characteristic: INSDC last update

text: live

text: Generic

text: JURKAT CELL LINE

text: Homo sapiens

text: T CELLS (JURKAT CELL LINE)

text: Homo sapiens T CELLS (JURKAT CELL LINE)


status: PUBLIC

characteristic: External Id

text: Genoscope - Centre National de Sequencage

characteristic: INSDC center name

characteristic: INSDC first public

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: 1st strand cDNA was primed with a NotI-oligo(dT) primer. Five prime end enriched, double-strand cDNA was digested with Not I and cloned into the Not I and EcoRV sites of the pCMVSPORT 6 vector. Library was not normalized.

characteristic: description

text: fetal

tag: attribute

characteristic: dev-stage

text: liver

characteristic: organ

text: Homo sapiens

characteristic: organism

text: FETAL LIVER

characteristic: tissue-type

text: Homo sapiens FETAL LIVER

characteristic: title

characteristic: vector


text: live

text: Generic

text: adult

text: brain

text: Homo sapiens

text: ADULT BRAIN

text: Homo sapiens ADULT BRAIN


text: Harbin Institute of Technology; Harbin Gene-tech Biochip Development Inc., Ltd.

text: live

text: Generic

text: Myofiber

characteristic: cell type

text: This library was constructed from 7 million independent clones. cDNA synthesis was initiated using an oligo(dT) primer, using methylated C in the first strand synthesis reaction. Following this first strand reaction, double-stranded cDNA was blunted, ligated to EcoRI adapters, digested with XhoI, size-selected, and cloned into the EcoRI and XhoI compatible sites of a custom modified lambda ZAP-CMV vector. Then the recombinant vector was packaged into Gigapack III Gold packaging extract. The primary library was amplified to 70 million with the XL1-Blue MRF' cells as host strain and excised into the recombinant pCMV-Script EX phagemid vector with the help of ExAssist helper phage and XLOLR strain. And the excised library contains 24 million independent clones.

text: 6 week

text: XL-blue MRF'

characteristic: lab-host

text: breast

text: Gallus gallus

text: EcoRI

text: XhoI

text: male

characteristic: sex

text: Layer

characteristic: strain

text: skeletal muscle

text: chicken Lambda ZAP-CMV XR Library

text: Phagemid

text: pCMV-Script EX


characteristic: External Id

characteristic: INSDC last update

text: live

text: Generic

text: Hanxuan 10

characteristic: cultivar

text: Hanxuan 10 wheat (Triticum aestivum L.) seedlings were cultivated in water at 20oC with 12h light every day until one and a half leaves at Key Laboratory of Crop Germplasm & Biotechnology, Ministry of Agriculture, Institute of Crop Germplasm Resources, Chinese Academy of Agricultural Sciences, Beijing 100081,China. Then a part of seedlings were continuously cultivated in water for 48h as control (the driver), another part of seedlings were stressed in PEG-6000-0.5MPa solution for 48h as treatment (the tester). The tissue, total RNA, and poly(A) RNA were prepared, A subtracted cDNA library was constructed by suppression subtractive hybridization (SSH).

text: one and a half leaves

text: E. coli

text: Triticum aestivum

text: Leaf

text: Wheat water-stressed seedling SSH library

text: pGEM-Teasy


text: Instituto de Quimica - Universidade de Sao Paulo

characteristic: INSDC center name

characteristic: INSDC first public

text: live

characteristic: INSDC status

text: Generic

text: adult

text: Mesocricetus auratus

text: Schistosoma mansoni

text: mixed pool

text: Plasmid


text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: adult

text: Mesocricetus auratus

text: Schistosoma mansoni

text: mixed pool

text: Plasmid

text: SureClone


text: live

text: Generic

text: adult

characteristic: dev-stage

text: Mesocricetus auratus

text: Schistosoma mansoni

text: mixed pool

text: Plasmid

text: TopoBlunt


text: live

text: Generic

text: adult

text: Mesocricetus auratus

text: Schistosoma mansoni

characteristic: organism

text: mixed pool

text: Plasmid

text: TopoTA


text: live

text: Generic

text: adult

text: Mus musculus

text: Schistosoma mansoni

text: mixed pool

text: Plasmid


text: live

text: Generic

text: adult

text: Mus musculus

text: Schistosoma mansoni

text: mixed pool

text: Plasmid

text: SureClone


text: live

text: Generic

text: adult

text: Mus musculus

text: Schistosoma mansoni

text: mixed pool

text: Plasmid

text: TopoBlunt


text: live

text: Generic

text: adult

text: Mus musculus

text: Schistosoma mansoni

text: mixed pool

text: Plasmid

text: TopoTA


text: live

text: Generic

text: adult

text: Mus musculus

text: Schistosoma mansoni

text: mixed pool

text: Plasmid

text: pGEM T-easy


text: live

text: Generic

text: cercaria

text: Biomphalaria glabrata

text: Schistosoma mansoni

text: mixed pool

text: Plasmid


text: live

text: Generic

text: cercaria

text: Biomphalaria glabrata

text: Schistosoma mansoni

text: mixed pool

text: Plasmid

text: TopoBlunt


text: live

text: Generic

text: cercaria

text: Biomphalaria glabrata

text: Schistosoma mansoni

text: mixed pool

text: Plasmid

text: pGEM T-easy


text: live

text: Generic

text: cercaria

text: Biomphalaria glabrata

text: Schistosoma mansoni

text: mixed pool

text: Plasmid

text: TopoTA


text: live

text: Generic

text: egg

text: Mus musculus

text: Schistosoma mansoni

text: mixed pool

text: Plasmid

text: pGEM T-easy


text: live

text: Generic

text: egg

text: Mus musculus

text: Schistosoma mansoni

text: mixed pool

text: Plasmid

text: TopoTA


text: live

text: Generic

text: egg

text: Mus musculus

text: Schistosoma mansoni

text: mixed pool

text: Plasmid

text: TopoBlunt


text: live

text: Generic

text: egg

text: Mus musculus

text: Schistosoma mansoni

text: mixed pool

text: Plasmid


text: live

text: Generic

text: adult

text: Mesocricetus auratus

text: Schistosoma mansoni

text: female

text: Plasmid

text: SureClone


text: live

text: Generic

text: adult

text: Mesocricetus auratus

text: Schistosoma mansoni

text: female

text: Plasmid

text: TopoTA


text: live

text: Generic

text: adult

text: Mesocricetus auratus

text: Schistosoma mansoni

text: female

text: Plasmid


text: live

text: Generic

text: germball

text: Biomphalaria glabrata

text: Schistosoma mansoni

text: mixed pool

text: Plasmid

text: pGEM T-easy


text: live

text: Generic

text: miracidium

text: Schistosoma mansoni

text: mixed pool

text: Plasmid

text: pGEM T-easy


text: live

text: Generic

text: miracidium

text: Schistosoma mansoni

text: mixed pool

text: Plasmid


text: live

text: Generic

text: miracidium

text: Schistosoma mansoni

text: mixed pool

text: Plasmid

text: TopoBlunt


text: live

text: Generic

text: adult

text: Mesocricetus auratus

text: Schistosoma mansoni

text: male

text: Plasmid

text: SureClone


text: live

text: Generic

text: adult

text: Mesocricetus auratus

text: Schistosoma mansoni

text: male

text: Plasmid


text: live

text: Generic

text: adult

text: Mesocricetus auratus

text: Schistosoma mansoni

text: male

text: Plasmid

text: TopoBlunt


text: live

text: Generic

text: adult

text: Mesocricetus auratus

text: Schistosoma mansoni

text: male

text: Plasmid

text: TopoTA


text: live

text: Generic

text: adult

text: Mesocricetus auratus

text: Schistosoma mansoni

text: male

text: Plasmid

text: pGEM T-easy


text: live

text: Generic

text: schistosomulum

text: in vitro culture

text: Schistosoma mansoni

text: mixed pool

text: Plasmid

text: pGEM T-easy


text: live

text: Generic

text: schistosomulum

text: in vitro culture

text: Schistosoma mansoni

text: mixed pool

text: Plasmid


text: National Cancer Institute / NIH

text: live

text: Generic

text: Library is oligo-dT primed and directionally cloned (EcoRV site is destroyed upon cloning). Average insert size 1.68 kb. Library was constructed by (Invitrogen). Note: this is a NIH_MGC Library.

characteristic: description

text: DH10B-Ton A ( T1 and T5 phage resistances)

text: Testis

characteristic: organ

text: Homo sapiens

text: NotI

text: EcoRV (destroyed)

text: plasmid (ampicillin resistant)


text: National Cancer Institute / NIH

text: live

text: Generic

text: Library is oligo-dT primed and directionally cloned (EcoRV site is destroyed upon cloning). Average insert size 1.42 kb. Library was constructed by (Invitrogen). Note: this is a NIH_MGC Library.

text: Homo sapiens

text: NotI

text: EcoRV (destroyed)

text: White Matter

characteristic: tissue-type

characteristic: title

text: plasmid (ampicillin resistant)

characteristic: vector


text: University of Edinburgh

text: live

text: Generic

text: The library was constructed by Laetitia LeGeoff in Judith Allen's laboratory at ICAPB, University of Edinburgh. The library is directional, with the 5' end of inserts adjacent to the Sal I site; sequencing with the T7 primer thus gives 5' ESTs.

text: adult

text: Litomosoides sigmodontis

text: Sal I

text: Not I

text: male

characteristic: sex

text: Litomosoides sigmodontis adult male 1

text: plasmid


text: The University of Georgia, Department of Plant Biology

text: live

text: Generic

text: The library was prepared from polyA+ RNA from 6-day-old seedlings grown in hydroponic culture and heat-shocked at 40-42 C for 4 or 24 hr. After heat shock, roots and leaves were harvested and tissues combined for RNA isolation. Double-stranded cDNA was cloned unidirectionally into different DraIII sites of the pME18S-FL3 vector (5-prime DraIII site is CACTGTGTG, 3-prime DraIII site is CACCATGTG).

