text: The Samuel Roberts Noble Foundation

text: live

text: Generic

text: Medicago truncatula leaves were infected with alfalfa mosaic virus (AMV). AMV infected leaves were harvested at 14 & 17dpi. Equal amounts of total RNA were isolated and pooled for mRNA purification. RNA for cDNA library construction was isolated from the pooled tissues. The cDNA was directionally ligated into the Uni-Zap XR vector (Stratagene) and packaged using the Gigapack III Gold packaging extracts. Phagemids containing cDNA inserts were in vivo excised from the recombinant Uni-ZAP XR vector using ExAssist helper phage and the E. coli strain XL1-Blue MRF' (Stratagene). Excised plasmids were plated using SOLR cells.

text: Medicago truncatula

characteristic: organism

text: Virus-infected leaves

text: Virus-Infected Leaves

text: Plasmid

text: Lambda Zap


status: PUBLIC

characteristic: External Id

text: The Samuel Roberts Noble Foundation

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Medicago truncatula plants were infested with 300 spotted-aphids each. Tissue samples were collected by harvesting the entire shoots from 13 plants after 12, 24, 48, 72 and 96 hours of infestation. Total RNA was extracted from each sampling time and equal amounts of total RNA were pooled together for mRNA purification for cDNA library construction. The cDNA was directionally ligated into the Uni-Zap XR vector (Stratagene) and packaged using the Gigapack III Gold packaging extracts. Phagemids containing cDNA inserts were in vivo excised from the recombinant Uni-ZAP XR vector using ExAssist helper phage and the E. coli strain XL1-Blue MRF' (Stratagene). Excised plasmids were plated using SOLR cells.

characteristic: description

text: harvested after 12, 24, 48, 72 and 96 hours of infestation

tag: attribute

characteristic: dev-stage

text: Medicago truncatula

text: Aphid-infected shoots

characteristic: tissue-type

text: Aphid-Infected Shoots

characteristic: title

text: Plasmid

text: Lambda Zap

characteristic: vector


text: The Samuel Roberts Noble Foundation

text: live

text: Generic

text: Medicago truncatula root cell suspension culture elicited with 500 um methyl jasmonate. The cDNA was directionally ligated into the Uni-Zap XR vector (Stratagene) and packaged using the Gigapack III Gold packaging extracts. Phagemids containing cDNA inserts were in vivo excised from the recombinant Uni-ZAP XR vector using ExAssist helper phage and the E. coli strain XL1-Blue MRF' (Stratagene). Excised plasmids were plated using SOLR cells.

text: Seven-day old culture

text: Medicago truncatula

text: Root cell suspension culture

text: Methyl Jasmonate-Elicited Root Cell Suspension Culture

text: Plasmid

text: Lambda Zap


text: Shanghai Institute of Animal Parasitology, Chinese Academy of Agricultural Sciences

text: live

text: Generic

text: Egg subtracted cDNA library was constructed using cDNA from egg as tester and from adult worm as drivers. Subtractive PCRs were performed using the PCR-select cDNA subtractive kit according to the manufacture's protocol (clontech, palo Alto, CA) with the following modification. Xho I site and EcoR I site were respectively added to the 5' end of nested PCR primer 1 and nested PCR primer 2R. Sequence as follows: nested PCR primer 1 5' AGTTCTCGAGCGGCCGCCC GGGCAGGT 3', nested PCR primer 2R 5' TCGGGAATTCTGGTCGGTCGCGG CCGAGGT 3'. Thus PCR products can be directionally subcloned into the pCMV-SCRIPT ( XR ) vector ( stratagene ) and transformed into XL1-Blue electrophoration-competent cells ( stratagene ).

text: Egg

text: Oncomelania hupensis snails

characteristic: lab-host

text: Schistosoma japonicum

text: EcoRI

text: XhoI

text: Chinese mainland

characteristic: strain

text: Egg subtracted cDNA library

text: Plasmid

text: pCMV-SCRIPT


text: live

text: Generic

text: Male subtracted cDNA library using cDNA from male cDNA as testers and from egg as drivers. Subtractive PCRs were performed using the PCR-select cDNA subtractive kit according to the manufacture's protocol (clontech, palo Alto, CA) with the following modification. Xho I site and EcoR I site were respectively added to the 5' end of nested PCR primer 1 and nested PCR primer 2R. Sequence as follows: nested PCR primer 1 5' AGTTCTCGAGCGGCCGCCC GGGCAGGT 3', nested PCR primer 2R 5' TCGGGAATTCTGGTCGGTCGCGG CCGAGGT 3'. Thus PCR products can be directionally subcloned into the pCMV-SCRIPT ( XR ) vector ( stratagene ) and transformed into XL1-Blue electrophoration-competent cells ( stratagene ).

text: Adult worm

text: Rabbit

text: Schistosoma japonicum

text: EcoRI

text: XhoI

text: Male

characteristic: sex

text: Chinese mainland

text: Whole body

text: Male subtracted cDNA library

text: Plasmid

text: pCMV-SCRIPT


text: Department of Botany & Plant Sciences, University of California

text: live

text: Generic

text: Pomeroy OP

characteristic: cultivar

text: Plants were grown in the greenhouse at University of California, Riverside. The scion was an open-pollinated (very probably selfed) seedling of Poncirus trifoliata cv Pomeroy that was selected as homozygous for the Ctv resistance gene. The rootstock was sweet orange infected with citrus tristeza virus (CTV) isolate T514 over 1 year before sampling (CTV infects sweet orange, but not genotypes carrying the Ctv resistance gene. Shoots 10-30 cm long were harvested in October 2000, and the green phloem (bark) was removed and frozen quickly in dry ice. Total RNA was extracted using TriZol reagent (Gibco). Poly(A) RNA was purified, a cDNA library was made, and 0.5 million primary lambda cDNA clones were in vivo excised to give a population of pBluescript SK(-) phagemids. All steps to this point were performed in the ML Roose lab at the University of California, Riverside by X. Ye. Phagemids were plated, plasmid DNA purified, cDNA clones archived, and DNA sequences determined bi-directionally using an ABI3730 at the University of California Riverside Institute of Integrative Genome Biology Genomics Core Instrumentation Facility, (Choi, Kingan). Chromatogram files were downloaded by FTP by Close, then processed by Wanamaker (Close lab) using the HarvEST pipeline (http://harvest.ucr.edu) to remove vector and cloning oligo sequences and various contaminants, and to trim to a high quality region. Sequences that retained a phred 17 region of at least 100 bases were assembled, then chimeras were removed following manual inspection of assemblies (Close, Roose, Wanamaker). Sequences that survived all removal steps were submitted to GenBank.

text: 10 - 30 cm shoots

text: E. coli TJC121

text: Citrus trifoliata

text: EcoRI

text: XhoI

text: bark (with phloem)

text: Poncirus trifoliata CTV-challenged cDNA library - UCRPT01-UCR2

text: Phagemid pBluescript SK(-)

text: Lambda Uni-ZAP XR, excised phagemid


text: University of Missouri-Columbia

text: live

text: Generic

text: Funding: The production of EST's submitted in this project was funded by MSMC Grant 002245- 'Development and deployment of biotechnology tools for soybean improvement' to H.T. Nguyen as Principal Investigator. Sample collection and library construction: Dark grown soybean seedlings with primary roots were transplanted to high (-0.05MPa) or low water potential (-1.6MPa) vermiculite, and harvested at 5h and 48h after transplanting. Each root tip was divided into two segments (distances are from the junction of root apex and root cap): segment 1, 0-3mm; and segment 2, 3-11mm. Total RNA was extracted by the 'hot Phenol' method (Plant Molecular Biology manual. D5: 1-13, 2nd ed., 1997). This method worked in eliminating carbohydrate material present in the root tips. The integrity of the RNA was verified by denaturing agarose gels and spectrophotometry (ratio A260/280). Poly(A)+mRNA was isolated twice from total RNA using the Oligotex Direct mRNA kit (Qiagen). Poly(A)+ mRNA was converted to double-stranded cDNA and tagged by using modified Oligo(dT) primers. The double stranded cDNAs were size-selected (>500 bp). Size selected cDNAs were adaptored with EcoRI adaptors at both ends, and then digested with NotI. The cDNA was directionally cloned into EcoR1-NotI digested pBS II SK(+) phagemid vector (Stratagene) and electroporated into E.coli DH10B. The gmrtDrNS01 library was created by subtracting the well-watered soybean root library from the water-stressed (5h and 48h) soybean root libraries. The double stranded plasmid DNA from the primary library was converted to single-stranded circles and used as a template for PCR amplification. The purified PCR products, representing the entire cDNA population cloned were used as a driver for normalization. Hybridization between the single-stranded library and the PCR products was carried out for 44 hours at 30oC. Non-hybridized single-stranded DNA circles were separated from hybridized DNA rendered partially double-stranded and electroporated into DH10B E. coli. Clone requests: Requests for clones should be made to Dr. Henry Nguyen, National Center for Soybean Biotechnology, 1-87 Agriculture Bldg., University of Missouri-Columbia, MO, 65211. Email; nguyenhenry@missouri.edu

text: Droughted Roots

text: Glycine max

characteristic: organism

text: Cloning

text: pBS II SK(+)


characteristic: External Id

text: Boyce Thompson Institute for Plant Research

text: live

text: Generic

text: cDNA was prepared from polyA+ enriched RNA from roots harvested at 10, 17, 22, 31 and 38 days post-inoculation with Glomus versiforme. The cDNA was directionally ligated into the Unizap XR vector from Stratagene and packaged using Gigapack III Gold packaging extracts. Plasmids containing cDNA inserts were excised from the recombinant lambda-Zap phage using Ex-assist helper phage and propagated in XLOLR cells.

text: Roots harvested at 10, 17, 22, 31 and 38 days post-inoculation with Glomus versiforme

text: Medicago truncatula/Glomus versiforme mixed EST library

text: roots colonized with Glomus versiforme

text: Plasmid

text: Lambda Zap


text: live

text: Generic

text: The library was constructed according Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. Denatured RNA was size fractionated on a 1% agarose gel.First strand cDNA synthesis was primed with oligo-dT primer containing a Not I site .Double strand cDNA was size selected according to mRNA size fraction, ligated with EcoR I adaptor, digested with NotI and then cloned directionally into pYX-Asc vector. The library tag sequence located between the Not I site and the polyA tail is AATAATTACG. This library was created for the University Iowa Brain Anatomy Project (BMAP): 'Gene Discovery in the Developing Mouse Nervous System', supported by National Institute of Mental Health (NIMH).

text: newborn (1,5,15 days)

text: DH10B (T1 phage resistant)

text: Eye

characteristic: organ

text: Mus musculus

text: EcoR I

text: Not I

text: whole eye

text: Plasmid

text: pYX-Asc


text: Departement de Biochimie, Universite de Montreal

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: Tetrahymena thermophila

text: Amplicon Express - Conjugative Form


text: University of Kentucky

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: 1.5 year old

text: DH12S E. Coli cells (Invitrogen)

text: Equus caballus

text: male

text: Thoroughbred

text: articular cartilage

text: Equine Articular Cartilage cDNA Library

text: Plasmid

text: pGEM11Zf(+) (Promega)


text: Rothamsted Research

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Triticum aestivum

text: Triticum aestivum library (Howarth JR)


text: The University of Georgia, Department of Plant Biology

text: live

text: Generic

text: The library was prepared from polyA+ RNA isolated from seedlings of sorghum BTx623 grown in hydroponic culture. At 8 days of age, medium was purged with nitrogen gas for 3 hr prior to stopping aeration. Roots were harvested after 3, 27, and 72 hr. Material from all time points was combined prior to RNA isolation. Double-stranded cDNA was cloned unidirectionally into different DraIII sites of the pME18S-FL3 vector (5-prime DraIII site is CACTGTGTG, 3-prime DraIII site is CACCATGTG). XhoI excises the cDNA insert.

