text: University of Bonn

text: live

text: Generic

text: 4 cell stage embryo

text: Bos taurus

characteristic: organism

text: bovine single 4 cell stage embryo cDNA library


status: PUBLIC

characteristic: External Id

text: University of Bonn

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: cDNA library was contructed with the Clontech cDNA library constrution kit

characteristic: description

text: 8 cell stage embryo

tag: attribute

characteristic: dev-stage

text: Bos taurus

text: bovine single 8 cell stage embryo cDNA library

characteristic: title


text: University of Bonn

text: live

text: Generic

text: Blastocyst

text: Bos taurus

text: Bovine single blastocyst stage embryo cDNA library


text: University of Bonn

text: live

text: Generic

text: 2 cell stage embryo

text: Bos taurus

text: bovine 2 cell stage embryo cDNA library


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.7 kb. Constructed by Life Technologies. Note: This is a Xenopus Gene Collection (XGC) library.

text: DH10B (phage-resistant)

characteristic: lab-host

text: Xenopus laevis

text: NotI

text: SalI

text: embryo (stages 24-25)

characteristic: tissue-type

text: phagemid (ampicillin resistant)

characteristic: vector


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.4 kb. Constructed by Life Technologies. Note: This is a Xenopus Gene Collection (XGC) library.

text: DH10B (phage-resistant)

text: liver

characteristic: organ

text: Xenopus laevis

text: NotI

text: SalI

text: phagemid (ampicillin resistant)


text: Institute of Basic Medical Science, Peking Union Medical College

text: live

text: Generic

text: Homo sapiens

text: bone marrow

text: human B lymphocyte cDNA library


text: Shirakawa Institute of Animal Genetics

text: live

text: Generic

text: Poly A was deleted from a Not1 site

text: fetus

text: Bos taurus

text: kidney

text: Bos taurus kidney fetus


text: Shirakawa Institute of Animal Genetics

text: live

text: Generic

text: fetus

text: Bos taurus

text: liver

text: Bos taurus liver fetus


text: Shirakawa Institute of Animal Genetics

text: live

text: Generic

text: Poly A was deleted from a Not1 site

text: fetus

text: Bos taurus

text: lung

text: Bos taurus lung fetus


text: Shirakawa Institute of Animal Genetics

text: live

text: Generic

text: an adipocyte cell line

characteristic: cell type

text: Poly A was deleted from a Not1 site

text: Bos taurus

text: Bos taurus adipocyte cell line


text: Shirakawa Institute of Animal Genetics

text: live

text: Generic

text: Poly A was deleted from a Not1 site

text: fetus

text: Bos taurus

text: ovary

text: Bos taurus ovary fetus


text: Shirakawa Institute of Animal Genetics

text: live

text: Generic

text: fetus

text: Bos taurus

text: rumen

text: Bos taurus rumen fetus


text: PUMC

text: live

text: Generic

text: Rattus norvegicus

text: Wistar

characteristic: strain

text: seminiferous epithelium

text: Rattus norvegicus seminiferous epithelium DDRT-PCR


text: University of Texas Medical Branch

text: live

text: Generic

text: Homo sapiens

characteristic: organism

text: colon cancer

text: Homo sapiens colon cancer


characteristic: External Id

text: Hunan Medical University

text: live

text: Generic

text: spermatogenesis cells

text: adult

text: Mus musculus

text: male

characteristic: sex

text: testis

text: mouse testis apoptosis subtraction cDNA library

text: pUCm-T


characteristic: INSDC first public

text: live

text: Generic

text: cDNA made by oligo-dT priming. Directionally cloned into EcoRI/XhoI sites using the following 5' adaptor: GGCACGAG(G). Library constructed by Ling Hong in the laboratory of Gerald M. Rubin (University of California, Berkeley) using ZAP-cDNA synthesis kit (Stratagene) and Superscript II RT (Life Technologies).

text: DH10B (phage-resistant)

text: pancreas

text: Homo sapiens

text: XhoI

text: EcoRI

text: adenocarcinoma

text: plasmid (chloramphenicol resistant, grown at 30C)


text: US Department of Agriculture, Agriculture Research Service, Pacific West Area, Western Regional Research Center

characteristic: INSDC center name

characteristic: INSDC last update

text: live

text: Generic

text: Brevor (soft, white, winter, common wheat)

characteristic: cultivar

text: Embryos were cut from mature, dormant seeds and imbibed in 25 microM ABA (abscisic acid) in 5 mM Mes buffer, pH 5.7, for 12 hr at 22 C. The tissue, total RNA, and poly(A) RNA were prepared by Steven Verhey in M.K. Walker-Simmons's lab (USDA-ARS, Washington State Univ., Pullman, Washington 99164-6420. A cDNA library was made by Clontech using a combination of random and oligo dT primers. Library was plated and archived by Russell Johnson (Colby College, ME/Walker-Simmons' lab). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

text: Mature dormant seeds

text: E. coli DH12S

text: Triticum aestivum

text: EcoRI

text: XhoI

text: Seed embryo

text: Wheat ABA-treated embryo cDNA library

text: Plasmid


text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Chinese Spring

text: Seeds were germinated and grown at 4 C for 5 weeks. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

text: Five-week old seedling

text: E. coli SOLR

text: Triticum aestivum

text: EcoRI

text: XhoI

text: Crown tissue of seedling

text: Wheat vernalized crown cDNA library

text: Phagemid

text: Lambda Uni-ZAP XR, excised phagemid


text: National University of Ireland Galway (NUIG)

text: live

text: Generic

text: An Atlantic salmon spleen cDNA library was constructed using the Lambda Zap Express/Gigapack cloning kit (Stratagene cloning systems). cDNA synthesis was carried out using an oligo-(dT) primer for the reverse transcription of 5ug of mRNA and the library was constructed by directional cloning EcoR1-Xho1 based on manufacturers instructions. An insert:vector ligation ratio of 1:5 was chosen as most optimum . The lambda library was packaged with Gigapack III gold packaging extracts and plated on the E. coli cell line XL1-Blue MRF'

characteristic: description

text: adult

characteristic: dev-stage

text: spleen

text: Salmo salar

text: spleen

text: Atlantic salmon Lambda Zap Express spleen cDNA library

characteristic: title

text: Phage

text: Lambda ZAP Express


text: live

text: Generic

text: An Atlantic salmon testis cDNA library was constructed using the Lambda Zap Express/Gigapack cloning kit (Stratagene cloning systems). cDNA synthesis was carried out using an oligo-(dT) primer for the reverse transcription of 5ug of mRNA and the library was constructed by directional cloning EcoR1-Xho1 based on manufacturers instructions. An insert:vector ligation ratio of 1:5 was chosen as most optimum . The lambda library was packaged with Gigapack III gold packaging extracts and plated on the E. coli cell line XL1-Blue MRF'.

text: adult

text: testis

text: Salmo salar

text: male

text: testis

characteristic: tissue-type

text: Atlantic salmon Lambda Zap Express testis cDNA library

text: Phage

text: Lambda ZAP Express

characteristic: vector


text: live

text: Generic

text: An Atlantic salmon ovary cDNA library was constructed using the Lambda Zap Express/Gigapack cloning kit (Stratagene cloning systems). cDNA synthesis was carried out using an oligo-(dT) primer for the reverse transcription of 5ug of mRNA and the library was constructed by directional cloning EcoR1-Xho1 based on manufacturers instructions. An insert:vector ligation ratio of 1:5 was chosen as most optimum . The lambda library was packaged with Gigapack III gold packaging extracts and plated on the E. coli cell line XL1-Blue MRF'.

text: adult

text: ovary

characteristic: organ

text: Salmo salar

text: female

text: ovary

text: Atlantic salmon Lambda Zap Express ovary cDNA library

text: Phage

text: Lambda ZAP Express


text: live

text: Generic

text: An Atlantic salmon muscle cDNA library was constructed using the Lambda Zap Express/Gigapack cloning kit (Stratagene cloning systems). cDNA synthesis was carried out using an oligo-(dT) primer for the reverse transcription of 5ug of mRNA and the library was constructed by directional cloning EcoR1-Xho1 based on manufacturers instructions. An insert:vector ligation ratio of 1:5 was chosen as most optimum . The lambda library was packaged with Gigapack III gold packaging extracts and plated on the E. coli cell line XL1-Blue MRF'.

text: adult

text: muscle

text: Salmo salar

text: muscle

text: Atlantic salmon Lambda Zap Express muscle cDNA library

text: Phage

text: Lambda ZAP Express


text: live

text: Generic

text: An Atlantic salmon liver cDNA library was constructed using the Lambda Zap Express/Gigapack cloning kit (Stratagene cloning systems). cDNA synthesis was carried out using an oligo-(dT) primer for the reverse transcription of 5ug of mRNA and the library was constructed by directional cloning EcoR1-Xho1 based on manufacturers instructions. An insert:vector ligation ratio of 1:5 was chosen as most optimum . The lambda library was packaged with Gigapack III gold packaging extracts and plated on the E. coli cell line XL1-Blue MRF'.

text: adult

text: liver

text: Salmo salar

text: liver

text: Atlantic salmon Lambda Zap Express liver cDNA library

text: Phage

text: Lambda ZAP Express


text: live

text: Generic

text: An Atlantic salmon brain cDNA library was constructed using the Lambda Zap Express/Gigapack cloning kit (Stratagene cloning systems). cDNA synthesis was carried out using an oligo-(dT) primer for the reverse transcription of 5ug of mRNA and the library was constructed by directional cloning EcoR1-Xho1 based on manufacturers instructions. An insert:vector ligation ratio of 1:5 was chosen as most optimum . The lambda library was packaged with Gigapack III gold packaging extracts and plated on the E. coli cell line XL1-Blue MRF'.

text: adult

text: brain

text: Salmo salar

text: brain

text: Atlantic salmon Lambda Zap Express brain cDNA library

text: Phage

text: Lambda ZAP Express


text: Centro de Pesquisas Rene Rachou - FIOCRUZ

text: live

text: Generic

text: Blunt-ended cloning (SureClone Kit). CaCl2 transformation of competent XL1-blue cells

text: Egg

text: Schistosoma mansoni

text: Mix

text: LE

characteristic: strain

text: Egg cDNA expression randomly amplified mini-library in pUC18


text: live

text: Generic

text: Egg

text: Schistosoma mansoni

characteristic: organism

text: Mix

text: LE

text: Lung stage worms cDNA expression randomly amplified mini-library in pUC18


characteristic: External Id

text: live

text: Generic

text: Egg

text: Schistosoma mansoni

text: Mix

text: LE

text: Adult worm cDNA expression randomly amplified mini-library in pUC18


text: Instituto de Investigaciones Bioquimicas Fundacion Campomar

text: live

text: Generic

text: epithelial

characteristic: cell type

text: T84 (ATCC number CCL-248)

characteristic: cell-line

text: cDNA made from total RNA isolated from T84 cells

text: Homo sapiens

text: male

characteristic: sex

text: colorectal carcinoma; colon; from metastatic site: lung

text: cDNA from T84 cells


text: Michigan State University

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: 5-13 days after flowering

text: E.coli

characteristic: lab-host

text: Developing seed

text: Arabidopsis thaliana

text: EcoRI

text: XhoII

text: Columbia

text: seed

text: Arabidopsis developing seed

text: Plasmid

text: pBluescript SK-


text: Shanghai Institute of Physiology, Chinese Academy of Sciences

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: It was prepared with RAP-PCR technology.

