text: Sericulture Research Institute, Chinese Academy of Agriculture Sciences

text: live

characteristic: INSDC status

text: Generic

text: the fifth instar larvae

text: Bombyx mori

text: mixed

characteristic: sex

characteristic: strain

text: midgut

text: SSH cDNA library of bombyx mori infected with cytoplasmic polyhedrosis virus

text: PGEM-T


status: PUBLIC

characteristic: External Id

text: West Bengal University of Technology

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Typha angustifolia

characteristic: organism

text: Jaduguda vs Kolkata

tag: attribute

text: Shoot

characteristic: tissue-type

text: DD Jaduguda vs Kolkata Typha angustifolia shoot

characteristic: title

text: Plasmid

text: pJET

characteristic: vector


text: Plant Genome Center Co.,Ltd

text: live

text: Generic

text: Ayamurasaki

characteristic: cultivar

text: developing

characteristic: dev-stage

text: Ipomoea batatas

text: tuberous root

text: sweet potato (Ayamurasaki) developing tuberous root cDNA


text: Plant Genome Center Co.,Ltd

text: live

text: Generic

text: Ayamurasaki

text: mature

text: Ipomoea batatas

text: tuberous root

text: sweet potato (Ayamurasaki) mature tuberous root cDNA


text: Plant Genome Center Co.,Ltd

text: live

text: Generic

text: mature

text: Ipomoea batatas

text: tuberous root

text: sweet potato (Kokei-14) mature tuberous root cDNA


text: Institute of Marine Biology and Genetics, Hellenic Center of Marine Research

text: live

text: Generic

text: cDNA library was constructed with the Creator SMART library construction kit (BD Bioscience-Clontech, Mountain View, Canada) using the LD PCR based method. Following the manufactures' instructions total RNA was used and between 20 and 22 PCR cycles were performed before size separation of inserts.

characteristic: description

text: kidney

characteristic: organ

text: Dicentrarchus labrax

text: Dicentrarchus labrax head kidney infected with Nodavirus

text: pDNR-lib vector


text: live

characteristic: INSDC status

text: Generic

text: brain

text: Dicentrarchus labrax

text: Dicentrarchus labrax brain infected with Nodavirus

text: pDNR-lib vector


text: live

text: Generic

text: Liver

text: Dicentrarchus labrax

text: Dicentrarchus labrax liver infected with Nodavirus

text: pDNR-lib vector


text: live

text: Generic

text: Spleen

text: Dicentrarchus labrax

text: Dicentrarchus labrax spleen infected with Nodavirus

text: pDNR-lib vector


text: Ceres, Inc

text: live

text: Generic

text: Zea mays

text: Mixed male/female infl., root


text: Ceres, Inc

text: live

text: Generic

text: 20 DAP

text: Zea mays

text: embryo


text: Ceres, Inc

text: live

text: Generic

text: Zea mays

text: root and shoot


text: Ceres, Inc

text: live

text: Generic

text: Zea mays

text: female flower


text: Ceres, Inc

text: live

text: Generic

text: Zea mays

text: Immature M/F flower, kernel, embryo


text: Ceres, Inc

text: live

text: Generic

text: Zea mays

text: callus


text: live

text: Generic

text: Zea mays

text: Immature M/F flower, kernel, embryo


text: Ceres, Inc

text: live

text: Generic

text: 20 DAP

text: Zea mays

text: embryo

text: CERES-CN


text: Ceres, Inc

text: live

text: Generic

text: Zea mays

text: root (different treatments)


text: Technische Universitat Munchen

text: live

text: Generic

text: northern Prealps, provenance 81024

text: Young seedlings (3 month) were grown in sterile water. 10 seedlings were infected with Phytophthora citricola and 10 were control plants with mock infection. The roots of the two groups were harvested after 6 days (infected plants showing symptoms) and each group's roots were pooled. Subtractive library: Driver = RNA from control roots 98.6% plus 1.4% P. citricola RNA (liquid culture); Tester = RNA from infected roots.

text: E. coli DH10B

characteristic: lab-host

text: Fagus sylvatica

text: SmaI

text: EcoRI

text: hermaphrodite

characteristic: sex

text: root

text: SSH library of pathogen infected Fagus sylvatica roots 1

text: plasmid

text: pBluescript II SK+


characteristic: External Id

text: Technische Universitat Munchen

text: live

text: Generic

text: northern Prealps, provenance 81024

text: Young seedlings (3 month) were grown in sterile water. 10 seedlings were infected with Phytophthora citricola and 10 were control plants with mock infection. The roots of the two groups were harvested after 6 days (infected plants showing symptoms) and each group's roots were pooled. Subtractive library: Driver = RNA from control roots 97% plus P. citricola RNA (liquid culture) 3%; Tester = RNA from infected roots.

text: E. coli NovaBlue

text: Fagus sylvatica

text: EcoRI

text: NotI

text: hermaphrodite

text: root

text: SSH library of pathogen infected Fagus sylvatica roots 2

text: plasmid

text: pBluescript II SK+


text: USDA-ARS

characteristic: INSDC center name

text: live

text: Generic

text: Sarcophaga crassipalpis

text: suppressive subtractive hybridization screening for diapause regulated genes in the flesh fly Sarcophaga crassipalpis


text: National Centre for Cell Science

characteristic: INSDC first public

text: live

text: Generic

text: A cDNA library was prepared using Creator SMART cDNA Library Construction Kit (BD biosciences Clontech, now Clontech Laboratories, Inc. A Takara Bio Company) according to the manufacturer's instructions.

text: Embryonic 16-24h post-fertilization

text: Anopheles stephensi

text: Sfi I

text: Sfi I

text: Whole embryo

text: Embryonic 16-24h post-fertilization library

text: Plasmid

text: pDNR-Lib


characteristic: INSDC last update

text: live

text: Generic

text: Spleen

text: Dicentrarchus labrax

text: Dicentrarchus labrax spleen infected with Vibrio Anguillarum

text: pDNR-lib vector


text: live

text: Generic

characteristic: NCBI submission model

text: Intestine

text: Dicentrarchus labrax

text: Dicentrarchus labrax intestine infected with Vibrio Anguillarum

text: pDNR-lib vector


text: live

text: Generic

characteristic: NCBI submission package

text: Gill

text: Dicentrarchus labrax

text: Dicentrarchus labrax gill infected with Vibrio Anguillarum

text: pDNR-lib vector


text: live

text: Generic

text: Liver

text: Dicentrarchus labrax

characteristic: organism

text: Dicentrarchus labrax liver infected with Vibrio Anguillarum

text: pDNR-lib vector


text: Institute of Marine Biology and Genetics, Hellenic Center of Marine Research

text: live

text: Generic

text: Peritoneal exudate

text: Dicentrarchus labrax

text: Dicentrarchus labrax peritoneal exudate infected with Vibrio Anguillarum

text: pDNR-lib vector


text: live

text: Generic

text: cDNA library was constructed with the Creator SMART library construction kit (BD Bioscience-Clontech, Mountain View, Canada) using the LD PCR based method. Following the manufactures' instructions total RNA was used and between 20 and 22 PCR cycles were performed before size separation of inserts.

text: Kidney

text: Dicentrarchus labrax

text: Dicentrarchus labrax head kidney infected with Vibrio Anguillarum

text: pDNR-lib vector


text: Institute of Crop Sciences, Chinese Academy of Agricultural Sciences (CAAS)

text: live

text: Generic

text: Aegilops speltoides

text: seedling

characteristic: tissue-type

text: Full-length cDNA library of seedling in Ae.speltoides


text: live

text: Generic

text: Chinese spring

text: Triticum aestivum

text: Anther

text: Wheat anther full-length cDNA library

characteristic: title


text: live

text: Generic

text: Chinese spring

text: 0-5 days post anthesis

text: Triticum aestivum

text: endosperm

text: Wheat anther 0-5 days post anthesis full-length cDNA library


text: live

text: Generic

text: Yanzhan 1

text: Triticum aestivum

text: seedling

text: Wheat seedling full-length cDNA library


text: live

text: Generic

text: Yanzhan 1

text: Triticum aestivum

text: shoot

text: Wheat shoot full-length cDNA library


text: live

text: Generic

text: Triticum carthlicum

text: callus

text: Full-length cDNA library of callus in T.carthlicum


text: live

text: Generic

text: Aegilops tauschii

text: Seedling

text: Full-length cDNA library of seedling in Ae.tauschii


text: live

text: Generic

text: Triticum urartu

text: seedling

text: Full-length cDNA library of seedling in T.urartu


text: Dept. Biology and C.R.I.B.I.-Biotechnology Centre, University of Padova

text: live

text: Generic

text: Equal amount (333 ng) of cDNA from DiG01, GDG01, Hae01, Hae02, Hae03, Hae04, Gll01, DiG02, MxT04 have been pooled. Total cDNA was denatured and then incubated at 68oC for 5 h. The double strand cDNA was digested with crab DSN (Evrogen) and the remaining single strand cDNA was PCR amplified, agarose gel size selected (500-2000 bp) and cloned with Gateway Cloning technology (Invitrogen) in pDONR221 (Invitrogen) (Trieste, Italy. April 2006).

text: adult

text: E. coli

text: Mixed tissue

text: Mytilus galloprovincialis

text: pDONR221 (Invitrogen)

characteristic: vector


text: National Research Council of Canada

text: live

text: Generic

text: Windsor Broad

characteristic: cultivar

text: 2 week old embryo

text: Vicia faba

text: Vicia faba cv. Windsor Broad, 2 week old embryo


text: Agriculture and Agri-Food Canada

text: live

text: Generic

text: A directionally cloned (EcoR V/Not I) cDNA library in pcDNA3.1 vector was constructed by Virotech International, Inc. (Gaithersburg, MD) using E. coli DH10B as the host cell.

text: Fully opened flowers

characteristic: dev-stage

text: Adonis aestivalis

text: EcoRV

text: NotI

text: Flower

text: Adonis aestivalis flower


text: University of Houston, Clear Lake

text: live

text: Generic

text: Erythropodium caribaeorum subtractive hybridization cDNA library

characteristic: description

text: Erythropodium caribaeorum

text: Erythropodium caribaeorum cDNA collected Apr-2002, Dania Beach, Florida, USA

text: Plasmid


text: National Institute for Plant Genome Research

text: live

text: Generic

text: PUSA BGD72

text: Subtractive cDNA library made between two drought stressed chickpea (Cicer arietinum) cultivars (BGD72 and ICCV2).

text: Seedling

text: Cicer arietinum

text: Subtracted cDNA library of drought-stressed chickpea cultivar


text: Beijing Institute of Genomics, Chinese Academy of Sciences

text: live

text: Generic

text: Total RNA was extracted from tarantula leg muscle samples and checked for quality. The mRNA was isolated and converted to cDNA. cDNAs larger than 500bp were unidirectionally cloned into the pBluescript II XR Predigested Vector (Stratagene). The titre of the library was 4.0x105 pfu/ml. Clones were picked randomly and inserts end-sequenced using the ABI3730 sequencer.

text: adult

text: leg

characteristic: organ

text: Aphonopelma sp.

text: EcoRI

text: XhoI

text: both male and female

text: skeletal muscle

text: Tarantula skeletal muscle library

text: pBluescriptII


text: Chang Gung University

text: live

characteristic: INSDC status

text: Generic

text: Total RNA was extracted from mid-logarithmic phase P. hominis trophozoites by using TRI REAGENT (Molecular Research Center, Inc.) following the manufacturers' protocols. The integrity and quality of the extracted total RNA was assessed by agarose gel electrophoresis and spectrophormetry. PolyA+ mRNA was isolated from 1 mg of total RNA using polyATtract mRNA isolation system IV (Promega, Madison, USA). Approximately 0.5 ugg of polyA+ mRNA was used for the construction of a directional cDNA library construction by using a cDNA Synthesis Kit (Stratagene, Cedar Creek, USA) following the manufacturer's protocol. The cDNA was size fractionated using by agarose gel electrophoresis followed by purification using Qiaex II Gel extraction kit (Qiagen). Purified cDNA was ligated into EcoRI/XhoI digested pBluescript II XR (Stratagene) using temperature-cycle ligation followed by PCR validation of the ligation reaction. The ligation product was precipitated and electro-transformated into E. coli XL1-Blue MRF' (Stratagene) and plated on LB agar containing IPTG and X-Gal for blue/white selection. Positive clones containing an insert were picked into 2xTY (100 ugg/ml Amp, 10% [vol/vol] glycerol) in 96-well plates and incubated for 24 hours at -37oC for plasmid purification. The insert length of each library was evaluated in 96 clones by PCR. T3 primer was used from the polylinker of the vectors to sequence the 5' end of each insert using standard protocols.

