgr2b^tm1Ttk adult, 6 months of age


text: live

text: Generic

text: germinal center B cell

text: adult, 6 months of age

text: Mus musculus

text: spleen

text: Mus musculus spleen B6.129S4-Fcgr2b^tm1Ttk adult, 6 months of age


text: live

text: Generic

text: plasma cell

text: adult, 6 months of age

text: Mus musculus

text: bone marrow

text: Mus musculus bone marrow C57BL/6J adult, 6 months of age


text: live

text: Generic

text: germinal center B cell

text: adult, 6 months of age

text: Mus musculus

text: spleen

text: Mus musculus spleen C57BL/6J adult, 6 months of age


text: Universitiy of Muenster

text: live

text: Generic

text: Race: UVPgt55; isolated from infected wheat leaves 5, 7 dpi

characteristic: description

text: Puccinia graminis f. sp. tritici

text: Library 1 (cDNA of infected wheat leaves minus cDNA of mock-inoculated leaves)


text: Universitiy of Muenster

text: live

text: Generic

text: Race: Ug99; isolated from infected wheat leaves 5, 7 dpi

text: Puccinia graminis f. sp. tritici

text: library 2 (cDNA of infected wheat leaves minus cDNA of mock-inoculated leaves)

characteristic: title


text: live

text: Generic

text: Hirudo medicinalis

text: adult central nervous system

text: Hirudo medicinalis not-normalized library


text: Agricultural Biotechnology Center

text: live

text: Generic

text: Cervus elaphus

text: Cervus elaphus library (Steger V)


text: Leibniz University Hannover

text: live

text: Generic

text: Flammulina velutipes

text: DSMZ 1658

text: mycelium

text: Flammulina velutipes mycelium DSMZ 1658


text: live

text: Generic

text: germinal center B cell

text: adult, 7 months of age

text: Mus musculus

text: spleen

text: Mus musculus spleen B6.129S4-Fcgr2b^tm1Ttk adult, 7 months of age


text: live

text: Generic

text: germinal center B cell

text: adult, 4 months of age

text: Mus musculus

text: spleen

text: Mus musculus spleen B6.129S4-Fcgr2b^tm1Ttk adult, 4 months of age


text: live

text: Generic

text: germinal center B cell

text: adult, 8 months of age

text: Mus musculus

text: spleen

text: Mus musculus spleen B6.129S4-Fcgr2b^tm1Ttk adult, 8 months of age


text: Moredun Research Institute

text: live

text: Generic

text: Teladorsagia circumcincta

text: Teladorsagia circumcincta library (Baker R)


text: Moredun Research Institute

text: live

text: Generic

text: Teladorsagia circumcincta

text: Teladorsagia circumcincta L3


text: IRD (Institut de Recherche pour le Developpement)

text: live

text: Generic

text: Casuarina glauca

text: Non-inoculated root

text: Casuarina glauca library (Hocher V)


text: Kunming University of Science and Technology

text: live

text: Generic

text: Pennong

characteristic: cultivar

text: exposed to 160 mM NO3- for 15 min,1 h, 2 h,6 h,and 24 h

characteristic: lab-host

text: root

text: Spinacia oleracea

text: root

text: Supression Substractive Hybridization cDNA Library of Spinach Root under Nitrate Stress Treatment

text: pMD 18-T Vector


text: Institute Animal Sciences, Chinese Academy of Agricultural Sciences

text: live

text: Generic

characteristic: NCBI submission package

text: Breast muscle

text: 2 weeks; 6 weeks

text: Anas platyrhynchos

text: Female

characteristic: sex

text: White Pekin ducks

text: Breast

text: Anas platyrhynchos muscle-related library constructed by SSH


text: Mahasarakham University; The Agricultural Research Development Agency (Public Organization)

text: live

text: Generic

text: The cDNA was generated as part of Capra hircus project. Total RNA was extracted from cervix of Capra hircus. The total RNA was further purified, reverse transcribed and cloned into p-GEM T easy vector.

text: Capra hircus

text: EcoR I

text: Cervix

text: Capra hircus Cervix Library

text: p-GEM T easy

characteristic: vector


characteristic: External Id

text: Purdue University

text: live

text: Generic

text: Synthesized cDNA was normalized and subsequently amplified using a modified primer containing Not I adapter site. Amplified cDNA was digested using Not I restriction enzyme and ligated into Not I site in pBluescript II SK+ vector. The ligated cDNA was transformed to competent cells.

text: Adult

text: Vigna unguiculata subsp. unguiculata

text: Maruca vitrata

text: Not I

text: Legume pod bore Maruca vitrata expressed sequence tag library from Nigerian samples

text: Plasmid

text: pBluescriptII SK+


text: University of Manitoba

text: live

text: Generic

text: Verticillium dahliae

text: Verticillium dahliae differentially expressed transcripts-cDNA-AFLP-SH-elicitation with potato root extracts


text: National Institute of Allergy and Infectious Diseases

text: live

text: Generic

text: Unidirectional library was constructed using long distance PCR (Smart cDNA kit) and Lambda TriplEx2 vector

text: adults and Vth instar nymphs

text: salivary glands

characteristic: organ

text: Dipetalogaster maximus

text: both

text: Adult and Vth instar salivary gland cDNA library DmaxSG1

text: Phage

text: Lambda TriplEx2


text: Centre de Biotechnonolgie de Borj cedria

characteristic: INSDC center name

text: live

text: Generic

text: Khedhayria

text: Molecular characterization of salt stress in contrasting wild grapevines (Vitis vinifera ssp. sylvestris)

text: Adult

text: Vitis vinifera subsp. sylvestris

text: Leaves

characteristic: tissue-type

text: Vitis vinifera subsp. sylvestris Khedhayria two step RT-PCR library

text: Plasmid

text: pGEM-T Easy vector (Promega)


text: Tamil Nadu Agricultural University

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Thirty five days old cultivated and wild genotypes of tomato plants were spray inoculated with early blight pathogen, Alternaria solani and plants sprayed with double distilled water served as control plants. Total RNA was extracted from the leaf tissues collected at 6h and 24h post inoculation from the control and treated plants by TRI-reagent method. DD-RT-PCR was performed to identify the differentially expressed cDNAs during different treatments. Differentially expressed cDNA fragments were cloned in pTZ57R/T vector (MBI Fermentas) and sequenced by Automated sequencer. The sequence information was annotated to identify the putative function of the differentially expressed genes against sequences deposited in NCBI and TIGR databases. || TYPE: Pub TITLE: Expressed sequence tags of cDNA clones from tomato leaves challenged with pathogen Alternaria solani AUTHORS: Abida,P.S.; Krishnaveni,S.; Madhura,J.N.; Nataraja,K.N.; Raveendran,M.; Velazhahan,R.; Krishnasamy,V.; Sajeevan,R.S.; Marimuthu,M.; Palanisami,J.R. JOURNAL: VOLUME: ISSUE: PAGES: YEAR: 2010 STATUS: 1

text: E. coli

text: Solanum lycopersicum

text: Eco RI

text: Eco RI

text: Leaf

text: Alternaria solani challenged tomato leaf cDNA library through DD-RT-PCR

text: pTZ57R/T vector


text: College of Horticulture, Northwest A&F University

text: live

text: Generic

text: Growing stage

characteristic: dev-stage

text: E.coli DH5a

text: Vitis quinquangularis

characteristic: strain

text: young leaf

text: Young leaves inoculated with Elsinoe ampelina (anthracnose of grape) SSH cDNA library of Chinese Wild Vitis quinquangularis

text: pGEM-T Easy Vector


text: International Center for Tropical Agriculture, CIAT. http://www.ciat.cgiar.org

