text: John Innes Centre

text: live

text: Generic

text: mesophyll cells

characteristic: cell type

text: Envy

text: cultured for 48 hours in the presence of auxin and cytokinin

text: Zinnia elegans

text: Zinnia elegans 48 hours with auxin and cytokinin


status: PUBLIC

characteristic: External Id

text: National Highland Agricultural Experiment Station

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: average insert size:1500 bp

characteristic: description

text: Solanum palustre

characteristic: organism

text: EcoRI

tag: attribute

text: XhoI

text: Solanum brevidens cDNA

characteristic: title

text: UniZap XR, pBluescript SK(+/-)

characteristic: vector


text: UMR6061, CNRS

text: live

text: Generic

text: MDCK

characteristic: cell-line

text: Canis lupus familiaris

text: Canis familiaris MDCK


text: Cold Spring Harbor Laboratory

text: live

text: Generic

text: Date: Completed 12/18/01. Submitted to CSHL 12/21/01 Library: Stratagene ZAP Express cDNA Synthesis Kit. The library was size-fractionated to enrich for large inserts. Sample: collected on the island of Hawaii, Hawaii; NYBG herbarium voucher TM2563

text: pre-anthesis; Stage 2

characteristic: dev-stage

text: flower

characteristic: organ

text: Kadua centranthoides

text: XhoI

text: Eco RI

text: Hedyotis centranthoides flower - Stage 2 (NYBG)

text: phage

text: pBK-CMV


text: Case Western Reserve University

text: live

text: Generic

text: An. stephensi midguts 24 h, 36 h and 52 h after a P. berghei infected mouse blood feed were used to make the library. The early oocyst library was made by subtracting blood-stage parasite cDNA plus midgut cDNA of mosquitoes fed a non-infected blood meal, from midgut cDNA of mosquitoes fed a P. berghei-infected blood meal.

text: Anopheles stephensi/Plasmodium berghei mixed EST library

text: early-oocyst library

text: pGEM-T-Easy


text: Case Western Reserve University

text: live

text: Generic

text: P.berghei ANKA 2.34 ; Enrichment for differentially expressed genes during parasite development by subtractive hybridization. The library represents A.stephensi and P.berghei genes expressed specifically during oocyst development (4-6 days after infectious blood meal).

text: Oocyst

text: EcoRI

text: EcoRI

text: Midgut

characteristic: tissue-type

text: Mid-oocyst subtraction library

text: Plasmid

text: pGEMT-easy


text: European Molecular Biology Laboratory

text: live

text: Generic

text: Oocyst

text: EcoRI

text: EcoRI

text: Midgut

text: Late-oocyst subtraction library

text: Plasmid

text: pGEMT-easy


text: Institute of Plant Genetics and Crop Plant Research (IPK)

text: live

text: Generic

text: Barke

characteristic: cultivar

text: average insert size is 1.6 - 1.7 kb, library was created by RZPD, Heidelberg (Dr. Bernhard Korn, Im Neuenheimer Feld 506, D-69120 Heidelberg, Germany, http://www.rzpd.de)

text: plants taken from the field at 7:30 am, sunny day, isolation of inflorescence during morning (over period of 3 hours)

characteristic: lab-host

text: Hordeum vulgare subsp. vulgare

text: SalI (5-end of cDNA)

text: NotI (3-end of cDNA)

text: total immature inflorescence (1 cm)

text: HQ


characteristic: External Id

text: live

text: Generic

text: Barke

text: seeds, 12 h and 62 h after imbibition

text: Hordeum vulgare subsp. vulgare

text: SalI (5-end of cDNA)

text: Not1 (3-end of cDNA)

text: embryo, scutellum und aleuron

text: HL


text: Shanghai University

characteristic: INSDC center name

text: live

text: Generic

text: Kluyveromyces marxianus

text: molecular neural biology library


text: University of California

characteristic: INSDC first public

text: live

text: Generic

text: Dicktoo

text: Seeds of barley (Hordeum vulgare L. cv. Dicktoo) were germinated in dishpans containing UCR-mix soil. Seedlings were kept in a growth chamber at 20C (day/night) and allowed to grow at 60-70% soil moisture content. After 3-4 days, the water was withheld in order to apply drought until the soil moisture content was reduced to 10-12%, which took another 3-4 days. At the time of extraction of epidermis, leaf water and osmotic potentials and extracted lower epidermis osmotic potentials had dropped to 15.9%, 20.2% and 24.7% of controls, respectively, as determined using a vapor pressure osmometer (Model 5100C, Wescor, Inc., Logan, UT). Epidermal strips were quickly peeled off of seedlings and immediately submerged in liquid nitrogen. About 15-20 g of epidermal tissue was collected and used to extract total RNA. Total RNA was extracted using Concert Plant RNA Reagent (Invitrogen 12322-012). Poly(A) RNA was purified using PolyATrack mRNA Isolation System IV (Promega). A cDNA library was made using a Uni-ZAP cDNA synthesis kit (Stratagene). A total of 1 million primary lambda cDNA clones were mass-excised in vivo to give a population of pBluescript SK(-) phagemids. All steps to this point were performed in the TJ Close lab at the University of California, Riverside by A. Wahid with some assistance from R.D. Fenton. Phagemids were plated on the TJC121 host strain, plasmid DNA purified, cDNA clones archived, and DNA sequences determined using the T7 primer (mainly 3' end reads) using an ABI3730 at the Arizona Genomics Institute, University of Arizona (Collura, Feuerbacher, Kim, Kudrna, Wing, Yu). Chromatogram files were transmitted to UC Riverside (by Yu), then processed at UC Riverside (by Wanamaker) using the HarvEST pipeline (http://harvest.ucr.edu) to remove vector and cloning oligo sequences and various contaminants, and to trim to a high quality region. Sequences that retained a phred 17 region of at least 100 bases were deposited to GenBank.

text: 1-2 week seedlings

text: E. coli TJC121

text: Hordeum vulgare subsp. vulgare

text: EcoRI

text: XhoI

text: lower leaf epidermis

text: Drought-stressed Dicktoo barley epidermis cDNA library

text: Phagemid pBluescript SK(-)

text: Lambda Uni-ZAP XR, excised phagemid


text: Institut of viticulture

characteristic: INSDC last update

text: live

text: Generic

text: Vitis vinifera cv. Muller-Thurgau

text: Erysiphe necator

text: germinated conidia

text: Uncinula necator germinated conidia


text: Institut of viticulture

text: live

characteristic: INSDC status

text: Generic

text: Erysiphe necator

text: germinated conidia

text: Uncinula necator infected leaf germinated conidia


text: Michigan State University

text: live

text: Generic

characteristic: NCBI submission model

text: INRA Clone 717

text: The Stem of one month-old Populus tremula x Populus alba trees was divided into sections according to the developmental stage, starting from the shoot apex until the base of the stem. The lower stem was further divided into bark and xylem by pealing of the bark. Totally we obtained six samples: three stem sections with increasing xylem production, bark, xylem and leaf with its main vein removed with a scalpel. Poly A+ RNA was extracted from those samples and subjected to cDNA-AFLP profiling. Fragments that were specifically or higher expressed in the xylem, were excised from the cDNA-AFLP gel and amplified by PCR. The PCR product was purified and sequenced directly with one of the PCR primers.

text: Stem

text: Populus tremula x Populus alba

text: Xylem

text: Hybrid aspen xylem up-regulated


text: Michigan State University

text: live

text: Generic

characteristic: NCBI submission package

text: INRA Clone 717

text: The Stem of one month-old Populus tremula x Populus alba trees was divided into sections according to the developmental stage, starting from the shoot apex until the base of the stem. The lower stem was further divided into bark and xylem by pealing of the bark. Totally we obtained six samples: three stem sections with increasing xylem production, bark, xylem and leaf with its main vein removed with a scalpel. Poly A+ RNA was extracted from those samples and subjected to cDNA-AFLP profiling. Constitutively expressed fragments or leaf up-regulated fragments were excised from the cDNA-AFLP gel and amplified by PCR. The PCR product was purified and sequenced directly with one of the PCR primers.

text: Stem

text: Populus tremula x Populus alba

text: Leaf

text: Hybrid aspen various expression patterns


text: Michigan State University

text: live

text: Generic

text: INRA Clone 717

text: The Stem of one month-old Populus tremula x Populus alba trees was divided into sections according to the developmental stage, starting from the shoot apex until the base of the stem. The lower stem was further divided into bark and xylem by pealing of the bark. Totally we obtained six samples: three stem sections with increasing xylem production, bark, xylem and leaf with its main vein removed with a scalpel. Poly A+ RNA was extracted from those samples and subjected to cDNA-AFLP profiling. Fragments that were specifically or higher expressed in the bark, were excised from the cDNA-AFLP gel and amplified by PCR. The PCR product was purified and sequenced directly with one of the PCR primers.

characteristic: description

text: Stem

text: Populus tremula x Populus alba

text: Bark

text: Hybrid aspen bark up-regulated


text: Wageningen University and Research Centre

text: live

text: Generic

text: Blue Magic

text: mRNA isolated from Iris sepals was subtracted using pedicel material as the driver. Equal amounts of mRNA from each developmental stage were pooled for cDNA synthesis. The pGEM-T Easy Vector System (Promega) was used to construct the cDNA library.

text: Iris x hollandica

characteristic: organism

text: sepals

text: subtracted Iris tepal cDNA library

text: pGEMT-Easy


text: National Institute for Agricultural Research (INRA)

text: live

text: Generic

text: A composite cDNA library was made with mRNA isolated from opposite and tension wood tissues corresponding to the mature xylem collected after debarking the stem and pealing the young differentiating xylem with a scalpel. These tissues were collected on 3 different tilted trees grown in the nursery. cDNAs were cloned in an oriented way into SfiI (A and B) restriction sites. A one-step conversion of Lambda TriplEx2 to the corresponding pTriplEx2 plasmid was done via site-specific recombination at loxP sites (Clontech, SMART cDNA library construction kit). cDNA inserts were PCR amplified using flanking primers and then sequenced on a ABI3100 Genetic Analyser (Applied Biosystem)

text: 3-years-old poplar trees grown in the nursery

text: Populus tremula x Populus alba

text: female

characteristic: sex

text: clone 717-1-B4

characteristic: strain

text: mature xylem harvested on opposite and tension wood stem

text: Poplar cDNA library from mature xylem

characteristic: title


text: live

text: Generic

text: A composite cDNA library was made with mRNA isolated from opposite and tension wood tissues corresponding to the cambial zone collected on the bark side after debarking the stem. In this respect, in addition to cambium cDNA, this library also contains very young phloem and very young xylem cDNA. The sampling was done on 3 different tilted trees grown in the nursery. cDNA were cloned in an oriented way into SfiI (A and B) restriction sites. A one-step conversion of Lambda TriplEx2 to the corresponding pTriplEx2 plasmid was done via site-specific recombination at loxP sites (Clontech ; SMART cDNA library construction kit). cDNA inserts were PCR amplified using flanking primers and then sequenced on a ABI3100 Genetic Analyser (Applied Biosystem)

characteristic: dev-stage

text: Populus tremula x Populus alba

text: female

text: clone INRA 717-1-B4

text: cambial zone harvested on the bark side

text: Poplar cDNA library from cambial zone


text: live

text: Generic

text: A composite cDNA library was made with mRNA isolated from different wood tissues corresponding to the cambial zone collected on the bark side after debarking the stem, and to the differentiating and mature xylem. The sampling was done on 2 different upright trees grown in the nursery. cDNA were cloned in an oriented way into SfiI (A and B) restriction sites. A one-step conversion of Lambda TriplEx2 to the corresponding pTriplEx2 plasmid was done via site-specific recombination at loxP sites (Clontech ; SMART cDNA library construction kit). cDNA inserts were PCR amplified using flanking primers and then sequenced on a ABI3100 Genetic Analyser (Applied Biosystem)

text: Populus tremula x Populus alba

text: female

text: clone INRA 717-1-B4

text: A pool of different stem tissues- cambial zone harvested on the bark side, differentiating xylem and mature xylem

