text: Biozentrum der Universitaet Basel

text: live

text: Generic

text: Drosophila melanogaster

text: line B1350

text: Drosophila melanogaster line B1350


status: PUBLIC

characteristic: External Id

text: Biozentrum der Universitaet Basel

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Drosophila melanogaster

characteristic: organism

text: line B0729

tag: attribute

characteristic: strain

text: Drosophila melanogaster line B0729

characteristic: title


text: Biozentrum der Universitaet Basel

text: live

text: Generic

text: Drosophila melanogaster

text: line B0748

text: Drosophila melanogaster line B0748


text: Biozentrum der Universitaet Basel

text: live

text: Generic

text: Drosophila melanogaster

text: line B2038

text: Drosophila melanogaster line B2038


text: Biozentrum der Universitaet Basel

text: live

text: Generic

text: Drosophila melanogaster

text: line B0942

text: Drosophila melanogaster line B0942


text: Biozentrum der Universitaet Basel

text: live

text: Generic

text: Drosophila melanogaster

text: line B0404

text: Drosophila melanogaster line B0404


text: Biozentrum der Universitaet Basel

text: live

text: Generic

text: Drosophila melanogaster

text: line B1522

text: Drosophila melanogaster line B1522


text: Biozentrum der Universitaet Basel

text: live

text: Generic

text: Drosophila melanogaster

text: line B0674

text: Drosophila melanogaster line B0674


text: Biozentrum der Universitaet Basel

text: live

text: Generic

text: Drosophila melanogaster

text: line B0975

text: Drosophila melanogaster line B0975


text: Biozentrum der Universitaet Basel

text: live

text: Generic

text: Drosophila melanogaster

text: line B2149

text: Drosophila melanogaster line B2149


text: Biozentrum der Universitaet Basel

text: live

text: Generic

text: Drosophila melanogaster

text: line B1501

text: Drosophila melanogaster line B1501


text: Biozentrum der Universitaet Basel

text: live

text: Generic

text: Drosophila melanogaster

text: line B0079

text: Drosophila melanogaster line B0079


text: Biozentrum der Universitaet Basel

text: live

text: Generic

text: Drosophila melanogaster

text: line B2033

text: Drosophila melanogaster line B2033


text: Biozentrum der Universitaet Basel

text: live

text: Generic

text: Drosophila melanogaster

text: line B2036

text: Drosophila melanogaster line B2036


text: Biozentrum der Universitaet Basel

text: live

text: Generic

text: Drosophila melanogaster

text: line B0929.3'P

text: Drosophila melanogaster line B0929.3'P


text: Biozentrum der Universitaet Basel

text: live

text: Generic

text: Drosophila melanogaster

text: line B0929.5'P

text: Drosophila melanogaster line B0929.5'P


text: Biozentrum der Universitaet Basel

text: live

text: Generic

text: Drosophila melanogaster

text: line B0960

text: Drosophila melanogaster line B0960


text: University of Geneva Medical School

text: live

text: Generic

text: direct sequencing of PCR product; sequences orthologous to human chromosome 21 conserved sequences or non-coding RNAs from the whole genome

characteristic: description

text: Chlorocebus aethiops

text: Green monkey genomic DNA


text: University of Agricultural Sciences (UAS), GKVK, and Bangalore

text: live

text: Generic

characteristic: cultivar

text: Genomic DNA was isolated from 4 week-old healthy mulberry leaves, and PCR amplified using specific primer. The amplified product was cloned into T/A cloning vector pTZ57R/T

text: 4 week old leaves

characteristic: dev-stage

text: Escherichia coli

characteristic: lab-host

text: Leaf

characteristic: organ

text: Morus alba

text: ECOR I

text: Hind III

text: Bisexual

characteristic: sex

text: Leaf tissue

characteristic: tissue-type

text: Genomic DNA of mulberry

characteristic: vector


characteristic: External Id

text: University of Canberra

characteristic: INSDC center name

text: live

text: Generic

text: DNA was extracted from blood of an Australian central bearded dragon. AFLP fragment

text: Pogona vitticeps

text: blood

text: Pogona vitticeps AFLP fragment


text: Massachusetts Institute of Technology

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Library prepared from total DNA extracted from picoplankton sample. Environmental sample, Monterey Bay California, Station M1, 100 meters depth.

text: E. coli DH5alpha, Epi300

text: uncultured Nitrospinaceae bacterium

text: Fosmid


text: Massachusetts Institute of Technology

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Library prepared from total DNA extracted from picoplankton sample. Environmental sample, North Pacific Subtropical Gyre, 200 meters depth.

text: E. coli DH5alpha, Epi300

text: uncultured Nitrospinaceae bacterium

text: Fosmid


text: Massachusetts Institute of Technology

text: live

text: Generic

characteristic: NCBI submission package

text: Library prepared from total DNA extracted from picoplankton sample. Environmental sample, North Pacific Subtropical Gyre, 4000 meters depth.

text: E. coli DH5alpha, Epi300

text: uncultured Nitrospinaceae bacterium

characteristic: organism

text: Fosmid


text: Massachusetts Institute of Technology

text: live

text: Generic

text: Library prepared from total DNA extracted from picoplankton sample. Environmental sample, North Pacific Subtropical Gyre, 500 meters depth.

text: E. coli DH5alpha, Epi300

text: uncultured Nitrospinaceae bacterium

characteristic: title

text: Fosmid


text: Massachusetts Institute of Technology

text: live

text: Generic

text: Library prepared from total DNA extracted from picoplankton sample. Environmental sample, North Pacific Subtropical Gyre, 770 meters depth.

characteristic: description

text: E. coli DH5alpha, Epi300

text: uncultured Nitrospinaceae bacterium

text: Fosmid


text: Howard Hughes Medical Institute at University of Washington

text: live

text: Generic

text: Gorilla gorilla

text: female

text: skin

text: Gorilla gorilla NG05251 Coriell Inst NIA Aging Cell


text: live

text: Generic

text: Macaca mulatta

text: female

text: skin

text: Macaca mulatta NG07109 Coriell Inst NIA Aging Cell


text: live

text: Generic

text: Pan paniscus

text: male

text: skin

text: Pan paniscus NG05253 Coriell Inst NIA Aging Cell


text: live

text: Generic

text: Pongo pygmaeus

text: female

text: skin

text: Pongo pygmaeus NG12256 Coriell Inst NIA Aging Cell


text: live

text: Generic

text: Pan troglodytes

text: male

text: skin

text: Pan troglodytes NG06939 Coriell Inst NIA Aging Cell


text: Melaka Institute of Biotechnology

text: live

text: Generic

text: Somatic

characteristic: cell type

text: Genomic DNA (10 ug) of Elaeis oleifera was digested with EcoRI restriction enzyme, and generated EcoRI fragments were cloned into pGEM-T Easy cloning vector [predigested with EcoRI]. Cloning of the generated and purified EcoRI fragments (of Eo gDNA) and selection of the recombinant clones was completed following guidelines given by the pGEM-T Easy vector system supplier (Promega). Ampicillin resistant recombinant colonies were selected from plates for pDNA isolation (blue/white colony selection method). Plasmid DNA was isolated and purified using Wizard Plus SV minipreps DNA purification system (Promega).

text: Young

text: Elaeis oleifera

text: Leaf

text: Oil palm Eo leaf-tissue gDNA-Library (pGEM-T Easy)


text: Melaka Institute of Biotechnology

text: live

text: Generic

text: Somatic

text: Genomic DNA (10 ug) of Elaeis oleifera was digested separately with NdeI and DraI restriction enzymes. NdeI restriction enzyme digestion generated genomic DNA fragments were made blunt-ended using T4 DNA polymerase enzyme (Promega). DNA fragments generated by both NdeI and DraI restriction enzymes digestion were purified, pooled together and cloned using components from CloneMiner cDNA Library Construction Kit procured from local distributor of Invitrogen Corporation.

text: Young

characteristic: dev-stage

text: Elaeis oleifera

text: Leaf

text: Oil palm Eo leaf-tissue gDNA Entry Library(CloneMiner)


text: University of Milan

text: live

text: Generic

text: Gorilla gorilla

text: EcoRI

text: EcoRI

text: male

text: CHORI-255 (Gorilla gorilla) BAC Library

text: BAC


text: Stanford Human Genome Center

text: live

text: Generic

characteristic: cultivar

text: DH10B (T1 phage resistant)

characteristic: lab-host

text: Sorghum bicolor

text: BamHI

text: BamHI

text: young seedlings

text: BAC


text: 1. University of California, Davis and 2. The Institute for Genomic Research

text: live

text: Generic

text: Blackeye 5

text: Seed of cowpea (Vigna unguiculata) cultivar Blackeye 5 was germinated in sterile soil under greenhouse conditions for 3-4 days. As the seedling hook was about to break ground, the pots were covered to exclude almost all light for approximately 36 hours. The yellow-green primary leaves were harvested and snap-frozen under liquid nitrogen. Nuclei were isolated in a high pH Tris buffer to counteract tissue acidity. The nuclei were embedded in low melting point agarose, digested with proteinase K, restriction digested with Hind III and size selected by means of two rounds of pulsed field gel electrophoresis. Large size DNA fragments were ligated into HindIII-cut vector pCC1BACH and transformed by electroporation in to E. coli Transformax EPI300 (Epicentre) cells. Approximately 50 clones were assessed for insert size by excision of the insert by NotI digestion and CHEF gel analysis of the products. Most inserts were 40-90 kbp with an average insert size of 65 kbp. 73,728 clones were collected and BAC end sequences were obtained for ~2300 clones.

text: Shaded primary leaves

characteristic: organ

text: Vigna unguiculata

text: HindIII

text: VUUBBa (VUH1)

text: Epicenter Copy Control pCC1BAC


text: live

text: Generic

text: Blackeye 5 line 9405C

text: Cowpea (Vigna unguiculata) cultivar Blackeye 5 was crossed to cowpea line Arlington, the source of the dominant Cpa gene for resistance to Cowpea mosaic virus. Blackeye 5 was the recurrent parent in an eight-fold backcross initiated with the Blackeye 5 x Arlington cross. 9405C is a line (genotype Cpa/Cpa) derived from several generations of selfing from a single seed derived from the backcross series. 9405C seeds were germinated in sterile soil under greenhouse conditions for 3-4 days. As the seedling hook was about to break ground, the pots were covered to exclude almost all light for approximately 36 hours. The yellow-green primary leaves were harvested and snap-frozen under liquid nitrogen. Nuclei were isolated in a high pH Tris buffer to counteract tissue acidity. The nuclei were embedded in low melting point agarose, digested with proteinase K, restriction digested with Hind III and size selected by means of two rounds of pulsed field gel electrophoresis. Large size DNA fragments were ligated into HindIII-cut vector pCC1BACH and transformed by electroporation in to E. coli Transformax EPI300 (Epicentre) cells. 130 clones were assessed for insert size by excision of the insert by NotI digestion and CHEF gel analysis of the products. Most inserts were 80-110 kbp with an average insert size of 100 kbp. 36,864 clones were collected and BAC end sequences were obtained for ~27,000 clones.

