text: University of Georgia

characteristic: INSDC center name

text: live

text: Generic

text: The library was prepared from polyA+ RNA from gametophytes extracted from cones on females plants. Double-stranded cDNA was unidirectionally cloned into pBluescript SK- (Stratagene) with the 5' end of the cDNA inserted at the EcoRI site and the 3' polyA+ inserted at Xho1 site. A library-specific adaptor (GCGCTACCG) was used to verify the library identity and then trimmed along with vector and low quality sequence. cDNA Library Statistics: Avg. insert length: 1135; Primary Titer: 1.9E6 pfu total; Amplified Titer: 1.4E10 pfu/ml; Antibiotic: 50 ug/ml Ampicillin. This library was been generated by the Floral Genome Project (FGP; http://fgp.bio.psu.edu) and additional sequencing was done as part of the Ancestral Angiosperm Project (www.AncestralAngiosperm.org). Both projects are funded by NSF's Plant Genome Research Program (DBI-0115684 and DBI-0638595, respectively).

text: premeiotic

text: solr (E. Coli)

text: gametophytes extracted from cones

characteristic: organ

text: Zamia vazquezii

text: Female

text: University of Florida Greenhouse

text: young gametophytes

text: cDNA library derived from female gametophytes

text: Plasmid


status: PUBLIC

characteristic: External Id

text: University of Georgia

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: The library was prepared from polyA+ RNA from gametophytes extracted from cones on male plants. Double-stranded cDNA was unidirectionally cloned into pBluescript SK- (Stratagene) with the 5' end of the cDNA inserted at the EcoRI site and the 3' polyA+ inserted at Xho1 site. A library-specific adaptor (GCGTGACGG) was used to verify the library identity and then trimmed along with vector and low quality sequence. cDNA Library Statistics: Avg. insert length: 1128; Primary Titer: 2.5E6 pfu total; Amplified Titer: 7.5E10 pfu/ml; Antibiotic: 50 ug/ml Ampicillin. This library was been generated by the Floral Genome Project (FGP; http://fgp.bio.psu.edu) and additional sequencing was done as part of the Ancestral Angiosperm Project (www.AncestralAngiosperm.org). Both projects are funded by NSF's Plant Genome Research Program (DBI-0115684 and DBI-0638595, respectively).

characteristic: description

text: premeiotic

tag: attribute

characteristic: dev-stage

text: SOLR (E. Coli)

characteristic: lab-host

text: Developing gametophytes

text: Zamia vazquezii

characteristic: organism

text: Male

characteristic: sex

text: University of Florida Greenhouse

characteristic: strain

text: gametophytes

characteristic: tissue-type

text: Second Zamia male gametophyte library

characteristic: title

text: Plasmid

characteristic: vector


text: Washington University School of Medicine

characteristic: INSDC center name

text: live

text: Generic

text: fat body or intestines

text: Rhodnius prolixus

text: EcorI

text: EcorI

text: Female and Male

text: Rhodnius_prolixus_EST_NADG_Bacteria_Injected

text: plasmid

text: pGEM-T-Easy


text: live

text: Generic

text: fat body or intestines

text: Rhodnius prolixus

text: EcorI

text: EcorI

text: Female and Male

text: Rhodnius_prolixus_EST_NADH_T.cruzi_Injected

text: plasmid

text: pGEM-T-Easy


text: University of Saskatchewan

text: live

text: Generic

text: PI 535272

characteristic: cultivar

text: Subtraction Suppression Hybridization library (Clontech PCR Select) generated by subtracting transcripts of total aerial tissue of 72 hr cold treated (7 deg. C day/5 deg. C night) minus control (23 deg. C day / 18 deg. C night) plants. The inserts are Rsa I restriction fragments of Phaseolus angustissimus cDNA.

text: seedlings (3rd trifoliate leaf stage)

text: Phaseolus angustissimus

text: Total aerial vegetative tissue


characteristic: External Id

text: University of Saskatchewan

text: live

text: Generic

text: PI 535272

text: Subtraction Suppression Hybridization library (Clontech PCR Select) generated by subtracting transcripts of total aerial tissue of control (23 deg. C day / 18 deg. C night)72 hr minus cold treated (7 deg. C day/5 deg. C night) plants. The inserts are Rsa I restriction fragments of Phaseolus angustissimus cDNA.

text: seedlings (3rd trifoliate leaf stage)

text: Phaseolus angustissimus

text: Total aerial vegetative tissue


text: University of Saskatchewan

characteristic: INSDC first public

text: live

text: Generic

text: PI 535272

text: Interspecies Subtraction Suppression Hybridization library (Clontech PCR Select) generated by subtracting transcripts of total aerial tissue of 72 hr cold (7 deg. C day/5 deg. C night)treated plants of Phaseolus angustissimus minus 72 hr cold (7 deg. C day/5 deg. C night)treated plants (3rd trifoliate leaf stage) of Phaseolus vulgaris (cv ICA Pijao). The inserts are Rsa I restriction fragments of P. angustissimus cDNA.

text: seedlings (3rd trifoliate leaf stage)

text: Phaseolus angustissimus

text: Total aerial vegetative tissue


text: University of Saskatchewan

characteristic: INSDC last update

text: live

text: Generic

text: ICA Pijao

text: Interspecies Subtraction Suppression Hybridization library (Clontech PCR Select) generated by subtracting transcripts of total aerial tissue of 72 hr cold (7 deg. C day/5 deg. C night)treated plants of Phaseolus vulgaris (cv ICA Pijao) minus 72 hr cold (7 deg. C day/5 deg. C night)treated plants (3rd trifoliate leaf stage) of Phaseolus angustissimus. The inserts are Rsa I restriction fragments of P. vulgaris cDNA.

text: seedlings (3rd trifoliate leaf stage)

text: Phaseolus vulgaris

text: Total aerial vegetative tissue


text: University of Saskatchewan

text: live

characteristic: INSDC status

text: Generic

text: ICA Pijao

text: Subtraction Suppression Hybridization library (Clontech PCR Select) generated by subtracting transcripts of total aerial tissue of 72 hr cold treated (7 deg. C day/5 deg. C night) minus control (23 deg. C day / 18 deg. C night) plants. The inserts are Rsa I restriction fragments of Phaseolus vulgaris (cv ICA Pijao) cDNA.

text: Seedlings (3rd trifoliate leaf stage)

text: Phaseolus vulgaris

text: Total aerial vegetative tissue


text: University of Saskatchewan

text: live

text: Generic

characteristic: NCBI submission model

text: ICA Pijao

text: Subtraction Suppression Hybridization library (Clontech PCR Select) generated by subtracting transcripts of total aerial tissue of control (23 deg. C day / 18 deg. C night) minus 72 hr cold treated (7 deg. C day/5 deg. C night) plants. The inserts are Rsa I restriction fragments of Phaseolus vulgaris (cv ICA Pijao) cDNA.

text: seedlings (3rd trifoliate leaf stage)

text: Phaseolus vulgaris

text: Total aerial vegetative tissue


text: University of Saskatchewan

text: live

text: Generic

characteristic: NCBI submission package

text: ICA Pijao

text: Interspecies Subtraction Suppression Hybridization library (Clontech PCR Select) generated by subtracting transcripts of total aerial tissue of control (23 deg. C day / 18 deg. C night) plants of Phaseolus vulgaris (cv ICA Pijao) minus control (23 deg. C day / 18 deg. C night) plants (3rd trifoliate leaf stage) of Phaseolus angustissimus. The inserts are Rsa I restriction fragments of P. vulgaris cDNA.

text: seedlings (3rd trifoliate leaf stage)

text: Phaseolus vulgaris

text: Total aerial vegetative tissue


text: University of Saskatchewan

text: live

text: Generic

text: PI 535272

text: Interspecies Subtraction Suppression Hybridization library (Clontech PCR Select) generated by subtracting transcripts of total aerial tissue of control (23 deg. C day / 18 deg. C night) plants of Phaseolus angustissimus minus control (23 deg. C day / 18 deg. C night) plants (3rd trifoliate leaf stage) of Phaseolus vulgaris (cv ICA Pijao). The inserts are Rsa I restriction fragments of P. angustissimus cDNA.

characteristic: description

text: seedlings (3rd trifoliate leaf stage)

text: Phaseolus angustissimus

text: Total aerial vegetative tissue


text: Shizuoka University

text: live

text: Generic

text: Hyuuganatsu

text: anthesis

characteristic: dev-stage

text: Citrus tamurana

text: ovule

text: SSH cDNA library of ovule tissues (Tester: C. tamurana, Driver: C. aurantium)

text: pCR2.1 TOPO


text: Shizuoka University

text: live

text: Generic

text: Natsu-daidai

text: anthesis

text: Citrus natsudaidai

text: ovule

text: SSH cDNA library of ovule tissues (Tester: C. natsudaidai, Driver: C. hassaku)

text: pCR2.1 TOPO


text: Shizuoka University

text: live

text: Generic

text: Hassaku

text: anthesis

text: Citrus hassaku

text: ovule

text: SSH cDNA library of ovule tissues (Tester: C. hassaku, Driver: C. natsudaidai)

text: pCR2.1 TOPO


text: INRA

text: live

text: Generic

text: Library was made using Suppression Subtractive Hybridization method (Diatchenko et al., 1996) from adult leaves collected on a subset of individuals segregating for the unfavourable alleles at a strong QTL for water use efficiency. A mixture of genotypes was used.

text: green leaves on one year old cuttings

text: Quercus robur

text: leaves

text: Quercus robur SSH6

text: pGMT easy


text: INRA

text: live

text: Generic

text: Library was made using Suppression Subtractive Hybridization method (Diatchenko et al., 1996) from adult leaves collected on a subset of individuals segregating for the favourable alleles at a strong QTL for water use efficiency. A mixture of genotypes was used.

text: green leaves on one year old cuttings

text: Quercus robur

text: leaves

text: Quercus robur SSH5

text: pGMT easy


text: Genoscope - Centre National de Sequencage

text: live

text: Generic

text: country: France. Eucalyptus gunnii EST from cold-acclimated leaves (from plantlets in controlled conditions and trees in field trials).

text: Eucalyptus gunnii

text: AFOCEL, 941366

text: mesophyll


text: University of Oklahoma

text: live

text: Generic

text: Sperm cell

characteristic: cell type

text: Svn sperm cells were isolated by using a microinjector. cDNA library were constructed by oligo dT priming using the Clontech SMART cDNA Library Construction kit. The cDNAs were directionally cloned into vector TriplEx2.

text: Isolated from mature pollen of Plumbago zeylanica

text: E.coli strain XL1-Blue

characteristic: lab-host

text: Plumbago zeylanica

text: cDNA library of Plumbago zeylanica Svn sperm cell

text: Plasmid

text: Clontech TriplEx2


text: United Planters' Association of Southern India-Tea Research Foundation, Tea Research Institute

text: live

text: Generic

text: Camellia sinensis

characteristic: organism

text: Sfi I A site

text: Sfi I B site

text: First and second leaves

text: SMP-1 cDNA in pDNR-LIB

text: Plasmid

text: pDNR-LIB (Clonetech)


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected >2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed and sequenced by DOE Joint Genome Institute (Walnut Creek, CA).

text: sexually induced female (mixed stages: 1.5, 10, 24, 48 hr after sexual induction)

text: DH10B T1 resistant

text: Volvox carteri f. nagariensis

text: SalI

text: NotI

text: Female

characteristic: sex

text: Eve

characteristic: strain

text: CBGU Volvox carteri f. nagariensis induced female Eve


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 1.2-2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed and sequenced by DOE Joint Genome Institute (Walnut Creek, CA).

text: DH10B T1 resistant

text: Volvox carteri f. nagariensis

text: SalI

text: NotI

text: Female

text: Eve

text: CBGW Volvox carteri f. nagariensis induced female Eve, 2kb

characteristic: title


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 2-8kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed and sequenced by DOE Joint Genome Institute (Walnut Creek, CA).