text: Poplar cDNA library from wood tissues


text: live

text: Generic

text: A cDNA library was made with mRNA isolated from opposite wood tissues corresponding to the differentiating xylem collected with a scalpel after debarking the stem. The sampling was done on 3 different tilted trees grown in the nursery. cDNAs were cloned in an oriented way into SfiI (A and B) restriction sites. A one-step conversion of Lambda TriplEx2 to the corresponding pTriplEx2 plasmid was done via site-specific recombination at loxP sites (Clontech ; SMART cDNA library construction kit). cDNA inserts were PCR amplified using flanking primers and then sequenced on a ABI3100 Genetic Analyser (Applied Biosystem)

text: Populus tremula x Populus alba

text: female

text: clone INRA 717-1-B4

text: Young differentiating xylem harvested on opposite wood side of tilted trees

text: Poplar cDNA library from young opposite xylem


text: live

text: Generic

text: A cDNA library was made with mRNA isolated from tension wood tissues corresponding to the differentiating xylem collected with a scalpel after debarking the stem. The sampling was done on 3 different tilted trees grown in the nursery. cDNAs were cloned in an oriented way into SfiI (A and B) restriction sites. A one-step conversion of Lambda TriplEx2 to the corresponding pTriplEx2 plasmid was done via site-specific recombination at loxP sites (Clontech ; SMART cDNA library construction kit). cDNA inserts were PCR amplified using flanking primers and then sequenced on a ABI3100 Genetic Analyser (Applied Biosystem)

text: Populus tremula x Populus alba

text: female

text: clone 717-1-B4

text: Young differentiating xylem harvested on the tension wood side of tilted trees

text: Poplar cDNA library from young tension xylem


text: National Horticultural Research Institute, RDA

text: live

text: Generic

text: Acapulco

text: floral stage 9 - 14 according to Arabidopsis developmental stage

text: XL1-Blue MRF' strain

text: anther and tepal

characteristic: organ

text: Lilium hybrid division VII

text: EcoR I

text: Xho I

text: Lily anther Uni-ZAPII cDNA library

text: pBluescript SK(+)V_TYPE: phagemid

characteristic: vector


text: BioPia

text: live

text: Generic

text: branch

text: Eleutherococcus sessiliflorus

text: Eco RI

text: Spe I

text: Acanthopanax sessiliflorus cDNA library

text: pGEM-T Easy Vector


text: Institute of Plant Genetics and Crop Plant Research (IPK)

text: live

text: Generic

text: Barke

characteristic: cultivar

text: The RNA samples were pooled and subtracted against roughly 20,000 cDNA clones representing a unigene set based on sequencing results from the 22 IPK H* libraries. The insert can be relesed by EcoRI/SmaI or EcoRI/NotI. The library was custom made by the company GATC, Konstanz, Germany. Pooled tissue: culm basis incl. root initials, generative apex 5 mm, generative apex 1 cm, adventitious shoot buds < 5 mm, leaf midribs afternoon / high light / 16:00, leaf midribs / darkness / 23:30, germinating seed 12 h after imbibition, germinating seed 62 h after imbibition, embryo 8 - 12 dpa, embryo 14 - 18 dpa, embryo 20 - 26 dpa, gynoeceum, anthers, second leaf of iron starved plants (15 days old seedlings), root tips of iron starved plants (15 days old seedlings).

text: Hordeum vulgare subsp. vulgare

text: EcoRI (5-end of cDNA)

text: SmaI (3-end of cDNA)

text: pooled tissue

characteristic: tissue-type

text: BP

text: pBluescript SK+


characteristic: External Id

text: *RZPD Ressourcenzentrum fuer Genomforschung GmbH, **Institute of Plant Genetics and Crop Plant Research Gatersleben

text: live

text: Generic

text: Barke

text: 0-10 DAF (days after flowering), cDNA synthesis using pBluescript II XR cDNA-library construction kit (Stratagen) with an oligo(dT)-primer containing NotI restriction site and a SalI adapter (Invitrogen). The main library of 21500 clones was rearrayed into the sublibrary BES 1824 containing 4100 putative expression clones. Note: Due to a cloning artefact caused by the kit, in most cases the SalI site is NOT present, as well as the SalI Adapter used for cloning. To excise the insert, restriction sites upstream SalI should be used (e.g. BamHI). Average insert size is 1 kb. Library generation and sequencing was granted in context of GABI; data are also accessible at https://gabi.rzpd.de

text: 0-10 DAF (days after flowering)

text: E. coli, SCS-1/pSE111

characteristic: lab-host

text: Hordeum vulgare subsp. vulgare

text: SalI

text: NotI

text: embryosac

text: pQE30NST (AF074376)


text: The Institute for Genomic Research

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: A normalized cDNA library was custom built by Express Genomics. Oligo-dT primed cDNA was directionally cloned into NotI-EcoRV site of pExpress 1. Universal M13 primers were used to generate 5' and 3' EST data.

text: Adult and larvae

text: E. coli strain DH10B-TonA

text: Whole ticks and dissected organs

text: Rhipicephalus microplus

text: BEA

text: pExpress 1


text: University of Helsinki

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Terra Regina

text: stages 4-9

text: Gerbera hybrid cultivar

text: petal

text: Gerbera hybrid cv. 'Terra Regina' petal stages 4-9


text: University of Helsinki

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Terra Regina

text: Gerbera hybrid cultivar

text: Botrytis infected whole flower

text: Gerbera hybrid cv. 'Terra Regina' Botrytis infected whole flower


text: Advanced Center for Genome Technology, University of Oklahoma

text: live

text: Generic

text: BD/Clontech PCR-select cDNA subtraction library

characteristic: description

text: E.coli

text: Lolium arundinaceum

characteristic: organism

text: EcoR I

text: EcoR I

text: shoot

text: Tall Fescue PI283316 39 deg C Heat Stress SSH cDNA

text: Plasmid

text: pCR 2.1-TOPO


text: live

text: Generic

text: E.coli

text: Lolium arundinaceum

text: EcoR I

text: EcoR I

text: shoot

text: Tall Fescue PI283316 42 deg C Heat Stress SSH cDNA

text: Plasmid

text: pCR 2.1-TOPO


text: live

text: Generic

text: E.coli

text: Lolium arundinaceum

text: EcoR I

text: EcoR I

text: shoot

text: Tall Fescue PI283316 44 deg C Heat Stress SSH cDNA

text: Plasmid

text: pCR 2.1-TOPO


text: live

text: Generic

text: E.coli

text: Lolium arundinaceum

text: EcoR I

text: EcoR I

text: shoot

text: Tall Fescue PI297901 39 deg C Heat Stress SSH cDNA

text: Plasmid

text: pCR 2.1-TOPO


text: live

text: Generic

text: E.coli

text: Lolium arundinaceum

text: EcoR I

text: EcoR I

text: shoot

text: Tall Fescue PI297901 42 deg C Heat Stress SSH cDNA

text: Plasmid

text: pCR 2.1-TOPO


text: live

text: Generic

text: E.coli

text: Lolium arundinaceum

text: EcoR I

text: EcoR I

text: shoot

text: Tall Fescue PI297901 44 deg C Heat Stress SSH cDNA

text: Plasmid

text: pCR 2.1-TOPO


text: Cold Spring Harbor Laboratory

text: live

text: Generic

text: Library constructed using pBluescript XR kit from Stratagene. Cloned cDNA was size selected between 1-3 kb. Mark Haskins VMD, PhD, Pathology and Medical Genetics, School of Veterinary Medicine, University of Pennsylvania, 3800 Spruce Street, Philadelphia, PA 19104-6051

text: 3 month old normal canine

text: XL10 Gold

text: Brain

text: Canis lupus familiaris

text: EcoRI

text: XhoI

text: Cerebellum

text: Brain - Cerebellum Library (DOGEST8)

text: plasmid

text: pBluescript II SK


text: Cold Spring Harbor Laboratory

text: live

text: Generic

text: 3 month old normal canine

text: XL10 Gold

text: Heart

text: Canis lupus familiaris

text: EcoRI

text: XhoI

text: Whole Heart Library (DOGEST5)

text: plasmid

text: pBluescript II SK


text: live

text: Generic

text: 3 month old normal canine

text: XL10 Gold

text: Heart

text: Canis lupus familiaris

text: EcoRI

text: XhoI

text: Right Ventricle

text: Right Cardiac Ventricle Library (DOGEST6)

text: plasmid

text: pBluescript II SK


text: live

text: Generic

text: 3 month old normal canine

text: XL10 Gold

text: Heart

text: Canis lupus familiaris

text: EcoRI

text: XhoI

text: Left Ventricle

text: Left Cardiac Ventricle Library (DOGEST7)

text: plasmid

text: pBluescript II SK


text: USDA-ARS

text: live

text: Generic

text: Green Gametophyte

text: Syntrichia ruralis

text: SalI

text: NotI

text: Gametophyte rehydration Library

text: Plasmid


text: University of Minnesota

text: live

text: Generic

text: University of Minnesota Canine Brain EST Project. Tissue was taken from the frontal, occipital, temporal and parietal lobes, olfactory bulb, hippocampus, cerebellum, thalamus, hypothalamus, midbrain, pons, and medulla.

text: Adult

text: Brain

text: Canis lupus familiaris

text: NotI

text: SalI

text: Canine Brain cDNA library

text: Plasmid

text: pSPORT1 (Gibco BRL)


text: University of Delaware Graduate College of Marine Studies

text: live

text: Generic

text: toxic zoospore

characteristic: cell type

text: A serial analysis of gene expression (SAGE) library was constructed from toxic Pfiesteria shumwayae zoospores. RNA was reverse transcribed with an oligo dT - Heel primer where the Heel is a 20 base extension at the 5' end of the primer. The 3' end cDNA sequences for tags of interest were determined by amplification of cDNA using SAGE tag-specific forward primers and the Heel reverse primer. PCR products were then cloned into pCR4 TOPO-TA cloning vector and individual clones were sequenced.

text: Pfiesteria shumwayae

text: Pfiesteria shumwayae EST sequences from SAGE tags

text: pCR4 TOPO-TA


text: Microbial Ecology, Institution of Ecology, Lund University

text: live

text: Generic

text: This EST clone is originating from one of four cDNA libraries. These libraries were constructed for transcript profiling of nematophagous ascomycete Monacrosporium haptotylum in interaction with Caenorhabditis elegans. The library 'MYCELIUM' is constructed from vegetative mycelium in a saprophytic stage, the second library 'KNOB' is constructed from the separated infection structure and finally the '4h-POST INFECTION' and the '24h-POST INFECTION' libraries represent early (4h) and late (24h) post-infection stages. These libraries where analayzed in parallel and 1729 (MYCELIUM), 1450 (KNOB), 1195 (4h-POST INFECTION) and 2741 (24h-POST INFECTION) high-quality (PHRED 20) ESTs of >99bp have been deposited. The cDNA libraries were constructed from total RNA using the SMART cDNA library Construction kit (#K1051-1, Clontech, Palo Alto, CA, USA) according to the manufacturer's instructions. Full-length cDNAs were trimmed by SfiI, fractionated and directionally ligated into (lambda)pTriplEx2 arms. The lambda library was converted to a plasmid library via site-specific recombination at loxP sites in a Cre+ strain (E. coli XL1-Blue). Plasmid clones were randomly collected and analysed by DNA sequencing using a plasmid-specific forward primer (P104).

text: 4h infection

text: Escherichia coli XL1-Blue

text: Caenorhabditis elegans/Monacrosporium haptotylum mixed EST library

text: SfiI

text: SfiI

text: CBS 200.50/N2

characteristic: strain

text: Fungal and nematode tissue after 4h of infection

text: 4h-POST INFECTION

characteristic: title

text: Plasmid


text: live

text: Generic

text: 24h infection

characteristic: dev-stage

text: Escherichia coli XL1-Blue

text: SfiI

text: SfiI

text: CBS 200.50/N2

text: Fungal and nematode tissue after 24h of infection

text: 24h-POST INFECTION

text: Plasmid


text: Agriculture and Agri-food Canada

text: live

text: Generic

text: Mycelia grown on carrot agar at 20oC until confluent; perithecia induced with Tween 40 solution (25% v/v). Fruiting bodies were collected 20 days after induction. Total RNA was extracted using Trizol. cDNAs were amplified using Invitrogen GeneRacer kit. cDNA was not fractionated and was bidirectionally cloned.

