text: live

text: Generic

text: embryo - 9.5 dpc

text: eye

text: Mus musculus

characteristic: organism

text: optic vesicle and lens placode

text: Rashbass mouse MOV 9 5 optic vesicle

text: plasmid (ampicillin resistant)


status: PUBLIC

characteristic: External Id

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: 1st strand cDNA was prepared from medial nasal process tissue, and was then primed with a Not I - oligo(dT) primer. Double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library is normalized. Library was constructed by Bento Soares and M. Fatima Bonaldo.

characteristic: description

text: DH10B (phage resistant)

tag: attribute

characteristic: lab-host

text: Mus musculus

text: medial nasal process

characteristic: tissue-type

characteristic: title

text: phagemid (ampicillin resistant)

characteristic: vector


text: Brigham and Women's Hospital, Harvard Medical School

characteristic: INSDC center name

text: live

text: Generic

text: UB

characteristic: cell-line

text: Mus musculus

text: kidney

text: Mus musculus UB


text: Hainan Medical College

text: live

text: Generic

text: fetal

characteristic: dev-stage

text: Homo sapiens

text: liver

text: Homo sapiens liver fetal


text: Iowa State University

text: live

text: Generic

text: Glycine max

text: Glycine max library (Hermsmeier D)


text: National Institute of Immunology

text: live

text: Generic

text: Labeo catla

text: Catla catla library (Das P)


text: Jawaharlal Nehru University

text: live

text: Generic

text: Labeo rohita

text: spleen

text: Labeo rohita spleen


text: Beijing Medical University

text: live

text: Generic

text: human diploid fibroblasts

characteristic: cell type

text: We screened young cells-related cDNA (yrc) from human diploid fibroblasts (strain 2BS) by differential display at the mRNA level. We did not use any library.

text: young 2BS cells

text: lung

characteristic: organ

text: Homo sapiens

text: female

characteristic: sex

characteristic: strain

text: Human 2BS cDNA


characteristic: External Id

text: Life Science Institute, Sophia University

characteristic: INSDC first public

text: live

text: Generic

text: 6 weeks

text: Rattus norvegicus

characteristic: organism

text: male

text: Wistar

text: brain, hypothalamus

text: Rat Hypothalamus

text: plasmid


text: Harvard Medical School

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: mRNA was purified from embryonic zebrafish hearts (3 day post-fertilization). cDNA was synthesized using a XhoI-Oligo dT adaptor-primer. EcoRI adaptors were ligated, followed by digestion with XhoI, for directional cloning into pre-digested lambda ZAP Express vector.

text: embryonic day 3 post-fertilization

text: E.coli XL1-Blue mrF'

text: heart

text: Danio rerio

text: EcoRI

text: XhoI

text: Zebrafish Embryonic Heart cDNA Library

text: Phage

text: Lambda ZAP Express


text: Institut for Human Genetics

text: live

text: Generic

characteristic: NCBI submission package

text: Human heart cDNA library was constructed in Lambda ZAP II vectors using NotI linkers. Clones from the primary cDNA library were deposited into 96-well trays for storage and retrieval. The 'Isolation of chromosome-specific genes by reciprocal probing of arraed cDNA and cosmid libraries' (Human Molecular Genetics, 1995, Vol. 4, No 8; pp1373-1380) provided cDNA-Clones as plasmids (vector pKSII-, E. coli).

characteristic: description

text: adult

text: E. coli

characteristic: lab-host

text: heart

text: Homo sapiens

text: NotI

text: NotI

text: male

text: heart muscle

text: Human heart cDNA (CCLee)

text: phage-(Lambda ZAP II)-plasmide(pKSII-)-shuttle

text: Lambda ZAP II, pKSII-


text: Purdue University

text: live

text: Generic

text: Glycine max

text: root

characteristic: tissue-type

text: Glycine max root PI89008

characteristic: title


text: Pusan National University

characteristic: INSDC center name

text: live

text: Generic

text: carcinoma

characteristic: cell-line

text: Homo sapiens

text: breast

text: Homo sapiens MCF7


text: CNRS UPR 41, Faculte de Medecine

text: live

text: Generic

text: Homo sapiens

text: Homo sapiens library (Carn G)


text: University of Padova

text: live

text: Generic

text: Homo sapiens

text: placenta

text: Homo sapiens placenta


text: Smith College Department of Biological Sciences

text: live

text: Generic

text: Filarial nematode parasite of cattle. mRNA was prepared from approximately 6,100 L3s isolated from infected mosquitoes from Kumba Cameroon and converted to double-stranded cDNA using reverse transcriptase and oligo(dT) followed by RNase H and DNA pol I. The library has 4.0 x 10E5 independent recombinants and the average insert size is approximately 1kb. The library was constructed by Michelle Lizotte-Waniewski. The library is available from Dr.S.A.Williams, email: genome@smith.edu.

text: infective L3

characteristic: dev-stage

text: XL1-Blue MRF'

text: Onchocerca ochengi

text: Eco RI

text: Xho I

text: Onchocerca ochengi infective larva cDNA (SAW98MLW-OoL3)

text: Phage

text: Lambda Uni-ZAP XR

characteristic: vector


text: live

text: Generic

text: Filarial nematode parasite of humans. mRNA was prepared from approximately 9,000 L2s isolated from infected mosquitoes from Kumba, Cameroon and converted to double-stranded cDNA using reverse transcriptase and oligo(dT) followed by RNase H and DNA pol I. The library has 7.3 x 10E4 independent recombinants and the average insert size is approximately 1kb.The library was constructed by Michelle Lizotte-Waniewski. The library is available from Dr.S.A.Williams, email: genome@smith.edu.

text: XL1-Blue MRF'

text: Onchocerca volvulus

text: Eco RI

text: Xho I

text: Onchocerca volvulus L2 larvae cDNA (SAW98MLW-OvL2)

text: Phage

text: Lambda Uni-ZAP XR


text: live

text: Generic

text: Filarial nematode parasite of humans. mRNA was prepared from approximately 200,000 microfilariae isolated from the skin of infected individuals from Kumba, Cameroon and converted to double-stranded cDNA using reverse transcriptase and oligo(dT) followed by RNase H and DNA pol I. The library has 7.8 x 10E4 independent recombinants and the average insert size is approximately 1kb. The library was constructed by Michelle Lizotte-Waniewski. The library is available from Dr.S.A.Williams, email:genome@smith.edu.

text: microfilaria

text: XL1-Blue MRF'

text: Onchocerca volvulus

text: Eco RI

text: Xho I

text: Onchocerca volvulus microfilaria cDNA (SAW98MLW-OvMf)

text: Phage

text: Lambda Uni-ZAP XR


text: University of Cambridge

text: live

text: Generic

text: Homo sapiens

text: Homo sapiens library (Jene Q)


text: University of Cambridge

text: live

text: Generic

text: fetus

text: Homo sapiens

text: brain

text: Homo sapiens brain fetal (Jene Q)


text: KIST

text: live

text: Generic

text: hepatocyte

characteristic: cell type

text: Mus musculus

text: Mus musculus C57BL hepatocyte


text: The University of Texas MD Anderson Cancer Center

text: live

text: Generic

text: LY-ar

text: Mus musculus

text: Mus musculus LY-ar


text: live

text: Generic

text: LY-as

text: Mus musculus

text: Mus musculus LY-as


text: live

text: Generic

text: Mus musculus

text: Mus musculus library (Kodym R)


text: University of Florida

text: live

text: Generic

text: Bos taurus

text: day 40 pregnant corpus luteum

text: Bos taurus day 40 pregnant corpus luteum


text: University of Florida

text: live

text: Generic

text: Bos taurus

text: 160 day corpus luteum

text: Bos taurus 160 day corpus luteum


text: Oklahoma State University

text: live

text: Generic

text: Essex

characteristic: cultivar

text: PI 437654; mRNA identified in the differential display analysis of soybean-soybean cyst nematode interactions

text: Glycine max

text: roots

text: soybean-soybean cyst nematode differential display


characteristic: External Id

text: University of Michigan

text: live

text: Generic

text: Mus musculus

characteristic: strain

text: Mus musculus C57BL/6J


text: Chemistry Center, Lund University

characteristic: INSDC first public

text: live

text: Generic

text: acute lymphoblastic leukemia pre-B cell

text: Homo sapiens

characteristic: organism

text: Homo sapiens KM3


text: Korea University

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: 18 weeks

text: Homo sapiens

text: fetal brain

text: Homo sapiens fetal brain 18 weeks


text: University of Washington

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Hamburger and Hamilton stage 19-21

text: Gallus gallus

text: White Leghorn

text: whole embryo

text: Gallus gallus whole embryo Hamburger and Hamilton stage 19-21


text: University of Washington

text: live

text: Generic

text: Gallus gallus

text: Gallus gallus library (Peale FV)


text: University of Washington

text: live

text: Generic

text: Hamburger and Hamilton stage 25

text: Gallus gallus

text: White Leghorn

text: cardiac tissue

text: Gallus gallus cardiac tissue Hamburger and Hamilton stage 25


text: LHMC

text: live

text: Generic

text: Homo sapiens

text: Homo sapiens library (Sepe V)


text: Univ. of Chicago

text: live

text: Generic

text: Homo sapiens

text: Homo sapiens library (Yamagata K)


text: Children's Medical Research Institute

text: live

text: Generic

text: Homo sapiens

text: mesothelium

text: Homo sapiens mesothelium


text: Institute of Radiation Medicine, Beijing

text: live

text: Generic

text: SV40-T transfected epithelial cells

text: HBE-M

text: Homo sapiens

text: bronchial

text: Homo sapiens HBE-M


text: INRA

text: live

text: Generic

text: lymphocyte

text: Bos taurus

text: blood

text: Bos taurus blood


text: Cold Spring Harbor Laboratory

text: live

text: Generic

text: This library was supplied by John Doebley (Univ. of Minnesota). cDNAs are directionally cloned into the XhoI and EcoRI sites; XhoI is near the polyA tail. Most reads from this library are made with the reverse universal M13 primer and are 5' in direction. Additional information on this library as well as ftp access to all sequences can be found at http://www.cshl.org/maizegenome

characteristic: description

text: Zea mays

text: XhoI

text: EcoRI

text: glume ear 2 weeks post-pollination

text: maize ear glume library

text: plasmid

text: pBLUESCRIPT SK+ (X52325)


text: Shanghai Institute of Biochemistry

text: live

text: Generic

text: Homo sapiens

text: Homo sapiens (Yang,T.)


text: National Institute of Agrobiological Sciences

text: live

text: Generic

text: Bombyx mori

text: Bombyx mori p50(Daizo)


text: live

text: Generic

text: Bombyx mori

text: Bombyx mori library (Mita K)


text: Institute of Basic Medical Sciences, Peking Union Medical College & Chinese Academy of Medical Sciences

text: live

text: Generic

text: B lymphocytes were isolated from excised human tonsils, activated with PMA(5ng/ml). Total RNA were isolated from resting and activated B lymphocytes reverse transcribed and followed by PCR. The PCR products were displayed on 6% sequencing gel, After autoradiography, differential displayed bands were recovered and confirmed by Northern blot,positive bands were sequenced.

text: Homo sapiens

text: tonsils

characteristic: tissue-type

text: Human tonsils DDRT-PCR


text: Institute of Microbiology

text: live

text: Generic

text: Differentially expressed mRNA from transgenic ripening-altered tomato

text: Solanum lycopersicum

text: Transgenic ripening-altered tomato

characteristic: title


text: Faculte de Medecine Henri Warembourg

characteristic: INSDC center name

text: live

text: Generic

text: epithelial

text: HeLa

text: 2 ug of total RNA was reverse transcribed using the RNA ImageTM mRNA differential display system (GenHunter corporation).