text: adult

text: Rattus norvegicus

text: female

text: Sprague-Dawley

text: brain

text: Rat cDNA library generated with RAP-PCR technology

text: Plasmid

text: T/A Easy Vector


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: Double-stranded cDNA was prepared from cell line RNA. 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.75 kb (range 0.9-4.0 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA).

characteristic: description

text: DH10B (T1 phage-resistant)

text: bone marrow

text: Homo sapiens

text: SfiI (ggccgcctcggcc)

text: SfiI (ggccattatggcc)

text: from chronic myelogenous leukemia

characteristic: title

text: plasmid (chloramphenicol resistant, grown at 30C)

text: pDNR-LIB (Clontech)


text: Washington University School of Medicine

text: live

text: Generic

text: Williams 82

characteristic: cultivar

text: This cDNA library was constructed from mRNA isolated from etiolated hypocotyl tissue of 9-10 day old seedlings of the cultivar Williams 82. Complementary DNA was synthesized from mRNA using a primer consisting of a poly(dT) primer with a XhoI restriction site. EcoRI adapters were ligated to the blunt-ended cDNA fragments followed by digestion with EcoRI and XhoI. The cDNA fragments were directionally cloned into the EcoRI-XhoI restriction site of the pBluescript vector. The ligated cDNA fragments were transformed into DH10B host cells (Gibco BRL). This library was constructed by Dr. Randy Shoemaker.

text: Glycine max

text: EcoRI

text: XhoI

text: Hypocotyl, 9-10 day old etiolated seedlings

characteristic: tissue-type

text: plasmid

text: pBluescriptII SK+

characteristic: vector


text: live

text: Generic

text: Williams

text: This cDNA library was constructed from mRNA isolated from seeds germinated for three days of the cultivar Williams. Complementary DNA was synthesized from mRNA using a primer consisting of a poly(dT) sequence with a NotI restriction site. EcoRI adapters were ligated to the blunt-ended cDNA fragments followed by digestions with EcoRI and NotI. The cDNA fragments were directionally cloned into the EcoRI-NotI restriction site of the pT7T3-Pac vector. The ligated cDNA fragments were transformed into DH10B host cells (Gibco BRL). This library was constructed by Dr. Randy Shoemaker.

text: Glycine max

characteristic: organism

text: EcoRI

text: NotI

text: Germinating Seeds

text: plasmid

text: pT7T3Pac (Pharmacia)


characteristic: External Id

text: IBVM, INRA-CR Bordeaux

text: live

text: Generic

text: adult

characteristic: dev-stage

text: Escherichia coli JM109

text: flower

characteristic: organ

text: Nicotiana tabacum

text: hermaphrodite

characteristic: strain

text: pGNt

text: Plasmid

text: pGEM-T


text: Institute of Radiation Medicine, Beijing

characteristic: INSDC center name

text: live

text: Generic

text: stomach

text: Homo sapiens

text: EcoRI

text: EcoRI

text: gastrocarcinoma tissue cDNA library

text: plasmid

text: pT AdV


characteristic: INSDC first public

text: live

text: Generic

text: The library was constructed by Dan Howe, University of Kentucky. cDNAs were synthesized from poly(A)+ RNA by oligo d(T) priming and directionally cloned into the Uni-ZAP XR lambda vector. The library was mass excised as phagemids and rescued in SOLR cells. The plasmid library was recovered from the SOLR cells and transformed in mass into DH10B cells for sequencing. WARNING: the library contains a small percentage of cDNAs derived from the bovine host cells.

text: merozoite

text: Sarcocystis neurona

text: EcoRI

text: XhoI

text: cSn 1 S neurona invitro merozoite cDNA

text: phagemid

text: pBluescript SK-


text: University of California at Davis

characteristic: INSDC last update

text: live

text: Generic

text: PCR-based suppression subtractive hybridization cDNA library

text: 3-4 weeks growth

text: E. coli

text: Triphysaria versicolor

text: root-tips ~5mm length

text: Triphysaria versicolor root-tip, early DMBQ-induced transcript cDNA library

text: plasmid

text: pCR2.1 TA Cloning System, Invitrogen, Carlsbad, CA


text: Keele University

text: live

text: Generic

text: isolated from cDNA library through suppression of Fas-mediated apoptosis

text: Homo sapiens

text: bone marrow

text: Homo sapiens bone marrow


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.55 kb. Constructed by Life Technologies. Note: This is a Xenopus Gene Collection (XGC) library.

text: DH10B (phage-resistant)

characteristic: lab-host

text: Xenopus laevis

text: NotI

text: SalI

text: embryo (stage 10)

text: phagemid (ampicillin resistant)


text: Texas A&M University

text: live

characteristic: INSDC status

text: Generic

text: Columbia

text: mRNA derived from early developing seeds (heart stage embryo). cDNA library constructed using the SMART cDNA library technology according to the instructions of the manufacturer (CLONTECH).

text: Heart stage

text: immature seeds

text: Arabidopsis thaliana

text: SacII

text: SpeI

text: heart stage embryo, endosperm and tegument.

text: Arabidopsis thaliana early developing seeds Library

text: Plasmid

text: pGem-T


text: live

text: Generic

characteristic: NCBI submission model

text: SL1-Oligo(dT) PCR-based library. Heterodera glycines cDNA PCR products of size approximately 600-1200 nucleotides containing SL1 on the 5' end and oligo(dT) on the 3' end were non-directionally cloned into pCRII-T PO (Invitrogen) following the Topo TA cloning protocol. The cDNA insert can be excised by digestion with EcoRI. The library was constructed by Dr. Andrew Kloek at Divergence LLC and Brandi Chiapelli and Dr. James McCarter at Washington University, St. Louis. Nematodes are the OP25 strain. Frozen J2 nematodes were provided by Dr. Rick Davis of North Carolina State University

text: enriched for 2nd stage juveniles

text: Heterodera glycines

text: Heterodera glycines J2 SL1 Topo1 Kloek Chiapelli McCarter

text: Ampicillin and Kanamycin resistant

text: pCRII-TOPO


text: live

text: Generic

characteristic: NCBI submission package

text: SL1-Oligo(dT) PCR-based library. Heterodera glycines cDNA PCR products of size >400 nucleotides containing SL1 on the 5' end and oligo(dT) on the 3' end were non-directionally cloned into pCRII-TOPO (Invitrogen) following the Topo TA cloning protocol. The cDNA insert can be excised by digestion with EcoRI. The library was constructed by Dr. Andrew Kloek at Divergence LLC and Brandi Chiapelli and Dr. James McCarter at Washington University, St. Louis. Nematodes are the OP25 strain. Frozen J2 nematodes were provided by Dr. Rick Davis of North Carolina State University

text: enriched for 2nd stage juveniles

text: Heterodera glycines

text: Heterodera glycines J2 SL1 Topo2 Kloek Chiapelli McCarter

text: Ampicillin and Kanamycin resistant

text: pCRII-TOPO


text: Washington University School of Medicine

text: live

text: Generic

text: SL1-Oligo(dT) PCR-based library. Meloidogyne javanica cDNA PCR products of size approximately 600-1200 nucleotides containing SL1 on the 5' end and oligo(dT) on the 3' end were non-directionally cloned into pCRII-TOPO (Invitrogen) following the Topo TA cloning protocol. cDNA products were size separated. The cDNA insert can be excised by digestion with EcoRI. The library was constructed by Dr. Andrew Kloek at Divergence LLC and Brandi Chiapelli and Dr. James McCarter at Washington University, St. Louis. Nematodes were provided by Dr. David Bird of North Carolina State University.

characteristic: description

text: enriched for eggs

text: Meloidogyne javanica

text: Meloidogyne javanica Egg SL1 Topo1 Kloek Chiapelli McCarter

text: Ampicillin and Kanamycin resistant

text: pCRII-TOPO


text: live

text: Generic

text: SL1-Oligo(dT) PCR-based library. Meloidogyne javanica cDNA PCR products of size >400 nucleotides containing SL1 on the 5' end and oligo(dT) on the 3' end were non-directionally cloned into pCRII-TOPO (Invitrogen) following the Topo TA cloning protocol. The cDNA insert can be excised by digestion with EcoRI. The library was constructed by Dr. Andrew Kloek at Divergence LLC and Brandi Chiapelli and Dr. James McCarter at Washington University, St. Louis. Nematodes were provided by Dr. David Bird of North Carolina State University.

text: enriched for eggs

text: Meloidogyne javanica

text: Meloidogyne javanica Egg SL1 Topo2 Kloek Chiapelli McCarter

characteristic: title

text: Ampicillin and Kanamycin resistant

text: pCRII-TOPO


text: Agriculture and Agri-Food Canada

text: live

text: Generic

text: This cDNA library was constructed from polyA+ enriched mRNA from mycelium grown in liquid medium for 3 weeks at 25 C in the dark in a liquid synthetic medium of 2.4 g sucrose, 0.27 g asparagine, 10 mg cholesterol, 10 mg ascorbate, and 2 mg thiamine, per liter, plus salts and minerals. Complementary DNA was synthesized from mRNA using an XhoI-poly(dT) linker-primer. EcoRI adapters were ligated to the blunt-ended cDNA fragments and the products were digested with XhoI for directional cloning into lambda ZAP Express vector. This lambda library was amplified once using E. coli host strain XL1 Blue MRF'. Inserts were then subcloned by mass excision using ExAssist helper phage for conversion into phagemid vector pBK-CMV in E. coli host strain XLOLR. Sequenced using T3 primer: 5' ATT AAC CCT CAC TAA AGG GA 3'.

text: mycelium

text: E. coli strain XLOLR

text: Phytophthora sojae

text: EcoRI

text: XhoI

text: race 2, strain P6497

text: PsojaeMY

text: Plasmid

text: pBK-CMV


text: Agriculture and Agri-Food Canada

text: live

text: Generic

text: Glycine max cultivar Harosoy

characteristic: cultivar

text: This cDNA library was constructed from polyA+ enriched mRNA from etiolated hypocotyls 48 h post-inoculation with Phytophthora sojae zoospores. Complementary DNA was synthesized from mRNA using an XhoI-poly(dT) linker-primer. EcoRI adapters were ligated to the blunt-ended cDNA fragments and the products were digested with XhoI for directional cloning into lambda ZAP Express vector. This lambda library was amplified once using E. coli host strain XL1 Blue MRF'. Inserts were then subcloned by mass excision using ExAssist helper phage for conversion into phagemid vector pBK-CMV in E. coli host strain XLOLR. Sequenced using T3 primer: 5' ATT AAC CCT CAC TAA AGG GA 3'.

text: 8 d old etiolated hypocotyls 48 h post-inoculation with Phytophthora sojae zoospores

text: E. coli strain XLOLR

text: Glycine max/Phytophthora sojae mixed EST library

text: EcoRI

text: XhoI

text: Phytophthora sojae race 2 strain P6497

text: Plant hypocotyls infected with Phytophthora sojae

text: PsojaeHA

text: Plasmid

text: pBK-CMV


text: Agriculture and Agri-Food Canada

text: live

text: Generic

text: This cDNA library was constructed from polyA+ enriched mRNA from zoospores grown in liquid medium. Zoospores were released into water and collected by centrifugation at 2,000g; zoospore-bearing sporangia were induced to develop on 5 to 7 d old mycelium colonies grown on V8 agar by repeated flooding with water. Complementary DNA was synthesized from mRNA using an XhoI-poly(dT) linker-primer. EcoRI adapters were ligated to the blunt-ended cDNA fragments and the products were digested with XhoI for directional cloning into lambda ZAP Express vector. This lambda library was amplified once using E. coli host strain XL1 Blue MRF'. Inserts were then subcloned by mass excision using ExAssist helper phage for conversion into phagemid vector pBK-CMV in E. coli host strain XLOLR. Sequenced using T3 primer: 5' ATT AAC CCT CAC TAA AGG GA 3'.