text: Sexual

text: E. coli DH10B

text: Fusarium graminearum

text: EcoRI

text: DAOM 180378

text: Fg06_AAFC_ECORC_Fusarium_graminearum_perithecia

text: Plasmid

text: pGem-T easy

characteristic: vector


text: Agriculture and Agri-food Canada

text: live

text: Generic

text: This cDNA library was produced with Thermoscript RT at 50-60oC, selected for full-length by the CAP trapper method and directionally subcloned into pHSX-C.

text: Asexual

text: E. coli DH5FT

text: Fusarium graminearum

text: Sfi I

text: DAOM 180378

text: Mycelia grown in the mycotoxin-induction media GYEP for 12 days, with intermittent shaking of the liquid culture

text: Fg11_AAFC_ECORC_Fusarium_graminearum_mycelium_full-length_cDNA

text: Plasmid

text: pHSX-C


text: Agriculture and Agri-food Canada

text: live

text: Generic

text: This cDNA library was produced with Superscript II RT at 45oC in the presence of T4 gene 32 protein, selected for full-length by the CAP trapper method and directionally subcloned into pHSX-C.

text: Asexual

text: E. coli DH5FT

text: Fusarium graminearum

text: Sfi I

text: DAOM 180378

text: Fg12_AAFC_ECORC_Fusarium_graminearum_mycelium_full-length_cDNA

text: Plasmid

text: pHSX-C


text: Agriculture and Agri-food Canada

text: live

text: Generic

text: This cDNA library was produced with Superscript II RT at 45oC in the presence of T4 gene 32 protein, and is the non-full-length proportion remaining after removing the full-length proportion selected by the CAP trapper method. The library was subcloned directionally into pHSX-Ci.

text: Asexual

text: E. coli DH5FT

text: Fusarium graminearum

text: Sfi I

text: DAOM 180378

text: Fg13_AAFC_ECORC_Fusarium_graminearum_mycelium_partial_cDNA

text: Plasmid

text: pHSX-Ci


text: Agriculture and Agri-food Canada

text: live

text: Generic

text: This cDNA library was produced with Superscript III RT at 45oC in the presence of T4 gene 32 protein, and is the full-length proportion selected by the CAP trapper method. The library is subcloned directionally into pHSX-Ci.

text: Asexual

text: E. coli DH5FT

text: Fusarium graminearum

text: Sfi I

text: DAOM 180378

text: Fg14_AAFC_ECORC_Fusarium_graminearum_mycelium_full-length_cDNA

text: Plasmid

text: pHSX-Ci


text: LION bioscience AG

text: live

text: Generic

text: adult

text: pancreas

characteristic: organ

text: Canis lupus familiaris

text: Beagle

text: pancreas

text: plasmid

text: Dog pBluescript LION


text: LION bioscience AG

text: live

text: Generic

text: adult

text: brain

text: Canis lupus familiaris

text: Beagle

text: brain

characteristic: tissue-type

text: plasmid

text: Dog pBluescript LION


characteristic: External Id

text: LION bioscience AG

text: live

text: Generic

text: adult

text: testis

text: Canis lupus familiaris

text: Beagle

text: testis

text: plasmid

text: Dog pBluescript LION


text: LION bioscience AG

text: live

text: Generic

text: adult

characteristic: lab-host

text: liver

text: Canis lupus familiaris

text: Beagle

text: liver

text: plasmid

text: Dog pBluescript LION


text: LION bioscience AG

characteristic: INSDC center name

text: live

text: Generic

text: adult

text: ovary

text: Canis lupus familiaris

text: Beagle

text: ovary

text: plasmid

text: Dog pBluescript LION


text: LION bioscience AG

characteristic: INSDC first public

text: live

text: Generic

text: adult

text: kidney

text: Canis lupus familiaris

text: Beagle

text: kidney

text: plasmid

text: Dog pBluescript LION


text: LION bioscience AG

characteristic: INSDC last update

text: live

text: Generic

text: adult

text: lung

text: Canis lupus familiaris

text: Beagle

text: lung

text: plasmid

text: Dog pBluescript LION


text: LION bioscience AG

text: live

characteristic: INSDC status

text: Generic

text: adult

text: spleen

text: Canis lupus familiaris

text: Beagle

text: spleen

text: plasmid

text: Dog pBluescript LION


text: LION bioscience AG

text: live

text: Generic

characteristic: NCBI submission model

text: adult

text: muscle

text: Canis lupus familiaris

text: Beagle

text: muscle

text: plasmid

text: Dog pBluescript LION


text: LION bioscience AG

text: live

text: Generic

characteristic: NCBI submission package

text: adult

text: heart

text: Canis lupus familiaris

text: Beagle

text: heart

text: plasmid

text: Dog pBluescript LION


text: LION bioscience AG

text: live

text: Generic

text: adult

text: blood

text: Canis lupus familiaris

text: Beagle

text: blood

text: plasmid

text: Dog pBluescript LION


text: LION bioscience AG

text: live

text: Generic

text: adult

text: uterus

text: Canis lupus familiaris

text: Beagle

text: uterus

text: plasmid

text: Dog pBluescript LION


text: LION bioscience AG

text: live

text: Generic

text: adult

text: colon

text: Canis lupus familiaris

text: Beagle

text: colon

text: plasmid

text: Dog pBluescript LION


text: LION bioscience AG

text: live

text: Generic

text: adult

text: jejunum

text: Canis lupus familiaris

text: Beagle

text: jejunum

text: plasmid

text: Dog pBluescript LION


text: LION bioscience AG

text: live

text: Generic

text: adult

text: duodenum

text: Canis lupus familiaris

text: Beagle

text: duodenum

text: plasmid

text: Dog pBluescript LION


text: LION bioscience AG

text: live

text: Generic

text: adult

text: liver

text: Canis lupus familiaris

text: Beagle

text: hepatocyte

text: plasmid

text: Dog pBluescript LION


text: LION bioscience AG

text: live

text: Generic

text: adult

text: aorta

text: Canis lupus familiaris

text: Beagle

text: aorta

text: plasmid

text: Dog pBluescript LION


text: LION bioscience AG

text: live

text: Generic

text: adult

text: liver

text: Canis lupus familiaris

text: Beagle

text: hepatocytes

text: plasmid

text: Dog pBluescript LION


text: Bose Institute

text: live

text: Generic

text: wild

characteristic: cultivar

text: The subtracted cDNA library was constructed from polyA+ RNA by using the Stratagene ZAP-cDNA synthesis kit. The cDNAs were cloned directionally in Uni-ZAP XR vector. The cDNAs were excised from Uni-ZAP XR vector in the form of pBluescript SK- phagemids.

characteristic: description

text: Mature

text: Leaf

text: Oryza coarctata

text: Eco RI

text: Xho I

text: hermaphrodite

characteristic: sex

text: Subtracted cDNA library from salt stressed Porteresia leaf

text: Phagemid

text: pBluescript SK-


text: Commonwealth Scientific and Industrial Research Organisation Division of Plant Industry.

text: live

text: Generic

text: Himalaya

text: mRNA was prepared from endosperm tissues of the barley cultivar Himalaya. cDNA was synthesised from pooled 10, 12, and 15 dpa endosperm using Not I-oligo(dT)18 primer/adapter (Pharmacia Biotech), and then ligated to the Sal I-Not I site of _ZipLox vector (Life Technology) after adding a Sal I-Xho I adapter (Stratagene). Constructed by Shahjahan Ali and Bill Taylor.

text: developing endosperm tissue 10, 12, 15 dpa (days post anthesis)

text: DH10B (Life Technology)

text: Hordeum vulgare subsp. vulgare

characteristic: organism

text: Sal I

text: Not I

text: endosperm

text: Barley EST endosperm library

text: Phage


text: National Institute of Health

text: live

text: Generic

text: MDCK II

characteristic: cell-line

text: Canis lupus familiaris

text: Canis familiaris MDCK II

characteristic: title


text: Tel Aviv University

text: live

text: Generic

text: The cDNA library was made by using Botrytis cinerea mycelium grown 24h under light, in presence of 10mM methionine. The cDNA were cloned directionally in the lambda Screen-1 vector (Novagen Inc.)

text: 24h liquid growth

characteristic: dev-stage

text: Botrytis cinerea

characteristic: strain

text: 24h MET cDNA library

text: Phage

text: lambda Screen-1

characteristic: vector


characteristic: External Id

text: Tel Aviv University

text: live

text: Generic

text: The cDNA library is the result of suppression subtractive hybridization (SSH) between mRNA from mycelium grown 24h and exposed to 2000ppm ethylene for 3.5h and mRNA from mycelium grown 24h non exposed to ethylene.

text: 24h liquid growth

text: Botrytis cinerea

text: 24h ethylene SSH cDNA library

text: plasmid


text: IBP-CNRS, University of Paris XI

text: live

text: Generic

text: IGRI

text: RT-PCR were performed on shoot apex RNA. Resulting amplification product was cloned in a pGEM-T easy vector (Promega) and SP6/T7 sequenced.

text: 3-5 leaves plantlets

text: shoot apex

characteristic: organ

text: Hordeum vulgare subsp. vulgare

text: hermaphrodite

text: shoot apex

characteristic: tissue-type

text: PCR amplification from tel cDNA (barley)

text: pGEM-T easy vector (Promega)


text: The Natural History Museum

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: Phosphuga atrata

text: Whole

text: Silpha atrata cDNA

text: pCRII


text: Tokyo University of Technology

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: 6h-28d 500 mM NaCl treated leaves

text: 4 months-old

text: Bruguiera gymnorhiza

text: leaf

text: Bg01 Burma mangrove cDNA library


text: Tokyo University of Technology

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: 1h-3d 500 mM NaCl treated leaves

text: 5 months-old

text: Bruguiera gymnorhiza

text: leaf

text: Bg02 Burma mangrove cDNA library


text: Tokyo University of Technology

text: live

text: Generic

text: 2d 400 mM NaCl treated leaves

characteristic: description

text: 5 months-old

text: Bruguiera gymnorhiza

text: leaf

text: Bg03 Burma mangrove cDNA library


text: Tokyo University of Technology

text: live

text: Generic

text: 1h-12d 500 mM NaCl treated leaves

text: 5 months-old

text: Bruguiera gymnorhiza

text: root

text: Bg04 Burma mangrove cDNA library


text: Tokyo University of Technology

text: live

text: Generic

text: 0.5-24h IAA, ABA, gibberellin, salicylate, kinetin, jasmonate, Ethephon, mannitol and dehydration treated leaves

text: 5-10 months-old

text: Bruguiera gymnorhiza

text: leaf

text: Bg05 Burma mangrove cDNA library


text: Department of Primary Industries

text: live

text: Generic

text: SMART PCR cDNA synthesis kit (Clontech, USA). pGEM-T Easy vector system I (Promega, USA). E.coli XL10- Gold ultracompetent cells (Stratagene, USA).

text: Lolium arundinaceum

text: seedlings- 6 to 96 hours post-imbibition


text: Universidade Federal de Pernambuco

text: live

text: Generic

text: promastigote

text: Leishmania chagasi

text: Exponential growth phase Leishmania chagasi promastigotes cDNA library (LCEX02)


text: Cold Spring Harbor Laboratory

text: live

text: Generic

text: Library constructed using pBluescript XR kit from Stratagene. Cloned cDNA was size selected between 1-3 kb. Tissue supplied by Mark Haskins VMD, PhD, Pathology and Medical Genetics, School of Veterinary Medicine, University of Pennsylvania, 3800 Spruce Street, Philadelphia, PA 19104-6051

text: 3 month old normal canine

text: XL10 Gold

characteristic: lab-host

text: Heart

text: Canis lupus familiaris

text: EcoRI

text: XhoI

text: Cardiac muscle

text: Right Cardiac Ventricle (DOGEST6)

text: Plasmid

text: pBluescript II SK


text: Cold Spring Harbor Laboratory

text: live

text: Generic

text: 3 month old normal canine

text: XL10 Gold

text: Heart

text: Canis lupus familiaris

characteristic: organism

text: EcoRI

text: XhoI

text: Cardiac muscle

text: Left Cardiac Ventricle (DOGEST7)

text: Plasmid

text: pBluescript II SK


text: Nanjing Forestry University

text: live

text: Generic

text: Populus x canadensis

text: EcoRI

text: XhoI

text: leaf inoculated with Marssonia pathogen of Populus euramericana

text: Phage

text: pBK-CMV


text: Third Institute of Oceanography, State Oceanic Administration

text: live

text: Generic

text: E.Coli

text: Larimichthys crocea

text: spleen

text: spleen expressed sequence tags library

text: plasmid


text: Lund University, Center for Chemistry

text: live

text: Generic

text: Bonus

characteristic: cultivar

text: Zakhrabekova, S., C.G. Kannangara, D. von Wettstein and M. Hansson. Plant Physiol. Biochem. 40 (2002) 189-197

text: Dark grown for 7 days after planting, then induced by 5 hours of light

text: E.coli

text: Leaf

text: Hordeum vulgare subsp. vulgare

text: SphI

text: BamHI

text: hermaphrodite

characteristic: sex

text: Expressed sequences from Barley

text: Plasmid


text: University of Algarve, Centre of Marine Sciences

text: live

text: Generic

text: PolyA RNA from 2 pools of 8 fish (Control or estradiol-treated) was used to make a subtractive library using the PCR-Select cDNA Subtraction kit (Clontech). Randomly picked colonies were cloned in pGEM-T Easy vector (non-directional), Promega. Patricia Pinto, Cardiff, December 2002.

