text: Indian Institute of Science

text: live

text: Generic

characteristic: NCBI submission model

text: Planococcus lilacinus

text: Planococcus lilacinus library (Mohan KN)


status: PUBLIC

characteristic: External Id

text: Biozentrum der Universitaet Wuerzburg

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission package

text: PSM

tag: attribute

characteristic: cell-line

text: Xiphophorus maculatus x Xiphophorus hellerii

characteristic: organism

text: melanoma

characteristic: tissue-type

text: Xiphophorus maculatus x Xiphophorus helleri PSM

characteristic: title


text: Shanghai Medical University

text: live

text: Generic

text: obtained from hypersensitive rats by differential display analysis

characteristic: description

text: adult

characteristic: dev-stage

text: Rattus norvegicus

text: thymus

text: Rattus norvegicus thymus adult


text: Boston University School of Medicine

text: live

text: Generic

text: Megakaryocyte

characteristic: cell type

text: Y10 (subclone of L8057)

text: Isolated using differential display RT-PCR

text: Adult

text: Mus musculus

text: Bone marrow

text: Thrombopoietin regulated mRNA


text: PUMC

text: live

text: Generic

text: Homo sapiens

text: testis

text: Homo sapiens testis (Wang LF)


text: VAMC

text: live

text: Generic

text: myoblast

text: Mus musculus

text: Murine myoblast library


text: Washington University School of Medicine

text: live

text: Generic

text: Williams

characteristic: cultivar

text: This cDNA library was constructed from mRNA isolated from immature cotyledons (100-200mgs) of old greenhouse grown plants. The cDNA library was prepared using the Stratagene pBluescript II SK(+) library construction kit. Complementary DNA was synthesized from mRNA using a primer consisting of a poly (dT) sequence with a XhoI restriction site. EcoRI adapters were ligated to the blunt-ended cDNA fragments followed by XhoI digestion. The cDNA fragments were directionally cloned into the EcoRI-XhoI restriction site of the pBluescript vector. The ligated cDNA fragments were transformed into XL10-Gold host cells. This library was constructed by Dr. Lila Vodkin and Dr. Anu Khanna.

text: 2cm long 12 week old

characteristic: lab-host

text: Glycine max

text: EcoRI

text: XhoI

text: young cotyledons of greenhouse grown plants

text: plasmid

text: pBluescript II SK+

characteristic: vector


text: live

text: Generic

characteristic: NCBI submission model

text: Williams

text: This cDNA library was constructed from mRNA isolated from immature cotyledons (100-200mgs) of greenhouse grown plants. The cDNA library was prepared using the Life Technologies pSuperScript cDNA library construction kit. Complementary DNA was synthesized from mRNA using a poly (dT) sequence with a Not I restrictions site. Sal I linkers adapters were ligated to the blunt-ended cDNA fragments followed by NotI digestion. The cDNA fragments were directionally cloned into the NotI-Sal I restriction site of the pSPORT 1 vector. The ligated cDNA fragments were transformed into E. coli ElectroMax DH10B host cells. This library was constructed by Dr. Lila Vodkin and Dr. Anu Khanna.

text: Glycine max

text: EcoRI

text: XhoI

text: immature cotyledons of greenhouse grown plants

text: plasmid

text: pBluescript II SK+


characteristic: External Id

text: live

text: Generic

text: Williams 82

text: This cDNA library was constructed from mRNA isolated from immature flowers of field grown plants. The cDNA library was prepared using the Stratagene pBluescript II XR library construction kit. Complementary DNA was synthesized from mRNA using a primer consisting of a poly (dT) sequence with a XhoI restriction site. EcoRI adapters were ligated to the blunt-ended cDNA fragments followed by XhoI digestion. The cDNA fragments were directionally cloned into the EcoRI-XhoI restriction site of the pBluescript vector. The ligated cDNA fragments were transformed into XL10-Gold host cells. This library was constructed by Dr. Randy Shoemaker and Dr. John Erpelding.

text: Glycine max

text: EcoRI

text: XhoI

text: immature flowers of field grown plants

text: plasmid

text: pBluescript II XR


text: Shanghai Second Medical University, Rui-Jin Hospital

characteristic: INSDC center name

text: live

text: Generic

text: cd34+ stem cell

text: Homo sapiens

text: umbilical cord blood

text: Human mRNA from cd34+ stem cells


text: Rubber Research Institute of Malaysia

characteristic: INSDC first public

text: live

text: Generic

text: Sequence of band retrieved from differential display analysis of Rhizobium sp. NGR234 inoculated and water treated Vigna unguiculata root-hairs

text: Vigna unguiculata

text: root hair

text: Vigna unguiculata root-hair


text: RIKEN

characteristic: INSDC last update

text: live

text: Generic

text: adult

text: Mus musculus

text: male

characteristic: sex

characteristic: strain

text: pancreas

text: Mus musculus pancreas C57BL/6J adult


text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission package

text: Sporozoites were obtained from in vitro sporulated and excysted oocysts of E. tenella grown in chickens. cDNA was synthesized from poly mRNA using an oligo-dT primer containing a XhoI site. Following second strand synthesis, EcoRI adapters were ligated to the cDNA and products were size-selected on Sephacryl S500. cDNAs were digested with EcoRI/XhoI and cloned into lambda Zap II (Stratagene). Clones were converted to phagemids by mass excision using ExAssist helper phage and SOLR cells (Stratagene ). Insert sizes range from 1.2-2.9 kb.

text: Sporozoite

text: SOLR E. coli

text: Eimeria tenella

text: EcoRI

text: XhoI

text: Eimeria S5-2 Sporozoite stage

text: phagemid (Amp. resistant)

text: Bluescript SK-


text: live

text: Generic

text: Merozoites were obtained from ceacal scrapings of chickens infected with E. tenella. The library may contain a small percentage of host or bacterial contaminents. cDNA was synthesized from poly mRNA using an oligo-dT primer containing a XhoI site. Following second strand synthesis, EcoRI adapters were ligated to the cDNA and products were size-selected on Sephacryl S500. cDNAs were digested with EcoRI/XhoI and cloned into lambda Zap II (Stratagene). Clones were converted to phagemids by mass excision using ExAssist helper phage and SOLR cells (Stratagene ). Insert sizes range from 0.7-1.5 kb.

text: Merozoite

text: SOLR E. coli

text: Eimeria tenella

characteristic: organism

text: EcoRI

text: XhoI

text: Eimeria M5-6 Merozoite stage

text: phagemid (Amp. resistant)

text: Bluescript SK-


text: Rubber Research Institute of Malaysia

text: live

text: Generic

text: [Vigna unguiculata (L.) Walp]; cDNA was synthesised from a pooled poly(A)+ RNA prep. from root-hairs collected 24 hr following inoculation with Rhizobium sp. NGR234. XhoI-oligo-dT linker-primer and EcoRI adaptors were used. cDNA was cloned unidirectionally into Uni-ZAP XR (Stratagene, La Jolla, CA), amplified, and excised into pBSK(-) phagemids.

text: Four day old seedling, 24 hours post inoculation with Rhizobium sp. NGR234

text: Vigna unguiculata

text: EcoRI

text: XhoI

text: root-hair

characteristic: tissue-type

text: Vigna unguiculata root-hair Uni-ZAP XR cDNA Library

characteristic: title

text: pBSK(-)


text: University of Chicago Medical Center

text: live

text: Generic

text: epithelium

text: cDNA converted from mRNA of normal colon epithelium cells. These ESTs were identified from a subtracted polydA/dT(-) colon cDNA library. Each sequence is located at the 3'end of the cDNA with a dT16 tag from anchored oligo dT16 RT primer.

characteristic: description

text: Homo sapiens

text: colon

text: polydA/dT(-) colon cDNA library

text: pGEM5zf(+) SphI/NotI


text: live

text: Generic

text: Williams 82

text: This cDNA library was constructed from mRNA isolated from vegatable buds of field grown plants. The cDNA library was prepared using the Stratagene pBluescript II XR library construction kit. Complementary DNA was synthesized from mRNA using a primer consisting of a poly (dT) sequence with a XhoI restriction site. EcoRI adapters were ligated to the blunt-ended cDNA fragments followed by XhoI digestion. The cDNA fragments were directionally cloned into the EcoRI-XhoI restriction site of the pBluescript vector. The ligated cDNA fragments were transformed into XL10-Gold host cells. This library was constructed by Dr. Randy Shoemaker and Dr. John Erpelding.

text: Glycine max

text: EcoRI

text: XhoI

text: vegetable buds of field grown plants

text: plasmid

text: pBluescript II XR


text: RIKEN

text: live

text: Generic

text: adult

characteristic: dev-stage

text: Mus musculus

text: male

text: small intestine

text: Mus musculus small intestine C57BL/6J adult


text: RIKEN

text: live

text: Generic

text: adult

text: Mus musculus

text: liver and lung

text: Mus musculus liver and lung C57BL/6J adult


text: RIKEN

text: live

text: Generic

text: adult

text: Mus musculus

text: male

text: stomach

text: Mus musculus stomach C57BL/6J adult


text: RIKEN

text: live

text: Generic

text: adult

text: Mus musculus

text: male

text: tongue

text: Mus musculus tongue C57BL/6J adult


text: The University of Hong Kong

text: live

text: Generic

text: Mouse oviduct mRNA was isolated at 0, 24, 48, and 72-hrs post h-CG treatment, converted to cDNA and served as tester DNA. Tissues mRNA (liver, kidney, spleen, heart, brain, and lung) from the same mouse were isolated, converted to cDNA and served as driver DNA. A PCR-Selected subtraction kit was used to isolate genes that were differentially expressed in oviduct at different time point. Differentially expressed genes were cloned into pGEM-T easy vector for sequencing.

text: 6-8 weeks

text: Mus musculus

text: female

text: Oviduct

text: Mouse Oviduct cDNA Library


text: National Research Institute of Aquaculture

text: live

text: Generic

text: 30 days after hatching

text: Oryzias latipes

text: female

text: whole body

text: Oryzias latipes whole body 30 days after hatching


text: RIKEN

text: live

text: Generic

text: ES cell

characteristic: cell type

text: Mus musculus

text: Mus musculus C57BL/6J ES cell


text: RIKEN

text: live

text: Generic

text: 13-day embryo

text: Mus musculus

text: mixed

text: liver

text: Mus musculus liver C57BL/6J 13-day embryo


text: RIKEN

text: live

text: Generic

text: 10-day embryo

text: Mus musculus

text: mixed

text: Mus musculus C57BL/6J 10-day embryo


text: The Institute of Physical and Chemical Research (RIKEN)

text: live

text: Generic

text: 11-day embryo

text: Mus musculus

text: mixed

text: Mus musculus C57BL/6J 11-day embryo


text: Clemson University

text: live

text: Generic

characteristic: cultivar

text: supplier: Giovannoni laboratory; cLEC - Cotyledons of seedlings 7-10 days post-germination were excised, cut at both ends and placed on MS medium with no selection. Mixed callus was harvested at 25 and 40 days and included undifferentiated masses. Tomato Callus EST Library

text: 25-40 days old

text: XL1-Blue MRF'

characteristic: lab-host

text: Solanum lycopersicum

text: callus

text: tomato callus, TAMU

text: phagemid

text: pBlueScript SK(-)


text: Clemson University

text: live

text: Generic

characteristic: NCBI submission model

text: cLEE - Tomato Seed EST Library. Directionally cloned cDNAs inserted into pBlueScript SK(-) at 5' end with EcoRI and 3' end with XhoI site.

text: quiescent seed

text: XL1-Blue MRF'

text: Solanum lycopersicum

text: seeds

text: tomato seed, TAMU

text: phagemid

text: pBlueScript SK(-)

characteristic: vector


characteristic: External Id

text: University of California at Davis

characteristic: INSDC center name

text: live

text: Generic

text: Solanum lycopersicum

text: root tip

text: Lycopersicon esculentum root tip


text: Department for Biochemistry

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: spores

characteristic: organ

text: Racocetra castanea

text: asexual

text: Scutellospora castanea Lambda Zap Library


text: RIKEN

text: live

characteristic: INSDC status

text: Generic

text: 10-11 day embryo

text: Mus musculus

text: mixed

characteristic: sex

characteristic: strain

text: Mus musculus C57BL/6J 10-11 day embryo


text: Washington University School of Medicine

text: live

text: Generic

characteristic: NCBI submission package

text: Williams

text: This cDNA library was constructed from mRNA isolated from immature seed coats (200-300 mgs) of greenhouse grown plants. The library was prepared using the Life Technologies pSuperScript cDNA library construction kit. Complementary DNA was synthesized from mRNA using a poly (dT) sequence with a Not I restriction site. Sal I linker adapters were ligated to the blunt-ended cDNA fragments followed by Not I digestion. The cDNA fragments were directionally cloned into the Not I-Sal I restriction site of the pSPORT1 vector. The ligated cDNA fragments were transformed into E.coli ElectroMax DH10B host cells (Gibco BRL). This library was constructed by Dr. Lila Vodkin and Dr. Anu Khanna.

