characteristic: External Id

text: Institut Pasteur de Lille

text: live

text: Generic

text: mRNA was harvested from Echinostoma caproni infected B. glabrata at 24h, 48h and 96h post exposure, and from control snails at 24h, 48h and 96h after sham exposure. Combined cDNAs from parasite exposed and unexposed snails were subtracted using the PCR-Select Suppression Subtractive Hybridization kit (SSH) (Clontech, Palo Alto, CA). The resulting library was cloned into pCR4(r)-TOPO(r) (Invitrogen, Carlsbad, CA) and sequenced using the forward plasmid-specific primer T7

text: Adult

characteristic: dev-stage

text: Whole body

text: Biomphalaria glabrata

text: hermaphrodite

text: Bg.CB

characteristic: strain

text: B. glabrata SSH EST library

text: Plasmid


status: PUBLIC

text: NFSPRC Bldg.

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Subtractive hybridization library derived from soil grown, 500 mM NaCl treated, Lolium temulentum plants. Plant leaves and crowns were harvested 20 hours after stress. Products were TA cloned into vector and sequenced with T7 Primer.

characteristic: description

text: adult, nonflowering, plants grown under short days

tag: attribute

text: E. coli, Top010

characteristic: lab-host

text: Lolium temulentum

characteristic: organism

text: leaf and crown

characteristic: tissue-type

text: Lolium temulentum salt stressed subtraction library

characteristic: title

text: Plasmid

text: pGEM T easy

characteristic: vector


text: Technische Universitaet Muenchen

text: live

text: Generic

text: Golden Delicious, Rewena

characteristic: cultivar

text: cDNA library highly enriched for Venturia inaequalis induced genes.

text: leaf

characteristic: organ

text: Malus domestica

text: apple lib containing V. inaequalis induced genes


characteristic: External Id

text: USDA-ARS

text: live

text: Generic

text: Differential Display. First strand cDNA synthesized by RT-PCR from mRNA expressed in poplar trees (approximately 60cm tall) that were either mechanically wounded or infested with gypsy moth larvae. Mechanical wounding was performed by applying ~15 plier 'bytes' per leaf at 0, 2, and 4 hours and leaves harvested at 6 hours. For infestaction studies, sylleptic branches were infested with 25 third and fourth instar gypsy moth larvae (starved for 21 hours before) for 1 hour. The larvae were removed and the leaves harvested 3 hours later.

text: LPI 6-15

text: Leaf

text: Populus trichocarpa x Populus deltoides

text: HinD III

text: HinD III

text: Male

characteristic: sex

text: wound or herbivory regulated poplar library

text: Plasmid

text: pGEMT


text: Universite du Quebec a Montreal

text: live

text: Generic

text: Norstar

text: cDNAs were directionally cloned with Sal I adaptor (GTCGACCCACGCGTCCG) on the 5' end and Not I primer-adaptor (GCGGCCGCCC(T15)) on the 3' end.

text: Triticum aestivum

text: Crown and leaf

text: Genes amplified from wheat FGAS cDNA libraries (pExp)

text: pExp


text: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences

text: live

text: Generic

text: The total RNA was isolated from the spleen samples using TRIZOL Kit according to the manufacturer's protocol and digested with RNase-free DNase I to eliminate chromosomal DNA. The concentration of RNA was determined by spectrophotometer. A RNA pool containing equal amounts RNA of 20 chickens samples was established. Each RNA pool was reverse transcribed with M-MLV reverse transcriptase using one of the three anchor primers. The cDNA of the two samples were amplified with random primer and corresponding anchor primer. For the DDRT-PCR combinations of the 3 anchor primers together with 18 random primers. The DNA bands were separated on a polyacrylanlid gels and identified by silver staining. The differentially expressed bands were extracted from the gel and reamplified using the same primers set previously. The PCR products were identified by reverse Northern dot bloting with the DIG-labeled cDNA using DIG High Prime DNA Labeling and Detection Kit according to the manufacturer's protoco1. The positive DNA fragments were cloned into pGEM-T easy vector and then transformed into E.coli DH5a competent cells. After identification by enzyme digestion, the positive clones from the ampicillin positive LB plate were selected and sequenced. Uncertain fragment were identified by semiquantitative RT-PCR.

text: spleen

text: Gallus gallus

text: spleen

text: ARBOR ACRES spleen

text: pGEM-T easy


text: The Hebrew University of Jerusalem

text: live

text: Generic

text: Hales Best Jumbo

text: Library constructed using Clontech SMART cDNA library construction kit More information is available at http://melon.bti.cornell.edu

text: Cucumis melo

text: 5' Sfi IA

text: 3' Sfi IB

text: phloem sap stem collected between the 4th leaf and the 6th leaf from plants bearing fruits

text: Melon phloem library F

text: Plasmid

text: pTriplEx2 (excised from TriplEx2)


text: The Hebrew University of Jerusalem

text: live

text: Generic

text: Hales Best Jumbo

text: Cucumis melo

text: 5' Sfi IA

text: 3' Sfi IB

text: phloem sap stem collected between the 4th leaf and the 6th leaf from plants not bearing fruits

text: Melon phloem library UN

text: Plasmid

text: pTriplEx2 (excised from TriplEx2)


text: Max-Planck-Institute for Terrestrial Microbiology

text: live

text: Generic

text: germination

characteristic: dev-stage

text: Gigaspora gigantea

text: presymbiotic hyphae

text: Gigaspora gigantea presymbiotic hyphae germination


text: INRA - SCRIBE, Campus de beaulieu

characteristic: INSDC center name

text: live

text: Generic

text: Oncorhynchus mykiss

text: gill

text: Rainbow trout gill SSH library forward from salinity stress (tcag)

text: plasmid

text: pT-Adv


text: INRA - SCRIBE, Campus de beaulieu

characteristic: INSDC first public

text: live

text: Generic

text: Oncorhynchus mykiss

text: gill

text: Rainbow trout gill SSH library reverse from salinity stress (tcah)

text: plasmid

text: pT-Adv


text: INRA - SCRIBE, Campus de beaulieu

characteristic: INSDC last update

text: live

text: Generic

text: Oncorhynchus mykiss

text: brain

text: Rainbow trout brain SSH library forward from salinity stress (tcai)

text: plasmid

text: pT-Adv


text: INRA - SCRIBE, Campus de beaulieu

text: live

characteristic: INSDC status

text: Generic

text: Oncorhynchus mykiss

text: brain

text: Rainbow trout brain SSH library reverse from salinity stress (tcaj)

text: plasmid

text: pT-Adv


text: INRA - SCRIBE, Campus de beaulieu

text: live

text: Generic

characteristic: NCBI submission model

text: Oncorhynchus mykiss

text: head kidney

text: Rainbow trout head kidney SSH library reverse from confinement stress (tcak)

text: plasmid

text: pT-Adv


text: INRA - SCRIBE, Campus de beaulieu

text: live

text: Generic

characteristic: NCBI submission package

text: Oncorhynchus mykiss

text: head kidney

text: Rainbow trout head kidney SSH library forward from confinement stress (tcal)

text: plasmid

text: pT-Adv


text: INRA - SCRIBE, Campus de beaulieu

text: live

text: Generic

text: Oncorhynchus mykiss

text: skin

text: Rainbow trout skin SSH library reverse from confinement stress (tcam)

text: plasmid

text: pT-Adv


text: INRA - SCRIBE, Campus de beaulieu

text: live

text: Generic

text: Oncorhynchus mykiss

text: skin

text: Rainbow trout skin SSH library forward from confinement stress (tcan)

text: plasmid

text: pT-Adv


text: IMG

text: live

text: Generic

text: Mus musculus

text: 3T3 cells total RNA


text: IMG

text: live

text: Generic

text: Mus musculus

text: mouse lung total RNA


text: IMG

text: live

text: Generic

text: liver

text: Mus musculus

text: liver

text: mouse liver


text: IMG

text: live

text: Generic

text: HeLa

characteristic: cell-line

text: Homo sapiens

text: HeLa cells total RNA


text: IMG

text: live

text: Generic

text: Danio rerio

text: Zebrafish total RNA


text: IMG

text: live

text: Generic

text: Bufotes viridis

text: Bufo total RNA


text: IMG

text: live

text: Generic

text: Gallus gallus

text: Chicken total RNA


text: IMG

text: live

text: Generic

text: Xenopus laevis

text: Xenopus total RNA


text: The University of Georgia, Department of Plant Biology

text: live

text: Generic

text: The library was made from poly-A RNA in the cloning vector lambda ZAP II. Clones to be sequenced were prepared by mass excision.

characteristic: description

text: SOLR strain of E. coli

characteristic: lab-host

text: Sorghum bicolor

text: XhoI

text: EcoRI

text: Dark-grown seedlings

text: Plasmid

text: pBlueScriptSK-


text: live

text: Generic

text: Four-week-old sorghum seedlings were sprayed with spore suspension prepared from 3-week-old FRM42I, a sorghum isolate of the anthracnose pathogen Colletotrichum graminicola. Inoculated plants were kept in a 25 C dark growth chamber with 100% relative humidity for 24 hr, followed by 12/12 hr of light/dark cycle at 25 C with 90% relative humidity for another 24 hr. All leaves were harvested and quick frozen with liquid nitrogen and stored in a -80 C freezer. The library was made from poly-A RNA in the cloning vector lambda ZAP II. Clones to be sequenced were prepared by mass excision. WARNING: While most or all ESTs are expected to derive from the host plant, no effort was made to eliminate ESTs deriving from the pathogen.

text: SOLR strain of E. coli

text: Sorghum bicolor

characteristic: organism

text: XhoI

text: EcoRI

text: Leaves

characteristic: tissue-type

text: Pathogen-induced: compatible

text: Plasmid

text: pBlueScriptSK-


text: live

text: Generic

text: SOLR strain of E. coli

text: Rhizome

text: Sorghum halepense

text: XhoI

text: EcoRI

text: Rhizomes

characteristic: title

text: Plasmid

text: pBlueScriptSK-

characteristic: vector


text: live

text: Generic

text: SOLR strain of E. coli

text: Rhizome

characteristic: organ

text: Sorghum propinquum

text: XhoI

text: EcoRI

text: Sorghum propinquum Rhizomes

text: Plasmid

text: pBlueScriptSK-


characteristic: External Id

text: Hunan Agricultural University

text: live

text: Generic

text: organelle: nucleomorph

text: Sus scrofa

text: pig nucleomorph


text: Stanford University

text: live

text: Generic

text: Library constructed using oligo-dT/NotI primer and cloned into EcoRI/NotI sites. This library consists of unique clones fingerprinted and picked from RZPD library 567. Fingerprinting and arraying by M. Clark (Max Planck Institute, Berlin).

text: embryo

text: XL-1 Blue

text: embryo

text: Danio rerio

text: EcoRI

text: NotI

text: gastrula stage

text: phagemid


text: Maize Research Institute, Sichuan Agricultural University

text: live

text: Generic

text: seedling

text: E. coli DH5alpha

text: Zea mays

text: leaf

text: Maize subtractive cDNA library

text: Plasmid


text: University of Agricultural Sciences

text: live

text: Generic

characteristic: cultivar

text: The subtracted library was constructed from the roots of Finger millet seedlings exposed to severe salt stress of 400 mM NaCl, after prior induction with 200mM NaCl. In the presence of 10uM ABA.

text: E.coli

text: Eleusine coracana

text: EcoRI

text: Seedlings

text: Finger millet salt stress cDNA library

text: TA cloning vector


text: Instituto Tecnologico de Celaya Departamento de Ingenieria Bioquimica

text: live

text: Generic

text: 2 ul PCR fragments were ligated with 1 ul pCR4 -TOPO cloning vector according to the manufacturer's instructions (Invitrogen, Carlsbad, CA, USA). 2 ul ligation reaction solutions were transformed into 50 ul E. coli chemically competent cells strain TOP 10. The transformation culture was plated on Petri dishes containing LB/kamamycin, and the screened colony for insert fragment. Selected colonies containing recombinant plasmid were inoculated separately into 5 ml LB/ampicillin/kanamycin solution and it was shaken at 37 oC overnight. Then 500 ul each culture were added into 2 ml cryogenic vial (Corning, Acton, MA, USA) containing 500 ul 100 % glycerol and kept at -80 oC.

