text: AgResearch Ltd

text: live

text: Generic

text: Isolated dendritic cells from T colubriformis infected adult Romney sheep. Normalised Library.

text: Ovis aries

text: dendritic cells

text: ONDC

text: plasmid


status: PUBLIC

characteristic: External Id

text: AgResearch Ltd

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Romney sheep pooled lymph node tissues from nodes draining sites infected with a variety of pathogens. Normalised Library.

characteristic: description

text: Ovis aries

characteristic: organism

text: lymph node tissues

tag: attribute

characteristic: tissue-type

text: ONLN

characteristic: title

text: plasmid


text: AgResearch Ltd

text: live

text: Generic

text: Romney Female Neonate Composite sample ovarian cortex

text: Ovis aries

text: ovarian cortex

text: OOCX

text: plasmid


text: AgResearch Ltd

text: live

text: Generic

text: Romney adult Peyers patches

text: Ovis aries

text: Peyers patches

text: OAPP

text: plasmid


text: AgResearch Ltd

text: live

text: Generic

text: Romney Growing wool follicles

text: Ovis aries

text: wool follicles

text: OSAA

text: plasmid


text: AgResearch Ltd

text: live

text: Generic

text: Wiltshire Growing wool follicles

text: Ovis aries

text: wool follicles

text: OSAB

text: plasmid


text: AgResearch Ltd

text: live

text: Generic

text: Wiltshire wool follicle in catagen phase

text: Ovis aries

text: wool follicle

text: OSCA

text: plasmid


text: AgResearch Ltd

text: live

text: Generic

text: Small intestine

text: Ovis aries

text: Small intestine

text: OSI

text: plasmid


text: AgResearch Ltd

text: live

text: Generic

text: Wiltshire wool follicle in proanagen phase

text: Ovis aries

text: wool follicle

text: OSPA

text: plasmid


text: AgResearch Ltd

text: live

text: Generic

text: Wiltshire wool follicles in telogen phase

text: Ovis aries

text: wool follicle

text: OSTA

text: plasmid


text: AgResearch Ltd

text: live

text: Generic

text: Romney Female Neonate Ovarian follicles (dissected from ovaries)

text: Ovis aries

text: ovary type 1 (primordial) follicles

text: plasmid


text: AgResearch Ltd

text: live

text: Generic

text: Ovis aries

text: ovary type 2 (primary) follicles

text: plasmid


text: AgResearch Ltd

text: live

text: Generic

text: Female Adult pool of ovine mammary gland tissues at various stages in the lactation cycle

text: Ovis aries

text: Mammary gland

text: OVMG

text: plasmid


text: AgResearch Ltd

text: live

text: Generic

text: Louse infected skin (Cockle)

text: Ovis aries

text: skin

text: plasmid


text: AgResearch Ltd

text: live

text: Generic

text: Orf infected skin

text: Ovis aries

text: skin

text: plasmid


text: AgResearch Ltd

text: live

text: Generic

text: Western Range Female Adult Composite sample lambda library generated from luteal tissue collected from 3 Western Range Ewes that were day 10 of the oestrous cycle

text: Ovis aries

text: corpus luteum

text: plasmid


text: AgResearch Ltd

text: live

text: Generic

text: Western Range Ewe-cross composite sample day 15 embryo lambda library

text: Ovis aries

text: whole conceptus

text: plasmid


text: AgResearch Ltd

text: live

text: Generic

text: Ovine fetal lymphoid tissue

text: Ovis aries

text: lymphoid tissue

text: OFLT

text: plasmid


text: AgResearch Ltd

text: live

text: Generic

text: foetal liver tissue pooled from d 31/35,d45,d90,d135 embryos

text: Ovis aries

text: liver

text: OFLV

text: plasmid


text: AgResearch Ltd

text: live

text: Generic

text: Muscle tissues from hind limb of d 45 embryo (myoblasts)

text: Ovis aries

text: Muscle

text: plasmid


text: ARC Seibersdorf Research GesmbH

text: live

text: Generic

text: INA 100

characteristic: cultivar

text: Smart cDNA library construction kit; Clontech: PT3000-1

text: Root

characteristic: organ

text: Ipomoea batatas

text: Sfi A

text: Sfi B

text: small thin, white roots before white to orange colour change

text: Sweetpotato cDNA library A

text: Phage vector

characteristic: vector


text: ARC Seibersdorf Research GesmbH

text: live

text: Generic

text: Zapallo and SPK004

text: Root

text: Ipomoea batatas

text: Sfi A

text: Sfi B

text: white, thin/pencil sized roots, before storage root development

text: Sweetpotato cDNA library B

text: Phage vector


text: ARC Seibersdorf Research GesmbH

text: live

text: Generic

text: Zapallo and SPK004

text: Root

text: Ipomoea batatas

text: Sfi A

text: Sfi B

text: medium to big, orange/purple coloured storage roots

text: Sweetpotato cDNA library C

text: Phage vector


text: ARC Seibersdorf Research GesmbH

text: live

text: Generic

text: Feng Shu Bai

text: Root

text: Ipomoea batatas

text: Sfi A

text: Sfi B

text: Sweetpotato cDNA library D

text: Phage vector


text: ARC Seibersdorf Research GesmbH

text: live

text: Generic

text: Wagabolige and Feng Shu Bai

text: Lambda

characteristic: lab-host

text: Root

text: Ipomoea batatas

text: Sfi A

text: Sfi B

text: white, medium to big storage roots

text: Sweetpotato cDNA library E

text: Phage vector


text: Sun Yat-sen University

text: live

text: Generic

text: Xianglian

text: Mid-maturation stage

characteristic: dev-stage

text: E.coli BM25.8

text: Seed

text: Nelumbo nucifera

text: Embryonic axes

text: Nelumbo nucifera embryonic axis cDNA library


text: Technische Universitaet Muenchen

text: live

text: Generic

text: Bettina

text: cDNA library highly enriched for Phytophthora infestans induced genes derived from the moderately resistant potato cv. Bettina 24 hpi.

text: leaf

text: Solanum tuberosum


text: Technische Universitaet Muenchen

text: live

text: Generic

text: Bettina

text: cDNA library highly enriched for Phytophthora infestans induced genes derived from the moderately resistant potato cv. Bettina 72 hpi.

text: leaf

text: Solanum tuberosum


text: Technische Universitaet Muenchen

text: live

text: Generic

text: Bettina

text: cDNA library highly enriched for Phytophthora infestans repressed genes derived from the moderately resistant potato cv. Bettina 72 hpi.

text: leaf

text: Solanum tuberosum


text: Friedrich-Alexander University Erlangen-Nurnberg

text: live

text: Generic

text: Solara

text: sprouting tuber buds were excised from potato tubers, PolyA-RNA was isolated and used to create a cDNA-library by means of by means of the 'SMART cDNA library construction Kit' (CLONTECH). Normalisation was performed essentially as described by Ko (1990, Nucl. Acid Res. 16, 9877) with some modification of Kohchi et al. (1995, Plant J. 8, 771-776). Normalised cDNAs were digested with NotI and cloned into NotI digested pCRblunt vector. Sequence submission managed by GABI Primary Database at RZPD: http://gabi.rzpd.de

text: potato tubers after ca. 3 months of storage, sprouting buds (ca. 2mm length)

text: E. coli XL1-blue

text: Solanum tuberosum

text: NotI (GCGGCCGC)

text: NotI (GCGGCCGC)

text: potato tuber

text: SSBN

text: pCR-Blunt (Invitrogen)


text: live

text: Generic

text: Solara

text: sprouting tuber buds were excised, PolyA-RNA was isolated and used to create a cDNA-library by means of the SMART cDNA library construction Kit (CLONTECH). cDNA Fragments were inserted into the vector Lambda TripleEx Sfi I. Subsequently, plasmids were obtained by in vivo excision according to manufacturers instruction. Sequence submission managed by GABI Primary Database at RZPD: http://gabi.rzpd.de

text: E. coli BM25.8

text: Solanum tuberosum

text: Sfi IA

text: Sfi IB

text: potato tuber

text: SSBT


text: live

text: Generic

text: Solara

text: dormant tuber buds were excised, PolyA-RNA was isolated and used to create a cDNA-library using the Time Saver(TM) cDNA-Synthesis Kit (Amersham) and inserted into the vector Lambda ZAPII vector (Stratagene). Average insert size is 750bp Sequence submission managed by GABI Primary Database at RZPD: http://gabi.rzpd.de

text: potato tuber, dormant buds

text: XL1-Blue MRF

text: Solanum tuberosum

text: EcoRI/NotI

text: EcoRI/NotI

text: potato tuber

text: STDB

text: pBluescript SK-


text: live

text: Generic

text: Solara

text: dormant tuber buds were excised, PolyA-RNA was isolated and used to create a cDNA-library by means of the SMART cDNA library construction Kit (CLONTECH). CDNA Fragments were inserted into the vector Lambda TripleEx Sfi I. Subsequently, plasmids were obtained by in vivo excision according to manufacturers instruction. Sequence submission managed by GABI Primary Database at RZPD: http://gabi.rzpd.de

text: potato tuber, dormant buds

text: E. coli BM25.8

text: Solanum tuberosum

text: Sfi IA

text: Sfi IB

text: potato tuber

text: SDBT


text: live

text: Generic

text: Solara

text: potato tuber, dormant buds (taken from freshly harvested tubers)

text: E. coli XL1-blue

text: Solanum tuberosum

text: NotI (GCGGCCGC)

text: NotI (GCGGCCGC)

text: potato tuber

text: SDBN

text: pCR-Blunt (Invitrogen)


text: Pasteur Institute

text: live

text: Generic

text: ookinete

text: Plasmodium berghei

text: Plasmodium berghei ookinete stage


text: University of Aberdeen

text: live

text: Generic

text: country: United Kingdom:Scotland

text: Parr

text: Salmo salar

text: liver

text: Salmo salar liver Parr


text: Chiangmai University

text: live

text: Generic

text: 1-6 weeks

text: Gallus gallus

text: male/female

characteristic: sex

text: Thai chicken

characteristic: strain

text: ESTs derived from chicken muscle by DDRT-PCR


characteristic: External Id

text: Kochi University

characteristic: INSDC center name

text: live

text: Generic

text: Lactuca sativa

text: Lactuca sativa mRNA (Kiba A)


text: Sabanci University

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Phalaris canariensis

text: Leaf

text: Canary seed leaf tissue


text: IEW

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: SSH cadmium enriched library

text: Orchesella cincta

text: mixed

text: lab culture


text: RIKEN

text: live

text: Generic

characteristic: NCBI submission package

text: isolation_source: fungus comb of Macrotermes gilvus; environmental_sample

characteristic: description

text: uncultured fungus

text: Subtractive cDNA library from fungus comb


text: RIKEN

text: live

text: Generic

text: uncultured fungus

characteristic: organism

text: Non-subtractive cDNA library from fungus comb


text: National Centre for Crop Moleculer Design

text: live

text: Generic

text: Tapidor

text: Brassica napus

text: Leaves and Siliques

characteristic: tissue-type

text: Brassica napus Full-length cDNA Library

characteristic: title

text: Phagemid

text: Lambda ZAP II


text: The Second Affiliated Hospital, Sun Yat-Sen University

text: live

text: Generic

text: ESTs expressed preferentially in mycelial form of Penicillium marneffei.

text: Talaromyces marneffei

text: Suppression subtraction hybridization cDNA library of mycelial form of Penicillium marneffei


text: Institute of Hydrobiology, Chinese Academy of Sciences

text: live

text: Generic

text: Epinephelus coioides

text: male

text: pituitary

text: orange-spotted grouper pituitary cDNA library


text: McGill University

text: live

text: Generic

text: SSH cDNA library was prepared from RNA of Rhizoctonia solani AG3 on a minimum media with cellulose as tester, and RNA of Rhizoctonia solani AG3 during interaction with potato sprout as driver. The cDNA isolated with SSH were cloned in the vector TOPO-PcR2.1. The inserts were amplified by PCR and the corresponding amplicons were printed on microarray slides. The down-regulated genes were identified by microarray.

