text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UKS47 from Kampala, Uganda originated from a patient sufferring from severe malaria. Erythrocytes were separated from other blood constituents, parasites enriched and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Ring

text: Human

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UKS47 Pf Ring stage var gene cDNA

text: pcrII-TOPO


status: PUBLIC

characteristic: External Id

text: University of Bern

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UKS47 from Kampala, Uganda originated from a patient sufferring from severe malaria. Erythrocytes were separated from other blood constituents and cultured until parasites matured into trophozoites. Trophozoites were enriched and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

characteristic: description

text: Trophozoite

tag: attribute

characteristic: dev-stage

characteristic: isolate

text: Human

characteristic: lab-host

text: Plasmodium falciparum

characteristic: organism

text: Blood

characteristic: tissue-type

text: Ugandan isolate UKS47 Pf Trophozoite stage var gene cDNA

characteristic: title

text: pcrII-TOPO

characteristic: vector


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UKS5 from Kampala, Uganda originated from a patient sufferring from severe malaria. Erythrocytes were separated from other blood constituents, parasites enriched and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Ring

text: Human

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UKS5 Pf Ring stage var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UKS7 from Kampala, Uganda originated from a patient sufferring from severe malaria. Erythrocytes were separated from other blood constituents, parasites enriched and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Ring

text: Human

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UKS7 Pf Ring stage var gene cDNA

text: pcrII-TOPO


text: University of Bern

text: live

text: Generic

text: Blood samples were drawn from Ugandan children with mild or severe malaria. Isolate UKS7 from Kampala, Uganda originated from a patient sufferring from severe malaria. Erythrocytes were separated from other blood constituents and cultured until parasites matured into trophozoites. Trophozoites were enriched and total RNA extracted. Reverse transcription with random hexamer oligonucleotides and DNAse treatment gave single stranded cDNA. Three different pairs of primers (alpha-AF/alpha-BR, nDBLf/nDBLr, nDBLf/alpha-BR) were used to PCR amplify the var gene/PfEMP1 DBL1alpha domain from cDNA. A set of 48 clones were obtained from each reaction and sequenced once from each direction.

text: Trophozoite

text: Human

text: Plasmodium falciparum

text: Blood

text: Ugandan isolate UKS7 Pf Trophozoite stage var gene cDNA

text: pcrII-TOPO


text: National Research Centre

text: live

text: Generic

text: Polled organs

characteristic: organ

text: Oreochromis niloticus

text: male and female

characteristic: sex

text: Liver, kidney, gills and intestin

text: Fish immune genes cDNA library

text: Plasmid,YAC


characteristic: External Id

text: Tottori University

text: live

text: Generic

text: Nijisseiki

characteristic: cultivar

text: Pyrus pyrifolia

text: leaf

text: Pyrus pyrifolia cv. Nijisseiki leaf


text: University of Tokyo

characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: adult

text: Ciona intestinalis

text: whole

text: Ciona intestinalis whole adult oligo-capped cDNA library


text: Genoscope - Centre National de Sequencage

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: genotype: UF676; SSH library from non attacked-attacked young shoots by Sahlbergella singularis

text: Theobroma cacao

text: Shoot


text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: genotype: UF676; SSH library from attacked-non attacked young shoots by Sahlbergella singularis

text: Theobroma cacao

text: Shoot


text: live

text: Generic

text: genotype: Scavina6; complete disc of stems 1 cm diameter

characteristic: description

text: Theobroma cacao

text: Stem


text: live

text: Generic

text: genotype: ICS1; fermented seeds during 32 to 40H

text: Theobroma cacao

text: Seed


text: live

text: Generic

text: genotype: Scavina6; SSH library from infected/non infected (and reverse sens) shoot tissues by Moniliophthora perniciosa less than 18 days after inoculation

text: Theobroma cacao

text: Shoot


text: live

text: Generic

text: genotype: ICS1; testa with pulp from mature seeds

text: Theobroma cacao

text: Seed


text: live

text: Generic

text: genotype: Scavina6; embryogenic and non embryogenic callus in vitro culture

text: Theobroma cacao

text: Callus


text: live

text: Generic

text: genotype: Jaca; SSH library from non infected-infected stems by Ceratocystis fimbriata

text: Theobroma cacao

text: Stem


text: live

text: Generic

text: genotype: Scavina6; SSH library from infected-non infected shoot tissues by Moniliophthora perniciosa between 18 to 120 days after inoculation

text: Theobroma cacao

text: Shoot


text: live

text: Generic

text: genotype: Jaca; SSH library from infected-non infected stems by Ceratocystis fimbriata

text: Theobroma cacao

text: Stem


text: live

text: Generic

text: genotype: ICS1; fermented testa during 6 to 40H

text: Theobroma cacao

text: Testa


text: live

text: Generic

text: genotype: P7; young wilted cherelles 7 to 10 days after pollination

text: Theobroma cacao

text: Cherelle


text: live

text: Generic

text: genotype: 33-49; testa from seeds fermented between 6H and 4 days

text: Theobroma cacao

text: Seed


text: live

text: Generic

text: genotype: Scavina6; bark and cambium part of woods

text: Theobroma cacao

text: Wood


text: live

text: Generic

text: genotype: ICS1; fermented seeds during 6 to 26H

text: Theobroma cacao

text: Seed


text: Central South University of Forestry and Technology

text: live

text: Generic

text: Hunan Meihe in China

text: Material: mature foliage from Ginkgo, Hunan Meihe cultivar in China. mRNA was extracted using Oligotex mRNA midi kit. First-Strand cDNA was synthesized by SuperScipt TM Rnase H-Reverse Transcriptase. The double cDNA was inserted into pBluescript SK(+) XR. The library consisted of 8.12x105 recombinants with the insert length of 500-2 000 bp, and the recombination efficiency was 90 %.

text: mature

characteristic: dev-stage

text: leaf

text: Ginkgo biloba

text: Xho I

text: EcoR I

text: female

text: Ginkgo mature foliage Library

text: pBluescript SK


text: Central Food Technological Research Institute

text: live

text: Generic

text: Haematococcus lacustris

characteristic: strain

text: Haematococcus pluvialis SAG-19a


text: University of California

text: live

text: Generic

text: UCR 5301

text: Seeds of UCR 5301 were from a single plant with a long history of single seed descent (Ehlers) from a wild cowpea accession. The types of tissue were: 1) germinated seed hypocotyl and primary root, 2) germinated seed epicotyl and foliage leaves, 3 & 4) young trifoliate leaves and apical meristem (one well watered, one drought stressed). For germinated seed samples (1 & 2), seeds were surface sterilized 5 minutes in 1% sodium hypochlorite with 0.01% Tween 20, rinsed with water, germinated on sterile filter paper with water containing 100 units/ml nystatin and 50 microgram/ml cefotaxime, incubated 4-5 days in the dark at room temperature (Fenton). For samples 3 & 4, seeds were planted in 3 gallon pots and kept watered in a glasshouse until plants were ~25 cm in height (~3 weeks)(Fenton). For drought stress (sample 4), water was withheld until visible signs of drought stress occurred (reduced growth relative to watered plants, wilted leaves, less green color), generally ~3 weeks. Total RNA was purified using a TRIzol method, except Qiagen RNeasy for hypocotyls & root (Fenton). RNA was pooled to establish one complex RNA sample with a total of 500 microgram, as follows: 167 microgram of hypocotyl & primary root (sample 1), 167 microgram of epicotyl and foliage leaves (sample 2) and 87 microgram each of stressed and unstressed young trifoliate leaves and apical meristem (samples 3 & 4)(Fenton). PolyA RNA was purified using PolyATrack mRNA Isolation System IV (Promega)(Fenton). 2.0 microgram of poly(A) mRNA was used for cDNA synthesis. A primary cDNA library with a titer of 25.6 million cfu (Close) was produced using a SuperScript Plasmid System with Gateway Technology for cDNA Synthesis and Cloning (Invitrogen)(Fenton). Plating and sequencing was performed at the US Department of Energy Joint Genome Institute Sequence Production Facility, Walnut Creek California (Bristow, Wang, Lucas, Lindquist, Pennacchio). The library was plated onto agarose plates containing ampicillin (254 mm plates from Teknova, Hollister, CA) at a density of approximately 1000 colonies per plate. Plates were grown at 37 degrees Celsius for 18 hours, then individual colonies were picked and each used to inoculate a well containing LB media with ampicillin in a 384 well plate (Nunc, Rochester, NY). 384 well plates were grown at 37 degrees Celsius for 18 hours. Plasmid DNA for sequencing was produced by rolling circle amplification (Templiphi, GE Healthcare, Piscataway, NJ). Subclone inserts were sequenced from both ends using primers complementary to the flanking pCMV-SPORT6 vector sequence and Big Dye terminator chemistry and then run on ABI 3730 instruments (ABI, Foster City, CA). Sequencing protocols can be found in Detter et al (2002, Genomics 80:691-698) and at http://www.jgi.doe.gov/sequencing/protocols/index.html. Trace files were downloaded (Close) and then processed (Wanamaker) using the HarvEST pipeline (http://harvest.ucr.edu) to remove vector and cloning oligo sequences and various contaminants, and to trim to a high quality region. Sequences that retained a phred 17 region of at least 100 bases were deposited to GenBank with annotations (Close).

text: Seedling

text: E. coli DH10B

characteristic: lab-host

text: Vigna unguiculata

characteristic: organism

text: SalI

text: NotI

text: mixed

text: Cowpea UCR 5301 Mixed Tissue and Conditions cDNA Library UCRVU06-1-2

text: Plasmid


text: University of California

text: live

text: Generic

text: PI 418979

text: Seeds of PI 418979 were from a single plant with a long history of single seed descent (Ehlers) from China. The types of tissue were: 1) germinated seed hypocotyl and primary root, 2) germinated seed epicotyl and foliage leaves, 3 & 4) young trifoliate leaves and apical meristem (one well watered, one drought stressed). For germinated seed samples (1 & 2), seeds were surface sterilized 5 minutes in 1% sodium hypochlorite with 0.01% Tween 20, rinsed with water, germinated on sterile filter paper with water containing 100 units/ml nystatin and 50 microgram/ml cefotaxime, incubated 4-5 days in the dark at room temperature (Fenton). For samples 3 & 4, seeds were planted in 3 gallon pots and kept watered in a glasshouse until plants were ~25 cm in height (~3 weeks)(Fenton). For drought stress (sample 4), water was withheld until visible signs of drought stress occurred (reduced growth relative to watered plants, wilted leaves, less green color), generally ~3 weeks. Total RNA was purified using a TRIzol method, except Qiagen RNeasy for hypocotyls & root (Fenton). RNA was pooled to establish one complex RNA sample with a total of 500 microgram, as follows: 167 microgram of hypocotyl & primary root (sample 1), 167 microgram of epicotyl and foliage leaves (sample 2) and 87 microgram each of stressed and unstressed young trifoliate leaves and apical meristem (samples 3 & 4)(Fenton). PolyA RNA was purified using PolyATrack mRNA Isolation System IV (Promega)(Fenton). 2.0 microgram of poly(A) mRNA was used for cDNA synthesis. A primary cDNA library with a titer of 15 million cfu (Close) was produced using a SuperScript Plasmid System with Gateway Technology for cDNA Synthesis and Cloning (Invitrogen)(Fenton). Plating and sequencing was performed at the US Department of Energy Joint Genome Institute Sequence Production Facility, Walnut Creek California (Bristow, Wang, Lucas, Lindquist, Pennacchio). The library was plated onto agarose plates containing ampicillin (254 mm plates from Teknova, Hollister, CA) at a density of approximately 1000 colonies per plate. Plates were grown at 37 degrees Celsius for 18 hours, then individual colonies were picked and each used to inoculate a well containing LB media with ampicillin in a 384 well plate (Nunc, Rochester, NY). 384 well plates were grown at 37 degrees Celsius for 18 hours. Plasmid DNA for sequencing was produced by rolling circle amplification (Templiphi, GE Healthcare, Piscataway, NJ). Subclone inserts were sequenced from both ends using primers complementary to the flanking pCMV-SPORT6 vector sequence and Big Dye terminator chemistry and then run on ABI 3730 instruments (ABI, Foster City, CA). Sequencing protocols can be found in Detter et al (2002, Genomics 80:691-698) and at http://www.jgi.doe.gov/sequencing/protocols/index.html. Trace files were downloaded (Close) and then processed (Wanamaker) using the HarvEST pipeline (http://harvest.ucr.edu) to remove vector and cloning oligo sequences and various contaminants, and to trim to a high quality region. Sequences that retained a phred 17 region of at least 100 bases were deposited to GenBank with annotations (Close).

