text: Stanford Human Genome Center

text: live

text: Generic

characteristic: NCBI submission model

text: mRNA was prepared from a collection of California mussels that had been exposed to a variety of environmental challenges that included heat, cold, hypoxia, emersion, heavy metal and salinity stress. The cDNA library was constructed using Cap-trapping methodology developed by RIKEN. Cap-trapped 1st strand cDNA was produced and hybridized with several biotinylated RNA drivers including: starting mRNA as the normalizing driver, and run-off transcripts from mini-libraries containing highly expressed genes, and previously sequenced cDNAs as subtracting drivers. cDNA that did not hybridize to the biotinylated driver was isolated, directionally-cloned into lambda FLC-I vector (from RIKEN), and plasmids excised using an in vivo liquid excision method.

text: DH10B (T1 phage resistant)

text: Mytilus californianus

text: GGCCAAATCGGCC

text: GGCCCTTATGGCC

text: Adductor muscle

text: SHGC-MUT

text: Plasmid

text: pFLC-I


status: PUBLIC

characteristic: External Id

text: Agrochemistry institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission package

text: Hogland nutrition solution

characteristic: description

text: Leaf

tag: attribute

characteristic: organ

text: Sedum alfredii

characteristic: organism

text: TaqI

text: AseI

text: Sedum alfredii Hance cDNA-AFLP library

characteristic: title

text: pGEM-T EASY

characteristic: vector


text: Research Institute for the Biology of Farm Animals

text: live

text: Generic

text: cDNA fragments obtained by heterologous PCR

text: 7-8 month, adult

characteristic: dev-stage

text: lymph node

text: Ovis aries

text: female

characteristic: sex

text: texel sheep

characteristic: strain

text: retropharyngeal lymph node

characteristic: tissue-type

text: texel sheep partial heterologous PCR in lymph node


text: SINTEF Materials and Chemistry

text: live

text: Generic

text: Copepod subtraction library from whole copepods exposed to oil, amine, copper and elevated temperature

text: Adult

text: Calanus finmarchicus

text: Whole organism

text: Calanomics library 1


text: Oregon State University

text: live

text: Generic

text: The cDNA library was obtained from Douglas-fir seedlings in their first year of growth. All seedlings were grown outdoors in Corvallis, Oregon, USA and collected from September 2003 to November 2003. Total RNA was extracted from ~3g of ground tissue (separately from buds, stems, and needles) from a sample of 10 seedlings, following a modified protocol described in Chang et al. (1993). Total RNA was used by Evrogen JCS (Moscow, Russia) for ds cDNA synthesis, using the SMART approach (Zhu et al. 2001), and normalized using the DSN normalization method (Zhulidov et al. 2004). cDNA was directionally inserted into the pAL17.1 vector and transformed into E. coli with the BioRad Micropulser (Hercules, CA). cDNA clones were sequenced by SymBio Corporation (Menlo Park, CA).

text: seedling

text: E. coli

characteristic: lab-host

text: Pseudotsuga menziesii var. menziesii

text: AscI

text: NotI

text: needles, stems, and buds

text: Douglas-fir cold acclimating cDNA library 2003-2004 (CA_2003-04)


text: Oregon State University

text: live

text: Generic

characteristic: NCBI submission model

text: The cDNA library was obtained from Douglas-fir seedlings in their first year of growth. All seedlings were grown outdoors in Corvallis, Oregon, USA and collected from December 2003 to January 2004. Total RNA was extracted from ~3g of ground tissue (separately from buds, stems, and needles) from a sample of 10 seedlings, following a modified protocol described in Chang et al. (1993). Total RNA was used by Evrogen JCS (Moscow, Russia) for ds cDNA synthesis, using the SMART approach (Zhu et al. 2001), and normalized using the DSN normalization method (Zhulidov et al. 2004). cDNA was directionally inserted into the pAL17.1 vector and transformed into E. coli with the BioRad Micropulser (Hercules, CA). cDNA clones were sequenced by SymBio Corporation (Menlo Park, CA).

text: seedling

text: E. coli

text: Pseudotsuga menziesii

text: AscI

text: NotI

text: needles, stems, and buds

text: Douglas-fir maximum cold hardiness cDNA library 2003-2004 (MH_2003-04)


characteristic: External Id

text: Oregon State University

text: live

text: Generic

text: The cDNA library was obtained from Douglas-fir seedlings in their first year of growth. All seedlings were grown outdoors in Corvallis, Oregon, USA and collected from February 2004 to April 2004. Total RNA was extracted from ~3g of ground tissue (separately from buds, stems, and needles) from a sample of 10 seedlings, following a modified protocol described in Chang et al. (1993). Total RNA was used by Evrogen JCS (Moscow, Russia) for ds cDNA synthesis, using the SMART approach (Zhu et al. 2001), and normalized using the DSN normalization method (Zhulidov et al. 2004). cDNA was directionally inserted into the pAL17.1 vector and transformed into E. coli with the BioRad Micropulser (Hercules, CA). cDNA clones were sequenced by SymBio Corporation (Menlo Park, CA).

text: seedling

text: E. coli

text: Pseudotsuga menziesii var. menziesii

text: AscI

text: NotI

text: needles, stems, and buds

text: Douglas-fir cold deacclimating cDNA library 2003-2004 (CD_2003-04)


text: Oklahoma State University

characteristic: INSDC center name

text: live

text: Generic

characteristic: cell-line

text: Total RNA was isolated from three A. marginale-infected and three uninfected IDE8 cell cultures using TriReagent (Sigma) according to manufacturer's instructions. Pools of 20 ug RNA were made from uninfected and infected tick cells. SSH was performed at Evrogen JCS (Moscow, Russia) as described previously (Naranjo et al., 2006; FEMS Immunol Med Microbiol 46: 298-312).

text: Escherichia coli

text: Ixodes scapularis

text: cDNA suppression subtraction library from Anaplasma marginale infected and uninfected Ixodes scapularis IDE8 tick cells

text: Plasmid


text: NUI

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: mid blastula

text: Danio rerio

text: Danio rerio mid blastula


text: Institute of Genetics and Cytology of Northeast Normal University

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission package

text: The total RNA was prepared with Trizol reagent from 6-year-old Panax ginseng leaf, and the full-longth cDNA expression library was constructed by SMART cDNA Library Construction Kit of CLONTECH(PT3000-1).

text: Leaf

text: Panax ginseng

text: Sfi I

text: cDNA expression library of Panax ginseng leaf

text: Phage

text: lambda TriplEx2


text: Cheju National University

text: live

text: Generic

text: Nichinan No. 1

characteristic: cultivar

text: Size in diameter of young fruit was about 10 mm

characteristic: description

text: 30 days after flowering

text: E. coli XL1-Blue

text: Citrus unshiu

text: EcoRI

text: EcoRI

text: Fruit

text: Citrus unshiu young fruit

text: Plasmid


text: Institute of Molecular Genetics

text: live

text: Generic

text: tissue and cell isolation according to Weisser et al. (2002); RNA isolation via guanidinthiocyanat method of Chomcynski and Sacchi (1987): after polyA+ purification first strand synthesis with a Not I primer-adaptor; second strand synthesis (nick translation Gubler and Hoffmann, 1983) and ligation of Sal I adaptor; Size separation of cDNA fragments and target cloning in vector pSPORT1

text: fetal

text: E. coli DH10B

text: Bos taurus

characteristic: organism

text: Not I

text: Sal I

text: growth plate

text: Bt_FGP library

characteristic: title

text: plasmid


text: Harvard University

text: live

text: Generic

text: Drosophila melanogaster

text: Drosophila melanogaster adult mRNA


text: Harvard University

text: live

text: Generic

text: Drosophila melanogaster

text: Drosophila melanogaster embryonic mRNA


text: Harvard University

text: live

text: Generic

text: Drosophila melanogaster

text: Drosophila melanogaster larval mRNA


text: Universidade Estadual de Santa Cruz (UESC)

text: live

text: Generic

text: Catongo

text: Total RNA was extracted from apical meristems from Theobroma cacao L. variety Catongo inoculated by M. perniciosa. Inoculated and non-inoculated apical meristems were harvested at 24h, 48h, 72h and then every 5 days until 90 days after inoculation. The cDNA library was constructed from pooled total RNA, using the DB SMART Creator cDNA library kit (Clontech), and the cDNA were cloned directionally into the pDNR-LIB plasmid.

text: from 24h to 90 days after Moniliophthora perniciosa infection

characteristic: dev-stage

text: Stem

characteristic: organ

text: Theobroma cacao

text: Apical meristem

text: Theobroma cacao-Moniliophthora perniciosa compatible interaction library

text: Plasmid

text: pDNR-LIB

characteristic: vector


text: live

text: Generic

text: Total RNA was extracted from apical meristems from Theobroma cacao L. variety TSH1188 inoculated by M. perniciosa. Inoculated and non-inoculated apical meristems were harvested at 24h, 48h, 72h and then every 5 days until 90 days after inoculation. The cDNA library was constructed from pooled total RNA, using the DB SMART Creator cDNA library kit (Clontech), and the cDNA were cloned directionally into the pDNR-LIB plasmid.

text: Stem

text: Theobroma cacao

text: Apical meristem

characteristic: tissue-type

text: Theobroma cacao-Moniliophthora perniciosa incompatible interaction library

text: Plasmid

text: pDNR-LIB


text: CSIRO Livestock Industries

text: live

text: Generic

text: Subtractive cDNA library made from liver vs. spleen tissue.

text: Adult

characteristic: lab-host

text: Sus scrofa

text: Rsa I

text: Rsa I

text: female

characteristic: sex

text: Large White x Landrace

characteristic: strain

text: Liver

text: Sus scrofa subtractive cDNA library from liver tissue (LIVa)

text: pGEM-Teasy


characteristic: External Id

text: CSIRO Livestock Industries

characteristic: INSDC center name

text: live

text: Generic

characteristic: NCBI submission model

text: Subtractive cDNA library made from liver vs. lymph node tissue

text: Adult

text: Sus scrofa

text: Rsa I

text: Rsa I

text: female

text: Large White x Landrace

text: Liver

text: Sus scrofa subtractive cDNA library from liver tissue (LIVb)

text: pGEM-Teasy


text: CSIRO Livestock Industries

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Subtractive cDNA library made from lymph node vs. liver tissue.

text: Adult

text: Sus scrofa

text: Rsa I

text: Rsa I

text: female

text: Large White x Landrace

text: Lymph node

text: Sus scrofa subtractive cDNA library from lymph node tissue (LN)

text: pGEM-Teasy


text: CSIRO Livestock Industries

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission package

text: Lymphocytes

characteristic: cell type

text: Subtractive cDNA library made from conA activated pig lymphocytes vs. non-activated pig lymphocytes.

text: Adult

text: Sus scrofa

text: Rsa I

text: Rsa I

text: female

text: Large White x Landrace

text: Sus scrofa subtractive cDNA library from activated lymphocytes (PLC-F)

text: pGEM-Teasy


text: CSIRO Livestock Industries

text: live

text: Generic

text: Lymphocytes

text: Subtractive cDNA library made from non-activated pig lymphocytes vs. conA activated pig lymphocytes.

characteristic: description

text: Adult

text: Sus scrofa

characteristic: organism

text: Rsa I

text: Rsa I

text: female

text: Large White x Landrace

text: Sus scrofa subtractive cDNA library from non-activated lymphocytes (PLC-R)

text: pGEM-Teasy


text: CSIRO Livestock Industries

text: live

text: Generic

text: Lymphocytes

text: Subtractive cDNA library made from conA activated pig lymphocytes vs. muscle tissue.

text: Adult

characteristic: dev-stage

text: Sus scrofa

text: Rsa I

text: Rsa I

text: female

text: Large White x Landrace

text: Sus scrofa subtractive cDNA library from activated lymphocytes vs. muscle tissue (PLMU-F)

characteristic: title

text: pGEM-Teasy


text: CSIRO Livestock Industries

text: live

text: Generic

text: Subtractive cDNA library made from muscle tissue vs. conA activated pig lymphocytes.

