text: ARC Seibersdorf Research GesmbH

text: live

text: Generic

text: Ipomoea batata (Lam) L.; Smart cDNA library construction kit; Clontech: PT3000-1

text: Lambda

text: Ipomoea batatas

text: Sfi A

text: Sfi B

text: Leaf

text: Sweetpotato cDNA library

text: Phage vector


status: PUBLIC

characteristic: External Id

text: Plant Biotechnology Institute, National Research Council Canada

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: 15 days of pollination

tag: attribute

characteristic: dev-stage

text: Brassica napus

characteristic: organism

text: Westar

characteristic: strain

text: immature seeds

characteristic: tissue-type

text: Seed cDNA library

characteristic: title


text: Union Hospital of Fujian Medical University

text: live

text: Generic

text: Adult

text: Homo sapiens

text: EcoR 1

text: EcoR 1

text: Human leukemia cells

text: pT-adv

characteristic: vector


text: Wayne State University

text: live

text: Generic

text: cDNA sequences clone by immunoscreening a T7 bateriophage cDNA breast cancer library with breast cancer patient sera.

characteristic: description

text: Homo sapiens

text: T7 Breast Cancer Library


text: Washington University School of Medicine

text: live

text: Generic

text: The library was constructed by Claire Murphy and Dr. James McCarter at Washington University, St. Louis. Oligo(dT)-SL1 PCR based library. cDNA PCR products of size >400 nucleotides containing SL1 on the 5' end and oligo(dT) on the 3' end were non-directionally cloned into pCRII-TOPO(Invitrogen) following the Topo TA cloning protocol. Intestine RNA was provided by Dr. Douglas Jasmer of Washington State University (djasmer@vetmed.wsu.edu).

text: adult

characteristic: lab-host

text: Haemonchus contortus

text: EcoRI

text: EcoRI

text: intestine

text: Haemonchus contortus intestine SL1 TOPO v1

text: plasmid (kanamycin and ampicillin resistant)

text: pCRII-TOPO (Invitrogen)


text: live

text: Generic

text: The library was constructed by Claire Murphy and Dr. James McCarter at Washington University, St. Louis. Oligo(dT)-SL1 PCR based library. cDNA PCR products of size >400 nucleotides containing SL1 on the 5' end and oligo(dT) on the 3' end were non-directionally cloned into pCRII-TOPO(Invitrogen) following the Topo TA cloning protocol. Whole worm RNA was provided by Dr. Douglas Jasmer of Washington State University (djasmer@vetmed.wsu.edu).

text: Haemonchus contortus

text: EcoRI

text: EcoRI

text: whole organism

text: Haemonchus contortus whole worm SL1 TOPO v1

text: pCRII-TOPO (Invitrogen)


text: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry

text: live

text: Generic

text: testicle

characteristic: organ

text: Homo sapiens

text: male

characteristic: sex

text: human testicular parenchyma

text: Chimeric retrotranscripts expressed in human tissues


characteristic: External Id

text: University of Florida

text: live

text: Generic

text: Arachis hypogaea

text: seed coat

text: Arachis hypogaea seed coat


text: Institute of Chinese Military Medical Scienses

characteristic: INSDC center name

characteristic: INSDC secondary accession

text: live

text: Generic

characteristic: SRA accession

text: drug-resistance nasopharyngeal carcinoma cells

characteristic: cell type

characteristic: cell-line

text: Homo sapiens

text: Homo sapiens CNE-2


text: Department of Microbiology and Immunology, University of Maryland, Baltimore, School of Medicine

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: embryonic derived cell line DAE100

text: Subtracted cDNA subjected to nested PCR amplification and cloned into pCR4-TOPO vector.

text: Dermacentor andersoni

text: Subtracted Dermacentor andersoni cell line plasmid library


text: Institut fur Genetik, Universitat zu Koln

text: live

text: Generic

text: Uni-ZAP XR cDNA library (Stratagene) constructed by Reinhard Schroder (1995)

text: Mixed embryonic stages

characteristic: dev-stage

text: Tribolium castaneum

characteristic: organism

text: Wild type

characteristic: strain

text: Tribolium castaneum embryonic cDNA library

characteristic: title

text: Plasmid

text: pBluescript SK


text: live

text: Generic

text: The library was synthesized with modified SMART primers with dUTP at the end. After treatment with UDG the cDNA was cloned in pAMP1 vector by annealing.

characteristic: description

text: DH5alpha Ultra Max cells (Invitrogen)

characteristic: lab-host

text: Mus musculus

text: small intestinal epithelial progenitors

characteristic: tissue-type

text: SiEP

text: annealing vector

characteristic: vector


characteristic: External Id

text: Department of Genetics, The Hospital for Sick Children

characteristic: INSDC center name

text: live

text: Generic

text: Homo sapiens

text: SfiI

text: SFiI

text: Image cDNA Library

text: pBNR-LIV


text: UC Davis, Plant Pathology

text: live

text: Generic

text: Cabernet Sauvignon

characteristic: cultivar

text: CA23EI is a cDNA library of Cabernet Sauvignon leaves. The leaves were collected on July 25, 2001, in Napa Valley, California, and represent leaves in mid-season development. These leaves were verified to be infected with the bacterial pathogen, Xylella fastidiosa, based on a diagnostic assay using PCR and Xylella-specific primer pairs. The plants were asymptomatic at the time of collection, but later developed symptoms. cDNAs were made by oligo-dT priming and directionally cloned. 5'and 3' adaptors were used in cloning as follows: 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' and 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)NN-3'. Library was constructed using the Clontech Creator SMART kit and size-selected to contain the 0.5-3 kb size fraction.

text: Mid-season leaf material, collected July 25, 2001.

text: Leaf

characteristic: organ

text: Vitis vinifera

text: SfiI

text: SfiI

text: Hermaphrodite

characteristic: sex

text: Cabernet Sauvignon Leaf - CA23EI

text: Plasmid

text: pDNR


text: UC Davis, Plant Pathology

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Cabernet Sauvignon

text: CA32EN is a cDNA library of Cabernet Sauvignon leaves. The leaves were collected on July 25, 2001, in Napa Valley, California, and represent leaves in mid-season development. These leaves were asymptomatic and verified to be non-infected with the bacterial pathogen, Xylella fastidiosa, based on a diagnostic assay using PCR and Xylella-specific primer pairs. cDNA made by oligo-dT priming and directionally cloned. 5'and 3' adaptors were used in cloning as follows: 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' and 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)NN-3'. Library was constructed using the Clontech Creator SMART kit and size-selected to contain the 0.5-3 kb size fraction.

text: Leaf

text: Vitis vinifera

text: SfiI

text: SfiI

text: Hermaphrodite

text: Cabernet Sauvignon Leaf - CA32EN

text: Plasmid

text: pDNR


text: UC Davis, Plant Pathology

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Cabernet Sauvignon

text: CA48EN is a cDNA library of Cabernet Sauvignon leaves. The leaves were collected on July 25, 2001, in Napa Valley, California, and represent leaves in mid-season development. These leaves were asymptomatic and verified to be non-infected with the bacterial pathogen, Xylella fastidiosa, based on a diagnostic assay using PCR and Xylella-specific primer pairs. cDNA made by oligo-dT priming and directionally cloned. 5'and 3' adaptors were used in cloning as follows: 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' and 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)NN-3'. Library was constructed using the Clontech Creator SMART kit and size-selected to contain the 0.5-3 kb size fraction.

text: Berry stage I

text: Berry

text: Vitis vinifera

text: SfiI

text: SfiI

text: Hermaphrodite

text: Cabernet Sauvignon Leaf-CA48EN

text: Plasmid

text: pDNR


text: UC Davis, Plant Pathology

text: live

text: Generic

characteristic: NCBI submission package

text: Cabernet Sauvignon

text: CAB2SG is a cDNA library of Cabernet Sauvignon Clone 8 berries. Samples were collected at veraison (transition between stage II and stage III of berry growth) from field-grown vines 60 days after full bloom. Berries were still green but soft. Sampled vines were located at the University of California, Davis, experimental vineyard. cDNAs were made by oligo-dT priming and directionally cloned. 5'and 3' adaptors were used in cloning as follows: 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' and 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)NN-3'. Library was constructed using the Clontech Creator SMART kit and size-selected to contain the 0.5-3 kb size fraction.

text: Veraison

characteristic: dev-stage

text: Berry

text: Vitis vinifera

text: SfiI

text: SfiI

text: Hermaphrodite

text: Cabernet Sauvignon Berry - CAB2SG

text: Plasmid

text: pDNR


text: Chulalongkorn University

text: live

text: Generic

text: The mRNA was isolated from hypopharyngeal glands of Apis cerana and converted to cDNA by A superscript plasmid system for cDNA synthesis and plasmind cloning kit. The cDNA was directionally cloned into pSPORT 1 at Not I and Sal I cloning sites.

text: nurse bee

text: E. coli DH5

text: Apis cerana

characteristic: organism

text: Not I

text: Sal I

text: female

text: hypopharyngeal gland

text: Hypopharyngeal glands cDNA library

text: plasmid

text: pSPORT 1


text: College of Life Sciences, Peking University

text: live

text: Generic

text: The library was constructed using PCR-select cDNA subtraction method with 10 dpa cotton fiber as tester and fiberless mutant as driver

text: 10 days post anthesis (dpa)

text: Gossypium hirsutum

text: fiber

text: Cotton fiber subtracted cDNA library

characteristic: title


text: UMR 5555 CNRS - Univ. Perpignan

text: live

text: Generic

characteristic: cell-line

text: Differential display. Biomphalaria glabrata embryonic (Bge) cells were exposed to a) CBSS, b) CBSS containing excretory-secretory products of larval Schistosoma mansoni or c) Echinostoma caproni. Total RNA was isolated from Bge cells and submitted to a differential display analysis according to standart procedure (RNA image kit, GenHunter Corp.). Differentially displayed fragments were cloned (TOPO TA Cloning kit, Invitrogen) and sequenced.

characteristic: description

text: Biomphalaria glabrata

text: EcoRI

text: Bge cells differential display

text: pCR 2.1-TOPO


text: Genome Institute of Singapore

text: live

text: Generic

text: Priming method: Sfi-(dT)30 Primed ; Priming sequence: 5.ATTCTAGA GGCCGAGGCGGCC GACATG(T)30VN ; Directionally cloned, 5' cloning site: Sfi A site GGCCATTACGGCC ; 5' linker/adaptor sequence: 5.AAGCAGTGGTATCAACGCAGAGTGGCC ; 3' cloning site: Sfi B site GGCCGCCTCGGCC ; 3' linker/adaptor sequence: same as the priming sequence ; Average insert size: 2kb ; For PCR insert analysis: Use M13 Forward and reverse primers ; Library Amplified Recombinants (inserts): 98% ; Library complexity: 5x106 ; Full-length construction (method): SMART, a Clontech method ; Library constructed by: S. Mathavan, Chia-Lin Wei, and Yijun Ruan Genome Institute of Singapore

text: 7 Different embryonic Stages( From just fertilized Embryos to 72 hours just hatched baby fish)

characteristic: lab-host

text: Danio rerio

text: Sfi A (GGCCATTACGGCC)

text: Sfi B (GGCCGCCTCGGCC)

text: Embryo

characteristic: tissue-type

text: Plasmid

text: pDNR-LIB


characteristic: External Id

text: Case Western Reserve University

text: live

text: Generic

text: 12 h, 18h and 24 h after in vitro culture. Purified by gradient centrifugation. To construct the ookinete library, blood-stage parasite cDNAs were subtracted from a pool of ookinete sequences of 3 different developmental stages (12, 18 and 24 h)

text: Plasmodium berghei

text: ookinete library

text: pGEM-T-Easy

characteristic: vector


text: Washington University School of Medicine

characteristic: INSDC center name

text: live

text: Generic

text: cDNA was reverse transcribed using Superscript II and an anchored (?wobble') oligo dT primer, containing a NOTI restriction site, according to standard protocols. Blunt second strand cDNA was digested with NOTI restriction endonuclease, purified and size fractionated with a SizeSep 400 spun column (Amersham) and directionally cloned into the SmaI and NotI sites of pBluescript SK. Following electroporation into ElectronTen Blue cells (Stratagene), 5 x 106 primary CFU were bottle amplified in semisolid culture media [2xLB+15% SeaPrep agarosed (BMA)]. After 48 hrs at 30 degrees Celcius, cells were harvested, pooled and stored in 2xLB + 15% glycerol .

