text: University of Wisconsin

text: live

text: Generic

text: Nipponbare

text: The library contains RT-PCR products derived from CRR (Centromeric Retrotransposon of Rice). Total RNA was reversely transcribed into cDNA using random priming method. The cDNA was used as a template for PCR amplification using CRR-specific primers (CRR1_2F: 5' CTC CTC TCA TCG AAA GAT CG 3' and CRR1_3R: 5' GGC TGC AAG TAA TAT CAA GGT AC 3'). RTPCR products were resolved on an agarose gel and only shorter, less abundant fragment was cloned.

text: flowering

text: panicle

text: Oryza sativa Japonica Group


status: PUBLIC

characteristic: External Id

text: Universiti Putra Malaysia

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Oryza sativa Indica Group

characteristic: organism

text: EcoRV

tag: attribute

text: Oryza sativa indica, Clontech PCR select cDNA subtraction

characteristic: title

text: Bacterial

text: TOPO-pCR Bunt (Invitrogen)

characteristic: vector


text: Chinese Academy of Sciences

text: live

text: Generic

text: Guang Lu Ai 4

characteristic: cultivar

text: Oryza sativa Indica Group

text: Oryza sativa (indica cultivar-group) cv. Guang Lu Ai 4


text: Beijing Weiming Kaituo Agriculture Biotech Co., Ltd.

text: live

text: Generic

text: E.coli SOLR/E.coli XLOLR

characteristic: lab-host

text: Oryza sativa Japonica Group

text: mixture of leaf, root, panicle

characteristic: tissue-type

text: Rice mixture of leaf, root, panicle

text: pGM-T/pCT-T/pBluscript II SK(-)


text: Institut de Recherche pour le Developpement

text: live

text: Generic

text: Azucena

text: Oryza sativa Japonica Group

text: Oryza sativa (japonica cultivar-group) cv. Azucena cDNA-AFLP fragment


text: live

text: Generic

text: Oryza sativa Indica Group

text: Oryza sativa (indica cultivar-group) cv. IR64 cDNA-AFLP fragment


text: CIRAD

text: live

text: Generic

text: Nona Bokra

text: Oryza sativa Indica Group

text: Salt stress SSH library


text: CIRAD

text: live

text: Generic

text: Nipponbare

text: description

characteristic: description

text: Oryza sativa Japonica Group

text: Osmotic stress SSH library


text: Academia Sinica

text: live

text: Generic

text: Tainung 67

text: cDNA prepared from rice young panicle mRNA by using oligo(dT) as the primer for ligation into the SfiIA-SfiIB sites of phagemid pTripleEx2.

text: 6cm long panicle

characteristic: dev-stage

text: Oryza sativa Japonica Group

text: panicle

text: 6cm rice panicles


text: Jawaharlal Nehru University

text: live

text: Generic

text: Oryza sativa Indica Group

text: Seedling

text: OSE

text: Phage


text: Jawaharlal Nehru University

text: live

text: Generic

text: Pokkali

text: Oryza sativa Indica Group

text: Root

text: OSR

text: Phage


text: Jawaharlal Nehru University

text: live

text: Generic

text: Pokkali

text: Oryza sativa Indica Group

text: Shoot

text: OSS

text: Phage


text: Department of Agriculture, Thailand

text: live

text: Generic

text: A cDNA library was constructed from blast-infected rice leaf with Magnaporthe grisea (Pathumthani strain) at 24 hours post-inoculation.

text: 3 weeks

text: E. coli XL-10 Gold

text: Oryza sativa Indica Group

text: EcoRI

text: XhoI

text: leaf

text: blast-infected rice leaf cDNA library from 3-week-old O. Sativa plant

text: pBluescriptII SK(+) XR (Stratagene)


text: Central Institute Of Brackishwater Aquaculture

text: live

text: Generic

text: adult

text: Penaeus indicus

text: pleopod, gill

text: WSSV infected EST library from Indian white shrimp F.indicus

text: plasmid

text: pSPORT 1


text: live

text: Generic

text: adult

text: Penaeus monodon

text: gill

text: WSSV infected EST library from Indian tiger shrimp P.monodon

text: plasmid

text: pSPORT 1


text: Roslin Institute

text: live

text: Generic

text: DC/Mphage/Mo

characteristic: cell type

text: Ex vivo ALDCs + M. bovis. MoDCs + M. bovis.

text: Bos taurus

text: Eco RI

text: Not I

text: KN510 M. bovis infected Bos taurus DC/Mphage/Mo

text: pBluescriptII(SK+)


text: Roslin Institute

text: live

text: Generic

text: DC/Mphage/Mo

text: Ex vivo ALDCs. uninfected MoDCs.

text: Bos taurus

text: Eco RI

text: Not I

text: KN510 Bos taurus uninfected DC/Mphage/Mo

text: pBluescriptII(SK+)


text: Roslin Institute

text: live

text: Generic

text: lymphocytes

text: PBMC + PPD-B 5 day culture. PBMC + PPD-B 3 day culture. Lymph Node RNA.

text: Bos taurus

text: Eco RI

text: Not I

text: lymph nodes

text: KN510 Bos taurus lymphocytes/lymph nodes

text: pBluescriptII(SK+)


text: Roslin Institute

text: live

text: Generic

text: Bovine RAJ challenged with 026. Bovine RAJ challenged with Walla. Bovine RAJ from animals which failed to colonise. Bovine terminal rectum from treated animals. Bovine rectal mucosa from treated animals.

text: Bos taurus

text: Eco RI

text: Not I

text: rectum

text: KN510 Bos taurus rectum from E. coli challenged/treated animals

text: pBluescriptII(SK+)


text: Roslin Institute

text: live

text: Generic

text: Bovine RAJ from unchallenged animals

text: Bos taurus

text: Eco RI

text: Not I

text: rectum

text: KN510 Bos taurus rectum from E. coli unchallenged animals

text: pBluescriptII(SK+)


text: Roslin Institute

text: live

text: Generic

text: Bovine colon challenged with 026. Bovine spiral colon challenged with 026. Bovine proximal colon patch from infected/treated animals - trimmed of follicular material. Bovine peyer's patch from infected/treated animals - trimmed of follicular material. Bovine non-peyer's patch terminal ileum.

text: Bos taurus

text: Eco RI

text: Not I

text: colon/ileum

text: KN510 Bos taurus colon/ileum tissue from E. coli challenged/treated/uninfected animals

text: pBluescriptII(SK+)


text: Roslin Institute

text: live

text: Generic

text: sequence provenance lost

text: Bos taurus

text: Eco RI

text: Not I

text: pBluescriptII(SK+)


text: Roslin Institute

text: live

text: Generic

text: 2 samples from day 21 after primary infection. 2 samples from 2 month trickle infection. 2 samples from challenge infection.

text: Ovis aries

text: Eco RI

text: Not I

text: abomasal mucosa

text: KN511 Ovis aries abomasal mucosa (gastric fold) from Teladorsagia infected sheep

text: pBluescriptII(SK+)


text: Roslin Institute

text: live

text: Generic

text: samples from 2 worm free sheep.

text: Ovis aries

text: Eco RI

text: Not I

text: abomasal mucosa

text: KN511 Ovis aries abomasal mucosa (gastric fold) from (Teladorsagia) uninfected sheep

text: pBluescriptII(SK+)


text: Roslin Institute

text: live

text: Generic

text: 2 samples from day 21 after primary infection. 2 samples from 2 month trickle infection, not challenged. 2 samples from challenge infection.

text: Ovis aries

text: Eco RI

text: Not I

text: gastric lymph node

text: KN511 Ovis aries gastric lymph node from Teladorsagia infected sheep

text: pBluescriptII(SK+)


text: Roslin Institute

text: live

text: Generic

text: 2 timepoints from 2 sheep undergoing primary infection. 2 timepoints from 2 sheep undergoing challenge infection, plus prechallenge lymph from each sheep.

text: Ovis aries

text: Eco RI

text: Not I

text: efferent gastric lymph

text: KN511 Ovis aries efferent gastric lymph from Teladorsagia infected sheep

text: pBluescriptII(SK+)


text: Roslin Institute

text: live

text: Generic

text: samples from 2 sheep before primary Teladorsagia infection.

text: Ovis aries

text: Eco RI

text: Not I

text: efferent gastric lymph

text: KN511 Ovis aries efferent gastric lymph from uninfected sheep

text: pBluescriptII(SK+)


text: Roslin Institute

text: live

text: Generic

text: 2 samples from 2 month trickle infection. 2 samples from challenge infection.

text: Ovis aries

text: Eco RI

text: Not I

text: peyer's patch from terminal ileum

text: KN511 Ovis aries peyer's patch from terminal ileum of Teladorsagia infected sheep

text: pBluescriptII(SK+)


text: Roslin Institute

text: live

text: Generic

text: Ovine rectum challenged with NCTC12900 day 1. Ovine rectum challenged with NCTC12900 day 2. Ovine rectum unchallenged. Ovine large colon challenged with NCTC12900 x2. Ovine large colon challenged with 026 k60. Ovine large colon unchallenged.

text: Ovis aries

text: Eco RI

text: Not I

text: rectum / large colon

text: KN511 Ovis aries rectum and large colon from E. coli challenged and unchallenged animals

text: pBluescriptII(SK+)


text: Roslin Institute

text: live

text: Generic

text: sequence provenance lost.

text: Ovis aries

text: Eco RI

text: Not I

text: pBluescriptII(SK+)


text: Genoscope - Centre National de Sequencage

text: live

text: Generic

text: Clytia hemisphaerica

text: Clytia hemisphaerica library


text: DOE Joint Genome Institite

text: live

text: Generic

text: The library was prepared from total RNA using the Creator SMART cDNA Library Construction Kit with the LD-PCR method to amplify the cDNA. Amplified cDNA was digested with SfiI, size selected for >400bp, and ligated into the pDNR-LIB vector.

text: 13 day old larvae

text: ElectroMAX DH10B

text: Orbicella faveolata

text: SfiI

text: SfiI

text: CAON Montastraea faveolata 13 day old larvae

text: pDNR-LIB


text: DOE Joint Genome Institite

text: live

text: Generic

text: The library was prepared from total RNA using the Creator SMART cDNA Library Construction Kit with the LD-PCR method to amplify the cDNA. Amplified cDNA was digested with SfiI, size selected for >400bp, and ligated into the pDNR-LIB -lib vector. WARNING: this library contains a small percentage of cDNAs derived from the coral's symbiont, Symbiodinium sp.

text: ElectroMAX DH10B

text: Orbicella faveolata

text: SfiI

text: SfiI

text: CAOO Montastraea faveolata 13 day old larvae infected with Symbiodinium

text: pDNR-LIB


text: DOE Joint Genome Institite

text: live

text: Generic

text: 13 day old larvae

text: ElectroMAX DH10B

text: Orbicella faveolata

text: SfiI

text: SfiI

text: CAGI Montastraea faveolata 13 day old larvae

text: pDNR-LIB


text: DOE Joint Genome Institite

text: live

text: Generic

text: ElectroMAX DH10B

text: Orbicella faveolata

text: SfiI

text: SfiI

text: CAGP Montastraea faveolata 13 day old larvae infected with Symbiodinium

text: pDNR-LIB


text: Central Tuber Crops Research Institute

text: live

text: Generic

text: Mycelial

text: After 7 days of growth of P. colocasiae in defined liquid Media (Edward et al., 1987)

text: Colocasia esculenta

text: Phytophthora colocasiae

text: 98-111 (Sikkim, Assam, India)

characteristic: strain

text: Mycelium

text: Elicitor gene of Phytophthora colocasiae

text: pGEM-T


characteristic: External Id

text: American Museum of Natural History

text: live

text: Generic

text: Around 200 male Drosophila mayaguana flies (stock 15081-1397.03 from the Tucson Stock Center) were dissected for their accessory glands. Dissections were done in PBS with RNA later (Ambion). Accessory glands were pooled together and kept in RNA later at -20oC until RNA extraction. Total RNA was extracted with the RNAeasy extraction kit (QIAGEN) and mRNA was isolated from total RNA using the Oligotex mRNA kit (QIAGEN). cDNA synthesis was done with the Super SMART cDNA Synthesis Kit (Clontech). Female cDNA generated by the same process was used as probe for subtraction of housekeeping genes and other non-male specific genes using the PCR-Select cDNA Subtraction Kit (Clontech). The last PCR product of the subtraction process was size fractionated for fragments larger than ~700bp. Size fractionation was carried out in 2% low-melting agarose gel stained with SYBR. PCR fragments were retrieved from the gel using the Wizard gel purification kit (PROMEGA). After a quick incubation with Taq polymerase for adding overhanging A's, the retrieved fragments were cloned into a TA plasmid vector (Topo TA cloning kit, Invitrogen). Chemically competent cells (Invitrogen) were then transformed to produce an accessory gland subtracted library.