text: 400 g fish, September

characteristic: dev-stage

text: Gonads

text: Sparus aurata

text: Male

text: Sea bream estradiol-treated testis subtractive library

characteristic: title

text: pGEM-T Easy


text: Institute of Biotechnology

text: live

text: Generic

text: Terra Regina

text: Gerbera hybrid cultivar

text: late petal


text: Institute of Biotechnology

text: live

text: Generic

text: Terra Regina

text: Gerbera hybrid cultivar

text: whole flower, infected with Botrytis


text: Institute of Biotechnology

text: live

text: Generic

text: Terra Regina

text: 6, 8, 9

text: Gerbera hybrid cultivar

text: stamen


text: Institute of Biotechnology

text: live

text: Generic

text: Terra Regina

text: Gerbera hybrid cultivar

text: early petal


text: Institute of Biotechnology

text: live

text: Generic

text: Terra Regina

text: 2, 4, 6, 8

text: Gerbera hybrid cultivar

text: pappus bristles


text: Institute of Biotechnology

text: live

text: Generic

text: Terra Nero

text: 2, 4, 6, 8

text: Gerbera hybrid cultivar

text: pappus bristles


text: University of Bremen

text: live

text: Generic

text: Canis lupus familiaris

text: male

text: testis

text: Canis familiaris testis cDNA


text: live

text: Generic

text: barcode 459

text: Canis lupus familiaris

text: NotI

text: SalI

text: lymph node

characteristic: vector


characteristic: External Id

text: live

text: Generic

text: barcode 460

text: Canis lupus familiaris

text: NotI

text: SalI

text: lymph node


text: live

text: Generic

text: Canis lupus familiaris

text: SalI

text: NotI

text: heart


text: live

text: Generic

text: Canis lupus familiaris

text: SalI

text: NotI

text: Pancreas


text: live

text: Generic

text: Canis lupus familiaris

text: SalI

text: NotI

text: right atrium


text: live

text: Generic

text: Canis lupus familiaris

text: SalI

text: NotI


text: live

text: Generic

text: Canis lupus familiaris

text: SalI

text: NotI

text: Synovium


text: live

text: Generic

text: Canis lupus familiaris

text: SalI

text: NotI

text: kidney


text: live

text: Generic

text: Canis lupus familiaris

text: SalI

text: NotI

text: Skeletal Muscle


text: live

text: Generic

text: Control dog thyroid size selected

text: Canis lupus familiaris

text: SalI

text: NotI

text: beagle

characteristic: strain

text: thyroid


text: live

text: Generic

text: control dog

text: Canis lupus familiaris

text: SalI

text: NotI

text: beagle

text: thyroid

characteristic: tissue-type


text: live

text: Generic

text: Canis lupus familiaris

text: SalI

text: NotI

text: beagle

text: pancreas


text: live

text: Generic

text: Canis lupus familiaris

text: SalI

text: NotI

text: beagle

text: spleen


text: live

text: Generic

text: BHK

text: Canis lupus familiaris

text: SalI

text: NotI

text: beagle

text: right atrium


text: live

text: Generic

text: skeletal muscle

text: Canis lupus familiaris

text: SalI

text: NotI

text: beagle

text: skeletal muscle


text: live

text: Generic

text: right atrium

text: Canis lupus familiaris

text: SalI

text: NotI

text: beagle

text: heart


text: live

text: Generic

text: K9-left ventricle

text: BHK

text: Canis lupus familiaris

text: SalI

text: NotI

text: beagle

text: heart


text: live

text: Generic

text: left ventricle

text: BHK

text: Canis lupus familiaris

text: SalI

text: NotI

text: beagle

text: heart


text: live

text: Generic

text: K9-right ventricle

text: Canis lupus familiaris

text: SalI

text: NotI

text: beagle

text: heart


text: live

text: Generic

text: brain from normal dog

text: Canis lupus familiaris

text: SalI

text: NotI

text: beagle

text: brain


text: live

text: Generic

text: right ventricle

text: BHK

text: Canis lupus familiaris

text: SalI

text: NotI

text: beagle

text: heart


text: live

text: Generic

text: K9 aorta

text: Canis lupus familiaris

text: SalI

text: NotI

text: beagle

text: aorta


text: live

text: Generic

text: K9 salivary gland

text: Canis lupus familiaris

text: SalI

text: NotI

text: beagle

text: salivary gland


text: live

text: Generic

text: k9 mesenteric lymphnode

text: Canis lupus familiaris

text: SalI

text: NotI

text: beagle

text: Mesenteric Lymphnode


text: live

text: Generic

text: mixed

text: Canis lupus familiaris

text: SalI

text: NotI

text: beagle

text: skeletal muscle


text: live

text: Generic

text: mixed

text: Canis lupus familiaris

text: SalI

text: NotI

text: beagle

text: left atrium


text: live

text: Generic

text: TOP 10

text: Canis lupus familiaris

text: TA site

text: TA site

text: beagle

text: cartilage


text: live

text: Generic

text: operated knee (L) vs unoperated knee (R)

text: TOP 10

text: Canis lupus familiaris

text: TA site

text: TA site

text: beagle

text: cartilage


text: live

text: Generic

text: Canis lupus familiaris

text: SalI

text: NotI

text: kidney


text: live

text: Generic

text: Canis lupus familiaris

text: SalI

text: NotI

text: synovium


text: live

text: Generic

text: normal, untreated kidney

text: Canis lupus familiaris

text: SalI

text: NotI

text: beagle

text: kidney


text: live

text: Generic

text: female

text: Canis lupus familiaris

text: SalI

text: NotI

text: beagle

text: pancreas


text: live

text: Generic

text: normal, untreated canine liver

text: Canis lupus familiaris

text: SalI

text: NotI

text: beagle

text: liver


text: live

text: Generic

text: brain - dog

text: BHK

text: Canis lupus familiaris

text: SalI

text: NotI

text: beagle

text: brain


text: live

text: Generic

text: male

text: Canis lupus familiaris

text: EcoRI

text: EcoRI

text: beagle

text: pancreas


text: live

text: Generic

text: male

text: Canis lupus familiaris

text: EcoRI

text: EcoRI

text: beagle

text: Pancrease


text: live

text: Generic

text: Canis lupus familiaris

text: SalI

text: NotI

text: beagle

text: testes


text: live

text: Generic

text: control dog brain stem

text: Canis lupus familiaris

text: SalI

text: NotI

text: beagle

text: brain stem


text: live

text: Generic

text: Control dog

text: Canis lupus familiaris

text: SalI

text: NotI

text: beagle

text: thymus


text: live

text: Generic

text: dog right atrium

text: BHK

text: Canis lupus familiaris

text: SalI

text: NotI

text: beagle

text: right atrium


text: live

text: Generic

text: BHK

text: Canis lupus familiaris

text: SalI

text: NotI

text: beagle

text: brain


text: live

text: Generic

text: LPS stimulated

text: Canis lupus familiaris

text: SalI

text: NotI

text: mononuclear


text: live

text: Generic

text: whole organ

text: Canis lupus familiaris

text: SalI

text: NotI

text: kidney


text: Gene Logic Inc.

characteristic: INSDC center name

text: live

text: Generic

text: heart, liver, kidney, testis, and brain

characteristic: organ

text: Canis lupus familiaris

text: NotI

text: SalI

text: beagle

text: GLGC-LIB0001-cf Canis familiaris Normalized Mixed Tissue cDNA Library


text: National Eye Institute

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: RNA was extracted from dog lens tissue. A directionally cloned cDNA library in the pCMVSPORT6 vector (Invitrogen) was constructed at Bioserve Biotechnology (Laurel MD) essentially following the protocols of the SuperScript Plasmid System, full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. cDNA was cloned in Not I/Sal I sites. EST analysis was performed at the NIH Intramural Sequencing Center (NISC). Analyzed data available through http://neibank.nei.nih.gov.

text: Adult

text: Eye

text: Canis lupus familiaris

text: Beagle

text: Lens

text: Dog eye lens. Unnormalized (nac)

text: Plasmid


text: National Eye Institute

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: RNA was extracted from dog cornea tissue. A directionally cloned cDNA library in the pCMVSPORT6 vector (Invitrogen) was constructed at Bioserve Biotechnology (Laurel MD) essentially following the protocols of the SuperScript Plasmid System, full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. cDNA was cloned in Not I/Sal I sites. EST analysis was performed at the NIH Intramural Sequencing Center (NISC). Analyzed data available through http://neibank.nei.nih.gov.

text: Adult

text: Eye

text: Canis lupus familiaris

text: Beagle

text: Cornea

text: Dog eye cornea. Unnormalized (nad)

text: Plasmid


text: National Eye Institute

text: live

text: Generic

characteristic: NCBI submission package

text: RNA was extracted from dog eye minus lens and cornea tissue. A directionally cloned cDNA library in the pCMVSPORT6 vector (Invitrogen) was constructed at Bioserve Biotechnology (Laurel MD) essentially following the protocols of the SuperScript Plasmid System, full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. cDNA was cloned in Not I/Sal I sites. EST analysis was performed at the NIH Intramural Sequencing Center (NISC). Analyzed data available through http://neibank.nei.nih.gov.

characteristic: description

text: Adult

text: Eye

text: Canis lupus familiaris

text: Beagle

text: Eye minus lens and cornea

text: Dog eye eye minus lens and cornea. Unnormalized (nae)

text: Plasmid


text: National Eye Institute

text: live

text: Generic

text: Adult

characteristic: lab-host

text: Eye

text: Canis lupus familiaris

characteristic: organism

text: Beagle

text: Eye minus lens and cornea

text: Dog eye minus lens and cornea. Unnormalized (or)

text: Plasmid


text: Institute of Biotechnology

text: live

text: Generic

text: Terra Regina

characteristic: cultivar

text: Gerbera hybrid cultivar

text: young inflorescences


text: Institute of Biotechnology

text: live

text: Generic

text: Terra Regina

text: Gerbera hybrid cultivar

text: leaf, transgenic line 35S-gaga2


text: Institute of Biotechnology

text: live

text: Generic

text: Terra Regina

text: Gerbera hybrid cultivar

text: flower stem


text: Johns Hopkins University

text: live

text: Generic

text: Adult tissue from eye, lung, aorta, pulmonary artery and brain; neonatal tissue from liver, spleen, thymus, lung, kidney, aorta and brain; 50% is from cardiac tissues.

text: Mixed

text: Canis lupus familiaris

text: EcoRI

text: XhoI

text: Mixed

characteristic: sex

text: Mixed

text: Canine cardiovascular system biased cDNA


text: Korea University

text: live

text: Generic

text: Karl

text: Hordeum vulgare subsp. vulgare

text: 14 DAF Kernel

text: 14 DAF Barley kernel cDNA library

characteristic: title


text: Commonwealth Scientific and Industrial Research Organisation Division of Plant Industry.

