text: MPI for Molecular Genetics

text: live

text: Generic

text: Total RNA was extracted from 5 male specimens of weight ranging between 350g and 4kg. Collected tissue frozen in liq N2 and stored at -80C prior to RNA extraction. mRNA purification and concentration were performed using the Dynal's oligo-dT magnetic beads. Library construction was performed with Creator SMART cDNA library construction kit (Clontech) and the thermostable duplex-specific nuclease (Zhu et al., 2001). Initial LD PCR amplification of 22 cycles for enrichment of full length transcripts was verified on Agarose gels. cDNA sizes (>500 base pairs) were selected using filter columns based on agarose gels as described in the Clontech manual. Library construction and sequencing were performed at the MPI of Molecular Genetics Berlin. isolation_source: aquaculture fish. collection_date: 10-Oct-2004. collected_by: Juan Fuentes and Carla Viegas

text: adult

text: Sparus aurata

text: male

text: testis


status: PUBLIC

characteristic: External Id

text: MPI for Molecular Genetics

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Total RNA was extracted from 5 male specimens of weight ranging between 400 and 500 g. Collected tissue frozen in liq N2 and stored at -80C prior to RNA extraction. mRNA purification and concentration were performed using the Dynal's oligo-dT magnetic beads. Library construction was performed with Creator SMART cDNA library construction kit (Clontech) and the thermostable duplex-specific nuclease (Zhu et al., 2001). Initial LD PCR amplification of 22 cycles for enrichment of full length transcripts was verified on Agarose gels. cDNA sizes (>500 base pairs) were selected using filter columns based on agarose gels as described in the Clontech manual. Library construction and sequencing were performed at the MPI of Molecular Genetics Berlin. isolation_source: aquaculture fish. collection_date: 10-Oct-2004. collected_by: Juan Fuentes and Carla Viegas

characteristic: description

text: adult

tag: attribute

characteristic: dev-stage

text: Dicentrarchus labrax

characteristic: organism

text: male

characteristic: sex

text: trunk kidney

characteristic: tissue-type

characteristic: title


text: MPI for Molecular Genetics

text: live

text: Generic

text: adult

text: Dicentrarchus labrax

text: male

text: intestine


text: MPI for Molecular Genetics

text: live

text: Generic

text: The library was made from a suppression subtractive hybridisation (SSH): mRNA extracted from pool of whole larvae at day10 post hatching subtracted by mRNA extracted from pool of juveniles (day50 post hatching). Collected larvae were frozen in liq N2 and stored at -80C prior to RNA extraction. mRNA purifications were performed using oligotex mRNA midi kit (QIAGEN). Library construction was performed with PCR-select cDNA Substraction Kit (Clontech) and the Advantage cDNA polymerase Mix (Clontech). PCR products were inserted in pCR(R) II-TOPO(R) (Invitrogen) and cloned in XL 10-Gold(R) Kanr Ultracompetent Cells (Stratagene). Library sequencing was performed at the MPI of Molecular Genetics Berlin. isolation_source: aquaculture fish. collection_date: 2006. collected_by: Maria Darias Caceres and David Mazurais

text: larvae

text: Dicentrarchus labrax

text: undifferentiated

text: whole larvae


text: MPI for Molecular Genetics

text: live

text: Generic

text: adult

text: Dicentrarchus labrax

text: male

text: muscle


text: College of Life Science and Technology, Southwest University for Nationalities

text: live

text: Generic

text: colostrum lactating

text: Bos grunniens

text: Maiwa yak

characteristic: strain

text: cDNA subtractive library of mammary gland tissue of yak at its colostrum lactating stage

text: Plasmid

characteristic: vector


text: Forest protection of Nanjing Forestry University

text: live

text: Generic

text: The SSH was performed with cDNA from Bursaphelenchus mucronatus strain AN5#F7-1 as the tester and B. xylophilus AN19#F1-1 as the driver. Tester and driver cDNA were digested with Rsa I, the digested tester cDNA was divided into two sub- populations and each one was ligated with one of the two adaptors provided (adaptor 1 and adaptor 2R), followed by heat denaturation of both tester and driver cDNA populations. Two hybridizations were performed to generate templates for PCR amplification. An excess of driver was added to each sample of the tester for the first hybridization reaction, leading to equalization and enrichment of differentially expressed sequences. Products of each tester sub- population (adaptor 1 and adaptor 2R) from the first hybridization were mixed and immediately subjected to a second hybridization in which fresh denatured driver cDNA was added to further enrich the differential expressed sequences. Differential expressed sequences were selectively amplified by two rounds of PCR using primers specific to the adaptors, resulting in suppression of sequences common to the two cDNA populations and exponential amplification of the differentially expressed sequences. The subtracted PCR products were purified and then cloned into the pGEM-T easy vector. The ligated DNA was transformed into Escherichia coli JM109 competent cells. Clones were plated on LB medium supplemented with X-gal, IPTG and ampicillin. Individual recombinant white clones were picked and grown in LB medium containing ampicillin on 96-well plates to get the SSH library of B. mucronatus especially expressed.

text: Mixed stage

characteristic: isolate

characteristic: lab-host

text: Bursaphelenchus xylophilus

text: SSH cDNA library of Bursaphelenchus mucronatus especially expressed

text: pGEM-T easy vector


characteristic: External Id

text: live

text: Generic

text: The SSH was performed with cDNA from Bursaphelenchus xylophilus strain AN19#F1-1 as the tester and B.mucronatus strain AN5#F7-1 as the driver. Tester and driver cDNA were digested with Rsa I, the digested tester cDNA was divided into two sub-populations and each one was ligated with one of the two adaptors provided (adaptor 1 and adaptor 2R), followed by heat denaturation of both tester and driver cDNA populations. Two hybridizations were performed to generate templates for PCR amplification. An excess of driver was added to each sample of the tester for the first hybridization reaction, leading to equalization and enrichment of differentially expressed sequences. Products of each tester sub-population (adaptor 1 and adaptor 2R) from the first hybridization were mixed and immediately subjected to a second hybridization in which fresh denatured driver cDNA was added to further enrich the differential expressed sequences. Differential expressed sequences were selectively amplified by two rounds of PCR using primers specific to the adaptors, resulting in suppression of sequences common to the two cDNA populations and exponential amplification of the differentially expressed sequences. The subtracted PCR products were purified and then cloned into the pGEM-T easy vector. The ligated DNA was transformed into Escherichia coli JM109 competent cells. Clones were plated on LB medium supplemented with X-gal, IPTG and ampicillin. Individual recombinant white clones were picked and grown in LB medium containing ampicillin on 96-well plates to get SSH library of B. xylophilus especially expressed.

text: Mixed stage

text: Bursaphelenchus xylophilus

text: SSH cDNA library of Bursaphelenchus xylophilus especially expressed

text: pGEM-T easy vector


text: National Institute of Agricultural Biotechnology

characteristic: INSDC center name

text: live

text: Generic

text: Yeoung San

characteristic: cultivar

text: Using annealing control primer system, differentially expressed genes (DEG) (tissue specifically or seed development specifically) in Brassica napus was identified. The DEG products was isolated and inserted into pGEM-T Easy vector

text: E.coli

text: Brassica napus

text: EcoRI

text: DEG in Brassica napus using annealing control primer system

text: pGEM T-Easy vector


text: University of New Hampshire

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Mixed

characteristic: cell type

text: The descending aorta from one to three day-old atherosclerosis-resistant Show Racer (SR) pigeons and atherosclerosis-susceptible (spontaneous, non-induced) White Carneau (WC) pigeons were used to determine differences in genetic expression between the two breeds. Total RNA was extracted (Trizol, Invitrogen), purified, and converted to double-stranded cDNA using the BD SMART PCR cDNA Synthesis Kit (Clontech). Full-length cDNA from each pigeon strain was cut with DpnII (5'GATC) in order to prepare amplicons for comparison by Representational Difference Analysis (RDA). cDNA fragments were subjected to three rounds of PCR-coupled subtractive hybridization and the final difference products were cloned into the BamHI restriction site of the pBluescript SK+ phagemid vector and transformed into XL1 MRF- chemically competent host cells (Stratagene). Transcripts were purified from the host cells by Rolling Circle Amplification (GE Healthcare) and sequenced by the DNAcore facility at the Hubbard Center for Genome Studies, University of New Hampshire. Sequences represent genes that were differentially expressed between the two strains. The SR175 library contains transcripts that were upregulated in SR aortic tissue relative to the corresponding site in the WC aorta. The corresponding WC library (WC175) contains transcripts that were upregulated in the WC aortic tissue relative to SR175.

text: XL1 MRF-

text: Columba livia

text: BAMHI

text: Mixed

text: White Carneau

text: Descending Aorta

text: pBluescript SK+


text: University of New Hampshire

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Mixed

text: The descending aorta from one to three day-old atherosclerosis-resistant Show Racer (SR) pigeons and atherosclerosis-susceptible (spontaneous, non-induced) White Carneau (WC) pigeons were used to determine differences in genetic expression between the two breeds. Total RNA was extracted (Trizol, Invitrogen), purified, and converted to double-stranded cDNA using the BD SMART PCR cDNA Synthesis Kit (Clontech). Full-length cDNA from each pigeon strain was cut with DpnII (5'GATC) in order to prepare amplicons for comparison by Representational Difference Analysis (RDA). cDNA fragments were subjected to three rounds of PCR-coupled subtractive hybridization and the final difference products were cloned into the BamHI restriction site of the pBluescript SK+ phagemid vector and transformed into XL1 MRF- chemically competent host cells (Stratagene). Transcripts were purified from the host cells by Rolling Circle Amplification (GE Healthcare) and sequenced by the DNAcore facility at the Hubbard Center for Genome Studies, University of New Hampshire. Sequences represent genes that were differentially expressed between the two strains. The WC175 library contains transcripts that were upregulated in WC aortic tissue relative to the corresponding site in the SR aorta. The corresponding SR library (SR175) contains transcripts that were upregulated in the SR aortic tissue relative to WC175.

text: XL1 MRF-

text: Columba livia

text: BAMHI

text: Mixed

text: White Carneau

text: Descending Aorta

text: pBluescript SK+


text: Institution of Special Wild Economic Animal and Plant, CAA

text: live

text: Generic

text: mesenchymocyte

text: mRNA was isolated from mesenchymal tissues of antler and applyed cDNA-AFLP technique to obtain the differential expression genes. Then cloned these ESTs using T/A cloning kit and sequenced.