text: Homo sapiens

text: HeLa SRIG (Synthetic retinoids induced genes)

text: plasmid

text: pCR TRAP


text: Institute For Arteriosclerosis Research

text: live

text: Generic

text: monocyte-derived macrophages

text: Homo sapiens

text: Homo sapiens monocyte-derived macrophages


text: MRC

text: live

text: Generic

text: Mus musculus

text: CRH/HA

text: muscle

text: Mus musculus CRH/HA muscle


text: MRC

text: live

text: Generic

characteristic: cell-line

text: Mus musculus

text: Mus musculus CJ7


text: MRC

text: live

text: Generic

text: Mus musculus

text: muscle

text: Mus musculus C3H/HA muscle


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: Plasmid DNA from the normalized library NCI_CGAP_Lu5 was prepared, and ss circles were made in vitro. Following HAP purification, this DNA was used as tracer in a subtractive hybridization reaction. The driver was PCR-amplified cDNAs from a pool of 5,000 clones made from the same library (cloneIDs 1414920-1417991 and 1520904-1522439). Subtraction by Bento Soares and M. Fatima Bonaldo.

characteristic: lab-host

text: lung

characteristic: organ

text: Homo sapiens

text: carcinoid

text: phagemid (ampicillin resistant)


text: College of Natural Sciences, Kyungpook National University

text: live

text: Generic

text: 6 hr to 48 hr

characteristic: dev-stage

text: Danio rerio

text: embryo

text: Danio rerio embryo 6 hr to 48 hr


text: University of Bonn

text: live

text: Generic

text: Sus scrofa

text: skeletal muscle, M. longissimus dorsi

text: Sus scrofa skeletal muscle, M. longissimus dorsi


text: Chonbuk National University

text: live

text: Generic

text: early sexual

text: Aspergillus nidulans

text: Emericella nidulans FGSC4 early sexual


text: Washington University School of Medicine

text: live

text: Generic

text: Re-arrayed Genomic clones. All of the clones are PstI fragments cloned into pBSK+. This library was constructed by Dr. David Grant, USDA-ARS & Iowa State University, G304 Agronomy Hall, Ames, IA 50011-1010, Phone: 515-294-1205, Fax: 515-294-2299, email: dgrant@iastate.edu

text: Glycine max

text: PstI

text: plasmid

text: pBluescript SK+

characteristic: vector


characteristic: External Id

text: live

text: Generic

text: Williams

characteristic: cultivar

text: Immature Cotyledon cDNA. The mRNA was isolated and then Stratagene's cDNA Synthesis Kit (catalog #200401) was used to synthesize the cDNA. First-strand synthesis was performed with 5-methyl dCTP, hence the ligated cDNA is hemimethylated. Stratagene's first-strand synthesis primer was used [GAGAGAGAGAGAGAGAGAGAACTAGTCTCGAG(T)-18]. After second-strand synthesis, the cDNA ends were 'polished' with clone Pfu DNA polymerase, ligated to EcoRI adapters, and phosphorylated. The XhoI site within the first-strand synthesis primer was restricted by digestion with XhoI; all XhoI sites in the cDNA would be protected by their hemimethylated status. The cDNA constructs were size-fractionated with a 500bp cutoff, using GibcoBRL Life Technologie s' cDNA Size Fractionation column. The column eluent was then ligated into Stratagene's pBluescript II XR Predigested vector (pBluescript II SK(+) that had been digested with EcoRI and XhoI, and phosphorylated). Both the white and blue colonies appear to contain recombinant plasmids with cDNA inserts. Blue colonies 9n=15) have been sequenced, and possess putative cDNA inserts.This library was constructed by Dr. Paul Keim & Virginia H. Coryell, Department of Biology, Box5640, Northern Arizona University, Flagstaff, AZ 86011, Phone: 520-523-1078 (Dr. Paul Keim) 520-523-1372 (Virginia H. Coryell) FAX: 520-523-7500 email: paul.keim@nau.edu virginia.coryell@nau.edu and also Dr. Lila Vodkin from the University of Illinois

text: Glycine max

text: EcoRI

text: XhoI

text: immature cotyledon

text: plasmid

text: pBluescript II XR


characteristic: INSDC first public

text: live

text: Generic

text: Williams

text: Root cDNA. The mRNA was isolated from entire roots of 8 day old 'Williams' seedlings which were propagated on paper towels with distilled water. Stratagene's cDNA Synthesis Kit (catalog #200401) was used to synthesize the cDNA. First- strand synthesis was performed with 5-methyl dCTP, hence the ligated cDNA is hemimethylated. Stratagene's first-strand synthesis primer was used [GAGAGAGAGAGAGAGAGAGAACTAGTCTCGAG(T)-18]. After second-strand synthesis, the cDNA ends were 'polished' with clone Pfu DNA polymerase, ligated to EcoRI adapters, and phosphorylated. The XhoI site within the first-strand synthesis primer was restricted by digestion with XhoI; all XhoI sites in the cDNA would be protected by their hemimethylated status. The cDNA constructs were size-fractionated with a 500bp cutoff, using GibcoBRL Life Technologies' cDNA Size Fractionation column. The column eluent was then ligated into Stratagene's pBluescript II XR Predigested vector (pBluescript II SK(+) that had been digested with EcoRI and XhoI, and phosphorylated). Both the white and blue colonies appear to contain recombinant plasmids with cDNA inserts. Blue colonies 9n=15) have been sequenced, and possess putative cDNA inserts. This library was constructed by Dr. Paul Keim & Virginia H. Coryell, Department of Biology, Box5640, Northern Arizona University, Flagstaff, AZ 86011, Phone: 520-523-1078 (Dr. Paul Keim), 520-523-1372 (Virginia H. Coryell), Fax: 520-523-7500, email: paul.keim@nau.edu, virginia.coryell@nau.edu

text: Glycine max

text: EcoRI

text: XhoI

text: root

text: plasmid

text: pBluescript II XR


text: live

text: Generic

text: Williams 82

text: This library was constructed by Dr. Randy C. Shoemaker and Dr. John Erpelding, USDA-ARS Agronomy Department, G401 Agronomy Hall, Iowa State University, Ames, IA 50011-1010 Phone: 515-294-6233 FAX: 515-294-2299 email: rcsshoe@iastate.edu

text: Glycine max

characteristic: organism

text: EcoRI

text: XhoI

text: plasmid

text: pBluescript II XR


text: Agriculture and Agri-Food Canada

characteristic: INSDC last update

text: live

text: Generic

text: Jet Neuf

text: Brassica napus

text: Brassica napus Jet Neuf


text: MAMM, ICRF

text: live

characteristic: INSDC status

text: Generic

text: fibroblast

characteristic: cell type

text: Homo sapiens

text: foreskin

text: Homo sapiens foreskin fibroblast


text: Max-Planck-Institut fuer Molekulare Genetik

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: mixed 5 and 6hrs(early gastrula)

text: Branchiostoma floridae

text: whole embryo

text: pSport1 (Gibco, BRL)


text: Stanford University

text: live

text: Generic

text: Hake lab cDNA library.

characteristic: description

text: P7-P11 leaf

text: E.coli XL1-Blue MFR'

text: shoot

text: Zea mays

text: leaf primordia

characteristic: tissue-type

text: 486 - leaf primordia cDNA library from Hake lab

text: phagemid

text: Lambda zap


text: Shanghai Institute of Physiology, Chinese Academy of Sciences

characteristic: INSDC center name

text: live

text: Generic

text: DDPCR fragment directly cloned in p-GEM T-vector

text: mature

text: Rattus norvegicus

text: female

characteristic: sex

text: Sprague-Dawley

characteristic: strain

text: hippocampus

text: DDPCR Fragment

characteristic: title

text: p-GEM T


text: University of New Mexico

text: live

text: Generic

text: mRNA isolated from germinating conidia, grown in 1x Vogel's, 2% sucrose for 4.5 hours. cDNA directionally cloned into pBluescript SK(-) using the Uni-ZAP XR vector system (Stratagene, La Jolla, CA). Previously identified highly expressed clones were subtracted from this library.

text: Germinating conidia

characteristic: dev-stage

text: E. coli

characteristic: lab-host

text: Neurospora crassa

text: EcoRI

text: XhoI

text: Mating Type A

text: 74-OR23-IV A (FGSC 2489)

text: Conidia

text: Subtracted Conidial

text: Plasmid

text: pBlueScript SK (-)


characteristic: External Id

text: University of New Mexico

text: live

text: Generic

text: mRNA isolated from 5 day old perithecia (fruiting bodies) of the fluffy strain fl a (Mating type a), fertilized with conidia from 74-OR23-IV A (Mating type A). cDNA directionally cloned into pBluescript SK(-) using the Uni-ZAP XR vector system (Stratagene, La Jolla, CA). Previously identified highly expressed clones were subtracted from this library.

text: Fruiting Body

text: E. coli

text: Neurospora crassa

text: EcoRI

text: XhoI

text: Mating type a (fluffy), fertilized

text: fl a (FGSC 4347)

text: Perithecia

text: Subtracted Perithecial

text: Plasmid

text: pBlueScript SK (-)

characteristic: vector


text: University of New Mexico

characteristic: INSDC first public

characteristic: INSDC secondary accession

text: live

text: Generic

characteristic: SRA accession

text: 2% sucrose for 24 hours. cDNA directionally cloned into pBluescript SK(-) using the Uni-ZAP XR vector system (Stratagene, La Jolla, CA). Previously identified highly expressed clones were subtracted from this library.

text: Mycelium

text: E. coli

text: Neurospora crassa

text: EcoRI

text: XhoI

text: Mating type A

text: 74-OR23-IV A (FGSC 2489)

text: Mycelium

text: Subtracted Mycelial

text: Plasmid

text: pBlueScript SK (-)


text: Case Western Reserve University

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: Note: normalization has resulted in an increased frequency of the rare contaminating mouse (host) sequences (~10%). For details of library construction see: Bonaldo MF, Lennon G, Soares MB. Normalization and Subtraction: Two Approaches to Facilitate Gene Discovery. 1996. Genome Research 6:791-806

text: 8 week old adult worms

text: Mus musculus

text: Schistosoma mansoni

characteristic: organism

text: NotI

text: EcoRI

text: mixed

text: Puerto Rican

text: Soares normalized S8W

text: Plasmid


text: Deutsches Krebsforschungszentrum

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Homo sapiens

text: Homo sapiens library (Seranski P)


text: Stanford University

text: live

text: Generic

characteristic: cultivar

text: Hake Lab cDNA library

characteristic: description

text: immature

text: E.coli XL1-Blue

text: shoot

characteristic: organ

text: Zea mays

text: apical meristem

characteristic: tissue-type

text: 487 - apical meristem cDNA library from Hake lab

text: phagemid

text: Lambda zap


text: Smith College Department of Biological Sciences

characteristic: INSDC center name

text: live

text: Generic

text: Lymphatic filarial nematode parasite of humans. mRNA was prepared from approximately 8,000 L3 isolated from mosquitos in Cairo, Egypt and converted to double-stranded cDNA using reverse transcriptase and oligo(dT) followed by RNase H and DNA pol I. The library has 1.0 x 10E6 independent recombinants and the average insert size is ~900 bp. The library was constructed by Michelle. Lizotte-Waniewski. The library is available from Dr.S.A.Williams, email: genome@smith.edu.

text: L3 infective stage larvae

text: XL1-Blue MRF'

text: Wuchereria bancrofti

text: Eco RI

text: Xho I

text: Wuchereria bancrofti L3 cDNA (SAW96MLW-WbL3)

characteristic: title

text: Phage

text: Lambda Uni-ZAP XR


text: University of New Mexico

text: live

text: Generic

text: Westergaard's medium (Nitrogen limiting). Floating mycelial mats grown at 25C for 36 hours. cDNA directionally cloned into pBluescript SK(-) using the Uni-ZAP XR vector system (Stratagene, La Jolla, CA).

