text: Kazusa DNA Research Institute

characteristic: INSDC first public

text: live

text: Generic

text: sporophytes

text: Neopyropia yezoensis

text: Porphyra yezoensis TU-1 sporophytes


status: PUBLIC

characteristic: External Id

text: Kazusa DNA Research Institute

characteristic: INSDC center name

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Mus musculus

characteristic: organism

text: male

tag: attribute

characteristic: sex

text: BALB/c

characteristic: strain

text: brain

characteristic: tissue-type

text: Mus musculus male brain BALB/c

characteristic: title


text: Kazusa DNA Research Institute

text: live

text: Generic

text: Mus musculus

text: male

text: brain

text: Mus musculus male brain C57BL/6


text: Horticulture College of Northwest Sci-Tech University of Agriculture and Forestry

text: live

text: Generic

characteristic: cultivar

text: Vptua1 is a differential display cDNA of Baihe-35-1 leaves which were infected with the fungus Uncinula necator in August,2002, in Yangling Shannxi, China. These ESTs was screening by DDRT-PCR. The expression of them was high in the infected leaves while not found in the healthy leaves. Plants were symptomatic at the time of collection.

characteristic: description

text: Vitis pseudoreticulata

text: female

text: leaves

text: Baihe-35-1 leaves


text: live

text: Generic

text: mRNA differential display was employed to study Powdery Mildew resistant gene express differential in the wild grape(vitis L.) native to China. cDNA fragments relate to Powdery Mildew resistant gene were obtained, these results will be helpful to the cloning of disease resistance gene .

text: Vitis pseudoreticulata

text: female

text: leaves

text: Baihe-35-1 leaves differential display


text: Kyoto University

text: live

text: Generic

characteristic: cell-line

text: Bright Yellow-2

text: suspension cultured cells acclimated to low level boron

text: 5-day-old cell

characteristic: dev-stage

text: Nicotiana tabacum

text: Nicotiana tabacum BY-2


text: Shizuoka University

text: live

text: Generic

text: Miyagawa-wase

text: anthesis stage

text: Citrus unshiu

text: whole ovary

text: Citrus unshiu whole ovary anthesis stage


text: Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais

text: live

text: Generic

text: sequences obtained by differential display approach

text: adult

text: Canis familiaris

characteristic: lab-host

text: Dipylidium caninum

text: Dipylidium caninum differential display


characteristic: INSDC first public

text: live

text: Generic

text: adult

text: Canis familiaris

text: Ancylostoma caninum

text: Male

text: Ancylostoma caninum male adult differential display


characteristic: External Id

text: live

text: Generic

text: adult

text: Canis familiaris

text: Ancylostoma caninum

text: Female

text: Ancylostoma caninum female adult differential display


text: Ghent University Hospital

characteristic: INSDC center name

text: live

text: Generic

text: Neuroblastoma

characteristic: cell type

text: PCR select - suppression subtractive hybridisation (SSH)

text: Esherichia coli

text: Homo sapiens

text: Plasmid

text: pGEM-T Easy

characteristic: vector


text: E. I. DuPont de Nemours and Company

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Eucalyptus grandis apical leaves (first two pairs of branch tip)

text: Eucalyptus grandis

text: EcoRI

text: XhoI

text: leaf

text: pBluescript SK+


text: E. I. DuPont de Nemours and Company

text: live

text: Generic

characteristic: NCBI submission package

text: Eucalyptus tereticornis capsules (older flowers, lost stamens, possibly fertilised) from adult tree

text: adult

text: Eucalyptus tereticornis

characteristic: organism

text: EcoRI

text: XhoI

text: carpels

text: pBluescript SK+


text: E. I. DuPont de Nemours and Company

text: live

text: Generic

text: Eucalyptus tereticornis flower buds from adult tree

text: adult

text: Eucalyptus tereticornis

text: EcoRI

text: XhoI

text: flower

characteristic: tissue-type

text: pBluescript SK+


text: E. I. DuPont de Nemours and Company

text: live

text: Generic

text: Eucalyptus grandis juvenile leaves

text: juvenile

text: Eucalyptus grandis

text: EcoRI

text: XhoI

text: leaf

characteristic: title

text: pBluescript SK+


text: E. I. DuPont de Nemours and Company

text: live

text: Generic

text: Eucalyptus grandis leaves/petioles/stems

text: Eucalyptus grandis

text: EcoRI

text: XhoI

text: leaf

text: pBluescript SK+


text: E. I. DuPont de Nemours and Company

text: live

text: Generic

text: Eucalyptus grandis roots from seedlings 6-9 months

text: seedlings 6-9 months

text: Eucalyptus grandis

text: EcoRI

text: XhoI

text: root

text: pBluescript SK+


text: University of British Columbia

text: live

text: Generic

text: differential display (RAP-PCR) product from adult liver

text: liver

characteristic: organ

text: Fundulus heteroclitus

text: New Hampshire

characteristic: strain

text: Fundulus heteroclitus liver RAP-PCR


text: National Eye Institute

text: live

text: Generic

text: A human iris library (bx) was normalized by self-subtraction. One portion of double stranded plasmid DNA representing the library was linearized by NotI. This NotI digested library was used as a template for biotinylated RNA synthesis using SP6 RNA polymerase. Another portion of the double stranded plasmid library was converted to single-stranded circles in vitro using Gene II and Exonuclease III (Life Technologies). Single-stranded DNA (1 mg) was hybridized (C0t 500) with 41 mg of Bio-RNA and vector blocking oligonucleotides. The hybridized Bio-RNA/ss-circles were removed by streptavidin:phenol extraction. EST analysis was performed on the library at the NIH Intramural Sequencing Center(NISC).

characteristic: description

text: Adult

text: Eye

text: Homo sapiens

text: Iris

text: Human Iris cDNA (Normalized): fg

text: Plasmid


text: National Eye Institute

text: live

text: Generic

text: A human lens library (by) was normalized by self-subtraction. One portion of double stranded plasmid DNA representing the library was linearized by NotI. This NotI digested library was used as a template for biotinylated RNA synthesis using SP6 RNA polymerase. Another portion of the double stranded plasmid library was converted to single-stranded circles in vitro using Gene II and Exonuclease III (Life Technologies). Single-stranded DNA (1 mg) was hybridized (C0t 500) with 41 mg of Bio-RNA and vector blocking oligonucleotides. The hybridized Bio-RNA/ss-circles were removed by streptavidin:phenol extraction. EST analysis was performed on the library at the NIH Intramural Sequencing Center(NISC).

text: Adult

characteristic: dev-stage

text: Eye

text: Homo sapiens

text: Lens

text: Human Lens cDNA (Normalized): fs

text: Plasmid


text: National Eye Institute

text: live

text: Generic

text: The cDNA, directionally cloned in the pSPORT1 vector (Life Technologies, Rockville, MD), was constructed at Bioserve Biotechnology (Laurel, MD) essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). Sublibraries (designated ho, hp, and hq) were made from the first three 35-uL fractions of cDNA from a Sephacryl S-500 HR resin column. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center(NISC).

text: Adult

characteristic: lab-host

text: Eye

text: Homo sapiens

text: Trabecular meshwork

text: Human Trabecular meshwork cDNA: hohphq

text: Plasmid


text: Washington University School of Medicine

characteristic: INSDC first public

text: live

text: Generic

text: This library was created from laser-captured isthmal cells from tox176 transgenic mice. 1st strand of cDNA was synthesized with reverse transcriptase and oligo(dT) beads, then cDNA was amplified by PCR using modified SMART primers. The final cDNA was cloned in pAMP1 vector in annealing reaction with Uracil DNA Glycosylase (UDG). Library constructed by Y.Korshunova and M. Lovett. Library materials provided by Mills JC & Gordon JI.

text: adult

text: Mus musculus

text: Gastric Epithelial Progenitor

text: Gastric Epithelial Progenitor

text: plasmid


characteristic: External Id

text: live

text: Generic

text: The library was constructed by Claire Murphy and Dr. Makedonka Dautova at Washington University, St. Louis. Oligo(dT)-SL1 PCR based library. Meloidogyne chitwoodi egg cDNA PCR products of size >400 nucleotides containing SL1 on the 5' end and oligo(dT) on the 3' end were non-directionally cloned into pCRII-TOPO(Invitrogen) following the Topo TA cloning protocol. Eggs were provided by Dr. David Bird of North Carolina State University, Raleigh, NC (david_bird@ncsu.edu).

text: eggs

text: Meloidogyne chitwoodi

text: EcoRI

text: EcoRI

text: Meloidogyne chitwoodi egg SL1 TOPO v1

text: plasmid (kanamycin and ampicillin resistant)

text: pCRII-TOPO (Invitrogen)


text: Sun Yat-sen University

characteristic: INSDC center name

text: live

text: Generic

text: ESTs generated from a normal nasopharynx cDNA library from southern Chinese

text: Homo sapiens

text: normal nasopharynx

text: human nasopharynx


text: University of Otago

text: live

text: Generic

text: Panagrolaimus davidi

text: Panagrolaimus davidi mRNA


text: CSIC-IATA

text: live

text: Generic

text: Fortune

characteristic: cultivar

text: Citrus clementina x Citrus reticulata

text: flavedo

text: Citrus clementina x Citrus reticulata flavedo


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: Cloned unidirectionally from mRNA prepared from 800 blastocysts. Primer: NotI(dT): 5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNAs were cloned into the NotI/SalI sites of a pSPORT vector (Life Technologies). Two different size selections: B1 (larger inserts) and B3. Average insert size of combined library is 1 kb.

text: embryo (pre-implantation)

text: Mus musculus

text: Knowles/Solter blastocyst


text: live

text: Generic

text: 1st strand cDNA was primed with a Not I - oligo (dT) primer [5' TGTTACGAATCTGAAGTGGGAGCGGCCGCCCTTTTTTTTTTTTT TTTTTTTTTTTT 3']; double-stranded cDNA was ligated to EcoRI adaptors [AATTCGGATCCTTG], digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library constructed by R. Barstead (Oklahoma Medical Research Foundation). The C2C12 cell line (available from ATCC, catalog # CRL-1772) differentiates rapidly, forming contractile myotubes and producing characteristic muscle proteins.

text: Mus musculus

text: Barstead stromal cell line MPL-RB5


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size: 1.0 kb; Uni-ZAP XR Vector; 5' adaptor sequence: 5' GAATTCGGCACGAG 3' 3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3' Library provided by David Beier and Lisa Guay-Woodford.

text: juvenile (7 days old)

text: SOLR (kanamycin resistant)

text: Mus musculus

text: Beier day 7 kidney


text: live

text: Generic

text: Cloned unidirectionally from mRNA prepared from 5000 unfertilized eggs. Primer: SalI(dT): 5'-CGGTCGACCGTCGACCGTTTTTTTTTTTTTTT-3'. cDNAs were cloned into the MluI/SalI sites of a modified pBluescribe vector using commercial linkers (NEB). Average insert size: 1.0 kb.

text: Mus musculus

text: Knowles/Solter egg


text: National Eye Institute

text: live

text: Generic

text: RNA was extracted from 2 human lacrimal glands. A directionally cloned cDNA library in the pCMVSPORT6 vector(Life Technologies) was constructed at Bioserve Biotechnology (Laurel MD) essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC).

text: Adult

text: Eye

text: Homo sapiens

text: lacrimal gland

text: Human lacrimal gland, unamplified: oj

text: Plasmid

characteristic: vector


text: International Centre for Genetic Engineering and Biotechnology (ICGEB)

characteristic: INSDC last update

text: live

text: Generic

text: Two-week-old seedlings

text: Leaf

text: Cenchrus americanus

text: EcoRI

text: XhoI

text: Entire leaf tissue

text: Pennisetum glaucum seedlings exposed to cold (4 C) stress

text: Phage

text: Lambda Zap


text: live

characteristic: INSDC status

text: Generic

text: Two-week-old seedlings

text: Root

text: Cenchrus americanus

text: EcoRI

text: XhoI

text: Entire root tissue

text: Pennisetum glaucum seedlings root tissue

text: Phage

text: Lambda Zap


text: live

text: Generic

characteristic: NCBI submission model

text: Two-week-old seedlings

text: Leaf

text: Cenchrus americanus

text: EcoRI

text: XhoI

text: Entire leaf tissue

text: Pennisetum glaucum seedlings exposed to salt (500 mM NaCl)

text: Phage

text: Lambda Zap


text: live

text: Generic

characteristic: NCBI submission package

text: Two-week-old seedlings

text: Leaf

text: Cenchrus americanus

text: EcoRI

text: XhoI

text: Entire leaf tissue

text: Pennisetum glaucum seedlings exposed to drought stress

text: Phage

text: Lambda Zap


text: University of New Hampshire

text: live

text: Generic

text: Total RNA was isolated from 20 day post-anthesis testa tissue and used in a subtraction hybridization procedure as according to the Clontech PCR-Select cDNA Subtraction kit (PT1117-1) (Clontech, Palo Alto, CA).

