characteristic: External Id

text: Bioengineering College of Chongqing University

text: live

text: Generic

text: We selected Plutella xylostella adults as materials to construct the cDNA library by SMART technology, using PDNR-Lib as vector.

text: the second instar larvae of Plutella xylostella which were caught in the field

text: Plutella xylostella

text: SfiI

text: SfiI

text: male

text: cDNA library of Plutella xylostella

text: Plasmid

text: PDNR-Lib

characteristic: vector


status: PUBLIC

text: Bioengineering College, Chongqing University

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: The subtractive hybridization protocol from BD Bioscience Clontech (Palo Alto, CA) has been used to generate this specific cDNA library. Tester cDNA was prepared from fungus that was germinating and differentiating on locust wings and driver cDNA derived from the purified hyphal bodies from infected hemolymph in vivo. The resulting cDNAs were cloned into PMD-18T vector.

characteristic: description

text: Metarhizium acridum

characteristic: organism

tag: attribute

characteristic: strain

text: fungal bodie SSH Library during germination and differentiation on locust wings

characteristic: title


text: Justus-Liebig University of Giessen

text: live

text: Generic

text: Myzus persicae BTI line, parthenogenetic virginoparae apterae

text: Myzus persicae

text: BTI

text: Myzus persicae BTI line SSH cDNA


text: Wallon Agricultural Research Centre

text: live

text: Generic

text: SSH library.

text: 6 days after pollination

characteristic: dev-stage

text: Phaseolus coccineus

text: seed

characteristic: tissue-type

text: cDNA library from Phaseolus coccineus seed


text: Southern Crop Protection and Food Research Centre, Agriculture and Agri-Food Canada

text: live

text: Generic

characteristic: cultivar

text: 29 mg (stage IV, cotyledon) (average seed weight)

text: seed

characteristic: organ

text: Phaseolus vulgaris


text: live

text: Generic

text: 48 mg (stage IV, cotyledon) (average seed weight)

text: seed

text: Phaseolus vulgaris


text: live

text: Generic

text: 164 mg (stage V, cotyledon) (average seed weight)

text: seed

text: Phaseolus vulgaris


text: live

text: Generic

text: 380 mg (stage VIII, maturation) (average seed weight)

text: seed

text: Phaseolus vulgaris


text: Departament Biology, Faculty Chemistry and Biology, University of Santiago

text: live

text: Generic

text: Amplified cDNA were prepared by Creator SMART cDNA library construction kit and normalized by Trimmer-direct kit. Vector was SfiI-digested and desphosphorylated.

text: Spleen

text: Salmo salar

text: Chilean

text: RVAS

text: pDNR-LIB


text: Ateneo de Manila University School of Science and Engineering

text: live

text: Generic

text: E. coli

characteristic: lab-host

text: Eucheuma denticulatum

text: Green variety

text: Thallus

text: E. denticulatum Aspilla et al. 2010

text: Plasmid

text: pDNR-Lib (Clontech)


characteristic: External Id

text: University of Veterinary Medicine Hannover

text: live

text: Generic

text: The subtracted library contains ESTs from preadult (L5) larvae. Subtraction was achieved using the Subtractive Suppression Hybridization (SSH) technique. Differentially expressed sequences were verified by differential screening and Virtual Northern blot using freshly isolated mRNA.

text: preadult fifth stage larvae (L5)

text: Dictyocaulus viviparus

text: whole worms

text: D. viviparus L5-L5hyp subtracted library

text: Plasmid


text: live

text: Generic

text: The subtracted library contains ESTs from hypobiotic preadult (L5hyp) larvae. Subtraction was achieved using the Subtractive Suppression Hybridization (SSH) technique. Differentially expressed sequences were verified by differential screening and Virtual Northern blot using freshly isolated mRNA.

text: hypobiotic preadult fifth stage larvae (L5hyp)

text: Dictyocaulus viviparus

text: whole worms

text: D. viviparus L5hyp-L5 subtracted library

text: Plasmid

characteristic: vector


text: Brigham Young University

characteristic: INSDC center name

text: live

text: Generic

text: Library was prepared by Suppression Subtractive Hybridization with PCR-Select cDNA Subtraction Kit (Clontech)

text: adult

text: Xenopsylla cheopis

text: whole flea

text: oriental rat flea 1 day post-hemoceol injection library


text: Brigham Young University

characteristic: INSDC first public

text: live

text: Generic

text: adult

text: Xenopsylla cheopis

text: whole flea

text: oriental rat flea 1 day post-oral infection library


text: Brigham Young University

characteristic: INSDC last update

text: live

text: Generic

text: adult

text: Xenopsylla cheopis

text: whole flea

text: oriental rat flea 2 days post-oral infection library


text: NingBo University

text: live

characteristic: INSDC status

text: Generic

text: Neobenedenia melleni

text: hermaphrodite

characteristic: sex

text: Neobenedenia melleni eggs, oncomiracidia, immature worms and adults cDNA library


text: Helmholtz Zentrum munich

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Fagus sylvatica

text: leaves

text: Fagus sylvatica leaves


text: Agricultural Genetic Engineering Research Institute

text: live

text: Generic

text: Beta vulgaris

characteristic: organism

text: Beta vulgaris library (El-Khishin DA)

text: pGEM T-Easy


text: live

text: Generic

text: Vicia monantha

text: Vicia monantha library (El-Khishin DA)

text: pGEM T-Easy


text: Universidade Federal de Santa Catarina

text: live

text: Generic

text: proembryos

characteristic: cell type

text: embryonic

text: Araucaria angustifolia

text: Embryogenic cell culture


text: University of Georgia

text: live

text: Generic

text: Total RNAs were isolated from 2 weeks roots of Arachis duranensis with Trizol(Invitrogen). cDNA library was constructed with Smart cDNA Library Construction Kit (Clontech). The inserts were directionally cloned into the Clontech pDNR-LIB vector after SrfI digestion.The plasmids were transformed to the host cell DH10B and selected by antibiotic chloramphenicol.

characteristic: description

text: 2 weeks roots

text: Arachis duranensis

text: SrfI

text: SrfI

text: DUR25, PI.475887

characteristic: strain

characteristic: title

text: (Clontech) pDNR-LIB


text: University of Georgia

text: live

text: Generic

text: Total RNAs were isolated from 6-8 weeks developing seeds of Arachis duranensis with Trizol(Invitrogen). cDNA library was constructed with Smart cDNA Library Construction Kit (Clontech). The inserts were directionally cloned into the Clontech pDNR-LIB vector after SrfI digestion. The plasmids were transformed to the host cell DH10B and selected by antibiotic chloramphenicol.

text: 6-8 weeks developing seeds

text: Arachis duranensis

text: SrfI

text: SrfI

text: DUR25, PI.475887

text: (Clontech) pDNR-LIB


text: University of Georgia

text: live

text: Generic

text: Total RNAs were isolated from 2 weeks roots of Arachis ipaensis with Trizol (Invitrogen). cDNA library was constructed with Smart cDNA Library Construction Kit (Clontech). The inserts were directionally cloned into the Clontech pDNR-LIB vector after SrfI digestion. The plasmids were transformed to the host cell DH10B and selected by antibiotic chloramphenicol.

text: 2 weeks roots

text: Arachis ipaensis

text: SrfI

text: SrfI

text: (Clontech) pDNR-LIB


text: University of Georgia

text: live

text: Generic

text: Total RNAs were isolated from 6-8 weeks developing seeds of Arachis ipaensis with Trizol(Invitrogen). cDNA library was constructed with Smart cDNA Library Construction Kit (Clontech). The inserts were directionally cloned into the Clontech pDNR-LIB vector after SrfI digestion. The plasmids were transformed to the host cell DH10B and selected by antibiotic chloramphenicol.

text: 6-8 weeks developing seeds

text: Arachis ipaensis

text: SrfI

text: SrfI

text: (Clontech) pDNR-LIB


text: UAS, GKVK, Bangalore-560065

text: live

text: Generic

text: Total RNA was isolated from 4-week-old healthy Nicotiana benthamiana leaves and reverse transcribed. Gene specific primer was used for PCR amplification and the amplified product was cloned into T/A cloning vector (pTZ57R/T).

text: 4 wk old leaves

characteristic: dev-stage

text: Escherichia coli

text: Leaf

text: Nicotiana benthamiana

text: Hind III

text: Leaf tissue

characteristic: tissue-type

text: Complementary DNA of Nicotiana benthamiana, 4 wk old leaves


text: UAS, GKVK, Bangalore-560065

text: live

text: Generic

text: Total RNA was isolated from 3-week-old healthy Nicotiana benthamiana leaves and reverse transcribed using OligodT primers. Gene specific primer was used for PCR amplification using cDNA as template. The amplified product was cloned into T/A cloning vector (pTZ57R/T).

text: 3 wk old leaves

text: Escherichia coli

characteristic: lab-host

text: Leaf

characteristic: organ

text: Nicotiana benthamiana

text: Hind III

text: Leaf tissue

text: Complementary DNA of Nicotiana benthamiana, 3 wk old leaves


characteristic: External Id

text: National Center for Genetic Engineering and Biotechnology

text: live

text: Generic

text: Testis of broodstock cultured shrimp

text: E.coli XL-1 Blue MRF

text: testis

text: Penaeus monodon

text: male

text: testis

text: Penaeus monodon Testes Library

text: UNIZAP XR

characteristic: vector


text: National Institute of Plant Genome Research

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: Mycelium

text: Suppression Subtractive Hybridization

characteristic: isolate

text: Ascochyta rabiei

text: Ascochyta rabiei EST library


text: National Center for Genetic Engineering and Biotechnology (BIOTEC)

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: The cDNA library was generated as part of Penaeus monodon EST project. Total RNA was isolated from antennal gland of Penaeus monodon. The mRNA was further purified, reverse transcribed and cloned to Uni-ZAP XR. The cDNA libra

text: E.coli SOLR strain

text: Penaeus monodon

text: EcoR I

text: Xho I

text: Antennal gland

text: Antennal gland-normalized

text: Uni-ZAP XR


text: Chulalongkorn University

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: The cDNA library was generated as part of Penaeus monodon EST project. Total RNA was isolated from gill of juvenile cultured shrimp maintained under 3 ppt salinity for 96 h, 1, 2 and 4 weeks.

text: E.coli SOLR strain

text: Penaeus monodon

text: EcoR I

text: Xho I

text: Gill

text: Gill-salt stress 2-mtDNA subtracted library

text: Uni-ZAP XR


text: Chulalongkorn University

text: live

text: Generic

text: The cDNA library was generated as part of Penaeus monodon EST project. Total RNA was isolated from gill and epipodite of Penaeus monodon. The mRNA was further purified, reverse transcribed and cloned to Uni-ZAP XR.

text: E.coli SOLR strain

text: Penaeus monodon

characteristic: organism

text: EcoR I

text: Xho I

text: Gill and Epipodite

text: Gill-Epipodite library

text: Uni-ZAP XR


text: Chulalongkorn University

text: live

text: Generic

text: The cDNA library was generated as part of Penaeus monodon EST project. Total RNA was isolated from intestine of Penaeus monodon. The mRNA was further purified, reverse transcribed and cloned to Uni-ZAP XR.

characteristic: description

text: E.coli SOLR strain

text: Penaeus monodon

text: EcoR I

text: Xho I

text: Intestine

text: Intestine library

text: Uni-ZAP XR


text: Yildiz Technical University

text: live

text: Generic

text: CloneMiner cDNA Library Construction Kit was used.

text: prenatal

text: Ovis aries

text: Kivircik

characteristic: strain

text: mammary gland

text: cDNA library from sheep prenatal mammary gland

text: Plasmid


text: Yildiz Technical University

text: live

text: Generic

text: postnatal

text: Ovis aries

text: Kivircik

text: mammary gland

text: cDNA library from sheep postnatal mammary gland

characteristic: title

text: Plasmid


text: University of Copenhagen

text: live

text: Generic

text: Derived from several G-type plants

text: Leaves number 3 to 7

characteristic: dev-stage

text: Barbarea vulgaris subsp. arcuata

text: G-type from Herlev (Denmark)

text: Leaves

text: Winter cress G-type


text: Inner Mongolia Agricultural University Huhhot, China

text: live

text: Generic

text: Caragana korshinskii

text: Caragana korshinskii SSH library


text: Chinese Culture University

text: live

text: Generic

characteristic: cultivar

text: 35 day cuttage

text: Ipomoea batatas

text: leaf

characteristic: tissue-type

text: Ipomoea batatas cv. TN71 leaf SSH


text: National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency

text: live

text: Generic

text: The cDNA library was generated as part of Penaeus monodon EST project.