text: Unfertilized sexual stage

characteristic: dev-stage

text: E. coli

characteristic: lab-host

text: Neurospora crassa

text: EcoRI

text: XhoI

text: Mating type A

characteristic: sex

text: 74-OR23-IV A (FGSC 2489)

characteristic: strain

text: Unfertilized sexual tissue

text: Westergaards

text: Plasmid

text: pBlueScript SK (-)


characteristic: External Id

text: University of Tokyo

characteristic: INSDC first public

text: live

text: Generic

text: adult

text: Rattus norvegicus

text: Wister

text: gustatory papillae

text: Rattus norvegicus gustatory papillae Wister adult


text: Swedish University of Agricultural Sciences

characteristic: INSDC last update

text: live

text: Generic

text: Arabidopsis thaliana

text: Arabidopsis thaliana library (Josefsson LG)


text: Harvard Medicial School

text: live

characteristic: INSDC status

text: Generic

text: subcloned PCR products

text: Ictalurus punctatus

text: taste tissue

text: Catfish taste tissue subcloned PCR products


text: Massachusetts General Hospital

text: live

text: Generic

text: Suppressive subtractive hybridization was performed using the Clontech PCR Select Kit. Clones generated by this procedure are not full-length nor are they directional. Most clones generated by this procedure contain RsaI sites at both the 5' and 3' ends

text: 2-3 week old

text: Gallus gallus

characteristic: organism

text: eye

text: Chicken eye subtractive hybridization library

text: T/A cloning plasmid

text: pCR2.1 (Invitrogen)

characteristic: vector


text: Massachusetts General Hospital

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: 2-3 week old

text: Gallus gallus

text: eye & pineal

text: Chicken eye & pineal subtractive hybridization library

text: T/A cloning plasmid

text: pCR2.1 (Invitrogen)


text: University Hospital Nijmegen

text: live

text: Generic

text: Subtraction of retina cDNA (tester) was performed with the PCR-Select cDNA Subtraction Kit (Clontech). Driver cDNA consisted of a mixture of cDNAs from brain, kidney, liver, heart, skeletal muscle, placenta, stomach (mucosal layer) and psoas muscle. Tester and driver cDNAs were digested by RsaI; adaptors were ligated to the tester. Two rounds of subtractive hybridization were performed, followed by two suppression PCR reactions. Subtracted PCR products were ligated into a T/A vector (Invitrogen).

characteristic: description

text: Homo sapiens

text: retina

text: subtracted retina cDNA library

text: plasmid

text: pCRII


text: University Hospital Nijmegen

text: live

text: Generic

text: Subtraction of retinal pigment epithelium (RPE) cDNA (tester) was performed with the PCR-Select cDNA Subtraction Kit (Clontech). Driver cDNA consisted of a mixture of cDNAs from brain, kidney, liver, heart, skeletal muscle, placenta, stomach (mucosal layer) and psoas muscle. Tester and driver cDNAs were digested by RsaI; adaptors were ligated to the tester. Two rounds of subtractive hybridization were performed, followed by two suppression PCR reactions. Subtracted PCR products were ligated into a T/A vector (Invitrogen).

text: Homo sapiens

text: retinal pigment epithelium (RPE)

text: subtracted RPE cDNA library

text: plasmid

text: pCRII


text: Biology, University of New Mexico

text: live

text: Generic

text: miracidia and sporocyst

text: Echinostoma paraensei

text: Xho I

text: EcoRI

text: hermaphrodite

text: New Mexico, Loker

text: EpMS Lambda ZAP Express

text: phage

text: Lambda ZAP Express


text: Institute of Animal Breeding and Husbrandry, Christian-Albrechts-University

text: live

text: Generic

text: Mammary gland

characteristic: organ

text: Bos taurus

text: female

text: Mammary gland

text: Bos Taurus Lambda Zap II Library


text: Stanford University

text: live

text: Generic

characteristic: cultivar

text: Wang/Bohnert

text: salt stress

text: E.coli XL Gold

text: shoot

text: Zea mays

text: seedling

characteristic: tissue-type

text: 496 - stressed shoot cDNA library from Wang/Bohnert lab

text: phagemid

text: pBluescriptII SK(+) XR


text: Kwangju Institute of Science & Technology (K-JIST)

characteristic: INSDC center name

text: live

text: Generic

text: The plasmid vector, pSMASH, is a yeast expression vector derived from pGAD424 (Clonetech). It was designed for selective cloning of the genes encoding secreted and membrane proteins. Briefly, it contains EcoRI-NotI subcloning sites followed by a Kex2 protease recognition site and invertase lacking its own methionine and signal sequence.

text: Drosophila melanogaster

text: EcoRI

text: NotI

text: whole embryo

text: Drosophila pSMASH Library

characteristic: title

text: plasmid

text: pSMASH


text: Max-Planck-Institute for Molecular Genetics

text: live

text: Generic

text: oligo(dT) primed, directionally cloned library

text: Strongylocentrotus purpuratus

text: unfertilized egg


text: University of Fukui

text: live

text: Generic

text: Kidney

text: Mus musculus

text: Mouse by PCR analysis

text: plasmid


text: University College London

text: live

text: Generic

text: Cells were grown in TAP (Tris acetate phosphate medium) in continuous weak light and harvested at low density

text: asynchronous vegetative

characteristic: dev-stage

text: Chlamydomonas reinhardtii

text: EcoRI

text: EcoRI

text: mt-

text: Chlamydomonas l gt10

text: Phage

text: l gt10


text: Clemson University

text: live

text: Generic

text: cLED - Tomato Carpel EST Library. OligodT-primed and directionally cloned cDNA in vector Lamda ZAP II with 5' and 3' ends located at the EcoRI and XhoI sites, respectively.

text: 5 days pre-anthesis to 5 days post-anthesis

text: XL1-Blue MRF'

characteristic: lab-host

text: Solanum lycopersicum

text: carpel

text: tomato ovary, TAMU

text: phagemid

text: pBlueScript SK(-)


text: Clemson University

text: live

text: Generic

text: cLEB - Tomato Shoot Meristem EST Library. Oligo-dT primed cDNA library made from tomato vegetative shoots including meristems and small expanding leaves.

text: 8 week old plants

text: XLOLR

text: Solanum lycopersicum

text: shoot meristem

text: tomato shoot, Cornell

text: phagemid


text: Clemson University

text: live

text: Generic

text: R11-13 (Rio Grande x Money Maker)

text: cLES - Tomato Pseudomonas Susceptible EST Library. Directionally cloned cDNAs inserted into pBlueScript SK(-) at 5' end with EcoRI and 3' end with XhoI site

text: 4-week old

text: SOLR

text: Solanum lycopersicum

text: leaf

text: tomato susceptible, Cornell

text: phagemid

text: pBlueScript SK(-)


text: Clemson University

text: live

text: Generic

text: R11-12 (35S::Pto in Rio Grande x Money Maker)

text: cLER - Tomato Pseudomonas Resistant EST Library. Directionally cloned cDNAs inserted into pBlueScript SK(-) at 5' end with EcoRI and 3' end with XhoI site.

text: 4-week old

text: SOLR

text: Solanum lycopersicum

text: leaf

text: tomato resistant, Cornell

text: phagemid

text: pBlueScript SK(-)


text: University of Washington

text: live

text: Generic

text: Directional cDNA library was constructed using Lambda ZP II kit (Stratagene). mRNA was extracted and pooled from normal prostate tissues of 5 individuals (Clontech).

text: Prostate

text: Homo sapiens

text: conorm

text: Plasmid

text: pBluescript


text: Children's Memorial Research Center

text: live

text: Generic

text: The UI-R-C3 library is a subtracted library of a series, ultimately derived from a mixture of individually tagged normalized libraries from rat placenta, adult lung, brain, liver, kidney, heart, spleen, ovary, muscle, and 8, 12 and 18-day embryos, after a series of subtractions to reduce the representation of cDNAs from which ESTs had already been generated. The following serially subtracted libraries were generated in this process: UI-R-C3, UI-R-C2p, UI-R-C1, UI-R-C0, UI-R-A1, UI-R-E1. The tag is a string of 3-5 nucleotides present between the Not I site and the oligo-dT track whichallows identification of the library of origin of a clone within themixture. The subtracted library (UI-R-C3) was constructed as follows: PCRamplified cDNA inserts from UI-R-C2p clones from which 3' ESTs had been derived was used as a driver in a hybridization with the UI-R-C2p library in the form of single-stranded circles. The remaining single-stranded circles (subtracted library) was purified by hydroxyapatite column chromatography, converted to double-stranded circles and electroporated into DH10B bacteria (LifeTechnologies) to generate the UI-R-C3 library. This procedure has been previously described (Bonaldo, Lennon and Soares, Genome Research 6:791-806, 1996)

text: adult

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

characteristic: strain

text: Phagemid


characteristic: External Id

text: Internal Medicine I, University of Ulm

text: live

text: Generic

characteristic: cell-line

text: Homo sapiens

text: pancreatic cancer

text: Human pancreatic cancer cell line PANC1


text: University of Washington

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Directional cDNA library was constructed using Lambda ZP II kit (Stratagene). mRNA was extracted from a fresh prostate tumor tissues (Urology Department, University of Washington).

text: Prostate

text: Homo sapiens

text: bvtumor

text: Plasmid

text: pBluescript


text: University of Washington

text: live

characteristic: INSDC status

text: Generic

text: Directional cDNA library was constructed using Lambda ZP II kit (Stratagene). mRNA was extracted from a frozen prostate tumor tissue (Mayo Clinics).

text: Prostate

text: Homo sapiens

characteristic: organism

text: Plasmid

text: pBluescript


text: University of Washington

text: live

text: Generic

characteristic: NCBI submission model

text: Directional cDNA library was constructed using Lambda ZP II kit (Stratagene). mRNA was extracted from a prostate tumor cell line DU-145.

text: Cell line

text: Homo sapiens

text: Plasmid

text: pBluescript

characteristic: vector


text: University of Washington

text: live

text: Generic

characteristic: NCBI submission package

text: Directional cDNA library was constructed using Lambda ZP II kit (Stratagene). mRNA was extracted from a prostate tumor cell line PC-3.

characteristic: description

text: Cell line

text: Homo sapiens

text: Plasmid

text: pBluescript


text: University of Washington

text: live

text: Generic

text: Directional cDNA library was constructed using Lambda ZP II kit (Stratagene). mRNA was extracted from a prostate tumor cell line LnCap.

text: Cell line

characteristic: organ

text: Homo sapiens

text: LnCap

text: Plasmid

text: pBluescript


text: University of Washington

text: live

text: Generic

text: Directional cDNA library was constructed using Lambda ZP II kit (Stratagene). mRNA was extracted from a primary xenograft prostate tumor in SCIDS mice (UCLA Medical Center).

text: Prostate

text: Mus musculus

text: prsdxg

text: Plasmid

text: pBluescript


text: Lawrence Berkeley National Lab

text: live

text: Generic

text: Sized fractionated cDNAs were directly ligated into pOT2. Plasmid cDNA library.

text: Drosophila melanogaster

text: EcoRI

text: SD Drosophila melanogaster Schneider L2 cell culture pOT2


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: 1st strand cDNA was prepared from pooled lung tumor tissue, and was then primed with a Not I - oligo(dT) primer. Double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library went througb one round of normalization. Library constructed by Bento Soares and M. Fatima Bonaldo.

text: adult

text: DH10B (phage-resistant)

text: lung

text: Homo sapiens

text: squamous cell carcinoma, poorly differentiated (4 pooled tumors, including primary and metastatic)

characteristic: tissue-type

text: phagemid (ampicillin resistant)


characteristic: INSDC center name

text: live

text: Generic

text: mRNA made from lymphoid tissue, cDNA made by oligo-dT priming. Directionally cloned. Size-selected on agarose gel, average insert size 500 bp. Primary library, non-amplified. cDNA Library Preparation: David B. Krizman, Ph.D. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383.

text: bone marrow

text: Homo sapiens

text: CD34+, T negative, patient with chronic myelogenous leukemia

text: plasmid (ampicillin resistant)


text: live

text: Generic

text: bone marrow

text: Homo sapiens

text: CD34+, CD38- from normal bone marrow donor

characteristic: title

text: plasmid (ampicillin resistant)


text: live

text: Generic

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACGAATCTGAAGTGGGAGCGGCCGCCCTTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Eco RI adaptors [5' AATTCACTAGTAAC 3' and 5' GTTACTAGTG 3'], digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library constructed by Bob Barstead.

text: adult, age 27

characteristic: dev-stage

text: DH10B (phage resistant)

text: muscle (psoas)

text: Homo sapiens

text: EcoRI

text: NotI

text: female

characteristic: sex

text: Barstead muscle HPLRB8

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: mRNA made from brain tumor tissue, cDNA made by oligo-dT priming. Directionally cloned. Size-selected on agarose gel, average insert size 500 bp. Primary library, non-amplified. cDNA Library Preparation: David B. Krizman, Ph.D. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383.