text: Primary leaves

text: Vigna unguiculata

text: HindIII

text: VUUBBa (VUH2)

text: Epicenter Copy Control pCC1BAC


text: Washington State University

text: live

text: Generic

text: Lens culinaris subsp. culinaris

text: Lens culinaris subsp. culinaris RFLP probe


text: Stanford Human Genome Center

text: live

text: Generic

text: DH10B (T1 phage resistant)

text: Sorghum bicolor

text: HindIII

text: HindIII

text: Young leaves

characteristic: tissue-type

text: BAC


text: Academia Sinica

text: live

text: Generic

text: adult

text: Sequoia sempervirens

text: shoot

text: DNA fragment from adult Sequoia shoots


text: Federal University of Minas Gerais

text: live

text: Generic

text: Corynebacterium pseudotuberculosis T2 strain genomic library construction was performed with a TOPO Shotgun Subcloning Kit (Invitrogen life technologies, Carlsbad, CA, USA), as recommended by the manufacturers.

text: Corynebacterium pseudotuberculosis

characteristic: strain

text: Corynebacterium pseudotuberculosis T2 genomic library

text: Plasmid

text: pCR 4Blunt Topo

characteristic: vector


characteristic: External Id

text: Purdue University

characteristic: INSDC center name

text: live

text: Generic

text: DH10B-T1 phage resistant

text: Oryza punctata

text: HindIII

text: HindIII

text: young leaves

text: Plasmid


text: The Salk Institute for Biological Studies

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: PCR was performed on Arabidopsis thaliana lines using specific primers. The resultant PCR product was sequenced with either the forward or reverse PCR primer.

text: Arabidopsis thaliana

text: Arabidopsis thaliana strain bay-0 polymorphism sequences


text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Arabidopsis thaliana

text: Arabidopsis thaliana strain van-0 polymorphism sequences


text: live

text: Generic

characteristic: NCBI submission package

text: Arabidopsis thaliana

characteristic: organism

text: Arabidopsis thaliana strain tsu-1 polymorphism sequences


text: live

text: Generic

characteristic: description

text: Arabidopsis thaliana

text: Arabidopsis thaliana strain ts-1 polymorphism sequences

characteristic: title


text: live

text: Generic

text: Arabidopsis thaliana

text: Arabidopsis thaliana strain tamm-2 polymorphism sequences


text: live

text: Generic

text: Arabidopsis thaliana

text: sha

text: Arabidopsis thaliana strain sha polymorphism sequences


text: live

text: Generic

text: Arabidopsis thaliana

text: Arabidopsis thaliana strain rrs-7 polymorphism sequences


text: live

text: Generic

text: Arabidopsis thaliana

text: Arabidopsis thaliana strain rrs-10 polymorphism sequences


text: live

text: Generic

text: Arabidopsis thaliana

text: Arabidopsis thaliana strain nfa-8 polymorphism sequences


text: live

text: Generic

text: Arabidopsis thaliana

text: Arabidopsis thaliana strain lov-5 polymorphism sequences


text: live

text: Generic

text: Arabidopsis thaliana

text: Arabidopsis thaliana strain ler-1 polymorphism sequences


text: live

text: Generic

text: Arabidopsis thaliana

text: Arabidopsis thaliana strain got-7 polymorphism sequences


text: live

text: Generic

text: Arabidopsis thaliana

text: Arabidopsis thaliana strain fei-0 polymorphism sequences


text: live

text: Generic

text: Arabidopsis thaliana

text: Arabidopsis thaliana strain est-1 polymorphism sequences


text: live

text: Generic

text: Arabidopsis thaliana

text: Arabidopsis thaliana strain bor-4 polymorphism sequences


text: The Salk Institute for Biological Studies

text: live

text: Generic

text: Arabidopsis thaliana

text: Arabidopsis thaliana strain br-0 polymorphism sequences


text: live

text: Generic

text: PCR was performed on Arabidopsis thaliana lines using specific primers. The resultant PCR product was sequenced with either the forward or reverse PCR primer.

text: Arabidopsis thaliana

text: Arabidopsis thaliana strain bur-0 polymorphism sequences


text: live

text: Generic

text: Arabidopsis thaliana

text: Arabidopsis thaliana strain c24 polymorphism sequences


text: live

text: Generic

text: Arabidopsis thaliana

text: Arabidopsis thaliana strain cvi-0 polymorphism sequences


text: The Institute for Genomic Research (TIGR; www.tigr.org)

text: live

text: Generic

text: Chinese spring

text: BAC library constructed from short arm of chromosome 3A (3AS) isolated by flow cytometry

text: Triticum aestivum

text: root tips


text: live

text: Generic

text: Solanum tuberosum

text: Solanum tuberosum genomic sequence


text: North Dakota State University

text: live

text: Generic

text: Phaseolus vulgaris

text: Young leaves

text: Phaseolus vulgaris BAT93 young leaves genomic DNA


text: North Dakota State University

text: live

text: Generic

text: Jalo EEP 558

text: Phaseolus vulgaris

text: Young leaves

text: Phaseolus vulgaris Jalo EEP 558 young leaves genomic DNA


text: Kansas State University

text: live

text: Generic

text: Procedure for genomic library construction was previously described in Khramtsov, N.V., M. Tilley, D.S. Blunt, B.A. Montelone, and S.J. Upton. 1995. Cloning and analysis of a Cryptosporidium parvum gene encoding a protein with homology to cytoplasmic form Hsp70. Journal of Eukaryotic Microbiology 42: 416 422.

text: Sporozoites

characteristic: dev-stage

characteristic: isolate

text: Bos tarus

text: Cryptosporidium parvum

text: C. parvum HindIII genomic library


text: Department of Biological sciences of Konkuk University

text: live

text: Generic

text: Artemisia japonica

text: Artemisia japonica genomic DNA

text: T-vector


text: Nanjing Normal University

text: live

text: Generic

text: Dendrobium officinale

text: Genomic sequences from Dendrobium officinale


text: Biozentrum der Universitaet Basel

text: live

text: Generic

text: Drosophila melanogaster

text: line B2020

text: Drosophila melanogaster line B2020


text: Biozentrum der Universitaet Basel

text: live

text: Generic

text: Drosophila melanogaster

text: line B2024

text: Drosophila melanogaster line B2024


text: Biozentrum der Universitaet Basel

text: live

text: Generic

text: Drosophila melanogaster

text: line B0628

text: Drosophila melanogaster line B0628


text: Biozentrum der Universitaet Basel

text: live

text: Generic

text: Drosophila melanogaster

text: line B0826

text: Drosophila melanogaster line B0826


text: University of Minnesota

text: live

text: Generic

text: Felis catus

text: EcoRI

text: RPCI-86 Male Feline BAC

text: BAC


text: The University of Arizona

text: live

text: Generic

text: Genomic DNA was extracted using CTAB (Doyle JJ, 1987) and sheared using the Hydroshear Machine (GeneMachines) to produce 2-5kb fragments.

characteristic: lab-host

text: Oryza alta

text: hgz

text: Plasmid

text: pBluescriptII KS+


text: The University of Arizona

text: live

text: Generic

text: Oryza australiensis

text: hha

text: Plasmid

text: pBluescriptII KS+


text: The University of Arizona

text: live

text: Generic

text: Oryza brachyantha

text: hhb

text: Plasmid

text: pBluescriptII KS+


text: The University of Arizona

text: live

text: Generic

text: Oryza coarctata

text: hhd

text: Plasmid

text: pBluescriptII KS+


text: The University of Arizona

text: live

text: Generic

text: Oryza glaberrima

text: hgu

text: Plasmid

text: pBluescriptII KS+


text: The University of Arizona

text: live

text: Generic

text: Oryza meyeriana var. granulata

text: hhc

text: Plasmid

text: pBluescriptII KS+


text: The University of Arizona

text: live

text: Generic

text: Oryza minuta

text: hgx

text: Plasmid

text: pBluescriptII KS+


text: The University of Arizona

text: live

text: Generic

text: Oryza nivara

text: hhf

text: Plasmid

text: pBluescriptII KS+


text: The University of Arizona

text: live

text: Generic

text: Oryza punctata

text: hgw

text: Plasmid

text: pBluescriptII KS+


text: The University of Arizona

text: live

text: Generic

text: Oryza ridleyi

text: hhe

text: Plasmid

text: pBluescriptII KS+


text: The University of Arizona

text: live

text: Generic

text: Oryza rufipogon

text: hgv

text: Plasmid

text: pBluescriptII KS+


text: The University of Arizona

text: live

text: Generic

text: Oryza officinalis

text: hgy

text: Plasmid

text: pBluescriptII KS+


text: CEA,DSV, iBEB, CNRS, Aix-Marseille Universite

text: live

text: Generic

text: Pseudomonas brassicacearum genes modulated by cadmium

text: Pseudomonas brassicacearum

text: Pseudomonas brassicacearum Cd-Express Library


text: live

text: Generic

text: Embryonic stem cell

characteristic: cell type

text: ES cells 129P2 (formerly 129/Ola)

characteristic: cell-line

text: Mus musculus

text: Male

characteristic: sex

text: 129 Sv

text: GGTC Gene Trap Library GV31C01

text: Retroviral insertion mutagen


text: The University of Arizona

text: live

text: Generic

text: Plainsman

characteristic: cultivar

text: 21 day old

text: DH10B T1 phage resistant

text: Amaranthus hypochondriacus

text: HindIII

text: HindIII

text: leaves

characteristic: tissue-type

characteristic: vector


characteristic: External Id

text: North Dakota State University

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: Phaseolus vulgaris

text: Young leaves

text: Phaseolus vulgaris 5-593 young leaves genomic DNA


text: University of Florence

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Pseudomonas savastanoi pv. savastanoi

characteristic: strain

text: Pseudomonas savastanoi pv. savastanoi ITM317


text: University of Florence

text: live

text: Generic

characteristic: NCBI submission package

text: Pseudomonas savastanoi pv. nerii

characteristic: organism

text: Pseudomonas savastanoi pv. nerii ITM204

characteristic: title


text: University of Florence

text: live

text: Generic

text: Pseudomonas savastanoi pv. fraxini

text: Pseudomonas savastanoi pv. fraxini NCPPB2716


text: National Research Center for Environment and Health

text: live

text: Generic

text: R04 (diploid breeding line)

text: Sugar beet RFLP genomic clones in pBluescript (Sugar beet_RFLP1). Sequence submission managed by GABI Primary Database at RZPD: http://gabi.rzpd.de

characteristic: description

text: Beta vulgaris subsp. vulgaris

text: Sugar Beet RFLP1

text: pBluescript


text: live

text: Generic

text: R04 (diploid breeding line)

text: Sugar beet RFLP genomic clones in pUC18 (Sugar beet_RFLP2). Sequence submission managed by GABI Primary Database at RZPD: http://gabi.rzpd.de

text: Beta vulgaris subsp. vulgaris

text: Sugar Beet RFLP2


text: IRD

text: live

text: Generic

text: IAPAR 59

text: Noir,S., Patheyron S., Combes M.C., Lashermes P., Chalhoub B., 2004 Construction and characterisation of a BAC library for genome analysis of the allotetraploid coffee species (Coffea arabica L.) Theor. Appl. Genet. 109:225-230

text: leaf

characteristic: organ

text: Coffea arabica

text: Hind III

text: BAC coffee library

text: BAC


text: Cornell University College of Veterinary Medicine

text: live

text: Generic

text: Escherichia coli LF82


text: Plant Breeding Institute

text: live

text: Generic

text: High molecular weight DNA was digested methylation-sensitive enzyme PstI and fragments of sizes between 2500 bp and 500 bp cloned. Sequence submission managed by GABI Primary Database http://gabi.rzpd.de

text: Beta vulgaris subsp. vulgaris

text: PstI

text: EcoRI

text: Pro1 addition line


text: University of Modena and Reggio Emilia

text: live

text: Generic

text: hematopoietic cell CD34+

characteristic: cell type

text: Amplified PCR products were shotgun cloned and sequenced

text: Homo sapiens

text: MseI

text: Mavilio hematopoietic cell CD34+


text: Universidade de Mogi das Cruzes

text: live

text: Generic

text: Xylella fastidiosa citrus isolate 36f. The SSH library for each X. Fastidiosa isolate against 9a5c was obtained as described by Agron and coworkers in: Agron PG, Macht M, Radnedge L, Skowronski EW, Miller W, Andersen GL. Use of subtractive hybridization for comprehensive surveys of procaryotic genome differences. FEMS Microbiol. Lett. 2002, 211: 175-182.

