text: Kumamoto University

text: live

text: Generic

text: Embryonic stem cell (feeder free)

text: ES cells KMB6-6

text: country: Japan

text: Mus musculus

characteristic: organism

text: male

text: EGTC Gene Trap Library, KMB6-6, pU-21T


status: PUBLIC

characteristic: External Id

text: University of Pittsburgh School of Medicine

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: AAV8-ISceI.AO3 vector produced from recombinant adeno-associated virus (rAAV) vector plasmid, pAAV-ISceI.AO3 (accession number EU022316), was injected intraperitoneally into neonatal C57BL/6J male and female mice between 24 and 48 hours after birth. Five days post-injection, the livers were harvested. rAAV provirus genome libraries were constructed from genomic DNA extracted from a mixture of livers of 4 mice (gender not determined) and rAAV integration sites were identified as described previously (Inagaki et al., J. Virol. 81: 11290- 11303, 2007).

characteristic: description

text: neonate

tag: attribute

characteristic: dev-stage

text: liver

characteristic: organ

text: Mus musculus

text: male and/or female (not determined)

characteristic: sex

characteristic: strain

text: liver

characteristic: tissue-type

characteristic: title

text: recombinant adeno-associated virus

characteristic: vector


text: live

text: Generic

text: AAV8-ISceI.AO3 vector produced from recombinant adeno-associated virus (rAAV) vector plasmid, pAAV-ISceI.AO3 (accession number EU022316), was injected intraperitoneally into neonatal C57BL/6J male mice between 24 and 48 hours after birth. Six weeks post-injection, the livers were harvested. rAAV provirus genome libraries were constructed from liver genomic DNA and rAAV integration sites were identified as described previously (Inagaki et al., J. Virol. 81: 11290- 11303, 2007).

text: neonate

text: liver

text: Mus musculus

text: male

text: liver

text: recombinant adeno-associated virus


text: University of California-Davis; Institute for Crop Research in the Semi-Arid Tropics; National Research Centre on Plant Biotechnology; and J. Craig Venter Institute

text: live

text: Generic

text: Asha

characteristic: cultivar

text: Pigeonpea (Cajanus cajan) accession Asha was grown under greenhouse conditions to the seedling stage and transferred to continuous darkness for 2 days prior to use. Nuclei were isolated and embedded in low melting point agarose, restriction digested with BamHI and size selected by means of two rounds of pulsed field gel electrophoresis. Large size DNA fragments were ligated in vector pCC1BAC and transformed by electroporation in to Epicenter's E. coli EPI300-T1R cells. 34,560 clones from were obtained, with an average insert size of 115 kbp. BAC end sequences were obtained from ~25,000 clones.

text: Leaves

text: Cajanus cajan

text: BamHI

text: CccABb

text: Epicenter Copy Control pCC1BAC (BamHI partial digest clones)


text: live

text: Generic

text: Asha

text: Pigeonpea (Cajanus cajan) accession Asha was grown under greenhouse conditions to the seedling stage and transferred to continuous darkness for 2 days prior to use. Nuclei were isolated and embedded in low melting point agarose, restriction digested with Hind III and size selected by means of two rounds of pulsed field gel electrophoresis. Large size DNA fragments were ligated in vector pCC1BAC and transformed by electroporation in to Epicenter's E. coli EPI300-T1R cells. 34,560 clones from were obtained, with an average insert size of 120 kbp. BAC end sequences were obtained from ~25,000 clones.

text: Leaves

text: Cajanus cajan

characteristic: organism

text: HindIII

text: CccABa

text: Epicenter Copy Control pCC1BAC (HindIII partial digest clones)


characteristic: External Id

text: Bay Area Functional Genomics Consortium (BayGenomics)

text: live

text: Generic

text: Embryonic stem cell

characteristic: cell type

text: Mus musculus

text: Male

text: 129 ola

text: BayGenomics Gene Trap Library pGT0Lxf

text: plasmid


text: Texas A&M University

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: peripheral blood neutrophil

text: The CHORI-241 Horse (Equus caballus) BAC library has been constructed by Dr. Baoli Zhu in BACPAC Resources in collaboration with Dr. Doug Antczak in Equine Genetic Center, James A. Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University. High molecular weight DNA was prepared in embedded agarose plugs from thoroughbred male peripheral blood neutrophil cells by Dr. Helen Bird. The library has been constructed by using partially digested DNA with EcoRI plus EcoRI methylase. Size fractionated DNA was cloned into the pTARBAC2.1 vector between the EcoRI sites. The ligation products were transformed into DH10B eletrocompetent cells (BRL Life Technologies). The library represents approximately 11.8-fold total genomic representation. Average insert size is 171Kbp

text: adult

text: Equus caballus

text: male

text: thoroughbred

text: blood

text: CHORI-241 Equine BAC Library


text: Texas A&M University

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Horse BAC library

text: Equus caballus

text: HindIII

text: HindIII

text: Fibroblasts or leukocytes

text: INRA-LGBC


text: University of Minnesota

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: CH260 Female Turkey BAC library. Partially inbred commercial line, Nicholas Turkey Breeding Farms. For library and clone ordering information: http://bacpac.chori.org/turkey260.htm

characteristic: lab-host

text: Meleagris gallopavo

text: EcoRI

text: EcoRI

text: female

text: BAC


text: Texas A&M University

text: live

text: Generic

text: lymphocytes

text: adult

characteristic: dev-stage

text: Equus caballus

text: male

text: Thoroughbred

text: blood

text: Thoroughbred Genomic DNA


text: Texas A&M University

text: live

text: Generic

text: neutrophils

characteristic: description

text: adult

text: Equus caballus

text: male

characteristic: sex

text: Arabian

text: peripheral blood

text: Texas A&M University (TAMU) Equine BAC Library


text: University of Bristol

text: live

text: Generic

text: Assorted sequence characterized amplification regions from Orobanche minor.

text: Orobanche minor

text: Orobanche SCARs


text: USDA-ARS and University of Wisconsin

text: live

text: Generic

text: Cheonjudaego

text: Onion HindIII BAC library

text: Allium cepa

text: Onion BAC Library

text: BAC


text: USDA-ARS and University of Wisconsin

text: live

text: Generic

text: Doubled haploid 15197 (Seminis Seed Co)

text: DNA was isolated from onion (Allium cepa) doubled haploid 15197 (Seminis Seed Co) and purified by cesium chloride centrifugation DNA was sheared by nebulization, the 1-2 kb fraction was size-selected, end polished, ligated with BstXI adapters and cloned into the BstXI site of TIGR vector pHOS2 (a pBR322 derivative. Clones were propagated in E. coli DH5alpha to limit propagation of methylated DNA.

text: Etiolated seedlings

characteristic: organ

text: Allium cepa

text: BstXI

text: JBVS

text: pHOS2 (TIGR; a pBR322 derivative)


text: USDA-ARS and University of Wisconsin

text: live

text: Generic

text: Doubled haploid 15197 (Seminis Seed Co)

text: DNA was isolated from onion (Allium cepa) doubled haploid 15197 (Seminis Seed Co) and purified by cesium chloride centrifugation DNA was sheared by nebulization, the 1-2 kb fraction was size-selected, end polished, ligated with BstXI adapters and cloned into the BstXI site of TIGR vector pHOS2 (a pBR322 derivative. Clones were propagated in E. coli DH10B.

text: Etiolated seedlings

text: Allium cepa

text: BstXI

text: JBVR

characteristic: title

text: pHOS2 (TIGR; a pBR322 derivative)


text: University of Bristol

text: live

text: Generic

text: Assorted sequence characterized amplification regions from Orobanche picridis.

text: Orobanche picridis

text: Orobanche picridis SCARs


text: Technische Universitat Munchen, Center of Life and Food Sciences Weihenstephan, Plant Breeding

text: live

text: Generic

text: The BAC library was constructed in accordance with methods described previously (Choi and Wing 2000; Perteson et al. 2000). High molecular weight (HMW) DNA was extracted from young leaves of the sunflower genotype HA383 (PI 5788720). HMW genomic DNA was partially digested with HindIII and ligated to the vector pIndigoBac536 (Luo et al. 2001) derived from pBeloBAC11 (Kim et al. 1996). Ligations were transformed into E. coli competent cell DH10B. The BAC library supplied 8.0x haploid genome equivalents. The BAC library can be requested from the Clemson University Genomics Institute (http://www.genome.clemson.edu).

text: Young leaf

text: Helianthus annuus

text: Hind III

text: Hind III

text: Leaf

characteristic: tissue-type

text: BAC library from sunflower (Helianthus annuus) cultivar HA383

text: BAC

characteristic: vector


text: Niigata University

text: live

text: Generic

text: Arabidopsis thaliana T-DNA insertion lines Columbia background

text: Arabidopsis thaliana

characteristic: organism

text: Arabidopsis thaliana (Columibia) T-DNA-tag line of Kazusa DNA Research Institute


characteristic: External Id

text: Nanjing Agricultural University

text: live

text: Generic

text: suzhouqing

characteristic: cultivar

text: country: China

text: Brassica rapa subsp. chinensis

text: Brassica rapa subsp. chinensis cv. suzhouqing


text: University of Parma

characteristic: INSDC center name

text: live

text: Generic

text: Leaf blade

text: Cistus incanus

text: hermaphrodite

text: Cistus incanus RAPD fragment

text: pGem T-easy (Promega)


text: University of Parma

characteristic: INSDC first public

text: live

text: Generic

text: Leaf blade

text: Rhus coriaria

text: hermaphrodite

text: Rhus coriaria RAPD fragment

text: pGem T-easy (Promega)


text: University of Parma

characteristic: INSDC last update

text: live

text: Generic

text: Leaf blade

text: Rubus caesius

text: hermaphrodite

text: Rubus caesius RAPD fragment

text: pGem T-easy (Promega)


text: State University of Campinas

text: live

characteristic: INSDC status

text: Generic

text: somatic

characteristic: cell type

text: GSS Library was prepared by shearing total DNA of Moniliophthora perniciosa in a nebulizer (Invitrogen) to a size range of 1 to 5 kb, following the manufacturer's protocol. DNA fragments were size-fractionated by agarose gel electrophoresis and two size ranges were independently cloned into the PCR4-Topo vector (Invitrogen): 0.5 to 2 Kb and 2 to 4 Kb. Ligation products were transformed into electrocompetent E. coli (DH10B) by electroporation and individual white colonies were picked for plasmid purification and sequencing.

text: saprotrophic

text: Theobroma cacao L.

text: Moniliophthora perniciosa

characteristic: strain

text: dikaryotic mycelium

text: Plasmid

text: PCR4-blunt Topo (Invitrogen)


text: University of California-Davis; Institute for Crop Research in the Semi-Arid Tropics; National Research Centre on Plant Biotechnology; and J. Craig Venter Institute

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Asha

text: Pigeonpea (Cajanus cajan) accession Asha was grown under greenhouse conditions to the seedling stage and transferred to continuous darkness for 2 days prior to use. Nuclei were isolated and embedded in low melting point agarose, restriction digested with Hind III and size selected by means of two rounds of pulsed field gel electrophoresis. Large size DNA fragments were ligated in vector pCC1BAC and transformed by electroporation in to Epicenter's E. coli EPI300-T1R cells. 34,560 clones from were obtained, with an average insert size of 120 kbp. BAC end sequences were obtained from ~25,000 clones.

text: Leaves

text: Cajanus cajan

text: HindIII/BamHI

text: Epicenter Copy Control pCC1BAC


text: National Institute of Plant Genome Research

text: live

text: Generic

text: Xanthi

text: seedling

characteristic: dev-stage

text: Nicotiana tabacum

text: Genome walking library of Nicotiana tabacum


text: UNICAMP

text: live

text: Generic

text: Zea mays

text: Zea mays BAC

text: BAC


text: Institute for Animal Health

text: live

text: Generic

text: Suppression subtractive hybridization library between S. Dublin SD3246 and S. Gallinarum SG9

characteristic: description

characteristic: isolate

text: Salmonella enterica subsp. enterica serovar Dublin

text: Salmonella Dublin subtractive hybridization library


text: University of Alberta

text: live

text: Generic

text: Homo sapiens

text: liver

text: Homo sapiens liver representational difference analysis product

characteristic: title


text: Cold Spring Harbor Laboratory

text: live

text: Generic

text: Landsberg

text: Lines of Arabidopsis thaliana were generated which each contain a Ds transposon carrying a glucuronidase reporter gene. Genomic DNA flanking the transposon insertion in each line was amplified by PCR and directly sequenced. More information is available at http://genetrap.cshl.edu.