text: DH10B-T1 phage-resistant E. coli

characteristic: lab-host

text: Sorghum bicolor

text: XhoI

text: XhoI

text: Heat-shocked seedlings

text: Plasmid


characteristic: External Id

text: University of Delaware

text: live

text: Generic

text: E.coli EMDH10B

text: Gallus gallus

text: Male and Female

text: fat

text: Chicken Fat cDNA library (pft1c)


text: University of Delaware

characteristic: INSDC last update

text: live

text: Generic

text: E.coli EMDH10B

text: Gallus gallus

text: Male and Female

text: liver

text: Normalized chicken liver cDNA library (pgl1n)

text: pCMVSPORT 6


text: University of Delaware

text: live

text: Generic

text: Library made from equivalent pools of total RNA isolated from each developmental age (across strains) Single pass sequencing from 5'-end

text: Embryonic (d18,d19); post-hatch (d1,w3,w7,w9,w16,1yr)

text: E. Coli EMDH10B

text: Gallus gallus

text: Male and Female

text: Commercial broiler, Ottawa Research Centre strains, leghorn

characteristic: strain

text: Abdominal Fat

text: Primary chicken abdominal fat cDNA library (pgf2c)


text: University of Delaware

characteristic: INSDC center name

text: live

text: Generic

text: E. Coli EMDH10B

text: Gallus gallus

text: Male and Female

text: Abdominal Fat

text: Normalized chicken abdominal fat cDNA library (pgf2n)


text: University of Delaware

characteristic: INSDC first public

text: live

text: Generic

text: Library made from equivalent pools of total RNA isolated from each tissue (embryonic muscle 33.3%; juvenile muscle 33.3%; and epiphyseal growth plate 33.3% of the final RNA pool). Single pass sequencing from 5'-end

text: Breast,leg:Embryo(d19);post-hatch(1d,1,3,5,7,9,11 weeks);growth plate(1d,7d,14d post-hatch)

text: E. Coli EMDH10B

text: Gallus gallus

text: Male and Female

text: Commercial broiler chickens, Ottawa Research Ctr Strains 90 & 21

text: Breast muscle, leg muscle and epiphyseal growth plate

text: Primary chicken muscle cDNA library (pgm1c)


text: University of Delaware

text: live

characteristic: INSDC status

text: Generic

text: E. Coli EMDH10B

text: Gallus gallus

text: Male and Female

text: Normalized chicken muscle cDNA library (pgm1n)


text: University of Delaware

text: live

text: Generic

characteristic: NCBI submission model

text: E. Coli EMDH10B

text: Gallus gallus

text: Male and Female

text: Normalized chicken muscle cDNA library (pgm2n)


text: University of Delaware

text: live

text: Generic

characteristic: NCBI submission package

text: Library made from equivalent pools of total RNA isolated from each tissue at different ages Single pass sequencing from 5'-end

text: Embryonic (d12,d14,d19); post-hatch (w1,w3,w5,w7,w9)

text: E. Coli EMDH10B

text: Gallus gallus

text: Male and Female

text: Commercial broiler chicken

text: Pituitary Gland/Hypothalamus/Pineal Gland

text: Primary chicken pituitary/hypothalamus/pineal cDNA library (pgp1c)


text: University of Delaware

text: live

text: Generic

characteristic: dev-stage

text: E. Coli EMDH10B

text: Gallus gallus

text: Male and Female

text: Commercial broiler chicken

text: Normalized chicken pituitary/hypothalamus/pineal cDNA library (pgp1n)


text: University of Delaware

text: live

text: Generic

text: Library made from equivalent pools of total RNA isolated from each tissue at different ages. Single pass sequencing from 5'-end

text: Embryonic (d12,d14,d19); post-hatch (1,3,5,7,9 weeks)

text: E. Coli EMDH10B

text: Gallus gallus

characteristic: organism

text: Male and Female

text: Commercial broiler chickens

text: Normalized chicken pituitary/hypothalamus/pineal cDNA library (pgp2n)


text: University of Delaware

text: live

text: Generic

text: E.coli EMDH10B

text: Gallus gallus

text: Male and Female

text: fat

text: Normalized chicken fat cDNA library (pgf1n)


text: Peking University Third Hospital

text: live

text: Generic

text: expressed higher in keloid than in hypertrophic scar

characteristic: description

text: keloid for 10 years

text: skin

text: Homo sapiens

text: female

text: Homo sapiens keloid cDNA

text: Plasmid

text: PGEM-T Easy


text: Peking University, Health Science Center

text: live

text: Generic

text: fibroblast

characteristic: cell type

characteristic: cell-line

text: embryo

text: lung

characteristic: organ

text: Homo sapiens

text: female

text: Human fibroblasts senescence upregulated subtracted library


text: live

text: Generic

text: fibroblast

text: embryo

text: lung

text: Homo sapiens

text: female

text: Human fibroblasts senescence downregulated subtracted library

characteristic: title


text: Washington University School of Medicine

text: live

text: Generic

text: The cDNA library was constructed by Keliang Tang, and Robert Cole at Washington University. cDNA was synthesized from Poly(A)+ mRNA using an oligo-d(T) primer containing a XhoI site. Following second strand synthesis, EcoRI adapters were ligated to the cDNA, and products were size-selected on sephacryl S500. The cDNA were directionally cloned into the EcoRI/XhoI prepared pBluescript II SK+ vector, and electroporated into ElectroTen Blue cells (Stratagene). The library may contain a small percentage of host or bacterial contaminants.

text: Tachyzoite

text: ElectroTen Blue cells (Stratagene)

text: Toxoplasma gondii

text: EcoRI

text: XhoI

text: Tachyzoite

text: TgMAS Tachyzoite cDNA Library

text: pBluescript II SK+

characteristic: vector


text: Oklahoma State University

text: live

text: Generic

text: Petit havana

characteristic: cultivar

text: PCR amplified partial cDNAs generated using suppressive subtractive hybridization enriching for mRNAs present in the light but not when in the presence of photosynthetic electron transport inhibitor DCMU, cloned into pGEM by TA cloning

text: 3 week old

text: Nicotiana tabacum

text: leaf

characteristic: tissue-type

text: Tobacco light-enhanced cDNA library

text: plasmid

text: PGEM


characteristic: External Id

text: University of Delaware

characteristic: INSDC last update

text: live

text: Generic

text: Library made from three total RNA pools from each tissue (testis 25%, ovary 25%, and oviduct 50% of final RNA pool) Single pass sequencing from 5'-end

text: Various stages;embryonic, post-hatch, immature and sexually-mature

text: E. Coli EMDH10B

characteristic: lab-host

text: Gallus gallus

text: Male and Female

characteristic: sex

text: Commercial broiler and layer

text: Testis, ovary and oviduct

text: Normalized chicken reproductive tract cDNA library (pgr1n)


text: USDA/ARS

characteristic: INSDC center name

characteristic: INSDC first public

text: live

characteristic: INSDC status

text: Generic

text: embryo sac

text: Embryo sacs were isolated with enzymatic maceration and manual microdissection. RNA was extracted from the embryo sacs. A cDNA library was constructed by oligo dT priming using the Clontech SMART cDNA synthesis kit. The cDNAs were directionally cloned.

text: Zea mays

text: Zea mays embryo sac cDNA library

text: Clontech Triplex2


text: US Department of Agriculture, Agriculture Research Service, Pacific West Area, Western Regional Research Center

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Cheyenne

text: Seeds collected, Cheyenne endosperm isolated, and RNA prepared by Susan Altenbach. Library constructed by Stratagene, Inc. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab.

text: 5 -30 days post anthesis seed

characteristic: dev-stage

text: E. coli SOLR

text: Triticum aestivum

text: EcoRI

text: Cheyenne endosperm

text: Cheyenne wheat endosperm cDNA library

text: Phagemid

text: Lambda ZAP II, excised phagemid


text: live

text: Generic

text: Cheyenne

text: Seeds collected, endosperm isolated, and RNA prepared by Susan Altenbach. Library constructed by Stratagene, Inc. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab. The library was enriched for low-copy cDNA sequences by removing highly abundant cDNAs using colony hybridization.

characteristic: description

text: 5 -30 days post anthesis seed

text: E. coli SOLR

text: Triticum aestivum

characteristic: organism

text: EcoRI

text: Endosperm

text: Wheat subtracted endosperm cDNA library

text: Phagemid

text: Lambda ZAP II, excised phagemid


text: National Cancer Institute / NIH

text: live

text: Generic

text: ORFs were PCR-amplified and cloned into pcDNA3.1 by the GPCR Consortium. Cloning sites vary by clone and include the following: 5'-EcoRV-XmnI/XhoI-3', 5'-EcoRV-XmnI/NotI-3', EcoRV (TA cloned, non-directional). For information about which gene each clones represents, please visit our anonymous ftp site at ftp://image.llnl.gov/image/rearrayed_plates/IRBI.preSV.data Note: this is a NIH_MGC Library.

text: Homo sapiens

text: varies by clone

text: varies by clone

text: mixed

text: plasmid (ampicillin-resistant)


text: The University of Georgia, Department of Plant Biology

text: live

text: Generic

text: The library was prepared from a mixture of polyA+ RNA from callus culture tissue and cells in suspension culture. Double-stranded cDNA was cloned unidirectionally into different DraIII sites of the pME18S-FL3 vector (5-prime DraIII site is CACTGTGTG, 3-prime DraIII site is CACCATGTG). XhoI excises the cDNA insert.

text: DH10B-T1 phage-resistant E. coli

text: Sorghum bicolor

text: XhoI

text: XhoI

text: Callus culture/cell suspension

text: Plasmid


text: live

text: Generic

text: The library was prepared from polyA+ RNA from 9-day-old seedlings grown in hydroponic culture. Seedlings were transferred to a 150 mM NaCl solution and harvested at 3, 6, 12 and 24 hr following transfer. Roots and leaves were pooled from all time points and RNA isolated. Double-stranded cDNA was cloned unidirectionally into different DraIII sites of the pME18S-FL3 vector (5-prime DraIII site is CACTGTGTG, 3-prime DraIII site is CACCATGTG).

text: DH10B-T1 phage-resistant E. coli

text: Sorghum bicolor

text: XhoI

text: XhoI

text: Salt-stressed seedlings

text: Plasmid


text: Chinese National Human Genome Center at Shanghai

text: live

text: Generic

text: fetal

text: Homo sapiens

text: sfiIA

text: sfiIBl

text: pituitary

text: FNP


text: King Faisal Specialist Hospital and Research Centre

text: live

text: Generic

text: Monocytes

characteristic: cell type

characteristic: cell-line

text: The library was prepared from LPS-stimulated cell line pre-treated with cycloheximide.

text: Homo sapiens

text: THP-1 OligodT Library

text: Plasmid

text: PT7BLUE2 blunt cloning vector (Novagen)


text: live

text: Generic

text: Monocytes

text: The library was enriched for AU-rich transcripts from LPS-stimulated THP-1 monocytes

text: Homo sapiens

text: THP-1 TAP Library

characteristic: title

text: PT7BLUE2 blunt cloning vector (Novagen)

characteristic: vector


text: Wageningen University

text: live

text: Generic

text: protoplasts in the stage of cell wall biosynthesis

text: leaf

characteristic: organ

text: Solanum americanum

text: leaf protoplast cDNA fragments isolated by cDNA-AFLP


characteristic: External Id

text: Virginia Bioinformatics Institute

text: live

text: Generic

text: mycelium

text: Phytophthora infestans

text: SalI

text: NotI

text: mycelium

characteristic: tissue-type

text: Wageningen/U.Maryland Phytophthora infestans EST Project

text: Plasmid


text: Virginia Bioinformatics Institute

characteristic: INSDC last update

text: live

text: Generic

text: mycelium

text: Phytophthora sojae

text: EcoRI

text: XhoI

text: mycelium

text: sMA: Phytophtora sojae grown in synthetic medium

text: Phagemid

text: pBK-CMV


text: Virginia Bioinformatics Institute

text: live

text: Generic

text: mycelium

text: Phytophthora sojae

text: SalI

text: NotI

text: sMC: Phytophtora sojae grown in synthetic medium

text: Plasmid


text: Microbial Ecology, Institution of Ecology

text: live

text: Generic

text: This EST clone is originating from one of three cDNA libraries, constructed for transcript profiling of the mycorrhizal interaction between the basidiomycete Paxillus involutus and Betula pendula (birch). One library represents the developed and functional mycorrhizal root tissue ('(FHIG:A) Ectomycorrhiza plate culture'), a second library represents axenically grown fungus ('(FHIG:B) Axenic plate culture') and a third library represents axenically grown plants ('(FHIG:C) Axenic plate culture'). Libraries were analyzed in parallel and 3555 (FHIG:A), 3964 (FHIG:B), and 2532 (FHIG:C) high-quality (PHRED 20) ESTs of >99bp have been deposited. The cDNA libraries were constructed from total RNA using the SMART cDNA library Construction kit (#K1051-1, Clontech, Palo Alto, CA, USA) according to the manufacturer's instructions. Full-length cDNAs were trimmed by SfiI, fractionated and directionally ligated into (lambda)TriplEx2 arms. The lambda library was converted to a plasmid library via site-specific recombination at loxP sites in a Cre+ strain (E. coli BM25.8). Plasmid clones were randomly collected and analysed by DNA sequencing using a plasmid-specific forward primer (P104).