text: Penaeus monodon

text: Gill of juvenile shrimp cultured in normal and heat stress condition (treated with 34.5-36 C seawater for 4 h)

text: Gill-subtractive 1

text: pGEM-T


text: Faculdade de Medicina de Ribeirio Preto da Universidade de Sao Paulo

text: live

text: Generic

text: uncultured microorganisms from human skin.

text: uncultured microorganism

text: uncultured microorganism SSH Library NMMR12

text: Plasmid

text: pGEM-T


text: live

text: Generic

text: uncultured microorganism

text: uncultured microorganism SSH Library NMMR13

text: Plasmid

text: pGEM-T


text: Universidade Federal de Uberlandia

text: live

text: Generic

text: forage

text: venom gland

characteristic: organ

text: Melipona scutellaris

text: female

characteristic: sex

text: Fat body

text: Bee fat body transcriptome

text: Plasmid

text: pCMV.SPORT 6


characteristic: External Id

text: Latvian Biomedical Research and Study Centre

text: live

text: Generic

text: Homo sapiens

text: male

text: testis

text: T7-Testis cDNA

text: Phage

text: T7Select 10-3b Phage Display vector

characteristic: vector


text: Instituto de Investigacion en Recursos Cinegeticos IREC (CSIC-UCLM-JCCM)

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: Total RNA was extracted from 1500 whole partially fed adult female horn flies using the RNAeasy kit (Qiagen, Valencia, CA, USA). The cDNA library was synthesized using the SMART cDNA Library Construction Kit (Clontech, Mountain View, CA, USA) at Creative Biolabs (Port Jefferson Station, NY, USA; http://www.creativebiolabs.com). cDNAs were cloned into the pBluescript II SK vector (Agilent Technologies, Inc., Santa Clara, CA, USA). The library had more than 1x106 primary clones, with >90% recombinants with inserts >500 bp (average cDNA length >1,000 bp).

text: unfed adults

text: Haematobia irritans

text: female

text: UAT colony

text: whole flies

text: Partially fed adult female Haematobia irritans fly cDNA Library

text: pBluescript II SK


text: Faculty of Science, Taif University

characteristic: INSDC last update

text: live

text: Generic

text: Chinese spring

text: Total RNA was isolated from wheat tissues (leaves, anthers, ovaries) before anathesis and used for synthesis of differential expressed fragments among these tissues. Fragments that showed differential expression were recovered from gells, amplified, and cloned in pGM-T vector. Cloned cDNAs were sequenced with T7 or SP6 standard primers.

text: preanathesis

text: Triticum aestivum

text: Anthers

text: Anther specific differential display fragments

text: pGM-T


text: China Agricultural University

text: live

characteristic: INSDC status

text: Generic

text: Four cDNA libraries were constructed using the SSH technique. The first SSH library was made using pooled mRNA (2 microgram) extracted from worms exposed to Escherichia coli O157:H7 against control unexposed, whereas mRNA (2 microgram) from control unexposed worms against worms exposed to Escherichia coli O157:H7 for the second library at 3 days. The third SSH library was made using pooled mRNA (2 microgram) extracted from worms exposed to Escherichia coli O157:H7 against control unexposed, whereas mRNA (2 microgram) from control unexposed worms against worms exposed to Escherichia coli O157:H7 for the fourth library at 30 days. Briefly, the substrate was composed (percentages refer to dry weight) of 10 percent sphagnum, 20 percent kaolinite clay, and 70 percent quartz sand. The pH was adjusted to 6.0 with CaCO3. They were bred at an ambient temperature, and moisture content was maintained around 60 percent Food was first provided with 0.5 percent air-dried cow manure and provided every 14 days for the longest exposure periods; 107CFU g-1 soil (Colony Forming Units per gram of dry matter) of O157:H7 was added to the soil within half an hour of introducing the worms. For the SSH, three groups of 25 adult worms were used per condition (75 worms per condition) and exposure lasted either 3 or 30 days. At the end of the Escherichia coli O157:H7 exposured, groups of 25 earthworms were removed quickly, snap frozen in liquid N2. Total RNA was extracted from the collected whole worm samples using TRIzol Reagent (Invitrogen, CA, USA) according to the manufacturer's protocol. After deoxyribonuclease I treatment, the quantification and purity of RNA samples were assessed using ultraviolet spectrophotometry; the integrity was verified by electrophoresis on 1 percent agarose gels stained with ethidium bromide. 250 microgram of total RNA from each group were used to prepare 2 microgram of mRNA (exposure and control). mRNA were isolated from total RNA using Oligotex mRNA Mini Kit (Qiagen, Hilden, Germany). The Clontech PCR-Select cDNA subtraction kit (BD Biosciences) was then used to enrich for differentially expressed genes.

text: adults

text: Eisenia fetida

text: whole worm

text: Earthworm SSH cDNA library (Wang Xing)


text: University of Valencia Faculty of Biology

text: live

text: Generic

characteristic: NCBI submission model

text: The library contains ESTs obtained from different Suppression Subtractive Hybridizations (SSH) prepared with the midgut of 5th instar larvae from Spodoptera exigua. The different conditions/samples used in the subtractions were: i) Larvae exposed to Bacillus thuringiensis Cry1Ca toxin, ii) Unexposed larvae and iii) Larvae from a Cry1Ca resistant colony. The SSH library was constructed using the Clontech PCR-select cDNA subtraction kit according to the protocols provided by the supplier.

text: 5th instar larvae

text: Spodoptera exigua

text: midgut

text: Sex_SSH Library


text: King Khalid University

text: live

text: Generic

characteristic: NCBI submission package

text: Mus musculus

text: BALB/c

text: Mus musculus BALB/c liver regeneration


text: Central Institute of Medicinal and Aromatic Plants (CIMAP, CSIR)

text: live

text: Generic

text: PCR-based suppression subtractive hybridization was carried out using the cDNA populations derived from the fruit (tester) and leaf (driver) of Piper longum and the subtracted cDNA population was cloned on pGEM-T Easy vector.

text: Fruit

text: Piper longum

characteristic: organism

text: Suppression subtractive hybridization-derived Piper longum fruit cDNA library

text: Plasmid

text: pGEM-T Easy


text: live

text: Generic

text: PCR-based suppression subtractive hybridization was carried out using the cDNA populations derived from the root tuber (tester) and stem + leaf (driver) of Asparagus racemosus and the subtracted cDNA population was cloned on pGEM-T Easy vector.

characteristic: description

text: Root tuber

text: Asparagus racemosus

text: Suppression subtractive hybridization-derived Asparagus racemosus root tuber cDNA library

text: Plasmid

text: pGEM-T Easy


text: live

text: Generic

text: PCR-based suppression subtractive hybridization was carried out using the cDNA populations derived from the root (tester) and leaf (driver) of Vetiveria zizanioides and the subtracted cDNA population was cloned on pGEM-T Easy vector.

text: Root

text: Chrysopogon zizanioides

text: Suppression subtractive hybridization-derived Vetiveria zizanioides root cDNA library

text: Plasmid

text: pGEM-T Easy


text: live

text: Generic

text: PCR-based suppression subtractive hybridization was carried out using the cDNA populations derived from the leaf (tester) and root (driver) of Cynodon dactylon and the subtracted cDNA population was cloned on pGEM-T Easy vector.

text: Leaf

text: Cynodon dactylon

text: Suppression subtractive hybridization-derived Cynodon dactylon leaf cDNA library

text: Plasmid

text: pGEM-T Easy


text: live

text: Generic

text: PCR-based suppression subtractive hybridization was carried out using the cDNA populations derived from the root bark (tester) and leaf (driver) of Plumbago zeylanica and the subtracted cDNA population was cloned on pGEM-T Easy vector.

text: Root bark

text: Plumbago zeylanica

text: Suppression subtractive hybridization-derived Plumbago zeylanica root bark cDNA library

text: Plasmid

text: pGEM-T Easy


text: live

text: Generic

text: Ocimum tenuiflorum: synonym = Ocimum sanctum. PCR-based suppression subtractive hybridization was carried out using the cDNA populations derived from the leaf (tester) and root + stem (driver) of Ocimum sanctum and the subtracted cDNA population was cloned on pGEM-T Easy vector.

text: Leaf

text: Ocimum tenuiflorum

text: Suppression subtractive hybridization-derived Ocimum sanctum leaf cDNA library

text: Plasmid

text: pGEM-T Easy


text: live

text: Generic

text: PCR-based suppression subtractive hybridization was carried out using the cDNA populations derived from the rhizome (tester) and leaf (driver) of Acorus calamus and the subtracted cDNA population was cloned on pGEM-T Easy vector.

text: Rhizome

text: Acorus calamus

text: Suppression subtractive hybridization-derived Acorus calamus rhizome cDNA library

text: Plasmid

text: pGEM-T Easy


text: live

text: Generic

text: PCR-based suppression subtractive hybridization was carried out using the cDNA populations derived from the rhizome (tester) and leaf (driver) of Curcuma longa and the subtracted cDNA population was cloned on pGEM-T Easy vector.

text: Rhizome

text: Curcuma longa

text: Suppression subtractive hybridization-derived Curcuma longa rhizome cDNA library

text: Plasmid

text: pGEM-T Easy


text: Central Institute of Medicinal and Aromatic Plants (CIMAP, CSIR)

text: live

text: Generic

text: PCR-based suppression subtractive hybridization was carried out using the cDNA populations derived from the root (tester) and leaf (driver) of Sida cordifolia and the subtracted cDNA population was cloned on pGEM-T Easy vector.

text: Root

text: Sida cordifolia

text: Suppression subtractive hybridization-derived Sida cordifolia root cDNA library

text: Plasmid

text: pGEM-T Easy


text: live

text: Generic

text: PCR-based suppression subtractive hybridization was carried out using the cDNA populations derived from the stem (tester) and leaf (driver) of Commiphora wightii and the subtracted cDNA population was cloned on pGEM-T Easy vector.

text: Stem

text: Commiphora wightii

text: Suppression subtractive hybridization-derived Commiphora wightii stem cDNA library

text: Plasmid

text: pGEM-T Easy


text: live

text: Generic

text: PCR-based suppression subtractive hybridization was carried out using the cDNA populations derived from the root (tester) and leaf (driver) of Ricinus communis and the subtracted cDNA population was cloned on pGEM-T Easy vector.

text: Root

text: Ricinus communis

text: Suppression subtractive hybridization-derived Ricinus communis root cDNA library

text: Plasmid

text: pGEM-T Easy


text: live

text: Generic

text: PCR-based suppression subtractive hybridization was carried out using the cDNA populations derived from the stem (tester) and flower + leaf (driver) of Santalum album and the subtracted cDNA population was cloned on pGEM-T Easy vector.

text: Stem

text: Santalum album

text: Suppression subtractive hybridization-derived Santalum album stem cDNA library

text: Plasmid

text: pGEM-T Easy


text: Universidade Federal do Parana - UFPR

text: live

text: Generic

text: adult

text: venomous gland

text: Loxosceles intermedia

text: Loxosceles intermedia venomous gland library

text: pDNR-LIB


text: The Chinese University of Hong Kong

text: live

text: Generic

text: Lentinula edodes

characteristic: strain

text: mature fruiting body before and during sporulation

text: Lentinula edodes mature fruiting body EST contigs


text: Nanhua University

text: live

text: Generic

characteristic: cell-line

text: PCR based suppression subtractive hybridization was carried out using the cDNA populations derived from the DADS-treated and untreated Caco-2 cells taken as 'tester' and 'driver', respectively.

text: 44 years adult

text: Homo sapiens

text: female

text: colorectal adenocarcinoma

text: cDNA library of up-regulated genes induced by DADS in HT-29 cells

characteristic: title

text: pGEM-T


text: Nanhua University

text: live

text: Generic

text: PCR based suppression subtractive hybridization was carried out using the cDNA populations derived from the DADS-treated and untreated Caco-2 cells taken as 'driver' and 'tester', respectively.