text: adults

text: E. coli

text: accessory gland

characteristic: organ

text: Drosophila mayaguana

text: male

characteristic: sex

text: Tucson Stock Center 15081-1397.03

text: Accessory gland cDNA library

text: plasmid


text: UMR INRA/Universite Henri Poincare 1136

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Mycelium of Lacaria bicolor S238N was grown 21 days on cellophane-covered agar containing modified Pachlewski medium (pH 5.5). This consisted of (mM) 12.5 (NH4)2tartrate, 110 D-glucose, 14 maltose, 7 KH2PO4, 2 MgSO4; microelements (gl-1) 6.00 iron, 0.27 molybdenum, 8.45 boron, 5.00 manganese, 0.625 copper, 2.27 zinc, Kanieltra 6Fe, and thiamine-HCl, 100 mg l-1. Colonies were then transferred on complete Pachlewski liquid medium for two days acclimatising period and then N, P or C starved during a time course experiment of 6 days. Control mycelium was grown in complete Pachlewski liquid medium. Fruiting bodies of L. bicolor S238N were collected below Douglas fir seedlings grown in a nursery and inoculated using L. bicolor S238N. Tissues were immediately frozen in liquid nitrogen and RNA extractions were carried out using the RNeasy Plant Mini Kit (Qiagen). Total RNA from different tissues was pooled (18% derived from fruiting bodies) and mRNA was purified using Oligotex mRNA spin columns (Qiagen). cDNA was synthesized using the CLONTECH SMART cDNA library synthesis Kit(Ozyme). First-strand cDNA synthesis was performed as described in the provided protocol using 150 ng mRNA. Double-stranded cDNA was prepared from 2ul of the first strand reaction by PCR (25 cycles) with the provided primers in a 100ul reaction and cDNA was purified using a QIAquick PCR purification column (Qiagen). Adapter ligation, nebulization and DNA sequencing was performed by COGENICS (France) according to their protocol and using a 70x75mm region of a genome sequencer GS-FLX run (Roche Diagnostics).

text: free-living mycelium and fruiting bodies

text: Laccaria bicolor

text: Free-living mycelium and fruiting bodies from Laccaria bicolor S238N


text: National Fisheries Research & Development Institute

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Haliotis discus hannai

text: Haliotis discus hannai cold-shock subtracted cDNA library


text: live

text: Generic

text: Haliotis discus hannai

characteristic: organism

text: Haliotis discus hannai heat-shock subtracted cDNA library

characteristic: title


text: live

text: Generic

text: Haliotis discus hannai

text: Haliotis discus hannai hyposalinity subtracted cDNA library


text: live

text: Generic

text: Paralichthys olivaceus

text: bowel

text: Paralichthys olivaceus bowel cDNA library


text: live

text: Generic

text: Paralichthys olivaceus

text: gill

text: Paralichthys olivaceus gill cDNA library


text: live

text: Generic

text: Paralichthys olivaceus

text: stomach

text: Paralichthys olivaceus stomach cDNA library


text: National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency

text: live

text: Generic

text: Conventional cDNA library of testes of shrimp broodstock

text: E. coli XL-1 Blue MRF

characteristic: lab-host

text: Testes

text: Penaeus monodon

text: Male

text: Shrimp Testis cDNA Library

text: phage

text: UNIZAP XR

characteristic: vector


text: Medical University of South Carolina

text: live

text: Generic

text: Libraries were prepared using the SMART cDNA Library Construction Kit (Clontech)

characteristic: description

text: Penaeus vannamei

text: Sfi I

text: Sfi I

text: eyestalk

characteristic: tissue-type

text: Litopenaeus vannamei eyestalk cDNA library

text: plasmid


text: Medical University of South Carolina

text: live

text: Generic

text: Penaeus vannamei

text: Sfi I

text: Sfi I

text: gills

text: Litopenaeus vannamei gills cDNA library

text: plasmid


text: Medical University of South Carolina

text: live

text: Generic

text: Penaeus vannamei

text: Sfi I

text: Sfi I

text: hemocyte

text: Litopenaeus vannamei hemocyte cDNA library

text: plasmid


text: Medical University of South Carolina

text: live

text: Generic

text: Penaeus vannamei

text: Sfi I

text: Sfi I

text: hepatopancreas

text: Litopenaeus vannamei hepatopancreas cDNA library

text: plasmid


text: Medical University of South Carolina

text: live

text: Generic

text: Penaeus vannamei

text: Sfi I

text: Sfi I

text: lymphoid organ

text: Litopenaeus vannamei lymphoid organ cDNA library

text: plasmid


text: Medical University of South Carolina

text: live

text: Generic

text: Penaeus vannamei

text: Sfi I

text: Sfi I

text: nerve cord

text: Litopenaeus vannamei nerve cord cDNA library

text: plasmid


text: College of Horticulture, Northwest A&F University

text: live

text: Generic

characteristic: cultivar

text: mRNA differential display was employed to study anthracnose resistant gene express differential in the wild grape (Vitis L.) native to China. cDNA fragments relate to anthracnose resistant gene were obtained.

text: Vitis quinquangularis

text: Female

text: Leaves

text: Vitis quinquangularis Shang-24 leaves


text: Adam Mickiewicz University

text: live

text: Generic

text: ventus

text: seedlings grown in the dark for 3 days at 25C

characteristic: dev-stage

text: Lupinus luteus

text: hypocotyl

text: 72h polyA RNA


characteristic: External Id

text: Chengdu Biology Institute of Chinese Academy of Science

text: live

text: Generic

text: root of wheat inoculated with cereal cyst nematodes

text: Triticum aestivum

text: root

text: wheat influenced by cereal cyst nematode different display Library


text: South China Agricultural University

text: live

text: Generic

text: The cDNAs were isolated from leaves of cold-treated cold-tolerant mutant of Stylosanthes guianensis

text: Stylosanthes guianensis

text: Stylosanthes guianensis SSH Library of cold-responsive cDNA


text: Fujian Agriculture and Forestry University

text: live

text: Generic

text: Capsicum annuum

text: Capsicum annuum (under stress) cDNA-AFLP


text: National Institute for Plant Genome Research

text: live

text: Generic

text: Xanthi

text: seedling

text: Nicotiana tabacum

text: subtracted cDNA library of CAP2 expressing N. tabacum


text: Tohoku University

text: live

text: Generic

text: immature anther

text: Oryza sativa Japonica Group

text: ms-bo-Taichung 65

characteristic: strain

text: anther

text: Oryza sativa ms-bo-Taichung 65 immature anther


text: Sichuan University

text: live

text: Generic

text: sperm cell

characteristic: cell type

text: cDNA was prepared from isolated rice sperm cells and amplified by PCR with the help of SMART cDNA Library Construction Kit(Clontech). Directional cDNAs were inserted into lambda TriplEx2 vector at 5'end with SfiIA and 3' end with SfiIB site.

text: isolated from mature pollen of rice

text: E.coli XL1-Blue

text: Oryza sativa Indica Group

text: SfiI A

text: SfiI B

text: Rice sperm cells lambda TriplEx2 cDNA library

text: phage

text: lambda TriplEx2


text: Research Association for Biotechnology

characteristic: INSDC center name

text: live

text: Generic

text: Akitakomachi

text: country=Japan

text: Oryza sativa Japonica Group

text: leaf

text: Oryza sativa cv. Akitakomachi leaf


text: University of Wisconsin-Madison

characteristic: INSDC first public

text: live

text: Generic

text: Yuanfengzao (a susceptible variety to rice blast disease)

text: Rice seedlings were pretreated by foliar spraying with 0.3 mM benzothiadiazole (BTH) and then inoculated with Magnaporthe grisea three days after BTH treatment. Leaf samples were collected twenty-four hour after inoculation and mRNAs were used for construction of the differentially expressed cDNA libraires by suppression subtractive hybridization.

text: Three-week-old rice seedlings pretreated with BTH and/or inoculated with Magnaporthe grisea

text: Oryza sativa Indica Group

text: Seedling leaves

text: Differentially expressed cDNA libraries of BTH-treated/blast fungus-infected rice leaf tissues

text: pGEM-T Easy


text: University of Hyderabad

characteristic: INSDC last update

text: live

text: Generic

text: Nagina 22 (indica sub sp)

text: ESTs from normalized root cDNA Library from drought stressed seedlings

text: 35 day-old seedlings

text: Leaf

characteristic: organ

text: Oryza sativa Indica Group

text: Entire root tissue

text: Drought stress (root)

text: Phagemid


text: University of Delhi South Campus

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Oryza sativa Indica Group

text: Oryza sativa cv. CSR27 PCR-based subtractive cloning


text: University of Delhi South Campus

text: live

text: Generic

characteristic: NCBI submission package

text: Pokalli

text: Oryza sativa Indica Group

characteristic: organism

text: Oryza sativa cv. Pokalli PCR-based subtractive cloning


text: Kansas State University

text: live

text: Generic

text: Xa7/avrXa7 interaction library clones: From reverse subtracted cDNA libraries of rice near-isogenic line BB7 carrying the Xa7 gene for resistance and inoculated with Xanthomonas oryzae pv. oryzae either with (resistant interaction) or without (susceptible interaction) the avrXa7 gene. Partial cDNA sequence

text: 14-15 day seedling

text: E.coli.

characteristic: lab-host

text: Oryza sativa Indica Group

text: Leaf

text: Rice Xa7/avrXa7 interaction reverse subtractive cDNA library

characteristic: title

text: TOPO cloning vector


text: Kansas State University

text: live

text: Generic

text: Xa10/avrXa10 interaction library clones: From reverse subtracted cDNA libraries of rice near-isogenic line BB7 carrying the Xa10 gene for resistance and inoculated with Xanthomonas oryzae pv. oryzae either with (resistant interaction) or without (susceptible interaction) the avrXa10 gene. Partial cDNA sequence

characteristic: description

text: 14-15 day seedling

text: E.coli.

text: Oryza sativa Indica Group

text: Leaf

text: Rice Xa10/avrXa10 interaction reverse subtractive cDNA library

text: TOPO cloning vector

characteristic: vector


text: Kansas State University

text: live

text: Generic

characteristic: cultivar

text: Xa7/avrXa7 interaction library clones: From forward subtracted cDNA libraries of rice near-isogenic line BB7 carrying the Xa7 gene for resistance and inoculated with Xanthomonas oryzae pv. oryzae with (resistant interaction)the avrXa7 gene. Partial cDNA sequence.

text: 14-15 day seedling

text: E.coli.

text: Oryza sativa Indica Group

text: Leaf

characteristic: tissue-type

text: Rice Xa7/avrXa7 interaction forward subtractive cDNA library

text: TOPO cloning vector


characteristic: External Id

text: Kansas State University

text: live

text: Generic

text: Xa10/avrXa10 interaction library clones: From forward subtracted cDNA libraries of rice near-isogenic line BB7 carrying the Xa10 gene for resistance and inoculated with Xanthomonas oryzae pv. oryzae either with (resistant interaction) or without (susceptible interaction) the avrXa10 gene. Partial cDNA sequence

text: 14-15 day seedling

characteristic: dev-stage

text: E.coli.

