text: The Samuel Roberts Noble Foundation

text: live

text: Generic

text: Extra-radical mycelium from Glomus intraradices grown in a two-compartment petri-plate system in association with Daucus carota transformed roots. M medium containing 35 micromolar potassium phosphate was added to the extra-radical mycelium 12 hours prior to harvest. cDNA was prepared from total RNA using the Clontech Smart cDNA synthesis system. The cDNA was directionally ligated into Lambda TriplEX2 vector (Clontech) and packaged using Gigapack Gold packaging extracts. Plasmids (pTriplEX2) containing cDNA inserts were obtained from the recombinant lambda TriplEX2 phage via Cre-lox mediated conversion in BM25.8 cells (Clontech).

text: Rhizophagus intraradices

text: Extra-radical mycelium

text: Glomus intraradices extra-radical mycelium

text: plasmid

text: Lambda TriplEX2


status: PUBLIC

characteristic: External Id

text: Harvard University, Howard Hughes Medical Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Constructed with lambda ZAPII system (Stratagene) by Dr. J. Ferrer, in vivo mass-excised to pBluescript SK- by Dr. H. Inoue following the Washington University protocol (http://genome.wustl.edu/est/lambda_protocol.shtml). Please contact Hiroshi Inoue, MD/PhD for further information on this library (Metabolism Division, Permutt Laboratory, Washington University School of Medicine, Box 8127, 660 S Euclid Ave, St. Louis, MO 63110). Note: this is a Washington University Pancreas EST project library.

characteristic: description

text: DH10B (phage-resistant)

tag: attribute

characteristic: lab-host

text: pancreas

characteristic: organ

text: Homo sapiens

characteristic: organism

text: XhoI

text: EcoRI

text: insulinoma

characteristic: tissue-type

text: Human insulinoma

characteristic: title

text: phagemid (ampicillin resistant)

text: pBluescript SK-

characteristic: vector


text: US Department of Agriculture, Agriculture Research Service, Pacific West Area, Western Regional Research Center

text: live

text: Generic

text: Cheyenne

characteristic: cultivar

text: Seeds collected, endosperm isolated, and RNA prepared by Susan Altenbach. Library constructed by Stratagene, Inc. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab. The cDNA clones were in vivo excised to give pBluescript phagemids before normalization was carried out. The mass excision of phagemid library and normalization were done in HT Nguyen lab by D. Zhang at Texas Tech University. Normalization protocol used was that of Soares' with modification.

text: 5 -30 days post anthesis seed

characteristic: dev-stage

text: E. coli SOLR

text: Triticum aestivum

text: EcoRI

text: Endosperm

text: Wheat endosperm normalized cDNA library

text: Phagemid

text: Lambda ZAP II, excised phagemid


text: live

text: Generic

text: Plants were grown under hydroponic conditions, root tips were excised and snap frozen, total RNA was prepared at University of Missouri(Ross, Gustafson). Poly(A) RNA was purified, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript SK- phagemids in the TJ Close lab (Chin and Close) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

text: Seedling

text: E. coli SOLR

text: Triticum aestivum

text: EcoRI

text: XhoI

text: Root tip at 1.0 to 1.5 mm stage

text: Wheat aluminum-stressed root tip cDNA library

text: Phagemid pBluescript SK-

text: Lambda Uni-ZAP XR, excised phagemid


text: New Mexico State University

text: live

text: Generic

text: Early Scotch Bonnet

text: drought-stressed

text: leaf

text: Capsicum chinense

text: Xho 1

text: Habanero leaf cDNA

text: Phage

text: Uni-zap XR


text: Kellogg Eye Center, University of Michigan

text: live

text: Generic

text: juvenile

text: Retina

text: Homo sapiens

text: Native Retinal Pigment Epithelium sheets

text: Human Retinal Pigment Epithelium (2)

text: Plasmid


text: live

text: Generic

text: RNA source anonymous pool of 6 male brains, age range 23-27 yo. Library is oligo-dT primed and directionally cloned (EcoRV site is destroyed upon cloning). Average insert size 1.5 kb, insert size range 1-3 kb. Library is normalized and enriched for full-length clones and was constructed by C. Gruber (Invitrogen). Research Genetics tracking code 019. Note: this is a NIH_MGC Library.

text: brain

text: Homo sapiens

text: NotI

text: EcoRV (destroyed)

text: plasmid (ampicillin resistant)


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: RNA source anonymous pool of 6 male brains, age range 23-27; 1 male lung, age 27; and 1 male testis, age 69. Library is oligo-dT primed and directionally cloned (EcoRV site is destroyed upon cloning). Average insert size 1.8 kb, insert size range 1-3 kb. Library is normalized and enriched for full-length clones and was constructed by C. Gruber (Invitrogen). Research Genetics tracking code 021. Note: this is a NIH_MGC Library.

text: pooled brain, lung, testis

text: Homo sapiens

text: NotI

text: EcoRV (destroyed)

text: plasmid (ampicillin resistant)


text: live

text: Generic

text: RNA source anonymous pool of 3 colons, age 26 yo male, 49 yo female, 71 yo male colon; 46 yo male kidney, and pool of 2 stomachs, 62 yo male and 70 yo female. Library is oligo-dT primed and directionally cloned (EcoRV site is destroyed upon cloning). Average insert size 1.4 kb, insert size range 1-3 kb. Library is normalized and enriched for full-length clones and was constructed by C. Gruber (Invitrogen). Research Genetics tracking code 023. Note: this is a NIH_MGC Library.

text: pooled colon, kidney, stomach

text: Homo sapiens

text: NotI

text: EcoRV (destroyed)

text: plasmid (ampicillin resistant)


text: live

text: Generic

text: RNA source leukocytes from anonymous pool of non-activated adult donors. Library is oligo-dT primed and directionally cloned (EcoRV site is destroyed upon cloning). Average insert size 1.7 kb, insert size range 1.2-3.3 kb. Library is normalized and enriched for full-length clones and was constructed by C. Gruber (Invitrogen). Research Genetics tracking code 027. Note: this is a NIH_MGC Library.

text: Homo sapiens

text: NotI

text: EcoRV (destroyed)

text: leukocyte

text: plasmid (ampicillin resistant)


text: live

text: Generic

text: RNA source normal medulla from anonymous male age 27. Library is oligo-dT primed and directionally cloned (EcoRV site is destroyed upon cloning). Average insert size 1.3 kb, insert size range 0.9-3 kb. Library is normalized and enriched for full-length clones and was constructed by C. Gruber (Invitrogen). Research Genetics tracking code 013. Note: this is a NIH_MGC Library.

text: brain

text: Homo sapiens

text: NotI

text: EcoRV (destroyed)

text: medulla

text: plasmid (ampicillin resistant)


text: live

text: Generic

text: RNA source anonymous pool of spleen and pancreas from 28 yo male. Library is oligo-dT primed and directionally cloned (EcoRV site is destroyed upon cloning). Average insert size 1.5 kb, insert size range 1-2.5 kb. Library is normalized and enriched for full-length clones and was constructed by C. Gruber (Invitrogen). Research Genetics tracking code 025. Note: this is a NIH_MGC Library.

text: pooled pancreas and spleen

text: Homo sapiens

text: NotI

text: EcoRV (destroyed)

text: plasmid (ampicillin resistant)


text: live

text: Generic

text: RNA source anonymous pool of 3 fetal brains, female age 20 weeks, female age 24 weeks, and male age 26 weeks. Library is oligo-dT primed and directionally cloned (EcoRV site is destroyed upon cloning). Average insert size 1.7 kb, insert size range 0.7-3.5 kb. Library is normalized and enriched for full-length clones and was constructed by C. Gruber (Invitrogen). Research Genetics tracking code 017. Note: this is a NIH_MGC Library.

text: brain

text: Homo sapiens

text: NotI

text: EcoRV (destroyed)

text: plasmid (ampicillin resistant)


text: live

text: Generic

text: RNA source anonymous pool of 24 week female lung, 16 week female spleen, and 20-22 week male spleens. Library is oligo-dT primed and directionally cloned (EcoRV site is destroyed upon cloning). Average insert size 1.4 kb, insert size range 1-3 kb. Library is normalized and enriched for full-length clones and was constructed by C. Gruber (Invitrogen). Research Genetics tracking code 026. Note: this is a NIH_MGC Library.

text: pooled lung and spleen

text: Homo sapiens

text: NotI

text: EcoRV (destroyed)

text: plasmid (ampicillin resistant)


text: Shanghai Institute of Cell Biology

text: live

text: Generic

text: Homo sapiens

text: Homo sapiens breast cancer-related


text: Shanghai Institute of Endocrinology

text: live

text: Generic

text: heart

text: Rattus norvegicus

text: male

characteristic: sex

text: Sprague-Dawley

characteristic: strain

text: Diabetic cardiomyopathy related cDNA


characteristic: External Id

text: Washington University School of Medicine

characteristic: INSDC center name

text: live

text: Generic

text: Bragg

text: The mRNA was isolated from roots of 7 day old 'Bragg' seedlings that were mock-infected 48 hours prior to harvest. Dr. Gary Stacey generously donated the tissue. The roots were flash-frozen in liquid nitrogen. Stratagene's cDNA Synthesis Kit (catalog number 200401) was used to synthesize the cDNA. First-strand synthesis was performed with 5-methyl dCTP, hence the ligated cDNA was hemimethylated. A modification of Stratagene's first-strand synthesis primer was used. An 'anchor' nucleotide (V=A, C, or G) was added to the 3' end of the primer [GAGAGAGAGAGAGAGAGAGAACTAGTCTCGAG(T)18V] to anchor the primer at the 5' end of the poly(A) tract. After second-strand synthesis, the cDNA ends were filled in with cloned Pfu DNA, ligated to EcoRI adapters and subsequently phosphorylated. The cDNA was then precipitated and redissolved in sterile, RNase-, DNase-free water. The XhoI site within the first- strand synthesis primer was then restricted by digestion with XhoI from Promega (40U/ul); all XhoI sites in the cDNA would be protected by their hemimethylated status. The cDNA constructs were size-fractionated with a 500bp cutoff, using Sephacryl S-500 High Resolution (Pharmacia Biotech) in a 2-mm diameter column and a bed volume of approximately 1ml. The column eluent was precipitated, redissolved, and ligated into Stratagene's pBluescript II XR Predigested vector (pBluescript II SK(+) vector that has been digested with EcoRI and XhoI, and phosphorylated by Stratagene). This library was constucted in the laboratory of Dr. Paul Keim and Dr. Virginia H. Coryell at Northern Arizona University.

text: 7 days old

text: Glycine max

text: EcoRI

text: XhoI

text: Roots of 7 day old 'Bragg' seedlings

text: plasmid

text: pBluescript II SK+


characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: The library was constructed by Brandi Chiapelli and Dr. James McCarter at Washington University, St. Louis. The cDNA was made by using Dynabead oligo-dT priming (Dynal). PCR based library using a modified protocol from the SMART PCR cDNA Synthesis Kit from Clontech. Directionally cloned into the UDG sites of pAMP1.

text: mixed

text: Pristionchus pacificus

text: NotI

text: SalI

text: Prist pacificus mixed stage pAMP1 v1 Chiapelli McCarter

text: plasmid (ampicillin resistant)

text: pAMP1 (Gibco)


text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: The library was constructed by Brandi Chiapelli and Dr. James McCarter at Washington University, St. Louis. The cDNA was made by using Dynabead oligo-dT priming (Dynal). PCR based library using a modified protocol from the SMART PCR cDNA Synthesis Kit from Clontech. Directionally cloned into the UDG sites of pAMP1. Nematodes were provided by Dr. Warwick Grant of AgRearch, New Zealand (warwick.grant@agresearch.co.nz). Worms were harvested from Australian brush-tailed possum (Trichosuri vulpecula) and washed thoroughly to remove host contamination. Note that despite this effort, host contamination of the library is possible.

text: Parasitic Adults

text: Parastrongyloides trichosuri

text: NotI

text: SalI

text: Parastrongyloides trichosuri PA pAMP1 v1 Chiapelli McCarter

text: plasmid (ampicillin resistant)

text: pAMP1 (Gibco)


text: live

text: Generic

characteristic: NCBI submission package

text: The library was constructed by Brandi Chiapelli and Dr. James McCarter at Washington University, St. Louis. The cDNA was made by using Dynabead oligo-dT priming (Dynal). PCR based library using a modified protocol from the SMART PCR cDNA Synthesis Kit from Clontech. Directionally cloned into the UDG sites of pAMP1. Nematodes were provided by Dr. David Bird and Daniel Snyder of North Carolina State University.