characteristic: isolate

text: Rhizoctonia solani AG-3

text: Rhizoctonia solani AG3 SSH down-regulated cDNA library during interaction with Solanum tuberosum

text: plasmid

text: TOPO pcr2.1


text: Altadis - Institut du Tabac

text: live

text: Generic

characteristic: cultivar

text: 2 w after germination

text: Nicotiana tabacum

text: Seedling


text: The Jackson Laboratory

text: live

text: Generic

text: oocyte

characteristic: cell type

text: Cloned unidirectionally from mRNA prepared from fully-grown germinal vesicle stage oocytes isolated 46 hours after PMSG injection. NotI(dT) primer: 5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'. SalI linker: 5'-TCGACCCACGCGTCCG-3'. cDNAs were size-selected for 500 bp and cloned into the SalI/NotI sites of pSPORT vector (Life Technologies). Average insert size: 1.5 kb. I.M.A.G.E. library ID 1182.

characteristic: lab-host

text: Mus musculus

text: SalI (5')

text: NotI (3')

text: female

text: Eppig Hampl Solter fully-grown mouse oocyte

text: phagemid

characteristic: vector


text: Altadis - Institut du Tabac

text: live

text: Generic

text: before topping

characteristic: dev-stage

text: Nicotiana tabacum

text: Leaf (lamina)


characteristic: External Id

text: Altadis - Institut du Tabac

text: live

text: Generic

text: Burley 21

text: Maturity

text: Nicotiana tabacum

text: Leaf (lamina)


text: Advanced Technologies (Cambridge) Ltd

characteristic: INSDC center name

text: live

text: Generic

text: Maturity

text: Nicotiana tabacum

text: Leaf (lamina)


text: Altadis - Institut du Tabac

characteristic: INSDC first public

text: live

text: Generic

text: Before topping + 1w after topping + maturity

text: Nicotiana tabacum

text: Leaf (midrib)


text: Altadis - Institut du Tabac

characteristic: INSDC last update

text: live

text: Generic

text: Before topping + 1w after topping + Maturity

text: Nicotiana tabacum

text: Leaf (stem)


text: live

characteristic: INSDC status

text: Generic

text: Funding sources: Award 27741 from The Research Council of Norway; P. S. Soltis and D. E. Soltis laboratory (University of Florida); Floral Genome Project (NSF Plant Genome Research Program, project DBI-0115684); The Natural History Museum, University of Oslo, Norway; Tissue collection locality: Mauna Kea, Hawaii, United States; Treatment: field-frozen in liqN; Primers: M13F and M13 R; Primary titer: 1,5 x10^6, Amplified titer: 2,4 x10^6. The sequence provided is trimmed of vector and low quality regions. Full sequence and original trace file are available from the Plant Genome Network website (http://pgn.cornell.edu)

text: SOLR

text: Stenogyne rugosa

text: EcoRI

text: XhoI

text: developing fruits

text: pBluescript


text: Sun Yat-sen University

text: live

text: Generic

characteristic: NCBI submission model

text: Shanyou 523

text: Mid-maturation stage

text: E.coli BM25.8

text: Seed

characteristic: organ

text: Arachis hypogaea

text: Cotyledons

text: Shanyou 523 Peanut Cotyledon cDNA Library


text: c/o UniMi DiProVe, Parco Tecnologico Padano

text: live

text: Generic

characteristic: NCBI submission package

text: Bolero

text: country: Italy

characteristic: description

text: mature fruit

text: Prunus persica

text: fruit skin

text: Prunus persica fruit skin mature fruit


text: Universidad de Cordoba

text: live

text: Generic

text: Camarosa

text: Fragaria x ananassa

characteristic: organism

text: crown

characteristic: tissue-type

text: Fragaria x ananassa Camarosa crown


text: Universidad de Cordoba

text: live

text: Generic

text: Andana

text: Fragaria x ananassa

text: crown

text: Fragaria x ananassa Andana crown

characteristic: title


text: Universidad Publica de Navarra

text: live

text: Generic

text: country: Spain

text: Pleurotus sp. 'Florida'

text: Lamellae

text: Pleurotus sp. 'Florida' Lamellae


text: Universidad Publica de Navarra

text: live

text: Generic

text: country: Spain

text: Pleurotus sp. 'Florida'

characteristic: strain

text: Lamellae

text: Pleurotus sp. 'Florida' Lamellae N001


text: Rubber Research Institute of India

text: live

text: Generic

text: RRII 105

characteristic: cultivar

text: The clones are PCR amplified fragments being cloned into PCR-TRAP vector of GenHunter Corporation, USA

text: Hevea brasiliensis

text: Hind III

text: Hind III

text: bark

text: Hevea DD RT-PCR TPD Specific library

text: Plasmid

text: PCR-TRAP Vector


text: Children's Memorial Research Center

text: live

text: Generic

text: UI-EH-IJ0 is a subtracted library derived from UI-EH-HG1. The library was constructed and subtracted according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is GAAGGCTAGT. Tissue was obtained from the Provasoli-Guillard National Center for Culture of Marine Phytoplankton (CCMP).

text: DH10B (Life Technologies) (T1 phage resistant)

characteristic: lab-host

text: Emiliania huxleyi

text: EcoR I

text: Not I

text: CCMP 371

text: Plasmid

text: pT7T3-Pac (Pharmacia) with a modified polylinker


text: Cotton Research Institute of the Chinese Academy of Agricultural Sciences

text: live

text: Generic

text: EcoRI and MseI, cDNA-AFLP technique. Gossypium hirsutum plant male sterile and fertile anthers were collected after identification by the microscope in laboratory.RNA was extracted, and double-stranded cDNA was synthesized frome total RNA. The cDNA was digested with MseI and MseI, adapters were ligated to the termini, and two rounds of PCR were performed. On the first round the cDNAs were amplified with primers specific for the adapters. The products of this first round were amplified with primers based on the adapters containing two additional bases at the 3' end.

text: anther

text: Gossypium hirsutum

text: MseI

text: MseI

text: Plasmid

characteristic: vector


text: Universite Libre de Bruxelles

text: live

text: Generic

text: Bright Yellow 2 (BY-2)

characteristic: cell-line

text: Bright Yellow 2

text: Nicotiana tabacum

text: Tobacco BY-2 ESTs modulated by Rhodococcus fascians


characteristic: External Id

text: Iowa State University

text: live

text: Generic

text: mRNA from LCM-dissected maize inbred line Mo17 shoot apical meristems was amplified with a T7 RNA polymerase-based linear RNA amplification method (Nakazono et al., 2003, Plant Cell 15:583-596). The amplified RNA was converted to cDNA and the cDNA was sequenced at 454 Life Sciences.

text: Zea mays

text: shoot apical meristem

text: LCM-dissected maize inbred line Mo17 shoot apical meristem cDNA


text: The Georgia Institute of Technology

characteristic: INSDC center name

text: live

text: Generic

text: Directional cDNA library

text: 4th and 5th larval instar

characteristic: dev-stage

text: Vespula squamosa

text: Female

characteristic: sex

text: Whole body

text: Caste=Worker, Stage=4th and 5th larval instar

text: Plasmid


text: The Georgia Institute of Technology

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Directional cDNA library

text: Pupa

text: Vespula squamosa

text: Male

text: Whole body

text: Caste=Male, Stage=Pupa

text: Plasmid


text: The Georgia Institute of Technology

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Directional cDNA library

text: Pupa

text: Vespula squamosa

text: Female

text: Whole body

text: Caste=Worker, Stage=Pupa

text: Plasmid


text: The Georgia Institute of Technology

text: live

text: Generic

text: Directional cDNA library

characteristic: description

text: Imago

text: Vespula squamosa

characteristic: organism

text: Female

text: Whole body

text: Caste=Worker, Stage=Adult

text: Plasmid


text: The Georgia Institute of Technology

text: live

text: Generic

text: Directional cDNA library

text: 1st, 2nd, and 3rd larval instar

text: Vespula squamosa

text: Female

text: Whole body

characteristic: tissue-type

text: Caste=Queen, Stage=1st, 2nd, and 3rd larval instar

characteristic: title

text: Plasmid


text: The Georgia Institute of Technology

text: live

text: Generic

text: Directional cDNA library

text: 1st, 2nd, and 3rd larval instar

text: Vespula squamosa

text: Female

text: Whole body

text: Caste=Worker, Stage=1st, 2nd, and 3rd larval instar

text: Plasmid


text: The Georgia Institute of Technology

text: live

text: Generic

text: Directional cDNA library

text: 4th and 5th larval instar

text: Vespula squamosa

text: Female

text: Whole body

text: Caste=Queen, Stage=4th and 5th larval instar

text: Plasmid


text: The Georgia Institute of Technology

text: live

text: Generic

text: Directional cDNA library

text: Imago

text: Vespula squamosa

text: Male

text: Whole body

text: Caste=Male, Stage=Adult

text: Plasmid


text: The Georgia Institute of Technology

text: live

text: Generic

text: Directional cDNA library

text: Imago

text: Vespula squamosa

text: Female

text: Whole body

text: Caste=Queen, Stage=Adult

text: Plasmid


text: The Georgia Institute of Technology

text: live

text: Generic

text: Directional cDNA library

text: Pupa

text: Vespula squamosa

text: Female

text: Whole body

text: Caste=Queen, Stage=Pupa

text: Plasmid


text: The Georgia Institute of Technology

text: live

text: Generic

text: Directional cDNA library

text: Egg

text: Vespula squamosa

text: Female

text: Whole body

text: Caste=Queen, Stage=Egg

text: Plasmid


text: Rubber Research Institute of India

text: live

text: Generic

text: RRIM 600

characteristic: cultivar

text: RNA was isolated from leaves of one year old plants stressed for 28 days and control (watered alternate days to maintain field capacity) and subjected to DDRT-PCR analyses using GenHunter's differential display kit following the manufacture's protocol. The clones are PCR amplified fragments being cloned into PCR-TRAP vector of GenHunter Corporation, USA

text: Hevea brasiliensis

text: Hind III

text: Hind III

text: LEAF

text: Hevea cv. RRIM 600 DDRT-PCR drought responsive transcript library

text: Plasmid

text: PCR-TRAP Vector


text: Rubber Research Institute of India

text: live

text: Generic

text: RRII 105

text: Hevea brasiliensis

text: Hind III

text: Hind III

text: LEAF

text: Hevea cv. RRII 105 DDRT-PCR drought responsive transcript library

text: Plasmid

text: PCR-TRAP Vector


text: McGill University

text: live

text: Generic

text: kennebec

text: SSH cDNA library was prepared from RNA of solanum tuberosum and Rhizoctonia solani AG3 in interaction as tester, and RNA of Rhizoctonia solani AG3 growing alone and not infected potato sprout as driver. The cDNA isolated with SSH were cloned in the vector TOPO-PcR2.1. The inserts were amplified by PCR and the corresponding amplicons were printed on microarray slides. The up-regulated genes were identified by microarray.

text: Sprout

characteristic: organ

text: Solanum tuberosum

text: solanum tuberosum SSH up-regulated cDNA library during interaction with rhizoctonia solani AG3

text: plasmid

text: TOPO pcr2.1


text: NI Vavilov Institute of General Genetics, Russian Academy of Sciences

text: live

text: Generic

text: A Sequence-Indexed Library of Insertion Mutations in the Arabidopsis Genome. mRNA (EST) was obtained from transgenic line with artificial constitutive expression of 1st exon of At1g12860 gene.

text: 4 weeks old plant

text: W-line

characteristic: isolate

text: leaf

text: Arabidopsis thaliana

text: hermaphrodite

text: Arabidopsis thaliana TDNA insertion lines PCR

text: overexpression vector

text: pFE12860OE(GenBank Accession Number: DQ995484)

characteristic: vector


characteristic: External Id

text: Medical University of South Carolina

text: live

text: Generic

text: PBL

characteristic: cell type

text: Tursiops truncatus

text: PBL

text: Plasmid


text: Universidad Publica de Navarra

characteristic: INSDC center name

text: live

text: Generic

text: country: Spain

text: Pleurotus sp. 'Florida'

characteristic: strain

text: Pleurotus sp. 'Florida' N001


text: British Antarctic Survey

characteristic: INSDC first public

text: live

text: Generic

text: Subtractive library

text: Smolt

characteristic: dev-stage

text: Gill

text: Salmo salar

text: Gill

text: Gill SSH library

text: pGEM-T


text: British Antarctic Survey

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Subtractive library

text: Smolt

text: Kidney

text: Salmo salar

text: Kidney

text: Kidney SSH library

text: pGEM-T


text: British Antarctic Survey

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Subtractive library

text: Smolt

text: Pituitary

text: Salmo salar

text: Pituitary

text: Pituitary SSH library

text: pGEM-T


text: British Antarctic Survey

text: live

text: Generic

text: Subtractive library

characteristic: description

text: Smolt

text: Brain

text: Salmo salar

characteristic: organism

text: Brain

text: Brain SSH library

text: pGEM-T


text: Oak Ridge National Laboratory

text: live

text: Generic

text: mRNA from roots, stems and leaves of Populus hybrid H11-11 up-regulated in response to auxin treatment

text: Populus trichocarpa x Populus deltoides

text: roots, stems and leaves

characteristic: tissue-type

characteristic: title


text: Oak Ridge National Laboratory

text: live

text: Generic

characteristic: cultivar

text: mRNA from roots, stems and leaves of Populus hybrid H11-11 down-regulated in response to auxin treatment

text: Populus trichocarpa x Populus deltoides

text: roots, stems and leaves


text: Laval University

text: live

text: Generic

text: Suppression Subtractive Hybridization cDNA library. Tester: perithecia cDNA population; Driver: mycelium cDNA population.

