characteristic: External Id

text: live

text: Generic

text: 129.3 ES cells

characteristic: cell-line

text: Mouse cDNA project by the Laboratory of Genetics, National Institute on Aging (NIA), Intramural Research Program, NIH (http://lgsun.grc.nia.nih.gov/cDNA). This is a long-transcript enriched cDNA library (Ref. Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]). ES cells were plated at density 3x104/cm2, on gelatin-coated plates and cultured for 48 hrs at 37 OC, 5% CO2. Culture medium: DMEM supplemented with 15% FBS, 2 mM L-glutamine, 0.1 mM NEAA, 1mM Sodium pyruvate, 0.1 mM beta-mercaptoethanol, 1000 U/ml LIF, 100 U/ml penicillin, and 100 ug/ml streptomycin. Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 2ug of total RNA, treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-S. The products were purified by phenol/chloroform and Centricon 100. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pCMV-SPORT6 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 2.7 kb. The library was constructed by Yulan Piao.

text: Mus musculus

text: SalI

text: NotI

text: Male

characteristic: sex

text: Embryonic Stem Cell

text: NIA Mouse Embryonic Stem (ES) cell (Lif+, 48 h, high density) cDNA library (Long)

text: plasmid (ampicillin resistant)

text: pCMV-SPORT6 (Invitrogen)


status: PUBLIC

text: National Institute on Aging/National Institutes of Health

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

characteristic: NCBI submission package

text: 129.3 ES cells

tag: attribute

text: Mouse cDNA project by the Laboratory of Genetics, National Institute on Aging (NIA), Intramural Research Program, NIH (http://lgsun.grc.nia.nih.gov/cDNA). This is a long-transcript enriched cDNA library (Ref. Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]). ES cells were plated at density 3x103/cm2, on gelatin-coated plates and cultured for 48 hrs at 37 OC, 5% CO2. Culture medium: DMEM supplemented with 15% FBS, 2 mM L-glutamine, 0.1 mM NEAA, 1mM Sodium pyruvate, 0.1 mM beta-mercaptoethanol, 100 U/ml penicillin, and 100 ug/ml streptomycin. Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 2ug of total RNA, treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-S. The products were purified by phenol/chloroform and Centricon 100. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pCMV-SPORT6 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 2.8 kb. The library was constructed by Yulan Piao.

characteristic: description

characteristic: lab-host

text: Mus musculus

characteristic: organism

text: SalI

text: NotI

text: Male

characteristic: strain

text: Embryonic Stem Cell

characteristic: tissue-type

text: NIA Mouse Embryonic Stem (ES) cell (Lif-, 48 h, low density) cDNA library (Long)

characteristic: title

text: plasmid (ampicillin resistant)

text: pCMV-SPORT6 (Invitrogen)

characteristic: vector


text: live

text: Generic

text: 129.3 ES cells

text: Mouse cDNA project by the Laboratory of Genetics, National Institute on Aging (NIA), Intramural Research Program, NIH (http://lgsun.grc.nia.nih.gov/cDNA). This is a long-transcript enriched cDNA library (Ref. Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]). ES cells were plated at density 3x104/cm2, on gelatin-coated plates and cultured for 48 hrs at 37 OC, 5% CO2. Culture medium: DMEM supplemented with 15% FBS, 2 mM L-glutamine, 0.1 mM NEAA, 1mM Sodium pyruvate, 0.1 mM beta-mercaptoethanol, 100 U/ml penicillin, and 100 ug/ml streptomycin. Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 2ug of total RNA, treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-S. The products were purified by phenol/chloroform and Centricon 100. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pCMV-SPORT6 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 3.4 kb. The library was constructed by Yulan Piao.

text: Mus musculus

text: SalI

text: NotI

text: Male

text: Embryonic Stem Cell

text: NIA Mouse Embryonic Stem (ES) cell (Lif-, 48 h, high density) cDNA library (Long)

text: plasmid (ampicillin resistant)

text: pCMV-SPORT6 (Invitrogen)


text: live

text: Generic

text: Mouse cDNA project by the Laboratory of Genetics, National Institute on Aging (NIA), Intramural Research Program, NIH (http://lgsun.grc.nia.nih.gov/cDNA). This is a long-transcript enriched cDNA library (Ref. Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]). Total RNAs were extracted from a pool of 8 embryos at 10.5-days postcoitum. Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 2ug of total RNA, treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-S. The products were purified by phenol/chloroform and Centricon 100. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pCMV-SPORT6 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 3.4Kb. The library was constructed by Yulan Piao.

characteristic: dev-stage

text: Mus musculus

text: SalI

text: NotI

text: whole embryo including extraembryonic tissues at 10.5-days postcoitum

text: NIA Mouse E10.5 whole embryo cDNA library (Long)

text: plasmid (ampicillin resistant)

text: pCMV-SPORT6 (Invitrogen)


characteristic: External Id

text: live

text: Generic

text: Mouse cDNA project by the Laboratory of Genetics, National Institute on Aging (NIA), Intramural Research Program, NIH (http://lgsun.grc.nia.nih.gov/cDNA). This is a long-transcript enriched cDNA library (Ref. Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]). Total RNAs were extracted from a pool of 3 embryos at 11.5-days postcoitum. Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 2ug of total RNA, treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-S. The products were purified by phenol/chloroform and Centricon 100. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pCMV-SPORT6 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 3.3Kb. The library was constructed by Yulan Piao.

text: Mus musculus

text: SalI

text: NotI

text: whole embryo including extraembryonic tissues at 11.5-days postcoitum

text: NIA Mouse E11.5 whole embryo cDNA library (Long)

text: plasmid (ampicillin resistant)

text: pCMV-SPORT6 (Invitrogen)


text: live

text: Generic

text: Mouse cDNA project by the Laboratory of Genetics, National Institute on Aging (NIA), Intramural Research Program, NIH (http://lgsun.grc.nia.nih.gov/cDNA). This is a long-transcript enriched cDNA library (Ref. Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]). The mRNAs were extracted from a pool of 360 embryos at 8-cell stage. Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 18ng of mRNA, treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-S. The products were purified by phenol/chloroform and Centricon 100. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pCMV-SPORT6 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 2.7Kb. The library was constructed by Yulan Piao.

text: Mus musculus

text: SalI

text: NotI

text: 8-cell stage embryo

text: NIA Mouse eight-cell-Embryo cDNA library (Long)

text: plasmid (ampicillin resistant)

text: pCMV-SPORT6 (Invitrogen)


text: live

text: Generic

text: Mouse cDNA project by the Laboratory of Genetics, National Institute on Aging (NIA), Intramural Research Program, NIH (http://lgsun.grc.nia.nih.gov/cDNA). This is a long-transcript enriched cDNA library (Ref. Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]). The mRNAs were extracted from a pool of 360 embryos at 4-cell stage. Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 10.8ng of mRNA, treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-S. The products were purified by phenol/chloroform and Centricon 100. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pCMV-SPORT6 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 2.2Kb. The library was constructed by Yulan Piao.

text: Mus musculus

text: SalI

text: NotI

text: 4-cell stage embryo

text: NIA Mouse four-cell-Embryo cDNA library (Long)

text: plasmid (ampicillin resistant)

text: pCMV-SPORT6 (Invitrogen)


text: Third Military Medical University of ChongQing, China

text: live

text: Generic

text: hepatocellular carcinoma cDNA Lib obtained by SSH(Suppression subtractive hybridization) EST obtained by SSH and identified by northern blot.