text: Adult

text: Sus scrofa

text: Rsa I

text: Rsa I

text: female

text: Large White x Landrace

text: Muscle

text: Sus scrofa subtractive cDNA library from muscle tissue vs. activated lymphocytes (PLMU-R)

text: pGEM-Teasy

characteristic: vector


text: CSIRO Livestock Industries

text: live

text: Generic

text: Subtractive cDNA library made from spleen vs. liver tissue.

text: Adult

text: Sus scrofa

text: Rsa I

text: Rsa I

text: female

text: Large White x Landrace

text: Spleen

characteristic: tissue-type

text: Sus scrofa subtractive cDNA library from spleen tissue (SPL)

text: pGEM-Teasy


text: CSIRO Livestock Industries

text: live

text: Generic

text: Peripheral Blood Leukocytes (PBL)

text: Subtractive cDNA library made from PBL at 24 hours after infection with Actinobacillus pleuropneumoniae (APP) vs. PBL before APP challenge

text: Adult

characteristic: lab-host

text: Sus scrofa

text: Rsa I

text: Rsa I

text: female

text: Large White x Landrace

text: Sus scrofa subtractive cDNA library from PBL 24 hours after APP challenge (WBC-F)

text: pGEM-Teasy


text: CSIRO Livestock Industries

text: live

text: Generic

text: Peripheral Blood Leukocytes (PBL)

text: Subtractive cDNA library made from PBL before Actinobacillus pleuropneumoniae (APP) infection vs. PBL at 24 hours after infection with APP

text: Adult

text: Sus scrofa

text: Rsa I

text: Rsa I

text: female

characteristic: sex

text: Large White x Landrace

text: Sus scrofa subtractive cDNA library from PBL before APP challenge (WBC-R)

text: pGEM-Teasy


text: Institut Universitaire Europeen de la Mer, Universite de Bretagne Occidentale

text: live

text: Generic

text: flounders exposed to herbicides

text: juveniles

text: Platichthys flesus

text: liver

text: subtracted libraries from flounders exposed to herbicides


text: University of California at Davis (UCD)

text: live

text: Generic

text: RACE-PCR was performed with the Marathon(TM) cDNA Amplification Kit (Clontech, Palo Alto, CA, USA) according to the manufacturer's instructions. Total RNA was extracted from the leaves of about four-week old wild-type Arabidopsis Col-0 plants using the TRIzol procedure (Invitrogen, Carlsbad, CA). Template mRNA was then purified from total RNA using Dynabeads Oligo (dT)25 (Dynal Biotech, Lake Success, NY, USA). RACE-PCR products were cloned into the pCR2.1-TOPO vector (Invitrogen, Carlsbad, CA) and sequenced.

text: E.coli

text: Arabidopsis thaliana

characteristic: strain

text: Arabidopsis thaliana Col-0 RACE (RWM_NL_2007)

text: plasmid


characteristic: External Id

text: Universita Cattolica S. Cuore

text: live

text: Generic

text: Biondo cadenera,Tarocco nucellare 57-1E-I,Moro nucellare 58-8D-I

characteristic: cultivar

text: Fruits were harvested at three developmental stages. Poly-A mRNA was isolated from total RNA extracted from flesh according to the Trizol LS and Dynabeads M-280 Streptavidin protocols. A cDNA-AFLP technique of RNA fingerprinting was optimized allowing the detection of DNA fragments derived from RNA using cDNA synthesis and subsequent polymerase chain reaction (PCR) amplification. This is based on the selective amplification of cDNA fragments obtained by digestion with one frequent and one rare cutter enzymes, followed by ligation of two double-stranded adapters of known sequences. AFLP-TP (Transcriptional Profiling) is an improved version of the cDNA-AFLP protocol, that allows the isolation one unique restriction fragment for each cDNA. The 'esacutter' enzyme is BstYI (recognition site Pu/GATCPy) and the 'fourcutter' is MseI (recognition site T/TAA). The difference in AFLP-TP lies in an additional step between the two digestions: as the cDNA synthesis is obtained with a biotinylated oligo-dT, this allows to collect the 3' end of the transcript after the first digestion, taking advantage of the high affinity of the biotin for the streptavidin anchored to magnetic beads (Dynal). After the electrophoretic run, a vertical scanner collected the fluorescence emissions derived from the excitation of the fluorescein molecules attached to the BstYI primers with a 465nm monochromatic beam. Single fragments were isolated either through DGGE (Denaturing Gradient Gel Electrophoresis) analysis or cloning into pGEM-T Easy vector (Promega). Samples that showed only one neat band were sequenced.

text: three developmental stages during ripening period

text: Citrus sinensis

text: BstYI

text: MseI

text: flesh

text: Citrus sinensis flesh cDNA-AFLP Library

text: Plasmid

text: PGEM Teasy vector


text: NRC Institute for Marine Biosciences Industrial Partnership Facility

characteristic: INSDC center name

text: live

text: Generic

characteristic: NCBI submission model

text: Gadus morhua

text: brain

text: Gadus morhua brain thermal stress library gmnbbrts


text: University of Agricultural Sciences

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: 15 days old Mulberry seedlings were subjected to different levels of moisture stress (70%FC, 50%FC and 100%FC) by gradually withholding water till the plants withstand the respective stress level. RNA was isolated from the leaf tissues and subtracted cDNA library was constructed using T/A cloning kit.

text: Developmental stage (15 days old seedlings)

text: E. coli

text: Morus indica

text: Eco RI

text: Leaf

text: Mulberry S-13 dehydration stress subtracted cDNA library

text: Plasmid (TA cloning vector)


text: Instituto Nacional de Investigaciones Forestales, Agricolas y Pecuarias, Campo Experimental Bajio

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission package

text: Tissue culture cells

text: TIANSJ98, TADH-XO

characteristic: cell-line

text: Suppression subtractive hybridization library was prepared using 0 and 21% PEG stressed cells mRNA. Abundant cDNAs were cloned in 'T' vector PCR-TOPOII and or blunt ended pMosBlue.

text: Bouteloua gracilis

text: NsiI

text: HindIII

text: Osmotic-stressed Bouteloua gracilis cultured cells Library

text: plasmid

text: pCRTOPOII and or pMOSblue


text: SCRI

text: live

text: Generic

text: Aphid cDNA library constructed from mRNA purified from mixed stage asexual females feeding on Brassica napus.

characteristic: description

text: mixed stages

text: Myzus persicae

characteristic: organism

text: asexual female

text: 2001-12 (red)

text: whole body

text: Myzus persicae 2001-12 (red), Fenton

text: plasmid


text: University of Nevada

text: live

text: Generic

text: Messenger RNA purified from PRRSV-infected MARC-145 cells at 72 hours post infection was used in the construction of 5' unidirectional EcoRI/XhoI cDNA library in the lambda ZAPII vector. Inserts in the library were excised and rescued in XL1-Blue MR F' using the ExAssist helper phage in-vivo excision system then transformed in E.coli SOLR strain. Resultant bacterial colonies were picked randomly, plasmid DNA prepared and single-pass sequenced from the 5' end using the SK+ primer.

text: E. coli SOLR

text: Sus scrofa

text: EcoRI

text: XhoI

text: MARC-145 cell line - PRRSV-exposed

characteristic: title

text: Lambda ZAPII


text: University of Nevada

text: live

text: Generic

text: Macrophage

characteristic: cell type

text: Messenger RNA purified from the alveolar macrophages obtained from experimentally PRRSV-infected pig at day 75 post infection was used in the construction of 5' unidirectional EcoRI/XhoI cDNA library in the lambda ZAPII vector. Inserts in the library were excised and rescued in XL1-Blue MRF' using the ExAssist helper phage in-vivo excision system then transformed in E.coli SOLR strain. Resultant bacterial colonies were picked randomly, plasmid DNA prepared and single-pass sequenced from the 5' end using the SK+ primer.

text: Lung

characteristic: organ

text: Sus scrofa

text: EcoRI

text: XhoI

text: Male

text: Alveolar macrophage from PRRSV-infected pig

text: Lambda ZAPII

characteristic: vector


text: University of Nevada

text: live

text: Generic

text: Epithelial

text: PK13 (CRL6489)

text: Messenger RNA purified from PRRSV-exposed porcine kidney epithelial cell line PK13 (CRL6489) at 72 hours post viral exposure was used in the construction of 5' unidirectional EcoRI/XhoI cDNA library in the lambda ZAPII vector. Inserts in the library were excised and rescued in XL1-Blue MR F' using the ExAssist helper phage in-vivo excision system then transformed in E.coli SOLR strain. Resultant bacterial colonies were picked randomly, plasmid DNA prepared and single-pass sequenced from the 5' end using the SK+ primer.

text: E. coli SOLR

characteristic: lab-host

text: Sus scrofa

text: EcoRI

text: XhoI

text: Porcine kidney epithelial cell line [PK13; CRL6489] - PRRSV-exposed

text: Lambda ZAPII


text: University of Nevada

text: live

text: Generic

text: Messenger RNA purified from the spleen tissue obtained from experimentally PRRSV-infected pig at day 75 post infection was used in the construction of 5' unidirectional EcoRI/XhoI cDNA library in the lambda ZAPII vector. Inserts in the library were excised and rescued in XL1-Blue MRF' using the ExAssist helper phage in-vivo excision system then transformed in E.coli SOLR strain. Resultant bacterial colonies were picked randomly, plasmid DNA prepared and single-pass sequenced from the 5' end using the SK+ primer.

text: E. coli SOLR

text: Spleen

text: Sus scrofa

text: EcoRI

text: XhoI

text: Male

characteristic: sex

text: Spleen from PRRSV-infected pig

text: Lambda ZAPII


characteristic: External Id

text: University of Nevada

text: live

text: Generic

text: Messenger RNA purified from the testicular tissue obtained from experimentally PRRSV-infected pig at day 75 post infection was used in the construction of 5' unidirectional EcoRI/XhoI cDNA library in the lambda ZAPII vector. Inserts in the library were excised and rescued in XL1-Blue MRF' using the ExAssist helper phage in-vivo excision system then transformed in E.coli SOLR strain. Resultant bacterial colonies were picked randomly, plasmid DNA prepared and single-pass sequenced from the 5' end using the SK+ primer.

text: E. coli SOLR

text: Testis

text: Sus scrofa

text: EcoRI

text: XhoI

text: Male

text: Porcine Testis from PRRSV-infected pig

text: Lambda ZAPII


text: University of Delhi South Campus

text: live

text: Generic

characteristic: cultivar

text: The cDNA library was constructed from polyA+ RNA by using Universal PCR amplification method. The cDNAs were cloned randomly in pGEM-T Easy vector.

text: Mature

characteristic: dev-stage

text: Morus indica

text: EcoRI

text: EcoRI

text: hermaphrodite

text: Leaf

characteristic: tissue-type

text: Expressed sequence tags from cDNA library of mature leaf of mulberry

text: Plasmid

text: pGEM-T Easy


text: Yunnan Tobacco Science Research Institute

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

characteristic: NCBI submission model

text: two months

text: Nicotiana tabacum

text: leaf

text: Salicylic acid induced SSH library in tobacco

text: T-easy vector


text: Crop and Food Research

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission package

text: Mixed

text: Total RNA was isolated from unopened immature flower buds of onion from multiple populations, including plants in both N and S cytoplasm, and msms (maintainer) and Ms_ at the nuclear restorer locus. Non-normalised cDNA library was synthesized by Invitrogen USA.