text: 25 days of growth after transfer/synthesis

text: Escherichia coli BM25.8

characteristic: lab-host

text: Betula pendula/Paxillus involutus mixed EST library

text: SfiI

text: SfiI

text: ATCC200175(P.involutus);Skuleskogen,Skogsforsk,Sweden(B.pendula)

characteristic: strain

text: Ectomycorrhizal root tissue

text: (FHIG:A) Ectomycorrhiza plate culture

text: Plasmid


characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: 25 days of growth after transfer

text: Escherichia coli BM25.8

text: Paxillus involutus

text: SfiI

text: SfiI

text: ATCC 200175

text: Mycelium

text: (FHIG:B) Axenic plate culture

text: Plasmid


text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: 25 days of growth after transfer

text: Escherichia coli BM25.8

text: Betula pendula

text: SfiI

text: SfiI

text: Skuleskogen, Skogsforsk, Sweden

text: Root tissue

text: (FHIG:C) Axenic plate culture

text: Plasmid


text: Texas A&M University

text: live

text: Generic

characteristic: NCBI submission package

text: Non-normalized library, sequenced 5' with Triplex2 primer (CTCCGAGATCTGGACGAGC). Library constructed by Clontech with total RNA extracted using the Trizol method and pooled from 5 females.

text: Day 14 pregnant

characteristic: dev-stage

text: uterus

text: Ovis aries

text: EcoRI

text: XhoI

text: Female

characteristic: sex

text: endometrium

text: POE14_(Day_14_pregnant_ovine_endometrium)

text: Plasmid: pTriplex2


text: Max-Planck-Institut fuer Molekulare Genetik

text: live

text: Generic

text: Random primed and directionally cloned in pSport1 vector using a NotI (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC (T)15-3' and a SalI 5'- TCGACCCACGCGTCCG-3'adapters (Gibco BRL)

characteristic: description

text: larva 2-3 weeks

text: E.coli, XL1 blue

text: Strongylocentrotus purpuratus

characteristic: organism

text: NotI

text: SalI

text: whole larva

text: Sea urchin larva cDNA library MPMGp691

text: Plasmid


text: live

text: Generic

text: embryonic 0hr

text: E.coli, XL1 blue

text: Strongylocentrotus purpuratus

text: NotI

text: SalI

text: whole unfertilised eggs

text: Sea urchin unfertilised egg cDNA library MPMGp621

characteristic: title

text: Plasmid


text: live

text: Generic

text: embryonic 7hr

text: E.coli, XL1 blue

text: Strongylocentrotus purpuratus

text: NotI

text: SalI

text: whole embryo

text: Sea urchin embryo 7hr cleavage stage cDNA library MPMGp538

text: Plasmid

characteristic: vector


text: live

text: Generic

text: embryonic 40hr

text: E.coli, XL1 blue

text: Strongylocentrotus purpuratus

text: NotI

text: SalI

text: whole embryo

text: Sea urchin embryo 40hr gastrula stage cDNA library MPMGp536

text: Plasmid


text: live

text: Generic

text: embryonic 20hr

text: E.coli, XL1 blue

text: Strongylocentrotus purpuratus

text: NotI

text: SalI

text: whole embryo

text: Sea urchin embryo 20hr blastula stage cDNA library MPMGp537

text: Plasmid


text: Washington University School of Medicine

text: live

text: Generic

text: Sporozoites were obtained from in vitro sporulated and excysted oocysts of E.tenella grown in chickens. cDNA was synthesized from poly mRNA using an oligo-dT primer containing a XhoI site. Following second strand synthesis, EcoRI adapters were ligated to the cDNA and products were size-selected on sephacryl S500. The cDNA were ligated to EcoRI/XhoI prepared lambda ZapII (Stratagene). The primary library was mass excised using ExAssist helper phage (Stratagene). The phagemids were preciptated with PEG 8000, extracted with phenol/chloroform and electroporated into DH10B cells. The library may contain a small percentage of host or bacterial contaminants. Library materials provided by: Paul Liberator, Merck Research Labs Library constructed by: Paul Liberator, Merck Research Labs.

text: E.coli DH10B (GeneHog, Invitrogen,Inc)

text: Eimeria tenella

text: EcoRI

text: XhoI

text: Eimeria tenella M5-6 Excised cDNA

text: plasmid

text: pBluescript SK-


text: Rajiv Gandhi Center for Biotechnology

text: live

text: Generic

text: The cDNA library was constructed from young leaves of Andrographis paniculata Nees in the flowering stage. Total RNA was isolated from young leaves of flowering plant. mRNA was isolated from the total RNA after DNase I treatment. cDNA synthesis carried out using Time saver cDNA synthesis kit and Directional Cloning Toolbox from Amersham Pharmacia Biotech with 3_g mRNA as starting material and Not I/oligo-dT18 primer ( 5'd [AAC TGG AAG AAT TCG CGG CCG CAG GAAT18] p 3') according to the instruction of the manufacturer. After the second strand synthesis, cDNA was purified by phenol: chloroform extraction and size fractionated using SepharoseTM CL-4B spun columns. Eco RI adaptor was ligated to the size fractionated cDNA followed by Not I digestion. The cDNA was treated with T4 Polynucleotide Kinase after Not I digestion and Phenol: chloroform extracted followed by spun column purification. The eluted cDNA was ligated to the Lambda ExCell Not I/Eco RI/ CIP vector (Directionl Cloning Toolbox, Amersham Pharmacia Biotech) which facilitate directional cloning. Lambda DNA along with the ligated cDNA was packaged using Ready-To-Go Lambda Packaging Kit (Amersham Pharmacia Biotech). pExCell was excised from a packaged lambda ExCell library using NP66 host cells. The released plasmids were retransformed to JM109 cells by transformation and plasmids from each clones were isolated separately for size selection of inserts and sequencing. Size selection was carried out by PCR using vector specific primers T7 and SP6. Sequencing was done with SP6 primer using Big Dye Terminator Sequencing Kit from Perkin Elmer. Sequencing was done in ABI Prism 310.This library was constructed in Plant Molecular Biology Laboratory of Rajiv Gandhi Centre for Biotechnology under the guidance of Dr. Nilam Sanjib Banerjee.

text: flowering stage

text: E.coli NM522

text: Andrographis paniculata

text: Eco RI

text: Not I

text: young leaf

text: Andrographis paniculata Nees cDNA library from young leaves of the flowering plant

text: Phage

text: Lambda ExCell


text: Shanghai Institute of Planned Parenthood Research

text: live

text: Generic

text: The Clontech PCR-Select cDNA subtraction kit was used to construct the ESTs library according to its user manual. Tester ds cDNA were prepared from the mRNA sample of toad oocytes derived at low temperature. Driver ds cDNA were prepared from the mRNA samples of toad oocytes derived at high temperature.

text: Stage VI

text: TOP10F' E. coli

text: Bufo gargarizans

text: female

text: oocyte

text: Subtractive library of toad oocyte at high-low temperature

text: pT-Adv


text: Horticulture College of Northwest Sci-Tech University of Agriculture and Forestry

text: live

text: Generic

characteristic: cultivar

text: These sequences are differential display cDNAs of Baihe-35-1 leaves which were infected with the fungus Uncinula necator in August,2002, in Yangling Shannxi, China. These ESTs was screening by DDRT-PCR. The expression of them was high in the infected leaves while not found in the healthy leaves. Plants were symptomatic at the time of collection.

text: Vitis pseudoreticulata

text: female

text: leaves

text: Baihe-35-1 leaves DDRT-PCR


characteristic: External Id

text: live

text: Generic

text: The library was constructed according Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. Denatured RNA was size fractionated on a 1% agarose gel.First strand cDNA synthesis was primed with oligo-dT primer containing a Not I site.Double strand cDNA was size selected according to mRNA size fraction ,ligated with EcoR I adaptor , digested with NotI and then cloned directionally into pYX-Asc vector. The library tag sequence located between the Not I site and the polyA tail is CGAACTGAAT. This library was created for the University Iowa Brain Anatomy Project (BMAP): 'Gene Discovery in the Developing Mouse Nervous System', supported by National Institute of Mental Health (NIMH), Hemin Chin , Ph.D. , program coordinator.

text: 1, 5 and 15 days newborn

text: DH10B (T1 phage resistant)

text: Brain

characteristic: organ

text: Mus musculus

text: EcoR I

text: Not I

text: Whole brain

text: Plasmid

text: pYX- Asc


characteristic: INSDC last update

text: live

text: Generic

text: The library was constructed according Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. Denatured RNA was size fractionated on a 1% agarose gel.First strand cDNA synthesis was primed with oligo-dT primer containing a Not I site.Double strand cDNA was size selected according to mRNA size fraction,ligated with EcoR I adaptor, digested with NotI and then cloned directionally into pYX-Asc vector. The library tag sequence located between the Not I site and the polyA tail is CGAACTGAAT. This library was created for the University Iowa Brain Anatomy Project (BMAP): 'Gene Discovery in the Developing Mouse Nervous System', supported by National Institute of Mental Health (NIMH), Hemin Chin, Ph.D., program coordinator.

text: 1, 5 and 15 days newborn

text: DH10B (T1 phage resistant)

text: Brain

text: Mus musculus

text: EcoR I

text: Not I

text: whole brain

characteristic: tissue-type

text: Plasmid

text: pYX- Asc


text: Children's Memorial Research Center

text: live

text: Generic

text: UI-R-GO0 is a cDNA library containing the following tissue(s): rat whole embryo 13dpc. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. Denatured RNA was size fractionated on a 1% agarose gel. First strand cDNA synthesis was primed with oligo-dT primer containing a Not I site. Double strand cDNA was size selected according to mRNA size fraction, ligated with EcoR I adaptor, digested with NotI and then cloned directionally into pYX-Asc vector. The library tag sequence located between the Not I site and the polyA tail is CATCTCTACT. This library was created for the University of Iowa Program for Rat Gene Discovery and Mapping (Val Sheffield, Bento Soares and Tom Casavant).

text: embryo 13dpc

text: DH10B (Life Technologies) (T1 phage resistant)

characteristic: lab-host

text: Rattus norvegicus

text: EcoR I

text: Not I

text: Sprague-Dawley

characteristic: strain

text: Whole embryo

text: Plasmid

text: pYX-Asc


text: Children's Memorial Research Center

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: UI-R-GR0 is a cDNA library containing the following tissue(s): rat whole embryo 13dpc. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. Denatured RNA was size fractionated on a 1% agarose gel. First strand cDNA synthesis was primed with oligo-dT primer containing a Not I site. Double strand cDNA was size selected according to mRNA size fraction, ligated with EcoR I adaptor, digested with NotI and then cloned directionally into pYX-Asc vector. The library tag sequence located between the Not I site and the polyA tail is CATCTCTACT. This library was created for the University of Iowa Program for Rat Gene Discovery and Mapping (Val Sheffield, Bento Soares and Tom Casavant).