text: 44 years adult

characteristic: dev-stage

text: Homo sapiens

text: female

text: colorectal adenocarcinoma

text: cDNA library of down-regulated genes induced by DADS in HT-29 cells

text: pGEM-T


text: CIRAD Departement Systemes Biologiques

text: live

text: Generic

characteristic: cultivar

text: SSH library with wounding ethylene treatment on the bark tissue in clone PB260 hevea brasiliensis

text: Hevea brasiliensis

text: bark

characteristic: tissue-type

text: SSH Library of rubber tree


text: Universite Politecnica delle Marche

text: live

text: Generic

text: Solea solea

text: liver

text: Solea solea library


text: Nanjing Normal University

text: live

text: Generic

text: the sequence come from cDNA RDA between deltamethrin strain and susceptible strain,using HhaI as restriction enzyme.

text: adult moth

text: Plutella xylostella

text: susceptible strain

text: differential expressed sequence of susceptible diamondback moth


text: Nanjing Normal University

text: live

text: Generic

text: the sequence come from cDNA RDA between deltamethrin strain and susceptible strain,using HhaI asrestriction enzyme.

text: adult moth

text: Plutella xylostella

text: deltamethrin strain

text: differential expressed sequence of deltamethrin diamondback moth


text: Horticultural Institute, Zhejiang Academy of Agricultural Sciences

text: live

text: Generic

text: Amakusa

text: Gene expression changes in response to drought stress were studied in citrus (Citrus sinensis x Citrus reticulata cv. Amakusa). cDNA sequences of differentially expressed TDFs were isolated and analyzed.

text: Citrus sinensis x Citrus reticulata

text: cDNA fragments of drought-stress-induced genes from 'Amakusa' tangor


text: Oil Crops Research Institute

text: live

text: Generic

text: immature seed

text: Glycine max

text: Zhongdou 32

text: Soybean immature seed full-length-enriched cDNA library


text: IDR Universidad Castilla-La Mancha

text: live

text: Generic

text: suppresive hybridization performed with Clontech PCR-Select cDNA Subtraction kit

text: Stigma

characteristic: organ

text: Crocus sativus

text: EcoRI

text: EcoRI

text: Suppresive hybridization library from immature/mature stigmas

text: plasmid

text: pGEMT-Easy


characteristic: External Id

text: Katholieke Universiteit Leuven, Faculty of sciences, Department Biology

text: live

text: Generic

text: Daphnia magna

text: female

characteristic: sex

text: 48 and 96h exposures to Pasteuria spores


characteristic: INSDC center name

text: live

text: Generic

text: 5,6 and 8ug/l carbaryl was used.

text: Daphnia magna

text: female

text: 48, 96 and 144h exposures to carbaryl


characteristic: INSDC first public

text: live

text: Generic

text: Daphnia magna

text: female

text: 48 and 96h exposures to fish kairomones


text: National Research Center on Plant Biotechnology

characteristic: INSDC last update

text: live

text: Generic

text: Vaniglia Sanguigno

text: SSH libraries prepared from excised ovules collected from pre and post anthesis stages of Citrus sinensis cv. Vaniglia Sanguigno. RNA was isolated from ovules using Trizol method of RNA isolation. SSH was carried both in forward and reverse direction to detect the genes specifically expresses during polyembryonic initials. Clontech PCR-Select cDNA Subtraction kit (Cat. No. 637401) was used to make the libraries. All the sequences were generated as single pass reads, using T7 universal promoter sequence as primer. Sequences submitted are trimmed from low quality sequences and pGEMT-Easy vector backbone. These are the sequences from forward library which includes Tester as post anthesis ovules and Driver as pre anthesis ovules.

text: Post anthesis

text: Ovule

text: Citrus sinensis

text: Library from post anthesis stages of ovules of polyembryonic Citrus sinensis

text: Plasmid

text: pGEMT-Easy

characteristic: vector


text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Vaniglia Sanguigno

text: SSH libraries prepared from excised ovules collected from pre and post anthesis stages of Citrus sinensis cv. Vaniglia Sanguigno. RNA was isolated from ovules using Trizol method of RNA isolation. SSH was carried both in forward and reverse direction to detect the genes specifically expresses during polyembryonic initials. Clontech PCR-Select cDNA Subtraction kit (cat. No.637401) was used to make the libraries. All the sequences were generated as single pass reads, using T7 universal promoter sequence as primer. Sequences submitted are trimmed from low quality sequences and pGEMT-Easy vector backbone. These are the sequences from reverse library which includes Tester as pre anthesis ovules and Driver as post anthesis ovules.

text: pre anthesis

text: Ovule

text: Citrus sinensis

text: Library from pre anthesis stages of ovules of polyembryonic Citrus sinensis

text: Plasmid

text: pGEMT-Easy


text: Federal University of Rio Grande do Sul

text: live

text: Generic

characteristic: NCBI submission package

text: Differentiating xylem was derived from 4-year-old Eucalyptus grandis trees located in commercial plantations of Veracel Celulose S.A. (Eunapolis, BA, Brazil). Xylem was collected by scraping the exposed vascular tissue after the removal of the 0.5-1 cm-tick stem bark. The biological material was wrapped in aluminum foil and immediately frozen in liquid nitrogen. Total RNA was extracted from 100-200 mg of tissue previously pulverized in mortars with pestles and liquid nitrogen using the PureLink Plant RNA Reagent Kit (Invitrogen) according to the multi-miniprep protocol instructions of the manufacturer. Polyadenylated RNA was subsequently purified from total RNA using the Oligotex mRNA Mini kit (Qiagen). RNA purity and quality were checked by both agarose gel electrophoresis and spectrophotometric measurements at 260 and 280 nm (Ausubel et al., 2003) (Ausubel et al., 2003). Complementary DNA libraries were constructed using 1-5 ug mRNA and kits of the Superscript Plasmid System with Gateway Technology for cDNA Synthesis and Cloning (Invitrogen) and instructions of the manufacturer. Eluted fractions of double-stranded cDNA were stored at -20 oC and further ligated into pSport1 (Invitrogen). Ligated products were transformed into Escherichia coli Electromax DH10B competent cells (Invitrogen) using a Gene Pulser II Electroporation System (Bio-Rad) and 0.2 mm cuvettes. Aliquots of transformed cells were strayed with Drigalsky loops on 16 cm large Petri dishes containing CircleGrow medium (Qbiogene) solidified by 1.5 percent agar (GE Healthcare) and containing 110 mg/L ampicillin (Invitrogen), 20 mg/L X-Gal (Invitrogen) and 100 uM IPTG (Invitrogen). White and faint-blue colonies were inoculated in 1 mL CircleGrow containing 110 mg/L ampicillin distributed in 96-deep-well plates (2.2 mL). Bacteria were grown for 18 h in shakers at 37 oC and 280 rpm. Recombinant pSport1 plasmids were purified via the alcaline lysis protocol and filtration through 96-well glass-fiber plates (Millipore). Sanger automated sequencing was carried out in automatic sequencers model ABI Prism 3100 Genetic Analyzer (Applied Biosystems) armed with 50 cm capillaries and POP6 polymer (Applied Biosystems). DNA templates (30 to 45 ng) were labeled with 3.2 pmol pSport1-derived primer 5'-ATAGGGAAAGCTGGTACGC-3' (GlyptsRev-1 primer) and 1-2 uL of BigDye Terminator v3.1 Cycle Sequencing (Applied Biosystems) in a final volume of 10 uL. Labeling reactions were performed in GeneAmp PCR System 9700 termocyclers (Applied Biosystems) with a initial denaturing step of 96 oC for 3 min followed by 25 cycles of 96 oC for 10 s, 55 oC for 5 s and 60 oC for 4 min. Labeled samples were purified by isopropanol precipitation followed by 70 percent ethanol rinsing. Precipitated products were suspended in 10 uL Hi-Di Formamide (Applied Biosystems), denatured at 95 oC for 5 min, ice-cooled for 5 min and electroinjected in the automatic sequencers. Sequencing data were collected using the software Data Collection v1.0.1 (Applied Biosystems) programmed with the following parameters: Dye Set 'Z'; Mobility File 'DT3100POP6{BDv3}v1.mob'; BioLIMS Project '3100_Project1'; Run Module 1 'StdSeq50_POP6_50cm_cfv_100'; and Analysis Module 1 'BC-3100SR_Seq_FASTA.saz'. Raw sequence reads generated were submmitted to pre-processing steps, namely (i) base-calling using the program Phred; (ii) sequence quality trimming (LUCY); and (iii) vector masking (Cross_Match; Ewing, personal communication). A quality control filter was applied, and sequences were retained if having lengths of at least 250 bp with 1 percent base-calling error probability (Phred score = 20).

text: Field-grown adult trees (4 years-old)

text: Escherichia coli DH10B

characteristic: lab-host

text: Stem

text: Eucalyptus grandis

characteristic: organism

text: SalI

text: NotI

text: Hermaphrodite (perfect flowers)

text: Differentiating xylem

text: Eucalyptus grandis Xylem (XY) vascular tissue

text: Plasmid

text: pSport1 (Invitrogen)


text: Federal University of Rio Grande do Sul

text: live

text: Generic

text: Surface-sterilized seeds from E. grandis were germinated in vitro in 1/4 Murashige & Skoog medium (MS, Invitrogen) solidified by 0.7 percent (w/v) Phytoagar (Duchefa) at 26 oC and a photoperiod of 16 h. Seedlings were harvested 2 months after germination, wrapped in aluminum foil and immediately frozen in liquid nitrogen. Total RNA was extracted from 100-200 mg of tissue previously pulverized in mortars with pestles and liquid nitrogen using the PureLink Plant RNA Reagent Kit (Invitrogen) according to the multi-miniprep protocol instructions of the manufacturer. Polyadenylated RNA was subsequently purified from total RNA using the Oligotex mRNA Mini kit (Qiagen). RNA purity and quality were checked by both agarose gel electrophoresis and spectrophotometric measurements at 260 and 280 nm (Ausubel et al., 2003) (Ausubel et al., 2003). Complementary DNA libraries were constructed using 1-5 ug mRNA and kits of the Superscript Plasmid System with Gateway Technology for cDNA Synthesis and Cloning (Invitrogen) and instructions of the manufacturer. Eluted fractions of double-stranded cDNA were stored at -20 oC and further ligated into pSport1 (Invitrogen). Ligated products were transformed into Escherichia coli Electromax DH10B competent cells (Invitrogen) using a Gene Pulser II Electroporation System (Bio-Rad) and 0.2 mm cuvettes. Aliquots of transformed cells were strayed with Drigalsky loops on 16 cm large Petri dishes containing CircleGrow medium (Qbiogene) solidified by 1.5 percent agar (GE Healthcare) and containing 110 mg/L ampicillin (Invitrogen), 20 mg/L X-Gal (Invitrogen) and 100 uM IPTG (Invitrogen). White and faint-blue colonies were inoculated in 1 mL CircleGrow containing 110 mg/L ampicillin distributed in 96-deep-well plates (2.2 mL). Bacteria were grown for 18 h in shakers at 37 oC and 280 rpm. Recombinant pSport1 plasmids were purified via the alcaline lysis protocol and filtration through 96-well glass-fiber plates (Millipore). Sanger automated sequencing was carried out in automatic sequencers model ABI Prism 3100 Genetic Analyzer (Applied Biosystems) armed with 50 cm capillaries and POP6 polymer (Applied Biosystems). DNA templates (30 to 45 ng) were labeled with 3.2 pmol pSport1-derived primer 5'-ATAGGGAAAGCTGGTACGC-3' (GlyptsRev-1 primer) and 1-2 uL of BigDye Terminator v3.1 Cycle Sequencing (Applied Biosystems) in a final volume of 10 uL. Labeling reactions were performed in GeneAmp PCR System 9700 termocyclers (Applied Biosystems) with a initial denaturing step of 96 oC for 3 min followed by 25 cycles of 96 oC for 10 s, 55 oC for 5 s and 60 oC for 4 min. Labeled samples were purified by isopropanol precipitation followed by 70 percent ethanol rinsing. Precipitated products were suspended in 10 uL Hi-Di Formamide (Applied Biosystems), denatured at 95 oC for 5 min, ice-cooled for 5 min and electroinjected in the automatic sequencers. Sequencing data were collected using the software Data Collection v1.0.1 (Applied Biosystems) programmed with the following parameters: Dye Set 'Z'; Mobility File 'DT3100POP6{BDv3}v1.mob'; BioLIMS Project '3100_Project1'; Run Module 1 'StdSeq50_POP6_50cm_cfv_100'; and Analysis Module 1 'BC-3100SR_Seq_FASTA.saz'. Raw sequence reads generated were submmitted to pre-processing steps, namely (i) base-calling using the program Phred; (ii) sequence quality trimming (LUCY); and (iii) vector masking (Cross_Match; Ewing, personal communication). A quality control filter was applied, and sequences were retained if having lengths of at least 250 bp with 1 percent base-calling error probability (Phred score = 20).