text: sexually induced male(mixed stages: 1.5, 10, 24, 48 hr after sexual induction)

text: DH10B T1 resistant

text: Volvox carteri f. nagariensis

text: SalI

text: NotI

text: Male

text: CBGX Volvox carteri f. nagariensis induced male 69-1b, 8kb

characteristic: vector


text: DOE Joint Genome Institute

characteristic: INSDC center name

text: live

text: Generic

text: DH10B T1 resistant

text: Volvox carteri f. nagariensis

text: SalI

text: NotI

text: Male

text: CBGY Volvox carteri f. nagariensis induced male 69-1b, 2kb


text: DOE Joint Genome Institute

text: live

text: Generic

text: DH10B T1 resistant

text: Volvox carteri f. nagariensis

text: SalI

text: NotI

text: Male

text: CBGZ Volvox carteri f. nagariensis induced male 69-1b, 8kb neb notI


text: DOE Joint Genome Institute

text: live

text: Generic

text: DH10B T1 resistant

text: Volvox carteri f. nagariensis

text: SalI

text: NotI

text: Male

text: CBHA Volvox carteri f. nagariensis induced male 69-1b, 2kb neb notI


text: DOE Joint Genome Institute

text: live

text: Generic

text: DH10B T1 resistant

text: Volvox carteri f. nagariensis

text: SalI

text: NotI

text: Female

text: CBHO Volvox carteri f. nagariensis female Eve10 (asexuall, 2-4 cell stage, 32-128 cell stage, inversion)


text: DOE Joint Genome Institute

text: live

text: Generic

text: DH10B T1 resistant

text: Volvox carteri f. nagariensis

text: SalI

text: NotI

text: Female

text: Eve

text: CBOW Volvox carteri f. nagariensis induced female Eve


text: DOE Joint Genome Institute

text: live

text: Generic

text: DH10B T1 resistant

text: Volvox carteri f. nagariensis

text: SalI

text: NotI

text: Female

text: Eve

text: CBOX Volvox carteri f. nagariensis induced female Eve, 2kb


text: Justus-Liebig University of Giessen

text: live

text: Generic

text: subtracted cDNA library

text: adult female unwinged

text: Acyrthosiphon pisum

text: whole animals, Immune challenged

characteristic: tissue-type

text: Acyrthosiphon pisum LSR1 female adult

text: pGEM-T Easy


characteristic: External Id

text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected >2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed and sequencing performed at the DOE Joint Genome Institute (Walnut Creek, CA).

text: 5th instar

text: Pachypsylla venusta

text: SalI

text: NotI

text: both

text: bacteriome

text: CBZU Pachypsylla venusta Pave_0001 H


text: DOE Joint Genome Institute

characteristic: INSDC first public

text: live

text: Generic

text: The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 1.2-2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed and sequencing performed at the DOE Joint Genome Institute (Walnut Creek, CA).

text: 5th instar

text: Pachypsylla venusta

text: SalI

text: NotI

text: both

text: bacteriome

text: CBZW Pachypsylla venusta Pave_0001 M


text: National Institute of Agrobiological Resources

characteristic: INSDC last update

text: live

text: Generic

text: Nipponbare

characteristic: cultivar

text: cDNA prepared from rice callus mRNAs by using oligo(dT) as a primer and ligating to the SalI-NotI site of pBluescript II SK+ phagemid.

text: Oryza sativa Japonica Group

text: SalI

text: NotI

text: Rice callus

text: Phage

text: pBluescript II SK+


text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Nipponbare

text: Prepared from seedling root.

text: seedling

text: Oryza sativa Japonica Group

text: Rice root


text: live

text: Generic

characteristic: NCBI submission package

text: Nipponbare

text: Etiolated shoot (8 days old)

characteristic: description

text: Etiolated shoot (8 days old)

text: Oryza sativa Japonica Group

text: Rice shoot


text: live

text: Generic

text: Nipponbare

text: Green shoot (8 days old)

text: Oryza sativa Japonica Group

text: Rice green shoot


text: Fudan University

text: live

text: Generic

text: Oryza sativa Indica Group

text: Guang-Lu-Ai No.4

text: FDRSC


text: Fudan University

text: live

text: Generic

text: Oryza sativa Indica Group

text: Guang-Lu-Ai No.4

text: root

text: FDRRC


text: live

text: Generic

text: Nipponbare

text: Rice cDNA from panicle at flowering stage

text: flowering stage

characteristic: dev-stage

text: panicle

characteristic: organ

text: Oryza sativa Japonica Group

text: Rice panicle at flowering stage


text: live

text: Generic

text: Nipponbare

text: Rice cDNA from panicle at ripening stage

text: ripening stage

text: panicle

text: Oryza sativa Japonica Group

characteristic: organism

text: Rice panicle at ripening stage


text: Cornell University

text: live

text: Generic

text: A Lambda ZAP II cDNA library was constructed from mRNA extracted from etiolated leaf tissue of the rice cultivar 'IR36' and converted to pBluescript (amp resistant) as described in Causse et al. (1994) Genetics 138:1251-1274. For insert amplification, Use M13 forward and reverse primers. Clones from this library are designated with the prefix 'RZ'.

text: Oryza sativa Indica Group

text: EcoRI

text: cDNA from rice

text: Phage/Phagemid

text: Lambda ZAP II/pBluescript


text: live

text: Generic

text: Nipponbare

text: callus

text: Oryza sativa Japonica Group

text: callus

text: Rice callus cDNA


text: live

text: Generic

text: Nipponbare

text: panicle (between 3cm to 10cm)

text: Oryza sativa Japonica Group

text: panicle

text: Rice panicle (between 3cm to 10cm)


text: live

text: Generic

text: Nipponbare

text: panicle (longer than 10cm)

text: Oryza sativa Japonica Group

text: panicle

text: Rice panicle (longer than 10cm)


text: live

text: Generic

text: Nipponbare

text: etiolated shoot

text: Oryza sativa Japonica Group

text: shoot

text: Rice etiolated shoot


text: live

text: Generic

text: Fujisaka5 (Se-1e, Pi-zt)

text: Oryza sativa Japonica Group

text: leaf

text: Rice from leaf (photoperiod insensitive)


text: live

text: Generic

text: Nipponbare

text: shorter than 3cm

text: panicle

text: Oryza sativa Japonica Group

text: Rice panicle shorter than 3cm


text: live

text: Generic

text: Nipponbare

text: immature leaf including apical meristem (under long day condition)

text: immature

text: leaf

text: Oryza sativa Japonica Group

text: Rice cDNA from immature leaf including apical meristem


text: National Inst. of Agri. Sci. and Tech, RDA

text: live

text: Generic

text: Directional cDNA library inserted into lambda ZAPII vector at 5'end with EcoRI and 3' end with Xho I site.

text: 5 days after pollination

text: E. coli SOLR

characteristic: lab-host

text: Oryza sativa Indica Group

text: EcoRI

text: XhoI

text: Immature Seed

text: Rice Immature Seed Lambda ZAPII cDNA Library

text: Phagemid

text: pBluescript SK(+)


text: Fudan University

text: live

text: Generic

text: Oryza sativa Indica Group

text: Guang-Lu-Ai No.4

characteristic: strain

text: etiolated shoot

text: Rice etiolated shoot cDNA (Y.Jiang)


text: PMBBRC

text: live

text: Generic

text: Millyang 117

text: suspension culture

text: Oryza sativa Indica Group

text: Rice Millyang 117 suspension culture mRNA

characteristic: title


text: University of Tokyo

text: live

text: Generic

text: early embryogenesis

text: Oryza sativa Japonica Group

text: Kamenoo

text: embryo

text: Rice strain Kamenoo early embryogenesis


text: Indian Institute of Science

text: live

text: Generic

text: IR 64

text: early flower, panicle size less than 1 cm

text: Oryza sativa Indica Group

text: UVR lab, Indian Institute of Science; catalog number IR64/early/10-95/kk; ERD series


text: Cornell University

text: live

text: Generic

text: Derived from mRNA extracted from etiolated leaf sheath from the cultivar IR36 (O.sativa).

text: Oryza sativa Indica Group

text: EcoRI Rice Etiolated Leaf cDNA Library

text: pBLUESCRIPT

characteristic: vector


text: Indian Institute of Science

characteristic: INSDC center name

text: live

text: Generic

text: IR 64

text: late flower, panicle size 1-2 cm

text: Oryza sativa Indica Group

text: UVR lab, Indian Institute of Science; catalog number IR64/late/9-95/UVR; LRD series


text: live

text: Generic

text: Nipponbare

text: Oryza sativa Japonica Group

text: Oryza sativa Nipponbare immature leaf including apical meristem (under long day condition)


text: National Institute of Agrobiological Resources

text: live

text: Generic

text: Oryza sativa Japonica Group

text: Nipponbare

text: callus

characteristic: tissue-type

text: benzyladenine treated callus cDNA


characteristic: External Id

text: live

text: Generic

text: Oryza sativa Japonica Group

text: Nipponbare

text: callus

text: gibberellin treated callus cDNA


characteristic: INSDC first public

text: live

text: Generic

text: Oryza sativa Japonica Group

text: Nipponbare

text: callus

text: heat shock callus cDNA


characteristic: INSDC last update

text: live

text: Generic

text: Nipponbare

text: Oryza sativa Japonica Group

text: mature leaf

text: Oryza sativa mature leaf Nipponbare


text: live

text: Generic

text: Nipponbare

characteristic: cultivar

text: Oryza sativa Japonica Group

text: mature leaf

text: Rice mature leaf


text: live

characteristic: INSDC status

text: Generic

text: Nipponbare

text: Oryza sativa Japonica Group

text: young root

text: Rice cDNA from young root


text: live

text: Generic

characteristic: NCBI submission model

text: Nipponbare

text: Oryza sativa Japonica Group

text: Rice cDNA from immature leaf including apical meristem (under short day condition)


text: Universita Cattolica del Sacro Cuore

text: live

text: Generic

characteristic: NCBI submission package

text: seed

text: Oryza sativa Japonica Group

text: Ariete

text: Oryza sativa Ariete seed


text: live

text: Generic

text: Nipponbare

text: Oryza sativa Japonica Group

text: Rice immature leaf including apical meristem (under long day condition)


text: National Taiwan University

text: live

text: Generic

text: Taichung Native 1

text: seedling

text: Oryza sativa Indica Group

text: root

text: Oryza sativa subsp. indica root seedling

text: pGEM-T Easy


text: National Taiwan University

text: live

text: Generic

text: Tainung 67

text: seedling

text: Oryza sativa Japonica Group

text: root

text: Oryza sativa subsp. japonica root seedling

text: pGEM-T Easy


text: University of Illinois

text: live

text: Generic

text: 1 d 150mM NaCl

characteristic: description

text: 1 week

text: Oryza sativa Indica Group

text: Pokkali

text: roots

text: OD


text: University of Illinois

text: live

text: Generic

text: 1 week 150mM NaCl

text: 2 weeks

characteristic: dev-stage

text: Oryza sativa Indica Group

text: Pokkali

text: entire plant

text: OF


text: University of Illinois

text: live

text: Generic

text: 2-3 d 150mM NaCl

text: 1 week

text: Oryza sativa Indica Group

text: Pokkali

text: roots

text: OE


text: University of Illinois

text: live

text: Generic

text: no stress

text: 1 week

text: Oryza sativa Indica Group

text: Pokkali

text: roots

text: OC


text: University of Illinois

text: live

text: Generic

text: 19 h 200mM NaCl

text: 3-4 weeks

text: Oryza sativa Japonica Group

text: Nipponbare

text: roots

text: OA


text: Toyama Agricultural Research Center

text: live

text: Generic

text: callus

characteristic: cell type

text: Nipponbare

text: Oryza sativa Japonica Group

text: rice haploid callus


text: Toyama Agricultural Research Center

text: live

text: Generic

text: callus

text: Nipponbare

text: Oryza sativa Japonica Group

characteristic: organism

text: rice diploid callus


text: live

text: Generic

text: Directional cDNA library inserted into lambda ZAPII vector at 5'end with EcoRI and 3' end with Xho I site

text: 5 days after pollination

text: E. coli SOLR

characteristic: lab-host

text: Oryza sativa Indica Group

text: EcoRI

text: XhoI

text: Rice Seedling Lambda ZAPII cDNA Library

text: pBluescript SK(+)


text: plantechno srl

text: live

text: Generic

text: Ariete

text: Seed

text: Oryza sativa Japonica Group

text: Oryza sativa Ariete Seed


text: Hauzhong Agricultural University

text: live

text: Generic

text: Minghui 63

text: 14 different tissues including callus, seedlings (etiolated, three-leaf stage, and five-leaf stage), culms (tillering stage and flowering stage), roots, flag leaves, panicles (flowering stage and two different stages of grain-filling), flowers, leaves after Xanthomonas oryzae pv oryzae (strain PXO61) inoculation, and leaves after Pyricularia grisea (isolate 1814) inoculation

text: Oryza sativa Indica Group

text: Not I

text: Sal I

text: 14 different tissues

text: Normalized rice cDNA library

text: plasmid

text: pSPORT 1


text: University of Nevada

text: live

text: Generic

text: Pokkali

text: 7 day-old (treated with 150 mM NaCl for 1, 2 and 7 days).

text: Escherichia coli

text: Oryza sativa Indica Group

text: EcoRI

text: XhoI

text: leaf

text: OG

text: Plasmid

text: UNI-ZAP XR, pBluescriptSK- (Stratagene)


text: Sichuan University

text: live

text: Generic

text: Sperm cell

text: Tester cDNA was prepared from isolated rice sperm cells by PCR with the help of SMART PCR cDNA Synthesis Kit(Clontech) and driver cDNA was prepared from two-cell stage pollen of rice. Subtraction was carried out with the help of CLONTECH PCR-Select cDNA Subtraction kit. Subtracted cDNAs were inserted into pUCm-T plasmid.

