text: DOE Joint Genome Institute

text: live

text: Generic

text: Significant accumulation of itaconate in the media

text: Aspergillus terreus ATCC 32359

text: SalI

text: NotI

text: ATCC 32359

text: mycelia

text: CFBU Aspergillus terreus itaconate-production-phase (H)


status: PUBLIC

characteristic: External Id

text: DOE Joint Genome Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 0.6-2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed and sequencing performed at the DOE Joint Genome Institute (Walnut Creek, CA).

characteristic: description

tag: attribute

characteristic: dev-stage

characteristic: lab-host

text: Aspergillus terreus ATCC 32359

characteristic: organism

text: SalI

text: NotI

text: ATCC 32359

characteristic: strain

text: mycelia

characteristic: tissue-type

text: CFBW Aspergillus terreus itaconate-production-phase (L)

characteristic: title

characteristic: vector


text: Seoul National University

text: live

text: Generic

text: Rice plants (Oryza sativa L. cv. Hwacheong) at the 3rd to 4th leaf stages were spray-inoculated with 2 X 105 spores/ml. After keeping in a dew chamber for 24 h at 25 oC, the inoculated plants were transferred to the greenhouse to let the lesions develop. Rice leaves on which typical susceptible type lesions spread through the entire leaves were cut and immediately frozen in liquid nitrogen daily up to 10 days after inoculation (in planta sample). Total RNA was isolated from the frozen plant tissues through phenol/chloroform extraction, followed by lithium chloride precipitation. Poly A(+) RNA was purified using the PolyATrack mRNA isolation system (Promega, USA) and used for construction of two libraries. A subtracted cDNA library was constructed by suppression subtractive hybridization using PCR-selectTM cDNA subtraction kit (Clontech, Palo Alto, CA, USA) following the manufacturer's instructions. cDNA obtained from M. oryzae infected rice leaves was used as a 'tester' and cDNA from uninfected healthy leaves was used as a 'driver'. Subtracted PCR products were cloned into pCR4 blunt-TOPO vector (Invitrogen) and transformed into E. coli strain TOP10 (Invitrogen) with blue-white selection. White colonies were grown in LB plates amended with ampicillin and used for plasmid preparation.

text: Pyricularia oryzae

text: Rice leaves with expanding blast lesions subtracted with uninfected leaves

text: Infection EST_SSH of Magnaporthe oryzae

text: pCR4blunt-TOPO vector


text: Seoul National University

text: live

text: Generic

text: Rice plants (Oryza sativa L. cv. Hwacheong) at the 3rd to 4th leaf stages were spray-inoculated with 2 X 105 spores/ml. After keeping in a dew chamber for 24 h at 25 oC, the inoculated plants were transferred to the greenhouse to let the lesions develop. Rice leaves on which typical susceptible type lesions spread through the entire leaves were cut and immediately frozen in liquid nitrogen daily up to 10 days after inoculation (in planta sample). Total RNA was isolated from the frozen plant tissues through phenol/chloroform extraction, followed by lithium chloride precipitation. Poly A(+) RNA was purified using the PolyATrack mRNA isolation system (Promega, USA) and used for construction of two libraries. cDNA was synthesized using the lamda ZAP cDNA synthesis kit and cDNAs larger than 0.5 kb after gel filtration were used to construct the cDNA library (infection library: IL) using the ZAP Express cDNA Gigapack III Gold Cloning kit (Stratagene, La Jolla, CA, USA). Mass in vivo excision was conducted to rescue phagemid clones. Individual colonies were grown in LB medium amended with 100 ug/ml ampicillin in 96 well plates and used for plasmid preparation.

text: Pyricularia oryzae

text: EcoRI

text: XhoI

text: Rice leaves with expanding blast lesions

text: Infection EST of Magnaporthe oryzae

text: ZAP Express


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected 0.5-2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed at DOE Joint Genome Institute (Walnut Creek, CA).

text: Thermothelomyces thermophilus ATCC 42464

text: SalI

text: NotI

text: ATCC 42464

text: mycelia

text: CCWO Myceliophthora thermophila ATCC 42464 1% cellulose and 1% pectin pooled culture (H)


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected >2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed at DOE Joint Genome Institute (Walnut Creek, CA).

text: SalI

text: NotI

text: ATCC 42464

text: mycelia

text: CCWP Myceliophthora thermophila ATCC 42464 1% cellulose and 1% pectin pooled culture (L)


text: China Agricultural University

text: live

text: Generic

text: White Leghorn chicken embryo

text: embryonic day 2

text: Gallus gallus

text: White Leghorn

text: chicken embryonic day 2


text: Northwest A&F University

text: live

text: Generic

text: alabastrum

text: alabastrum

characteristic: organ

text: Brassica rapa subsp. pekinensis

text: alabastrum

text: Chinese cabbage cytoplasmic male sterility


text: Faculty of Horticulture, Uttra Banga Krishi Viswavidyalaya (North Bengal Agricultural University)

text: live

text: Generic

text: TV-23 and S3A/3

characteristic: cultivar

text: Drought stressed root SSH cDNA library of Camellia sinensis cDNA. A SSH library of root of the tea plants after 21 days of drought stress treatment was made according to the instructions given in Clontech Manual on PCR Select cDNA Subtraction Kit (Cat. No. 637401; Clontech Lab Inc, USA).

text: Around 21 days water stress

text: Root

text: Camellia sinensis var. assamica

text: TEASSHUBKV

text: pGEM-T


characteristic: External Id

text: College of Agronomy Agricultural University of Hebei

text: live

text: Generic

text: leaf infected by aphid

characteristic: cell type

text: 8 leaf

text: Sorghum bicolor

text: leaf

text: Sorghum HN16 leaf infected by aphid


text: State University of Campinas - UNICAMP

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: Library was prepared from total RNA based on the Gateway Technology (CloneMiner cDNA Library Construction Kit. Invitrogen 182 49-029).

text: Mixed stages

text: Seed

text: Jatropha curcas

text: Developing endosperm

text: Jatropha curcas L. developing seeds (mixed stages)

text: Plasmid

text: pDONR 222


text: State University of Campinas - UNICAMP

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Mixed stages

text: Seed

text: Jatropha curcas

text: Germinating endosperm

text: Jatropha curcas L. germinating seeds (mixed stages)

text: Plasmid

text: pDONR 222


text: DOE Joint Genome Institute

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: embryo

text: Tetranychus urticae

text: SalI

text: NotI

text: London

text: CCIN Tetranychus urticae London embryo (L)


text: DOE Joint Genome Institute

text: live

text: Generic

characteristic: description

text: embryo

characteristic: dev-stage

text: Tetranychus urticae

text: SalI

text: NotI

text: London

text: CCIO Tetranychus urticae London embryo (H)


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT)-primed cDNA and cloned into vector pDNR-LIB (Clontech Laboratories, Inc., Mountain View, CA) using the SMART system (BD Biosciences, San Jose, CA). To generate the first strand, polyA RNA was primed with an oligo(dT) primer (5'-ATTCTAGAGGCCGAGGCGGCCGACATG(T)30 VN-3', where V = A, G, or C). The second strand was synthesized by PCR using primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' followed by digestion of the SfiI sites. cDNA was size selected then ligated into pDNR-LIB. Library is normalized. Library was created and sequencing done by the DOE Joint Genome Institute (Walnut Creek, CA).

text: larvae

characteristic: lab-host

text: Tetranychus urticae

characteristic: organism

text: SfiIA

text: SfiIB

text: London

text: CCZA Tetranychus urticae London larvae normalized

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: adult

text: Tetranychus urticae

text: SfiIA

text: SfiIB

text: London

characteristic: strain

text: CCZB Tetranychus urticae London adult normalized

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: Pooled RNA from 5 different treatments (optimal growth, supercooling at -4oC, salt stress at 60PSU, iron and silicate limitation). The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed and sequencing performed at the DOE Joint Genome Institute (Walnut Creek, CA).

text: Fragilariopsis cylindrus

text: SalI

text: NotI

text: CCMP 1102

text: CCUX Fragilariopsis cylindrus pooled RNA

characteristic: title


text: DOE Joint Genome Institute

text: live

text: Generic

text: The SMART-PCR amplification method (Clontech, Mountain View, CA) was used for cDNA synthesis from 1 ug of total RNA followed by directional cloning in SfiI digested Triplex2 vector. Inserts were size selected with Chromaspin 1000 columns (Clonetech). Phage were converted to plasmid subclones (pTriplEx) by cre-lox excision in E. coli strain BM25.8. cDNAs were sequenced from the 5' end with the 5'Triplex sequencing primer (Tpx) and the 3' end with the T7 primer (T7). Library constructed in the laboratory of P. Lammer (New Mexico State University) and sequencing performed by the DOE Joint Genome Institute (Walnut Creek, CA).

text: Rhizophagus intraradices

text: SfiIA

text: SfiIB

text: DAOM 181602

characteristic: tissue-type

text: CACE Glomus intraradices extraradical mycelium prior to sporulation

text: pTriplEx

characteristic: vector


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT)-primed cDNA and cloned into vector pDNR-LIB (Clontech Laboratories, Inc., Mountain View, CA) using the SMART system (BD Biosciences, San Jose, CA). To generate the first strand, polyA RNA was primed with an oligo(dT) primer (5'-ATTCTAGAGGCCGAGGCGGCCGACATG(T)30 VN-3', where V = A, G, or C). The second strand was synthesized by PCR using primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' followed by digestion of the SfiI sites. cDNA was size selected from 2-8kb using 1.1% agarose gel electrophoresis, then ligated into pDNR-LIB. This normalized library was created and sequencing performed by the DOE Joint Genome Institute (Walnut Creek, CA).

text: Rhizophagus intraradices

text: SfiIA

text: SfiIB

text: DAOM 181602

text: CCHU Glomus intraradices germinated spores on water agar 3 days

text: pDNR-LIB


text: Meiji Pharmaceutical University

text: live

text: Generic

text: Rhabdophis tigrinus tigrinus

text: venom gland

text: Rhabdophis tigrinus tigrinus Duvernoy's glands cDNA library


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT)-primed cDNA and cloned into vector pDNR-LIB (Clontech Laboratories, Inc., Mountain View, CA) using the SMART system (BD Biosciences, San Jose, CA). To generate the first strand, polyA RNA was primed with an oligo(dT) primer (5'-ATTCTAGAGGCCGAGGCGGCCGACATG(T)30 VN-3', where V = A, G, or C). The second strand was synthesized by PCR using primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' followed by digestion of the SfiI sites. cDNA was ligated into pDNR-LIB. Library was created in the laboratory of D. Jackson at the University of Queensland and sequencing done by the DOE Joint Genome Institute (Walnut Creek, CA).

text: larvae

text: Amphimedon queenslandica

text: SfiI A

text: SfiI B

text: CABF Amphimedon queenslandica released competant larvae

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT)-primed cDNA and cloned into vector pDNR-LIB (Clontech Laboratories, Inc., Mountain View, CA) using the SMART system (BD Biosciences, San Jose, CA). To generate the first strand, polyA RNA was primed with an oligo(dT) primer (5'-ATTCTAGAGGCCGAGGCGGCCGACATG(T)30 VN-3', where V = A, G, or C). The second strand was synthesized by PCR using primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' followed by digestion of the SfiI sites. cDNA was size selected >2kb using 1.1% agarose gel electrophoresis, then ligated into pDNR-LIB. Library was created and sequencing done by the DOE Joint Genome Institute (Walnut Creek, CA).

text: 1 day old larvae

text: DH10B T1 resistant

text: Amphimedon queenslandica

text: SfiI A

text: SfiI B

text: whole

text: CAYH Amphimedon queenslandica competent larva (one day old)