text: 20 days post-anthesis

text: Testa

text: Cucurbita pepo

characteristic: organism

text: Cucurbita pepo testa subtracted cDNA


text: Animal and Natural Resources Institute, USDA

text: live

text: Generic

text: Lymphocyte

characteristic: cell type

text: Normalized library from chicken gut infected with coccidia duodenum and middle gut.

text: Adult

text: Intestine

text: Gallus gallus

text: SalI

text: NotI

text: mixed

characteristic: sex

text: white leghorn SC

text: Gut

characteristic: tissue-type

text: 1GAL - Chicken Intestinal Lymphocyte

text: plasmid


text: University of Notre Dame

text: live

text: Generic

text: Plasmodium berghei-infected rat blood-fed adult female An. gambiae mosquitoes were flash frozen after a 30 hour incubation of adult mosquitoes at 19 degrees Celsius. Total RNA extracted from abdomens separated from remaining carcass. CDNA inserts >500 bp cloned directionally into lTriplEx2; Sfi IA site is 5'. Non-normalized and Non-amplified phagemid library. Single pass sequencing reactions from 5' end.

text: Female adult 5-7 days post eclosion

text: E. coli XLI-Blue

text: Anopheles gambiae

text: Sfi IA

text: Sfi IB

text: female

characteristic: strain

text: Abdomens

text: Infected Rat Blood-fed (IRB) An.gam. 30 hr Abdomen Library

characteristic: title

text: Phagemid

text: lamdaTriplEx2 (Clontech)


text: University of Notre Dame

text: live

text: Generic

text: Naive Rat Blood-fed adult female An. gambiae mosquitoes were flash frozen after a 30 hour incubation of adult mosquitoes at 19 degrees Celsius. Total RNA extracted from abdomens separated from remaining carcass. CDNA inserts >500 bp cloned directionally into lTriplEx2; Sfi IA site is 5'. Non-normalized and Non-amplified phagemid library. Single pass sequencing reactions from 5' end.

characteristic: description

text: Female adult 5-7 days post eclosion

characteristic: dev-stage

text: E. coli XLI-Blue

text: Anopheles gambiae

text: Sfi IA

text: Sfi IB

text: female

text: Abdomens

text: Naive Rat Blood-fed (RB) An.gam. 30 hr Abdomen Library

text: Phagemid

text: lambdaTriplEx2 (Clontech)


text: University of Notre Dame

characteristic: INSDC first public

text: live

text: Generic

text: Sugar-fed adult female An. gambiae mosquitoes were flash frozen after a 30 hour incubation of adult mosquitoes at 19 degrees Celsius. Total RNA extracted from abdomens separated from remaining carcass. CDNA inserts >500 bp cloned directionally into lTriplEx2; Sfi IA site is 5'. Non-normalized and Non-amplified phagemid library. Single pass sequencing reactions from 5' end.

text: Female adult 5-7 days post eclosion

text: E. coli XLI-Blue

characteristic: lab-host

text: Anopheles gambiae

text: Sfi IA

text: Sfi IB

text: female

text: Abdomens

text: Sugar-fed (S) An.gam. 30 hr Abdomen Library

text: Phagemid

text: lambdaTriplEx2 (Clontech)


characteristic: External Id

text: Washington University School of Medicine

characteristic: INSDC center name

text: live

text: Generic

text: The library was constructed by Claire Murphy and Dr. Makedonka Dautova at Washington University, St. Louis. Oligo(dT)-SL1 PCR based library. Meloidogyne incognita egg cDNA PCR products of size >400 nucleotides containing SL1 on the 5' end and oligo(dT) on the 3' end were non-directionally cloned into pCRII-TOPO(Invitrogen) following the Topo TA cloning protocol. Roots infected with Meloidogyne incognita were provided by Dr. Barry Shortt at Divergence, Inc. and adult females were extracted by Dr. Makedonka Dautova.

text: adult females

text: Meloidogyne incognita

text: EcoRI

text: EcoRI

text: Meloidogyne incognita parasitic adult female SL1 TOPO v1

text: plasmid (kanamycin and ampicillin resistant)

text: pCRII-TOPO (Invitrogen)


text: live

text: Generic

text: The library was constructed by Claire Murphy and Dr. Makedonka Dautova at Washington University, St. Louis (mdautova@watson.wustl.edu). The cDNA was made by using Dynabead oligo-dT priming (Dynal). PCR based library using a modified protocol from the SMART PCR cDNA Synthesis Kit from Clontech. Directionally cloned into the UDG sites of pAMP1. Heterodera schachtii J2 were provided by Bartel Vanholme of Ghent University (Bartel.Vanholme@rug.ac.be).

text: Heterodera schachtii

text: Not I

text: Sal I

text: Heterodera schachtii J2 pAMP1 v1

text: plasmid- ampicillin resistance

characteristic: vector


characteristic: INSDC last update

text: live

text: Generic

text: The library was constructed by Claire Murphy and Dr. Makedonka Dautova at Washington University, St. Louis. Oligo(dT)-SL1 PCR based library. Meloidogyne arenaria egg cDNA PCR products of size >400 nucleotides containing SL1 on the 5' end and oligo(dT) on the 3' end were non-directionally cloned into pCRII-TOPO(Invitrogen) following the Topo TA cloning protocol. Eggs were provided by Dr. David Bird of North Carolina State University, Raleigh, NC (david_bird@ncsu.edu).

text: eggs

text: Meloidogyne arenaria

text: EcoRI

text: EcoRI

text: Meloidogyne arenaria egg SL1 TOPO v1

text: pCRII-TOPO (Invitrogen)


text: Cereal Research Centre, Agriculture and Agri-food Canada

text: live

characteristic: INSDC status

text: Generic

text: AC McDuff

characteristic: cultivar

text: mRNA obtained from bolls 12 days after flowering

text: 12 days after flowering

text: Linum usitatissimum

text: NotI

text: MluI

text: Bolls

text: Lin

text: pBluescript KS+ (Stratagene)


text: University of New Mexico

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: BS90 (Salvador) and M-line strains of B. glabrata were exposed to S. mansoni for 12 hours. mRNA was harvested from each strain and cDNA was generated using oligo-dT primers. The BS90 cDNA was subtracted against the M-line cDNA using the PCR-Select Suppressive Subtraction Hybridization kit (SSH) (Clontech, Palo Alto, CA). The resulting library was cloned into pCR2.1 (Invitrogen, Carlsbad, CA) and sequenced using plasmid-specific primers (M13 rev and/or M13 -20).

text: whole body

characteristic: organ

text: Biomphalaria glabrata

text: EcoRI

text: EcoRI

text: hermaphrodite

text: B. glabrata BS90 - M line (exposed) SSH library

text: plasmid


text: ESALQ - University of Sao Paulo

text: live

text: Generic

text: This cDNA library was constructed with the SuperScript Plasmid System with Gateway Tecnology kit (Invitrogen), following manufacture's protocols. Plasmid DNA was purified using a modified alkaline lysis method. Sequencing reactions were conducted using the DYEnamic ET Dye Terminator sequencing kit (Amersham Biosciences) according to the manufacturer's recommendations. Clones were sequenced by the 5' end with T7 primer.Sequencing reactions were analyzed on MegaBACE 1000 automated DNA sequencer (Amersham Biosciences). The quality and clustering of the ESTs were analyzed using the softwares Phred/Cap3/Consed. Only EST sequences with Phred quality greater than 20 and at least 200 bp were considered for clustering.

text: 50 hours old - embryonic stage

text: DH5 alpha

text: Gallus gallus

characteristic: organism

text: NotI

text: SalI

text: Somites associated with the neural tube

text: Somite associated with neural tube - SM1

text: Plasmid


text: ESALQ - University of Sao Paulo

text: live

text: Generic

text: 6 days old - embryonic stage

text: DH5 alpha

text: Gallus gallus

text: NotI

text: SalI

text: Whole embryos

characteristic: tissue-type

text: Whole embryo - EM1

text: Plasmid


text: ESALQ - University of Sao Paulo

text: live

text: Generic

text: pool of 3 1/2 days old, 4 1/2 days old and 6 days old - embryonic stage

text: DH5 alpha

text: Gallus gallus

text: NotI

text: SalI

text: Limb buds

text: Limb Bud - LB1

text: Plasmid


text: USDA-ARS, Washington State University

text: live

text: Generic

text: whole leaf

characteristic: cell type

text: cDNA-AFLP fragments differentially expressed between the ascochyta blight resistant, FLIP84-92C(2), and the susceptible germplasm line, PI 359075(1)

text: two weeks after germination

text: Cicer arietinum

text: FLIP84-92C(2); PI 359075(1)

characteristic: strain

text: leaf

text: chickpea leaf cDNA-AFLP fragments


text: INRA

text: live

text: Generic

text: microspores

text: four days after in vitro culture

text: Zea mays

text: Zea mays cv. DH229 cDNA-AFLP sample

characteristic: title


text: National Cancer Institute / NIH

text: live

text: Generic

text: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT (30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.50 kb (range 0.9-3.5 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA).

characteristic: description

text: DH10B (T1 phage-resistant)

text: Testis

text: Macaca mulatta

text: SfiI (ggccattatggcc)

text: SfiI (ggccgcctcggcc)

text: Testis

text: plasmid (chloramphenicol resistant, grown at 30C)

text: pDNR-LIB


text: National Cancer Institute / NIH

characteristic: INSDC first public

text: live

text: Generic

text: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT (30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.67 kb (range 0.8-3.5 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA).

text: DH10B (T1 phage-resistant)

text: Testis

text: Macaca mulatta

text: SfiI (ggccattatggcc)

text: SfiI (ggccgcctcggcc)

text: Testis

text: pDNR-LIB


text: Chulalongkorn University

text: live

text: Generic

text: Adult

characteristic: dev-stage

characteristic: lab-host

text: Penaeus monodon

text: hemocytes

text: Shrimp Lambda Zip Lox Library

text: Phage

text: Lambda Zip Lox


characteristic: External Id

text: University of Georgia

characteristic: INSDC center name

text: live

text: Generic

text: Gossypium arboreum

text: EcoRI

text: XhoI

text: Leaves

text: Coau

text: Phagemid

text: lambdaZAP Express


text: University of Georgia

characteristic: INSDC last update

text: live

text: Generic

text: Gossypium arboreum

text: EcoRI

text: XhoI

text: Leaves

text: Gate

text: Phagemid

text: lambdaZAP Express

characteristic: vector


text: University of Georgia

text: live

characteristic: INSDC status

text: Generic

characteristic: cultivar

text: Gossypium arboreum

text: EcoRI

text: XhoI

text: Leaves

text: Gate-Unig

text: Phagemid

text: lambdaZAP Express


text: Institute of Plant Genetics and Crop Plant Research (IPK)

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Sloop

text: Due to the cloning system used blue/white selection for recombinants is not 100% reliable. Average insert size is 1.3 kb.