characteristic: lab-host

text: brain

text: Homo sapiens

text: oligodendroglioma

text: plasmid (ampicillin resistant)


characteristic: External Id

text: live

text: Generic

text: Total RNA (purified with Trizol and DNAsed before use) was reverse transcribed using a modified oligo-dT primer containing RsaI and HindIII sites. Double-stranded cDNA was digested with RsaI, resulting in blunt ended cDNA of an average 0.1-2 kb in length. Digested cDNA was split into two sets, one used as is as the driver, the other set was split in half again and each half linked to a different adaptor (5'-TCGAGCGGCCGCCCGGGCAGGT-3' or 5'- AGGGCGTGGTGCGGAGGGCGGT-3'), to be used as tester. Subtraction was performed using the Clontech PCR Select cDNA subtraction kit. Pool of three bipolar individuals, male, ages 48 and 50, female, age 37 (S-47, S-83, S-128) subtracted by pool of three schizophrenic males, ages 34, 58 and 60 (S-11, S-64, S-109). Tissues were obtained from the Stanley Neuropathology Consortium (www.stanleylab.org). Library constructed and subtracted by Dr. Nancy Johnston [(410) 614-3918, nlj@welchlink.welch.jhu.edu].

text: DH10B (phage-resistant)

text: brain

text: Homo sapiens

text: EcoRI

text: hippocampus (see description)

text: Stanley Hippocampus BS pool 1

text: plasmid (ampicillin resistant)

text: pCR2.1-TOPO (Invitrogen)


text: live

text: Generic

text: Total RNA (purified with Trizol and DNAsed before use) was reverse transcribed using a modified oligo-dT primer containing RsaI and HindIII sites. Double-stranded cDNA was digested with RsaI, resulting in blunt ended cDNA of an average 0.1-2 kb in length. Digested cDNA was split into two sets, one used as is as the driver, the other set was split in half again and each half linked to a different adaptor (5'-TCGAGCGGCCGCCCGGGCAGGT-3' or 5'- AGGGCGTGGTGCGGAGGGCGGT-3'), to be used as tester. Subtraction was performed using the Clontech PCR Select cDNA subtraction kit. Pool of three mentally normal individuals, male, ages 34 and 48, female, age 50 (S-65, S-67, S-164) subtracted by pool of three schizophrenic males, ages 34, 58 and 60 (S-11, S-64, S-109). Tissues were obtained from the Stanley Neuropathology Consortium (www.stanleylab.org). Library constructed and subtracted by Dr. Nancy Johnston [(410) 614-3918, nlj@welchlink.welch.jhu.edu].

text: DH10B (phage-resistant)

text: brain

text: Homo sapiens

text: EcoRI

text: hippocampus (see description)

text: Stanley Hippocampus NS pool 1

text: plasmid (ampicillin resistant)

text: pCR2.1-TOPO (Invitrogen)


text: University of Washington

text: live

text: Generic

text: Directional cDNA library was constructed using Lambda ZP II kit (Stratagene). mRNA was extracted from a normal prostate base tissue (Urology Department, University of Washington).

text: Prostate

text: Homo sapiens

text: bvnorm

text: Plasmid

text: pBluescript


text: University of Washington

text: live

text: Generic

text: Directional cDNA library was constructed using Lambda ZP II kit (Stratagene). mRNA was extracted from a short-term epithelial culture derived from a normal prostate tissue (Urology Department, University of Washington).

text: Tissue culture

text: Homo sapiens

text: ecnorm

text: Plasmid

text: pBluescript


text: University of Washington

text: live

text: Generic

text: Directional cDNA library was constructed using Lambda ZP II kit (Stratagene). mRNA was extracted from a frozen normal prostate tissue (Mayo Clinic).

text: Prostate

text: Homo sapiens

text: mynorm

text: Plasmid

text: pBluescript


text: University of Washington

text: live

text: Generic

text: Directional cDNA library was constructed using Lambda ZP II kit (Stratagene). mRNA was extracted from a normal prostate tissue provided by a 34-year old organ donor (University of Washington).

text: Prostate

text: Homo sapiens

text: yodnorm

text: Plasmid

text: pBluescript


text: University of Washington

text: live

text: Generic

text: Directional cDNA library was constructed using Lambda ZP II kit (Stratagene). mRNA was extracted from a frozen prostate tumor tissue (Mayo Clinics).

text: Prostate

text: Homo sapiens

text: Plasmid

text: pBluescript


text: University of Washington

text: live

text: Generic

text: Directional cDNA library was constructed using Lambda ZP II kit (Stratagene). mRNA was extracted from a prostate tissue.

text: Prostate

text: Homo sapiens

text: misc

text: Plasmid

text: pBluescript


text: University of California, Riverside

text: live

text: Generic

text: The cDNA was cloned into the SalI/NotI sites of pSPORT1.

text: adult

text: Aedes aegypti

text: SalI

text: NotI

text: female

text: malpighian tubules and gut

text: Aedes aegypti MT pSPORT Library

text: plasmid


text: UMDNJ-NJ Medical School

text: live

text: Generic

text: Identified by differential display.

text: Rattus norvegicus

text: Sprague Dawley

characteristic: strain

text: ocular lens epithelium

text: Rattus norvegicus ocular lens epithelium Sprague Dawley 3-day


text: Catholic University of Leuven

characteristic: INSDC first public

text: live

text: Generic

text: Homo sapiens

text: mediastinal germ cell tumor


text: Coloproctology Center, Takano Hospital

characteristic: INSDC last update

text: live

text: Generic

text: Oncorhynchus mykiss

text: Oncorhynchus mykiss library (Soma G)


text: Kyushu University

text: live

characteristic: INSDC status

text: Generic

text: leukocyte

characteristic: cell type

text: Cyprinus carpio

text: Cyprinus carpio leukocyte library (Fujiki K)


text: Kochi University

text: live

text: Generic

characteristic: NCBI submission model

text: budding stage

text: Polyandrocarpa misakiensis

characteristic: organism

text: white spot

text: Polyandrocarpa misakiensis white spot budding stage


text: Institute for Drug Discovery Research,Yamanouchi Pharmaceutica

text: live

text: Generic

characteristic: NCBI submission package

text: osteoclast

text: primary

characteristic: cell-line

text: 5 day-old

text: Oryctolagus cuniculus

text: long bone

text: osteoclast subtracted library


text: Shizuoka University

text: live

text: Generic

text: maturation stage

text: Citrus unshiu

text: Miyagawa-wase

text: Citrus unshiu Miyagawa-wase maturation stage


text: Kyungpook National University School of Medicine

text: live

text: Generic

text: trophozoite

text: Acanthamoeba healyi

text: OC-3A; ATCC:30866

text: Acanthamoeba healyi cDNA library


text: National Agricultural Science and Technology Institute

text: live

text: Generic

text: Bombyx mori

text: Bombyx mori eggs


text: live

text: Generic

text: Isaria japonica

text: Isaria japonica fruit body


text: STAFF-Institute

text: live

text: Generic

text: adipocyte

text: primary culture

characteristic: description

text: Sus scrofa

text: Sus scrofa adipocyte


text: Children's Memorial Research Center

text: live

text: Generic

text: The UI-R-AA0 library is a non-normalized library constructed from 16.5 dpc rat atrium. The tag is a string of 5 nucleotides present between the Not I site and the oligo-dT track. The library was constructed as described by Bonaldo, Lennon and Soares, Genome Research 6: 791-806, 1996. Tissue provided by Jim Lin, Department of Biology, University of Iowa.

text: adult

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid

characteristic: vector


text: Children's Memorial Research Center

characteristic: INSDC center name

text: live

text: Generic

text: The UI-R-AB0 library is a non-normalized library constructed from 16.5 dpc rat ventricle. The tag is a string of 5 nucleotides present between the Not I site and the oligo-dT track. The library was constructed as described by Bonaldo, Lennon and Soares, Genome Research 6: 791-806, 1996. Tissue provided by Jim Lin, Department of Biology, University of Iowa.

text: adult

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid


text: Children's Memorial Research Center

text: live

text: Generic

text: The UI-R-AC0 library is a non-normalized library constructed from 16.5 dpc rat atrioventricular (AV) canal. The tag is a string of 5 nucleotides present between the Not I site and the oligo-dT track. The library was constructed as described by Bonaldo, Lennon and Soares, Genome Research 6: 791-806, 1996. Tissue provided by Jim Lin, Department of Biology, University of Iowa.

text: adult

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

characteristic: title

text: Phagemid


text: Children's Memorial Research Center

text: live

text: Generic

text: The UI-R-AD0 library is a non-normalized library constructed from 15 dpc rat atrium. The tag is a string of 5 nucleotides present between the Not I site and the oligo-dT track. The library was constructed as described by Bonaldo, Lennon and Soares, Genome Research 6: 791-806, 1996. Tissue provided by Jim Lin, Department of Biology, University of Iowa.

text: adult

characteristic: dev-stage

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid


text: Children's Memorial Research Center

text: live

text: Generic

text: The UI-R-AE0 library is a non-normalized library constructed from 15 dpc rat ventricle. The tag is a string of 5 nucleotides present between the Not I site and the oligo-dT track. The library was constructed as described by Bonaldo, Lennon and Soares, Genome Research 6: 791-806, 1996. Tissue provided by Jim Lin, Department of Biology, University of Iowa.

text: adult

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid


text: Children's Memorial Research Center

text: live

text: Generic

text: The UI-R-AF0 library is a non-normalized library constructed from 15 dpc rat atrioventricular (AV) canal. The tag is a string of 5 nucleotides present between the Not I site and the oligo-dT track. The library was constructed as described by Bonaldo, Lennon and Soares, Genome Research 6: 791-806, 1996. Tissue provided by Jim Lin, Department of Biology, University of Iowa.

text: adult

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid


text: Children's Memorial Research Center

text: live

text: Generic

text: The UI-R-AG0 library is a non-normalized library constructed from 13 dpc rat ventricle. The tag is a string of 6 nucleotides present between the Not I site and the oligo-dT track. The library was constructed as described by Bonaldo, Lennon and Soares, Genome Research 6: 791-806, 1996. Tissue provided by Jim Lin, Department of Biology, University of Iowa.

text: adult

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid


text: Children's Memorial Research Center

text: live

text: Generic

text: The UI-R-G0 library is a normalized library constructed from a mixture of rat tissues (nodose ganglia, dorsal root ganglia, and trigeminal ganglia). The tag is a string of 6 nucleotides present between the Not I site and the oligo-dT track. The library was constructed as described by Bonaldo, Lennon and Soares, Genome Research 6: 791-806, 1996.

text: adult

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid


text: The Johns Hopkins University School of Medicine

text: live

text: Generic

text: c-myc transgenic mouse

text: Mus musculus

text: C57BL/6 x SJL

text: liver

characteristic: tissue-type

text: Mus musculus liver C57BL/6 X SJL


text: Queen's University

text: live

text: Generic

text: 5 day-old

text: Rattus norvegicus

text: Wistar

text: heart

text: Rattus norvegicus heart Wistar 5 day-old


text: Queen's University

text: live

text: Generic

text: 17 day prenatal

text: Rattus norvegicus

text: Wistar

text: heart

text: Rattus norvegicus heart Wistar 17 day prenatal


text: Washington University

text: live

text: Generic

text: Nc-1 strain tachyzoites were propagated in human foreskin fibroblasts in vitro. Directional cDNA was synthesized from poly-A mRNAs by oligo dT priming by Takashi Asai, Washington University. CDNAs were cloned into the EcoRI and XhoI sites of Lambda Uni-Zap using reagents and supplies provided by the supplier (Stratagene). Warning: the library contains a small percentage of cDNAs derived from the human host cells.