text: Isolated from mature pollen of rice

text: E.coli JM109

text: Oryza sativa Indica Group

text: Subtracted rice sperm cells cDNA Library

text: plasmid

text: pUCm-T


text: University of Arkansas

text: live

text: Generic

text: Drew (a major cultivated variety in Arkansas)

text: Rice seedling leaves were pretreated with 0.3 mM cyclohexmide (CHX) half an hour before treatment of 0.2 mM jasmonic acid (JA) or 0.4 mM benzothiadiazole (BTH). Both JA- and BTH-induced mRNAs were equally pooled for subtracted cDNA library construction

text: 16-day-old rice seedling treated by JA/BTH

text: Oryza sativa Japonica Group

text: Seedling leaves

text: Subtracted cDNA library of JA/BTH-treated rice leaf

text: pGEM-T easy


text: University of Arkansas

text: live

text: Generic

text: Rice seedling leaves were infected with blast fungus (Magnaporthe grisea, 750,000 spores /ml). Leaves from 1 to 4 days after infection were collected for cDNA library construction

text: 16-day-old rice seedling infected by Magnaporthe grisea

text: Oryza sativa Japonica Group

text: Seedling leaves

text: Magnaporthe grisea infected 16-day-old rice seedling cDNA library

text: lambda ZAP Express


text: National Institute of Agrobiological Resources

text: live

text: Generic

text: mangetumochi

text: Oryza sativa Japonica Group

text: seed

text: Rice cDNA from seed


text: live

text: Generic

text: Oryza sativa Japonica Group

text: leaf

text: Rice cDNA from leaf


text: live

text: Generic

text: Nipponbare

text: Oryza sativa Japonica Group

text: callus

text: Rice callus (2001)


text: University of Hyderabad

text: live

text: Generic

text: Nagina 22 (indica sub sp)

text: ESTs from normalized leaf cDNA Library from drought stressed seedlings

text: 35 day-old seedlings

text: Leaf

characteristic: organ

text: Oryza sativa Indica Group

text: Entire leaf tissue

text: Drought stress (leaf)

text: Phagemid


text: Academia Sinica

text: live

text: Generic

text: Tainoung 67

text: cDNA prepared from rice young panicle mRNA by using oligo(dT) as the primer for ligation into the SfiIA-SfiIB sites of phagemid pTripleEx2.

text: 5mm long panicle

text: Oryza sativa Japonica Group

text: japonica

characteristic: strain

text: panicle

text: Rice young panicles

characteristic: title

text: phagemid


text: South China Institute of Botany, Acadamia Sinica of China

characteristic: INSDC center name

text: live

text: Generic

text: Stem

text: Oryza sativa Japonica Group

text: Zhong A

text: Oryza sativa Zhong A strain stem

text: pMD18-T vector

characteristic: vector


characteristic: External Id

text: University of Delhi South Campus

text: live

text: Generic

text: Pusa Basmati-1

text: Oryza sativa Indica Group

text: Oryza sativa cv. Pusa Basmati-1


text: Institute of Genetics, Chinese Academy of Sciences

characteristic: INSDC first public

text: live

text: Generic

text: Lansheng

text: two weeks old seedling

text: all plant

text: Oryza sativa Japonica Group

text: Rice Uni Zap Library


text: College of Life Sciences, Zhejiang University

characteristic: INSDC last update

text: live

text: Generic

text: Azucena

text: 10-day seedling

text: Oryza sativa Japonica Group

text: root

characteristic: tissue-type

text: Upland-induced root


text: National Crop Experiment Station Seodundong

text: live

characteristic: INSDC status

text: Generic

text: variety=Daegoldo; isolated under low temperature conditions

text: Oryza sativa Japonica Group

text: Oryza sativa (japonica cultivar-group) var. Daegoldo


text: Zhongshan University

text: live

text: Generic

characteristic: NCBI submission model

text: Bianyiyuexiangzhan

characteristic: cultivar

text: seedling five leaf stage

text: Oryza sativa Indica Group

text: leaf

text: Oryza sativa (indica cultivar-group) cv. Bianyiyuexiangzhan leaf


text: Zhongshan University

text: live

text: Generic

characteristic: NCBI submission package

text: Yuexiangzhan

text: seedling five leaf stage

text: Oryza sativa Indica Group

text: leaf

text: Oryza sativa (indica cultivar-group) cv. Yuexiangzhan leaf


text: Zhongshan University

text: live

text: Generic

text: Bianyiheigu

text: seedling five leaf stage

characteristic: dev-stage

text: Oryza sativa Japonica Group

text: leaf

text: Oryza sativa (japonica cultivar-group) cv. Bianyiheigu leaf


text: Zhongshan University

text: live

text: Generic

text: lijiangxintuanheigu

text: seedling five leaf stage

text: Oryza sativa Japonica Group

text: leaf

text: Oryza sativa (japonica cultivar-group) cv. lijiangxintuanheigu leaf


text: University of Arkansas

text: live

text: Generic

text: The cDNAs were derived from reverse transcription of mRNA samples from seeds at different stages of germination and seedlings at early phase of growth under chilling stress (13oC/10oC). The mRNA pool was used as template for double stranded cDNA synthesis using the Stratagene Uni-Zap XR cDNA synthesis and library kit. A total of 150,000 phages were excised from the primary library as pBluescript phagemid clones. Enrichment of the primary excised library with chilling-induced transcripts was performed by hybridizing the primary excised library colony lifts with the PCR-select subtraction product, with cold germinated cDNA as tester and control temperature-germinated cDNA as driver.

characteristic: description

text: embryo at different stages of germination and shoots of germinated seeds under chilling stress (13oC/10oC)

text: SolR

text: seedlings

text: Oryza sativa Japonica Group

text: EcoRI

text: XhoI

text: coleotile, radicle, prophyll, immature leaf

text: Rice cold stress germination cDNA library

text: plasmid

text: Lambda Uni-Zap XR excised as pBluescript


text: Mie University

text: live

text: Generic

text: Koshihikari

text: tri-nucleate pollen stage

text: Oryza sativa Japonica Group

characteristic: organism

text: pistil

text: Oryza sativa Koshihikari pistil


text: Institute of Genetics, Fuand University

text: live

text: Generic

text: express sequence tags by root-driving leaf SSH in rice

text: Oryza sativa Indica Group

text: leaf

text: root-driving leaf SSH in rice

text: pGEM-T


text: University of Illinois

text: live

text: Generic

text: Water stress was applied by not watering for 4 consecutive days. Panicles were collected from control (well watered) and stressed plants at 2 days before heading, heading, 50% flowering and 4 days after 50% flowering.

text: Flowering

text: Oryza sativa Indica Group

text: Panicles

text: IRRI clones

text: pBluescript II SK+


text: International Rice Research Institute

text: live

text: Generic

text: Water stress was applied by not watering for 4 consecutive days. Panicles were collected from control (well watered) and stressed plants at 2 days before heading, at heading, 50% flowering and 4 days after 50% flowering.

text: Flowering

text: Oryza sativa Indica Group

text: Panicles

text: IRRI Drought Stress Panicle Library

text: pBluescript II SK+


text: Plant Genome Center Co.,Ltd

text: live

text: Generic

text: Nipponbare

text: Oryza sativa Japonica Group

text: phloem

text: lambda TriplEx2 rice phloem sap cDNA


text: Chinese Academic of Sciences

text: live

text: Generic

text: Oryza sativa Indica Group

text: Rice hybrid peiai64s 9311


text: The University of Tokyo

text: live

text: Generic

text: 10 days after anthesis

text: Oryza sativa Japonica Group

text: endosperm

text: Oryza sativa endosperm 10 days after anthesis


text: Rice Institute, Nanjing Agricultual University

text: live

text: Generic

text: jiu caiqing

text: country=China

text: seedling

text: Oryza sativa Japonica Group

text: Oryza sativa (japonica cultivar-group) jiu caiqing seedling


text: The University of Arizona

text: live

text: Generic

text: Lesion Mimic SPL 11

text: 3 week

characteristic: lab-host

text: Oryza sativa Indica Group

text: EcoRI

text: XhoI

text: Leaf

text: OSIIEa

text: Plasmid

text: pBluescript II KS +


text: The University of Arizona

text: live

text: Generic

text: 24 hrs after innoculation with Rice Blast (PO6-6-3)

text: 3 week

text: Oryza sativa Indica Group

text: EcoRI

text: XhoI

text: Leaf

text: OSIIEb

text: Plasmid

text: pBluescript II KS +


text: The University of Arizona

text: live

text: Generic

text: Nipponbare

text: 6 hrs after innoculation with Rice Blast (Che 86061)

text: 3 week

text: Oryza sativa Japonica Group

text: EcoRI

text: XhoI

text: Leaf

text: OSJNEa

text: Plasmid

text: pBluescript II KS +


text: The University of Arizona

text: live

text: Generic

text: Nipponbare

text: 24 hrs after innoculation with Rice Blast (Che 86061)

text: 3 week

text: Oryza sativa Japonica Group

text: EcoRI

text: XhoI

text: Leaf

text: OSJNEb

text: Plasmid

text: pBluescript II KS +


text: The University of Arizona

text: live

text: Generic

text: Nipponbare

text: 6 hrs after innoculation with Rice Blast (C9240-1)

text: 3 week

text: Oryza sativa Japonica Group

text: EcoRI

text: XhoI

text: Leaf

text: OSJNEc

text: Plasmid

text: pBluescript II KS +


text: The University of Arizona

text: live

text: Generic

text: Nipponbare

text: 24 hrs after innoculation with Rice Blast (C9240-1)

text: 3 week

text: Oryza sativa Japonica Group

text: EcoRI

text: XhoI

text: Leaf

text: OSJNEd

text: Plasmid

text: pBluescript II KS +


text: The University of Arizona

text: live

text: Generic

text: Nipponbare

text: 24 hrs after innoculation with Rice Blast (70-15)

text: 3 week

text: Oryza sativa Japonica Group

text: EcoRI

text: XhoI

text: Leaf

text: OSJNEe

text: Plasmid

text: pBluescript II KS +


text: The University of Arizona

text: live

text: Generic

text: Nipponbare

text: Uninfected Control

text: 3 week

text: Oryza sativa Japonica Group

text: EcoRI

text: XhoI

text: Leaf

text: OSJNEf

text: Plasmid

text: pBluescript II KS +


text: Chinese Academy of Sciences

text: live

text: Generic

text: Oryza sativa Indica Group

text: Oryza sativa (indica cultivar-group) library China


text: Hauzhong Agricultural University

text: live

text: Generic

text: The library is constructed from the subtraction of inoculated-IRBB13 by non-inoculated-IRBB13 and the subtraction of inoculated-IRBB13 by inoculated-IR24. IR24 is the susceptible near-isogenic parental line of IRBB13. The inoculation pathogen is Xanthomonas oryzae pv. oryzae (Philippine strain PXO99)

text: booting stage

text: E. coli DH10B

text: Oryza sativa Indica Group

text: RsaI

text: leaf

text: Rice pathogen-induced subtraction cDNA library

text: plasmid

text: pGEM-T


text: The Ohio State University

text: live

text: Generic

text: 75-1-127 and IR31917

text: Rice 21-day-old plants were inoculated with blast isolate PO6-6 (conidia concentration: 100,000 spores/ml). Leaves from 12 and 24 hours after inoculation were collected and mixed equally for SSH cDNA library construction

text: 21-day-old rice seedling infected by Magnaporthe grisea

text: Oryza sativa Indica Group

text: EcoRI

text: EcoRI

text: Seedling leaves

text: Magnaporthe grisea infected SSH cDNA library

text: plasmid

text: pGEM-T easy vector


text: Graduate school of Hiroshima University, Faculty of Science

text: live

text: Generic

text: germ cell generating stage

text: Oryza sativa Japonica Group

text: panicle

text: pNS rice panicle cDNA, germ cell generating stage


text: live

text: Generic

text: flowering stage

text: Oryza sativa Japonica Group

text: panicle

text: pNS rice panicle cDNA, flowering stage


text: live

text: Generic

text: 4 days after regeneration treatment

text: Oryza sativa Japonica Group

text: callus

text: pNS rice callus cDNA


text: Japan International Research Center for Agricultural Sciences

text: live

text: Generic

text: Nipponbare

text: seedling

text: Oryza sativa Japonica Group

text: whole plant

text: Oryza sativa (japonica cultivar-group) whole plant seedling


text: IRD

text: live

text: Generic

text: azucena

text: Sample name: AZ-BI; Plant growth place: greenhouse; Soil conditions: standard; Sowing date: 28/02/2000; Harvesting date: 18/03/2000; Stress date: 15/03/2000; Cultivar: azucena; Description: Az-wounded leaves cDNA library; experimental condition: wound;

text: 3 weeks-old plants

text: SOLR strain (Stratagene)

text: Oryza sativa Japonica Group

text: Eco RI

text: Xho I

text: leaves

text: AZ-BI

text: pBluescript SK (+/-)