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT)-primed cDNA and cloned into vector pDNR-LIB (Clontech Laboratories, Inc., Mountain View, CA) using the SMART system (BD Biosciences, San Jose, CA). To generate the first strand, polyA RNA was primed with an oligo(dT) primer (5'-ATTCTAGAGGCCGAGGCGGCCGACATG(T)30 VN-3', where V = A, G, or C). The second strand was synthesized by PCR using primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' followed by digestion of the SfiI sites. cDNA was size selected from 1.2-2kb using 1.1% agarose gel electrophoresis, then ligated into pDNR-LIB. Library was created and sequencing done by the DOE Joint Genome Institute (Walnut Creek, CA).

text: 1 day old larvae

text: DH10B T1 resistant

text: Amphimedon queenslandica

text: SfiI A

text: SfiI B

text: whole

text: CAYI Amphimedon queenslandica competent larva (one day old) (L)

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT)-primed cDNA and cloned into vector pDNR-LIB (Clontech Laboratories, Inc., Mountain View, CA) using the SMART system (BD Biosciences, San Jose, CA). To generate the first strand, polyA RNA was primed with an oligo(dT) primer (5'-ATTCTAGAGGCCGAGGCGGCCGACATG(T)30 VN-3', where V = A, G, or C). The second strand was synthesized by PCR using primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' followed by digestion of the SfiI sites. cDNA was size selected using 1.1% agarose gel electrophoresis, then ligated into pDNR-LIB. Library is normalized. Library was created and sequencing performed by the DOE Joint Genome Institute (Walnut Creek, CA).

text: pooled larvae and adult

text: DH10B T1-resistant

text: Acropora palmata

text: CCHX Acropora palmata heat/dark/disease-stressed adult + infected larvae

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected >2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed at DOE Joint Genome Institute (Walnut Creek, CA).

text: DH10B T1R

text: Coccomyxa subellipsoidea C-169

text: SalI

text: NotI

text: CBOZ Coccomyxa sp. C-169 8kb


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 1.2-2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed at DOE Joint Genome Institute (Walnut Creek, CA).

text: DH10B T1R

text: Coccomyxa subellipsoidea C-169

text: SalI

text: NotI

text: CBPA Coccomyxa sp. C-169 2kb


text: DOE Joint Genome Institute

text: live

text: Generic

text: pooled larvae and adult

text: DH10B T1-resistant

text: Orbicella faveolata

text: CCHW Montastraea faveolata heat/dark/disease-stressed adult + 6 day old larvae

text: pDNR-LIB


text: University of Florida

text: live

text: Generic

text: Florunner

characteristic: cultivar

text: Root

characteristic: organ

text: Arachis hypogaea

text: Root infected with Meloidogyne arenaria

text: Florunner-specific SSH library


characteristic: External Id

text: University of Florida

text: live

text: Generic

text: NemaTAM

text: Root

text: Arachis hypogaea

text: Root infected with Meloidogyne arenaria

text: NemaTAM-specific SSH library


text: Bharathiar University, Department of Biotechnology

characteristic: INSDC center name

text: live

text: Generic

text: Kuroda

text: A substraction cDNA library of Daucus carota was constructed after giving cold stress to identify potential cold tolerant genes

text: Vegetative

text: Daucus carota

text: Hermaphrodite

characteristic: sex

text: Leaf

text: Daucus carota cold responsive cDNA substraction Library


text: Institute of Arctic Biology

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Quadriceps

text: Ursus americanus

text: SfiI

text: SfiI

text: male

text: skeleton muscle

text: black bear skeleton muscle library

text: Plasmid

text: pDNR-LIB


text: Institute of Arctic Biology

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Ursus americanus

text: SfiI

text: SfiI

text: male

text: testis

text: black bear testis library

text: Plasmid

text: pDNR-LIB


text: Institute of Arctic Biology

text: live

text: Generic

characteristic: NCBI submission package

text: Ursus americanus

text: SfiI

text: SfiI

text: male

text: heart

text: black bear heart library

text: Plasmid

text: pDNR-LIB


text: Institute of Arctic Biology

text: live

text: Generic

text: Ursus americanus

text: SfiI

text: SfiI

text: male

text: liver

text: black bear liver library

text: Plasmid

text: pDNR-LIB


text: Institute of Arctic Biology

text: live

text: Generic

text: Ursus americanus

text: SfiI

text: SfiI

text: male

text: brain

text: black bear brain library

text: Plasmid

text: pDNR-LIB


text: Jinan University

text: live

text: Generic

text: obtained by RT-PCR

characteristic: description

text: Human betaherpesvirus 5

text: HCMV Towne UL49


text: South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences

text: live

text: Generic

text: Mature with shell length 50 5mm

characteristic: dev-stage

characteristic: lab-host

text: Whole Body

text: Haliotis diversicolor

text: SfiI

text: SfiI

text: Female, Male

text: Haliotis diversicolor SSR-EST Partial Sequences

text: Plasmid

text: pDNR-LIB


text: Federal University of Rio de Janeiro

text: live

text: Generic

text: The library was generated from mRNA isolated from different individuals. The cDNA was synthetized using the SMART approach and normalized by the DSN method.

text: adult

text: Escherichia coli

text: Digestive gland

text: Crassostrea rhizophorae

characteristic: organism

text: SfiIA

text: SfiIB

text: Mangrove oyster digestive gland normalized library

text: plasmid

text: pDNR-LIB


text: Federal University of Rio de Janeiro

text: live

text: Generic

text: The library was generated from total RNA isolated from a haemolymph pool. The cDNA was synthetized using the SMART approach and normalized by the DSN method.

text: adult

text: Escherichia coli

text: Crassostrea rhizophorae

text: SfiIA

text: SfiIB

text: Haemocytes

text: Mangrove oyster Haemocytes normalized library

characteristic: title

text: plasmid

text: pDNR-LIB


text: Federal University of Rio de Janeiro

text: live

text: Generic

text: adult

text: Escherichia coli

text: Digestive gland

text: Nodipecten nodosus

text: SfiIA

text: SfiIB

text: N. nodosus digestive gland normalized library

text: plasmid

text: pDNR-LIB


text: Heilongjiang Bayi Agricultural University

text: live

text: Generic

text: The experiment was conducted to constructed the full-length cDNA library of the pituitary in the Zi geese (Anser anser) by SMART (Switching Mechanism at 5' end of RNA Transcript) using lambdaTriplEx2 as vector. Total RNA was extracted from fresh female Zi Geese at month 6.

text: month 6

text: Anser anser

text: female

text: pituitary

characteristic: tissue-type

text: Zi Geese pituitary month 6 Library

text: Phage


text: Nanjing Agricultural University

text: live

text: Generic

text: anthesis

text: flower bud

text: Brassica rapa subsp. chinensis

text: Ogura CMS line and its maintainer of non-heading Chinese cabbage


text: Marine Biosciences Graduate School of Fisheries Sciences, Hokkaido University

text: live

text: Generic

text: Planiliza haematocheilus

text: Chelon haematocheilus vitellogenin cDNA library


text: Wellcome Trust Sanger Institute

text: live

text: Generic

characteristic: isolate

text: Plasmodium falciparum 3D7

characteristic: strain


text: University of Northern Colorado

text: live

text: Generic

text: young diesel tree grown in plant incubator

text: Copaifera officinalis

text: leaves and stem

text: diesel tree sapling


text: Hirosaki University

text: live

text: Generic

text: subtracted cDNA highly containing vascular bundle specific genes in Brassica napus

text: Brassica napus

text: vascular bundle


text: National Research Institute of Aquaculture

text: live

text: Generic

text: Subtractive hybridization was performed with the PCR-Selectct DNA Subtraction Kit (Clontech), according to the manufacture's protocol. Total RNA from PBL of E. tarda infected fish and PBS injected fish was used to make tester and driver cDNAs, respectively. Two subtractions were performed: the original intended subtraction (forward subtraction), and a reverse subtraction in which tester serves as the driver and the driver as tester.

text: adult

text: Paralichthys olivaceus

text: mixed

text: peripheral blood leucocyte

text: Paralichthys olivaceus peripheral blood leucocyte adult


text: Nagoya University

text: live

text: Generic

text: immature

text: Pyrus pyrifolia

text: fruit

text: Japanese pear 'Housui' cDNA library immature fruit


text: Nagoya University

text: live

text: Generic

text: leaf bud

text: Pyrus pyrifolia

text: leaf

text: Japanese pear 'Housui' cDNA library leaf bud


text: Nagoya University

text: live

text: Generic

text: mature

text: Pyrus pyrifolia

text: leaf

text: Japanese pear 'Housui' cDNA library mature leaf


text: Nagoya University

text: live

text: Generic

text: flower before opening

text: Pyrus pyrifolia

text: flower

text: Japanese pear 'Housui' cDNA library flower before opening


text: Nagoya University

text: live

text: Generic

text: over-ripened

text: Pyrus pyrifolia

text: fruit

text: Japanese pear 'Housui' cDNA library over-ripened fruit


text: National Institute of Fruit Tree Science

text: live

text: Generic

text: mature

text: Pyrus pyrifolia

text: fruit

text: Japanese pear cDNA library mature fruit


text: live

text: Generic

text: flower at full bloom

text: Pyrus pyrifolia

text: flower

text: Japanese pear 'Housui' cDNA library flower at full bloom


text: live

text: Generic

text: mature

text: Pyrus pyrifolia

text: fruit

text: Japanese pear 'Housui' cDNA library mature fruit


text: Nagoya University

text: live

text: Generic

text: flower bud

text: Pyrus pyrifolia

text: flower

text: Japanese pear 'Housui' cDNA library flower bud


text: Zhejiang University, College of Agriculture and Biotechnology

text: live

text: Generic

text: Pupa

text: Fat body

characteristic: organ

text: Pieris rapae

text: Unsexed

text: Fat body

text: Forward subtractive library of fat body from pupal white cabbage butterfly

text: Plasmid

text: pGEM T-Easy Vector

characteristic: vector


characteristic: External Id

text: National Institute of Vegetable and Tea Science

characteristic: INSDC center name

text: live

text: Generic

text: Camellia sinensis

text: root

text: Camellia sinensis root


text: Inje University College of Medicine

characteristic: INSDC first public

text: live

text: Generic

text: mast cell

characteristic: cell type

characteristic: cell-line

text: Homo sapiens

text: full-length enriched Human Mast Cell cDNA library


text: Inje University College of Medicine

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: mast cell

text: Homo sapiens

text: full-length enriched Human Mast Cell cDNA library (PAMP; Pathogen Associated Molecular Pattern treated)


text: Inje University College of Medicine

text: live

text: Generic

characteristic: NCBI submission package

text: adult

characteristic: dev-stage

text: Nesiohelix samarangae

text: hermaphrodite

characteristic: sex

text: whole body

text: full-length enriched Nesiohelix samarangae cDNA library


text: Inje University College of Medicine

text: live

text: Generic

text: adult

text: Nesiohelix samarangae

characteristic: organism

text: hermaphrodite

text: whole body

text: full-length enriched Nesiohelix samarangae cDNA library (Laminarin as a PAMP; Pathogen Associated Molecular Pattern treated)

characteristic: title


text: Vasantdada Sugar Institute

text: live

text: Generic

text: Total mRNA was extracted from healthy and SCGS infected sugarcane leaf tissue profiled by RAPD primers. Sugarcane cultivar Co 86032 at tillering stage.

