characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: epithelial

text: Anchor enzyme: NlaIII~ Library producer: Aldaz Laboratory~Estrogen receptor positive MCF7 cells were grown in conditions devoid of estrogen for 48h (i.e. IMEM media supplemented with 10% Charcoal Stripped Serum). This SAGE library represents the 0h time point (i.e. baseline, untreated cells) of an estrogen exposure time course experiment.

text: Top10 bacteria

text: Breast

text: Homo sapiens

text: NlaIII

text: BsmF1 as tagging enzyme

text: Female

characteristic: sex

text: Breast Carcinoma - Pleaural effusion

text: plasmid

text: pZero


status: PUBLIC

characteristic: External Id

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: epithelial

tag: attribute

characteristic: cell type

characteristic: cell-line

text: Anchor enzyme: NlaIII~ Library producer: Aldaz Laboratory~MCF7 cells were grown in conditions devoid of estrogen for 48h - (IMEM media supplemented w/ Charcoal Stripped Serum) at time 0h cells were treated with 10-8 M estradiol (E2) - This SAGE library represents the 3h time-point of E2 treatment

characteristic: description

text: Top10 bacteria

characteristic: lab-host

text: Breast

characteristic: organ

text: Homo sapiens

characteristic: organism

text: NlaIII

text: BsmF1 as tagging enzyme

text: Female

text: Breast Carcinoma - Pleaural effusion

characteristic: tissue-type

characteristic: title

text: plasmid

text: pZero

characteristic: vector


text: live

text: Generic

text: epithelial

text: Anchor enzyme: NlaIII~ Library producer: Aldaz Laboratory~MCF7 cells were grown in conditions devoid of estrogen for 48h - (IMEM media supplemented w/ 10% Charcoal Stripped Serum) at time 0h cells were treated with 10-8 M estradiol (E2) - This SAGE library represents the 10h time-point of E2 treatment

text: Top10 bacteria

text: Breast

text: Homo sapiens

text: NlaIII

text: BsmF1 as tagging enzyme

text: Female

text: Breast Carcinoma - Pleaural effusion

text: plasmid

text: pZero


text: The Institute of Physical and Chemical Research (RIKEN)

characteristic: INSDC center name

text: live

text: Generic

text: cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and Genome Science Laboratory in RIKEN. Division of Experimental Animal Research in Riken contributed to prepare mouse tissues. 1st strand cDNA was primed with a primer [5' GAGAGAGAGAAGGATCCAAGAGCTCTTTTTTTTTTTTTTTTVN 3'], cDNA was prepared by using trehalose thermo-activated reverse transcriptase and subsequently enriched for full-length by cap-trapper. cDNA went through one round of normalization to Rot = 20.0 and subtraction to Rot = 370.0. Second strand cDNA was prepared with the primer adapter of sequence [5' GAGAGAGAGATTCTCGAGTTAATTAAATTAATCCCCCCCCCCCCC 3']. cDNA was cleaved with XhoI and BamHI.

text: 12 days embryo

characteristic: dev-stage

text: Mus musculus

text: SalI

text: BamHI

text: RIKEN full-length enriched, 12 days embryo, embryonic body between diaphragm region and neck


text: University of Wolverhampton

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: 48 days post germination

text: Allium sativum

text: shoot

text: Garlic sulphur induced mRNA


characteristic: External Id

text: Medical University of South Carolina

text: live

text: Generic

text: Hemocyte

text: Penaeus vannamei

text: Sfi I

text: Sfi I

text: Hemolymph

text: Plasmid


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

characteristic: INSDC status

text: Generic

text: cDNAs were oligo-dT primed and directionally cloned. Staging according to Nieuwkoop and Faber. Library was constructed by N. Garrett, P. LeMaire, A.M. Zorn, and J.B. Gurdon (Wellcome/CRC Institute). Note: This is a Xenopus Gene Collection (XGC) library.

text: DH10B (phage-resistant)

text: Xenopus laevis

text: NotI

text: EcoRI

text: embryo, animal cap

text: Wellcome CRC pSK animal cap

text: plasmid (ampicillin resistant)

text: pBluescript SK-


text: live

text: Generic

characteristic: NCBI submission model

text: cDNAs were oligo-dT primed and directionally cloned. Library was constructed by N. Garrett, P. LeMaire, A.M. Zorn, and J.B. Gurdon (Wellcome/CRC Institute). Note: This is a Xenopus Gene Collection (XGC) library.

text: DH10B (phage-resistant)

text: Xenopus laevis

text: NotI

text: EcoRI

text: egg

text: Wellcome CRC pSK egg

text: plasmid (ampicillin resistant)

text: pBluescript SK-


text: live

text: Generic

characteristic: NCBI submission package

text: DH10B (phage-resistant)

text: Xenopus laevis

text: NotI

text: EcoRI

text: embryo, stage 10.5

text: Wellcome CRC pSK St 10 5

text: plasmid (ampicillin resistant)

text: pBluescript SK-


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.4 kb. Constructed by Life Technologies. Note: This is a Xenopus Gene Collection (XGC) library.

text: DH10B (phage-resistant)

text: liver

text: Xenopus laevis

text: NotI

text: SalI

text: HICHD XGC Liver

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.55 kb. Constructed by Life Technologies. Note: This is a Xenopus Gene Collection (XGC) library.

text: DH10B (phage-resistant)

text: Xenopus laevis

text: NotI

text: SalI

text: embryo (stage 10)

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.7 kb. Constructed by Life Technologies. Note: This is a Xenopus Gene Collection (XGC) library.

text: DH10B (phage-resistant)

text: Xenopus laevis

text: NotI

text: SalI

text: embryo (stages 24-25)

text: phagemid (ampicillin resistant)


text: live

text: Generic

text: cDNA made by oligo-dT priming. Library constructed in the laboratory of R. Harland, Ph.D. (University of California, Berkeley). Note: This is a Xenopus Gene Collection (XGC) library.

text: DH10B (phage-resistant)

text: ovary

text: Xenopus laevis

text: NotI

text: SalI

text: female

characteristic: sex

text: Harland ovary

text: plasmid (ampicillin resistant, 200 ug/ml)

text: pCS105 (custom)


text: live

text: Generic

text: DH10B (phage-resistant)

text: Xenopus laevis

text: NotI

text: EcoRI

text: embryo, animal cap

text: Wellcome CRC pSK animal

text: plasmid (ampicillin resistant)

text: pBluescript SK-


text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 2.2 kb. Constructed by Life Technologies. Note: This is a Xenopus Gene Collection (XGC) library.

text: DH10B (phage-resistant)

text: Xenopus laevis

text: NotI

text: SalI

text: oocytes

text: phagemid (ampicillin resistant)


text: Washington University School of Medicine

text: live

text: Generic

text: Williams 82

characteristic: cultivar

text: This cDNA library was constructed from mRNA isolated from the senescing leaf tissue of mature greenhouse grown plants of the cultivar Williams 82. Complementary DNA was synthesized from mRNA using a 3' anchored poly(dT) primer. EcoRI adapters were ligated to the blunt ended cDNA fragments followed by digestion with EcoRI and HindIII. The cDNA framents were directionally cloned into the EcoRI-HindIII restriction site of the [T7T3-Pac vector. The ligated cDNA fragments were transformed into DH10B host cells (Gibco BRL). This library was constructed by Dr. Randy Shoemaker and Dr. John Erpelding.

text: mature plants

text: Glycine max

text: EcoRI

text: HindIII

text: Senescing leaves, greenhouse grown

text: plasmid

text: pT7T3Pac (pT7T3, Pharmacia)


text: Morehouse School of Medicine

text: live

text: Generic

text: This is cell-type specific sequence which was elucidated by subtraction assay during mouse spermatogenesis.

characteristic: description

text: Developmental stage

text: Testis

text: Mus musculus

text: EcoRV

text: EcoRV

text: male

text: Swiss Webster

characteristic: strain

text: Mouse testis spermatocyte library

text: pCR3.1 plasmid


text: Wageningen University and Research Center

text: live

text: Generic

text: cDNA was synthesized using SMART III oligo, CDS III oligo d(T)30 (Clontech) and Superscript II reverse transcriptase (Life Technology). cDNA clones were size fractionated and directionally ligated in the Sfi IA restriction site at 5' end and Sfi IB at 3' end of pMAK1. pMAK1 was derived from the plasmid pcDNA II (Invitrogen).

text: second stage preparasitic juveniles (J2)

text: Meloidogyne incognita

text: Sfi IA

text: Sfi IB

text: Meloidogyne incognita J2 (#MD99-1)

text: Plasmid


text: Washington University

text: live

text: Generic

text: two to six-week old

text: Arabidopsis thaliana

text: EcoRI

text: XhoI

text: Columbia

text: aboveground organs

text: Arabidopsis thaliana aboveground organs two to six-week old

text: pBluescriptII SK-


text: Kazusa DNA Research Institute

text: live

text: Generic

text: Arabidopsis thaliana

text: EcoRI

text: XhoI

text: Columbia

text: roots

text: Arabidopsis thaliana roots Columbia

text: pBluescriptII SK-


text: Kazusa DNA Research Institute

text: live

text: Generic

text: Arabidopsis thaliana

text: EcoRI

text: XhoI

text: Columbia

text: flower buds

text: Arabidopsis thaliana flower buds Columbia

text: pBluescriptII SK-


text: Kazusa DNA Research Institute

text: live

text: Generic

text: Arabidopsis thaliana

text: EcoRI

text: XhoI

text: Columbia

text: liquid-cultured seedlings

text: Arabidopsis thaliana liquid-cultured seedlings Columbia

text: pBluescriptII SK-


text: Kazusa DNA Research Institute

text: live

text: Generic

text: Arabidopsis thaliana

text: EcoRI

text: XhoI

text: Columbia

text: green siliques

text: Arabidopsis thaliana green siliques Columbia

text: pBluescriptII SK-


text: The Institute For Genomic Research

text: live

text: Generic

text: Murine adipose, undifferentiated 3T3 cell line

text: undifferentiated 3T3 cell line

text: Mus musculus

text: EcoRI

text: XhoI

text: adipose

text: phagemid

text: pBluescript SK-


text: Clemson University

text: live

text: Generic

text: Morex

text: Plants were grown in the greenhouse at the University of California, Riverside (Fenton, SJ Close, TJ Close). Whole spikes with awns trimmed were collected at 5, 10, 15, 20, 30 and 45 DAP (Fenton). Total RNA was prepared from each pool, equal quantities of all six RNA pools were combined, poly(A) RNA was purified from the mixture, one primary unamplified cDNA library was made, and 1 million pfu were in vivo excised to give pBluescript SK(-) cDNA phagemids (Choi)in the TJ Close lab at the University of California, Riverside. Phagemids were plated and picked at the Clemson University Genomics Institute (CUGI) (Begum, Palmer, Frisch, Atkins and Wing). Plasmid DNA preparations, DNA sequencing and sequence analysis were performed at CUGI (Wing, Yu, Frisch, Henry, Simmons, Oates, Rambo, Main). The sequence has been trimmed to remove vector sequence and contains a minimum of 100 bases of phred value 20 or above. For more details on library preparation and sequence analysis see http://www.genome.clemson.edu/projects/barley. To order this clone see http://www.genome.clemson.edu/orders Also see Close TJ, Wing R, Kleinhofs A, Wise R (2001) Genetically and physically anchored EST resources for barley genomics. Barley Genetics Newsletter 31:29-30. (http://wheat.pw.usda.gov/ggpages/bgn/31/cover.html)

text: SOLR

characteristic: lab-host

text: Hordeum vulgare subsp. vulgare

text: 5-45 DAP Spike

text: Hordeum vulgare 5-45 DAP spike EST library HVcDNA0009 (5 to 45 DAP)

text: Phage

text: lambdaZAP


text: The University of Michigan Medical School

text: live

text: Generic

text: Transcripts identified in a differential display screen.