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Total RNA was prepared from common bean roots subjected to low or high phosphorus conditions in a hydroponic chamber in the greenhouse of Pennsylvania State University, USA; poly(A) RNA was purified and reverse transcribed at the the International Center for Tropical Agriculture (CIAT), Cali, COLOMBIA, and cDNAs were cloned into UniZAP XR vector arms. The library was transformed into E. coli cells which were plated on petri plates with Carbenicillin anti-biotic (100 mg/L) and selected by blue-white screening for inserts. A Q-Bot was used to pick and array colonies into plates and filters.

text: E. coli XL-1-Blue MRF'

text: Phaseolus vulgaris

text: EcoRI

text: XhoI

text: Root

text: Phaseolus vulgaris low phosphorous root EST library

text: Plasmid

text: UniZAP XR


text: live

text: Generic

characteristic: description

text: E. coli XL-1-Blue MRF'

text: Phaseolus vulgaris

characteristic: organism

text: EcoRI

text: XhoI

text: Root

text: Phaseolus vulgaris high phosphorous root EST library

text: Plasmid

text: UniZAP XR


text: College of Life Science & Biotechnology, Korea University

text: live

text: Generic

text: The library was constructed from poly (A+) RNA using BD Creator SMART cDNA library construction kit from Clone Tech. The cDNAs were directionally excised as inserts into the sfi/sfi sites in plasmid pLIB of Molony murine Leukemia virus (MMLV) by using DNA Ligase III. The library represents 1.509 x 103 independent recombinant phage. Selection marker: Ampicilin/Chloroamphenicol

text: 4-6 month old

text: E. coli DH5 alpha (RBC. Taiwan)

text: Oryzias latipes

text: sfiI

text: male

text: d-rR (orange-red)

text: liver

text: Japanese Medaka Hepatic cDNA Library

characteristic: title

text: Retroviral plasmid

text: pLIB


text: National Academy of Agricultural Science

text: live

text: Generic

text: cDNA was inserted between attB1 and attB2 of pDONR222 vector at 5'- and 3'-end with BsrGI.

text: adult

text: E.coli DH10B

characteristic: lab-host

text: Muraenesox cinereus

text: BsrGI

text: liver

text: Eel liver cDNA library

text: plasmid


text: CIIDIR-IPN Unidad Sinaloa

text: live

text: Generic

characteristic: NCBI submission package

text: Fianna

characteristic: cultivar

text: original sequencing of Solanum tuberosum variety Fianna generating a subtractive cDNA library using the method of suppression subtractive hybridization (SSH); Internodes and veins of leaves of potato plants were used in vitro non-infected and infected by Mexican potato purple top phytoplasma

text: Solanum tuberosum

text: internodes and veins of leaves from potato plant In vitro

text: Solanum tuberosum cDNA library in response to phytoplasma infection

text: pDrive cloning vector 3.85 Kb


text: Dong-A University

text: live

text: Generic

text: Stratagene (cat. #200450); Total RNA was extracted separately from leaf tissues, using the method described by Chung et al. (2009). mRNA was reverse transcribed into double stranded cDNA using a modified oligo (dT)18 primer with an identifying tag sequence. Double stranded cDNAs were size selected (more then 500 bp), adaptored with EcoRI adapters at both ends and then digested with XhoI. The cDNAs were then directionally cloned into EcoRI-XhoI digested pBSII SK(+) phagemid vector (Stratagene). The total number of white colony forming units (cfu) was 0.8x100,000,000 cfu. The background of empty clones was less than 10%. Inserts ranged from 0.3 kb to 5kb, as determined by PCR.

text: mature

text: E. coli SOLR strain

text: leaf

text: Raphanus sativus var. niger

text: salt stress treated wild radish leaf library


text: Northeast Forestry University

text: live

text: Generic

text: Suk

text: 10-12 days before the formation of sporogonium

text: male inflorescence

text: Betula platyphylla

text: male

characteristic: sex

text: Birch male inflorescence's early development cDNA-AFLP library


characteristic: External Id

text: USDA-National Center for Agricultural Utilization Research

text: live

text: Generic

text: Set of 46 cDNAs from Coniochaeta ligniaria NRRL30616

text: Coniochaeta ligniaria

text: 5' EcoRI

text: 3' XhoI

text: Coniochaeta ligniaria NRRL30616 cDNA library

text: plasmid

characteristic: vector


text: Sri Krishnadevaraya University

characteristic: INSDC center name

text: live

text: Generic

text: Surface sterilized seeds germinated in plastic pots contained soil and sand (2:1) mixture and allowed to grow under natural photoperiod (10-12 hrs, 28 +/- 4degC) in the departmental botanic garden. Seedlings were maintained at 100% soil moisture levels (SMLs) by watering daily. Nineteen day-old plants were subjected to drought stress by with holding water till wilting. Leaf samples collected from drought stressed plants, flash frozen and stored at -80degC; Total RNA was extracted from the plant tissues with the Trizol reagent, and then was treated with DNase (TurboDNAse, Ambion, USA) for removing the potential genomic DNA contamination. The first-strand cDNA was synthesized with the RevertAid Hminus first strand cDNA synthesis kit (Fermentas, Germany) according to the manufacture?s protocol. PCR product using gene specific primers was amplified and cloned into InsTA PCR cloning kit (pTZ57R) (Fermentas, Germany), transformed to DH5alpha (Invitrogen, Germany). Plasmid DNA was purified and sequenced.

text: 21 day-old plants

text: E.coli

text: Macrotyloma uniflorum

text: EcoRI

text: HindIII

text: Leaf

text: Stress responsive NAC TF family genes in horsegram (Macrotylomauniflorum (Lam.)Verdc.)


text: The University of Tokyo

text: live

text: Generic

text: Osmunda lancea

text: leaf

characteristic: tissue-type

text: Osmunda lancea leaf


text: Forestry and Forest Products Research Institute

characteristic: INSDC first public

text: live

text: Generic

text: seedling

text: Sciadopitys verticillata

text: Sciadopitys verticillata seedling cDNA library


text: University Bremen, Dept. of Microbe-Plant-Interactions

characteristic: INSDC last update

text: live

text: Generic

text: IRGC 110404

text: Xa21 transcriptome, direct sequencing of cDNA (pyrosequencing) without cloning steps

text: Grown- up stage, not flowering

text: Oryza longistaminata

text: Roots

text: Oryza longistaminata cultivar IRGC 110404 Xa21 transcriptome


text: Faculty of Horticulture, Uttar Banga Krishi Viswavidyalaya (North Bengal Agricultural University)

text: live

text: Generic

text: According to the instructions given in Clontech Manual on SMART cDNA Library Construction Kit Manual (No. 634901; Clontech Lab Inc, USA)

text: Camellia sinensis var. assamica

text: Root

text: TEAFLUBKV


text: Tea Research Association

text: live

text: Generic

text: Clone P1258

text: According to the instructions given in Clontech PCR-Select cDNA Subtraction Kit user manual (No. 637401; Clontech Lab Inc, USA)

text: Leaf

characteristic: organ

text: Camellia sinensis

text: Camellia sinensis ALP1258 pGEM-T cDNA library

text: Plasmid

text: pGEM-T


text: Tea Research Association

text: live

text: Generic

text: Clone P1258

text: According to the instructions given in Clontech Creator Smart cDNA Library Construction Kit user manual (Cat. No. 634903; Clontech Lab Inc, USA)

text: Leaf

text: Camellia sinensis

text: Camellia sinensis ALP1258 pDNBR cDNA library

text: Plasmid

text: pDNBR


text: Tea Research Association

text: live

text: Generic

text: Clone 19/22/4

text: According to the instructions given in Clontech PCR-Select cDNA Subtraction Kit user manual (Cat. No. 637401; Clontech Lab Inc, USA)