characteristic: description

text: DH10B-T1 phage-resistant E. coli

text: Root

text: Sorghum bicolor

text: XhoI

text: XhoI

text: Anaerobic roots

text: Plasmid


text: live

text: Generic

text: The library was prepared from polyA+ RNA from seedlings grown in hydroponic culture. At 8 days of age, medium was supplemented with gibberellic acid to a final concentration of 50 uM. In a parallel experiment, brassinolide was added to the medium at a final concentration of 500 nM. Shoots were harvested from gibberellic- and brassinolide-treated seedlings after 3, 27, and 72 hr and material from all time points and both treatments was combined prior to RNA isolation. Double-stranded cDNA was cloned unidirectionally into different DraIII sites of the pME18S-FL3 vector (5-prime DraIII site is CACTGTGTG, 3-prime DraIII site is CACCATGTG). XhoI excises the cDNA insert.

text: DH10B-T1 phage-resistant E. coli

text: Sorghum bicolor

text: XhoI

text: XhoI

text: GA- or brassinolide-treated seedlings

text: Plasmid


text: University of Minnesota Duluth

text: live

text: Generic

text: heart

text: Ictidomys tridecemlineatus

text: Spermophilus tridecemlineatus hibernating and active heart


text: Friedrich-Alexander University Erlangen-Nurnberg

text: live

text: Generic

text: Nickel and Carola

text: Kernels were germinated and subsequently grown in a mixture of SERAMIS and OIL DRI soil at 20 degr. C, 16h light and 50-60% rel. humidity, Roots were harvested after 7 days. PolyA-RNA was isolated from water treated roots and used to create a cDNA-library by means of the SMART cDNA library construction Kit (CLONTECH). cDNA Fragments were inserted into the vector Lambda TripleEx Sfi I. Subsequently, plasmids were obtained by in vivo excision according to manufacturers instruction. (GABI-Agrotech project)

text: E. coli BM25.8

text: Hordeum vulgare

text: Sfi IA

text: Sfi IB

text: roots

characteristic: tissue-type

text: GW


text: live

text: Generic

text: Nickel

text: roots, Seedlings were grown in a mixture of SERAMIS and OIL DRI soil at 20 degr. C, 16h light and 50-60% rel. humidity for 7 days, Roots were harvested 6, 24, 48, 72, and 96 hpi (hours post inoculation) with Fusarium culmorum KF 350. PolyA-RNA was isolated from water treated roots and used to create a cDNA-library by means of the SMART cDNA library construction Kit (CLONTECH). CDNA Fragments were inserted into the vector Lambda TripleEx Sfi I. Subsequently, plasmids were obtained by in vivo excision according to manufacturers instruction. (GABI-Agrotech Project)

text: E. coli BM25.8

text: Hordeum vulgare

text: Sfi IA

text: Sfi IB

text: roots

text: GNW

characteristic: title


text: live

text: Generic

text: Ingrid WT

text: seedlings were grown at 18 degr. C, 60 % rel. humidity, and a photoperiod of 16 h (100 microE * s-1 * m-2). A. 8 days, seedl. were treated with ASM (syn. BTH; 20 ppm soil drench). Leaf epid. was harv. 8, 24, 48, 72 hpt). PolyA-RNA was isolated from leaf epidermis and used to create a cDNA-library using the Time Saver cDNA-Synthesis Kit (Amersham). Normalisation was performed essentially as described by Ko (1990, Nucl. Acid Res. 16, 9877) with some modification of Kohchi et al. (1995, Plant J. 8, 771-776). Normalised cDNAs were digested with NotI and cloned into NotI digested pCRblunt vector. (GABI-Agrotech Project)

text: Hordeum vulgare

text: NotI

text: NotI

text: leaf epidermis

text: GAN

text: pCR blunt (Invitrogen)

characteristic: vector


text: live

text: Generic

text: backcross: Ingrid mlo5 and Ingrid MLG

text: seedlings were grown at 18 degr. C, 60 % rel. humidity, and a photoperiod of 16 h (100 microE * s-1 * m-2). A. 8 days, seedl. were treated with cuture filtrate of Bipolaris sorokiniana. Leaf epid. was harv. 6, 12, 24, 48 hpt,=. PolyA-RNA was isolated from leaf epidermis and used to create a cDNA-library using the Time Saver cDNA-Synthesis Kit (Amersham). Normalisation was performed essentially as described by Ko (1990, Nucl. Acid Res. 16, 9877) with some modification of Kohchi et al. (1995, Plant J. 8, 771-776). Normalised cDNAs were digested with NotI and cloned into NotI digested pCRblunt vector. (GABI-Agrotech Project)

characteristic: lab-host

text: Hordeum vulgare

text: NotI

text: NotI

text: leaf epidermis

text: GBN

text: pCR blunt (Invitrogen)


text: live

text: Generic

text: Carola

text: Seedlings were grown in a mixture of SERAMIS and OIL DRI soil at 20 degree C, 16h light and 50-60% rel. humidity for 7 days, Roots were harvested 6, 24, 48, 72, and 96 hpi (hours post inoculation) with Fusarium culmorum KF 350. PolyA-RNA was isolated from water treated roots and used to create a cDNA-library by means of the SMART cDNA library construction Kit (CLONTECH). CDNA Fragments were inserted into the vector Lambda TripleEx Sfi I. Subsequently, plasmids were obtained by in vivo excision according to manufacturers instruction. (GABI-Agrotech Project)

text: E. coli BM25.8

text: Hordeum vulgare

text: Sfi IA

text: Sfi IB

text: roots

text: GCW


text: Massachusetts General Hospital

text: live

text: Generic

text: RT-PCR was performed on human fetal brain first-strand cDNA, using specific, overlapping primer sets designed across the entire coding sequence of Pam, to detect alternative splicing in transcripts of this gene. Total RNA from human fetal brain was isolated by the lithium chloride/urea method. First-strand cDNA synthesis from 20 ug of total RNA was performed using the SuperScript First-Strand Synthesis System for RT-PCR kit (Invitrogen). To identify the cDNA sequence of the multiple RT-PCR species, amplification products were T-cloned into the pCR2.1 vector (Invitrogen), and individual clones were isolated and sequenced in both directions.

text: Fetal

characteristic: dev-stage

text: Brain

text: Homo sapiens

text: Human fetal brain first-strand cDNA


text: University of California

text: live

text: Generic

text: Pomeroy

text: Plant materials were prepared by Federici (Roose lab) with advice from Parker. Seedlings 138 days after sowing were about 20-23 cm in height, bearing 8-12 leaves. On May 26, 2004, plants were washed free of soil with a stream of water, then placed upright with the roots submerged in two tanks of nutrient solution. The solution was sufficient in all major and minor nutrients and buffered with MES at about pH 6.95. The plants were maintained in this until June 14, 2004, when the solution was changed to one with only 20 micromolar iron, chelated with EDTA to induce iron deficiency. The pH was maintained at 6.99 by sodium carbonate/CO2 buffering. This solution was replaced on July 6, 2004. Roots from three plants were sampled on June 16, June 21, July 1 and July 28, 2004. Roots were collected by removing the plant from the nutrient solution, blotting off excess moisture with a paper towel, then cutting off the top of the plants. Three plants were pooled in one aluminum foil packet, and frozen between two sheets of dry ice. The time between removal from solution and freezing on dry ice did not exceed one minute. The frozen tissue was stored in these foil packs at -80oC. This sampling strategy did not correspond to initiation of stress in the plant, but only to when the plants entered the low iron solution. The actual stress was not initiated until the internal iron reserve was depleted. By the July 1 sampling date, slight iron deficiency symptoms were apparent. By the final sampling date, clear iron deficiency symptoms were present. By the July 1 sampling date, the roots showed growth of Thielaviopsis brevicola, a fungus. Other than what came away when the roots were blotted with paper towels, no effort was made to remove the fungus because it is not just a surface contaminant, but grows within the roots as well. Mandal and Fenton (Close lab) purified RNA using TRIzol, poly(A) mRNA using an Oligotex mRNA Kit (Qiagen), produced a primary cDNA library using a lambda ZAP XR cDNA Synthesis Kit (Stratagene), then mass-excised 0.5 million pfu from the primary library to produce a phagemid population. The library was made from equal portions of RNA from each of the four collection dates. Phagemids were plated, plasmid DNA purified, cDNA clones archived, and DNA sequences determined bi-directionally using an ABI3730 at DNA Landmarks (Landry, Hubert, Laforest, Landry, Ligonde). Chromatogram files were downloaded by FTP by Close, then processed by Wanamaker (Close lab) using the HarvEST pipeline (http://harvest.ucr.edu) to remove vector and cloning oligo sequences and various contaminants, and to trim to a high quality region. Sequences that retained a phred 17 region of at least 100 bases were assembled, then chimeras were removed following manual inspection of assemblies (Close, Roose, Wanamaker). Sequences that survived all removal steps were submitted to GenBank.

text: seedling

text: E. coli TJC121

text: Citrus trifoliata

text: EcoRI

text: XhoI

text: root

text: Poncirus trifoliata Roots with Iron Deficiency - UCRPT02-UCR1

text: Phagemid pBluescript SK(-)

text: Lambda Uni-ZAP XR, excised phagemid


text: live

text: Generic

text: The library was prepared from polyA+ RNA from the roots of 1-year-old loblolly pine (Pinus taeda) cuttings that were rooted and then planted in washed sand. The rooted cuttings were maintained for 50 days (May 2003 harvest) under ambient conditions in a local greenhouse. They were kept on a weekly regimen of 0.5x nutrient-complete Hoagland's solution and supplemented with additional water sufficient to maintain a 15% soil moisture content. Twenty-four hours (24h) prior to harvesting roots for mRNA preparation, the potted trees were placed at 4 C in a growth chamber under a diurnal light cycle. Double-stranded cDNA was cloned unidirectionally into pSL1180. Inserts can be excised with EcoRI (5' end) and XhoI (3' end).

text: DH10B-T1 phage-resistant E. coli

text: Root

text: Pinus taeda

text: EcoRI

text: XhoI

text: 3 CCLONES

characteristic: strain

text: Root cold


text: Institut Universitaire Europeen de la Mer, Universite de Bretagne Occidentale

text: live

text: Generic

text: sea bass challenged in seawater and freshwater

text: juvenile

text: Dicentrarchus labrax

text: gills and intestine

text: subtracted Libraries from sea bass challenge in seawater and freshwater


text: University of California

text: live

text: Generic

text: Swingle

characteristic: cultivar

text: Twelve seedlings were transplanted from cones to 4 inch pots of sand mix, then grown in greenhouses (Darsow, Becker lab). Citrus nematodes (Tylenchulus semipenitrans) were extracted from contaminated soil from the UC Riverside Citrus Research Center then suspended in water and added to pots (Darsow). Growing roots were collected by clipping them away from the old root ball 24 hr and 4 days after inoculation (Federici). Total RNA was extracted using Concert Plant RNA Reagent (Invitrogen)followed by RNeasy (Qiagen), an equal amount of RNA was pooled from each of the two collection times, poly(A) RNA was purified using Oligotex (Qiagen), a cDNA library was made using a cDNA synthesis kit (Stratagene), then 0.45 million primary lambda cDNA clones were in vivo excised to give a population of pBluescript SK(-) phagemids (Fenton, Close lab). Phagemids were plated, plasmid DNA purified, cDNA clones archived, and DNA sequences determined bi-directionally using an ABI3730 at the University of California Riverside Institute of Integrative Genome Biology Genomics Core Instrumentation Facility, (Choi, Kingan). Chromatogram files were downloaded by FTP by Close, then processed by Wanamaker (Close lab) using the HarvEST pipeline (http://harvest.ucr.edu) to remove vector and cloning oligo sequences and various contaminants, and to trim to a high quality region. Sequences that retained a phred 17 region of at least 100 bases were assembled, then chimeras were removed following manual inspection of assemblies (Close, Roose, Wanamaker). Sequences that survived all removal steps were submitted to GenBank.