characteristic: description

text: egg

text: Meloidogyne arenaria

text: NotI

text: SalI

text: Meloidogyne arenaria egg pAMP1 v1 Chiapelli McCarter

text: plasmid (ampicillin resistant)

text: pAMP1 (Gibco)


text: Nanhua University

text: live

text: Generic

text: leukaemia cell

characteristic: cell type

characteristic: cell-line

text: Lambda Zap Express Library

text: Homo sapiens

text: Blood

text: Human leukaemia cell U937 cDNA

text: lambda ZAP


text: IIB-INTECH-CONICET-UNSAM. Instituto de Investigaciones Biotecnologicas, Universidad Nacional de Gral. San Martin

text: live

text: Generic

text: Sinvalocho-MA

text: Clones isolated from a rust-resistant wheat line by mRNA differential display methodology after innoculation with Puccinia recondita

text: leaf

characteristic: organ

text: Triticum aestivum

characteristic: organism

text: EcoRI

text: EcoRI

text: Wheat genes involved in rust resistance

text: plasmid

text: pGEM-T-Easy


text: University of Illinois at Urbana-Champaign

text: live

text: Generic

text: This BB17 cDNA library was generated by subtraction of the BB16 library with 4000 previously sequenced clones. The BB16 library was contributed by the Soares laboratory and it was constructed and normalized as described by Bonaldo, M.F., Lennon, G. and Soares, M.B. (1996), Genome Research 6(9): 791-806. RNA was prepared from dissected brains of adult worker bees of various ages and various behavioral groups.

text: adult worker honey bee

characteristic: lab-host

text: brain

text: Apis mellifera

text: female

text: mixed strains of European bees, predominantly A.m. ligustica

text: brain

characteristic: tissue-type

text: Bee Brain Normalized/Subtracted Library, BB17

characteristic: title

text: Plasmid

characteristic: vector


text: University of Western Ontario

text: live

text: Generic

text: The BB16 library was contributed by the Soares laboratory and it was constructed and normalized as described by Bonaldo, M.F., Lennon, G. and Soares, M.B. (1996), Genome Research 6(9): 791-806. RNA was prepared from dissected brains of adult worker bees of various ages and various behavioral groups.

text: adult worker honey bee

characteristic: dev-stage

text: brain

text: Apis mellifera

text: female

characteristic: sex

characteristic: strain

text: brain

text: Bee Brain Normalized Library, BB16

text: Plasmid


characteristic: External Id

text: Torrey Mesa Research Institute

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: Chenopodium amaranticolor

text: EcoRI

text: MseI

text: tobacco mosaic virus infected leaves

text: cDNA-AFLP


text: Georgia Institute of Technology

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Isolated using differential display PCR.

text: adult

text: Acropora cervicornis

text: Staghorn coral stress responses


text: Harvard University, Howard Hughes Medical Institute

text: live

text: Generic

characteristic: NCBI submission package

text: Library constructed using SuperScript Plasmid Library kit (Life Technologies). cDNA made by oligo-dT priming. Size-selected by column fractionation; average insert size 0.91 kb. Amplified once on solid support. cDNA Library Preparation: Guolin Chen.

characteristic: description

text: Adult

text: Pancreas

text: Mus musculus

text: Not 1

text: Sal 1

text: Male

text: ICR

text: Islets of Langerhans

text: Amplified Melton Mouse Islets 1 MIS1-A

text: plasmid (ampicillin resistant)


text: The Institute of Physical and Chemical Research (RIKEN)

text: live

text: Generic

text: adult

text: Mus musculus

text: male

text: liver

text: RIKEN full-length enriched, adult male liver


text: live

text: Generic

text: 10 day old

text: Mus musculus

text: male

text: pancreas

text: RIKEN full-length enriched, 10 day old male pancreas


text: University of Calcutta

text: live

text: Generic

text: After treating the cells with cigarette smoke, we used differential display technique to find out differentially expressed transcripts.

text: Schizosaccharomyces pombe

text: Schizosaccharomyces pombe differential display


text: Institute of Molecular Biology and Genetics

text: live

text: Generic

text: S. Meier-Ewert's fetal brain cDNA library no.507. 1st strand cDNA was primed with a Not I oligo(dT) primer. Double-stranded cDNA was ligated to Sal I adaptors, digested with Not I and directionally cloned into the Not I and Sal I sites of pSPORT1 vector.

text: fetus week 17 post-fertilization

text: Homo sapiens

text: Sal I

text: Not I

text: brain

text: Human fetal brain cDNA library

text: plasmid


text: Michigan State University

text: live

text: Generic

characteristic: cultivar

text: The mRNA samples used for this library were derived from seedlings germinated for 4 days in 88mM H2O2. The mRNA pool was used as template for double stranded cDNA synthesis using the Stratagene pBluescript XR cDNA synthesis and library kit. The resulting cDNA was used as a tester for subtraction against a driver cDNA population derived from 4-day old seedlings germinated in moist filter paper. Subtraction was performed using the Invitrogen Photobiotin-Streptavidin subtractor kit. The cDNA library was generated by directional ligation of the subtracted cDNAs in the EcoRI and XhoI sites of pBluescript XR vector (Stratagene).

text: 4-day germination in 88mM H2O2

text: XL1-Blue MRF'

text: seeds

characteristic: organ

text: Beta vulgaris

text: EcoRI

text: XhoI

text: whole seedlings

text: Sugar Beet peroxide germination cDNA library (subtracted)

text: plasmid

text: pBluescript II KS (+)


text: Michigan State University

text: live

text: Generic

text: cDNAs were derived from reverse transcription of mRNA samples from immature inflorescence. The mRNA pool was used as template for double stranded cDNA synthesis using the Stratagene pBluescript XR cDNA synthesis and library kit. The resulting cDNA was used as a tester for subtraction against a driver cDNA population derived from root, leaf and 4-day old germinated seedlings. Subtraction was performed using the Invitrogen Photobiotin-Streptavidin subtractor kit. The cDNA library was generated by directional ligation of the subtracted cDNAs in the EcoRI and XhoI sites of pBK-CMV vector (Stratagene).

text: XLOLR

characteristic: lab-host

text: inflorescence

text: Beta vulgaris

characteristic: organism

text: EcoRI

text: XhoI

text: whole inflorescence

text: Sugar Beet inflorescence cDNA library (subtracted)

text: plasmid

text: pBK-CMV


text: Ocean University of Qingdao

text: live

text: Generic

text: Wild type Gracilaria lemaneiformis were harvested from Zhanshan Bay, Qingdao (China). After rinsed with boiled seawater, younger thalli was cut and washed every 3-4 days until 1 month. Then thalli was cultivated in Provasoli medium. Total RNA was isolated from thalli of gametophyte algae, using UNIQ-10 Trizol Total RNA Preparation Kit(Sangon Inc., Shanghai, China). The cDNA was synthesized, amplified and cloned using cDNA Synthesis Kit, cDNA PCR Library Kit and pMD 18-T vector (TaKaRa Biotechnology Co., Ltd., Dalian, China), respectively.

text: Gracilariopsis lemaneiformis

text: EcoR V with a T hang

text: gametophyte thalli

characteristic: tissue-type

text: Gracilaria lemaneiformis gametophyte cDNA library

characteristic: title

text: Plasmid

text: pMD 18-T


text: Yonsei Univ., Wonju College of Medicine

text: live

text: Generic

text: hyphae formation

characteristic: dev-stage

text: Candida albicans

text: Candida albicans hyphae formation


text: Chinese PLA General Hospital

text: live

text: Generic

text: Homo sapiens

text: stomach

text: Homo sapiens stomach


text: Southwest Hospital, Third Military Medical University

text: live

text: Generic

text: dermal papillae

characteristic: cell type

text: cDNA was made by SMART PCR cDNA synthesis kit (Clontech). Subtractive library was established using subtractive hybridization technique in order to clone genes related to alopecia areata.

text: alopecia areata

text: skin

text: Homo sapiens

text: male

characteristic: sex

text: scalp

text: Dermal Papillae cDNA Library from alopecia areata patient

text: Cosmid

text: pUCm

characteristic: vector


characteristic: External Id

text: The Volcani Center

characteristic: INSDC center name

text: live

text: Generic

text: country=Israel

text: Solanum lycopersicum

text: stamens and petals

text: pT-Adv T/A mixed flowers from pre-anthesis to 3 days post anthesis


text: The Volcani Center

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: country=Israel

text: parthenocarpic young (4-6mm) fruit

text: Solanum lycopersicum

text: pT-Adv T/A parthenocarpic young (4-6mm) fruit


text: School of Animal Science and Veterinary Medicine

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: adult

text: Sus scrofa

text: Erhualian

characteristic: strain

text: skeletal muscle, M. longissimus dorsi

text: Sus scrofa skeletal muscle, M. longissimus dorsi (Li,K.)


text: Institute of Tropical Medicine

text: live

text: Generic

characteristic: NCBI submission package

text: hemocytes

text: Insert was amplified using T3 and T7 vector specific primer. Total RNA was isolated from the hemocytes of unexposed Biomphalaria glabrata (13-16-R1) snails and first strand cDNA synthesized using anchored oligo-(dT)-primers (H-T11-A/C/G; GenHunter,Nashville,TN). 33P-labeled second strand synthesis was carried out using one of thirty two 13-mer arbitrary primers (GenHunter,Nashville,TN) in a PCR based amplification reaction. The labeled PCR products were separated using 6% polyacrylamide/8M urea gels and the differentially expressed fragments identified by autoradiography. The different bands of interest were sliced out from the gel and the DNA was extracted. This DNA was reamplified using the identical primer combination and differential expression was confirmed by SSCP. The PCR-product was cloned into the plasmid vector pCR4-TOPO (Invitrogen,Groningen,Netherlands) and then subjected to sequencing.

characteristic: description

text: adult

text: E.coli

text: Biomphalaria glabrata

text: hermaphrodite

text: Biomphalaria glabrata 13-16-R1 hemocytes

text: Plasmid


text: Institute of Tropical Medicine

text: live

text: Generic

text: hemocytes

text: Insert was amplified using T3 and T7 vector specific primer. Total RNA was isolated from the hemocytes of unexposed Biomphalaria glabrata (M-line) snails and first strand cDNA synthesized using anchored oligo-(dT)-primers (H-T11-A/C/G; GenHunter,Nashville,TN). 33P-labeled second strand synthesis was carried out using one of thirty two 13-mer arbitrary primers (GenHunter,Nashville,TN) in a PCR based amplification reaction. The labeled PCR products were separated using 6% polyacrylamide/8M urea gels and the differentially expressed fragments identified by autoradiography. The different bands of interest were sliced out from the gel and the DNA was extracted. This DNA was reamplified using the identical primer combination and differential expression was confirmed by SSCP. The PCR-product was cloned into the plasmid vector pCR4-TOPO (Invitrogen,Groningen,Netherlands) and then subjected to sequencing.

text: adult

text: E.coli

characteristic: lab-host

text: Biomphalaria glabrata

text: hermaphrodite

text: M-line

text: Biomphalaria glabrata M-line hemocytes

text: Plasmid


text: Michigan State University

text: live

text: Generic

text: The cDNA library was made from the cambium and bark region of a mature black locust tree collected in Michigan in late July.

text: mature tree

text: Robinia pseudoacacia

characteristic: organism

text: Sfi IA

text: Sfi IB

text: cambial region (Cambium and bark region of black locust - Summer)

text: CLS (Cambium and bark region of black locust - Summer)

text: Phage

text: lambda TriplEx


text: Michigan State University

text: live

text: Generic

text: The cDNA library was made from the sapwood of a mature black locust tree collected in Michigan in late July.

text: mature tree

text: Robinia pseudoacacia

text: Sfi IA

text: Sfi IB

text: sapwood

characteristic: tissue-type

text: SWS (Sapwood of black locust - Summer)

text: Phage

text: lambda TriplEx


text: Michigan State University

text: live

text: Generic

text: The cDNA library was made from the sapwood-heartwood transition zone of a mature black locust tree collected in Michigan in late November.