characteristic: description

text: antler

characteristic: organ

text: Cervus nippon

characteristic: organism

text: male

text: mesenchyme

text: antler mesenchymal tissue library

text: Cosmid


text: Faculdade de Medicina

text: live

text: Generic

text: Secretory

text: PCR-based cDNA library using SMART cDNA library construction kit from Clontech

text: Salivary gland

text: Panstrongylus megistus

text: Salivary gland

characteristic: tissue-type

text: Panstrongylus megistus salivary gland cDNA library

characteristic: title

text: Lambda TriplEx2


text: University of Algarve, Centre of Marine Sciences

text: live

text: Generic

text: Total RNA was extracted from intervetebral tissue. The cDNA was cloned into pBluescript using UNI-ZAP XR Vector (Stratagene)

text: Juvenile

characteristic: dev-stage

text: Aquaculture fish

text: Sparus aurata

text: Undifferentiated

characteristic: sex

text: Intervetebral cartilage

text: Sparus aurata EST, intervetebral tissue


text: live

text: Generic

text: Total RNA was extracted from differentially enriched library from PTHrP and Control larval heads. Library construction was performed with Clontech PCR-Select cDNA Subtraction Kit and cloned into pGEMT easy

text: Larvae

text: Aquaculture fish

characteristic: isolate

text: Sparus aurata

text: Undifferentiated

text: Heads of larvae

text: Sparus aurata EST, heads of larvae


characteristic: External Id

text: live

text: Generic

text: Total RNA was extracted from kidney. Library construction was performed with pBluescript

text: Juvenile

text: Aquaculture fish

text: Kidney

text: Sparus aurata

text: Undifferentiated

text: Sparus aurata EST, kidney


text: RIKEN Institute

characteristic: INSDC center name

text: live

text: Generic

text: Hodotermopsis sjostedti

text: salivary gland

text: Hodotermopsis sjoestedti salivary gland


text: RIKEN Institute

text: live

text: Generic

text: Hodotermopsis sjostedti

text: foregut

text: Hodotermopsis sjoestedti foregut


text: RIKEN Institute

text: live

text: Generic

text: Hodotermopsis sjostedti

text: whole body

text: Hodotermopsis sjoestedti whole body


text: RIKEN Institute

text: live

text: Generic

text: Hodotermopsis sjostedti

text: midgut

text: Hodotermopsis sjoestedti midgut


text: RIKEN Institute

text: live

text: Generic

text: Hodotermopsis sjostedti

text: hindgut

text: Hodotermopsis sjoestedti hindgut


text: USDA-ARS

text: live

text: Generic

text: Leptinotarsa decemlineata

text: Differential display screening of prediapausing Leptinotarsa decemlineata


text: Hainan University

text: live

text: Generic

text: Pinctada imbricata

text: cDNA library in Pinctada martensi


text: University of New Hampshire

text: live

text: Generic

text: Mixed

text: The descending aorta from one to three day-old atherosclerosis-resistant Show Racer (SR) pigeons and atherosclerosis-susceptible (spontaneous, non-induced) White Carneau (WC) pigeons were used to determine differences in genetic expression between the two breeds. Total RNA was extracted (Trizol, Invitrogen), purified, and converted to double-stranded cDNA using the BD SMART PCR cDNA Synthesis Kit (Clontech). Full-length cDNA from each pigeon strain was cut with DpnII (5'GATC) in order to prepare amplicons for comparison by Representational Difference Analysis (RDA). cDNA fragments were subjected to three rounds of PCR-coupled subtractive hybridization and the final difference products were cloned into the BamHI restriction site of the pBluescript SK+ phagemid vector and transformed into XL1 MRF- chemically competent host cells (Stratagene). Transcripts were purified from the host cells by Rolling Circle Amplification (GE Healthcare) and sequenced by the DNAcore facility at the Hubbard Center for Genome Studies, University of New Hampshire. Sequences represent genes that were differentially expressed between the two strains. The SR180 library contains transcripts that were upregulated in SR aortic tissue relative to the corresponding site in the WC aorta. The corresponding WC library (WC180) contains transcripts that were upregulated in the WC aortic tissue relative to SR180.

text: XL1 MRF-

characteristic: lab-host

text: Columba livia

text: BAMHI

text: Mixed

text: White Carneau

characteristic: strain

text: Descending Aorta

text: pBluescript SK+

characteristic: vector


text: University of New Hampshire

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Mixed

characteristic: cell type

text: The descending aorta from one to three day-old atherosclerosis-resistant Show Racer (SR) pigeons and atherosclerosis-susceptible (spontaneous, non-induced) White Carneau (WC) pigeons were used to determine differences in genetic expression between the two breeds. Total RNA was extracted (Trizol, Invitrogen), purified, and converted to double-stranded cDNA using the BD SMART PCR cDNA Synthesis Kit (Clontech). Full-length cDNA from each pigeon strain was cut with DpnII (5'GATC) in order to prepare amplicons for comparison by Representational Difference Analysis (RDA). cDNA fragments were subjected to three rounds of PCR-coupled subtractive hybridization and the final difference products were cloned into the BamHI restriction site of the pBluescript SK+ phagemid vector and transformed into XL1 MRF- chemically competent host cells (Stratagene). Transcripts were purified from the host cells by Rolling Circle Amplification (GE Healthcare) and sequenced by the DNAcore facility at the Hubbard Center for Genome Studies, University of New Hampshire. Sequences represent genes that were differentially expressed between the two strains. The WC180 library contains transcripts that were upregulated in WC aortic tissue relative to the corresponding site in the SR aorta. The corresponding SR library (SR180) contains transcripts that were upregulated in the SR aortic tissue relative to WC180.

text: XL1 MRF-

text: Columba livia

text: BAMHI

text: Mixed

text: White Carneau

text: Descending Aorta

text: pBluescript SK+


text: University of Calgary

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Louisiana

characteristic: cultivar

text: Stem

text: Papaver somniferum

text: EcoRI

text: XhoI

text: Opium poppy stem cDNA library

text: Phage

text: Lambda ZAP; pBluescript


text: University of Calgary

text: live

text: Generic

text: Louisiana

text: Root

characteristic: organ

text: Papaver somniferum

characteristic: organism

text: EcoRI

text: XhoI

text: Opium poppy root cDNA library

text: Phage

text: Lambda ZAP; pBluescript


text: Korea Forest Research Institute

text: live

text: Generic

text: We identified genes induced by the infection of pinewood nematode (Bursaphelenchus xylophilus (Steiner et Buhrer) Nickle) in Japanese red pine (Pinus densiflora Siebold et Zucc.) by Suppression Subtractive Hybridization.

characteristic: description

text: E. coli DH10B

text: stem

text: Pinus densiflora

text: EcoRI

text: PD_BX library

characteristic: title

text: plasmid

text: pGEM-T Easy Vector


text: Korea Forest Research Institute

text: live

text: Generic

text: We identified genes induced by the infection of pitch canker pathogen (Fusarium circinatum) in hybrid pines (Pinus rigida x P. rigitaeda) by Suppression Subtractive Hybridization.

text: E. coli DH10B

text: stem

text: Pinus rigida x Pinus x rigitaeda

text: EcoRI

text: PR_FC library

text: plasmid

text: pGEM-T Easy Vector


text: Zhejiang University

text: live

text: Generic

text: SSH library preparation from tomato fruit treated with biocontrol yeast Cryptococcus laurentii

text: MG4 (mature green)

characteristic: dev-stage

text: tomato fruit

text: Solanum lycopersicum

text: tomato fruit Subtractive suppression hybridization (SSH) library


characteristic: External Id

text: National Rehabilitation Center for Persons with Disabilities, Research Institute

text: live

text: Generic

text: retinal pigment epithelium

characteristic: cell-line

text: Homo sapiens

text: pKA1U5/ARPE-19 vector-capped full-length cDNA


characteristic: INSDC center name

text: live

text: Generic

text: retinal pigment epithelium

text: Homo sapiens

text: pGCAP10/ARPE-19 vector-capped full-length cDNA


text: Universiti Putra Malaysia

text: live

text: Generic

text: Garcinia mangostana

text: RsaI

text: young flower 1-1.5cm

characteristic: tissue-type

text: Garcinia mangostana cDNA Library

text: pCR 4-TOPO


text: College of Horticulture, Kerala Agricultural University

text: live

text: Generic

text: Kalluvally

text: ESTs were developed through SSH between control and water stressed black pepper plants, variety Kalluvally. Kalluvally is a relatively drought tolerant cultivar of Piper nigrum.

text: Piper nigrum

text: RsaI

text: RsaI

text: Monecious

characteristic: sex

text: Leaf

text: Development of ESTs in black pepper var: Kalluvally through SSH

text: pGEMT

characteristic: vector


text: Shiraz University

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Seedlings of Alvand wheat were grown in growth chamber for 12 days. Isolation was carried out from leaves and by specific primers via PCR.

text: Leaf

text: Triticum aestivum

text: Alvand

characteristic: strain

text: Triticum aestivum Alvand 12 days seedlings under normal condition


text: Shiraz University

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Seedlings of Bayat wheat were grown in growth chamber for 12 days. Isolation was carried out from leaves and by specific primers via PCR.

text: Leaf

text: Triticum aestivum

text: Bayat

text: Triticum aestivum Bayat 12 days seedlings under normal condition


text: Shiraz University

text: live

text: Generic

characteristic: NCBI submission package

text: Seedlings of Besostaya wheat were grown in growth chamber for 12 days. Isolation was carried out from leaves and by specific primers via PCR.

text: Leaf

text: Triticum aestivum

text: Besostaya

text: Triticum aestivum Besostaya 12 days seedlings under normal condition


text: Shiraz University

text: live

text: Generic

text: Seedlings of Chamran wheat were grown in growth chamber for 12 days. Isolation was carried out from leaves and by specific primers via PCR.

text: Leaf

characteristic: organ

text: Triticum aestivum

text: Chamran

text: Triticum aestivum Chamran 12 days seedlings under normal condition


text: Shiraz University

text: live

text: Generic

text: Seedlings of Darab wheat were grown in growth chamber for 12 days. Isolation was carried out from leaves and by specific primers via PCR.

text: Leaf

text: Triticum aestivum

characteristic: organism

text: Darab

text: Triticum aestivum Darab 12 days seedlings under normal condition


text: Shiraz University

text: live

text: Generic

text: Seedlings of Mahouti wheat were grown in growth chamber for 12 days. Isolation was carried out from leaves and by specific primers via PCR.

characteristic: description

text: Leaf

text: Triticum aestivum

text: Mahouti

text: Triticum aestivum Mahouti 12 days seedlings under normal condition


text: Shiraz University

text: live

text: Generic

text: Seedlings of Omid wheat were grown in growth chamber for 12 days. Isolation was carried out from leaves and by specific primers via PCR.

text: Leaf

text: Triticum aestivum

text: Omid

text: Triticum aestivum Omid 12 days seedlings under normal condition

characteristic: title


text: Shiraz University

text: live

text: Generic

text: Seedlings of Roushan wheat were grown in growth chamber for 12 days. Isolation was carried out from leaves and by specific primers via PCR.

characteristic: dev-stage

text: Leaf

text: Triticum aestivum

text: Roushan

text: Triticum aestivum Roushan 12 days seedlings under normal condition


characteristic: External Id

text: Shiraz University

text: live

text: Generic

text: Seedlings of Sardari wheat were grown in growth chamber for 12days. Isolation was carried out from leaves and by specific primers via PCR.

text: Leaf

text: Triticum aestivum

text: Sardari

text: Triticum aestivum Sardari 12 days seedlings under normal condition


text: Shiraz University

text: live

text: Generic

text: Seedlings of Shahi wheat were grown in growth chamber for 12 days. Isolation was carried out from leaves and by specific primers via PCR.

text: Leaf

text: Triticum aestivum

text: Shahi

text: Triticum aestivum Shahi 12 days seedlings under normal condition


text: Shiraz University

text: live

text: Generic

text: Seedlings of Zarrin wheat were grown in growth chamber for 12 days. Isolation was carried out from leaves and by specific primers via PCR.