text: Arabidopsis thaliana

text: Arabidopsis thaliana Landsberg Ds insertion lines


text: J. Craig Venter Institute

text: live

text: Generic

text: location: Ecuador: 134 miles NE of Galapagos, open ocean; latitude: 1.2641666666666667; longitude: -90.295; depth: 2386 meters; salinity: 32.6; temperature: 27.8; sampling site monthly chlorophyll level: >0.22 mg/kL; sampling site yearly chlorophyll level: >0.28 +/- 0.02 mg/kL; lo_filter_size: 0.1 microns; hi_filter_size: 0.8 microns; collection_date: Feb 01 2004 04:16PM

text: marine metagenome

text: Global-Ocean-Sampling_GS-26-01-01-1P3-1P6KB


text: J. Craig Venter Institute

text: live

text: Generic

text: marine metagenome

text: Global-Ocean-Sampling_GS-26-02-01-1P0-1P3KB


text: J. Craig Venter Institute

text: live

text: Generic

text: location: Ecuador:DevilÃs Crown, Floreana Island, Coastal Environment; latitude: -1.2161111111111111; longitude: -90.42277777777778; depth: 2.3 meters; salinity: 34.9; temperature: 25.5; sampling site monthly chlorophyll level: >0.40 mg/kL; sampling site yearly chlorophyll level: >0.38 +/- 0.03 mg/kL; lo_filter_size: 0.1 microns; hi_filter_size: >0.8 microns; collection_date: Feb 04 2004 11:41AM

text: marine metagenome

text: Global-Ocean-Sampling_GS-27-01-01-1P8-2P0KB


text: J. Craig Venter Institute

text: live

text: Generic

text: location: Ecuador:Coastal Floreana; latitude: -1.2169444444444444; longitude: -90.31972222222223; depth: 156 meters; sampling site monthly chlorophyll level: >0.35 mg/kL; sampling site yearly chlorophyll level: >0.35 +/- 0.02 mg/kL; lo_filter_size: 0.1 microns; hi_filter_size: 0.8 microns; collection_date: Feb 04 2004 03:47PM

text: marine metagenome

text: Global-Ocean-Sampling_GS-28-01-01-1P6-2P0KB


text: J. Craig Venter Institute

text: live

text: Generic

text: location: Ecuador: North James Bay, Santigo Island, Coastal environment; latitude: -0.2; longitude: -90.83527777777778; depth: 12 meters; salinity: 34.5; temperature: 26.2; sampling site monthly chlorophyll level: >0.40 mg/kL; sampling site yearly chlorophyll level: >0.39 +/- 0.09 mg/kL; lo_filter_size: 0.1 microns; hi_filter_size: 0.8 microns; collection_date: Feb 08 2004 06:03PM

text: marine metagenome

text: Global-Ocean-Sampling_GS-29-01-01-1P0-1P3KB


text: J. Craig Venter Institute

text: live

text: Generic

text: location: Ecuador: Roca Redonda, Warm Seep; latitude: 0.2722222222222222; longitude: -91.63333333333334; depth: 19 meters; temperature: 26.9; lo_filter_size: 0.1 microns; hi_filter_size: 0.8 microns; collection_date: Feb 09 2004 11:42AM

text: marine metagenome

text: Global-Ocean-Sampling_GS-30-01-01-1P3-1P6KB


text: J. Craig Venter Institute

text: live

text: Generic

text: marine metagenome

text: Global-Ocean-Sampling_GS-30-02-01-1P0-1P3KB


text: J. Craig Venter Institute

text: live

text: Generic

text: location: Ecuador: Fernandina Island, Coastal Upwelling; latitude: -0.3011111111111111; longitude: -91.65166666666667; depth: 19.6 meters; temperature: 18.6; sampling site monthly chlorophyll level: >0.35 mg/kL; sampling site yearly chlorophyll level: >0.39 +/- 0.03 mg/kL; lo_filter_size: 0.1 microns; hi_filter_size: 0.8 microns; collection_date: Feb 10 2004 02:43PM

text: marine metagenome

text: Global-Ocean-Sampling_GS-31-01-01-1P3-1P8KB


text: J. Craig Venter Institute

text: live

text: Generic

text: location: Ecuador:Mangrove on Isabella Island; latitude: -0.5938888888888889; longitude: -91.06944444444444; depth: 1.6 meters; temperature: 25.4; lo_filter_size: 0.1 microns; hi_filter_size: 0.8 microns; collection_date: Feb 11 2004 11:30AM

text: marine metagenome

text: Global-Ocean-Sampling_GS-32-01-01-1P3-1P6KB


text: J. Craig Venter Institute

text: live

text: Generic

text: marine metagenome

text: Global-Ocean-Sampling_GS-32-02-01-1P0-1P3KB


text: East China University of Science and Technology

text: live

text: Generic

text: Suppression subtractive hybridization library constructed with a PCR-Select Bacterial Genome Subtraction Kit (Clontech, Palo Alto, CA, USA). Genomic DNA from Edwardsiella tarda EIB202 was used as tester and genomic DNA from Edwardsiella tarda ATCC-15947 as driver.

text: Edwardsiella tarda

text: Edwardsiella tarda EIB202 subtractive hybridization library

text: Plasmid

text: pGEM-T


text: University of Bristol

text: live

text: Generic

text: sequence generated from sequence characterised amplified region (SCAR) markers

text: Orobanche minor

text: floral bud

characteristic: tissue-type

text: O. minor SCARs


text: University of Bristol

text: live

text: Generic

text: Orobanche litorea

text: floral bud

text: O. littorea SCARs


text: University of Bristol

text: live

text: Generic

text: Orobanche caryophyllacea

text: floral bud

text: O. caryophyllaceae SCARs


text: The Institute of Physical and Chemical Research (RIKEN)

text: live

text: Generic

text: mating_type: h plus

text: Schizosaccharomyces pombe

text: F-pombe


text: live

text: Generic

text: Terfezia boudieri

text: Terfezia boudieri AFLP fragment


text: University of Delaware

text: live

text: Generic

text: Virioplankton within diffuse flow water emanating from the sides of deep-sea hydrothermal vent chimneys were collected and concentrated using tangential-flow filtration according to Wommack et al. 2004. Deep Sea Res. 51:1781. Randomly amplified polymorphic DNA-PCR (RAPD-PCR) was used to generate sequence amplicons from virioplankton DNA. RAPD-PCR products were directly cloned into a Topo-TA vector and sequenced.

text: uncultured deep-sea hydrothermal vent virus

text: Plasmid

text: pCR(II-TOPO vector (Invitrogen, Carlsbad, CA)

characteristic: vector


text: University of Delaware

text: live

text: Generic

text: Virioplankton within diffuse flow water emanating from the sides of deep-sea hydrothermal vent chimneys were collected and concentrated using tangential-flow filtration according to Wommack et al. 2004. Deep Sea Res. 51:1781. A linker amplified shotgun library (LASL) was constructed from these viral concentrates. Briefly, viral DNA was randomly sheared to ~2-3 kb fragments and short linker priming sites were ligated to the ends of these fragments. PCR using primers complementary to the linkers was performed for an unbiased amplification of the viral genomic DNA fragments. These PCR-amplified fragments were ligated into the transcription-free vector pSMART (Lucigen, Corp) to construct the sequencing library. LASL construction was done using the Nanoclone service of Lucigen Corporation (Middleton, WI).

characteristic: organism

text: Plasmid

text: pSMART (Lucigen Corp., Middletown, WI)


characteristic: External Id

text: J. Craig Venter Institute

text: live

text: Generic

text: location: Ecuador: Punta Cormorant, Floreana Island, Hypersaline Lagoon; latitude -1.2283333333333333; longitude: -90.42916666666666; depth: 0.3 meters; salinity: 63.4; temperature: 37.6; lo_filter_size: 0.1 microns; hi_filter_size: 0.8 microns; collection_date: Feb 19 2004 01:35PM

text: marine metagenome

text: Global-Ocean-Sampling_GS-33-01-01-1P3-1P8KB


text: J. Craig Venter Institute

text: live

text: Generic

text: location: Ecuador: North Seamore Island, Coastal environment; latitude: -0.38305555555555554; longitude: -90.27972222222222; depth: 35 meters; temperature: 27.5; sampling site monthly chlorophyll level: >0.36 mg/kL; sampling site yearly chlorophyll level: >0.35 +/- 0.02 mg/kL; lo_filter_size: 0.1 microns; hi_filter_size: 0.8 microns; collection_date: Feb 19 2004 05:06PM

text: marine metagenome

text: Global-Ocean-Sampling_GS-34-01-01-1P5-1P8KB


text: J. Craig Venter Institute

text: live

text: Generic

text: location: Ecuador: Wolf Island, Coastal environment; latitude: 1.3891666666666667; longitude: -91.81694444444445; depth: 71 meters; salinity: 34.5; temperature: 21.8; sampling site monthly chlorophyll level: >0.28 mg/kL; sampling site yearly chlorophyll level: >0.31 +/- 0.02 mg/kL; lo_filter_size: 0.1 microns; hi_filter_size: 0.8 microns; collection_date: Mar 01 2004 04:44PM

text: marine metagenome


text: J. Craig Venter Institute

text: live

text: Generic

text: location: Ecuador: Cabo Marshall, Isabella Island, Coastal environment; latitude: -0.020833333333333332; longitude: -91.19777777777777; depth: 67 meters; salinity: 34.6; temperature: 25.8; sampling site monthly chlorophyll level: >0.65 mg/kL; sampling site yearly chlorophyll level: >0.45 +/- 0.05 mg/kL; lo_filter_size: 0.1 microns; hi_filter_size: 0.8 microns; collection_date: Mar 02 2004 12:52PM

text: marine metagenome


text: Lawrence Berkeley National Laboratory

text: live

text: Generic

text: The P{wHy} deletion-generator element and mutagenesis protocol are described in Huet et al., 2002, Proc. Natl. Acad. Sci. USA, 99:9948--9953; the complete sequence of the P{wHy} element is available (GenBank accession number AF516513). Initial flanking sequence data were determined using the Universal Fast Walking (UFW) procedure, as described by Myrick and Gelbart, 2002, Gene 284:125-131. Final sequence data were determined using inverse PCR, as part of the Drosophila Gene Disruption Project (Bellen H.J. et al. 2004, Genetics 167:761-781).

text: Drosophila melanogaster

text: P{wHy} element transposon insertion site flanking sequences


text: Institute of Experimental Botany

text: live

text: Generic

text: Imperial

characteristic: cultivar

text: library was prepared from chromosome arm 1RS flow sorted from ditelocentric 1RS wheat/rye (Chinese Spring /Imperial) addition line (21 + 1RS)

text: Escherichia coli DH10B

characteristic: lab-host

text: Secale cereale

text: HindIII

text: HindIII

text: BAC


text: Institute of Experimental Botany

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: Imperial

text: Escherichia coli DH10B

text: Secale cereale

text: BamHI

text: BamHI

text: BAC


text: Bielefeld University, CeBiTec

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: KWS2320 (double haploid, monogerm breeding line)

text: Randomly sheared genomic DNA of the sugar beet DH line KWS2320 was cloned into pCC1FOS. Clones were arrayed into 384well plates, and fosmids were end-sequenced using an ABI3730XL from both ends using pCC1 fwd (5-GGATGTGCTGCAAGGCGATTAAGTTGG-3) and rev (5-CTCGTATGTTGTGTGGAATTGTGAGC-3) sequencing primers. The fosmid ends have been produced by Prisca Viehoever at CeBiTec, Bielefeld University.

text: Beta vulgaris subsp. vulgaris

text: blunt

text: blunt

text: IGS-SCF-1033 Bvu-KWS2320 fosmid genomic library


text: Max-Planck-Institut fuer Zuechtungsforschung

text: live

text: Generic

text: Genomic DNA from a homozygous transgenic Arabidopsis thaliana plant (PM) transformed with the T-DNA from vector pGPTV expressing GUS gene under the control of AtWRKY40 promoter was digested with HindIII and inverse PCR was carried out with primers 489 (GCGTGGACCGCTTGCTGCAACT) and 550 (CCGCAATTATACATTTAATACGCG) after self ligation. The 1.2 kb PCR amplification product was gel isolated and sequenced using primer 550.