text: live

text: Generic

text: Clontech PCR-Select cDNA Subtraction kit (BD Sciences) was used to make a library of cDNA clones differentially expressed during F. graminearum infection of wheat heads. 10 infected heads were collected daily for 13 days and pooled for RNA extraction, control plants were treated like infected plants, except these were mock 'inoculated' with water only. For the subtraction process, 2 ug of PolyA+ RNA was used for each tester (infected tissue) and driver (control tissue) samples, according to the manufacturer's protocol (BD Sciences). Products from secondary PCR were cloned into the pGEM-T Easy vector system (Promega) according to manufacturer's instructions, and transformed into TOP10 cells (Invitrogen), also according to manufacturer's instruction. Constructed by Agnieszka M. Mudge.

text: From anthesis to grain fill

characteristic: dev-stage

text: E. coli TOP10

text: Heads

text: Fusarium graminearum

text: Subtracted cDNA library from Fusarium graminearum infected wheat heads (sFHB)

text: plasmid

text: pGEM-T easy

characteristic: vector


characteristic: External Id

text: University of Pennsylvania

text: live

text: Generic

text: 16 weeks old

text: DH12S E. Coli cells (Invitrogen)

text: Canis lupus familiaris

text: male

text: Beagle

characteristic: strain

text: brain

text: Canine normalized brain cDNA library

text: Plasmid

text: pGEM11Zf(+) (Promega)


text: University of Pennsylvania

characteristic: INSDC center name

text: live

text: Generic

text: 16 weeks old

text: DH12S E. Coli cells (Invitrogen)

text: Canis lupus familiaris

text: Poodle derived cross breed

text: retina

characteristic: tissue-type

text: Canine normalized prcd affected retinal cDNA library

text: Plasmid

text: pGEM11Zf(+) (Promega)


text: University of Pennsylvania

characteristic: INSDC first public

text: live

text: Generic

text: library was created by subtracting a normalized canine prcd affected retinal library against non-affected library as described by Bonaldo et al., 1996.

text: 16 weeks old

text: DH12S E. Coli cells (Invitrogen)

text: Canis lupus familiaris

text: Poodle derived cross breed

text: retina

text: Canine subtracted prcd affected retinal cDNA library

text: Plasmid

text: pGEM11Zf(+) (Promega)


text: University of Pennsylvania

characteristic: INSDC last update

text: live

text: Generic

text: library was created by subtracting a normalized canine retinal library against prcd affected retinal library as described by Bonaldo et al., 1996.

text: 16 weeks old

text: DH12S E. Coli cells (Invitrogen)

text: Canis lupus familiaris

text: male

text: Beagle

text: retina

text: Canine subtracted normal retinal cDNA library 1

text: Plasmid

text: pGEM11Zf(+) (Promega)


text: University of Pennsylvania

text: live

characteristic: INSDC status

text: Generic

text: library was created by subtracting a normalized canine retinal library against a normalized brain library as described by Bonaldo et al., 1996.

text: 16 weeks old

text: DH12S E. Coli cells (Invitrogen)

text: Canis lupus familiaris

text: male

text: Beagle

text: retina

text: Canine subtracted normal retinal cDNA library 2

text: Plasmid

text: pGEM11Zf(+) (Promega)


text: University of Pennsylvania

text: live

text: Generic

characteristic: NCBI submission model

text: 16 weeks old

text: DH12S E. Coli cells (Invitrogen)

text: Canis lupus familiaris

text: male

text: Beagle

text: retina

text: Canine normalized retinal cDNA library

text: Plasmid

text: pGEM11Zf(+) (Promega)


text: Centro de Investigacion y Estudios Avanzados del IPN (CINVESTAV-IRAPUATO)

text: live

text: Generic

characteristic: NCBI submission package

text: AAA Cavendish 'Grand Nain'

text: PCI-5 (PCI= Peel Color Index)

text: fruit

characteristic: organ

text: Musa acuminata AAA Group

text: pulp

text: Banana fruit ripening subtractive library

text: plasmid

text: pCR2.1 TOPO TA


text: National Taiwan University

text: live

text: Generic

text: synonym: Bambusa oldhamii

characteristic: description

text: Bambusa oldhamii

text: Dendrocalamopsis oldhamii library


text: UMR 5546 CNRS/Universite Paul Sabatier Surfaces cellulaires et signalisations chez les vegetaux

text: live

text: Generic

text: TE differentiating cell cultures

characteristic: cell type

text: Envy

text: LXL (Late Xylogenesis Library). SSH (suppression subtractive hybirdization) library made with tester sample comprising pooled RNA from 48, 60 and 72h of cell culture in differentition medium (supplemented with auxin NAA and cytokinin BAP) and driver sample comprising pooled RNA from 0h of culture and 48, 60 and 72h in control conditions (with NAA only, with BAP only and with no hormone)

text: Late TE differentiation proccesses (48, 60 and 72h of culture in NAA+BAP medium)

text: E.coli DH5alpha

characteristic: lab-host

text: Zinnia elegans

characteristic: organism

text: SalI

text: NdeI

text: LXL

text: Plasmid

text: pGEM-T


text: Texas A&M University

text: live

text: Generic

text: Williams

characteristic: cultivar

text: genotype: AAA

text: Musa acuminata AAA Group

text: leaf tissue treated with Mycosphaerella fijiensis extracts

text: Musa acuminata cv. Williams library

characteristic: title


text: CIRAD

text: live

text: Generic

text: cavendish

text: Subtractive cDNA libraries were constructed using the PCR-select cDNA subtraction kit (Clontech, Ozyme, Saint Quentin Yvelines, France) and according to the manufacturer instructions. cDNA used as tester or driver were synthesized from and equal mixture of peel and pulp mRNA extracted from untreated-fruit harvested 40 DAF and treated- or untreated-fruit harvested at 60 and 90 DAF.

text: Early development stage from immature green to late mature green stages

characteristic: dev-stage

text: fruit

text: Musa acuminata AAA Group

text: EcoRI

text: EcoRI

text: Peel and pulp

text: Musa acuminata Subtractive cDNA libraries

text: Plasmid

text: pGEM-T easy

characteristic: vector


characteristic: External Id

text: University of Louisiana at Lafayette

text: live

text: Generic

text: primary mycelium

text: C-type

characteristic: isolate

text: Theobroma cacao

text: Moniliophthora perniciosa

text: ATCC 64190

characteristic: strain

text: Crinipellis perniciosa ATCC 64190 primary mycelium


characteristic: INSDC first public

text: live

text: Generic

text: Phyllostachys edulis

text: BE


text: National Taiwan University

characteristic: INSDC center name

characteristic: INSDC last update

text: live

text: Generic

text: Obtained by differential display-PCR

text: shoot

text: E. coli. JM109

text: Bambusa oldhamii

text: EcoRI

text: EcoRI

text: shoot

characteristic: tissue-type

text: Bamboo shoot cDNA library

text: plasmid

text: pGEMT easy vector


text: Harbin Institute of Technology

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: First-strand cDNA was synthesized with an XhoI-oligo(dT) adapter primer. After second-strand synthesis and ligation of EcoRI adapters, cDNA was digested with XhoI, generating cDNA flanked by EcoRI sites at 5' ends and XhoI sites at the 3' ends. Purified cDNA were size-fractionated by electrophoresis and ligated into the vector of pBluescript II predigested with EcoRI and XhoI.

text: Incubation for 36h

text: Trichoderma lixii

text: EcoR I

text: Xho I

text: mycelium

text: Trichoderma harzianum cDNA library

text: Plasmid

text: pBluescript II SK+


text: Centro de Investigacion Cientifica de Yucatan. A.C.

text: live

text: Generic

text: Grand Naine

text: fruit

characteristic: organ

text: Musa acuminata AAA Group

text: EcoRI

text: EcoRI

text: hermaphrodite

characteristic: sex

text: banana fruit differential display

text: Plasmid

text: pGem-TEasy


text: live

text: Generic

text: Grand Naine

text: fruit

text: Musa acuminata AAA Group

characteristic: organism

text: EcoRI

text: EcoRI

text: hermaphrodite

text: banana fruit RT-PCR

text: Plasmid

text: pGem-TEasy


text: Agriculture and Agri-food Canada

text: live

text: Generic

text: Wheat plants (cv Roblin) were grown under a 16 h light (20oC) /8 h dark (16oC) regime until mid-anthesis. Heads at mid-anthesis were point-inoculated with Fg spores (10 µl of 1 X 105 spores/ml) at every fully developed spikelet. Water inoculation was used as an experimental control on separate plants. Inoculated plants were misted for 10- second intervals every 15 min for 2 days. Inoculated spikelets were harvested 4 days post inoculation. Total RNA was extracted using guanidinium thiocyanate followed by pelleting through CsCl2. Poly(A+)RNA was isolated following the manufacturer's instructions using MPG Streptavidin Biotinylated Oligo (dT)25 Complex (CPG, NJ). A subtraction suppression hybridization (SSH) library was generated with the PCR-Select cDNA Subtraction Kit (BD Biosciences, Missisauga, ON, Canada), using cDNA from F. graminearum-inoculated tissues as the tester, subtracted with water-inoculated cDNA as the driver. The enriched SSH PCR products were cloned into the pGEM T-easy vector (Promega) to generate the libraries Ta07 and Ta07b. After sequencing, the ESTs were blasted against the Fusarium graminearum genomic sequence at NCBI and sequences with a perfect or near perfect match to Fg were renamed with the prefix Fg_Ta07.

characteristic: description

text: asexual

text: E. coli Sure Cells

characteristic: lab-host

text: Fusarium graminearum

text: EcoRI

text: EcoRI

text: DAOM 180378

text: mycelia grown on wheat heads under misting conditions

text: Fg_Ta07b_AAFC_ECORC_Fusarium_graminearum_inculated_wheat_heads

text: Plasmid

text: pGem-T easy


text: College of Animal Sciences Zhejiang University

text: live

text: Generic

text: Larimichthys crocea

text: head kidney

text: large yellow croaker head kidney cDNA library

characteristic: title

text: Cosmid


text: University of Georgia

text: live

text: Generic

text: SOLR

text: Cenchrus ciliaris

text: apomictic pistils

text: pAPO

text: Plasmid

text: pBluescript

characteristic: vector


characteristic: External Id

text: National Institute of Genetics

text: live

text: Generic

text: unfertilized egg

characteristic: dev-stage

text: Patiria pectinifera

text: APE Asterina pectinifera unfertilized egg cDNA library


text: National Institute of Genetics

text: live

text: Generic

text: 45-hour embryo

text: Patiria pectinifera

text: APG Asterina pectinifera 45-hour embryo cDNA library


text: College of Life Science, Sun Yat-Sen University

text: live

text: Generic

text: This EST sequence dose not include two PCR primer sequences, which are also part of adaptor's sequences.PCR Primers FORWARD: 5'-AGCGTGGTCGCGGCCGAGGT-3' BACKWARD: 5'-TCGAGCGGCCGCCCGGGCAGGT-3'

text: Branchiostoma belcheri tsingtauense

characteristic: strain

text: Chinese amphioxus subtractive Express Library


text: Oregon State University

characteristic: INSDC first public

text: live

text: Generic

text: The subtraction was performed using five day old larvae collected in August of 1998 and the Clontech PCR-Select cDNA subtraction kit according to the manufacturer's instructions. Starting amounts of 1.45 ug mRNA from a 3-bowl mixture of symbiotic larvae (each with an infection rate of 78%) and 1.24 ug mRNA from a 4-bowl mixture of aposymbiotic larvae were used to construct the libraries. cDNAs were inserted into a T/A cloning vector.

text: larval day 5 post-fertilization

text: Lobactis scutaria

text: whole organism

text: Fungia scutaria larval sym subtracted library


text: UMR GenHort, INRA

characteristic: INSDC center name

text: live

text: Generic

text: bagatelle

characteristic: cultivar

text: Sample name: Apex W. Plant growth place: greenhouse. Harvesting date: 01/10/2003. experimental condition: normal.