text: Pituitary gland

characteristic: organ

text: Mus musculus

characteristic: organism

text: Mouse developing pituitary gland

text: Plasmid

text: pGEMT


text: Chongqing University of Medical Sciences

text: live

text: Generic

characteristic: cell-line

text: Homo sapiens

text: Homo sapiens express Library

characteristic: title


text: Changzheng Hospital

characteristic: INSDC center name

text: live

text: Generic

text: Homo sapiens

text: male

text: astrocytoma

characteristic: tissue-type

text: Homo sapiens astrocytoma


text: Korea Univ.

characteristic: INSDC first public

text: live

text: Generic

text: Pyrus pyrifolia

text: Whangkeumbae

text: Pyrus pyrifolia Whangkeumbae


characteristic: INSDC last update

text: live

text: Generic

text: cDNA made by oligo-dT priming. Directionally cloned into EcoRI/XhoI sites using the following 5' adaptor: GGCACGAG(G). Size-selected >500bp for average insert size 1.8kb. Library constructed by Ling Hong in the laboratory of Gerald M. Rubin (University of California, Berkeley) using ZAP-cDNA synthesis kit (Stratagene) and Superscript II RT (Life Technologies).

text: DH10B (phage-resistant)

text: skin

text: Homo sapiens

text: XhoI

text: EcoRI

text: melanotic melanoma

text: plasmid (chloramphenicol resistant, grown at 30C)

characteristic: vector


characteristic: External Id

text: Shanghai Institute of Physiology, Chinese Academy of Sciences

text: live

characteristic: INSDC status

text: Generic

text: It was prepared with suppression subtractive hybridization (SSH).

text: adult

characteristic: dev-stage

text: Columba livia

text: brain

text: Pigeon forebrain cDNA library generated with SSH methodology

text: Plasmid

text: pBluescript(sk-)Vector


text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Anchor enzyme: NlaIII~ This SAGE library is from normal Brain, greater than 95% white matter. Post-mortem delay of 3:15. 'Velculescu, V. E., Zhang, L., Vogelstein, B., and Kinzler, K. W. (1995) Serial Analysis of Gene Expression. Science 270, 484-487'

text: Homo sapiens

text: Brain, normal, greater than 95% white matter. Post-mortem delay of 3:15.


text: live

text: Generic

text: Anchor enzyme: NlaIII~ This SAGE library is from Breast tissue. 'Velculescu, V. E., Zhang, L., Vogelstein, B., and Kinzler, K. W. (1995) Serial Analysis of Gene Expression. Science 270, 484-487'

characteristic: description

text: Homo sapiens

text: Breast


text: live

text: Generic

text: Anchor enzyme: NlaIII~ This SAGE library is from Brain, anaplastic astrocytoma, primary tumor. 'Velculescu, V. E., Zhang, L., Vogelstein, B., and Kinzler, K. W. (1995) Serial Analysis of Gene Expression. Science 270, 484-487'

text: Homo sapiens


text: live

text: Generic

text: cDNA made by oligo-dT priming. Directionally cloned into EcoRI/XhoI sites using the following 5' adaptor: GGCACGAG(G). Size-selected >500bp for average insert size 1.8kb. Library constructed by Ling Hong in the laboratory of Gerald M. Rubin (University of California, Berkeley) using ZAP-cDNA synthesis kit (Stratagene) and Superscript II RT (Life Technologies)

text: DH10B (phage-resistant)

text: colon

text: Homo sapiens

text: XhoI

text: EcoRI

text: adenocarcinoma cell line


text: The Institute of Physical and Chemical Research (RIKEN)

text: live

text: Generic

text: cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and Genome Science Laboratory in RIKEN. Division of Experimental Animal Research in Riken contributed to prepare mouse tissues. 1st strand cDNA was primed with a primer [5' GAGAGAGAGAAGGATCCAAGAGCTCTTTTTTTTTTTTTTTTVN 3'], cDNA was prepared by using trehalose thermo-activated reverse transcriptase and subsequently enriched for full-length by cap-trapper. cDNA went through one round of normalization to Rot = 20.0 and subtraction to Rot = 370.0. Second strand cDNA was prepared with the primer adapter of sequence [5' GAGAGAGAGATTCTCGAGTTAATTAAATTAATCCCCCCCCCCCCC 3']. cDNA was cleaved with XhoI and BamHI. Vector: a modified pBluescript KS(+) after bulk excision from Lambda FLC I.

text: adult

text: Mus musculus

text: SalI

text: BamHI

text: male

characteristic: sex

text: urinary bladder

text: RIKEN full-length enriched, adult male urinary bladder


text: live

text: Generic

text: 16 days neonate

characteristic: lab-host

text: Mus musculus

text: SalI

text: BamHI

text: cerebellum

text: RIKEN full-length enriched, 16 days neonate cerebellum


text: live

text: Generic

text: cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and Genome Science Laboratory in RIKEN. Division of Experimental Animal Research in Riken contributed to prepare mouse tissues. 1st strand cDNA was primed with a primer [5' GAGAGAGAGAAGGATCCAAGAGCTCTTTTTTTTTTTTTTTTVN 3'], cDNA was prepared by using trehalose thermo-activated reverse transcriptase and subsequently enriched for full-length by cap-trapper. cDNA went through one round of normalization to Rot = 10.0 and subtraction to Rot = 185.0. Second strand cDNA was prepared with the primer adapter of sequence [5' GAGAGAGAGATTCTCGAGTTAATTAAATTAATCCCCCCCCCCCCC 3']. cDNA was cleaved with XhoI and BamHI. Vector: a modified pBluescript KS(+) after bulk excision from Lambda FLC I.

text: adult

text: Mus musculus

text: SalI

text: BamHI

text: male

text: bone

text: RIKEN full-length enriched, adult male bone


text: Victorian Institute of Animals Science

text: live

text: Generic

text: Adult

text: Oesophagostomum dentatum

text: Differentially expressed Oesophagostomum dentatum mRNAs


text: BioPia

text: live

text: Generic

text: Panax ginseng

text: hairy root

text: Panax ginseng hairy root


text: live

text: Generic

text: adult

text: Mus musculus

text: SalI

text: BamHI

text: female

text: vagina

text: RIKEN full-length enriched, adult female vagina


text: live

text: Generic

text: 6 days neonate

text: Mus musculus

text: SalI

text: BamHI

text: skin

text: RIKEN full-length enriched, 6 days neonate skin


text: live

text: Generic

text: 16 days neonate

text: Mus musculus

text: SalI

text: BamHI

text: thymus

text: RIKEN full-length enriched, 16 days neonate thymus


text: live

text: Generic

text: cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and Genome Science Laboratory in RIKEN. Division of Experimental Animal Research in Riken contributed to prepare mouse tissues. 1st strand cDNA was primed with a primer [5' GAGAGAGAGAAGGATCCAAGAGCTCTTTTTTTTTTTTTTTTVN 3'], cDNA was prepared by using trehalose thermo-activated reverse transcriptase and subsequently enriched for full-length by cap-trapper. cDNA went through one round of normalization to Rot = 20.0 and subtraction to Rot = 459.0. Second strand cDNA was prepared with the primer adapter of sequence [5' GAGAGAGAGATTCTCGAGTTAATTAAATTAATCCCCCCCCCCCCC 3']. cDNA was cleaved with XhoI and BamHI. Vector: a modified pBluescript KS(+) after bulk excision from Lambda FLC I.

text: adult

text: Mus musculus

text: SalI

text: BamHI

text: male

text: hypothalamus

text: RIKEN full-length enriched, adult male hypothalamus


text: live

text: Generic

text: cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and Genome Science Laboratory in RIKEN. Division of Experimental Animal Research in Riken contributed to prepare mouse tissues. 1st strand cDNA was primed with a primer [5' GAGAGAGAGAAGGATCCAAGAGCTCTTTTTTTTTTTTTTTTVN 3'], cDNA was prepared by using trehalose thermo-activated reverse transcriptase and subsequently enriched for full-length by cap-trapper. cDNA went through one round of normalization to Rot = 10.0 and subtraction to Rot = 459.0. Second strand cDNA was prepared with the primer adapter of sequence [5' GAGAGAGAGATTCTCGAGTTAATTAAATTAATCCCCCCCCCCCCC 3']. cDNA was cleaved with XhoI and BamHI. Vector: a modified pBluescript KS(+) after bulk excision from Lambda FLC I.

text: adult

text: Mus musculus

text: SalI

text: BamHI

text: male

text: spinal cord

text: RIKEN full-length enriched, adult male spinal cord


text: University of Virginia

text: live

text: Generic

text: newborn

text: Mus musculus

text: ICR

characteristic: strain

text: brain

text: Stratagene lambda ZAP II libraries, Cat number 936309


text: Washington University School of Medicine

text: live

text: Generic

text: Ogden

characteristic: cultivar

text: This cDNA library was constructed from mRNA isolated from 2 week old seedlings with the cotyledons removed at the time of harvest. The seedlings for the cultivar Ogden were grown in a growth chamber using germination paper. Complementary DNA was synthesized from mRNA using a primer consisting of a poly(dT) sequence with a XhoI restriction site. EcoRI adapters were ligated to the blunt-ended cDNA fragments followed by XhoI digestion. The cDNA fragments were directionally cloned into the EcoRI-XhoI restriction site of the pBluescript vector. The ligated cDNA fragments were transformed into DH10B host cells (Gibco BRL). This library was constructed by Dr. Randy Shoemaker.

text: Glycine max

characteristic: organism

text: EcoRI

text: XhoI

text: whole seedling without cotyledons

text: plasmid

text: pBluescriptII SK+


text: live

text: Generic

text: Williams 82

text: This cDNA library was constructed from mRNA isolated from hypocotyl and plumule tissues of seeds germinated for three days of the cultivar Williams 82. Complementary DNA was synthesized from mRNA using a primer consisting of a poly(dT) sequence with a NotI restriction site. EcoRI adapters were ligated to the blunt-ended cDNA fragments followed by digestions with EcoRI and NotI. The cDNA fragments were directionally cloned into the EcoRI-NotI restriction site of the pT7T3-Pac vector. The ligated cDNA fragments were transformed into DH10B host cells (Gibco BRL). This library was constructed by Dr. Randy Shoemaker.