text: IRD

text: live

text: Generic

text: azucena

text: Sample name: AZ-VI; Plant growth place: greenhouse; Soil conditions: standard; Sowing date: 28/02/2000; Harvesting date: 18/03/2000; Stress date: 15/03/2000; Cultivar: azucena; Description: Az-RYMV inoculated leaves cDNA library; experimental condition: rymv inoculation;

text: 3 weeks-old plants

text: SOLR strain (Stratagene)

text: Oryza sativa Japonica Group

text: Eco RI

text: Xho I

text: leaves

text: AZ-VI

text: pBluescript SK (+/-)


text: IRD

text: live

text: Generic

text: Sample name: IR64-BI; Plant growth place: greenhouse; Soil conditions: standard; Sowing date: 28/02/2000; Harvesting date: 17/03/2000; Stress date: 15/03/2000; Cultivar: ir64; Description: IR64-woundeed leaves cDNA library; experimental condition: wound;

text: 3 weeks-old plants

text: SOLR strain (Stratagene)

text: Oryza sativa Indica Group

text: Eco RI

text: Xho I

text: leaves

text: pBluescript SK (+/-)


text: IRD

text: live

text: Generic

text: Sample name: IR64-VI; Plant growth place: greenhouse; Soil conditions: standard; Sowing date: 28/02/2000; Harvesting date: 17/03/2000; Stress date: 15/03/2000; Cultivar: ir64; Description: IR64-RYMV inoculated leaves cDNA library; experimental condition: rymv inoculation;

text: 3 weeks-old plants

text: SOLR strain (Stratagene)

text: Oryza sativa Indica Group

text: Eco RI

text: Xho I

text: leaves

text: pBluescript SK (+/-)


text: Shanghai Institute of Plant Physiology and Ecology

text: live

text: Generic

text: Oryza sativa Japonica Group

text: Oryza sativa (japonica cultivar-group) library (Chu ZQ)


text: School of Life Sciences and Biotechnology, Korea University

text: live

text: Generic

text: Using the gene specific primer based on the nucleotide of Copia type retrotransposon (OGRE-1 from O. grandiglumis Acc. No. 101154), a fragment (780 bp size) of the reverse transcriptase was amplified and cloned.

text: Vegetative stage

text: Oryza sativa Indica Group

text: leaf

text: Oryza sativa cv. Milyang23 PCR and T/A cloning

text: Phagemid


text: Research Institute of Forestry, Chinese Academy of Forestry

text: live

text: Generic

text: seedlings

text: E.coli

text: Oryza sativa Indica Group

text: Han Dao 297(upland rice)

text: Rice seedlings under drought stress at induced early stage

text: pGEM-T easy


text: School of Life Science, Sun Yat-sen University

text: live

text: Generic

text: Rice cDNA from panicle at pollen meiosis stage

text: pollen meiosis stage

text: panicle shorter than 3cm

characteristic: organ

text: Oryza sativa Indica Group

text: rice panicle shorter than 3cm

text: Plasmid

text: pbluescript sk+


text: Key Lab of Ministry of Education for Plant Developmental Biology, College of Life Science, Wuhan University

text: live

text: Generic

text: cDNA clones generated from rice cDNA-AFLP products.

text: Oryza sativa Indica Group

text: rice cDNA-AFLP products


text: University of Illinois

text: live

text: Generic

text: Nipponbare

text: 19 h 200mM NaCl

text: 3-4 weeks

text: Oryza sativa Japonica Group

text: Leaf

text: OB

text: pBluescript II SK(+) XR Predigested vector


text: Nara Institute of Science and Technology

text: live

text: Generic

text: suspension culture

characteristic: cell type

text: Oc

characteristic: cell-line

text: specimen_voucher: Oc-MA-2002 (NAIST, Japan); country: Japan; induced by incompatible N1141 strain of Acidovorax avenae

text: Oryza sativa Indica Group

text: ZAPII rice Oc cDNA


characteristic: INSDC center name

text: live

text: Generic

text: Oc

text: specimen_voucher=Oc-MA-2002 (NAIST, Japan); tissue_lib=Oc-N1; country=Japan; incompatible strain (Acidovorax avenae)-inoculated cultured rice cells

text: Oryza sativa Indica Group

text: ZAPII cultured rice cells cDNA

characteristic: title


characteristic: External Id

text: IBP-CNRS, University of Paris XI

text: live

text: Generic

text: Nipponbare

text: RT-PCR were performed on shoot apex RNA. Resulting amplification product was cloned in a pGEM-T easy vector (Promega) and SP6/T7 sequenced.

text: 3-5 leaves plantlets

text: shoot apex

text: Oryza sativa Japonica Group

text: hermaphrodite

characteristic: sex

text: shoot apex

text: PCR amplification from tel cDNA (rice)

text: pGEM-T easy vector (Promega)

characteristic: vector


text: National Institute of Agricultural Biotechnology, Rural Development Administration

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Ilpumbyeo

text: Cold treatment by placing at 4oC for 16 hour after growth on MS medium for one week

text: one week old

text: Oryza sativa Japonica Group

text: Leaf

characteristic: tissue-type

text: Cold stressed rice cDNA library

text: pBluescript SK+


text: Niigata University

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Nipponbare

characteristic: cultivar

text: 14-day-old etiolated seedling

text: Oryza sativa Japonica Group

text: leaf

text: 3 hr light cDNA library


text: Genomics and Genetics Institute, GreenGene Biotech Inc.; Division of Bioscience and Bioinformatics, MyongJi University

text: live

text: Generic

characteristic: NCBI submission package

text: Nackdong

text: cDNA was inserted into lamda Uni-ZAP XR vector at 5' end with EcoRI and 3' end with XhoI site.

characteristic: description

text: 14 days after germination

characteristic: dev-stage

text: E.coli SOLR

text: Oryza sativa Japonica Group

text: EcoRI

text: XhoI

text: root

text: Rice root lambda phage cDNA library (root)

text: phagemid

text: pBluescript SK(+)


text: live

text: Generic

text: Nackdong

text: cDNA was inserted into HybriZAP-2.1 vector at 5' end with EcoRI and 3' end with XhoI site.

text: Before heading

text: E.coli XLOLR

characteristic: lab-host

text: Oryza sativa Japonica Group

characteristic: organism

text: EcoRI

text: XhoI

text: spikelet

text: Rice before heading yeast two hybrid lambda phage cDNA library (YBH)

text: plasmid


text: live

text: Generic

text: Nackdong

text: etiolated rice

text: E.coli XLOLR

text: Oryza sativa Japonica Group

text: EcoRI

text: XhoI

text: leaf

text: Etiolated rice leaf yeast two hybrid lambda phage cDNA library (YDARK)

text: plasmid


text: live

text: Generic

text: Nackdong

text: mRNA was extracted from TPSP overexpression line. cDNA was inserted into HybriZAP-2.1 vector at 5' end with EcoRI and 3' end with XhoI site.

text: 14 days after germination

text: E.coli XLOLR

text: Oryza sativa Japonica Group

text: EcoRI

text: XhoI

text: leaf

text: TPSP-overexpression transgenic rice yeast two hybrid lambda phage cDNA library (YTPSP)

text: plasmid


text: live

text: Generic

text: Nackdong

text: Leaf was incubated at 4 C(360uM/m-2sec-1) for 2hrs. cDNA was inserted into lamda Uni-ZAP XR vector at 5' end with EcoRI and 3' end with XhoI site.

text: 14 days after gernimation

text: E.coli SOLR

text: Oryza sativa Japonica Group

text: EcoRI

text: XhoI

text: leaf

text: Salt treated rice leaf lambda phage cDNA library (14Salt)

text: phagemid

text: pBluescript SK(+)


text: live

text: Generic

text: Nackdong

text: Leaf was dried for 2hrs. cDNA was inserted into lamda Uni-ZAP XR vector at 5' end with EcoRI and 3' end with XhoI site.

text: 14 days after gernimation

text: E.coli SOLR

text: Oryza sativa Japonica Group

text: EcoRI

text: XhoI

text: leaf

text: Cold treated rice leaf lambda phage cDNA library (COLD)

text: phagemid

text: pBluescript SK(+)


text: live

text: Generic

text: Nackdong

text: E.coli SOLR

text: Oryza sativa Japonica Group

text: EcoRI

text: XhoI

text: flower

text: Rice flower lambda phage cDNA library (FLO)

text: phagemid

text: pBluescript SK(+)


text: University of Arkansas

text: live

text: Generic

text: This sequence was obtained by cDNA-AFLP where cDNAs were synthesized from reverse transcription of mRNA isolated from seeds at different stages of germination and seedlings at early phase of growth under chilling stress (13C/10C). cDNA was digested with EcoRI and MseI restriction endonucleases simultaneously. Subsequently, the cDNA fragments were ligated to EcoRI and MseI adapters to generate template DNA for amplification. These common adapter sequences flanking variable cDNA sequences serve as the primer binding sites on these restriction fragments. Small DNA fragments were generated, then amplified by PCR using different primer combinations including two and three selective bases in the presence of 33P for radioactive labeling of the amplified fragments. The PCR products of the cold-treated and control cDNAs were run on a denaturing polyacrylamide gel. The up-regulated bands were isolated from the gel, cloned in pCR2.1 vector (Invitrogen) and sequenced in one direction using the T3 primer.

text: Coleoptile and radicle stages

text: leaf

characteristic: organ

text: Oryza sativa Japonica Group

text: EcoRI

text: MseI

text: Japonica

characteristic: strain

text: leaf

text: Rice cold stress germination cDNA-AFLP

text: plasmid


text: Hauzhong Agricultural University

characteristic: INSDC center name

text: live

text: Generic

text: Minghui 63

text: The library is constructed based on the strategy of satuation hybridization with genomic DNA using rice cultivar Minghui 63. This library consists of cDNA from 15 directionally cloned cDNA libraries constructed with different tissues from 9 developmental stages.

text: whole-life-cycle

text: E. coli DH10B

text: Oryza sativa Indica Group

text: SalI

text: NotI

text: indica

text: whole plant

text: A normalized whole-life-cycle cDNA library of rice

characteristic: title

text: plasmid


characteristic: External Id

text: Hitachi, Ltd.

text: live

text: Generic

text: Dee-Geo-Woo-Gen (DGWG)

text: 250mM NaCl for 5 h

text: 2 weeks

text: Oryza sativa Indica Group

text: seedling

text: Oryza sativa (indica cultivar-group) seedling 2 weeks


text: Indian Agricultural Research Institute

text: live

text: Generic

text: Tetep

text: Rice seedlings innoculated with Magnaporthe grisea

text: Oryza sativa Indica Group

text: Sfi1 site A

text: Sfi1A site B

text: Leaf

text: Rice SMART cDNA library

text: Phage

text: Lambda TriplEx2

characteristic: vector


text: University of Delhi, South Campus

characteristic: INSDC first public

text: live

text: Generic

text: Oryza sativa Indica Group

text: Oryza sativa (indica cultivar-group) library (Agarwal S)


text: University of Delhi South Campus

characteristic: INSDC last update

text: live

text: Generic

text: Oryza sativa Indica Group

text: Oryza sativa (indica cultivar-group) salt stress associated EST


text: University of Delhi South Campus

text: live

text: Generic

text: Oryza sativa Indica Group

text: Oryza sativa (indica cultivar-group) library (Singh A)


text: Huazhong Agricultural University

text: live

text: Generic

text: Minghui 63

text: The 2 libraries are constructed based on the strategy of cDNA subtractive hybridization using rice cultivar Minghui 63. These two subtractive libraries consists of ESTs differentially expressed after Chilo suppressalis attack in the rice sheath at tillering stage.

text: Tillering stage

text: E. coli DH10B

text: Oryza sativa Indica Group

text: sheath

characteristic: tissue-type

text: Forward and reverse subtractive library of rice sheath after Chilo suppressalis attack

text: plasmid

text: pGEM-T


text: University of Delhi, South Campus

text: live

characteristic: INSDC status

text: Generic

text: Oryza sativa Indica Group

text: Oryza sativa (indica cultivar-group) cv. FR-13A


text: University of Wisconsin

text: live

text: Generic

characteristic: NCBI submission model

text: Nipponbare

text: The library contains RT-PCR products derived from CRR (Centromeric Retrotransposon of Rice). Total RNA was reversely transcribed into cDNA using random priming method. The cDNA was used as a template for PCR amplification using CRR-specific primers (CRR1/2_1F: 5' GTA CTC CTC YGC TAG TGA TTT MGA 3' and CRR1/2_2R: 5' ACT TGA CGC TGA ATC TCC TT 3').