characteristic: description

text: E.coli DH5alpha

characteristic: lab-host

text: Candidatus Phytoplasma sacchari

text: Leaf

characteristic: tissue-type

text: cDNA-RAPD fragment of SCGS Phytoplasma

text: InsTA PCR cloning vector (Fermentas)


text: Anna University, Chennai

text: live

text: Generic

text: Adult

text: Penaeus monodon

text: Hemocytes

text: EST library from normal Indian tiger shrimp Penaeus monodon hemocytes

text: Plasmid

text: pSPORT 1

characteristic: vector


characteristic: External Id

text: live

text: Generic

text: Pupa

text: Hemocyte

characteristic: organ

text: Pieris rapae

text: Unsexed

text: Hemocyte

text: Forward subtractive library of hemocytes from pupal white cabbage butterfly

text: Plasmid

text: pGEM T-easy


text: MPI for Molecular Genetics

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: The animal was kindly provided by Juergen Markl, Mainz. Tissue was shock-frozen and grinded in liquid nitrogen. Total RNA was extracted as described by Holmes and Bonner (1973) and was further purified using the NucleoSpin RNA II kit (Macherey-Nagel, Dueren, Germany) including a DNase digest.

text: adult

text: Limulus polyphemus

text: male

text: whole animal


text: MPI for Molecular Genetics

text: live

text: Generic

text: country: Germany:Bonn collection_date: Jun-2006 collected_by: Karen Meusemann, Johannes Dambach

text: adult

text: Campodea fragilis

text: whole animal


text: MPI for Molecular Genetics

text: live

text: Generic

text: country: Germany:Bonn collection_date: May-2007 collected_by: Karen Meusemann, Johannes Dambach

text: adult

text: Campodea fragilis

text: whole animal


text: MPI for Molecular Genetics

text: live

text: Generic

text: The animals were collected with the help of scientists from the Istituto di Scienze Marine, Venice (Italy). Animals were shock-frozen and grinded in liquid nitrogen. Total RNA was extracted as described by Holmes and Bonner (1973) and was further purified using the NucleoSpin RNA II kit (Macherey-Nagel, Dueren, Germany) including a DNase digest. Poly(A)+ RNA was enriched from the total RNA using Dynabeads Oligo(dT)25 (Invitrogen, Carlsbad, USA). country: Italy:Venice, Lagoon collection_date: Sep-2006

text: Endeis spinosa

text: whole animal


text: MPI for Molecular Genetics

text: live

text: Generic

text: country: Austria:Carinthia collection_date: Oct-2007 collected_by: Karen Meusemann, Manfred Walzl, Guenther Pass, Johannes Dambach, Daniela Bartel

text: adult

text: Acerentomon franzi

text: whole animal


text: MPI for Molecular Genetics

text: live

text: Generic

text: country: Netherlands:Texel collection_date: Aug-2007 collected_by: Karen Meusemann, Johannes Dambach Sampling location: Bay around the ferry dock of the island Texel, Netherlands.

text: adult

text: Anurida maritima

text: whole animal


text: MPI for Molecular Genetics

text: live

text: Generic

text: The animal was purchased from a local pet shop. The animal was shock-frozen and grinded in liquid nitrogen. Total RNA was extracted as described by Holmes and Bonner (1973) and was further purified using the NucleoSpin RNA II kit (Macherey-Nagel, Dueren, Germany) including a DNase digest.

text: adult

text: Archispirostreptus gigas

text: female

text: whole animal


text: MPI for Molecular Genetics

text: live

text: Generic

text: country: Austria:Vienna collection_date: May-2007 collected_by: Nikolaus U. Szucsich

text: adult

text: Lepismachilis y-signata

text: whole animal


text: MPI for Molecular Genetics

text: live

text: Generic

text: country: Austria:Vienna collection_date: Oct-2006 collected_by: Nikolaus U. Szucsich

text: adult

text: Lepismachilis y-signata

text: whole animal


text: MPI for Molecular Genetics

text: live

text: Generic

text: The animal was kindly provided by Hilke Ruhberg, University of Hamburg. The animal was shock-frozen and grinded in liquid nitrogen. Total RNA was extracted as described by Holmes and Bonner (1973) and was further purified using the NucleoSpin RNA II kit (Macherey-Nagel, Dueren, Germany) including a DNase digest.

text: adult

text: Peripatopsis sedgwicki

text: male

text: whole animal


text: MPI for Molecular Genetics

text: live

text: Generic

text: country: Spain:Ferrol collection_date: 2007 collected_by: J. Cristobo

text: adult

text: Pollicipes pollicipes

text: parts of muscle and other tissues


text: MPI for Molecular Genetics

text: live

text: Generic

text: country: USA; collection_date: 2008

text: adults of different development stages

text: Tigriopus californicus

text: whole animal


text: Madras Veterinary College

text: live

text: Generic

text: country: India

text: Mecistocirrus digitatus

text: Mecistocirrus digitatus library (Raman M)


text: University of Sheffield

text: live

text: Generic

text: adult

text: Chorthippus parallelus parallelus

text: testis

text: Chorthippus parallelus parallelus testis SSH


text: University of Sheffield

text: live

text: Generic

text: adult

text: Chorthippus parallelus erythropus

text: testis

text: Chorthippus parallelus erythropus testis SSH


text: Universite Claude Bernard Lyon 1

text: live

text: Generic

text: environmental_sample ; country: France:South-west France, Cap Ferret ; lat_lon: 44.43 N 1.15 W ; collection_date: 18-Nov-2004 ; collected_by: Bailly J., Marmeisse R.

text: uncultured eukaryote

text: uncultured eukaryote from sandy soil from a Pinus pinaster forest, Cap Ferret


text: Anhui Agricultural University

text: live

text: Generic

characteristic: cell-line

text: Camellia sinensis

text: hermaphrodite

text: callus

text: Callus SSH cDNA library of tea plant (Camellia sinensis) induced by light 2


text: Kunming University of Science and Technology

characteristic: INSDC last update

text: live

text: Generic

text: Yumu No.1

characteristic: cultivar

text: suppression subtractive hybridization library for Medicago sativa under aluminum (Al) stress

text: exposed to Al stress (5 uM) for 15 min,30min, 1h, 2h, 4h, 6h, 10h, 12h, 24h respectively

text: E.coli DH5a

text: root

text: Medicago sativa

text: root

text: SSH library for Medicago sativa under aluminum (Al) stress

text: pGEM-T Easy Vector


text: Indian Institute of Integrative Medicine (CSIR)

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Total RNA extracted from root tissue and subsequently constructed into cDNA library using modified 5' RACE protocol. Clones were screened and the amplified products were sequenced using M-13 primers.

text: Mature plant

text: Withania somnifera

text: Bisexual

text: Root

text: Withania somnifera cv. WS-Y-08 root tissue


text: Henan Agricultural University

text: live

text: Generic

characteristic: NCBI submission package

text: GUSHI-ANAK chicken F2 reference family

text: 12 weeks of age

characteristic: dev-stage

text: Gallus gallus

text: male and female

text: liver

text: chicken breast blister (keel cyst) related genes

text: pUCM-T


text: University of Heilongjiang

text: live

text: Generic

text: mycorrhizal root cell

characteristic: cell type

text: EST obtained through DDRT-PCR. Arbuscular mycorrhizal fungi (Glomus mosseae) and plant Amorpha fruticosa.

text: 14 day after inoculation

text: Amorpha fruticosa/Glomus mosseae mixed EST library

characteristic: organism

text: Amorpha fruticosa/Glomus mosseae arbuscular mycorrhiza Library


text: South African Sugar Association Experiment Station

text: live

text: Generic

text: Saccharum hybrid cultivar NCo 376

characteristic: strain

text: young leaf roll

text: Sugarcane cDNA

characteristic: title


text: live

text: Generic

text: mRNA was prepared from mature stalk material of the commercial sugarcane variety Nco 376. cDNA was synthesized using standard protocols and ligated to an annealed amplification adaptor set. cDNA was then PCR amplified. Amplified cDNA was ligated and cloned into the Eco RI site of the Lambda ZAP II phage vector.

characteristic: description

text: Saccharum hybrid cultivar NCo 376

text: EcoRI

text: variety Nco 376

text: Sugarcane mature stalk

text: Phage

text: Lambda ZAP II


text: University of Georgia

text: live

text: Generic

text: Badila

characteristic: lab-host

text: Saccharum officinarum

text: EcoRI

text: XhoI

text: Germinating lateral buds

characteristic: tissue-type

text: CDSB

text: Plasmid

text: UniZAP XR/pBluescript

characteristic: vector


characteristic: External Id

text: University of Georgia

characteristic: INSDC center name

text: live

text: Generic

text: Saccharum hybrid cultivar H50-7209

text: EcoRI

text: XhoI

text: Cell suspension culture grown under constant light

text: CDSC

text: UniZAP XR/pBluescript


text: University of Georgia

characteristic: INSDC first public

text: live

text: Generic

text: Badila

text: Saccharum officinarum

text: EcoRI

text: XhoI

text: Germinating sett roots

text: CDSR

text: Plasmid

text: UniZAP XR/pBluescript


text: University of Georgia

characteristic: INSDC last update

text: live

text: Generic

text: After floral induction

text: Saccharum hybrid cultivar CP72-2086

text: Leaves

text: pSL

text: Plasmid

text: Uni-ZAP XR /pBluescript


text: University of Georgia

text: live

text: Generic

characteristic: cultivar

text: After floral induction

text: Saccharum hybrid cultivar CP72-2086

text: Stem

text: pSS

text: Plasmid

text: Uni-ZAP XR/pBluescript


text: University of Georgia

text: live

characteristic: INSDC status

text: Generic

text: After floral induction

text: Saccharum hybrid cultivar CP72-2086

text: XhoI

text: Leafroll including apex

text: pSLR

text: Plasmid

text: UniZAP XR/pBluescript


text: Organization for Nucleotide Sequencing and Analysis

text: live

text: Generic

characteristic: NCBI submission model

text: Saccharum hybrid cultivar SP80-3280

text: Saccharum officinarum mRNA


text: live

text: Generic

characteristic: NCBI submission package

text: Saccharum hybrid cultivar SP80-3280

text: Saccharum officinarum mRNA (Nogueira,F.T.S)


text: Commonwealth Scientific and Industrial Research Organisation Division of Plant Industry

text: live

text: Generic

text: mRNA was prepared from the meristem and internodes 1-3 of the sugarcane variety Q117. cDNA was synthesised from a NotI-oligodT primer/adapter using the manufacturers protocols (Life Technologies) and then ligated to a SalI adapter to facilitate directional cloning. The cDNA was cloned into the SalI and NotI sites of the Lambda ZIPLOX phage vector (Life Technologies). Constructed by Chrisopher P.L. Grof.

text: 12 months post-planting

characteristic: dev-stage

text: Meristem, internodes 1-3

characteristic: organ

text: Saccharum hybrid cultivar Q117

text: SalI

text: NotI

text: Immature Sugarcane Stem Lambda ZIPLOX Library (YCS)

text: Phage

text: Lambda ZIPLOX


text: live

text: Generic

text: mRNA was prepared from internodes 6-11 of the sugarcane variety Q117. cDNA was synthesised from a NotI-oligodT primer/adapter using the manufacturers protocols (Life Technologies) and then ligated to a SalI adapter to facilitate directional cloning. The cDNA was cloned into the SalI and NotI sites of the Lambda ZIPLOX phage vector (Life Technologies). Constructed by Rosanne E. Casu.