text: zoospores

text: E. coli strain XLOLR

text: Phytophthora sojae

text: EcoRI

text: XhoI

text: race 2, strain P6497

text: PsojaeZO

text: Plasmid

text: pBK-CMV


text: US Department of Agriculture, Agriculture Research Service, Plant Science and Entomology Unit

text: live

text: Generic

text: Plants were grown in the greenhouse. Spikes were sprayed with Fusarium graminearum (at what stage). Total RNA, and poly(A) RNA were prepared from infected spikes. cDNA was prepared using the SmartTM PCR cDNA synthesis kit from Clonetech. cDNA was cloned into the pGEM-T easy vector from Promega.

text: Adult plant

text: E. coli JM109

text: Triticum aestivum

text: SacII

text: SpeI

text: Spike

characteristic: tissue-type

text: KSU wheat Fusarium graminearum infected spike cDNA library

text: plasmid

text: pGEM-T easy


text: US Department of Agriculture, Agriculture Research Service, Pacific West Area, Western Regional Research Center

text: live

text: Generic

text: Blanco

text: Seeds were germinated aseptically on filter paper and transferred to a hydroponic growth system in a growth chamber when the primary root was 1 cm in length. After a 2-day establishment period, seedlings were subjected to a 5 ppm aluminum stress prior to tissue harvest. Plants were grown in an environmental chamber. The tissue, total RNA, and poly(A) RNA were prepared, and a cDNA library was made (Butler and Gustafson) at University of Missouri, Columbia. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

text: Seedling

text: E. coli DH12S

text: Secale cereale

text: SalI

text: NotI

text: Root tip

text: Secale cereale aluminum-stressed root tip cDNA library

text: Phagemid

characteristic: vector


text: live

text: Generic

text: Blanco

text: Seeds were germinated aseptically on filter paper and transferred to a hydroponic growth system in a growth chamber when the primary root was 1 cm in length. Plants were grown in an environmental chamber until harvesting. The tissue, total RNA, and poly(A) RNA were prepared and a cDNA library was made (Butler and Gustafson) at University of Missouri, Columbia. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

text: Seedlings

text: E. coli DH12S

text: Secale cereale

characteristic: organism

text: SalI

text: NotI

text: Root tip

text: Secale cereale root tip cDNA library

text: Phagemid


characteristic: External Id

text: Monsanto

text: live

text: Generic

text: Bos taurus


text: live

text: Generic

text: Chinese Spring

text: Plants were grown in the greenhouse. Spikes at 20 DAP and seeds at 30 to 45 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

text: Adult plant

characteristic: dev-stage

text: E. coli SOLR

text: Triticum aestivum

text: EcoRI

text: XhoI

text: Spike and seed

text: Wheat 20-45 DAP spike cDNA library

text: Phagemid

text: Lambda Uni-ZAP XR, excised phagemid


text: live

text: Generic

text: Chinese Spring

text: Plants at full-tillering stage were heat stressed under four conditions: (1) 38 C for 2 hours, (2) 38 C for 4 hours, (3) 2 days of cyclic treatment of 38 C for 2 hours, (4) 2 days of cyclic treatment of 38 C for 4 hours, at Texas Tech University (D Zhang in Nguyen lab). Total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton, Malatrasi) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

text: Full tillering stage

text: E. coli SOLR

text: Triticum aestivum

text: EcoRI

text: XhoI

text: Flag leaf

text: Wheat heat stressed flag leaf cDNA library

text: Phagemid

text: Lambda Uni-ZAP XR, excised phagemid


text: University of California, Los Angeles, School of Medicine

text: live

text: Generic

text: Identified using Representational Difference Analysis subtraction. Sequenced using M13 forward and reverse primers.

text: 17 weeks post-fertilization

text: Homo sapiens

text: Brain

text: Homo sapiens fetal cerebral cortex mRNA

text: pBluescript II (-)


text: New Mexico State University

text: live

text: Generic

text: Spores were germinated in the presence of 1% CO2. At 10 days, tissue was ground in liquid nitrogen with sand and mRNA isolated with a modified hot phenol/SDS method. The SMART-PCR method (Clontech) was used for cDNA synthesis from 1 ug of total RNA followed by directional cloning in SfiI digested Triplex2 vector. Plasmid subclones in pTriplEx were recovered by cre-lox excision in E. coli strain BM25.8 and sequenced from the 5' end with the 5'Triplex sequencing primer (Tpx) and the 3' end with the T7 primer (T7). (sfi1=__bp in Comment line of EST file is estimated insert size)

text: 10 days after germination

text: E. coli BM 25.8

text: Rhizophagus intraradices

text: SfiIA

text: SfiIB

text: germinating spore tissue

text: GS Lambda-Triplex, 10 day germinating spore library

text: phagemid

text: Lambda Triplex2


text: live

text: Generic

text: TAM W101

text: Plants were given a gradual stress down to 65% and 78% RWC at Texas Tech University (D. Zhang in HT Nguyen lab). Total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close), at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

text: Full tillering stage

text: E. coli SOLR

text: Triticum aestivum

text: EcoRI

text: XhoI

text: Leaf

text: Wheat drought stressed leaf cDNA library

text: Phagemid

text: Lambda Uni-ZAP XR, excised phagemid


text: live

text: Generic

text: Chinese Spring

text: Spikes at 5, 10, 15 and 20 days after anthesis were heat stressed under two conditions at Texas Tech University (D. Zhang in HT Nguyrn lab): (1) at 38 C for 4 hours and (2) 5 days of cyclic treatment of 38 C for 4 hours. Total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton, Malatrasi) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

text: E. coli SOLR

text: Triticum aestivum

text: EcoRI

text: XhoI

text: Whole spike

text: Wheat heat stressed spike cDNA library

text: Phagemid

text: Lambda Uni-ZAP XR, excised phagemid


text: Advanced Center for Genome Technology, University of Oklahoma

text: live

text: Generic

text: 5' end of cDNA cloned into EcoRI site of pBluescript; 3' end of cDNA cloned into XhoI site of pBluescript

text: Fusarium sporotrichioides

text: EcoRI

text: XhoI

text: Tri 10

text: Fusarium sporotrichioides Tri 10 overexpressed cDNA library

text: phagemid

text: pBlueScript SK-


text: Texas A&M University

text: live

text: Generic

text: vascular smooth muscle

characteristic: cell type

text: cDNA was identified and isolated from Mus musculus benzo(a)pyrene-activated total RNA pool by Differential Display Polymerase Chain Reaction using anchor primer AP3 and arbitrary primer ARP12 from Hieroglyph mRNA Profile Kit 3 (Genomyx Corporation, Foster City, CA). cDNA was subsequently cloned into pCR-TRAP vector and sequenced with Lgh and Rgh primers from both ends using PCR-TRAP Cloning System (GenHunter Corporation, Brookline, MA).

text: Mus musculus

text: female

characteristic: sex

text: aorta

text: 3uM, 8h BaP-treated total RNA


text: Chinese National Human Genome Center at Shanghai

characteristic: INSDC center name

text: live

text: Generic

text: Adult

text: SOLR

text: Homo sapiens

text: EcoRI

text: XhoI

text: corresponding non cancerous liver tissue

text: GLA

text: pBluescript sk(-)


characteristic: INSDC first public

text: live

text: Generic

text: Adult

text: SOLR

text: Homo sapiens

text: EcoRI

text: XhoI

text: GLC

text: pBluescript sk(-)


characteristic: INSDC last update

text: live

text: Generic

text: Adult

text: SOLR

text: Homo sapiens

text: EcoRI

text: XhoI

text: GLD

text: pBluescript sk(-)


text: US Department of Agriculture, Agriculture Research Service, Pacific West Area, Western Regional Research Center

text: live

text: Generic

text: Chinese Spring

text: Plants were grown in the greenhouse. Spikes at 5, 10 and 15 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

text: Adult plant

text: E. coli SOLR

characteristic: lab-host

text: Triticum aestivum

text: EcoRI

text: XhoI

text: Spike

text: Wheat 5-15 DAP spike cDNA library

text: Phagemid

text: Lambda Uni-ZAP XR, excised phagemid


text: North Carolina State University

text: live

characteristic: INSDC status

text: Generic

text: Heterodera glycines

characteristic: strain

text: Heterodera glycines OP50


text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.8 kb. Library constructed by Life Technologies.

text: DH10B (phage-resistant)

text: colon

characteristic: organ

text: Homo sapiens

text: NotI

text: SalI

text: adenocarcinoma

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.75 kb. Library constructed by Life Technologies.

characteristic: description

text: DH10B (phage-resistant)

text: eye

text: Homo sapiens

text: NotI

text: SalI

text: retinoblastoma

characteristic: title

text: phagemid (ampicillin resistant)


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: DH10B (phage-resistant)

text: lung

text: Homo sapiens

text: NotI

text: SalI

text: large cell carcinoma

characteristic: tissue-type

text: phagemid (ampicillin resistant)

characteristic: vector


characteristic: External Id

text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.1 kb. Library constructed by Life Technologies.

text: DH10B (phage-resistant)

text: lung

text: Homo sapiens

characteristic: organism

text: NotI

text: SalI

text: large cell carcinoma, undifferentiated

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: DH10B (phage-resistant)

text: pancreas

text: Homo sapiens

text: NotI

text: SalI

text: epithelioid carcinoma

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 2 kb. Library constructed by Life Technologies.

text: DH10B (phage-resistant)

text: skin

text: Homo sapiens

text: NotI

text: SalI

text: melanotic melanoma

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: DH10B (phage-resistant)

text: ovary

text: Homo sapiens

text: NotI

text: SalI

text: adenocarcinoma

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 2.1 kb.

text: DH10B (phage-resistant)

text: uterus

text: Homo sapiens

text: NotI

text: SalI

text: leiomyosarcoma

text: phagemid (ampicillin resistant)


text: Beijing 306 hospital, Beijing

text: live

text: Generic

text: colon

text: Homo sapiens

text: EcoRI

text: EcoRI

text: colon carcinoma tissue cDNA library

text: plasmid

text: pT AdV


text: Washington University School of Medicine

text: live

text: Generic

text: Clark

characteristic: cultivar

text: The Clark NIL was constructed and seed was provided by Dr. J. Specht, University of Nebraska (Shoemaker and Specht, 1995). The cDNA library was constructed from mRNA isolated from whole seedlings of 1 week old greenhouse grown plants. Complementary DNA was synthesized from mRNA using a primer consisting of a poly(dT) sequence with a XhoI restriction site and a 3' anchor. EcoRI adapters were ligated to the blunt-ended cDNA fragments followed by XhoI digestion. The cDNA fragments were directionally cloned into the EcoRI-XhoI restriction site of the pBluescript vector. The ligated cDNA fragments were transformed into DH10B host cells (GibcoBRL). The library was constructed in cooperation with Dr. Paul Keim's laboratory at Northern Arizona University.