text: operative sample

text: Homo sapiens

text: EcoRV T-Cloning site

text: hepatocellular carcinoma tissues

text: homo sapiens (human) liver cancer cDNA Lib

text: T-A cloning vector(plasmid)

text: PMD18-T vector


text: Roslin Institute

text: live

text: Generic

text: Premade library from Stratagene (cat no. 935402)

text: 7 weeks

text: Liver

characteristic: organ

text: Gallus gallus

text: EcoRI

text: Male

text: Broiler breeder

text: Chicken 7 weeks liver cDNA library

text: Lambda ZAP


text: ARC Seibersdorf Research GesmbH

text: live

text: Generic

text: AdvanTAge PCR Cloning Kit; Clontech: PT3067-5

text: E.coli TOP10F'

text: Quercus petraea

text: callus cell culture

text: Oak subtraction cDNA library

text: T/A cloning vector

text: pT-Adv


text: National Research Council of Canada

text: live

text: Generic

text: Zygotic embryos at torpedo stage from Brassica napus seeds were used for the isolation of PolyA RNA and in the construction of the cDNA library. Sequences have been trimmed to remove vector and low quality regions using LUCY sequence cleanup software (www.tigr.org).

text: Brassica napus

text: Sfi IA

text: Sfi IB

text: Brassica napus 26RDBNT

text: plasmid


text: National Research Council of Canada

text: live

text: Generic

text: Zygotic mature embryos from Brassica napus seeds were used for the isolation of PolyA RNA and in the construction of the cDNA library. Sequences have been trimmed to remove vector and low quality regions using LUCY sequence cleanup software (www.tigr.org).

text: Brassica napus

text: Sfi IA

text: Sfi IB

text: Brassica napus 25RDBNM

text: plasmid


text: National Research Council of Canada

text: live

text: Generic

text: Sequences have been trimmed to remove vector and low quality regions using LUCY sequence cleanup software (www.tigr.org).

text: Brassica napus

text: Sfi IA

text: Sfi IB

text: Brassica napus 17ACDHMS

text: plasmid


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: SfiIA

text: SfiIB

text: Brassica napus 3ETMS

text: plasmid


text: National Research Council of Canada

text: live

text: Generic

text: Zygotic embryos at heart stage from Brassica napus seeds were used for the isolation of PolyA RNA and in the construction of the cDNA library. Sequences have been trimmed to remove vector and low quality regions using LUCY sequence cleanup software (www.tigr.org).

text: Brassica napus

text: Sfi I A

text: Sfi I B

text: Brassica napus 24RDBNH

text: plasmid


text: Plant Biotechnology Institute, National Research Council Canada

text: live

text: Generic

text: Brassica napus

text: Sfi I A

text: Sfi I B

text: Brassica seedling 10FGYSUA

text: plasmid


text: National Research Council of Canada

text: live

text: Generic

text: Brassica napus

text: Sfi IA

text: Sfi IB

text: Brassica napus 4ETGS72h

text: plasmid


text: live

text: Generic

text: Brassica napus

text: Brassica napus EX20LIB1

text: plasmid

text: pGemT-Easy


text: National Research Council of Canada

text: live

text: Generic

text: mRNA from 5-day old seeds of Brassica napus was used to construct a cDNA library in pDNR-LIB-CREATOR (Clontech). DNA sequencing was done by the NRC-PBI Sequencing Unit, and trimming of the vector and low quality sequences was performed by the NRC-PBI Bioinformatics Unit. Sequences have been trimmed to remove vector and low quality regions using LUCY sequence cleanup software (www.tigr.org).

text: Brassica napus

text: Brassica napus BNDH5DCT

text: plasmid


text: live

text: Generic

text: Brassica napus

text: Brassica napus EX20LIB3

text: plasmid

text: pGemT-Easy


text: live

text: Generic

text: Brassica napus

text: Sfi I A

text: Sfi I B

text: Brassica seedling 11FGYSDB

text: plasmid


text: live

text: Generic

text: Brassica napus

text: Brassica napus EX20LIB4

text: plasmid

text: pGemT-Easy


text: National University of Singapore

text: live

text: Generic

text: Library was prepared by Sew Yun Shin, Universiti Putra Malaysia, Malaysia and Kavita Reginald and Gao Yunfeng, National University of Singapore, Singapore.

text: Suidasia medanensis

text: Suidasia medanensis cDNA library


text: National University of Singapore

text: live

text: Generic

text: Library was prepared by Sheik Fadil Ryan Ramjan, National University of Singapore, Singapore.

text: Aleuroglyphus ovatus

text: Aleuroglyphus ovatus cDNA library


text: National University of Singapore

text: live

text: Generic

text: Library was prepared by Hema Jethanand, Sheik Fadil Ryan Ramjan and Lai Jesyin, National University of Singapore, Singapore.

text: Tyrophagus putrescentiae

text: Tyrophagus putrescentiae cDNA library


text: INRA Rennes

text: live

text: Generic

characteristic: cultivar

text: Sample name: ApAL3SD ; Plant growth place: INRA-Rennes, UMR BiO3P, BP 35327, 35653 Le Rheu cedex, France ; Soil conditions: peat ; Sowing date: 25/03/2003 ; Harvesting date: 10/04/2003 ; Stress date: no stress ; Description: aphids inoculated on one-week old Vicia faba germinations under non sterile conditions. ; experimental condition: short photoperiod (12-hr light/12-hr dark at 18 c)

text: third instar nymph (L3)

text: Acyrthosiphon pisum

text: SfiIA

text: SfiIB

text: antennae

text: pDNR-LIB


text: INRA Rennes

characteristic: INSDC center name

text: live

text: Generic

text: inra-lyon-bf2i ll01

text: Sample name: ApDT ; Plant growth place: UMR INRA-INSA BF2I ; Sowing date: 03/04/2003 ; Harvesting date: 12/06/2003 ; Description: Long photoperiod 16-hr light/8-hr dark at 20 C; no overcrowding; grown on Vicia faba 3-wk old seedlings sown under non sterile condition. ; experimental condition: standard, see description

text: adults, parthenogenetic females

text: Acyrthosiphon pisum

text: SfiIA

text: SfiIB

text: digestive track

text: ApDT

text: pDNR-LIB


text: INRA Rennes

characteristic: INSDC first public

text: live

text: Generic

text: Sample name: ApHL3LD ; Plant growth place: INRA-Rennes, UMR BiO3P, BP 35327, 35653 Le Rheu cedex, France ; Soil conditions: peat ; Sowing date: 18/01/2003 ; Harvesting date: 03/02/2003 ; Stress date: no stress ; Description: aphids inoculated on one-week old Vicia faba germinations under non sterile conditions. ; experimental condition: long photoperiod (16-hr light/8-hr dark at 18 c)

text: third instar nymph (L3)

text: Acyrthosiphon pisum

text: SfiIA

text: SfiIB

text: head

text: pDNR-LIB


text: INRA Rennes

characteristic: INSDC last update

text: live

text: Generic

text: Sample name: ApHL3SD ; Plant growth place: INRA-Rennes, UMR BiO3P, BP 35327, 35653 Le Rheu cedex, France ; Soil conditions: peat ; Sowing date: 20/03/2003 ; Harvesting date: 10/04/2003 ; Stress date: no stress ; Description: aphids inoculated on one-week old Vicia faba germinations under non sterile conditions. ; experimental condition: short photoperiod (12-hr light/12-hr dark at 18 c)

text: third instar nymph (L3)

text: Acyrthosiphon pisum

text: SfiIA

text: SfiIB

text: head

text: pDNR-LIB


text: INRA Rennes

text: live

characteristic: INSDC status

text: Generic

text: developmentstage

text: Sample name: ID0AAA ; Plant growth place: Department of Ecology & Evolutionary Biology, Princeton University ; Soil conditions: Soil ; Sowing date: 01/06/1999 ; Harvesting date: 01/06/1999 ; Stress date: no stress ; Description: Aphids inoculated on one-week old Vicia faba under non-sterile conditions. All parthenogenetic stages and both winged and wingless adults were collected for library construction. ; experimental condition: long photoperiod (16-hr light/8-hr dark at 18 c)