text: embryo 13dpc

text: Rattus norvegicus

text: EcoR I

text: Not I

text: Sprague-Dawley

text: Whole embryo

text: Plasmid

text: pYX-Asc


text: US Department of Agriculture, Agriculture Research Service, Pacific West Area, Western Regional Research Center

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Chinese Spring

text: Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Shoots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

text: Five day old seedling

text: E. coli SOLR

text: Triticum aestivum

text: EcoRI

text: XhoI

text: Shoot; etiolated

text: CS wheat unstressed etiolated seedling shoot cDNA library

text: Phagemid

text: Lambda Uni-ZAP XR, excised phagemid


text: live

text: Generic

characteristic: NCBI submission package

text: Chinese Spring

text: Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Five-day old seedlings were incubated for one day at 90% RH. After removing endosperm, seedlings were transferred to desiccator jar containing saturated MgSO4 at room temperature for 24 hr. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

text: Five day old seedling

characteristic: dev-stage

text: E. coli SOLR

text: Triticum aestivum

text: EcoRI

text: XhoI

text: Seedling without endosperm

text: CS wheat drought-stressed seedling cDNA library

text: Phagemid

text: Lambda Uni-ZAP XR, excised phagemid


text: live

text: Generic

text: Chinese Spring

text: Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Five-day old seedlings were transferred to 5 C cold room and kept for 48 hr. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

characteristic: description

text: Five-day old seedling

text: E. coli SOLR

text: Triticum aestivum

text: EcoRI

text: XhoI

text: Seedling

text: CS wheat cold-stressed seedling cDNA library

text: Phagemid

text: Lambda Uni-ZAP XR, excised phagemid


text: live

text: Generic

text: Chinese Spring

text: Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Roots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

text: Five day old etiolated seedling

text: E. coli SOLR

text: Triticum aestivum

characteristic: organism

text: EcoRI

text: XhoI

text: Root

text: CS wheat etiolated seedling root cDNA library

text: Phagemid

text: Lambda Uni-ZAP XR, excised phagemid


text: live

text: Generic

text: Plants were grown in the greenhouse. Spikes were sprayed at anthesis with Fusarium graminearum. Total RNA, and poly(A) RNA were prepared and pooled from infected spike at 0, 6, 12, 24, 36 and 48 hours after inoculation, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in G. Muehlbauer lab at the University of Minnesota (Kruger, W.M., Muehlbauer, G.J., Pritsch, C., Vance, C.). The cDNA library should contain genes of both wheat and fungal pathogen origin. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

text: Adult plant

text: E. coli SOLR

text: Triticum aestivum

text: EcoRI

text: XhoI

text: Spike

text: Sumai3 Wheat Fusarium graminearum infected spike cDNA library

characteristic: title

text: Phagemid

text: Lambda Uni-ZAP XR, excised phagemid pBluescript SK


text: Institute of Genetics and Developmental Biology, CAS, China

text: live

text: Generic

text: Kefeng 1

text: The cDNA library was constructed by He, C-Y from mRNA isolated from two-week seedlings (cultivar Kefeng 1) treated by spraying 2.0mM salicylic acid for 24, 36, 48 and 72 h. Complementary DNA was synthesized from mRNA using a primer consisting of a poly(dT) sequence with a XhoI striction site. EcoRI adapters were ligated to the blunt-ended cDNA fragments followed by XhoI digestion. The cDNA fragments were directionally cloned into the EcoRI-XhoI restriction site of the pBluescript vector. The ligated cDNA fragments were transformed into XL1-Blue MRF' host cells (Stratagene).

text: two-week seedlings

text: XL1-Blue MRF' strain

text: Glycine max

text: EcoR I

text: Xho I

text: Seedlings

text: Soybean induced by Salicylic Acid

text: Phage

text: pBluescript SK+

characteristic: vector


text: The University of Georgia, Department of Plant Biology

text: live

text: Generic

characteristic: cultivar

text: The library was prepared from polyA+ RNA from seedlings grown in hydroponic culture. At 8 days of age, medium was supplemented with 1 mM salicylic acid (SA). Roots and shoots were harvested after 27 and 72 hr and material from both time points was combined prior to RNA isolation. Double-stranded cDNA was cloned unidirectionally into different DraIII sites of the pME18S-FL3 vector (5-prime DraIII site is CACTGTGTG, 3-prime DraIII site is CACCATGTG). XhoI excises the cDNA insert.

text: DH10B-T1 phage-resistant E. coli

text: Sorghum bicolor

text: XhoI

text: XhoI

text: Salicylic acid-treated seedlings

text: Plasmid


characteristic: External Id

text: live

text: Generic

text: The library was prepared from polyA+ RNA from seedlings grown in hydroponic culture. At 8 days of age, medium was supplemented with 0.5 mM 1-aminocyclopropane-1-carboxylic acid (ACC) to induce endogenous ethylene (ETH) production. Roots and shoots were harvested after 27 and 72 hr and material from both time points was combined prior to RNA isolation. Double-stranded cDNA was cloned unidirectionally into different DraIII sites of the pME18S-FL3 vector (5-prime DraIII site is CACTGTGTG, 3-prime DraIII site is CACCATGTG). XhoI excises the cDNA insert.

text: DH10B-T1 phage-resistant E. coli

text: Sorghum bicolor

text: XhoI

text: XhoI

text: Ethylene-treated seedlings

text: Plasmid


text: Waksman Institute, Rutgers University

text: live

text: Generic

text: Zea mays

text: EcoRI

text: XhoI

text: Endosperm of 7-23DAP


text: Waksman Institute, Rutgers University

text: live

text: Generic

text: Zea mays

text: EcoRI

text: XhoI

text: Endosperm of 4-6 DAP

text: pBluscript SK-


text: Waksman Institute, Rutgers University

text: live

text: Generic

text: Zea mays

text: EcoRI

text: XhoI

text: Endosperm of 7-23 DAP

text: pBK-CMV


text: University of Edinburgh

text: live

text: Generic

text: Tardigrades (also know as water bears) are small free living animals inhabiting marine, fresh water and water film habitats. Hypsibius dujardini is a freshwater species maintained in lab culture. The library was prepared from adults and juveniles and was constructed by Jennifer Daub, University of Edinburgh.

text: mixed (adult and juvenile)

text: Hypsibius dujardini

text: Sal I (5'end)

text: Not I (3'end)

text: parthenogenic

characteristic: sex

text: whole body

text: Hypsibius dujardini mixed stage (fraction 7)

text: plasmid


text: University of Edinburgh

characteristic: INSDC last update

text: live

text: Generic

text: mixed (adult and juvenile)

text: Hypsibius dujardini

text: Sal I (5'end)

text: Not I (3'end)

text: parthenogenic

text: whole body

text: Hypsibius dujardini mixed stage (fraction 8)

text: plasmid


text: US Department of Agriculture, Agriculture Research Service, Pacific West Area, Western Regional Research Center

text: live

text: Generic

text: Chinese Spring

text: Plants were grown in the greenhouse. Spikes at 5, 10 and 15 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

text: Adult plant

text: E. coli SOLR

text: Triticum aestivum

text: EcoRI

text: XhoI

text: Spike

characteristic: tissue-type

text: CS wheat 5-15 DAP spike cDNA library

text: Phagemid

text: Lambda Uni-ZAP XR, excised phagemid


text: live

text: Generic

text: Chinese Spring

text: Plants were grown in the greenhouse. Whole spike with awns trimmed, white, green and yellow anther were collected and total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

text: Adult plant

text: E. coli SOLR

characteristic: lab-host

text: Triticum aestivum

text: EcoRI

text: XhoI

text: Spike before anthesis

text: CS wheat pre-anthesis spike cDNA library

text: Phagemid

text: Lambda Uni-ZAP XR, excised phagemid


text: live

text: Generic

text: Chinese Spring

text: Spikes at 5, 10, 15 and 20 days after anthesis were heat stressed under two conditions at Texas Tech University (D. Zhang in HT Nguyrn lab): (1) at 38 C for 4 hours and (2) 5 days of cyclic treatment of 38 C for 4 hours. Total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton, Malatrasi) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

text: E. coli SOLR

text: Triticum aestivum

text: EcoRI

text: XhoI

text: Whole spike

text: CS wheat heat stressed spike cDNA library

text: Phagemid

text: Lambda Uni-ZAP XR, excised phagemid


text: live

text: Generic

text: Chinese Spring

text: Plants were given a gradual stress down to 80%, 70% and to 60% RWC at Texas Tech University (D. Zhang in HT Nguyen lab). Total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab at the University of California, Riverside (Akhunov, Chin, Choi, Close, Fenton, Kianian, Otto, Simons, Zhang). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

text: Full tillering stage

text: E. coli SOLR

text: Triticum aestivum

text: EcoRI

text: XhoI

text: Leaf

text: CS wheat drought stressed leaf cDNA library

text: Phagemid

text: Lambda Uni-ZAP XR, excised phagemid


text: live

text: Generic

text: Chinese Spring

text: Plants were grown under hydroponic conditions at UC Davis, salt stressed for 12 hours, and for 7 days, then dissected and frozen (Akhunov in J Dvorak Lab). Total RNA was prepared from sheath tissue, equal quantities of RNA were pooled from the two samples, polyA was purified from the pooled RNA, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab at the University of California, Riverside (Akhunov, Chin, Choi, Close, Fenton, Kianian, Otto, Simons, Zhang). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

text: Adult plant

text: E. coli SOLR

text: Triticum aestivum

text: EcoRI

text: XhoI

text: Sheath

text: CS wheat salt-stressed sheath cDNA library

text: Phagemid

text: Lambda Uni-ZAP XR, excised phagemid


text: live

text: Generic

text: Chinese Spring

text: Plants were grown under hydroponic conditions at UC Davis, salt stressed for 12 hours, and for 7 days, then dissected and frozen (Akhunov in J Dvorak Lab). Total RNA and poly(A) RNA were prepared from crown tissue, equal portions of RNA were pooled from the two treatments, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab at the University of California, Riverside (Akhunov, Chin, Choi, Close, Fenton, Kianian, Otto, Simons, Zhang). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

text: Adult plant

text: E. coli SOLR

text: Triticum aestivum

text: EcoRI

text: XhoI

text: Crown

text: CS wheat salt-stressed crown cDNA library

text: Phagemid

text: Lambda Uni-ZAP XR, excised phagemid


text: live

text: Generic

text: Plants were grown in a growth chamber at North Dakota State University (Kianian, Otto, Simons). Tissues collected from seven-day etiolated seedling leaf, stem, root and seed; leaf from plant at fourth leaf stage; spike from pre-anthesis through 20 days after anthesis; flag leaf; leaf and stem tissue from tillers. and root. Total RNA and poly(A) RNA were prepared from each tissue and then pooled, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Akhunov, Chin, Choi, Close, Fenton, Kianian, Otto, Simons, Zhang) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