text: 3 day

text: ElectroTen Blue cells

text: Toxoplasma gondii

text: NotI

text: SmaI

text: TgME49 3 day invitro bradyzoite

text: plasmid

text: pBluescript SK


text: INRA

text: live

text: Generic

text: Compression wood was induced in four 14-year old maritime pine clones by artificial bending. Control clone (no bending) were sampled for xylem associated with early wood and late wood at the beginning (April) and at the end (August) of the growing season. Differentiating xylem corresponding to early wood, late wood, compression wood and opposite wood were used for mRNA fingerprinting using the cDNA-AFLP assay

text: Pinus pinaster

text: xylem

text: Pinus pinaster induced compression wood


text: The Sanger Centre

text: live

text: Generic

text: Homo sapiens

text: Homo sapiens cDNA fragment isolated by PCR


text: Purdue University

text: live

text: Generic

text: The cowpea weevil cDNA library was constructed containing the four larva stages and it was enriched with gut-expressing genes.

text: Larvae

text: Callosobruchus maculatus

text: Cowpea weevil larvae Lambda Zap Express Library


text: Institute of Tropical Medicine

text: live

text: Generic

text: hemocytes

characteristic: cell type

text: Insert was amplified using M13f and M13r vector specific primer. Total RNA was isolated from hemocytes of unexposed Biomphalaria glabrata (13-16-R1) snails. 5'-RACE-PCR was carried out using a gene specific primer (sequence see above) and the SMART RACE cDNA Amplification Kit (Clontech,PaloAlto,CA). The PCR-product was cloned into the plasmid vector pCR4-TOPO (Invitrogen,Groningen,Netherlands) and then subjected to sequencing

text: adult

text: E.coli

text: Biomphalaria glabrata

text: hermaphrodite

characteristic: strain

text: Biomphalaria glabrata 13-16-R1 hemocytes M13

text: plasmid


text: Radboud University Nijmegen Medical Centre

characteristic: INSDC first public

text: live

text: Generic

text: Subtraction of cochlea cDNA (tester) was performed with the PCR-Select cDNA Subtraction Kit (Clontech). Driver cDNA consisted of a mixture of cDNAs from brain, kidney, liver, heart, skeletal muscle, placenta, stomach (mucosal layer) and psoas muscle. Tester and driver cDNAs were digested by RsaI; adaptors were ligated to the tester. Two rounds of subtractive hybridization were performed, followed by two suppression PCR reactions. Subtracted PCR products were ligated into a T/A vector (Invitrogen).

text: 16-22 week fetus

text: ear

characteristic: organ

text: Homo sapiens

text: cochlea

text: subtracted cochlea cDNA library

text: pCRII


text: The University of Liverpool

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: RT-PCR of tissue RNA, gel purification of bands, clone into pGEM-T

text: E.coli JM109

text: Homo sapiens

text: overhanging T cloning of PCR pro

text: cts

text: tumor esophagus, skin, fetal brain

text: RT-PCR products from various human tissue

text: plasmid

text: pGEM-T


text: University of Nevada, Reno

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: As per Stratagene's pBluescript II XR library construction kit

text: adult

text: Ips pini

text: male

characteristic: sex

text: midgut

text: JH III-treated male I. pini midguts

text: Phagemid

text: Lambda Uni-Zap XR, Bluescript SK-


text: Sino-Germany Joint Research institute, Nanchang University

text: live

text: Generic

text: Total RNA was isolated from mycelium of Monascus aurantiacus cultured 14 days after inoculation with spores. cDNA was prepared and used as tester in suppression subtractive hybridization (Clontech). The SSH-cDNA fragments were ligated into the pMD-18T vector(Takara). Plasmid containing cDNA inserts were propagated in E.coli JM109 cells(Promega).

text: 14 days after inoculation with Monascus aurantiacus spores

characteristic: dev-stage

text: Monascus aurantiacus

characteristic: organism

text: M.aurantiacus subtracted library

characteristic: title


text: live

text: Generic

text: cDNA primed using oligo-dT primer, directionally cloned into UDG sites of pAMP1. Size selected for insert sizes ranging from 0.2-1.8 kb. Normalized to Cot5. Primary library, non-amplified. Library constructed by M. Lovett. For more information on this library, please contact R. Tidwell (Washington University) or visit the COGENE website at http://hg.wustl.edu/COGENE/.

characteristic: description

text: 6 weeks postconception

characteristic: lab-host

text: Homo sapiens

text: maxilla, pooled

text: COGENE 6E MAX

text: plasmid (ampicillin resistant)


characteristic: External Id

text: Union Hospital of Fujian Medical University

text: live

text: Generic

text: leukemia cells

text: Adult

text: Homo sapiens

text: EcoR 1

text: EcoR 1

text: 2002-SS H

text: pT-adv


text: Institute of Oceanology,Chinese Academy of Science(IOCAS)

text: live

text: Generic

text: Using Stratagene's Uni-ZAP XR cDNA synthesis Kit, we constructed a cDNA library of Bay scallop. 4975 ESTs were gained.

text: maturation phage

text: whole body

text: Argopecten irradians

text: XhoI

text: EcoRI

text: hermaphrodite

text: whole body

characteristic: tissue-type

text: Bay scallop Uni-ZAP XR Expression Library

text: Phagemid

text: pBluescript(+/-)

characteristic: vector


text: University of Florida, IFAS

characteristic: INSDC center name

text: live

text: Generic

text: Valencia

characteristic: cultivar

text: 6 hr after application of CMNP abscission agent

text: Citrus sinensis

text: peel (flavedo)

text: Mature peel (flavedo) cDNA subtraction library


text: USDA, ARS, US Meat Animal Research Center

characteristic: INSDC first public

text: live

text: Generic

text: Library made with RNA pooled from multiple tissues including liver, lung, hypothalamus, pituitary, and placenta/endometrium.

text: Bos taurus

text: EcoRI

text: NotI

text: pooled

text: MARC 6BOV

text: plasmid


text: live

text: Generic

text: Library made with RNA pooled from multiple tissues including ovary, hindbrain, uterus, and day-30 whole embryos.

text: Bos taurus

text: EcoRI

text: NotI

text: pooled

text: MARC 7BOV

text: plasmid


text: live

text: Generic

text: Library made with RNA pooled from multiple tissues including brain, liver, muscle, placenta/endometrium, ovary, testes, and bone marrow.

text: Sus scrofa

text: EcoRI

text: NotI

text: pooled

text: MARC 3PIG

text: plasmid


text: live

text: Generic

text: Library made with combined RNA from day-10, day-13, day-15, day-25, and day-30 whole embryos.

text: Sus scrofa

text: EcoRI

text: NotI

text: pooled

text: MARC 4PIG

text: plasmid


text: US Department of Agriculture, Agricultural Research Service

text: live

text: Generic

text: macrophage

characteristic: cell type

text: Library made from pools of polyA selected RNA, isolated at different times post-infection (0 to 16 hrs) from African swine fever virus (isolate Pretorisuskop/96/4) infected swine macrophages. Macrophages were derived from peripheral blood mononuclear cells cultured for 48 hrs on plastic in the presence of 30% L929 supernatant.

text: Sus scrofa

text: NotI

text: SalI

text: lymphoid

text: cd


characteristic: INSDC last update

text: live

text: Generic

text: macrophage

text: Sus scrofa

text: NotI

text: SalI

text: lymphoid

text: sn


text: live

characteristic: INSDC status

text: Generic

text: macrophage

text: Library made from pools of polyA selected RNA. Macrophages were derived from peripheral blood mononuclear cells cultured for 48 hrs on plastic in the presence of 30% L929 supernatant.

text: Sus scrofa

text: NotI

text: SalI

text: lymphoid

text: jns


text: Institute of Plant Science

text: live

text: Generic

characteristic: NCBI submission model

text: Four-day seedlings

text: Oryza sativa

text: roots

text: Aluminum induced fragments in rice roots

text: PUCm-T


text: University of Victoria

text: live

text: Generic

characteristic: NCBI submission package

text: Library Creator: Kristian R von Schalburg ; Rainbow trout tissue contributors: Mountain Trout Sales (Sooke, B.C.)

text: Oncorhynchus mykiss

text: Karl Vierke

characteristic: strain

text: Oncorhynchus mykiss reproductive


text: University of Victoria

text: live

text: Generic

text: Library Creator: Kristian R von Schalburg ; Lake whitefish tissue contributor: Louis Bernatchez (Universite Laval)

text: Coregonus clupeaformis

text: Coregonus clupeaformis head

text: pBlueScriptIISK+


text: University of Victoria

text: live

text: Generic

text: Library Creator: Kristian R von Schalburg ; Rainbow smelt tissue contributor: Vanya Ewart (NRC Institute for Marine Biociences, Halifax, N.S.)

text: Osmerus mordax

text: Osmerus mordax liver

text: pBlueScriptIISK+


text: University of Victoria

text: live

text: Generic

text: Library Creator: Kristian R von Schalburg ; Chinook salmon tissue contributor: Robert Devlin (DFO, Vancouver, B.C.)

text: Oncorhynchus tshawytscha

text: Oncorhynchus tshawytscha mixed_tissue

text: pBlueScriptIISK+


text: University of Victoria

text: live

text: Generic

text: Oncorhynchus mykiss

text: Karl Vierke

text: Oncorhynchus mykiss head

text: pBlueScriptIISK+


text: University of Victoria

text: live

text: Generic

text: Library Creator: Matthew L Rise, BF Koop ; Rainbow trout tissue contributors: Vancouver Island Trout Hatchery (Duncan B.C.)

text: Oncorhynchus mykiss

text: Tzenaicut Lake

text: Oncorhynchus mykiss Tzenaicut Lake whole

text: pBlueScriptIISK+


text: University of Victoria

text: live

text: Generic

text: Library Creator: Matthew L Rise, BF Koop ; Rainbow trout tissue contributors: Robert Devlin (DFO, Vancouver, B.C.)

text: Oncorhynchus mykiss

text: Chilliwack River steelhead

text: Oncorhynchus mykiss Chilliwack River steelhead whole

text: pBlueScriptIISK+


text: Institution of Pharmacology Research

text: live

text: Generic

text: beta-cell

text: NIT-1 cell

characteristic: cell-line

text: using RD-PCR technique to isolate the differential expressed genes.

characteristic: dev-stage

text: Pancreatic

characteristic: organ

text: Mus musculus

text: XbaI

text: SalI

text: female

characteristic: sex

text: NOD/Lt

text: islet

text: DNA fragments from the beta-cell line-NIT-1 cell

text: Plasmid

text: PMD18-T vector


text: Agricultural and Animal science University of Changchun

text: live

text: Generic

text: Leaf

text: Hordeum brevisubulatum

characteristic: organism

text: Hordeum brevisubulatum (Trin.)Link leaf cDNA

characteristic: title


characteristic: External Id

text: Southwest Missouri State University-Mountain Grove

text: live

text: Generic

text: Paulsen 1103

text: VCXR-Einhellig-1 is a cDNA library from young root tissues of grapevine rootstock Paulsen 1103(a hybrid of Vitis cinerea X V.rupestris). Paulsen 1103 grapevines were grown under normal greenhouse conditions. Roots were not subject to any stress treatments. The cDNA synthesis and library construction was performed following the guidelines provided with pBluescript II XR cDNA library constuction kit provided by stratagene.

characteristic: description

text: XL10-Gold E.coli

characteristic: lab-host

text: Vitis cinerea x Vitis rupestris

text: Xho I

text: EcoR I

text: rootstock; young root

characteristic: tissue-type

text: pBluescript II SK(+) Phagemid


text: University of Arizona

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: Gene specific primers used to amplify gene fragments from 1st strand cDNA

text: 7 to 10 day old

text: Arabidopsis thaliana

text: Ecotype Columbia

text: Seedlings

text: Cloned RT-PCR products of Arabidopsis thaliana


characteristic: INSDC last update

text: live

text: Generic

text: The library was constructed according Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. Denatured RNA was size fractionated on a 1% agarose gel. First strand cDNA synthesis was primed with oligo-dT primer containing a Not I site. Double strand cDNA was size selected according to mRNA size fraction, ligated with EcoR I adaptor, digested with NotI and then cloned directionally into pYX-Asc vector. The library tag sequence located between the Not I site and the polyA tail is CGAACTGAAT. This library was created for the University Iowa Brain Anatomy Project (BMAP): 'Gene Discovery in the Developing Mouse Nervous System', supported by National Institute of Mental Health (NIMH), Hemin Chin, Ph.D., program coordinator.