text: Mid-log phase trophozoite

text: Pentatrichomonas hominis

text: XhoI

text: EcoRI

characteristic: strain

text: Ph30000 cDNA Library

text: Plasmid

text: XL1-Blue MRF'


text: ENH Research Institute, Feinberg School of Medicine, Northwestern University

text: live

text: Generic

characteristic: cell type

text: The 3' EST was generated by GLGI reaction that used a CD34 SAGE tags as the sense primer, 3' universal tails as the antisense primer, and CD34 3' cDNAs as the templates for PCR. The amplified fragments were cloned and sequenced.

text: Homo sapiens

text: Human CD34+ ESTs from primary hematopoietic stem-progenitor cells


characteristic: External Id

text: Institute of Oceanology, Chinese Academy of Sciences

text: live

text: Generic

text: The library was constructed from the haemocytes of a Chinese mitten crab challenged by a mixture of Vibrio anguillarum and Staphylococcus aureus

text: E. coli DH10B

characteristic: lab-host

text: Eriocheir sinensis

text: EcoRI

text: XhoI

text: male

characteristic: sex

text: hemoctyes

text: Chinese mitten crab Eriocheir sinensis hemocytes cDNA Library

text: Cosmid

text: pBlueScript II SK(+)


text: National Center of Excellence in Molecular Biology

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: cDNA library was constructed from mRNA of drought stressed leaves. Clones were randomly picked and sequenced.

text: 15 days of stress

text: LEAF

text: Gossypium arboreum

text: BsrG I

text: BsrG I

text: hermaphrodite

text: Total leaf tissue

text: cDNA library from leaves of Gossypium arboreum

text: Plasmid


text: East China Normal University

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Macrobrachium nipponense

characteristic: organism

text: ovary

text: Oriental river prawn ovary cDNA library

text: Plasmid

text: pBluescriptII SK+


text: Instituto Butantan

text: live

text: Generic

text: venom glands

text: Micrurus corallinus

text: EcoRI

text: NotI

text: venom glands

characteristic: tissue-type

text: Coral snake Micrurus corallinus cDNA library

characteristic: title

text: Phage

text: Lambda GT11

characteristic: vector


text: Gembloux Agricultural University

text: live

text: Generic

characteristic: cultivar

text: Genes(mRNA) which their expression was misregulated by pathogen infection.

characteristic: description

text: Malus pumila

text: Hermaphrodite

text: Leaves

text: Apple cDNA library related to pathogen infection


text: Institute of Biology - Amazonas Federal University

text: live

characteristic: INSDC status

text: Generic

text: sub-adult male

characteristic: dev-stage

text: Bothrops atrox

text: male

text: Viper venom gland cDNA library

text: pDNR


text: Rubber Research Institute of India

text: live

text: Generic

text: PR 261

text: Total RNA was prepared from leaf tissues of rubber tree subjected to cold stress and also from the normal plant belonging to the same clones. Messenger RNA was purified from total RNA using PolyATract mRNA Isolation System (Promega). cDNA synthesis followed by suppression subtractive hybridization (SSH) was carried out using the PCR Select cDNA Subtraction Kit (Clontech). PCR-amplified products resulting from two rounds of hybridization were cloned randomly into the vector pTZ57R/T (MBI Fermentas). Colony PCR using vector directed primers was used to identify recombinant plasmids containing inserts. Recombinant plasmids were subjected to single-pass sequencing reactions using M13 forward primer.

text: Hevea brasiliensis

text: Mature leaf

text: GA_HbSCDNA Library

text: Plasmid

text: pTZ57R/T (MBI Fermentas)


text: Soochow University

text: live

text: Generic

text: We randomly choose five adult shrimps and mix them in one sample. We use the suppression subtractive hybridization to obtain the subtracted cDNA library of differentially expressed genes in Neocaridina denticulata that exposed to the endocrine disrupter dipropyl phthalate (DPrP). We use the the pGEM-T vector and the competent cell Escherischia coli JM109 to construct the cDNA library.

text: Escherischia coli JM109

text: Neocaridina denticulata

text: Cephalothorax

text: Differentially Expressed Genes in Neocaridina denticulata Exposed to the Endocrine Disrupter Dipropyl Phthalate (DPrP)

text: Plasmid

text: pGEM-T vector


text: Dairy and Swine Research and Development Center, Agriculture and Agri-Food Canada.

text: live

text: Generic

text: Sus scrofa

text: female

text: Synthetic Genex 3000 and Genex Meishan

characteristic: strain

text: semitendinosus muscle

text: Sus scrofa semitendinosus muscle cDNA subtracted library

text: pT-Adv vector


characteristic: External Id

text: Dept. Biology and C.R.I.B.I.-Biotechnology Centre, University of Padova

text: live

text: Generic

text: RNA was extracted after 3 days of treatment with okadaic acid (Trieste, Spring 2005). The cDNA was synthesized with a modified SMART approach (Clontech). The oligos were designed with attB-sequences allowing size-selected cDNA cloning with Gateway Cloning technology (Invitrogen) in pDONR221 (Invitrogen) (Trieste, Italy. January 2006).

text: adult

text: E. coli

text: Digestive gland

characteristic: organ

text: Mytilus galloprovincialis

text: pDONR221 (Invitrogen)


text: live

text: Generic

text: RNA was isolated from mussel digestive glands after treatment with inactivated cocktail of Vibrio sp. (Padua, Italy). The cDNA was synthesized from mRNA with a modified SMART approach (Clontech). The oligos were designed with attB-seque nces allowing size-selected cDNA cloning with Gateway Cloning technology (Invitrogen) in pDONR221 (Invitrogen) (Trieste, Italy. January 2006).

text: adult

text: E. coli

characteristic: lab-host

text: Digestive gland

text: Mytilus galloprovincialis

text: pDONR221 (Invitrogen)


characteristic: INSDC center name

text: live

text: Generic

text: RNA was extracted from digestive gland, gills, foot, gonads, haemolymph and mantle. Cloned in Padova, Italy. February 2001

text: adult

text: E. coli

text: Mixed tissue

text: Mytilus galloprovincialis

text: pDONR221 (Invitrogen)


characteristic: INSDC first public

text: live

text: Generic

text: RNA was extracted after treatment with 2 cocktails of organic compounds and heavy metals (cfr Venier et al.). The cDNA was synthesized from mRNA with a modified SMART approach (Clontech). The oligos were designed with attB-sequences allowing size-selected cDNA cloning with Gateway Cloning technology (Invitrogen) in pDONR221 (Invitrogen) (Trieste, Italy. January 2006).

text: adult

text: E. coli

text: digestive gland and gills

text: Mytilus galloprovincialis

text: pDONR221 (Invitrogen)


characteristic: INSDC last update

text: live

text: Generic

text: NA was isolated from mussel gills after treatment with inactivated cocktail of Vibrio sp. (Padua, Italy). The cDNA was synthesized from mRNA with a modified SMART approach (Clontech). The oligos were designed with attB-sequences allowing size-selected cDNA cloning with Gateway Cloning technology (Invitrogen) in pDONR221 (Invitrogen) (Trieste, Italy. January 2006).

text: adult

text: E. coli

text: Gills

text: Mytilus galloprovincialis

text: pDONR221 (Invitrogen)


text: live

text: Generic

characteristic: NCBI submission model

text: RNA was isolated from haemocytes of control mussels by P. Venier in Padova. The cDNA was synthesized from mRNA with a modified SMART approach (Clontech). The oligos were designed with attB-sequences allowing size-selected cDNA cloning with Gateway Cloning technology (Invitrogen) in pDONR221 (Invitrogen) (Trieste, Italy. January 2006).

text: adult

text: E. coli

text: Haemolymph

text: Mytilus galloprovincialis

text: pDONR221 (Invitrogen)


text: live

text: Generic

characteristic: NCBI submission package

text: RNA was isolated from mussel haemocytes after treatment with inactivated cocktail fo Vibrio sp. (Padova, Italy). The cDNA was synthesized from mRNA with a modified SMART approach (Clontech). The oligos were designed with attB-sequences allowing size-selected cDNA cloning with Gateway Cloning technology (Invitrogen) in pDONR221 (Invitrogen) (Trieste, Italy. January 2006).

text: adult

text: E. coli

text: Haemolymph

text: Mytilus galloprovincialis

text: pDONR221 (Invitrogen)


text: live

text: Generic

text: Control library (January 2006 - Vigo, Spain).

text: adult

text: E. coli

text: Haemolymph

text: Mytilus galloprovincialis

characteristic: organism

text: pDONR221 (Invitrogen)


text: live

text: Generic

text: Gills, digestive gland, foot, anterior and posterior adductor muscles and mantle (November 2000 - Padova, Italy).

text: adult

text: E. coli

text: Mixed tissue

text: Mytilus galloprovincialis

text: pDONR221 (Invitrogen)


text: College of Food Science & Nutrition Engineering, China Agricultural University

text: live

text: Generic

text: Growing stage

text: E.coli DH5alpha

text: Vitis amurensis

text: EcoRI

text: young leaves inoculated with anthracnose pathogen (Sphaceloma ampelinum)

characteristic: tissue-type

text: suppressive subtraction cDNA library of Chinese wild Vitis amurensis

text: Plasmid

text: T-Easy Vector


text: live

text: Generic

text: Growing stage

text: E.coli DH5alpha

text: Vitis quinquangularis

text: EcoRI

text: Guangxi mao putao

text: suppressive subtraction cDNA library of Chinese wild Vitis quinquangularis

characteristic: title

text: Plasmid

text: T-Easy Vector


text: Central Salt and Marine Chemicals Research Institute (CSIR)

text: live

text: Generic

text: 45 days

text: Salicornia brachiata

text: XhoI

text: shoot tissue

text: Salicornia brachiata 45 days shoot cDNA library

text: Phage

text: Lambda Zap

characteristic: vector


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: growth in rich medium ( 2% malt extract, 2% glucose, 0.5% peptone) for 5 days on rotary shaker at room temp. The library was made from oligo(dT)-primed cDNA and cloned into the p21_cDNA vector. PolyA RNA was primed with an oligo(dT) primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'-CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 0.5-1.2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested p21_cDNA vector. Library was created and sequencing done by the DOE Joint Genome Institute (Walnut Creek, CA).

text: Postia placenta

text: SalI

text: NotI

text: MAD 698

text: CAIF Postia placenta in rich medium


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: growth in rich medium ( 2% malt extract, 2% glucose, 0.5% peptone) for 5 days on rotary shaker at room temp. The library was made from oligo(dT)-primed cDNA and cloned into the pMCL200_cDNA vector. PolyA RNA was primed with oligo(dT) primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'-CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 0.5-1.2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pMCL200_cDNA vector. Library was created and sequencing done by the DOE Joint Genome Institute (Walnut Creek, CA).

text: Postia placenta

text: SalI

text: NotI

text: MAD 698

text: CAIG Postia placenta in rich medium


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: growth in rich medium ( 2% malt extract, 2% glucose, 0.5% peptone) for 5 days on rotary shaker at room temp. The library was made from oligo(dT)-primed cDNA and cloned into the p21_cDNA vector. PolyA RNA was primed with an oligo(dT) primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'-CGGACGCGTGGG) and digested with NotI. cDNA was size selected >2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested p21_cDNA vector. Library was created and sequencing done by the DOE Joint Genome Institute (Walnut Creek, CA).