text: Different growth stages

text: E. coli SOLR

text: Triticum turgidum

text: EcoRI

text: XhoI

text: All tissues

text: Triticum turgidum L. var. durum (durum wheat) Langdon-16 whole plant cDNA library

text: Phagemid

text: Lambda Uni-ZAP XR, excised phagemid


text: live

text: Generic

text: Chinese Spring

text: Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Roots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. The cDNA clones were in vivo excised to give pBluescript phagemids before normalization was carried out. The mass excision of phagemid library and normalization were done in HT Nguyen lab by D. Zhang at Texas Tech Univeristy. Normalization protocol used was that of Soares'. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

text: Five day old seedling

text: E. coli DH10B

text: Triticum aestivum

text: EcoRI

text: XhoI

text: Etiolated shoot

text: CS wheat unstressed seedling shoot normalized cDNA library

text: Phagemid

text: Lambda Uni-ZAP XR, excised phagemid pBluescript SK


text: US Department of Agriculture, Agriculture Research Service, Pacific West Area, Western Regional Research Center

text: live

text: Generic

text: Chinese Spring

text: Five day old etiolated seedling

text: E. coli DH10B

text: Triticum aestivum

text: EcoRI

text: XhoI

text: Root

text: CS wheat etiolated seedling root normalized cDNA library

text: Phagemid


text: live

text: Generic

text: Blanco

text: Plants were grown in the greenhouse. Anthers were harvested and pooled from early meiosis to late meiosis. The tissue, total RNA, and poly(A) RNA were prepared (Butler, Ross and Gustafson) at University of Missouri, Columbia. A cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

text: Adult plant before anthesis

text: E. coli SOLR

text: Secale cereale

text: EcoRI

text: XhoI

text: Anther

text: Rye anther cDNA library

text: Phagemid

text: Lambda Uni-ZAP XR, excised phagemid


text: live

text: Generic

text: TAM W101

text: Plants were given a gradual stress down to 65% and 78% RWC at Texas Tech University (D. Zhang in HT Nguyen lab). Total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close), at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

text: Full tillering stage

text: E. coli SOLR

text: Triticum aestivum

text: EcoRI

text: XhoI

text: Leaf

text: TAM W101 wheat drought stressed leaf cDNA library

text: Phagemid

text: Lambda Uni-ZAP XR, excised phagemid


text: live

text: Generic

text: Chinese Spring

text: Plants at full-tillering stage were heat stressed under four conditions: (1) 38 C for 2 hours, (2) 38 C for 4 hours, (3) 2 days of cyclic treatment of 38 C for 2 hours, (4) 2 days of cyclic treatment of 38 C for 4 hours, at Texas Tech University (D Zhang in Nguyen lab). Total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton, Malatrasi) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

text: Full tillering stage

text: E. coli SOLR

text: Triticum aestivum

text: EcoRI

text: XhoI

text: Flag leaf

text: CS wheat heat stressed flag leaf cDNA library

text: Phagemid

text: Lambda Uni-ZAP XR, excised phagemid


text: live

text: Generic

text: Chinese Spring

text: Two-week old seedling were heat treated at 37 C for 2 hr and cyclic heat treated at 37 C for 2 hr for two days at Texas Tech University (D. Zhang, H. Nguyen lab). The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

text: Two-week old seedling

text: E. coli SOLR

text: Triticum aestivum

text: EcoRI

text: XhoI

text: Leaf

text: CS wheat heat-stressed seedling cDNA library

text: Phagemid

text: Lambda Uni-ZAP XR, excised phagemid


text: live

text: Generic

text: Chinese Spring

text: Seeds were germinated and grown at 4 C for 5 weeks. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

text: Five-week old seedling

text: E. coli SOLR

text: Triticum aestivum

text: EcoRI

text: XhoI

text: Crown tissue of seedling

text: CS wheat vernalized crown cDNA library

text: Phagemid

text: Lambda Uni-ZAP XR, excised phagemid


text: live

text: Generic

text: Chinese Spring

text: Plants were grown in the greenhouse. Spikes at 20 DAP and seeds at 30 to 45 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

text: Adult plant

text: E. coli SOLR

text: Triticum aestivum

text: EcoRI

text: XhoI

text: Spike and seed

text: CS wheat 20-45 DAP spike cDNA library

text: Phagemid

text: Lambda Uni-ZAP XR, excised phagemid


text: Agriculture and Agri-food Canada

text: live

text: Generic

text: Fusarium graminearum grown in liquid culture to optimize trichothecene production; final deoxynivalenol concentration in culture determined by CD-ELISA to be 165 micrograms/ml. cDNA was prepared using a modified SSH protocol to promote normalization whereby cDNA was not digested prior to hybridization and no subtraction was attempted

text: Asexual

text: E. coli SURE2

text: Fusarium graminearum

text: EcoRI

text: DAOM 180378

characteristic: strain

text: Mycelium

text: Fg03_AAFC_ECORC_Fusarium_graminearum_mycelium_trichothecene_production

text: Plasmid

text: pGEM-T easy


text: Agriculture and Agri-food Canada

characteristic: INSDC center name

text: live

text: Generic

text: Fusarium graminearum grown on cornmeal solid substrate in the dark for 14 days at 25 C. cDNA library prepared using Invitrogen GeneRacer kit.

text: Asexual

text: E. coli SURE

text: Fusarium graminearum

text: EcoRI

text: DAOM 180378

text: Mycelium

text: Fg05_AAFC_ECORC_Fusarium_graminearum_mycelium_cornmeal_solid_culture

text: Plasmid

text: pGEM-T easy


text: Agriculture and Agri-food Canada

characteristic: INSDC first public

text: live

text: Generic

text: Fusarium grown in a 0.4% dextrin/0.1% NH4Cl liquid culture, shaking at 220 rpm in the dark for 7 days.cDNA library prepared using Invitrogen GeneRacer kit.

text: Asexual

text: E. coli SURE

text: Fusarium graminearum

text: EcoRI

text: DAOM 180378

text: stressed mycelium tissue

text: Fg10_AAFC_ECORC_Fusarium_graminearum_dextrin-stressed

text: Plasmid

text: pGEM-T easy


text: Agriculture and Agri-food Canada

text: live

characteristic: INSDC status

text: Generic

text: Fusarium grown on wheat (cv. Roblin) under high humidity. cDNA made using Stratagene kit.

text: Asexual

text: E. coli

text: Fusarium graminearum

text: EcoRI

text: XhoI

text: DAOM 180378

text: Mycelium

text: Fg04_AAFC_ECORC_Fusarium_graminearum_mycelium_grown_on_wheat_heads

text: Plasmid

text: Bluescript SK+


text: Agriculture and Agri-food Canada

text: live

text: Generic

characteristic: NCBI submission model

text: Fusarium graminearum grown on a complex plant substrate-- wheat leaves treated to remove most of the low molecular weight, water-soluble components.

text: Asexual

text: E. coli DH10B

text: Fusarium graminearum

text: EcoRI

text: XhoI

text: DAOM 180378

text: Mycelium

text: Fg08_AAFC_ECORC_Fusarium_graminearum_complex_substrate

text: Plasmid

text: pBluescript II+


text: Agriculture and Agri-food Canada

text: live

text: Generic

characteristic: NCBI submission package

text: Fusarium graminearum grown on a simple substrate-- minimal media supplemented with amino acids.

text: Asexual

text: E. coli DH10B

text: Fusarium graminearum

text: EcoRI

text: XhoI

text: DAOM 180378

text: Mycelium

text: Fg09_AAFC_ECORC_Fusarium_graminearum_simple_substrate

text: Plasmid

text: pBluescript II+


text: Penn State University

text: live

text: Generic

text: Only floral buds with diameter of 2.5 mm or less were used for RNA isolation. This is a directionally cloned, non-normalized library. Avg. insert length: 800; Primers: M13F and M13R; Antibiotic: 50 ug/ml Ampicillin; Primary Titer: 1E6 pfu total; Amplified Titer: 1.4E10 pfu/ml; Mass Excised Titer: 6.4E7 total; This library has been generated by the Floral Genome Project (FGP). The Floral Genome Project is funded by NSF's Plant Genome Research Program (DBI-0115684). More information about the project can be obtained at http://fgp.bio.psu.edu

text: <= 2.5mm buds

characteristic: dev-stage

text: SOLR

text: Nuphar advena

text: EcoRI

text: flower buds

text: Phagemid

text: pBluescript SK (+/-)


text: Penn State University

text: live

text: Generic

text: Amborella trichopoda Baill; This library was made from male flowers only. Only floral buds with diameter of 2.5 mm of less were used for RNA isolation. This is a directionally cloned, non-normalized library. Avg. insert length: 1611; Primers: M13F and M13R; Antibiotic: 50 ug/ml Ampicillin; Primary Titer: 2.24E6 pfu total; Amplified Titer: 1.37E10 pfu/ml; Mass Excised Titer: 3.53E9 total; This library has been generated by the Floral Genome Project (FGP). We would like to thank David Lorence at the National Tropical Botanical Garden for providing plant material for library building. The Floral Genome Project is funded by NSF's Plant Genome Research Program (DBI-0115684). More information about the project can be obtained at http://fgp.bio.psu.edu

characteristic: description

text: <= 2.5mm buds

text: SOLR

text: Amborella trichopoda

text: EcoRI

text: flower buds

text: Phagemid

text: pBluescript SK (+/-)


text: Penn State University

text: live

text: Generic

text: Only floral buds with diameter of 2.5 mm of less were used for RNA isolation. This is a directionally cloned, non-normalized library. Avg. insert length: 1134; Primers: M13F and M13R; Antibiotic: 50 ug/ml Ampicillin; Primary Titer: 2E6 pfu total; Amplified Titer: 3.2E10 pfu/ml; Mass Excised Titer: 5E10 total; This library has been generated by the Floral Genome Project (FGP). The Floral Genome Project is funded by NSF's Plant Genome Research Program (DBI-0115684). More information about the project can be obtained at http://fgp.bio.psu.edu

text: <= 2.5mm

text: SOLR

text: Nuphar advena

characteristic: organism

text: EcoRI

text: flower buds

text: Phagemid

text: pBluescript SK (+/-)


text: Penn State University

text: live

text: Generic

text: Aurantiaca Orange

text: Plants were grown in greenhouse at Penn State from commercially available seeds. Only floral buds with diameter of 2.5 mm of less were collected. This is a directionally cloned, non-normalized library. Avg. insert length: 1702; Primers: M13F and M13R; Antibiotic: 50 ug/ml Ampicillin; Primary Titer: 7E6 pfu total; Amplified Titer: 1.68E11 pfu/ml; Mass Excised Titer: 5.6E8 total; This library has been generated by the Floral Genome Project (FGP). We would like to thank Huck Life Sciences Consortium for their assistance. The Floral Genome Project is funded by NSF's Plant Genome Research Program (DBI-0115684). More information about the project can be obtained at http://fgp.bio.psu.edu

text: millimeter buds

text: SOLR

text: Eschscholzia californica

text: EcoRI

text: flower buds <= 2.5mm

characteristic: title

text: Phagemid

text: pBluescript SK (+/-)


text: College of Agronomy and Biotechnology, China Agricultural University

text: live

text: Generic

text: Cotton 3' RACE

text: Gossypium hirsutum

text: ovules of 0DPA

text: Cotton ovules of 0DPA


text: live

text: Generic

text: Cotton 5' RACE

text: Gossypium hirsutum

text: fiber of 18DPA

text: Cotton fiber of 18DPA


text: The Natural History Museum, London

text: live

text: Generic

text: pharate female

text: Mengenilla chobauti

text: female

text: Mengenilla

text: Plasmid

text: pCRII

characteristic: vector


text: The First Military Medical University

text: live

text: Generic

text: colon adenocarcinoma with different metastatic potentials

characteristic: cell type

text: sw620 and sw480

characteristic: cell-line

text: A differentially expressed gene subtractive library was constructed by using subtractive suppression hybridization between the pair of colon adenocarcinoma cell lines SW620 and SW480 with different metastatic potentials. These EST fragments were ligated with pGEM-T vectors and amplified in JM109 E. coli. After blue-white screening, the white positive inserts were picked out and stored.

