text: Houston Community College Northwest

text: live

text: Generic

text: Recombinant inbred line derived from parents Flip84-92C (Cicer arietinum) and PI489777 (Cicer reticulatum). Plants were grown hydroponically on low Fe (0.5 uM Fe[III]EDDHA) from planting until harvest.

text: 17 day old seedling

text: Cicer arietinum x Cicer reticulatum

text: Root

characteristic: tissue-type

text: HCC-ENGAGE


status: PUBLIC

characteristic: External Id

text: USDA-Agricultural Research Station

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

tag: attribute

characteristic: cultivar

text: SSH library; Verticillium dahliae isolate Ls17- infected plants.

characteristic: description

text: Lactuca sativa

characteristic: organism

text: Leaf

text: Lactuca sativa PI251246 V. dahliae infected leaf

characteristic: title


text: Tokyo University of Marine Science and Technology

text: live

text: Generic

text: Cells isolated from tissue were treated with ConA and LPS. After incubation for several hours, the cells were harvested and used for cDNA library construction.

text: Pagrus major

text: kidney

text: Pagrus major kidney


text: live

text: Generic

text: Paralichthys olivaceus

text: brain

text: Paralichthys olivaceus brain


text: live

text: Generic

text: Paralichthys olivaceus

text: kidney

text: Paralichthys olivaceus kidney


text: live

text: Generic

text: synonym: Loligo bleekeri

text: Heterololigo bleekeri

text: squid liver

text: Loligo bleekeri squid liver


text: live

text: Generic

text: Pagrus major

text: spleen

text: Pagrus major spleen


text: College of Marine Sciences, Shanghai Ocean University

text: live

text: Generic

text: Adult

characteristic: dev-stage

text: Portunus trituberculatus

text: male

characteristic: sex

text: gill

text: swimming crab gill cDNA Library

text: pBluescriptII

characteristic: vector


text: Central Institute of Fisheries Technology

text: live

text: Generic

text: Suppression subtractive hybridization was carried out. The products were then cloned into pTZ57R/T vector

text: Penaeus indicus

text: Hepatopancreas

text: WSSV infected F. indicus SHH Library


text: Universidad de Cordoba

text: live

text: Generic

text: Chandler

text: red fruit

text: E. coli

characteristic: lab-host

text: fruit

characteristic: organ

text: Fragaria x ananassa

text: EcoRI

text: EcoRI

text: achenes and receptacle

text: Fragaria x ananassa var. Chandler sustractive red fruit UCO library C1

text: Plasmid

text: pBS


text: Universidad de Cordoba

text: live

text: Generic

text: Chandler

text: red fruit

text: E .coli

text: fruit

text: Fragaria x ananassa

text: EcoRI

text: EcoRI

text: achenes and receptacle

characteristic: tissue-type

text: Fragaria x ananassa var. Chandler sustractive red fruit UCO library C2

text: Plasmid

text: pBS


characteristic: External Id

text: Universidad de Cordoba

text: live

text: Generic

text: Chandler

text: red fruit

text: E. coli

text: fruit

text: Fragaria x ananassa

text: EcoRI

text: EcoRI

text: achenes and receptacle

text: Fragaria x ananassa var. Chandler red fruit UCO library C3

text: Plasmid

text: pBS


text: University of Massachusetts, Boston

characteristic: INSDC center name

text: live

text: Generic

text: Daphnia magna

text: Daphnia magna microarray consortium pooled libraries


text: Max Planck Institute for Developmental Biology

characteristic: INSDC first public

text: live

text: Generic

text: mixed stage

text: Pristionchus pacificus

text: hermaphrodite

text: California (PS312)

characteristic: strain

text: whole body


text: Northeast Forestry University

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: 3 month after germination

text: Puccinellia tenuiflora

text: Leaf

text: Puccinellia tenuiflora SSH library

text: Plasmid

text: pGEM-T


text: National Institute of Agrobiological Sciences

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: adult

text: Sus scrofa

text: alveolar macrophages

text: full-length enriched swine cDNA library, adult alveolar macrophages


text: live

text: Generic

text: adult

text: Sus scrofa

text: brain (frontal lobe)

text: full-length enriched swine cDNA library, adult brain (frontal lobe)


text: live

text: Generic

text: adult

text: Sus scrofa

text: back fat

text: full-length enriched swine cDNA library, adult back fat


text: live

text: Generic

text: adult

text: Sus scrofa

text: colon

text: full-length enriched swine cDNA library, adult colon


text: live

text: Generic

text: adult

text: Sus scrofa

text: hypothalamus

text: full-length enriched swine cDNA library, adult hypothalamus


text: live

text: Generic

text: adult

text: Sus scrofa

text: kidney

text: full-length enriched swine cDNA library, adult kidney


text: live

text: Generic

text: adult

text: Sus scrofa

text: longissimus

text: full-length enriched swine cDNA library, adult longissimus


text: live

text: Generic

text: adult

text: Sus scrofa

text: prostate

text: full-length enriched swine cDNA library, adult prostate


text: live

text: Generic

text: adult

text: Sus scrofa

text: pituitary gland

text: full-length enriched swine cDNA library, adult pituitary gland


text: live

text: Generic

text: adult

text: Sus scrofa

text: submaxillary gland

text: full-length enriched swine cDNA library, adult submaxillary gland


text: live

text: Generic

text: adult

text: Sus scrofa

text: peripheral blood lymphocytes

text: full-length enriched swine cDNA library, adult peripheral blood lymphocytes


text: live

text: Generic

text: adult

text: Sus scrofa

text: placenta

text: full-length enriched swine cDNA library, adult placenta


text: live

text: Generic

text: dendritic cells (immature)

characteristic: cell type

text: adult

text: Sus scrofa

text: full-length enriched swine cDNA library, adult dendritic cells (immature)


text: live

text: Generic

text: adult

text: Sus scrofa

characteristic: organism

text: small intestine

text: full-length enriched swine cDNA library, adult small intestine


text: live

text: Generic

text: adult

text: Sus scrofa

text: mesenteric lymph nodes

text: full-length enriched swine cDNA library, adult mesenteric lymph nodes

characteristic: title


text: Institute of Biochemistry, Faculty of Medicine

text: live

text: Generic

text: Library was prepared using Clontech PCR-Select cDNA Subtraction Kit (BD Bioscience) starting from total RNA isolated from tumor and adjacent non-tumor gastric tissue. We constructed subtracted cDNA library by hybridizing non-tumor (driver) and tumor (tester) cDNAs, obtaining up-regulated sequences in cancerous tissue. Subtracted library of up-regulated genes was cloned into pCRII plasmids using TA cloning kit (Invitrogen). Sequence information was obtained for all clones.

characteristic: description

text: One Shot Competent Cells

text: stomach

text: Homo sapiens

text: female

text: diffuse (Lauren) gastric adenocarcinoma

text: Human gastric cancer subtracted library

text: Plasmid

text: pCRII


text: Newcastle University

text: live

text: Generic

text: Trophozoite grown in vitro

text: The cDNA library was prepared from Trichomonas vaginalis trophozoites grown in vitro (mid-log phase) using polyA+ purified mRNA and using the ZAP-cDNA synthesis and cloning kit. pBluescript_SK_phagemids were excided and minipreps processed for sequencing - non-normalized library.

text: G3 (clinical isolate)

characteristic: isolate

text: Trichomonas vaginalis

text: EcoRI

text: XhoI

text: non-normalized cDNA library from Trichomonas vaginalis trophozoites grown in vitro (mid-log phase)

text: phagemide excised from Uni-ZAP XR vector


text: Newcastle University

text: live

text: Generic

text: Trophozoite grown in vitro

text: The cDNA library was prepared from Trichomonas vaginalis trophozoites grown in vitro (mid-log phase) using polyA+ purified mRNA and using the ZAP-cDNA synthesis and cloning kit. pBluescript_SK_phagemids were excided and normalized and minipreps processed for sequencing.

text: G3 (clinical isolate)

text: Trichomonas vaginalis

text: EcoRI

text: XhoI

text: normalized cDNA library from Trichomonas vaginalis trophozoites grown in vitro (mid-log phase)

characteristic: vector


text: South China Normal University, College of Life Sciences

text: live

text: Generic

text: 5th to 6th instar molting larvae

characteristic: dev-stage

text: Spodoptera litura

text: midgut

text: Spodoptera litura 5th to 6th instar molting larvae Library


text: live

text: Generic

text: feeding 6th instar larvae

text: Spodoptera litura

text: midgut

text: Spodoptera litura feeding 6th instar larvae Library


text: live

text: Generic

text: prepupae

text: Spodoptera litura

text: midgut

text: Spodoptera litura prepupae Library


text: University of Oregon

text: live

text: Generic

text: Wyeomyia smithii

text: F4 Hybrids

text: Wyeomyia smithii cDNA library


text: Radboud University Nijmegen Medical Centre

text: live

text: Generic

text: Rapid amplification of cDNA ends (5'- and 3'- RACE, Clontech) was performed on Human retina Marathon-Ready (Clontech) cDNA

text: Homo sapiens

text: retina

text: RACE human retina cDNA


text: live

text: Generic

text: Rapid amplification of cDNA ends (5'- and 3'- RACE, Clontech) was performed on Human testis Marathon-Ready (Clontech) cDNA

text: Homo sapiens

text: testis

text: RACE human testis cDNA


text: China Agricultural University

text: live

text: Generic

text: liver

characteristic: organ

text: Gallus gallus

text: male

characteristic: sex

text: liver

text: Male Chicken liver cDNA library


characteristic: External Id

text: Nanjing Agricultural University

text: live

text: Generic

text: Xiahei

characteristic: cultivar

text: Escherichia coli DH5a

characteristic: lab-host

text: Vitis vinifera

text: Flower and Fruit

characteristic: tissue-type

text: Grape cv. Xiahei flower and developing fruit

text: pCR II


text: British Antarctic Survey

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Prepared from six Antarctic krill collected from northwest of South Georgia on 2nd February 2008 at 00:50 hours.

text: Euphausia superba

text: Head

text: 0050 cDNA library

text: pDNR-Lib


text: British Antarctic Survey

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Prepared from six Antarctic krill collected from northwest of South Georgia on 3rd February 2008 at 06:00 hours.

text: Euphausia superba

text: Head

text: 0600 cDNA library

text: pDNR-Lib


text: British Antarctic Survey

text: live

text: Generic

text: Prepared from six Antarctic krill collected from the vicinity of South Orkney Islands on 6th Jan 2008 at 07:00 hours.

text: Euphausia superba

characteristic: organism

text: Head

text: 0700 cDNA library

characteristic: title

text: pDNR-Lib


text: British Antarctic Survey

text: live

text: Generic

text: Prepared from six Antarctic krill collected from the vicinity of South Orkney Islands on 4th Jan 2008 at 14:00 hours.

characteristic: description

text: Euphausia superba

text: 1400 cDNA library

text: pDNR-Lib


text: British Antarctic Survey

text: live

text: Generic

text: Prepared from six Antarctic krill collected from the vicinity of South Orkney Islands on 18th Jan 2008 at 20:30 hours.