text: Glycine max

text: EcoRI

text: NotI

text: Hypocotyl and Plumule, germinating seeds

characteristic: title

text: plasmid

text: pT7T3Pac (Pharmacia)


text: Auburn University

characteristic: INSDC center name

text: live

text: Generic

text: Brain

characteristic: organ

text: Ictalurus punctatus

text: NotI

text: SalI

text: Brain cDNA library

text: Phagemid


text: USDA-ARS

characteristic: INSDC first public

text: live

text: Generic

text: 9 day old seedlings

text: Triticum aestivum

text: leaf

characteristic: tissue-type

text: Triticum aestivum Lambda Zap


text: Friedrich-Alexander-Universitaet Erlangen-Nuernberg

characteristic: INSDC last update

text: live

text: Generic

text: CD4 positive T-lymphocytes

characteristic: cell type

text: adult T-cell leukemia cell line JuanaW

characteristic: cell-line

text: Homo sapiens

text: Homo sapiens adult T-cell leukemia cell line JuanaW


characteristic: External Id

text: The Institute of Physical and Chemical Research (RIKEN)

text: live

characteristic: INSDC status

text: Generic

text: 0 day neonate

characteristic: dev-stage

text: Mus musculus

text: SalI

text: BamHI

text: thymus

text: RIKEN full-length enriched, 0 day neonate thymus


text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

characteristic: description

text: adult

text: Mus musculus

text: SalI

text: BamHI

text: male

text: aorta and vein

text: RIKEN full-length enriched, adult male aorta and vein


text: Universite du Quebec a Montreal

text: live

text: Generic

text: Musketeer

text: Secale cereale

text: Leaf

text: Rye cDNA library

text: UniZap

characteristic: vector


text: Kansas State University

text: live

text: Generic

text: Chinese spring

characteristic: cultivar

text: Total RNA was isolated from wheat tissues (leaves, anthers, ovaries) before anathesis and used for synthesis of differential expressed fragments among these tissues. Fragments that showed differential expression were recovered from gells, amplified, and cloned in pGM-T vector. Cloned cDNAs were sequenced with T7 or SP6 standard primers

text: preanathesis

text: Triticum aestivum

text: Leaves

text: Differential display fragments L

text: pGM-T


text: live

text: Generic

text: 3 days neonate

characteristic: lab-host

text: Mus musculus

characteristic: organism

text: SalI

text: BamHI

text: thymus

text: RIKEN full-length enriched, 3 days neonate thymus


characteristic: INSDC center name

text: live

text: Generic

text: 7 days neonate

text: Mus musculus

text: SalI

text: BamHI

text: cerebellum

text: RIKEN full-length enriched, 7 days neonate cerebellum

characteristic: title


characteristic: INSDC first public

text: live

text: Generic

text: cDNA made by oligo-dT priming. Directionally cloned into EcoRI/XhoI sites using the following 5' adaptor: GGCACGAG(G). Library constructed by Ling Hong in the laboratory of Gerald M. Rubin (University of California, Berkeley) using ZAP-cDNA synthesis kit (Stratagene) and Superscript II RT (Life Technologies). Note: this is a NIH_MGC Library.

text: DH10B (phage-resistant)

text: eye

characteristic: organ

text: Homo sapiens

text: XhoI

text: EcoRI

text: retinoblastoma

text: plasmid (chloramphenicol resistant, grown at 30C)


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

characteristic: INSDC last update

text: live

text: Generic

text: DH10B (phage-resistant)

text: brain

text: Homo sapiens

text: XhoI

text: EcoRI

text: neuroblastoma

characteristic: tissue-type


text: Louisiana State University, Health Sciences Center

text: live

text: Generic

text: Epileptic model

text: Rattus norvegicus

text: Sprague-Dawley

characteristic: strain

text: Hippocampus

text: Rat hippocampus cDNA


characteristic: External Id

text: Clemson University

text: live

text: Generic

text: CI16151 (Mla6)

text: C.I. 16151 (Mla6) plants were greenhouse grown in the R Wise lab at Iowa State University, Ames, IA; 7 day old green seedlings were challenged with isolate 5874 (AvrMla6) of Blumeria graminis f. sp. hordei, and leaves were harvested 20 and 24 hr post-inoculation and snap frozen; uninoculated leaves were harvested 20 hr post-inoculation (Wei, Wise). In the TJ Close lab at the University of California, Riverside, total RNA was prepared from each sample pool, equal quantities of all three RNA pools were combined, poly(A) RNA was purified from the mixture, one primary unamplified cDNA library was made, and 1 million pfu were in vivo excised to give pBluescript SK(-) cDNA phagemids (Choi, Close). Phagemids were plated and picked at the Clemson University Genomics Institute (CUGI) (Begum, Palmer, Frisch, Atkins and Wing). Plasmid DNA preparations, DNA sequencing and sequence analysis were performed at CUGI (Wing, Yu, Frisch, Henry, Simmons, Oates, Rambo, Main). The sequence has been trimmed to remove vector sequence and contains a minimum of 100 bases of phred value 20 or above. For more details on library preparation and sequence analysis see http://www.genome.clemson.edu/projects/barley. To order this clone see http://www.genome.clemson.edu/orders Also see Close TJ, Wing R, Kleinhofs A, Wise R (2001) Genetically and physically anchored EST resources for barley genomics. Barley Genetics Newsletter 31:29-30. (http://wheat.pw.usda.gov/ggpages/bgn/31/cover.html)

text: SOLR

text: Hordeum vulgare subsp. vulgare

text: seedling green leaf

text: Hordeum vulgare seedling green leaf EST library HVcDNA0005 (Blumeria challenged)

text: Phage

text: lambdaZAP


text: University of Notre Dame

text: live

characteristic: INSDC status

text: Generic

text: Adult

text: Rhagoletis pomonella

text: R. pomonella cDNA Lambda-Zap Library (PL1)

text: Phage


text: FBN Dummerstorf

text: live

text: Generic

text: lactation

characteristic: dev-stage

text: Bos taurus

text: female

characteristic: sex

text: Intestine

text: Cattle pUC18 Library

text: Plasmid

text: pUC18 Sma I/BAP


text: Chinese PLA General Hospital

text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: Homo sapiens

text: stomach cancer

text: Homo sapiens stomach cancer


text: live

text: Generic

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCATCACTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library constructed and normalized by Bento Soares and M.Fatima Bonaldo.

characteristic: description

text: DH10B (phage-resistant)

text: brain

text: Homo sapiens

text: NotI

text: EcoRI

text: oligodendroglioma

text: phagemid (ampicillin resistant)

characteristic: vector


text: live

text: Generic

text: Double-stranded cDNA was prepared from cell line RNA. 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.65 kb (range 0.9-3.0 kb). This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA).

text: DH10B (T1 phage-resistant)

characteristic: lab-host

text: placenta

text: Homo sapiens

text: SfiI (ggccgcctcggcc)

text: SfiI (ggccattatggcc)

text: choriocarcinoma, cell line

text: pDNR-NCI (Clontech)


text: live

text: Generic

text: Double-stranded cDNA was prepared from cell line RNA. 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.55 kb (range 0.9-4.0 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA).

text: DH10B (T1 phage-resistant)

text: bladder

text: Homo sapiens

characteristic: organism

text: SfiI (ggccgcctcggcc)

text: SfiI (ggccattatggcc)

text: carcinoma, cell line

text: pDNR-LIB (Clontech)


text: Harvard University, Howard Hughes Medical Institute

characteristic: INSDC center name

text: live

text: Generic

text: Library constructed using SuperScript Plasmid Library kit (Life Technologies). cDNA made by oligo-dT priming. Size-selected by column fractionation; average insert size 1.47 kb. Primary library, unamplified. cDNA Library Preparation: Guolin Chen.

text: Embryonic day 10.5 and 12.5, mixed

text: Pancreas

text: Mus musculus

text: Not 1

text: Sal 1

text: Both

text: ICR

text: Pancreatic Bud

text: Mouse E10 5 12 5 Pancreas cDNA Library

text: plasmid (ampicillin resistant)


text: Hainan Medical College

text: live

text: Generic

text: Cocos nucifera

text: embryo

text: Cocos nucifera embryo

characteristic: title


text: Institute of Genetic Diagnosis, the Fourth Military Medical University

characteristic: INSDC first public

text: live

text: Generic

text: Homo sapiens

text: Homo sapiens library (Zhao JR)


text: Cornell University

text: live

text: Generic

text: Approximately 1,000 ovaries were hand dissected from adult C. elegans. RNA was extracted and the cDNA was amplified using the SMART PCR cDNA Library Construction Kit (Clontech). This was ligated into Lambda Zap.

text: ovary

characteristic: organ

text: Caenorhabditis elegans

text: hermaphrodite

text: C. elegans ovary cDNA library in Lambda Zap

text: Phage

text: Lambd Zap II


text: The Institute of Physical and Chemical Research (RIKEN)

characteristic: INSDC last update

text: live

text: Generic

text: cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and Genome Science Laboratory in RIKEN. Division of Experimental Animal Research in Riken contributed to prepare mouse tissues. 1st strand cDNA was primed with a primer [5' GAGAGAGAGAAGGATCCAAGAGCTCTTTTTTTTTTTTTTTTVN 3'], cDNA was prepared by using trehalose thermo-activated reverse transcriptase and subsequently enriched for full-length by cap-trapper. cDNA went through one round of normalization to Rot = 20.0 and subtraction to Rot = 459.0. Second strand cDNA was prepared with the primer adapter of sequence [5' GAGAGAGAGATTCTCGAGTTAATTAAATTAATCCCCCCCCCCCCC 3']. cDNA was cleaved with XhoI and BamHI. Vector: a modified pBluescript KS(+) after bulk excision from Lambda FLC I.

text: 10 days neonate

text: Mus musculus

text: SalI

text: BamHI

text: cortex

text: RIKEN full-length enriched, 10 days neonate cortex


text: live

text: Generic

text: cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and Genome Science Laboratory in RIKEN. Division of Experimental Animal Research in Riken contributed to prepare mouse tissues. 1st strand cDNA was primed with a primer [5' GAGAGAGAGAAGGATCCAAGAGCTCTTTTTTTTTTTTTTTTVN 3'], cDNA was prepared by using trehalose thermo-activated reverse transcriptase and subsequently enriched for full-length by cap-trapper. cDNA went through one round of normalization to Rot = 20.0 and subtraction to Rot = 459.0. Second strand cDNA was prepared with the primer adapter of sequence [5'GAGAGAGAGATTCTCGAGTTAATTAAATTAATCCCCCCCCCCCCC 3']. cDNA was cleaved with XhoI and BamHI. Vector: a modified pBluescript KS(+) after bulk excision from Lambda FLC I. ~Retina RNA was provided by Stefano Gustincich, Department of Neurobiology, Harvard Medical School, 220 Longwood Ave., Boston, MA02115, USA, whose assistance we gratefully acknowledge.

text: adult

text: Mus musculus

text: SalI

text: BamHI

text: retina

characteristic: tissue-type

text: RIKEN full-length enriched, adult retina


text: live

text: Generic

text: cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and Genome Science Laboratory in RIKEN. Division of Experimental Animal Research in Riken contributed to prepare mouse tissues. 1st strand cDNA was primed with a primer [5' GAGAGAGAGAAGGATCCAAGAGCTCTTTTTTTTTTTTTTTTVN 3'], cDNA was prepared by using trehalose thermo-activated reverse transcriptase and subsequently enriched for full-length by cap-trapper. Second strand cDNA was prepared with the primer adapter of sequence [5' GAGAGAGAGATTCTCGAGTTAATTAAATTAATCCCCCCCCCCCCC 3']. cDNA was cleaved with XhoI and BamHI. Vector: a modified pBluescript KS(+) after bulk excision from Lambda FLC I.

text: 2 cells

text: Mus musculus

text: SalI

text: BamHI

text: egg

text: RIKEN full-length enriched, 2 cells egg


text: live

text: Generic

text: cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and Genome Science Laboratory in RIKEN. Division of Experimental Animal Research in Riken contributed to prepare mouse tissues. 1st strand cDNA was primed with a primer [5' GAGAGAGAGAAGGATCCAAGAGCTCTTTTTTTTTTTTTTTTVN 3'], cDNA was prepared by using trehalose thermo-activated reverse transcriptase and subsequently enriched for full-length by cap-trapper. cDNA went through one round of subtraction to Rot = 229.0 Second strand cDNA was prepared with the primer adapter of sequence [5' GAGAGAGAGATTCTCGAGTTAATTAAATTAATCCCCCCCCCCCCC 3']. cDNA was cleaved with XhoI and BamHI. Vector: a modified pBluescript KS(+) after bulk excision from Lambda FLC I.

text: 9.5 days embryo

text: Mus musculus

text: SalI

text: BamHI

text: parthenogenote

text: RIKEN full-length enriched, 9.5 days embryo parthenogenote


text: live

text: Generic

text: adult

text: Mus musculus

text: SalI

text: BamHI

text: male

text: corpora quadrigemina

text: RIKEN full-length enriched, adult male corpora quadrigemina


text: School of Medicine, University of Southern California

text: live

text: Generic

text: Macaca mulatta

text: balloon-injured carotid artery

text: Macaca mulatta balloon-injured carotid artery


text: live

text: Generic

text: Macaca mulatta

text: normal carotid artery

text: Macaca mulatta normal carotid artery


text: University of Edinburgh

text: live

text: Generic

text: Constructed by David Knox, Moredun Institute, Edinburgh. Primary library titre was 4x10^6 pfu.