text: coleoptile, 3 days old

text: Hordeum vulgare

text: SalI (5-end of cDNA)

text: NotI (3-end of cDNA)

text: coleoptile

text: CH

text: pSPORT


text: Hainan University, Biotechnology Institute

text: live

text: Generic

text: Kandelia candel

characteristic: organism

text: Kandelia candel cDNA


text: IGBMC

text: live

text: Generic

text: Cells from transgenic embryos expressing GFP under control of regulatory elements of the shh gene. Directly subcloned fragments resulting from the nested PCR of the Clontech PCRSelect subtraction procedure, enriched for genes expressed in the midline

text: shield stage, 6 hrs post-fertilisation

text: TOP10 OneShot cells

text: Danio rerio

text: Zebrafish shield stage midline cells

text: pCRII-TOPO


text: University of Wisconsin-Milwaukee

text: live

text: Generic

text: RZPD609 clones enriched for genes expressed in the midline

text: 24 hour post fertilisation and adult

text: E. coli XL1-Blue MRF'

text: Danio rerio

text: whole embryo

characteristic: tissue-type

text: Zebrafish 24h stage embryos and adult liver, RZPD609


text: Universite de Bordeaux I, Institut National de la Recherche Agronomique

text: live

text: Generic

text: Cabernet Sauvignon

text: Oriented library

text: Ripe stage

text: Fruit skin

characteristic: organ

text: Vitis vinifera

text: SfiIA

text: SfiIB

text: Ripe Grape Berry Skins Lambda Triplex2 Library

text: Phage

text: Lambda Triplex2


text: live

text: Generic

text: Cabernet Sauvignon

text: Oriented library

text: green stage

text: Fruit skin

text: Vitis vinifera

text: SfiIA

text: SfiIB

text: Green Grape Berry Skins Lambda Triplex2 Library

text: Phage

text: Lambda Triplex2


text: live

text: Generic

text: The library was constructed according Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. Denatured RNA was size fractionated on a 1% agarose gel.First strand cDNA synthesis was primed with oligo-dT primer containing a Not I site. Double strand cDNA was size selected according to mRNA size fraction,ligated with EcoR I adaptor, digested with NotI and then cloned directionally into pYX-Asc vector. The library tag sequence located between the Not I site and the polyA tail is CGAACTGAAT. This library was created for the University Iowa Brain Anatomy Project (BMAP): 'Gene Discovery in the Developing Mouse Nervous System', supported by National Institute of Mental Health (NIMH), Hemin Chin, Ph.D., program coordinator.

text: 1,5, and 15 days newborn

text: DH10B (T1 phage resistant)

text: Brain

text: Mus musculus

text: EcoR I

text: Not I

characteristic: strain

text: whole brain

text: Plasmid

text: pYX- Asc


text: live

text: Generic

text: The library was constructed according Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. Denatured RNA was size fractionated on a 1% agarose gel.First strand cDNA synthesis was primed with oligo-dT primer containing a Not I site. Double strand cDNA was size selected according to mRNA size fraction, ligated with EcoR I adaptor, digested with NotI and then cloned directionally into pYX-Asc vector. The library tag sequence located between the Not I site and the polyA tail is CTGCGTCCTC. This library was created for the University Iowa Brain Anatomy Project (BMAP): 'Gene Discovery in the Developing Mouse Nervous System', supported by National Institute of Mental Health (NIMH), Hemin Chin, Ph.D., program coordinator.

text: embryo 15.5,16.5,17.5,18.5 dpc

text: DH10B (T1 phage resistant)

text: Eye

text: Mus musculus

text: EcoR I

text: Not I

text: whole eye

characteristic: title

text: Plasmid

text: pYX- Asc


text: Institute of Botany, Chinese Academy of Sciences

text: live

text: Generic

text: Two-months old seedling of Leymus chinensis growing on An Da grassland of northern China is sampled. The conditions of soil are followed: soil organic matter: 3.92%; soil total nitrogen content: 0.216%; soil buffer potassium: 869.3 mg/kg; aqueous sodium ion: 594.10 mg/kg; aqueous magnesium ion: 8.79 m.e./100g soil; aqueous calcium ion: 16.21 m.e./100g soil; pH: 11.2. The time of sampling was early, August (04/08/2002). The young and actively growing state of the source tissue was used to generate the library. The mRNA was selected with PolyATtract mRNA Isolation SystemsIII (Promega). The cDNA was ligated unidirectionally into the EcoRI and XhoI site of the lambda-ZAP vector (Stratagene).

characteristic: description

text: 2 months old plant

text: Leymus chinensis

text: Leaf

text: Leymus chinensis leaf cDNA library

text: lambda-ZAP


characteristic: INSDC first public

text: live

text: Generic

characteristic: cell-line

text: Trichoplusia ni

text: Trichoplusia ni cell line(BT1-TN-5B1)


text: College of Agronomy and Biotechnology, China Agricultural University

text: live

text: Generic

text: Gossypium hirsutum

text: cotton fiber

text: Cotton fiber DDRT


text: Univerity of Heidelberg

text: live

text: Generic

text: Caenorhabditis elegans

text: Caenorhabditis elegans N2 (Moehrlen F)


text: Institut of viticulture

text: live

text: Generic

text: infected vitis leaf

text: Plasmopara viticola

text: zoospores

text: Plasmopara viticola zoospores


text: Center for Developmental Biology (CDB), RIKEN

text: live

text: Generic

text: Hamburger Hamilton stage 14

characteristic: dev-stage

text: Gallus gallus

text: Gallus gallus Hamburger Hamilton stage 14


characteristic: External Id

text: The Institute of Physical and Chemical Research (RIKEN)

text: live

text: Generic

text: pooled tissues:(tissue_type=bladder,sex=mix, cell_line=RCB-0544 MBT-2),(tissue_type=embryo,sex=mix, cell_line=RCB-0549 Cle-H3),(strain=DDD,tissue_type=spleen, sex=mix,cell_line=RCB-0559 K-1.fl),(strain=BALB/C,tissue_type=blood,sex=mix, cell_line=RCB-0035 WEHI-3),(strain=C57BL/6, tissue_type=skin,sex=mix,cell_line=RCB-1283 B16 melanoma),(strain=BALB/C,tissue_type=sarcoma,sex=mix, cell_line=RCB-0464 Meth-A),(strain=ICR,tissue_type=embryo, sex=mix,cell_line=RCB-0545 OHTA),(strain=C3H/J,dev_stage=7 days embryo,sex=mix,cell_type=nullipotent stem cell,cell_line=CRL-2070 NE),(strain=BALB/C,tissue_type=kidney,sex=mix, cell_line=CCL-142 RAG),(strain=Swiss Webster,tissue_type=submandibular gland,sex=male,cell_line=CRL-1734 SCA-9 clone 15),(strain=BALB/CRos,dev_stage=1.5 years,tissue_type=mammary gland,sex=female,cell_line=CRL-2116 JC),(strain=BALB/C,sex=mix,cell_line=CRL-1751 WEHI 164),(strain=C3H,tissue_type=brain,sex=mix, cell_line=CRL-1443 BC3H1),(strain=BALB/C,tissue_type=B lymphocyte,sex=mix,cell_type=B cells,cell_line=CRL-1669 BCL1 Clone 13.20-3B3),(strain=BDF1,dev_stage=5 months,tissue_type=bone marrow,sex=female,cell_type=stroma cell,cell_line= CRL-2028 SR-4987),(strain=C57BL/6,tissue_type=testis, sex=mix,cell_type=Leydig cells,cell_line=CRL-2065 MLTC-1),(strain=(BALB/C X NZB)F1,tissue_type=B lymphocyte,sex=mix,cell_type=B cells,cell_line=CRL-1702 WEHI 231)

text: Mus musculus

text: RIKEN full-length enriched, pooled cell lines, RCB-0544,etc.


text: Northeast Agricultural University

characteristic: INSDC center name

text: live

text: Generic

text: Beta vulgaris

text: Beta vulgaris library (Dai J)


text: First Military Medical University

characteristic: INSDC last update

text: live

text: Generic

text: lymphocytes

characteristic: cell type

text: New Zealand White

text: Oryctolagus cuniculus

text: blood

text: Oryctolagus cuniculus blood lymphocytes


text: Genoplante

text: live

characteristic: INSDC status

text: Generic

text: jet neuf

text: Brassica napus

text: seed


text: Genoplante

text: live

text: Generic

text: samourai (restored line)

characteristic: cultivar

text: Brassica napus

text: anthers


text: Genoplante

text: live

text: Generic

characteristic: NCBI submission model

text: Helianthus annuus

text: leaves


text: Genoplante

text: live

text: Generic

characteristic: NCBI submission package

text: Helianthus annuus

text: terminal bud


text: Genoplante

text: live

text: Generic

text: Helianthus annuus

characteristic: organism

text: stem


text: Genoplante

text: live

text: Generic

text: Helianthus annuus

text: 4 days after self-pollination embryo

characteristic: tissue-type


text: Genoplante

text: live

text: Generic

text: Helianthus annuus

text: embryo


text: Genoplante

text: live

text: Generic

text: Helianthus annuus

text: ovary


text: Genoplante

text: live

text: Generic

text: Helianthus annuus

text: 15 days after self-pollination embryo


text: Genoplante

text: live

text: Generic

text: Helianthus annuus

text: cotyledons


text: Genoplante

text: live

text: Generic

text: recital

text: Triticum aestivum

text: leaf


text: Genoplante

text: live

text: Generic

text: recital

text: Triticum aestivum

text: root


text: Genoplante

text: live

text: Generic

text: recital

text: Triticum aestivum

text: leaf one


text: Genoplante

text: live

text: Generic

text: recital

text: Triticum aestivum

text: grain (118 degrees per day after pollination)


text: Genoplante

text: live

text: Generic

text: recital

text: Triticum aestivum

text: grain (45 degrees per day after pollination)


text: Genoplante

text: live

text: Generic

text: recital

text: Triticum aestivum

text: grain (174 degrees per day after pollination)


text: Genoplante

text: live

text: Generic

text: recital

text: Triticum aestivum

text: grain (356 degrees per day after pollination)


text: Genoplante

text: live

text: Generic

text: recital

text: Triticum aestivum

text: grain (468 degrees per day after pollination)


text: Genoplante

text: live

text: Generic

text: recital

text: Triticum aestivum

text: grain (550 degrees per day after pollination)


text: Genoplante

text: live

text: Generic

text: recital

text: Triticum aestivum

text: grain (608 degrees per day after pollination)


text: Genoplante

text: live

text: Generic

text: recital

text: Triticum aestivum

text: grain (750 degrees per day after pollination)


text: Genoplante

text: live

text: Generic

text: recital

text: Triticum aestivum

text: ovary

text: OV


text: Genoplante

text: live

text: Generic

text: mixture

text: Zea mays


text: Genoplante

text: live

text: Generic

text: Zea mays

text: aerial, root, whole plant

text: MAB


text: Genoplante

text: live

text: Generic

text: Zea mays

text: whole plant

text: MAC


text: Genoplante

text: live

text: Generic

text: Zea mays

text: whole plant

text: MAD


text: Genoplante

text: live

text: Generic

text: Zea mays

text: aerial, root, whole plant

text: MAR


text: Genoplante

text: live

text: Generic

text: Zea mays

text: aerial, root, whole plant

text: QAC


text: Genoplante

text: live

text: Generic

text: Zea mays

text: aerial, root, whole plant

text: QAD


text: Genoplante

text: live

text: Generic

text: Zea mays

text: pericarp

text: QAE


text: Genoplante

text: live

text: Generic

text: Zea mays

text: pericarp

text: QAF


text: Genoplante

text: live

text: Generic

text: Zea mays

text: ear leaf

text: QAG


text: Genoplante

text: live

text: Generic

text: Zea mays

text: ear leaf

text: QAH


text: Genoplante

text: live

text: Generic

text: Zea mays

text: ear leaf

text: QAI


text: Genoplante

text: live

text: Generic

text: Zea mays

text: ear leaf

text: QAJ


text: Genoplante

text: live

text: Generic

text: Zea mays

text: ear leaf

text: QAK


text: Genoplante

text: live

text: Generic

text: Zea mays

text: ear leaf

text: QAL


text: Genoplante

text: live

text: Generic

text: Zea mays

text: ear leaf

text: QAM


text: Genoplante

text: live

text: Generic

text: Zea mays

text: pericarp

text: QAN


text: Genoplante

text: live

text: Generic

text: Zea mays

text: pericarp

text: QAO


text: Genoplante

text: live

text: Generic

text: Zea mays

text: aerial, root, whole plant

text: QAP


text: Genoplante

text: live

text: Generic

text: Zea mays

text: aerial, root, whole plant

text: QAQ


text: Genoplante

text: live

text: Generic

text: Zea mays

text: aerial, root, whole plant

text: QAR


text: Genoplante

text: live

text: Generic

text: Zea mays

text: aerial, root, whole plant

text: QAS


text: Genoplante

text: live

text: Generic

text: Zea mays

text: aerial, root, whole plant

text: QAT


text: Genoplante

text: live

text: Generic

text: Zea mays

text: aerial, root, whole plant

text: QAU


text: Genoplante

text: live

text: Generic

text: Zea mays

text: aerial, root, whole plant

text: QAV


text: Genoplante

text: live

text: Generic

text: Zea mays

text: aerial, root, whole plant

text: QAW


text: Genoplante

text: live

text: Generic

text: Zea mays

text: aerial, root, whole plant

text: QAY


text: Genoplante

text: live

text: Generic

text: Zea mays

text: aerial, root, whole plant

text: QAZ


text: Genoplante

text: live

text: Generic

text: Zea mays

text: endosperm

text: QBA


text: Genoplante

text: live

text: Generic

text: Zea mays

text: embryo

text: QBB


text: Genoplante

text: live

text: Generic

text: Zea mays

text: ovary

text: QBC


text: Genoplante

text: live

text: Generic

text: Zea mays

text: pedicel, whole kernel

text: QBD


text: Genoplante

text: live

text: Generic

text: Zea mays

text: roots extremities

text: QBE


text: Genoplante

text: live

text: Generic

text: Zea mays

text: ear leaf

text: QBF


text: Genoplante

text: live

text: Generic

text: Zea mays

text: aleuron layer

text: QBG


text: Genoplante

text: live

text: Generic

text: Zea mays

text: sheath (in)

