text: Gyeongsang National Univ., Plant Molecular Biology and Biotechnology Research Center

text: live

text: Generic

text: Subtracted cDNA library from salt(NaCl)-treated whole plants

text: 7 day-old

text: Arabidopsis thaliana

text: Columbia

text: NaCl-treated Arabidopsis subtraction library


status: PUBLIC

characteristic: External Id

text: National Institute for Medical Research

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: peritoneal macrophages

tag: attribute

characteristic: cell type

text: cDNAs originated by differential display RT-PCR of Mycobacterium tuberculosis-infected macrophages

characteristic: description

text: blood

characteristic: organ

text: Mus musculus

characteristic: organism

text: BALB/c

characteristic: strain

text: macrophage

characteristic: tissue-type

text: Mouse Mycobacterium tuberculosis-infected macrophages

characteristic: title


text: Smith College Department of Biological Sciences

text: live

text: Generic

text: Filarial nematode parasite of humans. Third-stage larvae, L3, were isolated from infected black flies in Cameroon (forest strain). The L3 were cultured in 20% FCS in IMDM+ NCTC 135 and collected after day 1, 2, or 3 in culture. L3 of O. volvulus molt to fourth-stage larvae by day 5 in culture. mRNA was isolated from approximately 6000 molting larvae (mL3),2000 larvae from day 1, 2 or 3 in culture, and converted to double-stranded cDNA using reverse transcriptase and oligo(dT) followed by RNase H and DNA pol I. The library was constructed in the lambda Uni-Zap XR vector and has 1 x 10E6 independent recombinants and the average insert size is ~1200 bp. The library was constructed by Sara Lustigman and Michelle Lizotte-Waniewski in the Laboratory of Dr. S. A. Williams. The library is available from Dr. Sara Lustigman (email: slustigm@nybc.org).

text: molting L3

characteristic: dev-stage

text: XL1-Blue MRF'

characteristic: lab-host

text: Onchocerca volvulus

text: Eco RI

text: Xho I

text: Kumba, Cameroons

text: Onchocerca volvulus molting L3 larva cDNA (SL96MLW-OvmL3)

text: Phage

text: Lambda Uni-ZAP XR

characteristic: vector


characteristic: External Id

text: Cleveland Clinic Foundation

text: live

text: Generic

text: Homo sapiens

text: Human YAC-derived (I.H.Still)