text: XL1-Blue MRF'

characteristic: lab-host

text: Neospora caninum

text: EcoRI

text: XhoI

text: Nc-1 Tachyzoite

text: Nc-1Tachyzoite cDNA

text: Lambda Uni-Zap


characteristic: External Id

text: Hamamatsu University School of Medicine

text: live

text: Generic

text: Rattus norvegicus

text: Wistar

text: esophageal mucosa

text: Rattus norvegicus esophageal mucosa


text: LSU Medical Center

text: live

text: Generic

text: fetal

text: Bos taurus

text: pulmonary artery endothelial cell

text: Bos taurus fetal pulmonary artery endothelial cell


text: University of Oulu

text: live

text: Generic

text: Mus musculus

text: EcoRI

text: BALB/c x DBA/2

characteristic: strain

text: Kidney, transgenic mouse overexpressing ODC and AdoMetDC


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

characteristic: INSDC first public

text: live

text: Generic

text: This library represents the normalized version of NCI_CGAP_Brn35. Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.19 kb. Tumor types include: meningioma, oligodendroglioma, astrocytoma (grade II), medulloblastoma, astrocytoma (grade IV). Constructed by Life Technologies.

text: brain

characteristic: organ

text: Homo sapiens

text: SalI

text: NotI

text: tumor, 5 pooled (see description)

text: phagemid (ampicillin resistant)


characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

text: This library represents the normalized version of NCI_CGAP_Ov23. Cloned unidirectionally. Primer: Oligo dT. Average insert size 0.86 kb. Tumor types include: mixed Mullerian tumor, papillary serous, clear cell, spindle cell. All are primary tumors, metastasis positive. Constructed by Life Technologies.

text: ovary

text: Homo sapiens

text: SalI

text: NotI

text: tumor, 5 pooled (see description)

text: phagemid (ampicillin resistant)


text: live

text: Generic

characteristic: NCBI submission model

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.3 kb. 5' adaptor sequence: 5' AATTCGGCACGAG 3' 3' GCCGTGCTC 5' 3' adaptor sequence: 5' (GA)10ACTAGTCTCGAGTTTTTTTTTTTTTTTTTT 3' EcoRI site appears to have been lost in a fraction of the clones. Library constructed by Stratagene; available through Mary May, PhD (Oral and Pharyngeal Cancer Branch, National Institute of Dental and Craniofacial Research, NIH; mmay@yoda.nidr.nih.gov).

text: SOLR (kanamycin resistant)

text: Homo sapiens

text: EcoRI

text: XhoI

text: normal gingiva (cell line from primary keratinocytes)

text: phagemid (ampicillin resistant)

text: Bluescript SK-


text: live

text: Generic

text: SOLR (kanamycin resistant)

text: Homo sapiens

characteristic: organism

text: EcoRI

text: XhoI

text: normal gingiva (cell line from immortalized keratinocytes)

text: phagemid (ampicillin resistant)

text: Bluescript SK-


text: University College London

text: live

text: Generic

characteristic: NCBI submission package

text: The library was grown from cells that were adapting to low CO2 conditions. They were growing auxotrophically on elevated CO2, then dropped to air levels of CO2. The cells were harvested 2 and 2.5 hours later.

text: Chlamydomonas reinhardtii

text: EcoRI

text: XhoI

text: mt+

characteristic: sex

text: wild type 137C

text: Chlamydomonas Uni-Zap XR

text: Phage

text: lambda Uni-Zap XR


text: Cambridge University

text: live

text: Generic

text: Drosophila melanogaster

text: Drosophila melanogaster library (Schwartz YB)


text: University of Texas Southwestern Medical Center at Dallas

text: live

text: Generic

text: syncytiotrophoblast

characteristic: cell type

text: Freshly isolated cytotrophoblast cells from human mid-term placenta were cultured in DMEM containing 2% serum for 4 days and differentiated into syncytiotrophoblast. Cytotrophoblast double-strand cDNAs were used as a driver, and syncytiotrophoblast double-strand cDNA as a tester. The clones from subtracted syncytiotrophoblast cDNA library are expressed at high level in syncytiotrophoblast, and not expressed or expressed at low level in cytotrophoblast cells.

characteristic: description

text: cytotrophoblast cells from mid-term placenta

text: Homo sapiens

text: EcoRI

text: EcoRI

text: placenta

text: Subtracted syncytiotrophoblast cDNA library

text: plasmid

text: pT-Adv


text: University of Minnesota

characteristic: INSDC center name

text: live

text: Generic

text: macrophage

text: Sus scrofa

text: alveoli

text: Sus scrofa alveoli macrophage


text: University of Texas at Austin

text: live

text: Generic

text: seed epidermal cells

text: Texas Marker-1

characteristic: cultivar

text: Gossypium hirsutum

text: Gossypium hirsutum 24 days postanthesis secondary-stage fibers

characteristic: title


text: Lincoln University

text: live

text: Generic

text: neonatal

characteristic: dev-stage

text: Mus musculus

text: skin

characteristic: tissue-type

text: Mus musculus skin C57Bl neonatal


characteristic: External Id

text: Institute of Biomedical Sciences

text: live

text: Generic

text: Homo sapiens

text: lung

text: Homo sapiens lung (Chang L-Y)


text: Institute of Biomedical Sciences

text: live

text: Generic

text: Homo sapiens

text: liver

text: Homo sapiens liver (Chang L-Y)


text: Institute of Biomedical Sciences

text: live

text: Generic

text: Homo sapiens

text: brain

text: Homo sapiens brain (Chang L-Y)


text: Institute of Biomedical Sciences

text: live

text: Generic

text: Homo sapiens

text: kidney

text: Homo sapiens kidney (Chang L-Y)


text: Children's Memorial Research Center

text: live

text: Generic

text: The UI-R-AA1 library is a normalized library constructed from 16.5 dpc rat atrium. The tag is a string of 5 nucleotides present between the Not I site and the oligo-dT track. The library was constructed as described by Bonaldo, Lennon and Soares, Genome Research 6: 791-806, 1996. Tissue provided by Jim Lin, Department of Biology, University of Iowa.

text: adult

text: DH10B (Life Technologies)

characteristic: lab-host

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

characteristic: strain

text: Phagemid

characteristic: vector


text: Children's Memorial Research Center

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: The UI-R-AB1 library is a normalized library constructed from 16.5 dpc rat ventricle. The tag is a string of 5 nucleotides present between the Not I site and the oligo-dT track. The library was constructed as described by Bonaldo, Lennon and Soares, Genome Research 6: 791-806, 1996. Tissue provided by Jim Lin, Department of Biology, University of Iowa.

text: adult

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid


text: Children's Memorial Research Center

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: The UI-R-AC1 library is a normalized library constructed from 16.5 dpc rat atrioventricular (AV) canal. The tag is a string of 5 nucleotides present between the Not I site and the oligo-dT track. The library was constructed as described by Bonaldo, Lennon and Soares, Genome Research 6: 791-806, 1996. Tissue provided by Jim Lin, Department of Biology, University of Iowa.

text: adult

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid


text: Children's Memorial Research Center

text: live

text: Generic

characteristic: NCBI submission package

text: The UI-R-AD1 library is a normalized library constructed from 15 dpc rat atrium. The tag is a string of 5 nucleotides present between the Not I site and the oligo-dT track. The library was constructed as described by Bonaldo, Lennon and Soares, Genome Research 6: 791-806, 1996. Tissue provided by Jim Lin, Department of Biology, University of Iowa.

text: adult

text: DH10B (Life Technologies)

text: Rattus norvegicus

characteristic: organism

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid


text: Children's Memorial Research Center

text: live

text: Generic

text: The UI-R-AE1 library is a normalized library constructed from 15 dpc rat ventricle. The tag is a string of 5 nucleotides present between the Not I site and the oligo-dT track. The library was constructed as described by Bonaldo, Lennon and Soares, Genome Research 6: 791-806, 1996. Tissue provided by Jim Lin, Department of Biology, University of Iowa.

text: adult

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid


text: Children's Memorial Research Center

text: live

text: Generic

text: The UI-R-AF1 library is a normalized library constructed from 15 dpc rat atrioventricular (AV) canal. The tag is a string of 5 nucleotides present between the Not I site and the oligo-dT track. The library was constructed as described by Bonaldo, Lennon and Soares, Genome Research 6: 791-806, 1996. Tissue provided by Jim Lin, Department of Biology, University of Iowa.

text: adult

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid


text: Children's Memorial Research Center

text: live

text: Generic

text: The UI-R-AG1 library is a normalized library constructed from 13 dpc rat ventricle. The tag is a string of 6 nucleotides present between the Not I site and the oligo-dT track. The library was constructed as described by Bonaldo, Lennon and Soares, Genome Research 6: 791-806, 1996. Tissue provided by Jim Lin, Department of Biology, University of Iowa.

text: adult

text: DH10B (Life Technologies)

text: Rattus norvegicus

text: Not I

text: Eco RI

text: Sprague-Dawley

text: Phagemid


text: Instituto de Parasitologia y Biomedicina

text: live

text: Generic

text: epimastigote

characteristic: cell type

text: cDNA library constructed with oligo dt primed epimastigote mRNA and cloned in pt7t318D phagemid with modified polylinker

text: Trypanosoma cruzi

text: EcoRI

text: NotI

text: Cl - Brenner

text: T. Cruzi epimastigote normalised cDNA Library


text: Iowa State University

text: live

text: Generic

text: Templates for the differential-display RT-PCR were reverse-transcribed from total cellular RNA extracted (1) from excised feeding sites of roots with parasitic nematodes attached to it and (2) from adjacent root pieces of the same size supplemented with non-parasitic nematodes. Expression profiles were compared between (1) and (2) and cDNAs displaying differential expression were cloned into the pCR2.1-TOPO TA-vector (Invitrogen).

characteristic: description

text: In-vitro cultures of vegetative plants on media plates

text: Arabidopsis thaliana

text: Ecotype Columbia

text: Excised pieces of roots containing nematode feeding sites

text: Cloned cDNAs of Arabidopsis thaliana generated by mRNA differential-display RT-PCR


text: Iowa State University

characteristic: INSDC center name

text: live

text: Generic

text: pre-parasitic, second-stage, and third-stage parasitic juveniles

text: Heterodera schachtii

text: Sugar-beet cyst nematodes (Heterodera schachtii)

text: Cloned cDNAs of Heterodera schachtii generated by mRNA differential-display RT-PCR


text: Washington University School of Medicine

text: live

text: Generic

text: 1st strand cDNA was primed with an oligo(dT) primer [ATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded cDNA was ligated to a DraIII adaptor [TGTTGGCCTACTGG], digested and cloned into distinct DraIII sites of the pME18S-FL3 vector (5' site CACTGTGTG, 3' site CACCATGTG). XhoI should be used to isolate the cDNA insert. Size selection was performed to exclude fragments <1.5kb. Library constructed by Dr. Sumio Sugano (University of Tokyo Institute of Medical Science) and kindly donated by Dr. Koichi Kawakami. Custom primers for sequencing: 5' end primer CTTCTGCTCTAAAAGCTGCG and 3' end primer CGACCTGCAGCTCGAGCACA.

text: adult

text: DH10B (phage resistant)

text: Danio rerio

text: DraIII (CACTGTGTG)

text: DraIII (CACCATGTG)

text: mixed (one male and one female, including unfertilized eggs)

characteristic: sex

text: AB

text: Sugano Kawakami zebrafish DRA

text: phagemid (ampicillin resistant)


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCCGTCTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library went through one round of normalization. Tissue samples provided by Dr. Brian Dieckgraefe (Washington University, dieck@im.wustl.edu); colonic mucosa represents a range of disease involvement from moderate to severe Crohn's disease; samples include both perforating (fistulas) and non-perforating samples. Library constructed by Bento Soares and M. Fatima Bonaldo.

text: DH10B (phage-resistant)

text: colon

characteristic: organ

text: Homo sapiens

text: Not I

text: Eco RI

text: colonic mucosa from 3 patients with Crohn's disease

characteristic: title

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCTAGCTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library went through one round of normalization. Tissue samples provided by Dr. Brian Dieckgraefe (Washington University, dieck@im.wustl.edu); colonic mucosa represents a range of disease involvement from mild cryptitis to severe ulceration, fibrosis, and degeneration. Library constructed by Bento Soares and M. Fatima Bonaldo.

text: DH10B (phage-resistant)

text: colon

text: Homo sapiens

text: Not I

text: Eco RI

text: colonic mucosa from 5 ulcerative colitis patients

characteristic: tissue-type

text: phagemid (ampicillin resistant)


characteristic: External Id

text: live

text: Generic

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCAACGTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library went through one round of normalization. Library constructed by Bento Soares and M. Fatima Bonaldo.