text: Leaf

text: Triticum aestivum

text: Zarrin

text: Triticum aestivum Zarrin 12 days seedlings under normal condition


text: Northwest A&F University

text: live

text: Generic

characteristic: cultivar

text: cDNA-AFLP analysis reveals differential gene expression in incompatible reaction of wheat challenged with Puccinia striiformis f. sp. tritici

text: The first leaf of seven-day old wheat seedlings

text: Triticum aestivum

text: leaf

text: Transcript derived fragments (TDFs) from wheat leaves inoculated by stripe rust fungus (CY23)

text: pGEM-T easy


text: Brigham Young University

characteristic: INSDC center name

text: live

text: Generic

text: Creator SMART cDNA Library Construction Kit from Clonetech using pDNR_Lib plasmid vector

text: Mixed

text: Plectus murrayi

text: Whole nematode

text: Plectus murrayi desiccated cDNA library


text: The Third Military Medical University

text: live

text: Generic

text: country: China

text: Mus musculus

text: BALB/c

text: Mus musculus BALB/c China


text: Hunan Normal University

text: live

text: Generic

text: adult

text: Chilobrachys guangxiensis

text: Sfi 1

text: Sfi 1

text: female

text: Venom gland

characteristic: tissue-type

text: Spider venom gland Library

text: Plasmid

text: pDNR-LIB


text: Kyoto Institute of Technology

text: live

text: Generic

text: adult

text: Drosophila simulans

text: male

characteristic: sex

text: Rakujuen RM13

text: whole body

text: Drosophila full-length cDNA library, Drosophila simulans whole body adult male


text: Kyoto Institute of Technology

text: live

text: Generic

text: adult

text: Drosophila auraria

text: mixed

text: whole body

text: Drosophila full-length cDNA library, Drosophila auraria whole body adult mixed


text: Kyoto Institute of Technology

text: live

text: Generic

text: adult

text: Drosophila sechellia

text: mixed

text: whole body

text: Drosophila full-length cDNA library, Drosophila sechellia whole body adult mixed


text: Kyoto Institute of Technology

text: live

text: Generic

text: adult

text: Drosophila simulans

text: female

text: Rakujuen RM13

text: whole body

text: Drosophila full-length cDNA library, Drosophila simulans whole body adult female


text: Kyoto Institute of Technology

text: live

text: Generic

text: third instar larva

text: Drosophila simulans

text: mixed

text: Rakujuen RM13

text: whole body

text: Drosophila full-length cDNA library, Drosophila simulans whole body third instar larva mixed


text: The University of Tokyo, Laboratory of Insect Genetics and Bioscience

text: live

text: Generic

text: fifth instar larvae

text: Samia ricini

text: fat body

text: IND09 (fat body of Escherichia coli-challenged fifth-instar larvae)

text: pBluescript II SK+

characteristic: vector


characteristic: INSDC first public

text: live

text: Generic

text: fifth-instar larvae

text: Samia ricini

text: fat body

text: IND10 (fat body of Candida albicans-challenged fifth-instar larvae)

text: pBluescript II SK+


characteristic: INSDC last update

text: live

text: Generic

text: 96 hours after oviposition

text: Samia ricini

text: embryo

text: SMD06 (Embryos 96 hours after oviposition)

text: pBluescript II SK+


text: FFCLRP, University of Sao Paulo

text: live

text: Generic

text: Petit Havana SR1

text: flower developmental stages 1 to 11

text: stigmas/styles

text: Nicotiana tabacum

text: TOBEST cDNA library 1

text: Plasmid


text: Advanced Technologies (Cambridge)

text: live

text: Generic

text: This library contains tobacco leaf ESTs origninating from either the Samsun trichome, Burley21 trichome, K326 trichome, K326 early senescent leaf, or K326 late senescent leaf libraries.

text: Leaf

text: Nicotiana tabacum

text: tobacco leaf library


text: Advanced Technologies (Cambridge)

text: live

text: Generic

text: Plants were grown on soil in glasshouses under long days (16h:8h). RNA was extracted from trichomes based on the method of Aziz et al. (2004). Briefly, stems from 30 plants were cut into 5-10cm fragments and flash frozen in liquid nitrogen. Segments were individually shaken in pre-frozen 50ml platic tubes to release the trichomes. Collected trichomes were stored in Tri Reagent (Sigma) and RNA was extracted according to manufacturers instructions.

text: Leaf

text: Nicotiana tabacum

text: Samsun

characteristic: strain

text: trichome

text: Samsun trichome library


text: Advanced Technologies (Cambridge)

text: live

characteristic: INSDC status

text: Generic

text: Leaf

text: Nicotiana tabacum

text: Burley

text: trichome

text: Burley21 trichome library


text: Advanced Technologies (Cambridge)

text: live

text: Generic

characteristic: NCBI submission model

text: Leaf

text: Nicotiana tabacum

text: Virginia

text: trichome

text: K326 trichome library


text: Advanced Technologies (Cambridge)

text: live

text: Generic

characteristic: NCBI submission package

text: Total RNA was extracted from the harvested plant material according to the method of Geurche et al. (1990), followed by purification of poly A+ mRNA using DynalBeads according to the manufacturers protocol (Dynal).

text: Leaf

text: Nicotiana tabacum

text: Virginia

text: K326 early senescent leaf library


text: Advanced Technologies (Cambridge)

text: live

text: Generic

text: Leaf

characteristic: organ

text: Nicotiana tabacum

text: Virginia

text: K326 late senescent leaf library


text: Universidad de los Andes

text: live

text: Generic

text: Mixed Col2 (Susceptible) and Col3 (Resistant)

text: Four week old plants

text: Solanum phureja

characteristic: organism

text: Leaves

text: Healthy leaf tissue

text: Plasmid

text: modified pAL17.3


text: Broad University

text: live

text: Generic

text: Brain from anolis lizard IDs #26&27 from Camilla T. Peabody in Dr. Juli Wade's lab (Michigan State.) Library constructed by Zach Smith and John K. Colbourne (Indiana University.)

characteristic: description

text: Brain

text: Anolis carolinensis

text: Sfi I A

text: Sfi I B

text: Whole Brain

text: Anolis carolinensis pooled normalized Brain cDNA Library

text: Plasmid

text: pDNRLib


text: Broad University

text: live

text: Generic

text: Dewlap from anolis lizard ID #27 from Camilla T. Peabody in Dr. Juli Wade's lab (Michigan State.) Library constructed by Zach Smith and John K. Colbourne (Indiana University.)

text: Dewlap

text: Anolis carolinensis

text: Sfi I A

text: Sfi I B

text: Dewlap

text: Anolis carolinensis pooled normalized Dewlap cDNA Library

characteristic: title

text: Plasmid

text: pDNRLib


text: Broad University

text: live

text: Generic

text: Embryo of anolis lizard ID #29 from Camilla T. Peabody in Dr. Juli Wade's lab (Michigan State.) Library constructed by Zach Smith and John K. Colbourne (Indiana University.)

text: Embryo

text: Anolis carolinensis

text: Sfi I A

text: Sfi I B

text: Embryo

text: Anolis carolinensis pooled normalized embryo cDNA library

text: Plasmid

text: pDNRLib


text: Broad University

text: live

text: Generic

text: Mixed organs of anolis lizard IDs #26&27 from Camilla T. Peabody in Dr. Juli Wade's lab (Michigan State.) Library constructed by Zach Smith and John K. Colbourne (Indiana University.)

text: Mixed

text: Anolis carolinensis

text: Sfi I A

text: Sfi I B

text: Tongue, liver, gallbladder, spleen, heart, kidney, lung

text: Anolis carolinensis pooled normalized mixed organ cDNA library

text: Plasmid

text: pDNRLib


text: Broad University

text: live

text: Generic

text: Ovary of anolis lizard ID #656 from Camilla T. Peabody in Dr. Juli Wade's lab (Michigan State.) Library constructed by Zach Smith and John K. Colbourne (Indiana University.)

text: Ovary

text: Anolis carolinensis

text: Sfi I A

text: Sfi I B

text: Ovary

text: Anolis carolinensis pooled normalized ovary cDNA library

text: Plasmid

text: pDNRLib


text: Broad University

text: live

text: Generic

text: Regenerating tail of anolis lizard ID #s 605 & 610 from Camilla T. Peabody in Dr. Juli Wade's lab (Michigan State.) Library constructed by Zach Smith and John K. Colbourne (Indiana University.)

text: Regenerating tail

text: Anolis carolinensis

text: Sfi I A

text: Sfi I B

text: Regenerating tail

text: Anolis carolinensis pooled normalized regenerating tail cDNA library

text: Plasmid

text: pDNRLib


text: Broad University

text: live

text: Generic

text: Testes of anolis lizard ID #27 from Camilla T. Peabody in Dr. Juli Wade's lab (Michigan State.) Library constructed by Zach Smith and John K. Colbourne (Indiana University.)

text: Testes

text: Anolis carolinensis

text: Sfi I A

text: Sfi I B

text: Testes

text: Anolis carolinensis pooled normalized testes cDNA library

text: Plasmid

text: pDNRLib


text: University of Amsterdam

text: live

text: Generic

text: Agaricus bisporus

text: Horst U1

text: mushroom primordia

text: Agaricus bisporus mushroom primordia Horst U1


text: Shiraz University

text: live

text: Generic

text: Alhagi pseudalhagi were harvested near Shiraz city. Isolation was carried out from leaves and by specific primers via PCR.

text: Plants under drought condition

characteristic: dev-stage

text: Leaf

text: Alhagi maurorum

text: Alhagi pseudalhagi gene isolation method


characteristic: External Id

text: National Floriculture Engineering Research Center of Beijing Forestry University

text: live

text: Generic

text: zilian

text: ESTs from chrysanthemum morifolium innoculated by Puccinia horiana obtained by cDNA-AFLP. These ESTs were differently expressed in the cultivar zilian between innoculated and non-innoculated plants.

text: Chrysanthemum x morifolium

text: Leaves

text: Chrysanthemum innoculated by Puccinia horiana library


text: University of California

text: live

text: Generic

characteristic: cultivar

text: Seeds of 524B were from a single plant with a long history of single seed descent (Ehlers)from a California breeding program. The types of tissue were: 1) germinated seed hypocotyl and primary root, 2) germinated seed epicotyl and foliage leaves, 3 & 4) young trifoliate leaves and apical meristem (one well watered, one drought stressed). For germinated seed samples (1 & 2), seeds were surface sterilized 5 minutes in 1% sodium hypochlorite with 0.01% Tween 20, rinsed with water, germinated on sterile filter paper with water containing 100 units/ml nystatin and 50 microgram/ml cefotaxime, incubated 4-5 days in the dark at room temperature (Fenton). For samples 3 & 4, seeds were planted in 3 gallon pots and kept watered in a glasshouse until plants were ~25 cm in height (~3 weeks)(Fenton). For drought stress (sample 4), water was withheld until visible signs of drought stress occurred (reduced growth relative to watered plants, wilted leaves, less green color), generally ~3 weeks. Total RNA was purified using a TRIzol method, except Qiagen RNeasy for hypocotyls & root (Fenton). RNA was pooled to establish one complex RNA sample with a total of 400 microgram, as follows: 133 microgram of hypocotyl & primary root (sample 1), 133 microgram of epicotyl and foliage leaves (sample 2) and 67 microgram each of stressed and unstressed young trifoliate leaves and apical meristem (samples 3 & 4)(Fenton). PolyA RNA was purified using Absolutely mRNA Purification Kit (Stratagene)(Fenton). 1.7 microgram of poly(A) mRNA was used for cDNA synthesis. A primary cDNA library with a titer of 10.9 million cfu (Close) was produced using a SuperScript Plasmid System with Gateway Technology for cDNA Synthesis and Cloning (Invitrogen)(Fenton). Plating and sequencing was performed at the US Department of Energy Joint Genome Institute Sequence Production Facility, Walnut Creek California (Bristow, Wang, Lucas, Lindquist, Pennacchio). The library was plated onto agarose plates containing ampicillin (254 mm plates from Teknova, Hollister, CA) at a density of approximately 1000 colonies per plate. Plates were grown at 37 degrees Celsius for 18 hours, then individual colonies were picked and each used to inoculate a well containing LB media with ampicillin in a 384 well plate (Nunc, Rochester, NY). 384 well plates were grown at 37 degrees Celsius for 18 hours. Plasmid DNA for sequencing was produced by rolling circle amplification (Templiphi, GE Healthcare, Piscataway, NJ). Subclone inserts were sequenced from both ends using primers complementary to the flanking pCMV-SPORT6 vector sequence and Big Dye terminator chemistry and then run on ABI 3730 instruments (ABI, Foster City, CA). Sequencing protocols can be found in Detter et al (2002, Genomics 80:691-698) and at http://www.jgi.doe.gov/sequencing/protocols/index.html. Trace files were downloaded (Close) and then processed (Wanamaker) using the HarvEST pipeline (http://harvest.ucr.edu) to remove vector and cloning oligo sequences and various contaminants, and to trim to a high quality region. Sequences that retained a phred 17 region of at least 100 bases were deposited to GenBank with annotations (Close).