text: Postia placenta

text: SalI

text: NotI

text: MAD 698

text: CAIH Postia placenta in rich medium


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: growth in rich medium ( 2% malt extract, 2% glucose, 0.5% peptone) for 5 days on rotary shaker at room temp. The library was made from oligo(dT)-primed cDNA and cloned into the pMCL200_cDNA vector. PolyA RNA was primed with oligo(dT) primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'-CGGACGCGTGGG) and digested with NotI. cDNA was size selected >2.5kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pMCL200_cDNA vector. Library was created and sequencing done by the DOE Joint Genome Institute (Walnut Creek, CA).

text: Postia placenta

text: SalI

text: NotI

text: MAD 698

text: CAII Postia placenta in rich medium


text: DOE Joint Genome Institute

text: live

text: Generic

text: Postia placenta

text: SalI

text: NotI

text: MAD 698

text: CAIN Postia placenta in rich medium


text: DOE Joint Genome Institute

text: live

text: Generic

text: Postia placenta

text: SalI

text: NotI

text: MAD 698

text: CAIO Postia placenta in rich medium


text: DOE Joint Genome Institute

text: live

text: Generic

text: Postia placenta

text: SalI

text: NotI

text: MAD 698

text: CAIP Postia placenta in rich medium


text: DOE Joint Genome Institute

text: live

text: Generic

text: Postia placenta

text: SalI

text: NotI

text: MAD 698

text: CAIS Postia placenta in rich medium


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: Grown on minimal salts plus 0.5% Glucose as sole carbon source for 5 days on rotary shaker at room temp. The library was made from oligo(dT)-primed cDNA and cloned into the pMCL200_cDNA vector. PolyA RNA was primed with oligo(dT) primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'-CGGACGCGTGGG) and digested with NotI. cDNA was size selected >2.5kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pMCL200_cDNA vector. Library was created and sequencing done by the DOE Joint Genome Institute (Walnut Creek, CA).

text: Postia placenta

text: SalI

text: NotI

text: MAD 698

text: CATO Postia placenta in Glucose medium


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: Grown on minimal salts plus 0.5% Glucose as sole carbon source for 5 days on rotary shaker at room temp. The library was made from oligo(dT)-primed cDNA and cloned into the pMCL200_cDNA vector. PolyA RNA was primed with oligo(dT) primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'-CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 0.5-1.2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pMCL200_cDNA vector. Library was created and sequencing done by the DOE Joint Genome Institute (Walnut Creek, CA).

text: Postia placenta

text: SalI

text: NotI

text: MAD 698

text: CATP Postia placenta in Glucose medium


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: Grown on minimal salts plus 0.5% microcrystalline cellulose (=Avicel) as sole carbon source for 5 days on rotary shaker at room temp. The library was made from oligo(dT)-primed cDNA and cloned into the pMCL200_cDNA vector. PolyA RNA was primed with oligo(dT) primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'-CGGACGCGTGGG) and digested with NotI. cDNA was size selected >2.5kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pMCL200_cDNA vector. Library was created and sequencing done by the DOE Joint Genome Institute (Walnut Creek, CA).

text: Postia placenta

text: SalI

text: NotI

text: MAD 698

text: CATS Postia placenta in Avicel medium


text: DOE Joint Genome Institute

text: live

text: Generic

text: In Vivo Conditions: Grown on minimal salts plus 0.5% microcrystalline cellulose (=Avicel) as sole carbon source for 5 days on rotary shaker at room temp. The library was made from oligo(dT)-primed cDNA and cloned into the pMCL200_cDNA vector. PolyA RNA was primed with oligo(dT) primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'-CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 0.5-1.2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pMCL200_cDNA vector. Library was created and sequencing done by the DOE Joint Genome Institute (Walnut Creek, CA).

text: Postia placenta

text: SalI

text: NotI

text: MAD 698

text: CATT Postia placenta in Avicel medium


text: Dept. Biology and C.R.I.B.I.-Biotechnology Centre, University of Padova

text: live

text: Generic

text: mRNA was isolated from mussel RNA extracted after treatment with inactivated cocktail of Vibrio sp. (Padua, Italy and Vigo, Spain) The cDNA was synthesized from mRNA with a modified SMART approach (Clontech). The oligos were designed with attB-sequences allowing size-selected cDNA cloning with Gateway Cloning technology (Invitrogen) in pDONR221 (Trieste, Italy. January 2006)

text: adult

text: E. coli

text: Mixed tissue

text: Mytilus galloprovincialis

text: pDONR221 (Invitrogen)


text: University of Florida

text: live

text: Generic

text: Approximately 2000 worker termite guts were dissected and cleared of symbionts. The dissected tissue was then used for RNA extraction.

text: Reticulitermes flavipes

text: Whole gut

text: Termite gut library


text: Marine Biology

text: live

text: Generic

text: photosynthetic cells

characteristic: cell type

text: sporophyte

text: Ulva fasciata

text: thallus

text: Ulva fasciata 24-h hypersalinity treatment Library


text: Soochow University

text: live

text: Generic

text: We identified differentially expression genes of Neocaridina denticulata exposed to NP by suppression subtractive hybridization (SSH). We use the the pGEM-T vector and the competent cell Escherischia coli JM109 to construct the cDNA library.

characteristic: description

text: Escherichia coli JM109

text: Neocaridina denticulata

text: Subtracted cDNA Library of Differentially Expressed Genes in Neocaridina denticulata Exposed to the Nonylphenol

text: Plasmid

text: pGEM-T vector


text: ICRISAT

text: live

characteristic: INSDC status

text: Generic

text: JL 24: rd29A:DREB1A transgenic

characteristic: cultivar

text: Partial sequence obtained by DDRT-PCR from drought induced leaves. Peanut plants were grown in pots contained soil, sand and farm yard manure (3:2:1) mixture and maintained at 100% field capacity by watering daily, in the P2 level green house. Thirty day-old plants were subjected to moisture stress by withholding water. Newly expanded leaves were collected from non-transgenic JL 24 and transgenic JL 24:rd29A:DREB1A stressed plants and placed into liquid N2 immediately and stored in -80oC freezer. Total RNA was isolated with TRIzol Reagent (Invitrogen life technologies, CA 92008, USA) from the leaf samples. Differential display of cDNA was performed using RNAimage (GenHunter Corporation, TN 37211, USA) kit following the manufacturer's protocol. Differentially expressed transcripts were isolated from the gel. Re-amplified PCR fragments were cloned in to pGem-T Easy Vector (Promega Madison, WI 53711-5399 USA) and sequenced using M13 primers. Sequences are trimmed to remove vector and cloning oligo sequences contaminants.

text: 30 day-old seedlings

text: E.coli DH10B

text: Arachis hypogaea

text: drought-stressed leaves

text: peanut (JL 24:rd29A:DREB1A transgenic) drought stressed differentially expressed cDNA

text: pGEM-T Easy (Promega)


text: ICRISAT

text: live

text: Generic

text: JL 24: rd29A:DREB1A transgenic

text: Partial sequence obtained by DDRT-PCR from salt induced leaves. Peanut plants were grown in MS agar tubes in the tissue culture lab and after two weeks of germination plants were transferred to test tubes containing MS liquid medium and plants were grown for one week in liquid medium. Three weeks old plants were subjected to salt stress treatments by transferring in to a fresh tube containing MS liquid plus 200 mM NaCl. Leaf tissue samples were collected from non-transgenic JL 24 and transgenic JL 24:rd29A:DREB1A stressed plants and placed into liquid N2 immediately and stored in -80oC freezer. Total RNA was isolated with TRIzol Reagent (Invitrogen life technologies, CA 92008, USA) from the leaf samples. Differential display of cDNA was performed using RNAimage (GenHunter Corporation, TN 37211, USA) kit following the manufacturer's protocol. Differentially expressed transcripts were isolated from the gel. Re-amplified PCR fragments were cloned in to pGem-T Easy Vector (Promega Madison, WI 53711-5399 USA) and sequenced using M13 primers. Sequences are trimmed to remove vector and cloning oligo sequences contaminants.

text: 21 day-old seedlings

characteristic: dev-stage

text: E.coli DH10B

text: Arachis hypogaea

text: salt-stressed leaves

text: peanut (JL 24:rd29A:DREB1A transgenic) salt stressed differentially expressed cDNA

text: pGEM-T Easy (Promega)


characteristic: External Id

text: University of Florida

text: live

text: Generic

text: specific_host: Reticulitermes flavipes; The digestive tracts from 300 mature worker termites were dissected. Released hindgut luminal contents were pooled and subjected to centrifugation to collect symbiotic protist cells. The cell pellets were used for RNA extraction.

text: termite gut metagenome

text: Reticulitermes flavipes symbiont library


text: Northwest A&F University

text: live

text: Generic

text: adult plant and seedling

text: Triticum aestivum

text: wheat leaves

text: Transcript derived fragments (TDFs) from wheat leaves challenged with stripe rust fungus (CY32)

text: pGEM-T easy


text: Embrapa Genetic Resources & Biotechnology

text: live

text: Generic

text: Pisang Klutug Wulung (PKW) - BB

text: Total RNA was extracted using the RNeasy Plant Mini Kit (QIAGEN - Protocol: Purification of total RNA from plant cells and tissues and filamentous fungi). Then, the mRNA was obtained using the Micro-FastTrack 2.0 Kit (Invitrogen - Protocol: mRNA isolation from total RNA + Basic mRNA isolation method). The mRNA was then used to produce the cDNA library using the Creator SMART cDNA Library Construction Kit (Clontech Laboratories - Protocol: SMART cDNA synthesis by LD PCR + Ligation of ds cDNA to pDNR-LIB). The PrimeScript Reverse transcriptase (TAKARA BIO INC) and the Advantage 2 PCR Kit (Clontech Laboratories) were also used in the preparation of this cDNA library.

text: Plants cultivated under hydroponics condition - 30 cm tall

characteristic: isolate

text: Roots

characteristic: organ

text: Musa balbisiana

text: Sfi IA

text: Sfi IB

text: Hermaphrodite

characteristic: sex

text: Embrapa_Musa balbisiana_PKW_Root

text: Plasmid

text: pDNR-LIB


characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: Pisang Klutug Wulung (PKW) - BB

text: Leaf

text: Musa balbisiana

text: Sfi IA

text: Sfi IB

text: Hermaphrodite

text: Embrapa_Musa balbisiana_PKW_Leaf

text: Plasmid

text: pDNR-LIB


text: Soochow University

characteristic: INSDC last update

text: live

text: Generic

characteristic: NCBI submission model

text: hemocytes

text: healthy and unmoulted giant freshwater prawns

text: Macrobrachium rosenbergii

text: hemolymph

text: LPS-treated 6 hr Library


text: live

text: Generic

characteristic: NCBI submission package

text: Cachaco

text: The dehydration-tolerant banana variety Cachaco (ABB cooking banana). Total RNA was extracted using the RNeasy Plant Mini Kit (QIAGEN - Protocol: Purification of total RNA from plant cells and tissues and filamentous fungi). Then, the mRNA was obtained using the Micro-FastTrack 2.0 Kit (Invitrogen - Protocol: mRNA isolation from total RNA + Basic mRNA isolation method). The mRNA was then used to produce the cDNA library using the Creator SMART cDNA Library Construction Kit (Clontech Laboratories - Protocol: SMART cDNA synthesis by LD PCR + Ligation of ds cDNA to pDNR-LIB). The PrimeScript Reverse transcriptase (TAKARA BIO INC) and the Advantage 2 PCR Kit (Clontech Laboratories) were also used in the preparation of this cDNA library.