text: mature tree

text: Robinia pseudoacacia

text: Sfi IA

text: Sfi IB

text: sapwood-heartwood transition zone

text: TZF (Sapwood-heartwood transition zone of black locust - Fall)

characteristic: title

text: Phage

text: lambda TriplEx


text: Michigan State University

text: live

text: Generic

text: The cDNA library was made from the sapwood-heartwood transition zone of a mature black locust tree collected in Michigan in late July.

text: mature tree

characteristic: dev-stage

text: Robinia pseudoacacia

text: Sfi IA

text: Sfi IB

text: sapwood-heartwood transition zone

text: TZS (Sapwood-heartwood transition zone of black locust - Summer)

text: Phage

text: lambda TriplEx


text: Michigan State University

text: live

text: Generic

characteristic: cultivar

text: cDNAs were derived from reverse transcription of mRNA samples from mature root tissue. The mRNA pool was used as template for double stranded cDNA synthesis using the Stratagene pBluescript XR cDNA synthesis and library kit. The resulting cDNA was used as a tester for subtraction against a driver cDNA population derived from leaf, inflorescence, and 4-day old germinated seedlings. Subtraction was performed using the Invitrogen Photobiotin-Streptavidin subtractor kit. The cDNA library was generated by directional ligation of the subtracted cDNAs in the EcoRI and XhoI sites of pBK-CMV vector (Stratagene). Putative ID reported when E scores were better than e-10 via nr database and blastx.

text: XLOLR

text: root

characteristic: organ

text: Beta vulgaris

text: EcoRI

text: XhoI

text: mature root

text: Sugar beet root cDNA library (subtracted)

text: plasmid

text: pBK-CMV


text: University of Bonn

text: live

text: Generic

text: cDNA fragments obtained by heterologous RT-PCR

text: liver

text: Sus scrofa

text: heterologous RT-PCR


text: NC State University; IACR-Rothamsted

text: live

text: Generic

text: This is a collaborative effort between IACR-Rothamsted and North Carolina State University. The library was constructed from mixed stage G. pallida in lambda GT11 by Paul Burroughs, IACR-Rothamsted.

text: Globodera pallida

text: Mixed Stage EST's from Globodera pallida, the potato cyst nematode

text: lambda GT11

characteristic: vector


characteristic: External Id

text: MD Anderson Cancer Center, University of Texas

text: live

text: Generic

text: cloned by RT-PCR

text: Homo sapiens

text: Human SPRL genes


text: Washington University School of Medicine

characteristic: INSDC center name

text: live

text: Generic

text: Library was constructed by Debopam Chakrabarti. Total RNA samples were isolated from mixed stage saponin(0.1%)-lysed P. falciparum 3D7 infected erythrocytes by the acidic guanidinium-phenol chloroform method. The poly A+ RNA was isolated by the polyAT-Tract mRNA isolation system (Promega, WI) using streptavidin Magnesphere particles. Directional cDNA libraries were constructed by oligo d(T) priming of poly(A)+ RNA (5mg) into EcoRI and XhoI sites of l ZapII vector using the Zap cDNA synthesis kit (Stragene, CA). The average size of the cDNA inserts in the library was between 1.0 and 1.5kb. Clones were mass excised using the ExAssist helper phage (Stratagene), the phagemids were precitptated with PEG 8000 and extracted with phenol/chloroform. Phagemid DNA was electroporated into DH10B cells.

text: DH10B (GeneHog, Invitrogen, Inc.)

text: Plasmodium falciparum 3D7

text: EcoRI

text: XhoI

text: Plasmodium falciparum 3D7 asexual cDNA

text: phagemid (ampicillin resistant)

text: pBluescript SK plus


characteristic: INSDC first public

text: live

text: Generic

text: First strand cDNA synthesis was primed using oligo-dT on magnetic beads with an additional primer 5'-gcggccgctaatacgactcacta-taggg-3'. Second strand synthesis was a 3-cycle PCR using the primers 5'-ggccgctaatacgactcactatag-3' and 5'-aagcagtggtaacaacgcagagtacttt-ttttttttttttvn-3'. cDNA was subsequently amplified in a 7-cycle PCR with the following primers: 5'-ggccgctaatacgactcactatag-3' and 5'-aagcagtggt-aacaacgcag. Deoxy-UMP adaptors were added in a third PCR (5 cycles) and the primers 5'-caucaucaucauggccgctaatacgactcactataggg-3' and 5'-cuacuacuacuaaagcagtggtaacaacgcagagtac-3'. Ends were treated with uracil DNA glycosylase and product with 3' overhangs was annealed to complementary ends of pAMP1. Insert can be excised using EcoRI and NotI. Library constructed by Joe Barnes and Steve Johnson (Washington University).

text: Danio rerio

text: EcoRI

text: NotI

text: male

characteristic: sex

text: fin, 8-day regeneration

text: Zebrafish SJD day 8 fin regeneration

text: phagemid (ampicillin resistant)


text: Lawrence Livermore National Laboratory

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Poly A+ RNA was isolatd from the ovaries of 2 female adult zebrafish (4-5 month old). cDNAs were made using oligo-dT primers and inserted into lambda ZAP II vector (Stratagene) by Dr. Z. Gong, in vivo mass-excised to pBluescript SK- following the Washington University protocol (http://genome.wustl.edu/est/lambda_protocol.shtml). Please contact Zhiyuan Gong for further information on this library (National University of Singapore, Department of Biological Sciences, Lower Kent Ridge Road, Singapore 119260).

text: 4-5 month

text: DH10B (phage-resistant)

text: ovary (pooled)

text: Danio rerio

text: XhoI

text: EcoRI

text: female

text: Gong zebrafish ovary

text: phagemid (ampicillin resistant)

text: pBluescript SK-


text: live

text: Generic

characteristic: NCBI submission model

text: Poly A+ RNA was isolatd from the testes of 31 male adult zebrafish (4-5 month old). cDNAs were made using oligo-dT primers and inserted into lambda ZAP II vector (Stratagene) by Dr. Z. Gong, in vivo mass-excised to pBluescript SK- following the Washington University protocol (http://genome.wustl.edu/est/lambda_protocol.shtml). Please contact Zhiyuan Gong for further information on this library (National University of Singapore, Department of Biological Sciences, Lower Kent Ridge Road, Singapore 119260).

text: 4-5 month

text: DH10B (phage-resistant)

text: testis (pooled)

text: Danio rerio

text: XhoI

text: EcoRI

text: male

text: Gong zebrafish testis

text: phagemid (ampicillin resistant)

text: pBluescript SK-


text: University of Illinois

text: live

text: Generic

characteristic: NCBI submission package

text: Developmental stage

text: Zea mays

text: Embryo

text: B73 LIBRARY

text: Plasmid


text: The Third Hospital of Peking University

text: live

text: Generic

text: expressed higher in hypertrophic scar than that in keloid

characteristic: description

text: hypertrophic scar

text: Homo sapiens

text: female

text: skin

text: Homo sapiens hypertrophic scar cDNA


text: live

text: Generic

text: Minsoy x Noir RI progeny

text: The mRNA was isolated from roots of bulked Minsoy x Noir RI progeny that are phenotypically susceptible to flooding damage. The plants were flooded (submerged) for 24 hours prior to harvest. Dr. Tara VanToai generously provided the isolated mRNA. The roots were flash- frozen in liquid nitrogen. Stratagene's cDNA Synthesis Kit (catalog number 200401) was used to synthesize the cDNA. First-strand synthesis was performed with 5-methyl dCTP, hence the ligated cDNA was hemimethylated. A modification of Stratagene's first-strand synthesis primer was used. An 'anchor' nucleotide (V=A, C, or G) was added to the 3' end of the primer [GAGAGAGAGAGAGAGAGAGAACTAGTCTCGAG(T)18V] to anchor the primer at the 5' end of the poly(A) tract. After second-strand synthesis, the cDNA ends were filled in with cloned Pfu DNA, ligated to EcoRI adapters and subsequently phosphorylated. The cDNA was then precipitated and redissolved in sterile, RNase-, DNase-free water. The XhoI site within the first- strand synthesis primer was then restricted by digestion with XhoI from Promega (40U/ul); all XhoI sites in the cDNA would be protected by their hemimethylated status. The cDNA constructs were size-fractionated with a 500bp cutoff, using Sephacryl S-500 High Resolution (Pharmacia Biotech) in a 2-mm diameter column and a bed volume of approximately 1ml. The column eluent was precipitated, redissolved, and ligated into Stratagene's pBluescript II XR Predigested vector (pBluescript II SK(+) vector that has been digested with EcoRI and XhoI, and phosphorylated by Stratagene). This library was constucted in the laboratory of Dr. Paul Keim and Dr. Virginia H. Coryell at Northern Arizona University.

characteristic: lab-host

text: Glycine max

text: EcoRI

text: XhoI

text: Roots of bulked Minsoy x Noir RI progeny

text: plasmid

text: pBluescript II SK+


text: University of Georgia

text: live

text: Generic

text: 3'-RACE product from reactions using a degenerate primer for members of the iron-ascorbate oxidase superfamily in plants

text: elongating shoots

text: Pinus taeda

characteristic: organism

text: compression wood

text: loblolly pine bent shoot tip


text: Max-Planck-Institut fuer Molekulare Genetik

text: live

text: Generic

text: oligo-dT-NotI primed, SalI adaptors, directionally cloned, library normalised by oligonucleotide fingerprinting

text: shield stage, 6 hrs post-fertilisation

text: E.coli, XL1 blue MRF

text: Danio rerio

text: NotI

text: SalI

text: whole embryo

characteristic: tissue-type

text: Zebrafish shield stage whole embryo cDNA library MPMGp637

text: Plasmid


text: IGBMC

text: live

text: Generic

text: directly subcloned fragments resulting from the nested PCR of the Clontech PCRSelect subtraction procedure

text: shield stage, 6 hrs post-fertilisation

text: TOP10 OneShot cells

text: Danio rerio

text: whole embryo

text: Zebrafish shield stage whole embryo

characteristic: title

text: Plasmid

text: pCRII-TOPO


text: live

text: Generic

text: SL1 PCR-based library. Ancylostoma ceylanicum cDNA PCR products of size nucleotides containing SL1 on the 5' end and oligo(dT) on the 3' end were non-directionally cloned into pCR-XL-TOPO (Invitrogen) following the cloning protocol. The cDNA insert can be excised by digestion with EcoRI. The library was constructed by Dr. Vadim Kapulkin from the University of Colorado at Boulder.

text: adults

characteristic: dev-stage

text: Ancylostoma ceylanicum

text: EcoRI

text: EcoRI

text: mixed

text: Ancylostoma ceylanicum M1 SL1 TOPO Kapulkin

text: Plasmid (kanamycin resistant)

text: pCR-XL-TOPO (Invitrogen)


text: Russian Academy of Sciences

text: live

text: Generic

text: B-cell

characteristic: cell type

text: SIV-associated centroblastic non-Hodgkin's lymphoma

text: Macaca mulatta

text: Macaca mulatta SIV-associated centroblastic non-Hodgkins lymphoma

text: pBluescript SK


text: Duke University

text: live

text: Generic

text: Stress condition II library, constructed by John Davies and Jeffrey McDermott, combines cDNAs from CC-1690 cells grown to mid-log phase in TAP (NH4+ - containing) and shifted to TAP - NO3- (24hrs); H2 production conditions (0, 12hr, 24hr) see Melis et al.,(2000) Plant Phys. 122: 127-135; TAP + H2O2 (1, 12, 24 hr); TAP + sorbitol (1, 2, 6, 24 hr); TAP + Cd (1, 2, 6, 24 hr). PolyA mRNA was purified from each sample, pooled and cDNA synthesized. The cDNA was directionally cloned into lambda Zap II (Stratagene) in the EcoRI (5') and XhoRI (3') sites. pBluescript II SK- plasmids were excised from the lambda ZAP clones by superinfection with ExAssist (Stratagene) phage. The library was normalized using method 4 described in Bonaldo et al., (1996) Genome Research 6: 791-806.

