text: Agriculture and Agri-food Canada

text: live

text: Generic

text: Complete late stage buds (5 to 8 mm in size) were used for cDNA library construction. Tissue was collected by Dr. Kevin Rozwadowski and Tricia Kreiser. poly-A primed library using SuperScript Plasmid System with Gateway Technology for cDNA Synhesis and Cloning kit (Invitrogen). The sequence has been processed using Lucy.

text: Brassica napus

text: Not I

text: Sal I

text: Floral Buds

text: Brassica napus Late bud


status: PUBLIC

characteristic: External Id

text: Agriculture and Agri-food Canada

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: DH12075 (double haploid line from Cresor x Westar cross)

tag: attribute

characteristic: cultivar

text: The library was made from seedlings grown at room temperature in the dark for 10 days. poly-A primed using ZAP-cDNA Synthesis Kit (Stratagene). The sequence has been processed using Lucy.

characteristic: description

text: Brassica napus

characteristic: organism

text: Eco RI

text: Xho I

text: Etiolated seedlings

characteristic: tissue-type

text: Brassica napus Etiolated seedlings (Uni-ZAP)

characteristic: title

text: Uni-ZAP XR / pBluescript SK

characteristic: vector


text: Agriculture and Agri-food Canada

text: live

text: Generic

text: The library was made from seedlings grown at room temperature in the dark for 10 days. poly-A primed library using SuperScript Plasmid System with Gateway Technology for cDNA Synhesis and Cloning kit (Invitrogen). The sequence has been processed using Lucy.

text: Brassica napus

text: Not I

text: Sal I

text: Etiolated seedlings

text: Brassica napus Etiolated seedlings (pSPORT1)


text: Agriculture and Agri-food Canada

text: live

text: Generic

text: The library was made from cotyledons showing no damage from insect feeding (open field plot), before any leaves had grown. Seeding was done after a heavy infestation by flea beetles, about a week after samples for DC library were collected. poly-A primed library using SuperScript Plasmid System with Gateway Technology for cDNA Synhesis and Cloning kit (Invitrogen). The sequence has been processed using Lucy.

text: Brassica napus

text: Not I

text: Sal I

text: Cotyledon

text: Brassica napus Undamaged cotyledons


text: Agriculture and Agri-food Canada

text: live

text: Generic

text: The library was made from plants grown at 20oC days for 16 hours and 18oC nights until the 4-leaf stage at which point they were put at 4oC in the dark for 4 hours. poly-A primed library using SuperScript Plasmid System with Gateway Technology for cDNA Synhesis and Cloning kit (Invitrogen). The sequence has been processed using Lucy.

text: Brassica napus

text: Not I

text: Sal I

text: Leaves

text: Brassica napus Cold acclimation - dark

text: pSPORT1 (modified: GCGGCCGCCc*GACTAGTGAGCTC*cggacgcgtggGTCGAC)


text: Agriculture and Agri-food Canada

text: live

text: Generic

text: The library was made from plants grown at 20oC days for 16 hours and 18oC nights until the 4-leaf stage at which point they were put at 4oC for 4 hours. poly-A primed library using SuperScript Plasmid System with Gateway Technology for cDNA Synhesis and Cloning kit (Invitrogen). The sequence has been processed using Lucy.

text: Brassica napus

text: Not I

text: Sal I

text: Leaves

text: Brassica napus Cold acclimation - light


text: Agriculture and Agri-food Canada

text: live

text: Generic

text: Seeds from B. napus DH12075 line were germinated on PlantProd 6-11-31 plus 0.85g/L calcium nitrate with aeration. Plants were grown at 20oC days for 16 hours and 18oC nights until the 4-leaf stage at which point they were transferred to the same media containing 200mM mannitol for 4 hours. poly-A primed library using SuperScript Plasmid System with Gateway Technology for cDNA Synhesis and Cloning kit (Invitrogen). The sequence has been processed using Lucy.

text: Brassica napus

text: Not I

text: Sal I

text: Leaves

text: Brassica napus Leaf - drought

text: pSPORT2 / pBluescript II SK+


text: Agriculture and Agri-food Canada

text: live

text: Generic

text: poly-A primed library. The sequence has been processed using Lucy.

text: Brassica napus

text: Not I

text: Sal I

text: Root

text: Brassica napus Root - drought

text: pSPORT1 may have been modified


text: Agriculture and Agri-food Canada

text: live

text: Generic

text: The library was made from cotyledons damaged by insect feeding (open field plot), before any leaves had grown. poly-A primed library using SuperScript Plasmid System with Gateway Technology for cDNA Synhesis and Cloning kit (Invitrogen). The sequence has been processed using Lucy.

text: Brassica napus

text: Not I

text: Sal I

text: Cotyledon

text: Brassica napus Damaged cotyledons


text: CMBN, University of Oslo

text: live

text: Generic

text: adult

characteristic: dev-stage

text: Rattus norvegicus

text: female

characteristic: sex

text: Wistar

characteristic: strain

text: kidney

text: Rattus norvegicus 5' RACE Aquaporin-4 cDNA clones

text: pCR2.1-Topo (Invitrogen)


text: University of Aberdeen

text: live

text: Generic

text: Four day old cultures grown in 10% liquid V8 broth. Contact Pieter van West (p.vanwest.ac.uk) for further information

text: Four day old mycelia

text: Pythium oligandrum

text: EcoRI

text: NotI

text: CBS 530.74

text: Pythium oligandrum vegetative mycelia

text: pBS II sk+


text: University of Aberdeen

text: live

text: Generic

text: Three day old cultures interacting with heat-killed Phytophthora infestans. Contact Pieter van West (p.vanwest.ac.uk) for further information

text: Three day old sporulating mycelia

text: Phytophthora infestans (heat-killed)

characteristic: lab-host

text: Pythium oligandrum

text: EcoRI

text: NotI

text: CBS 530.74

text: P. oligandrum interaction with killed Phytophthora infestans

text: pBS II sk+


characteristic: External Id

text: Vegetable Research Center

text: live

text: Generic

text: BY

text: cDNA-AFLP analysis was carried out to dicover vernalization-related EST drived by low-temperature (3-5).

text: leaves

characteristic: organ

text: Brassica rapa

text: vernalization-related cDNA-AFLP


text: Institute of Science and Technology

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: stage 4(2 days after egg laying)

text: Gryllus bimaculatus

text: early embryos

text: Gryllus bimaculatus early embryos stage 4(2 days after egg laying)


text: Institute of Science and Technology

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: stage 6(3 days after egg laying)

text: Gryllus bimaculatus

text: early embryos

text: Gryllus bimaculatus early embryos stage 6(3 days after egg laying)


text: University of SFAX, National Institute for Engineering Studies

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Deglet Nour

text: DNA fragments isolated by ISSR-PCR from date palm male and or female flowers taken from the field and compared to hermaphrodite flowers obtained in vitro.

text: in vitro induced hermaphrodite flowers taken at different treatment stages or natural male flowers

text: Phoenix dactylifera

text: EcoRI

text: EcoRI

text: Flowers

text: Date palm flowers

text: Plasmid

text: pCR 2.1 vector


text: Northwest A&F University

text: live

text: Generic

text: Suwon 11

characteristic: cultivar

text: wheat leaves was inoculated with stripe rust fungus

characteristic: description

text: Triticum aestivum

text: leaf

text: Incompatible SSH cDNA library from wheat leaves inoculated by stripe rust fungus


text: The J. Craig Venter Institute

text: live

text: Generic

text: Total RNA isolated using TRIzol reagent. No DNaseI digestion was performed. Total RNAs were pooled together. Double strand cDNA were synthesized from pooled RNA using SMART technology (Clontech). The prepared cDNA was normalized by cDNA denaturation/reassociation, treatment by duplex-specific nuclease(DSN) and amplification of normalized fraction by PCR. The normalized cDNA was then digested with SfiI, size-fractioned, directionally ligated into pDNR-LIB (Clontech) and electroprated into GC10 competent cells (Gene Choice). Clones were sequenced from the 5' end only. RNA provided by Greg May. This library is also called MTYA.

text: Medicago truncatula

characteristic: organism

text: SfiIA

text: SfiIB

text: flowers, early seeds, late seeds and stems

characteristic: tissue-type

text: pDNR-LIB (Clontech)


text: The J. Craig Venter Institute

text: live

text: Generic

text: Williams 82

text: Total RNA isolated using TRIzol reagent. No DNaseI digestion was performed. Total RNAs were pooled together. Double strand cDNA were synthesized from pooled RNA using SMART technology (Clontech). The prepared cDNA was normalized by cDNA denaturation/reassociation, treatment by duplex-specific nuclease(DSN) and amplification of normalized fraction by PCR. The normalized cDNA was then digested with SfiI, size-fractioned, directionally ligated into pDNR-LIB (Clontech) and electroprated into GC10 competent cells (Gene Choice). Clones were sequenced from the 5' end only. RNAs provided by Marc Libault and Babu Valliyodan. This library is also referred to as GLLA.

text: Glycine max

text: SfiIA

text: SfiIB

text: stem, apical meristem, flowers, young pods, green seeds

characteristic: title

text: pDNR-LIB (Clontech)

characteristic: vector


text: The J. Craig Venter Institute

text: live

text: Generic

text: Williams 82

text: Total RNA isolated using TRIzol reagent. No DNaseI digestion was performed. Total RNAs were pooled together. Double strand cDNA were synthesized from pooled RNA using SMART technology (Clontech). The prepared cDNA was normalized by cDNA denaturation/reassociation, treatment by duplex-specific nuclease(DSN) and amplification of normalized fraction by PCR. The normalized cDNA was then digested with SfiI, size-fractioned, directionally ligated into pDNR-LIB (Clontech) and electroprated into GC10 competent cells (Gene Choice). Clones were sequenced from the 5' end only. RNAs provided by Steve Clough, Peter Lafayette, Babu Valliyodan, and Madan Bhattacharyya. This library is also called GLMA.

text: Glycine max

text: SfiIA

text: SfiIB

text: drought stressed and control stem, tissue culture suspension, salt stressed, drought stressed, and Pseudomonas infected leaves, etiolated seedlings

text: pDNR-LIB (Clontech)


text: The J. Craig Venter Institute

text: live

text: Generic

text: Williams 82

text: Total RNA isolated using TRIzol reagent. No DNaseI digestion was performed. Total RNAs were pooled together. Double strand cDNA were synthesized from pooled RNA using SMART technology (Clontech). The prepared cDNA was normalized by cDNA denaturation/reassociation, treatment by duplex-specific nuclease(DSN) and amplification of normalized fraction by PCR. The normalized cDNA was then digested with SfiI, size-fractioned, directionally ligated into pDNR-LIB (Clontech) and electroprated into GC10 competent cells (Gene Choice). Clones were sequenced from the 5' end only. RNAs provided by Marc Libault and Babu Valliyodan. This library is also called GLNA.

text: Glycine max

text: SfiIA

text: SfiIB

text: nodules, root tip, strip root, whole root, inoculated root, drought stressed root, salt stressed root, waterlogged root

text: pDNR-LIB (Clontech)


text: University of Florida

text: live

text: Generic

text: Juvenile

characteristic: dev-stage

text: Tritonia tetraquetra

text: CNS

text: Central Nervous System (CNS) from one juvenile Tritonia diomedea


text: Station Biologique de Roscoff

text: live

text: Generic

text: Suppression subtraction hybridisation (SSH) library corresponding to a differential screen between cDNA from immature sporophytes and immature gametophytes of the brown alga Ectocarpus siliculosus. Immature means that the algae had not yet produced reproductive structures. The SSH was carried out using the PCR-select kit (Clontech) following the recommended protocol except that an additional enrichment step (mirror orientation selection or MOS) was carried out (Rebrikov et al., 2000 Nucleic Acids Research 28: N20 e90). The cDNA fragments produced by the SSH protocol were cloned into the vector pCR2.1-TOPO.

text: young gametophyte

text: Ectocarpus siliculosus

text: male

characteristic: sex

text: Ec 32

characteristic: strain

text: Esil gametophyte SSH


characteristic: External Id

text: Station Biologique de Roscoff

text: live

text: Generic

text: young sporophyte

text: Ectocarpus siliculosus

text: male

text: Ec 32

text: Esil sporophyte SSH


text: Centro de Investigacion Sobre Enfermedades Infeccionsas, Instituto Nacional de Salud Publica

text: live

text: Generic

text: Anopheles albimanus female midgut (unfed) cDNA library.