text: 3 days in water

characteristic: dev-stage

text: Allium sativum

text: Blanco perla

characteristic: strain

text: Clove

text: Allium Reverse Subtractive Library

text: Plasmid

text: pCR 4 TOPO TA


characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: 3 days in water

text: Allium sativum

text: Blanco perla

text: Clove

text: Allium Subtractive Library 2

text: Plasmid

text: pCR 4 TOPO TA


text: University of Calgary

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Marianne

text: Treated with Botrytis spp. elicitor for 10 hours

text: Papaver somniferum

text: Cell culture

text: Elicitor-treated opium poppy cell culture Lambda Zap expression library

text: Lambda Zap


text: Centre for Cellular and Molecular Biology

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Robusta coffee Cultivar 'CxR'

text: The library was made using mRNA isolated from moisture-stressed leaf tissue using Clonetch SMART-TM PCR cDNA Synthesis Kit. The cloned fragments were amplified from the recombinant plasmid DNA using M13 universal primers and were sequenced on an automated DNA sequencer ABI3700 using fluorescence based dideoxy terminator chemistry. Moisture-stressed leaf tissue obtained from Department of Plant Physiology, University of Agricultural Sciences, Bangalore, India. Robusta coffee Cultivar 'CxR' (an interspecific hybrid of Coffea congensis X Coffea canephora) developed at Central Coffee Research Institute, Chikamagalur, India

text: Coffea congensis x Coffea canephora

text: moisture-stressed leaf tissue

text: Leaf EST Library of robusta coffee cultivar 'CxR'

text: pMOSBlue Blunt Ended vector


text: National Cancer Institute / NIH

text: live

text: Generic

text: cDNA was primed using oligo-dT primer: GACTAGTTCTAGATCGCGAGCGGCCGCC(T)25 and cloned into the SmaI/NotI sites of pCS111. Size-selection >900 bp resulted in an average insert size of 1.1 kb. This primary library was constructed by Express Genomics (Frederick, MD). Use M13(-21) primer for 3' end reads, and m13rp for 5' end reads. Note: this is a (http://xgc.nci.nih.gov/) Xenopus Gene Collection library.

characteristic: description

text: DH10B TonA

text: limb

text: Xenopus laevis

text: SmaI

text: NotI

text: hind limb

text: plasmid


text: National Cancer Institute / NIH

text: live

text: Generic

text: cDNA was primed using oligo-dT primer: GACTAGTTCTAGATCGCGAGCGGCCGCC(T)25 and cloned into the SmaI/NotI sites of pCS111. Size-selection >900 bp resulted in an average insert size of 1.2 kb. This primary library was constructed by Express Genomics (Frederick, MD). Use M13(-21) primer for 3' end reads and m13rp for 5' reads. Note: this is a (http://xgc.nci.nih.gov/) Xenopus Gene Collection library.

text: DH10B TonA

characteristic: lab-host

text: skin

text: Xenopus laevis

text: SmaI

text: NotI

text: dorsal back skin, pooled

text: plasmid


text: Department of Genetics and Biochemistry, Clemson University

text: live

text: Generic

text: Sweet basil seeds were grown under natural light conditions in the greenhouse. Greenhouse cooling/heating set points were 27/25oC. At the 3rd leaf stage, 0.5 mM MeJA in 2% ethanol was sprayed onto the basil plants. The untreated (control) basil plants were only sprayed with 2% ethanol. Then the MeJA treated and control plants were zipped with a vinyl pack for 40 min. Each of one gram of leaves from the untreated (control) and the MeJA treated sweet basil was randomly sampled at 0, 1, 3, 6, 9, 12, 24, 48 hours. The leaves were immediately stored at -80 oC prior to analysis. Total RNA was extracted respectively from the pooled leaves of the MeJA treated and the control using Trizol method (Invitrogen) according to the manufacturer's recommendations. SSH was performed using a PCR Select(tm) cDNA Subtraction Kit (Clontech) according to the manufacturer's instructions. The subtracted cDNA library was cloned directly into the T/A cloning vector pCR2.1 (Invitrogen). Differential screening was performed using colony array (PCR-Select Differential Screening Kit, Clontech).

text: the 3rd leaf stage (30-40d old seedling)

text: E. coli Top 10F'

text: Ocimum basilicum

characteristic: organism

text: leaves

text: Differential Gene Expression upon Methyl Jasmonate (MeJA) Treatment


text: University of Wuerzburg, Julius-von-Sachs Institute for Biological Sciences

text: live

text: Generic

text: SSH library from extraradical mycelium grown with 100 uM Zn subtracted with extraradical mycelium grown in the presence of 1 uM Zn

text: Daucus carota

text: Rhizophagus intraradices

text: extraradical mycelium

characteristic: tissue-type

text: __giaac Zinc SSH library

text: Plasmid

text: pGEM-T easy


text: live

text: Generic

text: SSH library of mycelium grown asymbiotically in co-culture with Paenibacillus validus subtracted with asymbiotic mycelium grown in the absence of Paenibacillus validus

text: Rhizophagus intraradices

text: asymbiotically grown mycelium

text: __giaad Paenibacillus SSH library

characteristic: title

text: Plasmid

text: pGEM-T easy


text: University of South Carolina

text: live

text: Generic

text: Palaemon pugio

text: male

characteristic: sex

text: Grass shrimp Suppressive-subtractive Hybridization


text: Institute of Biomedical Sciences

text: live

text: Generic

text: sporulating oocysts

text: Eimeria acervulina

text: H

text: ORESTES cDNA library, Eimeria acervulina sporulating oocysts

text: Plasmid

text: pGEM-T Easy

characteristic: vector


characteristic: External Id

text: Institute of Biomedical Sciences

text: live

text: Generic

text: sporulated oocysts

text: Eimeria acervulina

text: H

text: ORESTES cDNA library, Eimeria acervulina sporulated oocysts

text: Plasmid

text: pGEM-T Easy


text: Institute of Biomedical Sciences

text: live

text: Generic

text: unsporulated oocysts

text: Eimeria acervulina

text: H

text: ORESTES cDNA library, Eimeria acervulina unsporulated oocysts

text: Plasmid

text: pGEM-T Easy


text: Institute of Biomedical Sciences

text: live

text: Generic

text: sporozoites

text: Eimeria acervulina

text: H

text: ORESTES cDNA library, Eimeria acervulina sporozoites

text: Plasmid

text: pGEM-T Easy


text: Institute of Biomedical Sciences

text: live

text: Generic

text: sporulating oocysts

text: Eimeria maxima

text: H

text: ORESTES cDNA library, Eimeria maxima sporulating oocysts

text: Plasmid

text: pGEM-T Easy


text: Institute of Biomedical Sciences

text: live

text: Generic

text: sporulated oocysts

text: Eimeria maxima

text: H

text: ORESTES cDNA library, Eimeria maxima sporulated oocysts

text: Plasmid

text: pGEM-T Easy


text: Institute of Biomedical Sciences

text: live

text: Generic

text: unsporulated oocysts

text: Eimeria maxima

text: H

text: ORESTES cDNA library, Eimeria maxima unsporulated oocysts

text: Plasmid

text: pGEM-T Easy


text: Institute of Biomedical Sciences

text: live

text: Generic

text: merozoites

text: Eimeria tenella

text: H

text: ORESTES cDNA library, Eimeria tenella merozoites

text: Plasmid

text: pGEM-T Easy


text: Institute of Biomedical Sciences

text: live

text: Generic

text: sporulating oocysts

text: Eimeria tenella

text: H

text: ORESTES cDNA library, Eimeria tenella sporulating oocysts

text: Plasmid

text: pGEM-T Easy


text: Institute of Biomedical Sciences

text: live

text: Generic

text: sporulated oocysts

text: Eimeria tenella

text: H

text: ORESTES cDNA library, Eimeria tenella sporulated oocysts

text: Plasmid

text: pGEM-T Easy


text: Institute of Biomedical Sciences

text: live

text: Generic

text: unsporulated oocysts

text: Eimeria tenella

text: H

text: ORESTES cDNA library, Eimeria tenella unsporulated oocysts

text: Plasmid

text: pGEM-T Easy


text: Institute of Biomedical Sciences

text: live

text: Generic

text: sporozoites

text: Eimeria tenella

text: H

text: ORESTES cDNA library, Eimeria tenella sporozoites

text: Plasmid

text: pGEM-T Easy


text: University of California

text: live

text: Generic

text: mRNA isolated from whole tissue of sponge with brooded embryos

text: adult and all developmental stages

text: Oscarella pearsei

text: Oscarella pearsei pSport1 Library I

text: Plasmid


text: Heriot-Watt University

text: live

text: Generic

text: Adult female

text: Lepeophtheirus salmonis

text: Whole organism

text: Subtractive hybridization-organophosphate exposure

text: Plasmid

text: pGEM-T Easy


text: Centre for DNA Fingerprinting and Diagnostics

text: live

text: Generic

text: Directional cDNA library was constructed by cloning cDNA fragments into pBluescript II SK(+) vector and sequenced from 5' end

text: fifth instar third day

text: Antheraea mylitta

text: mixed

text: Fat body

text: Antheraea mylitta cDNA library


text: Departement de Biochimie, Universite de Montreal

text: live

text: Generic

text: Spizellomyces punctatus

text: Company (LARGE)


text: live

text: Generic

text: Spizellomyces punctatus

text: Company (SMALL)


text: Nanjing Agricultural University

text: live

text: Generic

text: post-infective juveniles (J2)

text: Mi-resistant tomato cv. Nemarex

text: Meloidogyne arenaria

text: Meloidogyne arenaria forward suppression subtractive hybridization (SSH) library


text: Open Biosystems, Inc.

text: live

text: Generic

text: Tribolium castaneum


text: Open Biosystems, Inc.

text: live

text: Generic

text: Leptinotarsa decemlineata


text: South China Agricultural University

text: live

text: Generic

text: Ascaris suum

text: Ascaris suum male and female cDNA libraries


text: Open Biosystems, Inc.

text: live

text: Generic

text: Danio rerio


text: Open Biosystems, Inc.

text: live

text: Generic

text: dT primed SMART cDNA library

text: Danio rerio

text: embryo day 2 and 3


text: Open Biosystems, Inc.

text: live

text: Generic

text: dT primed SMART cDNA library

text: Danio rerio

text: Day 1 embryo


text: Open Biosystems, Inc.

text: live

text: Generic

text: EST

text: Danio rerio

text: Day 1 embyos

text: pDNR-LIB


text: Open Biosystems, Inc.

text: live

text: Generic

text: EST

text: Danio rerio

text: Day 2 embryos

text: pDNR-LIB


text: live

text: Generic

characteristic: cultivar

text: The library was made using mRNA isolated from moisture-stressed leaf tissue using Clonetch SMART-TM PCR cDNA Synthesis Kit.The cloned fragments were amplified from the recombinant plasmid DNA using M13 universal primers and were sequenced on an automated DNA sequencer ABI3700 using fluorescence based dideoxy terminator chemistry. Coffea arabica 'Selection-12' developed at Central Coffee Research Institute, Chikamagalur, India. Moisture-stressed leaf tissue obtained from Prof. Uday Kumar, Department of Plant physiology, University of Agricultural Sciences, Bangalore, india.

text: Coffea arabica

text: Moisture-stressed leaf tissue

text: Leaf EST library of Coffea arabica cultivar S-12

text: pMOSBlue Blunt Ended vector


text: Sun Yat-sen University School of Life Science

text: live

text: Generic

text: ESTs expressed preferentially in Micronucleate cell of Pseudourostyla cristata during Conjugation phase.

text: Conjugation phase

characteristic: dev-stage

text: Pseudourostyla cristata

text: Pseudourostyla cristata conjugation phase


text: Fundacion Ciencia para La Vida

text: live

text: Generic

text: mycelium

characteristic: cell type

characteristic: cell-line

text: mycelium

text: mycelium

characteristic: organ

text: Botrytis cinerea

text: Xho 1

characteristic: strain

text: mycelium

text: Botrytis cinerea expressed sequence tags

text: plasmid

text: pExpress 1


text: National Institute of Genetics

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Protonemata cultivated on the BCDATG medium were blended by the POLYTRON, and then cultivated on the BCDATG medium at 25C for a week under continuous light. Colonies of protonemata were inoculated on a peat pot and cultivated for 1-1.5 months at 25C under continuous light. Upper part of gametophores with approximately 15 leaves was dissected for RNA extraction. 1st strand cDNA was primed with an oligo (dT) primer [GCGGCTGAAGACGGCCTATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded cDNA was ligated to a DraIII adaptor [GGCCUACUGG], digested and directionally cloned into distinct DraIII sites of the pME18S-FL3. Library was size selected for 1.0 kb, with a average insert size of 1.2kb. Library constructed by Yutaka Suzuki (University of Tokyo, Institute of Medical Science). Custom primers recommended for sequencing: 5' end primer 5'-GGATGTTGCCTTTACTTCTA-3' and 3'end primer 5'-CGACCTGCAGCTCGAGCACA-3'. synonym: Physcomitrella patens subsp. patens

text: gametophores

text: Physcomitrium patens

text: DraIII

text: DraIII

text: Upper half part of gametophores

text: ppls full-length cDNA library


text: The Wellcome Trust Sanger Institute

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: unsporulated oocyst

text: Eimeria tenella

text: Houghton

text: Eimeria tenella Houghton unsporulated oocyst


text: The Wellcome Trust Sanger Institute

text: live

text: Generic

text: first generation merozoite

text: Eimeria tenella

characteristic: organism

text: Wisconsin

text: Eimeria tenella Wisconsin first generation merozoite


text: The Wellcome Trust Sanger Institute

text: live

text: Generic

text: sporozoite

text: Eimeria tenella

text: Houghton

characteristic: title


text: Sabanci University

text: live

text: Generic

text: Agrostis capillaris

text: Leaf

characteristic: tissue-type

text: Colonial bentgrass leaf tissue


text: Sabanci University

text: live

text: Generic

text: Agrostis canina

text: Leaf

text: Velvet bentgrass leaf tissue


text: Sabanci University

text: live

text: Generic

text: Agrostis stolonifera

text: Leaf

text: Creeping bentgrass (Agrostis stolonifera) leaf tissue


text: Open Biosystems, Inc.