text: vegetatif

text: Rosa lucieae

text: SfiI

text: SfiI

text: apices

text: W

text: pDNR-LIB


text: Departement de Biochimie, Universite de Montreal

characteristic: INSDC last update

text: live

text: Generic

text: Euglena longa

text: Astasia longa regular


text: live

characteristic: INSDC status

text: Generic

text: Sterkiella histriomuscorum

text: Oxytricha trifallax nondirectional cDNA library


text: Third Institute of Oceanography, State Oceanic Administration,

text: live

text: Generic

characteristic: NCBI submission model

text: adult

text: spleen

text: Larimichthys crocea

text: Female

text: Pseudosciaena crocea Smart cDNA library


text: Centro de Energia Nuclear na Agricultura, Universidade de Sao Paulo

text: live

text: Generic

characteristic: NCBI submission package

text: necrotophic stage 30 days after inoculation

text: Mycelia

text: Moniliophthora perniciosa

text: ESJOH 1

text: A SSH from in vitro culture under limiting N conditions

text: pGEM-T easy vector


text: National Institute of Allergy and Infectious Diseases

text: live

text: Generic

text: PCR-based cDNA library made with SMART cDNA library construction kit from Clontech - cDNA was BamH1 digested

text: Blood stage

text: Plasmodium falciparum 3D7

text: Blood stage Plasmodium falciparum cDNA library PfBamH1

text: Phage

text: Lambda TriplEx2


text: live

text: Generic

text: PCR-based cDNA library made with SMART cDNA library construction kit from Clontech

text: Blood stage

text: Plasmodium falciparum 3D7

text: Blood stage Plasmodium falciparum cDNA library PfSuOrig

text: Phage

text: Lambda TriplEx2


text: live

text: Generic

text: PCR-based cDNA library made with SMART cDNA library construction kit from Clontech - this EST set represents primer extension experiments of selected clones

text: Blood stage

text: Plasmodium falciparum 3D7

text: Blood stage Plasmodium falciparum cDNA library PFExtensions

text: Phage

text: Lambda TriplEx2


text: live

text: Generic

text: PCR-based cDNA library made with SMART cDNA library construction kit from Clontech - cDNA was Sau3a digested

text: Blood stage

text: Plasmodium falciparum 3D7

text: Blood stage Plasmodium falciparum cDNA library PfSau3a

text: Phage

text: Lambda TriplEx2


text: Instituto de Investigaciones Marinas (IIm), CSIC

text: live

text: Generic

text: Ruditapes decussatus

text: hemocytes

characteristic: tissue-type

text: Mix of dead bacteria infected carpet shell clam SSH library


text: J. Craig Venter Institute

text: live

text: Generic

text: Grande Naine

text: mRNA obtained from a mixed sample of fruit tissues: (a) Early fruit emergence, development and cell expansion (peel and pulp), 0 weeks after shooting and 2 weeks after shooting; (b) Starch accumulation (peel and pulp), 5 weeks after shooting; (c) Fully mature pre-climacteric green fruit (pulp); (d) Early climacteric mature fruit 24 hours post ethylene exposure (pulp); (e) Climacteric mature fruit 65 hours post ethylene exposure (pulp). Library was constructed by Incyte in E. coli DH10B and kindly provided by Syngenta.

text: Fruit of varying ages and developmental stages

text: Musa acuminata AAA Group

text: NotI

text: SalI

text: Fruit

text: ESTSYN-F

text: pSport


text: J. Craig Venter Institute

text: live

text: Generic

text: Grande Naine

text: mRNA obtained from a mixed sample of leaves from a plant grown under greenhouse conditions: (a) Immature cigar leaf; (b) First fully expanded leaf; (c) Mature fully expanded leaf. Library was constructed by Incyte in E. coli DH10B and kindly provided by Syngenta.

text: Leaf of several developmental stages

text: Musa acuminata AAA Group

text: NotI

text: SalI

text: Leaf

text: ESTSYN-L

text: pSport


text: East China University of Science and Technology

text: live

text: Generic

text: yearling

text: E. coli

text: Larimichthys crocea

text: female and male

characteristic: sex

text: liver, spleen, kidney

text: Pseudosciaena crocea SSH cDNA library

text: Plasmid

text: PGEM-T


text: University of Alberta

text: live

text: Generic

text: 3 years old

text: ElectroMAX DH10B T1 Phage-Resistant Cells

text: Brain

characteristic: organ

text: Cervus canadensis nelsoni

characteristic: organism

text: Sal I (5' end of cDNA)

text: Not I (3' end of cDNA)

text: Female

text: Brain stem

text: Elk Brainstem Full-Length cDNA Library

text: pCMV SPORT 6.0


text: ESALQ - University of Sao Paulo

text: live

text: Generic

text: This cDNA library was constructed from the phytopathogen F. graminearum isolated from spikes of brazilian wheat and grown under in vitro conditions. Mycelia from fungus was collected after growing for 8 days in PDA medium. Total RNA and mRNA were extracted according to detailed protocol presented by Sokolovsky et al. (1990) and Oligotex mRNA mini kit (Qiagen), respectively. The cDNA was constructed with the SuperScript Plasmid System for cDNA Synthesis and Cloning (Invitrogen), following manufacture's protocols. Plasmid DNA was purified using a modified alkaline lysis method. Sequencing reactions were conducted using the DYEnamic ET Terminator Cycle Sequencing Kit for ABI 3100 Genetic Analyzer (Applied Biosystem) According to the manufacturer's recommendations. Clones were sequenced by the 5' end with T7 primer. The quality and clustering of the ESTs were analyzed using the softwares Phred/Cap3.

characteristic: description

text: 8 days old

text: E. coli strain DH10B

characteristic: lab-host

text: Fusarium graminearum

text: NotI

text: SalI

text: Mycelia

text: Fusarium graminearum grown under in vitro conditions

text: Plasmid


text: Institute of Plant Genetics and Crop Plant Research (IPK)

text: live

text: Generic

text: Barke

text: Stage: culm basis incl. root initials, generative apex 5mm, generative apex 1 cm, adventitious shoot buds < 5 mm, leaf midribs / afternoon / high light / 16:00, leaf midribs / darkness / 23:30, germinating seed 12 h after imbibition, germinating seed 62 h after imbibition, embryo 8 - 12 dpa, embryo 14 - 18 dpa, embryo 20 - 26 dpa, gynoeceum, anthers, second leaf of iron starved plants (15 days old seedlings), root tips of iron starved plants (15 days old seedlings). The RNA samples were pooled and substracted against roughly 20,000 cDNA clones representing an unigene set based on sequencing results from the 22 IPK H* libraries. The insert can be released by EcoRI/NotI. The library was custom-made by the company GATC Biotech AG, Konstanz, Germany. Library generation and sequencing was granted in context of GABI. Data are also accessible via GabiPD, the GABI Primary Database.

text: mixed

text: E. coli TransforMax EC100 T1R (Epicentre, Madison)

text: Hordeum vulgare subsp. vulgare

text: EcoRI

text: Not I

text: pooled tissue sample of 15 vegetative and generative tissues

text: HDP

characteristic: title

text: pBluescript II SK(+)

characteristic: vector


characteristic: External Id

text: Life Sciences School, SOOCHOW University; Xinjiang Academy of Agricultural Sciences

text: live

text: Generic

text: These sequences obtained by SSH and the populations of gene fragments generated by subtractive hybridizations are differentially expressed in photinia.

text: Photinia serratifolia

text: Subtracted cDNA library of photinia between cold acclimation and normal ones


text: Instituto de Investigaciones Marinas (IIM), CSIC

text: live

text: Generic

text: Ruditapes decussatus

text: Gill

text: Perkinsus infected clam gill SSH forward library


text: live

text: Generic

text: Ruditapes decussatus

text: Gill

text: Perkinsus infected clam gill SSH reverse library


text: live

text: Generic

text: Ruditapes decussatus

text: Hemocytes

text: Perkinsus infected clam hemocytes SSH forward library


text: live

text: Generic

text: Ruditapes decussatus

text: Hemocytes

text: Perkinsus infected clam hemocytes SSH reverse library


text: National Institute of Agricultural Science and Technology, RDA

text: live

text: Generic

text: Cordyceps tenuipes

text: fruit body

text: Paecilomyces tenuipes full-length cDNA library


text: Colorado State University

text: live

text: Generic

text: Morex

text: Seeds were micromalted at 14oC for 48 h (steeping), 20oC for 96 h, kilning steps consisting of 55oC for 3 h, 60oC for 5 h, 65oC for 5 h, 70oC for 3 h, 75oC for 2 h, 85oC for 3 h, and 90oC for 1 h. Malted seeds were collected after 96h germination at 2oC. SSH was performed between cDNA populations from dry seed (Driver) and malted seed (Tester). Forward PCR-based subtraction was performed (Tester minus Driver) and the amplification products ligated into the pTAdvantage PCR cloning vector (BD Biosciences-Clontech, Palo Alto, CA). E. coli was transformed with this ligation reaction by electroporation.

text: malted seed (day 4 germination 96 h germination, 20oC)

characteristic: dev-stage

text: E. coli DH10B

text: Hordeum vulgare subsp. vulgare

text: seed

text: MaltedMorex

text: pTAdvantage PCR cloning vector


text: DOE Joint Genome Institute

characteristic: INSDC first public

text: live

text: Generic

text: The library was made from oligo(dT)-primed cDNA and cloned into pBluescript SK- (Stratagene, La Jolla, CA). Library constructed at the Kewalo Marine Laboratory, Pacific Biosciences Research Center (University of Hawaii) and sequencing performed at DOE Joint Genome Institute (Walnut Creek, CA).

text: mixed stage/embryo and larval

text: Capitella teleta

text: CAPF Capitella sp. I ECS-2004 whole animal, mixed stage/embryo and larval


text: DOE Joint Genome Institute

characteristic: INSDC center name

text: live

text: Generic

text: The library was made from oligo(dT)-primed cDNA and cloned into the SalI (5') and NotI (3') sites of pBluescript SK- (Stratagene, La Jolla, CA). Library constructed at Williams College (Williamstown, MA) and sequencing performed at DOE Joint Genome Institute (Walnut Creek, CA).

text: Embryos >48hr after egg deposition

text: DH10B (Ti phage resistant)

text: Capitella teleta

text: EcoRV

text: NotI

text: CAWT Capitella sp. I ECS-2004 Primary Late Library, Embryos >48hr after egg deposition

text: pExpress


text: DOE Joint Genome Institute

text: live

text: Generic

text: Embryos <36hr after egg deposition

text: DH10B (Ti phage resistant)

text: Capitella teleta

text: EcoRV

text: NotI

text: CAWU Capitella sp. I ECS-2004 Primary Early Library, Embryos <36 hr after egg deposition

text: pExpress


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT)-primed cDNA and cloned into the SalI (5') and NotI (3') sites of pBluescript SK- (Stratagene, La Jolla, CA). Library is subtracted. Library constructed at Williams College (Williamstown, MA) and sequencing performed at DOE Joint Genome Institute (Walnut Creek, CA).

text: Embryos >48hr after egg deposition

text: DH10B (Ti phage resistant)

text: Capitella teleta

text: EcoRV

text: NotI

text: CAWW Capitella sp. I ECS-2004 Subtracted Late Library, Embryos >48hr after egg deposition

text: pExpress


text: University of Alberta

text: live

text: Generic

text: Common Name: Rocky Mountain Elk

text: 3 years old

text: ElectroMAX DH10B T1 Phage-Resistant Cells

text: Pharygeal

text: Cervus canadensis nelsoni

text: Sal I (5' end of cDNA)

text: Not I (3' end of cDNA)

text: Female

text: Retro Pharyngeal

text: Elk Retro Pharyngeal Full-Length cDNA Library

text: pCMV SPORT 6.0


text: MPI for Molecular Genetics

text: live

text: Generic

text: Ruditapes decussatus


text: MPI for Molecular Genetics

text: live

text: Generic

text: Ruditapes philippinarum


text: Korea University

text: live

text: Generic

text: Seeds of Bentgrass were irradiated with ionized beam. Total RNA was extracted from leaves, roots, and stolon of irradiated Bentgrass (Agrostis palustris Huds.) under drought stress. Mixture of fresh samples (1g from each treatment) obtained from 3, 4, 5 days after treatment.

text: Agrostis stolonifera var. palustris

text: Leaf, root, stolon

text: cDNA library of Bentgrass (Agrostis palustris Huds.) under drought stress


text: University Santiago de Compostela

text: live

text: Generic

text: Total RNA was extracted from pooled tissues of Scophthalmus maximus. cDNA library was constructed with equal amounts of RNA from each tissue using the Uni-ZAP XR Vector. The cDNA library was generated as part of Scophthalmus maximus EST project.