text: 1, 5, and 15 days newborn

text: DH10B (T1 phage resistant)

text: Brain

text: Mus musculus

text: EcoR I

text: Not I

text: Whole brain

text: Plasmid

text: pYX- Asc

characteristic: vector


text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: The library was constructed according Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. Denatured RNA was size fractionated on a 1% agarose gel.First strand cDNA synthesis was primed with oligo-dT primer containing a Not I site .Double strand cDNA was size selected according to mRNA size fraction, ligated with EcoR I adaptor, digested with NotI and then cloned directionally into pYX-Asc vector . The library tag sequence located between the Not I site and the polyA tail is AGCGAGACAG. This library was created for the University Iowa Brain Anatomy Project (BMAP): 'Gene Discovery in the Developing Mouse Nervous System', supported by National Institute of Mental Health (NIMH), Hemin Chin, Ph.D., program coordinator.

text: embryo 13.5,14.5,16.5,17.5dpc

text: DH10B (T1 phage resistant)

text: Brain

text: Mus musculus

text: EcoR I

text: Not I

text: whole brain

text: Plasmid

text: pYX- Asc


text: National Cancer Institute / NIH

text: live

text: Generic

characteristic: NCBI submission package

text: NCI_CGAP_FT2 is a subtracted cDNA library constructed from a pool of 81 RNA samples from Alveolar Macrophages challenged with different treatments. The mRNA samples were a mixture of these conditions (times refer to incubations following isolation by bronchoalveolar lavage) (some normal donor macrophages were cultured in some of the conditions, other donor macrophages in different conditions). The mRNA samples were pooled for library construction. Control 0 hours; control 3 hours; control 24 hours; LPS 100 ng/ml, 3 hours; LPS 100 ng/ml, 24 hours; PMA 10 ng/ml, 3 hours; PMA 10 ng/ml, 24 hours; Klebsiella moi 10, 3 hours; Klebsiella moi 10, 24 hours; Staph aureus moi 10, 3 hours; Staph aureus moi 10, 24 hours; Adenoviral vector (Ad5 CMV eGFP), moi 500, 3 hours; Adenoviral vector (Ad5 CMV eGFP), moi 500, 24 hours; wt adenovirus moi 500, 3 hours; wt adenovirus moi 500, 24 hours; Ad vector + LPS 3 hours; Ad vector + LPS 24 hours; wt adenovirus + LPS 3 hours; wt adenovirus + LPS 24 hours. The library was subtracted according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. The tissue was provided by Dr. Gary W. Hunninghake of the University of Iowa.

text: Adult

text: DH10B (Life Technologies)

text: Lung

text: Homo sapiens

text: EcoR I

text: Not I

text: Alveolar Macrophage

text: Plasmid

text: pT7T3-Pac (Pharmacia) with a modified polylinker


text: ESALQ - University of Sao Paulo

text: live

text: Generic

text: SEL 1308

characteristic: cultivar

text: mRNA from bean tissue was extracted with the FastTrack 2.0 kit (Invitrogen) and mRNA sample quality was checked in 1.2% formaldehyde-agarose gel. cDNA was synthesized and cloned into either pCMV Sport 6 or the pSPORT 1 vector with the SuperScript Plasmid System with Gateway Technology kit (Gibco BRL) as indicated by the manufacturer. Recombinant colonies were cultured in 96-well plates and maintained in liquid LB medium with ampicillin (100ug/ml) and 8% glycerol at -80oC. Plasmid miniprep was conducted in 96-well plates using standard procedure and sequencing of the cDNA was performed in 96-well PCR plates using the Big Dye Terminator Cycle Sequencing Ready Reaction Kit (Applied Biosystems). Clones were sequenced from the 5' end using either M13 Reverse or the T7 primer. PCR file consisted of 25 cycles of 10 sec. at 96oC, 5 sec. at 50oC, and 4 min. at 60oC. After PCR product precipitation, large-scale automated sequencing was carried out in a capillary system (3700 ABI Sequencer, Applied Biosystems). Raw data from sequencing was validated in a series of automated routine analyses including Phred/CAP3/Consed programs and Perl scripts created by our team. Reads that did not have at least 300 bases with Phred quality of 20 were not included in the analysis.

text: 10 days after seed germination

text: E. coli DH10B

text: Phaseolus vulgaris

text: Not I

text: Sal I

text: shoot

text: Common bean seedling EST Library - PVEPSE2

text: plasmid

text: p SPORT 1


text: ESALQ - University of Sao Paulo

text: live

text: Generic

text: SEL 1308

text: The identity of race 73 of Colletotrichum lindemuthianum was confirmed by the reaction observed on a cultivar differential series (Melotto et al., 1999). This race was grown from monosporic cultures maintained in fungus-colonized filter papers stored at -20oC. Inoculum preparation, inoculation methods, and disease characterization were conducted as described elsewhere (Melotto and Kelly 2000). Symptoms were evaluated seven days after inoculation. Resistant (SEL 1308) and susceptible (Black Magic) cultivars presented extreme disease reactions to the chosen race. mRNA to construct the PVEPSE3 library was extracted from seedlings 65 hours after inoculation. mRNA from bean tissue was extracted with the FastTrack 2.0 kit (Invitrogen) and mRNA sample quality was checked in 1.2% formaldehyde-agarose gel. cDNA was synthesized and cloned into the pCMV SPORT 6 vector with the SuperScript Plasmid System with Gateway Technology kit (Gibco BRL) as indicated by the manufacturer. Recombinant colonies were cultured in 96-well plates and maintained in liquid LB medium with ampicillin (100ug/ml) and 8% glycerol at -80oC. Plasmid miniprep was conducted in 96-well plates using standard procedure and sequencing of the cDNA was performed in 96-well PCR plates using the Big Dye Terminator Cycle Sequencing Ready Reaction Kit (Applied Biosystems). Clones were sequenced from the 5' end using the M13 Reverse primer, and from the 3' end using the T7 primer. PCR file consisted of 25 cycles of 10 sec. at 96oC, 5 sec. at 50oC, and 4 min. at 60.C. After PCR product precipitation, large-scale automated sequencing was carried out in a capillary system (3700 ABI Sequencer, Applied Biosystems). Raw data from sequencing was validated in a series of automated routine analyses including Phred/CAP3/Consed programs and Perl scripts created by our team. Reads that did not have at least 300 bases with Phred quality of 20 were not included in the analysis.

text: 13 days after seed germination

characteristic: dev-stage

text: E. coli DH10B

text: Phaseolus vulgaris

text: Not I

text: Sal I

text: shoot

text: Common bean seedling EST Library inoculated with anthracnose - PVEPSE3

text: plasmid

text: pCMV SPORT 6


text: Amgen, Inc

text: live

text: Generic

text: Anemic Rat Kidney Rat kidney cDNA library from anemic rats.

text: Rattus norvegicus

text: Kidney

text: Anemic Rat Kidney (10400)

text: Plasmid


text: Amgen, Inc

text: live

text: Generic

text: Colon Rat 1 Fetal rat intestinal cDNA library.

text: Rattus norvegicus

text: SalI

text: NotI

text: intestine, fetal

text: Colon Rat 1 (10390)

text: Plasmid

text: pMOB


text: Amgen, Inc

text: live

text: Generic

text: Colon Rat 2 Fetal rat intestinal library

text: Rattus norvegicus

text: intestine, fetal

text: Colon Rat 2 (10398)

text: Plasmid

text: pMOB


text: Amgen, Inc

text: live

text: Generic

text: Colon rat 3 Fetal rat intestine

text: Rattus norvegicus

text: intestine, fetal

text: Colon rat 3 (10415)

text: Plasmid


text: Amgen, Inc

text: live

text: Generic

text: Normal rat hypothalamus cDNA

text: Rattus norvegicus

text: XbaI

text: NotI

text: hypothalamus normal

text: PLAP-a hypothalamus (10614)

text: Plasmid


text: Amgen, Inc

text: live

text: Generic

text: Normal rat hypothalamus cDNA

text: Rattus norvegicus

text: XbaI

text: NotI

text: hypothalamus

text: PLAP-b hypothalamus (10615)

text: Plasmid


text: Amgen, Inc

text: live

text: Generic

text: Normal hypothalamus

text: Rattus norvegicus

text: XbaI

text: NotI

text: hypothalamus normal

text: PLAP-c hypothalamus (10616)

text: Plasmid


text: Amgen, Inc

text: live

text: Generic

text: artificial construct

characteristic: cell type

text: artificial construct for rat sequencing requests

text: Rattus norvegicus

text: RDS RAT (13984)


text: Amgen, Inc

text: live

text: Generic

text: artificial construct

text: artificial construct for unknown sequencing requests

text: Rattus norvegicus

text: RDS UNKNOWN (13985)


text: Amgen, Inc

text: live

text: Generic

text: Hypothalamus cDNA

text: Rattus norvegicus

text: HindIII

text: NotI

text: hypothalamus

text: Rat Hypothal cDNA (10528)

text: Plasmid


text: Amgen, Inc

text: live

text: Generic

text: SD rat hypothalamus

text: Rattus norvegicus

text: hypothalamus

text: SD rat hypothalamus (10460)

text: Plasmid


text: Amgen, Inc

text: live

text: Generic

text: W Rat hypo,frac 10 fraction 10

text: Rattus norvegicus

text: hypo,frac

text: W Rat hypo,frac 10 (10482)

text: Plasmid


text: Amgen, Inc

text: live

text: Generic

text: W Rat hypothalamus adult female Wistar rat avg. insert size 2.3 kb fraction 6 and 7

text: Rattus norvegicus

text: W Rat hypothalamus (10464)

text: Plasmid


text: Amgen, Inc

text: live

text: Generic

text: Rattus norvegicus

text: W Rat hypothalamus (10470)

text: Plasmid


text: Amgen, Inc

text: live

text: Generic

text: Rattus norvegicus

text: W Rat hypothalamus (10471)

text: Plasmid


text: Amgen, Inc

text: live

text: Generic

text: Rattus norvegicus

text: hypothalamus

text: W Rat hypothalamus (10480)

text: Plasmid


text: Amgen, Inc

text: live

text: Generic

text: W Rat hypothalamus

text: Rattus norvegicus

text: hypothalamus

text: W Rat hypothalamus (10735)

text: Plasmid


text: Amgen, Inc

text: live

text: Generic

text: W Rat pituitary adult female Wistar rat avg insert size 2.1 kb

text: Rattus norvegicus

text: W Rat pituitary (10472)

text: Plasmid


text: Amgen, Inc

text: live

text: Generic

text: Rattus norvegicus

text: pituitary

text: W Rat pituitary (10477)

text: Plasmid


text: Amgen, Inc

text: live

text: Generic

text: Rattus norvegicus

text: pituitary

text: W Rat pituitary (10481)

text: Plasmid


text: Amgen, Inc

text: live

text: Generic

text: W rat hypo+pit Wistar rat

text: Rattus norvegicus

text: hypo+pit

text: W rat hypo+pit (10478)

text: Plasmid


text: Amgen, Inc

text: live

text: Generic

text: W white adipose tiss adult female Wistar rats, >250 grams

text: Rattus norvegicus

text: adipose tiss

text: W white adipose tiss (10479)

text: Plasmid


text: Amgen, Inc

text: live

text: Generic

text: rat brain motor neuron

text: Rattus norvegicus

text: brain motor neuron

text: brain motor neuron (10217)

text: Plasmid


text: Amgen, Inc

text: live

text: Generic

text: Chung Model Rat DRG Left L5/L6

text: Rattus norvegicus

text: Chung Model Ipsilate

text: cdrg1 (10898)

text: Plasmid


text: Amgen, Inc

text: live

text: Generic

text: peptides and housekeeping

text: Rattus norvegicus

text: Peptide and housekee

text: cdrg2 (10902)

text: Plasmid

text: multiple


text: Amgen, Inc

text: live

text: Generic

text: prostate NBE 1.4

characteristic: cell-line

text: NBE 1.4 cell line, rat normal prostate.

