text: Agriculture and Agri-food Canada

text: live

text: Generic

text: mycelium in compatible wheat (Triticum aestivum) cultivar Thatcher

characteristic: cell type

text: mRNA obtained by grinding infected leaf tissue & polyA selection; first-strand synthesis by anchored polyT primer set (Wang et al., 2000, Proc Natl Acad Sci U S A, 97, 4162-7) followed by polyG tailing, PCR amplification, restriction with NotI plus Apa1, size-selection and directional cloning in pBlueScriptIIKS. The NotI site represents the 3'-end of the mRNA. PHRED20 sequence clipped for vector sequences. Some clones might have chimeric inserts linked through Apa1 or NotI sites.

text: haustorial, 6 days after inoculation

text: E. coli DH10B/r

characteristic: lab-host

text: Puccinia triticina

text: NotI

text: race 1 (design. BBB; Long et al., 1989, Plant Disease 73, 294)

text: Plasmid

text: pBSII(KS)


status: PUBLIC

characteristic: External Id

text: Agriculture and Agri-food Canada

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: germinated urediniospores stripped of epidermis of compatible wheat (Triticum aestivum) cultivar Thatcher

tag: attribute

text: fungal structures were trapped in cellulose-acetate films from which mRNA was obtained by grinding tissue & polyA selection; first-strand synthesis by anchored polyT primer set (Wang et al., 2000, Proc Natl Acad Sci U S A, 97, 4162-7) followed by polyG tailing, PCR amplification, restriction with NotI plus Apa1, size-selection and directional cloning in pBlueScriptIIKS. The NotI site represents the 3'-end of the mRNA. PHRED20 sequence clipped for vector sequences. Some clones might have chimeric inserts linked through Apa1 or NotI sites.

characteristic: description

text: appressorial, 5 hours after inoculation

characteristic: dev-stage

text: E. coli DH10B/r

text: Puccinia triticina

characteristic: organism

text: NotI

characteristic: strain

characteristic: title

text: Plasmid

text: pBSII(KS)

characteristic: vector


text: Agriculture and Agri-food Canada

text: live

text: Generic

text: urediniospores

text: mRNA obtained by grinding urediniospores & polyA selection; 'full-length' cloning attempted by 'CAP trapper method' (Maruyama and Sugano, 1994, Gene, 138, 171-4) and custom RNA-oligomer; first-strand synthesis by single polyT primer followed by PCR amplification, normalization, restriction with NotI plus Apa1, size-selection and directional cloning in pBlueScriptIIKS. The NotI site represents the 3'-end of the mRNA. PHRED20 sequence clipped for vector sequences. Some clones might have chimeric inserts linked through Apa1 or NotI sites.

text: resting urediniospores

text: E. coli DH10B/r

text: Puccinia triticina

text: NotI

text: Plasmid

text: pBSII(KS)


text: Agriculture and Agri-food Canada

text: live

text: Generic

text: urediniospores

text: urediniospores germinated for 16 hrs over water containing fresh wheat extract

text: E. coli DH10B/r

text: Puccinia triticina

text: NotI

text: Plasmid

text: pBSII(KS)


text: Agriculture and Agri-food Canada

text: live

text: Generic

text: urediniospores

text: urediniospores germinated for 16 hrs over water

text: E. coli DH10B/r

text: Puccinia triticina

text: NotI

text: Plasmid

text: pBSII(KS)


text: Agriculture and Agri-food Canada

text: live

text: Generic

text: mRNA obtained by grinding infected leaf tissue & polyA selection; 'full-length' cloning attempted by 'CAP trapper method' (Maruyama and Sugano, 1994, Gene, 138, 171-4) and custom RNA-oligomer; first-strand synthesis by single polyT primer followed by PCR amplification, normalization & subtraction with cDNAs from uninfected wheat at same developmental stage. Restriction of cDNAs with NotI plus Apa1, size-selection and directional cloning in pBlueScriptIIKS. The NotI site represents the 3'-end of the mRNA. PHRED20 sequence clipped for vector sequences. Some clones might have chimeric inserts linked through Apa1 or NotI sites.

text: haustorial, 6 days after inoculation

text: E. coli DH10B/r

text: Puccinia triticina

text: NotI

text: Plasmid

text: pBSII(KS)


text: Agriculture and Agri-food Canada

text: live

text: Generic

text: urediniospores

text: mRNA obtained by grinding urediniospores & polyA selection; 'full-length' cloning attempted by 'CAP trapper method' (Maruyama and Sugano, 1994, Gene, 138, 171-4) and custom RNA-oligomer; first-strand synthesis by anchored polyT primer set (Wang et al., 2000, Proc Natl Acad Sci U S A, 97, 4162-7) followed by PCR amplification. Restriction with NotI plus Apa1, size-selection and directional cloning in pBlueScriptIIKS. The NotI site represents the 3'-end of the mRNA. PHRED20 sequence clipped for vector sequences. Some clones might have chimeric inserts linked through Apa1 or NotI sites.

text: E. coli DH10B/r

text: Puccinia triticina

text: NotI

text: Plasmid

text: pBSII(KS)


text: Agriculture and Agri-food Canada

text: live

text: Generic

text: urediniospores

text: mRNA obtained by grinding urediniospores & polyA selection; first-strand synthesis by anchored polyT primer set (Wang et al., 2000, Proc Natl Acad Sci U S A, 97, 4162-7) followed by polyG tailing, PCR amplification, restriction with NotI plus Apa1, size-selection and directional cloning in pBlueScriptIIKS. The NotI site represents the 3'-end of the mRNA. PHRED20 sequence clipped for vector sequences. Some clones might have chimeric inserts linked through Apa1 or NotI sites.

text: urediniospores germinated for 2 or 16 hrs over water and then pooled

text: E. coli DH10B/r

text: Puccinia triticina

text: NotI

text: Plasmid

text: pBSII(KS)


text: Agriculture and Agri-food Canada

text: live

text: Generic

text: Re-sequenced insert from mixed up plates after microarray spotting. For most inserts: mRNA was obtained by grinding tissues & polyA selection; first-strand synthesis by anchored polyT primer set (Wang et al., 2000, Proc Natl Acad Sci U S A, 97, 4162-7) followed by polyG tailing, PCR amplification, restriction with NotI plus Apa1, size-selection and directional cloning in pBlueScriptIIKS. The NotI site represents the 3'-end of the mRNA. PHRED20 sequence clipped for vector sequences. Some clones might have chimeric inserts linked through Apa1 or NotI sites.

text: mixed stages (resting urediniospores; urediniospores germinated over water; appressorial stage on compatible wheat (Triticum aestivum); haustorial stage in

text: E. coli DH10B/r

text: Puccinia triticina

text: NotI

text: PTmix

text: Plasmid

text: pBSII(KS)


text: Medical School of Ribeirao Preto, University of Sao Paulo

text: live

text: Generic

text: A minilibrary was made by cloning products derived from ORESTES PCR (U.S.Letters Patent application No. 196,716 - Ludwig Institute for Cancer Research) profiles into the pUC 18 vector. Reverse transcription of whole animal mRNA and cDNA amplification were performed under low stringency conditions

text: Adult

text: Drosophila melanogaster

text: SmaI

text: SmaI

text: DA-ORESTES

text: pUC 18


text: live

text: Generic

text: Embryo

text: Drosophila melanogaster

text: SmaI

text: SmaI

text: DE-ORESTES

text: pUC 18


text: University of Alberta

text: live

text: Generic

text: Carmen

characteristic: cultivar

text: Plants were raised in growth chambers with a photoperiod of sixteen hours light and eight hours dark at 24oC and 50% humidity. At the start of the fourth-week following sowing, the outside of the stem above the second internode (counting up from the cotyledons) was scraped directly into a metal sieve over liquid N2. Total RNA was extracted using the Qiagen Maxi Total RNA kit, and polyA+ enriched RNA was obtained from this using Ambion Poly(A)Purist Mag. cDNA synthesis was primed using a NotI-oligodT primer, and using SalI adapters, double-stranded DNA was ligated into SalI NotI sites of pSPORT1.

text: 4 weeks post-sowing

text: stem

characteristic: organ

text: Cannabis sativa

text: SalI

text: NotI

text: cortical regions including cambium, phloem, bast fibers

characteristic: tissue-type

text: stem phloem (bast) fiber enriched library CS01

text: pSPORT 1


text: United States Department of Agriculture

text: live

text: Generic

text: Gale Gala

text: Malus domestica

text: young leaf

text: Erwinia amylovora challenged cDNA suppression subtractive hybridization library late upregulated genes


text: The Samuel Roberts Noble Foundation

text: live

text: Generic

text: Guar (Cyamopsis tetragonoloba) plants, cultivar HES 1401, were grown in 2 gallon pots containing 75% Metro Mix 350 soil and 25% sand at a temperature of 26oC/22oC (day/night). Plants were fertilized at time of watering using a commercial Peters Professional 20-10-20 (N-P-K) fertilizer mix. Seeds were harvested 15, 20, 25, 30, 35, and 40 days after flowering (DAF). cDNA was prepared from polyA+ enriched, pooled samples of equivalent amounts of total RNA from each time point. An 'early' seed library was generated from seeds 15, 20, and 25 DAF. The cDNA was directionally ligated into the Uni-Zap XR vector and packaged using Gigapack III Gold packaging extracts. Phagemids containing cDNA inserts were in vivo excised from the recombinant Uni-ZAP XR vector using ExAssist helper phage and the E. coli strain XL1-Blue MRF'. Excised plasmids were plated using SOLR cells.

text: early developmental stage (15-25 days after flowering, DAF)

text: Cyamopsis tetragonoloba

text: EcoRI (GAATTC)

text: XhoI (CTCGAG)

text: seed

text: Cyamopsis tetragonoloba (L.) Taub seeds at early developmental stage

text: pBluescript sk


text: The Samuel Roberts Noble Foundation

text: live

text: Generic

text: Guar (Cyamopsis tetragonoloba) plants, cultivar HES 1401, were grown in 2 gallon pots containing 75% Metro Mix 350 soil and 25% sand at a temperature of 26oC/22oC (day/night). Plants were fertilized at time of watering using a commercial Peters Professional 20-10-20 (N-P-K) fertilizer mix. Seeds were harvested 15, 20, 25, 30, 35, and 40 days after flowering (DAF). cDNA was prepared from polyA+ enriched, pooled samples of equivalent amounts of total RNA from each time point. A 'late' seed library was generated from seeds 30, 35, and 40 DAF. The cDNA was directionally ligated into the Uni-Zap XR vector and packaged using Gigapack III Gold packaging extracts. Phagemids containing cDNA inserts were in vivo excised from the recombinant Uni-ZAP XR vector using ExAssist helper phage and the E. coli strain XL1-Blue MRF'. Excised plasmids were plated using SOLR cells.

text: late developmental stage (30-40 days after flowering, DAF)

text: Cyamopsis tetragonoloba

text: EcoRI (GAATTC)

text: XhoI (CTCGAG)

text: seed

text: Cyamopsis tetragonoloba (L.) Taub seeds at late developmental stage

text: pBluescript sk


text: AgResearch

text: live

text: Generic

text: Neotyphodium lolii strain Lp19 was grown for 10 days in potato dextrose broth with shaking. Mycelia was harvested and used to prepare RNA and mRNA for library construction using lambda TriplEX2 vector (Clontech).

text: Epichloe festucae var. lolii

text: Lambda TriplEx2


text: AgResearch

text: live

text: Generic

text: Neotyphodium lolii strain Lp19 was grown for 14 days in potato dextrose broth, then 19 days in Blankenship Minimal medium with shaking. Mycelia was harvested and used to prepare RNA and mRNA for library construction using lambda TriplEX2 vector (Clontech).

text: Epichloe festucae var. lolii

text: Lambda TriplEx2


text: RIKEN Center for Developmental Biology

text: live

text: Generic

text: adult

text: Idiosepius paradoxus

text: brain

text: Northern pygmy squid cDNA library


text: McGill University

text: live

text: Generic

text: SSH cDNA library was prepared from RNA of solanum tuberosum and Rhizoctonia solani AG3 in interaction as tester, and RNA of Rhizoctonia solani AG3 growing alone and not infected potato sprout as driver. The cDNA isolated with SSH were cloned in the vector TOPO-PcR2.1. The inserts were amplified by PCR and the corresponding amplicons were printed on microarray slides. The up-regulated genes were identified by microarray. The organism where the gene come from have been verified by PCR.