text: four weeks old plant

text: leaf

text: Oryza sativa Japonica Group


text: University of Wisconsin

text: live

text: Generic

text: Nipponbare

characteristic: cultivar

text: The library contains RT-PCR products derived from CRR (Centromeric Retrotransposon of Rice). Total RNA was reversely transcribed into cDNA using random priming method. The cDNA was used as a template for PCR amplification using CRR-specific primers (CRR1/2_2F: 5' AAG GAG ATT CAG CGT CAA GT 3' and CRR1/2_3R: 5' CAA TCC CAC TAG CAT CAC ATT 3').

text: four weeks old plant

text: leaf

text: Oryza sativa Japonica Group


text: University of Wisconsin

text: live

text: Generic

characteristic: NCBI submission package

text: Nipponbare

text: The library contains RT-PCR products derived from CRR (Centromeric Retrotransposon of Rice). Total RNA was reversely transcribed into cDNA using random priming method. The cDNA was used as a template for PCR amplification using CRR-specific primers (CRR1/2_3F: 5' AAT GTG ATG CTA GTG GGA TTG 3' and CRR1/2_4R: 5' TAG CCC ACA ARG TTC TCC A 3').

text: four weeks old plant

text: leaf

text: Oryza sativa Japonica Group


text: University of Wisconsin

text: live

text: Generic

text: Nipponbare

text: The library contains RT-PCR products derived from CRR (Centromeric Retrotransposon of Rice). Total RNA was reversely transcribed into cDNA using random priming method. The cDNA was used as a template for PCR amplification using CRR-specific primers (CRR1/2_4F: 5' TGG AGA ACY TTG TGG GCT A 3' and CRR_ppt1R: 5' NCA TCA TCC TCC CCT TCT T 3').

characteristic: description

text: four weeks old plant

text: leaf

text: Oryza sativa Japonica Group


text: University of Wisconsin

text: live

text: Generic

text: Nipponbare

text: four weeks old plant

characteristic: dev-stage

text: root

text: Oryza sativa Japonica Group


text: University of Wisconsin

text: live

text: Generic

text: Nipponbare

text: four weeks old plant

text: root

text: Oryza sativa Japonica Group


text: University of Wisconsin

text: live

text: Generic

text: Nipponbare

text: The library contains RT-PCR products derived from CRR (Centromeric Retrotransposon of Rice). Total RNA was reversely transcribed into cDNA using random priming method. The cDNA was used as a template for PCR amplification using CRR-specific primers (CRR1_2F: 5' CTC CTC TCA TCG AAA GAT CG 3' and CRR1_3R: 5' GGC TGC AAG TAA TAT CAA GGT AC 3').

text: four weeks old plant

text: leaf

text: Oryza sativa Japonica Group


text: University of Wisconsin

text: live

text: Generic

text: Nipponbare

text: The library contains RT-PCR products derived from CRR (Centromeric Retrotransposon of Rice). Total RNA was reversely transcribed into cDNA using random priming method. The cDNA was used as a template for PCR amplification using CRR-specific primers (CRR1_3F: 5' GTA CCT TGA TAT TAC TTG CAG CC 3' and CRR1_4R: 5' AAT GTC CTG AAT TTC TCG GTT 3').

text: four weeks old plant

text: leaf

text: Oryza sativa Japonica Group


text: University of Wisconsin

text: live

text: Generic

text: Nipponbare

text: The library contains RT-PCR products derived from CRR (Centromeric Retrotransposon of Rice). Total RNA was reversely transcribed into cDNA using random priming method. The cDNA was used as a template for PCR amplification using CRR-specific primers (CRR1_4F: 5' AAC CGA GAA ATT CAG GAC ATT 3' and CRR1_5R: 5' TGT ACT CTT ACC AAT CGT GTT ACC 3').

text: four weeks old plant

text: leaf

text: Oryza sativa Japonica Group


text: University of Wisconsin

text: live

text: Generic

text: Nipponbare

text: The library contains RT-PCR products derived from CRR (Centromeric Retrotransposon of Rice). Total RNA was reversely transcribed into cDNA using random priming method. The cDNA was used as a template for PCR amplification using CRR-specific primers (CRR2_2F: 5' CCT TAC CTC TCA TCG AAA GAT C 3' and CRR2_3R: 5' AAA GTT CTA TGC GAA TAG GTC C 3').

text: four weeks old plant

text: leaf

text: Oryza sativa Japonica Group


text: University of Wisconsin

text: live

text: Generic

text: Nipponbare

text: The library contains RT-PCR products derived from CRR (Centromeric Retrotransposon of Rice). Total RNA was reversely transcribed into cDNA using random priming method. The cDNA was used as a template for PCR amplification using CRR-specific primers CRR2_3F: 5' GGA CCT ATT CGC ATA GAA CTT T 3' and CRR2_4R: 5' CAA ATC TGT ATC AAG TCC TTC TGT 3').

text: four weeks old plant

text: leaf

text: Oryza sativa Japonica Group


text: University of Wisconsin

text: live

text: Generic

text: Nipponbare

text: The library contains RT-PCR products derived from CRR (Centromeric Retrotransposon of Rice). Total RNA was reversely transcribed into cDNA using random priming method. The cDNA was used as a template for PCR amplification using CRR-specific primers (NOACRR1_2F: 5' AGW GGA ACT CAG GGT TCA TC 3' and NOACRR1_3R: 5' GTA CCG TGC TGG TCC TAG G 3').

text: four weeks old plant

text: leaf

text: Oryza sativa Japonica Group


text: University of Wisconsin

text: live

text: Generic

text: Nipponbare

text: The library contains RT-PCR products derived from CRR (Centromeric Retrotransposon of Rice). Total RNA was reversely transcribed into cDNA using random priming method. The cDNA was used as a template for PCR amplification using CRR-specific primers (NOACRR1_4F: 5' AYR TTR ATT GGG ARC TAG CRG 3' and NOACRR1_5R: 5' CAY AAC TCT TCT TTA TCA CAA GGA A 3').

text: four weeks old plant

text: leaf

text: Oryza sativa Japonica Group


text: University of Wisconsin

text: live

text: Generic

text: Nipponbare

text: The library contains RT-PCR products derived from CRR (Centromeric Retrotransposon of Rice). Total RNA was reversely transcribed into cDNA using random priming method. The cDNA was used as a template for PCR amplification using CRR-specific primers (NOACRR1_5F: 5' TTC CTT GTG ATA AAG AAG AGT TRT G 3' and CRR_ppt1R: 5' NCA TCA TCC TCC CCT TCT T 3').

text: four weeks old plant

text: leaf

text: Oryza sativa Japonica Group


text: University of Wisconsin

text: live

text: Generic

text: Nipponbare

text: The library contains 3'RACE products derived from CRR (Centromeric Retrotransposon of Rice). Total RNA was reversely transcribed into cDNA using 3'RACE_oligoT primer (5' GGC CAC GCG TCG ACT AGT ACT TTT TTT TTT TTT TTT TTV 3'). The cDNA was used as a template for PCR amplification using AUAP_3'RACE primer (5' GGC CAC GCG TCG ACT AGT AC 3') and universal CRR primer CRR_ppt1F (5' CAA GAA GGG GAG GAT GAT G 3'). To make PCR more specific and sensitive, the PCR products were re-amplified using AUAP_3'RACE primer in combination with primer specific for CRR1 subfamily (CRR1_1F; 5' TGA TGA GGA CAT CCC TTC C 3'). Products of this reaction were resolved on agarose gel and only DNA from the prominent band was used for cloning.

text: four weeks old plant

text: leaf

text: Oryza sativa Japonica Group


text: University of Wisconsin

text: live

text: Generic

text: Nipponbare

text: The library contains 3'RACE products derived from CRR (Centromeric Retrotransposon of Rice). Total RNA was reversely transcribed into cDNA using 3'RACE_oligoT primer (5' GGC CAC GCG TCG ACT AGT ACT TTT TTT TTT TTT TTT TTV 3'). The cDNA was used as a template for PCR amplification using AUAP_3'RACE primer (5' GGC CAC GCG TCG ACT AGT AC 3') and universal CRR primer CRR_ppt1F (5' CAA GAA GGG GAG GAT GAT G 3'). To make PCR more specific and sensitive, the PCR products were re-amplified using AUAP_3'RACE primer in combination with primer specific for CRR2 subfamily (CRR2_1F; 5' TGA TGA GGA CAT CAA CAC CA 3'). Products of this reaction were resolved on agarose gel and only DNA from the prominent band was used for cloning.

text: four weeks old plant

text: leaf

text: Oryza sativa Japonica Group


text: University of Wisconsin

text: live

text: Generic

text: Nipponbare

text: The library contains 3'RACE products derived from CRR (Centromeric Retrotransposon of Rice). Total RNA was reversely transcribed into cDNA using 3'RACE_oligoT primer (5' GGC CAC GCG TCG ACT AGT ACT TTT TTT TTT TTT TTT TTV 3'). The cDNA was used as a template for PCR amplification using AUAP_3'RACE primer (5' GGC CAC GCG TCG ACT AGT AC 3') and universal CRR primer CRR_ppt1F (5' CAA GAA GGG GAG GAT GAT G 3'). To make PCR more specific and sensitive, the PCR products were re-amplified using AUAP_3'RACE primer in combination with primer specific for NOACRR1 subfamily (NOACRR1_1F; 5' NTG ATG TGA CCA TGG CTA CTA 3'). Products of this reaction were resolved on agarose gel and only DNA from the prominent band was used for cloning.

text: four weeks old plant

text: leaf

text: Oryza sativa Japonica Group


text: University of Wisconsin

text: live

text: Generic

text: Nipponbare

text: The library contains 3'RACE products derived from CRR (Centromeric Retrotransposon of Rice). Total RNA was reversely transcribed into cDNA using 3'RACE_oligoT primer (5' GGC CAC GCG TCG ACT AGT ACT TTT TTT TTT TTT TTT TTV 3'). The cDNA was used as a template for PCR amplification using AUAP_3'RACE primer (5' GGC CAC GCG TCG ACT AGT AC 3') and primer specific for CRR2 subfamily (CRR2_1F; 5' TGA TGA GGA CAT CAA CAC CA 3'). Products of this reaction were resolved on agarose gel and only DNA from the prominent band was used for cloning.

text: four weeks old plant

text: leaf

text: Oryza sativa Japonica Group


text: University of Wisconsin

text: live

text: Generic

text: Nipponbare

text: The library contains 3'RACE products derived from CRR (Centromeric Retrotransposon of Rice). Total RNA was reversely transcribed into cDNA using 3'RACE_oligoT primer (5' GGC CAC GCG TCG ACT AGT ACT TTT TTT TTT TTT TTT TTV 3'). The cDNA was used as a template for PCR amplification using AUAP_3'RACE primer (5' GGC CAC GCG TCG ACT AGT AC 3') and primer specific for NOACRR1 subfamily (NOACRR1_1F; 5' NTG ATG TGA CCA TGG CTA CTA 3'). Products of this reaction were resolved on agarose gel and only DNA from the prominent band was used for cloning.

text: four weeks old plant

text: leaf

text: Oryza sativa Japonica Group


text: University of Wisconsin

text: live

text: Generic

text: Nipponbare

text: The library contains RT-PCR products derived from CRR (Centromeric Retrotransposon of Rice). Total RNA was reversely transcribed into cDNA using random priming method. The cDNA was used as a template for PCR amplification using CRR-specific primers (NOACRR1_6F: 5' GCT CGC GTC TCT AGT ACG TT 3' and NOACRR1_7Rb: 5' TTT TCT ACT GCT AGC TCC CAA T 3').

text: four weeks old plant

text: leaf

text: Oryza sativa Japonica Group


text: University of Wisconsin

text: live

text: Generic

text: Nipponbare

text: The library contains 5'RACE products derived from CRR (Centromeric Retrotransposon of Rice). The ligase mediated 5'RACE was done using the GeneRACER kit (Invitrogen) following protocol described in the instruction manual. Total RNA was first dephosphorylated with calf intestine phosphatase to prevent ligation of non-mRNA and truncated mRNA. The dephosphorylated RNA was treated with tobacco acid pyrophosphatase to remove cap from the 5' ends of mRNA and then ligated to GeneRacer RNA oligo (5' CGA CUG GAG CAC GAG GAC ACU GAC AUG GAC UGA AGG AGU AGA AA 3'). The reverse transcription was done using random priming method. The reversely transcribed RNA was used as a template for PCR amplification using RACER_PN1 (5' GGA GCA CGA GGA CAC TGA 3') and CRR_pbs1R (5' GCT CTG ATA CCA NNT GAT G 3'. Products of this reaction were used as a template for PCR using RACER_PN2 (5' CAC TGA CAT GGA CTG AAG GA 3') and CRR1_2R (5' CGA TCT TTC GAT GAG AGG AG 3') primers. Products of this reaction were resolved on agarose gel and only DNA from the band I was used for cloning.