text: The Institute for Genomic Research

characteristic: INSDC center name

text: live

text: Generic

text: adult

text: heart

text: Homo sapiens

text: Heart III

text: pcDNAI


text: The Institute for Genomic Research

characteristic: INSDC first public

text: live

text: Generic

text: adult

text: lung

text: Homo sapiens

text: EcoRI

text: XhoI

text: Lung

text: pBluescript SK-


text: The Institute for Genomic Research

characteristic: INSDC last update

text: live

text: Generic

text: adult

text: bone

text: Homo sapiens

text: EcoRI

text: XhoI

text: Bone VII

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

characteristic: INSDC status

text: Generic

text: adult

text: colon

text: Homo sapiens

text: EcoRI

text: XhoI

text: Colon I

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

characteristic: NCBI submission model

text: adult

text: liver

text: Homo sapiens

text: EcoRI

text: XhoI

text: Liver I

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

characteristic: NCBI submission package

text: Brain

text: Homo sapiens

text: neural blastoma

text: Brain IX

text: pcDNAI


text: The Institute for Genomic Research

text: live

text: Generic

text: adult

text: cornea

text: Homo sapiens

text: EcoRI

text: XhoI

text: Cornea I

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult

text: thymus

text: Homo sapiens

text: EcoRI

text: XhoI

text: Thymus I

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult

text: cornea

text: Homo sapiens

text: EcoRI

text: XhoI

text: Cornea II

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: trachea

text: Homo sapiens

text: EcoRI

text: XhoI

text: Trachea tumor

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: fetus

text: lung

text: Homo sapiens

text: EcoRI

text: XhoI

text: Fetal lung I

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult

text: skin

text: Homo sapiens

text: EcoRI

text: XhoI

text: Fibrosarcoma

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult

text: lung

text: Homo sapiens

text: EcoRI

text: XhoI

text: Lung tumor I

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: platelet

characteristic: cell type

text: adult

text: Homo sapiens

text: EcoRI

text: XhoI

text: Platelet

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult

text: skin

text: Homo sapiens

text: EcoRI

text: XhoI

text: Skin tumor I

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult

text: thymus

text: Homo sapiens

text: EcoRI

text: XhoI

text: Thymus III

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult

text: brain

text: Homo sapiens

text: EcoRI

text: XhoI

text: Frontal lobe

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult

text: lung

text: Homo sapiens

text: EcoRI

text: XhoI

text: Lung tumor II

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult

text: retina

text: Homo sapiens

text: EcoRI

text: HindIII

text: Retina I

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult

text: skin

text: Homo sapiens

text: EcoRI

text: XhoI

text: Skin tumor II

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: T-lymphocyte

text: Homo sapiens

text: EcoRI

text: XhoI

text: Supt cells

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: fetus

text: heart

text: Homo sapiens

text: EcoRI

text: XhoI

text: Fetal heart I

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: fetus

text: lung

text: Homo sapiens

text: EcoRI

text: XhoI

text: Fetal lung III

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: fetus

text: spleen

text: Homo sapiens

text: EcoRI

text: XhoI

text: Fetal spleen

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult

text: testis

text: Homo sapiens

text: EcoRI

text: XhoI

text: male

characteristic: sex

text: Testis I

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult

text: uterus

characteristic: organ

text: Homo sapiens

text: EcoRI

text: XhoI

text: female

text: Uterus

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: fetus

text: brain

text: Homo sapiens

characteristic: organism

text: EcoRI

text: XhoI

text: Fetal brain VI

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: fetus

text: heart

text: Homo sapiens

text: EcoRI

text: XhoI

text: Fetal heart II

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: fetus

text: liver

text: Homo sapiens

text: EcoRI

text: XhoI

text: Fetal liver IV

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

characteristic: cell-line

text: breast

text: Homo sapiens

text: EcoRI

text: XhoI

text: MCF7 cell line

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult

text: ovary

text: Homo sapiens

text: EcoRI

text: XhoI

text: female

text: Ovary II

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: fetus

text: brain

text: Homo sapiens

text: EcoRI

text: XhoI

text: Fetal brain III

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: Homo sapiens

text: EcoRI

text: XhoI

text: dura mater

characteristic: tissue-type

text: Fetal duramater

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: fetus

text: kidney

text: Homo sapiens

text: XhoI

text: EcoRI

text: Fetal kidney I

characteristic: title

text: pBluescript KS-


text: The Institute for Genomic Research

text: live

text: Generic

text: fetus

characteristic: dev-stage

text: liver

text: Homo sapiens

text: EcoRI

text: XhoI

text: Fetal liver III

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: infant

text: brain

text: Homo sapiens

text: HindIII

text: NotI

text: female

text: Infant brain

text: lafmid BA


text: The Institute for Genomic Research

text: live

text: Generic

text: prostate

text: Homo sapiens

text: EcoRI

text: XhoI

text: male

text: LNCAP cells I

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult

text: retina

text: Homo sapiens

text: EcoRI

text: XhoI

text: mixed

text: Retina II

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult

text: thymus

text: Homo sapiens

text: EcoRI

text: XhoI

text: Thymus tumor I

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult

text: thyroid gland

text: Homo sapiens

text: EcoRI

text: XhoI

text: Thyroid

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult

text: uterus

text: Homo sapiens

text: EcoRI

text: XhoI

text: Uterus tumor I

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: Homo sapiens

text: EcoRI

text: XhoI

text: CAMA1Ee cell line I

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: fetus

text: kidney

text: Homo sapiens

text: XhoI

text: EcoRI

text: Fetal kidney II

text: pBluescript KS-


text: The Institute for Genomic Research

text: live

text: Generic

text: Homo sapiens

text: EcoRI

text: XhoI

text: leiomyloid

text: Leiomyloid tumor

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult

text: Homo sapiens

text: EcoRI

text: XhoI

text: muscle

text: Skeletal muscle

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult

text: thymus

text: Homo sapiens

text: EcoRI

text: XhoI

text: Thymus tumor II

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult

text: uterus

text: Homo sapiens

text: EcoRI

text: XhoI

text: Uterus tumor II

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: Homo sapiens

text: EcoRI

text: XhoI

text: CAMA1Ee cell line II

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: embryo, 7 wks

text: Homo sapiens

text: EcoRI

text: XhoI

text: Embryo, 7 week

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: embryo, 9 wks

text: Homo sapiens

text: EcoRI

text: XhoI

text: Embryo, 9 week

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: fetus, 24 wks

text: bone

text: Homo sapiens

text: EcoRI

text: XhoI

text: Fetal bone

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult

text: lymph node

text: Homo sapiens

text: EcoRI

text: XhoI

text: Lymph node I

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: macrophage

text: adult

text: Homo sapiens

text: EcoRI

text: XhoI

text: Macrophage I

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: T-lymphocyte

text: Homo sapiens

text: EcoRI

text: XhoI

text: T-cell lymphoma

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult

text: testis

text: Homo sapiens

text: EcoRI

text: XhoI

text: male

text: Testis tumor

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult

text: thymus

text: Homo sapiens

text: EcoRI

text: XhoI

text: Thymus tumor III

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult

text: skin

text: Homo sapiens

text: EcoRI

text: XhoI

text: Epithilioid sarcoma

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: T-lymphocyte

text: Homo sapiens

text: EcoRI

text: XhoI

text: Jurkat T-cells I

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: T-lymphocyte

text: Homo sapiens

text: EcoRI

text: XhoI

text: Jurkat T-cells V

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult

text: liver

text: Homo sapiens

text: EcoRI

text: XhoI

text: male

text: liver

text: Liver III

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult

text: lymph node

text: Homo sapiens

text: EcoRI

text: XhoI

text: Lymph node II

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: macrophage

text: adult

text: Homo sapiens

text: EcoRI

text: XhoI

text: Macrophage II

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult

text: epididymis

text: Homo sapiens

text: EcoRI

text: XhoI

text: male

text: Epididymus

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult

text: esophagus

text: Homo sapiens

text: EcoRI

text: XhoI

text: Esophagus tumor

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult, 25 yrs

text: heart

text: Homo sapiens

text: EcoRI

text: XhoI

text: male

text: Heart I

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: T-lymphocyte

text: Homo sapiens

text: EcoRI

text: XhoI

text: Jurkat T-cells II

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: T-lymphocyte

text: Homo sapiens

text: EcoRI

text: XhoI

text: Jurkat T-cells VI

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: B-lymphocyte

text: Homo sapiens

text: EcoRI

text: XhoI

text: Namalwa B cells I

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult

text: pancreas

text: Homo sapiens

text: EcoRI

text: XhoI

text: Pancreas tumor I

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: embryo, 6 wks

text: Homo sapiens

text: EcoRI

text: XhoI

text: Embryo, 6 week II

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult

text: omentum

text: Homo sapiens

text: EcoRI

text: XhoI

text: Greater omentum IV

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: T-lymphocyte

text: Homo sapiens

text: EcoRI

text: XhoI

text: Jurkat T-cells III

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult, 40 yrs

text: liver

text: Homo sapiens

text: EcoRI

text: XhoI

text: male

text: Liver II

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: B-lymphocyte

text: Homo sapiens

text: EcoRI

text: XhoI

text: Namalwa B cells II

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult

text: ovary

text: Homo sapiens

text: EcoRI

text: XhoI

text: female

text: Ovarian cancer

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult

text: pancreas

text: Homo sapiens

text: EcoRI

text: XhoI

text: Pancreas tumor II

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult

text: pineal body

text: Homo sapiens

text: EcoRI

text: XhoI

text: Pineal gland I

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: embryo, 12 wks

text: Homo sapiens

text: EcoRI

text: XhoI

text: Embryo, 12 week I

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: fetus, 24 wks

text: Homo sapiens

text: EcoRI

text: XhoI

text: muscle

text: Fetal muscle

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult, 71 yrs

text: ovary

text: Homo sapiens

text: EcoRI

text: XhoI

text: female

text: Ovary I

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult

text: pancreas

text: Homo sapiens

text: EcoRI

text: XhoI

text: Pancreas tumor III

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult

text: pineal body

text: Homo sapiens

text: EcoRI

text: XhoI

text: Pineal gland II

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult, 23 yrs

text: spleen

text: Homo sapiens

text: EcoRI

text: XhoI

text: male

text: Spleen I

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult

text: Homo sapiens

text: EcoRI

text: XhoI

text: mixed

text: bone marrow

text: Bone marrow

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult

text: brain

text: Homo sapiens

text: EcoRI

text: XhoI

text: cerebellum

text: Cerebellum I

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: embryo, 12 wks

text: Homo sapiens

text: EcoRI

text: XhoI

text: Embryo, 12 week II

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: fetus

text: placenta

text: Homo sapiens

text: EcoRI

text: XhoI

text: placenta

text: Placenta II

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: fetus

text: placenta

text: Homo sapiens

text: EcoRI

text: EcoRI

text: placenta

text: Placenta I

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult, 19 yrs

text: thymus

text: Homo sapiens

text: EcoRI

text: XhoI

text: male

text: Thymus II

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult

text: brain

text: Homo sapiens

text: EcoRI

text: XhoI

text: cerebellum

text: Cerebellum II

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult

text: colon

text: Homo sapiens

text: EcoRI

text: XhoI

text: Colon adenocarcinoma IV

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult

text: Homo sapiens

text: EcoRI

text: XhoI

text: omentum

text: Greater omentum I

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult

text: omentum

text: Homo sapiens

text: EcoRI

text: XhoI

text: Greater omentum tumor

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult, 47 yrs

text: kidney

text: Homo sapiens

text: EcoRI

text: XhoI

text: female

text: Kidney I

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult

text: salivary gland

text: Homo sapiens

text: EcoRI

text: XhoI

text: Salivary gland

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: fetus, 19 wks

text: lung

text: Homo sapiens

text: EcoRI

text: XhoI

text: male

text: Fetal lung II

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult

text: Homo sapiens

text: EcoRI

text: XhoI

text: omentum

text: Greater omentum II

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult, 55 yrs

text: kidney

text: Homo sapiens

text: EcoRI

text: XhoI

text: female

text: Kidney IX

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: embryo, 6 wks

text: embryo

text: Homo sapiens

text: EcoRI

text: XhoI

text: Embryo, 6 week I

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult

text: Homo sapiens

text: EcoRI

text: XhoI

text: omentum

text: Greater omentum III

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult, 45 yrs

text: pancreas

text: Homo sapiens

text: EcoRI

text: XhoI

text: male

text: Pancreas I

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult

text: pituitary gland

text: Homo sapiens

text: EcoRI

text: XhoI

text: Pituitary gland

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: T-lymphocyte

text: adult

text: Homo sapiens

text: EcoRI

text: XhoI

text: Activated T-cells I

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: T-lymphocyte

text: adult

text: Homo sapiens

text: EcoRI

text: XhoI

text: Activated T-cells V

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: T-lymphocyte

text: adult

text: Homo sapiens

text: EcoRI

text: XhoI

text: Activated T-cells X

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: fetus, 17-18 wks

text: brain

text: Homo sapiens

text: EcoRI

text: EcoRI

text: female

text: Brain IV

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult, 45 yrs

text: pancreas

text: Homo sapiens

text: EcoRI

text: XhoI

text: male

text: Pancreas II

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: T-lymphocyte

text: adult

text: Homo sapiens

text: EcoRI

text: XhoI

text: Activated T-cells II

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: T-lymphocyte

text: adult

text: Homo sapiens

text: EcoRI

text: XhoI

text: Activated T-cells IV

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: T-lymphocyte

text: adult

text: Homo sapiens

text: EcoRI

text: XhoI

text: Activated T-cells IX

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: T-lymphocyte

text: adult

text: Homo sapiens

text: EcoRI

text: XhoI

text: Activated T-cells VI

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: T-lymphocyte

text: adult

text: Homo sapiens

text: EcoRI

text: XhoI

text: Activated T-cells XI

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: T-lymphocyte

text: adult

text: Homo sapiens

text: EcoRI

text: XhoI

text: Activated T-cells XX

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult

text: adrenal gland

text: Homo sapiens

text: EcoRI

text: XhoI

text: Adrenal gland tumor

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult

text: adrenal gland

text: Homo sapiens

text: EcoRI

text: XhoI

text: female

text: Adrenal gland

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult, 59 yrs

text: esophagus

text: Homo sapiens

text: EcoRI

text: XhoI

text: female

text: Esophagus

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: fetus, 24 wks

text: brain

text: Homo sapiens

text: EcoRI

text: XhoI

text: female

text: Fetal brain I

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: fetus, 24 wks

text: liver

text: Homo sapiens

text: EcoRI

text: XhoI

text: female

text: Fetal liver I

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult

text: lymph node

text: Homo sapiens

text: EcoRI

text: XhoI

text: Lymph node, subtracted

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult

text: parotid gland

text: Homo sapiens

text: EcoRI

text: XhoI

text: Paratid gland tumor

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult

text: small intestine

text: Homo sapiens

text: EcoRI

text: XhoI

text: Small intestine I

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: T-lymphocyte

text: adult

text: Homo sapiens

text: EcoRI

text: XhoI

text: Activated T-cells III

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: T-lymphocyte

text: adult

text: Homo sapiens

text: EcoRI

text: XhoI

text: Activated T-cells VII

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: T-lymphocyte

text: adult

text: Homo sapiens

text: EcoRI

text: XhoI

text: Activated T-cells XII

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: fetus, 24 wks

text: liver

text: Homo sapiens

text: EcoRI

text: XhoI

text: female

text: Fetal liver II

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: monocyte

text: adult

text: Homo sapiens

text: EcoRI

text: EcoRI

text: Monocytes, stimulated II

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult

text: small intestine

text: Homo sapiens

text: EcoRI

text: XhoI

text: Small intestine II

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: T-lymphocyte

text: adult

text: Homo sapiens

text: EcoRI

text: XhoI

text: Activated T-cells VIII

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult

text: endometrium

text: Homo sapiens

text: EcoRI

text: XhoI

text: female

text: Endometrial tumor

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: fetus, 24 wks

text: brain

text: Homo sapiens

text: EcoRI

text: EcoRI

text: female

text: Fetal brain II

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: blood cell, red

text: adult

text: Homo sapiens

text: EcoRI

text: XhoI

text: blood

text: Red blood cell

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult

text: heart

text: Homo sapiens

text: EcoRI

text: XhoI

text: Heart, subtracted (total cDNA)

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: fetus, 24 wks

text: kidney

text: Homo sapiens

text: XhoI

text: EcoRI

text: female

text: Fetal kidney III

text: pBluescript KS-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult, 71 yrs

text: ovary

text: Homo sapiens

text: EcoRI

text: XhoI

text: female

text: Ovary, subtracted

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult, 21 yrs

text: prostate

text: Homo sapiens

text: EcoRI

text: XhoI

text: male

text: Prostate gland I

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult, 64 yrs

text: Homo sapiens

text: EcoRI

text: XhoI

text: female

text: muscle

text: Rhabdomyosarcoma

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult, 23 yrs

text: spleen

text: Homo sapiens

text: EcoRI

text: XhoI

text: male

text: Spleen, subtracted

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult

text: brain

text: Homo sapiens

text: EcoRI

text: XhoI

text: corpus callosum

text: Corpus callosum I

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult

text: brain

text: Homo sapiens

text: EcoRI

text: EcoRI

text: frontal cortex

text: Frontal cortex II

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult, 17 yrs

text: prostate

text: Homo sapiens

text: EcoRI

text: EcoRI

text: male

text: Prostate gland V

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult

text: brain

text: Homo sapiens

text: EcoRI

text: XhoI

text: right hemisphere

text: Right hemisphere

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult

text: brain

text: Homo sapiens

text: EcoRI

text: XhoI

text: corpus callosum

text: Corpus callosum II

text: pBluescript SK-


text: Department of Genetics, The Hospital for Sick Children

text: live

text: Generic

text: embryo, 8 wks

text: Embryo, 8 weeks

text: Homo sapiens

text: EcoRI

text: XhoI

text: Embryo, 8 week I

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: fetus, 17-18 wks

text: brain

text: Homo sapiens

text: EcoRI

text: EcoRI

text: female

text: Fetal brain VII

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: epithelial cell

text: fetus

text: skin

text: Homo sapiens

text: EcoRI

text: XhoI

text: epithelium

text: Fetal skin

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult, 25 yrs

text: gall bladder

text: Homo sapiens

text: EcoRI

text: XhoI

text: female

text: Gall bladder I

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: fetus

text: heart

text: Homo sapiens

text: EcoRI

text: XhoI

text: Fetal heart, subtracted (total cDNA)

