BLun

text: CNRS

text: live

text: Generic

text: Total RNA was extracted using Ambion’s miRVana kit, then small (18-30 nt long) RNAs were purified on a denaturing 15 % acrylamide gel, from 24 to 69 μg total RNA per sample. RNAs were eluted from the acrylamide bands overnight in 0.3 M NaCl, then ethanol/glycogen-precipitated. Half of each sample was oxidized (12 μL RNA; 8 μL 5X borate buffer (148 mM borax, 148 mM boric acid, ph=8.6); 5 μL freshly prepared 200 mM NaIO4 ; 15 μL water) for 30 minutes at 25 degrees C, then precipitated with sodium acetate, ethanol and glycogen; the other half was not treated. These RNA samples were then ligated to the preadenylated 3 ́ adapter (5 ́-rAppdCdT dGdTdA dGdGdC dAdCdC dAdTdC dAdAdT ddC-3 ́): 1 μL 100 μM preadenylated 3 ́ adapter 2 μL 10X ligation buffer (500 mM Tris pH=7.5; 100 mM MgCl2 ; 600 μg/mL BSA; 100 mM DTT) 2 μL DMSO 1 μL RNAsin (Promega; 40 U/μL) 2 μL truncated Rnl2 ligase (“Rnl2(1-249”, described in Ho, C. K., Wang, L. K., Lima, C. D. & Shuman, S. (2004) Structure and mechanism of RNA ligase. Structure 12, 327-339) 12 μL RNA were mixed and incubated at 25 degrees C for approximately 24h, then ligation products were purified on a denaturing 15 % acrylamide gel. RNAs were eluted from the acrylamide bands overnight in 0.3 M NaCl, then ethanol/glycogen-precipitated, and ligated to the 5 ́ adapter (5 ́-rGrUrU rCrArG rArGrU rUrCrU rArCrA rGrUrC rCrGrA rCrGrA rUrC-3 ́): 100 pmol 5 ́ adapter 2 μL 10X T4 RNA ligase buffer (Ambion) 2 μL DMSO 2 μL RNA ligase (Ambion) 13 μL RNA were incubated 6h at 25 degrees C, then purified on a denaturing 15 % acrylamide gel. RNAs were eluted from the acrylamide bands overnight in 0.3 M NaCl, then ethanol/glycogen-precipitated, and reverse-transcribed: 20 μL RNA + 1 μL 100 μM RT primer (5 ́-dAdTdT dGdAdT dGdGdT dGdCdC dTdAdC dAdG-3 ́) were denatured 2 min. at 72 degrees C, then annealed 2 min. at room temperature, then left a few minutes on ice 0.84 μL DTT 3.36 μL water 4.2 μL dNTP mix (10 mM each) 8.4 μL 5X first strand buffer (Invitrogen) were added, then each mix was splitted into two tubes: “+RT” (were 2 μL Superscript III, Invitrogen, were added) and “-RT” (were 2 μL water were added), then the reactions were incubated 1 hour at 50 degrees C, and the enzyme was inactivated 15 min. at 70 degrees C. 2 μL of each reaction were used as templates for a PCR amplification (with 25 pmol of each primer: 5 ́- dAdAdT dGdAdT dAdCdG dGdCdG dAdCdC dAdCdC dGdAdC dAdGdG dTdTdC dAdGdA dGdTdT dCdTdA dCdAdG dTdCdC dGdA -3 ́ and 5 ́-dCdAdA dGdCdA dGdAdA dGdAdC dGdGdC dAdTdA dCdGdA dAdTdT dGdAdT dGdGdT dGdCdC dTdAdC dAdG-3 ́, and one PuReTaq Ready-To-Go PCR bead, GE Healthcare, in 25 μL): 2 min. at 94 degrees C, 5 times (15 s. at 94 degrees C, 30 s. at 54 degrees C, 30 s. at 72 degrees C), 17 times (15 s. at 94 degrees C, 30 s. at 60 degrees C, 30 s. at 72 degrees C), then 2 min. at 72 degrees C. The RT-PCR products were purified on a 2 % NuSieve GTG agarose gel, and extracted with the following protocol: the agarose bands were melted in 0.4 M NaCl (100 μL per 100 mg agarose) for 15 min. at 70 degrees C; the solution was extracted with 1 volume of phenol (buffered at pH=7.9) at room temperature, then 1 volume of phenol/chloroform/isoamylalcohol (25/24/1 v/v/v), then 1 volume chloroform/isoamylalcohol (24/1 v/v) then precipitated with 2.5 volumes ethanol. 2 pM solutions of the resulting libraries were seeded on each Solexa lane, at the Deep Sequencing Core facility of UMass Medical School (Worcester, MA, USA).

text: Nematostella vectensis

text: Generic sample from Nematostella vectensis


status: PUBLIC

characteristic: External Id

text: Emory University

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

characteristic: INSDC secondary accession

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

characteristic: SRA accession

text: lymphogranuloma venerum (LGV) strain, termed L2c, isolated from a man with severe proctitis.

characteristic: description

text: Chlamydia trachomatis L2c

characteristic: organism

tag: attribute

characteristic: strain

text: Chalmydia trachomatis L2c

characteristic: title


base

text: Cornell University

text: live

text: Generic

text: Zea mays B73 inbred seedlings were grown under an 80:20 mix of metal halide: capsylite halogen lamps at light intensity of 550 mol/m2/sec, 12:12 L/D, 31*C L/22*C D, 50% relative humidity. Tissue was collected from leaf three at 9 DAP three hours into the L period from the basal segment (1cm above the leaf three ligule). Tissue was pooled from 15 seedlings.

text: Zea mays subsp. mays

text: B73 basal sample


text: Cornell University

text: live

text: Generic

text: Zea mays B73 inbred seedlings were grown under an 80:20 mix of metal halide: capsylite halogen lamps at light intensity of 550 mol/m2/sec, 12:12 L/D, 31*C L/22*C D, 50% relative humidity. Tissue was collected from leaf three at 9 DAP three hours into the L period from the transitional segment (1cm below the leaf two ligule). Tissue was pooled from 15 seedlings.

text: Zea mays subsp. mays

text: B73 transitional sample


text: Cornell University

text: live

text: Generic

text: Zea mays B73 inbred seedlings were grown under an 80:20 mix of metal halide: capsylite halogen lamps at light intensity of 550 mol/m2/sec, 12:12 L/D, 31*C L/22*C D, 50% relative humidity. Tissue was collected from leaf three at 9 DAP three hours into the L period from the maturing segment (4cm above the leaf two ligule). Tissue was pooled from 15 seedlings.

text: Zea mays subsp. mays

text: B73 maturing sample


tip

text: Cornell University

text: live

text: Generic

text: Zea mays B73 inbred seedlings were grown under an 80:20 mix of metal halide: capsylite halogen lamps at light intensity of 550 mol/m2/sec, 12:12 L/D, 31*C L/22*C D, 50% relative humidity. Tissue was collected from leaf three at 9 DAP three hours into the L period from the mature segment (1cm below the leaf three tip). Tissue was pooled from 15 seedlings.

text: Zea mays subsp. mays

text: B73 mature sample


bundle-sheath

text: Cornell University

text: live

text: Generic

text: Zea mays B73 inbred seedlings were grown under an 80:20 mix of metal halide: capsylite halogen lamps at light intensity of 550 mol/m2/sec, 12:12 L/D, 31*C L/22*C D, 50% relative humidity. LCM was performed on pre-fixed and embedded B73 leaf tip tissue using the PixCell IIE and the Veritas laser microdissection systems. Individual mesophyll and bundle sheath cells were collected on the Capsure HS LCM caps

text: Zea mays subsp. mays

text: B73 bundle-sheath sample


mesophyll

text: Cornell University

text: live

text: Generic

text: Zea mays B73 inbred seedlings were grown under an 80:20 mix of metal halide: capsylite halogen lamps at light intensity of 550 mol/m2/sec, 12:12 L/D, 31*C L/22*C D, 50% relative humidity. LCM was performed on pre-fixed and embedded B73 leaf tip tissue using the PixCell IIE and the Veritas laser microdissection systems. Individual mesophyll and bundle sheath cells were collected on the Capsure HS LCM caps.