text: Adult (3 days post-emergence)

text: Midgut

characteristic: organ

text: Anopheles albimanus

text: Female

text: white stripe

text: Plasmid

text: pBSKS


text: Rosenstiel School of Marine and Atmospheric Science - University of Miami

characteristic: INSDC center name

text: live

text: Generic

text: Heart cDNA from nine populations exposed to hypoxia, cold, heat, low and high salinity and hydrocarbons.

text: Fundulus heteroclitus

text: heart

text: Fundulus heteroclitus 151


characteristic: INSDC first public

text: live

text: Generic

text: Liver cDNAs from nine populations exposed to hypoxia, cold, heat, low and high salinity and hydrocarbons.

text: Fundulus heteroclitus

text: liver

text: Fundulus heteroclitus 152


characteristic: INSDC last update

text: live

text: Generic

text: All developmental stages (whole animal).

text: Fundulus heteroclitus

text: whole animal

text: Fundulus heteroclitus 153


text: live

characteristic: INSDC status

text: Generic

text: BBandE, brain, bone and eye from nine populations exposed to hypoxia, cold, heat, low and high salinity and hydrocarbons.

text: Fundulus heteroclitus

text: brain, bone and eye

text: Fundulus heteroclitus 154


text: live

text: Generic

characteristic: NCBI submission model

text: Internal organs (spleen, gall bladder, kidneys, fat) from nine populations exposed to hypoxia, cold, heat, low and high salinity and hydrocarbons.

text: Fundulus heteroclitus

text: spleen, gall bladder, kidneys, fat

text: Fundulus heteroclitus 155


text: live

text: Generic

characteristic: NCBI submission package

text: S.FandM, skin, fin and muscle from nine populations exposed to hypoxia, cold, heat, low and high salinity and hydrocarbons.

text: Fundulus heteroclitus

text: skin, fin and muscle

text: Fundulus heteroclitus 156


text: live

text: Generic

text: G-I tract and fatty tissue cDNAs from nine populations expose to hypoxia, cold, heat, low and high salinity and hydrocarbons.

text: Fundulus heteroclitus

text: G-I tract and fatty tissue

text: Fundulus heteroclitus 157


text: live

text: Generic

text: Gill cDNAs from nine populations expose to hypoxia, cold, heat, low and high salinity and hydrocarbons.

text: Fundulus heteroclitus

text: gill

text: Fundulus heteroclitus 158


text: live

text: Generic

text: Mixed developmental, tissues and stressors from nine populations: all developmental stages (whole animal), brains, liver, heart, muscles, spleen, eye, GI, fat, gill, bone, gall bladder and epithelia expose to hypoxia, cold, heat, low and high salinity and hydrocarbons.

text: mixed

text: Fundulus heteroclitus

text: mixed

text: Fundulus heteroclitus 159


text: Victorian AgriBiosciences Centre, Primary Industries Research Victoria

text: live

text: Generic

text: Surpass 400

characteristic: cultivar

text: Clontech SMART cDNA cloned into pGEMt.

text: 7 days old

text: Brassica napus

text: Cotyledon

text: 12na Brassica napus Cotyledon 7 days old infected with Leptosphaeria maculans

text: plasmid

text: pGEMT Easy


text: live

text: Generic

text: Oscar

text: Clontech SMART cDNA cloned into pGEMt.

characteristic: description

text: Young, not fully expanded

text: Brassica napus

text: Leaves

text: 03na Brassica napus young leaves, not fully expanded

text: plasmid

text: pGEMt Easy


text: live

text: Generic

text: Surpass 400

text: Clontech SMART cDNA cloned into pGEMt.

text: 14 days old

text: Brassica napus

characteristic: organism

text: Leaves

text: 14na Brassica napus 14 day old Leaves, infected with Leptosphaeria maculans

text: plasmid

text: pGEMt Easy


text: live

text: Generic

text: BLaze

text: Clontech SMART cDNA cloned into pGEMt.

text: immature

text: Brassica juncea

text: pods

characteristic: tissue-type

text: 01ju Brassica juncea immature pods

text: plasmid

text: pGEMt Easy


text: live

text: Generic

text: BLaze

text: Clontech SMART cDNA cloned into pGEMt.

text: fully expanded

text: Brassica juncea

text: leaves

text: 08ju Brassica juncea Old leaves infected with Leptosphaeria maculans

characteristic: title

text: plasmid

text: pGEMt Easy


text: live

text: Generic

text: Clontech SMART cDNA cloned into pGEMt.

text: young leaves not fully expanded

text: Brassica nigra

text: leaves

text: 03ni Brassica nigra young leaves, not fully expanded

text: plasmid

text: pGEMt Easy

characteristic: vector


text: National Cancer Institute / NIH

text: live

text: Generic

text: cDNA was primed using oligo-dT primer: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3' and cloned into the EcoRV/NotI sites of pExpress-1. Size-selection >1.4kb resulted in an average insert size of 2.1kb. This is a non-normalized primary library (normalized library is NIH_MGC_421) and was constructed by Express Genomics (Frederick, MD) for the (http://mgc.nci.nih.gov/) Mammalian Gene Collection.

text: DH10B TonA

characteristic: lab-host

text: heart

text: Rattus norvegicus

text: EcoRV

text: NotI

text: combined sample from 36 day 12 embryos/10 pregnant females

text: plasmid


text: National Cancer Institute / NIH

text: live

text: Generic

text: cDNA was primed using oligo-dT primer: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3' and cloned into the EcoRV/NotI sites of pExpress-1. Size-selection >1.4kb resulted in an average insert size of 2.0kb. This is a non-normalized primary library (normalized library is NIH_MGC_419) and was constructed by Express Genomics (Frederick, MD) for the (http://mgc.nci.nih.gov/) Mammalian Gene Collection.

text: DH10B TonA

text: kidney

text: Rattus norvegicus

text: EcoRV

text: NotI

text: combined sample from 60 day 13 embryos/17 pregnant females

text: plasmid


text: Agricultural research organization, Newe Yaiar research center

text: live

text: Generic

text: Cucumis melo L. cv. 'Arava' and Cucurbita moschata (F1 Hybrids,Cucurbita moschata Duchense x Cucurbita moschata Duchense)

text: Melon grafted on pumpkin. Suppression Subtractive Hybridization (SSH) libraries were developed from the grafted seedlings. The grafting was performed between melon and pumpkin. The sample of the grafting seedlings was taken from the grafting union zone and from a similar zone from the non-grafted seedlings. Subtractions were performed between the grafted seedlings vs. non-grafted scion or rootstock seedlings in direct and reciprocal procedures, on day 14 post grafting.

text: Fourteen days post grafting

characteristic: dev-stage

text: E. coli JM109 competent cells

text: Cucumis melo x Cucurbita moschata

text: Grafting union zone

text: Grafting union zone SSH Library

text: Plasmid


text: live

text: Generic

text: Melon grafted on pumpkin. Suppression Subtractive Hybridization (SSH) libraries were developed from the grafted seedlings. The grafting was perform between melon and pumpkin. The sample of the grafting seedlings was taken from the grafting union zone. Subtractions were perform between the grafted seedlings 4 and 14 days after grafting in direct and reciprocal procedures.

text: Four or Fourteen days post grafting

text: E. coli JM109 competent cells

text: Cucumis melo x Cucurbita moschata

text: Grafting union zone

text: Time post grafting SSH Library

text: Plasmid


text: USDA-ARS

text: live

text: Generic

text: Experiments were carried out on sugarbeet root maggot, Tetanops myopaeformis Röder, field-collected diapausing and matured third-instar larvae, that had been maintained in laboratory storage at 6+1oC for one, two, and five years. Larvae were collected from fields near St.Thomas (SE quarter of section 25, 160o N and range 53 W), in Pembina County, North Dakota. Larvae were dug from the soil, placed into plastic bags containing loose field soil, and brought to the laboratory where they were washed and transferred into plastic storage bags filled with moist silica sand. Insects in bags were then stored in an incubator at 6+1oC. The larvae were in pre-diapause at the time of collection from the field. Therefore we can be reasonably confident that the insects used for the 1-, 2-, and 5-year assessments had entered diapause soon after collection. Total RNA was extracted from field-collected (November 2004) diapausing larvae and larvae that had been stored in the laboratory for one, two, and five years using Trizol reagent. Differential display was carried out according to the manufacturer's protocol using GeneFishingTM DEG Premix kits (Seegene, Rockville, MD, USA). Fragments were cloned into pCR2.1 and sequenced, and primers were designed to synthesize probes. For northern blot analysis the concentrations of RNA samples were determined using a spectrophotometer and ethidium bromide was added to all samples to ensure equivalent loading. Total RNA (5 ug/sample) was separated on a 1% agarose denaturing gel (0.41M formaldehyde, 1X MOPS-EDTA-sodium acetate). At the end of each gel run, the resulting gel was photographed to compare rRNA intensity. The RNA was subsequently transferred onto a positively charged nylon membrane with downward capillary action using 20X SSC (i.e., 3 M NaCl, 0.3 M sodium citrate, pH 7.0) transfer buffer and were run overnight. The blots were air-dried and UV cross-linked at 12,000 uJ/cm2 and stored at -20oC. They were screened with digoxigenin[PC1]-labeled probes at a concentration of 20 ng/ml of hybridization buffer.

text: Matured third-instar larvae (diapausing and laboratory stored)

text: Tetanops myopaeformis

text: Differential display screening of dormant Tetanops myopaeformis

text: plasmid


text: Institute for Biochemistry, Faculty of Medicine

text: live

text: Generic

text: Library was prepared using Clontec PCR-Selct cDNA Subtraction Kit (BD Bioscience) starting from total RNA from intact limb and reamputated limb at day 4. We produced tester cDNA population of regenerated limbs which we hybridysed with driver cDNA population of intact tissue. By doing this we harvested down-regulated genes in regenerating tissue. Subtracted library of up-regulated genes was cloned into pCRII plasmids using TA cloning kit (Invitrogen).All the obtained clones were sequenced.

text: One Shot Competent Cells

text: limb

text: Ambystoma mexicanum

text: male

characteristic: sex

text: wild type

characteristic: strain

text: Axolotl limb subtracted library, downregulated, day 4

text: Plasmid

text: pCRII


characteristic: External Id

text: Institute of Himalyan Bioresource Technology (CSIR)

text: live

text: Generic

text: Arnebia euchroma

text: Callus

text: Subtractive cDNA libraries of Arnebia euchroma (Royle) Johnston

text: T/A CLONING pCR 2.1 (Invitrogen)


text: Institute for Plant Nutrition

characteristic: INSDC center name

text: live

text: Generic

text: TVu 91

text: Vigna unguiculata

text: leaf

text: SSH TVu 91


text: Institute for Plant Nutrition

characteristic: INSDC first public

text: live

text: Generic

text: TVu 1987

text: Vigna unguiculata

text: leaf

text: SSH TVu 1987


text: Chinese Academy of Sciences

characteristic: INSDC last update

text: live

text: Generic

text: Oryza rufipogon

text: Oryza rufipogon cv. W1943 library


text: Rowett Research Institute

text: live

characteristic: INSDC status

text: Generic

text: Isotricha intestinalis

text: Isotricha intestinalis library (McEwan NR)


text: Rowett Research Institute

text: live

text: Generic

characteristic: NCBI submission model

text: Entodinium simplex

text: Entodinium simplex library (McEwan NR)


text: Moredun Research Institute

text: live

text: Generic

characteristic: NCBI submission package

text: country: United Kingdom

text: Teladorsagia circumcincta

text: xL3 vs L4 SSH library


text: INRA

text: live

text: Generic

text: distribution : CRB GADIE, crb-gadie@jouy.inra.fr, INRA, CEA Radiobiologie et Etude du genome (LREG), Domaine de Vilvert, 78352, Jouy-en-Josas cedex, FRANCE, +33 (0) 1.34.65.29.14, +33 (0 ) 1.34.65.28.02.