text: Arabidopsis thaliana

text: Columbia 0

characteristic: strain

text: Arabidopsis thaliana T-DNA insertion lines BU-PM-1


text: Cnr, Ist. di Biologia e Biotecnologia Agraria

text: live

text: Generic

characteristic: NCBI submission model

text: specimen_voucher: G12949

text: Phaseolus vulgaris

text: leaf

text: Pv-G12949 HindIII BAC library

text: pIndigoBAC-5 HindIII


text: Instituto de Biotecnologia INTA-Castelar

text: live

text: Generic

characteristic: NCBI submission package

text: Solanum pennellii

text: Solanum pennellii BAC library

text: Bac

text: pBeloBacII


text: live

text: Generic

text: Solanum pennellii

text: Solanum pennellii COS library

text: Cosmid

text: pCLD 04541


text: Indian Agricultural Research Institute

text: live

text: Generic

text: Four weeks old

characteristic: dev-stage

text: Brassica juncea

text: Leaf (LF)

text: Brassica juncea BEC-144 Resistant Gene analogs

text: Plasmid

text: pGEMTEasy


text: National Institute for Basic Biology

text: live

text: Generic

text: substrain = MG1655

characteristic: description

text: Escherichia coli str. K-12 substr. MG1655

text: Escherichia coli K12 substrain MG1655


text: National Institute of Agrobiological Sciences

text: live

text: Generic

text: 5th instar larvae day3

text: Bombyx mori

text: mixed

characteristic: sex

text: posterior silkgland

text: Bombyx mori posterior silkgland p50T 5th instar larvae day3


text: University of Toronto

text: live

text: Generic

text: Embryonic Stem Cell

characteristic: cell type

characteristic: cell-line

text: Mus musculus

text: Male

characteristic: title

text: Gene trap insertion mutagen

text: UPA


text: University of Toronto

text: live

text: Generic

text: Embryonic Stem Cell

text: Mus musculus

characteristic: organism

text: Male

text: Gene trap insertion mutagen

characteristic: vector


characteristic: External Id

text: University of California, Davis, Catholic University of Brasilia, and The J. Craig Venter Institute

text: live

text: Generic

text: Accession: V14167. Details of the A. duranensis BAC library are published and available through PubMed: Guimaraes et al. (2008) BAC libraries construction from the ancestral diploid genomes of the allotetraploid cultivated peanut. BMC Plant Biology 8:14 doi:10.1186/1471-2229-8-14.

text: E. coli, ElectroMAX DH10B, Invitrogen

text: Leaves

characteristic: organ

text: Arachis duranensis

text: HindIII


text: Michigan State University

characteristic: INSDC center name

text: live

text: Generic

text: Minghui 63

characteristic: cultivar

text: Genomic DNA was isolated from leaves. Select regions were amplified by PCR and sequenced by dideoxy-based sequencing.

text: Oryza sativa Indica Group

text: leaves

characteristic: tissue-type

text: O. sativa Minghui 63 seq from PCR product


text: Michigan State University

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Zhen Shan 97

text: Oryza sativa Indica Group

text: leaves

text: O. sativa Zhen Shan 97 seq from PCR product


text: Michigan State University

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Nippon Bare

text: Oryza sativa Japonica Group

text: leaves

text: O. sativa Nippon Bare seq from PCR product


text: Michigan State University

text: live

text: Generic

text: LTH

characteristic: description

text: Oryza sativa Japonica Group

text: leaves

text: O. sativa LTH seq from PCR product


text: Michigan State University

text: live

text: Generic

text: M 202

text: Oryza sativa Japonica Group

text: leaves

text: O. sativa M 202 seq from PCR product


text: Michigan State University

text: live

text: Generic

text: Tainung 67

text: Oryza sativa Japonica Group

text: leaves

text: O. sativa Tainung 67 seq from PCR product


text: Michigan State University

text: live

text: Generic

text: Moroberekan

text: Oryza sativa Japonica Group

text: leaves

text: O. sativa Moroberekan seq from PCR product


text: Michigan State University

text: live

text: Generic

text: Rayada

text: Oryza sativa Indica Group

text: leaves

text: O. sativa Rayada seq from PCR product


text: Michigan State University

text: live

text: Generic

text: Dom Sufid

text: Oryza sativa Japonica Group

text: leaves

text: O. sativa Dom Sufid seq from PCR product


text: Michigan State University

text: live

text: Generic

text: Azucena

text: Oryza sativa Japonica Group

text: leaves

text: O. sativa Azucena seq from PCR product


text: Michigan State University

text: live

text: Generic

text: Cypress

text: Oryza sativa Japonica Group

text: leaves

text: O. sativa Cypress seq from PCR product


text: Michigan State University

text: live

text: Generic

text: Dular

text: Oryza sativa Indica Group

text: leaves

text: O. sativa Dular seq from PCR product


text: Michigan State University

text: live

text: Generic

text: FR 13 A

text: Oryza sativa Indica Group

text: leaves

text: O. sativa FR 13 A seq from PCR product


text: Michigan State University

text: live

text: Generic

text: N 22

text: Oryza sativa Indica Group

text: leaves

text: O. sativa N 22 seq from PCR product


text: Michigan State University

text: live

text: Generic

text: Aswina

text: Oryza sativa Indica Group

text: leaves

text: O. sativa Aswina seq from PCR product


text: Michigan State University

text: live

text: Generic

text: IR 64

text: Oryza sativa Indica Group

text: leaves

text: O. sativa IR 64 seq from PCR product


text: Michigan State University

text: live

text: Generic

text: Pokkali

text: Oryza sativa Indica Group

text: leaves

text: O. sativa Pokkali seq from PCR product


text: Michigan State University

text: live

text: Generic

text: Sadu Cho

text: Oryza sativa Indica Group

text: leaves

text: O. sativa Sadu Cho seq from PCR product


text: Michigan State University

text: live

text: Generic

text: Oryza sativa Indica Group

text: leaves

text: O. sativa SHZ2 seq from PCR product


text: Michigan State University

text: live

text: Generic

text: Swarna

text: Oryza sativa Indica Group

text: leaves

text: O. sativa Swarna seq from PCR product


text: National Institute of Agrobiological Resources

text: live

text: Generic

text: Hitomebore

text: Oryza sativa Japonica Group

characteristic: strain

text: strain NC2773 PCR product directly amplified from rice genomic DNA


text: live

text: Generic

text: Hitomebore

text: Oryza sativa Japonica Group

text: strain NC2776 PCR product directly amplified from rice genomic DNA


text: live

text: Generic

text: Hitomebore

text: Oryza sativa Japonica Group

text: strain NC2788 PCR product directly amplified from rice genomic DNA


text: live

text: Generic

text: Hitomebore

text: Oryza sativa Japonica Group

text: strain NC2789 PCR product directly amplified from rice genomic DNA


text: live

text: Generic

text: Hitomebore

text: Oryza sativa Japonica Group

text: strain NC2775 PCR product directly amplified from rice genomic DNA


text: live

text: Generic

text: Hitomebore

text: Oryza sativa Japonica Group

text: strain NC2792 PCR product directly amplified from rice genomic DNA


text: live

text: Generic

text: Hitomebore

text: Oryza sativa Japonica Group

text: strain NC2777 PCR product directly amplified from rice genomic DNA


text: live

text: Generic

text: Hitomebore

text: Oryza sativa Japonica Group

text: strain NC2774 PCR product directly amplified from rice genomic DNA


text: live

text: Generic

text: Hitomebore

text: Oryza sativa Japonica Group

text: strain NC2778 PCR product directly amplified from rice genomic DNA


text: live

text: Generic

text: Hitomebore

text: Oryza sativa Japonica Group

text: strain NC2779 PCR product directly amplified from rice genomic DNA


text: live

text: Generic

text: Hitomebore

text: Oryza sativa Japonica Group

text: strain NC2782 PCR product directly amplified from rice genomic DNA


text: live

text: Generic

text: Hitomebore

text: Oryza sativa Japonica Group

text: strain NC2783 PCR product directly amplified from rice genomic DNA


text: live

text: Generic

text: Hitomebore

text: Oryza sativa Japonica Group

text: strain NC2793 PCR product directly amplified from rice genomic DNA


text: live

text: Generic

text: Hitomebore

text: Oryza sativa Japonica Group

text: strain NC2781 PCR product directly amplified from rice genomic DNA


text: Max-Planck-Institut fuer Zuechtungsforschung

text: live

text: Generic

text: PCR was performed on DNA from Arabidopsis thaliana plants (T1) which were transformed with the T-DNA from vector pAC106. The lines contain one or more T-DNA insertions. The DNA fragment(s) resulting from the PCR were directly sequenced to determine the genomic sequence flanking the insertion. Sequences displaying significant similarity to the A. thalaina nuclear genome sequence were processed for submission. T-DNA derived sequences were removed

text: Arabidopsis thaliana

text: Columbia 0

text: Arabidopsis thaliana T-DNA insertion lines


text: The University of Arizona

text: live

text: Generic

text: These BES have been generated for validation of BAC clones for The Maize Genome Sequencing Project. BES generated are from different libraries (ZMMBBb and ZMMBBc). Library details are as follows: ZMMBBb Library: Vector - pBeloBAC11; HindIII site Individual clones and the entire library are distributed by CUGI at http://www.genome.clemson.edu/orders/ ZMMBBc Library: Vector - pTARBAC2.1(for plates 1-288; EcoRI site), pTARBAC1.3(for plates 289-576; MboI site) Individual clones and the entire library are distributed by Pieter de Jong at http://www.chori.org/bacpac/

characteristic: lab-host

text: Zea mays

text: Plasmid


text: Purdue University

text: live

text: Generic

text: DNA sequence obtained from PCR products amplified from B73 genomic DNA using gene target - specific primers. The PCR product was sequenced from both the 5' and 3' ends.

text: Zea mays

text: Maize TILLING Project Target Sequences B73


text: Lawrence Berkeley National Laboratory

text: live

text: Generic

text: These are the DNA sequences flanking the insertion sites of a collection of Minos transposon insertion mutants of Drosophila melanogaster that were produced by the Drosophila Gene Disruption Project in the laboratory of Hugo Bellen. The transposon mutator is the synthetic construct MiET1 (Metaxakis et al. (2005) Genetics 171: 571-581; FlyBase symbol: Mi{ET1}). Sequences flanking the insertion sites of mutants were amplified by inverse PCR and sequenced at the Lawrence Berkeley National Laboratory using an unpublished modification of the protocol of Bellen et al. 2004 (Genetics 167: 761-781; FlyBase ID:FBrf0179132). Protocols may be downloaded from the project Web site at: http://flypush.imgen.bcm.tmc.edu/pscreen/.

text: Drosophila melanogaster

text: MB transposon insertion flanking sequences


text: National Institute of Genetics

text: live

text: Generic

text: Hemicentrotus pulcherrimus

text: SacI

text: Hemicentrotus pulcherrimus whole genome BAC library, BUB1


text: National Institute of Genetics

text: live

text: Generic

text: Notamacropus eugenii

text: Sacl

text: male

characteristic: sex

text: Macropus eugenii whole genome BAC library, MEB1


text: National Institute of Genetics

text: live

text: Generic

text: HpaI site

text: Notamacropus eugenii

text: female

text: Macropus eugenii,Chromosome X specific library,MEFX

characteristic: title


text: University of Cambridge

text: live

text: Generic

text: BAC genomic library generated using a partial enzymatic digestion with MboI. Vector cloning site is BamHI. Average insert size is 123 kb. Library contains 48 X 384 well plates and is available from Amplicon Express, Pullman, WA, USA.

text: Heliconius melpomene

characteristic: organism

text: Leiden

text: Larval

text: Heliconius melpomene genomic BAC library

text: BAC


text: University of Cambridge

text: live

text: Generic

text: Heliconius erato etylus was crossed with Heliconius himera. Unrelated F1 progeny were mated and AFLP templates were prepared using F2 progeny and parents. AFLP markers were separated using polyacrylamide gel electrophoresis then specific bands excised and resuspended in water. AFLP bands were reamplified and sequenced.

text: Heliconius erato etylus x Heliconius himera

text: Adult

text: Heliconius erato genomic AFLP DNA


text: University of Cambridge

text: live

text: Generic

text: AFLP templates were prepared using H. melpomene brood 44 F2 progeny, F1 parents and grandparents using half reactions of AFLP analysis system II (Invitrogen life technologies) according to manufacturers instructions, with EcoR1 and Mse1 restriction enzymes. AFLP markers were separated using polyacrylamide gel electrophoresis (Acrylamide 6%, 1xTBE, 7 M Urea) at 1750V, 80W for 1.5 to 3 hours. Acrylamide gels were stained with Silver Sequence (Promega) to visualize DNA bands, which were then excised and resuspended in water. AFLP bands were reamplified and sequenced using BigDye terminator v3.1 (Applied Biosystems) using an ABI3730.