text: QBH


text: Genoplante

text: live

text: Generic

text: Zea mays

text: pedicel

text: QBI


text: Genoplante

text: live

text: Generic

text: Zea mays

text: pollen

text: QBJ


text: Genoplante

text: live

text: Generic

text: Zea mays

text: embryo

text: QBK


text: Genoplante

text: live

text: Generic

text: Zea mays

text: seedling minus kernel

text: QBL


text: Genoplante

text: live

text: Generic

text: Zea mays

text: apex

text: QBM


text: Genoplante

text: live

text: Generic

text: Zea mays

text: pedicel, whole kernel

text: QBN


text: Genoplante

text: live

text: Generic

text: Zea mays

text: roots extremities

text: QBO


text: Genoplante

text: live

text: Generic

text: Zea mays

text: ear leaf

text: QBP


text: Genoplante

text: live

text: Generic

text: Zea mays

text: endosperm

text: QBQ


text: Genoplante

text: live

text: Generic

text: Zea mays

text: roots extremities

text: QBS


text: Genoplante

text: live

text: Generic

text: Zea mays

text: 3rd adult leaf

text: QCA


text: Genoplante

text: live

text: Generic

text: Zea mays

text: 3rd adult leaf

text: QCB


text: Genoplante

text: live

text: Generic

text: Zea mays

text: 3rd adult leaf

text: QCC


text: Genoplante

text: live

text: Generic

text: Zea mays

text: 3rd adult leaf

text: QCD


text: Genoplante

text: live

text: Generic

text: Zea mays

text: seedling minus kernel

text: QCE


text: Genoplante

text: live

text: Generic

text: Zea mays

text: seedling minus kernel

text: QCF


text: Genoplante

text: live

text: Generic

text: Zea mays

text: embryo

text: QCG


text: Genoplante

text: live

text: Generic

text: Zea mays

text: embryo

text: QCH


text: Genoplante

text: live

text: Generic

text: Zea mays

text: embryo

text: QCI


text: Genoplante

text: live

text: Generic

text: Zea mays

text: cell lignification part of the 6th leaf

text: QCJ


text: Genoplante

text: live

text: Generic

text: Zea mays

text: cell division part of the 6th leaf

text: QCK


text: Genoplante

text: live

text: Generic

text: Zea mays

text: apex

text: QCL


text: Genoplante

text: live

text: Generic

text: Zea mays

text: apex

text: QCM


text: Genoplante

text: live

text: Generic

text: Zea mays

text: apex

text: QCN


text: Genoplante

text: live

text: Generic

text: Zea mays

text: apex

text: QCO


text: Genoplante

text: live

text: Generic

text: Zea mays

text: whole plant

text: QCR


text: Genoplante

text: live

text: Generic

text: Zea mays

text: whole plant

text: QCS


text: Genoplante

text: live

text: Generic

text: f333 or f334

text: Zea mays

text: seedling minus kernel

text: QCT


text: Genoplante

text: live

text: Generic

text: f333 or f334

text: Zea mays

text: seedling minus kernel

text: QCU


text: Genoplante

text: live

text: Generic

text: mixture

text: Zea mays

text: mixture

text: directaflp


text: Genoplante

text: live

text: Generic

text: recital

text: Triticum aestivum

text: root


text: Kyoto University

text: live

text: Generic

text: Bright Yellow No.2

text: primer: M13 forward; mRNA obtained from lag, log and stationary phase cells

text: Nicotiana tabacum

text: pGEM-T easy

characteristic: vector


text: National Institute of Genetics, Center for Information Biology and DNA Data Bank of Japan

text: live

text: Generic

text: adult

text: Dugesia japonica

text: head

text: planarian head cDNA


text: Miyazaki University

text: live

text: Generic

text: Cyprinus carpio

text: barbel

text: Cyprinus carpio barbel


text: Forestry and Forest Products Research Institute

text: live

text: Generic

text: Cryptomeria japonica

text: male cone

text: Cryptomeria japonica male cone


text: live

text: Generic

text: Cryptomeria japonica

text: developing female cone

text: Cryptomeria japonica developing female cone


text: live

text: Generic

text: adult

text: Dugesia japonica

text: eye

text: planarian eye cDNA


text: National Institute of Agrobiological Sciences

text: live

text: Generic

text: 5 weeks

text: Sus scrofa

text: male

characteristic: sex

text: main and accessory olfactory bulb

text: full-length enriched pig cDNA library, main and accessory olfactory bulb


text: live

text: Generic

text: 5 weeks

text: Sus scrofa

text: male

text: dentate gyrus

text: full-length enriched pig cDNA library, dentate gyrus

characteristic: title


text: live

text: Generic

text: 5 weeks

text: Sus scrofa

text: male

text: hippocampus

text: full-length enriched pig cDNA library, hippocampus


text: live

text: Generic

text: 5 weeks

text: Sus scrofa

text: male

text: cerebral cortex

text: full-length enriched pig cDNA library, cerebral cortex


text: live

text: Generic

text: 5 weeks

text: Sus scrofa

text: male

text: cerebellum

text: full-length enriched pig cDNA library, cerebellum


text: live

text: Generic

text: T cell

text: Sus scrofa

text: full-length enriched pig cDNA library, T cell


text: live

text: Generic

text: 5 weeks

text: Sus scrofa

text: male

text: basal ganglia

text: full-length enriched pig cDNA library, basal ganglia


text: University of Maryland, College Park

text: live

text: Generic

text: Amphidinium carterae

text: EcoRI

text: NotI

text: CCMP 1314

characteristic: strain

text: Amphidinium carterae

text: modified pBluescript SK+


text: University of Miami

characteristic: INSDC first public

text: live

text: Generic

text: mixed cell type

text: Gulf Toadfish Lamda Zap Express Library constructed from liver excised from a 50 gram mature female obtained in Biscayne Bay, Florida, USA.

characteristic: description

text: sexually mature adult

text: liver

characteristic: organ

text: Opsanus beta

text: EcoRI

text: XhoI

text: female

text: Gulf Toadfish Lambda Zap Express cDNA Library


characteristic: External Id

text: The University of Georgia, Department of Plant Biology

text: live

text: Generic

text: The library was prepared from polyA+ RNA from seedlings grown hydroponically and in the absence of added iron. At 8 days of age, roots and shoots were harvested and stored at -80 C until RNA was isolated. Double-stranded cDNA was cloned unidirectionally into different DraIII sites of the pME18S-FL3 vector (5-prime DraIII site is CACTGTGTG, 3-prime DraIII site is CACCATGTG). XhoI excises the cDNA insert.

text: DH10B-T1 phage-resistant E. coli

characteristic: lab-host

text: Sorghum bicolor

text: XhoI

text: XhoI

text: Iron-deficient seedlings

text: Plasmid


text: Commonwealth Scientific and Industrial Research Organisation Division of Plant Industry

characteristic: INSDC center name

characteristic: INSDC last update

text: live

text: Generic

text: mRNA was prepared from prepared using polyA+ mRNA isolated from Arabidopsis hairy root cultures (ecotype C24) induced with Agrobacterium rhizogenes. The roots were treated with low-oxygen for 4 hours in liquid Murashige and Skoog (MS) medium, incubated in a 5% O2/95% N2 gas mixture. The root cultures were treated with 10 uM of the protein synthesis inhibitor cycloheximide, 2 h prior to and during the low-oxygen treatment.

text: root

text: Arabidopsis thaliana

text: Hairy root culture

text: Phage

text: Lambda ZIPLOX


text: Universite de Neuchatel

text: live

characteristic: INSDC status

text: Generic

text: Chasselas

text: Leaf

text: Vitis vinifera

text: Plasmid

text: pGEM-T Easy


text: Department of Plant Genetic Engineering, Dong-A University

text: live

text: Generic

text: After the suppression subtractive hybridization, cDNA fragments induced by wound treatment and fungal elicitor were cloned by TA cloning method, and screened by microarray.

text: Wound treatment and Fungal elicitor & vegetative stage

characteristic: dev-stage

text: Oryza grandiglumis

text: leaf

text: Oryza grandiglumis Subtracted cDNA library

text: Phagemid


text: University of Georgia

text: live

text: Generic

characteristic: NCBI submission model

text: Gossypium hirsutum

text: Leaves

text: Wilt Resistance

text: Plasmid

text: T-easy


text: Alfred-Wegener-Intitute for Polar and Marine Research

text: live

text: Generic

characteristic: NCBI submission package

text: total polyA was used for first-strand synthesis with SMART IV oiligos and CDS III/3'PCR primer. Double strand cDNA synthesis was done by LD PCR using the following program: 95oC for 5 min denaturation and subsequent 20 cycles at 95oC (2min) and 68oC (6min). After SfiI digestion the cDNA was fractionated with CHROMA Spin-400 columns. These cDNAs were ligated overnight into pTriplEX2 vectors.

text: Fragilariopsis cylindrus

text: Antarctic

text: Fragilariopsis cylindrus SMART cDNA library (Clontech)

text: Phage


text: Institute of Himalayan Bioresource Technology

text: live

text: Generic

text: Camellia sinensis

characteristic: organism

text: leaf

text: Camellia sinensis leaf


text: University of California at Davis (UCD)

text: live

text: Generic

text: The library was constructed from four different sources (seedling, root, leaf and flower) of RNA from a single genotype. cDNAs were pooled and directionally cloned into a custom medium-copy vector. Details of library construction can be obtained at http://cgpdb.ucdavis.edu/

text: E.coli

text: Helianthus paradoxus

text: QH_K sunflower H.paradoxus

text: Plasmid

text: pBRcDNASfiAB


text: University of California at Davis (UCD)

text: live

text: Generic

text: The library was constructed from four different sources (seedling, root, leaf and flower) of RNA from a single genotype. cDNAs were pooled and cloned into a high-copy vector pGEM-T. Details of library construction can be obtained at http://cgpdb.ucdavis.edu/

text: E.coli

text: Helianthus paradoxus

text: QH_L sunflower H.paradoxus (salt stress)

text: Plasmid

text: pGEM-T


text: University of California at Davis (UCD)

text: live

text: Generic

text: The library was constructed from three different sources (seedling, root and leaf) of RNA from a single genotype. cDNAs were pooled and directionally cloned into a custom medium-copy vector. Details of library construction can be obtained at http://cgpdb.ucdavis.edu/

text: E.coli

text: Helianthus argophyllus

text: QH_M sunflower H.argophyllus

text: Plasmid

text: pBRcDNASfiAB


text: University of California at Davis (UCD)

text: live

text: Generic

text: The library was constructed from three different sources (seedling, root and leaf) of RNA from a single genotype. cDNAs were pooled and cloned into a high-copy vector pGEM-T. Details of library construction can be obtained at http://cgpdb.ucdavis.edu/

text: E.coli

text: Helianthus argophyllus

text: QH_N sunflower H.argophyllus (drought stress)

text: Plasmid

text: pGEM-T


text: University of California

text: live

text: Generic

text: Assembly of sequence reads of overlapping cDNA clones

text: Asexual sporangia from hyphae grown on rye agar

text: Phytophthora infestans

text: SalI

text: NotI

text: Undifferentiated asexual sporangia

text: plasmid


text: University of California, Davis

text: live

text: Generic

text: mRNA was isolated from microdissected rat lung airways and parenchyma tissues.