text: Leaf

text: Camellia sinensis var. assamica

text: Camellia assamica PA1 cDNA library

text: Plasmid

text: pGEM-T


text: Tea Research Association

text: live

text: Generic

text: Clone P1258

text: Leaf

text: Camellia sinensis

text: Camellia sinensis BLP1258 pGEM-T cDNA library

text: Plasmid

text: pGEM-T


text: Tea Research Association

text: live

text: Generic

text: Clone T78

text: Leaf

text: Camellia sinensis

text: Camellia sinensis BLT78 cDNA library

text: Plasmid

text: pGEM-T


text: Tea Research Association

text: live

text: Generic

text: Clone T78

text: Leaf

text: Camellia sinensis

text: Camellia sinensis ALT78 cDNA library

text: Plasmid

text: pGEM-T


text: Tea Research Association

text: live

text: Generic

text: Clone S.3A/3

text: Leaf

text: Camellia sinensis var. assamica

text: Camellia assamica BWS3A3 cDNA library

text: Plasmid

text: pGEM-T


text: Tea Research Association

text: live

text: Generic

text: Clone TV23

text: Leaf

text: Camellia sinensis var. assamica

text: Camellia assamica ESTBW23E3E4 cDNA library

text: Plasmid

text: pGEM-T


text: Tea Research Association

text: live

text: Generic

text: Clone TV23

text: leaf

text: Camellia sinensis var. assamica

text: Camellia assamica W23E3E4 cDNA library

text: Plasmid

text: pGEM-T


text: Tea Research Association

text: live

text: Generic

text: Clone S.3A/3

text: Leaf

text: Camellia sinensis var. assamica

text: Camellia assamica WS3A3 cDNA library

text: Plasmid

text: pGEM-T


text: Tea Research Association

text: live

text: Generic

text: Clone TV23

text: Leaf

text: Camellia sinensis var. assamica

text: Camellia assamica WTV23CTV23E cDNA library

text: Plasmid

text: pGEM-T


text: Tea Research Association

text: live

text: Generic

text: Clone TV23

text: Leaf

text: Camellia sinensis var. assamica

text: Camellia assamica WTV23ETV23C cDNA library

text: Plasmid

text: pGEM-T


text: Tea Research Association

text: live

text: Generic

text: Clone TV23

text: Leaf

text: Camellia sinensis var. assamica

text: Camellia assamica BWSSH1TFL cDNA library

text: Plasmid

text: pGEM-T


text: Tea Research Association

text: live

text: Generic

text: Clone S3A3

text: Leaf

text: Camellia sinensis var. assamica

text: Camellia assamica BWSSH2TFL cDNA library

text: Plasmid

text: pGEM-T


text: Tea Research Association

text: live

text: Generic

text: Clone TV23

text: Leaf

text: Camellia sinensis var. assamica

text: Camellia assamica WSSH3TFL cDNA library

text: Plasmid

text: pGEM-T


text: Tea Research Association

text: live

text: Generic

text: According to the instructions in Clonetech PCR-Select cDNA subtraction Kit manual (Cat. No.637401)

text: Leaf

text: Camellia sinensis

text: Camellia sinensis DQMI1 cDNA library

text: Plasmid

text: pGEM-T


text: Tea Research Association

text: live

text: Generic

text: Clone H311

text: According to instructions given in PCR select cDNA subtraction kit manual, cat no. 637401, Clontech corporation

text: Leaf

text: Camellia sinensis

text: Camellia sinensis AQMI1 cDNA library

text: plasmid

text: pGEM-T


text: Tea Research Association

text: live

text: Generic

text: Clone 615.28.3

text: Leaf

text: Camellia sinensis var. assamica

text: Camellia assamica SKDCC1 cDNA library

text: plasmid

text: pGEM-T


text: Tea Research Association

text: live

text: Generic

text: Clone S.3A/3

text: In accordance with the reccommendations in the PCR select cDNA subtraction kit user manual, cat no. 637401, Clontech corporation.

text: Leaf

text: Camellia sinensis var. assamica

text: Camellia assamica S2 cDNA library

text: plasmid

text: pGEM-T


text: Tea Research Association

text: live

text: Generic

text: Clone 111/1

text: According to the instructions given in PCR select cDNA subtraction kit user manual, cat no. 637401, Clontech corporation.

text: Leaf

text: Camellia sinensis var. assamica

text: Camellia assamica S3 cDNA library

text: plasmid

text: pGEM-T


text: Tea Research Association

text: live

text: Generic

text: clone 111/1

text: Leaf

text: Camellia sinensis var. assamica

text: Camellia assamica S4 cDNA library

text: plasmid

text: pGEM-T


text: Tea Research Association

text: live

text: Generic

text: Clone TV1

text: According to the instructions in user manual for PCR select cDNA subtraction kit, cat no. 637401, Clontech corporation.

text: Leaf

text: Camellia sinensis var. assamica

text: Camellia assamica S5 cDNA library

text: plasmid

text: pGEM-T


text: Instituto de Quimica da UFRJ

text: live

text: Generic

text: Unidirectional library was constructed using long distance PCR (Smart cDNA kit) and Lambda TriplEx2 vector

text: adult

characteristic: dev-stage

text: ovarian epithelium

text: Rhodnius prolixus

text: female

text: Rhodnius prolixus adult females folicular epithelium cDNA library

text: Phage

text: Lambda TriplEx2


text: National Institute of Genetics

text: live

characteristic: INSDC status

text: Generic

text: segmentation stage 20 - 25

text: Oryzias latipes

text: mixture of female and male

text: Hd-rR

characteristic: strain

text: whole embryo

text: MF01SSA cDNA


text: National Institute of Genetics

text: live

text: Generic

characteristic: NCBI submission model

text: stage 15

text: Xenopus laevis

text: whole embryo

text: NIBB Mochii normalized Xenopus neurula library


text: Chinese Academy of Medical Sciences & Peking Union Medical College

text: live

text: Generic

text: fetal

text: Homo sapiens

characteristic: organism

text: hippocampus

text: Human fetal hippocampus cDNA library


text: National Institute of Genetics

text: live

text: Generic

text: stage 25

text: Xenopus laevis

text: whole embryo

text: NIBB Mochii normalized Xenopus tailbud library

characteristic: title


text: National Institute of Genetics

text: live

text: Generic

text: stage 10.5

text: Xenopus laevis

text: whole embryo

text: NIBB Mochii normalized Xenopus early gastrula library


text: Research Institute of Field Surgery, Daping Hospital, Third Military Medical University

text: live

text: Generic

text: brain

text: Mus musculus

text: Male

text: BALB/c

text: Mice brain suppression subtractive hybridization library


text: Osaka Prefecture University, Postharvest Physiology and Quality Control

text: live

text: Generic

text: Kyoho

text: veraison

text: Vitis labrusca

text: Uni-Zap XR grape berry cDNA


text: Michigan State University

text: live

text: Generic

text: 45 d and 90 d of gestation, birth, 7 wk and 1 yr of age

characteristic: lab-host

text: hind limbs

text: Sus scrofa

text: NotI

text: SalI

text: male and female

text: skeletal muscles

text: porcine skeletal muscle cDNA library (PoSM)

text: plasmid


text: Peking Union Medical College Hospital

text: live

text: Generic

characteristic: NCBI submission package

text: total RNA isolated from pheochromocytoma and adjacent adrenal gland were reverse transcribed and amplified with DD-RT-PCR, differentially displayed bands were subcloned into pGEM-T easy vector and sequenced.

