characteristic: External Id

text: Agriculture and Agri-Food Canada

text: live

text: Generic

text: mRNA purified by Oligo(dT)-cellulose spun column using a mRNA purification kit (Amershan Pharmacia Biotech); cDNA library was constructed using the ZAP cDNA synthesis kit (Stratagene)

text: 3-day culture mycelium

text: Pieris brassicae derived isolate

characteristic: isolate

text: Zoophthora radicans

text: EcoR I

text: Xho I

text: Mycelium

text: ZRA

text: Plasmid

text: pBluescirpt SK-


status: PUBLIC

text: Agriculture and Agri-Food Canada

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

characteristic: description

text: 3-day culture mycelium

tag: attribute

characteristic: dev-stage

text: Plutella xylostella adapted isolate

text: Zoophthora radicans

characteristic: organism

text: EcoR I

text: Xho I

text: Mycelium

characteristic: tissue-type

text: ZRB

characteristic: title

text: Plasmid

text: pBluescirpt SK-

characteristic: vector


text: Madurai Kamaraj University

text: live

text: Generic

characteristic: cultivar

text: SSH library was made using 40 day old Solanum lycopersicum root as tester and 40 day old Solanum peruvianum root as driver to identify genes involved in lateral root development.

text: 40 day old plant

text: root

characteristic: organ

text: Solanum lycopersicum

text: bisexual

characteristic: sex

text: Solanum lycopersicum lateral root library

text: plasmid

text: pGEMT Easy


characteristic: External Id

text: Madurai Kamaraj University

text: live

text: Generic

text: SSH library was made using 8day old Solanum lycopersicum root as tester and Shoot as driver to identify root specific genes.

text: 8day old seedling

text: root

text: Solanum lycopersicum

text: bisexual

text: Solanum lycopersicum root specific library

text: plasmid

text: pGEMT Easy


text: Madurai Kamaraj University

text: live

text: Generic

text: Suppression Subtractive Hybridization library Roots of 40 - day old Solanum peruvianum Vs Roots of 40 - day old Solanum lycopersicum

text: Plant, 40 day old

text: Root

text: Solanum peruvianum

text: Bisexual

text: Solanum peruvianum Profuse Rooting Library

text: pGEM-T Easy


text: Madurai Kamaraj University

text: live

text: Generic

text: Suppression Subtractive Hybridization library Roots of 40 - day old plants Vs Roots of 8 - day old seedlings

text: Plant, 40 day old

text: Root

text: Solanum lycopersicum

text: Bisexual

text: Solanum lycopersicum Temporal Late Library

text: pGEM-T Easy


text: Madurai Kamaraj University

text: live

text: Generic

text: Suppression Subtractive Hybridization library Roots of 8 - day old seedlings Vs Roots of 40 - day old plants

text: Seedling - 8 day old

text: Root

text: Solanum lycopersicum

text: Bisexual

text: Solanum lycopersicum Temporal Early Library

text: pGEM-T Easy


text: Florida A & M University

text: live

text: Generic

text: Blanc du Bois

text: Used Subtractive hybridization to isolated mRNA induced specifically upon Xylella fastidiosa infestation from Xylem tissue.

text: Stem

text: Vitis hybrid cultivar

text: Xylem Tissue

text: Pierce's Disease induced genes (Blanc du Bois)


text: Florida A & M University

text: live

text: Generic

text: Zinfandel

text: Stem

text: Vitis vinifera

text: Xylem Tissue

text: Pierce's Disease induced genes (Zinfandel)


text: Justus-Liebig University of Giessen

text: live

text: Generic

text: subtracted cDNA library

text: adult

text: Tribolium castaneum

text: San Bernardino

characteristic: strain

text: whole animals, Immune challenged

text: Tribolium castaneum San Bernardino subtracted cDNA library

text: pGEM-T Easy


text: Medical College of Wisconsin

characteristic: INSDC center name

text: live

text: Generic

text: Chinchilla lanigera

text: H. flu infected middle ear mucosa

text: Kerschner - Ehrlich H. flu Infected Chinchilla Middle Ear Mucosa

text: plasmid

text: pCRII


text: USDA-ARS and University of Wisconsin

characteristic: INSDC first public

text: live

text: Generic

text: Straight 8

text: Cucumis sativus

text: Leaf

text: Cucumber cv. Straight 8 leaf cDNA

text: Plasmid


text: Purdue University

characteristic: INSDC last update

text: live

text: Generic

text: Composed of non blood fed and various blood fed and replete whole I. scapularis and I. scapularis life-cycle stages.

text: Ixodes scapularis

text: Eco RV

text: Not I

text: Wikel

text: Ixodes scapularis Pooled Non-infected Standard Library

text: Plasmid


text: Universidade Federal de Santa Catarina (UFSC)

text: live

characteristic: INSDC status

text: Generic

text: Total cloning of cDNA from T. solium larval stage (Cysticercus)

text: Larval (Cysticercus)

text: Sus scrofa

characteristic: lab-host

text: Taenia solium

text: EcoRI

text: EcoRI

text: Hermaphrodite

text: Cysti

text: pGEM-T-Easy


text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Total cloning of cDNA from T. solium larval stage (cysticercus) and possible Sus scrofa cDNA

text: Larval (Cysticercus)

text: Sus scrofa

text: Taenia solium

text: EcoRI

text: EcoRI

text: Hermaphrodite

text: Cysti-host

text: pGEM-T-Easy


text: University of Iowa Department of Biological Sciences

text: live

text: Generic

text: PCR-Select cDNA Subtraction Kit Clonetech

characteristic: description

text: stem gall

text: Solidago canadensis var. scabra

text: Solidago altissma enriched library for EST induced in plant gall initiated by Eurosta solidaginis

text: Invitrogen Topo-TA cloning kit


text: live

text: Generic

text: stem gall

text: Solidago canadensis var. scabra

characteristic: organism

text: Solidago altissma enriched library for EST down regulated in gall tissue initiated by Eurosta solidaginis

text: Invitrogen Topo-TA cloning kit


text: Universidade Federal do Amazonas

text: live

text: Generic

text: This sequences were obtained from fruits of camu camu, Myrciaria dubia and are part of the library CAMUEST, which is a library of cDNA developed for the INPA, Universidad Federal do Amazonas and EMBRAPA in Manaus and Brasilia in Brazil.

text: Myrciaria dubia

text: coat and fleshy tissue fruit

characteristic: tissue-type

text: CAMUEST


text: Northwest Fisheries Science Center

text: live

text: Generic

text: Reciprocal suppression subtractive hybridization cDNA libraries constructed between perinucleolus and mid-cortical alveolus stage ovarian follicles.

text: 10 and 22 month old fish with perinucleolus and mid-cortical alveolus stage follicles, respectively

characteristic: dev-stage

text: ovary

text: Oncorhynchus kisutch

text: female

text: whole ovarian tissue

text: Coho salmon perinucleolus and cortical alveolus stage suppression subtractive hybridization libraries

characteristic: title

text: Plasmid

text: TOPO pCR 2.1


text: Institute for Agricultural and Fisheries Research

text: live

text: Generic

text: Lolium perenne

text: Pistils

text: SI ryegrass library


text: Shanghai Fisheeries University

text: live

text: Generic

text: Pampus argenteus

text: skin and muscle

text: skin and muscle of Pampus argenteus Library

text: Plasmid

characteristic: vector


text: University of Ljubljana Faculty of Medicine

text: live

text: Generic

text: Mid-exponential growth phase

text: Hortaea werneckii

text: Hortaea werneckii MZKI-B736 SSH-MOS Sorbitol3M-1.5M Library


text: University of Sao Paulo - Faculty of Medicine

text: live

text: Generic

text: 8 days old adult

text: Apis mellifera

text: male

text: Africanized

text: mucus glands

text: Apis mellifera 8 days old adult RDA library on drone mucus gland


text: live

text: Generic

text: newly emerged adult

text: Apis mellifera

text: male

text: Africanized

text: mucus glands

text: Apis mellifera newly emerged adult RDA library on drone mucus gland


text: Huazhong Normal University

text: live

text: Generic

characteristic: cultivar

text: 1-14 day post anthesis

text: Gossypium hirsutum

text: EcoRI

text: XhoI

text: fiber/ovule

text: Cotton 1-14 day post anthesis Lambda Zap Express Library

text: plasmid

text: pBK-CMV


text: Chinese Academy of Sciences

text: live

text: Generic

text: Neopicrorhiza scrophulariiflora

text: rhizome

text: Subtracted rhizome-specific cDNA Library

text: Plasmid


text: NRC Institute for Marine Biosciences Industrial Partnership Facility

text: live

text: Generic

text: Gadus morhua

text: mixed digestive

text: Gadus morhua digestive tissue library


text: live

text: Generic

text: Gadus morhua

text: muscle

text: Gadus morhua skeletal muscle library


text: live

text: Generic

text: Gadus morhua

text: spleen

text: Gadus morhua spleen library (injected with polyriboinosinic polyribocytidylic acid)


text: live

text: Generic

text: Gadus morhua

text: head kidney

text: Gadus morhua head kidney SSH library enriched for genes up-regulated by polyriboinosinic polyribocytidylic acid (polyI:C)


text: Institut Pasteur

text: live

text: Generic

text: salivary gland sporozoite

characteristic: cell type

text: Plasmodium berghei

text: Plasmodium berghei NK65 salivary gland sporozoite


characteristic: External Id

text: live

text: Generic

text: Anopheles anthropophagus

text: female

characteristic: sex

text: SSH library from female Anopheles anthropophagus


text: Instituto Oswaldo Cruz

characteristic: INSDC center name

text: live

text: Generic

text: Santarem (Northern Brazil)

characteristic: isolate

text: Lutzomyia longipalpis

text: Lutzomyia longipalpis Fed 6h


text: Instituto Oswaldo Cruz

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Santarem (Northern Brazil)

text: Lutzomyia longipalpis

text: Lutzomyia longipalpis Fed 72h


text: Instituto Oswaldo Cruz

text: live

characteristic: INSDC status

text: Generic

text: Santarem (Northern Brazil)

text: Lutzomyia longipalpis

text: Lutzomyia longipalpis Infected


text: National Center for Cell Science

text: live

text: Generic

characteristic: NCBI submission model

text: A DSN (duplex specific nuclease)-based normalized cDNA library was prepared using Creator SMART cDNA Library Construction Kit (BD biosciences. Clontech, now Clontech Laboratories, Inc. A Takara Bio Company) according to the manufacturer's instructions.