text: Seedling

text: E. coli DH10B

text: Vigna unguiculata

text: SalI

text: NotI

text: mixed

characteristic: tissue-type

text: Cowpea PI 418979 Mixed Tissue and Conditions cDNA Library UCRVU10-1-2

characteristic: title

text: Plasmid


text: University of California

text: live

text: Generic

text: UCR 41

text: Seeds of UCR 41 were from a single plant with a long history of single seed descent (Ehlers) from Nicaragua. The types of tissue were: 1) germinated seed hypocotyl and primary root, 2) germinated seed epicotyl and foliage leaves, 3 & 4) young trifoliate leaves and apical meristem (one well watered, one drought stressed). For germinated seed samples (1 & 2), seeds were surface sterilized 5 minutes in 1% sodium hypochlorite with 0.01% Tween 20, rinsed with water, germinated on sterile filter paper with water containing 100 units/ml nystatin and 50 microgram/ml cefotaxime, incubated 4-5 days in the dark at room temperature (Fenton). For samples 3 & 4, seeds were planted in 3 gallon pots and kept watered in a glasshouse until plants were ~25 cm in height (~3 weeks)(Fenton). For drought stress (sample 4), water was withheld until visible signs of drought stress occurred (reduced growth relative to watered plants, wilted leaves, less green color), generally ~3 weeks. Total RNA was purified using a TRIzol method, except Qiagen RNeasy for hypocotyls & root (Fenton). RNA was pooled to establish one complex RNA sample with a total of 500 microgram, as follows: 167 microgram of hypocotyl & primary root (sample 1), 167 microgram of epicotyl and foliage leaves (sample 2) and 87 microgram each of stressed and unstressed young trifoliate leaves and apical meristem (samples 3 & 4)(Fenton). PolyA RNA was purified using PolyATrack mRNA Isolation System IV (Promega)(Fenton). 2.0 microgram of poly(A) mRNA was used for cDNA synthesis. A primary cDNA library with a titer of 18 million cfu (Close) was produced using a SuperScript Plasmid System with Gateway Technology for cDNA Synthesis and Cloning (Invitrogen)(Fenton). Plating and sequencing was performed at the US Department of Energy Joint Genome Institute Sequence Production Facility, Walnut Creek California (Bristow, Wang, Lucas, Lindquist, Pennacchio). The library was plated onto agarose plates containing ampicillin (254 mm plates from Teknova, Hollister, CA) at a density of approximately 1000 colonies per plate. Plates were grown at 37 degrees Celsius for 18 hours, then individual colonies were picked and each used to inoculate a well containing LB media with ampicillin in a 384 well plate (Nunc, Rochester, NY). 384 well plates were grown at 37 degrees Celsius for 18 hours. Plasmid DNA for sequencing was produced by rolling circle amplification (Templiphi, GE Healthcare, Piscataway, NJ). Subclone inserts were sequenced from both ends using primers complementary to the flanking pCMV-SPORT6 vector sequence and Big Dye terminator chemistry and then run on ABI 3730 instruments (ABI, Foster City, CA). Sequencing protocols can be found in Detter et al (2002, Genomics 80:691-698) and at http://www.jgi.doe.gov/sequencing/protocols/index.html. Trace files were downloaded (Close) and then processed (Wanamaker) using the HarvEST pipeline (http://harvest.ucr.edu) to remove vector and cloning oligo sequences and various contaminants, and to trim to a high quality region. Sequences that retained a phred 17 region of at least 100 bases were deposited to GenBank with annotations (Close).

text: Seedling

text: E. coli DH10B

text: Vigna unguiculata

text: SalI

text: NotI

text: mixed

text: Cowpea UCR 41 Mixed Tissue and Conditions cDNA Library UCRVU11-1-2

text: Plasmid

characteristic: vector


text: University of California

text: live

text: Generic

text: UCR 2563

text: Seeds of UCR 2563 were from a single plant with a long history of single seed descent (Ehlers)from Brazil. The types of tissue were: 1) germinated seed hypocotyl and primary root, 2) germinated seed epicotyl and foliage leaves, 3 & 4) young trifoliate leaves and apical meristem (one well watered, one drought stressed). For germinated seed samples (1 & 2), seeds were surface sterilized 5 minutes in 1% sodium hypochlorite with 0.01% Tween 20, rinsed with water, germinated on sterile filter paper with water containing 100 units/ml nystatin and 50 microgram/ml cefotaxime, incubated 4-5 days in the dark at room temperature (Fenton). For samples 3 & 4, seeds were planted in 3 gallon pots and kept watered in a glasshouse until plants were ~25 cm in height (~3 weeks)(Fenton). For drought stress (sample 4), water was withheld until visible signs of drought stress occurred (reduced growth relative to watered plants, wilted leaves, less green color), generally ~3 weeks. Total RNA was purified using a TRIzol method, except Qiagen RNeasy for hypocotyls & root (Fenton). RNA was pooled to establish one complex RNA sample with a total of 500 microgram, as follows: 167 microgram of hypocotyl & primary root (sample 1), 167 microgram of epicotyl and foliage leaves (sample 2) and 87 microgram each of stressed and unstressed young trifoliate leaves and apical meristem (samples 3 & 4)(Fenton). PolyA RNA was purified using PolyATrack mRNA Isolation System IV (Promega)(Fenton). 2.0 microgram of poly(A) mRNA was used for cDNA synthesis. A primary cDNA library with a titer of 15.7 million cfu (Close) was produced using a SuperScript Plasmid System with Gateway Technology for cDNA Synthesis and Cloning (Invitrogen)(Fenton). Plating and sequencing was performed at the US Department of Energy Joint Genome Institute Sequence Production Facility, Walnut Creek California (Bristow, Wang, Lucas, Lindquist, Pennacchio). The library was plated onto agarose plates containing ampicillin (254 mm plates from Teknova, Hollister, CA) at a density of approximately 1000 colonies per plate. Plates were grown at 37 degrees Celsius for 18 hours, then individual colonies were picked and each used to inoculate a well containing LB media with ampicillin in a 384 well plate (Nunc, Rochester, NY). 384 well plates were grown at 37 degrees Celsius for 18 hours. Plasmid DNA for sequencing was produced by rolling circle amplification (Templiphi, GE Healthcare, Piscataway, NJ). Subclone inserts were sequenced from both ends using primers complementary to the flanking pCMV-SPORT6 vector sequence and Big Dye terminator chemistry and then run on ABI 3730 instruments (ABI, Foster City, CA). Sequencing protocols can be found in Detter et al (2002, Genomics 80:691-698) and at http://www.jgi.doe.gov/sequencing/protocols/index.html. Trace files were downloaded (Close) and then processed (Wanamaker) using the HarvEST pipeline (http://harvest.ucr.edu) to remove vector and cloning oligo sequences and various contaminants, and to trim to a high quality region. Sequences that retained a phred 17 region of at least 100 bases were deposited to GenBank with annotations (Close).

text: Seedling

text: E. coli DH10B

text: Vigna unguiculata

text: SalI

text: NotI

text: mixed

text: Cowpea UCR 2563 Mixed Tissue and Conditions cDNA Library UCRVU12-2-2

text: Plasmid


text: Institute of Genetic and Developmental Biology, Chinese Academy of Sciences

text: live

text: Generic

text: Solanum nigrum

text: seedling

text: Solanum nigrum seedlings differential display (DDRT-PCR)


text: Department of Primary Industries and Fisheries

text: live

text: Generic

text: Differentially amplified transcripts were sub-cloned into TOPO TA-cloning vectors (ONE SHOT chemically competent cells) following the manufacturer's instructions (Invitrogen Corp., CA, USA). Individual E. coli were grown in 5 ml LB ampicillin broths for 18 hrs prior to plasmid extraction from 4 ml using the QIAprep Spin miniprep kit (QIAGEN, MD, USA) and glycerol storage of the remaining 1 ml of broth culture (-80oC). Direct sequencing of plasmid inserts using M13 and T7 primers was undertaken using the BigDye Vers 3.1 technology.

text: Apprximate Day 17 semi-engorged adult female ticks

text: Rhipicephalus microplus

text: N strain larvae (Stewart et al., 1982)

text: whole

text: Feeding adult tick females (F1)

text: E. coli

text: pCR2.1-TOPO and SSH adaptor sequence (Clontech, CA, USA)


text: live

text: Generic

text: Approx. Day 17 adult females collected and placed into mesh bag attached to the bovine neck for 24 hrs prior to harvesting and RNA extraction ('frustrated' females). Differentially amplified transcripts were sub-cloned into TOPO TA-cloning vectors (ONE SHOT chemically competent cells) following the manufacturer's instructions (Invitrogen Corp., CA, USA). Individual E. coli were grown in 5 ml LB ampicillin broths for 18 hrs prior to plasmid extraction from 4 ml using the QIAprep Spin miniprep kit (QIAGEN, MD, USA) and glycerol storage of the remaining 1 ml of broth culture (-80oC). Direct sequencing of plasmid inserts using M13 and T7 primers was undertaken using the BigDye Vers 3.1 technology.

text: Approx. Day 17 adult females collected and placed into mesh bag attached to the bovine neck for 24 hrs ('frustrated' females)

text: Rhipicephalus microplus

text: female

characteristic: sex

text: N strain larvae (Stewart et al., 1982)

text: whole

text: Frustrated Adult Female Tick (F2)

text: E. coli


characteristic: External Id

text: live

text: Generic

text: Larvae post feeding 24 hrs

text: Rhipicephalus microplus

text: N strain larvae (Stewart et al., 1982)

text: whole

text: Feeding tick larvae (L2)

text: E. coli


text: live

text: Generic

text: Larvae collected and placed into mesh bag attached to bovine neck for 24 hrs prior to harvesting and RNA extraction ('frustrated' larvae). Differentially amplified transcripts were sub-cloned into TOPO TA-cloning vectors (ONE SHOT chemically competent cells) following the manufacturer's instructions (Invitrogen Corp., CA, USA). Individual E. coli were grown in 5 ml LB ampicillin broths for 18 hrs prior to plasmid extraction from 4 ml using the QIAprep Spin miniprep kit (QIAGEN, MD, USA) and glycerol storage of the remaining 1 ml of broth culture (-80oC). Direct sequencing of plasmid inserts using M13 and T7 primers was undertaken using the BigDye Vers 3.1 technology.

text: Larvae collected and placed into mesh bag attached to bovine neck for 24 hrs ('frustrated' larvae).

text: Rhipicephalus microplus

text: N strain larvae (Stewart et al., 1982)

text: whole

text: Frustrated tick larvae (L3)

text: E. coli


text: live

text: Generic

text: Adult male ticks (collected from the underside of feeding females)

text: Rhipicephalus microplus

text: N strain larvae (Stewart et al., 1982)

text: whole

text: Adult tick males (M1)

text: E. coli


text: Bowdoin College

text: live

text: Generic

text: neural

characteristic: cell type

text: antepenultimate instar

text: Gryllus bimaculatus

text: prothoracic ganglia

text: Cricket antepenultimate instar 3-days post dennervation Library


text: Clemson University

characteristic: INSDC center name

text: live

text: Generic

text: Total RNA was isolated from apical bud tips from peach. After DNase I treatment (Invitrogen, Carlsbad, CA, USA), polyA+ RNA was purified using Dynabeads Oligo(dT)25 (Invitrogen). The cDNA was synthetised and the suppression subtractive hybridization (SSH) between WT and evg samples at LD and one, two, four and eight weeks of SD was performed following the manufacturer's protocol (Clontech Laboratories, Palo Alto, CA, USA). PCR-amplified products were cloned into pGEM-T easy vector (Promega, Madison, WI, USA). 5,760 clones from subtracted cDNA libraries were screened by hybridization with forward- and reverse-subtracted radiolabeled cDNA. Selected clones from all time points and directions were sequenced using pGEM-T easy vector primer.