text: Unopened, immature

text: Flowers

text: Allium cepa

text: EcoRV

text: mixed

text: Onion immature floral cDNA library

text: Phagemid

text: pCMV.SPORT 6.1


text: Jiangsu Academy of Agricultural Sciences (JAAS)

text: live

text: Generic

text: Wheat spikes were inoculated with F. graminearum spores at heading stage. Inoculated spikes were harvested 8h, 12h, 24h, 36h, 48h after inoculation. Total RNA and mRNA were extracted by Trizol(Tiangen) and Oligotex mRNA mini kit(Qiagen), respectively. The SSH-cDNA library was conducted by using PCR Select cDNA Subtraction Kit(Clontech company). Poly (A+) RNA from mixed spikes of Sumai3 as tester, mRNA from mixed spikes of Sumai3's susceptible near-isogenic lines as driver. The enriched SSH PCR products were purified and cloned into pMD-18 T vector and transformed to E.coli strain Top10.

characteristic: description

text: heading stage

text: Triticum aestivum

characteristic: organism

characteristic: strain

text: F. graminearum inoculated spike

text: SSH-cDNA Library during wheat-Fusarium graminearum interaction in spikes of wheat


text: College of Agronomy, Shandong Agricultural University

text: live

text: Generic

text: Chinese Spring

text: method of preparation: cold acclimation.

text: seedling

text: leaf

characteristic: organ

text: Triticum aestivum

text: leaf

text: Wheat seedling stage cDNA library

characteristic: title

text: pGEM-T


text: University of Fukui

text: live

text: Generic

text: we obtained these sequences by differential display PCR analysis

text: liver

text: Mus musculus

text: Mus musculus C57BL/6 liver differential display PCR


text: Tianjin Medical University General Hospital

text: live

text: Generic

text: Large cell lung cancer

characteristic: cell type

characteristic: cell-line

text: Homo sapiens

text: Lung

text: Human large cell lung cancer cell line subtracted cDNA library


characteristic: External Id

text: University of California, Riverside

text: live

text: Generic

text: mix (Phytophthora and Potato tuber)

text: subtracted against potato RNA by SSH-PCR

text: 97-368 (US-8 genotype)

characteristic: isolate

text: Phytophthora infestans

text: PiTA - Interaction library between Potato tuber and Phytophthora infestans

text: pSPORT I

characteristic: vector


text: University of California, Riverside

text: live

text: Generic

text: mix (Phytophthora and Potato tuber)

text: 97-368 (US-8 genotype)

text: Phytophthora infestans

text: PiTC - Interaction library between Potato tuber and Phytophthora infestans

text: pSPORT I


text: University of California, Riverside

text: live

text: Generic

text: mix (Phytophthora and Potato tuber)

text: 97-368 (US-8 genotype)

text: Phytophthora infestans

text: PiTE - Interaction library between Potato tuber and Phytophthora infestans

text: pSPORT I


text: University of California, Riverside

text: live

text: Generic

text: mix (Phytophthora and Potato tuber)

text: 97-368 (US-8 genotype)

text: Phytophthora infestans

text: PiTB - Interaction library between Potato tuber and Phytophthora infestans

text: pSPORT I


text: Northwest A&F University

text: live

text: Generic

text: young leaves

text: Xiaoyan 22

characteristic: cultivar

text: Total RNA was extracted by Trizol, reverse transcription was conducted by A-MLV reverse transcriptase; then double strand cDNA was synthesized and subjected to SSH analysis by using PCR Select cDNA Subtraction Kit (Clontech) after twice hybridization. The PCR products were purified and cloned into pGEM-T Easy vector and transformed to E coli strain DH5a, sequencing were conducted by Sp6 or T7 primer at LiCOR 4200 DNA Sequencer.

text: stage of 4-5 leaves

characteristic: dev-stage

text: E. coli DH5a

characteristic: lab-host

text: Triticum aestivum

text: leaves

characteristic: tissue-type

text: SSH-cDNA Library after serious drought in leaves of wheat


text: Northwest A&F University

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

characteristic: NCBI submission model

text: young leaves

text: Shaan 229

text: stage of 4-5 leaves

text: E. coli DH5a

text: Triticum aestivum

text: leaves

text: SSH-cDNA library of wheat leaves during rehydration after serious water stress


text: AT Bioscience

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission package

text: Homo sapiens

characteristic: organism

text: renal carcinoma

text: Homo sapiens renal carcinoma


text: University of Arizona

text: live

text: Generic

text: Gene specific primers used to amplify gene fragments from 1st strand cDNA

characteristic: description

text: 7 to 10 day old

text: Arabidopsis thaliana

text: Ecotype Columbia

characteristic: strain

text: Seedlings

text: Direct sequencing of RT-PCR products of Arabidopsis thaliana

characteristic: title


characteristic: External Id

text: Nagasaki University

text: live

text: Generic

text: Rotifers were harvested by filtration onto a plankton net (45 micron mesh) that was washed with sterilized artificial seawater; rotifers were resuspended in 400 ml seawater that was exchanged every 2-3 hours over 12 hours to allow the rotifers to consume any remaining Chlorella and excrete their gut contents. The washed and starved rotifers were collected by plankton net and suspended in ISOGEN (Nippon Gene, Japan). The rotifers were homogenized with 0.8 mm glass beads using a vortex mixer and total RNA was isolated from the homogenate according to the manufacturer.s instructions. Poly(A)+ RNA was purified from total RNA using MagExtractor (Toyobo, Japan). About 2.8 ug of PolyA RNA was used in first strand synthesis using a cDNA Synthesis Kit (Amersham Bioscience, USA). The blunt-ended cDNA was ligated to the EcoRI/NotI adaptor, then ligated into EcoRI-predigested lambda-ZAPII arms (Stratagene, USA), and packaged in vitro by using Gigapack III gold packaging extract (Stratagene).

text: All stages represented

text: Brachionus plicatilis

text: EcoRI

text: EcoRI

text: Population of mictic and amictic females and males

characteristic: sex

text: All tissues, including resting eggs

text: Brachionus plicatilis NHIL unnormalized cDNA library

text: Plasmid

text: pBluescript SK(-)


text: Station Biologique de Roscoff

text: live

text: Generic

text: scallop gonad

text: adult

text: Argopecten purpuratus

text: male, female and immature individuals

text: testis, ovary and immature gonad

text: Chilean scallop gonad Library


text: Centro de Investigacion en Alimentacion y Desarrollo

text: live

text: Generic

text: white spot syndrome virus infected gills library

text: Penaeus vannamei

text: gills

text: Litopenaeus vannamei white spot syndrome virus infected gills


text: Fujian Agriculture and Forestry University

text: live

text: Generic

text: Minhua 6

text: The full length cDNA library was constructed from peanut cultivar Minhua 6. The peanut pod from DAP 9 to 50 were collected, and placed into liquid N2 immediately and stored in -80oC freezer. Total RNA was isolated TRNZOL-Reagent (Tangen) and purified and mRNA was extracted from total RNA by mRNA extration kit (promega). cDNA synthesis by primer extension and library construction followed the protocol of Creator SMART cDNA Library Construction Kit. The cDNA above 500bp were collected after size-fraction. The inserts were directionally cloned into pDNR-LIB vector using the asymmetrical Sfi I sites, Sfi1a and Sfi1b sites. The library was constructed by Dr. Ninbo Cai and Dr. Weijian Zhuang.

text: 9 to 50 days after pegging

text: Pods

characteristic: organ

text: Arachis hypogaea

text: Full length peanut pod cDNA library

text: plasmid

text: pDNR-LIB

characteristic: vector


text: Kansas State University

text: live

text: Generic

text: Library constructed by Yoonseong Park

text: Adult younger than 1-month post eclosion

characteristic: dev-stage

text: Tribolium castaneum

text: NotI

text: MluI

text: Georgia 2

text: Hindgut and Malpighian tubules

text: plasmid


text: Biomedical Research Institute

text: live

text: Generic

text: Poly(A)+ RNA was isolated from the hepatopancreas of unexposed Biomphalaria glabrata (BS-90) snails and first strand cDNA synthesized using an oligo-dT primer-linker (XhoI). Second strand synthesis was followed by the ligation of EcoRI adaptors. Following digestion with XhoI and EcoRI, the completed, directional cDNA was cloned into Uni-ZAP XR Phagemid vector obtained from Stratgene. Mass excision of the unamplified libraries was performed and recombinant phagemids were stored in 96-well format ordered arrays.

text: Biomphalaria glabrata

text: EcoRI

text: XhoI

text: hermaphrodite

text: Biomphalaria glabrata resistant snail (BS-90) hepatopancreas library

text: phagemid

text: pBluescript SK-


text: Pennsylvania State University

text: live

text: Generic

text: Library for RNA-ligase mediated 5'-RACE. polyA+ RNA was first reacted with T4 RNA ligase and an RNA adapter without CIP pre-treatment, and thus was enriched in uncapped mRNAs. The pool was then reverse transcribed with an oligodT primer to make 1st strand cDNA. Various gene-specific primers were then used to find truncated cDNAs corresponding to the 3' fragments left by microRNA-mediated mRNA cleavage.

text: Physcomitrium patens

text: Protonemata and young leafy gametophores

text: 17 day-old wild-type P. patens


text: Beijing Genomics Institute, Chinese Academy of Sciences

text: live

text: Generic

text: Adult (20 days after hatching begining)

text: E. coli (DH10B)

characteristic: lab-host

text: Artemia franciscana

text: EcoRI

text: XhoI

text: mixed

text: Whole body

text: Brine Shrimp Adult (20 days after hatching begining)

text: pBluescript II XR vector (Stratagene)


text: live

text: Generic

text: Diapaused cysts

text: E. coli (DH10B)

text: Artemia franciscana

text: EcoRI

text: XhoI

text: mixed

text: embryos (cysts)

characteristic: tissue-type

text: Brine Shrimp diapaused embryos (cysts)

text: pBluescript II XR vector (Stratagene)


characteristic: INSDC center name

text: live

text: Generic

text: embryos, 5 hours after hatching begining

text: E. coli (DH10B)

text: Artemia franciscana

text: EcoRI

text: XhoI

text: mixed

text: embryos (cysts)

text: Brine Shrimp embryos, 5 hours after hatching

text: pBluescript II XR vector (Stratagene)


characteristic: INSDC first public

text: live

text: Generic

text: embryos, 10 hours after hatching

text: E. coli (DH10B)

text: Artemia franciscana

text: EcoRI

text: XhoI

text: mixed

text: embryos (cysts)

text: Brine Shrimp embryos, 10 hours after hatching

text: pBluescript II XR vector (Stratagene)


characteristic: INSDC last update

text: live

text: Generic

text: embryos, 15 hours after hatching

text: E. coli (DH10B)

text: Artemia franciscana

text: EcoRI

text: XhoI

text: mixed

text: embryos (cysts)

text: Brine Shrimp embryos, 15 hours after hatching

text: pBluescript II XR vector (Stratagene)


text: Dept. Biology and C.R.I.B.I.-Biotechnology Centre, University of Padova

text: live

text: Generic

characteristic: NCBI submission model

text: Total RNA extracted from head (pool of 5 samples) was used to to make 3'-end cDNA library using a combination of SMART protocol (Clontech), ensuring almost full-length cDNA, and Gateway technology (Invitrogen), allowing unidirectional cloning without enzymatic digestion.

text: E.coli DH10B

text: Head

text: Euphausia superba

text: pool of males and females

text: retina, brain and midgut

text: pDONR221 (Invitrogen)


text: live

characteristic: INSDC status

text: Generic

text: Total RNA extracted from body (pool of 5 samples) was used to to make 3'-end cDNA library using a combination of SMART protocol (Clontech), ensuring almost full-length cDNA, and Gateway technology (Invitrogen), allowing unidirectional cloning without enzymatic digestion.

text: E.coli DH10B

text: Body

text: Euphausia superba

text: pool of males and females

text: pDONR221 (Invitrogen)


text: live

text: Generic

characteristic: NCBI submission package

text: Total RNA extracted from legs (pool of 5 samples) was used to to make 3'-end cDNA library using a combination of SMART protocol (Clontech), ensuring almost full-length cDNA, and Gateway technology (Invitrogen), allowing unidirectional cloning without enzymatic digestion.