text: Oryza sativa Indica Group

text: Leaf

text: Rice Xa10/avrXa10 interaction forward subtractive cDNA library

text: TOPO cloning vector


text: University of Illinois

text: live

text: Generic

text: Drought stressed and controls.

text: ElectroMax DH10B

text: Oryza sativa Indica Group

text: Leaf

text: Uni-ZAP XR


text: Korea University

text: live

text: Generic

text: Donganbyeo

text: The rice mutant resistant to growth inhibition by 5MT were selected from the callus irradiated with gamma ray of 50 Gy through rice cv. Donganbyeo embryo culture.For construction of the cDNA library, 5MT resistant homologous rice mutants were treated by 5MT for 3 weeks.

text: seedling grown for 3 weeks

text: XL1-Blue MRF'

text: Oryza sativa Japonica Group

text: 5MT treated leaves, stems, and roots

text: 5MT resistant rice mutant cDNA library

text: pBluescripts SK(+/-) phagemid


text: Genomics and Genetics Institute, GreenGene Biotech Inc.; Division of Bioscience and Bioinformatics, MyongJi University

text: live

text: Generic

text: Nackdong

text: mRNA was capped with oligoribonucleotides and then used as templates for RT-PCR.

text: 14 days after germination

text: E.coli DH10B

text: Oryza sativa Japonica Group

text: EcoRI

text: leaf

text: Rice etiolated leaf plasmid cDNA library (14ETL)

text: Plasmid


text: live

text: Generic

text: Nackdong

text: 14 days after germination

text: E.coli DH10B

text: Oryza sativa Japonica Group

text: EcoRI

text: root

text: Rice root plasmid cDNA library (14ROOT)

text: Plasmid


text: live

text: Generic

text: Nackdong

text: 30 days after germination

text: E.coli DH10B

text: Oryza sativa Japonica Group

text: EcoRI

text: leaf

text: Rice leaf plasmid cDNA library I (30DGS)

text: Plasmid


text: live

text: Generic

text: Nackdong

text: 7 days after germination

text: E.coli DH10B

text: Oryza sativa Japonica Group

text: EcoRI

text: leaf

text: Rice leaf plasmid cDNA library II (7LEAF)

text: Plasmid


text: live

text: Generic

text: Nackdong

text: Leaf was dried for 2hrs. cDNA was inserted into lamda Uni-ZAP XR vector at 5' end with EcoRI and 3' end with XhoI site. mRNA was prepared from ABA-responsive element binding transcription factor 3 overexpression line.

text: 14 days after germination

text: E.coli SOLR

text: Oryza sativa Japonica Group

text: EcoRI

text: XhoI

text: leaf

text: ABF3-overexpressing transgenic rice lambda phage cDNA library (ABF1)

text: phage

text: pBluescript SK(+)


text: live

text: Generic

text: Nackdong

text: Leaf was incubated at 4 C(360uM/m-2sec-1) for 2hrs. Oligo-capped mRNA was reverse transcribed and then used for PCR.

text: 14 days after germination

text: E.coli DH10B

text: Oryza sativa Japonica Group

text: EcoRI

text: leaf

text: Rice cold treated leaf plasmid cDNA library (CLD1)

text: Plasmid


text: live

text: Generic

text: Nackdong

text: Callus was treated with ABA(20um) for 1hour. cDNA was inserted into lambda Uni-ZAP XR vector at 5' end with EcoRI and 3' end with XhoI site. mRNA was derived from rice Histone Deacetylase overexpression line.

text: proliferated callus on 2N6 media for 2 weeks

text: E.coli SOLR

text: Oryza sativa Japonica Group

text: EcoRI

text: XhoI

text: callus

text: OsHDAC1-overexpressing transgenic rice lambda phage cDNA library I (HDA1)

text: phage

text: pBluescript SK(+)


text: live

text: Generic

text: Nackdong

text: Leaf was dried for 2hrs. Oligo-capped mRNA was reverse transcribed and then used for PCR. mRNA was prepared from ABA-responsive element binding transcription factor 3 overexpression line.

text: 14 days after germination

text: E.coli DH10B

text: Oryza sativa Japonica Group

text: EcoRI

text: leaf

text: ABF3-overexpressing transgenic rice plasmid cDNA library (ABF)

text: Plasmid


text: live

text: Generic

text: Nackdong

text: Callus was treated with ABA(20um) for 1hr. Oligo-capped mRNA was reverse transcribed and then used for PCR. mRNA was derived from rice Histone Deacetylase overexpression line.

text: E.coli DH10B

text: Oryza sativa Japonica Group

text: EcoRI

text: callus

text: OsHDAC1-overexpressing transgenic rice plasmid cDNA library (HD)

text: Plasmid


text: live

text: Generic

text: Nackdong

text: cDNA was inserted into lambda Uni-ZAP XR vector at 5' end with EcoRI and 3' end with XhoI site. mRNA was derived from rice Histone Deacetylase overexpression line.

text: E.coli SOLR

text: Oryza sativa Japonica Group

text: EcoRI

text: XhoI

text: callus

text: OsHDAC1-overexpressing transgenic rice lambda phage cDNA library II (HDN)

text: phage

text: pBluescript SK(+)


text: Genomics and Genetics Institute, GreenGene Biotech Inc.; Division of Bioscience and Bioinformatics, MyongJi University

text: live

text: Generic

text: Nackdong

text: cDNA was inserted into lamda Uni-ZAP XR vector at 5' end with EcoRI and 3' end with XhoI site. mRNA was prepared from Arabidopsis Jasmoinate Carboxyl methyltransferase overexpression line.

text: 14 days after germination

text: E.coli SOLR

text: Oryza sativa Japonica Group

text: EcoRI

text: XhoI

text: leaf

text: AtJMT-overexpressing transgenic rice lambda phage cDNA library (JMT1)

text: phage

text: pBluescript SK(+)


text: live

text: Generic

text: Nackdong

text: mRNA was capped with oligoribonucleotides and then used as templates for RT-PCR.

text: proliferated callus on 2N6 media for 30 days

text: E.coli DH10B

text: Oryza sativa Japonica Group

text: EcoRI

text: callus

text: Rice callus plasmid cDNA library (NACL)

text: Plasmid


text: live

text: Generic

text: Nackdong

text: Oligo-capped mRNA was reverse transcribed and then used for PCR. mRNA was pepared from Arabidopsis Jasmoinate Carboxyl methyltransferase overexpression line.

text: 14 days after germination

text: E.coli DH10B

text: Oryza sativa Japonica Group

text: EcoRI

text: leaf

text: AtJMT-overexpressing transgenic rice plasmid cDNA library (JMT)

text: Plasmid


text: live

text: Generic

text: Nackdong

text: cDNA was inserted into lamda Uni-ZAP XR vector at 5' end with SstI and 3' end with XhoI site. Callus was induced on 2N6 media for 30 days and cultured for 36hrs on regenerated media

text: E.coli SOLR

text: Oryza sativa Japonica Group

text: SstI

text: XhoI

text: callus

text: Regenerated callus lambda phage cDNA library (RCL1)

text: phage

text: pBluescript SK(+)


text: Hangzhou Genomics Institute

text: live

text: Generic

text: trefoil

text: Oryza sativa Indica Group

text: leaf

text: Oryza sativa cv. PA64s leaf cDNA library


text: Hangzhou Genomics Institute

text: live

text: Generic

text: trefoil

text: Oryza sativa Indica Group

text: whole plant

text: Oryza sativa cv. LYP9 trefoil whole plant cDNA library


text: Hangzhou Genomics Institute

text: live

text: Generic

text: tillering

text: Oryza sativa Indica Group

text: whole plant

text: Oryza sativa cv. LYP9 tillering whole plant cDNA library


text: Hangzhou Genomics Institute

text: live

text: Generic

text: sterile

characteristic: cell type

text: heading/flowering

text: Oryza sativa Indica Group

text: panicle

text: Oryza sativa cv. PA64s panicle sterile cDNA library


text: Hangzhou Genomics Institute

characteristic: INSDC center name

text: live

text: Generic

text: ferile

text: heading/flowering

text: Oryza sativa Indica Group

text: panicle

text: Oryza sativa cv. PA64s panicle ferile cDNA library


text: Hangzhou Genomics Institute

characteristic: INSDC first public

text: live

text: Generic

text: booting

text: Oryza sativa Indica Group

text: whole plant

text: Oryza sativa cv. LYP9 booting whole plant cDNA library


text: Chengdu Biology Institute of Chinese Academy of Science

characteristic: INSDC last update

text: live

text: Generic

text: root of wheat inoculated with cereal cyst nematodes

text: Triticum aestivum

text: root

text: wheat influenced by cereal cyst nematode differential express Library


text: Fujian Agriculture and Forestry University

text: live

text: Generic

text: Capsicum annuum

text: Capsicum annuum cv. L11 (under stress) cDNA-AFLP


text: Wuhan University

text: live

text: Generic

text: Adult

text: Lychas mucronatus

text: Venom gland

text: Lychas mucronatus venom gland Library


text: Institute of Genetics and Developmental Biology, Chinese Academy of Sciences

text: live

text: Generic

text: Constructed using the pCMV-Script XR cDNA Library Construction Kit (Stratagene). polyA+ RNA was primered by Xho I-site tagged OligodT, and dCTP-Methylated cDNAs were synthesized. The cDNAs were linked to an EcoRI anchor and digested with Xho I. cDNAs larger than 0.9kb was recovered, ligated to the EcoRI and XhoI restricted plasmid pCMV-Script, and transformed to E. coli XL10-gold.

text: adult

text: E. coli XL10-gold

text: Dissostichus mawsoni

text: brain

text: Antarctic fish Dissostichus mawsoni adult brain library

text: pCMV-Script


text: live

text: Generic

text: adult

text: E. coli XL10-gold

text: Dissostichus mawsoni

text: headkidney

text: Antarctic fish Dissostichus mawsoni adult headkidney library

text: pCMV-Script


text: live

text: Generic

text: adult

text: E. coli XL10-gold

text: Dissostichus mawsoni

text: liver

text: Antarctic fish Dissostichus mawsoni adult liver library

text: pCMV-Script


text: live

text: Generic

text: adult

text: E. coli XL10-gold

text: Dissostichus mawsoni

text: ovary

text: Antarctic fish Dissostichus mawsoni adult ovary library

text: pCMV-Script


text: National Institute of Biomedical Innovation

text: live

text: Generic

text: 1st strand cDNA was primed with an oligo(dT) primer [ATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded using specific 5' and 3' primers and amplified by PCR. The PCR cDNA was synthesized product was digested with SfiI and size selection was performed to exclude fragments <1.5kb. The SfiI-digested PCR product was cloned into distinct DraIII sites of pME18S-FL3. XhoI sites just outside the DraIII sites can be used to isolate the cDNA insert. Libraries were constructed by oligo-capping method.

text: 3 years old

text: Macaca fascicularis

text: DraIII (CACTGTGTG)

text: DraIII (CACTGTGTG)

text: male

characteristic: sex

text: bone marrow

text: macaque bone marrow cDNA library QbmA

text: pME18S-FL3 (Acc.No. AB009864)


text: live

characteristic: INSDC status

text: Generic

text: Cap structure of mRNA may be replaced by guanine (G). This library was made using Vector-capping method. See, Kato, S. et al. (2005) Vector-capping: a simple method for preparing a high-quality Full-Length cDNA Library. DNA Res. 12:53-62.

text: 3 years old

text: Macaca fascicularis

text: Swa I

text: Not I

text: male

text: kidney

text: macaque kidney cDNA library QreB


text: live

text: Generic

characteristic: NCBI submission model

text: 3 years old

text: Macaca fascicularis

text: Swa I

text: Not I

text: male

text: kidney

text: macaque kidney cDNA library QreA


text: DOE Joint Genome Institute

text: live

text: Generic

characteristic: NCBI submission package

text: The library was made from oligo(dT)-primed cDNA and cloned into the pMCL200_cDNA vector. PolyA RNA was primed with oligo(dT) primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'-CGGACGCGTGGG) and digested with NotI. cDNA was size selected >2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pMCL200_cDNA vector. Library was created and sequencing done by the DOE Joint Genome Institute (Walnut Creek, CA).