text: JM109 E. coli

text: Homo sapiens

text: Homo sapiens colon carcinoma cell lines subtractive library

text: Plasmid

text: pGEM-T


characteristic: External Id

text: Commonwealth Scientific and Industrial Research Organisation Division of Plant Industry

text: live

text: Generic

text: DeltaEMERALD

characteristic: cultivar

text: mRNA was prepared from pooled root and hypocotyl tissues of the cotton cultivar DeltaEMERALD at different stages of Fov infection from 5hours to 7 days post infection. cDNA was synthesised from a NotI-oligodT primer/adapter using the manufacturers protocols (Life Technologies) and then ligated to a SalI adapter to facilitate directional cloning. The cDNA was cloned into the SalI and NotI sites of the Lambda ZIPLOX phage vector (Life Technologies). Constructed by Caitriona Dowd and Helen McFadden.

text: seedlings, pooled from 5hours to 7 days post infection with Fov.

text: Gossypium hirsutum

text: SalI

text: NotI

text: Fov infected root and hypocotyls tissues

text: Cotton Root and Hypocotyl tissues infected with Fusarium oxysporum f.sp. vasinfectum (Fov) Lambda ZIPLOX Library (CFUS)

text: Phage

text: Lambda ZIPLOX


characteristic: INSDC last update

text: live

text: Generic

text: DeltaEMERALD

text: mRNA was prepared from root and hypocotyl tissues of the cotton cultivar DeltaEMERALD. cDNA was synthesised from a NotI-oligodT primer/adapter using the manufacturers protocols (Life Technologies) and then ligated to a SalI adapter to facilitate directional cloning. The cDNA was cloned into the SalI and NotI sites of the Lambda ZIPLOX phage vector (Life Technologies). Constructed by Caitriona Dowd and Helen McFadden.

text: 5 day old seedlings

text: Gossypium hirsutum

text: SalI

text: NotI

text: Root and hypocotyl tissues

characteristic: tissue-type

text: Cotton Root and Hypocotyl Lambda ZIPLOX Library (CRH)

text: Phage

text: Lambda ZIPLOX


text: Tianjin Neurological Institute

text: live

text: Generic

text: brain

characteristic: organ

text: Rattus norvegicus

text: Wistar

characteristic: strain

text: abscess

text: wister rat brain abscess


text: US Department of Agriculture, Agriculture Research Service, Pacific West Area, Western Regional Research Center

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: F2 from 2-12-4-8-1-1-1-(1) x PI36909-12-811-(1)

text: Plants were grown in a growth chamber at the University of California, Davis (Akhunov). Premeiotic anthers were harvested, total RNA and poly(A) RNA were prepared, from each tissue and then pooled, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Akhunov, Chin, Choi, Close, Fenton, Kianian, Otto, Simons, Zhang) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

text: Premeiotic anthers

text: E. coli SOLR

characteristic: lab-host

text: Aegilops speltoides

text: EcoRI

text: XhoI

text: Anther

text: Aegilops speltoides wheat anther cDNA library

text: Phagemid

text: Lambda Uni-ZAP XR, excised phagemid


text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: The tissue, total RNA, and poly(A) RNA were prepared from apex at double-ridge stage to terminal-spikelet stage during transition from vegetative state to flower state, a cDNA library was made, and the cDNA clones were in vivo excised at the University of California, Davis (V. Echenique, B. Stamova, J. Dubcovsky). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

text: Seven week-old plants

text: E. coli XLOLR

text: Triticum monococcum

text: EcoRI

text: XhoI

text: Early reproductive apex

text: Triticum monococcum DV92 early reproductive apex cDNA library

text: Phagemid

text: Lambda Uni-ZAP XR, excised phagemid


text: live

text: Generic

text: Chinese Spring

text: Plants were grown under hydroponic conditions for four days. Root tips were excised and snap frozen (Ross and Gustafson) and total RNA was prepared at University of Missouri, Columbia. Poly(A) RNA was purified, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript SK(-)phagemids in the TJ Close lab (Chin and Close) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

characteristic: description

text: Four-day old seedling

characteristic: dev-stage

text: E. coli SOLR

text: Triticum aestivum

characteristic: organism

text: EcoRI

text: XhoI

text: Root tip at 1.0 to 1.5 mm stage

text: CS wheat unstressed root tip cDNA library

text: Phagemid

text: Lambda Uni-ZAP XR, excised phagemid


text: live

text: Generic

text: Plants were grown under hydroponic conditions, root tips were excised and snap frozen, total RNA was prepared at University of Missouri(Ross, Gustafson). Poly(A) RNA was purified, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript SK- phagemids in the TJ Close lab (Chin and Close) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

text: Seedling

text: E. coli SOLR

text: Triticum aestivum

text: EcoRI

text: XhoI

text: Root tip at 1.0 to 1.5 mm stage

text: CS wheat aluminum-stressed root tip cDNA library

characteristic: title

text: Phagemid pBluescript SK-

text: Lambda Uni-ZAP XR, excised phagemid

characteristic: vector


characteristic: External Id

text: live

text: Generic

text: Brevor

text: Plants were grown to seed maturity under conditions favoring seed dormancy (L. Dohery at K. Walker_Simmons lab, Washington State University, Pullman, WA). Embryos were cut from mature dormant seed (Doherty). Total RNA was prepared from these embryos, polyA was purified, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab at the University of California, Riverside (Chin, Fenton). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

text: Mature seed

text: E. coli SOLR

text: Triticum aestivum

text: EcoRI

text: XhoI

text: Seed embryo

text: Brevor wheat dormant embryo cDNA library

text: Phagemid

text: Lambda Uni-ZAP XR, excised phagemid


text: live

text: Generic

text: Chinese Spring

characteristic: cultivar

text: Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Roots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. The cDNA clones were in vivo excised to give pBluescript phagemids before normalization was carried out. The mass excision of phagemid library and normalization were done in HT Nguyen lab by D. Zhang at Texas Tech Univeristy. The normalization protocol used was modified from that of Soares'. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

text: Five day old seedling

text: E. coli DH10B

text: Triticum aestivum

text: EcoRI

text: XhoI

text: Etiolated shoot

text: Wheat etiolated seedling shoot normalized cDNA library

text: Phagemid

text: Lambda Uni-ZAP XR, excised phagemid pBluescript SK


characteristic: INSDC last update

text: live

text: Generic

text: Chinese Spring

text: Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Five-day old seedlings were transferred to 5 C cold room and kept for 48 hr. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. The cDNA clones were in vivo excised to give pBluescript phagemids before subtraction was carried out. The mass excision of phagemid library and subtraction were done in HT Nguyen lab by D. Zhang at Texas Tech Univeristy. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

text: Five-day old seedling

text: E. coli SOLR

text: Triticum aestivum

text: EcoRI

text: XhoI

text: Seedling

text: CS wheat cold-stressed seedling subtracted cDNA library

text: Phagemid

text: Lambda Uni-ZAP XR, excised phagemid


text: United States Department of Agriculture-Agriculture Research Service

text: live

text: Generic

text: Canadian horn flies exposed to diapause inducing conditions, followed by differential display. Bands cut from DD gel, eluted, reamplified, PCR products gel purified, ends polished and subcloned into vector. Reference notebook FG 14-30.

text: larvae and pupae under diapause inducing conditions

text: E. coli XL-10 Kan Gold

text: whole body

text: Haematobia irritans irritans

text: Srf I

text: mixed

characteristic: sex

text: Diff. display PCR-Script Amp lib. pg. 14-30

text: Phagemid

text: PCR-Script Amp


text: live

text: Generic

text: Canadian horn flies exposed to diapause inducing conditions, followed by differential display. Bands cut from DD gel, eluted, reamplified, PCR products gel purified, ends polished and subcloned into vector. Reference notebook FG 14-71.

text: E. coli Epicurean XL1-Blue MRF' Kan

text: whole body

characteristic: organ

text: Haematobia irritans irritans

text: Srf I

text: mixed

text: Diff. display PCR-Script Amp lib. pg. 14-71

text: Phagemid

text: PCR-Script Amp


text: live

text: Generic

text: Clontech PCR Select protocol. Pyrethroid and diazinon resistant wild type horn flies from April 1997 collection at Camp Cooley subtracted with Kerrville citrated blood-fed lab susceptible strain unfed newly emerged adults.

text: adults of mixed ages

text: E. coli Nova Blue

text: whole body

text: Haematobia irritans irritans

text: Sma I

text: mixed

text: Camp Cooley 4/97 Wild Type

characteristic: strain

text: Horn Fly PCR Select Subtracted Camp Cooley Library pg 7-189

text: Phagemid


characteristic: INSDC center name

text: live

text: Generic

text: Pyrethroid and diazinon resistant wild type horn flies from April 1997 collection at Camp Cooley, PCR products from degenerate touchdown PCR gel purified, ends polished and subcloned into vector. Reference notebook FG 11-32.

text: adults of mixed ages

text: E. coli XL-10 Kan Gold

text: whole body

text: Haematobia irritans irritans

text: Srf I

text: mixed

text: Camp Cooley 4/97 Wild Type

text: Touchdown PCR for candidate resistance genes pg FG 11-32

text: Phagemid

text: PCR-Script Amp


text: The Natural History Museum, London

characteristic: INSDC first public

text: live

text: Generic

text: pharate female

text: Eoxenos laboulbenei

text: female

text: Eoxenos

text: Plasmid

text: pCRII


text: Stanford University School of Medicine

text: live

text: Generic

text: The mixed organ cDNA library was generated using the ZAP-cDNA method by Stratagene. First strand cDNA synthesis was primed with a a 50 bp linker primer containing an oligo dT sequence preceeded by a synthetic XhoI site. 5 prime adaptors were used containing an EcoR1 cohesive end. The finished cDNAs were inserted in to the ZAP express vector unidirectionally in the sense orientation with respect to the lacZ promoter of pBK-CMV. An amplified library was prepared from approximately 3 million primary clones in the lambda ZAP Express vector. In vivo excision was then used to generate individual pBK-CMV phagemid clones for EST sequencing.