text: morulae subtracted from blastocyst

text: Oryctolagus cuniculus

text: early embryo

text: SSH library morulae embryo subtracted from blastocyst embryo (lcao)

text: pGEMT Easy


text: INRA

text: live

text: Generic

text: early morulae substrated by 4 cell-stage embryo

text: Oryctolagus cuniculus

text: early embryo

text: SSH library 4 cells embryo subtracted from morulae embryo (part of lcaf library) (lcap)

text: pGEMT Easy


text: University of Copenhagen

text: live

text: Generic

text: brain

characteristic: organ

text: Neogale vison

text: brain

text: American mink brain


text: Korea Research Institute of Bioscience and Biotechnology

text: live

text: Generic

text: Physella acuta

text: whole body

text: 5' enriched Physa acuta whole body cDNA library


text: Kirin Brewery Co. Ltd

text: live

text: Generic

text: expressed during fermentation

characteristic: description

text: Saccharomyces pastorianus

text: normalized Saccharomyces pastorianus cDNA libraries


text: Hirosaki Industrial Research Institute

text: live

text: Generic

text: Fuji

characteristic: cultivar

text: mature

text: Malus domestica

characteristic: organism

text: fruit

text: Apple fruit cDNA library


text: Baylor College of Medicine

text: live

text: Generic

text: Library constructed by Jack Werren

text: Nasonia giraulti

text: NGP

characteristic: title

text: plasmid

text: pBluescriptSK(-)


text: Baylor College of Medicine

text: live

text: Generic

text: Library constructed by Jack Werren

text: Mixed-adult, pupa, larvae

text: Nasonia vitripennis

text: NVP

text: plasmid

text: pBluescriptSK(-)

characteristic: vector


text: Virginia State University

text: live

text: Generic

text: Goat pooled embryo and uterine tissues from d4 to d8 of pregnancy. Embryonic stages represented are 8-cell, morula, blastocyst, and hatched blastocyst.

text: embryonic day 4-8 post fertilization

text: Capra hircus

text: female

text: embryo, uterus (d4-8 pregnancy)

characteristic: tissue-type

text: Goat early pregnancy (d4-8) cDNA library

text: pAGEN


text: University of Geneva

text: live

text: Generic

text: Gymnophrys cometa

text: Gymnophrys cometa cDNA library


text: University of Geneva

text: live

text: Generic

text: Quinqueloculina sp.

text: Quinqueloculina sp. cDNA library


text: USDA-ARS

text: live

text: Generic

text: first instar larvae (3-days old)

characteristic: dev-stage

text: Mayetiola destructor

text: male and female

text: Great Plains, L, and Syrian flies

text: salivary glands

text: Hessian fly salivary gland cDNA Library


text: University of Manchester

text: live

text: Generic

text: Adult

text: Gill

text: Platichthys flesus

text: Wild type

text: Flounder gill library

text: pBluescript


text: University of Manchester

text: live

text: Generic

text: Adult

text: Gut

text: Platichthys flesus

text: Wild type

text: Flounder gut library

text: plasmid

text: pBluescript


text: University of Manchester

text: live

text: Generic

text: Platichthys flesus


text: University of South Carolina

text: live

text: Generic

text: Library made from placentas of 3 Peromyscus maniculatus bairdii, 2 Peromyscus polionotus subgriseus, and 1 from a hybrid between Peromyscus polionotus subgriseus and Peromyscus polionotus leucocephalus.

text: mature e16-18

text: SOLR strain of E.coli

characteristic: lab-host

text: placenta

text: Peromyscus mixed library

text: EcoRI or XbaI

text: XhoI

text: female

characteristic: sex

text: plasmid

text: pBluescript


characteristic: External Id

text: University of South Carolina

text: live

text: Generic

text: sexually mature virgin 6-month-old

text: E.coli

text: testis

text: Peromyscus maniculatus bairdii

text: EcoRI

text: XhoI

text: male

text: Washtenaw County, Michigan

characteristic: strain

text: plasmid

text: pBluescript


text: National Taiwan University

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: The subtractive cDNA library was constructed.

text: Taiwanofungus camphoratus

text: mycelia and basidiom

text: Antrodia cinnamomea mycelia and basidiom subtractive cDNA library

text: pGEM-T Easy


text: University of Agricultural Sciences

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Magnaporthe grisea fungal infected leaves.

text: leaf

text: Eleusine coracana

text: Fingermillet, Fermentas


text: IEW, Vrije Universiteit Amsterdam

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: A cadmium (cad) or phenanthrene (phe) enriched library

text: Folsomia candida

text: Parthenogenic

text: Berlin

text: whole body

text: Folsomia candida SSH cDNA library


text: IEW

text: live

text: Generic

text: Normalized cDNA library

characteristic: description

text: Mixed age

text: Folsomia candida

text: Parthenogenetic

text: Berlin

text: Whole body

text: Folsomia candida normalized cDNA library


text: the Chinese University of Hong Kong

text: live

text: Generic

text: cercaria

text: Schistosoma japonicum

text: SfiI A

text: SfiI B

text: hermaphrodite

text: Chinese strain

text: Schistosoma japonicum cercaria cDNA library

text: phagemid

text: lambda TriplEx2


text: Rutgers, the State University of New Jersey

text: live

text: Generic

text: Agrostis scabra

text: Agrostis scabra supression subtractive hybridization library


text: Montana State University

text: live

text: Generic

text: Cap-trapped full length library in lambda-GW; Plant Journal 45 (847-856) 2006

text: 20 days after pollination; developmental stage IV

text: Seed

characteristic: organ

text: Ricinus communis

text: Endosperm

text: Castor seed endosperm

text: Phage

text: lambda GW


text: Chung-Ang University

text: live

text: Generic

text: Isolation of total RNA from the Clonorchis sinensis metacercaria

text: Metacercaria

text: Clonorchis sinensis

characteristic: organism

text: XhoI

text: EcoRI

text: CsMc

text: lambda vector


text: Lund University

text: live

text: Generic

text: The insects were kept at 23 C, 70 percent humidity and either a 18:6 or a 20:4 h Light:Dark cycle. Total RNA was extracted from a pool of 15 female abdomens without ovipositors, hence pheromone glands were excluded. The abdomen RNA was combined in equal amount with total RNA extracted from a pool of 24 insect bodies without abdomens and wings. The insects were collected 0-3 days post emergence, at every fourth hour of the day in order to get a broad representation of different transcripts present in the insect. The cDNA library was constructed using the SMART cDNA Construction Kit (BD Biosciences) according to the manufacturers' protocol. Full length cDNAs were directionally ligated into (lambda) TriplEx2 arms after trimming by SfiI. The lambda library was converted into a plasmid library in a Cre+ b/w strain (E. coli BNN132). Plasmid clones were randomly collected and used as template for PCR amplification of inserts using the plasmid-specific primers P104 and P105, followed by DNA sequencing using the forward primer P104.

text: Adults 0-3 days post emergence

text: Escherichia coli BNN132

text: Agrotis segetum

text: SfiI

text: SfiI

text: Female

text: As12, Biolog. Bundesanstalt f. Land u. Fort., Darmstadt, Ger.

text: Entire insect body apart from wings and ovipositor (i.e. pheromone gland was excluded)

text: As12B - Agrotis segetum body library

characteristic: title


text: University of British Columbia

text: live

text: Generic

characteristic: cultivar

text: Needles from 4 mature trees were harvested in the early fall (September). Equamolar amounts of mRNA from each treed was pooled for library construction. cDNA was prepared from 5 micrograms of mRNA and directionally ligated into the pBluescript II SK+ vector using Stratagenes pBluescript II XR cDNA Library Construction kit according to manufacturer's instructions, with modifications. Plasmid DNA was then transformed by electroporation into DH10B cells (Invitrogen). Clones used in sequencing were from the primary library, with an amplification step.

text: E. coli DH10B

text: Picea sitchensis

text: EcoRI (5' end of the cDNA)

text: XhoI (3' end of the cDNA)

text: hermaphrodite

text: Needles

text: Plasmid

text: pBluescript II SK+

characteristic: vector


text: University of British Columbia

text: live

text: Generic

text: Needles from 4 mature trees were harvested in the late fall (November). Equamolar amounts of mRNA from each tree was pooled for library construction. cDNA was prepared from 5 micrograms of mRNA and directionally ligated into the pBluescript II SK+ vector using Stratagenes pBluescript II XR cDNA Library Construction kit according to manufacturer's instructions, with modifications. Plasmid DNA was then transformed by electroporation into DH10B cells (Invitrogen).

text: E. coli DH10B

text: Picea sitchensis

text: EcoRI (5' end of the cDNA)

text: XhoI (3' end of the cDNA)

text: hermaphrodite

text: Needles

characteristic: tissue-type

text: Plasmid

text: pBluescript II SK+


text: J. Craig Venter Institute

text: live

text: Generic

text: Total RNA from whole seedling (with 1 set of true leaves), buds, and anthers were pooled together. Double strand cDNA were synthesized from pooled RNA using SMART technology (Clontech). Then the prepared cDNA was normalized by cDNA denaturation/reassociation, treatment by duplex-specific nuclease(DSN) and amplification of normalized fraction by PCR. The normalized cDNA was then digested with SfiI, fractioned, directionally ligated into pDNR-LIB (Clontech) and electroprated into GC10 competent cell (Gene Choice). Sequences were generated from 5' and 3' ends of clones. ~isolation_source: background of the beach~ ~country: Spain: Ris, Noja, Santander province~ ~lat-lon: 43.4911 N, 3.5298 W~

text: Raphanus raphanistrum subsp. maritimus

text: SfiIA

text: SfiIB

text: pDNR-LIB(Clontech)


text: University of Sassari

text: live

text: Generic

text: EST-fragment of a gene up-regulated during mycelial contact between Trichoderma harzianum T4.67 and the plant pathogen Rhizoctonia solani

text: Trichoderma lixii

text: mycoparasitism related gene


text: Lund University

text: live

text: Generic

text: fibroblast

characteristic: cell type

text: Sminthopsis crassicaudata

text: Sminthopsis fibroblast Library


text: National Cancer Institute / NIH

text: live

text: Generic

text: cDNA was primed using oligo-dT primer: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3' and cloned into the EcoRV/NotI sites of pExpress-1. Size-selection >1.4kb resulted in an average insert size of 2.5kb. This is a non-normalized primary library (normalized library is NIH_MGC_430) and was constructed by Express Genomics (Frederick, MD) for the (http://mgc.nci.nih.gov/) Mammalian Gene Collection.

text: DH10B TonA

characteristic: lab-host

text: lung

text: Rattus norvegicus

text: EcoRV

text: NotI

text: normal lung from day 12 embryos (82 pooled, from 18 females)

text: plasmid


characteristic: External Id

text: National Cancer Institute / NIH

text: live

text: Generic

text: cDNA was primed using oligo-dT primer: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3' and cloned into the EcoRV/NotI sites of pExpress-1. Size-selection >1.3kb resulted in an average insert size of 1.7kb. This is a non-normalized primary library (normalized library is NIH_MGC_432) and was constructed by Express Genomics (Frederick, MD) for the (http://mgc.nci.nih.gov/) Mammalian Gene Collection.

text: DH10B TonA

text: embryo

text: Rattus norvegicus

text: EcoRV

text: NotI

text: 135 pooled embryos from 33 pregnant females

text: plasmid


text: National Cancer Institute / NIH

text: live

text: Generic

text: cDNA was primed using oligo-dT primer: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3' and cloned into the EcoRV/NotI sites of pExpress-1. Size-selection >1.35kb resulted in an average insert size of 1.8kb. This is a non-normalized primary library (normalized library is NIH_MGC_434) and was constructed by Express Genomics (Frederick, MD) for the (http://mgc.nci.nih.gov/) Mammalian Gene Collection.