text: nymphs and adults (parthenogenetic females)

text: Acyrthosiphon pisum

text: EcoRI

text: XhoI

text: whole insect

text: ApMS

text: pBS-SK minus


text: University of Delaware

text: live

text: Generic

characteristic: NCBI submission model

text: E.coli EMDH10B

text: Gallus gallus

text: Male and Female

characteristic: sex

text: thymus, bursa, spleen, PBL, bone marrow

text: Chicken Lymphoid cDNA library (pgn2c)

text: pCMVSPORT 6


text: University of Minnesota

text: live

text: Generic

characteristic: NCBI submission package

text: Fibroblast

characteristic: cell type

text: Library constructed with Avian Pneumovirus subtype C (United States Colorado Strain) infected chicken embryo fibroblast cells

characteristic: description

text: 10-day old embryo

text: Gallus gallus

text: Suppression subtractive hybridization library


text: AgResearch

text: live

text: Generic

text: Germ cell

text: Suppresive subtraction hybridisation library as per Clontech, cloned into pGem

text: GV-stage oocyte

text: Bos taurus

characteristic: organism

text: Ovary

text: Bovine oocyte SSH-pGEM Library

text: pGem


text: Auburn University

text: live

text: Generic

text: One year old P. trifoliata plants were grown for 5 weeks in a growth chamber with a 12 h light period at 400 mol m-2s-1 intensity. The temperature decline regime was as follows: 32oC day/21oC night (14 days); 27oC day/16oC night (7days); 24oC day/13oC night (7days) and 18oC day/7oC night (7days). RNA from the end of second week was used as an unacclimated control and RNA from the end of fifth week as treatment. mRNA Differential display was performed using six RNAimage kits and a total of 144 primer pairs according to the protocol enclosed (GenHunter, Nashville, TN, U.S.A.)

text: GH Competent Cells

characteristic: lab-host

text: Citrus trifoliata

text: Poncirus trifoliata cold acclimation with DDRT-PCR

text: Insert-ready PCR-TRAP Cloning Vector


text: Tianjin Endocrinology Institute

text: live

text: Generic

text: cDNA fragment related to the brain of hyperthyroid hormonal rat

text: Rattus norvegicus

text: brain

characteristic: tissue-type

text: Wistar rat brain

characteristic: title


text: Brigham Young University

text: live

text: Generic

text: Real

text: Floral cDNA Library from Chenopodium quinoa

text: Pre-anthesis

characteristic: dev-stage

text: XL-1 Blue

text: Flowers

text: Chenopodium quinoa

text: SfiI

text: SfiI

text: cqfloral

characteristic: vector


characteristic: External Id

text: Brigham Young University

text: live

text: Generic

text: Real

text: Developing Seed cDNA Library from Chenopodium quinoa

text: XL-1 Blue

text: Chenopodium quinoa

text: SfiI

text: SfiI

text: Developing Seed

text: cqseed


text: Unite IPMSV, Inra

text: live

text: Generic

text: second stage juvenile

text: Meloidogyne incognita

text: Meloidogyne incognita second stage juvenile


text: live

text: Generic

text: Aplysia californica

text: central nervous system

text: Aplysia californica central nervous system


text: RIKEN Brain Science Institute

text: live

text: Generic

text: Mus musculus

text: mixed

text: cerebellum

text: Mus musculus cerebellum E18-P56


text: RIKEN Brain Science Institute

text: live

text: Generic

text: Mus musculus

text: mixed

text: cerebellum

text: Mus musculus cerebellum E18-P57


text: RIKEN Brain Science Institute

text: live

text: Generic

text: Mus musculus

text: mixed

text: cerebellum

text: Mus musculus cerebellum E18-P58


text: RIKEN Brain Science Institute

text: live

text: Generic

text: Mus musculus

text: mixed

text: cerebellum

text: Mus musculus cerebellum E18-P59


text: RIKEN Brain Science Institute

text: live

text: Generic

text: Mus musculus

text: mixed

text: cerebellum

text: Mus musculus cerebellum E18-P60


text: RIKEN Brain Science Institute

text: live

text: Generic

text: Mus musculus

text: mixed

text: cerebellum

text: Mus musculus cerebellum E18-P61


text: RIKEN Brain Science Institute

text: live

text: Generic

text: Mus musculus

text: mixed

text: cerebellum

text: Mus musculus cerebellum E18-P62


text: RIKEN Brain Science Institute

text: live

text: Generic

text: Mus musculus

text: mixed

text: cerebellum

text: Mus musculus cerebellum E18-P63


text: RIKEN Brain Science Institute

text: live

text: Generic

text: Mus musculus

text: mixed

text: cerebellum

text: Mus musculus cerebellum E18-P64


text: RIKEN Brain Science Institute

text: live

text: Generic

text: Mus musculus

text: mixed

text: cerebellum

text: Mus musculus cerebellum E18-P65


text: RIKEN Brain Science Institute

text: live

text: Generic

text: Mus musculus

text: mixed

text: cerebellum

text: Mus musculus cerebellum E18-P66


text: RIKEN Brain Science Institute

text: live

text: Generic

text: Mus musculus

text: mixed

text: cerebellum

text: Mus musculus cerebellum E18-P67


text: RIKEN Brain Science Institute

text: live

text: Generic

text: Mus musculus

text: mixed

text: cerebellum

text: Mus musculus cerebellum E18-P68


text: RIKEN Brain Science Institute

text: live

text: Generic

text: Mus musculus

text: mixed

text: cerebellum

text: Mus musculus cerebellum E18-P69


text: RIKEN Brain Science Institute

text: live

text: Generic

text: Mus musculus

text: mixed

text: cerebellum

text: Mus musculus cerebellum E18-P70


text: RIKEN Brain Science Institute

text: live

text: Generic

text: Mus musculus

text: mixed

text: cerebellum

text: Mus musculus cerebellum E18-P71


text: RIKEN Brain Science Institute

text: live

text: Generic

text: Mus musculus

text: mixed

text: cerebellum

text: Mus musculus cerebellum E18-P72


text: RIKEN Brain Science Institute

text: live

text: Generic

text: Mus musculus

text: mixed

text: cerebellum

text: Mus musculus cerebellum E18-P73


text: Jimei University

text: live

text: Generic

text: putative immune-related sequences

text: Haliotis diversicolor supertexta

text: Haliotis diversicolor supertexta library


text: Microbial Ecology, Institution of Ecology, Lund University

text: live

text: Generic

text: This EST clone is originating from one of four cDNA libraries. These libraries were constructed for transcript profiling of nematophagous ascomycete Monacrosporium haptotylum in interaction with Caenorhabditis elegans. The library 'MYCELIUM' is constructed from vegetative mycelium in a saprophytic stage, the second library 'KNOB' is constructed from the separated infection structure and finally the '4h-POST INFECTION' and the '24h-POST INFECTION' libraries represent early (4h) and late (24h) post-infection stages. These libraries where analayzed in parallel and 1729 (MYCELIUM), 1450 (KNOB), 1195 (4h-POST INFECTION) and 2741 (24h-POST INFECTION) high-quality (PHRED 20) ESTs of >99bp have been deposited. The cDNA libraries were constructed from total RNA using the SMART cDNA library Construction kit (#K1051-1, Clontech, Palo Alto, CA, USA) according to the manufacturer's instructions. Full-length cDNAs were trimmed by SfiI, fractionated and directionally ligated into (lambda)pTriplEx2 arms. The lambda library was converted to a plasmid library via site-specific recombination at loxP sites in a Cre+ strain (E. coli XL1-Blue). Plasmid clones were randomly collected and analysed by DNA sequencing using a plasmid-specific forward primer (P104).