text: 1 week old

characteristic: dev-stage

text: Glycine max

text: EcoRI

text: XhoI

text: whole seedlings of greenhouse grown plants

text: plasmid

text: pBluescript II SK+


characteristic: INSDC center name

text: live

text: Generic

text: Clark

text: The Clark NIL was constructed and seed was provided by Dr. J. Specht, University of Nebraska (Shoemaker and Specht, 1995). The cDNA library was constructed from mRNA isolated from whole seedlings of 3 week old greenhouse grown plants. Complementary DNA was synthesized from mRNA using a primer consisting of a poly(dT) sequence with a XhoI restriction site and a 3' anchor. EcoRI adapters were ligated to the blunt-ended cDNA fragments followed by XhoI digestion. The cDNA fragments were directionally cloned into the EcoRI-XhoI restriction site of the pBluescript vector. The ligated cDNA fragments were transformed into DH10B host cells (GibcoBRL). The library was constructed in cooperation with Dr. Paul Keim's laboratory at Northern Arizona University.

text: 3 week old

text: Glycine max

text: EcoRI

text: XhoI

text: plasmid

text: pBluescript II SK+


text: Deutsches Krebsforschungszentrum

characteristic: INSDC first public

text: live

text: Generic

text: Homo sapiens

text: larynx epithelium

text: 3.4 (downregulated in larynx carcinoma)


text: Deutsches Krebsforschungszentrum

characteristic: INSDC last update

text: live

text: Generic

text: Homo sapiens

text: larynx carcinoma

text: 3.3 (upregulated in larynx carcinoma)


text: Erasmus University Rotterdam

text: live

text: Generic

text: Homo sapiens

text: Homo sapiens library (Heus HC)


text: Centre for Cellular and Molecular Biology

text: live

text: Generic

text: Mouse embryonal carcinoma cells

characteristic: cell type

characteristic: cell-line

text: Differentially expressed cDNA fragment was PCR amplified and ligated at EcoRV site of pMOS blue vector(Amersham)

text: Mus musculus

text: Hind III

text: Differentiating PCC4 cells

text: Plasmid

text: pMOS blue


text: The Rockefeller University

text: live

characteristic: INSDC status

text: Generic

text: syn: Phreatamoeba balamuthi

text: Mastigamoeba balamuthi

text: ATCC 30984

characteristic: strain

text: Mastigamoeba balamuthi lambda ZAP II Library


text: National Institute for Basic Biology

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Oryzias latipes

text: HNI

text: OLHNI cell line cDNA library (OLb)


text: live

text: Generic

text: liver

characteristic: organ

text: Platichthys flesus

text: Sfi IA

text: Sfi IB

text: Male

characteristic: sex

text: liver

text: European flounder liver cDNA library

text: Phage


text: Texas A&M University

text: live

text: Generic

text: vascular smooth muscle

text: cDNA was identified and isolated from Mus musculus benzo(a)pyrene-activated total RNA pool by Differential Display Polymerase Chain Reaction using indicated anchor and arbitrary primer (Hieroglyph mRNA Profile Kit 3, Genomyx Corporation, Foster City, CA) and directly sequenced from PCR products.

characteristic: description

text: Mus musculus

text: female

text: aorta

characteristic: tissue-type

text: 3uM, 8h BaP-treated total RNA (2)

characteristic: title


characteristic: External Id

text: Faculty of Veterinary medicine, Universite de Montreal

text: live

text: Generic

text: granulosa

text: library made from ovarian granulosa cells of dominant follicles collected at day 5 or day 6 of the estrous cycle

text: Bos taurus

characteristic: organism

text: female

text: mixed

text: ovarian follicle

text: Bos taurus granulosa cell cDNA Lambda Zap Express

text: pBK-CMV phagemid

characteristic: vector


text: University of Texas

characteristic: INSDC center name

text: live

text: Generic

text: Spore

text: Brogn

characteristic: cultivar

text: EST sequence from cDNA library. cDNA library constructed from mRNA isolated from C. richardii spores that had developed for 20 hours after their germination had been initiated by white light.

text: 20 hours after germination initiation

characteristic: dev-stage

text: Ceratopteris richardii

text: Gametophyte

text: Ceratopteris Spore Library

text: plasmid


text: Beijing Institute of Basic Medical

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: lymphoma cell

characteristic: cell type

text: Homo sapiens

text: EcoRI

text: EcoRI

text: lymphoma

text: U937 cells Express Library

text: Plasmid

text: pGEM-T Easy Vector


text: National University of Ireland Galway

text: live

characteristic: INSDC status

text: Generic

text: An SSH library was constructed using cDNA from high metabolic rate fish (Tester) and cDNA from low metabolic rate fish (Driver)

text: First Feeding Stage

text: Escherichia coli

characteristic: lab-host

text: Salvelinus alpinus

text: Olves

characteristic: strain

text: Arctic charr SSH Library

text: Plasmid

text: pGEM-T easy (Promega)


text: Kyoto University

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Bos taurus

text: Japanese Black

text: fetus

text: Cloned Japanese Black bovine fetus cDNA


text: Forestry and Forest Products Research Institute

text: live

text: Generic

text: Cryptomeria japonica

text: inner bark

text: Cryptomeria japonica inner bark


text: Texas A&M University

text: live

text: Generic

text: vascular smooth muscle

text: cDNA was identified and isolated from Mus musculus benzo(a)pyrene-downregulated total RNA pool by Differential Display Polymerase Chain Reaction using indicated anchor and arbitrary primer (Hieroglyph mRNA Profile Kit 3, Genomyx Corporation, Foster City, CA) and sequenced directly from PCR products.

characteristic: description

text: Mus musculus

text: female

characteristic: sex

text: aorta

text: 3uM, 8h BaP-treated total RNA (downregulated)


text: live

text: Generic

characteristic: cell-line

text: Anchor enzyme: NlaIII~ Control for SAGE_PTEN. Cells were infected with an adenovirus expressing the beta-galactosidase gene. Library construction by MicroSAGE adaptation of Velculescu, V. E., Zhang, L.,Vogelstein, B., and Kinzler, K. W. (1995) Serial Analysis of Gene Expression. Science 270, 484-487. Library constructed in the laboratory of Ramon Parsons M.D., Ph.D. (Columbia University)

text: Homo sapiens

text: breast

characteristic: tissue-type

characteristic: title

text: plasmid


characteristic: External Id

text: live

text: Generic

text: Anchor enzyme: NlaIII~ Human breast cancer cell line MDA-MB-468 that is PTEN-/-. Cells were infected with an adenovirus expressing the PTEN tumor suppressor.~ Library construction by MicroSAGE adaptation of Velculescu, V. E., Zhang, L.,Vogelstein, B., and Kinzler, K. W. (1995) Serial Analysis of Gene Expression. Science 270, 484-487. Library constructed in the laboratory of Ramon Parsons, M.D., Ph.D. (Columbia University)

text: Homo sapiens

characteristic: organism

text: breast

text: plasmid

characteristic: vector


text: live

text: Generic

text: glioblastoma

text: Anchor enzyme: NlaIII~ Brain, glioblastoma multiforme cell line, H54, that has been stably transfected with epidermal growth factor receptor vIII mutant~ Library construction by MicroSAGE adaptation of Velculescu, V. E., Zhang, L.,Vogelstein, B., and Kinzler, K. W. (1995) Serial Analysis of Gene Expression. Science 270, 484-487. Tissue supplier: Greg J. Riggins, Ph.D. Library constructed in the laboratory of G. Riggins, M.D., Ph.D. (Duke University).

text: Homo sapiens

text: brain

text: plasmid


text: live

text: Generic

text: glioblastoma

text: Anchor enzyme: NlaIII~ Brain, glioblastoma multiforme cell line, H54, that has been stably transfected with the b-galactosidase gene~ Library construction by MicroSAGE adaptation of Velculescu, V. E., Zhang, L.,Vogelstein, B., and Kinzler, K. W. (1995) Serial Analysis of Gene Expression. Science 270, 484-487. Tissue supplier: Greg J. Riggins, Ph.D. Library constructed in the laboratory of G. Riggins, M.D., Ph.D. (Duke University).

text: Homo sapiens

text: brain

text: plasmid


text: live

text: Generic

text: cancer cell line

text: Anchor enzyme: NlaIII~ Pancreas, cancer cell line~ Library construction by MicroSAGE adaptation of Velculescu, V. E., Zhang, L.,Vogelstein, B., and Kinzler, K. W. (1995) Serial Analysis of Gene Expression. Science 270, 484-487. Tissue supplier: ATCC Library constructed in the Kern laboratory

text: Homo sapiens

text: pancreas

text: plasmid


text: live

text: Generic

text: Anchor enzyme: NlaIII~ Pancreas, normal duct epithelial cells, short term culture~ Library construction by MicroSAGE adaptation of Velculescu, V. E., Zhang, L.,Vogelstein, B., and Kinzler, K. W. (1995) Serial Analysis of Gene Expression. Science 270, 484-487. Tissue supplier: M.A. Hollingsworth Library constructed in the Kern laboratory

text: Homo sapiens

text: pancreas

text: plasmid


text: live

text: Generic

text: Anchor enzyme: NlaIII~ Normal prostate tissue obtained by manual microdissection of frozen tissue.~ Library treatment: non-normalized~ Tissue supplier: Dr. Carolyn Best~ Sample type: Microdissected~ IDs of libraries from the same tissue sample: 14913~ Library constructed in the laboratory of G. Riggins, M.D., Ph.D. (Duke University).

text: prostate

characteristic: organ

text: Homo sapiens

text: male

text: plasmid


characteristic: INSDC first public

text: live

text: Generic

text: Anchor enzyme: NlaIII~ Prostate tumor tissue obtained by manual microdissection of frozen tissue.~ Library treatment: non-normalized~ Tissue supplier: Dr. Carolyn Best~ Sample type: Microdissected~ IDs of libraries from the same tissue sample: 14912~ Library constructed in the laboratory of G. Riggins, M.D., Ph.D. (Duke University).

text: prostate

text: Homo sapiens

text: male

text: plasmid


characteristic: INSDC last update

text: live

text: Generic

text: cancer cell line

text: Anchor enzyme: NlaIII~ Pancreas, cancer cell line~ Library construction by MicroSAGE adaptation of Velculescu, V. E., Zhang, L.,Vogelstein, B., and Kinzler, K. W. (1995) Serial Analysis of Gene Expression. Science 270, 484-487. Tissue supplier: ATCC~ Library constructed in the Kern laboratory

text: Homo sapiens

text: pancreas

text: plasmid


text: live

text: Generic

text: epithelium

characteristic: cell type

text: Anchor enzyme: NlaIII~ CGAP Lib id: 569~ Library treatment: non-normalized~ Tissue supplier: Pat J. Morin, Ph.D.~ Induced 293 cells, expressing beta-catenin.~ Library constructed in the laboratory of P. Morin, Ph.D. (National Institute on Aging).