text: Seedling

text: E. coli DH10B

characteristic: lab-host

text: Vigna unguiculata

text: SalI

text: NotI

text: mixed

text: Cowpea 524B Mixed Tissue and Conditions cDNA Library UCRVU04-1-2

text: Plasmid


text: University of California

characteristic: INSDC center name

text: live

text: Generic

text: Seeds of IT84S-2049 were from a single plant with a long history of single seed descent (Ehlers) from a Nigerian breeding program. The types of tissue were: 1) germinated seed hypocotyl and primary root, 2) germinated seed epicotyl and foliage leaves, 3 & 4) young trifoliate leaves and apical meristem (one well watered, one drought stressed). For germinated seed samples (1 & 2), seeds were surface sterilized 5 minutes in 1% sodium hypochlorite with 0.01% Tween 20, rinsed with water, germinated on sterile filter paper with water containing 100 units/ml nystatin and 50 microgram/ml cefotaxime, incubated 4-5 days in the dark at room temperature (Fenton). For samples 3 & 4, seeds were planted in 3 gallon pots and kept watered in a glasshouse until plants were ~25 cm in height (~3 weeks)(Fenton). For drought stress (sample 4), water was withheld until visible signs of drought stress occurred (reduced growth relative to watered plants, wilted leaves, less green color), generally ~3 weeks. Total RNA was purified using a TRIzol method, except Qiagen RNeasy for hypocotyls & root (Fenton). RNA was pooled to establish one complex RNA sample with a total of 400 microgram, as follows: 133 microgram of hypocotyl & primary root (sample 1), 133 microgram of epicotyl and foliage leaves (sample 2) and 67 microgram each of stressed and unstressed young trifoliate leaves and apical meristem (samples 3 & 4)(Fenton). PolyA RNA was purified using Absolutely mRNA Purification Kit (Stratagene)(Fenton). 3.0 microgram of poly(A) mRNA was used for cDNA synthesis. A primary cDNA library with a titer of 17.6 million cfu (Close) was produced using a SuperScript Plasmid System with Gateway Technology for cDNA Synthesis and Cloning (Invitrogen)(Fenton). Plating and sequencing was performed at the US Department of Energy Joint Genome Institute Sequence Production Facility, Walnut Creek California (Bristow, Wang, Lucas, Lindquist, Pennacchio). The library was plated onto agarose plates containing ampicillin (254 mm plates from Teknova, Hollister, CA) at a density of approximately 1000 colonies per plate. Plates were grown at 37 degrees Celsius for 18 hours, then individual colonies were picked and each used to inoculate a well containing LB media with ampicillin in a 384 well plate (Nunc, Rochester, NY). 384 well plates were grown at 37 degrees Celsius for 18 hours. Plasmid DNA for sequencing was produced by rolling circle amplification (Templiphi, GE Healthcare, Piscataway, NJ). Subclone inserts were sequenced from both ends using primers complementary to the flanking pCMV-SPORT6 vector sequence and Big Dye terminator chemistry and then run on ABI 3730 instruments (ABI, Foster City, CA). Sequencing protocols can be found in Detter et al (2002, Genomics 80:691-698) and at http://www.jgi.doe.gov/sequencing/protocols/index.html. Trace files were downloaded (Close) and then processed (Wanamaker) using the HarvEST pipeline (http://harvest.ucr.edu) to remove vector and cloning oligo sequences and various contaminants, and to trim to a high quality region. Sequences that retained a phred 17 region of at least 100 bases were deposited to GenBank with annotations (Close).

text: Seedling

text: E. coli DH10B

text: Vigna unguiculata

text: SalI

text: NotI

text: mixed

characteristic: tissue-type

text: Cowpea IT84S-2049 Mixed Tissue and Conditions cDNA Library UCRVU05-2-2

text: Plasmid


text: University of California

text: live

text: Generic

text: UCR 779

text: Seeds of UCR 779 were from a single plant with a long history of single seed descent (Ehlers) from Botswana. The types of tissue were: 1) germinated seed hypocotyl and primary root, 2) germinated seed epicotyl and foliage leaves, 3 & 4) young trifoliate leaves and apical meristem (one well watered, one drought stressed). For germinated seed samples (1 & 2), seeds were surface sterilized 5 minutes in 1% sodium hypochlorite with 0.01% Tween 20, rinsed with water, germinated on sterile filter paper with water containing 100 units/ml nystatin and 50 microgram/ml cefotaxime, incubated 4-5 days in the dark at room temperature (Fenton). For samples 3 & 4, seeds were planted in 3 gallon pots and kept watered in a glasshouse until plants were ~25 cm in height (~3 weeks)(Fenton). For drought stress (sample 4), water was withheld until visible signs of drought stress occurred (reduced growth relative to watered plants, wilted leaves, less green color), generally ~3 weeks. Total RNA was purified using a TRIzol method, except Qiagen RNeasy for hypocotyls & root (Fenton). RNA was pooled to establish one complex RNA sample with a total of 500 microgram, as follows: 167 microgram of hypocotyl & primary root (sample 1), 167 microgram of epicotyl and foliage leaves (sample 2) and 87 microgram each of stressed and unstressed young trifoliate leaves and apical meristem (samples 3 & 4)(Fenton). PolyA RNA was purified using PolyATrack mRNA Isolation System IV (Promega)(Fenton). 2.0 microgram of poly(A) mRNA was used for cDNA synthesis. A primary cDNA library with a titer of 23 million cfu (Close) was produced using a SuperScript Plasmid System with Gateway Technology for cDNA Synthesis and Cloning (Invitrogen)(Fenton). Plating and sequencing was performed at the US Department of Energy Joint Genome Institute Sequence Production Facility, Walnut Creek California (Bristow, Wang, Lucas, Lindquist, Pennacchio). The library was plated onto agarose plates containing ampicillin (254 mm plates from Teknova, Hollister, CA) at a density of approximately 1000 colonies per plate. Plates were grown at 37 degrees Celsius for 18 hours, then individual colonies were picked and each used to inoculate a well containing LB media with ampicillin in a 384 well plate (Nunc, Rochester, NY). 384 well plates were grown at 37 degrees Celsius for 18 hours. Plasmid DNA for sequencing was produced by rolling circle amplification (Templiphi, GE Healthcare, Piscataway, NJ). Subclone inserts were sequenced from both ends using primers complementary to the flanking pCMV-SPORT6 vector sequence and Big Dye terminator chemistry and then run on ABI 3730 instruments (ABI, Foster City, CA). Sequencing protocols can be found in Detter et al (2002, Genomics 80:691-698) and at http://www.jgi.doe.gov/sequencing/protocols/index.html. Trace files were downloaded (Close) and then processed (Wanamaker) using the HarvEST pipeline (http://harvest.ucr.edu) to remove vector and cloning oligo sequences and various contaminants, and to trim to a high quality region. Sequences that retained a phred 17 region of at least 100 bases were deposited to GenBank with annotations (Close).

text: Seedling

text: E. coli DH10B

text: Vigna unguiculata

text: SalI

text: NotI

text: mixed

text: Cowpea UCR 779 Mixed Tissue and Conditions cDNA Library UCRVU07-1-2

text: Plasmid

characteristic: vector


text: University of California

characteristic: INSDC first public

text: live

text: Generic

text: Seeds of IT97K-461-4 were from a single plant with a long history of single seed descent (Ehlers) from a Nigerian breeding program. The types of tissue were: 1) germinated seed hypocotyl and primary root, 2) germinated seed epicotyl and foliage leaves, 3 & 4) young trifoliate leaves and apical meristem (one well watered, one drought stressed). For germinated seed samples (1 & 2), seeds were surface sterilized 5 minutes in 1% sodium hypochlorite with 0.01% Tween 20, rinsed with water, germinated on sterile filter paper with water containing 100 units/ml nystatin and 50 microgram/ml cefotaxime, incubated 4-5 days in the dark at room temperature (Fenton). For samples 3 & 4, seeds were planted in 3 gallon pots and kept watered in a glasshouse until plants were ~25 cm in height (~3 weeks)(Fenton). For drought stress (sample 4), water was withheld until visible signs of drought stress occurred (reduced growth relative to watered plants, wilted leaves, less green color), generally ~3 weeks. Total RNA was purified using a TRIzol method, except Qiagen RNeasy for hypocotyls & root (Fenton). RNA was pooled to establish one complex RNA sample with a total of 500 microgram, as follows: 167 microgram of hypocotyl & primary root (sample 1), 167 microgram of epicotyl and foliage leaves (sample 2) and 87 microgram each of stressed and unstressed young trifoliate leaves and apical meristem (samples 3 & 4)(Fenton). PolyA RNA was purified using PolyATrack mRNA Isolation System IV (Promega)(Fenton). 2.0 microgram of poly(A) mRNA was used for cDNA synthesis. A primary cDNA library with a titer of 20 million cfu (Close) was produced using a SuperScript Plasmid System with Gateway Technology for cDNA Synthesis and Cloning (Invitrogen)(Fenton). Plating and sequencing was performed at the US Department of Energy Joint Genome Institute Sequence Production Facility, Walnut Creek California (Bristow, Wang, Lucas, Lindquist, Pennacchio). The library was plated onto agarose plates containing ampicillin (254 mm plates from Teknova, Hollister, CA) at a density of approximately 1000 colonies per plate. Plates were grown at 37 degrees Celsius for 18 hours, then individual colonies were picked and each used to inoculate a well containing LB media with ampicillin in a 384 well plate (Nunc, Rochester, NY). 384 well plates were grown at 37 degrees Celsius for 18 hours. Plasmid DNA for sequencing was produced by rolling circle amplification (Templiphi, GE Healthcare, Piscataway, NJ). Subclone inserts were sequenced from both ends using primers complementary to the flanking pCMV-SPORT6 vector sequence and Big Dye terminator chemistry and then run on ABI 3730 instruments (ABI, Foster City, CA). Sequencing protocols can be found in Detter et al (2002, Genomics 80:691-698) and at http://www.jgi.doe.gov/sequencing/protocols/index.html. Trace files were downloaded (Close) and then processed (Wanamaker) using the HarvEST pipeline (http://harvest.ucr.edu) to remove vector and cloning oligo sequences and various contaminants, and to trim to a high quality region. Sequences that retained a phred 17 region of at least 100 bases were deposited to GenBank with annotations (Close).

text: Seedling

text: E. coli DH10B

text: Vigna unguiculata

text: SalI

text: NotI

text: mixed

text: Cowpea IT97K-461-4 Mixed Tissue and Conditions cDNA Library UCRVU08-1-2

text: Plasmid


text: University of California

characteristic: INSDC last update

text: live

text: Generic

text: UCR 707

text: Seeds of UCR 707 were from a single plant with a long history of single seed descent (Ehlers) from Kenya. The types of tissue were: 1) germinated seed hypocotyl and primary root, 2) germinated seed epicotyl and foliage leaves, 3 & 4) young trifoliate leaves and apical meristem (one well watered, one drought stressed). For germinated seed samples (1 & 2), seeds were surface sterilized 5 minutes in 1% sodium hypochlorite with 0.01% Tween 20, rinsed with water, germinated on sterile filter paper with water containing 100 units/ml nystatin and 50 microgram/ml cefotaxime, incubated 4-5 days in the dark at room temperature (Fenton). For samples 3 & 4, seeds were planted in 3 gallon pots and kept watered in a glasshouse until plants were ~25 cm in height (~3 weeks)(Fenton). For drought stress (sample 4), water was withheld until visible signs of drought stress occurred (reduced growth relative to watered plants, wilted leaves, less green color), generally ~3 weeks. Total RNA was purified using a TRIzol method, except Qiagen RNeasy for hypocotyls & root (Fenton). RNA was pooled to establish one complex RNA sample with a total of 500 microgram, as follows: 167 microgram of hypocotyl & primary root (sample 1), 167 microgram of epicotyl and foliage leaves (sample 2) and 87 microgram each of stressed and unstressed young trifoliate leaves and apical meristem (samples 3 & 4)(Fenton). PolyA RNA was purified using PolyATrack mRNA Isolation System IV (Promega)(Fenton). 2.0 microgram of poly(A) mRNA was used for cDNA synthesis. A primary cDNA library with a titer of 30 million cfu (Close) was produced using a SuperScript Plasmid System with Gateway Technology for cDNA Synthesis and Cloning (Invitrogen)(Fenton). Plating and sequencing was performed at the US Department of Energy Joint Genome Institute Sequence Production Facility, Walnut Creek California (Bristow, Wang, Lucas, Lindquist, Pennacchio). The library was plated onto agarose plates containing ampicillin (254 mm plates from Teknova, Hollister, CA) at a density of approximately 1000 colonies per plate. Plates were grown at 37 degrees Celsius for 18 hours, then individual colonies were picked and each used to inoculate a well containing LB media with ampicillin in a 384 well plate (Nunc, Rochester, NY). 384 well plates were grown at 37 degrees Celsius for 18 hours. Plasmid DNA for sequencing was produced by rolling circle amplification (Templiphi, GE Healthcare, Piscataway, NJ). Subclone inserts were sequenced from both ends using primers complementary to the flanking pCMV-SPORT6 vector sequence and Big Dye terminator chemistry and then run on ABI 3730 instruments (ABI, Foster City, CA). Sequencing protocols can be found in Detter et al (2002, Genomics 80:691-698) and at http://www.jgi.doe.gov/sequencing/protocols/index.html. Trace files were downloaded (Close) and then processed (Wanamaker) using the HarvEST pipeline (http://harvest.ucr.edu) to remove vector and cloning oligo sequences and various contaminants, and to trim to a high quality region. Sequences that retained a phred 17 region of at least 100 bases were deposited to GenBank with annotations (Close).