text: Rattus norvegicus

text: hrnp1 (10408)

text: Plasmid


text: Amgen, Inc

text: live

text: Generic

text: prostate NBE 1.4

text: NBE 1.4, rat normal prostrate, subtracted with rat liver

text: Rattus norvegicus

characteristic: organism

text: hrnp3 (10419)

text: Plasmid

text: pCRII


text: Amgen, Inc

text: live

text: Generic

text: kidney embryo D18 rat kidney embryo day 18

text: Rattus norvegicus

text: kidney embryo

text: kidney embryo D18 (10815)

characteristic: title

text: Plasmid


characteristic: External Id

text: Amgen, Inc

text: live

text: Generic

text: rat brain motor neuron subrtacted and rearrayed from mrmn1

text: Rattus norvegicus

text: brain motor neuron

text: mrmn3 (10218)

text: Plasmid


text: Amgen, Inc

text: live

text: Generic

text: placenta embryo day 17

characteristic: description

text: Rattus norvegicus

text: NotI

text: placenta embryo

text: mrpe4 (10380)

text: Plasmid


text: Amgen, Inc

text: live

text: Generic

text: placenta embryo D17

text: Rattus norvegicus

text: placenta embryo D17

text: mrpf1 (10740)

text: Plasmid


text: Amgen, Inc

text: live

text: Generic

text: rat dorsal root ganglia

text: Rattus norvegicus

text: Dorsal Root Ganglia

text: nrdg1 (10855)

text: Plasmid


text: Amgen, Inc

text: live

text: Generic

text: dorsal root ganglia

text: Rattus norvegicus

text: dorsal root ganglia

text: nrdr1 (10722)

text: Plasmid

text: pCMVI


text: Amgen, Inc

text: live

text: Generic

text: Rattus norvegicus

text: nrhy7 (10850)

text: Plasmid


text: Amgen, Inc

text: live

text: Generic

text: hypothalamus

text: Rattus norvegicus

text: hypothalamus

text: nrhz1 (10741)

text: Plasmid


text: Amgen, Inc

text: live

text: Generic

text: pheochromocytoma adrenal cells

text: Rattus norvegicus

text: NotI

text: adrenal

text: nrpc1 (10212)

text: Plasmid

text: pX


text: Amgen, Inc

text: live

text: Generic

text: membraneous labyrinth of ear; associated with balance

text: Rattus norvegicus

text: NotI

text: labyrinth of ear

text: nrut1 (10213)

text: Plasmid

text: pX


text: Amgen, Inc

text: live

text: Generic

text: white adipose tiss adult female Wistar rats, >250 grams

text: Rattus norvegicus

text: white adipose tiss

text: nrwb1 (10739)

text: Plasmid


text: Amgen, Inc

text: live

text: Generic

text: rat anterior pituitary

text: Rattus norvegicus

text: HindIII

text: pituitary

text: pituitary (10543)

text: Plasmid


text: Amgen, Inc

text: live

text: Generic

text: rat anterior pituitary

text: Rattus norvegicus

text: HindIII

text: pituitary; subtracte

text: pituitary (10545)

text: Plasmid


text: Amgen, Inc

text: live

text: Generic

text: placenta embryo D17

text: Rattus norvegicus

text: placenta embryo

text: placenta embryo D17 (10377)

text: Plasmid


text: Amgen, Inc

text: live

text: Generic

text: placenta embryo D17

text: Rattus norvegicus

text: placenta embryo

text: placenta embryo D17 (10379)

text: Plasmid


text: Amgen, Inc

text: live

text: Generic

text: rat brain E15

text: Rattus norvegicus

text: BstXI

text: brain E15

text: rat brain E15 (10373)

text: Plasmid

text: pBCB


text: Amgen, Inc

text: live

text: Generic

text: rat brain E15

text: Rattus norvegicus

text: BstXI

text: brain E15

text: rat brain E15 (10374)

text: Plasmid

text: pBCB


text: Amgen, Inc

text: live

text: Generic

text: rat brain E15

text: Rattus norvegicus

text: BstXI

text: brain E15

text: rat brain E15 (10375)

text: Plasmid

text: pBCB


text: Amgen, Inc

text: live

text: Generic

text: rat hypothalamus size selected (300-1000bp)

text: Rattus norvegicus

text: XhoI

text: NotI

text: hypothalamus

text: rat hypothalamus (10182)

text: Plasmid


text: Amgen, Inc

text: live

text: Generic

text: hypothalamus

text: Rattus norvegicus

text: HindIII

text: hypothalamus

text: rat hypothalamus (10527)

text: Plasmid


text: Amgen, Inc

text: live

text: Generic

text: choroid plexus brain region

text: Rattus norvegicus

text: NotI

text: choroid plexus brain

text: srcp1 (10190)

text: Plasmid


text: Amgen, Inc

text: live

text: Generic

text: choroid plexus brain region

text: Rattus norvegicus

text: NotI

text: choroid plexus brain

text: srcp2 (10233)

text: Plasmid


text: Amgen, Inc

text: live

text: Generic

text: rat corneas

text: Rattus norvegicus

text: EcoRI

text: EcoRI

text: Cornea

text: srcs1 (10883)

text: Plasmid


text: Amgen, Inc

text: live

text: Generic

text: islet cells

text: islet cells

text: Rattus norvegicus

text: sric1 (10763)

text: Plasmid

text: pBluescript II SK(+)


text: Amgen, Inc

text: live

text: Generic

text: rat prostate normalized double selected poly(A+) mRNA

text: Rattus norvegicus

text: NotI

text: prostate tissue

text: srpa1 (10191)

text: Plasmid


text: Amgen, Inc

text: live

text: Generic

text: rat prostate normalized double selected poly(A+) mRNA size fraction > 1 kb

text: Rattus norvegicus

text: NotI

text: prostate tissue

text: srpb1 (10313)

text: Plasmid


text: Amgen, Inc

text: live

text: Generic

text: Rattus norvegicus

text: NotI

text: prostate tissue

text: srpb2 (10220)

text: Plasmid


text: Amgen, Inc

text: live

text: Generic

text: peneal gland brain region

text: Rattus norvegicus

text: NotI

text: peneal gland brain r

text: srpg1 (10201)

text: Plasmid


text: Amgen, Inc

text: live

text: Generic

text: peneal gland brain region

text: Rattus norvegicus

text: NotI

text: peneal gland brain

text: srpg2 (10238)

text: Plasmid


text: Amgen, Inc

text: live

text: Generic

text: thyroid and parathyroid

text: Rattus norvegicus

text: thyroid and parathyr

text: srth1 (10343)

text: Plasmid


text: Amgen, Inc

text: live

text: Generic

text: rat corneas

text: Rattus norvegicus

text: EcoRI

text: cornea

text: srvc1 (10901)

text: Plasmid


text: Amgen, Inc

text: live

text: Generic

text: adipose tissue preselected prior to signal trap

text: Rattus norvegicus

text: NotI

text: adipose

text: tras1 (10312)

text: Plasmid


text: Amgen, Inc

text: live

text: Generic

text: brown adipose tissue

text: Rattus norvegicus

text: NotI

text: brown adipose

text: trba1 (10260)

text: Plasmid


text: Amgen, Inc

text: live

text: Generic

text: brown adipose tissue

text: Rattus norvegicus

text: NotI

text: brown adipose

text: trba2 (10286)

text: Plasmid


text: Amgen, Inc

text: live

text: Generic

text: choroid plexus brain region

text: Rattus norvegicus

text: NotI

text: choroid plexus brain

text: trcp2 (10289)

text: Plasmid


text: Amgen, Inc

text: live

text: Generic

text: choroid plexus brain region

text: Rattus norvegicus

text: NotI

text: choriod plexus brain

text: trcx1 (10261)

text: Plasmid

text: pcAv


text: Amgen, Inc

text: live

text: Generic

text: Rat gastricnemius and soleus muscle

text: Rattus norvegicus

text: NotI

text: muscle

text: trgs1 (10605)

text: Plasmid


text: Amgen, Inc

text: live

text: Generic

text: Rat gastricnemius and soleus muscle

text: Rattus norvegicus

text: muscle

text: trgs2 (10306)

text: Plasmid


text: Amgen, Inc

text: live

text: Generic

text: islet cells

text: islet cells

text: Rattus norvegicus

text: tric1 (10762)

text: Plasmid

text: pBluescript II SK(+)


text: Amgen, Inc

text: live

text: Generic

text: anterior pituitary

text: Rattus norvegicus

text: NotI

text: anterior pituitary

text: trpa2 (10598)

text: Plasmid


text: Amgen, Inc

text: live

text: Generic

text: Rat anterior pituitary adrenal, ovary and thyroid removed approx. 21 days prior to tissue isolation

text: Rattus norvegicus

text: NotI

text: anterior pituitary

text: trpa3 (10298)

text: Plasmid


text: Amgen, Inc

text: live

text: Generic

text: peneal gland brain region

text: Rattus norvegicus

text: NotI

text: peneal gland brain

text: trpg1 (10235)

text: Plasmid


text: Amgen, Inc

text: live

text: Generic

text: peneal gland brain region

text: Rattus norvegicus

text: NotI

text: peneal gland brain

text: trpg2 (10294)

text: Plasmid


text: Amgen, Inc

text: live

text: Generic

text: rat prostate

text: Rattus norvegicus

text: HindIII

text: NotI

text: prostate

text: trxp1 (10556)

text: Plasmid


text: Amgen, Inc

text: live

text: Generic

text: rat prostate

text: Rattus norvegicus

text: HindIII

text: prostate

text: tryp1 (10582)

text: Plasmid


text: Amgen, Inc

text: live

text: Generic

text: Rat GPCR library rearrayed internal pSPORT vector

text: Rattus norvegicus

text: urgp1 (14349)


text: Amgen, Inc

text: live

text: Generic

text: Rattus norvegicus

text: urrg1 (14046)


text: Amgen, Inc

text: live

text: Generic

text: Rat toxicology library Rearrayed from internal pSPORT vector

text: Rattus norvegicus

text: urtg1 (13981)


text: Amgen, Inc

text: live

text: Generic

text: Rattus norvegicus

text: adipose tiss

text: white adipose tiss (10469)

text: Plasmid


text: Amgen, Inc

text: live

text: Generic

text: Extramural

text: Rattus norvegicus

text: Extramural

text: zrlet02 Extramural (10161)


text: Amgen, Inc

text: live

text: Generic

text: anterior pituitary

text: Rattus norvegicus

text: anterior pituitary

text: trnp1 (10361)

text: Plasmid

text: pBluescript II SK(+)


text: University of Washington

text: live

text: Generic

text: neonatal

text: placenta

characteristic: organ

text: Macaca mulatta

text: male

characteristic: sex

text: MMPL


text: University of Washington

characteristic: INSDC center name

text: live

text: Generic

text: monocytes

text: adult

text: spleen

text: Macaca mulatta

text: male

text: MMSP


text: Zentrum fur Nervenheilkunde, Philipps-Universitat Marburg

characteristic: INSDC first public

text: live

text: Generic

text: Homo sapiens

text: midbrain DA neurons

characteristic: tissue-type

text: human midbrain DA neurons library


characteristic: INSDC last update

text: live

text: Generic

text: Non-normalized full-length enriched library 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3'(where B = A, C, or G and N = A, C, G, or T). Average insert size 1.2kb (cDNA range 0.4-3.0 kb). 15/15 coloniescontained inserts by PCR.This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA).Corp.

text: DH10B TonA

characteristic: lab-host

text: Xenopus laevis

text: SfiI (directional)

text: SfiI (directional)

text: Normal testis

text: plasmid (chloramphenicol resistant, grown at 30C)

text: pDNR-LIB

characteristic: vector


text: National Cancer Institute / NIH

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: cDNA made by oligo-dT priming and directionally cloned. 5' and 3' adaptors were used in cloning as follows: 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' and 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)NN-3'. Full-length enriched library was constructed using the Clontech Creator SMART kit and size-selected to contain the 0.5 kb size fraction. Library created in the laboratory of M. Brownstein (NIMH, NIH). Note: this is a NIH_MGC Library.

text: DH10B (T1-phage-resistant)

text: liver

text: Mus musculus

text: SfiI (ggccattatggcc)

text: SfiI (ggccgcctcggcc)

text: pDNR-LIB


text: live

text: Generic

text: cDNA made by oligo-dT priming. Directionally cloned into EcoRI/XhoI sites using the following 5' adaptor: GGCACGAG(G). Library constructed by Genome Therapeutics Corp. Tissue contributed by Narayan K. Bhat using ZAP-cDNA synthesis kit (Stratagene).

text: DH10B TonA

text: Homo sapiens

text: EcoRI

text: XhoI

text: Blood, promyelocytic leukemia


text: National Cancer Institute / NIH

text: live

text: Generic

text: DH10B (T1-phage-resistant)

text: kidney

text: Mus musculus

text: SfiI (ggccattatggcc)

text: SfiI (ggccgcctcggcc)

text: pDNR-LIB


text: Henan Normal University

text: live

text: Generic

text: A rat regenerating liver cDNA library was constructed after partial hepatectomy eight hours by SSH(Suppression subtractive hybridization).