text: Plasmodium yeolii-infected adult

text: midgut

characteristic: organ

text: Anopheles stephensi

text: Sfi I

text: Sfi I

text: female

text: NIV strain

characteristic: strain

text: midgut tissue

text: Plasmodium-infected mosquito midgut cDNA Library

text: Plasmid

text: pDNR-LIB


text: National Center for Cell Science

text: live

text: Generic

characteristic: NCBI submission package

characteristic: description

text: Adult

text: midgut

text: Anopheles stephensi

characteristic: organism

text: Sfi I

text: Sfi I

text: female

text: NIV strain

text: midgut tissue

text: Mosquito midgut cDNA Library

text: Plasmid

text: pDNR-LIB


text: University of Hawaii

text: live

text: Generic

text: SunUp

text: The library was constructed using ZAP-cDNA Synthesis kit (Stratagene, CA)

text: before meiosis stage

characteristic: dev-stage

text: E. coli

characteristic: lab-host

text: Flower

text: Carica papaya

text: EcoR I

text: Xho I

text: Female

text: flowers before meiosis stage

characteristic: tissue-type

characteristic: title

text: Plasmid

text: pBluescript SK-


characteristic: External Id

text: University of Hawaii

text: live

text: Generic

text: SunUp

characteristic: cultivar

text: before meiosis stage

text: E. coli

text: Flower

text: Carica papaya

text: EcoR I

text: Xho I

text: Hermaphrodite

text: flowers before meiosis stage

text: Plasmid

text: pBluescript SK-

characteristic: vector


text: University of Hawaii

characteristic: INSDC center name

text: live

text: Generic

text: SunUp

text: before meiosis stage

text: E. coli

text: Flower

text: Carica papaya

text: EcoR I

text: Xho I

text: Male

characteristic: sex

text: flowers before meiosis stage

text: Plasmid

text: pBluescript SK-


text: University of Hawaii

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: SunUp

text: after meiosis stage

text: E. coli

text: Flower

text: Carica papaya

text: EcoR I

text: Xho I

text: Female

text: flowers after meiosis stage

text: Plasmid

text: pBluescript SK-


text: University of Hawaii

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: SunUp

text: after meiosis stage

text: E. coli

text: Flower

text: Carica papaya

text: EcoR I

text: Xho I

text: Hermaphrodite

text: Hermaphrodite papaya flowers after meiosis stage

text: Plasmid

text: pBluescript SK-


text: SiChuan Agricultrual University

text: live

text: Generic

text: New Born

text: Leg Muscle

characteristic: organ

text: Gallus gallus

text: Differential Displayed EST in Four Kinds of Chicken's Leg Muscle

text: pBluescript-T


text: Nagoya University

text: live

text: Generic

characteristic: NCBI submission package

text: Chamaecyparis obtusa

text: differentiating xylem

text: Chamaecyparis obtusa Fluorescent differential display amplification products

text: pGEM-T Easy; TA-cloning


text: University of Toulouse / CNRS

text: live

text: Generic

text: SIx days old pregerminated spores of Gigaspora rosea were treated by acetone 0.001% (driver) or by GR24 10-8M (tester). After 1, 5, or 24h mRNA were extracted for carried out SSH library using SMART cDNA Library Construction Kit (Clontech).

characteristic: description

text: presymbiotic growth

text: Gigaspora rosea

characteristic: strain

text: Presymbiotic germinated spores and hyphae

text: Gigaspora rosea GR24 treated SSH library

text: cloning vector

text: pGEM-T easy (Promega)


text: Simon Fraser University

text: live

text: Generic

text: spermatozoa

characteristic: cell type

text: testis

text: Patiria miniata

characteristic: organism

text: male

text: Bat star testis


text: Simon Fraser University

text: live

text: Generic

text: oocytes

text: ovary

text: Patiria miniata

text: female

text: Bat star ovary


text: Dalian Fisheries University

text: live

text: Generic

text: adult

characteristic: dev-stage

text: Apostichopus japonicus

text: regenerating epidermis

characteristic: tissue-type

text: Sea cucumber 48/72 hours regenerating epidermis


characteristic: External Id

text: University of Illinois at Urbana-Champaign

text: live

text: Generic

text: Purified mRNA from antennae plus some labial palps was used to construct a plasmid library in Stratagene's host strain Epicurian Coli(r) XL-10 Gold(tm).

text: teneral adult

text: antenna

text: Rhagoletis suavis

text: Rhagoletis suavis antennal cDNA library

characteristic: title


text: Fundacion Ciencia para La Vida

text: live

text: Generic

text: dinoflagellate

text: The cDNA library was made using exponential growing Alexanrium catenella ACC07 strain free of associated bacteria, in normal culture conditions.

text: vegetative cells

text: Alexandrium catenella

text: NotI

text: EcoRV

text: undefined

text: whole organism

text: Alexandrium catenella ACC07 expressed sequence tags

text: plasmid

text: pExpress 1

characteristic: vector


text: Jilin University

characteristic: INSDC center name

text: live

text: Generic

text: Actinobacillus pleuropneumoniae

text: CVCC259 and CVCC263

text: Subtracted cDNA Libraries of Differencial Genes of Actinobacillus pleuropneumoniae serotype 1 and 5


text: COMAV-UPV

text: live

text: Generic

characteristic: cultivar

text: healthy

text: 15 days after pollination

text: Cucumis melo subsp. agrestis

text: root

text: Cucumis melo subsp. agrestis pat81 root 15 days after pollination healthy


text: COMAV-UPV

characteristic: INSDC first public

text: live

text: Generic

text: infected with Monosporascus cannonballus

text: 15 days after pollination

text: Cucumis melo subsp. agrestis

text: root

text: Cucumis melo subsp. agrestis pat81 root 15 days after pollination infected with M. cannonballus


text: COMAV-UPV

characteristic: INSDC last update

text: live

text: Generic

text: healthy

text: 46 days after pollination

text: Cucumis melo subsp. agrestis

text: root

text: Cucumis melo subsp. agrestis pat81 root 46 days after pollination healthy


text: COMAV-UPV

text: live

characteristic: INSDC status

text: Generic

text: 46 days after pollination

text: Cucumis melo subsp. agrestis

text: root

text: Cucumis melo subsp. agrestis pat81 root 46 days after pollination infected with M. cannonballus


text: COMAV-UPV

text: live

text: Generic

characteristic: NCBI submission model

text: healthy

text: Cucumis melo subsp. agrestis

text: root

text: Cucumis melo subsp. agrestis pat81 root healthy


text: COMAV-UPV

text: live

text: Generic

text: Cucumis melo subsp. agrestis

text: root

text: Cucumis melo subsp. agrestis pat81 root infected with M. cannonballus


text: COMAV-UPV

text: live

text: Generic

text: Cantaloupe C-35

text: infected with CMV

text: 15 days after pollination

text: Cucumis melo subsp. melo

text: cotyledon

text: Cucumis melo subsp. melo Cantaloupe C-35 cotyledon 15 days after pollination infected with CMV


text: COMAV-UPV

text: live

text: Generic

text: Cantaloupe C-35

text: infected with CMV

text: 46 days after pollination

text: Cucumis melo subsp. melo

text: cotyledon

text: Cucumis melo subsp. melo Cantaloupe C-35 cotyledon 46 days after pollination infected with CMV


text: COMAV-UPV

text: live

text: Generic

text: Cantaloupe C-35

text: infected with CMV

text: Cucumis melo subsp. melo

text: cotyledon

text: Cucumis melo subsp. melo Cantaloupe C-35 cotyledon infected with CMV


text: COMAV-UPV

text: live

text: Generic

text: Cantaloupe C-35

text: healthy

text: 15 days after pollination

text: Cucumis melo subsp. melo

text: leaf

text: Cucumis melo subsp. melo Cantaloupe C-35 leaf 15 days after pollination healthy


text: COMAV-UPV

text: live

text: Generic

text: Cantaloupe C-35

text: healthy

text: 46 days after pollination

text: Cucumis melo subsp. melo

text: leaf

text: Cucumis melo subsp. melo Cantaloupe C-35 leaf 46 days after pollination healthy


text: COMAV-UPV

text: live

text: Generic

text: Cantaloupe C-35

text: healthy

text: Cucumis melo subsp. melo

text: leaf

text: Cucumis melo subsp. melo Cantaloupe C-35 leaf healthy


text: COMAV-UPV

text: live

text: Generic

text: Piel de Sapo Pinyonet torpedo

text: healthy

text: 15 days after pollination

text: Cucumis melo subsp. melo

text: root

text: Cucumis melo subsp. melo Piel de Sapo Pinyonet torpedo root 15 days after pollination healthy


text: COMAV-UPV

text: live

text: Generic

text: Piel de Sapo Pinyonet torpedo

text: 15 days after pollination

text: Cucumis melo subsp. melo

text: root

text: Cucumis melo subsp. melo Piel de Sapo Pinyonet torpedo root 15 days after pollination infected with M. cannonballus


text: COMAV-UPV

text: live

text: Generic

text: Piel de Sapo Pinyonet torpedo

text: healthy

text: 46 days after pollination

text: Cucumis melo subsp. melo

text: root

text: Cucumis melo subsp. melo Piel de Sapo Pinyonet torpedo root 46 days after pollination healthy


text: COMAV-UPV

text: live

text: Generic

text: Piel de Sapo Pinyonet torpedo

text: 46 days after pollination

text: Cucumis melo subsp. melo

text: root

text: Cucumis melo subsp. melo Piel de Sapo Pinyonet torpedo root 46 days after pollination infected with M. cannonballus


text: COMAV-UPV

text: live

text: Generic

text: Piel de Sapo Pinyonet torpedo

text: healthy

text: Cucumis melo subsp. melo

text: root

text: Cucumis melo subsp. melo Piel de Sapo Pinyonet torpedo root healthy


text: COMAV-UPV

text: live

text: Generic

text: Piel de Sapo Pinyonet torpedo

text: infected with Monosporascus cannonballus

text: Cucumis melo subsp. melo

text: root

text: Cucumis melo subsp. melo Piel de Sapo Pinyonet torpedo root infected with M. cannonballus


text: COMAV-UPV

text: live

text: Generic

text: Piel de Sapo T-111

text: 15 days after pollination

text: Cucumis melo subsp. melo

text: fruit

text: Cucumis melo subsp. melo Piel de Sapo T-111 fruit 15 days after pollination


text: COMAV-UPV

text: live

text: Generic

text: Piel de Sapo T-111

text: 46 days after pollination

text: Cucumis melo subsp. melo

text: fruit

text: Cucumis melo subsp. melo Piel de Sapo T-111 fruit 46 days after pollination


text: National Research Institute of Aquaculture

text: live

text: Generic

text: embryos at shield stage

text: Paralichthys olivaceus

text: embryo

text: Paralichthys olivaceus ZIPLOX shield cDNA library


text: Fujian Agriculture and Forestry University

text: live

text: Generic

text: The tobacco suppression subtractive hybridization cDNA library was constructed from tobacco cultivar k326. The root were collected at vigorous growing stage, and placed into liquid N2 immediately and stored in -80oC freezer. Total RNA was isolated using TRNzol-Reagent (Tangen), mRNA was extracted from total RNA by mRNA extraction kit (promega). cDNA synthesis and differentially expressed RNA was prepared following the protocol of CLONTECH PCR-SelectTM cDNA Subtraction kit. Then the subtracted cDNA collection was used to construct library directly with pGEM-T-Easy vector. The library was constructed by Dr. Jianqing Pan and Dr. Weijian Zhuang.

text: vigorous growing stage

characteristic: lab-host

text: Roots

characteristic: organ

text: Nicotiana tabacum

text: plasmid

text: pGEM-T-Easy


text: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences

text: live

text: Generic

characteristic: NCBI submission package

text: Denglonghua

characteristic: description

text: bulb

text: Fritillaria cirrhosa

text: Fritillaria cirrhosa Bulbs cDNA Library

text: phagemid

text: pBluescriptII


text: Malaysia Genome Institute

text: live

text: Generic

text: Total RNA was extracted from Lates calcarifer brain tissue by the guanidium thiocyanate method. Following preparation of Lates calcarifer brain mRNA using the mRNA Purification Kit (Amersham Pharmacia Biotech), cDNA synthesis and library construction were carried out using the ZAP-cDNA Gigapack III Gold Cloning Kit (Stratagene). Lates calcarifer brain cDNAs cloned in the Lambda Uni-Zap XR vector were amplified once to produce a library with a titre of 1.16 X 105 pfu. Subsequently, Lambda Uni-Zap XR clones were in vivo excised into pBluescript phagemids for use as sequencing templates.

