text: Helicos BioSciences Corporation

text: live

text: Generic

text: Un-fragmented Total RNA fraction isolated from human Brain RNA purchased from Clontech and converted into cDNA using oligo-dT primers and processed using Helicos Digital Gene Expression protocol

text: Homo sapiens

text: Sample from Homo sapiens


status: PUBLIC

characteristic: External Id

text: Helicos BioSciences Corporation

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

characteristic: INSDC secondary accession

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

characteristic: SRA accession

text: Un-fragmented Total RNA fraction isolated from human K562 RNA purchased from Ambion and converted into cDNA using oligo-dT primers and processed using Helicos Digital Gene Expression protocol

characteristic: description

text: Homo sapiens

characteristic: organism

text: Sample from Homo sapiens

characteristic: title


text: Helicos BioSciences Corporation

text: live

text: Generic

text: Un-fragmented RiboMinus RNA fraction isolated from human Liver RNA purchased from Clontech and converted into cDNA using Random primers and processed using Helicos RNAseq protocol, technical replica 1 , scientist 1

text: Homo sapiens

text: Sample from Homo sapiens


text: Helicos BioSciences Corporation

text: live

text: Generic

text: Un-fragmented polyA RNA fraction isolated from human K562 RNA purchased from Ambion using magnetic bead-based purification kit and converted into cDNA using Random primers and processed using Helicos RNAseq protocol, technical replica 1

text: Homo sapiens

text: Sample from Homo sapiens


text: Helicos BioSciences Corporation

text: live

text: Generic

text: Un-fragmented polyA RNA fraction isolated from human Liver RNA purchased from Clontech using magnetic bead-based purification kit and converted into cDNA using Random primers and processed using Helicos RNAseq protocol, technical replica 1 , scientist 2

text: Homo sapiens

text: Sample from Homo sapiens


text: Helicos BioSciences Corporation

text: live

text: Generic

text: Un-fragmented polyA RNA fraction isolated from human Liver RNA purchased from Clontech using magnetic bead-based purification kit and converted into cDNA using Random primers and processed using Helicos RNAseq protocol, technical replica 2 , scientist 2

text: Homo sapiens

text: Sample from Homo sapiens


text: Helicos BioSciences Corporation

text: live

text: Generic

text: Un-fragmented Total RNA fraction isolated from human K562 RNA purchased from Ambion and converted into cDNA using Random primers and processed using Helicos RNAseq protocol

text: Homo sapiens

text: Sample from Homo sapiens


text: Helicos BioSciences Corporation

text: live

text: Generic

text: Fragmented polyA RNA fraction isolated from human Liver RNA purchased from Clontech and converted into cDNA using Random primers and processed using Helicos RNAseq protocol, technical replica 3 , scientist 2

text: Homo sapiens

text: Sample from Homo sapiens


text: Helicos BioSciences Corporation

text: live

text: Generic

text: Un-fragmented RiboMinus RNA fraction isolated from human Liver RNA purchased from Clontech and converted into cDNA using Random primers and processed using Helicos RNAseq protocol, technical replica 2 , scientist 1

text: Homo sapiens

text: Sample from Homo sapiens


text: Helicos BioSciences Corporation

text: live

text: Generic

text: Un-fragmented Total RNA fraction isolated from human Liver RNA purchased from Clontech and converted into cDNA using Random primers and processed using Helicos RNAseq protocol

text: Homo sapiens

text: Sample from Homo sapiens


text: Helicos BioSciences Corporation

text: live

text: Generic

text: Un-fragmented RiboMinus RNA fraction isolated from human Brain RNA purchased from Clontech and converted into cDNA using Random primers and processed using Helicos RNAseq protocol, technical replica 1

text: Homo sapiens

text: Sample from Homo sapiens


text: Helicos BioSciences Corporation

text: live

text: Generic

text: Un-fragmented RiboMinus RNA fraction isolated from human K562 RNA purchased from Ambion and converted into cDNA using Random primers and processed using Helicos RNAseq protocol, technical replica 2

text: Homo sapiens

text: Sample from Homo sapiens


text: Helicos BioSciences Corporation

text: live

text: Generic

text: Un-fragmented Total RNA fraction isolated from human Brain RNA purchased from Clontech and converted into cDNA using Random primers and processed using Helicos RNAseq protocol

text: Homo sapiens

text: Sample from Homo sapiens


text: Helicos BioSciences Corporation

text: live

text: Generic

text: Un-fragmented polyA RNA fraction isolated from human Liver RNA purchased from Clontech using magnetic bead-based purification kit and converted into cDNA using Random primers and processed using Helicos RNAseq protocol, technical replica 3 , scientist 2

text: Homo sapiens

text: Sample from Homo sapiens


text: Helicos BioSciences Corporation

text: live

text: Generic

text: Fragmented polyA RNA fraction isolated from human Liver RNA purchased from Clontech and converted into cDNA using Random primers and processed using Helicos RNAseq protocol, technical replica 2 , scientist 2

text: Homo sapiens

text: Sample from Homo sapiens


text: Helicos BioSciences Corporation

text: live

text: Generic

text: Un-fragmented polyA RNA fraction isolated from human Liver RNA purchased from Clontech using magnetic bead-based purification kit and converted into cDNA using Random primers and processed using Helicos RNAseq protocol, technical replica 2 , scientist 1

text: Homo sapiens

text: Sample from Homo sapiens


text: Helicos BioSciences Corporation

text: live

text: Generic

text: Un-fragmented Total RNA fraction isolated from human Liver RNA purchased from Clontech and converted into cDNA using oligo-dT primers and processed using Helicos Digital Gene Expression protocol

text: Homo sapiens

text: Sample from Homo sapiens


text: Helicos BioSciences Corporation

text: live

text: Generic

text: Un-fragmented polyA RNA fraction isolated from human K562 RNA purchased from Ambion using magnetic bead-based purification kit and converted into cDNA using Random primers and processed using Helicos RNAseq protocol, technical replica 2

text: Homo sapiens

text: Sample from Homo sapiens


text: Helicos BioSciences Corporation

text: live

text: Generic

text: Un-fragmented polyA RNA fraction isolated from human Liver RNA purchased from Clontech using oligo-dT cellulose, one round and converted into cDNA using Random primers and processed using Helicos RNAseq protocol

text: Homo sapiens

text: Sample from Homo sapiens


text: Helicos BioSciences Corporation

text: live

text: Generic

text: Un-fragmented RiboMinus RNA fraction isolated from human K562 RNA purchased from Ambion and converted into cDNA using Random primers and processed using Helicos RNAseq protocol, technical replica 1

text: Homo sapiens

text: Sample from Homo sapiens


text: Helicos BioSciences Corporation

text: live

text: Generic

text: Un-fragmented RiboMinus RNA fraction isolated from human Liver RNA purchased from Clontech and converted into cDNA using Random primers and processed using Helicos RNAseq protocol, technical replica 1 , scientist 1

text: Homo sapiens

text: Sample from Homo sapiens


text: Helicos BioSciences Corporation

text: live

text: Generic

text: Un-fragmented Total RNA fraction isolated from human K562 RNA purchased from Ambion and converted into cDNA using oligo-dT primers and processed using Helicos Digital Gene Expression protocol

text: Homo sapiens

text: Sample from Homo sapiens


text: Helicos BioSciences Corporation

text: live

text: Generic

text: Un-fragmented Total RNA fraction isolated from RNA from untreated HL60 (ATCC; CCL-240) cells and converted into cDNA using Selected primers and processed using Helicos RNAseq protocol

text: Homo sapiens

text: Sample from Homo sapiens


text: Helicos BioSciences Corporation

text: live

text: Generic

text: Un-fragmented Total RNA fraction isolated from RNA from HL60 (ATCC; CCL-240) cells treated with retinoic acid (tRET) to induce differentiation (0.1%DMSO + 1 micromolar tRET for 5 days) and converted into cDNA using Random primers and processed using Helicos RNAseq protocol

text: Homo sapiens

text: Sample from Homo sapiens


text: Helicos BioSciences Corporation

text: live

text: Generic

text: Un-fragmented Total RNA fraction isolated from RNA from untreated HL60 (ATCC; CCL-240) cells and converted into cDNA using Random primers and processed using Helicos RNAseq protocol

text: Homo sapiens

text: Sample from Homo sapiens


text: Helicos BioSciences Corporation

text: live

text: Generic

text: Un-fragmented polyA RNA fraction isolated from RNA from untreated HL60 (ATCC; CCL-240) cells using magnetic bead-based purification kit and converted into cDNA using Random primers and processed using Helicos RNAseq protocol

text: Homo sapiens

text: Sample from Homo sapiens


text: Helicos BioSciences Corporation

text: live

text: Generic

text: Un-fragmented Total RNA fraction isolated from RNA from untreated HL60 (ATCC; CCL-240) cells and converted into cDNA using oligo-dT primers and processed using Helicos Digital Gene Expression protocol

text: Homo sapiens

text: Sample from Homo sapiens


text: Helicos BioSciences Corporation

text: live

text: Generic

text: Un-fragmented polyA RNA fraction isolated from RNA from HL60 (ATCC; CCL-240) cells treated with retinoic acid (tRET) to induce differentiation (0.1%DMSO + 1 micromolar tRET for 5 days) using magnetic bead-based purification kit and converted into cDNA using Random primers and processed using Helicos RNAseq protocol

text: Homo sapiens

text: Sample from Homo sapiens


text: Helicos BioSciences Corporation

text: live

text: Generic

text: Un-fragmented Total RNA fraction isolated from RNA from HL60 (ATCC; CCL-240) cells treated with retinoic acid (tRET) to induce differentiation (0.1%DMSO + 1 micromolar tRET for 5 days) and converted into cDNA using oligo-dT primers and processed using Helicos Digital Gene Expression protocol

text: Homo sapiens

text: Sample from Homo sapiens


name: 1129259764024 Yersinia pestis KIM D27 GYP14-ILLUMINA-PE Sample

text: J. Craig Venter Institute

text: live

text: Generic

text: Hybrid 454 and illumina sequencing of the Y. pestis KIM D27 genome

text: Yersinia pestis KIM D27

text: KIM D27

tag: attribute

characteristic: strain

text: Sample from Yersinia pestis KIM D27


Brain

text: Manufacturer

characteristic: Ambion

text: University of Queensland

text: live

text: Generic

characteristic: catalogue #

text: FLASHPage purified small RNA (~15-40nt) from Ambion FirstChoice Human Brain Total RNA (AM7962)

characteristic: fraction

text: RNA

characteristic: molecule

text: Homo sapiens

text: Sample from Homo sapiens


Heart

text: Manufacturer

characteristic: External Id

text: University of Queensland

text: live

text: Generic

text: FLASHPage purified small RNA (~15-40nt) from Ambion FirstChoice Human Heart Total RNA (AM7966)

text: RNA

text: Homo sapiens

text: Sample from Homo sapiens


Kidney

text: Manufacturer

text: University of Queensland

characteristic: INSDC center name

text: live

text: Generic

text: FLASHPage purified small RNA (~15-40nt) from Ambion FirstChoice Human Kidney Total RNA (AM7976)

text: RNA

text: Homo sapiens

text: Sample from Homo sapiens


Liver

text: Manufacturer

text: University of Queensland

characteristic: INSDC first public

text: live

text: Generic

text: FLASHPage purified small RNA (~15-40nt) from Ambion FirstChoice Human Liver Total RNA (AM7960)

text: RNA

text: Homo sapiens

text: Sample from Homo sapiens


Lung

text: Manufacturer

text: University of Queensland

characteristic: INSDC last update

text: live

text: Generic

text: FLASHPage purified small RNA (~15-40nt) from Ambion FirstChoice Human Lung Total RNA (AM7968)

text: RNA

text: Homo sapiens

text: Sample from Homo sapiens


Ovary

text: Manufacturer

text: University of Queensland

characteristic: INSDC secondary accession

text: live

text: Generic

text: FLASHPage purified small RNA (~15-40nt) from Ambion FirstChoice Human Ovary Total RNA (AM6974)

text: RNA

text: Homo sapiens

text: Sample from Homo sapiens


Placenta

text: Manufacturer

text: University of Queensland

text: live

characteristic: INSDC status

text: Generic

text: FLASHPage purified small RNA (~15-40nt) from Ambion FirstChoice Human Placenta Total RNA (AM7950)