text: fetal

characteristic: dev-stage

text: DH10B (phage-resistant)

text: thymus, pooled

text: Homo sapiens

text: Not I

text: Eco RI

text: phagemid (ampicillin resistant)


text: National Institute of Diabetes and Digestive and Kidney Diseases

text: live

text: Generic

text: Erythroid Cells

text: Primary Culture of Peripheral Blood Mononuclear Cells

characteristic: cell-line

text: Human peripheral blood mononuclear cells were cultured in the presence or absence of erythropoietin. CD71++++ cells arising only in erythropoietin-supplemented medium were isolated by fluorescence activated cell sorting. Those cells demonstrated an average of six additional doublings in suspension culture and erythroid colony formation in methylcellulose. Suppression subtractive hybridization was used to construct the ad library (tester=sorted CD71++++ cells, driver=unsorted cells cultured without erythropoietin).

text: Progenitor; EPO responsive CD71++++

characteristic: lab-host

text: blood

text: Homo sapiens

text: EcoRI

text: EcoRI

text: blood

text: Hembase; Erythroid Progenitor Cells (LCB:ad library)

text: plasmid

text: pCRII


text: Stanford University

characteristic: INSDC first public

text: live

text: Generic

characteristic: cultivar

text: Seedling stressed root cDNA library from Wang/Bohnert lab

text: salt stress

text: E. coli XL Gold

text: root

text: Zea mays

text: seedling

text: 603 - stressed root cDNA library from Wang/Bohnert lab

text: phagemid

text: pBluescriptII SK(+) XR

characteristic: vector


text: Nankai University

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: fetus

text: Homo sapiens

text: left brain

text: Homo sapiens left brain fetus


text: Stanford University

text: live

text: Generic

characteristic: NCBI submission package

text: Kernel endosperm cDNA library from Schmidt lab

text: 10-14 days post-pollination

text: Kernel

text: Zea mays

characteristic: organism

text: EcoRI

text: XhoI

text: nucellar, embryo, and endosperm

text: 605 - Endosperm cDNA library from Schmidt lab


text: Stanford University

characteristic: INSDC center name

text: live

text: Generic

text: Mixed ear tissue cDNA library from Schmidt lab

characteristic: description

text: ear length from 0.5 cm - 2.0 cm

text: XLOLR (Stratagene)

text: immature ear

text: Zea mays

text: EcoRI

text: XhoI

text: mixed

text: 606 - Ear tissue cDNA library from Schmidt lab

text: phagemid

text: pBK-CMV


text: North Carolina State University

text: live

text: Generic

text: Giant Cells

characteristic: cell type

text: Rutgers Large Red

text: Individual, root-knot induced, tomato giant cells were dissected, and a cDNA bank constructed. This bank was extensively subtracted against healthy root tissue cDNA to generate the current library.

text: E. coli DH12S

text: Root

characteristic: organ

text: Solanum lycopersicum

text: Not I

text: Sfi I

text: Giant cell specific cDNA

text: Phagemid

text: pGem 11Zf+


text: The Institute of Physical and Chemical Research

text: live

text: Generic

text: Arabidopsis thaliana

text: Arabidopsis thaliana library (Shinozaki K)

characteristic: title


characteristic: External Id

text: Metabolex Inc.

text: live

text: Generic

text: This library was created from a mixture of individually prepared, single-tissue libraries. More than 60 libraries were prepared from male and female, fetal and adult, rat tissues.

text: Rattus norvegicus

text: Sprague-Dawley

characteristic: strain

text: Mixed

characteristic: tissue-type

text: Rat mixed-tissue library


text: Kyoto University, Division of Applied Life Sciences

text: live

text: Generic

text: Marchantia polymorpha

text: female

characteristic: sex

text: immature sex organ

text: Marchantia polymorpha immature sex organ


text: Institute of Physical and Chemical Research(RIKEN)

characteristic: INSDC first public

text: live

text: Generic

text: Arabidopsis thaliana

text: Arabidopsis thaliana library (Motohashi R)


text: Tsumura & Co., Kampo Pharmacology Dept.

text: live

text: Generic

text: adult

characteristic: dev-stage

text: Rattus norvegicus

text: female

text: Sprague-Dawley

text: pituitary

text: Rattus norvegicus pituitary Sprague-Dawley adult


text: Academic Medical Centre

text: live

text: Generic

text: 2 years old

text: Homo sapiens

text: female

text: temporal cortex

text: J. Jerspeth, et al., Strategies 5 (1), 1992


text: Institute for Medical Neurobiology, University of Magdeburg 'Otto von Guericke'

text: live

text: Generic

text: neuroblastoma

characteristic: cell-line

text: Mus musculus

text: Mus musculus NEURO-2a


text: MIPS

characteristic: INSDC last update

text: live

text: Generic

text: fetal

characteristic: lab-host

text: Homo sapiens

text: NotI

text: SalI

text: brain

text: 564 (synonym: hfbr2)

characteristic: vector


text: MIPS

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: fetal

text: Homo sapiens

text: NotI

text: SalI

text: brain

text: 547 (synonym: hfbr1)


text: MIPS

characteristic: INSDC center name

text: live

text: Generic

text: fetal

text: Homo sapiens

characteristic: organism

text: NotI

text: SalI

text: kidney

text: 566 (synonym: hfkd2)


text: MIPS

text: live

text: Generic

text: adult

text: Homo sapiens

text: NotI

text: SalI

text: testis

text: 434 (synonym: htes3)


text: MIPS

text: live

text: Generic

text: adult

text: Homo sapiens

text: NotI

text: SalI/MluI

text: uterus

text: 586 (synonym: hute1)


text: Institute of Immunology, National Hospital

text: live

text: Generic

text: activated T cell

characteristic: cell type

text: Homo sapiens

text: Homo sapiens library (Spurkland A)


text: Martin-Luther-University Halle-Wittenberg

text: live

text: Generic

text: Sus scrofa

text: skeletal muscle, M. biceps femoris

text: Sus scrofa skeletal muscle, M. biceps femoris

characteristic: title


characteristic: External Id

text: The Royal Veterinary and Agricultural University

text: live

text: Generic

text: Sus scrofa

text: small intestine

text: directionally cloned cDNA in XL1-blue MRF'


text: European Molecular Biology Laboratory

text: live

text: Generic

text: early neurula

text: Oryzias latipes

text: RZPD 554


text: live

text: Generic

text: early neurula

text: Oryzias latipes

text: Oryzias latipes early neurula


text: LSUMC

text: live

text: Generic

text: Brain

characteristic: organ

text: Mus musculus

text: EcoRI

text: XhoI

text: MATCHMAKER Lex A Mouse Brain Library

text: Plasmid


text: Beijing Institute of Radiation Medicine

text: live

text: Generic

text: Homo sapiens

text: Homo sapiens library (Yu Y)


text: Laboratoire de Chimie Biologique, CNRS UMR 8576

text: live

text: Generic

text: encystation

text: Toxoplasma gondii

text: Toxoplasma gondii encystation, subtracted cDNA

text: T/A cloning vector pCRII(Invitrogen)


text: Beijing Medical University

text: live

text: Generic

text: adult

text: Rattus norvegicus

text: Sprague-Dawley

characteristic: strain

text: brain (nucleus accumbens)

characteristic: tissue-type

text: Rattus norvegicus brain (nucleus accumbens) Sprague-Dawley adult


text: Sun Yat-sen University of Medical Scicences

characteristic: INSDC first public

text: live

text: Generic

text: mRNA was purified from human fetal hearts (8-10 weeks). cDNA was synthesized using a XhoI-Oligo dT adaptor-primer. EcoRI adaptors were ligated, followed by digestion with XhoI, for directional cloning into predigested lambda ZAP Express.

characteristic: description

text: E. coli XL1-Blue

text: Homo sapiens

text: EcoRI

text: XhoI

text: heart

text: Human fetal heart cDNA library

text: Lambda ZAP Express


text: RIKEN

text: live

text: Generic

text: Mus musculus

text: kidney

text: Mus musculus C57BL/6J kidney


text: RIKEN

text: live

text: Generic

text: Mus musculus

text: brain

text: Mus musculus C57BL/6J brain


text: RIKEN

text: live

text: Generic

text: Mus musculus

text: spleen

text: Mus musculus C57BL/6J spleen


text: RIKEN

text: live

text: Generic

text: Mus musculus

text: heart

text: Mus musculus C57BL/6J heart


text: Osaka University

text: live

text: Generic

text: Phaseolus vulgaris

text: Phaseolus vulgaris library (Watanabe T)


text: RIKEN

text: live

text: Generic

text: adult

characteristic: dev-stage

text: Mus musculus

text: male

characteristic: sex

text: brain

text: Mus musculus adult C57BL/6J brain


text: RIKEN

characteristic: INSDC last update

text: live

text: Generic

text: 18-day embryo

text: Mus musculus

text: mixed

text: Mus musculus 18-day embryo C57BL/6J


text: RIKEN

text: live

text: Generic

text: adult

text: Mus musculus

text: lung

text: Mus musculus adult C57BL/6J lung


text: Beijing Medical University

text: live

text: Generic

text: isolated at the time of developed morphine dependence, 10 days and 4 hours after morphine injection

text: adult

text: Rattus norvegicus

text: female

text: Sprague-Dawley

text: nucleus accumbens

text: Rattus norvegicus nucleus accumbens Sprague-Dawley adult


text: Beijing Medical University

text: live

text: Generic

text: adult

text: Rattus norvegicus

text: female

text: Sprague-Dawley

text: periqueductal gray

text: Rattus norvegicus periqueductal gray Sprague-Dawley adult


text: Beijing Medical University

text: live

text: Generic

text: Rattus norvegicus

text: female

text: Sprague-Dawley

text: nucleus accumbens

text: Rattus norvegicus nucleus accumbens Sprague-Dawley


text: The 4th Military Medical University

text: live

text: Generic

characteristic: cell-line

text: sequence isolated by differential analysis in the model of apoptosis, taking apoptotic HL60 treated by nocodazole as tester and untreated HL60 as driver

text: Homo sapiens

text: Homo sapiens HL-60


text: Forestry and Forest Products Research Institute

text: live

characteristic: INSDC status

text: Generic

text: Cryptomeria japonica

text: seedling leaves

text: Cryptomeria japonica seedling leaves


text: Kyushu University

text: live

text: Generic

characteristic: NCBI submission model

text: leukocytes

text: Cyprinus carpio

text: Cyprinus carpio leukocytes


text: RIKEN

text: live

text: Generic

characteristic: NCBI submission package

text: adult

text: Mus musculus

text: liver

text: Mus musculus adult C57BL/6J liver


text: University of Regina

characteristic: INSDC center name

text: live

text: Generic

text: Physcomitrium patens

text: Gametophyte

text: Physcomitrella patens cDNA


text: RIKEN

text: live

text: Generic

text: adult

text: Mus musculus

characteristic: organism

text: male

text: cerebellum

text: Mus musculus adult C57BL/6J cerebellum


text: Royal Institute of Technology

text: live

text: Generic

text: Mus musculus

text: testis

text: Mus musculus testis


text: UCLA

text: live

text: Generic

text: Homo sapiens

text: Homo sapiens library (Nguyen MH)


text: RIKEN

text: live

text: Generic

text: adult

text: Mus musculus

text: female

text: placenta

text: Mus musculus adult C57BL/6J placenta


text: RIKEN

text: live

text: Generic

text: adult

text: Mus musculus

text: male

text: testis

text: Mus musculus adult C57BL/6J testis


text: MRC Human Genetics Unit

text: live

text: Generic

text: Homo sapiens

text: testis

text: Homo sapiens testis (Ladomery MR)


text: Universiti Kebangsaan Malaysia

text: live

text: Generic

text: Second generation merozoites of E. tenella H were purified by column chromatography and mRNA extracted using a FASTTRACK kit (Invitrogen). cDNA was synthesised and a Uni-ZAP XR library was constructed using cDNA synthesis kit, ZAP-cDNA synthesis kit and ZAP-cDNA Gigapack III Gold cloning kit (Stratagene). The library was amplified once through E. coli XL1-Blue MRF'.