text: Sexual fruit body

text: Escherichia coli (Invitrogen's TOP10 cells)

characteristic: lab-host

text: Ophiostoma novo-ulmi subsp. novo-ulmi

text: PCR product cloning through TA-b

text: PCR product cloning through TA-b

text: Perithecia (sexual fruit bodies)

text: Onu-per

text: Plasmid


text: Laval University

text: live

text: Generic

text: Suppression Subtractive Hybridization cDNA library. Tester: synnemata cDNA population; Driver: mycelium cDNA population.

text: Asexual fruit body

text: Escherichia coli (Qiagen EZ competent cells)

text: Ophiostoma novo-ulmi subsp. novo-ulmi

text: PCR product cloning through UA-b

text: PCR product cloning through UA-b

text: Synnemata (asexual fruit bodies)

text: Onu-syn

text: Plasmid

text: pDrive

characteristic: vector


characteristic: External Id

text: Laval University

text: live

text: Generic

text: Suppression Subtractive Hybridization cDNA Library. Tester: cDNA population from mycelium grown at 15C; Driver: cDNA population from mycelium grown at 22 to 24C.

text: Ophiostoma novo-ulmi subsp. novo-ulmi

text: PCR product cloning through UA-b

text: PCR product cloning through UA-b

text: Mycelium

text: Plasmid

text: pDrive


text: Laval University

text: live

text: Generic

text: Suppression Subtractive Hybridization cDNA library. Tester: cDNA population from mycelium grown at 31C; Driver: cDNA population from mycelium grown at 22 to 24C.

text: Escherichia coli (Invitrogen's TOP10 competent cells)

text: Ophiostoma novo-ulmi subsp. novo-ulmi

text: PCR product cloning through TA-b

text: PCR product cloning through TA-b

text: Mycelium

text: Plasmid


text: School of Biotechnology, Kangwon National University

text: live

text: Generic

text: EST obtained by using differential display RT-PCR

text: leaf

text: Nicotiana glauca x Nicotiana langsdorffii leaf DDRT-PCR


text: live

text: Generic

text: EST obtained by using differential display RT-PCR; from tumore produced on Nicotiana glauca x Nicotiana langsdorffii

text: tumor

text: Nicotiana glauca x Nicotiana langsdorffii tumor DDRT-PCR


text: live

text: Generic

text: Nicotiana glauca

text: leaf

text: Nicotiana glauca leaf DDRT-PCR


text: live

text: Generic

text: Nicotiana langsdorffii

text: leaf

text: Nicotiana langsdorffii leaf DDRT-PCR


text: College of Forest Resources and Environment, Nanjing Forestry University

text: live

text: Generic

text: nature mutant

text: We constructed a forward and a reverse subtractive cDNA libraries using SSH (suppression subtractive hybridization), the mutant as the tester and the wide type as the driver.

text: Escherichia coli DH5alpha

text: xylem

characteristic: organ

text: Cunninghamia lanceolata

text: EcoRI±

text: NotI

text: Chinese fir differentiating xylem specifically expressed cDNA library

text: Plasmid

text: pGEM T-easy Vector


text: National Institute of Advanced Industrial Science and Technology

characteristic: INSDC center name

text: live

text: Generic

characteristic: cell-line

text: Xanthi

text: Nicotiana tabacum

text: Nicotiana tabacum XD6S suspension-cultured cells


text: National Cancer Institute / NIH

characteristic: INSDC first public

text: live

text: Generic

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer 5'-GACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3', double-stranded cDNA was cloned into the NotI and EcoRV sites of pExpress-1 (EcoRV site destroyed upon ligation). Library was size-selected for an average insert size of 1.7 kb. Primary library, non-amplified. Use M13(-21) to generate 3' end reads and M13R to generate 5' end reads. Library was constructed by Open Biosystems (Huntsville, AL). Note: this is a (http://zgc.nci.nih.gov/) Zebrafish Gene Collection library.

text: DH10B TonA

text: ovary

text: Danio rerio

text: EcoRV

text: NotI

text: pooled ovaries, including ooctyes and somatic tissue

text: plasmid


text: National Cancer Institute / NIH

text: live

text: Generic

text: DH10B TonA

text: egg

text: Danio rerio

text: EcoRV

text: NotI

text: activated eggs, 10-15 min, pooled

text: plasmid


text: C.R.A.-Istituto Sperimentale per l'Agrumicoltura; Parco Scientifico e Tecnologico della Sicilia S.c.p.a. (PSTS S.c.p.a)

text: live

text: Generic

text: Moro nucellare 58-8D-I

text: Vector: pGEM-T; Total RNA was isolated from Moro (tester) and Cadenera (driver) flesh orange. Subtractive cDNA library was constructed using PCR-Select cDNA subtraction Kit (Clontech) to isolate differential expressed genes at maturity time.

text: maturity

characteristic: dev-stage

text: Citrus sinensis

text: EcoRI

text: EcoRI

text: flesh

text: Citrus sinensis flesh suppression subtractive hybridization (SSH) cDNA library Moro nucellare 58-8D-I cultivar

text: Plasmid

text: PGEM Teasy vector


characteristic: INSDC last update

text: live

text: Generic

text: Biondo cadenera

text: maturity

text: Citrus sinensis

text: EcoRI

text: EcoRI

text: flesh

text: Citrus sinensis flesh suppression subtractive hybridization (SSH) cDNA library Blonde cadenera cultivar

text: Plasmid

text: PGEM Teasy vector


text: Biochemistry Department, Chemistry Institute, Sao Paulo University

text: live

characteristic: INSDC status

text: Generic

text: larvae

text: Spodoptera frugiperda

text: EcoRI

text: EcoRI

text: midgut

text: S. frugiperda midgut cDNA library

text: Phage

text: Lamba Zap II


text: live

text: Generic

characteristic: NCBI submission model

text: larvae

text: midgut

text: Tenebrio molitor

text: EcoRI

text: EcoRI

text: T. molitor midgut cDNA library

text: Phage

text: Lamba Zap II


text: Sun Yat-Sen University

text: live

text: Generic

characteristic: NCBI submission package

text: Ceriops tagal seedlings with 2~4 leaves was treated with 3% artificial seawater(>500mM NaCl) for 0.5h,2h,4h. Equivalent pool of total RNA from the three treatments was used as Tester. The untreated control was used as Driver. Driver was subtracted from Tester and the EST in this library are theoretically up-regulated in transcription level under high salinity in Ceriops tagal leaf.

text: seedling

text: Ceriops tagal

text: leaf

text: Ceriops tagal supressive subtracted library


text: Faculty of Science & Technology

text: live

text: Generic

text: Appressoria

text: Colletotrichum gloeosporioides

text: Appressoria


text: Faculty of Science & Technology

text: live

text: Generic

text: Germinating conidia

text: Colletotrichum gloeosporioides

text: Germinating conidia


text: Washington State University

text: live

text: Generic

text: Mature uredinispores

text: Wheat

text: Puccinia striiformis f. sp. tritici

text: Sfi I

text: Sfi I

characteristic: strain

text: Urediniospores

text: Puccinia striiformis f. sp. tritici. Full-length cDNA library

text: Plasmid

text: pDNR-LIB


text: The University of Birmingham

text: live

text: Generic

text: Stickleback treated with compounds (oestradiol, methyltestosterone, dibenzanthracene, hexabromocyclododecane) subtracted versus solvent controls

characteristic: description

text: Immature

text: Liver

text: Gasterosteus aculeatus

characteristic: organism

text: male

characteristic: sex

text: Forward SSH Library (FSH)

text: pCR2.1 (Invitrogen)


text: USDA - ARS

text: live

text: Generic

text: A high quality EST with at least 200 contiguous bases at Trace Tuner score of 20 or better. Tissue dissections by J. Habibi (University Missouri-Columbia). Library construction and sequencing by P.M. Dang (USDA-ARS-USHRL).

text: Adults

text: Field collected from citrus, Riverside, CA (2004)

characteristic: isolate

text: Homalodisca vitripennis

text: EcoRI

text: XhoI

text: Mixed population

text: Midgut

characteristic: tissue-type

text: Homalodisca vitripennis (syn. H. coagulata) midgut

characteristic: title

text: pBluescript II SK+


characteristic: External Id

text: USDA - ARS

text: live

text: Generic

text: Adults

characteristic: lab-host

text: Homalodisca vitripennis

text: EcoRI

text: XhoI

text: Mixed population

text: Salivary gland

text: Homalodisca vitripennis (syn. H. coagulata) salivary gland

text: pBluescript II SK+

characteristic: vector


text: University of Veterinary Medicine Hannover

characteristic: INSDC center name

text: live

text: Generic

text: The subtracted library contains ESTs from hypobiosis-induced (L3i) and non-induced (L3ni) third stage lungworm larvae. Subtraction was achieved using the Subtractive Suppression Hybridization (SSH) technique. Differentially expressed sequences were verified by differential screening and cDNA dot blot using freshly isolated mRNA of the two larval populations.

text: third-stage larvae

text: Bos taurus

text: Dictyocaulus viviparus

text: no differentiation

text: D. viviparus subtracted L3i/L3ni library

text: Plasmid


text: USDA-ARS FRRL

characteristic: INSDC first public

text: live

text: Generic

text: The library was constructed and normalized as described by Bonaldo, M.F., Lennon, G. and Soares, M.B. (1996), Genome Research 6(9): 791-806. An identiying tag was added at the 3'during cDNA synthesis: insertAAAAAAAAAAAAAAAAAATGCGA (for rhizome clones) or insertAAAAAAAAAAAAAAAAAATCGGA (for spring tiller clones).

text: Leymus cinereus x Leymus triticoides

text: EcoRI (5' side of insert)

text: NotI (3' side of insert)

text: Rhizome and Spring tiller

text: BG01 - normalized library

text: Plasmid

text: pBS II SK(+)


text: University of Leeds

text: live

text: Generic

text: Primula vulgaris

text: Primula vulgaris library (Li J)


text: National Research Institute for Family Planning

text: live

text: Generic

text: peri-implantation and early-sectory

characteristic: dev-stage

text: Homo sapiens

text: Female

text: endometrium

text: Human endometrium substracted library


text: The National Forestry University of Ukraine, Department of Forestry

characteristic: INSDC last update

text: live

text: Generic

text: The library was made from the roots of 7 days old seedlings. Double-stranded cDNA was prepared from 5 micrograms of mRNA and directionally ligated into the ZAP Express vector using the ZAP Express cDNA Gigapack III Gold Cloning Kit according to manufacturer's instructions with modifications (Stratagene) and excised to form a pBK-CMV phagemid vector.

text: 7 days old seedlings

text: root

characteristic: organ

text: Pinus sylvestris

text: Xho 1

text: Hermaphrodite

text: Various genotypes

text: Pine Lambda Zap Express seedling roots Library

text: ZAP Express

text: pBK-CMV vector


text: The Institute for Genomic Research (TIGR)

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Arabidopsis roots, inflorescence, callus, young seedlings and Arabidopsis treated with cold, heat, salt, 2,4-D, hydrogen peroxide, UV, IAA, Xanthomonas and Pseudomonas.

text: Arabidopsis thaliana

text: Columbia

text: see description

text: pooled cDNA populations


text: Ohio State University

text: live

text: Generic

characteristic: NCBI submission package

text: Rutgers

characteristic: cultivar

text: cDNA library was constructed by using the Clontech PCR-Select cDNA subtraction Kit (Clontech, Cat. # K1804-1). Partial cDNA fragments were shotgun-cloned into pCR2.1-TOPO vector (Invitrogen, Cat. #K4550-40) and sequenced using M13R primer.