text: Zea mays subsp. mays

text: B73 mesophyll sample


text: HudsonAlpha

text: live

text: Generic

text: ChIP-seq was used to find the binding sites for the transcription factor Bcl3 in hESC cells as described in the Study category for this entry. The replicate number indicates Rep1. For more details please contact the Myers Lab at the HudsonAlpha Institute for Biotechnology.

text: Homo sapiens


text: HudsonAlpha

text: live

text: Generic

text: ChIP-seq was used to find the binding sites for the transcription factor Bcl3 in hESC cells as described in the Study category for this entry. The replicate number indicates For more details please contact the Myers Lab at the HudsonAlpha Institute for Biotechnology.

text: Homo sapiens

text: Generic sample from Homo sapiens


text: HudsonAlpha

text: live

text: Generic

text: ChIP-seq was used to find the binding sites for the transcription factor Kaiso in K562 cells as described in the Study category for this entry. The replicate number indicatesFor more details please contact the Myers Lab at the HudsonAlpha Institute for Biotechnology.

text: Homo sapiens

text: Generic sample from Homo sapiens


text: HudsonAlpha

text: live

text: Generic

text: ChIP-seq was used to find the binding sites for the transcription factor Kaiso in K562 cells as described in the Study category for this entry. The replicate number indicates it is Rep 5. For more details please contact the Myers Lab at the HudsonAlpha Institute for Biotechnology.

text: Homo sapiens

text: Generic sample from Homo sapiens


text: Mattick Laboratory, Institute for Molecular Bioscience, University of Queensland

text: live

text: Generic

text: granulocyte

characteristic: cell type

text: Mus musculus

text: mouse primary granulocyte nuclei


text: live

text: Generic

characteristic: cell line

text: Homo sapiens

text: 10 to 14

characteristic: passages

text: monocytic leukemia cell line


text: Antonio Prudente Fundation

text: live

text: Generic

text: Homo sapiens

text: HB4a C5.2 800k dataset


text: UCSD/Bioengineering

text: live

text: Generic

text: Homo sapiens

text: UCSD Cpg30k MIP Human Targeted Bisulfited Paired End Sequencing


text: Human Genetics, NCMLS

text: live

text: Generic

text: 4A4 against p63

characteristic: antibody

text: primary cells

text: keratinocytes

text: Homo sapiens

text: primary keratinocyte

text: epidermis

characteristic: tissue


characteristic: External Id

text: Riitta Lahesmaa, Turku Centre for Biotechnology, University of Turku

characteristic: INSDC center name

text: live

text: Generic

text: CD4+ T cells

text: Homo sapiens

text: human cord blood CD4+ cell, 0h

text: cord blood

text: Cord blood CD4+_0h


characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: CD4+ T cells

text: Homo sapiens

text: human cord blood CD4+ cell, activated, 1h

text: cord blood

text: Cord blood CD4+_Act_1h


characteristic: INSDC secondary accession

text: live

characteristic: INSDC status

text: Generic

text: CD4+ T cells

text: Homo sapiens

text: human cord blood CD4+ cell, activated and IL-4 treated, 1h

text: cord blood

text: Cord blood CD4+_Act+IL-4_1h


text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: CD4+ T cells

text: Homo sapiens

text: human cord blood CD4+ cell, activated, 4h

text: cord blood

text: Cord blood CD4+_Act_4h


text: live

text: Generic

characteristic: SRA accession

text: CD4+ T cells

text: Homo sapiens

text: human cord blood CD4+ cell, activated and IL-4 treated, 4h

text: cord blood

text: Cord blood CD4+_Act+IL-4_4h


text: MIT

text: live

text: Generic

characteristic: chip antibody

text: Mus musculus

characteristic: organism

text: hepatocytes

characteristic: sample

text: liver hepatocytes

text: p300 liver ChIPseq


text: MIT

text: live

text: Generic

text: C/EBPalpha

text: Mus musculus

text: hepatocytes

text: liver hepatocytes

characteristic: strain

text: CEBPa liver ChIPseq

characteristic: title


text: MIT

text: live

text: Generic

text: Mus musculus

text: hepatocytes

text: liver hepatocytes

text: FOXA2 liver ChIPseq


text: MIT

text: live

text: Generic

text: Mus musculus

text: hepatocytes

text: liver hepatocytes

text: FOXA1 liver ChIPseq


text: MIT

text: live

text: Generic

text: Mus musculus

text: hepatocytes

text: liver hepatocytes

text: E2F4 liver ChIPseq


text: MIT

text: live

text: Generic

text: Mus musculus

text: fully differentiated 3T3-L1 cells

text: differentiated 3T3-L1 cells

text: p300 3T3-L1 ChIPseq


text: MIT

text: live

text: Generic

text: C/EBPalpha

text: Mus musculus

text: fully differentiated 3T3-L1 cells

text: differentiated 3T3-L1 cells

text: CEBPa 3T3-L1 ChIPseq


text: MIT

text: live

text: Generic

text: Mus musculus

text: fully differentiated 3T3-L1 cells

text: differentiated 3T3-L1 cells

text: E2F4 3T3-L1 ChIPseq


text: MIT

text: live

text: Generic

text: Mus musculus

text: cerebellum

text: cerebellum

text: Sirt1 cerebellum ChIPseq


text: MIT

text: live

text: Generic

text: Mus musculus

text: liver hepatocytes

text: liver hepatocytes

text: Sample control reads liver ChIPseq


text: MIT

text: live

text: Generic

text: Mus musculus

text: fully differentiated 3T3-L1 cells

text: differentiated 3T3-L1 cells

text: Sample control reads 3T3-L1 ChIPseq


text: MIT

text: live

text: Generic

text: Mus musculus

text: cerebellum

text: cerebellum

text: Sample control reads cerebellum ChIPseq


text: Plant Biology, Carnegie Institute for Science

text: live

text: Generic

text: TAP

characteristic: medium

text: Chlamydomonas reinhardtii

text: Chlamydomonas cultured cells


text: live

text: Generic

text: TAP -S

text: Chlamydomonas reinhardtii

text: Chlamydomonas cultured cells

text: 6 hours

characteristic: sulfur deprivation time


text: University of California - Davis, Drosophila Population Genomics Project

text: live

text: Generic

text: A Drosophila melanogaster genome that is isogenic for the X chromosome (extracted with a balancer) from an isofemale line established from a single inseminated female collected in Malawi, Africa.