text: RNA

text: Homo sapiens

text: Sample from Homo sapiens


Spleen

text: Manufacturer

text: University of Queensland

text: live

text: Generic

characteristic: NCBI submission model

text: FLASHPage purified small RNA (~15-40nt) from Ambion FirstChoice Human Spleen Total RNA (AM7970)

text: RNA

text: Homo sapiens

text: Sample from Homo sapiens


Testes

text: Manufacturer

text: University of Queensland

text: live

text: Generic

characteristic: NCBI submission package

text: FLASHPage purified small RNA (~15-40nt) from Ambion FirstChoice Human Testes Total RNA (AM7972)

text: RNA

text: Homo sapiens

text: Sample from Homo sapiens


Thymus

text: Manufacturer

text: University of Queensland

text: live

text: Generic

characteristic: SRA accession

text: FLASHPage purified small RNA (~15-40nt) from Ambion FirstChoice Human Thymus Total RNA (AM7964)

text: RNA

text: Homo sapiens

text: Sample from Homo sapiens


Abmorella-Sample

text: University of Georgia

text: live

text: Generic

text: WGS of the Amborella genome

characteristic: description

text: Amborella trichopoda

text: WGS of the Amborella genome


text: National Center for Genome Resources

text: live

text: Generic

text: Cicer arietinum

characteristic: organism

text: Sample from Cicer arietinum


text: dbGaP

text: live

text: Generic

text: DNA

characteristic: analyte type

characteristic: biospecimen repository

characteristic: biospecimen repository sample id

text: Blood

characteristic: body site

text: GRU-MDS

text: No

characteristic: is tumor

text: SNV Aggregate (.MAF)

characteristic: molecular data type

text: Homo sapiens

text: male

characteristic: sex

text: Tumor vs. Matched-Normal

characteristic: study design

characteristic: study name

characteristic: submitted sample id

characteristic: submitted subject id

characteristic: submitter handle

text: Non-tumor DNA sample from Blood of a human male participant in the dbGaP study Towards a Genomic Understanding of Myeloma

characteristic: title


characteristic: External Id

text: dbGaP

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

characteristic: INSDC secondary accession

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: DNA

text: Bone Marrow

text: GRU-MDS

text: Yes

text: SNV Aggregate (.MAF)

text: Homo sapiens

text: male

text: Tumor vs. Matched-Normal

text: Tumor DNA sample from Bone Marrow of a human male participant in the dbGaP study Towards a Genomic Understanding of Myeloma


text: dbGaP

text: live

text: Generic

characteristic: SRA accession

text: DNA

characteristic: analyte type

characteristic: biospecimen repository

characteristic: biospecimen repository sample id

text: Bone Marrow

characteristic: body site

text: DS-CCRD-MDS

text: Yes

characteristic: is tumor

text: SNV Aggregate (.MAF)

text: Homo sapiens

characteristic: organism

text: male

text: Tumor vs. Matched-Normal

text: Towards a Genomic Understanding of Myeloma


text: dbGaP

text: live

text: Generic

text: DNA

text: Bone Marrow

text: GRU-MDS

text: Yes

text: SNV Aggregate (.MAF)

characteristic: molecular data type

text: Homo sapiens

text: male

characteristic: sex

text: Tumor vs. Matched-Normal

characteristic: study design

characteristic: study name

characteristic: submitted sample id

characteristic: submitted subject id

characteristic: submitter handle


characteristic: External Id

text: dbGaP

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

characteristic: INSDC secondary accession

text: live

characteristic: INSDC status

text: Generic

text: DNA

text: Bone Marrow

text: GRU-MDS

text: Yes

text: SNV Aggregate (.MAF)

text: Homo sapiens

text: female

text: Tumor vs. Matched-Normal

text: Tumor DNA sample from Bone Marrow of a human female participant in the dbGaP study Towards a Genomic Understanding of Myeloma

characteristic: title


text: dbGaP

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

characteristic: SRA accession

text: DNA

characteristic: analyte type

characteristic: biospecimen repository

characteristic: biospecimen repository sample id

text: Blood

characteristic: body site

text: DS-CCRD-MDS

text: No

text: SNV Aggregate (.MAF)

text: Homo sapiens

text: male

text: Tumor vs. Matched-Normal


text: dbGaP

text: live

text: Generic

text: DNA

text: Bone Marrow

text: DS-CCRD-MDS

text: Yes

characteristic: is tumor

text: SNV Aggregate (.MAF)

characteristic: molecular data type

text: Homo sapiens

characteristic: organism

text: female

characteristic: sex

text: Tumor vs. Matched-Normal

characteristic: study design

characteristic: study name

characteristic: submitted sample id


characteristic: External Id

text: dbGaP

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

characteristic: INSDC secondary accession

text: live

text: Generic

text: DNA

text: Blood

text: GRU-MDS

text: No

text: SNV Aggregate (.MAF)

text: Homo sapiens

text: male

text: Tumor vs. Matched-Normal

characteristic: submitted subject id

characteristic: submitter handle


text: dbGaP

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

characteristic: SRA accession

text: DNA

characteristic: analyte type

characteristic: biospecimen repository

text: Blood

text: GRU-MDS

text: No

text: SNV Aggregate (.MAF)

text: Homo sapiens

text: female

text: Tumor vs. Matched-Normal

text: Non-tumor DNA sample from Blood of a human female participant in the dbGaP study Towards a Genomic Understanding of Myeloma

characteristic: title


text: dbGaP

text: live

text: Generic

text: DNA

characteristic: biospecimen repository sample id

text: Blood

characteristic: body site

text: DS-CCRD-MDS

text: No

characteristic: is tumor

text: SNV Aggregate (.MAF)

characteristic: molecular data type

text: Homo sapiens

characteristic: organism

text: female

characteristic: sex

text: Tumor vs. Matched-Normal

characteristic: study design


characteristic: External Id

text: School of Biology and Basic Medical Sciences, Soochow University

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Conchospores of thalli of Porphyra yezoensis Ueda

characteristic: organ

text: Neopyropia yezoensis

text: Conchospores of Porphyra yezoensis


text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Leafy vegetative cells of thalli of Porphyra yezoensis Ueda

text: Neopyropia yezoensis

text: Leafy vegetative cells of Porphyra yezoensis


text: Henan Agricultural University

text: live

text: Generic

text: Whole plant (root, stem and leaf)

characteristic: cell type

characteristic: cultivar

text: To study molecular mechanism underline continuously cropping obstacles of Rehmannia glutinosa, SSH was used to screen key genes related to the obstacles. The forward cDNA library was constructed by taking the cDNA of continuous cropping Rehmannia glutinosa (two-year replanting) whole plants as the tester and that of one-year planting as the driver.

characteristic: description

text: the tuberous root expansion stage

characteristic: dev-stage

text: Rehmannia glutinosa

text: Forward cDNA library of Rehmannia glutinosa L.

text: pMD18-T (TaKaRa)

characteristic: vector


text: Henan Agricultural University

text: live

text: Generic

text: Whole plant (root, stem and leaf)

text: To study molecular mechanism underline continuously cropping obstacles of Rehmannia glutinosa, SSH was used to screen key genes related to the obstacles. The reverse cDNA library was constructed by taking the cDNA of normal planting Rehmannia glutinosa (one-year replanting) whole plants as the tester and that of continuous cropping (two-year planting) as the driver.

text: the tuberous root expansion stage

text: Rehmannia glutinosa

text: Reverse cDNA library of Rehmannia glutinosa L.

characteristic: title

text: pMD18-T (TaKaRa)


text: University of California Merced

text: live

text: Generic

text: Sebastes goodei

characteristic: organism

text: testes, ovary

characteristic: tissue-type

text: Sebastes goodei mixed gonad library


text: Universidade Federal de Goias

text: live

text: Generic

text: Mycelium 12, 24, 36 and 48 hours after grown in liquid medium suplemented with Fusarium solani cell wall. Cloned unidirectionally, 5' end of the cDNA cloned into SalI / NotI site of pSPORT 1. Primer: Oligo (dT). Average insert size: 1 kb; Superscript Plasmid system -5' adaptor sequence: 5'CCCACGCGTCCG3' -3' adaptor sequence: 5'GGCCGCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3

text: Mycelium

text: E. coli DH5a cells (ampicillin resistant)

characteristic: lab-host

text: Trichoderma lixii

characteristic: strain

text: TFS (Hypocrea lixii strain ALL-42 mycelium)

text: plasmid


characteristic: External Id

text: School of Basic Medical Science, Wuhan University

characteristic: INSDC center name

text: live

text: Generic

text: Total RNA was isolated from uninfected and Schistosoma japonicum-infected O. hupensis head-foot using Trizol reagent (Invitrogen) following the manusfacturer's protocol. The cDNA synthesis was done using the Super SMART PCR cDNA synthesis kit (Clontech). The cDNA was then directly used for SSH library construction by using the PCR-Select Suppressive Subtraction Hybridization kit (Clontech). Enriched differentially expressed genes, was sequenced using M13F. Adaptor primer sequences were removed from sequence.

text: Oncomelania hupensis

text: head-foot

text: Oncomelania hupensis head-foot SSH library

text: PCR 2.1-TOPO


text: Kyungpook National University School of Medicine

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Plasmodium berghei mixed blood stage library, Clontech (cat. 634901)

text: mixed erytrocytic stage

text: Plasmodium berghei

text: ANKA

text: Plasmodium berghei strain ANKA mixed blood stage library


text: Genoscope - Centre National de Sequencage

text: live

characteristic: INSDC status

text: Generic

text: adult

text: Scyliorhinus canicula

text: brain

text: Scyliorhinus canicula adult brain library (Genoscope - CEA)


text: live

text: Generic

characteristic: NCBI submission model

text: embryonic, stages 19-24

text: Scyliorhinus canicula

text: Scyliorhinus canicula embryonic, stages 19-24 library (Genoscope - CEA)


text: live

text: Generic

characteristic: NCBI submission package

text: embryonic, stages 19-25

text: Scyliorhinus canicula

text: Scyliorhinus canicula embryonic, stages 19-25 library (Genoscope - CEA)


text: live

text: Generic

text: embryonic, stages 9-15

text: Scyliorhinus canicula

text: Scyliorhinus canicula embryonic, stages 9-15 library (Genoscope - CEA)


text: live

text: Generic

text: juvenile (5 days post-hatching)

text: Scyliorhinus canicula

text: Scyliorhinus canicula juvenile library (Genoscope - CEA)


text: Duke University

text: live

text: Generic

text: Total RNAs from brain of normal human were extracted and transcribed into cDNA. The transcripts were amplified by PCR. The amplification products were gel purified, cloned into pGEM-T Easy Vector (Promega), and inserts were sequenced.

text: age 1 to 80

text: brain

characteristic: organ

text: Homo sapiens

text: whole brain

text: Human brain, variant transcript of SHANK3


text: Xinjiang University

text: live

text: Generic

text: Leaves of control plants and salt-treated plants (600 mM NaCl) were collected seperately to build the forward subtractive cDNA library of Halostachys caspica by using the suppression subtractive hyvridization (SSH) method

text: seedling stage

text: Halostachys caspica

text: hermaphrodite

characteristic: sex

text: leaves

text: Differentially expressed genes involved in salt stress response of Halostachys caspica


text: Australian Institute of Marine Science

text: live

text: Generic

text: Rhopaloeides odorabile

text: Heat stressed Rhopaloeides odorabile Library


text: China West Normal University

text: live

text: Generic

text: Three pistils

characteristic: cultivar

text: The subsidiary pistil primordium differentiative phase

text: young spike

text: Triticum aestivum

text: Annealing control primer system library of T. aestivum Three pistils

text: pGEM-T


text: Jiangxi Agriculture University

text: live

text: Generic

text: inbred line

text: Two rice inbred lines, a heat-sensitive one and heat-tolearance one, were selected and treated by high temperature for 3, 6, 9, 24, 72 and 120 hours respectively at the early grain-filling stage (10 days after anthesis). Differentially expressed genes of rice caryopsis were identified with cDNA-Amplified Fragment Length Polymorphism (cDNA-AFLP) technology.