text: current year's growth

text: E. coli DH5alpha

text: Prunus persica

text: monoecious

text: apical bud tip

text: Differentially expressed cDNAs between wild-type and evergrowing Prunus persica from long-days to short-days transition

text: plasmid

text: pGEM-T easy


text: University of California at Irvine

characteristic: INSDC first public

text: live

text: Generic

text: mRNA from ca. 200-300 embryos (same as 'B.anynana_embryo_2-10kb' library) Creator SMART cDNA Library Construction Kit (Clontech) 20x amplification of cDNA made from polyA RNA Sequenced by the Joint Genome Institute (code: CBTY)

text: embryos at 0 to 4 days after egg laying

text: Bicyclus anynana

text: mixed (female and, male individuals)

text: 'wildtype' lab stock

text: plasmid

text: pDNR-LIB


text: University of California at Irvine

characteristic: INSDC last update

text: live

text: Generic

text: mRNA from ca. 200-300 embryos (same as 'B.anynana_embryo_nosize' library) Creator SMART cDNA Library Construction Kit (Clontech) 10x amplification of cDNA made from polyA RNA, followed by agarose gel-based size selection [2-10 kb], followed by 15x amplification of sized cDNA Sequenced by the Joint Genome Institute (code: CBTZ)

text: embryos at 0 to 4 days after egg laying

text: Bicyclus anynana

text: mixed (female and, male individuals)

text: 'wildtype' lab stock

text: plasmid

text: pDNR-LIB


text: University of California at Irvine

text: live

characteristic: INSDC status

text: Generic

text: mRNA from 20 individuals Creator SMART cDNA Library Construction Kit (Clontech) 20x amplification of cDNA made from polyA RNA Sequenced by the Joint Genome Institute (code: CBUA)

text: adult

text: Bicyclus anynana

text: mixed (female and, male individuals)

text: 'wildtype' lab stock

text: head

text: plasmid

text: pDNR-LIB


text: University of California at Irvine

text: live

text: Generic

characteristic: NCBI submission model

text: mRNA from 40 individuals (same as 'B.anynana_abdomen_2-10kb' library) Creator SMART cDNA Library Construction Kit (Clontech) 20x amplification of cDNA made from polyA RNA Sequenced by the Joint Genome Institute (code: CBUC)

text: mixed (adults and pupae)

text: Bicyclus anynana

text: mixed (female and, male individuals)

text: 'wildtype' lab stock

text: abdomen

text: plasmid

text: pDNR-LIB


text: University of California at Irvine

text: live

text: Generic

characteristic: NCBI submission package

text: mRNA from 40 individuals (same as 'B.anynana_abdomen_noSize' library) Creator SMART cDNA Library Construction Kit (Clontech) 10x amplification of cDNA made from polyA RNA, followed by agarose gel-based size selection [2-10 kb], followed by 15x amplification of sized cDNA Sequenced by the Joint Genome Institute (code: CBUF)

text: mixed (adults and pupae)

text: Bicyclus anynana

text: mixed (female and, male individuals)

text: 'wildtype' lab stock

text: abdomen

text: plasmid

text: pDNR-LIB


text: University of California at Irvine

text: live

text: Generic

text: mRNA from 39 individuals (same as 'B.anynana_wing.LCPP_2-10kb' library) Creator SMART cDNA Library Construction Kit (Clontech) 20x amplification of cDNA made from polyA RNA Sequenced by the Joint Genome Institute (code: CBUG)

characteristic: description

text: developing wings from fifth instar larvae, crawlers and pre-pupae

text: Bicyclus anynana

text: mixed (female and, male individuals)

text: 'wildtype' lab stock

text: wing

text: plasmid

text: pDNR-LIB


text: University of California at Irvine

text: live

text: Generic

text: mRNA from 39 individuals (same as 'B.anynana_wing.LCPP_nosize' library) Creator SMART cDNA Library Construction Kit (Clontech) 10x amplification of cDNA made from polyA RNA, followed by agarose gel-based size selection [2-10 kb], followed by 15x amplification of sized cDNA Sequenced by the Joint Genome Institute (code: CBUH)

characteristic: dev-stage

text: Bicyclus anynana

text: mixed (female and, male individuals)

text: 'wildtype' lab stock

text: wing

text: plasmid

text: pDNR-LIB


text: University of California at Irvine

text: live

text: Generic

text: mRNA from 35 individuals (same as 'B.anynana_wing.0-3d_2-10kb' library) Creator SMART cDNA Library Construction Kit (Clontech) 20x amplification of cDNA made from polyA RNA Sequenced by the Joint Genome Institute (code: CBUI)

text: wings from pupae at 0 to 3 days after pupation

text: Bicyclus anynana

text: mixed (female and, male individuals)

text: 'wildtype' lab stock

characteristic: strain

text: wing

text: plasmid

text: pDNR-LIB


text: University of California at Irvine

text: live

text: Generic

text: mRNA from 35 individuals (same as 'B.anynana_wing.0-3d_nosize' library) Creator SMART cDNA Library Construction Kit (Clontech) 10x amplification of cDNA made from polyA RNA, followed by agarose gel-based size selection [2-10 kb], followed by 15x amplification of sized cDNA Sequenced by the Joint Genome Institute (code: CBUN)

text: Bicyclus anynana

text: mixed (female and, male individuals)

text: 'wildtype' lab stock

text: wing

text: plasmid

text: pDNR-LIB


text: live

text: Generic

text: mRNA from 24 individuals (same as 'B.anynana_wing.4-6d_2-10kb' library) Creator SMART cDNA Library Construction Kit (Clontech) 20x amplification of cDNA made from polyA RNA Sequenced by the Joint Genome Institute (code: CBUO)

text: wings from pupae at 4 to 6 days after pupation

text: Bicyclus anynana

text: mixed (female and, male individuals)

text: 'wildtype' lab stock

text: wing

text: plasmid

text: pDNR-LIB


text: University of California at Irvine

text: live

text: Generic

text: mRNA from 24 individuals (same as 'B.anynana_wing.4-6d_nosize' library) Creator SMART cDNA Library Construction Kit (Clontech) 10x amplification of cDNA made from polyA RNA, followed by agarose gel-based size selection [2-10 kb], followed by 15x amplification of sized cDNA Sequenced by the Joint Genome Institute (code: CBUP)

text: Bicyclus anynana

text: mixed (female and, male individuals)

text: 'wildtype' lab stock

text: wing

text: plasmid

text: pDNR-LIB


text: Institute of Molecular and Cell Biology, Tartu University

text: live

text: Generic

text: human pregnancy six gestational weeks

text: placenta

characteristic: organ

text: Homo sapiens

text: trophoblastic tissue

text: Human trophoblastic tissue six gestational week

text: Plasmid


text: Institute of Himalayan Bioresource Technology

text: live

text: Generic

text: Leaf

text: Rheum australe

characteristic: organism

text: Leaf

text: Rheum australe differentially expressed ESTs in response to low-temperature

text: plasmid

text: PCR-TRAP (GenHunter)


text: College of Life Science, Nanjing Normal University

text: live

text: Generic

text: megalops stage

text: Eriocheir sinensis

text: Yangtse River

text: abdomen

characteristic: tissue-type

text: Eriocheir sinensis abdomen megalops stage


text: Central Food Technological Research Institute

text: live

text: Generic

text: Vanilla planifolia

text: in vitro Shoot cultures

text: Vanilla planifolia in vitro shoot cultures

characteristic: title


text: National Institute of Biomedical Innovation

text: live

text: Generic

text: 1st strand cDNA was primed with an oligo(dT) primer [ATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded using specific 5' and 3' primers and amplified by PCR. The PCR cDNA was synthesized product was digested with SfiI and size selection was performed to exclude fragments <1.5kb. The SfiI-digested PCR product was cloned into distinct DraIII sites of pME18S-FL3. XhoI sites just outside the DraIII sites can be used to isolate the cDNA insert. Libraries were constructed by oligo-capping method. Cutoff criteria for the quality value (QV) was 15.

text: 3 years old

characteristic: lab-host

text: Macaca fascicularis

text: DraIII (CACTGTGTG)

text: DraIII (CACTGTGTG)

text: male

text: thymus

text: macaque thymus cDNA library QthA

text: pME18S-FL3 (Acc.No. AB009864)

characteristic: vector


characteristic: External Id

text: College of Life Science and Technology, Xinjiang University

text: live

text: Generic

text: Suppression subtractive hybridization (SSH) library of the halophyte Chenopodium album L under salt stress

text: leaf

text: Chenopodium album

text: SSH library of Chenopodium album under salt stress

text: pBS-T vector


text: Stanford University School of Medicine

text: live

text: Generic

text: mRNA was prepared from a collection of California mussels that had been exposed to a variety of environmental challenges that included heat, cold, hypoxia, emersion, heavy metal, and salinity stress. The cDNA library was constructed using Cap-trapping methodology developed by RIKEN. Cap-trapped 1st strand cDNA was produced and hybridized with several biotinylated RNA drivers including: starting mRNA as the normalizing driver, and run-off transcripts from mini-libraries containing highly expressed genes, and previously sequenced cDNAs as subtracting drivers. cDNA that did not hybridize to the biotinylated driver was isolated, directionally-cloned into lambda FLC-I vector (from RIKEN), and plasmids excised using an in vivo liquid excision method.

text: DH10B (T1 phage resistant)

text: Mytilus californianus

text: GGCCAAATCGGCC

text: GGCCCTTATGGCC

text: Gill, mantle, and adductor muscle

text: SHGC-MUW

text: Plasmid

text: pFLC-I


text: University of Victoria

text: live

text: Generic

text: Tissue contributors: Bob Devlin, Fisheries and Oceans Canada

text: brain, kidney, spleen

text: Salmo salar

text: mixed tissue

text: ssalrgf mixed_tissue full-length Salmo salar cDNA


text: University of Victoria

text: live

text: Generic

text: Tissue contributors: Ben Koop

text: brain, kidney, spleen

text: Sebastes caurinus

text: mixed tissue

text: scauevk mixed_tissue Sebastes caurinus cDNA


text: Kunming University of Science and Technology

text: live

text: Generic

text: Reyuan No.2

characteristic: cultivar

text: The suppression subtractive hybridization library for Stylosanthes guianensis under aluminum (Al) stress

text: exposed to Al stress(300uM) for 0.5,2,4,8,12,24,48 and 72 hours respectively

text: root

text: Stylosanthes guianensis

text: root

text: SSH library for Stylosanthes guianensis under aluminum (Al) stress

text: pGEM-T Easy Vector


text: Cornell University

characteristic: INSDC center name

text: live

text: Generic

text: adult

text: accesory gland

text: Heliconius erato

text: male

characteristic: sex

text: petiverana

text: Heliconius erato male accessory gland

text: plasmid

text: pDNR-lib


text: Cornell University

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: accesory gland

text: Heliconius melpomene

text: Heliconius melpomene accessory gland


text: Biogemma

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: mix of 10, 14, 21 and 28 days after pollination