text: amoeba

text: Naegleria gruberi

text: SalI

text: NotI

text: NEG-M

characteristic: strain

text: CAPG Naegleria gruberi amoeba stage


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT)-primed cDNA and cloned into the pMCL200_cDNA vector. PolyA RNA was primed with oligo(dT) primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'-CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 0.6-2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pMCL200_cDNA vector. Library was created and sequencing done by the DOE Joint Genome Institute (Walnut Creek, CA).

text: amoeba

text: Naegleria gruberi

characteristic: organism

text: SalI

text: NotI

text: NEG-M

text: CAPH Naegleria gruberi amoeba stage


text: DOE Joint Genome Institute

text: live

text: Generic

text: flagellate

text: Naegleria gruberi

text: SalI

text: NotI

text: NEG-M

text: CAZN Naegleria gruberi Flagellate Stage (H)

characteristic: title


text: DOE Joint Genome Institute

text: live

text: Generic

characteristic: description

text: flagellate

text: Naegleria gruberi

text: SalI

text: NotI

text: NEG-M

text: CAZO Naegleria gruberi Flagellate Stage (L)


text: live

text: Generic

text: CF-203 cell line treated with 10-6 M juvenile hormone

characteristic: cell-line

text: Choristoneura fumiferana

text: EcorR I

text: Xho I

text: Spruce Budworm Cf-203 cell line library

text: Plasmid


text: live

text: Generic

text: Larva molting from 5th to 6th instar

text: Choristoneura fumiferana

text: EcorR I

text: Xho I

text: Spruce Budworm molting fifth-sixth larval instar library

text: Plasmid

text: pBluescript SK

characteristic: vector


text: South China Agricultural University

text: live

text: Generic

text: Differentially expressed sequences in female adult Ascaris suum. Microarray analysis of expressed sequence tag libraries constructed by suppression subtractive hybridization.

text: Ascaris suum

text: female

text: whole body

characteristic: tissue-type

text: differentially expressed sequences in female adult Ascaris suum


characteristic: External Id

text: South China Agricultural University

text: live

text: Generic

text: Differentially expressed sequences in male adult Ascaris suum. Microarray analysis of expressed sequence tag libraries constructed by suppression subtractive hybridization.

text: Ascaris suum

text: male

text: whole body

text: differentially expressed sequences in male adult Ascaris suum


text: University of Antwerp

text: live

text: Generic

text: Perfluorooctane sulfonate (PFOS) related library.

text: Cyprinus carpio

text: liver

text: Cyprinus carpio PFOS related cDNA library


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: Stressed by starvation. The library was made from oligo(dT)-primed cDNA and cloned into vector pDNR-LIB (Clontech Laboratories, Inc., Mountain View, CA) using the SMART system (BD Biosciences, San Jose, CA). To generate the first strand, polyA RNA was primed with an oligo(dT) primer (5'-ATTCTAGAGGCCGAGGCGGCCGACATG(T)30 VN-3', where V = A, G, or C). The second strand was synthesized by PCR using primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' followed by digestion of the SfiI sites. cDNA was size selected using Sephadex columns provided with the SMART kit. To maximize the ligation efficiency of large transcripts, the largest size fraction was kept separate from the others. Library was created by the Daphnia Genomics Consortium and sequencing was done at DOE Joint Genome Institute (Walnut Creek, CA). Additional information can be found at http://wfleabase.org/.

text: Adult

characteristic: dev-stage

characteristic: lab-host

text: Daphnia pulex

text: Sfi I A

text: Sfi I B

text: Mixed

text: Log50 (arenata)

text: whole animal

text: pDNR-LIB


text: DOE Joint Genome Institute

characteristic: INSDC center name

text: live

text: Generic

text: Juvenile

text: Daphnia pulex

text: Sfi I A

text: Sfi I B

text: Mixed

text: Log50 (arenata)

text: whole animal

text: pDNR-LIB


text: DOE Joint Genome Institute

characteristic: INSDC first public

text: live

text: Generic

text: In Vivo Conditions: Standard laboratory conditions. The library was made from oligo(dT)-primed cDNA and cloned into vector pDNR-LIB (Clontech Laboratories, Inc., Mountain View, CA) using the SMART system (BD Biosciences, San Jose, CA). To generate the first strand, polyA RNA was primed with an oligo(dT) primer (5'-ATTCTAGAGGCCGAGGCGGCCGACATG(T)30 VN-3', where V = A, G, or C). The second strand was synthesized by PCR using primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' followed by digestion of the SfiI sites. cDNA was size selected using Sephadex columns provided with the SMART kit. To maximize the ligation efficiency of large transcripts, the largest size fraction was kept separate from the others. Library was created by the Daphnia Genomics Consortium and sequencing was done at DOE Joint Genome Institute (Walnut Creek, CA). Additional information can be found at http://wfleabase.org/.

text: Juvenile

text: Daphnia pulex

text: Sfi I A

text: Sfi I B

text: Female

text: Log50 (arenata)

text: whole animal

text: pDNR-LIB


text: DOE Joint Genome Institute

characteristic: INSDC last update

text: live

text: Generic

text: Mixed

text: Daphnia pulex

text: Sfi I A

text: Sfi I B

text: Female

text: Log50 (arenata)

text: whole animal

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: Stressed by exposure to fish predator (Lepomis macrochirus). The library was made from oligo(dT)-primed cDNA and cloned into vector pDNR-LIB (Clontech Laboratories, Inc., Mountain View, CA) using the SMART system (BD Biosciences, San Jose, CA). To generate the first strand, polyA RNA was primed with an oligo(dT) primer (5'-ATTCTAGAGGCCGAGGCGGCCGACATG(T)30 VN-3', where V = A, G, or C). The second strand was synthesized by PCR using primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' followed by digestion of the SfiI sites. cDNA was size selected using Sephadex columns provided with the SMART kit. To maximize the ligation efficiency of large transcripts, the largest size fraction was kept separate from the others. Library was created by the Daphnia Genomics Consortium and sequencing was done at DOE Joint Genome Institute (Walnut Creek, CA). Additional information can be found at http://wfleabase.org/.

text: Adult

text: Daphnia pulex

text: Sfi I A

text: Sfi I B

text: Mixed

characteristic: sex

text: Log50 (arenata)

text: whole animal

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

characteristic: INSDC status

text: Generic

text: Juvenile

text: Daphnia pulex

text: Sfi I A

text: Sfi I B

text: Mixed

text: Log50 (arenata)

text: whole animal

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

characteristic: NCBI submission model

text: In Vivo Conditions: Stressed by hypoxia. The library was made from oligo(dT)-primed cDNA and cloned into vector pDNR-LIB (Clontech Laboratories, Inc., Mountain View, CA) using the SMART system (BD Biosciences, San Jose, CA). To generate the first strand, polyA RNA was primed with an oligo(dT) primer (5'-ATTCTAGAGGCCGAGGCGGCCGACATG(T)30 VN-3', where V = A, G, or C). The second strand was synthesized by PCR using primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' followed by digestion of the SfiI sites. cDNA was size selected using Sephadex columns provided with the SMART kit. To maximize the ligation efficiency of large transcripts, the largest size fraction was kept separate from the others. Library was created by the Daphnia Genomics Consortium and sequencing was done at DOE Joint Genome Institute (Walnut Creek, CA). Additional information can be found at http://wfleabase.org/.

text: Adult

text: Daphnia pulex

text: Sfi I A

text: Sfi I B

text: Mixed

text: Log50 (arenata)

text: whole animal

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

characteristic: NCBI submission package

text: Juvenile

text: Daphnia pulex

text: Sfi I A

text: Sfi I B

text: Mixed

text: Log50 (arenata)

text: whole animal

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: Stressed by low dose exposure to UV. The library was made from oligo(dT)-primed cDNA and cloned into vector pDNR-LIB (Clontech Laboratories, Inc., Mountain View, CA) using the SMART system (BD Biosciences, San Jose, CA). To generate the first strand, polyA RNA was primed with an oligo(dT) primer (5'-ATTCTAGAGGCCGAGGCGGCCGACATG(T)30 VN-3', where V = A, G, or C). The second strand was synthesized by PCR using primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' followed by digestion of the SfiI sites. cDNA was size selected using Sephadex columns provided with the SMART kit. To maximize the ligation efficiency of large transcripts, the largest size fraction was kept separate from the others. Library was created by the Daphnia Genomics Consortium and sequencing was done at DOE Joint Genome Institute (Walnut Creek, CA). Additional information can be found at http://wfleabase.org/.

text: Mixed

text: Daphnia pulex

text: Sfi I A

text: Sfi I B

text: Mixed

text: Log50 (arenata)

characteristic: strain

text: whole animal

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: Stressed by high dose exposure to UV. The library was made from oligo(dT)-primed cDNA and cloned into vector pDNR-LIB (Clontech Laboratories, Inc., Mountain View, CA) using the SMART system (BD Biosciences, San Jose, CA). To generate the first strand, polyA RNA was primed with an oligo(dT) primer (5'-ATTCTAGAGGCCGAGGCGGCCGACATG(T)30 VN-3', where V = A, G, or C). The second strand was synthesized by PCR using primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' followed by digestion of the SfiI sites. cDNA was size selected using Sephadex columns provided with the SMART kit. To maximize the ligation efficiency of large transcripts, the largest size fraction was kept separate from the others. Library was created by the Daphnia Genomics Consortium and sequencing was done at DOE Joint Genome Institute (Walnut Creek, CA). Additional information can be found at http://wfleabase.org/.

text: Mixed

text: Daphnia pulex

characteristic: organism

text: Sfi I A

text: Sfi I B

text: Mixed

text: Log50 (arenata)

text: whole animal

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: Stressed by exposure to arsenic (1348 ug/L of As as sodium arsenite). The library was made from oligo(dT)-primed cDNA and cloned into vector pDNR-LIB (Clontech Laboratories, Inc., Mountain View, CA) using the SMART system (BD Biosciences, San Jose, CA). To generate the first strand, polyA RNA was primed with an oligo(dT) primer (5'-ATTCTAGAGGCCGAGGCGGCCGACATG(T)30 VN-3', where V = A, G, or C). The second strand was synthesized by PCR using primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' followed by digestion of the SfiI sites. cDNA was size selected using Sephadex columns provided with the SMART kit. To maximize the ligation efficiency of large transcripts, the largest size fraction was kept separate from the others. Library was created by the Daphnia Genomics Consortium and sequencing was done at DOE Joint Genome Institute (Walnut Creek, CA). Additional information can be found at http://wfleabase.org/.

text: Mixed

text: Daphnia pulex

text: Sfi I A

text: Sfi I B

text: Female

text: Log50 (arenata)

text: whole animal

characteristic: title

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: Stressed by exposure to cadmium (20 ug/L of Cd as cadmium chloride). The library was made from oligo(dT)-primed cDNA and cloned into vector pDNR-LIB (Clontech Laboratories, Inc., Mountain View, CA) using the SMART system (BD Biosciences, San Jose, CA). To generate the first strand, polyA RNA was primed with an oligo(dT) primer (5'-ATTCTAGAGGCCGAGGCGGCCGACATG(T)30 VN-3', where V = A, G, or C). The second strand was synthesized by PCR using primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' followed by digestion of the SfiI sites. cDNA was size selected using Sephadex columns provided with the SMART kit. To maximize the ligation efficiency of large transcripts, the largest size fraction was kept separate from the others. Library was created by the Daphnia Genomics Consortium and sequencing was done at DOE Joint Genome Institute (Walnut Creek, CA). Additional information can be found at http://wfleabase.org/.