characteristic: isolate

text: Competent E. coli cells

characteristic: lab-host

text: Xylella fastidiosa

text: 3' T-overhangs

text: 5' T-overhangs

text: Xylella fastidiosa isolate 36f - SSH against 9a5c

text: Plasmid

text: pGEM-T vector (Promega)


text: Universidade de Mogi das Cruzes

text: live

text: Generic

text: Xylella fastidiosa citrus isolate 56a. The SSH library for each X. Fastidiosa isolate against 9a5c was obtained as described by Agron and coworkers in: Agron PG, Macht M, Radnedge L, Skowronski EW, Miller W, Andersen GL. Use of subtractive hybridization for comprehensive surveys of procaryotic genome differences. FEMS Microbiol. Lett. 2002, 211: 175-182.

text: Competent E. coli cells

text: Xylella fastidiosa

text: 3' T-overhangs

text: 5' T-overhangs

text: Xylella fastidiosa isolate 56a - SSH against 9a5c

text: Plasmid

text: pGEM-T vector (Promega)


text: Universidade de Mogi das Cruzes

text: live

text: Generic

text: Xylella fastidiosa citrus isolate 187b. The SSH library for each X. Fastidiosa isolate against 9a5c was obtained as described by Agron and coworkers in: Agron PG, Macht M, Radnedge L, Skowronski EW, Miller W, Andersen GL. Use of subtractive hybridization for comprehensive surveys of procaryotic genome differences. FEMS Microbiol. Lett. 2002, 211: 175-182.

text: Competent E. coli cells

text: Xylella fastidiosa

text: 3' T-overhangs

text: 5' T-overhangs

text: Xylella fastidiosa isolate 187b - SSH against 9a5c

text: Plasmid

text: pGEM-T vector (Promega)


text: Universidade de Mogi das Cruzes

text: live

text: Generic

text: Xylella fastidiosa citrus isolate 912c. The SSH library for each X. Fastidiosa isolate against 9a5c was obtained as described by Agron and coworkers in: Agron PG, Macht M, Radnedge L, Skowronski EW, Miller W, Andersen GL. Use of subtractive hybridization for comprehensive surveys of procaryotic genome differences. FEMS Microbiol. Lett. 2002, 211: 175-182.

text: Competent E. coli cells

text: Xylella fastidiosa

text: 3' T-overhangs

text: 5' T-overhangs

text: Xylella fastidiosa isolate 912c - SSH against 9a5c

text: Plasmid

text: pGEM-T vector (Promega)


text: Universidade de Mogi das Cruzes

text: live

text: Generic

text: Xylella fastidiosa citrus isolate Cv21. The SSH library for each X. Fastidiosa isolate against 9a5c was obtained as described by Agron and coworkers in: Agron PG, Macht M, Radnedge L, Skowronski EW, Miller W, Andersen GL. Use of subtractive hybridization for comprehensive surveys of procaryotic genome differences. FEMS Microbiol. Lett. 2002, 211: 175-182.

text: Competent E. coli cells

text: Xylella fastidiosa

text: 3' T-overhangs

text: 5' T-overhangs

text: Xylella fastidiosa isolate Cv21 - SSH against 9a5c

text: Plasmid

text: pGEM-T vector (Promega)


text: Universidade de Mogi das Cruzes

text: live

text: Generic

text: Xylella fastidiosa citrus isolate Fb7. The SSH library for each X. Fastidiosa isolate against 9a5c was obtained as described by Agron and coworkers in: Agron PG, Macht M, Radnedge L, Skowronski EW, Miller W, Andersen GL. Use of subtractive hybridization for comprehensive surveys of procaryotic genome differences. FEMS Microbiol. Lett. 2002, 211: 175-182.

text: Competent E. coli cells

text: Xylella fastidiosa

text: 3' T-overhangs

text: 5' T-overhangs

text: Xylella fastidiosa isolate Fb7 - SSH against 9a5c

text: Plasmid

text: pGEM-T vector (Promega)


text: University of Dundee at SCRI

text: live

text: Generic

text: Vicia faba

text: Vicia faba DNA (Flavell AJ)


text: University of Dundee at SCRI

text: live

text: Generic

text: Vicia melanops

text: Vicia melanops DNA (Flavell AJ)


text: University of Dundee at SCRI

text: live

text: Generic

text: Vicia sativa

text: Vicia sativa DNA (Flavell AJ)


text: Texas A&M Institute for Genomic Medicine (TIGM)

text: live

text: Generic

text: embryonic stem cell

text: Lex3.13 ES transgenic cell line harboring insertion mutation

characteristic: cell-line

text: strain origin confirmed on the basis of 110 genetic markers distributed across 19 autosomes and the X chromosome (Charles River Laboratories); sequence tags are derived from genomic sequence by inverse PCR (IPCR) and represent the gene-trap vector insertion site; sequence reads represent either the upstream (F) or downstream (R) vector-genomic junction, amplified from circularized genomic DNA.

text: Mus musculus

text: male

characteristic: sex

text: TIGM C57BL/6N Gene Trap Library


characteristic: External Id

text: China Agricultural University

text: live

text: Generic

text: Gallus gallus

text: Chicken BAC library


text: Imperial College London

characteristic: INSDC center name

text: live

text: Generic

text: embryonic fibroblasts

text: Genomic DNA from HIV-1-infected PSIP1(f/+) MEFs was digested with a mixture of AvrII, NheI, and SpeI and ligated with 5'-GTAATACGACTCACTATAGGGCACGCGTGGTCGACGGCCCGGGCTGC/ 5'-PO4-CTAGGCAGCCCG-AmC7Q linker. Sequences PCR-amplified using primers 5'-GACTCACTATAGGGCACGCGT/5'-GAGGGATCTCTAGTTACCAGAGTCACA were nested using 5'-GTCGACGGCCCGGGCTGCCTA/5'-AGCCAGAGAGCTCCCAGGCTCAGATC. Resulting PCR products subcloned into pCR4.1-TOPO were sequenced using 5'-CCAAAGGTCAGTGGATATCTG. Sequences were parsed to remove U3 and linker-derived portions;low quality sequences and sequences that did not contain the processed U3 terminus (5'-TTAGCCCTTCCA-3'), or with less than 16 bp of genomic DNA between the processed U3 end and beginning of the linker sequence, were discarded.

text: Mus musculus

text: AvrII+SpeI+NheI

text: plasmid

characteristic: vector


text: Imperial College London

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: embryonic fibroblasts

text: Genomic DNA from HIV-1-infected PSIP1(-/-) MEFs was digested with a mixture of AvrII, NheI, and SpeI and ligated with 5'-GTAATACGACTCACTATAGGGCACGCGTGGTCGACGGCCCGGGCTGC/ 5'-PO4-CTAGGCAGCCCG-AmC7Q linker. Sequences PCR-amplified using primers 5'-GACTCACTATAGGGCACGCGT/5'-GAGGGATCTCTAGTTACCAGAGTCACA were nested using 5'-GTCGACGGCCCGGGCTGCCTA/5'-AGCCAGAGAGCTCCCAGGCTCAGATC. Resulting PCR products subcloned into pCR4.1-TOPO were sequenced using 5'-CCAAAGGTCAGTGGATATCTG. Sequences were parsed to remove U3 and linker-derived portions;low quality sequences and sequences that did not contain the processed U3 terminus (5'-TTAGCCCTTCCA-3'), or with less than 16 bp of genomic DNA between the processed U3 end and beginning of the linker sequence, were discarded.

text: Mus musculus

text: AvrII+SpeI+NheI

text: plasmid


text: Korea Forest Research Institute

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: High-molecular-weight DNA was extracted from leaves of Populus nigra var. italica. Partial genomic region corresponding to P. nigra 5'-end EST sequence of Zinc finger protein (Accession no. BP925347) was amplified and directly sequenced at both ends.

text: Populus nigra

text: Populus nigra PCR fragment for ZF 5'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

characteristic: NCBI submission package

text: High-molecular-weight DNA was extracted from leaves of Populus tremula var. davidiana selection clone Bukdae-12. Partial genomic region corresponding to P. nigra 5'-end EST sequence of Zinc finger protein (Accession no. BP925347) was amplified and directly sequenced at both ends.

text: selection clone Bukdae-12

text: Populus davidiana

text: Populus tremula var. davidiana Bukdae-12 PCR fragment for ZF 5'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus tremula var. davidiana selection clone Sogwang-4. Partial genomic region corresponding to P. nigra 5'-end EST sequence of Zinc finger protein (Accession no. BP925347) was amplified and directly sequenced at both ends.

text: selection clone Sogwang-4

text: Populus davidiana

characteristic: organism

text: Populus tremula var. davidiana Sogwang-4 PCR fragment for ZF 5'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus tremula var. davidiana selection clone Ssangjeon-3. Partial genomic region corresponding to P. nigra 5'-end EST sequence of Zinc finger protein (Accession no. BP925347) was amplified and directly sequenced at both ends.

text: selection clone Ssangjeon-3

text: Populus davidiana

text: Populus tremula var. davidiana Ssangjeon-3 PCR fragment for ZF 5'-end EST

characteristic: title


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus tremula var. davidiana selection clone Ssangjeon-9. Partial genomic region corresponding to P. nigra 5'-end EST sequence of Zinc finger protein (Accession no. BP925347) was amplified and directly sequenced at both ends.

characteristic: description

text: selection clone Ssangjeon-9

text: Populus davidiana

text: Populus tremula var. davidiana Ssangjeon-9 PCR fragment for ZF 5'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus tremula var. davidiana selection clone Odae-19. Partial genomic region corresponding to P. nigra 5'-end EST sequence of Zinc finger protein (Accession no. BP925347) was amplified and directly sequenced at both ends.

text: selection clone Odae-19

text: Populus davidiana

text: Populus tremula var. davidiana Odae-19 PCR fragment for ZF 5'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus tremula var. davidiana selection clone Undu-2. Partial genomic region corresponding to P. nigra 5'-end EST sequence of Zinc finger protein (Accession no. BP925347) was amplified and directly sequenced at both ends.