characteristic: description

text: In vitro-grown, 2 month-old seedlings

text: Escherichia coli DH10B

text: Whole plant (roots, stems and leaves)

text: Eucalyptus grandis

text: SalI

text: NotI

text: Hermaphrodite (perfect flowers)

text: Eucalyptus grandis Whole seedlings (untreated)

text: Plasmid

text: pSport1 (Invitrogen)


text: Federal University of Rio Grande do Sul

text: live

text: Generic

text: Surface-sterilized seeds from E. grandis were germinated in vitro in 1/4 Murashige & Skoog medium (MS, Invitrogen) solidified by 0.7 percent (w/v) Phytoagar (Duchefa) at 26 oC and a photoperiod of 16 h. Two months after germination, groups of 3-4 plantlets were separately sprayed three times with 10 mg/L 2,4- dichlorophenoxyacetic acid, 10 mg/L kinetin, 10 mg/L ethephon, 10 mg/L abscisic acid, 10 mg/L gibberellic acid, 1 g/L paclobutrazol, 1 mM salicylic acid, 1 mM methyl jasmonic acid, 1 mM paraquat or water as control. Groups of 3-4 plantlets were sprayed three-times with water and then irradiated with ultraviolet light (354 nm, 40 cm apart) for 4 h or for two periods of 4 h each. Another group of 3-4 plants were water sprayed and kept at 10 oC during 16 h. After the series of treatments, all plants were maintained under normal growth conditions during 24 or 48 h before freezing them in liquid nitrogen. Seedlings were wrapped in aluminum foil and immediately frozen in liquid nitrogen. Total RNA was extracted from 100-200 mg of tissue previously pulverized in mortars with pestles and liquid nitrogen using the PureLink Plant RNA Reagent Kit (Invitrogen) according to the multi-miniprep protocol instructions of the manufacturer. Polyadenylated RNA was subsequently purified from total RNA using the Oligotex mRNA Mini kit (Qiagen). RNA purity and quality were checked by both agarose gel electrophoresis and spectrophotometric measurements at 260 and 280 nm (Ausubel et al., 2003) (Ausubel et al., 2003). Complementary DNA libraries were constructed using 1-5 ug mRNA and kits of the Superscript Plasmid System with Gateway Technology for cDNA Synthesis and Cloning (Invitrogen) and instructions of the manufacturer. Eluted fractions of double-stranded cDNA were stored at -20 oC and further ligated into pSport1 (Invitrogen). Ligated products were transformed into Escherichia coli Electromax DH10B competent cells (Invitrogen) using a Gene Pulser II Electroporation System (Bio-Rad) and 0.2 mm cuvettes. Aliquots of transformed cells were strayed with Drigalsky loops on 16 cm large Petri dishes containing CircleGrow medium (Qbiogene) solidified by 1.5 percent agar (GE Healthcare) and containing 110 mg/L ampicillin (Invitrogen), 20 mg/L X-Gal (Invitrogen) and 100 uM IPTG (Invitrogen). White and faint-blue colonies were inoculated in 1 mL CircleGrow containing 110 mg/L ampicillin distributed in 96-deep-well plates (2.2 mL). Bacteria were grown for 18 h in shakers at 37 oC and 280 rpm. Recombinant pSport1 plasmids were purified via the alcaline lysis protocol and filtration through 96-well glass-fiber plates (Millipore). Sanger automated sequencing was carried out in automatic sequencers model ABI Prism 3100 Genetic Analyzer (Applied Biosystems) armed with 50 cm capillaries and POP6 polymer (Applied Biosystems). DNA templates (30 to 45 ng) were labeled with 3.2 pmol pSport1-derived primer 5'-ATAGGGAAAGCTGGTACGC-3' (GlyptsRev-1 primer) and 1-2 uL of BigDye Terminator v3.1 Cycle Sequencing (Applied Biosystems) in a final volume of 10 uL. Labeling reactions were performed in GeneAmp PCR System 9700 termocyclers (Applied Biosystems) with a initial denaturing step of 96 oC for 3 min followed by 25 cycles of 96 oC for 10 s, 55 oC for 5 s and 60 oC for 4 min. Labeled samples were purified by isopropanol precipitation followed by 70 percent ethanol rinsing. Precipitated products were suspended in 10 uL Hi-Di Formamide (Applied Biosystems), denatured at 95 oC for 5 min, ice-cooled for 5 min and electroinjected in the automatic sequencers. Sequencing data were collected using the software Data Collection v1.0.1 (Applied Biosystems) programmed with the following parameters: Dye Set 'Z'; Mobility File 'DT3100POP6{BDv3}v1.mob'; BioLIMS Project '3100_Project1'; Run Module 1 'StdSeq50_POP6_50cm_cfv_100'; and Analysis Module 1 'BC-3100SR_Seq_FASTA.saz'. Raw sequence reads generated were submmitted to pre-processing steps, namely (i) base-calling using the program Phred; (ii) sequence quality trimming (LUCY); and (iii) vector masking (Cross_Match; Ewing, personal communication). A quality control filter was applied, and sequences were retained if having lengths of at least 250 bp with 1 percent base-calling error probability (Phred score = 20).

text: In vitro-grown, 2 month-old seedlings

text: Escherichia coli DH10B

text: Whole plant (roots, stems and leaves)

text: Eucalyptus grandis

text: SalI

text: NotI

text: Hermaphrodite (perfect flowers)

text: Eucalyptus grandis Whole seedlings (treated)

characteristic: title

text: Plasmid

text: pSport1 (Invitrogen)


text: Federal University of Rio Grande do Sul

text: live

text: Generic

text: Mature, fully-expanded leaves derived from 4-year-old Eucalyptus grandis trees located in commercial plantations of Veracel Celulose S.A. (Eunapolis, BA, Brazil). The biological material was wrapped in aluminum foil and immediately frozen in liquid nitrogen. Total RNA was extracted from 100-200 mg of tissue previously pulverized in mortars with pestles and liquid nitrogen using the PureLink Plant RNA Reagent Kit (Invitrogen) according to the multi-miniprep protocol instructions of the manufacturer. Polyadenylated RNA was subsequently purified from total RNA using the Oligotex mRNA Mini kit (Qiagen). RNA purity and quality were checked by both agarose gel electrophoresis and spectrophotometric measurements at 260 and 280 nm (Ausubel et al., 2003) (Ausubel et al., 2003). Complementary DNA libraries were constructed using 1-5 ug mRNA and kits of the Superscript Plasmid System with Gateway Technology for cDNA Synthesis and Cloning (Invitrogen) and instructions of the manufacturer. Eluted fractions of double-stranded cDNA were stored at -20 oC and further ligated into pSport1 (Invitrogen). Ligated products were transformed into Escherichia coli Electromax DH10B competent cells (Invitrogen) using a Gene Pulser II Electroporation System (Bio-Rad) and 0.2 mm cuvettes. Aliquots of transformed cells were strayed with Drigalsky loops on 16 cm large Petri dishes containing CircleGrow medium (Qbiogene) solidified by 1.5 percent agar (GE Healthcare) and containing 110 mg/L ampicillin (Invitrogen), 20 mg/L X-Gal (Invitrogen) and 100 uM IPTG (Invitrogen). White and faint-blue colonies were inoculated in 1 mL CircleGrow containing 110 mg/L ampicillin distributed in 96-deep-well plates (2.2 mL). Bacteria were grown for 18 h in shakers at 37 oC and 280 rpm. Recombinant pSport1 plasmids were purified via the alcaline lysis protocol and filtration through 96-well glass-fiber plates (Millipore). Sanger automated sequencing was carried out in automatic sequencers model ABI Prism 3100 Genetic Analyzer (Applied Biosystems) armed with 50 cm capillaries and POP6 polymer (Applied Biosystems). DNA templates (30 to 45 ng) were labeled with 3.2 pmol pSport1-derived primer 5'-ATAGGGAAAGCTGGTACGC-3' (GlyptsRev-1 primer) and 1-2 uL of BigDye Terminator v3.1 Cycle Sequencing (Applied Biosystems) in a final volume of 10 uL. Labeling reactions were performed in GeneAmp PCR System 9700 termocyclers (Applied Biosystems) with a initial denaturing step of 96 oC for 3 min followed by 25 cycles of 96 oC for 10 s, 55 oC for 5 s and 60 oC for 4 min. Labeled samples were purified by isopropanol precipitation followed by 70 percent ethanol rinsing. Precipitated products were suspended in 10 uL Hi-Di Formamide (Applied Biosystems), denatured at 95 oC for 5 min, ice-cooled for 5 min and electroinjected in the automatic sequencers. Sequencing data were collected using the software Data Collection v1.0.1 (Applied Biosystems) programmed with the following parameters: Dye Set 'Z'; Mobility File 'DT3100POP6{BDv3}v1.mob'; BioLIMS Project '3100_Project1'; Run Module 1 'StdSeq50_POP6_50cm_cfv_100'; and Analysis Module 1 'BC-3100SR_Seq_FASTA.saz'. Raw sequence reads generated were submmitted to pre-processing steps, namely (i) base-calling using the program Phred; (ii) sequence quality trimming (LUCY); and (iii) vector masking (Cross_Match; Ewing, personal communication). A quality control filter was applied, and sequences were retained if having lengths of at least 250 bp with 1 percent base-calling error probability (Phred score = 20).

text: Fully expanded, mature leaves from field-grown adult trees (4 years-old)

characteristic: dev-stage

text: Escherichia coli DH10B

text: Leaf

text: Eucalyptus grandis

text: SalI

text: NotI

text: Hermaphrodite (perfect flowers)

text: Eucalyptus grandis Mature leaves

text: Plasmid

text: pSport1 (Invitrogen)


text: Nanjing Agricultural University

text: live

text: Generic

text: Chrysanthemum x morifolium

text: flower

text: Chrysanthemum flower cDNA library


text: The University of Tokyo, Laboratory of Insect Genetics and Bioscience

text: live

text: Generic

text: pupa

text: Bombyx mori

text: female

text: ovaries

text: cDNA library from pupal ovaries


text: live

text: Generic

text: pupa

text: Bombyx mori

text: male

text: testis

text: cDNA library from pupal testis


text: Fisheries College, Jimei University

text: live

text: Generic

text: SMART cDNA library.

text: mature

text: pooled organ

text: Scylla paramamosain

text: SfiI A

text: SfiI B

text: Scylla paramamosain pooled organ cDNA library

text: Plasmid

text: pDNR-LIB


text: University of California--Riverside

text: live

text: Generic

text: RNA extracted from whole eggs and tissues was used to construct cDNA libraries from which ESTs were generated.

text: eggs, central nervous system

text: E.coli

text: Heliothis virescens

text: Heliothis virescens eggs, central nervous system cDNA Libraries

text: plasmid


text: Pontificia Universidad Catolica de Chile

text: live

text: Generic

text: Bluegold

characteristic: cultivar

text: cDNA-AFLP was carrying out to identify genes that expressed with aluminum treatment in Vaccinium corymbosum L. The expression was compared between contrasting genotypes.

text: 3 cm from Tips root

text: Vaccinium corymbosum

text: Root

text: Short-term aluminum-stress in root cv. Bluegold cDNA-AFLP


text: live

text: Generic

text: Brigitta

text: 3 cm from Tips root

text: Vaccinium corymbosum

text: Root

characteristic: tissue-type

text: Short-term aluminum-stress in root cv. Brigitta cDNA-AFLP


text: University of Helsinki

text: live

text: Generic

text: Isolated from the Suillus bovinus treated roots of Scots pine by differential display reverse transcription PCR using anchor T20 and random primer OPG7 by comparing with the untreated controls.

text: Pinus sylvestris

text: roots

text: Pinus sylvestris Suillus bovinus treated roots


text: University of Helsinki

text: live

text: Generic

text: Isolated from the untreated control roots of Scots pine by differential display reverse transcription PCR using anchor T20 and random primer OPG7 by comparing with the S uillus bovinus treated samples.