characteristic: description

text: Homo sapiens

text: adrenal gland

text: adrenal gland (DD-RT-PCR vs PHEO)

text: Plasmid

text: pGEM-T easy


text: Peking Union Medical College Hospital

text: live

text: Generic

text: Homo sapiens

text: pheochromocytoma

text: PHEO (DD-RT-PCR vs adrenal gland)

text: Plasmid

text: pGEM-T easy


text: Centro de Invest. Cientifica de Yucata

text: live

text: Generic

text: country=Mexico:Yucatan

text: Coffea arabica

text: cell suspension culture

text: Coffea arabica cell suspension culture


text: Centro de Invest. Cientifica de Yucata

text: live

text: Generic

text: country=Mexico:Yucatan

text: Coffea arabica

text: callus

text: Coffea arabica callus


text: Radiation and Nuclear Safety Authority

text: live

text: Generic

text: epithelial

characteristic: cell type

characteristic: cell-line

text: Homo sapiens

text: thyroid

text: Homo sapiens S284N


characteristic: External Id

text: Radiation and Nuclear Safety Authority

text: live

text: Generic

text: epithelial

text: Homo sapiens

text: thyroid tumour

text: Homo sapiens S284T


text: Radiation and Nuclear Safety Authority

characteristic: INSDC center name

text: live

text: Generic

text: epithelial

text: Homo sapiens

text: thyroid tumour

text: Homo sapiens S285T


text: Institut National de la Recherche Agronomique

text: live

text: Generic

text: granulosa

text: immature

text: Sus scrofa

text: female

characteristic: sex

text: ovary

characteristic: tissue-type

text: Sus scrofa ovary immature


text: Centenary Institute

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Liver

text: Homo sapiens

text: SSH Library from Adult Cirrhotic Liver

text: Plasmid

text: pGEM T Easy II (Promega)

characteristic: vector


text: National Institute on Aging/National Institutes of Health

text: live

characteristic: INSDC status

text: Generic

text: Mouse cDNA project by the Laboratory of Genetics, National Institute on Aging (NIA), Intramural Research Program, NIH (http://lgsun.grc.nia.nih.gov/cDNA).FACS-sorted Germinal Center B cells were provided by Drs. Richard Hodes, Emily Klotz (National Institute on Aging and National Cancer Institute, USA) and Garnett Kelsoe (Duke University, USA). Double-stranded cDNAs were synthesized from 0.46 ug of total RNA with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'], treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal3 (Ref. Development 127: 1737-1749 (2000) [PMID: 10725249]), purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) and purified by phenol/chloroform, followed by Centricon 100 purification. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pSPORT1 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is 1.2 kb. The library was constructed by Yulan Piao (NIA).

text: Mus musculus

text: SalI

text: NotI

text: Germinal Center B Cell

text: NIA Mouse Germinal Center B Cell cDNA Library

text: plasmid (ampicillin resistant)

text: pSPORT1 (Invitrogen)


text: live

text: Generic

text: R1 ES cells

text: Mouse cDNA project by the Laboratory of Genetics, National Institute on Aging (NIA), Intramural Research Program, NIH (http://lgsun.grc.nia.nih.gov/cDNA).This is a long-transcript enriched cDNA library (Ref. Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]). Total RNAs were obtained from Dr. Kenneth R. Boheler (National Institute on Aging, USA). ES cells were cultured without feeder cells in the presence of LIF and BRL-conditioned media. Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 14.2 ug of total RNA, treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-S. The products were purified by phenol/chloroform and Centricon 100. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pSPORT1 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 2.4 kb. The library was constructed by Yulan Piao (NIA).

text: Mus musculus

text: SalI

text: NotI

text: 129/Sv x 129/Sv-CP

characteristic: strain

text: Undifferentiated ES Cell

text: NIA Mouse Undifferentiated ES Cell cDNA Library (Long)

text: plasmid (ampicillin resistant)

text: pSPORT1 (Invitrogen)


text: live

text: Generic

characteristic: NCBI submission model

text: Mouse cDNA project by the Laboratory of Genetics, National Institute on Aging (NIA), Intramural Research Program, NIH (http://lgsun.grc.nia.nih.gov/cDNA). This is a long-transcript enriched cDNA library (Ref. Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]). Total RNAs were obtained from Dr. Janet Rossant and Tilo Kunath (Samuel Lunenfeld Research Institute, Canada). Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 4 ug of total RNA, treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-S. The products were purified by phenol/chloroform and Centricon 100. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pSPORT1 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 2.6 kb. The library was constructed by Yulan Piao (NIA).

characteristic: dev-stage

text: Mus musculus

text: SalI

text: NotI

text: B5/EGFP transgenic ICR mice

text: Trophoblast stem cell

text: NIA Mouse Trophoblast Stem Cell cDNA Library (Long)

text: plasmid (ampicillin resistant)

text: pSPORT1 (Invitrogen)


text: live

text: Generic

text: Mouse cDNA project by the Laboratory of Genetics, National Institute on Aging (NIA), Intramural Research Program, NIH (http://lgsun.grc.nia.nih.gov/cDNA). This is a short-transcript enriched cDNA library (Ref. Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]). Total RNAs were obtained from Dr. Janet Rossant and Tilo Kunath (Samuel Lunenfeld Research Institute, Canada). Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 4 ug of total RNA, treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-L. The products were purified by phenol/chloroform and Centricon 100. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pSPORT1 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 1.3 kb. The library was constructed by Yulan Piao (NIA).

text: Mus musculus

characteristic: organism

text: SalI

text: NotI

text: B5/EGFP transgenic ICR mice

text: Trophoblast stem cell

text: NIA Mouse Trophoblast Stem Cell cDNA Library (Short)

characteristic: title

text: plasmid (ampicillin resistant)

text: pSPORT1 (Invitrogen)


text: live

text: Generic

characteristic: NCBI submission package

text: Mouse cDNA project by the Laboratory of Genetics, National Institute on Aging (NIA), Intramural Research Program, NIH (http://lgsun.grc.nia.nih.gov/cDNA). This is a long-transcript enriched cDNA library (Ref. Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]). Total RNAs were extracted from a pool of 20 Blastocysts. Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 0.2 ug of total RNA, treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-S. The products were purified by phenol/chloroform and Centricon 100. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pSPORT1 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 2.2 kb. The library was constructed by Yulan Piao (NIA).

characteristic: lab-host

text: Mus musculus

text: SalI

text: NotI

text: Blastocyst

text: NIA Mouse Blastocyst cDNA Library (Long)

text: plasmid (ampicillin resistant)

text: pSPORT1 (Invitrogen)


text: live

text: Generic

text: Mouse cDNA project by the Laboratory of Genetics, National Institute on Aging (NIA), Intramural Research Program, NIH (http://lgsun.grc.nia.nih.gov/cDNA). This is a long-transcript enriched cDNA library (Ref. Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]). Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 1.8 ug of total RNA, treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-S. The products were purified by phenol/chloroform and Centricon 100. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pSPORT1 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 2.4 kb. The library was constructed by Yulan Piao (NIA).

characteristic: description

text: Mus musculus

text: SalI

text: NotI

text: Male

text: Male genital ridge/mesonephros

text: NIA Mouse 12.5-dpc Male Genital Ridge/Mesonephros cDNA Library (Long)

text: plasmid (ampicillin resistant)

text: pSPORT1 (Invitrogen)


text: live

text: Generic

text: Mouse cDNA project by the Laboratory of Genetics, National Institute on Aging (NIA), Intramural Research Program, NIH (http://lgsun.grc.nia.nih.gov/cDNA). This is a long-transcript enriched cDNA library (Ref. Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]). Total RNAs were obtained from Drs. Dennis Taub, Dan Longo (National Institute on Aging, USA), Jonathan Keller (National Cancer Institute, USA). Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 2.4 ug of total RNA, treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-S. The products were purified by phenol/chloroform and Centricon 100. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pSPORT1 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 2.2 kb. The library was constructed by Yulan Piao (NIA).