text: DH10B TonA

text: spleen

text: Rattus norvegicus

text: EcoRV

text: NotI

text: pooled spleens from 97 embryos from 28 pregnant females

text: plasmid


text: J. Craig Venter Institute

text: live

text: Generic

text: Total RNA from whole seedling (with 1 set of true leaves), buds, and anthers were pooled together. Double strand cDNA were synthesized from pooled RNA using SMART technology (Clontech). Then the prepared cDNA was normalized by cDNA denaturation/reassociation, treatment by duplex-specific nuclease(DSN) and amplification of normalized fraction by PCR. The normalized cDNA was then digested with SfiI, fractioned, directionally ligated into pDNR-LIB (Clontech) and electroprated into GC10 competent cell (Gene Choice). Sequences were generated from 5' and 3' ends of clones. ~isolation_source: border of a cereal field~ ~country: Spain: Colmenar Viejo, just N of Madrid~ ~lat-lon: 40.6 N, 3.7 W~

text: Raphanus raphanistrum subsp. raphanistrum

text: SfiIA

text: SfiIB

text: pDNR-LIB(Clontech)


text: J. Craig Venter Institute

text: live

text: Generic

text: Total RNA from whole seedling (with 1 set of true leaves), buds, and anthers were pooled together. Double strand cDNA were synthesized from pooled RNA using SMART technology (Clontech). Then the prepared cDNA was normalized by cDNA denaturation/reassociation, treatment by duplex-specific nuclease(DSN) and amplification of normalized fraction by PCR. The normalized cDNA was then digested with SfiI, fractioned, directionally ligated into pDNR-LIB (Clontech) and electroprated into GC10 competent cell (Gene Choice). Sequences were generated from 5' and 3' ends of clones. ~collection_date: 1989~ ~isolation_source: alfalfa field~ ~country: USA: Chenango Bridge, NY~ ~lat-lon: 42.1841 N, 75.8353 W~

text: SfiIA

text: SfiIB

text: pDNR-LIB(Clontech)


text: University of Technology of Vienna, Institute of Chemical Engineering

text: live

text: Generic

text: Trichoderma cf. koningiopsis T78

characteristic: strain

text: Trichoderma cf. koningiopsis T78 mycelium Lambda Zap Library L14


text: Department of Plant Biotechnology, College of Life Resource Sciences, Dongguk University

characteristic: INSDC center name

text: live

text: Generic

text: Jinpumkong, Dawonkong

text: Plants from which samples were collected at V5 stage after planting. V5 stage plant were exposed to a single does of 10 kJ/m2/day UV-B for 5 day of each h period.

text: Glycine max

text: leaves

text: Substract library of UV-B treated leaves in Glycine max


text: Temasek Lifesciences Laboratory

characteristic: INSDC first public

text: live

text: Generic

text: DNA was synthesized from 3-week-old zebarfish gonad total RNA using SMART cDNA library construction kit (Clontech). SfiI-digested and size fractionated cDNA fragments (on average 1-1.5 kb in length) were ligated into pBS-SK-Sfi vector (a pBluescript-SK based vector with Sfi I sites) and transformed into XL10-Gold competent cells. The insert from randomly selected white colonies was PCR-amplified using M13 forward and reverse primers and partially sequenced by M13 reverse primer (forward read) or by a anchored oligo-T primer (reverse read, _R).

characteristic: dev-stage

text: gonad

text: Danio rerio

text: mixed

characteristic: sex

text: To (Singaporean strain)

text: 3-week-old gonad (TLL)

text: plasmid

text: pBS-SK-Sfi


text: Temasek Lifesciences Laboratory

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: cDNA was synthesized from 4-week-old zebrafish gonad total RNA using SMART cDNA library construction kit (Clontech). SfiI-digested and size fractionated cDNA fragments (on average 1-1.5 kb in length) were ligated into pBS-SK-Sfi vector (a pBluescript-SK based vector with Sfi I sites) and transformed into XL10-Gold competent cells. The insert from randomly selected white colonies was PCR-amplified using M13 forward and reverse primers and partially sequenced by M13 reverse primer (forward read) or by a anchored oligo-T primer (reverse read, _R).

text: gonad

text: Danio rerio

text: mixed

text: Toh (Singaporean strain)

text: 4-week-old gonad (TLL)

text: plasmid

text: pBS-SK-Sfi


text: Temasek Lifesciences Laboratory

text: live

text: Generic

characteristic: NCBI submission package

text: cDNA was synthesized from 4-week old testis and 3-week-old gonad total RNA using SMART PCR cDNA synthesis kit (Clontech). PCR-SelectTM cDNA subtraction kit (Clontech) was used to enrich for fragments, which were present in the 4-week old testis but not in the 3-week-old gonad sample. To decrease the number of background clones, mirror oriented selection was applied as described by Rebrikov et al. (2000, NAR 28:20 e90). The selectively amplified cDNA fragments (on average 400-800bp in length) were ligated into pGEM-T (Promega) and transformed into XL10-Gold competent cells (Stratagene). The insert from randomly selected white colonies was PCR-amplified using M13 forward and reverse primers and partially sequenced by M13 reverse primer.

characteristic: description

text: gonad

characteristic: organ

text: Danio rerio

text: mainly males

text: Toh (Singaporean strain)

text: 4-week-old testis subtracted 1 (TLL)

text: pGEM-T


text: Temasek Lifesciences Laboratory

text: live

text: Generic

text: cDNA was synthesized from 4-week old testis and 4-week-old ovary total RNA using SMART PCR cDNA synthesis kit (Clontech). PCR-SelectTM cDNA subtraction kit (Clontech) was used to enrich for fragments, which were present in the 4-week old testis but not in the 4-week-old ovary sample. To decrease the number of background clones, mirror oriented selection was applied as described by Rebrikov et al. (2000, NAR 28:20 e90). The selectively amplified cDNA fragments (on average 400-800bp in length) were ligated into pGEM-T (Promega) and transformed into XL10-Gold competent cells (Stratagene). The insert from randomly selected white colonies was PCR-amplified using M13 forward and reverse primers and partially sequenced by M13 reverse primer.

text: gonad

text: Danio rerio

characteristic: organism

text: mainly males

text: Toh (Singaporean strain)

text: 4-week-old testis subtracted 2 (TLL)

characteristic: title

text: pGEM-T


text: J. Craig Venter Institute

text: live

text: Generic

text: Total RNA from whole seedling (with 1 set of true leaves), buds, and anthers were pooled together. Double strand cDNA were synthesized from pooled RNA using SMART technology (Clontech). Then the prepared cDNA was normalized by cDNA denaturation/reassociation, treatment by duplex-specific nuclease(DSN) and amplification of normalized fraction by PCR. The normalized cDNA was then digested with SfiI, fractioned, directionally ligated into pDNR-LIB (Clontech) and electroprated into GC10 competent cell (Gene Choice). Sequences were generated from 5' and 3' ends of clones. ~collection_date: May 2001~ ~isolation_source: dune natural reserve~ ~country: France: Port Barcares~ ~lat-lon: 42 48.690 N, 3 02.010 E~

text: Raphanus raphanistrum subsp. landra

text: SfiIA

text: SfiIB

characteristic: tissue-type

text: pDNR-LIB(Clontech)

characteristic: vector


text: Veterinarmedizinische Universitat Wien

text: live

text: Generic

text: Double-stranded cDNA was prepared from total RNA extracted from D. melanogaster females. This cDNA was digested with Mbo I, Nla III and Tai I. The 3' fragments produced by each restriction enzyme were recovered and pooled befored sequencing using the 454 technology.

text: Drosophila melanogaster

text: female

text: Dmel/genome stock

text: D. melanogaster - 454 sequencing (digested cDNA library)


text: Veterinarmedizinische Universitat Wien

text: live

text: Generic

text: Double-stranded cDNA was prepared from total RNA extracted from D. melanogaster females. This cDNA was nebulized and the 3' fragments were recovered and sequenced using the 454 sequencing technology.

text: Drosophila melanogaster

text: female

text: Dmel/genome stock

text: D. melanogaster - 454 sequencing (nebulized cDNA library)


text: University of Massachusetts, Amherst

text: live

text: Generic

text: Suspension culture cells

characteristic: cell type

characteristic: cell-line

text: Clones were generated by supression subtractive hybridization (SSH) using the Clontech PCR-Select cDNA Subtraction Kit with mirror orientation selection (MOS). Vector is pCR 2.1-TOPO. Tester sample was prepared from cells elicited with MJ for 6 hours. Driver sample was prepared from mock-elicited cells. The library should be over-represented for genes that are induced 6 hours following MJ elicitation.

text: Taxus cuspidata

text: Plasmid

text: pCR 2.1-TOPO


characteristic: External Id

text: University of Massachusetts, Amherst

text: live

text: Generic

text: Suspension culture cells

text: Clones were generated by supression subtractive hybridization (SSH) using the Clontech PCR-Select cDNA Subtraction Kit with mirror orientation selection (MOS). Vector is pCR 2.1-TOPO. Tester sample was prepared from cells elicited with MJ for 18 hours. Driver sample was prepared from mock-elicited cells. The library should be over-represented for genes that are induced 18 hours following MJ elicitation.

text: Taxus cuspidata

text: Plasmid

text: pCR 2.1-TOPO


text: University of Massachusetts, Amherst

characteristic: INSDC center name

text: live

text: Generic

text: Suspension culture cells

text: Clones were generated by supression subtractive hybridization (SSH) using the Clontech PCR-Select cDNA Subtraction Kit with mirror orientation selection (MOS). Vector is pCR 2.1-TOPO. Tester sample was prepared from cells elicited with MJ for 5 days. Driver sample was prepared from mock-elicited cells. The library should be over-represented for genes that are induced 5 days following MJ elicitation.

text: Taxus cuspidata

text: Plasmid

text: pCR 2.1-TOPO


text: University of Massachusetts, Amherst

characteristic: INSDC first public

text: live

text: Generic

text: Suspension culture cells

text: Clones were generated by supression subtractive hybridization (SSH) using the Clontech PCR-Select cDNA Subtraction Kit with mirror orientation selection (MOS). Vector is pCR 2.1-TOPO. Tester sample was prepared from mock-elicited cells. Driver sample was prepared from cells elicited with MJ for 6 hours. The library should be over-represented for genes that are down-regulated 6 hours following MJ elicitation.

text: Taxus cuspidata

text: Plasmid

text: pCR 2.1-TOPO


text: University of Massachusetts, Amherst

text: live

text: Generic

text: Suspension culture cells

text: Clones were generated by supression subtractive hybridization (SSH) using the Clontech PCR-Select cDNA Subtraction Kit with mirror orientation selection (MOS). Vector is pCR 2.1-TOPO. Tester sample was prepared from mock-elicited cells. Driver sample was prepared from cells elicited with MJ for 18 hours. The library should be over-represented for genes that are down-regulated 18 hours following MJ elicitation.

text: Taxus cuspidata

text: Plasmid

text: pCR 2.1-TOPO


text: University of Massachusetts, Amherst

text: live

text: Generic

text: Suspension culture cells

text: Clones were generated by supression subtractive hybridization (SSH) using the Clontech PCR-Select cDNA Subtraction Kit with mirror orientation selection (MOS). Vector is pCR 2.1-TOPO. Tester sample was prepared from mock-elicited cells. Driver sample was prepared from cells elicited with MJ for 5 days. The library should be over-represented for genes that are down-regulated 5 days following MJ elicitation.

text: Taxus cuspidata

text: Plasmid

text: pCR 2.1-TOPO


text: Temasek Lifesciences Laboratory

text: live

text: Generic

text: cDNA was synthesized from 5-week-old zebrafish gonad total RNA using SMART cDNA library construction kit (Clontech). SfiI-digested and size fractionated cDNA fragments (on average 1-1.5 kb in length) were ligated into pBS-SK-Sfi vector (a pBluescript-SK based vector with Sfi I sites) and transformed into XL10-Gold competent cells. The insert from randomly selected white colonies was PCR-amplified using M13 forward and reverse primers and partially sequenced by M13 reverse primer (forward read) or by a anchored oligo-T primer (reverse read, _R).

characteristic: dev-stage

text: gonad

text: Danio rerio

text: mainly males

text: Toh (Singaporean strain)

characteristic: strain

text: 5-week-old gonad (TLL)

text: plasmid

text: pBS-SK-Sfi


text: Temasek Lifesciences Laboratory

characteristic: INSDC last update

text: live

text: Generic

text: Zebrafish transgenic to vas::egfp were sorted based on their gonadal EGFP levels as transforming males and juvenile females at the age of 5 weeks post fertilization (Wang et al., 2007, J. Fish Biol. 70: 33-44). cDNA was synthesized from 5-week old transforming male gonad and 5-week-old ovary total RNA using SMART PCR cDNA synthesis kit (Clontech). PCR-SelectTM cDNA subtraction kit (Clontech) was used to enrich for fragments, which were present in the 5-week old transforming male gonad but not in the 5-week-old ovary sample. The selectively amplified cDNA fragments (on average 400-800bp in length) were ligated into pGEM-T (Promega) and transformed into XL10-Gold competent cells (Stratagene). The insert from randomly selected white colonies was PCR-amplified using M13 forward and reverse primers and partially sequenced by M13 reverse primer.