text: kidney

text: Homo sapiens

text: plasmid


text: live

characteristic: INSDC status

text: Generic

text: Anchor enzyme: NlaIII~ Normal pancreatic duct epithelium cell line~ CGAP Lib id: 559~ Tissue supplier: M.A. Hollingsworth, as described in Pancreas 15:265-271 (1997)~ Library construction by MicroSAGE adaptation of Velculescu, V. E., Zhang, L.,Vogelstein, B., and Kinzler, K. W. (1995) Serial Analysis of Gene Expression. Science 270, 484-487. Library constructed in the Kern laboratory

text: Homo sapiens

text: pancreas

text: plasmid


text: Norman Bethune University of Medical Science

characteristic: INSDC center name

text: live

text: Generic

text: Cervus nippon

text: Chinese Shuangyang Sika Deer mRNA


text: Michigan State University

text: live

text: Generic

text: ESTs isolated from differential display experiment

text: day 21 fetus

characteristic: dev-stage

text: Sus scrofa

text: Pig 21 day fetal cDNA


text: University of Georgia

text: live

text: Generic

characteristic: NCBI submission model

text: Pinus elliottii

text: Pinus elliottii cDNA library


text: University of Georgia

text: live

text: Generic

characteristic: NCBI submission package

text: Cronartium quercuum f. sp. fusiforme

text: Cronartium quercuum f. sp. fusiforme cDNA library


text: University of Illinois

text: live

text: Generic

text: The library Gm-r1070 is a sequence-driven, reracked set of 9,216 clones selected from cDNA libraries from various tissues and stages of development of soybean that represent 2,639 sequences from immature cotyledons, 1,770 from immature seed coats, 3,938 from flowers, and 869 from young pods. The 5' ESTs of the source clones from the different libraries was used to select singletons, or a representative of each contig, which were reracked to form library Gm-r1070. The cDNA clones of the reracked Gm-r1070 library were then sequenced at the 3' end. The contig analysis to select unique genes was performed by the laboratory of Ernest Retzel, Center for Computational Genomics and Bioinformatics, University of Minnesota, http://www.cbc.umn.edu/ResearchProjects/Soybean/index.html. Reracking was performed by Genome Systems, St. Louis, http://www.genomesystems.com, and 3' sequencing by the Keck Center for Comparative and Functional Genomics, University of Illinois, http://www.life.uiuc.edu/biotech/keck.html. Note: The corresponding 5' EST from each clone in the Gm-r1070 library is listed in the 'OTHER EST' field. The detailed information on the source library for each clone can also be obtained by referring to the Genome Systems clone ID of the original cDNA library that is also listed under 'OTHER EST'.

characteristic: description

text: Glycine max


text: Agriculture and Agri-Food Canada

text: live

text: Generic

text: Harosoy 63

characteristic: cultivar

text: This cDNA library was constructed from polyA+ enriched mRNA from green seed coats in mid to late developemental stage, average fresh weight 250 mg per seed. Traces of pod and embryo tissue also present. Complementary DNA was synthesized from mRNA using an XhoI-poly(dT) linker-primer. EcoRI adopters were ligated to the blunt-ended cDNA fragments and the products were digested with XhoI for directional cloning into lambda ZAP Express vector. This lambda library was amplified once using E. coli host strain XL1 Blue MRF'. Inserts were then subcloned by mass excision using ExAssist helper phage for conversion into phagemid vector pBK-CMV in E. coli host strain XLOLR.

text: E. coli strain XLOLR

characteristic: lab-host

text: Glycine max

text: EcoRI

text: XhoI

text: Seed coats

text: GmaxSC

text: pBK-CMV


text: Tohoku University

text: live

text: Generic

text: young adult

text: Cavia porcellus

text: Hartley

characteristic: strain

text: organ of Corti

characteristic: tissue-type

text: Guinea pig organ of Corti pKF3 library


characteristic: External Id

text: Penn State University

text: live

text: Generic

text: Columbia

text: PCR Suppression Subtractive Hybridization library (Clontech PCR-Select). 'Tester' = leaf tissue from plants exposed to 1 hr 0.4 ul/L ozone. 'Driver' = leaf tissue from control plants maintained in clean air. Cloned into pCR2.1TOPO vector (Invitrogen).

text: Arabidopsis thaliana

text: Leaf

text: Arabidopsis Acute Ozone Forward-Subtracted Library

characteristic: title

text: Plasmid


text: Penn State University

text: live

text: Generic

text: Columbia

text: PCR Suppression Subtractive Hybridization library (Clontech PCR-Select). 'Tester' = leaf tissue from plants exposed to 1 hr 0.4 ul/L ozone. 'Driver' = leaf tissue from control plants maintained in clean air. Cloned into pT-Adv vector (Clontech).

text: Arabidopsis thaliana

characteristic: organism

text: Leaf

text: Arabidopsis Acute Ozone Reverse-Subtracted Library

text: Plasmid

text: pT-Adv

characteristic: vector


text: Penn State University

characteristic: INSDC first public

text: live

text: Generic

text: Columbia

text: PCR Suppression Subtractive Hybridization library (Clontech PCR-Select). 'Tester' = RNA pooled from leaf tissue of plants exposed to 3, 6, and 9 days of ozone at 0.15 ul/L for 6 h/d. 'Driver' = leaf tissue from control plants maintained in clean air. Cloned into 3.9 kB AdvanTAge vector (Clontech).

text: Arabidopsis thaliana

text: Leaf

text: Arabidopsis Chronic Ozone Forward-Subtracted Library

text: Plasmid

text: pT-Adv


text: National Institute of Health

characteristic: INSDC last update

text: live

text: Generic

text: Smooth muscle

text: Liver

characteristic: organ

text: Oncorhynchus mykiss

text: EcoRI

text: XhoI

text: Rainbow Trout Liver Lambda Zap II Vector library

text: Plasmid

text: pBluescript SK(+/-)


text: live

characteristic: INSDC status

text: Generic

text: cDNA made by oligo-dT priming. Directionally cloned using the following adaptors: 5'-TCGACCCACGCGTCCG-3' and 5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCT(15)-3'. Size selected > 1 kb for average insert length 1.7 kb. This is a primary library, non-amplified. Library constructed by Life Technologies and donated by J. Lupski, M.D./Ph.D. (Baylor College of Medicine) and is available through Life Technologies.

text: adult, 36 yr

text: Homo sapiens

text: NotI

text: SalI

text: male

characteristic: sex

text: dorsal root ganglia

text: plasmid (ampicillin resistant, 200 ug/ml)

text: pCMV-SPORT6 (Life Technologies)


text: live

text: Generic

text: mRNA made from thyroid carcinoma, cDNA made by oligo-dT priming. Non-directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 500 bp. Primary library. cDNA Library Preparation: David B. Krizman, Ph.D. | |

text: thyroid

text: Homo sapiens

text: follicular carcinoma

text: plasmid (ampicillin resistant)


text: Agriculture and Agri-food Canada

characteristic: INSDC center name

text: live

text: Generic

text: Puma (winter rye)

text: Sampled three-leaf seedlings treated for one week at 2oC, 12 hrs light/day. Library made with Stratagene UNIZAP XR Kit/Gigapack III Gold Kit. Lambda library is amplified, then mass excised in SOLR cells.

text: seedling three-leaf stage

characteristic: dev-stage

text: Secale cereale

text: Eco RI

text: Xho I

text: leaf, crown

text: Sc01_AAFC_ECORC_cold_stressed_winter_rye_seedlings

text: Plasmid

text: Bluescript SK-/XhoI-EcoRI


text: Agriculture and Agri-food Canada

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Puma (winter rye)

text: Sampled three-leaf seedlings treated for one week at 2oC, 12 hrs light/day. Library made with Stratagene UNIZAP XR Kit/(not packaged). cDNA is directly ligated into SK+/XhoI-EcoRI, then electroporated into TOP10 cells (Invitrogen).

text: seedling three-leaf stage

text: Secale cereale

text: Eco RI

text: Xho I

text: leaf, crown

text: Sc02_AAFC_ECORC_cold_stressed_winter_rye_seedlings

text: Plasmid

text: Bluescript SK+/XhoI-EcoRI


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: This library has been used successfully to clone a number of full-length cDNAs ranging in size from 1.4 to 4.5 kb. There are less than 0.5% clones with multiple inserts. Since each cDNA has an EcoRI site at its 5' end and an XhoI site at the 3' end, these clones can be easily identified. One should be suspicious of any clone which gives 3 or more bands in an EcoRI-XhoI double digest AND has an internal XhoI site. We usually do not further characterize any such clones unless the cDNA is known to give multiple bands in an EcoRI-XhoI digest. Microplate status: 500,000 unamplified cDNAs were mass excised (pBluescript SK-) in Xl1-Blue using ExAssist phage. The resulting single-stranded phagemids were used to infect Top10F'. Clones were picked into freezing medium (per liter 15 g tryptone, 10g yeast extract, 5g NaCl, 36 mM K2HPO4, 13.2 mM KH2PO4, 1.7 mM Na-citrate, 0.4 mM MgSO4*7 H2O, 6.8 mM (NH4)2SO4, 4% w/v glycerol) and grown for 24 hours. Original library contruction by Bruce Blumberg (Blumberg et al., 1991 Science 253, 194-196; Hawlet et al., 1995, Genes Dev. 9, 2923-2935). Note: This is a Xenopus Gene Collection (XGC) library

characteristic: description

text: Top-10 F'

text: Xenopus laevis

text: EcoRI

text: XhoI

text: unfertilized egg

text: Xenopus laevis unfertilized egg cDNA library

text: phagemid (ampicillin resistant)

text: pBluescript SK-


text: The University of Michigan Medical School

text: live

text: Generic

text: Transcripts identified in a cDNA subtraction screen.

text: Pituitary gland

text: Mus musculus

text: DF/B (Mixed background)

text: Mouse e14.5 developing pituitary gland

text: Plasmid

text: pGEMTeasy


text: Michigan State University

text: live

text: Generic

text: ESTs isolated from differential display experiment

text: day 45 fetus

text: Sus scrofa

text: Pig 45 day fetal cDNA


text: Michigan State University

text: live

text: Generic

text: day 35 fetus

text: Sus scrofa

text: Pig 35 day fetal cDNA


text: Ludwig Institute for Cancer Research

text: live

text: Generic

text: A mini-library was made by cloning products derived from ORESTES PCR (U.S. Letters Patent application No. 196,716 - Ludwig Institute for Cancer Research) profiles into the pUC 18 vector. Reverse transcription of tissue mRNA and cDNA amplification were performed under low stringency conditions.

text: Adult

text: breast

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: Ludwig Institute for Cancer Research

text: live

text: Generic

text: Adult

text: colon

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: Ludwig Institute for Cancer Research

text: live

text: Generic

text: Adult

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: Kyoto University

text: live

text: Generic

text: tailbud embryo

text: Ciona intestinalis

text: whole animal

text: Nori Satoh unpublished cDNA library


text: live

text: Generic

text: Double-stranded cDNA was prepared from cell line RNA. 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.65 kb (range 0.9-4.0 kb). 14/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA).

text: DH10B (T1 phage-resistant)

characteristic: lab-host

text: bone marrow

text: Homo sapiens

text: SfiI (ggccgcctcggcc)

text: SfiI (ggccattatggcc)

text: from acute myelogenous leukemia

text: plasmid (chloramphenicol resistant, grown at 30C)

text: pDNR-LIB (Clontech)


text: live

text: Generic

text: Double-stranded cDNA was prepared from cell line RNA. 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.55 kb (range 0.9-4.0 kb). 12/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA).

text: DH10B (T1 phage-resistant)

text: brain

text: Homo sapiens

text: SfiI (ggccgcctcggcc)

text: SfiI (ggccattatggcc)

text: glioblastoma

text: pDNR-LIB (Clontech)


text: live

text: Generic

text: Double-stranded cDNA was prepared from cell line RNA. 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.35 kb (range 0.9-4.0 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA).