text: Cauliflower-like shoot meristems submitted to 2-week acclimation on 0.4M sucrose

text: Cauliflower-like shoot meristems

text: Musa ABB Group

characteristic: organism

text: Sfi IA

text: Sfi IB

text: Hermaphrodite

text: Plasmid

text: pDNR-LIB


text: live

text: Generic

text: Cachaco

text: Cauliflower-like shoot meristems not submitted to 2-week acclimation on 0.4M sucrose

text: Cauliflower-like shoot meristems

text: Musa ABB Group

text: Sfi IA

text: Sfi IB

text: Hermaphrodite

characteristic: title

text: Plasmid

text: pDNR-LIB


text: Lund University

text: live

text: Generic

text: Protopterus aethiopicus

text: liver

characteristic: tissue-type

text: Lungfish liver library


text: Austrian Research Centers GmbH - ARC

text: live

text: Generic

text: Yungay

text: Potato cv. Yungay (Peru) cDNA library targeting genes expressed in leaves during interaction with the potato pathogen Phytophthora infestans (a virulent isolate). Adult plants prior to blooming were spray-inoculated, leaves were harvested and immersed immediately in liquid nitrogen, at 4 dpi when first disease symptoms were visible. This library is part of the RLP microarray at the Trognitz lab. Normalization was achieved by one cycle of melting and re-association of the cDNA, followed by separation of the remaining ss-cDNA (normalized cDNA) from the re-associated ds-cDNA by passing through hydroxylapatite column chromatography. The ss-cDNA was amplified in 11 LA-PCR cycles. For directional cloning, cDNA was subjected to partial exonuclease treatment to generate 5'-Eco RI overhangs and then digested with Not I. Size fractionation of Not I treated cDNAs >0.5 kb was done on agarose gels. The cDNA was directionally ligated into vector. Ligations were electroporated into T1 phage-resistant TransforMaxTM EC100TM-T1 (Epicentre) electro-competent cells.

text: adult

text: Leaf

text: Solanum tuberosum subsp. andigenum

text: Eco RI

text: Not I

text: Solanum tuberosum cv. Yungay adult leaf, normalized

text: Plasmid

text: pBS II sk+

characteristic: vector


text: FUNDP, Namur

text: live

text: Generic

text: Arancha

text: 21 weeks old (09/03/02)

text: Leaf

text: Cichorium intybus

text: CiFC

text: plasmid

text: pBluescript II ks+


text: FUNDP, Namur

text: live

text: Generic

text: Arancha

text: Nodule

text: Cichorium intybus

text: CiNO

text: plasmid

text: pBluescript II ks+


text: FUNDP, Namur

text: live

text: Generic

text: Arancha

text: 20 weeks old (08/27/02)

text: Root

text: Cichorium intybus

text: CiRB

text: plasmid

text: pBluescript II ks+


text: FUNDP, Namur

text: live

text: Generic

text: Arancha

text: 21 weeks old (09/03/02)

text: Root

text: Cichorium intybus

text: CiRC

text: plasmid

text: pBluescript II ks+


text: FUNDP, Namur

text: live

text: Generic

text: Arancha

text: 24 weeks old (09/24/02)

text: Root

text: Cichorium intybus

text: CiRE

text: plasmid

text: pBluescript II ks+


text: FUNDP, Namur

text: live

text: Generic

text: Arancha

text: 26 weeks old (10/07/02)

text: Root

text: Cichorium intybus

text: CiRG

text: plasmid

text: pBluescript II ks+


text: FUNDP, Namur

text: live

text: Generic

text: Arancha

text: 28 weeks old (10/22/02)

text: Root

text: Cichorium intybus

text: CiRK

text: plasmid

text: pBluescript II ks+


text: FUNDP, Namur

text: live

text: Generic

text: Arancha

text: 30 weeks old (11/05/02)

text: Root

text: Cichorium intybus

text: CiRL

text: plasmid

text: pBluescript II ks+


text: FUNDP, Namur

text: live

text: Generic

text: Arancha

text: 32 weeks old (11/21/02)

text: Root

text: Cichorium intybus

text: CiRN

text: plasmid

text: pBluescript II ks+


text: FUNDP, Namur

text: live

text: Generic

text: Arancha

text: 33 weeks old (11/29/02)

text: Root

text: Cichorium intybus

text: CiRO

text: plasmid

text: pBluescript II ks+


text: FUNDP, Namur

text: live

text: Generic

text: Arancha

text: 35 weeks old (12/13/02)

text: Root

text: Cichorium intybus

text: CiRQ

text: plasmid

text: pBluescript II ks+


text: FUNDP, Namur

text: live

text: Generic

text: Arancha

text: 40 weeks old (01/17/03)

text: Root

text: Cichorium intybus

text: CiRS

text: plasmid

text: pBluescript II ks+


text: Centre for Environmental Stress and Adaptation Research, Bio21 Institute, Genetics Department, University of Melbourne

text: live

text: Generic

text: Total RNA was extracted using Trizol (Invitrogen) and messenger RNA was isolated using Nucleotrap mRNA kit (Macherey-Nagel). First strand cDNA was prepared using the Creator SMART cDNA Library Construction Kit (Clontech) with two modifications: a modified 3' primer (5'-AAGCAGTGGTATCAACGCAGAGTGGCCGAGGCGGCCTGTTTTGTTTTTTTTTCTTTTTTTTTTVN-3') and a 1:1 mixture of ArrayScript Reverse Transcriptase (Ambion) and BioScript Reverser transcriptase (Bioline). Second strand cDNA was synthesised using the Advantage 2 PCR System (Clontech) with adapter-specific primer (5'-AAGCAGTGGTATCAACGCAGAGT-3'). Double stranded cDNA was purified using the DNA Clean And Concentrator (Zymogen) and digested with Sfi-I restriction enzyme (New England Biolabs). Digested cDNA was purified and ligated to pDNR-Lib vector (Clontec) and heat transformed into JM109 (Promega). 576 random colonies were picked and the cDNA inserts were amplified using M13 primers. Unincorporated nucleotides and oligos were removed using ExoZap (Stratagene) and the amplicons were sequenced from the M13F end.

characteristic: description

text: adult

characteristic: lab-host

text: Micropterix calthella

text: Sfi-I A

text: Sfi-I B

text: female and male

text: whole body

text: Micropterix calthella adult whole body cDNA library

text: plasmid

text: pDNR-Lib


text: University of Padova

text: live

characteristic: INSDC status

text: Generic

text: Leccino

characteristic: cultivar

text: All samples collected at post-fruit set to veraison developmental stages. Suppression Subtractive Hybridization (SSH) performed by using Clontech PCR-Select cDNA Subtraction Products.

text: Olea europaea

text: fruitlet

text: Olea europaea cv. Leccino fruitlet


characteristic: External Id

text: Embrapa Cenargen

text: live

text: Generic

text: IAC 96/414

text: Total RNA was extracted from frozen root tissues using CONCERT Large-Scale RNA Isolation (Invitrogen) according to the manufacture's directions. Poly-A+ mRNA was isolated using an Oligotex mRNA Bath Protocol kit according to manufacturer's manual (QIAGEN). A cDNA library was constructed from four micrograms of poly-A+ mRNA, measured by absorbance at 260 nm, in the pSPORT 1 vector exploiting the NotI and SalI restrictions sites at the 5' and 3' ends respectively. It was used Superscript Plasmid System with Gateway Technology for cDNA Synthesis and Cloning (Invitrogen) according manufacturer's directions.

text: Roots

text: Gossypium hirsutum

text: NotI

text: SalI

text: Root

text: Cotton resistant to Meloidogyne incognita

text: Plasmid

text: pSPORT 1


text: Embrapa Cenargen

text: live

text: Generic

text: IAC 98/708

text: Roots

text: Gossypium hirsutum

text: NotI

text: SalI

text: Root

text: Cotton susceptible to Meloidogyne incognita

text: Plasmid

text: pSPORT 1


text: The Johns Hopkins University

text: live

text: Generic

text: Brain cDNA Library, Suppression Subtraction Hybridization PCR, enriched for differentially expressed transcripts resulting from oral exposure to cyclotrimethylenetrinitramine (RDX) and 2,6-dinitrotoluene (2,6-DNT).

text: Colinus virginianus

text: Brain

text: Northern bobwhite quail brain cDNA


text: Soochow University

text: live

text: Generic

text: hemocytes

characteristic: cell type

characteristic: dev-stage

text: Macrobrachium rosenbergii

text: hemolymph

text: ODN-treated 12 hr Library


text: Oklahoma State University

text: live

text: Generic

text: Total RNA was isolated from pooled guts and salivary glands of I. scapularis female ticks injected with subolesin dsRNA or injection buffer at 9 dpi (8 days of feeding) using TriReagent (Sigma) according to manufacturer's instructions. SSH was performed at Evrogen JCS (Moscow, Russia) as described previously (Naranjo et al., 2006; FEMS Immunol Med Microbiol 46: 298-312).

text: Escherichia coli

text: Ixodes scapularis

text: female

characteristic: sex

text: pooled guts and salivary glands

text: Subolesin dsRNA and saline injected Ixodes scapularis SSH Library

text: Plasmid


text: Yokohama National University

characteristic: INSDC center name

text: live

text: Generic

text: HiCEP peak; offspring of a parthenogenetic female (TRPM-AAr-0000630); country: Japan: Yamanashi, Fujiyoshida

text: 10-12 days old

text: Folsomia candida

text: parthenogenetic female

text: Fuji-Japan

characteristic: strain

text: whole body

text: Folsomia candida whole body Fuji-Japan 10-12 days old


text: Yokohama National University

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Folsomia candida

text: parthenogenetic female

text: Fuji-Japan

text: whole body

text: Folsomia candida whole body Fuji-Japan


text: IMBIO, University Bonn

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Craterostigma plantagineum

text: Craterostigma plantagineum mRNA


text: Korea Polar Research Institute (KOPRI)

text: live

text: Generic

text: Clontech's SMART cDNA Library Construction Kit (catalog number 634901) was used to construct the cDNA library. After cDNA synthesis by LD-PCR and size fractionation, the column eluent was then ligated into pTriplEx2 vector that has been digested with SfiI. The ligated DNA was transformed into BM25.8.

text: Aulacomnium turgidum

characteristic: organism

text: SfiI

text: SfiI

text: whole plant

text: Aulacomnium turgidum cDNA Library

characteristic: title


text: Korea Polar Research Institute (KOPRI)

text: live

text: Generic

text: Clontech's Creator SMART cDNA Library Construction Kit (catalog number 634903) was used to construct the cDNA library. After cDNA synthesis by LD-PCR and size fractionation, the column eluent was then ligated into pDNR-LIB vector that has been digested with SfiI. The ligated DNA was transformed into DH5a.

text: Deschampsia antarctica

text: SfiI

text: SfiI

text: Leaf

characteristic: tissue-type

text: Deschampsia antarctica Antarctic Library

text: pDNR-LIB

characteristic: vector


text: Korea Polar Research Institute (KOPRI)

text: live

text: Generic

text: Stratagene's cDNA Synthesis Kit (catalog number 200401), ZAP Express Vector Kit (catalog number 239211), and Gigapack III Gold Packaging Extract (catalog number 200201) were used to construct the cDNA library. After second-strand cDNA synthesis, adapter ligation, and size fractionation, the column eluent was then ligated into Stratagene's UniZAP XR vector that has been digested with EcoRI and XhoI, and phosphorylated by Stratagene. The ligated DNA was packaged using Stratagene's Gigapack III Gold Packaging Extract and the pBluescript SK phagemid and insert was circularized to phagemid by in vivo excision.

characteristic: description

text: Deschampsia antarctica

text: EcoRI

text: XhoI

text: Leaf

text: Deschampsia antarctica Greenhouse Library

text: pBluescript SK


text: University of Leeds

text: live

characteristic: INSDC status

text: Generic

text: embryo

text: Caenorhabditis elegans

text: hermaphrodite

text: Yuji Kohara:Caenorhabditis elegans N2 hermaphrodite embryo


text: University of Leeds

text: live

text: Generic

text: Caenorhabditis elegans

text: hermaphrodite

text: whole Caenorhabditis elegans

text: oligo-capped cDNA library


text: Tsukuba University

text: live

text: Generic

text: Sakurajima Daikon

characteristic: cultivar

text: 4-d old seedling

text: Raphanus sativus var. niger

text: subtraction library radish hypocotyl cDNA


characteristic: External Id

text: National Institute of Allergy and Infectious Diseases

text: live

text: Generic

text: unidirectional library was constructed using long distance PCR (Smart cDNA kit) and Lambda TriplEx2 vector

text: adult female

text: salivary glands

characteristic: organ

text: Anopheles darlingi

text: female

text: Adult female salivary gland cDNA library Adarg3 primer extension and clone resequencing project

text: Phage

text: Lambda TriplEx2


text: British Antarctic Survey

text: live

text: Generic

text: Krill collected in upper 200m of the water column (S65.15',W4.45') by RV Polarstern (ANTXXI-4) on 14 March 2004. Krill then subjected to 12h light, 12h darkness (LD), continous light (LL), or continous darkness (DD) conditions for 1 week prior to sampling.