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult, 25 yrs

text: gall bladder

text: Homo sapiens

text: EcoRI

text: XhoI

text: female

text: Gall bladder II

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult, 74 yrs

text: brain

text: Homo sapiens

text: EcoRI

text: XhoI

text: male

text: hippocampus

text: Hippocampus I

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult

text: Homo sapiens

text: EcoRI

text: XhoI

text: adipose tissue, brown

text: Adipose tissue, brown

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: Homo sapiens

text: EcoRI

text: XhoI

text: lymphoid

text: CCRF-CEM cells, cyclohexamide treated I

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: fetus, 24 wks

text: brain

text: Homo sapiens

text: EcoRI

text: XhoI

text: female

text: Fetal brain, subtracted

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: fibroblast

text: HSC172 (16PDL)

text: fetal

text: lung

text: Homo sapiens

text: EcoRI

text: XhoI

text: HSC172 cells I

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult, 74 yrs

text: brain

text: Homo sapiens

text: EcoRI

text: XhoI

text: male

text: hippocampus

text: Hippocampus II

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult

text: liver

text: Homo sapiens

text: EcoRI

text: XhoI

text: Liver, subtracted (abundant clones) I

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult

text: parathyroid gland

text: Homo sapiens

text: EcoRI

text: XhoI

text: Parathyroid gland tumor I

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: B-lymphocyte

text: Homo sapiens

text: EcoRI

text: XhoI

text: Raji cells, cyclohexamide treated I

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: fibroblast

text: HSC172 (60PDL)

text: fetal

text: lung

text: Homo sapiens

text: EcoRI

text: XhoI

text: HSC172 cells II

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult

text: liver

text: Homo sapiens

text: EcoRI

text: XhoI

text: Liver, subtracted (abundant clones) II

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult

text: parathyroid gland

text: Homo sapiens

text: EcoRI

text: XhoI

text: Parathyroid gland tumor II

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult

text: thymus

text: Homo sapiens

text: EcoRI

text: XhoI

text: Thymus tumor, subtracted (total cDNA)

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult

text: Homo sapiens

text: EcoRI

text: XhoI

text: adipose tissue, white

text: Adipose tissue, white I

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: endothelial cell

text: adult

text: aorta

text: Homo sapiens

text: EcoRI

text: XhoI

text: Aorta endothelial cells

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult

text: Homo sapiens

text: EcoRI

text: XhoI

text: adipose tissue, white

text: Adipose tissue, white II

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult

text: liver

text: Homo sapiens

text: EcoRI

text: XhoI

text: male

text: liver

text: Liver, hepatocellular carcinoma

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: macrophage

text: adult

text: Homo sapiens

text: EcoRI

text: XhoI

text: Macrophage, subtracted (total cDNA)

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult, 70 yrs

text: Homo sapiens

text: EcoRI

text: XhoI

text: male

text: synovial membrane

text: Synovial membrane

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult, 74 yrs

text: brain

text: Homo sapiens

text: EcoRI

text: XhoI

text: male

text: left hemisphere

text: Left hemisphere

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult, 20 yrs

text: Homo sapiens

text: EcoRI

text: XhoI

text: female

text: synovial membrane

text: Synovial sarcoma

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: infant, 16 wks

text: adrenal gland

text: Homo sapiens

text: EcoRI

text: XhoI

text: male

text: Infant adrenal gland I

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: embryo, 6 wks

text: Homo sapiens

text: EcoRI

text: XhoI

text: Embryo, 6 week, subtracted (total cDNA) I

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: embryo, 7 wks

text: Homo sapiens

text: EcoRI

text: XhoI

text: Embryo, 7 week, subtracted (total cDNA) I

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: infant, 16 wks

text: adrenal gland

text: Homo sapiens

text: EcoRI

text: XhoI

text: male

text: Infant adrenal gland II

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: fetus

text: placenta

text: Homo sapiens

text: EcoRI

text: XhoI

text: placenta

text: Placenta, subtracted (total cDNA)

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: embryo, 6 wks

text: Homo sapiens

text: EcoRI

text: XhoI

text: Embryo, 6 week, subtracted (total cDNA) II

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: embryo, 7 wks

text: Homo sapiens

text: EcoRI

text: XhoI

text: Embryo, 7 week, subtracted (total cDNA) II

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult

text: pancreas

text: Homo sapiens

text: EcoRI

text: XhoI

text: Pancreas tumor, subtracted (abundant clones)

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: endothelial cell

text: adult

text: aorta

text: Homo sapiens

text: EcoRI

text: XhoI

text: Aorta endothelial cells, TNF alpha-treated

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult

text: brain

text: Homo sapiens

text: EcoRI

text: XhoI

text: right hemisphere

text: Right hemisphere, subtracted (mitochondria)

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: endothelial cell

text: fetus

text: umbilical vein

text: Homo sapiens

text: EcoRI

text: XhoI

text: Umbilical vein endothelial cells I

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: endothelial cell

text: fetus

text: umbilical vein

text: Homo sapiens

text: EcoRI

text: XhoI

text: Umbilical vein endothelial cells II

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: adult

text: pituitary gland

text: Homo sapiens

text: EcoRI

text: XhoI

text: Pituitary gland, subtracted (prolactin/growth hormone) II

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: infant, 16 wks

text: adrenal gland

text: Homo sapiens

text: EcoRI

text: XhoI

text: male

text: Infant adrenal gland, subtracted (total cDNA) I

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: Human colon adenocarcinoma;ATCC HTB 37

text: colon

text: Homo sapiens

text: EcoRI

text: XhoI

text: male

text: colon

text: Colon carcinoma (Caco-2) cell line I

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: KM12C(HCC)metastasis into mouse (liver)

text: Homo sapiens

text: EcoRI

text: XhoI

text: colon

text: HCC cell line (matastasis to liver in mouse)

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: Human colon adenocarcinoma;ATCC HTB 37

text: colon

text: Homo sapiens

text: EcoRI

text: XhoI

text: male

text: colon

text: Colon carcinoma (Caco-2) cell line II

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: epithelial cell

text: retina pigmented layer

text: Homo sapiens

text: EcoRI

text: HindIII

text: epithelium

text: Retinal pigment epithelium 0041 cell line

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: KM12C(HCC)-parental human colon carcinoma;Dukes B2

text: colon

text: Homo sapiens

text: EcoRI

text: XhoI

text: colon

text: Colon carcinoma (HCC) cell line

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: KM12C(HCC)metastasis into mouse (liver)

text: Homo sapiens

text: EcoRI

text: XhoI

text: colon

text: HCC cell line (matastasis to liver in mouse) II

text: pBluescript SK-


text: The Institute for Genomic Research

text: live

text: Generic

text: colon

text: Homo sapiens

text: EcoRI

text: XhoI

text: colon

text: Colon carcinoma (HCC) cell line II

text: pBluescript SK-


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: 1st strand cDNA was prepared from mRNA obtained from pooled 8-9 week (total) fetus material with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCTTAATTTTTTTTTTTTTTTTT 3']. Double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library went through one round of normalization, and was constructed by Bento Soares and M. Fatima Bonaldo.

characteristic: description

text: 8-9 weeks

characteristic: lab-host

text: Homo sapiens

text: Not I

text: Eco RI

text: phagemid (ampicillin resistant)


characteristic: External Id

text: live

text: Generic

text: Cloned unidirectionally from mRNA prepared from primitive streak embryonic tissue. Primer: Oligo dT. cDNAs were cloned into the XbaI/XhoI sites of pBluescript SK+ (Stratagene) using commercial linkers (NEB). Average insert size: 1.0 kb.

text: embryo (post-implantation)

text: embryo

text: Mus musculus

text: XbaI

text: XhoI

text: C57BL/6J x DBA/2J F1

characteristic: strain

text: primitive streak

text: Knowles Solter mouse 7 5dpc primitive streak

text: phagemid (ampicillin resistant)

text: pBluescript SK+

characteristic: vector


text: Washington University School of MedicineP

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Total RNAs were extracted from 11.5 dpc embryos (excluding placenta and yolk sac). The double-stranded cDNA was synthesized with an oligo (dT)-1 primer GAGAGAGACTAGTTCTAGATCGCGAGCGGCCGCTTTTTTTTTTTTTTTTTT 3'. The cDNAs were ligated to LL-Sal3A: 5' GCTATTGACGTCGACTATCC 3' and LL-Sal3B: 5' GGATAGTCGACGTCAAT 3'. The cDNAs were size-selected and amplified by long-range PCR using Ex Taq polymerase for 18 cycles. The PCR-amplifiable cDNA mixture went through one round of equalization and was digested with SalI/NotI and cloned into the SalI/NotI sites of the pSPORT1 plasmid vector (Life Technologies). The library was constructed by Dr. Minoru S. H. Ko and Dr. Xiaohong Wang.

text: embryo

text: Mus musculus

text: SalI

text: NotI

text: pooled

text: embryo

text: Ko mouse embryo 11 5dpc

text: phagemid (ampicillin resistant)


text: People's Hospital Beijing Medical University

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Total RNA was isolated from Breast Cancer cell lines(BCAP37-neo and BCAP37-GADD153). Then Differential Display RT-PCR was conducted. The cDNA PCR product of differentially expressional signifance was cloned and sequenced by employing PCR=AEII (3.9Kb) as vector

text: E.coli DH5a

text: Homo sapiens

text: Human Breast Cancer

text: Phagemid

text: PCR=AEII (3.9Kb)


text: The Chinese University of Hong Kong

text: live

text: Generic

text: The library is a gift from Prof. C.C. Liew, University of Toronto, Ontario, Canada. mRNA was purified from human fetal hearts (8-10 wks). cDNA was synthesized using a XhoI-Oligo dT adaptor primer. EcoRI adaptors were ligated, followed by digestion with XhoI, for directional cloning into predigested lambda ZAP Express.

text: E. coli

text: Homo sapiens

text: EcoRI

text: XhoI

text: Fetal heart, Lambda ZAP Express

text: Phage

text: Lambda ZAP


text: The Chinese University of Hong Kong

text: live

text: Generic

text: The library was bought from Clontech.