text: DH10B (T1 phage-resistant)

text: kidney

text: Homo sapiens

text: SfiI (ggccgcctcggcc)

text: SfiI (ggccattatggcc)

text: hypernephroma

text: pDNR-LIB (Clontech)


text: The Biology Department of Shandong Normal University

text: live

text: Generic

text: total RNA extraction from NaCl(200mM) treated Thellungiella halophila by RNAgent kit(Promega); mRNA isolation by MESSAGEMAKER kit(GIBCO BRL); directional cDNA synthesis(EcoRI XhoI) by cDNA synthesis kit(STRATAGEN); the ZAP express library by GigapackIII Gold Cloning kit(STRATAGENE)

text: seedling

text: aerial part tissue

text: Eutrema halophilum

text: EcoRI

text: XhoI

text: Thellungiella halophila ZAP cDNA library

text: Phage

text: lambda zap


text: Universidade de Brasilia (UnB)

text: live

text: Generic

text: P. brasiliensis DD cDNA fragments cloned into pCR-Script vector

text: differentially expressed cDNAs during mycelium to yeast transition

text: Paracoccidioides brasiliensis

characteristic: strain

text: P. brasiliensis differentially expressed sequences


text: University of Wolverhampton

text: live

text: Generic

text: Druid

characteristic: cultivar

text: Beta vulgaris

text: young leaf

characteristic: tissue-type

text: Sugar beet sulphur induced mRNA library


characteristic: External Id

text: live

text: Generic

text: Directionally cloned using Stratagene's HybriZap-2.1 XR cDNA cloning kit with the 5' end at the EcoRI site. The library represents 1 x 10e6 independent recombinant phage. The plants were grown in a growth chamber with a 16 hour day and an 8 hour night at 25 degrees.

text: 10 days

text: shoot

text: Zea mays

text: EcoRI

text: XhoI

text: leaf

text: 948 - 10 day shoot from Barkan lab

characteristic: title

text: phagemid

text: HybriZap-2.1 (pAD-GAL4-2.1)


text: Stanford University

text: live

text: Generic

text: Directionally cloned using Stratagene's UniZap XR cDNA cloning kit with the 5' end at the EcoRI site. The library represents 8 x 10e5 independent recombinant phage. The plants were greenhouse grown.

text: 2 week old seedling (3 leaves)

text: shoot

text: Zea mays

characteristic: organism

text: EcoRI

text: XhoI

text: leaf and stem, including leaf base

text: 947 - 2 week shoot from Barkan lab

text: phagemid

text: Lambda ZAP (pBlueScript SK-)

characteristic: vector


text: Wageningen University

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Total RNA was isolated from mycelium of P. infestans DDR7602 cultured for 4 weeks in synthetic medium. EST clones were named by their position in the microtiter plate, preceded by the prefix MY (for mycelial) and the successive number of the microtiter plate (e.g. MY-06-A-04).

text: 4-week old vegetative, non-sporulating mycelium in synthetic medium

text: E. coli, strain DH5-alpha

text: Phytophthora infestans

text: SalI

text: NotI

text: DDR7602, A1 mating type

text: PinfestansMY

text: Plasmid


text: Washington University School of Medicine

text: live

characteristic: INSDC status

text: Generic

text: Clark

text: The Clark NIL was constructed and seed was provided by Dr. J. Specht, University of Nebraska (Shoemaker and Specht, 1995). The cDNA library was constructed from mRNA isolated from leaf tissue at various developmental stages of 3 week old greenhouse grown plants. Complementary DNA was synthesized from mRNA using a primer consisting of a poly(dT) sequence with a XhoI restriction site and a 3' anchor. EcoRI adapters were ligated to the blunt-ended cDNA fragments followed by XhoI digestion. The cDNA fragments were directionally cloned into the EcoRI-XhoI restriction site of the pBluescript vector. The ligated cDNA fragments were transformed into DH10B host cells (GibcoBRL). The library was constructed in cooperation with Dr. Paul Keim's laboratory at Northern Arizona University.

text: 3 week old

text: Glycine max

text: EcoRI

text: XhoI

text: leaf tissue at various developmental stages of greenhouse grown plants

text: plasmid

text: pBluescript II SK+


text: live

text: Generic

text: Corolla

text: The cDNA library was constructed from floral meristematic mRNA provided by Dr. Halina Knap of Clemson University. Complementary DNA was synthesized from mRNA using a primer consisting of a poly(dT) sequence with a XhoI restriction site. EcoRI adapters were ligated to the blunt-ended cDNA fragments followed by XhoI digestion. The cDNA fragments were directionally cloned into the EcoRI-XhoI restriction site of the pBluescript vector. The ligated cDNA fragments were transformed into DH10B host cells (GibcoBRL). This library was constructed in the laboratory of Dr. Randy Shoemaker.

text: Glycine max

text: EcoRI

text: XhoI

text: floral meristematic mRNA

text: plasmid

text: pBluescript II SK+


text: live

text: Generic

text: Harosoy

text: The Harosoy NIL was constructed and seed was provided by Dr. J. Specht, University of Nebraska (Shoemaker and Specht, 1995). The cDNA library was constructed from mRNA isolated from whole seedlings of 1 week old greenhouse grown plants. Complementary DNA was synthesized from mRNA using a primer consisting of a poly(dT) sequence with a XhoI restriction site and a 3' anchor. EcoRI adapters were ligated to the blunt-ended cDNA fragments followed by XhoI digestion. The cDNA fragments were directionally cloned into the EcoRI-XhoI restriction site of the pBluescript vector. The ligated cDNA fragments were transformed into DH10B host cells (GibcoBRL). The library was constructed in cooperation with Dr. Paul Keim's laboratory at Northern Arizona University.

text: 1 week old

text: Glycine max

text: EcoRI

text: XhoI

text: whole seedlings of greenhouse grown plants

text: plasmid

text: pBluescript II SK+


text: live

text: Generic

text: Raiden

text: The cDNA library was constructed from mRNA isolated from mature flowers of field grown plants for the cultivar Raiden. Complementary DNA was synthesized from mRNA using a primer consisting of a poly(dT) sequence with a XhoI restriction site. EcoRI adapters were ligated to the blunt-ended cDNA fragments followed by XhoI digestion. The cDNA fragments were directionally cloned into the EcoRI-XhoI restriction site of the pBluescript vector. The ligated cDNA fragments were transformed into DH10B host cells (GibcoBRL). This library was constructed in the laboratory of Dr. Randy Shoemaker.

text: Glycine max

text: EcoRI

text: XhoI

text: mature flowers of field grown plants

text: plasmid

text: pBluescript II SK+


text: live

text: Generic

text: Raiden

text: The cDNA library was constructed from mRNA isolated from stem tissue of 1 month old greenhouse grown plants for the cultivar Raiden. Complementary DNA was synthesized from mRNA using a primer consisting of a poly(dT) sequence with a XhoI restriction site. EcoRI adapters were ligated to the blunt-ended cDNA fragments followed by XhoI digestion. The cDNA fragments were directionally cloned into the EcoRI-XhoI restriction site of the pBluescript vector. The ligated cDNA fragments were transformed into DH10B host cells (GibcoBRL). This library was constructed in the laboratory of Dr. Randy Shoemaker.

text: 1 month old

text: Glycine max

text: EcoRI

text: XhoI

text: stem tissue of greenhouse grown plants

text: plasmid

text: pBluescript II SK+


text: live

text: Generic

text: Williams

text: This cDNA library was constructed from mRNA isolated from immature leaves (unfurled trifoliate) of greenhouse grown plants that were 2 week old. The library was prepared using the Life Technologies pSuperScript cDNA library construction kit. Complementary DNA was synthesized from mRNA using a poly(dT) sequence with a NotI restrictions site. SalI linkers adapters were ligated to the blunt-ended cDNA fragments followed by NotI digestion. The cDNA fragments were directionally cloned into the NotI-SalI restriction site of the pSPORT1 vector. The ligated cDNA fragments were transformed into E.coli ElectroMax DH10B host cells. This library was constructed in the laboratory of Dr. Lila Vodkin by Anu Khanna at the University of Illinois at Urbana-Champaign (email l-vodkin@uiuc.edu)

text: 2 week old

text: Glycine max

text: NotI

text: SalI

text: plasmid


text: Ludwig Institute for Cancer Research

text: live

text: Generic

text: A mini-library was made by cloning products derived from ORESTES PCR (U.S. Letters Patent application No. 196,716 - Ludwig Institute for Cancer Research) profiles into the pUC 18 vector. Reverse transcription of tissue mRNA and cDNA amplification were performed under low stringency conditions.

text: Adult

text: pnet

characteristic: organ

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: Ludwig Institute for Cancer Research

characteristic: INSDC center name

text: live

text: Generic

text: Adult

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: National Institute on Aging/National Institutes of Health

text: live

text: Generic

text: Total RNAs were extracted from 3000 Dopamine cells (cell collected by Dr.Tanya Barrett). The double-stranded cDNA was synthesized by Gibco's kit with an Oligo(dT) primer [NotI primer-adapter from GibcoBRL] [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 0.91ug of total RNA. The double-stranded cDNAs were treated with T4 DNA polymerase and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4 (include Sal1 sequence). The cDNAs were purified by phenol/chloroform and separated from free linkers by Centricon 100. Then, cDNAs were amplified by long-range high fidelity PCR using Takara's Ex Taq polymerase. Then, the cDNAs were purified by phenol/chloroform and by Centricon 100. The cDNAs were digested with SalI and NotI enzymes. Then, the cDNAs were size selected by Gibco's Size Fractionation Column. The cDNAs were cloned into SalI/NotI site of pSPORT1 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by chemical method. The library was constructed by Yulan Piao and Minoru.S. H. Ko.

characteristic: dev-stage

text: Mus musculus

text: SalI

text: NotI

text: Male/Female

characteristic: sex

text: TH-beta-gal transgenic mouse

text: NIA Mouse E13.5 VMB Dopamine cell cDNA Library

text: plasmid (ampicillin resistant)

text: pSPORT1 (Gibco/BRL Life Technology)


text: New York University School of Medicine

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: immune-responsive

text: adult

text: Anopheles gambiae

text: Anopheles gambiae G3 adult


text: Beijing University

text: live

text: Generic

text: kidney

text: Homo sapiens

text: Human kidney suppression subtractive hybridization


text: University of Illinois at Urbana-Champaign

text: live

text: Generic

text: The library was prepared by Stratagene using oligo-T and random priming and bidirectional cloning with an adaptor (GGCACGAG) on each end (although not all clones have the expected adaptor sequence after the EcoRI site). Many of the clones have multiple insertions, and where those are known their chimeric nature is noted in the EST description. The mRNA was prepared from antennae of adult female honey bees. Clones were subcloned in vivo in mass into pBluescript maintained in SOLR cells for DNA sequencing.

characteristic: description

text: adults

text: XL1 Blue MRF' and SOLR

characteristic: lab-host

text: antennae

text: Apis mellifera

text: EcoRI

text: female

text: Apis mellifera female antennae Lambda ZAP II library

text: lambda virus

text: Lambda ZAP II


text: live

text: Generic

text: The library was prepared by Stratagene using oligo-T and random priming and bidirectional cloning with an adaptor (GGCACGAG) on each end (although not all clones have the expected adaptor sequence after the EcoRI site). Many of the clones have multiple insertions, and where those are known their chimeric nature is noted in the EST description. The mRNA was prepared from antennae of adult male honey bees. Clones were subcloned in vivo in mass into pBluescript maintained in SOLR cells for DNA sequencing.