text: Adult, day 11

text: Haemonchus contortus

text: EcoRI

text: XhoI

text: Mixed

text: Moredun

characteristic: strain

text: Haemonchus contortus d11 mixed adult from David Knox

text: Phage

text: Lambda Zap II


characteristic: External Id

text: University of Nebraska

text: live

text: Generic

text: EST's isolated from differential display experiment

text: follicular phase

text: Sus scrofa

text: female

text: Anterior pituitary

text: Pig anterior pituitary cDNA


text: Universite du Quebec a Montreal

text: live

characteristic: INSDC status

text: Generic

text: Norstar

characteristic: cultivar

text: Triticum aestivum

text: Leaf

text: Wheat cDNA library

text: Lambda Zap II


text: live

text: Generic

text: cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and Genome Science Laboratory in RIKEN. Division of Experimental Animal Research in Riken contributed to prepare mouse tissues. 1st strand cDNA was primed with a primer [5' GAGAGAGAGAAGGATCCAAGAGCTCTTTTTTTTTTTTTTTTVN 3'], cDNA was prepared by using trehalose thermo-activated reverse transcriptase and subsequently enriched for full-length by cap-trapper. cDNA went through one round of normalization to Rot = 10.0 and subtraction to Rot = 229.0. Second strand cDNA was prepared with the primer adapter of sequence [5' GAGAGAGAGATTCTCGAGTTAATTAAATTAATCCCCCCCCCCCCC 3']. cDNA was cleaved with XhoI and BamHI. Vector: a modified pBluescript KS(+) after bulk excision from Lambda FLC I.

text: 4 days neonate

characteristic: dev-stage

text: Mus musculus

text: SalI

text: BamHI

text: male

characteristic: sex

text: adipose

text: RIKEN full-length enriched, 4 days neonate male adipose


text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: 4 days neonate

text: Mus musculus

text: SalI

text: BamHI

text: thymus

text: RIKEN full-length enriched, 4 days neonate thymus


text: The Institute of Physical and Chemical Research (RIKEN)

text: live

text: Generic

characteristic: description

text: 6 days neonate

text: Mus musculus

text: SalI

text: BamHI

text: medulla oblongata

text: RIKEN full-length enriched, 6 days neonate medulla oblongata


text: live

text: Generic

text: cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and Genome Science Laboratory in RIKEN. Division of Experimental Animal Research in Riken contributed to prepare mouse tissues. 1st strand cDNA was primed with a primer [5' GAGAGAGAGAAGGATCCAAGAGCTCTTTTTTTTTTTTTTTTVN 3'], cDNA was prepared by using trehalose thermo-activated reverse transcriptase and subsequently enriched for full-length by cap-trapper. cDNA went through one round of normalization to Rot = 20.0 and subtraction to Rot = 459.0. Second strand cDNA was prepared with the primer adapter of sequence [5' GAGAGAGAGATTCTCGAGTTAATTAAATTAATCCCCCCCCCCCCC 3']. cDNA was cleaved with XhoI and BamHI. Vector: a modified pBluescript KS(+) after bulk excision from Lambda FLC I.

text: 10 days neonate

characteristic: lab-host

text: Mus musculus

text: SalI

text: BamHI

text: medulla oblongata

text: RIKEN full-length enriched, 10 days neonate medulla oblongata


text: Philipps-University of Marburg

text: live

text: Generic

text: Homo sapiens

characteristic: organism

text: cartilage

text: Homo sapiens cartilage


text: College of Natural Resources and Life Science, Dong-A University

characteristic: INSDC center name

text: live

text: Generic

text: Pyrocoelia rufa

text: EcoRI

text: XhoI

text: whole body cDNA Library of Pyrocoelia rufa

text: pBluescript

characteristic: vector


text: The Samuel Roberts Noble Foundation

characteristic: INSDC first public

text: live

text: Generic

text: At the trifoliate stage, M. truncatula plants were transplanted to phosphate-free sand and grown for a further 30 days. During this period, they were fertilized twice weekly with 1/2 Hoaglands solutions containing 20uM potassium phosphate. cDNA was prepared from polyA+ enriched RNA. The cDNA was directionally ligated into the Unizap XR vector from Stratagene and packaged using Gigapack III Gold packaging extracts. Plasmids containing cDNA inserts were excised from the recombinant lambda-Zap phage using Ex-assist helper phage and propagated in XLOLR cells.

text: phosphate-starved

text: XLOLR

text: Medicago truncatula

text: EcoRI

text: XhoI

text: roots

text: MHRP-

characteristic: title

text: phagemid

text: pBluescript SK-


text: Institute of Basic Medical Sciences, PUMC & CAMS

characteristic: INSDC last update

text: live

text: Generic

text: erythroleukemia cell

characteristic: cell type

text: HEL

characteristic: cell-line

text: Homo sapiens

text: HEL cell line cDNA library


characteristic: External Id

text: live

text: Generic

text: cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and Genome Science Laboratory in RIKEN. Division of Experimental Animal Research in Riken contributed to prepare mouse tissues. 1st strand cDNA was primed with a primer [5' GAGAGAGAGAAGGATCCAAGAGCTCTTTTTTTTTTTTTTTTVN 3'], cDNA was prepared by using trehalose thermo-activated reverse transcriptase and subsequently enriched for full-length by cap-trapper. cDNA went through one round of normalization to Rot = 20.0 and subtraction to Rot = 479.0. Second strand cDNA was prepared with the primer adapter of sequence [5' GAGAGAGAGATTCTCGAGTTAATTAAATTAATCCCCCCCCCCCCC 3']. cDNA was cleaved with XhoI and BamHI. Vector: a modified pBluescript KS(+) after bulk excision from Lambda FLC I.

text: 0 day neonate

text: Mus musculus

text: SalI

text: BamHI

text: cerebellum

characteristic: tissue-type

text: RIKEN full-length enriched, 0 day neonate cerebellum


text: Institute of Basic Medical Science, Peking Union Medical College

text: live

characteristic: INSDC status

text: Generic

text: 2-4 months

text: Mus musculus

text: male and female

text: BALB/c

characteristic: strain

text: thymus

text: mouse thymus plasmid cDNA library


text: Texas Children's Cancer Center and Human Genome Sequencing Center at Baylor College of Medicine

text: live

text: Generic

text: myeloid cell

text: First strand cDNA was primed with an anchored XhoI-oligo(dT) primer [5'GGAGGACTCGAGCGGCCGCAGGAGGAG(T)VN 3'; V=A,C,G; N=A,C,G,T] and then dG tailed. Second strand was primed with a BamH1-dC primer [5'AGAGAGCTCGGATCCGCGGCCGCAATAATAATAAT(C) 3']. Double-stranded cDNA was then digested with BamH1 and XhoI and directionally cloned into the BamH1 and Sa1I sites of lambda pSB vector. Library went through one round of normalization. Library was constructed by Wei Yu at RIKEN of Japan (Carninci P, Westover A, Nishiyama Y, Ohsumi T, Itoh M, Nagaoka S, SasakiN, Okazaki Y, Muramatsu M, Schneider C, Hayashizaki Y, High efficiency selection of full-length cDNA by improved biotinylated cap trapper., DNA Res 4: 1, 61-6, Feb 28, 1997)

text: pediatric 6 years

characteristic: dev-stage

text: Homo sapiens

text: BamHI

text: EcoRI

text: male

characteristic: sex

text: leukopheresis

text: Pediatric acute myelogenous leukemia cell (FAB M1) Baylor-HGSC project=TCAA

text: lambda pSB


text: live

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: pre-B cell

text: pediatric 2 years

text: Homo sapiens

text: BamHI

text: EcoRI

text: male

text: leukopheresis

text: Pediatric pre-B cell acute lymphoblastic leukemia Baylor-HGSC project=TCBA

text: lambda pSB


text: Nanjing Medical University, Jiangsu Province, P.R.C.

text: live

text: Generic

text: These sequences were differentially expressed genes between Culex pipiens pallens resistant and susceptible strains to deltamethrin which were got by suppression subtractive hybridization. They were identified by cDNA microarray.

characteristic: description

text: Culex pipiens pallens

text: female

text: deltamethrin resistant and deltamethrin susceptible strains

text: Subtracted cDNA Library between deltamethrin resistant and susceptible strains


text: The Samuel Roberts Noble Foundation

text: live

text: Generic

text: Contains a mixture of entire plantlets harvested in a series of days-post-watering timepoints.

text: Pooled timepoints

text: Medicago truncatula

characteristic: organism

text: Plantlets

text: Drought

text: Plasmid

text: Lambda Zap


text: University of Arkansas

characteristic: INSDC center name

text: live

text: Generic

text: Library was produced from fully expanded M. truncatula leaves of plants fed upon by Spodoptera exigua (beet armyworm) for 24 hours. Systemic (undamaged leaves from injured plants) and wounded leaves were harvested and pooled.

text: mature

text: Medicago truncatula

text: local and systemic leaves

text: Insect herbivory

text: Plasmid

text: Lambda Zap


text: The Samuel Roberts Noble Foundation

text: live

text: Generic

text: At the trifoliate stage, M. truncatula plants were transplanted to phosphate-free sand and grown for a further 30 days. During this 30 day period, the plants were fertilized twice weekly with 1/2 Hoaglands solution containing only 20uM potassium phosphate. RNA was prepared from above ground tissues.

text: trifoliate

text: Medicago truncatula

text: leaf

text: Phosphate starved leaf

text: Plasmid

text: Lambda Zap

characteristic: vector


characteristic: INSDC first public

text: live

text: Generic

text: Cloned unidirectionally. Primer: Oligo dT. Average insert size 2 kb. Library constructed by Life Technologies.

text: DH10B (phage-resistant)

characteristic: lab-host

text: prostate

characteristic: organ

text: Rattus norvegicus

text: NotI

text: SalI

text: WU/HSD

text: phagemid (ampicillin resistant)


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

characteristic: INSDC last update

text: live

text: Generic

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCATGCATTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library constructed and normalized by Bento Soares and M.Fatima Bonaldo.

text: embryo, 10.5 dpc

text: DH10B (phage resistant)

text: Mus musculus

text: NotI

text: EcoRI

text: branchial arches

characteristic: title

text: phagemid (ampicillin resistant)


characteristic: External Id

text: live

text: Generic

text: cDNA made by oligo-dT priming. Library amplified by stretch PCR. Subtraction method: Bonaldo, et al., Genome Research 6:791. Library constructed by Dr. Archibald Perkins (Yale University).

text: adult

text: DH10B (phage-resistant)

text: Mus musculus

text: SalI

text: EagI

text: female

text: BALB/c

text: primary sorted bone marrow cells

text: Perkins LRH

text: plasmid (ampicillin resistant)


text: Washington University School of Medicine

text: live

text: Generic

text: Williams

characteristic: cultivar

text: This library was constructed from mRNA isolated from senescing leaf tissue of mature greenhouse grown plants of the cultivar Williams. Complementary DNA was synthesized from mRNA using a 3' anchored poly(dT) primer. EcoRI adapters were ligated to the blunt-ended cDNA fragments followed by digestion with EcoRI and HindIII. The cDNA fragments were directionally cloned into the EcoRI-HindIII restriction site of the pT7T3-Pac vector. The ligated cDNA fragments were transformed into DH10B host cells. This library was constructed by Dr. Randy Shoemaker.