text: Heliconius melpomene

text: Brood 44

text: Adult

text: Heliconius melpomene genomic AFLP DNA


text: Max Planck Institute for Developmental Biology

text: live

text: Generic

text: PCR products where obtained with specific primers from genomic DNA and sanger-sequenced using the same primers

text: Arabidopsis thaliana

text: Bur-0, CS22679

text: Bur-0, validation PCR products


text: Institution of crop science, Chinese Academy of Agricultural Sciences (CAAS)

text: live

text: Generic

text: Thinopyrum intermedium

text: Th. intermedium chromosome 2Ai-2 DNA inserts library


text: University of Virginia

text: live

text: Generic

text: PCR product

text: Danio rerio

text: mixed

text: TL

text: Zebrafish TL custom amplicons


text: National Institute of Genetics

text: live

text: Generic

text: Monosiga ovata

text: Sacl

text: Monosiga ovata whole genome BAC library, MOBS

characteristic: vector


characteristic: External Id

text: National Institute of Genetics

text: live

text: Generic

text: Monosiga ovata

text: HpaI

text: Monosiga ovata whole genome Fosmid library, MOF


text: CNRS

text: live

text: Generic

text: Medicago truncatula

text: MERE1 Transposon insertion site

text: Plasmid

text: pGEM-Teasy


text: Stanford University and the Howard Hughes Medical Institute

text: live

text: Generic

text: Vector sequences have been trimmed from submission.

text: Drosophila melanogaster

text: piggyBac Transposon Insertion Sites

text: Transposon

text: pXL-BacII-SAstopDsRed (Genbank # EU257621)


text: RIKEN Genomic Sciences Center

text: live

text: Generic

text: leucocyte

characteristic: cell type

text: Macaca fuscata fuscata

text: SacI

text: SacI

text: male

text: MSB2 Macaca Male BAC Library


text: Iwate Biotechnology Research Center

characteristic: INSDC center name

text: live

text: Generic

text: PI 159236

text: Capsicum chinense

text: Capsicum chinense PI 159236 AFLP marker


text: Iwate Biotechnology Research Center

text: live

text: Generic

text: PI 159236

characteristic: cultivar

text: Capsicum chinense

text: pBeloBAC Capsicum chinense genome DNA


text: Iwate Biotechnology Research Center

text: live

text: Generic

text: PI 159236

text: Capsicum chinense

text: Capsicum chinense PI 159236 RGA marker


text: Iwate Biotechnology Research Center

text: live

text: Generic

text: LS 1839-2-4

text: Capsicum frutescens

text: Capsicum frutescens LS 1839-2-4 genomic DNA


text: Iwate Biotechnology Research Center

text: live

text: Generic

text: KOS

text: Capsicum annuum

text: Capsicum annuum KOS genomic DNA


text: Iwate Biotechnology Research Center

text: live

text: Generic

text: NDN

text: Capsicum annuum

text: Capsicum annuum NDN genomic DNA


text: UMR-CNRS-8618, INRA, Universite Paris-sud

text: live

text: Generic

text: Kami et al (2006) Theor Appl Genet 112: 987-998.

text: E. coli DH10B

text: young leaves

characteristic: organ

text: Phaseolus vulgaris

text: HindIII

text: hermaphrodite

text: Phaseolus vulgaris BAT93 genomic DNA BAC library

text: pIndigoBAC


text: CMBI, NCMLS, Radboud University Nijmegen

characteristic: INSDC first public

text: live

text: Generic

text: country: Netherlands. isolation_source: Blaberus sp. var. Amsterdam.

text: Nyctotherus ovalis

text: Nyctotherus ovalis partial chromosome


characteristic: INSDC last update

text: live

text: Generic

text: Nyctotherus ovalis

text: Nyctotherus ovalis complete chromosome


text: United States Department of Agriculture (USDA), Agricultural Research Service (ARS)

text: live

characteristic: INSDC status

text: Generic

text: A partial genomic library constructed from inverse PCR products that contain O. nubialis genome regions that flank multiple microsatellite associated interspersed nuclear element 1, OnMINE-1, Helitron-like transposable elements.

text: adult

characteristic: dev-stage

text: Ostrinia nubilalis

text: male and female

text: bivoltine Z-pheromone strain

text: thoracic

characteristic: tissue-type

text: ARS-CICGRU OnMINE-1i

text: plasmid

text: pGEM-T


text: Yangzhou University

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: fragments generated from the suppression subtractive hybridization of S. pullorum C79-13 from S. gallinarum Sg9 or S enteritidis CMCC(B) 50041 using the PCR-Select bacterial genome subtraction kit(Clontech, Takara). The fragments were cloned into plasmid PCR2.1 vector using the Advantage PCR cloning kit(Clontech, Takara). S. pullorum C79-13 is a virulent strain for poultry.

characteristic: description

text: Salmonella enterica subsp. enterica serovar Pullorum

text: EcoRI

text: EcoRI

text: C79-13 (CVCC 533)

text: subtracted fragments from Salmonella pullorum C79-13


text: National Institute of Agrobiological Sciences

text: live

text: Generic

text: Bos taurus

text: Bos taurus Y chromosome specific RAPD marker


text: Korea Research Institute Bioscience & Biotechnology

text: live

text: Generic

text: Homo sapiens

text: Homo sapiens DNA (Kim Y)


text: live

text: Generic

text: Pan troglodytes

text: Pan troglodytes DNA (Kim Y)


text: live

text: Generic

text: Pongo sp.

text: Pongo sp. DNA (Kim Y)


text: live

text: Generic

text: Macaca mulatta

text: Macaca mulatta DNA (Kim Y)


text: live

text: Generic

text: Macaca fascicularis

text: Macaca fascicularis DNA (Kim Y)


text: live

text: Generic

text: Mandrillus sphinx

text: Mandrillus sphinx DNA (Kim Y)


text: live

text: Generic

text: Chlorocebus aethiops

text: Chlorocebus aethiops DNA (Kim Y)


text: live

text: Generic

text: Piliocolobus badius

text: Procolobus badius DNA (Kim Y)


text: live

text: Generic

text: Trachypithecus sp.

text: Trachypithecus sp. DNA (Kim Y)


text: live

text: Generic

text: Ateles geoffroyi

text: Ateles geoffroyi DNA (Kim Y)


text: live

text: Generic

text: Saguinus midas

text: Saguinus midas DNA (Kim Y)


text: live

text: Generic

text: Aotus nigriceps

text: Aotus nigriceps DNA (Kim Y)


text: live

text: Generic

text: Callithrix jacchus

text: Callithrix jacchus DNA (Kim Y)


text: live

text: Generic

text: Lemur catta

text: Lemur catta DNA (Kim Y)


text: Wellcome Trust Sanger Institute

text: live

text: Generic

text: Plasmodium falciparum 3D7

characteristic: strain

text: Plasmodium falciparum 3D7 shotgun sequence reads


text: University of Pennsylvania School of Medicine

text: live

text: Generic

text: Jurkat

text: Amplified PCR products were directly sequenced using pyrosequencing as implemented by 454 Life Sciences. An HIV-based vector transducing GFP and pseudotyped with VSV-G envelope was used.

text: Homo sapiens

text: AvrII

text: NheI

characteristic: title


text: live

text: Generic

text: Jurkat

text: Homo sapiens

characteristic: organism

text: MseI


text: Iowa State University

text: live

text: Generic

text: Sequences flanking Mu transposon insertion sites were amplified by MuTA PCR and topo-cloned.

characteristic: isolate

text: Zea mays

text: maize MuTA library emp*02S-0288 B2B


characteristic: External Id

text: Iowa State University

text: live

text: Generic

text: Zea mays

text: maize MuTA library dek*0285 C11C


text: Iowa State University

text: live

text: Generic

text: MJ 03-8097-2/8103-2

text: Zea mays

text: maize MuTA library o5-PS3038 C12D


text: Iowa State University

text: live

text: Generic

text: Zea mays

text: maize MuTA library emp*02S-0254 C6D


text: Iowa State University

text: live

text: Generic

text: Zea mays

text: maize MuTA library emp*02S-0089 C8C


text: Iowa State University

text: live

text: Generic

text: Zea mays

text: maize MuTA library emp*02S-0390 M10B


text: Research Institute for the Biology of Farm Animals (FBN)

text: live

text: Generic

text: lymphocyte

characteristic: cell type

text: CLONE_LIBRARY used: see Zhu et al. Mamm Genome 10:706-709, 1999

text: Bos taurus

text: male

characteristic: sex

text: Jersey

text: Bos taurus BAC library

text: BAC

characteristic: vector


text: Laboratory of Ornithology, Cornell University

characteristic: INSDC center name

text: live

text: Generic

text: Thryophilus pleurostictus

text: Banded wren genomic library


text: Biology Centre ASCR, Institute of Entomology

characteristic: INSDC first public

text: live

text: Generic

text: 5th instar larva

text: Cydia pomonella

text: female

text: Malpighian tubules

text: Cydia pomonella W-chromosome library

text: Plasmid


text: SGGW Warsaw Agricultural University

characteristic: INSDC last update

text: live

text: Generic

characteristic: cultivar

text: Cucumber BAC library HindIII partial digest synthesized by Keygene, Wageningen, Holland

text: Cucumis sativus

text: Cucumber TMG1 BAC Library

text: BAC


text: Max Planck Institute for Developmental Biology

text: live

characteristic: INSDC status

text: Generic

text: BIOS&T, 8fold coverage, 164KB average insert size

text: adult

characteristic: dev-stage

text: E.coli DH 10B

characteristic: lab-host

text: whole fish

characteristic: organ

text: Poecilia reticulata

text: Hind III

text: Hind III

text: male

text: Cumana(Ve)

text: Guppy pIndigoBAC-5 genomic

text: BAC


text: Centro de Astrobiologia INTA-CSIC

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Total DNA from L. infantum was partially digested with Sau3AI. Fragments of size 2-11 Kbp were purified through 10-40% sucrose gradient isopicnic centrifugation and ligated into BamHI-digested pUC18.

characteristic: description

text: Stationary phase promastigotes

text: Escherichia coli DH10B

text: Leishmania infantum

text: BamHI

text: Plasmid


text: Purdue University

text: live

text: Generic

text: DNA sequence obtained from PCR products amplified from W22 genomic DNA using gene target - specific primers. The PCR product was sequenced from both the 5' and 3' ends.

text: Zea mays

characteristic: organism

text: Maize TILLING Project Target Sequences CW_W22 population

characteristic: title


characteristic: External Id

text: Department of Primary Industries

text: live

text: Generic

text: Albugo candida

text: Race 9

characteristic: strain

text: zoosporangia

characteristic: tissue-type

text: Albugo shotgun library


text: Facultad de Veterinaria (Universidad Complutense de Madrid)

text: live

text: Generic

text: PCR reactions were performed with genomic DNA, SNPs were detected with SSCP and products were analysed by DNA sequencing.

text: Alectoris chukar

text: Alectoris chukar sequenced library


text: live

text: Generic

text: Alectoris rufa

text: Alectoris rufa sequenced library


text: NOAA Fisheries

text: live

text: Generic

text: Green turtle microsatellite clone sequences without STRs

text: Chelonia mydas

text: Green turtle msat library


text: Institute of Biology, Leiden University

text: live

text: Generic

text: PCR product directly amplified from rice genomic DNA of Tos17 insertion rice line NC27280

text: Oryza sativa Japonica Group

text: Flanking sequence of Tos17 insertion in rice line NC2728


text: Institute of Biology, Leiden University

text: live

text: Generic

text: PCR product directly amplified from rice genomic DNA of Tos17 insertion rice line ND0051

text: Oryza sativa Japonica Group

text: Flanking sequence of Tos17 insertion in rice line ND0051


text: Institute of Botany, University of Kiel

text: live

text: Generic

text: Carina

text: etiolated seedling

text: Hordeum vulgare subsp. vulgare

text: vulgare

characteristic: sub species

text: primary foliage leaf

text: Barley genomic Lambda FIXIII Library, Stratagene, Heidelberg, Germany


text: Institute of Microbiology, Chinese Academy of Sciences

characteristic: INSDC center name

text: live

text: Generic

text: Fosmid ends of clones from a South China sea marine sediment microbial fosmid library. The sampling site information: latitude: 17:33:35; longitude: 110:22:47; depth: 778.5m.

