text: Concordia University

text: live

characteristic: INSDC status

text: Generic

text: Complementary DNA was synthesized with ZAP kit (Stratagene) using poly(A)+RNA isolated from Aureobasidium pullulans cultured under different carbon sources. Synthesis was primed with oligo(dT)/XhoI primer. EcoRI adaptors were ligated to the bunt-ended, double-stranded cDNA. The EcoRI-XhoI-digested cDNA was ligated with EcoRI-XhoI-digested pBluescript KS+ (Invitrogen Corp).

text: E. coli

text: Aureobasidium pullulans

text: XhoI

text: EcoRI

text: Aureobasidium pullulans pBluescript (EcoRI-XhoI)

text: plasmid

text: pBluescript KS+


status: PUBLIC

characteristic: External Id

text: Concordia University

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Complementary DNA was synthesized with ZAP kit (Stratagene) using poly(A)+RNA isolated from Amorphotheca resinae cultured under different carbon sources. Synthesis was primed with oligo(dT)/XhoI primer. EcoRI adaptors were ligated to the bunt-ended, double-stranded cDNA. The EcoRI-XhoI-digested cDNA was ligated with EcoRI-XhoI-digested pBluescript KS+ (Invitrogen Corp).

characteristic: description

text: E. coli

tag: attribute

characteristic: lab-host

text: Amorphotheca resinae

characteristic: organism

text: XhoI

text: EcoRI

text: Amorphotheca resinae pBluescript (EcoRI-XhoI)

characteristic: title

text: plasmid

text: pBluescript KS+

characteristic: vector


text: Concordia University

text: live

text: Generic

text: Complementary DNA was synthesized with ZAP kit (Stratagene) using poly(A)+RNA isolated from Coprinus cinereus cultured under different carbon sources. Synthesis was primed with oligo(dT)/XhoI primer. EcoRI adaptors were ligated to the bunt-ended, double-stranded cDNA. The EcoRI-XhoI-digested cDNA was ligated with EcoRI-XhoI-digested pBluescript KS+ (Invitrogen Corp).

text: E. coli

text: Coprinopsis cinerea

text: XhoI

text: EcoRI

text: Coprinus cinereus pBluescript (EcoRI-XhoI)

text: plasmid

text: pBluescript KS+


text: Concordia University

text: live

text: Generic

text: Complementary DNA was synthesized with ZAP kit (Stratagene) using poly(A)+RNA isolated from Cunninghamella elegans cultured under different carbon sources. Synthesis was primed with oligo(dT)/XhoI primer. EcoRI adaptors were ligated to the bunt-ended, double-stranded cDNA. The EcoRI-XhoI-digested cDNA was ligated with EcoRI-XhoI-digested pBluescript KS+ (Invitrogen Corp).

text: E. coli

text: Cunninghamella elegans

text: XhoI

text: EcoRI

text: Cunninghamella elegans pBluescript (EcoRI-XhoI)

text: plasmid

text: pBluescript KS+


text: Concordia University

text: live

text: Generic

text: Complementary DNA was synthesized with ZAP kit (Stratagene) using poly(A)+RNA isolated from Cryptococcus laurentii cultured under different carbon sources. Synthesis was primed with oligo(dT)/XhoI primer. EcoRI adaptors were ligated to the bunt-ended, double-stranded cDNA. The EcoRI-XhoI-digested cDNA was ligated with EcoRI-XhoI-digested pBluescript KS+ (Invitrogen Corp).

text: E. coli

text: Papiliotrema laurentii

text: XhoI

text: EcoRI

text: Cryptococcus laurentii pBluescript (EcoRI-XhoI)

text: plasmid

text: pBluescript KS+


text: Universite Montpellier2

text: live

text: Generic

text: Spodoptera frugiperda

text: Sf9 Cell Line

characteristic: tissue-type

text: Spodoptera frugiperda cDNA Cell Line Library

text: Plasmid

text: pTriplex2 (Clontech)


text: Leibniz Institute of Plant Biochemistry

text: live

text: Generic

text: Express

characteristic: cultivar

text: Brassica napus

text: buds, flowers, seeds

text: Brassica napus buds, flowers, seeds


text: University of Bath

text: live

text: Generic

text: PolyA RNA from developing seeds were cloned into pSPORT1. Clones were sequenced using either M13 reverse or M13 Forward primers

text: developing seeds from 2-11 Days after pollination

characteristic: dev-stage

text: Arabidopsis arenosa x Arabidopsis thaliana

text: developing seeds

text: Developing seed from A. thaliana (C24) x A. arenosa

text: plasmid


text: Instituto Tecnologico de Celaya, Departamento de Ingenieria Bioquimica

text: live

text: Generic

text: 2 ul PCR fragments were ligated with 1 ul pCR4 -TOPO cloning vector according to the manufacturer instructions (Invitrogen, Carlsbad, CA, USA). 2 ul ligation reaction solutions were transformed into 50 ul E. coli chemically competent cells strain TOP 10. The transformation culture was plated on Petri dishes containing LB/kamamycin, and the screened colony for insert fragment. Selected colonies containing recombinant plasmid were inoculated separately into 5 ml LB/ampicillin/kanamycin solution and it was shaken at 37 oC overnight. Then 500 ul each culture were added into 2 ml cryogenic vial (Corning, Acton, MA, USA) containing 500 ul 100 % glycerol and kept at -80 oC.

text: 45 minutes after interaction with Capsicum annuum in water

text: Phytophthora capsici

text: M13 Forward

text: M13 Reverse

characteristic: strain

text: Mycelium

text: Phytophthora Subtractive Fwd Library

text: Plasmid

text: pCR 4 TOPO TA


text: Concordia University

text: live

text: Generic

text: Complementary DNA was synthesized with ZAP kit (Stratagene) using poly(A)+RNA isolated from Geomyces pannorum cultured under different carbon sources. Synthesis was primed with oligo(dT)/XhoI primer. EcoRI adaptors were ligated to the bunt-ended, double-stranded cDNA. The EcoRI-XhoI-digested cDNA was ligated with EcoRI-XhoI-digested pBluescript KS+ (Invitrogen Corp).

text: E. coli

text: Pseudogymnoascus pannorum

text: XhoI

text: EcoRI

text: Geomyces pannorum pBluescript (EcoRI-XhoI)

text: plasmid

text: pBluescript KS+


text: Concordia University

text: live

text: Generic

text: Complementary DNA was synthesized with ZAP kit (Stratagene) using poly(A)+RNA isolated from Gloeophyllum trabeum cultured under different carbon sources. Synthesis was primed with oligo(dT)/XhoI primer. EcoRI adaptors were ligated to the bunt-ended, double-stranded cDNA. The EcoRI-XhoI-digested cDNA was ligated with EcoRI-XhoI-digested pBluescript KS+ (Invitrogen Corp).

text: E. coli

text: Gloeophyllum trabeum

text: XhoI

text: EcoRI

text: Gloeophyllum trabeum pBluescript (EcoRI-XhoI)

text: plasmid

text: pBluescript KS+


text: Concordia University

text: live

text: Generic

text: Complementary DNA was synthesized with ZAP kit (Stratagene) using poly(A)+RNA isolated from Lentinula edodes cultured under different carbon sources. Synthesis was primed with oligo(dT)/XhoI primer. EcoRI adaptors were ligated to the bunt-ended, double-stranded cDNA. The EcoRI-XhoI-digested cDNA was ligated with EcoRI-XhoI-digested pBluescript KS+ (Invitrogen Corp).

text: E. coli

text: Lentinula edodes

text: XhoI

text: EcoRI

text: Lentinula edodes pBluescript (EcoRI-XhoI)

text: plasmid

text: pBluescript KS+


text: Concordia University

text: live

text: Generic

text: Complementary DNA was synthesized with ZAP kit (Stratagene) using poly(A)+RNA isolated from Leucosporidium scottii cultured under different carbon sources. Synthesis was primed with oligo(dT)/XhoI primer. EcoRI adaptors were ligated to the bunt-ended, double-stranded cDNA. The EcoRI-XhoI-digested cDNA was ligated with EcoRI-XhoI-digested pBluescript KS+ (Invitrogen Corp).

text: E. coli

text: Leucosporidium scottii

text: XhoI

text: EcoRI

text: Leucosporidium scottii pBluescript (EcoRI-XhoI)

text: plasmid

text: pBluescript KS+


text: The Horticulture and Food Research Institute of New Zealand Ltd

text: live

text: Generic

text: Library sequenced by Genesis Research & Development

text: 150 days after full bloom

text: Malus domestica

text: Fruit skin peel

text: (AAFA) Royal Gala apple skin peel, tree-ripened fruit 150 DAFB

text: pBluescript SKC(-)


text: live

text: Generic

text: Seven-week-old seedlings

text: Malus domestica

text: Leaf

text: (ABEA) Royal Gala seedling leaves infected with Venturia inaequalis

text: pBluescript SK(-)


text: Washington University School of Medicine

text: live

text: Generic

text: The library was created by Irina Ronko and Dr. Makedonka Mitreva (mmitreva@watson.wustl.edu) at Washington University, St. Louis using Creator SMART cDNA library construction kit from BD Biosciences. The RNA was provided by Dr. Ralf J. Sommer of Max-Planck Institute, Tuebingen, Germany

text: GC10 cells

text: Pristionchus pacificus

text: SfiI B

text: SfiI A

text: Californian strain PS312

text: plasmid (chloramphenical resistant)

text: pDNR-LIB


text: BC Cancer Agency

text: live

text: Generic

text: 6 months old fetus

text: E. coli DH10B T1 Phage resistant

text: Bos taurus

text: Blunt (5' end of cDNA)

text: NotI (3' end of cDNA)

text: male

characteristic: sex

text: L1 Hereford

text: Skin

text: pExpress 1


text: BC Cancer Agency

text: live

characteristic: INSDC status

text: Generic

text: 6 months old fetus

text: E. coli DH10B T1 Phage resistant

text: Bos taurus

text: Blunt (5' end of cDNA)

text: NotI (3' end of cDNA)

text: male

text: L1 Hereford

text: Muscle

text: pExpress 1


characteristic: External Id

text: Faculdade de Medicina de Ribeirio Preto da Universidade de Sao Paulo

text: live

text: Generic

text: SSH library was constructed following instructions from PCR Select cDNA Subtraction Kit(Clonetech, USA). This library was created to identify T.rubrum up-regulated genes in response to citotoxic compounds exposure.Submitted as Trichophyton rubrum (MYA-3108).

text: Trichophyton rubrum

text: Trichophyton rubrum SSH cDNA Library

text: Plasmid


characteristic: INSDC center name

text: live

text: Generic

text: Adult

text: Petromyzon marinus

text: NotI

text: SalI

text: Plasmid (ampicillin resistant)


text: Radboud University Nijmegen

characteristic: INSDC first public

text: live

text: Generic

text: Blue Bedder

text: Library produced from PCR amplified cDNA from RNA isolated from embryosacs.

text: embryosac

characteristic: organ

text: Petunia x hybrida

text: SfiI

text: SfiI

text: Petunia embryosac SMART cDNA library

text: phagemid


text: Cardiff University

characteristic: INSDC last update

text: live

text: Generic

characteristic: NCBI submission model

text: Total RNA was extracted from halibut larvae, and poly (A)+ RNA purified using oligo-dT cellulose chromatography. cDNA was synthesised and ligated into Eco RI/Xho I digested lambda zap express (Stratagene). The ligated cDNA was packaged into phage particles and amplified, producing a cDNA library

text: Metamorphosis

text: Hippoglossus hippoglossus

text: EcoRI

text: Xho I

text: Male and female

text: Halibut larval cDNA library

text: Phage

text: Lambda zap


text: NRC Institute for Marine Biosciences

text: live

text: Generic

characteristic: NCBI submission package

text: normalised cDNA library generated using the Trimmer Direct kit (Evrogen)

characteristic: description

text: adult

text: DH10B (T1 phage-resistant)

text: gill

text: Hippoglossus hippoglossus

text: SfiI

text: plasmid

text: pDNR-lib


text: NRC Institute for Marine Biosciences

text: live

text: Generic

text: adult

text: DH10B (T1 phage-resistant)

characteristic: lab-host

text: intestine

text: Hippoglossus hippoglossus

characteristic: organism

text: SfiI

text: plasmid

text: pDNR-lib


text: NRC Institute for Marine Biosciences

text: live

text: Generic

text: larvae 2 (mouth-opening)

text: DH10B (T1 phage-resistant)

text: Hippoglossus hippoglossus

text: SfiI

characteristic: title

text: plasmid

text: pDNR-lib

characteristic: vector


text: NRC Institute for Marine Biosciences

text: live

text: Generic

text: larvae 3 (midway to metamorphosis)

text: DH10B (T1 phage-resistant)