text: Chlamydomonas reinhardtii

text: EcoRI

text: XhoI

text: CC-1690 wild type mt+ 21gr

characteristic: strain

text: C. reinhardtii CC-1690, Stress II (normalized), Lambda Zap II

text: plasmid

text: pBluescript II SK-


text: Rubber Research Institute of Malaysia

text: live

text: Generic

text: laticifers

text: RT-PCR fragment of latex B-serum beta-glucosidase synthesized using degenerate sense and antisense primers derived from consensus region in plant beta-glucosidases

text: latex

text: Hevea brasiliensis

text: vascular

text: Hevea brasiliensis RT-PCR fragment


text: Kyoto University

text: live

text: Generic

text: Bos taurus

text: placenta

text: Bovine placenta cDNA


text: Institute for Systems Biology

text: live

text: Generic

text: Unamplified, non-normalized cDNA library in pSPORT prepared from a pool of RNAs from 5 metastatic ovarian carcinomas. Clones were PCR amplified using the forward primer GTGAGCGGATAACAATTTCAC and the reverse primer TTTCCCAGTCACGACGTTGTA and 5' sequenced with M13 reverse primer.

text: adult

text: E.coli

text: ovary

characteristic: organ

text: Homo sapiens

text: female

text: metastatic cancer

text: T000M, Human Metastatic Ovarian Cancer

text: Plasmid

text: pSPORT

characteristic: vector


characteristic: External Id

text: Warsaw University of Life Sciences

characteristic: INSDC center name

text: live

text: Generic

text: Borszczagowski

characteristic: cultivar

text: The embryogenic suspension culture was induced towards SE by removal of 2,4-D from the medium. mRNA was isolated from tissue before induction and 2, 6, 12, 24 hours, 3, 7 and 14 days after induction.

text: 0- 14 days after induction of somatic embryogenesis

text: E. coli

text: Cucumis sativus

text: HindIII

text: HindIII

text: embryogenic suspension culture

text: cucumber DD-RTPCR cDNA fragments

text: Plasmid

text: PCR-Trap (Gene Hunter)


text: Warsaw University of Life Sciences

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Borszczagowski

text: E. coli

text: Cucumis sativus

text: RsaI

text: embryogenic suspension culture

text: cucumber SSH cDNA fragments

text: Plasmid

text: pBluescript KS (+)


text: Clemson University

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Spontaneous mutant of unknown origin

text: Early vegetative development

text: Apical meristems

text: Glycine max

text: Shoot apex cDNA library

text: Phage

text: LambdaZapII


text: Beijing Institute of Transfusion Medicine

text: live

text: Generic

characteristic: NCBI submission package

text: Fetal

text: Homo sapiens

text: Kidney

text: Human fetal kidney subtracted cDNA library

text: pT-Adv


text: Washington University School of Medicine

text: live

text: Generic

text: The library was constructed by Brandi Chiapelli and Dr. James McCarter at Washington University, St. Louis. The cDNA was made by using Dynabead oligo-dT priming (Dynal). PCR based library using a modified protocol from the SMART PCR cDNA Synthesis Kit from Clontech. Directionally cloned into the UDG sites of pAMP1. Nematodes were provided by Dr. Prema Arasu of North Carolina State University.

characteristic: description

text: serum stimulated L3

characteristic: lab-host

text: Ancylostoma caninum

text: NotI

text: SalI

text: Anc caninum L3 serum stim pAMP1 v1 Chiapelli McCarter

text: plasmid (ampicillin resistant)

text: pAMP1 (Gibco)


text: University of Guelph

text: live

text: Generic

text: Subtraction products were cloned in pCR2.1-TOPO vector

text: Three-week-old plants

text: Solanum lycopersicum

characteristic: organism

text: Stem below the first node

characteristic: tissue-type

text: Subtraction hybridization products


text: Agriculture and Agri-food Canada

text: live

text: Generic

text: Mycelial tissue was collected from V8 agar plates after a growth period of 6-7 days at 25 C with 14 hrs (FL/UV) day ligth exposure. Mycelia was ground in liquid nitrogen prior it's storage at -80 C until RNA extraction. Directional cloning with 5' end of cDNA cloned into EcoRI site of pBluescript and 3' end of cDNA cloned into XhoI site of pBluescript (Stratagene,La Jolla, CA).

text: Assexual

characteristic: dev-stage

text: E. coli (Sure cells)

text: Fusarium graminearum

text: EcoRI

text: XhoI

text: DAOM 180378

text: Mycelial tissue

text: Fg02_AAFC_ECORC_Fusarium_graminearum_mycelium

characteristic: title

text: Plasmid

text: Bluescript SK+/XhoI-EcoRI


text: Agriculture and Agri-food Canada

text: live

text: Generic

text: Frontana

text: Controled chamber-grown wheat heads were spray inoculated at mid-anthesis with a Fusarium graminearum macroconidial suspension (50,000 spores/ml) and kept under intermitent misting for 24 hours, then collected and immediately frozen in liquid nitrogen.

text: anthesis

text: Triticum aestivum

text: EcoRI

text: heads

text: Ta01_AAFC_ECORC_Fusarium_graminearum_inoculated_wheat_heads

text: Plasmid

text: pGEM-T easy


text: live

text: Generic

text: Fetal

text: Homo sapiens

text: Bone marrow

text: Homo sapiens fetal bone marrow mesenchymal stem cells subtracted cDNA library

text: pGEM-T


text: Scottish Crop Research Institute

text: live

text: Generic

text: Optic

text: Non-normalised library, directionally cloned into pSPORT1. Derived from embryos dissected from developing grains (12 days post anthesis) in glasshouse grown barley plants. Developed as part of the barley transcriptome resources of BBSRC/SEERAD funded cereal IGF (Investigating Gene Function) project.

text: 12 DPA

text: Hordeum vulgare subsp. vulgare

text: Sal I

text: Not I

text: embryo

text: embryo, 12 DPA, no treatment, cv Optic, EBem04

text: Plasmid


text: Scottish Crop Research Institute

text: live

text: Generic

text: Optic

text: Non-normalised library, directionally cloned into pSPORT1. Derived from pistils dissected from developing grains (8 days post anthesis) in glasshouse grown barley plants. Developed as part of the barley transcriptome resources of BBSRC/SEERAD funded cereal IGF (Investigating Gene Function) project.

text: 8 DPA

text: Hordeum vulgare subsp. vulgare

text: Sal I

text: Not I

text: pistil

text: pistil, 8 DPA, no treatment, cv Optic, EBpi05

text: Plasmid


text: Scottish Crop Research Institute

text: live

text: Generic

text: Optic

text: Non-normalised library, directionally cloned into pSPORT1. Derived from pistils dissected from developing grains (12 days post anthesis) in glasshouse grown barley plants. Developed as part of the barley transcriptome resources of BBSRC/SEERAD funded cereal IGF (Investigating Gene Function) project.

text: 12 DPA

text: Hordeum vulgare subsp. vulgare

text: Sal I

text: Not I

text: pistil

text: pistil, 12 DPA, no treatment, cv Optic, EBpi07

text: Plasmid


text: Scottish Crop Research Institute

text: live

text: Generic

text: Optic

text: Non-normalised library, directionally cloned into pSPORT1. Derived from pistils dissected from developing grains (4 days post anthesis) in glasshouse grown barley plants. Developed as part of the barley transcriptome resources of BBSRC/SEERAD funded cereal IGF (Investigating Gene Function) project.

text: 4 DPA

text: Hordeum vulgare subsp. vulgare

text: Sal I

text: Not I

text: pistil

text: pistil, 4 DPA, no treatment, cv Optic, EBpi03

text: Plasmid


text: Scottish Crop Research Institute

text: live

text: Generic

text: Optic

text: Non-normalised library, directionally cloned into pSPORT1. Derived from roots of 3 week old waterlogged barley plants. Developed as part of the barley transcriptome resources of BBSRC/SEERAD funded cereal IGF (Investigating Gene Function) project.

text: 3 week

text: Hordeum vulgare subsp. vulgare

text: Sal I

text: Not I

text: root

text: root, 3 week, waterlogged, cv Optic, EBro03

text: Plasmid


text: Scottish Crop Research Institute

text: live

text: Generic

text: Optic

text: Non-normalised library, directionally cloned into pSPORT1. Derived from roots of 3 week old salt stressed barley plants. Developed as part of the barley transcriptome resources of BBSRC/SEERAD funded cereal IGF (Investigating Gene Function) project.

text: 3 week

text: Hordeum vulgare subsp. vulgare

text: Sal I

text: Not I

text: root

text: root, 3 week, salt-stressed, cv Optic, EBro04

text: Plasmid


text: Scottish Crop Research Institute

text: live

text: Generic

text: Optic

text: Non-normalised library, directionally cloned into pSPORT1. Derived from roots of 3 week old drought stressed barley plants. Developed as part of the barley transcriptome resources of BBSRC/SEERAD funded cereal IGF (Investigating Gene Function) project.

text: 3 week

text: Hordeum vulgare subsp. vulgare

text: Sal I

text: Not I

text: root

text: root, 3 week, drought-stressed, cv Optic, EBro08

text: Plasmid


text: Scottish Crop Research Institute

text: live

text: Generic

text: Optic

text: Non-normalised library, directionally cloned into pSPORT1. Derived from shoot and root material of 3 week old etiolated barley plants. Developed as part of the barley transcriptome resources of BBSRC/SEERAD funded cereal IGF (Investigating Gene Function) project.

text: 3 week

text: Hordeum vulgare subsp. vulgare

text: Sal I

text: Not I

text: root

text: root, 3 week, reduced light, cv Optic, EBro07

text: Plasmid


text: Scottish Crop Research Institute

text: live

text: Generic

text: Optic

text: Non-normalised library, directionally cloned into pSPORT1. Derived from roots of 3 week old hydroponically grown unstressed barley plants. Developed as part of the barley transcriptome resources of BBSRC/SEERAD funded cereal IGF (Investigating Gene Function) project.

text: 3 week

text: Hordeum vulgare subsp. vulgare

text: Sal I

text: Not I

text: root

text: root, 3 week, hydroponic grown, no treatment, cv Optic, EBro01

text: Plasmid


text: Scottish Crop Research Institute

text: live

text: Generic

text: Optic

text: Non-normalised library, directionally cloned into pSPORT1. Derived from embryos dissected from developing grains (14 days post anthesis) in glasshouse grown barley plants. Developed as part of the barley transcriptome resources of BBSRC/SEERAD funded cereal IGF (Investigating Gene Function) project.

text: 14 DPA

text: Hordeum vulgare subsp. vulgare

text: Sal I

text: Not I

text: embryo

text: embryo, 14 DPA, no treatment, cv Optic, EBem05

text: Plasmid


text: Scottish Crop Research Institute

text: live

text: Generic

text: Optic

text: Non-normalised library, directionally cloned into pSPORT1. Derived from embryos dissected from developing grains (28 days post anthesis) in glasshouse grown barley plants. Developed as part of the barley transcriptome resources of BBSRC/SEERAD funded cereal IGF (Investigating Gene Function) project.

text: 28 DPA

text: Hordeum vulgare subsp. vulgare

text: Sal I

text: Not I

text: embryo

text: embryo, 28 DPA, no treatment, cv Optic, EBem07

text: Plasmid


text: Scottish Crop Research Institute

text: live

text: Generic

text: Optic

text: Non-normalised library, directionally cloned into pSPORT1. Derived from maternal tissue dissected from developing grains (28 days post anthesis) in glasshouse grown barley plants. Developed as part of the barley transcriptome resources of BBSRC/SEERAD funded cereal IGF (Investigating Gene Function) project.

text: 28 DPA

text: Hordeum vulgare subsp. vulgare

text: Sal I

text: Not I

text: maternal

text: maternal, 28 DPA, no treatment, cv Optic, EBma08

text: Plasmid


text: Scottish Crop Research Institute

text: live

text: Generic

text: Optic

text: Non-normalised library, directionally cloned into pSPORT1. Derived from embryos dissected from developing grains (21 days post anthesis) in glasshouse grown barley plants. Developed as part of the barley transcriptome resources of BBSRC/SEERAD funded cereal IGF (Investigating Gene Function) project.

text: 21 DPA

text: Hordeum vulgare subsp. vulgare

text: Sal I

text: Not I

text: embryo

text: embryo, 21 DPA, no treatment, cv Optic, EBem06

text: Plasmid


text: Scottish Crop Research Institute

text: live

text: Generic

text: Optic

text: Non-normalised library, directionally cloned into pSPORT1. Derived from embryos dissected from developing grains (40 days post anthesis) in glasshouse grown barley plants. Developed as part of the barley transcriptome resources of BBSRC/SEERAD funded cereal IGF (Investigating Gene Function) project.