text: E.coli EPI300

text: marine sediment metagenome

text: BD7-2 metagenome IMCAS-F003

text: CopyControl pCC2FOS

characteristic: vector


text: University of Pennsylvania School of Medicine

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Homo sapiens

text: HERV Integration Site Library


text: Kirin Brewery Co. Ltd

text: live

characteristic: INSDC status

text: Generic

text: Saccharomyces pastorianus


text: Pastoral Genomics, c/o ViaLactia Biosciences (NZ) Ltd

text: live

text: Generic

text: Bronsyn

characteristic: cultivar

text: The library was constructed using GeneThresher technology which was developed to take advantage of differential methylation patterns by filtering genomic shotgun libraries to exclude methylated sequences (Rabinowicz et al., Nat. Genet.23:305-308,1999).

text: Lolium perenne

text: Perennial ryegrass gene-enriched genomic shotgun library

text: pBluescriptIISK+


text: UMR1090, Inra

text: live

text: Generic

text: specific_host: Scaphoideus titanus. country: France:Landes. collection_date: 1970. collected_by: Antoine Caudwell.

characteristic: isolate

text: Catharanthus roseus

characteristic: lab-host

text: Candidatus Phytoplasma vitis

text: Candidatus Phytoplasma vitis isolate FD70


text: Fudan University

text: live

text: Generic

characteristic: NCBI submission model

text: We have generated a number of mutant mouse lines with PB transposon insertional mutagenesis. Several PB transposon vectors have been used. This plasmid vector PB[Act-RFP] is one of them, which has been published already: Ding S, Wu X, Li G, Han M, Zhuang Y, Xu T. Efficient transposition of the piggyBac (PB) transposon in mammalian cells and mice. Cell. 2005 Aug 12;122(3):473-83. Epub 2005 Jul. 21.

text: Mus musculus

text: Mus musculus PB transposon, insertional mutagenesis

text: Plasmid

text: PB[Act-RFP]


text: Fudan University

text: live

text: Generic

characteristic: NCBI submission package

text: We have generated a number of mutant mouse lines with PB transposon insertional mutagenesis. Several PB transposon vectors have been used. This plasmid vector PB[Trap3F] is one of them, which has not been published yet.

text: Mus musculus

text: Mus musculus PB transposon, insertional mutagenesis, PB[Trap3F]

text: Plasmid


text: Fudan University

text: live

text: Generic

text: We have generated a number of mutant mouse lines with PB transposon insertional mutagenesis. Several PB transposon vectors have been used. This plasmid vector PB[Trap6R] is one of them, which has not been published yet.

characteristic: description

text: Mus musculus

text: Mus musculus PB transposon, insertional mutagenesis, PB[Trap6R]

text: Plasmid


text: Medical School of Xi'an Jiaotong University

text: live

text: Generic

text: adult mite

characteristic: dev-stage

text: Demodex folliculorum

characteristic: organism

text: Demodex folliculorum adult mite


characteristic: External Id

text: live

text: Generic

text: adult mite

text: Demodex brevis

text: Demodex brevis adult mite

characteristic: title


text: Lawrence Berkeley National Laboratory

text: live

text: Generic

text: High molecular weight genomic DNA from mixed-sex adult flies was prepared in agarose blocks from the isogenized y1; cn1 bw1 sp1 strain used to produce the reference D. melanogaster genome sequence. Genomic DNA was partially digested with MboI, size fractionated, and cloned into the BamHI site of the BAC vector attB-P[acman]-CmR-BW. The mean insert size of the resulting clones is 83.3 kb (+/- 21.5 kb).

text: Drosophila melanogaster

text: MboI

text: MboI

text: iso-1 (y1; cn1 bw1 sp1)

characteristic: strain

text: attB-P[acman]-CmR-BW


text: Lawrence Berkeley National Laboratory

text: live

text: Generic

text: High molecular weight genomic DNA from mixed-sex adult flies was prepared in agarose blocks from the isogenized y1; cn1 bw1 sp1 strain used to produce the reference D. melanogaster genome sequence. Genomic DNA was partially digested with MboI, size fractionated, and cloned into the BamHI site of the BAC vector attB-P[acman]-CmR-BW. The mean insert size of the resulting clones is 21.0 kb (+/- 4.0 kb).

text: Drosophila melanogaster

text: MboI

text: MboI

text: iso-1 (y1; cn1 bw1 sp1)

text: attB-P[acman]-CmR-BW


text: Michigan State University

text: live

text: Generic

text: Nici

text: Meleagris gallopavo

text: Female

characteristic: sex

text: Partially inbred commercial line, Nicholas Turkey Breeding Farms

text: BAC


text: International Atomic Energy Agency (IAEA)

characteristic: INSDC center name

text: live

text: Generic

text: Whole genome partial-digest Sau3A library.

text: Anopheles albimanus

text: BamHI

text: BamHI

text: female and male mixture

text: laboratory colonies

text: whole animal

characteristic: tissue-type

text: Anopheles albimanus microsatellite I

text: Plasmid

text: pUC derived


text: College of Bioresource Sciences, Nihon University

characteristic: INSDC first public

text: live

text: Generic

text: type strain of Nosema bombycis. The sequence was determined with genome subtracted DNA clones.

text: spore

text: Bombyx mori

text: Nosema bombycis

text: Nosema bombycis NIS-001 spore


text: European Conditional Mouse Mutagenesis Program (EUCOMM)

characteristic: INSDC last update

text: live

text: Generic

text: Embryonic Stem Cell

characteristic: cell type

characteristic: cell-line

text: Random gene traps from the EUCOMM project with conditional retroviral gene trap vectors.

text: Mus musculus

text: male

text: 129P2 (formerly 129/OlaHsd)

text: EUCOMM random gene traps


text: ZMBP Plant Physiology, Tuebingen University

text: live

characteristic: INSDC status

text: Generic

text: Genomic DNA of Biscutella didyma was extracted from Biscutella didyma leaves. Random priming amplification was performed and fragments were subsequently cloned into pCR2-Topo (Invitrogen). Plasmid inserts were entirely sequenced. Primer sequences have been removed prior submission. Sequence has been verified by re-PCR from genomic DNA.

text: Biscutella didyma

text: leaves

text: Biscutella didyma Pooled genomic DNA

characteristic: vector


text: Lawrence Berkeley National Laboratory

text: live

text: Generic

characteristic: NCBI submission model

text: These are the DNA sequences flanking the insertion sites of a collection of P-element transposon insertion mutants of Drosophila melanogaster that were produced by GenExel, Inc. The transposon mutator is the synthetic construct P{EP} (Rorth (1996) Proc. Natl. Acad. Sci. USA 93:12418-12422; FlyBase ID: FBtp0001317). Sequences flanking the insertion sites of mutants were amplified by inverse PCR and sequenced initially by GenExel, Inc. and subsequently at the Lawrence Berkeley National Laboratory using an unpublished modification of the protocol of Bellen et al. 2004 (Genetics 167: 761-781; FlyBase ID: FBrf0179132). The protocols used at the LBNL may be downloaded from the Web site at: http://flypush.imgen.bcm.tmc.edu/pscreen/.

text: Drosophila melanogaster

text: GenExel P-element insertion flanking sequences


text: Department of Agronomy

text: live

text: Generic

characteristic: NCBI submission package

text: Protocorm-like bodies from Cymbidium hybridium.

text: Cymbidium hybrid cultivar

text: Protocorm-like bodies

text: Orchids PLBs Library


text: Embrapa Soja

text: live

text: Generic

text: Bradyrhizobium japonicum

text: Bradyrhizobium japonicum RDA Library


text: Kumamoto University

text: live

text: Generic

text: Embryonic stem cell (feeder free)

text: ES cells KTPU8 (TT2 sub line)

text: country: Japan

characteristic: description

text: Mus musculus

text: male

text: EGTC Gene Trap Library, KTPU8, pU-21T


text: Universidade do Algarve

text: live

text: Generic

characteristic: cultivar

text: Insert size: ~105 Genome coverage: 14

text: Brassica oleracea

text: HindIII

text: BAC


text: Universidade do Algarve

text: live

text: Generic

text: Badger Inbred

text: Insert size: ~90 Genome coverage: 5.6

text: Brassica oleracea

characteristic: organism

text: HindIII

text: BOCIG

text: BAC


characteristic: External Id

text: Scion

text: live

text: Generic

text: PCR products were excised from agarose gel, ligated into pGEM-T and sequenced using M-13F and M-13R

characteristic: isolate

text: Fusarium circinatum

text: Gibberella circinata conserved RAPD PCR products

text: plasmid

text: pGEM-T


text: Kumamoto University

text: live

text: Generic

text: Embryonic stem cell (feeder free)

text: ES cells KTPU8 (TT2 sub line)

text: Sequence tag generated by 5'RACE of mRNA from gene trap ES cell line. country: Japan.

text: Mus musculus

text: male

characteristic: strain

text: EGTC Gene Trap Library, KTPU8, pU-21W

characteristic: title


text: John Innes Centre

characteristic: INSDC center name

text: live

text: Generic

text: 'Tapidor'

text: Brassica napus

text: BamHI

text: Leaves

text: Brassica napus BAC library (JBnB)


text: University of Leeds

text: live

text: Generic

text: Toxoplasma gondii strain from Uganda

text: Toxoplasma gondii

text: Toxoplasma gondii strain TgCkUg1 PCR product


text: University of Leeds

text: live

text: Generic

text: Toxoplasma gondii strain from Uganda

text: Toxoplasma gondii

text: Toxoplasma gondii strain TgCkUg3 PCR product


text: University of Leeds

text: live

text: Generic

text: Toxoplasma gondii strain from Uganda

text: Toxoplasma gondii

text: Toxoplasma gondii strain TgCkUg5 PCR product


text: University of Leeds

text: live

text: Generic

text: Toxoplasma gondii strain from Uganda

text: Toxoplasma gondii

text: Toxoplasma gondii strain TgCkUg6 PCR product


text: University of Leeds

text: live

text: Generic

text: Toxoplasma gondii strain from Uganda

text: Toxoplasma gondii

text: Toxoplasma gondii strain TgCkUg7 PCR product


text: University of Leeds

text: live

text: Generic

text: Toxoplasma gondii strain from Uganda

text: Toxoplasma gondii

text: Toxoplasma gondii strain TgCkUg8 PCR product


text: University of Leeds

text: live

text: Generic

text: Toxoplasma gondii strain from Uganda

text: Toxoplasma gondii

text: Toxoplasma gondii strain TgCkUg9 PCR product


text: University of California, Davis, and The Institute for Genomic Research

text: live

text: Generic

text: Tifrunner

text: Peanut (Arachis hypogea) cultivar Tifrunner was grown under greenhouse conditions for 6 weeks and transferred to continuous darkness for 2 days prior to use. High molecular weight DNA was isolated and random sheared DNA was size selected based on proprietary methods from Lucigen Corp. Large size DNA fragments were ligated in vector pSMART-BAC and transformed by electroporation in to Epicenter's E. coli 10G (DH10B) BAC-Optimized cells. Approximately 110,000 clones were obtained, with most inserts averaging 100 kbp.

text: Leaves

characteristic: organ

text: Arachis hypogaea

text: Sheared DNA

text: pSMART-BAC (random shear clones)


text: University of Delhi

characteristic: INSDC first public

text: live

text: Generic

text: Genomic DNA randomly digested with HindIII restriction enzyme and cloned in pBSK Bluscript

text: Adult

characteristic: dev-stage

text: Icerya aegyptiaca

text: Hermaphrodite

characteristic: sex

text: Whole organism

characteristic: tissue-type

text: Mealybug gDNA Library

text: pBSK Bluscript


text: Max Planck Institut fuer Zuechtungsforschu

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: seedling

text: Antirrhinum majus

text: Antirrhinum majus 165E BAC clone library


text: Universidade Federal do Triangulo Mineiro

text: live

text: Generic

characteristic: NCBI submission model

text: Genomic DNA of P07 strain of Trypanosoma rangeli was digested with BamHI and BglII restriction endonucleases and fragments of 2 to 4 kb were ligated into pUC18 linearized by digestion with BamHI.