text: Saprophytic

text: Escherichia coli XL1-Blue

text: Dactylellina haptotyla

text: SfiI

text: SfiI

text: CBS 200.50

characteristic: strain

text: Vegetative mycelium

text: MYCELIUM

text: Plasmid


characteristic: INSDC center name

text: live

text: Generic

text: Infection Structure

text: Escherichia coli XL1-Blue

text: Dactylellina haptotyla

text: SfiI

text: SfiI

text: CBS 200.50

text: Infection structure

text: KNOB

text: Plasmid


text: Mahidol University

characteristic: INSDC first public

text: live

text: Generic

text: The cDNA library was generated as part of Penaeus monodon EST project. Total RNA was isolated from lymphoid organ of the YHV infected Penaeus monodon. The mRNA was further purified, reverse transcribed and cloned to Uni-ZAP XR.

text: Penaeus monodon

text: EcoR I

text: Xho I

text: Lymphoid organ

text: Lymphoid organ - YHV infected library

text: Phage

text: Uni-ZAP XR


text: Kyoto University

characteristic: INSDC last update

text: live

text: Generic

text: adult

text: Ciona intestinalis

text: digestive gland

text: Yutaka Satou unpublished cDNA library, adult digestive gland


text: Kyoto University

text: live

characteristic: INSDC status

text: Generic

text: embryo

text: Ciona intestinalis

text: whole animal

text: Yutaka Satou unpublished cDNA library, embryo whole animal


text: University of Maryland

text: live

text: Generic

characteristic: NCBI submission model

text: Metarhizium anisopliae was grown in insect haemolymph for 24 hours. A cDNA library was constructed in the vector pCMV.SPORT6.1

text: Metarhizium anisopliae

text: Metarhizium anisopliae ARSEF 2575 from insect blood


text: University of Maryland

text: live

text: Generic

text: Metarhizium anisopliae was grown in bean root extract for 24 hours. A cDNA library was constructed in the vector pCMV.SPORT6.1

text: Metarhizium anisopliae

text: Metarhizium anisopliae ARSEF 2575 from bean root extract


text: Texas A&M University

text: live

text: Generic

text: The subtracted PCR products were cloned into Pinpoint Xa-1 T-vector after suppression subtractive hybridization with dealate-mating queen as 'tester' and alate-virgin queen as 'driver'

text: queen

text: Solenopsis invicta

text: female

characteristic: sex

text: Suppression subtractive hybridization (SSH) library of Solenopsis invicta

text: Pinpoint Xa-1 T-vector


text: Universities of Nottingham and Edinburgh

text: live

text: Generic

characteristic: NCBI submission package

text: lambda ZAP II cDNA library constructed in Japan by Hisayo Sadamoto, Laboratory of Animal Behavior and Intelligence, Division of Biological Sciences, Graduate School of Science, Hokkaido University, North 10, West 8, Kita-ku, Sapporo 060-0810, Japan email sada@sci.hokudai.ac.jp

text: Lymnaea stagnalis

text: Lymnaea stagnalis J1

text: lambda ZAP II


text: live

text: Generic

text: The library was constructed in the Netherlands by Dr Ronald van Kesteren, Dept. Molecular & Cellular Neurobiology, Research Institute Neurosciences, Vrije Universiteit, De Boelelaan 1087, 1081 HV Amsterdam, The Netherlands, email revankes@bio.vu.nl

text: Lymnaea stagnalis

text: central nervous system

text: Lymnaea stagnalis N1


text: USDA-ARS and University of Minnesota

text: live

text: Generic

text: Ogle-C (a reselection from cv. Ogle)

characteristic: cultivar

text: Seed were germinated in a dark incubator at 20 C. At 6 days roots were harvested, total RNA and poly(A) were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the HW Rines and CP Vance USDA-ARS labs (Rines, Vance, Miller, Taller) at the University of Minnesota, St. Paul, MN. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson USDA-ARS lab (Anderson, Crossman, Lazo) in Albany, CA.

text: E. coli SOLR (excised) E. coli TJC121 (sequencing)

text: Avena sativa

text: EcoRI

text: XhoI

text: root

text: Lib AA071E1X Avena sativa cv. Ogle-C root

text: Lambda Uni-ZAP XR, excised phagemid


text: USDA-ARS and University of Minnesota

text: live

text: Generic

text: Ogle-C (a reselection from cv. Ogle)

text: Plants were grown in a growth chamber at 20 C day, 15 C night in 12 hour day light period. At 3 weeks leaves were harvested, total RNA and poly(A) were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the HW Rines and CP Vance USDA-ARS labs (Rines, Vance, Miller, Taller) at the University of Minnesota, St. Paul, MN. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson USDA-ARS lab (Anderson, Crossman, Lazo) in Albany, CA.

characteristic: description

text: Avena sativa

text: EcoRI

text: XhoI

text: green leaf

text: Lib AA070E1X Avena sativa cv. Ogle-C green leaf

text: Lambda Uni-ZAP XR, excised phagemid


text: USDA-ARS and University of Minnesota

text: live

text: Generic

text: Ogle-C (a reselection from cv. Ogle)

text: Seed were germinated in a dark incubator at 20 C. At 6 days etiolated leaves were harvested, total RNA and poly(A) were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the HW Rines and CP Vance USDA-ARS labs (Rines, Vance, Miller, Taller) at the University of Minnesota, St. Paul, MN. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson USDA-ARS lab (Anderson, Crossman, Lazo) in Albany, CA.

text: Avena sativa

characteristic: organism

text: EcoRI

text: XhoI

text: etiolated leaf

text: Lib AA069E1X Avena sativa cv. Ogle-C etiolated leaf

text: Lambda Uni-ZAP XR, excised phagemid


text: Beijing Institute of Microbiology and Epidemiology

text: live

text: Generic

text: Total RNA was extracted from asynchronous blood stage forms of RQ and K173 strain of P. berghei, grown in mouse to 20-30% parasitemia. cDNA was synthesized by super SMART PCR cDNA Synthesis Kit(Clontech). Taking RQ as tester and K173 as driver, SSH PCR was performed by PCR-Select cDNA Subtraction Kit (Clontech). The SSH cDNA cloned into pMD 18-T Vector.

text: erythrocytic stage

text: E. coli JM109

characteristic: lab-host

text: Plasmodium berghei

text: RQ strain (the artemisinin-resistant strain develped from K173)

text: SSH library of Plasmodium berghei strain RQ

text: Plasmid

text: pMD 18-T


text: live

text: Generic

text: Total RNA was extracted from asynchronous blood stage forms of K173 and RQ(artemisinin-resistant strain developed from K173) strains of P. berghei, grown in mouse to 20-30% parasitemia. cDNA was synthesized by super SMART PCR cDNA Synthesis Kit(Clontech).Taking K173 as tester and RQ as driver, SSH PCR was performed by PCR-Select cDNA Subtraction Kit (Clontech). The SSH cDNA cloned into pMD 18-T Vector.