text: Euphausia superba

text: Head

text: Euphausia superba LD-DD SSH cDNA Library

text: Plasmid

text: pGem-T Easy


text: British Antarctic Survey

text: live

text: Generic

text: Euphausia superba

text: Head

text: Euphausia superba LD cDNA Library

text: Plasmid

text: pGem-T Easy


text: DOE Joint Genome Institute

text: live

text: Generic

text: pool of Kanlow, Summer, Shawnee, Blackwell

text: The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected >2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed and sequencing performed at the DOE Joint Genome Institute (Walnut Creek, CA).

text: DH10B T1-resistant

characteristic: lab-host

text: apex and stem

text: Panicum virgatum

text: SalI

text: NotI

text: CCGA Panicum virgatum apex + stem (H)


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 0.6-2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed and sequencing performed at the DOE Joint Genome Institute (Walnut Creek, CA).

text: DH10B T1-resistant

text: apex and stem

text: Panicum virgatum

text: SalI

text: NotI

text: CCGB Panicum virgatum apex + stem (L)


text: DOE Joint Genome Institute

text: live

text: Generic

text: pool of Kanlow, Summer, Shawnee

text: DH10B T1-resistant

text: early floral buds + reproductive tissue

text: Panicum virgatum

text: SalI

text: NotI

text: CCGC Panicum virgatum early floral buds + reproductive tissue (H)


text: DOE Joint Genome Institute

text: live

text: Generic

text: pool of Kanlow, Summer, Shawnee

text: DH10B T1-resistant

text: early floral buds + reproductive tissue

text: Panicum virgatum

text: SalI

text: NotI

text: CCGF Panicum virgatum early floral buds + reproductive tissue (L)


text: DOE Joint Genome Institute

text: live

text: Generic

text: pool of Kanlow, Summer, Shawnee

text: DH10B T1-resistant

text: late flowering buds + seed development

text: Panicum virgatum

text: SalI

text: NotI

text: CCGG Panicum virgatum late flowering buds + seed development (H)


text: DOE Joint Genome Institute

text: live

text: Generic

text: pool of Kanlow, Summer, Shawnee

text: DH10B T1-resistant

text: late flowering buds + seed development

text: Panicum virgatum

text: SalI

text: NotI

text: CCGH Panicum virgatum late flowering buds + seed development (L)


text: DOE Joint Genome Institute

text: live

text: Generic

text: pool of Kanlow, Summer, Shawnee

text: DH10B T1-resistant

text: etiolated seedlings

text: Panicum virgatum

text: SalI

text: NotI

text: CCGI Panicum virgatum etiolated seedlings (H)


text: DOE Joint Genome Institute

text: live

text: Generic

text: pool of Kanlow, Summer, Shawnee

text: DH10B T1-resistant

text: etiolated seedlings

text: Panicum virgatum

text: SalI

text: NotI

text: CCGN Panicum virgatum etiolated seedlings (L)


text: DOE Joint Genome Institute

text: live

text: Generic

text: pool of Kanlow, Summer, Trailblazer

text: DH10B T1-resistant

text: root

text: Panicum virgatum

text: SalI

text: NotI

text: CCGO Panicum virgatum root (H)


text: DOE Joint Genome Institute

text: live

text: Generic

text: pool of Kanlow, Summer, Trailblazer

text: DH10B T1-resistant

text: root

text: Panicum virgatum

text: SalI

text: NotI

text: CCGP Panicum virgatum root (L)


text: DOE Joint Genome Institute

text: live

text: Generic

text: Alamo A4 single genotype

text: The library was made from oligo(dT)-primed cDNA and cloned into vector pDNR-LIB (Clontech Laboratories, Inc., Mountain View, CA) using the SMART system (BD Biosciences, San Jose, CA). To generate the first strand, polyA RNA was primed with an oligo(dT) primer (5'-ATTCTAGAGGCCGAGGCGGCCGACATG(T)30 VN-3', where V = A, G, or C). The second strand was synthesized by PCR using primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' followed by digestion of the SfiI sites. cDNA was size selected >2kb using 1.1% agarose gel electrophoresis, then ligated into pDNR-LIB. Library is normalized. Library was created and sequencing performed by the DOE Joint Genome Institute (Walnut Creek, CA).

text: DH10B T1-resistant

text: callus

text: Panicum virgatum

text: SfiIA

text: SfiIB

text: CCHY Panicum virgatum callus (N)

text: pDNR-LIB


text: DOE Joint Genome Institute

text: live

text: Generic

text: pool of Cave-in Rock, Shawnee, Pathfinder, NE-NB994, Dacotah, Kanlow, Trailblazer

text: In Vivo Conditions: abiotic and biotic stress treatments. The library was made from oligo(dT)-primed cDNA and cloned into vector pDNR-LIB (Clontech Laboratories, Inc., Mountain View, CA) using the SMART system (BD Biosciences, San Jose, CA). To generate the first strand, polyA RNA was primed with an oligo(dT) primer (5'-ATTCTAGAGGCCGAGGCGGCCGACATG(T)30 VN-3', where V = A, G, or C). The second strand was synthesized by PCR using primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' followed by digestion of the SfiI sites. cDNA was size selected >2kb using 1.1% agarose gel electrophoresis, then ligated into pDNR-LIB. Library is normalized. Library was created and sequencing performed by the DOE Joint Genome Institute (Walnut Creek, CA).

text: DH10B T1-resistant

text: Panicum virgatum

text: SfiIA

text: SfiIB

text: CCHZ Panicum virgatum abiotic and biotic stress treatments (N)

text: pDNR-LIB


text: KRIBB

text: live

text: Generic

text: Bukang

text: Capsicum annuum

text: Pytophthora capsici infected plant

text: pBluescript SK (-)


text: KRIBB

text: live

text: Generic

text: Bukang

characteristic: dev-stage

text: Capsicum annuum

text: Fruit, immature:1.5-7.5cm (+seeds)

text: pBluescript SK (-)


text: KRIBB

characteristic: INSDC center name

text: live

text: Generic

text: Bukang

text: Capsicum annuum

text: Fruit, mature green: pericarp (-seeds)

text: pBluescript SK (-)


text: KRIBB

text: live

text: Generic

text: Bukang

text: Capsicum annuum

text: Fruit, breaker:pericarp (-seeds)

text: pBluescript SK (-)


text: KRIBB

text: live

text: Generic

text: Bukang

text: Capsicum annuum

text: Fruit, mature red ripe: pericarp (-seeds)

text: pBluescript SK (-)


text: KRIBB

text: live

text: Generic

text: Bukang

text: Capsicum annuum

text: Seeds

text: pBluescript SK (-)


text: KRIBB

text: live

text: Generic

text: Bukang

text: Capsicum annuum

text: Flower bud

text: pBluescript SK (-)


text: KRIBB

text: live

text: Generic

text: Bukang

text: Capsicum annuum

text: Open flower

text: pBluescript SK (-)


text: KRIBB

text: live

text: Generic

text: Bukang

text: Capsicum annuum

text: Bark

text: pBluescript SK (-)


text: KRIBB

text: live

text: Generic

text: Bukang

text: Capsicum annuum

text: Peduncle

text: pBluescript SK (-)


text: KRIBB

text: live

text: Generic

text: Bukang

text: Capsicum annuum

text: Callus

text: pBluescript SK (-)


text: KRIBB

text: live

text: Generic

text: Bukang

text: Germination stage 4 & 6 day

text: Capsicum annuum

text: pBluescript SK (-)


text: KRIBB

text: live

text: Generic

text: Bukang

text: Germination stage 18 day

text: Capsicum annuum

text: pBluescript SK (-)


text: KRIBB

text: live

text: Generic

text: Bukang

text: Capsicum annuum

text: rbcS-silenced leaves

text: pBluescript SK (-)


text: Washington University School of Medicine

text: live

text: Generic

text: cDNAs were oligo-dT primed and directionally cloned. Average insert size 1.5 kb, range 0.5-4 kb. Library constructed by A. Zorn and J. Mason (Wellcome/CRC Institute).

text: DH10B (phage-resistant)

text: Xenopus tropicalis

text: NotI

text: EcoRI

text: whole embryo, stages 10-12

text: Wellcome CRC pCS107 tropicalis St10-12

text: plasmid (ampicillin resistant)


text: live

text: Generic

text: DH10B (phage-resistant)

text: Xenopus tropicalis

text: NotI

text: EcoRI

text: egg

text: Wellcome CRC pCS107 tropicalis egg

text: plasmid (ampicillin resistant)


text: Sanger Institute

text: live

text: Generic

text: cDNA was oligo dT primed from 5ug of poly A+ RNA from stages 10-13 gastrulae. EcoRI-NotI cut cDNA was then ligated into pCS107 with EcoRI at the 5' end and NotI at the 3' end.

text: gastrula (stages 10.5-12 mixed)

text: Escherichia coli XL1-blue

text: Xenopus tropicalis

text: EcoRI

text: NotI

text: XGC-gastrula


text: Sanger Institute

text: live

text: Generic

text: cDNA was oligo dT primed from 5ug of poly A+ RNA from neurula. EcoRI-NotI cut cDNA was then ligated into pCS107 with EcoRI at the 5' end and NotI at the 3' end.

text: neurula

text: Escherichia coli DH10B

text: Xenopus tropicalis

text: EcoRI

text: NotI

text: XGC-neurula


text: Sanger Institute

text: live

text: Generic

text: cDNA was oligo dT primed from 5ug of poly A+ RNA from egg. EcoRI-NotI cut cDNA was then ligated into pCS107 with EcoRI at the 5' end and NotI at the 3' end

text: egg

text: Escherichia coli XL1-blue

text: Xenopus tropicalis

text: EcoRI

text: NotI

text: XGC-egg


text: National Cancer Institute / NIH

text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 2.0 kb. Constructed by Invitrogen. Note: This is a Xenopus Gene Collection (XGC) library.

text: embryo, stages 10-13

text: DH10B (phage-resistant)

text: Xenopus tropicalis

text: NotI

text: EcoRV

text: gastrula

text: phagemid (ampicillin resistant)


text: National Cancer Institute / NIH

text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 2.1 kb. Constructed by Invitrogen. Note: This is a Xenopus Gene Collection (XGC) library.

text: embryo, stages 14-19

text: DH10B (phage-resistant)

text: Xenopus tropicalis

text: NotI

text: EcoRV

text: neurula

text: phagemid (ampicillin resistant)


text: National Cancer Institute / NIH

text: live

text: Generic

text: embryo, stages 20-27

text: DH10B (phage-resistant)

text: Xenopus tropicalis

text: NotI

text: EcoRV

text: tailbud

text: phagemid (ampicillin resistant)


text: National Cancer Institute / NIH

text: live

text: Generic

text: embryo, stages 40-45

text: DH10B (phage-resistant)

text: Xenopus tropicalis

text: NotI

text: EcoRV

text: tadpole

text: phagemid (ampicillin resistant)


text: National Cancer Institute / NIH

text: live

text: Generic

text: Bulk tissue was collected from a whole 10 month old male from the F6 strain. 1st strand cDNA was primed with a Not I - oligo(dT) primer, double-stranded cDNA was cloned into the Not I and EcoRV sites of pExpress-1. Library was size-selected for >1.5 kb fragments for an average insert size of 1.92 kb. A normalized version of this library is also available (NICHD_XGC_Swb1N). Library was constructed by Open Biosystems (Huntsville, AL). PLEASE NOTE: This library contains high levels of contamination by worm

text: Xenopus tropicalis

text: EcoRV

text: NotI

text: whole body

text: phagemid (ampicillin resistant)


text: National Cancer Institute / NIH

text: live

text: Generic

text: Bulk tissue was collected from a whole 10 month old male from the F6 strain. 1st strand cDNA was primed with a Not I - oligo(dT) primer, double-stranded cDNA was cloned into the Not I and EcoRV sites of pExpress-1. Library was size-selected for >1.5 kb fragments for an average insert size of 1.92 kb. Library was normalized to Cot5 with a 180-fold reduction of actin. A non-normalized version of this library is also available (NICHD_XGC_Swb1). Library was constructed by Open Biosystems (Huntsville, AL). PLEASE NOTE: This library contains high level of contamination by worm

text: Xenopus tropicalis

text: EcoRV

text: NotI

text: whole body

text: phagemid (ampicillin resistant)


text: National Cancer Institute / NIH

text: live

text: Generic

text: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany).