text: Zea mays

text: endosperm

text: ZEASTAR-H


text: Biogemma

text: live

text: Generic

characteristic: NCBI submission package

text: 6-8 days after pollination

text: Zea mays

text: whole kernel

text: ZEASTAR-E


text: Biogemma

text: live

text: Generic

text: 2 days after pollintation

characteristic: dev-stage

text: Zea mays

text: whole kernel

text: ZEASTAR-A


text: Biogemma

text: live

text: Generic

text: 28 days after pollination

text: Zea mays

text: endosperm

text: ZEASTAR-M


text: Biogemma

text: live

text: Generic

text: mix of 14 and 21 days after pollination

text: Zea mays

text: embryo

text: ZEASTAR-J


text: Biogemma

text: live

text: Generic

text: mix of 10 and 21 days after pollination

text: Zea mays

text: pericarp

text: ZEASTAR-I


text: Biogemma

text: live

text: Generic

text: 12 days after pollination

text: Zea mays

text: whole kernel

text: ZEASTAR-G


text: Biogemma

text: live

text: Generic

text: 12 days after pollination

text: Zea mays

text: whole kernel

text: ZEASTAR-F


text: Biogemma

text: live

text: Generic

text: 3-4 days after pollination

text: Zea mays

text: embryo sac

text: ZEASTAR-B


text: Biogemma

text: live

text: Generic

text: mix of 10 and 21 days after pollination

text: Zea mays

text: pericarp


text: Biogemma

text: live

text: Generic

text: 2 days after pollination

text: Zea mays

text: whole kernel

text: ZEASTAR-D


text: Biogemma

text: live

text: Generic

text: 2 days after germination

text: Zea mays

text: aleurone

text: ZEASTAR-N


text: Biogemma

text: live

text: Generic

text: 10 days after pollination

text: Zea mays

text: pericarp


text: Biogemma

text: live

text: Generic

text: 3-4 days after pollination

text: Zea mays

text: surrounding maternal tissue

text: ZEASTAR-C


text: Biogemma

text: live

text: Generic

text: 28 days after pollination

text: Zea mays

text: aleurone

text: ZEASTAR-L


text: University of Tsukuba

text: live

text: Generic

text: mating_type: mat A

characteristic: description

text: Sexual

text: Dictyostelium discoideum

characteristic: strain

text: gamete

text: Dictyostelium discoideum FC (H.Urushihara)


text: University of Tsukuba

text: live

text: Generic

text: gamete

characteristic: cell type

text: mating_type: mat A

text: sexually mature

text: Dictyostelium discoideum

characteristic: organism

text: Dictyostelium discoideum FCL (H.Urushihara)


text: National Institute of Radiological Sciences

text: live

text: Generic

text: mating_type: h minus. The cDNA library of Schizosaccharomyces pombe was prepared by cloning cDNA into the SmaI site of M13mp19 DNA and the direction of DNA sequences was not lways from 5' to 3'. The cDNA data of Schizosaccharomyces pombe are available for searching on the World Wide Web. (URL, http://www.nirs.go.jp)

text: Schizosaccharomyces pombe

text: Schizosaccharomyces pombe late log phase cDNA

characteristic: title


text: National Institute of Genetics

text: live

text: Generic

text: mating_type: mat A

text: Aggregation stage

text: Dictyostelium discoideum

text: Dictyostelium discoideum cDNA library, AF


text: National Institute of Genetics

text: live

text: Generic

text: mating_type: mat A

text: Culmination stage

text: Dictyostelium discoideum

text: Dictyostelium discoideum cDNA library, CF


text: National Institute of Genetics

text: live

text: Generic

text: mating_type: mat A

text: Slug stage

text: Dictyostelium discoideum

text: Dictyostelium discoideum cDNA library, SF


text: National Institute of Genetics

text: live

text: Generic

text: mating_type: mat A

text: Growth phase

text: Dictyostelium discoideum

text: Dictyostelium discoideum cDNA library, VF


text: University of Tsukuba

text: live

text: Generic

text: mating_type: mat A

text: vegetative

text: Dictyostelium discoideum

text: VS


text: University of Tsukuba

text: live

text: Generic

text: mating_type: mat A

text: Sexual

text: Dictyostelium discoideum

text: Gamete

characteristic: tissue-type

text: FC-IC


text: University of Victoria

text: live

text: Generic

text: white blood cell

text: Tissue contributors: Motoshige Yasuike, Ryosuke Yazawa, Kris von Schalburg

text: Oncorhynchus mykiss

text: omykevo WBC Oncorhynchus mykiss cDNA

characteristic: vector


characteristic: External Id

text: University of Victoria

text: live

text: Generic

text: red blood cell

text: Oncorhynchus mykiss

text: omykevn RBC Oncorhynchus mykiss cDNA


text: Universidade de Ribeirao Preto

text: live

text: Generic

text: adult

text: venom gland

characteristic: organ

text: Parawixia bistriata

text: EcoR I

text: Not I

text: female

text: Spider venom gland cDNA

text: plasmid


text: University of Washington

characteristic: INSDC center name

text: live

text: Generic

text: Poly A RNA was prepared from total RNA prep. Full-size, double-stranded cDNA was prepared by SMART synthesis and normalized using kamchatka crab duplex-specific nuclease. SfiI-digested cDNA was cloned in SfiIA and SfiIB sites in pAL17.3 plasmid vector. Sequencing was carried out on a template consisting of vector DNA from cDNA-carrying clones selected by blue/white screeing.

text: cerebellum

text: Papio anubis

text: female

text: plasmid


text: National Institute of Nutrition and Seafood Research

text: live

text: Generic

text: Sequenced EST from Atlantic cod

text: Adult

text: Gadus morhua

text: Liver

text: Atlantic cod adult liver


text: Rubber Research Institute of India

text: live

text: Generic

text: clone RRII105

characteristic: cultivar

text: cDNAs prepared from senescenced leaf tissues of rubber tree

text: rubber tree 18 year old

text: Hevea brasiliensis

text: Leaf tissue

text: senescenced leaf tissues of rubber tree

text: pGEM-T vector


text: Rubber Research Institute of India

characteristic: INSDC first public

text: live

text: Generic

text: clone RRII105

text: cDNAs prepared from leaf tissues of rubber seedlings grown in growth chamber

text: one year old seedlings

text: Hevea brasiliensis

text: Leaf tissue

text: TOPO pCR-4 T vector


text: NOAA National Ocean Service CCEHBR

characteristic: INSDC last update

text: live

text: Generic

text: Normalized library prepared from developmental stages including planula, presettlement polyps, and adult Porites astroides, contain zooxanthellae.

text: planula, free polyp, adult mixture

text: E coli

characteristic: lab-host

text: Porites astreoides

text: Not I

text: Eco RV

text: plasmid


text: RIKEN Tsukuba Institute

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Nourin No.2

text: The soybean full-length cDNA library was constructed essentially as reported previously (Seki et al. (1998) Plant J. 15:707-720; Seki et al. (2002) Science 296:141-145). This clone is in a modified pBluescript vector. This library was constructed from several development stages and tissues. The development stage and tissue of each entry are unknown.

text: Glycine max

text: full-length enriched soybean cDNA library, mixture of seedling, young plant, adult plant


text: National Tsing Hua University

text: live

text: Generic

characteristic: NCBI submission package

text: variety: VC1973A

text: 3 days after imbibition

characteristic: dev-stage

text: Vigna radiata

text: whole seedlings

text: Vigna radiata whole seedlings 3 days after imbibition


text: Texas A&M University

text: live

text: Generic

text: Poly(A) mRNA was purified from the Mauriceville-1c mat A strain (FGSC 2225). Two cultures were grown in Westergaard's medium (synthetic cross medium) for 48 h at 25 degrees C, one with orbital shaking at 200 rpm, and one without shaking. First-strand cDNA containing methyl-C was synthesized from each using an oligo-dT primer bearing a XhoI site to enable directional cloning. After second-strand synthesis, addition of EcoRI adapters, digestion with XhoI, and appropriate work-up, cDNA was ligated to XhoI/EcoRI-digested UNIZAP XR Lambda arms and the ligation products packaged into Lambda particles. The Lambda cDNA library was amplified. After the mass-excision of the amplified libraries equal titers of two phagemid libraries were combined to produce pBluescript phagemid for cDNA sequencing.

characteristic: description

text: Neurospora crassa

text: XhoI

text: EcoRI

text: Mauriceville

characteristic: strain

text: Neurospora crassa cDNA - 48 hours Unfertilized Growth in Crossing Medium

text: Plasmid

text: pBluescriptSK-


text: Texas A&M University

text: live

text: Generic

text: Poly(A) mRNA was purified from the Mauriceville-1c mat A strain (FGSC 2225). Four separate cultures were incubated in 1X Vogel's/1% sorbose/0.1% sucrose for 9 h at 30 degrees C with orbital shaking at 200 rpm, and then to each was added (final concentrations indicated) either (1) 10 mM hydrogen peroxide (2) 750 micromolar sodium arsenite (3) 2.0 mM dithiothreitol and (4) 50 micromolar cadmium chloride and incubation was continued for 1 h. First-strand cDNA containing methyl-C was synthesized using an oligo-dT primer bearing a XhoI site to enable directional cloning. After second-strand synthesis, addition of EcoRI adapters, digestion with XhoI, and appropriate work-up, cDNA was ligated to XhoI/EcoRI-digested UNIZAP XR Lambda arms and the ligation products packaged into Lambda particles. The Lambda cDNA library was amplified. After the mass-excision of the amplified libraries equal titers of the four phagemid libraries were combined to produce pBluescript phagemid for cDNA sequencing.

text: Neurospora crassa

characteristic: organism

text: XhoI

text: EcoRI

text: Mauriceville

text: Neurospora crassa cDNA - 1 hour Oxidative Stress After 9 hours Vegetative Growth

text: Plasmid

text: pBluescriptSK-


text: Texas A&M University

text: live

text: Generic

text: Poly(A) mRNA was purified from a 7 day crossing-culture of Mauriceville-1c mat A (FGSC 2225)crossed with ORS mat a (FGSC 2490). Cells were grown in Westergaard's medium for 5 days prior to initiating crossing. First-strand cDNA containing methyl-C was synthesized using an oligo-dT primer bearing a XhoI site to enable directional cloning. After second-strand synthesis, addition of EcoRI adapters, digestion with XhoI, and appropriate work-up, cDNA was ligated to XhoI/EcoRI-digested UNIZAP XR Lambda arms and the ligation products packaged into Lambda particles. The Lambda cDNA library was amplified. Mass-excision of the amplified library was accomplished to produce pBluescript phagemid for cDNA sequencing.

text: Neurospora crassa

text: XhoI

text: EcoRI

text: A

characteristic: sex

text: Mauriceville-1c mat A (FGSC 2225) and ORS mat a (FGSC 2490)

text: Neurospora crassa cDNA - 7 Days Post-Cross Sexual Growth

characteristic: title

text: Plasmid

text: pBluescriptSK-


text: Texas A&M University

text: live

text: Generic

text: Poly(A) mRNA was purified from the Mauriceville-1c mat A strain (FGSC 2225). Cells were grown in 1X Vogel's medium/2% sucrose for 9 h at 30 degrees C with orbital shaking at 200 rpm; sodium chloride was added to a final concentration of 0.68M and incubation continued for 1 h. First-strand cDNA containing methyl-C was synthesized using an oligo-dT primer bearing a XhoI site to enable directional cloning. After second-strand synthesis, addition of EcoRI adapters, digestion with XhoI, and appropriate work-up, cDNA was ligated to XhoI/EcoRI-digested UNIZAP XR Lambda arms and the ligation products packaged into Lambda particles. The Lambda cDNA library was amplified. Mass-excision of the amplified library was accomplished to produce pBluescript phagemid for cDNA sequencing.

text: Neurospora crassa

text: XhoI

text: EcoRI

text: A

text: Mauriceville FGSC 2225 (Mc1 A)

text: Neurospora crassa cDNA - 1 hour Osmotic Stress After 9 hours Vegetative Growth

text: Plasmid

text: pBluescriptSK-

characteristic: vector


characteristic: External Id

text: Texas A&M University

text: live

text: Generic

text: Poly(A) mRNA was purified from the Mauriceville-1c mat A strain (FGSC 2225). Cells were grown in 1X Vogel's medium/2% sucrose for 7 h at 34 degrees C with orbital shaking at 125 rpm. First-strand cDNA containing methyl-C was synthesized using an oligo-dT primer bearing a XhoI site to enable directional cloning. After second-strand synthesis, addition of EcoRI adapters, digestion with XhoI, and appropriate work-up, cDNA was ligated to XhoI/EcoRI-digested UNIZAP XR Lambda arms and the ligation products packaged into Lambda particles. The Lambda cDNA library was amplified. Mass-excision of the amplified library was accomplished to produce pBluescript phagemid for cDNA sequencing.