text: Hippoglossus hippoglossus

text: SfiI

text: plasmid

text: pDNR-lib


text: NRC Institute for Marine Biosciences

text: live

text: Generic

text: larvae 4 (premetamorphosis)

text: DH10B (T1 phage-resistant)

text: Hippoglossus hippoglossus

text: SfiI

text: plasmid

text: pDNR-lib


text: NRC Institute for Marine Biosciences

text: live

text: Generic

text: larvae 5 (postmetamorphosis)

text: DH10B (T1 phage-resistant)

text: Hippoglossus hippoglossus

text: SfiI

text: plasmid

text: pDNR-lib


text: NRC Institute for Marine Biosciences

text: live

text: Generic

text: adult

text: DH10B (T1 phage-resistant)

text: liver

text: Hippoglossus hippoglossus

text: SfiI

text: plasmid

text: pDNR-lib


text: NRC Institute for Marine Biosciences

text: live

text: Generic

text: adult

text: DH10B (T1 phage-resistant)

text: ovary

text: Hippoglossus hippoglossus

text: SfiI

text: plasmid

text: pDNR-lib


text: NRC Institute for Marine Biosciences

text: live

text: Generic

text: adult

text: DH10B (T1 phage-resistant)

text: skin

text: Hippoglossus hippoglossus

text: SfiI

text: plasmid

text: pDNR-lib


text: NRC Institute for Marine Biosciences

text: live

text: Generic

text: adult

text: DH10B (T1 phage-resistant)

text: spleen

text: Hippoglossus hippoglossus

text: SfiI

text: plasmid

text: pDNR-lib


text: NRC Institute for Marine Biosciences

text: live

text: Generic

text: adult

text: DH10B (T1 phage-resistant)

text: testis

text: Hippoglossus hippoglossus

text: SfiI

text: male

text: plasmid

text: pDNR-lib


text: Nanjing Agricultural University

text: live

text: Generic

text: AiJiaoHuang

characteristic: cultivar

text: suppression subtractive hybridization library

text: Brassica rapa subsp. chinensis

text: SSH library of non-heading Chinese cabbage genes up-regulated in early response to elicitor PB90


text: Sun Yat-Sen University

text: live

text: Generic

text: Nicotiana tabacum

text: leaf

characteristic: tissue-type

text: Tobacco Leaves Express Library


text: Life Sciences School, SOOCHOW University

text: live

text: Generic

text: T cell

characteristic: cell type

text: These sequences obtained by SSH and the populations of gene fragments generated by subtractive hybridizations are differentially expressed in human T cells.

text: adult

characteristic: dev-stage

text: Homo sapiens

text: male

text: T cell activated by anti-CD3 and anti-CD28 Subtracted with the normal human T cell


text: Concordia University

text: live

characteristic: INSDC status

text: Generic

text: Complementary DNA was synthesized with ZAP kit (Stratagene) using poly(A)+RNA isolated from Ophiostoma piliferum cultured under different carbon sources. Synthesis was primed with oligo(dT)/XhoI primer. EcoRI adaptors were ligated to the bunt-ended, double-stranded cDNA. The EcoRI-XhoI-digested cDNA was ligated with EcoRI-XhoI-digested pBluescript KS+ (Invitrogen Corp).

text: E. coli

text: Ophiostoma piliferum

text: XhoI

text: EcoRI

text: Ophiostoma piliferum pBluescript (EcoRI-XhoI)

text: plasmid

text: pBluescript KS+


characteristic: External Id

text: Concordia University

text: live

text: Generic

text: Complementary DNA was synthesized with ZAP kit (Stratagene) using poly(A)+RNA isolated from Sporotrichum thermophile cultured under different carbon sources. Synthesis was primed with oligo(dT)/XhoI primer. EcoRI adaptors were ligated to the bunt-ended, double-stranded cDNA. The EcoRI-XhoI-digested cDNA was ligated with EcoRI-XhoI-digested pBluescript KS+ (Invitrogen Corp).

text: E. coli

text: Thermothelomyces heterothallicus

text: XhoI

text: EcoRI

text: Sporotrichum thermophile pBluescript (EcoRI-XhoI)

text: plasmid

text: pBluescript KS+


text: Concordia University

characteristic: INSDC center name

text: live

text: Generic

text: Complementary DNA was synthesized with ZAP kit (Stratagene) using poly(A)+RNA isolated from Thermomyces lanuginosa cultured under different carbon sources. Synthesis was primed with oligo(dT)/XhoI primer. EcoRI adaptors were ligated to the bunt-ended, double-stranded cDNA. The EcoRI-XhoI-digested cDNA was ligated with EcoRI-XhoI-digested pBluescript KS+ (Invitrogen Corp).

text: E. coli

text: Thermomyces lanuginosus

text: XhoI

text: EcoRI

text: Thermomyces lanuginosa pBluescript (EcoRI-XhoI)

text: plasmid

text: pBluescript KS+


text: Concordia University

characteristic: INSDC first public

text: live

text: Generic

text: Complementary DNA was synthesized with ZAP kit (Stratagene) using poly(A)+RNA isolated from Trametes versicolor cultured under different carbon sources. Synthesis was primed with oligo(dT)/XhoI primer. EcoRI adaptors were ligated to the bunt-ended, double-stranded cDNA. The EcoRI-XhoI-digested cDNA was ligated with EcoRI-XhoI-digested pBluescript KS+ (Invitrogen Corp).

text: E. coli

text: Trametes versicolor

text: XhoI

text: EcoRI

text: Trametes versicolor pBluescript (EcoRI-XhoI)

text: plasmid

text: pBluescript KS+


text: The University of Melbourne

text: live

text: Generic

text: adult

text: Oesophagostomum dentatum

text: gender-enriched suppressive-subtractive hybridised library


text: University of Aberdeen

text: live

text: Generic

text: E. coli TAM1

text: Oncorhynchus mykiss

text: kidney

text: Rainbow trout head kidney SSH library from infection (rtay)

text: pGEM-T easy


text: University of Aberdeen

text: live

text: Generic

text: E. coli TAM1

text: Oncorhynchus mykiss

text: liver

text: Rainbow trout liver SSH library from bacterial challenge (rtal)

text: pGEM-T easy


text: University of Aberdeen

text: live

text: Generic

text: E. coli TAM1

text: Oncorhynchus mykiss

text: gill

text: Rainbow trout gill SSH library from bacterial challenge (rtag)

text: pGEM-T easy


text: University of Aberdeen

text: live

text: Generic

text: E. coli TAM1

text: Oncorhynchus mykiss

text: spleen

text: Rainbow trout spleen SSH library from infection (rtas)

text: pGEM-T easy


text: University of Aberdeen

text: live

text: Generic

text: E. coli TAM1

text: Oncorhynchus mykiss

text: multi-tissues

text: Rainbow trout multi-tissues SSH library from infection (rtax)

text: pGEM-T easy


text: University of Aberdeen

text: live

text: Generic

text: E. coli TAM1

text: Oncorhynchus mykiss

text: gut

text: Rainbow trout intestine SSH library from bacterial challenge (rtai)

text: pGEM-T easy


text: NIAST, RDA, Department of Agricultural Environment

text: live

text: Generic

text: Korean native strain

text: 24hr after oviposition

text: Bombyx mori

text: EcoRI

text: NotI

characteristic: strain

text: fertilized egg

text: Bombyx mori embryo cDNA Library

text: pCNS


text: National Institute of Radiological Sciences

characteristic: INSDC last update

text: live

text: Generic

text: fibroblast

text: HFLIII

characteristic: cell-line

text: Homo sapiens

text: Homo sapiens HFLIII


text: Forestry and Forest Products Research Institute

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Cryptomeria japonica

text: xylem

text: Cryptomeria japonica supression subtracted cDNA library


text: The Institute of Physical and Chemical Research (RIKEN)

text: live

text: Generic

text: B cells

text: CRL-1669 BCL1 Clone 13.20-3B3

text: Mus musculus

text: BALB/c

text: RIKEN full-length enriched mouse cDNA library, BALB/c CRL-1669 BCL1 Clone 13.20-3B3 B cells


text: live

text: Generic

text: TIB-55 BB88

text: Mus musculus

text: BALB/c

text: RIKEN full-length enriched mouse cDNA library, BALB/c TIB-55 BB88


text: Japan Women's University

text: live

text: Generic

text: conjugation-induced cells by the mixing of both types of cells (0, 2, 4, 8, 12, 24, 48, and 72h after the mixing) and vegetatively grown cells

characteristic: description

text: Closterium peracerosum-strigosum-littorale complex

text: NIES-67 and NIES-68

text: cDNA from vegetative and sexual reproductive stages of Closterium


text: The Institute for Genomic Research

text: live

text: Generic

text: This library was constructed by Wayne Hunter and Phat Dang (U. S. Horticultural Research Laboratory, 2001 South Rock Road, Fort Pierce, FL 34945, USA) using the Stratagene cDNA library kit. Total RNA was extracted using Qiagen RNA extraction kits, DNAse Treated (Promega), single pass Poly(A) isolated (Ambion). cDNAs were cloned into phage, packaged, and titered with primary library consisting greater than 1 X 10^7 pfu/mL. Phage libraries were amplified, and mass-excised for library sequencing. The mass-excised libraries (pBlueScript SK) phagemids were reinfected into MRF' XLI Blue cells.

text: Pupal-adult

text: Nasonia giraulti

characteristic: organism

text: RV2 (Wolbachia infected)

text: Nasonia giraulti (wasp) pupal-adult EST library

text: Plasmid

text: pBluescript SK(-)


text: The Institute for Genomic Research

text: live

text: Generic

text: Pre-pupal

text: Nasonia giraulti

text: RV2 (Wolbachia infected)

text: Nasonia giraulti (wasp) pre-pupal EST library

characteristic: title

text: Plasmid

text: pBluescript SK(-)


text: The Institute for Genomic Research

text: live

text: Generic

text: Pupal-adult

text: Nasonia vitripennis

text: AsymCx(lbii)

text: Nasonia vitripennis (wasp) pupal-adult EST library

text: Plasmid

text: pBluescript SK(-)

characteristic: vector


text: The Institute for Genomic Research

text: live

text: Generic

text: Pre-pupal

text: Nasonia vitripennis

text: AsymCx(lbii)

text: Nasonia vitripennis (wasp) pre-pupal EST library

text: Plasmid

text: pBluescript SK(-)


text: The Institute for Genomic Research

text: live

text: Generic

text: This library was constructed by David Leaf (Biology Department, Western Washington University, Bellingham, WA 98225, USA)

text: 0 to 24 hours of development

text: Nasonia vitripennis

text: Nasonia vitripennis (wasp) 0-24h EST library

text: Plasmid

text: pDNR-lib


text: Simon Fraser University

text: live

text: Generic

text: Rhodnius prolixus midgut subtracted library in response to bacterial (E. coli and M. luteus) injection (24h pi).

text: Adult

text: Rhodnius prolixus

text: Adult midgut

text: Midgut subtracted library in response to bacterial injection


text: Simon Fraser University

text: live

text: Generic

text: Rhodnius prolixus fat body subtracted library in response to bacterial (E. coli and M. luteus) injection (24h pi).

text: Adult

text: Rhodnius prolixus

text: Adult fat body

text: Fat body subtracted library in response to bacterial injection


text: Simon Fraser University

text: live

text: Generic

text: Rhodnius prolixus fat body subtracted library in response to Trypanosoma cruzi injection (24h pi).

text: Adult

text: Rhodnius prolixus

text: Adult fat body

text: Fat body subtracted library in response to Trypanosoma cruzi injection


text: The Institute of Physical and Chemical Research (RIKEN)

text: live

text: Generic

text: Homo sapiens

text: hippocampus

text: RIKEN full-length enriched human cDNA library, hippocampus


text: live

text: Generic

text: Homo sapiens

text: testis

text: RIKEN full-length enriched human cDNA library, testis


text: live

text: Generic

text: Homo sapiens

text: hypothalamus

text: RIKEN full-length enriched human cDNA library, hypothalamus


text: INRA

text: live

text: Generic

text: cDNA-AFLP transcript-derived fragments expressed in potato roots inoculated with Globodera pallida nematode

text: root

characteristic: organ

text: Solanum sparsipilum x Solanum tuberosum

text: TDFs expressed in potato x nematode interaction


text: Universidade Federal de Goias

text: live

text: Generic

text: Paracoccidioides brasiliensis

text: Paracoccidioides brasiliensis pGem-T Easy library

text: Plasmid


text: University of Georgia

text: live

text: Generic

characteristic: cultivar

text: Eleusine coracana subsp. africana

text: Root

characteristic: tissue-type

text: Finger millet pTriplEx2 library 3

text: Lambda TriplEx2


text: National Cancer Institute / NIH

text: live

text: Generic

text: cDNA was primed using oligo-dT primer: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3' and cloned into the EcoRV/NotI sites of pExpress-1. Size-selection 1.2kb resulted in an average insert size of 1.9kb. This is a non-normalized primary library and was constructed by Express Genomics (Frederick, MD) for the (http://xgc.nci.nih.gov/) Xenopus Gene Collection.