text: Seedling

text: E. coli DH10B

text: Vigna unguiculata

text: SalI

text: NotI

text: mixed

text: Cowpea UCR 707 Mixed Tissue and Conditions cDNA Library UCRVU09-1-2

text: Plasmid


text: Jawaharlal Nehru University

text: live

text: Generic

text: trophozoite

text: clone 6

characteristic: isolate

text: Entamoeba histolytica

characteristic: strain

text: Entamoeba cDNA clone 6


text: Beijing Institute of Genomics, Chinese Academy of Sciences

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Oryza sativa Indica Group

text: Mature embryo cDNA Library of 93-11

text: Plasmid

text: pBluescriptII and PUC18


text: live

text: Generic

characteristic: NCBI submission package

text: Oryza sativa Indica Group

text: Mature embryo cDNA Library of PA64s

text: Plasmid

text: pBluescriptII and PUC18


text: live

text: Generic

text: Oryza sativa Indica Group

characteristic: organism

text: Mature embryo cDNA Library of LYP9

text: Plasmid

text: pBluescriptII and PUC18


text: ARC Seibersdorf Research GesmbH

text: live

text: Generic

text: Ziegenhorn Bello

text: Library constructed using Clontech PCR-Select cDNA Subtraction kit. Tester: leaf tissue from cultivar Ziegenhorn Bello upon early drought stress by withholding water Driver: leaf tissue from control plants;

characteristic: description

text: 6-7 leaves, youngest fully expanded leaf

text: E. coli

text: Capsicum annuum

text: leaf

text: drought stressed C. annuum leaf forward SSH library

text: plasmid

text: pCR 2.1 TOPO vector


text: ARC Seibersdorf Research GesmbH

text: live

text: Generic

text: Ziegenhorn Bello

text: Library constructed using Clontech PCR-Select cDNA Subtraction kit. Tester: leaf tissue from control plants; Driver: leaf tissue from cultivar Ziegenhorn Bello upon early drought stress by withholding water

text: E. coli

text: Capsicum annuum

text: leaf

text: drought stressed C. annuum leaf revers SSH library

characteristic: title

text: plasmid

text: pCR 2.1 TOPO vector


text: ARC Seibersdorf Research GesmbH

text: live

text: Generic

text: Ziegenhorn Bello

text: Library constructed using Clontech PCR-Select cDNA Subtraction kit. Tester: Roots from cultivar Ziegenhorn Bello upon early drought stress by withholding water; Driver: Roots from control plants

text: 6-7 leaves

characteristic: dev-stage

text: E. coli

text: Capsicum annuum

text: root

text: drought stressed C. annuum root forward SSH library

text: plasmid

text: pCR 2.1 TOPO vector


characteristic: External Id

text: ARC Seibersdorf Research GesmbH

text: live

text: Generic

text: Ziegenhorn Bello

text: Library constructed using Clontech PCR-Select cDNA Subtraction kit. Tester: roots from control plants; Driver: roots from cultivar Ziegenhorn Bello upon early drought stress by withholding water

text: 6-7 leaves

text: E. coli

text: Capsicum annuum

text: root

text: drought stressed C. annuum root revers SSH library

text: plasmid

text: pCR 2.1 TOPO vector


text: Forestry and Forest Products Research Institute

text: live

text: Generic

text: adult

text: Quercus mongolica subsp. crispula

text: inner bark

text: Quercus mongolica var. crispula inner bark cDNA library


text: Institute of Molecular and Cell Biology

text: live

text: Generic

text: adult

text: Oryzias latipes

text: testis


text: East China Normal University

text: live

text: Generic

text: Eriocheir sinensis

text: haemocytes

text: Chinese mitten crab haemocytes cDNA library

text: Plasmid

text: pBluescript SK+


text: Istituto Agrario di San Michele all'Adige (IASMA)

text: live

text: Generic

text: Pinot Noir, Moscato Bianco, Teroldego, Freiburg 933/60 (rootstock)

characteristic: cultivar

text: Total RNA from grape berry (Pinot Noir, Moscato Bianco, Teroldego, rootstock Freiburg 933/60), leaf and root (Pinot Noir) tissues were reverse transcribed in double-stranded cDNA using the SMART PCR cDNA synthesis kit (Clontech) as described by the manufacturer. A berry subtracted library was constructed using the suppression subtractive hybridization (SSH) technique performed with the PCR-Select cDNA Subtraction Kit (Clontech) according to the manufacturer's procedure. In details, after adaptor ligation, tester cDNA from berry tissue was hybridized with driver cDNA consisting of leaf and root cDNAs in equal parts. Two rounds of hybridizations and PCR amplifications were performed to enrich in berry-specific transcripts. The subtracted cDNAs amplified in the secondary PCR were cloned into the pCR 2.1-TOPO vector (Invitrogen) by means of TA cloning strategy and transformed in One Shot TOP10 Eletrocompetent E.coli (Invitrogen) cells. Positive transformants based on blue/white color selection were grown at 37 C in 384-well plates containing LB medium supplemented with 50 mg/ml kanamycin and 10% glycerol and then stored at -80 C.

text: Veraison

text: TOP10 E.coli (Invitrogen)

text: Berry

characteristic: organ

text: Vitis vinifera

text: hermaphrodite

characteristic: sex

text: deseeded berries

text: Grape berry-specific cDNA library

text: Plasmid

text: pCR 2.1-TOPO vector (Invitrogen)


text: Institute of Horticulture, Zhejiang Academy of Agricultural Sciences

characteristic: INSDC center name

text: live

text: Generic

text: Lentinula edodes

text: Lentinula edodes L98411


text: Oregon State University

text: live

text: Generic

text: Constructed from RNA extracted from 7 adult male glands with Creator Smart cDNA Library Construction Kit (BD Biosciences Clonetech)

text: Eurycea guttolineata

text: Male

text: Mental gland

characteristic: tissue-type

text: Eurycea guttolineata male mental gland library

text: Plasmid

text: pDNR-LIB

characteristic: vector


text: University College Dublin

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Frontana

text: Germinating seeds of wheat cultivar Frontana were treated with water or DON (20ug ml-1) and harvested 24h post-treatment. 1mg of extracted RNA (Ansari et al. 2007) was used to construct a phagemid lTriplEx2 vector library using the SMARTTM cDNA Library Construction kit (Clontech). Escherichia coli strain BM25.8 was used to convert lTriplEx2 to pTriplEx2 plasmid clones. The library was normalized and plasmid DNA from normalized library clones transformed into E. coli strain DH10B.

text: E. coli strain DH10B

characteristic: lab-host

text: Triticum aestivum

text: root

text: Triticum aestivum root cDNA library


text: College of Life Science, Nanjing Normal University

text: live

characteristic: INSDC status

text: Generic

text: the megalopa stage VS the first crab stage

text: Eriocheir sinensis

text: Yangtse River

characteristic: strain

text: abdomen

text: Eriocheir sinensis abdomen development subtracted library

text: Plasmid

text: pMD19-T Vector from TakaRa,Inc.


text: Norwegian University of Life Sciences

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: adult

text: Sus scrofa

text: male

text: domesticus

text: testis

text: Adult pig testis library


text: University of New Hampshire

text: live

text: Generic

text: Mixed

characteristic: cell type

text: The descending aorta from six-week old atherosclerosis-resistant Show Racer (SR) pigeons and atherosclerosis-susceptible (spontaneous, non-induced) White Carneau (WC) pigeons were used to determine differences in genetic expression between the two breeds. Total RNA was extracted (Trizol, Invitrogen), purified, and converted to double-stranded cDNA using the BD SMART PCR cDNA Synthesis Kit (Clontech). Full-length cDNA from each pigeon strain was cut with DpnII (5'GATC) in order to prepare amplicons for comparison by Representational Difference Analysis (RDA). cDNA fragments were subjected to three rounds of PCR-coupled subtractive hybridization and the final difference products were cloned into the BamHI restriction site of the pBluescript SK+ phagemid vector and transformed into XL1 MRF- chemically competent host cells (Stratagene). Transcripts were purified from the host cells by Rolling Circle Amplification (GE Healthcare) and sequenced by the DNAcore facility at the Hubbard Center for Genome Studies, University of New Hampshire. Sequences represent genes that were differentially expressed between the two strains. The195SR library contains transcripts that were upregulated in SR aortic tissue relative to the corresponding site in the WC aorta. The corresponding WC library (195WC) contains transcripts that were upregulated in the WC aortic tissue relative to 195SR.

characteristic: description

text: XL1 MRF-

text: Columba livia

characteristic: organism

text: BAMHI

text: Mixed

text: Show Racer

text: Descending Aorta

text: pBluescript SK+


characteristic: External Id

text: University of New Hampshire

text: live

text: Generic

text: Mixed

text: The descending aorta from Six-week old atherosclerosis-resistant Show Racer (SR) pigeons and atherosclerosis-susceptible (spontaneous, non-induced) White Carneau (WC) pigeons were used to determine differences in genetic expression between the two breeds. Total RNA was extracted (Trizol, Invitrogen), purified, and converted to double-stranded cDNA using the BD SMART PCR cDNA Synthesis Kit (Clontech). Full-length cDNA from each pigeon strain was cut with DpnII (5'GATC) in order to prepare amplicons for comparison by Representational Difference Analysis (RDA). cDNA fragments were subjected to three rounds of PCR-coupled subtractive hybridization and the final difference products were cloned into the BamHI restriction site of the pBluescript SK+ phagemid vector and transformed into XL1 MRF- chemically competent host cells (Stratagene). Transcripts were purified from the host cells by Rolling Circle Amplification (GE Healthcare) and sequenced by the DNAcore facility at the Hubbard Center for Genome Studies, University of New Hampshire. Sequences represent genes that were differentially expressed between the two strains. The195WC library contains transcripts that were upregulated in WC aortic tissue relative to the corresponding site in the SR aorta. The corresponding SR library (195SR) contains transcripts that were upregulated in the SR aortic tissue relative to WC.

text: XL1 MRF-

text: Columba livia

text: BAMHI

text: Mixed

text: White Carneau

text: Descending Aorta

characteristic: title

text: pBluescript SK+


text: Mount Desert Island Biological Laboratory

text: live

text: Generic

text: melanoma derived

text: XM cell line

characteristic: cell-line

text: The XM cell line was derived from from skin and fin of two tumor-bearing Xiphophorus BC1 hybrids (Xiphophorus maculatus x Xiphophorus helleri x Xiphophorus helleri; Xiphophorus Genetic Stock Center, San Marcos, TX). The Hyb BBC1 helleri x X. maculatus was strain H005BC1, Pedigree 8921, date of birth 9/16/03. The X. maculatus used were Strain 163A, virgin females from flock date of birth 12/19/03. Cells were grown in culture as described by David Barnes, Lori Dowell, David Forest, Angela Parton, Peter Pavicevic, and Steven Kazianis. 2006. Characterization of XM, a Novel Xiphophorus Melanoma-Derived Cell Line. Zebrafish 3(3): 371-381. Using TRIzol reagent, total RNA was isolated from 0.5 x10e8 cells. The primary cDNA library was constructed from mRNA and directionally cloned into pCMV-SPORT6.1. The host bacterium was DH10B T1 (T1 and T5 phage resistant). Standard cDNA construction and quality control were performed by Invitrogen. The average insert size was 1.7 kb and the unamplified titer was 1.27 x 10e6 cfu/ml.