text: DH10B (Gibco BRL)

text: Glycine max

characteristic: organism

text: Not I

text: Sal I

text: immature seed coats of greenhouse grown plants

characteristic: tissue-type

text: plasmid

text: pSPORT1 (Life Technologies)


text: DLO-Centre for Plant Breeding and Reproduction Research (CPRO-DLO)

text: live

text: Generic

text: red fruit

characteristic: dev-stage

text: Fragaria x ananassa

text: Elsanta

text: recaptacle including achenes

text: Strawberry Uni-zapXR cDNA library

characteristic: title


text: Zoology Department, Aberdeen University

text: live

text: Generic

text: Differential display: Biomphalaria glabrata snails exposed to Schistosoma mansoni compared to unexposed snails

characteristic: description

text: adult

text: ovotestis/mantle

text: Biomphalaria glabrata

text: hermaphrodite

text: 1778 Belo Horizonte

text: Biomphalaria glabrata 1778 Belo Horizonte


text: Novartis AG

text: live

text: Generic

text: isolated from mycorrhizal bean roots by mRNA differential display

text: Funneliformis mosseae

text: Glomus mosseae BEG12


text: RIKEN

text: live

text: Generic

text: adult

text: Mus musculus

text: male

text: hippocampus

text: Mus musculus hippocampus C57BL/6J adult


text: Ludwig Institute for Cancer Research

text: live

text: Generic

text: A mini-library was made by cloning products derived from ORESTES PCR (U.S. Letters Patent application No. 196,716 - Ludwig Institute for Cancer Research) profiles into the pUC 18 vector. Reverse transcription of tissue mRNA and cDNA amplification were performed under low stringency conditions.

text: adult

text: colon

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: Ludwig Institute for Cancer Research

text: live

text: Generic

text: adult

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: Ludwig Institute for Cancer Research

text: live

text: Generic

text: adult

text: stomach

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: Beijing Medical University

text: live

text: Generic

text: 22 weeks

text: brain

text: Homo sapiens

text: frontal cortex

text: Human fetal brain Express Library


text: PMBBRC

text: live

text: Generic

text: Brassica rapa subsp. pekinensis

text: Brassica rapa subsp. pekinensis library (Ramesh C)


text: Central South University

text: live

text: Generic

text: Homo sapiens

text: nasopharyngeal carcinoma

text: Homo sapiens nasopharyngeal carcinoma


text: RIKEN

text: live

text: Generic

text: 13-day embryo

text: Mus musculus

text: mixed

text: head

text: Mus musculus head C57BL/6J 13-day embryo


text: RIKEN

text: live

text: Generic

text: 14, 17 day embryo

text: Mus musculus

text: mixed

text: head

text: Mus musculus head C57BL/6J 14, 17 day embryo


text: RIKEN

text: live

text: Generic

text: 12-day embryo

text: Mus musculus

text: mixed

text: head

text: Mus musculus head C57BL/6J 12-day embryo


text: RIKEN

text: live

text: Generic

text: 17-day embryo

text: Mus musculus

text: mixed

text: head

text: Mus musculus head C57BL/6J 17-day embryo


text: RIKEN

text: live

text: Generic

text: adult

text: Mus musculus

text: male

text: brain

text: Mus musculus brain C57BL/6J adult


text: RIKEN

text: live

text: Generic

text: 12-day embryo

text: Mus musculus

text: mixed

text: lower body

text: Mus musculus lower body C57BL/6J 12-day embryo


text: RIKEN

text: live

text: Generic

text: Mus musculus

text: female

text: placenta and extra embryonic tissue

text: Mus musculus placenta and extra embryonic tissue C57BL/6J


text: RIKEN

text: live

text: Generic

text: 14-day embryo

text: Mus musculus

text: mixed

text: liver

text: Mus musculus liver C57BL/6J 14-day embryo


text: RIKEN

text: live

text: Generic

text: adult

text: Mus musculus

text: male

text: testis

text: Mus musculus testis C57BL/6J adult


text: RIKEN

text: live

text: Generic

text: 8-day embryo

text: Mus musculus

text: mixed

text: Mus musculus C57BL/6J 8-day embryo


text: MIPS

text: live

text: Generic

text: adult

characteristic: lab-host

text: Homo sapiens

text: NotI

text: SalI

text: breast cancer

text: 727 (synonym: hmcf1)


text: North Carolina State University

text: live

text: Generic

characteristic: NCBI submission model

text: Differentiating xylem was collected from the main stem of a 35-year old loblolly pine tree harvested during the growing season. RNA isolation and library preparation followed the methods of Allona et al., PNAS 95:9693-8, 1998

text: Pinus taeda

text: EcoRI

text: XhoI

text: differentiating xylem

text: Pine Lambda Zap Xylem library

text: Lambda Zap


text: Bernhard Nocht Institute for Tropical Medicine

text: live

text: Generic

text: Filarial nematode parasite of humans. Two adult female worms of Onchocerca volvulus were isolated from one consenting patient from western Uganda. The patient was treated 28 hours and 7 month before nodulectomy with a single dose of 150 mg/kg ivermectin. Adult female worms were quick frozen. Their mRNA was converted to double-stranded cDNA using reverse transcriptase and oligo(dT) followed by RNase H and DNA pol I. The library has 2.9 x 10E5 independent recombinants and the average insert size is ~800bp. The library was constructed by Peter Fischer with worms provided by Dr. Dietrich W. Buttner. The library is available from Dr.Steven A. Williams (U.S.A.) or Dr. Peter Fischer (Germany), email: genome@smith.edu, Fischpe@aol.com

text: adult

text: XL1-Blue MRF'

text: Onchocerca volvulus

text: Eco RI

text: Xho I

text: female

text: Onchocerca volvulus adult female cDNA following ivermectin (SAW98PF-OvAF)

text: Phage

text: Lambda Uni-ZAP XR

characteristic: vector


characteristic: External Id

text: Dana-Farber Cancer Institute

text: live

text: Generic

text: adult

text: kidney

text: Danio rerio

text: Zebrafish Kidney cDNA random primed, RZPD library no: 576

text: pBK-CMV


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

characteristic: INSDC center name

text: live

text: Generic

text: Double-stranded cDNA was prepared from human fetal brain tissue. 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-GAGAGAGAGAAGAGCTCAAGGATCCTTAATTAAATTAATCCCCCCCCCCCCC-3' and 3' adaptor sequence: 5'-GAGAGAGAGACTCGAGTTTTTTTTTTTTTTTTTT-3'. The library was size-selected for >0.5 kb inserts and has an average insert size estimated at 1.2 kb. This library was constructed using the CAP-trapper method for full-length enrichment and has not undergone amplification. Library was constructed by Dr. Claudio Schneider (LNCIB-Area Science Park, Trieste, Italy).

text: 5 months post-conception

text: brain

text: Homo sapiens

text: SstI

text: XhoI

text: male

text: frontal lobe

text: Schneider fetal brain 00004

text: plasmid (ampicillin resistant)

text: pBluescript SK (Stratagene)


text: Ludwig Institute for Cancer Research

characteristic: INSDC first public

text: live

text: Generic

text: Adult

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: University of Tokyo

characteristic: INSDC last update

text: live

text: Generic

text: obtained by differential display analysis

text: Cucumis sativus

text: Cucumis sativus library (Chono M)


text: Chonbuk National University

text: live

text: Generic

text: Aspergillus niger

text: Aspergillus niger ATCC6275


text: live

text: Generic

text: The NIH_BMAP_MCE library is a non-normalized library constructed from mouse cerebellum. The tag is a string of 5 nucleotides present between the Not I site and the oligo-dT track. The library was constructed as described by Bonaldo, Lennon and Soares, Genome Research 6: 791-806, 1996. Tissue provided by Ms. Annie Novakovich, Zivic-Miller Laboratories.

text: 27-32 days

text: DH10B (Life Technologies)

text: Mus musculus

text: Not I

text: Eco RI

text: Phagemid


text: live

text: Generic

text: The NIH_BMAP_MCE library is a normalized library constructed from mouse cerebellum. The tag is a string of 5 nucleotides present between the Not I site and the oligo-dT track. The library was constructed as described by Bonaldo, Lennon and Soares, Genome Research 6: 791-806, 1996. Tissue provided by Ms. Annie Novakovich, Zivic-Miller Laboratories.

text: 27-32 days

text: DH10B (Life Technologies)

text: Mus musculus

text: Not I

text: Eco RI

text: Phagemid


text: live

text: Generic

text: The NIH_BMAP_MBS library is a non-normalized library constructed from mouse brain stems. The tag is a string of 5 nucleotides present between the Not I site and the oligo-dT track. The library was constructed as described by Bonaldo, Lennon and Soares, Genome Research 6: 791-806, 1996. Tissue provided by Ms. Annie Novakovich, Zivic-Miller Laboratories.

text: 27-32 days

text: DH10B (Life Technologies)

text: Mus musculus

text: Not I

text: Eco RI

text: Phagemid


text: live

text: Generic

text: The NIH_BMAP_MBS_N library is a normalized library constructed from mouse brain stems. The tag is a string of 5 nucleotides present between the Not I site and the oligo-dT track. The library was constructed as described by Bonaldo, Lennon and Soares, Genome Research 6: 791-806, 1996. Tissue provided by Ms. Annie Novakovich, Zivic-Miller Laboratories.

text: 27-32 days

text: DH10B (Life Technologies)

text: Mus musculus

text: Not I

text: Eco RI

text: Phagemid


text: live

text: Generic

text: The NIH_BMAP_MOB library is a non-normalized library constructed from mouse olfactory bulbs. The tag is a string of 5 nucleotides present between the Not I site and the oligo-dT track. The library was constructed as described by Bonaldo, Lennon and Soares, Genome Research 6: 791-806, 1996. Tissue provided by Ms. Annie Novakovich, Zivic-Miller Laboratories.

text: 27-32 days

text: DH10B (Life Technologies)

text: Mus musculus

text: Not I

text: Eco RI

text: Phagemid


text: live

text: Generic

text: The NIH_BMAP_MOB_N library is a normalized library constructed from mouse olfactory bulbs. The tag is a string of 5 nucleotides present between the Not I site and the oligo-dT track. The library was constructed as described by Bonaldo, Lennon and Soares, Genome Research 6: 791-806, 1996. Tissue provided by Ms. Annie Novakovich, Zivic-Miller Laboratories.

text: 27-32 days

text: DH10B (Life Technologies)

text: Mus musculus

text: Not I

text: Eco RI

text: Phagemid


text: live

text: Generic

text: The NIH_BMAP_MHY library is a non-normalized library constructed from mouse hypothalamus. The tag is a string of 5 nucleotides present between the Not I site and the oligo-dT track. The library was constructed as described by Bonaldo, Lennon and Soares, Genome Research 6: 791-806, 1996. Tissue provided by Ms. Annie Novakovich, Zivic-Miller Laboratories.

text: 27-32 days

text: DH10B (Life Technologies)

text: Mus musculus

text: Not I

text: Eco RI

text: Phagemid


text: live

text: Generic

text: The NIH_BMAP_MHY_N library is a normalized library constructed from mouse hypothalamus. The tag is a string of 5 nucleotides present between the Not I site and the oligo-dT track. The library was constructed as described by Bonaldo, Lennon and Soares, Genome Research 6: 791-806, 1996. Tissue provided by Ms. Annie Novakovich, Zivic-Miller Laboratories.

text: 27-32 days

text: DH10B (Life Technologies)

text: Mus musculus

text: Not I

text: Eco RI

text: Phagemid


text: live

text: Generic

text: The NIH_BMAP_MCO library is a non-normalized library constructed from mouse cortex. The tag is a string of 5 nucleotides present between the Not I site and the oligo-dT track. The library was constructed as described by Bonaldo, Lennon and Soares, Genome Research 6: 791-806, 1996. Tissue provided by Ms. Annie Novakovich, Zivic-Miller Laboratories.