text: seedling

text: E. coli TJC121

text: Citrus x paradisi x Citrus trifoliata

text: EcoRI

text: XhoI

text: root

text: Swingle citrumelo nematode-challenged root cDNA library - UCRCP01

text: Phagemid pBluescript SK(-)

text: Lambda Uni-ZAP XR, excised phagemid


text: University of California

text: live

text: Generic

text: Parent Washington Navel

text: Parent Washington navel orange embryogenic callus was established from undeveloped ovules of ca. 10 mm diameter young fruits under open pollination on Murashige-Skoog medium at 25oC with 16 h light in a tissue culture room. Embryogenic callus, globular and heart stage embryoids were pooled in approximately equal portions in RNAlater (Ambion), then RNA was extracted using TRIZOL Reagent (Invitrogen). Poly(A) RNA was purified from 500 microgram of total RNA using Qiagen Oligotex. A primary cDNA library was produced using a lambda ZAP XR cDNA Synthesis Kit (Stratagene). These steps were performed by Xinrong Ye (Roose lab, UC Riverside). One million pfu from the primary library were mass excised to produce a phagemid population by Raymond Fenton (Close lab, UC Riverside). Phagemids were plated, plasmid DNA purified, cDNA clones archived, and DNA sequences determined bi-directionally using an ABI3730 at DNA Landmarks (Landry, Hubert, Laforest, Landry, Ligonde). Chromatogram files were downloaded by FTP to UC Riverside (by Close), then processed at UC Riverside (by Wanamaker, Close lab) using the HarvEST pipeline (http://harvest.ucr.edu) to remove vector and cloning oligo sequences and various contaminants, and to trim to a high quality region. Sequences that retained a phred 17 region of at least 100 bases were assembled, then chimeras were removed following manual inspection of assemblies (Close, Roose, Wanamaker). Sequences that survived all removal steps were submitted to GenBank.

text: Embryogenic and embryoid

text: E. coli TJC121

text: Citrus sinensis

characteristic: organism

text: EcoRI

text: XhoI

text: Callus

text: Parent Washington Navel Orange Callus cDNA Library UCRCS08-3

text: Phagemid pBluescript SK(-)

text: Lambda Uni-ZAP XR, excised phagemid


characteristic: External Id

text: University of California

text: live

text: Generic

text: Madame Vinous

text: Samples included bark, leaf and petiole from infected trees of Madame Vinous sweet orange. These trees were growing in pots in greenhouses at the USDA Citrus Repository (Nielsen), UC Riverside. The trees were infected with various pathogens. A total of 14 trees were sampled and pooled. Two trees each were infected with a different strain of Citrus psorosis virus, Citrus viroid IIb, Spiroplasma citri, or 'Citrus Dweet mottle virus', two trees were infected with a seedling yellows strain of Citrus tristeza virus, one tree was co-infected with 'Citrus concave gum virus' plus Citrus exocortis viroid, and two trees were co-infected with Citrus tatter leaf virus and 'Citrus vein enation virus'. Federici (Roose lab) collected the samples, after consulting with Lee, Krueger and Roose. Samples were collected from three or four young branches per tree. The bark had hardened but was still green and the diameter of the twig was less than approximately 5 mm. The tissue was not washed. The two trees infected with Vein enation/Tatterleaf had some mites on the leaves; the infestation was very light. These were wiped off before freezing the tissue, but mite material may have been included in the sample. Twenty young fully expanded leaf blades with the petioles removed were collected from each tree and placed together in one foil packet submerged in liquid nitrogen. The petioles from these leaves and another twenty from the same plant were frozen in another foil pack. Then the bark was pulled off the branches and chopped into pieces no longer than 3 cm and frozen together in a third packet. The bark from each plant was not weighed to equalize it, but the amounts appeared roughly equal. Mandal and Fenton(Close lab) purified RNA by a TRIzol method, pooled an equal quantity of RNA from each of the three samples, purified poly(A) mRNA using a PolyATtrack mRNA Isolation System IV (Promega), produced a primary cDNA library using a lambda ZAP XR cDNA Synthesis Kit (Stratagene), then mass-excised one million pfu from the primary library to produce a phagemid population. Phagemids were plated, plasmid DNA purified, cDNA clones archived, and DNA sequences determined bi-directionally using an ABI3730 at DNA Landmarks (Landry, Hubert, Laforest, Landry, Ligonde). Chromatogram files were downloaded by FTP to UC Riverside (by Close), then processed at UC Riverside (by Wanamaker, Close lab) using the HarvEST pipeline (http://harvest.ucr.edu) to remove vector and cloning oligo sequences and various contaminants, and to trim to a high quality region. Sequences that retained a phred 17 region of at least 100 bases were assembled, then chimeras were removed following manual inspection of assemblies (Close, Roose, Wanamaker). Sequences that survived all removal steps were submitted to GenBank.

text: Trees in pots

text: E. coli TJC121

text: Citrus sinensis

text: EcoRI

text: XhoI

text: Leaf, petiole, bark

text: Madame Vinous Sweet Orange Multiple Pathogen-Infected cDNA Library UCRCS10

text: Lambda Uni-ZAP XR, excised phagemidV_TYPE: Phagemid pBluescript SK(-)


text: Indian Institute of Horticultural Research

text: live

text: Generic

text: Alphonso

text: Used PCR based subtractive hybridization to isolate fruit tissue specific mRNA in ripening mango fruits cv. Alphonso

text: Ripened fruits

text: Mangifera indica

text: Fruit pulp

text: Mango fruit tissue EST library

text: plasmid


text: University of Puerto Rico

text: live

text: Generic

text: Larvae from Paul Brakefield's stock in Leiden (The Netherlands), wing discs preserved in RNAlater at -80C, RNA extracted from the wingdiscs of 5 crawlers (179 ug total RNA) and 5 prepupae (152 ug total RNA) using Trizol(R) protocol and percussion. mRNA selection using MicroPolyAPurist and cDNA synthesis and ligation using SuperScript Plasmid & Gateway technology. Sequencing at Institute of Evolutionary Biology, The University of Edinburgh. Trace and submission files created using trace2dbEst.

text: late 5th instar, prepupae

text: Heliconius melpomene

text: NotI

text: SalI

text: Leiden Stock

text: wing disc

text: Heliconius melpomene late 5th instar wing disc

text: plasmid


text: The University of Georgia, Department of Plant Biology

text: live

text: Generic

text: The library was prepared from polyA+ RNA from the roots of 1-year-old loblolly pine (Pinus taeda) cuttings that were rooted and then planted in washed sand. The rooted cuttings were maintained for 220 days (October 2003 harvest) under ambient conditions in a local greenhouse. They were kept on a weekly regimen of 0.5x nutrient-complete Hoagland's solution and supplemented with additional water sufficient to maintain a 15% soil moisture content. Thirty days (30d) prior to harvesting roots for mRNA preparation, the potted tree stems were punctured at the root:shoot junction with an 18 gauge syringe needle to permit attachment of a 200 ul plastic pipette tip. The open-ended pipette tip was kept filled with a 0.1% aq. solution of paraquat dichloride until the trees were harvested. Double-stranded cDNA was cloned unidirectionally into pSL1180. Inserts can be excised with EcoRI (5' end) and XhoI (3' end).

text: DH10B-T1 phage-resistant E. coli

text: Root

characteristic: organ

text: Pinus taeda

text: EcoRI

text: XhoI

text: 2 CCLONES

text: Roots treated with paraquat


text: USDA, ARS, National Center for Cool and Cold Water Aquaculture

characteristic: INSDC center name

text: live

text: Generic

text: This is a normalized pituitary library created by Mathew L. Rise.

text: Oncorhynchus mykiss

text: NCCCWA 07RT

text: Plasmid

text: pBluescript SK+


characteristic: INSDC first public

text: live

text: Generic

text: INRA testis library made by M. Govoroun, F. Le Gac, and Y. Guiguen.

text: Oncorhynchus mykiss

text: NCCCWA 09RT

text: Plasmid


text: Tokyo University of Marine Science and Technology

characteristic: INSDC last update

text: live

text: Generic

text: adult

text: Paralichthys olivaceus

text: spleen

text: Japanese flounder spleen cDNA library

text: pSPORT 1


text: National Fisheries Research & Development Institute

text: live

characteristic: INSDC status

text: Generic

text: Adult

text: E.coli SOLR

text: Skin

text: Haliotis discus

text: EcoR I

text: Xho I

text: Haliotis discus cDNA library (SKN)

text: Phagemid

text: pBluescript SK(-)


text: live

text: Generic

characteristic: NCBI submission model

text: Adult

text: E.coli SOLR

text: Gill

text: Haliotis discus

text: EcoR I

text: Xho I

text: Haliotis discus cDNA library (GIL)

text: Phagemid

text: pBluescript SK(-)


text: University Clinic, Heidelberg

text: live

text: Generic

characteristic: NCBI submission package

text: Homo sapiens

text: male

characteristic: sex

text: kidney

text: Cloned PCR products library human kidney


text: National Veterinary Institute

text: live

text: Generic

text: Subtractive library from vaccinated fish, tester; day 2 post vaccination, driver: unvaccinated fish. CLONTECH PCR-Select cDNA Subtraction Kit

characteristic: description

text: Parr

text: Salmo salar

text: Head kidney

characteristic: tissue-type

text: Plasmid

text: pDrive (Qiagen)


text: National Veterinary Institute

text: live

text: Generic

text: Subtractive library from vaccinated fish, tester; day 8 post vaccination, driver: unvaccinated fish. CLONTECH PCR-Select cDNA Subtraction Kit

text: Parr

text: Salmo salar

text: Blood

characteristic: title

text: Plasmid

text: pDrive (Qiagen)

characteristic: vector


text: National Veterinary Institute

text: live

text: Generic

text: Subtractive library from vaccinated fish, tester; day 19 post vaccination, driver: day 2 post vaccination. CLONTECH PCR-Select cDNA Subtraction Kit

text: Parr

characteristic: dev-stage

text: Salmo salar

text: Head kidney

text: Plasmid

text: pDrive Qiagen


text: National Eye Institute

text: live

text: Generic

text: Approximately 1mg total RNA was extracted from 100 adult mouse whole eyes. A directionally cloned cDNA library in the pCMVSPORT6 vector (Invitrogen) was constructed at Bioserve Biotechnology (Laurel MD) essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. cDNA was cloned in Not I/Sal I sites. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC).

text: Adult

characteristic: lab-host

text: Eye

text: Mus musculus

text: No3 (C3H background)

characteristic: strain

text: Whole eye (retinal degeneration)

text: No3 mouse (cataract/retinal degeneration) whole eye, unamplified: ob/oc

text: Plasmid


text: National Eye Institute

text: live

text: Generic

text: Approximately 1mg total RNA was extracted from 100 adult mouse whole eyes. A directionally cloned cDNA library in the pSPORT1 vector (Invitrogen) was constructed at Bioserve Biotechnology (Laurel MD) essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. cDNA was cloned in Not I/Sal I sites. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC).