text: 12 months post-planting

text: internodes 6-11

text: Saccharum hybrid cultivar Q117

characteristic: organism

text: SalI

text: NotI

text: Maturing Sugarcane Stem Lambda ZIPLOX Library (MCS)

text: Phage

text: Lambda ZIPLOX


text: Universidade Estadual de Campinas

text: live

text: Generic

text: An unidirectional cDNA library generated from [seedlings inoculated with Gluconacetobacter diazotroficans]. cDNA was prepared from polyA+ mRNA using SuperScript Plasmid System Kit (Invitrogen). The double-strand cDNAs were fractionated in a sepharose CL-2B 40cm-columns and fragments sizing between 0.8 and 1.5 Kb were directionally cloned into the vector. Details of each source of RNA and library construction can be obtained at http://sucest.lad.ic.unicamp.br/public

text: Saccharum hybrid cultivar SP70-1143

text: SalI

text: NotI


text: Universidade Estadual de Campinas

text: live

text: Generic

text: An unidirectional cDNA library generated from [Apical meristem and tissues surrounding of mature plants]. cDNA was prepared from polyA+ mRNA using SuperScript Plasmid System Kit (Invitrogen). The double-strand cDNAs were fractionated in a sepharose CL-2B 40cm-columns and fragments sizing between 0.8 and 1.5 Kb were directionally cloned into the vector. Details of each source of RNA and library construction can be obtained at http://sucest.lad.ic.unicamp.br/public

text: Saccharum hybrid cultivar SP80-3280

text: SalI

text: NotI


text: Universidade Estadual de Campinas

text: live

text: Generic

text: An unidirectional cDNA library generated from [Apical meristem and tissues surrounding of immature plants]. cDNA was prepared from polyA+ mRNA using SuperScript Plasmid System Kit (Invitrogen). The double-strand cDNAs were fractionated in a sepharose CL-2B 40cm-columns and fragments sizing between 0.8 and 1.5 Kb were directionally cloned into the vector. Details of each source of RNA and library construction can be obtained at http://sucest.lad.ic.unicamp.br/public

text: Saccharum hybrid cultivar SP80-3280

text: SalI

text: NotI


text: Universidade Estadual de Campinas

text: live

text: Generic

text: An unidirectional cDNA library generated from [Pool of sugarcane calli submitted to low (4oC) and high (37 C) temperature stress]. cDNA was prepared from polyA+ mRNA using ZAP - cDNA Synthesis Kit (Stratagene). The double-strand cDNAs were fractionated in a sepharose CL-2B 40cm-columns and fragments sizing between 0.8 and 1.5 Kb were directionally cloned into the vector. Details of each source of RNA and library construction can be obtained at http://sucest.lad.ic.unicamp.br/public

text: XL1Blue MRF'

text: Saccharum hybrid cultivar

text: EcoRI

text: XhoI

text: pBlurScript


text: Universidade Estadual de Campinas

text: live

text: Generic

text: XL1Blue MRF'

text: Pool of sugarcane calli submitted to low (4oC) and high (37 C) temperature stress

text: Saccharum hybrid cultivar

text: EcoRI

text: XhoI

text: pBlurScript


text: Universidade Estadual de Campinas

text: live

text: Generic

text: An unidirectional cDNA library generated from [Pool of sugarcane calli submitted to low (4oC) and high (37 C) temperature stress]. cDNA was prepared from polyA+ mRNA using SuperScript Plasmid System Kit (Invitrogen). The double-strand cDNAs were fractionated in a sepharose CL-2B 40cm-columns and fragments sizing between 0.8 and 1.5 Kb were directionally cloned into the vector. Details of each source of RNA and library construction can be obtained at http://sucest.lad.ic.unicamp.br/public

text: Saccharum hybrid cultivar

text: SalI

text: NotI


text: Universidade Estadual de Campinas

text: live

text: Generic

text: Saccharum hybrid cultivar SP80-3280

text: SalI

text: NotI


text: Universidade Estadual de Campinas

text: live

text: Generic

text: SP83-5077; SP80-185; SP87-396; SP80-3280; SP803280 x SP81-5441

text: An unidirectional cDNA library generated from [Inflorescence at begining of development (1cm-long)]. cDNA was prepared from polyA+ mRNA using SuperScript Plasmid System Kit (Invitrogen). The double-strand cDNAs were fractionated in a sepharose CL-2B 40cm-columns and fragments sizing between 0.8 and 1.5 Kb were directionally cloned into the vector. Details of each source of RNA and library construction can be obtained at http://sucest.lad.ic.unicamp.br/public

text: Saccharum hybrid cultivar (mixed)

text: SalI

text: NotI


text: Universidade Estadual de Campinas

text: live

text: Generic

text: SP80-87432; PB5211 x P57150-4

text: An unidirectional cDNA library generated from [Developed inflorescence and rachis (20cm-long)]. cDNA was prepared from polyA+ mRNA using SuperScript Plasmid System Kit (Invitrogen). The double-strand cDNAs were fractionated in a sepharose CL-2B 40cm-columns and fragments sizing between 0.8 and 1.5 Kb were directionally cloned into the vector. Details of each source of RNA and library construction can be obtained at http://sucest.lad.ic.unicamp.br/public

text: Saccharum hybrid cultivar (mixed)

text: SalI

text: NotI


text: Universidade Estadual de Campinas

text: live

text: Generic

text: SP80-87432; PB5211 x P57150-4

text: An unidirectional cDNA library generated from [Developing inflorescence and rachis (10cm-long)]. cDNA was prepared from polyA+ mRNA using SuperScript Plasmid System Kit (Invitrogen). The double-strand cDNAs were fractionated in a sepharose CL-2B 40cm-columns and fragments sizing between 0.8 and 1.5 Kb were directionally cloned into the vector. Details of each source of RNA and library construction can be obtained at http://sucest.lad.ic.unicamp.br/public

text: Saccharum hybrid cultivar (mixed)

text: SalI

text: NotI


text: Universidade Estadual de Campinas

text: live

text: Generic

text: An unidirectional cDNA library generated from [seedlings inoculated with Herbaspirilum rubrisubalbicans]. cDNA was prepared from polyA+ mRNA using SuperScript Plasmid System Kit (Invitrogen). The double-strand cDNAs were fractionated in a sepharose CL-2B 40cm-columns and fragments sizing between 0.8 and 1.5 Kb were directionally cloned into the vector. Details of each source of RNA and library construction can be obtained at http://sucest.lad.ic.unicamp.br/public

text: Saccharum hybrid cultivar SP70-1143

text: SalI

text: NotI


text: Universidade Estadual de Campinas

text: live

text: Generic

text: An unidirectional cDNA library generated from [Lateral buds from field grown adult plants]. cDNA was prepared from polyA+ mRNA using SuperScript Plasmid System Kit (Invitrogen). The double-strand cDNAs were fractionated in a sepharose CL-2B 40cm-columns and fragments sizing between 0.8 and 1.5 Kb were directionally cloned into the vector. Details of each source of RNA and library construction can be obtained at http://sucest.lad.ic.unicamp.br/public

text: Saccharum hybrid cultivar SP80-3280

text: SalI

text: NotI


text: Universidade Estadual de Campinas

text: live

text: Generic

text: An unidirectional cDNA library generated from [Lateral buds from plants adult plants growing in greenhouse]. cDNA was prepared from polyA+ mRNA using SuperScript Plasmid System Kit (Invitrogen). The double-strand cDNAs were fractionated in a sepharose CL-2B 40cm-columns and fragments sizing between 0.8 and 1.5 Kb were directionally cloned into the vector. Details of each source of RNA and library construction can be obtained at http://sucest.lad.ic.unicamp.br/public

text: Saccharum hybrid cultivar SP80-3280

text: SalI

text: NotI


text: Universidade Estadual de Campinas

text: live

text: Generic

text: An unidirectional cDNA library generated from [Leaf roll from field grown adult plants (large insert library)]. cDNA was prepared from polyA+ mRNA using SuperScript Plasmid System Kit (Invitrogen). The double-strand cDNAs were fractionated in a sepharose CL-2B 40cm-columns and fragments sizing between 0.8 and 1.5 Kb were directionally cloned into the vector. Details of each source of RNA and library construction can be obtained at http://sucest.lad.ic.unicamp.br/public

text: Saccharum hybrid cultivar SP80-3280

text: SalI

text: NotI


text: Universidade Estadual de Campinas

text: live

text: Generic

text: An unidirectional cDNA library generated from [Leaf roll from field grown adult plants (small insert library)]. cDNA was prepared from polyA+ mRNA using SuperScript Plasmid System Kit (Invitrogen). The double-strand cDNAs were fractionated in a sepharose CL-2B 40cm-columns and fragments sizing between 0.8 and 1.5 Kb were directionally cloned into the vector. Details of each source of RNA and library construction can be obtained at http://sucest.lad.ic.unicamp.br/public

text: Saccharum hybrid cultivar SP80-3280

text: SalI

text: NotI


text: Universidade Estadual de Campinas

text: live

text: Generic

text: An unidirectional cDNA library generated from [Etiolated leaves from in vitro grown sedlings]. cDNA was prepared from polyA+ mRNA using SuperScript Plasmid System Kit (Invitrogen). The double-strand cDNAs were fractionated in a sepharose CL-2B 40cm-columns and fragments sizing between 0.8 and 1.5 Kb were directionally cloned into the vector. Details of each source of RNA and library construction can be obtained at http://sucest.lad.ic.unicamp.br/public

text: Saccharum hybrid cultivar (mixed)

text: SalI

text: NotI


text: Universidade Estadual de Campinas

text: live

text: Generic

text: An unidirectional cDNA library generated from [Pool of sugarcane tissues]. cDNA was prepared from polyA+ mRNA using SuperScript Plasmid System Kit (Invitrogen). The double-strand cDNAs were fractionated in a sepharose CL-2B 40cm-columns and fragments sizing between 0.8 and 1.5 Kb were directionally cloned into the vector. Details of each source of RNA and library construction can be obtained at http://sucest.lad.ic.unicamp.br/public

text: Pool of sugarcane tissues

text: Saccharum hybrid cultivar

text: SalI

text: NotI


text: Universidade Estadual de Campinas

text: live

text: Generic

text: An unidirectional cDNA library generated from [Root tips (0.3cm-long) from adult plants]. cDNA was prepared from polyA+ mRNA using SuperScript Plasmid System Kit (Invitrogen). The double-strand cDNAs were fractionated in a sepharose CL-2B 40cm-columns and fragments sizing between 0.8 and 1.5 Kb were directionally cloned into the vector. Details of each source of RNA and library construction can be obtained at http://sucest.lad.ic.unicamp.br/public

text: Saccharum hybrid cultivar SP80-3280

text: SalI

text: NotI


text: Universidade Estadual de Campinas

text: live

text: Generic

text: An unidirectional cDNA library generated from [Root tips(0.3cm-long) from adult plants]. cDNA was prepared from polyA+ mRNA using SuperScript Plasmid System Kit (Invitrogen). The double-strand cDNAs were fractionated in a sepharose CL-2B 40cm-columns and fragments sizing between 0.8 and 1.5 Kb were directionally cloned into the vector. Details of each source of RNA and library construction can be obtained at http://sucest.lad.ic.unicamp.br/public

text: Root tips(0.3cm-long) from adult plants

text: Saccharum hybrid cultivar SP80-3280

text: SalI

text: NotI


text: Universidade Estadual de Campinas

text: live

text: Generic

text: An unidirectional cDNA library generated from [Root apex from adult plants]. cDNA was prepared from polyA+ mRNA using SuperScript Plasmid System Kit (Invitrogen). The double-strand cDNAs were fractionated in a sepharose CL-2B 40cm-columns and fragments sizing between 0.8 and 1.5 Kb were directionally cloned into the vector. Details of each source of RNA and library construction can be obtained at http://sucest.lad.ic.unicamp.br/public

text: Root apex from adult plants

text: Saccharum hybrid cultivar SP80-3280

text: SalI

text: NotI


text: Universidade Estadual de Campinas

text: live

text: Generic

text: An unidirectional cDNA library generated from [Shoot-root transition zone from young plants (large insert library)]. cDNA was prepared from polyA+ mRNA using SuperScript Plasmid System Kit (Invitrogen). The double-strand cDNAs were fractionated in a sepharose CL-2B 40cm-columns and fragments sizing between 0.8 and 1.5 Kb were directionally cloned into the vector. Details of each source of RNA and library construction can be obtained at http://sucest.lad.ic.unicamp.br/public