text: 27-32 days

text: DH10B (Life Technologies)

text: Mus musculus

text: Not I

text: Eco RI

text: Phagemid


text: live

text: Generic

text: The NIH_BMAP_MCO_N library is a normalized library constructed from mouse cortex. The tag is a string of 5 nucleotides present between the Not I site and the oligo-dT track. The library was constructed as described by Bonaldo, Lennon and Soares, Genome Research 6: 791-806, 1996. Tissue provided by Ms. Annie Novakovich, Zivic-Miller Laboratories.

text: 27-32 days

text: DH10B (Life Technologies)

text: Mus musculus

text: Not I

text: Eco RI

text: Phagemid


text: live

text: Generic

text: The NIH_BMAP_MAM library is a non-normalized library constructed from mouse amygdala. The tag is a string of 5 nucleotides present between the Not I site and the oligo-dT track. The library was constructed as described by Bonaldo, Lennon and Soares, Genome Research 6: 791-806, 1996. Tissue provided by Ms. Annie Novakovich, Zivic-Miller Laboratories.

text: 27-32 days

text: DH10B (Life Technologies)

text: Mus musculus

text: Not I

text: Eco RI

text: Phagemid


text: live

text: Generic

text: The NIH_BMAP_MAM_N library is a normalized library constructed from mouse amygdala. The tag is a string of 5 nucleotides present between the Not I site and the oligo-dT track. The library was constructed as described by Bonaldo, Lennon and Soares, Genome Research 6: 791-806, 1996. Tissue provided by Ms. Annie Novakovich, Zivic-Miller Laboratories.

text: 27-32 days

text: DH10B (Life Technologies)

text: Mus musculus

text: Not I

text: Eco RI

text: Phagemid


text: live

text: Generic

text: The NIH_BMAP_MBG library is a non-normalized library constructed from mouse basal ganglia. The tag is a string of 5 nucleotides present between the Not I site and the oligo-dT track. The library was constructed as described by Bonaldo, Lennon and Soares, Genome Research 6: 791-806, 1996. Tissue provided by Ms. Annie Novakovich, Zivic-Miller Laboratories.

text: 27-32 days

text: DH10B (Life Technologies)

text: Mus musculus

text: Not I

text: Eco RI

text: Phagemid


text: live

text: Generic

text: The NIH_BMAP_MBG_N library is a normalized library constructed from mouse basal ganglia. The tag is a string of 5 nucleotides present between the Not I site and the oligo-dT track. The library was constructed as described by Bonaldo, Lennon and Soares, Genome Research 6: 791-806, 1996. Tissue provided by Ms. Annie Novakovich, Zivic-Miller Laboratories.

text: 27-32 days

text: DH10B (Life Technologies)

text: Mus musculus

text: Not I

text: Eco RI

text: Phagemid


text: live

text: Generic

text: The NIH_BMAP_MPG library is a non-normalized library constructed from mouse pineal gland. The tag is a string of 5 nucleotides present between the Not I site and the oligo-dT track. The library was constructed as described by Bonaldo, Lennon and Soares, Genome Research 6: 791-806, 1996. Tissue provided by Ms. Annie Novakovich, Zivic-Miller Laboratories.

text: 27-32 days

text: DH10B (Life Technologies)

text: Mus musculus

text: Not I

text: Eco RI

text: Phagemid


text: live

text: Generic

text: The NIH_BMAP_MPG_N library is a normalized library constructed from mouse pineal gland. The tag is a string of 5 nucleotides present between the Not I site and the oligo-dT track. The library was constructed as described by Bonaldo, Lennon and Soares, Genome Research 6: 791-806, 1996. Tissue provided by Ms. Annie Novakovich, Zivic-Miller Laboratories.

text: 27-32 days

text: DH10B (Life Technologies)

text: Mus musculus

text: Not I

text: Eco RI

text: Phagemid


text: live

text: Generic

text: The NIH_BMAP_MST library is a non-normalized library constructed from mouse striatum. The tag is a string of 5 nucleotides present between the Not I site and the oligo-dT track. The library was constructed as described by Bonaldo, Lennon and Soares, Genome Research 6: 791-806, 1996. Tissue provided by Ms. Annie Novakovich, Zivic-Miller Laboratories.

text: 27-32 days

text: DH10B (Life Technologies)

text: Mus musculus

text: Not I

text: Eco RI

text: Phagemid


text: live

text: Generic

text: The NIH_BMAP_MST_N library is a normalized library constructed from mouse striatum. The tag is a string of 5 nucleotides present between the Not I site and the oligo-dT track. The library was constructed as described by Bonaldo, Lennon and Soares, Genome Research 6: 791-806, 1996. Tissue provided by Ms. Annie Novakovich, Zivic-Miller Laboratories.

text: 27-32 days

text: DH10B (Life Technologies)

text: Mus musculus

text: Not I

text: Eco RI

text: Phagemid


text: live

text: Generic

text: The NIH_BMAP_MHI library is a non-normalized library constructed from mouse hippocampus. The tag is a string of 5 nucleotides present between the Not I site and the oligo-dT track. The library was constructed as described by Bonaldo, Lennon and Soares, Genome Research 6: 791-806, 1996. Tissue provided by Ms. Annie Novakovich, Zivic-Miller Laboratories.

text: 27-32 days

text: DH10B (Life Technologies)

text: Mus musculus

text: Not I

text: Eco RI

text: Phagemid


text: live

text: Generic

text: The NIH_BMAP_MHI_n library is a normalized library constructed from mouse hippocampus. The tag is a string of 5 nucleotides present between the Not I site and the oligo-dT track. The library was constructed as described by Bonaldo, Lennon and Soares, Genome Research 6: 791-806, 1996. Tissue provided by Ms. Annie Novakovich, Zivic-Miller Laboratories.

text: 27-32 days

text: DH10B (Life Technologies)

text: Mus musculus

text: Not I

text: Eco RI

text: Phagemid


text: live

text: Generic

text: The NIH_BMAP_MSC library is a non-normalized library constructed from mouse spinal cord. The tag is a string of 5 nucleotides present between the Not I site and the oligo-dT track. The library was constructed as described by Bonaldo, Lennon and Soares, Genome Research 6: 791-806, 1996. Tissue provided by Ms. Annie Novakovich, Zivic-Miller Laboratories.

text: 27-32 days

text: DH10B (Life Technologies)

text: Mus musculus

text: Not I

text: Eco RI

text: Phagemid


text: live

text: Generic

text: The NIH_BMAP_MSC_N library is a normalized library constructed from mouse spinal cord. The tag is a string of 5 nucleotides present between the Not I site and the oligo-dT track. The library was constructed as described by Bonaldo, Lennon and Soares, Genome Research 6: 791-806, 1996. Tissue provided by Ms. Annie Novakovich, Zivic-Miller Laboratories.

text: 27-32 days

text: DH10B (Life Technologies)

text: Mus musculus

text: Not I

text: Eco RI

text: Phagemid


text: live

text: Generic

text: The NIH_BMAP_M_S1 library is a subtracted library derived from a mixture of normalized libraries from ten regions of the mouse brain (cerebellum, brain stems, olfactory bulbs, hypothalamus, cortex, amygdala, basal ganglia, pineal gland, striatum, hipoccampus). The driver used for subtraction consisted of a pool of 20,000 cDNA clones obtained from non-normalized and normalized libraries of these ten regions of the mouse brain.

text: 27-32 days

text: DH10B (Life Technologies)

text: Mus musculus

text: Not I

text: Eco RI

text: Phagemid


text: live

text: Generic

text: The NIH_BMAP_M_S2 library is a subtracted library derived from NIH_BMAP_M_S1, which in turn is a subtracted library derived from a mixture of normalized libraries from ten regions of the mouse brain (cerebellum, brain stems, olfactory bulbs, hypothalamus, cortex, amygdala, basal ganglia, pineal gland, striatum, hipoccampus). The driver used for subtraction consisted of a pool of 5,000 clones from the NIH_BMAP_M_S1 library and a pool of 2,000 clones obtained from non-normalized and normalized mouse brain spinal cord libraries.

text: 27-32 days

text: DH10B (Life Technologies)

text: Mus musculus

text: Not I

text: Eco RI

text: Phagemid


text: Istituto di Chimica Biologica 'G. Fornaini'

text: live

text: Generic

text: From differential display

text: Tuber borchii

text: Tuber borchii library (Zeppa S)


text: National Institute of Agricultural Science and Technology

text: live

text: Generic

text: average insert size:1000 bp;initial pfu:1.100 X 10 6

text: XL1-Blue MRF', SOLR

text: Antheraea yamamai

text: EcoRI

text: XhoI

text: Antheraea yamamai posterior silkgland

text: Uni-ZAP XR


text: Centre for Cellular and Molecular Biology

text: live

text: Generic

text: Homo sapiens

text: testis

text: Homo sapiens testis (Singh BN)


text: Georgetown University

text: live

text: Generic

text: macrophage

characteristic: cell type

characteristic: cell-line

text: Mus musculus

text: Mus musculus ANA-1


text: Washington University School of Medicine

text: live

characteristic: INSDC status

text: Generic

text: Williams

characteristic: cultivar

text: This cDNA library was constructed from mRNA isolated from leaves of 2-3 week old greenhouse grown plants. Complementary DNA was synthesized from mRNA using a 3' anchored poly (dT) primer. EcoRI adapters were ligated to the blunt-ended cDNA fragments followed by digestion with EcoRI and HindIII. The cDNA fragments were directionally cloned into the EcoRI-HindIII restriction site of the pT7T3-Pac vector. The ligated cDNA fragments were transformed into DH10B host cells (Gibco BRL). This library was constructed by Dr. Randy Shoemaker and Dr. John Erpelding.

text: Glycine max

text: EcoRI

text: HindIII

text: Leaves, 2-3 week old seedlings, greenhouse grown

text: plasmid

text: pT7T3Pac (pT7T3, Pharmacia)


text: University of Arizona

text: live

text: Generic

characteristic: NCBI submission package

text: epidermal bladder cells

text: Plants stessed 6 weeks in 500mM NaCl

text: 12 weeks old

text: Mesembryanthemum crystallinum

text: EcoRI

text: XhoI

text: MM

text: Plasmid

text: Bluescript SK+


text: University of Edinburgh

text: live

text: Generic

text: Necator americanus is a human hookworm, responsible for debilitating anaemia. The library was constructed from mRNA from N.americanus adults (Nottingham strain) maintained in hamsters.

text: adult

text: Necator americanus

characteristic: organism

text: EcoRI (5'end)

text: XhoI (3'end)

text: mixed

characteristic: sex

text: Necator americanus (parasitic nematode) mixed adult

text: phage

text: Lambda Zap Express


text: live

text: Generic

text: embryo

characteristic: dev-stage

text: Caenorhabditis elegans

text: hermaphrodite

characteristic: strain

text: Strain N2 hermaphrodite embryo


text: Shanghai Institute of Biochemistry, Chinese Academy of Science

text: live

text: Generic

text: Using 24 hour regenerating rat liver tissue after 2/3 partial hepatectomy as the tester and normal rat liver tissue as the driver, suppression subtractive hybridization(SSH) was performed. Then I cloned the cDNA fragments into the pGEM-T vector, and I got a library of about 1000 clones. Part of the clones were identified as expressed differentially between the normal and regenerating liver tissue by differential screening, then they are sequenced.

characteristic: description

text: liver

characteristic: organ

text: Rattus norvegicus

text: male

text: Sprague-Dawley

text: liver

characteristic: tissue-type

text: Rat liver regeneration subtracted cDNA library

characteristic: title

text: plasmid

text: pGEM-T plasmid


characteristic: External Id

text: University of Essen

text: live

text: Generic

characteristic: NCBI submission model

text: At stage 8 ectoderm of Xenopus laevis embryos were cut and disaggregated. The cells were treated with activin at a concentration to induce endodermal tissue. Afterwards the reaggregated cells were cultured until control sibling embryos reaching stage 12/13. An expression library was constructed from such induced endodermal tissue using ZAP Express Kits.

text: Stage 12/13 according to Nieuwkoop and Faber [1967]

text: Xenopus laevis

text: Disaggregated/reaggregated ectoderm treated with activin

text: Xenopus laevis ZAP Express endodermal cDNA library

text: pBK-CMV

characteristic: vector


text: University Essen

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: we cut the ectoderm from embryo of stage 8, disaggregated the tissue, and culture them under the certain concentration of activin, till the sibling develop to stage 13. These tissue were used for cDNA construction.