text: Rattus norvegicus

text: liver

text: rat regenerating liver cDNA library

text: pGEM-T


text: University of Florida

text: live

text: Generic

text: Bragg

characteristic: cultivar

text: Seeds were collected from Glycine max cv. Bragg plants grown in ambient or elevated carbon dioxide, and ambient or elevated temperature, in a 2x2 factorial arrangement of treatments. Total RNA was extracted, differential display was performed, and differentially displayed products were recovered, re-amplified, and cloned into pGEM-T.

text: Seed

text: Glycine max

text: Soybean Differential Display

text: Plasmid

text: pGEM-T


text: Amgen, Inc

text: live

text: Generic

text: endothelial and othe

characteristic: cell type

text: Rattus norvegicus

text: EcoRI

text: smvm1 (10899)

text: Plasmid


text: INRA - SCRIBE

text: live

text: Generic

text: Rainbow trout multi-tissues - normalized + 1 subtraction (tcay) ; Clone distribution : AGENAE Resource centre. Francois PIUMI, Francois.Piumi@jouy.inra.fr, INRA, CEA Radiobiologie et Etude du genome (LREG), Domaine de Vilvert, 78352, Jouy-en-Josas cedex, France, +33 (0) 1.34.65.28.02, +33 (0) 1.34.65.22.73

text: from embryos to adults

characteristic: dev-stage

text: Oncorhynchus mykiss

characteristic: organism

text: adipose tissue, blood, brain, differentiating gonads, gills, interrenal, intestine, kidney, liver, muscle, ovary, pituitary, testis

text: tcay


characteristic: External Id

text: INRA - SCRIBE

text: live

text: Generic

text: Clone distribution : AGENAE Resource centre. Francois PIUMI, Francois.Piumi@jouy.inra.fr, INRA, CEA Radiobiologie et Etude du genome (LREG), Domaine de Vilvert, 78352, Jouy-en-Josas cedex, FRANCE

text: from embryos to adults

text: Oncorhynchus mykiss

text: adipose tissue, blood, brain, differentiating gonads, gills, interrenal, intestine, kidney, liver, multi-tissues, muscle, ovary, pituitary, testis

text: AGENAE Rainbow trout multi-tissues subtracted library (control) (tcaz)

characteristic: title


text: The University of Tokyo, Graduate School of Frontier Sciences

text: live

text: Generic

text: adult

text: Pan troglodytes verus

text: female

text: cerebellum

text: female adult cerebellum, full-length enriched chimpanzee cDNA library


text: live

text: Generic

text: adult

text: Pan troglodytes verus

text: female

text: liver

text: female adult liver, full-length enriched chimpanzee cDNA library


text: live

text: Generic

text: adult

text: Pan troglodytes verus

text: female

text: cerebrum

text: female adult cerebrum, full-length enriched chimpanzee cDNA library


text: live

text: Generic

text: adult

text: Pan troglodytes verus

text: male

text: skin

text: male adult skin, full-length enriched chimpanzee cDNA library


text: National Institute of Agrobiological Sciences

text: live

text: Generic

text: a mixture of pupal stages after P1

characteristic: description

text: Bombyx mori

text: brain

text: brP-


text: live

text: Generic

text: a mixture stages from 5th instar larva day 3 to S3

text: Bombyx mori

text: brain

text: brS-


text: live

text: Generic

text: mixed stages from 5th instar larva to pupa

text: Bombyx mori

text: compound eye

text: ce--


text: Iowa State University

text: live

text: Generic

text: Bos taurus

text: Eco R1

text: Eco R1

text: embryo

text: Bos taurus Day 31-34 embryo

text: pT-Advantage


text: Amgen, Inc

text: live

text: Generic

text: Rattus norvegicus

text: HindIII

text: NotI

text: Hypothalamus

text: Rat Hypothal cDNA (10529)

text: Plasmid


text: Amgen, Inc

text: live

text: Generic

text: Rattus norvegicus

text: NotI

text: yolk sac mesoderm

text: smyn3 (10282)

text: Plasmid


text: Amgen, Inc

text: live

text: Generic

text: Rattus norvegicus

text: umab1 (10882)

text: Plasmid

text: pMOB


text: UC Davis, Department of Pomology

text: live

text: Generic

text: albedo

text: Washington navel orange

text: Mature citrus fruits were harvested from trees growing in the 'Citrus variety collection' in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected in January 2002, between 1 and 3 PM and stored at 4C. Albedo tissue was separated from the rest of the peel and used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The amplified library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes.

text: Mature fruit sample - collected January 2002

text: Peel/rind

characteristic: organ

text: Citrus sinensis

text: EcoRI

text: XbaI

text: Hermaphrodite

characteristic: sex

text: Mature Albedo

text: Plasmid


characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: Bombyx mori

text: compound eye

text: ceN-


characteristic: INSDC last update

text: live

text: Generic

text: 5th instar larva day 3

text: Bombyx mori

text: epidermis

characteristic: tissue-type


text: live

text: Generic

text: Bombyx mori

text: mixed

text: fat body


text: live

text: Generic

text: isolated from 2h post-baculovirus infection

text: Bombyx mori

text: fat body

text: fbpv


text: live

text: Generic

text: Bombyx mori

text: hemocyte


text: live

text: Generic

text: 4th molt

text: Bombyx mori

text: posterior silkgland


text: live

text: Generic

text: 5th instar larva day 3

text: Bombyx mori

text: posterior silkgland


text: live

text: Generic

text: spinning stage

text: Bombyx mori

text: testis

text: tesS


text: live

text: Generic

text: 5th instar larva

text: Bombyx mori

text: testis

text: tesV


text: Amgen, Inc

text: live

text: Generic

text: artificial construct

text: artificial construct

text: Rattus norvegicus

text: RDS HUMAN (13983)


text: Faculdade de Ciencias da Universidade de Lisboa

text: live

text: Generic

text: seven days after infection of Brassica oleracea

text: Hyaloperonospora parasitica

text: P005 and P501

characteristic: strain

text: Peronospora parasitica DD-PCR fragment


text: live

characteristic: INSDC status

text: Generic

text: CrGC3.1, Algarvia, Coracao-de-Boi

text: seven days after infection with Peronospora parasitica

text: Brassica oleracea

text: Brassica oleracea DD-PCR fragment


text: University of Oxford

text: live

text: Generic

characteristic: NCBI submission model

text: Testis

text: Homo sapiens

text: Testis Lambda Zap Express Library Stratagene


text: INRA

text: live

text: Generic

characteristic: NCBI submission package

text: country=France

text: adult

text: Pinus pinaster

text: differentiating xylem

text: Pinus pinaster differentiating xylem adult


text: INRA

text: live

text: Generic

text: country=France

text: Pinus pinaster

text: differentiating xylem

text: Pinus pinaster differentiating xylem


text: INRA

text: live

text: Generic

text: country=France

text: Pinus pinaster

text: Pinus pinaster library France


text: Universidad de Malaga, Facultad de ciencias

text: live

text: Generic

text: Corsican

characteristic: cultivar

text: adult

text: Pinus pinaster

text: differentiating xylem

text: Pinus pinaster Corsican differentiating xylem adult


text: Peking University

text: live

text: Generic

text: mesangial

characteristic: cell type

text: LOW

characteristic: cell-line

text: adult

text: Homo sapiens

text: Homo sapiens LOW cell line


text: Agriculture and Agri-food Canada

text: live

text: Generic

text: Westar

text: Germinated in soil flats and seedlings grown for 3 weeks in a Conviron E-15 cabinet set at 20oC /16 hr light (250 Em-2sec-1) and 16 oC / 8 hr dark. Fourth leaves collected at 9 am and immediately frozen.

text: 3 weeks seedling grown at room temperature

characteristic: dev-stage

text: Brassica napus

characteristic: organism

text: EcoRI

text: XhoI

text: fourth leaf

text: Bn01b_AAFC_ECORC_transgenic_Brassica_napus_overexpressing_BNCBF17_constitutively_frost_tolerant

text: Plasmid

text: Bluescript SK+/XhoI-EcoRI

characteristic: vector


characteristic: External Id

text: University of Maryland Biotechnology Institute

text: live

text: Generic

text: Cryphonectria parasitica EST derived from mixed samples of cDNA from wild-type (strain EP155) and hypovirus-infected cultures

characteristic: description

text: Cryphonectria parasitica

text: Mixed source, strain EP155 and EP155 infected with hypovirus CHV1-EP713

characteristic: title


text: Universita degli Studi dell'Insubria

characteristic: INSDC center name

text: live

text: Generic

text: adult

text: Dicentrarchus labrax

text: liver

text: cDNA from RT-PCR or differential display on Dicentrarchus labrax liver


text: Amgen, Inc

characteristic: INSDC first public

text: live

text: Generic

text: prostate NBE 1.4

text: Rattus norvegicus

text: prostate

text: hrnp2 (10657)

text: Plasmid


text: Amgen, Inc

characteristic: INSDC last update

text: live

text: Generic

text: Rattus norvegicus

text: NotI

text: hypothalamus normal

text: hypothalamus (10624)

text: Plasmid


text: Amgen, Inc

text: live

text: Generic

text: DRG

text: Rattus norvegicus

text: srdc1 (10884)

text: Plasmid


text: Amgen, Inc

text: live

text: Generic

text: Rattus norvegicus

text: NotI

text: choroid plexus brain

characteristic: tissue-type

text: trcp1 (10232)

text: Plasmid


text: Amgen, Inc

text: live

text: Generic

text: Rattus norvegicus

text: NotI

text: Anterior Pituitary

text: trpa1 (10593)

text: Plasmid


text: Amgen, Inc

text: live

text: Generic

text: Rattus norvegicus

text: HindIII

text: NotI

text: pituitary

text: trqi1 (10576)

text: Plasmid


text: Amgen, Inc

text: live

text: Generic

text: adipose

text: Rattus norvegicus

text: tras2 (10311)

text: Plasmid


text: Amgen, Inc

text: live

text: Generic

text: DRG

text: Rattus norvegicus

text: srcd1 (10885)

text: Plasmid


text: Amgen, Inc

text: live

text: Generic

text: Rattus norvegicus

text: NotI

text: hypothalamus normal

text: hypothalamus (10623)

text: Plasmid


text: Amgen, Inc

text: live

text: Generic

text: Rattus norvegicus

text: hypothalamus normal

text: PLAP-b hypothalamus (10617)

text: Plasmid


text: US Department of Agriculture, Agricultural Research Service

text: live

text: Generic

text: Library made from polyA selected RNA, isolated from whole stage N2 ticks two weeks after swine blood meal.

characteristic: lab-host

text: Ornithodoros porcinus porcinus

text: NotI

text: SalI

text: Whole Tick

text: op


text: UC Davis Department of Plant Pathology

text: live

characteristic: INSDC status

text: Generic

text: Cabernet Sauvignon

text: CA48LN is a cDNA library of Cabernet Sauvignon leaves. The leaves were collected on September 20, 2001, in Napa Valley, California, and represent leaves in late season development. These leaves were asymptomatic and verified to be non-infected with the bacterial pathogen, Xylella fastidiosa, based on a diagnostic assay using PCR and Xylella-specific primer pairs. cDNAs were made by oligo-dT priming and directionally cloned. 5'and 3' adaptors were used in cloning as follows: 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' and 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)NN-3'. Library was constructed using the Clontech Creator SMART kit and size-selected to contain the 0.5-3 kb size fraction.

text: Late season sample

text: Leaf

characteristic: organ

text: Vitis vinifera

text: SfiI

text: SfiI

text: hermaphrodite

characteristic: sex

text: Cabernet Sauvignon Leaf - CA48LN

text: Plasmid

text: pDNR


text: UC Davis Department of Plant Pathology

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Cabernet Sauvignon

characteristic: cultivar

text: CA41LN is a cDNA library of Cabernet Sauvignon leaves. The leaves were collected on September 20, 2001, in Napa Valley, California, and represent leaves in late season development. These leaves were asymptomatic and were verified to be non-infected with the bacterial pathogen, Xylella fastidiosa, based on a diagnostic assay using PCR and Xylella-specific primer pairs.cDNA made by oligo-dT priming and directionally cloned. 5'and 3' adaptors were used in cloning as follows: 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' and 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)NN-3'. Library was constructed using the Clontech Creator SMART kit and size-selected to contain the 0.5-3 kb size fraction.

text: Late season sample

text: Leaf

text: Vitis vinifera

text: SfiI

text: SfiI

text: hermaphrodite

text: Cabernet Sauvignon Leaf - CA41LN

text: Plasmid

text: pDNR


text: University of Massachusetts

text: live

text: Generic

text: Guzmania lingulata

text: mature leaf

text: Guzmania lingulata mature leaf


text: Department of Neurology, Johns Hopkins University

text: live

text: Generic

text: neuron

characteristic: cell type

text: mRNA was purified using Trizol and poly-dT column and cDNA library was prepared from rat cortical neurons.