text: Neurospora crassa

text: XhoI

text: EcoRI

text: A

text: Mauriceville FGSC 2225 (Mc1 A)

text: Neurospora crassa cDNA - 7 hours Vegetative Growth

text: Plasmid

text: pBluescriptSK-


text: Texas A&M University

text: live

text: Generic

text: Poly(A) mRNA was purified from the Mauriceville-1c mat A strain (FGSC 2225). Cells were grown in 1X Vogel's medium/2% sucrose for 24 h at 30 degrees C with orbital shaking at 200 rpm. First-strand cDNA containing methyl-C was synthesized using an oligo-dT primer bearing a XhoI site to enable directional cloning. After second-strand synthesis, addition of EcoRI adapters, digestion with XhoI, and appropriate work-up, cDNA was ligated to XhoI/EcoRI-digested UNIZAP XR Lambda arms and the ligation products packaged into Lambda particles. The Lambda cDNA library was amplified. Mass-excision of the amplified library was accomplished to produce pBluescript phagemid for cDNA sequencing.

text: Neurospora crassa

text: XhoI

text: EcoRI

text: A

text: Mauriceville FGSC 2225 (Mc1 A)

text: Neurospora crassa cDNA - 24 hours Vegetative Growth

text: Plasmid

text: pBluescriptSK-


text: Texas A&M University

text: live

text: Generic

text: Poly(A) mRNA was purified from the Mauriceville-1c mat A strain (FGSC 2225). Cells were grown in 1X Vogel's medium/2% sucrose for 9 h at 30 degrees C and then for 1 h at 45 degrees C with orbital shaking at 200 rpm. First-strand cDNA containing methyl-C was synthesized using an oligo-dT primer bearing a XhoI site to enable directional cloning. After second-strand synthesis, addition of EcoRI adapters, digestion with XhoI, and appropriate work-up, cDNA was ligated to XhoI/EcoRI-digested UNIZAP XR Lambda arms and the ligation products packaged into Lambda particles. The Lambda cDNA library was amplified. Mass-excision of the amplified library was accomplished to produce pBluescript phagemid for cDNA sequencing.

text: Neurospora crassa

text: XhoI

text: EcoRI

text: A

text: Mauriceville

text: Neurospora crassa cDNA - 1 hour Heat Shock After 9 hour Vegetative Growth

text: Plasmid

text: pBluescriptSK-


text: Texas A&M University

text: live

text: Generic

text: Poly(A) mRNA was purified from the Mauriceville-1c mat A strain (FGSC 2225). Cells were grown in 1X Vogel's medium/2% glucose for 9 h at 30 degrees C with orbital shaking at 200 rpm, and then for 1 h in 1X Vogel's medium lacking glucose. First-strand cDNA containing methyl-C was synthesized using an oligo-dT primer bearing a XhoI site to enable directional cloning. After second-strand synthesis, addition of EcoRI adapters, digestion with XhoI, and appropriate work-up, cDNA was ligated to XhoI/EcoRI-digested UNIZAP XR Lambda arms and the ligation products packaged into Lambda particles. The Lambda cDNA library was amplified. Mass-excision of the amplified library was accomplished to produce pBluescript phagemid for cDNA sequencing.

text: Neurospora crassa

text: XhoI

text: EcoRI

text: Mauriceville FGSC 2225 (Mc1 A)

text: Neurospora crassa cDNA - 1 hour Glucose Deprivation After 9 hours Vegetative Growth

text: Plasmid

text: pBluescriptSK-


text: Texas A&M University

text: live

text: Generic

text: Poly(A) mRNA was purified from the Mauriceville-1c mat A strain (FGSC 2225). Cells were grown in 1X Vogel's medium/2% sucrose for 9 h at 30 degrees C with orbital shaking at 200 rpm, and then for 1 h in 1X Vogel's medium lacking ammonium nitrate. First-strand cDNA containing methyl-C was synthesized using an oligo-dT primer bearing a XhoI site to enable directional cloning. After second-strand synthesis, addition of EcoRI adapters, digestion with XhoI, and appropriate work-up, cDNA was ligated to XhoI/EcoRI-digested UNIZAP XR Lambda arms and the ligation products packaged into Lambda particles. The Lambda cDNA library was amplified. Mass-excision of the amplified library was accomplished to produce pBluescript phagemid for cDNA sequencing.

text: Neurospora crassa

text: XhoI

text: EcoRI

text: A

text: Mauriceville FGSC 2225 (Mc1 A)

text: Neurospora crassa cDNA - 1 hour Nitrogen Deprivation After 9 hours Vegetative Growth

text: Plasmid

text: pBluescriptSK-


text: Veterinary College, Anand Agricultural University

text: live

text: Generic

text: Epithelial cell

characteristic: cell type

text: Total RNA was isolated from mammary gland of lactating of buffalo. Rapid Amplification of cDNA ENDs was done. PCR product was cloned and sequenced.

text: Lactating stage

text: E. coli DH-5alpha

text: Bubalus bubalis

text: dT

text: dT

text: Female

text: Surti

text: Mammary tissue

characteristic: tissue-type

text: Full length cDNA library, Mammary tissue of lactating female buffalo

text: Plasmid


text: Xiamen University

characteristic: INSDC center name

text: live

text: Generic

text: The SSH library was constructed with the thermotolerant Agaricus bisporus strain 02 under high temperature induction enriching for the thermo-responsive up-regulate genes.

text: 2 weeks post-inoculation

text: Agaricus bisporus

text: mycelium

text: Heat-responsive up-regulated SSH libraries (1F/2F) of Agaricus bisporus strain 02


text: Malaysian Palm Oil Board

characteristic: INSDC first public

text: live

text: Generic

text: Inflorescence F12

text: Elaeis guineensis

text: EcoRI

text: NotI

text: Inflorescence

text: FDA (Oil Palm Flower)


text: Malaysian Palm Oil Board

characteristic: INSDC last update

text: live

text: Generic

text: Elaeis guineensis

text: EcoRI

text: Young etiolated seedlings

text: G (Oil Palm Young Etiolated Seedling)


text: Malaysian Palm Oil Board

text: live

text: Generic

text: Elaeis guineensis

text: EcoRI

text: Young etiolated seedlings

text: GT (Oil Palm Young Etiolated Seedling)

text: pT7T3 18U


text: Malaysian Palm Oil Board

text: live

text: Generic

text: 15 WAA Kernel

text: Elaeis guineensis

text: EcoRI

text: Kernel

text: KT (Oil Palm Kernel)

text: pT7T3 18U


text: Malaysian Palm Oil Board

text: live

text: Generic

text: 20 WAA Mesocarp

text: Elaeis guineensis

text: EcoRI

text: Mesocarp

text: MET (Oil Palm Mesocarp)

text: pT7T3 18U


text: Malaysian Palm Oil Board

text: live

text: Generic

text: 15 WAA Mesocarp

text: Elaeis guineensis

text: EcoRI

text: Mesocarp

text: MT (Oil Palm Mesocarp)

text: pT7T3 18U


text: Malaysian Palm Oil Board

text: live

text: Generic

text: Elaeis guineensis

text: EcoRI

text: NotI

text: Root

text: RD (Oil Palm Root)


text: Malaysian Palm Oil Board

text: live

text: Generic

text: Inflorescence F19

text: Elaeis guineensis

text: EcoRI

text: Inflorescence

text: SFB (Oil Palm Inflorescence substracted with Leaf)

text: pT7T3 18U


text: National Institute of Allergy and Infectious Diseases

text: live

text: Generic

text: Salivary gland

text: PCR-based cDNA library made with SMART cDNA library construction kit from Clontech

text: Adult

text: Salivary gland

characteristic: organ

text: Phlebotomus arabicus

text: Female

text: Israel

text: Salivary gland

text: Adult female Phlebotomus arabicus salivary gland library

text: Phage

text: Lambda TriplEx2


text: WageningenUR-Plant Breeding

text: live

characteristic: INSDC status

text: Generic

text: ACCESSION: CGN12041; Sinde. First strand cDNA 1 ug of total RNA was treated with DNAse I (Invitrogen, amplification grade; 18068-015) followed by RT PCR (Invitrogen SuperScriptTM III First-Strand Synthesis System for RT-PCR; 18080-051) using random hexamer primers in a final reaction volume of 20 ul. Samples without SuperScriptTM III reverse transcriptase (minus RT PCR) were included as controls for the DNAse I treatment. A fraction of 2 ul of the cDNA was used as a template in a PCR reaction with alpha-gliadin specific primers (alpha1F: 5'atg aaR acM ttt cYc atc-3';alpha5R; 5'gtt agt acc gaa gat gcc-3'). Subsequently, the PCR products were cloned in pGEM-T Easy (Promega).

text: 14 days post-anthesis

text: single plant from CGN12041

characteristic: isolate

text: E-coli; XL1-blue

characteristic: lab-host

text: Triticum turgidum subsp. durum

text: durum

characteristic: sub species

text: developing seed

text: Sinde wheat Developing seeds, 14 days post-anthesis

text: plasmid

text: pGEMt easy


text: WageningenUR-Plant Breeding

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: ACCESSION: CGN12287; 'Tripshiro'. First strand cDNA 1 ug of total RNA was treated with DNAse I (Invitrogen, amplification grade; 18068-015) followed by RT PCR (Invitrogen SuperScriptTM III First-Strand Synthesis System for RT-PCR; 18080-051) using random hexamer primers in a final reaction volume of 20 ul. Samples without SuperScriptTM III reverse transcriptase (minus RT PCR) were included as controls for the DNAse I treatment. A fraction of 2 ul of the cDNA was used as a template in a PCR reaction with alpha-gliadin specific primers (alpha1F: 5'atg aaR acM ttt cYc atc-3';alpha5R; 5'gtt agt acc gaa gat gcc-3'). Subsequently, the PCR products were cloned in pGEM-T Easy (Promega).

characteristic: description

text: 14 days post-anthesis

characteristic: dev-stage

text: single plant from CGN12287

text: E-coli; XL1-blue

text: Triticum turgidum subsp. durum

text: durum

text: developing seed

text: Tripshiro wheat Developing seeds, 14 days post-anthesis

text: plasmid

text: pGEMt easy


text: Khon Kaen University

text: live

text: Generic

text: hepatocyte and cholangiocyte

text: infected hamster with opisthorchiasis on day 30

text: Liver

text: Mesocricetus auratus

characteristic: organism

text: Male

characteristic: sex

text: Syrian

characteristic: strain

text: Liver

text: Mesocricetus auratus hepatocyte and cholangiocyte

characteristic: title


characteristic: External Id

text: Genoscope - Centre National de Sequencage

text: live

text: Generic

text: genotype: Scavina6

text: Theobroma cacao

text: Leave

characteristic: tissue-type


characteristic: INSDC center name

characteristic: INSDC first public

text: live

text: Generic

text: genotype: Scavina6; flower_self pollinated by SIC23

text: Theobroma cacao

text: Flower


characteristic: INSDC last update

text: live

text: Generic

text: genotype: Scavina6; flower_self pollinated

text: Theobroma cacao

text: Flower


text: The Kansai Electric Power Co.

text: live

characteristic: INSDC status

text: Generic

text: Chlamydomonas sp. HS-5

text: Chlamydomonas sp. HS-5 lambda ZAP cDNA library


text: Western Kentucky University

text: live

text: Generic

text: Helianthus annuus

text: Shoots and roots

text: Helianthus annuus suppression subtractive cDNA library


text: University of Utah

text: live

text: Generic

text: The cDNA library was constructed using CloneMiner cDNA library construction kit from Invitrogen

text: Adhesive gland

characteristic: organ

text: Phragmatopoma californica

text: Phragmatopoma Californica adhesive gland cDNA library


text: Michigan State University

text: live

text: Generic

text: 22 days post-anthesis

text: Tropaeolum majus

text: cotyledon

text: Nasturtium 22 days post-anthesis library


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT)-primed cDNA and cloned into the pMCL200_cDNA vector. PolyA RNA was primed with oligo(dT) primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'-CGGACGCGTGGG) and digested with NotI. cDNA was size selected >2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pMCL200_cDNA vector. Library was created and sequencing done by the DOE Joint Genome Institute (Walnut Creek, CA).

text: trophozoite

text: Spironucleus vortens

text: SalI

text: NotI

text: CAPO Spironucleus vortens trophozoite stage

characteristic: vector


text: DOE Joint Genome Institute

text: live

text: Generic

text: The library was made from oligo(dT)-primed cDNA and cloned into the pMCL200_cDNA vector. PolyA RNA was primed with oligo(dT) primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'-CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 1-2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pMCL200_cDNA vector. Library was created and sequencing done by the DOE Joint Genome Institute (Walnut Creek, CA).