text: erythrocytic stage

text: E. coli JM109

text: Plasmodium berghei

text: k173 strain

text: SSH library of Plasmodium berghei strain K173

text: Plasmid

text: pMD 18-T


text: National Cancer Institute / NIH

text: live

text: Generic

text: 1st strand cDNA was primed with an oligo(dT) primer [GCGGCTGAAGACGGCCTATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded cDNA was ligated to a DraIII adaptor [GGCCUACUGG], digested and directionally cloned into distinct DraIII sites of the pME18S-FL3. Library was size selected for 1.0 kb, with a average insert size of ~1.2kb. Library constructed by Yutaka Suzuki (University of Tokyo Institute of Medical Science). Custom primers recommended for sequencing: 5' end primer 5'-GGATGTTGCCTTTACTTCTA-3' and 3' end primer 5'-CGACCTGCAGCTCGAGCACA-3'. Note: This is a Zebrafish Gene Collection (ZGC) library

text: DH10B TonA

text: embryo

characteristic: organ

text: Danio rerio

text: DraIII

text: DraIII

text: Zebrafish Danio Rerio AB wild type 18-20 hpf

characteristic: tissue-type

text: phagemid


text: National Cancer Institute / NIH

text: live

text: Generic

text: DH10B TonA

text: Danio rerio

text: DraIII

text: DraIII

text: mixed

characteristic: title

text: phagemid


text: University of Edinburgh

text: live

text: Generic

text: Ovis aries

text: Sheep spleen\brain pSport1 library

characteristic: vector


characteristic: External Id

text: Jawaharlal Nehru University

text: live

text: Generic

text: Xanthii

text: E.coli

text: Nicotiana tabacum

text: Pollen

text: Nicotiana tabacum Pollen PCR-based subtractive library

text: Plasmid

text: p-GEMT Easy


text: Eugentech / KRIBB

text: live

text: Generic

text: cDNAs from MeJA treated ginseng hairy root were cloned into lambda ZAP XR vector

text: Panax ginseng

text: MeJA treated hairy root

text: Ginseng cDNA library from MeJA treated hairy root

text: lambda ZAP XR


text: The Horticulture and Food Research Institute of New Zealand Ltd

text: live

text: Generic

text: Library sequenced by Genesis Research & Development

text: 59 days after full bloom, seeds removed

characteristic: dev-stage

text: Malus domestica

text: fruit

text: (AAAA) Royal Gala 59 DAFB fruit, seeds removed

text: pBK-CMV


characteristic: INSDC center name

text: live

text: Generic

text: Pre-opening

text: Malus domestica

text: Vegetative bud

text: (AACA) Royal Gala pre-opening vegetative bud, normalised 72h

text: pBK-CMV


characteristic: INSDC first public

text: live

text: Generic

text: Pre-opening

text: Malus domestica

text: Vegetative bud

text: (AADA) Royal Gala pre-opening vegetative bud, normalised 24h

text: pBK-CMV


characteristic: INSDC last update

text: live

text: Generic

text: Pre-opening

text: Malus domestica

text: Vegetative bud

text: (AAEA) Royal Gala pre-opening vegetative bud, normalised 5h

text: pBK-CMV


text: live

characteristic: INSDC status

text: Generic

text: 150 days after full bloom

text: Malus domestica

text: Fruit skin peel

text: (AAFB) Royal Gala apple skin peel, tree-ripened fruit 150 DAFB

text: pBK-CMV


text: live

text: Generic

characteristic: NCBI submission model

text: Expanding

text: Malus domestica

text: Leaf

text: (AAGA) Pinkie expanding leaf, normalised 0h +M13

text: pBK-CMV


text: live

text: Generic

text: Expanding

text: Malus domestica

text: Leaf

text: (AAHA) Pinkie expanding leaf, normalised 5h +M13

text: pBK-CMV


text: live

text: Generic

text: Expanding

text: Malus domestica

text: Leaf

text: (AAIA) Pinkie expanding leaf, normalised 24h +M13

text: pBK-CMV


text: live

text: Generic

text: Expanding

text: Malus domestica

text: Leaf

text: (AAJA) Pinkie expanding leaf, normalised 24h -M13

text: pBK-CMV


text: live

text: Generic

text: Expanding

text: Malus domestica

text: Leaf

text: (AAKA) Pinkie expanding leaf, normalised 72h +M13

text: pBK-CMV


text: live

text: Generic

text: 150 days after full bloom

text: Malus domestica

text: Fruit cortex

text: (AALA) Royal Gala 150 DAFB fruit cortex

text: pBluescript SK(-)


text: live

text: Generic

text: 150 days after full bloom

text: Malus domestica

text: Fruit cortex

text: (AALB) Royal Gala 150 DAFB fruit cortex

text: pBK-CMV


text: live

text: Generic

text: Early, flower differentiation just starting

text: Malus domestica

text: Floral bud

text: (AAMA) Royal Gala spur bud autumn

text: pBluescript SK(-)


text: live

text: Generic

text: Partially senescing leaves

text: Malus domestica

text: Leaf

text: (AANA) Royal Gala partially senescing leaf

text: pBluescript SK(-)


text: live

text: Generic

text: Malus domestica

text: Phloem, scrapings from inside of bark mature wood

text: (AAOA) Royal Gala phloem

text: pBluescript SK(-)


text: live

text: Generic

text: 24 days after full bloom

text: Malus domestica

text: Fruit

text: (AAPA) Royal Gala 24 DAFB fruit

text: pBK-CMV


text: live

text: Generic

text: Partially senescing leaf

text: Malus domestica

text: Leaf

text: (AARA) Royal Gala partially senescing leaf

text: pBK-CMV


text: live

text: Generic

text: 10 days after full bloom

text: Malus domestica

text: Fruit

text: (AASA) Royal Gala 10 DAFB fruit

text: pBluescript SK(-)


text: live

text: Generic

text: 10 days after full bloom

text: Malus domestica

text: Young fruit

text: (AASB) Royal Gala 10 DAFB fruit

text: pBluescript SK(-)


text: live

text: Generic

text: 10 days after full bloom

text: Malus domestica

text: Young fruit

text: (AASC) Royal Gala 10 DAFB fruit

text: pBluescript SK(-)


text: live

text: Generic

text: 87 days after full bloom

text: Malus domestica

text: Fruit cortex

text: (AAUA) Royal Gala 87 DAFB fruit cortex

text: pBluescript SK(-)


text: live

text: Generic

text: Mature

text: Malus domestica

text: Fruit cortex

text: (AAVB) Royal Gala cool-stored, warmed and recooled fruit cortex

text: pBluescript SK(-)


text: The Horticulture and Food Research Institute of New Zealand Ltd

text: live

text: Generic

text: 59 days after full bloom

text: Malus domestica

text: Seed

text: (AAWA) Royal Gala 59 DAFB seeds

text: pBluescript SK(-)


text: live

text: Generic

text: Library sequenced by Genesis Research & Development

text: 126 days after full bloom

text: Malus domestica

text: Fruit core

text: (AAXA) Royal Gala 126 DAFB fruit core

text: pBluescript SK(-)


text: live

text: Generic

text: 126 days after full bloom

text: Malus domestica

text: Fruit cortex

text: (AAYA) Royal Gala 126 DAFB fruit cortex

text: pBluescript SK(-)


text: live

text: Generic

text: Mature wood

text: Malus domestica

text: Xylem, scrapings from stem after bark removed

text: (AAZA) M9 xylem

text: pBluescript SK(-)


text: live

text: Generic

text: Malus domestica

text: Spur buds from 'ON' trees

text: (ABAA) Pacific Rose spur buds from ON trees

text: pBluescript SK(-)


text: University of Florida

text: live

text: Generic

text: Differential display. cDNA synthesized by RT-PCR from RNA expressed in stems of fusiform rust susceptible Pinus elliottii seedlings 8 weeks after challenge with C. quercuum. Comparisons were made between pathogen- and water-challenged tissues.

text: Pinus elliottii/Cronartium quercuum f.sp. fusiforme mixed EST library

text: Fusiform rust susceptible slash pine challenged with C. quercuum f. sp. fusiforme


text: University of Florida

text: live

text: Generic

text: Differential display. cDNA was synthesized by RT-PCR from RNA expressed in above ground tissues isolated from Pinus elliottii seedlings treated to run-off with 5mM SA six times over three days. Comparisons were made between treated and nontreated materials.