text: Pinus sylvestris

text: roots

text: Pinus sylvestris roots untreated control


text: Oregon State University

text: live

text: Generic

text: The subtraction was performed using five day old larvae collected in August of 1998 and the Clontech PCR-Select cDNA subtraction kit according to the manufacturer's instructions. Starting amounts of 1.45 ug mRNA from a 3-bowl mixture of symbiotic larvae (each with an infection rate of 78%) and 1.24 ug mRNA from a 4-bowl mixture of aposymbiotic larvae were used to construct the libraries. cDNAs were inserted into a T/A cloning vector.

text: larval day 5 post-fertilization

text: Lobactis scutaria

text: whole organism

text: Fungia scutaria larval apo subtracted library


text: Oregon State University

text: live

text: Generic

text: larval day 5 post-fertilization

text: Lobactis scutaria

text: whole organism

text: Fungia scutaria larval apo library


text: Oregon State University

text: live

text: Generic

text: larval day 5 post-fertilization

text: Lobactis scutaria

text: whole organism

text: Fungia scutaria larval sym library


text: Wroclaw University Institute of Plant Biology

text: live

text: Generic

text: These ESTs relate to several genes (isogenes) that encode protein H+-ATPase in cucumber. Specific primers were designed based on contigs of cucumber genome (accession code ACHR00000000) and the sequences were amplified via PCR reaction using cDNA as a template.

text: Cucumis sativus

text: cDNA synthesized from Cucumis sativus mRNA


text: Mubarak City for Scientific Research and Technology Applications

text: live

text: Generic

text: Sakha 101

text: cDNA amplified through the Differential Display (DD-PCR) from the extracted RNA from rice local variety 'Sakha 101'. The plant was treated with sodium arsenate.

text: 9-10 days afer germination

text: Oryza sativa

text: leaves

text: Oryza sativa Sakha 101 treated with sodium arsenate


text: Universidad de Mal

text: live

text: Generic

text: Pinus pinaster

text: root

text: Pinus pinaster root (Avila)


text: Genoscope - Centre National de Sequencage

text: live

text: Generic

text: Yarrowia lipolytica

text: YPD medium in exponential phase


text: live

text: Generic

text: Yarrowia lipolytica

text: YPD medium in stationary phase


text: live

text: Generic

text: Yarrowia lipolytica

text: oleic acid medium in exponential phase


text: live

text: Generic

text: Total RNA was extracted from shock-frozen tissue; mRNA was purified on Ambion's poly(A)Purist columns. cDNAS was synthesised by the oligo-capping method described by Maruyama, K. and Sugano, S. (1994) Gene 138: 171-174 and by Suzuki, Y. et al. (1997) Gene 200: 149-156 in order to enrich full-length cDNAs. The molecules were the inserted into the pDONR222.1 (Invitrogen), cloned and sequenced at the Genoscope, Evry, France by Applied Biosystems 3730 automated sequencers.

text: Rattus norvegicus

text: Sprague-Dawley

characteristic: strain

text: brain

text: Rattus norvegicus brain Sprague-Dawley


characteristic: External Id

text: live

text: Generic

text: Rattus norvegicus

text: Sprague-Dawley

text: liver

text: Rattus norvegicus liver Sprague-Dawley


text: live

text: Generic

text: Rattus norvegicus

text: Sprague-Dawley

text: skeletal muscle

text: Rattus norvegicus skeletal muscle Sprague-Dawley


text: National Institute of Immunology

text: live

text: Generic

text: Bubalus bubalis

text: mammary gland

text: Bubalus bubalis mammary gland


text: Washington University School of Medicine

text: live

text: Generic

text: Libraries prepared by Phil Wigge, Plant Molecular and Cellular Biology, Lab (Detlev Weigel), The Salk Institute for Biological Science, 10010 North Torrey Pines Road, La Jolla, CA 92037.

text: Hydra vulgaris

text: NotI

text: SalI

text: Hydra cDNA library

text: plasmid


text: live

text: Generic

text: The library was constructed with the Invitrogen kit: Superscript Plasmid System with Gateway Technology for DNA Synthesis and Cloning [invitrogen Cat. No. 18248]. The oligo used for first-strand cDNA synthesis produced by Genset was GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTTTTTNN. The bacterial host is DH5. Bacterial colonies were grown on LB-agar plates for arraying. An Autogen automated colony picker was used to array the colonies Arrayed colonies were grown in TYE medium [tryptone, yeast extract, NaCl] plus freezing salts [contains glycerol]. Some of the wells either contain no medium, or no bacteria grew up.

text: Hydra vulgaris

text: NotI

text: SalI

text: Hydra EST -II

text: plasmid


text: live

text: Generic

text: a) 1st strand cDNA was primed with a XhoI oligo (dT) primer (5' ACTAAAGGGCTCGAG(T)18NN)3' ; b) Double-stranded cDNA was ligated to PstI linker, digested with PstI and XhoI and cloned into the PstI and XhoI sites of a PCS22 vector, which is a modified pCS2+ vector. c) The pCS22 vector contains a T7 promoter site [standard from pBluescript] at the 5' end of the cloning site, and a T3 promoter site [standard from pBluescript] at the 3' end of the cloning site. d) The ligation mix was transformed into TOP10F' cells [ = DH10B cells]. e) The cells were grown in SOC = 5g yeast, 20g tryptone, 0.5g NaCl, 10mM MgSO4, 10mM MgCl, 0.2% glucose/Liter, (no antibiotic). f) The frequency of vectors containing inserts is >90% [42 of 42]. g) The average size of the 42 inserts is 1075 +/- 850].

text: Hydra vulgaris

text: XhoI

text: PstI

text: Hydra EST -III

text: plasmid


text: live

text: Generic

text: a) 1st strand cDNA was primed with a XhoI oligo (dT) primer (5' ACTAAAGGGCTCGAG(T)18NN)3' b) Double-stranded cDNA was ligated to PstI linker, digested with PstI and XhoI and cloned into the PstI and XhoI sites of a pBluesript SK- vector. c) The ligation mix was transformed into DH10B cells. d) The cells were grown in SOC = 5g yeast, 20g tryptone, 0.5g NaCl, 10mM MgSO4, 10mM MgCl, 0.2% glucose/Liter, (no antibiotic). e) DMSO was added to a final conc. of 10% as a cryoprotectant and frozen f) The titre before freezing was determined as 4200/100 ul. Assuming a 20% loss upon freezing, the titre is probably ~ 3500/100 ul. g) The frequency of vectors containing inserts is 98% as measured by colony color. 2% were blue [b-galactosidase], 98% were white. h) A low level of 33P was used in the cDNA synthesis procedure. The level measued by holding a Geiger Counter next to a tube was background. i) Preliminary test of library. 20 clones were sequenced from both ends. The T3 primer was used for the 5' end and yielded good sequence for 16/20 clones. The T7 primer was used for the 3' end and yielded good sequence for 12/19 clones. 6/7 of the poor sequences had very long poly A tails. This problem can probably be overcome by using a degenerate T primer for the 3' end.

text: Hydra vulgaris

text: XhoI

text: PstI

text: Hydra EST -IV

text: plasmid

text: pBS SK-


text: National Institute of Genetics

text: live

text: Generic

text: synonym: Hydra magnipapillata

text: adult budding stage

text: Hydra vulgaris

text: whole body

text: Hydra magnipapillata cDNA library


text: live

text: Generic

text: pGEM-T Vector( from Promega) is ampicillin resistent, SP6 and T7 primers should be used for sequencing. Non-directionaly cloned

text: Hydra vulgaris

text: EcoRI

text: EcoRI

text: Hydra EST -Kiel 2

text: plasmid

text: pGEM-T


text: live

text: Generic

text: TransforMax EC100 (Epicentre), T1 Phage resistant electrocompetent cells

text: Hydra vulgaris

text: NotI

text: EcoRI

text: sf-1 mutant of Hydra magnipapillata

text: Hydra EST Darmstadt I

text: plasmid

text: pBluescript II SK (+)


text: live

text: Generic

text: (pGEM-T Vector( from Promega) is ampicillin resistent, M13 forward and M13 reverse, or SP6 primers should be used for sequencing. Because of the construction peculiarities T7 primer can not be used for sequencing.

text: Hydra vulgaris

text: EcoRI

text: EcoRI

text: Hydra EST -Kiel 5

text: plasmid

text: pGEM-T


text: live

text: Generic

text: a.1st strand cDNA was primed with a Not I primer-adapter (5' - pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3') b.Double-stranded cDNA was ligated to Sal I adapter, digested with Not I and cloned into the pSPORT 1-vector pre-cut with Not I and Sal I. c.The ligation mix was transformed into DH10B cells. d.The cells were grown in SOC = 5g yeast, 20g tryptone, 0.5 g NaCl, 10 mM MgSO4, 10 mM MgCl, 0.2% glucose/Liter, (no antibiotic). e.DMSO was added to a final conc. of 10% as a cryoprotectant.and frozen f.The titre before freezing was determined as ~2400/100 ul. Assuming a 10% loss upon freezing, the titre is probably ~2100/ 100 ul. g.9 tubes each containing ~ 2100 clones/100 ul [ = total of ~19,000] are enclosed. h.The frequency of vectors containing inserts is 96% as determined by digestion check after picking 24 clones, miniprep and subsequent digestion with Not I and Sal I. i.A low level of 32P was used in the cDNA synthesis procedure. The level measured by holding a Geiger Counter next to a tube was background.

text: Hydra vulgaris

text: Not I

text: Sal I

text: Hydra EST UCI 5

text: plasmid


text: live

text: Generic

text: a.1st strand cDNA was primed with a Not I primer-adapter (5' -pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3') b.Double-stranded cDNA was ligated to Sal I adapter, digested with Not I and cloned into the pSPORT 1-vector pre-cut with Not I and Sal I. c.The ligation mix was transformed into DH10B cells. d.The picked clones were grown up in the 384-plate containing the freezing medium (LB-Medium containing 4 % glycerol w/v, and different salts) (antibiotic is carbenicillin 100ug/ml). e.The frequency of vectors containing inserts is 96% as determined by digestion check after picking 20 clones, miniprep and subsequent digestion with Not I and Sal I. f.A low level of 32P was used in the cDNA synthesis procedure. The level measured by holding a Geiger Counter next to a plate was background.

text: ElectroMAX DH10B cells, Invitrogen

text: Hydra vulgaris

text: Not I

text: Sal I

text: Hydra EST UCI 5 ALP

text: plasmid


text: live

text: Generic

text: a. 1st strand cDNA was primed with a Not I primer-adapter(5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' b. Double-stranded cDNA was ligated to Sal I adapter, digested with NotI and cloned into the pSPORT1 vector pre-cut with NotI and SalI. c. The ligation mix was transformed into DH10B cells. d. The cells were grown in SOC=5g yeast, 20g tryptone, 0.5g NaCl, 10mM MgSO4, 10mM MgCl, 0.2% glucose/liter, (no antibiotic). e. DMSO was added to a final conc. of 10% as a cryoprotectant and frozen. f. The titre before freezing was determined as 15000/100ul. Assuming a 10% loss upon freezing, the titre is probably ~ 13500clones/ul. g. 3 tubes each containing ~ 13500 clones/ul [=total of ~40,500] are enclosed. h. The frequency of vectors containing inserts is 90% as determined by digestion check after picking 20 clone, miniprep and subsequent digestions with NotI and Sal I. i. A low level of 32P was used in the cDNA synthesis procedure. The level measured by holding a Geiger Counter next to a tube was background.

text: Hydra vulgaris

text: Not I

text: Sal I

text: Hydra EST UCI 6

text: plasmid


text: live

text: Generic

text: (pGEM-T Vector( from Promega) is ampicillin resistent, M13 forward and M13 reverse, or SP6 and T7 primers should be used for sequencing.)