text: Age approx.10 weeks old

text: Mus musculus

text: SalI

text: NotI

text: NIA Mouse Hematopoietic Stem Cell (Lin-/c-Kit+/Sca-1-) cDNA Library (Long)

text: plasmid (ampicillin resistant)

text: pSPORT1 (Invitrogen)


text: live

text: Generic

text: Mouse cDNA project by the Laboratory of Genetics, National Institute on Aging (NIA), Intramural Research Program, NIH (http://lgsun.grc.nia.nih.gov/cDNA). This is a long-transcript enriched cDNA library (Ref. Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]). Total RNAs were extracted from a pool of 1488 unfertilized eggs. Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'], treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-S. The products were purified by phenol/chloroform and Centricon 100. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pSPORT1 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 2.5 kb. The library was constructed by Yulan Piao (NIA).

text: Mus musculus

text: SalI

text: NotI

text: Unfertilized Egg

text: NIA Mouse Unfertilized Egg cDNA Library (Long)

text: plasmid (ampicillin resistant)

text: pSPORT1 (Invitrogen)


text: National Institute on Aging/National Institutes of Health

text: live

text: Generic

text: KUSA-A1 cells

characteristic: cell-line

text: Mouse cDNA project by the Laboratory of Genetics, National Institute on Aging (NIA), Intramural Research Program, NIH (http://lgsun.grc.nia.nih.gov/cDNA). This is a long-transcript enriched cDNA library (Ref. Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]). Total RNAs were obtained from Dr. Akihiro Umezawa (Keio University School of Medicine, Japan). Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 2.1 ug of total RNA, treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-S. The products were purified by phenol/chloroform and Centricon 100. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pSPORT1 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 3.0 kb. The library was constructed by Yulan Piao (NIA).

text: Mus musculus

text: SalI

text: NotI

text: Osteoblast

text: NIA Mouse Osteoblast cDNA Library (Long)

text: plasmid (ampicillin resistant)

text: pSPORT1 (Invitrogen)


characteristic: External Id

text: live

text: Generic

text: 9-15-C cells

text: Mouse cDNA project by the Laboratory of Genetics, National Institute on Aging (NIA), Intramural Research Program, NIH (http://lgsun.grc.nia.nih.gov/cDNA). This is a long-transcript enriched cDNA library (Ref. Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]). Total RNAs were obtained from Dr. Akihiro Umezawa (Keio University School of Medicine, Japan). Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 2.2 ug of total RNA, treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-S. The products were purified by phenol/chloroform and Centricon 100. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pSPORT1 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 2.5 kb. The library was constructed by Yulan Piao (NIA).

text: Mus musculus

text: SalI

text: NotI

text: Mesenchymal stem cell

text: NIA Mouse Mesenchymal Stem Cell cDNA Library (Long)

text: plasmid (ampicillin resistant)

text: pSPORT1 (Invitrogen)


characteristic: INSDC center name

text: live

text: Generic

text: Mouse cDNA project by the Laboratory of Genetics, National Institute on Aging (NIA), Intramural Research Program, NIH (http://lgsun.grc.nia.nih.gov/cDNA). This is a long-transcript enriched cDNA library (Ref. Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]). Total RNAs were obtained from Drs. Dennis Taub, Dan Longo (National Institute on Aging, USA), Jonathan Keller (National Cancer Institute, USA). Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 4.8 ug of total RNA, treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-S. The products were purified by phenol/chloroform and Centricon 100. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pSPORT1 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 2.7 kb. The library was constructed by Yulan Piao (NIA).

text: Age approx.10 weeks old

text: Mus musculus

text: SalI

text: NotI

text: NIA Mouse Hematopoietic Stem Cell (Lin-/c-Kit+/Sca-1+) cDNA Library (Long)

text: plasmid (ampicillin resistant)

text: pSPORT1 (Invitrogen)


text: live

text: Generic

text: R1 ES cells

text: Mouse cDNA project by the Laboratory of Genetics, National Institute on Aging (NIA), Intramural Research Program, NIH (http://lgsun.grc.nia.nih.gov/cDNA). This is a short-transcript enriched cDNA library (Ref. Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]). Total RNAs were obtained from Dr. Kenneth R. Boheler (National Institute on Aging, USA). ES cells were cultured without feeder cells in the presence of LIF and BRL-conditioned media. Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 14.2 ug of total RNA, treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-L. The products were purified by phenol/chloroform and Centricon 100. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pSPORT1 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 1.4 kb. The library was constructed by Yulan Piao (NIA).

text: Mus musculus

text: SalI

text: NotI

text: 129/Sv x 129/Sv-CP

text: Undifferentiated ES Cell

text: NIA Mouse Undifferentiated ES Cell cDNA Library (Short)

text: plasmid (ampicillin resistant)

text: pSPORT1 (Invitrogen)


text: live

text: Generic

text: R1 ES cells

text: Mouse cDNA project by the Laboratory of Genetics, National Institute on Aging (NIA), Intramural Research Program, NIH (http://lgsun.grc.nia.nih.gov/cDNA). This is a long-transcript enriched cDNA library (Ref. Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]). Total RNAs were obtained from Dr. Kenneth R. Boheler (National Institute on Aging, USA). ES cells were cultured without feeder cells in the absence of LIF for 4 hours or 18 hours. Equimolar mixture of these RNA samples was used for the library construction. Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 21 ug of total RNA, treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-S. The products were purified by phenol/chloroform and Centricon 100. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pSPORT1 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 2.4 kb. The library was constructed by Yulan Piao (NIA).

text: Mus musculus

text: SalI

text: NotI

text: 129/Sv x 129/Sv-CP

text: ES Cell (LIF-)

text: NIA Mouse ES Cell (LIF-) cDNA Library (Long)

text: plasmid (ampicillin resistant)

text: pSPORT1 (Invitrogen)


text: live

text: Generic

text: R1 ES cells

text: Mouse cDNA project by the Laboratory of Genetics, National Institute on Aging (NIA), Intramural Research Program, NIH (http://lgsun.grc.nia.nih.gov/cDNA). This is a short-transcript enriched cDNA library (Ref. Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]). Total RNAs were obtained from Dr. Kenneth R. Boheler (National Institute on Aging, USA). ES cells were cultured without feeder cells in the absence of LIF for 4 hours or 18 hours. Equimolar mixture of these RNA samples was used for the library construction. Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 21 ug of total RNA, treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-L. The products were purified by phenol/chloroform and Centricon 100. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pSPORT1 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 1.3 kb. The library was constructed by Yulan Piao (NIA).

text: Mus musculus

text: SalI

text: NotI

text: 129/Sv x 129/Sv-CP

text: ES Cell (LIF-)

text: NIA Mouse ES Cell (LIF-) cDNA Library (Short)

text: plasmid (ampicillin resistant)

text: pSPORT1 (Invitrogen)


text: live

text: Generic

text: Mouse cDNA project by the Laboratory of Genetics, National Institute on Aging (NIA), Intramural Research Program, NIH (http://lgsun.grc.nia.nih.gov/cDNA). This is a long-transcript enriched cDNA library (Ref. Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]). Total RNAs were extracted from a pool of four embryos at 7.5-days postcoitum. Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 7 ug of total RNA, treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-S. The products were purified by phenol/chloroform and Centricon 100. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pSPORT1 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 2.2 kb. The library was constructed by Yulan Piao (NIA).

text: 7.5-days postcoitum

text: Mus musculus

text: SalI

text: NotI

text: whole embryo including extraembryonic tissues at 7.5-days postcoitum

text: NIA Mouse 7.5-dpc Whole Embryo cDNA Library (Long)

text: plasmid (ampicillin resistant)

text: pSPORT1 (Invitrogen)


text: live

text: Generic

text: Mouse cDNA project by the Laboratory of Genetics, National Institute on Aging (NIA), Intramural Research Program, NIH (http://lgsun.grc.nia.nih.gov/cDNA). This is a long-transcript enriched cDNA library (Ref. Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]). Total RNAs were obtained from Drs. Dennis Taub, Dan Longo (National Institute on Aging, USA), Jonathan Keller (National Cancer Institute, USA). Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 1.1 ug of total RNA, treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-S. The products were purified by phenol/chloroform and Centricon 100. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pSPORT1 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 2.2 kb. The library was constructed by Yulan Piao (NIA).