text: selection clone Undu-2

text: Populus davidiana

text: Populus tremula var. davidiana Undu-2 PCR fragment for ZF 5'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus tremula var. davidiana selection clone Bangdong-1. Partial genomic region corresponding to P. nigra 5'-end EST sequence of Zinc finger protein (Accession no. BP925347) was amplified and directly sequenced at both ends.

text: selection clone Bangdong-1

text: Populus davidiana

text: Populus tremula var. davidiana Bangdong-1 PCR fragment for ZF 5'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus tremula var. davidiana selection clone Bukdae-12. Partial genomic region corresponding to P. nigra 3'-end EST sequence of Amino acid permease I(Accession no. BP930782) was amplified and directly sequenced at both ends.

text: selection clone Bukdae-12

text: Populus davidiana

text: Populus tremula var. davidiana Bukdae-12 PCR fragment for AAP1 3'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus tremula var. davidiana selection clone Sogwang-4. Partial genomic region corresponding to P. nigra 3'-end EST sequence of Amino acid permease I(Accession no. BP930782) was amplified and directly sequenced at both ends.

text: selection clone Sogwang-4

text: Populus davidiana

text: Populus tremula var. davidiana Sogwang-4 PCR fragment for AAP1 3'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus tremula var. davidiana selection clone Ssangjeon-3. Partial genomic region corresponding to P. nigra 3'-end EST sequence of Amino acid permease I(Accession no. BP930782) was amplified and directly sequenced at both ends.

text: selection clone Ssangjeon-3

text: Populus davidiana

text: Populus tremula var. davidiana Ssangjeon-3 PCR fragment for AAP1 3'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus tremula var. davidiana selection clone Ssangjeon-9. Partial genomic region corresponding to P. nigra 3'-end EST sequence of Amino acid permease I(Accession no. BP930782) was amplified and directly sequenced at both ends.

text: selection clone Ssangjeon-9

text: Populus davidiana

text: Populus tremula var. davidiana Ssangjeon-9 PCR fragment for AAP1 3'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus tremula var. davidiana selection clone Odae-19. Partial genomic region corresponding to P. nigra 3'-end EST sequence of Amino acid permease I(Accession no. BP930782) was amplified and directly sequenced at both ends.

text: selection clone Odae-19

text: Populus davidiana

text: Populus tremula var. davidiana Odae-19 PCR fragment for AAP1 3'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus tremula var. davidiana selection clone Undu-2. Partial genomic region corresponding to P. nigra 3'-end EST sequence of Amino acid permease I(Accession no. BP930782) was amplified and directly sequenced at both ends.

text: selection clone Undu-2

text: Populus davidiana

text: Populus tremula var. davidiana Undu-2 PCR fragment for AAP1 3'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus tremula var. davidiana selection clone Bangdong-1. Partial genomic region corresponding to P. nigra 3'-end EST sequence of Amino acid permease I(Accession no. BP930782) was amplified and directly sequenced at both ends.

text: selection clone Bangdong-1

text: Populus davidiana

text: Populus tremula var. davidiana Bangdong-1 PCR fragment for AAP1 3'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus nigra var. italica. Partial genomic region corresponding to P. nigra 3'-end EST sequence of Amino acid permease I(Accession no. BP930782) was amplified and directly sequenced at both ends.

text: Populus nigra

text: Populus nigra PCR fragment for AAP1 3'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus tremula var. davidiana selection clone Ssangjeon-9. Partial genomic region corresponding to P. nigra 5'-end EST sequence of Amino acid permease I(Accession no. BP923026) was amplified and directly sequenced at both ends.

text: selection clone Ssangjeon-9

text: Populus davidiana

text: Populus tremula var. davidiana Ssangjeon-9 PCR fragment for AAP1 5'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus tremula var. davidiana selection clone Odae-19. Partial genomic region corresponding to P. nigra 5'-end EST sequence of Amino acid permease I(Accession no. BP923026) was amplified and directly sequenced at both ends.

text: selection clone Odae-19

text: Populus davidiana

text: Populus tremula var. davidiana Odae-19 PCR fragment for AAP1 5'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus tremula var. davidiana selection clone Undu-2. Partial genomic region corresponding to P. nigra 5'-end EST sequence of Amino acid permease I(Accession no. BP923026) was amplified and directly sequenced at both ends.

text: selection clone Undu-2

text: Populus davidiana

text: Populus tremula var. davidiana Undu-2 PCR fragment for AAP1 5'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus nigra var. italica. Partial genomic region corresponding to P. nigra 5'-end EST sequence of Amino acid permease I(Accession no. BP923026) was amplified and directly sequenced at both ends.

text: Populus nigra

text: Populus nigra PCR fragment for AAP1 5'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Salix pseudolasiogyne. Partial genomic region corresponding to P. nigra 5'-end EST sequence of Alternative oxidase 1a(Accession no. BP922653) was amplified and directly sequenced at both ends.

text: Salix pseudolasiogyne

text: Salix pseudolasiogyne PCR fragment for AOX 5'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus alba x Populus tremula var. glandulosa. Partial genomic region corresponding to P. nigra 5'-end EST sequence of Alternative oxidase 1a(Accession no. BP922653) was amplified and directly sequenced at both ends.

text: Populus alba x Populus glandulosa

text: Populus alba x Populus tremula var. glandulosa PCR fragment for AOX 5'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus tremula var. davidiana selection clone Undu-2. Partial genomic region corresponding to P. nigra 5'-end EST sequence of Alternative oxidase 1a(Accession no. BP922653) was amplified and directly sequenced at both ends.

text: selection clone Undu-2

text: Populus davidiana

text: Populus tremula var. davidiana Undu-2 PCR fragment for AOX 5'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus tremula var. davidiana selection clone Jungguk-6. Partial genomic region corresponding to P. nigra 5'-end EST sequence of Alternative oxidase 1a(Accession no. BP922653) was amplified and directly sequenced at both ends.

text: selection clone Jungguk-6

text: Populus davidiana

text: Populus tremula var. davidiana Jungguk-6 PCR fragment for AOX 5'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus tremula var. davidiana selection clone Palgong-2. Partial genomic region corresponding to P. nigra 5'-end EST sequence of Alternative oxidase 1a(Accession no. BP922653) was amplified and directly sequenced at both ends.

text: selection clone Palgong-2

text: Populus davidiana

text: Populus tremula var. davidiana Palgong-2 PCR fragment for AOX 5'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus x canadensis selection clone italy-1. Partial genomic region corresponding to P. nigra 5'-end EST sequence of Alternative oxidase 1a(Accession no. BP922653) was amplified and directly sequenced at both ends.

text: selection clone italy-1

text: Populus x canadensis

text: Populus x canadensis italy-1 PCR fragment for AOX 5'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus x canadensis selection clone italy-2. Partial genomic region corresponding to P. nigra 5'-end EST sequence of Alternative oxidase 1a(Accession no. BP922653) was amplified and directly sequenced at both ends.

text: selection clone italy-2

text: Populus x canadensis

text: Populus x canadensis italy-2 PCR fragment for AOX 5'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus tremula var. davidiana selection clone Taebaek-1. Partial genomic region corresponding to P. nigra 5'-end EST sequence of Alternative oxidase 1a(Accession no. BP922653) was amplified and directly sequenced at both ends.

text: selection clone Taebaek-1

text: Populus davidiana

text: Populus tremula var. davidiana Taebaek-1 PCR fragment for AOX 5'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus nigra var. italica. Partial genomic region corresponding to P. nigra 5'-end EST sequence of Alternative oxidase 1a(Accession no. BP922653) was amplified and directly sequenced at both ends.

text: Populus nigra

text: Populus nigra PCR fragment for AOX 5'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus tremula var. davidiana selection clone Bukdae-12. Partial genomic region corresponding to P. nigra 5'-end EST sequence of Ethylene-responsive element-binding factor 1(Accession no. BP924591) was amplified and directly sequenced at both ends.

text: selection clone Bukdae-12

text: Populus davidiana

text: Populus tremula var. davidiana Bukdae-12 PCR fragment for ERF1 5'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus tremula var. davidiana selection clone Sogwang-4. Partial genomic region corresponding to P. nigra 5'-end EST sequence of Ethylene-responsive element-binding factor 1(Accession no. BP924591) was amplified and directly sequenced at both ends.

text: selection clone Sogwang-4

text: Populus davidiana

text: Populus tremula var. davidiana Sogwang-4 PCR fragment for ERF1 5'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus tremula var. davidiana selection clone Ssangjeon-3. Partial genomic region corresponding to P. nigra 5'-end EST sequence of Ethylene-responsive element-binding factor 1(Accession no. BP924591) was amplified and directly sequenced at both ends.

text: selection clone Ssangjeon-3

text: Populus davidiana

text: Populus tremula var. davidiana Ssangjeon-3 PCR fragment for ERF1 5'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus tremula var. davidiana selection clone Ssangjeon-9. Partial genomic region corresponding to P. nigra 5'-end EST sequence of Ethylene-responsive element-binding factor 1(Accession no. BP924591) was amplified and directly sequenced at both ends.

text: selection clone Ssangjeon-9

text: Populus davidiana

text: Populus tremula var. davidiana Ssangjeon-9 PCR fragment for ERF1 5'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus tremula var. davidiana selection clone Odae-19. Partial genomic region corresponding to P. nigra 5'-end EST sequence of Ethylene-responsive element-binding factor 1(Accession no. BP924591) was amplified and directly sequenced at both ends.

text: selection clone Odae-19

text: Populus davidiana

text: Populus tremula var. davidiana Odae-19 PCR fragment for ERF1 5'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus tremula var. davidiana selection clone Undu-2. Partial genomic region corresponding to P. nigra 5'-end EST sequence of Ethylene-responsive element-binding factor 1(Accession no. BP924591) was amplified and directly sequenced at both ends.

text: selection clone Undu-2

text: Populus davidiana

text: Populus tremula var. davidiana Undu-2 PCR fragment for ERF1 5'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus tremula var. davidiana selection clone Bangdong-1. Partial genomic region corresponding to P. nigra 5'-end EST sequence of Ethylene-responsive element-binding factor 1(Accession no. BP924591) was amplified and directly sequenced at both ends.

text: selection clone Bangdong-1

text: Populus davidiana

text: Populus tremula var. davidiana Bangdong-1 PCR fragment for ERF1 5'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus nigra var. italica. Partial genomic region corresponding to P. nigra 5'-end EST sequence of Ethylene-responsive element-binding factor 1(Accession no. BP924591) was amplified and directly sequenced at both ends.

text: Populus nigra

text: Populus nigra PCR fragment for ERF1 5'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Salix pseudolasiogyne. Partial genomic region corresponding to P. nigra 5'-end EST sequence of Galactinol synthase(Accession no. BP922497) was amplified and directly sequenced at both ends.

text: Salix pseudolasiogyne

text: Salix pseudolasiogyne PCR fragment for GS 5'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus alba x Populus tremula var. glandulosa. Partial genomic region corresponding to P. nigra 5'-end EST sequence of Galactinol synthase(Accession no. BP922497) was amplified and directly sequenced at both ends.