characteristic: description

text: Drosophila melanogaster

text: Generic sample from Drosophila melanogaster


characteristic: External Id

text: University of Georgia

text: live

text: Generic

text: Persea americana

text: Generic sample from Persea americana


Prochlorococcus sp. UH18301 Sample

text: J. Craig Venter Institute

characteristic: INSDC center name

text: live

text: Generic

text: 454 Sequencing of Prochlorococcus sp. UH18301

text: Prochlorococcus sp. UH18301

text: Generic sample from Prochlorococcus sp. UH18301


text: University of East Anglia

characteristic: INSDC first public

text: live

text: Generic

text: Nicotiana benthamiana

text: Early systemic leaves of CymRSV-infected Nicotiana benthamiana

text: early systemic leaves

characteristic: tissue

text: Cymbidium Ringspot Virus (CymRSV)-derived siRNAs

text: CymRSV infection

characteristic: treatment


text: University of East Anglia

characteristic: INSDC last update

characteristic: INSDC secondary accession

text: live

characteristic: INSDC status

text: Generic

text: Nicotiana benthamiana

text: Early systemic leaves of Cym19stop-infected Nicotiana benthamiana

text: early systemic leaves

text: P19 silencing suppressor mutant Cymbidium Ringspot Virus (Cym19stop)-derived de- and re-phosphorylated siRNAs

text: Cym19stop infection


text: University of East Anglia

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

characteristic: SRA accession

text: Nicotiana benthamiana

text: early systemic leaves

text: P19 silencing suppressor mutant Cymbidium Ringspot Virus (Cym19stop)-derived siRNAs

text: Cym19stop infection


text: University of East Anglia

text: live

text: Generic

text: Nicotiana benthamiana

characteristic: organism

text: early systemic leaves

text: Cymbidium Ringspot Virus (CymRSV)-derived de-phosphorylated and re-phosphorylated siRNAs

text: CymRSV infection


text: Biochemistry and Molecular biology, Oklahoma State University

text: live

text: Generic

text: spp japonica cv. Nipponbare

characteristic: cultivar

text: Oryza sativa

text: seedlings

text: Rice degradome


text: live

text: Generic

text: A Drosophila melanogaster genome that is isogenic for chromosome 3 (extracted with a balancer) from an isofemale line established from a single inseminated female collected in Malawi, Africa.

text: Drosophila melanogaster

characteristic: title


UEun

text: CNRS

text: live

text: Generic

text: Total RNA was extracted using Ambion’s miRVana kit, then small (18-30 nt long) RNAs were purified on a denaturing 15 % acrylamide gel, from 24 to 69 μg total RNA per sample. RNAs were eluted from the acrylamide bands overnight in 0.3 M NaCl, then ethanol/glycogen-precipitated. Half of each sample was oxidized (12 μL RNA; 8 μL 5X borate buffer (148 mM borax, 148 mM boric acid, ph=8.6); 5 μL freshly prepared 200 mM NaIO4 ; 15 μL water) for 30 minutes at 25 degrees C, then precipitated with sodium acetate, ethanol and glycogen; the other half was not treated. These RNA samples were then ligated to the preadenylated 3 ́ adapter (5 ́-rAppdCdT dGdTdA dGdGdC dAdCdC dAdTdC dAdAdT ddC-3 ́): 1 μL 100 μM preadenylated 3 ́ adapter 2 μL 10X ligation buffer (500 mM Tris pH=7.5; 100 mM MgCl2 ; 600 μg/mL BSA; 100 mM DTT) 2 μL DMSO 1 μL RNAsin (Promega; 40 U/μL) 2 μL truncated Rnl2 ligase (“Rnl2(1-249”, described in Ho, C. K., Wang, L. K., Lima, C. D. & Shuman, S. (2004) Structure and mechanism of RNA ligase. Structure 12, 327-339) 12 μL RNA were mixed and incubated at 25 degrees C for approximately 24h, then ligation products were purified on a denaturing 15 % acrylamide gel. RNAs were eluted from the acrylamide bands overnight in 0.3 M NaCl, then ethanol/glycogen-precipitated, and ligated to the 5 ́ adapter (5 ́-rGrUrU rCrArG rArGrU rUrCrU rArCrA rGrUrC rCrGrA rCrGrA rUrC-3 ́): 100 pmol 5 ́ adapter 2 μL 10X T4 RNA ligase buffer (Ambion) 2 μL DMSO 2 μL RNA ligase (Ambion) 13 μL RNA were incubated 6h at 25 degrees C, then purified on a denaturing 15 % acrylamide gel. RNAs were eluted from the acrylamide bands overnight in 0.3 M NaCl, then ethanol/glycogen-precipitated, and reverse-transcribed: 20 μL RNA + 1 μL 100 μM RT primer (5 ́-dAdTdT dGdAdT dGdGdT dGdCdC dTdAdC dAdG-3 ́) were denatured 2 min. at 72 degrees C, then annealed 2 min. at room temperature, then left a few minutes on ice 0.84 μL DTT 3.36 μL water 4.2 μL dNTP mix (10 mM each) 8.4 μL 5X first strand buffer (Invitrogen) were added, then each mix was splitted into two tubes: “+RT” (were 2 μL Superscript III, Invitrogen, were added) and “-RT” (were 2 μL water were added), then the reactions were incubated 1 hour at 50 degrees C, and the enzyme was inactivated 15 min. at 70 degrees C. 2 μL of each reaction were used as templates for a PCR amplification (with 25 pmol of each primer: 5 ́- dAdAdT dGdAdT dAdCdG dGdCdG dAdCdC dAdCdC dGdAdC dAdGdG dTdTdC dAdGdA dGdTdT dCdTdA dCdAdG dTdCdC dGdA -3 ́ and 5 ́-dCdAdA dGdCdA dGdAdA dGdAdC dGdGdC dAdTdA dCdGdA dAdTdT dGdAdT dGdGdT dGdCdC dTdAdC dAdG-3 ́, and one PuReTaq Ready-To-Go PCR bead, GE Healthcare, in 25 μL): 2 min. at 94 degrees C, 5 times (15 s. at 94 degrees C, 30 s. at 54 degrees C, 30 s. at 72 degrees C), 17 times (15 s. at 94 degrees C, 30 s. at 60 degrees C, 30 s. at 72 degrees C), then 2 min. at 72 degrees C. The RT-PCR products were purified on a 2 % NuSieve GTG agarose gel, and extracted with the following protocol: the agarose bands were melted in 0.4 M NaCl (100 μL per 100 mg agarose) for 15 min. at 70 degrees C; the solution was extracted with 1 volume of phenol (buffered at pH=7.9) at room temperature, then 1 volume of phenol/chloroform/isoamylalcohol (25/24/1 v/v/v), then 1 volume chloroform/isoamylalcohol (24/1 v/v) then precipitated with 2.5 volumes ethanol. 2 pM solutions of the resulting libraries were seeded on each Solexa lane, at the Deep Sequencing Core facility of UMass Medical School (Worcester, MA, USA).

text: Nematostella vectensis

text: Generic sample from Nematostella vectensis


UEox

text: CNRS

text: live

text: Generic

text: Nematostella vectensis


BLox

text: CNRS

text: live

text: Generic

text: Nematostella vectensis


FEun

text: CNRS

text: live

text: Generic

text: Nematostella vectensis


FEox

text: CNRS

text: live

text: Generic

text: Nematostella vectensis


MAun

text: CNRS

text: live

text: Generic

text: Nematostella vectensis


MAox

text: CNRS

text: live

text: Generic

text: Nematostella vectensis


GAun

text: CNRS

text: live

text: Generic

text: Nematostella vectensis


GAox

text: CNRS

text: live

text: Generic

text: Nematostella vectensis


EPun

text: CNRS

text: live

text: Generic

text: Nematostella vectensis


EPox

text: CNRS

text: live

text: Generic

text: Nematostella vectensis


LPun

text: CNRS

text: live

text: Generic

text: Nematostella vectensis


LPox

text: CNRS

text: live

text: Generic

text: Nematostella vectensis


MEun

text: CNRS

text: live

text: Generic

text: Nematostella vectensis


MEox

text: CNRS

text: live

text: Generic

text: Nematostella vectensis


PPun

text: CNRS

text: live

text: Generic

text: Nematostella vectensis


PPox

text: CNRS

text: live

text: Generic

text: Nematostella vectensis


sessile oak LC1

text: INRA

text: live

text: Generic

text: Endodormancy, sampled in September 17th and 24th and October 1st, 2005. Number of genotypes=30. Originated from Longchamp, France.