text: adult

text: lung

text: Rattus norvegicus

text: Eco RI

text: Not I

text: male

text: Sprague-Dawley

text: airway or parenchyma

characteristic: tissue-type

text: Rat lung airway and parenchyma cDNA libraries

text: plasmid


text: Michigan Technological University, School of Forest Resources & Environmental Science

text: live

text: Generic

text: leaf

text: Populus tremuloides

text: Aspen leaf mRNA differential display


text: West China Hospital, Sichuan University

text: live

text: Generic

text: oocytes

characteristic: cell type

text: Xenopus laevis

text: EcoRI

text: XbaI

text: Xenopus laevis Lambda TripleEx Express Library

text: phage

text: pTripleEx

characteristic: vector


text: Children's Memorial Research Center

text: live

text: Generic

text: The library was constructed according Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. Denatured RNA was size fractionated on a 1% agarose gel. First strand cDNA synthesis was primed with oligo-dT primer containing a Not I site. Double strand cDNA was size selected according to mRNA size fraction, ligated with EcoR I adaptor, digested with Not I and then cloned directionally into pT7T3 Pac vector. The library tag sequence located between the Not I site and the polyA tail is CCCAC. Tissue was provided by Tim Ratlift.

text: DH10B (T1 phage resistant)

text: Prostate

text: Homo sapiens

text: EcoR I

text: Not I

text: CNCAP(3)T-225 cell line

characteristic: title

text: Plasmid

text: pT7T3 Pac


text: Children's Memorial Research Center

text: live

text: Generic

text: The library was constructed according Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. Denatured RNA was size fractionated on a 1% agarose gel. First strand cDNA synthesis was primed with oligo-dT primer containing a Not I site. Double strand cDNA was size selected according to mRNA size fraction, ligated with EcoR I adaptor, digested with Not I and then cloned directionally into pT7T3 Pac vector. The library tag sequence located between the Not I site and the polyA tail is TAGA. Tissue was provided by Tim Ratlift.

text: DH10B (T1 phage resistant)

text: Prostate

text: Homo sapiens

text: EcoR I

text: Not I

text: CNCAP(3)T-225 cell line

text: Plasmid

text: pT7T3 Pac


text: National Cancer Institute / NIH

text: live

text: Generic

text: The library was constructed according Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. Denatured RNA was size fractionated on a 1% agarose gel. First strand cDNA synthesis was primed with oligo-dT primer containing a Not I site. Double strand cDNA was size selected according to mRNA size fraction, ligated with EcoR I adaptor, digested with Not I and then cloned directionally into pYX-Asc vector. The library tag sequence located between the Not I site and the polyA tail is GATAAGGCCA. Tissue was provided by Mary Hendrix.

text: DH10B (T1 phage resistant)

text: Lung

text: Homo sapiens

text: EcoR I

text: Not I

text: Chondrosarcoma Lung Metastasis cell lines

text: Plasmid

text: pYX-Asc


text: Children's Memorial Research Center

text: live

text: Generic

text: DH10B (T1 phage resistant)

text: Lung

text: Homo sapiens

text: EcoR I

text: Not I

text: Plasmid

text: pYX-Asc


text: live

text: Generic

text: Screening from a subtracted embryonic E6 ventral neuroepithelial cDNA library Chick embryonic ventral neuroepithelial cDNA cloned into pT-Adv using T/A - based cloning system (AdvanTAge PCR cloning kit, Clontech)

text: Hamburger Hamilton stage 29-30

text: Gallus gallus

text: ventral neuroepithelium

text: Chick ventral neuroepithelium cDNA library

text: pT-Adv


text: US Department of Agriculture, Agriculture Research Service, Pacific West Area, Western Regional Research Center

text: live

text: Generic

text: Chinese Spring

characteristic: cultivar

text: Plants were grown in a glasshouse. Anther meiotic stage was determined microscopically after removing a single anther from a primary floret. If determined to be between (and including) meiotic stages pre-meiosis and metaphase I, remaining floret tissue was collected and pooled for library construction. The tissue, total RNA, and poly(A) RNA were prepared, cDNA synthesised, and directionally ligated into pSPORT1 by Tim Sutton in the P Langridge Lab at the Department of Plant Science, University of Adelaide, Waite Campus, Australia. Average insert size 1.4Kb. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

text: Meiotic stages pre-meiosis-metaphase I

text: E. coli DH10B

text: Triticum aestivum

text: SalI

text: NotI

text: Whole florets with anthers

text: Wheat meiotic floret cDNA library

text: Plasmid


text: University of Georgia

characteristic: INSDC first public

text: live

text: Generic

text: Gossypium barbadense

text: Leaves

text: NBS

text: Plasmid

text: T-easy


text: National Institute on Aging/National Institutes of Health

text: live

text: Generic

text: Mouse cDNA project by the Laboratory of Genetics, National Institute on Aging (NIA), Intramural Research Program, NIH (http://lgsun.grc.nia.nih.gov/cDNA). In brief, double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 26 ug of total RNA, treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-S. The products were purified by phenol/chloroform and Centricon 100. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pCMV-SPORT6 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 3.0 kb. The library was constructed by Yulan Piao.

characteristic: description

text: Newborn Kidney

text: Mus musculus

text: SalI

text: NotI

characteristic: strain

text: NIA Mouse Newborn Kidney cDNA Library (Long 1)

text: plasmid (ampicillin resistant)

text: pCMV-SPORT6 (Invitrogen)


characteristic: External Id

text: live

text: Generic

text: Mouse cDNA project by the Laboratory of Genetics, National Institute on Aging (NIA), Intramural Research Program, NIH (http://lgsun.grc.nia.nih.gov/cDNA). This is a long-transcript enriched cDNA library (Ref. Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]). Total RNAs were extracted from a pool of 1488 unfertilized eggs. Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'], treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-S. The products were purified by phenol/chloroform and Centricon 100. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pCMV-SPORT6 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 2.5 kb. The library was constructed by Yulan Piao.

text: Unfertilized Egg

text: Mus musculus

text: SalI

text: NotI

text: NIA Mouse Unfertilized Egg cDNA Library (Long 1)

text: plasmid (ampicillin resistant)

text: pCMV-SPORT6 (Invitrogen)


text: Janssen Research Foundation

text: live

text: Generic

text: Candida albicans

text: Candida albicans library (De Backer MD)


text: MPI fuer Zuechtungsforschung

text: live

text: Generic

text: Antirrhinum majus

text: Antirrhinum majus library (Stueber K)


text: MPI fuer Zuechtungsforschung

text: live

text: Generic

text: Antirrhinum majus

text: petal

text: Antirrhinum majus petal


text: University Of Tuebingen

text: live

text: Generic

text: country:United Kingdom

text: early development

characteristic: isolate

text: Funneliformis mosseae

text: Glomus mosseae early development


text: University of Padua

characteristic: INSDC center name

text: live

text: Generic

text: Homo sapiens

text: female

characteristic: sex

text: pectoral muscle (after mastectomy)


text: The Sanger Institute The Wellcome Trust Genome Campus

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: country: Zimbabwe; EST from adult gut infected with T.brucei

text: Glossina morsitans morsitans

text: adult infected gut

text: Glossina morsitans morsitans adult infected gut


text: Institut fuer Gemuese- und Zierpflanzenbau

text: live

text: Generic

characteristic: NCBI submission model

text: Daucus carota

characteristic: lab-host

text: Rhizophagus intraradices

text: extraradical mycelium

text: Glomus intraradices extraradical mycelium


text: University of Parma

text: live

text: Generic

characteristic: NCBI submission package

text: Georgie

text: drought-stressed

text: Hordeum vulgare subsp. vulgare

characteristic: organism

text: coleoptile

text: Hordeum vulgare subsp. vulgare coleoptile


text: live

text: Generic

text: The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. Denatured mRNa was size fractionated on a 1% agarose gel. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was size selected according to mRNA size fraction, ligated with EcoR I adaptor, digested with Not I, and then cloned directionally into pYX-Asc vector. The library tag sequence located between the Not I site and the polyA tail, is GTGCGTGGAA. This library was created for the University of Iowa Mouse Brain Molecular Anatomy Project (BMAP): 'Gene Discovery in the Developing Mouse Nervous System', supported by National Instututes of Mental Health (NIMH), Hemin Chin, Ph.D., program coordinator.

text: embryo 15.5 dpc

characteristic: dev-stage

text: DH10B (T1 phage resistant)

text: brain

characteristic: organ

text: Mus musculus

text: EcoR I

text: Not I

text: whole brain

characteristic: tissue-type

text: Plasmid

text: pYX-Asc


text: USDA - ARS

text: live

text: Generic

text: A high quality EST with at least 100 contiguous bases at Trace Tuner score of 20 or better.

text: Adult

text: Homalodisca vitripennis

text: EcoRI

text: XhoI

text: Mixed population

text: Entire insect

text: Adult Glassy-winged Sharpshooter (WHHc)

characteristic: title

text: pBluescript II SK+

characteristic: vector


text: Richard Stockton College

characteristic: INSDC first public

text: live

text: Generic

text: Differentially expressed clones from reciprocal SSH libraries comparing fish from pollution impacted habitats versus less impacted habitats.

characteristic: description

text: adult

text: Liver

text: Pseudopleuronectes americanus

text: mix of female and male

text: Winter Flounder SSH

text: Plasmid

text: pGEM-T


characteristic: External Id

text: Development Center for Biotechnology and Academia Sinica

text: live

text: Generic

text: sporophyte

text: Marsilea quadrifolia

text: vegetative shoot apex

text: Marsilea quadrifolia vegetative shoot apex sporophyte


text: Tokyo Medical and Dental University, Medical Research Institute

text: live

text: Generic

text: hepatocytes

characteristic: cell type

text: liver

text: Rattus norvegicus

text: male

text: Fisher 344

characteristic: strain

text: liver

text: Rattus norvegicus liver Fisher 344


text: Marine Biological Laboratory

characteristic: INSDC center name

text: live

text: Generic

text: Argopecten irradians

text: adductor muscle

text: Bay Scallop ZAP Express Library

text: pBK-CMV


text: Northeast Forestry University

characteristic: INSDC last update

text: live

text: Generic

text: Puccinellia tenuiflora

text: leaf

text: ESTs of Puccinellia tenuiflora


text: UC Davis, Plant Pathology

text: live

characteristic: INSDC status

text: Generic

text: RR890915N is a cDNA library of leaves from the F1 of Vitis rupestris 'A. de Serres' x V. spp. 'b42-26' (8909-15) showing resistance to Xylella fastidiosa under greenhouse experimental conditions (M. Andrew Walker and Alan Krivanek, U C Davis). Samples were collected from 17-week old greenhouse grown plants. cDNAs were made by oligo-dT priming and directionally cloned. 5'and 3' adaptors were used in cloning as follows: 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' and 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)NN-3'. Library was constructed using the Clontech Creator SMART kit and size-selected to contain the 0.5-3 kb size fraction.

text: Leaf

text: Vitis hybrid cultivar

text: SfiI

text: SfiI

text: Vitis sp. RR890915N

text: Plasmid

text: pDNR


text: UC Davis, Plant Pathology

text: live

text: Generic

characteristic: NCBI submission model

text: RR890915I is a cDNA library of leaves from the F1 of Vitis rupestris 'A. de Serres' x V. spp. 'b42-26' (8909-15) showing resistance to Xylella fastidiosa under greenhouse experimental conditions (M. Andrew Walker and Alan Krivanek, U C Davis). Samples were collected from 17-week old greenhouse grown plants, nine weeks after inoculation with the bacterial pathogen Xylella fastidiosa. cDNAs were made by oligo-dT priming and directionally cloned. 5'and 3' adaptors were used in cloning as follows: 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' and 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)NN-3'. Library was constructed using the Clontech Creator SMART kit and size-selected to contain the 0.5-3 kb size fraction.