text: Age approx.10 weeks old

text: Mus musculus

text: SalI

text: NotI

text: NIA Mouse Hematopoietic Stem Cell (Lin-/c-Kit-/Sca-1+) cDNA Library (Long)

text: plasmid (ampicillin resistant)

text: pSPORT1 (Invitrogen)


text: National Institute on Aging/National Institutes of Health

text: live

text: Generic

text: Mouse cDNA project by the Laboratory of Genetics, National Institute on Aging (NIA), Intramural Research Program, NIH (http://lgsun.grc.nia.nih.gov/cDNA). This is a long-transcript enriched cDNA library (Ref. Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]). Total RNAs were obtained from Drs. Dennis Taub, Dan Longo (National Institute on Aging, USA), Jonathan Keller (National Cancer Institute, USA). Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 0.9 ug of total RNA, treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-S. The products were purified by phenol/chloroform and Centricon 100. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pSPORT1 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 2.1 kb. The library was constructed by Yulan Piao (NIA).

text: Age approx.10 weeks old

text: Mus musculus

text: SalI

text: NotI

text: NIA Mouse Hematopoietic Stem Cell (Lin-/c-Kit-/Sca-1-) cDNA Library (Long)

text: plasmid (ampicillin resistant)

text: pSPORT1 (Invitrogen)


text: live

text: Generic

text: Mouse cDNA project by the Laboratory of Genetics, National Institute on Aging (NIA), Intramural Research Program, NIH (http://lgsun.grc.nia.nih.gov/cDNA). This is a long-transcript enriched cDNA library (Ref. Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]). Total RNAs were extracted from a pool of 13 embryos at 8.5-days postcoitum. Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 9.1 ug of total RNA, treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-S. The products were purified by phenol/chloroform and Centricon 100. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pSPORT1 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 2.5 kb. The library was constructed by Yulan Piao (NIA).

text: 8.5-days postcoitum

text: Mus musculus

text: SalI

text: NotI

text: whole embryo including extraembryonic tissues at 8.5-days postcoitum

text: NIA Mouse 8.5-dpc Whole Embryo cDNA Library (Long)

text: plasmid (ampicillin resistant)

text: pSPORT1 (Invitrogen)


text: Portland VA Medical Center

text: live

text: Generic

text: Differential display analysis was carried out on RNA extracted from mouse whole brain following a chronic exposure to ethanol

text: Brain

characteristic: organ

text: Mus musculus

text: Male

characteristic: sex

text: Differential display analysis of chronic ethanol exposure in mouse brain


text: West Virginia University

characteristic: INSDC first public

text: live

text: Generic

text: peripheral blood leukocytes

characteristic: cell type

text: mid-lactation

text: Bos taurus

text: NotI

text: SalI

text: female

text: Blood

characteristic: tissue-type

text: Normalized Bovine Total Leukocyte cDNA Library (BOTL)

text: plasmid


text: US Department of Agriculture (USDA), ARS, PSI

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: The K-plate contains genomic and cDNA clones, in part, used as markers on our soybean map. Some of the cDNA clones were from differential display, while others were collected from unrelated soybean studies. Cloning vectors vary in this plate and include pT& blue, pIBI30, pIBI31, pUC18, pUC119 and pBluescript SK- phagemid.

text: Glycine max

text: K Plate


text: INRA

text: live

text: Generic

characteristic: NCBI submission model

text: The cDNA library was constructed from total RNA of Tuber borchii mycelium grown on PDA agar medium using the UniZapXR cDNA library System Construction kit (Stratagene, USA) according to the manufacturer's instructions. UniZapXR clones were converted to pBK-CMV phagemid clones using E. coli BM25.8 as the bacterial host

text: vegetative hyphae

text: mycelium

text: Tuber borchii

text: EcoRI

text: mycelium

text: cDNA library of Tuber borchii vegetative mycelium grown on PDA agar medium

text: pBK-CMV phagemid

characteristic: vector


text: Dipartimento di Biologia Vegetale, Universita degli Studi di Torino

text: live

text: Generic

text: The cDNA library was constructed from total RNA of Tuber borchii mycelium grown on MMN liquid medium using the UniZapXR cDNA library System Construction kit (Stratagene, USA) according to the manufacturer's instructions. UniZapXR clones were converted to pBK-CMV phagemid clones using E. coli BM25.8 as the bacterial host

text: vegetative hyphae

characteristic: dev-stage

text: mycelium

text: Tuber borchii

characteristic: organism

text: EcoRI

text: mycelium

text: cDNA library of Tuber borchii vegetative mycelium grown on MMN liquid medium

text: pBK-CMV phagemid


text: Telethon Foundation/university of Pisa (Italy)

text: live

text: Generic

characteristic: NCBI submission package

text: XL1-Blue MRF'

text: testis

text: Mus musculus

text: Xho I

text: Eco R1

text: Testis, Stratagene (cat. #937301)

characteristic: title

text: Phage

text: Uni-Zap XR


text: Institute of Basic Medicine

text: live

text: Generic

text: Homo sapiens

text: lung

text: Homo sapiens pla-801c


text: Washington University School of Medicine

text: live

text: Generic

text: The library was constructed by R Haywood. cDNAs were synthesized from ganetocyte poly(A)+ RNA by oligo d(T) priming, size-selected and directionally cloned into the EcoRi (5' end) to XhoI (3' end) sites of the Uni-ZAP XR lambda vector (Stratagene). The primary library was mass excised as phagemid using the ExAssist helper phage (Stratagene). Clones were mass excised using the ExAssist helper phage (Stratagene), the phagemids were precitptated with PEG 8000 and extracted with phenol/chloroform. Phagemid DNA was electroporated into DH10B cells. Clone Availability: David Sibley, Washington University.

characteristic: description

text: gametocyte (stage III-V)

text: DH10B (GeneHog, Invitrogen, Inc.)

characteristic: lab-host

text: Plasmodium falciparum 3D7

text: EcoRI

text: XhoI

text: Plasmodium falciparum 3D7 gametocyte cDNA library

text: phagemid (ampicillin resistant)

text: pBluescript SK plus


characteristic: External Id

text: INRA

text: live

text: Generic

text: SSH library enriched for G. mexicana sequences; SSH was performed using the PCR-Select cDNA subtraction kit of Clontech with a 1000 fold excess of driver. PCR amplification products were cloned into pGEM-T easy vector of Promega

text: Second stage infective juveniles (J2)

text: Globodera mexicana

text: Santa Ana

characteristic: strain

text: GML

text: plasmid

text: pGEM-T


text: INRA

characteristic: INSDC center name

text: live

text: Generic

text: SSH library enriched for G. pallida sequences; SSH was performed using the PCR-Select cDNA subtraction kit of Clontech using a 1000 fold excess of driver. PCR amplification products were cloned into pGEM-T easy vector of Promega

text: Second stage infective juveniles (J2)

text: Globodera pallida

text: Guiclan

text: GPL

text: plasmid

text: pGEM-T


text: University of Edinburgh

text: live

text: Generic

text: The library was prepared in Murray Selkirk's lab, Imperial College, London.

text: adult

text: Nippostrongylus brasiliensis

text: Nippostrongylus brasiliensis uni-zap adult library

text: phagemid

text: pBluescript SK+


text: UFRJ - CCS - Instituto de Biologia - Departamento de Genetica

text: live

text: Generic

text: IPA 206

characteristic: cultivar

text: cDNA-AFLP fragments induced after heat stress treatment in cowpea nodule

text: Twenty days after inoculation with Bradyrhizobium spp

text: Nodule

characteristic: organ

text: Vigna unguiculata

text: Nodule

text: Vigna unguiculata nodule cDNA-AFLP fragments


text: Harvard Medical School

characteristic: INSDC first public

text: live

text: Generic

text: mRNA was purified from zebrafish adult hearts. cDNA was synthesized using a XhoI-Oligo dT adaptor-primer. EcoRI adaptors were ligated, followed by digestion with XhoI, for directional cloning into pre-digested lambda ZAP Express vector.