text: Pinus elliottii

text: Salicylate-treated slash pine seedlings


text: University of Florida

text: live

text: Generic

text: Differential display. cDNA was synthesized by RT-PCR from RNA expressed in Pinus elliottii cell cultures after elicitation with chitosan (final concentration 60 ug/mL) for 24 hours. Comparisons were made between elicited and nonelicited cells.

text: Pinus elliottii

text: Chitosan treated slash pine cell cultures


text: University of Florida

text: live

text: Generic

text: Differential display. cDNA was synthesized by RT-PCR from RNA expressed in stems of pitch canker resistant Pinus elliottii seedlings 9 days after challenge with F. circinatum. Comparisons were made between pathogen- and water-challenged tissues.

text: Pinus elliottii

text: Pitch canker resistant slash pine challenged with F. circinatum


text: University of Florida

text: live

text: Generic

text: Differential display. cDNA was synthesized by RT-PCR from RNA expressed in stems of pitch canker susceptible Pinus elliottii seedlings 9 days after challenge with F. circinatum. Comparisons were made between pathogen- and water-challenged tissues.

text: Pinus elliottii/Fusarium circinatum mixed EST library

text: Pitch canker susceptible slash pine challenged with F. circinatum


text: University of Florida

text: live

text: Generic

text: Differential display. cDNA synthesized by RT-PCR from RNA expressed in stems of Pinus elliottii seedlings 24 hours after mechanical wounding with pliers. Comparisons were made between wounded and nonwounded tissues.

text: Pinus elliottii

text: Mechanically wounded slash pine seedlings


text: University of Georgia

text: live

text: Generic

text: cDNA from galled pine stems (infected with Cronartium quercuum f. sp. fusiforme) was hybridized to cDNA from healthy pine stems in order to enrich for sequences expressed in galled tissue, but not in healthy tissue. Sequences from this library may be derived from either the host (pine) or the pathogen (C. q. fusiforme).

text: stem

text: Pinus taeda/Cronartium quercuum f.sp. fusiforme mixed EST library

text: Galled pine stem suppression subtraction hybridization library


text: University of Georgia

text: live

text: Generic

text: cDNA from healthy pine stems was hybridized to cDNA from galled pine stems, which were infected with Cronartium quercuum f. sp. fusiforme, in order to enrich for sequences expressed in healthy stems, but not in galls.

text: stem

text: Pinus taeda

text: Healthy pine stem suppression subtraction hybridization library


text: Institute of Genetics and Developmental Biology, Chinese Academic Sciences

text: live

text: Generic

text: Triticum aestivum

text: Triticum aestivum cDNA-AFLP fragment


text: live

text: Generic

text: Puccinia striiformis

text: Puccinia striiformis cDNA-AFLP fragment


text: Kyoto University

text: live

text: Generic

text: juvenile

text: Ciona intestinalis

text: whole animal

text: Nori Satoh unpublished cDNA library, juvenile whole animal


text: Kyoto University

text: live

text: Generic

text: mature adult

text: Ciona intestinalis

text: whole animal

text: Nori Satoh unpublished cDNA library, mature adult whole animal


text: Kyoto University

text: live

text: Generic

text: stage3 juvenile

text: Ciona intestinalis

text: whole animal

text: Ciona intestinalis whole animal stage3 juvenile


text: The Horticulture and Food Research Institute of New Zealand Ltd

text: live

text: Generic

text: Library sequenced by Genesis Research & Development

text: Malus domestica

text: Spur buds from 'ON' trees

text: (ABAB) Pacific Rose spur buds from ON trees

text: pBluescript SK(-)


text: live

text: Generic

text: Malus domestica

text: Spur buds from 'OFF' trees

text: (ABBA) Pacific Rose spur buds from OFF trees

text: pBluescript SK(-)


text: live

text: Generic

text: Malus domestica

text: Spur buds from 'OFF' trees

text: (ABBB) Pacific Rose spur buds from OFF trees

text: pBluescript SK(-)


text: live

text: Generic

text: Mature

text: Malus domestica

text: Fruit cortex and skin

text: (ABCA) Royal Gala fruit stored at 0.5 degrees C for 24 hours

text: pBluescript SK(-)


text: live

text: Generic

text: Mature

text: Malus domestica

text: Fruit cortex and skin

text: (ABDA) Royal Gala fruit stored for 24 hours under low oxygen/high CO2

text: pBluescript SK(-)


text: live

text: Generic

text: Malus domestica

text: Leaf tissue

text: (ABKA) Royal Gala temperature stressed leaves

text: pBluescript SK(-)


text: live

text: Generic

text: Exponential phase of growth (three days after subculture)

text: Malus domestica

text: Cell culture

text: (ABLA) Braeburn cell culture three days after subculture


text: live

text: Generic

text: Malus domestica

text: Cell cultures

text: (ABLB) Braeburn cell culture three days after subculture


text: live

text: Generic

text: Malus domestica

text: Cell cultures

text: (ABLC) Braeburn cell culture three days after subculture

text: pBluescript SK(-)


text: live

text: Generic

text: Malus domestica

text: Shavings of phloem tissue

text: (ABMA) M9 phloem

text: pBluescript SK(-)


text: live

text: Generic

text: Malus domestica

text: Cultured fruit cells

text: (ABNA) Braeburn cultured fruit cells, boron exposed, phytoremediation


text: live

text: Generic

text: Malus domestica

text: Fruit cells

text: (ABNB) Braeburn cultured fruit cells, boron exposed, phytoremediation

text: pBluescript SK(-)


text: live

text: Generic

text: Mature

text: Malus sieboldii

text: Fruit

text: (ABOA) Crab apple mature fruit


text: live

text: Generic

text: Mature

text: Malus sieboldii

text: Fruit

text: (ABOB) Crab apple mature fruit

text: pBluescript SK(-)


text: live

text: Generic

text: Mature

text: Malus sieboldii

text: Fruit

text: (ABOC) Crab apple mature fruit

text: pBluescript SK(-)


text: live

text: Generic

text: Malus domestica

text: Root tips (distal 1.5 cm)

text: (ABPA) M9 root tips


text: live

text: Generic

text: Malus domestica

text: Root tips (distal 1.5 cm)

text: (ABPB) M9 root tips

text: pBluescript SK(-)


text: live

text: Generic

text: 0 days after full bloom

text: Malus domestica

text: Whole flowers

text: (ABQA) Royal Gala flower

text: pBluescript SK(-)


text: live

text: Generic

text: Expanding

text: Malus domestica

text: Leaf

text: (AEAA) Aotea expanding leaf

text: pBK-CMV


text: live

text: Generic

text: Young, expanding

text: Malus domestica

text: Leaf

text: (AELA) Royal Gala young expanding leaf

text: pBK-CMV


text: live

text: Generic

text: Expanding

text: Malus domestica

text: Leaf

text: (AENA) Northern Spy expanding leaf

text: pBK-CMV


text: live

text: Generic

text: Expanding

text: Malus domestica

text: Leaf

text: (AEPA) Pinkie expanding leaf

text: pBK-CMV


text: live

text: Generic

text: Pre-opening

text: Malus domestica

text: Floral bud

text: (AFBA) Royal Gala pre-opened floral bud

text: pBK-CMV


text: live

text: Generic

text: Pre-opening

text: Malus domestica

text: Floral bud

text: (AFBB) Royal Gala pre-opened floral bud

text: pBK-CMV


text: live

text: Generic

text: Pre-opening, flower at stage of nectar/pollen formation

text: Malus domestica

text: Floral bud

text: (AFBC) Royal Gala pre-opened floral bud

text: pBK-CMV


text: live

text: Generic

text: 24 days after full bloom

text: Malus domestica

text: Young fruit

text: (AOFA) Royal Gala 24 DAFB fruit

text: pBK-CMV


text: live

text: Generic

text: 59 days after full bloom

text: Malus domestica

text: Seed

text: (ASYA) Royal Gala 59 DAFB seeds

text: pBK-CMV


text: live

text: Generic

text: Pre-opening

text: Malus domestica

text: Vegetative bud

text: (AVBB) Royal Gala young shoot

text: pBK-CMV


text: live

text: Generic

text: Library sequenced by 1. Genesis Research & Development 2. Paradigm Genetics