text: Saccharum hybrid cultivar SP80-3280

text: SalI

text: NotI


text: Universidade Estadual de Campinas

text: live

text: Generic

text: An unidirectional cDNA library generated from [Shoot-root transition zone from young plants (small insert library)]. cDNA was prepared from polyA+ mRNA using SuperScript Plasmid System Kit (Invitrogen). The double-strand cDNAs were fractionated in a sepharose CL-2B 40cm-columns and fragments sizing between 0.8 and 1.5 Kb were directionally cloned into the vector. Details of each source of RNA and library construction can be obtained at http://sucest.lad.ic.unicamp.br/public

text: Saccharum hybrid cultivar SP80-3280

text: SalI

text: NotI


text: Universidade Estadual de Campinas

text: live

text: Generic

text: An unidirectional cDNA library generated from [Shoot-root transition zone from adult plants]. cDNA was prepared from polyA+ mRNA using SuperScript Plasmid System Kit (Invitrogen). The double-strand cDNAs were fractionated in a sepharose CL-2B 40cm-columns and fragments sizing between 0.8 and 1.5 Kb were directionally cloned into the vector. Details of each source of RNA and library construction can be obtained at http://sucest.lad.ic.unicamp.br/public

text: Saccharum hybrid cultivar SP80-3280

text: SalI

text: NotI


text: Universidade Estadual de Campinas

text: live

text: Generic

text: An unidirectional cDNA library generated from [Stalk Bark from adult plants]. cDNA was prepared from polyA+ mRNA using SuperScript Plasmid System Kit (Invitrogen). The double-strand cDNAs were fractionated in a sepharose CL-2B 40cm-columns and fragments sizing between 0.8 and 1.5 Kb were directionally cloned into the vector. Details of each source of RNA and library construction can be obtained at http://sucest.lad.ic.unicamp.br/public

text: Stalk Bark from adult plants

text: Saccharum hybrid cultivar SP80-3280

text: SalI

text: NotI


text: Universidade Estadual de Campinas

text: live

text: Generic

text: CB47-89; RB855205; RB845298; RB805028

text: An unidirectional cDNA library generated from [Developing seeds (large insert library)]. cDNA was prepared from polyA+ mRNA using SuperScript Plasmid System Kit (Invitrogen). The double-strand cDNAs were fractionated in a sepharose CL-2B 40cm-columns and fragments sizing between 0.8 and 1.5 Kb were directionally cloned into the vector. Details of each source of RNA and library construction can be obtained at http://sucest.lad.ic.unicamp.br/public

text: Developing seeds (large insert library)

text: Saccharum hybrid cultivar (mixed)

text: SalI

text: NotI


text: Universidade Estadual de Campinas

text: live

text: Generic

text: CB47-89; RB855205; RB845298; RB805028

text: An unidirectional cDNA library generated from [Developing seeds (small insert library)]. cDNA was prepared from polyA+ mRNA using SuperScript Plasmid System Kit (Invitrogen). The double-strand cDNAs were fractionated in a sepharose CL-2B 40cm-columns and fragments sizing between 0.8 and 1.5 Kb were directionally cloned into the vector. Details of each source of RNA and library construction can be obtained at http://sucest.lad.ic.unicamp.br/public

text: Developing seeds (small insert library)

text: Saccharum hybrid cultivar (mixed)

text: SalI

text: NotI


text: Universidade Estadual de Campinas

text: live

text: Generic

text: An unidirectional cDNA library generated from [First apical stalk internodes of adult plants]. cDNA was prepared from polyA+ mRNA using SuperScript Plasmid System Kit (Invitrogen). The double-strand cDNAs were fractionated in a sepharose CL-2B 40cm-columns and fragments sizing between 0.8 and 1.5 Kb were directionally cloned into the vector. Details of each source of RNA and library construction can be obtained at http://sucest.lad.ic.unicamp.br/public

text: Saccharum hybrid cultivar SP80-3280

text: SalI

text: NotI


text: Universidade Estadual de Campinas

text: live

text: Generic

text: An unidirectional cDNA library generated from [Fourth apical stalk internodes of adult plants]. cDNA was prepared from polyA+ mRNA using SuperScript Plasmid System Kit (Invitrogen). The double-strand cDNAs were fractionated in a sepharose CL-2B 40cm-columns and fragments sizing between 0.8 and 1.5 Kb were directionally cloned into the vector. Details of each source of RNA and library construction can be obtained at http://sucest.lad.ic.unicamp.br/public

text: Saccharum hybrid cultivar

text: SalI

text: NotI


text: Universidade Estadual de Campinas

text: live

text: Generic

text: Fourth apical stalk internodes of adult plants

text: Saccharum hybrid cultivar SP80-3280

text: SalI

text: NotI


text: Universidade Estadual de Campinas

text: live

text: Generic

text: An unidirectional cDNA library generated from [Developed inflorescence (20cm-long) without rachis]. cDNA was prepared from polyA+ mRNA using SuperScript Plasmid System Kit (Invitrogen). The double-strand cDNAs were fractionated in a sepharose CL-2B 40cm-columns and fragments sizing between 0.8 and 1.5 Kb were directionally cloned into the vector. Details of each source of RNA and library construction can be obtained at http://sucest.lad.ic.unicamp.br/public

text: Saccharum hybrid cultivar

text: SalI

text: NotI

text: Saccharum officinarum FL2


text: Universidade Estadual de Campinas

text: live

text: Generic

text: SP80-87432; PB5211 x P57150-4

text: An unidirectional cDNA library generated from [Base of developing inflorescence (5cm-long)]. cDNA was prepared from polyA+ mRNA using SuperScript Plasmid System Kit (Invitrogen). The double-strand cDNAs were fractionated in a sepharose CL-2B 40cm-columns and fragments sizing between 0.8 and 1.5 Kb were directionally cloned into the vector. Details of each source of RNA and library construction can be obtained at http://sucest.lad.ic.unicamp.br/public

text: Saccharum hybrid cultivar (mixed)

text: SalI

text: NotI

text: Saccharum officinarum FL3


text: Universidade Estadual de Campinas

text: live

text: Generic

text: SP80-87432; PB5211 x P57150-4

text: Developed inflorescence (20cm-long) without rachis

text: Saccharum hybrid cultivar (mixed)

text: SalI

text: NotI

text: Saccharum officinarum FL5


text: Universidade Estadual de Campinas

text: live

text: Generic

text: Saccharum hybrid cultivar

text: SalI

text: NotI

text: Saccharum officinarum FL6


text: Organization for Nucleotide Sequencing and Analysis

text: live

text: Generic

text: Saccharum hybrid cultivar

text: Saccharum officinarum library (de Mello MO)


text: Commonwealth Scientific and Industrial Research Organisation Division of Plant Industry

text: live

text: Generic

text: mRNA was prepared from roots of the sugarcane variety Q117 treated with 100ml of 200uM Methyl jasmonate. Treatments of 100ml of 200 uM methyl jasmonate in 1% ethanol solutions were applied to pots as a part of the normal watering regime for 4 consecutive days and roots sampled each day. cDNA was synthesised from a NotI-oligodT primer/adapter using the manufacturers protocols (Life Technologies) and then ligated to a SalI adapter to facilitate directional cloning. The cDNA was cloned into the SalI and NotI sites of the Lambda ZIPLOX phage vector (Life Technologies). Constructed by Neil I. Bower.

text: 3 months post-planting

text: roots

text: Saccharum hybrid cultivar Q117

text: SalI

text: NotI

text: Methyl jasmonate-treated root Lambda ZIPLOX Library (MJR)

text: Phage

text: Lambda ZIPLOX


text: University of Delhi South Campus

text: live

text: Generic

text: Co 1148

text: The cDNA library was constructed from polyA+ RNA by using the Clontech PCR-Select cDNA subtraction kit. The cDNAs were cloned randomly in pGEM-T Easy vector.

text: Mature

text: Saccharum hybrid cultivar Co 1148

text: Eco RI

text: Eco RI

text: hermaphrodite

characteristic: sex

text: Stem

text: Sugarcane red rot infected mature stem subtracted cDNA library

text: Plasmid

text: pGEM-T Easy


text: Vasantdada Sugar Institute

text: live

text: Generic

text: Co 740

text: messenger RNA was extracted from control and water stress induced sugarcane leaf tissue at 45 days after water stress induction and subtracted cDNA library was constructed following SSH method

text: Tillering stage

text: E.coli JM109

text: Saccharum hybrid cultivar Co 740

text: Leaf

text: Saccharum officinarum Expressed sequence tags from the Forward SSH library

characteristic: title

text: pGEM-T Easy vector


text: Vasantdada Sugar Institute

text: live

text: Generic

text: Co 740

text: messenger RNA was extracted from control and water deficit stress induced sugarcane leaf tissue at 30 days after water stress induction and subtracted cDNA library was constructed following SSH method

characteristic: description

text: Tillering stage

text: E.coli JM109

text: Saccharum hybrid cultivar Co 740

text: Leaf

text: Expressed sequence tags from the Forward SSH library 30 days after water stress induction

text: pGEM-T Easy vector


text: Bhabha Atomic Research Centre

text: live

text: Generic

text: Co 86032

text: Sugarcane plants at the stage of two months of planting were stressed with salt (200 mM NaCl) for 0.5 to 18 hrs. The root and shoot tissues of both the treated and control samples were used in PCR select cDNA SSH. The cDNA clones differentially expressed in salt stress were cloned and sequenced.

text: Two month post planting

text: E. coli

characteristic: lab-host

text: Root and Shoot

text: Saccharum hybrid cultivar Co 86032

text: Root and Shoot

text: Salt treated cDNA subtraction library PTZ57R/T

text: Plasmid


text: Bhabha Atomic Research Centre

text: live

text: Generic

text: Co 86032

text: Sugarcane plants at the stage of two months of planting were stressed with 200 mM NaCl for 0.5 to 18 hrs. The root and shoot tissues of both the treated and control samples were used in PCR select cDNA SSH. The cDNA clones differentially expressed in salt stress were cloned and sequenced.

text: Two month of post planting

text: E. coli

text: Root and Shoot

text: Saccharum hybrid cultivar Co 86032

text: Root and Shoot

characteristic: tissue-type

text: Salt treated cDNA subtraction library PCR Topo 4

text: Plasmid

text: PCR Topo 4


text: live

text: Generic

text: Genomic library without any microsatellite repeat-motifs prepared from young leaves of Saccharum hybrid cultivar cloned in pGEM-T Easy vector.

text: Seedling

text: XL-1 Blue

text: Saccharum hybrid cultivar Co 86011

text: Young leaves

text: Sugarcane cv. Co86011 size fractionated genomic library

text: Plasmid

text: pGEM-T Easy

characteristic: vector


characteristic: External Id

text: Ministry of Agriculture

text: live

text: Generic

text: In this study, two sugarcane varieties, NCo376 high resistant to smut and F134 susceptible to smut Race 2, were used to detect the differentially expressed genes between sugarcane material infected by U. scitaminea and control material.

text: Saccharum hybrid cultivar F134

text: leaf

text: Saccharum officinarum F134 ESTs from DDRT-PCR analysis


text: Ministry of Agriculture

characteristic: INSDC center name

text: live

text: Generic

text: Saccharum hybrid cultivar NCo 376

text: leaf

text: Saccharum officinarum NCo376 ESTs from DDRT-PCR analysis


text: Bhabha Atomic Research Centre

characteristic: INSDC first public

text: live

text: Generic

text: Co 86032

text: Sugarcane plants at the seedling stage were stressed with salt (200 mM NaCl) and PEG 8000 for 0.5 to 24 hrs. The total RNA isolated from leaf tissues of both the treated and control leaf samples were used in semi quantitative RT PCR. The PCR products were cloned and sequenced.