text: Developmental stage

text: Leaf

text: Solanum lycopersicum

text: Tomato expression library upon Potato spindle tuber viroid

text: Plasimd

text: TOPO TA cloning vector pTA2.1 (Invitrogen)


text: ValiGen

text: live

text: Generic

text: Solanum lycopersicum

characteristic: organism

text: Fruit

text: Tomato fruit library


text: Scottish Agricultural College

text: live

text: Generic

text: Moneymaker

text: RNA was extracted from leaf tissue of colonised and non colonised Glomus mosseae (BEG 12) tomato plants. Differential display - PCR was used to identify differentially expressed cDNAs.

characteristic: description

text: 12 week old plants

text: Solanum lycopersicum

text: Leaf

text: Tomato Leaf


text: Murdoch University

text: live

text: Generic

text: Templates for the DDRT-PCR were reverse-transcribed from mRNA extracted (1) from direct extracted giant cell cytoplasmic contents and (2) from healthy root tissue cultured and harvested in parallel on a separate plate. Transcripts profiles were compared between (1) and (2). cDNAs with differential expression were direct sequenced using the same arbitrary or anchor primer which was used to generate those DD bands.

text: In-vitro cultures of vegetative plants on media plates, 25 days post-infection

text: Solanum lycopersicum

text: Cytoplasmic contents extracted from individual giant cells in nematode feeding site

text: Direct sequenced cDNAs of tomato generated by mRNA differential-display RT-PCR


text: Purdue University

text: live

text: Generic

text: cDNAs made from fruit mRNAs of a thick-juice breeding line (70620) were subtracted by cDNAs made from fruit mRNAs of a thin-juice breeding line (70320) according to the manufacture's protocol of PCR-select cDNA subtraction protocol (Clonetech manual #PT1117-1). The resultant PCR amplified products were ligated into a TA vector pCR 2.1 (Invitrogen) and electroporated into E.coli DH5a.

text: turning stage fruit

text: fruit

text: Solanum lycopersicum

text: EcoR I

text: EcoR I

text: pericarp

text: tomato thick-juice fruit subtracted by cDNAs of thin-juice fruit

text: plasmid


text: Purdue University

text: live

text: Generic

text: cDNAs made from fruit mRNAs of a thin-juice breeding line (70320) were subtracted by cDNAs made from fruit mRNAs of a thick-juice breeding line (70620) according to the manufacture's protocol of PCR-select cDNA subtraction protocol (Clonetech manual #PT1117-1). The resultant PCR amplified products were ligated into a TA vector pCR 2.1 (Invitrogen) and electroporated into E.coli DH5a.

text: turning stage fruit

text: fruit

text: Solanum lycopersicum

text: EcoR I

text: EcoR I

text: pericarp

text: tomato thin-juice fruit subtracted by cDNAs of thick-juice fruit

text: plasmid


text: Samuel Robets Noble Foundation

text: live

text: Generic

text: Money Maker

text: 4 weeks old seedlings

text: Solanum lycopersicum

text: Leaves

text: Pto overexpressing tomato leaves

text: pBluescript


text: Purdue University

text: live

text: Generic

text: Equal amounts of total RNA isolated from two tomato genotypes differing in juice Bostwick value were mixed and used to prepare a normalized cDNA library (Takahashi-N and Ko-MSH, 1994: Toward a whole cDNA catalog: construction of an equalized cDNA library from mouse embryos. Genomics-San-Diego, 23: 1, 202-210). The resultant PCR amplified products were ligated into pBluscript SKII- vector (Stratagene) and transformed into E. coli DH5alpha

text: Turning stage of fruit ripening.

text: Solanum lycopersicum

text: Pericarp

text: Normalized cDNA library from ripening tomato pericarp


text: Zhejiang University

text: live

text: Generic

text: Moneymaker Cf NIL

text: Tomato seedlings expressing gene pairs Avr9 and Cf-9, or Avr4 and Cf-4, were maintained at 33oC till 7 days after emergency of hypocotyls above soil, then temperature were shifted down to 20oC. Cotyledons were sampled 8 hours after temperature shift for RNA isolation that was directed to cDNA-AFLP analysis.

text: Seedling

text: Cotyleden

text: Solanum lycopersicum

text: Avr4/Cf-4 and Avr9/Cf-9 cDNA-AFLP2003


text: Samuel Robets Noble Foundation

text: live

text: Generic

text: Solanum lycopersicum

text: RG-ptoS

characteristic: strain

text: Lycopersicon esculentum RG-ptoS


text: Samuel Robets Noble Foundation

text: live

text: Generic

text: Solanum lycopersicum

text: Lycopersicon esculentum RG-prf3


text: Samuel Robets Noble Foundation

text: live

text: Generic

text: Solanum lycopersicum

text: RG-ptoR

text: Lycopersicon esculentum RG-ptoR


text: University of Kentucky

text: live

text: Generic

text: MoneyMaker

text: A library comprised of forward and reverse subtracted cDNAs from wild type MoneyMaker and brownseed4 mutant seed from red ripe fruit.

text: Mature

text: Solanum lycopersicum

text: EcoR I

text: Seed

characteristic: tissue-type

text: Mature tomato seed SSH library

text: Plasmid


text: live

text: Generic

text: Transgenic tomato plants that overexpressed Arabidopsis CBF1 grow in Hoagland`s solution normally. The root tissues are used to prepare cDNA library.

text: 1.5-month old plants

text: Solanum lycopersicum

text: NotI

text: SalI

text: root

characteristic: title

text: plasmid


characteristic: External Id

text: live

text: Generic

text: The leaf tissues of normal growth transgenic tomato plants that overexpressed Arabidopsis CBF1 are used to prepare differential display cDNA library.

text: 1.5-month old plants

text: Solanum lycopersicum

text: blunt end ligation

text: blunt end ligation

text: leaf

text: plasmid


text: live

text: Generic

text: The leaf tissues of normal growth transgenic tomato plants that overexpressed Arabidopsis CBF1 are used to prepare subtractive cDNA library.

text: 1.5-month old plants

characteristic: lab-host

text: Solanum lycopersicum

text: blunt end ligation

text: blunt end ligation

text: leaf

text: plasmid


text: live

text: Generic

text: The leaf tissues of normal growth wild-type tomato plants are used to prepare cDNA library.

text: 1.5-month old plants

text: Solanum lycopersicum

text: NotI

text: SalI

text: leaf

text: LEWTL

text: plasmid

characteristic: vector


text: Institute of BioAgricultural Sciences, Academia Sinica

characteristic: INSDC center name

text: live

text: Generic

text: Tomato plants were grown hydroponically in modified Hoagland's solution containing 0.5 mM of KH2PO4. 4-week-old plants were subjected for phosphate starvation treatment by omitting the KH2PO4 from the nutrient solution. Tomato roots were harvested after 7-day phosphate starvation. Total and poly A+ RNA was isolated from phosphate starvation and control roots, respectively. Subtractive cDNA library was constructed using PCR-Select cDNA subtraction Kit (Clontech) to selectively amplify up-regulated genes under phosphate starvation condition.

text: 5 weeks old plants

text: Top 10 F'

text: Solanum lycopersicum

text: root

text: Subtractive cDNA library of roots under phosphate starvation

text: plasmid

text: PGEM Teasy


characteristic: INSDC first public

text: live

text: Generic

text: 1.5-month old plants

text: Solanum lycopersicum

text: blunt end

text: blunt end

text: leaf

text: Tomato leaf of CBF1-5 transgenic plant library

text: plasmid


text: live

text: Generic

text: 1.5-month old plants

characteristic: dev-stage

text: Solanum lycopersicum

text: NotI

text: SalI

text: Root

text: Tomato root of CBF1-5 transgenic plant library

text: plasmid


characteristic: INSDC last update

text: live

text: Generic

text: 1.5-month old plants

text: Solanum lycopersicum

text: blunt end

text: blunt end

text: leaf

text: Tomato leaf of CBF1-6 transgenic plant subtractive library

text: plasmid


text: live

text: Generic

text: 1.5-month old plants

text: Solanum lycopersicum

text: NotI

text: SalI

text: leaf

text: Leaf of wild-type tomato cDNA library

text: plasmid


text: live

text: Generic

text: Subtractive library were created by using mRNA from heat-treated and wildtype tomato roots. Tomato roots were harvested from plants of 3 weeks old seedlings grown in glass jars with MS medium. Plants were heat-treated by incubated at 40oXC in dark for 4 hours followed by recovering at room temperature for 1 hour. Down-regulated library was done by subtracting the non-treated mRNA with the Heat-treated ones.

text: Three weeks old

text: Solanum lycopersicum

text: Roots

text: Subtractive cDNA library of roots (down-regulated) under heat stress

text: plasmid

text: pGEM-T EZ


text: live

text: Generic

text: Subtractive library were created by using mRNA from heat-treated and wildtype tomato roots. Tomato roots were harvested from plants of 3 weeks old seedlings grown in glass jars with MS medium. Plants were heat-treated by incubated at 40oXC in dark for 4 hours followed by recovering at room temperature for 1 hour. Up-regulated library was obtained by subtracting the Heat-treated mRNA with non-treated ones.

text: Three weeks old

text: Solanum lycopersicum

text: Roots

text: Subtractive cDNA library of roots (up-regulated) under heat stress

text: plasmid

text: pGEM-T EZ


text: live

text: Generic

text: Subtractive library were created by using mRNA from heat-treated and wildtype tomato shoots. Tomato shoots were harvested from plants of 3 weeks old seedlings grown in glass jars with MS medium. Plants were heat-treated by incubated at 40oXC in dark for 2 hours followed by recovering at room temperature for 30 minutes.

text: Three weeks old

text: Solanum lycopersicum

text: Shoots

text: Subtractive cDNA library of shoots (up-regulated) under heat stress

text: plasmid

text: pGEM-T EZ


text: live

text: Generic

text: Cadmium treated cDNA V control cDNA(Seedlings treated with 0.2mM CdCl2 for 2 days were harvested. mRNA isolated from each treatment was used for subtracted library construction with PCR-select cDNA subtraction kit (Clontech, Palo Alto, (A)).

text: Two-week-old seedings

text: XL1-Blue, TOP10F'

text: Solanum lycopersicum

text: Seedling

text: Seedlings treated with 0.2mM CdCl2

text: plasmid

text: pGEM-T easy, TOPO


text: live

text: Generic

text: Control cDNA V cadmium treated cDNA (Seedlings treated without 0.2mM CdCl2 for 2 days were harvested. mRNA isolated from each treatment was used for subtracted library construction with PCR-select cDNA subtraction kit (Clontech, Palo Alto, (A)).

text: Two-week-old seedings

text: XL1-Blue, TOP10F'

text: Solanum lycopersicum

text: Seedling

text: Seedlings treated without 0.2mM CdCl2

text: plasmid

text: pGEM-T easy, TOPO


text: Institute of Plant Biology, National Taiwan University

text: live

text: Generic

text: Plant samples were provided by Dr. Jaw-Fen Wang (Tainan, AVRDC). Each plant grown in a 3-inch pot was inoculated with 30 ml (108cfu/ml) of Rs strain Pss4 to give a final concentration of 108cfu/g soil. Roots and collars of inoculated and control plants were harvested 24 and 48 hours post inoculation (e.g. 24-control, 24-inoculated, 48-control and 48-inoculated).Poly(A) RNA was purified from total RNA specimen isolated from individual samples. The poly(A) RNA specimen prepared from 24-control and 48-control samples were mixed at 1:1 ratio. The poly(A) RNA specimen prepared from 24-inoculated and 48-inoculated samples were also mixed at 1:1 ratio. Tomato cDNA library LERSF was then constructed by subtracting the pooled control specimen from the pooled inoculated specimen using CLONTECHPCR-Select cDNA Subtraction kit.