characteristic: description

text: Mixed

text: Daphnia pulex

text: Sfi I A

text: Sfi I B

text: Female

text: Log50 (arenata)

text: whole animal

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: Stressed by exposure to zinc (200 ug/L of Zn as zinc chloride). The library was made from oligo(dT)-primed cDNA and cloned into vector pDNR-LIB (Clontech Laboratories, Inc., Mountain View, CA) using the SMART system (BD Biosciences, San Jose, CA). To generate the first strand, polyA RNA was primed with an oligo(dT) primer (5'-ATTCTAGAGGCCGAGGCGGCCGACATG(T)30 VN-3', where V = A, G, or C). The second strand was synthesized by PCR using primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' followed by digestion of the SfiI sites. cDNA was size selected using Sephadex columns provided with the SMART kit. To maximize the ligation efficiency of large transcripts, the largest size fraction was kept separate from the others. Library was created by the Daphnia Genomics Consortium and sequencing was done at DOE Joint Genome Institute (Walnut Creek, CA). Additional information can be found at http://wfleabase.org/.

text: Mixed

text: Daphnia pulex

text: Sfi I A

text: Sfi I B

text: Female

text: Log50 (arenata)

text: whole animal

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: Exposed to Methyl Farnesoate hormone. The library was made from oligo(dT)-primed cDNA and cloned into vector pDNR-LIB (Clontech Laboratories, Inc., Mountain View, CA) using the SMART system (BD Biosciences, San Jose, CA). To generate the first strand, polyA RNA was primed with an oligo(dT) primer (5'-ATTCTAGAGGCCGAGGCGGCCGACATG(T)30 VN-3', where V = A, G, or C). The second strand was synthesized by PCR using primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' followed by digestion of the SfiI sites. cDNA was size selected using Sephadex columns provided with the SMART kit. To maximize the ligation efficiency of large transcripts, the largest size fraction was kept separate from the others. Library was created by the Daphnia Genomics Consortium and sequencing was done at DOE Joint Genome Institute (Walnut Creek, CA). Additional information can be found at http://wfleabase.org/.

text: Adult

text: Daphnia pulex

text: Sfi I A

text: Sfi I B

text: Female

text: Log50 (arenata)

text: whole animal

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: Juvenile

text: Daphnia pulex

text: Sfi I A

text: Sfi I B

text: Female

text: Log50 (arenata)

text: whole animal

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: Stressed by exposure to Microcystis. The library was made from oligo(dT)-primed cDNA and cloned into vector pDNR-LIB (Clontech Laboratories, Inc., Mountain View, CA) using the SMART system (BD Biosciences, San Jose, CA). To generate the first strand, polyA RNA was primed with an oligo(dT) primer (5'-ATTCTAGAGGCCGAGGCGGCCGACATG(T)30 VN-3', where V = A, G, or C). The second strand was synthesized by PCR using primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' followed by digestion of the SfiI sites. cDNA was size selected using Sephadex columns provided with the SMART kit. To maximize the ligation efficiency of large transcripts, the largest size fraction was kept separate from the others. Library was created by the Daphnia Genomics Consortium and sequencing was done at DOE Joint Genome Institute (Walnut Creek, CA). Additional information can be found at http://wfleabase.org/.

text: Adult

text: Daphnia pulex

text: Sfi I A

text: Sfi I B

text: Mixed

text: Log50 (arenata)

text: whole animal

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: Juvenile

text: Daphnia pulex

text: Sfi I A

text: Sfi I B

text: Mixed

text: Log50 (arenata)

text: whole animal

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: Stressed by metal exposure, high concentrations (20 ug Cd/L; 200 ug Zn/L; 1348 ug As/L) pooled into this single sample. The library was made from oligo(dT)-primed cDNA and cloned into vector pDNR-LIB (Clontech Laboratories, Inc., Mountain View, CA) using the SMART system (BD Biosciences, San Jose, CA). To generate the first strand, polyA RNA was primed with an oligo(dT) primer (5'-ATTCTAGAGGCCGAGGCGGCCGACATG(T)30 VN-3', where V = A, G, or C). The second strand was synthesized by PCR using primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' followed by digestion of the SfiI sites. cDNA was size selected using Sephadex columns provided with the SMART kit. To maximize the ligation efficiency of large transcripts, the largest size fraction was kept separate from the others. Library was created by the Daphnia Genomics Consortium and sequencing was done at DOE Joint Genome Institute (Walnut Creek, CA). Additional information can be found at http://wfleabase.org/.

text: Mixed

text: Daphnia pulex

text: Sfi I A

text: Sfi I B

text: Female

text: Log50 (arenata)

text: whole animal

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: Stressed by exposure to invertebrate predator (Chaoborus americanus). The library was made from oligo(dT)-primed cDNA and cloned into vector pDNR-LIB (Clontech Laboratories, Inc., Mountain View, CA) using the SMART system (BD Biosciences, San Jose, CA). To generate the first strand, polyA RNA was primed with an oligo(dT) primer (5'-ATTCTAGAGGCCGAGGCGGCCGACATG(T)30 VN-3', where V = A, G, or C). The second strand was synthesized by PCR using primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' followed by digestion of the SfiI sites. cDNA was size selected using Sephadex columns provided with the SMART kit. To maximize the ligation efficiency of large transcripts, the largest size fraction was kept separate from the others. Library was created by the Daphnia Genomics Consortium and sequencing was done at DOE Joint Genome Institute (Walnut Creek, CA). Additional information can be found at http://wfleabase.org/.

text: Adult

text: Daphnia pulex

text: Sfi I A

text: Sfi I B

text: Mixed

text: Log50 (arenata)

text: whole animal

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: Standard laboratory conditions. The library was made from oligo(dT)-primed cDNA and cloned into vector pDNR-LIB (Clontech Laboratories, Inc., Mountain View, CA) using the SMART system (BD Biosciences, San Jose, CA). To generate the first strand, polyA RNA was primed with an oligo(dT) primer (5'-ATTCTAGAGGCCGAGGCGGCCGACATG(T)30 VN-3', where V = A, G, or C). The second strand was synthesized by PCR using primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' followed by digestion of the SfiI sites. cDNA was size selected using Sephadex columns provided with the SMART kit. To maximize the ligation efficiency of large transcripts, the largest size fraction was kept separate from the others. Library is normalized. Library was created at the Center for Genomics and Bioinformatics, Indiana University and sequencing was done at DOE Joint Genome Institute (Walnut Creek, CA). Additional information can be found at http://wfleabase.org/.

text: Juvenile (4 days old)

text: Daphnia pulex

text: Sfi I A

text: Sfi I B

text: Strictly Female

text: The Chosen One (arenata)

text: whole animal

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: Adult (10 days old) with embryos in brooding chamber

text: Daphnia pulex

text: Sfi I A

text: Sfi I B

text: Strictly Female

text: The Chosen One (arenata)

text: whole animal

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: Adult (10 days old)

text: Daphnia pulex

text: Sfi I A

text: Sfi I B

text: Strictly Male

text: The Chosen One (arenata)

text: whole animal

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: Stressed by exposure to nickle (200 ug/L of Ni as nickle chloride). The library was made from oligo(dT)-primed cDNA and cloned into vector pDNR-LIB (Clontech Laboratories, Inc., Mountain View, CA) using the SMART system (BD Biosciences, San Jose, CA). To generate the first strand, polyA RNA was primed with an oligo(dT) primer (5'-ATTCTAGAGGCCGAGGCGGCCGACATG(T)30 VN-3', where V = A, G, or C). The second strand was synthesized by PCR using primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' followed by digestion of the SfiI sites. cDNA was size selected using Sephadex columns provided with the SMART kit. To maximize the ligation efficiency of large transcripts, the largest size fraction was kept separate from the others. Library is normalized. Library was created at the Center for Genomics and Bioinformatics, Indiana University and sequencing was done at DOE Joint Genome Institute (Walnut Creek, CA). Additional information can be found at http://wfleabase.org/.

text: Adults plus neonates up to third instar

text: Daphnia pulex

text: Sfi I A

text: Sfi I B

text: Female

text: The Chosen One (arenata)

text: whole animal

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: Stressed by exposure to copper (5 ug/L of Cu as copper sulphate). The library was made from oligo(dT)-primed cDNA and cloned into vector pDNR-LIB (Clontech Laboratories, Inc., Mountain View, CA) using the SMART system (BD Biosciences, San Jose, CA). To generate the first strand, polyA RNA was primed with an oligo(dT) primer (5'-ATTCTAGAGGCCGAGGCGGCCGACATG(T)30 VN-3', where V = A, G, or C). The second strand was synthesized by PCR using primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' followed by digestion of the SfiI sites. cDNA was size selected using Sephadex columns provided with the SMART kit. To maximize the ligation efficiency of large transcripts, the largest size fraction was kept separate from the others. Library is normalized. Library was created at the Center for Genomics and Bioinformatics, Indiana University and sequencing was done at DOE Joint Genome Institute (Walnut Creek, CA). Additional information can be found at http://wfleabase.org/.

text: Adults plus neonates up to third instar

text: Daphnia pulex

text: Sfi I A

text: Sfi I B

text: Female

text: The Chosen One (arenata)

text: whole animal

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: Stressed by acid (pH 6.0). The library was made from oligo(dT)-primed cDNA and cloned into vector pDNR-LIB (Clontech Laboratories, Inc., Mountain View, CA) using the SMART system (BD Biosciences, San Jose, CA). To generate the first strand, polyA RNA was primed with an oligo(dT) primer (5'-ATTCTAGAGGCCGAGGCGGCCGACATG(T)30 VN-3', where V = A, G, or C). The second strand was synthesized by PCR using primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' followed by digestion of the SfiI sites. cDNA was size selected using Sephadex columns provided with the SMART kit. To maximize the ligation efficiency of large transcripts, the largest size fraction was kept separate from the others. Library is normalized. Library was created at the Center for Genomics and Bioinformatics, Indiana University and sequencing was done at DOE Joint Genome Institute (Walnut Creek, CA). Additional information can be found at http://wfleabase.org/.

text: Adults plus neonates up to third instar

text: Daphnia pulex

text: Sfi I A

text: Sfi I B

text: Female

text: The Chosen One (arenata)

text: whole animal

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: Stressed by salt (750 mg/L of sodium chloride). The library was made from oligo(dT)-primed cDNA and cloned into vector pDNR-LIB (Clontech Laboratories, Inc., Mountain View, CA) using the SMART system (BD Biosciences, San Jose, CA). To generate the first strand, polyA RNA was primed with an oligo(dT) primer (5'-ATTCTAGAGGCCGAGGCGGCCGACATG(T)30 VN-3', where V = A, G, or C). The second strand was synthesized by PCR using primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' followed by digestion of the SfiI sites. cDNA was size selected using Sephadex columns provided with the SMART kit. To maximize the ligation efficiency of large transcripts, the largest size fraction was kept separate from the others. Library is normalized. Library was created at the Center for Genomics and Bioinformatics, Indiana University and sequencing was done at DOE Joint Genome Institute (Walnut Creek, CA). Additional information can be found at http://wfleabase.org/.

text: Adults plus neonates up to third instar

text: Daphnia pulex

text: Sfi I A

text: Sfi I B

text: Female

text: The Chosen One (arenata)

text: whole animal

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: Stressed by exposure to nanoparticles (Fullerene). The library was made from oligo(dT)-primed cDNA and cloned into vector pDNR-LIB (Clontech Laboratories, Inc., Mountain View, CA) using the SMART system (BD Biosciences, San Jose, CA). To generate the first strand, polyA RNA was primed with an oligo(dT) primer (5'-ATTCTAGAGGCCGAGGCGGCCGACATG(T)30 VN-3', where V = A, G, or C). The second strand was synthesized by PCR using primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' followed by digestion of the SfiI sites. cDNA was size selected using Sephadex columns provided with the SMART kit. To maximize the ligation efficiency of large transcripts, the largest size fraction was kept separate from the others. Library is normalized. Library was created at the Center for Genomics and Bioinformatics, Indiana University and sequencing was done at DOE Joint Genome Institute (Walnut Creek, CA). Additional information can be found at http://wfleabase.org/.