text: adult

text: heart

text: Danio rerio

text: EcoRI

text: XhoI

text: Zebrafish Adult hearts cDNA library

text: Lambda ZAP Express


text: Miyazaki University

text: live

text: Generic

text: common name:common carp ; treated with cortisol

text: Cyprinus carpio

text: head kidney

characteristic: tissue-type

text: Cyprinus carpio head kidney treated with cortisol


text: Karolinska Institutet

characteristic: INSDC last update

text: live

text: Generic

text: adult

text: Rattus norvegicus

text: Sprague-Dawley

text: liver

text: SSH liver cDNA library


text: live

characteristic: INSDC status

text: Generic

text: The library was supplied by Dr. Dante Zarlenga of the USDA Immunology and Disease Resistance Lab in Beltsville, MD. The cDNAs were made from 21 day L4 proximal (isolated from jejunum) and directionally cloned into the NotI/SalI sites of pSPORT1.

text: Ascaris suum

text: NotI

text: SalI

text: Ascaris suum L4 pSPORT1 Zarlenga v1

text: plasmid (ampicillin resistant)

text: pSPORT1 (Life Technologies)


text: live

text: Generic

characteristic: NCBI submission model

text: The library was constructed by Claire Murphy, Brandi Chiapelli, and Dr. James McCarter at Washington University, St. Louis. Oligo(dT)-SL1 PCR based library. Ascaris suum female gonad GZ cDNA PCR products of size >400 nucleotides containing SL1 on the 5' end and oligo(dT) on the 3' end were non-directionally cloned into pCRII-TOPO(Invitrogen) following the Topo TA cloning protocol. Dissected nematode tissues were provided by Dr. Alan Scott (ascott@jhsph.edu) of the School of Public Hygiene and Public Health at John Hopkins University in Baltimore, MD.

text: Adult

text: Ascaris suum

text: EcoRI

text: EcoRI

text: Female

characteristic: sex

text: Dissected female gonad (GZ=germinal zone, most distal region of gonad)

text: Ascaris suum female gonad GZ SL1 TOPO v1 Murphy Chiapelli McCarter

text: plasmid (kanamycin and ampicillin resistant)

text: pCRII-TOPO (Invitrogen)


text: Washington University School of Medicine

text: live

text: Generic

text: The library was constructed by Claire Murphy, Brandi Chiapelli, and Dr. James McCarter at Washington University, St. Louis. Oligo(dT)-SL1 PCR based library. Ascaris suum male intestine cDNA PCR products of size >400 nucleotides containing SL1 on the 5' end and oligo(dT) on the 3' end were non-directionally cloned into pCRII-TOPO(Invitrogen) following the Topo TA cloning protocol. Dissected nematode tissues were provided by Dr. Alan Scott (ascott@jhsph.edu) of the School of Public Hygiene and Public Health at John Hopkins University in Baltimore, MD

text: Adult

characteristic: dev-stage

text: Ascaris suum

text: EcoRI

text: EcoRI

text: Male

text: Intestine

text: Ascaris suum male gut SL1 TOPO v1 Murphy Chiapelli McCarter

text: pCRII-TOPO (Invitrogen)

characteristic: vector


text: live

text: Generic

characteristic: NCBI submission package

text: The library was constructed by Claire Murphy, Brandi Chiapelli, and Dr. James McCarter at Washington University, St. Louis. Oligo(dT)-SL1 PCR based library. Ascaris suum female head cDNA PCR products of size >400 nucleotides containing SL1 on the 5' end and oligo(dT) on the 3' end were non-directionally cloned into pCRII-TOPO(Invitrogen) following the Topo TA cloning protocol. Dissected nematode tissues were provided by Dr. Alan Scott (ascott@jhsph.edu) of the School of Public Hygiene and Public Health at John Hopkins University in Baltimore, MD

text: Adult

text: Ascaris suum

characteristic: organism

text: EcoRI

text: EcoRI

text: Female

text: Head

text: Ascaris suum female head SL1 TOPO v1 Murphy Chiapelli McCarter

text: pCRII-TOPO (Invitrogen)


text: Henna Normal Univ

text: live

text: Generic

text: Lambda Zap Express Library

characteristic: description

text: embryonic day 17 post-fertilization

text: Rattus norvegicus

text: male

text: Sprague-Dawley

text: liver

text: Rat embryonic day 17 post-fertilization

characteristic: title


characteristic: External Id

text: Tropical Medicine Department of the First Military Medical University

text: live

text: Generic

text: the SSH(suppression subtractive hybridization) products are purified and then inserted into T/A vector

text: two month old adult

text: liver

text: Rattus norvegicus

text: ApaI

text: SpeI

text: male

text: Sprague-Dawley

text: liver

text: Rat liver subtractive library

text: Plasmid

text: pGEM-T vector of Promega


text: University of Fukui

text: live

text: Generic

text: brain

text: Mus musculus

characteristic: strain

text: Mouse brain differential display PCR analysis

text: plasmid

text: pCR2.1 (Invitrogen)


text: Tamil Nadu Agricultural University

characteristic: INSDC center name

text: live

text: Generic

text: Nootripathu

text: Differentially expressing mRNAs were identified by differential display of total RNA isolated from the roots of normal and water stressed (70% leaf relative water content) plants. The upregulation of the ESTs during drought was confirmed by northern hybridization analyses.

text: Vegetative

text: Oryza sativa

text: Root tissue

text: Oryza sativa Nootripathu Differential Display


text: The Institute for Genomic Research

text: live

text: Generic

text: Salivary glands were dissected on day five after initiation of feeding. Total RNA was prepared using acid guanidium thiocyanate-phenol-chloroform extraction. The cDNA library was custom prepared by Invitrogen Corporation. Briefly, first strand cDNA was primed using oligo(dT) containing a NotI site. Size fractionated double stranded cDNA was ligated to EcoRV-NotI cleaved vector and electroporated into E.coli.

text: Adult

text: E.coli strain DH10B-TonA

characteristic: lab-host

text: Amblyomma variegatum

text: Salivary glands

text: AvSG


text: Tamil Nadu Agricultural University

text: live

text: Generic

text: Nootripathu

characteristic: cultivar

text: The differentially expressed sequence tags were identified by differential display of RNA isolated from the radicles of control and drought subjected seedlings of the drought tolerant indica rice variety, Nootripathu

text: Early seedling stage

text: Oryza sativa

text: Radicle

text: Oryza sativa Early seedling stage Differential Display


text: Beijing Institute of Heart Lung and Blood Vessel Disease

characteristic: INSDC first public

text: live

text: Generic

text: lymphocytes

characteristic: cell type

text: Rattus norvegicus

text: Rat lymphocyte


text: University of Nevada

characteristic: INSDC last update

text: live

text: Generic

text: six-week-old

text: Mesembryanthemum crystallinum

text: EcoRI

text: XhoI

text: leaf

characteristic: tissue-type

text: Ice plant Lambda Uni-Zap XR expression library, 48 hours NaCl treatment prescreened for removal of highly abundant transcripts

text: Phagemid

text: Lambda Uni-Zap XR, Bluescript SK-


text: University of Nevada

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: six-week-old

text: Mesembryanthemum crystallinum

text: EcoRI

text: XhoI

text: leaf

text: Ice plant Lambda Uni-Zap XR expression library, 0 hours NaCl treatment prescreened for removal of highly abundant transcripts

text: Phagemid

text: Lambda Uni-Zap XR, Bluescript SK-


text: Zhongshan University

text: live

text: Generic

text: adult

characteristic: dev-stage

text: Hydrophis hardwickii

text: venom gland

text: Lapemis hardwickii venom gland adult


text: live

text: Generic

text: The library was constructed by Claire Murphy, Brandi Chiapelli, and Dr. James McCarter at Washington University, St. Louis. Oligo(dT)-SL1 PCR based library. Ascaris suum male head cDNA PCR products of size >400 nucleotides containing SL1 on the 5' end and oligo(dT) on the 3' end were non-directionally cloned into pCRII-TOPO(Invitrogen) following the Topo TA cloning protocol. Dissected nematode tissues were provided by Dr. Alan Scott (ascott@jhsph.edu) of the School of Public Hygiene and Public Health at John Hopkins University in Baltimore, MD.