text: DH10B TonA

characteristic: lab-host

text: spleen

text: Xenopus laevis

text: EcoRV

text: NotI

text: plasmid


text: National Cancer Institute / NIH

text: live

characteristic: INSDC status

text: Generic

text: cDNA was primed using oligo-dT primer: GACTAGTTCTAGATCGCGAGCGGCCGCC(T)25 and cloned into the SmaI/NotI sites of pCS111. Size-selection >1.25kb resulted in an average insert size of 1.9 kb. This primary library was constructed by Express Genomics (Frederick, MD). Note: this is a (http://xgc.nci.nih.gov/) Xenopus Gene Collection library.

text: DH10B TonA

text: embryo

text: Xenopus laevis

text: SmaI

text: NotI

text: whole embryo - stages 56-62

text: plasmid


characteristic: External Id

text: National Cancer Institute / NIH

text: live

text: Generic

text: cDNA was primed using oligo-dT primer: GACTAGTTCTAGATCGCGAGCGGCCGCC(T)25 and cloned into the SmaI/NotI sites of pCS111. Size-selection >1.25 kb resulted in an average insert size of 1.6 kb. This primary library was constructed by Express Genomics (Frederick, MD). Note: this is a (http://xgc.nci.nih.gov/) Xenopus Gene Collection library.

text: DH10B TonA

text: limb

text: Xenopus laevis

text: SmaI

text: NotI

text: forelimbs and hindlimbs, pooled stages 54-61

text: plasmid


text: National Cancer Institute / NIH

characteristic: INSDC center name

text: live

text: Generic

text: cDNA was primed using oligo-dT primer: GACTAGTTCTAGATCGCGAGCGGCCGCC(T)25 and cloned into the SmaI/NotI sites of pCS111. Size-selection >1.25 kb resulted in an average insert size of 1.8 kb. This primary library was constructed by Express Genomics (Frederick, MD). Note: this is a (http://xgc.nci.nih.gov/) Xenopus Gene Collection library.

text: DH10B TonA

text: embryo

text: Xenopus laevis

text: SmaI

text: NotI

text: tails, stage 56-62

text: plasmid


text: Martek Biosciences Corp

characteristic: INSDC first public

text: live

text: Generic

text: Crypthecodinium cohnii

text: C. cohnii pExpress-1 Library

text: plasmid

text: pExpress-1 vector


text: Ludwig-Maximilians University Muenchen

text: live

text: Generic

text: Subtracted cDNA library; SSH method; enriched for cDNAs up-regulated at day 18 of pregnancy after artificial insemination compared to day 18 of estrous cycle (insemination with seminal plasma) in bovine endometrium

text: day 18 of pregnancy

characteristic: dev-stage

text: E. coli SURE

text: uterus

text: Bos taurus

text: BssHII

text: BssHII

text: female

characteristic: sex

text: Simmental

characteristic: strain

text: endometrium

text: Bovine SSH library; endometrium; day 18 of pregnancy; 18KB

text: Plasmid

text: derivative of pBSIISK(-)


text: Ludwig-Maximilians University Muenchen

characteristic: INSDC last update

text: live

text: Generic

characteristic: NCBI submission model

text: Subtracted cDNA library; SSH method; enriched for cDNAs up-regulated at day 18 of estrous cycle (insemination with seminal plasma) compared to day 18 of pregnancy after artificial insemination in bovine endometrium

text: day 18 of estrous cycle

text: E. coli SURE

text: uterus

text: Bos taurus

text: BssHII

text: BssHII

text: female

text: Simmental

text: endometrium

text: Bovine SSH library; endometrium; day 18 of estrous cycle; 18KO

text: Plasmid

text: derivative of pBSIISK(-)


text: Faculdade de Medicina de Ribeirio Preto da Universidade de Sao Paulo

text: live

text: Generic

characteristic: NCBI submission package

text: SSH library was constructed following instructions from PCR Select cDNA Subtraction Kit(Clonetech, USA). This library was created to identify T.rubrum up-regulated genes during keratin assimilation.

characteristic: description

text: E.coli MOS Blue

text: Trichophyton rubrum

text: Trichophyton rubrum SSH cDNA Library pGEM-T

text: Plasmid

text: pGEM-T


text: The Horticulture and Food Research Institute of New Zealand Ltd

text: live

text: Generic

text: Library sequenced by Genesis Research & Development

text: 126 days after full bloom

text: Malus domestica

characteristic: organism

text: fruit

text: (AABA) Royal Gala 126 DAFB fruit core (same tissue as AAXA library)

characteristic: title

text: pBK-CMV


text: live

text: Generic

text: Seven-week-old seedlings

text: Malus domestica

text: Leaf

text: (ABEB) Royal Gala seedling leaves infected with Venturia inaequalis

text: pBluescript SK(-)

characteristic: vector


text: Southwest Hospital, Third Military Medical University

text: live

text: Generic

text: This EST fragment was amplified from Candida albicans cDNA Library with GLGI technique (Generation of Longer cDNA fragments from SAGE tags for Gene Identification, Proc. Natl. Acad. Sci. USA 97, 349, 2000), which starts from the 3' end till the last CATG site of the target cDNA sequence.

text: Candida albicans

text: Candida albicans cDNA library


text: University of St Andrews

text: live

text: Generic

text: The library was prepared by Dr. Jialin Bai, Fish Muscle Research Group, Gatty Marine Laboratory, School of Biology, University of St Andrews.

text: 1 day-old yolk-sac

text: Hippoglossus hippoglossus

text: EcoR I

text: Xho I

text: larvae(trunk)

text: 1 day-old yolk-sac larvae(trunk) cDNA library of Atlantic halibut, Hippoglossus hippoglossus

text: plasmid

text: pBlueScript II SK(+) XR


text: Rajiv Gandhi Centre for Biotechnology

text: live

text: Generic

text: Piper colubrinum

text: RsaI

text: RsaI

text: Monoecious

text: Leaf

text: Piper colubrinum, salicylic acid induced, subtracted, clontech (K 1804-1) (RsaI)

text: Plasmid

text: pGEMT-Easy


text: Northeast Forestry University

text: live

text: Generic

text: Suppression subtractive hybridization cDNA Library

text: Puccinellia tenuiflora

text: leaf

text: SSH cDNA Library of Puccinellia tenuiflora treated with NaHCO3 for 12h


text: Northeast Forestry University

text: live

text: Generic

text: Puccinellia tenuiflora

text: leaf

text: SSH cDNA Library of Puccinellia tenuiflora treated with NaHCO3 for 24h


text: Northeast Forestry University

text: live

text: Generic

text: Puccinellia tenuiflora

text: leaf

text: SSH cDNA Library of Puccinellia tenuiflora treated with NaHCO3 for 48h


text: Northeast Forestry University

text: live

text: Generic

text: Puccinellia tenuiflora

text: leaf

text: SSH cDNA Library of Puccinellia tenuiflora treated with NaHCO3 for 6h


text: Nantong Medical college

text: live

text: Generic

text: Gekko japonicus

text: injuried spinal cord

text: injuried spinal cord cDNA library in Gecko


text: University of St Andrews

text: live

text: Generic

text: juvenile(0.42 kg body mass)

text: Hippoglossus hippoglossus

text: EcoR I

text: Xho I

text: fast myotomal muscle

text: cDNA library of fast myotomal muscle(juvenile, 0.42kg body mass) of Atlantic halibut, Hippoglossus hippoglossus

text: plasmid

text: pBlueScript II SK(+) XR


text: Institute of Evolution University of Haifa

text: live

text: Generic

text: Hordeum vulgare subsp. spontaneum

text: Seedling shoot

text: Hordeum vulgare subsp. spontaneum cDNA-AFLP library, dehydration stress


text: Vrije Universiteit Amsterdam

text: live

text: Generic

characteristic: cultivar

text: supplier: Petunia x hybrida cv. R27 Diploid plants. RNA was extracted from dissected apices.

text: Petunia x hybrida

text: apices

text: apices cDNA library

text: UNIZAP XR


text: Vrije Universiteit Amsterdam

text: live

text: Generic

text: supplier: Petunia x hybrida cv. W138 Diploid plants. RNA was extracted from dissected inflorescences

text: Petunia x hybrida

text: inflorescence

characteristic: tissue-type

text: meristem Y2H cDNA library


text: Vrije Universiteit Amsterdam

text: live

text: Generic

text: supplier: Petunia x hybrida cv. R27 Diploid plants.

text: Petunia x hybrida

text: different organs

text: mixed organs cDNA library

text: UNIZAP XR


text: Vrije Universiteit Amsterdam

text: live

text: Generic

text: supplier: Petunia x hybrida cv. R27 Diploid plants. RNA was extracted from petal of buds at maximum elongation.

text: Petunia x hybrida

text: petals

text: petals cDNA library

text: HYBRIZAP XR


text: Vrije Universiteit Amsterdam

text: live

text: Generic

text: R27 and W225

text: supplier: Petunia x hybrida cv. R27 and W225 diploid plants. RNA was extracted from petal of buds at maximum elongation. cDNA from wild type buds (R27) was subtracted (hybrid select) with cDNA from an1 mutant buds (W225)

text: Petunia x hybrida

text: petals

text: petals cDNA subtraction (hybrid select)

text: pBS KS (+)


text: Vrije Universiteit Amsterdam

text: live

text: Generic

text: Petunia x hybrida

text: petals

text: petals Y2H cDNA library

text: HYBRIZAP 2.1


text: Vrije Universiteit Amsterdam

text: live

text: Generic

text: Petunia x hybrida

text: petals

text: petals cDNA AFLP


text: Washington University School of Medicine

text: live

text: Generic

text: pDNR-LIB; Site_1: Sfi IB (5'-GCCTC-3'); Site_2: Sfi IA (5'-ATTAC-3'); Parasitic second-stage juveniles of G. rostochiensis were collected 11 days after inoculation of potato tubers with cysts of G.rostochiensis line 19 (avirulent) by homogenizing the whole root system and purifying juveniles by sieving and centrifugation in sucros gradient. Total RNA was isolated according to TRIzol protocol, followed by mRNA isolation (Dynabeads mRNA purification Kit, Dynal). mRNA was used in first strand cDNA synthesis according to the Creator SMART cDNA library construction protocol, followed by cDNA amplification by 22 cycles of Long-Distance (LD) PCR, SfiI digestion, cDNA size fractionation, ligation into pDNR-LIB vector and transformation into electrocompetent TOP10 E. coli. This library was contributed by Anna Tomczak and Dr. Geert Smant (Geert.Smant@wur.nl) of the Laboratory of Nematology at Wageningen University, Wageningen, Netherlands.

text: J2 parasitic stage, 11 days post inoculation with cysts

characteristic: lab-host

text: Globodera rostochiensis

text: SfiI B

text: SfiI A

text: line 19

text: plasmid

text: pDNR-LIB


text: live

characteristic: INSDC status

text: Generic

text: The library was constructed by Irina Ronko and Dr. Makedonka Mitreva at Washington University, St. Louis. Oligo(dT)-SL1 PCR based library. Nippostrongylus brasiliensis activated L3 cDNA PCR products of size >400 nucleotides containing SL1 on the 5' end and oligo(dT) on the 3' end were non-directionally cloned into pCRII-TOPO(Invitrogen) following the Topo TA cloning protocol. RNA was provided by Dr. Murray E. Selkirk (m.selkirk@imperial.ac.uk) of Imperial College London, UK.

text: L3, activated

text: GC10 cells

text: Nippostrongylus brasiliensis

text: EcoRI

text: EcoRI

text: Plasmid

text: pCRII-TOPO


characteristic: External Id

text: live

text: Generic

text: The library was constructed by Irina Ronko and Dr. Makedonka Mitreva at Washington University, St. Louis. Oligo(dT)-SL1 PCR based library. Nippostrongylus brasiliensis L3 cDNA PCR products of size >400 nucleotides containing SL1 on the 5' end and oligo(dT) on the 3' end were non-directionally cloned into pCRII-TOPO(Invitrogen) following the Topo TA cloning protocol. RNA was provided by Dr. Murray E. Selkirk (m.selkirk@imperial.ac.uk) of Imperial College London, UK.