text: Epimastigote

text: Field isolate

text: Trypanosoma rangeli

text: Trypanosoma rangeli P07 Epimastigote


text: Mie University, Faculty of Bioresources

text: live

text: Generic

characteristic: NCBI submission package

text: country=China: South China Sea ; isolation_source: sea bass

text: Sea bass iridovirus

text: Sea bass iridovirus DNA


text: Saga University

text: live

text: Generic

text: isolation_source: Rice ; country: Japan: Aichi

characteristic: description

text: Pyricularia grisea

text: Magnaporthe grisea isolate 84R-62B


text: The University of Georgia

text: live

text: Generic

characteristic: cultivar

text: 6-8 weeks

characteristic: lab-host

text: Zea mays

text: EcoRI

text: EcoRI

text: immature ear

text: ZMMBHa

characteristic: vector


characteristic: External Id

text: The University of Georgia

text: live

text: Generic

text: 6-8 weeks

text: Zea mays

characteristic: organism

text: EcoRI

text: EcoRI

text: immature ear

text: ZMMBHb


text: The University of Georgia

text: live

text: Generic

text: 6-8 weeks

text: Zea mays

text: EcoRI

text: EcoRI

text: immature ear

text: ZMMBHd

characteristic: title


text: The University of Georgia

characteristic: INSDC center name

text: live

text: Generic

text: 6-8 weeks

text: Zea mays

text: EcoRI

text: EcoRI

text: immature ear

text: ZMMBHe


text: The University of Georgia

text: live

text: Generic

text: 6-8 weeks

text: Zea mays

text: EcoRI

text: EcoRI

text: immature ear

text: ZMMBHf


text: The University of Georgia

text: live

text: Generic

text: 6-8 weeks

text: Zea mays

text: EcoRI

text: EcoRI

text: immature ear

text: ZMMBHg


text: The University of Georgia

text: live

text: Generic

text: 6-8 weeks

text: Zea mays

text: EcoRI

text: EcoRI

text: immature ear

text: ZMMBHh


text: The University of Georgia

text: live

text: Generic

text: 6-8 weeks

text: Zea mays

text: EcoRI

text: EcoRI

text: immature ear

text: ZMMBHi


text: The University of Georgia

text: live

text: Generic

text: 6-8 weeks

text: Zea mays

text: EcoRI

text: EcoRI

text: immature ear

text: ZMMBHj


text: The University of Georgia

text: live

text: Generic

text: 6-8 weeks

text: Zea mays

text: EcoRI

text: EcoRI

text: immature ear

text: ZMMBHk


text: The University of Arizona

text: live

text: Generic

text: DH10B T1 phage resistant

text: Zea mays

text: SalI

text: SalI

text: immature ears

text: ZMMBLa


text: The University of Arizona

text: live

text: Generic

text: DH10B T1 phage resistant

text: Zea mays

text: Hpa II

text: Hpa II

text: immature ears

text: ZMMBLc


text: The University of Georgia

text: live

text: Generic

text: 6-8 weeks

text: Zea mays

text: EcoRI

text: EcoRI

text: immature ear

text: ZMMBHc


text: Chulalongkorn University

text: live

text: Generic

text: isolation_source: Fish

text: Vibrio anguillarum

characteristic: strain

text: Listonella anguillarum plasmid library


text: Chulalongkorn University

characteristic: INSDC first public

text: live

text: Generic

text: Fish

text: Vibrio anguillarum

text: Listonella anguillarum Cosmid library


text: University of Arizona

text: live

text: Generic

text: Methyl Spanning Linker Library (HpaII, BAC)

text: DH10B T1 phage resistant

text: Zea mays

text: Hpa II

text: Hpa II

text: immature ears

text: ZMMBLb


text: University of Arizona

text: live

text: Generic

text: Methyl Spanning Linker Library (SalI, BAC)

text: DH10B T1 phage resistant

text: Zea mays

text: Sal I

text: Sal I

text: immature ears

text: ZMMBLd


text: University of Arizona

text: live

text: Generic

text: DH10B T1 phage resistant

text: Zea mays

text: Hpa II

text: Hpa II

text: immature ears

text: ZMMBLe


text: University of Arizona

text: live

text: Generic

text: DH10B T1 phage resistant

text: Zea mays

text: Sal I

text: Sal I

text: immature ears

text: ZMMBLf


text: University of Arizona

text: live

text: Generic

text: DH10B T1 phage resistant

text: Zea mays

text: Sal I

text: Sal I

text: immature ears

text: ZMMBLh


text: University of Arizona

text: live

text: Generic

text: DH10B T1 phage resistant

text: Zea mays

text: Hpa II

text: Hpa II

text: immature ears

text: ZMMBLi


text: University of Arizona

text: live

text: Generic

text: DH10B T1 phage resistant

text: Zea mays

text: Hpa II

text: Hpa II

text: immature ears

text: ZMMBLj


text: University of Arizona

text: live

text: Generic

text: Methyl Spanning Linker Library (HpaII, fosmid)

text: DH10B T1 phage resistant

text: Zea mays

text: Hpa II

text: Hpa II

text: immature ears

text: ZMMBLz


text: Forestry and Forest Products Research Institute

text: live

text: Generic

text: Beauveria bassiana

text: Bb_F263 RAPD

text: p3T vector


text: Purdue University

text: live

text: Generic

text: 6-8 weeks

characteristic: dev-stage

text: Zea mays

text: Eco RI

text: Eco RI

text: Immature ear

text: ZMMBHl

text: Plasmid


text: Purdue University

text: live

text: Generic

text: 6-8 weeks

text: Zea mays

text: Eco RI

text: Eco RI

text: Immature ear

text: ZMMBHm

text: Plasmid


text: Purdue University

text: live

text: Generic

text: 6-8 weeks

text: Zea mays

text: Eco RI

text: Eco RI

text: Immature ear

text: ZMMBHn

text: Plasmid


text: Purdue University

text: live

text: Generic

text: 6-8 weeks

text: Zea mays

text: Eco RI

text: Eco RI

text: Immature ear

text: ZMMBHo

text: Plasmid


text: Purdue University

text: live

text: Generic

text: 6-8 weeks

text: Zea mays

text: Eco RI

text: Eco RI

text: Immature ear

text: ZMMBHp

text: Plasmid


text: Purdue University

text: live

text: Generic

text: 6-8 weeks

text: Zea mays

text: Eco RI

text: Eco RI

text: Immature ear

text: ZMMBHq

text: Plasmid


text: Purdue University

text: live

text: Generic

text: 6-8 weeks

text: Zea mays

text: Eco RI

text: Eco RI

text: Immature ear

text: ZMMBHr

text: Plasmid


text: Purdue University

text: live

text: Generic

text: 6-8 weeks

text: Zea mays

text: Eco RI

text: Eco RI

text: Immature ear

text: ZMMBHs

text: Plasmid


text: ECORC, Agriculture and Agri-Food Canada

text: live

text: Generic

text: Genomic clones for DArT analysis generated from complexity-reduced representations of pooled DNA samples from diverse oat cultivars

text: Avena sativa


text: Children's Hospital Oakland Research Institute

text: live

text: Generic

text: PCR products from genomic DNA

text: Hylobates agilis

text: Hylobates agilis genomic DNA


text: live

text: Generic

text: PCR products from genomic DNA

text: Hoolock leuconedys

text: Hoolock leuconedys genomic DNA


text: live

text: Generic

text: PCR products from genomic DNA

text: Hylobates moloch

text: Hylobates moloch genomic DNA


text: live

text: Generic

text: PCR products from genomic DNA

text: Symphalangus syndactylus

text: Symphalangus syndactylus genomic DNA


text: Eccles Institue of Human Genetics

text: live

text: Generic

text: Homo sapiens

text: Homo sapiens Genomic DNA (Xing)


text: Eccles Institue of Human Genetics

text: live

text: Generic

text: Pan troglodytes

text: Pan troglodytes Genomic DNA (Xing)


text: National Institute of Animal Science, RDA

text: live

text: Generic

text: KNP BAC library represents seven genome equivalents. Average insert size is about 125 kb.

text: Sus scrofa

text: EcoRI

text: EcoRI

text: male

characteristic: sex

text: Korean Native Pig

text: White Blood Cell

text: KNP_BAC: Korean Native Pig BAC Library

text: BAC


text: University of Turin

text: live

text: Generic

text: Amplification of genomic DNA with SCAR primers

text: Monilinia fructicola

text: MF


text: Institute of Microbiology, Chinese Academy of Sciences

text: live

text: Generic

text: Fosmid library obtained from marine sediment community DNAs, sampled from South China Sea. Collected March 3th, 2006.

text: marine sediment metagenome

text: A11_Marine sediment metagenomic fosmid library

text: fosmid


text: Blood Systems Research Institute

text: live

text: Generic

text: This study was performed using random amplification of partially purified viral nucleic acid from the stool of Pakistani children with acute flaccid paralysis (AFP). Samples are from 35 AFP cases and 6 healthy contacts.

text: human gut metagenome

text: Viral Genomic Survey of Stool from South East Asian Children with Acute Flaccid Paralysis


text: Aarhus University - Faculty of Agriultural Sciences

text: live

text: Generic

text: adult

text: Sus scrofa

text: male

text: Duroc

text: muscle

characteristic: tissue-type

text: Duroc Pig genomic


text: Nanjing Normal University

characteristic: INSDC last update

text: live

text: Generic

text: Neophocaena phocaenoides

text: Finless porpoise genomic library


text: Emory University

text: live

characteristic: INSDC status

text: Generic

text: The white-throated sparrow (Zonotrichia albicollis) CHORI-264 BAC was library constructed by Pieter J. de Jong, Boudewijn ten Hallers, and Maxim Koriabine at CHORI. Additional information on the library is available at: http://bacpac.chori.org/library.php?id=469.

text: adult

text: Zonotrichia albicollis

text: female

text: kidney


text: Emory University

text: live

text: Generic

characteristic: NCBI submission model

text: The prairie vole (Microtus ochrogaster) CHORI-232 BAC was library constructed by Pieter J. de Jong and Boudewijn ten Hallers at CHORI. Additional information on the library is available at: http://bacpac.chori.org/library.php?id=481.

text: adult

text: Microtus ochrogaster

text: male

text: kidney


text: University of Pennsylvania School of Medicine

text: live

text: Generic

characteristic: NCBI submission package

text: Homo sapiens

text: Carl June Integration Site Library


text: The University of Arizona

text: live

text: Generic

text: Bd21 cycle 9

text: 2-3 weeks

text: DH10B T1 phage resistant

text: Brachypodium distachyon

text: HindIII

text: HindIII

text: young leaves


text: The University of Arizona

text: live

text: Generic

text: 2-3 weeks

text: DH10B T1 phage resistant

text: Brachypodium distachyon

text: EcoRI

text: EcoRI

text: young leaves


text: Scripps Florida

text: live

text: Generic

text: DNA sample from tail prep

characteristic: description

text: Mus musculus

text: male

text: BALB/cByJ

text: tail

text: Mouse strain BALB/cByJ male tail DNA


text: Scripps Florida

text: live

text: Generic

text: DNA sample from tail prep

text: Mus musculus

text: male

text: BTBR T+ tf/J

text: tail

text: Mouse strain BTBR T+ tf/J male tail DNA


text: Scripps Florida

text: live

text: Generic

text: DNA sample from tail prep

text: Mus musculus

text: male

text: tail

text: Mouse strain C57BL/6J male tail DNA


text: Scripps Florida

text: live

text: Generic

text: DNA sample from tail prep

text: Mus musculus

text: male

text: tail

text: Mouse strain DBA/2J male tail DNA


text: Scripps Florida

text: live

text: Generic

text: DNA sample from tail prep

text: Mus musculus

text: male

text: FVB/NJ

text: tail

text: Mouse strain FVB/NJ male tail DNA


text: Scripps Florida

text: live

text: Generic

text: DNA sample from tail prep

text: Mus musculus

text: male

text: MA/ MyJ

text: tail

text: Mouse strain MA/ MyJ male tail DNA


text: Scripps Florida

text: live

text: Generic

text: DNA sample from tail prep

text: Mus musculus

text: male

text: NZW/LacJ

text: tail

text: Mouse strain NZW/LacJ male tail DNA


text: Scripps Florida

text: live

text: Generic

text: DNA sample from tail prep

text: Mus musculus

text: male

text: RIIIS/J

text: tail

text: Mouse strain RIIIS/J male tail DNA


text: J. Craig Venter Institute

text: live

text: Generic

text: Brassica oleracea BAC library BOT01. BACs prepared from partial HindIII digest of genomic DNA, by B. Yuksel and A. H. Paterson, Plant Genome Mapping Lab, Univ. GA.

text: Brassica oleracea

text: TO 1434

text: young seedlings


text: Institute for Bee Research

text: live

text: Generic

text: Suppressive subtraction hybridization (SSH) was carried out using the PCR-Select Bacterial Genome Subtraction Kit (Clontech PCR-Select Bacterial Genome Subtraction Kit, BD Clontech) as recommended by the supplier. Secondary PCR products generated in the library were cloned into pCR2.1TOPO vector (Invitrogen) following the manufacturer's instructions and transformed into TOP10 cells (Invitrogen).