text: Adult

text: Eye

text: Mus musculus

characteristic: organism

text: Whole eye

text: Mouse Whole eye, equalized: ja/jb/jc

text: Plasmid


characteristic: External Id

text: Medical University of South Carolina

text: live

text: Generic

text: gills

characteristic: cell type

text: Penaeus vannamei

text: EcoRI

text: EcoRI

text: Plasmid


text: Children's Memorial Research Center

characteristic: INSDC center name

text: live

text: Generic

text: The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is GAAGGCTAGT. Tissue was obtained from the Provasoli-Guillard National Center for Culture of Marine Phytoplankton (CCMP).

text: DH10B (Life Technologies) (T1 phage resistant)

text: Emiliania huxleyi

text: EcoR I

text: Not I

text: CCMP 371

text: Plasmid

text: pT7T3-Pac (Pharmacia) with a modified polylinker


text: Children's Memorial Research Center

characteristic: INSDC first public

text: live

text: Generic

text: UI-EH-HG1 is a normalized library derived from UI-EH-HG0. The library was constructed and normalized according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is GAAGGCTAGT. Tissue was obtained from the Provasoli-Guillard National Center for Culture of Marine Phytoplankton (CCMP).

text: Emiliania huxleyi

text: EcoR I

text: Not I

text: CCMP 371

text: Plasmid


text: Children's Memorial Research Center

characteristic: INSDC last update

text: live

text: Generic

text: UI-EH-HG2 is a normalized library derived from UI-EH-HG1. The library was constructed and normalized according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is GAAGGCTAGT. Tissue was obtained from the Provasoli-Guillard National Center for Culture of Marine Phytoplankton (CCMP).

text: Emiliania huxleyi

text: EcoR I

text: Not I

text: CCMP 371

text: Plasmid


text: Faculty of Veterinary medicine, Universite de Montreal

text: live

characteristic: INSDC status

text: Generic

text: granulosa and oocyte

text: PCR fragment; cDNA library made from granulosa cells and oocytes of dominant follicles (DF) collected at day 5 of the estrous cycle that was subtracted from granulosa cells and oocytes of ovulatory follicles obtained 23 hours (OF) after human chorionic gonadotropin injection.

text: dominant follicle

text: ovary

characteristic: organ

text: Bos taurus

text: female

text: Hosltein

text: Bos taurus subtracted cDNA of granulosa cells and oocytes from dominant follicles (DF-OF)


text: Beijing Hospital

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: identified by differential display between normal thyroid tissue and thyroid tissue of hyperthyroidism patients

text: Homo sapiens

text: thyroid

text: Homo sapiens thyroid differential display


text: Research Institute for Microbial Diseases, Osaka University

text: live

text: Generic

text: Mus musculus

text: male

characteristic: sex

text: ICR

text: testis

text: lambda gt11 mouse testis cDNA


text: Kyoto University

text: live

text: Generic

text: Euphorbia tirucalli

text: callus induced from stem

characteristic: tissue-type

text: Euphorbia tirucalli callus induced from stem


text: School of Medicine, USP

text: live

text: Generic

text: total leukocytes

text: Peripheral blood leukocytes were pooled from SLE patients and used for total RNA extraction which served to synthesize cDNA by reverse transcription using random primers (random hexameric primers - Promega). A cDNA library was prepared using the SuperScriptTM Plasmid System with GatewayTM Technology for cDNA Synthesis and Cloning (GIBCO) with modifications. The cDNA fragments obtained were cloned in pUC18 vector using the SureClone Kit (Amersham- Pharmacia Biotech).

characteristic: description

text: peripheral blood leukocytes

characteristic: isolate

text: Homo sapiens

text: EcoRI

text: Hind-III

text: Random primed cDNA library from Homo sapiens peripheral blood leukocytes mRNA

characteristic: title

text: plasmid


text: Centre de Recherche du Centre hospitalier de l'Universite Laval (CHUL)

text: live

text: Generic

text: TP31 cell line

characteristic: cell-line

text: mRNAs derived from the TP31 cell line and from uveal melanocytes were isolated with the Oligotex mRNA kit (Qiagen). cDNA was synthesized using the SMART PCR cDNA Synthesis kit (BD Biosciences Clontech). Suppressive subtractive hybridization (SSH) was then performed between TP31 cell line and human uveal melanocytes using the PCR-Select cDNA Subtraction kit (BD Biosciences Clontech). In the forward subtraction, the TP31 cell line was used as tester and the uveal melanocytes as driver (TP31-uveal melanocytes), and conversely, in the reverse subtraction. Two hybridization steps were performed. These cDNAs were then amplified by two suppressive PCR amplifications with the Advantage cDNA PCR kit (BD Biosciences Clontech). The forward subtracted fragments were then inserted into the T/A cloning vector pGEM-T Easy (Promega).

text: DH5 alpha

characteristic: lab-host

text: eye

text: Homo sapiens

text: male

text: primary uveal melanoma tumor

text: Human TP31 Cell Line Library

text: plasmid

text: pGEM-T Easy (Promega)

characteristic: vector


characteristic: External Id

text: National Cancer Institute / NIH

text: live

text: Generic

text: human embryonic stem cells

text: RNA obtained from human embryonic stem cells isolated from the inner cell mass of blastocyst stage embryos. Cell line id and NIH Registry designation is BGO1. Positive for SSEA3, SSEA4, Tra 1-60, Tra 1-81, CD9, Alk Phos, Oct4 and Nanog expression; negative for SSEA1 expression. Passage number 21. cDNA primed using oligo-dT primer: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3' and cloned into the EcoRV/NotI sites of pExpress-1. This primary library is non-normalized (normalized primary library is NIH_MGC_261). It was constructed by Express Genomics (Frederick, MD). Sequence ends have been trimmed to exclude vector and regions below Phred quality 16. Note: this is a Mammalian Gene Collection library.

text: DH10B-T1 phage-resistant E. coli

text: Homo sapiens

characteristic: organism

text: NotI

text: EcoRV

text: male

text: human embryonic stem cells

text: Plasmid


text: Institute for Molecular and Cellular Regulation, Gunma University

characteristic: INSDC center name

text: live

text: Generic

text: Homo sapiens

text: pancreatic islet

text: Homo sapiens pancreatic islet


text: National Agricultural Research Center for Hokkaido Region

characteristic: INSDC first public

text: live

text: Generic

text: Beta vulgaris

text: hypocotyl, root

text: Beta vulgaris hypocotyl, root


text: National Institute for Genetic Engineering and Biotechnology

characteristic: INSDC last update

text: live

text: Generic

text: Twenty one-day old seedlings was cultured in 1/2 strength MS medium without or with salt (600 mM NaCl) for 10 days. Twelve differentially expressed cDNA bands were isolated by using differential display amplified fragment length polymorphism (DD-AFLP) method.

text: 31 day old seedlings at 5 leaf stage

characteristic: dev-stage

text: Shoot

text: Aeluropus lagopoides

text: leaf

text: Differential display amplified fragment length polymorphism (DD-AFLP) method


text: Albert-Ludwigs-Universitaet Freiburg

text: live

characteristic: INSDC status

text: Generic

text: cDNA synthesis was performed using SuperScript system (Invitrogen) and directionally-cloned (Sal I at the 5'-end and Not I at the 3'-end) into pSPORT1 (U12390.1) country: Portugal:Braga

text: floral spathe whitening and regreening

text: Escherichia coli strain TOP10

text: Zantedeschia aethiopica

text: floral spathe

text: ZAAE floral spathe MPIZ-ADIS-062 cDNA


text: Universiti Kebangsaan Malaysia

text: live

text: Generic

characteristic: NCBI submission model

text: Juvenile

text: Spleen

characteristic: organ

text: Lates calcarifer

text: Male (hemaphrodite)

text: LcSpE

text: Phagemid

text: Uni-Zap XR Vector


text: Instituto Butantan

text: live

text: Generic

characteristic: NCBI submission package

text: 4ug of mRNA from Lonomia obliqua bristles were primed with oligo- (dT) and reverse transcribed to cDNA using Superscript Plasmid System for cDNA Synthesis and Cloning (Invitrogen). The cDNAs were selected by size (400-800 pb and up 800 pb) in agarose gel electrophoresis, linked to Eco RI adapters and directionally cloned in pGEM11Zf+ vector (Promega). ESTs were generated from random clones and grouped in unique sequences. The putative identification of each EST or cluster was obtained through Blast searches (e-value < e-05).

text: bristles

text: Lonomia obliqua

text: Eco RI

text: Not I

text: bristles

text: Caterpillar Lonomia obliqua library LOA

text: plasmid


text: Instituto Butantan

text: live

text: Generic

text: bristles

text: Lonomia obliqua

text: Eco RI

text: Not I

text: bristles

characteristic: tissue-type

text: Caterpillar Lonomia obliqua library LOB

text: plasmid


text: Biosystems Department, Riso National Laboratory

text: live

text: Generic

characteristic: cultivar

text: Lolium perenne

text: aerial part of vegetative grown plants

text: Lolium cDNA UNI-ZAP XR library

text: Phage

text: UNI-ZAP XR


text: National Agricultural Research Organization

text: live

text: Generic

text: Abyara

text: Three leaf stage

text: Glycine max

text: Flooded roots

text: Glycine max Abyara mRNA differential display

text: Plasmid

text: pCR II TOPO vector


text: Stanford University School of Medicine

text: live

text: Generic

text: adult

text: Homo sapiens

text: female

characteristic: sex

text: cervix

text: Homo sapiens cervix adult


text: Soochow University

text: live

text: Generic

text: Homo sapiens

text: T lymphocyte

text: Homo sapiens T lymphocyte

characteristic: title


text: National Cancer Institute / NIH

text: live

text: Generic

text: human embryonic stem cells

characteristic: cell type

characteristic: cell-line

text: RNA obtained from human embryonic stem cells isolated from the inner cell mass of blastocyst stage embryos and differentiated to an early neural progenitor cell type. Cell line id and NIH Registry designation is BGO1. Positive for Nestin and Musashi expression. Passage number 18. cDNA primed using oligo-dT primer: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3' and cloned into the EcoRV/NotI sites of pExpress-1. This primary library is non-normalized (normalized primary library is NIH_MGC_259). It was constructed by Express Genomics (Frederick, MD). Sequence ends have been trimmed to exclude vector and regions below Phred quality 16. Note: this is a Mammalian Gene Collection library.

characteristic: description

text: DH10B-T1 phage-resistant E. coli

text: Homo sapiens

text: NotI

text: EcoRV

text: male

text: embryonic stem

text: Plasmid


characteristic: External Id

text: Chinese National Human Genome Center at Shanghai

text: live

text: Generic

text: Schistosoma japonicum

text: SJA


text: University of Xinjiang Shehezi

text: live

text: Generic

text: adult

text: Ovis aries

characteristic: organism

text: skin

text: adult sheep skin library


text: Xinjiang Academy of Agricultural Reclamation

characteristic: INSDC center name

text: live

text: Generic

text: Ovis aries

text: ovis aries wool growth cDNA


text: Centro de Biotecnologia

characteristic: INSDC first public

text: live

text: Generic

text: cDNA synthesis products from larval (tetrathyridia) and segmented forms of Mesocestoides corti were generated using SMART technology. PCR-amplified cDNAs were digested with Sau3AI and a cDNA RDA methodology was carried out using cDNA from segmented worms as tester for this library. After two rounds of subtraction using a driver/tester ratio of 100/1 and 800/1 for the first and second round, respectively, cDNA sequences were cloned into pBluescript II SK(+) digested with EcoRV.