characteristic: description

text: early grain milky stage

characteristic: dev-stage

text: Caryopsis

text: Oryza sativa Japonica Group

text: EcoRI

text: SacI

text: Japonica

text: Rice caryopsis day 8 after anthesis Library

text: Cosmid

text: pGEM-T


text: Universidade de Sao Paulo - USP

text: live

text: Generic

text: Primers targeting the non-structural protein 5 (NS5) from Flaviviridae virus were designed for detection in tick cDNA samples.

text: unfed larvae

text: Rhipicephalus microplus

text: undertemined

text: cDNA from unfed larvae whole tick extract (NS5)

characteristic: title


text: Universidade de Sao Paulo - USP

text: live

text: Generic

text: adult male

text: Rhipicephalus microplus

characteristic: organism

text: male

text: cDNA from adult male whole tick extract (NS5)


text: live

text: Generic

text: adult female

text: Rhipicephalus microplus

text: female

text: cDNA from adult female whole tick extract


text: Universidade de Sao Paulo - USP

text: live

text: Generic

text: Primers targeting the non-structural protein 3 (NS3) from Flaviviridae virus were designed for detection in tick cDNA samples.

text: adult female

text: Rhipicephalus microplus

text: female

text: cDNA from adult female whole tick extract (NS3)


text: Universidade de Sao Paulo - USP

text: live

text: Generic

text: unfed larvae

text: Rhipicephalus microplus

text: undertemined

text: cDNA from unfed larvae whole tick extract (NS3)


text: Universidade de Sao Paulo - USP

text: live

text: Generic

text: adult male

text: Rhipicephalus microplus

text: male

text: cDNA from adult male whole tick extract (NS3)


text: Universidade de Sao Paulo - USP

text: live

text: Generic

text: adult female

text: Rhipicephalus microplus

text: female

text: cDNA from adult female whole tick extract (NS5)


text: live

text: Generic

text: Petunia x hybrida

text: roots

characteristic: tissue-type

text: normalized cDNA library of roots from Petunia FT x hybrida, Mitchell diploid (W115)


text: School of Basic Medical Science, Wuhan University

text: live

text: Generic

text: Total RNA was isolated from Mother sporocysts of Schistosoma japonicum cultured with and without leachate from head-foot of Oncomelania hupensis using Trizol reagent (Invitrogen) following the manusfacturer's protocol. The cDNA synthesis was done using the Super SMART PCR cDNA synthesis kit (Clontech). The cDNA was then directly used for SSH library construction by using the PCR-Select Suppressive Subtraction Hybridization kit (Clontech). Enriched differentially expressed genes, was sequenced using M13F. Adaptor primer sequences were removed from sequence.

text: Schistosoma japonicum

text: Whole body

text: Mother sporocysts of Schistosoma japonicum SSH library

characteristic: vector


text: USDA-Agricultural Research Station

text: live

text: Generic

text: Lactuca sativa

text: Leaf

text: Lactuca sativa PI251246 leaf reverse SSH library


text: Department of Chemistry, Biochemistry B

text: live

text: Generic

text: mycelia cells

characteristic: cell type

text: obtained from sample induced with paraquat; rice blast fungus grown under conditions of oxidative stress

text: exponential phase

text: E.coli

characteristic: lab-host

text: Pyricularia grisea

text: MA 829 (Cooke)

characteristic: strain

text: mycelium

text: Magnaporthe grisea oxidative stress library

text: Plasmid

text: pCR-Blunt II-TOPO


characteristic: External Id

text: Central South University of Forest and Technology

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: Wild

text: Krascheninnikovia lanata (Ceratoides lanata) were treated in low-temperature biochemical incubator for cold stress under the special program: the temperature in incubator decreased by 5C every two hours from room temperature to -15C, then continued treatment at this constant temperature. The coldstressed leaves of K. lanata were collected, and then stored at -75C. The total RNA of the samples was extracted by Total RNA Purification Kit (Invitrogen, USA), and mRNA was purified by mRNA Purification Kit (Amersham Pharmacia Biotech, USA). The cold-stressed normalized full-length cDNA library of K.lanata was constructed by the method of DSN (duplex-specific nuclease)-based normalization and SMART (switching mechanism at 5' end of RNA transcript) full-length cDNA construction. The cDNAs of normalized full-length cDNA library of K.lanata were randomly sequenced by 3730xl DNA analyzer.

text: Krascheninnikovia lanata

text: Sfi I (A)

text: Sfi I (B)

text: leaf

text: Krascheninnikovia lanata cold-stressed cDNA Library

text: Plasmid

text: pDNR-LIB


text: Universidad Andres Bello

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Talaromyces purpureogenus

text: Penicillium purpurogenum Differential Expression Library


text: Korea Polar Research Institute

text: live

text: Generic

characteristic: NCBI submission package

text: middle of exponential

text: Pyramimonas gelidicola

text: whole body

text: P. gelidicola cDNA library at treatment condition (minus 4 degree)


text: Kyushu University

text: live

text: Generic

text: adult

text: Carassius langsdorfii

text: spleen and kidney

text: cDNA library from CHNV (crucian carp haematopoietic necrosis virus)-infected ginbuna crucian carp


text: Korea Polar Research Institute

text: live

text: Generic

text: middle of exponential

text: Pyramimonas gelidicola

text: whole body

text: P. gelidicola cDNA library at control condition (4 degree)


text: Wayne State University

text: live

text: Generic

text: Homo sapiens

text: Invasive ductal carcinoma of the breast

text: Human T7 cDNA library of breast cancer proteins


text: Rutgers University Department of Ecology, Evolution, and Natural Resources, and Institute of Marine and Coastal Sciences

text: live

text: Generic

text: The cDNAs were generated from 2 microgram of total RNA using MINT cDNA Synthesis Kit (Evrogen, Russia), normalized using TRIMMER cDNA Normalization Kit (Evrogen, Russia), and sequenced using 454 pyrosequencing technology at the University of Iowa.

text: Porphyridium purpureum

text: Porphyridium cruentum CCMP1328 cDNA library


text: University of Bern

text: live

text: Generic

text: Petunia axillaris subsp. axillaris

text: corolla tubes

text: normalized cDNA library of corolla tubes (region D1 and D2)


text: live

text: Generic

text: UNILIB:50472; RNA of roots treated with 5 mM KH2PO4 was substracted from RNA of control roots

text: Petunia x hybrida

text: roots

text: substractive cDNA library of Petunia hybrida, Mitchell diploid (W115)


text: University of Bern

text: live

text: Generic

text: Petunia x hybrida

text: roots

text: normalized cDNA library of roots from Petunia x hybrida, Mitchell diploid (W115)


text: Dalian Ocean University

text: live

text: Generic

text: Library constrcted with SMART cDNA Library Construction Kit

text: E. coli XL1-Blue, E. coli BM25.8

text: Tubefoot

characteristic: organ

text: Apostichopus japonicus

text: SifIA

text: SifIB

text: Tubefoot

text: Sea Cucumber full-length cDNA library from tubefoot

text: Phage

text: Lambada TripleX2


text: Faculty of Medicine-Ramathibodi Hospital, Mahidol University

text: live

text: Generic

text: mycelium

text: Pythium insidiosum

text: PinsEST Pythium insidiosum CBS 119452 mycelium library


text: University of British Columbia

text: live

text: Generic

text: Lavender lady

characteristic: cultivar

text: 30% flower

text: E. coli

text: flower

text: Lavandula angustifolia

text: EcoRI

text: XhoI

text: Phagemid

text: pBluescript II SK (+)


text: University of British Columbia

text: live

text: Generic

text: leaf

text: Lavandula angustifolia


text: National Institute of Allergy and Infectious Diseases

text: live

text: Generic

text: Unidirectional library was constructed using long distance PCR (Smart cDNA kit) and Lambda TriplEx2 vector

characteristic: description

text: nymph

characteristic: dev-stage

text: salivary glands

text: Amblyomma rotundatum

text: Nymphal salivary gland cDNA library Amb_rotSG1

text: Phage

text: Lambda TriplEx2


text: Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria

text: live

text: Generic

text: Heterokaryotic hyphae

text: A vegetatively incompatible interaction (tester) was compared with a no pairing reaction (i.e., a heterokaryon that is not interacting with itself or another heterokaryon)(driver). SSH was followed by pyrosequencing.

characteristic: isolate

text: Amylostereum areolatum

characteristic: organism

text: Mycelium

text: Amylostereum areolatum CMW31512 vegetative incompatibility SSH library

characteristic: title


text: Universidade de Sao Paulo

text: live

text: Generic

text: larval ovary

text: Apis mellifera

text: female

characteristic: sex

text: RDA worker L5F3 ovary Apis mellifera

text: Plasmid

text: pGEM

characteristic: vector


text: Universidade de Sao Paulo

text: live

text: Generic

text: larval ovary

text: Apis mellifera

text: female

text: RDA queen L5F3 ovary Apis mellifera

text: Plasmid

text: pGEM


text: Nanfan Hospital

text: live

text: Generic

text: Listonella anguillarum infected ayu revealed by suppression subtractive hybridization library

text: juvenile

text: Plecoglossus altivelis

text: Listonella anguillarum infected ayu SSH Library


text: Washington and Lee University

text: live

text: Generic

text: adult females

text: Steatoda grossa

text: female

text: Spider Pharm, Yarnell, AZ

characteristic: strain

text: silk glands

characteristic: tissue-type

text: Steatoda grossa silk gland cDNA library

text: plasmid


characteristic: External Id

text: University of Hawaii at Manoa

characteristic: INSDC center name

text: live

text: Generic

text: Library was prepared by performing interspecies suppression subtractive hybridization using cDNAs of L. leucocephala as the tester and cDNAs of Acacia confusa as the driver

text: Leucaena leucocephala

text: Young shoots

text: interspecies suppression subtractive hybridization library of Leucaena leucocephala


text: Henan Agricultural University

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: root

characteristic: cell type

text: To study molecular mechanism underline growth and development of Rehmannia glutinosa, SSH was used to screen key genes related to the root development. The cDNA library was constructed by taking the cDNA of Rehmannia glutinosa roots as the tester and that of Rehmannia glutinosa leaves as the driver.

text: the tuberous root expansion stage

text: Rehmannia glutinosa

text: root cDNA library of Rehmannia glutinosa L.

text: pMD18-T (TaKaRa)


text: University of Bern

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: UNILIB:50472; RNA of control roots was substracted from RNA of mycorrhizal roots (Glomus intraradices)

text: Petunia x hybrida

text: roots

text: substractive cDNA library of Petunia hybrida, Mitchell diploid (W115)(EB-4)


text: University of Bern

text: live

text: Generic

text: UNILIB:50472; RNA of roots treated with 5 mM KH2PO4 was substracted from RNA of control roots.

characteristic: description

text: Petunia x hybrida

text: roots

text: substractive cDNA library of Petunia hybrida, Mitchell diploid (W115)(EB-3)


text: University of Bern

text: live

text: Generic

text: UNILIB:50472; RNA of mycorrhizal roots (Glomus intraradices) was substracted from RNA of control roots

text: Petunia x hybrida

text: roots

text: substractive cDNA library of Petunia hybrida, Mitchell diploid (W115)(EB-2)


text: University of Bern

text: live

text: Generic

text: UNILIB:50472; RNA of control roots was substracted from RNA of roots treated with 5 mM KH2PO4

text: Petunia x hybrida

text: roots

text: substractive cDNA library of Petunia hybrida, Mitchell diploid (W115)(EB-1)


text: Institute for Molecular Bioscience, University of Queensland

text: live

text: Generic

text: subtraction library using a post abdomen derived mRNA

text: Hottentotta judaicus

text: telson

text: scorpion replete venom gland library


text: University of Maine

text: live

text: Generic

text: Jasmine 85

characteristic: cultivar

text: Detached leaf segments were inoculated with a PDA plug of 3 day old R. solani culture or plain PDA plug without mycelia. Samples utilized for SSH library construction (inoculated and non-inoculated) were harvested at 16 hpi. Total RNA was isolated following the RNeasy Plant Mini Kit protocol (Qiagen Inc., Valencia, CA, USA) and cDNA was synthesized from 100 ng of total RNA with the Super SMARTTM PCR cDNA synthesis kit (Clontech, Palo Alto, CA, USA). cDNA from inoculated and control samples was used for PCR-SelectTM cDNA subtraction (Clontech, Palo Alto, CA, USA)). The PCR mixture enriched for differentially expressed cDNAs was cloned into the TOPO TA cloning vector (Invitrogen, Carlsbad, CA) and sequenced using ABI-PRISM BigDyeTM Terminator Cycle Sequencing (Applied Biosystems, ABI 3700).