text: live

text: Generic

text: Plodia interpunctella


text: Open Biosystems, Inc.

text: live

text: Generic

characteristic: lab-host

text: Danio rerio

characteristic: vector


characteristic: External Id

text: Open Biosystems, Inc.

text: live

text: Generic

text: random primed SMART cDNA library

characteristic: description

text: Danio rerio

text: Day 1 embryo


text: Open Biosystems, Inc.

text: live

text: Generic

text: Danio rerio

text: Day 3 embryos

text: pDNR-LIB


text: Open Biosystems, Inc.

text: live

text: Generic

text: dT primed cDNA

text: Danio rerio

text: Intestine


text: Open Biosystems, Inc.

text: live

text: Generic

text: dT primed cDNA

text: Danio rerio

text: Skin


text: Open Biosystems, Inc.

text: live

text: Generic

text: dT primed cDNA

text: Danio rerio

text: heart


text: Open Biosystems, Inc.

text: live

text: Generic

text: dT primed cDNA

text: Danio rerio

text: gills


text: Open Biosystems, Inc.

text: live

text: Generic

text: dT primed cDNA

text: Danio rerio

text: testis


text: Open Biosystems, Inc.

text: live

text: Generic

text: dT primed cDNA

text: Danio rerio

text: brain


text: Open Biosystems, Inc.

text: live

text: Generic

text: dT primed cDNA

text: Danio rerio

text: eyes


text: Open Biosystems, Inc.

text: live

text: Generic

text: dT primed SMART cDNA library

text: Danio rerio

text: gills

text: pDNR-LIB


text: Open Biosystems, Inc.

text: live

text: Generic

text: dT primed SMART cDNA library

text: Danio rerio

text: testis

text: pDNR-LIB


text: Open Biosystems, Inc.

text: live

text: Generic

text: dT primed SMART cDNA library

text: Danio rerio

text: precursor cells


text: Open Biosystems, Inc.

text: live

text: Generic

text: dT primed cDNA

text: Danio rerio

text: non-stimulated lymphoid


text: Open Biosystems, Inc.

text: live

text: Generic

text: dT primed cDNA

text: Danio rerio

text: bone


text: Open Biosystems, Inc.

text: live

text: Generic

text: dT primed cDNA, >500bp

text: Danio rerio


text: Iwate University

text: live

text: Generic

text: Brassica napus

text: embryonic microspore

text: subtracted cDNA library, embryonic microspore


text: live

text: Generic

text: ESTs expressed preferentially in micronucleate cell of Pseudourostyla cristata during regeneration phase.

text: regeneration phase

characteristic: dev-stage

text: Pseudourostyla cristata

text: Pseudourostyla cristata regeneration phase


text: Open Biosystems, Inc.

text: live

text: Generic

text: Normalized and subtracted KZ41

text: Caenorhabditis elegans


text: Open Biosystems, Inc.

text: live

text: Generic

text: Tribolium castaneum


text: Open Biosystems, Inc.

text: live

text: Generic

text: Caenorhabditis elegans


text: Open Biosystems, Inc.

text: live

text: Generic

text: Drosophila melanogaster


text: Southwest Foundation for Biomedical Research

text: live

text: Generic

text: Forward and reverse subtractive hybridizations were performed with intestinal cDNAs from an opossum with elevated plasma low density lipoprotein cholesterol and intestinal cDNAs from an opossum with normal plasma low density lipoprotein cholesterol using the PCR-Select cDNA subtraction kit (Clontech). The subtracted cDNAs were cloned into the pCR4-TOPO vector (Invitrogen), and the ligation mixtures were transformed into TOP10 cells.

text: adult

text: Monodelphis domestica

text: small intestine

text: Opossum subtracted intestinal cDNA libraries

text: plasmid


text: Universita degli Studi dell'Insubria

text: live

text: Generic

text: Thunnus thynnus

text: ovary

text: T. thynnus ovary cDNA expression library


text: Institute of Animal Science, Chinese Academy of Agriculture Sciences

text: live

text: Generic

text: Sus scrofa

text: Female

characteristic: sex

text: Porcine Oocyte Express Library


text: Institute of Physiology and Biophysic, University of Aarhus

text: live

text: Generic

text: Sequenced PCR product from RT-PCR

text: kidney

characteristic: organ

text: Rattus norvegicus

text: NotI

text: HINDIII

text: male

text: Wistar Kyoto

text: kidney

text: mRNA from rat kidney

text: PCR vector


text: British Columbia Cancer Agency

text: live

text: Generic

text: LNCaP Hollow Fibre Model

characteristic: cell-line

text: Suppression subtractive hybridization performed with hormonal progression of LNCaP Hollow Fibre Model

text: Mouse

text: Homo sapiens

text: LNCaP subtractive hybridization


text: Departement de Biochimie, Universite de Montreal

characteristic: INSDC center name

text: live

text: Generic

text: Acetabularia acetabulum

text: Acetabularia acetabulum Non-normalized


characteristic: INSDC first public

text: live

text: Generic

text: Acanthamoeba castellanii

text: Biotinylated CAP Trapper method


characteristic: INSDC last update

text: live

text: Generic

text: Acanthamoeba castellanii

text: Acanthamoeba castellanii Normalized long fraction


text: live

characteristic: INSDC status

text: Generic

text: Acanthamoeba castellanii

text: Biotinylated CAP Trapper method (without purification of full-length cDNAs)


text: live

text: Generic

characteristic: NCBI submission model

text: Acanthamoeba castellanii

text: Acanthamoeba castellanii Normalized small


text: live

text: Generic

characteristic: NCBI submission package

text: Acanthamoeba castellanii

text: Acanthamoeba castellanii Non-normalized


text: live

text: Generic

text: Acanthamoeba castellanii

characteristic: organism

text: 1:1 combination of non-normalized and normalized-small libraries


text: live

text: Generic

text: Acanthamoeba castellanii

text: Biotinylated CAP Trapper method (Carninci et al)

characteristic: title


text: live

text: Generic

text: Chlamydomonas incerta

text: Chlamydomonas incerta Normalized large


text: live

text: Generic

text: Chlamydomonas incerta

text: Chlamydomonas incerta Normalized small


text: live

text: Generic

text: Chlamydomonas incerta

text: Chlamydomonas incerta Regular


text: live

text: Generic

text: Chlamydomonas incerta

text: Chlamydomonas incerta Regular library 2


text: live

text: Generic

text: Glaucocystis nostochinearum

text: Glaucocystis nostochinearum Normalized


text: live

text: Generic

text: Glaucocystis nostochinearum

text: Normalized (1-4 kb)


text: live

text: Generic

text: Glaucocystis nostochinearum

text: Home made (1.0-2.0)


text: live

text: Generic

text: Glaucocystis nostochinearum

text: Home made (2.0-4.0)


text: live

text: Generic

text: Glaucocystis nostochinearum

text: Home made (0.5-1.0)


text: live

text: Generic

text: Vermamoeba vermiformis

text: Hartmannella vermiformis Regular Small


text: live

text: Generic

text: Vermamoeba vermiformis

text: Hartmannella vermiformis Normalized long fraction


text: live

text: Generic

text: Vermamoeba vermiformis

text: Hartmannella vermiformis Normalized short fraction


text: live

text: Generic

text: Vermamoeba vermiformis

text: Hartmannella vermiformis Regular Large


text: live

text: Generic

text: Isochrysis galbana

text: CCMP 1323

characteristic: strain

text: Isochrysis galbana Normalized long fraction


text: live

text: Generic

text: Isochrysis galbana

text: CCMP 1323

text: Isochrysis galbana Non-normalized long fraction


text: live

text: Generic

text: Karlodinium veneficum

text: Karlodinium micrum Non-normalized


text: live

text: Generic

text: Monosiga ovata

text: Monosiga ovata Non-normalized


text: live

text: Generic

text: Monosiga ovata

text: Monosiga ovata Normalized


text: live

text: Generic

text: Diacronema lutheri

text: Pavlova lutheri Non-normalized


text: live

text: Generic

text: Prototheca wickerhamii

text: Prototheca wickerhamii Normalized small


text: live

text: Generic

text: Prototheca wickerhamii

text: Prototheca wickerhamii Normalized large


text: live

text: Generic

text: Prototheca wickerhamii

text: Prototheca wickerhamii Regular large


text: live

text: Generic

text: Prototheca wickerhamii

text: Prototheca wickerhamii Large regular


text: live

text: Generic

text: Tetradesmus obliquus

text: Scenedesmus obliquus Normalized small


text: live

text: Generic

text: Tetradesmus obliquus

text: Scenedesmus obliquus Regular large


text: live

text: Generic

text: Tetradesmus obliquus

text: Scenedesmus obliquus Normalized large


text: live

text: Generic

text: Tetradesmus obliquus

text: Scenedesmus obliquus Large regular


text: University of British Columbia

text: live

text: Generic

characteristic: cultivar

text: Bark was wounded using razor blades along the entire length of the tree at 5 mm intervals on opposite sides of the trunk. The same trees were also sprayed with a 0.01% (v/v) methyl jasmonate solution resuspended in 0.1% (v/v) tween 20 (~50mLs per tree). Bark tissue with phloem attached was harvested 3 hours, 6 hours and 12 hours after initiating the treatment. Untreated control bark was also harvested at time 0 hours. mRNA was isolated from each tissue source independently and equal quantities of mRNA from each tissue were then pooled. cDNA was prepared from 5 micrograms of mRNA and directionally ligated into the pBluescript II SK (+) XR vector using the pBluescript II XR cDNA Library Construction Kit according to manufacturer's instructions with modifications (Stratagene). Plasmid DNA was then transformed by electroporation into DH10B cells (Invitrogen) for propagation.

text: one year old clonal trees grown under greenhouse conditions in standard potting soil mixture

text: E. coli DH10B cells

characteristic: lab-host

text: Picea engelmannii x Picea glauca

text: EcoRI (5' end of cDNA)

text: XhoI (3' end of cDNA)

text: Hermaphrodite

text: Bark (with phloem and cambium attached)

characteristic: tissue-type

text: pBluescript II SK (+) XR


characteristic: External Id

text: Kazusa DNA Research Institute

text: live

text: Generic

text: Hokuseki

text: three week-old plant

text: Trifolium pratense

text: Trifolium pratense three week-old plant


text: Kazusa DNA Research Institute

text: live

text: Generic

text: Trifolium pratense

text: leaf

text: Trifolium pratense leaf


text: AgResearch Ltd

text: live

text: Generic

text: Approximately equal quantities of RNA from 4 tissues were pooled for cDNA library generation. The tissues were:1. Spleen tissue, 2. Lymph node tissue, 3. Splenocytes stimulated with ConA for 2 hours, 4. Splenocytes stimulated with ConA for 19 hours. A normalised cDNA library was prepared by Clontech from RNA supplied by AgResearch. The cDNA clones were sequenced by TIGR.

characteristic: description

text: Trichosurus vulpecula

text: pooled tissue from Spleen,Lymph node,Splenocytes stimulated with ConA for 2 hours,Splenocytes stimulated with ConA for 19 hours.