text: E. coli (Y1090)

text: Homo sapiens

text: EcoRI

text: XhoI

text: Adult heart, Lambda gt11

text: Phage

text: Lambda gt11


text: Charite University Hospital, Humboldt University of Berlin

text: live

text: Generic

text: monocytes

characteristic: cell type

text: Monocytes were prepared from peripheral blood by ficoll-hypaque, percoll and T-cell rosetting purification steps (purity: 96 %). mRNA was prepared from activated monocytes from a patient with rheumatoid arthritis. MRNA was reverse transcribed into cDNA using MuLV-RT. Using Eco-RI linkers cDNA was cloned into l-gt-11 vector arms. The cDNA library was screened by differential hybridization using radioactively marked ss-cDNA from activated and from non-activated monocytes.

text: blood

characteristic: organ

text: Homo sapiens

text: Eco-RI

text: male

characteristic: sex

text: RA-MO-I

text: Phage


text: live

text: Generic

text: Cloned unidirectionally from mRNA prepared from 800 blastocysts. Primer: SalI(dT): 5'-CGGTCGACCGTCGACCGTTTTTTTTTTTTTTT-3'. cDNAs were cloned into the NotI/SalI sites of a pSPORT vector (Life Technologies). Two different size selections: B1 (larger inserts) and B3.

text: embryo (pre-implantation)

text: embryo

text: Mus musculus

characteristic: organism

text: NotI

text: SalI

text: C57BL/6J x DBA/2J F1

text: blastocyst

characteristic: tissue-type

text: Knowles Solter mouse blastocyst B1

text: phagemid (ampicillin resistant)

text: pSPORT


text: San Francisco General Hospital-University of California, San Francisco

text: live

text: Generic

text: The C. parvum cDNA library was prepared by Drs. Norman J. Pieniazek, Michael J. Arrowood, Susan B. Slemenda, and Jan R. Mead at the Centers for Disease Control and Prevention (Atlanta, Georgia). Poly A+ RNA was separated from total C. parvum RNA using the Poly(A) Quik mRNA Isolation Kit from Stratagene. Directional cDNA was synthesized by first-strand priming with a Xho I-oligo d(T) linker-primer, second-stranding with RNase H and DNA polymerase I, ligation of EcoR I linkers, and digestion with Xho I, all using the Stratagene ZAP-cDNA synthesis kit. The cDNA was cloned into the EcoR I and Xho I sites of Lambda Uni-ZAP XR vector; the unamplified library was >95% recombinant and contained 3.8 X 10(6) independent clones. PCR analysis of 20 random clones indicated that the average insert size was ca. 1.1 kb.

text: sporozoite

characteristic: dev-stage

text: E. coli XL1 Blue MRF' Kan

text: Cryptosporidium parvum

text: EcoR I

text: Xho I

text: IOWA

characteristic: title

text: UniZAP XR


text: University of Padua

text: live

text: Generic

text: The library was constructed by G. Lanfranchi. This library is not subtracted nor normalized. The first strand cDNA was primed with a biotinylated oligo-dT-NotI primer (5'-biotin-AACCCGGCTCGAGCGGCCGCTTTTTTTTTTTTTTTTTT-3'). The ds cDNA was sonicated and size-selected in the range 350-550 bp. The 3' specific fragments were selected by streptavidin coated magnetic beads, ligated to non-palindromic BstXI adapters, NotI digested and directionally cloned into BstXI-NotI cut pcDNAII vector.

characteristic: description

text: Homo sapiens

text: BstXI

text: NotI

text: female

text: pectoral muscle (after mastectomy)

text: Plasmid

text: pcDNAII (Invitrogen)


characteristic: External Id

text: University of Sao Paulo - USP

text: live

text: Generic

text: epimastigote

text: cDNA library constructed with oligo dt primed epimastigote mRNA and cloned in pSPORT1 vector (Gibco BRL)

text: Trypanosoma cruzi

text: Cl-Brenner

text: T.cruzi epimastigote non-normalized cDNA Library


text: University of Sao Paulo - USP

text: live

text: Generic

text: epimastigote

text: cDNA library constructed with oligo dt primed epimastigote mRNA and cloned in pt7t318D phagemid with modified polylinker (Pharmacia)

text: Trypanosoma cruzi

text: Cl-Brenner

characteristic: strain

text: T.cruzi epimastigote normalized cDNA Library


text: live

text: Generic

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCGAAAGTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. RNA provided by Dr. Minoru Ko, Wayne State Univ. Library constructed and normalized by Bento Soares and M.Fatima Bonaldo.

text: 4 weeks

characteristic: lab-host

text: Mus musculus

text: Not I

text: Eco RI

text: male

text: heart

text: Soares mouse NbMH

text: phagemid (ampicillin resistant)

characteristic: vector


text: Wayne State University

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: Total RNAs were extracted from ectoplacental cone of 7.5-dpc embryos. The double-stranded cDNA was synthesized from total RNAs with an Oligo(dT) primer. The library was constructed by Minoru S. H. Ko.

text: embryonic day 7.5 postconception

text: embryo

text: Mus musculus

text: SalI

text: NotI

text: ectoplacental cone

text: Mouse 7.5 dpc embryo ectoplacental cone cDNA library

text: plasmid (ampicillin resistant)

text: pSPORT1 (Life Technologies)


text: Centers for Disease Control and Prevention

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: See: Salazar, C.E., et al. Insect Molecular Biology (1994), 3:1-13.

text: Anopheles gambiae

text: SalI

text: NotI

text: Male and Female

text: Anopheles gambiae adult pSport cDNA


text: Institute of Basic Medical sciences, Peking Union Medical College & Chinese Academy of Medical Sciences

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: E.coli K802

text: Homo sapiens

text: Human fetal brain QBoqin3

text: Phage

text: lambda phage DR2


text: Michigan State University

text: live

text: Generic

text: PRL2 cDNA library, MSU-DOE Plant Research Laboratory, Michigan State University; Newman,T. et al., Plant Physiology 106: 1241-1255 (1994)

text: Arabidopsis thaliana

text: Lambda-PRL2 (P.Kapranov)


text: Tufts University School of Veterinary Medicine

text: live

text: Generic

text: RT-PCR was performed using total RNA from pooled samples of whole animal or pooled hemolymph. cDNAs were ligated into pCR-Script Amp SK(+) vector and transformed in Epicurian coli XL-MRF' (Stratagene)

text: Penaeus vannamei

text: Penaeus vannamei cDNA library 1


text: live

text: Generic

text: RT-PCR was performed using total RNA isolated from hemolymph of individual Taura Syndrome Virus-challenged and survived shrimp. The RT-PCR amplified cDNA was ligated into pCR-Script Amp SK(+) vector and transformed in Epicurian coli XL-MRF' (Stratagene)

text: Penaeus vannamei

text: Penaeus vannamei cDNA library 2


text: Washington University School of MedicineP

text: live

text: Generic

text: Cloned unidirectionally from mRNA prepared from 13,500 2-cell stage embryos. Primer: SalI(dT): 5'-CGGTCGACCGTCGACCGTTTTTTTTTTTTTTT-3'. cDNAs were cloned into the MluI/SalI sites of a modified pBluescribe vector using commercial linkers (NEB). Average insert size: 1.2 kb.

text: embryo

characteristic: organ

text: Mus musculus

text: MluI

text: SalI

text: C57BL/6J x DBA/2J F1

text: embryo

text: Knowles Solter mouse 2 cell

text: phagemid (ampicillin resistant)

text: pBluescribe (modified)


text: Texas A&M University

text: live

text: Generic

text: Day 5 post-inoculation mRNAs prepared from Magnaporthe grisea grown at 23C in the dark with constant gyratory shaking 100 rpm in Vogel's minimal medium containing 0.5% isolated rice cell walls as the sole carbon source. Library provided by Sheng-Cheng Wu. Sequences were processed by one of two methods. Where a full-length alignment to the M. grisea genome sequence was available, the EST sequence was trimed according to the alignment, otherwise sequence quality was assessed using phredPhrap version 991019 and trimmed according to phd files (0.05) and for vector seqs.

text: Day 5 post-inoculation

text: Pyricularia grisea

text: EcoRI

text: XhoI

text: Mat1-1 hermaphrodite

characteristic: sex

text: Mycelium

text: RCW Lambda Zap Express Library

text: Phagemid

text: pBluescript excised from Lambda Zap Express


text: Yale University

text: live

text: Generic

text: Burns et al., Genes Dev. 8, 1087-1105 (1994). A yeast genomic DNA library was prepared in the vector pHSS6-Sal, and subjected to transposon mutagenesis with mTn-LEU2/lacZ. This mini-transposon carries lacZ sequences that lack an initiation codon; expression of lacZ is only provided by in-frame fusion to yeast coding sequence. The yeast genomic DNA carrying the mTn-LEU2/lacZ insertion was released from pHSS6-Sal by digestion with NotI, and transplaced back onto the yeast chromosome in diploid strain Y800. Yeast colonies expressing lacZ were screened using a color assay for beta-galactosidase activity. Using either yIP5 (Burns et al.) or pRSQ (Ross-Macdonald et al., Methods in Molecular and Cellular Biology 5, 298-308. 1995), a plasmid containing the fusion junction between genomic DNA and the lacZ end of mTn-LEU2/lacZ was recovered from each individual yeast transformant that expressed lacZ activity. These recovered plasmids comprise 'The blue guys library'. The fusion junction was then sequenced using a primer in the mTn's lacZ region. This sequence data was analyzed to identify the expressed ORF upstream of the fusion.

text: E.coli

text: Saccharomyces cerevisiae

characteristic: organism

text: Yeast::mTn-lacZ/LEU2 library

text: hybrid


text: Faculdade de Medicina de Ribeirao Preto - USP

text: live

text: Generic

text: mRNA was purified from adult couples of S. mansoni and the library was constructed and processed according to the manufacturer's instructions ( UNI- ZAP XR Vector, STRATAGENE)

text: adult

text: E. coli XL Blue1

text: Schistosoma mansoni

text: EcoRI

text: XhoI

text: NMRI

text: S. mansoni cDNA adult worm (R.Guerra-Sa)

text: pBluescript II SK+


text: Washington University School of Medicine

text: live

text: Generic

text: Cloned unidirectionally from mRNA prepared from 5000 unfertilized eggs. Primer: SalI(dT): 5'-CGGTCGACCGTCGACCGTTTTTTTTTTTTTTT-3'. cDNAs were cloned into the MluI/SalI sites of a modified pBluescribe vector using commercial linkers (NEB). Average insert size: 1.0 kb.