text: trophozoite

text: Spironucleus vortens

text: SalI

text: NotI

text: CAPP Spironucleus vortens trophozoite stage


text: Departamento de Biologia, Universidade de Aveiro

text: live

text: Generic

text: Subtracted cDNA library

text: Liver

text: Dicentrarchus labrax

text: Dicentrarchus labrax liver, forward subtracted cDNA library

text: pGEM-T Easy (Promega)


text: live

text: Generic

text: Subtracted cDNA library

text: Liver

text: Dicentrarchus labrax

text: Dicentrarchus labrax liver, reverse subtracted cDNA library

text: pGEM-T Easy (Promega)


text: Yamaguchi University

text: live

text: Generic

text: host = Brassica rapa subsp. pekinensis; mRNA expressed in the host plant

text: secondary plasmodia

text: Hagi

characteristic: isolate

text: Plasmodiophora brassicae

text: Plasmodiophora brassicae secondary plasmodia


text: Yunnan Agricultural University

text: live

text: Generic

characteristic: NCBI submission model

text: breed wujin.

text: Sus scrofa

text: longissimus dorsi muscle

text: wujin pig muscle full-length cDNA library

text: pDNR-LIB


text: Station Biologique

text: live

text: Generic

characteristic: NCBI submission package

text: adults

text: Bathymodiolus thermophilus

text: gill, adductor muscle, mantle and foot

text: subtracted libraries from Bathymodiolus thermophilus exposed to thermal stress


text: Station Biologique

text: live

text: Generic

text: Paralvinella pandorae exposed to thermal stress

characteristic: description

text: adults

text: Nautalvinella pandorae

text: whole individual

text: subtracted libraries from paralvinella exposed to temperature challenge


text: M.S. Swaminathan Research Foundation

text: live

text: Generic

text: A fresh fully matured flower from a male Pandanus fascicularis plant was sampled and total RNA isolated by a Lithium chloride based cold extraction buffer procedure. Ist and IInd strand cDNA was synthesised according to the manufacturer's instructions (Creator SMART cDNA library construction kit, BD Biosciences). The cDNA was then size-fractionated and directionally cloned between the 5' Sfi IA and 3' Sfi IB sites of pDNR_LIB vector. Single-pass sequencing from the 5' end of the ESTs was performed using M13 forward primer in a automated sequencer (ABI 3130, Applied Biosystems). All the sequenced ESTs were screened for vector backbone contamination using the Vecscreen function. Their putative function was determined by performing the TBLASTX (nr/nt, nucleotide collection) and BLASTX algorithms using their default parameters.

text: Fully matured

characteristic: dev-stage

text: Flower

text: Pandanus odoratissimus

text: Sfi I A

text: Sfi I B

text: Male

text: Wild

text: Flower

text: Pandanus fascicularis L. flower-specific cDNA library


text: Sichuan Agriculture University

text: live

text: Generic

text: inbred line Bt-1

characteristic: cultivar

text: Sequence was from the Fusarium moniliforme-infection differentially resistant genes in maize (Zea mays L.) using suppression subtractive hybridization. Expressed sequence tags from the Reverse SSH library in Bt-1 inbred line.

text: Zea mays

characteristic: organism

text: Reverse SSH library in Bt-1 inbred line


text: Sichuan Agriculture University

text: live

text: Generic

text: inbred line Bt-1

text: Sequence was from the Fusarium moniliforme-infection differentially resistant genes in maize (Zea mays L.) using suppression subtractive hybridization. Expressed sequence tags from the Forward SSH library in Bt-1 inbred line.

text: Zea mays

text: Forward SSH library in Bt-1 inbred line


text: Sichuan Agriculture University

text: live

text: Generic

text: inbred line Ye478

text: Sequence was from the Fusarium moniliforme-infection differentially resistant genes in maize (Zea mays L.) using suppression subtractive hybridization. Expressed sequence tags from the Forward SSH library in Ye478 inbred line.

text: Zea mays


text: Sichuan Agriculture University

text: live

text: Generic

text: inbred line Ye478

text: Sequence was from the Fusarium moniliforme-infection differentially resistant genes in maize (Zea mays L.) using suppression subtractive hybridization. Expressed sequence tags from the Reverse SSH library in Ye478 inbred line.

text: Zea mays


text: University of Helsinki

text: live

text: Generic

text: 'Baron Solemacher'

text: TOP-10 (Invitrogen)

characteristic: lab-host

text: Fragaria vesca

text: Shoot apex

text: Suppression subtractive hybridization library EB

text: pGEM-T Easy (Promega)


text: University of Helsinki

text: live

text: Generic

text: TOP-10 (Invitrogen)

text: Fragaria vesca

text: Shoot apex

text: Suppression subtractive hybridization library SD

characteristic: title

text: pGEM-T Easy (Promega)


characteristic: External Id

text: National Institute for Plant Genome Research

text: live

text: Generic

text: Pusa Ruby

text: Subtraction library cloned in pGEM-T Easy Vector

text: Solanum lycopersicum

text: Seedling

text: Geraniol treated Tomato seedling subtraction library


text: University of California

text: live

text: Generic

text: Morex

text: Seeds were surface sterilized then germinated under axenic conditions in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Five-day old seedlings were incubated at 5 degrees C for 2 days. Shoots were then harvested, total RNA was prepared, poly(A) RNA was purified, one primary unamplified cDNA library was made using lambdaZAP, and 600000 pfu were in vivo excised to yield pBluescript SK(-) cDNA phagemids. These steps were performed in the TJ Close laboratory at the University of California, Riverside (Choi, Close, Fenton). Phagemids were plated and picked at the Clemson University Genomics Institute (CUGI) (Begum, Palmer, Frisch, Atkins, Wing). Plasmid DNA preparations and DNA sequencing were performed at CUGI (Wing, Yu, Frisch, Henry, Simmons, Oates, Rambo). The sequences were transmitted from CUGI (Main) to UCR (Close, Wanamaker) then trimmed to remove vector sequence and to a minimum of 100 bases of phred 17 or above. For more details on library preparation and sequencing see http://www.genome.clemson.edu/projects/barley. To order this clone see http://www.genome.clemson.edu/orders. Also see Close TJ, Wing R, Kleinhofs A, Wise R (2001) Genetically and physically anchored EST resources for barley genomics. Barley Genetics Newsletter 31:29-30 (http://wheat.pw.usda.gov/ggpages/bgn/31/cover.html).

text: seedling

text: E. coli TJC121

text: Hordeum vulgare subsp. vulgare

text: EcoRI

text: XhoI

text: seedling shoot

characteristic: tissue-type

text: Hordeum vulgare seedling shoot (cold stress) cDNA library HVSMEa (HVcDNA0001)2

text: Plasmid

text: pBluescript SK(-)


text: University of California

characteristic: INSDC center name

text: live

text: Generic

text: Morex

text: Seeds were surface sterilized then germinated under axenic conditions in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Five-day old seedlings were incubated at 90% RH for 24 hr. Shoots were then harvested, total RNA was prepared, poly(A) RNA was purified, one primary unamplified cDNA library was made using lambdaZAP, and 600000 pfu were in vivo excised to yield pBluescript SK(-) cDNA phagemids. These steps were performed in the TJ Close laboratory at the University of California, Riverside (Choi, Close, Fenton). Phagemids were plated and picked at the Clemson University Genomics Institute (CUGI) (Begum, Palmer, Frisch, Atkins, Wing). Plasmid DNA preparations and DNA sequencing were performed at CUGI (Wing, Yu, Frisch, Henry, Simmons, Oates, Rambo). The sequences were transmitted from CUGI (Main) to UCR (Close, Wanamaker) then trimmed to remove vector sequence and to a minimum of 100 bases of phred 17 or above. For more details on library preparation and sequencing see http://www.genome.clemson.edu/projects/barley. To order this clone see http://www.genome.clemson.edu/orders. Also see Close TJ, Wing R, Kleinhofs A, Wise R (2001) Genetically and physically anchored EST resources for barley genomics. Barley Genetics Newsletter 31:29-30 (http://wheat.pw.usda.gov/ggpages/bgn/31/cover.html).

text: seedling

text: E. coli TJC121

text: Hordeum vulgare subsp. vulgare

text: EcoRI

text: XhoI

text: seedling shoot

text: Hordeum vulgare seedling shoot (dehydration stress) cDNA library HVSMEb (HVcDNA0002)2

text: Plasmid

text: pBluescript SK(-)


text: University of California

characteristic: INSDC first public

text: live

text: Generic

text: Morex

text: Seeds were surface sterilized then germinated under axenic conditions in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Five-day old seedling shoots were then harvested, total RNA was prepared, poly(A) RNA was purified, one primary unamplified cDNA library was made using lambdaZAP, and 1 million pfu were in vivo excised to yield pBluescript SK(-) cDNA phagemids. These steps were performed in the TJ Close laboratory at the University of California, Riverside (Choi, Close, Fenton). Phagemids were plated and picked at the Clemson University Genomics Institute (CUGI) (Begum, Palmer, Frisch, Atkins, Wing). Plasmid DNA preparations and DNA sequencing were performed at CUGI (Wing, Yu, Frisch, Henry, Simmons, Oates, Rambo). The sequences were transmitted from CUGI (Main) to UCR (Close, Wanamaker) then trimmed to remove vector sequence and to a minimum of 100 bases of phred 17 or above. For more details on library preparation and sequencing see http://www.genome.clemson.edu/projects/barley. To order this clone see http://www.genome.clemson.edu/orders. Also see Close TJ, Wing R, Kleinhofs A, Wise R (2001) Genetically and physically anchored EST resources for barley genomics. Barley Genetics Newsletter 31:29-30 (http://wheat.pw.usda.gov/ggpages/bgn/31/cover.html).

text: seedling

text: E. coli TJC121

text: Hordeum vulgare subsp. vulgare

text: EcoRI

text: XhoI

text: seedling shoot

text: Hordeum vulgare seedling shoot (etiolated unstressed) cDNA library HVSMEc (HVcDNA0003)2

text: Plasmid

text: pBluescript SK(-)


text: University of California

characteristic: INSDC last update

text: live

text: Generic

text: Morex

text: Seeds were surface sterilized then germinated under axenic conditions in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Five-day old seedling roots were then harvested, total RNA was prepared, poly(A) RNA was purified, one primary unamplified cDNA library was made using lambdaZAP, and 1 million pfu were in vivo excised to yield pBluescript SK(-) cDNA phagemids. These steps were performed in the TJ Close laboratory at the University of California, Riverside (Choi, Close, Fenton). Phagemids were plated and picked at the Clemson University Genomics Institute (CUGI) (Begum, Palmer, Frisch, Atkins, Wing). Plasmid DNA preparations and DNA sequencing were performed at CUGI (Wing, Yu, Frisch, Henry, Simmons, Oates, Rambo). The sequences were transmitted from CUGI (Main) to UCR (Close, Wanamaker) then trimmed to remove vector sequence and to a minimum of 100 bases of phred 17 or above. For more details on library preparation and sequencing see http://www.genome.clemson.edu/projects/barley. To order this clone see http://www.genome.clemson.edu/orders. Also see Close TJ, Wing R, Kleinhofs A, Wise R (2001) Genetically and physically anchored EST resources for barley genomics. Barley Genetics Newsletter 31:29-30 (http://wheat.pw.usda.gov/ggpages/bgn/31/cover.html).