text: four weeks old plant

text: leaf

text: Oryza sativa Japonica Group


text: University of Wisconsin

text: live

text: Generic

text: Nipponbare

text: The library contains 5'RACE products derived from CRR (Centromeric Retrotransposon of Rice). The ligase mediated 5'RACE was done using the GeneRACER kit (Invitrogen) following protocol described in the instruction manual. Total RNA was first dephosphorylated with calf intestine phosphatase to prevent ligation of non-mRNA and truncated mRNA. The dephosphorylated RNA was treated with tobacco acid pyrophosphatase to remove cap from the 5' ends of mRNA and then ligated to GeneRacer RNA oligo (5' CGA CUG GAG CAC GAG GAC ACU GAC AUG GAC UGA AGG AGU AGA AA 3'). The reverse transcription was done using random priming method. The reversely transcribed RNA was used as a template for PCR amplification using RACER_PN1 (5' GGA GCA CGA GGA CAC TGA 3') and CRR_pbs1R (5' GCT CTG ATA CCA NNT GAT G 3'. Products of this reaction were used as a template for PCR using RACER_PN2 (5' CAC TGA CAT GGA CTG AAG GA 3') and CRR1_2R (5' CGA TCT TTC GAT GAG AGG AG 3') primers. Products of this reaction were resolved on agarose gel and only DNA from the band II was used for cloning.

text: four weeks old plant

text: leaf

text: Oryza sativa Japonica Group


text: University of Wisconsin

text: live

text: Generic

text: Nipponbare

text: The library contains 5'RACE products derived from CRR (Centromeric Retrotransposon of Rice). The ligase mediated 5'RACE was done using the GeneRACER kit (Invitrogen) following protocol described in the instruction manual. Total RNA was first dephosphorylated with calf intestine phosphatase to prevent ligation of non-mRNA and truncated mRNA. The dephosphorylated RNA was treated with tobacco acid pyrophosphatase to remove cap from the 5' ends of mRNA and then ligated to GeneRacer RNA oligo (5' CGA CUG GAG CAC GAG GAC ACU GAC AUG GAC UGA AGG AGU AGA AA 3'). The reverse transcription was done using random priming method. The reversely transcribed RNA was used as a template for PCR amplification using RACER_PN1 (5' GGA GCA CGA GGA CAC TGA 3') and CRR_pbs1R (5' GCT CTG ATA CCA NNT GAT G 3'. Products of this reaction were used as a template for PCR using RACER_PN2 (5' CAC TGA CAT GGA CTG AAG GA 3') and CRR2_2R (5' GAT CTT TCG ATG AGA GGT AAG G 3') primers. Products of this reaction were resolved on agarose gel and only DNA from the band I was used for cloning.

text: four weeks old plant

text: leaf

text: Oryza sativa Japonica Group


text: University of Wisconsin

text: live

text: Generic

text: Nipponbare

text: The library contains 5'RACE products derived from CRR (Centromeric Retrotransposon of Rice). The ligase mediated 5'RACE was done using the GeneRACER kit (Invitrogen) following protocol described in the instruction manual. Total RNA was first dephosphorylated with calf intestine phosphatase to prevent ligation of non-mRNA and truncated mRNA. The dephosphorylated RNA was treated with tobacco acid pyrophosphatase to remove cap from the 5' ends of mRNA and then ligated to GeneRacer RNA oligo (5' CGA CUG GAG CAC GAG GAC ACU GAC AUG GAC UGA AGG AGU AGA AA 3'). The reverse transcription was done using random priming method. The reversely transcribed RNA was used as a template for PCR amplification using RACER_PN1 (5' GGA GCA CGA GGA CAC TGA 3') and CRR_pbs1R (5' GCT CTG ATA CCA NNT GAT G 3'. Products of this reaction were used as a template for PCR using RACER_PN2 (5' CAC TGA CAT GGA CTG AAG GA 3') and CRR2_2R (5' GAT CTT TCG ATG AGA GGT AAG G 3') primers. Products of this reaction were resolved on agarose gel and only DNA from the band II was used for cloning.

text: four weeks old plant

text: leaf

text: Oryza sativa Japonica Group


text: University of Wisconsin

text: live

text: Generic

text: Nipponbare

text: The library contains 5'RACE products derived from CRR (Centromeric Retrotransposon of Rice). The ligase mediated 5'RACE was done using the GeneRACER kit (Invitrogen) following protocol described in the instruction manual. Total RNA was first dephosphorylated with calf intestine phosphatase to prevent ligation of non-mRNA and truncated mRNA. The dephosphorylated RNA was treated with tobacco acid pyrophosphatase to remove cap from the 5' ends of mRNA and then ligated to GeneRacer RNA oligo (5' CGA CUG GAG CAC GAG GAC ACU GAC AUG GAC UGA AGG AGU AGA AA 3'). The reverse transcription was done using random priming method. The reversely transcribed RNA was used as a template for PCR amplification using RACER_PN1 (5' GGA GCA CGA GGA CAC TGA 3') and CRR_pbs1R (5' GCT CTG ATA CCA NNT GAT G 3'. Products of this reaction were used as a template for PCR using RACER_PN2 (5' CAC TGA CAT GGA CTG AAG GA 3') and NOACRR1_2R (5' GAT GAA CCC TGA GTT CCW CT 3') primers. Products of this reaction were resolved on agarose gel and only DNA from the band I was used for cloning.

text: four weeks old plant

text: leaf

text: Oryza sativa Japonica Group


text: University of Wisconsin

text: live

text: Generic

text: Nipponbare

text: The library contains 5'RACE products derived from CRR (Centromeric Retrotransposon of Rice). The ligase mediated 5'RACE was done using the GeneRACER kit (Invitrogen) following protocol described in the instruction manual. Total RNA was first dephosphorylated with calf intestine phosphatase to prevent ligation of non-mRNA and truncated mRNA. The dephosphorylated RNA was treated with tobacco acid pyrophosphatase to remove cap from the 5' ends of mRNA and then ligated to GeneRacer RNA oligo (5' CGA CUG GAG CAC GAG GAC ACU GAC AUG GAC UGA AGG AGU AGA AA 3'). The reverse transcription was done using random priming method. The reversely transcribed RNA was used as a template for PCR amplification using RACER_PN1 (5' GGA GCA CGA GGA CAC TGA 3') and CRR_pbs1R (5' GCT CTG ATA CCA NNT GAT G 3'. Products of this reaction were used as a template for PCR using RACER_PN2 (5' CAC TGA CAT GGA CTG AAG GA 3') and NOACRR1_2R (5' GAT GAA CCC TGA GTT CCW CT 3') primers. Products of this reaction were resolved on agarose gel and only DNA from the band II was used for cloning.

text: four weeks old plant

text: leaf

text: Oryza sativa Japonica Group


text: University of Wisconsin

text: live

text: Generic

text: Nipponbare

text: The library contains 5'RACE products derived from CRR (Centromeric Retrotransposon of Rice). The ligase mediated 5'RACE was done using the GeneRACER kit (Invitrogen) following protocol described in the instruction manual. Total RNA was first dephosphorylated with calf intestine phosphatase to prevent ligation of non-mRNA and truncated mRNA. The dephosphorylated RNA was treated with tobacco acid pyrophosphatase to remove cap from the 5' ends of mRNA and then ligated to GeneRacer RNA oligo (5' CGA CUG GAG CAC GAG GAC ACU GAC AUG GAC UGA AGG AGU AGA AA 3'). The reverse transcription was done using random priming method. The reversely transcribed RNA was used as a template for PCR amplification using RACER_PN1 (5' GGA GCA CGA GGA CAC TGA 3') and CRR_pbs1R (5' GCT CTG ATA CCA NNT GAT G 3'. Products of this reaction were used as a template for PCR using RACER_PN2 (5' CAC TGA CAT GGA CTG AAG GA 3') and NOACRR1_2R (5' GAT GAA CCC TGA GTT CCW CT 3') primers. Products of this reaction were resolved on agarose gel and only DNA from the band III was used for cloning.

text: four weeks old plant

text: leaf

text: Oryza sativa Japonica Group


text: University of Wisconsin

text: live

text: Generic

text: Nipponbare

text: The library contains RT-PCR products derived from CRR (Centromeric Retrotransposon of Rice). Total RNA was reversely transcribed into cDNA using random priming method. The cDNA was used as a template for PCR amplification using CRR-specific primers (CRR1_8R: 5' CAC ACA TAG CTG AAA GTA GAC GAC 3' and CRR1/2_7F: 5' GGA TTG AGC ACC AAA TTG A 3' + 5' GCA TTG AGC ATC AAA TCG A 3').

text: flowering

text: panicle

text: Oryza sativa Japonica Group


text: University of Wisconsin

text: live

text: Generic

text: Nipponbare

text: The library contains RT-PCR products derived from CRR (Centromeric Retrotransposon of Rice). Total RNA was reversely transcribed into cDNA using random priming method. The cDNA was used as a template for PCR amplification using CRR-specific primers (CRR1/2_5R 5' CGA AGA ACA AGC AAG AAC AAG 3' and CRR1/2_7F: 5' GGA TTG AGC ACC AAA TTG A 3' + 5' GCA TTG AGC ATC AAA TCG A 3').

text: flowering

text: panicle

text: Oryza sativa Japonica Group


text: Fujian Agriculture and Forestry University

text: live

text: Generic

text: Rice seedings with 4-6 leaves were inoculated with sheath blight isolate (short and thin toothpicks incubated after 3 day ). Seedings from 4, 12, 24 and 48 hours after inoculation were collected and mixed equally for SSH cDNA library construction

text: seedings with 4-6 leaves

text: Oryza sativa Indica Group

text: Seedlings

text: SSH cDNA library during infection by Rhizoctonia solani

text: Plasmid

text: pGEMT-T easy vector(promega)


text: National Institute of Agrobiological Sciences

text: live

text: Generic

text: Nipponbare

text: Oryza sativa Japonica Group

text: etiolated shoot

text: Oryza sativa (japonica cultivar-group) etiolated shoot


text: live

text: Generic

text: Nipponbare

text: Oryza sativa Japonica Group

text: green shoot

text: Oryza sativa (japonica cultivar-group) green shoot


text: live

text: Generic

text: Nipponbare

text: Oryza sativa Japonica Group

text: heat treated callus

text: Oryza sativa (japonica cultivar-group) heat treated callus


text: live

text: Generic

text: Nipponbare

text: Oryza sativa Japonica Group

text: UVC irradiated shoot

text: Oryza sativa (japonica cultivar-group) UVC irradiated shoot


text: live

text: Generic

text: Nipponbare

text: Oryza sativa Japonica Group

text: BAP treated callus

text: Oryza sativa (japonica cultivar-group) BAP treated callus


text: live

text: Generic

text: Nipponbare

text: Oryza sativa Japonica Group

text: mixed callus (Normalized Library)

text: Oryza sativa (japonica cultivar-group) mixed callus (Normalized Library)


text: live

text: Generic

text: Nipponbare

text: Oryza sativa Japonica Group

text: mixed shoot (Normalized Library)

text: Oryza sativa (japonica cultivar-group) mixed shoot (Normalized Library)


text: live

text: Generic

text: Nipponbare

text: Oryza sativa Japonica Group

text: NAA treated callus

text: Oryza sativa (japonica cultivar-group) NAA treated callus


text: live

text: Generic

text: Nipponbare

text: Oryza sativa Japonica Group

text: ABA: Abscic Acid treated callus

text: Oryza sativa (japonica cultivar-group) ABA: Abscic Acid treated callus


text: live

text: Generic

text: Nipponbare

text: Oryza sativa Japonica Group

text: Cd treated callus

text: Oryza sativa (japonica cultivar-group) Cd treated callus


text: live

text: Generic

text: Nipponbare

text: Oryza sativa Japonica Group

text: Panicles more than 5cm stage

text: Oryza sativa (japonica cultivar-group) Panicles more than 5cm stage


text: live

text: Generic

text: Nipponbare

text: Oryza sativa Japonica Group

text: Panicles less than 5cm stage

text: Oryza sativa (japonica cultivar-group) Panicles less than 5cm stage


text: live

text: Generic

text: Nipponbare

text: Oryza sativa Japonica Group

text: supermix

text: Oryza sativa (japonica cultivar-group) supermix


text: live

text: Generic

text: Nipponbare

text: Oryza sativa Japonica Group

text: Oryza sativa (japonica cultivar-group) Panicles more than 5cm stage and Panicles one day after flowering


text: live

text: Generic

text: Nipponbare

text: Oryza sativa Japonica Group

text: Oryza sativa (japonica cultivar-group) Panicles less than 5cm stage and Panicles two weeks after flowering


text: live

text: Generic

text: Nipponbare

text: Oryza sativa Japonica Group

text: Oryza sativa (japonica cultivar-group) Panicles mixture of one, two, three weeks after flowering