text: Growth stage V5; second youngest leaves

characteristic: dev-stage

text: Oryza sativa Japonica Group

text: Leaf

text: Rice infected with Rhizoctonia solani AG1 SSH cDNA library

text: TOPO TA


text: University of Oslo

text: live

text: Generic

text: Collected from seawater in Skagerrak, Norway on March 29, 2001

text: Pseudochattonella farcimen

characteristic: organism

text: Pseudochattonella farcimen UIO109 library


text: Max Planck Institute for Plant Breeding Research

text: live

text: Generic

text: primary hyphae

text: biotrophic infection

text: Colletotrichum higginsianum

text: Colletotrichum higginsianum IMI349063A biotrophic infection

characteristic: title


text: NIGEB

text: live

text: Generic

text: Infectious bursal disease virus

text: Infectious bursal disease virus library (Sedighzadeh S)


text: Faculty of Biology, Plant Physiology

text: live

text: Generic

text: Seeds of Lepidium sativum (garden cress) were imbibed in 10% MS-medium at 18 degrees and continuous light for 12 hours, respectively. cDNA synthesised from RNA extracted from whole seeds was used as template for PCR ampflification. The obtained cDNA fragments were cloned in pTOPO.

text: imbibed seed

text: E.coli TOP10

characteristic: lab-host

text: whole seeds

characteristic: organ

text: Lepidium sativum

text: Lepidium sativum Fr14 12h imbibed seeds library


text: University of KwaZulu-Natal

text: live

text: Generic

text: Total RNA extracted with tri-reagent, differential display performed (Genhunter) with 32 primer combinations, bands reamplified and cloned into PCR-TRAP cloning system, colony PCR performed and sequenced

text: Juvenile

text: Haliotis midae

text: Muscle

text: Haliotis midae juvenile muscle DDRT-PCR

text: Plasmid

text: PCR-TRAP

characteristic: vector


text: College of Life Science and Technology, Xinjiang University

text: live

text: Generic

text: Suppression subtractive hybridization (SSH) library of the halophyte Karelinia caspica under salt stress

text: leaf

text: Karelinia caspia

text: SSH library of Karelinia caspica under salt stress


text: Anhui Agricultural University

text: live

text: Generic

text: YJ63X and YJ63Y

characteristic: cell-line

text: Camellia sinensis

text: hermaphrodite

characteristic: sex

text: callus

characteristic: tissue-type

text: cDNA-AFLP of tea (Camellia sinensis L.) callues with different catechins contents

text: pMD18-T Simple


characteristic: External Id

text: Northeast Forestry University

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: Larix gmelinii

text: Needles

text: cDNA Library of Larix gmelini under treated with Jasmonic acid


text: Universitat de Barcelona

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Adult cells and tissues primed with bacterial stimuli

text: Sparus aurata

text: mixed

text: Head kidney, spleen, leukocytes

text: Seabream ZAP Express immune library bacterial stimuli

text: Phage

text: pBK-CMV


text: Universitat de Barcelona

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Adult tissues from fish primed with various viral stimuli

text: Sparus aurata

text: mixed

text: Head kidney, spleen

text: Seabream ZAP Express immune library viral stimuli

text: Phage

text: pBK-CMV


text: Waksman Institute, Rutgers University

text: live

text: Generic

text: Wolffia australiana cDNAs were reversed transcribed from polyA-selected mRNAs. SfiIA and SfiIB linkers were added to the cDNAs via the primers used to synthesize first and second strand cDNAs. The cDNAs were cut with SfiI to generate SfiI sticky ends, and the cDNAs were directionally ligated into SfiIA and SfiIB-cut plasmid vector pTriplEX2. Recombinant plasmids were transformed into E. coli strain DH5a.

characteristic: description

text: Mixed stages

text: E. coli DH5a

text: Whole plant

text: Wolffia australiana

text: SfiI

text: WSSP Wolffia australiana cDNA library

text: Plasmid


text: Huazhong Agricultural University

text: live

text: Generic

text: Potato tubers of wild species Solanum Berthaultii (S. Berthaultii) were propagated in the Potato Germplasm Resources Garden of the University and recovered after maturation. The new harvest potato tubers were stored at 20degC for 10 days before experiments. For the forward subtracted library construction, mRNAs from S. berthaultii stored at 20degC for 5 days and mRNAs from S. Berthaultii stored at 4degC for 5 days were used as driver and tester, respectively. For the reverse subtracted library, mRNAs from S. Berthaultii stored at 4degC for 5 days and mRNAs from S. Berthaultii stored at 20degC for 5 days were used as driver and tester, respectively. Total RNA samples were prepared from potato tubers by Trizol reagents (Sigma, USA), mRNA was purified from total RNA using an Oligotex mRNA isolation column under the manufacturer's guidance (Qiangen, Germany). SSH library was performed using PCR Select cDNA Subtraction kit (Clontech, USA) according to the manufacturer's instructions. The enriched specific PCR product was purified under the instruction of a DNA recovery kit (Takara, China) and restricted by SmaI/NotI, ligated into plasmid pBluescript SK (-), then transformed into Escherichia coli DH5alpha. Single white colonies growing on LB agar containing Amp/X-gal/IPTG were propagated in the 384-well plates (Greiner, Germany), clones were primarily screened by PCR with primers specific for plasmid pBluescript SK (-) (T3 and T7 primers), clones with single amplicon were added with glycerol to 15% (v/v) and stored at -70degC.

text: Adult

characteristic: dev-stage

text: Escherichia coli DH5alpha

text: Solanum berthaultii

text: Tubers

text: potato tubers cold stress substracted cDNA library

text: pBluescripte SK (-)


text: INRA - UMR BIOGECO

text: live

text: Generic

text: 1 year old seedling stems

text: Pinus radiata

characteristic: organism

text: stem

text: Pinus radiata stem 1 year old seedling stems


text: Department of Animal Science and Technology, Northwest A&F University

text: live

text: Generic

text: SSH library was constructed, and sequences from library constructed by sample with low fecundity as tester and high fecundity as driver were labeled as RL

text: 24 hours after estrus (in the middle of estrus)

text: Ovary

text: Capra hircus

text: Female

text: Guanzhong

characteristic: strain

text: Ovary

text: ovary SSH RL library of Dairy goat in estrus phase

characteristic: title

text: PMD 19-T


text: live

text: Generic

text: SSH library was constructed, and sequences from library constructed by sample with high fecundity as tester and low fecundity as driver were labeled as PL

text: Ovary

characteristic: organ

text: Capra hircus

text: Female

text: Guanzhong

text: Ovary

text: ovary SSH PL library of Dairy goat in estrus phase

text: PMD 19-T


text: Rubber Research Institute of Malaysia

text: live

text: Generic

characteristic: cultivar

text: Total RNA was isolated from latex of field-grown rubber trees. Following preparation of latex mRNA, subsequent steps of cDNA synthesis and library construction were performed using the ZAP-cDNA Synthesis Kit and ZAP-cDNA Gigapack II Gold Cloning Kit (Stratagene). Latex cDNAs cloned in the Lambda Uni-Zap XR vector were amplified once from the packaging reaction. Subsequently, Lambda Uni-Zap XR clones were in vivo excised into pBluescript phagemids for use as sequencing templates.

text: Hevea brasiliensis

text: EcoRI

text: XhoI

text: Latex

text: Hevea brasiliensis YLX600 library

text: Phage

text: Lambda UniZAP XR

characteristic: vector


text: Ministry of Agriculture

text: live

text: Generic

text: Elaeis guineensis

text: 17 week fruit mesocarp

text: Elaeis guineensis 17 week fruit mesocarp


text: Korea National Institute of Health

text: live

text: Generic

text: adult

text: Clonorchis sinensis

text: Clonorchis sinensis adult


text: Korea National Institute of Health

text: live

text: Generic

text: metacercaria

text: Clonorchis sinensis

text: Clonorchis sinensis metacercaria


text: Korea National Institute of Health

text: live

text: Generic

text: egg

text: Clonorchis sinensis

text: Clonorchis sinensis egg


text: Sugarcane Research Institute, Fujian Agriculture and Forestry University

text: live

text: Generic

text: NCo 376

text: In this study, with smut high-resistant sugarcane variety of NCo376 as plant material, cDNA-AFLP along with silver staining were occupied to study differential gene expression during sugarcane-U.scitaminea interaction.

text: Saccharum hybrid cultivar NCo 376

text: bud

text: Sugarcane variety NCo 376 ESTs from cDNA-AFLP analysis


text: Research Institute of Subtropical Forestry, Chinese Academy of Forestry

text: live

text: Generic

text: Milliongolds

text: E. coli DH5alpha

characteristic: lab-host

text: flower buds

text: Oncidium hybrid cultivar

text: hermaphrodite

characteristic: sex

text: Oncidium hybrid cultivar Milliongolds flower development cDNA-AFLP

text: pGEM-T Vector


characteristic: External Id

text: Institute of Himalyan Bioresource Technology (CSIR)

text: live

text: Generic

text: Arnebia euchroma

text: Root

characteristic: tissue-type

text: Differentially expressed ESTs from roots of Arnebia euchroma (Royle) Johnston

text: pGEM-T Vector (Promega)


text: Agrilife Research and Extension Center

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: TSP05-4 (Drought tolerant)

text: formative phase

text: leaf

text: Saccharum hybrid cultivar TSP05-4

text: leaf

text: Water-stressed sugarcane cDNA-AFLP fragments (Saccharum hybrid cultivar TSP05-4)


text: Agrilife Research and Extension Center

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: TCP02-4589 (Drought sensitive)

text: formative phase

text: leaf

text: Saccharum hybrid cultivar TCP02-4589

text: leaf

text: Water-stressed sugarcane cDNA-AFLP fragments (Saccharum hybrid cultivar TCP02-4589)


text: North Carolina Central University

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: 2-week old after germination

text: Astragalus racemosus

text: whole seedlings

text: Astragalus racemosus 2-week old seedlings grown in 20uM potassium selenate library


text: NASA Ames Research Center

text: live

text: Generic

text: Total RNA was isolated from P. waltl larva with TRIzol reagent. cDNA library was constructed using CloneMiner cDNA library construction kit (Invitrogen) according to the manufacturer's instructions. Double stranded cDNA was directionally cloned into pDONR222 vector using Gateway technology (Invitrogen) and transformed into E. coli DH10B T1 Phage Resistant cells. Extracted plasmid DNA was subjected to a single pass sequencing from M13 Forward universal primer site (5' end) at the Sequetech Corporation (Mountain View, CA).

characteristic: description

text: larval

characteristic: dev-stage

text: Pleurodeles waltl

text: whole embryo (larva)

text: Pleurodeles waltl larval cDNA library (LRV)

text: plasmid


text: Lab. Biologia Molecular, Divisao de Ciencias Biomedicas, Diretoria de Pesquisa e Desenvolvimento

text: live

text: Generic

text: Ten adult specimens of Phoneutria nigriventer from Minas Gerais State maintained at the Fundacao Ezequiel Dias in Belo Horizonte - Brazil, were milked two days before venom glands dissection, which were immediately frozen in liquid nitrogen. Total RNA was extracted with TRIzol reagent (Invitrogen, USA).The mRNA purification was performed on an oligo(dT)- cellulose affinity column using the mRNA Purification Kit (Pharmacia, Sweden). A cDNA library was constructed following the instructions of the Super Script Plasmid System with Gateway Technology for cDNA Synthesis and Cloning Kit (Invitrogen). Selected sized cDNA fragments ranged from 300 to 800 pb separated by agarose gel electrophoresis were cloned into the psPORT 1 plasmids (Invitrogen) with the following adapters: Not I: 5' GACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15 3' and Sal I: 5' TCGACCCACGCGTCCG 3'. The recombinant plasmids were transformed into Escherichia coli DH5-alpha (Invitrogen). Random colonies were selected and cultured in Circle Grown (MP Biomedicals, USA) medium containing ampicilin (100 ug/ml). After overnight culture, the plasmids were extracted by alkaline lysis method (Sambrook et al, 1989) and single-passed sequenced on an ABI 3130 sequencer using the standard M13 reverse primer and Big Dye terminator v3.1 Cycle sequencing kit (Applied Biosystems, USA).