characteristic: isolate

text: Rhizoctonia solani AG-3

text: AG 3

text: Rhizoctonia solani AG3 SSH up-regulated cDNA library during interaction with Solanum tuberosum

text: plasmid

text: TOPO pcr2.1


characteristic: External Id

text: National Botanical Research Institute Lucknow-226001 India

text: live

text: Generic

text: Trichosanthes dioica

text: Trichosanthes dioica suppression subtractive hybridization


text: Dalhousie University

characteristic: INSDC center name

text: live

text: Generic

text: Euglena gracilis

text: Non-normalized Euglena gracilis cDNA


text: Dalhousie University

characteristic: INSDC first public

text: live

text: Generic

text: Euglena gracilis

text: Normalized long fraction Euglena gracilis cDNA


text: University of California, Santa Cruz

characteristic: INSDC last update

text: live

text: Generic

text: spliced sequence from pcr clones.

text: Saccharomyces cerevisiae

text: S. cerevisiae RT-PCR, in pCR-TOPOII


text: Pusan University

text: live

characteristic: INSDC status

text: Generic

text: 3rd stage larva

text: Anisakis simplex

text: Anisakis simplex 3rd stage larva cDNA library


text: Yeungnam University

text: live

text: Generic

characteristic: NCBI submission model

text: Adipocyte After differentiation

characteristic: cell type

text: piglet (about two weeks old)

text: Sus scrofa

text: Male

characteristic: sex

text: Landrace

text: Neckfat

text: Sus Scrofa Adipocyte Zap Express Library After differentiation

text: pBK-CMV


text: Yeungnam University

text: live

text: Generic

characteristic: NCBI submission package

text: Adipocyte Before differentiation

text: piglet (about two weeks old)

characteristic: dev-stage

text: Sus scrofa

text: Male

text: Landrace

text: Neckfat

text: Sus Scrofa Adipocyte Zap Express Library Before differentiation

text: pBK-CMV


text: United States Department of Agriculture

text: live

text: Generic

text: Gale Gala

text: Malus domestica

characteristic: organism

text: young leaf

text: Erwinia amylovora challenged cDNA suppression subtractive hybridization library upregulated genes at 1 hour


text: Warwick University

text: live

text: Generic

text: Brassica oleracea var. alboglabra

text: etiolated seedling

characteristic: title


text: Agriculture and Agri-food Canada

text: live

text: Generic

text: Chinese Spring addition line contain. chom 7E from T. elongata

text: Wheat plants (Chinese Spring and Chinese Spring addition line containing chromosome 7E from Thinopyrum elongatum) were grown under a 16 h light (20oC) /8 h dark (16oC) regime until mid-anthesis. Heads at mid-anthesis of the addition line were point-inoculated with Fg spores (10 ul of 1 X 105 spores/ml) at every fully developed spikelet. Water inoculation was used on Chinese Spring plants. Inoculated spikelets (with Fg or water) were harvested immediately after inoculation. Total RNA was extracted using guanidinium thiocyanate followed by pelleting through CsCl2. Poly(A+)RNA was isolated following the manufacturer's instructions using MPG Streptavidin Biotinylated Oligo (dT)25 Complex (CPG, NJ). A subtraction suppression hybridization (SSH) library was generated with the PCR-Select cDNA Subtraction Kit (BD Biosciences, Missisauga, ON, Canada), using cDNA from F. graminearum-inoculated tissues of the addition line as the tester, subtracted with water-inoculated cDNA from Chinese Spring as the driver. The enriched SSH PCR products were cloned into the pGEM T-easy vector (Promega).

characteristic: description

text: anthesis

text: E. coli Sure Cells

characteristic: lab-host

text: Triticum aestivum

text: EcoRI

text: EcoRI

characteristic: strain

text: spikelets

text: Ta09_AAFC_ECORC_Fusarium_graminearum_inculated_wheat_heads

text: Plasmid

text: pGem-T easy

characteristic: vector


text: Agriculture and Agri-food Canada

text: live

text: Generic

characteristic: cultivar

text: Wheat plants (Chinese Spring and Chinese Spring addition line containing chromosome 7E from Thinopyrum elongatum) were grown under a 16 h light (20oC) /8 h dark (16oC) regime until mid-anthesis. Heads at mid-anthesis of the addition line were point-inoculated with Fg spores (10 ul of 1 X 105 spores/ml) at every fully developed spikelet. Water inoculation was used on Chinese Spring plants. Inoculated plants were misted for 10- second intervals every 15 min for 2 days. Inoculated spikelets (with Fg or water) were harvested 4 days after inoculation. Total RNA was extracted using guanidinium thiocyanate followed by pelleting through CsCl2. Poly(A+)RNA was isolated following the manufacturer's instructions using MPG Streptavidin Biotinylated Oligo (dT)25 Complex (CPG, NJ). A subtraction suppression hybridization (SSH) library was generated with the PCR-Select cDNA Subtraction Kit (BD Biosciences, Missisauga, ON, Canada), using cDNA from F. graminearum-inoculated tissues of the addition line as the tester, subtracted with water-inoculated cDNA from Chinese Spring as the driver. The enriched SSH PCR products were cloned into the pGEM T-easy vector (Promega).

text: 4 days after anthesis

text: E. coli Sure Cells

text: Triticum aestivum

text: EcoRI

text: EcoRI

text: spikelets

characteristic: tissue-type

text: Ta10_AAFC_ECORC_Fusarium_graminearum_inculated_wheat_heads

text: Plasmid

text: pGem-T easy


characteristic: External Id

text: Agriculture and Agri-food Canada

characteristic: INSDC center name

text: live

text: Generic

text: 4 days after anthesis

text: E. coli Sure Cells

text: Triticum aestivum

text: EcoRI

text: EcoRI

text: spikelets

text: Ta10b_AAFC_ECORC_Fusarium_graminearum_inculated_wheat_heads

text: Plasmid

text: pGem-T easy


text: Agriculture and Agri-food Canada

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: 4 days after anthesis

text: E. coli Sure Cells

text: Triticum aestivum

text: EcoRI

text: EcoRI

text: spikelets

text: Ta10c_AAFC_ECORC_Fusarium_graminearum_inculated_wheat_heads

text: Plasmid

text: pGem-T easy


text: Centre for Cellular and Molecular Biology

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: selectn-12 devel. Central Coffee Res. Inst., Chikamagalur,India

text: The library was made using mRNA isolated from moisture-stressed leaf tissue using Clonetch SMART-TM PCR cDNA Synthesis Kit.The cloned fragments were amplified from the recombinant plasmid DNA using M13 universal primers and were sequenced on an automated DNA sequencer ABI3700 using fluorescence based dideoxy terminator chemistry.

text: Coffea arabica

text: moisture-stressed leaf tissue obtained from Department of Plant Physiology, University of Agricultural Sciences, Bangalore, India.

text: Coffea arabica EST library

text: pMOSBlue Blunt End vector


text: The University of Iowa

text: live

text: Generic

text: CMRC-CP-II1 is a normalized library derived from CMRC-CP-II0. The library was normalized and constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CCTGAATAG. Tissue was obtained from the Provasoli-Guillard National Center for Culture of Marine Phytoplankton (CCMP).

text: DH10B T1 phage resistant (Invitrogen)

text: Cyanophora paradoxa

text: EcoR I

text: Not I

text: CCMP 329

text: pT7T3-Pac (Pharmacia) with a modified polylinker


text: USDA - ARS

text: live

text: Generic

text: A high quality EST with at least 100 contiguous bases at Trace Tuner score of 20 or better.

text: Adult

text: E. coli XL1-Blue

text: Lysiphlebus testaceipes

text: EcoRI

text: XhoI

text: male and female

characteristic: sex

text: Whole body

text: Lysiphlebus testaceipes adult whole body

text: pBluescript II SK+


text: University of St Andrews

text: live

text: Generic

characteristic: NCBI submission package

text: Salmo salar

text: Fast muscle

text: Forward subtracted library from fast muscle of Salmo salar


text: United States Department of Agriculture

text: live

text: Generic

text: Gale Gala

text: Malus domestica

text: young leaf

text: Erwinia amylovora challenged cDNA suppression subtractive hybridization library down-regulated genes at 2 hours


text: United States Department of Agriculture

text: live

text: Generic

text: Gale Gala

text: Malus domestica

text: young leaf

text: Erwinia amylovora challenged cDNA suppression subtractive hybridization library down-regulated genes at 12 hours


text: United States Department of Agriculture

text: live

text: Generic

text: Gale Gala

text: Malus domestica

text: young leaf

text: Erwinia amylovora challenged cDNA suppression subtractive hybridization library down-regulated genes at 24 hours


text: United States Department of Agriculture

text: live

text: Generic

text: Gale Gala

text: Malus domestica

text: young leaf

text: Erwinia amylovora challenged cDNA suppression subtractive hybridization library down-regulated genes at 48 hours


text: United States Department of Agriculture

text: live

text: Generic

text: Gale Gala

text: Malus domestica

text: young leaf

text: Erwinia amylovora challenged cDNA suppression subtractive hybridization library down-regulated genes at 1 hour


text: Centro de Pesquisas Rene Rachou - FioCruz

text: live

text: Generic

text: Trypanosoma cruzi

text: Tehuantepec 17LER

text: SM-Lib-Tc Tehuantepec 17LER


text: live

text: Generic

text: Trypanosoma cruzi

text: Y BZR

text: SM-Lib-Tc Y BZR


text: live

text: Generic

text: Trypanosoma cruzi

text: Tehuantepec 17WTS

text: SM-Lib-Tc Tehuantepec 17WTS


text: James Cook University

text: live

text: Generic

text: Isolated from the coral Acropora aspera. Symbiodinium were removed from the coral skeleton and then harvested by centrifugation, washed four time in filtered sea water before being ground under liquid nitrogen and resuspended in RLT buffer (Qiagen RNA Midi Prep Kit). Cells were then spun through a Qia-Shredder (Qiagen) and snap frozen in liquid nitrogen for storage. Total RNA was purified for each sample using the Qiagen mRNA Midi Prep Kit, equal amounts of total RNA from each treatment (control, short term heat stress, long term heat stress, ammonium enriched and isolated/Ci treated) were mixed and the mRNA was purified using a Poly A Purist kit (Ambion). The purified mRNA was then used to construct a directional phage library in lambda-ZAP II. Phagemids were generated and the library was normalised using the method of Bonaldo et al. (1996) and clones were sequenced directionally using the M13 Reverse primer.

text: Cladocopium sp. C3

text: EcoRI

text: XhoI

text: Symbiodinium normalized stress library Lambda ZAP II

text: Phagemid

text: pBluescript SK-


text: Cardiff University

text: live

text: Generic

text: RNA was prepared from pre-metamorphic and post-metamorphic halibut larvae and a subtractive library was constructed which was enriched in transcripts at the climax of metamorphosis.

text: Metamorphosis

characteristic: dev-stage

text: Hippoglossus hippoglossus

text: Male and female

text: Halibut larval subtractive library

text: Plasmid

text: pGEM-T


text: Academia Sinica

text: live

text: Generic

text: Shishi

text: cDNA prepared from rice young panicle mRNA by using oligo(dT) as the primer for ligation into the plasmid of pGEM-T Easy.