text: segmented worms

text: Mesocestoides corti

text: EcoRV

text: dp2forward library

text: Plasmid

text: pBluescript II SK(+) (Stratagene) (gi:58063)

characteristic: vector


text: Centro de Biotecnologia

characteristic: INSDC last update

text: live

text: Generic

text: cDNA synthesis products from larval (tetrathyridia) and segmented forms of Mesocestoides corti were generated using SMART technology. PCR-amplified cDNAs were digested with Sau3AI and a cDNA RDA methodology was carried out using cDNA from tetrathyridia as tester for this library. After two rounds of subtraction using a driver/tester ratio of 100/1 and 800/1 for the first and second round, respectively, cDNA sequences were cloned into pBluescript II SK(+) digested with EcoRV.

text: larval (tetrathyridia)

characteristic: dev-stage

text: Mesocestoides corti

text: EcoRV

text: dp2reverse library

text: Plasmid


text: Nanjing Agricultural University

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: mycelium in V8 juice were as driver and those in the surface of its host were as tester. We constructed two libraries name as forward and reverse library. the sequences with the name labeled with 'f' was originated from forward library and others from reverse library.

text: mycelium in V8 juice and host plant soybean

text: soybean cultivar Hefeng35

characteristic: lab-host

text: Phytophthora sojae

characteristic: strain

text: subtracted suppression library of Phytophthora sojae during compatible interaction with soybean

text: pMD 18 T-vector


text: College of Marine Life Sciences, Ocean University of China

text: live

text: Generic

characteristic: NCBI submission package

text: Neopyropia yezoensis

text: PY Sporophyte cDNA Library


text: University of Queensland

text: live

text: Generic

text: eggs, embryos, larvae, postlarvae

text: XL1 Blue

text: Haliotis asinina

text: Haliotis asinina developmental cDNA library

text: Lambda TripleX


text: Genoplante

text: live

text: Generic

text: XRQ

characteristic: cultivar

text: Helianthus annuus

text: cotyledons

characteristic: tissue-type

text: HaDisCtXRQvsPh


text: Genoplante

text: live

text: Generic

text: xrq

text: Helianthus annuus

characteristic: title


text: Genoplante

text: live

text: Generic

text: XRQ

text: Helianthus annuus

text: cotyledons

text: HaDisPhvsCtXRQ


text: Genoplante

text: live

text: Generic

text: Helianthus annuus

text: cotyledons


text: Genoplante

text: live

text: Generic

text: Helianthus annuus


text: Genoplante

text: live

text: Generic

text: Helianthus annuus

text: 7 days after self-pollination embryo


text: Genoplante

text: live

text: Generic

text: Helianthus annuus

text: 28 days after self-pollination embryo


text: Genoplante

text: live

text: Generic

text: Helianthus annuus

text: 4 days after self-pollination embryo


text: Medical University of South Carolina

text: live

text: Generic

text: Spleen

characteristic: cell type

text: Anas platyrhynchos

text: Anas platyrhynchos EST Library (L99-05) at marinegenomics.org

text: Plasmid

text: LambdaZap


text: University of Victoria

text: live

text: Generic

text: Library Creator: Susan Douglas Tissues from liver, kidney and spleen

characteristic: description

text: Salmo salar


characteristic: External Id

text: live

text: Generic

text: Total RNA samples were prepared individually from each tissue, checked for quality, then pooled for construction and normalization of the cDNA library by Invitrogen. Plasmids were isolated and inserts end-sequenced by the Marine DNA Sequencing and Analysis Facility at the Center for Marine Functional Genomic Studies, Mount Desert Island Biological Laboratory (J.N. Forrest, Jr., Director). Traces were processed for submission to dbEST by trace2dbest software (Parkinson, Anthony and Blaxter, unpublished software). The project was supported by NIH Grant Number P20-RR-16463 from the INBRE Program of the National Center for Research Resources (P.H. Hand, PI).

text: Adult

text: Carcinus maenas

text: Gill, hypodermis, heart, hepatopancreas, antennal gland, brain, testis, muscle

text: Green Shore Crab Multiple Tissue, Normalized

text: pCMV Sport 6.1


text: University of Victoria

text: live

text: Generic

text: Library Creator: Susan Douglas

text: Salmo salar

characteristic: organism

text: pBlueScriptIISK+


text: Institute of Zoology, Zoological Society of London

characteristic: INSDC center name

text: live

text: Generic

text: Suppression Subtractive Hybridization was performed to create a subtracted cDNA library to screen by Differential Screening for sequences associated with queen and worker development in early and late larval instars.

text: larva

text: E.coli TOP10F'

text: Bombus terrestris

text: EcoRI

text: EcoRI

text: female

characteristic: sex

text: dalmatinus

text: whole body

text: Bombus terrestris larval caste mRNA


text: UC Davis, Department of Pomology

characteristic: INSDC first public

text: live

text: Generic

text: Albedo

text: Washington Navel Orange

text: Developing citrus fruits were harvested from trees growing in the 'Citrus variety collection' in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected on July 29, 2003, between 8 to 9 am and stored at 4C. The albedo tissue was dissected out of developing fruit (80 DAFB) and used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The primary library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes.

text: Developing fruit albedo-collected July 29, 2003

text: Fruit

characteristic: organ

text: Citrus sinensis

text: SfiIA

text: SfiIB

text: Developing fruit albedo at 80 DAFB in Vulnus5 vector

text: Plasmid

characteristic: vector


text: UC Davis, Department of Pomology

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Washington navel orange

text: Mature citrus fruits were harvested from trees growing in the 'Citrus variety collection' in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected in January 2002, between 1 and 3 PM and stored at 4C. Juice sac tissue was separated from the rest of the fruit and used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The amplified library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 3 84 well dishes.

text: Mature fruit sample - collected January 2002

characteristic: dev-stage

text: juice sac

text: Citrus sinensis

text: EcoRI

text: XbaI

text: Hermaphrodite

text: Mature Juice Sac from Navel Orange in pTriplEx2 vector

text: Plasmid


text: UC Davis, Department of Pomology

text: live

text: Generic

characteristic: NCBI submission model

text: Washington navel orange

characteristic: lab-host

text: juice sac

text: Citrus sinensis

text: EcoRI

text: XbaI

text: Hermaphrodite

text: Mature Juice Sac from Navel Orange in Vulnus5 vector

text: Plasmid


text: USDA, Agricultural Research Service, Western Regional Research Center

text: live

text: Generic

characteristic: NCBI submission package

text: Kanlow

characteristic: cultivar

text: Callus was established from mature caryopsis of Switchgrass cv. Kanlow and flash frozen in liquid nitrogen. The tissue was cultured by Dan Hayden in the laboratory of CM Tobias. Total RNA and poly(A) RNA were prepared, cDNA synthesized, and directionally ligated into pSPORT1 by Paul Twigg, Biology Department, U. Nebraska Kearney, Kearney, NE. Plasmid DNA preparations and DNA sequencing were performed in the laboratory of CM Tobias.

text: Embryogenic and Nonembryogenic

text: E. coli DH5alpha

text: Callus

text: Panicum virgatum

text: Switchgrass callus cDNA library

text: plasmid


text: live

text: Generic

text: Kanlow

text: Switchgrass cv. Kanlow plants were grown from seed. Young crowns collected from approx 6-8 week old glasshouse grown plants that were starting to tiller. Crowns were cleaned of roots and tillers and flash frozen in liquid nitrogen. The tissue was prepared by Gautam Sarath, USDA, ARS, Wheat Forage and Sorghum Research Unit, U. Nebraska, Lincoln. Total RNA and poly(A) RNA were prepared, cDNA synthesized, and directionally ligated into pSPORT1 by Paul Twigg, Biology Department, U. Nebraska Kearney, Kearney, NE. Plasmid DNA preparations and DNA sequencing were performed in the laboratory of CM Tobias.

text: 6-8 week old plants

text: E. coli DH5alpha

text: Crown

text: Panicum virgatum

text: Switchgrass crown cDNA library

characteristic: title

text: plasmid


text: live

text: Generic

text: Kanlow

text: Switchgrass cv. Kanlow plants were grown from seed in a greenhouse in potting soil. Immature tillers were collected randomly, Leaf tissue was flash frozen in liquid nitrogen. The tissue was prepared by Gautam Sarath, USDA, ARS, Wheat Forage and Sorghum Research Unit, U. Nebraska, Lincoln. Total RNA and poly(A) RNA were prepared, cDNA synthesized, and directionally ligated into pSPORT1 by Paul Twigg, Biology Department, U. Nebraska Kearney, Kearney, NE. Plasmid DNA preparations and DNA sequencing were performed in the laboratory of CM Tobias.

text: immature tillers

text: E. coli DH5alpha

text: Leaf

text: Panicum virgatum

text: Switchgrass leaf cDNA library

text: plasmid


text: live

text: Generic

text: Kanlow

text: Switchgrass cv. Kanlow plants were grown from seed in a greenhouse in potting soil. Flowering tillers were collected and staged to contain an equal number of internodes, the top three internodes below the peduncle were excised and the sheaths removed. Internodes were flash frozen in liquid nitrogen. The tissue was prepared by Gautam Sarath, USDA, ARS, Wheat Forage and Sorghum Research Unit, U. Nebraska, Lincoln. Total RNA and poly(A) RNA were prepared, cDNA synthesized, and directionally ligated into pSPORT1 by Paul Twigg, Biology Department, U. Nebraska Kearney, Kearney, NE. Plasmid DNA preparations and DNA sequencing were performed in the laboratory of CM Tobias.

text: flowering tillers

text: E. coli DH5alpha

text: Internode

text: Panicum virgatum

text: Switchgrass stem cDNA library

text: plasmid


text: The Ohio State University

text: live

text: Generic

text: Normalized cDNA library of J3 (dauer juvenile) stage of entomopathogenic nematode, Heterorhabditis bacteriophora.

text: J3 (dauer stage)

text: Galleria mellonella

text: Heterorhabditis bacteriophora

text: juvenile

characteristic: strain

text: Heterorhabditis bacteriophora Dauer stage cDNA Library

text: plasmid

text: pal16 (evrogen)


text: Laval University

text: live

text: Generic

text: Oocytes

characteristic: cell type

text: T/A cloning with pCR4-TOPO vector from Invitrogen; Library made by Suppressive Subtractive Hybridization from mouse oocytes.

characteristic: description

text: Ovary

text: Mus musculus

text: female

text: Mouse oocyte cDNA subtracted library

text: pCR4-TOPO vector


characteristic: External Id

text: Laval University

text: live

text: Generic

text: Oocytes

text: T/A cloning with pCR4-TOPO vector from Invitrogen; Library made by Suppressive Subtractive Hybridization from Xenopus laevis oocytes.

text: Ovary

text: Xenopus laevis

characteristic: organism

text: female

text: Xenopus laevis oocyte cDNA subtracted library

text: pCR4-TOPO vector


text: China Pharmaceutical University

characteristic: INSDC center name

text: live

text: Generic

text: The cDNA library of the shark regenerated hepatic tissues was constructed with the help of Polymerase Chain Reaction. Total RNA from the regenerated hepatic tissues was separated, and mRNA was purified through the magnetic substance combined with Oligo (dT). The poly-deoxythymidines and poly-deoxycytidines were introduced into the 5'-terminal and 3'-terminal of first cDNA strand with the help of M-MLV reverse transcriptase and terminal transferase respectively. Then double strand cDNA with the restriction site of BamH I and Hind III were synthesized through LD-PCR (Long-distance-PCR) and ligated into the pUC19 vector, recombinant vectors were transformed into E. coli DH5a.