text: Hydra vulgaris

text: Hydra EST Kiel 7

text: plasmid

text: pGEM-T


text: live

text: Generic

text: a. 1st strand cDNA was primed with a Not I primer-adapter(5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' b. Double-stranded cDNA was ligated to Sal I adapter, digested with NotI and cloned into the pSPORT1 vector pre-cut with NotI and SalI. c. The ligation mix was transformed into DH10B cells. d. The cells were grown in SOC=5g yeast, 20g tryptone, 0.5g NaCl, 10mM MgSO4, 10mM MgCl, 0.2% glucose/liter, (no antibiotic) e. DMSO was added to a final conc. of 10% as a cryoprotectant and frozen. f. The titre before freezing was determined as 15000/100ul. Assuming a 10% loss upon freezing, the titre is probably ~ 13500clones/ul g. 3 tubes each containing ~ 13500 clones/ul [=total of ~40,500] are enclosed. h. The frequency of vectors containing inserts is 90% as determined by digestion check after picking 20 clone, miniprep and subsequent digestions with NotI and Sal I. i. A low level of 32P was used in the cDNA synthesis procedure. The level measured by holding a Geiger Counter next to a tube was background.

text: Hydra vulgaris

text: Not I

text: Sal I

text: Hydra UCI6- barcoded EST's

text: plasmid


text: live

text: Generic

text: a. 1st strand cDNA was primed with a Not I primer-adapter (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3') b. Double-stranded cDNA was ligated to Sal I adapter, digested with Not I and cloned into the pSPORT 1-vector pre-cut with Not I and Sal I. c. The ligation mix was transformed into DH10B cells. d. The cells were grown in SOC = 5g yeast, 20g tryptone, 0.5 g NaCl, 10 mM MgSO4, 10 mM MgCl, 0.2% glucose / Liter, (no antibiotic). e. DMSO was added to a final conc. of 10% as a cryoprotectant.and frozen. f. The titre before freezing was determined as ~ 6000/ 100 ul. Assuming a 10% loss upon freezing, the titre is probably ~ 5400/ 100 ul. g. 6 tubes each containing ~ 5400 clones/ul [ = total of ~ 32,400] are enclosed. h. The frequency of vectors containing inserts is 95% as determined by digestion check after picking 20 clones, miniprep and subsequent digestion with Not I and Sal I. i. A low level of 32P was used in the cDNA synthesis procedure. The level measured by holding a Geiger Counter next to a tube was background.

text: Hydra vulgaris

text: Not I

text: Sal I

text: Hydra EST UCI 7

text: plasmid


text: live

text: Generic

text: a. 1st strand cDNA was primed with a Not I primer-adapter (5' ? pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3') b. Double-stranded cDNA was ligated to Sal I adapter, digested with Not I and cloned into the pSPORT 1-vector pre-cut with Not I and Sal I. c. The ligation mix was transformed into DH10B cells. d. The cells were grown in SOC = 5g yeast, 20g tryptone, 0.5 g NaCl, 10 mM MgSO4, 10 mM MgCl, 0.2% glucose / Liter, (no antibiotic). e. DMSO was added to a final conc. of 10% as a cryoprotectant.and frozen f. The titre before freezing was determined as ~6000/ 100 ul. Assuming a 10% loss upon freezing, the titre is probably ~5400/100 ul. g. 3 tubes each containing ~ 5400 clones/ul [ = total of ~ 16,200] are enclosed. h. The frequency of vectors containing inserts is 95% as determined by digestion check after picking 20 clones, miniprep and subsequent digestion with Not I and Sal I. i. A low level of 32P was used in the cDNA synthesis procedure. The level measured by holding a Geiger Counter next to a tube was background.

text: adult animals

text: Hydra vulgaris

text: Not I

text: Sal I

text: whole animal, alsterpaullone treated

text: plasmid


text: live

text: Generic

text: a.1st strand cDNA was primed with a Not I primer-adapter (5' - pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3') b.Double-stranded cDNA was ligated to Sal I adapter, digested with Not I and cloned into the pSPORT 1-vector pre-cut with Not I and Sal I. c.The ligation mix was transformed into DH10B cells. d.The cells were grown in SOC = 5g yeast, 20g tryptone, 0.5 g NaCl, 10 mM MgSO4, 10 mM MgCl, 0.2% glucose / Liter, (no antibiotic). e.DMSO was added to a final conc. of 10% as a cryoprotectant and frozen. f.The titre before freezing was determined as ~9000/ 100 ul. Assuming a 10% loss upon freezing, the titre is probably ~ 8000/ 100 ul. g.5 tubes each containing ~ 8000 clones/ul [ = total of ~ 40,000] are enclosed. h.The frequency of vectors containing inserts is 90% as determined by digestion check after picking 20 clones, miniprep and subsequent digestion with Not I and Sal I.

text: Adult animals

text: Electro Max DH10B cells

text: Hydra vulgaris

text: Not I

text: Sal I

text: male

characteristic: sex

text: plasmid


characteristic: INSDC center name

text: live

text: Generic

text: a.1st strand cDNA was primed with a Not I primer-adapter (5' - pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3') b.Double-stranded cDNA was ligated to Sal I adapter, digested with Not I and cloned into the pSPORT 1-vector pre-cut with Not I and Sal I. c.The ligation mix was transformed into DH10B cells. d.The cells were grown in SOC = 5g yeast, 20g tryptone, 0.5 g NaCl, 10 mM MgSO4, 10 mM MgCl, 0.2% glucose / Liter, (no antibiotic). e.DMSO was added to a final conc. of 10% as a cryoprotectant and frozen. f.The titre before freezing was determined as ~10000/ 100 ul. Assuming a 10% loss upon freezing, the titre is probably ~ 9000/ 100 ul. g.5 tubes each containing ~ 9000 clones/ul [ = total of ~ 45,000] are enclosed. h.The frequency of vectors containing inserts is 90% as determined by digestion check after picking 20 clones, miniprep and subsequent digestion with Not I and Sal I. i.A low level of 32P was used in the cDNA synthesis procedure. The level measured by holding a Geiger Counter next to a tube was background.

text: Adult animals

text: Electro Max DH10B cells

text: Hydra vulgaris

text: Not I

text: Sal I

text: female

text: plasmid


text: Wuhan University

characteristic: INSDC first public

text: live

text: Generic

text: Adult

text: Scorpiops margerisonae

text: Venom gland

text: Scorpiops margerisonae venom gland Library


text: Genoscope - Centre National de Sequencage

characteristic: INSDC last update

text: live

text: Generic

text: Total RNA was extracted from shock-frozen tissue; mRNA was purified on Ambion's poly(A)Purist columns. cDNAS was synthesised by the oligo-capping method described by Maruyama, K. and Sugano, S. (1994) Gene 138: 171-174 and by Suzuki, Y. et al. (1997) Gene 200: 149-156 in order to enrich full-length cDNAs. The molecules were the inserted into the pDONR222.1 (Invitrogen), cloned and sequenced at the Genoscope, Evry, France by Applied Biosystems 3730 automated sequencers.

text: Rattus norvegicus

text: Sprague-Dawley

text: spleen

text: Rattus norvegicus spleen Sprague-Dawley


text: live

text: Generic

text: Rattus norvegicus

text: Sprague-Dawley

text: 11-12 days foetus

text: Rattus norvegicus 11-12 days foetus Sprague-Dawley

characteristic: vector


text: Institute of Oceanology, Chinese Academy of Sciences

text: live

characteristic: INSDC status

text: Generic

text: Apostichopus japonicus

text: body wall

text: sea cucumber heat shock suppression subtractive hybridization library


text: Rural Development Administration

text: live

text: Generic

characteristic: NCBI submission model

text: Accession PI 534875

text: 5-8 days after flowering

text: E.coli DH5a

text: Cuphea paucipetala

text: EcoRI

text: embryo

text: Cuphea paucipetala PI 534875 embryo

text: plasmid

text: pDNR-LIB


text: Institute of Genetic and Developmental Biology, Chinese Academy of Sciences

text: live

text: Generic

characteristic: NCBI submission package

text: Salt-responsive genes of Atriplex centralasiatica seedlings through suppression subtractive hybridization (SSH)

text: Atriplex centralasiatica

text: seedlings

text: Atriplex centralasiatica seedlings, salt-induced SSH


text: Nanjing University of Chinese Medicine

text: live

text: Generic

text: flower buds of early-bolting Angelica sinensis (Oliv.) Diels with sizes of about 0.5 cm

text: Angelica sinensis

text: hermaphrodite

text: flower buds

text: Angelica sinensis Flower Buds


text: Institute of Zoology, University of Innsbruck

text: live

text: Generic

characteristic: cell type

text: Differential Display PCR on cDNA extracted from Rainbow Trout gill pavement cells in single seeded insert cultures after 24h apical freshwater (asymetrical) or culture medium (symetrical)

text: adult

text: gill

characteristic: organ

text: Oncorhynchus mykiss

text: epithelium

text: ddPCR of Rainbow Trout gill pavement cells


text: USGS

text: live

text: Generic

text: Differentially expressed genes in L. atricilla liver that was exposed to MeHg at day 3 of incubation

characteristic: description

text: Hatchling

text: Leucophaeus atricilla

characteristic: organism

text: Liver

text: Laughing gull liver differentially expressed genes


text: Kunming Institute of Zoology, Chinese Academy of Sciences (CAS)

text: live

text: Generic

text: venom glands

text: Bungarus multicinctus

text: venom glands

text: Kraits snake Bungarus multicinctus cDNA library

characteristic: title


text: live

text: Generic

text: venom glands

text: Naja atra

text: venom glands

text: Cobras snake Naja atra cDNA library


text: ITQB-UNL Instituto Tecnologia Quimica e Biologica, Universidade Nova de Lisboa

text: live

text: Generic

characteristic: cultivar

text: 14 days germinated seedlings

characteristic: dev-stage

text: Shoot

text: Oryza sativa Indica Group

text: Oryza sativa (indica cultivar-group) cv. PSBRc1 cDNA-AFLP fragment


text: IARI

text: live

text: Generic

text: Sequences are obtained from juvenile second stage Anguina tritici, commonly known as seed gall nematode, by subtractive hybridization.

text: Anguina tritici

text: GTAAAACGACGGCCAGTG

text: GGAAACAGCTATGACCATG

text: Whole worm

characteristic: tissue-type

text: Anguina tritici cDNA sequences J2 stage


characteristic: External Id

text: live

text: Generic

text: seedling

text: Zea mays

text: seedling

text: Zea mays cadmium induced library


text: Instituto Potosino de Investigacion Cientifica y Tecnologica

text: live

text: Generic

text: Cactus pear. The EST sequence was obtained from a cDNA library of twelve-month-old cladodes of Opuntia streptacantha plants subjected to several abiotic stresses (drought, salinity, heat and cold), and the exogenous application of ABA. The library was generated using the Creator Smart cDNA library construction kit as recommended by manufacture's (Clontech)

text: seedling

text: E.coli

characteristic: lab-host

text: Opuntia streptacantha

text: sfilA

text: sfilB

text: cladode

text: Opuntia cDNA library under abiotic stress

text: plasmid

text: pDNR-LIB


text: University of Liege, Gembloux Agro-Bio Tech

characteristic: INSDC center name

text: live

text: Generic

text: Grande-Naine

text: Sequence obtained by cDNA-AFLP.

text: Musa acuminata AAA Group

text: Fruit crown tissues


text: The University of Tokyo, Laboratory of Insect Genetics and Bioscience

characteristic: INSDC first public

text: live

text: Generic

text: BmN cells

characteristic: cell-line

text: BmN cell line was established from the ovary, female

text: Bombyx mori

text: cDNA library from BmNPV-infected BmN cells


text: Embrapa Recursos Geneticos e Biotecnologia

characteristic: INSDC last update

text: live

text: Generic

text: Coffea eugenioides


text: University of Ljubljana, Biotechnical faculty, Agronomy Department

text: live

characteristic: INSDC status

text: Generic

text: Wye Target, Celeia

text: Differentially expressed cDNAs obtained with cDNA-AFLP technique were cloned into plasmid vector

text: Humulus lupulus

text: female

characteristic: sex

text: Hop cDNA-AFLP library

text: Plasmid

text: pBlueScript II

characteristic: vector


text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Wye Target, Celeia

text: Differentially expressed cDNAs obtained with Genesnare techniques were cloned into plasmid vector

text: Humulus lupulus

text: female

text: Hop GeneSnare library

text: Plasmid

text: pBlueScript II


text: National Cheng-Kung University, Taiwan

text: live

text: Generic

text: Suppression subtractive hybridization-based strategy

characteristic: description

text: 24 month old

text: Phalaenopsis amabilis

text: Taiwan sugar TS97K P. amabilis W1-10 x P. amabilis W1-22

characteristic: strain

text: Leaves

text: Library for Phalaenopsis amabilis orchid infected with Erwinia chrysanthemi (PE)


text: College of Life Science, Henan Agricultural University

text: live

text: Generic

text: root

characteristic: organ

text: Nicotiana tabacum

characteristic: organism

text: root

text: Nicotiana tabacum supression subtractive hybridization library

characteristic: title


text: Sichuan Agricultural University

text: live

text: Generic

text: Induced by wheat stripe rust (Puccinia striiformis f. sp. tritici).