text: Juvenile

text: Brain

text: Lates calcarifer

text: EcoRI

text: XhoI

text: LBE

text: Phagemid

text: Lambda Uni-ZAP XR


text: live

text: Generic

text: 5 years old

text: ElectroMAX DH10B T1 Phage-Resistant Cells

text: Brain

text: Odocoileus virginianus

text: Sal I (5' end of cDNA)

text: Not I (3' end of cDNA)

text: Male

characteristic: sex

text: Brain stem

text: Deer Brain-stem Full-Length cDNA Library

text: pCMV SPORT 6.0


text: University of Alberta

text: live

text: Generic

text: 5 years old

text: Brain

text: Odocoileus virginianus

characteristic: organism

text: Sal I (5' end of cDNA)

text: Not I (3' end of cDNA)

text: Male

text: Cerebellum

text: Deer Cerebellum Full-Length cDNA Library

text: pCMV SPORT 6.0


text: University of Alberta

text: live

text: Generic

text: 5 years old

characteristic: dev-stage

text: Brain

text: Odocoileus virginianus

text: Sal I (5' end of cDNA)

text: Not I (3' end of cDNA)

text: Male

text: Thalamus

text: Deer Thalamus Full-Length cDNA Library

text: pCMV SPORT 6.0


text: University of Alberta

text: live

text: Generic

text: Standard full-length library

text: Spleen

text: Bos taurus

text: SalI

text: NotI

text: Spleen

characteristic: tissue-type

text: Bovine Spleen cDNA library

text: Phagemid

text: pCMV SPORT6


characteristic: External Id

text: Washington University School of Medicine

text: live

text: Generic

text: fifth instar stage (6-8 weeks post-fed fourths)

text: invitrogen ElectroMAX DH5a

text: Rhodnius prolixus

text: SfiIB

text: SfiIA

text: both

text: central nervous system tissue

text: Rhodnius_prolixus_EST_NADI_5th_instar_CNS

text: plasmid

text: pDNR-LIB


text: live

text: Generic

text: Total RNA isolated from sand fly. Library constructed by Express Genomics.

text: DH10B cells

text: Phlebotomus papatasi

text: NotI

text: EcorV

characteristic: title

text: plasmid


text: New Mexico State University

text: live

text: Generic

text: -1 DPA (days post-anthesis)

text: E. coli

text: Flower

text: Gossypium hirsutum

text: Anther

text: Gossypium hirsutum D8 restoration anther cDNA

text: pGEM-T

characteristic: vector


text: Centro de Pesquisas Rene Rachou - FIOCRUZ

characteristic: INSDC center name

text: live

text: Generic

text: Carcariae

text: Schistosoma mansoni

text: Mixed sex

text: LE

characteristic: strain

text: Plasmid

text: pBluscript


characteristic: INSDC first public

text: live

text: Generic

text: Solanum lycopersicum

text: leaf

text: tomato leave library


characteristic: INSDC last update

text: live

text: Generic

text: Carcariae

text: Schistosoma mansoni

text: Mixed sex

text: LE

text: Plasmid

text: pBluscript II


text: live

characteristic: INSDC status

text: Generic

text: Lung Stage (7 days of in vitro culture)

text: Schistosoma mansoni

text: Mixed sex

text: LE

text: Plasmid


text: live

text: Generic

characteristic: NCBI submission model

text: Schistosoma mansoni

text: Mixed sex

text: LE

text: OLD

text: Plasmid

text: pBluscript


text: live

text: Generic

text: Schistosoma mansoni

text: Mixed sex

text: LE

text: UKN

text: Plasmid

text: pBluscript


text: live

text: Generic

text: Egg

text: Schistosoma mansoni

text: Mixed sex

text: LE

text: Plasmid

text: pBluscript II


text: USDA-ARS; Jamie Whitten Delta States Research Center

text: live

text: Generic

text: A high quality EST with at least 200 contiguous bases at Trace Tuner score of 20 or better. Library construction by Earl Taliercio, USDA, ARS, Stoneville, MS, USA.

text: adult

text: Lygus lineolaris

text: female

text: whole body

text: Adult female tarnished plant bug


text: The Samuel Roberts Noble Foundation

text: live

text: Generic

text: Glandular-haired alfalfa plants were established in green house in 40 half gallon pots. Plants were grown in Metromix 350 and fertilized with MiracleGro as needed. They were cut back closely to encourage the emergence of vigorous shoots. Trichomes were isolated from stems, approximately 8-12 inches long, clipped from plants 2-3 inches above the crown. With minimal handling of the stem, the apical bud, leaves and nodes were discarded, and the trichomes isolated from the internode segments. The cDNA library from alfalfa trichome was constructed using the Creator Smart cDNA library construction kit (Clontech, Palo Alto, CA, USA) following the manufacturer's protocol for total RNA.

text: Trichome from the stem, approximately 8-12 inches long, clipped from plants 2-3 inches above the crown

text: Medicago sativa

text: Sfi IA (GGCCATTACGGCC)

text: Sfi IB (GGCCGCCTCGGCC)

text: Glandular trichomes isolated from stem

text: Alfalfa (Medicago sativa) Trichome isolated from the stem

text: pDNR-LIB


text: University of Stellenbosch

text: live

text: Generic

text: RNA extracted from 200mg Haliotis midae gill tissue with One Step RNA Reagent (Bio Basic Inc). 2ug mRNA isolated with polyA Spin mRNA Isolation Kit (New England Biolabs). First strand synthesis (from 2ug mRNA) primed with Oligo(dT) primer and library construction protocol followed using Universal RiboClone cDNA Synthesis System (Promega). cDNA inserts cloned into pGEM4-Z vector (Promega)

text: adult

text: JM109 (Promega)

characteristic: lab-host

text: gill

text: Haliotis midae

text: EcoRI

text: EcoRI

text: Haliotis midae gill cDNA

text: Plasmid

text: pGEM4-Z (Promega)


text: Scottish Crop Research Institute

text: live

text: Generic

text: Latham

characteristic: cultivar

text: Sequenced with M13 forward & reverse primers

text: Rubus idaeus subsp. strigosus

text: roots

text: Roots from Rubus strigosus


text: Northwest A & F University

text: live

text: Generic

characteristic: NCBI submission package

text: young leaves

characteristic: cell type

text: Total RNA was extracted by Biozol, reverse transcription was conducted by AMV reverse transcriptase; the double strand cDNA was synthesized and subjected to SSH analysis by using PCR Select cDNA Subtraction method Clontech company cDNA from the inoculated leaves used as the driver, cDNA from the control (no-inoculation) used as the tester, after twice hybridization the PCR products were purified and cloned into pGEM-T Easy vector and transformed to E coli strain DH5a, sequencing were conducted by M13+ primer.

text: leaf materials collected at three-leaf stage, after 3 days inoculation with the dominant race of powdery mildew pathogen in Guanzhong of Shaanxi province

text: Triticum aestivum

text: leaves

text: SSH-cDNA Library of no powdery mildew infection in leaves of powdery mildew resistant variety N9436 compared with infection


text: Northwest A & F University

text: live

text: Generic

text: young leaves

text: Total RNA was extracted by Biozol, reverse transcription was conducted by AMV reverse transcriptase; then double strand cDNA was synthesized and subjected to SSH analysis by using PCR Select cDNA Subtraction method Clontech company cDNA from the inoculated leaves used as the tester, cDNA from the control (no-inoculation) used as the driver, after twice hybridization the PCR products were purified and cloned into pGEM-T Easy vector and transformed to E coli strain DH5a, sequencing were conducted by M13+ primer.

characteristic: description

text: leaf materials collected at three-leaf stage, after 3 days inoculation with the dominant race of powdery mildew pathogen in Guanzhong of Shaanxi

text: Triticum aestivum

text: leaves

text: SSH-cDNA Library of powdery mildew infection in leaves of powdery mildew resistant variety N9436 compared with no infection


text: Northwest A & F University

text: live

text: Generic

text: young leaves

text: Triticum aestivum

characteristic: organism

text: leaves

text: SSH-cDNA Library of powdery mildew infection in leaves of powdery mildew resistant variety N9820 compared with no infection


text: University of Alberta

text: live

text: Generic

text: 5 years old

characteristic: dev-stage

text: ElectroMAX DH10B T1 Phage-Resistant Cells

text: Brain

characteristic: organ

text: Odocoileus virginianus

text: Sal I (5' end of cDNA)

text: Not I (3' end of cDNA)

text: Male

characteristic: sex

text: Brain stem

text: Deer Brainstem Full-Length cDNA Library

text: pCMV SPORT 6.0


characteristic: External Id

text: National Institute of Biomedical Innovation

text: live

text: Generic

text: 1st strand cDNA was primed with an oligo(dT) primer [ATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded cDNA was synthesized using specific 5' and 3' primers and amplified by PCR. The PCR product was digested with SfiI and size selection was performed to exclude fragments <1.5kb.The SfiI-digested PCR product was cloned into distinct DraIII sites of pME18S-FL3. XhoI sites just outside the DraIII sites can be used to isolate the cDNA insert. Libraries were constructed by oligo-capping method.

text: 11 years old

text: Pan troglodytes verus

text: DraIII (CACTGTGTG)

text: DraIII (CACCATGTG)

text: female

text: cerebellum cortex

characteristic: tissue-type

text: chimpanzee brain cDNA library PccB

characteristic: title

text: pME18S-FL3 (Acc.No. AB009864)


characteristic: INSDC center name

text: live

text: Generic

text: 11 years old

text: Pan troglodytes verus

text: DraIII (CACTGTGTG)

text: DraIII (CACCATGTG)

text: female

text: brain frontal lobe

text: chimpanzee brain cDNA library PflB

text: pME18S-FL3 (Acc.No. AB009864)

characteristic: vector


characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: 11 years old

text: Pan troglodytes verus

text: DraIII (CACTGTGTG)

text: DraIII (CACCATGTG)

text: female

text: brain occipital lobe

text: chimpanzee brain cDNA library PorA

text: pME18S-FL3 (Acc.No. AB009864)


text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: 11 years old

text: Pan troglodytes verus

text: DraIII (CACTGTGTG)

text: DraIII (CACCATGTG)

text: female

text: brain frontal lobe

text: chimpanzee brain cDNA library PflC

text: pME18S-FL3 (Acc.No. AB009864)


text: live

text: Generic

text: 11 years old

characteristic: lab-host

text: Pan troglodytes verus

text: DraIII (CACTGTGTG)

text: DraIII (CACCATGTG)

text: female

text: brain occipital lobe

text: chimpanzee brain cDNA library PorB

text: pME18S-FL3 (Acc.No. AB009864)


text: live

text: Generic

characteristic: NCBI submission package

text: 19 years old

text: Pan troglodytes verus

text: DraIII (CACTGTGTG)

text: DraIII (CACCATGTG)

text: male

text: skin, epidermal tissue

text: chimpanzee skin cDNA library PstA

text: pME18S-FL3 (Acc.No. AB009864)


text: live

text: Generic

text: 11 years old

text: Pan troglodytes verus

text: DraIII (CACTGTGTG)

text: DraIII (CACCATGTG)

text: female

text: liver

text: chimpanzee liver cDNA library PlvA

text: pME18S-FL3 (Acc.No. AB009864)


text: live

text: Generic

text: 11 years old

text: Pan troglodytes verus

text: DraIII (CACTGTGTG)

text: DraIII (CACCATGTG)

text: female

text: cerebellum cortex

text: chimpanzee brain cDNA library PccA

text: pME18S-FL3 (Acc.No. AB009864)


text: School of Forestry Northeast Forestry University

text: live

text: Generic

text: LEAF,YZSSH,YFSSH; Polygonum sibiricum were treated with NaHCO3

text: Knorringia sibirica

text: leaf

text: Polygonum sibiricum leaf

text: Plasmid

text: pGEM-T


text: live

text: Generic

text: JFSSH,JZSSH; Polygonum sibiricum were treated with NaHCO3

text: Knorringia sibirica

text: stem

text: Polygonum sibiricum stem

text: Plasmid

text: pGEM-T


text: Whitehead Institute for Biomedical Research

text: live

text: Generic

text: cDNA was generated from total RNA isolated from frozen chimpanzee testis tissue. cDNA was used for 454 sequencing.