text: Euphausia superba

text: 2030 cDNA library

text: pDNR-Lib

characteristic: vector


text: University of Missouri-Columbia

text: live

text: Generic

text: Funding: The production of ESTs submitted in this project was funded by USDA Grant MRI-2002-03476 entitled 'Bovine ESTs: Focus on Female Reproduction' to RS Prather(Primary Investigator), E Antoniou, HA Garverick, JA Green, MC Lucy, RM Roberts, MF Smith and RS Youngquist. Genetic Source: Heifers for the project were purchased from Circle A Ranch, Iberia, MO (http://www.circlearanch.com/home.html). These heifers, while not registered have known Angus pedigrees going back at least 4 generations. Samples collected: The samples consisted of the following: germinal vesicle-stage oocytes; in vitro derived embryos (2-cell, morula, blastocyst and nuclear transfer blastocyst); in vivo blastocysts and conceptuses (days 8, 14, 16 and 18); corpora lutea (days 3, 5, 8, 14, 16, 18 and 35); ovarian follicles (days 0, non-recruited, recruited, early selected and preovulatory); oviduct (days 0, 3 and 5); endometrium (days 5, 8, 14, 16, 18 and 35); and placenta/embryo from day 35 conceptuses. Expanded descriptions of how the tissues were collected can be found at the following URL: http://genome.rnet.missouri.edu/Bovine/Methods.html. Library construction (Standard Protocol): All procedures have been described in detail elsewhere (Soares et al., 1994; Bonaldo et al., 1996; Jiang et al., 2001). Total cellular RNA from each sample was isolated by using STAT-60 reagent (Tel-Test, Friendswood, TX) and the poly(A)+ RNA was obtained by two rounds of purification with the Oligotex mRNA isolation kit (Qiagen) according to the manufacturer's instructions. The libraries were constructed essentially as described by the manufacturer's instructions provided with the SuperScript Plasmid System (Invitrogen, cat. no. 18248-013). Briefly, 1mcg of poly(A)+ RNA was annealed at c37 degrees with 10mcg of NotI-tag-dT18 oligonucleotide (GCTGCTCGCGGCCGC-tag-T18) and reverse transcribed at c37 degrees with SuperScript II (Invitrogen) reverse transcriptase (Jiang et al., 2001). The 'tag' represents a tissue/stage-specific ten-base sequence identifier (http://genome.uiowa.edu/pubsoft/software.html) present in the oligonucleotide used to prime first-strand synthesis. Second strand synthesis was performed with T4 DNA polymerase in the presence of DNA ligase and RNase H. After second strand synthesis, the double-stranded cDNAs were ligated to SalI adapters (Invitrogen-Life Technologies) and digested with NotI. The cDNAs were size selected by passage through cDNA size fractionation columns (Invitrogen-Life technologies). The cDNAs derived from each developmental stage of a particular tissue were mixed on an equimolar basis and ligated directionally into the NotI and SalI sites of the pCMV-SPORT6 vector (Invitrogen). After ligation of the inserts, the plasmids were electroporated into DH10B bacteria. Library Construction (PCR Protocol): The amount of mRNA that was recovered from oocytes and embryos was quite limiting and was not sufficient for library production with the standard protocol. Therefore, PCR-based protocol was utilized for producing libraries from sources in which the amount of extracted mRNA was small (oocytes and embryos). Poly-A RNA was isolated by using the MicroPoly(A) Pure kit from Ambion (cat. # 1918). The mRNA was reverse transcribed with a NotI-tag-dT18 oligonucleotide and a SMART oligonucleotide (Clontech) modified to contain a SalI site to generate full-length cDNA with a sequence complementary to the SMART oligonucleotide. Sequences within the SMART and dT oligonucleotides were used as primers to amplify the cDNAs by PCR with pfu turbo polymerase (Stratagene). The resulting PCR products were purified, digested with NotI and SalI and size fractionated by using Chroma Spin-1000 columns (Clontech). Purified cDNA from each PCR reaction was quantitated and mixed on an equimolar basis for ligation into the pCMV- SPORT6 vector. Preliminary Library Characterization: Randomly chosen clones from each library were analyzed by restriction digestion to determine average insert size (96 clones) and by sequencing (~4 96-well plates) to confirm library quality [e.g. the presence of short polyA+ tails, genomic DNA contamination (must be <1%), ribosomal RNA clones (must be <1%), etc.] and to provide a sequence database representing the predominant clones in each library. The clones were sequenced at the University of Missouri-Columbia DNA Core Facility. After production of the libraries, equal numbers of recombinants from each library were pooled to produce a single mixed library (mega-library) for more extensive sequencing. Bioinformatics work was performed by GK Springer's bioinformatics group in Computer Science at the University of Missouri-Columbia. Clone Requests: Requests for clones should be made to the Director of the University of Missouri DNA Core facility at: bovine@rnet.missouri.edu. Bonaldo MF, Lennon G, Soares MB,. Normalization and Subtraction: Two approaches to facilitate gene discovery. Genome Res, 1996; 6:791-806. Jiang H, Bivens NJ, Ries JE, Whitworth KM, Green JA, Forrester LJ, Springer GK, Didion BA, Mathialagan N, Prather RS, Lucy MC (2001) Constructing cDNA libraries with fewer clones that contain long poly(dA) tails. Biotechniques 31:38-42. Soares MB, MF Banaldo, P Jelene, L Su, L Lawton, A Efstrantiadis. 1994. Construction and characterization of a normalized cDNA library. Proc Natl Acad Sci, 91:9228-9232.

text: Day 35 Placenta

characteristic: dev-stage

text: Bos taurus

text: Cloning


text: Universidad de Oviedo

text: live

text: Generic

text: Sequences obtained by GeneSnare, and cloned into p-GemT

text: leaf

characteristic: organ

text: Jatropha curcas

text: leaf

text: Jc-Drought

text: p-GEMT


text: The University of Tokyo

text: live

text: Generic

text: country: Japan:Hokkaido

text: adult

text: Delia antiqua

text: male and female

characteristic: sex

text: antenna

text: Delia antiqua antenna adult


characteristic: External Id

text: Universite Claude Bernard Lyon 1

text: live

text: Generic

text: isolation_source: poor sandy soil from a Pinus sylvestris forest stand; ancient industrial site polluted with metals from a pyrometallurgical zinc smelter ; country: Belgium:North East Belgium, Campine, Lommel, Province of Limburg ; environmental_sample; lat_lon: 51.14 N 5.15 E ; collection_date: 11-Nov-2006 ; collected_by: Marmeisse R, Fraissinet-Tachet L, Lehembre F.

text: uncultured eukaryote

text: uncultured eukaryote from poor sandy soil, zinc smelter, Lommel


text: Institute of Biochemistry, Faculty of Medicine

text: live

text: Generic

text: Library was prepared using Clontech PCR-Select cDNA Subtraction Kit (BD Bioscience) starting from total RNA isolated from tumor and adjacent non-tumor gastric tissue. We constructed subtracted cDNA library by hybridizing tumor (driver) and normal (tester) cDNAs, obtaining down-regulated sequences in cancerous tissue. Subtracted library of down-regulated genes was cloned into pCRII plasmids using TA cloning kit (Invitrogen). Sequence information was obtained for all clones.

text: One Shot Competent Cells

text: stomach

text: Homo sapiens

text: female

text: diffuse (Lauren) gastric adenocarcinoma

text: Human gastric cancer subtracted, down-regulated, library

text: Plasmid

text: pCRII


text: live

text: Generic

text: Library was prepared using Clontech PCR-Select cDNA Subtraction Kit (BD Bioscience) starting from total RNA isolated from stomach tissue with chronic gastritis and adjacent non-affected gastric tissue. We constructed subtracted cDNA library by hybridizing non-affected (driver) and chronic gatritis (tester) cDNAs, obtaining up-regulated sequences in chronic gastritis tissue. Subtracted library of up-regulated genes was cloned into pCRII plasmids using TA cloning kit (Invitrogen). Sequence information was obtained for all clones.

text: One Shot Competent Cells

text: stomach

text: Homo sapiens

text: Chronic gastritis subtracted, up-regulated, library

text: Plasmid

text: pCRII


text: live

text: Generic

text: Library was prepared using Clontech PCR-Select cDNA Subtraction Kit (BD Bioscience) starting from total RNA isolated from stomach tissue with chronic gastritis and adjacent non-affected gastric tissue. We constructed subtracted cDNA library by hybridizing chronic gastritis (driver) and non-affected (tester) cDNAs, obtaining down-regulated sequences in chronic gastritis tissue. Subtracted library of down-regulated genes was cloned into pCRII plasmids using TA cloning kit (Invitrogen). Sequence information was obtained for all clones.

text: One Shot Competent Cells

text: stomach

text: Homo sapiens

text: Chronic gastritis subtracted, down-regulated, library

text: Plasmid

text: pCRII


text: University of Kentucky

text: live

text: Generic

text: RNA extracted from whole animals, or from specific tissues was used to construct cDNA libraries from which ESTs were generated.

text: mixed, larval, adult

text: E.coli

text: Aphis gossypii

text: mixed tissues, whole insects, midgut, nervous system

text: Aphis gossypii cDNA Libraries

text: plasmid


text: Norwegian School of Veterinary Science

text: live

text: Generic

text: Salmo salar

text: Salmo salar intestine


text: University of Agricultural Sciences, Dharwad

text: live

text: Generic

text: E 36_1

characteristic: cultivar

text: seedling

text: Sorghum bicolor

text: water-stressed shoot

text: Sorghum bicolor water-stressed shoot seedling


text: Balikesir University

text: live

text: Generic

text: Ayvalik

text: Olea europaea

text: leaves in July and in November

text: Olea europaea cv. Ayvalik leaves in July and in November


text: Radboud University Nijmegen Medical Centre

text: live

text: Generic

text: RT-PCR was performed on Human retina Marathon-Ready (Clontech) cDNA

text: Homo sapiens

text: retina

text: RT-PCR human retina cDNA


text: live

text: Generic

text: RT-PCR was performed on Human retina Marathon-Ready (Clontech) cDNA and and on Human testis Marathon-Ready (Clontech) cDNA

text: Homo sapiens

text: retina and testis

text: RT-PCR human retina and testis cDNA


text: National Bureau of Animal Genetics Resources

text: live

text: Generic

text: cDNA subtracted library was prepared from lactating vs non-lactating mammary gland tissue.

text: Bubalus bubalis

text: mammary gland

text: Subtracted cDNA library from buffalo mammary gland


text: Norwegian University of Science and Tecnology

text: live

text: Generic

text: larvae day 17 post-fertilization

text: Gadus morhua

text: larvae

text: Gadus morhua larvae SSH library enriched for genes up-regulated by the presence of bacteria


text: live

text: Generic

text: larvae day 17 post-fertilization

text: Gadus morhua

text: larvae

text: Gadus morhua larvae SSH library enriched for genes down-regulated by the presence of bacteria


text: Turku Centre for Biotechnology

text: live

text: Generic

text: cDNA library was constructed from whole Merlin's grass collected from lake of Kustavi located in Southern Finland.

text: Isoetes lacustris

text: EcoRI

text: XhoI

text: Merlin's grass library

text: pBluescript SK(-)


text: Turku Centre for Biotechnology

text: live

text: Generic

text: cDNA library was constructed from the emerging fiddlehead of bracken fern. The fiddlehead of Pteridium aquilinum was harvested from Kaarina located in South West Finland.

text: Pteridium aquilinum

text: EcoRI

text: XhoI

text: Emerging fiddlehead

text: Fern fiddlehead library

text: pBluescript SK(-)


text: Helmholtz-Zentrum fur Umweltforschung Leipzig

text: live

text: Generic

text: pcr, gel extraktion, ligation into pCR2.1, transformation to E. coli top 10, plasmid isolation, sequencing with M13

text: E. coli One Shot TOP10

characteristic: lab-host

text: Scenedesmus vacuolatus

text: SAG 211-8b

characteristic: strain

text: EST-Scenedesmus vacuolatus

text: Plasmid


text: Universidad de Malaga

characteristic: INSDC center name

text: live

text: Generic

text: Root

text: Pinus pinaster

text: Ammonium Responsive Genes

text: pGEMT


text: Universidade Estadual de Campinas (UNICAMP)

characteristic: INSDC first public

text: live

text: Generic

text: Library was prepared from total RNA based on the Gateway Technology (CloneMiner cDNA Library Construction Kit. Invitrogen 182 49-029).

text: Venom gland

text: Bothrops alternatus

text: Bothrops alternatus venom gland

text: Plasmid

text: pDONR 222


text: University of Sydney

characteristic: INSDC last update

text: live

text: Generic

text: Kiev mutant

text: Lupinus albus

text: Phloem exudate

characteristic: tissue-type

text: Lupinus albus phloem cDNA library


text: Sichuan Agricultural University

characteristic: INSDC secondary accession

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: SRA accession

text: Intestinal tract

text: Monopterus albus

text: Male

text: Ecoli DH10B

text: Intestinal tract tissue cDNA library of Monopterus albus


text: Uppsala University

text: live

text: Generic

characteristic: NCBI submission package

text: Spironucleus barkhanus isolated from the gall bladder of the freshwater salmonid grayling (Thymallus thymallus) in Glomma River, south-eastern Norway, 2006. Exponentially growing S. barkhanus were harvested at passage 13. Following the isolation of mRNA using Poly(A)PuristTM MAG system (Ambion) directed and size-fractionated cDNA libraries was made using the CloneMinerTM cDNA Library Construction Kit (Invitrogen).