text: Triticum aestivum

text: SSH-cDNA library induced by wheat stripe rust (Puccinia striiformis f. sp. tritici)


text: Institute of Life and Health Engineering

text: live

text: Generic

text: larvae

characteristic: dev-stage

text: E.coli DH5a

text: Baliga sagax

text: Sfi I

text: Sfi I

text: Whole larvae

characteristic: tissue-type

text: Myrmeleon sagax cDNA library

text: Plasmid

text: pDNR-LIB


characteristic: External Id

text: Boyce Thompson Institute for Plant Research

text: live

text: Generic

text: Intrigue

characteristic: cultivar

text: Root

text: Festuca rubra subsp. commutata

text: Sfi 1A

text: Sfi 1B

text: Root of Chewings fescue cultivar 'Intrigue'

text: pDNR-LIB


text: NRC on Plant Biotechnology, IARI

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: ICC4958 and ICC1882

text: Chickpea drought tolerant cultivar ICC4958 and susceptible cultivar ICCC1882 was used in this study. Plants were grown in pots under near normal optimal condition in glasshouse. Control plants (well watered) were maintained close to 80% field capacity by keeping the pot wet to that level every day by compensating for the loss due to transpiration and water stressed plants were exposed to gradual water stress by partly compensated for the water loss due to transpiration. Plant samples from the control (well watered) and stressed plants were harvested when the transpiration ratio reached to 0.1 in the water stressed plants. Total RNA from root tissue was isolated using Trizol reagent and mRNA was extracted from the total RNA using poly(A) tract mRNA Purification Kit (Promega). Forward SSH library was constructed using subtraction of cDNA synthesized from water stressed ICC4958 plants as tester and that from the water stressed ICC1882 plants as driver with the Clontech PCR-Select cDNA subtraction Kit (CLONTECH, USA) according to the manufacturer's protocol. Differentially expressed cDNAs were cloned in pGEMT-Easy vector and transformed into E.Coli. Sanger sequencing of the selected clones were done using universal sequencing primers T7, SP6 and M13.

text: Flowering stage

text: Cicer arietinum

text: Root

text: Chickpea drought stressed cDNA SSH library AB1

text: pGEM-T Easy vector


text: NRC on Plant Biotechnology, IARI

characteristic: INSDC last update

text: live

text: Generic

text: ICC4958 and ICC1882

text: Chickpea drought tolerant cultivar ICC4958 and susceptible cultivar ICCC1882 was used in this study. Plants were grown in pots under near normal optimal condition in glasshouse. Control plants (well watered) were maintained close to 80% field capacity by keeping the pot wet to that level every day by compensating for the loss due to transpiration and water stressed plants were exposed to gradual water stress by partly compensated for the water loss due to transpiration. Plant samples from the control (well watered) and stressed plants were harvested when the transpiration ratio reached to 0.1 in the water stressed plants. Total RNA from root tissue was isolated using Trizol reagent and mRNA was extracted from the total RNA using poly(A) tract mRNA Purification Kit (Promega). Reverse SSH library was constructed using subtraction of cDNA synthesized from water stressed ICC1882 plants as tester and that from the water stressed ICC4958 plants as driver with the Clontech PCR-Select cDNA subtraction Kit (CLONTECH, USA) according to the manufacturer's protocol. Differentially expressed cDNAs were cloned in pGEMT-Easy vector and transformed into E.Coli. Sanger sequencing of the selected clones were done using universal sequencing primers T7, SP6 and M13.

text: Flowering stage

text: Cicer arietinum

text: Root

text: Chickpea drought stressed cDNA SSH library AB2

text: pGEM-T Easy vector


text: NRC on Plant Biotechnology, IARI

text: live

characteristic: INSDC status

text: Generic

text: Chickpea drought tolerant cultivar ICC4958 and susceptible cultivar ICCC1882 was used in this study. Plants were grown in pots under near normal optimal condition in glasshouse. Control plants (well watered) were maintained close to 80% field capacity by keeping the pot wet to that level every day by compensating for the loss due to transpiration and water stressed plants were exposed to gradual water stress by partly compensated for the water loss due to transpiration. Plant samples from the control (well watered) and stressed plants were harvested when the transpiration ratio reached to 0.1 in the water stressed plants. Total RNA from root tissue was isolated using Trizol reagent and mRNA was extracted from the total RNA using poly(A) tract mRNA Purification Kit (Promega). Forward SSH library was constructed using subtraction of cDNA synthesized from water stress plants as tester and that from the control plants as driver with the Clontech PCR-Select cDNA subtraction Kit (CLONTECH, USA) according to the manufacturer's protocol. Differentially expressed cDNAs were cloned in pGEMT-Easy vector and transformed into E.Coli. Sanger sequencing of the selected clones were done using universal sequencing primers T7, SP6 and M13.

text: Flowering stage

text: Cicer arietinum

text: Root

text: Chickpea drought stressed cDNA SSH library AR1

text: pGEM-T Easy vector


text: NRC on Plant Biotechnology, IARI

text: live

text: Generic

text: Chickpea drought tolerant cultivar ICC4958 and susceptible cultivar ICCC1882 was used in this study. Plants were grown in pots under near normal optimal condition in glasshouse. Control plants (well watered) were maintained close to 80% field capacity by keeping the pot wet to that level every day by compensating for the loss due to transpiration and water stressed plants were exposed to gradual water stress by partly compensated for the water loss due to transpiration. Plant samples from the control (well watered) and stressed plants were harvested when the transpiration ratio reached to 0.1 in the water stressed plants. Total RNA from root tissue was isolated using Trizol reagent and mRNA was extracted from the total RNA using poly(A) tract mRNA Purification Kit (Promega). Reverse SSH library was constructed using subtraction of cDNA synthesized from control plants as tester and that from the water stressed plants as driver with the Clontech PCR-Select cDNA subtraction Kit (CLONTECH, USA) according to the manufacturer's protocol. Differentially expressed cDNAs were cloned in pGEMT-Easy vector and transformed into E.Coli. Sanger sequencing of the selected clones were done using universal sequencing primers T7, SP6 and M13.

text: Flowering stage

text: Cicer arietinum

text: Root

text: Chickpea drought stressed cDNA SSH library AR2

text: pGEM-T Easy vector


text: NRC on Plant Biotechnology, IARI

text: live

text: Generic

text: Chickpea drought tolerant cultivar ICC4958 and susceptible cultivar ICCC1882 was used in this study. Plants were grown in pots under near normal optimal condition in glasshouse. Control plants (well watered) were maintained close to 80% field capacity by keeping the pot wet to that level every day by compensating for the loss due to transpiration and water stressed plants were exposed to gradual water stress by partly compensated for the water loss due to transpiration. Plant samples from the control (well watered) and stressed plants were harvested when the transpiration ratio reached to 0.1 in the water stressed plants. Total RNA from leaf tissue was isolated using Trizol reagent and mRNA was extracted from the total RNA using poly(A) tract mRNA Purification Kit (Promega). Forward SSH library was constructed using subtraction of cDNA synthesized from water stress plants as tester and that from the control plants as driver with the Clontech PCR-Select cDNA subtraction Kit (CLONTECH, USA) according to the manufacturer's protocol. Differentially expressed cDNAs were cloned in pGEMT-Easy vector and transformed into E.Coli. Sanger sequencing of the selected clones were done using universal sequencing primers T7, SP6 and M13.

text: Flowering stage

text: Cicer arietinum

text: Shoot

text: Chickpea drought stressed cDNA SSH library AS1

text: pGEM-T Easy vector


text: NRC on Plant Biotechnology, IARI

text: live

text: Generic

text: Chickpea drought tolerant cultivar ICC4958 and susceptible cultivar ICCC1882 was used in this study. Plants were grown in pots under near normal optimal condition in glasshouse. Control plants (well watered) were maintained close to 80% field capacity by keeping the pot wet to that level every day by compensating for the loss due to transpiration and water stressed plants were exposed to gradual water stress by partly compensated for the water loss due to transpiration. Plant samples from the control (well watered) and stressed plants were harvested when the transpiration ratio reached to 0.1 in the water stressed plants. Total RNA from leaf tissue was isolated using Trizol reagent and mRNA was extracted from the total RNA using poly(A) tract mRNA Purification Kit (Promega). Reverse SSH library was constructed using subtraction of cDNA synthesized from control plants as tester and that from the water stressed plants as driver with the Clontech PCR-Select cDNA subtraction Kit (CLONTECH, USA) according to the manufacturer's protocol. Differentially expressed cDNAs were cloned in pGEMT-Easy vector and transformed into E.Coli. Sanger sequencing of the selected clones were done using universal sequencing primers T7, SP6 and M13.

text: Flowering stage

text: Cicer arietinum

text: Shoot

text: Chickpea drought stressed cDNA SSH library AS2

text: pGEM-T Easy vector


text: NRC on Plant Biotechnology, IARI

text: live

text: Generic

text: RILs derived from cross between ICC4958 and ICC1882

text: A RIL population was derived from cross between ICC4958 a high root biomass genotype (HRB) and ICC1882 a low root biomass(LRB) genotype. 10 extreme RILs exhibiting HRB and LRB were selected. Bulks of the 10 RILs were used for construction of cDNA SSH library. RILs were grown in pots under near normal optimal condition in glasshouse. Control plants (well watered) were maintained close to 80% field capacity by keeping the pot wet to that level every day by compensating for the loss due to transpiration and water stressed plants were exposed to gradual water stress by partly compensated for the water loss due to transpiration. Plant samples from the control (well watered) and stressed plants were harvested when the transpiration ratio reached to 0.1 in the water stressed plants. Total RNA from root tissue was isolated using Trizol reagent and equal amount of total RNA from 10 HRB RIL and 10 LRB were mixed to produce two Bulks of RNA, further mRNA was extracted from the total RNA using poly(A) tract mRNA Purification Kit (Promega). Forward SSH library was constructed using subtraction of cDNA synthesized from water stressed bulk of 10 HRB RILs plants as tester and that from the bulk of 10 RILs with LRB plants as driver with the Clontech PCR-Select cDNA subtraction Kit (CLONTECH, USA) according to the manufacturer's protocol. Differentially expressed cDNAs were cloned in pGEMT-Easy vector and transformed into E.Coli. Sanger sequencing of the selected clones were done using universal sequencing primers T7, SP6 and M13.

text: Flowering stage

text: Cicer arietinum

text: Root

text: Chickpea drought stressed cDNA SSH library BULK1

text: pGEM-T Easy vector


text: NRC on Plant Biotechnology, IARI

text: live

text: Generic

text: A RIL population was derived from cross between ICC4958 a high root biomass genotype and ICC1882 a low root biomass genotype. 10 extreme RILs exhibiting HRB and LRB were selected. Bulks of the 10 RILs were used for construction of cDNA SSH library. RILs were grown in pots under near normal optimal condition in glasshouse. Control plants (well watered) were maintained close to 80% field capacity by keeping the pot wet to that level every day by compensating for the loss due to transpiration and water stressed plants were exposed to gradual water stress by partly compensated for the water loss due to transpiration. Plant samples from the control (well watered) and stressed plants were harvested when the transpiration ratio reached to 0.1 in the water stressed plants. Total RNA from root tissue was isolated using Trizol reagent and equal amount of total RNA from 10 HRB RIL and 10 LRB were mixed to produce two Bulks of RNA, further mRNA was extracted from the total RNA using poly(A) tract mRNA Purification Kit (Promega). Reverse SSH library was constructed using subtraction of cDNA synthesized from water stressed bulk of 10 LRB RILs plants as tester and that from the bulk of 10 RILs with HRB plants as driver with the Clontech PCR-Select cDNA subtraction Kit (CLONTECH, USA) according to the manufacturer's protocol. Differentially expressed cDNAs were cloned in pGEMT-Easy vector and transformed into E.Coli. Sanger sequencing of the selected clones were done using universal sequencing primers T7, SP6 and M13.