text: Glycine max

text: EcoRI

text: HindIII

text: Senescing leaves, mature plant, greenhouse grown

characteristic: tissue-type

text: plasmid

text: pT7T3Pac (Pharmacia)


text: live

characteristic: INSDC status

text: Generic

text: Raiden

text: This cDNA library was constructed from mRNA isolated from 2 week old seedlings with the cotyledons removed at the time of harvest. The seedlings for the cultivar Raiden were grown in a growth chamber using germination paper. Complementary DNA was synthesized from mRNA using a primer consisting of a poly(dT) sequence with a XhoI restriction site. EcoRI adapters were ligated to the blunt-ended cDNA fragments followed by XhoI digestion. The cDNA fragments were directionally cloned into the EcoRI-XhoI restriction site of the pBluescript vector. The ligated cDNA fragments were transformed into DH10B host cells (Gibco BRL). This library was constructed by Dr. Randy Shoemaker.

text: Glycine max

text: EcoRI

text: XhoI

text: Whole seedling without cotyledons

text: plasmid

text: pBluescriptII SK+


text: University of Minnesota

text: live

text: Generic

text: Texsprout

text: DNA was digested with EcoRI, size separated by sucrose gradient centrifugation and the fraction between 500-3000 base pairs ligated into the vector using standard method.

text: Sprouts

characteristic: dev-stage

text: Vigna radiata

text: EcoRI

text: Hypocotyl and roots

text: RFLP sequences of mungbean, Vigna radiata

text: Plasmid

text: pcDNAII


text: The Institute of Physical and Chemical Research (RIKEN)

text: live

text: Generic

text: cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and Genome Science Laboratory in RIKEN. Division of Experimental Animal Research in Riken contributed to prepare mouse tissues. 1st strand cDNA was primed with a primer [5' GAGAGAGAGAAGGATCCAAGAGCTCTTTTTTTTTTTTTTTTVN 3'], cDNA was prepared by using trehalose thermo-activated reverse transcriptase and subsequently enriched for full-length by cap-trapper. Second strand cDNA was prepared with the primer adapter of sequence [5' GAGAGAGAGATTCTCGAGTTAATTAAATTAATCCCCCCCCCCCCC 3']. cDNA was cleaved with XhoI and BamHI. Vector: a modified pBluescript KS(+) after bulk excision from Lambda FLC I.

text: 7 days embryo

text: Mus musculus

text: SalI

text: BamHI

text: RIKEN full-length enriched, 7 days embryo


text: live

text: Generic

text: 12 days embryo

text: Mus musculus

text: SalI

text: BamHI

text: spinal cord

text: RIKEN full-length enriched, 12 days embryo spinal cord


text: Tongji University School of Medicine

text: live

text: Generic

text: It was prepared with suppression subtractive hybridization (SSH).

text: adult

text: Columba livia

text: brain

text: Pigeon optic tecta cDNA library generated with SSH methodology

text: Plasmid

text: pBluescript(sk-)


text: live

text: Generic

text: Williams

text: This cDNA library was constructed from mRNA isolated from hypocotyl and plumule tissues of seeds germinated for three days of the cultivar Williams. Complementary DNA was synthesized from mRNA using a primer consisting of a poly(dT) sequence with a NotI restriction site. EcoRI adapters were ligated to the blunt-ended cDNA fragments followed by digestion with EcoRI and NotI. The cDNA fragments were directionally cloned into the EcoRI-NotI restriction site of the pT7T3-Pac vector. The ligated cDNA fragments were transformed into DH10B host cells (Gibco BRL). This library was constructed by Dr. Randy Shoemaker.

text: Glycine max

text: EcoRI

text: NotI

text: Hypocotyl and Plumule, germinating seeds

text: plasmid

text: pT7T3Pac (Pharmacia)


text: live

text: Generic

text: mRNA made from colonic metastasis, cDNA made by oligo-dT priming. Non-directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 500 bp. Primary library. cDNA Library Preparation: David B. Krizman, Ph.D. REFERENCE: Krizman et al. (1996) Cancer Research 56:5380-5383.

text: colon

text: Homo sapiens

text: colonic adenocarcinoma metastasis

text: plasmid (ampicillin resistant)


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: mRNA made from leukoplakia, cDNA made by oligo-dT priming. Non-directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 500 bp. Primary library. cDNA Library Preparation: David B. Krizman, Ph.D. REFERENCE: Krizman et al. (1996) Cancer Research 56:5380-5383.

text: mouth

text: Homo sapiens

text: leukoplakia of the buccal mucosa

text: plasmid (ampicillin resistant)


text: US Department of Agriculture, Agriculture Research Service, Pacific West Area, Western Regional Research Center

text: live

text: Generic

text: Chinese Spring

text: Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Roots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

text: Five day old etiolated seedling

text: E. coli SOLR

text: Triticum aestivum

text: EcoRI

text: XhoI

text: Root

text: Wheat etiolated seedling root cDNA library

text: Phagemid

text: Lambda Uni-ZAP XR, excised phagemid


text: live

text: Generic

text: Islet cells

characteristic: cell type

text: cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and Genome Science Laboratory in RIKEN. Division of Experimental Animal Research in Riken contributed to prepare mouse tissues. 1st strand cDNA was primed with a primer [5' GAGAGAGAGAAGGATCCAAGAGCTCTTTTTTTTTTTTTTTTVN 3'], cDNA was prepared by using trehalose thermo-activated reverse transcriptase and subsequently enriched for full-length by cap-trapper. Second strand cDNA was prepared with the primer adapter of sequence [5' GAGAGAGAGATTCTCGAGTTAATTAAATTAATCCCCCCCCCCCCC 3']. cDNA was cleaved with XhoI and BamHI. Vector: a modified pBluescript KS(+) after bulk excision from Lambda FLC I.~Islet cells were provided by Hiroo Iwata, Institute for Frontier Medical Sciences, Kyoto University, Sakyo-ku, Kyoto, 606-8507 Japan, whose assistance we gratefully acknowledge.

text: adult

text: Mus musculus

text: SalI

text: BamHI

text: pancreas

text: RIKEN full-length enriched, adult pancreas Islet cells


text: live

text: Generic

characteristic: NCBI submission model

text: cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and Genome Science Laboratory in RIKEN. Division of Experimental Animal Research in Riken contributed to prepare mouse tissues. 1st strand cDNA was primed with a primer [5' GAGAGAGAGAGCGGCCGCAACTCGAGTTTTTTTTTTTTTTTTVN 3'], cDNA was prepared by using trehalose thermo-activated reverse transcriptase and subsequently enriched for full-length by cap-trapper. Second strand cDNA was prepared with the primer adapter of sequence [5' GAGAGAGAGAAGGATCCAAGAGCTCAATTAATTAATTAAACCCCCCCCCCC 3']. cDNA was cleaved with BamHI and XhoI. Vector: a modified pBluescript KS(+) after bulk excision from Lambda FLC I.

text: adult

text: Mus musculus

text: SalI

text: BamHI

text: male

characteristic: sex

text: heart

text: RIKEN full-length enriched, adult male heart


text: The Institute of Physical and Chemical Research (RIKEN)

text: live

text: Generic

characteristic: NCBI submission package

text: cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and Genome Science Laboratory in RIKEN. Division of Experimental Animal Research in Riken contributed to prepare mouse tissues. 1st strand cDNA was primed with a primer [5' GAGAGAGAGAGCGGCCGCAACTCGAGTTTTTTTTTTTTTTTTVN 3'], cDNA was prepared by using trehalose thermo-activated reverse transcriptase and subsequently enriched for full-length by cap-trapper. Second strand cDNA was prepared with the primer adapter of sequence [5' GAGAGAGAGAAGGATCCAAGAGCTCAATTAATTAATTAAACCCCCCCCCCC 3']. cDNA was cleaved with XhoI and SstI.

characteristic: description

text: 18 days embryo

text: SOLR

text: Mus musculus

text: XhoI

text: SstI

text: RIKEN full-length enriched, 18 days embryo


characteristic: INSDC center name

text: live

text: Generic

text: cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and Genome Science Laboratory in RIKEN. Division of Experimental Animal Research in Riken contributed to prepare mouse tissues. 1st strand cDNA was primed with a primer [5' GAGAGAGAGAGCGGCCGCAACTCGAGTTTTTTTTTTTTTTTTVN 3'], cDNA was prepared by using trehalose thermo-activated reverse transcriptase and subsequently enriched for full-length by cap-trapper. Second strand cDNA was prepared with the primer adapter of sequence [5' GAGAGAGAGATTCTCGAGTTAATTAAATTAATCCCCCCCCCCCCC 3']. cDNA was cleaved with BamHI and XhoI. Vector: a modified pBluescript KS(+) after bulk excision from Lambda FLC I.

text: 9 days embryo

text: Mus musculus

characteristic: organism

text: SalI

text: BamHI

text: RIKEN full-length enriched, 9 days embryo


text: Iwate University

characteristic: INSDC first public

text: live

text: Generic

text: Plants of Lotus japonicus Gifu B-129 were grown in a greenhouse condition. Sepal was removed from flower bud. The mRNA was isolated from flower bud of 0.7-1.0cm in length just before anther dehiscence.

text: SOLR

text: Lotus japonicus

text: ecotype Gifu B-129

characteristic: strain

text: mature flower bud

text: Lotus japonicus flower bud cDNA

text: pBlueScript SK(-)

characteristic: vector


text: Kyoto University

characteristic: INSDC last update

text: live

text: Generic

text: Marchantia polymorpha

text: male

text: E

text: sexual organ, antheridium, immature

text: Marchantia polymorpha strain E sexual organ, antheridium, immature


text: Institute of Basic Medical Science, Peking Union Medical College

text: live

text: Generic

text: from TaKaRa company

text: two months

text: Mus musculus

text: male and female

text: thymus

text: mouse thymus cDNA library

characteristic: title


characteristic: External Id

text: live

text: Generic

text: The library was made from poly-A RNA in the cloning vector lambda ZAP II. Clones to be sequenced were prepared by mass excision.

text: Mix of stamens of varying developmental stages from 8-week-old plants

characteristic: organ

text: Sorghum bicolor

text: XhoI

text: EcoRI

text: Anthers 1 (AN1)

text: Plasmid

text: pBluescript II from Lambda Zap II


text: The University of Georgia, Department of Plant Biology

text: live

text: Generic

text: Embryos germinated for 24 hr

text: Sorghum bicolor

text: XhoI

text: EcoRI

text: Embryo 1 (EM1)

text: Plasmid

text: pBluescript II from Lambda Zap II


text: live

text: Generic

text: mature plants were placed in a growth chamber for 15 days with 16 hr darkness and 8 hr light (flowering is induced by short-day conditions); 16 days after being returned to the greenhouse under natural long days during late April/early May, meristems were harvested The library was made from poly-A RNA in the cloning vector lambda ZAP II. Clones to be sequenced were prepared by mass excision.

text: Floral-induced meristems

text: Sorghum propinquum

text: XhoI

text: EcoRI

text: Floral-Induced Meristem 1 (FM1)

text: Plasmid

text: pBluescript II from Lambda Zap II


text: live

text: Generic

text: Mix of ovaries of varying immature stages from 8-week-old plants

text: Sorghum bicolor

text: XhoI

text: EcoRI

text: Ovary 1 (OV1)

text: Plasmid

text: pBluescript II from Lambda Zap II


text: live

text: Generic

text: Rhizomes

text: Sorghum halepense

text: XhoI

text: EcoRI

text: Rhizome1 (RHIZ1)

text: Plasmid

text: pBluescript II from Lambda Zap II


text: live

text: Generic

text: Two-week-old sorghum plants (BTX 623 cultivar) were infected with pathogen (isolate FRM42I of Colletotrichum graminicola, which is a sorghum isolate). RNA was prepared from infected leaves harvested from 45 seedlings 48 hours after inoculation. Note: young seedlings (2 weeks old) exhibit juvenile resistant reaction, which is an incompatible interaction. As they grow older (4 weeks or older), plants resume susceptiblity to anthracnose disease. The library was made from poly-A RNA in the cloning vector lambda ZAP II. Clones to be sequenced were prepared by mass excision. WARNING: While most or all ESTs are expected to derive from the host plant, no effort was made to eliminate ESTs deriving from the pathogen.