text: unfertilized egg

text: Mus musculus

text: MluI

text: SalI

text: C57BL/6J x DBA/2J F1

text: unfertilized egg

characteristic: tissue-type

text: Knowles Solter mouse unfertilized egg

text: phagemid (ampicillin resistant)

text: pBluescribe (modified)


text: live

text: Generic

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCGAATGGTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. RNA provided by Dr. Minoru Ko, Wayne State Univ. Library constructed and normalized by Bento Soares and M.Fatima Bonaldo.

text: 4 weeks

characteristic: dev-stage

text: mammary gland

text: Mus musculus

text: Not I

text: Eco RI

text: male

text: mammary gland

text: phagemid (ampicillin resistant)


text: Moredun Research Institute

text: live

text: Generic

text: The cDNA library was constructed by Diane Redmond (Moredun Research Institute, 408 Gilmerton Road, Edinburgh, EH17 7JH, UK). mRNA was prepared from adult Trichostrongylus vitrinus isolated from the small intestine of a lamb, 21 days after the lamb was infected with T. vitrinus infective L3. The mRNA was converted to first-strand cDNA using reverse transcriptase and an oligo(dT) primer. Following treatment with RNase H, the second cDNA strand was generated by using DNA polymerase I. The library had a total titre of 1.25 x 10E6 phage.

text: adult

text: Trichostrongylus vitrinus

text: male & female

text: Moredun

text: whole worm

text: Trichostrongylus vitrinus adult cDNA lambda gt11 library

characteristic: title


text: Nagoya University

text: live

text: Generic

text: Samsun NN

characteristic: cultivar

text: Nicotiana tabacum

text: Shoot apex

text: Tobacco shoot (MTamaoki)


characteristic: External Id

text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: Cloned unidirectionally from mRNA prepared from 2,000 8-cell stage embryos. Primer: SalI(dT): 5'-CGGTCGACCGTCGACCGTTTTTTTTTTTTTTT-3'. cDNAs were cloned into the NotI/SalI sites of a pSPORT vector (Life Technologies). Average insert size: 0.7 kb.

characteristic: description

text: 8 cell

text: embryo

text: Mus musculus

text: NotI

text: SalI

text: C57BL/6J x DBA/2J F1

text: embryo

text: Knowles Solter mouse 8 cell

text: phagemid (ampicillin resistant)

text: pSPORT


text: Cancer Institute, Chinese Academy of Medical Sciences

text: live

text: Generic

text: MA 891

characteristic: cell-line

text: Mus musculus

text: mammary tumor, metastasis

text: Mus musculus (Y.H.Zhang)


text: University of Munich

text: live

text: Generic

text: embryonic 2 - 14 hours

text: Drosophila melanogaster

text: Canton-S

characteristic: strain

text: Drosophila melanogaster Uni-ZAP XR library (Stratagene cat. #937602)


characteristic: INSDC center name

text: live

text: Generic

text: Cloned unidirectionally from mRNA prepared from 800 blastocysts. Primer: SalI(dT): 5'-CGGTCGACCGTCGACCGTTTTTTTTTTTTTTT-3'. cDNAs were cloned into the NotI/SalI sites of a pSPORT vector (Life Technologies). Two different size selections: B1 (larger inserts) and B3.

text: embryo (pre-implantation)

characteristic: lab-host

text: embryo

text: Mus musculus

text: NotI

text: SalI

text: C57BL/6J x DBA/2J F1

text: blastocyst

text: Knowles Solter mouse blastocyst B3

text: phagemid (ampicillin resistant)

text: pSPORT

characteristic: vector


text: Fred Hutchinson Cancer Research Center

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: PolyA+ RNA was isolated from the prostate of a healthy 25 year old male organ donor. 1st strand cDNA was primed with oligo(dT)Not1. Double-stranded cDNA was ligated to Sal1 adapters and directionally cloned into pSport1 (Life Technologies). Average insert size is 1.1kb.

text: prostate

text: Homo sapiens

text: male

text: PN001-Normal Human Prostate

text: plasmid


text: Dept. of Horticulture, University of Wisconsin

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: For details see Theoretical and Applied Genetics 90:607-614 (1995).

text: Seedling leaf

characteristic: organ

text: Allium cepa

text: Whole leaf

text: Onion seedling leaf cDNA library

text: Phage

text: lambda GT10


text: live

text: Generic

text: Bulb onion library prepared by Native Plants Inc, Salt Lake City, Utah.

text: DH5 alpha

text: Allium cepa

characteristic: organism

text: NPI Onion cDNA library

text: plasmid

text: pUC vector, probably pUC-13 derivative


text: Institute of Basic Medical sciences, Peking Union Medical College & Chinese Academy of Medical Sciences

text: live

text: Generic

text: E.Coli DH5a

text: Homo sapiens

text: Human fetal brain QBoqin4

text: Phagemid

text: pBS-SK


text: University of Berne

text: live

text: Generic

text: total RNA isolated from liquid cultures grown to late log phase in Middlebrook 7H9/ADC in an atmosphere of 5% oxygen, 5% CO2 and 90% Nitrogen. Random fragments were amplified from total RNA by rPCR with the primer UPN6 (5'-GCCGGAGCTCTGCAGAATTCNNNNNN-3') and cloned into EcoR1 site of pET24a (+). Clones from M.tb ribosomal RNA were detected by hybridization and removed.

text: late log phase

characteristic: dev-stage

text: Escherichia coli strain Novablue (Novagen)

text: Mycobacterium tuberculosis

text: PI, ATCC 51910

text: Mtb FOX (5% oxygen) expression library

text: plasmid

text: pET24a (+) (Novagen)


text: live

text: Generic

text: mRNA made from alveolar rhabdomyosarcoma, cDNA made by oligo-dT priming. Non-directionally cloned. Size-selected on agarose gel, average insert size 600 bp. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383.

text: Homo sapiens

text: alveolar rhabdomyosarcoma

characteristic: tissue-type

characteristic: title

text: plasmid (ampicillin resistant)


characteristic: External Id

text: live

text: Generic

text: 1st strand cDNA was prepared from 12 pooled bulk tumor samples and primed with a Not I - oligo(dT) primer. Double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library went through one round of normalization.

text: Homo sapiens

text: Not I

text: Eco RI

text: pooled

characteristic: sex

text: colon

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: mRNA made from Ewing's sarcoma, cDNA made by oligo-dT priming. Non-directionally cloned. Size-selected on agarose gel, average insert size 600 bp. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383.

characteristic: description

text: Homo sapiens

text: Ewing's sarcoma

text: plasmid (ampicillin resistant)


text: live

text: Generic

text: mRNA made from normal liver hepatocytes, cDNA made by oligo-dT priming. Non-directionally cloned. Size-selected on agarose gel, average insert size 600 bp. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383.

text: Homo sapiens

text: liver

text: plasmid (ampicillin resistant)


text: live

text: Generic

text: mRNA made from invasive hepatocellular carcinoma, cDNA made by oligo-dT priming. Non-directionally cloned. Size-selected on agarose gel, average insert size 600 bp. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383.

text: Homo sapiens

text: male

text: liver

text: plasmid (ampicillin resistant)


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: mRNA made from liposarcoma, cDNA made by oligo-dT priming. Non- directionally cloned. Size-selected on agarose gel, average insert size 600 bp. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383.

text: Homo sapiens

text: liposarcoma

text: plasmid (ampicillin resistant)


text: live

text: Generic

text: mRNA made from invasive ovarian tumor, cDNA made by oligo-dT priming. Non-directionally cloned. Size-selected on agarose gel, average insert size 600 bp. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383.

text: Homo sapiens

text: female

text: ovary

text: plasmid (ampicillin resistant)


text: live

text: Generic

text: mRNA made from prostatic intraepithelial neoplasia (low-grade), cDNA made by oligo-dT priming. Non-directionally cloned. Size-selected on agarose gel, average insert size 600 bp. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383.

text: Homo sapiens

text: male

text: prostate

text: plasmid (ampicillin resistant)


text: live

text: Generic

text: mRNA made from invasive thyroid tumor, cDNA made by oligo-dT priming. Non-directionally cloned. Size-selected on agarose gel, average insert size 600 bp. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383.

text: Homo sapiens

text: thyroid

text: plasmid (ampicillin resistant)


text: live

text: Generic

text: mRNA made from invasive kidney tumor, cDNA made by oligo-dT priming. Non-directionally cloned. Size-selected on agarose gel, average insert size 600 bp. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383.

text: Homo sapiens

text: kidney

text: plasmid (ampicillin resistant)


text: live

text: Generic

text: mRNA made from normal prostatic epithelial cells, cDNA made by oligo-dT priming. Non-directionally cloned. Size-selected on agarose gel, average insert size 600 bp.

text: Homo sapiens

text: male

text: prostate

text: plasmid (ampicillin resistant)


text: live

text: Generic

text: mRNA made from invasive prostate tumor, cDNA made by oligo-dT priming. Non-directionally cloned. Size-selected on agarose gel, average insert size 600 bp.

text: Homo sapiens

text: male

text: prostate

text: plasmid (ampicillin resistant)


text: ERATO, JST

text: live

text: Generic

text: Xenopus laevis

text: ovary

text: Xenopus laevis ovary (S.Hirohashi)


text: live

text: Generic

text: 1st strand cDNA was prepared from pooled bulk breast tumor tissue, and was then primed with a Not I - oligo(dT) primer. Double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library is not normalized. (The normalized version of this library is NCI_CGAP_Br2.) Library was constructed by Bento Soares and M. Fatima Bonaldo.

text: Homo sapiens

text: female, pooled

text: breast

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: 1st strand cDNA was prepared from pooled bulk breast tumor tissue, and was then primed with a Not I - oligo(dT) primer. Double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. This library is the normalized version of NCI_CGAP_Br1.1. Library was constructed by Bento Soares and M. Fatima Bonaldo.

text: Homo sapiens

text: female, pooled

text: breast

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: 1st strand cDNA was prepared from pooled colon tumor tissue, and was then primed with a Not I - oligo(dT) primer. Double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. This library is not normalized. Library constructed by Bento Soares and M. Fatima Bonaldo.

text: Homo sapiens

text: pooled

text: colon

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' AACTGGAAGAATTCGCGGCCGCAATATTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library went through one round of normalization. Library constructed by Bento Soares and M. Fatima Bonaldo.