text: 40 days after flowering

text: Glycine max

characteristic: organism

text: endosperm tissue in developing seeds

text: Soybean endosperm tissue in developing seeds

text: pGEM-T Easy


text: University of Aberdeen

text: live

text: Generic

text: country: United Kingdom:Scotland

text: Salmo salar

text: Salmo salar after live bacterial vaccine

characteristic: title


text: University of Aberdeen

text: live

text: Generic

text: Identified following 2 weeks food withdrawal by subtractive cloning. country: United Kingdom:Scotland

characteristic: description

text: Salmo salar

text: Salmo salar after short term starvation


text: Universidade Federal de Goias

text: live

text: Generic

text: Paracoccidioides brasiliensis

text: Paracoccidioides brasiliensis transition library

text: Plasmid


text: Institute of Ramie, Hunan Agriculture University

text: live

text: Generic

text: Boehmeria nivea

text: bark

text: Bark cDNA library of ramie Boehmeria nivea (L.) Gaud


text: Genoscope - Centre National de Sequencage

text: live

text: Generic

text: juvenile versus mature wood and mature versus juvenile wood SSH libraries. EST libraries available at Transcriptional Regulation and Wood Formation team, UMR UPS-CNRS 5546, 31 326 Castanet Tolosan, France (E-mail : sivadon@scsv.ups-tlse.fr - Web : www. ups-tlse.fr). country: Portugal

text: Eucalyptus globulus

text: RAIZ clone VC9

text: differentiating xylem

text: Eucalyptus globulus differentiating xylem RAIZ clone VC9


text: live

text: Generic

text: Eucalyptus globulus subsp. bicostata Maiden. microgravity versus gravity subtracted library. EST library available at Transcriptional Regulation and Wood Formation team, UMR UPS-CNRS 5546, 31 326 Castanet Tolosan, France (E-mail : sivadon@scsv.ups-tlse.fr - Web : www.ups-tlse.fr)

text: seedlings grown under 14, 28 and 45 days of clinostat conditions

text: Eucalyptus globulus subsp. bicostata

text: leaves, stems, roots

text: Eucalyptus globulus microgravity versus gravity subtracted library


text: live

text: Generic

text: Eucalyptus globulus subsp. bicostata Maiden. gravity versus microgravity subtracted library. EST library available at Transcriptional Regulation and Wood Formation team, UMR UPS-CNRS 5546, 31 326 Castanet Tolosan, France (E-mail : sivadon@scsv.ups-tlse.fr - Web : www.ups-tlse.fr)

text: Eucalyptus globulus subsp. bicostata

text: leaves, stems, roots

text: Eucalyptus globulus gravity versus microgravity subtracted library


text: live

text: Generic

text: cDNA library available at Transcriptional Regulation and Wood Formation team, UMR UPS-CNRS 5546, 31 326 Castanet Tolosan, France (E-mail : sivadon@scsv.ups-tlse.fr - Web : www.ups-tlse.fr). country: France:Midi-Pyrenees, Longages

text: Eucalyptus gunnii

text: EcoRI

text: XhoI

text: AFOCEL clone 832

text: differentiating xylem

text: Eucalyptus gunnii differentiating xylem AFOCEL clone 832

text: Lambda ZapII, Bluescript SK-


text: Embrapa Recursos Geneticos e Biotecnologia

text: live

text: Generic

text: Arachis stenosperma seeds were germinated in sterile soil. Roots inoculated with Bradirhyzobium sp. were collected from three-month old plants. Collected materials were immediately frozen in liquid nitrogen for RNA extraction. Total RNA was isolated from plant materials using Trizol Reagent (Invitrogen, Carlsbad, CA, USA), according to the manufacture's instructions. Poly (A)+ RNA was extracted from 1 mg of total RNA using the Oligotex Spin Column (Qiagen Inc., Valencia, CA, USA) according to the manufacture's protocol. Full-length cDNA libraries were constructed using the SMART cDNA synthesis kit in lambdaTriplEx2 (Clontech, Palo Alto, CA, USA). The resulting cDNA was packed into lambda phages using the Gigapack III Gold packaging kit (Stratagene, La Jolla, CA, USA). The pTriplEx2 phagemid clones in Escherichia coli were obtained using the mass in-vivo excision protocol according to the manufacture`s instructions (Clontech, USA). The white clones grown on screening LB medium (Amp/IPTG/X-Gal) were recovered by random colony selection. All cDNA inserts were sequenced from the 5'-end using specifically designed primer: PT2F2 5'-GCGCCATTGTGTTGGTACCC-3'.

text: Mature plant

text: Accession V10309 (in Embrapa Genetic Resources and Biotechnology Germplasm bank)

characteristic: isolate

text: Root

characteristic: organ

text: Arachis stenosperma

text: Root

text: Roots inoculated with Bradirhyzobium sp. (RI)

text: Phage

text: pTriplEx2 (Clontech, Palo Alto, CA, USA).


text: live

text: Generic

text: Arachis stenosperma seeds were germinated in sterile soil. Healthy roots were collected from three-month old plants. Collected materials were immediately frozen in liquid nitrogen for RNA extraction. Total RNA was isolated from plant materials using Trizol Reagent (Invitrogen, Carlsbad, CA, USA), according to the manufacture's instructions. Poly (A)+ RNA was extracted from 1 mg of total RNA using the Oligotex Spin Column (Qiagen Inc., Valencia, CA, USA) according to the manufacture's protocol. Full-length cDNA libraries were constructed using the SMART cDNA synthesis kit in lambdaTriplEx2 (Clontech, Palo Alto, CA, USA). The resulting cDNA was packed into lambda phages using the Gigapack III Gold packaging kit (Stratagene, La Jolla, CA, USA). The pTriplEx2 phagemid clones in Escherichia coli were obtained using the mass in-vivo excision protocol according to the manufacture`s instructions (Clontech, USA). The white clones grown on screening LB medium (Amp/IPTG/X-Gal) were recovered by random colony selection. All cDNA inserts were sequenced from the 5'-end using specifically designed primer: PT2F2 5'-GCGCCATTGTGTTGGTACCC-3'.

text: Mature plant

text: Root

text: Arachis stenosperma

text: Root

text: Healthy Roots (RN)

text: Phage

characteristic: vector


text: live

text: Generic

text: Arachis stenosperma seeds were germinated in sterile soil. Roots inoculated with 10.000 juveniles (J2) Meloidogyne arenaria were collected from three-month old plants. Collected materials were immediately frozen in liquid nitrogen for RNA extraction. Total RNA was isolated from plant materials using Trizol Reagent (Invitrogen, Carlsbad, CA, USA), according to the manufacture's instructions. Poly (A)+ RNA was extracted from 1 mg of total RNA using the Oligotex Spin Column (Qiagen Inc., Valencia, CA, USA) according to the manufacture's protocol. Full-length cDNA libraries were constructed using the SMART cDNA synthesis kit in lambdaTriplEx2 (Clontech, Palo Alto, CA, USA). The resulting cDNA was packed into lambda phages using the Gigapack III Gold packaging kit (Stratagene, La Jolla, CA, USA). The pTriplEx2 phagemid clones in Escherichia coli were obtained using the mass in-vivo excision protocol according to the manufacture`s instructions (Clontech, USA). The white clones grown on screening LB medium (Amp/IPTG/X-Gal) were recovered by random colony selection. All cDNA inserts were sequenced from the 5'-end using specifically designed primer: PT2F2 5'-GCGCCATTGTGTTGGTACCC-3'.

text: Mature plant

text: Root

text: Arachis stenosperma

text: Root

text: Roots inoculated with Meloidogyne arenaria (RM)

text: Phage


text: live

text: Generic

text: Arachis stenosperma seeds were germinated in sterile soil. Healthy leaves were collected from three-month old plants. Collected materials were immediately frozen in liquid nitrogen for RNA extraction. Total RNA was isolated from plant materials using Trizol Reagent (Invitrogen, Carlsbad, CA, USA), according to the manufacture's instructions. Poly (A)+ RNA was extracted from 1 mg of total RNA using the Oligotex Spin Column (Qiagen Inc., Valencia, CA, USA) according to the manufacture's protocol. Full-length cDNA libraries were constructed using the SMART cDNA synthesis kit in lambdaTriplEx2 (Clontech, Palo Alto, CA, USA). The resulting cDNA was packed into lambda phages using the Gigapack III Gold packaging kit (Stratagene, La Jolla, CA, USA). The pTriplEx2 phagemid clones in Escherichia coli were obtained using the mass in-vivo excision protocol according to the manufacture`s instructions (Clontech, USA). The white clones grown on screening LB medium (Amp/IPTG/X-Gal) were recovered by random colony selection. All cDNA inserts were sequenced from the 5'-end using specifically designed primer: PT2F2 5'-GCGCCATTGTGTTGGTACCC-3'.

text: Mature plant

text: Leaf

text: Arachis stenosperma

text: Leaf

text: Healthy Leaves (ML)

text: Phage


text: live

text: Generic

text: Total RNA samples were prepared individually from each tissue, checked for quality, then pooled for construction and normalization of cDNA library by Invitrogen. Plasmids were isolated and inserts end-sequenced by the Marine DNA Sequencing and Analysis Facility at Mount Desert Island Biological Laboratory. Traces were processed for submission to dbEST by trace2dbest software (Parkinson, Anthony and Blaxter, unpublished software).

text: Adult intermolt and postmolt

text: Homarus americanus

text: Gill, epipodite, branchiostegite, heart, ovary, testis, antennal gland, abdominal muscle, hepatopancreas, brain

text: Lobster Multiple Tissues, Normalized

text: pCMV Sport 6.1


text: NORTHEAST FORESTRY UNIVERSITY

text: live

text: Generic

text: Tamarix hispida were treated with NaHCO3 for 0, 24 and 52 hours respectively before cDNA library construction.

text: Tamarix hispida

text: sfiI

text: sfiI

text: leaf

text: cDNA library of Tamarix hispida leaves at different stress times

text: Plasmid

text: pDNR-LIB


text: Centro de Energia Nuclear na Agricultura, Universidade de Sao Paulo

text: live

text: Generic

text: 'CAB 214'

characteristic: cultivar

text: SSH cDNA libraries enriched for sequences differentially expressed during the T. cacao and C. perniciosa interaction from resistant genotype. SSH library was constructed using as tester a pooled cDNA from all sampling periods after inoculation of the resistant genotype (CAB 214), subtracted with a similar driver derived from the inoculated susceptible genotype (ICS 39)

text: 24 h; 48 h; 72 h; 120 h; and 240 h after inoculation

text: Theobroma cacao

text: infected shoot-tips

text: pGEM-T easy vector


text: live

text: Generic

text: 'ICS 39'

text: SSH cDNA libraries enriched for sequences differentially expressed during the T. cacao and C. perniciosa interaction from susceptible genotype. SSH library was constructed using as tester a pooled cDNA from all sampling periods after inoculation of the susceptible genotype (ICS 39), subtracted with a similar driver derived from the inoculated resistant genotype (CAB 214)

text: Theobroma cacao

text: infected shoot-tips

characteristic: tissue-type

text: pGEM-T easy vector


characteristic: External Id

text: IFAPA-CICE, Junta de Andalucia

text: live

text: Generic

text: Total RNA was isoltated from roots harvested 15, 21 and 35 days after inoculation with O. crenata. cDNA was obtained from total RNA using the SMART PCR cDNA system (Clontech). This cDNA was used as tester probe in Supression Substractive Hybridization (SSH) according to the manufacture's instructions. The SSH-cDNA fragments were ligated into the pGEM-T easy vector (Promega). Plasmids containing cDNA inserts were cloned in E. coli XL1-Blue MRF' cells (Stratagene)

text: 15, 21 and 35 days after inoculation with Orobanche crenata

text: Medicago truncatula

text: root

text: MTOROCRE

text: Plasmid

text: pGEM-T easy


text: Stanford University

characteristic: INSDC center name

text: live

text: Generic

text: Library made from nitrogen starved cells

text: Aureococcus anophagefferens

text: CCMP 1984

characteristic: strain

text: CloneMiner (cat#11144-010)


text: NRC on Plant Biotechnology, IARI

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Pusa 362

text: Drought stress up-regulated SSH-cDNA library prepared from flowering stage chickpea plant tissue in pGEMT easy vector.