text: Pan troglodytes

text: Male

text: Testis

text: Pan troglodytes Testis


text: University of California at Davis (UCD)

text: live

text: Generic

text: RACE-PCR was performed with the Marathon(TM) cDNA Amplification Kit (Clontech, Palo Alto, CA, USA) according to the manufacturer's instructions. Total RNA was extracted from the seven-day old seedling of Arabidopsis Col-0 plants using the TRIzol procedure (Invitrogen, Carlsbad, CA). Template mRNA was then purified from total RNA using Dynabeads Oligo (dT)25 (Dynal Biotech, Lake Success, NY, USA). RACE-PCR products were cloned into the pCR2.1-TOPO vector (Invitrogen, Carlsbad, CA) and sequenced.

text: seven-day old seedling

text: E.coli

text: Arabidopsis thaliana

characteristic: strain

text: Arabidopsis thaliana Col-0 RACE seven-day old seedling

text: plasmid

text: pCR2.1-TOPO vector


text: Konkuk University

text: live

text: Generic

text: The brainstem and liver cDNA libraries were constructed by Oligo-capping method. pCNS-D2 vector was used for ligation of double stranded cDNA obtained from the brainstem and liver libraries. Electro-competent DH10b cells were used for transformation.

characteristic: description

text: 5 days old

text: Sus scrofa

text: female

text: Korean native pig

text: brainstem

text: Full-length enriched cDNA from Korean native pig brainstem


text: Konkuk University

text: live

text: Generic

text: 5 days old

text: Sus scrofa

characteristic: organism

text: female

text: Korean native pig

text: liver

text: Full-length enriched cDNA from Korean native pig liver


text: Konkuk University

text: live

text: Generic

text: The spleen and neocortex libraries were constructed by using SMART kit (Clontech, Cat No. 634901). lambda Triplex 2 vector was used for cloning of double stranded cDNA from spleen and neocortex libraries. Electro-competent DH10b cells were used for transformation.

text: 5 days old

characteristic: dev-stage

text: Sus scrofa

text: female

text: Korean native pig

text: spleen

text: Full-length enriched cDNA from Korean native pig spleen


text: Konkuk University

text: live

text: Generic

text: 5 days old

text: Sus scrofa

text: female

text: Korean native pig

text: neocortex

text: Full-length enriched cDNA from Korean native pig neocortex


text: National Institute for Plant Genome Research

text: live

text: Generic

text: PRASAD

characteristic: cultivar

text: 21 days old seedlings

text: Setaria italica

text: cDNA-AFLP library of salt stressed foxtail millet


text: Oklahoma State University

text: live

text: Generic

text: E.coli

text: Bos taurus

text: Not I

text: Sal I

text: subcutaneous adipose tissue

text: cattle adipose tissue cDNA library

text: Plasmid

text: pSPORT 1


text: University of Florida/IFAS

text: live

text: Generic

text: adult

text: Culex nigripalpus

text: female

characteristic: sex

text: midgut

text: Mosquito midgut 12 h after females are fed a blood meal


characteristic: External Id

text: Universitaet Freiburg

text: live

text: Generic

text: An mRNA-Differential Display revealed sequences with higher expression level at P14 than at P0, identification through sequencing of the PCR product or plasmid DNA after blunt end cloning

text: postnatal day 14

text: inner ear

characteristic: organ

text: Rattus norvegicus

text: Wistar

text: organ of Corti

text: ESTs associated with the onset of hearing

text: Plasmid

text: pBluescript SK-


text: J. Craig Venter Institute (JCVI; www.jcvi.org)

text: live

text: Generic

text: Hawaii 4

text: Fragaria vesca

text: Salt stressed Fragaria vesca (strain Hawaii-4) cDNA library

characteristic: title


characteristic: INSDC center name

text: live

text: Generic

text: Hawaii 4

text: Fragaria vesca

text: Heat stressed Fragaria vesca (strain Hawaii-4) cDNA library


text: live

text: Generic

text: Hawaii 4

text: Fragaria vesca

text: Draught stressed Fragaria vesca (strain Hawaii-4) cDNA library


text: Centro de Pesquisas Rene Rachou - FIOCRUZ

text: live

text: Generic

text: Adult worm

text: Schistosoma mansoni

text: Mixed sex

text: LE

text: Plasmid

text: BA

characteristic: vector


characteristic: INSDC first public

text: live

text: Generic

text: Adult worm

text: Schistosoma mansoni

text: Mixed sex

text: LE

text: Plasmid


text: Life Sciences School, SOOCHOW University; Xinjiang Academy of Agricultural Sciences

characteristic: INSDC last update

text: live

text: Generic

text: These sequences obtained by SSH and the populations of gene fragments generated by subtractive hybridizations are differentially expressed in carnation.

text: Dianthus chinensis

text: Subtracted cDNA library of carnation between cold acclimation and normal ones


text: live

characteristic: INSDC status

text: Generic

text: These sequences obtained by SSH and the populations of gene fragments generated by subtractive hybridizations are differentially expressed in cotton.

text: Gossypium hirsutum

text: Subtracted cDNA library of cotton between cold acclimation and normal ones


text: Institute for Biochemistry, Faculty of Medicine

text: live

text: Generic

characteristic: NCBI submission model

text: Library was prepared using Clontec PCR-Selct cDNA Subtraction Kit (BD Bioscience) starting from total RNA from intact limb and reamputated limb at 24h. We produced tester cDNA population of intact tissue which we hybridysed with driver cDNA population of regenerated limbs. By doing this we harvested down-regulated genes in regenerating tissue. Subtracted library of down-regulated genes was cloned into pCRII plasmids using TA cloning kit (Invitrogen). All the obtained clones were sequenced.

text: One Shot Competent Cells

text: limb

text: Ambystoma mexicanum

text: male

text: wild type

text: Axolotl limb subtracted library, downregulated, 24h

text: Plasmid

text: pCRII


text: live

text: Generic

text: Library was prepared using Clontec PCR-Selct cDNA Subtraction Kit (BD Bioscience) starting from total RNA from intact limb and reamputated limb at 24h. We produced tester cDNA population of regenerated limbs which we hybridysed with driver cDNA population of intact tissue. By doing this we harvested up-regulated genes in regenerating tissue. Subtracted library of up-regulated genes was cloned into pCRII plasmids using TA cloning kit (Invitrogen). All the obtained clones were sequenced.

text: One Shot Competent Cells

characteristic: lab-host

text: limb

text: Ambystoma mexicanum

text: male

text: wild type

text: Axolotl limb subtracted library, upregulated, 24h

text: Plasmid

text: pCRII


text: live

text: Generic

characteristic: NCBI submission package

text: Library was prepared using Clontec PCR-Selct cDNA Subtraction Kit (BD Bioscience) starting from total RNA from intact limb and reamputated limb at 48h. We produced driver cDNA population of regenerated limbs which we hybridysed with tester cDNA population of intact tissue. By doing this we harvested down-regulated genes in regenerating tissue. Subtracted library of down-regulated genes was cloned into pCRII plasmids using TA cloning kit (Invitrogen). All the obtained clones were sequenced.

text: One Shot Competent Cells

text: limb

text: Ambystoma mexicanum

text: male

text: wild type

text: Axolotl limb subtracted library, downregulated, 48h

text: Plasmid

text: pCRII


text: live

text: Generic

text: Library was prepared using Clontec PCR-Selct cDNA Subtraction Kit (BD Bioscience) starting from total RNA from intact limb and reamputated limb at 48h. We produced tester cDNA population of regenerated limbs which we hybridysed with driver cDNA population of intact tissue. By doing this we harvested up-regulated genes in regenerating tissue. Subtracted library of up-regulated genes was cloned into pCRII plasmids using TA cloning kit (Invitrogen). All the obtained clones were sequenced.

text: One Shot Competent Cells

text: limb

text: Ambystoma mexicanum

text: male

text: wild type

characteristic: strain

text: Axolotl limb subtracted library, upregulated, 48h

text: Plasmid

text: pCRII


text: Universidade do Algarve, CCMAR_Center of Marine Sciences

text: live

text: Generic

text: Trophozoites

characteristic: cell type

text: ATCC 50984-ALG4

characteristic: cell-line

text: Perkinsus olseni

text: Perkinsus olseni ATCC 50984-ALG4


text: Instituto Potosino de Investigacion Cientifica y Tecnologica

text: live

text: Generic

text: Opuntia streptacantha

characteristic: organism

text: EcoRI

text: EcoRI

text: Opuntia streptacantha drought and heat Library

text: Plasmid

text: pCR4-TOPO (Invitrogen)


text: Institute of Himalayan Bioresource Technology

text: live

text: Generic

text: Leaf

text: Rheum australe

text: Leaf

characteristic: tissue-type

text: Rheum australe low-temperature inducible suppression subtractive hybridization (SSH) library

text: plasmid

text: pGEM-T Easy (Promega)


text: National Institute of Nutrition and Seafood Research

text: live

text: Generic

text: Directional cDNA library constructed from pooled liver samples from Atlantic cod (Gadus morhua) (Mean Weight 200 g) exposed to a mixture of organic and inorganic toxicants (PCB, PAH, Cu and Cd).

characteristic: description

text: Gadus morhua

text: liver

text: Gadus morhua pooled liver samples library (exposed to toxicants) GML

text: pBluescript II SK(+)


characteristic: External Id

text: live

text: Generic

text: Directional cDNA library constructed liver samples from mature wild Atlantic cod (Gadus morhua) (667 * 229 g, N = 3) caught in the Bergen harbour basin (Norway).

text: mature

characteristic: dev-stage

text: Gadus morhua

text: liver

text: Gadus morhua pooled liver samples library KL

text: pBluescript II SK(+)


text: live

text: Generic

text: Gadus morhua

text: Gadus morhua liver and intestine library TSTf

characteristic: title


characteristic: INSDC center name

text: live

text: Generic

text: intestine

characteristic: organ

text: Gadus morhua

text: Gadus morhua intestinal library ZNC

text: pBluescript II SK(+)


characteristic: INSDC first public

text: live

text: Generic

text: Directional cDNA library constructed from pooled gill samples from mature wild Atlantic cod (Gadus morhua) (667 * 229 g, N = 3) caught in Lungegardsvann (Bergen, Norway).

text: mature

text: Gadus morhua

text: gill

text: Gadus morhua pooled gill samples library ZNGAA

text: pBluescript II SK(+)

characteristic: vector


characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Directional cDNA library constructed from pooled kidney samples from mature wild Atlantic cod (Gadus morhua) (667 * 229 g, N = 3) caught in Lungegardsvann (Bergen, Norway).

text: mature

text: Gadus morhua

text: kidney

text: Gadus morhua pooled kidney samples library ZNKAA

text: pBluescript II SK(+)


text: live

text: Generic

characteristic: NCBI submission model

text: SSH cDNA library from pooled liver samples from Atlantic cod (Gadus morhua) (Mean Weight 200 g) exposed to a mixture of organic and inorganic toxicants (PCB, PAH, Cu and Cd) hybridized against a control group.

text: Gadus morhua

text: liver

text: Gadus morhua pooled liver samples SSH library (exposed to toxicants) ZNL

text: pBluescript II SK(+)


text: live

text: Generic

characteristic: NCBI submission package

text: Gadus morhua

text: Gadus morhua stem cell library ZNS

text: pBluescript II SK(+)


text: National Institutes of Health

text: live

text: Generic

text: Epithelial

text: PCR-based cDNA library made with SMART cDNA library construction kit from Clontech

text: Adults fed for 6 days

text: Midgut

text: Dermacentor variabilis

text: Female

characteristic: sex

text: Midgut

text: Adult Dermacentor variabilis 6 day fed midgut library (DVM)

text: Phage

text: Lambda TriplEx2


text: National Institutes of Health

text: live

text: Generic

text: Epithelial

characteristic: cell type

text: Adults unfed and fed for 2 days

text: Midgut

text: Dermacentor variabilis

text: Female

text: Midgut

text: Adult Dermacentor variabilis unfed/2 day fed midgut library (DVMUF/2DF)

text: Phage

text: Lambda TriplEx2


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 0.5-3kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. Library was constructed and sequencing done by the DOE Joint Genome Institute (Walnut Creek, CA).