text: Leaf

text: Vitis hybrid cultivar

text: SfiI

text: SfiI

text: Vitis sp. RR890915I

text: Plasmid

text: pDNR


text: UC Davis, Plant Pathology

text: live

text: Generic

text: Cabernet Sauvignon

characteristic: cultivar

text: CAST is a cDNA library of Vitis vinifera cv. 'Cabernet Sauvignon' stems. Samples were collected May 13, 2002 from pre-bloom plants (10-11 days before bloom), pre-veraison. Sampled vines were located at the University of California, Davis, Experimental Vineyard. cDNAs were made by oligo-dT priming and directionally cloned. 5'and 3' adaptors were used in cloning as follows: 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' and 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)NN-3'. Library was constructed using the Clontech Creator SMART kit and size-selected to contain the 0.5-3 kb size fraction.

text: Pre-bloom (10-11 days before bloom)

characteristic: lab-host

text: Stem

text: Vitis vinifera

text: sfiI

text: sfiI

text: Hermaphrodite

characteristic: sex

text: Vitis vinifera cv. cabernet sauvignon Stem - CAST

text: Plasmid

text: pDNR


text: University of New Mexico

text: live

text: Generic

characteristic: NCBI submission package

text: mRNA, pooled from 7 untreated M-line B. glabrata snails, was used as template for RT-PCR with random primers (25-28nt, at least 50% GC) to generate ORESTES(ORF-ESTs). Resulting cDNAs were shotgun cloned (pCR2.1 TOPO TA), and sequenced using M13 primers. camco = C Adema M-line Control Orestes. Random primers were removed from sequence as presented.

text: adult

characteristic: dev-stage

text: Biomphalaria glabrata

characteristic: organism

text: hermaphrodite

text: M-line

text: whole body

text: camco 09-03

text: Plasmid


text: Australian National University

text: live

text: Generic

text: Onchocerca volvulus

text: Onchocerca volvulus library (Pellerone FI)

characteristic: title


text: Australian National University

text: live

text: Generic

text: Brugia malayi

text: Brugia malayi library (Pellerone FI)


text: University of Birmingham

text: live

text: Generic

text: country: Isle of Man:Port Erin

text: sexually immature

text: Platichthys flesus

text: liver

characteristic: tissue-type

text: Platichthys flesus liver sexually immature


text: Niigata University

characteristic: INSDC first public

text: live

text: Generic

text: Cryptomeria japonica

text: Cryptomeria japonica library (Moriguchi Y)


text: University of Tuebingen

text: live

text: Generic

text: 5 days post fertilisation larva

text: Danio rerio

text: whole larva

text: Danio rerio whole larva 5 days post fertilisation larva


text: University of Birmingham

text: live

text: Generic

text: country: United Kingdom:Port Erin, Isle of Man; subtracted cDNA library

characteristic: description

text: immature

text: Platichthys flesus

text: liver

text: Platichthys flesus liver immature, subtracted cDNA library


characteristic: External Id

text: Institute of Parasitic Diseases

text: live

text: Generic

text: Schistosoma japonicum

text: Schistosoma japonicum mRNA (Li X)


text: National Cancer Institute / NIH

text: live

text: Generic

text: The library was constructed by reverse transcription of 1 ug mRNA using the oligo dT primer GCGGCCGCCC(T)20 and an RNaseH + MMLV reverse transcriptase. Second strand synthesis was carried out by standard methods. The cDNA was size selected by agarose gel for > 1.2 kb, digested with Not I and directionally cloned into the vector Express-1 at the SmaI/NotI sites. DNA from the primary library was used for in vitro transcription from the T7 promoter to produce biotinylated RNA transcripts. These biotinylated transcripts, along with blocking oligos to the poly-A, multiple cloning site and primer regions, were hybridized with single stranded circles produced by phagmeid production from the primary library to a Cot value of 10-20. Strepavidin/phenol extraction was utilized to remove DNA:RNA hybrids leaving un-hybridized single stranded circles which were repaired by primer extension and transformed back into E. coli resulting in the normalized library. Average insert size 2.0 kb. 3' linker/adaptor sequence GCGGCCGCCC(T)20. This libary was constructed by Agencourt Bioscience.

text: DH10B (T1 phage-resistant)

text: Homo sapiens

text: SmaI

text: NotI

text: Brain glioblastoma

text: plasmid (ampicillin-resistant)

characteristic: vector


text: National Cancer Institute / NIH

text: live

text: Generic

text: Bulk tissue was collected from a whole adult individual from the Tuebingen strain. 1st strand cDNA was primed with a Not I - oligo(dT) primer, double-stranded cDNA was cloned into the Not I and EcoRV sites of pExpress-1. Library was size-selected for >1 kb fragments and normalized. A non-normalized version of this library is also available (NIH_ZGC_10). Library was constructed by Open Biosystems (Huntsville, AL)

text: Danio rerio

text: NotI

text: EcoRV

text: whole body

text: phagemid (ampicillin resistant)


text: Geron Corporation

text: live

text: Generic

text: oligo dT primed, full-length enriched cDNA library from undifferentiated hES cell lines H1 (p32), H7 (p29), and H9 (p26) maintained in feeder-free conditions

text: Homo sapiens

text: embryonic stem cells, cell lines H1, H7, and H9


text: Geron Corporation

text: live

text: Generic

text: oligo dT primed, full-length enriched cDNA library from embryoid body outgrowths derived from hES cell lines H1 (p32), H7 (p29), and H9 (p26) maintained in feeder-free conditions.

text: Homo sapiens

text: embryonic stem cells, embryoid bodies derived from H1, H7 and H9 cells


text: Geron Corporation

text: live

text: Generic

text: oligo dT primed, full-length enriched cDNA library from DMSO-treated hES cell line H9 (p22) maintained in feeder-free conditions

text: Homo sapiens

text: embryonic stem cells, DMSO-treated H9 cell line


text: Geron Corporation

text: live

text: Generic

text: oligo dT primed, full-length enriched cDNA library from hES cell line H7 (p29) maintained in feeder-free conditions. Embryoid bodies were generated in the presence of all-trans retinoic and mitogens.

text: Homo sapiens

text: embryonic stem cell, retinoic acid and mitogen-treated hES cell line H7


text: University of Ghent

text: live

text: Generic

text: Bos taurus

text: Pooled cDNA


text: Washington University School of Medicine

text: live

text: Generic

text: 1st strand of cDNA was synthesized with reverse transcriptase and oligo(dT) beads, then cDNA was amplified by PCR using modified SMART primers. The final cDNA was cloned in pAMP1 vector in annealing reaction with Uracil DNA Glycosylase (UDG). Library materials provided by T.S. Stappenbeck and J.I. Gordon. Library constructed by R. Tidwell Y.Korshunova, M. Lovett. Library was re-arrayed by R. Tidwell.

text: XL1 Blue MRF'

text: Mus musculus

text: Laser-captured colonic epithelial progenitors cells from conventionally raised C57/Bl6 mice

text: Normalized colon epithelial progenitors cDNA library

text: phagemid


text: Harvard University, Howard Hughes Medical Institute

text: live

text: Generic

text: Total RNA was isolated from the common pancreatic duct with some surrounding tissue obtained 2 and 3/4th day following 90% partial pancreatectomy. Pancreatic tissue used for the RNA isolation represents the focal region of pancreatic regeneration. Full-length cDNA was prepared from the total RNA using smart RACE cDNA amplification kit from Clontech, based on the manufacturers suggested protocol 5' end of the cDNAs contained a Sfia site, while 3' end contained a Sfib site. Kanamycin resistance gene from pEGFP (Clontech) was amplified using PCR primers with 5' primer containing HindIII site followed by Sfia site, and 3' primer contained Sfib site followed by EcoR1 site. PCR amplified Kan resistance gene was cloned into HindIII-EcoR1 cut pcDNA3.1Zeo(+) vector, digesting this plasmid with Sfi releases the Kan resistance insert. Resulting Sfi cut vector, when ligated with Sfi cut smart RACE amplified cDNA will result in directional cloning (5'-3') of the cDNA insert down-stream from the CMV promoter. The cDNA library was constructed in a collaborative project between Dr Arun Sharma and Dr. Susan Bonner-weir; other individuals involved in this project were Petra Reitz, Julie Weitekamp, Monica Taneja and Kiirsten Suurkask.

text: 2 and 3/4th days following 90% partial pancreatectomy

text: E. coli XL-10 Gold

text: Rattus norvegicus

text: HindIII

text: Male

text: Sprague-Dawley

characteristic: strain

text: Common Pancreatic duct and surrounding tissue

text: Joslin Regenerating Pancreas full-length cDNA library

text: mammalian expression vector (ampicillin resistant)

text: pcDNA3.1 Zeo(+)


text: CNRS

characteristic: INSDC center name

text: live

text: Generic

text: Spleen

characteristic: organ

text: Gallus gallus

text: Marek's disease virus-infected spleen


text: CNRS

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Splenocytes

characteristic: cell type

text: Gallus gallus

text: PHA-activated splenocytes


text: CNRS

text: live

text: Generic

characteristic: NCBI submission model

characteristic: cell-line

text: Gallus gallus

text: LPS-activated macrophage cell line


text: University of Notre Dame

text: live

text: Generic

characteristic: NCBI submission package

text: a directionally cloned and normalized, oligo-T primed cDNA library constructed from equal numbers of 24 and 40 hr post Plasmodium inui infection, 4arr and L35 strain adult female mosquito abdomens (omitting ovaries and midguts): Bonaldo, Lennon & Soares (1996): Normalization and Subtraction: Two Approaches To Facilitate Gene Discovery, Genome Research 6, 791-806. ESTs sequenced from the M13 reverse priming site reading from the 5' ends of the cDNAs are indicated by 'R' in the clone name.

text: E. coli DH10B

characteristic: lab-host

text: Anopheles gambiae

text: EcoR1 (5'end)

text: Not1 (3'end)

text: Normalized Anopheles Fat Body (NAFB) Library


text: University of Notre Dame

text: live

text: Generic

text: a directionally cloned and normalized, oligo-T primed cDNA library constructed from strain 4arr adult mosquito heads. Equal numbers of sugar fed males, sugar fed females and 6, 24 and 48 hr post blood meal females were used: Bonaldo, Lennon & Soares (1996): Normalization and Subtraction: Two Approaches To Facilitate Gene Discovery, Genome Research 6, 791-806. ESTs sequenced from the M13 reverse priming site reading from the 5' ends of the cDNAs are indicated by 'R' in the clone name. ESTs sequenced from the M13 forward priming site reading from the 3' ends of the cDNAs are indicated by 'F' in the clone name.

text: E. coli DH10B

text: Anopheles gambiae

characteristic: organism

text: EcoR1 (5'end)

text: Not1 (3'end)

text: Normalized Anopheles Head (NAH) Library


text: University of Washington

text: live

text: Generic

text: To reveal differences in gene expression between adult and juvenile phases of Acetabularia acetabulum, we constructed two subtracted EST libraries using suppressive subtractive hybridization. One library is putatively enriched in adult-specific transcripts and the other is putatively enriched in juvenile-specific transcripts. The two mRNA samples used to create these libraries were isolated from Acetabularia acetabulum whole late adult and early reproductive unicells and from whole Acetabularia acetabulum juvenile unicells. This EST was obtained from the adult-specific library.

text: late adult and early reproductive

characteristic: dev-stage

text: Acetabularia acetabulum

text: Aa0005 (=Ladenburg 17)

text: whole unicells

text: Adult-specific

characteristic: title


text: University of Washington

characteristic: INSDC first public

text: live

text: Generic

text: To reveal differences in gene expression between adult and juvenile phases of Acetabularia acetabulum, we constructed two subtracted EST libraries using suppressive subtractive hybridization. One library is putatively enriched in adult-specific transcripts and the other is putatively enriched in juvenile-specific transcripts. The two mRNA samples used to create these libraries were isolated from Acetabularia acetabulum whole late adult and early reproductive unicells and from whole Acetabularia acetabulum juvenile unicells. This EST was obtained from the juvenile-specific library.