text: Flowering stage (about 3 months old)

text: Cicer arietinum

text: subtracted cDNA library of drought stressed chickpea

text: pGEM-T EASY


text: NIBGE-Pakistan

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: country: Pakistan

text: Gossypium hirsutum

text: cotton fiber

text: Gossypium hirsutum cotton fiber


text: The Institute for Genomic Research (TIGR; www.tigr.org)

text: live

text: Generic

characteristic: NCBI submission package

text: Perkinsus marinus

text: Perkinsus marinus TXsc (S-) cDNA library

text: Plasmid

text: pBluescript KS


text: live

text: Generic

text: Perkinsus marinus

text: Perkinsus marinus TXsc (S+) cDNA library

text: Plasmid

text: pBluescript KS


text: live

text: Generic

text: Clone Pm CV4CB5 D4

characteristic: cell-line

text: Perkinsus marinus

text: EcoRV

text: NotI

text: Perkinsus marinus small insert cDNA library

text: Plasmid


text: live

text: Generic

text: Clone Pm CV4CB5 D4

text: Perkinsus marinus

characteristic: organism

text: EcoRV

text: NotI

text: Perkinsus marinus large insert cDNA library

text: Plasmid


text: United States Department of Agriculture

text: live

text: Generic

text: Gale Gala

text: Malus domestica

text: young leaf

text: Erwinia amylovora challenged cDNA suppression subtractive hybridization library up-regulated genes at 2 hours

characteristic: title


text: United States Department of Agriculture

text: live

text: Generic

text: Gale Gala

text: Malus domestica

text: young leaf

text: Erwinia amylovora challenged cDNA suppression subtractive hybridization library up-regulated genes at 12 hours


text: Brooms Barn Research

text: live

text: Generic

text: Regina

text: HvH Unigenes from 3 day old Hordeum vulgaris (cv. Regina) roots challenged with Polymyxa graminis zoospores (host interaction) 15 minute to seven hour post inoculation. The term 'Unigenes' refers to the fact that these ESTs are representative of the unique gene sequences found in each library.

characteristic: description

text: 3 day old

characteristic: dev-stage

text: Hordeum vulgare

text: HvH


text: Brooms Barn Research

text: live

text: Generic

text: Regina

text: HvNH Unigenes from 3 day old Hordeum vulgaris (cv. Regina) roots challenged with Polymyxa betae zoospores (non-host interaction) 15 minute to seven hour post inoculation. The term 'Unigenes' refers to the fact that these ESTs are representative of the unique gene sequences found in each library.

text: 3 day old

text: Hordeum vulgare

text: HvNH


text: Graduate School of Science, University of Tokyo; CREST, Japan Science and Technology Corporation, Japan

text: live

text: Generic

text: late gastrula (stage 12.5)

text: Xenopus laevis

text: anterior endomesoderm

text: Osada Taira anterior endomesoderm (AEM) pCS105 cDNA library


text: RIKEN Center for Developmental Biology

text: live

text: Generic

text: adult

text: Elaphe quadrivirgata

text: brain

text: Japanese four-striped rat snake cDNA library


text: Institute of Microbiology, Chinese Academy of Sciences

text: live

text: Generic

text: The library was constructed using PCR-select cDNA subtraction method with 9 dpa cotton fiber as tester and leaf as driver

text: 9 days post anthesis (dpa)

text: Gossypium hirsutum

text: fiber

text: Cotton fiber suppression subtractive hybridization cDNA library


text: Rosetta Inpharmatics

text: live

text: Generic

text: RT-PCR products were amplified using gene-specific primers and cloned directly following instructions from the vendor

text: Homo sapiens

text: adipose

text: ACC2 library

text: Plasmid - uses TOPO TA Cloning

text: pCR2.1-TOPO vector (Invitrogen)


text: The University of Tokyo

text: live

text: Generic

text: Saccharomyces cerevisiae

text: vector-capped budding yeast cDNA library, strain SK1


text: The University of Tokyo

text: live

text: Generic

text: Saccharomyces cerevisiae

text: vector-capped budding yeast cDNA library, strain S288C


text: National Institute for Basic Biology

text: live

text: Generic

text: mixture of equivalent stages 10.5-15 (early gastrula to mid neurula)

text: Xenopus laevis

text: Keller explants

text: Yamamoto/Hyodo-Miura NIBB/NBRP Xenopus DMZ pCS2p+ cDNA library


text: NIDCD/NIH

text: live

text: Generic

text: Poly A+ RNAs were extracted from circumvallate taste papillae (tester) and non-taste lingual epithelium (driver). Following a pre-amplification step using the Smart PCR cDNA Synthesis kit (K1052-1, BD Biosciences), circumvallate papillae cDNA was subtracted against non-taste lingual epithelium cDNA using the PCR-Select cDNA subtraction kit (K1804-1, BD Biosciences) according to manufacturer's protocols, which are available in detail at http://www.clontech.com/. Subtracted cDNAs were non-directionally cloned into the T/A cloning site of pCRII (Invitrogen). The sequencing of individual clones, and EST analysis were performed at the NIH Intramural Sequencing Center (NISC). Analyzed data available through http://neibank.nei.nih.gov.

text: adult

characteristic: lab-host

text: tongue

characteristic: organ

text: Mus musculus

text: taste buds

text: mouse taste receptor cell, subtracted, normalized

text: Plasmid

text: pCRII


text: School of Life Sciences, Hubei University

text: live

text: Generic

text: country: China. biotype II. Suppression subtraction library created for larvae incubated for 24 hours on rice

text: forth instar larva

text: Oryza sativa cv. TN1

text: Nilaparvata lugens

text: Nilaparvata lugens forth instar larva, host Oryza sativa cv. TN1


text: live

text: Generic

text: forth instar larva

text: Oryza sativa cv. B5

text: Nilaparvata lugens

text: Nilaparvata lugens forth instar larva, host Oryza sativa cv. B5


text: Genome Institute of Singapore

text: live

text: Generic

text: Priming method: GsuI-d(T) priming ; Priming sequence: 5' GAGCTAGTTCTGGAG(T)16VN ; Directionally cloned, 5' cloning site: NotI site GCGGCCGC ; 5' linker/adaptor sequence: 5' GGATCCGAC 3' ; 3' cloning site: BseRI ; Insert size range: 2.5kbp-700bp ; For PCR insert analysis: M13 forward and reverse primers ; Library is not amplified ; Recombinants (inserts): 94% ; Library complexity: 6.38 X 106 cfu ; Full-length construction (method): Ng et al, Nature Method 2005 2(2):105-111 The pooled tissue RNA was collected and used to construct full length enriched cDNA library and also served as template to synthesize complex first strand cDNA probe. Two high density colony arrays were made from over 110K cDNA clones and hybridized with the probes. Low abundant clones were selected by the Colony Array Subtractive Hybridization (CASH) analysis as they represented less abundantly expressed clones. The hybridization intensities for all clones span from 0 to 1 million counts and the low abundant selected clones in this library ranges from 0 to 45,000.

text: Adult fish of 3-4 months old

text: Escherichia Top10

text: Brain

text: Danio rerio

text: BseRI

text: Not I

text: Males and females mixed

characteristic: sex

text: Tubingen (TU)

text: Brain (including tissues and cartilage around brain)

text: Plasmid

characteristic: vector


characteristic: External Id

text: University of Birmingham

text: live

text: Generic

text: Ricinus communis

text: hypocotyl phloem

characteristic: tissue-type

text: Ricinus phloem library


text: University of Padova

characteristic: INSDC center name

text: live

text: Generic

text: country: Italy:Venice Lagoon

text: juvenile

text: Zosterisessor ophiocephalus

text: BstXI

text: NotI

text: wild

characteristic: strain

text: gills, liver, brain, eye, spleen, muscle, intestine walls

text: ZOPD

text: plasmid

text: pcDNAII (Invitrogen)


text: Huazhong Agricultural University

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Citrus sinensis

text: Different expression profile of FJWC and FJ72-1


text: Kyoto University, Dept. of Plant Gene & Totipotency

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: cultured cells selected for the high production of berberine

text: Coptis japonica var. dissecta

text: cDNA library of high berberine-producing cells in pDR196


text: University of Bath

text: live

text: Generic

characteristic: NCBI submission package

text: Hemocyte

characteristic: cell type

text: 5th Instar Larvae

text: Manduca sexta

text: BsrGI

text: BsrGI

text: Hemocyte

text: MS EST Hem

text: Plasmid


text: Duke University Medical Center - Department of Neurobiology

text: live

text: Generic

text: The library was constructed as described by Carninci, P. (2003) in DNA Microarrays: A Molecular Cloning Manual, eds. Bowtell, D. & Sambrook, J. (Cold Spring Harbor, NY), pp. 647-670.

text: juvenile

text: brain

text: Taeniopygia guttata

characteristic: organism

text: EcoRI

text: BamHI

text: brain

text: library 65 - subtracted (Single juvenile learning minus 2 adult male singers)

characteristic: title

text: Plasmid


text: Reading University

text: live

text: Generic

text: Lumbricus rubellus from control site, exposed to Pb spiked soil.

characteristic: description

text: Adult

characteristic: dev-stage

text: Lumbricus rubellus

text: Hermaphrodite

text: pBluescript II SK(+)


text: Reading University

text: live

text: Generic

text: Lumbricus rubellus collectd from field site Cwmystwyth Cottage.

text: Adult

text: Lumbricus rubellus

text: Hermaphrodite

text: pBluescript II SK(+)


text: Northeast Forestry University

text: live

text: Generic

text: Puccinellia tenuiflora

text: leaf

text: SSH cDNA Library of Puccinellia tenuiflora


text: Universidad Nacional del Sur

text: live

text: Generic

text: SMART PCR cDNA synthesis kit (Clontech, USA). pGEM-T Easy vector system I (Promega, USA). E.coli XL10-Gold ultracompetent cells (Stratagene, USA).

text: 2n = 2x = 20

text: Eragrostis curvula

text: panicles


text: Universidad Nacional del Sur

text: live

text: Generic

text: 2n = 4x = 40

text: Eragrostis curvula

text: panicles


text: Universidad Nacional del Sur

text: live

text: Generic

text: 2n = 4x = 40

text: Eragrostis curvula

text: leaf


text: BC Cancer Agency

text: live

text: Generic

text: 8.5 months

text: ElectroMAX DH10B T1 Phage-Resistant Cells

characteristic: lab-host

text: Ovary

characteristic: organ

text: Bos taurus

text: SalI (5' end of cDNA)

text: NotI (3' end of cDNA)

text: female

text: Hereford

text: Ovary

text: pCMV SPORT 6.0


text: Institute Grassland & Environmental Res

text: live

text: Generic

text: Trifolium repens

text: leaf

text: Trifolium repens leaf


text: Institute Grassland & Environmental Res

text: live

text: Generic

text: Trifolium repens

text: root

text: Trifolium repens root


text: Institute Grassland & Environmental Res

text: live

text: Generic

text: Trifolium repens

text: leaf/root

text: Trifolium repens leaf/root


text: USDA-ARS

text: live

text: Generic

text: Source: Field collected from hibiscus June 2003 (Port St. Lucie, FL). A high quality EST with at least 200 contiguous bases at Trace Tuner score of 20 or better. Library construction by P.M. Dang (USDA-ARS-USHRL)

text: Mixed (nymphs - adults)

text: Maconellicoccus hirsutus

text: EcoRI

text: XhoI

text: Mixed sex

characteristic: sex

text: Whole body

text: WHMH (pink hibiscus mealybug)

text: pBluescript II SK+

characteristic: vector


characteristic: External Id

text: USDA-ARS-NAA Foreign Disease-Weed Science Research Unit

text: live

text: Generic

text: Williams

characteristic: cultivar

text: 1. Total RNA was isolated from infected soybean leaf tissue 13-15 days post inoculation with P. pachyrhizi isolate TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, TX), and the poly(A)+ mRNA was purified using the OLIGOTEX mRNA purification kit (Qiagen, Valencia, CA). 2. First strand cDNA was primed with a NotI primer-adapter (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. Double-stranded cDNA was ligated to Sal I adapter, digested with NotI and cloned into the pSPORT1 vector pre-cut with NotI and SalI. 4. The ligation mix was transformed into DH10B cells.

text: Glycine max

text: NotI

text: SalI

text: Leaf

characteristic: tissue-type

text: ABWZ Phakopsora pachyrhizi infected soybean leaf tissue 13-15 days post inoculation with TW72-1 urediniospores


characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: 1. Total RNA was isolated from germinating spores of P. pachyrhizi isolate TW 72-1 using the ToTally RNA kit (Ambion, Inc., Austin, TX), and the poly(A)+ mRNA was purified using the OLIGOTEX mRNA purification kit (Qiagen, Valencia, CA). 2. First strand cDNA was primed with a NotI primer-adapter (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. Double-stranded cDNA was ligated to Sal I adapter, digested with NotI and cloned into the pSPORT1 vector pre-cut with NotI and SalI. 4. The ligation mix was transformed into DH10B cells.