text: National Institute of Agrobiological Sciences

text: live

text: Generic

text: Nipponbare

text: Oryza sativa Japonica Group

text: shoot and callus

text: Oryza sativa (japonica cultivar-group) shoot and callus


text: live

text: Generic

text: Nipponbare

text: Oryza sativa Japonica Group

text: root of seedlings

text: Oryza sativa (japonica cultivar-group) root of seedlings


text: live

text: Generic

text: Nipponbare

text: Oryza sativa Japonica Group

text: Shoot and root of germinating seeds

text: Oryza sativa (japonica cultivar-group) Shoot and root of germinating seeds


text: live

text: Generic

text: Nipponbare

text: Oryza sativa Japonica Group

text: Oryza sativa (japonica cultivar-group) Panicles mixture of one, two, three weeks after flowering and supermix


text: live

text: Generic

text: Nipponbare

text: Oryza sativa Japonica Group

text: same as Lib 23(supermix)

text: Oryza sativa (japonica cultivar-group) same as Lib 23(supermix)


text: live

text: Generic

text: Nipponbare

text: Oryza sativa Japonica Group

text: flower

text: Oryza sativa (japonica cultivar-group) flower


text: live

text: Generic

text: Nipponbare

text: Oryza sativa Japonica Group

text: callus

text: Oryza sativa (japonica cultivar-group) callus


text: live

text: Generic

text: Nipponbare

text: Oryza sativa Japonica Group

text: cold treated callus

text: Oryza sativa (japonica cultivar-group) cold treated callus


text: live

text: Generic

text: Nipponbare

text: Oryza sativa Japonica Group

text: leaf of seedling

text: Oryza sativa (japonica cultivar-group) leaf of seedling


text: live

text: Generic

text: Nipponbare

text: Oryza sativa Japonica Group

text: 1 week on callus-induction medium seed

text: Oryza sativa (japonica cultivar-group) 1 week on callus-induction medium seed


text: live

text: Generic

text: Nipponbare

text: Oryza sativa Japonica Group

text: Oryza sativa (japonica cultivar-group) library (Kikuchi S)


text: live

text: Generic

text: Nipponbare

text: Oryza sativa Japonica Group

text: germinating seeds

text: Oryza sativa (japonica cultivar-group) germinating seeds


text: live

text: Generic

text: Nipponbare

text: 1mM ACC(1-aminocyclopropane- 1-carboxylate), after 24 hr

text: Oryza sativa Japonica Group

text: 4th leaf leaf

text: Oryza sativa (japonica cultivar-group) 4th leaf leaf 1mM ACC(1-aminocyclopropane-1-carboxylate)


text: live

text: Generic

text: Nipponbare

text: 3mM SA(salicylic acid) for 1 day

text: Oryza sativa Japonica Group

text: 4th leaf leaf

text: Oryza sativa (japonica cultivar-group) 4th leaf leaf 3mM SA(salicylic acid) for 1 day


text: live

text: Generic

text: Nipponbare

text: transferred to redifferentiation medium

text: Oryza sativa Japonica Group

text: 4 days after transferring callus

text: Oryza sativa (japonica cultivar-group) 4 days after transferring callus


text: National Institute of Agrobiological Sciences

text: live

text: Generic

text: Nipponbare

text: transferred to redifferentiation medium

text: Oryza sativa Japonica Group

text: 11 days after transferring callus

text: Oryza sativa (japonica cultivar-group) 11 days after transferring callus


text: live

text: Generic

text: Nipponbare

text: transferred to redifferentiation medium

text: Oryza sativa Japonica Group

text: 11 days after transferring callus

text: Oryza sativa (japonica cultivar-group) 14 days after transferring callus


text: live

text: Generic

text: Nipponbare

text: 100ppm ZnSO4 for 1week

text: Oryza sativa Japonica Group

text: callus

text: Oryza sativa (japonica cultivar-group) callus 100ppm ZnSO4 for 1week


text: live

text: Generic

text: Nipponbare

text: 200ppm MnSO4 for 1week

text: Oryza sativa Japonica Group

text: callus

text: Oryza sativa (japonica cultivar-group) callus 200ppm MnSO4 for 1week


text: live

text: Generic

text: Nipponbare

text: 2ppm ABA(abcisic acid)

text: Oryza sativa Japonica Group

text: callus

text: Oryza sativa (japonica cultivar-group) callus 2ppm ABA(abcisic acid)


text: live

text: Generic

text: Nipponbare

text: 2ppm BAP

text: Oryza sativa Japonica Group

text: callus

text: Oryza sativa (japonica cultivar-group) callus 2ppm BAP


text: live

text: Generic

text: Nipponbare

text: 2ppm NAA

text: Oryza sativa Japonica Group

text: callus

text: Oryza sativa (japonica cultivar-group) callus 2ppm NAA


text: live

text: Generic

text: Nipponbare

text: 50ppm CdCl2

text: Oryza sativa Japonica Group

text: callus

text: Oryza sativa (japonica cultivar-group) callus 50ppm CdCl2


text: live

text: Generic

text: Nipponbare

text: 50ppm CuSO4 for 1 week

text: Oryza sativa Japonica Group

text: callus

text: Oryza sativa (japonica cultivar-group) callus 50ppm CuSO4 for 1 week


text: live

text: Generic

text: Nipponbare

text: high temperature at 45 degree, 2hr.

text: Oryza sativa Japonica Group

text: callus

text: Oryza sativa (japonica cultivar-group) callus high temperature at 45 degree, 2hr.


text: live

text: Generic

text: Nipponbare

text: high temperature at 45 degree, 30min

text: Oryza sativa Japonica Group

text: callus

text: Oryza sativa (japonica cultivar-group) callus high temperature at 45 degree, 30min


text: live

text: Generic

text: Nipponbare

text: low temperature at 6 degree

text: Oryza sativa Japonica Group

text: callus

text: Oryza sativa (japonica cultivar-group) callus low temperature at 6 degree


text: live

text: Generic

text: Nipponbare

text: Oryza sativa Japonica Group

text: callus

text: Oryza sativa (japonica cultivar-group) callus suspension-cultured cells treated with elicitor


text: live

text: Generic

text: Nipponbare

text: Oryza sativa Japonica Group

text: callus

text: Oryza sativa (japonica cultivar-group) callus UVB iradiated callus, 24hr after treatment


text: live

text: Generic

text: Nipponbare

text: Oryza sativa Japonica Group

text: callus

text: Oryza sativa (japonica cultivar-group) callus UVB iradiated callus, immid after treatment


text: live

text: Generic

text: Nipponbare

text: Oryza sativa Japonica Group

text: seed

text: Oryza sativa (japonica cultivar-group) seed


text: National Institute of Agrobiological Sciences

text: live

text: Generic

text: Nipponbare

text: 10mM H2O2 (10min), 24hr after treatment

text: Oryza sativa Japonica Group

text: leaf of seedling

text: Oryza sativa (japonica cultivar-group) leaf of seedling 10mM H2O2 (10min), 24hr after treatment


text: live

text: Generic

text: Nipponbare

text: 50ppm CuSO4 for 3 days

text: Oryza sativa Japonica Group

text: leaf of seedling

text: Oryza sativa (japonica cultivar-group) leaf of seedling 50ppm CuSO4 for 3 days


text: live

text: Generic

text: Nipponbare

text: gamma-irradiated (4min), after 6hr

text: Oryza sativa Japonica Group

text: leaf of seedling

text: Oryza sativa (japonica cultivar-group) leaf of seedling gamma-irradiated (4min), after 6hr


text: live

text: Generic

text: Nipponbare

text: gamma-irradiated (9.5min), after 10hr

text: Oryza sativa Japonica Group

text: leaf of seedling

text: Oryza sativa (japonica cultivar-group) leaf of seedling gamma-irradiated (9.5min), after 10hr


text: live

text: Generic

text: Nipponbare

text: Oryza sativa Japonica Group

text: leaf of seedling

text: Oryza sativa (japonica cultivar-group) leaf of seedling gamma-irradiated(45Gy), immid after irradiation


text: live

text: Generic

text: Nipponbare

text: irradiated with UVB

text: Oryza sativa Japonica Group

text: leaf of seedling

text: Oryza sativa (japonica cultivar-group) leaf of seedling irradiated with UVB


text: live

text: Generic

text: Nipponbare

text: irradiated with UVC [0.7min ( 226.8J/m2)]

text: Oryza sativa Japonica Group

text: leaf of seedling

text: Oryza sativa (japonica cultivar-group) leaf of seedling irradiated with UVC [0.7 min ( 226.8J/m2)]


text: live

text: Generic

text: Nipponbare

text: Oryza sativa Japonica Group

text: root of seedling

text: Oryza sativa (japonica cultivar-group) root of seedling irradiated with UVB [2.4min (547.2J/m2)]


text: live

text: Generic

text: Nipponbare

text: Oryza sativa Japonica Group

text: shoot

text: Oryza sativa (japonica cultivar-group) shoot


text: live

text: Generic

text: Nipponbare

text: Oryza sativa Japonica Group

text: Oryza sativa (japonica cultivar-group) Panicles mixture of one, two, three weeks after flowering and root of seedlings


text: live

text: Generic

text: Nipponbare

text: Oryza sativa Japonica Group

text: same as Lib 33(supermix)

text: Oryza sativa (japonica cultivar-group) same as Lib 33(supermix)


text: live

text: Generic

text: Nipponbare

text: Oryza sativa Japonica Group

text: Oryza sativa (japonica cultivar-group) mixed callus (Normalized Library) and mixed shoot (Normalized Library)


text: live

text: Generic

text: Nipponbare

text: Oryza sativa Japonica Group

text: 4 nuclei stage pistil

text: Oryza sativa (japonica cultivar-group) 4 nuclei stage pistil


text: live

text: Generic

text: Nipponbare

text: probenazole

text: Oryza sativa Japonica Group

text: 9 leaf-stage leaf

text: Oryza sativa (japonica cultivar-group) 9 leaf-stage leaf


text: live

text: Generic

text: Nipponbare

text: Oryza sativa Japonica Group

text: 1 nuclei stage anther

text: Oryza sativa (japonica cultivar-group) 1 nuclei stage anther


text: National Institute of Agrobiological Sciences

text: live

text: Generic

text: Nipponbare

text: Oryza sativa Japonica Group

text: 2 nuclei stage anther

text: Oryza sativa (japonica cultivar-group) 2 nuclei stage anther


text: live

text: Generic

text: Nipponbare

text: Oryza sativa Japonica Group

text: after pollination embryo

text: Oryza sativa (japonica cultivar-group) after pollination embryo


text: live

text: Generic

text: Nipponbare

text: Oryza sativa Japonica Group

text: 5 days after pollination pistil

text: Oryza sativa (japonica cultivar-group) 5 days after pollination pistil


text: live

text: Generic

text: Nipponbare

text: Oryza sativa Japonica Group

text: 1 day after pollination pistil

text: Oryza sativa (japonica cultivar-group) 1 day after pollination pistil


text: live

text: Generic

text: Nipponbare

text: Oryza sativa Japonica Group

text: 2 nuclei stage pistil

text: Oryza sativa (japonica cultivar-group) 2 nuclei stage pistil


text: live

text: Generic

text: Nipponbare

text: Oryza sativa Japonica Group

text: 1 nuclei stage pistil

text: Oryza sativa (japonica cultivar-group) 1 nuclei stage pistil


text: live

text: Generic

text: Nipponbare

text: Oryza sativa Japonica Group

text: 4 nuclei stage anther

text: Oryza sativa (japonica cultivar-group) 4 nuclei stage anther


text: live

text: Generic

text: Nipponbare

text: Oryza sativa Japonica Group

text: 2 weeks seedlings root of seedling

text: Oryza sativa (japonica cultivar-group) 2 weeks seedlings root of seedling UVB irradiated (2.4min), 24hr after treatment


text: live

text: Generic

text: Nipponbare

text: Oryza sativa Japonica Group

text: 2 weeks seedlings root of seedling

text: Oryza sativa (japonica cultivar-group) 2 weeks seedlings root of seedling UVC irradiated (0.7min), 48hr after treatment


text: live

text: Generic

text: Nipponbare

text: 0.1M NaCl for 3 days

text: Oryza sativa Japonica Group

text: root of seedling

text: Oryza sativa (japonica cultivar-group) root of seedling 0.1M NaCl for 3 days


text: live

text: Generic

text: Nipponbare

text: 10mM H2O2 (10min), 24hr after treatment

text: Oryza sativa Japonica Group

text: root of seedling

text: Oryza sativa (japonica cultivar-group) root of seedling 10mM H2O2 (10min), 24hr after treatment


text: live

text: Generic

text: Nipponbare

text: 2ppm BAP(benzyl amino purine)

text: Oryza sativa Japonica Group

text: root of seedling

text: Oryza sativa (japonica cultivar-group) root of seedling 2ppm BAP(benzyl amino purine)


text: live

text: Generic

text: Nipponbare

text: 2ppm NAA(naphthalene acetic acid)

text: Oryza sativa Japonica Group

text: root of seedling

text: Oryza sativa (japonica cultivar-group) root of seedling 2ppm NAA(naphthalene acetic acid)