text: liver, spleen and head kidney

text: Scophthalmus maximus

text: EcoRI

text: XhoI

text: Immune

text: S. maximus Aeromonas cDNA library

text: Uni-ZAP XR vector


text: University Santiago de Compostela

text: live

text: Generic

text: liver, spleen and head kidney

text: Scophthalmus maximus

text: EcoRI

text: XhoI

text: Immune

text: S. maximus control cDNA library

text: Uni-ZAP XR vector


text: University Santiago de Compostela

text: live

text: Generic

text: liver, spleen and head kidney

text: Scophthalmus maximus

text: EcoRI

text: XhoI

text: Immune

text: S. maximus Philasterides cDNA library

text: Uni-ZAP XR vector


text: Southeast Missouri State University

text: live

text: Generic

text: vegetative monokaryotic mycelium

text: E. coli XL10-Gold

text: Coprinopsis cinerea

text: EcoRI

text: XhoI

characteristic: strain

text: Coprinus cinereus osmotic shock mycelial cDNAs

text: phagemid

text: pBluescript II SK+


text: DOE Joint Genome Institute

characteristic: INSDC last update

text: live

text: Generic

text: In Vivo Conditions: mycelium in contact with roots of cotton and maize. The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected >2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. RNA was obtained from C. Kenerley (Texas A&M University, College Station, TX) and the library was constructed and sequenced at the DOE Joint Genome Institute (Walnut Creek, CA).

text: fungal mycelium 12-48 hrs incubation

text: DH10B T1 resistant

text: Trichoderma virens

text: SalI

text: NotI

text: asexual

text: fungal mycelium

text: CBHB Trichoderma virens strain Gv29-8 fungal mycelium


text: DOE Joint Genome Institute

text: live

characteristic: INSDC status

text: Generic

text: In Vivo Conditions: mycelium in contact with roots of cotton and maize. The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 1.2-2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. RNA was obtained from C. Kenerley (Texas A&M University, College Station, TX) and the library was constructed and sequenced at the DOE Joint Genome Institute (Walnut Creek, CA).

text: fungal mycelium 12-48 hrs incubation

text: DH10B T1 resistant

text: Trichoderma virens

text: SalI

text: NotI

text: asexual

text: fungal mycelium

text: CBHC Trichoderma virens strain Gv29-8 fungal mycelium


text: DOE Joint Genome Institute

text: live

text: Generic

characteristic: NCBI submission model

text: fungal mycelium 12-48 hrs incubation

text: DH10B T1 resistant

text: Trichoderma virens

text: SalI

text: NotI

text: asexual

text: fungal mycelium

text: CBHF Trichoderma virens strain Gv29-8 fungal mycelium


text: DOE Joint Genome Institute

text: live

text: Generic

characteristic: NCBI submission package

text: fungal mycelium 12-48 hrs incubation

text: DH10B T1 resistant

text: Trichoderma virens

text: SalI

text: NotI

text: asexual

text: fungal mycelium

text: CBHG Trichoderma virens strain Gv29-8 fungal mycelium


text: Guangxi University

text: live

text: Generic

text: Equal amounts of mature taproot tissues by weight were sampled from two ends and central sections of the roots of 210-day-old cassava plants. Root tissues sampled from three plants with the same size were pooled and ground in liquid nitrogen to isolate the total RNA. About 5 ug of the resulting total RNA were employed for isolation of mRNA. Approximately 500 ng of mRNA were used to synthesis the first-strand cDNA which was then used to synthesis double-strand cDNA. The double-Strand cDNA was cloned into the NotI and SalI sites of pBluescript SK(+).

text: 210-day-old plants grown in the experimental fields of Agricultural College, Guangxi University, China

text: Manihot esculenta

text: root

characteristic: tissue-type

text: Cassava root 210-day-old plants cDNA library

text: pBluescript SK(+)


text: Faculdade de Medicina de Ribeirao Preto da Universidade de Sao Paulo

text: live

text: Generic

text: Transcripts down-regulated in N. crassa nuc-2A mutant strain grown under Pi shortage at acid pH.

text: E. coli Mos-Blue

text: Neurospora crassa

text: Neurospora crassa SSH Library AR01

text: Plasmid

text: pGEM-T


text: The University of Tokyo

text: live

text: Generic

text: Sesbania rostrata

text: stem nodule

text: Sesbania rostrata cDNA-AFLP fragment


text: Univ of Tsukuba

text: live

text: Generic

text: Saccostrea kegaki

text: Saccostrea kegaki mRNA


text: University of Notre Dame

text: live

text: Generic

text: adult

text: Aedes aegypti

text: male

characteristic: sex

text: testes

text: T37 testes subtractive


text: University of Notre Dame

text: live

text: Generic

text: adult

text: Aedes aegypti

text: male

text: testes


text: University of Notre Dame

text: live

text: Generic

text: adult

text: Aedes aegypti

text: male

text: Red

text: testes

text: Red male


text: EA 4228 ECOMERS, University of Nice

text: live

text: Generic

text: The library was prepared from total RNA extracted from tentacules of symbiotic sea anemones. polyA+ cDNAs were size selected and directionally ligated into the pSPORT1 vector (NotI/SalI). A directional sequencing (5'-3') using M13Reverse primer was performed by Genoscope (France). WARNING: this library contains also cDNAs derived from the sea anemone's symbionts: the dinoflagellate Symbiodinium sp, temperate A clade.

text: Adult

text: Anemonia viridis

text: Symbiotic sea anemone (Anemonia viridis) cDNA library

text: Plasmid


text: University of Rochester

text: live

text: Generic

text: 0-48 hours after egg deposition: meiosis through gastrulation

text: Tritia obsoleta

text: collected near Woods Hole MA

text: Ilyanassa (snail) 0-48h

text: phagemid

text: Lambda Zap II


text: Faculdade de Ciencias Farmaceuticas, Universidade de Sao Paulo

text: live

text: Generic

text: Golden

characteristic: cultivar

text: Papaya fruit were harvested at color break to 25% yellow. Ripening was induced by exogenous ethylene: fruit were left in a 240 dm3 chamber and exposed to a concentration of 100 ppm ethylene in a constant flow-through system during 2 h, and 10 h in a closed system. After treatment, fruit were ventilated and left to ripe under controlled temperature and humidity. Fruit before ethylene treatment were taken as the sample of unripe papayas. Fruit that were at the peak of respiration and low pulp firmness were the sample of climacteric ripe papayas. mRNA Differential display was performed using a total of 90 primer pairs combinations according to the instructions on the Delta Differential Display Kit (Clontech Lab.).

text: 150 days after anthesis

text: E.coli

text: Fruit

characteristic: organ

text: Carica papaya

text: Hermaphrodite

text: Pulp

text: Induced ripening papaya pulp

text: Plasmid


text: live

text: Generic

text: 3' 454 library

characteristic: description

text: Pleurobrachia bachei

characteristic: organism

text: total animal

text: 454 library of the total animal Pleurobrachia bachei


text: live

text: Generic

text: 3' 454 library

text: Pleurobrachia bachei

text: Aboral organ

text: 3' 454 library of the Aboral organ from Pleurobrachia bachei

characteristic: title


text: live

text: Generic

text: 5' 454 library

text: Pleurobrachia bachei

text: Aboral organ

text: 5' 454 library of the Aboral organ from Pleurobrachia bachei


text: Bioengineering College, Chongqing University

text: live

text: Generic

text: The Creator SMART cDNA libarary construction kit from BD Bioscience Clontech (Palo Alto, CA), combined with TRIMMER-DIRECT cDNA Normalization kit (Evrogen, Moscow), were used to generate this cDNA library. The resulting cDNAs were cloned into pDNR-Lib vector.

text: conidium germination and appressorium differentiation

text: Metarhizium acridum

text: cDNA library of Metarhizium acridum infecting locust wings

text: pDNR-Lib

characteristic: vector


characteristic: External Id

text: Nanjing University

text: live

text: Generic

text: three leaves seedlings

text: Kosteletzkya pentacarpos

text: Kosteletzkya virginica seedlings during the stage of three leaves


text: Tottori University

text: live

text: Generic

text: Aichi-first

text: 4-week old

characteristic: dev-stage

text: Solanum lycopersicum

text: leaf

text: Solanum lycopersicum Aichi-first leaf 4-week old


text: Duke University Medical Center - Department of Neurobiology

characteristic: INSDC first public

text: live

text: Generic

text: The library was constructed as described by Carninci, P. (2003) in DNA Microarrays: A Molecular Cloning Manual, eds. Bowtell, D. & Sambrook, J. (Cold Spring Harbor, NY), pp. 647-670.

text: juvenile

text: brain

text: Taeniopygia guttata

text: EcoRI

text: BamHI

text: brain

text: library 69 - regular (Single juvenile learning) - male

text: Plasmid


text: Departement de Biochimie, Universite de Montreal

characteristic: INSDC center name

text: live

text: Generic

text: previous organism:Cercomonas longicauda

text: Paracercomonas marina

text: Paracercomonas marina Homemade, Regular (lib1_cl)


text: Max Planck Institute for Chemical Ecology

text: live

text: Generic

text: normalized cDNA library construction using the Trimmer-Direct cDNA normalization kit (www.evrogen.com) and SMART technology (www.clontech.com)

text: first instar

text: Manduca sexta

text: SfiI

text: SfiI

text: midgut

text: cDNAs of Manduca sexta regulated during interaction with its native host Nicotiana attenuata

text: plasmid

text: pUCLIB (EU918604)


text: University of Wisconsin-Milwaukee

text: live

text: Generic

text: Library constructed from RNA obtained from the livers of juvenile yellow perch (equal males and females).

text: juvenile

text: Perca flavescens

text: pooled liver

text: Yellow perch control liver library

text: pBK-CMV


text: University of Wisconsin-Milwaukee

text: live

text: Generic

text: Library constructed from RNA obtained from the livers of juvenile yellow perch (equal males and females) treated for 9 weeks with 15ug estradiol-17B/gm diet.

text: juvenile

text: Perca flavescens

text: pooled liver

text: Yellow perch estrogen-stimulated liver library

text: pBK-CMV


text: Forestry and Forest Products Research Institute

text: live

text: Generic

text: fruit body

text: Pleurotus ostreatus

characteristic: strain

text: subtraction by cDNA-RDA under simulated microgravity


text: Indian Institute of Technology, Kharagpur

characteristic: INSDC last update

text: live

text: Generic

text: JRC 321

text: Total RNA was isolated from fibre forming stage of jute stem by hot borate method and purified by isopycnic CsCl density gradient ultracentrifugation. Poly (A+) mRNA was isolated using oligo (dT) cellulose. Double-strand cDNA was synthesized using the SMARTTM cDNA Library Construction Kit (Clontech Laboratories Inc., Palo Alto, CA, USA) following the manufacturer's instruction. The double stranded cDNA synthesized was size fractionated using Sephacryl S-300. Each of the fractions was then used to generate cDNA library. The library was hybridized with double stranded cDNA probe generated from poly(A)+mRNA of non-fibre forming stage of jute stem. After three round of hybridization, unhybridized clones were selected. The selected clones were then subjected to single pass sequencing. The resultant sequences were subjected to VecScreen for removing the vector sequences. The clones were putatively identified by searching against plant database in TIGR using tblastx and tblastN. Clones with e-value greater than 10-10 and sequence similarity of more than 95% were considered significant.

text: 20-45 days after sowing

text: E.coli DH10B

characteristic: lab-host

text: Stem

text: Corchorus capsularis

text: SfiIA

text: SfiIB

text: Hermaphrodite

text: Bast fiber and associated tissues

text: Jute Plasmid library 20-45 days after sowing

text: Plasmid

text: pDNR LIB


text: Universita di Catania, Facolta di Agraria

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Tarocco (Sciara)

text: Flesh

text: Citrus sinensis

text: SacII

text: SpeI

text: Flesh

text: Citrus sinensis low-temperature inducible suppression subtractive hybridization (SSH) library

text: Plasmid

text: pGEM-T Vector


text: Sabanci University

text: live

text: Generic

characteristic: NCBI submission package

text: Kiziltan

text: Suppression-subtractive hybridization was performed using the PCR-Select cDNA Subtraction Kit (Clontech) according to the manufacturer's protocol. Approximately 100ng PCR-amplified cDNA were ligated without further purification into 50ng pGEM-T Easy vector (Promega).