text: Xenopus laevis

text: EcoRI

text: XhoI

text: activin-induced ectoderm

text: activin-induced ectoderm cDNA library

text: phage

text: pBK-CMV


text: Graduate School of Veterinary Medicine Hokkaido University

text: live

characteristic: INSDC status

text: Generic

text: Mus musculus musculus

text: female

text: CKH

text: Mus musculus musculus CKH E12.5


text: Med. Inst. f. Umwelthygiene

text: live

text: Generic

text: Rattus norvegicus

text: Wistar

text: Rattus norvegicus Wistar (Sandhu HS)


text: University of Essen

text: live

text: Generic

text: Xenopus laevis

text: Xenopus laevis library (Cao Y)


text: IGBMC (Institut de Genetique et de Biologie Moleculaire et Cellulaire)

text: live

text: Generic

characteristic: cell-line

text: Homo sapiens

text: acute promyelocytic leukemia

text: Homo sapiens NB4


text: live

text: Generic

characteristic: NCBI submission package

text: Epithelium

characteristic: cell type

text: Anchor enzyme: NlaIII~ cell culture HMEC-B41 of normal human mammary epithelial cells derived from normal mammoplasty specimen from 51 yo female

text: Breast

text: Homo sapiens

text: Female

text: Normal epithelial cells


text: live

text: Generic

text: Malignant effusion

text: Anchor enzyme: NlaIII~ cell line MDA-MB-453: Human breast carcinoma, malignant effusion. Estrogen receptor negative. From a 48 yo female on radiation and chemotherapy.

text: Breast

text: Homo sapiens

characteristic: organism

text: Female

characteristic: sex

text: Carcinoma


text: live

text: Generic

text: Epithelium

text: Anchor enzyme: NlaIII~ ATCC cell line SK-BR-3. Human breast adenocarcinoma, malignant pleural effusion, estrogen receptor negative. 43 yo female treated with radiation, steroids, cytoxan, 5-fluorouracil.

text: Breast

text: Homo sapiens

text: Female

text: Adenocarcinoma, malignant effusion


text: Institute of Botany

text: live

text: Generic

text: Yanda 1817

characteristic: cultivar

text: Triticum aestivum

text: Triticum aestivum Yanda 1817


text: Institute of Botany

text: live

text: Generic

text: Jingdong No.1

text: Triticum aestivum

text: Triticum aestivum Jingdong No.1


text: Institute of Botany

text: live

text: Generic

text: Daucus carota

text: Daucus carota library (Tan K)


text: Institute of Botany

text: live

text: Generic

text: Arabidopsis thaliana

text: Arabidopsis thaliana library (Tan K)


text: live

text: Generic

text: Anchor enzyme: NlaIII~ This SAGE library is from pooled Ovary cancer cell lines. 'Velculescu, V. E., Zhang, L., Vogelstein, B., and Kinzler, K. W. (1995) Serial Analysis of Gene Expression. Science 270, 484-487'

characteristic: description

text: Homo sapiens

text: Ovary, pooled cancer cell lines


text: live

text: Generic

text: Anchor enzyme: NlaIII~ This SAGE library is from Ovary, normal surface epithelium. 'Velculescu, V. E., Zhang, L., Vogelstein, B., and Kinzler, K. W. (1995) Serial Analysis of Gene Expression. Science 270, 484-487'

text: Homo sapiens

text: Ovary, normal surface epithelium


text: National Institute of Genetics

text: live

text: Generic

text: embryo

characteristic: dev-stage

text: Caenorhabditis elegans

text: hermaphrodite

characteristic: strain

text: Yuji Kohara unpublished cDNA:Strain N2 hermaphrodite embryo


text: National Institutes of Health

text: live

text: Generic

text: adult

text: Mus musculus

text: brain

characteristic: tissue-type

text: Gibco-BRL Cat# 10655-017

characteristic: title


characteristic: External Id

text: Stanford University

text: live

text: Generic

characteristic: NCBI submission model

text: Inbred tassel library from Schmidt lab

text: tassel length from 0.1 to 2.5 cm

text: XLOLR

characteristic: lab-host

text: tassel

characteristic: organ

text: Zea mays

text: tassel

text: 618 - Inbred Tassel cDNA Library

text: phagemid

text: pAD-GAL4-2.1 (Hybrizap)


text: Ludwig Institute for Cancer Research

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: A mini-library was made by cloning products derived from ORESTES PCR (U.S. Letters Patent application No. 196,716 - Ludwig Institute for Cancer Research) profiles into the pUC 18 vector. Reverse transcription of tissue mRNA and cDNA amplification were performed under low stringency conditions.

text: Adult

text: breast

text: Homo sapiens

text: SmaI

text: SmaI

text: female

text: Plasmid

characteristic: vector


text: Tigem (Telethon Institute of Genetics and Medicine)

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission package

text: 19.5 dpc

text: DH10B (ampicillin resistant)

text: Mus musculus

text: total fetus

text: Cot = 5. Library


text: Seoul National University

text: live

text: Generic

text: adult

text: Danio rerio

characteristic: organism

text: brain

text: Danio rerio brain adult


text: Royal Veterinary College

text: live

text: Generic

text: chondrocytes

characteristic: cell type

text: Homo sapiens

text: articular cartilage

text: Homo sapiens articular cartilage


text: Institute of Biomedical Sciences

text: live

text: Generic

text: fetus

text: Homo sapiens

text: lung

text: Homo sapiens lung fetus


text: DIPROVAL Sez.Allevamenti Zootecnici

text: live

text: Generic

text: adult

text: Sus scrofa domesticus

text: skeletal muscle (biceps femoris)


text: Jawaharlal Nehru University

text: live

text: Generic

text: xanthi

characteristic: cultivar

text: Nicotiana tabacum

text: Nicotiana tabacum cv. xanthi differential display


text: Washington University School of Medicine

text: live

text: Generic

text: Williams 82

text: This cDNA library was constructed from mRNA isolated from mature flowers of field grown plants. The cDNA library was prepared using the Stratagene pBluescript II XR cDNA library construction kit. Complementary DNA was synthesized from mRNA using a primer consisting of a poly (dT) sequence with a XhoI restriction site. EcoRI adapters were ligated to the blunt-ended cDNA fragments followed by XhoI digestion. The cDNA fragments were directionally cloned into the EcoRI-XhoI restriction site of the pBluescript vector. The ligated cDNA fragments were transformed into XL10-Gold host cells. This library was constructed by Dr. Randy Shoemaker and Dr. John Erpelding.

characteristic: description

text: Glycine max

text: EcoRI

text: XhoI

text: Mature flowers, field grown plants

text: plasmid

text: pBluescript II XR


text: International Centre for Genetic Engineering and Biotechnology

text: live

text: Generic

text: Pusa bold

text: Brassica juncea

text: Mannitol stress cDNA lib of Brassica juncea cv Pusa bold

text: Lambda ZapII


text: Universidade Federal de Minas Gerais

text: live

text: Generic

text: egg

characteristic: dev-stage

text: Schistosoma mansoni

text: le

characteristic: strain

text: Schistosoma mansoni le egg


text: Universidade Federal de Minas Gerais

text: live

text: Generic

text: adult

text: Schistosoma mansoni

text: le

text: Schistosoma mansoni le adult

characteristic: title


characteristic: External Id

text: Universidade Federal de Minas Gerais

text: live

text: Generic

text: Schistosoma mansoni

text: Schistosoma mansoni library (Rabelo EML)


text: Universidade Federal de Minas Gerais

text: live

text: Generic

characteristic: NCBI submission model

text: miracidia

text: Schistosoma mansoni

text: le

text: Schistosoma mansoni le miracidia


text: Universidade Federal de Minas Gerais

text: live

text: Generic

text: Schistosoma mansoni

text: le

text: lung

characteristic: tissue-type

text: Schistosoma mansoni le lung


text: Medical

text: live

text: Generic

text: Daudi

text: Homo sapiens

text: lymphoma

text: Daudi lymphoma


text: ENSAT-INRA

text: live

text: Generic

text: Evita

text: late immature

text: Solanum lycopersicum

text: green fruit

text: Lycopersicon esculentum green fruit late immature


text: live

text: Generic

text: Williams 82

text: This cDNA library was constructed from mRNA isolated from 2-3 week old greenhouse grown plants.The cDNA library was prepared using the Stratagene pBluescript II XR library construction kit. Complementary DNA was synthesized from mRNA using a primer consisting of a poly (dT) sequence with a XhoI restriction site. EcoRI adapters were ligated to the blunt-ended cDNA fragments followed by XhoI digestion. The cDNA fragments were directionally cloned into the EcoRI-XhoI restriction site of the pBluescript vector. The ligated cDNA fragments were transformed into DH10B host cells (Gibco BRL). This library was constructed by Dr. Randy Shoemaker and Dr. John Erpelding.

text: 2-3 weeks old

text: DH10B (Gibco BRL)

characteristic: lab-host

text: Glycine max

text: EcoRI

text: XhoI

text: leaves of greenhouse grown plants

text: plasmid

text: pBluescript II XR


text: Ludwig Institute for Cancer Research

characteristic: INSDC center name

text: live

text: Generic

text: Adult

text: breast

characteristic: organ

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: Ludwig Institute for Cancer Research

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Adult

text: colon

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: Ludwig Institute for Cancer Research

text: live

characteristic: INSDC status

text: Generic

text: Adult

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid

characteristic: vector


text: Ludwig Institute for Cancer Research

text: live

text: Generic

characteristic: NCBI submission package

text: Adult

text: stomach

text: Homo sapiens

characteristic: organism

text: SmaI

text: SmaI

text: Plasmid


text: University of Helsinki

text: live

text: Generic

text: Galega orientalis

text: Galega orientalis library (Kaijalainen SP)


text: University of Helsinki

text: live

text: Generic

text: Galega orientalis

text: root nodule

text: Galega orientalis root nodule


text: Radiation and Nuclear Safety Authority

text: live

text: Generic

text: epithelial

characteristic: cell type

text: HTori-3, control line

characteristic: cell-line

text: Homo sapiens

text: female

characteristic: sex

text: Thyroid

text: Homo sapiens HTori-3, control line


text: Radiation and Nuclear Safety Authority

text: live

text: Generic

text: epithelial

text: Homo sapiens

text: female

text: Thyroid

text: Homo sapiens HTG9


text: Radiation and Nuclear Safety Authority

text: live

text: Generic

text: epithelial

text: Homo sapiens

text: female

text: Thyroid

text: Homo sapiens HTG8


text: Radiation and Nuclear Safety Authority

text: live

text: Generic

text: epithelial

text: Homo sapiens

text: female

text: Thyroid

text: Homo sapiens HTG13


text: Radiation and Nuclear Safety Authority

text: live

text: Generic

text: epithelial

text: Homo sapiens

text: female

text: Thyroid

text: Homo sapiens HTA4


text: Radiation and Nuclear Safety Authority

text: live

text: Generic

text: epithelial

text: Homo sapiens

text: female

text: Thyroid

text: Homo sapiens HTG2


text: Thomas Jefferson University

text: live

text: Generic

text: Fetal

characteristic: dev-stage

text: Brain

text: Homo sapiens

text: Lambda gt10 human fetal brain

text: Lambda gt10


text: National University of Singapore

text: live

text: Generic

text: Adult

text: Caudal fin

text: Poecilia reticulata

text: Male

text: Red tail variety

characteristic: strain

text: Guppy pGEM-T subtracted library

characteristic: title

text: Plasmid

text: pGEM-T


characteristic: External Id

text: live

text: Generic

characteristic: NCBI submission model

text: Anchor enzyme: NlaIII~ This SAGE library is from primary ovarian tumor, serous adenocarcinoma. 'Velculescu, V. E., Zhang, L., Vogelstein, B., and Kinzler, K. W. (1995) Serial Analysis of Gene Expression. Science 270, 484-487'