text: GC10 cells

text: Nippostrongylus brasiliensis

text: EcoRI

text: EcoRI

text: Plasmid

text: pCRII-TOPO


text: University of Georgia

characteristic: INSDC center name

text: live

text: Generic

text: IE 1012

text: Eleusine coracana subsp. coracana

text: Inflorescence

text: Finger millet pTriplEx2 library 4

text: Lambda TriplEx2


text: Columbia University

characteristic: INSDC first public

text: live

text: Generic

text: Aplysia californica

text: NotI

text: EcoRI

text: Pedal-Pleural ganglia

text: Non-Normalized Pedal-Pleural Ganglia

text: Plasmid

text: Pbluescript SKII+


text: Columbia University

text: live

text: Generic

text: PEG002 and PEG003

text: Aplysia californica

text: NotI

text: EcoRI

text: Pedal-Pleural ganglia

text: Normalized Pedal-Pleural Ganglia

text: Plasmid

text: Pbluescript SKII+


text: University of Florida

text: live

text: Generic

text: Neuronal processes of MCC (metacerebral neuron)

characteristic: cell type

text: Aplysia californica

text: 5'- CGA CGT GGA CTA TCC ATG AAC

text: 5'- CGC AGT CGG TAC (T)13 -3' (T

text: Cerebral ganglion

text: Non-Normalized MCC Processes

text: Plasmid

text: pGemT


text: University of Florida

text: live

text: Generic

text: Aplysia californica

text: 5'- CGA CGT GGA CTA TCC ATG AAC

text: 5'- CGC AGT CGG TAC (T)13 -3' (T

text: Cerebral ganglion

text: Non-Normalized MCC Processes 2

text: Plasmid

text: pGemT


text: University of Florida

text: live

text: Generic

text: Left and right MCC (metacerebral neuron)

text: Aplysia californica

text: 5'- CGA CGT GGA CTA TCC ATG AAC

text: 5'- CGC AGT CGG TAC (T)13 -3' (T

text: Cerebral ganglion

text: Non-Normalized MCC Neurons 1

text: Plasmid

text: pGemT


text: University of Florida

text: live

text: Generic

text: MCC (metacerebral neuron)

text: Aplysia californica

text: 5'- CGA CGT GGA CTA TCC ATG AAC

text: 5'- CGC AGT CGG TAC (T)13 -3' (T

text: Cerebral ganglion

text: Non-Normalized MCC Neurons 2

text: Plasmid

text: pGemT


text: University of Florida

text: live

text: Generic

text: mT 3'seq)MCCN20

text: Aplysia californica

text: 5'- TAT CAA CGC AGC CGA CCT CTC

text: 5'- CGC AGT CGG TAC (T)13 -3' (C

text: Cerebral gannglion

text: Normalized MCC Neurons

text: Plasmid

text: pGemT


text: Okayama University, Barley Germplasm Center

text: live

text: Generic

text: Haruna Nijo

text: Seedling

characteristic: dev-stage

text: Hordeum vulgare subsp. vulgare

text: Shoot

text: Etiolated seedling shoot


text: live

text: Generic

text: Haruna Nijo

text: Germination

text: Hordeum vulgare subsp. vulgare

text: Shoot

text: Germination shoots


text: live

text: Generic

text: Haruna Nijo

text: Germination

text: Hordeum vulgare subsp. vulgare

text: Root

text: Seminal root


text: Northeast Forestry University

text: live

text: Generic

text: Tamarix sp. ZDY-001908 (Tamarix albiflora ined.); Suppression subtractive hybridization cDNA Library

text: Tamarix sp. ZDY-001908

text: LEAF

text: SSH cDNA Library of Tamarix albiflonum under drought stress


text: University of Florida

text: live

text: Generic

text: Neuronal processes of MCC (metacerebral neurons)

text: mT 3'seq)MCCN24

text: Aplysia californica

text: 5'- TAT CAA CGC AGC CGA CCT CTC

text: 5'- CGC AGT CGG TAC (T)13 -3' (C

text: Cerebral ganglion

text: Normalized MCC Processes 2

text: Plasmid

text: pGemT


text: University of Florida

text: live

text: Generic

text: mT 3'seq)MCCN25

text: Aplysia californica

text: 5'- TAT CAA CGC AGC CGA CCT CTC

text: 5'- CGC AGT CGG TAC (T)13 -3' (C

text: Cerebral ganglion

text: Normalized MCC Processes 1

text: Plasmid

text: pGemT


text: University of Florida

text: live

text: Generic

text: mT 3'seq)CNSN01

text: juvenile

text: Aplysia californica

text: 5'- TAT CAA CGC AGC CGA CCT CTC

text: 5'- CGC AGT CGG TAC (T)13 -3' (C

text: Central nervous system (all ganglia)

text: Normalized CNS library (juvenile 1)

text: Plasmid

text: pGemT


text: University of Florida

text: live

text: Generic

text: mT 3'seq)CNSN02

text: juvenile

text: Aplysia californica

text: 5'- TAT CAA CGC AGC CGA CCT CTC

text: 5'- CGC AGT CGG TAC (T)13 -3' (C

text: CNS

text: Normalized CNS library (juvenile 2)

text: Plasmid

text: pGemT


text: University of Florida

text: live

text: Generic

text: mT 3'seq)CNSN23

text: adult

text: Aplysia californica

text: 5'- TAT CAA CGC AGC CGA CCT CTC

text: 5'- CGC AGT CGG TAC (T)13 -3' (C

text: CNS

text: Normalized CNS library (adult 2)

text: Plasmid

text: pGemT


text: live

text: Generic

text: mT 3'seq)CNSN24

text: adult

text: Aplysia californica

text: 5'- TAT CAA CGC AGC CGA CCT CTC

text: 5'- CGC AGT CGG TAC (T)13 -3' (C

text: CNS

text: Normalized CNS library (adult 3)

text: Plasmid

text: pGemT


text: live

text: Generic

text: mT 3'seq)CNSN25

text: juvenile

text: Aplysia californica

text: 5'- TAT CAA CGC AGC CGA CCT CTC

text: 5'- CGC AGT CGG TAC (T)13 -3' (C

text: CNS

text: Normalized CNS library (juvenile 3)

text: Plasmid

text: pGemT


text: University of Florida

text: live

text: Generic

text: mT 3'seq)CNSN27

text: adult

text: Aplysia californica

text: 5'- TAT CAA CGC AGC CGA CCT CTC

text: 5'- CGC AGT CGG TAC (T)13 -3' (C

text: CNS

text: Normalized CNS library (adult 1)

text: Plasmid

text: pGemT


text: University of Florida

text: live

text: Generic

text: a)CNSnN

text: adult

text: Aplysia californica

text: 5'- CGA CGT GGA CTA TCC ATG AAC

text: 5'- CGC AGT CGG TAC (T)13 -3' (T

text: CNS

text: Non-Normalized CNS library

text: Plasmid

text: pGemT


text: California Institute of Technology

text: live

text: Generic

text: Immature T-cells

text: The cDNA library is the one used in the following publication: Anderson, M.K., Et. al. (1999). Precise Developmental Regulation of Ets Family Transcription Factors During Specification and Commitment to the T-Cell Lineage. Development 126, 3131-3148.

text: Young adult

text: E. Coli

text: Thymus

characteristic: organ

text: Mus musculus

characteristic: strain

text: SCID Mouse Thymus Regulatory Genes

text: Plasmid

text: pSPORT 1


characteristic: INSDC last update

text: live

text: Generic

characteristic: NCBI submission model

text: Clarias macrocephalus

text: muscle

text: Walking catfish muscle library


text: live

text: Generic

characteristic: NCBI submission package

text: liver

text: Clarias macrocephalus

text: liver

text: Walking catfish liver library


text: National Eye Institute

text: live

text: Generic

text: RNA was extracted from lenses from two human embryos 45 and 46 days old. cDNA was cloned in XbaI/BamHI sites in the pcDNA3.1- vector. EST analysis was performed at the NIH Intramural Sequencing Center (NISC). Analyzed data available through http://neibank.nei.nih.gov.

characteristic: description

text: Fetal

text: Eye

text: Homo sapiens

characteristic: organism

text: Lens

text: Human fetal lens. (ki)

text: Plasmid


text: National Eye Institute

text: live

text: Generic

text: RNA was extracted from pooled guinea pig retinas. A directionally cloned cDNA library in the pCMVSPORT6 vector (Invitrogen) was constructed at Bioserve Biotechnology (Laurel MD) essentially following the protocols of the SuperScript Plasmid System, full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. cDNA was cloned in Not I/Sal I sites. EST analysis was performed at the NIH Intramural Sequencing Center (NISC). Analyzed data available through http://neibank.nei.nih.gov.

text: adult

text: Eye

text: Cavia porcellus

text: Retina

text: Guinea pig retina. Unnormalized (naz)

characteristic: title

text: Plasmid

characteristic: vector


text: National Eye Institute

text: live

text: Generic

text: RNA was extracted from pooled guinea pig eye minus retina and lens. A directionally cloned cDNA library in the pCMVSPORT6 vector (Invitrogen) was constructed at Bioserve Biotechnology (Laurel MD) essentially following the protocols of the SuperScript Plasmid System, full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. cDNA was cloned in Not I/Sal I sites. EST analysis was performed at the NIH Intramural Sequencing Center (NISC). Analyzed data available through http://neibank.nei.nih.gov.

text: adult

text: Eye

text: Cavia porcellus

text: Eye minus retina and lens

characteristic: tissue-type

text: Guinea pig eye minus retina and lens. Unnormalized (nba)

text: Plasmid


text: National Eye Institute

text: live

text: Generic

text: RNA was extracted from pooled guinea pig lens. A directionally cloned cDNA library in the pCMVSPORT6 vector (Invitrogen) was constructed at Bioserve Biotechnology (Laurel MD) essentially following the protocols of the SuperScript Plasmid System, full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. cDNA was cloned in Not I/Sal I sites. EST analysis was performed at the NIH Intramural Sequencing Center (NISC). Analyzed data available through http://neibank.nei.nih.gov.

text: adult

characteristic: lab-host

text: Eye

text: Cavia porcellus

text: Lens

text: Guinea pig lens. Unnormalized (nbb)

text: Plasmid


text: National Eye Institute

text: live

characteristic: INSDC status

text: Generic

text: RNA was extracted from pooled rabbit trigeminal nerve. A directionally cloned cDNA library in the pCMVSPORT6 vector (Invitrogen) was constructed at Bioserve Biotechnology (Laurel MD) essentially following the protocols of the SuperScript Plasmid System, full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. cDNA was cloned in Not I/Sal I sites. EST analysis was performed at the NIH Intramural Sequencing Center (NISC). Analyzed data available through http://neibank.nei.nih.gov.

text: adult

text: Oryctolagus cuniculus

text: Trigeminal nerve

text: Rabbit trigeminal nerve. Unnormalized (nbc)

text: Plasmid


characteristic: External Id

text: National Eye Institute

text: live

text: Generic

text: RNA was extracted from pooled rabbit lens. A directionally cloned cDNA library in the pCMVSPORT6 vector (Invitrogen) was constructed at Bioserve Biotechnology (Laurel MD) essentially following the protocols of the SuperScript Plasmid System, full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. cDNA was cloned in Not I/Sal I sites. EST analysis was performed at the NIH Intramural Sequencing Center (NISC). Analyzed data available through http://neibank.nei.nih.gov.

text: adult

text: Eye

text: Oryctolagus cuniculus

text: Lens

text: Rabbit lens. Unnormalized (nbd)

text: Plasmid


text: National Eye Institute

characteristic: INSDC center name

text: live

text: Generic

text: RNA was extracted from pooled human optic nerve. A directionally cloned cDNA library in the pCMVSPORT6 vector (Invitrogen) was constructed at Bioserve Biotechnology (Laurel MD) essentially following the protocols of the SuperScript Plasmid System, full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. cDNA was cloned in Not I/Sal I sites. EST analysis was performed at the NIH Intramural Sequencing Center (NISC). Analyzed data available through http://neibank.nei.nih.gov.

text: adult

text: Eye

text: Homo sapiens

text: Optic nerve

text: Human optic nerve. Unnormalized (nbj)

text: Plasmid


text: Central Institute of Medicinal and Aromatic Plants

characteristic: INSDC first public

text: live

text: Generic

text: Whole capsule wall

text: cDNA populations derived from the subtractive hybridization of highly alkaloid producing capsule and low alkaloid producing capsule wall tissues of Papaver somniferum L. The high alkaloid producing specific messages were cloned in the pGEM-T vector.

text: E coli

text: capsule

text: Papaver somniferum

text: Hermaphrodite

characteristic: sex

text: Capsule wall

text: Papaver somniferum L. capsule wall specific subtractive cDNA library

text: Plasmid

text: pGEM-T


text: Xi'an Jiaotong University

text: live

text: Generic

text: bladder transitional cell

characteristic: cell type

text: BLX

characteristic: cell-line

text: A differentially expressed gene subtractive library was constructed by using subtractive suppression hybridization between the pair of bladder transitional cell lines BLX and BLS-211 with different cell shape. These EST fragments were ligated with pGEM-T vectors and amplified in JM109 E. coli. After blue-white screening, the white positive inserts were picked out and stored.