text: embryonic day 16 and days in vitro 14

characteristic: dev-stage

text: brain

text: Rattus norvegicus

text: male, female

text: Sprague-Dawley

characteristic: strain

text: cortex

text: Rat cortical cDNA Library

text: plasmid


characteristic: External Id

text: Henan Normal University

text: live

text: Generic

text: A rat regenerating liver cDNA library was constructed after partial hepatectomy eight hours by SSH(Suppression subtractive hybridization). The genes are up-regulative in liver regeneration.

text: Rattus norvegicus

characteristic: organism

text: Sprague-Dawley

text: liver

text: rat regenerating liver after partial hepatectomy

text: pGEM-T

characteristic: vector


text: Department of Genetics, The Hospital for Sick Children

characteristic: INSDC center name

text: live

text: Generic

text: Homo sapiens

text: NotI

text: SalI

text: Image cDNA Library pCMV-SPORT6

characteristic: title


text: USDA - ARS

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Toxoptera citricida (Kirkaldy); A high quality EST with at least 100 contiguous bases at Trace Tuner score of 20 or better.

characteristic: description

text: Adult Alate

text: Aphis citricidus

text: EcoRI

text: XhoI

text: Mixed population

text: Entire insect

text: Adult Alate Brown Citrus Aphid

text: pBluescript II SK+


text: Martin Luther University Halle-Wittenberg, Medical Faculty

text: live

characteristic: INSDC status

text: Generic

text: adult

text: Oryctolagus cuniculus

text: hybrid strain Zika

text: rabbit liver mRNA to cDNA


text: live

text: Generic

text: adult

text: Oryctolagus cuniculus

text: hybrid strain Zika

text: rabbit skeleton muscle mRNA to cDNA


text: University of Oklahoma

text: live

text: Generic

text: sperm cell

text: Sua and Svn sperm cells were isolated and collected in separate pools using a microinjector. cDNA libraries were constructed by oligo dT priming using the Clontech SMART cDNA Library Construction kit. The cDNAs were directionally cloned into vector TriplEx2.

text: post-anthesis pollen

text: E.coli strain XL1-Blue

characteristic: lab-host

text: Plumbago zeylanica

text: Plumbago zeylanica sperm cell (Sua) cDNA library

text: Clontech TriplEx2


text: University of Oklahoma

text: live

text: Generic

text: sperm cell

text: post-anthesis pollen

text: E.coli strain XL1-Blue

text: Plumbago zeylanica

text: Plumbago zeylanica sperm cell (Svn) cDNA library

text: Clontech TriplEx2


text: Institut National de la Recherche Agronomique

text: live

text: Generic

text: Bergeron

text: Oriented library, construction described in Molecular cloning and expression of a cDNA encoding 1-aminocyclopropane-1-carboxylate (ACC) oxidase from apricot fruit (Prunus armeniaca cv. Bergeron) by Mbeguie-Mbeguie D, Chahine H, Gomez RM, Gouble B, Audergon JM, Souty M, Albagnac G, Fils-Lycaon B in Physiol Plant 105:294-303 1999

text: Half-Ripe stage

text: Fruit

characteristic: organ

text: Prunus armeniaca

text: Eco RI

text: XhoI

text: Half-Ripe Apricot Fruit Lambda Zap II Library

text: Phage

text: Lambda Zap II


text: live

text: Generic

characteristic: NCBI submission model

text: Bergeron

text: Green stage

text: Fruit

text: Prunus armeniaca

text: Eco RI

text: XhoI

text: Green Apricot Fruit Lambda Zap II Library

text: Phage

text: Lambda Zap II


text: live

text: Generic

characteristic: NCBI submission package

text: Bergeron

text: Ripe stage

text: Fruit

text: Prunus armeniaca

text: Eco RI

text: XhoI

text: Ripe Apricot Fruit Lambda Zap II Library

text: Phage

text: Lambda Zap II


text: University of California, Riverside

text: live

text: Generic

text: The cDNA was cloned into the SalI/NotI sites of pSPORT1

text: larvae

text: Aedes aegypti

text: SalI

text: NotI

text: Whole

characteristic: tissue-type

text: Aedes aegypti larval pSPORT Library


text: Wageningen University

text: live

text: Generic

text: mRNA extracted from preparasitc J2s of Globodera rostochiensis cloned directionally into pcDNAII plasmid vector.

text: Second stage juvenile

text: Globodera rostochiensis

text: EcoRI

text: NotI

text: Globodera rostochiensis J2 pcDNAII library

text: pcDNAII (Invitrogen)


text: Second Military Medical University

text: live

text: Generic

text: Mus musculus

text: palate shelves

text: Mus musculus palate shelves


text: Michigan State University

text: live

text: Generic

text: ESTs isolated from differential display experiment

text: Sus scrofa

text: Pig skeletal muscle cDNA


text: Michigan State University

text: live

text: Generic

text: Sus scrofa

text: Pig liver cDNA


text: Washington University School of Medicine

text: live

text: Generic

text: The library was constructed by Claire Murphy and Dr. Makedonka Dautova at Washington University, St. Louis. The cDNA was made by using Dynabead oligo-dT priming (Dynal). PCR based library using a modified protocol from the SMART PCR cDNA Synthesis Kit from Clontech. Directionally cloned into the UDG sites of pAMP1. Meloidogyne chitwoodi eggs were provided by Dr. David Bird at North Carolina State University, Raleigh, NC (david_bird@ncsu.edu).

text: egg

text: Meloidogyne chitwoodi

text: Not I

text: Sal I

text: Meloidogyne chitwoodi egg pAMP1 v1

text: plasmid: ampicillin resistance


text: live

text: Generic

text: The cDNA library was constructed by Keliang Tang, and Robert Cole at Washington University. PolyA mRNA was converted to cDNA using the template-switching PCR method (SMART cDNA, Clontech Inc.). First strand was reverse transcribed using the CDS III/3' primer and a 5' template switch primer (Smart IV primer). The product of the first strand synthesis was PCR amplified using the same primer set and the fragments were digested with SfiI. The fragments were size selected, ligated into a modified pBluescript vector (Michael White, Montana State University) containing directional SfiI sites, and transformed into XL10 E. coli cells. Vector: SfiI sites were added to the multiple cloning region of pBluescript SK+ between the BamHI/EcoRI sites. The modified polylinker has the following sequence: 5'GAATTCGGCCATTACGGCC(G)n-- insert-- GGCCGCCTCGGCCCACGGATCC3'where n=3-4 G nucleotides.

text: E.coli XL10 (Stratagene)

text: Neospora hughesi

text: Neospora EN-1 cDNA Library

text: plasmid

text: Modified pBluescript (pBS SK+)


text: College of Veterinary Medicine,Naning Agricultural University

text: live

text: Generic

text: Procambarus clarkii

text: Procambarus clarkii mRNA


text: University of Nevada

text: live

text: Generic

text: Library construction was performed according to Stratagene's recommended protocol for the Lambda UniZapXR vector and cDNA synthesis kit.

text: juvenile and adult

text: Vitis vinifera

text: EcoRI

text: XhoI

text: root

text: An expressed sequence tag database for abiotic stressed roots of Vitis vinifera var. Chardonnay

text: Phagemid

text: Lambda Uni-Zap XR, Bluescript SK-


text: Peking University Health Sciences Center

text: live

text: Generic

text: fibroblast

characteristic: cell type

characteristic: cell-line

text: Suppression subtractive hybridization was performed between low passage with high passage 2BS cells by two directions, then the production was ligated to vector pGEM-Teasy by T-A cloning.

text: embryonic

text: lung

text: Homo sapiens

text: female

characteristic: sex

text: Fibroblast 2BS Cells Suppression Subtractive Library

text: Plasmid

text: pGEM-Teasy


characteristic: External Id

text: University of University of Nebraska at Lincoln

text: live

text: Generic

text: epithelioid

text: First-strand cDNAs were amplified using pairwise combinations of 5 arbitrary primers and oligo-dT anchor primers (see Risatti et al. Animal Biotechnology 2003; 14:31-49). Differentially displayed bands were excised from the gels and re-amplified by PCR using the corresponding combination of primers (Liang and Pardee Science 1992; 257:967-971).

text: adult

characteristic: dev-stage

text: Bos taurus

text: male

text: kidney

text: Madin-Darby bovine kidney cell DD arbitrary-primed cDNA


text: live

text: Generic

text: Medicago sativa

characteristic: organism

text: EcoRI

text: MseI

text: root nodules

text: Medicago sativa cDNA-AFLP


text: Kellogg Eye Center, University of Michigan

characteristic: INSDC center name

text: live

text: Generic

text: Mus musculus

text: eye

text: MOUSE POST-NATAL DAY 2 EYE

characteristic: vector


characteristic: INSDC first public

text: live

text: Generic

text: Mus musculus

text: eye

text: MOUSE EMBRYONIC DAY 15.5 EYE

characteristic: title


characteristic: INSDC last update

text: live

text: Generic

text: Mus musculus

text: retina

text: MOUSE ADULT RETINA


text: live

characteristic: INSDC status

text: Generic

text: Preparasitic second-stage juveniles of M. chitwoodi were hatched in sterile tapwater. Total RNA was isolated according to TRIzol protocol, followed by mRNA isolation (Genovision mRNA beads). mRNA was used in first strand cDNA synthesis according to the Creator SMART cDNA library construction protocol, followed by cDNA amplification by 22 cycles of Long-Distance (LD) PCR, SfiI digestion, cDNA size fractionation, ligation into pDNR-LIB vector and transformation into electrocompetent TOP10 E. coli. This library was contributed by Erwin Roze (Erwin.Roze@wur.nl) of the Laboratory of Nematology at Wageningen University, Wageningen, Netherlands.

characteristic: description

text: preparasitic second-stage juveniles

text: electrocompetent TOP10 E. coli

text: Meloidogyne chitwoodi

text: Sfi IB (5'-GCCTC-3')

text: Sfi IA (5'-ATTAC-3')

text: whole worm

text: Meloidogyne chitwoodi J2 pDNR LIB

text: plasmid, chloramphenicol resistance

text: pDNR-LIB


text: live

text: Generic

text: cDNA primed using oligo-dT primer, directionally cloned into UDG sites of pAMP1. Size selected for insert sizes ranging from 0.2-1.6 kb. Normalized to Cot5. Primary library, non-amplified. Library constructed by M. Lovett. For more information on this library, please contact R. Tidwell (Washington University) or visit the COGENE website at http://hg.wustl.edu/COGENE/.

text: embryo, 8.5 weeks postconception

characteristic: lab-host

text: Homo sapiens

text: anterior tongue, pooled

text: COGENE 8.5 EAT

text: plasmid (ampicillin resistant)


text: Institute of Plant Genetics and Crop Plant Research (IPK)

text: live

text: Generic

characteristic: NCBI submission model

text: Sloop

characteristic: cultivar

text: Due to the cloning system used blue/white selection for recombinants is not 100 % reliable. Average insert size is 1.3 kb.

text: coleoptile, 1 day old

text: Hordeum vulgare

text: SalI (5-end of cDNA)

text: NotI (3-end of cDNA)

text: coleoptile

text: HH

text: pSPORT


text: live

text: Generic

characteristic: NCBI submission package

text: Sloop

text: cDNAs were directionally cloned using asymetric SfiI sites. Due to the cloning system used blue/white selection for recombinants is not 100 % reliable. Average insert size is 1.2 kb.

text: embryos isolated 8 days after pollination, corresponding to the globular stage of embryo development

text: lambda TripEx2 (Clontech)

text: Hordeum vulgare

text: embryo

characteristic: tissue-type

text: HJ

text: XL-10 Gold


text: Purdue University

text: live

text: Generic

text: Yecora Rojo (susceptible to Mycosphaerella graminicola)

text: Differential-display PCR clone derived from Mycosphaerella graminicola-inoculated wheat leaf tissue ; Kit: RNAimage (GenHunter, Nashville, TN). Total RNA from pathogen-infected leaf tissue was reverse-transcribed using one-base-oligo dT primers, followed by PCR using these primers in combination with random 10-mers. Differentially (or constitutively) expressed bands were reamplified using the same primers and cloned into the pCR 2.1 vector using the TA cloning kit (Invitrogen Corp., Carlsbad, CA) and sequenced.