text: gonad

text: Danio rerio

text: mainly males

characteristic: sex

text: Toh (Singaporean strain)

text: 5-week-old transforming male gonad subtracted against 5-week-old ovary (TLL)

text: plasmid

text: pGEM-T


text: BC Cancer Agency

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: 8.5 months

text: ElectroMAX DH10B T1 Phage-Resistant Cells

characteristic: lab-host

text: kidney

text: Bos taurus

text: SalI (5' end of cDNA)

text: NotI (3' end of cDNA)

text: female

text: Hereford

text: kidney

text: pCMV SPORT 6.0


text: BC Cancer Agency

text: live

text: Generic

characteristic: NCBI submission package

text: 8.5 months

text: rumen

characteristic: organ

text: Bos taurus

text: SalI (5' end of cDNA)

text: NotI (3' end of cDNA)

text: female

text: Hereford

text: rumen

text: pCMV SPORT 6.0


text: The University of Tokyo

text: live

text: Generic

text: planula larvae

text: Acropora tenuis

characteristic: organism

text: Acropora tenuis (planula larvae) cDNA library

characteristic: title


text: University of Central Arkansas

text: live

text: Generic

text: Sequences produced from using RT-PCR using degenerate primers. specimen_voucher: PSU-SCA6

characteristic: description

text: Theobroma cacao

text: Theobroma cacao Degen Primers


text: Genoscope - Centre National de Sequencage

text: live

text: Generic

text: keratinocyte

characteristic: cell type

text: This sequence was obtained by production of EST minilibraries using the ORESTES method, from ex vivo normal human epidermis samples enriched in granular keratinocytes

text: Homo sapiens

text: epidermis

characteristic: tissue-type

text: Homo sapiens ORESTES from keratinocytes


characteristic: External Id

text: J. Craig Venter Institute (JCVI; www.jcvi.org)

text: live

text: Generic

text: Cells starved in 10mM Tris and harvested at 1, 2, 4.5 and 6 hours.

text: Tetrahymena thermophila

text: Tetrahymena thermophila SB210 cDNA library (starved)


text: University of Cambridge Department of Pathology

characteristic: INSDC center name

text: live

text: Generic

text: RT-PCR reactions were performed using a set of different primers tiled across the chimeric locus Orly. Each primer combination yielded a number of bands, which were cut and purified to generate this library. Individual bands were sequenced using appropriate primers.

text: adult (8 wk)

text: Mus musculus

text: male

text: testis

text: RT-PCR screen for variant splice forms of Orly


text: Medical College of Wisconsin

characteristic: INSDC first public

text: live

text: Generic

text: Chinchilla lanigera

text: Middle ear mucosa

text: Kerschner - Ehrlich non-normalized chinchilla middle ear mucosal cDNA library

text: Plasmid

text: pCRII

characteristic: vector


text: Third Institute of Oceanography, State Oceanic Administration (SOA)

text: live

text: Generic

text: E.coli

text: Ridgeia piscesae

text: Muscle

text: cDNA library of tubeworm, Ridgeia piscesae

text: Plasmid


text: The Horticulture and Food Research Institute of New Zealand Ltd

text: live

text: Generic

text: Library sequenced by Genesis Research & Development. Library made by Sean Marshall. Linker primer XhoI-poly(dT) ; Adapter EcoRI (AATTCGGCACGAGG)

text: Adult

characteristic: dev-stage

text: Epiphyas postvittana

text: EcoRI

text: XhoI

text: Antenna

text: (FAAA) Epiphyas postvittana male antennae

text: pBluescript SK(-)


characteristic: INSDC last update

text: live

text: Generic

text: Adult

text: Epiphyas postvittana

text: EcoRI

text: XhoI

text: Male antenna

text: (FAAB) Epiphyas postvittana male antennae

text: pBluescript SK(-)


text: live

text: Generic

text: 3rd instar larvae

text: Epiphyas postvittana

text: EcoRI

text: XhoI

text: Midgut

text: (FABA) Epiphyas postvittana 3rd instar larval midgut

text: pBluescript SK(-)


text: live

text: Generic

text: Library sequenced by Genesis Research & Development. Library made by Y. Yauk. Linker primer XhoI-poly(dT) ; Adapter EcoRI (AATTCGGCACGAGG)

text: 3rd, 4th and 5th instar

text: Epiphyas postvittana

text: EcoRI

text: XhoI

text: Midgut tissue

text: (FAEA) Lightbrown Apple Moth Larval Midgut

text: pBluescript SK(-)


text: live

text: Generic

text: Adult

text: Epiphyas postvittana

text: EcoRI

text: XhoI

text: Male antenna

text: (FAAD) Epiphyas postvittana male antennae

text: pBluescript SK(-)


text: Nagasaki University

text: live

text: Generic

text: Brachionus plicatilis

characteristic: strain

text: Brachionus plicatilis NH1L cDNA library

text: pBluescript SK(-)


text: Nagasaki University

text: live

characteristic: INSDC status

text: Generic

text: Brachionus plicatilis

text: NHIL

text: Brachionus plicatilis NHIL cDNA library

text: pBluescript SK(-)


text: Kanazawa University

text: live

text: Generic

characteristic: NCBI submission model

text: The differential display analysis were performed for isolating the microalgal genes of which transcriptions are induced under the iron deficient condition. The clone was isolated from the iron deficient cells of Prymnesium parvum. The clone name indicates one of genotypes classifying the clones obtained in the differential display analysis

text: stationary phase

text: Prymnesium parvum

text: iron deficient microalgal cell cDNA


text: Wageningen University

text: live

text: Generic

text: Blakeslea trispora

text: Blakeslea trispora mating-affected genes Library


text: Parco tecnologico Padano

text: live

text: Generic

text: Sus scrofa

text: muscle

text: Suppression subtractive Hybridization pig muscle


text: University of Rochester

text: live

text: Generic

text: cDNA library created from whole embryos aged 0-48 hours

text: 0-48 hours post laying

text: Tritia obsoleta

text: Ilyanassa obsoleta 0-48 hour cDNA library

text: phagemid

text: Bluescript II SK


text: J. Craig Venter Institute (JCVI; www.jcvi.org)

text: live

text: Generic

text: Cells grown to mid-log phase in Neff medium and separate equal aliquots treated with 11 mM CdCl2 or 500 mM CuSO4 for 1 h

text: Tetrahymena thermophila

text: Tetrahymena thermophila SB210 cDNA library (Heavy metals)


text: live

text: Generic

text: Equal aliquots of cells harvested from a mating between strains CU427 and CU428 at 3, 6, 9, and 12 h after mixing

text: Tetrahymena thermophila

text: Tetrahymena thermophila conjugation cDNA library


text: live

text: Generic

text: Cells grown for 10 hrs to mid-log phase in rich medium plus 800 nM Trichostatin A

text: Tetrahymena thermophila

text: Tetrahymena thermophila SB210 cDNA library (Trichostatin A)


text: live

text: Generic

text: Cells grown to mid-log phase in minimal defined medium

text: Tetrahymena thermophila

text: Tetrahymena thermophila SB210 cDNA library (minimal medium)


text: Seoul National University, Institute of Molecular Biology and Genetics

text: live

text: Generic

text: Nicotiana tabacum

text: flooding-treated Nicotiana tabacum cDNA library


text: Department of Chemistry and Biomolecular Sciences

text: live

text: Generic

text: A non-directional cDNA library was constructed in the plasmid vector pGEM-T (Promega) by T-ended cloning, according to the manufacturer's protocol. Total RNA was extracted from the adults of Dictyocaulus viviparus (strain HannoverDv2000) using the TriPure isolation reagent (Roche Molecular Biochemicals). Adult worms of D. viviparus were produced in helminth-free male Holstein-Friesian calves (five months of age). The worms were washed extensively (five times) in large volumes (100 ml) of fresh diethyl-pyrocarbonate (DEPC)-treated saline (22 oC), transferred to sterile, RNase-free screw-top cryovials (4 ml; Roth, Karlsruhe, Germany) and frozen as 200 ul pellets in a minimal amount of saline at -75 oC or -196 oC until RNA isolation. Integrity and yields of RNA were verified and estimated, respectively, using the Bioanalyzer 2100 (Agilent). Each RNA sample (~10 g) was treated with 2 U of DNase I (Promega), incubated at 37 oC for 30 min prior to heat denaturation of the enzyme (75 oC for 5 min) and then stored at -70oC until use. The cDNA was produced from 10 ug of total RNA from D. viviparus, using an oligo (dT) primer and Superscript II reverse transcriptase (Invitrogen) and then purified over DyeEx columns (Qiagen).

text: Dictyocaulus viviparus

text: Dictyocaulus viviparus HannoverDv2000 cDNA

text: Plasmid

text: pGEM-T


text: Texas A & M University-Kingsville

text: live

text: Generic

text: Clontech (cat. #634903) Creator SMART cDNA Library Construction Kit

text: adult

text: Agkistrodon piscivorus leucostoma

text: male

characteristic: sex

text: venom gland

text: Agkistrodon piscivorus leucostoma venom gland cDNA library


text: The Royal Veterinary and Agricultural University

text: live

text: Generic

characteristic: NCBI submission package

text: Amnionamn from KVL (Royal Veterinary and Agricultural University).

text: Amnion

text: Sus scrofa

text: amn


text: live

text: Generic

text: Aorta from DIAS (Danish Institute of Agricultural Sciences).

text: Aorta

characteristic: organ

text: Sus scrofa

text: aor


text: live

text: Generic

text: Bladder from DIAS (Danish Institute of Agricultural Sciences).

text: Bladder

text: Sus scrofa

characteristic: organism

text: bla


text: live

text: Generic

text: Cerebellum from DIAS (Danish Institute of Agricultural Sciences).

text: Cerebellum

text: Sus scrofa

text: cbe

characteristic: title


text: live

text: Generic

text: Blood from BGI (Beijing Genomics Institute).

characteristic: description

text: Blood

text: Sus scrofa

text: cbl


text: live

text: Generic

text: Brain from BGI (Beijing Genomics Institute).

text: Brain

text: Sus scrofa

text: cbr


text: live

text: Generic

text: Heart from DIAS (Danish Institute of Agricultural Sciences), RNA from BGI (Beijing Genomics Institute).

text: Heart

text: Sus scrofa

text: che


text: live

text: Generic

text: Jejunum from DIAS (Danish Institute of Agricultural Sciences), RNA from BGI (Beijing Genomics Institute).

text: Jejunum

text: Sus scrofa

text: cje


text: live

text: Generic

text: Kidney from DIAS (Danish Institute of Agricultural Sciences), RNA from BGI (Beijing Genomics Institute).

text: Kidney

text: Sus scrofa

text: cki


text: live

text: Generic

text: Liver from KVL (Royal Veterinary and Agricultural University), RNA from BGI (Beijing Genomics Institute).

text: Liver

text: Sus scrofa

text: cli


text: live

text: Generic

text: Lung from BGI (Beijing Genomics Institute)

text: Lung

text: Sus scrofa

text: clu


text: live

text: Generic

text: Lymph from KVL (Royal Veterinary and Agricultural University), RNA from BGI (Beijing Genomics Institute)

text: Lymph

text: Sus scrofa

text: cly


text: live

text: Generic

text: Ovary from KVL (Royal Veterinary and Agricultural University), RNA from BGI (Beijing Genomics Institute).

text: Ovary

text: Sus scrofa

text: cov


text: live

text: Generic

text: Skin from DIAS (Danish Institute of Agricultural Sciences), RNA from BGI (Beijing Genomics Institute).

text: Skin

text: Sus scrofa

text: csk


text: live

text: Generic

text: Spleen from KVL (Royal Veterinary and Agricultural University), RNA from BGI (Beijing Genomics Institute).

text: Spleen

text: Sus scrofa

text: csp


text: live

text: Generic

text: Stomach from DIAS (Danish Institute of Agricultural Sciences), RNA from BGI (Beijing Genomics Institute).

text: Stomach

text: Sus scrofa

text: cst


text: The Royal Veterinary and Agricultural University

text: live

text: Generic

text: Testicle from DIAS (Danish Institute of Agricultural Sciences), RNA from BGI (Beijing Genomics Institute).

text: Testicle

text: Sus scrofa

text: cte


text: live

text: Generic

text: Tendeloin from BGI (Beijing Genomics Institute)

text: Tendeloin

text: Sus scrofa

text: ctl


text: live

text: Generic

text: Thyroid from BGI (Beijing Genomics Institute)

text: Thyroid

text: Sus scrofa

text: cty


text: live

text: Generic

text: Uterus from DIAS (Danish Institute of Agricultural Sciences), RNA from BGI (Beijing Genomics Institute).