text: Venom gland

text: Phoneutria nigriventer

characteristic: organism

text: Not I

text: Sal I

text: Phoneutria nigriventer venom gland cDNA library

text: plasmid


text: Institute of Hydrobiology, Chinese Academy of Sciences

text: live

text: Generic

text: Cyprinus carpio

text: Female

text: Hypothalamus, pituitary, and ovary libraty of female sGnRH-antisense transgenic common carp

characteristic: title

text: Plasmid


text: Institute of Himalayan Bioresource Technology

text: live

text: Generic

text: Kangra Jat

text: Camellia sinensis

text: Apical Bud

text: Camellia sinensis winter dormancy differential display of mRNA

text: Plasmid

text: PCR-trap vector


text: United Planters' Association of Southern India-Tea Research Foundation, Tea Research Institute

text: live

text: Generic

text: UPASI 10

text: Creator SMART cDNA library Construction kit (Clonetech)

text: Camellia sinensis

text: leaves

text: cDNA library from Pestalotiopsis sp. infected leaves of Camellia sinensis (U10GBcDNA)

text: Plasmid


text: Federal University of Rio Grande do Sul

text: live

text: Generic

text: sequences isolated from leaves of P. brachyceras UV-B radiation exposed, obtained from Suppressive Subtractive Hybridization, cloned into pCR2.1-TOPO Vector, transformed into Escherichia coli XL1 Blue eletrocompetent cells, and then sequenced.

text: Psychotria brachyceras

text: leaf

text: SSH Library from leaves of Psychotria brachyceras UV-B exposed


text: Federal University of Rio Grande do Sul

text: live

text: Generic

text: sequences isolated from leaves of P. brachyceras, obtained from RT-PCR, purified, cloned into pCR2.1-TOPO Vector, transformed into Escherichia coli XL1 Blue eletrocompetent cells, and then sequenced.

text: Psychotria brachyceras

text: leaf

text: Psychotria brachyceras reference genes library


text: National Research Council of Canada

text: live

text: Generic

text: Bethune

text: Globular

text: Embryo

characteristic: organ

text: Linum usitatissimum


text: National Research Council of Canada

text: live

text: Generic

text: Bethune

characteristic: cultivar

text: Heart

text: Embryo

text: Linum usitatissimum


text: National Research Council of Canada

text: live

text: Generic

text: Bethune

text: Torpedo

text: Embryo

text: Linum usitatissimum


text: National Research Council of Canada

text: live

text: Generic

text: Bethune

text: Cotyledon

text: Embryo

text: Linum usitatissimum


text: National Research Council of Canada

text: live

text: Generic

text: Bethune

text: Mature

text: Embryo

text: Linum usitatissimum


text: National Research Council of Canada

text: live

text: Generic

text: Bethune

text: Globular

text: Seed Coat

text: Linum usitatissimum


text: National Research Council of Canada

text: live

text: Generic

text: Bethune

text: Torpedo

text: Seed Coat

text: Linum usitatissimum


text: National Research Council of Canada

text: live

text: Generic

text: Bethune

text: Endosperm

text: Linum usitatissimum


text: National Research Council of Canada

text: live

text: Generic

text: Bethune

text: Flower

text: Linum usitatissimum


text: National Research Council of Canada

text: live

text: Generic

text: Bethune

text: Etiolated Seedling

text: Linum usitatissimum


text: National Research Council of Canada

text: live

text: Generic

text: Bethune

text: Leaf

text: Linum usitatissimum


text: National Research Council of Canada

text: live

text: Generic

text: Bethune

text: Stem

text: Linum usitatissimum


text: National Research Council of Canada

text: live

text: Generic

text: Norlin

text: Peeled stem

text: Linum usitatissimum


text: NRC on Plant Biotechnology, IARI

text: live

text: Generic

text: Suppression subtracted cDNA library was constructed using cDNA synthesized from Fusarium oxysporum challenged WR-315 as tester and that from control as driver using Clontech PCR-Select cDNA subtraction Kit (CLONTECH, USA) according to the manufacturer's protocol. Differentially expressed cDNAs were cloned in pGEMT-Easy vector and transformed in E. Coli. Sanger sequencing of the selected clones were done with universal sequencing primers T7, SP6 and M13.

text: seedling

text: Cicer arietinum

text: shoot

text: Suppression subtracted cDNA library TSA1 of fusarium wilt stressed Cicer arietinum cDNA

text: pGEM-T Easy vector

characteristic: vector


text: NRC on Plant Biotechnology, IARI

text: live

text: Generic

text: WR-315; BG-256

text: Suppression subtracted cDNA library was constructed using cDNA synthesized from Fusarium oxysporum challenged WR-315 as tester and that from Fusarium oxysporum challenged BG-256 as driver using Clontech PCR-Select cDNA subtraction Kit (CLONTECH, USA) according to the manufacturer's protocol. Differentially expressed cDNAs were cloned in pGEMT-Easy vector and transformed in E.Coli. Sanger sequencing of the selected clones were done with universal sequencing primers T7, SP6 and M13.

text: seedling

text: Cicer arietinum

text: shoot

text: Suppression subtracted cDNA library TSB1 of fusarium wilt stressed Cicer arietinum cDNA

text: pGEM-T Easy vector


text: University of Saskatchewan

text: live

text: Generic

text: The cDNA library was prepared from leaftlet of canadian lentil cultivar 'Eston' inoculated with Colletotrichum truncatum CT-21.

text: Hemibiotrophic switch

text: Laboratory host

characteristic: lab-host

text: Lens culinaris/Colletotrichum truncatum mixed EST library

text: Lentil defense genes induced during hemibiotrophic infection (Stratagene, cat. #200455)

text: Plasmid

text: pBluescript II SK(+) XR


text: Berry College

text: live

text: Generic

text: Expression induced by 4 hr exposure to 50 ug/L of Permethrin

text: Adult

text: Acropora cervicornis

text: Coral stress responses to permethrin


text: Berry College

text: live

text: Generic

text: Adult

text: Acropora cervicornis

text: Coral stress responses to copper


text: National Center of Excellence in Molecular Biology

text: live

text: Generic

text: After twenty-four hours of salt stress

text: leaf

text: Gossypium arboreum

text: Hermaphrodite

characteristic: sex

text: Total leaf tissue

text: Differential display of COTTON GENOTYPE, leaf, after 24 hours of salt stress

text: Plasmid


characteristic: External Id

text: United Planters' Association of Southern India-Tea Research Foundation, Tea Research Institute

text: live

text: Generic

text: Messenger RNA was extracted from healthy and Exobasidium vexans infected tea leaf tissue and subtracted cDNA library was constructed following SSH method (Clonetech)

text: Harvesting stage

text: E.coli JM109

text: Camellia sinensis

text: Leaf

text: reverse SSH library of leaf of Camellia sinensis cultivar SA6

text: Plasmid


text: College of Horticulture, Northwest A&F University

text: live

text: Generic

text: seedling (growing stage)

text: E.coli BM25.8

text: Vitis amurensis

text: SfiI

text: Heilongjiang

characteristic: strain

text: Young leaf

characteristic: tissue-type

text: cDNA library of Chinese wild Vitis amurensis cold-stressed young leaves from potted plant

text: Phage

text: Lambda TripLEx2


text: Universidade Estadual de Santa Cruz

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: Suppressive Subtractive Hybridization library (PCR-Select cDNA Subtraction kit; Clontech)

text: Leaf

text: Hevea brasiliensis

text: Hevea brasiliensis cultivar MDF180 leaves (6 to 72 hours-post-inoculation by Microcyclus ulei)

text: Plasmid

text: pGEM-T


text: Universidade Estadual de Santa Cruz

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Leaf

text: Hevea brasiliensis

text: Hevea brasiliensis cultivar MDF180 leaves (4 to 28 days-post-inoculation by Microcyclus ulei)

text: Plasmid

text: pGEM-T


text: Universidade Estadual de Santa Cruz

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Leaf

text: Hevea brasiliensis

text: Hevea brasiliensis cultivar PB314 leaves (6 to 72 hours-post-inoculation by Microcyclus ulei)

text: Plasmid

text: pGEM-T


text: Universidade Estadual de Santa Cruz

text: live

text: Generic

characteristic: description

text: Leaf

text: Hevea brasiliensis

text: Hevea brasiliensis cultivar PB314 leaves (4 to 28 days-post-inoculation by Microcyclus ulei)

text: Plasmid

text: pGEM-T


text: Universidade Estadual de Santa Cruz

text: live

text: Generic

text: Leaf

text: Hevea brasiliensis

characteristic: organism

text: Hevea brasiliensis cultivar PB314 leaves (34 to 58 days-post-inoculation by Microcyclus ulei)

text: Plasmid

text: pGEM-T


text: Chungbuk National University

text: live

text: Generic

text: Stratagene's cDNA Synthesis Kit (catalog number 200401), ZAP Express Vector Kit (catalog number 239211), and Gigapack III Gold Packaging Extract (catalog number 200201) were used to construct the cDNA library. After second-strand cDNA synthesis, adapter ligation, and size fractionation, the column eluent was then ligated into Stratagene's UniZAP XR vector that has been digested with EcoRI and XhoI, and phosphorylated by Stratagene. The ligated DNA was packaged using Stratagene's Gigapack III Gold Packaging Extract and the pBluescript SK phagemid and insert was circularized to phagemid by in vivo excision.

text: Phyllostachys edulis

text: EcoRI

text: XhoI

text: fast growing shoot

text: Phyllostachys edulis fast growing shoot cDNA library

characteristic: title

text: pBluescript SK


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT) primed cDNA and cloned into the pFLC-I vector. PolyA RNA was primed with an oligo dT primer (5'-AAGCAGTGGTATCAACGCAGAGTGGCCCTTATGGCCTTTTTTTTTTTTTTTTVN-3') and cloned into the pFLC-I vector. Range of insert sizes: 1-6 kb. The library was constructed in the laboratory of A. Gracey (University of Southern California) and sequencing performed at the DOE Joint Genome Institute (Walnut Creek, CA).

text: combined

characteristic: dev-stage

text: Botryllus schlosseri

text: SfiI-A

text: SfiI-B

text: hermaphrodite

text: whole

text: CCAO Botryllus schlosseri total asexual and embryonic development

text: pFLC-I


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT) primed cDNA and cloned into the pExpress-1 vector. PolyA RNA was primed with an oligo dT primer. The library was constructed in the laboratory of A. Gracey (University of Southern California) and sequencing performed at the DOE Joint Genome Institute (Walnut Creek, CA).

text: Botryllus schlosseri

text: EcoRV

text: NotI

text: CCTT Botryllus schlosseri combined normalized


text: DOE Joint Genome Institute

text: live

text: Generic

text: combined

text: Botryllus schlosseri

text: SfiI-A

text: SfiI-B

text: hermaphrodite

text: whole

text: CCTU Botryllus schlosseri total asexual and embronic development subtracted

text: pFLC-I


text: CPCRI

text: live

text: Generic

text: West Coast Tall

characteristic: cultivar

text: To identify and isolate cDNAs representing transcripts differentially expressed during water stress in coconut, in vitro grown coconut plantlets were treated with PEG 6000 to create stress conditions. We have then applied differential display technique to the comparison of PEG 6000-treated and untreated plantlets. Analysis using the 30 primer combinations revealed 114 transcripts to be differentially expressed. The transcript derived fragments (TDFs) were eluted, cloned and sequenced.