text: seedling

text: E. coli TJC121

text: Hordeum vulgare subsp. vulgare

text: EcoRI

text: XhoI

text: seedling shoot

text: Hordeum vulgare seedling root (etiolated unstressed) cDNA library HVSMEf (HVcDNA0007)2

text: Plasmid

text: pBluescript SK(-)


text: University of California

text: live

characteristic: INSDC status

text: Generic

text: Morex

text: Plants were grown in the greenhouse at the University of California, Riverside (Fenton, SJ Close, TJ Close). Whole spike with awns trimmed were collected at white, green and yellow anther stages (Fenton). Total RNA was prepared from each pool, equal quantities of all three RNA pools were combined, poly(A) RNA was purified from the mixture, one primary unamplified cDNA library was made using lambdaZAP, and 1 million pfu were excised to yield pBluescript SK(-) cDNA phagemids. These steps were performed in the TJ Close laboratory at the University of California, Riverside (Choi, Close, Fenton). Phagemids were plated and picked at the Clemson University Genomics Institute (CUGI) (Begum, Palmer, Frisch, Atkins, Wing). Plasmid DNA preparations and DNA sequencing were performed at CUGI (Wing, Yu, Frisch, Henry, Simmons, Oates, Rambo). The sequences were transmitted from CUGI (Main) to UCR (Close, Wanamaker) then trimmed to remove vector sequence and to a minimum of 100 bases of phred 17 or above. For more details on library preparation and sequencing see http://www.genome.clemson.edu/projects/barley. To order this clone see http://www.genome.clemson.edu/orders. Also see Close TJ, Wing R, Kleinhofs A, Wise R (2001) Genetically and physically anchored EST resources for barley genomics. Barley Genetics Newsletter 31:29-30 (http://wheat.pw.usda.gov/ggpages/bgn/31/cover.html).

text: reproductive

text: E. coli SOLR

text: Hordeum vulgare subsp. vulgare

text: EcoRI

text: XhoI

text: pre-anthesis spike

text: Hordeum vulgare pre-anthesis spike (white to yellow anther) cDNA library HVSMEg (HVcDNA0008)2

text: Plasmid

text: pBluescript SK(-)


text: University of California

text: live

text: Generic

text: Morex

text: Plants were grown in the greenhouse at the University of California, Riverside (Fenton, SJ Close, TJ Close). Whole spike with awns trimmed were collected at 5, 10, 15, 20, 30 and 45 DAP (Fenton). Total RNA was prepared from each pool, equal quantities of all six RNA pools were combined, poly(A) RNA was purified from the mixture, one primary unamplified cDNA library was made using lambdaZAP, and 1 million pfu were excised to yield pBluescript SK(-) cDNA phagemids. These steps were performed in the TJ Close laboratory at the University of California, Riverside (Choi, Close, Fenton). Phagemids were plated and picked at the Clemson University Genomics Institute (CUGI) (Begum, Palmer, Frisch, Atkins, Wing). Plasmid DNA preparations and DNA sequencing were performed at CUGI (Wing, Yu, Frisch, Henry, Simmons, Oates, Rambo). The sequences were transmitted from CUGI (Main) to UCR (Close, Wanamaker) then trimmed to remove vector sequence and to a minimum of 100 bases of phred 17 or above. For more details on library preparation and sequencing see http://www.genome.clemson.edu/projects/barley. To order this clone see http://www.genome.clemson.edu/orders. Also see Close TJ, Wing R, Kleinhofs A, Wise R (2001) Genetically and physically anchored EST resources for barley genomics. Barley Genetics Newsletter 31:29-30 (http://wheat.pw.usda.gov/ggpages/bgn/31/cover.html).

text: reproductive

text: E. coli SOLR

text: Hordeum vulgare subsp. vulgare

text: EcoRI

text: XhoI

text: 5-45 DAP spike

text: Hordeum vulgare 5-45 DAP spike cDNA library HVSMEh (HVcDNA0009)2

text: Plasmid

text: pBluescript SK(-)


text: University of California

text: live

text: Generic

text: Morex

text: Plants were grown in the greenhouse at the University of California, Riverside (Fenton, SJ Close, TJ Close). Whole spike with awns trimmed were collected at 20 DAP (Fenton). Total RNA was prepared from each pool, equal quantities of all six RNA pools were combined, poly(A) RNA was purified from the mixture, one primary unamplified cDNA library was made using lambdaZAP, and 1 million pfu were excised to yield pBluescript SK(-) cDNA phagemids. These steps were performed in the TJ Close laboratory at the University of California, Riverside (Choi, Close, Fenton). Phagemids were plated and picked at the Clemson University Genomics Institute (CUGI) (Begum, Palmer, Frisch, Atkins, Wing). Plasmid DNA preparations and DNA sequencing were performed at CUGI (Wing, Yu, Frisch, Henry, Simmons, Oates, Rambo). The sequences were transmitted from CUGI (Main) to UCR (Close, Wanamaker) then trimmed to remove vector sequence and to a minimum of 100 bases of phred 17 or above. For more details on library preparation and sequencing see http://www.genome.clemson.edu/projects/barley. To order this clone see http://www.genome.clemson.edu/orders. Also see Close TJ, Wing R, Kleinhofs A, Wise R (2001) Genetically and physically anchored EST resources for barley genomics. Barley Genetics Newsletter 31:29-30 (http://wheat.pw.usda.gov/ggpages/bgn/31/cover.html).

text: reproductive

text: E. coli SOLR

text: Hordeum vulgare subsp. vulgare

text: EcoRI

text: XhoI

text: 20 DAP spike

text: Hordeum vulgare 20 DAP spike cDNA library HVSMEi (HVcDNA0010)2

text: Plasmid

text: pBluescript SK(-)


text: University of California

text: live

text: Generic

text: Morex

text: Plants were raised from seeds in a Controlled Environments growth chamber maintained in continuous light at 18 degrees C, and testa and pericarp were dissected from developing kernels at Washington State University, Pullman, WA (Kannangara, von Wetstein). At the University of California, Riverside (Choi, Close, Fenton), total RNA was prepared, poly(A) RNA was purified, one cDNA library was made using lambda ZAP, and 1 million pfu were excised to yield pBluescript SK(-) cDNA phagemids. Phagemids were plated and picked at the Clemson University Genomics Institute (CUGI) (Begum, Palmer, Frisch, Atkins, Wing). Plasmid DNA preparations and DNA sequencing were performed at CUGI (Wing, Yu, Frisch, Henry, Simmons, Oates, Rambo). The sequences were transmitted from CUGI (Main) to UCR (Close, Wanamaker) then trimmed to remove vector sequence and to a minimum of 100 bases of phred 17 or above. For more details on library preparation and sequencing see http://www.genome.clemson.edu/projects/barley. To order this clone see http://www.genome.clemson.edu/orders. Also see Close TJ, Wing R, Kleinhofs A, Wise R (2001) Genetically and physically anchored EST resources for barley genomics. Barley Genetics Newsletter 31:29-30 (http://wheat.pw.usda.gov/ggpages/bgn/31/cover.html).

text: developing kernels

text: E. coli TJC121

text: Hordeum vulgare subsp. vulgare

text: EcoRI

text: XhoI

text: testa/pericarp

text: Hordeum vulgare testa/pericarp cDNA library HVSMEk (HVcDNA0013)2

text: Plasmid

text: pBluescript SK(-)


text: University of California

text: live

text: Generic

text: Morex

text: Plants were grown at the University of Minnesota in the GJ Muehlbauer lab; spikes were harvested and snap frozen at 0, 1, 2, 3, 4, 5, 6, and 8 days after Fusarium graminearum inoculation (Heinen). At the University of California, Riverside (Choi, Close, Fenton), total RNA was prepared, poly(A) RNA was purified, one cDNA library was made using lambda ZAP, and 1 million pfu were excised to yield pBluescript SK(-) cDNA phagemids. Phagemids were plated and picked at the Clemson University Genomics Institute (CUGI) (Begum, Palmer, Frisch, Atkins, Wing). Plasmid DNA preparations and DNA sequencing were performed at CUGI (Wing, Yu, Frisch, Henry, Simmons, Oates, Rambo). The sequences were transmitted from CUGI (Main) to UCR (Close, Wanamaker) then trimmed to remove vector sequence and to a minimum of 100 bases of phred 17 or above. For more details on library preparation and sequencing see http://www.genome.clemson.edu/projects/barley. To order this clone see http://www.genome.clemson.edu/orders. Also see Close TJ, Wing R, Kleinhofs A, Wise R (2001) Genetically and physically anchored EST resources for barley genomics. Barley Genetics Newsletter 31:29-30 (http://wheat.pw.usda.gov/ggpages/bgn/31/cover.html).

text: reproductive

text: E. coli TJC121

text: Hordeum vulgare subsp. vulgare

text: EcoRI

text: XhoI

text: spike

text: Hordeum vulgare spike (Fusarium infected) cDNA library HVSMEl (HVcDNA0012)2

text: Plasmid

text: pBluescript SK(-)


text: University of California

text: live

text: Generic

text: Morex

text: Morex (mla) plants were greenhouse grown in the R Wise lab at Iowa State University, Ames, IA; 7 day old green seedlings were infected with isolate 5874 of Blumeria graminis f. sp. hordei, and leaves were harvested 24, 48 and 72 hr post-inoculation and snap frozen (Wise). In the TJ Close lab at the University of California, Riverside, total RNA was prepared from each sample pool, equal quantities of all three RNA pools were combined, poly(A) RNA was purified from the mixture, one primary unamplified cDNA library was made, and 1 million pfu were excised to yield pBluescript SK(-) cDNA phagemids. Phagemids were plated and picked at the Clemson University Genomics Institute (CUGI) (Begum, Palmer, Frisch, Atkins, Wing). Plasmid DNA preparations and DNA sequencing were performed at CUGI (Wing, Yu, Frisch, Henry, Simmons, Oates, Rambo). The sequences were transmitted from CUGI (Main) to UCR (Close, Wanamaker) then trimmed to remove vector sequence and to a minimum of 100 bases of phred 17 or above. For more details on library preparation and sequencing see http://www.genome.clemson.edu/projects/barley. To order this clone see http://www.genome.clemson.edu/orders. Also see Close TJ, Wing R, Kleinhofs A, Wise R (2001) Genetically and physically anchored EST resources for barley genomics. Barley Genetics Newsletter 31:29-30 (http://wheat.pw.usda.gov/ggpages/bgn/31/cover.html).

text: seedling

text: E. coli TJC121

text: Hordeum vulgare subsp. vulgare

text: EcoRI

text: XhoI

text: green seedling leaf

text: Hordeum vulgare green seedling (Blumeria infected) cDNA library HVSMEm (HVcDNA0014)2

text: Plasmid

text: pBluescript SK(-)


text: University of California

text: live

text: Generic

text: Morex

text: Plants were grown at Washington State University, Pullman, WA in a greenhouse, the rachises were excised and frozen in liquid nitrogen (Kleinhofs lab). In the TJ Close lab at the University of California, Riverside total RNA was prepared, poly(A) was purified, one primary unamplified cDNA library was made, and 1 million pfu were excised to yield pBluescript SK(-) cDNA phagemids. Phagemids were plated and picked at the Clemson University Genomics Institute (CUGI) (Begum, Palmer, Frisch, Atkins, Wing). Plasmid DNA preparations and DNA sequencing were performed at CUGI (Wing, Yu, Frisch, Henry, Simmons, Oates, Rambo). The sequences were transmitted from CUGI (Main) to UCR (Close, Wanamaker) then trimmed to remove vector sequence and to a minimum of 100 bases of phred 17 or above. For more details on library preparation and sequencing see http://www.genome.clemson.edu/projects/barley. To order this clone see http://www.genome.clemson.edu/orders. Also see Close TJ, Wing R, Kleinhofs A, Wise R (2001) Genetically and physically anchored EST resources for barley genomics. Barley Genetics Newsletter 31:29-30 (http://wheat.pw.usda.gov/ggpages/bgn/31/cover.html).