text: Laccaria bicolor

text: SalI

text: NotI

text: CAHB Laccaria bicolor Mycelium interacted with signals from poplar roots (0.5-3k)


text: Washington University School of Medicine

text: live

text: Generic

text: adult

characteristic: lab-host

text: Heterorhabditis bacteriophora

text: BsrgI

text: BsrgI

text: plasmid


text: DOE Joint Genome Institute

text: live

text: Generic

text: Laccaria bicolor

characteristic: organism

text: SalI

text: NotI

text: CAHC Laccaria bicolor Vegetative mycelium and Fruiting body (0.5-3k)


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 3-10kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. Library was constructed and sequencing done by the DOE Joint Genome Institute (Walnut Creek, CA).

text: Laccaria bicolor

text: SalI

text: NotI

text: CAHF Laccaria bicolor Vegetative mycelium and Fruiting body (3-10k)


text: DOE Joint Genome Institute

text: live

text: Generic

text: Laccaria bicolor

text: SalI

text: NotI

text: CAHG Laccaria bicolor Vegetative mycelium and Fruiting body (3-10k)


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT)-primed cDNA and cloned into the p21_cDNA vector. PolyA RNA was primed with an oligo(dT) primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'-CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 3-10kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested p21_cDNA vector. Library was constructed and sequencing done by the DOE Joint Genome Institute (Walnut Creek, CA).

text: Laccaria bicolor

text: SalI

text: NotI

text: CAHH Laccaria bicolor Vegetative mycelium and Fruiting body (3-10k)


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: interacted with signals from poplar roots. The library was made from oligo(dT)-primed cDNA and cloned into the pMCL200_cDNA vector. PolyA RNA was primed with oligo(dT) primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'-CGGACGCGTGGG) and digested with NotI. cDNA was size selected >2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pMCL200_cDNA vector. Library was constructed and sequencing done by the DOE Joint Genome Institute (Walnut Creek, CA).

text: Laccaria bicolor

text: SalI

text: NotI

text: Mycelium

characteristic: tissue-type

text: CAPI Laccaria bicolor Mycelium interacted with signals from poplar roots H


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: interacted with signals from poplar roots. The library was made from oligo(dT)-primed cDNA and cloned into the pMCL200_cDNA vector. PolyA RNA was primed with oligo(dT) primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'-CGGACGCGTGGG) and digested with NotI. cDNA was size selected from >1.2-2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pMCL200_cDNA vector. Library was constructed and sequencing done by the DOE Joint Genome Institute (Walnut Creek, CA).

characteristic: description

text: Laccaria bicolor

text: SalI

text: NotI

text: Mycelium

text: CAPN Laccaria bicolor Mycelium interacted with signals from poplar roots M


characteristic: External Id

text: Instituto de Biociencias de Botucatu - IBB/UNESP

text: live

text: Generic

text: Creator(tm) SMART(tm) cDNA Library Construction kit (BD Biosciences)

text: adult

text: midgut

text: Anopheles aquasalis

text: Sfi IA

text: Sfi IB

text: male

text: midgut cDNA library from male Anopheles aquasalis

text: plasmid

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: Dark. The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected >2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed at DOE Joint Genome Institute (Walnut Creek, CA).

text: Vegetative mycelium 48h old

characteristic: dev-stage

text: DH10B T1 resist

text: Phycomyces blakesleeanus

text: SalI

text: NotI

text: minus

characteristic: strain

text: CBNA Phycomyces blakesleeanus NRRL1555 Vegetative mycelium 48h old Dark H


text: University of Liverpool

characteristic: INSDC center name

text: live

text: Generic

text: cDNA library prepared by Caroline

text: Adult

text: Brain

text: Cyprinus carpio

text: Caroline common carp adult brain

characteristic: title

text: Plasmid


characteristic: INSDC first public

text: live

text: Generic

text: L3 Activated

text: Lucigen E.cloni 10G Supreme

text: Ancylostoma caninum

text: HincII

text: HincII

text: Plasmid

text: pSMART


text: live

text: Generic

text: L3 Normal

text: Lucigen E.cloni 10G Supreme

text: Ancylostoma caninum

text: HincII

text: HincII

text: Plasmid

text: pSMART

characteristic: vector


text: University of Minnesota

characteristic: INSDC last update

text: live

text: Generic

text: heart

characteristic: organ

text: Meleagris gallopavo

text: skeletal muscle

text: Meleagris gallopavo embryo skeletal muscle

text: plasmid


text: DOE Joint Genome Institute

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: In Vivo Conditions: Dark. The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 0.6-1.2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed at DOE Joint Genome Institute (Walnut Creek, CA).

text: Vegetative mycelium 48h old

text: DH10B T1 resist

text: Phycomyces blakesleeanus

text: SalI

text: NotI

text: minus

text: CBNB Phycomyces blakesleeanus NRRL1555 Vegetative mycelium 48h old Dark L


text: DOE Joint Genome Institute

text: live

text: Generic

characteristic: NCBI submission package

text: In Vivo Conditions: Light. The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected >2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed at DOE Joint Genome Institute (Walnut Creek, CA).

text: Vegetative mycelium 48h old

text: DH10B T1 resist

text: Phycomyces blakesleeanus

text: SalI

text: NotI

text: minus

characteristic: sex

text: CBNC Phycomyces blakesleeanus NRRL1555 Vegetative mycelium 48h old Light H


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: Light. The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 0.6-1.2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed at DOE Joint Genome Institute (Walnut Creek, CA).

text: Vegetative mycelium 48h old

text: DH10B T1 resist

characteristic: lab-host

text: Phycomyces blakesleeanus

text: SalI

text: NotI

text: minus

text: CBNF Phycomyces blakesleeanus NRRL1555 Vegetative mycelium 48h old Light L


text: IASMA Research center

text: live

text: Generic

characteristic: cultivar

text: leaves

text: Malus domestica

characteristic: organism

text: Malus x domestica M.26 leaves cDNA-AFLP


text: IASMA Research center

text: live

text: Generic

text: leaves

text: Malus domestica

text: Malus x domestica G.41 leaves cDNA-AFLP


text: University of Liverpool

text: live

text: Generic

text: cDNA library prepared by Daryl

characteristic: description

text: Adult

text: Kidney

text: Cyprinus carpio

text: ECA

text: Plasmid


characteristic: External Id

text: University of Liverpool

text: live

text: Generic

text: cDNA library prepared by Daryl

text: Adult

text: Gill

text: Cyprinus carpio

text: ECB

text: Plasmid


text: University of Liverpool

text: live

text: Generic

text: cDNA library prepared by Daryl

text: Adult

characteristic: dev-stage

text: Spleen

text: Cyprinus carpio

text: ECC

text: Plasmid


text: University of Liverpool

text: live

text: Generic

text: cDNA library prepared by Daryl

text: Adult

text: Gill

text: Cyprinus carpio

text: WeyGill

text: Plasmid


text: Michigan State University

text: live

text: Generic

text: red delicious

text: Red Delicious transcripts from 6, 12, 24, 48 and 72 hours post-inoculation subtracted with Gala transcripts from 6, 12, 24, 48 and 72 hours post-inoculation

text: Malus domestica

text: Leaf

characteristic: tissue-type

text: Erwinia amylovora inoculated apple leaf cDNA suppression subtractive hybridization library


text: NRC Institute for Marine Biosciences

characteristic: INSDC center name

text: live

text: Generic

text: Osmerus mordax

text: Male

text: Liver

text: Rainbow smelt (Osmerus mordax) liver suppression subtractive libraries (fall and winter) combined

text: pCR 2.1-TOPO


text: Cold Spring Harbor Laboratory

text: live

text: Generic

text: Stratagene ZAP Express cDNA Synthesis Kit. The library was size-fractionated to enrich for large inserts. The library was then normalized to enrich for novel and rare cDNAs.

text: Ovules

text: Cycas rumphii

text: XhoI

text: Eco RI

text: Female

text: Cycas ovule - normalized (NYBG)

characteristic: title

text: Plasmid

text: pBK-CMV


text: Cold Spring Harbor Laboratory

characteristic: INSDC first public

text: live

text: Generic

text: Ovules

text: Ginkgo biloba

text: XhoI

text: Eco RI

text: female

text: Ginkgo ovule (NYBG)

text: Plasmid

text: pBK-CMV


text: Cold Spring Harbor Laboratory

text: live

text: Generic

text: Stratagene ZAP Express cDNA Synthesis Kit. The library was size-fractionated to enrich for large inserts.

text: Leaf

text: Gnetum gnemon

text: XhoI

text: Eco RI

text: female

text: Gnetum gnemon leaf (NYBG)

text: Plasmid

text: pBK-CMV

characteristic: vector


text: The University of Georgia

characteristic: INSDC last update

text: live

text: Generic

text: Normalized cDNA library was constructed from a single individual of Iris brevicaulis (IB72) collected in St. Martin Parish, Louisiana. Total RNAs from leaves and roots were isolated with Trizol (Invitrogen). cDNAs were synthesized and normalized with Trimmer-Direct cDNA Normalization Kit (Evrogen). The cDNAs >500 bp in length were used for cDNA library construction. cDNA library was constructed with Creator Smart cDNA Library Construction Kit (Clontech). The inserts were directionally cloned into the Clontech pDNR-LIB vector after Sfi I digestion. The plasmids were transformed to the host cell DH10B and selected by antibiotic chloramphenicol.

text: Iris brevicaulis

text: SfiI A (GGCCATTACGGCC)

text: SfiI B (GGCCGAGGCGGCC)

characteristic: strain

text: Roots and Leaves

text: Iris brevicaulis EST Library IB1

text: Plasmid

text: Clontech pDNR-LIB


text: The University of Georgia

text: live

characteristic: INSDC status

text: Generic

text: Normalized cDNA library was constructed from a single individual of Iris fulva (IF174) collected in Terrebonne Parish, Louisiana. Total RNAs from leaves and roots were isolated with Trizol (Invitrogen). cDNAs were synthesized and normalized with Trimmer-Direct cDNA Normalization Kit (Evrogen). The cDNAs >500 bp in length were used for cDNA library construction. cDNA library was constructed with Creator Smart cDNA Library Construction Kit (Clontech). The inserts were directionally cloned into the Clontech pDNR-LIB vector after Sfi I digestion. The plasmids were transformed to the host cell DH10B and selected by antibiotic chloramphenicol.

text: Iris fulva

text: SfiI A (GGCCATTACGGCC)

text: SfiI B (GGCCGAGGCGGCC)

text: Roots and Leaves

text: Iris fulva EST Library IF1

text: Plasmid

text: Clontech pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

characteristic: NCBI submission model

text: The library was made from oligo(dT)-primed cDNA and cloned into the pMCL200_cdna vector. PolyA RNA was primed with oligo(dT) primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'-CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 2-8kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pMCL200_cdna vector. Library was created and sequencing done by the DOE Joint Genome Institute (Walnut Creek, CA).