text: Quercus petraea

text: Generic sample from Quercus petraea


sessile oak LC2

text: INRA

text: live

text: Generic

text: Ecodormancy, sampled in January 14th and 28th and February 11th, 2005. Number of genotypes=30. Originated from Longchamp, France.

text: Quercus petraea

text: Generic sample from Quercus petraea


sessile oak SJ1

text: INRA

text: live

text: Generic

text: Endodormancy, sampled in September 17th and 24th and October 1st, 2005. Number of genotypes=30. Originated from Saint Jean, France.

text: Quercus petraea

text: Generic sample from Quercus petraea


sessile oak SJ2

text: INRA

text: live

text: Generic

text: Ecodormancy, sampled in January 14th and 28th and February 11th, 2005. Number of genotypes=30. Originated from Saint Jean, France.

text: Quercus petraea

text: Generic sample from Quercus petraea


sessile oak 10QS

text: INRA

text: live

text: Generic

text: Young and mature leaves,quiescent and later buds. Number of genotypes=10.

text: Quercus petraea

text: Generic sample from Quercus petraea


pedunculate oak 10QP

text: INRA

text: live

text: Generic

text: Quercus robur

text: Generic sample from Quercus robur


sessile oak FS

text: INRA

text: live

text: Generic

text: Pollen,flowers. Number of genotypes=2

text: Quercus petraea

text: Generic sample from Quercus petraea


pedunculate oak FP

text: INRA

text: live

text: Generic

text: Pollen, flowers. Number of genotypes=2.

text: Quercus robur

text: Generic sample from Quercus robur


sessile oak Qs21

text: INRA

text: live

text: Generic

text: Quiescent,swelling buds; young,mature leaves.

text: Quercus petraea

text: Generic sample from Quercus petraea


sessile oak Qs28

text: INRA

text: live

text: Generic

text: Quercus petraea

text: Generic sample from Quercus petraea


sessile oak Qs29

text: INRA

text: live

text: Generic

text: Quercus petraea

text: Generic sample from Quercus petraea


pedunculate oak 3P

text: INRA

text: live

text: Generic

text: Quercus robur

text: Generic sample from Quercus robur


pedunculate oak 11P

text: INRA

text: live

text: Generic

text: Quercus robur

text: Generic sample from Quercus robur


pedunculate oak A04

text: INRA

text: live

text: Generic

text: Quercus robur

text: Generic sample from Quercus robur


Roseobacter sp. R2A57 Sample

text: J. Craig Venter Institute

text: live

text: Generic

text: 454 Sequencing of Rosebacter sp. R2A57

text: Roseobacter sp. R2A57

characteristic: strain

text: Generic sample from Roseobacter sp. R2A57


text: Cancer Research UK (CRUK)

text: live

text: Generic

text: LNCaP cell line, unstimulated vehicle, anti-AR N20 antibody SC-816X from Santa Cruz Biotechnologies.

text: Homo sapiens

text: Generic sample from Homo sapiens


text: Cancer Research UK (CRUK)

text: live

text: Generic

text: LNCaP cell line, AR stimulated, anti-AR N20 antibody SC-816X from Santa Cruz Biotechnologies.

text: Homo sapiens

text: Generic sample from Homo sapiens


text: Cancer Research UK (CRUK)

text: live

text: Generic

text: VCaP cell line, unstimulated vehicle, anti-AR N20 antibody SC-816X from Santa Cruz Biotechnologies.

text: Homo sapiens

text: Generic sample from Homo sapiens


text: Cancer Research UK (CRUK)

text: live

text: Generic

text: VCaP cell line, AR stimulated, anti-AR N20 antibody SC-816X from Santa Cruz Biotechnologies.

text: Homo sapiens

text: Generic sample from Homo sapiens


text: Cancer Research UK (CRUK)

text: live

text: Generic

text: LNCaP cell line, unstimulated vehicle, anti-RNAP II antibody AB-5401 form Abcam.

text: Homo sapiens

text: Generic sample from Homo sapiens


text: Cancer Research UK (CRUK)

text: live

text: Generic

text: LNCaP cell line, AR stimulated, anti-RNAP II antibody AB-5401 form Abcam.

text: Homo sapiens

text: Generic sample from Homo sapiens


text: Cancer Research UK (CRUK)

text: live

text: Generic

text: LNCaP cell line, AR stimulated, un-immunoprecipitated for use as Input.

text: Homo sapiens

text: Generic sample from Homo sapiens


text: Cancer Research UK (CRUK)

text: live

text: Generic

text: VCaP cell line, AR stimulated, un-immunoprecipitated for use as Input.

text: Homo sapiens

text: Generic sample from Homo sapiens


text: University of Georgia

text: live

text: Generic

text: Zamia vazquezii

text: Total mRNA from male cone tissue from Zamia vazquezii


text: University of Georgia

text: live

text: Generic

text: Zamia vazquezii

text: Total mRNA from female cone tissue from Zamia vazquezii


text: University of Georgia

text: live

text: Generic

text: Zamia vazquezii

text: Total mRNA from mature sporophyll tissue from Zamia vazquezii


text: University of Georgia

text: live

text: Generic

text: Zamia vazquezii

text: Total mRNA from stem tissue from Zamia vazquezii


text: University of Georgia

text: live

text: Generic

text: Zamia vazquezii

text: Total mRNA from leaflets from Zamia vazquezii


text: University of Georgia

text: live

text: Generic

text: Zamia vazquezii

text: Total mRNA from apical meristem tissue from Zamia vazquezii


text: University of Georgia

text: live

text: Generic

text: Zamia vazquezii

text: Total mRNA from seeds from Zamia vazquezii


text: University of California - Davis, Drosophila Population Genomics Project

text: live

text: Generic

text: A Drosophila melanogaster genome that is isogenic for the X chromosome (extracted with a balancer) from an isofemale line established from a single inseminated female collected in Malawi, Africa.

text: Drosophila melanogaster


text: live

text: Generic

text: A Drosophila melanogaster genome that is isogenic for chromosome 2 (extracted with a balancer) from an isofemale line established from a single inseminated female collected in Malawi, Africa.

text: Drosophila melanogaster


Worker brain bisulfite sequences

text: James Cook University

text: live

text: Generic

text: worker

text: Apis mellifera

text: brain

characteristic: tissue type

text: Apis mellifera worker brain


Queen brain bisulfite sequences

text: James Cook University

text: live

text: Generic

text: queen

text: Apis mellifera

text: brain

text: Apis mellifera queen brain


gamma proteobacterium HIMB30 Sample

text: J. Craig Venter Institute

text: live

text: Generic

text: 454 Sequencing of gamma proteobacterium HIMB30

characteristic: description

text: gamma proteobacterium HIMB30

text: Generic sample from gamma proteobacterium HIMB30


characteristic: External Id

text: Mello, Program in Molecular Medicine, University of Massachusetts Medical School

text: live

text: Generic

text: Myc monoclonal antibody (Roche, Anti-c-myc, Cat.No.11667203001) to pull down MycQDE-2 protein

characteristic: antibody

text: IP fraction

characteristic: fraction

text: wild type

characteristic: genotype/variation

text: Neurospora crassa

text: QDE-2 IP

text: QDE-2_IP Solexa sequencing


text: Mitchell A. Lazar, Institute for Diabetes, Obesity, and Metabolism, University of Pennsylvania