text: reproductive

text: E. coli TJC121

text: Hordeum vulgare subsp. vulgare

text: EcoRI

text: XhoI

text: rachis

text: Hordeum vulgare rachis cDNA library HVSMEn (HVcDNA0015)2

text: Plasmid

text: pBluescript SK(-)


text: University of California

text: live

text: Generic

text: CI16155 (Mla13)

text: CI16155 (Mla13) plants were greenhouse grown in the R Wise lab at Iowa State University, Ames, IA; 7 day old green seedlings were challenged with isolate A27 (AvrMla13) of Blumeria graminis f. sp. hordei, and leaves were harvested 20 and 24 hr post-inoculation and snap frozen; uninoculated leaves were harvested 20 hr post-inoculation (Wei, Wise). In the TJ Close lab at the University of California, Riverside, total RNA was prepared from each barley sample pool, equal quantities of all three RNA samples were combined, poly(A) RNA was purified from the mixture, one cDNA library was made using lambdaZAP, and 1 million pfu were in vivo excised to yield pBluescript SK(-) cDNA phagemids (Choi, Fenton, Close). Phagemids were plated and picked at the Clemson University Genomics Institute (CUGI) (Begum, Palmer, Frisch, Atkins, Wing). Plasmid DNA preparations and DNA sequencing were performed at CUGI (Wing, Yu, Frisch, Henry, Simmons, Oates, Rambo). The sequences were transmitted from CUGI (Main) to UCR (Close, Wanamaker) then trimmed to remove vector sequence and to a minimum of 100 bases of phred 17 or above. For more details on library preparation and sequencing see http://www.genome.clemson.edu/projects/barley. To order this clone see http://www.genome.clemson.edu/orders. Also see Close TJ, Wing R, Kleinhofs A, Wise R (2001) Genetically and physically anchored EST resources for barley genomics. Barley Genetics Newsletter 31:29-30 (http://wheat.pw.usda.gov/ggpages/bgn/31/cover.html).

text: seedling

text: E. coli TJC121

text: Hordeum vulgare subsp. vulgare

text: EcoRI

text: XhoI

text: seedling green leaf

text: Hordeum vulgare (Blumeria challenged) seedling green leaf cDNA library HV_CEa (HVcDNA0004)2

text: Plasmid

text: pBluescript SK(-)


text: University of California

text: live

text: Generic

text: CI16151 (Mla6)

text: CI16151 (Mla6) plants were greenhouse grown in the R Wise lab at Iowa State University, Ames, IA; 7 day old green seedlings were challenged with isolate 5874 (AvrMla6) of Blumeria graminis f. sp. hordei, and leaves were harvested 20 and 24 hr post-inoculation and snap frozen; uninoculated leaves were harvested 20 hr post-inoculation (Wei, Wise). In the TJ Close lab at the University of California, Riverside, total RNA was prepared from each barley sample pool, equal quantities of all three RNA samples were combined, poly(A) RNA was purified from the mixture, one cDNA library was made using lambdaZAP, and 1 million pfu were in vivo excised to yield pBluescript SK(-) cDNA phagemids (Choi, Fenton, Close). Phagemids were plated and picked at the Clemson University Genomics Institute (CUGI) (Begum, Palmer, Frisch, Atkins, Wing). Plasmid DNA preparations and DNA sequencing were performed at CUGI (Wing, Yu, Frisch, Henry, Simmons, Oates, Rambo). The sequences were transmitted from CUGI (Main) to UCR (Close, Wanamaker) then trimmed to remove vector sequence and to a minimum of 100 bases of phred 17 or above. For more details on library preparation and sequencing see http://www.genome.clemson.edu/projects/barley. To order this clone see http://www.genome.clemson.edu/orders. Also see Close TJ, Wing R, Kleinhofs A, Wise R (2001) Genetically and physically anchored EST resources for barley genomics. Barley Genetics Newsletter 31:29-30 (http://wheat.pw.usda.gov/ggpages/bgn/31/cover.html).

text: seedling

text: E. coli SOLR

text: Hordeum vulgare subsp. vulgare

text: EcoRI

text: XhoI

text: seedling green leaf

text: Hordeum vulgare (Blumeria challenged) seedling green leaf cDNA library HV_CEb (HVcDNA0005)2

text: Plasmid

text: pBluescript SK(-)


text: Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences

text: live

text: Generic

text: mixed tissues from head kidney, liver, intestine and spleen

characteristic: organ

text: Cynoglossus semilaevis

text: Sfi IA

text: Sfi IB

text: CSGMMA: Normalized half sole tongue sole cDNA library from mixed tissues of head kidney, liver, intestine and spleen

text: Phagemid

text: pDNR-Lib


text: live

text: Generic

text: ovary

text: Cynoglossus semilaevis

text: Sfi IA

text: Sfi IB

text: CSGOVB: Normalized half sole tongue sole cDNA library from ovary

text: Phagemid

text: pDNR-Lib

characteristic: vector


text: live

text: Generic

text: testis

text: Cynoglossus semilaevis

text: Sfi IA

text: Sfi IB

text: CSGSPA: Normalized half sole tongue sole cDNA library from testis

text: Phagemid

text: pDNR-Lib


text: Division of Plant Pathology, IARI, New Delhi

text: live

text: Generic

text: Suppressive Subtracted cDNA of resistant wheat cultivar after inoculation with Bipolaris sorokiniana

text: Seedling

text: XL-1 Blue

text: Triticum aestivum

text: Young leaves

text: Triticum aestivum BH1146 young leaves

text: Plasmid

text: pGEM-T


text: University of Victoria

text: live

text: Generic

text: Tissue contributors: Frank Koop, from Charlie Lake BC, Canada

text: kidney, spleen, heart, gill

text: Esox lucius

text: kidney, spleen, heart, gill

text: elucevq mixed_tissue Esox lucius cDNA


text: Korea Research Institute Bioscience & Biotechnology

text: live

text: Generic

text: Macaca mulatta

text: Macaca mulatta library (Kim Y)


text: live

text: Generic

text: Macaca fascicularis

text: Macaca fascicularis library (Kim Y)


text: Smithsonian Institution

text: live

text: Generic

text: cDNA was PCR amplified using a trans-spliced leader primer and a reverse primer from the polyT reverse transcriptase primer. Total RNA was used as a template and sequences from both host and parasite are present in the library. These are putative parasite sequences based on AT >= 60%.

text: Karlodinium veneficum CCMP1975

text: Amoebophrya sp. ex Karlodinium veneficum

text: Amoebophrya ex Karlodinium 0 hr post infection

text: plasmid

text: pGEM-TEZ


text: Smithsonian Institution

text: live

text: Generic

text: Karlodinium veneficum CCMP1975

text: Amoebophrya ex Karlodinium 24 hr post infection

text: plasmid

text: pGEM-TEZ


text: Smithsonian Institution

text: live

text: Generic

text: Karlodinium veneficum CCMP1975

text: Amoebophrya ex Karlodinium 48 hr post infection

text: plasmid

text: pGEM-TEZ


text: Smithsonian Institution

text: live

text: Generic

text: After 60 hours of infection host cells were removed by filtration and parasite spores were incubated for a further 48 hr. cDNA was PCR amplified using a trans-spliced leader primer and a reverse primer from the polyT reverse transcriptase primer. Total RNA was used as a template and sequences from both host and parasite are present in the library. These are putative parasite sequences based on AT >= 60%.

text: Karlodinium veneficum CCMP1975

text: Amoebophrya ex Karlodinium 108 hr post infection

text: plasmid

text: pGEM-TEZ


text: Smithsonian Institution

text: live

text: Generic

text: cDNA was PCR amplified using a trans-spliced leader primer and a reverse primer from the polyT reverse transcriptase primer. Total RNA was used as a template and sequences from both host and parasite are present in the library. These are putative host sequences.

text: Karlodinium veneficum

characteristic: strain

text: Karlodinium veneficum 0 hr post infection

text: plasmid

text: pGEM-TEZ


text: Smithsonian Institution

text: live

text: Generic

characteristic: NCBI submission model

text: Karlodinium veneficum

text: Karlodinium veneficum 24 hr post infection

text: plasmid

text: pGEM-TEZ


text: Smithsonian Institution

text: live

text: Generic

characteristic: NCBI submission package

text: Karlodinium veneficum

text: Karlodinium veneficum 48 hr post infection

text: plasmid

text: pGEM-TEZ


text: Smithsonian Institution

text: live

text: Generic

text: After 60 hours of infection host cells were removed by filtration and parasite spores were incubated for a further 48 hr. cDNA was PCR amplified using a trans-spliced leader primer and a reverse primer from the polyT reverse transcriptase primer. Total RNA was used as a template and sequences from both host and parasite are present in the library. These are putative host sequences.

characteristic: description

text: Karlodinium veneficum

text: Karlodinium veneficum 108 hr post infection

text: plasmid

text: pGEM-TEZ


text: The life science college of Shihezi University

text: live

text: Generic

text: Cucumis sativus

text: EcoRI

text: EcoRI

text: hermaphrodite

characteristic: sex

text: leaf

text: Cucumber leaf SSH library

text: Plasmid

text: pGM-T vector


text: Agricultural Reserch Organization

text: live

text: Generic

text: Colletotrichum gloeosporioides genes regulated by the alkalinization process occur during disease development

text: Colletotrichum gloeosporioides


text: Pochon CHA University

text: live

text: Generic

text: fibroblasts

characteristic: cell type

text: WI-38 VA13

characteristic: cell-line

text: Full-length single stranded cDNA was prepared by SMART synthesis. Suppression subtractive hybridization was used to subtract the SV40-immortalized cells (WI-38 VA13) specific cDNAs. The byproducts of subtraction was amplified by PCR and ligated into the pGEM-T vector. Sequencing was carried out by T7 promoter sequence from cDNA carrying clones selected by blue/white screening.

text: lung

text: Homo sapiens

text: SV40-immortalized lung CHA-V

text: Plasmid

text: pGEM-T Easy vector


text: Max Planck Institute for Chemical Ecology

text: live

text: Generic

text: GeneFishing differential expression analysis

text: Mixed

text: Trichoplusia ni

characteristic: organism

text: Male and Female

text: Mixed

text: Tni GeneFishing

text: Plasmid

text: pCR 2.1 TOPO


text: CRA - Centro di Ricerca per la Frutticoltura di Roma

text: live

text: Generic

text: Naz, Ask, Greco, Red Aleppo, Napoletana, Larnaka, Aegina

characteristic: cultivar

text: E. coli

characteristic: lab-host

text: Flower

text: Pistacia vera

text: Pistacia male and female panicles

characteristic: title

text: Plasmid


characteristic: External Id

text: SINTEF Materials and Chemistry

characteristic: INSDC center name

text: live

text: Generic

text: Copepod subtraction library from whole copepods exposed to diethanolamine (DEA)

text: Adult

characteristic: dev-stage

text: Calanus finmarchicus

text: Whole organism

characteristic: tissue-type

text: Calanomics library 3


text: Universite de Montreal - Faculte de Medecine Veterinaires

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Suppressive subtraction hybridization

text: blastocyst stage

text: uterus

characteristic: organ

text: Neogale vison

text: female

text: American mink differentially expressed cDNAs in the uterus at embryo reactivation after delayed implantation


text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: blastocyst stage

text: embryo

text: Neogale vison

text: American mink differentially expressed cDNAs in the blastocyst at reactivation after delayed implantation


text: Kyushu University

text: live

text: Generic

text: Chlorella hardening-induced ESTs.

characteristic: description

text: L2 stage (ripening phase)

text: Chlorella vulgaris

text: whole cell

text: Chlorella vulgaris whole cell L2 stage (ripening phase)


text: Texas A&M University

text: live

text: Generic

text: Poly(A) mRNA was purified from the Mauriceville-1c mat A strain (FGSC 2225) using the PolyAttract SYSI kit from Promega. Cells were grown in 1X Vogel's medium/0.5% arginine/50(ng/ml) biotin/2% glucose for 4 h at 30 degree C with orbital shaking at 125 rpm. First-strand cDNA containing methyl-C was synthesized using an oligo-dT primer bearing a XhoI site to enable directional cloning. After second-strand synthesis, cDNA was subjected to fractionation using a CL-2B Sepharose size fractionation column. After addition of EcoRI adapters, digestion with XhoI, cDNA with size above 1 kb was ligated to XhoI/EcoRI-digested UNIZAP XR Lambda arms and the ligation products packaged into Lambda particles. The Lambda cDNA library was amplified. Mass-excision of the amplified library was accomplished to produce pBluescript phagemid for cDNA sequencing.

text: Neurospora crassa

text: XhoI

text: EcoRI

text: A

characteristic: sex

text: Mauriceville

characteristic: strain

text: Neurospora crassa cDNA - 4 hours Vegetative Growth in constant light

text: Plasmid

text: pBluescriptSK-

characteristic: vector


text: Queensland University of Technology

text: live

text: Generic

text: Adult

text: Mantle

text: Pinctada maxima

characteristic: organism

text: Pearl oyster mantle library

text: Plasmid

text: pGEM-T Easy