text: Three weeks old

text: Solanum lycopersicum

text: root and collar

text: Subtractive cDNA library prepared from tomato infected with Ralstonia solanacearum (Rs-Ck)

text: plasmid

text: pGEM-T EZ


text: live

text: Generic

text: Plant samples were provided by Dr. Jaw-Fen Wang (Tainan, AVRDC). Each plant grown in a 3-inch pot was inoculated with 30 ml (108cfu/ml) of Rs strain Pss4 to give a final concentration of 108cfu/g soil. Roots and collars of inoculated and control plants were harvested 24 and 48 hours post inoculation (e.g. 24-control, 24-inoculated, 48-control and 48-inoculated).Poly(A) RNA was purified from total RNA specimen isolated from individual samples. The poly(A) RNA specimen prepared from 24-control and 48-control samples were mixed at 1:1 ratio. The poly(A) RNA specimen prepared from 24-inoculated and 48-inoculated samples were also mixed at 1:1 ratio. Tomato cDNA library LERSR was then constructed by subtracting the pooled control specimen from the pooled inoculated specimen using CLONTECHPCR-Select cDNA Subtraction kit.

text: Three weeks old

text: Solanum lycopersicum

text: root and collar

text: Subtractive cDNA library prepared from tomato infected with Ralstonia solanacearum (Ck-Rs)

text: plasmid

text: pGEM-T EZ


text: Institute of BioAgricultural Sciences, Academia Sinica

text: live

text: Generic

text: Tomato plants were grown hydroponically in modified Hoagland's solution. Three-week-old plants were subjected for excess copper treatment in which 10 ugM CuSO4 is applied to the nutrient solution. Roots were harvested after four days of continuous treatment. Total and poly A+ RNA was isolated from treated and control roots, respectively. Subtraction cDNA library was constructed using PCR-Select cDNA subtraction Kit (Clontech) to selectively amplify up-regulated genes under excess copper condition.

text: E. coli DH 5alpha

text: Solanum lycopersicum

text: roots

text: Subtraction cDNA library of root under excess copper

text: plasmid

text: pGEM-T Easy Vector


text: live

text: Generic

text: Tomato CL5915 seeds are obtained from AVRDC.Roots were harvested from plants grown under different developmental stages: 1-,2-,3-,4-month-old. Equal aliquots of mRNA of different developmental stages were mixed and used for cDNA library construction. (Smart PCR cDNA Library construction kit, Clontech)

text: 1-,2-,3-, and 4- month-old

text: E.coli BM25.8

text: Solanum lycopersicum

text: roots

text: Tomato CL5915 roots under different developmental stages

text: plasmid


text: Purdue University

text: live

text: Generic

text: Normalized cDNA library was made from fruit mRNAs of a thin-juice breeding line, Heinz 70320 (tracer) subtracted against cDNA made from fruit mRNA of a thick-juice breeding line, Heinz 70620 (driver) according to the manufacture's protocol of PCR-select cDNA subtraction protocol (Clonetech manual #PT1117-1). The resultant PCR amplified products were ligated into pBluscript SKII- vector (Stratagene) and electroporated into E.coli DH5a.

text: Solanum lycopersicum

text: Pericarp

text: Normalized cDNA library from pericarp of tomato fruit displaying low and high juice viscosity

text: pBluescript SKII-


text: Tel-Aviv University

text: live

text: Generic

text: Hawaii 7981

text: Tomato SSH library derived from reciprocal subtractions of cDNAs prepared from leaf tissues infected with Xanthomonas campestris pv. vesicatoria race T3 or mock-inoculated. cDNAs were subcloned into the pTZ57R plasmid by TA cloning.

text: Full-expanded leaf

text: Solanum lycopersicum

text: Leaf

text: Tomato Xanthomonas-treated suppression subtractive hybridization (SSH) library

text: Plasmid


text: INRA

text: live

text: Generic

text: West Virginia 106

text: 12 days post anthesis

text: Solanum lycopersicum var. cerasiforme

text: fruit

text: Lycopersicon esculentum var. cerasiforme fruit 12 days post anthesis


text: INRA

text: live

text: Generic

text: West Virginia 106

text: 15 days post anthesis

text: Solanum lycopersicum var. cerasiforme

text: fruit

text: Lycopersicon esculentum var. cerasiforme fruit 15 days post anthesis


text: INRA

text: live

text: Generic

text: West Virginia 106

text: 3 days post anthesis

text: Solanum lycopersicum var. cerasiforme

text: fruit

text: Lycopersicon esculentum var. cerasiforme fruit 3 days post anthesis


text: INRA

text: live

text: Generic

text: West Virginia 106

text: 30 days post anthesis

text: Solanum lycopersicum var. cerasiforme

text: fruit

text: Lycopersicon esculentum var. cerasiforme fruit 30 days post anthesis


text: INRA

text: live

text: Generic

text: West Virginia 106

text: 10 days post anthesis

text: Solanum lycopersicum var. cerasiforme

text: fruit

text: Lycopersicon esculentum var. cerasiforme fruit 10 days post anthesis


text: Inra

text: live

text: Generic

text: West Virginia 106

text: 6 DPA

text: Solanum lycopersicum var. cerasiforme

text: fruit

text: Lycopersicon esculentum var. cerasiforme fruit 6 DPA


text: INRA

text: live

text: Generic

text: West Virginia 106

text: 12 dpa

text: Solanum lycopersicum var. cerasiforme

text: fruit

text: Lycopersicon esculentum var. cerasiforme fruit 12 dpa


text: Cornell University

text: live

text: Generic

text: Ailsa Craig

text: A yeast signal sequence trap (YSST) cDNA library was constructed by using poly(A) mRNA isolated from an equal weight mixture of tomato fruits at several different developmental and ripening stages. Poly(A) mRNA was purified from total RNA using an Oligotex mRNA Midi Kit (Qiagen, Valencia, CA) according to the manufacturer's instructions. The first-strand cDNAs were synthesized from mRNA using N6-NotI primer (5'-GAGAGAGAGAGAGAGAACCGCGCGGCCGCCNNNNNN-3') by using a cDNA Synthesis Kit (Stratagene). After second-strand synthesis the cDNAs were prepared for unidirectional cloning by ligation with EcoRI adapters according to the manufacturer's instructions, followed by NotI digestion. The cDNA fragments (0.3 to 1.0 kb) were recovered with a Gel Extraction Kit (Qiagen) from agarose gel, and ligated to an equipartite mixture of the three vectors, pYSST0, pYSST1, and pYSST2, digested with EcoRI and NotI. Ligations were transformed into electrocompetent TOP10 E. coli (Invitrogen) by electroporation.

text: Developmental and ripening stages

text: fruit

characteristic: organ

text: Solanum lycopersicum

text: EcoRI

text: NotI

text: fruit pericarp

text: Tomato fruit related cDNA library-yeast signal sequence trap 1

text: Plasmid

text: pYSST


text: Cornell University

text: live

characteristic: INSDC status

text: Generic

text: Signal sequence trap library of the tomato late blight interactome

text: Solanum lycopersicum

text: SlE


text: Kazusa DNA Research Institute;

text: live

text: Generic

characteristic: NCBI submission model

text: Micro-Tom

text: Solanum lycopersicum

text: maturing fruit

text: Lycopersicon esculentum maturing fruit


text: Kazusa DNA Research Institute;

text: live

text: Generic

characteristic: NCBI submission package

text: Micro-Tom

text: Solanum lycopersicum

text: leaf

text: Lycopersicon esculentum leaf


text: The University of Arizona

text: live

text: Generic

text: Accession PI126449

characteristic: cultivar

text: Glandular trichomes were isolated from leaves of the accession PI126449 using a bead beater, followed by filtration through a series of meshes. DNA sequences were determined bi-directionally using an ABI3730xl at the Arizona Genomics Institute, University of Arizona (H. Kim, D. Kudrna, R.Wing). Chromatogram files were processed to remove vector and cloning oligo sequences and various contaminants, and to trim to a high quality region. Sequences that retained a phred 17 region of at least 100 bases were deposited to GenBank.

text: Mixed stages

text: TJC 121

text: Solanum habrochaites

text: EcoRI

text: EcoRI

text: Glandular Trichome

text: Plasmid

text: pBluescriptSK-


text: Kazusa DNA Research Institute;

text: live

text: Generic

text: Micro-Tom

text: Solanum lycopersicum

characteristic: organism

text: fruit

text: Lycopersicon esculentum cv. Micro-Tom fruit


text: Institute of BioAgricultural Sciences, Academia Sinica

text: live

text: Generic

text: The subtractive library was created by using mRNA purified from tomato roots with or without heat-shock treatment. Tomato roots were harvested from plants of 3 weeks old seedlings grown on MS medium. Plants were heat-treated by incubated at 40 degree C in the dark for 4 h followed by recovery at room temperature for 1 hour. Library of down-regulated cDNA was generated by subtracting the non-treated mRNA with the heat-treated ones.

characteristic: description

text: Three weeks old

text: Solanum lycopersicum

text: roots

text: Tomato root subtractive cDNA library for heat-shock down-regulated genes

text: plasmid

text: pGEM-Teasy


text: live

text: Generic

text: The subtractive library was created by using mRNA purified from tomato roots with or without heat-shock treatment. Tomato roots were harvested from plants of 3 weeks old seedlings grown on MS medium. Plants were heat-treated by incubated at 40 degree C in the dark for 4 h followed by recovery at room temperature for 1 hour. Library of up-regulated cDNA was generated by subtracting the heat-treated mRNA with non-treated ones.

text: Three weeks old

text: Solanum lycopersicum

text: roots

text: Tomato root subtractive cDNA library for heat-shock up-regulated genes

text: plasmid

text: pGEM-Teasy


text: live

text: Generic

text: The subtractive library was created by using mRNA purified from tomato shoots with or without heat-shock treatment. Tomato shoots were harvested from plants of 3 weeks old seedlings grown on MS medium. Plants were heat-treated by incubated at 40 degree C in the dark for 2 h followed by recovery at room temperature for 30 min. Library of up-regulated cDNA was generated by subtracting the heat-treated mRNA with non-treated ones.

text: Three weeks old

text: Solanum lycopersicum

text: shoots

text: Tomato shoot subtractive cDNA library for heat-shock up-regulated genes

text: plasmid

text: pGEM-Teasy


text: Institute of Plant Biology, National Taiwan University

text: live

text: Generic

text: 3 week-old plants (cultivar Hawaii 7996) were inoculated with water (control) or Ralstonia solanacearum (an incompatible strain) (Rs-inoculated). Roots and collars of control and Rs-inoculated plants were harvested 24 and 48 hours post inoculation. Poly(A) RNA specimen prepared from control plant samples were mixed at 1:1 ratio, and that prepared from Rs-inoculated samples were also mixed at 1:1 ratio. Tomato cDNA library was then constructed by subtracting the pooled Rs-inoculated specimen from pooled control specimen using CLONTECH PCR-Select cDNA Subtraction kit.

text: Three weeks old

text: Solanum lycopersicum

text: root and collar

text: Reverse subtractive cDNA library of tomato inoculated with Ralstonia solanacearum

text: plasmid

text: pGEM-Teasy


text: live

text: Generic

text: 3 week-old plants (cultivar Hawaii 7996) were inoculated with water (control) or Ralstonia solanacearum (an incompatible strain) (Rs-inoculated). Roots and collars of control and Rs-inoculated plants were harvested 24 and 48 hours post inoculation. Poly(A) RNA specimen prepared from control plant samples were mixed at 1:1 ratio, and that prepared from Rs-inoculated samples were also mixed at 1:1 ratio. Tomato cDNA library was then constructed by subtracting the pooled control specimen from pooled Rs-inoculated specimen using CLONTECH PCR-Select cDNA Subtraction kit.

text: Three weeks old

text: Solanum lycopersicum

text: root and collar

text: Forward subtractive cDNA library of tomato inoculated with Ralstonia solanacearum

text: plasmid

text: pGEM-Teasy


text: University of Agricultural Sciences

text: live

text: Generic

text: Arka vikas

text: pre-flowering

text: Solanum lycopersicum

text: moisture-stressed leaves

text: DCPSM TH87


text: Wageningen University

text: live

text: Generic

text: MoneyMaker

text: These are single read cDNA-AFLP sequences

text: Solanum lycopersicum

text: whole plants, except for the roots

text: Lycopersicon esculentum cDNA


text: Huazhong Agricultural University

text: live

text: Generic

text: Library constructed using Clontech PCR-Select cDNA Subtraction kit. Tester: root tissue from cultivar LA2711 upon salt treatment; Driver: root tissue from cultivar LA2711 without salt treatment.