text: E.coli DH10B

text: Legs

text: Euphausia superba

text: pool of males and females

text: pDONR221 (Invitrogen)


text: National Institute for Plant Genome Research

text: live

text: Generic

characteristic: cultivar

text: cDNA library cloned into TOPO plasmid vector

text: Cicer arietinum

characteristic: organism

text: immature seed

text: chickpea immature seed library

text: plasmid

text: TOPO


text: Kansas State University

text: live

text: Generic

text: Normalized cDNA library

characteristic: description

text: Adult younger than 1-month post eclosion

text: Tribolium castaneum

text: Georgia 2

characteristic: strain

text: Head

text: Plasmid


characteristic: External Id

text: Kansas State University

text: live

text: Generic

text: Normalized cDNA library, Full-length selected by Invitrogen, recombination based cloning

text: Late larva

text: Tribolium castaneum

text: Georgia 2

text: Carcass after removal of alimentary cannel, Immune challenged

characteristic: title

text: Plasmid


text: Kansas State University

text: live

text: Generic

text: TL2 is the normalized TL1 by Invitrogen

text: Mixed larval stages

text: Tribolium castaneum

text: Georgia 2

text: Whole body

text: Plasmid


text: Kansas State University

text: live

text: Generic

text: Normalized cDNA library

text: Tribolium castaneum

text: Female

characteristic: sex

text: Georgia 2

text: Ovary

text: Plasmid


text: University of Queensland

text: live

text: Generic

text: Uncultured microbial mRNA clone

text: uncultured microorganism

text: Aquatic microbial community

text: Plasmid

text: pCR-Blunt

characteristic: vector


text: University of Queensland

text: live

text: Generic

text: Uncultured microbial mRNA clone

text: uncultured microorganism

text: Bovine rumen microbial community

text: Plasmid

text: pCR-Blunt


text: University of Queensland

text: live

text: Generic

text: Uncultured microbial mRNA clone

text: uncultured microorganism

text: Human intestinal microbial community

text: Plasmid

text: pCR-Blunt


text: University of Queensland

text: live

text: Generic

text: Uncultured microbial mRNA clone

text: uncultured microorganism

text: Human oral microbial community

text: Plasmid

text: pCR-Blunt


text: University of Queensland

text: live

text: Generic

text: Uncultured microbial mRNA clone

text: uncultured microorganism

text: Terrestrial soil microbial community

text: Plasmid

text: pCR-Blunt


text: NORTHEAST FORESTRY UNIVERSITY

text: live

text: Generic

text: Fruit ripening stage

characteristic: dev-stage

text: Panax ginseng

text: leaves

text: cDNA library of red fruit Panax ginseng leaves at fruit ripening stage

text: pDNR-LIB


text: Northwest Fisheries Science Center

text: live

text: Generic

text: 2-year old fish with cortical alveolus stage oocytes

text: ovary

characteristic: organ

text: Oncorhynchus kisutch

text: female

text: follicle/interstitial cell enriched ovarian tissue

text: Coho salmon ZAP Express ovarian library

text: Phage

text: pBK-CMV


text: Medical University of South Carolina

text: live

text: Generic

text: Skin

characteristic: cell type

text: Eubalaena glacialis

text: Skin

characteristic: tissue-type

text: Plasmid


text: Medical University of South Carolina

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: Skin

text: Eubalaena glacialis

text: Skin

text: RightWhaleSkin

text: Plasmid


text: The University of Iowa

characteristic: INSDC last update

text: live

text: Generic

characteristic: NCBI submission model

text: Goniomonas cf. pacifica

text: XhoI

text: EcoRI

text: CCMP 1869

text: Bacterial

text: pBluescript II SK (+) Stratagene


text: Faculdade de Medicina de Ribeirio Preto da Universidade de Sao Paulo

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission package

text: DDRT-PCR library was performed in accordance with Liang and Pardee, 1992. This library was created to identify T.rubrum differentialy expressed genes.

text: E.coli MOS Blue

characteristic: lab-host

text: Trichophyton rubrum

text: Trichophyton rubrum DDRT-PCR cDNA Library

text: Plasmid

text: pGEM-T


text: live

text: Generic

text: Forward SSH cDNA library of Ascochyta rabiei induced Cicer arietinum genes, cloned in pDRIVE U/A PCR cloning vector. Single-pass sequencing performed using M13 Forward primer. The library tags are not present the submitted sequences.

characteristic: description

text: Cicer arietinum

characteristic: organism

text: Ascochyta rabiei induced library

text: Plasmid

text: pDRIVE Cloning vector (Qiagen)


characteristic: External Id

text: Kunming Medical College

text: live

text: Generic

text: bronchial epithelial cells

text: Immortalized human bronchial epithelial cells(BEAS-2B)

characteristic: cell-line

text: Suppression subtractive hybridization(SSH), T/A clone, dot blot, DNA squencing.

text: E.coli DH5a

text: Homo sapiens

text: EcoR I

text: EcoR I

text: A cDNA subtractive library of malignant transformation cells induced by mineral powder in GeJiu

text: Plasmid

text: pGM-T


text: University of Texas at Austin

text: live

text: Generic

text: Vegetative

text: mRNA was isolated from late log-phase axenic cell cultures collected at various times during the light and dark phases of the photoperiod, which was later pooled. Directional cloning of cDNA positioned the 5' end of the insert at the EcoRI site; the poly-A(+) tail is located at the XhoI site of the plasmid. Part or all of the EcoRI linker sequence (GGCACGAGG) may be present at the 5' end of the sequence.

text: E. coli

text: Prymnesium parvum

text: EcoRI (5' end)

text: XhoI (3' end)

text: Asexual

characteristic: sex

text: UTEX 2797 (Texas isolate)

characteristic: strain

text: Haptophyte (alga)

text: PPcD

characteristic: title

text: plasmid

text: pBluescript II XR (Stratagene)


text: Tropical Medicine Institute,Guangzhou University of Traditional Chinese Medicine

text: live

text: Generic

text: Artemisia annua

text: leaf

text: Artemisia annua leaf cDNA library


text: Lund University

text: live

text: Generic

text: The insects were kept at 23 C, 70 percent humidity and a 18:6 h Light:Dark cycle. Total RNA was extracted from ovipositors including pheromone glands from 10 females 0-1 day post emergence, 1.5-2 h into the scotophase. The cDNA library was constructed using the SMART cDNA Construction Kit (BD Biosciences) according to the manufacturers' protocol. Full length cDNAs were directionally ligated into (lambda) TriplEx2 arms after trimming by SfiI. The lambda library was converted into a plasmid library in a Cre+ b/w strain (E. coli BNN132). Plasmid clones were collected randomly and used as template for PCR amplification of inserts using the plasmid-specific primers P104 and P105, followed by DNA sequencing using the forward primer P104.

text: Adult 0-1 day post emergence

characteristic: dev-stage

text: Escherichia coli BNN132

text: Agrotis segetum

text: SfiI

text: SfiI

text: Female

text: As12, Biologische Bundesanstalt fur Land- und Fortwirtschaft, Darmstadt, Germany

text: Pheromone gland

text: As12G - Agrotis segetum pheromone gland library

characteristic: vector


text: Riken

text: live

text: Generic

text: Mus musculus

text: retina

characteristic: tissue-type

text: Mus musculus retina E13


text: The Institute for Genomic Research (TIGR; www.tigr.org)

characteristic: INSDC center name

text: live

text: Generic

text: Globisporangium ultimum DAOM BR144

text: DAOM BR144 (=CBS 805.95 =ATCC 200006)

text: Pythium ultimum ESTs


text: St Jude Children's Research Hospital

characteristic: INSDC first public

text: live

text: Generic

text: oligo dT primed cDNA library using total RNA from whole Trichoplax adhaerens. Size-sorted double-stranded cDNA was amplified by linker-specific primers and TOPO cloned into pcDNA3.

text: Trichoplax adhaerens

text: XbaI

text: MscI

text: whole animal

text: Trichoplax adhaerens cDNA library

text: plasmid


text: College of Resources and Environment, South China Agricultural University

characteristic: INSDC last update

text: live

text: Generic

text: total RNA extracted from mixed stages of Radopholus similis inoculated within Musa paraolisiaca seedling for 3d

text: Radopholus similis


text: Institute of Ramie, Hunan Agriculture University

text: live

text: Generic

characteristic: NCBI submission model

text: Zhongzhu 1

characteristic: cultivar

text: Middle-growing stage

text: E.Coli DH10B

text: Boehmeria nivea

text: EcoRI

text: NotI

text: Bark

text: Bark cDNA library of ramie (Boehmeria nivea (L.) Gaud) cv. Zhongzhu 1

text: Plasmid


text: Fundacion Ciencia para La Vida

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission package

text: whole plants subjected by 30 min at 4 celsius degrees

text: leaves

characteristic: organ

text: Eucalyptus globulus

text: Xho 1

text: leaves

text: Eucalyptus globulus under low temperature

text: pExpress 1


text: live

text: Generic

text: Noy Yizre'el

text: Partial sequences, cloned based on homology to conserved sequences. More information is available at http://melon.bti.cornell.edu

characteristic: description

text: Cucumis melo

characteristic: organism

text: 5' EcoRI

text: 3' XhoI

text: fruit

text: Melon carotenoid/organic acid metabolism related gene library

text: Plasmid

text: pGEM-T easy


characteristic: External Id

text: Auburn University

text: live

text: Generic

text: 1 year old plants

text: Citrus trifoliata

text: leaves

text: Poncirus trifoliata leaves 1 year old plants


text: University of Padova

text: live

text: Generic

text: Golden Delicious

text: Malus domestica

text: Cortex

text: Malus domestica cortex cDNA-AFLP differential display

text: Plasmid

text: pGem-T Easy


text: Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais

text: live

text: Generic

text: sequences obtained by differential display approach

text: adult

text: Canis familiaris

characteristic: lab-host

text: Ancylostoma braziliense

text: Ancylostoma braziliense differentially expressed mRNA


text: Huazhong Agricultural University

text: live

text: Generic

text: Suppression subtractive hybridizations between female and male chicken embryonic gonads were performed using the PCR-selected cDNA Subtraction kit (Clontech).

text: embryonic day 3.5-6 post incubation at every half an day

text: E. coli DH5a

text: Gallus gallus

text: female

characteristic: sex

text: White Leghorn

characteristic: strain

text: gonad and mesonephros from embryos larger than 5th day, but including other adjacent tissue from 3.5-4.5th day embryos

text: female chicken embryonic gonads subtracted library

text: pGEM-T


text: Huazhong Agricultural University

text: live

text: Generic

characteristic: dev-stage

text: E. coli DH5a

text: Gallus gallus

text: male

text: White Leghorn

text: male chicken embryonic gonads subtracted library

characteristic: title

text: pGEM-T


text: University of Warwick

text: live

text: Generic

text: Sclerotinia sclerotiorum

text: Paraphaeosphaeria minitans

text: Coniothyrium minitans from sclerotia of host


text: University of Constance

text: live

text: Generic

text: yeast signal sequence trap method

text: haustorium (HST), appressorium (ISSP)

text: Uromyces viciae-fabae

text: Stage specific secretomes of Uromyces fabae

characteristic: vector


text: University of Illinois at Chicago

characteristic: INSDC center name

text: live

text: Generic

text: Pretransformed cDNA library in Yeast strain AH109. Cloning method was homologous recombination.