text: second generation merozoite

text: XL1-Blue MRF'

characteristic: lab-host

text: Eimeria tenella

text: EcoRI

text: XhoI

text: Houghton

text: Lambda ZAPII

characteristic: vector


characteristic: External Id

text: Nankai University

text: live

text: Generic

text: specific to the development of the left brain

text: fetal

text: Homo sapiens

text: brain

text: Homo sapiens brain fetal (Zhang LD)

characteristic: title


text: Basic Medical Sciences

text: live

text: Generic

text: from drug resistant ovarian cancer cell line

text: Homo sapiens

text: Homo sapiens oc3-VGH


text: CAMS

text: live

text: Generic

text: Rattus norvegicus

text: Wistar

characteristic: strain

text: testes

text: Rattus norvegicus testes Wistar


text: UCLA

text: live

text: Generic

text: endothelial

characteristic: cell type

text: Homo sapiens

text: umbilical vein

characteristic: tissue-type

text: Homo sapiens umbilical vein


text: Emory University

characteristic: INSDC first public

text: live

text: Generic

text: PCR amplified fragment

characteristic: description

text: Mus musculus

text: kidney, brain, and lung

text: Mus musculus kidney, brain, and lung


text: Chinese Academy of Agriculture Science

text: live

text: Generic

text: Oryza sativa

text: male

characteristic: sex

text: Oryza sativa library (Jiang SY)


text: University of Manitoba

characteristic: INSDC last update

text: live

text: Generic

text: Rattus norvegicus

text: SHR

text: kidney

text: Rattus norvegicus kidney SHR


text: University of Manitoba

text: live

text: Generic

text: 12 weeks old

characteristic: dev-stage

text: Rattus norvegicus

text: SHR

text: kidney

text: Rattus norvegicus kidney SHR 12 weeks old


text: European Molecular Biology Laboratory

text: live

characteristic: INSDC status

text: Generic

text: early neurula

text: Oryzias latipes

text: RZPD 555


text: CAMS&PUMC

text: live

text: Generic

characteristic: NCBI submission model

text: cDNA fragment from DDRT-PCR

text: Rattus norvegicus

text: Wistar

text: testis

text: Rattus norvegicus Wistar testis DDRT-PCR


text: live

text: Generic

characteristic: NCBI submission package

text: Anchor enzyme: NlaIII~ This SAGE library is from Colon, colorectal carcinoma cell line (RER-), ATCC: HTB-37. 'Velculescu, V. E., Zhang, L., Vogelstein, B., and Kinzler, K. W. (1995) Serial Analysis of Gene Expression. Science 270, 484-487'

text: Homo sapiens


text: live

text: Generic

text: Anchor enzyme: NlaIII~ This SAGE library is from Normal colonic epithelium. Velculescu, V. E., Zhang, L., Vogelstein, B., and Kinzler, K. W. (1995) Serial Analysis of Gene Expression. Science 270, 484-487

text: Homo sapiens

text: Normal colonic epithelium


text: live

text: Generic

text: Anchor enzyme: NlaIII~ This SAGE library is from Brain, Duke glioblastoma multiforme cell line derived from a 65 yo female. Cells harvested at passage 32. 'Velculescu, V. E., Zhang, L., Vogelstein, B., and Kinzler, K. W. (1995) Serial Analysis of Gene Expression. Science 270, 484-487'

text: Homo sapiens

text: female


text: live

text: Generic

text: Anchor enzyme: NlaIII~ This SAGE library is from an Ovary cell line, Ovarian Clear cell carcinoma cell line ES-2, poorly differentiated. 'Velculescu, V. E., Zhang, L., Vogelstein, B., and Kinzler, K. W. (1995) Serial Analysis of Gene Expression. Science 270, 484-487'

text: Homo sapiens


text: live

text: Generic

text: Anchor enzyme: NlaIII~ This SAGE library is from a Colon, cell line derived from colorectal carcinoma. ATCC: CCL-247. Mutation in the Ras gene, codon 13. Wild type p53. RER+ 'Velculescu, V. E., Zhang, L., Vogelstein, B., and Kinzler, K. W. (1995) Serial Analysis of Gene Expression. Science 270, 484-487'

text: Homo sapiens


text: live

text: Generic

text: Anchor enzyme: NlaIII~ This SAGE library is from Normal colonic epithelium. 'Velculescu, V. E., Zhang, L., Vogelstein, B., and Kinzler, K. W. (1995) Serial Analysis of Gene Expression. Science 270, 484-487'

text: Homo sapiens

text: Normal colonic epithelium


text: live

text: Generic

text: Anchor enzyme: NlaIII~ This SAGE library is from Brain, normal human astrocyte cells harvested at passage 5. 'Velculescu, V. E., Zhang, L., Vogelstein, B., and Kinzler, K. W. (1995) Serial Analysis of Gene Expression. Science 270, 484-487'

text: Homo sapiens


text: live

text: Generic

text: Anchor enzyme: NlaIII~ This SAGE library is from normal, pooled Brain, TB-1017 derived from a 51 yo male and TB-1060 derived from a 17 yo male. 'Velculescu, V. E., Zhang, L., Vogelstein, B., and Kinzler, K. W. (1995) Serial Analysis of Gene Expression. Science 270, 484-487'

text: mixed

text: Homo sapiens

text: both male

text: Brain, normal, pooled.


text: live

text: Generic

text: Anchor enzyme: NlaIII~ This SAGE library is from an Ovary cell line, carcinoma cell line OV1063. 'Velculescu, V. E., Zhang, L., Vogelstein, B., and Kinzler, K. W. (1995) Serial Analysis of Gene Expression. Science 270, 484-487'

text: Homo sapiens

text: Ovary, carcinoma cell line OV1063


text: live

text: Generic

text: Anchor enzyme: NlaIII~ This SAGE library is from an Ovary cell line, carcinoma cell line OVCA432. 'Velculescu, V. E., Zhang, L., Vogelstein, B., and Kinzler, K. W. (1995) Serial Analysis of Gene Expression. Science 270, 484-487'

text: Homo sapiens

text: Ovary, carcinoma cell line OVCA432


text: live

text: Generic

text: Anchor enzyme: NlaIII~ This SAGE library is from Brain, 5 pooled Duke glioblastoma multiforme primary tumors. 'Velculescu, V. E., Zhang, L., Vogelstein, B., and Kinzler, K. W. (1995) Serial Analysis of Gene Expression. Science 270, 484-487'

text: Homo sapiens


text: live

text: Generic

text: Anchor enzyme: NlaIII~ This SAGE library is from Colon, cancer cell line. Wild type p53. RER+ 'Velculescu, V. E., Zhang, L., Vogelstein, B., and Kinzler, K. W. (1995) Serial Analysis of Gene Expression. Science 270, 484-487'

text: Homo sapiens


text: live

text: Generic

text: Anchor enzyme: NlaIII~ This SAGE library is from Colon cancer cell line. Mismatch proficient(RER-) with a mutant p53(248arg -> trp) and a mutant APC. 'Velculescu, V. E., Zhang, L., Vogelstein, B., and Kinzler, K. W. (1995) Serial Analysis of Gene Expression. Science 270, 484-487'

text: Homo sapiens

text: Colon, cancer cell line.


text: live

text: Generic

text: Anchor enzyme: NlaIII~ This SAGE library is from Colon, primary tumor. For more information please see Velculescu, V. E., Zhang, L., Vogelstein, B., and Kinzler, K. W. (1995) Serial Analysis of Gene Expression. Science 270, 484-487 and Zhang L, Zhou W, Velculescu VE, Kern SE, Hruban RH, Hamilton SR, Vogelstein B, Kinzler KW. Gene expression profiles in normal and cancer cells. Science. 1997 May 23;276(5316):1268-72

text: Homo sapiens

text: Colon, primary tumor


text: live

text: Generic

text: Anchor enzyme: NlaIII~ This SAGE library is from Colon, primary tumor. 'Velculescu, V. E., Zhang, L., Vogelstein, B., and Kinzler, K. W. (1995) Serial Analysis of Gene Expression. Science 270, 484-487'

text: Homo sapiens

text: Colon, primary tumor


text: live

text: Generic

text: Anchor enzyme: NlaIII~ This SAGE library is from a Brain cell line, c-myc negative medulloblastoma cell line mhh-1. 'Velculescu, V. E., Zhang, L., Vogelstein, B., and Kinzler, K. W. (1995) Serial Analysis of Gene Expression. Science 270, 484-487'

text: Homo sapiens

text: Brain, c-myc negative medulloblastoma cell line mhh-1


text: live

text: Generic

text: Anchor enzyme: NlaIII~ This SAGE library is from normal Brain, greater than 95% white matter. Post-mortem delay of 3:15. 'Velculescu, V. E., Zhang, L., Vogelstein, B., and Kinzler, K. W. (1995) Serial Analysis of Gene Expression. Science 270, 484-487'

text: Homo sapiens

text: Brain, normal, greater than 95% white matter. Post-mortem delay of 3:15.


text: East Carolina University

characteristic: INSDC center name

text: live

text: Generic

text: Bacteroides fragilis 638R

text: Bacteroides fragilis 638R


text: Monsanto

text: live

text: Generic

text: adult

text: Bos taurus

characteristic: organism

text: breed Holstein

text: mammary gland


text: Monsanto

text: live

text: Generic

text: adult

text: Bos taurus

text: breed Holstein

text: brain hypothalmus


text: University of Hong Kong

text: live

text: Generic

text: Homo sapiens

text: nasopharynx carcinoma

text: Homo sapiens nasopharynx carcinoma


text: University of Hong Kong

text: live

text: Generic

text: epithelial

text: Homo sapiens

text: nasopharynx

text: Homo sapiens nasopharynx


text: Shanghai Institute of Cell Biology

text: live

text: Generic

text: Homo sapiens

text: liver

text: Homo sapiens liver


text: CAMS

text: live

text: Generic

text: Rattus norvegicus

text: Wistar

text: testis

text: Rattus norvegicus testis Wistar


text: Central Research Institute of Roengenology and Radiology

text: live

text: Generic

text: embryonic stem cell

text: CCE

characteristic: cell-line

text: poly(A) RNA was purified from CCE cells treated with retinoic acid for 6 hour. cDNA was constructed using an oligo dT primer and directionally cloned using lambda ZAP II system kit of Stratagene

text: Mus musculus

text: EcoRI

text: XhoI

text: Mus musculus CCE treated with retinoic acid 6 hours

text: pBluescript SK(-)


text: live

text: Generic

text: Anchor enzyme: NlaIII~ This SAGE library is from Brain, Duke glioblastoma multiforme primary tumor derived from a 51 yo male. Velculescu, V. E., Zhang, L., Vogelstein, B., and Kinzler, K. W. (1995) Serial Analysis of Gene Expression. Science 270, 484-487

text: Homo sapiens


text: Hiroshima Univ.