text: Xenopus tropicalis

text: BamHI

text: XhoI

text: whole embryo, pool of stages 10, 20 and 30

text: phagemid (ampicillin resistant)


text: Sanger Institute

text: live

text: Generic

text: cDNA was oligo dT primed from 5ug of poly A+ RNA from tadpole embryos. EcoRI-NotI cut cDNA was then ligated into pCS107 with EcoRI at the 5' end and NotI at the 3' end

text: tadpole (stage 35-40)

text: E. coli DH10B

text: Xenopus tropicalis

text: EcoRI

text: NotI

text: XGC-tadpole


text: CNRS UMR 8080

text: live

text: Generic

text: Xenopus tropicalis polyA+ RNA was obtained from brain and spinal cord of tadpoles at stages 58-61 and 62-64. cDNAs were synthesized using the SMART system of CLONTECH and directionally cloned into pCMVSPORT6_xtbs, a modified version of pCMVSPORT6 allowing directional cloning using asymetric SfiI sites. For antisense RNA synthesis, use T7 promoter and for sense RNA use SP6 promoter. Library constructed by Dr. L. Coen and Prof. B. Demeneix (Museum National d'Histoire Naturelle and CNRS UMR 5166, Paris, France).

text: metamorphosis stage 58-64

text: E.coli DH10B

text: Xenopus tropicalis

text: SfiI

text: SfiI

text: Ivory Coast

characteristic: strain

text: pool of brains and spinal cords from tadpoles

text: Xenopus tropicalis xtbs plasmid library

text: Plasmid


text: CNRS UMR 8080

characteristic: INSDC first public

text: live

text: Generic

text: Xenopus tropicalis polyA+ RNA was obtained from pool of heads and retinas from tailbud stages 25-35 cDNAs were synthesized using the SMART system of CLONTECH and directionally cloned into pCMVSPORT6_xthr, a modified version of pCMVSPORT6 allowing directional cloning using asymetric SfiI sites. For antisense RNA synthesis, use T7 promoter and for sense RNA use SP6 promoter. Library constructed by Drs. N. Pollet, M. Perron, M. Wegnez, A. Mazabraud (CNRS UMR 8080, Universite Paris Sud, Orsay, France).

text: stage 25-35

text: E.coli DH10B

text: Xenopus tropicalis

text: SfiI

text: SfiI

text: Ivory Coast

text: Xenopus tropicalis xthr plasmid library

text: Plasmid


text: Sanger Institute

characteristic: INSDC last update

text: live

text: Generic

text: cDNA was oligo dT primed from 5ug of poly A+ RNA from tailbud. EcoRI-NotI cut cDNA was then ligated into pCS107 with EcoRI at the 5' end and NotI at the 3' end.

text: tailbud (stage 28-30)

text: Escherichia coli DH10B.

text: Xenopus tropicalis

text: EcoRI

text: NotI

text: XGC-tailbud


text: Sanger Institute

text: live

text: Generic

characteristic: NCBI submission model

text: cDNA was oligo dT primed from 5ug of poly A+ RNA from tailbud head. EcoRI-NotI cut cDNA was then ligated into pCS107 with EcoRI at the 5' end and NotI at the 3' end.

text: tailbud head (stage 28-30)

text: Escherichia coli DH10B.

text: Xenopus tropicalis

text: EcoRI

text: NotI

text: XGC-tailbud-head


text: DOE Joint Genome Institute

text: live

text: Generic

characteristic: NCBI submission package

text: Tadpole library constructed by Russell B. Fletcher in R. Harland's lab using poly A RNA and oligo dT primers (Invitrogen SuperScript Plasmid System for cDNA Synthesis and Cloning). SalI (5' end) -NotI (3' end) cDNA was inserted into vector pCS108 (http://mcb.berkeley.edu/labs/harland/pages/plasmids.html).

text: Tadpole (st. 36-41)

text: E. coli XL1-Blue derivative, Stratagene ElectroTen-Blue

text: Xenopus tropicalis

text: SalI

text: NotI

text: whole embryo


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was prepared from 5 ug of poly A+ RNA by oligo-dT priming (V(T)20CTCGAGAGAGAGAGAG). After ligation of PstI adapters and by XhoI and PstI digestion, the cDNA was size selected by chromatography on Sepharose CL-4B columns and fractions containing cDNAs larger than 500 bp were ligated into PstI/XhoI-digested pCS22+. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Jisong Peng and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).

text: Gastrula+Neurula (st. 10-13, 15-18)

text: TOP10 E. coli

text: Xenopus tropicalis

characteristic: organism

text: PstI

text: XhoI

text: N3 (Nigerian 3rd generation inbred)

text: whole embryo


text: DOE Joint Genome Institute

text: live

text: Generic

text: Gastrula library constructed by Russell B. Fletcher in R. Harland's lab using poly A RNA and oligo dT primers (Invitrogen SuperScript Plasmid System for cDNA Synthesis and Cloning). SalI (5' end) -NotI (3' end) cDNA was inserted into vector pCS108 (http://mcb.berkeley.edu/labs/harland/pages/plasmids.html).

text: Gastrula (st. 10.5-12.5)

text: Xenopus tropicalis

text: SalI

text: NotI

text: whole embryo

characteristic: title


text: DOE Joint Genome Institute

text: live

text: Generic

text: Gastrula (st. 10-13)

text: TOP10 E. coli

text: Xenopus tropicalis

text: PstI

text: XhoI

text: N6 (Nigerian 6th generation inbred)

text: whole embryo

characteristic: tissue-type


text: DOE Joint Genome Institute

text: live

text: Generic

text: Neurula (st. 14-18)

text: TOP10 E. coli

text: Xenopus tropicalis

text: PstI

text: XhoI

text: N6 (Nigerian 6th generation inbred)

text: whole embryo

characteristic: vector


text: DOE Joint Genome Institute

text: live

text: Generic

text: This library was made from dT primed cDNA and cloned into Invitrogen pCMVsport6 vector. The work was done at DOE Joint Genome Institute. Poly A RNA were primed with 5' GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT 3'. CDNA were ligated to SalI adapter (5' TCGACCCACGCGTCCG and 5'CGGACGCGTGGG), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into NotI and SalI digested pCMVsport6 vector.

characteristic: description

text: Adult

text: ElectroMAX DH10B

text: Xenopus tropicalis

text: SalI

text: NotI

text: Brain


text: DOE Joint Genome Institute

text: live

characteristic: INSDC status

text: Generic

text: Adult

text: ElectroMAX DH10B

text: Xenopus tropicalis

text: SalI

text: NotI

text: male

characteristic: sex

text: Testes


characteristic: External Id

text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was prepared from 5 ug of poly A+ RNA by oligo-dT priming (5'-GAGAGAGAGAGAGAGAGAGACTAGTCTCGAGTTTTTTTTTTTTTTTTTT-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).

text: Adult

text: ElectroMAX DH10B T1 Phage Resistant cells

text: Xenopus tropicalis

text: EcoRI

text: XhoI

text: N6 (Nigerian 6th generation inbred)

text: Intestine


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was prepared from 5 ug of poly A+ RNA by oligo-dT priming (5'-ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTTTTTV-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).

text: Adult

text: Xenopus tropicalis

text: EcoRI

text: XhoI

text: N6 (Nigerian 6th generation inbred)

text: Heart


text: DOE Joint Genome Institute

text: live

text: Generic

text: Adult

text: Xenopus tropicalis

text: EcoRI

text: XhoI

text: male

text: N6 (Nigerian 6th generation inbred)

text: Liver


text: DOE Joint Genome Institute

text: live

text: Generic

text: Metamorphic (st.62)

text: ElectroMAX DH10B

text: Xenopus tropicalis

text: SalI

text: NotI

text: whole embryo


text: DOE Joint Genome Institute

text: live

text: Generic

text: This library was made from dT primed cDNA and cloned into Invitrogen pCMVsport6 vector. The work was done at DOE Joint Genome Institute. Poly A RNA were primed with 5' GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT 3'. CDNA were ligated to SalI adapter (5' TCGACCCACGCGTCCG and 5'CGGACGCGTGGG), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into NotI and SalI digested pSPORT1 vector.

text: Metamorphic (st.64)

text: ElectroMAX DH10B

text: Xenopus tropicalis

text: SalI

text: NotI

text: whole embryo


text: DOE Joint Genome Institute

text: live

text: Generic

text: Metamorphic (st.62)

text: ElectroMAX DH10B

text: Xenopus tropicalis

text: SalI

text: NotI

text: whole embryo


text: DOE Joint Genome Institute

text: live

text: Generic

text: Adult

text: Xenopus tropicalis

text: EcoRI

text: XhoI

text: N6 (Nigerian 6th generation inbred)

text: Kidney


text: DOE Joint Genome Institute

text: live

text: Generic

text: Adult

text: Xenopus tropicalis

text: EcoRI

text: XhoI

text: N6 (Nigerian 6th generation inbred)

text: Fat body


text: DOE Joint Genome Institute

text: live

text: Generic

text: Adult

text: Xenopus tropicalis

text: EcoRI

text: XhoI

text: N6 (Nigerian 6th generation inbred)

text: Lung


text: DOE Joint Genome Institute

text: live

text: Generic

text: Adult

text: Xenopus tropicalis

text: EcoRI

text: XhoI

text: female

text: N6 (Nigerian 6th generation inbred)

text: Ovary


text: DOE Joint Genome Institute

text: live

text: Generic

text: Adult

text: Xenopus tropicalis

text: EcoRI

text: XhoI

text: N6 (Nigerian 6th generation inbred)

text: Stomach


text: DOE Joint Genome Institute

text: live

text: Generic

text: Adult

text: Xenopus tropicalis

text: EcoRI

text: XhoI

text: N6 (Nigerian 6th generation inbred)

text: Skeletal Muscle


text: DOE Joint Genome Institute

text: live

text: Generic

text: Adult

text: Xenopus tropicalis

text: EcoRI

text: XhoI

text: female

text: N6 (Nigerian 6th generation inbred)

text: Oviduct


text: DOE Joint Genome Institute

text: live

text: Generic

text: Adult

text: Xenopus tropicalis

text: EcoRI

text: XhoI

text: N6 (Nigerian 6th generation inbred)

text: Skin


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was prepared from total RNA by oligo-dT priming (5'-ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTTTTTV-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).

text: Adult

text: Xenopus tropicalis

text: EcoRI

text: XhoI

text: N6 (Nigerian 6th generation inbred)

text: Spleen


text: DOE Joint Genome Institute

text: live

text: Generic

text: Adult

text: Xenopus tropicalis

text: EcoRI

text: XhoI

text: N6 (Nigerian 6th generation inbred)

text: Brain


text: DOE Joint Genome Institute

text: live

text: Generic

text: Adult

text: Xenopus tropicalis

text: EcoRI

text: XhoI

text: male

text: N6 (Nigerian 6th generation inbred)

text: Testes


text: DOE Joint Genome Institute

text: live

text: Generic

text: Metamorphic (st.64)

text: ElectroMAX DH10B

text: Xenopus tropicalis

text: SalI

text: NotI

text: whole embryo


text: National Cancer Institute / NIH

text: live

text: Generic

text: cDNA was primed using oligo-dT primer: GACTAGTTCTAGATCGCGAGCGGCCGCC(T)25 and cloned into the SmaI/NotI sites of pCS111. Size-selection >1.25kb resulted in an average insert size of 1.9 kb. This primary library was constructed by Express Genomics (Frederick, MD). Note: this is a (http://xgc.nci.nih.gov/) Xenopus Gene Collection library.