text: two adults

characteristic: dev-stage

text: E. coli

text: Xiphophorus maculatus x Xiphophorus hellerii

text: NotI

text: EcoRV

text: melanoma

text: Plasmid

text: pCMVSport6_1 with ccdB verA


text: Soochow University

characteristic: INSDC center name

text: live

text: Generic

text: hemocytes

text: healthy and unmoulted giant freshwater prawns

text: Macrobrachium rosenbergii

text: hemolymph

text: LPS-treated 1 hr Library


text: Soochow University

text: live

text: Generic

text: hemocytes

text: Macrobrachium rosenbergii

text: hemolymph

characteristic: tissue-type

text: LPS-treated 12 hr Library


text: National Institute of Agricultural Science and Technology, RDA

text: live

text: Generic

text: Embryonic hour 18 post oviposition

text: Bombyx mori

text: Embryo

text: Bombyx mori early embryonic full-length cDNA library


text: National Institute of Agricultural Biotechnology

text: live

text: Generic

text: Yeoung San

characteristic: cultivar

text: The leaf tissue from the 3-4 leaf stage was used to prepare RNA (driver) and embryo 10 DAF was used to prepare RNA (tester). Suppression subtractive hybridization was performed according to the manufacturer's instructions using the polymerase chain reaction (PCR)-based cDNA subtraction kit (Clontech, Mountain View, CA, USA). The PCR products were ligated into the pCR2.1-TOPO vector (Invitrogen, Carlsbad, CA, USA) to generate the subtracted cDNA library.

text: 3-4 leaf stage and embryo 10 DAF (days after flowering)

text: E.coli

text: Brassica napus

text: EcoRI

text: Leaf and Seed

text: Suppression subtractive hybridization cDNA library from embryo 10 DAF (Ref. leaf)

text: pCR 2.1-TOPO

characteristic: vector


characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Yeoung San

text: The embryo 10 DAF was used to prepare RNA (driver) and embryo 35 DAF was used to prepare RNA (tester). Suppression subtractive hybridization was performed according to the manufacturer's instructions using the polymerase chain reaction (PCR)-based cDNA subtraction kit (Clontech, Mountain View, CA, USA). The PCR products were ligated into the pCR2.1-TOPO vector (Invitrogen, Carlsbad, CA, USA) to generate the subtracted cDNA library.

text: Embryo 10 and 35 DAF (days after flowering)

text: E.coli

text: Brassica napus

text: EcoRI

text: Immature seed

text: Suppression subtractive hybridization cDNA library from embryo 35 DAF (Ref.embryo 10 DAF)

text: pCR 2.1-TOPO


text: live

characteristic: INSDC status

text: Generic

text: Yeoung San

text: The embryo 35 DAF was used to prepare RNA (driver) and embryo 10 DAF was used to prepare RNA (tester). Suppression subtractive hybridization was performed according to the manufacturer's instructions using the polymerase chain reaction (PCR)-based cDNA subtraction kit (Clontech, Mountain View, CA, USA). The PCR products were ligated into the pCR2.1-TOPO vector (Invitrogen, Carlsbad, CA, USA) to generate the subtracted cDNA library.

text: E.coli

characteristic: lab-host

text: Brassica napus

text: EcoRI

text: Immature seed

text: Suppression subtractive hybridization cDNA library from embryo 10 DAF (Ref. embryo 35 DAF)

text: pCR 2.1-TOPO


text: live

text: Generic

characteristic: NCBI submission model

text: Yeoung San

text: The embryo 10 DAF was used to prepare RNA (driver) and embryo 25 DAF was used to prepare RNA (tester). Suppression subtractive hybridization was performed according to the manufacturer's instructions using the polymerase chain reaction (PCR)-based cDNA subtraction kit (Clontech, Mountain View, CA, USA). The PCR products were ligated into the pCR2.1-TOPO vector (Invitrogen, Carlsbad, CA, USA) to generate the subtracted cDNA library.

text: Embryo 10 and 25 DAF (days after flowering)

text: E.coli

text: Brassica napus

text: EcoRI

text: Immature seed

text: Suppression subtractive hybridization cDNA library from embryo 25 DAF (Ref. embryo 10 DAF)

text: pCR 2.1-TOPO


text: University of Louisville

text: live

text: Generic

characteristic: NCBI submission package

text: Constructed from RNA extracted from 10 adult male glands with the ZAP Express XR Library Construction Kit (Stratagene)

text: Plethodon shermani

text: Male

characteristic: sex

text: Mental gland

text: Plethodon shermani male mental gland library

text: phagemid

text: Lambda-ZAP vector


text: Instituto de Biociencias de Botucatu - IBB/UNESP

text: live

text: Generic

text: Creator(tm) SMART(tm) cDNA Library Construction kit (BD Biosciences)

characteristic: description

text: adult

text: midgut

characteristic: organ

text: Anopheles aquasalis

text: Sfi IA

text: Sfi IB

text: female

text: midgut cDNA library from female Anopheles aquasalis

text: plasmid

text: pDNR-LIB


characteristic: External Id

text: Dartmouth Medical School

text: live

text: Generic

text: CHO, Wild Type

text: Total mRNA was isolated from WT CHO cells using oligo dT beads and reverse-transcribed to form a cDNA library.

text: Cricetulus griseus

characteristic: organism

text: Wild Type Chinese Hamster Ovary Cell Line


text: USDA-ARS

text: live

text: Generic

text: Subtraction Suppression Hybridization library constructed using Clontech PCR-Select cDNA subtraction kit. Hydrated gametophytic Poly A RNA used to construct the driver cDNA, Polysomal Poly A plus from slow dried and rapid-dried rehydrated gemetophytes used to construct the target cDNAs. pUC19r is a modified pUC19 plasmid containing a polylinker insert that when cut with XcmI generate T/A overhangs and flanking BamHI sites (Velten, J., and Oliver, M.J. (2001) Plant Mol. Biol. 45:713-722).

text: Gametophytic

text: Gametophyte

text: Syntrichia ruralis

text: BamHI

text: BamHI

text: Leaf (dried And rehydrated)

text: Tortula ruralis SSH

characteristic: title

text: Plasmid


text: USDA-ARS

text: live

text: Generic

text: Source: Greehouse-reared colony. A high quality EST with at least 150 contiguous bases at KB basecaller score of 20 or better. Library construction by Christine A. Lynch (USDA-ARS-USHRL).

text: Adult

characteristic: dev-stage

text: Diaphorina citri

text: EcoRI

text: XhoI

text: Mixed

text: midgut

text: Asian citrus psyllid midgut (Hemiptera: Psyllidae)

text: pBluescript II SK+


text: University of California, Los Angeles

characteristic: INSDC center name

text: live

text: Generic

text: Asgrow A3237

text: Soybean were grown in the UCLA Plant Growth Center with a 16:8 light-dark cycle at 22oC. Total RNA isolated from soybean seeds containing globular-stage embryos was subjected two rounds of poly(A) selection using a Dynabeads oligo(dT) system (Invitrogen). Complementary DNA (cDNA) was generated from 500 ng of poly(A)-selected mRNA using a SMART PCR cDNA Synthesis kit (Clontech) with 20 cycles of PCR amplification. The cDNA sample was digested with a restriction enzyme Sfi I (New England Biolabs) and then size-fractionated over the Chroma-spin column (Clontech). Fractions containing Sfi I-digested cDNA with size greater than 400 bp were pooled and precipitated. Five micrograms of the size-selected Sfi I-digested cDNA sample was sent to Roche 454 Sequencing Core Facility for generating Soybean Globular-Stage Seed 454 ESTs.

text: Globular-stage

text: Whole seeds

text: Glycine max

text: Soybean Seeds Containing Globular-Stage Embryos


text: Consorci CSIC-IRTA

text: live

text: Generic

text: 30 days after pollination

text: Zea mays

text: scutellum

characteristic: tissue-type

text: Zea mays scutellum 30 days after pollination


text: National Institute of Radiological Sciences

text: live

text: Generic

text: fibroblast

characteristic: cell type

text: HFLIII

characteristic: cell-line

text: differential display

text: 11 weeks

text: Homo sapiens

text: embryo

text: Homo sapiens HFLIII 11 weeks embryo


text: Chung-Ang University

characteristic: INSDC first public

text: live

text: Generic

text: coelomic cell

text: Eisenia andrei

text: EcoRI

text: XhoI

text: Eisenia andrei coelomic cell cDNA library

text: pCMV-Script XR

characteristic: vector


text: National Institute of Agrobiological Sciences

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: hemocyte

text: Spinning stage day=0

text: Bombyx mori

text: female/male mixed

characteristic: strain

text: Bombyx mori C108 spinning stage day=0


text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: 4th-instar larva day-2

text: Bombyx mori

text: mixed

text: midgut


text: live

text: Generic

text: 5th-instar larva

text: Bombyx mori

text: mixed

characteristic: sex

text: microbe-infected fat body

text: MFB-


text: live

text: Generic

text: 12-40hr after oviposition

text: Bombyx mori

text: mixed

text: diapause-destined embryo

text: dpe-


text: live

text: Generic

text: mixture of 5th-instar larva, spinning stage

text: Bombyx mori

text: mixed

text: wing disc

text: wd--


text: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences

text: live

text: Generic

text: Sus scrofa

text: Sus scrofa preimplantation embryos


text: National Taiwan University

text: live

text: Generic

text: Aedes aegypti

text: whole animals

text: Subtracted cDNA library between permethrin resistant and susceptible strain


text: University of Messina

text: live

text: Generic

text: Liver

characteristic: organ

text: Coris julis

text: Coris julis liver cDNA RT-PCR


characteristic: External Id

text: University of Florida

text: live

text: Generic

text: EST library

characteristic: description

text: Pleurobrachia bachei

text: total animal

text: EST library of the total animal Pleurobrachia bachei


text: Brigham Young University

text: live

text: Generic

text: Clonetech PCR Selection cDNA Subtraction Kit

text: Mixed

text: Plectus murrayi

characteristic: organism

text: Whole nematode

text: Plectus murrayi desiccated subtractive library

characteristic: title


text: The University of Tokyo

characteristic: INSDC center name

text: live

text: Generic

text: adult

characteristic: dev-stage

text: Lethenteron camtschaticum

text: liver

text: Lethenteron camtschaticum liver adult


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 0.5-2kb and 2-8kb using 1.1% agarose gel electrophoresis, then ligated into NotI and SalI-digested pCMV-SPORT6 vector, pooled and transformed. The library was constructed and sequencing performed at the DOE Joint Genome Institute (Walnut Creek, CA).

characteristic: lab-host

text: Bipolaris maydis

text: SalI

text: NotI

text: CCBO Cochliobolus heterostrophus, Chet minimal


text: DOE Joint Genome Institute

text: live

text: Generic

text: Bipolaris maydis

text: SalI

text: NotI

text: CCBP Cochliobolus heterostrophus, Chet complete


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT)-primed cDNA and cloned into vector pDNR-LIB (Clontech Laboratories, Inc., Mountain View, CA) using the SMART system (BD Biosciences, San Jose, CA). To generate the first strand, polyA RNA was primed with an oligo(dT) primer (5'-ATTCTAGAGGCCGAGGCGGCCGACATG(T)30 VN-3', where V = A, G, or C). The second strand was synthesized by PCR using primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' followed by digestion of the SfiI sites. This library was normalized using mRNA as starting material. Library was created and sequencing done by the DOE Joint Genome Institute (Walnut Creek, CA).