text: Pre-opening

text: Malus domestica

text: Vegetative bud

text: (AVBC) Royal Gala young shoot

text: pBluescript SK(-)


text: live

text: Generic

text: 10 days after full bloom

text: Malus domestica

text: Young fruit

text: (AYFA) Royal Gala 10 DAFB fruit

text: pBK-CMV


text: live

text: Generic

text: 10 days after full bloom

text: Malus domestica

text: Young fruit

text: (AYFB) Royal Gala 10 DAFB fruit

text: pBK-CMV


text: live

text: Generic

text: testicle

text: Homo sapiens

text: Human testicle cDNA sequencing


text: Rosenstiel School of Marine and Atmospheric Science - University of Miami

text: live

text: Generic

text: Heart

text: Fundulus heteroclitus

text: Heart

text: Fundulus Heteroclitus Heart


text: live

text: Generic

text: Liver

text: Fundulus heteroclitus

text: Liver

text: Fundulus Heteroclitus Liver


text: Washington University School of Medicine

text: live

text: Generic

text: GoldRush

text: Total RNA was extracted from freeze dried fruit tissue Stage I- Young Fruitlet < 1 cm, using the method described by Wang and Vodkin (Plant Molecular Biology Reporter 12:132-145, 1994). Poly(A)+mRNA was isolated from total RNA using the PolyATtract mRNA Isolation system III (Promega). The library was prepared using the Invitrogen Life Technologies, Superscript Plasmid System with gateway technology for cDNA Synthesis and cloning. Complementary DNA was synthesized from mRNA using a Poly (dT) sequence with a NotI restriction site. Sal I linker adapters were ligated to the blunt ended cDNA fragments followed by restriction with NotI. The cDNA fragments were directionally cloned into the NotI-SalI restriction site of the pSPORT 1 vector. The ligated cDNA fragments were transformed into E. coli ElectroMax Dh10B host cells. Transformation efficiency: 10E+06 colony forming units Average insert size by PCR: 992 bp

text: DH10B ampicillin resistant

text: Malus domestica

text: NotI

text: SalI

text: Fruit

text: Mdfr

text: Phagemid (ampicillin resistant)


text: McGill University Health Centre Research Institute

text: live

text: Generic

text: DDRT-PCR was performed following the method decribed by Liang and Pardee (1992) using the RNAimage Kit (GenHunter Corporation) by Patricia Tellis in Dr Mario Chevrette laboratory.

text: Intact fin,1 day and 4 days regeneration

text: E. coli JM109

text: Caudal fin

text: Danio rerio

text: Mixed

characteristic: sex

text: Wild-type

characteristic: strain

text: DDRT-PCR amplified cDNA from regenerating caudal fin in Danio rerio

text: Plasmid

text: pGEM-T


text: Shandong Agricultural University; Chinese Academy of Agricultural Science

text: live

text: Generic

characteristic: NCBI submission package

text: Non-normalized library, sequenced 3' with TriplEx2 primer(TAATACGACTCACTATAGGG). Library constructed with total RNA extracted using the Trizol method and pooled from 3 females.

text: pre-oestrum

text: ovary

text: Ovis aries

text: Sfi I

text: Sfi I

text: female

text: Little-tailed Han Sheep

text: pre-oestrum adult sheep ovary library


text: The Institute for Genomic Research

text: live

text: Generic

text: Coccidioides posadasii spherule cDNA library, 0.5 to 5.3 kb

characteristic: description

text: spherules

text: E. coli DH10B, T1 phage resistant

text: Coccidioides posadasii

text: Not I

text: Eco RV

text: Plasmid

text: pExpress 1


text: The Institute for Genomic Research

text: live

text: Generic

text: Coccidioides posadasii spherule cDNA library, 0.4 to 2.3 kb

text: spherules

text: E. coli DH10B, T1 phage resistant

text: Coccidioides posadasii

characteristic: organism

text: Not I

text: Eco RV

text: Plasmid

text: pExpress 1


text: United States Dept of Agriculture

text: live

text: Generic

text: Williams 82

characteristic: cultivar

text: Subtraction library of soybean roots inoculated with Heterodera glycines (8, 12 and 20 days post-inoculation) subtracted with uninoculated root RNA and H. glycines egg RNA.

text: Glycine max

text: Root

text: Glycine max mixed library H. glycines, late library


text: United States Dept of Agriculture

text: live

text: Generic

text: Williams 82

text: Subtraction library of soybean roots inoculated with Heterodera glycines (1, 2 and 4 days post-inoculation) subtracted with uninoculated root RNA and H. glycines egg RNA.

text: Glycine max

text: Root

text: Glycine max mixed library H. glycines, early library


text: United States Dept of Agriculture

text: live

text: Generic

text: Heterodera glycines

text: Root

text: H. glycines mixed library Glycine max, late library


text: Chinese Academy of Sciences

text: live

text: Generic

text: Subtracted cDNA library prepared from headkidney and spleen of mandarin fish

text: 1 year

text: E.coli DH 5

characteristic: lab-host

text: Siniperca chuatsi

text: Male & female

text: headkidney and spleen

text: mandarin fish headkidney and spleen induced by Poly I: C

text: PGEM-T


text: Texas A&M University

text: live

text: Generic

text: The subtractive cDNA library, consisting of both forward and reverse subtractions, was prepared from polyA+ RNA from southern corn rootworm raised on diet with or without soyacystatin N, respectively. The clontech PCR-select cDNA subtraction kit protocol was followed in library construction.

text: 2nd instar

text: E. coli

text: Diabrotica undecimpunctata howardi

text: Subtractive cDNA library from southern corn rootworm larvae challenged by soyacystatin N

text: Plasmid

text: pCRII-TOPO


text: IRD (Institut de Recherche pour le Developpement)

text: live

text: Generic

text: Roots

characteristic: organ

text: Casuarina glauca

text: EcoRI

text: XhoI

text: Casuarina glauca Roots

text: phagemid

text: pBluescript


text: National University of Singapore

text: live

text: Generic

text: Pseudomonas aeruginosa-infected Carcinoscorpius rotundicauda suppression subtractive hybridization EST libraries. The library was constructed using pooled cDNAs from 3 and 6 hours post-infection time-points (tester) and naive (driver) horseshoe crabs for suppression subtractive hybridization.

text: Carcinoscorpius rotundicauda

text: Male

text: Hemocyte

characteristic: tissue-type

text: Suppression subtractive hybridization ESTs from Carcinoscorpius rotundicauda

characteristic: title

text: pGEM-T Easy (Promega)


characteristic: External Id

text: live

text: Generic

text: The library was constructed according Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. Denatured RNA was size fractionated on a 1% agarose gel.First strand cDNA synthesis was primed with oligo-dT primer containing a Not I site .Double strand cDNA was size selected according to mRNA size fraction, ligated with EcoR I adaptor, digested with NotI and then cloned directionally into pYX-Asc vector. The library tag sequence located between the Not I site and the polyA tail is AATAATTACG. This library was created for the University Iowa Brain Anatomy Project (BMAP): 'Gene Discovery in the Developing Mouse Nervous System', supported by National Institute of Mental Health (NIMH).