text: plasmid

text: PDNRLIB

characteristic: vector


text: AgResearch Ltd

text: live

text: Generic

text: Approximately equal quantities of RNA from epidermal cells scraped from the following 7 regions of the possum gut were pooled for cDNA library generation: The tissues were:1. Duodenum, 2. Ileum, 3. Jejunum, 4. Proximal Colon, 5. Distal Colon, 6. Caecum, 7. Peyers patches. A normalised cDNA library was prepared by Clontech from RNA supplied by AgResearch. The cDNA clones were sequenced by TIGR.

text: Trichosurus vulpecula

text: pooled epidermal cells scraped from Duodenum,Ileum,Jejunum,Proximal Colon,Distal Colon,Caecum,Peyers patches

text: plasmid

text: PDNRLIB


text: Open Biosystems, Inc.

text: live

text: Generic

text: Drosophila melanogaster


text: University of Erlangen

text: live

text: Generic

text: country: Germany:Bavaria

text: Plantago major

text: vascular tissue

text: Plantago major vascular tissue


text: Auburn University

text: live

text: Generic

text: mixed embryonic and adult

characteristic: dev-stage

text: Flaccisagitta enflata

text: Flaccisagitta enflata library


text: Washington University

text: live

text: Generic

text: BD Biosciences 5' RACE kit primed with gene specific primer and 5'CDS, SMARTII A oligo and UPM primers

text: 3 weeks

text: Arabidopsis thaliana

text: Col met1-1

text: seedlings

text: Arabidopsis thaliana Col met1-1 5' RACE


text: Institute of Hydrobiology, Chinese Academy of Sciences

text: live

text: Generic

text: Acipenser sinensis

text: male

characteristic: sex

text: pituitary

text: Chinese sturgeon pituitary cDNA library


text: University of Agricultural Sciences

characteristic: INSDC center name

text: live

text: Generic

text: TMV 2

text: Groundnut 15-day-old seedlings were subjected to different water stress (20%F.C, 40%F.C, 60%F.C and 100%F.C) by withholding water till the crop plants withstands to the respective stress level. RNA isolated from leaf tissues. Subtracted cDNA library was constructed using SMART kit, by modifying it for subtraction and cloned into lambda.

text: 20 day-old seedlings

text: E.coli

text: Arachis hypogaea

text: Sfi I

text: Leaf

text: Groundnut drought stressed subtracted cDNA library

text: Lambda


text: Kobe University Graduate School of Medicine

characteristic: INSDC first public

text: live

text: Generic

text: Mus musculus

text: Brain

text: Mouse brain cap site cDNA


characteristic: INSDC last update

text: live

text: Generic

text: Mus musculus

text: muscle

text: Mouse muscle cap site cDNA


text: Stanford University

text: live

characteristic: INSDC status

text: Generic

text: Directionally cloned. Tissue source: brain tissue from pooled adults of both sexes. Library constructed by J. Ngai.

text: adult

text: GeneHogs DH10B

text: brain/CNS

characteristic: organ

text: Danio rerio

text: SalI

text: NotI

text: both

text: brain

text: Zebrafish adult brain

text: plasmid


text: Brooms Barn Research

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Identified from a Beta vulgaris root cDNA library

text: Polymyxa graminis

text: Polymyxa graminis from Beta vulgaris roots


text: University of Georgia

text: live

text: Generic

text: Cynodon transvaalensis

characteristic: organism

characteristic: title

text: Plasmid


text: Kyungsung University

text: live

text: Generic

text: Total RNA was extracted from tissue, checked for quality, then prepared for construction and normalization of the cDNA library by Invitrogen. Plasmids were isolated and inserts end-sequenced.

text: liver

text: Mugil cephalus

text: cloning vector

text: pGEM-T easy


text: Kyungsung University

text: live

text: Generic

text: Seriola quinqueradiata

text: cloning vector

text: pGEM-T easy


text: The Samuel Roberts Noble Foundation

text: live

text: Generic

text: The N. benthamiana Mixed Elicitor cDNA (cNbME) library was made with N. benthamiana leaf tissue treated with abiotic and biotic elicitors, cloned into a TRV-based vector and transformed into A.tumefaciencs GV2260.

text: Nicotiana benthamiana

text: Gateway

text: Gateway

text: Leaf

text: Plasmid


text: National Institute of Agrobiological Sciences

text: live

text: Generic

text: 5th-instar day-3 larva

text: Bombyx mori

text: mixed

characteristic: strain

text: maxillary galea

text: mxg


text: live

text: Generic

text: cultured cell derived from ovary

text: Bombyx mori

text: ovary

text: BmN


text: Nanjing Agricultural University

text: live

text: Generic

text: Meloidogyne arenaria

text: Meloidogyne arenaria representational difference analysis cDNA library


text: AgResearch Ltd

text: live

text: Generic

text: Approximately equal quantities of RNA from: 1. brain tissue from the hypothalamus region from 3 individuals. 2. whole pituitary glands collected from 18 individuals. A normalised cDNA library was prepared by Clontech from RNA supplied by AgResearch. The cDNA clones were sequenced by TIGR.

text: Trichosurus vulpecula

text: hypothalamus and whole pituitary glands

text: plasmid

text: PDNRLIB


text: AgResearch Ltd

text: live

text: Generic

text: Approximately equal quantities of RNA collected from ovaries at the following 8 stages of development: 1. Late folicular (3 tissue samples). 2. Mid luteal (3 tissue samples). 3. Juvenile (2 tissue samples). 4. Mid pregnancy (2 tissue samples). 5. Early luteal (2 tissue samples). 6. Early/Mid follicular (2 tissue samples). 7. Anaestrous (2 tissue samples). 8. Lactating (2 tissue samples). A normalised cDNA library was prepared by Clontech from RNA supplied by AgResearch. The cDNA clones were sequenced by TIGR.

text: Trichosurus vulpecula

text: ovaries

text: plasmid

text: PDNRLIB


text: AgResearch Ltd

text: live

text: Generic

text: Approximately equal quantities of RNA from the following 8 sources: 1. Whole embryos (2 tissue samples). 2. Whole Joeys 8-11 days old (8 tissue samples). 3. Early male reproductive tract, day 9 to day 57 (13 tissue samples). 4. Early female reproductive tract, day 22 to day 66 (13 tissue samples). 5. Mid male reproductive tract, day 73 to day 113 (10 samples). 6. Mid female reproductive tract, day 78 to day 115 (7 tissue samples). 7. Late male reproductive tract, day 119 to day 168 (7 tissue samples). 8. Late female reproductive tract, day 119 to day 136 (3 tissue samples). A normalised cDNA library was prepared by Clontech from RNA supplied by AgResearch. The cDNA clones were sequenced by TIGR.

text: Trichosurus vulpecula

text: mixed

text: plasmid

text: PDNRLIB


text: AgResearch Ltd

text: live

text: Generic

text: Approximately equal quantities of RNA from the Oviduct, Cul-de-sac and Uterus with tissues collected for these three regions of the reproductive tract collected at 8 different physiological states: Late follicular, Mid luteal, Juvenile, Mid pregnancy, Early luteal, Early/Mid follicular, Anaestrous, Lactating. Each state contributed approximately equal amounts (by weight) of tissue to each of the tissue pools from which each of the 3 RNA preparations was purified. A normalised cDNA library was prepared by Clontech from RNA supplied by AgResearch. The cDNA clones were sequenced by TIGR.

text: Trichosurus vulpecula

text: Oviduct, Cul-de-sac and Uterus

text: plasmid

text: PDNRLIB


text: University of Agricultural Sciences

text: live

text: Generic

text: TMV 2

characteristic: cultivar

text: Groundnut 15-day-old seedlings were subjected to different water stress (20%F.C, 40%F.C, 60%F.C and 100%F.C) by withholding water till the crop plants withstands to the respective stress level. RNA isolated from leaf tissues. Total stress cDNA library was constructed using SMART kit, using RNA from 40-30% FC stressed plants and cloned into lambda.

text: 20 day-old seedlings

text: E.coli

characteristic: lab-host

text: Arachis hypogaea

text: Sfi I

text: Leaf

text: Groundnut drought stress cDNA library

text: Lambda


characteristic: External Id

text: Forestry and Forest Products Research Institute

text: live

text: Generic

text: To obtain mixed-stage nematodes, the nematodes were cultured on fungi (Botrytis cinerea grown on autoclaved barley) for 1 week at 25C and then extracted for 3h at 25C using the Baermann funnel technique. Separated nematodes were washed with 0.1M NaCl. Poly(A)+ RNA was extracted using an RNeasy kit (Qiagen) and subsequently an oligotex-dT30 mRNA purification Kit (Takara). The cDNA library was constructed using the SuperScript Plasmid system (Invitrogen)(vector: pSPORT1; RE_1: SalI; RE_2: NotI). The plasmid construct was transformed into E. coli DH5a by electroporation. Plasmid DNA was isolated and purified using glass fiber filter plates (Millipore).

text: mixed stage

text: E. coli DH5a

text: Bursaphelenchus mucronatus

text: SalI

text: NotI

text: Bursaphelenchus mucronatus mixed-stage (U1) library


text: live

text: Generic

characteristic: description

text: mixed stage

text: E. coli DH5a

text: Bursaphelenchus xylophilus

text: SalI

text: NotI

text: Bursaphelenchus xylophilus mixed-stage library


text: live

text: Generic

text: Nematodes were cultured on the small pine seedling in germ-free environments. Nematodes were separated by Baermann funnel extraction and washed with 0.1M NaCl and 30% sucrose. mRNA was extracted using Micro-FastTrack (Invitrogen). cDNA library was constructed using the SMART PCR cDNA amplification method (Clontech). SMART-amplified cDNAs were directionally ligated into the pDNR-lib vector. Each plasmid construct was transformed into Escherichia coli DH5a by electroporation. Plasmid DNA was isolated and purified using glass fiber filter plates (Millipore).

text: Mixed-stage

text: E. coli DH5a

text: Bursaphelenchus xylophilus

text: Bursaphelenchus xylophilus cDNA library (Kp)

text: pDNR-lib

characteristic: vector


text: live

text: Generic

text: B. xylophilus dauer larvae (dispersal 4th stage larvae) were produced using the artificial pupal chamber method in a pine log as described in (Aikawa and Togashi, 1998). Dauer larvae which migrated into the vector beetles were separated by Baermann funnel extraction (KDi). Dauer larvae which remained in the logs were collected from the wood surface by hand using a platinum wire picker after the logs had been cut into pieces (KDw). Separated nematodes were washed with 0.1M NaCl and 30% sucrose. mRNA was extracted using Micro-FastTrack (Invitrogen). cDNA library was constructed using the SMART PCR cDNA amplification method (Clontech). SMART-amplified cDNAs were directionally ligated into the pDNR-lib vector. Each plasmid construct was transformed into Escherichia coli DH5alpha by electroporation. Plasmid DNA was isolated and purified using glass fiber filter plates (Millipore).

text: Dauer (dispersal 4th stage larvae)

characteristic: dev-stage

text: E. coli DH5a

text: Bursaphelenchus xylophilus

text: Bursaphelenchus xylophilus dauer stage cDNA library

text: pDNR-lib


text: Universita' degli Studi di Torino

text: live

text: Generic

text: A cDNAs library was constructed (using the Clontech PCR-Select(TM) cDNA Subtraction Kit (BD Biosciences) following the manifacture's instruction) subtracting cDNA from Medicago truncatula GFP:HDEL-transformed roots pieces contacted by G. margarita appressoria (tester) with cDNA from the corresponding control roots (driver). Before performing the subtraction the cDNAs from the two RNA populations were PCR amplified using the BD SMART PCR cDNA Synthesis Kit (BD Biosciences) using a limited number of cycles to keep the amplification in the exponential phase.

text: Medicago truncatula

text: roots

characteristic: tissue-type

text: Medicago truncatula Clontech PCR select cDNA subtraction

text: Plasmid

text: pGEM-T


text: University of Agricultural Sciences

characteristic: INSDC center name

text: live

text: Generic

text: TMV 2

text: Groundnut 15-day-old seedlings were subjected to different water stress (20%F.C, 40%F.C, 60%F.C and 100%F.C) by withholding water till the crop plants withstands to the respective stress level. RNA isolated from leaf tissues. Subtracted cDNA library was constructed using Adapter ligation technique and cloned into T/A cloning vector.