text: Uterus

text: Sus scrofa

text: cut


text: live

text: Generic

text: Foetus 50 days, brain stem from KVL (Royal Veterinary and Agricultural University)

text: Foetus 50 days

text: Brain stem

text: Sus scrofa

text: ebs


text: live

text: Generic

text: Foetus 50 days, cerebellum from KVL (Royal Veterinary and Agricultural University)

text: Foetus 50 days

text: Cerebellum

text: Sus scrofa

text: ece


text: live

text: Generic

text: Foetus 50 days, epidermis from KVL (Royal Veterinary and Agricultural University)

text: Foetus 50 days

text: Epidermis

text: Sus scrofa

text: eep


text: live

text: Generic

text: Foetus 50 days, jejunum from KVL (Royal Veterinary and Agricultural University)

text: Foetus 50 days

text: Jejunum

text: Sus scrofa

text: eje


text: live

text: Generic

text: Foetus 50 days, liver from KVL (Royal Veterinary and Agricultural University)

text: Foetus 50 days

text: Liver

text: Sus scrofa

text: eli


text: live

text: Generic

text: Foetus 50 days, lung from KVL (Royal Veterinary and Agricultural University)

text: Foetus 50 days

text: Lung

text: Sus scrofa

text: elu


text: live

text: Generic

text: Foetus 50 days, Eye from KVL (Royal Veterinary and Agricultural University)

text: Foetus 50 days

text: Eye

text: Sus scrofa

text: eye


text: live

text: Generic

text: Fatt from DIAS (Danish Institute of Agricultural Sciences)

text: Fatt

text: Sus scrofa

text: fat


text: live

text: Generic

text: Brain stem (107 days early born) from KVL (Royal Veterinary and Agricultural University)

text: 107 days early born

text: Brain stem

text: Sus scrofa

text: fbs


text: live

text: Generic

text: Foetus 100 days, cerebellum from KVL (Royal Veterinary and Agricultural University)

text: Foetus 100 days

text: Cerebellum

text: Sus scrofa

text: fce


text: live

text: Generic

text: Foetus 100 days, liver from KVL (Royal Veterinary and Agricultural University)

text: Foetus 100 days

text: Liver

text: Sus scrofa

text: fli


text: live

text: Generic

text: Foetus 100 days, thymus from KVL (Royal Veterinary and Agricultural University)

text: Foetus 100 days

text: Thymus

text: Sus scrofa

text: fty


text: The Royal Veterinary and Agricultural University

text: live

text: Generic

text: Gullet from DIAS (Danish Institute of Agricultural Sciences)

text: Gullet

text: Sus scrofa

text: gul


text: live

text: Generic

text: Heart from DIAS (Danish Institute of Agricultural Sciences)

text: Heart

text: Sus scrofa

text: hea


text: live

text: Generic

text: Kidney from DIAS (Danish Institute of Agricultural Sciences)

text: Kidney

text: Sus scrofa

text: kid


text: live

text: Generic

text: Liver from DIAS (Danish Institute of Agricultural Sciences)

text: Liver

text: Sus scrofa

text: liv


text: live

text: Generic

text: Lung from DIAS (Danish Institute of Agricultural Sciences)

text: Lung

text: Sus scrofa

text: lun


text: live

text: Generic

text: Mammae, collostrum producing from KVL (Royal Veterinary and Agricultural University)

text: collostrum producing

text: Mammae

text: Sus scrofa

text: mcp


text: live

text: Generic

text: Newborn 115 days, cartillage from KVL (Royal Veterinary and Agricultural University)

text: Newborn 115 days

text: Cartillage

text: Sus scrofa

text: nca


text: live

text: Generic

text: Newborn 115 days, colon from KVL (Royal Veterinary and Agricultural University)

text: Newborn 115 days

text: Colon

text: Sus scrofa

text: nco


text: live

text: Generic

text: Newborn 115 days, epidermis from KVL (Royal Veterinary and Agricultural University)

text: Newborn 115 days

text: Epidermis

text: Sus scrofa

text: nep


text: live

text: Generic

text: Newborn 115 days, jejunum from KVL (Royal Veterinary and Agricultural University)

text: Newborn 115 days

text: Jejunum

text: Sus scrofa

text: nje


text: live

text: Generic

text: Newborn 115 days, lung from KVL (Royal Veterinary and Agricultural University)

text: Newborn 115 days

text: Lung

text: Sus scrofa

text: nlu


text: live

text: Generic

text: Ovary from DIAS (Danish Institute of Agricultural Sciences)

text: Ovary

text: Sus scrofa

text: ova


text: live

text: Generic

text: Pancreas from DIAS (Danish Institute of Agricultural Sciences)

text: Pancreas

text: Sus scrofa

text: pan


text: live

text: Generic

text: Placenta from DIAS (Danish Institute of Agricultural Sciences)

text: Placenta

text: Sus scrofa

text: pla


text: live

text: Generic

text: Prostata from DIAS (Danish Institute of Agricultural Sciences)

text: Prostata

text: Sus scrofa

text: pro


text: live

text: Generic

text: Piglet 2 days, thymus from KVL (Royal Veterinary and Agricultural University)

text: Piglet 2 days

text: Thymus

text: Sus scrofa

text: pty


text: The Royal Veterinary and Agricultural University

text: live

text: Generic

text: Rectum from DIAS (Danish Institute of Agricultural Sciences)

text: Rectum

text: Sus scrofa

text: rec


text: live

text: Generic

text: Retina from DIAS (Danish Institute of Agricultural Sciences)

text: Retina

text: Sus scrofa

text: ret


text: live

text: Generic

text: Skin from DIAS (Danish Institute of Agricultural Sciences)

text: Skin

text: Sus scrofa

text: ski


text: live

text: Generic

text: Spleen from DIAS (Danish Institute of Agricultural Sciences)

text: Spleen

text: Sus scrofa

text: spl


text: live

text: Generic

text: Stomach from DIAS (Danish Institute of Agricultural Sciences)

text: Stomach

text: Sus scrofa

text: sto


text: live

text: Generic

text: Testes from DIAS (Danish Institute of Agricultural Sciences)

text: Testes

text: Sus scrofa

text: tes


text: live

text: Generic

text: Trachea from DIAS (Danish Institute of Agricultural Sciences)

text: Trachea

text: Sus scrofa

text: tra


text: live

text: Generic

text: Uterus from KVL (Royal Veterinary and Agricultural University)

text: Uterus

text: Sus scrofa

text: ute


text: University of Colorado Health Sciences Center

text: live

text: Generic

text: Differential display of mRNA technique was used to detect host gene expression changes during the drought stress induction. mRNA extracted from induced and not induced (control) 21 days soybean roots. To induce drought stress, roots was kept in dark without solution for 50, 100 and 150 min. Seventy-four differential display fragments were obtained by using DDRT-PCR. After RT-PCR analyses, three positive clones were eventually obtained. The DDRT-PCR fragments were cloned in the pGEM-T vector (Easy Vector System I, Promega) and sequenced.

text: day 21 post-germination

text: Glycine max

text: root

text: cDNA sequences from soybean roots induced by drought stress


text: University of Sao Paulo

text: live

text: Generic

text: embryonic cells

characteristic: cell type

characteristic: cell-line

text: directional cloned cDNA library. first strand synthesis with dT-attB2-Biotin primer. cDNAs > 350 bp selected by chromatography (Bio-Gel A50). double-stranded cDNAs ligated to attB1 adapter, size selected (> 350 bp) and cloned into pDONR-200 vector through recombination.

text: embryo

text: Rhipicephalus microplus

text: embryonic cells

text: Rhipicephalus (boophilus) microplus BME26 embryonic cell line library

text: Plasmid


characteristic: External Id

text: Hunan Agricultural University

text: live

text: Generic

text: Subtracted cDNA library prepared from intestinal tract of grass carp, Ctenopharyngodon idella

text: 1 year

text: E. coli DH5a

characteristic: lab-host

text: Ctenopharyngodon idella

text: Mixed male and female

text: Intestinal tract

characteristic: tissue-type

text: Subtraction library of intestinal tract

text: plasmid

text: pUCm-T


text: Kochi University

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: Lactuca sativa

text: Lactuca sativa library (Kiba A)


text: The Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences

characteristic: INSDC last update

text: live

text: Generic

text: adult

characteristic: dev-stage

text: Panax quinquefolius

text: Root

text: Panax quinquefolium Root cDNA Library

text: phagemid

text: pBluescriptII

characteristic: vector


text: Departement de Biochimie, Universite de Montreal

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Stachyamoeba lipophora

text: Stachyamoeba lipophora Homemade, polyA, Regular, urea (lib2_sl)


text: live

text: Generic

characteristic: NCBI submission package

text: Stachyamoeba lipophora

text: Stachyamoeba lipophora Homemade, polyA, Regular (lib1_sl)


text: North Dakota State University

text: live

text: Generic

text: Subtraction library created using ginseng (Panax quinquefolius) roots inoculated with Fusarium equiseti and uninoculated ginseng roots as tester and driver respectively.

text: Panax quinquefolius

text: root

text: Ginseng F. equiseti subtraction library

text: pGEM T-Easy


text: Yale University

text: live

text: Generic

text: Homo sapiens

text: Homo sapiens RACE PCR sequences


text: Washington University

text: live

text: Generic

text: Using genotype: y; cn bw sp

text: adult

text: Drosophila melanogaster

text: All

characteristic: sex

text: Whole Fly


text: Technion Israel Institute of Technology

text: live

text: Generic

text: Botrylloides leachii

characteristic: organism

text: Botrylloides leachi whole body regeneration


text: Australian Institute of Marine Science

text: live

text: Generic

text: libraries were prepared using the SMART cDNA Library Construction Kit (Clontech).

text: Penaeus vannamei

text: hemocytes and lymphoid organ

text: L. vannamei hemocytes and lymphoid organ library

characteristic: title

text: plasmid


text: J. Craig Venter Institute

text: live

text: Generic

text: Arena

characteristic: cultivar

text: Total RNA from whole seedling (with 1 set of true leaves), buds, and anthers were pooled together. Double strand cDNA were synthesized from pooled RNA using SMART technology (Clontech). Then the prepared cDNA was normalized by cDNA denaturation/reassociation, treatment by duplex-specific nuclease(DSN) and amplification of normalized fraction by PCR. The normalized cDNA was then digested with SfiI, fractioned, directionally ligated into pDNR-LIB (Clontech) and electroprated into GC10 competent cell (Gene Choice). Sequences were generated from 5' and 3' ends of clones.

characteristic: description

text: Raphanus sativus var. oleiformis

text: SfiIA

text: SfiIB

text: pDNR-LIB(Clontech)


text: Universidad Autonoma de Zacatecas

text: live

text: Generic

text: Guajillo

text: Library was prepared using the suppressive subtractive hybridization (SSH) methodology.

text: 7-day-old plantlets

text: Capsicum annuum

text: root

text: Capsicum annuum with binucleate Rhizoctonia Library

text: pGEM-T Easy


text: Washington University School of Medicine

text: live

text: Generic

text: Adult

text: Lucigen E.cloni 10G Supreme

text: Ancylostoma caninum

text: HincII

text: HincII

text: Female

text: Plasmid

text: pSMART


text: live

text: Generic

text: Adult

text: Lucigen E.cloni 10G Supreme

text: Ancylostoma caninum

text: HincII

text: HincII

text: Male

text: Plasmid

text: pSMART


text: British Antarctic Survey

text: live

text: Generic

text: Megaphorura arctica

text: Onychiurus arcticus control (lib 005)


text: British Antarctic Survey

text: live

text: Generic

text: Megaphorura arctica

text: Onychiurus arcticus dessicated -14 (lib 006)


text: British Antarctic Survey

text: live

text: Generic

text: Megaphorura arctica

text: Onychiurus arcticus recovering 8hr (lib 007)


text: British Antarctic Survey

text: live

text: Generic

text: Megaphorura arctica

text: Onychiurus arcticus recovered 24hr (lib 008)


text: British Antarctic Survey

text: live

text: Generic

text: Megaphorura arctica

text: Onychiurus arcticus dessicating -2 (lib 009)


text: University of Illinois at Urbana-Champaign

text: live

text: Generic

text: The library was constructed and normalized as described by Bonaldo, M.F., Lennon, G. and Soares, M.B. (1996), Genome Research 6(9): 791-806. Sequenced ST01 and ST02 clones from the normalized library were used for subtraction to obtain ST03, ST04 and ST05 clones.