text: juvenile

text: Acetabularia acetabulum

text: Aa0005 (=Ladenburg 17)

text: whole unicells

characteristic: tissue-type

text: Juvenile-specific


characteristic: External Id

text: Wageningen University and Research Centre

text: live

text: Generic

text: Dover

characteristic: cultivar

text: mRNA, isolated from carnation petals from various developmental stages, was pooled and subtracted using the Clontech PCR-Select cDNA Subtraction Kit. Stem tissue (located just under the flower head) was used as the driver. The pGEM-T Easy Vector System (Promega) was used to construct a cDNA library from the resulting fragments.

characteristic: description

text: E. coli JM109

text: Dianthus caryophyllus

text: petal

text: subtracted carnation petal cDNA library

text: pGEM-T Easy


text: Washington University School of Medicine

characteristic: INSDC center name

text: live

text: Generic

text: The cDNA library was constructed by Keliang Tang, and Robert Cole at Washington University. cDNA was synthesized from Poly(A)+ mRNA using an oligo-d(T) primer containing a XhoI site. Following second strand synthesis, EcoRI adapters were ligated to the cDNA, and products were size-selected on sephacryl S500. The cDNA were directionally cloned into the EcoRI/XhoI prepared pBluescript II SK+ vector, and electroporated into ElectroTen Blue cells (Stratagene). The library may contain a small percentage of host or bacterial contaminants.

text: Tachyzoite

text: ElectroTen Blue cells (Stratagene)

text: Neospora caninum

text: EcoRI

text: XhoI

text: Nc-LIV Tachyzoite cDNA Library

text: pBluescript II SK+Vector_type: plasmid

characteristic: vector


text: Chang Gung University

characteristic: INSDC last update

text: live

text: Generic

text: Danio rerio

text: Danio rerio library (Wu K-M)


text: University of Catania

text: live

characteristic: INSDC status

text: Generic

text: fetal

text: Homo sapiens

text: brain

text: Homo sapiens brain fetal


text: Auburn University

text: live

text: Generic

text: Intestine

characteristic: organ

text: Ictalurus punctatus

text: NotI

text: SalI

text: Intestine cDNA library

text: Phagemid


text: Auburn University

text: live

text: Generic

characteristic: NCBI submission model

text: Muscle

text: Ictalurus punctatus

text: NotI

text: SalI

text: Muscle cDNA library

text: Phagemid


text: Auburn University

text: live

text: Generic

text: Ovary

text: Ictalurus punctatus

text: NotI

text: SalI

text: Ovary cDNA library

text: Phagemid


text: Auburn University

text: live

text: Generic

text: Pituitary

text: Ictalurus punctatus

text: NotI

text: SalI

text: Pituitary cDNA library

text: Phagemid


text: Auburn University

text: live

text: Generic

text: Stomach

text: Ictalurus punctatus

text: NotI

text: SalI

text: Stomach cDNA library

text: Phagemid


text: Auburn University

text: live

text: Generic

text: Testis

text: Ictalurus punctatus

text: NotI

text: SalI

text: Testis cDNA library

text: Phagemid


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: Mus musculus

text: pBluescript Lion


text: FSU Jena

text: live

text: Generic

text: Quercus robur

text: Quercus robur library (Oelmueller R)


text: Oklahoma State University

text: live

text: Generic

text: embryonic fibroblast

characteristic: cell type

text: Rat1 + ER alpha

characteristic: cell-line

text: Rattus norvegicus

text: Rat1 tester Rat1 + ER beta driver Suppression Subtractive Hybridization Library


text: Oklahoma State University

text: live

text: Generic

characteristic: NCBI submission package

text: embryonic fibroblast

text: Rat1 + ER alpha

text: Rattus norvegicus

text: Rat1 + ER beta tester Rat1 driver Suppression Subtractive Hybridization Library


text: Oklahoma State University

text: live

text: Generic

text: embryonic fibroblast

text: Rat1 + ER alpha

text: Rattus norvegicus

characteristic: organism

text: Rat1 + ER beta vehicle Suppression Subtractive Hybridization Library


text: Oklahoma State University

text: live

text: Generic

text: embryonic fibroblast

text: Rat1 + ER alpha

text: Rattus norvegicus

text: Rat1 tester Rat1+ ER alpha driver Suppression Subtractive Hybridization Library


text: Oklahoma State University

text: live

text: Generic

text: embryonic fibroblast

text: Rat1 + ER alpha

text: Rattus norvegicus

text: Rat1 + ER alpha tester Rat1 driver Suppression Subtractive Hybridization Library


text: Cornell University

text: live

text: Generic

text: subtraction library of ripe fruit vs. leaves

text: Capsicum chinense

text: Capsicum chinense subtraction library

text: pCR 2.1 (Invitrogen)


text: Ludwig Institute for Cancer Research

text: live

text: Generic

text: pool of tumor cell lines

text: This sequence corresponds to an RT-PCR fragment generated within the Transcript Finishing Initiative (TFI), which is devoted to the characterization of new human transcripts. Using the genomic sequence as a scaffold for EST mapping and clustering we have performed RT-PCR to bridge gaps between EST clusters that are likely to be derived from the same genes. Each pair of EST clusters selected for experimental validation was designated a single Transcript Finishing Unit (TFU). In this way we are able to confirm the membership of ESTs from different clusters to a common transcript and to provide intervening sequence information.

text: Homo sapiens

text: Transcript Finishing Unit (TFU)


text: Emory University

text: live

text: Generic

text: The Rat Lambda Zap 4 h light-exposed retina cDNA library was constructed from retinal RNA isolated from weanling male albino Sprague-Dawley rats, which were maintained in darkness for 40 days. At 61 days of age rats were exposed to intense visible light (490-580 nm; 1200 to 1400 lux) for 4 hours.

text: adult

characteristic: dev-stage

text: Rattus norvegicus

text: male

characteristic: sex

text: Sprague-Dawley

characteristic: strain

text: retina

text: Phage

text: Uni-ZAP XR


text: Shizuoka University

characteristic: INSDC first public

text: live

text: Generic

text: Miyagawa-wase

text: maturation stage

text: Citrus unshiu

text: juice sac and pulp segment

characteristic: tissue-type

text: Citrus unshiu juice sac and pulp segment maturation stage

characteristic: title


characteristic: External Id

text: live

text: Generic

text: Gametocytes of Gregarina niphandrodes were obtained from Tenebrio molitar by Dr. Charlotte Omoto (Washington State University). The cDNA library was constructed by Keliang Tang, and Robert Cole at Washington University. cDNA was synthesized from poly(A)+ mRNA using the template-switching PCR method (SMART cDNA Kit, BD Biosciences). First strand cDNA was reverse transcribed using the CDS III/3' primer and a 5' template switch primer (Smart IV primer). The product of the first strand synthesis was PCR amplified using the same primer set and the fragments were digested with SfiI. The fragments were size selected, ligated into a modified pBluescript vector (obtained from Michael White, Montana State University) containing directional SfiI sites, and electroporated into ElectroTen Blue cells. Vector: SfiI sites were added to the multiple cloning region of pBluescript SK+ between the BamHI/EcoRI sites. The modified polylinker has the following sequence: 5'GAATTCGGCCATTACGGCC(G)n-- insert-- GGCCGCCTCGGCCCACGGATCC3' where n=3-4 G nucleotides.

characteristic: description

text: Gametocytes

text: ElectroTen cells (Stratagene)

characteristic: lab-host

text: Gregarina niphandrodes

text: Gregarina niphandrodes WSU-1 gametocytes cDNA Library

text: plasmid

text: Modified pBluescript (pBS SK+)


text: Central China Normal University

characteristic: INSDC center name

characteristic: INSDC last update

text: live

text: Generic

text: Trichoplusia ni

text: Trichoplusia ni cell line with high resistance to Cry1Ac toxin


text: Central China Normal University

text: live

characteristic: INSDC status

text: Generic

text: cell line susceptible to Cry1Ac toxin

text: Trichoplusia ni

text: Trichoplusia ni cell line susceptible to Cry1Ac toxin


text: National Cancer Institute / NIH

text: live

text: Generic

characteristic: NCBI submission model

text: Bulk tissue was collected from a whole adult individual from the Tuebingen strain. 1st strand cDNA was primed with a Not I - oligo(dT) primer, double-stranded cDNA was cloned into the Not I and EcoRV sites of pExpress-1. Library was size-selected for >1 kb fragments. A normalized version of this library is also available (NIH_ZGC_7). Library was constructed by Open Biosystems (Huntsville, AL).

text: Danio rerio

text: NotI

text: EcoRV

text: whole body

text: phagemid (ampicillin resistant)

characteristic: vector


text: Washington University School of Medicine

text: live

text: Generic

text: The cDNA library was constructed by Keliang Tang, and Robert Cole at Washington University. cDNA was synthesized from poly(A)+ mRNA using the template-switching PCR method (SMART cDNA Kit, BD Biosciences). First strand cDNA was reverse transcribed using the CDS III/3' primer and a 5' template switch primer (Smart IV primer). The product of the first strand synthesis was PCR amplified using the same primer set and the fragments were digested with SfiI. The fragments were size selected, ligated into a modified pBluescript vector (obtained from Michael White, Montana State University) containing directional SfiI sites, and electroporated into ElectroTen Blue cells. Vector: SfiI sites were added to the multiple cloning region of pBluescript SK+ between the BamHI/EcoRI sites. The modified polylinker has the following sequence: 5'GAATTCGGCCATTACGGCC(G)n-- insert-- GGCCGCCTCGGCCCACGGATCC3'where n=3-4 G nucleotides. WARNING: the library contains a small percentage of cDNAs derived from the human host cells. Library materials provided by David Sibley, Washington University.

text: Tachyzoite

text: ElectroTen Blue cells (Stratagene)

text: Toxoplasma gondii

text: Tg CAST Tachyzoite cDNA Library

text: plasmid

text: Modified pBluescript (pBS SK+)


text: Centro Biologia Molecular CSIC - UAM

text: live

text: Generic

text: Human mRNAs differentially expressed between human choriocarcinoma cell lines (JEG-3, JAR and BeWo) and normal placental term cells

text: Homo sapiens

text: female

text: placenta

text: Human placenta differentially expressed versus choriocarcinoma cell lines

text: pGEM-T easy


text: Instituto Tecnologico de Celaya-INIFAP

text: live

text: Generic

text: PHV-infected

text: Capsicum chinense

text: Capsicum chinense BG-3821


text: University of Florida

text: live

text: Generic

text: anamorph: Beauveria bassiana; The mRNA was extracted from in vivo fungal cells that were actively growing inside the insect host.

text: in vivo

text: Beauveria bassiana

text: Bb in vivo library

text: plasmid


text: live

text: Generic

text: Cloned unidirectionally. Poly(A)+ RNA was concentrated and purified using Dynabeads (Dynal) and mRNA eluted for first strand synthesis. First strand cDNA was created using MMLV RT (Powerscript, Clontech) and primed with oligo(dT) with XhoI site and 5'SMART 'anchor' added using chimeric DNA-RNA oligo. 12 PCR cycles were done using first strand and primers specific to SMART oligo and 3' end. Double stranded cDNA was digested using XhoI/NotI, fractioned on Chroma-spin 400 columns (Clontech) and ligated to digested pGEM-11zf(+) plasmid. Chemically competent DH10B cells were used as host cells. Library materials provided by Dr. David Bird of North Carolina State University. Library construction by Jeff Rousch. See www.nematode.net for additional project information.