characteristic: description

text: Spironucleus barkhanus

characteristic: organism

text: S. barkhanus cloneMiner cDNA library

characteristic: title


text: National Central University

text: live

text: Generic

text: Lilium formosanum

text: flower bud

text: Lilium formosanum flower buds library


text: University of Maryland - College Park

text: live

text: Generic

text: Culture harvested from various time points during the day and across the life cycle. No sexual stage was seen for this strain.

text: all except for sexual stages

characteristic: dev-stage

text: Coleochaete orbicularis

text: UTEX 2651

text: Coleochaete orbicularis EST library


characteristic: External Id

text: University of Maryland - College Park

text: live

text: Generic

text: Culture harvested from various time points during the day and across the life cycle.

text: all stages

text: Spirogyra pratensis

text: UTEX 921

text: Spirogyra pratensis EST library


text: Max Planck Institute for Plant Breeding Research

text: live

text: Generic

text: primary hyphae

characteristic: cell type

text: biotrophic infection

text: Colletotrichum higginsianum

text: Colletotrichum higginsianum IMI349063A biotrophic infection primary hyphae


text: Tsinghua University

text: live

text: Generic

text: D-shape and Trochophore

text: Pinctada fucata

text: suppression subtractive hybridization library of pinctada fucata D-shape and Trochophore

text: pGEM-T Easy Vector

characteristic: vector


text: Tsinghua University

text: live

text: Generic

text: Umbo and Infant

text: Pinctada fucata

text: suppression subtractive hybridization library of pinctada fucata Umbo and Infant oyster

text: pGEM-T Easy Vector


text: Tsinghua University

text: live

text: Generic

text: Trochophore and Umbo

text: Pinctada fucata

text: suppression subtractive hybridization library of pinctada fucata Trochophore and Umbo

text: pGEM-T Easy Vector


text: Zhejiang Wanli University

text: live

text: Generic

text: Tegillarca granosa

text: hamecyte

text: Tegillarca granosa hamecyte library


text: National University of Singapore

text: live

text: Generic

text: Suppression subtractive Hybridization.

text: Liver

characteristic: organ

text: Protopterus annectens

text: Liver

text: P. annectens normoxia and hypoxia SSH library


text: Indian Institute of Vegetable Research

characteristic: INSDC center name

text: live

text: Generic

characteristic: cultivar

text: Total RNA was isolated using TRIZOL method from the leaf tissues collected after 24 hrs of inoculation with early blight pathogen (Alternaria solani). cDNA was prepared using SuperScript Plasmid System for cDNA Synthesis with Gateway (Invitrogen) cloned in pCMV.SPORT6 cloning vector, and transformed in XL1 Blue strain of E.coli.

text: E.coli (XL1 Blue)

characteristic: lab-host

text: Solanum habrochaites

text: Mlu I

text: Kpn I

text: Hermaphrodite

characteristic: sex

text: Leaf

text: Tomato Early Blight resistance EST

text: Plasmid


text: HKUST

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Jatropha curcas

text: Flower and Seed

characteristic: tissue-type

text: Jatropha curcas flower and seed


text: Yokohama National University

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: HiCEP peak; country: Japan: Yamashina, Fujiyoshida

text: 25-28 days old

text: Folsomia candida

text: parthogenetic female

text: Fuji-Japan

characteristic: strain

text: whole body

text: Folsomia candida whole body Fuji-Japan 25-28 days old


text: University of Victoria

text: live

text: Generic

characteristic: NCBI submission package

text: Tissue contributors: John Burka, Department of Biomedical Science, University of P.E.I.

characteristic: description

text: mixed tissue

text: Lepeophtheirus salmonis

characteristic: organism

text: mixed tissue

text: lsaAevu mixed_tissue Lepeophtheirus salmonis (Atlantic Canada) cDNA


characteristic: External Id

text: University of Victoria

text: live

text: Generic

text: Tissue contributors: Frank Nilsen/Heidi Kongshaug, University of Bergen, Norway

text: mixed tissue

text: Lepeophtheirus salmonis

text: mixed tissue

text: lsaAevv mixed_tissue Lepeophtheirus salmonis (Atlantic Norway) cDNA

characteristic: title


text: University of Victoria

text: live

text: Generic

text: Tissue contributors: S. Jones (Fisheries and Oceans Canada)

text: whole

text: Lepeophtheirus salmonis

text: whole

text: lsalevj whole Lepeophtheirus salmonis (Pacific) cDNA

characteristic: vector


text: Northeast Forestry University

text: live

text: Generic

text: Ripe

characteristic: dev-stage

text: E.coli

text: Vitis amurensis

text: Female

text: Pericarp

text: Vitis amurensis Rupr ripe peel Library

text: pDNR-LIB


text: Northeast Forestry University

characteristic: INSDC center name

text: live

text: Generic

text: Veraison

text: E.coli

text: Vitis amurensis

text: Female

text: Pericarp

text: Vitis amurensis Rupr veraison peel Library

text: pDNR-LIB


text: Colegio de Postgraduados campus Campeche

text: live

text: Generic

text: var Azul Weber

characteristic: cultivar

text: Tissue: Tip and basal portion of fully expanded green leaves; and inmature yellow leaves rolled into the central cone of the plant; collected at 4:00 AM, 3:00 PM, and 10:00 PM. Poliadenylated mRNA purified with magnetic Dynabeads from total RNA, previously extracted with Trizol reagent, was used to synthetize cDNAs with SuperScript II. The cDNAs were cloned with Cloneminer kit. The used methods are carefully described in the cited publication.

text: Plants of one and five years old

text: Leaves

characteristic: organ

text: Agave tequilana

text: BsrG I

text: BsrG I

text: mixed

text: aamhphhoc

text: Plasmid


text: live

text: Generic

text: var Azul Weber

text: Poliadenylated mRNA purified with magnetic Dynabeads from total RNA, previously extracted with Trizol reagent, was used to synthetize cDNAs with SuperScript II. The cDNAs were cloned with Cloneminer kit. The used methods are carefully described in the cited publication.

text: Plants in initiation of flower development (floral stems of 1.5 m). Floral stem tips were collected during day (10:00 AM) and night (4:00 AM)

characteristic: lab-host

text: Floral stem tip

text: Agave tequilana

text: BsrG I

text: BsrG I

text: Inflorescence meristem, axilar meristems for umbel ramifications; vascular and photosynthetic tissues of floral stem tip

text: aamhmqmeq

text: Plasmid


text: Universidade Federal do Para

characteristic: INSDC first public

text: live

text: Generic

text: Bragantina

text: SSH library was made using roots of Piper nigrum plants (cultivar Bragantina) inoculated with Fusarium solani f. sp. piperis as tester and non-inoculated plants as driver to identify genes related to defense response.

text: Root

text: Piper nigrum

text: Piper nigrum cv. Bragantina root cDNA library

text: Plasmid

text: pGEM-TEasy


text: Australian Institute of Marine Science

characteristic: INSDC last update

text: live

text: Generic

text: Rhopaloeides odorabile

text: Heat stressed Rhopaloeides odorabile library


text: Eotvos Lorand University

text: live

text: Generic

text: Jemalong 5

text: 21 days

text: Medicago truncatula

text: root

characteristic: tissue-type

text: MtBC (Paradi)


text: China West Normal University

text: live

characteristic: INSDC status

text: Generic

text: Chuanmai 28

text: The subsidiary pistil primordium differentiative phase

text: young spike

text: Triticum aestivum

text: SSH cDNA library of T. aestivum cv. Chuanmai 28 Three pistils mutation

text: pGEM-T


text: China West Normal University

text: live

text: Generic

characteristic: NCBI submission model

text: Chinese Spring

text: young spike

text: Triticum aestivum

text: SSH cDNA library of T. aestivum cv. Chinese Spring Three pistils mutation

text: pGEM-T


text: Anhui Agricultural University

text: live

text: Generic

text: YJ63X and YJ63Y

characteristic: cell-line

text: Camellia sinensis

text: hermaphrodite

characteristic: sex

text: callus

text: cDNA-AFLP of tea (Camellia sinensis L.) callus with different catechins contents

text: pMD18-T Simple


text: Zhejiang Ocean University

text: live

text: Generic

characteristic: NCBI submission package

text: Miichthys miiuy

text: Mimi-mix

text: pDNR-LIB


text: University of Florida

text: live

text: Generic

text: Georgia Green

text: leaf

text: Arachis hypogaea

characteristic: organism

text: phorate responsive differentially displayed cDNA clones


characteristic: External Id

text: Natural Resources Canada

text: live

text: Generic

text: resting and germinating urediniospores, germ tubes, epiphytic mycelia, etc.

characteristic: cell type

text: Naturally infected leaves were collected in September. Epiphytic materials corresponding to resting and germinating uredinospores, germ tubes, epiphytic mycelia and uredinia were scraped and characterized by qPCR to ensure there was no cross-contamination (Boyle et al. 2005. Appl. Environ. Microbiol. 71, 1546-1552; RNA was obtained by grinding material with a Mixer Mill MM300; mRNA purification using Dynabeads; cDNA preparation using Stratagene pBluescript II XR cDNA library construction kit.

characteristic: description

text: epiphytic materials at different growth stages scraped from infected poplar (Populus deltoides)

text: Natural field infection

characteristic: isolate

text: E. coli ElectroMAXTM DH10BTM

text: Melampsora medusae f. sp. deltoidis

text: EcoRI (5'-end)

text: XhoI (3'-end)

text: MMD-E

text: pBluescript II XR


text: Natural Resources Canada

characteristic: INSDC center name

text: live

text: Generic

text: Isolate MMT Lotb. 157. In vitro infected leaves were collected 13 days afer inoculation. Epiphytic materials corresponding to resting and germinating uredinospores, germ tubes, epiphytic mycelia and uredinia were scraped; RNA was obtained by grinding material with a Mixer Mill MM300; mRNA purification using Dynabeads; cDNA preparation using Clontech SMART cDNA library construction kit.

text: epiphytic materials at 13 days after inoculation scraped from infected poplar (Populus tremuloides)

characteristic: dev-stage

text: MMT Lotb. 157

text: E. coli ElectroMAXTM DH10BTM

text: Melampsora medusae f. sp. tremuloides

text: SfiI

text: MMT-E

characteristic: title

text: pDNR-LIB

characteristic: vector


text: Natural Resources Canada

characteristic: INSDC first public

text: live

text: Generic

text: Isolate MO Cal01. In vitro infected leaves were collected 13 days afer inoculation. Epiphytic materials corresponding to resting and germinating uredinospores, germ tubes, epiphytic mycelia and uredinia were scraped; RNA was obtained by grinding material with a Mixer Mill MM300; mRNA purification using Dynabeads; cDNA preparation using Clontech SMART cDNA library construction kit.

text: epiphytic materials at 13 days after inoculation scraped from infected poplar (Populus trichocarpa)

text: MO Cal01

text: E. coli ElectroMAXTM DH10BTM

characteristic: lab-host

text: Melampsora occidentalis

text: SfiI

text: MO-E

text: pDNR-LIB


text: Div. of Crop Protection, Central Research Institute for Jute and Allied Fibres

characteristic: INSDC last update

text: live

text: Generic

characteristic: cultivar

text: A SSH library of mesta against MeYVMV infection. Mesta plants were raised from HC-583 seeds under glasshouse condition and whitefly transmission was carried out with 11 days old plants. Total RNA were isolated from emerging leaves collected from infected plants as well as from control plants after 12 hr, 24 hr, 2 and 4 dpi using Trizol reagent (invitrogen). mRNA was isolated by mRNA isolation kit (Roche Applied Science). The differentially regulated subtracted cDNA pool in infected plants were obtained using the Clontech PCR select Subtraction Kit. This subtracted cDNA pool were then cloned in pJET vector and transformed in DH5-alpha cells and thus differentially regulated cDNA library was constructed.

text: leaves from 12 days old plants of mesta (HC-583).