text: Anthracnose-infected leaves from two-week-old sorghum plants 48 hr after inoculation

text: Sorghum bicolor

text: XhoI

text: EcoRI

text: Pathogen induced 1 (PI1)

text: Plasmid

text: pBluescript II from Lambda Zap II


text: University of Goettingen,

text: live

text: Generic

text: germ cells

characteristic: cell-line

text: cDNA isolated by using a suppression subtractive hybridization (SSH) between the immortalized spermatogonia-derived cell line GC-1spg (Exp Cell Res 201,417-435 (1992)) and the spermatocyte-derived cell line GC-4 spc (Biol Reprod. in press)

text: Mus musculus

text: SstII

text: Pst I

text: male

text: BALB/c

text: testis

characteristic: tissue-type

text: GC-1 spg cDNA library

text: Plasmid

text: pGEM-T (Promega)


text: Institute for Cancer Research, The Norwegian Radium Hospital

text: live

characteristic: INSDC status

text: Generic

text: Homo sapiens

text: Liposarcoma

text: RACE product


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: cDNA made by oligo-dT priming. Directionally cloned into EcoRI/XhoI sites using the following 5' adaptor: GGCACGAG(G). Library constructed by Ling Hong in the laboratory of Gerald M. Rubin (University of California, Berkeley) using ZAP-cDNA synthesis kit (Stratagene) and Superscript II RT (Life Technologies).

text: DH10B (phage-resistant)

characteristic: lab-host

text: uterus

text: Homo sapiens

text: XhoI

text: EcoRI

text: endometrium, adenocarcinoma cell line

text: plasmid (chloramphenicol resistant, grown at 30C)


text: Universite Libre de Bruxelles ULB

text: live

text: Generic

characteristic: NCBI submission model

text: promastigote

characteristic: dev-stage

text: Leishmania infantum

text: page


text: Universite Libre de Bruxelles ULB

text: live

text: Generic

characteristic: NCBI submission package

text: promastigote

text: Leishmania infantum


text: University of Dundee, Ninewells Hospital

text: live

text: Generic

text: primary fibroblasts

characteristic: cell type

text: newborn

text: Mesocricetus auratus

text: kkin

text: Mesocricetus auratus kkin newborn


text: Dept. of Plant and Soil Science, Montana State University

characteristic: INSDC center name

text: live

text: Generic

text: Cheyenne

characteristic: cultivar

text: Wheat Endosperm Library constructed in Lambda ZAPII with 8-mer adapter.

characteristic: description

text: 5-30 days post anthesis

text: Triticum aestivum

text: endosperm

text: ITEC WHE Wheat Endosperm Library

text: Phagemid

text: Lambda ZAPII


text: Div. of Plant Industry, CSIRO

characteristic: INSDC first public

text: live

text: Generic

text: Wyuna

text: Plants grown in Phytotron with 18C/13C (day/night) 16 hour light. M13 Reverse sequencing primer used. 1.0 Kbp average insert size.

text: 8-12 days post anthesis

text: Escherichia coli SOLR

text: Triticum aestivum

characteristic: organism

text: XhoI

text: EcoRI

text: endosperm

text: ITEC CSB Wheat Endosperm Library

text: Phagemid

text: Lambda Zap/Bluescript


text: Cereal Research Centre, Agriculture & Agri-Foods Canada

characteristic: INSDC last update

text: live

text: Generic

text: Thatcher Lr1

text: 1.0 Kbp average insert size.

text: after 24 hour challenge with LR-AVR1

text: Triticum aestivum

text: young leaf

text: ITEC SCL Wheat Leaf Library

text: Phagemid

text: Lambda ZAP

characteristic: vector


characteristic: External Id

text: Institute for Plant Genetics & Crop Plant Research

text: live

text: Generic

text: etiolated

text: Hordeum vulgare

text: leaf/culm

text: ITEC MCG Barley Leaf/Culm Library

characteristic: title


text: TU Munchen, Lehrstuhl fur Pflanzenbau und Pflanzenzuchtung

text: live

text: Generic

text: Barke

text: 850 bp average insert size.

text: 14 day old

text: Hordeum vulgare subsp. vulgare

text: leaf

text: ITEC HWM Barley Leaf Library

text: Plasmid

text: pBluescriptSK(-)


text: Centre for Plant Conservation Genetics, Southern Cross University

text: live

text: Generic

text: Wyuna

text: Triticum aestivum

text: endosperm

text: ITEC SCU Wheat Endosperm Library

text: Bluescript II SK(-)


text: Centre de Recherches Agronomiques, Departement de Biotechnologie

text: live

text: Generic

text: Odeon

text: 0.3-2.0 Kbp average insert size.

text: seedling, unstressed

text: Triticum aestivum

text: root

text: ITEC GBX Wheat Root Library

text: Plasmid


text: Inst. of Crop Germplasm Resources

text: live

text: Generic

text: Powdery Mildew Resistant line

text: Primer used: 5'-TCCGAGATCTGGACGAGC-3' 500 bp average insert size.

text: seedling, challenged with powdery mildew strain

text: Triticum aestivum

text: SfiI

text: leaf

text: ITEC CNW Wheat Powdery Mildew Resistant Library

text: Phage

text: Lambda TriplEX


text: Special Research Centre, Basic and Applied Plant Molecular Biology

text: live

text: Generic

text: Chinese Spring

text: Library constructed in pSport 1. Directionally cloned using the Superscript Plasmid System for cDNA synthesis and plasmid cloning. M13 Reverse sequencing primer used to obtain 5' sequence data. 1.4 Kbp average insert size.

text: meiotic stage no later than metaphase I

text: Triticum aestivum

text: SalI

text: excised florets

text: ITEC AWB Wheat Meiotic Stage Library

text: Plasmid

text: pSport 1 (Life Technologies cat. no. 18248-013)


text: Agricultural Biotechnology R&D, Life Technologies, Inc.

text: live

text: Generic

text: Catoctin

text: fully developed after germination

text: Triticum aestivum

text: leaf

text: ITEC JJL Wheat Leaf Library

text: pSport-P


text: Kyoto Prefectural University

text: live

text: Generic

text: Norin 26

text: M13 Reverse sequencing primer used. 1.2 Kbp average insert size.

text: Feekes' scale 6-7

text: Triticum aestivum

text: EcoRI

text: XhoI

text: young spikelets

text: ITEC MUG Wheat Spikelet Library

text: Plasmid

text: pBluescript SK(-)


text: Istituto Sperimentale per la Cerealicoltura

text: live

text: Generic

text: Nure

text: 0.7-2.0 Kbp average insert size.

text: first leaf, cold stress

text: Hordeum vulgare subsp. vulgare

text: leaf

text: ITEC ISC Barley Leaf Library

text: UNI-ZAP XR


text: Zeneca Wheat Improvement Centre, Norwich Research Park

text: live

text: Generic

text: Novosibirskaya 67

text: M13 Reverse sequencing primer used for 5' end of clone.

text: Triticum aestivum

text: root

text: ITEC WWR Wheat Root Library


text: live

text: Generic

text: Novosibirskaya 67

text: Triticum aestivum

text: scutellum callus

text: ITEC WWS Wheat Scutellum Library


text: Applied Biotechnology Center, CIMMYT

text: live

text: Generic

text: Atlas

text: 0.5-1.5 Kbp average insert size.

text: 8 day old

text: Triticum aestivum

text: root

text: ITEC MWL Wheat Root Library

text: Plasmid

text: pYES2 (Invitrogen)


text: The Institute of Physical and Chemical Research (RIKEN)

text: live

text: Generic

text: cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and Genome Science Laboratory in RIKEN. Division of Experimental Animal Research in Riken contributed to prepare mouse tissues. 1st strand cDNA was primed with a primer [5' GAGAGAGAGAGCGGCCGCAACTCGAGTTTTTTTTTTTTTTTTVN 3'], cDNA was prepared by using trehalose thermo-activated reverse transcriptase and subsequently enriched for full-length by cap-trapper. Second strand cDNA was prepared with the primer adapter of sequence [5' GAGAGAGAGATTCTCGAGTTAATTAAATTAATCCCCCCCCCCCCC 3']. cDNA was cleaved with BamHI and XhoI. Vector: a modified pBluescript KS(+) after bulk excision from Lambda FLC I.

text: 12 days embryo

text: Mus musculus

text: SalI

text: BamHI

text: spinal ganglion

text: RIKEN full-length enriched, 12 days embryo spinal ganglion


text: live

text: Generic

text: 12 days embryo

text: Mus musculus

text: SalI

text: BamHI

text: eyeball

text: RIKEN full-length enriched, 12 days embryo eyeball


text: live

text: Generic

text: 13 days embryo

text: Mus musculus

text: SalI

text: BamHI

text: heart

text: RIKEN full-length enriched, 13 days embryo heart


text: US Department of Agriculture, Agriculture Research Service, Pacific West Area, Western Regional Research Center

text: live

text: Generic

text: Chinese Spring

text: Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Shoots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

text: Five day old seedling

text: E. coli SOLR

text: Triticum aestivum

text: EcoRI

text: XhoI

text: Etiolated shoot

text: Wheat unstressed seedling shoot cDNA library

text: Phagemid

text: Lambda Uni-ZAP XR, excised phagemid


text: live

text: Generic

text: Cheyenne

text: Seeds collected, endosperm isolated, and RNA prepared by Susan Altenbach. Library constructed by Stratagene, Inc. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab.

text: 5 to 30 days post anthesis seed

text: E. coli SOLR

text: Triticum aestivum

text: EcoRI

text: Endosperm

text: Wheat endosperm cDNA library

text: Phagemid

text: Lambda ZAP II, excised phagemid


text: live

text: Generic

text: 13 days embryo

text: Mus musculus

text: SalI

text: BamHI

text: lung

text: RIKEN full-length enriched, 13 days embryo lung


text: live

text: Generic

text: 13 days embryo

text: Mus musculus

text: SalI

text: BamHI

text: stomach

text: RIKEN full-length enriched, 13 days embryo stomach


text: Institute of Basic Medical Science, Peking Union Medical College

text: live

text: Generic

text: Homo sapiens

text: bone marrow

text: human bone marrow cDNA library


text: Cereals Group, John Innes Centre

text: live

text: Generic

text: Soleil

text: 1:1 mix 10:20 days post anthesis

text: Triticum aestivum

text: endosperm

text: ITEC PSR Wheat Endosperm Library

text: Phagemid

text: Lambda ZAP II (Stratagene)


text: Cereals Group, John Innes Centre

text: live

text: Generic

text: Novosibirskaya 67

text: 3:1 mix 10:20 days post anthesis

text: Triticum aestivum

text: pericarp/testa

text: ITEC PSR Wheat Pericarp/Testa Library

text: Phagemid

text: Lambda ZAP II (Stratagene)


text: Cereals Group, John Innes Centre

text: live

text: Generic

text: Forester

text: immature; developing

text: Hordeum vulgare subsp. vulgare

text: inflorescence

text: ITEC PSR Barley Inflorescence Library

text: Phagemid

text: Lambda ZAP II (Stratagene)


text: INRA, Unite de Biochimie et Biologie Moleculaire des Cereales

text: live

text: Generic

text: Siliana

text: T7 primers used. See pSPORT1 polylinker site. 0.3-2.0 Kbp average insert size.