text: Artemisia annua

text: SalI

text: NotI

text: CAIZ Artemisia annua leaf


text: British Antarctic Survey

text: live

text: Generic

characteristic: NCBI submission package

text: adult

text: Harpagifer antarcticus

text: liver

text: Harpagifer antarcticus liver cDNA library


text: British Antarctic Survey

text: live

text: Generic

text: Harpagifer bispinis

text: liver

text: Harpagifer bispinis liver cDNA library


text: University of Natural Resources and Applied Life Sciences, Vienna

text: live

text: Generic

text: CM82036, Remus, 2 doubled-haploid lines CM82036*Remus

text: anthesis, 0-72 hours after Fusarium graminearum inoculation

text: Triticum aestivum

text: Fusarium graminearum inoculated floral tissue (palea, lemma, rachis)

text: wheat Fusarium graminearum inoculated, cDNA-AFLP fragment


text: University of California at Davis

text: live

text: Generic

text: seedling

text: Triphysaria versicolor

text: TA 158

text: root tip

text: T. versicolor host exudate forward SSH library

text: Gateway entry vector

text: pCR8/GW/TOPO TA


text: University of California at Davis

text: live

text: Generic

text: T. versicolor root tips were exposed in vitro to Arabidopsis root exudate for 0-5 hours at which time mRNA was isolated. Suppresssion subtractive hybridization was used to enrich for transcripts either up (forward) or down (reverse) regulated after exposure to treatment.

text: seedling

text: Triphysaria versicolor

text: TA 158

text: root tip

text: T. versicolor host exudate reverse SSH library

text: Gateway entry vector

text: pCR8/GW/TOPO TA


text: University of California at Davis

text: live

text: Generic

text: T. versicolor root tips were exposed in vitro to 100 uM peonidin for 0-5 hours at which time mRNA was isolated. Suppresssion subtractive hybridization was used to enrich for transcripts either up (forward) or down (reverse) regulated after exposure to treatment.

text: seedling

text: Triphysaria versicolor

text: TA 158

text: root tip

text: T. versicolor peonidin treated forward SSH library

text: Gateway entry vector

text: pCR8/GW/TOPO TA


text: University of California at Davis

text: live

text: Generic

text: seedling

text: Triphysaria versicolor

text: TA 158

text: root tip

text: T. versicolor peonidin treated reverse SSH library

text: Gateway entry vector

text: pCR8/GW/TOPO TA


text: University of California at Davis

text: live

text: Generic

text: RNA from T. versicolor root tips was hybridized against RNA from T. versicolor roots in a suppresssion subtractive hybridization reaction to enrich for tip specific transcripts.

text: seedling

text: Triphysaria versicolor

text: TA 158

text: root tip

text: T. versicolor root tip enriched SSH library

text: Gateway entry vector

text: pCR8/GW/TOPO TA


text: University of California at Davis

text: live

text: Generic

text: T. versicolor root tips were exposed in vitro to T. versicolor root extracts for 0-5 hours at which time mRNA was isolated. Suppresssion subtractive hybridization was used to enrich for transcripts either up (forward) or down (reverse) regulated after exposure to treatment.

text: seedling

text: Triphysaria versicolor

text: TA 158

text: root tip

text: T. versicolor self root extracts forward SSH library

text: Gateway entry vector

text: pCR8/GW/TOPO TA


text: University of California at Davis

text: live

text: Generic

text: seedling

text: Triphysaria versicolor

text: TA 158

text: root tip

text: T. versicolor self root extracts reverse SSH library

text: Gateway entry vector

text: pCR8/GW/TOPO TA


text: J. Craig Venter Institute (JCVI; www.jcvi.org)

text: live

text: Generic

text: Hawaii 4

text: Fragaria vesca

text: Salt and heat stressed Fragaria vesca (strain Hawaii-4) cDNA library


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: dark-phase. The library was made from oligo(dT)-primed cDNA and cloned into pBluescript SK- (Stratagene, La Jolla, CA). Library constructed at the NOAA Center for coastal Environmental Health and Biomolecular Research and sequencing performed at DOE Joint Genome Institute (Walnut Creek, CA).

text: log phase

text: Karenia brevis

text: Wilson, NOAA, USA

text: AUAO Karenia brevis Karbr Dark-phase


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: light+5 combined stress conditions. The library was made from oligo(dT)-primed cDNA and cloned into pBluescript SK- (Stratagene, La Jolla, CA). Library constructed at the NOAA Center for Coastal Environmental Health and Biomolecular Research and sequencing performed at DOE Joint Genome Institute (Walnut Creek, CA).

text: Karenia brevis

text: Wilson, NOAA, USA

text: CAGO Karenia brevis Karbr Stressed (light+5 combined stress conditions)


text: DOE Joint Genome Institite

text: live

text: Generic

text: The library was prepared from total RNA using the Creator SMART cDNA Library Construction Kit with the LD-PCR method to amplify the cDNA. Amplified cDNA was digested with SfiI, size selected for >400bp, and ligated into the pDNR-LIB vector.

text: 96 hours post fertilization

text: ElectroMAX DH10B

text: Acropora palmata

text: SfiI

text: SfiI

text: CAFB Acropora palmata 96 hours post fertilization

text: pDNR-LIB


text: DOE Joint Genome Institite

text: live

text: Generic

text: 15 day old larvae

text: ElectroMAX DH10B

text: Acropora palmata

text: SfiI

text: SfiI

text: CAFC Acropora palmata 15 day old larvae

text: pDNR-LIB


text: DOE Joint Genome Institite

text: live

text: Generic

text: The library was prepared from total RNA using the Creator SMART cDNA Library Construction Kit with the LD-PCR method to amplify the cDNA. Amplified cDNA was digested with SfiI, size selected for >400bp, and ligated into the pDNR-LIB vector. WARNING: this library contains a small percentage of cDNAs derived from the coral's symbiont, Symbiodinium strain KB8.

text: 15 day old larvae infected with Symbiodinium strain KB8

text: ElectroMAX DH10B

text: Acropora palmata

text: SfiI

text: SfiI

text: CAFF Acropora palmata 15 day old larvae infected with strain KB8

text: pDNR-LIB


text: DOE Joint Genome Institite

text: live

text: Generic

text: 96 hours post fertilization

text: ElectroMAX DH10B

text: Acropora palmata

text: SfiI

text: SfiI

text: CAOG Acropora palmata 96 hours post fertilization

text: pDNR-LIB


text: DOE Joint Genome Institite

text: live

text: Generic

text: 15 day old larvae

text: ElectroMAX DH10B

text: Acropora palmata

text: SfiI

text: SfiI

text: CAOH Acropora palmata 15 day old larvae

text: pDNR-LIB


text: DOE Joint Genome Institite

text: live

text: Generic

text: ElectroMAX DH10B

text: Acropora palmata

text: SfiI

text: SfiI

text: CAOI Acropora palmata 15 day old larvae infected with strain KB8

text: pDNR-LIB


text: DOE Joint Genome Institite

text: live

text: Generic

text: adult colony

text: ElectroMAX DH10B

text: Acropora palmata

text: SfiI

text: SfiI

text: CAWS Acropora palmata adult colony

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT)-primed cDNA and cloned into the p21_cDNA vector. PolyA RNA was primed with an oligo(dT) primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'-CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 1-2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested p21_cDNA vector. Library was created and sequencing done by the DOE Joint Genome Institute (Walnut Creek, CA).

text: Artemisia annua

text: SalI

text: NotI

text: leaf

text: CAIT Artemisia annua leaf


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT)-primed cDNA and cloned into the pMCL200_cdna vector. PolyA RNA was primed with oligo(dT) primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'-CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 0.6-2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pMCL200_cdna vector. Library was created and sequencing done by the DOE Joint Genome Institute (Walnut Creek, CA).

text: Artemisia annua

text: SalI

text: NotI

text: leaf

text: CAIU Artemisia annua leaf


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT)-primed cDNA and cloned into the pMCL200_cdna vector. PolyA RNA was primed with oligo(dT) primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'-CGGACGCGTGGG) and digested with NotI. cDNA was size selected using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pMCL200_cdna vector. Library was created and sequencing done by the DOE Joint Genome Institute (Walnut Creek, CA).

text: Artemisia annua

text: SalI

text: NotI

text: leaf

text: CAIW Artemisia annua leaf


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT)-primed cDNA and cloned into the pMCL200_cdna vector. PolyA RNA was primed with oligo(dT) primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'-CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 2-8kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pMCL200_cdna vector. Library was created and sequencing done by the DOE Joint Genome Institute (Walnut Creek, CA).

text: Artemisia annua

text: SalI

text: NotI

text: leaf

text: CAIX Artemisia annua leaf


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT)-primed cDNA and cloned into the pMCL200_cdna vector. PolyA RNA was primed with oligo(dT) primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'-CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 1.5-3kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pMCL200_cdna vector. Library was created and sequencing done by the DOE Joint Genome Institute (Walnut Creek, CA).

text: Artemisia annua

text: SalI

text: NotI

text: leaf

text: CAIY Artemisia annua leaf


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT)-primed cDNA and cloned into vector pDNR-LIB (Clontech Laboratories, Inc., Mountain View, CA) using the SMART system (BD Biosciences, San Jose, CA). To generate the first strand, polyA RNA was primed with an oligo(dT) primer (5'-ATTCTAGAGGCCGAGGCGGCCGACATG(T)30 VN-3', where V = A, G, or C). The second strand was synthesized by PCR using primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' followed by digestion of the SfiI sites. cDNA was size selected from 0.8-1.5kb using 1.1% agarose gel electrophoresis, then ligated into pDNR-LIB. Library was created by the Keasling Research Group, University of California, Berkeley, and sequencing done by the DOE Joint Genome Institute (Walnut Creek, CA).

text: Transition to flowering

text: Artemisia annua

text: SfiI A

text: SfiI B

text: glandular trichomes with 10-20% palisade cells and hair trichomes

text: CATA Artemisia annua, Tanzanian, from Sandeman Seeds, 0.8-1.5Kb

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT)-primed cDNA and cloned into vector pDNR-LIB (Clontech Laboratories, Inc., Mountain View, CA) using the SMART system (BD Biosciences, San Jose, CA). To generate the first strand, polyA RNA was primed with an oligo(dT) primer (5'-ATTCTAGAGGCCGAGGCGGCCGACATG(T)30 VN-3', where V = A, G, or C). The second strand was synthesized by PCR using primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' followed by digestion of the SfiI sites. cDNA was size selected from 1.5-2kb using 1.1% agarose gel electrophoresis, then ligated into pDNR-LIB. Library was created by the Keasling Research Group, University of California, Berkeley, and sequencing done by the DOE Joint Genome Institute (Walnut Creek, CA).

text: Transition to flowering

text: Artemisia annua

text: SfiI A

text: SfiI B

text: CATB Artemisia annua, Tanzanian, from Sandeman Seeds,1.5-2kb

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT)-primed cDNA and cloned into vector pDNR-LIB (Clontech Laboratories, Inc., Mountain View, CA) using the SMART system (BD Biosciences, San Jose, CA). To generate the first strand, polyA RNA was primed with an oligo(dT) primer (5'-ATTCTAGAGGCCGAGGCGGCCGACATG(T)30 VN-3', where V = A, G, or C). The second strand was synthesized by PCR using primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' followed by digestion of the SfiI sites. cDNA was size selected from 0.5-1kb using 1.1% agarose gel electrophoresis, then ligated into pDNR-LIB. Library was created by the Keasling Research Group, University of California, Berkeley, and sequencing done by the DOE Joint Genome Institute (Walnut Creek, CA).

text: Transition to flowering

text: Artemisia annua

text: SfiI A

text: SfiI B

text: CATC Artemisia annua, Tanzanian, from Sandeman Seeds, 0.5-1kb

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT)-primed cDNA and cloned into vector pDNR-LIB (Clontech Laboratories, Inc., Mountain View, CA) using the SMART system (BD Biosciences, San Jose, CA). To generate the first strand, polyA RNA was primed with an oligo(dT) primer (5'-ATTCTAGAGGCCGAGGCGGCCGACATG(T)30 VN-3', where V = A, G, or C). The second strand was synthesized by PCR using primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' followed by digestion of the SfiI sites. cDNA was size selected from 1-2kb using 1.1% agarose gel electrophoresis, then ligated into pDNR-LIB. Library was created by the Keasling Research Group, University of California, Berkeley, and sequencing done by the DOE Joint Genome Institute (Walnut Creek, CA).