text: adult

characteristic: dev-stage

text: bladder

text: Homo sapiens

text: male

text: bladder transitional cell carcinoma cell lines subtractive library BLX

text: Plasmid

text: pGEM-T


text: National Institute of Agrobiological Sciences

characteristic: INSDC last update

text: live

text: Generic

text: 4th-instar day-2 larva

text: Bombyx mori

text: mixed

text: F1 (J150 x J203)

characteristic: strain

text: midgut


text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: wandering stage

text: Bombyx mori

text: mixed

text: prothoracic gland

text: prW-


text: live

text: Generic

text: 100hr after fertilization

text: Bombyx mori

characteristic: organism

text: mixed

text: embryo


text: live

text: Generic

text: pupal stage day-8

text: Bombyx mori

text: female

text: follicle cells

characteristic: title


text: live

text: Generic

text: 4th-instar day-2 larva

text: Bombyx mori

text: mixed

text: F1 (J150 x J203)

text: midgut

text: FJsb


text: live

text: Generic

text: 4th-instar day-3 larva

text: Bombyx mori

text: mixed

text: midgut

text: JFsb


text: live

text: Generic

text: 5th-instar day-3 larva

text: Bombyx mori

text: female

text: fat body

text: fbf


text: live

text: Generic

text: 5th-instar day-3 larva

text: Bombyx mori

text: male

text: fat body

text: fbm


text: live

text: Generic

text: spinning stage day-0

text: Bombyx mori

text: female

text: ovary


text: live

text: Generic

text: adult moth

text: Bombyx mori

text: Shuko x Ryuhaku

text: antenna

text: an--


text: live

text: Generic

text: 4th molting stage, day 2

text: Bombyx mori

text: male

text: verson's gland


text: live

text: Generic

text: P0 (just after pupation)

text: Bombyx mori

text: mixed

text: brain

text: br--


text: live

text: Generic

text: 5th-instar day-3 larva

text: Bombyx mori

text: mixed

text: midgut

text: mg


text: live

text: Generic

text: BmN

text: Bombyx mori

text: ovary

text: n


text: live

text: Generic

text: BmN

text: Bombyx mori

text: ovary

text: Nnor


text: live

text: Generic

text: BmN

text: BmNPV infected; 6 hr postinfection

text: Bombyx mori

text: ovary


text: live

text: Generic

text: BmN

text: BmNPV infected; 12 hr postinfection

text: Bombyx mori

text: ovary


text: live

text: Generic

text: 40 hr after oviposition

text: Bombyx mori

text: mixed

text: diapause-destined embryo


text: live

text: Generic

text: 96 hr after HCl treatment (HCl at 20 hr after oviposition)

text: Bombyx mori

text: mixed

text: diapause-cancelled embryo


text: live

text: Generic

text: spinning stage day-0

text: Bombyx mori

text: mixed

text: hemocyte


text: live

text: Generic

text: 5th-instar day-4 larva

text: Bombyx mori

text: mixed

text: prothoracic gland

text: prgv


text: live

text: Generic

text: 5th-instar day-3 larva

text: Bombyx mori

text: mixed

text: wing disc


text: live

text: Generic

text: spinning stage day-0

text: Bombyx mori

text: mixed

text: wing disc


text: live

text: Generic

text: spinning stage day-2

text: Bombyx mori

text: mixed

text: wing disc


text: live

text: Generic

text: 5th-instar day-4 larva

text: Bombyx mori

text: mixed

text: wing disc


text: Zhejiang University

text: live

text: Generic

text: Epinephelus awoara

text: Spleen

text: Epinephelus awoara suppression substractive hybridization library


text: University of Hyderabad ; International Crops Research Institute for the Semi-Arid Tropics (ICRISAT)

text: live

text: Generic

text: ICMH02185 (843A x ICMR 01029)

characteristic: cultivar

text: GCP-1G is a differential cDNA library constructed in order to capture the genes which are differentially expressed in a drought tolerant hybrid ICMH02185. A drought succeptible hybrid HHB67(843A X H77/833-2) was used as driver which was also subjected to drought stress along with the tester(ICMH02185).

characteristic: description

text: 48 days

text: Cenchrus americanus

text: EcoRI

text: XhoI

text: Leaf

text: pBlueScript SK(-)

characteristic: vector


text: live

characteristic: INSDC status

text: Generic

text: HHB67 (843A x H77/833-2)

text: GCP-3G is a differential cDNA library constructed in order to capture the genes which are differentially expressed in a drought sensitive hybrid HHB67 under drought stress. Unstressed HHB67 was used as driver.

text: 48 days

text: Cenchrus americanus

text: EcoRI

text: XhoI

text: Leaf

characteristic: tissue-type

text: pBlueScript SK(-)


characteristic: External Id

text: McGill University Macdonald Campus

characteristic: INSDC center name

text: live

text: Generic

text: SSH cDNA library was prepared from RNA of Rhizoctonia solani and Stachybotrys elegans growing in interaction as tester, and RNA of Rhizoctonia solani and Stachybotrys elegans growing alone as driver. The RNA was a pool of different time periods (about 12h before contact, day of contact, 2 days, 4 days, 6 days, 8 days, 10 days, and 12 days after contact of both hyphae). The cDNA isolated with SSH were cloned in the vector PcR2.4. The inserts were amplified by PCR and the corresponding amplicons were printed on microarray slides. The up-regulated genes were identified by microarray.

text: Rhizoctonia solani

text: ATCC 10183

text: Mycelium

text: Rhizoctonia solani SSH up-regulated cDNA library during mycoparasitism

text: PcR2.4 (Invitrogen)


text: University of Tokyo

characteristic: INSDC first public

text: live

text: Generic

text: Fertility related EST

text: Silene latifolia

text: male

characteristic: sex

text: young flower bud

text: Silene latifolia young flower bud


text: Oklahoma State University

text: live

text: Generic

text: adult

text: DH5 alpha

characteristic: lab-host

text: Sus scrofa

text: SalI

text: NotI

text: Male and Female

text: adipose tissue

text: porcine adipose tissue cDNA library (PADT)


text: Okazaki National Research Institutes

text: live

text: Generic

text: Fly gonads were isolated from embryos carrying EGFP-vasa transgene that express GFP only in poll cells by using fluorescence-activated cell sorting (FACS). Total RNA was extracted from the isolated gonads at embryonic stages 13-16 by using RNeasy Mini kit (QIAGEN). Poly(A)+ RNA was purified Oligotex dT30 (TaKaRa) from the total RNA. cDNA was synthesized and amplified by Long-Distance PCR using SMART technology (Clontech) and directionally cloned into pDNR-LIB vector (Clontech) cDNA inserts are flanked by linker sequences 5'-GGCCATTACGGCCGGG-3' (5' linker: SMART IV Oligonucleotide, Clontech) and 5'-polyA-CATGTCGGCCGCCTCGGCC-3' (3' linker: CDSIII/3' PCR Primer, Clontech).

text: 10-18 hours embryo (stage 13-16)

characteristic: dev-stage

text: Drosophila melanogaster

text: mixture of male and female

text: vasa-EGFP

text: gonad

text: Drosophila melanogaster embryonic gonads cDNA (pDNR-LIB)

text: pDNR-LIB


text: Advanced Technologies (Cambridge) Ltd

characteristic: INSDC last update

text: live

text: Generic

text: Before topping

text: Nicotiana tabacum

text: Flower


text: Altadis - Institut du Tabac

text: live

text: Generic

text: Before topping

text: Nicotiana tabacum

text: Root


text: J. Craig Venter Institute

text: live

text: Generic

text: Drosophila willistoni


text: Kazusa DNA Research Institute

text: live

text: Generic

text: isolate=Gifu B-129; Roots of hypernodulating mutant Ljsym16, rich in nodule primordia

text: Lotus japonicus

text: EcoRI

text: XhoI

text: nodulating roots

text: Lotus japonicus Gifu B-129 library

text: pBluescriptII SK-


text: Kazusa DNA Research Institute

text: live

text: Generic

text: roots of hypernodulating mutant Ljsym16, rich in nodule primordia

text: Lotus japonicus

text: ecotype Gifu B-129

characteristic: strain

text: nodulating root

text: Lotus japonicus nodulating root


text: Kazusa DNA Research Institute

text: live

text: Generic

characteristic: NCBI submission model

text: Lotus japonicus

text: ecotype Miyakojima MG-20

text: roots

text: Lotus japonicus roots


text: Kazusa DNA Research Institute

text: live

text: Generic

characteristic: NCBI submission package

text: Lotus japonicus

text: ecotype Miyakojima MG-20

text: flower

text: Lotus corniculatus var. japonicus flower


text: Kazusa DNA Research Institute

text: live

text: Generic

text: Lotus japonicus

characteristic: organism

text: ecotype Miyakojima MG-20

text: flower bud

text: Lotus corniculatus var. japonicus flower bud


text: Kazusa DNA Research Institute

text: live

text: Generic

text: pod (less than 20 mm in length)

text: Lotus japonicus

text: ecotype Miyakojima MG-20

text: pods

text: Lotus corniculatus var. japonicus pods (less than 20 mm in length)

characteristic: title


text: Kazusa DNA Research Institute

text: live

text: Generic

text: root of hypernodulating mutant har1-3, rich in nodule primordia

text: Lotus japonicus

text: ecotype Gifu B-129

text: nodulating root

text: Lotus corniculatus var. japonicus nodulating root


text: Ludwig-Maximilians-Universitat (LMU)

text: live

text: Generic

text: B-129 Gifu

characteristic: cultivar

text: 50 ug of total RNA prepared from L. japonicus roots were used for PolyA+ extraction on Dyna beads (Dynal, Norway) according to the manufacturer's instructions, which was subjected to cDNA-synthesis. The cDNA-AFLP analysis was performed as described (Durrant, W.E., Rowland, O., Piedras, P., Hammond-Kosack, K.E., and Jones, J.D. (2000). cDNA-AFLP reveals a striking overlap in race-specific resistance and wound response gene expression profiles. Plant Cell 12, 963-977). The [33P]-labelled cDNA-fragments were separated on 6% PAA-gels and visualized by autoradiography. Differential cDNA-AFLP fragments were cut out of the gel and reamplified. DNA sequences of reamplified cDNA-AFLP fragments were determined by either direct sequencing or after cloning into pGEMT-EASY.

text: root

characteristic: organ

text: Lotus japonicus

text: cDNA-AFLP fragments from Lotus japonicus roots two weeks after inoculation with Glomus intraradices


text: Ludwig-Maximilians-Universitat (LMU)

text: live

text: Generic

text: B-129 Gifu; mutant allele castor-2

characteristic: description

text: root

text: Lotus japonicus

text: cDNA-AFLP fragments from Lotus japonicus castor-2 mutant roots two weeks after inoculation with Glomus intraradices


text: Ludwig-Maximilians-Universitat (LMU)

text: live

characteristic: INSDC status

text: Generic

text: B-129 Gifu; mutant allele sym15-2

text: root

text: Lotus japonicus

text: cDNA-AFLP fragments from Lotus japonicus sym15-2 mutant roots two weeks after inoculation with Glomus intraradices


text: Ludwig-Maximilians-Universitat (LMU)

text: live

text: Generic

text: B-129 Gifu

text: root

text: Lotus japonicus

text: cDNA-AFLP fragments from Lotus japonicus roots two weeks after inoculation with Mesorhizobium loti


text: Ludwig-Maximilians-Universitat (LMU)

text: live

text: Generic

text: B-129 Gifu

text: root

text: Lotus japonicus

text: cDNA-AFLP fragments from Lotus japonicus roots


text: Ludwig-Maximilians-Universitat (LMU)

text: live

text: Generic

text: B-129 Gifu

text: 2 ug of total RNA prepared from L. japonicus roots were used for Oligo-dT-primed Reverse Transcription and subsequent PCR as described (Stracke S, Kistner C, Yoshida S, Mulder L, Sato S, Kaneko T, Tabata S, Sandal N, Stougaard J, Szczyglowski K and Parniske M (2002) A plant receptor-like kinase required for both bacterial and fungal symbiosis. Nature 417:959-962)

text: root

text: Lotus japonicus

text: cDNA fragments from Lotus japonicus roots two weeks after inoculation with Glomus intraradices


text: Ludwig-Maximilians-Universitat (LMU)

text: live

text: Generic

text: B-129 Gifu

text: root

text: Lotus japonicus

text: cDNA fragments from Lotus japonicus castor-2 mutant roots two weeks after inoculation with Glomus intraradices


text: live

text: Generic

text: B-129 Gifu

text: root

text: Lotus japonicus

text: cDNA fragments from Lotus japonicus roots two weeks after inoculation with Mesorhizobium loti


text: 1. Central Salt and Marine Chemicals Research Institute; 2. International Centre for Genetic Engineering and Biotechnology

text: live

text: Generic

text: 45 days

text: Salicornia brachiata

text: XhoI

text: Entire succulent tissue

characteristic: tissue-type

text: Salicornia brachiata succulent tissue

text: Phage

text: Lambda Zap

characteristic: vector


characteristic: External Id

text: J. Craig Venter Institute

characteristic: INSDC center name

text: live

text: Generic

text: Drosophila willistoni


text: Israel Oceanographic and Limnological Research

characteristic: INSDC first public

text: live

text: Generic

text: The library was prepared according to the subtractive- suppression hybridization method (SSH;Diatchenko, L., Lukyanov, S., Lau, Y.F.C. Siebert, P.D. 1999. Suppression subtractive hybridization: A versatile method for identifying differentially expressed genes. In: cDNA Preparation and Characterization, Meth. Enzymol. 303: 349-380) using two commercial kits (Clontech): SMART and PCR-select. cDNAs were cloned into P-GEM-T easy plasmid vectors transformed into the E. coli strain JM109. The hepatic cDNAs were cloned from fish exposed to a battery of pollutants.