text: brain

text: Homo sapiens

text: Matchmaker hBrain in Yeast, Clontech (Cat.#638866)


text: Clemson University

characteristic: INSDC first public

text: live

text: Generic

text: E. coli

text: Musca domestica

text: EcoRV

text: MDLTEa

text: Plasmid

text: pBluescript


text: China Agricultural University

characteristic: INSDC last update

text: live

text: Generic

text: 4 month old (adult)

text: XL1-Blue MRF1 and SOLRTM

text: Sus scrofa

text: large white

text: adipose, back fat

characteristic: tissue-type

text: porcine adipose, back fat cDNA library

text: Uni-ZAPTM and pBluescript SK(+)


text: Indain Institute of Integrative Medicine

text: live

text: Generic

characteristic: NCBI submission model

characteristic: cultivar

text: Total RNA extracted from the leaf tissue and subsequently constructed into cDNA library. Clones were screened and the amplified products were sequenced using M-13 primer.

text: matured plant

text: Withania somnifera

text: Bisexual

text: Leaf

text: Withania somnifera leaf tissue cDNA

text: UNI-ZAP expression vector


text: University of Minnesota

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission package

text: Macoun

text: suppression subtractive hybridization technique was utilized to compare mRNA populations from fresh and three-month stored Macoun apple fruit tissue

text: Postharvest

text: E. Coli

text: Fruit

text: Malus pumila

text: Mesocarp

text: Malus pumila Differential Display library

text: Plasmid

text: pST Blue1


text: The Samuel Roberts Noble Foundation

text: live

text: Generic

text: Medicago truncatula A17 plants, were grown in pots containing 75% Metro Mix 350 soil and 25% sand. Glands were dislodged from tissues by vigorously vortexing tissues in an isotonic buffer. Glands were harvested by sieving out larger tissues and by differential centrifugation. RNA for library construction was isolated from pure gland preparations using the Qiagen RNA Easy kit. The Creator SMART cDNA Library Construction Kit from Clontech was used for library construction.

characteristic: description

text: Glands were isolated from young pre-flowering stems and leaves.

text: Medicago truncatula

text: Sfi I A (GGCCATTACGGCC)

text: Sfi I B (GGCCGCCTCGGCC)

text: Isolated glandular trichomes

text: Medicago truncatula A17 glandular trichome

text: pDNR_lib (Clontech)


text: University of Rochester

text: live

text: Generic

text: pupa and adult

text: Sarcophaga bullata

text: Nasonia giraulti

characteristic: organism

text: EcoR I

text: Xho I

text: NGPA

text: pBluescript II SK(+) phagemid


characteristic: External Id

text: University of Rochester

text: live

text: Generic

text: larva

text: Sarcophaga bullata

text: Nasonia giraulti

text: EcoR I

text: Xho I

text: NGPP

text: pBluescript II SK(+) phagemid


text: University of Rochester

text: live

text: Generic

text: pupa and adult

text: Sarcophaga bullata

characteristic: lab-host

text: Nasonia vitripennis

text: EcoR I

text: Xho I

text: AsymCX x Lbii

text: NVPA

text: pBluescript II SK(+) phagemid


text: University of Rochester

text: live

text: Generic

text: larva

text: Sarcophaga bullata

text: Nasonia vitripennis

text: EcoR I

text: Xho I

text: AsymCX x Lbii

text: NVPP

text: pBluescript II SK(+) phagemid


text: INRA

text: live

text: Generic

text: ecotype: Sierra de Guadarrama, Spain; The library was made from buds of adult trees. A mixture of genotypes was used. Oligo-dT primed cDNA was directionally cloned into the EcoRI-XhoI lambda-ZAP vector arms and mass-excised to form a pBluescript phagemid.

text: adult trees

text: Pinus pinaster

text: Pinus pinaster developing buds collected on several trees adult trees

text: Uni-ZAP XR


text: Forestry and Forest Products Research Institute

text: live

text: Generic

text: Cryptomeria japonica

text: needle

text: Cryptomeria japonica needle


text: live

text: Generic

text: Cryptomeria japonica

text: pollen

text: Cryptomeria japonica pollen (Ujino-Ihara,T.)


text: Auburn University

text: live

text: Generic

text: Whole body

characteristic: organ

text: Musca domestica

text: EcoR I

text: EcoR I

text: House fly subtractive cDNA library

text: Plasmid

text: PCRTm 2.1 TA vector


text: The Samuel Roberts Noble Foundation

text: live

text: Generic

text: The mid-developmental stage female flower (cone) of Hops (Humulus lupulus) plants, cultivar Phoenix, were collected form plants grown at the Hop Research Institite, Wye, Kent, UK. The cones were stripped off the branches, and the largest cones (which were unlikely to develop much further), and the smaller cones (which contain few trichomes) were discarded. Total RNA isolated from hops trichome prepared by shaking the cones in a 50 mL tubes by using Cold-Phenol protocol. The cDNA library from hops trichome was constructed using the Creator Smart cDNA library construction kit (Clontech, Palo Alto, CA, USA) following the manufacturer's protocol for total RNA.

text: Humulus lupulus

text: Sfi IA (GGCCATTACGGCC)

text: Sfi IB (GGCCGCCTCGGCC)

text: Trichome

text: Humulus lupulus (L. cultivar Phoenix) Trichome isolated from the female flower at mid-developmental stage

text: pDNR-LIB


text: Department of Botany, University of Delhi

text: live

text: Generic

text: Xanthi

text: Suppression Subtractive Hybridization between early stage and late stage of anther development

text: Early stage of anther development

text: E.coli

text: Anther

text: Nicotiana tabacum

text: Hermaphrodite

characteristic: sex

text: Anther

text: Early stage of anther development specific suppression subtractive cDNA library

text: Plasmid

text: pGEMT Easy


text: Carnegie Institution of Washington

text: live

text: Generic

text: Clones of Geranium dissectum cDNA were probed with appropriate primers for the genes of interest and the products sequenced.

text: Jasper Ridge Biological Preserve, Stanford, CA

characteristic: isolate

text: Leaf

text: Geranium dissectum

text: Leaf

text: Geranium dissectum Jasper Ridge Biological Preserve leaves

text: pCR-XL-TOPO


text: University of Rochester

text: live

text: Generic

text: Adult

characteristic: dev-stage

text: Drosophila ananassae

text: Tuscon Drosophila stock center number 14024-0371.13

characteristic: strain

text: Whole fly

text: Drosophila ananassae endosymbiont inserts

characteristic: title


text: Queensland Institute of Medical Research

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: cDNA reverse transcribed from serum-stimulated and non-stimulated control A. caninum L3 was subjected to suppression subtractive hybridization (SSH) in order to produce libraries enriched for differentially expressed mRNAs. The forward subtracted library contains cDNAs enriched for mRNAs up-regulated upon serum stimulation while the reverse subtracted library is comprised of cDNAs enriched for mRNAs that are down-regulated upon serum stimluation. Following subtractive hybridization, cDNAs were subjected to two rounds of suppresive PCR using primers that selectively amplify DNA hybrids representing differenitally expressed mRNAs. End product PCRs were cloned into the pGEM-T T/A cloning vector and transformed into TOP10 chemically competent cells. 958 and 171 sequences were obtained from the forward and reverse subtracted libraries, respectively.

text: Third stage larvae (cDNAs enriched from serum stimulated L3)

text: Ancylostoma caninum

text: Multiple isolates from Brisbane, Australia

text: Forward Subtracted Ancylostoma caninum L3 library

text: Plasmid

text: pGEMT-T

characteristic: vector


characteristic: INSDC last update

text: live

text: Generic

characteristic: NCBI submission model

text: Third stage larvae (cDNAs enriched from un-stimulated L3)

text: Ancylostoma caninum

text: Reverse Subtracted Ancylostoma caninum L3 library

text: Plasmid

text: pGEMT-T


text: National Tsing Hua University

text: live

characteristic: INSDC status

text: Generic

text: Bupleurum kaoi

text: Bupleurum kaoi mRNA (Lin TY)


text: NORTHEAST FORESTRY UNIVERSITY

text: live

text: Generic

characteristic: NCBI submission package

text: flower fruit stage

text: Panax ginseng

text: Leaves

characteristic: tissue-type

text: cDNA library of yellow fruit Panax ginseng leaves at flower fruit stage stage

text: pDNR-LIB


text: University of Texas at Austin

text: live

text: Generic

text: Variety O-4

characteristic: cultivar

text: Library prepared using mRNA from 7-day old etiolated seedlings

characteristic: description

text: seedling

text: E. coli

text: Corchorus olitorius

characteristic: organism

text: EcoRI

text: XhoI

text: 7-day old etiolated seedlings

text: Jute 7-day old etiolated seedlings cDNA library

text: Plasmid

text: pBluescript


characteristic: External Id

text: Institute of Chemistry. University of Sao Paulo

text: live

text: Generic

text: Cloned unidirectionally, 5' end of the cDNA cloned into SalI / NotI site of pSPORT 1. Primer: Oligo (dT). Average insert size: 1 kb; Superscript Plasmid system -5' adaptor sequence: 5'CCCACGCGTCCG3' -3' adaptor sequence: 5'GGCCGCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3'

text: Sporangium 30 minutes after induction of sporulation by starvation, exposed to heat shock (38oC) for 30 minutes

text: E. coli DH5a cells (ampicillin resistant)

characteristic: lab-host

text: Blastocladiella emersonii

text: Asexual

text: Plasmid

text: pSPORT 1


text: Medical School of Ribeirao Preto, University of Sao Paulo

text: live

text: Generic

text: A minilibrary was made by cloning products derived from ORESTES PCR (U.S.Letters Patent application No. 196,716 - Ludwig Institute for Cancer Research) profiles into the pUC 18 vector. Reverse transcription of whole animal mRNA and cDNA amplification were performed under low stringency conditions

text: mixed stage larvae, prepupae and pupae

text: Drosophila melanogaster

text: SmaI

text: SmaI

text: DL-ORESTES

text: pUC 18


text: live

text: Generic

text: embryo, larvae, pre-pupae, pupae and adult

text: Drosophila melanogaster

text: SmaI

text: SmaI

text: DP-ORESTES

text: pUC 18


text: Institute of Science and Technology

text: live

text: Generic

text: nymph

text: Gryllus bimaculatus

text: regenerating legs

text: Gryllus bimaculatus regenerating legs nymph


text: University of Parma

text: live

text: Generic

text: Isolated from the S2T strain of Scenedesmus acutus tolerant to Cr VI. Isolated after four days on Cr-free standard medium. cDNA obtained from a reverse trancription with anchored primer T12MA and isolated by Differential Display

text: Scenedesmus acutus

text: Scenedesmus acutus S2T


text: Faculty of Agriculture and Natural Science, University of Pretoria

text: live

text: Generic

text: Tugela

text: Transcript-derived fragments generated via cDNA-AFLP of wheat leaf mRNA after induction of resistance response to the Russian wheat aphid, Diuraphis noxia.