text: Hour 24 after 2/3 partial hepatectomy

text: Escherichia coli DH5a

text: Chiloscyllium plagiosum

text: Hind III

text: hepatic tissues

characteristic: tissue-type

text: cDNA library of Shark hepatic regeneration tissues

text: plasmid


text: Japan Women's University

characteristic: INSDC first public

text: live

text: Generic

text: Nicotiana glauca x Nicotiana langsdorffii library


text: National Institute of Natural Science

text: live

text: Generic

text: Mus musculus

text: head

text: E11 mouse embryo cDNA


text: Research Institute of Innovative Technology for the Earth(RITE)

text: live

text: Generic

characteristic: cell-line

text: Homo sapiens

text: Male

characteristic: sex

text: Kiseru


text: Shinshu University

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: 3'-partial cDNA isolated from primordium by RT-PCR differential display

text: primordium

text: Flammulina velutipes

text: Flammulina velutipes MH092086 primordium


text: Kyoto University

text: live

text: Generic

characteristic: NCBI submission model

text: Bos taurus

text: muscle

text: Bos taurus muscle


text: Wageningen University

text: live

text: Generic

text: preparasitic J2

characteristic: dev-stage

text: Globodera rostochiensis

text: dorsal gland

text: Globodera rostochiensis dorsal gland Ro1 preparasitic J2


text: Wageningen University

text: live

text: Generic

characteristic: NCBI submission package

text: preparasitic J2

text: Globodera rostochiensis

text: subventral gland

text: Globodera rostochiensis subventral gland Ro1 preparasitic J2


text: Universidad de Malaga, Facultad de ciencias

text: live

text: Generic

text: country: Spain

text: Pinus sylvestris

text: cotyledons

text: Pinus sylvestris cotyledons


text: Friedrich-Alexander University Erlangen-Nurnberg

text: live

text: Generic

text: Chevron

characteristic: cultivar

text: Each second spikelet of barley was infected with Fusarium graminearum (strain FG2311, isolated from wheat, 1992, Hohenheim, Germany) by means of micropipette using 5 microliter of conidia suspension (500 conidia /microliter). The infected plants were incubated 4d by 16 h light and 20 degree celcius and infected spikes were incubated the first 48 h by 100 % rel. humidity for establishing of infection. PolyA-RNA was isolated from infected spikes and used to create a cDNA-library using the Creator Lambda TriplEx2 libraries Kit (BD Biosciences). CDNA Fragments were inserted into the vector pLambda-TripleEx2. Subsequently, plasmids were obtained by in vivo excision according to manufacturers instruction. (GABI-Agrotech project)

text: E. coli BM25.8

characteristic: lab-host

text: Hordeum vulgare

text: SfiA (GGCCATTATGGCC)

text: SfiB (GGCCGCCTCGGCC)

text: with Fusarium graminearum infected spikelets

text: GCA

text: pTriplEx2 (BD Biosciences)

characteristic: vector


text: live

text: Generic

text: Chevron

text: Each second spikelet of barley was infected with Fusarium graminearum (strain FG2311, isolated from wheat, 1992, Hohenheim, Germany). by means of micropipette using 5 microliter of conidia suspension (500 conidia/microliter). The infected plants were incubated 4d by 16 h light and 20 Grad Celcius and infected spikes were incubated the first 48 h by 100 % rel. humidity for establishing of infection. PolyA-RNA was isolated from barley spikelets and used to create a cDNA-library by means of the 'SMART cDNA library construction Kit' (CLONTECH). Normalisation was performed essentially as described by Ko (1990, Nucl. Acid Res. 16, 9877) with some modification of Kohchi et al. (1995, Plant J. 8, 771-776). Normalised cDNAs were digested with NotI and cloned into NotI digested pCRblunt vector. (GABI-Agrotech project)

text: E. coli XL1-blue

text: Hordeum vulgare

text: NotI (GCGGCCGC)

text: NotI (GCGGCCGC)

text: GCN

characteristic: title

text: pCR-blunt (Invitrogen)


text: University of Giessen

text: live

text: Generic

text: Ingrid MLG

text: Leaf epidermis; seedlings were grown at 18 degr. C, 60 % rel. humidity, and a photoperiod of 16 h (100 microE * s-1 * m-2). A. 8 days, seedl. were inoculated with Blumeria graminis f.sp. tritici. Leaf epid. was harv. 3, 6, 10, 18, 24, 48 hpi PolyA-RNA was isolated from leaf epidermis and used to create a cDNA-library using the Time Saver cDNA-Synthesis Kit (Amersham). (GABI-Agrotech Project)

characteristic: description

text: Hordeum vulgare

text: leaf epidermis

text: GD

text: pBluescript (Stratagene)


characteristic: External Id

text: live

text: Generic

text: Pasadena

text: Each second spikelet of barley was infected with Fusarium graminearum (strain FG2311, isolated from wheat, 1992, Hohenheim, Germany) by means of micropipette using 5 microliter of conidia suspension (500 conidia /microliter). The infected plants were incubated 4d by 16 h light and 20 degree celsius and infected spikes were incubated the first 48 h by 100 % rel. humidity for establishing of infection. PolyA-RNA was isolated from barley spikelets and used to create a cDNA-library by means of the 'SMART cDNA library construction Kit' (CLONTECH). Normalisation was performed essentially as described by Ko (1990, Nucl. Acid Res. 16, 9877) with some modification of Kohchi et al. (1995, Plant J. 8, 771-776). Normalised cDNAs were digested with NotI and cloned into NotI digested pCRblunt vector. (GABI-Agrotech project)

text: E. coli XL1-blue

text: Hordeum vulgare

text: NotI (GCGGCCGC)

text: NotI (GCGGCCGC)

text: GPN

text: pCR-blunt (Invitrogen)


text: Iowa State University

text: live

text: Generic

text: Uni-ZAP XR vector allows in vivo excision of the pBluescript phagemid. cDNA clones from non acclimated (NA) leaves of Rhododendron catawbiense Michx. RNA for preparation of library was extracted from leaves collected in summer (August) from non acclimated plants.

text: Mature field-grown plants

text: XL1-Blue MRF' E. coli strain

text: Rhododendron catawbiense

characteristic: organism

text: EcoRI (5' end)

text: XhoI (3' end)

text: leave

text: cDNA non acclimated (NA) Rhododendron library

text: Uni-ZAP XR


text: Berry College

characteristic: INSDC center name

text: live

text: Generic

text: Expression induced by 4 hr exposure to 50.0 ug/L naphthalene. Isolated by Differential Display PCR and cloned into Invitrogen pCR II-TOPO cloning vector

text: Adult

text: Acropora cervicornis

text: Coral stress responses

text: pCR II-TOPO


text: live

text: Generic

text: blood leukocytes

characteristic: cell type

text: Bos taurus

text: Bovine peripheral blood leukocytes cDNA subtractive library


text: Forestry and Forest Products Research Institute (FFPRI)

characteristic: INSDC first public

text: live

text: Generic

text: synonym: Populus nigra var. italica

text: juvenile

text: Populus nigra

text: female

text: leaf

characteristic: tissue-type

text: full-length enriched poplar cDNA library


text: Shanghai Univ.

text: live

text: Generic

text: Capsicum annuum

text: placenta

text: Capsicum annuum placenta


text: Nara Institute of Science and Technology

text: live

text: Generic

characteristic: cell-line

text: Bright Yellow No.2

text: Nicotiana tabacum

text: RIKEN, BY-2 cDNA library


text: University of Texas

characteristic: INSDC last update

text: live

text: Generic

text: Mus musculus

characteristic: strain

text: spleen

text: Mus musculus spleen C57BL/6


text: University of Texas

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Homo sapiens

text: tonsil

text: Homo sapiens tonsil


text: Iowa State University

text: live

text: Generic

characteristic: NCBI submission package

text: Inbred B73

text: This library was constructed by Xiaolan Zhang. Vegetative Shoot Apical Meristem (SAM) and leaf primordia staged P1-P4 from 14-17 day-after germination seedlings were quickly dissected into dry ice under a light microscope. Total RNA was isolated using Trizol and mRNA was purified with Dynal Oligo-DT25. ds-cDNA molecules were generated as follows. First-strand cDNA was prepared from oligo-dT selected mRNA by priming with an XhoI oligo-dT primer (5'-GAGAGAGAGAGAGAGAGAGAACTAGTCTCGAGTTTTTTTTTTTTTTTTTT). The resulting DNA:RNA hybrid was treated with RNase H and used as a template for DNA PolI-catalyzed second strand synthesis. After the addition of EcoRI adaptors, the ds-cDNAs were digested with XhoI and size-selected from 300 bp to 600 bp. The resulting molecules were directionally cloned into the EcoRI and XhoI sites of the Uni-Zap XR vector. The lambda library was packaged with Gigapack III Gold packaging extract and was mass excised by XL1-Blue cells and ExAssist Helper phage. Excised phagemids were titered in SOLR cells and plated onto LB-ampicillin agar plates. Base calling was conducted using Phred. Trimming was performed using Lucy with the following criteria: (-minimum 200 -error 0.01 0.01 -bracket 10 0.01). A low complexity filter was applied and additional trimming was conducted to remove E. coli, vector, and organelle contamination. After processing ~30% of the sequences contained a minimum of 10 Ts at the beginning of the sequence. For reasons that are not understood many of the clones in this library lack an XhoI site at their 3' ends.

text: Shoot apex

characteristic: organ

text: Zea mays

text: EcoRI

text: XhoI

text: Uni-Zap XR


text: Huazhong Agricultural University of China

text: live

text: Generic

text: inbred Mo17

characteristic: cultivar

text: mRNA was isolated from roots of seedlings with three leaves treated by water-logging (including roots) for CK, 12h, 16h, 20h, 24h. SSH libraries were made according to CLONTECK PCR-Select cDNA Subtraction Kit (Clontech,USA). The treated sample as the driver was combined of the mRNA from 12h, 16h, 20h, 24h , while the CK sample was treated as the tester.

text: three leaves period

characteristic: dev-stage

characteristic: lab-host

text: root

text: Zea mays

text: hermaphrodite

characteristic: sex

text: root

text: Zea Mays inbred line Mo17 late hypoxia-induced reverse SSH library

text: Plasmid

text: pGEM T-vector


characteristic: External Id

text: Chang Gung University

text: live

text: Generic

text: Angiostrongylus cantonensis fifth stage larvae were obtained from the brain of laboratory infected SD rats. Directional cDNA was synthesized by oligo d(T) priming and cloned into the EcoRI/Xho sites of the lambda ZAP vector by using the ZAP-cDNA synthesis kit (Stratagene).

characteristic: description

text: Fifth stage larva

text: XL1 Blue-MRF'

text: Angiostrongylus cantonensis

text: EcoRI

text: XhoI

text: male and female

text: Taiwan strain

text: AcL5 cDNA Library

characteristic: title

text: Lambda ZAP-Express (Stratagene)

characteristic: vector


text: USDA-ARS

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: A high quality EST with at least 200 contiguous bases at Trace Tuner score of 20 or better. Construction by Laura Hunnicutt,USDA,ARS, U.S. Horticultural Res.Lab, Ft.Pierce, FL, USA.

text: 5th instar

text: Homalodisca vitripennis

characteristic: organism

text: EcoRI

text: XhoI

text: Mixed population

text: whole body

text: 5th Instar Glassy-winged Sharpshooter

text: pBluescript II SK+


text: USDA-ARS

characteristic: INSDC last update

text: live

text: Generic

text: Source: Field collected June 2004, Puerto Rico. A high quality EST with at least 200 contiguous bases at Trace Tuner score of 20 or better. Library construction by PM. DANG, USDA,ARS, U.S. Horticultural Res. Lab, Ft. Pierce, FL, USA.