text: Liver

text: Lithognathus mormyrus

text: Striped sea bream hepatic library

text: Plasmid

text: p-GEM-T easy


text: Agriculture and Agri-food Canada

text: live

text: Generic

text: Roblin

text: Wheat plants (cv Roblin) were grown under a 16 h light (20oC) /8 h dark (16oC) regime until mid-anthesis. Heads at mid-anthesis were point-inoculated with Fg spores (10 µl of 1 X 105 spores/ml) at every fully developed spikelet. Water inoculation was used as an experimental control on separate plants. Inoculated plants were misted for 10- second intervals every 15 min for 2 days. Inoculated spikelets were harvested 4 days post inoculation. Total RNA was extracted using guanidinium thiocyanate followed by pelleting through CsCl2. Poly(A+)RNA was isolated following the manufacturer's instructions using MPG Streptavidin Biotinylated Oligo (dT)25 Complex (CPG, NJ). A subtraction suppression hybridization (SSH) library was generated with the PCR-Select cDNA Subtraction Kit (BD Biosciences, Missisauga, ON, Canada), using cDNA from F. graminearum-inoculated tissues as the tester, subtracted with water-inoculated cDNA as the driver. The enriched SSH PCR products were cloned into the pGEM T-easy vector (Promega).

text: 4 days after anthesis

text: E. coli Sure Cells

characteristic: lab-host

text: Triticum aestivum

text: EcoRI

text: EcoRI

text: spikelets

text: Ta07_AAFC_ECORC_Fusarium_graminearum_inculated_wheat_heads

text: Plasmid

text: pGem-T easy


text: Agriculture and Agri-food Canada

text: live

text: Generic

text: Roblin

text: 4 days after anthesis

characteristic: dev-stage

text: E. coli Sure Cells

text: Triticum aestivum

text: EcoRI

text: EcoRI

text: spikelets

text: Ta07b_AAFC_ECORC_Fusarium_graminearum_inculated_wheat_heads

text: Plasmid

text: pGem-T easy


text: Agriculture and Agri-food Canada

characteristic: INSDC last update

text: live

text: Generic

text: Roblin

text: Wheat plants (cv Roblin) were grown under a 16 h light (20oC) /8 h dark (16oC) regime until mid-anthesis. Heads at mid-anthesis were point-inoculated with Fg spores (10 ul of 1 X 105 spores/ml) at every fully developed spikelet. Water inoculation was used as an experimental control on separate plants. Inoculated plants were misted for 10- second intervals every 15 min for 2 days. Inoculated spikelets were harvested 2 days post inoculation. Total RNA was extracted using guanidinium thiocyanate followed by pelleting through CsCl2. Poly(A+)RNA was isolated following the manufacturer's instructions using MPG Streptavidin Biotinylated Oligo (dT)25 Complex (CPG, NJ). A subtraction suppression hybridization (SSH) library was generated with the PCR-Select cDNA Subtraction Kit (BD Biosciences, Missisauga, ON, Canada), using cDNA from F. graminearum-inoculated tissues as the tester, subtracted with water-inoculated cDNA as the driver. The enriched SSH PCR products were cloned into the pGEM T-easy vector (Promega).

text: 2 days after anthesis

text: E. coli Sure Cells

text: Triticum aestivum

text: EcoRI

text: EcoRI

text: spikelets

text: Ta08_AAFC_ECORC_Fusarium_graminearum_inculated_wheat_heads

text: Plasmid

text: pGem-T easy


text: The First Institute of Oceanography

text: live

text: Generic

text: Haliotis discus hannai lno

text: Haliotis discus hannai

text: female

characteristic: sex

text: Haliotis discus Hannailno Liver and Kidney cDNA library


text: Forestry and Forest Products Research Institute

text: live

text: Generic

characteristic: NCBI submission model

text: The library was constructed using ZAP-cDNA method by Stratagene. Clones to be sequenced were prepared by mass excision. The suffix 'F' indicates that M13 Reverse primer was used for sequencing. The suffix 'FR' indicates that M13 reverse primer was used for sequencing and run of 10 or more T residues was presented at the beginning of the sequence. The suffix 'R' indicates that T7 primer was used for sequencing. The suffix 'RF' indicates that T7 primer was used for sequencing and run of 10 or more A residues was presented at the end of cDNA insert. The suffix 'RR' indicates that T7 primer was used for sequencing and run of 10 or more T residues was presented at the beginning of the sequence. The suffix 'RR' indicates that T7 primer was used for sequencing and run of 10 or more T residues was presented at the beginning of the sequence. The suffix 'TR' indicates that dT anchored primer was used for sequencing.

text: Cryptomeria japonica

text: EcoRI

text: XhoI

text: pollen

text: Cryptomeria japonica pollen

text: Lambda Uni-ZAP XR, excised phagemid pBluescript SK(-)


text: Kyoto Prefectural University

text: live

text: Generic

characteristic: NCBI submission package

text: Triticum aestivum

text: shoot grown with desiccation

text: Y.Ogihara unpublished cDNA library Wh_SHDr


text: Kyoto Prefectural University

text: live

text: Generic

text: Valuevskaya

text: Triticum aestivum

characteristic: organism

text: shoot grown under cold condition for 16days

text: Y.Ogihara unpublished cDNA library Wh_V4816


text: Kyoto Prefectural University

text: live

text: Generic

text: Valuevskaya

text: Triticum aestivum

text: shoot grown under cold condition for 3days

text: Y.Ogihara unpublished cDNA library Wh_V483

characteristic: title


text: Kyoto Prefectural University

text: live

text: Generic

text: Valuevskaya

characteristic: cultivar

text: Triticum aestivum

text: shoot with ABA treatment

text: Y.Ogihara unpublished cDNA library Wh_VABA


text: Kyoto Prefectural University

text: live

text: Generic

text: Valuevskaya

text: Triticum aestivum

text: shoot grown with desiccation

text: Y.Ogihara unpublished cDNA library Wh_VWD


text: Kyoto Prefectural University

text: live

text: Generic

text: Valuevskaya

text: Triticum aestivum

text: shoot with heat shock treatment

text: Y.Ogihara unpublished cDNA library Wh_VHS


text: Kyoto Prefectural University

text: live

text: Generic

text: Valuevskaya

text: Triticum aestivum

text: liquid cultured tissue

text: Y.Ogihara unpublished cDNA library Wh_VSCB


text: Kyoto Prefectural University

text: live

text: Generic

text: Chinese Spring

text: Triticum aestivum

text: anther at meiosis

text: Y.Ogihara unpublished cDNA library Wh_PCDAM


text: Kyoto Prefectural University

text: live

text: Generic

text: Chinese Spring

text: Triticum aestivum

text: seeding

text: Y.Ogihara unpublished cDNA library Wh_SH


text: Kyoto Prefectural University

text: live

text: Generic

text: Chinese Spring

text: early flowering

text: Triticum aestivum

text: spikelet

text: Y.Ogihara unpublished cDNA library Wh_FL


text: Kyoto Prefectural University

text: live

text: Generic

text: Chinese Spring

text: Triticum aestivum

text: seed DPA20

text: Y.Ogihara unpublished cDNA library Wh_DPA20


text: Kyoto Prefectural University

text: live

text: Generic

text: Triticum aestivum

text: dormant seed with cold treatment after water absorption

text: Y.Ogihara unpublished cDNA library Wh_EMC


text: Kyoto Prefectural University

text: live

text: Generic

text: Triticum aestivum

text: dormant seed with water absorption after breaking

text: Y.Ogihara unpublished cDNA library Wh_EMI


text: Kyoto Prefectural University

text: live

text: Generic

text: Triticum aestivum

text: dormant seed with water absorption

text: Y.Ogihara unpublished cDNA library Wh_EM


text: Kyoto Prefectural University

text: live

text: Generic

text: Triticum aestivum

text: anther at meiosis

text: Y.Ogihara unpublished cDNA library Wh_GCPCDAM


text: Kyoto Prefectural University

text: live

text: Generic

text: Triticum aestivum

text: anther at meiosis

text: Y.Ogihara unpublished cDNA library Wh_HGCPCDAM


text: Kyoto Prefectural University

text: live

text: Generic

text: Kmppd(CS)

text: Triticum aestivum

text: shoot grown under continuous light

text: Y.Ogihara unpublished cDNA library Wh_KMP


text: Kyoto Prefectural University

text: live

text: Generic

text: Chinese Spring

text: Triticum aestivum

text: shoot grown with continuous light after cold treatment

text: Y.Ogihara unpublished cDNA library Wh_KMV


text: Kyoto Prefectural University

text: live

text: Generic

text: Triticum aestivum

text: seed DPA5

text: Y.Ogihara unpublished cDNA library Wh_MS


text: Kyoto Prefectural University

text: live

text: Generic

text: Chinese Spring

text: Triticum aestivum

text: seed DPA5

text: Y.Ogihara unpublished cDNA library Wh_OKCS


text: Kyoto Prefectural University

text: live

text: Generic

text: Triticum aestivum

text: root of desiccated seed

text: Y.Ogihara unpublished cDNA library Wh_RDr


text: Kyoto Prefectural University

text: live

text: Generic

text: Triticum aestivum

text: shoot grown with cold treatment

text: Y.Ogihara unpublished cDNA library Wh_SHC


text: Kyoto Prefectural University

text: live

text: Generic

text: Chinese Spring

text: Triticum aestivum

text: callus

text: Y.Ogihara unpublished cDNA library Wh_CSEC


text: Institute of Life Sciences

text: live

text: Generic

text: China native BmNPV-resistante strain

text: 5th-instar day-2 larva

text: midgut

characteristic: organ

text: Bombyx mori

text: mixed

characteristic: strain

text: Bombyx mori midgut cDNA Library

text: Plasmid


text: Department of Radiation Medicine, Second Military Medical University

text: live

characteristic: INSDC status

text: Generic

text: tracheal epithelium

characteristic: cell type

characteristic: cell-line

text: Homo sapiens

text: Homo sapiens tracheal epithelium cell line BEAS-2B


characteristic: External Id

text: Max F. Perutz Laboratories, University Departments at the Vienna Biocenter

text: live

text: Generic

text: stress-induced, embryogenic microspores

text: Petit Havana SR1

text: Nicotiana tabacum

text: EcoRI

text: XhoI

text: embryogenic microspores

text: pBluescript II KS (-)

characteristic: vector


text: Melaka Institute of Biotechnology

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: Somatic

text: oleifera (Malaysian)

text: The cDNA library was prepared following all the instructions and the protocols given by manufacturer (Stratagene) of the cDNA synthesis kit.

characteristic: description

text: 15 week after anthesis (WAA)

text: Fruit

text: Elaeis oleifera

text: EcoRI

text: XhoI

text: Mesocarp

characteristic: tissue-type

text: Oil palm Lambda ZAP-II Express Library

text: Phage

text: Lambda ZAP-II


text: National Cancer Institute / NIH

characteristic: INSDC last update

text: live

text: Generic

text: cDNA was primed using oligo-dT primer: GACTAGTTCTAGATCGCGAGCGGCCGCC(T)25 and cloned into the SmaI/NotI sites of pCS111. Size-selection >1.25kb resulted in an average insert size of 1.9 kb. This primary library was constructed by Express Genomics (Frederick, MD). Note: this is a (http://xgc.nci.nih.gov/) Xenopus Gene Collection library.

text: DH10B TonA

characteristic: lab-host

text: thymus

text: Xenopus laevis

text: SmaI

text: NotI

text: thymus/thymocytes

text: plasmid


text: State key Laboratory of Tropical crop Biotechnology Chinese Academy of Tropical Agriculture Sciences