text: 3-day-old seedling, water-stressed

text: Triticum turgidum subsp. durum

text: durum

characteristic: sub species

text: root

text: ITEC MTD Durum Wheat Root Library

text: Plasmid


text: Plant Biotechnology Institute, National Research Council of Canada

text: live

text: Generic

text: Triticum aestivum

text: ITEC TAS Wheat cDNA Library


text: Plant Breeding Institute, University of Sydney

text: live

text: Generic

text: Triticum aestivum

text: ITEC SUN Wheat cDNA Library


text: Institute for Plant Biology, University of Zurich

text: live

text: Generic

text: Franka

text: 5' Triplex sequencing primer 5'-TCCGAGATCTGGACGAGC-3'. Other restriction sites: XbaI, HindIII ,NotI 800bp average insert size.

text: 10 day old seedling

text: Hordeum vulgare subsp. vulgare

text: EcoRI

text: SacI

text: leaf epidermis

characteristic: tissue-type

text: ITEC SFR Barley Leaf Epidermis Library

text: Phage

text: Lambda TriplEx2


text: Dept. of Agronomy, University of Missouri

text: live

characteristic: INSDC status

text: Generic

text: Blanco

text: yound roots, Aluminum stressed

text: Secale cereale

text: root tip

text: ITEC SCC Rye Root Library


text: Centro de Pesquisas Rene Rachou - FIOCRUZ

text: live

text: Generic

text: mRNA was extracted from in vitro cultured lung stage worms and the library was constructed and excised according to the manufacturer's instructions.

text: Lung

text: Schistosoma mansoni

text: EcoRI

text: XhoI

text: Mixed

characteristic: sex

text: LE

characteristic: strain

text: Lung stage cDNA expression library in Lambda ZAPII

text: Uni-Zap XR vector, Stratagene (pBluescript SK)


text: live

text: Generic

characteristic: NCBI submission model

text: mRNA was extracted from adult worms and amplified with the SMART kit (Clonetech) protocol according to the manufacturer's instructions. PCR products were cloned using the TOPO cloning kit (Invitrogen) according to the manufacturer's instructions.

text: Adult worm

characteristic: dev-stage

text: Schistosoma mansoni

text: EcoRI

text: EcoRI

text: Mixed

text: LE

text: Adult worm stage cDNA amplified with the SMART PCR kit from Clonetech

text: pCR2.1TOPO (Invitrogen)


text: live

text: Generic

characteristic: NCBI submission package

text: Adult worm

text: Schistosoma mansoni

text: EcoRI

text: EcoRI

text: Mixed

text: LE

text: Adult worm stage lambda GT10 library amplified with gene specific primers

text: pCR2.1TOPO (Invitrogen)


characteristic: INSDC center name

text: live

text: Generic

text: mRNA was extracted from cercariae and amplified with the SMART kit (Clonetech) protocol according to the manufacturer's instructions. PCR products were cloned using the TOPO cloning kit (Invitrogen) according to the manufacturer's instructions.

text: Cercaria

text: Schistosoma mansoni

text: EcoRI

text: EcoRI

text: Mixed

text: LE

text: Cercaria cDNA obtained by using the SMART PCR kit from Clonetech

text: pCR2.1TOPO (Invitrogen)


text: Clemson University

text: live

text: Generic

characteristic: cultivar

text: Fruit were harvested at the breaker stage (first sign of lycopene accumulation on the blossom end of the fruit). Fruit were cut in half and the seeds and locules were discarded prior to freezing the pericarp.

text: breaker

text: SOLR

characteristic: lab-host

text: Solanum lycopersicum

text: XhoI

text: Pericarp

text: tomato breaker fruit, TIGR

text: phagemid

text: pBluescriptSKmCUadapt


text: Agriculture and Agri-food Canada

characteristic: INSDC first public

text: live

text: Generic

text: This probe has been used for mapping studies for species of the Triticeae and is available from the GrainGenes Probe Repository ( http://wheat.pw.usda.gov )

characteristic: description

text: Hordeum vulgare

text: leaf

text: ITEC BCD Barley cDNA Library

text: Plasmid

text: pBluescriptSK(-)


text: Agriculture and Agri-food Canada

characteristic: INSDC last update

text: live

text: Generic

text: Avena sativa

characteristic: organism

text: leaf

text: ITEC CDO Oat cDNA Library

text: Plasmid

text: pBluescriptSK(-)


characteristic: External Id

text: Beijing Institute of Geriatrics &Beijing Hospital

text: live

text: Generic

text: Endothelial cells

characteristic: cell type

text: Human Lambda Zap Express Library

text: Homo sapiens

text: Venae

text: Human umbilical vein cord


text: Duke University

text: live

text: Generic

text: Homo sapiens

text: Trabecular meshwork

characteristic: title


text: Clemson University

text: live

text: Generic

text: Fruit were tagged at 5 dpa (0.5 cm) and harvested at 7 day intervals through 35 dpa. Equal masses of tissue from each stage were combined (including seeds and locules) prior to mRNA isolation.

text: immature green (5-35 days post-anthesis)

text: SOLR

text: Solanum lycopersicum

text: fruit

characteristic: tissue-type

text: tomato developing/immature green fruit

text: phagemid

text: pBluescriptSKmCUadapt

characteristic: vector


text: National Institute for Occupational Safety and Health

text: live

characteristic: INSDC status

text: Generic

text: alveolar macrophage

text: Homo sapiens

text: Differentiation Display RT-PCR of human alveolar macrophage mRNA


text: US Department of Agriculture, Agriculture Research Service, Pacific West Area, Western Regional Research Center

text: live

text: Generic

text: Chinese Spring

text: Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Roots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. The cDNA clones were in vivo excised to give pBluescript phagemids before normalization was carried out. The mass excision of phagemid library and normalization were done in HT Nguyen lab by D. Zhang at Texas Tech Univeristy. Normalization protocol used was that of Soares. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

text: Five day old etiolated seedling

text: E. coli DH10B

text: Triticum aestivum

text: EcoRI

text: XhoI

text: Root

text: Wheat etiolated seedling root normalized cDNA library

text: Phagemid

text: Lambda Uni-ZAP XR, excised phagemid pBluescript SK


text: The Institute of Physical and Chemical Research (RIKEN)

text: live

text: Generic

text: cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and Genome Science Laboratory in RIKEN. Division of Experimental Animal Research in Riken contributed to prepare mouse tissues. 1st strand cDNA was primed with a primer [5' GAGAGAGAGAGCGGCCGCAACTCGAGTTTTTTTTTTTTTTTTVN 3'], cDNA was prepared by using trehalose thermo-activated reverse transcriptase and subsequently enriched for full-length by cap-trapper. Second strand cDNA was prepared with the primer adapter of sequence [5' GAGAGAGAGATTCTCGAGTTAATTAAATTAATCCCCCCCCCCCCC 3']. cDNA was cleaved with BamHI and XhoI. Vector: a modified pBluescript KS(+) after bulk excision from Lambda FLC I.

text: 0 day neonate

text: Mus musculus

text: SalI

text: BamHI

text: kidney

text: RIKEN full-length enriched, 0 day neonate kidney


text: live

text: Generic

text: 10 days lactation, adult

text: Mus musculus

text: SalI

text: BamHI

text: female

characteristic: sex

text: mammary gland

text: RIKEN full-length enriched, 10 days lactation, adult female mammary gland


text: live

text: Generic

characteristic: NCBI submission model

text: 16 days neonate

text: Mus musculus

text: SalI

text: BamHI

text: heart

text: RIKEN full-length enriched, 16 days neonate heart


text: North Carolina State University

text: live

text: Generic

text: Compression

text: The library is from early (spring) wood, taken from three six-year old trees (three different genotypes), in the juvenile phase. These trees were induced to form compression wood by bending to a 45 degree angle and tying them to the ground. Differentiating xylem was harvested from the bottoms of the inclined stems, and a mixture of all three genotypes was used for the library. oligo-dT primed cDNA was directionally cloned into the EcoRI-XhoI BlueScript SK vector arms. NOTE: The sequences contain a 'cDNA adapter' between the EcoRI site and the start of the EST. The adapter sequence is 'AATTCGGCACGAG'.

text: Juvenile

characteristic: dev-stage

text: Pinus taeda

text: Eco RI

text: XhoI

text: Coastal plain loblolly pine from North Carolina

characteristic: strain

text: Xylem

text: NXCI (Nsf Xylem Compression wood Inclined)

text: BlueScript SK


text: Washington University School of Medicine

characteristic: INSDC center name

text: live

text: Generic

characteristic: NCBI submission package

text: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACGAATCTGAAGTGGGAGCGGCCGCCCTTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Eco RI adaptors [5' AATTCACTAGTTTT 3' and 5' AAAACTAGTG 3'], digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library constructed by Bob Barstead.

text: adult

text: DH10B (phage resistant)

text: testis

characteristic: organ

text: Mus musculus

text: EcoRI

text: NotI

text: male

text: pachytene spermatocyte

text: Barstead mouse pachytene MPLRB15

text: phagemid (ampicillin resistant)


characteristic: INSDC first public

text: live

text: Generic

text: 0 day neonate

characteristic: lab-host

text: Mus musculus

text: SalI

text: BamHI

text: lung

text: RIKEN full-length enriched, 0 day neonate lung


characteristic: INSDC last update

text: live

text: Generic

characteristic: description

text: 0 day neonate

text: Mus musculus

text: SalI

text: BamHI

text: eyeball

text: RIKEN full-length enriched, 0 day neonate eyeball


characteristic: External Id

text: live

text: Generic

text: 2 days pregnant adult

text: Mus musculus

characteristic: organism

text: SalI

text: BamHI

text: female

text: oviduct

text: RIKEN full-length enriched, 2 days pregnant adult female oviduct


text: live

text: Generic

text: 2 days pregnant adult

text: Mus musculus

text: SalI

text: BamHI

text: female

text: ovary

text: RIKEN full-length enriched, 2 days pregnant adult female ovary

characteristic: title


text: live

text: Generic

text: 10 days neonate

text: Mus musculus

text: SalI

text: BamHI

text: olfactory brain

characteristic: tissue-type

text: RIKEN full-length enriched, 10 days neonate olfactory brain


text: Auburn University

text: live

text: Generic

text: Head kidney

text: Ictalurus punctatus

text: NotI

text: SalI

text: Head kidney cDNA library

text: Phagemid

characteristic: vector


text: US Department of Agriculture, Agriculture Research Service, Pacific West Area, Western Regional Research Center

text: live

characteristic: INSDC status

text: Generic

text: Chinese Spring

characteristic: cultivar

text: Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Five-day old seedlings were incubated for one day at 90% RH. After removing endosperm, seedlings were transferred to desiccator jar containing saturated MgSO4 at room temperature for 24 hr. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

text: Five day old seedling

text: E. coli SOLR

text: Triticum aestivum

text: EcoRI

text: XhoI

text: Seedling without endosperm

text: Wheat drought-stressed seedling cDNA library

text: Phagemid

text: Lambda Uni-ZAP XR, excised phagemid


text: live

text: Generic

characteristic: NCBI submission model

text: Williams

text: This cDNA library was constructed from mRNA isolated from etiolated hypocotyl tissue of 9-10 day old seedlings of the cultivar Williams. Complementary DNA was synthesized from mRNA using a primer consisting of a poly(dT) primer with a XhoI restriction site. EcoRI adapters were ligated to the blunt-ended cDNA fragments followed by digestion with EcoRI and XhoI. The cDNA fragments were directionally cloned into the EcoRI-XhoI restriction site of the pBluescript vector. The ligated cDNA fragments were transformed into DH10B host cells (Gibco BRL). This library was constructed by Dr. Randy Shoemaker.

text: Glycine max

text: EcoRI

text: XhoI

text: Hypocotyl, 9-10 day old etiolated seedlings

text: plasmid

text: pBluescriptII SK+


text: USDA, ARS, Beltsville Agricultural Research Center

text: live

text: Generic

text: Library made from pooled mRNA isolated from mammary tissues at eight physiological, developmental, and disease states.