text: 20 day-old seedlings

text: E.coli

text: Arachis hypogaea

text: EcoRI and HindIII

text: Leaf

text: Groundnut drought stressed subtracted Adapter ligation cDNA library

text: T/A cloning vector


text: Washington University School of Medicine

characteristic: INSDC first public

text: live

text: Generic

text: Total RNA isolated from S.mediterranea

text: DH10B cells

text: Schmidtea mediterranea

text: NotI

text: EcoRV

text: Agen 0058

text: plasmid


characteristic: INSDC last update

text: live

text: Generic

text: Total RNA isolated from S.mediterranea

text: DH10B cells

text: Schmidtea mediterranea

text: NotI

text: EcoRV

text: plasmid


text: Boyce Thompson Institute for Plant Research

text: live

characteristic: INSDC status

text: Generic

text: winged adult

text: Myzus persicae

text: parthenogenetic female

characteristic: sex

text: tobacco-adapted lineage (red)

text: digestive tract

text: Myzus persicae, tobacco lineage, aphid digestive tract library

text: plasmid

text: pDNR-LIB


text: live

text: Generic

characteristic: NCBI submission model

text: winged adult

text: Myzus persicae

text: parthenogenetic female

text: tobacco-adapted lineage (red)

text: head

text: Myzus persicae, tobacco lineage, aphid head library

text: plasmid

text: pDNR-LIB


text: live

text: Generic

characteristic: NCBI submission package

text: mixed instars

text: Myzus persicae

text: parthenogenetic female

text: tobacco-adapted lineage (red)

text: whole insect

text: Myzus persicae, tobacco lineage raised on Nicotiana benthamiana, whole aphid library

text: plasmid

text: pDNR-LIB


text: live

text: Generic

text: EST library made from Lamprey 11 muscle tissue.

text: Petromyzon marinus

characteristic: organism

text: BsrgI

text: BsrgI

text: Animal11-muscle tissue

text: plasmid


text: Andhra University

text: live

text: Generic

text: Library 1 contains the Transcript derived fragment (TDF) obtained from cDNA-AFLP gels of Beauveria bassiana only expressed when grown on the cuticular extracts of 1) Aphis craccivora 2) Periplaneta americanum separately which were then cloned in pGEM-T vector and sequenced.

text: Beauveria bassiana

text: ITCC 4688

text: Beauveria bassiana ITCC4688 Library 1

characteristic: title

text: pGEM-T (Promega)


text: Andhra University

text: live

text: Generic

text: Library 10 contains the Transcript derived fragment (TDF) obtained from cDNA-AFLP gels of Beauveria bassiana only expressed when grown on the cuticular extracts of 1) Aphis craccivora 2) Spodoptera litura 3) SDY Broth, which were then cloned in pGEM-T vector and sequenced.

text: Beauveria bassiana

text: ITCC 4688

characteristic: strain

text: Beauveria bassiana ITCC4688 Library 10

text: pGEM-T (Promega)


text: Andhra University

text: live

text: Generic

text: Library 11 contains the Transcript derived fragment (TDF) obtained from cDNA-AFLP gels of Beauveria bassiana only expressed when grown on the cuticular extract of Periplaneta americanum, which were then cloned in pGEM-T vector and sequenced.

text: Beauveria bassiana

text: ITCC 4688

text: Beauveria bassiana ITCC4688 Library 11

text: pGEM-T (Promega)


text: Andhra University

text: live

text: Generic

text: Library 12 contains the Transcript derived fragment (TDF) obtained from cDNA-AFLP gels of Beauveria bassiana only expressed when grown on the cuticular extracts of 1) Epilachna vigintioctopunctata 2) Periplaneta americanum, which were then cloned in pGEM-T vector and sequenced.

text: Beauveria bassiana

text: ITCC 4688

text: Beauveria bassiana ITCC4688 Library 12

text: pGEM-T (Promega)


text: Andhra University

text: live

text: Generic

text: Library 2 contains the Transcript derived fragment (TDF) obtained from cDNA-AFLP gels of Beauveria bassiana only expressed when grown on the cuticular extracts of 1) Aphis craccivora 2) Spodoptera litura 3) Periplaneta americanum separately, which were then cloned in pGEM-T vector and sequenced.

text: Beauveria bassiana

text: ITCC 4688

text: Beauveria bassiana ITCC4688 Library 2

text: pGEM-T (Promega)


text: Andhra University

text: live

text: Generic

text: Library 3 contains the Transcript derived fragment (TDF) obtained from cDNA-AFLP gels of Beauveria bassiana only expressed when grown on the cuticular extracts of 1) Aphis craccivora 2) Epilachna vigintioctopunctata 3) Periplaneta americanum separately, which were then cloned in pGEM-T vector and sequenced.

text: Beauveria bassiana

text: ITCC 4688

text: Beauveria bassiana ITCC4688 Library 3

text: pGEM-T (Promega)


text: Andhra University

text: live

text: Generic

text: Library 4 contains the Transcript derived fragment (TDF) obtained from cDNA-AFLP gels of Beauveria bassiana only expressed when grown on the cuticular extracts of 1) Aphis craccivora 2) Spodoptera litura 3) Epilachna vigintioctopunctata 4) Periplaneta americanum separately, which were then cloned in pGEM-T vector and sequenced.

text: Beauveria bassiana

text: ITCC 4688

text: Beauveria bassiana ITCC4688 Library 4

text: pGEM-T (Promega)


text: Andhra University

text: live

text: Generic

text: Library 5 contains the Transcript derived fragment (TDF) obtained from cDNA-AFLP gels of Beauveria bassiana only expressed when grown on the cuticular extract of Aphis craccivora, which were then cloned in pGEM-T vector and sequenced.

text: Beauveria bassiana

text: ITCC 4688

text: Beauveria bassiana ITCC4688 Library 5

text: pGEM-T (Promega)


text: Andhra University

text: live

text: Generic

text: Library 6 contains the Transcript derived fragment (TDF) obtained from cDNA-AFLP gels of Beauveria bassiana only expressed when grown on the cuticular extracts of 1) Aphis craccivora 2) Epilachna vigintioctopunctata, which were then cloned in pGEM-T vector and sequenced.

text: Beauveria bassiana

text: ITCC 4688

text: Beauveria bassiana ITCC4688 Library 6

text: pGEM-T (Promega)


text: Andhra University

text: live

text: Generic

text: Library 7 contains the Transcript derived fragment (TDF) obtained from cDNA-AFLP gels of Beauveria bassiana only expressed when grown on the cuticular extract of Epilachna vigintioctopunctata, which were then cloned in pGEM-T vector and sequenced.

text: Beauveria bassiana

text: ITCC 4688

text: Beauveria bassiana ITCC4688 Library 7

text: pGEM-T (Promega)


text: Andhra University

text: live

text: Generic

text: Library 8 contains the Transcript derived fragment (TDF) obtained from cDNA-AFLP gels of Beauveria bassiana only expressed when grown on the cuticular extracts of 1) Aphis craccivora 2) Spodoptera litura 3) Epilachna vigintioctopunctata, which were then cloned in pGEM-T vector and sequenced.

text: Beauveria bassiana

text: ITCC 4688

text: Beauveria bassiana ITCC4688 Library 8

text: pGEM-T (Promega)


text: Andhra University

text: live

text: Generic

text: Library 9 contains the Transcript derived fragment (TDF) obtained from cDNA-AFLP gels of Beauveria bassiana only expressed when grown on the cuticular extracts of 1) Aphis craccivora 2) Periplaneta americanum 3) SDY Broth, which were then cloned in pGEM-T vector and sequenced.

text: Beauveria bassiana

text: ITCC 4688

text: Beauveria bassiana ITCC4688 Library 9

text: pGEM-T (Promega)


text: University of Liverpool

text: live

text: Generic

text: cDNA library prepared from skin of carp infected with Ichthyophthirius multifiliis, sampled at 3 hours post-infection

text: Adult

text: E.coli Electromax DH10B

text: Cyprinus carpio

text: Sfi1 GGCCATTACGGCC

text: Sfi1 GGCCGCCTCGGCC

text: Skin

text: Carp skin library 1

text: Plasmid


text: University of Liverpool

text: live

text: Generic

text: cDNA library prepared from skin of carp infected with Ichthyophthirius multifiliis, sampled at 72 hours post-infection

text: Adult

text: E.coli Electromax DH10B

text: Cyprinus carpio

text: Sfi1 GGCCATTACGGCC

text: Sfi1 GGCCGCCTCGGCC

text: Skin

text: Carp skin library 2

text: Plasmid


text: Universita degli Studi dell'Insubria

text: live

text: Generic

text: Thunnus thynnus

text: ovary

text: T. thynnus ovary cDNA normalized library


text: Baylor College of Medicine

text: live

text: Generic

text: radial nerve

characteristic: cell type

text: oligo dt priming from poly A+ RNA, directionally cloned

text: E.coli

text: Strongylocentrotus purpuratus

text: NotI

text: MluI

text: adult

text: Sea Urchin Radial Nerve cDNA Library


text: Baylor College of Medicine

text: live

text: Generic

text: Testis

text: E.coli

characteristic: lab-host

text: Strongylocentrotus purpuratus

text: NotI

text: MluI

text: adult

text: Sea Urchin Testis cDNA library


characteristic: External Id

text: Baylor College of Medicine

text: live

text: Generic

text: Coelomocyte

text: E.coli

text: Strongylocentrotus purpuratus

text: NotI

text: MluI

text: adult

text: Sea Urchin Coelomocyte cDNA library


text: Baylor College of Medicine

text: live

text: Generic

text: Gut

characteristic: description

text: E.coli

text: Strongylocentrotus purpuratus

text: NotI

text: MluI

text: adult

text: Sea Urchin Gut cDNA library


text: Baylor College of Medicine

text: live

text: Generic

text: Mesentary

text: E.coli

text: Strongylocentrotus purpuratus

text: NotI

text: MluI

text: adult

text: Sea Urchin Mesentary cDNA library

characteristic: vector


text: Baylor College of Medicine

text: live

text: Generic

text: Lantem

text: E.coli

text: Strongylocentrotus purpuratus

text: NotI

text: MluI

text: adult

text: Sea Urchin Lantem cDNA library


text: Baylor College of Medicine

text: live

text: Generic

text: Tube Foot

text: E.coli

text: Strongylocentrotus purpuratus

text: NotI

text: EcoRV

text: adult

text: Sea Urchin Tube Foot DH10 cDNA library


text: Baylor College of Medicine

text: live

text: Generic

text: Tube Foot

text: E.coli

text: Strongylocentrotus purpuratus

text: Sfi IA

text: Sfi IB

text: adult

text: Sea Urchin Tube Foot DH5 cDNA library

text: pDNR-LIB


text: British Columbia Genome Sciences Centre

text: live

text: Generic

text: LNCaP

characteristic: cell-line

text: LNCaP human prostate cancer cells (American Type Culture Collection; Bethesda, MD) were maintained in RPMI-1640 media (StemCell Technologies; Vancouver, BC) supplemented with 10% fetal bovine serum (FBS; StemCell Technologies) and incubated at 37C with 5% CO2. Cells at passage 38 were plated at a density of approximately 4x10^6 cells per T175 flask. Cells were serum-starved for 48 hours prior to treatment for 16 hours with 10 nM R1881 (PerkinElmer; Woodbridge, Canada). Cells were harvested and total RNA was extracted from the cells using TRIZOL Reagent (Invitrogen Life Technologies, Carlsbad, CA) following the manufacturer's instructions

text: Homo sapiens

text: male

text: prostate

text: LNCAP + R1881 synthetic Androgen human prostate library


text: Uppsala University

text: live

text: Generic

text: Spironucleus barkhanus

text: ATCC 50380

text: Spironucleus Uni-Zap XR EST Library


text: Nagoya University

text: live

text: Generic

text: apple pathotype

text: Alternaria alternata

text: IFO 8984 (= NBRC 8984)

text: Alternaria alternata IFO 8984 (= NBRC 8984)


text: UMR GenHort, INRA

text: live

text: Generic

text: black baccara (r)

characteristic: cultivar

text: Sample name: Apex H. Plant growth place: greenhouse. Harvesting date: 01/10/2003. experimental condition: normal.

text: stage i, ib and ii

characteristic: dev-stage

text: Rosa hybrid cultivar

text: SfiI

text: SfiI

text: apices

characteristic: tissue-type

text: H

text: pDNR-LIB


text: University of Arizona Department of Ecology and Evolutionary Biology

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Clones spotted on the microarray

text: all

text: Drosophila mojavensis

text: female and male

text: San Carlos, Sonora, Mexico

text: all

text: D. mojavensis array

text: Phage


text: Zhejiang University

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: conidium, germ tube and mycelium

text: cucumerinum

text: Fusarium oxysporum f. sp. cucumerinum

text: SalI

text: NotI

text: Asexual conidial life-cycle of Fusarium oxysporum f. sp. cucumerinum

text: plasmid

text: pBS-SK+


text: Sichuan University, China

text: live

text: Generic

characteristic: NCBI submission package

text: Suppression Subtractive Hybridization construct expression library

text: Panax notoginseng

characteristic: organism

text: roots

text: panax notoginseng subtracted cDNA libraries


text: Rubber Research Institute of Malaysia

text: live

text: Generic

text: Total RNA was isolated from latex of field-grown rubber trees. Following preparation of latex mRNA, subsequent steps of cDNA synthesis and library construction were performed using the ZAP-cDNA Synthesis Kit and ZAP-cDNA Gigapack II Gold Cloning Kit (Stratagene). Latex cDNAs cloned in the Lambda Uni-Zap XR vector were amplified once from the packaging reaction. Subsequently, Lambda Uni-Zap XR clones were in vivo excised into pBluescript phagemids for use as sequencing templates.