text: 2-3 leaf stage

text: Escherichia coli (JM 109)

text: leaf

characteristic: organ

text: Triticum aestivum

text: EcoRI

text: HindIII

text: cDNA-AFLP of wheat response to Diuraphis noxia in pTZ57R/T

text: plasmid


text: Institute of Hydrobiology, Chinese Academy of Sciences

text: live

text: Generic

text: Acipenser sinensis

text: female

characteristic: sex

text: pituitary

text: 24 years old female Chinese sturgeon pituitary cDNA library


text: Aarhus University

text: live

text: Generic

text: Genotype NV#20F1-39

text: Lolium perenne

text: SfiIA

text: SfiIB

text: Leaves from nitrogen depleted plant

text: Lolium perenne rg2

text: Plasmid

text: pDNR-LIB


text: Aarhus University

text: live

text: Generic

text: Genotype NV#20F1-30

text: Lolium perenne

text: SfiIA

text: SfiIB

text: Ethiolated leaf

text: Lolium perenne rg1

text: Plasmid

text: pDNR-LIB


text: Aarhus University

text: live

text: Generic

text: Genotype NV#20F1-39

text: Lolium perenne

text: SfiIA

text: SfiIB

text: Leaves from cold treated plant

text: Lolium perenne rg3

text: Plasmid

text: pDNR-LIB


text: Aarhus University

text: live

text: Generic

text: Genotype NV#20F1-39

text: Lolium perenne

text: SfiIA

text: SfiIB

text: Meristem

text: Lolium perenne rg4

text: Plasmid

text: pDNR-LIB


text: Aarhus University

text: live

text: Generic

text: Genotype NV#20F1-30

text: Lolium perenne

text: SfiIA

text: SfiIB

text: Stem

text: Lolium perenne rg5

text: Plasmid

text: pDNR-LIB


text: Aarhus University

text: live

text: Generic

text: Genotype NV#20F1-30

text: Lolium perenne

text: SfiIA

text: SfiIB

text: Leaves from drought stressed plant

text: Lolium perenne rg6

text: Plasmid

text: pDNR-LIB


text: Aarhus University

text: live

text: Generic

text: Genotype NV#20F1-30

text: Lolium perenne

text: SfiIA

text: SfiIB

text: Senescing leaves

text: Lolium perenne rg7

text: Plasmid

text: pDNR-LIB


text: Aarhus University

text: live

text: Generic

text: Genotype F6

text: Lolium perenne

text: SfiIA

text: SfiIB

text: Seedling

text: Lolium perenne sb

text: Plasmid

text: pSport1 (modified)


text: Aarhus University

text: live

text: Generic

text: Genotype F6

text: Lolium perenne

text: SfiIA

text: SfiIB

text: Seedling

text: Lolium perenne sa

text: Plasmid

text: pSport1 (modified)


text: Aarhus University

text: live

text: Generic

text: Genotype F6

text: Lolium perenne

text: SfiIA

text: SfiIB

text: Root

text: Lolium perenne r

text: Plasmid

text: pSport1 (modified)


text: Aarhus University

text: live

text: Generic

text: Genotype F6

text: Lolium perenne

text: SfiIA

text: SfiIB

text: Pollen

text: Lolium perenne p

text: Plasmid

text: pSport1 (modified)


text: Aarhus University

text: live

text: Generic

text: Genotype F6

text: Lolium perenne

text: SfiIA

text: SfiIB

text: Germinating seeds

text: Lolium perenne gsb

text: Plasmid

text: pSport1 (modified)


text: Aarhus University

text: live

text: Generic

text: Genotype F6

text: Lolium perenne

text: SfiIA

text: SfiIB

text: Germinating seeds

text: Lolium perenne gsa

text: Plasmid

text: pSport1 (modified)


text: Aarhus University

text: live

text: Generic

text: Genotype F6

text: Lolium perenne

text: SfiIA

text: SfiIB

text: Vernalized shoot

text: Lolium perenne vr

text: Plasmid

text: pSport1 (modified)


text: Aarhus University

text: live

text: Generic

text: Genotype F6

text: Lolium perenne

text: SfiIA

text: SfiIB

text: Lolium perenne ve

text: Plasmid

text: pSport1 (modified)


text: University of Puerto Rico, Rio Piedras campus

text: live

text: Generic

text: Forward unidirectional library of cDNA from regenerating intestine after 3 days post-evisceration of the Sea cucumber Holothuria glaberrima.

text: adult

characteristic: dev-stage

text: intestine

text: Holothuria glaberrima

text: EcoRI

text: XhoI

text: cDNA 3 days regenerating Intestine H. glaberrima

text: Phagemid

text: Lambda Uni-Zap XR, Bluescript SK-


text: live

text: Generic

text: Forward unidirectional library of cDNA from regenerating intestine after 7 days post-evisceration of the Sea cucumber Holothuria glaberrima.

text: adult

text: intestine

text: Holothuria glaberrima

text: EcoRI

text: XhoI

text: old cDNA 7 days regenerating intestine H. glaberrima

characteristic: title

text: Phagemid

text: Lambda Uni-Zap XR, Bluescript SK-


characteristic: INSDC center name

text: live

text: Generic

text: adult

text: Intestine

text: Holothuria glaberrima

text: EcoRI

text: XhoI

text: cDNA 7 days of Regenerating Intestine H. glaberrima

text: Phagemid

text: Lambda Uni-Zap XR, Bluescript SK-


characteristic: INSDC first public

text: live

text: Generic

text: Forward unidirectional library of cDNA from Normal (Non-eviscerated) intestine of the sea cucumber Holothuria glaberrima.

text: adult

text: intestine

text: Holothuria glaberrima

text: EcoRI

text: XhoI

text: cDNA Normal Intestine H. glaberrima

text: Phagemid

text: Lambda Uni-Zap XR, Bluescript SK-


text: live

text: Generic

text: Normalized cDNA library prepared from field-collected animals

text: Adult

text: E.coli Electromax DH10B

text: Mytilus californianus

text: Sfi1 GGCCAAATCGGCC

text: Sfi1 GGCCGCCTCGGCC

text: Male & female

text: Gill

text: Mytilus californianus library 0

text: Plasmid

text: pTrueBlue

characteristic: vector


characteristic: INSDC last update

text: live

text: Generic

text: Normalized and serially subtracted cDNA library prepared from environmentally challenged animals

text: Adult

text: E.coli Electromax DH10B

text: Mytilus californianus

text: Sfi1 GGCCAAATCGGCC

text: Sfi1 GGCCATAAGGGCC

text: Male & female

text: Gill

text: Mytilus californianus library 1

text: Plasmid

text: pFLC-I


text: University of Southern California

text: live

text: Generic

characteristic: NCBI submission model

text: Normalized and serially subtracted cDNA library prepared from muscle of environmentally challenged animals

text: Adult

text: E.coli Electromax DH10B

text: Mytilus californianus

text: Sfi1 GGCCAAATCGGCC

text: Sfi1 GGCCATAAGGGCC

text: Male & female

text: Muscle

text: Mytilus californianus library 2

text: Plasmid

text: pFLC-I


text: University of Southern California

text: live

characteristic: INSDC status

text: Generic

text: Normalized cDNA library prepared from mixed tissues of field-collected animals

text: Adult

text: E.coli Electromax DH10B

text: Mytilus californianus

text: Sfi1 GGCCATTACGGCC

text: Sfi1 GGCCGCCTCGGCC

text: Male & female

text: Gill, muscle, mantle, hepatopancreas

text: Mytilus californianus library 3

text: Plasmid

text: pTrueBlue


text: University of Southern California

text: live

text: Generic

characteristic: NCBI submission package

text: Normalized and serially subtracted cDNA library prepared from gill and muscle of environmentally challenged animals

text: Adult

text: E.coli Electromax DH10B

text: Mytilus californianus

text: Sfi1 GGCCAAATCGGCC

text: Sfi1 GGCCATAAGGGCC

text: Male & female

text: Gill and muscle

text: Mytilus californianus library 4

text: Plasmid

text: pFLC-I


text: University of Southern California

text: live

text: Generic

text: cDNA library prepared from cytoplasmic RNA from field-collected animals

text: Adult

text: E.coli Electromax DH10B

text: Mytilus californianus

text: Sfi1 GGCCATTACGGCC

text: Sfi1 GGCCGCCTCGGCC

text: Male & female

text: Gill

characteristic: tissue-type

text: Mytilus californianus library 5

text: Plasmid

text: pTrueBlue


text: University of Southern California

text: live

text: Generic

text: Normalized and serially subtracted cDNA library prepared from gill and muscle tissue of environmentally challenged animals

text: Adult

text: E.coli Electromax DH10B

text: Mytilus californianus

text: Sfi1 GGCCAAATCGGCC

text: Sfi1 GGCCATAAGGGCC

text: Male & female

text: Gill & muscle

text: Mytilus californianus library 6

text: Plasmid

text: pFLC-I


text: USDA/ARS, Crop Protection and Management Research Unit

text: live

text: Generic

characteristic: cultivar

text: cDNA library was constructed from peanut cultivar A13. A13 has resistance to Aspergillus infection and drought tolerance. Total RNA from leaves (challenged by drought stress and Aspergillus flavus inoculation) was isolated with RNeasy Kit (QIAGEN). mRNA was extracted from total RNA using Oligotex (QIAGEN). cDNA synthesis and library construction followed the protocol of ZAP Express cDNA Synthesis Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit (Stratagene). The cDNA above 300 bp was collected after size-fraction. The inserts were directionally cloned into ZAP Express vector using XhoI and EcoRI adapters. The lambda library was packed into phage using Gigapack III Gold (Stratagene). The unamplified library was used to excise pBluescript phagemids from the ZAP Express vector, and the phagemids were used to transform the host bacteria XL1-Blue MRF cells.

text: XL1-Blue MRF

text: Leaves

text: Arachis hypogaea

text: EcoRI

text: XhoI

text: Leaf

text: USDA-Tifton Peanut Library AH

text: Plasmid

text: pBlueScriptIISK+


text: live

text: Generic

text: cDNA library was constructed from peanut cultivar GT-C20. GT-C20 is susceptible to TSMV, and has reduced aflatoxin contamination. Newly expanded leaves were bulked from over thirty plants at 100 days after planting (DAP) for total RNA extraction. Total RNA was isolated with RNeasy Kit (QIAGEN). mRNA was extracted from total RNA using Oligotex (QIAGEN). cDNA synthesis and library construction followed the protocol of ZAP Express cDNA Synthesis Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit (Stratagene). The cDNA above 300 bp was collected after size-fraction. The inserts were directionally cloned into ZAP Express vector using XhoI and EcoRI adapters. The lambda library was packed into phage using Gigapack III Gold (Stratagene). The unamplified library was used to excise pBluescript phagemids from the ZAP Express vector, and the phagemids were used to transform the host bacteria XL1-Blue MRF cells.

characteristic: description

text: XL1-Blue MRF

text: Leaves

text: Arachis hypogaea

text: EcoRI

text: XhoI

text: Leaf

text: USDA-Tifton Peanut Library C20L

text: Plasmid

text: pBlueScriptIISK+


text: live

text: Generic

text: XL1-Blue MRF

text: Leaves

text: Arachis hypogaea

characteristic: organism

text: EcoRI

text: XhoI

text: Leaf

text: USDA-Tifton Peanut Library CVPL

text: Plasmid

text: pBlueScriptIISK+


characteristic: External Id

text: live

text: Generic

text: XL1-Blue MRF

text: Leaves

text: Arachis hypogaea

text: EcoRI

text: XhoI

text: Leaf

text: USDA-Tifton Peanut Library ISBL

text: Plasmid

text: pBlueScriptIISK+


text: live

text: Generic

text: XL1-Blue MRF

characteristic: lab-host

text: Leaves

text: Arachis hypogaea

text: EcoRI

text: XhoI

text: Leaf

text: USDA-Tifton Peanut Library NMPL

text: Plasmid

text: pBlueScriptIISK+


text: live

text: Generic

text: Tifrunner

text: cDNA library was constructed from peanut cultivar Tifrunner. Tifrunner is resistant to TSMV and susceptible to Aspergillus parasiticus. Total RNA from mature leaves was isolated with RNeasy Kit (QIAGEN). mRNA was extracted from total RNA using Oligotex (QIAGEN). cDNA synthesis and library construction followed the protocol of ZAP Express cDNA Synthesis Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit (Stratagene). The cDNA above 300 bp was collected after size-fraction. The inserts were directionally cloned into ZAP Express vector using XhoI and EcoRI adapters. The lambda library was packed into phage using Gigapack III Gold (Stratagene). The unamplified library was used to excise pBluescript phagemids from the ZAP Express vector, and the phagemids were used to transform the host bacteria XL1-Blue MRF cells.