text: adult

text: Gasterosteus aculeatus

text: EcoR1 (5' adaptor)

text: XhoI (3' linker primer)

text: mixed male and female

text: Salinas river, CA

text: heads and internal organs combined

characteristic: tissue-type

text: SHGC-CDA

text: phagemid

text: lambda ZAP Express/pBK-CMV


text: Simon Fraser University

text: live

characteristic: INSDC status

text: Generic

text: Library Creator: Siemon Ng, Darren Bounds, Diana Palmquist, and Gurbir Dhadwal (Simon Fraser University). Sockeye salmon tissue contributors: Dr. Larry J Albright (Simon Fraser University)

text: Oncorhynchus nerka

text: Oncorhynchus nerka head

text: pDNR-LIB


text: Simon Fraser University

text: live

text: Generic

characteristic: NCBI submission model

text: Oncorhynchus nerka

text: Oncorhynchus nerka whole

text: pDNR-LIB


text: National Cancer Institute / NIH

text: live

text: Generic

characteristic: NCBI submission package

text: Library is oligo-dT primed and directionally cloned. PBMC - Peripheral Blood Mononuclear Cells. RNA was pooled from 3/6hour stimulation with PMA adn Ionomycin. 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.69 kb (range 0.70-5.0 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA). Note: this is a NIH_MGC Library.

text: DH10B (T1 phage-resistant)

characteristic: lab-host

text: Homo sapiens

text: SfiI (ggccattatggcc)

text: SfiI (ggccgcctcggcc)

text: Pooled

text: plasmid (ampicillin-resistant)

text: pDNR-LIB


text: Super Shrimp Inc.

text: live

text: Generic

text: The cDNA was isolated by random sequencing of cDNA clones from a white spot virus infected shrimp (Penaeus stylirostris) hepatopancreas cDNA library.

characteristic: description

text: Penaeus stylirostris

text: Hepatopancreas

text: Penaeus stylirostris white spot virus infected hepatopancreas


text: Rutgers University

text: live

text: Generic

text: oyster exposed to Perkinsus contamination

text: adult

characteristic: dev-stage

text: Crassostrea virginica

characteristic: organism

text: hemocytes and gill

text: subtracted Libraries from eastern oyster exposed to Perkinsus


text: Johns Hopkins University

text: live

text: Generic

text: Embryonic Day 18 post-fertilization

text: eye

text: Gallus gallus

text: White Leghorn chick

text: retina

text: Embryonic Day 18 (E18) Chick retina library

characteristic: title

text: plasmid

characteristic: vector


characteristic: External Id

text: Auburn University

text: live

text: Generic

text: lymph node

text: Sus scrofa

text: Pig endotoxin differential display library


text: National Cancer Institute / NIH

characteristic: INSDC last update

text: live

text: Generic

text: Clones from this library have been PCR-amplified using gene-specific primers to contain the complete open reading frame (based on known gene sequences available from NCBI's RefSeq). Template for PCR is cDNA derived from either pooled cytoplasmic polyA RNA from 30 cells lines or pooled total RNA from 10 different tissues (from BD Biosciences/Clontech and Washington University). PCR products are directionally cloned into the loxP sites of the pDNR-Dual vector. Library constructed by Dr. Narayan Bhat, Earl Bere III and Hongling Liao (Gene Expression Laboratory, Research Technology Program, SAIC Frederick, NCI-Frederick, Frederick, MD 21702). For information on which gene each clone represents, please visit our anonymous ftp site at ftp://image.llnl.gov/image/rearrayed_plates/IRBK.preSV.data Note: this is a NIH_MGC Library.

text: DH5A (T1 phage-resistant)

text: Homo sapiens

text: loxP-SalI

text: loxP-HindIII

text: mixed

text: plasmid (ampicillin-resistant)

text: pDNR-Dual


text: Northeast Forestry University

text: live

text: Generic

text: Tamarix androssowii

text: leaf

text: Tamarix androssowii suppression subtractive hybridization cDNA library


text: Washington University School of Medicine

text: live

text: Generic

text: 1st strand of cDNA was synthesized with reverse transcriptase and oligo(dT) beads, then cDNA was amplified by PCR using modified SMART primers. The final cDNA was cloned in pAMP1 vector in annealing reaction with Uracil DNA Glycosylase (UDG). Library constructed by Y.Korshunova and M. Lovett. Library materials provided by J. Gordon lab.

text: Mus musculus

text: colon epithelia progenitor cells

text: Colon epithelia progenitors cDNA

text: bacterial


text: live

text: Generic

text: The cDNA library was constructed by Keliang Tang, and Robert Cole at Washington University. cDNA was synthesized from poly mRNA using an oligo-dT primer containing a XhoI site. Following second strand synthesis, EcoRI adapters were ligated to the cDNA, and products were size-selected on sephacryl S500. The cDNA were directionally cloned into the EcoRI/XhoI prepared pBluescript II SK+ vector, and electroporated into ElectroTen Blue cells (Stratagene). The library may contain a small percentage of host or bacterial contaminants.

text: Tachyzoite

text: DH10B ElectroTen Blue cells(Stratagene)

text: Neospora caninum

text: EcoRI

text: XhoI

text: Nc-1 Tachyzoite cDNA Library

text: plasmid

text: pBluescript II SK+


text: live

text: Generic

text: The cDNA library was constructed by Keliang Tang, and Robert Cole at Washington University. PolyA mRNA was converted to cDNA using the template-switching PCR method (SMART cDNA, Clontech Inc.,). First strand was reverse transcribed using the CDS III/3' primer and a 5' template switch primer (Smart IV primer). The product of the first strand synthesis was PCR amplified using the same primer set and the fragments were digested with SfiI. The fragments were size selected, ligated into a modified pBluescript vector (Michael White, Montana State University) containing directional SfiI sites, and transformed into Electroten cells (Stratagene). Vector: SfiI sites were added to the multiple cloning region of pBluescript SK+ between the BamHI/EcoRI sites. The modified polylinker has the following sequence: 5'GAATTCGGCCATTACGGCC(G)n-- insert-- GGCCGCCTCGGCCCACGGATCC3'where n=3-4 G nucleotides.

text: ElectroTen cells (Stratagene)

text: Neospora hughesi

text: Neospora EN-1 cDNA Library 2

text: plasmid

text: Modified pBluescript (pBS SK+)


text: National Institute on Aging/National Institutes of Health

text: live

text: Generic

text: Mouse cDNA project by the Laboratory of Genetics, National Institute on Aging (NIA), Intramural Research Program, NIH (http://lgsun.grc.nia.nih.gov/cDNA). This is a long-transcript enriched cDNA library (Ref. Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]). Total RNAs were obtained from Dr.Mark G. Carter (NIH/NIA-IRP). EG cells were cultured at 37. C, 5% CO2 in DMEM supplemented with 15% ES cell-qualified FBS, 0.1mM non-essential amino acids, 2 mM glutamine, penicillin/streptomycin, 1 mM sodium pyruvate, 0.1 mM beta-mercaptoethanol, and 10000000 units of LIF per liter. Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 2.5 ug of total RNA, treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-S. The products were purified by phenol/chloroform and Centricon 100. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pCMV-SPORT6 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 4.0 kb. The library was constructed by Yulan Piao.

text: embryonic day 8

text: Mus musculus

text: SalI

text: NotI

text: male

characteristic: sex

text: NIA Mouse Embryonic Germ Cell cDNA Library (Long)

text: plasmid (ampicillin resistant)

text: pCMV-SPORT6 (Invitrogen)


text: live

text: Generic

text: Mouse cDNA project by the Laboratory of Genetics, National Institute on Aging (NIA), Intramural Research Program, NIH (http://lgsun.grc.nia.nih.gov/cDNA). This is a long-transcript enriched cDNA library (Ref. Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]).Total RNAs were extracted from a pool of 16 embryos at 9.5-days postcoitum. Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from6.1 ug of total RNA, treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-S. The products were purified by phenol/chloroform and Centricon 100. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pCMV-SPORT6 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 3.0kb. The library was constructed by Yulan Piao.

text: whole embryo including extraembryonic tissues at 9.5-days postcoitum

text: Mus musculus

text: SalI

text: NotI

text: E9.5 whole embryo

text: NIA Mouse E9.5 Whole Embryo cDNA Library (Long)

text: plasmid (ampicillin resistant)

text: pCMV-SPORT6 (Invitrogen)


text: live

text: Generic

text: Mouse cDNA project by the Laboratory of Genetics, National Institute on Aging (NIA), Intramural Research Program, NIH (http://lgsun.grc.nia.nih.gov/cDNA). This is a long-transcript enriched cDNA library (Ref. Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]). Total RNAs were extracted from a pool of 7 embryos at 6.5-days postcoitum. Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from0.53 ug of total RNA, treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-S. The products were purified by phenol/chloroform and Centricon 100. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pCMV-SPORT6 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 2.3kb. The library was constructed by Yulan Piao.

text: whole embryo including extraembryonic tissues at 6.5-days postcoitum

text: Mus musculus

text: SalI

text: NotI

text: E6.5 whole embryo

text: NIA Mouse E6.5 Whole Embryo cDNA Library (Long)

text: plasmid (ampicillin resistant)

text: pCMV-SPORT6 (Invitrogen)


text: Johns Hopkins University

text: live

text: Generic

text: neuron

characteristic: cell type

text: mRNA was purified using Trizol and poly-dT column and cDNA library was prepared from rat cortical neurons.

text: embryonic day 16 and days in vitro 2

text: brain

characteristic: organ

text: Rattus norvegicus

text: male, female

text: Sprague-Dawley

text: cortex

text: Rat cortical cDNA Library (in vitro 2 days)

text: plasmid


text: West Virginia University

characteristic: INSDC center name

text: live

text: Generic

text: ovary

text: Oncorhynchus mykiss

text: EcoRI

text: XhoI

text: Rainbow Trout Ovary Uni-Zap XR Vector library

text: pBluescript SK(+/-)


text: Nanjing Military Medical Institute

characteristic: INSDC first public

text: live

text: Generic

text: microvessel endothelium

text: week 17 post-birth

text: Rattus norvegicus

text: male

text: Sprague-Dawley

characteristic: strain

text: brain cortex

text: Rat brain microvessel cDNA Library


text: live

characteristic: INSDC status

text: Generic

text: The library was constructed by Claire Murphy and Dr. James McCarter at Washington University, St. Louis. The cDNA was made by using Dynabead oligo-dT priming (Dynal). PCR based library using a modified protocol from the SMART PCR cDNA Synthesis Kit from Clontech. Directionally cloned into the UDG sites of pAMP1. Adult nematodes were collected from infected dogs and provided by Dr. Prema Arasu of North Carolina State University, Raleigh,NC (Prema_Arasu@ncsu.edu). Normalization was performed using amplified ssDNA in re-association kinetics reaction to hybridize to the non amplified single stranded library.