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: thioglycollate-elicited peritoneal macrophages

characteristic: cell type

text: PPARgamma

characteristic: chip antibody

text: Mus musculus

text: primary macrophage


characteristic: INSDC last update

characteristic: INSDC secondary accession

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: cell line

text: PPARgamma

text: Mus musculus


text: live

text: Generic

characteristic: NCBI submission package

characteristic: SRA accession

text: Mus musculus

characteristic: organism

text: primary macrophage

characteristic: title


characteristic: External Id

text: live

text: Generic

text: C/EBPbeta

text: Mus musculus

text: primary macrophage

characteristic: strain


text: live

text: Generic

text: Mus musculus


text: State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology

text: live

text: Generic

characteristic: ecotype

text: wild-type

text: Arabidopsis thaliana

text: wild-type

text: seedlings

characteristic: tissue


characteristic: INSDC center name

text: live

text: Generic

text: atprmt5 mutant

characteristic: genotype/variation

text: Arabidopsis thaliana

text: atprmt5 mutant

text: seedlings


text: Greenberg, Neurobiology, Harvard Medical School

characteristic: INSDC first public

text: live

text: Generic

text: untreated

characteristic: agent

text: MAB1133 (AC238) Millipore

characteristic: antibody

characteristic: biological rep

text: cortical neuron

characteristic: cell type

text: DIV 7, E16.5

characteristic: developmental stage

text: Mus musculus

text: CBP

characteristic: protein immunoprecipitated

text: 7 DIV E16.5 mouse cortical neurons, before and after membrane depolarization induced by extracellular potassium

characteristic: technical rep


characteristic: INSDC last update

characteristic: INSDC secondary accession

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

characteristic: SRA accession

text: KCL

text: MAB1133 (AC238) Millipore

text: cortical neuron

text: DIV 7, E16.5

text: Mus musculus

text: CBP


characteristic: External Id

text: live

text: Generic

text: untreated

text: Ab2832, Abcam

text: cortical neuron

text: DIV 7, E16.5

text: Mus musculus

characteristic: organism

text: CBP

characteristic: strain

characteristic: title


characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: KCL

characteristic: agent

text: Ab2832, Abcam

text: cortical neuron

text: DIV 7, E16.5

text: Mus musculus

text: CBP


text: live

text: Generic

text: untreated

text: 4H8, Abcam (Ab5408)

characteristic: antibody

characteristic: biological rep

text: cortical neuron

characteristic: cell type

text: DIV 7, E16.5

text: Mus musculus

text: RNAPII


text: live

text: Generic

text: KCL

text: 4H8, Abcam (Ab5408)

text: cortical neuron

text: DIV 7, E16.5

characteristic: developmental stage

text: Mus musculus

text: RNAPII

characteristic: protein immunoprecipitated

characteristic: technical rep


characteristic: INSDC last update

characteristic: INSDC secondary accession

text: live

characteristic: INSDC status

text: Generic

text: untreated

text: 8WG16, Covance (MMS-126R)

text: cortical neuron

text: DIV 7, E16.5

text: Mus musculus

text: RNAPII


text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

characteristic: SRA accession

text: KCL

text: 8WG16, Covance (MMS-126R)

text: cortical neuron

text: DIV 7, E16.5

text: Mus musculus

text: RNAPII


characteristic: External Id

text: live

text: Generic

text: untreated

text: custom

text: cortical neuron

text: DIV 7, E16.5

text: Mus musculus

characteristic: organism

characteristic: strain


characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: KCL

text: custom

text: cortical neuron

text: DIV 7, E16.5

text: Mus musculus

characteristic: title


text: live

text: Generic

text: untreated

characteristic: agent

text: Santa Cruz (Sc-186)

characteristic: antibody

characteristic: biological rep

text: cortical neuron

text: DIV 7, E16.5

text: Mus musculus


text: live

text: Generic

text: KCL

text: Santa Cruz (Sc-186)

text: cortical neuron

characteristic: cell type

text: DIV 7, E16.5

characteristic: developmental stage

text: Mus musculus

characteristic: protein immunoprecipitated


characteristic: INSDC last update

characteristic: INSDC secondary accession

text: live

text: Generic

text: untreated

text: H300 (Sc-13029)

text: cortical neuron

text: DIV 7, E16.5

text: Mus musculus

text: SRF

characteristic: technical rep


text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: KCL

text: H300 (Sc-13029)

text: cortical neuron

text: DIV 7, E16.5

text: Mus musculus

text: SRF


characteristic: External Id

text: live

text: Generic

characteristic: SRA accession

text: untreated

text: Abcam (Ab1012)

text: cortical neuron

text: DIV 7, E16.5

text: Mus musculus

characteristic: organism


characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: KCL

text: Abcam (Ab1012)

text: cortical neuron

text: DIV 7, E16.5

text: Mus musculus

characteristic: strain


text: live

text: Generic

text: untreated

characteristic: agent

text: Millipore (07-449)

characteristic: antibody

text: cortical neuron

text: DIV 7, E16.5

text: Mus musculus

characteristic: title


text: live

text: Generic

text: KCL

text: Millipore (07-449)

characteristic: biological rep

text: cortical neuron

characteristic: cell type

text: DIV 7, E16.5

characteristic: developmental stage

text: Mus musculus


characteristic: INSDC last update

characteristic: INSDC secondary accession

text: live

text: Generic

text: untreated

text: Abcam (Ab8895)

text: cortical neuron

text: DIV 7, E16.5

text: Mus musculus

characteristic: protein immunoprecipitated


text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: KCL

text: Abcam (Ab8895)

text: cortical neuron

text: DIV 7, E16.5

text: Mus musculus

characteristic: technical rep


characteristic: External Id

text: live

text: Generic

characteristic: NCBI submission package

characteristic: SRA accession

text: KCL

text: cortical neuron

text: DIV 7, E16.5

text: Mus musculus


characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: untreated

text: cortical neuron

text: DIV 7, E16.5

text: Mus musculus

characteristic: organism


text: live

text: Generic

text: untreated

characteristic: agent

text: cortical neuron

text: DIV 7, E16.5

text: Mus musculus

characteristic: strain

text: 1 and 2


text: live

text: Generic

text: KCL

characteristic: biological rep

text: cortical neuron

text: DIV 7, E16.5

text: Mus musculus

text: 1 and 2

characteristic: title


text: live

text: Generic

text: untreated

text: cortical neuron

characteristic: cell type

text: DIV 7, E16.5

characteristic: developmental stage

text: Mus musculus


characteristic: INSDC last update

characteristic: INSDC secondary accession

text: live

text: Generic

text: KCL

text: cortical neuron

text: DIV 7, E16.5

text: Mus musculus


text: HudsonAlpha

text: live

characteristic: INSDC status

text: Generic

text: ChIP-seq was used to find the binding sites for the transcription factor Sin3a in hESC cells as described in the Study category for this entry. The replicate number indicates replicate 1. For more details please contact the Myers Lab at the HudsonAlpha Institute for Biotechnology.

characteristic: description

text: Homo sapiens


text: HudsonAlpha

text: live

text: Generic

characteristic: NCBI submission model

text: ChIP-seq was used to find the binding sites for the transcription factor Sin3a in hESC cells as described in the Study category for this entry. The replicate number indicates rep2. For more details please contact the Myers Lab at the HudsonAlpha Institute for Biotechnology.

text: Homo sapiens


characteristic: External Id

text: NCBI-Whole Genome Shotgun Submissions

text: live

text: Generic

text: Indonesia: Mount Lawu, Central Java

characteristic: country

text: The unicellular red micro-alga Galdieria sulphuraria (Cyanidiales) is a eukaryote that can represent up to 90% of the biomass in extreme habitats such as hot sulfur springs with pH values of 0 to 4 and temperatures of up to 56*C. This red alga thrives autotropically as well as heterotropically on more that 50 different carbon sources, including a number of rare sugars and sugar alcohols.