text: Hevea brasiliensis

text: EcoRI

text: XhoI

text: Latex vessel

characteristic: title

text: Phage

text: Lambda Uni-ZAP XR


text: Washington University

text: live

text: Generic

text: BD Biosciences 5' RACE kit primed with gene specific primer and 5'CDS, SMARTII A oligo and UPM primers

text: 3 weeks

text: Arabidopsis thaliana

text: Col ddm1-1

characteristic: strain

text: seedlings

text: Arabidopsis thaliana Col ddm1-1 5' RACE


text: Harvard Institutes of Medicine

text: live

text: Generic

text: 15-20 weeks of age; 3-4 kg

text: heart

characteristic: organ

text: Oryctolagus cuniculus

text: SalI

text: NotI

text: male

characteristic: sex

text: New Zealand White

text: Rabbit heart cDNA library


characteristic: External Id

text: Departement de Biochimie, Universite de Montreal

text: live

text: Generic

text: Amoebidium parasiticum

text: Phusion polymerase with G/C buffer (lib2_ab_phu_gc)


text: live

text: Generic

text: Amoebidium parasiticum

text: Phusion polymerase with HF buffer (lib3_ab_phu_hf)


text: live

text: Generic

text: Amoebidium parasiticum

text: Advantage polymerase (lib1_ab_adv)


text: live

text: Generic

text: Allomyces macrogynus

text: Regular, Homemade 1-5kb (lib2_am)


text: live

text: Generic

text: Allomyces macrogynus

text: Homemade,Normalized [DSN on inserts from lib_2] (lib3_normalized_amn)


text: live

text: Generic

text: Allomyces macrogynus

text: Allomyces macrogynus Company


text: live

text: Generic

text: Amoeba proteus

text: Pawlowski library


text: live

text: Generic

text: Blastocystis hominis

text: Blastocystis hominis In-House Non-normalized


text: live

text: Generic

text: Blastocystis hominis

text: Blastocystis hominis Regular, non-normalised


text: live

text: Generic

text: Durnford lab

characteristic: description

text: Cyanophora paradoxa

text: Cyanophora paradoxa High light


text: live

text: Generic

text: Durnford lab

text: Cyanophora paradoxa

text: Cyanophora paradoxa Low light regular


text: live

text: Generic

text: Loeffelhardt lab

text: Cyanophora paradoxa

text: Cyanophora paradoxa Low CO2


text: live

text: Generic

text: Loeffelhardt lab

text: Cyanophora paradoxa

text: Cyanophora paradoxa High CO2


text: live

text: Generic

text: Durnford group

text: Euglena gracilis

text: Low light non-normalized long fraction


text: live

text: Generic

text: Durnford group

text: Euglena gracilis

text: Low light non-normalized short fraction


text: live

text: Generic

text: Durnford group

text: Euglena gracilis

text: High light non-normalized short fraction


text: live

text: Generic

text: Durnford group

text: Euglena gracilis

text: High light non-normalized long fraction


text: live

text: Generic

text: Durnford group

text: Euglena gracilis

text: High Light Acclimated


text: live

text: Generic

text: Jakoba bahamiensis

text: Jakoba bahamiensis Home made, regular (1-5 kb)


text: live

text: Generic

text: Jakoba libera

text: Jakoba libera Homemade, Normalized


text: live

text: Generic

text: Jakoba libera

text: Jakoba libera Company (STANDARD)


text: live

text: Generic

text: Mastigamoeba balamuthi

text: Mastigamoeba balamuthi Non-normalized


text: live

text: Generic

text: Mastigamoeba balamuthi

text: Mastigamoeba balamuthi Normalized large


text: live

text: Generic

text: Mastigamoeba balamuthi

text: Mastigamoeba balamuthi Normalized small


text: live

text: Generic

text: Mastigamoeba balamuthi

text: Mastigamoeba balamuthi Normalized Long/Non-normalized 1:1 Mixed Library


text: live

text: Generic

text: Malawimonas californiana

text: Malawimonas californiana Company (LARGE)


text: live

text: Generic

text: Malawimonas californiana

text: Malawimonas californiana Homemade, Normalized (lib5_normalized_mcn)


text: live

text: Generic

text: Malawimonas californiana

text: Malawimonas californiana Company (SMALL)


text: live

text: Generic

text: Malawimonas californiana

text: Malawimonas californiana Home made (NEW)


text: live

text: Generic

text: Malawimonas jakobiformis

text: Malawimonas jakobiformis Home made (1.0-2.0 kb)


text: live

text: Generic

text: Malawimonas jakobiformis

text: Malawimonas jakobiformis Home made (1.0-2.0), normalized


text: live

text: Generic

text: Mesostigma viride

text: Mesostigma viride Regular library 1


text: live

text: Generic

text: Mesostigma viride

text: Mesostigma viride Regular library 2


text: live

text: Generic

text: Nephroselmis olivacea

text: Nephroselmis olivacea Regular


text: live

text: Generic

text: Capsaspora owczarzaki

text: Capsaspora owczarzaki Amplicon express


text: live

text: Generic

text: Polytomella parva

text: PD(dark)


text: live

text: Generic

text: Polytomella parva

text: PL(light)


text: live

text: Generic

text: Heterostelium pallidum


text: Kobe University

text: live

text: Generic

text: Coturnix japonica

text: female

text: brain

text: Coturnix japonica brain


text: Ibba, CNR

text: live

text: Generic

text: vegetative hyphae

text: Tuber borchii

text: mycelium

text: Tuber borchii mycelium vegetative hyphae


text: National Institute of Genetics

text: live

text: Generic

text: varied

text: Haplochromis chilotes

text: jaw

text: HCEST library


text: National Institute of Genetics

text: live

text: Generic

text: varied

text: Haplochromis sp. 'redtail sheller'

text: jaw

text: HREST library


text: University of Bath

text: live

text: Generic

text: Priapulus caudatus

text: Priapulus caudatus cDNA library


text: Institute of Biology - Amazonas Federal University

text: live

text: Generic

text: fruits containing seeds from various maturation stages

text: Paullinia cupana var. sorbilis

text: BRS-Amazonas

text: Guarana fruits cDNA library

characteristic: vector


text: Korea University College of Medicine

text: live

text: Generic

text: mRNA from normal rat brain on postnatal day 0

text: postnatal day 0

text: Rattus norvegicus

text: male

text: Sprague-Dawley

text: brain

text: normal rat brain on postnatal day 0


text: Pastoral Genomics, c/o ViaLactia Biosciences (NZ) Ltd

text: live

text: Generic

text: Bronsyn

characteristic: cultivar

text: 10 days.

text: flower

text: Lolium perenne

text: spike

text: Perennial ryegrass spike 10 days

text: pBluescriptIISK+


text: live

text: Generic

text: Bronsyn

text: flag stage.

text: flower

text: Lolium perenne

text: spike

text: Perennial ryegrass spike flag stage

text: pBluescriptIISK+


text: Departement de Biochimie, Universite de Montreal

text: live

text: Generic

text: Reclinomonas americana

text: Reclinomonas americana Company (REGULAR)


text: live

text: Generic

text: Reclinomonas americana

text: Reclinomonas americana Company (LARGE)


text: live

text: Generic

text: Reclinomonas americana

text: Reclinomonas americana Home made (NEW)


text: live

text: Generic

text: Reclinomonas americana

text: Reclinomonas americana Home made, Dec. 2003 (1-2kb)


text: live

text: Generic

text: Reclinomonas americana

text: Reclinomonas americana Home made, Dec. 2003 (2-5kb)


text: live

text: Generic

text: Reclinomonas americana

text: Reclinomonas americana Home made, normalized (normalized_ran)


text: live

text: Generic

text: Reclinomonas americana

text: Reclinomonas americana Homemade, Normalized (normalized_pBFL1cat_ran)


text: live

text: Generic

text: Reclinomonas americana

text: Reclinomonas americana Home made (OLD)


text: live

text: Generic

text: Sphaeroforma arctica


text: live

text: Generic

text: Saitoella complicata

text: Advantage polymerase (lib1_sc_adv)


text: live

text: Generic

text: Seculamonas ecuadoriensis

text: Seculamonas ecuadoriensis Company (STANDARD)


text: live

text: Generic

text: Taphrina deformans

text: Phusion polymerase with G/C buffer (lib2_td_gc)


text: live

text: Generic

text: Taphrina deformans

text: Advantage polymerase (lib1_td_adv)


text: live

text: Generic

text: Paratrimastix pyriformis

text: Trimastix pyriformis Non-normalized


text: live

text: Generic

text: Paratrimastix pyriformis

text: Trimastix pyriformis Normalized large


text: live

text: Generic

text: Paratrimastix pyriformis

text: Trimastix pyriformis Normalized small


text: live

text: Generic

text: Paratrimastix pyriformis

text: Trimastix pyriformis Non normalized (Agincourt)


text: live

text: Generic

text: Diplonema papillatum

text: Diplonema papillatum Home made nuclear(1.0-2.0)


text: live

text: Generic

text: Diplonema papillatum

text: Homemade, Regular (lib2_dp)


text: live

text: Generic

text: Diplonema papillatum

text: Homemade, normalized_dpn


text: live

text: Generic

text: Micromonas pusilla

text: library #1


text: live

text: Generic

text: Histiona aroides

text: Home-made, regular (lib1_ha)


text: live

text: Generic

text: Didymium dachnayum

text: Hyperamoeba dachnaya Non-normalized (Amplicon Express)


text: Beijing Engineering Research Center for Hybrid Wheat

text: live

text: Generic

text: Triticum aestivum

text: Spikelet

text: Wheat Spikelet


text: Plant Research International

text: live

text: Generic

text: Separate cDNA libraries were constructed from developing core fibers, developing bast fibers, near mature bast fibers and flower bracts

text: Cannabis sativa

text: Core tissue, bast tissue, flower bracts

text: Hemp Uni-ZAP XR cDNA library

text: Plasmid

text: pBluescript SK


text: Guangxi University; Zhejiang University

text: live

text: Generic

text: twelve-leaf-old (i.e. at the stage of pistil and stamen differentiation) maize plants grown under normal conditions and drought-, salt-, and alkali co-stress for 48h.

text: twelve-leaf-old plants grown under normal conditions and drought-, salt-, and alkali co-stress for 48h

characteristic: dev-stage

text: Zea mays

text: mix tissues (leaf, stem, floral bud)

text: Normalized cDNA library from mix tissues of maize

text: pBluescript SK(+)


text: The University of Arizona

text: live

text: Generic

text: Maize Full length cDNA library constructed by Invitrogen from multiple tissues: 7 day seedling, 7 day seedling with 2 day salt treatment, 4 hour 45 C heat shock, 24 hour 10 C treatment, 4 hour UV-B treatment, 2 day osmotic stress, 7 day etiolated seedling and Juvenile leaf. Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; This is a Gateway compatible vector, permitting clone movement to new vector backbones for expression in diverse host cells using recombination rather than restriction enzymes. poly(A)+ mRNA was prepared by Invitrogen, and equimolar amounts of RNA from each tissue samples were mixed together for selection of mRNA with a 5' cap. After synthesis of cDNA, a normalization step was conducted against the mixture of RNA sources.

text: mixed

text: DH10B T1 phage resistant

characteristic: lab-host

text: Zea mays

text: EcoRI

text: NotI

text: mixed

characteristic: tissue-type

text: Plasmid


text: University of Florida

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: Amplified cDNA library. Library split by size >1.5 KB (XBT_L) and size <1.5KB (XBT_S)

text: Xenoturbella bocki

text: whole animal

text: Xenoturbella bocki, whole animal expression library (XBT_L)

text: plasmid

text: pGemT


text: University of Florida

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Xenoturbella bocki

text: whole animal

text: Xenoturbella bocki, whole animal expression library (XBT_S)

text: plasmid

text: pGemT


text: ARC Seibersdorf research GmbH

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Embryonic

characteristic: cell type

text: E2-24-T embryonic cell suspension line

characteristic: cell-line

text: From SSH library of embryonic cell suspension (wheat line E2-24-T, resistant to Fusarium head blight caused by Fusarium graminearum) following treatment with Fusarium graminearum culture filtrate. Compare also Buerstmayr, H., Lemmens, M., Hartl, L. Doldi, L., Steiner, B., Stierschneider, M., Ruckenbauer, P. (2002) Molecular mapping of QTLs for Fusarium head blight resistance in spring wheat. I. Resistance to fungal spread (Type II resistance). Theoretical and Applied Genetics, 104, 84-91.