text: Two year old embryo cultured plantlets

text: Cocos nucifera

text: Leaflets of water-stressed plantlets

text: Cocos nucifera West Coast Tall differential display cDNA library


text: British Antarctic Survey

text: live

text: Generic

text: Amphiura filiformis

text: Amphiura filiformis regenerating arm

characteristic: vector


text: USDA-ARS

text: live

text: Generic

text: Subtractive hybridization was performed with the PCR-Select cDNA Subtraction Kit (Clontech), according to the manufacture's protocol. Tester tissue consisted of the anterior kidney of fish 12 hours post vaccination with killed Streptoccus iniae ARS-60 vaccine. Driver tissue was the anterior kidney of non-vaccinated fish.

text: 8 months

text: anterior kidney

characteristic: organ

text: Oreochromis niloticus

text: Auburn

text: Nile tilapia anterior kidney subtractive library 12h post vaccination with killed Streptoccus iniae ARS-60 vaccine

text: pGEM T easy (promega)


text: Indian Agricultural Research Institute

text: live

text: Generic

text: Differentially regulated transcripts in incompatible interaction of wheat challenged with Puccinia triticina pathotype 77-5 (syn. 121 R63-1)

text: The flag leaf at adult plant stage

text: Triticum aestivum

text: Leaf

text: Transcript derived fragments (TDFs) from wheat leaves (cultivar CSP44 + Lr48) inoculated by leaf rust fungus

text: pGEM-T easy


text: Edmund Mach Foundation

text: live

text: Generic

text: Teroldego, Merzling (rootstock)

text: Expressed tags library obtained by cDNA-AFLP analysis of leaf samples from Merzling and one F1 individual (F1 21/66) of the Merzling x Teroldego cross upon infection with Plasmopara viticola.

text: fully expanded leaves

text: Leaf

text: Vitis vinifera

text: hermaphrodite

characteristic: sex

text: Grapevine-Plasmopara viticola interact library


characteristic: External Id

text: Zhejiang University

text: live

text: Generic

text: Jinyan No. 4

text: Cucumis sativus

text: Phloem sap

text: Cucumis sativus Jinyan No. 4 suppressive subtractive hybridization (SSH) library

text: pGEM-T easy vector


text: Escola Superior de Biotecnologia da Universidade Catolica Portuguesa

text: live

text: Generic

text: The subtractive hybridization protocol from BD Biosciences Clontech (Palo Alto, CA) was used to generate cDNA libraries from Pinus pinaster inoculated with Bursaphelenchus xylophilus.

text: 2 year-old plant

text: Pinus pinaster

text: Stem

text: cDNA library of Pinus pinaster inoculated with pine wood nematode (PWN)


text: live

text: Generic

text: The subtractive hybridization protocol from BD Biosciences Clontech (Palo Alto, CA) was used to generate cDNA libraries from Pinus pinea inoculated with Bursaphelenchus xylophilus.

text: 2 year-old plant

text: Pinus pinea

text: Stem

text: cDNA library of Pinus pinea inoculated with pine wood nematode (PWN)


text: Div. of Crop Protection, Central Research Institute for Jute and Allied Fibres

characteristic: INSDC secondary accession

text: live

text: Generic

characteristic: SRA accession

text: A SSH library of mesta against MeYVMV infection. Mesta plants were raised from HC-583 seeds under glasshouse condition and whitefly transmission was carried out with 11 days old plants. Total RNA were isolated from collected leaves of infected plants as well as of control plants after 12 hr, 24 hr, 2 and 10 dpi using Trizol reagent (invitrogen). mRNA was isolated by mRNA isolation kit (Roche Applied Science). The differentially regulated subtracted cDNA pool in healthy plants were obtained using the Clontech PCR select Subtraction Kit. This subtracted cDNA pool were then cloned in pJET vector and transformed in DH5-alpha cells and thus differentially regulated cDNA library was constructed.

text: Leaves from 12 days old plants of mesta (HC-583).

text: Escherichia coli DH5a

characteristic: lab-host

text: Hibiscus cannabinus

text: Leaves

text: Reverse subtracted library of healthy Mesta plant against infection with Mesta yellow vein mosaic virus


text: UMR CNRS 6539 Institut Universitaire Europeen de la Mer

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: Suppression Subtractive Hybridization was used to enrich for transcripts either up or down regulated after exposure to a pathogenic bacterium, Vibrio tapetis. The library was prepared using protocol given by supplier (Clontech)

text: Adults

text: Ruditapes philippinarum

text: Rp

characteristic: strain

text: Hemocytes

characteristic: tissue-type

text: Ruditapes philippinarum cDNAs from SSH library

text: Plasmid

text: pGEM-T vector


text: Helmholtz Zentrum Muenchen

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: immune cells

characteristic: cell type

text: adult

text: Equus caballus

text: male

text: blood

text: normalised cDNA library of equine immune cells


text: Chaudhary Charan Singh University

text: live

text: Generic

text: CSP44 + Lr48

characteristic: cultivar

text: Differentially regulated TDFs identified in incompatible interaction of wheat challenged with Puccinia triticina

characteristic: description

text: Post anthesis

characteristic: dev-stage

text: Triticum aestivum

characteristic: organism

text: Flag Leaf

text: Triticum aestivum leaves inoculated by leaf rust fungus

characteristic: title

text: pGEM-T easy


characteristic: External Id

text: Lanzhou University

text: live

text: Generic

text: Cleistogenes songorica

text: roots and leaves

text: Cleistogenes songorica drought stress induced library


text: Max Planck Institute for Chemical Ecology

text: live

text: Generic

text: Gmel L norm; Galleria mellonella larval transcriptome analysis; normalized cDNA library

text: Whole larvae

characteristic: organ

text: Galleria mellonella

text: Mixed

characteristic: sex

text: Mixed

text: Galleria mellonella larval transcriptome analysis normalized cDNA library

text: Plasmid

text: pGEM-Teasy

characteristic: vector


text: Central Salt and Marine Chemicals Research Institute (CSIR)

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: 1-year old plants

text: Jatropha curcas

text: leaf tissue

text: Jatropha curcas 1 year old plant leaf tissue library


text: Chongqing University

text: live

text: Generic

text: To study molecular mechanism underline Spodoptera litura in response to Metarhizium anisopline, Suppression subtractive hybridization (SSH) associated with cDNA array dot bloting was used to screen differentially expressed genes in fat body following parasite challenge.Total RNA from the fat body of spodoptera literal injected with water was used to synthetize cDNA which used as driver (24h after injection),cDNA synthetized by total RNA which was extracted form fat body of spodoptera literal injected with Metarhizium anisopline used as tester 24h after injection).

text: 5th instar larvae

text: Spodoptera litura

text: fat body

characteristic: tissue-type

text: Spodoptera litura SSH cDNA library


text: Universiti Malaysis Sabah

characteristic: INSDC last update

text: live

text: Generic

text: cDNA of the green mature pineapple fruit was constructed using SMART cDNA library construction kit. Normalization of the ds cDNA was performed using duplex-specific nuclease (DSN) before cloned into lambda TripIEX2

text: Mature green

text: Ananas comosus

text: Fruit

text: Ananas comosus green mature fruit cDNA library

text: lambda TripIEx2


text: National Botanical Research Institute

text: live

characteristic: INSDC status

text: Generic

text: cDNA insert library of T. aestivum prepared using pGEM-T Easyvector

text: 30 day old wheat plant

text: leaf

text: Triticum aestivum

text: EcoRI

text: SpeI

text: leaf blade

text: Triticum aestivum leaf cDNA library from 30-day old plant

text: Plasmid

text: pGEM-T Easy


text: Centre for Environmental Stress and Adaptation Research, Bio21 Institute, Genetics Department, University of Melbourne

text: live

text: Generic

characteristic: NCBI submission model

text: Embryonic RNA was extracted using the mRNA Isolation System from Gibco-BRL; A unidirectional cDNA library was constructed in the EcoRI/XhoI sites of the Lambda ZAP II vector (Stratagene), and sequenced using T3 primer (5'-AATTAACCCTCACTAAAGGG). For strain information please refer to Chen et al. Insect Biochemistry and Molecular Biology 28 (1998) 601-612.

text: Embryos 0-7 hr

text: XL-1 Blue

characteristic: lab-host

text: Lucilia cuprina

text: EcoRI

text: XhoI

text: Non Modifier (Seeking)

characteristic: strain

text: Lucilia cuprina embryonic cDNA library

text: plasmid

text: alpha ZAP II


text: live

text: Generic

characteristic: NCBI submission package

text: Total RNA was extracted using RNeasy kit (Qiagen). cDNA was prepared using the Clontech SMART cDNA Library Construction kit, and ligated into pTriplE2 vector. Ligation mix was transformed into BM25.8 Blue cells. Plasmids were sequenced using 5' TriplEx2 sequencing primer (5'-TCCGAGATCTGGACGAGC).

text: first instar larvae

text: Lucilia cuprina

text: Sfi-I A

text: Sfi-I B

text: Lucilia cuprina first instar larval cDNA library

text: plasmid


text: live

text: Generic

characteristic: description

text: third instar larvae

text: Lucilia cuprina

text: Sfi-I A

text: Sfi-I B

text: Lucilia cuprina third instar larval cDNA library

text: plasmid


text: Indian Agricultural Research Institute

text: live

text: Generic

text: HD2329 + Lr28

characteristic: cultivar

text: Differentially regulated transcripts in incompatible interaction of wheat challenged with Puccinia triticina pathotype 77-5 (syn. 121 R63-1)

text: The seven days old seedling stage

characteristic: dev-stage

text: Triticum aestivum

text: Lgh

text: Leaf

text: Transcript derived fragments (TDFs) from resistant seven day old seedling wheat leaves inoculated by leaf rust fungus

text: PCR TRAP


text: Indian Agricultural Research Institute

text: live

text: Generic

text: Differentially regulated transcripts in compatible interaction of wheat challenged with Puccinia triticina pathotype 77-5 (syn. 121 R63-1)

text: The seven days old seedling stage

text: Triticum aestivum

characteristic: organism

text: Lgh

text: Leaf

text: Transcript derived fragments (TDFs) from susceptible seven day old seedling wheat leaves inoculated by leaf rust fungus

characteristic: title

text: PCR TRAP


characteristic: External Id

text: The University of Tokyo

text: live

text: Generic

text: adult

text: Pinctada fucata

text: mantle

text: Pinctada fucata mantle adult


text: Genoscope - Centre National de Sequencage

text: live

text: Generic

text: Lampetra fluviatilis

text: Lampetra fluviatilis library (Genoscope - CEA)


text: Proimi-Conicet

text: live

text: Generic

text: Chile:Laguna Turquesa, Salar de Ascotan

characteristic: country

text: salt flat water

characteristic: isolation-source

text: uncultured bacterium

text: OPJ10-cDNA-LT uncultured bacterium from salt flat water


text: Proimi-Conicet

characteristic: INSDC center name

text: live

text: Generic

text: Chile:Laguna Turquesa, Salar de Ascotan

text: salt flat water

text: uncultured Gluconobacter sp.

text: OPJ10-cDNA-LT uncultured Gluconobacter sp. from salt flat water


text: Proimi-Conicet

characteristic: INSDC first public

text: live

text: Generic

text: Chile:Laguna Turquesa, Salar de Ascotan

text: salt flat water

text: uncultured Cyanobacterium sp.

text: OPJ10-cDNA-LT uncultured Cyanobacterium sp. from salt flat water


text: Proimi-Conicet

text: live

text: Generic

text: Chile:Laguna Turquesa, Salar de Ascotan

text: salt flat water

text: uncultured Metallosphaera sp.

text: OPJ10-cDNA-LT uncultured Metallosphaera sp. from salt flat water


text: Proimi-Conicet

text: live

text: Generic

text: Chile:Laguna Turquesa, Salar de Ascotan

text: salt flat water

text: uncultured Aspergillus

text: OPJ10-cDNA-LT uncultured Aspergillus sp. from salt flat water


text: Proimi-Conicet

text: live

text: Generic

text: Chile:Laguna Turquesa, Salar de Ascotan

text: salt flat water

text: uncultured Streptomyces sp.

text: OPJ10-cDNA-LT uncultured Streptomyces sp.from salt flat water


text: Proimi-Conicet

text: live

text: Generic

text: Chile:Laguna Turquesa, Salar de Ascotan

text: salt flat water

text: uncultured Leptospirillum sp.