text: 15 wk post natal

text: whole brain

text: Homo sapiens

text: Not I

text: Eco RI

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: RNA was prepared from a pool of 6 anonymous Wilms' tumor RNAs. RNA was prepared by acid-phenol, followed by one round of oligo dT selection. cDNA library preparation was with the BRL/Life Tech. Superscript Plasmid system. An oligo-dT NotI primer for first strand synthesis generated gcggccgccc(t)n at the 3' end of the clones. A 5' SalI adaptor was used with sequence 5'-gtcgacccacgcgtccg-3'. Resulting cDNAs were size selected (average size 2 kb), NotI digested, and ligated into NotI/SalI-cut pSPORT1. Library was constructed by Dr. Manfred Gessler.

text: Homo sapiens

text: SalI

text: NotI

text: pooled (6)

text: Gessler Wilms tumor

text: plasmid (ampicillin resistant)


text: Washington University School of Medicine

text: live

text: Generic

text: 1st strand cDNA was primed with a Not I - oligo(dT)15 primer [5'pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT3'], on mRNA from pooled 26 somite zebrafish embryos; double-stranded cDNA was ligated to Sal I adaptors (BRL), digested with Not I and cloned into the Not I and Sal I sites of the pSPORT1 vector (BRL). Library was constructed by Matthew Clark (Lehrach lab; ICRF, London and Max Planck Institut fuer Molekulare Genetik, Berlin) and was not biochemically normalised. 70,000 clones from this library were arrayed on high density filters and subsequently screened by oligonucleotide hybridization fingerprinting to identify unique or minimally redundant clones for more intensive analysis.

text: XL1-blue MRF

text: Danio rerio

text: NotI

text: SalI

text: mixed

text: pooled 26-somite embryos

text: Zebrafish ICRFzfls

text: plasmid (ampicillin resistant)


text: NRC-Institute for Marine Biosciences

text: live

text: Generic

text: cDNA library construction was described by Zhou and Ragan (1993) Curr. Genet. 23: 483-489. Organism name synonym = Gracilaria verrucosa.

text: whole thalli

text: Gracilaria gracilis

text: Oslo, Norway

characteristic: strain

text: Zhou and Ragan 1993

text: lambda-ZAPII


text: University of Delaware

characteristic: INSDC center name

text: live

text: Generic

text: Con A-activated splenic T cell

characteristic: cell type

text: E.coli TOP10 F'

text: Gallus gallus

text: male

text: chicken activated T cell cDNA


text: live

text: Generic

text: Cloned unidirectionally from mRNA prepared from primitive streak embryonic tissue. Primer: Oligo dT. cDNAs were cloned into the XbaI/XhoI sites of pBluescript SK+ (Stratagene) using commercial linkers (NEB). Average insert size: 1.0 kb.

text: embryo (post-implantation)

characteristic: lab-host

text: Mus musculus

text: XbaI

text: XhoI

text: C57BL/6J x DBA/2J F1

text: Knowles Solter mouse 11 5day limb bud

text: phagemid (ampicillin resistant)

text: pBluescript SK+

characteristic: vector


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Cloned unidirectionally from mRNA prepared from primitive streak embryonic tissue. Primer: Oligo dT. cDNAs were cloned into the XbaI/XhoI sites of pBluescript SK+ (Stratagene) using commercial linkers (NEB). Average insert size: 0.5 kb.

text: embryo (post-implantation)

text: Mus musculus

text: XbaI

text: XhoI

text: C57BL/6J x DBA/2J F1

text: Knowles Solter mouse inner cell mass

text: phagemid (ampicillin resistant)

text: pBluescript SK+


text: Stanford University

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: cell-line

text: Differentially Expressed Genes Identified in an Estrogen Receptor-Positive Breast Carcinoma Cell Line Using Suppression Subtractive Hybridization

text: E. coli [XL1-Blue MRF']

text: breast

text: Homo sapiens

text: EcoRI

text: EcoRII

text: female

text: breast adenocarcinoma

text: Human MCF7 cDNA subtracted with MDA-MB-231 cDNA

text: Plasmid

text: pCR II [Invitrogen]


text: Jawaharlal Nehru University

text: live

text: Generic

characteristic: NCBI submission package

text: trophozoite

text: trophozoite

text: Entamoeba dispar

characteristic: organism

text: SAW 142

text: Entamoeba dispar cDNAs


text: Crop and Food Research

text: live

text: Generic

text: Southport White Globe

characteristic: cultivar

text: Re-sprouted bulb

characteristic: dev-stage

text: bulb and sprouting foliage leaf

characteristic: organ

text: Allium cepa

text: Sprouting onion bulb library

text: Plasmid

text: Lambda Zap


characteristic: External Id

text: live

text: Generic

text: Pukekohe Longkeeper

text: From re-sprouted bulb

text: Root

text: Allium cepa

text: Sprouting onion root library

characteristic: title

text: Plasmid

text: Lambda Zap


text: live

text: Generic

text: Cloned unidirectionally from mRNA prepared from 13,500 2-cell stage embryos. Primer: SalI(dT): 5'-CGGTCGACCGTCGACCGTTTTTTTTTTTTTTT-3'. cDNAs were cloned into the MluI/SalI sites of a modified pBluescribe vector using commercial linkers (NEB).

characteristic: description

text: embryo (post-implantation)

text: Mus musculus

text: MluI

text: SalI

text: C57BL/6J x DBA/2J F1

characteristic: strain

text: Knowles Solter mouse E6 5d whole embryo

text: phagemid (ampicillin resistant)

text: pBluescribe (modified)


text: Washington University School of MedicineP

characteristic: INSDC center name

text: live

text: Generic

text: Tissue obtained from 8 week old mouse. Colon was harvested 72 hours after irradiation with 1400 Gys. 1st strand cDNA was primed with a Not I - oligo(dT) primer [5'TGTTACGAATCTGAAGTGGGAGCGGCCGCCCTTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Eco RI adaptors [AATTCGGATCCTTG], digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library constructed by Bob Barstead.

text: 8 weeks

text: Mus musculus

text: EcoRI

text: NotI

text: FVB/N

text: Barstead mouse irradiated colon MPLRB7

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: 1st strand cDNA was prepared from normal prostate bulk tissue, and was then primed with a Not I - oligo(dT) primer. Double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library is normalized, and was constructed by Bento Soares and M. Fatima Bonaldo.

characteristic: lab-host

text: prostate

text: Homo sapiens

text: male

characteristic: sex

text: normal prostate

characteristic: tissue-type

text: phagemid (ampicillin resistant)


text: Faculty of Science, Ain Shams University

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Library provided by Dr. M. Saber TBRI,Cairo,Egypt.

text: cercarial

text: Schistosoma mansoni

text: Mixed

text: S. mansoni cercarial Lambda Zap


text: National Human Genome Research Institute

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: stromal fibroblast

characteristic: cell type

text: mixed

text: Homo sapiens

text: EcoRI

text: XhoI

text: Male and Female

text: bone marrow

text: Human Bone Marrow Stromal Fibroblast

text: pBluescript

characteristic: vector


text: Institute of Basic Medical sciences/Peking Union Medical College & Chinese Academy of Medical Sciences(PUMC & CAMS)

text: live

text: Generic

characteristic: NCBI submission package

text: fetal

text: E.coli DH5a

text: Homo sapiens

characteristic: organism

text: liver

text: Human fetal liver (S.Xue)

text: Phagemid

text: pBluescript SK


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Bulk lung tumor. 5' adaptor sequence: 5' GAATTCGGCACGAG 3' 3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3' Average insert size: 1.1 kb.

text: SOLR (kanamycin resistant)

text: lung

characteristic: organ

text: Homo sapiens

text: EcoRI

text: XhoI

text: lung tumor

text: phagemid (ampicillin resistant)

text: Bluescript SK-


characteristic: External Id

text: live

text: Generic

text: Cloned unidirectionally from mRNA prepared from 800 blastocysts. Primer: SalI(dT): 5'-CGGTCGACCGTCGACCGTTTTTTTTTTTTTTT-3'. cDNAs were cloned into the NotI/SalI sites of a pSPORT vector (Life Technologies).

text: embryo

characteristic: dev-stage

text: Mus musculus

text: NotI

text: SalI

text: C57BL/6J x DBA/2J F1

text: Knowles Solter mouse embryonic stem cell

text: phagemid (ampicillin resistant)

text: pSPORT


text: live

text: Generic

characteristic: cell-line

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACGAATCTGAAGTGGGAGCGGCCGCCCTTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Eco RI adaptors [AATTCGGATCCTTG], digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library constructed by Bob Barstead. The C2C12 cell line (available from ATCC, catalog # CRL-1772) differentiates rapidly, forming contractile myotubes and producing characteristic muscle proteins.

characteristic: description

text: Mus musculus

text: EcoRI

text: NotI

text: Barstead mouse myotubes MPLRB5

characteristic: title

text: phagemid (ampicillin resistant)


text: Washington University School of Medicine

characteristic: INSDC center name

text: live

text: Generic

text: Mature bradyzoites were obtained from infected mouse brains by percoll density centrifugation. The original library was constructed by Steve Parmley, Palo Alto Medical Foundation. cDNAs were synthesized by priming with oligo d(T) and directionally cloned into the EcoRI/NotI sites of lambda gt11. Warning: the library contains a small percentage of host cDNAs derived from mouse cells. Inserts from this cDNA library were excised with Not1 and EcoR1, size selected in a range of 0.7 - 2.0 kb and subcloned into Bluescript II SK- (Adrian Hehl, Ian Manger and John Boothroyd, Stanford University)

text: Bradyzoite

characteristic: lab-host

text: Toxoplasma gondii

text: EcoRI

text: NotI

characteristic: strain

text: TgME49 invivo Bradyzoite cDNA size selected

text: Plasmid

text: Bluescript II SK-


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

characteristic: INSDC first public

text: live

text: Generic

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACGAATCTGAAGTGGGAGCGGCCGCCCTTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Eco RI adaptors [AATTCGGATCCTTG], digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library constructed by Bob Barstead.

text: 7 day juvenile

text: Mus musculus

text: EcoRI

text: NotI

text: FVB/N

text: Barstead mouse proximal colon MPLRB6

text: phagemid (ampicillin resistant)


characteristic: INSDC last update

text: live

text: Generic

text: mRNA made from metastatic prostate lesion of the bone, cDNA made by oligo-dT priming. Non-directionally cloned. Size-selected on agarose gel, average insert size 600 bp. Library made by D. Krizman, NIH.

text: Homo sapiens

text: male

characteristic: sex

text: metastatic prostate bone lesion

characteristic: tissue-type

text: plasmid (ampicillin resistant)


text: University of Arizona

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Xanthi

characteristic: cultivar

text: ESTs generated by direct sequencing of nuclear transcripts using a new technique termed NEST (Nuclear Expressed Sequence Tag) Analysis.