text: seedling

text: Triticum aestivum

text: Leaf (X hours after pathogen inoculation)

text: Triticum aestivum cv. Yecora Rojo, Mycosphaerella graminicola-inoculated leaf tissue


text: Purdue University

text: live

text: Generic

text: Tadinia (resistant to Mycosphaerella graminicola)

text: Differential-display PCR clone derived from Mycosphaerella graminicola-inoculated wheat leaf tissue ; Kit: RNAimage (GenHunter, Nashville, TN). Total RNA from pathogen-infected leaf tissue was reverse-transcribed using one-base-oligo dT primers, followed by PCR using these primers in combination with arbitrary 13-mers. Differentially expressed bands were reamplified using the same primers and cloned into the pCR 2.1 vector using the TA cloning kit (Invitrogen Corp., Carlsbad, CA) and sequenced.

text: seedling

text: Triticum aestivum

text: Triticum aestivum cv. Tadinia, Mycosphaerella graminicola-inoculated leaf tissue


text: School of Life Science and Biotechnology, Korea University

text: live

text: Generic

text: After the suppression subtractive hybridization and MOS (Nucleic Acids Research, Vol 28, No 20, e90), cDNA fragments induced by wound treatment were cloned by TA cloning method, and screened by microarray.

text: Wound treatment & vegetative stage

text: Oryza minuta

characteristic: strain

text: leaf

text: Oryza minuta 101144 subtracted cDNA library

text: Phagemid


text: West China Medical Center, Sichuan University

text: live

text: Generic

text: Adult

text: Liver

characteristic: organ

text: Rattus norvegicus

text: male

characteristic: sex

text: Wistar

text: Liver

text: Rat hepatic hypercoagulation-related cDNA library


characteristic: External Id

text: Genencor Intl.

text: live

text: Generic

text: Mycelial culture grown from 24 hrs to 6 days with varying Carbon and Nitrogen sources and concentrations.

text: mycelia

characteristic: dev-stage

text: Trichoderma reesei

text: Not I/Sal I

text: T.reesei mycelial culture, Version 3 april

text: Plasmid


text: Henan Normal University

characteristic: INSDC center name

text: live

text: Generic

text: A rat regenerating liver cDNA library was constructed after partial hepatectomy eight hours by SSH(Suppression subtractive hybridization). We find a EST that is up-regulative in liver regeneration. Then we clone its 3-end by SMART RACE.

text: Rattus norvegicus

characteristic: organism

text: Sprague-Dawley

text: liver

text: Rat regenerating liver SMART RACE

text: pGEM-T


text: Henan Normal University

characteristic: INSDC first public

text: live

text: Generic

text: A rat regenerating liver cDNA library was constructed after partial hepatectomy eight hours by SSH(Suppression subtractive hybridization). The genes are down-regulative in liver regeneration.

text: Rattus norvegicus

text: Sprague-Dawley

text: liver

text: Rat genes down-regulated in liver regeneration

text: pGEM-T

characteristic: vector


text: The University of Georgia, Department of Plant Biology

characteristic: INSDC last update

text: live

text: Generic

text: The library was prepared from polyA+ RNA from seedlings grown in hydroponic culture. After 12 days, medium was supplemented with 1 mM abscisic acid (ABA), while leaves were misted with a solution of 1 mM ABA. Roots and leaves were harvested after 3, 6, 12, and 24 hr and material from all time points was combined prior to RNA isolation. Double-stranded cDNA was cloned unidirectionally into different DraIII sites of the pME18S-FL3 vector (5-prime DraIII site is CACTGTGTG, 3-prime DraIII site is CACCATGTG). XhoI excises the cDNA insert.

text: DH10B-T1 phage-resistant E. coli

text: Sorghum bicolor

text: XhoI

text: XhoI

text: Abscisic acid-treated seedlings

characteristic: title

text: Plasmid


text: Institute of Biochemistry, University of Goettingen

text: live

characteristic: INSDC status

text: Generic

text: ESTs from DDRT-PCR in peripheral blood

characteristic: description

text: Homo sapiens

text: male

text: Blood

text: Human DDRT-PCR Express Library


text: Indian Institute of Horticultural Research

text: live

text: Generic

characteristic: NCBI submission model

text: Alphonso

text: Used PCR based subtractive hybridization to isolate tissue specific mRNA from healthy and spongy tissue affected mango fruits.

text: Fruit

text: Mangifera indica

text: Fruit pulp

text: Mango fruit tissue express library


text: National Cancer Institute / NIH

text: live

text: Generic

text: Oligo-dT primed using primer 5'-TTTTTTTTTTTTTTTTVN-3', size-selected for average insert size 2.3 kb and normalized to ROT 5. This is a primary library enriched for full-length clones and constructed using the Cap-trapper method (Carninci, in preparation). Library constructed by M. Brownstein (NIMH/NHGRI, National Institutes of Health). Note: This is a NIH_MGC library.

text: DH10B TonA

characteristic: lab-host

text: placenta

text: Homo sapiens

text: alI-XhoI

text: BamH

text: Human Placenta

text: phagemid (ampicillin resistant

text: pBluescriptR


text: live

text: Generic

characteristic: NCBI submission package

text: Library is oligo-dT primed and directionally cloned using primer 5'-TTTTTTTTTTTTTTTTVN-3, size-selected for average insert size 2.3 kb and normalized to ROT 5. This is a primary library enriched for full-lenght clones and constructed using the Cap-trapper method (Carninci, in preparation). Library constructed by M. Brownstein (NIMH/NHGRI, National Institutes of Health). Note: this is a NIH_MGC Library.

text: DH10B TonA

text: placenta

text: Homo sapiens

text: alI-XhoI

text: BamH

text: pre-eclamptic placenta

text: phagemid (ampicillin resistant)

text: pBluescriptR


text: USDA-Agricultural Research Service

text: live

text: Generic

text: macrophage

characteristic: cell type

characteristic: cell-line

text: Primary library

text: adult

text: Ictalurus punctatus

text: Sal 1

text: Not 1

text: commercial

text: plasmid


text: NCSU

text: live

text: Generic

text: The library is from differentiating xylem from a single genotype, growing in Florida, collected in the beginning of October.

text: Adult (8 years)

text: Eucalyptus grandis

text: Eco RI

text: XhoI

text: Differentiating Xylem

characteristic: tissue-type

text: Differentiating xylem

text: BlueScript SK


text: University of Toronto

text: live

text: Generic

text: Midvein epidermal cells

text: Dixie Cream

characteristic: cultivar

text: Total RNA was isolated from cytoplasm extracted from individual midvein epidermal cells of cowpea plants resistant to the cowpea rust fungus at the stage of basidiospore infection prior to entry of the pathogen into the host cell lumen. Total RNA isolated from the extracted cytoplasm was reverse transcribed and PCR amplified according to the method of Iscove et al. (2002) Nature Biotechnology 20: 940-943. Clones were differentially screened using the amplified cDNAs generated from the micro-capillary extracted midvein epidermal cells of uninfected and rust-infected resistant and susceptible (cv. California Blackeye) plants.

text: 10-day-old plant

text: Vigna unguiculata

text: EcoR I

text: EcoR I

text: Primary leaf

text: cDNA library from rust fungus-infected, resistant, cowpea epidermal cells

text: Plasmid


characteristic: External Id

text: Scottish Crop Research Institute

text: live

text: Generic

text: Cloned in to tobacco mosaic virus-based vector pXNE

text: Root

characteristic: organ

text: Nicotiana benthamiana

text: Nicotiana benthamiana cDNA-GFP 3' fusion library


text: University of Edinburgh

characteristic: INSDC center name

text: live

text: Generic

text: neural(retinoic acid-treated embryoid bodies)

text: ES14/T (differentiated)

text: A subtracted cDNA library, derived from RA-treated embryoid bodies, enriched in sequences induced during neural determination. Library construction is described in Aubert, J., Dunstan, H., Chambers, I., Smith, A. (2002) Functional gene screening in embryonic stem cells implicates Wnt antagonism in neural differentiation. Nature Biotech. 20:1240-45.

text: Mus musculus

text: male

characteristic: sex

characteristic: strain

text: embryoid bodies (retinoic acid-treated)

text: Embryoid body +RA(-RA subtracted) (J.Aubert)

text: Plasmid

text: pCRII-topo


text: Fukuoka University School of Medicine

characteristic: INSDC first public

text: live

text: Generic

text: hybridoma

text: Homo sapiens

characteristic: organism

text: Homo sapiens hybridoma


text: Genoscope - Centre National de Sequencage

characteristic: INSDC last update

text: live

text: Generic

text: RAMOS CELL LINE

text: 1st strand cDNA was primed with a NotI-oligo(dT) primer. Five prime end enriched, double-strand cDNA was digested with Not I and cloned into the Not I and EcoR V sites of the pCMVSPORT 6 vector. Library was normalized.

text: Homo sapiens

text: Homo sapiens B CELLS (RAMOS CELL LINE) COT 25-NORMALIZED

characteristic: title


text: live

characteristic: INSDC status

text: Generic

characteristic: description

text: Homo sapiens

text: NEUROBLASTOMA COT 50-NORMALIZED

text: Homo sapiens NEUROBLASTOMA COT 50-NORMALIZED


text: Washington University School of Medicine

text: live

text: Generic

characteristic: NCBI submission model

text: Sporozoites were obtained from in vitro sporulated and excysted oocysts of E.tenella grown in chickens. cDNA was synthesized from poly mRNA using an oligo-dT primer containing a XhoI site. Following second strand synthesis, EcoRI adapters were ligated to the cDNA and products were size-selected on sephacryl S500. The cDNA were ligated to EcoRI/XhoI prepared lambda ZapII (Stratagene). The primary library was mass excised using ExAssist helper phage (Stratagene). The phagemids were preciptated with PEG 8000, extracted with phenol/chloroform and electroporated into DH10B cells. The library may contain a small percentage of host or bacterial contaminants.

characteristic: dev-stage

text: E.coli DH10B (GeneHog, Invitrogen,Inc)

characteristic: lab-host

text: Eimeria tenella

text: EcoRI

text: XhoI

text: Eimeria tenella S5-2 Excised cDNA

text: phagemid (Amp. resistant)

text: Bluescript SK-

characteristic: vector


text: UC Davis, Plant Pathology

text: live

text: Generic

characteristic: NCBI submission package

text: Cabernet Sauvignon

text: CAB3 is a cDNA library of Vitis vinifera 'Cabernet Sauvignon' Clone 8 berries. Samples were collected after berry set from field-grown vines during stage I of berry growth, 17 days after full bloom. The average berry size was 6 milimeters. Sampled vines were located at the University of California, Davis, Experimental Vineyard. cDNAs were made by oligo-dT priming and directionally cloned. 5'and 3' adaptors were used in cloning as follows: 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' and 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)NN-3'. Library was constructed using the Clontech Creator SMART kit and size-selected to contain the 0.5-3 kb size fraction.

text: Berry stage I

text: Berry

text: Vitis vinifera

text: SfiI

text: SfiI

text: Hermaphrodite

text: Cabernet Sauvignon Berry Stage I - CAB3

text: Plasmid

text: pDNR


text: UC Davis, Plant Pathology

text: live

text: Generic

text: Cabernet Sauvignon

characteristic: cultivar

text: CAB4 is a cDNA library of Vitis vinifera cv. 'Cabernet Sauvignon' Clone 8 berries. Sampled berries were collected from field-grown vines during stage II of berry growth (berries were green and hard) at approximately 60 days after full bloom.The average berry size was 9 milimeters. Sampled vines were located at the University of California, Davis, Experimental Vineyard. cDNAs were made by oligo-dT priming and directionally cloned. 5'and 3' adaptors were used in cloning as follows: 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' and 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)NN-3'. Library was constructed using the Clontech Creator SMART kit and size-selected to contain the 0.5-3 kb size fraction.

text: Berry on stage II, 9 mm

text: Berry

text: Vitis vinifera

text: SfiI

text: SfiI

text: Hermaphrodite

text: Cabernet Sauvignon Berry - CAB4

text: Plasmid

text: pDNR


characteristic: External Id

text: UC Davis, Plant Pathology

text: live

text: Generic

text: Cabernet Sauvignon

text: CAB7 is a cDNA library of Cabernet Sauvignon Clone 8 berries. Samples were collected post-veraison from field-grown vines during stage III of berry growth at 89 days after full bloom. Berries soluble solid concentration ranged between 18-19 brix. Sampled vines were located at the University of California, Davis, Experimental Vineyard. cDNAs were made by oligo-dT priming and directi onally cloned. 5'and 3' adaptors were used in cloning as follows: 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' and 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)NN-3'. Library was constructed using the Clontech Creator SMART kit and size-selected to contain the 0.5-3 kb size fraction.