text: Galdieria sulphuraria

text: Galdieria sulphuraria strain 074W


text: HudsonAlpha

text: live

text: Generic

characteristic: NCBI submission package

characteristic: SRA accession

text: ChIP-seq was used to find the binding sites for the transcription factor Six5 in hESC cells as described in the Study category for this entry. The replicate number indicates rep1. For more details please contact the Myers Lab at the HudsonAlpha Institute for Biotechnology.

text: Homo sapiens


text: HudsonAlpha

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: ChIP-seq was used to find the binding sites for the transcription factor Six5 in hESC cells as described in the Study category for this entry. The replicate number indicates rep2. For more details please contact the Myers Lab at the HudsonAlpha Institute for Biotechnology.

text: Homo sapiens


text: HudsonAlpha

text: live

text: Generic

text: ChIP-seq was used to find the binding sites for the transcription factor SP1 in hESC cells as described in the Study category for this entry. The replicate number indicates rep1. For more details please contact the Myers Lab at the HudsonAlpha Institute for Biotechnology.

text: Homo sapiens

characteristic: organism


text: HudsonAlpha

text: live

text: Generic

text: ChIP-seq was used to find the binding sites for the transcription factor SP1 in hESC cells as described in the Study category for this entry. The replicate number indicates rep2. For more details please contact the Myers Lab at the HudsonAlpha Institute for Biotechnology.

text: Homo sapiens


text: HudsonAlpha

text: live

text: Generic

text: ChIP-seq was used to find the binding sites for the transcription factor HEB in hESC cells as described in the Study category for this entry. The replicate number indicatesFor more details please contact the Myers Lab at the HudsonAlpha Institute for Biotechnology.

text: Homo sapiens


text: HudsonAlpha

text: live

text: Generic

text: ChIP-seq was used to find the binding sites for the transcription factor HEB in hESC cells as described in the Study category for this entry. The replicate number indicates rep2. For more details please contact the Myers Lab at the HudsonAlpha Institute for Biotechnology.

text: Homo sapiens


text: HudsonAlpha

text: live

text: Generic

text: ChIP-seq was used to find the binding sites for the transcription factor USF1 in hESC cells as described in the Study category for this entry. The replicate number indicates rep1. For more details please contact the Myers Lab at the HudsonAlpha Institute for Biotechnology.

text: Homo sapiens


text: HudsonAlpha

text: live

text: Generic

text: ChIP-seq was used to find the binding sites for the transcription factor USF1 in hESC cells as described in the Study category for this entry. The replicate number indicates rep2. For more details please contact the Myers Lab at the HudsonAlpha Institute for Biotechnology.

text: Homo sapiens


text: Cornell University

text: live

text: Generic

text: Anliu sweet orange, wildtype

characteristic: genotype

text: Citrus sinensis

text: fruits

text: fruits, 170 DAF (days after flowering)

characteristic: tissue


text: Cornell University

text: live

text: Generic

text: red-flesh mutant

text: Citrus sinensis

text: fruits

text: fruits, 170 DAF (days after flowering)

characteristic: title


text: roelink, MCB, UC Berkeley

text: live

text: Generic

text: control

characteristic: agent

characteristic: cell type

text: Mus musculus

text: C3H10T1/2, control treated


text: roelink, MCB, UC Berkeley

characteristic: INSDC last update

text: live

text: Generic

text: control

text: MIA PaCa-2

text: Homo sapiens

text: MIA PaCa-2, control treated


text: roelink, MCB, UC Berkeley

characteristic: INSDC secondary accession

text: live

text: Generic

text: control

text: C3H10T1/2 / MIA PaCa-2 coculture

text: Homo sapiens

text: C3H10T1/2 / MIA PaCa-2 coculture, control treated


text: roelink, MCB, UC Berkeley

text: live

characteristic: INSDC status

text: Generic

text: 5E1 blocking antibody

text: C3H10T1/2 / MIA PaCa-2 coculture

text: Homo sapiens

text: C3H10T1/2 / MIA PaCa-2 coculture, 5E1 treated


text: University of East Anglia

text: live

text: Generic

text: Vitis vinifera

text: Vitis vinifera cv. Pinot noir, clone ENTAV115, leaves and stem

text: RNA extracted from stem and leaves

text: sRNA from grape stem and leaves


text: University of East Anglia

text: live

text: Generic

text: Vitis vinifera

text: Vitis vinifera cv. Pinot noir, clone ENTAV115, tendrils

text: RNA extracted from tendrils

text: sRNA from grape tendrils


text: University of East Anglia

text: live

text: Generic

text: Vitis vinifera

text: Vitis vinifera cv. Pinot noir, clone ENTAV115, inflorescences

text: RNA extracted from inflorescences

text: sRNA from grape inflorescences


text: University of East Anglia

text: live

text: Generic

text: Vitis vinifera

text: Vitis vinifera cv. Pinot noir, clone ENTAV115, small berries and bunch stems