text: E. coli DH5alpha

text: Hibiscus cannabinus

text: Leaves

characteristic: tissue-type

text: Library of Mesta plant infected with Mesta yellow vein mosaic virus

text: pJET


text: College of life Sciences, South-Central University for Nationalities

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: developing seeds

text: E coli. DH10B

text: seeds

characteristic: organ

text: Arachis hypogaea

characteristic: strain

text: seeds

text: Arachis hypogaea strain 063103 developing seeds

text: pDNR-LIB Donor Vector from CLONTECH


characteristic: External Id

text: Universiti Putra Malaysia

text: live

text: Generic

characteristic: NCBI submission package

text: Mimi Palmer

text: Flowers at different developmental stages collected at different time points in a 24-hour cycle.

text: bud; half-open flower; full-open flower

text: Flower

text: Vanda hybrid cultivar

characteristic: organism

text: Flower

text: Vanda Mimi Palmer Floral cDNA library


text: Institute of Life Sciences

text: live

text: Generic

text: Suppressive subtraction hybridization was performed using the SuperSMART cDNA Synthesis Kit and the Clontech PCR-Select cDNA Subtraction Kit taking the mRNA from the internode as Tester and that from leaves as Driver. The secondary PCR product was non-directionally cloned in a pGEM-T easy vector (Promega) using PCR Cloning Kit. The EST clones were sequenced using the M13 Forward primer at Chromous Biotech.

characteristic: description

text: fully developed plants, grown in natural condition

text: Bambusa balcooa

text: ECoRI

text: ECoRI

text: internode

text: Forward library from PCR-based SSH of cDNA isolated from internode and leaf tissue

text: Plasmid

text: pGEM-T easy


text: University of Florida

characteristic: INSDC center name

text: live

text: Generic

text: Total RNA was isolated and cDNA synthesized. Adaptors were ligated onto the driver and tester cDNA. The driver seauences were subtracted from the tester. The product was cloned and traditional Sanger sequencing generated.

text: Adult

text: Citrus japonica var. margarita

text: leaf

text: Fortunella margarita adult leaf subtraction library


text: United Planters' Association of Southern India-Tea Research Foundation, Tea Research Institute

text: live

text: Generic

text: Creator SMART cDNA library construction kit (Clonetech)

text: Camellia sinensis

text: Bud

text: cDNA library from dormant bud of Camellia sinensis

text: Plasmid


text: College of Agronomy and Biotechnology, China Agricultural University

text: live

text: Generic

text: Zhong G5

text: Gossypium hirsutum

text: root

text: Cotton root NaCl treated suppression subtractive hybridization cDNA library


text: Huazhong Agricultural University

text: live

text: Generic

text: Suppression subtractive hybridization (SSH) library.

text: Boehmeria clidemioides var. diffusa

text: SSH library of mRNA induced by ramie anthracnose


text: Jaypee Institute of Information Technology

text: live

text: Generic

text: The cDNA was isolated from 72 hours embryonic chick cardiac cDNA expression library.

text: embryonic

characteristic: dev-stage

text: Gallus gallus

text: cardiac


text: Boyce Thompson Institute for Plant Research

text: live

text: Generic

text: The VIGS library was derived from Nicotiana benthamiana leaf tissues exposed to various biotic and abiotic elicitors (del Pozo et al., 2004, EMBO J 23:3072-3082).

text: Nicotiana benthamiana

text: Leaf tissue

text: Virus-induced gene silencing (VIGS) library from Nicotiana benthamiana

characteristic: title


text: live

text: Generic

text: Tomato genes whose expression was induced during defense responses to Pseudomonas syringae pv. tomato were identified from a previous transcription profiling study (Mysore et al., 2002, Plant J 32:299-315). Gene fragments from several of these immunity-induced genes were cloned into a TRV-based vector. These were used for virus-induced gene silencing (VIGS) in Nicotiana benthamiana.

text: Solanum lycopersicum

text: Leaf tissue

text: Immunity-induced gene collection from Solanum lycopersicum (tomato)


text: MPI for Molecular Genetics

text: live

text: Generic

text: This sequence is believed to represent human contamination in the clone library. Total RNA was extracted from 10 goldfish specimens of both sex. Fish olfactory epithelia were unilaterally exposed to copper sulfate for 10 min, and tissue from both sides was collected 3 days later. Collected tissue frozen in liq N2 and stored at -80C prior to RNA extraction. Subtractive library construction was performed with PCR-Select cDNA Subtraction Kit (Clontech, Cat. No. 637401). Unexposed olfactory epithelium tissue was used as a driver. Sequencing was performed at the MPI of Molecular Genetics Berlin.

text: Homo sapiens

text: Human sequence in Carassius auratus library GFSF


text: South African Sugar Association Experiment Station

text: live

text: Generic

text: mRNA was prepared from the leaf roll of the commercial sugarcane variety Nco 376. cDNA was synthesised using standard protocols and ligated to an annealed amplification adaptor set. cDNA was then PCR amplified. Amplified cDNA was ligated and cloned into the Eco RI site of the Lambda ZAP II phage vector.

text: Saccharum hybrid cultivar NCo 376

text: EcoRI

text: leaf roll

text: Sugarcane leaf roll

text: Phage

text: Lambda ZAP II

characteristic: vector


text: National Veterinary Research and Quarantine Services

characteristic: INSDC first public

text: live

text: Generic

text: Differentially expressed gene during the stage conversion of Toxoplasma gondii (ATCC 50174) from tachyzoite to bradyzoite was obtained from annealing control primer (ACP)-based PCR technique.

text: tachyzoite

text: Toxoplasma gondii

text: Differentially Expressed Gene in tachyzoite stage of Toxoplasma gondii (ATCC 50174)

text: TOPO TA


text: live

text: Generic

text: bradyzoite

text: Toxoplasma gondii

text: Differentially Expressed Gene in bradyzoite stage of Toxoplasma gondii (ATCC 50174)

text: TOPO TA


text: live

text: Generic

text: Differentially expressed gene in the tachyzoite stage of Toxoplasma gondii (ATCC 50174) was obtained from annealing control primer (ACP)-based PCR technique. The cDNA fragment was cloned into TOPO TA cloning vector. In vitro Vero cultured.

text: tachyzoite

text: Toxoplasma gondii

text: Differentially Expressed Gene in tachyzoite stage of Toxoplasma gondii (ATCC 50174) (Vero cultured)

text: TOPO TA


text: live

text: Generic

text: Differentially expressed gene in the tachyzoite stage of Toxoplasma gondii (ATCC 50174) was obtained from annealing control primer (ACP)-based PCR technique. The cDNA fragment was cloned into TOPO TA cloning vector. In vivo - infected mouse.

text: tachyzoite

text: Toxoplasma gondii

text: Differentially Expressed Gene in tachyzoite stage of Toxoplasma gondii (ATCC 50174) (Infected mouse)

text: TOPO TA


text: Institute of Hydrobiology, Chinese Academy of Sciences

text: live

text: Generic

text: liver

text: Oncomelania hupensis

text: cDNA library of Oncomelania hupensis liver

text: Plasmid


text: McGill University, Macdonald Campus

text: live

text: Generic

text: This sequence is derived from a suppressive subtraction cDNA library enriched in genes from arctic Oxytropis species. cDNA was synthesized (using SuperSmart PCR cDNA Synthesis kit, Clontech) from mRNA extracted from arctic Oxytropis arctobia and O. maydelliana plantlets grown on petri plates in arctic controlled conditions. Subtraction was carried out using the PCR-select cDNA subtraction kit (Clontech) and cDNAs from temperate O. campestris johannensis and O. splendens plantlets grown on petri plates in temperate controlled conditions. The remaining cDNAs were non-directionally inserted into pCR2.1TOPO vector (Invitrogen) and used to transform ElectroMAX DH10B competent cells (Invitrogen).

text: two leaves

text: whole plantlet

text: Oxytropis arctobia/Oxytropis maydelliana mixed EST library

text: whole plantlet


text: McGill University, Macdonald Campus

text: live

text: Generic

text: This sequence is derived from a suppressive subtraction cDNA library enriched in genes from temperate Oxytropis species. cDNA was synthesized (using SuperSmart PCR cDNA Synthesis kit, Clontech) from mRNA extracted from temperate Oxytropis campestris and O. splendens plantlets grown on petri plates in temperate controlled conditions. Subtraction was carried out using the PCR-select cDNA subtraction kit (Clontech) and cDNAs from arctic Oxytropis arctobia and O. maydelliana plantlets grown on petri plates in arctic controlled conditions. The remaining cDNAs were non-directionally inserted into pCR2.1TOPO vector (Invitrogen) and used to transform ElectroMAX DH10B competent cells (Invitrogen).

text: two leaves

text: whole plantlet

text: Oxytropis campestris/Oxytropis splendens mixed EST library

text: whole plantlet


text: Second Military Medical University

text: live

text: Generic

text: Carthamus tinctorius

text: flower

text: Carthamus tinctorius L. predicted protein mRNA


text: Central Institute of Medicinal and Aromatic Plants

text: live

text: Generic

text: Sampada

text: seedling

text: Papaver somniferum

text: Subtracted cDNA library of salt stress inducible ESTs in Papaver somniferum


text: University of Aberdeen

text: live

text: Generic

text: Tissue samples were collected from wild red grouse and stored in RNA later. Total RNA was extracted using the Trizol PLUS protocol (Invitrogen) and quality checked using the Agilent Bioanalyzer. SMART cDNA was used to construct the library following the Clontech BD PCR-Select cDNA Subtraction kit protocol.

text: Lagopus lagopus scotica

text: Male

characteristic: sex

text: Liver

text: Plasmid

text: pCR2.1 (Invitrogen)


text: University of Aberdeen

characteristic: INSDC last update

text: live

text: Generic

text: Lagopus lagopus scotica

text: Male

text: Spleen

text: Plasmid

text: pCR2.1 (Invitrogen)


text: University of Aberdeen

text: live

text: Generic

text: Lagopus lagopus scotica

text: Male

text: Caecum

text: Plasmid

text: pCR2.1 (Invitrogen)


text: University of Aberdeen

text: live

text: Generic

text: Tissue samples were collected from wild red grouse and stored in RNA later. Total RNA was extracted using the Trizol PLUS protocol (Invitrogen) and quality checked using the Agilent Bioanalyzer. The SMART cDNA Library Construction kit (Clontech) was used to make the library.

text: Lagopus lagopus scotica

text: Male

text: Liver

text: Phagemid

text: LambdaTriplEx2 Vector


text: University of Aberdeen

text: live

text: Generic

text: Lagopus lagopus scotica

text: Male

text: Spleen

text: Phagemid

text: LambdaTriplEx2 Vector


text: University of Aberdeen

text: live

text: Generic

text: Lagopus lagopus scotica

text: Male

text: Caecum

text: Phagemid

text: LambdaTriplEx2 Vector


text: University Medical Center of the Johannes Gutenberg-University Mainz

text: live

text: Generic

text: mRNA purified using the Dynabeads mRNA Purification Kit (Invitrogen), cDNA libraries constructed using the Clone Minor Library construction Kit (Invitrogen, pDONR222), 5' sequencing on an ABI3730XL. Funded by the German Research Foundation (DFG) Priority Program SPP1174 'Deep Metazoan Phylogeny', Project Wi 2116/2.

text: Crateromorpha meyeri

text: choanosome

text: Crateromorpha meyeri DMP cDNA Library


text: live

text: Generic

text: Lubomirskia baikalensis

text: choanosome

text: Lubomirskia baicalensis DMP cDNA Library


text: live

text: Generic

text: Pachydictyum globosum

text: choanosome

text: Pachydictyum globosum DMP cDNA Library


text: College of Animal Science and Technology, Yangzhou University

text: live

text: Generic

text: livers were from Landes geese after 20 days of overfeeding, the profile of genes transcriptional expression in goose fatty liver and control liver were examined by suppression subtractive hybridization (SSH).