text: head

characteristic: organ

text: Diabrotica virgifera virgifera

text: EcoRI(5' side of insert)

text: NotI (3' side of insert)

text: female

text: Normalized and subtracted western corn rootworm female head cDNA library

text: Plasmid


characteristic: External Id

text: USDA-ARS/Purdue University

text: live

text: Generic

text: First-instar larvae

text: Orseolia oryzae

text: Salivary glands

text: Asian rice gall midge salivary gland cDNA library


text: USDA-ARS/Purdue University

text: live

text: Generic

text: First-instar larvae

text: Sitodiplosis mosellana

text: Salivary glands

text: Orange wheat blossom midge salivary gland cDNA library


text: The Institute for Genomic Reserach

text: live

text: Generic

text: Total RNA isolated using TRIzol reagent. No DNaseI digestion was performed. Total RNAs were pooled together. Double strand cDNA were synthesized from pooled RNA using SMART technology (Clontech). The prepared cDNA was normalized by cDNA denaturation/reassociation, treatment by duplex-specific nuclease(DSN) and amplification of normalized fraction by PCR. The normalized cDNA was then digested with SfiI, size-fractioned, directionally ligated into pDNR-LIB (Clontech) and electroprated into GC10 competent cells (Gene Choice). Clones were sequenced from the 5' end only. RNA provided by Greg May.

text: Medicago truncatula

text: SfiIA

text: SfiIB

text: flowers, early seeds, late seeds and stems.

text: MTY

text: pDNR-LIB (Clontech)


text: Purdue University

text: live

text: Generic

text: cDNA library is made with Smart kit and Normalized by using duplex specific nuclease

text: egg, 1st, 2nd, 3rd instar and adult

text: Pediculus humanus capitis

text: NotI

text: NotI

text: mixed

text: BR and LR

characteristic: strain

text: Headlice composite library with all live stages combined

text: Plasmid

text: pBluescript II KS+


text: Zhejiang University

text: live

text: Generic

text: Jinchun No.4

text: Growing stage

text: E.coli DH5a

characteristic: lab-host

text: Cucumis sativus

text: EcoRI

text: Fruit

characteristic: tissue-type

text: Fruit cDNA library of cucumber (Cucumis sativus)

text: Plasmid

text: pGEM-T easy


text: Universite Paris-Sud

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: S.latifolia

characteristic: isolate

text: Microbotryum violaceum

text: Microbotryum violaceum 100.02

text: pGEM(R)-T


text: Microbial Ecology, Institution of Ecology

characteristic: INSDC last update

text: live

text: Generic

text: Betula pendula/Paxillus involutus mixed EST library

text: P104 (5'-GGGAAGCGCGCCATTGTGTT-3'

text: P105 (5'-AGTGAGCTCGAATTGCGGCC-3'

text: Mycorrhizal root tissue

text: DW1D (Mycorrhizal root tips - microcosm)

text: Plasmid

characteristic: vector


text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Paxillus involutus

text: SfiA

text: SfiA

text: Rhizomorphic tissue

text: DW1F (Rhizomorphs - microcosm)

text: Plasmid


text: live

text: Generic

characteristic: NCBI submission package

text: Paxillus involutus

text: SfiA

text: SfiA

text: Mycelium

text: MC1G (P starvation - liq. cult.)

text: Plasmid


text: live

text: Generic

text: Paxillus involutus

characteristic: organism

text: SfiA

text: SfiA

text: Mycelium

text: MC1H (Phosphate starvation+resupply - liq. cult.)

text: Plasmid


text: live

text: Generic

text: Paxillus involutus

text: SfiA

text: SfiA

text: Mycelium

text: MC1J (Nitrogen starvation - liq. cult.)

characteristic: title

text: Plasmid


text: live

text: Generic

text: Paxillus involutus

text: SfiA

text: SfiA

text: Mycelium

text: MCK (Nitrogen starvation+resupply - liq. cult.)

text: Plasmid


text: live

text: Generic

text: Paxillus involutus

text: Mycelium

text: MC_L (non-mycorrhizal (+Gln or NH4+) - petri dish)

text: Plasmid

text: pGEM-T


text: National Institute of Allergy and Infectious Diseases

text: live

text: Generic

text: Midgut

characteristic: cell type

text: PCR-based cDNA library made with SMART cDNA library construction kit from Clontech

text: Adult, blood fed

characteristic: dev-stage

text: Midgut

text: Lutzomyia longipalpis

text: Female

characteristic: sex

text: Jacobina

text: Midgut

text: Adult female Lutzomyia longipalpis midgut library blood fed

text: Phage

text: Lambda TriplEx2


characteristic: External Id

text: live

text: Generic

text: Midgut

characteristic: description

text: Adult, blood fed containing Leishmania chagasi

text: Midgut

characteristic: organ

text: Lutzomyia longipalpis

text: Female

text: Jacobina

text: Midgut

text: Adult female Lutzomyia longipalpis midgut library blood fed containing Leishmania chagasi

text: Phage

text: Lambda TriplEx2


text: live

text: Generic

text: Midgut

text: Adult, post blood meal digestion

text: Midgut

text: Lutzomyia longipalpis

text: Female

text: Jacobina

characteristic: strain

text: Midgut

characteristic: tissue-type

text: Adult female Lutzomyia longipalpis midgut library post blood meal digestion

text: Phage

text: Lambda TriplEx2


characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: Midgut

text: Adult, post blood meal digestion containing Leishmania chagasi

text: Midgut

text: Lutzomyia longipalpis

text: Female

text: Jacobina

text: Midgut

text: Adult female Lutzomyia longipalpis midgut library post blood meal digestion containing Leishmania chagasi

text: Phage

text: Lambda TriplEx2


characteristic: INSDC last update

text: live

text: Generic

text: Midgut

text: Adult, sugar fed

text: Midgut

text: Lutzomyia longipalpis

text: Female

text: Jacobina

text: Midgut

text: Adult female Lutzomyia longipalpis midgut library sugar fed

text: Phage

text: Lambda TriplEx2


text: Defence Agricultural Research Laboratory

text: live

text: Generic

text: Blue boy

characteristic: cultivar

text: Partial cDNA fragment obtained from cold stressed wheat leaf tissues by mRNA differential-display (DDRT-PCR) were cloned in PBSKS+ vector at EcoRV site.

text: seedlings (2 weeks old)

text: Triticum aestivum

text: Hermaphrodite

text: leaf

text: Winter wheat cold stressed leaf tissue

text: Plasmid

text: PBSKS+

characteristic: vector


text: Max Planck Institute Mol Plant Physiol

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: SNN

text: five days after germination

text: Nicotiana tabacum

text: seedling

text: seedling library, SL


text: Max Planck Institute Mol Plant Physiol

text: live

text: Generic

characteristic: NCBI submission package

text: SNN

text: harvested every 2 hours throughout diurnal cycle

text: 5 week old plants

text: Nicotiana tabacum

characteristic: organism

text: whole individual

text: DL, diurnal library


text: Max Planck Institute Mol Plant Physiol

text: live

text: Generic

text: SNN

text: kept at 4 degrees C overnight and then subjected to high light in the cold

text: 5 week old plants

text: Nicotiana tabacum

text: whole individual

text: COL, cold overnight library

characteristic: title


text: Max Planck Institute Mol Plant Physiol

text: live

text: Generic

text: SNN

text: Nicotiana tabacum

text: MSL, mixed source library


text: The Natural History Museum

text: live

text: Generic

text: Haemocytes

characteristic: cell type

text: An cDNA library, enriched for differentially expressed genes, was sequenced using M13F. Adaptor primer sequences were removed from sequence.

text: Biomphalaria glabrata

text: Hermaphrodite

text: NHM 1742

text: BgSSH (S-R) schistosome-exposed NHM 1742 Haemocytes

text: pGEM


text: The Natural History Museum

text: live

text: Generic

text: Haemocytes

text: Biomphalaria glabrata

text: Hermaphrodite

characteristic: sex

text: NHM 3017

text: BgSSH (R-S) schistosome-exposed NHM 3017 Haemocytes

text: pGEM


characteristic: External Id

text: The Natural History Museum

text: live

text: Generic

text: Haemocytes

text: Biomphalaria glabrata

text: Hermaphrodite

text: NHM 3017

text: BgSSH (Un-Ex) unexposed NHM 3017 Haemocytes

text: pGEM


text: The Natural History Museum

text: live

text: Generic

text: Haemocytes

characteristic: description

text: Biomphalaria glabrata

text: Hermaphrodite

text: NHM 3017

text: BgSSH (Ex-Un) schistosome-exposed NHM 3017 Haemocytes

text: pGEM


text: The Natural History Museum

text: live

text: Generic

text: Haemocytes

text: Biomphalaria glabrata

text: Hermaphrodite

text: NHM 3017

text: BgSSH (Un-Ex) unexposed NHM 3017 Haemocytes 2

text: pGEM


text: The Natural History Museum

text: live

text: Generic

text: Haemocytes

text: Biomphalaria glabrata

text: Hermaphrodite

text: NHM 3017

text: BgSSH (R-S) schistosome-exposed NHM 3017 Haemocytes 2

text: pGEM


text: The Natural History Museum

text: live

text: Generic

text: Haemocytes

text: Biomphalaria glabrata

text: Hermaphrodite

text: NHM 1742

text: BgSSH (S-R) schistosome-exposed NHM 1742 Haemocytes 2

text: pGEM


text: The Natural History Museum

text: live

text: Generic

text: Haemocytes

text: Biomphalaria glabrata

text: Hermaphrodite

text: NHM 3017

text: BgSSH (R-S) schistosome-exposed NHM 3017 Haemocytes 3

text: pGEM


text: The Natural History Museum

text: live

text: Generic

text: Haemocytes

text: Biomphalaria glabrata

text: Hermaphrodite

text: NHM 1742

text: BgSSH (S-R) schistosome-exposed NHM 1742 Haemocytes 3

text: pGEM


text: MPI for Molecular Genetics

text: live

text: Generic

text: wild population from ambient winter temperature of 3C subjected to temperature stress. lat_lon: 54.82 N 10.81 E. Total RNA was extracted from young leaf tissue and meristematic region of plants collected in wild by SCUBA at ambient temperature of 3C. In ambient seawater in the lab, temperature was increased over 2 days until 17C. Cleaned tissue frozen in liq N2 and stored at -80C prior to RNA extraction. Library construction was performed with Creator SMART cDNA library construction kit (Clontech). Initial LD-PCR amplification of 22 cycles for enrichment of full length transcripts was verified on Agarose gels. cDNA sizes (>500 base pairs) were selected using filter columns based on agarose gels as described in the Clontech manual.

text: adult

characteristic: dev-stage

text: Zostera marina

text: hermaphrodite

text: leaf


text: MPI for Molecular Genetics

text: live

text: Generic

text: wild population at ambient winter temperature of 3C subjected to temperature stress of 25C. lat_lon: 54.82 N 10.81 E. Total RNA was extracted from young leaf tissue and meristematic region of plants collected in wild by SCUBA at ambient temperature of 3C. In ambient seawater in the lab, temperature was increased over 3 days until 25C. Cleaned tissue frozen in liq N2 and stored at -80C prior to RNA extraction. Library construction was performed with Creator SMART cDNA library construction kit (Clontech. Initial LD PCR amplification of 22 cycles for enrichment of full length transcripts was verified on Agarose gels. cDNA sizes (>500 base pairs) were selected using filter columns based on agarose gels as described in the Clontech manual.

text: adult

text: Zostera marina

text: hermaphrodite

text: leaf


text: MPI for Molecular Genetics

text: live

text: Generic

text: six randomly chosen genotypes from a wild population at ambient winter temperature of 4C. lat_lon: 54.68 N 10 E. collection_date: Feb-2004. Total RNA was extracted from meristematic region of plants collected in wild by SCUBA at ambient temperature of 4C in February 2004. Subsequent storage of 1 day in ambient water of whole plants at 6C prior to library preparation. Cleaned tissue was shock frozen in liq N2 and stored at -80C prior to RNA extraction. Library construction was performed with Creator SMART cDNA library construction kit (Clontech). Initial PCR amplification of 18-25 cycles for enrichment of full length transcripts was verified on Agarose gels. cDNA sizes (>500 base pairs) were selected using filter columns based on agarose gels as described in the Clontech manual. Sequencing based on Plasmid preps using the primer GTA AAA CGA CGG CCA GT at Max-Planck-Institute for Limnology, Dept Ecophysiology, August-Thienemann-Str. 2, 24306 Ploen, Germany, Contact for clones: treusch@uni-muenster.de, Institut for Evolution and Biodiversity, University of Muenster, Huefferstr. 1, 48149 Muenster, Germany.