text: 40 DPA

text: Hordeum vulgare subsp. vulgare

text: Sal I

text: Not I

text: embryo

text: embryo, 40 DPA, no treatment, cv Optic, EBem08

text: Plasmid


text: Scottish Crop Research Institute

text: live

text: Generic

text: Optic

text: Non-normalised library, directionally cloned into pSPORT1. Derived from embryo sacs dissected from developing grains (4-6 days post anthesis) in glasshouse grown barley plants. Developed as part of the barley transcriptome resources of BBSRC/SEERAD funded cereal IGF (Investigating Gene Function) project.

text: 4-6 DPA

text: Hordeum vulgare subsp. vulgare

text: Sal I

text: Not I

text: embryo sac

text: embryo sac, 4-6 DPA, no treatment, cv Optic, EBes01

text: Plasmid


text: Scottish Crop Research Institute

text: live

text: Generic

text: Optic

text: Non-normalised library, directionally cloned into pSPORT1. Derived from maternal tissue dissected from developing grains (12 days post anthesis) in glasshouse grown barley plants. Developed as part of the barley transcriptome resources of BBSRC/SEERAD funded cereal IGF (Investigating Gene Function) project.

text: 12 DPA

text: Hordeum vulgare subsp. vulgare

text: Sal I

text: Not I

text: maternal

text: maternal, 12 DPA, no treatment, cv Optic, EBma05

text: Plasmid


text: Scottish Crop Research Institute

text: live

text: Generic

text: Optic

text: Non-normalised library, directionally cloned into pSPORT1. Derived from maternal tissue dissected from developing grains (8 days post anthesis) in glasshouse grown barley plants. Developed as part of the barley transcriptome resources of BBSRC/SEERAD funded cereal IGF (Investigating Gene Function) project.

text: 8 DPA

text: Hordeum vulgare subsp. vulgare

text: Sal I

text: Not I

text: maternal

text: maternal, 8 DPA, no treatment, cv Optic, EBma03

text: Plasmid


text: Washington University School of Medicine

text: live

text: Generic

text: The library was constructed by Brandi Chiapelli and Dr. James McCarter at Washington University, St. Louis. The cDNA was made by using Dynabead oligo-dT priming (Dynal). PCR based library using a modified protocol from the SMART PCR cDNA Synthesis Kit from Clontech. Directionally cloned into the UDG sites of pAMP1. Dissected nematode tissues were provided by Dr. Alan Scott (ascott@jhsph.edu) of the School of Public Hygene and Public Health at John Hopkins University in Baltimore, MD.

text: Adult

text: Ascaris suum

text: NotI

text: SalI

text: Female

characteristic: sex

text: Dissected female gonad (MZ=maturation zone, most proximal region of gonad)

text: Ascaris suum female gonad MZ pAMP1 v2 Chiapelli McCarter

text: plasmid (ampicillin resistant)

text: pAMP1 (Gibco)


text: live

text: Generic

text: Adult

text: Ascaris suum

text: NotI

text: SalI

text: Female

text: Head

text: Ascaris suum female head pAMP1 v2 Chiapelli McCarter

text: plasmid (ampicillin resistant)

text: pAMP1 (Gibco)


text: live

text: Generic

text: Adult

text: Ascaris suum

text: NotI

text: SalI

text: Male

text: Intestine

text: Ascaris suum male gut pAMP1 v2 Chiapelli McCarter

text: plasmid (ampicillin resistant)

text: pAMP1 (Gibco)


text: live

text: Generic

text: Adult

text: Ascaris suum

text: NotI

text: SalI

text: Male

text: Head

text: Ascaris suum male head pAMP1 v2 Chiapelli McCarter

text: plasmid (ampicillin resistant)

text: pAMP1 (Gibco)


text: School of Medicine, Kagoshima University

text: live

text: Generic

text: adipocyte

characteristic: cell type

characteristic: cell-line

text: Mus musculus

text: Mus musculus 3T3-L1 adipocyte


characteristic: External Id

text: Chulalongkorn University

text: live

text: Generic

text: haemocytes

text: Adult

text: Penaeus monodon

text: Shrimp Lambda Zip Express Library

text: Phage

text: Lambda Zip Lox

characteristic: vector


text: Beijing University

characteristic: INSDC center name

text: live

text: Generic

text: Homo sapiens

text: Human cancer


text: Beijing University

characteristic: INSDC first public

text: live

text: Generic

text: Homo sapiens

text: Human normal tissue


text: Beijing University

characteristic: INSDC last update

text: live

text: Generic

text: Homo sapiens

text: suppression subtractive hybridization library of human cancer


text: University of New Mexico

text: live

characteristic: INSDC status

text: Generic

text: mRNA was collected from one M-line Biomphalaria glabrata, 24 hours after wounding with a hypodermic needle. RT-PCR was performed with random primers using methods described by Dias-Neto et al., (1997) GENE 186:135-142. The resulting cDNA fragments were cloned and sequenced.

text: adult

text: Biomphalaria glabrata

text: EcoRI

text: EcoRI

text: hermaphrodite

text: M-line

characteristic: strain

text: whole body

text: CAMWO (C Adema, M-line, wounded, orestes)

text: plasmid


text: The Institute of Molecular Biology

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Homo sapiens

text: brain

text: Homo sapiens SH-SY5Y


text: live

text: Generic

text: Williams 82

characteristic: cultivar

text: The cDNA library was contructed by M. Bhattacharyya from mRNA isolated from etiolated hypocotyls from the cultivar Williams 82. Tissue was inoculated with Phytophthora soyae race 1 and tissues were harvested 2 and 4 hours following infection. The library is the pool of these two time points. Complementary DNA was synthesized from mRNA using a primer consisting of a poly(dT) sequence with a XhoI restriction site. EcoRI adapters were ligated to the blunt-ended cDNA fragments followed by XhoI digestion. The cDNA fragments were directionally cloned into the EcoRI-XhoI restriction site of the pBluescript vector. The ligated cDNA fragments were transformed into DH10B host cells (Gibco BRL). This library was constructed by M. Bhattacharyya in the laboratory of Dr. Randy Shoemaker at Iowa State University.

characteristic: description

characteristic: lab-host

text: Glycine max

text: EcoRI

text: XhoI

text: Etiolated hypocotyls (Williams 82)

text: plasmid

text: pBluescript II SK+


text: Harvard University, Howard Hughes Medical Institute

text: live

text: Generic

text: Starting library constructed using SuperScript Plasmid Library kit (Life Technologies). cDNA made by oligo-dT priming. Size-selected by column fractionation; average insert size 1.08 kb. Library was amplified once on solid support and plasmid DNA from library was prepared. The library DNA was normalized by method #4 from Bonaldo, Lennon, and Soares 1996 Genome Research 6:791-806; 0.5 microgram single-stranded library plasmid DNA was mixed with 5 micrograms PCR product representing library inserts and hybridized to an Ecot of 20. Single-stranded (unhybridized) plasmids were isolated by hydroxyapatite chromatography and used to make this library.

text: Adult

characteristic: dev-stage

text: Pancreas

characteristic: organ

text: Homo sapiens

characteristic: organism

text: Not 1

text: Sal 1

text: Both

text: Islets of Langerhans

characteristic: tissue-type

text: Melton Normalized Human Islet 4 N4-HIS 1

text: plasmid (ampicillin resistant)

text: pSPORTl


text: Zhejiang University

text: live

text: Generic

text: 10 days after anthesis

text: Oryza sativa

text: Endosperm

text: Endosperm library from Oryza sativa (10 days after anthesis)

characteristic: title


characteristic: External Id

text: Zhejiang University

text: live

text: Generic

text: 3-5 leaf stage

text: Oryza sativa

text: Stem

text: Stem library from Oryza sativa (3-5 leaf stage)


text: Zhejiang University

text: live

text: Generic

text: Mature stage

text: Oryza sativa

text: leaf

text: Oryza sativa mature leaf library induced by M.grisea

characteristic: vector


text: Univ. Saarland

characteristic: INSDC center name

text: live

text: Generic

text: brain

text: Homo sapiens

text: male

characteristic: sex

text: astrocytoma grade 2

text: Homo sapiens astrocytoma grade 2


text: Institute of Basic Medical Science, Chinese Academy of Medical Sciences

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Prepuberal

text: Mus musculus

text: Male

text: BALB/c

text: Testis

text: Mouse prepuberal testis cDNA library


text: live

characteristic: INSDC status

text: Generic

text: Prepuberal

text: Sus scrofa

text: Male

text: Chinese Changbai

characteristic: strain

text: Testis

text: Swine prepuberal testis cDNA library


text: Colorado State University

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Medium-bud blastema

text: Ambystoma mexicanum

text: Regenerating forelimb

text: Axolotl Lambda Zap Library


text: Beijing Institute for Cancer Research

text: live

text: Generic

characteristic: cell-line

text: garlic induced; isolated from Allitridi-treated BGC823 cells

characteristic: description

text: Homo sapiens

text: gastric cancer

text: Homo sapiens gastric cancer cell line BGC823


text: CNRS, INSERM, ULP

text: live

text: Generic

text: Oligo dT cDNA library constructed from RNA pooled from gastrula stage zebrafish embryos

text: gastrula stage embryos

characteristic: dev-stage

text: Danio rerio

text: EcoRI

text: XhoI

text: gastrula stage cDNA library

text: Lambda Zap


text: Southwest Hospital, The Third Military Medical University

text: live

text: Generic

text: umbilical vein endothelial cells

characteristic: cell type

text: Homo sapiens

text: Plasmid subtractive library of human umbilical vein endothelial cells (HUVEC) stimulated by lipopolysaccharide


text: Southern Illinois University at Carbondale

text: live

text: Generic

text: Fusarium solani f. sp. glycines (causative agent of sudden death syndrome of soybean) was grown in liquid culture and mycelia were harvested. Total RNA was used for cDNA synthesis using SMART PCR cDNA synthesis Kit (CLONTECH, CA) and cloned in pGEM vector. ESTs were sequenced using M13 universal forward or reverse primer.

text: Grown in liquid culture

text: Mycelium

characteristic: organ

text: Fusarium phaseoli

characteristic: organism

text: Fusarium solani f. sp. glycines EST Library

text: Plasmid

text: pGEM


text: Lawrence Livermore National Laboratory

text: live

text: Generic

text: 1st strand cDNA was primed with a NotI - oligo(dT) primer 5'-AACTGGAAGAATTCGCGGCCGCACGCGTTTTTTTTTTTTTTTTTTT-3'; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' AND 5'-CCTCGTGCCG-3'(Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library went through one round of normalization, and was constructed in the laboratory of M. Bento Soares (University of Iowa).

text: between 2 weeks - 2 months

text: DH10B (phage-resistant)

characteristic: lab-host

text: brain

text: Mus musculus

text: NotI

text: EcoRI

text: pituitary

characteristic: tissue-type

text: Soares NMAP

characteristic: title

text: phagemid (ampicillin resistant)


characteristic: External Id

text: Lawrence Livermore National Laboratory

characteristic: INSDC center name

text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.6 kb. Library constructed by Life Technologies.

text: 10 weeks

text: DH10B (T1-resistant)

text: kidney

text: Mus musculus

text: NotI

text: SalI

text: female

text: plasmid (ampicillin resistant)

text: pCMV-SPORT6 (Life Technologies)

characteristic: vector


text: Lawrence Livermore National Laboratory

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.1 kb. Library constructed by Life Technologies.

text: 5 months

text: DH10B (T1-resistant)

text: lung

text: Mus musculus

text: NotI

text: SalI

text: male

characteristic: sex

text: plasmid (ampicillin resistant)

text: pCMV-SPORT6 (Life Technologies)


text: Lawrence Livermore National Laboratory

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.63 kb. Library constructed by Life Technologies.

text: 5 months

text: DH10B (T1-resistant)

text: small intestine

text: Mus musculus

text: NotI

text: SalI

text: male

text: plasmid (ampicillin resistant)

text: pCMV-SPORT6 (Life Technologies)


text: A.I. Virtanen Institute for Molecular Sciences

text: live

text: Generic

characteristic: NCBI submission package

text: Adult

text: Brain

text: Rattus norvegicus

text: male

text: Wistar

characteristic: strain

text: Entorhinal cortex

text: Strategene lambda Zap converted to pBluescriptSK

text: Plasmid

text: pBluescriptSK


text: Northwestern University

text: live

text: Generic

text: I cloned a renal cell carcinoma relative EST sequence by using suppression subtraction hybridization (SSH) technique.