text: Anser anser

text: male

text: Landes breed

text: liver

text: Landes goose fatty liver Library


text: Purdue University

text: live

text: Generic

text: Juglans nigra

text: Black walnut ESTs


text: Kyoto Prefectural University

text: live

text: Generic

text: Light Pink Barbara

characteristic: cultivar

text: flower opening

text: Dianthus caryophyllus

text: petal

text: cDNA subtruction library of carnation petals in an opening period


text: Kyoto Prefectural University

text: live

text: Generic

text: Light Pink Barbara

text: flower opening

text: Dianthus caryophyllus

text: petal

characteristic: tissue-type

text: cDNA subtruction library of carnation petals in an early elongation period


text: University of British Columbia

text: live

characteristic: INSDC status

text: Generic

text: Natural population of interior British Columbia

text: The stems of three year old lodgepole pines were inoculated with Ophiostoma clavigerum fungus. For inoculation saplings were wounded with a 3mm deep puncture by sterile needle at the lower part of the stem corresponding to the first year of growth in three year old seedlings with approximate length of treated stem of 10-12 cm. Four wounds were introduced on each of the two opposing sides of the stems. The wounds were inoculated with Ophiostoma clavigerum. Bark tissue, with phloem attached, was harvested at 6 hrs, 2, and 14 days after treatment initiation (four trees per time point). The same amounts of RNA from each sample were pooled for library construction. cDNA was prepared from 5 micrograms of mRNA and directionally ligated into the pBluescript II SK+ vector using Stratagene's pBluescript II XR cDNA Library Construction kit according to manufacturer's instructions, with modifications. Plasmid DNA was then transformed by electroporation into DH10BT1 cells (Invitrogen).

text: E. coli DH10BT1

characteristic: lab-host

text: Pinus contorta

text: EcoRI (5' end of the cDNA)

text: XhoI (3' end of the cDNA)

text: hermaphrodite

text: Bark (phloem and cambium attached) with O. clavigerum inoculation treatment

text: Plasmid

text: pBluescript II SK+


text: University of British Columbia

text: live

text: Generic

characteristic: NCBI submission model

text: Cortical resin ducts were dissected using a laser microdissection system from methyl jasmonate induced 2 year old white spruce PG-653 seedlings. Three seedlings were harvested from 2 day, 4 day and 8 day induction time points. Total RNA was isolated from cortical resin ducts located over 4 (~5 mm) stem sections spanning the leader and 2nd year's growth. Non-normalized cDNA library was produced using Clontech's Creator Smart kit and Evrogen's Trimmer Direct kit primers. cDNA was ligated into pAL17.3 vector, then transformed by electroporation into DH10B-T1 phage resistant cells (Invitrogen).

text: E. coli DH10B

text: Picea glauca

text: Sfi I

text: Sfi I

text: hermaphrodite

text: Cortical resin ducts

text: Plasmid


text: University of British Columbia

text: live

text: Generic

text: Cambium tissue was dissected using a laser microdissection system from methyl jasmonate induced 2 year old white spruce PG-653 seedlings. Three seedlings were harvested from 2 day, 4 day and 8 day induction time points. Total RNA was isolated from cambium tissue located over 4 (~5 mm) stem sections spanning the leader and 2nd year's growth. Non-normalized cDNA library was produced using Clontech's Creator Smart kit and Evrogen's Trimmer Direct kit primers. cDNA was ligated into pAL17.3 vector, then transformed by electroporation into DH10B-T1 phage resistant cells (Invitrogen).

text: E. coli DH10B

text: Picea glauca

text: Sfi I

text: Sfi I

text: hermaphrodite

text: Cambium

text: Plasmid


text: University of British Columbia

text: live

text: Generic

text: Jack pine seedlings were provided by Mike Bendzsak (Forest First, SK) in January 2009. Seed originated from the 'Prince Albert West' seedlot in western Saskatchewan, and was initially grown at the PRT Armstrong Nursery field site. The seedlings were one year old when received at the University of Alberta. Seedlings were transplanted to 3.78 L plastic pots filled with Sunshine Mix #4 (Sun Gro Horticulture Canada TM) growing media. The plants were watered every two to three days in a growth chamber under the following conditions: 19oC (day/night) temperature, relative humidity of 20-35% and under full spectrum fluorescent lamps (200 micromol PAR) for a 15-h photoperiod. Wound treatment was applied to the stem of the previous year's growth with wounds made every 5 mm along two opposite sides of the stem. The full length of the stem was mechanically wounded in April 2009 by making horizontal cuts with a razor blade. Samples (xylem and bark) were collected along the wounded section of stem at 1, 2, 4 and 8 days after wounding. Xylem and bark of four replicates for each time point were collected. Stem sections were cut and xylem and bark were separated manually and immediately frozen independently in liquid nitrogen and stored at -80oC until RNA extraction. A normalized, full-length enriched, directionally cloned library was prepared by Evrogen (Moscow, Russia) with the Creator SMART cDNA Library Construction Kit (Clontech, Mountain View, CA, USA) and the TRIMMER-DIRECT cDNA Normalization Kit (Evrogen). Total RNA was individually extracted from each bark tissue sample using the large scale RNA extraction method described in Chang, S., et al. (1993) Plant Mol. Biol. Reporter 11(2):113-116 and DNase I treated (New England Biolabs). Total RNA from one replicate for each time point was pooled and 17 micrograms of total RNA was sent to Evrogen. First strand cDNA was prepared from 300 nanograms of total RNA, PowerScript Reverse Transcriptase (Clontech), CDS-3M primer (Evrogen), and the SMART IV Oligonucleotide (Clontech). Second strand cDNA was prepared by LD-PCR with Advantage 2 Polymerize mix (Clontech). This cDNA was then normalized with duplex-specific nuclease and amplified following the TRIMMER-DIRECT protocol, digested with SfiI, size fractionated, and cDNA larger than approx. 1000 bp was ligated into SfiI-digested pDNR-LIB (Clontech). The ligation mixture was transformed into ElectroMAX DH10B T1 Phage-Resistant electro-competent cells (Invitrogen). Titre of the primary library (1 mL) was approx. 6.3x10^5 cfu/mL and was not amplified.

text: E. coli DH10B

text: Pinus banksiana

text: Sfi I

text: Sfi I

text: Previous year's bark (phloem and cambium attached) from mechanically wounded trees

text: Pba01-Bark-wounded-Norm (PBA01)

text: Plasmid

text: pDNR-LIB


text: University of British Columbia

text: live

text: Generic

text: Jack pine seedlings were provided by Mike Bendzsak (Forest First, SK) in January 2009. Seed originated from the 'Prince Albert West' seedlot in western Saskatchewan, and was initially grown at the PRT Armstrong Nursery field site. The seedlings were one year old when received at the University of Alberta. Seedlings were transplanted to 3.78 L plastic pots filled with Sunshine Mix #4 (Sun Gro Horticulture Canada TM) growing media. The plants were watered every two to three days in a growth chamber under the following conditions: 19oC (day/night) temperature, relative humidity of 20-35% and under full spectrum fluorescent lamps (200 micromol PAR) for a 15-h photoperiod. Wound treatment was applied to the stem of the previous year's growth with wounds made every 5 mm along two opposite sides of the stem. The full length of the stem was mechanically wounded in April 2009 by making horizontal cuts with a razor blade. Samples (xylem and bark) were collected along the wounded section of stem at 1, 2, 4 and 8 days after wounding. Xylem and bark of four replicates for each time point were collected. Stem sections were cut and xylem and bark were separated manually and immediately frozen independently in liquid nitrogen and stored at -80oC until RNA extraction. A normalized, full-length enriched, directionally cloned library was prepared by Evrogen (Moscow, Russia) with the Creator SMART cDNA Library Construction Kit (Clontech, Mountain View, CA, USA) and the TRIMMER-DIRECT cDNA Normalization Kit (Evrogen). Total RNA was individually extracted from each xylem tissue sample using the large scale RNA extraction method described in Chang, S., et al. (1993) Plant Mol. Biol. Reporter 11(2):113-116 and DNase I treated (New England Biolabs). Total RNA from one replicate for each time point was pooled and 20 micrograms of total RNA was sent to Evrogen. First strand cDNA was prepared from 300 nanograms of total RNA, PowerScript Reverse Transcriptase (Clontech), CDS-3M primer (Evrogen), and the SMART IV Oligonucleotide (Clontech). Second strand cDNA was prepared by LD-PCR with Advantage 2 Polymerize mix (Clontech). This cDNA was then normalized with duplex-specific nuclease and amplified following the TRIMMER-DIRECT protocol, digested with SfiI, size fractionated, and cDNA larger than approx. 1000 bp was ligated into SfiI-digested pDNR-LIB (Clontech). The ligation mixture was transformed into ElectroMAX DH10B T1 Phage-Resistant electro-competent cells (Invitrogen). Titre of the primary library (1 mL) was approx. 1.0x10^6 cfu/mL and was not amplified.

text: E. coli DH10B

text: Pinus banksiana

text: Sfi I

text: Sfi I

text: Previous year's xylem from mechanically wounded trees

text: Pba02-Xylem-wounded-Norm (PBA02)

text: Plasmid

text: pDNR-LIB


text: University of Maryland

text: live

text: Generic

text: SMART cDNA library construction kit (Clontech) following the manufacturer's protocol

text: whole body

characteristic: organ

text: Drosophila pseudoobscura

text: Mather17, Mather48, Abajo36, AFC2

characteristic: strain

text: Drosophila pseudoobscura life stages cDNA library


characteristic: External Id

text: National Museum of Marine Biology and Aquarium

text: live

text: Generic

characteristic: NCBI submission package

text: Stylophora pistillata

text: Stylophora esposed to Aroclor 1254 library

text: y&T vextor


text: Prince of Songkla University

text: live

text: Generic

text: Haemolymph

characteristic: cell type

text: Adult

text: E.coli TG1

text: Haemolymph

text: Penaeus monodon

characteristic: organism

text: Sfi I

text: Not I

text: Male and female

text: Haemolymph

text: Shrimp adult haemolymph

text: Phage


text: National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency

characteristic: INSDC center name

text: live

text: Generic

text: The forward subtractive library of cDNA in stages I (previtellogenic) and III (early cortical rod) ovaries of the giant tiger shrimp (Penaeus monodon) broodstock

characteristic: description

text: E. coli JM109

text: Ovaries

text: Penaeus monodon

text: Female

text: Shrimp Ovary SSH Forward Library

text: Plasmid

text: pGEM-T Easy


text: live

text: Generic

text: The reverse subtractive library of cDNA in stages I (previtellogenic) and II (vitelogenic) ovaries of the giant tiger shrimp (Penaeus monodon) broodstock

text: E. coli JM109

text: Ovaries

text: Penaeus monodon

text: Female

text: Shrimp Ovary SSH Reverse Library

characteristic: title

text: Plasmid

text: pGEM-T Easy

characteristic: vector


text: Beijing Academy of Agriculture and Forestry Science

characteristic: INSDC first public

text: live

text: Generic

text: The three-week old leaves were infected by Peronospora parasitica. Amplifying primers were designed according to the already-known Brassica rapa defense-related genes.

text: three weeks

characteristic: dev-stage

text: Brassica rapa

text: hermaphrodite

characteristic: sex

text: leaves

text: Peronospora parasitica induced defense-related genes in Brassica rapa


text: USDA-ARS Western Regional Research Center

characteristic: INSDC last update

text: live

text: Generic

text: The cDNA library was made from poly(A) selected mRNA isolated from cold acclimated bark tissue harvested from Parthenium argentatum AZ-2. Poly(A) mRNA was converted to double-stranded cDNA by using a modified oligo(dT) primer (aactggaagaattcgcggccgcaccgatttttttttttttttttvn). The double-stranded cDNA was ligated to an EcoRI adaptor (aattccgttgcgtcg) and the adaptored cDNA was size selected for fragments > 600 bps. The cDNAs were then digested with NotI and EcoRI and ligated into the NotI/EcoRI sites of pBluescript II SK+. The resulting cDNA library contained ~1.3 x 106 clones. Quality Read Lengths from sequences were extracted with Phred program. File trimming based on sequence mark-up done with cross_match program, minor vector/adaptor sequences remained.