text: leaf

text: Nicotiana tabacum

text: NEST tobacco leaf 01


text: University of Arizona

text: live

text: Generic

characteristic: NCBI submission package

text: Xanthi

text: root

text: Nicotiana tabacum

characteristic: organism

text: NEST tobacco root 01


characteristic: External Id

text: live

text: Generic

text: mRNA made from metastatic prostate lesion of the liver, cDNA made by oligo-dT priming. Non-directionally cloned. Size-selected on agarose gel, average insert size 600 bp. Library made by D. Krizman, NIH.

text: Homo sapiens

text: male

text: prostate metastasis, liver

text: plasmid (ampicillin resistant)

characteristic: vector


text: live

text: Generic

text: 1st strand cDNA was prepared from normal prostate bulk tissue, and was then primed with a Not I - oligo(dT) primer. Double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library is not normalized. Library was constructed by Bento Soares and M. Fatima Bonaldo.

text: prostate

characteristic: organ

text: Homo sapiens

text: male

text: normal prostate

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: mRNA made from normal prostatic epithelial cells, cDNA made by oligo-dT priming. Non-directionally cloned. Size-selected on agarose gel, average insert size 600 bp. Library made by D. Krizman, NIH.

characteristic: description

text: prostate

text: Homo sapiens

text: male

text: normal prostatic epithelial cells

text: plasmid (ampicillin resistant)


text: live

text: Generic

text: mRNA made from invasive prostate tumor, cDNA made by oligo-dT priming. Non-directionally cloned. Size-selected on agarose gel, average insert size 600 bp. Library made by D. Krizman, NIH.

text: prostate

text: Homo sapiens

text: male

text: invasive prostate tumor

characteristic: title

text: plasmid (ampicillin resistant)


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Two pooled bulk adrenal adenomas. 5' adaptor sequence: 5' GAATTCGGCACGAG 3' 3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3' Average insert size: 1.6 kb.

text: SOLR (kanamycin resistant)

text: adrenal gland

text: Homo sapiens

text: EcoRI

text: XhoI

text: adrenal adenoma

text: phagemid (ampicillin resistant)

text: Bluescript SK-


text: University of Tsukuba

characteristic: INSDC center name

text: live

text: Generic

text: slug

characteristic: dev-stage

text: Dictyostelium discoideum

text: Dictyostelium discoideum SL (H.Urushihara)


text: Sidney Kimmel Cancer Center

text: live

text: Generic

text: keratinocyte

characteristic: cell type

text: derived from RAP-PCR products

text: neonate

text: Homo sapiens

text: Human RAP-PCR products


characteristic: INSDC first public

text: live

text: Generic

text: mRNA made from human bone marrow stroma, cDNA made by oligo-dT priming. Directionally cloned. Size-selected for average insert size >0.5 kb. Library constructed by Dr. Marian Young and Dr. Pamela Gehron Robey (NIDCR). Library supplied by Dr. Libin Jia (NHGRI)

text: mixed

text: XL1-Blue MRF'/SOLR

characteristic: lab-host

text: Homo sapiens

text: EcoRI

text: XhoI

text: mixed

text: bone marrow stroma

text: Human bone marrow stromal cells

text: plasmid (ampicillin resistant)

text: pBluescript


characteristic: INSDC last update

text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Pooled pancreatic islet tumors. 5' adaptor sequence: 5' GAATTCGGCACGAG 3' 3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3' Average insert size: 1.2 kb.

text: SOLR (kanamycin resistant)

text: pancreas

text: Homo sapiens

text: EcoRI

text: XhoI

text: islet cell tumor

text: phagemid (ampicillin resistant)

text: Bluescript SK-


text: live

text: Generic

text: mRNA made from flow-sorted CD34+/CD38- hematopoietic stem cells, cDNA made by oligo-dT priming. Non-directionally cloned. Size-selected on agarose gel, average insert size 600 bp. cDNA Library Preparation: David B. Krizman, Ph.D. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383. cDNA Library Arrayed by: Greg Lennon, Ph.D. DNA Sequencing by: Washington University Genome Sequencing Center

text: bone marrow

text: Homo sapiens

text: stem cells

characteristic: tissue-type

text: plasmid (ampicillin resistant)


text: live

characteristic: INSDC status

text: Generic

text: mRNA made from prostate intraepithelial neoplasia (high-grade), cDNA made by oligo-dT priming. Non-directionally cloned. Size-selected on agarose gel, average insert size 600 bp.

text: adult

text: prostate

text: Homo sapiens

text: male

characteristic: sex

text: prostatic intraepithelial neoplasia - high grade

text: plasmid (ampicillin resistant)


text: live

text: Generic

characteristic: NCBI submission model

text: mRNA made from prostatic intraepithelial neoplasia (high-grade), cDNA made by oligo-dT priming. Non-directionally cloned. Size-selected on agarose gel, average insert size 600 bp. cDNA Library Preparation: David B. Krizman, Ph.D. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383. cDNA Library Arrayed by: Greg Lennon, Ph.D. DNA Sequencing by: Washington University Genome Sequencing Center

text: prostate

text: Homo sapiens

text: male

text: plasmid (ampicillin resistant)


text: live

text: Generic

characteristic: NCBI submission package

text: Cloned unidirectionally. Primer: Oligo dT. Bulk colon villous adenoma. 5' adaptor sequence: 5' GAATTCGGCACGAG 3' 3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3' Average insert size: 1.1 kb.

text: SOLR (kanamycin resistant)

text: colon

text: Homo sapiens

text: EcoRI

text: XhoI

text: tumor

text: phagemid (ampicillin resistant)

text: Bluescript SK-


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Multiple colon tumors. 5' adaptor sequence: 5' GAATTCGGCACGAG 3' 3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3' Average insert size: 1.1 kb.

text: SOLR (kanamycin resistant)

text: colon

text: Homo sapiens

characteristic: organism

text: EcoRI

text: XhoI

text: tumor

text: phagemid (ampicillin resistant)

text: Bluescript SK-


characteristic: External Id

text: School of Biochemistry & Genetics, University of Newcastle upon Tyne

text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Bulk germ cell tumor. 5' adaptor sequence: 5' GAATTCGGCACGAG 3' 3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3' Average insert size: 1.2 kb.

text: SOLR (kanamycin resistant)

text: Homo sapiens

text: EcoRI

text: XhoI

text: germ cell tumor

text: phagemid (ampicillin resistant)

text: Bluescript SK-


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: prostate

text: Homo sapiens

text: male

text: low-grade prostatic neoplasia

text: plasmid (ampicillin resistant)

characteristic: vector


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Two pooled bulk Schwannoma tumors. 5' adaptor sequence: 5' GAATTCGGCACGAG 3' 3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3' Average insert size: 1.2 kb.

text: SOLR (kanamycin resistant)

text: Homo sapiens

text: EcoRI

text: XhoI

text: Schwannoma tumor

text: phagemid (ampicillin resistant)

text: Bluescript SK-


text: National Institute of Animal Industry

text: live

text: Generic

text: young

text: liver

characteristic: organ

text: Sus scrofa

text: male

text: landrace

characteristic: strain

text: Porcine liver cDNA (A.Ozawa)


text: Sidney Kimmel Cancer Center

text: live

text: Generic

text: erythroleukemia

characteristic: cell-line

text: Homo sapiens

text: Human erythroleukemia RAP-PCR products

characteristic: title


text: Washington University School of Medicine

characteristic: INSDC center name

text: live

text: Generic

text: Library was constructed in Stratagene Uni-Zap XR lambda vector with Zap-cDNA synthesis kit: EcoRI linkers were added to 5' ends and XhoI linkers were added to 3' ends and resulting cDNAs were ligated into Uni-Zap XR lambda vector. In vivo mass excision was used to generate cDNAs in the pBluescript SK vector. Initial phage library amplified from 900,000 independent phage, 99% recombinants (by X-gal staining), 90% inserts (by PCR amplification), 1.7 kb average insert (range 0.8 - 3.4 kb).

characteristic: description

text: Danio rerio

text: EcoRI

text: XhoI

text: mixed

text: pooled 15-19 hour zebrafish embryos

text: Appel Eisen zebrafish embryo 15 19hr

text: plasmid (ampicillin resistant)

text: pBluescript SK(-)


text: University of Tsukuba

characteristic: INSDC first public

text: live

text: Generic

text: slug

characteristic: dev-stage

text: Dictyostelium discoideum

text: Dictyostelium discoideum SS (H.Urushihara)


text: Institut Pasteur

characteristic: INSDC last update

text: live

text: Generic

text: promyelocytic HL60 cell line

text: XL1 Blue

characteristic: lab-host

text: Homo sapiens

text: Human promyelocytic HL60 cell line (S.Herblot)

text: lambda ZAPII


text: UMD-New Jersey Medical School

text: live

characteristic: INSDC status

text: Generic

text: fibroblast

characteristic: cell type

text: HS74 and its SV40-transformed sublines

text: fetal

text: Homo sapiens

text: Human immortalized fibroblasts (H.L.Ozer)


text: U78 INSERM

text: live

text: Generic

characteristic: NCBI submission model

text: Library construction: Ripperger,J.A. et al. J. Biol. Chem. (1995) 270(50):29998-30006

text: adult

text: liver

text: Rattus norvegicus

text: Sprague-Dawley

text: liver

characteristic: tissue-type

text: Rat liver ESTs (E.Olivier)

text: phage

text: lambda ZAPII


text: Mount Sinai Hospital

text: live

text: Generic

characteristic: NCBI submission package

text: lung

text: Homo sapiens

characteristic: organism

text: lung carcinoma

text: Human lung carcinoma (E.P.Diamandis)


characteristic: External Id

text: Texas A&M University

text: live

text: Generic

text: spleen

text: Bos taurus

text: spleen

text: Bovine spleen 5'-stretch (Clontech cat. no. BL3000b)


text: live

text: Generic

text: A mini-library was made by cloning products derived from RNA-arbitrarily primed PCR (RAP PCR) profiles into the pUC 18 vector. Reverse transcription of three hour schistosomula mRNA was primed with ENR2 and subsequent PCR amplification was performed in the presence of the same primer

text: 3 hr schistosomula

text: Schistosoma mansoni

text: ENR2/ENR2 three hour schistosomula worm

text: plasmid

characteristic: vector


text: Graduate School of Science, Kyoto University

text: live

text: Generic

text: tailbud embryo

text: embryo

characteristic: organ

text: Halocynthia roretzi

text: tail

text: Halocynthia roretzi tailbud embryo


text: University of Edinburgh

text: live

text: Generic

text: Brugia malayi is a lymphatic filarial nematode parasite of humans. mRNA was prepared from third stage larvae of Brugia malayi isolated from the peritoneal cavity of jirds six days after infection. The mRNA was converted to double stranded cDNA using reverse transcriptase and oligo(dT) followed by RNase H and DNApol I. The library had 2 x 10E5 independent recombinants and average insert size was 900 base pairs. The library was constructed by Michelle Lizotte-Waniewski. The library is available from The Filarial Genome Project Resource Center: contact Dr. S.A. Williams, Clark Science Center, Smith College, Northampton, MA 01063 USA phone +1 413 585 3826 fax +1 413 585 3786 email genome@smith.edu.