characteristic: description

text: kidney

text: Homo sapiens

text: renal cell carcinoma

text: Human renal cell carcinoma subtractive library


text: The Institute of Physical and Chemical Research (RIKEN)

text: live

text: Generic

text: thymic cells

characteristic: cell type

text: 2 days neonate

characteristic: dev-stage

text: Mus musculus

text: thymus

text: RIKEN full-length enriched, 2 days neonate thymus thymic cells


text: Clemson University

text: live

text: Generic

text: Morex

characteristic: cultivar

text: Plants were grown at the University of Minnesota in the GJ Muehlbauer lab; spikes were harvested and snap frozen at 0, 1, 2, 3, 4, 5, 6, and 8 days after Fusarium graminearum inoculation (Heinen). In the TJ Close lab at the University of California, Riverside, total RNA was prepared from each sample pool, equal quantities of all eight RNA pools were combined, poly(A) RNA was purified from the mixture, one primary unamplified cDNA library was made, and 1 million pfu were in vivo excised to give pBluescript SK(-) cDNA phagemids (Choi, Fenton, Malatrasi). Phagemids were plated and picked at the Clemson University Genomics Institute (CUGI) (Begum, Palmer, Frisch, Atkins and Wing). Plasmid DNA preparations, DNA sequencing and sequence analysis were performed at CUGI (Wing, Yu, Frisch, Henry, Simmons, Oates, Rambo, Main). The sequence has been trimmed to remove vector sequence and contains a minimum of 100 bases of phred value 20 or above. For more details on library preparation and sequence analysis see http://www.genome.clemson.edu/projects/barley. To order this clone see http://www.genome.clemson.edu/orders Also see Close TJ, Wing R, Kleinhofs A, Wise R (2001) Genetically and physically anchored EST resources for barley genomics. Barley Genetics Newsletter 31:29-30. (http://wheat.pw.usda.gov/ggpages/bgn/31/cover.html)

text: Hordeum vulgare subsp. vulgare

characteristic: organism

text: EcoRI

text: XhoI

text: Spike

text: Hordeum vulgare spike EST library HVcDNA0012 (Fusarium infected)

text: Phagemid

text: pBluescript SK(-)


text: Clemson University

text: live

text: Generic

text: Morex

text: Morex (mla) plants were greenhouse grown in the R Wise lab at Iowa State University, Ames, IA; 7 day old green seedlings were infected with isolate 5874 of Blumeria graminis f. sp. hordei, and leaves were harvested 24, 48 and 72 hr post-inoculation and snap frozen (Wise). In the TJ Close lab at the University of California, Riverside, total RNA was prepared from each sample pool, equal quantities of all three RNA pools were combined, poly(A) RNA was purified from the mixture, one primary unamplified cDNA library was made, and 1 million pfu were in vivo excised to give pBluescript SK(-) cDNA phagemids (Chin). Phagemids were plated and picked at the Clemson University Genomics Institute (CUGI) (Begum, Palmer, Frisch, Atkins and Wing). Plasmid DNA preparations, DNA sequencing and sequence analysis were performed at CUGI (Wing, Yu, Frisch, Henry, Simmons, Oates, Rambo, Main). The sequence has been trimmed to remove vector sequence and contains a minimum of 100 bases of phred value 20 or above. For more details on library preparation and sequence analysis see http://www.genome.clemson.edu/projects/barley. To order this clone see http://www.genome.clemson.edu/orders Also see Close TJ, Wing R, Kleinhofs A, Wise R (2001) Genetically and physically anchored EST resources for barley genomics. Barley Genetics Newsletter 31:29-30. (http://wheat.pw.usda.gov/ggpages/bgn/31/cover.html)

characteristic: lab-host

text: Hordeum vulgare subsp. vulgare

text: EcoRI

text: XhoI

text: green seedling leaf

characteristic: tissue-type

text: Hordeum vulgare green seedling EST library HVcDNA0014 (Blumeria infected)

text: Phagemid

text: pBluescript SK(-)


characteristic: External Id

text: Clemson University

text: live

text: Generic

text: Morex

text: Plants were grown at Washington State University, Pullman, WA in a greenhouse, the rachises were excised and frozen in liquid nitrogen (Kleinhofs lab). In the TJ Close lab at the University of California, Riverside total RNA was prepared, poly(A) was purified, one primary unamplified cDNA library was made, and 1 million pfu were in vivo excised to give pBluescript SK(-) cDNA phagemids (Chin). Phagemids were plated and picked at the Clemson University Genomics Institute (CUGI) (Begum, Palmer, Frisch, Atkins and Wing). Plasmid DNA preparations, DNA sequencing and sequence analysis were performed at CUGI (Wing, Yu, Frisch, Henry, Simmons, Rambo, Main). The sequence has been trimmed to remove vector sequence and contains a minimum of 100 bases of phred value 20 or above. For more details on library preparation and sequence analysis see http://www.genome.clemson.edu/projects/barley. To order this clone see http://www.genome.clemson.edu/orders Also see Close TJ, Wing R, Kleinhofs A, Wise R (2001) Genetically and physically anchored EST resources for barley genomics. Barley Genetics Newsletter 31:29-30. (http://wheat.pw.usda.gov/ggpages/bgn/31/cover.html)

text: Hordeum vulgare subsp. vulgare

text: EcoRI

text: XhoI

text: Rachis

text: Hordeum vulgare rachis EST library HVcDNA0015 (normal)

characteristic: title

text: Phagemid

text: pBluescript SK(-)


characteristic: INSDC center name

text: live

text: Generic

text: 14 days embryo

text: Mus musculus

text: eyeball

text: RIKEN full-length enriched, 14 days embryo eyeball


characteristic: INSDC first public

text: live

text: Generic

text: 8 cells

text: 8 cells embryo

text: Mus musculus

text: RIKEN full-length enriched, 8 cells embryo


characteristic: INSDC last update

text: live

text: Generic

text: 12 days embryo

text: Mus musculus

text: whole body

text: RIKEN full-length enriched, 12 days embryo whole body


text: live

text: Generic

text: 6 days neonate

text: Mus musculus

text: spleen

text: RIKEN full-length enriched, 6 days neonate spleen


text: Institute of Genetic Diagnosis, the Fourth Military Medical University

text: live

text: Generic

text: Osteosarcoma associated differentially expressed mRNA

text: Homo sapiens

text: DDRT-PCR (Li D)


text: NONG WOO BIO CO.,LTD.

text: live

text: Generic

text: The subtraction was performed using the PCR-select cDNA subtraction kit (Clontech, Palo Alto, CA) according to the manufacturer's instructions. For a tester, cDNA from HR-developing pepper leaves sampled after 3 days inoculation was synthesized using AMV reverse transcriptase while, for a driver, cDNA from noninoculated pepper leaves was synthesized. After the subtractive hybridization, the subtracted cDNAs are selectively amplified by using nested PCR primers to enrich differentially expressed sequences.

text: 7-8 weeks-old leaves

text: Capsicum chinense

text: EcoRI

text: hermaphrodite

characteristic: sex

text: leaf

text: PMMV-induced suppression subtractive cDNA library of Hot pepper

text: plasmid

text: pGEM-T easy

characteristic: vector


text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: pooled tissues ; (tissue_type=pituitary gland, dev_stage=adult, sex=male), (tissue_type=thymus, dev_stage=adult, sex=male), (tissue_type=forelimb, dev_stage=13 days embryo, sex=mixed), (tissue_type=testis, dev_stage=13 days embryo, sex=male), (tissue_type=olfactory brain, dev_stage=adult, sex=male)

text: Mus musculus

text: RIKEN full-length enriched, pooled tissues, pituitary, etc.


text: The Institute of Physical and Chemical Research (RIKEN)

text: live

text: Generic

characteristic: NCBI submission package

text: pooled tissues ; (tissue_type=cerebellum, dev_stage=16 days neonate, sex=mixed), (tissue_type=cerebellum, dev_stage=0 day neonate, sex=mixed), (tissue_type=hippocampus, dev_stage=adult, sex=male), (tissue_type=whole body, dev_stage=9 days embryo, sex=mixed), (tissue_type=lung, dev_stage=13 days embryo, sex=mixed)

text: Mus musculus

characteristic: strain

text: RIKEN full-length enriched, pooled tissues, cerebellum, etc.


text: live

text: Generic

text: melanocyte

characteristic: cell type

text: Mus musculus

text: RIKEN full-length enriched, melanocyte


text: live

text: Generic

text: B16 F10Y cells

text: Mus musculus

text: RIKEN full-length enriched, B16 F10Y cells


text: The Institute for Genomic Research (TIGR; www.tigr.org)

text: live

text: Generic

text: Kennebec

text: supplier: Cornell University, Fry lab; sequencing: The Institute for Genomic Research; Whole plants were challenged with 20,000 sporangia/ml of the compatible P. infestans isolate US 940480. Leaf tissue was collected at 3, 6, 9, 12, 24, 48, 72 hours after inoculation and frozen in liquid nitrogen immediately upon removal. Kennebec plants showed first symptoms of infection at 48 hours after inoculation. NOTE: We cannot exclude the possibility that this sequence is actually derived from Phytophthora rather than potato.

characteristic: description

text: 6 week old

characteristic: dev-stage

text: SOLR

text: Solanum tuberosum

text: EcoRI

text: XhoI

text: leaf

text: P. infestans-challenged potato leaf, compatible reaction

text: pBluescript SK(-)


text: live

text: Generic

text: Kennebec

characteristic: cultivar

text: supplier: Cornell University, Tanksley lab; sequencing; The Institute for Genomic Research. This library targets genes expressed in dormant tubers. This library was made from sections of dormant tuber, avoiding the buds and epidermis. Tubers were stored for one month post-harvest at 4oC. The tuber was peeled, well away from the surface. Then it was chopped into 1-2 mm cubes and immediately frozen in liquid nitrogen. This library is noted as P4 in Tanksley lab notebooks.

text: one month post-harvest

text: SOLR

text: Solanum tuberosum

characteristic: organism

text: EcoRI

text: XhoI

text: dormant tuber

text: potato tubers, dormant

text: pBluescript SK(-)


text: live

text: Generic

text: Bintje

text: Tissue supplied by Christian Bachem and Richard Visser (Department of Plant Breeding, Wageningen University, The Netherlands); sequencing by The Institute for Genomic Research. The cSTA libraries will attempt to capture the induction and initiation/initial growth of the tuber in an in vitro system as described in Bachem et al. (Plant Journal, 1996). Small microtubers develop from axillary buds attached to stem explants when placed on a high sucrose medium (10%). Visible morphological changes occur synchronously at day five in the axillary buds. The first library, cSTA (1-20) consists of axillary buds harvested on days 1-3. This targets those genes involved in induction of the microtubers. The following libraries, cSTA (21-40) and cSTA (41-60), capture genes involved in tuber initiation and outgrowth. This library is noted as P3 in Tanksley lab notebooks.

text: 7, 8 and 10 days

text: SOLR

characteristic: lab-host

text: Solanum tuberosum

text: EcoRI

text: XhoI

text: axillary buds of stem explants; growing sink-tubers

characteristic: tissue-type

text: potato microtubers, in vitro-grown

text: pBluescript SK(-)


characteristic: External Id

text: Clemson University

text: live

text: Generic

text: supplier: Boyce Thompson Institute; sequencing: The Institute for Genomic Research; cLEC - Cotyledons of seedlings 7-10 days post-germination were excised, cut at both ends and placed on MS medium with no selection. Mixed callus was harvested at 25 and 40 days and included undifferentiated masses. Tomato Callus EST Library

text: 25-40 days old

text: XL1-Blue MRF'

text: Solanum lycopersicum

text: EcoRI

text: XhoI

text: callus

text: tomato callus

characteristic: title

text: pBluescript SK(-)


text: Clemson University

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: supplier: Cornell University; sequencing: The Institute for Genomic Research; Flower buds and flowers were taken from greenhouse plants (4-8 wks old, TA496). They were immediately frozen in liquid nitrogen and then size-separated while remaining frozen.