text: Solanum lycopersicum

text: 5' EcoRI

text: 3' XhoI

text: root

text: LA2711 Forward SSH library

text: Plasmid

text: pGEM-T easy


text: Huazhong Agricultural University

text: live

text: Generic

text: Library constructed using Clontech PCR-Select cDNA Subtraction kit. Tester: root tissue from cultivar LA2711 without salt treatmen; Driver: root tissue from cultivar LA2711 upon salt treatment.

text: Solanum lycopersicum

text: 5' EcoRI

text: 3' XhoI

text: root

text: LA2711 Reverse SSH library

text: Plasmid

text: pGEM-T easy


text: Huazhong Agricultural University

text: live

text: Generic

text: Library constructed using Clontech PCR-Select cDNA Subtraction kit. Tester: root tissue from cultivar ZS-5 upon salt treatment; Driver: root tissue from cultivar ZS-5 without salt treatment.

text: Solanum lycopersicum

text: 5' EcoRI

text: 3' XhoI

text: root

text: ZS-5 Forward SSH library

text: Plasmid

text: pGEM-T easy


text: Huazhong Agricultural University

text: live

text: Generic

text: Library constructed using Clontech PCR-Select cDNA Subtraction kit. Tester: root tissue from cultivar ZS-5 without salt treatmen; Driver: root tissue from cultivar ZS-5 upon salt treatment.

text: Solanum lycopersicum

text: 5' EcoRI

text: 3' XhoI

text: root

text: ZS-5 Reverse SSH library

text: Plasmid

text: pGEM-T easy


text: University of SFAX, National Institute for Engineering Studies

text: live

text: Generic

text: Baklouti

text: Seeds were germinated in MS medium for 5 days. Seeds RNAs were extracted and used in RT-PCR. RT-PCR fragment was isolated and cloned in pSK+ vector.

text: five days germinated seeds

text: Capsicum annuum

text: HindIII

text: SacI

text: seed

text: Capsicum annuum L. partial CDC2 library

text: Plasmid

text: pBluescriptSK+ vector


text: Laval University

text: live

text: Generic

text: Suppression Subtractive Hybridization cDNA library. Tester: cDNA population from U. americana callus culture infected with O. novo-ulmi subsp. novo ulmi strain H327; (mock) Driver: water. This EST library contains sequences of both the host (U. americana) and the pathogen (O. novo-ulmi subsp. novo-ulmi) with the majority of sequences being of host origin.

text: Escherichia coli (Qiagen EZ competent cells)

text: Ulmus americana

text: PCR product cloning through UA-b

text: PCR product cloning through UA-b

text: Ophiostoma novo-ulmi subsp. novo-ulmi strain H327

characteristic: strain

text: U. americana callus culture

text: Onu-Ua-patha

text: Plasmid

text: pDrive


text: Laval University

text: live

text: Generic

text: Suppression Subtractive Hybridization cDNA library. Tester: cDNA population from U. americana callus culture infected with O. novo-ulmi subsp. novo ulmi strain H327; Driver: cDNA population from healthy U. americana callus culture. This EST library contains sequences of both the host (U. americana) and the pathogen (O. novo-ulmi subsp. novo-ulmi) with the majority of sequences being of host origin.

text: Ulmus americana

text: PCR product cloning through UA-b

text: PCR product cloning through UA-b

text: U. americana callus culture

characteristic: tissue-type

text: Onu-Ua-pathb

text: Plasmid

text: pDrive


text: Laval University

text: live

text: Generic

text: Suppression Subtractive Hybridization cDNA library. Tester: cDNA population from U. americana callus culture infected with O. novo-ulmi subsp. novo ulmi strain H327; Driver: mix of cDNA populations from healthy U. americana callus culture and axenically grown O. novo-ulmi subsp. novo-ulmi H327 mycelium. This EST library contains sequences of both the host (U. americana) and the pathogen (O. novo-ulmi subsp. novo-ulmi) with the majority of sequences being of host origin.

text: Ulmus americana

text: PCR product cloning through UA-b

text: PCR product cloning through UA-b

text: U. americana callus culture

text: Onu-Ua-pathc

characteristic: title

text: Plasmid

text: pDrive


characteristic: External Id

text: National Agronomic Institute of Tunisia

text: live

text: Generic

text: seedlings

text: Cicer arietinum

text: hermaphrodite

characteristic: sex

text: leaves

text: Chickpea pGEMT cDNA clones

text: Plasmid

text: pGEMT easy vector


text: Kunming Insitute of Botany, Chinese Academy of Sciences

text: live

text: Generic

text: Saururus chinensis

text: Specific subtracted floral cDNA library of Saururus chinensis


text: National Bureau of Animal Genetics Resources

text: live

text: Generic

text: non-lactating stage

text: E.coli DH5 alpha

characteristic: lab-host

text: Bubalus bubalis

text: SfiI

text: SfiI

text: Female

text: Mammary gland

text: cDNA library from non-lactating buffalo mammary gland

text: Plasmid

text: pDNR -LIB

characteristic: vector


text: University of Liverpool

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: cDNA library prepared from roach alverton spawned Males (adult)

text: Adult

text: Gonads

text: Rutilus rutilus

text: Sfi1 GGCCATTACGGCC

text: Sfi1 GGCCGCCTCGGCC

text: Male

text: CSM

text: Plasmid


text: University of Liverpool

characteristic: INSDC last update

text: live

text: Generic

text: cDNA library prepared from roach calverton spawned Females (adult)

text: Adult

characteristic: dev-stage

text: Gonads

text: Rutilus rutilus

text: Sfi1 GGCCATTACGGCC

text: Sfi1 GGCCGCCTCGGCC

text: Female

text: CSF

text: Plasmid


text: University of Liverpool

text: live

characteristic: INSDC status

text: Generic

text: Normalized cDNA library prepared from roach 1 year males

text: Gonads

characteristic: organ

text: Rutilus rutilus

text: Sfi1 GGCCATTACGGCC

text: Sfi1 GGCCGCCTCGGCC

text: Male

text: Plasmid


text: University of Liverpool

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Normalized cDNA library prepared from roach 1 year females

text: Gonads

text: Rutilus rutilus

text: Sfi1 GGCCATTACGGCC

text: Sfi1 GGCCGCCTCGGCC

text: Female

text: Plasmid


text: University of Liverpool

text: live

text: Generic

text: cDNA library prepared from roach 250dph (days post hatch) control (not exposed to EDCs)

text: Gonads

text: Rutilus rutilus

characteristic: organism

text: Sfi1 GGCCATTACGGCC

text: Sfi1 GGCCGCCTCGGCC

text: Male

text: 250DPH (.)

text: Plasmid


text: University of Liverpool

text: live

text: Generic

text: cDNA library prepared from roach 250dph (days post hatch) exposed (exposed to EDCs)

characteristic: description

text: Gonads

text: Rutilus rutilus

text: Sfi1 GGCCATTACGGCC

text: Sfi1 GGCCGCCTCGGCC

text: Male

text: 250DPH (..)

text: Plasmid


text: University of California

text: live

text: Generic

characteristic: cultivar

text: PCR amplified cDNA using C2S4 and VIGS-F primers and direct cloning in pCRII-TOPO TA cloning vector.

text: mature

text: E. coli

text: Solanum arcanum

text: leaf

text: Solanum arcanum Mi homologue TOPO library

text: plasmid

text: pCRII-TOPO


text: Korea Research Institute of Bioscience & Biotechnology

text: live

text: Generic

text: Pacifica Deep Orchid

text: Total RNAs were prepared from young leaves of mature plant grown in greenhouse using a hot-phenol procedure and then polyA RNAs were purified using the PolyATract mRNA isolation system (Promega, Madison, Wis.). The cDNA synthesis and library construction was performed according to the manufacturer's instructions (Stratagene, San Diego, Calif.). We used 1x106 pfu of the primary library to construct a phagemid library.

text: mature plants grown in greenhouse

text: Catharanthus roseus

text: Leaf

characteristic: tissue-type

text: Leaf CR02 cDNA library

text: Uni-ZAP XR


text: live

text: Generic

text: Pacifica Deep Orchid

text: Total RNAs were prepared from roots of seedlings using a hot-phenol procedure and then polyA RNAs were purified using the PolyATract mRNA isolation system (Promega, Madison, Wis.). The cDNA synthesis and library construction was performed according to the manufacturer's instructions (Stratagene, San Diego, Calif.). We used 1x106 pfu of the primary library to construct a phagemid library.

text: 3 weeks old seedling grown on MS medium

text: Catharanthus roseus

text: Root

text: Root CR03 cDNA library

characteristic: title

text: Uni-ZAP XR


characteristic: External Id

text: live

text: Generic

text: Total RNA was isolated from two C. roseus cell suspension cultures elicited at 1 and 4 h with 0.05% yeast extract (Difco). The total RNA extracted from both cultures was mixed in a 1:1 ratio. Poly(A)+ RNA was isolated using a Promega kit. The cDNA was synthesized and inserted in lambda-ZAP using the ZAP-cDNATM kit from Stratagene. (J Biol Chem, Vol. 275, Issue 5, 3051-3056, February 4, 2000)

text: Catharanthus roseus

text: cell suspension culture

text: CR04 cDNA library

text: lamda-ZAP


text: live

text: Generic

text: Total RNA was isolated from C. roseus cell suspension line cultured in induction medium. The cDNA was synthesized and inserted in lambda-ZAP using the ZAP-cDNATM kit from Stratagene.

text: Catharanthus roseus

text: cell suspension culture

text: CR05 cDNA library

text: lambda-ZAP


text: live

text: Generic

text: A subtracted cDNA library was constructed by subtractive suppression hybridization (SSH) using the PCR-Select cDNA subtraction kit (BD Biosciences, USA). Hairy root treated with methyl jasmonate was used as testers, while non-treated hairy root was used as drivers.

text: Catharanthus roseus

text: Hairy root

text: Catharanthus roseus Subtraction library

text: pCR4 Blunt-TOPO


text: live

text: Generic

text: Cooler rose

text: Total RNAs were prepared from flower buds of mature plant grown in greenhouse using a hot-phenol procedure and then polyA RNAs were purified using the PolyATract mRNA isolation system (Promega, Madison, Wis.). The cDNA synthesis and library construction was performed according to the manufacturer's instructions (Stratagene, San Diego, Calif.). We used 1x106 pfu of the primary library to construct a phagemid library.

text: mature plants grown in greenhouse

text: Catharanthus roseus

text: Flower bud

text: Flower bud CR01 cDNA library

text: Uni-ZAP XR


text: Texas Tech University Health Sciences Center

text: live

text: Generic

text: peritubular cells

characteristic: cell type

text: Primary, unamplified cDNA libraries from 18-20 day mouse peritubular cells were constructed in lambda Uni-Zap XR (Stratagene, CA) as directionally cloned from oligo(dT)-primed cDNA from Swiss (CD-1) mouse testis poly(A)+ RNA (See McCarrey, et al. J. Androl. 1999 20:635-639). Plasmid rescues were performed by mass excision on these libraries according to the manufacturer's protocol (Stratagene, CA). cDNA oligo dT-primed [5'-(GA)10-ACTAGTCTCGAGTTTTTTTTTTTTT-3'] and directionally cloned using 5' linkers 5'-AATTCGGCACGAG-3' and 5'-CTCGTGCCG-3'. Size selection of > 400bp material gives average insert size ranging from 1-2 kb. Original lambda-based library is available through ATCC, catalog #63428.

text: 18-20 day

text: Escherichia coli (XL2 Blue MRF')

text: testis

text: Mus musculus

text: XhoI

text: EcoRI

text: male

characteristic: sex

characteristic: strain

text: peritubular cells

text: MacDonald peritubular cells

text: Plasmid

text: Stratagene lambda Uni-Zap XR


characteristic: INSDC center name

text: live

text: Generic

text: Sertoli cells

text: Primary, unamplified cDNA libraries from 18-20 day mouse Sertoli cells (10 pfu/ul) were constructed in lambda Uni-Zap XR (Stratagene, CA) as directionally cloned from oligo(dT)-primed cDNA from Swiss (CD-1) mouse testis poly(A)+ RNA (See McCarrey, et al. J. Androl. 1999 20:635-639). Plasmid rescues were performed by mass excision on these libraries according to the manufacturer's protocol (Stratagene, CA). cDNA oligo dT-primed [5'-(GA)10-ACTAGTCTCGAGTTTTTTTTTTTTT-3'] and directionally cloned using 5' linkers 5'-AATTCGGCACGAG-3' and 5'-CTCGTGCCG-3'. Size selection of > 400bp material gives average insert size ranging from 1-2 kb. Original lambda-based library is available through ATCC, catalog #63427.