text: XL1-Blue MRF

text: Leaves

characteristic: organ

text: Arachis hypogaea

text: EcoRI

text: XhoI

text: Leaf

text: USDA-Tifton Peanut Library TFL

text: Plasmid

text: pBlueScriptIISK+


text: University of Technology of Vienna, Institute of Chemical Engineering

text: live

text: Generic

text: Trichoderma lixii

characteristic: strain

text: Trichoderma harzianum K3 mycelium Lambda Zap Library L14


text: live

text: Generic

text: Trichoderma aggressivum f. europaeum

text: CBS 453.93

text: Trichoderma aggressivum f. europaeum CBS 453.93 mycelium Lambda Zap Library L14


text: live

text: Generic

text: Trichoderma asperellum

text: Trichoderma asperellum 11 mycelium Lambda Zap Library L14


text: live

text: Generic

text: Trichoderma stromaticum

text: CBS 101875

text: Trichoderma stromaticum CBS 101875 mycelium Lambda Zap Library L14


text: NRC Institute for Marine Biosciences Industrial Partnership Facility

text: live

text: Generic

text: Gadus morhua

text: head kidney

text: Gadus morhua head kidney library (injected with heat-killed Aeromonas salmonicida)


text: live

text: Generic

text: Gadus morhua

text: head kidney

text: Gadus morhua head kidney library (injected with polyriboinosinic polyribocytidylic acid)


text: live

text: Generic

text: Gadus morhua

text: head kidney

text: Gadus morhua head kidney SSH library enriched for genes up-regulated by acute thermal stress


text: live

text: Generic

text: Gadus morhua

text: head kidney

text: Gadus morhua head kidney SSH library enriched for genes down-regulated by polyriboinosinic polyribocytidylic acid (polyI:C)


text: live

text: Generic

text: Gadus morhua

text: head kidney

text: Gadus morhua head kidney SSH library enriched for genes down-regulated by acute thermal stress


text: live

text: Generic

text: Gadus morhua

text: liver

text: Gadus morhua liver SSH library enriched for genes up-regulated by acute thermal stress


text: live

text: Generic

text: Gadus morhua

text: liver

text: Gadus morhua liver SSH library enriched for genes down-regulated by acute thermal stress


text: live

text: Generic

text: Gadus morhua

text: skeletal muscle

text: Gadus morhua skeletal muscle SSH library enriched for genes up-regulated by acute thermal stress


text: live

text: Generic

text: Gadus morhua

text: spleen

text: Gadus morhua spleen SSH library enriched for genes up-regulated by polyriboinosinic polyribocytidylic acid (polyI:C)


text: live

text: Generic

text: Gadus morhua

text: spleen

text: Gadus morhua spleen SSH library enriched for genes down-regulated by polyriboinosinic polyribocytidylic acid (polyI:C)


text: live

text: Generic

text: Bos taurus

text: avascular region of epiphyseal cartilage

text: avascular region of cow epiphyseal cartilage cDNA library


text: Leibniz Institut fur Neue Materialien

text: live

text: Generic

text: Blossoms

text: Matricaria chamomilla var. recutita

text: Blossoms

text: Camomile blossoms

text: Plasmid

text: Lambda TriplEX2 (Clontech)


text: Institute for Chemical Research

text: live

text: Generic

text: synonym: Camellia sinensis var. sinensis

text: Camellia sinensis

text: leaves

text: up-regulated cDNA during the manufacturing of oolong tea


text: Peking University

text: live

text: Generic

text: Lambda Zap Express Library

text: 0-10 day post anthesis

characteristic: dev-stage

text: Gossypium hirsutum

text: EcoRI

text: XhoI

text: fiber

text: Cotton fiber 0-10 day post anthesis

text: plasmid

text: pBK-CMV


text: Pusan National University, School of Medicine

text: live

text: Generic

text: adult

text: Toxascaris leonina

text: Toxascaris leonina adult worm cDNA library

characteristic: title


text: Norwegian University of Sience and Technology

characteristic: INSDC center name

text: live

text: Generic

text: Clones obtained by subtractive hybridizaion of ovary samples of previtellogenic Atlantic cod exposed to a complex mixture of androgens

text: Previtellogenic

text: Ovary

text: Gadus morhua

text: Female

characteristic: sex

text: CodArray SSH cDNA library

text: pGEM T-easy cloning vector


text: University of British Columbia

characteristic: INSDC first public

text: live

text: Generic

text: RACE-PCR cDNA fragments obtained from Antonospora locustae

text: Dormant spores

text: Antonospora locustae

text: Spores

text: RACE-PCR products from Antonospora locustae

text: Plasmid

text: Topo TA vector

characteristic: vector


text: University of British Columbia

characteristic: INSDC last update

text: live

text: Generic

characteristic: NCBI submission model

text: RACE-PCR cDNA fragments obtained from Encephalitozoon cuniculi

text: Dormant spores

text: Encephalitozoon cuniculi

text: Spores

text: RACE-PCR products from Encephalitozoon cuniculi

text: Plasmid

text: Topo TA vector


text: Hygiene Institution

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission package

text: fibroblast

characteristic: cell type

characteristic: cell-line

text: cDNA sequence from Homo sapiens WI38 cells treated with PM2.5

text: lung

text: Homo sapiens

text: female

text: bronchus

text: Human PM2.5 treated WI-38 cDNA

text: pDrive Cloning Vector


text: Institute of Applied Science and Technology (ICAT)

text: live

text: Generic

text: The cDNA library was constructed from mRNA purified from roots inoculated with fungal mycelium (6h and 12 h post inoculation) using the SMART cDNA Library Construction Kit (BD Clontech). cDNAs were cloned into pTriplEx2 (BD Clontech).

characteristic: description

text: symbiotic, 6-12 h after contact with fungal mycelium

text: E.coli

text: Castanea sativa

characteristic: organism

text: Castanea sativa X Pisolithus tinctorius early interaction (6h and 12h) - Castanea sativa

text: plasmid


characteristic: External Id

text: live

text: Generic

text: symbiotic, 6-12 h after contact with roots

text: E.coli

characteristic: lab-host

text: Pisolithus tinctorius

text: Castanea sativa X Pisolithus tinctorius early interaction (6h and 12h) - Pisolithus tinctorius

text: plasmid


text: National Cancer Institute / NIH

text: live

text: Generic

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer 5'-GACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3', double-stranded cDNA was cloned into the NotI and EcoRV sites of pExpress-1 (EcoRV site destroyed upon ligation). Library was size-selected for an average insert size of 1.92 kb. Primary library, non-amplified. Use M13(-21) to generate 3' end reads and M13R to generate 5' end reads. Library was constructed by Open Biosystems (Huntsville, AL). Note: this is a Zebrafish Gene Collection (http://zgc.nci.nih.gov/) library.

text: DH10B TonA

text: fin

characteristic: organ

text: Danio rerio

text: EcoRV

text: NotI

text: fin blastema

characteristic: tissue-type

text: plasmid


text: National Cancer Institute / NIH

text: live

text: Generic

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer 5'-GACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3', double-stranded cDNA was cloned into the NotI and EcoRV sites of pExpress-1 (EcoRV site destroyed upon ligation). Library was size-selected for an average insert size of 1.85 kb. Primary library, non-amplified. Use M13(-21) to generate 3' end reads and M13R to generate 5' end reads. Library was constructed by Open Biosystems (Huntsville, AL). Note: this is a Zebrafish Gene Collection (http://zgc.nci.nih.gov/) library.

text: DH10B TonA

text: fin

text: Danio rerio

text: EcoRV

text: NotI

text: early outgrowth fin

characteristic: title

text: plasmid


text: Institut de Recherche pour le Developpement (IRD)

characteristic: INSDC center name

text: live

text: Generic

text: Gills

text: Subtracted libraries were constructed with the PCR-select cDNA subtraction kit (Clontech). The subtraction was realised between mRNAs of Gills from fish exposed to hypersaline water and freshwater osmotic shock. The cDNA fragments obtained were cloned into the pCR2.1 vector (Invitrogen) and electroporated into DH10B cells.

text: Juvenile

characteristic: dev-stage

text: Sarotherodon melanotheron

text: subtracted libraries from gill of black-chinned tilapia exposed to hypersaline water and freshwater osmotic shock

text: pCR(r)2.1-TOPO cloning vector from TOPO TA cloning kit (Invitrogen)


text: University of California

characteristic: INSDC first public

text: live

text: Generic

characteristic: cultivar

text: Seeds of IT84S-2049 were from a single plant with a long history of single seed descent (Ehlers). The types of tissue were: 1) germinated seed hypocotyl and primary root, 2) germinated seed epicotyl and foliage leaves, 3 & 4) young trifoliate leaves and apical meristem (one well watered, one drought stressed). For germinated seed samples (1 & 2), seeds were surface sterilized 5 minutes in 1% sodium hypochlorite with 0.01% Tween 20, rinsed with water, germinated on sterile filter paper with water containing 100 units/ml nystatin and 50 microgram/ml cefotaxime, incubated 4-5 days in the dark at room temperature (Fenton). For samples 3 & 4, seeds were planted in 3 gallon pots and kept watered in a glasshouse until plants were ~25 cm in height (~3 weeks)(Fenton). For drought stress (sample 4), water was withheld until visible signs of drought stress occurred (reduced growth relative to watered plants, wilted leaves, less green color), generally ~3 weeks. Total RNA was purified using a TRIzol method (Fenton). RNA was pooled to establish one highly complex RNA sample with a total of 1000 microgram, as follows: 333 microgram of hypocotyl & primary root (sample 1), 333 microgram of epicotyl and foliage leaves (sample 2) and 167 microgram each of stressed and unstressed young trifoliate leaves and apical meristem (samples 3 & 4)(Fenton). PolyA RNA was purified using Promega PolyATtrack mRNA Isolation System IV (Fenton). Five microgram of poly(A) mRNA was used for cDNA synthesis. A primary cDNA library was produced using a Stratagene lambda ZAP XR cDNA Synthesis Kit (Fenton). 250 thousand pfu from the primary library were mass excised to produce a phagemid population (Fenton). Phagemids were plated (Fenton, Resnik), plasmid DNA purified, cDNA clones archived, and DNA sequences determined bi-directionally using an ABI3730 at the Genomics Core Instrumentation facility, University of California, Riverside (Choi). Trace files were downloaded (Close) and then processed (Wanamaker) using the HarvEST pipeline (http://harvest.ucr.edu) to remove vector and cloning oligo sequences and various contaminants, and to trim to a high quality region. Sequences that retained a phred 17 region of at least 100 bases were deposited to GenBank with annotations (Close).

text: Seedling

text: E. coli TJC121

text: Vigna unguiculata

text: EcoRI

text: XhoI

text: mixed

text: Cowpea IT84S-2049 cDNA Library UCRVU03-1

text: Phagemid pBluescript SK(-)

text: Lambda Uni-ZAP XR, excised phagemid


text: The Samuel Roberts Noble Foundation

characteristic: INSDC last update

text: live

text: Generic

text: Nicotiana benthamiana plants were grown on compost in the greenhouse with supplemental lighting (4 h in winter) providing a minimum illumination period of 14 h. Trichomes were harvested from stems of 5 week old N. benthamiana plants taken from the top half of the shoot. The stems were placed in 50 ml disposable falcon tubes and trichomes were abraded by vortexing for 20 s after freezing in liquid nitrogen. RNA was isolated using the Qiagen RNeasyMini kit. A cDNA EST library was generated following manufacturers instructions using the Creator SMART cDNA library construction kit (BD Biosciences) starting from total RNA and using the pDNR-LIB vector.

text: Apical stem internodes of 5-week old plants.