characteristic: description

text: Homo sapiens


text: University of Arizona

characteristic: INSDC center name

text: live

text: Generic

text: 5-6 weeks old

text: Mesembryanthemum crystallinum

text: EcorI

text: XhoI

text: Root

characteristic: tissue-type

text: MF

text: Plasmid

text: Bluescript SK+


text: Westfaelische Wilhelms-Univeristaet Muenster

characteristic: INSDC first public

text: live

text: Generic

text: adult

characteristic: lab-host

text: kidney

characteristic: organ

text: Rattus norvegicus

text: Rat Lambda gt11 kidney mesangial cells library

text: lambda gt11


text: West Los Angeles VA Medical Center

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Mus musculus

text: BALB/cByJ

text: liver

text: Mus musculus liver BALB/cByJ


text: Cleveland Clinic

text: live

text: Generic

characteristic: NCBI submission package

text: B-cell leukemia

text: Homo sapiens

characteristic: organism

text: Homo sapiens library (Hawthorn LA)


text: Hainan Medical College

text: live

text: Generic

text: Homo sapiens

text: milk

text: Homo sapiens milk


text: National Cancer Institute

text: live

text: Generic

text: keratinocyte

characteristic: cell type

text: newborn

text: Homo sapiens

text: foreskin epidermal

text: Homo sapiens foreskin epidermal newborn


text: National University of Singapore

text: live

text: Generic

text: Poecilia reticulata

text: Blue tail variety

text: caudal fin

text: Poecilia reticulata Blue tail caudal fin


text: Tong Du Hospital

text: live

text: Generic

text: Homo sapiens

text: osteosarcoma

text: Homo sapiens osteosarcoma differential display


text: NIDDK, National Institutes of Health

text: live

text: Generic

text: Testes dissected from 1-5 day adult y[*] w[67c1]/Y males raised at 25oC. RNA isolated using Trizol (Life Technologies) and a single round of Poly(A)+ selection using Oligotex (Qiagen). cDNA library constructed using Stratagene ZAP-cDNA syntesis kit. Oligo dT-primed, size fractionated ~1-6 kb, and directionally cloned at EcoRI and XhoI in Uni-ZAP XR. Following a single round of amplification pBlueScript SK phagemids were mass excised. A distribution channel for clones is being sought, but not currently available. Requests for clones cannot be honored.

text: 1-5 day adult

text: SOLR (Stratagene)

text: testis

text: Drosophila melanogaster

text: EcoR I

text: Xho I

text: male

characteristic: sex

text: y[*] w[67c1]/Y

text: Drosophila melanogaster adult testis library

text: Plasmid

text: pBlueScript SK (Stratagene)

characteristic: vector


text: live

text: Generic

text: Created by Bento Soares, kindly provided by RZPD, Berlin.

text: adult

characteristic: dev-stage

text: E. coli DH10B

text: kidney

text: Rattus norvegicus

text: EcoRI

text: NotI

text: Zivic-Miller Sprague Dawley

characteristic: strain

text: NbRK Rat Kidney cDNA


characteristic: External Id

text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Library constructed by Life Technologies.

text: breast

text: Homo sapiens

text: SalI

text: NotI

text: four pooled high-grade tumors, including two primary tumors and two metastatic to ovary

characteristic: title

text: phagemid (ampicillin resistant)


text: live

text: Generic

characteristic: NCBI submission model

characteristic: description

text: brain

text: Homo sapiens

text: SalI

text: NotI

text: three pooled meningiomas

text: phagemid (ampicillin resistant)


characteristic: INSDC center name

text: live

text: Generic

text: lung

text: Homo sapiens

text: SalI

text: NotI

text: two pooled squamous cell carcinomas

characteristic: tissue-type

text: phagemid (ampicillin resistant)


characteristic: INSDC first public

text: live

text: Generic

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCGCGACATTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library went through one round of normalization, and was constructed by Bento Soares and M. Fatima Bonaldo.

text: DH10B (phage-resistant)

characteristic: lab-host

text: ovary

text: Homo sapiens

text: Not I

text: Eco RI

text: fibrotheoma

text: phagemid (ampicillin resistant)


characteristic: INSDC last update

text: live

text: Generic

text: ovary

characteristic: organ

text: Homo sapiens

text: SalI

text: NotI

text: normal epithelium

text: phagemid (ampicillin resistant)


text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission package

text: brain

text: Homo sapiens

text: SalI

text: NotI

text: four pooled pituitary adenomas

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: skin

text: Homo sapiens

characteristic: organism

text: SalI

text: NotI

text: malignant melanoma, metastatic to lymph node

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: lung

text: Homo sapiens

text: SalI

text: NotI

text: four pooled poorly-differentiated adenocarcinomas

text: phagemid (ampicillin resistant)


text: The Institute for Genomic Research

text: live

text: Generic

text: This library was constructed by directed cloning into the EcoRI-XhoI sites of pAD-GAL4.Estimated Average insert size approximately 1.2kb based on 15 clones, estimated titer of excised phagemid library = 3.78 x 106 cfu/ml

text: maternal

text: XL1-Blue MRF'

text: Danio rerio

text: whole body

text: zebrafish, Steve Ekker

text: phagemid


text: Dana-Farber Cancer Institute

text: live

text: Generic

text: adult

text: kidney

text: Danio rerio

text: RZPD library 575


text: National University of Singapore

text: live

text: Generic

text: adult

text: Poecilia reticulata

text: Red tail variety

text: caudal fin

text: Poecilia reticulata Red tail caudal fin


text: Cancer Research Institution of HuNan Medical University

text: live

text: Generic

text: adult

text: Homo sapiens

text: larynx

text: Homo sapiens larynx adult


text: live

text: Generic

text: Columbia

characteristic: cultivar

text: Arabidopsis thaliana

text: Arabidopsis thaliana library (Nambara E)


text: Universita' di Pavia

text: live

text: Generic

text: astrocytoma

characteristic: cell type

text: Homo sapiens

text: Homo sapiens astrocytoma library


text: Universita' di Pavia

text: live

text: Generic

text: non-tumorogenic

text: Homo sapiens

text: Homo sapiens non-tumorogenic library


text: Universita' di Pavia

text: live

text: Generic

text: glioblastoma

text: Homo sapiens

text: Homo sapiens glioblastoma library


text: Department of Genetics, The Hospital for Sick Children

text: live

text: Generic

text: Homo sapiens

text: Homo sapiens library (Scherer SW)


text: Oklahoma State University

text: live

text: Generic

text: PI 437654

text: Glycine max

text: Glycine max PI 437654 library


text: University of Aarhus

text: live

text: Generic

text: variety Gifu B-129

text: Two times polyA+ purified mRNA, oligodT primed, 5' adapter tag GAATTCGGCACGAG

text: young plants

characteristic: dev-stage

text: Escherichia coli XL-1Blue MRF'

text: whole roots and root nodule primordia

text: Lotus japonicus

text: EcoRI

text: XhoI

text: Ljirnp Lambda HybriZap two-hybrid library

text: plasmid

characteristic: vector


characteristic: External Id

text: Guys Hospital

text: live

text: Generic

text: fetus

text: Homo sapiens

text: liver

text: Homo sapiens liver fetus


text: Guys Hospital

text: live

text: Generic

text: Homo sapiens

characteristic: title


text: Guys Hospital

text: live

text: Generic

characteristic: NCBI submission model

text: adult

text: Homo sapiens

text: muscle

text: Homo sapiens muscle adult


text: University of Houston

text: live

text: Generic

text: adult

text: Drosophila melanogaster

text: Canton S

characteristic: strain

text: brain

text: Drosophila melanogaster brain Canton S adult


text: Fourth Military Medical University

characteristic: INSDC center name

text: live

text: Generic

text: Homo sapiens

text: Homo sapiens differential display PCR (Xian B)


text: Pennsylvania State University College of Medicine

text: live

text: Generic

text: ATCC SW1088

characteristic: cell-line

text: brain

text: Homo sapiens

text: astrocytoma

characteristic: tissue-type

text: Homo sapiens ATCC SW1088


text: Washington University School of Medicine

characteristic: INSDC first public

text: live

text: Generic

text: Williams

text: This cDNA library was constructed from mRNA isolated from nodules on the roots of 2.5 month-old Glycine max 'Williams' plants that were greenhouse grown. The The cDNA library was prepared using the Stratagene pBluescript II SK(+) library construction kit. First strand synthesis was performed with 5-methyl dCTP, hence the ligated cDNA was hemimethylated. A modification of Stratagene's first-strand synthesis primer was used. An 'anchor' nucleotide (v=A, C, or G) was added to the 3' end of the primer [GAGAGAGAGAGAGAGAGAGAACTAGTCTCGAG(T)18V] to anchor the primer at the 5' end of the poly(A) tract. After second-strand synthesis, the cDNA ends were filled in with cloned Pfu DNA polymerase and size- fractionated with a 400 bp cutoff, using a SizeSep 400 Spun column from Pharmacia. The column eluent was ligated to EcoRI adaptors and phosphporylated. The XhoI sites in the cDNA would be protected by their hemimethylated status. The cDNA constructs were size- fractionated with a 500 bp cutoff, using GibcoBRL Life Technologies' cDNA Size Predigested vector (pBluescript II SK+) that has been digested with EcoRI and XhoI, and phosphorylated by Stratagene. Both the white and blue colonies appear to contain recombinant plasmids with cDNA inserts, based on size (n=56) and sequence (n=16). This library was constructed by Dr. Paul Keim and Dr. Virginia Coryell.

characteristic: description

characteristic: lab-host

text: Glycine max

text: EcoRI

text: XhoI

text: root nodules of greenhouse grown plants

text: plasmid

text: pBluescript II SK+


text: Institute of Basic Medical Sciences, Beijing

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: endothelia

text: with treatment by endostatin, the differential expressed cDNAs were cloned into the pGEM-T vector. The 3'anchor primer is AAGCTTTTTTTTTTT, and the 5'arbitrary primer is 10-mer oligo. Individual clone was sequenced with T7 promoter primer.

text: treated by endostatin for 12 hours

text: brain

characteristic: organ

text: Rattus norvegicus

text: micro-vascular

text: Differential expressed library with endostatin

text: plasmid

text: pGEM-T


text: live

text: Generic

characteristic: NCBI submission package

text: Anchor enzyme: NlaIII~ This SAGE library is well-differentiated fibrillary astrocytoma derived from 50-yr old female. 'Velculescu, V. E., Zhang, L., Vogelstein, B., and Kinzler, K. W. (1995) Serial Analysis of Gene Expression. Science 270, 484-487'

text: 50-yr old

text: Homo sapiens

characteristic: organism

text: female

characteristic: sex


text: The Institute for Genomic Research

text: live

text: Generic

text: directionally cloned cDNAs

text: Schistosoma mansoni

text: EcoRI

text: XhoI

text: Normalized Schistosoma mansoni, Phil LoVerde/Joe Merrick

text: pBluescript SK

characteristic: vector


characteristic: External Id

text: The Institute for Genomic Research

text: live

text: Generic

text: directionally cloned cDNAs

text: Schistosoma mansoni

text: EcoRI

text: XhoI

text: female

text: Schistosoma mansoni female, Phil LoVerde/Joe Merrick

text: pBluescript SK


text: The Institute for Genomic Research

text: live

text: Generic

text: directionally cloned cDNAs

text: Schistosoma mansoni

text: EcoRI

text: XhoI

text: male

text: Schistosoma mansoni male, Phil LoVerde/Joe Merrick

characteristic: title

text: pBluescript SK


text: Northeast Agricultural University

text: live

text: Generic

characteristic: NCBI submission model

text: Shenxianggeng No.4 sub-species Japonica

characteristic: cultivar

text: Prepared from leaf mRNA of rice seedlings infected with blast fungus by using the 3' one base-anchored poly-dT primers provided in differential display kit. The cDNAs of interest were identified and cloned into pGEM-T vector before they were sequenced.

text: 3 week old seedling

characteristic: dev-stage

text: Oryza sativa

text: Leaf

text: Rice leaf express library

text: plasmid

text: pGEM-T


text: Stanford University

characteristic: INSDC center name

text: live

text: Generic

text: Anther and pollen cDNA library. Directionally sequenced with 5' end at the EcoRI site. Created by Amie Franklin.