text: adult

text: Toxocara canis

text: NotI

text: SalI

text: Toxocara canis pAMP1 v1 normalized

text: plasmid (ampicillin resistant)

text: pAMP1 (Gibco)


text: MPI fuer Zuechtungsforschung

text: live

text: Generic

characteristic: NCBI submission model

text: Antirrhinum majus

text: whole plant

characteristic: tissue-type

text: Antirrhinum majus whole plant


text: Michigan State University

text: live

text: Generic

characteristic: NCBI submission package

text: Porcine (Pig) brain library includes pre-frontal cortex, frontal cortex, hippocampus, hypothalamus, parietal cortex, amygdala, cerebellum, spinal cord, eye, accumbens, and fetal pig CNS

text: fetal, 10-day, 21-day, 5-week, mature boar, post-pubertal gilt, lactating sow

text: Sus scrofa

text: male and female

text: Brain and central nervous system

text: Porcine Brain Library


text: MIPS

text: live

text: Generic

text: cDNA-collection

characteristic: description

text: adult

characteristic: lab-host

text: Homo sapiens

text: SfiIA

text: SfiIB

text: 781 (synonym: hlcc4)


text: MIPS

text: live

text: Generic

text: fetal

characteristic: dev-stage

text: Homo sapiens

characteristic: organism

text: SfiIA

text: SfiIB

text: liver

text: 779 (synonym: hncc1)


text: Department of Botany & Plant Sciences, University of California

text: live

text: Generic

text: Pomeroy OP

characteristic: cultivar

text: Plants were grown in the greenhouse at University of California, Riverside. The scion was a open-pollinated (very probably selfed) seedling of Poncirus trifoliata cv Pomeroy that was selected as homozygous for the Ctv resistance gene. The rootstock was sweet orange infected with citrus tristeza virus (CTV) isolate T514 over 1 year before sampling (CTV infects sweet orange, but not genotypes carrying the Ctv resistance gene. Shoots 10-30 cm long were harvested in October 2000, and the green phloem (bark) was removed and frozen quickly in dry ice. Total RNA was extracted using TriZol reagent (Gibco). Poly(A) RNA was purified, a cDNA library was made, and 0.5 million primary lambda cDNA clones were in vivo excised to give a population of pBluescript SK(-) phagemids. All steps to this point were performed in the ML Roose lab at the University of California, Riverside by X. Ye. Phagemids were plated, plasmid DNA purified, cDNA clones archived, and DNA sequences determined bi-directionally using an ABI3730 at the University of California Riverside Genomics Institute, Core Instrumentation Facility, (Choi, Kingan). Chromatogram files were transmitted to UC Riverside (by Choi), then processed at UC Riverside (by Wanamaker) using the HarvEST pipeline (http://harvest.ucr.edu) to remove vector and cloning oligo sequences and various contaminants, and to trim to a high quality region. Sequences that retained a phred 17 region of at least 100 bases were deposited to GenBank.

text: 10 - 30 cm shoots

text: E. coli TJC121

text: Citrus trifoliata

text: EcoRI

text: XhoI

text: Phloem

text: Poncirus trifoliata CTV-challenged cDNA library - UCR

characteristic: title

text: Phagemid pBluescript SK(-)

text: Lambda Uni-ZAP XR, excised phagemid

characteristic: vector


characteristic: External Id

characteristic: INSDC last update

text: live

text: Generic

text: Pomeroy OP

text: Plants were grown in the greenhouse at University of California, Riverside. The scion was a open-pollinated (very probably selfed) seedling of Poncirus trifoliata cv Pomeroy that was selected as homozygous for the Ctv resistance gene. The rootstock was sweet orange infected with citrus tristeza virus (CTV) isolate T514 over 1 year before sampling (CTV infects sweet orange, but not genotypes carrying the Ctv resistance gene. Shoots 10-30 cm long were harvested in October 2000, and the green phloem (bark) was removed and frozen quickly in dry ice. Total RNA was extracted using TriZol reagent (Gibco). Poly(A) RNA was purified, a cDNA library was made, and 0.5 million primary lambda cDNA clones were in vivo excised to give a population of pBluescript SK(-) phagemids. All steps to this point were performed in the ML Roose lab at the University of California, Riverside by X. Ye. Phagemids were plated, plasmid DNA purified, cDNA clones archived, and DNA sequences determined bi-directionally using an ABI3730 at the Arizona Genomics Institute, University of Arizona (Collura, Feuerbacher, Kim, Kudrna, Wing, Yu). Chromatogram files were transmitted to UC Riverside (by Yu), then processed at UC Riverside (by Wanamaker) using the HarvEST pipeline (http://harvest.ucr.edu) to remove vector and cloning oligo sequences and various contaminants, and to trim to a high quality region. Sequences that retained a phred 17 region of at least 100 bases were deposited to GenBank.

text: 10 - 30 cm shoots

text: E. coli TJC121

text: Citrus trifoliata

text: EcoRI

text: XhoI

text: Phloem

text: Poncirus trifoliata CTV-challenged cDNA library - AGI

text: Phagemid pBluescript SK(-)

text: Lambda Uni-ZAP XR, excised phagemid


text: Auburn University

text: live

text: Generic

text: brain

characteristic: organ

text: Sus scrofa

text: hypothalamus

text: Pig brain heat stress differential display library


text: Center for Tropical Disease Research and Training, University of Notre Dame

characteristic: INSDC center name

text: live

text: Generic

text: embryo, larvae, pupae, and adult

text: Anopheles funestus

text: male and female

characteristic: sex

text: West African

text: ESTs from wild-caught Anopheles funestus populations

text: Phagemid


text: Max Planck Institute of Molecular Genetics

characteristic: INSDC first public

text: live

text: Generic

text: 1st strand cDNA was prepared from mRNA obtained from brain material of two feti, 14.8 and 15.8 weeks old, with a Not I - oligo(dT) primer [5' GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT 3']. Double-stranded cDNA was ligated to Sal I adaptors, digested with Not I and cloned into the Not I and Sal I sites of the pQE30NST vector. This cDNA expression library represents a subset of clones that were selected for protein expression and has been normalised by oligonucleotide fingerprinting. The library was constructed by Konrad Bussow.

text: embryonic, 14.8 and 15.8 weeks (determined by foot length)

text: Homo sapiens

text: SalI

text: NotI

text: brain

text: hEx1 (RZPD no. 800)

text: Plasmid

text: pQE30NST (AF074376)


text: Shanghai Institute of Hematology, Ruijin Hospital Affiliated to Shanghai Second Medical University

text: live

characteristic: INSDC status

text: Generic

text: Total RNA was extracted from the kidney tissues of mature zebrafish. The poly (A)+ RNA fraction was separated from total RNA by oligo (dT) cellulose chromatography. Library was initially constructed in the lambdaZAP Express vector (Stratagene) and in vivo excised into pBS-CMV vector.

text: mature

text: kidney

text: Danio rerio

text: XhoI

text: EcoRI

text: Zebrafish Kidney Marrow cDNA library

text: plasmid

text: pBS-CMV


text: Washington University School of Medicine

text: live

text: Generic

characteristic: NCBI submission model

text: Original lib:1st strand of cDNA was synthesized with reverse transcriptase and oligo(dT) beads, then cDNA was amplified by PCR using modified SMART primers. The final cDNA was cloned in pAMP1 vector in annealing reaction with Uracil DNA Glycosylase (UDG). Normalized by generating biotin labeled linear PCR using original lib cut with Sal I as template, followed with Sau3A digest, and hybridizing with ssDNA from original lib. RNA provided by Jeff Gordon lab and library construction by Rose Tidwell and Mike Lovett.

text: XL1- Blue MRF'

text: Mus musculus

text: UDG cloning

text: UDG cloning

text: small intestnal epithelial progenitor cells

text: Normalized Small intestinal epithelia progenitors

text: phagemid (ampicillin resistant)


text: Incyte Genomics, Inc.

text: live

text: Generic

text: Homo sapiens

text: FLP

text: Plasmid

text: pDrive Cloning Vector


text: National Cancer Institute / NIH

text: live

text: Generic

characteristic: cell-line

text: This is a long-transcript enriched cDNA library (Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]) from WA01 cell line . Undifferentiated human ES cell line WA01/H1 was obtained from WiCell Research Institute, Inc., Madison, Wi, cultured according to their instructions, on MEF feeders. They formed round colonies with defined edges and were positive for alkaline phosphatase, SSEA-4, OCT3, OCT4, REX1, UTF, TERT, SOX2, CX43 and CX45. They are negative for GATA2, GATA4, PDX1, NCAM, MSX1, FLT3, SSEA-1, TUBB3, NES, GFAP, and EOMES. When confluent (18-10 days after plating), the ES cells from 4 X 6cm dishes were treated with 1 mg/ml collagenase, type IV (Invitrogen/GIBCO) for 5-10 min and gently scraped off with 5 ml pipette. RNA was purified with TRIzol Reagent from Invitrogen. Protocol ref: Genome Res. 11: 1553-1558 (2001). [PMID:11544199]) Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 3.4g of total RNA, treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform extraction, and separated from free linkers by Centricon-100 column. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-S for 25 cycles. The products were purified by phenol/chloroform extraction and Centricon-100 column. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pCMV-SPORT6 plasmid vector. The average insert size is about 3.6kb.

text: DH10B (T1 phage-resistant)

text: Homo sapiens

text: NotI

text: SalI

text: Embryonic Stem cells

characteristic: tissue-type

text: NIA Human H1 Embryonic Stem Cell cDNA Library (Long)

text: plasmid (ampicillin-resistant)


text: University of SFAX, National Institute for Engineering Studies

text: live

text: Generic

characteristic: NCBI submission package

text: Nicola

text: Vitroplants cultivated in the presence of 100 to 180 mM NaCl for ten days. RNAs were prepared and used in AFLP experiment. Salt induced cDNA were used for library construction.

characteristic: description

text: Ten days salt stressed leaves

text: Solanum tuberosum

text: Leaf

text: 100 mM NaCl salt stressed leaves

text: Plasmid

text: pMos blue vector


text: live

text: Generic

text: Nicola

text: Ten days salt stressed leaves

characteristic: dev-stage

text: Solanum tuberosum

text: Leaf

text: 180 mM NaCl salt stressed leaves

text: Plasmid

text: pMos blue vector


text: West Virginia University

text: live

text: Generic

text: Muscle

text: Oncorhynchus mykiss

characteristic: organism

text: EcoRI

text: XhoI

text: Rainbow Trout Muscle Uni-Zap XR Vector library

text: pBluescript SK(+/-)


text: Agriculture & Agri-Food Canada

text: live

text: Generic

text: Sclerotinia sclerotiorum (strain 100) was innoculated onto B. napus Zhongyou 821. A 1 cm section of stem epidermal tissue comprising the edge of the expanding lesion and adjacent tissue was collected and used to make the library. Fungal sequences were identified using BLASTX. Single pass sequencing was done using the T7 primer (TAATACGACTCACTATAGGG).

text: E. coli

characteristic: lab-host

text: Sclerotinia sclerotiorum

text: EcoRI

text: XhoI

characteristic: strain

text: mycelia

text: ZY821 stem SS infected

text: pBluescript SK-


text: Agriculture & Agri-Food Canada

text: live

text: Generic

text: Sclerotinia sclerotiorum (strain 100) was innoculated into minimal salts citrus pectin medium and allowed to grow for 10 days at room temperature prior to harvest. Single pass sequencing was done using the T3 (ATTAACCCTCACTAAAGGGA) and T7 primers (TAATACGACTCACTATAGGG).

text: E. coli

text: Sclerotinia sclerotiorum

text: EcoRI

text: XhoI

text: mycelia

text: SS pectin

characteristic: title

text: pBluescript SK-

characteristic: vector


characteristic: External Id

text: Auburn University

characteristic: INSDC last update

text: live

text: Generic

text: Gonad

text: Crassostrea virginica

text: NotI

text: SalI

text: Crassostrea virginica Gonad

text: Phagemid


text: Auburn University

characteristic: INSDC center name

text: live

text: Generic

text: Gill

characteristic: organ

text: Crassostrea virginica

text: NotI

text: SalI

text: Crassostrea virginica Gill

text: Phagemid