text: cold acclimated

text: Parthenium argentatum

text: EcoRI

text: NotI

text: bark

characteristic: tissue-type

text: Parthenium argentatum cold acclimated bark cDNA library (PAB)

text: Plasmid

text: pBluescript II SK+


text: live

characteristic: INSDC status

text: Generic

text: cold acclimated

text: Parthenium argentatum

text: EcoRI

text: NotI

text: bark

text: Parthenium argentatum cold acclimated bark cDNA library (PBC)

text: Plasmid

text: pBluescript II SK+


text: Washington and Lee University

text: live

text: Generic

characteristic: NCBI submission model

text: Total RNA was extracted from 3 whole adult female spiders. Library construction was performed by BIO S&T (Montreal, Canada). SMART cDNA library construction kit (Clontech, USA) was used. cDNA was normalized and subjected to SfiI digestion and size fractionation. Fragments greater than 0.5kb were purified and ligated into pBluescript II SK(-) with SfiI A&B inserts between EcoRI and XhoI.

text: Gravid adult females

text: Latrodectus hesperus

text: female

text: wild population, Riverside, CA

text: whole spiders

text: Latrodectus hesperus normalized cDNA library

text: phagemid

text: pBluescript II SK(-)


text: United Planters' Association of Southern India-Tea Research Foundation, Tea Research Institute

text: live

text: Generic

characteristic: cultivar

text: Creator SMART cDNA library construction kit (Clonetech) was used. mRNA from uninfected and Pestalotiopsis theae infected leaves were used as driver and tester respectively

text: Camellia sinensis

text: Leaf

text: Forward subtractive library from uninfected and Pestalotiopsis theae infected leaves of Camellia sinensis

text: Plasmid


text: live

text: Generic

text: Creator SMART cDNA library construction kit (Clonetech) was used. mRNA from uninfected and Pestalotiopsis theae infected leaves were used as tester and driver respectively

text: Camellia sinensis

text: Leaf

text: Reverse subtractive library from uninfected and Pestalotiopsis theae infected leaves of Camellia sinensis

text: Plasmid


text: RIKEN, Plant Science Center

text: live

text: Generic

text: Striga hermonthica

text: mixture of seedlings treated with strigol, or rice root exudates, leaves, flowers and roots

text: full-length enriched Striga hermonthica cDNA library


text: National Institute of Agrobiological Sciences

text: live

text: Generic

text: dehydrated after 36hr

text: larva 4-days

text: Polypedilum vanderplanki

text: mixed

text: Nigerian

characteristic: strain

text: whole body

text: pBluescript SK-


characteristic: External Id

text: live

text: Generic

text: dehydrated after 12hr

text: larva 4-days

text: Polypedilum vanderplanki

text: mixed

text: Nigerian

text: whole body

text: pBluescript SK-


text: live

text: Generic

characteristic: NCBI submission package

text: dehydrated after 0hr

text: larva 4-days

text: Polypedilum vanderplanki

text: mixed

text: Nigerian

text: whole body

text: pBluescript SK-


text: Kazusa DNA Research Institute;

text: live

text: Generic

text: ecotype Gifu B-129 or Miyakojima MG-20 ; The full-length cDNA library was constructed by the Cap-Trapper technology. Total RNAs from A) shoots of Miyakojima MG-20, B) roots of Miyakojima MG-20, C) suspension-cultured cells of Gifu B-129, and D) suspension-cultured cells of Gifu B-129 treated with 0.1% yeast extract were mixed and used for construction of the cDNA library. A nucleotide tag A) TACG, B) CATG, C) CTGA or D) GTCA, respectively, was attached at the end of the polyA sequence to distinguish the source of the mRNA.

text: Lotus japonicus


text: Kazusa DNA Research Institute;

text: live

text: Generic

text: ecotype Gifu B-129 ; Equal amounts of total RNAs prepared from Lotus japonicus B-129 suspension-cultured cells treated with A) 0.5 mM salicylic acid for 6 hr and B) 0.5 mM methyljasmonate for 24 hr were mixed and used for construction of the cDNA library.

text: Lotus japonicus


text: Kyoto University

text: live

text: Generic

text: Acacia mangium

text: mixture of developing secondary xylem and shoot

text: Acacia mangium developing secondary xylem and shoot normalized cDNA library


text: College of Horticulture Northwest A&F University

text: live

text: Generic

text: phage cDNA library was constructed by the Clontech SMART cDNA Library Construction Kit, then the phage library was converted by the recombinase-mediated recombination of lTriplEx2 cycled at loxP sites to pTriplEx2 of bacteria library.

text: young leaves between 3~4 node from shoot apex

text: E.coli XL1-Blue

characteristic: lab-host

text: leaves

characteristic: organ

text: Vitis pseudoreticulata

text: Cycled cDNA library of young leaves of Chinese wild Vitis inoculated with Uncinula necator

text: plasmid


text: Alfred-Wegener-Institut fur Polar- und Meeresforschung

characteristic: INSDC center name

text: live

text: Generic

text: Including ESTs from different physiological conditions: non-stressed, dark shock, cold shock, high salinity, low salinity, N-limitation, P-limitation, 7 timepoints during photocycle

text: Alexandrium minutum

characteristic: organism

text: Alexandrium minutum normalized EST library


text: University of Northern Colorado

text: live

text: Generic

text: Differential expression PCR conducted with GeneFishing (SeeGene); cDNA was subcloned using TOPO-TA (pCR4.0) vector.

characteristic: description

text: Hymenaea courbaril

text: Leaf

text: Hymenaea heat-stress response

characteristic: title

text: TOPO-TA (pCR4.0)


text: University of Northern Colorado

characteristic: INSDC first public

text: live

text: Generic

text: Hymenaea courbaril

text: Leaf

text: Hymenaea MeJA response

text: TOPO-TA (pCR4.0)

characteristic: vector


text: Gene Research Center, University of Tsukuba1; Institute of Sustainable Halophyte Utilization (ISHU), University of Karachi2

text: live

text: Generic

text: Seeds of Aeluropus lagopoides (Linn.) Trin. Ex Thw. (stoloniferous, perennial grass) were collected from natural habitats and grown with sand culture in plastic pots at a constant temperature of 25oC and 12:12 h Light:Dark photoperiod regime. Each pot contained only one individual which was sub-irrigated with 1/2 standard Hoagland solution made with tap water. The nutrient solution was replaced on weekly basis. Similar sized 2 month old plants were selected for salinity treatment of 400 mM NaCl which was achieved by gradual increments of 100 mM every alternate day. After 48 h of reaching the target salinity level, leaves were selected for total RNA extraction using RNAqueous kit and Plant RNA isolation aid solution (Ambion). The first strand cDNA synthesis was carried out by using RNA PCR kit (AMV) (Takara). RT-PCR was performed with the above mentioned pair of primers, which were designed on the basis of conserved regions of targeted gene from other plants in the literature. Amplified product was clone and sequence through E. coli (DH5a), pGEM-T easy vector system II and TA cloning kit (Invitrogen).

text: Mature (from node # 2 and 3)

characteristic: dev-stage

text: E. coli (DH5a)

text: Aeluropus lagopoides

text: Leaves

text: High-salt (400 mM NaCl) treated Aeluropus lagopoides leaves library

text: plasmid

text: pGEM-T


text: Northwest Sci-Tech University of Agriculture and Forestry

characteristic: INSDC last update

text: live

text: Generic

text: The mRNA from the skin of Andrias davidianus was purified and the first-strand cDNA was synthesized by reverse transcription with SMART Oligonucleotide and CDS 3' PCR Primer 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-d(T)30N-1N-3'(N =A,G,C,or T; N-1=A,G, or C). The SMART Oligonucleotide and the CDS 3'PCR Primer contained Sfi IA and Sfi IB restriction enzyme sites respectively. The first-strand cDNA was used as a template to amplify the double-strand cDNA by long-distance PCR (LD-PCR) with 5' PCR primer (5'- CGCCAGGGTTTTCCCAGTCACGAC -3') and the CDS 3' PCR Primer.The product was purified and digested by Sfi I (IA and IB)restriction enzyme. The purified double-strand cDNA was ligated into the Sfi I-digested transformated pUC19 vector and the recombinant DNA was packaged in vitro. The phages were transducted to bacteria Escherichia coli DH10B and then were amplified.

text: DH10B E.coli

text: skin

text: Andrias davidianus

text: Sfi IA

text: Sfi IB

text: skin

text: cDNA Library from the skin of Andrias davidianus


text: Kunming University of Science and Technology

text: live

text: Generic

text: exposed to 2mM formaldehyde for 0.5,2,4,and 24 hours respectively

text: leaf

text: Hedera helix

text: leaf

characteristic: tissue-type

text: Leaf Supression Substractive Hybridization cDNA Library of ivy

text: pGEM-T Easy Vector


text: Anhui Agricultural University

text: live

characteristic: INSDC status

text: Generic

text: YJ63X and YJ63Y

characteristic: cell-line

text: Camellia sinensis

text: hermaphrodite

characteristic: sex

text: callus

text: cDNA-AFLP of tea callues with different catechins contents

text: pMD18-T Simple


text: Horticultural Institute, Zhejiang Academy of Agricultural Sciences

text: live

text: Generic

characteristic: NCBI submission model

text: Tiancao

characteristic: cultivar

text: cDNA sequences of differentially expressed TDF isolated from the 'Tiancao' tangor (Citrus sinensis x Citrus reticulata cv. Tiancao) under drought stress condition by cDNA-AFLP technique.

text: Citrus sinensis x Citrus reticulata

text: cDNA fragments of drought-stress-induced genes from 'Tiancao' tangor


characteristic: External Id

text: live

text: Generic

characteristic: NCBI submission package

text: treated with 2mM formaldehyde for 0.5,2,4,and 24 hours respectively

text: leaf

text: Rohdea japonica

text: leaf

text: Supression Substractive Hybridization cDNA Library of Rohdea japonica under formaldehyde stress

text: pGEM-T Easy


text: National Institute of Allergy and Infectious Diseases

text: live

text: Generic

text: salivary gland

characteristic: cell type

text: PCR-based cDNA library made with SMART cDNA library construction kit from Clontech

text: adult

text: salivary gland

characteristic: organ

text: Phlebotomus sergenti

text: female

text: Turkey

characteristic: strain

text: salivary gland

text: PSSG

text: Lambda TriplEx2


text: University of Helsinki

characteristic: INSDC center name

text: live

text: Generic

text: country: Finland:Baltic Sea

characteristic: description

text: Furcellaria lumbricalis

characteristic: organism

text: Furcellaria lumbricalis Baltic Sea


text: University of Helsinki

characteristic: INSDC first public

text: live

text: Generic

text: country: United Kingdom:Northern Ireland, Atlantic Ocean

text: Furcellaria lumbricalis

text: Furcellaria lumbricalis Northern Ireland, Atlantic Ocean

characteristic: title


text: Instituto Gulbenkian de Ciencia

characteristic: INSDC last update

text: live

text: Generic

text: Leptopilina boulardi

text: female

text: Leptopilina boulardi female


text: live

text: Generic

text: salivary gland

text: adult

characteristic: dev-stage

text: salivary gland

text: Phlebotomus tobbi

text: female

text: Turkey

text: salivary gland

characteristic: tissue-type

text: PTbSG

text: Lambda TriplEx2

characteristic: vector


text: Wuhan University

text: live

characteristic: INSDC status

text: Generic

text: Adult

text: Heterometrus petersii

text: Venom gland

text: Heterometrus petersii venom gland Library


text: South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences

text: live

text: Generic

characteristic: NCBI submission model

text: Creator SMART cDNA Construction Kit (Clonetech) for library construction and TRIMMER-DIRECT kit (Evrogen) for normalization.