text: Germinating urediniospores

text: Phakopsora pachyrhizi

text: NotI

text: SalI

text: Taiwan 72-1

characteristic: strain

text: ACAC Phakopsora pachyrhizi TW72-1 germinating urediniospores on water surface (20C/16 hours/dark)


characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Williams

text: 1. Total RNA was isolated from infected soybean leaf tissue 6-8 days post inoculation with P. pachyrhizi isolate TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, TX), and the poly(A)+ mRNA was purified using the OLIGOTEX mRNA purification kit (Qiagen, Valencia, CA). 2. First strand cDNA was primed with a NotI primer-adapter (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. Double-stranded cDNA was ligated to Sal I adapter, digested with NotI and cloned into the pSPORT1 vector pre-cut with NotI and SalI. 4. The ligation mix was transformed into DH10B cells.

text: Glycine max

text: NotI

text: SalI

text: Leaf

text: ACBU Phakopsora pachyrhizi infected soybean leaf tissue 6-8 days post inoculation with TW72-1 urediniospores


text: live

text: Generic

characteristic: NCBI submission package

text: Phakopsora pachyrhizi TW72-1 urediniospores (collected from infected soybean plants, frozen in liquid nitrogen and kept at -80C). 1. Total RNA was isolated from urediniospores of P. pachyrhizi isolate TW 72-1 using the ToTally RNA kit (Ambion, Inc., Austin, TX), and the poly(A)+ mRNA was purified using the OLIGOTEX mRNA purification kit (Qiagen, Valencia, CA). 2. First strand cDNA was primed with a NotI primer-adapter (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. Double-stranded cDNA was ligated to Sal I adapter, digested with NotI and cloned into the pSPORT1 vector pre-cut with NotI and SalI. 4. The ligation mix was transformed into DH10B cells.

text: Urediniospores

text: Phakopsora pachyrhizi

characteristic: organism

text: NotI

text: SalI

text: Taiwan 72-1

text: CZAA Phakopsora pachyrhizi TW72-1 urediniospores


text: Louisiana State University Agricultural Center

text: live

text: Generic

text: Vermilion

text: Salinity induced EST identified by sequencing of complementary DNA library

characteristic: description

text: Vegetative

text: Sporobolus alterniflorus

text: Leaves

text: Spartina alterniflora leaf salinity induced expressed sequence tag (EST)

characteristic: title

text: pBluescript(r) II SK (+) (Stratagene, La Jolla, CA, USA)


text: live

text: Generic

text: Vermilion

text: Vegetative

characteristic: dev-stage

text: Sporobolus alterniflorus

text: Root

text: Spartina alterniflora root salinity induced expressed sequence tag (EST)


text: University of Texas Health Science Center at Houston

text: live

text: Generic

text: Zebrafish were purchased from a local pet store and maintained in the University of Texas Health Science Center fish facility. Corneas from 250 adult fish were dissected and collected for total RNA preparation. A corneal cDNA library was constructed using the Creator SMART cDNA library construction kit (Clontech, Mountain View, CA). Analyzed data available through http://neibank.nei.nih.gov.

text: adult

characteristic: lab-host

text: Eye

characteristic: organ

text: Danio rerio

text: Cornea

text: Zebrafish cornea

text: Plasmid

text: pDNR-Lib


text: University of Minnesota

text: live

text: Generic

text: Meleagris gallopavo

text: skeletal muscle

text: plasmid

characteristic: vector


characteristic: External Id

text: United States Dept of Agriculture

text: live

text: Generic

text: Hyphae isolated from 2, 4, 6, 8 dpi bean leaves

text: Phaseolus vulgaris

text: Uromyces appendiculatus

text: Race 41

text: pBluescript II SK+


text: Faculty of Science & Technology

text: live

text: Generic

text: Mycelial Growth

text: Colletotrichum gloeosporioides

text: Colletotrichum gloeosporioides Mycelia Grown in Crab Chitin


text: Faculty of Science & Technology

text: live

text: Generic

text: Mycelial Growth

text: Colletotrichum gloeosporioides

text: Colletotrichum gloeosporioides Mycelia Grown in Potato Dextrose Broth


text: ENH Research Institute, Feinberg School of Medicine, Northwestern University

text: live

text: Generic

text: HeLa

characteristic: cell-line

text: 3' EST from cytoplasmic mRNA of HeLa cell collected by 454 pyrosequencing system

text: Homo sapiens

text: Female

characteristic: sex

text: 3' ESTs from HeLa cell


text: Nihon University

characteristic: INSDC center name

text: live

text: Generic

text: immature

text: Oncorhynchus masou ishikawae

text: male

text: liver

characteristic: tissue-type

text: Oncorhynchus masou ishikawae liver immature


text: Washington University School of Medicine

characteristic: INSDC first public

text: live

text: Generic

text: Nippostrongylus brasiliensis

text: BsrgI

text: BsrgI

text: Plasmid


text: John Innes Centre

characteristic: INSDC last update

text: live

text: Generic

text: normalised cDNA library from the aerial tissues of N. benthamiana, in a small plasmid vector pGEM

text: Nicotiana benthamiana

text: Green Tissue

text: pGEM


text: Heilongjiang University

text: live

characteristic: INSDC status

text: Generic

text: Nodulisporium sylviforme

characteristic: strain

text: Specific Express Library for Biosynthesis of Taxol


text: Agriculture and Agri-Food Canada

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Excised library

text: Folded and unfolded leaves at tip of the shoot

text: E. coli SOLRTM (Strategene)

text: Leaves

text: Lotus uliginosus

text: Developing Leaf UL cDNA Library

text: phagemid

text: pBluescript SK(+/-)


text: Agriculture and Agri-Food Canada

text: live

text: Generic

text: Can be used for yeast two-hybrid screening

characteristic: description

text: E. coli DH10B(Strategene)

text: Leaves

text: Lotus uliginosus

characteristic: organism

text: Developing Leaf ULY cDNA Library

text: plasmid

text: pPC86 (Invitrogen)


text: China Agricultural University

text: live

text: Generic

text: adult

characteristic: dev-stage

text: Liver

text: Gallus gallus

text: Female

text: Silkie Fowl

text: Liver tissue

text: Chicken Liver cDNA Library

characteristic: title


text: INRA

text: live

text: Generic

text: Adult

text: Haemonchus contortus

text: male

text: Kokstad

text: Haemonchus contortus adult


text: Leiden/Amsterdam Center for Drug Research (LACDR)

text: live

text: Generic

text: adult

text: brain

characteristic: organ

text: Callithrix jacchus

text: male

text: hippocampus

text: Marmoset hippocampus normalized cDNA library


text: The Samuel Roberts Noble Foundation

text: live

text: Generic

text: Nicotiana benthamiana

text: N. benthamiana cDNA library


text: Murdoch University

text: live

text: Generic

text: Parastagonospora nodorum SN15

text: cDNA library of Phaeosphaeria nodorum grown on wheat cell walls


text: College of Veterinary Medicine

text: live

text: Generic

text: Total RNA was isolated from pools of adult females and males, separately, and suppression subtractive hybridization (SSH) was performed by using the PCR-Select cDNA Subtraction kit (Clontech): the forward subtracted library was constructed by using females as tester and males as driver, and the reverse-subtracted library was constructed by using males as tester and females as driver. Forward library was ligated into pGEM-T easy vector. Recombinant plasmids were transformed into competent Escherichia coli cells.

text: adult

text: Culex pipiens pallens

text: female

text: Shenzhen

text: sex-differentially expressed cDNA library for Culex pipiens pallens

text: Plasmid


text: USDA/ARS

text: live

text: Generic

text: The E. acervulina cDNA was cloned into the Uni-ZAP XR Vector (Stratagene) incorporating the EcoRI and XhoI cloning sites at the 5' and 3' end of the insert respectively. A mass in vivo excision was carried out producing a pBluescript SK (-) plasmid library (Stratagene). The effective library titer was approximately 8x106 cfu/ml. The excised library was propagated in the SOLR E. coli strain, and stocks were frozen in LB medium containing 15% glycerol. The library was constructed by Amplicon Express (Pullman, WA).

text: second generation merozoite and schizont

text: SOLR

characteristic: lab-host

text: Eimeria acervulina

text: EcoRI

text: XhoI

text: E. acervulina cDNA from second generation merozoites and schizonts

text: plasmid

text: pBluescript SK(-)

characteristic: vector


characteristic: External Id

text: The University of Florida College of Agriculture

text: live

text: Generic

text: Plasenta cell

characteristic: cell type

text: Cabb and Abott 5244

characteristic: cultivar

text: Ethylene induced gene expression

text: Full ripe

text: E. coli

text: Fruit

text: Citrullus lanatus

text: Plasenta

text: Watermelon gene expression

text: Plasmid

text: PCR-TRAP


text: Miyazaki University

characteristic: INSDC center name

text: live

text: Generic

text: adult

text: Ilyoplax pusilla

text: male

characteristic: sex

text: whole body

characteristic: tissue-type

text: Intertidal ocypodid crab whole body cDNA library


text: Ruder Boskovic Institute

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: The library contains 3'RACE products derived from PRAT satellite.

text: Palorus ratzeburgii


text: Ruder Boskovic Institute

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: The library contains 5'RACE products derived from PRAT satellite.

text: Palorus ratzeburgii


text: Ruder Boskovic Institute

text: live

text: Generic

text: The library contains directly sequenced PCR products and cloned PCR products. The cDNA obtained in primer extension reaction was used as a template for PCR.

characteristic: description

text: Palorus ratzeburgii

characteristic: organism

text: Palorus ratzeburgii PCR products


text: Murdoch University

text: live

text: Generic

text: Parastagonospora nodorum SN15

text: S. nodorum in planta cDNAs

characteristic: title


text: Murdoch University

text: live

text: Generic

text: Parastagonospora nodorum SN15

text: S. nodorum miscellaneous cDNAs


text: Universite catholique de Louvain (UCL)

text: live

text: Generic

text: country: Spain:Canaries Islands ; extra-radical mycelium produced in vitro after exposure to root signals ; isolation_source: plantation

text: 3 months-old

characteristic: dev-stage

text: Musa cavendish

text: Glomus sp. MUCL 41833

text: MUCL 41833 = DAOM 233750

characteristic: strain

text: mycelium

text: Glomus sp. MUCL 41833 mycelium 3 months-old


text: University of Helsinki

text: live

text: Generic

text: 84.194.30 times 2x(v-2)7

text: diploid potato lines

text: Solanum tuberosum

text: potato leaves

text: Solanum tuberosum potato leaves


text: Fujian Normal University

text: live

text: Generic

text: Schizochytrium sp. FJU-512

text: Schizochytrium cDNA library

text: pBluescript II SK(+)

characteristic: vector


characteristic: External Id

text: Institute of Genetics and Developmental Biology, Chinses Academy of Sciences

text: live

text: Generic

text: KingII

text: three-leaf-stage

text: E.coli DH5a

characteristic: lab-host

text: Secale cereale

text: leaf

text: Plasmid


text: USDA-ARS

text: live

text: Generic

characteristic: cultivar

text: The library was prepared using PolyA+ mRNA from root hairs isolated from dark-grown 8-day-old BTx623 seedlings. Double stranded cDNA was cloned unidirectionally into the XhoI (CTCGAG) and EcoRI (GAATTC) sites of the Uni-Zap XR vector using the Uni-Zap XR cDNA library construction kit (Stratagene, La Jolla, CA). Plant material and RNA were prepared by Dr. Scott Baerson (USDA-ARS, Natural Products Utilization Research Unit).

text: SOLR strain of E. coli

text: Sorghum bicolor

text: XhoI

text: EcoRI

text: Sorghum bicolor BTx623 Root hair

text: Plasmid

text: pBlueScriptSK-


characteristic: INSDC center name

text: live

text: Generic

text: KingII

text: seedling three-leaf-stage

text: E.coli DH5a

text: Secale cereale

text: leaf

text: rye_KingII_chromsome1R_Salicylic Acid induced_ESTs_seedlings

text: Plasmid


text: Huazhong Agricultural University

text: live

text: Generic

text: skeletal muscle

characteristic: cell type

text: The cDNA 3' sequences were obtained from GLGI (generation of longer cDNA fragments from serial analysis of gene expression tags for gene identification) experiments

text: embryonic day 65 post-fertilization

text: Sus scrofa

text: Male and female

characteristic: sex

text: Tongcheng pig

text: longissimus muscle

text: Sus LongSAGE/GLGI Express Library


text: USDA-ARS, Center for Medical, Agricultural and Veterinary Entomology

characteristic: INSDC first public

text: live

text: Generic

text: Total RNA was extracted from 22.5 grams of all stages of a Solenopsis invicta colony with guanidinium isothiocyanate. The RNA was DNAse treated and extracted with phenol:chloroform:isoamyl alcohol. mRNA was subsequently purified with the MicroPoly(A) Pure RNA extraction protocol (Ambion, Austin, TX). The library was created following the ZAP-cDNA Synthesis kit (Stratagene, La Jolla, CA).