text: 3 day old neonates

text: Daphnia pulex

text: Sfi I A

text: Sfi I B

text: Female

text: The Chosen One (arenata)

text: whole animal

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: Stressed by bacterial infection. The library was made from oligo(dT)-primed cDNA and cloned into vector pDNR-LIB (Clontech Laboratories, Inc., Mountain View, CA) using the SMART system (BD Biosciences, San Jose, CA). To generate the first strand, polyA RNA was primed with an oligo(dT) primer (5'-ATTCTAGAGGCCGAGGCGGCCGACATG(T)30 VN-3', where V = A, G, or C). The second strand was synthesized by PCR using primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' followed by digestion of the SfiI sites. cDNA was size selected using Sephadex columns provided with the SMART kit. To maximize the ligation efficiency of large transcripts, the largest size fraction was kept separate from the others. Library is normalized. Library was created at the Center for Genomics and Bioinformatics, Indiana University and sequencing was done at DOE Joint Genome Institute (Walnut Creek, CA). Additional information can be found at http://wfleabase.org/.

text: Mixed including parthenogenetics and sexuals

text: Daphnia pulex

text: Sfi I A

text: Sfi I B

text: Female

text: The Chosen One (arenata)

text: whole animal

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: Stressed by mixed metals exposure, high concentrations (20 ug Cd/L; 200 ug Zn/L; 1348 ug As/L). The library was made from oligo(dT)-primed cDNA and cloned into vector pDNR-LIB (Clontech Laboratories, Inc., Mountain View, CA) using the SMART system (BD Biosciences, San Jose, CA). To generate the first strand, polyA RNA was primed with an oligo(dT) primer (5'-ATTCTAGAGGCCGAGGCGGCCGACATG(T)30 VN-3', where V = A, G, or C). The second strand was synthesized by PCR using primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' followed by digestion of the SfiI sites. cDNA was size selected using Sephadex columns provided with the SMART kit. To maximize the ligation efficiency of large transcripts, the largest size fraction was kept separate from the others. Library is normalized. Library was created at the Center for Genomics and Bioinformatics, Indiana University and sequencing was done at DOE Joint Genome Institute (Walnut Creek, CA). Additional information can be found at http://wfleabase.org/.

text: Adults plus neonates up to third instar

text: Daphnia pulex

text: Sfi I A

text: Sfi I B

text: Female

text: The Chosen One (arenata)

text: whole animal

text: CBTP_Daphnia_pulex_Chosen_One_Library_10

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: Stressed by mixed metals expsoure, low concentrations (1 ug Cd/L; 50 ug Zn/L; 500 ug As/L). The library was made from oligo(dT)-primed cDNA and cloned into vector pDNR-LIB (Clontech Laboratories, Inc., Mountain View, CA) using the SMART system (BD Biosciences, San Jose, CA). To generate the first strand, polyA RNA was primed with an oligo(dT) primer (5'-ATTCTAGAGGCCGAGGCGGCCGACATG(T)30 VN-3', where V = A, G, or C). The second strand was synthesized by PCR using primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' followed by digestion of the SfiI sites. cDNA was size selected using Sephadex columns provided with the SMART kit. To maximize the ligation efficiency of large transcripts, the largest size fraction was kept separate from the others. Library is normalized. Library was created at the Center for Genomics and Bioinformatics, Indiana University and sequencing was done at DOE Joint Genome Institute (Walnut Creek, CA). Additional information can be found at http://wfleabase.org/.

text: Adults plus neonates up to third instar

text: Daphnia pulex

text: Sfi I A

text: Sfi I B

text: Female

text: The Chosen One (arenata)

text: whole animal

text: CBTS_Daphnia_pulex_Chosen_One_Library_11

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: Stressed by exposure to monomethylarsenic III (100 ug/L of As as diiodo-MMAIII). The library was made from oligo(dT)-primed cDNA and cloned into vector pDNR-LIB (Clontech Laboratories, Inc., Mountain View, CA) using the SMART system (BD Biosciences, San Jose, CA). To generate the first strand, polyA RNA was primed with an oligo(dT) primer (5'-ATTCTAGAGGCCGAGGCGGCCGACATG(T)30 VN-3', where V = A, G, or C). The second strand was synthesized by PCR using primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' followed by digestion of the SfiI sites. cDNA was size selected using Sephadex columns provided with the SMART kit. To maximize the ligation efficiency of large transcripts, the largest size fraction was kept separate from the others. Library is normalized. Library was created at the Center for Genomics and Bioinformatics, Indiana University and sequencing was done at DOE Joint Genome Institute (Walnut Creek, CA). Additional information can be found at http://wfleabase.org/.

text: Adults plus neonates up to third instar

text: Daphnia pulex

text: Sfi I A

text: Sfi I B

text: Female

text: The Chosen One (arenata)

text: whole animal

text: CBTT_Daphnia_pulex_Chosen_One_Library_12

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: Stressed by exposure to nanoparticles (Titanium Dioxide). The library was made from oligo(dT)-primed cDNA and cloned into vector pDNR-LIB (Clontech Laboratories, Inc., Mountain View, CA) using the SMART system (BD Biosciences, San Jose, CA). To generate the first strand, polyA RNA was primed with an oligo(dT) primer (5'-ATTCTAGAGGCCGAGGCGGCCGACATG(T)30 VN-3', where V = A, G, or C). The second strand was synthesized by PCR using primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' followed by digestion of the SfiI sites. cDNA was size selected using Sephadex columns provided with the SMART kit. To maximize the ligation efficiency of large transcripts, the largest size fraction was kept separate from the others. Library is normalized. Library was created at the Center for Genomics and Bioinformatics, Indiana University and sequencing was done at DOE Joint Genome Institute (Walnut Creek, CA). Additional information can be found at http://wfleabase.org/.

text: 5 day old neonates

text: Daphnia pulex

text: Sfi I A

text: Sfi I B

text: Female

text: The Chosen One (arenata)

text: whole animal

text: CBTU_Daphnia_pulex_Chosen_One_Library_13

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: Stressed by exposure to Microcystis. The library was made from oligo(dT)-primed cDNA and cloned into vector pDNR-LIB (Clontech Laboratories, Inc., Mountain View, CA) using the SMART system (BD Biosciences, San Jose, CA). To generate the first strand, polyA RNA was primed with an oligo(dT) primer (5'-ATTCTAGAGGCCGAGGCGGCCGACATG(T)30 VN-3', where V = A, G, or C). The second strand was synthesized by PCR using primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' followed by digestion of the SfiI sites. cDNA was size selected using Sephadex columns provided with the SMART kit. To maximize the ligation efficiency of large transcripts, the largest size fraction was kept separate from the others. Library is normalized. Library was created at the Center for Genomics and Bioinformatics, Indiana University and sequencing was done at DOE Joint Genome Institute (Walnut Creek, CA). Additional information can be found at http://wfleabase.org/.

text: Adults plus neonates up to third instar

text: Daphnia pulex

text: Sfi I A

text: Sfi I B

text: Female

text: The Chosen One (arenata)

text: whole animal

text: CBTW_Daphnia_pulex_Chosen_One_Library_14

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: Stressed by calcium starvation (1 mg/L of Ca). The library was made from oligo(dT)-primed cDNA and cloned into vector pDNR-LIB (Clontech Laboratories, Inc., Mountain View, CA) using the SMART system (BD Biosciences, San Jose, CA). To generate the first strand, polyA RNA was primed with an oligo(dT) primer (5'-ATTCTAGAGGCCGAGGCGGCCGACATG(T)30 VN-3', where V = A, G, or C). The second strand was synthesized by PCR using primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' followed by digestion of the SfiI sites. cDNA was size selected using Sephadex columns provided with the SMART kit. To maximize the ligation efficiency of large transcripts, the largest size fraction was kept separate from the others. Library is normalized. Library was created at the Center for Genomics and Bioinformatics, Indiana University and sequencing was done at DOE Joint Genome Institute (Walnut Creek, CA). Additional information can be found at http://wfleabase.org/.

text: Adults plus neonates up to third instar

text: Daphnia pulex

text: Sfi I A

text: Sfi I B

text: Female

text: The Chosen One (arenata)

text: whole animal

text: CBTX_Daphnia_pulex_Chosen_One_Library_15

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: Stressed by starvation. The library was made from oligo(dT)-primed cDNA and cloned into vector pDNR-LIB (Clontech Laboratories, Inc., Mountain View, CA) using the SMART system (BD Biosciences, San Jose, CA). To generate the first strand, polyA RNA was primed with an oligo(dT) primer (5'-ATTCTAGAGGCCGAGGCGGCCGACATG(T)30 VN-3', where V = A, G, or C). The second strand was synthesized by PCR using primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' followed by digestion of the SfiI sites. cDNA was size selected using Sephadex columns provided with the SMART kit. To maximize the ligation efficiency of large transcripts, the largest size fraction was kept separate from the others. Library was created by the Daphnia Genomics Consortium and sequencing was done at DOE Joint Genome Institute (Walnut Creek, CA). Additional information can be found at http://wfleabase.org/.

text: Adult

text: Daphnia pulex

text: Sfi I A

text: Sfi I B

text: Mixed

text: Log50 (arenata)

text: whole animal

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: Juvenile

text: Daphnia pulex

text: Sfi I A

text: Sfi I B

text: Mixed

text: Log50 (arenata)

text: whole animal

text: pDNR-LIB


text: University of Notre Dame

text: live

text: Generic

text: Oenocytes

characteristic: cell type

text: Pupae

text: fat body

characteristic: organ

text: Aedes aegypti

text: male and female

text: MOYO-R

text: oenocytes

text: Aedes agegypti MOYO-R pupae oenocytes library


text: China Agriculture University

text: live

text: Generic

text: Adult

text: Chrysoperla nipponensis

text: EcoRI

text: XhoI

text: Male and female

text: All body

characteristic: tissue-type

text: cDNA library of Chrysoperla nipponensis

text: pBluescript SK(+)XR

characteristic: vector


characteristic: External Id

text: University of Antwerp

text: live

text: Generic

text: Cyprinus carpio

text: liver

text: Cyprinus carpio endocrine disruption related cDNA library


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT)-primed cDNA and cloned into the pMCL200_cdna vector. PolyA RNA was primed with oligo(dT) primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'-CGGACGCGTGGG) and digested with NotI. cDNA was size selected >2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pMCL200_cDNA vector. Library was created and sequencing done by the DOE Joint Genome Institute (Walnut Creek, CA).

text: mixed

characteristic: dev-stage

text: Selaginella moellendorffii

text: SalI

text: NotI

text: Plant Delights

text: CAOP Selaginella moellendorffii mixed tissues; whole juvenile plants with roots and reproductive stems with developing strobili


text: DOE Joint Genome Institute

characteristic: INSDC center name

text: live

text: Generic

text: The library was made from oligo(dT)-primed cDNA and cloned into the pMCL200_cdna vector. PolyA RNA was primed with oligo(dT) primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'-CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 0.6-2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pMCL200_cDNA vector. Library was created and sequencing done by the DOE Joint Genome Institute (Walnut Creek, CA).

text: mixed

text: Selaginella moellendorffii

text: SalI

text: NotI

text: Plant Delights

text: CAOS Selaginella moellendorffii mixed tissues; whole juvenile plants with roots and reproductive stems with developing strobili


text: DOE Joint Genome Institute

characteristic: INSDC first public

text: live

text: Generic

text: mixed

text: Selaginella moellendorffii

text: SalI

text: NotI

text: Plant Delights

text: CAOT Selaginella moellendorffii mixed tissues; whole juvenile plants with roots and reproductive stems with developing strobili


text: DOE Joint Genome Institute

characteristic: INSDC last update

text: live

text: Generic

text: mixed

text: Selaginella moellendorffii

text: SalI

text: NotI

text: Plant Delights

text: CAOU Selaginella moellendorffii mixed tissues; whole juvenile plants with roots and reproductive stems with developing strobili


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected >2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. Library was created and sequencing done by the DOE Joint Genome Institute (Walnut Creek, CA).