text: Flowering stage

text: Cicer arietinum

text: Root

text: Chickpea drought stressed cDNA SSH library BULK2

text: pGEM-T Easy vector


text: Warsaw University of Life Sciences - SGGW

text: live

text: Generic

text: Fidelio

text: kernel

text: x Triticosecale

text: triticale cDNA fragments


text: live

text: Generic

text: Fidelio

text: leaves

text: x Triticosecale

text: triticale leaves cDNA fragments


text: Johannes Gutenberg University of Mainz

text: live

text: Generic

text: Echinorhynchus truttae

text: Echinorhynchus truttae library (Hankeln T)


text: Johannes Gutenberg University of Mainz

text: live

text: Generic

text: Pomphorhynchus laevis

text: Pomphorhynchus laevis library (Hankeln T)


text: Johannes Gutenberg University of Mainz

text: live

text: Generic

text: Brachionus plicatilis

text: Brachionus plicatilis library (Hankeln T)


text: MPI for Molecular Genetics

text: live

text: Generic

text: country: Switzerland ; lat_lon: 47.46 N 7.57 E ; collection_date: Jun-2007 ; collected_by: Sadd B.M.; Lab-raised worker offspring of field caught spring queens were used. Total RNA was extracted from 2 female worker offspring from each of 4 colonies. cDNA was synthesized using the SMART approach (Mint-Universal cDNA synthesis kit, Evrogen, Russia), subsequently normalized using duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). Plasmids from the normalized libraries were 5' end sequenced using the pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads from 3730XL capillary sequencers (ABI) were base-called using Phred (http://www.phrap.org). Library construction and sequencing were performed at the MPI for Molecular Genetics Berlin.

text: adult

text: Bombus terrestris

text: female

characteristic: sex

text: Thorax and abdomen


text: Genoscope - Centre National de Sequencage

text: live

text: Generic

text: Tuber melanosporum

text: fruit body

text: Tuber melanosporum fruit body


text: live

text: Generic

text: Tuber melanosporum

text: mycelium

text: Tuber melanosporum mycelium


text: live

text: Generic

text: Pea aphid whole body full-insert cDNA library constructed by Nathalie Leterme (INRA Rennes, France)

text: Acyrthosiphon pisum

text: oviparous sexual females

text: whole insect

text: Acyrthosiphon pisum whole insect oviparous sexual females


text: live

text: Generic

text: Acyrthosiphon pisum

text: males

text: whole insect

text: Acyrthosiphon pisum whole insect males


text: Universita' degli Studi di Padova

text: live

text: Generic

text: The library was made from dT primed cDNA and cloned into the pDNR-LIB vector (Clontech; Takara Bio Inc, Japan). PolyA RNA was primed with a CDS III/3' PCR Primer (5'-ATTCTAGAGGCCGAGGCGGCCGACATG-d(T)30VN-3'; N=A,G,C,T; V=A,G,C). The second strand was synthesized and an SMART IV Oligonucleotide was ligated to the 5' end (5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3'). cDNA was inserted into the vector using site specific recombination (flanking SfiI sites on cDNA). Library was constructed and sequencing was performed at the laboratory of Dr. Richard Reinhardt at the Max Planck Institute for Molecular Genetics, Berlin (Germany) during the NoE (Marine Genomics Europe) training course 'Generation of cDNA Libraries by Primer Extension' (20 November - 1 December, 2006).

text: zooids at different blastogenic stages

text: Botryllus schlosseri

text: SfiIA

text: SfiIB

text: hermaphrodite

text: whole animal

text: B. schlosseri directionally cloned enriched full-length cDNA library, 5' end sequencing

text: Plasmid

text: pDNR-LIB

characteristic: vector


text: live

text: Generic

characteristic: NCBI submission model

text: Hortenso

text: kernel

text: x Triticosecale

text: triticale kernel cDNA fragments


text: live

text: Generic

characteristic: NCBI submission package

text: Fidelio

text: kernel

text: x Triticosecale

text: triticale Fidelio kernel cDNA fragments


text: Korea Research Institute of Bioscience and Biotechnology

text: live

text: Generic

text: Phanerodontia chrysosporium

text: KFRI 20741

text: whole body

text: Phanerochaete chrysosporium KFRI 20741 whole body


text: Fisheries and Oceans Canada

text: live

text: Generic

text: Tissue contributors: R.J. Nelson, K. Young (Institute for Ocean Sciences, Sidney BC)

characteristic: description

text: whole

text: Boreogadus saida

text: whole

text: bsaievw whole Boreogadus saida cDNA


text: Universiti Kebangsaan Malaysia

text: live

text: Generic

text: This research focuses mainly on the construction cDNA-AFLP transcripts profiling of salicylic acid-elicited Polygonum minus. By cDNA-AFLP analysis, the expression profiles of 2 mM salicylic acid elicited Polygonum minus was compared with the control plants. Total RNA from treated and control Polygonum minus leaves were extracted using the modified Lopez-Gomez and Gomez Lim, 1992 method. The total RNA was then subjected to mRNA purification using PolyATtract mRNA Isolation Kit. cDNAs were synthesized and AFLP was performed by using the AFLP Expression Analysis Kit (Li-Cor Biosciences, USA) with 64 different primer combinations. From the cDNA-AFLP based transcript profiling obtained, trasncript-derived fragments that showed up-regulated patterns in the treated plants as compared to the controlled plants were identified, excised from the gel, reamplified and cloned into pGEM-T Easy Vectors before transforming into E. coli Top 10 cells. Plasmids were extracted, checked for inserts through colony PCR before sending for sequencing.

text: E. coli strain TOP 10

characteristic: lab-host

text: Leaves

text: Persicaria minor

text: Salicylic acid-treated x non-treated Polygonum minus leaves cDNA-AFLP profiles library

text: pGEM-T Easy Vector


text: Anhui Agricultural University

text: live

text: Generic

text: Longjing 43

text: E.coli DH5a

text: Camellia sinensis

text: Root

text: roots of tea plant induced by Ectropic oblique feeding cDNA-AFLP


text: Anhui Agricultural University

text: live

text: Generic

text: Shuchazao

text: E.coli DH5a

text: Camellia sinensis

text: Leaf

text: leaves of tea plant induced by Empoasca vitis Gothe feeding


text: University of Lausanne

text: live

text: Generic

text: Bone marrow-derived macrophages

characteristic: cell type

text: Mus musculus

characteristic: strain

text: Murine bone marrow-derived macrophages (T)

text: Plasmid

text: pCR-Blunt


text: North Carolina State University

text: live

text: Generic

text: adult

text: Ceraphronidae sp. BJS-2010

characteristic: organism

text: SfiIA

text: SfiIB

text: female

text: whole organism

text: Ceraphronidae sp. whole body female library

characteristic: title

text: Phage

text: Lambda TriplEx2


text: North Carolina State University

text: live

text: Generic

text: adult

characteristic: dev-stage

text: Campoletis sonorensis

text: SfiIA

text: SfiIB

text: whole organism

characteristic: tissue-type

text: Campoletis sonorensis whole body mixed males and females library

text: Phage

text: Lambda TriplEx2


characteristic: External Id

text: North Carolina State University

text: live

text: Generic

text: adult

text: Eucoilinae sp. BJS-2010

text: SfiIA

text: SfiIB

text: female

text: whole organism

text: Figitidae Eucoilinae sp. whole body females library

text: Phage

text: Lambda TriplEx2


text: North Carolina State University

characteristic: INSDC center name

text: live

text: Generic

text: adult

text: Neodiprion sertifer

text: SfiIA

text: SfiIB

text: whole organism

text: Neodiprion sertifer whole body mixed males and females library

text: Phage

text: Lambda TriplEx2


text: North Carolina State University

characteristic: INSDC first public

text: live

text: Generic

text: adult

text: Pelecinus polyturator

text: SfiIA

text: SfiIB

text: female

text: whole organism

text: Pelecinus polyturator whole body females library

text: Phage

text: Lambda TriplEx2


text: North Carolina State University

characteristic: INSDC last update

text: live

text: Generic

text: adult

text: Pristaulacus strangaliae

text: SfiIA

text: SfiIB

text: female

text: whole organism

text: Pristaulacus strangaliae whole body females library

text: Phage

text: Lambda TriplEx2


text: Helmholtz Zentrum munich

text: live

characteristic: INSDC status

text: Generic

text: Fagus sylvatica

text: leave


text: International Centre for Genetic Engineering and Biotechnology and Directorate of Rice Research

text: live

text: Generic

text: Suraksha

characteristic: cultivar

text: Shoot

characteristic: organ

text: Oryza sativa Indica Group

text: Subtracted cDNA library from Indica rice variety Suraksha

text: Phage


text: live

text: Generic

characteristic: NCBI submission model

text: Sanger metagenomic sequences generated by vector-enabled metagenomics (VEM) from purified viruses collected from whiteflies in Citra, Florida.

text: uncultured virus

text: WhitefliesVemCitraFl

text: TOPO pCR 4

characteristic: vector


text: live

text: Generic

characteristic: NCBI submission package

text: Sanger metagenomic sequences generated by vector-enabled metagenomics (VEM) from purified viruses collected from whiteflies in Homestead, Florida.

characteristic: description

text: uncultured virus

text: WhitefliesVemHomesteadFl

text: TOPO pCR 4


text: James Cook University

text: live

text: Generic

text: Acropora millepora

text: Hermaphrodite

characteristic: sex

text: Acropora millepora mixed stages library


text: Cotton Research Institute, Chinese Academy of Agricultural Sciences

text: live

text: Generic

text: 3-10 weeks after planting

text: Gossypium hirsutum

text: cotton shoot apical meristems, buds and flowers

text: Plasmid

text: pDNR-LIB


text: INRA - UMR BIOGECO

text: live

text: Generic

text: country: France:Ciron Valley. The cDNA library was made using mRNA extracted from setting buds harvested on 3 adult European beech located in the Ciron Valley (France). The library was built using the Clone Miner cDNA library construction kit (Invitrogen) according to the manufacturer's instructions

text: quiescent buds

text: Fagus sylvatica

text: bud

text: WZOAFSCA


text: Vasantdada Sugar Institute

text: live

text: Generic

text: Co 62175

text: messenger RNA was extracted from control and salt stress induced sugarcane leaf tissue at 15 and 30 days after planting and transcript-derived fragments were obtained by AP-PCR method

text: Germination stage

text: E.coli JM109

characteristic: lab-host

text: Saccharum hybrid cultivar Co 62175

text: Leaf

text: Sugarcane cv. Co62175 salt stress

text: pTZ T/A cloning vector


text: Northeast Forestry University

text: live

text: Generic

text: Betula platyphylla

characteristic: organism

text: leaf

text: cDNA Library of Betula platyphylla leaf


text: CNRS/EPHE/UPVD

text: live

text: Generic

text: whole body

characteristic: cell type

text: Pocillopora damicornis/Symbiodinium spp. mixed library

text: Bacterial Induced Genes (B-I) SSH library

characteristic: title

text: Plasmid

text: pCR4(r)-TOPO(r) (Invitrogen)


text: CNRS/EPHE/UPVD

text: live

text: Generic

text: whole body

text: Bacterial Repressed Genes (B-R) SSH library

text: Plasmid

text: pCR4(r)-TOPO(r) (Invitrogen)


text: CNRS/EPHE/UPVD

text: live

text: Generic

text: whole body

text: Thermo Bacterial Induced Genes (TB-I) SSH library

text: Plasmid

text: pCR4(r)-TOPO(r) (Invitrogen)


text: CNRS/EPHE/UPVD

text: live

text: Generic

text: whole body

text: Thermo Bacterial Repressed Genes (TB-R) SSH library

text: Plasmid

text: pCR4(r)-TOPO(r) (Invitrogen)


text: Max Planck Institute for Chemical Ecology

text: live

text: Generic

text: Nicrophorus vespilloides SSH; immune-inducible transcriptome analysis

text: adults

characteristic: dev-stage

text: Nicrophorus vespilloides

text: male and female

text: Nic-SSH

text: Plasmid

text: pCRII-TOPO


characteristic: External Id

text: Institute of Plant Biology, University of Wroclaw

text: live

text: Generic

text: var. Krak

text: Specific primers were designed and amplified the sequence via PCR reaction using cDNA as a template.

text: 7 days old seedlings

text: roots

text: Cucumis sativus

text: hermaphrodite

text: roots

characteristic: tissue-type

text: cDNA synthesized from total RNA isolated from cucumber roots