text: egg, larva, worker, pupa, queen

text: Solenopsis invicta

text: EcoR I

text: Xho I

text: Male and female

text: Monogyne

text: Whole organism

characteristic: tissue-type

text: Fire ant Uni-ZAP XR Library

text: Phage

text: Uni-ZAP XR


text: University of Minnesota, Duluth

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: From the brainstem of hibernating 13-lined ground squirrels, 3 male and 3 female. Taken in December, with a lab temperature of 5 C. Animals were housed in complete darkness during that time, with no food but water ad libitum.

text: Adult

text: E. coli

text: Brainstem

characteristic: organ

text: Ictidomys tridecemlineatus

text: Not I

text: Sal I

text: Hibernating 13-lined squirrel brainstem library

text: Plasmid


text: Agriculture & Agri-Food Canada

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: E. coli DH5alpha

text: Brassica oleracea var. alboglabra

text: SalI

text: NotI

text: Young, small leaves off 3-week old plant

text: Brassica oleracea var. alboglabra leaf cDNA library

text: plasmid


text: Trinity University

text: live

text: Generic

text: eCG/hCG-primed immature rat

text: Rattus norvegicus

characteristic: organism

text: W

text: ovary

text: eCG/hCG-primed immature rat ovary

characteristic: title


text: Inra

text: live

text: Generic

text: Gallus gallus

text: Gallus gallus library (Leroux S)


text: Departement de Biochimie, Universite de Montreal

text: live

text: Generic

text: Neocallimastix patriciarum

text: Neocallimastix patriciarum ZAP II cDNA library


text: Genome Institute of Singapore

text: live

text: Generic

text: Priming method: GsuI-d(T) priming ; Priming sequence: 5' -GAGCTAGTTCTGGAG(T)16VN -3' ; Directionally cloned: ; 5' cloning site: NotI site GCGGCCGC ; 5' linker/adaptor sequence: 5' -GGATCCGAC -3' ; 3' cloning site: BseRI ; Insert size range: 2kbp-700bp ; For PCR insert analysis: M13 forward and reverse primers ; Library is not amplified ; Recombinants (inserts): 100% ; Library complexity: 1.6 X 106 cfu ; Full-length construction (method): Ng et al, Nature Method 2005 2(2):105-111 ; The pooled tissue RNA was collected and used to construct full length enriched cDNA library and also served as template to synthesize complex first strand cDNA probe. Two high density colony arrays were made from over 110K cDNA clones and hybridized with the probes. Low abundant clones were selected by the Colony Array Subtractive Hybridization (CASH) analysis as they represented rare expressed clones. The hybridization intensities for all clones span from 0 to 200,000 counts and the low abundant selected clones in this library ranges from 0 to 28,000.

characteristic: description

text: Adult fish of 3-4 months old

characteristic: dev-stage

text: Escherichia Top10

text: Ovary

text: Danio rerio

text: BseRI

text: Not I

text: Females only

text: Tubingen (TU)

characteristic: strain

text: Ovary

text: Plasmid


characteristic: External Id

text: Genome Institute of Singapore

text: live

text: Generic

text: Priming method: GsuI-d(T) priming ; Priming sequence: 5' -GAGCTAGTTCTGGAG(T)16VN -3' ; Directionally cloned: ; 5' cloning site: NotI site GCGGCCGC ; 5' linker/adaptor sequence: 5' -GGATCCGAC -3' ; 3' cloning site: BseRI ; Insert size range: 2kbp-750bp ; For PCR insert analysis: M13 forward and reverse primers ; Library is not amplified ; Recombinants (inserts): 92% ; Library complexity: 1.3 X 106 cfu ; Full-length construction (method): Ng et al, Nature Method 2005 2(2):105-111 ; The pooled tissue RNA was collected and used to construct full length enriched cDNA library and also served as template to synthesize complex first strand cDNA probe. Two high density colony arrays were made from over 110K cDNA clones and hybridized with the probes. Low abundant clones were selected by the Colony Array Subtractive Hybridization (CASH) analysis as they represented rare expressed clones. The hybridization intensities for all clones span from 0 to 1 million counts and the low abundant selected clones in this library ranges from 0 to 17,000.

text: 0-72 hours post fertilization embryos

text: Escherichia Top10

characteristic: lab-host

text: Embryos

text: Danio rerio

text: BseRI

text: Not I

text: Male and female embryos mixed

text: Tubingen (TU)

text: Embryos

text: Plasmid

characteristic: vector


text: Henry Ford Health System

characteristic: INSDC center name

text: live

text: Generic

text: Differentially expressed mRNA transcripts identified by RT-PCR differential display (DD) analysis of RNA isolated from tumor and patient-matched non-tumor prostate tissues.

text: Prostate

text: Homo sapiens

text: Male

text: Prostate

text: mRNA markers for prostate cancer detection


text: Animal Science

text: live

text: Generic

text: Xiao Guan

characteristic: cultivar

text: Differential expressed sequences cloned to pGEM T-easy vector and transformed to the E.coli JM109 competent cell

text: 4 day old seedling

text: JM109 E.coli

text: Medicago sativa

text: EcoR

text: Spe

text: root

text: Alfalfa aluminum suppression subtractive library

text: Plasmid

text: promega pGEM T-easy


text: South Dakota State University

characteristic: INSDC first public

text: live

text: Generic

text: Sequence expressed uniformly in endodormant and paradormant buds. Source tissue from GRIN accession PI 588259 or another V. riparia collected in the same location.

text: paradormant

text: bud

text: Vitis riparia

text: SalI

text: SacII

text: Vitis riparia paradormant differential display fragment

text: pGem


text: South Dakota State University

text: live

text: Generic

text: Source tissue from GRIN accession PI 588259 or another V. riparia collected in the same location.

text: endodormant

text: bud

text: Vitis riparia

text: Vitis riparia endodormant differential display fragment

text: Novagen


text: South Dakota State University

text: live

text: Generic

text: VRJ is a cDNA library of Vitis riparia endodormant buds. Endodormant buds were collected from insect and disease free vines that were induced into dormancy with short photoperiods. The directionally oriented library was constructed according to Gibco BRL Superscript Plasmid System for cDNA synthesis and plasmid cloning. Source tissue from GRIN accession PI 588259 or another V. riparia collected in the same location.

text: dormant

text: bud

text: Vitis riparia

text: SalI

text: NotI

text: Vitis riparia endodormant bud - VRJ

text: pSport 1


text: The Institute of Physical and Chemical Research (RIKEN)

text: live

text: Generic

text: 1 cell

characteristic: cell type

text: 1 cell embryo

text: Mus musculus

text: RIKEN full-length enriched mouse cDNA library, C57BL/6J 1 cell embryo


characteristic: INSDC last update

text: live

text: Generic

text: morula

text: Mus musculus

text: whole body

characteristic: tissue-type

text: RIKEN full-length enriched mouse cDNA library, C57BL/6J whole body morula


text: Center for the Biology of Nematode Parasitism, North Carolina State University

text: live

characteristic: INSDC status

text: Generic

text: Hicks Broadleaf

text: cold stressed

text: Nicotiana tabacum

text: Plasmid


text: University of Delhi South Campus

text: live

text: Generic

characteristic: NCBI submission model

text: Library was constructed from total RNA using Clontech SMART cDNA library construction kit following manufacturerÆs instructions

text: Mixed

text: E.coli DH5alfa

text: Polypleurum stylosum

text: SfiI

text: SfiI

text: Whole-plant

text: Polypleurum whole-plant mixed library

text: Plasmid

text: pDNR-LIB


text: Bhabha Atomic Research Center

text: live

text: Generic

characteristic: NCBI submission package

text: Lambda Zap Express Library

text: Trichoderma virens

text: Mycelia

text: Trichoderma virens IMI 304061 cDNA library


text: Auburn University

text: live

text: Generic

text: Crassostrea virginica

characteristic: organism

text: Sfi IA

text: Sfi IB

text: Mixed tissue

text: Oyster mixed tissue normalized cDNA library

text: Phagemid

text: pDNR-LIB


text: School of Radiation Medicine and Public Health, Suzhou University

text: live

text: Generic

text: Mus musculus

text: Male

characteristic: sex

text: BALB/c

text: Bone Marrow

text: Mus musculus bone marrow Express Library

characteristic: title


text: Universidade Federal de Goias

text: live

text: Generic

text: Paracoccidioides brasiliensis

text: Paracoccidioides brasiliensis pCMV-SPORT 6 library

text: Plasmid


text: USDA-ARS

text: live

text: Generic

text: Greenhouse-reared first generation progeny of G. atropunctata collected in Sonoma Co., CA. A high quality EST with at least 150 contiguous bases at Trace Tuner score of 20 or better. Library construction by L.E. Hunnicutt (USDA-ARS-USHRL).

characteristic: description

text: Adult

characteristic: dev-stage

text: Graphocephala atropunctata

text: EcoRI

text: XhoI

text: Mixed

text: Whole body

text: Adult Blue-green Sharpshooter (WHGA)

text: pBluescript II SK+


characteristic: External Id

text: National Agricultural Research Center for Hokkaido Region

text: live

text: Generic

text: Lee

text: Glycine max

text: roots infected by soybean cyst nematode, Heterodera glycines

text: Glycine max cv. Lee roots infected by soybean cyst nematode


text: live

text: Generic

text: Pickett 71

text: Glycine max

text: Glycine max cv. Pickett 71 roots infected by soybean cyst nematode


text: live

text: Generic

text: Heterodera glycines

text: Race 3

characteristic: strain

text: Heterodera glycines Race 3


text: live

text: Generic

characteristic: lab-host

text: Mus musculus

text: Male

text: BALB/c

text: Peripheral blood

text: Mus musculus Peripheral blood Express Library


text: National Institute of Agricultural Science and Technology, RDA

characteristic: INSDC center name

text: live

text: Generic

text: 5 instar

text: Spodoptera litura

text: whole body

text: Spodoptera litura oxidative-stress responsive cDNA library


text: University of Agricultural Sciences

text: live

text: Generic

characteristic: cultivar

text: 15-day-old Mulberry seedlings were subjected to different levels of moisture stress (70%FC, 50%FC and 100%FC) by gradually withholding water till the plants withstand the respective stress level. RNA was isolated from the leaf tissues and subtracted cDNA library was constructed using T/A cloning kit.

text: 20 day-old seedlings

text: E. coli

text: Morus alba

text: Eco RI

text: Leaf

text: Mulberry dehydration stress subtracted cDNA library

text: TA cloning vector

characteristic: vector


text: IRD (Institut de Recherche pour le Developpement)

characteristic: INSDC first public

text: live

text: Generic

text: Laurina

text: Clontech PCR-select cDNA subtraction library; Laurina-specific (Laurina as tester and Bourbon as driver in the subtraction process)

text: E.coli

text: leaf

characteristic: organ

text: Coffea arabica

text: Laurina subtractive library SSH1

text: PGEM-T


characteristic: INSDC last update

text: live

text: Generic

text: Bourbon

text: Clontech PCR-select cDNA subtraction library; Bourbon-specific (Bourbon as tester and Laurina as driver in the subtraction process)

text: E.coli

text: leaf

text: Coffea arabica

text: Bourbon subtractive library SSH2

text: PGEM-T


text: live

characteristic: INSDC status

text: Generic

text: Medicago truncatula

text: Turf and forage library


text: Faculty of Horticulture, Chiba University

text: live

text: Generic

characteristic: NCBI submission model

text: Chinese Spring

text: Triticum aestivum

text: pistil

characteristic: tissue-type

text: cDNA-AFLP screen, Chinese Spring


text: Dept. Biology and C.R.I.B.I.-Biotechnology Centre, University of Padova

text: live

text: Generic

characteristic: NCBI submission package

text: RNA was isolated from mussel haemocytes after treatment with inactivated cocktail of Vibrio sp. The cDNA was synthesized from mRNA with a modified SMART approach (Clontech). The oligos were designed with attB-sequences allowing size-selected cDNA cloning by Gateway Cloning technology (Invitrogen).