text: third stage larvae, six days after infection

text: E. coli XL1-Blue

text: Brugia malayi

text: Eco R I (5' end)

text: Xho I (3' end)

text: mixed

characteristic: sex

text: TRS Labs

characteristic: strain

text: Brugia malayi day 6 post-infection third stage larvae SAW96MLW-BmL3d6

text: Phage

text: lambdaZapII (UniZap XR)


text: The Institute for Genomic Research

characteristic: INSDC center name

text: live

text: Generic

text: poly(A)+ RNA was purified from adult whole snails. cDNA was constructed using an oligo-dT primer and directionally cloned using the Lambda ZAP II Vector Kit by Stratagene.

text: whole body

text: Biomphalaria glabrata

text: EcoRI

text: XhoI

text: whole body

text: B. glabrata adult whole body

characteristic: title

text: pBluescript SK-


text: The Institute for Genomic Research

characteristic: INSDC first public

text: live

text: Generic

text: Total RNA was purified from adult cerebral ganglia exposed to S. mansoni miracidia. cDNA was constructed using an oligo-dT primer and directionally cloned using the Lambda ZAP II Vector Kit by Stratagene.

characteristic: description

text: brain

text: Biomphalaria glabrata

text: EcoRI

text: XhoI

text: cerebral ganglia

text: B. glabrata adult cerebral ganglia

text: pBluescript SK-


characteristic: INSDC last update

text: live

text: Generic

text: 1st strand cDNA was prepared from RER+ colon tumor, and was then primed with a Not I - oligo(dT) primer. Double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library is normalized. Library was constructed by Bento Soares and M. Fatima Bonaldo (N-Soares4).

text: colon

text: Homo sapiens

text: colon tumor RER+

text: phagemid (ampicillin resistant)


text: North Carolina State University

text: live

text: Generic

text: The library was made from immature xylem from the underside of inclined stems of differentiating wood. A mixture of four genotypes were used. Oligo-dT prime cDNA was directionally cloned into the EcoRI-XhoI lambda-ZAP vector arms

text: SOLR

characteristic: lab-host

text: Pinus taeda

text: EcoRI

text: XhoI

text: Coastal plain loblolly pine from North Carolina

text: Xylem

text: Loblolly pine C

text: lambda-ZAP


text: North Carolina State University

text: live

characteristic: INSDC status

text: Generic

text: The library was made from immature xylem from the side of inclined stems of differentiating wood. A mixture of four genotypes were used. Oligo-dT primed cDNA was directionally cloned into the EcoRI-XhoI lambda-ZAP vector arms

text: SOLR

text: Pinus taeda

text: EcoRI

text: XhoI

text: Xylem

text: Loblolly pine N

text: lambda-ZAP


text: North Carolina State University

text: live

text: Generic

text: The result of subtraction of C library with N library. Immature xylem from the underside of inclined stems of differentiating compression wood was subtracted with immature xylem from the side of inclined stems of differentiating wood. A mixture of four genotypes were used. Oligo-dT primed cDNA was directionally cloned into the EcoRI-XhoI lambda-ZAP vector arms

text: SOLR

text: Pinus taeda

text: EcoRI

text: XhoI

text: Xylem

text: Loblolly pine CA

text: lambda-ZAP


text: North Carolina State University

text: live

text: Generic

text: The result of subtraction of library N with library C. Immature xylem from the side of inclined stems of differentiating wood was subtracted with immature xylem from underside of inclined stems of differentiating compression wood. A mixture of four genotypes were used. oligo-dT primed cDNA was directionally cloned into the EcoRI-XhoI lambda-ZAP vector arms

text: SOLR

text: Pinus taeda

text: EcoRI

text: XhoI

text: Xylem

text: Loblolly pine NA

text: lambda-ZAP


text: Tokyo University of Marine Science and Technology, Graduate School of Marine Science and Technology

text: live

text: Generic

text: liver

text: Paralichthys olivaceus

text: liver

text: Japanese flounder liver


text: live

text: Generic

text: spleen

text: Paralichthys olivaceus

text: spleen

text: Japanese flounder spleen


text: The Hospital for Sick Children

text: live

text: Generic

text: Homo sapiens

text: Human chromosome 14


text: National University of Singapore

text: live

text: Generic

text: mRNA was purified from 4 adult zebrafish. cDNA was unidirectionaly cloned into the lambda ZAPII vector using the Stratagene's Lambda Uni-ZAP XR cDNA cloning system.

text: Danio rerio

text: EcoRI

text: XhoI

text: zebrafish adult

text: Phage

text: Lambda Uni-ZAP XR


text: National University of Singapore

text: live

text: Generic

text: mRNA was purified from mixed stages of zebrafish embryos from 6 hours postfertilization to hatching. cDNA was unidirectionally cloned into the lambda ZAPII vector using the Stratagene's Lambda Uni-ZAP XR cDNA cloning system.

text: mixed from 6 hours postfertilization to hatching

characteristic: dev-stage

text: embryo

text: Danio rerio

text: EcoRI

text: XhoI

text: embryonic

text: zebrafish embryonic (subtraction)

text: Phage

text: Lambda Uni-ZAP XR


text: Eleanor Roosevelt Institute

text: live

text: Generic

characteristic: NCBI submission model

text: fetal

text: brain

text: Homo sapiens

text: brain

text: Human fetal brain chromosome 21


text: Lawrence Berkeley National Lab

text: live

text: Generic

text: Constructed using Stratagene ZAP-cDNA Synthesis kit. Oligo dT-primed and directionally cloned at EcoRI and XhoI in BlueScript SK(+/-)

text: adult

text: XL1 Blue MRF

text: head--brain & sensory organ

text: Drosophila melanogaster

text: EcoRI

text: XhoI

text: male and female

text: HL Drosophila Head

text: BlueScript SK


text: Lawrence Berkeley National Lab

text: live

text: Generic

text: newly eclosed females: germarium-stage 6

text: XL1 Blue MRF

text: ovary

text: Drosophila melanogaster

text: EcoRI

text: XhoI

text: female

text: GM Drosophila Ovary

text: BlueScript SK


text: The Scripps Research Institute

text: live

text: Generic

text: Homo sapiens

text: Human chromosome 6


text: Institute of Radiation Medicine

text: live

text: Generic

text: neoplastic transformed epithelial

characteristic: cell type

text: Rattus norvegicus

text: Wistar

text: trachea

characteristic: tissue-type

text: Rat trachea neoplastic


text: The Natural History Museum

text: live

text: Generic

characteristic: NCBI submission package

text: The library was prepared commercially by Stratagene in Uni-ZAP XR using mRNA from in vitro cultured, 7 day schistosomula derived from mechanically-transformed cercaria (cultured by Richard Harrop, University of York). mRNA was reverse transcribed using oligo dT as primer and the cDNA was directionally cloned between the EcoRI and XhoI sites of the vector. Primary titre = 1.8x10e6, amplified titre = 7.7x10e9. The library contains 9.5% non recombinant clones and significant contamination with E. coli sequences (approx 20% of clones).

text: lung stage (7 day, in vitro cultured schistosolula)

text: Biomphalaria glabrata

text: whole organism

text: Schistosoma mansoni

characteristic: organism

text: mixed

text: Puerto Rica

text: York-Harrop-lung-A

text: Phage

text: Uni-ZAP XR


characteristic: External Id

text: The Natural History Museum

text: live

text: Generic

text: The library was constructed by Mohamed Saber (Theodor Bilharz Research Institute, Cairo) from cercariae of an Egyptian strain of Schistosoma mansoni, maintained in Mesocricetus auratus (Golden Hamster) and Biomphalaria alexandrina . Total mRNA was isolated using the Guanadine thiocyanate / phenol-chloroform method and poly (A)+ RNA purified by affinity chromatography on an oligo d(T) column . The cDNA library was unidirectionally constructed, by ligation of the restricted, double stranded cDNA into the EcoRI and XhoI restriction sites of Lambda ZapII.

text: cercaria

text: Mesocricetus auratus (definitive), Biomphalaria alexandrina (intermediate)

text: whole organism

text: Schistosoma mansoni

text: mixed

text: Egyptian

text: TBRI-Saber-cercaria-A

text: Phage

text: Lambda Zap II

characteristic: vector


text: live

text: Generic

text: Eggs of Schistosoma japonicum (Philippine strain) were purified from livers of rabbits about 50 days after infection with cercariae at the College of Public Health, University of The Philippines, Manila, The Philippines. Subsequently, at The Queensland Institute of Medical Research, Brisbane, Australia, messenger RNA was isolated from lysates of the S. japonicum eggs by oligo dT chromatography, first strand cDNA synthesis from the mRNA was primed with an oligo dT-XhoI primer and synthesized with M-MLV reverse transcriptase, and second strand synthesis was accomplished with RNAse H and T4 DNA polymerase. The double stranded cDNA was ligated into the phagemid vector UNI ZAP XR (Stratagene), and the ligation products packaged using reagents from Stratagene. The library was amplified using BB4 strain E. coli bacteria. The library was constructed by Sharon Day, Rolando Tan, Wilfred Tiu, and Paul Brindley who consider that it contains sequences expressed by immature and mature eggs of S. japonicum. The library is named 'PME-SD.96'

text: Egg

text: Rabbit

text: Schistosoma japonicum

text: EcoRI

text: Xho I

text: Mixed sex

characteristic: sex

text: Philippine strain

text: Whole organism

text: Phagemid

text: Lambda ZAP-II XR (Stratagene)


text: Kyushu University School of Medicine

text: live

text: Generic

text: Chromosome 7 specific library containing human DNA drived from GM10791 somatic cell hybrid

text: Homo sapiens

text: GM10791 library (Eric D. Green)