text: DH10B TonA

text: small intestine

characteristic: organ

text: Xenopus tropicalis

text: SmaI

text: NotI

text: small intestine, 6 pooled

text: plasmid


text: National Cancer Institute / NIH

text: live

text: Generic

text: DH10B TonA

characteristic: lab-host

text: small intestine

text: Xenopus tropicalis

text: SmaI

text: NotI

text: small intestine, 5 pooled

text: plasmid


text: National Institute for Basic Biology

text: live

text: Generic

text: gastrula

characteristic: dev-stage

text: Xenopus tropicalis

text: embryo

text: Xenopus tropicalis embryo gastrula


text: DOE Joint Genome Institute

characteristic: INSDC center name

text: live

text: Generic

text: This library was made from dT primed cDNA and cloned into pCS107 vector. The work was done at DOE Joint Genome Institute. Poly A RNA were primed with 5' GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT 3'. CDNA were ligated to SalI adapter (5' TCGACCCACGCGTCCG and 5'CGGACGCGTGGG), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into NotI and SalI digested pCS107 vector.

text: Adult

text: Xenopus tropicalis

text: SalI

text: NotI

text: TGA IC

text: Bone


text: National Cancer Institute / NIH

text: live

text: Generic

text: cDNA was primed using oligo-dT primer: GACTAGTTCTAGATCGCGAGCGGCCGCC(T)25 and cloned into the SmaI/NotI sites of pCS111. Size-selection >1.25kb resulted in an average insert size of 1.7 kb. This primary library was constructed by Express Genomics (Frederick, MD). Note: this is a (http://xgc.nci.nih.gov/) Xenopus Gene Collection library.

text: DH10B TonA

text: small intestine

text: Xenopus tropicalis

text: SmaI

text: NotI

text: small intestine, 5 pooled

text: plasmid


text: National Cancer Institute / NIH

text: live

text: Generic

text: cDNA was primed using oligo-dT primer: GACTAGTTCTAGATCGCGAGCGGCCGCC(T)25 and cloned into the SmaI/NotI sites of pCS111. Size-selection >1.25kb resulted in an average insert size of 2.1 kb. This primary library was constructed by Express Genomics (Frederick, MD). Note: this is a (http://xgc.nci.nih.gov/) Xenopus Gene Collection library.

text: DH10B TonA

text: small intestine

text: Xenopus tropicalis

text: SmaI

text: NotI

text: small intestine, 7 pooled

text: plasmid


text: DOE Joint Genome Institute

text: live

text: Generic

text: Adult

text: Xenopus tropicalis

text: SalI

text: NotI

text: Thymus


text: DOE Joint Genome Institute

text: live

text: Generic

text: Metamorphic

text: Xenopus tropicalis

text: SalI

text: NotI

text: TGA IC

characteristic: strain

text: Limb


text: DOE Joint Genome Institute

characteristic: INSDC first public

text: live

text: Generic

text: Adult

text: ElectroMAX DH10B T1 Phage Resistent cells

text: Xenopus tropicalis

text: SalI

text: NotI

text: Spleen


text: DOE Joint Genome Institute

characteristic: INSDC last update

text: live

text: Generic

text: Adult

text: Xenopus tropicalis

text: SalI

text: NotI

text: Nigerian

text: Pancreas


text: DOE Joint Genome Institute

text: live

text: Generic

characteristic: NCBI submission model

text: Metamorphic

text: Xenopus tropicalis

text: SalI

text: NotI

text: TGA IC

text: Tail


text: DOE Joint Genome Institute

text: live

text: Generic

characteristic: NCBI submission package

text: Adult

text: Xenopus tropicalis

text: SalI

text: NotI

text: TGA IC

text: Testes


text: DOE Joint Genome Institute

text: live

text: Generic

text: Stage 25

text: Xenopus tropicalis

characteristic: organism

text: SalI

text: NotI

text: PopA

text: whole


text: DOE Joint Genome Institute

text: live

text: Generic

text: Poly(A)+RNA was isolated from 58 ug of total RNA by one round of oligo(dT) selection with oligo(dT) coated magnetic particles (Seradyn, Inc.). From 1.2 ug of this poly(A)+RNA mRNA, a cDNA library was constructed by using an oligo dT primer-adapter containing a Not I site and Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) to prime and synthesize first strand cDNA. After the second strand was synthesized, the double stranded cDNA was digested with Not I, size fractionated (>1.4 kb) and cloned directionally into the Not I and Sma I sites of the pCS111 vector. From one bulk ligation (300 ng of pCS111 vector, Not I-Sma I cut, and 120 ng of Not I digested cDNA per 120 ul of ligation), followed by electroporation into T1 phage resistant E. coli, 1.2 x 107 primary clones were produced. The cDNA inserts from 24 randomly picked clones were sized by digestion with Not I and Eco RI. The average insert size of these 24 clones was 1.9 kb.

text: Adult

text: E. coli DH10B T1 Phage-resistant

text: Xenopus tropicalis

text: SmaI

text: NotI

text: Eye

characteristic: title


text: Washington University School of Medicine

text: live

text: Generic

text: Sterkiella histriomuscorum

text: HincII

text: HincII

text: plasmid

text: pSMART


text: live

text: Generic

text: Sterkiella histriomuscorum

text: HincII

text: HincII

text: plasmid

text: pSMART


text: live

text: Generic

text: Mating Type 0hr

text: Lucigen E.cloni 10G Supreme

text: Sterkiella histriomuscorum

text: HincII

text: HincII

text: plasmid

text: pSMART


text: live

text: Generic

text: Mating Type 5hr

text: Lucigen E.cloni 10G Supreme

text: Sterkiella histriomuscorum

text: HincII

text: HincII

text: plasmid

text: pSMART


text: live

text: Generic

text: Mating Type 13hrs

text: Lucigen E.cloni 10G Supreme

text: Sterkiella histriomuscorum

text: HincII

text: HincII

text: plasmid

text: pSMART


text: live

text: Generic

text: Mating Type 21hr

text: Lucigen E.cloni 10G Supreme

text: Sterkiella histriomuscorum

text: HincII

text: HincII

text: plasmid

text: pSMART


text: live

text: Generic

text: Mating Type 30hr

text: Lucigen E.cloni 10G Supreme

text: Sterkiella histriomuscorum

text: HincII

text: HincII

text: plasmid

text: pSMART


text: China Agricultural University

text: live

text: Generic

text: Chrysanthemum x morifolium

text: Subtracted cDNA library of chrysanthenmum DREB1A downstream genes


text: Osmania University

text: live

text: Generic

characteristic: cultivar

text: pigeonpea plants of 4 weeks old were subjected to PEG stress. Total RNA was isolated from leaf and root tissues. Subtracted cDNA library was constructed.

text: four week old plants

text: Escherichia coli

text: leaf and root

text: Cajanus cajan

text: Kpn 1

text: Sac 1

text: hermophrodite

text: leaf and root tissue

characteristic: tissue-type

text: Pigeonpea PEG stress induced cDNA library

text: Plasmid

text: pBSSK+


text: USDA, ARS

text: live

text: Generic

text: Illinwake Red

text: Tester: a mixture of flesh fruits at green (12 DAP), mature (24 DAP) and ripe (36 DAP) stages; Driver: young leaf.

characteristic: description

text: mixed fruits at early stage(12 days after pollination; DAP), ripening stage (24 DAP) and ripe fruit (36 DAP)

text: Citrullus lanatus

text: 5' EcoRI

text: 3' NotI

text: flesh fruit

text: Watermelon fruit normalization and subtraction library

text: Plasmid

text: pBlueScript II (+)

characteristic: vector


text: IEW

text: live

characteristic: INSDC status

text: Generic

text: Orchesella cincta

text: mixed

characteristic: sex

text: lab culture

text: Gut library Thierry Janssens


characteristic: External Id

text: CSIR Biosciences

text: live

text: Generic

text: RNA was extracted from leaves using a Plant RNeasy Kit (Qiagen). mRNA was purified from total RNA with an Oligotex mRNA Mini Kit (Qiagen). Double stranded cDNA was synthesised using a cDNA Synthesis System (Roche). Differentially expressed cDNAs were isolated from treated leaves using a PCR-Select cDNA Subtraction Kit (BD Biosciences, Clontech). cDNA enriched for differentially expressed transcripts was cloned into pGEMT-Easy, and transformed into E. coli DH10B cells.

text: Ten day old seedlings

text: E. coli DH10B

text: Cenchrus americanus

text: leaves

text: Ten day old pearl millet seedlings treated with defence elicitors

text: Plasmid

text: pGEMT-Easy


text: Univeristy Health Network

text: live

text: Generic

text: Adult

text: Mustela putorius furo

text: spleen, liver, thymus

text: Ferret library from spleen, liver, and thymus


text: Universita' degli Studi di Padova

text: live

text: Generic

text: Jemalong 2HA

text: cDNA-AFLP library using TA cloning method for insertion into plasmid

text: adult nodulated plant

text: XL1 Blue

characteristic: lab-host

text: leaf

characteristic: organ

text: Medicago truncatula

text: barrel medic leaf senescence cDNA-AFLP library

text: Plasmid

text: pGEM-Teasy


text: Northwest A & F University

characteristic: INSDC center name

text: live

text: Generic

text: aborting bud

text: Brassica rapa subsp. pekinensis

text: aborting bud

text: aborting bud of chinese cabbage


text: INRA - INSA de Lyon

text: live

text: Generic

text: Suppressive subtractive hybridization (SSH) technology was applied to cDNA from E. coli infected larvae and cDNA from naive larvae. The cDNA substracted library was made using a PCR-selected cDNA subtraction kit (Clontech). Sized cDNAs were directly ligated into pCR2.1 TOPO. T7 and M13rev primers were used for EST sequencing. WARNING: the library may contains a small percentage of contamination (rDNA, bacterial DNA...); Differential gene expression between naive and E. coli infected aphids was not confirmed.

text: Aposymbiotic fourth instar larvae (Rifampicin-treated aphids)

characteristic: dev-stage

text: Escherichia coli TOP10

text: Whole organism

text: Acyrthosiphon pisum

text: LL01 (Lusignan, France)

characteristic: strain

text: Aphid Immune Substracted library

text: Plasmid

text: pCR2.1 TOPO


text: University of Delhi South Campus

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Library was constructed from mRNA using Clontech PCR Select cDNA subtraction kit following manufacturer's instructions.

text: Developing Seed at 7, 14, 21 and 30 DPA

text: E.coli DH5a

text: Triticum aestivum

text: Developing seed

text: Triticum aestivum developing seed heat stress forward subtractive library

text: Plasmid

text: pGEMTeasy


text: University of Delhi South Campus

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Developing seed at 7, 14,21 and 30 DPA

text: E.coli DH5a

text: Triticum aestivum

text: Developing seed

text: Triticum aestivum developing seed heat stress reverse subtractive library

text: Plasmid

text: pGEMTeasy


text: University of Delhi South Campus

text: live

text: Generic

text: Flower part anther and ovary

text: E.coli DH5a

text: Triticum aestivum

characteristic: organism

text: Flower

text: Triticum aestivum flower heat stress forward subtractive library

characteristic: title

text: Plasmid

text: pGEMTeasy


text: University of Delhi South Campus

text: live

text: Generic

text: 10-day-old seedling

text: E.coli DH5a

text: Triticum aestivum

text: Shoot

characteristic: tissue-type

text: Triticum aestivum 10-day-old seeding heat stress forward subtractive library

text: Plasmid

text: pGEMTeasy


text: Oklahoma State University

text: live

text: Generic

characteristic: cultivar

text: Suppression subtraction hybridization library

characteristic: description

text: Medicago truncatula

text: Leaf

text: Medicago truncatula cv. JE154 acute ozone stress


text: Oklahoma State University

text: live

characteristic: INSDC status

text: Generic

text: Jemalong

text: Medicago truncatula

text: Leaf

text: Medicago truncatula cv. Jemalong acute ozone stress


text: National Institute of Biology

text: live

text: Generic

text: Sante

text: PVY-inoculated plants and mock-inoculated plants were grown for 12 hours. ClonTech protocol was followed for preparation of the subtraction libraries.