text: adult

text: Zostera marina

text: hermaphrodite

text: meristem


text: University of Muenster, Germany

text: live

text: Generic

text: six randomly chosen genotypes from a wild population at ambient winter temperature of 3C. lat_lon: 54.82 N 10.81 E. collection_date: Feb-2005. Total RNA was extracted from young leaf tissue and meristematic region of plants at ambient temperature of 3C (February 2005) collected in wild by SCUBA. The cleaned tissue was immediately shock frozen (<10min after collection) nd stored at -80C. Library construction was performed with Creator SMART cDNA library construction kit (Clontech). Initial LD-PCR amplification of 28 cycles for enrichment of full length transcripts was verified on Agarose gels. cDNA sizes (>500 base pairs) were selected using filter columns based on agarose gels as described in the Clontech manual. Only 50% of bacterial clones have insert, therefore we included an initial PCR based size selection prior sequencing. Sequencing based on Plasmid preps using the primer GTA AAA CGA CGG CCA GT at Max-Planck-Institute for Limnology, Dept Ecophysiology, August-Thienemann-Str. 2, 24306 Ploen, Germany Contact for clones: treusch@uni-muenster.de, Institut for Evolution and Biodiversity, University of Muenster, Huefferstr. 1, 48149 Muenster, Germany.

text: adult

text: Zostera marina

text: hermaphrodite

text: leaf


text: MPI for Molecular Genetics

text: live

text: Generic

text: six randomly chosen genotypes from a wild population at ambient summer temperature of 23C. lat_lon: 54.68 N 10 E. collection_date: 11-Aug-2004. Total RNA was extracted from meristematic region of plants collected in wild by SCUBA at ambient temperature of 23C (11 August 2004). Cleaned tissue was shock frozen in liq N2 prior to RNA extraction. Library construction was performed with Creator SMART cDNA library construction kit (Clontech). Initial LD-PCR amplification of 18-25 cycles for enrichment of full length transcripts was verified on Agarose gels. cDNA sizes (>500 base pairs) were selected using filter columns based on agarose gels as described in the Clontech manual. Sequencing based on Plasmid preps using the primer GTA AAA CGA CGG CCA GT at Max-Planck-Institute for Limnology, Dept Ecophysiology, August-Thienemann-Str. 2, 24306 Ploen, Germany, Contact for clones: treusch@uni-muenster.de, Institut for Evolution and Biodiversity, University of Muenster, Huefferstr. 1, 48149 Muenster, Germany.

text: adult

text: Zostera marina

text: hermaphrodite

text: meristem


text: Purdue University

text: live

text: Generic

text: Composed of non blood fed and various blood fed and replete whole I. scapularis and I. scapularis life-cycle stages.

text: Ixodes scapularis

text: Eco RV

text: Not I

text: Wikel

characteristic: strain

text: Ixodes scapularis Pooled Non-infected Normalized Library

text: Plasmid


text: National Institute of Agricultural Biotechnology (NIAB http://www.niab.go.kr/homepage/english/), Rural Development Administration (RDA http://www3.rda.go.kr/eng/)

text: live

text: Generic

text: Chiifu

text: Immature anthers isolated from floral buds of B. rapa plant were used for the total RNA extraction. The cDNA library was constructed using CreatorTM SMARTTM cDNA library construction kit (Clonetech, USA) according to the manufacturer's instructions and randomly selected cDNA clones were used for the 5-end sequence analysis.

text: immature anthers in floral bud

text: anther

characteristic: organ

text: Brassica rapa subsp. pekinensis

text: Sfi IA

text: Sfi IB

text: anther cDNA library KBAY

text: pDNR-LIB


characteristic: INSDC center name

text: live

text: Generic

text: Chiifu

text: RNA was extracted from the mixtures of callus including green callus and developing callus with or without shoot/root primodium on the MS agar media supplemented with ABA and BA. The cDNA library was constructed using CreatorTM SMARTTM cDNA Library Construction kit (Clontech, USA) according to the manufacturer's instructions and randomly selected cDNA clones were used for the 5-end sequence analysis.

text: callus under organogenesis stages on the tissue culture plate

text: callus

text: Brassica rapa subsp. pekinensis

text: Sfi IA

text: Sfi IB

text: callus cDNA library KBCG


characteristic: INSDC first public

text: live

text: Generic

text: Chiifu

text: Mature pollens isolated from the open flower of B. rapa were used for RNA extraction. The cDNA library was constructed using lambda ZAP-cDNA synthesis kit (Stratagene, USA) according to the manufacturer's instructions and randomly selected cDNA clones were used for the 5-end sequence analysis.

text: mature dried pollen

text: pollen

text: Brassica rapa subsp. pekinensis

text: Eco RI

text: Xho I

text: pollen cDNA library KBPL

text: pBluescriptII SK(-)


characteristic: INSDC last update

text: live

text: Generic

text: Chiifu

text: RNA was extracted from the 1-week-old seedlings grown on the MS salt media under fluorescent light. The cDNA library was constructed using CreatorTM SMARTTM cDNA Library Construction kit (Clontech, USA) according to the manufacturer's instructions and randomly selected cDNA clones were used for the 5-end sequence analysis.

text: 1-week-old seedling

text: seedling

text: Brassica rapa subsp. pekinensis

text: Sfi IA

text: Sfi IB

text: seedling cDNA library KBPS


text: live

text: Generic

text: Chiifu

characteristic: cultivar

text: RNA was extracted from the roots of 1-month-old B. rapa plants. The cDNA library was constructed using CreatorTM SMARTTM cDNA Library Construction kit (Clontech, USA) according to the manufacturer's instructions and randomly selected cDNA clones were used for the 5-end sequence analysis.

text: roots of 1-month-old plant

text: root

text: Brassica rapa subsp. pekinensis

text: Sfi IA

text: Sfi IB

text: root cDNA library KBRT


text: live

text: Generic

text: Chiifu

text: Siliques of 1-day- to 10-days-old after artificial pollination were used for RNA extraction. The cDNA library was constructed using CreatorTM SMARTTM cDNA Library Construction kit (Clonetech, USA) according to the manufacturer's instructions and randomly selected cDNA clones were used for the 5-end sequence analysis.

text: silique of 1-day- to 10-days-old after pollination

text: silique

text: Brassica rapa subsp. pekinensis

text: Sfi IA

text: Sfi IB

text: silique cDNA library KBSP

text: pDNR-LIB

characteristic: vector


text: live

characteristic: INSDC status

text: Generic

text: Chiifu

text: Siliques of 10-day- to 25-days-old after artificial pollination were used for RNA extraction. The cDNA library was constructed using CreatorTM SMARTTM cDNA Library Construction kit (Clonetech, USA) according to the manufacturer's instructions and randomly selected cDNA clones were used for the 5-end sequence analysis.

text: silique of 10-day- to 25-days-old after pollination

text: silique

text: Brassica rapa subsp. pekinensis

text: Sfi IA

text: Sfi IB

text: silique cDNA library KBSQ

text: pDNR-LIB


text: live

text: Generic

characteristic: NCBI submission model

text: Chiifu

text: Pistils were collected from floral buds. Total RNA was isolated from the pistil and used for mRNA preparation.

text: Pistils collected from floral buds

text: pistil

text: Brassica rapa subsp. pekinensis

text: Eco RI

text: Xho I

text: pistil cDNA library KCOV

text: pBluescriptII SK(-)


text: live

text: Generic

characteristic: NCBI submission package

text: Chiifu

text: Pistils in 5- to 10-days after pollination stages were collected from the flower. Total RNA was isolated from the pistil and used for mRNA preparation.

text: mixtures of pistils in 5- to 10-days after pollination

text: pistil

text: Brassica rapa subsp. pekinensis

text: Eco RI

text: Xho I

text: pistil cDNA library KCOW

text: pBluescriptII SK(-)


text: live

text: Generic

text: Chiifu

text: The lower parts of the main veins of the 2nd and 3rd leaves of 3-weeks-old plants were infected with Pectobacterium carotovora subsp. carotovora under controlled environment for the disease development. The leaf sections with no-visible disease symptoms from the 6hr-, 12hr-, and 24hr-post-innoculation were used for the RNA extraction. The SfiI digested double-stranded cDNAs were inserted into DraI digested pCNS-D2 vector and cDNA library was constructed using full-length enrichment methods (www.corebio.com).

text: the 2nd and 3rd leaves from the 3-weeks-old B. rapa plants

text: leaf

text: Brassica rapa subsp. pekinensis

characteristic: organism

text: Dra 1A

text: Dra 1B

text: infected leaf cDNA library KFPC


text: live

text: Generic

text: Chiifu

text: Root samples from the 1-week-, 3-weeks-, and 7-weeks-old B. rapa plant were used for the RNA extraction. The SfiI digested double-stranded cDNAs were inserted into DraI site of pCNS-D2 vector and cDNA library was constructed using full-length enrichment methods (www.corebio.com).

text: roots with mixed developmental stages of 1-week-, 3-weeks-, and 7-weeks-old B. rapa plant

text: root

text: Brassica rapa subsp. pekinensis

text: Dra 1A

text: Dra 1B

text: root cDNA library KFRT

characteristic: title


text: National Institute of Agricultural Biotechnology (NIAB http://www.niab.go.kr/homepage/english/), Rural Development Administration (RDA http://www3.rda.go.kr/eng/)

text: live

text: Generic

text: Chiifu

text: Poly (A+)-mRNA was isolated from total RNA using paramagnetic oligo (dT) beads (PolyAtract mRNA isolationII, Promega) according to the manufacturer's procedures. The cDNAs were cloned into lambda ZAPII (Stratagene) and clones were randomly selected for end sequence verification.

text: Cotyledon in greening process

text: Cotyledon

text: Brassica rapa subsp. pekinensis

text: EcoRI

text: XhoI

text: cotyledon cDNA library KHCT

text: pBluescriptII SK(-)


text: live

text: Generic

text: Chiifu

text: Defected mature leaf

text: Leaf

text: Brassica rapa subsp. pekinensis

text: EcoRI

text: XhoI

text: defected leaf cDNA library KHLD

text: pBluescriptII SK(-)


text: live

text: Generic

text: Chiifu

text: Poly (A+)-mRNA was isolated from total RNA using paramagnetic oligo (dT) beads (PolyAtract mRNA isolation II, Promega) according to the manufacturer's procedures. The cDNAs were cloned into lambda ZAPII (Stratagene) and clones were randomly selected for end sequence verification.

text: Mature green leaf

text: Leaf

text: Brassica rapa subsp. pekinensis

text: EcoRI

text: XhoI

text: mature green leaf cDNA library KHLM

text: pBluescriptII SK(-)


text: live

text: Generic

text: Chiifu

text: etiolated mature leaf

text: Leaf

text: Brassica rapa subsp. pekinensis

text: EcoRI

text: XhoI

text: etiolated mature leaf cDNA library KHLW

text: pBluescriptII SK(-)


text: live

text: Generic

text: Chiifu

text: Poly (A+)-mRNA was isolated from total RNA using paramagnetic oligo (dT) beads (PolyAtract mRNA isolationII, Promega) according to the manufacturer's procedures. The cDNAs were cloned into lambda ZAPII (Stratagene) and random clones selected for end sequence verification.

text: Mixed stage root

text: Root

text: Brassica rapa subsp. pekinensis

text: EcoRI

text: XhoI

text: root cDNA library KHRT

text: pBluescriptII SK(-)


text: ENEA (Italian National Agency for New Technologies,Energy and the Environment) Casaccia Research Center

text: live

text: Generic

text: Mature stigma

text: E. coli SOLR strain

characteristic: lab-host

text: Flower

text: Crocus sativus

text: EcoRI

text: XhoI

text: Hermaphodite

text: Stigma

characteristic: tissue-type

text: Saffron (Crocus sativus) mature stigma lambda Unizap cDNA library

text: Phage

text: Lambda Unizap