text: 0-3mm buds

text: Solanum lycopersicum

text: EcoRI

text: XhoI

text: flower

text: tomato flower, buds 0-3 mm

text: pBluescript SK(-)


text: Clemson University

characteristic: INSDC last update

text: live

text: Generic

text: supplier: Cornell University; sequencing: The Institute for Genomic Research. Flower buds and flowers were taken from greenhouse plants (4-8 wks old, TA496). They were immediately frozen in liquid nitrogen and then size-separated while remaining frozen.

text: 3-8mm buds

text: Solanum lycopersicum

text: EcoRI

text: XhoI

text: flower

text: tomato flower, 3 - 8 mm buds

text: phagemid

text: pBluescript SK(-)


text: Clemson University

text: live

text: Generic

text: buds 8mm to preanthesis

text: Solanum lycopersicum

text: EcoRI

text: XhoI

text: flower

text: tomato flower, 8 mm to preanthesis buds

text: phagemid

text: pBluescript SK(-)

characteristic: vector


text: Clemson University

text: live

characteristic: INSDC status

text: Generic

text: anthesis

text: Solanum lycopersicum

text: EcoRI

text: XhoI

text: flower

text: tomato flower, anthesis

text: pBluescript SK(-)


text: Institute of Tropical Medicine

text: live

text: Generic

characteristic: NCBI submission model

text: hemocytes

text: Total RNA was isolated from hemocytes of uninfected and infected Biomphalaria glabrata (13-16-R1) snails and first strand cDNA was synthesized using anchored oligo-(dT)-primers (H-T11-A/C/G; GenHunter, Nashville, TN). 33P-labeled second strand synthesis was carried out using one of eight 13-mer arbitrary primers (GenHunter, Nashville, TN) in a PCR based amplification reaction. The labeled PCR products were separated using 6% polyacrylamide/8M urea gels and the differentially expressed fragments were identified by autoradiography. Bands of interest were sliced out from the gel and the DNA was extracted. This DNA was reamplified using the identical primer combination and SSCP analysis was performed. Differential expression of this fragment was not confirmed. However the PCR-product was cloned into the plasmid vector pCR4-TOPO (Invitrogen, Groningen, NL) and then subjected to sequencing. Insert was amplified using T3 and T7 vector specific primers.

text: adult

text: E.coli

text: Biomphalaria glabrata

text: hermaphrodite

characteristic: sex

text: hemocytes of not infected Biomphalaria glabrata 13-16-R1

text: Plasmid


text: Institute of Tropical Medicine

text: live

text: Generic

characteristic: NCBI submission package

text: hemocytes

text: adult

text: E.coli

text: Biomphalaria glabrata

text: hermaphrodite

characteristic: strain

text: hemocytes of infected (6-48h) Biomphalaria glabrata 13-16-R1

text: Plasmid


text: Research School of Biological Sciences, Australian National University

text: live

text: Generic

text: cDNA-harboring phagemids were obtained by performing mass excision procedure on honeybee brain cDNA lambda UniZap XR library (Ebert P.R. et al., 1998, Insect Mol. Biol. 7(2):151-62).

characteristic: description

text: adult worker

text: E.coli SOLR

text: brain

characteristic: organ

text: Apis mellifera

text: EcoRI

text: XhoI

text: female

text: central brain, mushroom bodies, central body, deuterocerebrum

text: Honeybee brain cDNA library

text: phagemid

text: pBluescript II SK-


text: Institute of Tropical Medicine

text: live

text: Generic

text: hemocytes

characteristic: cell type

text: Total RNA was isolated from hemocytes of uninfected and infected Biomphalaria glabrata (M-line) snails and first strand cDNA was synthesized using anchored oligo-(dT)-primers (H-T11-A/C/G; GenHunter, Nashville, TN). 33P-labeled second strand synthesis was carried out using one of eight 13-mer arbitrary primers (GenHunter, Nashville, TN) in a PCR based amplification reaction. The labeled PCR products were separated using 6% polyacrylamide/8M urea gels and the differentially expressed fragments were identified by autoradiography. The different bands of interest were sliced out from the gel and the DNA was extracted. This DNA was reamplified using the identical primer combination and differential expression was confirmed by SSCP. The PCR-product was cloned into the plasmid vector pCR4-TOPO (Invitrogen, Groningen, NL) and then subjected to sequencing. Insert was amplified using T3 and T7 vector specific primers.

text: adult

characteristic: dev-stage

text: E.coli

text: Biomphalaria glabrata

text: hermaphrodite

text: M-line

text: hemocytes of infected (6-48h) Biomphalaria glabrata M-line

text: Plasmid


text: Institute of Tropical Medicine

text: live

text: Generic

text: hemocytes

text: adult

text: E.coli

characteristic: lab-host

text: Biomphalaria glabrata

characteristic: organism

text: hermaphrodite

text: M-line

text: hemocytes of not infected Biomphalaria glabrata M-line

text: Plasmid


characteristic: External Id

text: Washington University School of Medicine

text: live

text: Generic

text: cDNA was synthesized from poly mRNA using an oligo-dT primer containing an XhoI site. Following second strand synthesis, EcoRI adapters were ligated to the cDNA and products were size- selected on sephacryl S500. The cDNAs were ligated to EcoRI/ XhoI prepared lambda ZapII (Stratagene). Clones were converted to phagemids by mass excision using ExAssist helper phage and E.coli SOLR cells (Stratagene). Insert sizes range from 0.3-3.0 kb. The library may contain a small percentage of host or bacterial contaminents. NOTE: Many clones lack the 5' EcoRI site but contain the upstream polylinker sites.

text: Tachyzoite

text: SOLR cells

text: Toxoplasma gondii

text: EcoRI

text: XhoI

text: VEG (Type III)

text: TgVEG-Tachyzoite cDNA

text: pBluescript SK-


text: live

text: Generic

text: PolyA mRNA from partially-sporulated oocysts was converted to cDNA using the template-switching PCR method (SMART cDNA, Clontech Inc.,) and sized selected on SizeSep 400 columns (Amersham Pharmacia Biotech Inc.). First strand was reverse transcribed using the CDS III-oligo-dT primer and a 5' template switch primer (Smart IV primer). The product of the first strand synthesis was PCR amplified using the same primer set and the fragments were digested with SfiI. The fragments were size selected, ligated into a modified pBluescript vector containing directional SfiI sites, and electroporated into DH10B or DH12S cells. Vector: SfiI sites were added to the multiple cloning region of pBluescript SK+ between the BamHI/EcoRI sites. The modified polylinker has the following sequence: 5'GAATTCGGCCATTACGGCC(G)n-- insert-- GGCCGCCTCGGCCCACGGATCC3'where n=3-4 G nucleotides. Library Source: Michael White, Maria E. Jerome,Emily A.Johnson, Jay A. Radke, Montana State University. Clone Availability: David Sibley, Washington University

text: Partially sporulated oocysts

text: Toxoplasma gondii

text: VEG (Type III)

text: TgVEG Partially sporulated oocyst cDNA

characteristic: title

text: Modified pBluescript (pBSSK+)


text: The University of Georgia, Department of Plant Biology

characteristic: INSDC center name

text: live

text: Generic

text: Isolated peripheral blood monocytes stimulated with E. coli lipopolysaccharide

text: The library was made from poly-A RNA in the cloning vector lambda ZAPII. Clones to be sequenced were prepared by mass excision.

text: Equus caballus

text: XhoI

text: EcoRI

text: Monocytes (MONO1)

text: Plasmid

text: pBluescript SK(-) from Lambda ZapII


text: University of Illinois

characteristic: INSDC first public

text: live

text: Generic

text: The library Gm-r1083 is a sequence-driven, reracked set of 4,992 clones selected from cDNA libraries from various tissues and stages of development of soybean. It represents 1117 sequences from the progenitor library Gm-c1009 (from mature roots of 2 month old greenhouse grown 'Williams' soybean plants); 820 sequences from the progenitor library Gm-c1013 (from 2 to 3 week old whole plants of Williams); and 3055 sequences from library Gm-c1028 (from 'Supernod' plants whose seedlings were innoculated with Bradyrhizobium japonicum, courtesy of Dr. Gary Stacey). The 5' ESTs of the source clones from the different progenitor libraries was used to select singletons, or a representative of each contig, which were reracked to form library Gm-r1083. The cDNA clones of the reracked Gm-r1083 library were then sequenced at the 3' end. The contig analysis to select unique genes was performed by the laboratory of Ernest Retzel, Center for Computational Genomics and Bioinformatics, University of Minnesota, http://web.ahc.umn.edu/biodata/nsfsoy/. Reracking was performed by Incyte Genomics, St. Louis, http://www.incyte.com, and 3' sequencing by the Keck Center for Comparative and Functional Genomics, University of Illinois, http://www.lie.uiuc.edu/biotech/keck.html. Note: The corresponding 5' EST from each clone in the Gm-r1083 library is listed in the 'OTHER EST' field. The detailed information on the source library for each clone can also be obtained by referring to the Incyte Genomics clone ID of the original cDNA library that is also listed under 'OTHER EST'.

text: Glycine max


text: Cardiff University

characteristic: INSDC last update

text: live

text: Generic

text: Tupaia belangeri

text: Retina

characteristic: tissue-type

text: Defocus-induced genes


text: Korea Advanced Institute of Science and Technology

text: live

characteristic: INSDC status

text: Generic

text: Kuwata

characteristic: cultivar

text: 26-30 days after pollination

text: Fruit

text: Cucumis melo

text: EcoRI

text: XhoI

text: Inodorus melon fruit cDNA library

text: Phage

text: Lamda ZapII

characteristic: vector


text: Station Biologique de Roscoff

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Old Blush

text: Mixed (young bud, open bud, mature flower, senescing flower)

text: Escherichia coli

text: Petal

text: Rosa chinensis

text: SfiI

text: SfiI

text: Hermaphrodite

characteristic: sex

text: Botanical garden, Lyon

text: Old Blush petal SMART library

text: Plasmid


text: Lawrence Livermore National Laboratory

text: live

text: Generic

text: Whole ovaries collected from zebrafish aged 4-5 months, 1 year and 2 years. Oligo-dT primed, directionally cloned. Average insert size 2 kb. Library constructed by Invitrogen and donated by R. Campbell (Marine Biology Laboratory, Woods Hole, MA).

characteristic: description

text: 4-5 months, 1 year and 2 years

text: DH10B (phage-resistant)

text: ovary(pooled)

characteristic: organ

text: Danio rerio

text: NotI

text: SalI

text: female

text: Campbell zebrafish ovary

text: phagemid (ampicillin resistant)


text: Stanford University

text: live

text: Generic

text: This library represents the unique genes found in the second round of EST sequencing at Stanford University for the maize genome project. Sequences are present from libraries 947 and 949. Contigs were assembled using ZmDBAssembler and 2 representatives from each contig were selected for the Unigene set. All singlets were also selected.

text: Zea mays

text: 1020 - Unigene II from Maize Genome Project


text: Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences; School of Basic Medicine, Peking Union Medical College

text: live

text: Generic

text: Lymphocyte

characteristic: cell type

text: 8 week

characteristic: dev-stage

text: Intestine

text: Mus musculus

text: EcoR I

text: EcoR I

text: male

text: BALB/c

characteristic: strain

text: epithelium

text: Subtracted Mouse Intestinal Intraepithelial Lymphocyte Library

text: Plasmid

text: pGEM-T Easy


characteristic: External Id

text: Lethbridge Research Center

text: live

text: Generic

text: Diapause specific cDNAs generated using suppression subtractive hybridization

text: Diapausing pupae

text: Haematobia irritans

characteristic: organism

text: EcoRI

text: XhoI

text: AAFC-LRC

text: Whole body

text: Horn Fly Diapause Lambda-ZAP Library

text: Phage

text: Lambda Zap


text: University of Texas at El Paso

characteristic: INSDC center name

text: live

text: Generic

text: Sequenced from RT-PCR products directly or T/A cloned into InVitrogen's pCR 2.1 vector and sequenced.