text: 12 hours

text: XL-1 blue ultracompetent cells (Stratagene)

text: Leaf

text: Solanum tuberosum

text: Potato cv. Sante subtractive library of genes upregulated after PVY-NTN inoculation

text: Plasmid

text: pGEM-T easy

characteristic: vector


characteristic: External Id

text: National Institute of Biology

text: live

text: Generic

text: Sante

text: 12 hours

text: Leaf

text: Solanum tuberosum

text: Potato cv. Sante subtractive library of genes downregulated after PVY-NTN inoculation

text: Plasmid

text: pGEM-T easy


text: USDA-ARS

text: live

text: Generic

text: total RNA was purified by TRIZOL Reagent (Gibco BRL Life Technologies); cDNA library was constructed using Creator SMART cDNA Library Construction Kit (Clontech) (cat. # 634903)

text: early 4th instar larvae

text: Megachile rotundata

text: SfiIA

text: SfiIB

text: healthy larvae

text: HB, healthy alfalfa leafcutting bee library

text: Plasmid

text: pDNR-LIB


text: USDA-ARS

text: live

text: Generic

text: early 4th instar larvae

text: Megachile rotundata

text: SfiIA

text: SfiIB

text: infected larvae

text: IB, infected alfalfa leafcutting bee library

text: Plasmid

text: pDNR-LIB


text: University of Queensland

text: live

text: Generic

text: Stages: egg; gastrula; early trochophore; mid torsion; early veliger, mid veliger; early competent veliger; later competent veliger; 1 day post metamorphosis; 5 days post metamorphosis; juvenile. A directionally cloned cDNA library was constructed using a BD Biosciences SMART library construction kit. Phage were converted into plasmid DNA following manufacturer's instructions prior to standard plasmid DNA isolation and sequencing protocols

text: mixed

text: Haliotis asinina

text: SfiIA

text: SfiIB

text: whole animal; mantle

text: Haliotis asinina developmental microarray library

text: lambda triplex


text: NRC Plant Biotechnology Institute

text: live

text: Generic

text: Nugget

text: The library HLUTR3CH was constructed from RNA isolated from lupulin glands from the variety 'Nugget'. Hop cones were collected near Baasdorf, Germany in August 2005. Lupulin glands were isolated by agitating dissected cones in liquid nitrogen followed by filtration to remove cone material. Total RNA was isolated using an RNeasy Plant Mini Kit (Qiagen) and mRNA was purified using a Dynabeads mRNA purification kit (Qiagen). The library was constructed using a cDNA library synthesis kit (Stratagene) with cDNAs ligated into the plasmid vector pBluescript SK+. HLUTR3CH was normalized using a hydroxyapatite-based method (C0t 2.5 and 5) (Bonaldo et al. (1996) Genome Res. 6, 791).

text: Trichomes isolated from mid to ripe stage hop cones

text: Humulus lupulus

text: EcoRI

text: XhoI

text: Female

characteristic: sex

text: Trichome (lupulin gland)

text: Phage

text: pBluescript SK+


text: NRC Plant Biotechnology Institute

text: live

text: Generic

text: Taurus

text: The library HLUPJN1 was constructed from RNA isolated from lupulin glands from the variety 'Taurus'. Hop cones were collected near Mainburg, Germany in August 2002-2003. Lupulin glands were isolated by agitating dissected cones in liquid nitrogen followed by filtration to remove cone material. Total RNA was isolated using buffered phenol-chloroform-isoamyl alcohol followed by a clean-up with an RNeasy Plant Mini Kit (Qiagen); mRNA was purified using an Oligotex kit (Qiagen). The library was constructed using a SMART kit (Clontech). cDNAs were ligated into the phage vector lambda-TriplEx2 and packaged with MaxPlax Lambda packaging extract (Epicentre Technologies). For sequencing, cDNA inserts were PCR amplified from phage plaques using the vector primers 5'-CAAGCTCCGAGATCTGGACGAGC-3 and 5'-ATACGACTCACTATAGGGCGAATTGGCC-3'.

text: Humulus lupulus

text: SfiIA

text: SfiIB

text: Phage

text: lambda-TriplEx2 (Clontech)


text: NRC Plant Biotechnology Institute

text: live

text: Generic

text: Nugget

text: The library HLUTR2CH was constructed from RNA isolated from lupulin glands from the variety 'Nugget'. Hop cones were collected near Baasdorf, Germany in August 2005. Lupulin glands were isolated by agitating dissected cones in liquid nitrogen followed by filtration to remove cone material. Total RNA was isolated using an RNeasy Plant Mini Kit (Qiagen) and mRNA was purified using a Dynabeads mRNA purification kit (Qiagen). The library was constructed using a cDNA library synthesis kit (Stratagene) and cDNAs ligated into the plasmid vector pBluescript SK+.

text: Humulus lupulus

text: EcoRI

text: XhoI

text: Phage

text: pBluescript SK+


text: NRC Plant Biotechnology Institute

text: live

text: Generic

text: Taurus

text: The library HLUPLC was constructed from RNA isolated from lupulin glands from the variety 'Taurus'. Hop cones were collected near Mainburg, Germany in August 2003. Lupulin glands were isolated by agitating dissected cones in liquid nitrogen followed by filtration to remove cone material. Total RNA was isolated using an RNeasy Plant Mini Kit (Qiagen) and mRNA was purified using a Dynabeads mRNA purification kit (Qiagen). HLUPLC was constructed using a cDNA library synthesis kit (Stratagene) and cDNAs ligated into the plasmid vector pBluescript SK+. The library was normalized using a hydroxyapatite-based method (C0t 2.5 and 5) (Bonaldo et al. (1996) Genome Res. 6, 791).

text: Humulus lupulus

text: EcoRI

text: XhoI

text: Female

text: Trichome (lupulin gland)

text: HLUPLC

text: pBluescript SK+


text: University of California, San Francisco

text: live

text: Generic

text: mRNA was ligated to the Gene Racer oligo (Invitrogen), reverse-transcribed, and amplified using SHANK3-specific reverse primers with PfuUltra high-fidelity DNA polymerase (Stratagene) under the following 3-step 'touch-down' cycling parameters: (1) 5 cycles of 94oC for 30 sec, 72oC for 1 min, (2) 5 cycles of 94oC for 30 sec, 70oC for 1 min, (3) 30 cycles of 94oC for 30 sec, 62oC for 30 sec, and 72oC for 1 min, followed by 72oC for 10 min. The amplification products were gel purified, cloned into the pCR4-TOPO vector (Invitrogen), and inserts were sequenced.

text: post-natal day 56

text: brain

characteristic: organ

text: Mus musculus

text: male

text: whole brain

text: Mouse post-natal day 56 brain 5'-RACE


text: Flinders University

characteristic: INSDC center name

text: live

text: Generic

text: Genes that were differentially expressed in the hypobranchial gland of D. orbita. Suppressive Subtractive Hybridization was used to create this differentially expressed cDNA library, using hypobranchial gland tissue as tester cDNA and mantle tissue cDNA as the reference cDNA.

text: hypobranchial gland

text: Dicathais orbita

text: Dicathais orbita hypobranchial gland library

text: Plasmid

text: pGEM-Teasy Vector


text: Flinders University

text: live

text: Generic

text: Genes expressed in the mantle tissue of the marine snail Dicathais orbita. Used as reference cDNA in the Suppressive subtractive hybridization of hypobranchial gland genes from D. orbita

text: hypobranchial gland

text: Dicathais orbita

text: Dicathais orbita mantle library

text: Plasmid

text: pGEM-Teasy Vector


text: Beijing Academy of Agriculture and Forestry Science

text: live

text: Generic

text: mesophyll cell

characteristic: cell type

text: cDNA-AFLP analysis was carried out to discover vernalization-related EST drived by low-temperature

text: vernalization-related

characteristic: dev-stage

text: Brassica rapa

text: hermaphrodite

text: leaves

text: Brassica rapa vernalization-related cDNA-AFLP


text: University of Groningen

characteristic: INSDC first public

text: live

text: Generic

text: AFLP-cDNA technique. Double-stranded cDNA was synthesized from 20 ug ovarian RNA. cDNA was digested with TaqI and MseI restriction enzymes. After adaptor ligation, preamplification was done using the corresponding primer for the enzyme adaptor sequence. The preamplification products were selectively amplified using the preamplification primers with an extra 2 or 3 nucleotide extension at the 3' end for the TaqI and MseI primer respectively.

text: adult

text: Venturia canescens

text: female

text: Arrhenotokous, Thelytokous

characteristic: strain

text: ovaries


text: Crops Research Centre, Teagasc

characteristic: INSDC last update

text: live

text: Generic

characteristic: NCBI submission model

text: immature stigma Z incompatible library

text: Lolium perenne subsp. perenne

text: stigma

text: IM-Z-SI


text: Crops Research Centre, Teagasc

text: live

text: Generic

characteristic: NCBI submission package

text: immature stigma S incompatible library

text: Lolium perenne subsp. perenne

characteristic: organism

text: stigma

text: IM-S-SI


text: Graduate School of Fisheries Science, Hokkaido University

text: live

text: Generic

text: This library was prepared using testis samples of normal diploid subtracted from those of clonal diploid to identify genes up-regulated in spermatogenesis of normal diploid. This was performed using the SuperSmart PCR cDNA Synthesis kit and PCR-selected cDNA Subtraction kit (Clontech) according to the manufacturer's protocols. After the secondary PCR, the products were inserted into pCR 4-TOPO (Invitorogen).

text: Misgurnus anguillicaudatus

text: testis

characteristic: tissue-type

text: Misgurnus anguillicaudatus normal-clone subtractive cDNA library

characteristic: title


text: live

text: Generic

text: This library was prepared using testis samples of clonal diploid subtracted from those of normal diploid to identify genes up-regulated in spermatogenesis of clonal diploid. This was performed using the SuperSmart PCR cDNA Synthesis kit and PCR-selected cDNA Subtraction kit (Clontech) according to the manufacturer's protocols. After the secondary PCR, the products were inserted into pCR 4-TOPO (Invitorogen).

characteristic: description

text: Misgurnus anguillicaudatus

text: testis

text: Misgurnus anguillicaudatus clone-normal subtractive cDNA library


text: National Institute of Agrobiological Sciences

text: live

characteristic: INSDC status

text: Generic

text: 4th molting stage

text: Bombyx mori

text: mixed

text: epidermis

text: epM-


text: Ministry of Education, Huazhong Agricultural University P.R.C.

text: live

text: Generic

text: P. cubensis resistance cucumber inbred material

characteristic: cultivar

text: Pseudoperonospora cubensis (Berk. and Curk.) Rostov (P. cubensis)-challenged leaf Suppression Subtractive Hybridization cDNA library of cucumber seedlings. Detached leaves were challenged with 1x105 sporangia/ml P. cubensis. Leaf tissue was collected at 32, 48, 56 hours post-challenge respectively, and frozen in liquid nitrogen immediately. Detached leaves sprayed with water were used as controls.

text: two second leaf stage

characteristic: lab-host

text: Cucumis sativus

text: SmalI

text: NotI

text: leaf

text: SSH cDNA library of cucumber seedlings challenged with P. cubensis

text: BlueScript M13 (+) SK

characteristic: vector


characteristic: External Id

text: Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences

text: live

text: Generic

text: Flower buds and flower were collected in four stage from May to June 2008: 1) flower bud stage: length of flower bud was 1cm, 2cm, 3cm, 4cm, 5cm, 6cm, 7cm, 8cm, 9cm, 10cm, 11cm and 12cm .2) beginning of flowering period.3) full blooming stage.4) end of flowering stage.

text: Lilium regale

text: flower

text: Lilium regale Wils Flower full-length cDNA Library

text: Lambda TriplEx2


text: Huazhong Agricultural University

characteristic: INSDC center name

text: live

text: Generic

text: suppression subtractive hybridization library of genes involved in cotton response to Verticillium dahliae

text: root infected after 0-96hours

text: Gossypium barbadense

text: root

text: SSH library of genes involved in cotton response to Verticillium dahliae


text: College of Horticulture, Northwest A&F University

text: live

text: Generic

text: Growing stage

text: E.coli JM109

text: Camellia sinensis

text: SfiI A

text: SfiI B

text: Young leaf

text: Young leaves cDNA library of tea plant (Camellia sinensis)

text: pBluescript II SK