text: OPJ10-cDNA-LT uncultured Leptospirillum sp. from salt flat water


text: Proimi-Conicet

text: live

text: Generic

text: Chile:Laguna Turquesa, Salar de Ascotan

text: salt flat water

text: uncultured Bacteroides sp.

text: OPJ10-cDNA-LT uncultured Bacteroides sp. from salt flat water


text: Proimi-Conicet

text: live

text: Generic

text: Chile:Laguna Turquesa, Salar de Ascotan

text: salt flat water

text: uncultured Bacillus sp.

text: OPJ10-cDNA-LT uncultured Bacillus sp. from salt flat water


text: Proimi-Conicet

text: live

text: Generic

text: Chile:Laguna Turquesa, Salar de Ascotan

text: salt flat water

text: uncultured Mycobacterium sp.

text: OPJ10-cDNA-LT uncultured Mycobacterium sp. from salt flat water


text: Proimi-Conicet

text: live

text: Generic

text: Chile:Laguna Turquesa, Salar de Ascotan

text: salt flat water

text: uncultured Salinibacter sp.

text: OPJ10-cDNA-LT uncultured Salinibacter sp. from salt flat water


text: Leibniz Institute for Age Research - Fritz Lipmann Institute

text: live

text: Generic

text: cDNA was prepared as described in: Wodniok S, Simon A, Glockner G, Becker B: Gain and loss of polyadenylation signals during evolution of green algae. BMC Evolutionary Biology 2007, 7:65.

text: Coleochaete scutata

text: Coleochaete scutata cDNA library


text: live

text: Generic

text: Klebsormidium subtile

text: Klebsormidium subtile cDNA library


text: College of Horticulture, Northwest A&F University

text: live

text: Generic

text: Qinguan

text: Growing stage

text: E.coli TOPO10

text: Malus domestica

text: Young leaf

characteristic: tissue-type

text: Young leaves inoculated with Marssonina coronaria SSH cDNA library of Qinguan apple (Malus domestica)

text: pGEM-T Easy Vector

characteristic: vector


text: CSIRO

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: late nymph

text: Apotrechus illawarra

text: both

characteristic: sex

text: labial gland acinus

text: Apotrechus illawarra labial gland acinus library


text: College of Life Sciences, Wuhan University

text: live

text: Generic

characteristic: NCBI submission model

text: petit havana SR1

text: cDNA of the asymmetric two-celled proembryos was subtracted and cloned into vector for screening, then the identified clones were sequenced for EST analysis.

text: two-celled proembryo

text: E.coli

characteristic: lab-host

text: Nicotiana tabacum

text: Hermaphrodite

text: embryo

text: Nicotiana tabacum petit havana SR1 asymmetric two-celled proembryo suppression subtractive cDNA library

text: Plasmid

text: pGEMT Easy


text: live

text: Generic

characteristic: NCBI submission package

text: petit havana SR1

text: cDNA of the symmetric two-celled proembryos was subtracted and cloned into vector for screening, then the identified clones were sequenced for EST analysis.

characteristic: description

text: two-celled proembryo

text: E.coli

text: Nicotiana tabacum

text: Hermaphrodite

text: embryo

text: Nicotiana tabacum petit havana SR1 symmetric two-celled proembryo suppression subtractive cDNA library

text: Plasmid

text: pGEMT Easy


text: live

text: Generic

text: petit havana SR1

characteristic: cultivar

text: cDNA of the zygote was subtracted against asymmetric two-celled embryo and cloned into vector for screening, then the identified clones were sequenced for EST analysis.

text: zygote

characteristic: dev-stage

text: E.coli

text: Nicotiana tabacum

text: Hermaphrodite

text: Nicotiana tabacum petit havana SR1 zygote suppression subtractive cDNA library (against asymmetric two-celled embryo)

text: Plasmid

text: pGEMT Easy


text: live

text: Generic

text: petit havana SR1

text: cDNA of the zygote was subtracted against symmetric two-celled embryo and cloned into vector for screening, then the identified clones were sequenced for EST analysis.

text: zygote

text: E.coli

text: Nicotiana tabacum

characteristic: organism

text: Hermaphrodite

text: Nicotiana tabacum petit havana SR1 zygote suppression subtractive cDNA library (against symmetric two-celled embryo)

characteristic: title

text: Plasmid

text: pGEMT Easy


characteristic: External Id

text: Institute of Life Sciences

text: live

text: Generic

text: Suppressive subtraction hybridization was performed using the SuperSMART cDNA Synthesis Kit and the Clontech PCR-Select cDNA Subtraction Kit taking the mRNA from the programmed cell death leaf and internodes samples as tester and that from normal leaf and internodes as driver. The secondary PCR product was non-directionally cloned in a pGEM-T easy vector (Promega) using PCR Cloning Kit. The EST clones were sequenced using the M13 Forward primer at Chromous Biotech.

text: fully developed normal as well as programmed cell death plants, grown in natural condition

text: Bambusa bambos

text: EcoRI

text: EcoRI

text: leaf and internode

text: Bambusa arundinacea forward library from PCR-based suppressive subtraction hybridization

text: Plasmid

text: pGEM-T easy


text: International Centre for Genetic Engineering and Biotechnology (ICGEB)

text: live

text: Generic

text: first and second instar

text: Orseolia oryzae

characteristic: strain

text: Whole maggot

text: Asian rice gall midge cDNA library

text: pCR 4 TOPO


characteristic: INSDC center name

text: live

text: Generic

text: Bikaneri Narma

text: 0-20 Days post anthesis

text: Boll

characteristic: organ

text: Gossypium hirsutum

text: EcoRI

text: XhoI

text: Ovule and fiber

text: Subtracted cDNA library from Gossypium hirsutum L. variety Bikaneri Narma

text: Lambda Zap


text: University of Bergen

characteristic: INSDC first public

text: live

text: Generic

text: Total RNA was extracted from several individuals using TRIzol (Invitrogen) followed by polyA+ RNA selection using PolyATract (Promega) prior to cDNA library construction using the Creator SMART cDNA Library construction kit (Clontech) by GENterprise (Mainz, Germany) with directional cloning using a modified pSPORT vector. Around 1,000 clones were sequenced using an ABI 3730 (Applied Biosystems).

text: Scutopus ventrolineatus

text: Sfi I A

text: SFi I B

text: Several whole animals

characteristic: tissue-type

text: Scutopus ventrolineatus ESTs

text: plasmid

text: pSport Mod x Sfi I


text: University of Bergen

characteristic: INSDC last update

text: live

text: Generic

text: Total RNA was extracted from several individuals using the RNeasy kit (Qiagen) prior to cDNA library construction using the Creator SMART cDNA Library construction kit (Clontech) by GENterprise (Mainz, Germany) with directional cloning using a modified pSPORT vector. Around 1,000 clones were sequenced using an ABI 3730 (Applied Biosystems).

text: Wirenia argentea

text: Sfi I A

text: SFi I B

text: Several whole animals (starved 2 months)

text: Wirenia argentea ESTs

text: plasmid

text: pSport Mod x Sfi I

characteristic: vector


text: Korea University

text: live

characteristic: INSDC status

text: Generic

text: Liriodendron tulipifera

text: Liriodendron tulipifera complementary DNA sequencing of expressed sequence tags


text: Nanfan Hospital

text: live

text: Generic

characteristic: NCBI submission model

text: ayu SSH library enriched for genes up-regulated by Listonella anguillarum

text: juvenile

text: Plecoglossus altivelis

text: liver

text: Listonella anguillarum infected ayu SSH Forward Library


text: Nanfan Hospital

text: live

text: Generic

text: ayu SSH library enriched for genes down-regulated by Listonella anguillarum

text: juvenile

text: Plecoglossus altivelis

text: liver

text: Listonella anguillarum infected ayu SSH Reverse Library


text: Universidade Federal do Parana

text: live

text: Generic

text: Cateto Zebu

text: Construction of cDNA libraries was performed using Creator SMART cDNA Library Construction Kit (Clontech Takara Bio Company)

text: Oryza sativa Japonica Group

text: root

text: Rice roots inoculated with the diazotroph Herbaspirillum seropedicae strain SmR1

text: Plasmid

text: pDNR-LIB


text: Universidade Federal do Parana

text: live

text: Generic

text: Cateto Zebu

text: Oryza sativa Japonica Group

text: root

text: Rice roots non-inoculated with the diazotroph Herbaspirillum seropedicae strain SmR1

text: Plasmid

text: pDNR-LIB


text: University of Victoria

text: live

text: Generic

text: Yeast-like cells

characteristic: cell type

text: Size fractionated cDNA corresponding to 400-200 bp cDNA library

text: Escherichia coli (DH12S ultracompetent cells)

characteristic: lab-host

text: Ophiostoma novo-ulmi subsp. novo-ulmi

text: onovyeastlmw Ophiostoma novo-ulmi subsp. novo-ulmi cDNA

text: Phagemid

text: pBlueScript II SK


text: Universidad de Salamanca

text: live

text: Generic

characteristic: NCBI submission package

text: Trichoderma lixii

text: CECT 2413

text: Hypocrea lixii CECT 2413 mycelium Lambda Zap Library L92


text: Cardiff University

text: live

text: Generic

text: Alstroemeria peruviana

text: petal

text: Alstroemeria peruviana var. Rebecca COLD petal library

text: Rebecca

characteristic: variety


text: Cardiff University

text: live

text: Generic

text: Alstroemeria peruviana

text: petal

text: Alstroemeria peruviana var. Rebecca DRY petal library

text: Rebecca


text: Cardiff University

text: live

text: Generic

text: Alstroemeria peruviana

text: petal

text: Alstroemeria peruviana var. Rebecca S0S1 petal library

text: Rebecca


text: Cardiff University

text: live

text: Generic

text: Alstroemeria peruviana

text: petal

text: Alstroemeria peruviana var. Rebecca S2S4 petal library

text: Rebecca


text: Cardiff University

text: live

text: Generic

text: Alstroemeria peruviana

text: petal

text: Alstroemeria peruviana var. Rebecca S5S7 petal library

text: Rebecca


text: Cardiff University

text: live

text: Generic

text: Alstroemeria peruviana

text: petal

text: Alstroemeria peruviana var. Rebecca SSH0-2 petal library

text: Rebecca


text: Cardiff University

text: live

text: Generic

text: Alstroemeria peruviana

text: petal

text: Alstroemeria peruviana var. Rebecca SSH2-0 petal library

text: Rebecca


text: Cardiff University

text: live

text: Generic

text: Alstroemeria peruviana

text: petal

text: Alstroemeria peruviana var. Rebecca SSH2-45 petal library

text: Rebecca


text: Cardiff University

text: live

text: Generic

text: Alstroemeria peruviana

text: petal

text: Alstroemeria peruviana var. Rebecca SSH3-2 petal library

text: Rebecca


text: Cardiff University

text: live

text: Generic

text: Alstroemeria peruviana

text: petal

text: Alstroemeria peruviana var. Rebecca SSH45-2 petal library

text: Rebecca


text: University of Washington

text: live

text: Generic

text: all

characteristic: dev-stage

text: Caenorhabditis elegans

text: mixed

characteristic: sex

text: whole worm

text: Caenorhabditis elegans whole worm N2 all


text: Zhongshan School of Medicine, Sun Yat-sen University

text: live

text: Generic

text: The library is constructed with SMART cDNA Library Construction Kit (Clontech Laboratories Inc www.clontech.com)

characteristic: description

text: fourth stage larvae

text: E.coli HST08

text: Angiostrongylus cantonensis

characteristic: organism

text: Sfi IA

text: Sfi IB

text: Angiostrongylus cantonensis fourth stage larvae cDNA library

characteristic: title

text: Phage

text: Lambda TripIEx2


characteristic: External Id

text: United Planters' Association of Southern India-Tea Research Foundation, Tea Research Institute

characteristic: INSDC center name

text: live

text: Generic

characteristic: cultivar

text: Messenger RNA was extracted from healthy and Exobasidium vexans infected tea leaf tissue and subtracted cDNA library was constructed following SSH method(Clonetech)

text: Harvesting stage

text: E.coli JM109

text: Camellia sinensis

text: Leaf

text: Camellia sinensis cultivar SA6 forward SSH library of leaf

text: Plasmid


text: University of Southampton

characteristic: INSDC first public

text: live

text: Generic

text: Human Negroid cervix epitheloid carcinoma

text: HeLa

characteristic: cell-line

text: 5' RACE cDNA library prepared from HeLa cell mRNA using the Ambion First Choice RLM RACE kit