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Hevea brasiliensis

text: Hevea brasiliensis latex SSH


text: University of Southern California

text: live

text: Generic

text: Normalized cDNA library prepared from hypoxia challenged animals

text: Adult

characteristic: dev-stage

text: E.coli Electromax DH10B

text: Gillichthys mirabilis

characteristic: organism

text: Sfi1 GGCCATTACGGCC

text: Sfi1 GGCCGCCTCGGCC

text: Male & female

characteristic: sex

text: Liver and muscle

text: Gillichthys liver & muscle library 1

characteristic: title

text: Plasmid


text: University of Southern California

text: live

characteristic: INSDC status

text: Generic

text: Normalized and serially subtracted cDNA library prepared from envrionmentally challenged animals

text: Adult

text: E.coli Electromax DH10B

text: Gillichthys mirabilis

text: Sfi1 GGCCATTACGGCC

text: Sfi1 GGCCGCCTCGGCC

text: Male & female

text: Liver, muscle, brain, heart, intestine

text: Gillichthys mixed tissue (liver, muscle, brain, heart, intestine) library 1

text: Plasmid


text: University of Southern California

text: live

text: Generic

text: Normalized and serially subtracted cDNA library prepared from liver of muscle of environmentally challenged animals

text: Adult

text: E.coli Electromax DH10B

text: Gillichthys mirabilis

text: Sfi1 GGCCATTACGGCC

text: Sfi1 GGCCGCCTCGGCC

text: Male & female

text: Liver

text: Gillichthys liver & muscle library 2

text: Plasmid


text: live

text: Generic

text: Normalized cDNA library prepared from heart tissue of hypoxia challenged animals

text: Adult

text: E.coli Electromax DH10B

text: Gillichthys mirabilis

text: Sfi1 GGCCATTACGGCC

text: Sfi1 GGCCGCCTCGGCC

text: Male & female

text: Heart muscle

text: Gillichthys heart library 1

text: Plasmid


text: University of Southern California

text: live

text: Generic

text: Normalized and serially subtracted cDNA library prepared from brain and eye tissues of environmentally challenged animals

text: Adult

text: E.coli Electromax DH10B

text: Gillichthys mirabilis

text: Sfi1 GGCCATTACGGCC

text: Sfi1 GGCCGCCTCGGCC

text: Male & female

text: Brain & eye

text: Gillichthys brain & eye library 1

text: Plasmid


text: live

text: Generic

text: Normalized and serially subtracted cDNA library prepared from liver tissues of environmentally challenged animals

text: Adult

text: E.coli Electromax DH10B

text: Gillichthys mirabilis

text: Sfi1 GGCCATTACGGCC

text: Sfi1 GGCCGCCTCGGCC

text: Male & female

text: Liver

text: Gillichthys liver library 1

text: Plasmid


text: University of Southern California

text: live

text: Generic

text: Normalized and serially subtracted cDNA library prepared from brain and eye tissue of environmentally challenged animals

text: Adult

text: E.coli Electromax DH10B

text: Gillichthys mirabilis

text: Sfi1 GGCCATTACGGCC

text: Sfi1 GGCCGCCTCGGCC

text: Male & female

text: Brain & eye

text: Gillichthys brain & eye library 2

text: Plasmid


text: live

text: Generic

text: Adult

text: E.coli Electromax DH10B

text: Gillichthys mirabilis

text: Sfi1 GGCCATTACGGCC

text: Sfi1 GGCCGCCTCGGCC

text: Male & female

text: Liver

text: Gillichthys brain & eye library 3

text: Plasmid


text: live

text: Generic

text: Serially subtracted cDNA library prepared from gill tissue of environmentally challenged animals

text: Adult

text: E.coli Electromax DH10B

text: Gillichthys mirabilis

text: Sfi1 GGCCATTACGGCC

text: Sfi1 GGCCGCCTCGGCC

text: Male & female

text: Muscle

text: Gillichthys gill library 1

text: Plasmid


text: University of Southern California

text: live

text: Generic

text: Serially subtracted cDNA library prepared from muscle of hypoxia challenged animals

text: Adult

text: E.coli Electromax DH10B

text: Gillichthys mirabilis

text: Sfi1 GGCCATTACGGCC

text: Sfi1 GGCCGCCTCGGCC

text: Male & female

text: Muscle

text: Gillichthys muscle library 1

text: Plasmid


text: University of Southern California

text: live

text: Generic

text: Serially subtracted cDNA library prepared from liver of hypoxia challenged animals

text: Adult

text: E.coli Electromax DH10B

text: Gillichthys mirabilis

text: Sfi1 GGCCATTACGGCC

text: Sfi1 GGCCGCCTCGGCC

text: Male & female

text: Liver

text: Gillichthys liver library 2

text: Plasmid


text: University of Southern California

text: live

text: Generic

text: cDNA library prepared from gill tissue of hyperosmotic, hypoxia and heat shocked animals

text: Adult

text: E.coli Electromax DH10B

text: Gillichthys mirabilis

text: Sfi1 GGCCATTACGGCC

text: Sfi1 GGCCGCCTCGGCC

text: Male & female

text: Muscle

text: Gillichthys gill library 2

text: Plasmid


text: University of Southern California

text: live

text: Generic

text: cDNA library prepared from suppression subtractive hybridization fragments enriched for environmentally regulated genes which were then biotinylated and used to capture full-length cDNAs. The captured cDNAs were cloned into the vector.

text: Adult

text: E.coli Electromax DH10B

text: Gillichthys mirabilis

text: Sfi1 GGCCATTACGGCC

text: Sfi1 GGCCGCCTCGGCC

text: Male & female

text: Liver, muscle, brain, heart, intestine

text: Captured - unused

text: Plasmid


text: University of Southern California

text: live

text: Generic

text: Normalized and serially subtracted cDNA library prepared from environmentally challenged animals

text: Adult

text: E.coli Electromax DH10B

text: Gillichthys mirabilis

text: Sfi1 GGCCATTACGGCC

text: Sfi1 GGCCGCCTCGGCC

text: Male & female

text: Liver, muscle, brain, heart, intestine

text: Gillichthys mixed tissue (liver, muscle, brain, heart, intestine) library 2

text: Plasmid


text: Vegetable and Flowers Institute

text: live

text: Generic

text: This is a differential expressed EST isolated from potato leaves infected with Ralstonia solanacearum using suppression subtractive hybridization.

text: Solanum phureja

characteristic: strain

text: leaf

text: Potato leaf cDNA library


text: Gene Research Center

text: live

text: Generic

text: Homo sapiens

text: Homo sapiens PCR product (Maegawa S)


text: Forestry and Forest Products Research Institute

text: live

text: Generic

text: Chamaecyparis obtusa

text: cambium and surrounding tissues

text: Chamaecyparis obtusa cambium and surrounding tissues


text: live

text: Generic

text: Cryptomeria japonica

text: cambium and surrounding tissues

text: Cryptomeria japonica cambium and surrounding tissues


text: live

text: Generic

text: Cryptomeria japonica

text: vegetative bud

text: Cryptomeria japonica vegetative bud


text: Michigan State University

text: live

text: Generic

characteristic: cultivar

text: cDNA synthesized from poplar developing cambium of stems treated by mechanical fexure and harvested 2, 4, 8, or 12 hours after final stress

text: 2, 4, 8, 12 hours after mechanical perturbation

text: Populus trichocarpa x Populus deltoides

text: Poplar developing cambium

text: phage

text: pBluescript


characteristic: External Id

text: School of Forest Resources and Conservation, University of Florida

text: live

text: Generic

text: Differential display. First strand cDNA synthesized by RT-PCR from mRNA expressed in poplar trees (approximately 60-100 cm tall at the start of the experiment) treated daily with 0 mM or 50 mM ammonium nitrate for 10 days.

text: Populus trichocarpa x Populus deltoides

text: shoot tips, mature leaves, and woody stems

text: Poplar limiting vs. luxuriant nitrogen

text: plasmid

text: pGEM-T


text: UMR INRA/Universite Henri Poincare 1136

text: live

text: Generic

text: 'Beaupre'

text: cDNA library of roots from two-month-old Populus trichocarpa Torr.& Gray x deltoides Bartr. Ex Marshall (clone 'Beaupre') grown for 19 days under restricted irrigation to reach 50% of the transpiration rate of fully watered plants. The cDNA library was constructed from 1 ug of total RNA using the SMART cDNA synthesis kit (Clontech, Palo Alto, CA, USA) according to the manufacturer's instructions. The resulting cDNA was packed into lambda phages using the Gigapack III Gold packaging kit (Stratagene, La Jolla, CA). The pTriplEx2 phagemid clones in Escherichia choli were obtained by using the mass in vivo excision protocol according to the manufacturer's instructions (Clontech).

text: two-month-old

text: root

characteristic: organ

text: Populus trichocarpa x Populus deltoides

text: two-month-old roots from clone 'Beaupre' grown for 19 days under restricted irrigation

characteristic: vector


text: UMR INRA/Universite Henri Poincare 1136

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: 'Beaupre'

text: cDNA library of two-month-old roots from Populus trichocarpa Torr.& Gray x deltoides Bartr. Ex Marshall (clone 'Beaupre'). The cDNA library was constructed from 1 ug of total RNA using the SMART cDNA synthesis kit (Clontech, Palo Alto, CA, USA) according to the manufacturer's instructions. The resulting cDNA was packed into lambda phages using the Gigapack III Gold packaging kit (Stratagene, La Jolla, CA). The pTriplEx2 phagemid clones in Escherichia choli were obtained by using the mass in vivo excision protocol according to the manufacturer's instructions (Clontech).

text: two-month-old

text: root

text: Populus trichocarpa x Populus deltoides

text: two-month-old roots from clone 'Beaupre'


text: University of Victoria

text: live

text: Generic

text: This EST originates from a systemically wound-induced hybrid poplar leaf cDNA library. The cDNAs were sequenced from the 5'-end using the T3 primer.

characteristic: description

text: Populus trichocarpa x Populus deltoides

text: Leaf

characteristic: tissue-type

text: hybrid poplar systemically wound-induced leaf cDNA library

text: Phagemid

text: pBluescript SK+


text: University of British Columbia

characteristic: INSDC last update

text: live

text: Generic

text: Cultured cells [de Sa M.M. et al. (1992) Plant Physiology 98:728-737] were grown in media (45mL) supplemented with either 50uM salicylic acid, 50uM benzothiadiazole, 50uM methyl jasmonate, 20ug chitosan or 200uL of Pollacia radiosa extract. Cells were harvested after a 3 hour treatment, along with untreated control cells. mRNA was isolated from each tissue source independently and equal quantities of mRNA from each tissue were then pooled. cDNA was prepared from 5 micrograms of mRNA and directionally ligated into the pBluescript II SK (+) XR vector using the pBluescript II XR cDNA Library Construction Kit according to manufacturer's instructions with modifications (Stratagene). Plasmid DNA was then transformed by electroporation into DH10B cells (Invitrogen) for propagation.

text: E. coli DH10B T1 phage resistant cells

characteristic: lab-host

text: Populus trichocarpa x Populus deltoides

text: EcoRI (5' end of cDNA)

text: XhoI (3' end of cDNA)

text: Male

text: pBluescript II SK (+) XR


text: University of British Columbia

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Cultured cells [de Sa MM et al. (1992) Plant Physiology 98:728-737] were grown in media (45mL) supplemented with either 50uM salicylic acid, 50uM benzothiadiazole, 50uM methyl jasmonate, 20ug chitosan or 200uL of Pollacia radiosa extract. Cells were harvested after a 3 hour treatment, along with untreated control cells. mRNA was isolated from each tissue source independently and equal quantities of mRNA from each tissue were then pooled. cDNA was prepared from 5 micrograms of mRNA and directionally ligated into the pBluescript II SK (+) XR vector using the pBluescript II XR cDNA Library Construction Kit according to manufacturer's instructions with modifications (Stratagene). Plasmid DNA was then transformed by electroporation into DH10B cells (Invitrogen) for propagation. Normalization was applied according to published methods [Bonaldo M.F. et al. (1996) Genome Research 6(9):791] in order to reduce the abundance of highly expressed transcripts.

text: E. coli DH10B T1 phage resistant cells

text: Populus trichocarpa x Populus deltoides

text: EcoRI (5' end of cDNA)

text: XhoI (3' end of cDNA)

text: Male

text: pBluescript II SK (+) XR


text: University of British Columbia

text: live

text: Generic

text: Sapling trees two metres in height and grown under greenhouse conditions were exposed to continuous feeding by Malacosoma disstria Hubner (forest tent caterpillar) mid-instar larvae caged on the sapling using mesh bags. Mature leaves from within the caged region were collected 2 hours, 12 hours, 24 hours and 48 hours after the onset of treatment. mRNA was isolated from each tissue source independently and equal quantities of mRNA from each tissue were then pooled. cDNA was prepared from 5 micrograms of mRNA and directionally ligated into the pBluescript II SK (+) XR vector using the pBluescript II XR cDNA Library Construction Kit according to manufacturer's instructions with modifications (Stratagene). Plasmid DNA was then transformed by electroporation into DH10B cells (Invitrogen) for propagation.