text: Mature with shell length 40 +/- 5mm

characteristic: lab-host

text: Whole Body

text: Haliotis diversicolor

text: SfiI

text: SfiI

text: Female, Male

characteristic: sex

text: Normalized Bacteria Challenged Haliotis diversicolor Reeve whole body library

text: Plasmid

text: pDNR-LIB


characteristic: External Id

text: Agricultural Research Service

text: live

text: Generic

characteristic: NCBI submission package

text: Library was constructed using long-distance PCR (SMART cDNA Library method) and the pCR4-TOPO plasmid vector

text: Mixed sex adults, 1-7 days post-eclosion, fed and unfed

text: antennae and maxillary palps

text: Stomoxys calcitrans

text: female and male

text: KBUSLIRL laboratory strain

text: Adult stable fly antennae-maxillary palp cDNA library

text: Plasmid

text: pCR4 TOPO


text: live

text: Generic

text: PCR-based cDNA library made with SMART cDNA Library Construction kit from Clontech

text: adult, blood fed

text: midgut

characteristic: organ

text: Phlebotomus perniciosus

text: female

text: Spain

text: midgut

text: PPRGF

text: Lambda TriplEx 2


text: live

text: Generic

text: adult, sugar fed

text: midgut

text: Phlebotomus perniciosus

text: female

text: Spain

characteristic: strain

text: midgut

text: PPRGU

text: Lambda TriplEx 2


text: Ghent University

characteristic: INSDC center name

text: live

text: Generic

text: Suppression subtractive hybridization (tester: in vivo derived equine blastocysts; driver; in vitro produced equine blastocysts

text: Blastocyst

text: Equus caballus

text: SSH equine blastocysts


text: National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University

text: live

text: Generic

text: Waterlogging induced SSH library

characteristic: description

text: root

text: Zea mays

text: Waterlogging induced SSH library


text: Institute of Aquaculture Torre de la Sal (CSIC)

text: live

text: Generic

text: Sparus aurata

characteristic: organism

text: IATS Aquamax gilthead sea bream SSH selection

text: PCR 2.1


text: INRA

characteristic: INSDC first public

text: live

text: Generic

text: A laboratory strain of S. littoralis was permanently reared on a semi-artificial diet at 23oC, 60-70percent relative humidity, under a 16:8 light:dark cycle and sexed as pupae A normalization step was introduced in the library construction that consisted of a DNA target-RNA driver hybridization and substraction procedure (Invitrogen).

text: Adult

text: antennae

text: Spodoptera littoralis

text: male

text: antennae

text: Spodoptera littoralis male antennae Library

text: Plasmid


text: USDA-ARS Western Regional Research Center

text: live

text: Generic

text: The cDNA library was made from poly(A) selected mRNA isolated from stem tissue of Ficus elastica. Quality Read Lengths from sequences were extracted with Phred program. File trimming based on sequence mark-up done with cross_match program, minor vector/adaptor sequences remained.

text: Ficus elastica

text: stem

text: Ficus elastica stem cDNA library (FES)

characteristic: title

text: Plasmid


text: National Research Institute of Aquaculture

characteristic: INSDC last update

text: live

text: Generic

text: Subtractive hybridization was performed with the PCR-Selectct DNA Subtraction Kit (Clontech), according to the manufacture's protocol. Total RNA from peripheral blood leucocyte of Edwardsiella tarda injected fish and phosphate buffered saline (PBS) injected fish was used to make tester and driver cDNAs, respectively. Two subtractions were performed: the original intended subtraction (forward subtraction), and a reverse subtraction in which tester serves as the driver and the driver as tester.

text: adult

text: Paralichthys olivaceus

text: mixed

text: peripheral blood leucocyte


text: live

text: Generic

text: adult

characteristic: dev-stage

text: Paralichthys olivaceus

text: mixed

text: peripheral blood leucocyte

text: peripheral blood leucocyte of PBS injected fish


text: College of Urban Sciences, University of Seoul

text: live

text: Generic

text: Chironomus riparius differentially expressed gene upon exposure to silver nanoparticles (AgNPs)

text: Fourth instar larva

text: Chironomus riparius

text: C.riparius silver nanoparticles treated


text: Kunming University of Science and Technology

text: live

text: Generic

text: leaf

text: Cymbidium grandiflorum

text: leaf

characteristic: tissue-type

text: Percicarp Smart cDNA Library of Cymbidium grandifiorium

text: pGEM-T Easy


text: Konkuk University

text: live

text: Generic

text: The libraries were constructed by using SMART kit (Clontech, Cat No. 634901). lambda Triplex 2 vector was used for cloning of double stranded cDNA from spleen and neocortex libraries. Electro-competent DH10b cells were used for transformation.

text: Sus scrofa

text: Korean native pig

text: Full-length enriched cDNA from Korean native pig

text: lambda Triplex 2

characteristic: vector


text: Nanjing Agricultural University

text: live

characteristic: INSDC status

text: Generic

text: Summer Black

characteristic: cultivar

text: Vitis vinifera

text: Fruits and flower

text: Grape cv. Summer Black flower and fruit


text: University of Bologna

text: live

text: Generic

characteristic: NCBI submission model

text: HcrVf2 transgenic Gala line Ga2-2

text: Young expanding leaves of the scab-resistant Ga2-2 transgenic line, challenged with V. inaequalis, were collected at three time points (0, 24 and 48 hours post-inoculation). Total RNA was extracted by the phenol-SDS method and treated with DNase (Amersham). RNA was reverse transcribed into a double-stranded cDNA using an oligodT 25V primer. Ds-cDNA was digested with EcoRI and MseI, ligated to adaptors and amplified with different combinations of +3/+3 selective primers. cDNA-AFLP fragments were separated by liquid chromatography using the dHPLC WAVE System (Transgenomic) and selected peaks were collected using the FCW200 fragment collector. Eluted fractions were sequenced with the Big Dye 3.1 chemistry and AB 3730 sequencer following the manufacturer's protocol.

text: Malus domestica

text: young leaves

text: TDFs from V. inaequalis challenged GM apple leaves


text: Washington University School of Medicine

text: live

text: Generic

text: cDNA was prepared from 2 ug of poly A+ RNA (equal parts from stage 10.5 and stage 11.5 gastrulae). EcoRI-XhoI cut cDNA was then ligated into UniZap-XR (Stratagene) with EcoRI at the 5' end and XhoI at the 3' end. The library was mass excised and used to infect Top10F'. Clones were picked into freezing medium (per liter 15 g tryptone, 10g yeast extract, 5g NaCl, 36 mM K2HPO4, 13.2 mM KH2PO4, 1.7 mM Na-citrate, 0.4 mM MgSO4 7 H2O, 6.8 mM (NH4)2SO4, 4 % w/v glycerol) and grown for 24 hours. Original library construction by Bruce Blumberg (Cho et al 1991 Cell 67, 1111-1120).

text: Top-10 F'

characteristic: lab-host

text: Xenopus laevis

text: EcoRI

text: XhoI

text: gastrula (stages 10.5, 11.5 mixed)

text: Xenopus laevis gastrula non normalized

text: phagemid (ampicillin resistant)

text: pBluescript SK-


characteristic: External Id

text: live

text: Generic

text: cDNA was prepared from 2 ug of poly A+ RNA. EcoRI-XhoI cut cDNA was then ligated into UniZap-XR (Stratagene) with EcoRI at the 5' end and XhoI at the 3' end. The library was mass excised and used to infect Top10F'. Clones were picked into freezing medium (per liter 15 g tryptone, 10g yeast extract, 5g NaCl, 36 mM K2HPO4, 13.2 mM KH2PO4, 1.7 mM Na-citrate, 0.4 mM MgSO4 7 H2O, 6.8 mM (NH4)2SO4, 4 % w/v glycerol) and grown for 24 hours. Original library construction by Bruce Blumberg (Blumberg et al., 1991 Science 253, 194-196; Hawlet et al., 1995, Genes Dev. 9, 2923-2935).

text: Top-10 F'

text: Xenopus laevis

text: EcoRI

text: XhoI

text: oocyte (stages 5 and 6)

text: Xenopus laevis oocyte non normalized

text: phagemid (ampicillin resistant)

text: pBluescript SK-


text: Department of Botany & Plant Sciences, University of California

text: live

text: Generic

characteristic: NCBI submission package

text: Ruby

text: Two trees with open-pollinated flowers in a mixed planting at UC Riverside Agricultural Operations were the source of tissue. Flower buds were collected by Federici (Roose lab) from January-March 2003 in four groups: 1) no white petals, 2) white petals visible, bud less than 0.5 cm, 3) bud more than 0.5 cm but not open, 4) newly opened flowers. Tissues were snap frozen and then stored at -80C until further processing. Fenton (Close lab) purified RNA by the phenol method described in J. Japanese Soc. Hort. Sci. 1996. 64 (4): 809-814, purified poly(A) mRNA using a PolyATtrack mRNA Isolation System IV (Promega), produced a primary cDNA library using a lambda ZAP XR cDNA Synthesis Kit (Stratagene), then mass-excised one million pfu from the primary library to produce a phagemid population. Phagemids were plated, plasmid DNA purified, cDNA clones archived, and DNA sequences determined bi-directionally using an ABI3730 at the Arizona Genomics Institute, University of Arizona (Kim, Kudrna, Stum, Yost, Wing). Chromatogram files were downloaded by FTP to UC Riverside (by Close), then processed at UC Riverside (by Wanamaker, Close lab) using the HarvEST pipeline (http://harvest.ucr.edu) to remove vector and cloning oligo sequences and various contaminants, and to trim to a high quality region. Sequences that retained a phred 17 region of at least 100 bases were deposited to GenBank.

text: 20 year old trees

text: E. coli TJC121

text: Citrus sinensis

text: EcoRI

text: XhoI

text: Flower

text: Ruby Orange Developing Flower cDNA Library

text: Phagemid pBluescript SK(-)

text: Lambda Uni-ZAP XR, excised phagemid


text: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences

text: live

text: Generic

text: Sporulated oocysts were obtained from feces of E. maxima infected chickens. Sporulated oocysts subtractive PCRs were performed using the PCR-select cDNA subtractive kit according to the manufacture's protocol (Clontech). PCR products then were cloned into the pGEM-T Easy vector (Promega) and transformed into JM109.

text: sporulated oocysts

text: Eimeria maxima

text: sporulated oocysts subtracted cDNA library

text: pGEM-T-easy Vector(Promega)


text: MPI for Molecular Genetics

text: live

text: Generic

text: Total RNA was extracted from shock-frozen tissue. Library construction was performed with Cloneminer (Invitrogen) starting from enriched mRNA.

text: embryo

text: Rattus norvegicus

text: BN/SsNHsd/Mcwi

text: etcmemb


text: Tsinghua University

text: live

text: Generic

text: on 7th, 14th and 21th day regeneration; intact flatworms; flatworms under stress