text: adults

text: XL1 Blue MRF' and SOLR

text: antennae

text: Apis mellifera

text: EcoRI

text: male

text: Apis mellifera male antennae Lambda ZAP II library

text: lambda virus

text: Lambda ZAP II


text: Ludwig Institute for Cancer Research

text: live

text: Generic

text: Adult

text: marrow

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: Ludwig Institute for Cancer Research

text: live

text: Generic

text: Adult

text: ovary

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: University of Nevada

text: live

text: Generic

text: AD Arabidopsis thaliana library enriched for salt-induced transcripts from wildtype (Col-0 gl1) 10-14 day-old seedlings treated with 160 mM NaCl for 4 hours.

text: 10-14 day old

text: Escherichia coli, TOP10F'

text: whole seedlings

text: Arabidopsis thaliana

text: EcoRI

text: EcoRI

text: ecotype Columbia gl1

characteristic: strain

text: mixed leaf and root

text: AD A. thaliana (Col-0 gl1) library enriched for salt-induced transcripts; 10-14 day seedlings; 4h 160mM NaCl stress

text: Plasmid

text: pT-Adv (CLONTECH)


text: University of Nevada

text: live

text: Generic

text: AD Arabidopsis thaliana subtracted library enriched for salt-induced transcripts from wildtype (Col-0 gl1) 10-14 day-old seedlings treated with 160 mM NaCl for hours.

text: 10-14 day old

text: Escherichia coli, TOP10F'

text: whole seedlings

text: Arabidopsis thaliana

text: EcoRI

text: EcoRI

text: ecotype Columbia gl1

text: mixed leaf and root

characteristic: tissue-type

text: AD A. thaliana (Col-0 gl1) subtracted library enriched for salt-induced transcripts;10-14 day seedlings 4h 160mM NaCl stress

text: Plasmid

text: pT-Adv (CLONTECH)


characteristic: External Id

text: Korea University, College of Medicine

text: live

text: Generic

text: average insert size: 300 bp; ordered differential display PCR product

text: embryonal stage(E12,14,16,18,20) or postnatal stage(P0,P3,P7,P12,P25) or adult

text: Top 10

text: Rattus norvegicus

text: brain

text: rat brain

text: pGEM T-easy


text: Academic Medical Center

text: live

text: Generic

text: melanocytic cells

characteristic: cell type

text: Homo sapiens

text: skin

text: Homo sapiens skin melanocytic cells

characteristic: title


text: Miyazaki University

text: live

text: Generic

text: Seriola quinqueradiata

characteristic: organism

text: Seriola quinqueradiata mRNA


text: Osaka University

characteristic: INSDC first public

text: live

text: Generic

text: HeLa

characteristic: cell-line

text: Homo sapiens

text: Homo sapiens HeLa


text: Medical University of South Carolina

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Hemocyte

text: Penaeus setiferus

text: Sfi I

text: Sfi I

text: Hemolymph

text: Plasmid

characteristic: vector


text: Chinese National Human Genome Center at Shanghai

characteristic: INSDC center name

text: live

text: Generic

text: Adult

characteristic: dev-stage

text: SOLR

text: Homo sapiens

text: EcoRI

text: XhoI

text: hepatocellular carcinoma

text: GKA

text: pBluescript sk(-)


text: live

text: Generic

characteristic: NCBI submission model

text: Adult

text: SOLR

text: Homo sapiens

text: EcoRI

text: XhoI

text: hepatocellular carcinoma

text: GKB

text: pBluescript sk(-)


text: live

text: Generic

characteristic: NCBI submission package

text: Adult

text: SOLR

text: Homo sapiens

text: EcoRI

text: XhoI

text: hepatocellular carcinoma

text: GKC

text: pBluescript sk(-)


text: live

text: Generic

text: Adult

text: SOLR

text: Homo sapiens

text: EcoRI

text: XhoI

text: hepatocellular carcinoma

text: GKD

text: pBluescript sk(-)


text: Cedars-Sinai Medical Center

text: live

text: Generic

text: Myeloid

text: Representation difference PCR analysis (Clontech RDA kit) of myeloid cells from thioglycollate-challenged C/EBP-epsilon knockout mice versus the wild-type. Cloned into pCR2.1 TA vector (Invitrogen).

characteristic: description

text: 1 month old

text: Mus musculus

text: male and female

characteristic: sex

text: 129/SvEv x NIH Swiss

text: Peritoneal exudate

text: RDA-C/EBPE

text: Plasmid


text: Lawrence Livermore National Laboratory

text: live

text: Generic

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCCTTAATTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library went through one round of normalization. Library constructed by Bento Soares and M. Fatima Bonaldo.

text: DH10B (phage-resistant)

characteristic: lab-host

text: Mus musculus

text: NotI

text: EcoRI

text: mandible

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCCGCGCTTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library went through one round of normalization. Library constructed by Bento Soares and M. Fatima Bonaldo.

text: DH10B (phage-resistant)

text: Mus musculus

text: NotI

text: EcoRI

text: maxillary process

characteristic: tissue-type

text: phagemid (ampicillin resistant)


characteristic: External Id

text: West Virginia University

text: live

text: Generic

text: Smooth muscle

text: Kidney

characteristic: organ

text: Oncorhynchus mykiss

text: EcoRI

text: Xho I

text: Rainbow Trout Kidney Lambda Zap II Vector library

text: Plasmid

text: pBluescript SK(+/-)


text: Oregon State University

text: live

text: Generic

text: cDNA prepared from 2, 14, 24 hrs post-fertilization, mixed, and cloned into pJG4-5 (two-hybrid 'prey' vector)

text: Zygote, 2-24 hrs post-fertilization

text: S. cerevisiae, E. coli

text: Fucus distichus

text: EcoRI

text: XhoI

text: Fucus zygote two-hybrid library

characteristic: title

text: Plasmid


text: Dept of AFNS, University of Alberta

text: live

text: Generic

text: Adipocyte

characteristic: cell type

text: Young adult

text: Subcutaneous, Omental, Mesenteric adipose

text: Bos taurus

characteristic: organism

text: EcoRI

text: Xho I

text: two males and one female mixed

text: Adipose

text: Bovine Mixed Adipose cDNA library

text: Phage


text: Fourth Military Medical University

characteristic: INSDC first public

text: live

text: Generic

text: Homo sapiens

text: Homo sapiens rheumatoid arthritis fibroblast-like synovial cells


text: Biology, University of New Mexico

characteristic: INSDC last update

text: live

text: Generic

text: Botryllus schlosseri

text: Botryllus schlosseri EST


text: Biology, University of New Mexico

text: live

characteristic: INSDC status

text: Generic

text: midcycle

text: Botryllus schlosseri

text: Botryllus schlosseri midcycle


text: Clemson University

text: live

text: Generic

text: prepared by suppression subtractive hybridization using root mRNA, 10 hours after soybean cyst nematode infection as a tester. The non-infected root mRNA served as a driver.

text: ten hours after soybean cyst nematode infection

text: Glycine max

text: PI 437654

characteristic: strain

text: root

text: soybean root subtractive cDNA Library

text: pGEM-T

characteristic: vector


text: USDA ARS PSI

characteristic: INSDC center name

text: live

text: Generic

characteristic: cultivar

text: Soybean (Glycine max) cv. Peking(PI548.402) cDNA library made from sterile leaf and root tissue infected for two days with soybean cyst nematodes (Heterodera glycines)

text: Glycine max

text: Soybean, Heterodera glycines infected, cDNA library

text: Plasmid

text: pBluescript


text: The Institute for Genomic Research (TIGR; www.tigr.org)

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Kennebec

text: Tissue was supplied by Dr. Fry (Cornell University). Leaflets and petioles were isolated from 8 week old greenhouse grown plants. The plants were watered and fertilized freely. The tissue was immediately frozen in liquid nitrogen.

text: 8 weeks old plants

characteristic: dev-stage

text: SOLR

text: Solanum tuberosum

text: leaflets and petioles

text: potato leaves and petioles

text: phagemid

text: pBlueScript SK(-)


text: Ludwig Institute for Cancer Research

text: live

text: Generic

text: A mini-library was made by cloning products derived from ORESTES PCR (U.S. Letters Patent application No. 196,716 - Ludwig Institute for Cancer Research) profiles into the pUC 18 vector. Reverse transcription of tissue mRNA and cDNA amplification were performed under low stringency conditions.

characteristic: description

text: Adult

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: Ludwig Institute for Cancer Research

text: live

text: Generic

text: Adult

text: breast

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: Ludwig Institute for Cancer Research

text: live

text: Generic

text: Adult

text: colon

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: Ludwig Institute for Cancer Research

text: live

text: Generic

text: Adult

text: marrow

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: Ludwig Institute for Cancer Research

text: live

text: Generic

text: Adult

text: stomach

text: Homo sapiens

text: SmaI

text: SmaI

text: Plasmid


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: Double-stranded cDNA was prepared from cell line RNA. 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.65 kb (range 0.9-4.0 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA).

text: DH10B (T1 phage-resistant)

characteristic: lab-host

text: brain

text: Homo sapiens

text: SfiI (ggccgcctcggcc)

text: SfiI (ggccattatggcc)

text: primitive neuroectoderm

characteristic: tissue-type

text: plasmid (chloramphenicol resistant, grown at 30C)

text: pDNR-LIB (Clontech)


characteristic: External Id

text: live

text: Generic

text: Double-stranded cDNA was prepared from cell line RNA. 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.75 kb (range 0.9-4.0 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA).

text: DH10B (T1 phage-resistant)

text: skin

characteristic: organ

text: Homo sapiens

text: SfiI (ggccgcctcggcc)

text: SfiI (ggccattatggcc)

text: melanotic melanoma, high MDR

text: pDNR-LIB (Clontech)


text: live

text: Generic

text: 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.35 kb (range 0.5-4.0 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA).

text: DH10B (T1 phage-resistant)

text: brain

text: Homo sapiens

text: SfiI (ggccgcctcggcc)

text: SfiI (ggccattatggcc)

characteristic: title

text: pDNR-LIB (Clontech)


text: live

text: Generic

text: 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.25 kb (range 0.6-4.0 kb). 14/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA).

text: DH10B (T1 phage-resistant)

text: heart

text: Homo sapiens

characteristic: organism

text: : SfiI (ggccgcctcggcc)

text: SfiI (ggccattatggcc)

text: pDNR-LIB (Clontech)


characteristic: INSDC first public

text: live

text: Generic

text: 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.2 kb (range 0.5-4.0 kb). 14/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA).

text: DH10B (T1 phage-resistant)

text: pancreas

text: Homo sapiens

text: SfiI (ggccgcctcggcc)

text: SfiI (ggccattatggcc)

text: pDNR-LIB (Clontech)


characteristic: INSDC last update

text: live

text: Generic

text: 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.55 kb (range 1.0-4.0 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA).

text: DH10B (T1 phage-resistant)

text: muscle (skeletal)

text: Homo sapiens

text: SfiI (ggccgcctcggcc)

text: SfiI (ggccattatggcc)

text: pDNR-LIB (Clontech)


text: live

characteristic: INSDC status

text: Generic

text: 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.35 kb (range 0.9-4.0 kb). 14/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA).