text: 5 weeks after germination

text: E. coli

text: Triticum turgidum subsp. durum

text: EcoRI

text: durum

characteristic: sub species

text: Leaves

characteristic: tissue-type

text: Plasmid

text: pGemT-Easy


text: Sabanci University

text: live

text: Generic

text: Kiziltan

characteristic: cultivar

text: 5 weeks after germination

text: E. coli

text: Triticum turgidum subsp. durum

text: EcoRI

text: durum

text: Roots

text: Plasmid

text: pGemT-Easy


text: Sabanci University

text: live

text: Generic

text: TR 39477

characteristic: description

text: 5 weeks after germination

text: E. coli

text: Triticum dicoccoides

text: EcoRI

text: Leaves

text: Plasmid

text: pGemT-Easy


text: Sabanci University

text: live

text: Generic

text: TR 39477

text: 5 weeks after germination

text: E. coli

text: Triticum dicoccoides

characteristic: organism

text: EcoRI

text: Roots

text: Plasmid

text: pGemT-Easy


text: Sabanci University

text: live

text: Generic

text: 5 weeks after germination

text: E. coli

text: Triticum dicoccoides

text: EcoRI

text: Leaves

characteristic: title

text: Plasmid

text: pGemT-Easy


text: Sabanci University

text: live

text: Generic

text: 5 weeks after germination

text: E. coli

text: Triticum dicoccoides

text: EcoRI

text: Roots

text: Plasmid

text: pGemT-Easy

characteristic: vector


characteristic: External Id

text: University of Washington

text: live

text: Generic

text: Mercenaria mercenaria

text: Mercenaria mercenaria Differential Display Products GeneFishing


text: Louisiana State University

text: live

text: Generic

text: Eggs, Instar, worker, soldier, Male reproductive, Female reproductive, Nymph

characteristic: dev-stage

text: Coptotermes formosanus

text: Whole body

text: Coptotermes formosanus Normalized polyphenic library


text: The Johns Hopkins University

characteristic: INSDC first public

text: live

text: Generic

text: Karenia brevis

text: Karenia brevis Multi-strain Library


text: Hunan Normal University

characteristic: INSDC center name

text: live

text: Generic

text: mRNA were purified from total RNA isolated from the early development stage of the gonad of the gynogenetic hybrid of red crucian carp X common carp, and reversed to cDNA.

text: early development stage of gonad

text: gonad

characteristic: organ

text: Carassius auratus x Cyprinus carpio

text: female

characteristic: sex

text: gonad of hybrid fish differentially express library


text: UMR INRA/Universite Henri Poincare 1136

characteristic: INSDC last update

text: live

text: Generic

text: The experimental site is a 100 year-old oak forest with a continuous canopy and a hornbeam understory (Quercus petraea (Mattuschka) Liebl., Quercus robur Ehrh. and Carpinus betulus L.) located in the State Forest of Champenoux in north-eastern France (48o75'N, 6o35' E, altitude 250 m). One hundred ectomycorrhizal root tips were sampled using forceps, cleaned of soil, organic matter, or litter fragments, and immediately frozen in liquid nitrogen. RNA extractions were carried out using the protocol described in the paper. A full-length cDNA library of L. quietus - Q. petraea ECMs was constructed from 1 ?g of total RNA using the SMART cDNA synthesis kit in lTriplEx2 (Clontech, Palo Alto, California). The resulting cDNA was packaged into lambda phages using the Gigapack III Gold packaging kit (Stratagene, La Jolla, USA). The pTriplEx2 phagemid clones in Escherichia coli were obtained by using the mass in vivo excision protocol according to the manufacturer's instructions (Clontech). cDNA inserts bacterial clones derived from the cDNA library were amplified and inserts longer than 500 bp were single-pass sequenced from the 5' end using the FORNAT primer (5'-AAGCGCGCCATTGTGTTGGTACCC-3') using a CEQ 8000XL sequencer (Beckman-Coulter).

text: ectomycorrhizas

text: Lactarius quietus

text: oak forest ectomycorrhizas

characteristic: strain

text: Lactarius quietus-Quercus petraea ectomycorrhizas from the State forest of Champenoux


text: Sars International Centre for Marine Molecular Biology

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: mRNA from Drosophila serrata whole bodies from different developmental stages. The sample comprised larvae at the last instar and five adult stages: day 0 (emergence), day 1, day 2, day 3, and day 4. Within this sample, five populations were represented, spanning the geographical distribution of D. serrata on the east coast of Australia: Cooktown, Cardwell, Sarina, Brisbane and Forster.

text: various

text: Drosophila serrata

text: whole body

text: D. serrata mRNA from different develop. stages


text: Institute of Himalayan Bioresource Technology

text: live

text: Generic

characteristic: NCBI submission package

text: Leaf

text: Picrorhiza kurrooa

text: Leaf

text: cDNA library of Picrorhiza kurrooa Royle ex Benth leaf

text: Plasmid

text: pGEMT-Easy vector (Promega)


text: Forestry and Forest Products Research Institute

text: live

text: Generic

text: collection_date: 28-Nov-2003

text: Cryptomeria japonica

characteristic: tissue-type

text: Cryptomeria japonica xylem (sapwood-heartwood transition zone)


text: University of Victoria

text: live

text: Generic

text: Tissue contributors: Microtek Intl Inc.

text: Caligus rogercresseyi

text: whole tissue


text: University of Victoria

text: live

text: Generic

text: Tissue contributors: Microtek Intl Inc.

text: Caligus rogercresseyi

text: whole body


text: School of Life Sciences, Shandong University

text: live

text: Generic

characteristic: cultivar

text: Maize (Zea mays L.) inbred line DH4866 was used in this study. Plants grew in flowerpots containing field soil under natural conditions. When the tassel spread out of the uppermost leaf, some plants were treated with drought stress for 7 days, and others were left under normal-watered conditions as control. In the period of the stress, the middle part of the top fully expanded leaves of the plants under water stress treatment and the control were taken at 8:00 am each day, and the leaf osmotic potential was measured by the Fiske Micro-Osmometer (FISKE ASSOCIATES, USA). The stressed plants were watered every day to maintain a similar water-deficiency state by adjusting water supply, according to the leaf osmotic potential. And the leaves osmotic potential of the stressed plants was hold to -0.4 to -0.5Mpa, while the osmotic potential was -0.2 to -0.25Mpa in the leaves of the control plants. During the stress, there were visible signs of water deficiency such as leaf rolling during the daytime, though at night the rolled leaves spread out. Maize plants were stress-treated at the beginning of heading stage. After 7 days of water deficiency by withholding water, the plants began to enter the flowering stage. The maize flag leaves were harvested at 168h (7 day) of stress treatment, frozen in liquid nitrogen immediately and stored at -80oC for further analysis. Unstressed plants as controls were harvested at the same time as the stressed plants. And one sample consisted of the leaves from three independent plants under the same condition. Total RNA was isolated respectively from samples of stressed and control leaves by water-saturation phenol-guanidine isothiocyanate-chloroform method and mRNA was extracted from the RNA pool using an OligotexTM mRNA Purification Kit (QIAGEN, USA). Forward-SSH library was constructed using cDNA synthesized from treated plants as tester and that from the control plants as driver with the Clontech PCR-SelectTM cDNA subtraction Kit (CLONTECH, USA) according to the manufacturer's protocol.

text: flowering time

text: Zea mays

text: leaf

text: maize leaf suppression subtractive hybridization library


text: live

text: Generic

text: Leaf

text: Sinopodophyllum hexandrum

text: Leaf

text: Podophyllum hexandrum suppression subtractive hybridization (SSH) library

text: plasmid

text: pGEM-T Easy (Promega)


text: China Agricultural University

text: live

text: Generic

text: Triticum aestivum

text: root

text: Triticum aestivum root (China Agricultural University)


text: Austrian Research Centers GmbH - ARC

text: live

text: Generic

text: Idared

text: Total RNA was prepared from whole in-vitro plants and used as a template for amlification of cDNAs and suppression subtractive hybridization (SSH)(Clontech?). The obtained clones were spotted onto glass slides coated with g-amino- propyl-silane (GAPS II; Corning Inc., Corning, NY, USA). Arrays were co-hybridized with labelled cDNAs from control and Erwinia-infected plants and subsequently analysed. Differentially expressed clones identified were sequenced from both directions (M13 primers). Total RNA was prepared from peach bark and used as a template for cDNA synthesis using the SuperSMART system (Clontech, Palo Alto, CA). Amplification of cDNAs and suppression subtractive hybridization (SSH) followed the manufacturer's protocol for SSH (Clontech). PCR-amplified products resulting from two rounds of hybridization were cloned non-directionally into pCR2.1 (Invitrogen, Carlsbad, CA). Colony PCR using M13 forward and reverse primers was used to identify recombinant plasmids containing inserts >200 bp. Recombinant plasmid DNA was sequenced in both directions using Nested PCR primers 1 and 2R (Clontech) or M13 forward and reverse primers. The sequences obtained from the E Reverse library represent sequences downregulated at 5oC during exposure to short day (8 h light/16 h dark) photoperiods.

characteristic: description

text: E. coli TOP10 (Invitrogen)

characteristic: lab-host

text: whole plant

text: Malus domestica

text: monoecious

text: stem and leaves

text: Subtraction suppressive hybridization library ID Forward

text: TOPO-TA (Invitrogen)


text: Universidad de Chile

text: live

text: Generic

text: double stranded cDNA was digested with VspI and TaqI and adapters where selectively ligated to each end. cDNA fragments were amplified and selected by cDNA-AFLP technique.

text: Gelatoporia subvermispora

characteristic: organism

text: Mn+2 responsive transcript derived fragments

characteristic: title


characteristic: External Id

text: Shanghai Ocean University

text: live

text: Generic

text: Ctenopharyngodon idella

text: Liver, Gill, and Intestine

text: Liver, Gill and Intestine of Ctenopharyngodon idellus Library

text: Plasmid

text: pDNR-LIB

characteristic: vector


text: Kunming University of Science and Technology

characteristic: INSDC first public

text: live

text: Generic

text: received sunlight for 2, 4, 6, and 8 days, respectively

characteristic: dev-stage

text: Pyrus pyrifolia

text: Yunhong 1

text: colored pericarp

text: colored Yunnan red pear pericarp suppression subtractive hybridization cDNA library


text: Kobe University

characteristic: INSDC center name

text: live

text: Generic

text: Abu 70

text: response to infection of Striga hermonthica

text: 2 weeks old hydroponically grown

text: Sorghum bicolor

text: root

text: Striga infected roots cDNA library


text: Agricultural University of Athens

text: live

text: Generic

text: Mature versus juvenile carob tree shoot tissue SSH library (tissue obtained during summer).

text: shoot

characteristic: organ

text: Ceratonia siliqua

text: shoot tissue

text: Carob tree shoot tissue differentially expressed SSH library, Mature versus juvenile, summer


text: Agricultural University of Athens

characteristic: INSDC last update

text: live

text: Generic

text: Juvenile versus mature carob tree shoot tissue SSH library (tissue obtained during winter).

text: shoot

text: Ceratonia siliqua

text: shoot tissue

text: Carob tree shoot tissue differentially expressed SSH library, Juvenile versus mature, winter


text: Agricultural University of Athens

text: live

text: Generic

text: Juvenile versus mature carob tree shoot tissue SSH library (tissue obtained during summer).

text: shoot

text: Ceratonia siliqua

text: shoot tissue

text: Carob tree shoot tissue differentially expressed SSH library, Juvenile versus mature, summer


text: Agricultural University of Athens

text: live

text: Generic

text: Mature versus juvenile carob tree shoot tissue SSH library (tissue obtained during winter).

text: shoot

text: Ceratonia siliqua

text: shoot tissue

text: Carob tree shoot tissue differentially expressed SSH library, Mature versus juvenile, winter


text: University of Copenhagen

text: live

text: Generic

text: A mixed cDNA library from barley epidermal cells and Blumeria graminis haustoria. These sequences are determined to be from Blumeria graminis based on strong similarity to fungal sequences in the database.

characteristic: isolate

text: Hordeum vulgare cv. Golden Promise

text: Blumeria hordei

text: Blumeria graminis cDNA from mixed barley epidermal cells / Blumeria graminis haustoria library

text: pDNR-LIB (Clontech)