text: Adult

text: Ascaris suum

text: EcoRI

text: EcoRI

text: Male

characteristic: sex

text: Head

text: Ascaris suum male head SL1 TOPO v1 Murphy Chiapelli McCarter

text: plasmid (kanamycin and ampicillin resistant)

text: pCRII-TOPO (Invitrogen)

characteristic: vector


text: Washington University School of Medicine

text: live

text: Generic

characteristic: NCBI submission package

text: The library was constructed by Claire Murphy, Brandi Chiapelli, and Dr. James McCarter at Washington University, St. Louis. Oligo(dT)-SL1 PCR based library. Pristionchus pacificus mixed stage cDNA PCR products of size >400 nucleotides containing SL1 on the 5' end and oligo(dT) on the 3' end were non-directionally cloned into pCRII-TOPO(Invitrogen) following the Topo TA cloning protocol.

text: Mixed stage

text: Pristionchus pacificus

characteristic: organism

text: EcoRI

text: EcoRI

text: Pristionchus pacificus mixed stage SL1 TOPO v1 Murphy Chiapelli McCarter

text: pCRII-TOPO (Invitrogen)


text: Indiana University

text: live

text: Generic

text: oligo dt priming from poly A+ RNA, not directionally cloned

characteristic: description

text: Adult

text: E. coli

text: Heliocidaris erythrogramma

text: Radial nerve

text: Sea urchin radial nerve Lambda Zap cDNA library

characteristic: title

text: Phage

text: Bluescript SK


characteristic: External Id

text: The University of Georgia, Department of Plant Biology

text: live

text: Generic

text: Four-week-old sorghum seedlings were sprayed with spore suspension prepared from 3-week-old FRM42I, a sorghum isolate of the anthracnose pathogen Colletotrichum graminicola. Inoculated plants were kept in a 25 C dark growth chamber with 100% relative humidity for 24 hr, followed by 12/12 hr of light/dark cycle at 25 C with 90% relative humidity for another 24 hr. All leaves were harvested and quick frozen with liquid nitrogen and stored in a -80 C freezer. The library was made from poly-A RNA in the cloning vector lambda ZAP II. Clones to be sequenced were prepared by mass excision. WARNING: While most or all ESTs are expected to derive from the host plant, no effort was made to eliminate ESTs deriving from the pathogen.

text: 4-week-old seedlings infected with Colletotrichum graminicola

text: Sorghum bicolor

text: XhoI

text: EcoRI

text: Leaves

text: Pathogen-infected compatible 1 (PIC1)

text: Plasmid

text: pBluescript II SK(-) from Lambda Zap II


text: University of Insubria

characteristic: INSDC center name

text: live

text: Generic

text: Xenopus laevis

text: 5 days old whole embryos

text: cDNA from differential display on mercury-treated embryos


text: live

text: Generic

text: The library was donated for sequencing by Geert Smant from Wageningen University, Laboratory of Nematology, The Netherlands.

characteristic: lab-host

text: Globodera rostochiensis

text: BstXI

text: EcoRI

text: Globodera rostochiensis J2 pcDNAII Smant v1

text: plasmid (ampicillin resistant)

text: pcDNAII (Invitrogen)


text: University of Minnesota

text: live

text: Generic

text: Differential display of distal verses proximal outflow tract. cDNA fragments cloned into the pGEM-T Easy vector.

text: Hamburger Hamilton stage 20-27

text: Heart

characteristic: organ

text: Gallus gallus

text: Outflow tract differential display

text: pGEM-T Easy Vector


text: University of Minnesota

characteristic: INSDC first public

text: live

text: Generic

text: Differential display of Forebrain, Midbrain, Cardiacbrain (region containing the cardiac neural crest), and Full Head minus cardiac brain. cDNA fragments cloned into the pGEM-T Easy vector.

text: Hamburger Hamilton stage 6-11

text: Heart

text: Gallus gallus

text: Cardiac Neural Crest differential display I

text: pGEM-T Easy Vector


text: University of Minnesota

text: live

text: Generic

text: Differential display of Midbrain, Cardiacbrain (region containing the cardiac neural crest), and Full Head minus cardiac brain. cDNA fragments cloned into the pGEM-T Easy vector.

text: Hamburger Hamilton stage 6-11

text: Heart

text: Gallus gallus

text: Cardiac Neural Crest differential display II

text: pGEM-T Easy Vector


text: University of Minnesota

text: live

text: Generic

text: Differential display of Left verses Right Cardiacbrain (region containing the cardiac neural crest) upto the Forebrain. cDNA fragments cloned into the pGEM-T Easy vector.

text: Hamburger Hamilton stage 9-10

text: Heart

text: Gallus gallus

text: Left/Right Neural Tube differential display

text: pGEM-T Easy Vector


text: Washington University School of Medicine

text: live

text: Generic

text: The library was constructed by Claire Murphy, Brandi Chiapelli, and Dr. James McCarter at Washington University, St. Louis. Oligo(dT)-SL1 PCR based library. Parastrongyloides trichosuri free living cDNA PCR products of size >400 nucleotides containing SL1 on the 5' end and oligo(dT) on the 3' end were non-directionally cloned into pCRII-TOPO(Invitrogen) following the Topo TA cloning protocol. Nematodes were provided by Dr. Warwick Grant of AgRearch, New Zealand (warwick.grant@agresearch.co.nz). Worms were harvested from Australian brush-tailed possum (Trichosuri vulpecula) and washed thoroughly to remove host contamination. Note that despite this effort, host contamination of the library is possible.

text: Free Living

text: Parastrongyloides trichosuri

text: EcoRI

text: EcoRI

text: Parastrongyloides trichosuri FL SL1 TOPO v1 Murphy Chiapelli McCarter

text: plasmid (kanamycin and ampicillin resistant)

text: pCRII-TOPO (Invitrogen)


text: Institute of Cell and Miolecular Biology

text: live

text: Generic

text: These sequences are from 3 and 5' RACE products experiments. RNA was extracted from plant meristems.

text: Meristems

text: Streptocarpus dunnii

text: ESTs from Streptocarpus dunnii

text: where cloned, P-GEMT


text: live

text: Generic

text: Meristems

text: Streptocarpus rexii

text: ESTs from Streptocarpus rexii

text: where cloned, P-GEMT


text: live

text: Generic

text: Meristems

text: Streptocarpus saxorum

text: ESTs from Streptocarpus saxorum

text: where cloned, P-GEMT


text: Swedish University of Agricultural Sciences

text: live

text: Generic

text: Heterobasidion annosum mycelia were challenged by Pinus sylvestris seedling roots for 6 h & 72 h and the cDNA library was made from a mix of the two stages

text: Mycelia after 6 h & 72 h of challenge with pine seedling roots

text: Heterobasidion annosum

text: TC 32-1 (P)

characteristic: strain

text: Mycelia

text: Heterobasidion annosum infection stage cDNA library

text: Phage

text: Lambda