text: Meloidogyne javanica

text: XhoI

text: NotI

text: whole organism

text: Meloidogyne javanica J2 SMART pGEM

text: plasmid (ampicillin resistant)

text: plasmid (ampicillin resistant)


text: Texas Tech University Health Science Center

text: live

text: Generic

text: adult

text: Mesocricetus auratus

text: male

text: Hamster adult testis, seminal vesicle and prostate (pooled)


text: live

text: Generic

text: Meloidogyne arenaria

text: XhoI

text: NotI

text: whole organism

text: Meloidogyne arenaria J2 SMART pGEM

text: plasmid (ampicillin resistant)

text: plasmid (ampicillin resistant)


text: live

text: Generic

text: Homo sapiens

text: testis

text: human testis quick-clone cDNA library


text: Universidad del Quindio

text: live

text: Generic

text: Toxoplasma gondii

text: RH tachyzoite cDNA library


text: live

text: Generic

text: adult

text: Meloidogyne hapla

text: XhoI

text: NotI

text: whole organism

text: Meloidogyne hapla female SMART pGEM

text: plasmid (ampicillin resistant)

text: plasmid (ampicillin resistant)


text: ARC Seibersdorf Research GesmbH

text: live

text: Generic

text: Quercus petraea (Matt.) Liebl.; Smart cDNA library construction kit; Clontech: PT3000-1

text: Lambda

text: Quercus petraea

text: Sfi A

text: Sfi B

text: callus

text: cDNA library of osmotically stressed oak cell culture

text: Phage vector


text: CSIRO

text: live

text: Generic

text: Cabernet Sauvignon

characteristic: cultivar

text: A cDNA library from preveraison fruit, 1-2 weeks post anthesis, at stage 28 of the modified E-L system. Tissue collected from field grown plants. A description of the modified E-L system can be found in the paper by B. G. Coombe 'Adoption of a system for identifying grapevine growth stages' (1995) Aust. J. Grape and Wine Res. 1: 104-110.

text: 28 - modified E-L system

text: Fruit

characteristic: organ

text: Vitis vinifera

text: Hermaphrodite

text: CabSau Berry Fruit Set Stage 28 (PREu0028)

text: Plasmid

text: pZL


text: Chiba Cancer Center Research Institute

text: live

text: Generic

characteristic: NCBI submission package

text: Homo sapiens

characteristic: organism

text: neuroblastoma

text: human favorable neuroblastoma cDNA


text: Chiba Cancer Center Research Institute

text: live

text: Generic

text: Homo sapiens

text: neuroblastoma

text: human unfavorable neuroblastoma cDNA


text: Institut Universitaire Europeen de la Mer, Universite de Bretagne Occidentale

text: live

text: Generic

text: subtracted Libraries from flounder exposed to pesticides (Atrazine-Diuron-Isoproturon)

text: juveniles

characteristic: dev-stage

text: Platichthys flesus

text: gill, muscle and liver


text: live

text: Generic

text: subtracted Libraries from flounder exposed to Glyphosate pesticide

text: juveniles

text: Platichthys flesus

text: gill, muscle and liver


text: National Yeongnam Agricultural Experiment Station

text: live

text: Generic

text: Jeonnamjaerae

text: Hordeum vulgare subsp. vulgare

text: cold acclimated leaves

text: Hordeum vulgare subsp. vulgare cold acclimated leaves


text: Washington University School of Medicine

text: live

text: Generic

text: 1st strand of cDNA was synthesized with reverse transcriptase and oligo(dT) beads, then cDNA was amplified by PCR using modified SMART primers. The final cDNA was cloned in pAMP1 vector in annealing reaction with Uracil DNA Glycosylase (UDG).

text: Gallus gallus

text: Reiterative Chick Utricle Subtraction

text: bacterial


text: Henan Normal University

text: live

text: Generic

text: Rattus norvegicus

text: liver

text: Liver regeneration after partial hepatectomy


text: The University of Georgia, Department of Plant Biology

text: live

text: Generic

text: The library was prepared from polyA+ RNA from loblolly pine (Pinus taeda) roots recovering from drought. Water was withheld from ramet clones until predawn needle water potential reached -1.75 MPa. Plants were well watered on day 7 and allowed to recover for 2 days, at which time roots were harvested for RNA isolation. Double-stranded cDNA was cloned unidirectionally into pSL1180. Inserts excised with EcoRI (5' end) and XhoI (3' end).

text: DH10B-T1 phage-resistant E. coli

text: Pinus taeda

text: EcoRI

text: XhoI

text: CCLONES

characteristic: strain

text: Loblolly pine roots recovering from drought DR1


characteristic: INSDC first public

text: live

text: Generic

text: DH10B-T1 phage-resistant E. coli

text: Pinus taeda

text: EcoRI

text: XhoI

text: CCLONES

text: Loblolly pine roots recovering from drought DR2


text: live

text: Generic

text: DH10B-T1 phage-resistant E. coli

text: Pinus taeda

text: EcoRI

text: XhoI

text: CCLONES

text: Loblolly pine roots recovering from drought DR3


text: live

text: Generic

text: The library was prepared from polyA+ RNA from drought-stressed loblolly pine (Pinus taeda) roots. Water was withheld from ramet clones until predawn needle water potential reached -1.75 MPa. On day 7 roots were harvested for RNA isolation. Double-stranded cDNA was cloned unidirectionally into pSL1180. Inserts excised with EcoRI (5' end) and XhoI (3' end).

text: DH10B-T1 phage-resistant E. coli

text: Pinus taeda

text: EcoRI

text: XhoI

text: CCLONES

text: Drought-stressed loblolly pine roots DS2


text: live

text: Generic

text: DH10B-T1 phage-resistant E. coli

text: Pinus taeda

text: EcoRI

text: XhoI

text: CCLONES

text: Drought-stressed loblolly pine roots DS3


text: live

text: Generic

text: The library was prepared from polyA+ RNA from loblolly pine (Pinus taeda) roots watered to pot capacity every other day. Pre-dawn water potential remained -0.3 MPa +/-0.1. Roots were harvested for RNA isolation. Double-stranded cDNA was cloned unidirectionally into pSL1180. Inserts excised with EcoRI (5' end) and XhoI (3' end).

text: DH10B-T1 phage-resistant E. coli

text: Pinus taeda

text: EcoRI

text: XhoI

text: CCLONES

text: Well-watered loblolly pine roots WW1


text: USDA-ARS, Washington State University

text: live

text: Generic

text: whole roots

characteristic: cell type

text: A cDNA library was constructed from root tissues inoculated with race 1 of Fusarium oxysporum f.sp. ciceri

text: two weeks after germination

text: Cicer arietinum

text: roots

characteristic: tissue-type

text: chickpea root cDNA


characteristic: External Id

text: China Medical University

text: live

text: Generic

text: The sequence was differentially expressed genes between lung adenocarcinoma and its normal tissue which was got by direct cloning of cell differential expression genes. This was identified by cDNA microarray.

text: Homo sapiens

text: Lung adenocarcinoma

text: Human lung adenocarcinoma cDNA library

characteristic: title

text: pGEM-T Easy


text: University of Edinburgh

text: live

text: Generic

text: The library was prepared using protocols given by the supplier (Stratagene).

characteristic: description

text: Late Cocoon

text: Lumbricus rubellus

text: Whole worm

text: Lumbricus rubellus Late Cocoon Library 1

text: pBluescript II SK+


text: Purdue University

characteristic: INSDC center name

text: live

text: Generic

text: Partial sequence of RT region of copia-like elements amplified using 5'-CARATGGAYGTNAARAC-3'and 5'-CATRTCRTCNACRTA-3'primers. PCR product was cloned in PCR4.0 TOPO vector and sequenced

text: Sorghum bicolor

text: nine-day old leaves

text: Sorghum bicolor leaves

text: PCR4.0 TOPO


text: Purdue University

characteristic: INSDC last update

text: live

text: Generic

text: Sorghum bicolor

text: Protoplasts isolated from three-week old embryogenic callus

text: Sorghum bicolor protoplasts

text: PCR4.0 TOPO


text: Purdue University

text: live

characteristic: INSDC status

text: Generic

text: Sorghum bicolor

text: Protoplasts isolated from three-week old embryogenic callus and nine-day old leaves

text: Sorghum bicolor protoplasts and leaves

text: PCR4.0 TOPO


text: USDA, ARS, U. S. Horticultural Research Laboratory

text: live

text: Generic

characteristic: NCBI submission model

text: Valencia

text: Citrus sinensis (L.) Osbeck; Standard library construction protocols from Stratagene cDNA synthesis kit (cat.# 200401-5) and Uni-ZAP XR vector kit (cat.# 237211) were followed using poly(A) RNA.

text: mixed field population

characteristic: isolate

text: Citrus sinensis

text: EcoRI

text: XhoI

text: Flush leaves and stems

text: Citrus sinensis: Insect-damaged immature leaves/stems from field collected Valencia orange

text: Phagemid

text: pBluescript SK(-)


text: live

text: Generic

text: Flying Dragon

characteristic: cultivar

text: Standard library construction protocols from Stratagene cDNA synthesis kit (cat.# 200401-5) and Uni-ZAP XR vector kit (cat.# 237211) were followed using poly(A) RNA

text: Citrus trifoliata

text: EcoRI

text: XhoI

text: Immature ovaries

text: Immature Ovaries from field collected Flying Dragon Trifoliate Orange

text: Phagemid

text: pBluescript SK(-)

characteristic: vector


text: Washington University School of Medicine

text: live

text: Generic

characteristic: NCBI submission package

text: 1st strand primed with an oligo (dT) primer: double stranded cDNA was cloned into the EcoRI and BamHI sites of the pBluescript SK+ vector. Primary library; non-amplified.

characteristic: lab-host

text: Anolis sagrei

text: EcoRI

text: BamHI

text: Anolis sagrei limb bud 2

text: Phagemid

text: Bluescript SK+


text: The University of Georgia, Department of Plant Biology

text: live

text: Generic

text: The library was prepared from polyA+ RNA from BTx623 seedlings grown hydroponically in the absence of added phosphorous. At 14 days of age, roots and shoots were harvested and stored at -80 C until RNA was isolated. Double-stranded cDNA was cloned unidirectionally into different DraIII sites of the pME18S-FL3 vector (5-prime DraIII site is CACTGTGTG, 3-prime DraIII site is CACCATGTG). XhoI excises the cDNA insert.

text: DH10B-T1 phage-resistant E. coli

text: Sorghum bicolor

characteristic: organism

text: XhoI

text: XhoI

text: Phosphorous-deficient seedlings

text: Plasmid


text: live

text: Generic

text: The library was prepared from polyA+ RNA from BTx623 seedlings grown hydroponically in the absence of added nitrogen. At 14 days of age, roots and shoots were harvested and stored at -80 C until RNA was isolated. Double-stranded cDNA was cloned unidirectionally into different DraIII sites of the pME18S-FL3 vector (5-prime DraIII site is CACTGTGTG, 3-prime DraIII site is CACCATGTG). XhoI excises the cDNA insert.

text: DH10B-T1 phage-resistant E. coli

text: Sorghum bicolor

text: XhoI

text: XhoI

text: Nitrogen-deficient seedlings

text: Plasmid


text: National Cancer Institute / NIH

text: live

text: Generic

text: Library is oligo-dT primed and directionally cloned (EcoRV site is destroyed upon cloning). Average insert size 2.1 kb. Library was constructed by Invitrogen and donated by J. Lupski, M.D./Ph.D. (Baylor College of Medicine).

text: DH10B (T1 phage-resistant)

text: Homo sapiens

text: EcoRV (destroyed)

text: NotI

text: Peripheral Nervous system

text: plasmid (ampicillin-resistant)


text: USDA-ARS and University of Wisconsin

text: live

text: Generic

text: Red Creole(bulbs), unknown(callus), Ebano & Texas Legend(roots)

text: Equal molar amounts of mRNA from callus, roots, and young bulbs were combined to synthesize the library. Normalization to enrich for low-copy transcripts was performed by proprietary techniques of Invitrogen.

text: Allium cepa

text: EcoRV (5')

text: NotI (3')

text: Callus, roots, and young bulbs

text: normalized cDNA library of onion

text: plasmid

text: pCMVSport6.1-ccdb (Invitrogen)


text: Purdue University

text: live

text: Generic

text: Fungus was grown on cracked corn. RNA was isolated using phenol LiCl method. PolyA RNA was obtained with Oligotex mRNA spin columns (Qiagen). Subtracted from fcc1 mutant RNA with PCR select cDNA subtraction kit (Clontech), amplified, cloned into pGEM-TEZ and transformed to E. coli DH5 alpha cells.

text: Fusarium verticillioides

text: EcoRI

text: EcoRI

characteristic: strain

text: Fusarium verticillioides wild type subtraction library

text: plasmid

text: PGem-T-Easy