text: Homo sapiens

text: EcoRI

text: EcoRI

text: Female

text: Cervix

characteristic: tissue-type

text: HeLa cell 5' RACE library

text: Plasmid

text: pGEMT easy


text: University of Southampton

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Human Caucasian breast adenocarcinoma

text: 5' RACE cDNA library prepared from MCF7 cell mRNA using the Ambion First Choice RLM RACE kit

text: Homo sapiens

text: EcoRI

text: EcoRI

text: Female

text: Breast

text: MCF7 cell 5' RACE library

text: Plasmid

text: pGEMT easy

characteristic: vector


text: University of Southampton

text: live

text: Generic

characteristic: NCBI submission model

text: Human Embryo Kidney

characteristic: cell type

text: HeLa

text: 5' RACE cDNA library prepared from HEK293 cell mRNA using the Ambion First Choice RLM RACE kit

text: Homo sapiens

text: EcoRI

text: EcoRI

text: Female

text: Kidney, embryo

text: HEK293 cell 5' RACE library

text: Plasmid

text: pGEMT easy


text: Boyce Thompson Institute for Plant Research

text: live

text: Generic

characteristic: NCBI submission package

text: Dulce

text: More information is available at http://www.icugi.org

text: Cucumis melo

text: callus

text: CM-DEa library

text: Plasmid

text: pBluescript


text: live

text: Generic

text: Cucumis melo

text: callus

text: CM-PEa library

text: Plasmid

text: pBluescript


text: live

text: Generic

text: Piel de Sapo T-111

text: Cucumis melo

text: callus

text: CM-TEa library

text: Plasmid

text: pBluescript


text: live

text: Generic

text: Vedrantais

text: Cucumis melo

text: callus

text: CM-VEa library

text: Plasmid

text: pBluescript


text: live

text: Generic

text: Piel de Sapo T-111

text: a mixture of fruits at 10, 20, 30 and 45 DPA

characteristic: dev-stage

text: Cucumis melo

text: fruit

text: SFTP2 library

text: Plasmid

text: pGEM-Teasy


text: live

text: Generic

text: Vedrantais

text: a mixture of fruits at 10, 20, 30 and 32 DPA

text: Cucumis melo

text: fruit

text: VFTP2 library

text: Plasmid

text: pGEM-Teasy


text: live

text: Generic

text: a mixture of fruits at 10, 20, 30 and 42 DPA

text: Cucumis melo

text: fruit

text: PFTP2 library

text: Plasmid

text: pGEM-Teasy


text: live

text: Generic

text: Dulce

text: Cucumis melo

text: fruit

text: DFTP2 library

text: Plasmid

text: pGEM-Teasy


text: live

text: Generic

text: C1 is the most initial stage where the flowers are around 1 mm in the longitudinal axis. C3 is the stage where the future fruit shape is already defined and where first stamens are visible. C5 is the stage just before anthesis (1-2cm). More information is available at http://www.icugi.org

text: a mixture of hermaphrodite flowers at three developmental stages (C1, C3, C5)

text: Cucumis melo

text: hermaphrodite flowers

text: PFLP2 library

text: Plasmid

text: pGEM-Teasy


text: live

text: Generic

text: Dulce

text: Cucumis melo

text: hermaphrodite flowers

text: DFLP2 library

text: Plasmid

text: pGEM-Teasy


text: live

text: Generic

text: Piel de Sapo T-111

text: Cucumis melo

text: hermaphrodite flowers

text: SFLP2 library

text: Plasmid

text: pGEM-Teasy


text: live

text: Generic

text: Vedrantais

text: Cucumis melo

text: hermaphrodite flowers

text: VFLP2 library

text: Plasmid

text: pGEM-Teasy


text: live

text: Generic

text: Piel de Sapo T-111

text: Cucumis melo

text: cotyledon infected with MNSV-Malfa5

text: MNCP2 library

text: Plasmid

text: pGEM-Teasy


text: live

text: Generic

text: Piel de Sapo T-111

text: Cucumis melo

text: root infected with MNSV-Malfa5

text: MNRP2 library

text: Plasmid

text: pGEM-Teasy


text: live

text: Generic

text: Piel de Sapo T-111

text: Cucumis melo

text: leaf infected with MNSV-Malfa5

text: MNFG2 library

text: Plasmid

text: pGEM-Teasy


text: Agriculture and Agri-Food Canada; Potato Research Centre

text: live

text: Generic

text: Shepody

text: Greenhouse plants before flowering fertilized with 7.5 mM NO3 nitrogen. The detail of the plant treatment is described in: Li XQ, Sveshnikov D, Zebarth BJ, Tai H, De Koeyer D, Millard P, Haroon M, Singh M (2010) Detection of nitrogen sufficiency in potato plants using gene expression markers. American Journal of Potato Research, 87:50-59.

text: SOLR

characteristic: lab-host

text: Roots

characteristic: organ

text: Solanum tuberosum

text: EcoRI

text: XhoI

text: SRN (roots from Solanum tuberosum Shepody fertilized with nitrogen)

text: pBluescript SK(-)


text: live

text: Generic

text: Shepody

text: Greenhouse plants before flowering treated with zero nitrogen supply 3 days before sampling. The detail of the plant treatment is described in: Li XQ, Sveshnikov D, Zebarth BJ, Tai H, De Koeyer D, Millard P, Haroon M, Singh M (2010) Detection of nitrogen sufficiency in potato plants using gene expression markers. American Journal of Potato Research, 87:50-59.

characteristic: description

text: SOLR

text: Roots

text: Solanum tuberosum

characteristic: organism

text: EcoRI

text: XhoI

text: SRA (roots from unfertilized Solanum tuberosum Shepody)

text: pBluescript SK(-)


characteristic: External Id

text: live

text: Generic

text: Shepody

text: SOLR

text: Petioles

text: Solanum tuberosum

text: EcoRI

text: XhoI

text: SPN (petioles from Solanum tuberosum Shepody fertilized with nitrogen)

characteristic: title

text: pBluescript SK(-)


characteristic: INSDC center name

text: live

text: Generic

text: Shepody

characteristic: cultivar

text: SOLR

text: Petioles

text: Solanum tuberosum

text: EcoRI

text: XhoI

text: SPA (petioles from unfertilized Solanum tuberosum Shepody)

text: pBluescript SK(-)


characteristic: INSDC first public

text: live

text: Generic

text: Shepody

text: Greenhouse plants before flowering fertilized with 7.5 mM NO3 nitrogen. RNA was extracted from the sixth leaf from the top of the plant. The detail of the plant treatment is described in: Li XQ, Sveshnikov D, Zebarth BJ, Tai H, De Koeyer D, Millard P, Haroon M, Singh M (2010) Detection of nitrogen sufficiency in potato plants using gene expression markers. American Journal of Potato Research, 87:50-59.

text: SOLR

text: Leaves

text: Solanum tuberosum

text: EcoRI

text: XhoI

text: SLN (leaves from Solanum tuberosum Shepody fertilized with nitrogen)

text: pBluescript SK(-)


text: live

text: Generic

text: Shepody

text: Greenhouse plants before flowering treated with zero nitrogen supply 3 days before sampling. RNA was extracted from the sixth leaf from the top of the plant. The detail of the plant treatment is described in: Li XQ, Sveshnikov D, Zebarth BJ, Tai H, De Koeyer D, Millard P, Haroon M, Singh M (2010) Detection of nitrogen sufficiency in potato plants using gene expression markers. American Journal of Potato Research, 87:50-59.

text: SOLR

text: Leaves

text: Solanum tuberosum

text: EcoRI

text: XhoI

text: SLA (leaves from unfertilized Solanum tuberosum Shepody)

text: pBluescript SK(-)


text: JAIST, CNMT

text: live

text: Generic

characteristic: cell-line

text: Mus musculus

text: Mus musculus P19


text: MPI for Molecular Genetics

characteristic: INSDC last update

text: live

text: Generic

text: T.H. Struck

characteristic: collected-by

characteristic: collection-date

text: Germany:Sylt, List

characteristic: country

text: adult

text: 55.03 N 8.42 E

characteristic: lat-lon

text: Arenicola marina

text: chloragog and foregut

characteristic: tissue-type

text: Arenicola marina chloragog and foregut adult


text: MPI for Molecular Genetics

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: C. Bleidorn

text: France:Roscoff

text: adult

characteristic: dev-stage

text: 48.73 N 3.97 W

text: Eulalia clavigera

text: whole animal

text: Eulalia clavigera whole animal adult


text: MPI for Molecular Genetics

text: live

text: Generic

text: T.H. Struck

text: Germany:Osnabrueck

text: adult

text: sea water aquarium

characteristic: isolation-source

text: 52.04 N 8.53 E

text: Eurythoe complanata

characteristic: organism

text: whole animal

text: Eurythoe complanata whole animal adult


characteristic: External Id

text: MPI for Molecular Genetics

text: live

text: Generic

text: T.H. Struck

text: Germany:Sylt, List

text: adult

text: 55.03 N 8.42 E

text: Lanice conchilega

text: whole animal

text: Lanice conchilega whole animal adult

characteristic: title


text: MPI for Molecular Genetics

characteristic: INSDC center name

text: live

text: Generic

text: C. Bleidorn

text: Canada:Bamfield

text: adult

text: 48.83 N 125.2 W

text: Lumbrineris zonata

text: whole animal

text: Lumbrineris zonata whole animal adult


text: MPI for Molecular Genetics

characteristic: INSDC first public

text: live

text: Generic

text: T.H. Struck

text: Germany:Helgoland

text: adult

text: 54.17 N 7.89 E

text: Malacoceros fuliginosus

text: whole animal

text: Malacoceros fuliginosus whole animal adult


text: MPI for Molecular Genetics

text: live

text: Generic

text: T.H. Struck

text: Germany:Helgoland

text: adult

text: 54.17 N 7.89 E

text: Pectinaria koreni

text: whole animal

text: Pectinaria koreni whole animal adult


text: MPI for Molecular Genetics

text: live

text: Generic

text: T.H. Struck

text: Germany:Sylt, List

text: adult

text: 55.03 N 8.42 E

text: Scoloplos armiger

text: whole animal

text: Scoloplos armiger whole animal adult


text: MPI for Molecular Genetics

text: live

text: Generic

text: D. Kieselbach

text: France:Roscoff

text: adult

text: 48.73 N 3.97 W

text: Sipunculus nudus

text: whole animal

text: Sipunculus nudus whole animal adult


text: MPI for Molecular Genetics

text: live

text: Generic

text: C. Bleidorn

text: France:Roscoff

text: adult

text: 48.73 N 3.97 W

text: Sthenelais boa

text: whole animal

text: Sthenelais boa whole animal adult


text: MPI for Molecular Genetics

text: live

text: Generic

text: C. Bleidorn

text: Canada:Bamfield

text: adult

text: 48.83 N 125.2 W

text: Syllis pigmentata

text: whole animal

text: Typosyllis pigmentata whole animal adult


text: MPI for Molecular Genetics

text: live

text: Generic

text: T.H. Struck

text: Germany:Osnabrueck

text: adult

text: sea water aquarium

text: 52.04 N 8.53 E

text: Cirratulus sp. AB-2011

text: whole animal

text: Cirratulus sp. AB-2011 whole animal adult


text: MPI for Molecular Genetics

text: live

text: Generic

text: T.H. Struck

text: Germany:Sylt, List

text: adult

text: 55.03 N 8.42 E

text: Ophelia limacina

text: whole animal

text: Ophelia limacina whole animal adult


text: MPI for Molecular Genetics

text: live

text: Generic

text: T.H. Struck

text: Germany:Helgoland

text: adult

text: 54.17 N 7.89 E

text: Flabelligera affinis

text: whole animal

text: Flabelligera affinis whole animal adult


text: MPI for Molecular Genetics

text: live

text: Generic

text: C. Bleidorn

text: Canada:Bamfield

text: adult

text: 48.83 N 125.2 W

text: Onuphis iridescens

text: whole animal

text: Onuphis iridescens whole animal adult