text: RNA extracted from small berries and bunch stems

text: sRNA from grape berries


text: University of East Anglia

text: live

text: Generic

text: Vitis vinifera

text: RNA extracted from stem and leaves

text: Vitis vinifera leaf parallel analysis of RNA ends


text: Genome Science Division, RCAST, The University of Tokyo

text: live

text: Generic

characteristic: antibody

text: Mouse embryonic fibroblast

text: Mus musculus

characteristic: passage

text: Mouse embryonic fibroblast


characteristic: External Id

text: live

text: Generic

characteristic: NCBI submission model

text: Mouse embryonic fibroblast

text: Mus musculus

text: Mouse embryonic fibroblast


text: live

text: Generic

characteristic: NCBI submission package

text: Mouse embryonic fibroblast

text: Mus musculus

text: Mouse embryonic fibroblast

text: infected with activated RasV12-expressing retrovirus

characteristic: treatment


characteristic: INSDC center name

text: live

text: Generic

characteristic: SRA accession

text: Mouse embryonic fibroblast

text: Mus musculus

text: Mouse embryonic fibroblast


text: University of East Anglia

characteristic: INSDC first public

text: live

text: Generic

text: Micro-Tom

characteristic: cultivar

text: closed bud before flower blooming

characteristic: developmental stage

text: Solanum lycopersicum

characteristic: organism

text: Closed bud before flower blooming

text: closed bud

text: sRNA from closed bud before flower blooming


text: University of East Anglia

text: live

text: Generic

text: Micro-Tom

text: open flower

text: Solanum lycopersicum

text: Open flower

text: flower

characteristic: tissue

text: sRNA from open flower

characteristic: title


text: University of East Anglia

characteristic: INSDC last update

text: live

text: Generic

text: Micro-Tom

text: 1-3mm diameter fruit

text: Solanum lycopersicum

text: 1-3mm fruit

text: fruit

text: sRNA from tomato fruit 1mm to 3mm in diameter


text: University of East Anglia

characteristic: INSDC secondary accession

text: live

text: Generic

text: Micro-Tom

text: 5-7mm diameter fruit

text: Solanum lycopersicum

text: 5-7mm fruit

text: fruit

text: sRNA from tomato fruit 5mm to 7mm in diameter


text: University of East Anglia

text: live

characteristic: INSDC status

text: Generic

text: Micro-Tom

text: 11-14mm diameter fruit

text: Solanum lycopersicum

text: 11-14mm fruit

text: fruit

text: sRNA from tomato fruit 11mm to 14mm in diameter


text: University of East Anglia

text: live

text: Generic

text: Micro-Tom

text: mature green fruit

text: Solanum lycopersicum

text: mature green fruit

text: fruit

text: sRNA from tomato mature green fruit


text: University of East Anglia

text: live

text: Generic

text: Micro-Tom

text: breaker stage fruit

text: Solanum lycopersicum

text: breaker stage fruit

text: fruit

text: sRNA from tomato breaker stage fruit


text: University of East Anglia

text: live

text: Generic

text: Micro-Tom

text: fruit 3 days post breaker stage

text: Solanum lycopersicum

text: breaker +3 days fruit

text: fruit

text: sRNA from tomato fruit 3 days post breaker


text: University of East Anglia

text: live

text: Generic

text: Micro-Tom

text: fruit 5 days post breaker stage

text: Solanum lycopersicum

text: breaker +5 days fruit

text: fruit

text: sRNA from tomato fruit 5 days post breaker


text: University of East Anglia

text: live

text: Generic

text: Micro-Tom

text: fruit 7 days post breaker stage

text: Solanum lycopersicum

text: breaker +7 days fruit

text: fruit

text: sRNA from tomato fruit 7 days post breaker


text: Developmental Genomics, LCDB, NIDDK, NIH

text: live

text: Generic

characteristic: cell line

text: ERCC phase V pool 15

characteristic: external rna control

text: Drosophila melanogaster

text: untreated

characteristic: s2 treatment

text: Drosophila S2 cell and 5ng ERCC RNA controls

text: 100ng_S2_5ng_ERCC_phaseV_pool15_mRNA-seq


characteristic: External Id

text: live

text: Generic

characteristic: NCBI submission model

text: ERCC phase V pool 15

text: Drosophila melanogaster

text: msl2 RNAi

text: Drosophila S2 cell and 2.5ng ERCC RNA controls

text: 100ng_S2_2.5ng_ERCC_phaseV_pool15_mRNA-seq


text: live

text: Generic

characteristic: NCBI submission package

text: ERCC phase V pool 15

text: Drosophila melanogaster

text: mof RNAi

text: Drosophila S2 cell and 1ng ERCC RNA controls


characteristic: INSDC center name

text: live

text: Generic

text: ERCC phase V pool 15

text: synthetic construct

text: ERCC RNA controls only


text: sjtu

text: live

text: Generic

characteristic: SRA accession

text: V3 region of 16S rRNA in mouse fecal sample

characteristic: description

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

characteristic: INSDC first public

text: live

text: Generic

text: V3 region of 17S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 18S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 19S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 20S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 21S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 22S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 23S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 24S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 25S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 26S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 27S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 28S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 29S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 30S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 31S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 32S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 33S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 34S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 35S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 36S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 37S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 38S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 39S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 40S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 41S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 42S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 43S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 44S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 45S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 46S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 47S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 48S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 49S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 50S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 51S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 52S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 53S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 54S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 55S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 56S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 57S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 58S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 59S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 60S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 61S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 62S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 63S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 64S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 65S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 66S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 67S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 68S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 69S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 70S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 71S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 72S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 73S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 74S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 75S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 76S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 77S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 78S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 79S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 80S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 81S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 82S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 83S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 84S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 85S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 86S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 87S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 88S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 89S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 90S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 91S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 92S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 93S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 94S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 95S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 96S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 97S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 98S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 99S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 100S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 101S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 102S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 103S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 104S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 105S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 106S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 107S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 108S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 109S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 110S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 111S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 112S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 113S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 114S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 115S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 116S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 117S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 118S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 119S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 120S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 121S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 122S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 123S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 124S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 125S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 126S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 127S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 128S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 129S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 130S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 131S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 132S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 133S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 134S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 135S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 136S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 137S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 138S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 139S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 140S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 141S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 142S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 143S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 144S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 145S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 146S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 147S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 148S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 149S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 150S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 151S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 152S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 153S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 154S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 155S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 156S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 157S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 158S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 159S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 160S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 161S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 162S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 163S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 164S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 165S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 166S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 167S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 168S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 169S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 170S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 171S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 172S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 173S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 174S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 175S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 176S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 177S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 178S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 179S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 180S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 181S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 182S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 183S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 184S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 185S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 186S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 187S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 188S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 189S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 190S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 191S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 192S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 193S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 194S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 195S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 196S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 197S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 198S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 199S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 200S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 201S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 202S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 203S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 204S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 205S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 206S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 207S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 208S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 209S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 210S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 211S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 212S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 213S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 214S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 215S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 216S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 217S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 218S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 219S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 220S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 221S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 222S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 223S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 224S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 225S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 226S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 227S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 228S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 229S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 230S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 231S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 232S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 233S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 234S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 235S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 236S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 237S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 238S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 239S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 240S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 241S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 242S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 243S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 244S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 245S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 246S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 247S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 248S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 249S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 250S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 251S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 252S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 253S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 254S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 255S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 256S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 257S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 258S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 259S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 260S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 261S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 262S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 263S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 264S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 265S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 266S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 267S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 268S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 269S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 270S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 271S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 272S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 273S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 274S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 275S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 276S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 277S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 278S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 279S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 280S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 281S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 282S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 283S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 284S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 285S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 286S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 287S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 288S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 289S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 290S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 291S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 292S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 293S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 294S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 295S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 296S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 297S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 298S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 299S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 300S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 301S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 302S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: V3 region of 303S rRNA in mouse fecal sample

text: mouse gut metagenome

text: mouse gut metagenome


text: sjtu

text: live

text: Generic

text: Mouse gut metagenome, default sample for unclassified reads

text: mouse gut metagenome

text: mouse gut metagenome


text: Hubrecht Institute

text: live

text: Generic

characteristic: antibody

text: Caenorhabditis elegans

text: small RNA from HA::ALG-1 IP

characteristic: strain


text: Hubrecht Institute

text: live

text: Generic

text: Caenorhabditis elegans

characteristic: organism

text: small RNA from a HA::ALG-2 IP


text: Hubrecht Institute

text: live

text: Generic

text: Caenorhabditis elegans

text: small RNA from a HA::RDE-1 IP

characteristic: title


text: Hubrecht Institute

characteristic: INSDC last update

text: live

text: Generic

text: Caenorhabditis elegans

text: small RNA from rde-1 mutants (allele pk3301)


text: Hubrecht Institute

characteristic: INSDC secondary accession

text: live

text: Generic

text: Caenorhabditis elegans

text: small RNA from N2 wild-type adults


T.selenatis

text: University of Exeter

text: live

characteristic: INSDC status

text: Generic

text: Thauera selenatis is a member of the -subclass of proteobacteria and possesses a mechanism for anaerobic respiration that can utilise selenate as the sole electron acceptor. Here we report the completion of the genome sequence of T. selenatis ATCC 55363. The genes encoding respiratory pathways have been found in the genome along with operons for metal resistance and protein export systems. The T. selenatis genome will allow us to make considerable progress in our understanding of the mechanism of dissimilatory reduction of selenium oxyanions, which should prove to be valuable when designing better systems for the bio-remediation of toxic seleniferous wastewaters.

text: Thauera selenatis

text: T.selenatis genome


MB

text: The Chinese University of Hong Kong

text: live

text: Generic

characteristic: collection date

text: Surface seawater samples (~2.5 l) were collected from two bays in northeastern Hong Kong in April 2007. Tolo Harbor (TH) is a landlocked bay with a long history of eutrophication (Wear et al., 1984; Chau, 2007). Located outside TH, Mirs Bay (MB) is relatively unpolluted and more exposed to ocean currents from the South China Sea (Hong Kong Environmental Protection Department, 2003). Water samples were filtered through a 200 μm mesh sieve immediately to remove most of the mesozooplankton and large particles.