text: seedling

text: E. coli

text: Leaf

text: Saccharum hybrid cultivar Co 86032

text: Leaf

text: Sugarcane Salt and PEG stress treated cDNA

text: Plasmid


text: Vasantdada Sugar Institute

characteristic: INSDC last update

text: live

text: Generic

text: CoC 671

text: messenger RNA was extracted from healthy and SCGS infected sugarcane leaf tissue and subtracted cDNA library was constructed following SSH method

text: Tillering stage

text: E.coli DH5alpha

text: Saccharum hybrid cultivar CoC 671

text: Leaf

text: Saccharum officinarum CoC671 ESTs from the Forward SSH library

text: InsTA PCR cloning vector (Fermentas)


text: Bhabha Atomic Research Centre

text: live

text: Generic

text: Co 86032

characteristic: cultivar

text: Sugarcane plants at the seedling stage were stressed with salt (NaCl, 200mM) or PEG 8000 (20%) for 0.5 to 24 hrs. The total RNA isolated from leaf tissues and were used in RT PCR. The PCR products were cloned and sequenced.

text: seedling

text: E. coli

text: Leaf

text: Saccharum hybrid cultivar Co 86032

text: Leaf

text: Sugarcane Salt or PEG stress treated cDNA

text: Plasmid


text: Bhabha Atomic Research Centre

text: live

characteristic: INSDC status

text: Generic

text: Co 62175

text: Sugarcane plants at 60 days after sowing stage were stressed with salt (150mM NaCl) or PEG 8000 (20%) for 15 Days. The total RNA isolated from leaf tissues were used in RT PCR. The PCR products were cloned and sequenced.

text: seedling

text: E. coli

text: Leaf

text: Saccharum hybrid cultivar Co 62175

text: Leaf

text: Sugarcane Co-62175 Salt or PEG stress treated cDNA

text: Plasmid


text: Bhabha Atomic Research Centre

text: live

text: Generic

characteristic: NCBI submission model

text: Co 86032

text: Sugarcane plants at four months post planting stage were stressed with salt (150mM NaCl) for 4 hrs and 8 hrs. The total RNA isolated from leaf tissues were used in RT PCR. The PCR products were cloned and sequenced.

text: Four months post planting

text: E. coli

text: Leaf

text: Saccharum hybrid cultivar Co 86032

text: Leaf

text: Sugarcane plants salt stressed at four months

text: Plasmid


text: Vasantdada Sugar Institute

text: live

text: Generic

characteristic: NCBI submission package

text: Co 62175

text: Messenger RNA was extracted from healthy and SCGS infected sugarcane leaf tissue and subtracted library was constructed following SSH method

text: Tillering stage

text: E.coli DH5alpha

text: Saccharum hybrid cultivar Co 62175

text: Leaf

text: Sugarcane cv. Co62175 Forward SSH library

text: InsTA PCR cloning vector (Fermentas)


text: University of Northern Colorado

text: live

text: Generic

text: young Salsola iberica growing in parking lot crack, full sun

text: Salsola tragus

text: leaves and stem

text: immature tumbleweed


text: Vasantdada Sugar Institute

text: live

text: Generic

text: CoC 671

text: Tillering stage

characteristic: dev-stage

text: E.coli DH5alpha

text: Saccharum hybrid cultivar CoC 671

text: Leaf

text: Sugarcane cv. CoC 671 Forward SSH library

text: InsTA PCR cloning/pGEMT Easy vector (Fermentas/Promega)


text: National Research Council Canada Plant Biotechnology Institute

text: live

text: Generic

text: Sulfuric acid-treated B. orellana L. seeds Chiltern Seed Co., Ulverston, England) were geminated and grown in soil under greenhouse conditions with 18 h of light at 22-24 C. Seed coats from immature B. orellana seed pods were separated from their seeds by rapidly stirring 5 g of seeds for 45 min at 4 C in 8.5 ml of extraction buffer. Seeds and debris were pelleted by centrifugation at 2000 x/g for 5 min. RNA was then isolated from the supernatant and from 100 mg of young leaves. Copy DNA synthesis was performed using the SMART PCR cDNA Synthesis Kit (Clontech). The cDNA population was then enriched in seed coat specific sequences by subtraction with leaf cDNA using the PCR-Select cDNA Subtraction Kit (Clontech). The subtracted seed coat cDNA population was ligated into the pCRII-TOPO cloning vector (Invitrogen). The plasmid population was transformed into ElectroMax DH10B cells (Gibco BRL). The resulting 2.0 x 10^4 colonies were pooled and stored as a glycerol stock.

text: E. coli

text: developing seed

characteristic: organ

text: Bixa orellana

text: seed coat

text: BIXA

text: Plasmid

text: pPCRII-TOPO


text: live

text: Generic

text: H. niger (seeds from Plant Gene Resource of Canada, Saskatoon)was grown in soil with 16 hr/23C-24C days and 8 hr/20C nights under 100 umol/m2/s light intensity. Cultured roots were grown at 25C C in the dark on a rotary shaker (100 rpm) in Gamborg B5 medium containing 3% (w/v) sucrose and 1 mM indole-3-butyric acid. The polyA+ RNA fraction was isolated with the PolyATract mRNA Isolation System (Promega,Madison, WI). Double-stranded cDNAs were synthesized, and suppression PCR was conducted by using the PCR-Select cDNA Subtraction Kit (Clontech, Mountain View, CA) with root cDNA as tester and leaf cDNA as driver.

text: E. coli

text: cultured root minus leaf

text: Hyoscyamus niger

characteristic: organism

text: HNSUB

text: Plasmid

text: pPCRII-TOPO


text: live

text: Generic

text: mRNA was converted in to cDNAs and fingerprinting was done using RAPD primers. The fragments which have shown consitant amplification only in infected cDNAs were cut, eluted, cloned and sequenced.

text: Candidatus Phytoplasma sacchari

text: cDNA fragment of SCGS from the Forward SSH library


text: Institute of Himalayan Bioresource Technology

text: live

text: Generic

text: Leaf

text: Caragana jubata

text: Leaf

text: Caragana jubata reverse (Control; 25C) suppression subtractive hybridization (SSH) library

text: plasmid

text: pGEM-T Easy (Promega)


text: University of Heilongjiang

text: live

text: Generic

text: mycorrhizal root cell

characteristic: cell type

text: EST obtained through DDRT-PCR. Arbuscular mycorrhizal fungi (Glomus mosseae) and plant Amorpha fruticosa.

text: 28 day after inoculation

text: Amorpha fruticosa/Glomus mosseae mixed EST library

text: Amorpha fruticosa/Glomus mosseae arbuscular mycorrhiza 28 days


text: University of Basilicata

text: live

text: Generic

text: Fragaria vesca

text: roots

text: cDNA from Fragaria vesca roots treated with organic fertilizer

characteristic: title


text: University of Basilicata

text: live

text: Generic

text: Fragaria vesca

text: leaves

text: cDNA from Fragaria vesca leaves treated with organic fertilizer


text: University of Basilicata

text: live

text: Generic

text: Fragaria vesca

text: leaves

text: cDNA from Fragaria vesca leaves treated with inorganic fertilizer


text: University of Basilicata

text: live

text: Generic

text: Fragaria vesca

text: roots

text: cDNA from Fragaria vesca roots treated with inorganic fertilizer


text: Universidad de Valencia

text: live

text: Generic

text: mRNA was extracted from two strains of Aspergillus carbonarius antagonists for OTA production. The PCR-Select cDNA Subtraction Kit (Clontech) has been used to generate this specific cDNA library. The cDNA from the OTA producer strain W04-40 was used as a tester and the cDNA from de OTA non producer W04-46 was used as driver.

characteristic: description

text: 2 day old solid media

text: E.coli DH5alpha

text: Aspergillus carbonarius

characteristic: strain

text: mycelia

text: Aspergillus carbonarius SSH Library

text: plasmid

text: pCR 2.1


text: live

text: Generic

text: Plants (seed source: Elixir Farm Botanicals, Brixey,MO, USA) were grown with 16 h/25 C days and 8 h/20 C nights to a height of approximately 1.2 m (about 3 months) then transferred to 12 h/25 C days and 12 h/20 C nights. cDNA synthesis and library construction were carried out with a Creator SMART cDNA Library Construction Kit (Clontech) using Long-Distance PCR.

text: 19-21 days after induction of flowering

text: E. coli

characteristic: lab-host

text: flower bud

text: Artemisia annua

text: AAFB

text: Plasmid

text: pDNR-LIB


text: live

text: Generic

text: Plants (seed source: Elixir Farm Botanicals, Brixey,MO, USA) were grown with 16 h/25 C days and 8 h/20 C nights to a height of approximately 1.2 m (about 3 months) then transferred to 12 h/25 C days and 12 h/20 C nights. Glandular trichomes were isolated by abrasion using a Bead Beater (Biospec) and filtration through nylon mesh. cDNA synthesis and library construction were carried out with a Creator SMART cDNA Library Construction Kit (Clontech) using Long-Distance PCR.

text: 19-21 days after induction of flowering

text: E. coli

text: flower bud

text: Artemisia annua

text: glandular trichome

characteristic: tissue-type

text: AAGST

text: Plasmid

text: pDNR-LIB

characteristic: vector


characteristic: External Id

text: National Research Council Canada Plant Biotechnology Institute

characteristic: INSDC center name

text: live

text: Generic

text: Plants (seed source: Elixir Farm Botanicals, Brixey,MO, USA) were grown with 16 h/25 C days and 8 h/20 C nights to a height of approximately 1.2 m (about 3 months) then transferred to 12 h/25 C days and 12 h/20 C nights. Glandular trichomes were isolated by abrasion using a Bead Beater (Biospec) and filtration through nylon mesh. A subtracted cDNA library was constructed with a PCR-Select cDNA Subtraction Kit (Clontech) using poly A+ RNA isolated from glandular trichomes (tester) and flower buds (driver).

text: 19-21 days after induction of flowering

text: E. coli

text: flower bud

text: Artemisia annua

text: glandular trichome minus flower bud

text: GSTSUB

text: Plasmid

text: pDNR-LIB


text: University of Basilicata

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Fragaria x ananassa

text: roots

text: cDNA from Fragaria x ananassa roots treated with inorganic fertilizer


text: University of Basilicata

text: live

characteristic: INSDC status

text: Generic

text: Fragaria x ananassa

text: roots

text: cDNA from Fragaria x ananassa roots treated with organic fertilizer


text: University of Basilicata

text: live

text: Generic

characteristic: NCBI submission model

text: Fragaria x ananassa

text: leaves

text: cDNA from Fragaria x ananassa leaves treated with organic fertilizer


text: University of Basilicata

text: live

text: Generic

characteristic: NCBI submission package

text: Fragaria x ananassa

text: leaves

text: cDNA from Fragaria x ananassa leaves treated with inorganic fertilizer


text: Universidad de Oviedo

text: live

text: Generic

text: Root apex subtractive library of PEG-stressed E. globulus versus non PEG-stressed

text: Root

text: Eucalyptus globulus

text: C14 clone (ENCE)

text: Apex

text: Eucalyptus globulus C14 clone root apex under water stress

text: pGEMTeasy


text: Genoscope - Centre National de Sequencage

text: live

text: Generic

text: EST library from conjugating spores of the Microbotryum species parasitizing the plant Dianthus cartusianorum (Microbotryum carthusianorum)

text: Microbotryum violaceum

text: Microbotryum violaceum 100.02 (Dianthus cartusianorum)

text: pGEM(R)-T


text: live

text: Generic

text: EST library from conjugating spores of the Microbotryum species parasitizing the plant Silene vulgaris (Microbotryum /lagerheimi/)

text: Microbotryum violaceum

text: Microbotryum violaceum 100.02 (Silene vulgaris)

text: pGEM(R)-T


text: live

text: Generic

text: EST library from conjugating spores of the Microbotryum species parasitizing the plant Silene nutans (Microbotryum violaceum s.str.)