text: E. coli DH10B T1 phage resistant cells

text: Populus trichocarpa x Populus deltoides

characteristic: organism

text: EcoRI (5' end of cDNA)

text: XhoI (3' end of cDNA)

text: Male

text: pBluescript II SK (+) XR


text: University of British Columbia

text: live

text: Generic

text: Sapling trees one metre in height and grown under greenhouse conditions were exposed to continuous feeding by Malacosoma disstria Hubner (forest tent caterpillar) mid-instar larvae caged on the sapling using mesh bags. Mature leaves from within the caged region were collected 4 hours, 8 hours and 24 hours after the onset of treatment. Mature leaves were also collected above the caged region (systemic response) 4 hours, 12 hours and 48 hours after the onset of treatment. mRNA was isolated from each tissue source independently and equal quantities of mRNA from each tissue were then pooled. cDNA was prepared from 20 micrograms of mRNA according to the full-length cDNA library construction method described by Carninci P. et al. (2000), Genome Research 10(10):1617-1630 and directionally ligated into the pBluescript II SK (+) XR vector digested with SstI (5' end) and XhoI (3'). Plasmid DNA was then transformed by electroporation into DH10B cells (Invitrogen) for propagation.

text: E. coli DH10B T1 phage resistant cells

text: Populus trichocarpa x Populus deltoides

text: SstI (5' end of cDNA)

text: XhoI (3' end of cDNA)

text: Male

characteristic: sex

text: pBluescript II SK (+) XR


text: University of British Columbia

text: live

text: Generic

text: Sapling trees two metres in height and grown under greenhouse conditions were exposed to continuous feeding by Malacosoma disstria Hubner (forest tent caterpillar) mid-instar larvae caged on the sapling using mesh bags. Mature leaves from within the caged region were collected 2 hours, 12 hours, 24 hours and 48 hours after the onset of treatment. mRNA was isolated from each tissue source independently and equal quantities of mRNA from each tissue were then pooled. cDNA was prepared from 5 micrograms of mRNA and directionally ligated into the pBluescript II SK (+) XR vector using the pBluescript II XR cDNA Library Construction Kit according to manufacturer's instructions with modifications (Stratagene). Plasmid DNA was then transformed by electroporation into DH10B cells (Invitrogen) for propagation. Normalization was applied according to published methods [Bonaldo M.F. et al. (1996) Genome Research 6(9):791] in order to reduce the abundance of highly expressed transcripts.

text: E. coli DH10B T1 phage resistant cells

text: Populus trichocarpa x Populus deltoides

text: EcoRI (5' end of cDNA)

text: XhoI (3' end of cDNA)

text: Male

characteristic: title

text: pBluescript II SK (+) XR


text: University of British Columbia

text: live

characteristic: INSDC status

text: Generic

text: Sapling trees were grown to 30cm to 50cm in height in aerated hydroponic growth media under growth chamber conditions. Roots were harvested from untreated control trees at 0 hours, as well as from trees grown in media without a nitrogen source for 24 hours and 48 hours. mRNA was isolated from each tissue source independently and equal quantities of mRNA from each tissue were then pooled. cDNA was prepared from 5 micrograms of mRNA and directionally ligated into the pBluescript II SK (+) XR vector using the pBluescript II XR cDNA Library Construction Kit according to manufacturer's instructions with modifications (Stratagene). Plasmid DNA was then transformed by electroporation into DH10B cells (Invitrogen) for propagation.

text: E. coli DH10B T1 phage resistant cells

text: Populus trichocarpa x Populus deltoides

text: EcoRI (5' end of cDNA)

text: XhoI (3' end of cDNA)

text: Male

text: pBluescript II SK (+) XR


text: Kazusa DNA Research Institute;

text: live

text: Generic

text: suspension-cultured cells

text: Lotus japonicus

text: ecotype Gifu B-129

characteristic: strain

text: Lotus japonicus suspension-cultured cells


text: Kazusa DNA Research Institute;

text: live

text: Generic

text: protoplasts prepared from suspension-cultured cells

text: Lotus japonicus

text: ecotype Gifu B-129

text: Lotus japonicus protoplasts from suspension-cultured cells


text: Kazusa DNA Research Institute;

text: live

text: Generic

text: suspension-cultured cells treated with A) 0.5 mM salicylic acid for 6 hr and B) 0.5 mM methyljasmonate for 24 hr. Equal amounts of total RNA from A and B were mixed and used for construction of the cDNA library.

text: Lotus japonicus

text: ecotype Gifu B-129

text: Lotus japonicus salicylic acid and methyljasmonate treated cells


text: Aquaculture Protein Centre/Norwegian School of Veterinary Science

text: live

text: Generic

text: Suppression subtractive hybridization

text: Intestine

text: Gadus morhua

text: Atlantic cod intestine SSH

text: plasmid


text: Genoscope - Centre National de Sequencage

text: live

text: Generic

text: Hatchling to 1-day-old juveniles

characteristic: dev-stage

text: Spadella cephaloptera

text: whole animal

text: Spadella cephaloptera whole animal Hatchling to 1-day-old juveniles

text: pTriplex


characteristic: External Id

text: National Institute of Agrobiological Sciences

text: live

text: Generic

text: Single sequence runs of clones from a forward-subtracted cDNA library of Enchytraeus japonensis regenerating fragments.

text: Enchytraeus japonensis

text: artificially-cut body fragments

text: Enchytraeus japonensis forward-subtracted cDNA artificially-cut body fragments


text: live

text: Generic

text: Single sequence runs of clones from a reverse-subtracted cDNA library of Enchytraeus japonensis. The subtraction was done between regenerating body fragments and intact worms

text: Enchytraeus japonensis

text: whole body

text: Enchytraeus japonensis reverse-subtracted cDNA whole body of intact worms


text: live

text: Generic

text: Single sequence runs of clones from a forward-subtracted cDNA library of Enchytraeus japonensis. The subtraction was done between regenerating body fragments and intact worms.

text: Enchytraeus japonensis

text: Regenerating fragments of E. japonensis

text: Enchytraeus japonensis forward-subtracted cDNA Regenerating fragments


text: NewBioTechnic, S.A. (NBT)

text: live

text: Generic

text: Trichoderma virens

text: NBT T59

text: mycelium Lambda Zap Library L20


text: NewBioTechnic, S.A. (NBT)

text: live

text: Generic

text: Trichoderma viride

text: NBT T78

text: mycelium Lambda Zap Library L21


text: Ruhr-Universitaet Bochum

text: live

text: Generic

text: Pyronema omphalodes

text: CBS 100304

text: Pyronema omphalodes CBS 100304


text: INRA, Genetique Animale, Genetique cellulaire

text: live

text: Generic

text: adult muscle

text: Sus scrofa

text: muscle

text: Normalised cDNA library, adult muscle (longissimus dorsi) (pmus)


text: Rowett Research Institute

text: live

text: Generic

text: Dasytricha ruminantium

text: Dasytricha ruminantium library (McEwan NR)


text: Rowett Research Institute

text: live

text: Generic

text: Diploplastron affine

text: Diploplastron affine library (McEwan NR)


text: Rowett Research Institute

text: live

text: Generic

text: Entodinium caudatum

text: Entodinium caudatum library (McEwan NR)


text: Rowett Research Institute

text: live

text: Generic

text: Epidinium ecaudatum

text: Epidinium ecaudatum library (McEwan NR)


text: Rowett Research Institute

text: live

text: Generic

text: Eudiplodinium maggii

text: Eudiplodinium maggii library (McEwan NR)


text: Rowett Research Institute

text: live

text: Generic

text: Isotricha prostoma

text: Isotricha prostoma library (McEwan NR)


text: Rowett Research Institute

text: live

text: Generic

text: Metadinium medium

text: Metadinium medium library (McEwan NR)


text: Rowett Research Institute

text: live

text: Generic

text: Polyplastron multivesiculatum

text: Polyplastron multivesiculatum library (McEwan NR)


text: Consorci CSIC-IRTA

text: live

text: Generic

text: Arabidopsis thaliana

text: ecotype Col0

text: seed

text: Arabidopsis thaliana Col0 seed


text: live

text: Generic

text: 5 weeks

text: Sus scrofa

text: male

text: cerebella

text: full-length enriched pig cDNA library, cerebella


text: Kobe University

text: live

text: Generic

text: heart

characteristic: organ

text: Coturnix japonica

text: female

text: Zap Express Japanese quail heart cDNA


text: Kobe University

text: live

text: Generic

text: 5 days embryo

text: Coturnix japonica

text: Zap Express Japanese quail embryo cDNA


text: Centro de Investigacion Cientifica de Yu

text: live

text: Generic

text: alto Atlantico

characteristic: cultivar

text: chitosan-elicited tissues

text: Cocos nucifera

text: calli

text: Cocos nucifera calli


text: University of Wisconsin-Milwaukee

characteristic: INSDC center name

text: live

text: Generic

text: Library created using PCR-Select cDNA Subtraction (Clonetech) using pooled liver and gonad of male and female adult fathead minnows and subtracting samples from fish exposed to dietary methylmercury (3.93 ( .08 ug g-1) versus controls. Subtractions were in both directions. The resultant pool of cDNA clones obtained from the PCR reaction were shotgun ligated into pGEM T-Easy cloning vector (Promega, Madison, WI) and then transformed into DH5a cells and plated onto Luria-Bertani (LB) agar plates containing ampicillin and oxacillin (100 ug/mL each).

text: adult

text: Pimephales promelas

text: male and female

text: liver

text: Fathead minnow methylmercury SSH cDNA Library


text: Faculty of Sciences of Sfax - Dept of Biology

characteristic: INSDC first public

text: live

text: Generic

text: Deglet Nour

text: Zygotic embryos were excised from date palm seed CV. Deglet Nour, RNAs were prepared and used in RT-PCR experiment amorces. One storage proteins isolated and sequenced from mature zygotic embryo. The correspondence cDNA fragment was synthesized using degenerated primers. Amplified fragment was isolated and cloned in pGEM-T Easy vector.

text: seed dormant embryo

text: Phoenix dactylifera

text: embryos

text: date palm zygotic embryos cDNA

text: Plasmid

text: pGEM-T Easy

characteristic: vector


text: National Institute of Agrobiological Sciences

text: live

text: Generic

text: 5th-instar day-3 larva

text: Bombyx mori

text: mixed

text: maxillary galea

characteristic: tissue-type

text: mxg-


characteristic: INSDC last update

text: live

text: Generic

text: 5th-instar day-3 larva

text: Bombyx mori

text: mixed

text: middle silkgland


text: live

text: Generic

text: 5th-instar day-3 larva

text: Bombyx mori

text: mixed

text: posterior silkgland

text: psgV


text: Institute for Animal Health

text: live

text: Generic

text: macrophage

characteristic: cell type

text: Sus scrofa

text: lung

text: foward subtractive cDNA library, stimulated minus unstimulated macrophages


text: Institute for Animal Health

text: live

text: Generic

characteristic: NCBI submission model

text: macrophage

text: Sus scrofa

text: lung

text: foward subtractive cDNA library, ASFV infected minus uninfected macrophages


text: Institute for Animal Health

text: live

text: Generic

characteristic: NCBI submission package

text: macrophage

text: Sus scrofa

text: lung

text: foward subtractive cDNA library, macrophages minus myocardia


text: Institute for Animal Health

text: live

text: Generic

text: macrophage

text: Sus scrofa

characteristic: organism

text: lung

text: subtractive cDNA library, uninfected minus ASFV infected macrophages


text: Institute for Animal Health

text: live

text: Generic

text: macrophage

text: Sus scrofa

text: lung

text: subtractive cDNA library, stimulated minus unstimulated macrophages


text: Institute for Animal Health

text: live

text: Generic

text: macrophage

text: Sus scrofa

text: lung

text: subtractive cDNA library, unstimulated minus stimulated macrophages


text: East Tennessee State University

text: live

text: Generic

text: Citrus x paradisi

text: Duncan variety

text: Citrus paradisi young grapefruit leaf cDNA library

text: pDONR 222 (Invitrogen)


text: Sogang University

text: live

text: Generic

text: Chuchung

text: reproductive stage

text: E. coli

characteristic: lab-host

text: flower

text: Oryza sativa

text: hermaphrodite

characteristic: sex

text: Rice flower differential display

text: plasmid

text: a modified pBluescript KS(-) vector that contained a single 3'-T-overhang


text: Iowa State University

text: live

characteristic: INSDC status

text: Generic

text: Fully expanded leaves of mature greenhouse grown Nicotiana langsdorffii X Nicotiana sanderae (LXS8) plants

characteristic: description

text: fully expanded leaves from mature plants

text: Leaf

text: Nicotiana langsdorffii x Nicotiana sanderae

text: Sfi 1A

text: Sfi 1B

text: Ornamental tobacco (LxS8) leaf cDNA library

characteristic: title

text: plasmid

text: pDNR-LIB