text: Post-Veraison, 18-19 brix

text: Berry

text: Vitis vinifera

text: SfiI

text: SfiI

text: Hermaphrodite

text: Cabernet Sauvignon Berry Post-Veraison - CAB7

text: Plasmid

text: pDNR


text: National Cancer Institute / NIH

text: live

text: Generic

text: Library is oligo-dT primed and directionally cloned (EcoRV site is destroyed upon cloning). Average insert size 1.1 kb. Library was constructed by (Invitrogen). Note: this is a NIH_MGC Library.

text: DH10B-Ton A ( T1 and T5 phage resistances)

text: brain

characteristic: organ

text: Homo sapiens

text: EcoRV (destroyed)

text: NotI

text: Pituitary

characteristic: tissue-type

text: plasmid (ampicillin resistant)


characteristic: INSDC center name

text: live

text: Generic

text: Homo sapiens

text: NEUROBLASTOMA COT 25-NORMALIZED

text: Homo sapiens NEUROBLASTOMA COT 25-NORMALIZED


text: live

text: Generic

text: Homo sapiens

text: PLACENTA COT 25-NORMALIZED

text: Homo sapiens PLACENTA COT 25-NORMALIZED


text: live

text: Generic

characteristic: cell type

text: JURKAT

characteristic: cell-line

text: Homo sapiens

text: Homo sapiens T CELLS (JURKAT CELL LINE) COT 10-NORMALIZED


characteristic: INSDC first public

text: live

text: Generic

text: Homo sapiens

characteristic: organism

text: NEUROBLASTOMA COT 10-NORMALIZED

text: Homo sapiens NEUROBLASTOMA COT 10-NORMALIZED


characteristic: INSDC last update

text: live

text: Generic

text: HELA CELLS COT 25-NORMALIZED

text: HELA

text: Homo sapiens

text: Homo sapiens HELA CELLS COT 25-NORMALIZED

characteristic: title


text: Incyte Corporation

text: live

characteristic: INSDC status

text: Generic

text: RT-PCR was performed using gene-specific primers flanking the open-reading frame. PCR products were subcloned into pDrive Cloning Vector and sequenced completely using M13 forward and reverse primers. Sequencing gaps were closed by re-sequencig using primers flanking the gapped areas.

characteristic: description

text: Homo sapiens

text: Single gene library

text: Plasmid

text: pDrive Cloning Vector


text: live

text: Generic

characteristic: NCBI submission model

text: Oligo-dT primed cDNA was produced from intestine (two independent samples) and liver using 5'-GAGAGAGAGAAGGATCCAAXXXXXXTTTTTTTTTTTTTTTTVN-3' with the following tags substituting for XXXXXX: CAAGAG (tag C04): liver; CGGTAT (tag C12): intestine; and CGTATG (tag D01): intestine. cDNA was pooled and size- selected for a 1.6 kb average insert. Library was constructed using the Cap- Trapper method as described in Genomics 2001: 77(1-2)79-90. Library donated by M. Pack, M.D. (University of Pennsylvania School of Medicine).

text: DH10B (phage-resistant)

text: Danio rerio

text: XhoI

text: BamHI

text: intestine (2 samples) and liver (pooled)

text: plasmid (ampicillin resistant)

text: pBluescriptR


text: Oklahoma State University

text: live

text: Generic

text: Bos taurus

text: Embryo

text: bovine in vivo blastocyst cDNA


text: Northeast Forestry University

text: live

text: Generic

text: Tamarix androssowii

text: leaf

text: Tamarix androssowii leaf


text: Texas A&M University

text: live

text: Generic

text: mixed sexual development

text: Two mating types were co-cultivated over a filter paper on oatmeal agar medium. After three days at 25 C plates were transfered to 21 C. Perithecia with asci and ascospores formed at the begining of the third week. Material was collected by scraping tissue from the filter paper. Sequences were processed by one of two methods. Where a full-length alignment to the M. grisea genome sequence was available, the EST sequence was trimed according to the alignment, otherwise sequence quality was assessed using phredPhrap version 991019 and trimmed according to phd files (0.05) and for vector seqs.

text: asci, ascospores, perithecia, mycelium

characteristic: dev-stage

text: Pyricularia grisea

text: EcoRI

text: XhoI

text: Mat1-2 and Mat1-1 mixted culture

characteristic: sex

text: 4091-5-8 X 4136-4-3

characteristic: strain

text: Mated culture

text: Phagemid

text: pBluescriptSK


text: Texas A&M University

text: live

text: Generic

characteristic: NCBI submission package

text: germinated conidia

text: Mycelial library of natural isolate from Thailand in which the most abundant clones were subtracted. Library obtained from Bayer Crop Sciences. Sequences were processed by one of two methods. Where a full-length alignment to the M. grisea genome sequence was available, the EST sequence was trimed according to the alignment, otherwise sequence quality was assessed using phredPhrap version 991019 and trimmed according to phd files (0.05) and for vector seqs.

text: Pyricularia grisea

text: EcoRI

text: XhoI

text: Mat1-2 hermaphrodite

text: Magnaporthe grisea subtracted mycelial library

text: Phagemid

text: pBluescriptSK

characteristic: vector


characteristic: External Id

text: USDA/ARS, Crop Protection and Management Research Unit

text: live

text: Generic

characteristic: cultivar

text: cDNA library was constructed from peanut cultivar C34-24(F439-16-10-3XPI203396). C34-24 has resistance to leaf spot diseases and tomato spot wilt virus. Newly expanded leaves were collected from different plants, and placed into liquid N2 immediately and stored in -80oC freezer. Total RNA was isolated with TRIZOL-Reagent ultrapure(GIBCOBRL). mRNA was extracted and purified from total RNA (Promega). cDNA synthesis and library construction were followed the protocol of ZAP-cDNA Gigapack III Gold cloning kit (Stratagene). The cDNA above 300bp were collected after size-fraction. The inserts were directionally cloned into Uni-ZAP XR vector using XhoI EcoRI sites adapters. The lamda library was packed into phages using Gigapack III Gold (Stratagene). The un-amplified library was used to excise pBluescript phagemids from the Uni-ZAP XR vector, and the phagemids was used to transform the host bacteria SOLR. The library was constructed by Dr. Meng Luo and Dr. Phat Dang.

text: newly expanded leaves 100 days after planting

characteristic: lab-host

text: Arachis hypogaea

text: EcoRI

text: XhoI

text: leaf

text: USDA-Tifton Peanut Leaf cDNA Library (UTPL)

text: Uni-ZAP XR


characteristic: INSDC center name

text: live

text: Generic

text: cDNA library was constructed from peanut cultivar A13 (NCV11XAR4). A13 has resistance to Aspergillus infection and drought tolerance. The immature pods that developed to R6 stage were collected from different plants, and placed into liquid N2 immediately and stored in -80oC freezer. Total RNA was isolated with TRIZOL-Reagent ultrapure(GIBCOBRL). mRNA was extracted and purified from total RNA (Promega). cDNA synthesis and library construction followed the protocol of by ZAP-cDNA Gigapack III Gold cloning kit (Stratagene). The cDNA above 500bp were collected after size-fraction. The inserts were directionally cloned into Uni-ZAP XR vector using XhoI EcoRI sites adapters. The lamda library was packed into phages using Gigapack III Gold (Stratagene). The un-amplified library was used to excise pBluescript phagemids from the Uni-ZAP XR vector, and the phagemids was used to transform the host bacteria SOLR. The library was constructed by Dr. Meng Luo and Dr. Phat Dang.

text: Arachis hypogaea

text: EcoRI

text: XhoI

text: Immature pods

characteristic: tissue-type

text: USDA-Tifton Peanut Immature Pod cDNA Library (UTPP)

text: Uni-ZAP XR


text: GuangZhou Medical College

characteristic: INSDC first public

text: live

text: Generic

text: mesenchymal stem cell

characteristic: cell type

text: Rattus norvegicus

text: male

text: Sprague-Dawley

text: Neuron-like cell cDNA differentiated from MSC


text: National Institute for Plant Genome Research

characteristic: INSDC last update

text: live

text: Generic

text: PUSA Pragati (Dharwar)

text: seedling

text: Cicer arietinum

characteristic: organism

text: subtracted cDNA library of drought-stressed chickpea


text: Genoscope - Centre National de Sequencage

text: live

characteristic: INSDC status

text: Generic

text: 1st strand cDNA was primed with a NotI-oligo(dT) primer. Five prime end enriched, double-strand cDNA was digested with Not I and cloned into the Not I and EcoRV sites of the pCMVSPORT 6 vector. Library was not normalized.

text: Homo sapiens

text: NEUROBLASTOMA

text: Homo sapiens NEUROBLASTOMA

characteristic: title


text: live

text: Generic

characteristic: NCBI submission model

characteristic: description

text: Homo sapiens

text: PLACENTA

text: Homo sapiens PLACENTA


text: Shizuoka University

text: live

text: Generic

text: initiation stage of rind peeling

characteristic: dev-stage

text: Citrus unshiu

text: albedo

text: Citrus unshiu albedo initiation stage of rind peeling


text: National Cancer Institute / NIH

text: live

text: Generic

text: LIBR_PRIMING - oligo dT; METHOD - full-length enriched; Embryonic Stem Cells H1; LIBR PROVIDER - Bradfield

text: DH10B TonA

text: Homo sapiens

text: embryonic stem cells, WA01, passage 38

text: phagemid (Kanamycin resistant)


text: National Cancer Institute / NIH

text: live

text: Generic

text: LIBR_PRIMING - oligo dT; METHOD - full-length enriched; LIBR PROVIDER - Bradfield

text: DH10B TonA

text: Homo sapiens

text: embryonic trophoblasts, made from WA01 stem cells

text: phagemid (Kanamycin resistant)


text: Virginia Bioinformatics Institute

text: live

text: Generic

characteristic: cell-line

text: Agriculture canada Phytophthora sojae EST project

text: Phytophthora sojae

text: EcoRI

text: XhoI

text: infected hypocotyl

text: psHA: Soybean host 48 hrs post infection

text: Phagemid

text: pBK-CMV


text: Virginia Bioinformatics Institute

text: live

text: Generic

characteristic: NCBI submission package

text: USDA-IFAFS:Expression of Phytophthora sojae genes during infection and propagation.

text: 48 hour post infection

text: Phytophthora sojae

text: EcoRI

text: XhoI

text: infected host tissue

text: psHB: Infected hypocotyl soybean host. 48 hrs post infection

text: Phagemid

text: pBK-CMV


text: Inner Mongolian Agricultural University

text: live

text: Generic

text: Capra hircus

text: skin

text: Cashmere goat skin cDNA library


text: Kangwon National Univ.

text: live

text: Generic

text: Spuriopimpinella brachycarpa

text: Pimpinella brachycarpa cDNA AFLP fragment


text: live

text: Generic

text: fetal

text: brain

characteristic: organ

text: Homo sapiens

text: FETAL BRAIN

text: Homo sapiens FETAL BRAIN

characteristic: vector


characteristic: External Id

text: Oklahoma State University

text: live

text: Generic

text: Embryonic

text: A cDNA expression library was constructed in the vector pEXP1 containing the strong human cytomegalovirus major immediate early promoter/enhancer (CMVIE) (Clontech, Palo Alto, CA) using cultured embryonic I. scapularis IDE8 cells-derived poly(A)+ RNA. The cDNA library contained 4.4 x 106 independent clones and a titer of approximately 1010 cfu/ml with more than 93% of the clones with cDNA inserts. The average cDNA size was 1.7 kb (0.5-4.0 kb).

text: Escherichia coli

text: Ixodes scapularis

text: cDNA expression library from I. scapularis IDE8 cells

text: Plasmid


text: Institute of Plant Genetics and Crop Plant Research (IPK)

text: live

text: Generic

text: Ingrid BC mlo-5

characteristic: cultivar

text: Approximately 5 % of the clones correspond to cDNA from the fungi B. graminis hordei and tritici, respectively. Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable. Average insert size is 1.2 kb

text: 7 d after germination

characteristic: lab-host

text: Hordeum vulgare

text: EcoRI (5'-end of cDNA)

text: XhoI (3'-end of cDNA)

text: leaf epidermis, 6 h and 24 h post inoculation with Blumeria graminis

text: HO

text: pBluescript SK+


characteristic: INSDC center name

text: live

text: Generic

text: Homo sapiens

text: THYMUS

characteristic: tissue-type

text: Homo sapiens THYMUS


characteristic: INSDC first public

text: live

text: Generic

text: RAMOS CELL LINE

text: Homo sapiens

text: B CELLS (RAMOS CELL LINE)

text: Homo sapiens B CELLS (RAMOS CELL LINE)