text: 22.512250 N 114.359100 E

characteristic: lat lon

text: marine metagenome

text: environmental samples from Mirs Bay


TH

text: The Chinese University of Hong Kong

text: live

text: Generic

text: 22.433585 N 114.224067 E

text: marine metagenome

text: environmental samples from Tolo Harbor


BGI-DUCK

text: BGI

text: live

text: Generic

text: Pekin duck

characteristic: breed

text: China: Beijing

characteristic: country

text: Gold Star Duck Production

characteristic: isolation-source

text: Anas platyrhynchos

text: female

characteristic: sex

text: A female beijing duck


MedDCM-OCT

characteristic: External Id

text: Universidad Miguel Hernandez - Evolutionary Genomics Group

characteristic: INSDC center name

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Seawater was collected from the DCM layer (50 m deep) off the coast of Alicante, Spain with a Niskin bottle on October 15, 2007. Total DNA was isolated from the pico-planktonic 5-0.2 micrometer size fraction. A fosmid library was created from this DNA (ca. 13000 fosmids) and a part of the DNA from this sample was also sequenced using directly pyrosequencing.

text: marine metagenome

text: metagenome from a single sample of a mature Mediterranean DCM community


text: Baylor College of Medicine

characteristic: INSDC first public

text: live

text: Generic

characteristic: SRA accession

text: CS flies were reared at room temperature (23-24C) for collection. Whole body samples were homogenized in 1ml of Trizol (Invitrogen, Carlsbad, California, USA) per 50-100 mg of tissue using a glass-Teflon homogenizer. RNA was purified according to the suggested manufacturer's protocol (Invitrogen). Eggs were laid on grape juice plates for 2 hours and then removed and aged for 2 more hours.

characteristic: description

text: Drosophila melanogaster

text: Embryo 2-4 hours RNA-Seq


text: Baylor College of Medicine

text: live

text: Generic

text: CS flies were reared at room temperature (23-24C) for collection. Whole body samples were homogenized in 1ml of Trizol (Invitrogen, Carlsbad, California, USA) per 50-100 mg of tissue using a glass-Teflon homogenizer. RNA was purified according to the suggested manufacturer's protocol (Invitrogen). Eggs were laid on grape juice plates for 2 hours and then removed and aged for 14 more hours.

text: Drosophila melanogaster

characteristic: organism

text: Embryo 14-16 hours RNA-Seq

characteristic: title


text: Baylor College of Medicine

characteristic: INSDC last update

characteristic: INSDC secondary accession

text: live

text: Generic

text: CS flies were reared at room temperature (23-24C) for collection. Whole body samples were homogenized in 1ml of Trizol (Invitrogen, Carlsbad, California, USA) per 50-100 mg of tissue using a glass-Teflon homogenizer. RNA was purified according to the suggested manufacturer’s protocol (Invitrogen). Eggs were laid on grape juice plates for 14 hours and then removed and aged for 2 more hours.

text: Drosophila melanogaster

text: Embryo 2-16 hours RNA-Seq


name: Eucalyptus grandis x Eucalyptus urophylla gene catalog

text: University of Pretoria

text: live

characteristic: INSDC status

text: Generic

text: De novo assembled expressed gene catalog of a fast-growing Eucalyptus plantation tree produced by Illumina mRNA-Seq


text: The Hamner Institutes for Health Sciences

text: live

text: Generic

text: Forty five day-old female C57BL/6 mice were obtained from Charles River Laboratories (Raleigh, NC) and acclimated for 2 weeks. The control mice were given distilled deionized water only. At the end of the study, mice were killed by exsanguination under CO2 anesthesia. The lung tissue was harvested immediately, placed in RNAlater™ (Ambion, Austin, TX), and stored at −70 °C. A portion of frozen lung tissue was removed from each sample and used to isolate genomic DNA. Tissue from each treatment group was suspended in 1.2 ml cell lysis buffer (10 mM NaCl, 10 mM TrisCl pH 7.8, 2 mM EDTA) on ice and homogenized for 2 min (sterile 3 mm steel ball, Mixer Mill 301, Retsch, Newtown, PA). After addition of 125 μl 10% SDS solution and 23 μl Proteinase K (20 mg/ml) (Invitrogen, Carlsbad, CA) the homogenate was incubated at 55 °C for 3 h which was followed by the addition of 13 μl DNAse-free RNAse A (2 mg/ml) (Sigma-Aldrich, St. Louis, MO) and an incubation for 30 min at 37 °C. Protein precipitation was carried out by mixing 1340 μl tissue lysate 1:1 with Phenol:Chloroform:Isoamylalcohol (Invitrogen, Carlsbad, CA) in Phase Lock Gel Heavy Tubes (5Prime, Gaithersburg, MD) followed by 15 min centrifugation at 3200xg. 1:1 Chloroform extraction of the aqueous phase at 3200xg was followed by the addition of 1/40 volume 3 M sodium acetate solution and 2 volumes ethanol. Genomic DNA was precipitated at 16000xg and washed with 70% ethanol before resuspension of the air dried pellet in 10 mM Tris 1mM EDTA pH 7.5. Following resuspension, equal amounts of genomic DNA from the five arsenate-treated animals were pooled together and equal amounts of genomic DNA from the five control animals were pooled together. Five μg of pooled genomic DNA was digested with 400 U MspI (New England Biolabs, Ipswich, MA) in 100 μl at 37 °C for 18 - 24 hrs followed by QIAQuick purification (Qiagen, Valencia, CA). The end of digested DNA was filled in and an adenine was added to the 3’ end of the DNA fragments according to the Illumina standard end repair and add_A protocol (Illumina, San Diego, CA). Pre-annealed forked Illumina adaptors containing 5’-methyl-cytosine instead of cytosine was ligated to both ends of DNA fragments using standard Illumina adaptor ligation protocol (Illumina). Ligated fragments were then separated by 2% agarose gel (Sigma-Aldrich). Two size ranges, 150-175 bp and 175-225 bp (includes adaptor length), were selected and cut from the gel. DNA from gel slices were purified using Qiagen Gel extraction kit (Qiagen). The purified DNA was treated with EpiTect Bisulfite kit (Qiagen) with modification. The bisulfite conversion time was extended to approximately 14 hrs by adding 3 cycles of denaturation at 95 °C for 5 min followed by incubation at 60 °C for 180 min. The bisulfite-converted DNA was purified using the EpiTect Bisulfite kit and protocol for DNA isolated from formaldehyde-fixed, paraffin-embedded tissue samples with modification. The desulfonation buffer was added to the spin column and incubated for 20 min instead of 15 min at room temperature. The bisulfite-treated DNA was purified a second time with MinElute PCR purification kit (Qiagen) and eluted with 15 μl EB buffer. The bisulfite-treated DNA fragments were amplified using polymerase chain reaction (PCR) and the following reaction: 15 μl of eluted DNA, 5 pmol of Illumina PE PCR primers 1.0 and 2.0, 62.5 nM of each dNTP, and 2.5 U of Pfu Turbo Cx hotstart DNA polymerase (Stratagene Products, Agilent, La Jolla, CA) in a total 50 μl volume. The amplification conditions were as follows: 5 min at 95 °C, 30 sec at 98 °C then 6X (10 sec at 98 °C, 30 sec at 65 °C, 30 sec at 72 °C) followed by 5 min at 72 °C. The PCR reaction was purified by MinElute PCR purification Kit (Qiagen) and final reduced representation bisulfite library was eluted in 15 μl EB. The concentration of final library was measured using the Agilent 2100 Bioanalyzer (Palo Alto, CA). The library was sequenced on Illumina GA sequencing instrument according to standard Illumina cluster generation and sequencing protocols.

text: Mus musculus

text: Control mice not exposed to arsenate