text: DH10B (T1 phage-resistant)

text: testis

text: Homo sapiens

text: SfiI (ggccgcctcggcc)

text: SfiI (ggccattatggcc)

text: pDNR-LIB (Clontech)


text: live

text: Generic

text: 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.4 kb (range 0.5-4.0 kb). 14/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA).

text: DH10B (T1 phage-resistant)

text: prostate

text: Homo sapiens

text: SfiI (ggccgcctcggcc)

text: SfiI (ggccattatggcc)

text: pDNR-LIB (Clontech)


text: M. S. Swaminathan Research Foundation

text: live

text: Generic

text: A one-year-old plant from the natural mangrove habitat in Pichavaram, Tamil Nadu, India was collected and brought to the M.S.Swaminatan Research Foundation. The whole plant was treated with the nutrient solution supplemented with 500 mM NaCl for 48 hours. Poly(A+)mRNA from the leaf tissue was purified and cDNA was prepared using Superscript Lambda System (Life Technologies, Cat. No. 19643-014). The cDNAs were size fractionated over SizeSep-400 spun column (Amersham-Pharmacia Biotech Cat No. 27-5105-01) and cloned in 5'Sal I - 3' Not I of pSPORT1 (Life Technologies Cat. No. 15383-011). The ESTs were sequenced from the 5' end using M13/pUC18 reverse primer in an Automated Sequencer (ABI310, Applied Biosystems) and submitted after editing to remove the vector and adapter sequences. BLAST search in BLASTX (Non-redundant)was carried out on 7th August, 2000 using default parameters and the results reported under putative identification/comment

text: 1 year old plant

text: Avicennia marina

text: Hermaphrodite

characteristic: sex

text: Pichavaram

characteristic: strain

text: Leaf

text: Avicennia marina leaf cDNA Library


text: College of Crop Science

characteristic: INSDC center name

text: live

text: Generic

text: Oryza sativa

text: root

text: Oryza sativa root (Yu_Futong)


text: University of Georgia

text: live

text: Generic

characteristic: cultivar

text: cDNA was prepared from polyA+ enriched RNA. The cDNA was directionally ligated into the Unizap XR vector from Stratagene and packaged using Gigapack III Gold packaging extracts. Plasmids containing cDNA inserts were excised from the recombinant lambda-Zap phage using Ex-assist helper phage and propagated in XLOLR cells.

text: 2 days after treatment with beta glucan elicitor preparation from Phytophthora sojae

text: E. coli strain XLOLR

text: Medicago truncatula

text: EcoRI

text: XhoI

text: Roots from four day old seedlings

text: MGHG

text: phagemid

text: pBluescript SK-

characteristic: vector


text: National Institute of Mental Health

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: The NIH_BMAP_MHI2 library is derived from mouse hippocampus tissue. For a detailed description of the library from which this clone was derived, please visit our web site at brainest.eng.uiowa.edu.

text: 27-32 days

text: DH10B (Life Technologies)

text: Mus musculus

text: Not I

text: Eco RI

text: Phagemid


text: National Institute of Mental Health

text: live

text: Generic

text: The NIH_BMAP_M_S5 library is a subtracted library of a series, ultimately derived from a mixture of individually tagged normalized libraries from ten regions of the mouse brain (cerebellum, brain stems, olfactory bulbs, hypothalamus, cortex, amygdala, basal ganglia, pineal gland, striatum, hipoccampus) after a series of subtractions to reduce the representation of cDNAs from which ESTs had already been generated. For a detailed description of the library from which this clone was derived, please visit our web site at brainest.eng.uiowa.edu.

characteristic: description

text: 27-32 days

characteristic: dev-stage

text: DH10B (Life Technologies)

text: Mus musculus

text: Not I

text: Eco RI

text: Phagemid


text: National Institute of Mental Health

text: live

text: Generic

text: The NIH_BMAP_Ret1 library is derived from mouse embryonic retina tissue. For a detailed description of the library from which this clone was derived, please visit our web site at brainest.eng.uiowa.edu. The tissue for this library was contributed by Dr. Xin-Yuan Fu, Yale University School of Medicine

text: 13.5 days pc

text: DH10B (Life Technologies)

characteristic: lab-host

text: Mus musculus

text: Not I

text: Eco RI

text: Phagemid


characteristic: External Id

text: National Institute of Mental Health

text: live

text: Generic

text: The NIH_BMAP_Ret1_N library is a normalized library derived NIH_BMAP_Ret1. NIH_BMAP_Ret1 was made from mouse embryonic retina tissue. For a detailed description of the library from which this clone was derived, please visit our web site at brainest.eng.uiowa.edu. The tissue for this library was contributed by Dr. Xin-Yuan Fu, Yale University School of Medicine

text: 13.5 days pc

text: DH10B (Life Technologies)

text: Mus musculus

text: Not I

text: Eco RI

text: Phagemid


text: National Institute of Mental Health

text: live

text: Generic

text: The NIH_BMAP_Ret2 library is derived from mouse neonatal retina tissue. For a detailed description of the library from which this clone was derived, please visit our web site at brainest.eng.uiowa.edu. The tissue for this library was contributed by Dr. Xin-Yuan Fu, Yale University School of Medicine

text: 1 day

text: DH10B (Life Technologies)

text: Mus musculus

text: Not I

text: Eco RI

text: Phagemid


text: National Institute of Mental Health

text: live

text: Generic

text: The NIH_BMAP_Ret2_N library is a normalized library derived NIH_BMAP_Ret2. NIH_BMAP_Ret2 was made from mouse neonatal retina tissue. For a detailed description of the library from which this clone was derived, please visit our web site at brainest.eng.uiowa.edu. The tissue for this library was contributed by Dr. Xin-Yuan Fu, Yale University School of Medicine

text: 1 day

text: DH10B (Life Technologies)

text: Mus musculus

text: Not I

text: Eco RI

text: Phagemid


text: National Institute of Mental Health

text: live

text: Generic

text: The NIH_BMAP_Ret3 library is derived from mouse retina tissue. For a detailed description of the library from which this clone was derived, please visit our web site at brainest.eng.uiowa.edu. The tissue for this library was contributed by Dr. Xin-Yuan Fu, Yale University School of Medicine

text: 6 weeks

text: DH10B (Life Technologies)

text: Mus musculus

text: Not I

text: Eco RI

text: Phagemid


text: National Institute of Mental Health

text: live

text: Generic

text: The NIH_BMAP_Ret3_N library is a normalized library derived NIH_BMAP_Ret3. NIH_BMAP_Ret3 was made from mouse retina tissue. For a detailed description of the library from which this clone was derived, please visit our web site at brainest.eng.uiowa.edu. The tissue for this library was contributed by Dr. Xin-Yuan Fu, Yale University School of Medicine

text: 6 weeks

text: DH10B (Life Technologies)

text: Mus musculus

text: Not I

text: Eco RI

text: Phagemid


text: National Institute of Mental Health

text: live

text: Generic

text: The NIH_BMAP_MSC_S1 library is a subtracted library derived NIH_BMAP_MSC_N. NIH_BMAP_MSC_N was made from mouse spinal cord tissue. For a detailed description of the library from which this clone was derived, please visit our web site at brainest.eng.uiowa.edu.

text: 27-32 days

text: DH10B (Life Technologies)

text: Mus musculus

text: Not I

text: Eco RI

text: Phagemid


text: Biomedical Research Institute

text: live

text: Generic

text: Hemocyte

characteristic: cell type

text: Total RNA was isolated from the hemocytes of unexposed and exposed Biomphalaria glabrata (BS-90) snails and first strand cDNA synthesized using anchored oligo (dT11-CG) primers (Operon Technologies, Alameda, CA). Radioactively labelled second strand synthesis was carried out using one of twelve 10-mer arbitrary primers (Operon Technologies, Alameda, CA)in a PCR based amplification reaction. The labelled PCR products were separated using 8% polyacrylamide/8M urea gels and the differentially expressed fragments identified by autoradiography. The different bands of interest were sliced out from the gel and the DNA solubilized and precipitated. This DNA was reamplified using the anchored oligo dT-arbitrary primer combination and the product cloned into the plasmid vector pCRII/TOPO. The differential display products were all cloned in a similar fashion and then subjected to sequencing.

text: Biomphalaria glabrata

text: EcoRI

text: hermaphrodite

text: Biomphalaria glabrata (BS90) differential display

text: plasmid

text: pCRII/TOPO


text: Shanghai Institute of Animal Parasitology

text: live

text: Generic

text: Schistosoma japonicum adult worms were obtained by perfusing from venae mesentery of rabbits experimentedly infected with Schistosoma japonicum cercariae (Anhui strain) for 42 days. Double-strand cDNA was synthesized with the mRNA isolated from Schistosoma japonicum adult worms and then was inserted into the Lambda ZipLox , Not I/ Sal I arms. The cDNA library was constructed by Key Lab of Animal Parasitology, Ministery of Agriculture, P.R. China. ( see: Yuan Chun-xiu et al. Chinese Journal of Biotechnology, 2000, 16(6): 80-83 )

text: adult worm

text: Schistosoma japonicum

text: NotI

text: SalI

text: mix

text: Anhui strain

text: Schistosoma japonicum (Anhui strain) cDNA Lambda ZipLox expression library

characteristic: title

text: Lambda ZipLox


text: Centro de Investigacion Cientifica de Yucatan

text: live

text: Generic

text: Coffea arabica

characteristic: organism

text: Coffea arabica mRNA


text: National Institute of Mental Health

characteristic: INSDC first public

text: live

text: Generic

text: The NIH_BMAP_Ret4_S1 library is a subtracted library, ultimately derived from mouse retina tissue libraries at various stages of development. For a detailed description of the library from which this clone was derived, please visit our web site at brainest.eng.uiowa.edu. The tissue for this library was contributed by Dr. Xin-Yuan Fu, Yale University School of Medicine

text: DH10B (Life Technologies)

text: Mus musculus

text: Not I

text: Eco RI

text: Phagemid


text: National Institute of Mental Health

characteristic: INSDC last update

text: live

text: Generic

text: The NIH_BMAP_Ret4_S2 library is a subtracted library, ultimately derived from mouse retina tissue libraries at various stages of development. For a detailed description of the library from which this clone was derived, please visit our web site at brainest.eng.uiowa.edu. The tissue for this library was contributed by Dr. Xin-Yuan Fu, Yale University School of Medicine

text: DH10B (Life Technologies)

text: Mus musculus

text: Not I

text: Eco RI

text: Phagemid


text: National Institute of Mental Health

text: live

characteristic: INSDC status

text: Generic

text: The NIH_BMAP_MHI2_S1 library is a subtracted library derived from NIH_BMAP_MHI2. NIH_BMAP_MHI2 is a library derived from mouse hippocampus tissue. For a detailed description of the library from which this clone was derived, please visit our web site at brainest.eng.uiowa.edu.

text: 27-32 days

text: DH10B (Life Technologies)

text: Mus musculus

text: Not I

text: Eco RI

text: Phagemid


text: Elanco Animal Health, A Division of Eli Lilly and Company

text: live

text: Generic

text: larva

text: Amblyomma americanum

text: EcoRI

text: XhoI

text: Male, Female

characteristic: sex

text: Amblyomma americanum larva Lambda Zap Express

text: Plasmid

text: pBK-CMV


text: live

text: Generic

text: Adult

text: Amblyomma americanum

text: EcoRI

text: XhoI

text: Male, Female

text: Amblyomma americanum adult Lambda Zap Express

text: Plasmid

text: pBK-CMV