text: Microbotryum violaceum

text: Microbotryum violaceum 100.02 (Silene nutans)

text: pGEM(R)-T


text: Zoological Museum of the University of Hamburg

text: live

text: Generic

text: adult colony

characteristic: dev-stage

text: Alcyonidium diaphanum

text: colony lobe

text: Alcyonidium diaphanum EST library


text: live

text: Generic

text: adult colony

text: Tubulipora sp. BH-2010a

text: whole colony

text: Tubulipora sp. EST library

text: pDNR-LIB


text: Bioengineering College of Chongqing University

text: live

text: Generic

text: We selected Mylabris Cichorii Linnaeus adults as materials to construct the cDNA library by SMART technology,using PDNR-Lib as vector.

text: separate reared beetles after emergence for 20-30 days

text: Mylabris cichorii

text: SfiI

text: SfiI

text: male

characteristic: sex

text: cDNA library of Mylabris Cichorii Linnaeus

text: Plasmid

text: PDNR-Lib


text: Universidade Federal de Vicosa

text: live

text: Generic

text: This is a suppression subtractive hybridization library in which C. lindemuthianum in appressorium formation is the tester and C. lindemuthianum mycelium is the driver. The subtracted sequences were cloned in pGEM vector.

text: initial phase of infection in Phaseolus vulgaris

text: Colletotrichum lindemuthianum

characteristic: organism

text: 89 race, A2 2-3

text: Appressorium formation in spores

text: Colletotrichum lindemuthianum appressorium formation Library

text: plasmid

text: pGEM


text: Universidade Federal de Vicosa

text: live

text: Generic

text: 89 race of Colletotrichum lindemuthianum; Rosinha cultivar of Phaseolus vulgaris. This is a suppression subtractive hybridization library in which Phaseolus vulgaris leaves infected with C. lindemuthianum (3 days after infection) are the tester and C. lindemuthianum mycelium is the driver. The subtracted sequences were cloned in pGEM vector.

text: biotrophic phase - 3 days post infection

text: Phaseolus vulgaris/Colletotrichum lindemuthianum mixed EST library

text: Colletotrichum lindemuthianum-Phaseolus vulgaris (3 days post infection) Library

text: plasmid

text: pGEM


text: Universidade Federal de Vicosa

text: live

text: Generic

text: 89 race of Colletotrichum lindemuthianum; Rosinha cultivar of Phaseolus vulgaris. This is a suppression subtractive hybridization library in which Phaseolus vulgaris leaves infected with C. lindemuthianum (5 days after infection) are the tester and C. lindemuthianum mycelium is the driver. The subtracted sequences were cloned in pGEM vector.

text: necrotrophic phase - 5 days post infection

text: Colletotrichum lindemuthianum-Phaseolus vulgaris (5 days post infection) Library

text: plasmid

text: pGEM


text: Universidade Federal de Vicosa

text: live

text: Generic

text: 89 race of Colletotrichum lindemuthianum; Rosinha cultivar of Phaseolus vulgaris. This is a suppression subtractive hybridization library in which Phaseolus vulgaris leaves infected with C. lindemuthianum (7 days after infection) are the tester and C. lindemuthianum mycelium is the driver. The subtracted sequences were cloned in pGEM vector.

text: necrotrophic phase - 7 days post infection

text: Colletotrichum lindemuthianum-Phaseolus vulgaris (7 days post infection) Library

text: plasmid

text: pGEM


text: Southwest Hospital

text: live

text: Generic

text: newborn

text: dermis

characteristic: organ

text: Rattus norvegicus

text: Wistar

text: dermis

text: wound healing related library


text: Shiraz University

text: live

text: Generic

text: Karoon

characteristic: cultivar

text: Seedlings of barley were grown for 10 days. Isolation was carried out from leaves and by specific primers via PCR.

text: 10 days seedlings under normal condition

text: Leaf

text: Hordeum vulgare

text: Hordeum vulgare cv. Karoon 10 days seedlings

characteristic: title


text: Anhui Agricultural Univerisity

text: live

text: Generic

text: Longjin 43

text: The leaves of cutting seedlings (2 year) were fed by Tea Looper (Ectropic oblique), the unattacked leaves were control. Subtractive library: Driver = RNA from control leaves; Tester = RNA from the attacded leaves.

characteristic: description

text: E. coli DH5a

text: Camellia sinensis

text: Leaf

text: SSH library of leaves induced by Ectropic oblique feeding


text: National Chemical Laboratory

text: live

text: Generic

text: Foc race 1. Foc inoculated root tissue harvested at different time points (2- 16 days post inoculation). RNA extraction using Trizol method followed by cDNA conversion. Amplification of cDNA using gene specific primers and sequencing.

text: Fusarium oxysporum f. sp. ciceris

text: race 1

characteristic: strain

text: Foc inoculated chickpea root tissue

text: Fusarium oxysporum f. sp ciceris infected chickpea roots (Foc)


text: National Chemical Laboratory

text: live

text: Generic

text: Digvijay

text: Cicer arietinum

text: Foc inoculated chickpea root tissue

characteristic: tissue-type

text: Fusarium oxysporum f. sp ciceris infected chickpea roots (C. arietinum)


text: College of Animal Science and Technology, SiChuan Agricultrual University

text: live

text: Generic

text: hepatocyte

characteristic: cell type

text: Up- and down-regulation Genes in Goose Hepatocyte exposed to Free Fatty Acid

characteristic: lab-host

text: liver

text: Anser cygnoides

text: male

text: Sichuan White goose

text: Differentialy Expressed Genes in Goose Hepatocyte exposed to Free Fatty Acid

text: pBluescript-T

characteristic: vector


characteristic: External Id

text: National Taiwan University

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: Pacifica

text: Differential display was applied to isolate Catharanthus roseus cDNAs differentially expressed following infection with peanut witches' broom (PnWB) phytoplasma. Differentially expressed cDNA fragments were reamplified, cloned, and sequenced.

text: TOP10F' (Invitrogen)

text: Catharanthus roseus

text: Hermaphrodite

text: Leaf

text: Catharanthus roseus differential display cDNA clone

text: Plasmid

text: pCRII-TOPO (Invitrogen)


text: Norwegian School of Veterinary Science

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: cell-line

text: Subtractive library from CHSE-214 cells persistently infected with IPNV. Tester: Persistently infected CHSE cells, driver: non-infected CHSE cells. CLONTECH PCR-Select cDNA Subtraction Kit

text: Oncorhynchus tshawytscha

text: CHSE-214 cells persistently infected with IPNV library

text: Plasmid

text: pDrive (Qiagen)


text: Wellcome Trust Sanger Institute

text: live

text: Generic

characteristic: NCBI submission package

characteristic: isolate

text: Plasmodium falciparum 3D7

characteristic: organism


text: Agricultural Genetic Engineering Research Institute (AGERI)

text: live

text: Generic

text: country: Egypt: Giza ; collected_by: Rania Abou Ali ; identified_by: Rania Abou Ali

text: Vicia villosa

text: Vicia villosa Giza library

characteristic: title


text: British Antarctic Survey

text: live

text: Generic

text: Subtracted liver library made from specimens held at 4 degree celsius

characteristic: description

text: Liver

characteristic: organ

text: Harpagifer antarcticus


text: British Antarctic Survey

text: live

text: Generic

text: Subtracted liver library made from specimens held at 6 degree celsius

text: Liver

text: Harpagifer antarcticus


text: British Antarctic Survey

text: live

text: Generic

text: Subtracted liver library made from specimens held at 8 degree celsius

text: Liver

text: Harpagifer antarcticus


text: British Antarctic Survey

text: live

text: Generic

text: Subtracted liver library made from specimens held at 15 degree celsius

text: Liver

text: Harpagifer antarcticus


text: Vasantdada Sugar Institute

text: live

text: Generic

text: Co 740

characteristic: cultivar

text: messenger RNA was extracted from control and salt stress induced sugarcane leaf tissue at 15 days after planting and transcript-derived fragments were obtained by AP-PCR method

text: Germination stage

characteristic: dev-stage

text: E.coli JM109

text: Saccharum hybrid cultivar Co 740

text: Leaf

characteristic: tissue-type

text: sugarcane leaf tissue from germinating seedlings at 15 days after planting

text: pTZ T/A cloning vector


text: University of Agricultural Sciences

text: live

text: Generic

text: Two months old Coffee seedlings were subjected to different water status (30% FC and 60%FC) by withholding irrigation till the respective stress level is reached. Total RNA was isolated from young stressed leaf tissues and total stress cDNA library was constructed using modified TA cloning strategy.

text: E.coli

characteristic: lab-host

text: Coffea canephora

text: Eco RI

text: Young Leaf

text: Coffee drought stressed leaf cDNA library

text: pGEM-T Easy vector

characteristic: vector


characteristic: External Id

text: University of Minnesota

characteristic: INSDC center name

text: live

text: Generic

text: spleen

text: Haemorhous mexicanus

text: male

characteristic: sex

text: House Finch spleen SSH cDNA library


text: National Research Centre on Plant Biotechnology

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Asha

text: cDNA Library prepared from Bud tissue in pGEMT vector using clonetech c-DNA synthesis kit

text: Immature

text: XL-1 Blue

text: Cajanus cajan

text: Bud

text: Pigeonpea Asha BUD cDNA Library

text: Plasmid

text: pGEMT


text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Scarabaeoides

text: cDNA Library prepared from heat stressed plant tissue in pGEMT vector using clonetech c-DNA synthesis kit.

text: 6 weeks

text: XL-1 Blue

text: Cajanus cajan

text: plant

text: Pigeonpea Scarabaeoides heat stressed cDNA Library

text: Plasmid

text: pGEMT


text: live

text: Generic

text: Immature

text: XL-1 Blue

text: Cajanus cajan

characteristic: organism

text: Bud

text: Pigeonpea UPAS120 BUD cDNA Library

text: Plasmid

text: pGEMT


text: live

text: Generic

text: The tissue samples were harvested under drought stress conditions and cDNA library was constructed in pGEMT vector using clonetech c-DNA synthesis kit.

text: 4 weeks

text: XL-1 Blue

text: Cajanus cajan

text: Plant

text: Pigeonpea UPAS120 drought stressed cDNA Library

characteristic: title

text: Plasmid

text: pGEMT


text: live

text: Generic

text: The tissue samples were harvested under flooding stress conditions and cDNA library was constructed in pGEMT vector using clonetech c-DNA synthesis kit.