text: immature grain, 10 hr post inoculation

text: Flower

text: Triticum aestivum

text: Embryo

text: Wheat-Fusarium graminearum filtrate interaction


text: Institute of Applied Science and Technology (ICAT)

text: live

text: Generic

text: Regent and Trincadeira

text: The cDNA library was constructed through a combination of the SMART library construction and the Gateway method. cDNA sequences were clones into pDNORTM221 Gateway vector.

text: E.coli

text: Leaf

characteristic: organ

text: Vitis vinifera

characteristic: organism

text: Leaf

text: Vitis vinifera Regent and Trincadeira SMART-GATEWAY combined cDNA library

characteristic: title

text: plasmid


characteristic: External Id

text: Pontificia Universidad Catolica de Chile

text: live

text: Generic

text: cDNA-AFLP was carrying out to identify genes that expressed during early stages of somatic embryogenesis in Pinus radiata. The expression was compared with non-embryogenic cells

characteristic: description

text: Early embryo

text: Pinus radiata

text: Embryogenic tissue

text: Early Somatic embryo cDNA-AFLP


text: Sabanci University

text: live

text: Generic

text: Triticum turgidum subsp. durum

text: durum

characteristic: sub species

text: Leaf

text: Durum wheat leaf tissue


text: NewBioTechnic, S.A. (NBT)

text: live

text: Generic

text: Trichoderma lixii

text: CECT 2413

characteristic: strain

text: mycelium Lambda Zap Library L02


text: NewBioTechnic, S.A. (NBT)

text: live

text: Generic

text: Trichoderma lixii

text: CECT 2413

text: mycelium Lambda Zap Library L11


text: NewBioTechnic, S.A. (NBT)

text: live

text: Generic

text: Trichoderma lixii

text: CECT 2413

text: mycelium Lambda Zap Library L15


text: live

text: Generic

text: Trichoderma lixii

text: CECT 2413

text: mycelium Lambda Zap Library L16


text: live

text: Generic

text: Trichoderma lixii

text: CECT 2413

text: mycelium Lambda Zap Library L91


text: live

text: Generic

text: Trichoderma lixii

text: CECT 2413

text: mycelium Lambda Zap Library L17


text: NewBioTechnic, S.A. (NBT)

text: live

text: Generic

text: Trichoderma lixii

text: CECT 2413

text: mycelium Lambda Zap Library L03


text: NewBioTechnic, S.A. (NBT)

text: live

text: Generic

text: Trichoderma lixii

text: CECT 2413

text: mycelium Lambda Zap Library L05


text: NewBioTechnic, S.A. (NBT)

text: live

text: Generic

text: Trichoderma lixii

text: CECT 2413

text: mycelium Lambda Zap Library L06


text: NewBioTechnic, S.A. (NBT)

text: live

text: Generic

text: Trichoderma lixii

text: CECT 2413

text: mycelium Lambda Zap Library L08


text: NewBioTechnic, S.A. (NBT)

text: live

text: Generic

text: Trichoderma lixii

text: CECT 2413

text: mycelium Lambda Zap Library L10


text: The Institutes for Applied Research, Ben Gurion University

text: live

text: Generic

text: Cistus incanus

text: Cistus incanus Differentially expressed cDNA-AFLP fragments


text: NRC Institute for Marine Biosciences Industrial Partnership Facility

text: live

text: Generic

text: Gadus morhua

text: gill

text: Gadus morhua gill library gmnbgi


text: live

text: Generic

text: Gadus morhua

text: brain

text: Gadus morhua brain library gmnbbr


text: New Mexico State University

text: live

text: Generic

text: drought-stressed

characteristic: dev-stage

text: Root

text: Phaseolus acutifolius

text: Xho 1

text: Tepary bean root cDNA

text: Phage

text: Zap

characteristic: vector


text: National Chung Hsing University

characteristic: INSDC center name

text: live

text: Generic

text: Chicken Shell Gland cDNA Library, SuperScript Plasmid System with Gateway Technology for cDNA Synthesis and Cloning, Invitrogen (Cat. #18248-013 and #18248-039)

text: Adult (56 weeks of age)

text: DH10B (Invitrogen)

characteristic: lab-host

text: Gallus gallus

text: SalI

text: NotI

text: Female

characteristic: sex

text: Taiwan Country Chicken L2 Strain

text: Shell Gland

characteristic: tissue-type

text: Chicken Shell Gland cDNA Library

text: pCMV_SPORT6 (Invitrogen)


text: National Chung Hsing University

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Chicken Ovarian Follicles cDNA Library, SuperScript Plasmid System with Gateway Technology for cDNA Synthesis and Cloning, Invitrogen (Cat. #18248-013 and #18248-039)

text: Adult (56 weeks of age)

text: DH10B (Invitrogen)

text: Gallus gallus

text: SalI

text: NotI

text: Female

text: Taiwan Country Chicken B and L2 Strain (Mix)

text: Ovarian Follicles

text: Chicken Ovarian Follicles cDNA Library

text: pCMV_SPORT6 (Invitrogen)


text: ICBIBE, Universitat de Valencia

text: live

text: Generic

characteristic: NCBI submission package

text: Suppression Subtractive Hybridization library using cDNA derived from adult aphids reared at short day conditions (10L:14D) as tester and cDNA derived from adult aphids reared at long day conditions (16L:8D) as driver. The name of the library stands for Short Day Upregulated genes (SDU).

text: adults

text: Acyrthosiphon pisum

text: parthenogenetic females

text: whole insect

text: plasmid

text: pGEM-T Easy


text: ICBIBE, Universitat de Valencia

text: live

text: Generic

text: Suppression Subtractive Hybridization library using cDNA derived from adult aphids reared at long day conditions (16L:8D) as tester and cDNA derived from adult aphids reared at short day conditions (10L:14D) as driver. The name of the library stands for Short Day Downregulated genes (SDD).

text: adults

text: Acyrthosiphon pisum

characteristic: organism

text: parthenogenetic females

text: whole insect

text: plasmid

text: pGEM-T Easy


text: Universita degli Studi dell'Insubria

text: live

text: Generic

text: adult

text: Thunnus thynnus

text: testis

text: T. thynnus adult testis cDNA expression library

characteristic: title


characteristic: External Id

text: Freie Universitaet Berlin

text: live

text: Generic

text: country: Germany:Berlin

text: 28 day old

text: Sus scrofa

text: jejunum

text: jejunum of a 28 day old pig


text: University of British Columbia

text: live

text: Generic

text: Cabernet Sauvignon

characteristic: cultivar

text: WIN01 is a cDNA library made from pericarp tissue collected from 8 stages of berry development, spanning from fruit set to full maturity. Tissue was collected from mature field grown plants, clone 15 grafted on rootstock 101-14. The library was constructed using Stratagene's Zap Express cDNA Gigapack III Gold Cloning Kit.

characteristic: description

text: Fruit set to maturity

text: E.coli XLOLR

text: Vitis vinifera

text: XhoI

text: EcoRI

text: Hermaphrodite

text: Pericarp

text: Cab Sauv pericarp non-normalized (WIN01)

text: Phagemid

text: pBK-CMV SK+


text: University of British Columbia

text: live

text: Generic

text: Cabernet Sauvignon

text: WIN02 is a cDNA library made from a combination of flower, leaf and root tissue. Tissue was collected from mature field grown plants, clone 15 grafted on rootstock 101-14. Flowers were harvested at pre-anthesis development stage, leaves were fully expanded when harvested and roots were produced by air-layering (most were 1-2 mm in diameter). The library was constructed using Stratagene's Zap Express cDNA Gigapack III Gold Cloning Kit.

text: Flower, pre-anthesis; leaf, fully expanded; root, produced by air-layering

text: E.coli XLOLR

text: Vitis vinifera

text: XhoI

text: EcoRI

text: Hermaphrodite

text: Flower, leaf and root

text: Cab Sauv flower, leaf and root non-normalized (WIN02)

text: Phagemid

text: pBK-CMV SK+


text: University of British Columbia

text: live

text: Generic

text: Cabernet Sauvignon

text: WIN03 is a cDNA library made from seed tissue collected from 8 stages of berry development, spanning from fruit set to full maturity. Tissue was collected from mature field grown plants, clone 15 grafted on rootstock 101-14. The library was constructed using Stratagene's cDNA Synthesis Kit and was subtracted to Cot 5 following a protocol adapted from Bonaldo et al. 'Normalization and subtraction: Two approaches to facilitate gene discovery' (1996) Genome Research 6: 791-806.

text: Fruit set to maturity

text: E.coli DH10B

text: Vitis vinifera

text: XhoI

text: EcoRI

text: Hermaphrodite

text: Seed

text: Cab Sauv seed normalized (WIN03)

text: Plasmid

text: pBluescript II SK+


text: University of British Columbia

text: live

text: Generic

text: Cabernet Sauvignon

text: WIN04 is a cDNA library made from pericarp tissue collected from 8 stages of berry development, spanning from fruit set to full maturity. Tissue was collected from mature field grown plants, clone 15 grafted on rootstock 101-14. The library was constructed using Stratagene's cDNA Synthesis Kit and was subtracted to Cot 5 following a protocol adapted from Bonaldo et al. 'Normalization and subtraction: Two approaches to facilitate gene discovery' (1996) Genome Research 6: 791-806.

text: Fruit set to maturity

text: E.coli DH10B

text: Vitis vinifera

text: XhoI

text: EcoRI

text: Hermaphrodite

text: Pericarp

text: Cab Sauv pericarp normalized (WIN04)

text: Plasmid

text: pBluescript II SK+


text: University of British Columbia

text: live

text: Generic

text: Cabernet Sauvignon

text: WIN05 is a cDNA library made from a combination of flower, leaf and root tissue. Tissue was collected from mature field grown plants, clone 15 grafted on rootstock 101-14. Flowers were harvested at pre-anthesis development stage, leaves were fully expanded when harvested and roots were produced by air-layering (most were 1-2 mm in diameter). The library was constructed using Stratagene's cDNA Synthesis Kit and was subtracted to Cot 5 following a protocol adapted from Bonaldo et al. 'Normalization and subtraction: Two approaches to facilitate gene discovery' (1996) Genome Research 6: 791-806.

text: E.coli DH10B

text: Vitis vinifera

text: XhoI

text: EcoRI

text: Hermaphrodite

text: Flower, leaf and root

text: Cab Sauv flower, leaf and root normalized (WIN05)

text: Plasmid

text: pBluescript II SK+


text: University of British Columbia

text: live

text: Generic

text: Cabernet Sauvignon

text: WIN06 is a cDNA library made from seed tissue collected from 8 stages of berry development, spanning from fruit set to full maturity. Tissue was collected from mature field grown plants, clone 15 grafted on rootstock 101-14. The library was constructed using Stratagene's cDNA Synthesis Kit and was subtracted to Cot 7 following a protocol adapted from Bonaldo et al. 'Normalization and subtraction: Two approaches to facilitate gene discovery' (1996) Genome Research 6: 791-806.

text: Fruit set to maturity

text: E.coli DH10B

text: Vitis vinifera

text: XhoI

text: EcoRI

text: Hermaphrodite

text: Seed

text: Cab Sauv seed normalized (WIN06)

text: Plasmid

text: pBluescript II SK+


text: University of British Columbia

text: live

text: Generic

text: Cabernet Sauvignon

text: WIN07 is a cDNA library made from pericarp tissue collected from 8 stages of berry development, spanning from fruit set to full maturity. Tissue was collected from mature field grown plants, clone 15 grafted on rootstock 101-14. The library was constructed using Stratagene's cDNA Synthesis Kit and was subtracted to Cot 7 following a protocol adapted from Bonaldo et al. 'Normalization and subtraction: Two approaches to facilitate gene discovery' (1996) Genome Research 6: 791-806.