text: Transition to flowering

text: Artemisia annua

text: SfiI A

text: SfiI B

text: CATF Artemisia annua, Tanzanian, from Sandeman Seeds, 1-2kb

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT)-primed cDNA and cloned into vector pDNR-LIB (Clontech Laboratories, Inc., Mountain View, CA) using the SMART system (BD Biosciences, San Jose, CA). To generate the first strand, polyA RNA was primed with an oligo(dT) primer (5'-ATTCTAGAGGCCGAGGCGGCCGACATG(T)30 VN-3', where V = A, G, or C). The second strand was synthesized by PCR using primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' followed by digestion of the SfiI sites. cDNA was size selected >2kb using 1.1% agarose gel electrophoresis, then ligated into pDNR-LIB. Library was created by the Keasling Research Group, University of California, Berkeley, and sequencing done by the DOE Joint Genome Institute (Walnut Creek, CA).

text: Transition to flowering

text: Artemisia annua

text: SfiI A

text: SfiI B

text: CATG Artemisia annua, Tanzanian, from Sandeman Seeds, 2+kb

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT)-primed cDNA and cloned into vector pAGEN1. Normalized library was created by the Keasling Research Group, University of California, Berkeley, and sequencing done by the DOE Joint Genome Institute (Walnut Creek, CA).

text: Transition to flowering

text: DH10B, T1 phage resistant

text: Artemisia annua

text: EcoRV

text: NotI

text: leaf

text: CAZI Artemisia annua normalized leaf library


text: USDA Agricultural Research Service

text: live

text: Generic

text: hemocyte

characteristic: cell type

text: Hemocytes were collected from immune-stimulated larvae. Total RNA was used to construct a directional non-normalized plasmid cDNA library in the vector pAGEN-1 by Agencourt Biosciences Corporation (Beverly, MA).

text: late fourth instar larvae

text: E. coli

text: Heliothis virescens

text: hemocyte

text: Heliothis virescens hemocyte Library

text: plasmid


text: Faculty of Veterinary Medicine, Ghent University

text: live

text: Generic

text: Sus scrofa

text: Male

characteristic: sex

text: Testis

text: Pig EST database


text: INRA - INSA de Lyon

text: live

text: Generic

text: Bacteriocyte

text: Subtracted Library (realised with PCR-selected cDNA substraction kit, Clontech laboratories). cDNA from E. Coli infected larvae and cDNA (SMART PCR cDNA synthesis kit,Clontech laboratories) from naive larvae were used as a tester and as a driver, respectively.

text: Fourth instar larvae

text: Bacteriome

characteristic: organ

text: Sitophilus zeamais

text: Lagoa (origin Azores)

text: Bacteriome

text: INF

text: Plasmid


text: Washington University School of Medicine

text: live

text: Generic

text: Normalization using Kamchakta Crab DNAse.

text: adult

characteristic: lab-host

text: Rhodnius prolixus

characteristic: organism

text: SfiIB

text: SfiIA

text: both

text: All tissue

text: plasmid

text: pDNR-LIB


characteristic: External Id

text: University of California at Davis

text: live

text: Generic

text: The library was made from a mixed pool of polyA-RNA isolated from T. pusilla roots after contact for 0-6 hrs with Arabidopsis roots, Arabidopsis root exudates, Triphysaria root extracts, DMBQ, peonidin or water. cDNAs were made using BD SMART technology (BD Biosciences) and normalized for transcript abundance by Duplex-Specific Nuclease Normalization using the kamchatka crab nuclease (Zhulidov et al., 2004). The library was constructed in the BD pDNR-LIB vector which allows simple transfer of sequenced insertions into Cre-Lox based acceptor vectors.

characteristic: description

text: Triphysaria pusilla

text: Triphysaria pusilla root tip normalized cDNA library


text: National Fisheries Research & Development Institute

text: live

text: Generic

text: brain

text: Sebastes schlegelii

text: Sebastes schlegeli brain cDNA library


text: live

text: Generic

text: intestine

text: Sebastes schlegelii

text: Sebastes schlegeli intestine cDNA library


text: live

text: Generic

text: liver

text: Sebastes schlegelii

text: Sebastes schlegeli liver cDNA library


text: live

text: Generic

text: spleen

text: Sebastes schlegelii

text: Sebastes schlegeli spleen cDNA library


text: AgResearch Ltd

text: live

text: Generic

text: A cDNA library was constructed in U39779 and sequenced using Sanger technology.

text: Microctonus hyperodae

text: Venom gland

characteristic: tissue-type

text: Microctonus hyperodae venom cDNAs

text: Plasmid

text: pTriplEx, U39779


text: DOE Joint Genome Institute

characteristic: INSDC center name

text: live

text: Generic

text: In Vivo Conditions: grown under different carbon sources in shaker or still culture conditions. The library was made from oligo(dT)-primed cDNA and cloned into the pMCL200_cdna vector. PolyA RNA was primed with oligo(dT) primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'-CGGACGCGTGGG) and digested with NotI. cDNA was size selected using 1.1% agarose gel electrophoresis (L ~0.6-2k, H ~>2k) then ligated into NotI and SalI-digested pMCL200_cdna vector. Library was created and sequencing done by the DOE Joint Genome Institute (Walnut Creek, CA).

text: 1 to 2 days

characteristic: dev-stage

text: Aspergillus niger

text: SalI

text: NotI

text: ATCC 1015

text: fungal biomass

text: CATZ Aspergillus niger fungal filamentous biomass grown under different carbon sources


characteristic: INSDC first public

text: live

text: Generic

text: mixed

text: Radopholus similis

text: SfiI

text: SfiI

characteristic: title

text: plasmid

text: pDNR-LIB


text: Peking University

characteristic: INSDC last update

text: live

text: Generic

characteristic: cultivar

text: Cotton Lambda Zap Express Library

text: -3-5 days post anthesis

text: Gossypium hirsutum

text: EcoRI

text: XhoI

text: ovule and fiber

text: Cotton ovule and fiber 3-5 days post anthesis

text: plasmid

text: pBK-CMV

characteristic: vector


text: University of California Santa Cruz

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Metatranscriptomic library from North Atlantic station SJ060903 (12.257 N 56.146 W) on 27-Jun-2006

text: uncultured marine microorganism


text: University of California Santa Cruz

text: live

text: Generic

characteristic: NCBI submission package

text: Metatranscriptomic library from North Atlantic station SJ060903 (12.257 N 56.146 W) on 27-Jun-2006 at 2200 hrs

text: uncultured marine microorganism


text: University of California Santa Cruz

text: live

text: Generic

text: Metatranscriptomic library from North Atlantic station SJ060912 (14.976 N 31.958 W) on 07-Jul-2006 at 1100 hrs

text: uncultured marine microorganism


text: University of California Santa Cruz

text: live

text: Generic

text: Metatranscriptomic library from North Atlantic station SJ060912 (14.976 N 31.958 W) on 07-Jul-2006 at 2030 hrs

text: uncultured marine microorganism


text: Ben Gurion University of the Negev

text: live

text: Generic

text: 48 hr embryo

text: Danio rerio


text: Emerging Technologies, QDPI&F

text: live

text: Generic

text: country: Australia:North Queensland; variety: hybrid 1B

text: Carica papaya

text: Female

characteristic: sex

text: fruit mesocarp and skin

text: Carica papaya fruit mesocarp and skin


text: Comav, Universidad Politecnica de Valencia

text: live

text: Generic

text: 3 days after Anthesis

text: Solanum lycopersicum

text: flower

text: SSH 3DPA


text: Agronomic Institute of Campinas

text: live

text: Generic

text: Citrus sinensis

text: leaf

text: Sweet orange leaf, greenhouse plant


text: Huaihai Institute of Technolegy

text: live

text: Generic

text: libraries were prepared using the SMART cDNA Library Construction Kit (Clontech)

text: Mactra antiquata

text: Creeping larvares

text: Coelomactra antiquata creeping larvares library


text: Baylor College of Medicine

text: live

text: Generic

text: CAP-trapper library constructed by Atsushi Nakabachi (RIKEN) with support of Nancy Moran (University of Arizona), Mizue Morioka (University of Tokyo), Hajime Ishikawa (University of the Air), and Takema Fukatsu (AIST). Pathogenic bacteria were removed from LSR1 clone by ampicillin treatment three months before RNA extraction.

text: mixed stage

text: Acyrthosiphon pisum

text: Sal I/Xho I

text: BamH I

text: Parthenogenetic female

characteristic: strain

text: Whole body, nymphs and adults, winged and wingless

text: Pea aphid whole body normalized full length cDNA library

text: Plasmid

text: pFLCIII


text: Hunan Agricultural University

text: live

text: Generic

text: ESTs related to fat deposit were obtained by using differential display RT-PCR

text: liver

characteristic: organ

text: Anser cygnoides

text: liver

text: Goose liver differential display RT-PCR


text: Hunan Agricultural University

text: live

text: Generic

text: Anser cygnoides

characteristic: organism

text: breast muscle

text: Goose breast muscular differential display RT-PCR


characteristic: External Id

text: Hunan Agricultural University

text: live

text: Generic

text: Anser cygnoides

text: abdomen fat

text: Goose abdomen fat differential display RT-PCR


text: Research Institute of Pomology and Floriculture

text: live

text: Generic

text: Ligol

text: cDNA-AFLP of apple response to putative systemic resistance inducers

characteristic: description

text: Malus domestica

text: leaf

text: Malus x domestica TDFs (Transcript Derived Fragments)

text: pGEM-T


text: Lund University

text: live

text: Generic

text: fibroblast

characteristic: cell type

text: Ornithorhynchus anatinus

text: Ornithorhynchus anatinus fibroblast Library


text: Zhejiang University

characteristic: INSDC center name

text: live

text: Generic

text: granulosa cells and oocytes

text: ovary

text: Ovis aries

text: Female

text: Ovis aries suppression substractive hybridization library


text: Sun Yat-sen University

text: live

text: Generic

text: Mikania micrantha

text: Mikania micrantha infected by Cuscuta campestris subtracted cDNA library1


text: State University of Campinas

text: live

text: Generic

text: somatic

text: Total RNA was extracted from the dikaryotic saprotrophic mycelium of M. perniciosa grown for 14 days on minimal media containing an exudate of liophilized cacao pod as the only carbon source and potassium nitrate as nitrogen source. Library was prepared from total RNA using the BD Creator TM SMART TM cDNA Library Construction kit (BD Biosciences-ClontechTM, USA, Palo Alto, CA), following the manufacturer's protocol, and cloned into the pDNR-LIB plasmid vector provided in the kit.

text: saprotrophic

characteristic: dev-stage

text: Theobroma cacao L.

characteristic: lab-host

text: Moniliophthora perniciosa

text: SfiI

text: SfiI

text: dikaryotic mycelium

characteristic: tissue-type

text: E-Sap-CP

text: Plasmid

text: pDNR-LIB (BD Biosciences-ClontechTM, USA, Palo Alto, CA)


text: State University of Campinas

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: somatic

text: Total RNA was extracted from the dikaryotic saprotrophic mycelium of M. perniciosa grown for 14 days on minimal media containing glucose as the only carbon source. Library was prepared from total RNA using the BD Creator TM SMART TM cDNA Library Construction kit (BD Biosciences-ClontechTM, USA, Palo Alto, CA), following the manufacturer's protocol, and cloned into the pDNR-LIB plasmid vector provided in the kit.