text: Populus alba x Populus glandulosa

text: Populus alba x Populus tremula var. glandulosa PCR fragment for GS 5'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus tremula var. davidiana selection clone Undu-2. Partial genomic region corresponding to P. nigra 5'-end EST sequence of Galactinol synthase(Accession no. BP922497) was amplified and directly sequenced at both ends.

text: selection clone Undu-2

text: Populus davidiana

text: Populus tremula var. davidiana Undu-2 PCR fragment for GS 5'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus tremula var. davidiana selection clone Jungkuk-6. Partial genomic region corresponding to P. nigra 5'-end EST sequence of Galactinol synthase(Accession no. BP922497) was amplified and directly sequenced at both ends.

text: selection clone Jungkuk-6

text: Populus davidiana

text: Populus tremula var. davidiana Jungkuk-6 PCR fragment for GS 5'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus tremula var. davidiana selection clone Taebaek-1. Partial genomic region corresponding to P. nigra 5'-end EST sequence of Galactinol synthase(Accession no. BP922497) was amplified and directly sequenced at both ends.

text: selection clone Taebaek-1

text: Populus davidiana

text: Populus tremula var. davidiana Taebaek-1 PCR fragment for GS 5'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus tremula var. davidiana selection clone Palgong-2. Partial genomic region corresponding to P. nigra 5'-end EST sequence of Galactinol synthase(Accession no. BP922497) was amplified and directly sequenced at both ends.

text: selection clone Palgong-2

text: Populus davidiana

text: Populus tremula var. davidiana Palgong-2 PCR fragment for GS 5'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Salix pseudolasiogyne. Partial genomic region corresponding to P. nigra 3'-end EST sequence of 4-Hydroxyphenylpyruvate dioxygenase(Accession no. BP930649) was amplified and directly sequenced at both ends.

text: Salix pseudolasiogyne

text: Salix pseudolasiogyne PCR fragment for HPD 3'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus nigra var. italica. Partial genomic region corresponding to P. nigra 3'-end EST sequence of 4-Hydroxyphenylpyruvate dioxygenase(Accession no. BP930649) was amplified and directly sequenced at both ends.

text: Populus nigra

text: Populus nigra PCR fragment for HPD 3'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus tremula var. davidiana selection clone Sogwang-4. Partial genomic region corresponding to P. nigra 3'-end EST sequence of 4-Hydroxyphenylpyruvate dioxygenase(Accession no. BP930649) was amplified and directly sequenced at both ends.

text: selection clone Sogwang-4

text: Populus davidiana

text: Populus tremula var. davidiana Sogwang-4 PCR fragment for HPD 3'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus tremula var. davidiana selection clone Ssangjeon-3. Partial genomic region corresponding to P. nigra 3'-end EST sequence of 4-Hydroxyphenylpyruvate dioxygenase(Accession no. BP930649) was amplified and directly sequenced at both ends.

text: selection clone Ssangjeon-3

text: Populus davidiana

text: Populus tremula var. davidiana Ssangjeon-3 PCR fragment for HPD 3'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus tremula var. davidiana selection clone Ssangjeon-9. Partial genomic region corresponding to P. nigra 3'-end EST sequence of 4-Hydroxyphenylpyruvate dioxygenase(Accession no. BP930649) was amplified and directly sequenced at both ends.

text: selection clone Ssangjeon-9

text: Populus davidiana

text: Populus tremula var. davidiana Ssangjeon-9 PCR fragment for HPD 3'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus tremula var. davidiana selection clone Odae-19. Partial genomic region corresponding to P. nigra 3'-end EST sequence of 4-Hydroxyphenylpyruvate dioxygenase(Accession no. BP930649) was amplified and directly sequenced at both ends.

text: selection clone Odae-19

text: Populus davidiana

text: Populus tremula var. davidiana Odae-19 PCR fragment for HPD 3'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus tremula var. davidiana selection clone Undu-2. Partial genomic region corresponding to P. nigra 3'-end EST sequence of 4-Hydroxyphenylpyruvate dioxygenase(Accession no. BP930649) was amplified and directly sequenced at both ends.

text: selection clone Undu-2

text: Populus davidiana

text: Populus tremula var. davidiana Undu-2 PCR fragment for HPD 3'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus tremula var. davidiana selection clone Bangdong-1. Partial genomic region corresponding to P. nigra 3'-end EST sequence of 4-Hydroxyphenylpyruvate dioxygenase(Accession no. BP930649) was amplified and directly sequenced at both ends.

text: selection clone Bangdong-1

text: Populus davidiana

text: Populus tremula var. davidiana Bangdong-1 PCR fragment for HPD 3'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Salix pseudolasiogyne. Partial genomic region corresponding to P. nigra 5'-end EST sequence of 4-Hydroxyphenylpyruvate dioxygenase(Accession no. BP922875) was amplified and directly sequenced at both ends.

text: Salix pseudolasiogyne

text: Salix pseudolasiogyne PCR fragment for HPD 5'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus alba x Populus tremula var. glandulosa. Partial genomic region corresponding to P. nigra 5'-end EST sequence of 4-Hydroxyphenylpyruvate dioxygenase(Accession no. BP922875) was amplified and directly sequenced at both ends.

text: Populus alba x Populus glandulosa

text: Populus alba x Populus tremula var. glandulosa PCR fragment for HPD 5'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus tremula var. davidiana selection clone Undu-2. Partial genomic region corresponding to P. nigra 5'-end EST sequence of 4-Hydroxyphenylpyruvate dioxygenase(Accession no. BP922875) was amplified and directly sequenced at both ends.

text: selection clone Undu-2

text: Populus davidiana

text: Populus tremula var. davidiana Undu-2 PCR fragment for HPD 5'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus tremula var. davidiana selection clone Jungkuk-6. Partial genomic region corresponding to P. nigra 5'-end EST sequence of 4-Hydroxyphenylpyruvate dioxygenase(Accession no. BP922875) was amplified and directly sequenced at both ends.

text: selection clone Jungkuk-6

text: Populus davidiana

text: Populus tremula var. davidiana Jungkuk-6 PCR fragment for HPD 5'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus tremula var. davidiana selection clone Ssangjeon-9. Partial genomic region corresponding to P. nigra 5'-end EST sequence of 4-Hydroxyphenylpyruvate dioxygenase(Accession no. BP922875) was amplified and directly sequenced at both ends.

text: selection clone Ssangjeon-9

text: Populus davidiana

text: Populus tremula var. davidiana Ssangjeon-9 PCR fragment for HPD 5'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus tremula var. davidiana selection clone Odae-19. Partial genomic region corresponding to P. nigra 5'-end EST sequence of 4-Hydroxyphenylpyruvate dioxygenase(Accession no. BP922875) was amplified and directly sequenced at both ends.

text: selection clone Odae-19

text: Populus davidiana

text: Populus tremula var. davidiana Odae-19 PCR fragment for HPD 5'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus tremula var. davidiana selection clone Bangdong-1. Partial genomic region corresponding to P. nigra 5'-end EST sequence of 4-Hydroxyphenylpyruvate dioxygenase(Accession no. BP922875) was amplified and directly sequenced at both ends.

text: selection clone Bangdong-1

text: Populus davidiana

text: Populus tremula var. davidiana Bangdong-1 PCR fragment for HPD 5'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus nigra var. italica. Partial genomic region corresponding to P. nigra 5'-end EST sequence of 4-Hydroxyphenylpyruvate dioxygenase(Accession no. BP922875) was amplified and directly sequenced at both ends.

text: Populus nigra

text: Populus nigra PCR fragment for HPD 5'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus nigra var. italica. Partial genomic region corresponding to P. nigra 3'-end EST sequence of Leucoanthocyanidin dioxygenase-like protein (Accession no. BP934164) was amplified and directly sequenced at both ends.

text: Populus nigra

text: Populus nigra PCR fragment for LAD 3'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus tremula var. davidiana selection clone Sogwang-4. Partial genomic region corresponding to P. nigra 3'-end EST sequence of Leucoanthocyanidin dioxygenase-like protein (Accession no. BP934164) was amplified and directly sequenced at both ends.

text: selection clone Sogwang-4

text: Populus davidiana

text: Populus tremula var. davidiana Sogwang-4 PCR fragment for LAD 3'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus tremula var. davidiana selection clone Ssangjeon-3. Partial genomic region corresponding to P. nigra 3'-end EST sequence of Leucoanthocyanidin dioxygenase-like protein (Accession no. BP934164) was amplified and directly sequenced at both ends.

text: selection clone Ssangjeon-3

text: Populus davidiana

text: Populus tremula var. davidiana Ssangjeon-3 PCR fragment for LAD 3'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus tremula var. davidiana selection clone Ssangjeon-9. Partial genomic region corresponding to P. nigra 3'-end EST sequence of Leucoanthocyanidin dioxygenase-like protein (Accession no. BP934164) was amplified and directly sequenced at both ends.

text: selection clone Ssangjeon-9

text: Populus davidiana

text: Populus tremula var. davidiana Ssangjeon-9 PCR fragment for LAD 3'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus tremula var. davidiana selection clone Odae-19. Partial genomic region corresponding to P. nigra 3'-end EST sequence of Leucoanthocyanidin dioxygenase-like protein (Accession no. BP934164) was amplified and directly sequenced at both ends.

text: selection clone Odae-19

text: Populus davidiana

text: Populus tremula var. davidiana Odae-19 PCR fragment for LAD 3'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus tremula var. davidiana selection clone Undu-2. Partial genomic region corresponding to P. nigra 3'-end EST sequence of Leucoanthocyanidin dioxygenase-like protein (Accession no. BP934164) was amplified and directly sequenced at both ends.

text: selection clone Undu-2

text: Populus davidiana

text: Populus tremula var. davidiana Undu-2 PCR fragment for LAD 3'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus nigra var. italica. Partial genomic region corresponding to P. nigra 5'-end EST sequence of Leucoanthocyanidin dioxygenase-like protein (Accession no. BP926606) was amplified and directly sequenced at both ends.

text: Populus nigra

text: Populus nigra PCR fragment for LAD 5'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus tremula var. davidiana selection clone Bukdae-12. Partial genomic region corresponding to P. nigra 5'-end EST sequence of Leucoanthocyanidin dioxygenase-like protein (Accession no. BP926606) was amplified and directly sequenced at both ends.

text: selection clone Bukdae-12

text: Populus davidiana

text: Populus tremula var. davidiana Bukdae-12 PCR fragment for LAD 5'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus tremula var. davidiana selection clone Sogwang-4. Partial genomic region corresponding to P. nigra 5'-end EST sequence of Leucoanthocyanidin dioxygenase-like protein (Accession no. BP926606) was amplified and directly sequenced at both ends.

text: selection clone Sogwang-4

text: Populus davidiana

text: Populus tremula var. davidiana Sogwang-4 PCR fragment for LAD 5'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus tremula var. davidiana selection clone Ssangjeon-3. Partial genomic region corresponding to P. nigra 5'-end EST sequence of Leucoanthocyanidin dioxygenase-like protein (Accession no. BP926606) was amplified and directly sequenced at both ends.