text: 18-20 day

text: Escherichia coli (XL2 Blue MRF')

characteristic: lab-host

text: testis

text: Mus musculus

text: XhoI

text: EcoRI

text: male

text: Sertoli cells

text: MacDonald Sertoli cells

text: Plasmid

text: Stratagene lambda Uni-Zap XR

characteristic: vector


characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: round spermatids

text: Primary, unamplified cDNA libraries from adult mouse round spermatids (5.5 pfu/ul) were constructed in lambda Uni-Zap XR (Stratagene, CA) as directionally cloned from oligo(dT)-primed cDNA from Swiss (CD-1) mouse testis poly(A)+ RNA (See McCarrey, et al. J. Androl. 1999 20:635-639). Plasmid rescues were performed by mass excision on these libraries according to the manufacturer's protocol (Stratagene, CA). cDNA oligo dT-primed [5'-(GA)10-ACTAGTCTCGAGTTTTTTTTTTTTT-3'] and directionally cloned using 5' linkers 5'-AATTCGGCACGAG-3' and 5'-CTCGTGCCG-3'. Size selection of > 400bp material gives average insert size ranging from 1-2 kb. Original lambda-based library is available through ATCC, catalog #63423.

text: adult

characteristic: dev-stage

text: Escherichia coli (XL2 Blue MRF')

text: testis

text: Mus musculus

text: XhoI

text: EcoRI

text: male

text: round spermatids

text: MacDonald round spermatids

text: Plasmid

text: Stratagene lambda Uni-Zap XR


text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: primitive type A spermatogonia

text: Primary, unamplified cDNA libraries from mouse primitive Type A spermatogonia (0.116 pfu/ul) were constructed in lambda Uni-Zap XR (Stratagene, CA) as directionally cloned from oligo(dT)-primed cDNA from Swiss (CD-1) mouse testis poly(A)+ RNA (See McCarrey, et al. J. Androl. 1999 20:635-639). Plasmid rescues were performed by mass excision on these libraries according to the manufacturer's protocol (Stratagene, CA). cDNA oligo dT-primed [5'-(GA)10-ACTAGTCTCGAGTTTTTTTTTTTTT-3'] and directionally cloned using 5' linkers 5'-AATTCGGCACGAG-3' and 5'-CTCGTGCCG-3'. Size selection of > 400bp material gives average insert size ranging from 1-2 kb. Original lambda-based library is available through ATCC, catalog #63415.

text: 6 day

text: Escherichia coli (XL2 Blue MRF')

text: testis

characteristic: organ

text: Mus musculus

text: XhoI

text: EcoRI

text: male

text: primitive type A spermatogonia

text: MacDonald primitive type A spermatogonia

text: Plasmid

text: Stratagene lambda Uni-Zap XR


text: live

text: Generic

characteristic: NCBI submission package

text: pachytene spermatocytes

text: Primary, unamplified cDNA libraries from adult mouse pachytene spermatocytes (3.7 pfu/ul) were constructed in lambda Uni-Zap XR (Stratagene, CA) as directionally cloned from oligo(dT)-primed cDNA from Swiss (CD-1) mouse testis poly(A)+ RNA (See McCarrey, et al. J. Androl. 1999 20:635-639). Plasmid rescues were performed by mass excision on these libraries according to the manufacturer's protocol (Stratagene, CA). cDNA oligo dT-primed [5'-(GA)10-ACTAGTCTCGAGTTTTTTTTTTTTT-3'] and directionally cloned using 5' linkers 5'-AATTCGGCACGAG-3' and 5'-CTCGTGCCG-3'. Size selection of > 400bp material gives average insert size ranging from 1-2 kb. Original lambda-based library is available through ATCC, catalog #63422.

characteristic: description

text: adult

text: Escherichia coli (XL2 Blue MRF')

text: testis

text: Mus musculus

characteristic: organism

text: XhoI

text: EcoRI

text: male

text: pachytene spermatocytes

text: MacDonald pachytene spermatocytes

text: Plasmid

text: Stratagene lambda Uni-Zap XR


text: Univ. of Tokai School of Med.

text: live

text: Generic

text: composite sequence

text: brain

text: Homo sapiens

text: brain

characteristic: tissue-type

text: Human brain cDNA composite sequence

characteristic: title

text: lambda zapII


characteristic: External Id

text: Univ. of Tokai School of Med.

text: live

text: Generic

text: striata

text: brain

text: Homo sapiens

text: brain

text: Human brain cDNA striata

text: lambda zapII


text: Univ. of Tokai School of Med.

text: live

text: Generic

text: temporal cortex

text: brain

text: Homo sapiens

text: brain

text: Human brain cDNA temporal cortex

text: lambda zapII


text: Departement de Biochimie, Universite de Montreal

text: live

text: Generic

text: Euglena gracilis - Gray & Durnford group

text: Euglena gracilis

text: Euglena gracilis Non-normalized


text: live

text: Generic

text: Euglena gracilis

text: Euglena gracilis Normalized long fraction


text: live

text: Generic

text: Euglena gracilis

text: Euglena gracilis Normalized small


text: Faculty of Veterinary medicine, Universite de Montreal

text: live

text: Generic

text: granulosa and oocyte

characteristic: cell type

text: PCR fragment; cDNA library (OF-DF) made from granulosa cells and oocytes of ovulatory follicles (OF) obtained 24 hours after human chorionic gonadotropin injection that was subtracted from granulosa and oocytes of dominant follicles (DF) collected at day 5 of the estrous cycle.

text: ovulatory follicle

text: ovary

text: Bos taurus

text: female

characteristic: sex

text: Holstein

text: Bos taurus subtracted cDNA of granulosa cells and oocytes from human chorionic gonadotropin-treated ovulatory follicles (OF-DF)


text: College of Animal Science and Technology, Northwest A & F University

characteristic: INSDC center name

text: live

text: Generic

text: Capra hircus

text: Female

text: Xinong Saanen goat

characteristic: strain

text: Mammary gland

text: Xinong Saanen goat mammary gland


text: Glasgow Caledonian University

text: live

text: Generic

text: Directionally cloned normalised full-length cDNA library constructed using poly A+ RNA from the indicated tissue of chemically-exposed (Cu, DBA, EE2) male stickleback.

text: mature

text: liver

text: Gasterosteus aculeatus

text: male

text: hepatic

text: phage

text: pTriplEx2 (Clontech)

characteristic: vector


text: Glasgow Caledonian University

characteristic: INSDC first public

text: live

text: Generic

text: Directionally cloned normalised full-length cDNA library constructed using poly A+ RNA from the indicated tissue of chemical-treated (Cu, DBA, EE2) male stickleback.

text: mature

text: liver

text: Gasterosteus aculeatus

text: male

text: hepatic

text: plasmid

text: pDNR Lib (Clontech)


text: Glasgow Caledonian University

characteristic: INSDC last update

text: live

text: Generic

text: Subtracted cDNA library constructed using poly A+ RNA from the indicated tissue of control and chemically-treated (Cu, DBA, EE2) male stickleback.

text: mature

text: liver

text: Gasterosteus aculeatus

text: male

text: hepatic

text: plasmid

text: pT7 Blue3 (Novagen)


text: Center of Marine Sciences, FCMA, University of Algarve

text: live

text: Generic

text: Cells grown for 36 h in either 9% NaCl low nitrate medium or in 18% NaCl low nitrate medium were used as reference ('driver') and 'tester', respectively.

text: Dunaliella salina

text: CCAP 19/30

text: Dunaliella salina subtracted cDNA library

text: Plasmid

text: pGEM-T Easy


text: Plant Research International B.V.

text: live

text: Generic

text: The subtractive library was created by using pools of mRNA isolated from salt treated tomato roots of salt sensitive tomato plants. The salt sensitive tomato plants were obtained from an F6 recombinant inbred population of the cross between L. esculentum and L. pimpinellifolium. Roots were harvested from 8 week old plants grown in a moderate (E.C. 8.0) and low (E.C. 2.5) saline environment in a hydroponic system.

text: Eight week old

characteristic: dev-stage

text: Solanum lycopersicum x Solanum pimpinellifolium

text: root

text: Subtractive cDNA library (down-regulated) of salt sensitive tomato grown in a moderate and low saline environment

text: Plasmid

text: pGEM-T easy


text: Plant Research International B.V.

text: live

characteristic: INSDC status

text: Generic

text: The subtractive library was created by using pools of mRNA isolated from salt treated tomato roots of salt sensitive and salt tolerant plants. The salt sensitive and salt tolerant plants were obtained from an F6 recombinant inbred population of the cross between L. esculentum and L. pimpinellifolium. Roots were harvested from 8 week old plants grown under moderate saline conditions (E.C. 8) in a hydroponic system.

text: Eight week old

text: root

text: Subtractive cDNA library (Up-regulated) of salt tolerant versus sensitive tomato plants grown in a saline environment

text: Plasmid

text: pGEM-T easy


text: Plant Research International B.V.

text: live

text: Generic

characteristic: NCBI submission model

text: Eight week old

text: root

text: Subtractive cDNA library (Down-regulated) of salt tolerant versus sensitive tomato plants grown in a saline environment

text: Plasmid

text: pGEM-T easy


text: Plant Research International B.V.

text: live

text: Generic

text: The subtractive library was created by using pools of mRNA isolated from salt treated tomato roots of salt tolerant plants. The salt tolerant plants were obtained from an F6 recombinant inbred population of the cross between L. esculentum and L. pimpinellifolium. Roots were harvested from 8 week old plants grown under moderate (E.C. 8) and low (E.C. 2.5) saline conditions in a hydroponic system.

text: Eight week old

text: root

text: Subtractive cDNA library (up-regulated) of salt tolerant tomato plants grown in a moderate and low saline environment

text: Plasmid

text: pGEM-T easy


text: Plant Research International B.V.

text: live

text: Generic

text: Eight week old

text: root

text: Subtractive cDNA library (down-regulated) of salt tolerant tomato plants grown in a moderate and low saline environment

text: Plasmid

text: pGEM-T easy


text: Plant Research International B.V.

text: live

text: Generic

text: The subtractive library was created by using pools of mRNA isolated from salt treated tomato roots of salt sensitive tomato plants. The tomato plants were obtained from an F6 recombinant inbred population of the cross between L. esculentum and L. pimpinellifolium. Roots were harvested from 8 week old plants grown in a moderate (E.C. 8.0) and low (E.C. 2.5) saline environment in a hydroponic system.

text: Eight week old

text: root

text: Subtractive cDNA library (up-regulated) of salt sensitive tomato grown in a moderate and low saline environment

text: Plasmid

text: pGEM-T easy


text: The Institute for Genomic Research (TIGR; www.tigr.org)

text: live

text: Generic

text: Hale

characteristic: cultivar

text: Leaves

characteristic: organ

text: Ricinus communis

text: XhoI

text: EcoRI

text: RBAA-Castor bean cDNA library from leaves,680bp

text: pbsII SK+


text: live

text: Generic

characteristic: NCBI submission package

text: Hale

text: Leaves

text: Ricinus communis

text: XhoI

text: EcoRI

text: Castor bean cDNA library from flowers,800bp

text: pbsII SK+


text: Indian Institute of Technology, Kharagpur

text: live

text: Generic

text: One day old larvae

text: Antheraea mylitta

characteristic: organism

text: one day old larvae library

text: phagemid

text: pBluescript SK-


text: live

text: Generic

text: fifth instar larvae

text: silkgland

text: Antheraea mylitta

text: silkglands of fifth instar larvae library

text: phagemid

text: pBluescript SK-


text: University of California

text: live

text: Generic

text: Seeds were germinated in pots (Ehlers) and grown in controlled glass house conditions under natural photoperiod and day/night temperatures of 35/18oC. The growing axillary buds from 10 day old seedlings were harvested from one set of pots, flash frozen and stored at -80oC. Another set of plants was allowed to grow under drought stress induced by withholding water. The growing axillary buds were harvested from 4-day drought stressed seedlings, then flash frozen (Sudhakar). The frozen samples were used for RNA extraction by a TRIzol method (Sudhakar, Mandal, Fenton). A total of 500 microgram each from two pools of three stressed and three unstressed sub-samples were combined to total 1000 microgram of total RNA. mRNA was isolated using PolyATtrack (Promega). Five micrograms of poly(A) mRNA was used for cDNA synthesis (Stratagene). A primary cDNA library was produced using a lambda ZAP XR cDNA Synthesis Kit(Stratagene). One million pfu from the primary library were mass excised to produce a phagemid population (Sudhakar). Phagemids were plated, plasmid DNA purified and sequences studied by students (Finaldi, Pottorff, Pena, Hasegawa, Hasegawa, McCoy, Ramirez, Wong, Alzubi, Ngo) and the teaching assistant (Schroeder) in the Fall 2006 'Plant Genomics and Biotechnology' class taught by Close at UC Riverside. Plasmids were readied for sequencing (Close, Resnik) and DNA sequences determined bi-directionally using an ABI3730 at the Genomics Core Instrumentation facility, University of California, Riverside (Choi). Sequences were processed (Wanamaker) using the HarvEST pipeline (http://harvest.ucr.edu) to remove vector and cloning oligo sequences and various contaminants, and to trim to a high quality region. Sequences that retained a phred 17 region of at least 100 bases were deposited to GenBank.