text: saprotrophic

text: Theobroma cacao L.

text: Moniliophthora perniciosa

text: SfiI

text: SfiI

text: dikaryotic mycelium

text: E-Sap-Glu

characteristic: title

text: Plasmid


text: State University of Campinas

text: live

characteristic: INSDC status

text: Generic

text: somatic

text: Total RNA was extracted from the monokaryotic biotrophic mycelium of M. perniciosa grown for 30 days on minimal media containing glycerol and a filtered extract of cacao meristems as carbon sources, and 0.5% yeast extract as nitrogen source. Library was prepared from total RNA using the BD Creator TM SMART TM cDNA Library Construction kit (BD Biosciences-ClontechTM, USA, Palo Alto, CA), following the manufacturer's protocol, and cloned into the pDNR-LIB plasmid vector provided in the kit.

text: biotrophic

text: Theobroma cacao L.

text: Moniliophthora perniciosa

text: SfiI

text: SfiI

text: monokaryotic mycelium

text: E-Bio-CM

text: Plasmid

characteristic: vector


text: State University of Campinas

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: somatic

text: Subtractive cDNA library. Total RNA was extracted from the dikaryotic saprotrophic mycelium of M. perniciosa grown for 14 days on minimal media containing either glucose (Glu) or cacao-pod extract (CP) as the only carbon source. For this subtracted library we generated two cDNA populations: CP (tester) and Glu (driver) using the Super SMART TM cDNA synthesis kit (BD Biosciences) and used in the subsequent steps of the suppressive subtractive hybridization procedure, according to the instructions of the BD Clontech TM PCR-Select TM Subtraction kit (BD Biosciences). The products of the secondary PCR were inserted into the pGEMÃ« TEasy plasmid vector (Promega Corporation, USA, Madison, WI) according to the manufacturer's instructions.

text: saprotrophic

text: Theobroma cacao L.

text: Moniliophthora perniciosa

text: dikaryotic mycelium

text: E-Sub(C-G)

text: Plasmid

text: pGEMTeasy (Promega)


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: Grown in Manganesis minus citiric acid production medium and glass baffled flask shaking culture. The library was made from oligo(dT)-primed cDNA and cloned into the pMCL200_cdna vector. PolyA RNA was primed with oligo(dT) primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'-CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 2-8kb using 1.1% agarose gel electrophoresis, then ligated into NotI and SalI-digested pMCL200_cdna vector. Library was created and sequencing done by the DOE Joint Genome Institute (Walnut Creek, CA).

text: day 1, 2, 3, 4, and 5

text: Aspergillus niger

text: SalI

text: NotI

text: ATCC 1015

characteristic: strain

text: pelleted fungal cells

text: CATW Aspergillus niger Pelleted fungal cells pooled from 1, 2, 3, 4, and 5 days cultures in citric acid production medium


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: Grown in Manganesis minus citiric acid production medium and glass baffled flask shaking culture. The library was made from oligo(dT)-primed cDNA and cloned into the pMCL200_cdna vector. PolyA RNA was primed with oligo(dT) primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'-CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 0.6-2kb using 1.1% agarose gel electrophoresis, then ligated into NotI and SalI-digested pMCL200_cdna vector. Library was created and sequencing done by the DOE Joint Genome Institute (Walnut Creek, CA).

text: day 1, 2, 3, 4, and 5

text: Aspergillus niger

text: SalI

text: NotI

text: ATCC 1015

text: pelleted fungal cells

text: CATX Aspergillus niger Pelleted fungal cells pooled from 1, 2, 3, 4, and 5 days cultures in citric acid production medium (2k)


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: grown under different carbon sources in shaker or still culture conditions. The library was made from oligo(dT)-primed cDNA and cloned into the pMCL200_cdna vector. PolyA RNA was primed with oligo(dT) primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'-CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 2-8kb using 1.1% agarose gel electrophoresis, then ligated into NotI and SalI-digested pMCL200_cdna vector. Library was created and sequencing done by the DOE Joint Genome Institute (Walnut Creek, CA).

text: 1 to 2 days

text: Aspergillus niger

text: SalI

text: NotI

text: ATCC 1015

text: fungal biomass

text: CATY Aspergillus niger fungal filamentous biomass grown under different carbon sources


text: Washington University

text: live

text: Generic

characteristic: cultivar

text: seeds, stems, leaves

text: Zea mays

text: pooled


text: College of Horticulture, Northwest A&F University

text: live

text: Generic

text: Thomson seedless

text: mixed stages 27-48d after bloom

text: E.coli XL1-Blue

text: ovule

characteristic: organ

text: Vitis vinifera

characteristic: organism

text: SfiI A and B

text: ovule cDNA library of a seedless grape (Vitis vinifera var.Thomson seedless)

text: Phage

text: Lambda TripIEX2


characteristic: External Id

text: Universidade Estadual de Londrina

text: live

text: Generic

text: P. brasiliensis differentially expressed cDNAs fragments cloned into pCR-Script or pGEM-TEasy vectors

characteristic: description

text: Paracoccidioides brasiliensis

text: P. brasiliensis Pb01 differentially expressed sequences


text: Universite de Poitiers

characteristic: INSDC center name

text: live

text: Generic

text: dulce

text: A forward substractive library was constructed (PCR-SelectTM substraction kit, BDR) from RNA extracted from phloem tissue of control and NaCl stressed plants.

text: 4 month old plant, 3 week salt (NaCl 300 mM) stress

text: Petiole

text: Apium graveolens

text: hermaphrodite

characteristic: sex

text: Phloem

text: Celery phloem library from NaCl treated plants

text: plasmid

text: pGEM T-EasyTM


text: Shiraz University

text: live

text: Generic

text: Seedlings of Shahi, a cold tolerant Iranian wheat were grown in growth chamber for 14 days. Isolation was carried out from leaves and by specific primers via PCR.

characteristic: dev-stage

text: Leaf

text: Triticum aestivum

text: Shahi

text: Triticum aestivum Shahi 14 days seedlings under normal condition


text: Universite Pierre et Marie Curie-Paris 6

text: live

text: Generic

text: embryo

text: Helianthus annuus

text: seed

characteristic: tissue-type

text: Helianthus annuus probe cloning


text: Marine Biology

characteristic: INSDC first public

text: live

text: Generic

text: This library has been constructed using PCR-Select cDNA Subtraction Kit (Clontech, CA, USA).

text: Escherichia coli (JM109)

characteristic: lab-host

text: Ulva fasciata

text: SSH cDNA Library of Ulva fasciata treated with 50 micro-M CuSO4 for 6, 9, 12h

text: plasmid

text: pGEM-T-easy vector


text: University of Massachusetts Medical School

characteristic: INSDC last update

text: live

text: Generic

text: The library was constructed and normalized as described by Bonaldo, M.F., Lennon, G. and Soares, M.B. (1996), Genome Research 6(9): 791-806. Previously sequenced SB02 clones were subtracted from the library. An identiying tag was added at the 3' during cDNA synthesis: insertAAAAAAAAAAAAAAAAAATGCGA.

text: Brain

text: Danaus plexippus

text: EcoRI(5' side of insert)

text: NotI (3' side of insert)

text: Brain

text: Normalized subtracted keck library BF01

characteristic: title

text: Plasmid

text: pBS II SK(+)


text: Sun Yat-sen University

text: live

characteristic: INSDC status

text: Generic

text: Mikania micrantha

text: Mikania micrantha infected by Cuscuta campestris 7 days subtracted cDNA library2


text: University of Washington

text: live

text: Generic

characteristic: NCBI submission model

text: Poly A RNA was prepared from total RNA prep. cDNA was size-selected, was NOT normalized, and cloned in lambda ZAP II EcoRI and XhoI-digested arms as per the kit manufacturer's protocol (Stratagene). Sequencing was carried out on pBluescript SK- phagemid templates selected by blue/white screening.

text: placenta

text: Papio anubis

text: EcoRI

text: XhoI

text: female

text: bacteriophage

text: lambda ZAP II

characteristic: vector


text: Tottori University

text: live

text: Generic

characteristic: NCBI submission package

text: plerocercoid

text: Spirometra erinaceieuropaei

text: Spirometra erinaceieuropaei plerocercoid


text: ARC Seibersdorf research GmbH

text: live

text: Generic

text: Yungay

characteristic: cultivar

text: 4 days post spray inoculation with Phytophthora infestans

text: adult

text: Solanum tuberosum

text: leaf

text: Solanum tuberosum cv. Yungay leaf adult


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT)-primed cDNA and cloned into the EcoRV (5') and NotI (3') sites of pBluescript SK- (Stratagene, La Jolla, CA). Library constructed at Williams College (Williamstown, MA) and sequencing was done at DOE Joint Genome Institute (Walnut Creek, CA).

text: Embryo st. 1-6

text: Helobdella robusta

text: EcoRV

text: NotI

text: CAWX Helobdella robusta Primary Early Library, Embryo st. 1-6

text: pExpress


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT)-primed cDNA and cloned into the EcoRV (5') and NotI (3') sites of pBluescript SK- (Stratagene, La Jolla, CA). Library is subtracted, and was constructed at Williams College (Williamstown, MA). Sequencing was done at DOE Joint Genome Institute (Walnut Creek, CA).

text: Embryo st. 1-6

text: Helobdella robusta

text: EcoRV

text: NotI

text: CAWY Helobdella robusta Subtracted Early Library, Embryo st. 1-6

text: pExpress


text: DOE Joint Genome Institute

text: live

text: Generic

text: Embryo st. 7-11

text: Helobdella robusta

text: EcoRV

text: NotI

text: CAWZ Helobdella robusta Primary Late Library, Embryo st. 7-11

text: pExpress


text: DOE Joint Genome Institute

text: live

text: Generic

text: Embryo st. 7-11

text: Helobdella robusta

text: EcoRV

text: NotI

text: CAXA Helobdella robusta Subtracted Late Library, Embryo st. 7-11

text: pExpress


text: Seoul National University

text: live

text: Generic

text: Aplysia kurodai

text: buccal muscle

text: Aplysia kurodai buccal muscle EST library

text: Plasmid

text: Uni-ZAP XR


text: Seoul National University

text: live

text: Generic

text: Aplysia kurodai

text: nervous system

text: Aplysia kurodai nervous system 5' EST library

text: Plasmid


text: Guru Gobind Singh Indraprastha University

text: live

text: Generic

text: The cDNA library was constructed from polyA+ RNA by using the Clontech SMART cDNA synthesis kit. The cDNAs were cloned directionally in pDNR-LIB vector.

text: Adult

text: Hippophae rhamnoides

text: Sfi IA

text: Sfi IB

text: Female

text: Leaf

text: Hippophae leaf library

text: Plasmid

text: pDNR-LIB


text: Seoul National University

text: live

text: Generic

text: Aplysia kurodai

text: nervous system

text: 5-HT treated Aplysia kurodai nervous system EST library

text: Plasmid

text: Uni-ZAP XR


text: Seoul National University

text: live

text: Generic

text: Aplysia kurodai

text: nervous system

text: Aplysia kurodai nervous system full-length enriched EST library

text: Plasmid


text: Seoul National University

text: live

text: Generic

text: Aplysia kurodai

text: nervous system

text: Aplysia kurodai nervous system random primed library

text: Plasmid


text: Huazhong Agricultural University

text: live

text: Generic

text: Citrus trifoliata

text: Poncirus trifoliata Suppression Subtractive Hybridization Library


text: University of Wisconsin-Milwaukee

text: live

text: Generic

text: Library constructed from RNA obtained from the gills of adult quagga mussels exposed for 8 weeks at a site off Atwater beach, Lake Michigan.

text: Dreissena rostriformis bugensis

text: pooled gills

text: Atwater quagga mussel gills

text: pBK-CMV