text: selection clone Ssangjeon-3

text: Populus davidiana

text: Populus tremula var. davidiana Ssangjeon-3 PCR fragment for LAD 5'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus tremula var. davidiana selection clone Ssangjeon-9. Partial genomic region corresponding to P. nigra 5'-end EST sequence of Leucoanthocyanidin dioxygenase-like protein (Accession no. BP926606) was amplified and directly sequenced at both ends.

text: selection clone Ssangjeon-9

text: Populus davidiana

text: Populus tremula var. davidiana Ssangjeon-9 PCR fragment for LAD 5'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus tremula var. davidiana selection clone Odae-19. Partial genomic region corresponding to P. nigra 5'-end EST sequence of Leucoanthocyanidin dioxygenase-like protein (Accession no. BP926606) was amplified and directly sequenced at both ends.

text: selection clone Odae-19

text: Populus davidiana

text: Populus tremula var. davidiana Odae-19 PCR fragment for LAD 5'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus tremula var. davidiana selection clone Undu-2. Partial genomic region corresponding to P. nigra 5'-end EST sequence of Leucoanthocyanidin dioxygenase-like protein (Accession no. BP926606) was amplified and directly sequenced at both ends.

text: selection clone Undu-2

text: Populus davidiana

text: Populus tremula var. davidiana Undu-2 PCR fragment for LAD 5'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus tremula var. davidiana selection clone Bangdong-1. Partial genomic region corresponding to P. nigra 5'-end EST sequence of Leucoanthocyanidin dioxygenase-like protein (Accession no. BP926606) was amplified and directly sequenced at both ends.

text: selection clone Bangdong-1

text: Populus davidiana

text: Populus tremula var. davidiana Bangdong-1 PCR fragment for LAD 5'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus alba x Populus tremula var. glandulosa. Partial genomic region corresponding to P. nigra 3'-end EST sequence of transcription factor like protein (Accession no. BP930753) was amplified and directly sequenced at both ends.

text: Populus alba x Populus glandulosa

text: Populus alba x Populus tremula var. glandulosa PCR fragment for TF 3'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus tremula var. davidiana selection clone Bukdae-12. Partial genomic region corresponding to P. nigra 3'-end EST sequence of transcription factor like protein (Accession no. BP930753) was amplified and directly sequenced at both ends.

text: selection clone Bukdae-12

text: Populus davidiana

text: Populus tremula var. davidiana Bukdae-12 PCR fragment for TF 3'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus tremula var. davidiana selection clone Ssangjeon-3. Partial genomic region corresponding to P. nigra 3'-end EST sequence of transcription factor like protein (Accession no. BP930753) was amplified and directly sequenced at both ends.

text: selection clone Ssangjeon-3

text: Populus davidiana

text: Populus tremula var. davidiana Ssangjeon-3 PCR fragment for TF 3'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus tremula var. davidiana selection clone Ssangjeon-9. Partial genomic region corresponding to P. nigra 3'-end EST sequence of transcription factor like protein (Accession no. BP930753) was amplified and directly sequenced at both ends.

text: selection clone Ssangjeon-9

text: Populus davidiana

text: Populus tremula var. davidiana Ssangjeon-9 PCR fragment for TF 3'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus tremula var. davidiana selection clone Bangdong-1. Partial genomic region corresponding to P. nigra 3'-end EST sequence of transcription factor like protein (Accession no. BP930753) was amplified and directly sequenced at both ends.

text: selection clone Bangdong-1

text: Populus davidiana

text: Populus tremula var. davidiana Bangdong-1 PCR fragment for TF 3'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus nigra var. italica. Partial genomic region corresponding to P. nigra 3'-end EST sequence of Zinc finger protein (Accession no. BP932979) was amplified and directly sequenced at both ends.

text: Populus nigra

text: Populus nigra PCR fragment for ZF 3'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus tremula var. davidiana selection clone Bukdae-12. Partial genomic region corresponding to P. nigra 3'-end EST sequence of Zinc finger protein (Accession no. BP932979) was amplified and directly sequenced at both ends.

text: selection clone Bukdae-12

text: Populus davidiana

text: Populus tremula var. davidiana Bukdae-12 PCR fragment for ZF 3'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus tremula var. davidiana selection clone Sogwang-4. Partial genomic region corresponding to P. nigra 3'-end EST sequence of Zinc finger protein (Accession no. BP932979) was amplified and directly sequenced at both ends.

text: selection clone Sogwang-4

text: Populus davidiana

text: Populus tremula var. davidiana Sogwang-4 PCR fragment for ZF 3'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus tremula var. davidiana selection clone Ssangjeon-3. Partial genomic region corresponding to P. nigra 3'-end EST sequence of Zinc finger protein (Accession no. BP932979) was amplified and directly sequenced at both ends.

text: selection clone Ssangjeon-3

text: Populus davidiana

text: Populus tremula var. davidiana Ssangjeon-3 PCR fragment for ZF 3'-end EST


text: Korea Forest Research Institute

text: live

text: Generic

text: High-molecular-weight DNA was extracted from leaves of Populus tremula var. davidiana selection clone Bangdong-1. Partial genomic region corresponding to P. nigra 3'-end EST sequence of Zinc finger protein (Accession no. BP932979) was amplified and directly sequenced at both ends.

text: selection clone Bangdong-1

text: Populus davidiana

text: Populus tremula var. davidiana Bangdong-1 PCR fragment for ZF 3'-end EST


text: Southern Illinois University at Carbondale and The Institute for Genomic Research

text: live

text: Generic

text: Forrest

characteristic: cultivar

text: Soybean (Glycine max (L.) Merr.) cv. Forrest seeds were grown in greenhouse for fourteen days. Nuclei size in MTP4E was 175 kbp. MTP4E can be viewed at http://soybeangenome.siu.edu. The MTP4E clones were end sequenced.

text: Glycine max

text: EcoRI

text: leaves

characteristic: tissue-type

text: pECBAC1 (EcoRI clones)


text: Carnegie Institution of Washington

text: live

text: Generic

text: Geranium dissectum genomic DNA was probed with appropriate primers for the genes of interest and the products sequenced.

text: Jasper Ridge Biological Preserve, Stanford, CA

characteristic: isolate

text: Leaf

characteristic: organ

text: Geranium dissectum

text: Leaf

text: Geranium dissectum Jasper Ridge Biological Preserve leaves


text: Murdoch University

text: live

text: Generic

text: Phytophthora cinnamomi

text: Phytophthora cinnamomi RAPD-PCR amplicon


text: Yamaguchi University

text: live

text: Generic

text: Hagi

text: Plasmodiophora brassicae

text: Plasmodiophora brassicae isolate Hagi


text: University of California, Davis, and The Institute for Genomic Research

text: live

text: Generic

text: Chickpea (Cicer arietinum) accession ICC4958 was grown under greenhouse conditions for 6 weeks and transferred to continuous darkness for 2 days prior to use. Nuclei were isolated and embedded in low melting point agarose, restriction digested with Hind III and size selected by means of two rounds of pulsed field gel electrophoresis. Large size DNA fragments were ligated in vector pCCBAC1H and transformed by electroporation in to Epicenter's E. coli EPI300-T1R cells. 55,680 clones from were obtained, with most inserts ranging from 100 to 130 kbp. BAC end sequences were obtained from ~25,000 clones.

text: Leaves

text: Cicer arietinum

text: HindIII

text: Epicenter Copy Control pCCBAC1H (HindIII partial digest clones)


text: Iowa State University

text: live

text: Generic

text: avian pathogenic strain

text: Escherichia coli

text: APEC 113

characteristic: strain

text: Escherichia coli APEC 113


text: Iowa State University

text: live

text: Generic

text: Human uropathogenic strain

text: Escherichia coli

text: UPEC 88

text: Escherichia coli UPEC 88


text: Indian Agricultural Research Institute

text: live

text: Generic

text: RAPD fragment of Fusarium oxysporum f. sp. cucumerinum a fungus causing wilt disease of Cucumber in India. RAPD-PCR fragment has been excised from the gel, cloned into PGem-Teasy vector and sequenced to designed a specific primer

text: vegetative (asexual stage)

characteristic: dev-stage

text: Fusarium oxysporum f. sp. cucumerinum

text: CRT-1 (pathogenic group II)

text: mycelium

text: RAPD fragment of Fusarium oxysporum f. sp. cucumerinum CRT-1


characteristic: External Id

text: Indian Agricultural Research Institute

text: live

text: Generic

text: RAPD fragment of Fusarium oxysporum f. sp. cucumerinum a fungus causing wilt disease of Cucumber in India. RAPD -PCR fragment has been excised from the gel, cloned into PGem-Teasy vector and sequenced to designed a specific primer

text: vegetative (asexual stage)

text: Fusarium oxysporum f. sp. cucumerinum

text: asexual (sexual form is unknown)

characteristic: sex

text: CRB-1 (pathogenic group IV)

text: mycelium

text: RAPD fragment of Fusarium oxysporum f. sp. cucumerinum CRB-1


text: Indian Agricultural Research Institute

characteristic: INSDC center name

text: live

text: Generic

text: vegetative (asexual stage)

text: Fusarium oxysporum f. sp. cucumerinum

text: asexual (sexual form is unknown)

text: CRS (pathogenic group III)

text: mycelium

text: RAPD fragment of Fusarium oxysporum f. sp. cucumerinum CRS


text: Indian Agricultural Research Institute

characteristic: INSDC first public

text: live

text: Generic

text: vegetative (asexual stage)

text: Fusarium oxysporum f. sp. cucumerinum

text: asexual (sexual form is unknown)

text: CSWB-3 (pathogenic group I)

text: mycelium

text: RAPD fragment of Fusarium oxysporum f. sp. cucumerinum CSWB-3


text: University of Cambridge

characteristic: INSDC last update

text: live

text: Generic

text: Drosophila melanogaster


text: University Miguel Hernandez

text: live

characteristic: INSDC status

text: Generic

characteristic: cell type

text: Km3 Metagenomic library 3010m deep-Mediterranean Sea, Ionian Km3 Station (Italy). Fosmid DNA library prepared from marine small plankton, between 5um and 0.2um. Sample date=17th-Nov-2004. Coordinates: 36 29 98 North 15 39 97 East, Temperature: 13.93 C. Salinity: 38.75 PSU

text: marine metagenome

text: Km3-3010m-Ionian Sea


text: University of Pittsburgh School of Medicine

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: AAV2-ISceI.AO3 or AAV8-ISceI.AO3 vector produced from recombinant adeno-associated virus (rAAV) vector plasmid, pAAV-ISceI.AO3 (accession number EU022316), was injected into adult C57BL/6 or DNA-PKcs-deficient C57BL/6 SCID male mice via the tail vein (for rAAV8 vector) or the portal vein (for rAAV2 vector). rAAV provirus genome libraries were prepared from liver, skeletal muscle and heart tissues harvested 6 weeks post-injection.