text: Bipolaris maydis

text: SfiIA

text: SfiIB

text: CCBU Cochliobolus heterostrophus, Chet complete, normalized

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT)-primed cDNA and cloned into vector pDNR-LIB (Clontech Laboratories, Inc., Mountain View, CA) using the SMART system (BD Biosciences, San Jose, CA). To generate the first strand, polyA RNA was primed with an oligo(dT) primer (5'-ATTCTAGAGGCCGAGGCGGCCGACATG(T)30 VN-3', where V = A, G, or C). The second strand was synthesized by PCR using primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' followed by digestion of the SfiI sites. This library was normalized using total RNA as starting material. Library was created and sequencing done by the DOE Joint Genome Institute (Walnut Creek, CA).

text: Bipolaris maydis

text: SfiIA

text: SfiIB

text: CCBW Cochliobolus heterostrophus, Chet complete, normalized

text: pDNR-LIB


text: University of Louisville

text: live

text: Generic

text: Constructed from RNA extracted from 20 adult male glands with Creator Smart cDNA Library Construction Kit (BD Biosciences Clonetech)

text: Desmognathus ocoee

text: Male

text: Mental gland

characteristic: tissue-type

text: Desmognathus ocoee male mental gland library

text: Plasmid

text: pDNR-LIB


text: Biology Centre ASCR

characteristic: INSDC first public

text: live

text: Generic

text: root

text: Medicago truncatula

text: Ogre MT1 intron library


text: Biology Centre ASCR

text: live

text: Generic

text: root

text: Medicago truncatula

text: Ogre MT2 intron library


text: Biology Centre ASCR

text: live

text: Generic

text: root

text: Medicago truncatula

text: Ogre MT3 intron library


text: Biology Centre ASCR

text: live

text: Generic

text: root

text: Medicago truncatula

text: Ogre MT4 intron library


text: Ministry of Agriculture

text: live

text: Generic

text: Tripidium arundinaceum

text: Saccharum arundinaceum Retz SSH cDNA library

text: pMD 18-T

characteristic: vector


text: MPI for Molecular Genetics

characteristic: INSDC last update

text: live

text: Generic

text: collection_date: 10-Oct-2004; collected_by: Juan Fuentes and Carla Viegas; isolation_source: aquaculture fish. Total RNA was extracted from 9 specimens of both sex (4 females and 5 males) and weight range 350 g to 4kg. Collected tissue frozen in liq N2 and stored at -80C prior to RNA extraction. mRNA purification and concentration were performed using the Dynal's oligo-dT magnetic beads. Library construction was performed with Creator SMART cDNA library construction kit (Clontech) and the thermostable duplex-specific nuclease (Zhu et al., 2001). Initial LD PCR amplification of 22 cycles for enrichment of full length transcripts was verified on Agarose gels. cDNA sizes (>500 base pairs) were selected using filter columns based on agarose gels as described in the Clontech manual. Library contruction and sequencing were performed at the MPI of Molecular Genetics Berlin.

text: adult

text: Sparus aurata

text: mixed

text: ultimobranchial gland tissue


text: MPI for Molecular Genetics

text: live

characteristic: INSDC status

text: Generic

text: adult

text: Sparus aurata

text: mixed

text: corpuscles of stannius


text: UMR 7138 SAE, Universite Pierre Et Marie Curie

text: live

text: Generic

text: country: France:La Ciotat

text: adult

text: Oopsacas minuta

text: whole organism

text: Oopsacas minuta whole organism adult


text: live

text: Generic

text: country: France:Marseille

text: adult

text: Oscarella lobularis

text: whole organism

text: Oscarella lobularis whole organism adult


text: live

text: Generic

text: country: France:Marseille

text: adult

text: Sycon raphanus

text: whole organism

text: Sycon raphanus whole organism adult


text: live

text: Generic

text: Clytia hemisphaerica

text: whole organism

text: Clytia hemisphaerica whole organism


text: live

text: Generic

text: country: Belgium; collection_date: 2003.

text: young sponge after gemmulation

text: Ephydatia muelleri

text: whole organism

text: Ephydatia muelleri whole organism young sponge after gemmulation


text: Bodoe University College, Norway

text: live

text: Generic

text: SSH library using RNA from fertilized eggs as tester and from 10-somite stage embryos as driver

text: Embryonic

text: E.coli

text: Hippoglossus hippoglossus

text: Subtracted maternal mRNA library of Atlantic halibut

text: Plasmid


text: National Institute of Allergy and Infectious Diseases

text: live

text: Generic

text: unidirectional library was constructed using long distance PCR (Smart cDNA kit) and Lambda TriplEx2 vector

text: adult female

text: salivary glands

text: Anopheles darlingi

text: female

text: Adult female salivary gland cDNA library Adarg2

text: Phage

text: Lambda TriplEx2


text: live

text: Generic

text: adult female

text: salivary glands

text: Simulium vittatum

text: female

text: Adult female salivary gland cDNA library S_vit1

text: Phage

text: Lambda TriplEx2


text: Auburn University

text: live

text: Generic

text: C.colocynthis seedlings at 4-6 leaf stage were treated by 20% PEG (8000). cDNA-AFLP was performed to isolate genes from the roots.

text: Seedling 4-6 leaf stage

text: Roots

text: Citrullus colocynthis

text: Citrullus colocynthis drought stress with cDNA-AFLP


text: Center of Marine Biotechnology

text: live

text: Generic

text: A random hexamer primed cDNA library in Lambda Zap II was prepared from P. furiosus total RNA.

text: E. coli XL1-blue

text: Pyrococcus furiosus DSM 3638

text: Eco RI

text: DSM 3638

characteristic: strain

text: Pyrococcus furiosus, cDNA

text: Phage

text: Lambda Zap II


text: Michigan State University

text: live

text: Generic

characteristic: NCBI submission model

text: Canis lupus familiaris

text: Dog cDNA


text: Amersham Life Science, Inc.

text: live

text: Generic

characteristic: NCBI submission package

text: Genomic DNA was purified from P. furiosus cells and partially digested with Dra I, Eco RV, Hinc II, Pvu II, size selected, ligated to Eco RI linkers then cloned into the Eco RI site of l-ZAP II, plasmid excision vector. Excision was performed in batch and individual clones retrieved by plating.

text: E. coli

text: Pyrococcus furiosus DSM 3638

text: Eco RI

text: Eco RI

text: DSM 3638

text: Pyrococcus furiosus l-ZAP II library, F Robb

text: l/plasmid excision vector

text: l-ZAP II


text: Texas A&M University

text: live

text: Generic

text: Cenchrus ciliaris

text: obligatory sexual ovary

text: Buffelgrass obligatory sexual ovary (M.A.Hussey)


text: Texas A&M University

text: live

text: Generic

text: Cenchrus ciliaris

text: obligatory apomictic ovary

text: Buffelgrass obligatory apomictic ovary (M.A.Hussey)


text: Universitaets-Krankenhaus Eppendorf

text: live

text: Generic

text: Canis lupus familiaris

text: cerebrum (occipital lobe)

text: Dog occipital lobe ESTs


text: University of Queensland

text: live

text: Generic

text: larvae

text: Rhipicephalus microplus

text: Boophilus microplus larvae

text: plasmid

text: pBluescript


text: University of Cambridge

text: live

text: Generic

text: adult

text: Canis lupus familiaris

text: retina

text: Dog retina cDNA


text: Southern Illinois University

text: live

text: Generic

text: mRNA derived from the polysomal mRNP fraction of desiccated Tortula ruralis gametophytes, directionally cloned cDNA (lambda ZAPII) and in vivo excised to pBluescript (Stratagene)

text: Syntrichia ruralis

text: EcoRI

text: XhoI

text: Desiccated Gametophytes

text: mRNP Lambda ZapII Express Library

text: Plasmid

text: pBluescriptII


text: The University of Tokyo

text: live

text: Generic

text: mRNA arbitrarily primed RT-PCR (RAP RT-PCR) product; primer A5 from Stratagene RAP-PCR kit

text: Chaetoceros compressus

text: Chaetoceros compressum library (Watabe S)


text: Institute for Molecular and Cellular Regulation, Gunma University

text: live

text: Generic

text: Canis lupus familiaris

text: cerebral artery

text: Canis familiaris cerebral artery library


text: Institute of Molecular and Cell Biology

text: live

text: Generic

text: Bacilli were disrupted using a Mini bead beater (Biospec). RNA was isolated using the RNeasy protocol (Qiagen). Purified RNA was subjected to 2 rounds of digestion with RNase-free DNase I (Promega). DNase I was heat-inactivated at 75oC for 5 min. and removed by using RNeasy columns followed by phenol extraction and ethanol precipitation. The RNA preparations were confirmed to be free of genomic DNA contamination by carrying out PCR and RT-PCR using the Access kit (Promega) and primers specific for the histone-like protein gene hlp (Lee et al., 1998). cDNA was synthesized using random hexamer primers (Promega) and Stratagene's cDNA synthesis kit. cDNA fragments were ligated into lambda ZAP II vector and packaged in vitro using Stratagene reagents.

text: E. coli XL1-Blue MRF'

text: Mycolicibacterium smegmatis MC2 155

text: Mycobacterium anaerobic stationary phase library

text: Lambda ZAP II


text: Wonju College of Medicine, Yonsei University

text: live

text: Generic

text: country=South Korea: Kangwon Province

text: Vespertilio sinensis

text: Vespertilio superans library


text: University of Maryland

text: live

text: Generic

text: 717 1B4

characteristic: cultivar

text: Populus tremula x Populus alba

text: bark

text: Populus alba x Populus tremula bark


text: University of Maryland

text: live

text: Generic

text: 717 1B4

text: cDNA synthesized from from poplar bark treated with low-temperatures

text: Low temperature treated

text: Populus tremula x Populus alba

text: Bark

text: Poplar bark first strand cDNA

text: Plasmid


text: Tokyo University of Technology

text: live

text: Generic

text: Plants were treated with 0.5 M NaCl for a month. Differentially expressed cDNA fragment was obtained from differential display analysis, and was subcloned into pGEM-T vector.

text: Bruguiera gymnorhiza

text: leaf treated with 0.5 M NaCl

text: Bruguiera gymnorhiza leaf treated with 0.5 M NaCl


text: University of Kentucky

text: live

text: Generic

text: P. carinii organisms (3x10e9) from a single rat (99-1-6, sacrificed on 3/17/99) at Cincinnati VA facilities. Trizol extracted RNA. Oligo dT priming, standard conditions described by vendor, Stratagene. Further details see www.uky.edu/Project/Pneumocystis/

text: E. coli

text: Pneumocystis carinii

text: EcoRI

text: XhoI

text: phage/plasmid

text: Lambda ZAP II


text: live

text: Generic

text: Vespertilio sinensis

text: liver

text: Vespertilio superans liver


text: Animal Health Trust

text: live

text: Generic

text: Canis lupus familiaris

text: liver

text: Canis familiaris liver


text: Gunma University, Institute for Molecular and Cellular Regulation

text: live

text: Generic

text: Canis lupus familiaris

text: cerebral artery

text: dog cerebral artery cDNA library


text: Cold Spring Harbor Laboratory

text: live

text: Generic

text: The library was provided by Greg Hannon (Cold Spring Harbor Laboratory). This library is oligo(dT) primed using stratagene zap cDNA synthesis kit. It was made from exponentially growing mdck cells. Please contact Greg Hannon (hannon@cshl.org) with any library related inquiries.

text: Canis lupus familiaris

text: Canis cDNAs from mdck cells

text: Lambda Zap II


text: Korea Univ.

text: live

text: Generic

text: Phalaenopsis japonica

text: Aerides japonica mRNA


text: The Biology Department of Shandong Normal University

text: live

text: Generic

text: total RNA extraction from NaCl(400mM) treated Suaeda salsa by RNAgent kit(Promega); mRNA isolation by MESSAGEMAKER kit(GIBCO BRL); directional cDNA synthesis(EcoRI XhoI) by cDNA synthesis kit(STRATAGEN); the ZAP express library by GigapackIII Gold Cloning kit(STRATAGENE)

text: seedling

text: aerial part tissue

characteristic: organ

text: Suaeda salsa

text: EcoRI

text: XhoI

text: Suaeda salsa ZAP cDNA library

text: Phage

text: lambda zap


characteristic: External Id

text: Murdoch University

text: live

text: Generic

text: A mixture of mycelial cultures grown in liquid B5 for 48 hours and transferred for 24 hours to media lacking carbon, nitrogen or supplemented with hydrogen peroxide

text: Fulvia fulva

text: Race 4

text: Mycelium

text: In vitro expressed cDNAs

text: Lambda bluescript


text: Cold Spring Harbor Laboratory

text: live

text: Generic

text: The library was produced by Greg Hannon and Raymond Preston (Cold Spring Harbor Laboratory). This library is oligo(dT) primed using stratagene zap cDNA synthesis kit. It was made from dog testes. Please contact Greg Hannon (hannon@cshl.org) with any library related inquiries.