characteristic: description

text: 4 weeks

text: XL-1 Blue

text: Cajanus cajan

text: Plant

text: Pigeonpea UPAS120 flood stressed cDNA Library

text: Plasmid

text: pGEMT


text: live

text: Generic

text: 6 weeks

text: XL-1 Blue

text: Cajanus cajan

text: plant

text: Pigeonpea UPAS120 heat stressed cDNA Library

text: Plasmid

text: pGEMT


text: live

text: Generic

text: cDNA Library prepared from Methyl jasmonate treated leaf tissue in pGEMT vector using clonetech c-DNA synthesis kit

text: 4 weeks

text: XL-1 Blue

text: Cajanus cajan

text: Leaf

text: Pigeonpea UPAS120 Methyl jasmonate treated cDNA Library

text: Plasmid

text: pGEMT


text: live

text: Generic

text: cDNA Library prepared from Salicylic acid treated leaf tissue in pGEMT vector using clonetech c-DNA synthesis kit

text: 4 weeks

text: XL-1 Blue

text: Cajanus cajan

text: Leaf

text: Pigeonpea UPAS120 salicylic acid treated cDNA Library

text: Plasmid

text: pGEMT


text: live

text: Generic

text: Asha

text: 4 weeks

text: XL-1 Blue

text: Cajanus cajan

text: Plant

text: Pigeonpea Asha drought stressed cDNA Library

text: Plasmid

text: pGEMT


text: live

text: Generic

text: Asha

text: 4 weeks

text: XL-1 Blue

text: Cajanus cajan

text: Plant

text: Pigeonpea Asha flood stressed cDNA Library

text: Plasmid

text: pGEMT


text: live

text: Generic

text: Asha

text: cDNA Library prepared from leaf tissue in pGEMT vector using clonetech c-DNA synthesis kit

text: 4 weeks

text: XL-1 Blue

text: Cajanus cajan

text: leaf

text: Pigeonpea Asha leaf cDNA Library

text: Plasmid

text: pGEMT


text: live

text: Generic

text: Asha

text: 6 weeks

text: XL-1 Blue

text: Cajanus cajan

text: plant

text: Pigeonpea Asha heat stressed cDNA Library

text: Plasmid

text: pGEMT


text: National Research Centre on Plant Biotechnology

text: live

text: Generic

text: Asha

text: cDNA Library prepared from root tissue in pGEMT vector using clonetech c-DNA synthesis kit

text: Immature

text: XL-1 Blue

text: Cajanus cajan

text: root

text: Pigeonpea Asha root cDNA Library

text: Plasmid

text: pGEMT


text: live

text: Generic

text: Asha

text: 4 weeks

text: XL-1 Blue

text: Cajanus cajan

text: Leaf

text: Pigeonpea Asha salicylic acid treated cDNA Library

text: Plasmid

text: pGEMT


text: live

text: Generic

text: Scarabaeoides

text: 4 weeks

text: XL-1 Blue

text: Cajanus cajan

text: Plant

text: Pigeonpea Scarabaeoides drought stressed cDNA Library

text: Plasmid

text: pGEMT


text: live

text: Generic

text: Scarabaeoides

text: 4 weeks

text: XL-1 Blue

text: Cajanus cajan

text: Plant

text: Pigeonpea Scarabaeoides flood stressed cDNA Library

text: Plasmid

text: pGEMT


text: Agricultural University of Hebei

text: live

text: Generic

text: estrus period

text: Capra hircus

text: female

text: ovary

text: goat estrus period ovary tissue Library


text: Chongqing University

text: live

text: Generic

text: adult 12-48 hours post-infection by Metarhizium anisopliae

text: Locusta migratoria manilensis

text: male

text: fat body and hemocyte

text: Fat body and hemocyte of Locusta migratoria naturally infected by Metarhizium anisopliae


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 0.6-1.2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed at DOE Joint Genome Institute (Walnut Creek, CA).

text: Thermobifida fusca YX

text: SalI

text: NotI

text: YX

characteristic: strain

text: CACP Thermobifida fusca YX thefu_mRNA L


text: DOE Joint Genome Institute

text: live

text: Generic

text: Thermobifida fusca YX

text: SalI

text: NotI

text: YX

text: CACU Thermobifida fusca YX thefu_R1 L


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: grown at 25C in the light. The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected >2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed at DOE Joint Genome Institute (Walnut Creek, CA).

text: 4-day-old colony

text: Schizophyllum commune

text: SalI

text: NotI

text: monokaryotic

text: H 4-8

text: whole mycelium

text: CBXX Schizophyllum commune 4-day-old colony, grown at 25 C in the light (H)


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: grown at 25C in the light. The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected 0.5-2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed at DOE Joint Genome Institute (Walnut Creek, CA).

text: 4-day-old colony

text: Schizophyllum commune

text: SalI

text: NotI

text: monokaryotic

text: H 4-8

text: whole mycelium

text: CBXY Schizophyllum commune 4-day-old colony, grown at 25 C in the light (L)


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: grown at 25C in the dark (4-day-old colony) and in the light (8-day-old colony). The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected >2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed at DOE Joint Genome Institute (Walnut Creek, CA).

text: 4-day-old colony (dark) and 8-day-old colony (light)

text: Schizophyllum commune

text: SalI

text: NotI

text: dikaryotic

text: H 4-8 x 4-8.3

text: whole mycelium

text: CBXZ Schizophyllum commune pool of 4- and 8-day-old colony, grown at 25 C in resp. the dark and in light (H)


text: Wageningen University

text: live

text: Generic

text: Total RNA was obtained from fecal samples of 2 indivuduals as published by Zoetendal et al., 2007 (Nat. Protocols 1: 954-959). Subsequent mRNA enrichment was performed by MicrobExpress (Ambion). Two time samples (1 day apart) were profiled by cDNA-AFLP. Subsequent sequence analysis of free-laying bands was performed after reamplificaiton with the same selective primers used for cDNA-AFLP profiling.

text: human gut metagenome

text: Fecal samples of 2 healthy individuals


text: Korea Forest Research Institute

text: live

text: Generic

text: Subtraction Suppression Hybridization library (Clontech PCR Select) generated by forward subtracting transcripts of suspension cell of 2 hr or 10 hr salinity (150 mM NaCl) treated poplar.

text: JM109 strain

text: Populus alba x Populus glandulosa

text: EcoRI

text: Suspension cell

text: cDNA library from NaCl treated poplar cells

text: plasmid

text: pGEM-T easy


text: USDA-ARS

text: live

text: Generic

text: Subtractive hybridization was performed with the PCR-Select cDNA Subtraction Kit (Clontech), according to the manufacture's protocol. Tester tissue consisted of the whole fry fish 10 minutes post vaccination with Flavobacterium columnare vaccine (Aquavac-COL). Driver tissue was the non-vaccinated whole fry fish.

text: 7 to 10 days post hatch

text: whole fish

characteristic: organ

text: Ictalurus punctatus

text: mixed

text: Channel catfish fry subtractive library 10 min post vaccination with Flavobacterium columnare vaccine (Aquavac-COL)

text: pGEM T easy (promega)


text: Florida A & M University

text: live

text: Generic

text: Zinfandel

characteristic: cultivar

text: Used Subtractive Hybridization to isolated transcripts induced specifically upon Xylella fastidiosa infestation.

text: Vitis vinifera

text: Leaf

text: Pierce's Disease induced genes (Zinfandel) leaf


text: Florida A & M University

text: live

text: Generic

text: Noble

text: Reverse transcriptase PCR to amplify genes from RNA isolated from ripened muscadine berry

text: Vitis rotundifolia

text: Berry

characteristic: tissue-type

text: Genes involved in sugar breakdown in muscadine grape


text: University Putra Malaysia

text: live

text: Generic

text: Mimi Palmer

text: Vanda hybrid cultivar

text: floral

text: Vanda Mimi Palmer floral cDNA library


text: University of Washington

text: live

text: Generic

text: all

characteristic: dev-stage

text: Caenorhabditis elegans

text: whole worm

text: Caenorhabditis elegans whole worm N2 all stages


text: University of Delhi South Campus

text: live

text: Generic

text: CoS 767

text: The cDNA library was constructed from polyA+ RNA by using the Clontech SMART cDNA synthesis kit. The cDNAs were cloned directionally in pDNR-LIB vector.

text: Immature

text: Saccharum hybrid cultivar CoS 767

text: Sfi I

text: Sfi I

text: hermaphrodite

text: Leaf whorl

text: Sugarcane leaf whorl library

text: Plasmid

text: pDNR-LIB

characteristic: vector


characteristic: External Id

text: University of Delhi South Campus

text: live

text: Generic

text: CoS 767

text: mature

text: Saccharum hybrid cultivar CoS 767

text: Sfi I

text: Sfi I

text: hermaphrodite

text: stem

text: Sugarcane mature stem library

text: Plasmid

text: pDNR-LIB


text: University of Delhi South Campus

characteristic: INSDC center name

text: live

text: Generic

text: CoS 767

text: The cDNA library was constructed from polyA+ RNA by using the ZAP Express cDNA synthesis kit. The cDNAs were cloned directionally in ZAP Express vector. The cDNAs were excised from ZAP Express vector in the form of pBK-CMV phagemids.

text: Mature

text: Saccharum hybrid cultivar CoS 767

text: Eco RI

text: Xho I

text: hermaphrodite

text: Mature leaf tissue

text: Sugarcane mature leaf tissue library

text: Phagemid

text: pBK-CMV


text: University of Delhi South Campus

text: live

text: Generic

text: CoS 767

text: Mature

text: Saccharum hybrid cultivar CoS 767

text: Sfi I

text: Sfi I

text: hermaphrodite

characteristic: sex

text: Root

text: Sugarcane root library

text: Plasmid

text: pDNR-LIB


text: Hainan Medical College

characteristic: INSDC first public

text: live

text: Generic

text: mRNA was extracted from the adult Spirometra erinaceieuropaei and the full-length cDNA library was constructed by SMART method using pBluescript SK vector

text: adult

text: dog

characteristic: lab-host

text: Spirometra erinaceieuropaei

text: Sfi I A: 5'-GGCCATTACGGCC-3'

text: Sfi I B: 5'-GGCCGCCTCGGCC-3'

text: hermaphrodite

text: Hainan

text: whole tissue

text: full-length cDNA library of adult Spirometra erinaceieuropaei

text: Phagemid

text: pBluescript II SK


text: College of Natural Resources and Environment

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Total RNA was isolated from different developmental stages. The mRNA was subsequently converted to double-stranded cDNA. After SSH the PCR amplification products were cloned into the pGEM-T vector. The ligation mixture was used to transform Escherichia coli JM109 competent cells.

text: Mixed

text: Radopholus similis

text: female

text: Nematode

text: Different pathogenic populations of Radopholus similis SSH Library

text: Cosmid

text: pGEM-T easy vector


text: Embrapa Recursos Geneticos e Biotecnologia

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: A mini-library was made by cloning products derived from ORESTES PCR profiles into the pGEM-Teasy vector. Reverse transcription of parasite mRNA and cDNA amplification were performed under low stringency conditions

text: Amastigote and trypomastigote

text: Trypanosoma cruzi

text: VL 10

text: Amastigote and trypomastigote cDNA - ORESTES

text: plasmid

text: pGEM T easy


text: Pukyong National University

text: live

text: Generic

text: Miamiensis avidus

characteristic: organism

text: subtracted cDNA library of parasitic phase Miamiensis avidus

characteristic: title

text: pGEM T-easy vector


text: Forestry and Forest Products Research Institute

text: live

text: Generic

text: adult

text: Castanopsis sieboldii

text: inner bark

text: Castanopsis sieboldii inner bark cDNA library


text: INRA

text: live

text: Generic

text: one year old cuttings

text: Quercus robur

text: green leaves

text: SSH Quercus robur one year old cuttings


text: College of Agronomy, Shandong Agricultural University

text: live

text: Generic

text: Distal spikelet sterility mutant was obtained from the EMS (ethyl methane sulfonate) induced Shannong1186. The investigation on agronomic traits showed that 2~4 distal spikelets of the mutant was sterile. However, the distal spikelets of Shannong1186 were fertile. Observation on spikelet morphological development showed that distal spikelets of the mutant began to degrade in tetrad stage. Both mRNA differential display and silver staining techniques were used to isolate the distal spikelet sterility mutant cDNAs.

characteristic: description

text: Wheat panicles in tetrad stage

text: Triticum aestivum

characteristic: strain

text: Wheat panicles in tetrad stage Library

text: Plasmid

text: pGEM-T


text: Michigan State University

text: live

text: Generic

text: Elliott

characteristic: cultivar

text: mature

text: Vaccinium corymbosum

text: fruit

text: Vaccinium corymbosum SSH-C.acutatum-resistance