text: live

text: Generic

text: Oncorhynchus mykiss

text: Oncorhynchus mykiss library (Sakamoto T)


text: People's Hospital, Beijing Medical University

text: live

text: Generic

text: Homo sapiens

text: ovary epithelium

text: Homo sapiens ovary epithelium


text: live

text: Generic

text: SKOV3; 3AO

text: Homo sapiens

text: ovary

text: Ovarian cancer cell lines SKOV3; 3AO


text: live

text: Generic

text: embryonic stem cell

text: CCE

text: poly(A) RNA was purified from CCE cells treated with retinoic acid for 23 hour. cDNA was constructed using an oligo dT primer and directionally cloned using lambda ZAP II system kit of Stratagene

text: Mus musculus

text: Eco RI

text: Xho I

text: Mus musculus CCE treated with retinoic acid 23 hours

text: pBluescript SK(-)

characteristic: vector


characteristic: External Id

text: live

text: Generic

text: embryonic stem cell

text: CCE

text: poly(A) RNA was purified from CCE cells that differentiated in suspension for 48 hours (without retinoic acid). cDNA was constructed using an oligo dT primer and directionally cloned using lambda ZAP II system kit of Stratagene

text: Mus musculus

text: Eco RI

text: Xho I

text: Mus musculus CCE without retinoic acid

text: pBluescript SK(-)


text: Internal Medicine I, University of Ulm

text: live

text: Generic

text: down-regulated by TGF-beta1

text: Homo sapiens

text: pancreatic cancer

text: pancreatic cancer cell line PANC1, down-regulated by TGF-beta1

characteristic: title


text: Internal Medicine I, University of Ulm

text: live

text: Generic

text: up-regulated by TGF-beta1

text: Homo sapiens

text: pancreatic cancer

text: pancreatic cancer cell line PANC1, up-regulated by TGF-beta1


text: University of Parma

text: live

text: Generic

text: synonym: Tuber albidum Pico

text: Tuber borchii

characteristic: strain

text: mycelia

text: Tuber borchii mycelia ATCC96540


text: Institute of Radiation Medicine

text: live

text: Generic

text: fetal

text: Homo sapiens

text: kidney

text: Homo sapiens kidney fetal


text: Stanford University

text: live

text: Generic

characteristic: cultivar

text: 3-4 days old root tissue from Walbot Lab (LM)

text: 3-4 days old

text: XLOLR

characteristic: lab-host

text: root

characteristic: organ

text: Zea mays

text: EcoRI

text: XhoI

text: root

text: 614 - root cDNA library from Walbot Lab

text: phagemid

text: pBlueScriptII SK+


text: Cancer Research Institution

characteristic: INSDC first public

text: live

text: Generic

text: Homo sapiens

text: nasopharyngeal carcinoma

characteristic: tissue-type

text: Homo sapiens HNE-1


text: Tokyo University of Marine Science and Technology, Graduate School of Marine Science and Technology

characteristic: INSDC last update

text: live

text: Generic

text: Paralichthys olivaceus

text: Paralichthys olivaceus library (Aoki T)


text: Imperial College School of Medicine

text: live

text: Generic

text: erythroid progenitor

characteristic: cell type

text: Mus musculus

text: Mus musculus erythropoietin regulated EST


text: Fourth Military Medical University

text: live

characteristic: INSDC status

text: Generic

text: IL-2-dependent cytotoxic T lymphocyte

text: CTLL

text: Mus musculus

text: Mus musculus CTLL


text: live

text: Generic

characteristic: NCBI submission model

text: leukocyte infected with Hirame r

text: Paralichthys olivaceus

text: Female

characteristic: sex

text: Leukocyte of Japanese flounder i


text: University of Edinburgh

characteristic: INSDC center name

text: live

text: Generic

characteristic: NCBI submission package

text: Haemonchus contortus

text: intestine

text: Haemonchus contortus Intestinal mRNA


text: The University of Georgia, Department of Plant Biology

text: live

text: Generic

text: The library was made from poly-A RNA in the cloning vector lambda ZAP II. Clones to be sequenced were prepared by mass excision.

characteristic: description

text: rhizome

text: Sorghum halepense

characteristic: organism

text: XhoI

text: EcoRI

text: Plasmid

text: Lambda Zap


characteristic: External Id

text: Sun-Yat-sen University of Medical Sciences

text: live

text: Generic

text: Several hundred adult Schistosoma japonicum(Jiangxi, P.R.China, strain), of mixed sex, were perfused from the mesenteries of experimentally infected rabbits. Double-strain cDNA synthesized with the mRNA isolated from adult worm, was inserted into the bacteriophage lambda gt11 Sfi-Not arms between EcoRI and NotI site of the LacZ gene. The cDNA library was contructed by Chen S.Z. at Nanjing Medical University, Nanjing, Jiangsu, P.R. China. (see: Chen Shuzhen, et al. Chinese Journal of Zoonoses 1997,13(6): 23-25)

text: Schistosoma japonicum

text: EcoRI

text: NotI

text: Mix

text: Chinese

text: Schistosoma japonicum Lambda gt11 Express library

text: Lambda gt11 Sfi-Not

characteristic: vector


text: Brookhaven National Laboratory

text: live

text: Generic

text: Acala Maxxa

text: Six days post anthesis

characteristic: dev-stage

text: Gossypium hirsutum

text: immature fiber

text: Six-day Cotton fiber

characteristic: title

text: Plasmid

text: pBluescript II KS+


text: Faculte de Pharmacie. Universite de Nantes

text: live

text: Generic

text: cloned by differential display method after chemical induction of terminal differentiation of cell line

text: Homo sapiens

text: non-small-cell lung carcinoma

text: Homo sapiens non-small-cell lung carcinoma


text: Gyeongsang National University

text: live

text: Generic

text: average insert size:700 bp; initial pfu:5.400 X 10 9

characteristic: lab-host

text: Brassica rapa subsp. pekinensis

text: EcoRI

text: XhoI

text: Flower bud cDNA

text: pBluescript KS(+)


text: Washington University School of Medicine

text: live

text: Generic

text: Williams

characteristic: cultivar

text: This cDNA library was constructed from mRNA isolated from immature cotyledons of greenhouse grown plants (individual seed fresh weight of 100-300mg). The library was prepared using the Life Technologies pSuperScript cDNA library construction kit. Complementary DNA was synthesized from mRNA using a poly (dT) sequence with a NotI restriction site. SalI linkers adapters were ligated to the blunt-ended cDNA fragments followed by NotI digestion. The cDNA fragments were directionally cloned into the NotI-SalI restriction site of the pSPORT1 vector. The ligated cDNA fragments were transformed into E. coli ElectroMax DH10B host cells. This library was constructed by Dr. Lila Vodkin and Dr. Anu Khanna.

text: Glycine max

text: SalI

text: NotI

text: plasmid


characteristic: INSDC first public

text: live

text: Generic

text: Williams

text: This cDNA library was constructed from mRNA isolated from whole young pods, approximately 2cm long, of 12-week-old greenhouse grown plants. The library was prepared using the Life Technologies pSuperScript cDNA library construction kit. Complementary DNA was synthesized from mRNA using a poly (dT) sequence with a NotI restriction site. SalI linkers adapters were ligated to the blunt-ended cDNA fragments followed by NotI digestion. The cDNA fragments were directionally cloned into the NotI-SalI restriction site of the pSPORT1 vector. The ligated cDNA fragments were transformed into E. coli ElectroMax DH10B host cells. This library was constructed by Dr. Lila Vodkin and Dr. Anu Khanna.

text: Glycine max

text: SalI

text: NotI

text: plasmid


text: live

text: Generic

text: Williams

text: The mRNA was isolated from entire roots of 2-month-old 'Williams' plants that were greenhouse grown in 5-gallon pots. To suppress nodulation, Black Gold All-Purpose potting soil was supplemented with: 0.36g/L available phosphoric acid (P2O5), 20mg/L urea N, 0.16g/L S, 0.49mg/L B, 2.5mg/L Cu, 0.15g/L Fe, 13.53mg/L Mn, 0.26mg/L Mo, 14mg/L Zn, 20mg/L Ca, and the following nutrients in a slow-release form (Osmocote): 0.165g/L ammonia N, 0.185g/L nitrate N, 0.35g/L available phosphoric acid, and 0.35g/L soluble potash. No nodules were visible on the roots at harvest. Stratagene's cDNA Synthesis Kit (catalog #200401) was used to synthesize the cDNA. First-strand synthesis was performed with 5-methyl dCTP, hence the ligated cDNA is hemimethylated. Stratagene's first-strand synthesis primer was used [GAGAGAGAGAGAGAGAGAGAACTAGTCTCGAG(T)-18]. After second-strand synthes is, the cDNA ends were 'polished' with clone Pfu DNA polymerase, ligated to EcoRI adapters, and phosphorylated. The XhoI site within the first-strand synthesis primer was restricted by digestion with XhoI; all XhoI sites in the cDNA would be protected by their hemimethylated status. The cDNA constructs were size-fractionated with a 400bp cutoff, using a SizeSep 400 Spun column from Pharmacia. The column eluent was then ligated into Stratagene's pBluescript II XR Predigested vector (pBluescript II SK(+) that had been digested with EcoRI and XhoI, and phosphorylated). Both the white and blue colonies appear to contain recombinant plasmids with cDNA inserts. This library was constructed by Dr. Paul Keim and Dr. Virginia Coryell.

text: Glycine max

text: EcoRI

text: XhoI

text: plasmid

text: pBluescript II XR


text: live

text: Generic

text: Williams

text: This cDNA library was constructed from mRNA isolated from the apical shoots of 9 to 10 day old etiolated seedlings. The shoot tips including any emerged leaves were harvested for mRNA isolation. The cDNA library was prepared using the Stratagene pBluescript II XR cDNA library construction kit. Complementary DNA was synthesized from mRNA using a primer consisting of a poly (dT) sequence with a XhoI restriction site. EcoRI adapters were ligated to the blunt-ended cDNA fragments followed by XhoI digestion. The cDNA fragments were directionally cloned into the EcoRI-XhoI restriction site of the pBluescript vector. The ligated cDNA fragments were transformed into XL10-Gold host cells. This library was constructed by Dr. Randy Shoemaker and Dr. John Erpelding.

text: Glycine max

text: EcoRI

text: XhoI

text: Apical shoot tips, 9-10 day old etiolated seedlings

characteristic: tissue-type

text: plasmid

text: pBluescript II XR


characteristic: INSDC last update

text: live

text: Generic

text: Williams

text: This cDNA library was constructed from mRNA isolated from whole seedlings of 2-3 week old greenhouse grown plants. The cDNA library was prepared using the Stratagene pBluescript II XR cDNA library construction kit. Complementary DNA was synthesized from mRNA using a primer consisting of a poly (dT) sequence with a XhoI restriction site. EcoRI adapters were ligated to the blunt-ended cDNA fragments followed by XhoI digestion. The cDNA fragments were directionally cloned into the EcoRI-XhoI restriction site of the pBluescript vector. The ligated cDNA fragments were transformed into XL10-Gold host cells. This library was constructed by Dr. Randy Shoemaker and Dr. John Erpelding.

text: Glycine max

text: EcoRI

text: XhoI

text: Whole seedlings, 2-3 week old seedlings, greenhouse grown

text: plasmid

text: pBluescript II XR


text: University of Minnesota

text: live

text: Generic

text: genotype A17

text: cDNA was prepared from polyA+ enriched RNA. The cDNA was directionally ligated into the Unizap XR vector from Stratagene and packaged using Gigapack III Gold packaging extracts. Plasmids containing cDNA inserts were excised from the recombinant lambda-Zap phage using Ex-assist helper phage and propagated in XLOLR cells.

text: 24 hours post-inoculation with Sinorhizobium meliloti

text: E. coli strain XLOLR

text: Medicago truncatula

text: EcoRI

text: XhoI

text: Seedling roots

text: Plasmid

text: pBluescript SK -


text: University of Minnesota

text: live

text: Generic

text: genotype A17

text: Immediately prior to inoculation with Sinorhizobium meliloti (0 hour)

text: E.coli strain XLOLR

text: Medicago truncatula

text: EcoRI

text: XhoI

text: Seedling roots

text: Plasmid

text: pBluescript SK -


text: University of Minnesota

text: live

text: Generic

text: genotype A17

text: cDNA was prepared from polyA+ enriched RNA. The cDNA was directionally ligated into the Unizap XR vector from Stratagene and packaged using Gigapack III Gold packaging extracts. Plasmids containing cDNA inserts were excised from the recombinant lambda-Zap phage using Ex-assist helper phage and propagated in SOLR cells.

text: 2 days post-inoculation with Sinorhizobium meliloti

text: E. coli strain SOLR

text: Medicago truncatula

text: EcoRI

text: XhoI

text: Seedling roots

text: Plasmid

text: pBluescript SK -


text: University of Minnesota

text: live

text: Generic

text: genotype A17

text: 3 days post-inoculation with Sinorhizobium meliloti

text: E. coli strain XLOLR

text: Medicago truncatula

text: EcoRI

text: XhoI

text: Seedling roots

text: Plasmid

text: pBluescript SK -


text: NIOSH

text: live

text: Generic

characteristic: cell-line

text: Homo sapiens

text: bronchial epithelium

text: Homo sapiens BEAS-2B


text: CNR

text: live

characteristic: INSDC status

text: Generic

text: adult

text: Mus musculus

text: hippocampus

text: Mus musculus hippocampus adult