characteristic: description

text: Canis lupus familiaris

text: testes

text: Canis cDNAs from testes cells

text: Lambda Zap II


text: University of Cambridge

text: live

text: Generic

text: Canis lupus familiaris

characteristic: organism

text: CR, retinal


text: University of Cambridge

text: live

text: Generic

text: Canine EST from subtracted retinal library SL

text: Canis lupus familiaris

text: retinal library CFSR

characteristic: title


text: University of Peking Unviversity Life Sciences Center

characteristic: INSDC center name

text: live

text: Generic

text: Drought-induced EST from Boea Crassifolia via mRNA DD-PCR

text: Paraboea crassifolia

text: Drought-induced EST from Boea crassifolia


text: John Innes Centre

text: live

text: Generic

text: mesophyll cells

characteristic: cell type

text: 24 hours induced by auxin and cytokinin

characteristic: dev-stage

text: Zinnia elegans

text: Zinnia elegans 24 hours induced by auxin and cytokinin


text: John Innes Centre

text: live

text: Generic

text: mesophyll cells

text: 30 min induced by auxin and cytokinin

text: Zinnia elegans

text: Zinnia elegans 30 min induced by auxin and cytokinin


text: John Innes Centre

text: live

text: Generic

text: mesophyll cells

text: 48 hours induced by auxin and cytokinin

text: Zinnia elegans

text: Zinnia elegans 48 hours induced by auxin and cytokinin


text: University of Minnesota

text: live

text: Generic

text: Tissue was taken from the frontal, occipital, temporal and parietal lobes, olfactory bulb, hippocampus, cerebellum, thalamus, hypothalamus, midbrain, pons, and medulla.

text: Brain

text: Canis lupus familiaris

text: NotI

text: SalI

text: Male

characteristic: sex

text: Canine Brain cDNA Library

text: Plasmid

text: pSPORT1 (Gibco BRL)


text: Dept. of Forest Mycology & Pathology

characteristic: INSDC first public

text: live

text: Generic

text: The subtractive hybridization cDNA library was constructed from scots pine roots infected for 6-days with mycelia of Heterobasidon annosum (FP5).

text: Seedling roots of scots pine were infected for 6 days with H. annosum

text: Pinus sylvestris/Heterobasidion annosum mixed EST library

text: EcoRI

text: Heterobasidion annosum - Scots pine infection stage subtraction cDNA library (hasp)

text: Plasmid

text: pT-Adv


text: University of Maryland

text: live

text: Generic

text: 717 1B4

text: cDNA synthesized from poplar developing reaction wood of stems treated by mechanical bending after 1, 2, 4, 8, 16 or 32 days

text: 1, 2, 4, 8, 16 or 32 days after mechanical stress

text: Populus tremula x Populus alba

text: stem reaction wood

characteristic: tissue-type

text: Poplar developing reaction wood first strand cDNA

text: Plasmid

text: pCRII-TOPO

characteristic: vector


text: Miyazaki University, Faculty of Agriculture

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: reproductive cell

text: isolate 1

text: embryo sac formation

text: Megathyrsus maximus

text: apospory

text: young bud

text: Panicum maximum young bud embryo sac formation


text: University of Georgia, Center for Applied Genetic Technologies

text: live

text: Generic

characteristic: NCBI submission model

text: Cenchrus ciliaris

text: Apomictic pistil cDNA library

text: Plasmid


text: Menzies School of Health Research

text: live

text: Generic

characteristic: NCBI submission package

text: Randomly primed cDNA library in pGEX 4T-2 from approximately 100 human S. scabiei mites

text: Mixed stage

text: Sarcoptes scabiei

text: EcoRI

text: SmaI

text: var. hominis

characteristic: strain

text: Scabies pGEX 4T-2 random primed library

text: plasmid

text: pGEX 4T-2


text: Menzies School of Health Research

text: live

text: Generic

text: Oligo-dT primed cDNA library in lambda zap express from approximately 600 human S. scabiei mites

text: Mixed stage

text: Sarcoptes scabiei

text: EcoRI

text: XhoI

text: var. hominis

text: Scabies lambda zap express cDNA library

text: phage

text: lambda zap express


text: San Francisco State University

text: live

text: Generic

text: non-differentiated

text: cadmium tolerant

characteristic: cell-line

text: Datura inoxia

text: Plant cell culture

text: Cd2+-induced pT-Adv


characteristic: External Id

text: Indian Institute of Horticultural Research

text: live

text: Generic

text: It is a cDNA product obtained by RT-PCR using chitinase gene specific primers

text: Sporulating stage

text: Trichoderma lixii

text: Xba I

text: Bam HI

text: Mycelium

text: Trichoderma harzianum fungus cDNA

text: Plasmid

text: PUC 57


text: University Clinic, Heidelberg

text: live

text: Generic

text: heart

characteristic: organ

text: Canis lupus familiaris

text: RDA preconditioning library

text: Plasmid

text: pGEM-T Easy


text: Cold Spring Harbor Laboratory

text: live

text: Generic

text: The library was provided by Greg Hannon and Lee Santos (Cold Spring Harbor Laboratory). This library is oligo(dT) primed using stratagene zap cDNA synthesis kit. It was made from dog whole brain cells. Please contact Greg Hannon (hannon@cshl.org) with any library related inquiries.

characteristic: description

text: Canis lupus familiaris

characteristic: organism

text: Canis Total Brain cDNAs

text: Lambda Zap II


text: The Natural History Museum

characteristic: INSDC center name

text: live

text: Generic

text: Platystomos albinus

text: Platystomus albinus cDNA

characteristic: title

text: pCRII


text: University of Georgia

text: live

text: Generic

characteristic: lab-host

text: Cenchrus ciliaris

text: apomictic pistil

text: pPAP

text: Plasmid

text: pBluescript


text: John Innes Centre

text: live

text: Generic

text: mesophyll cells

characteristic: cell type

text: Zinnia elegans

text: Zinnia elegans mesophyll cells


text: John Innes Centre

characteristic: INSDC first public

text: live

text: Generic

text: mesophyll cells

characteristic: dev-stage

text: Zinnia elegans

text: Zinnia elegans induced within 4h after auxin and cytokinin addition


text: Korea Univ.

text: live

text: Generic

text: expression identified by suppression subtractive hybridization using common type and alba type flowers

text: Phalaenopsis japonica

text: flower

characteristic: tissue-type

text: Aerides japonica flower


text: Dept. of Forest Mycology & Pathology

characteristic: INSDC last update

text: live

text: Generic

text: The subtractive hybridization cDNA library was constructed from scots pine roots infected for 6-days with mycelia of Heterobasidon annosum (FP5).

text: EcoRI

text: Heterobasidion annosum - Scots pine infection stage (HAGE) subtraction cDNA library

text: Plasmid

text: pT-Adv

characteristic: vector


text: University of Edinburgh

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: The library was prepared by Eva Jimenez, University of Barcelona, Spain The sequencing was performed by Eva Jimenez, Aziz Aboobaker and Ailie Rosie, Edinburgh Phylogenomics Programme, ICAPB, Edinburgh, UK.

text: embryo

text: Symsagittifera roscoffensis

text: EcoRI

text: HinDIII

text: mixed

characteristic: sex

text: Convoluta roscoffensis embryos from Eva Jiminez

text: phage

text: lambda triplex


text: Washington University School of Medicine

text: live

text: Generic

characteristic: NCBI submission package

text: PCR products were directly cloned into TA cloning vector (pre-cut vector pCR2.1-TOPO was purchased from Invitrogen) Average insert size is 300bp. Our Insert also have same adaptor sequence on both ends. The sequence of the adaptor is: 5'-ATACGTGCAGGCTGGTTACATCTG-3'.

text: Canis lupus familiaris

text: Brain from a dog with encephalitis

text: SV DOGRDA RD3

text: plasmid (ampicillin resistant)


characteristic: External Id

text: Vanderbilt University

text: live

text: Generic

text: heart

text: Tissue source: dog heart (adult, 30 day - 40 day fetal), right and left atria and ventricle. Dog breed - mixed (beagle, German shepherd, pointer, Irish setter). Library construction: oligo-dT primed

text: mixed developmental stages (adult, 30 day - 40 day fetal)

text: heart

text: Canis lupus familiaris

text: 5' of vector NotI

text: 3' of vector EcoRI

text: heart

text: Canine heart non-normalized cDNA Library in pBluescript

text: Plasmid

text: pBluescript


text: Vanderbilt University

text: live

text: Generic

text: heart

text: heart

characteristic: organ

text: Canis lupus familiaris

text: 5' of vector NotI

text: 3' of vector EcoRI

text: heart

text: Canine heart normalized cDNA Library in pBluescript

text: Plasmid

text: pBluescript


text: RIKEN Plant Science Center

text: live

text: Generic

text: Canary bird

characteristic: cultivar

text: cultured in tracheary element differentiation-inductive medium

characteristic: description

text: Zinnia elegans

text: mesophyll cell

text: zinnia cultured mesophyll cell equalized cDNA

text: pGEM-T easy


text: E. I. DuPont de Nemours and Company

characteristic: INSDC center name

text: live

text: Generic

text: monococcum

text: Wheat (Triticum aestivum, monococcum) developing seedling

text: Triticum monococcum

characteristic: organism

text: EcoRI

text: XhoI

text: seedling

text: pBluescript SK+


text: West Virginia University

text: live

text: Generic

text: The library was generated by a subtraction of tall fescue-N. coenophialum symbiotum cDNA (tester) with cDNA from uninfected tall fescue supplemented with saprophytically grown N. coenophialum (driver) by SSH. Experimental evidence suggests that the ESTs are likely to be plant in origin except for clone Nc25.

text: Mature

text: Schedonorus arundinaceus/Neotyphodium coenophialum mixed EST library

text: Pseudostem

text: Neotyphodium coenophialum-tall fescue subtracted SSH library

characteristic: title

text: Plasmid

text: p-bluescript


text: West Virginia University

text: live

text: Generic

text: The library was generated by a subtraction of uninfected tall fescue cDNA (tester) with tall fescue-N. coenophialum symbiotum cDNA (driver) by SSH.

text: Mature

text: Lolium arundinaceum

text: Pseudostem

text: tall fescue subtracted SSH library

text: Plasmid

text: p-bluescript


text: West Virginia University

text: live

text: Generic

text: The lambda ZAP II cDNA (Stratagene) library was prepared from tall fescue infected with Neotyphodium coenophialum (ATCC 90664). Experimental evidence suggests that the ESTs are likely to be plant in origin except for clone ZAPNc25.

text: Mature

text: Pseudostem

text: Neotyphodium coenophialum-tall fescue lambda ZAP II cDNA library


text: UMR INRA/Universite Henri Poincare 1136

text: live

text: Generic

text: clone 'INRA 717-1-B4'

text: cDNA library of three-week-old roots from Populus tremula L. x P.alba L. (clone 'INRA 717-1-B4') incubated in 5uM indole-3-acetic acid 24h before harvesting. The cDNA library was constructed from 1 ug of total RNA using the SMART cDNA synthesis kit (Clontech, Palo Alto, CA, USA) according to the manufacturer's instructions. The resulting cDNA was packed into lambda phages using the Gigapack III Gold packaging kit (Stratagene, La Jolla, CA). The pTriplEx2 phagemid clones in Escherichia choli were obtained by using the mass in vivo excision protocol according to the manufacturer's instructions (Clontech).

text: three-week-old

text: root

text: Populus tremula x Populus alba

text: three-week-old roots incubated in 5uM indole-3-acetic acid 24h before harvesting