text: E.coli

text: Mytilus galloprovincialis

characteristic: organism

text: ND

text: Haemolymph

text: plasmid

text: pDONR221 (Invitrogen)


text: live

text: Generic

text: RNA was isolated from mussel haemocytes after treatment with PolyI:C. The cDNA was synthesized from mRNA with a modified SMART approach (Clontech). The oligos were designed with attB-sequences allowing size-selected cDNA cloning by Gateway Cloning technology (Invitrogen).

text: E.coli

text: Mytilus galloprovincialis

text: ND

characteristic: sex

text: Haemolymph

characteristic: title

text: plasmid

text: pDONR221 (Invitrogen)


text: CSIC

text: live

text: Generic

text: cDNA was synthesized from 1 ug of each haemolymph RNA sample (bacterial infected and control) with the SMART PCR cDNA Synthesis Kit (Clontech). A SSH assay was then performed using the PCR-Select cDNA Subtraction Kit (Clontech) following manufacturer's instruction

characteristic: description

text: E.coli

text: Mytilus galloprovincialis

text: ND

text: Haemolymph

text: plasmid

text: pCR2.1TOPO (Invitrogen)


characteristic: External Id

text: CSIC

text: live

text: Generic

text: cDNA was synthesized from 1 ug of each haemolymph RNA sample (poly I:C stimulated and control) with the SMART PCR cDNA Synthesis Kit (Clontech). A SSH assay was then performed using the PCR-Select cDNA Subtraction Kit (Clontech) following manufacturer's instruction

text: E.coli

text: Mytilus galloprovincialis

text: ND

text: Haemolymph

text: plasmid

text: pCR2.1TOPO (Invitrogen)


text: Royal Holloway, University of London

text: live

text: Generic

text: Petit Havana

text: Tobacco suspension cell culture transformed with a constitutively expressed Agrobacterium derived tcyt gene

text: Third day after subculturing

characteristic: dev-stage

text: Nicotiana tabacum

text: Transformed tobacco Lambda Zap II library

text: Lambda Zap II UniZAP-XR


text: live

text: Generic

text: Total RNA was extracted from whole organisms, checked for quality, then prepared for construction and normalization of the cDNA library by Invitrogen. Plasmids were isolated and inserts end-sequenced by the Marine DNA Sequencing and Analysis Facility at the Center for Marine Functional Genomic Studies, Mount Desert Island Biological Laboratory (J.N. Forrest, Jr., Director). Traces were processed for submission to dbEST by trace2dbest software (Parkinson, Anthony and Blaxter, unpublished software). The project was supported by NIH Grant Number P20-RR-16463 from the INBRE Program of the National Center for Research Resources (P.H. Hand, PI).

text: C5 juveniles and adults

text: Calanus finmarchicus

text: Copepod Whole Organism, Normalized

text: pCMV Sport 6.1


text: NI Vavilov Institute of General Genetics, Russian Academy of Sciences

text: live

text: Generic

text: A Sequence-Indexed Library of Insertion Mutations in the Arabidopsis Genome

text: 4 weeks old plant

text: X-line

characteristic: isolate

text: leaf

text: Arabidopsis thaliana

text: hermaphrodite

text: Arabidopsis thaliana X-line TDNA insertion lines PCR

text: overexpression vector

text: pFC12850OE(GenBank ID: DQ995486)


text: Universita degli Studi dell'Insubria

characteristic: INSDC center name

text: live

text: Generic

text: adult

text: Thunnus thynnus

text: liver

text: T. thynnus adult liver cDNA expression library


text: UNESP Campus de Ilha Solteira

text: live

text: Generic

text: Differentially expressed genes in Citrus apomitic embryos (subtractive cDNA library)

text: apomitic

text: Citrus sinensis

text: seed


text: UNESP Campus de Ilha Solteira

text: live

text: Generic

text: Differentially expressed genes in Citrus zygotic embryos (subtractive cDNA library)

text: zygotic

text: Citrus sinensis

text: seed


text: live

text: Generic

characteristic: cultivar

text: 4 weeks old plant

text: O-line

text: leaf

text: Arabidopsis thaliana

text: hermaphrodite

text: Arabidopsis thaliana O-line TDNA insertion lines PCR

text: overexpression vector

text: pFC12860OE(GenBank ID: DQ995487)


text: School of Radiation Medicine and Public Health, Suzhou University

text: live

text: Generic

characteristic: lab-host

text: Rattus norvegicus

text: Male

text: Wistar

characteristic: strain

text: pulmonary epithelial

text: Rat pulmonary epithelial Express Library


text: US Army Engineer Research and Development Center-Environmental Laboratory

characteristic: INSDC first public

text: live

text: Generic

text: Two cDNA libraries were constructed using the SSH technique. The first SSH library was made using pooled mRNA (10 microgram) extracted from control unexposed worms against worms exposed to Cd (2.6 mmol/kg), TNT (100 mg/kg), 2,6-DNT (54 mg/kg), RDX (50 mg/kg), or HMX (10 mg/kg), whereas mRNA (10 microgram) from worms exposed to Cu (293 mg/kg), Pb (8778 mg/kg), Zn (357 mg/kg), 2,4-DNT (100 mg/kg), and TNB (100 mg/kg) was run against mRNA from another set of control worm for the second library. Exposure (4-, 14-, or 28-d) was conducted in an Organization for Economic Cooperation and Development (OECD) artificial soil consisting of 70% sand, 20% kaolin clay, and 10% 2-mm sieved peat moss with an adjusted pH between 6.5 and 7.0. Exposed and unexposed earthworms were fixed in RNAlater (Ambion) and stored at -80oC. Total RNA was extracted using RNeasy kit (Qiagen), and poly(A) mRNA was purified using NucleoTrap mRNA purification kit (BD Biosciences). The Clontech PCR-Select cDNA subtraction kit (BD Biosciences) was then used to enrich for differentially expressed genes.

text: adults

text: Eisenia fetida

text: whole worm

text: Earthworm SSH cDNA library


text: University of California at Davis (UCD)

characteristic: INSDC last update

text: live

text: Generic

text: The normalized library was constructed from multiple sources of RNA from a single genotype using Trimmer-Direct kit. cDNAs were then size-fractionated, directionally cloned into a custom medium-copy vector and transformations made with three size classes (L-large, M-medium and S-small) to minimize size bias due to differential transformation efficiency. Details of each source of RNA and library construction can be obtained at http://cgpdb.ucdavis.edu/

text: E.coli

text: Cichorium intybus

text: CCI(LMS) chicory

text: Plasmid

text: pBRcDNASfiAB

characteristic: vector


text: University of California at Davis (UCD)

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: E.coli

text: Centaurea maculosa

text: CNM(LMS) spotted knapweed

text: Plasmid

text: pBRcDNASfiAB


text: University of California at Davis (UCD)

text: live

text: Generic

characteristic: NCBI submission package

text: E.coli

text: Centaurea solstitialis

text: CNS(LMS) yellow starthistle

text: Plasmid

text: pBRcDNASfiAB


text: Chinese Academy of Agricultural Sciences

text: live

text: Generic

text: Medicago truncatula

characteristic: organism

text: Medicago truncatula Subtractive PCR


text: University of Alberta

text: live

text: Generic

text: Norlin

text: Plants were raised in growth chambers with a photoperiod of sixteen hours light and eight hours dark at 24oC and 50% humidity. At the start of the fourth-week following sowing, the outside of the stem was scraped was harvested for RNA extraction. cDNA synthesis was primed using a NotI-oligodT primer, and using SalI adapters, double-stranded DNA was ligated into SalI NotI sites of pCMV-SPORT6.

text: 4 weeks post-sowing

text: stem

characteristic: organ

text: Linum usitatissimum

text: SalI

text: NotI

text: cortical regions including cambium, phloem, bast fibers

characteristic: tissue-type

text: stem phloem (bast) fiber enriched library LU01

text: pCMV-SPORT 6


text: University of Bonn

text: live

text: Generic

text: embryo day 14,21; fetus day 35,49,63,77,91

text: Sus scrofa

text: fetal musculus longissimus dorsi

text: Group5 EST from DDRT-PCR analysis of myogenesis

characteristic: title


characteristic: External Id

text: University of Bonn

text: live

text: Generic

text: embryo day 14,21

text: Sus scrofa

text: Group1 EST from DDRT-PCR analysis of myogenesis


text: University of Bonn

text: live

text: Generic

text: embryo day 14-21; fetus day 35,49

characteristic: dev-stage

text: Sus scrofa

text: fetal musculus longissimus dorsi

text: Group2 EST from DDRT-PCR analysis of myogenesis


text: University of Bonn

text: live

text: Generic

text: fetus day 49,63,77,91

text: Sus scrofa

text: fetal musculus longissimus dorsi

text: Group3 EST from DDRT-PCR analysis of myogenesis


text: University of Bonn

text: live

text: Generic

text: embryo day 21; fetus day 35,49,63,77,91

text: Sus scrofa

text: fetal musculus longissimus dorsi

text: Group4 EST from DDRT-PCR analysis of myogenesis


text: Tokyo University of Agriculture

text: live

text: Generic

text: blastocyst

text: Mus musculus

text: ES clone subtracted cDNA library


text: Michigan State University

text: live

text: Generic

text: Arabidopsis seedlings grown on soil 8-days in continuous light at 20 C. RNA was extracted from aerial tissues and cDNA prepared from mRNA after oligodT priming.

characteristic: description

text: 8 day seedlings

text: Arabidopsis thaliana

text: aerial

text: 8-day Arabidopsis seedlings, aerial tissues


text: Universitat Autonoma de Barcelona, Facultat de Veterinaria

characteristic: INSDC center name

text: live

text: Generic

text: Pregnant: 62h post-mating

text: Oviduct

text: Oryctolagus cuniculus

text: Female

characteristic: sex

text: Rabbit oviductal cDNA library

text: Plasmid

text: pDNR-LIB


text: live

text: Generic

text: 5h post-mating

text: ovary

text: Oryctolagus cuniculus

text: Female

text: Rabbit ovarian cDNA library

text: Plasmid

text: pDNR-LIB


text: live

text: Generic

text: Pregnant: 5 days post-mating

text: uterus

text: Oryctolagus cuniculus

text: Female

text: Rabbit uterine cDNA library

text: Plasmid

text: pDNR-LIB


text: CSIRO Entomology

text: live

text: Generic

text: A toadlet cDNA library was constructed with the plasmid pCMVSport6 and gene fragments PCR amplified using M13/pUC forward and reverse primers.

text: Toadlet

text: Rhinella marina

text: Toadlet cDNA Library

text: Plasmid


text: Central Institute of Medicinal and Aromatic Plants

text: live

text: Generic

text: secretory cell

characteristic: cell type

text: Oil gland cDNA library was constructed in Lambda Zap Express vector (Stratagene) which was in vivo excised to generate phagemid pBK-CMV vector containing transcript messages from peltate glandular trichomes of Mentha arvensis L.