text: live

text: Generic

text: Nipponbare

text: 50ppm CuSO4 for 3days

text: Oryza sativa Japonica Group

text: root of seedling

text: Oryza sativa (japonica cultivar-group) root of seedling 50ppm CuSO4 for 3days


text: live

text: Generic

text: Nipponbare

text: 6ppm CdCl2 for 3 days

text: Oryza sativa Japonica Group

text: root of seedling

text: Oryza sativa (japonica cultivar-group) root of seedling 6ppm CdCl2 for 3 days


text: live

text: Generic

text: Nipponbare

text: gamma-irradiated (4min), after 10hr

text: Oryza sativa Japonica Group

text: root of seedling

text: Oryza sativa (japonica cultivar-group) root of seedling gamma-irradiated (4min), after 10hr


text: National Institute of Agrobiological Sciences

text: live

text: Generic

text: Nipponbare

text: gamma-irradiated (4min), after 6hr

text: Oryza sativa Japonica Group

text: root of seedling

text: Oryza sativa (japonica cultivar-group) root of seedling gamma-irradiated (4min), after 6hr


text: live

text: Generic

text: Nipponbare

text: amma-irradiated (9.5min), after 10hr

text: Oryza sativa Japonica Group

text: root of seedling

text: Oryza sativa (japonica cultivar-group) root of seedling gamma-irradiated (9.5min), after 10hr


text: live

text: Generic

text: Nipponbare

text: Oryza sativa Japonica Group

text: root of seedling

text: Oryza sativa (japonica cultivar-group) root of seedling gamma-irradiated(45Gy), immid after irradiation


text: live

text: Generic

text: Nipponbare

text: gamma-irradiated, after 24hr

text: Oryza sativa Japonica Group

text: root of seedling

text: Oryza sativa (japonica cultivar-group) root of seedling gamma-irradiated, after 24hr


text: live

text: Generic

text: Nipponbare

text: in anaerobic condition for 3 days

text: Oryza sativa Japonica Group

text: root of seedling

text: Oryza sativa (japonica cultivar-group) root of seedling in anaerobic condition for 3 days


text: live

text: Generic

text: Nipponbare

text: Oryza sativa Japonica Group

text: 2 weeks after flowering ear

text: Oryza sativa (japonica cultivar-group) 2 weeks after flowering ear


text: live

text: Generic

text: Nipponbare

text: Oryza sativa Japonica Group

text: 1 week after flowering ear

text: Oryza sativa (japonica cultivar-group) 1 week after flowering ear


text: live

text: Generic

text: Nipponbare

text: Oryza sativa Japonica Group

text: 40 days after pollination pistil

text: Oryza sativa (japonica cultivar-group) 40 days after pollination pistil


text: live

text: Generic

text: Nipponbare

text: Oryza sativa Japonica Group

text: 10 days after pollination pistil

text: Oryza sativa (japonica cultivar-group) 10 days after pollination pistil


text: live

text: Generic

text: Nipponbare

text: Oryza sativa Japonica Group

text: 20 days after pollination pistil

text: Oryza sativa (japonica cultivar-group) 20 days after pollination pistil


text: live

text: Generic

text: Nipponbare

text: Oryza sativa Japonica Group

text: 3rd week immature panicle

text: Oryza sativa (japonica cultivar-group) 3rd week immature panicle


text: live

text: Generic

text: Nipponbare

text: Oryza sativa Japonica Group

text: 4.5 leaf-stage leaf

text: Oryza sativa (japonica cultivar-group) 4.5 leaf-stage leaf


text: live

text: Generic

text: Nipponbare

text: Oryza sativa Japonica Group

text: 35 days after pollination pistil

text: Oryza sativa (japonica cultivar-group) 35 days after pollination pistil


text: live

text: Generic

text: Nipponbare

text: Oryza sativa Japonica Group

text: young ear

text: Oryza sativa (japonica cultivar-group) young ear


text: live

text: Generic

text: Nipponbare

text: Oryza sativa Japonica Group

text: 25 days after pollination pistil

text: Oryza sativa (japonica cultivar-group) 25 days after pollination pistil


text: live

text: Generic

text: Nipponbare

text: Oryza sativa Japonica Group

text: whole body

text: Oryza sativa (japonica cultivar-group) whole body 1mM ACC(1-aminocyclopropane-1-carboxylate)


text: National Institute of Agrobiological Sciences

text: live

text: Generic

text: Nipponbare

text: 3mM SA(salicylic acid) for 1day

text: Oryza sativa Japonica Group

text: whole body

text: Oryza sativa (japonica cultivar-group) whole body 3mM SA(salicylic acid) for 1day


text: live

text: Generic

text: Nipponbare

text: Oryza sativa Japonica Group

text: 2nd week immature panicle

text: Oryza sativa (japonica cultivar-group) 2nd week immature panicle


text: live

text: Generic

text: Nipponbare

text: Oryza sativa Japonica Group

text: 15 days after pollination pistil

text: Oryza sativa (japonica cultivar-group) 15 days after pollination pistil


text: live

text: Generic

text: Nipponbare

text: Oryza sativa Japonica Group

text: 3 weeks after flowering ear

text: Oryza sativa (japonica cultivar-group) 3 weeks after flowering ear


text: live

text: Generic

text: Nipponbare

text: Oryza sativa Japonica Group

text: 30 days after pollination pistil

text: Oryza sativa (japonica cultivar-group) 30 days after pollination pistil


text: live

text: Generic

text: Nipponbare

text: Oryza sativa Japonica Group

text: Panicles two weeks after flowering

text: Oryza sativa (japonica cultivar-group) Panicles two weeks after flowering


text: live

text: Generic

text: Nipponbare

text: Oryza sativa Japonica Group

text: Panicles one day after flowering

text: Oryza sativa (japonica cultivar-group) Panicles one day after flowering


text: live

text: Generic

text: Oryza sativa Indica Group

text: anther

text: Oryza sativa (indica cultivar-group) anther


text: University of Wisconsin

text: live

text: Generic

text: Nipponbare

text: The library contains 3'RACE products derived from rice centromeric satellite CentO. Total RNA was reversely transcribed into cDNA using 3'RACE_oligoT primer (5' GGC CAC GCG TCG ACT AGT ACT TTT TTT TTT TTT TTT TTV 3'). The cDNA was used as a template for PCR amplification using AUAP_3'RACE primer (5' GGC CAC GCG TCG ACT AGT AC 3') and CentO specific primer CentO_R1 (5' AAA CAT GNT TTT TGG ACA TAT TG 3').

text: four weeks old plant

text: leaf

text: Oryza sativa Japonica Group


text: University of Wisconsin

text: live

text: Generic

text: Nipponbare

text: The library contains 3'RACE products derived from rice centromeric satellite CentO. Total RNA was reversely transcribed into cDNA using 3'RACE_oligoT primer (5' GGC CAC GCG TCG ACT AGT ACT TTT TTT TTT TTT TTT TTV 3'). The cDNA was used as a template for PCR amplification using AUAP_3'RACE primer (5' GGC CAC GCG TCG ACT AGT AC 3') and CentO specific primer CentO_L+ (5' GNT TTT TGG ACA TAT TGG AGT G 3').

text: four weeks old plant

text: leaf

text: Oryza sativa Japonica Group


text: University of Wisconsin

text: live

text: Generic

text: Nipponbare

text: The library contains 3'RACE products derived from rice centromeric satellite CentO. Total RNA was reversely transcribed into cDNA using 3'RACE_oligoT primer (5' GGC CAC GCG TCG ACT AGT ACT TTT TTT TTT TTT TTT TTV 3'). The cDNA was used as a template for PCR amplification using AUAP_3'RACE primer (5' GGC CAC GCG TCG ACT AGT AC 3') and CentO specific primer CentO_R2 (5' ANT GTA TTG GGT GCG TTC G 3').

text: flowering

text: panicle

text: Oryza sativa Japonica Group


text: University of Wisconsin

text: live

text: Generic

text: Nipponbare

text: flowering

text: panicle

text: Oryza sativa Japonica Group


text: University of Wisconsin

text: live

text: Generic

text: Nipponbare

text: The library contains 3'RACE products derived from rice centromeric satellite CentO. Total RNA was reversely transcribed into cDNA using 3'RACE_oligoT primer (5' GGC CAC GCG TCG ACT AGT ACT TTT TTT TTT TTT TTT TTV 3'). The cDNA was used as a template for PCR amplification using AUAP_3'RACE primer (5' GGC CAC GCG TCG ACT AGT AC 3') and CentO specific primer CentO_F1 (5' CAA TAT GTC CAA AAA NCA TGT TT 3').

text: four weeks old plant

text: root

text: Oryza sativa Japonica Group


text: University of Wisconsin

text: live

text: Generic

text: Nipponbare

text: The library contains RTPCR products derived from rice centromeric satellite CentO. Total RNA was reversely transcribed into cDNA using a mix of three CentO-specific primers designed from a reverse strand (CentO_L+: 5' GNT TTT TGG ACA TAT TGG AGT G 3', CentO_R1: 5' AAA CAT GNT TTT TGG ACA TAT TG 3', and CentO_R2: 5' ANT GTA TTG GGT GCG TTC G 3'). The cDNA was used as a template for PCR amplification using CentO_U (5' TCA TGT TTT GGT GCT TTT TG 3') and CentO_L+ (5' GNT TTT TGG ACA TAT TGG AGT G 3') primers.

text: four weeks old plant

text: root

text: Oryza sativa Japonica Group


text: University of Wisconsin

text: live

text: Generic

text: Nipponbare

text: four weeks old plant

text: leaf

text: Oryza sativa Japonica Group


text: University of Wisconsin

text: live

text: Generic

text: Nipponbare

text: flowering

text: panicle

text: Oryza sativa Japonica Group


text: University of Wisconsin

text: live

text: Generic

text: Nipponbare

text: The library contains RTPCR products derived from rice centromeric satellite CentO. Total RNA was reversely transcribed into cDNA using a mix of three CentO-specific primers designed from a forward strand (CentO_U 5' TCA TGT TTT GGT GCT TTT TG 3', CentO_F1 5' CAA TAT GTC CAA AAA NCA TGT TT 3', and CentO_F2 5' CGA ACG CAC CCA ATA CAN T 3'). The cDNA was used as a template for PCR amplification using CentO_U (5' TCA TGT TTT GGT GCT TTT TG 3') and CentO_L+ (5' GNT TTT TGG ACA TAT TGG AGT G 3') primers.

text: four weeks old plant

text: root

text: Oryza sativa Japonica Group


text: University of Wisconsin

text: live

text: Generic

text: Nipponbare

text: four weeks old plant

text: leaf

text: Oryza sativa Japonica Group


text: University of Wisconsin

text: live

text: Generic

text: Nipponbare

text: flowering

text: panicle

text: Oryza sativa Japonica Group


text: University of Wisconsin

text: live

text: Generic

text: Nipponbare

text: The library contains RTPCR products derived from rice centromeric satellite CentO. Total RNA was reversely transcribed into cDNA using a mix of three CentO-specific primers designed from a reverse strand (CentO_L+: 5' GNT TTT TGG ACA TAT TGG AGT G 3', CentO_R1: 5' AAA CAT GNT TTT TGG ACA TAT TG 3', and CentO_R2: 5' ANT GTA TTG GGT GCG TTC G 3'). The cDNA was used as a template for PCR amplification using CentO_F1 (5' CAA TAT GTC CAA AAA NCA TGT TT 3') and CentO_R2 (5' ANT GTA TTG GGT GCG TTC G 3') primers.

text: four weeks old plant

text: root

text: Oryza sativa Japonica Group


text: University of Wisconsin

text: live

text: Generic

text: Nipponbare

text: four weeks old plant

text: leaf

text: Oryza sativa Japonica Group


text: University of Wisconsin

text: live

text: Generic

text: Nipponbare

text: flowering

text: panicle

text: Oryza sativa Japonica Group


text: University of Wisconsin

text: live

text: Generic

text: Nipponbare

text: The library contains RTPCR products derived from rice centromeric satellite CentO. Total RNA was reversely transcribed into cDNA using a mix of three CentO-specific primers designed from a forward strand (CentO_U 5' TCA TGT TTT GGT GCT TTT TG 3', CentO_F1 5' CAA TAT GTC CAA AAA NCA TGT TT 3', and CentO_F2 5' CGA ACG CAC CCA ATA CAN T 3'). The cDNA was used as a template for PCR amplification using CentO_F1 (5' CAA TAT GTC CAA AAA NCA TGT TT 3') and CentO_R2 (5' ANT GTA TTG GGT GCG TTC G 3') primers.

text: four weeks old plant

text: root

text: Oryza sativa Japonica Group


text: University of Wisconsin

text: live

text: Generic

text: Nipponbare

text: four weeks old plant

text: leaf

text: Oryza sativa Japonica Group


text: University of Wisconsin

text: live

text: Generic

text: Nipponbare

text: flowering

text: panicle

text: Oryza sativa Japonica Group