characteristic: description

text: Seedling

text: E. coli TJC121

characteristic: lab-host

text: Vigna unguiculata

text: EcoRI

text: XhoI

text: growing axillary bud

characteristic: tissue-type

text: Cowpea CB27 Axillary Bud cDNA Library - UCRVU02-2

text: Phagemid pBluescript SK(-)

text: Lambda Uni-ZAP XR, excised phagemid


characteristic: External Id

text: Purdue University, Department of Horticulture and Landscape Architecture

text: live

text: Generic

text: Phaseolus vulgaris

text: Common bean supression subtractive hybridization library

characteristic: title


text: The Horticulture and Food Research Institute of New Zealand Ltd

characteristic: INSDC center name

text: live

text: Generic

text: Forward Primer Name: LM638 (ggnggngtnggnaanacnac) Reverse Primer Name: LM637 (arngctarnggnarncc)

text: Malus domestica

text: Leaf

text: (ABHA) Pinkie resistance gene analogue PCR subclone library

text: pBluescript SK(-)


text: Johannes Gutenberg University of Mainz

text: live

text: Generic

text: expressed sequence tag, 5'read; Oligo dT-primed; unidirectionally cloned via Sal I (5') and Not I (3'); 5' adapter sequence: TCGACCCACGCGTCCG; 3' primer sequence: GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT; sequencing primer: T7; average insert size 1.4 kb.

text: Xenoturbella bocki

text: Sal I

text: Not I

text: Xenoturbella bocki library


text: New Zealand Institute for Crop & Food Research

text: live

text: Generic

text: country: New Zealand:Lincoln

text: 30 days post-infection

text: Arabidopsis thaliana Col-0

text: Plasmodiophora brassicae

text: galls (on root and hypocotyl)


text: NRC Institute for Marine Biosciences Industrial Partnership Facility

text: live

text: Generic

text: Gadus morhua

text: heart

text: Gadus morhua heart library gmapht


text: live

text: Generic

text: Gadus morhua

text: ovary

text: Gadus morhua ovary library gmapov


text: Norwegian School of Veterinary Science

text: live

text: Generic

text: Adult

text: Salmo salar

text: EcoRI

text: NotI

text: Brain

text: Atlantic salmon normalized Brain cDNA library

text: Plasmid


text: Norwegian School of Veterinary Science

text: live

text: Generic

text: Adult

text: Salmo salar

text: EcoRI

text: NotI

text: Muscle

text: Atlantic salmon normalized Muscle cDNA library

text: Plasmid


text: University of Messina

text: live

text: Generic

text: Gills

text: Coris julis

text: Coris julis gills cDNA RT-PCR


text: GenApps Inc.

text: live

text: Generic

text: Burley, 4407

text: Nicotiana tabacum

text: Leaf

text: Ethylene Induced Tobacco Leaf cDNA Library


text: The Institute for Genomic Research (TIGR; www.tigr.org)

text: live

text: Generic

text: Hale

text: The cDNA was generated using an oligo dT primer with a 3' NotI site. The 5' end of the cDNA was blunt-end ligated to the EcoRV site and the 3' end to the NotI site of the pCMV-SPORT6.1 vector. The average insert size of the library is 1.7 kb.

text: seed

text: Ricinus communis

text: Castor bean cDNA library from leaves, 1.7 kb


text: live

text: Generic

text: Hale

text: The cDNA was generated using an oligo dT primer with a 3' NotI site. The 5' end of the cDNA was blunt-end ligated to the EcoRV site and the 3' end to the NotI site of the pCMV-SPORT6.1 vector. The average insert size of the library is 1.8 kb.

text: Root

text: Ricinus communis

text: Castor bean cDNA library from roots, 1.8 kb


text: live

text: Generic

text: Hale

text: The cDNA was generated using an oligo dT primer with a 3' NotI site. The 5' end of the cDNA was blunt-end ligated to the EcoRV site and the 3' end to the NotI site of the pCMV-SPORT6.1 vector. The average insert size of the library is 1.4 kb.

text: 12-33 days after pollination

text: seed

text: Ricinus communis

text: Castor bean cDNA library from seeds (12-33 days after pollination), 1.4 kb


text: live

text: Generic

text: Hale

text: The cDNA was generated using an oligo dT primer with a 3' NotI site. The 5' end of the cDNA was blunt-end ligated to the EcoRV site and the 3' end to the NotI site of the pCMV-SPORT6.1 vector. The average insert size of the library is 1.6 kb.

text: 40-61 days after pollination

text: seed

text: Ricinus communis

text: Castor bean cDNA library from seeds (40-61 days after pollination), 1.6 kb


text: Department of Plant Biotechnology, College of Life Resource Sciences, Dongguk University

text: live

text: Generic

text: Jinpumkong, Cheongjakong

text: Plants from which samples were collected at V5 stage after planting were used. V5 stage plants were exposed to a single dose of 200 ppb ozone for each 4 h per day during 5 days.

text: Glycine max

text: leaves

text: Substract library of ozone treated leaves in Glycine max


text: National Eye Institute

text: live

text: Generic

text: RNA was extracted from pooled rhesus monkey lens. A directionally cloned cDNA library in the pCMVSPORT6 vector (Invitrogen) was constructed at Bioserve Biotechnology (Laurel MD) essentially following the protocols of the SuperScript Plasmid System, full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. cDNA was cloned in Not I/Sal I sites. EST analysis was performed at the NIH Intramural Sequencing Center (NISC). Analyzed data available through http://neibank.nei.nih.gov.

text: adult

text: Eye

text: Macaca mulatta

text: Lens

text: Rhesus monkey lens. Unnormalized (nbl)

text: Plasmid


text: National Eye Institute

text: live

text: Generic

text: RNA was extracted from pooled cow lens. A directionally cloned cDNA library in the pCMVSPORT6 vector (Invitrogen) was constructed at Bioserve Biotechnology (Laurel MD) essentially following the protocols of the SuperScript Plasmid System, full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. cDNA was cloned in Not I/Sal I sites. EST analysis was performed at the NIH Intramural Sequencing Center (NISC). Analyzed data available through http://neibank.nei.nih.gov.

text: adult

text: Eye

text: Bos taurus

text: Lens

text: Cow lens. Unnormalized (nbm)

text: Plasmid


text: Departement de Biochimie, Universite de Montreal

text: live

text: Generic

text: Guillardia theta

text: Guillardia theta non-normalised cDNA library


text: live

text: Generic

text: Guillardia theta

text: Guillardia theta Non-normalized


text: live

text: Generic

text: Guillardia theta

text: Guillardia theta non-normalised


text: live

text: Generic

text: Oxyrrhis marina

text: Oxyrrhis marina Non-normalized


text: live

text: Generic

text: Zootermopsis symbionts

text: Eukaryota sp. Zootermopsis hindgut protist

text: hindgut material of the pacific damp-wood termite Zootermopsis


text: University of Victoria

text: live

text: Generic

text: Tissue contributors: Robert Devlin (DFO, Vancouver, B.C.)

text: thymus

text: Salmo salar

text: McConnell

characteristic: strain

text: ssalevd thymus Salmo salar cDNA


text: University of Victoria

text: live

text: Generic

text: thyroid

text: Salmo salar

text: McConnell

text: ssaleve thyroid Salmo salar cDNA

characteristic: vector


text: Instituto Superior De Agronomia

characteristic: INSDC first public

text: live

text: Generic

text: Torero

text: stressed plant

text: Triticum aestivum

text: Triticum aestivum cv. Torero stressed plant


text: Sabanci University

characteristic: INSDC last update

text: live

text: Generic

text: Leaf tissues were collected 24 hours after infection

text: Festuca rubra subsp. rubra

text: Leaf

text: Creeping red fescue leaf tissue


text: Children's Memorial Research Center

text: live

text: Generic

text: CMRC-CP-II0 library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CCTGAATAG. Tissue was obtained from the Provasoli-Guillard National Center for Culture of Marine Phytoplankton (CCMP).

text: DH10B T1 phage resistant (Invitrogen)

text: Cyanophora paradoxa

text: EcoR I

text: Not I

text: pT7T3-Pac (Pharmacia) with a modified polylinker


text: Institute of Plant Molecular Biology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University

text: live

text: Generic

text: Onosma paniculata

text: cDNA library of Onosma paniculatum cell cultures


text: Kazusa DNA Research Institute;

text: live

text: Generic

text: Micro-Tom

text: synonym: Lycopersicon esculentum

text: Solanum lycopersicum

text: leaf

text: Solanum lycopersicum cv. Micro-Tom leaf


text: Kazusa DNA Research Institute;

text: live

text: Generic

text: Micro-Tom

text: synonym: Lycopersicon esculentum

text: Solanum lycopersicum

text: fruit

text: Solanum lycopersicum cv. Micro-Tom fruit


text: National Institute of Genetics

text: live

text: Generic

text: gastrula

characteristic: dev-stage

text: Halocynthia roretzi

text: hermaphrodite

characteristic: sex

text: whole animal

text: Kazuhiro W. Makabe unpublished cDNA library, gastrula


text: National Institute of Genetics

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: cleaving embryo

text: Halocynthia roretzi

text: hermaphrodite

text: whole animal

text: Kazuhiro W. Makabe unpublished cDNA library, cleaving embryo


text: National Institute of Genetics

text: live

text: Generic

text: neurula

text: Halocynthia roretzi

text: hermaphrodite

text: whole animal

text: Kazuhiro W. Makabe unpublished cDNA library, neurula


text: National Botanical Research Institute

text: live

text: Generic

text: Subtractive hybridization technique apply to compare Berberis chitria and Berberis asiatica at mRNA-cDNA level.

text: Berberis chitria

text: Berberis chitria clones partial cDNA seauence


text: UC Los Angeles

text: live

text: Generic

text: Adult (whole animal)

text: Macrostomum lignano

text: Hermaphrodite

text: Macrostomum lignano Adult LT Library

text: Plasmid

text: pCMV Sport6.1


text: Northeast Forestry University

text: live

text: Generic

text: Tamarix hispida roots were treated with NaCl for 0,24,48 hours before cDNA library construction.

text: Tamarix hispida

text: sfiI

text: sfiI

text: Root

text: Tamarix hispida roots

text: Plasmid

text: pDNR-LIB


text: United States Department of Agriculture

text: live

text: Generic

text: Gale Gala

characteristic: cultivar

text: Malus domestica

text: young leaf

text: Erwinia amylovora challenged cDNA suppression subtractive hybridization library early upregulated genes


text: Sabanci University

text: live

text: Generic

characteristic: NCBI submission package

text: Cynodon dactylon

text: Roots

text: Bermudagrass root tissue


text: Sabanci University

text: live

text: Generic

text: Cynodon dactylon

characteristic: organism

text: Shoots

text: Bermudagrass shoot tissue


text: Sabanci University

text: live

text: Generic

text: Apache

text: Lolium arundinaceum

text: Roots

text: Tall fescue root tissue


text: Sabanci University

text: live

text: Generic

text: Apache

text: Lolium arundinaceum

text: Shoots

text: Tall fescue shoot tissue


text: Universite Claude Bernard Lyon 1

text: live

text: Generic

text: country: France:South West France, Cap Ferret; environmental_sample; isolation_source: sandy soil from a Pinus pinaster forest

characteristic: description

text: uncultured eukaryote

text: uncultured eukaryote from sandy soil from a Pinus pinaster forest