text: TOP10 E. coli

text: Paenibacillus larvae

text: P.larvae SSH library

text: Plasmid


text: University of Southern Denmark

text: live

text: Generic

text: Total genomic DNA cleaved to completion with BamHI and cloned into pUC18.

text: Adult

text: Tail

characteristic: organ

text: Oedura marmorata

text: BamHI

text: Male

text: Australian Velvet Gecko, pUC18 library

text: Plasmid


text: Eugeniks Genetic Research Institute

text: live

text: Generic

text: Leukocytes

characteristic: cell type

text: Homo sapiens

text: Human Leukocyte RAPD


text: East China University of Science and Technology

text: live

text: Generic

text: Separation of Highly Repetitive DNA for Construction HRCot Library of Equus asinus

text: Equus asinus

text: Equus asinus highly repetitive cot library

text: Plasmid

text: pGEM-T

characteristic: vector


characteristic: External Id

text: INRA/CNRS, UMRGV

text: live

text: Generic

text: Wassillewskija

characteristic: cultivar

text: Arabidopsis thaliana

text: Arabidopsis thaliana ecotype Wassillewskija T-DNA insertion lines


text: University of Georgia

text: live

text: Generic

text: Nuclear DNA, Sheared to 3-4kb, end repaired, PCR-4 Topo Vector.

text: Mature - flowering

text: Striga asiatica

characteristic: organism

text: hermaphrodite

text: Tanzania, collected from Sorghum host

text: above ground portion, inflorescence

characteristic: title


text: University of Georgia

characteristic: INSDC center name

text: live

text: Generic

text: Mature - flowering

text: Striga asiatica

text: hermaphrodite

text: Zimbabwe, collected from maize host

characteristic: strain

text: above ground portion, inflorescence


text: University of Georgia

characteristic: INSDC first public

text: live

text: Generic

text: Mature - flowering

characteristic: dev-stage

text: Striga forbesii

text: hermaphrodite

text: Africa, collected from maize host

text: above ground portion, inflorescence


text: University of Georgia

text: live

text: Generic

text: Mature - flowering

text: Striga hermonthica

text: hermaphrodite

characteristic: sex

text: Sudan, collected from Sorghum host

text: above ground portion, inflorescence

characteristic: tissue-type


text: University of Georgia

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Mature - flowering

text: Striga hermonthica

text: hermaphrodite

text: Nigeria, collected from maize host

text: above ground portion, inflorescence


text: University of Georgia

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Mature - flowering

text: Striga aspera

text: hermaphrodite

text: Mali, collected from Sorghum host

text: above ground portion, inflorescence


text: California Institute for Medical Research

text: live

text: Generic

text: Yeasts

text: In brief, after hydrodynamic shearing, blunt-ended gDNA inserts for cloning were maximized using T4 DNA polymerase. The reaction contained 3 ug sheared gDNA, 5 ul 10x T4 DNA Polymerase Reaction Buffer, 100 uM dNTPs, 5 units of T4 DNA Polymerase per ug of gDNA, 0.1 mg/ml bovine serum albumin (BSA) (Promega) and molecular biology grade water (Sigma-Aldrich) in a 50 ul total reaction volume. Reactions were incubated at 37oC for 5 minutes followed by a heat-inactivation at 75oC for 10 minutes. The blunt-ended fragments were purified using a QIAquick Nucleotide Removal Kit (QIAGEN Inc., Valencia, CA) according to the manufacturer's instructions. Double-stranded adaptors were assembled by mixing equimolar concentrations of oligonucleotides (5'pTGAGTCACCAAAC3' and 5'pGTGACTCA3'), and incubation at 50oC for 10 minutes, followed by cooling to ambient temperature (10 minutes) and further incubation at 16oC overnight. Thereafter, the adaptor solution was kept on ice during usage or stored at -20oC to maintain annealed strands. The adaptor to insert ligation reaction was assembled as follows: 1 ug of blunt-ended sheared gDNA, 5 ul of 10X T4 DNA Ligation Reaction Buffer (Promega), 40 units of 20 U/ul T4 DNA ligase (Promega), 100 uM adaptor and molecular biology grade water (Sigma-Aldrich) was added to a final reaction volume of 50 ul. Linker oligonucleotides (5'pAATTGTTTG3') were ligated to the EcoRI 5' overhangs of the phage vector as follows: 1.0 ug of Lambda ZAP II DNA, 100 uM linker oligonucleotide, 10 units of 20 U/ul T4 DNA Ligase, 1ul 10X T4 DNA Ligase Reaction Buffer and nuclease-free water to a 10 ul total reaction volume. All ligation reactions were incubated at 12oC overnight. Excess linkers, adaptors or adaptor dimers were removed using one CHROMA SPINTM + TE-400 Column (BD Biosciences, San Jose, CA) followed by six ultrafiltration runs, using one Microcon filter per three runs (Microcon YM-100 Centrifugal Filter Devices, Millipore Corp., Billerica, MA). The purified phage vector DNA with ligated linkers and sheared gDNA with ligated adaptors were combined and dried in a Speedvac Concentrator (Savant Instruments Inc., Farmingdale, NY). Final ligation was done in the tube containing the dried DNA and consisted of 0.5 ul 10x T4 DNA Ligase Reaction Buffer, 10 units of 20 U/ul of T4 DNA ligase (Promega) and nuclease-free water (Sigma-Aldrich) to a final volume of 5 ul. This reaction was incubated at 16oC overnight. Ligation products were packaged in phage capsids using Gigapack III Gold Packaging Extract (Stratagene, La Jolla, CA) and plated with the bacterial host strain for titer determination and phage plaques containing clones for isolation per the product manual. Individual plaques were picked using sterile Pasteur pipettes and stored in SM Buffer (Qbiogene, Inc., Irvine, CA) containing 7% DMSO (Sigma-Aldrich, St. Louis, MO) in sealed 96-deep well plates at -80oC. All oligonucleotides used in this study were purchased from Operon Biotechnologies, Inc. (Huntsville, AL).

characteristic: description

text: Pb01 (ATCC: MYA-826)

characteristic: isolate

text: Paracoccidioides brasiliensis

text: Paracoccidioides brasiliensis genomic library

text: Phage

text: Lambda ZAP II


text: University of Delhi

text: live

text: Generic

text: Genomic DNA randomly digested with Eco RI restriction enzyme and cloned in pBSK Bluscript

text: Adult

text: Phenacoccus solenopsis

text: EcoRI

text: Hermaphrodite

text: Whole organism

text: Phenacoccus solenopsis gDNA Library

text: pBSK Bluscript

characteristic: vector


characteristic: External Id

text: J. Craig Venter Institute

text: live

text: Generic

text: BACs prepared from partial BamHI digest of genomic DNA, by Aretha Fiebig and Daphne Preuss, Dept. of Molecular Genetics & Cell Biology, University of Chicago

text: Sisymbrium irio

text: ABRC Stock: CS22563

text: Rosette leaves

text: Sisymbrium irio BAC library SIR


text: Graduate School of Medicine, Kyoto University

text: live

text: Generic

text: peripheral lymphocytes

characteristic: cell type

text: Rattus norvegicus

text: SacI

text: male

text: LE/Stm

text: Rattus norvegicus LE/Stm whole genome BAC library, RNB2


text: UMR CNRS 6023

text: live

text: Generic

text: the bacterial community in a mesotrophic lake (Lac du Bourget - France)

text: freshwater metagenome

characteristic: organism

text: METAPROC

text: Fosmid


text: Iowa State University

text: live

text: Generic

characteristic: cultivar

text: Transposition events of an endogenous, single-copy Dissociation (Ds) element were selected by a two step process. First, kernels were scored for Ds excision from a donor locus. Then to detect Ds reinsertion, genomic DNA was extracted from F2 segregants that lacked Ac and was analyzed by gel blots with methylation sensitive restriction enzymes and a Ds probe. DNA flanking one side of the transposed Ds was recovered by IPCR, gel-purified and sequenced directly with nested primers from one or both sides of the fragment. A few fragments required additional PCR or cloning steps to generate sequence. The obtained sequence was processed to remove low quality, vector and Ds-derived sequences, to assemble reads into contigs, and to correlate the sequence with information derived from the Southern blot and IPCR steps. For additional details see the project web page at http://plantgdb.org/prj/AcDsTagging/index.php.

text: seedling

text: Zea mays

text: leaf

text: Maize transposon insertions from ISU and BTI

characteristic: title


text: INRA

characteristic: INSDC center name

text: live

text: Generic

text: vegetative stage

text: DH5 alpha

characteristic: lab-host

text: Agaricus bisporus

characteristic: strain

text: mycelium

text: Agaricus bisporus partial genomic DNA library


text: CIRAD

characteristic: INSDC first public

text: live

text: Generic

text: Genomic clones for DArT analysis generated from MITE complexity-reduced representations of pooled DNA samples from diverse sorghum cultivars

text: Sorghum bicolor

text: sorghum MITE DArT library

text: Plasmid

text: PCR 2.1 topo


text: CIRAD

text: live

text: Generic

text: Genomic clones for DArT analysis generated from PSTI-BanII complexity-reduced representations of pooled DNA samples from diverse sorghum cultivars

text: Sorghum bicolor

text: sorghum PSTI-BanII DArT library

text: Plasmid

text: PCR 2.1 topo


text: National Institute of Agriculture Science

text: live

text: Generic

text: Misuzudaizu

text: Glycine max

text: Glycine max cv. Misuzudaizu DNA


text: live

text: Generic

text: Glycine max

text: Glycine max DNA (Watanabe S)


text: University of Southern Denmark

text: live

text: Generic

text: Total genomic DNA partially cleaved with Sau3A and cloned into plasmid pUC18.

text: Young adult

characteristic: dev-stage

text: Whole organism

characteristic: organ

text: Dendrobates tinctorius

text: Oranjegeberg

text: Blue poison-arrow frog, Sau3A, pUC18 library

text: Plasmid


text: University of Southern Denmark

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Total genomic DNA cleaved to completion with BamHI and cloned into plasmid pUC18.

text: Young adult

text: Whole organism

text: Dendrobates tinctorius

text: BamHI

text: Oranjegeberg

text: Blue poison-arrow frog, BamHI, pUC18 library

text: Plasmid


text: German Genetrap Consortium (GGTC)

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Embryonic stem cell

text: ES cells 129P2 (formerly 129/Ola)

characteristic: cell-line

text: Mus musculus

text: Male

characteristic: sex

text: 129 Sv

text: GGTC Gene Trap Library GV34C07

text: Retroviral insertion mutagen


characteristic: External Id

text: The Ohio State University, OARDC

text: live

text: Generic

text: Solanum lycopersicum

text: Tomato Genome SNP, InDel Search Library, cultivar FL7600


text: The Ohio State University, OARDC

text: live

text: Generic

text: Solanum lycopersicum

text: Tomato Genome SNP, InDel Search Library, cultivar OH9242


text: The Ohio State University, OARDC

text: live

text: Generic

text: Solanum lycopersicum

text: Tomato Genome SNP, InDel Search Library, cultivar PI114490


text: National Institute of Horticultural & Herbal Science, RDA

text: live

text: Generic

text: Yunpoong

text: Ginseng genomic DNA was digested with SpeI which is compatible with the XbaI.