text: adult

text: liver

text: Mus musculus

text: male

text: liver

text: recombinant adeno-associated virus

characteristic: vector


text: live

text: Generic

characteristic: description

text: adult

text: heart

text: Mus musculus

characteristic: organism

text: male

text: heart

characteristic: title

text: recombinant adeno-associated virus


text: live

text: Generic

text: adult

text: muscle

text: Mus musculus

text: male

text: muscle

characteristic: tissue-type

text: recombinant adeno-associated virus


text: UAM-Iztapalapa

text: live

text: Generic

characteristic: cultivar

text: Psidium guajava

text: leaf

text: P. guajava leaf, RAPD-1, pCR2.1-TOPO


text: UAM-Iztapalapa

text: live

text: Generic

text: Psidium guajava

text: leaf

text: P. guajava leaf, RAPD-2, pCR2.1-TOPO


text: UAM-Iztapalapa

text: live

text: Generic

text: Psidium guajava

text: leaf

text: P. guajava leaf, RAPD-3, pCR2.1-TOPO


text: UAM-Iztapalapa

text: live

text: Generic

text: Psidium guajava

text: leaf

text: P. guajava leaf, RAPD-4, pCR2.1-TOPO


text: UAM-Iztapalapa

text: live

text: Generic

text: Psidium guajava

text: leaf

text: P. guajava leaf, RAPD-5, pCR2.1-TOPO


text: Harvard University

text: live

text: Generic

text: Dromaius novaehollandiae

text: Emu BAC Library


text: Manitoba Institute of Cell Biology, Universitry of Manitoba

text: live

text: Generic

text: Embryonic stem cell

text: D3H (J1 subclone)

characteristic: cell-line

text: Mus musculus

text: Male

text: 129 sv

characteristic: strain

text: Retroviral insertion mutagen


characteristic: External Id

text: Cornell University

text: live

text: Generic

text: Heinz 1706

text: E. coli

characteristic: lab-host

text: Solanum lycopersicum

text: MboI

text: Tomato MboI BAC Library

text: BAC


text: Institut National de la Recherche Agronomique

characteristic: INSDC center name

text: live

text: Generic

text: one end sequence

text: Staphylococcus xylosus

text: Staphylococcus xylosus S04009 subtracted library


characteristic: INSDC first public

text: live

text: Generic

text: one end sequence

text: Staphylococcus xylosus

text: Staphylococcus xylosus 00-1747 subtracted library


characteristic: INSDC last update

text: live

text: Generic

text: one end sequence

text: Staphylococcus xylosus

text: Staphylococcus xylosus S04002 subtracted library


text: University of Milan

text: live

characteristic: INSDC status

text: Generic

text: Dirofilaria immitis

text: Wolbachia endosymbiont of Dirofilaria immitis

text: Wolbachia of Dirofilaria immitis Library

text: Plasmid

text: p-SmartC


text: live

text: Generic

text: Embryonic stem cell

characteristic: cell type

text: E14Tg2a.4, stain/genetic background: 129P2/OlaHsd

text: Mus musculus

text: 129 sv

text: Retroviral insertion mutagen


text: The University of Arizona

text: live

text: Generic

characteristic: NCBI submission model

text: Embryo

characteristic: dev-stage

text: DH10B T1 phage resistant

text: Drosophila americana

text: BamHI

text: BamHI

text: Embryo

text: Plasmid


text: The University of Arizona

text: live

text: Generic

characteristic: NCBI submission package

text: 0-12 hr

text: DH10B T1 phage resistant

text: Drosophila ananassae

text: BamHI

text: BamHI

text: embryo

text: Plasmid

characteristic: vector


text: The University of Arizona

text: live

text: Generic

text: 0-6 hr embryo

text: DH10B T1 phage resistant

text: Drosophila erecta

characteristic: organism

text: HindIII

text: HindIII

text: TSC line#14021-0224.01

text: embryos

text: Plasmid


text: The University of Arizona

text: live

text: Generic

text: 0-24hr post oviposition

text: DH10B T1 phage resistant

text: Drosophila mojavensis

text: BamHI

text: BamHI

text: CI-12 IB4

text: embryo

characteristic: title

text: Plasmid


text: The University of Arizona

text: live

text: Generic

text: DH10B T1 phage resistant

text: Drosophila sechellia

text: HindIII

text: HindIII

text: embryos

characteristic: tissue-type

text: Plasmid


text: The University of Arizona

text: live

text: Generic

text: 0-24 hr post oviposition

text: DH10B T1 phage resistant

text: Drosophila virilis

text: BamHI

text: BamHI

text: embryos by Tom Watts

text: Plasmid


text: The University of Arizona

text: live

text: Generic

text: DH10B T1 phage resistant

text: Drosophila novamexicana

text: HindIII

text: HindIII

text: embryo

text: Plasmid


text: The University of Arizona

text: live

text: Generic

text: embryo

text: DH10B T1 phage resistant

text: Drosophila grimshawi

text: HindIII

text: HindIII

text: embryo


text: The University of Arizona

text: live

text: Generic

text: embryo

text: DH10B T1 phage resistant

text: Drosophila hydei

text: HindIII

text: HindIII

text: embryo


text: The University of Arizona

text: live

text: Generic

text: embryo

text: DH10B T1 phage resistant

text: Drosophila mercatorum

text: HindIII

text: HindIII

text: embryo


text: The University of Arizona

text: live

text: Generic

text: embryo

text: DH10B T1 phage resistant

text: Drosophila persimilis

text: HindIII

text: HindIII

text: embryo


text: The University of Arizona

text: live

text: Generic

text: embryo

text: DH10B T1 phage resistant

text: Drosophila willistoni

text: HindIII

text: HindIII

text: embryo


text: The University of Arizona

text: live

text: Generic

text: embryo

text: DH10B T1 phage resistant

text: Drosophila yakuba

text: HindIII

text: HindIII

text: embryo


text: The University of Arizona

text: live

text: Generic

text: embryo

text: DH10B T1 phage resistant

text: Drosophila arizonae

text: HindIII

text: HindIII

text: embryo


text: The University of Arizona

text: live

text: Generic

text: embryo

text: DH10B T1 phage resistant

text: Drosophila busckii

text: HindIII

text: HindIII

text: embryo


text: The University of Arizona

text: live

text: Generic

text: embryo

text: DH10B T1 phage resistant

text: Drosophila littoralis

text: HindIII

text: HindIII

text: embryo


text: The University of Arizona

text: live

text: Generic

text: embryo

text: DH10B T1 phage resistant

text: Drosophila repleta

text: HindIII

text: HindIII

text: embryo


text: The University of Arizona

text: live

text: Generic

text: embryo

text: DH10B T1 phage resistant

text: Drosophila simulans

text: HindIII

text: HindIII

text: embryo


text: UCSF Department of Urology

text: live

text: Generic

text: This library was constructed from MCF7 breast cancer cell line by Amplicon Express (http://www.genomex.com) using their standard procedure.

characteristic: description

text: Brain

characteristic: organ

text: Homo sapiens

text: HindIII

text: Recurrent glioblastoma

text: BAC library from the recurrent glioblastoma sample AA9


text: UCSF Department of Urology

text: live

text: Generic

text: This library was constructed from B421 tumor sample by Amplicon Express (http://www.genomex.com) using their standard procedure. Patient underwent 4 months of chemotherapy prior to surgery (CMF), no recurrence till patient's death from non-cancer related causes.

text: Breast

text: Homo sapiens

text: HindIII

text: Primary tumor

text: BAC library from breast tumor sample B421


text: Stanford University

text: live

text: Generic

text: mix background A188/W23/B73/K55

characteristic: cultivar

text: MightyMu_ACT is a 5.3 kb, modified maize Mu1 transposon designed for activation tagging and to allowed plasmid rescue from total genomic DNA. Total leaf DNA was double digested using BamHI and BglII, and ligated to form circular plasmids. DH10B cells were transformed and then screened on LB plates with ampicillin.

text: adult

text: Leaf

text: Zea mays

text: BamHI

text: BglII

text: Leaf

text: GNK

text: plasmid

text: MightyMu_ACT (engineered from pBluescript)


characteristic: External Id

text: Stanford University

text: live

text: Generic

text: mix background A188/W23/B73/K55

text: adult

text: Leaf

text: Zea mays

text: BamHI

text: BglII

text: Leaf

text: GNM

text: plasmid


text: Stanford University

text: live

text: Generic

text: mix background A188/W23/B73/K55

text: adult

characteristic: lab-host

text: Leaf

text: Zea mays

text: BamHI

text: BglII

text: Leaf

text: GNN

text: plasmid


text: Southern Illinois University at Carbondale and The Institute for Genomic Research

characteristic: INSDC center name

text: live

text: Generic

text: Forrest

text: Soybean (Glycine max (L.) Merr.) cv. Forrest seeds were grown in greenhouse for fourteen days. Nuclei were isolated and embedded in agarose, restriction digested with Hind III BamH1 or EcoRI, large size DNA fragments were ligated in vector V41 (pCLD04541) and electro transformed in DH10a cells. About 90,000 clones from BAC libraries were fingerprinted with HindIII and Hae III. Version 2 (automatic build) Contigs were built from 78,001 fingerprints. Contigs were manually examined to find the best non redundant tile path through the contigs representing about 13,000 clones. The clones were end sequenced.

text: Leaves

text: Glycine max

text: LargeInsertSoybeanGenLib

text: pCLD04541 (pBELOBAC11 EcorI clones)


text: UCSF Department of Urology

characteristic: INSDC first public

text: live

text: Generic

text: This library was constructed from s104 primary breast tumor sample by Dr. M. Nefedov (Dr. Peter de Jong's laboratory). The patient did not receive chemo- or radiotherapy and did not have recurrence for 10 years after surgery.

text: Breast

text: Homo sapiens

text: HindIII

text: Primary tumor

text: BAC library from the primary breast tumor sample s104


text: UCSF Department of Urology

characteristic: INSDC last update

text: live

text: Generic

characteristic: cell-line

text: This library was constructed from SK-BR-3 cell line by Dr. M. Nefedov (Dr. Peter de Jong's laboratory).

text: Breast

text: Homo sapiens

text: HindIII

text: Adenocarcinoma, metastasis (pleural effusion)

text: BAC library from the breast cell line SK-BR-3


text: UCSF Department of Urology

text: live

characteristic: INSDC status

text: Generic

text: This library was constructed from 860 primary ovarian tumor sample by Dr. M. Nefedov (Dr. Peter de Jong's laboratory). The patient did not receive chemo- or radiotherapy prior to surgery.

text: Ovary

text: Homo sapiens

text: HindIII

text: Ovarian carcinoma

text: BAC library from the primary ovarian tumor sample 860


text: UCSF Department of Urology

text: live

text: Generic

characteristic: NCBI submission model

text: This library was constructed from the prostate metastasis sample 25 by Amplicon Express (http://www.genomex.com). Patient was treated with hormone-ablation and radiotherapy.

text: Prostate

text: Homo sapiens

text: HindIII

text: Metastasis

text: BAC library from the prostate metastasis sample 25


text: UCSF Department of Urology

text: live

text: Generic

text: This library was constructed from BT-474 cell line by Dr. M. Nefedov (Dr. Peter de Jong's laboratory).