text: premieotic anthers to pollen shed

text: XLOLR

text: anthers

text: Zea mays

text: EcoRI

text: XhoI

text: whole premieotic anthers to pollen shed

text: 660 - Mixed stages of anther and pollen

text: plasmid

text: Lambda Zap


text: Universita di Milano

text: live

text: Generic

text: IL-2 activated lymphocytes

characteristic: cell type

text: Ovis aries

text: blood

characteristic: tissue-type

text: Ovis aries blood


text: Department of Rheumatology, University Hospital

characteristic: INSDC first public

text: live

text: Generic

text: Synovial fibroblasts

text: Isolated from ultracentrifuged synovial fluid pellet obtained from a patient with rheumatoid arthritis

characteristic: description

text: Knee

text: Homo sapiens

text: female

text: Synovial tissue

text: Synovial fibroblasts, rheumatoid arthritis


text: Mayo Clinic Cancer Center

characteristic: INSDC last update

text: live

text: Generic

text: LNCaP

characteristic: cell-line

text: prostate cancer metastasized to lymph node; subtracted cDNA libraries from prostate cancer cell line LNCaP treated with androgen.

text: Homo sapiens

text: prostate cancer cell line LNCaP


text: Genome Systems, Inc., a wholly owned subsidiary of Incyte Pharmaceuticals, Inc.

text: live

characteristic: INSDC status

text: Generic

text: Columbia Col-0

text: cDNA library was derived from untreated inflorescence tissue from Arabidopsis thaliana, Columbia Col-0, at 4 - 7 weeks. Plants were grown in 1:1:1 peat moss/vermiculite/perlite soil at 22 deg. C +/- 3 deg. C under constant light, and watered with fertilizer. cDNA synthesis was initiated using a NotI-oligo(dT) primer. Double-stranded cDNA was blunted, ligated to SalI adaptors, digested with NotI, size-selected, and cloned into the NotI and SalI sites of the pSPORT vector.

text: 4 - 7 weeks

text: Arabidopsis thaliana

text: NotI

text: SalI

text: inflorescence

text: A. thaliana, Columbia Col-0, inflorescence-1

text: pSPORT


text: live

text: Generic

characteristic: NCBI submission package

text: Columbia Col-0

text: 4 - 7 weeks

text: Arabidopsis thaliana

text: NotI

text: SalI

text: inflorescence

text: A. thaliana, Columbia Col-0, inflorescence-2

text: pSPORT


text: live

text: Generic

text: Columbia Col-0

text: cDNA library was derived from untreated root tissue from Arabidopsis thaliana, Columbia Col-0, at 4 - 7 weeks. Plants were grown in 1:1:1 peat moss/vermiculite/perlite soil at 22 deg. C +/- 3 deg. C under constant light, and watered with fertilizer. cDNA synthesis was initiated using a NotI-oligo(dT) primer. Double-stranded cDNA was blunted, ligated to SalI adaptors, digested with NotI, size-selected, and cloned into the NotI and SalI sites of the pSPORT vector.

text: 4 - 7 weeks

text: Arabidopsis thaliana

characteristic: organism

text: NotI

text: SalI

text: root

text: A. thaliana, Columbia Col-0, root-1

text: pSPORT


text: live

text: Generic

text: Columbia Col-0

text: 4 - 7 weeks

text: Arabidopsis thaliana

text: NotI

text: SalI

text: root

text: A. thaliana, Columbia Col-0, root-2

text: pSPORT


text: live

text: Generic

text: Columbia Col-0

text: cDNA library was derived from untreated rosette tissue from Arabidopsis thaliana, Columbia Col-0, at 4 - 7 weeks. Plants were grown in 1:1:1 peat moss/vermiculite/perlite soil at 22 deg. C +/- 3 deg. C under constant light, and watered with fertilizer. cDNA synthesis was initiated using a NotI-oligo(dT) primer. Double-stranded cDNA was blunted, ligated to SalI adaptors, digested with NotI, size-selected, and cloned into the NotI and SalI sites of the pSPORT vector.

text: 4 - 7 weeks

text: Arabidopsis thaliana

text: NotI

text: SalI

text: rosette

text: A. thaliana, Columbia Col-0, rosette-1

text: pSPORT


text: live

text: Generic

text: cDNA library was made from selected clones from the Arabidopsis thaliana Ohio State clone set.

text: Arabidopsis thaliana

text: A. thaliana, Ohio State clone set


text: live

text: Generic

text: Columbia Col-0

text: 4 - 7 weeks

text: Arabidopsis thaliana

text: NotI

text: SalI

text: rosette

text: A. thaliana, Columbia Col-0, rosette-2

text: pSPORT


text: live

text: Generic

text: Columbia Col-0

text: 4 - 7 weeks

text: Arabidopsis thaliana

text: NotI

text: SalI

text: rosette

text: A. thaliana, Columbia Col-0, rosette-3

text: pSPORT


text: live

text: Generic

text: This sequence was obtained from a clone generated with a PCR product of the target gene.

text: Arabidopsis thaliana

text: A. thaliana, mixed source


text: Fourth Military Medical University

text: live

text: Generic

characteristic: lab-host

text: Homo sapiens

text: Homo sapiens cell line K562 cDNA Library

text: Plasmid


text: Institute of Basic Medical Sciences

text: live

text: Generic

text: Oryctolagus cuniculus

text: dorsal root ganglion

text: Oryctolagus cuniculus dorsal root ganglion


text: All Children's Hospital

text: live

text: Generic

text: adult

text: Danio rerio

text: kidney

text: Danio rerio kidney adult


text: Cancer Research Institute

text: live

text: Generic

text: Homo sapiens

text: Homo sapiens library (Jangbo Y)


text: live

text: Generic

text: Anchor enzyme: NlaIII~ This SAGE library is a prostate cancer cell line, androgren-dependent, with inactivating mutation in PTEN gene. 'Velculescu, V. E., Zhang, L., Vogelstein, B., and Kinzler, K. W. (1995) Serial Analysis of Gene Expression. Science 270, 484-487'

text: Homo sapiens

text: prostate cancer cell line


text: Thomas Jefferson University

text: live

text: Generic

text: Adult

text: Liver

characteristic: organ

text: Homo sapiens

text: Lambda gt10 human adult liver


characteristic: External Id

text: North Carolina State University

text: live

text: Generic

text: Immature pollen cones were collected in the early spring, frozen and used for mRNA isolation. The SMART-PCR method (Clontech) was used to prepare a library from 1 ug total RNA, using the Lambda TriplEx vector. Plasmid subclones in pTriplEx were recovered by cre-lox excision in E. coli strain BM25.8 and sequenced from the 5' end.

text: immature

text: E. coli BM25.8

text: pollen cone

text: Pinus taeda

text: SfiI (A)

text: SfiI (B)

text: Pine TriplEx pollen cone library

text: Phage

text: Lambda TriplEx

characteristic: vector


text: North Carolina State University

text: live

text: Generic

characteristic: NCBI submission model

text: Shoot tips (approx. 2 cm from apex) were collected during the spring, frozen and used for mRNA isolation. The SMART-PCR method (Clontech) was used to prepare a library from 1 ug total RNA, using the Lambda TriplEx vector. Plasmid subclones in pTriplEx were recovered by cre-lox excision in E. coli strain BM25.8 and sequenced from the 5' end.

text: E. coli BM25.8

text: shoot tips

text: Pinus taeda

text: SfiI (A)

text: SfiI (B)

text: Pine TriplEx shoot tip library

characteristic: title

text: Phage

text: Lambda TriplEx


text: NRC Institute for Marine Biosciences

text: live

text: Generic

text: adult

characteristic: dev-stage

text: liver

text: Pseudopleuronectes americanus

text: female

characteristic: sex

text: Winter flounder liver


text: NRC Institute for Marine Biosciences

characteristic: INSDC center name

text: live

text: Generic

text: adult

text: ovary

text: Pseudopleuronectes americanus

text: female

text: Winter flounder ovary


text: NRC Institute for Marine Biosciences

text: live

text: Generic

text: adult

text: stomach

text: Pseudopleuronectes americanus

text: female

text: Winter flounder stomach


text: NRC Institute for Marine Biosciences

text: live

text: Generic

text: adult

text: spleen

text: Pseudopleuronectes americanus

text: female

text: Winter flounder spleen


text: NRC Institute for Marine Biosciences

text: live

text: Generic

text: adult

text: pyloric caeca

text: Pseudopleuronectes americanus

text: female

text: Winter flounder pyloric caeca


text: NRC Institute for Marine Biosciences

text: live

text: Generic

text: adult

text: intestine

text: Pseudopleuronectes americanus

text: female

text: Winter flounder intestine


text: Cornell University

text: live

text: Generic

text: Brooks

characteristic: cultivar

text: A Uni-ZAP XR cDNA library was constructed from etiolated leaf mRNA from the oat cultivar 'Brooks' and converted to pBluescript (amp resistant) as described in Heun et al. (1991) Genome 34:437-447. For insert amplification, use M13 forward and reverse primers. Clones from this library are designated with the prefix 'CDO'

text: Avena sativa

text: EcoRI

text: XhoI

text: Oat Etiolated Leaf cDNA Library

text: Uni-ZAP XR/pBluescript


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

characteristic: INSDC first public

text: live

text: Generic

text: The NCI_CGAP_Sub1 library is a subtracted library derived from BI. BI constitutes a mixture of 21 normalized or subtracted NCI_CGAP libraries: NCI_CGAP_Co4, NCI_CGAP_Pr22, NCI_CGAP_Pr28, NCI_CGAP_Co10, NCI_CGAP_Co16, NCI_CGAP_Kid5, NCI_CGAP_Kid12, NCI_CGAP_Kid3, NCI_CGAP_Kid11, NCI_CGAP_Lym2, NCI_CGAP_Br2, NCI_CGAP_Co8, NCI_CGAP_CLL1, NCI_CGAP_Lei2, NCI_CGAP_Brn23, NCI_CGAP_Lu5, NCI_CGAP_Lu24, NCI_CGAP_Lu19, NCI_CGAP_GC4, NCI_CGAP_GC6, NCI_CGAP_Brn25. These 21 libraries were pooled and a single-stranded DNA preparation of the resulting mixture was used as a tracer in a subtractive hybridization with a driver whose composition is detailed below: NCI_CGAP_Kid3 pool 1 LLAM 3334-3337, 3682-3683, 3798-3803 (IMAGE CloneIDs 1322376-1323911, 1456008-1456775, 1500552-1502855) NCI_CGAP_Kid5 pool 1 LLAM 3338-3342, 3722-3725, 3776-3778 (IMAGE CloneIDs 1323912-1325831, 1471368-1472903, 1492104-1493255) NCI_CGAP_Lu5 pool 1 LLAM 3575-3582, 3851-3854 (IMAGE CloneIDs 1414920-1417991, 1520904-1522439) NCI_CGAP_GC4 pool 1 LLAM 3164-3167, 3716-3720, 3733-3735 (IMAGE CloneIDs 1257096-1258631, 1469064-1470983, 1475592-1476743) NCI_CGAP_Pr22 pool 1 LLAM 2457-2459, 2758-2759, 3062-3068 (IMAGE CloneIDs 985608-986759, 1101192-1101959, 1217928-1220615) NCI_CGAP_Co10 pool 1 LLAM 2644-2653, 2871-2872 (IMAGE CloneIDs 1057416-1061255, 1144584-1145351) The resulting subtracted library contained 530,000 recombinants. Subtraction was performed as previously described [Bonaldo, Lennon & Soares (1996): Normalization and Subtraction: Two Approaches To Facilitate Gene Discovery. Genome Research 6, 791-806.

text: DH10B (Life Technologies)

text: Homo sapiens

text: Not I

text: Eco RI

text: Phagemid


text: live

text: Generic

text: The NCI_CGAP_Sub2 library is a subtracted library derived from BI. BI constitutes a mixture of 21 normalized or subtracted NCI_CGAP libraries: NCI_CGAP_Co4, NCI_CGAP_Pr22, NCI_CGAP_Pr28, NCI_CGAP_Co10, NCI_CGAP_Co16, NCI_CGAP_Kid5, NCI_CGAP_Kid12, NCI_CGAP_Kid3, NCI_CGAP_Kid11, NCI_CGAP_Lym2, NCI_CGAP_Br2, NCI_CGAP_Co8, NCI_CGAP_CLL1, NCI_CGAP_Lei2, NCI_CGAP_Brn23, NCI_CGAP_Lu5, NCI_CGAP_Lu24, NCI_CGAP_Lu19, NCI_CGAP_GC4, NCI_CGAP_GC6, NCI_CGAP_Brn25. These 21 libraries were pooled and a single-stranded DNA preparation of the resulting mixture was used as a tracer in a subtractive hybridization with a driver whose composition is detailed below: NCI_CGAP_Kid3 pool 1 LLAM 3334-3337, 3682-3683, 3798-3803 (IMAGE CloneIDs 1322376-1323911, 1456008-1456775, 1500552-1502855) NCI_CGAP_Kid5 pool 1 LLAM 3338-3342, 3722-3725, 3776-3778 (IMAGE CloneIDs 1323912-1325831, 1471368-1472903, 1492104-1493255) NCI_CGAP_Lu5 pool 1 LLAM 3575-3582, 3851-3854 (IMAGE CloneIDs 1414920-1417991, 1520904-1522439) NCI_CGAP_GC4 pool 1 LLAM 3164-3167, 3716-3720, 3733-3735 (IMAGE CloneIDs 1257096-1258631, 1469064-1470983, 1475592-1476743) NCI_CGAP_Pr22 pool 1 LLAM 2457-2459, 2758-2759, 3062-3068 (IMAGE CloneIDs 985608-986759, 1101192-1101959, 1217928-1220615) NCI_CGAP_Co10 pool 1 LLAM 2644-2653, 2871-2872 (IMAGE CloneIDs 1057416-1061255, 1144584-1145351) The resulting subtracted library contained 4 million recombinants. Subtraction was performed as previously described [Bonaldo, Lennon & Soares (1996): Normalization and Subtraction: Two Approaches To Facilitate Gene Discovery. Genome Research 6, 791-806.