text: Fruit set to maturity

text: E.coli DH10B

text: Vitis vinifera

text: XhoI

text: EcoRI

text: Hermaphrodite

text: Pericarp

text: Cab Sauv pericarp normalized (WIN07)

text: Plasmid

text: pBluescript II SK+


text: University of British Columbia

text: live

text: Generic

text: Cabernet Sauvignon

text: WIN08 is a cDNA library made from seed tissue collected from 8 stages of berry development, spanning from fruit set to full maturity. Tissue was collected from mature field grown plants, clone 15 grafted on rootstock 101-14. The library was constructed using Stratagene's cDNA Synthesis Kit and was subtracted to Cot 5 with additional subtraction against the 4 most abundant genes (2S albumin, dehydrin, a glycoprotein and a proline rich hypothetical protein) following a protocol adapted from Bonaldo et al. 'Normalization and subtraction: Two approaches to facilitate gene discovery' (1996) Genome Research 6: 791-806.

text: Fruit set to maturity

text: E.coli DH10B

text: Vitis vinifera

text: XhoI

text: EcoRI

text: Hermaphrodite

text: Seed

text: Cab Sauv seed normalized (WIN08)

text: Plasmid

text: pBluescript II SK+


text: University of British Columbia

text: live

text: Generic

text: Cabernet Sauvignon

text: WIN09 is a cDNA library made from pericarp tissue collected from 8 stages of berry development, spanning from fruit set to full maturity. Tissue was collected from mature field grown plants, clone 15 grafted on rootstock 101-14. The library was constructed using Stratagene's cDNA Synthesis Kit and was subtracted to Cot 2.5 following a protocol adapted from Bonaldo et al. 'Normalization and subtraction: Two approaches to facilitate gene discovery' (1996) Genome Research 6: 791-806.

text: Fruit set to maturity

text: E.coli DH10B

text: Vitis vinifera

text: XhoI

text: EcoRI

text: Hermaphrodite

text: Pericarp

text: Cab Sauv pericarp normalized (WIN09)

text: Plasmid

text: pBluescript II SK+


text: Centro Nacional de Biotecnologia, CSIC

text: live

text: Generic

text: Muscat Hamburg

text: WIN10 is a cDNA library made from pericarp tissue collected from 5 stages of berry development, spanning from 50% veraison to full maturity (>150 g/L NaCl). Tissue was collected from mature field grown plants. The library was constructed using Stratagene's cDNA Synthesis Kit and was normalized to Cot 5 following a protocol adapted from Bonaldo et al. 'Normalization and subtraction: Two approaches to facilitate gene discovery' (1996) Genome Research 6: 791-806.

text: Fruit set to maturity

text: E.coli DH10B

text: Vitis vinifera

text: XhoI

text: EcoRI

text: Hermaphrodite

text: Pericarp

text: Muscat Hamburg post-veraison pericarp normalized (WIN10)

text: Plasmid

text: pBluescript II SK+


text: Centro Nacional de Biotecnologia, CSIC

text: live

text: Generic

text: Muscat Hamburg

text: WIN11 is a cDNA library made from pericarp tissue collected from anthesis flower and 4 stages of berry development, spanning from fruit set (2mm) to just prior to veraison (10 mm). Tissue was collected from mature field grown plants. The library was constructed using Stratagene's cDNA Synthesis Kit and was normalized to Cot 5 following a protocol adapted from Bonaldo et al. 'Normalization and subtraction: Two approaches to facilitate gene discovery' (1996) Genome Research 6: 791-806.

text: Anthesis flower to prior to veraison

text: E.coli DH10B

text: Vitis vinifera

text: XhoI

text: EcoRI

text: Hermaphrodite

text: Berry

text: Muscat Hamburg pre-veraison berry normalized (WIN11)

text: Plasmid

text: pBluescript II SK+


text: Institute of Genetics and Developmental Biology, CAS

text: live

text: Generic

text: The alien additional chromosome of Tai-27 was microdissected by micro-glassneedle under microscopy. Two rounds LA-PCR were carried out with the alien additional chromosome after digesting by proteinase K. The ESTs of the alien additional chromosome of Tai-27 were separated by using Hybrid Specific Amplification (HSA).

text: Triticum aestivum/Thinopyrum intermedium alien addition line

text: PstI

text: PstI

characteristic: strain

text: The Hybrid Specific Amplification Lib of the Wheat-Thinopyrum alien additional line Tai-27

text: PUCmT-Vecter


text: Agricultural Institute of Slovenia

text: live

text: Generic

text: A differential display RT-PCR method was used to identify differentially expressed transcripts in leaves of control and drought-stressed plants.

text: leaf

characteristic: organ

text: Phaseolus vulgaris

text: drought stress in common bean


text: Max-Planck-Institute for Molecular Genetics

text: live

text: Generic

text: Non-normalized cDNA library of sheep tibia fracture callus tissue. The library was constructed with pooled polyA RNA from one animal at 2 weeks, 3 weeks and 4.5 weeks postfracture stages. It was cloned into pDONR222 using Gateway-Cloning. The template for sequencing was prepared by PCR amplification of cDNA inserts with M13 primers.

text: E. coli DH10B-T1r

text: bone

text: Ovis aries

text: female

text: healing fracture callus

text: adult sheep fracture callus pool

text: Plasmid

characteristic: vector


characteristic: INSDC center name

text: live

text: Generic

text: Non-normalized cDNA library of sheep tibia fracture callus tissue. Library was constructed with polyA RNA from fracture callus from 4 animals at postfracture day 7. It was cloned into pDONR222 using Gateway-Cloning. The template for sequencing was prepared by automated plasmid purification.

text: E. coli DH10B-T1r

text: bone

text: Ovis aries

text: female

text: healing fracture callus

text: adult sheep fracture callus 7d

text: Plasmid


text: live

text: Generic

text: Non-normalized cDNA library of sheep tibia fracture callus tissue. Library was constructed with polyA RNA from fracture callus from 3 animals at postfracture day 10. It was cloned into pDONR222 using Gateway-Cloning. The template for sequencing was prepared by automated plasmid purification.

text: E. coli DH10B-T1r

characteristic: lab-host

text: bone

text: Ovis aries

text: female

text: healing fracture callus

text: adult sheep fracture callus 10d

text: Plasmid


text: Northeast Forestry University

text: live

text: Generic

text: Puccinellia tenuiflora

text: LEAF

text: cDNA Library of Puccinellia tenuiflora


text: Departement de Biochimie, Universite de Montreal

text: live

text: Generic

text: Rhizopus arrhizus

text: Rhizopus oryzae Company


text: live

text: Generic

text: Rhizopus arrhizus

text: Rhizopus oryzae Home made (1-5 kb) Glucose


text: live

text: Generic

text: Rhizopus arrhizus

text: Rhizopus oryzae Home made (1-5 kb) Glycerol


text: live

text: Generic

text: Total RNA was extracted from tissue, checked for quality, then prepared for construction and normalization of the cDNA library by Invitrogen. Plasmids were isolated and inserts end-sequenced by the Marine DNA Sequencing and Analysis Facility at the Center for Marine Functional Genomic Studies, Mount Desert Island Biological Laboratory (J.N.Forrest, Jr., Director). Traces were processed for submission to dbEST by trace2dbest software (Parkinson, Anthony and Blaxter, unpublished software). The project was supported by NIH Grant Number P20-RR-16463 from the INBRE Program of the National Center for Research Resources (P.H.Hand, PI).

text: Adult

characteristic: dev-stage

text: Squalus acanthias

text: Rectal gland

text: Dogfish Shark Rectal Gland, Normalized

text: pCMV Sport 6.1


text: Kyorin University

text: live

text: Generic

text: Drosophila ananassae

text: Drosophila ananassae library (Sato H)


text: CNRS/EPHE/UPVD

text: live

text: Generic

text: whole body

characteristic: cell type

text: mRNA was harvested from snail susceptible and resistant towards Echinostoma caproni. Combined cDNAs from the two snail strains were subtracted using the PCR-Select Suppression Subtractive Hybridization kit (SSH) (Clontech, Palo Alto, CA). The resulting library was cloned into pCR4(r)-TOPO(r) (Invitrogen, Carlsbad, CA) and sequenced using the forward plasmid-specific primer T7.

text: Biomphalaria glabrata

text: hermaphrodite

characteristic: sex

text: Bg.CB

text: BgCBWB

text: Plasmid

text: pCR4(r)-TOPO(r) (Invitrogen)


text: CNRS/EPHE/UPVD

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: whole body

text: mRNA was harvested from snail resistant and susceptible towards Echinostoma caproni. Combined cDNAs from the two snail strains were subtracted using the PCR-Select Suppression Subtractive Hybridization kit (SSH) (Clontech, Palo Alto, CA). The resulting library was cloned into pCR4(r)-TOPO(r) (Invitrogen, Carlsbad, CA) and sequenced using the forward plasmid-specific primer T7.

text: Biomphalaria glabrata

text: hermaphrodite

text: Bg.EAF

text: BgEAFWB

text: Plasmid

text: pCR4(r)-TOPO(r) (Invitrogen)


text: University of Southern Denmark

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Roche 5' RACE kit primed with gene specific primer and oligo dT primer. EST sequences were cloned into pCR2.1-TOPO vector using the Zero Blunt TOPO PCR Cloning Kit (Invitrogen).

text: third instar larvae

text: Drosophila melanogaster

text: Canton S

text: Drosophila melanogaster Canton S 5' RACE


text: Rockefeller University

text: live

text: Generic

characteristic: NCBI submission package

text: Taeniopygia guttata

characteristic: organism

text: Embryonic library


characteristic: External Id

text: Rockefeller University

text: live

text: Generic

text: Taeniopygia guttata

text: Developmental library I

characteristic: title


text: Rockefeller University

text: live

text: Generic

text: Taeniopygia guttata

text: Developmental library II


text: Rockefeller University

text: live

text: Generic

text: Taeniopygia guttata

text: Developmental library III


text: Boyce Thompson Institute for Plant Research

text: live

text: Generic

text: winged adult

text: Myzus persicae

text: male

text: Geneva 006

text: whole insect

characteristic: tissue-type

text: Myzus persicae, line G006, whole aphid library

text: plasmid

text: pDNR-LIB


text: Institute for Evolution and Biodiversity

text: live

text: Generic

text: country: Germany:Baltic Sea

characteristic: description

text: Zostera marina

text: leaf

text: EST library A and C


text: Universidad Tecnica Federico Santa Maria

text: live

text: Generic

text: Thompson-seedless

characteristic: cultivar

text: Fruit

characteristic: organ

text: Vitis vinifera

text: BsrG I

text: BsrG I

text: Fruits 7-9 mm treated with GA3 (VvS5)

text: pDONR


characteristic: INSDC center name

text: live

text: Generic

text: Thompson-seedless

text: Fruit

text: Vitis vinifera

text: BsrG I

text: BsrG I

text: Fruits 7-9 mm (VvS6)

text: pDONR

characteristic: vector


text: live

text: Generic

text: Thompson-seedless

characteristic: lab-host

text: Berry

text: Vitis vinifera

text: BsrG I

text: BsrG I

text: Berries 14mm with GA3 (VvS7)

text: pDONR


text: live

text: Generic

text: Thompson-seedless

text: Veraison

characteristic: dev-stage

text: Fruit

text: Vitis vinifera

text: BsrG I

text: BsrG I

text: Fruits Veraison (VvS8)

text: pDONR


text: live

text: Generic

text: Thompson-seedless

text: Berry

text: Vitis vinifera

text: BsrG I

text: BsrG I

text: Ripening Berries (VvS9)

text: pDONR


text: live

text: Generic

text: Thompson-seedless

text: Berry

text: Vitis vinifera

text: BsrG I

text: BsrG I

text: Ripening Berries with GA3 (VvS10)

text: pDONR


text: live

text: Generic

text: Carmenere

text: Cluster

text: Vitis vinifera

text: BsrG I

text: BsrG I

text: Clusters 4 cm (VvC3)

text: pDONR


text: live

text: Generic

text: Carmenere

text: Veraison

text: Cluster

text: Vitis vinifera

text: BsrG I

text: BsrG I

text: Berries veraison stage (VvC4)

text: pDONR


text: live

text: Generic

text: Carmenere

text: Berry

text: Vitis vinifera

text: BsrG I

text: BsrG I

text: Rippening Berries (last harvest) (VvC5)

text: pDONR


text: live

text: Generic

text: Thompson-seedless

text: Bud

text: Vitis vinifera

text: BsrG I

text: BsrG I

text: Buds (VvS2)

text: pDONR


text: live

text: Generic

text: Thompson-seedless

text: Fruit

text: Vitis vinifera

text: BsrG I

text: BsrG I

text: Fruits 2-3 mm with GA3 treatment (VvS3)

text: pDONR


text: live

text: Generic

text: Thompson-seedless

text: Fruit

text: Vitis vinifera

text: BsrG I

text: BsrG I

text: Fruits 2-3 mm (VvS4)

text: pDONR