text: Bos taurus

text: NotI

text: SalI

text: pooled

text: BARC 5BOV

text: plasmid

text: pCMV SPORT6


text: SUNY College of Environmental Science & Forestry

text: live

text: Generic

text: 2 years+

characteristic: dev-stage

text: Castanea dentata

text: stem

text: American Chestnut subtracted cDNA stem library ACEST001


text: live

text: Generic

text: 2 years+

text: Castanea dentata

text: stem

text: American Chestnut cDNA stem library ACEST002


text: live

text: Generic

text: Chinese Spring

text: Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Roots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. The cDNA clones were in vivo excised to give pBluescript phagemids before normalization was carried out. The mass excision of phagemid library and normalization were done in HT Nguyen lab by D. Zhang at Texas Tech Univeristy. Normalization protocol used was that of Soares'. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

text: Five day old seedling

text: E. coli DH10B

text: Triticum aestivum

text: EcoRI

text: XhoI

text: Etiolated shoot

text: Wheat unstressed seedling shoot normalized cDNA library

text: Phagemid

text: Lambda Uni-ZAP XR, excised phagemid pBluescript SK


text: live

text: Generic

text: Chinese Spring

text: Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Five-day old seedlings were transferred to 5 C cold room and kept for 48 hr. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

text: Five-day old seedling

text: E. coli SOLR

text: Triticum aestivum

text: EcoRI

text: XhoI

text: Seedling

text: Wheat cold-stressed seedling cDNA library

text: Phagemid

text: Lambda Uni-ZAP XR, excised phagemid


text: live

text: Generic

text: Takifugu rubripes

text: Japanese pufferfish (Fugu rubripes) cDNA


text: Kyoto University

text: live

text: Generic

text: fetus

text: Bos taurus

text: Cloned bovine fetus cDNA


text: National Taiwan University Medical School

text: live

text: Generic

text: lymphoma line

characteristic: cell type

characteristic: cell-line

text: Homo sapiens

text: Retinoic acid-induced differential display cDNA library

text: Plasmid

text: pGEM T


text: Cancer Institute, Chinese Academy of Medical Sciences

characteristic: INSDC center name

text: live

text: Generic

characteristic: NCBI submission package

text: Homo sapiens

text: esophageal squamous cell carcinoma

text: suppression subtractive hybridization library SEC1


text: Stanford University

characteristic: INSDC first public

text: live

text: Generic

text: adenocarcinoma

text: fragments generated using suppression subtractive hybridization (SSH) PCR with polyA+RNA from MCF7 cells

text: Homo sapiens

text: EcoR I

text: EcoR I

text: female

characteristic: sex

text: breast

text: estradiol-responsive cDNAs from MCF7 cell line (Homo sapiens breast adenocarcinoma)

text: Plasmid

text: pCDNA 2.1 TA cloning vector


characteristic: INSDC last update

text: live

text: Generic

text: The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised at the University of California, Davis (V. Echenique, B. Stamova, J. Dubcovsky). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

text: Three weeks-old plants

text: E. coli XLOLR

characteristic: lab-host

text: Triticum monococcum

text: EcoRI

text: XhoI

text: Vegetative shoot apex

text: Triticum monococcum shoot apex cDNA library

text: Phagemid

text: Lambda pBK-CMV (Lambda Zap Express), excised phagemid


characteristic: External Id

text: US Department of Agriculture, Agriculture Research Service, Pacific West Area, Western Regional Research Center

text: live

text: Generic

text: Blanco

text: Plants were grown in the greenhouse. Anthers were harvested and pooled from early meiosis to late meiosis. The tissue, total RNA, and poly(A) RNA were prepared (Butler, Ross and Gustafson) at University of Missouri, Columbia. A cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

characteristic: description

text: Adult plant before anthesis

text: E. coli SOLR

text: Secale cereale

text: EcoRI

text: XhoI

text: Anther

text: Secale cereale anther cDNA library

text: Phagemid

text: Lambda Uni-ZAP XR, excised phagemid


text: National Institute for Basic Biology

text: live

text: Generic

text: adult

text: Oryzias latipes

characteristic: organism

text: HNI

characteristic: strain

text: whole body

text: Sugano Kawakami 5' enriched cDNA library (OLa)

characteristic: title


text: Genoscope - Centre National de Sequencage

text: live

characteristic: INSDC status

text: Generic

text: Jemalong

text: Plants were grown in an aeroponic chamber for 14 days on nitrogen-rich medium followed by 3 days on N-free medium. RNA was extracted from root tips (1-3 cm). cDNA was prepared from polyA+ enriched RNA. The cDNA was directionally ligated into Uni-zapXR vector from Stratagene and packaged using Gigapack Gold packaging extracts. Plasmids containing cDNA inserts were mass-excised from phage stocks using ExAssit helper phage and propagated in SOLR cells. Clone ordering and sequencing was performed by the Centre National de Sequencage (Genoscope, Evry, France).

text: harvested after 3 days of N-starvation

text: Medicago truncatula

text: EcoRI

text: XhoI

text: root tips

characteristic: tissue-type

text: MtBA

text: pBluescript pSK

characteristic: vector


text: live

text: Generic

characteristic: NCBI submission model

text: Jemalong

characteristic: cultivar

text: Plants were grown in an aeroponic chamber on nitrogen-rich medium for 21 days. Three days before inoculation with Sinorhizobium meliloti, the medium was replaced by N-free medium. Root nodules (+ short adjacent root segments) were harvested 4 days post inoculation. cDNA was prepared from polyA+ enriched RNA. The cDNA was directionally ligated into Uni-zap XR vector from Stratagene and packaged using Gigapack Gold packaging extracts. Plasmids containing cDNA inserts were mass-excised from phage stocks using ExAssit helper phage and propagated in SOLR cells. Clone ordering and sequencing was performed by the Centre National de Sequencage (Genoscope, Evry, France).

text: harvested 4 days post inoculation with Sinorhizobium meliloti

characteristic: dev-stage

text: Medicago truncatula

text: EcoRI

text: XhoI

text: symbiotic root nodules

text: MtBB

text: pBluescript pSK


characteristic: INSDC center name

text: live

text: Generic

text: Jemalong

text: M. truncatula sterilised seeds were germinated for 72h at 25 C, before transplanting into a 1/3 Epoisses soil : 2/3 calcined Terragreen mix in the presence of onion root fragments colonized by the arbuscular mycorrhizal fungus Glomus intraradices (Schenck & Smith, isolate LPA8). The plants were watered every day and twice a week with a modified nutrient Long Ashton solution without phosphate but with a high level of nitrate. After 3 weeks RNA was extracted from whole root systems. cDNA was prepared from polyA+ enriched RNA. The cDNA was directionally ligated into Uni-zap XR vector from Stratagene and packaged using Gigapack Gold packaging extracts. Plasmids containing cDNA inserts were mass-excised from phage stocks using ExAssit helper phage and propagated in SOLR cells. Clone ordering and sequencing was performed by the Centre National de Sequencage (Genoscope, Evry, France). Note : EST may be of fungal origin.

text: harvested 3 weeks post inoculation with Glomus intraradices

text: Medicago truncatula

text: EcoRI

text: XhoI

text: arbuscular mycorrhiza

text: MtBC

text: pBluescript pSK


text: University of Bonn

text: live

text: Generic

characteristic: NCBI submission package

text: cDNA library was constructed with the Clontech cDNA library construction kit

text: oocyte

text: Bos taurus

text: bovine oocyte cDNA library


text: Morehouse School of Medicine

characteristic: INSDC first public

text: live

text: Generic

text: First-strand cDNA was synthesized from SCN total RNA of rat brain with reverse transcriptase, and then several clones suspected being related to circadian rhythm were screened by PCR-based differential display technique.

text: Brain

characteristic: organ

text: Rattus norvegicus

text: EcoRI

text: EcoRI

text: Sprague-Dawley

text: SCN

text: Rat Brain SCN cDNA library

text: plasmid

text: pCR 3.1


text: Martin-Luther-University Halle-Wittenberg

characteristic: INSDC last update

text: live

text: Generic

text: neonatal

text: Sus scrofa

text: M. biceps femoris

text: Sus scrofa M. biceps femoris neonatal


text: Friedrich-Alexander-Universitaet Erlangen-Nuernberg

text: live

text: Generic

text: CD4 positive T-lymphocytes

characteristic: cell type

text: adult T-cell leukemia cell line JuaW

characteristic: cell-line

text: Homo sapiens

text: Homo sapiens adult T-cell leukemia cell line JuaW


characteristic: External Id

text: live

text: Generic

text: CD4 positive T-lymphocytes

text: HTLV-1 transformed T cell line C91-PL

text: Homo sapiens

text: Homo sapiens HTLV-1 transformed T cell line C91-PL


text: Max Planck Institut Fuer Molekulare Genetik

text: live

text: Generic

text: 9 and 12 pc embryo

text: Mus musculus

characteristic: organism

text: embryo

text: ICRFp 522 and 523


text: live

text: Generic

text: Chinese Spring

text: Two-week old seedling were heat treated at 37 C for 2 hr and cyclic heat treated at 37 C for 2 hr for two days at Texas Tech University (D. Zhang, H. Nguyen lab). The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

characteristic: description

text: Two-week old seedling

text: E. coli SOLR

characteristic: lab-host

text: Triticum aestivum

text: EcoRI

text: XhoI

text: Leaf

text: Wheat heat-stressed seedling cDNA library

text: Phagemid

text: Lambda Uni-ZAP XR, excised phagemid


text: US Department of Agriculture, Agriculture Research Service, Pacific West Area, Western Regional Research Center

text: live

characteristic: INSDC status

text: Generic

text: Chinese Spring

text: Plants were grown in the greenhouse. Whole spike with awns trimmed, white, green and yellow anther were collected and total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

text: Adult plant

text: E. coli SOLR

text: Triticum aestivum

text: EcoRI

text: XhoI

text: Spike before anthesis

text: Wheat pre-anthesis spike cDNA library

characteristic: title

text: Phagemid

text: Lambda Uni-ZAP XR, excised phagemid


text: Centro de Pesquisas Rene Rachou - FIOCRUZ

text: live

text: Generic

text: Blunt-ended cloning (SureClone Kit). CaCl2 transformation of competent XL1-blue cells

text: Adult worm

text: Schistosoma mansoni

text: Male

characteristic: sex

text: LE

characteristic: strain

text: Male adult worm cDNA expression randomly amplified mini-library in pUC18

characteristic: vector


text: live

text: Generic

characteristic: NCBI submission model

text: Day seven schistosomula

characteristic: dev-stage

text: Schistosoma mansoni

text: Mix

text: LE

text: Day 7 schistosomula cDNA expression randomly amplified mini-library in pUC18


text: Shirakawa Institute of Animal Genetics

characteristic: INSDC center name

text: live

text: Generic

characteristic: NCBI submission package

text: Poly A was deleted from a Not1 site

text: fetus

text: Bos taurus

text: brain

characteristic: tissue-type

text: Bos taurus brain fetus


text: Shirakawa Institute of Animal Genetics

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Poly A was deleted from a Not1 site

text: fetus

text: Bos taurus

text: cartilage

text: Bos taurus cartilage fetus


text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: mRNA made from tongue epithelium, cDNA made by oligo-dT priming. Non-directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 500 bp. Primary library. cDNA Library Preparation: David B. Krizman, Ph.D. REFERENCE: Krizman et al. (1996) Cancer Research 56:5380-5383.

text: tongue

characteristic: organ

text: Homo sapiens

text: hyperplasia of squamous epithelium

text: plasmid (ampicillin resistant)


characteristic: External Id

text: University of Bonn

text: live

text: Generic

text: cDNA library was contructed with the Clontech cDNA library constrution kit

text: morula stage embryo

text: Bos taurus

text: bovine single morula stage embryo cDNA library