text: mixed

text: Selaginella moellendorffii

text: SalI

text: NotI

text: Plant Delights

text: CAOW Selaginella moellendorffii mixed tissues; whole juvenile plants with roots and reproductive stems with developing strobili


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 1.2-2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. Library was created and sequencing done by the DOE Joint Genome Institute (Walnut Creek, CA).

text: mixed

text: Selaginella moellendorffii

text: SalI

text: NotI

text: Plant Delights

text: CAOX Selaginella moellendorffii mixed tissues; whole juvenile plants with roots and reproductive stems with developing strobili


text: DOE Joint Genome Institute

text: live

text: Generic

text: mixed

text: Selaginella moellendorffii

text: SalI

text: NotI

text: Plant Delights

text: CAOY Selaginella moellendorffii mixed tissues; whole juvenile plants with roots and reproductive stems with developing strobili


text: DOE Joint Genome Institute

text: live

text: Generic

text: mixed

text: Selaginella moellendorffii

text: SalI

text: NotI

text: Plant Delights

text: CAOZ Selaginella moellendorffii mixed tissues; whole juvenile plants with roots and reproductive stems with developing strobili


text: CSIRO Plant Industry

text: live

text: Generic

text: collected_by Li,X.; Wu,H.;Dillon,S.;Southerton,S. Developing xylem tissues from 7 yrs old trees in radiata pine were collected in spring, by scraping the partly-lignified thin layer on the debarked area at breast height. 5 mg of Poly(A)+ mRNA was used for cDNA library construction by using ZAP-cDNA Gigapack III Gold Cloning kit. The double strand cDNAs were fractionated by drip column, then the fractions at approximately 600 bp or more were pooled and ligated into cloning site of EcoRI/XhoI in Uni-ZAP XR vector. 1~2 ul of ligated cDNA inserts was packaged with Gigapack III Gold packaging extract. The pBluescript phagemids containing cDNA inserts were mass excised in vivo from Uni-ZAP XR vector by using Ex-Assist helper phage and XL1-Blue MRF.

text: Juvenile earlywood, at 7 yrs old and in spring

text: CSIRO Forest Biosciences

characteristic: lab-host

text: Pinus radiata

text: EcoRI

text: XhoI

text: Developing xylem

text: Radiata pine juvenile earlywood cDNA library

text: Phage

text: Uni-ZAP XR


text: CSIRO Plant Industry

text: live

characteristic: INSDC status

text: Generic

text: collected_by Li,X.; Wu,H.;Dillon,S.;Southerton,S. Developing xylem tissues from 7 yrs old trees in radiata pine were collected in autumn, by scraping the partly-lignified thin layer on the debarked area at breast height. 5 mg of Poly(A)+ mRNA was used for cDNA library construction by using ZAP-cDNA Gigapack III Gold Cloning kit. The double strand cDNAs were fractionated by drip column, then the fractions at approximately 600 bp or more were pooled and ligated into cloning site of EcoRI/XhoI in Uni-ZAP XR vector. 1~2 ul of ligated cDNA inserts was packaged with Gigapack III Gold packaging extract. The pBluescript phagemids containing cDNA inserts were mass excised in vivo from Uni-ZAP XR vector by using Ex-Assist helper phage and XL1-Blue MRF.

text: Juvenile latewood, at 7 yrs old and in autumn

text: CSIRO Forest Biosciences

text: Pinus radiata

text: EcoRI

text: XhoI

text: Developing xylem

text: Radiata pine juvenile latewood cDNA library

text: Phage

text: Uni-ZAP XR


text: CSIRO Plant Industry

text: live

text: Generic

characteristic: NCBI submission model

text: collected_by Li,X.; Wu,H.;Dillon,S.;Southerton,S. Developing xylem tissues from ~30 yrs old trees in radiata pine were collected in spring, by scraping the partly-lignified thin layer on the debarked area at breast height. 5 mg of Poly(A)+ mRNA was used for cDNA library construction by using ZAP-cDNA Gigapack III Gold Cloning kit. The double strand cDNAs were fractionated by drip column, then the fractions at approximately 600 bp or more were pooled and ligated into cloning site of EcoRI/XhoI in Uni-ZAP XR vector. 1~2 ul of ligated cDNA inserts was packaged with Gigapack III Gold packaging extract. The pBluescript phagemids containing cDNA inserts were mass excised in vivo from Uni-ZAP XR vector by using Ex-Assist helper phage and XL1-Blue MRF.

text: Mature earlywood, at ~30 yrs old and in spring

text: CSIRO Forest Biosciences

text: Pinus radiata

text: EcoRI

text: XhoI

text: Developing xylem

text: Radiata pine mature earlywood cDNA library

text: Phage

text: Uni-ZAP XR


text: CSIRO Plant Industry

text: live

text: Generic

characteristic: NCBI submission package

text: collected_by Li,X.; Wu,H.;Dillon,S.;Southerton,S. Developing xylem tissues from ~30 yrs old trees in radiata pine were collected in autumn, by scraping the partly-lignified thin layer on the debarked area at breast height. 5 mg of Poly(A)+ mRNA was used for cDNA library construction by using ZAP-cDNA Gigapack III Gold Cloning kit. The double strand cDNAs were fractionated by drip column, then the fractions at approximately 600 bp or more were pooled and ligated into cloning site of EcoRI/XhoI in Uni-ZAP XR vector. 1~2 ul of ligated cDNA inserts was packaged with Gigapack III Gold packaging extract. The pBluescript phagemids containing cDNA inserts were mass excised in vivo from Uni-ZAP XR vector by using Ex-Assist helper phage and XL1-Blue MRF.

text: Mature latewood, at ~30 yrs old and in autumn

text: CSIRO Forest Biosciences

text: Pinus radiata

text: EcoRI

text: XhoI

text: Developing xylem

text: Radiata pine mature latewood cDNA library

text: Phage

text: Uni-ZAP XR


text: CSIRO Plant Industry

text: live

text: Generic

text: collected_by Li,X.; Wu,H.;Dillon,S.;Southerton,S. Developing xylem tissues from 11 yrs old trees in radiata pine were collected in spring, by scraping the partly-lignified thin layer on the debarked area at breast height. 5 mg of Poly(A)+ mRNA was used for cDNA library construction by using ZAP-cDNA Gigapack III Gold Cloning kit. The double strand cDNAs were fractionated by drip column, then the fractions at approximately 600 bp or more were pooled and ligated into cloning site of EcoRI/XhoI in Uni-ZAP XR vector. 1~2 ul of ligated cDNA inserts was packaged with Gigapack III Gold packaging extract. The pBluescript phagemids containing cDNA inserts were mass excised in vivo from Uni-ZAP XR vector by using Ex-Assist helper phage and XL1-Blue MRF.

text: Transition earlywood, at 11 yrs old and in spring

text: CSIRO Forest Biosciences

text: Pinus radiata

text: EcoRI

text: XhoI

text: Developing xylem

text: Radiata pine transition earlywood cDNA library

text: Phage

text: Uni-ZAP XR


text: CSIRO Plant Industry

text: live

text: Generic

text: collected_by Li,X.; Wu,H.;Dillon,S.;Southerton,S. Developing xylem tissues from 11 yrs old trees in radiata pine were collected in autumn, by scraping the partly-lignified thin layer on the debarked area at breast height. 5 mg of Poly(A)+ mRNA was used for cDNA library construction by using ZAP-cDNA Gigapack III Gold Cloning kit. The double strand cDNAs were fractionated by drip column, then the fractions at approximately 600 bp or more were pooled and ligated into cloning site of EcoRI/XhoI in Uni-ZAP XR vector. 1~2 ul of ligated cDNA inserts was packaged with Gigapack III Gold packaging extract. The pBluescript phagemids containing cDNA inserts were mass excised in vivo from Uni-ZAP XR vector by using Ex-Assist helper phage and XL1-Blue MRF.

text: Transition latewood, at 11 yrs old and in autumn

text: CSIRO Forest Biosciences

text: Pinus radiata

text: EcoRI

text: XhoI

text: Developing xylem

text: Radiata pine transition latewood cDNA library

text: Phage

text: Uni-ZAP XR


text: Faculdade de Medicina de Ribeirio Preto da Universidade de Sao Paulo

text: live

text: Generic

text: T.rubrum developmental phase transcripts.

text: E.coli TOP10

text: Trichophyton rubrum

characteristic: strain

text: Trichophyton rubrum cDNA Library NMMR01

text: Plasmid

text: pDNR-LIB


text: live

text: Generic

text: Cytotoxic agents response-enriched transcripts.

text: E.coli MOS Blue

text: Trichophyton rubrum

characteristic: organism

text: Trichophyton rubrum SSH Library NMMR02

text: Plasmid


text: live

text: Generic

text: AMB response-enriched transcripts.

text: E.coli MOS Blue

text: Trichophyton rubrum

text: Trichophyton rubrum SSH Library NMMR03

characteristic: title

text: Plasmid

text: pGEM-T


text: live

text: Generic

text: Fluconazole resistance-enriched transcripts.

characteristic: description

text: E.coli MOS Blue

text: Trichophyton rubrum

text: Trichophyton rubrum SSH Library NMMR04

text: Plasmid

text: pGEM-T


text: live

text: Generic

text: Fluconazole resistance-repressed transcripts.

text: E.coli MOS Blue

text: Trichophyton rubrum

text: Trichophyton rubrum SSH Library NMMR05

text: Plasmid

text: pGEM-T


text: live

text: Generic

text: Glucose-enriched transcripts.

text: E.coli MOS Blue

text: Trichophyton rubrum

text: Trichophyton rubrum SSH Library NMMR06

text: Plasmid

text: pGEM-T


text: live

text: Generic

text: Keratin-enriched transcripts.

text: E.coli MOS Blue

text: Trichophyton rubrum

text: Trichophyton rubrum SSH Library NMMR07

text: Plasmid

text: pGEM-T


text: live

text: Generic

text: Transcripts over-expressed in T.rubrum submetted to pH stress - A-30min.

text: E.coli MOS Blue

text: Trichophyton rubrum

text: Trichophyton rubrum SSH Library NMMR08

text: Plasmid

text: pGEM-T


text: live

text: Generic

text: Transcripts over-expressed in T.rubrum submetted to pH stress - B-60min.

text: E.coli MOS Blue

text: Trichophyton rubrum

text: Trichophyton rubrum SSH Library NMMR09

text: Plasmid

text: pGEM-T


text: live

text: Generic

text: Transcripts over-expressed in T.rubrum submetted to pH stress - C.

text: E.coli MOS Blue

text: Trichophyton rubrum

text: Trichophyton rubrum SSH Library NMMR10

text: Plasmid

text: pGEM-T


text: Institute of Botany, Chinese Academy of Sciences (IBCAS)

text: live

text: Generic

text: The preparation of library was according to the protocol provided by CreatorTM SMART TM cDNA library construction kit, using SMARTTM cDNA synthesis by LD-PCR for obtaining enough cDNA (Protocol No.PT3577-1, Version No. PR651803). Vector was provided by producer and cut by Sfi I A and Sfi I B. Primers: PCR forward: 5' GTA AAA CGA CGG CCA GT3'; PCR backward: 5' AAA CAG CTA TGA CCA TGT TCA 3'

text: all developmental stages

text: Ecoli DH5alpha

text: Part of leaf, all flower organs

text: Paeonia suffruticosa

text: Sfi I A (5' side of insert)

text: Sfi I B (3' side of insert)

text: hermaphrodite

characteristic: sex

text: Flower buds

text: Flower Buds of Tree Peony

text: Plasmid

text: pDNR-LIB


text: Centre de Regulacio Genomica

text: live

text: Generic

text: Homo sapiens

text: RT-PCR of adult human tissue RNAs


text: Bioengineering College, Chongqing University

text: live

text: Generic

text: The subtractive hybridization protocol from BD Bioscience Clontech (Palo Alto, CA) has been used to generate this specific cDNA library. Tester cDNA was prepared from conidial cultured 28h and driver cDNA was prepared from conidial cultured 20h. The resulting cDNAs were cloned into PMD-18T vector.