characteristic: description

text: DH10B (Life Technologies)

text: Homo sapiens

text: Not I

text: Eco RI

text: Phagemid


text: Thomas Jefferson University

characteristic: INSDC last update

text: live

text: Generic

text: Fetal

text: Brain

text: Homo sapiens

text: 18p11 cDNA selection library


text: Kyungpook National University School of Medicine

text: live

text: Generic

text: trophozoite

text: XL1 blue

text: Entamoeba histolytica

text: EcoRI

text: XhoI

characteristic: strain

text: Entamoeba histolytica trophozoite

text: Phage

text: Lambda ZAP Express


text: live

characteristic: INSDC status

text: Generic

text: 30-45 DAF

text: Brassica juncea

text: variety Pusa Jaikishan

text: developing seed

characteristic: tissue-type

text: Brassica juncea developing seed 30-45 DAF


text: live

text: Generic

characteristic: NCBI submission package

text: 25-45 DAF

text: Brassica juncea

characteristic: organism

text: variety Pusa Jaikishan

text: developing seed

text: Brassica juncea developing seed 25-45 DAF


text: IBVM, INRA-CR Bordeaux

text: live

text: Generic

text: WVA 106

text: adult

text: Escherichia coli JM109

characteristic: lab-host

text: flower and fruit

characteristic: organ

text: Solanum lycopersicum

text: hermaphrodite

text: Sweet Cherry

text: pBSLe

text: Plasmid

text: pBlueScript


characteristic: External Id

text: live

text: Generic

text: Anchor enzyme: NlaIII~ This SAGE library is from Brain, c-myc positive medulloblastoma cell line H341. 'Velculescu, V. E., Zhang, L., Vogelstein, B., and Kinzler, K. W. (1995) Serial Analysis of Gene Expression. Science 270, 484-487'

text: Homo sapiens


text: Clemson University

text: live

text: Generic

characteristic: NCBI submission model

text: cLEF - Fruit were tagged at the 1cm stage and harvested 3-5 days prior to ripening. Fruit were cut in half to verify the seeds were indeed 'immature' and the seeds and locules were discarded prior to freezing the pericarp

text: mature green (3-5 days pre-ripening)

text: SOLR

text: Solanum lycopersicum

text: fruit pericarp

text: tomato fruit mature green, TAMU

text: phagemid

text: pBlueScript SK(-)

characteristic: vector


text: live

text: Generic

text: Rio Grande PtoR

text: cLET - Inoculated with a variety of disease response elicitors. Plants exposed to 2,6 dichloroisonicotinic acid, BTH, jasmonic acid, ethylene, fenthion, EIX, okadaic acid, or systemin prior to tissue harvest. EcoRI site was destroyed during cloning.

text: 4-6 week old plants

characteristic: dev-stage

text: XL1-Blue MRF'

text: Solanum lycopersicum

text: leaf

text: tomato mixed elicitor, cmr

characteristic: title

text: phagemid

text: pBlueScript SK(-)


text: Hokkaido University

characteristic: INSDC center name

text: live

text: Generic

text: Columbia

text: This sequence is obtained from a PCR product generated by differential display method. cDNA prepared from immature siliques (10 days after flowering) was used as the template

text: 10 days after flowering

text: Arabidopsis thaliana

text: immature siliques

text: Arabidopsis thaliana immature siliques


text: Hokkaido University

text: live

text: Generic

text: Columbia

characteristic: cultivar

text: Arabidopsis thaliana

text: germinating seeds

text: Arabidopsis thaliana germinating seeds

text: lambda Uni-ZAPII


text: Smith College Department of Biological Sciences

characteristic: INSDC first public

text: live

text: Generic

text: Lymphatic filarial nematode parasite of humans. mRNA was prepared from L4s isolated from the peritoneal cavity of jirds and converted to double-stranded cDNA using reverse transcriptase and oligo(dT) followed by RNase H and DNA pol I. The library has 2.7 x 105 independent recombinants and the average insert size is approx. 1050bp. The library was constructed by Michelle Lizotte-Waniewski. The library is available from Dr. S.A. Williams, email:genome@neal.smith.edu.

text: larval stage four

text: XL1-Blue MRF'

text: Brugia malayi

text: Eco RI

text: Xho I

text: Brugia malayi L4 cDNA (SAW99MLW-BmL4)

text: Phage

text: Lambda Uni-ZAP XR


text: live

text: Generic

text: Lymphatic filarial nematode parasite of humans. mRNA was prepared from young adult worms isolated from the peritoneal cavity of jirds and converted to double-stranded cDNA using reverse transcriptase and oligo(dT) followed by RNase H and DNA pol I. The library has 6.5 x 104 independent recombinants and the average insert size is approx. 800bp. The library was constructed by Michelle Lizotte-Waniewski. The library is available from Dr. S.A. Williams, email: genome@neal.smith.edu.

characteristic: description

text: young adult

text: XL1-Blue MRF'

text: Brugia malayi

text: Eco RI

text: Xho I

text: Brugia malayi young adult cDNA (SAW99MLW-BmYA)

text: Phage

text: Lambda Uni-ZAP XR


text: The Institute of Physical and Chemical Research

characteristic: INSDC last update

text: live

text: Generic

text: Arabidopsis thaliana

text: Columbia

text: Lambda ZipLox designated PRL2


text: University of Massachusetts

text: live

text: Generic

text: putative thyroid hormone-responsive mRNA isolated by differential display RT-PCR

text: Rattus norvegicus

text: Sprague-Dawley

characteristic: strain

text: cerebral cortex

text: Rattus norvegicus cerebral cortex Sprague-Dawley GD16


text: University of Southern Mississippi, Center for Molecular & Cellular Biosciences

text: live

characteristic: INSDC status

text: Generic

text: Histoplasma capsulatum

text: Histoplasma capsulatum G186AS


text: University of Nijmegen

text: live

text: Generic

text: Homo sapiens

text: 530 melanoma

characteristic: tissue-type

text: Homo sapiens 530 melanoma


text: University of Nijmegen

text: live

text: Generic

characteristic: NCBI submission package

text: Homo sapiens

text: MV3 melanoma

text: Homo sapiens MV3 melanoma


text: Ludwig Institute for Cancer Research

text: live

text: Generic

text: A mini-library was made by cloning products derived from ORESTES PCR (U.S. Letters Patent application No. 196,716 - Ludwig Institute for Cancer Research) profiles into the pUC 18 vector. Reverse transcription of tissue mRNA and cDNA amplification were performed under low stringency conditions.

text: Adult

text: breast

text: Homo sapiens

characteristic: organism

text: SmaI

text: SmaI

text: Plasmid


text: Oklahoma State University

text: live

text: Generic

text: From Jacqueline Fletcher's laboratory

text: adult

text: Neoaliturus tenellus

text: whole body


text: Karolinska Institute

text: live

text: Generic

text: Hepatic cDNA obtained from vehicle or GH-treated old male rats were used as driver (vehicle) and tester (GH), or vice versa, to generate gene products that were induced or repressed by GH. After two rounds of subtraction and amplification, using hybridization tester-driver ratios of 1:100 and 1:800, difference products were cloned in the BamH1 site of the pBluescript II SK+ vector.

text: 2 year old

text: Rattus norvegicus

text: BamHI

text: BamHI

text: male

characteristic: sex

text: Sprague-Dawley

text: liver

text: Representational Difference Analysis Products

text: pBluescript II SK+


text: Genoscope - CNS

text: live

text: Generic

text: Cloned unidirectionally.~ High quality sequence only submitted.~ Average insert size: 1.2 kb (range: 0.2 - 2.kb)

text: 7.5 dpc

text: Mus musculus

text: NotI

text: SalI

text: C57BL/6 x DBA

text: dissected endoderm

text: Beddington mouse dissected endoderm


text: Biology, University of New Mexico

text: live

text: Generic

text: cDNA library representing mRNA from M-line Biomphalaria glabrata harboring 4-day old Echinostoma paraensei infection

text: Biomphalaria glabrata

text: M-line

text: Biomphalaria glabrata M-line


text: Princeton University

text: live

text: Generic

text: colon cancer

characteristic: cell type

characteristic: cell-line

text: Homo sapiens

text: Homo sapiens EB1


characteristic: External Id

text: University of Massachusetts Medical School

text: live

text: Generic

characteristic: NCBI submission model

text: Homo sapiens

text: normal skeletal muscle

text: normal muscle cDNA subtractive library


text: Chinese Academy of Science

text: live

text: Generic

text: cloned from rat brains of different developmental stages

text: Rattus norvegicus

text: Sprague-Dawley

text: brain

text: Rat brain ESTs


text: live

text: Generic

text: Homo sapiens

text: facioscapulohumeral dystrophic muscle

text: FSHD affected muscle cDNA subtractive library


text: Lawrence Livermore National Laboratory

text: live

text: Generic

text: This is a directionally cloned cDNA library from adult Zebrafish olfactory epithelium.

text: adult

characteristic: dev-stage

text: D10Hb (Gibco BRL)

characteristic: lab-host

text: Danio rerio

text: NotI

text: SalI

text: mixed

text: Olfactory rosettes

text: Zebrafish adult olfactory

text: phagemid

characteristic: vector


text: University of Agricultural Sciences

characteristic: INSDC center name

text: live

text: Generic

text: ovary

text: CHO 2F5

text: Cricetulus griseus

text: Cricetulus griseus CHO 2F5

characteristic: title


text: MIPS

characteristic: INSDC first public

text: live

text: Generic

text: adult

text: Homo sapiens

text: NotI

text: SalI

text: amygdala

text: 761 (synonym: hamy2)


text: MIPS

characteristic: INSDC last update

text: live

text: Generic

text: adult

text: Homo sapiens

text: NotI

text: SalI

text: melanoma (MeWo cell line)

text: 762 (synonym: hmel2)


text: The Gunma Univ. Institute for Molecular and Cellular Regulation

text: live

text: Generic

text: pancreatic beta cell

text: Mus musculus

text: Mus musculus betaHC9


text: Washington University School of Medicine

text: live

text: Generic

text: This C elegans library was made from fem-3(q23) worms (produce only sperm at 25 C). cDNA was generated via oligo (dT) priming and directionally cloned into pBluescript II SK+ vector with a modified polylinker. This library was subtracted with a second fem-1(hc17) (produce only oocytes at 25 C) library to enrich this library for sperm specific genes. First strand cDNA synthesis was primed with a NotI-15T oligo (sequence 5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'). After 2nd strand synthesis, XhoI-EcoRI adaptors (Stratagene #901120) were ligated, and cDNA was digested with NotI and ligated into XhoI-NotI digested vector.

characteristic: description

text: DH5alpha cells

text: Caenorhabditis elegans

text: XhoI

text: NotI

text: C elegans fem3 Q23 S1

text: phagemid

text: pBluescript II SK+


text: LYNX Therapeutics, Inc.

text: live

characteristic: INSDC status

text: Generic

text: monocyctic leukemia

text: THP-1 (TIB-202)

text: Cloning of PCR products from micro-beads carrying 3' end of up-regulated cDNA. THP-1 cells induced with 100 nM PMA in DMSO.

text: Homo sapiens


text: LYNX Therapeutics, Inc.