text: Adults

text: Diaprepes abbreviatus

text: EcoRI

text: XhoI

text: Mixed population

text: Whole body

characteristic: tissue-type

text: Citrus root weevil whole body adult

text: pBluescript II SK+


text: USDA-ARS

text: live

characteristic: INSDC status

text: Generic

text: Source: Field collected from citrus, June 2003, Port St. Lucie, FL. A high quality EST with at least 200 contiguous bases at Trace Tuner score of 20 or better. Library construction by PM. DANG, USDA, ARS, U.S. Horticultural Res. Lab, Ft.Pierce, FL, USA.

text: nymphs - adults

text: Diaphorina citri

text: EcoRI

text: XhoI

text: Mixed population

text: whole body

text: Asian citrus psyllid

text: pBluescript II SK+


text: University of Minnesota

text: live

text: Generic

characteristic: NCBI submission model

text: Differential display of cranial and trunk neural crest. cDNA fragments cloned into the pGEM-T vector.

text: Gallus gallus

text: Neural crest

text: Cranial/trunk neural crest differential display

text: pGEM-T Vector


text: The Second hospital of Tianjin Medical University

text: live

text: Generic

characteristic: NCBI submission package

text: Total RNA were isolated from the tissues of microdissected bladder transitional cell carcinoma and corresponding normal bladder mucosa respectively. Then double-strand cDNA were synthesized by cDNA SMART technology and restricted by Rsa I. The carcinoma cDNA were divided into two groups and ligated with either adaptor 1 or adaptor 2. After hybridizing with normal cDNA twice and undergoing nested PCR, the PCR products were cloned into PT-Adv vector and transformed E.Coli TOP10F

text: bladder

text: Homo sapiens

text: Ras

text: Ras

text: male

text: bladder cancer

text: subtractive library of high grade bladder transitional cell carcinoma

text: Plasmid

text: PT-Adv


text: Zhejiang Sci-Tech University

text: live

text: Generic

text: The synthesis of double-stranded cDNA from mRNA was based on the method described by Gubler and Hoffman. The obtained cDNA were ligated into the plasmid vector pHelix and subsequently the ligation product was tranformed into E.coli competent cells TG1. At last, the recombinant clones were screened by blue-white plaques.

text: metaphase

text: Bombyx mori

text: Hind II

text: male and female

text: Qingsong-Haoyue

characteristic: strain

text: whole pupae body but for the skin

text: BmP

text: Plasmid

text: pHelix


text: Shantou University Medical College

text: live

text: Generic

text: neuroblastoma cell line SK-N-SH

characteristic: cell-line

text: Homo sapiens

text: Human Neuroblastoma Cell Line SK-N-SH Express Library

text: pGEM-T Easy


text: Centre International de Recherche Agronomique pour le Developpement

text: live

text: Generic

text: embryonic

characteristic: dev-stage

text: Theobroma cacao

text: embryo

text: Theobroma cacao somatic embryo RNA


text: live

text: Generic

text: embryonic

text: Theobroma cacao

text: embryo

text: Theobroma cacao immature zygotic embryo RNA


text: live

text: Generic

text: embryonic

text: Theobroma cacao

text: embryo

text: Theobroma cacao mature zygotic embryo RNA


text: Embrapa Recursos Geneticos e Biotecnologia

text: live

text: Generic

text: Variety Calcutta 4 (AA)

characteristic: cultivar

text: In vitro micro propagated M. acuminata ssp. burmannicoides var. Calcutta 4 (AA) plants, 30 cm high, were used in this study. These plants were obtained from the Banana Germplasm Bank at Embrapa Cassava and Tropical Fruits, Cruz das Almas, Bahia, Brazil (Musa Germplasm Information System - MGIS accession number ITC0249). Prior to temperature stress conditions, banana plants were maintained for 48 hours in an environment chamber at 25oC with 80% air humidity, and a 16 hour light/ 8 hour dark cycle. Leaf samples (H0) were removed from plants at 25oC then, following a temperature rise to 35oC, samples were collected after one (H1), two (H2) and three (H3) hours. With the temperature raised to 45oC, new samples were also collected after one (H4), two (H5), three (H6), nine (H7), and 21 hours (H8) following the change in temperature. Tissues were immediately frozen in liquid N and stored at -80oC until total RNA extraction. Total RNA was isolated using the Plant RNA Reagent kit (Invitrogen). Total RNA preparations from the eight time point samples were combined in equivalent quantities. The resulting bulked sample (1 mg of total RNA) was then submitted to poly (A) + RNA purification using the FastTrack 2.0 mRNA Isolation Kit (Invitrogen). Full length cDNA enriched, non-normalized, cDNA libraries were constructed with the Creator Smart cDNA library kit and cloned into the pDNR-LIB vector (Clontech).

text: Musa acuminata subsp. burmannicoides

text: Leaf

text: Musa acuminata leaves HOT stress

text: pDNR-LIB (Clontech)


text: live

text: Generic

text: Variety Calcutta 4 (AA)

text: In vitro micro propagated M. acuminata ssp. burmannicoides var. Calcutta 4 (AA) plants, 30 cm high, were used in this study. These plants were obtained from the Banana Germplasm Bank at Embrapa Cassava and Tropical Fruits, Cruz das Almas, Bahia, Brazil (Musa Germplasm Information System - MGIS accession number ITC0249). Prior to temperature stress conditions, banana plants were maintained for 48 hours in an environment chamber at 25oC with 80% air humidity, and a 16 hour light/ 8 hour dark cycle. Leaf samples (C0) were collected from plants at 25oC then, with the temperature decreased to 15oC, samples were collected one (C1), two (C2) and three (C3) hours after the temperature change. With the temperature decreased to 5oC, new samples were collected after one (C4), two (C5), three (C6), nine (C7) and 21 hours (C8) after the change in temperature. Tissues were immediately frozen in liquid N and stored at -80oC until total RNA extraction. Total RNA was isolated using the Plant RNA Reagent kit (Invitrogen). Total RNA preparations from the eight time point samples were combined in equivalent quantities. The resulting bulked sample (1 mg of total RNA) was then submitted to poly (A) + RNA purification using the FastTrack 2.0 mRNA Isolation Kit (Invitrogen). Full length cDNA enriched, non-normalized, cDNA libraries were constructed with the Creator Smart cDNA library kit and cloned into the pDNR-LIB vector (Clontech).

text: Musa acuminata subsp. burmannicoides

text: Leaf

text: Musa acuminata leaves COLD stress

text: pDNR-LIB (Clontech)


text: National Taiwan University

text: live

text: Generic

text: 2-year-old Taiwania seedlings were obtained from the Experimental Forest of National Taiwan University. The leaves were reomved and the developing stem tissues were frozen in liquid nitrogen. The cDNA library was constructed by pGEM-T Easy vector system (Promega).

text: Taiwania cryptomerioides

text: developing stem tissue of seedling

text: Taiwania developing stem tissue cDNA library

text: pGEM-T Easy


text: Institute for Molecular Biotechnology

text: live

text: Generic

text: Samples for total RNA isolation were taken from a continous culture of Mesostigma viride Lauterborn (strain NIES476). mRNA was isolatede using the mTRAP kit (Active Motif). Using the Superscript plasmid system the mRNA was converted to cDNA. After column fractionation the cDNAs were cloned into pSPORT. DSN normalized fractions were generated from SMART purified RNA samples and cloned into pGEM-TEasy.

text: Mesostigma viride

text: Mesostigma vegetative library


text: University of Illinois at Urbana-Champaign

text: live

text: Generic

text: Normalized/Subtracted cDNA Library. Library contains a tag sequence (5'-TGCGA-3') after the poly(A) tail. The EcoRI restriction site was added with the adaptor 5'-AATTCCATTGTGTTGGG-3'

text: Reproductively mature adult

text: Schmidtea mediterranea

text: EcoRI

text: NotI

text: Hermaphrodite

characteristic: sex

text: Sexual clone CIWsx2

text: cDNA from sexually mature hermaphodites

text: Plasmid

text: pBS SKII(+)


characteristic: External Id

text: live

text: Generic

text: Normalized/Subtracted cDNA Library. Library contains a tag sequence (5'-TGCGA-3') after the poly(A) tail. The EcoRI restriction site was added with the adaptor 5'-AATTCCGTTGCTGTCG-3'

text: Reproductively immature

text: Schmidtea mediterranea

text: EcoRI

text: NotI

text: Hermaphrodite

text: Sexual clone CIWsx2

text: cDNA from juvenile hermaphodites

text: Plasmid

text: pBS SKII(+)


text: USDA-ARS

text: live

text: Generic

text: source: Colonies maintained by Dr. McKenzie, USHRL, Fort Pierce, FL. A high quality EST with at least 200 contiguous bases at Trace Tuner score of 20 or better. Library construction by Laura Hunnicutt, USDA, ARS, U.S. Horticultural Res. Lab, Ft.Pierce, FL, USA.

characteristic: description

text: adults

characteristic: lab-host

text: Diaphorina citri

text: EcoRI

text: XhoI

text: Mixed genders

text: whole body

text: Diaphorina citri Kuwayama (Hemiptera: Psylloidea)

text: pBluescript II SK+


text: USDA-ARS

text: live

text: Generic

text: A high quality EST with at least 200 contiguous bases at Trace Tuner score of 20 or better. Library construction by Laura Hunnicutt, USDA,ARS, U.S. Horticultural Res.Lab, Ft.Pierce, FL, USA.

text: Adult

text: Oncometopia nigricans

text: EcoRI

text: XhoI

text: Mixed

text: whole body

text: Adult sharpshooter leafhopper

characteristic: title

text: pBluescript II SK+

characteristic: vector


text: Center for Integrated Fungal Research, NC State University

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: Mycelial culture grown under conditions of Nitrogen Starvation.