characteristic: description

text: 3 year old leaves

text: E. coli XL-1 Blue MRF'

text: Panax ginseng

text: XbaI

text: leaf

characteristic: tissue-type

text: Unfiltered ginseng genomic library (UFG)

text: Plasmid

characteristic: vector


text: University of Oxford

text: live

text: Generic

text: sporidia

characteristic: cell type

text: specific-host=Silene latifolia; country=France; substrain=11.4.1; mating-type=A2

text: Microbotryum violaceum

characteristic: organism

text: Microbotryum violaceum substrain 11.4.1 mating type chromosome random fragments

characteristic: title


text: University of Oxford

characteristic: INSDC center name

text: live

text: Generic

text: sporidia

text: specific-host=Silene latifolia; country=France; substrain=11.4.7; mating-type=A1

text: Microbotryum violaceum

characteristic: strain

text: Microbotryum violaceum substrain 11.4.7 mating type chromosome random fragments


text: University of Oxford

characteristic: INSDC first public

text: live

text: Generic

text: sporidia

text: specific-host=Silene latifolia; country=France; substrain=6.3.3; mating-type=A2

text: Microbotryum violaceum

text: Microbotryum violaceum substrain 6.3.3 mating type chromosome random fragments


text: University of Oxford

text: live

text: Generic

text: sporidia

text: specific-host=Silene latifolia; country=France; substrain=6.3.6; mating-type=A1

text: Microbotryum violaceum

text: Microbotryum violaceum substrain 6.3.6 mating type chromosome random fragments


text: Institute of Botany (210)

text: live

text: Generic

text: Sporangia

characteristic: dev-stage

text: Plasmopara halstedii

text: Plasmopara halstedii strain Jue_B18z

text: Plasmid

text: pJet


text: Institute of Botany (210)

characteristic: INSDC last update

text: live

text: Generic

text: Sporangia

text: Plasmopara angustiterminalis

text: Plasmopara angustiterminalis strain X03-A1

text: Plasmid

text: pJet


text: Victorian AgriBiosciences Centre, Department of Primary Industries Victoria

text: live

characteristic: INSDC status

text: Generic

text: North African 6 x Aurora 6 F1 plant

characteristic: cultivar

text: Genomic DNA fragments were amplified using 3 types of primers designed to candidate disease resistence genes. 1: Degenerate PCR primers from the literature; 2: Primers designed to proprietary perennial ryegrass EST sequences; 3: Primers designed to candidate gene sequences from perennial ryegrass, oat, wheat and barley downloaded from Genbank. PCR products were cloned into TOPO-TA vectorand sequenced using T3 and T7 primers

text: mature plant

text: E.coli OneShot TOP10 (Invitrogen)

characteristic: lab-host

text: Lolium perenne

text: leaf

text: Genomic derived PCR fragments from putative disease resistance genes

text: TOPO-TA


text: Shanghai University

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: leaf cells

text: This BAC library contains about 230,400 clones with an average insert size of 113 kb, and is equivalent to 16.3-fold coverage of Coix genome.

text: 10-week old seedlings

text: Coix lacryma-jobi

text: HindIII

text: HindIII

text: leaf

text: Coix BAC Library

text: BAC


characteristic: External Id

text: International Crops Research Institute for the Semi-arid Tropics (ICRISAT)

text: live

text: Generic

text: Microsatellite enriched genomic library was constructed from groundnut genotype TMV2 following a modified protocol of Fischer and Bachmmann (1998). The microsatellite enriched fragments were cloned into pJV1 vector and subsequently sequenced using M13 Forward 24-mer Sequencing primer following dideoxynucleotide chain termination method. Microsatellite mining was done using Tandem Repeat Finder software and for the flanking regions primers were designed using Primer3.

text: E. coli DH5 alpha

text: Arachis hypogaea

text: unfurled leaves

text: Microsatellite enriched genomic DNA library of groundnut

text: cloning vector

text: pJV1 (modified pUC19)


text: live

text: Generic

text: ICC 4958

text: Microsatellite enriched genomic library was constructed from chickpea genotype ICC 4958. The microsatellite enriched fragments were cloned into pGEM 3Z(f) vector and subsequently sequenced by dideoxynucleotide chain termination method using T7 forward, SP6 reverse and M13 primers. Microsatellite mining was done using MISA and for the flanking regions primers were designed using Primer3.

characteristic: description

text: Vegetative

text: E.coli DH10B

text: Cicer arietinum

text: MboI

text: TaqI

text: leaf

text: Microsatellite enriched genomic DNA library of chickpea

text: plasmid

text: pGEM 3Z(f)


text: live

text: Generic

text: ICGV 86031

text: Microsatellite enriched genomic library was constructed from groundnut genotype ICGV 86031. The microsatellite enriched fragments were cloned into pCR2.1-TOPO vector and subsequently sequenced by dideoxynucleotide chain termination method using M13 forward and reverse primers. Microsatellite mining was done using MISA and for the flanking regions primers were designed using Primer3.

text: E. coli DH5 alpha

text: Arachis hypogaea

text: RsaI

text: XmnI

text: unfurled leaves

characteristic: tissue-type

text: Microsatellite enriched genomic DNA library of groundnut cv. ICGV 86031


text: Korea Institute of Oriental Medicines

text: live

text: Generic

text: XL1-Blue MRF'

text: Cynanchum auriculatum

text: RAPD fragment from Cynanchum auriculatum

text: pGEM-Teasy

characteristic: vector


text: Korea Institute of Oriental Medicines

text: live

text: Generic

text: XL1-Blue MRF'

text: Cynanchum wilfordii

text: RAPD fragment from Cynanchum wilfordii

text: pGEM-Teasy


text: Korea Institute of Oriental Medicines

text: live

text: Generic

text: XL1-Blue MRF'

text: Fallopia multiflora

text: RAPD fragment from Polygonum multiflorum

text: pGEM-Teasy


text: United States Department of Agriculture (USDA), Agricultural Research Service (ARS)

text: live

text: Generic

text: A partial genomic library constructed from PCR products that contain the O. nubialis Lep1 short interspersed nuclear element (SINE) and flanking downstream genome regions that represent putatively transduced sequence that resemble Helitron-like termini.

text: adult

text: Ostrinia nubilalis

text: male and female

characteristic: sex

text: bivoltine Z-pheromone strain

text: thoracic

text: ARS-CICGRU OnLep-1

text: plasmid

text: pGEM-T


text: East China University of Science and Technology

text: live

text: Generic

text: Real-time polymerase chain reaction for the detection of donkey and horse

text: Equus asinus

characteristic: organism

text: Real-time highly repetitive cot library

text: Plasmid

text: pGEM-T


text: CIRAD

text: live

text: Generic

text: From various cultivars. Flanking regions of newly transposed copies of the tissue culture-induced retrotransposon of rice Tos17 were amplified through adapter-ligation PCR from the right LTR of the element. The LTR sequence was removed from the released sequence.

text: Oryza sativa

text: Oryza sativa FST of the ty1 copia retrotransposon Tos17

characteristic: title


text: CIRAD

characteristic: INSDC center name

text: live

text: Generic

text: Flanking regions of newly transposed copies of the tissue culture-induced retrotransposon of rice Tos17 were amplified through adapter-ligation PCR from the right LTR of the element. The LTR sequence was removed from the released sequence.

text: Oryza longistaminata

text: Oryza longistaminata FST of the ty1 copia retrotransposon Tos17


text: CIRAD

text: live

text: Generic

text: Oryza rufipogon

text: Oryza rufipogon FST of the ty1 copia retrotransposon Tos17


text: University of Illinois

text: live

text: Generic

text: environmental_sample; isolation_source=rumen sample #256; Library of genomic DNA fragments generated by PCR-based suppressive subtractive hybridization of two environmental metagenomes

text: uncultured organism

text: Rumen metagenome subtractive hybridization library


text: San Diego State University

text: live

text: Generic

text: environmental_sample ; isolation_source: healthy human blood

text: uncultured virus

text: uncultured virus DNA from healthy human blood


text: Raritan Valley Community College

text: live

text: Generic

text: environmental_sample. country: USA: New Jersey. isolation_source: soil.

text: uncultured organism

text: Soil Sample from Central New Jersey


text: Hitachi, Ltd.

text: live

text: Generic

text: environmental_sample; isolation_source: wastewater sludge

text: uncultured anammox organism

text: uncultured anammox organism from wastewater sludge


text: University of Parma

text: live

text: Generic

text: environmental_sample ; derived from AFLPs of Olive (Olea europaea, cultivar Tanche) monovarietal oil

text: uncultured fungus

text: uncultured fungus DNA (Pafundo S)


text: Rutgers University

text: live

text: Generic

text: environmental_sample ; isolation_source: buried ice sample ; country: Antarctica: Mullins Valley, Transantarctic Mountains. A bacterial artificial chromosome (BAC) library was generated for community DNA encased in buried ice sample DLE-98-12 from the Mullins Valley, Transantarctic Mountains, Antarctica. Synthetic aperture-radar interferometry, 3H and 21Ne cosmogenic dating of surface boulders, and laser-fusion 40Ar/39Ar radiometric dating of surface ash-fall deposits indicate that ice within Mullins Valley ranges from modern at the valley head to ~300 Ka near the tributary mouth. DLE-98-12 lies half-way down Mullins Valley and is assigned an age of ~100 Ka. Following decontamination and melting procedures of block ice, community DNA was extracted from meltwater and entrapped sediment using the SoilMaster kit (Epicentre Biotechnologies, Madison, WI) according to manufacturer's instructions. BamH1-digested community DNA was ligated into a copy-control pCC1BAC vector (Epicentre). Clones were grown on Lauria Broth under chloramphenicol selection. Colonies on agar plates, frozen ligation reactions, and frozen glycerol stocks of transformants were hand-delivered on dry ice to the Microbial Genomics group at The Institute for Genomic Research (TIGR) for sequencing.

text: ice metagenome

text: Plasmid

text: pCC1BAC (Epicentre Biotechnologies, Madison, WI)


text: Rutgers University

text: live

text: Generic

text: environmental_sample ; isolation_source: buried ice sample ; country: Antarctica: Beacon Valley, Transantarctic Mountains. An end-repaired plasmid library was generated for community DNA encased in buried ice sample EME-98-03 from the upper Beacon Valley, Transantarctic Mountains, Antarctica. Synthetic aperture-radar interferometry, 3H and 21Ne cosmogenic dating of surface boulders, and laser-fusion 40Ar/39Ar radiometric dating of surface ash-fall deposits indicate that ice in the upper and central Beacon Valley could be as much as 10 Ma. EME-98-03 lies in the zone estimated to be up to 8 Ma, making it the oldest known ice on Earth. Following decontamination and melting procedures of block ice, community DNA was extracted from meltwater and entrapped sediment using the SoilMaster kit (Epicentre Biotechnologies, Madison, WI) according to manufacturer's instructions. End-repaired community DNA was ligated into the blunt cloning-ready Copy Control pCC1 Vector (Epicentre). Clones were grown on Lauria Broth under chloramphenicol selection. Colonies on agar plates, frozen ligation reactions, and frozen glycerol stocks of transformants were hand-delivered on dry ice to the Microbial Genomics group at The Institute for Genomic Research (TIGR) for sequencing.

text: ice metagenome

text: Plasmid

text: pCC1 Blunt Cloning-Ready(Epicentre Biotechnologies, Madison, WI)


text: National Food Institute DTU; DOE Joint Genome Institute

text: live

text: Generic

text: isolation_source: fecal sample from 1 month old human infant; environmental_sample; collection_date: 14-Jun-2004

text: Homo sapiens

text: uncultured bacterium


text: DOE Joint Genome Institute

text: live

text: Generic

text: isolation_source: fecal sample from 11 month old human infant; environmental_sample; collection_date: 19-Apr-2005

text: Homo sapiens

text: uncultured bacterium


text: live

text: Generic

text: isolation_source: fecal sample from adult human 1 month after childbirth; environmental_sample; collection_date: 14-Jun-2004

text: Homo sapiens

text: uncultured bacterium


text: DOE Joint Genome Institute

text: live

text: Generic

text: isolation_source: fecal sample from adult human 11 months after childbirth; environmental_sample; collection_date: 18-Apr-2005

text: Homo sapiens

text: uncultured bacterium


text: University of Delaware

text: live

text: Generic

text: RAPD-PCR amplicons of virioplankton concentrates collected from Chesapeake Bay. environmental_sample; country: USA: Chesapeake Bay.

text: uncultured virus

text: Chesapeake Bay RAPD-PCR amplicons

text: Plasmid

text: pCRII-TOPO or pCR4-TOPO TA cloning vectors (Invitrogen, Carlsbad, CA, USA)


text: Michigan State University

text: live

text: Generic

text: The library was constructed using the Epicenter CopyControl Fosmid Library production kit, (http://epibio.com/pdftechlit/171pl128.pdf).

text: DM1-3 516R44 (CIP801092)

characteristic: isolate

text: E. coli EPI300

text: Solanum phureja

text: Eco72 I

text: Eco72 I

text: Fosmid


text: The Interuniversity Institute for Marine Sciences

characteristic: INSDC first public

text: live

text: Generic

text: environmental DNA sample. Genomic DNA obtained from 20 l seawater sample (size fraction 0.45 to 20 m) was cloned into fosmid vector pCC1FOS using copy control fosmid library production kit.

text: Gulf of Aqaba, station A, 20 m, March 2003

text: E.coli EPI300-T1

text: uncultured crenarchaeote

text: 3320 fosmid clone library uncultured crenarchaeote

text: fosmid


text: live

text: Generic

text: E.coli EPI300-T1

text: uncultured euryarchaeote

text: 3320 fosmid clone library uncultured euryarchaeote

text: fosmid