text: live

text: Generic

text: monocyctic leukemia

text: THP-1 (TIB-202)

text: Cloning of PCR products from micro-beads carrying 3' end of down-regulated cDNA. THP-1 cells non-induced (treated with DMSO only).

text: Homo sapiens


text: Stanford University

text: live

text: Generic

text: Illinois High Oil

characteristic: cultivar

text: Library was prepared by Statagene using the Uni-ZAP XR system (Stratagene BN937328-12). Clones were picked by a Q-bot after blue/white selection (ampicillin resistance - use 100 micrograms/microliter). Developed from a pool of equal amounts of RNA from developing embryos sampled at 14, 21, 28 and 35 days after pollination of the Illinois High Oil Maize Strain Cycle 90. This closed strain has been selected for high oil concentration for 90 generations and originates from the 1890s era open pollinated variety Burr's White

text: 14, 21, 28, and 35 days after pollination

text: E. coli SOLR

text: embryo

characteristic: organ

text: Zea mays

text: XhoI

text: EcoRI

text: embryo

characteristic: tissue-type

text: 687 - Early embryo from Delaware

text: plasmid

text: pBluescript SK


text: Kyushu University

text: live

text: Generic

characteristic: NCBI submission package

text: leukocyte

text: Cyprinus carpio

characteristic: organism

text: Cyprinus carpio leukocyte


text: Huashan Hospital, Shanghai Medical University

text: live

text: Generic

text: Homo sapiens

text: colon carcinoma

text: Homo sapiens colon carcinoma


text: Saitama University

text: live

text: Generic

text: Gallus gallus

text: brain

text: Gallus gallus brain


text: Simon Fraser University

text: live

text: Generic

text: Ailsa Craig

text: Plants, grown in MS solution for 6 weeks, were transferred into MS plus 170 mM NaCl solution for 24 hours. RNA was extracted from the roots and differential display-PCR was used to identify salt-induced or up-regulated cDNA.

text: six-week-old plants

text: Solanum lycopersicum

text: roots

text: Salt-stressed tomato roots


text: Capital University of Medical Sciences

text: live

text: Generic

text: nigro-striated neuron

characteristic: cell type

text: isolated from nigro cells treated with MPTP

text: Macaca mulatta

text: brain

text: Macaca mulatta brain


characteristic: External Id

text: Ludwig Institute for Cancer Research

text: live

text: Generic

text: A mini-library was made by cloning products derived from ORESTES PCR (U.S. Letters Patent application No. 196,716 - Ludwig Institute for Cancer Research) profiles into the pUC 18 vector. Reverse transcription of tissue mRNA and cDNA amplification were performed under low stringency conditions.

text: Adult

text: breast

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: Ludwig Institute for Cancer Research

text: live

text: Generic

characteristic: NCBI submission model

text: Adult

text: colon

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: Ludwig Institute for Cancer Research

text: live

text: Generic

text: Adult

characteristic: dev-stage

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: Ludwig Institute for Cancer Research

text: live

text: Generic

text: Adult

text: stomach

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: The Research Institute For Family Planning

text: live

text: Generic

text: adult

text: Rattus norvegicus

text: SD

characteristic: strain

text: testis

text: Rattus norvegicus testis SD adult


text: live

text: Generic

text: seven day

text: Rattus norvegicus

text: SD

text: testis

text: Rattus norvegicus testis SD seven day


text: Kyung Hee University, College of Dentistry

text: live

text: Generic

text: oral keratinocyte cells

text: KB(ATCC CCL-17)

characteristic: cell-line

text: Homo sapiens

text: PT-PCR products

text: pBluescript-T vector

characteristic: vector


text: Scottish Crop Research Institute

characteristic: INSDC center name

text: live

text: Generic

text: colchicine-doubled monoploid 851-5

text: cDNA library from shoot tissue of homozygous diploid potato. cDNA was synthesised with the superscript plasmid system (GibcoBRL) and directionally inserted into SalI (5' end)- NotI (3' end) digested pSPORT1.

text: E. coli DH10B

characteristic: lab-host

text: Solanum tuberosum

text: mixture of leaves and stems

text: potato shoot cDNA library

characteristic: title

text: plasmid


text: Michigan State University

characteristic: INSDC first public

characteristic: INSDC last update

characteristic: INSDC secondary accession

text: live

text: Generic

characteristic: SRA accession

text: Great Western

text: cDNAs were derived from reverse transcription of mRNA isolated from partially expanded leaves. The cDNA library was generated by ligating the cDNAs with the EcoRI site of pBluescript II KS (+) (Chiou and Bush, 1996. Plant Physiol. 110:511-520).

characteristic: description

text: vegetative stage

text: Beta vulgaris

text: EcoRI

text: EcoRV

text: partially expanded leaf

text: Sugar beet leaf cDNA library

text: plasmid

text: pBluescript II KS (+)


text: Medical Institute of Bioregulation, Kyushu University

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission package

text: skov

text: ovary

characteristic: organ

text: Homo sapiens

text: female

characteristic: sex

text: ovarian cancer

characteristic: tissue-type

text: human ovarian cancer cDNA


characteristic: External Id

text: Korea Research Institute of Bioscience and Biotechnology

text: live

text: Generic

characteristic: NCBI submission model

text: Intrathymic T-cell

characteristic: cell type

text: cDNA was made from total cytoplasmic RNA of sorted human intrathymic CD3-4-8- T-cell, adaptor ligated, amplified with PCR, and cloned into pGEM-T vector.

text: CD3-4-8- triple negative stage

characteristic: dev-stage

text: Homo sapiens

characteristic: organism

text: Thymus

text: KRIBB Human TN intrathymic T-cell cDNA library

text: Plasmid

text: pGEM-T


characteristic: INSDC center name

text: live

text: Generic

text: Intrathymic T-cell

text: cDNA was made from total cytoplasmic RNA of sorted human intrathymic CD3+4+8+ T-cell, adaptor ligated, amplified with PCR, and cloned into pGEM-T vector.

text: CD3+4+8+ double positive stage

text: Homo sapiens

text: Thymus

text: KRIBB Human DP intrathymic T-cell cDNA library

text: Plasmid

text: pGEM-T

characteristic: vector


characteristic: INSDC first public

text: live

text: Generic

text: Intrathymic T-cell

text: cDNA was made from total cytoplasmic RNA of sorted human intrathymic CD3+4+8- T-cell, adaptor ligated, amplified with PCR, and cloned into pGEM-T vector.

text: CD3+4+8- single positive stage

text: Homo sapiens

text: Thymus

text: KRIBB Human CD4 intrathymic T-cell cDNA library

characteristic: title

text: Plasmid

text: pGEM-T


text: University H.Poincare - Nancy 1

characteristic: INSDC last update

text: live

text: Generic

text: The cDNA library was prepared by suppressive subtractive hybridization of cDNAs isolated from cadmium-treated mycelium against cDNAs from untreated mycelium.

text: Paxillus involutus

text: ATCC 200175

characteristic: strain

text: Paxillus involutus cDNA, cadmium treated mycelium

text: Plasmid

text: pBS II SK


text: Stanford University

text: live

text: Generic

characteristic: cultivar

text: Directionally cloned, 14 day immature embryo library created with Stratagene's Zap Express cDNA protocol.

characteristic: description

text: 14 days after pollination

characteristic: lab-host

text: embryo

text: Zea mays

text: XhoI

text: EcoRI

text: embryo

text: 683 - 14 day immature embryo from Hake lab (HS)

text: phagemid

text: pBKCMV (Stratagene's Zap Express)


text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission package

text: melanoma cells

text: SK-Mel-28(ATCC HTB 72)

characteristic: cell-line

text: Homo sapiens

text: RT-PCR products human melanoma

text: pBluescript-T


characteristic: External Id

text: University of Mississippi Medical Center

text: live

text: Generic

text: Rattus norvegicus

text: hippocampal

characteristic: tissue-type

text: Rat hippocampal cDNA, differential display


text: IACR-Long Ashton Research Station

text: live

text: Generic

characteristic: NCBI submission model

text: Library constructed from cultures utilizing ammomium ions as a source of nitrogen

text: Zymoseptoria tritici

text: Strit

text: MgA Library

text: plasmid


text: Clemson University

text: live

text: Generic

text: Rice blast infection stage mRNA population at 48 hrs after inoculation was subtracted with mRNA population of healthy plant.

text: Infection stage, 48hrs, Subtraction with healthy plant

characteristic: dev-stage

text: Oryza sativa/Pyricularia oryzae mixed EST library

text: Subtracted Rice blast Infection Stage cDNA library

text: Plasmid

text: pBlueScriptII SK(+) Vector


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Library constructed by Life Technologies.

text: connective tissue

characteristic: organ

text: Homo sapiens

characteristic: organism

text: SalI

text: NotI

text: five pooled sarcomas, including myxoid liposarcoma, solitary fibrous tumor, malignant fibrous histiocytoma, gastrointestinal stromal tumor, and mesothelioma

text: phagemid (ampicillin resistant)


characteristic: INSDC center name

text: live

text: Generic

text: colon

text: Homo sapiens

text: SalI

text: NotI

text: juvenile granulosa tumor

text: phagemid (ampicillin resistant)

characteristic: vector


characteristic: INSDC first public

text: live

text: Generic

text: genitourinary tract

text: Homo sapiens

text: SalI

text: NotI

text: 2 pooled high-grade transitional cell tumors

characteristic: title

text: phagemid (ampicillin resistant)


characteristic: INSDC last update

text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Normalized to Cot >500. Average insert size 1.04kb. Normalized version of NCI_CGAP_Co18. Library constructed by Life Technologies.

text: colon

text: Homo sapiens

text: SalI

text: NotI

text: moderately differentiated adenocarcinoma

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: Cloned unidirectionally, no 5' adaptor. Primer: Oligo dT. Full-length library constructed by Life Technologies.

text: fetal, 14 wk post-conception

text: lung, cell line

text: Homo sapiens

text: EcoRV

text: NotI

text: male

characteristic: sex

text: phagemid (ampicillin resistant)


text: live

text: Generic

characteristic: description

text: whole blood

text: Homo sapiens

text: SalI

text: NotI

text: myeloid cells, 18 pooled CML cases, BCR/ABL rearrangement positive, includes both chronic phase and myeloid blast crisis

text: phagemid (ampicillin resistant)


text: live

text: Generic

characteristic: lab-host

text: kidney

text: Homo sapiens

text: SalI

text: NotI

text: 2 pooled Wilms' tumors, one primary and one metastatic to brain

text: phagemid (ampicillin resistant)


text: PUMC

text: live

characteristic: INSDC status

text: Generic

text: Rattus norvegicus

text: Wistar

characteristic: strain

text: Rattus norvegicus Wistar (Wang L)


text: PUMC

text: live

text: Generic

characteristic: NCBI submission package

text: Rattus norvegicus

text: Wistar

text: testis

text: Rattus norvegicus testis Wistar (Wang L)


characteristic: External Id

text: Anhui Medical University

text: live

text: Generic

text: adult worms

text: Schistosoma japonicum

text: mixed

text: Chinese

text: Lambda gt11 Express Library


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.26 kb. Library constructed by Life Technologies. Normalized versions of this library named NCI_CGAP_Co19 (Cot 50), NCI_CGAP_Co20 (Cot 500), and NCI_CGAP_Co21 (Cot >500).

text: colon

text: Homo sapiens

text: SalI

text: NotI

characteristic: tissue-type

text: phagemid (ampicillin resistant)


text: live

text: Generic

characteristic: NCBI submission model

text: Cloned unidirectionally. Primer: Oligo dT. Normalized to Cot 50. Average insert size 1.32kb. Normalized version of NCI_CGAP_Co18. Library constructed by Life Technologies.

text: colon

text: Homo sapiens

text: SalI

text: NotI

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Normalized to Cot 500. Average insert size 1.11kb. Normalized version of NCI_CGAP_Co18. Library constructed by Life Technologies.

text: colon

text: Homo sapiens

text: SalI

text: NotI

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: MYCN

characteristic: cell type

text: Homo sapiens

text: Neuroblastoma

text: NEUROBLASTOMA MYCN


text: Basic Medical Sciences, PUMC

text: live

text: Generic

text: Recombinant Bacterial

text: Ages 8-10 Weeks

characteristic: dev-stage

text: Mus musculus

text: Hermaphrodite

text: BALB/c

text: thymus

text: Mouse Plasmid pAP3neo Express Library