text: mycelia

text: Alternaria brassicicola

text: ATCC 96866

text: A. brassicicola mycelial culture grown under conditions of Nitrogen Starvation

text: Plasmid

text: pGEMt


characteristic: INSDC last update

text: live

text: Generic

text: Mycelial culture infecting Brassica napus (Non-Directional Library Clone)

text: mycelia

text: Alternaria brassicicola

characteristic: organism

text: ATCC 96866

text: A. brassicicola mycelial culture infecting B. napus

text: Plasmid

text: pGEMt


text: live

characteristic: INSDC status

text: Generic

text: Mycelia Culture grown for 48 hours, 24 degrees C, minimal media minus Nitrogen. Infecting Brassica oleracea Var. Capitata cultivar 'tendersweetF1'

text: mycelia

text: Alternaria brassicicola

text: ATCC 96866

text: A. brassicicola mycelial culture infecting B. oleracea

text: Plasmid

text: pGEMt


text: Cornell University

text: live

text: Generic

characteristic: NCBI submission model

text: accession 30-5

text: 3-weeks old seedling leaf

text: E. coli DH5a

text: Eragrostis pilosa

text: Not I [non-directional cloning]

text: leaf

characteristic: tissue-type

text: E. pilosa (wild type of tef) leaf

text: Plasmid

text: pPCR-Script Cam SK (+)


text: Cornell University

text: live

text: Generic

characteristic: NCBI submission package

text: Kaye Murri

text: 5-15 DAP

text: E. coli DH5a

text: Eragrostis tef

text: Not I [non-directional cloning]

text: inflorescence spike

text: tef (Kaye Murri) flower

text: Plasmid

text: pPCR-Script Cam SK (+)


text: Cornell University

text: live

text: Generic

text: Kaye Murri

text: 3-weeks old seedling leaf

text: E. coli DH5a

text: Eragrostis tef

text: Not I [non-directional cloning]

text: Xho I

text: leaf

text: tef (Kaye Murri) leaf

text: Plasmid

text: pPCR-Script Cam SK (+)


text: Cornell University

text: live

text: Generic

text: Kaye Murri

text: 3-weeks old seedling

text: E. coli DH5a

text: Eragrostis tef

text: Not I [non-directional cloning]

text: root

text: tef (Kaye Murri) root

text: Plasmid

text: pPCR-Script Cam SK (+)


text: Umea University

text: live

text: Generic

text: Populus trichocarpa

text: Populus cDNA, trichocarpa shoot library


text: Cornell University

text: live

text: Generic

text: Unstimulated [Tag-(polyA)TCGGA] & stimulated in vitro with PWM [Tag-(polyA)TGCGA]

text: DH12S E. coli cells (Invitrogen)

text: Equus caballus

text: Thoroughbred & Standardbred breeds

characteristic: strain

text: Lymphocytes

text: Equine lymphocyte cDNA Library

text: Plasmid

text: pGEM11Zf(+) (Promega)


text: Cornell University

text: live

text: Generic

text: Pooled day 34 equine conceptuses

characteristic: dev-stage

text: DH12S E. coli cells (Invitrogen)

text: Equus caballus

text: Thoroughbred, Standardbred, & Pony breeds

text: Invasive [Chorionic Girdle (Tag-(polyA)TGCGT)] and Non-invasive [Allantochorion (Tag-(polyA)TCGGT)] trophoblast

text: Equine placenta cDNA Library

text: Plasmid

text: pGEM11Zf(+) (Promega)


text: Swedish University of Agricultural Sciences (SLU)

text: live

text: Generic

text: 36 hours after inoculation with downy mildew Sclerospora graminicola

text: seedling

text: Cenchrus americanus

text: Pennisetum glaucum seedling


text: Lethbridge Research Centre, Agriculture and Agri-Food Canada

text: live

text: Generic

text: Neepawa and its near-isogenic sib BW553

characteristic: cultivar

text: Triticum aestivum

text: seedling crown tissue

text: Triticum aestivum SSH cDNA library

text: plasmid

text: pGEM-T


text: University of Georgia

text: live

text: Generic

text: Hitchcock

text: Sorghum propinquum

text: pSPR

text: Plasmid


text: Institute of Hydrobiology, Chinese Academy of Sciences

text: live

text: Generic

text: Epinephelus coioides

text: male

characteristic: sex

text: hypothalamus

text: orange-spotted grouper hypothalamus cDNA library


characteristic: External Id

text: Norwegian Forest Research Institute

text: live

text: Generic

text: Libraries were constructed by means of suppressive subtraction hybridization (SSH) technique using Clontech PCR-Select cDNA Subtraction Kit (Clontech, # K1804-1). Obtained arrays were non-directionally cloned in pDrive vector using QIAGEN PCR Cloning Kit (#231224).

text: 4 weeks before bud burst

text: Twigs

characteristic: organ

text: Picea abies

text: Dormant buds

text: Late subtracted library

text: pDrive


text: SCPFRC

text: live

text: Generic

text: Baby gold 5

text: Leaf tissue of Prunus persica L. infected by Plum pox virus

characteristic: description

text: Prunus persica

text: Leaf

text: Prunus persica L. leaf tissue, Plum Pox Virus infected


text: SCPFRC

text: live

text: Generic

text: Baby gold 5

text: Leaf tissue of Prunus persica L. not infected by Plum pox virus

text: Prunus persica

text: Leaf

text: Prunus persica L. leaf tissue, healthy

characteristic: title


text: ICAPB

text: live

text: Generic

text: Litomosoides sigmodontis

text: Female

text: Litomosoides sigmodontis adult female 1 (high molecular weight)


text: UMR879 INRA-CNRS-ENSL-UCBL

text: live

text: Generic

text: Sample name: EMB7 ; Plant growth place: growth chamber ENS-Lyon ; Soil conditions: inert substrate ; Sowing date: 01/04/1993 ; Harvesting date: 26/06/1993 ; Description: micro-dissected embryos ; experimental condition: normal

text: 7 days after pollination

text: SOLR

characteristic: lab-host

text: Zea mays

text: EcoRI

text: XhoI

text: embryo

text: pBluescript II SK(+)

characteristic: vector


text: Baylor College of Medicine

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: primary mesenchyme cells

characteristic: cell type

text: oligo dt priming from poly A+ RNA, directionally cloned

text: E.coli

text: Strongylocentrotus purpuratus

text: NotI

text: MluI

text: embryo

text: Sea Urchin primary mesenchyme cell cDNA library


text: The Canadian Potato Genome Project - BioAtlantech

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Shepody

text: supplier: Pathogen-challenge series. Tubers from pathogen-free Solanum tuberosum var. Shepody, clone 1756, were inoculated with 5 ul of Phytophthora infestans (A2-mating type), through 2 puncture wounds 3 cm apart. The tubers were incubated 1 minute to allow inoculum absorption. The infection area was outlined for future collection reference. Surface slices were sampled from the tubers at 1 day, 5 days, 7 days, 11 days and 14 days post-infection. All samples were pooled and used for RNA isolation and library construction. A normalized library was constructed following a modified protocol of Bonaldo et al. (1996. Genome Research 6: 791-806).

text: Solanum tuberosum

characteristic: organism

text: EcoRI

text: XhoI

text: Tubers

text: Late Blight-Challenged Tubers

text: pBluescript II SK(+) XR


text: Universitaet Hamburg, Biozentrum Klein Flottbek

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: egg cell

text: SMART cDNA synthesis and PCR-Select cDNA subtraction (Clontech) of egg cell cDNA with central cell cDNA, PCR products T/A cloned

text: mature

text: E. coli, XL1 Blue

text: Zea mays

text: Subtracted egg cell cDNA library

text: Plasmid

text: pGEM T-Easy, Promega


text: live

text: Generic

text: central cell

text: SMART cDNA synthesis and PCR-Select cDNA subtraction (Clontech) of central cell cDNA with egg cell cDNA, PCR products T/A cloned

text: mature

characteristic: dev-stage

text: E. coli, XL1 Blue

text: Zea mays

text: Subtracted central cell cDNA library

text: Plasmid

text: pGEM T-Easy, Promega


text: Washington State University

text: live

text: Generic

text: Morex

characteristic: cultivar

text: Hordeum vulgare subsp. vulgare

text: ovary

characteristic: tissue-type

text: Hordeum vulgare subsp. vulgare ovary


characteristic: External Id

text: Southeast Missouri State University

text: live

text: Generic

text: Fruit body caps harvested from one hour before to one hour after karyogamy (nuclear fusion)

text: XL-1 Blue

text: Coprinopsis cinerea

text: EcoRI

text: XhoI

text: Dikaryon - strains backcrossed into Okayama7#130 thru 5 gens

characteristic: strain

text: Coprinus cinereus fruit body cap cDNAs in early meiotic prophase (K-1 to K+1)

text: phagemid

text: pBluescript II SK-


text: Southeast Missouri State University

text: live

text: Generic

text: Fruit body caps harvested six hours after karyogamy (nuclear fusion)

text: E. coli DH10B

text: Coprinopsis cinerea

text: EcoRI

text: XhoI

text: Coprinus cinereus fruit body cap cDNAs in meiotic prophase (K+6)

text: phagemid

text: pAD-Gal 4-2.1


text: Southeast Missouri State University

text: live

text: Generic

text: vegetative monokaryotic mycelium

text: E. coli XL10-Gold

text: Coprinopsis cinerea

text: EcoRI

text: XhoI

text: Coprinus cinereus rapamycin-treated mycelia cDNAs

text: phagemid

text: pBluescript II SK+


text: Southeast Missouri State University

text: live

text: Generic

text: vegetative monokaryotic mycelium

text: E. coli XL10-Gold

text: Coprinopsis cinerea

text: EcoRI

text: XhoI

text: Coprinus cinereus minimal medium exponentially growing mycelia cDNAs

text: phagemid

text: pBluescript II SK+


text: Beijing Genomics Institute

text: live

text: Generic

text: Sus scrofa

text: Sus scrofa Expressed Library


text: Meiji University

text: live

text: Generic

text: adult

text: Anas platyrhynchos

text: female

characteristic: sex

text: brain

text: full-length duck cDNA library


text: Miyazaki University

text: live

text: Generic

text: Seriola quinqueradiata

text: head kidney

text: Seriola quinqueradiata head kidney

characteristic: title


text: Korea Research Institute of Bioscience and Biotechnology

text: live

text: Generic

text: Bugang

text: bacterial-induced

characteristic: description

text: Capsicum annuum

text: nonhost-resistance related DDRT-PCR fragments


text: University of Delhi, South Campus

text: live

text: Generic

text: Oryza sativa

text: Oryza sativa library (Agarwal S)


text: University of Zurich

text: live

text: Generic

text: country: Switzerland; forma_specialis: hordei.

text: Blumeria hordei

text: Blumeria graminis f. sp. hordei Switzerland library


text: live

text: Generic

text: TDFs (transcriptionally derived fragments) from cDNA-AFLP were isolated, sequenced and cloned

text: stem

characteristic: organ

text: Eucalyptus grandis x Eucalyptus nitens

text: hermaphrodite

text: mature xylem, immature xylem, phloem, cork

text: Eucalyptus woody tissue TDF collection


text: National University of Singapore

characteristic: INSDC center name

text: live

text: Generic

text: Pluripotent human embryonic stem cell line

text: HES-3 (XX)

characteristic: cell-line

text: Homo sapiens

text: HES3 rSAGE


text: Gyeongsang National University

characteristic: INSDC first public

text: live

text: Generic

text: Columbia

text: To clone DNA sequences for functional analysis and expression in multiple systems

text: E. coli

characteristic: lab-host

text: Arabidopsis thaliana

text: Arabidopsis Gateway cDNA Library

text: Plasmid

characteristic: vector


text: Gyeongsang National University

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Columbia

text: 4 week-old plants

text: E. coli

text: Arabidopsis thaliana

characteristic: organism

text: BamHI

text: SmaI

text: Arabidopsis CaMs library

text: Plasmid

text: pGEM-T Easy


text: Dipartimento di Biologia Vegetale, Universita degli Studi di Torino

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: cDNA library of Tuber borchii vegetative mycelium grown on PDA medium

text: vegetative hyphae

characteristic: dev-stage

text: Tuber borchii

text: mycelium

text: Tuber borchii vegetative mycelium grown on PDA library

text: Phagemid (Stratagene,USA)

text: pBK-CMV phagemid


characteristic: External Id

text: Beijing Genomics Institute, CAS (Hu); Institute of oceanology, CAS (Wang)

text: live

text: Generic

text: Sporophyte

text: Neoporphyra haitanensis

text: Sporophyte cDNA Library

text: phagemid

text: pBluescriptII


text: UMR INRA 1088 Plante-Microbe-Environment

text: live

text: Generic

characteristic: cultivar

text: variety: gaertn

text: Medicago truncatula

text: root

characteristic: tissue-type

text: Medicago truncatula cv. J5 root


text: The Ohio State University-OARDC

text: live

text: Generic

text: Grown on GYM medium for 29 days till media was almost depleted to mimic stress conditions

characteristic: isolate

text: Saprolegnia parasitica

text: Sal1 (G/TCGAC)

text: Not1 (GC/GGCCGC)

text: mycelium

text: Saprolegnia parasitica ATCC90214 mycelium library

characteristic: title

text: Plasmid