text: Dugesia japonica

text: asexual

characteristic: sex

text: whole body

text: Dugesia japonica Pek-1 strain Library


text: INRA - UMR BIOGECO

text: live

text: Generic

text: differentiating xylem collected along the growing season

text: Pinus pinaster

text: differentiating xylem


text: INRA - UMR BIOGECO

text: live

text: Generic

text: white roots collected on 4 pedonculate oaks

text: Quercus robur

text: white roots

text: WZOAQRAR


text: INRA - UMR BIOGECO

text: live

text: Generic

text: sessile oaks

text: Quercus petraea

text: swelling buds

text: WZOAQPAB


text: INRA - UMR BIOGECO

text: live

text: Generic

text: pedonculate oaks

text: Quercus robur

text: swelling buds

text: WZOAQRAQ


text: INRA - UMR BIOGECO

text: live

text: Generic

text: sessile oaks

text: Quercus petraea

text: young leaves

text: WZOAQPAC


text: INRA - UMR BIOGECO

text: live

text: Generic

text: sessile oaks

text: Quercus petraea

text: secondary differentiating xylem

text: WZOAQPAG


text: INRA - UMR BIOGECO

text: live

text: Generic

text: adult pedonculate oaks

text: Quercus robur

text: setting buds

text: WZOAQRAP


text: INRA - UMR BIOGECO

text: live

text: Generic

text: one year old cuttings

text: Quercus petraea

text: white roots

text: WZOAQPAE WZOAQPAF


text: INRA - UMR BIOGECO

text: live

text: Generic

text: sessile oaks

text: Quercus petraea

text: roots

text: WZOAQPAA


text: INRA - UMR BIOGECO

text: live

text: Generic

text: one year old cuttings

text: Quercus robur

text: green leaves

text: WZOAQRAM


text: INRA - UMR BIOGECO

text: live

text: Generic

text: one year old cuttings

text: Quercus robur

text: white roots

text: WZOAQRAI


text: INRA - UMR BIOGECO

text: live

text: Generic

text: one year old cuttings

text: Quercus robur

text: white roots

text: WZOAQRAK


text: INRA - UMR BIOGECO

text: live

text: Generic

text: one year old cuttings

text: Quercus petraea

text: white roots

text: WZOAQPAD


text: Institute of Marine Research

text: live

text: Generic

text: Gadus morhua

text: Norwegian coastal cod

characteristic: strain

text: Head kidney

text: CHO


text: Institute of Marine Research

characteristic: INSDC center name

text: live

text: Generic

text: Gadus morhua

text: Norwegian coastal cod

text: Liver

text: CLE


text: Institute of Marine Research

text: live

text: Generic

text: Gadus morhua

text: Norwegian coastal cod

text: Pituitary

text: CHY


text: Institute of Marine Research

text: live

text: Generic

text: Immature

text: Gadus morhua

text: Norwegian coastal cod

text: Liver

text: CLU


text: Institute of Marine Research

text: live

text: Generic

text: 3-6 days post fertilization

text: Gadus morhua

text: Norwegian coastal cod

text: Embryo

text: CEG


text: Institute of Marine Research

text: live

text: Generic

text: Gadus morhua

text: Norwegian coastal cod

text: Pylerus

text: CPY


text: Institute of Marine Research

text: live

text: Generic

text: 9 days post fertilizization

text: Gadus morhua

text: Norwegian coastal cod

text: Embryo

text: CEM


text: Institute of Marine Research

text: live

text: Generic

text: Gadus morhua

text: Norwegian coastal cod

text: Testis

text: CTE


text: Institute of Marine Research

text: live

text: Generic

text: Gadus morhua

text: Norwegian coastal cod

text: Brain

text: CHJ


text: Institute of Marine Research

text: live

text: Generic

text: Gadus morhua

text: Norwegian coastal cod

text: Ovary

text: COV


text: Institute of Marine Research

text: live

text: Generic

text: Gadus morhua

text: Norwegian coastal cod

text: Spleen

text: CMI


text: Institute of Marine Research

text: live

text: Generic

text: Gadus morhua

text: Norwegian coastal cod

text: Spleen, immunologically stimulated

text: CSMI


text: Institute of Marine Research

text: live

text: Generic

text: Gadus morhua

text: Norwegian coastal cod

text: Intestine

text: CTA


text: Institute of Marine Research

text: live

text: Generic

text: Gadus morhua

text: Norwegian coastal cod

text: Beard

text: CGG


text: Institute of Marine Research

text: live

text: Generic

text: Immature

text: Gadus morhua

text: Norwegian coastal cod

text: Brain

text: CHU


text: Institute of Marine Research

text: live

text: Generic

text: Gadus morhua

text: Norwegian coastal cod

text: Head kidney, immunologically stimulated

text: CSH


text: Institute of Marine Research

text: live

text: Generic

text: Gadus morhua

text: Norwegian coastal cod

text: Thymus

text: CTH


text: Institute of Marine Research

text: live

text: Generic

text: Gadus morhua

text: Norwegian coastal cod

text: Eye

text: COY


text: Institute of Marine Research

text: live

text: Generic

text: Gadus morhua

text: Norwegian coastal cod

text: Skin

text: CNN


text: Institute of Marine Research

text: live

text: Generic

text: Pre-feeding

text: Gadus morhua

text: Norwegian coastal cod

text: Larva

text: CLF


text: Assam Agricultural University

text: live

text: Generic

text: According to the instruction given in Clontech Manual on SMART cDNA Library Construction Kit Manual (No. 634901; Clonetech Lab Inc, USA)

characteristic: description

text: Camellia sinensis

text: Leaf

text: pTriplx


text: Second Military Medical University

text: live

text: Generic

text: adult females at 24 h post feeding on a Plasmodium yoelii infected blood meal

text: adult

text: whole body

characteristic: organ

text: Anopheles sinensis

characteristic: organism

text: female

text: whole body

text: Anopheles sinensis infected with Plasmodium yoelii


text: National Dairy Research Institute

characteristic: INSDC first public

text: live

text: Generic

text: Oocyte

characteristic: cell type

text: Subtracted cDNA library was prepared from oocytes as a tester vs Different somatic tissues like Heart, Kidney, Lung, Intestine, Spleen, Liver, cumulus as a Driver.

text: Oocyte were collected at 0,8,16,24 hours of maturation

text: XL-Blue

text: Bubalus bubalis

text: Female

text: Ovary

text: Subtracted cDNA library from Buffalo oocyte

characteristic: title

text: pGEMT


text: Consiglio Nazionale delle Ricerche

characteristic: INSDC last update

text: live

text: Generic

text: anamorph: Pochonia chlamydosporia var. chlamydosporia. cDNA-AFLP technique. Pochonia chlamydosporia var. chlamydosporia mycelium was collected at 0, 2, 4 and 8 h after treatments (nutrient-rich medium, predicted to repress parasitism; minimal medium, predicted to de-repress genes associated with parasitism; and minimal medium with addition of nematode eggs). RNA was extracted and cDNA was synthesized using oligo dT. The cDNA was digested with MseI and BstYI. Adapters were ligated to the termini, and two rounds of PCR were performed. The second one considering two selective additional bases at the 3' end.

text: Pc10 from Root Knot Nematode

characteristic: isolate

text: Pochonia chlamydosporia

text: mycelium culture

characteristic: tissue-type


text: Consiglio Nazionale delle Ricerche

text: live

characteristic: INSDC status

text: Generic

text: Pc280 from Potato Cyst Nematode

text: Pochonia chlamydosporia

text: mycelium culture


text: Nanjing Agricultural University

text: live

text: Generic

characteristic: NCBI submission model

text: Xiyeqing

characteristic: cultivar

text: Fruits and flowers of Prunus mume 'Xiyeqing' at different development stages were collected from the resource nursery of Nanjing Agricultural University, Najing PRC. The materials were collected from 2009.2-2009.5. The flowers included four growing stages: small bud, medium bud, half-unfolding flower and completely unfolding flower, while the fruits were collected at 0.3, 0.6, 0.9, 1.2, 1.8, 2.4, 3.6 cm diameter stages.

text: mixed

characteristic: dev-stage

text: Escherichia coli DH10B

text: Fruits and flowers

text: Prunus mume

text: Fruits and flowers

text: Fruits and flowers of Prunus mume cv. Xiyeqing

text: pDNR-LIB

characteristic: vector


characteristic: External Id

text: Bioforsk Plantehelse

text: live

text: Generic

characteristic: NCBI submission package

text: diploid strawberry variety FDP821. Phytophthora cactorum-strawberry interaction library was constructed using suppression subtractive hybridization (SSH) technique in which the infected strawberry crowns at 1, 3 and 5 days post-inoculation were pooled to be the tester and the comparative control crowns collected at the separate designated time points were pooled to be the driver.

text: Phytophthora cactorum

text: Bioforsk isolate ID 10300


text: live

text: Generic

text: Hevea brasiliensis

text: Hevea brasiliensis resistance SSH cDNA sequence induced by BTH


text: VTT Technical Research Centre of Finland

characteristic: INSDC center name

text: live

text: Generic

text: Trichoderma reesei

characteristic: strain

text: Hypocrea jecorina Rut-C30


text: University of Nevada Las Vegas

text: live

text: Generic

text: cells grown in 3oC seawater

characteristic: description

text: Chlamydomonas sp. CCMP681

text: Chlamydomonas CCMP681 3oC normalized cDNA


text: Bioforsk Plantehelse

text: live

text: Generic

text: Phytophthora cactorum germinating cyst library was constructed using suppression subtractive hybridization (SSH) technique in which germinating cysts were used as the tester and sporangia were used as the driver

text: cyst germination

text: Bioforsk isolate ID 10300

text: Phytophthora cactorum

characteristic: organism


text: Bioforsk Plantehelse

characteristic: INSDC first public

text: live

text: Generic

text: A differential display-based screening technique was set up to specifically identify and isolate from Phytophthora cactorum RxLR-EER effector-encoding genes differentially expressed during cyst germination and strawberry infection.

text: Bioforsk isolate ID 10300

text: Phytophthora cactorum

text: Phytophthora cactorum differential display library


text: SRM University

text: live

text: Generic

text: Seed specific full length enriched normalized cDNA library was constructed from developing seeds of Jatropha curcas L. The size of the developing seeds ranged between 0.5 cm and 2.5 cm.

text: Jatropha curcas

text: EcoRI

text: XhoI

text: Developing seeds

text: Seed specific Normalized cDNA library from Jatropha curcas L.

text: Plasmid

text: pBluescript II SK-


text: Institute of plant Biology, Wroclaw University

text: live

text: Generic

text: PCR products using specific primers with cDNA as a template.

text: seedlings

text: roots

characteristic: organ

text: Cucumis sativus

text: hermaphrodite

characteristic: sex

text: Cucumis sativus seedling roots


text: British Antarctic Survey

characteristic: INSDC last update

text: live

text: Generic

text: directional cDNA from L. elliptica gill tissue under control and heat stress conditions

text: Laternula elliptica

text: gill

text: Laternula elliptica heat stress gill tissue

characteristic: title

text: plasmid

text: pDNR-LIB


text: British Antarctic Survey

text: live

text: Generic

text: directional cDNA from L. elliptica mantle tissue under control and heat stress conditions

text: Laternula elliptica

text: mantle

characteristic: tissue-type

text: Laternula elliptica heat stress mantle tissue

text: plasmid

text: pDNR-LIB


text: British Antarctic Survey

text: live

characteristic: INSDC status

text: Generic

text: directional cDNA from L. elliptica siphon tissue under control and heat stress conditions

text: Laternula elliptica

text: siphon

text: Laternula elliptica heat stress siphon tissue

text: plasmid

text: pDNR-LIB


text: National Institute of Nutrition and Seafood Research

text: live

text: Generic

characteristic: NCBI submission model

text: SSH library constructed from pooled liver samples of adult Atlantic herring (Clupea harangues) dietary exposed to Calanus finmarchicus enriched with crude oil.

text: Adult

text: Clupea harengus

text: Male

text: Liver

text: Atlantic herring dietary exposed to crude oil

text: pGEM T-Easy


text: Institute of Agriculture, Lithuanian Research Centre for Agriculture and Forestry

text: live

text: Generic

text: Zolis

characteristic: cultivar

text: Timothy plants were drought-stressed for 6 days and cDNA-AFLP profiled against unstressed plants

text: day 6 of drought stress

text: leaf

text: Phleum pratense

text: Timothy drought stress Library


text: Universidad de San Martin

text: live

text: Generic

text: trypomastigote

characteristic: cell type

text: TcT-E: Epimastigote-subtracted Trypomastigote cDNA library. Total RNA was isolated from trypomastigotes and epimastigotes of T. cruzi with TriZOL (Gibco-BRL). mRNA was purified with the polyA-Tract mRNA isolation system (Promega). The PCR-Select cDNA Subtraction kit (Clontech) was used for library construction following the selective subtractive hybridization protocol. First strand cDNA synthesis was initiated with 2 ug of polyA+ of each T. cruzi stage (trypomastigote and epimastigote), an oligo dT primer containing a 5' RsaI site and Superscript II reverse transcriptase (Gibco-BRL). Second strand cDNA synthesis was performed with T4 DNA polymerase. After RsaI digestion of double strand cDNA, two different sets of adaptors (1: CTAATACGACTCACTATAGGGCTCGAGCGGCCGCCCGGGCAGGT; 2R: CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGT) were ligated to the tester cDNA (trypomastigotes) but not to the driver cDNA (epimastigotes). Two rounds of subtractive hybridization in the presence of an excess of driver cDNA were performed, leading to the enrichment of differentially expressed sequences in the tester cDNA population that were the templates for further suppressive PCR amplification performed with adaptor-specific primers (First PCR primer: CTAATACGACTCACTATAGGGC; Nested PCR primer 1: TCGAGCGGCCGCCCGGGCAGGT; Nested PCR primer 2R: AGCGTGGTCGCGGCCGAGGT). The subtraction efficiency was verified by monitoring the PCR amplification of T. cruzi histone 2A transcript in subtracted and unsubtracted samples. PCR products enriched for tester differentially expressed sequences (longer than 100 bp) were cloned into the pGEM T-easy vector (Promega).

text: trypomastigote

characteristic: dev-stage

text: Trypanosoma cruzi

text: RsaI

text: RsaI

text: CL-Brener

text: TcT-E

text: plasmid

text: pGEM-T Easy