text: 90 days after planting

text: E. coli XL1-Blue MRF'strain and XLOLR strain

characteristic: lab-host

text: Leaf

text: Mentha canadensis

text: XhoI

text: EcoRI

text: peltate glandular trichome

text: Mentha arvensis trichome specific cDNA library in Lambda Zap Express vector (Stratagene)

text: Phagemid

text: pBK-CMV


text: University of California at Davis (UCD)

characteristic: INSDC first public

text: live

text: Generic

text: The normalized library was constructed from multiple sources of RNA from a single genotype using Trimmer-Direct kit. cDNAs were then size-fractionated, directionally cloned into a custom medium-copy vector and transformations made with three size classes (L-large, M-medium and S-small) to minimize size bias due to differential transformation efficiency. Details of each source of RNA and library construction can be obtained at http://cgpdb.ucdavis.edu/

text: E.coli

text: Cichorium endivia

text: CCE(LMS) endive

text: Plasmid

text: pBRcDNASfiAB


text: University of California at Davis (UCD)

characteristic: INSDC last update

text: live

text: Generic

text: E.coli

text: Carthamus tinctorius

text: CFF(LMS) safflower

text: Plasmid

text: pBRcDNASfiAB


text: University of California at Davis (UCD)

text: live

text: Generic

text: E.coli

text: Helianthus ciliaris

text: CHC(LMS) Texas blueweed

text: Plasmid

text: pBRcDNASfiAB

characteristic: vector


text: University of California at Davis (UCD)

text: live

characteristic: INSDC status

text: Generic

text: E.coli

text: Helianthus tuberosus

text: CHT(LMS) Jerusalem artichoke

text: Plasmid

text: pBRcDNASfiAB


text: University of California at Davis (UCD)

text: live

text: Generic

characteristic: NCBI submission model

text: E.coli

text: Helianthus paradoxus

text: CHU(LMS) puzzle sunflower

text: Plasmid

text: pBRcDNASfiAB


text: Centre National de la Recherche Scientifique

text: live

text: Generic

characteristic: NCBI submission package

characteristic: cultivar

text: roots from 4 days stressed plants in vitro (150 mM NaCl)

text: ISV-CNRS

text: Roots

text: Medicago truncatula

text: Medicago truncatula cv. 108-R Salt-stress SSH

text: cloning vector

text: pGMTeasy


text: The Institute of Physical and Chemical Research (RIKEN)

text: live

text: Generic

text: 11 days embryo

text: Mus musculus

characteristic: organism

characteristic: strain

text: embryonic body

text: RIKEN full-length enriched mouse cDNA library, C57BL/6J embryonic body between diaphragm region and neck, 11 days embryo


text: live

text: Generic

text: 12 days embryo

text: Mus musculus

text: embryonic body

characteristic: tissue-type

text: RIKEN full-length enriched mouse cDNA library, C57BL/6J embryonic body between diaphragm region and neck, 12 days embryo

characteristic: title


characteristic: External Id

text: University of California at Davis (UCD)

text: live

text: Generic

text: The standard library was constructed from multiple sources of RNA from a single genotype. cDNAs were then size-fractionated, directionally cloned into a custom medium-copy vector and transformations made with three size classes (X-large,Y-medium and Z-small) to minimize size bias due to differential transformation efficiency. Details of each source of RNA and library construction can be obtained at http://cgpdb.ucdavis.edu/

text: E.coli

text: Carthamus tinctorius

text: CFF(XYZ) safflower

text: Plasmid

text: pBRcDNASfiAB


text: University of California at Davis (UCD)

text: live

text: Generic

characteristic: description

text: E.coli

text: Helianthus exilis

text: Ames 27246

text: CHE(XYZ) serpentine sunflower

text: Plasmid

text: pBRcDNASfiAB


text: University of California at Davis (UCD)

characteristic: INSDC center name

text: live

text: Generic

text: E.coli

text: Helianthus tuberosus

text: CHT(XYZ) Jerusalem artichoke

text: Plasmid

text: pBRcDNASfiAB


text: University of California at Davis (UCD)

text: live

text: Generic

text: E.coli

text: Helianthus paradoxus

text: CHU(XYZ) puzzle sunflower

text: Plasmid

text: pBRcDNASfiAB


text: University of California at Davis (UCD)

text: live

text: Generic

text: E.coli

text: Helianthus argophyllus

text: PI 494573

text: CHW(XYZ) silverleaf sunflower

text: Plasmid

text: pBRcDNASfiAB


text: University of SFAX, National Institute for Engineering Studies

text: live

text: Generic

text: Baklouti

text: Seeds were germinated in MS medium for 5 days. Seeds RNAs were extracted and used in RT-PCR. RT-PCR fragment was isolated and cloned in pSK+ vector.

text: five days germinated seeds

characteristic: dev-stage

text: Capsicum annuum

text: BamHI

text: SacI

text: seed

text: Capsicum annuum L. partial CDK library

text: Plasmid

text: pBluescriptSK+ vector


text: University of Connecticut Health Center

text: live

text: Generic

text: cDNA library from the synganglia of feeding adult female Ixodes scapularis ticks

text: adult

text: synganglia

characteristic: organ

text: Ixodes scapularis

text: SfiIA

text: SfiIB

text: female

characteristic: sex

text: Tick synganglia SMART cDNA Library

text: lambda phage


text: University of Victoria

characteristic: INSDC first public

text: live

text: Generic

text: Tissue contributors: Vanya Ewart (NRC Institute for Marine Biosciences, Halifax, N.S.)

text: brain

text: Osmerus mordax

text: omorobrh Osmerus mordax cDNA

text: pBlueScriptIISK+


text: Universidade Federal de Pernambuco

characteristic: INSDC last update

text: live

text: Generic

text: cDNA library from a 12h ectomycorrhizal interaction between P. tinctorius and C. sativa

text: Pisolithus tinctorius


text: Melaka Institute of Biotechnology

text: live

text: Generic

text: Somatic

characteristic: cell type

text: oleifera (Malaysian)

text: The cDNA library was prepared following all the guidelines and the protocols provided by manufacturer (Invitrogen Corporation) of the CloneMiner cDNA Library Construction Kit. This CloneMiner cDNA Library Construction Kit was used because it enables us to construct highly representative cDNA libraries without the risk of restriction enzymes cutting our precious cDNA clones. Therefore, we can isolate fully intact clones from high-quality cDNA library.

text: 17 week after anthesis (WAA)

text: Fruit

text: Elaeis oleifera

text: BsrGI

text: BsrGI

text: Mesocarp

text: Oil palm mesocarp-tissue cDNA Entry Library

text: Plasmid

text: Donor Vector, pDONR 222

characteristic: vector


text: Faculty of Agriculture and Natural Science, University of Pretoria

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Tugela

text: Transcript-derived fragments generated via cDNA-AFLP of wheat leaf mRNA after induction of resistance response to the Russian wheat aphid, Diuraphis noxia.

text: 2-3 leaf stage

text: Eschericia coli (DH5z)

characteristic: lab-host

text: leaf

text: Triticum aestivum

text: EcoRI

text: EcoRI

text: cDNA-AFLP of wheat response to Diuraphis noxia

text: plasmid

text: pGem-T Easy


text: Nanjing Medical University

text: live

text: Generic

characteristic: NCBI submission package

text: The cDNA clones were choosed from the subtractive library constructed by using supressive subtractive hybridization method. The mRNAs were isolated from hepatocellular carcinoma and the corresponding non cancer tissues.

text: hepatocellular carcinoma

text: Homo sapiens

characteristic: organism

text: male

text: liver

text: subtractive library of hepatocellular carcinoma

text: plasmid


text: Heinrich-Heine Universitaet Duesseldorf

text: live

text: Generic

text: Euglena gracilis

text: SAG 1224-5/25

characteristic: strain

text: Euglena gracilis strain SAG 1224-5/25


text: Boston University, Bioinformatics Program

text: live

text: Generic

characteristic: cell-line

text: Homo sapiens

text: acute promyelocytic leukemia

characteristic: tissue-type

text: Novel Promoters 5' RACE-PCR

characteristic: title

text: TOPO XL PCR cloning vector


characteristic: External Id

text: National Centre for Plant Genome Research

characteristic: INSDC center name

text: live

text: Generic

characteristic: cultivar

text: Suppression Subtractive Hybridisation

characteristic: description

text: Cicer arietinum

text: Leaf

text: Chickpea Insect-Induced Library


text: Indian Institute of Technology, Kharagpur

characteristic: INSDC first public

text: live

text: Generic

text: JRC 321

text: Total RNA was isolated from fibre forming stage of jute stem by hot borate method and purified by isopycnic CsCl density gradient ultracentrifugation. Poly (A+) mRNA was isolated using oligo (dT) cellulose. The cDNA was synthesized from the Poly (A+) mRNA using oligo (dT) 12-18 primer with the help of cDNA synthesis Time saver TM kit (Amersham) following the instruction of the manufacturer. The double stranded cDNA synthesized was size fractionated using Sephacryl S-300. The double stranded cDNA was then ligated to the EcoRI/NotI adapter, followed by kinasing and purification by Sephadex G50 column. The purified cDNA was ligated to EcoRI digested and Shrimp alkaline phosphatase treated pUC18 plasmid vector. Finally, the ligated product was transformed in E.coli DH10B competent cells. The library was hybridized with double stranded cDNA probe generated from poly(A)+mRNA of non-fibre forming stage of jute stem and unhybridized clones, which are expected to be involved in bast fibre development along with other developmental processes, were selected. The selected clones were then subjected to single pass sequencing. The resultant sequences were edited to remove the vector sequence. The clones were putatively identified by searching against plant database in TIGR using tblastx.

text: 45 days after sowing

characteristic: dev-stage

text: E.coli DH10B

text: Stem

characteristic: organ

text: Corchorus capsularis

text: EcoRI

text: EcoRI

text: Hermaphrodite

characteristic: sex

text: Bast fiber and associated tissues

text: Jute Plasmid library

text: Plasmid


text: The Plant Biotechnology Institute, National Research Council Canada

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Topas (embryogenic line DH4079)

text: RNA was extracted from microspores at the late-uninucleate and early-binucleate stages. The microspore donor plants were grown at 10 degrees C/5 degrees C (day/night)

text: Freshly-isolated late-uninucleate and early-binucleate microspores

text: Brassica napus

text: EcoR I

text: Xho I

text: Microspore

text: plasmid

characteristic: vector


text: Texas A & M University, College Station

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: cDNA pool of more than 5 weeks old adult female ticks that had attained appetence and exposed to host for 7 hours was subtracted against cDNA pool of newly molted adult ticks showing no desire to feed. Differential expression of cDNAs was confirmed by cDNA dot blot hybridization.

text: unfed adults

text: Amblyomma americanum

text: female

text: whole tick

text: Genes up regulated in Amblyomma americanum prior to host skin penetration


text: CNRS - Toulouse III University (IFR 30, INSERM CNRS Toulouse III University - CHU)

text: live

text: Generic

text: Keratinocyte

characteristic: cell type

text: This sequence was obtained by production of EST minilibraries using the ORESTES method, from ex vivo normal human epidermis samples enriched in granular keratinocytes

text: Skin

text: Homo sapiens

characteristic: organism

text: Epidermis

text: Human epidermis granular keratinocytes


text: Kochi University

text: live

text: Generic

text: Samsun NN

text: differential display

text: Nicotiana tabacum

text: Nicotiana tabacum Samsun NN gene responsive to Ralstonia solanacearum infection


text: Kochi University

text: live

text: Generic

text: differential display

text: Nicotiana tabacum

text: Nicotiana tabacum gene responsive to Ralstonia solanacearum infection


text: Australian Institute of Marine Science

text: live

text: Generic

text: libraries were prepared using the SMART cDNA Library Construction Kit (Clontech).

characteristic: lab-host

text: Penaeus monodon

text: hemocytes

characteristic: tissue-type

text: P. monodon hemocyte library

text: plasmid


characteristic: External Id

text: Warsaw University of Life Sciences, Faculty of Agriculture and Biology

text: live

text: Generic

text: Triada

text: tuber

text: Solanum tuberosum

text: Solanum tuberosum cv. Triada cDNA fragments

characteristic: title


text: University of Puerto Rico

characteristic: INSDC center name

text: live

text: Generic

text: Mixed species/subspecies: Heliconius erato petiverana, Heliconius erato erato, Heliconius erato cyrbia, Heliconius himera. Tissue: Wing-disk tissue collected from: 5th instar, early prepupal, late prepupal, 24 hours after pupation, precolor pupal stages (i.e. pupae > 48 hour after pupation, but before the development of wing pigmentation), 10 days after pupation

text: mixed, L5+

text: Heliconius erato/himera mixed EST library

text: mixed

text: mixed

characteristic: strain

text: wing disc

text: Heliconius erato pooled wing disc library

text: pBR322 Bluescript (SK+) system


text: National Eye Institute

characteristic: INSDC first public

text: live

text: Generic

text: RNA was extracted from pooled mouse lens. A directionally cloned cDNA library in the pGADT7-rec vector was constructed at Clontech (Laurel MD). EST analysis was performed at the NIH Intramural Sequencing Center (NISC). Analyzed data available through http://neibank.nei.nih.gov.

characteristic: description

text: adult

text: Eye

text: Mus musculus

text: Lens

text: Mouse lens. Y2H (nbf)

text: Plasmid