text: Metarhizium acridum

text: conidiogenous cell stage SSH Library


text: North Dakota State University

text: live

text: Generic

text: The library was constructed using the SMART cDNA Library Construction Kit (DB-Bioscience-ClonTech, Palo Alto, CA) and the Gateway (Invitrogen, Carlsbad, CA) cloning system with modifications from manufacturer's protocols. Briefly, for first-strand cDNA synthesis, we used the attB2-oligo-dT primer (GGGGACCACTTTGTACAAGAAAGCTGGGTTTTTTTTTTTTTTTTTTTTVN) and attB1-Switch primer (GGGGACAAGTTTGTACAAAAAAGCAGGCTrGrGrG) for the first strand synthesis. The first strand was further amplified with primers attB1 (GGGGACAAGTTTGTACAAAAAAGCAGGCT) and attB2 (GGGGACCACTTTGTACAAGAAAGCTGGGT). The final products were cloned into the plasmid pDONR221 by recombination using BP clonase as described in the manufacturer's protocol (Invitrogen, Carlsbad, CA). The plasmids were transformed in DH10B E. coli strain.

text: DH10B E. coli

text: Pseudocercospora fijiensis

text: mycelia

text: FL, mycelia grown in Flies medium at room temperature

text: plasmid


text: Aristotle University of Thessaloniki

text: live

text: Generic

text: Produced with CreatorTM SMARTTM cDNA Library Construction Kit

text: Salvia fruticosa

text: Sfi A

text: Sfi B

text: leaves trichomes

text: Library from leaves trichomes of Salvia fruticosa

text: Plasmid

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: cultured. The library was made from oligo(dT)-primed cDNA and cloned into vector pDNR-LIB (Clontech Laboratories, Inc., Mountain View, CA) using the SMART system (BD Biosciences, San Jose, CA). To generate the first strand, polyA RNA was primed with an oligo(dT) primer (5'-ATTCTAGAGGCCGAGGCGGCCGACATG(T)30 VN-3', where V = A, G, or C). The second strand was synthesized by PCR using primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' followed by digestion of the SfiI sites. cDNA was size selected from 2-3kb using 1.1% agarose gel electrophoresis, then ligated into pDNR-LIB. Library was created and sequencing performed by the DOE Joint Genome Institute (Walnut Creek, CA).

text: Symbiodinium sp. KB8

text: culture

text: CAXB Symbiodinium sp. KB8

text: pDNR-LIB


text: The Breed & Biotechnology Key Laboratory of Sheep

text: live

text: Generic

text: immature proglottid of adult

text: Moniezia benedeni

text: Shihezi

text: Moniezia benedeni Shihezi immature proglottids


text: Chung-Ang University

text: live

text: Generic

text: Perionyx excavatus

text: EcoRI

text: XhoI

text: regenerating tissue

text: Perionyx excavatus regenerating tissue cDNA library

text: pCMV-Script XR


text: Tokyo University of Marine Science and Technology

text: live

text: Generic

text: Cells isolated from tissue were treated with ConA and LPS. After incubation for several hours, the cells were harvested and used for cDNA library construction.

text: Seriola quinqueradiata

text: spleen

text: Seriola quinqueradiata spleen


text: live

text: Generic

text: Seriola quinqueradiata

text: kidney

text: Seriola quinqueradiata kidney cells


text: Universite Paris-Sud

text: live

text: Generic

text: isolate from Silene nutans

text: Microbotryum violaceum

text: LB

text: Microbotryum violaceum LB

text: pGEM(R)-T


text: Universite Paris-Sud

text: live

text: Generic

text: isolate from Silene vulgaris (MvSv1)

text: Microbotryum violaceum

text: Microbotryum violaceum 300.27

text: pGEM(R)-T


text: Universite Paris-Sud

text: live

text: Generic

text: isolate from Dianthus carthusianorum

text: Microbotryum violaceum

text: Microbotryum violaceum 309.5

text: pGEM(R)-T


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT)-primed cDNA and cloned into Invitrogen vector pDONR222 (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with a biotin-attB2-Oligo(dT) primer (5'-GGCGGCCGCACAACTTTGTACAAGAAAGTTGGGT(T)19-3') and the first strand synthesized using Superscript II (Invitrogen Corp., Carlsbad, CA). The second strand was synthesized and an attB1 adaptor ligated to the 5' end (5'-TCGTCGGGGACAACTTTGTACAAAAAAGTTGG-3' and 5'-CCAACTTTTTTGTACAAAGTTGTCCCC-3'). cDNA was size selected using column chromatography and then inserted into the vector using site specific recombination (flanking attB sites on cDNA). RNA prepared at University of California, San Diego, library constructed at Kyoto University (Kyoto, Japan), and sequencing was done at DOE Joint Genome Institute (Walnut Creek, CA).

text: neurula

text: Branchiostoma floridae

text: whole animal

text: Amphioxus Branchiostoma floridae unpublished cDNA library CAXC, neurula whole animal


text: DOE Joint Genome Institute

text: live

text: Generic

text: gastrula

text: Branchiostoma floridae

text: whole animal

text: Amphioxus Branchiostoma floridae unpublished cDNA library CAXF, gastrula whole animal


text: DOE Joint Genome Institute

text: live

text: Generic

text: larval

text: Branchiostoma floridae

text: whole animal

text: Amphioxus Branchiostoma floridae unpublished cDNA library CAXG, larva whole animal


text: College of Animal Science and Technology, Hunan Agricultural University

text: live

text: Generic

text: ESTs related to Piglet's Escherichia coli F4 Receptor by using differential display RT-PCR

text: liver

characteristic: organ

text: Sus scrofa

text: Yorkshire (Large white)

text: liver

text: Piglet, disease resistance


text: Div. of Crop Protection, Central Research Institute for Jute and Allied Fibres

text: live

text: Generic

text: Patharkuchi

characteristic: cultivar

text: Apical leaf (3rd from top) of tomato plants (15 days old) were mechanically inoculated with the partially clarified sap obtained from leaves of rice (Oryza sativa cv. Khitish) plants infected with RNMV (Rice necrosis mosaic virus). When enlargement in leaf size as compared to control was noticed in inoculated plants, apical leaves were collected and frozen. Total RNA was isolated from the frozen leaves and subtractive hybridization was carried out. Final PCR product containing only the upregulated genes in response to inoculation was cloned in pJET 1.2 vector and sequenced.

text: Newly emerging leaves

text: Solanum lycopersicum

text: Leaves

characteristic: tissue-type

text: Library of tomato plant treated with RNMV infected rice leaf sap


characteristic: External Id

text: National Institute of Nutrition and Seafood Research

text: live

text: Generic

text: Sequenced PCR-product from rainbow trout exposed to atrazine

text: Adult

text: Oncorhynchus mykiss

text: Liver

text: Rainbow trout exposed to atrazine


text: DOE Joint Genome Institute

text: live

text: Generic

text: Kanlow

text: In Vivo Conditions: dark grown at 28C. Library was made from oligo(dT)-primed cDNA and cloned into the pSPORT1 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with oligo dT primer (5'- GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT -3'), ligated to a SalI adapter (5'- TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. Library is non-amplified. Tissue was provided by C. Tobias (USDA ARS, Albany, CA), library was constructed in the laboratory of P. Twigg (University of Nebraska, Kearney) and sequencing was performed at the DOE Joint Genome Institute (Walnut Creek, CA).

text: embryogenic and non-embrogenic

characteristic: dev-stage

text: DH5alpha FT

text: callus

text: Panicum virgatum

text: SalI

text: NotI

text: CBYX Panicum virgatum Kanlow callus

characteristic: vector


text: DOE Joint Genome Institute

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: Kanlow

text: Library was made from oligo(dT)-primed cDNA and cloned into the pSPORT1 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with oligo dT primer (5'- GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT -3'), ligated to a SalI adapter (5'- TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. Library is non-amplified. Tissue was provided by G. Sarath (USDA ARS, Albany, CA), library was constructed in the laboratory of P. Twigg (University of Nebraska, Kearney) and sequencing was performed at the DOE Joint Genome Institute (Walnut Creek, CA).

text: 6-8 wk plants

text: DH5alpha FT

text: crown

text: Panicum virgatum

text: SalI

text: NotI

text: CBYY Panicum virgatum Kanlow crown


text: DOE Joint Genome Institute

characteristic: INSDC last update

text: live

text: Generic

text: Kanlow

text: DH5alpha FT

characteristic: lab-host

text: whole seedlings

text: Panicum virgatum

text: SalI

text: NotI

text: CBYZ Panicum virgatum Kanlow 4 day old seedlings


text: Huazhong Agricultural University

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Hong Anliu

text: Library constructed using Clontech PCR-Select cDNA Subtraction kit.

text: Citrus sinensis

text: hermaphrodite

text: fruit

text: 'Hong Anliu' sweet orange SSH library


text: Institute of Plant Protection, Chinese Academy of Agricultural Sciences

text: live

text: Generic

characteristic: NCBI submission package

text: For study the expression of disease resistance genes of upland cotton induced by Prodenia litura, the cDNA library was constructed by suppression subtractive hybridization (SSH) based on the material induced by Prodenia litura after 24h 48h and 72h as a tester, the material of control as a driver.

text: six-leaf-old cotton plants

text: Gossypium hirsutum

text: leaf

text: Library for cotton induced by herbivores


text: United States Dept of Agriculture

text: live

text: Generic

text: Haustoria isolated from 5 dpi bean leaves

text: Phaseolus vulgaris cv. Pinto 111

text: Uromyces appendiculatus

characteristic: organism

text: SfiI

text: SfiI

text: Race 41

characteristic: strain

text: Plasmid

text: pDNR-LIB


text: Nanjing Agricultural University

text: live

text: Generic

text: Chickpea seedling was treated by 60 mM PEG 4000

characteristic: description

text: 10 days old

text: E. coli (DH10B)

text: Cicer arietinum

text: EcoRI

text: XhoI

text: hermaphrodite

text: leaf

text: Drought-stressed chickpea leave library

characteristic: title

text: plasmid

text: pBluescript II


text: Nanjing Agricultural University

text: live

text: Generic

text: 10 days old

text: E. coli (DH10B)

text: Cicer arietinum

text: EcoRI

text: XhoI

text: hermaphrodite

characteristic: sex

text: leaf

text: Chickpea leave library

text: plasmid

text: pBluescript II


text: Shanghai Fisheries University

text: live

text: Generic

text: Directionally cloned normalised full-length cDNA library constructed using poly A+ RNA from Myrmecia incisa

text: Lobosphaera incisa

text: Sfi I

text: Myrmecia incisa cDNA library

text: Phage

text: pTriplEx2 (Clontech)


text: School of Life Sciences, Hubei University

text: live

text: Generic

text: Nilaparvata lugens

text: Nilaparvata lugens library (Yang Z)


text: live

text: Generic

text: Parnara guttata

text: Parnara guttata library (Yang Z)


text: National Institute of Vegetable and Tea Science

text: live

text: Generic

text: Sayamakaori

characteristic: cultivar

text: Camellia sinensis

text: Camellia sinensis cv. Sayamakaori library


text: Max Planck Institute for Chemical Ecology

text: live

text: Generic

text: Backcross progeny from Family 315 were genotyped at the Ha-hyd marker linked to the fenvalerate resistance gene RFen1 to identify RS and SS individuals. Total RNA isolated from individual larvae was pooled into RS and SS bulks and cDNA-AFLP differences were identified by polyacrylamide gel electrophoresis. Differentially expressed fragments were excised, re-amplified and sequenced.

text: third instar larva

text: Helicoverpa armigera

text: entire larva

characteristic: tissue-type

text: Helicoverpa armigera AN02 F315 RFen1 RS/SS pools


characteristic: External Id

text: University of Pisa

text: live

text: Generic

text: Platanus x hispanica

text: Leaf

text: Platanus x acerifolia Leaf