text: Breast

text: Homo sapiens

text: HindIII

text: ductal carcinoma

text: BAC library from the breast cell line BT-474


text: University of Cologne

text: live

text: Generic

text: sperm

characteristic: cell type

text: Cottus perifretum

text: EcoRI

text: River Wahnbach

characteristic: strain

text: Cottus perifretum pZero library


text: Universidade Federal de Uberlandia

text: live

text: Generic

characteristic: NCBI submission package

text: Caryocar brasiliense

text: RAPD marker from pequi without thorn


text: Universidade Federal de Uberlandia

text: live

text: Generic

text: Caryocar brasiliense

characteristic: organism

text: RAPD marker from pequi with thorn


text: University of Toronto

text: live

text: Generic

text: Embryonic Stem Cell

text: Mus musculus

text: Male

characteristic: sex

characteristic: title

text: Gene trap insertion mutagen

characteristic: vector


text: Lawrence Berkeley National Laboratory

text: live

text: Generic

text: Drosophila melanogaster was mutagenized by remobilization of the piggyBac vector pBac{GAL4D,EYFP} (Horn et al. 2003 Genetics 163: 647-661) (FlyBase ID Fbtp0017476) as described by Hacker et al. 2003 (Proc Natl Acad Sci USA 100: 7720-7725). Each strain in the collection has an independent insertion of the mutator transposon and the name of each strain begins with the letters PL. Genomic DNA was prepared from samples from each PL strain, DNA segments flanking the transposon insertion sites were amplified by inverse PCR, and the inverse PCR products were sequenced as described by Bellen et al. 2004 (Genetics 167: 761-781).

characteristic: description

text: Drosophila melanogaster

text: Drosophila melanogaster PL strains, transposon insertion flanking sequences


text: Chungnam National University

text: live

text: Generic

text: Sus scrofa

text: Korean native pig

text: Korean native pig BAC end sequence


text: University of California Santa Cruz

text: live

text: Generic

text: Metagenomic library from North Atlantic station SJ060903 (12.257 N 56.146 W) on 27-Jun-2006

text: uncultured marine microorganism


text: University of California Santa Cruz

text: live

text: Generic

text: Metagenomic library from North Atlantic station SJ060912 (14.976 N 31.958 W) on 07-Jul-2006

text: uncultured marine microorganism


text: Yamaguchi University

text: live

text: Generic

text: Hagi

characteristic: isolate

text: Plasmodiophora brassicae

text: Plasmodiophora brassicae Hagi DNA


characteristic: External Id

text: National Institute of Agrobiological Sciences

text: live

text: Generic

text: Sus scrofa

text: Sus scrofa shotgun BAC sequence


text: USDA-ARS Western Regional Research Center

text: live

text: Generic

characteristic: cultivar

text: Library sequences were extracted from ABI 3730XL tracefiles. Quality Read Lengths from sequences were extracted with Phred program. File trimming based on sequence mark-up done with cross_match program, minor vector sequences remained. Quality files and additional information available from project site at: http://wheat.pw.usda.gov/bEST

text: Seedlings

characteristic: dev-stage

text: Escherichia coli TransforMax EPI300

characteristic: lab-host

text: Brachypodium distachyon

text: HindIII

text: Seedlings

characteristic: tissue-type

text: Brachypodium distachyon Bd21 HindIII BAC library

text: BAC


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Seedlings

text: Escherichia coli TransforMax EPI300

text: Brachypodium distachyon

text: BamHI

text: Seedlings

text: Brachypodium distachyon Bd21 BamHI BAC library

text: BAC


text: CSIRO Livestock Industries

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: White blood cells

characteristic: cell type

text: PCR products from site specific genome amplification

text: Adult

text: Ovis aries

text: Awassi

text: Blood

text: Awassi PCR library


text: CSIRO Livestock Industries

text: live

text: Generic

characteristic: NCBI submission package

text: White blood cells

text: Adult

text: Ovis aries

characteristic: organism

text: Gulf Coast Native

characteristic: strain

text: Blood

text: Gulf Coast Native PCR library


text: CSIRO Livestock Industries

text: live

text: Generic

text: White blood cells

text: Adult

text: Ovis aries

text: Katahdin

text: Blood

text: Katahdin PCR library

characteristic: title


text: CSIRO Livestock Industries

text: live

text: Generic

text: White blood cells

characteristic: description

text: Adult

text: Ovis aries

text: Laucaune

text: Blood

text: Laucaune PCR library


text: CSIRO Livestock Industries

text: live

text: Generic

text: White blood cells

text: Adult

text: Ovis aries

text: Merino

text: Blood

text: Merino PCR library


text: CSIRO Livestock Industries

text: live

text: Generic

text: White blood cells

text: Adult

text: Ovis aries

text: Merino

text: Blood

text: Merino Pool PCR library


text: CSIRO Livestock Industries

text: live

text: Generic

text: White blood cells

text: Adult

text: Ovis aries

text: Poll Dorset

text: Blood

text: Poll Dorset PCR library


text: CSIRO Livestock Industries

text: live

text: Generic

text: White blood cells

text: Adult

text: Ovis aries

text: Poll Dorset Pool

text: Blood

text: Poll Dorset Pool PCR library


text: CSIRO Livestock Industries

text: live

text: Generic

text: White blood cells

text: Adult

text: Ovis aries

text: Red Masai

text: Blood

text: Red Masai PCR library


text: CSIRO Livestock Industries

text: live

text: Generic

text: White blood cells

text: Adult

text: Ovis aries

text: Romney

text: Blood

text: Romney PCR library


text: CSIRO Livestock Industries

text: live

text: Generic

text: White blood cells

text: Adult

text: Ovis aries

text: Texel

text: Blood

text: Texel PCR library


text: CSIRO Livestock Industries

text: live

text: Generic

text: White blood cells

text: Adult

text: Ovis aries

text: Blood

text: TRP PCR library


text: Yeungnam University

text: live

text: Generic

text: Leucocyte

text: Adult

text: Bos taurus

text: HindIII

text: Male

characteristic: sex

text: Korean Cattle (Hanwoo)

text: Blood

text: HW-YUBAC

text: BAC

characteristic: vector


characteristic: External Id

text: Agricultural Biotechnology Center

text: live

text: Generic

text: Jemalong (A17)

characteristic: cultivar

text: Construction of a bacterial artificial chromosome library of Medicago truncatula and identification of clones containing ethylene-response genes. Theor Appl Genet (1999) 98: 638-646 Y.-W.,Nam; R.V.,Penmetsa; G.,Endre; P.,Uribe; D.,Kim; D.R.,Cook

text: Leaf

characteristic: organ

text: Medicago truncatula

text: HindIII

text: HindIII

text: Hermaphrodite

text: mth2 BAC library

text: BAC


text: IFREMER

text: live

text: Generic

text: SSH between the highly pathogenic strain SFn1 (Tester) and the non-pathogenic strain SFn118 (Driver).

text: Vibrio nigripulchritudo

text: Suppressive Subtractive Hybridization (SSH) library


text: IFREMER

text: live

text: Generic

text: plasmid pSFn1. SSH between the highly pathogenic strain SFn1 (Tester) and the non-pathogenic strain SFn118 (Driver).

text: Vibrio nigripulchritudo

text: plasmid pSFn1 Suppressive Subtractive Hybridization (SSH) library


text: International Institute of Tropical Agriculture (IITA)

text: live

text: Generic

text: Musa acuminata

text: Musa acuminata acc. Calcutta 4


text: live

text: Generic

text: Musa balbisiana

text: Musa balbisiana acc. MPL


text: live

text: Generic

text: Musa ABB Group

text: Musa spp. (cooking banana Bluggoe)


text: live

text: Generic

text: Musa AAB Group

text: Musa spp. (Plantain, Agbagba)


text: live

text: Generic

text: Musa AB Group

text: Musa spp. var. Nepoovan


text: CSIR Biosciences

text: live

text: Generic

text: Stenocarpella maydis

text: Stenocarpella maydis genomic sequence


text: University of California, Davis

text: live

text: Generic

text: Sequenced introns from various tomato genes for paper 'Diversity in Conserved Genes in Tomato', BMC Genomics

text: Solanum lycopersicum

text: Tomato Genome SNP,Indel Search Library


text: Purdue University

text: live

text: Generic

text: DNA sequence obtained from PCR products amplified from W22 genomic DNA using gene target - specific primers. The PCR product was sequenced from both the 5' and 3' ends.

text: Zea mays

text: Maize TILLING Project Target Sequences W22


text: University of California Santa Cruz

text: live

text: Generic

text: Viral concentrates were generated from surface water samples collected at 9 stations along the length of the Chesapeake Bay and pooled into a single concentrate. Metagenomic subclone library constructed after LASL amplification of pooled viral DNA. Subclone plasmids were amplified via TempliPhi (Amersham, Piscataway, NJ) prior to dye terminator sequencing and separation via capillary electrophoresis.

text: uncultured marine virus

text: Chesapeake Bay viral metagenome library_A (Sept. 2002)

text: Plasmid

text: pSmartHCKan (Lucigen, Middleton, WI)


text: Tulane University Health Science Center

text: live

text: Generic

text: Cultured schistosomes were transformed with the transposon piggyBac. Genomic DNA was extracted from the transposon-transformed worms. Anchored PCRs were carried out using the genomic DNA as the template and primers specific (1) for the piggyBac transgene construct and (2) the endogenous schistosome retrotransposon SR1. The resulting PCR products were cloned into the plasmid cloning vector TOPO XL to produce the SR1-piggyBac partial genomic DNA library.

text: Schistosomulum

characteristic: dev-stage

text: Biomphalaria glabrata

characteristic: lab-host

text: Schistosoma mansoni

text: Vector with TA overhang

text: Vector with TA overhang

text: Mixed sex, males and females

text: Puerto Rican

text: homogenized whole worm extract

characteristic: tissue-type

text: Plasmid

text: TOPO XL


text: Tulane University Health Science Center

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Cultured schistosomes were transformed with the transposon piggyBac. Genomic DNA was extracted from the transposon-transformed worms. Anchored PCRs were carried out using the genomic DNA as the template and primers specific (1) for the piggyBac transgene construct and (2) the endogenous schistosome retrotransposon SR2. The resulting PCR products were cloned into the plasmid cloning vector TOPO XL to produce the SR2-piggyBac partial genomic DNA library.

text: Schistosomulum

text: Biomphalaria glabrata

text: Schistosoma mansoni

text: Vector with TA overhang

text: Vector with TA overhang

text: Mixed sex, males and females

text: Puerto Rican

text: homogenized whole worm extract

text: Plasmid

text: TOPO XL


text: Tulane University Health Science Center

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Cultured schistosomes were transformed with the transposon piggyBac. Genomic DNA was extracted from the transposon-transformed worms. Anchored PCRs were carried out using the genomic DNA as the template and primers specific (1) for the piggyBac transgene construct and (2) the endogenous schistosome retrotransposon Boudicca. The resulting PCR products were cloned into the plasmid cloning vector TOPO XL to produce the Boudicca-piggyBac partial genomic DNA library.

text: Schistosomulum

text: Biomphalaria glabrata

text: Schistosoma mansoni

text: Vector with TA overhang

text: Vector with TA overhang

text: Mixed sex, males and females

text: Puerto Rican

text: homogenized whole worm extract

text: Plasmid

text: TOPO XL


text: The University of Arizona

text: live

text: Generic

characteristic: NCBI submission package

text: embryo

text: DH10B T1 phage resistant

text: Drosophila albomicans

characteristic: organism

text: HindIII

text: HindIII

text: embryo