text: adult

text: liver

text: Fundulus heteroclitus

text: male

text: Fundulus heteroclitus liver

characteristic: title


text: University of Texas at El Paso

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: adult

text: liver

text: Fundulus heteroclitus

text: male

text: differential display products from Fundulus heteroclitus liver


text: Xiangya Medical College, Central South University

text: live

characteristic: INSDC status

text: Generic

text: Homo sapiens

text: multiple myeloma

characteristic: tissue-type

text: Homo sapiens multiple myeloma


text: Kyoto University

text: live

text: Generic

text: Branchiostoma belcheri

text: notochord

text: Amphioxus Notochord cDNA library


text: Universita di Milano

text: live

text: Generic

text: polymorphonuclear granulocytes

text: country=Italy

text: Bos taurus

text: Bos taurus polymorphonuclear granulocytes


text: Henan Normal University

text: live

text: Generic

text: Rattus norvegicus

text: Sprague-Dawley

text: liver

text: Rat liver


text: Institution of Medical Biology, CAMS

text: live

text: Generic

text: Fibroblast cDNA library induced by binding of IFN and poliovirus

text: Homo sapiens

text: lung

text: FIBIP


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: cDNA made by oligo-dT priming. Directionally cloned into EcoRI/XhoI sites using the following 5' adaptor: GGCACGAG(G). Library constructed by Ling Hong in the laboratory of Gerald M. Rubin (University of California, Berkeley) using ZAP-cDNA synthesis kit (Stratagene) and Superscript II RT (Life Technologies). Note: this is a NIH_MGC Library.

text: DH10B (phage-resistant)

characteristic: lab-host

text: pancreas

text: Homo sapiens

text: XhoI

text: EcoRI

text: ductal carcinoma, cell line

text: plasmid (chloramphenicol resistant, grown at 30C)

characteristic: vector


text: Instituto de Desarrollo Regional

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: C3 (corm enlargement)

text: Corm

text: Crocus sativus

text: Saffron Corm C3 Lambda Zap Express Library


text: University of Alabama

text: live

text: Generic

text: Spores were allowed to germinate for 4 days and then exposed to exudates of Ri T-DNA roots of carrot, for a 2-4 days at 32 C and 2% CO2 in liquid medium without a carbon source. RNA is extracted from germinated spores and used to prepare a cDNA library. ESTs are randomly selected and sequenced to identify differentially expressed genes induced by root exudates.

characteristic: description

text: Rhizophagus intraradices

text: Root-induced cDNA library from Glomus intraradices


text: Nanjing Medical University

text: live

text: Generic

text: adult worm

text: Schistosoma japonicum

text: Schistosoma japonicum, adult worm


text: Institut fur Pflanzenzuchtung und Pflanzenschutz

text: live

text: Generic

text: Mutin

characteristic: cultivar

text: RNA was isolated from plants 48 and 72 h post infection with Colletotrichum graminicola. After purification of mRNA first strand cDNA synthesis was performed, and infection-specific cDNAs were obtained after subtractive hybridization with photobiotinylated mRNA from vegetative hyphae and healthy maize leaves. The infection-specific cDNAs were subjected to second strand cDNA synthesis, and these cDNAs, representing putative differentially ESTs, were cloned into pCR 4-TOPO.

text: 48 to 72 hours post infection

text: E. colli Top 10

text: Zea mays/Colletotrichum graminicola mixed EST library

text: Infected leaves

text: Interaction-specific EST library

text: plasmid

text: pCR 4-TOPO (Topo TA cloning kit, Invitrogen)


text: Appalachian Fruit Research Station USDA-ARS

text: live

text: Generic

text: Suncrest

text: Prunus persica

text: green fruit

text: Prunus persica green fruit


text: Auburn University

text: live

text: Generic

text: Skin

characteristic: organ

text: Ictalurus punctatus

text: NotI

text: SalI

text: Skin cDNA library

text: Phagemid


text: North-western sci-tech university of agriculture and forestry

text: live

text: Generic

text: obtained by DDRT-PCR method in the persimmon maturation of Diospyros kaki L.f

text: fruit maturation

characteristic: dev-stage

text: Diospyros kaki

text: Diospyros kaki L.f

text: Persimmon differential expression


text: Univ. California Santa Barbara

text: live

text: Generic

text: adult

text: Mustela putorius furo

text: eye

text: Mustela putorius furo eye adult


text: Washington University School of Medicine

text: live

text: Generic

text: The library was constructed by Brandi Chiapelli and Dr. James McCarter at Washington University, St. Louis. The cDNA was made by using Dynabead oligo-dT priming (Dynal). PCR based library using a modified protocol from the SMART PCR cDNA Synthesis Kit from Clontech. Directionally cloned into the UDG sites of pAMP1. Dissected nematode tissues were provided by Dr. Alan Scott (ascott@jhsph.edu) of the School of Public Hygene and Public Health at John Hopkins University in Baltimore, MD.

text: Adult

text: Ascaris suum

text: NotI

text: SalI

text: Female

characteristic: sex

text: Dissected female gonad (DZ=differentiation zone, middle region of gonad)

text: Ascaris suum female gonad DZ pAMP1 v2 Chiapelli McCarter

text: plasmid (ampicillin resistant)

text: pAMP1 (Gibco)


characteristic: External Id

text: live

text: Generic

text: Adult

text: Ascaris suum

text: NotI

text: SalI

text: Female

text: Dissected female gonad (GZ=germinal zone, most distal region of gonad)

text: Ascaris suum female gonad GZ pAMP1 v2 Chiapelli McCarter

text: plasmid (ampicillin resistant)

text: pAMP1 (Gibco)


text: live

text: Generic

text: Adult

text: Ascaris suum

characteristic: organism

text: NotI

text: SalI

text: Female

text: Intestine

text: Ascaris suum female gut pAMP1 v2 Chiapelli McCarter

text: plasmid (ampicillin resistant)

text: pAMP1 (Gibco)


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

characteristic: INSDC center name

text: live

text: Generic

text: cDNA made by oligo-dT priming. Directionally cloned into EcoRI/XhoI sites using the following 5' adaptor: GGCACGAG(G). Library constructed by Ling Hong in the laboratory of Gerald M. Rubin (University of California, Berkeley) using ZAP-cDNA synthesis kit (Stratagene) and Superscript II RT (Life Technologies). Note: this is a NIH_MGC Library.

text: DH10B (phage-resistant)

text: kidney

text: Homo sapiens

text: XhoI

text: EcoRI

text: Wilms' tumor, cell line

text: plasmid (chloramphenicol resistant, grown at 30C)


text: live

text: Generic

text: DH10B (phage-resistant)

text: skin

text: Homo sapiens

text: XhoI

text: EcoRI

text: melanotic melanoma, cell line

characteristic: title


text: China Agricultural University

characteristic: INSDC first public

text: live

text: Generic

text: The EST sequences were gotten from differential display Xiang pig, Landrace and Large Yorkshire.

text: Longissimus Dorsi Muscle

text: Sus scrofa

text: Muscle

text: Swine Longissimus Dorsi Muscle cDNA Library


characteristic: INSDC last update

text: live

text: Generic

text: PolyA mRNA from fully-sporulated oocysts was converted to cDNA using the template-switching PCR method (SMART cDNA, Clontech Inc.,) and sized selected on SizeSep 400 columns (Amersham Pharmacia Biotech Inc.). First strand was reverse transcribed using the CDS III-oligo-dT primer and a 5' template switch primer (Smart IV primer). The product of the first strand synthesis was PCR amplified using the same primer set and the fragments were digested with SfiI. The fragments were size selected, ligated into a modified pBluescript vector containing directional SfiI sites, and electroporated into DH10B or DH12S cells. Vector: SfiI sites were added to the multiple cloning region of pBluescript SK+ between the BamHI/EcoRI sites. The modified polylinker has the following sequence: 5'GAATTCGGCCATTACGGCC(G)n-- insert-- GGCCGCCTCGGCCCACGGATCC3' where n=3-4 G nucleotides.

text: Fully sporulated oocysts

text: Toxoplasma gondii

text: BamHI

text: EcoRI

text: VEG (Type III)

characteristic: strain

text: TgVEG Fully sporulated oocyst cDNA

text: Modified pBluescript (pBSSK+)


text: Kyoto University

text: live

characteristic: INSDC status

text: Generic

text: young adult

text: Ciona intestinalis

text: whole animal

characteristic: tissue-type

text: Nori Satoh unpublished cDNA library, young adult


text: Kyoto University

text: live

text: Generic

text: cleaving embryo

text: Ciona intestinalis

text: whole animal

text: Nori Satoh unpublished cDNA library, cleavage stage embryo


text: Kyoto University

text: live

text: Generic

text: egg

text: Ciona intestinalis

text: whole animal

text: Nori Satoh unpublished cDNA library, egg


text: Kyoto University

text: live

text: Generic

text: larva

text: Ciona intestinalis

text: whole animal

text: Nori Satoh unpublished cDNA library, larva


text: University of Edinburgh

text: live

text: Generic

text: Teladorsagia circumcincta is a parasitic nematode. The library was constructed from mRNA from Teladorsagia circumcincta mixed adults.

text: adult

text: Teladorsagia circumcincta

text: BamHI

text: BamHI

text: mixed

text: Teladorsagia circumcincta adults library 2

text: phage

characteristic: vector


text: University of Innsbruck

text: live

text: Generic

characteristic: NCBI submission model

text: country=Austria

text: Gallus gallus

text: thyroid

text: Gallus gallus thyroid


text: University of Ghent & Vlaams Instituut voor Biotechnologie

text: live

text: Generic

characteristic: NCBI submission package

text: Sesbania rostrata

text: root primordia

text: Sesbania rostrata root primordia


text: Kyoto University

text: live

text: Generic

text: tailbud embryo

text: Ciona intestinalis

text: whole animal

text: Nori Satoh unpublished cDNA library, tailbud embryo


text: Korea Research Institute of Bioscience and Biotechnology

text: live

text: Generic

text: Bukang

text: 8 weeks after germination

text: Capsicum annuum

text: leaf inoculated with Xanthomonas campestris pv. glycines

text: pBluescript SK(-)


text: live

text: Generic

text: Hang Keun

text: 10 weeks after germination

text: Capsicum annuum

text: flower bud

text: Hybri-ZAP 2.1


text: live

text: Generic

text: Hang Keun

text: 10 weeks after germination

text: Capsicum annuum

text: anther

text: pBluescript SK(-)


text: China Agricultural University

text: live

text: Generic

text: The EST sequences were gotten from sequencing xiang pig muscle cDNA Library

characteristic: description

text: Longissimus Dorsi Muscle

text: Sus scrofa

text: Xiang Pig

text: Muscle

text: Swine Xiang Longissimus Dorsi Muscle cDNA Library


text: Iowa State University

text: live

text: Generic

characteristic: cultivar

text: Tissues: Germinated seed and seedlings (1, 2, 8, 11 DAG), Mixed mature tissures (17, 21, 38, 69, 77 DAG), Kernels (3, 5, 10, 15, 20, 25, 30, DAP), Adventious roots (65 DAG), Tassel (3-39 cm, 53 and 56 DAG), Immature ear (0.2-3.0 cm, 53, 56, 59 DAG), Husk (73 DAG), Silk, unpollinated first ear, ear shank, etiolated seedlings, callus, Cycloheximide-treated callus, Anaerobic treated seedlings, NAA (a-Naphthalene acetic acid)-treated seedlings, Kinetin-treated seedlings, ACPC (1-aminocyclopropane-1-carboxylix acid)-treated seedlings, Brassinolide-treated seedlings, ABA (Abscisic acid)-treated seedlings, GA (Gibberellic acid)-treated seedlings, JA (Jasmonic acid)-treated seedlings. ds-cDNA molecules were generated as follows. First-strand cDNA was prepared from oligo-dT selected mRNA by priming with a NotI oligo-dT primer (5' AACTGGAAGAATTCGCGGCCGCAGGAATTTTTTTTTTTTTTTTTT). The resulting DNA:RNA hybrid was treated with RNase H and used as a template for DNA PolI-catalyzed second strand synthesis. After the addition of EcoRI adaptors, the ds-cDNAs were digested with NotI and size-selected. The resulting molecules were directionally cloned into the EcoRI and NotI sites of the pT7T3PAC vector. The library then went through one round of normalization to CoT value of 5 based on the methods of Marcelo Bento Soares (Genome Research 6: 791-806, 1996).

characteristic: lab-host

text: Zea mays

text: EcoRI

text: NotI

text: mixed

text: Phagemid