text: Nicotiana benthamiana

text: Sfi I A (GGCCATTACGGCC)

text: Sfi I B (GGCCGCCTCGGCC)

text: Isolated stem trichomes

text: Nicotiana benthamiana trichomes

text: pDNR_lib (Clontech)

characteristic: vector


text: The Samuel Roberts Noble Foundation

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Solanum lycopersicum plants were grown on compost in the greenhouse with supplemental lighting (4 h in winter) providing a minimum illumination period of 14 h. Trichomes were harvested from the most apical leaves of 5 week old Solanum lycopersicum plants. Trichomes were harvested by brushing leaves frozen using liquid nitrogen and separated from contaminating leaf fragments by passing the frozen material through a sieve. RNA was isolated from the trichomes using the Qiagen RNeasyMini kit. A cDNA EST library was generated following manufacturers instructions using the Creator SMART cDNA library construction kit (BD Biosciences) starting from total RNA and using the pDNR-LIB vector.

text: Apical internodes of 5-week old plants.

text: Solanum lycopersicum

text: Sfi I A (GGCCATTACGGCC)

text: Sfi I B (GGCCGCCTCGGCC)

text: Isolated leaf trichomes

text: Solanum lycopersicum trichomes

text: pDNR_lib (Clontech)


text: UMR CNRS-UPS 5546

text: live

text: Generic

characteristic: NCBI submission package

text: Eucalyptus globulus EST from juvenile versus mature wood and mature versus juvenile wood SSH libraries

text: Eucalyptus globulus subsp. bicostata

text: RAIZ clone VC9

characteristic: strain

text: differentiating xylem

text: Eucalyptus globulus juvenile and mature wood SSH library


text: live

text: Generic

text: Drimiopsis kirkii

text: Drimiopsis kirkii SSH


text: Volcani Center, Agriculture Research Organization (ARO)

text: live

text: Generic

text: perlette

text: Mature

text: Bud

text: Vitis vinifera

text: Vitis vinifera cv. perlette LibA


text: live

text: Generic

text: perlette

text: Mature

text: Bud

text: Vitis vinifera

text: Vitis vinifera cv. perlette LibB


text: live

text: Generic

text: perlette

text: Mature

text: Bud

text: Vitis vinifera

text: Vitis vinifera cv. perlette LibC

text: pBluescript SK(+/-)


text: live

text: Generic

text: perlette

text: Young

text: Bud

text: Vitis vinifera

text: Vitis vinifera cv. perlette LibD

text: pBluescript SK(+/-)


text: Plant Biotechnology Institute, National Research Council Canada

text: live

text: Generic

text: Sequences have been trimmed to remove vector and low quality regions.

characteristic: description

text: Brassica napus

text: Brassica napus 10FGYSUA


text: live

text: Generic

text: Brassica napus

characteristic: organism

text: Brassica napus 11FGYSDB


characteristic: External Id

text: live

text: Generic

text: Brassica napus

text: Brassica napus 13JKBNM2


text: live

text: Generic

text: Brassica napus

text: Brassica napus 40JKME7D


text: The Plant Biotechnology Institute, National Research Council Canada

text: live

text: Generic

text: Brassica napus

text: Brassica napus 44JK5DM


text: National Research Council of Canada

text: live

text: Generic

text: Brassica oleracea

text: Brassica oleracea 46RDOAG


text: National Research Council of Canada

text: live

text: Generic

text: Brassica oleracea

text: Brassica oleracea 47RDOAH


text: National Research Council of Canada

text: live

text: Generic

text: Brassica oleracea

text: Brassica oleracea 48RDOAM


text: National Research Council of Canada

text: live

text: Generic

text: Brassica oleracea

text: Brassica oleracea 49RDOAT


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Brassica napus 4ETGS72H


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Brassica napus 50ETGS2H


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Brassica napus 51ETGS4H


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Brassica napus 53COT2


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Brassica napus 53COT5


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Brassica napus 54ACDHPE


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Brassica napus 55ACACPE


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Brassica napus 56ACAC48


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Brassica napus 57ACPE6


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Brassica napus 58ACPE48


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Brassica napus 5ETGS9H


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Brassica napus 60ACAB48


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Brassica napus 62ETGS52


text: live

text: Generic

text: Brassica napus

text: Brassica napus 63JKCOT5


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Brassica napus 64ETCOT5


text: live

text: Generic

text: Brassica napus

text: Brassica napus 65JKBNM0


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Brassica napus 66ETFLGS


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Brassica napus 67ETGS


text: live

text: Generic

text: Brassica napus

text: Brassica napus 69JKME5D


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Brassica napus 6ETGS6H


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Brassica napus 70ETGS6H


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Brassica napus 71ETGS12


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Brassica napus 72ETGS24


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Brassica napus 73ETGS36


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Brassica napus 74ETGS48


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Brassica napus 75ETGS60


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Brassica napus 76ETGS72


text: National Research Council of Canada

text: live

text: Generic

text: Brassica carinata

text: Brassica carinata 7RDBCH


text: National Research Council of Canada

text: live

text: Generic

text: Brassica rapa

text: Brassica rapa 8RDBRH


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Brassica napus 9RDBNGA


text: National Research Council of Canada

text: live

text: Generic

text: Brassica carinata

text: Brassica carinata BCXiang2


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Brassica napus BNAEN1GH


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Brassica napus BNAEN2GH


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Brassica napus BNAEN3GH


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Brassica napus BNARO1GH


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Brassica napus BNARO5GH


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Brassica napus BNARO6GH


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Brassica napus BNARO2GH


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Brassica napus BNARO4GH


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Brassica napus BNBS2DCT


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Brassica napus BNBS4DCT


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Brassica napus BNBS6DCT


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Brassica napus BNBS8DCT


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Brassica napus BNDH2DCT


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Brassica napus BNDH4DCT


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Brassica napus BNHA


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Brassica napus BNSCS1CT


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Brassica napus BNSCS2CT


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Brassica napus BNSCS3CT


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Brassica napus BNSCS4CT


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Brassica napus BNSCS5CT


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Brassica napus BNYS2DCT


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Brassica napus BNYS4DCT


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Brassica napus BNYS6DCT


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Brassica napus BNYS8DCT


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Brassica napus BNZDA


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Brassica napus DHBN6DCT


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Brassica napus DHBN8DCT


text: live

text: Generic

text: Brassica napus

text: Brassica napus EX20LIB10


text: live

text: Generic

text: Brassica napus

text: Brassica napus EX20LIB7


text: live

text: Generic

text: Brassica napus

text: Brassica napus EX20LIB8


text: live

text: Generic

text: Brassica napus

text: Brassica napus EX20LIB9


text: live

text: Generic

text: Brassica napus

text: Brassica napus EX24ATES


text: live

text: Generic

text: Brassica napus

text: Brassica napus EX24L2


text: live

text: Generic

text: Brassica napus

text: Brassica napus EX24L3


text: live

text: Generic

text: Brassica napus

text: Brassica napus EX30LIB31


text: live

text: Generic

text: Brassica napus

text: Brassica napus EX30LIB33


text: live

text: Generic

text: Brassica napus

text: Brassica napus EX30LIB34


text: live

text: Generic

text: Brassica napus

text: Brassica napus EX50LIB5


text: live

text: Generic

text: Brassica napus

text: Brassica napus EX50LIB7


text: live

text: Generic

text: Brassica napus

text: Brassica napus EX50LIB9


text: live

text: Generic

text: Brassica napus

text: Brassica napus FNYFDA


text: live

text: Generic

text: Brassica napus

text: Brassica napus FNYFDB


text: live

text: Generic

text: Brassica napus

text: Brassica napus FNYFDC


text: live

text: Generic

text: Brassica napus

text: Brassica napus FNYFDD


text: live

text: Generic

text: Brassica napus

text: Brassica napus FNYSD


text: live

text: Generic

text: Brassica napus

text: Brassica napus FNYSU


text: live

text: Generic

text: Brassica napus

text: Brassica napus FTYFDA


text: live

text: Generic

text: Brassica napus

text: Brassica napus FTYFDB


text: live

text: Generic

text: Brassica napus

text: Brassica napus FTYFDC


text: live

text: Generic

text: Brassica napus

text: Brassica napus JKBNHS2


text: live

text: Generic

text: Brassica napus

text: Brassica napus JKBNM3


text: Agriculture and Agri-food Canada

text: live

text: Generic

text: DH12075 (double haploid line from Cresor x Westar cross)

text: The library was made from apical meristem (young plants). poly-A primed library using SuperScript Plasmid System with Gateway Technology for cDNA Synhesis and Cloning kit (Invitrogen). The sequence has been processed using Lucy.

text: Brassica napus

text: Not I

text: Sal I

text: Apical meristem

text: Brassica napus Apical meristem

text: pSPORT1 (modified: GCGGCCGCCc*GACTAGTGAGCTC*cggacgcgtggGTCGAC)


text: Agriculture and Agri-food Canada

text: live

text: Generic

text: The library was made from small to medium sized healthy leaves. poly-A primed library using SuperScript Plasmid System with Gateway Technology for cDNA Synhesis and Cloning kit (Invitrogen). The sequence has been processed using Lucy.

text: Brassica napus

text: Not I

text: Sal I

text: Leaf blade

text: Brassica napus Leaf library


text: Agriculture and Agri-food Canada

text: live

text: Generic

text: The library was made from the first true leaves that were dead or dying. poly-A primed library using SuperScript Plasmid System with Gateway Technology for cDNA Synhesis and Cloning kit (Invitrogen). The sequence has been processed using Lucy.

text: Brassica napus

text: Not I

text: Sal I

text: Leaf blade

text: Brassica napus Senescent leaves


text: Agriculture and Agri-food Canada

text: live

text: Generic

text: The library was made from hydroponically grown root tissue. RNA was provided by Dr. Good, University of Alberta. poly-A primed library using SuperScript Plasmid System with Gateway Technology for cDNA Synhesis and Cloning kit (Invitrogen). The sequence has been processed using Lucy.

text: Brassica napus

text: Not I

text: Sal I

text: Root

text: Brassica napus Root library


text: Agriculture and Agri-food Canada

text: live

text: Generic

text: The library was made from plant stem tissue cut between growth nodes (auxillary buds were not included). poly-A primed library using SuperScript Plasmid System with Gateway Technology for cDNA Synhesis and Cloning kit (Invitrogen). The sequence has been processed using Lucy.

text: Brassica napus

text: Not I

text: Sal I

text: Stem

text: Brassica napus Stem library


text: Agriculture and Agri-food Canada

text: live

text: Generic

text: The library was made from fresh flowers in early stage of development. Most of the flower receptacle tissue had been removed. poly-A primed library using SuperScript Plasmid System with Gateway Technology for cDNA Synhesis and Cloning kit (Invitrogen). The sequence has been processed using Lucy.

text: Brassica napus

text: Not I

text: Sal I

text: Developed flowers

text: Brassica napus Flower


text: Agriculture and Agri-food Canada

text: live

text: Generic

text: Anthers were extracted from developing buds when they were 0.5 to 1 mm in size. Tissue was collected by Dr. Kevin Rozwadowski and Tricia Kreiser. poly-A primed library using SuperScript Plasmid System with Gateway Technology for cDNA Synhesis and Cloning kit (Invitrogen). The sequence has been processed using Lucy.

text: Brassica napus

text: Not I

text: Sal I

text: Anther

text: Brassica napus Very early anther


text: Agriculture and Agri-food Canada

text: live

text: Generic

text: Anthers were extracted from developing buds when they were 2 to 3 mm in size. Tissue was collected by Dr. Kevin Rozwadowski and Tricia Kreiser. poly-A primed library using SuperScript Plasmid System with Gateway Technology for cDNA Synhesis and Cloning kit (Invitrogen). The sequence has been processed using Lucy.

text: Brassica napus

text: Not I

text: Sal I

text: Anther

text: Brassica napus Early anther


text: Agriculture and Agri-food Canada

text: live

text: Generic

text: The library was made from apical and lateral buds of a range of sizes and ages. poly-A primed library using SuperScript Plasmid System with Gateway Technology for cDNA Synhesis and Cloning kit (Invitrogen). The sequence has been processed using Lucy.

text: Brassica napus

text: Not I

text: Sal I

text: Floral Buds

text: Brassica napus Bud library