text: newborn (1, 5, 15 days )

text: DH10B (T1 phage resistant)

text: Eye

text: Mus musculus

text: EcoR I

text: Not I

text: whole eye

text: Plasmid

text: pYX-Asc

characteristic: vector


text: Cardiff University

characteristic: INSDC center name

text: live

text: Generic

text: The library was prepared using protocols given by the supplier (Stratagene).

text: Adult

characteristic: dev-stage

text: Lumbricus rubellus

text: Whole worm

text: Earthworm Atrazine Exposure library

text: pBluescript II SK+


text: Nara Institute of Science and Technology

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: 2-month-old plant

text: Nicotiana sylvestris

text: mixture of wounded and un-wounded leaf

text: partially normalized diploid tobacco cDNA library


text: Cold Spring Harbor Laboratory

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: DNA was extracted from Arabidopsis thaliana whole plant tissue, provided by members of Rob Martienssen's lab, using TRIZOL. Primers were designed in Hypothetical genes and un-annotated regions in Arabidopsis that are conserved in Brassica oleracea located in the short arm of chromosome 4. PCR products were either cloned into pCR TOPO 2.1 vector (Invitrogen) and then sequenced using -21 M13 forward and reverse universal primers or treated with Exonuclease 1 and Shrimp Alkaline phosphatase and sequenced using the specific primers.

text: Arabidopsis thaliana

text: Whole Plant

text: Arabidopsis RT-PCR Products (CSHL)


text: Kansas State University

text: live

text: Generic

text: mixed larvae or adults

text: Tribolium castaneum

characteristic: strain

text: mixed

text: Exelixis Tribolium castaneum cDNA Library

text: Plasmid

text: SPORT 1


text: NOAA Coastal Center for Environmental Health and Biomolecular Research

text: live

text: Generic

characteristic: NCBI submission package

text: Karenia brevis

text: Karenia brevis EST Library (L99-05)

text: Plasmid

text: LambdaZapII


text: Washington University School of Medicine

text: live

text: Generic

text: GoldRush

text: Total RNA was extracted separately from each stage [young fruitlet(<1cm), young fruitlet (1 cm dia.), young fruitlet (12cm dia.), maturing fruit I, maturing fruit II, mature fruit], using the 'pine tree' method. Poly(A)+mRNA was isolated twice from total RNA from each stage using the Oligotex Direct mRNA kit (Qiagen). mRNA was reverse transcribed into double stranded cDNA using a modified oligo18(dT) primer with an identifying tag sequence (see table below). cDNA's from different stages were pooled in equal amounts before adaptor ligation. Tag identification when sequencing from 5' end: Stage 1 (young fruitlet) insert 18(A)TCGTG; Stage 2 (young fruitlet 1cm dia) insert 18(A)TGCTG; Stage 3 (young fruitlet 12cm dia) insert 18(A)TCGGT; Stage 4 (maturing fruit I) insert 18(A)TGCGA; Stage 5 (maturing fruit II) insert 18(A)TCGGA; Stage 6 (mature fruit) insert 18(A)TGCGT; Tag identification when sequencing from 3' end: Stage 1 (young fruitlet) CACGA18(T) insert; Stage 2 (young fruitlet 1cm dia) CAGCA18(T) insert; Stage 3 (young fruitlet 12cm dia) ACCGA18(T) insert; Stage 4 (maturing fruit I) TCGCA18(T) insert; Stage 5 (maturing fruit II) TCCGA18(T) insert; Stage 6 (mature fruit) ACGCA18(T) insert. Double stranded cDNAs were size selected (more than 450 bp), adaptored with EcoRI adapters at both ends and then digested with NotI. The cDNAs were then directionally cloned into EcoR1-NotI digested pBS II SK(+) phagemid vector(Stratagene). Identification of adaptors and tags in 5'-end sequenced clones: (Vector)...TAAGCTT(End Vector)(Start EcoR1_adaptor)GATATCGAATTCCATTGTGTTGGG (End EcoR1_adaptor)(Start Insert)....AAAAAAAAAAAAAAAAAA(End Insert) (Start Tag)TGCGA(End Tag)(Start NotI_site/Vector)GCGGCCGCCACCGCGG... The total number of white colony forming units (cfu) in the primary library before amplification was 2.1x10^6 cfu (colony forming units). The background of empty clones was less than 1%. Inserts ranged from 0.5kb to 4 kb, as determined by PCR. Purified plasmid DNA from the primary library was converted to single-stranded circles and used as a template for PCR amplification using the T7 and T3 priming sites flanking the cloned cDNA inserts. The purified PCR products, representing the entire cloned cDNA population, were used as a driver for normalization. Hybridization between the single-stranded library and the PCR products was carried out for 44 hours at 30C. Unhybridized single-stranded DNA circles were separated from hybridized DNA rendered partially double-stranded and electroporated into DH10B cells to generate the normalized library. The total number of clones with insert was 5.6x10^6 cfu. Background of empty clones was less than 1%.

characteristic: description

text: DH10B ampicillin resistant

text: Malus domestica

text: NotI

text: Fruit

text: Mdfrt

text: Phagemid (ampicillin resistant)

text: pBluescript II SK (+)


text: Lawrence Berkeley National Lab

text: live

text: Generic

characteristic: cell-line

text: oligodT primed from LPS induced mbn2 cell line.

text: Drosophila melanogaster

characteristic: organism

text: NotI

text: XhoI

text: Exelixis FlyTag MN08 BlueScript

text: pBluescript


text: Lawrence Berkeley National Lab

text: live

text: Generic

text: RNA was isolated from fat body from 3rd instar larva challenged with gram+/- bacteria. cDNA was oligodT primed.

text: 3rd instar larva

text: fat body

text: Drosophila melanogaster

text: NotI

text: XhoI

text: Exelixis FlyTag ML01 pSport-Tag21


text: The University of Arizona

text: live

text: Generic

text: Library made by Invitrogen with RNA supplied by Wendle lab. Directional cloned into NotI-EV. Colonies plated/picked by AGI. More glycerol clones held in -80.

text: first true leaves

characteristic: lab-host

text: Gossypium raimondii

text: NotI

text: EcoRV

text: whole seedlings

text: Plasmid


text: The University of Arizona

text: live

text: Generic

text: _3 to +3 DPA

text: Gossypium raimondii

text: NotI

text: EcoRV

text: floral


text: Nantong Medical college

text: live

text: Generic

text: Gekko japonicus

text: brain and spinal cord

text: brain and spinal cord cDNA library in Gecko


text: USDA/ARS, Southern Regional Research Center

text: live

text: Generic

text: mycelia

characteristic: cell type

text: This normalized cDNA expression library was constructed using a mixture of mycelial cells grown under eight different medium conditions and harvested at 5 time points (18, 24, 48, 72, 96 hours). The poly-A sequence was trimmed off before ligating to vector.

text: developmental stages from 18 to 96 hours

text: E. coli DH10B T1 resistant cells

text: Aspergillus flavus

text: NotI, at the 5 prime end

text: EcoRI, at the 3 prime end

text: asexual mycelia

characteristic: sex

text: NRRL 3357

text: Aspergillus flavus Normalized cDNA Expression Library

text: Plasmid

text: pBlueScript (SK+) (Stratagene), antibiotic selection marker: Carbenicillin


text: University of Edinburgh

text: live

text: Generic

text: mixed

text: Eurydice pulchra

text: mixed

text: Eurydice pulchra TT library

characteristic: title


characteristic: External Id

text: live

text: Generic

text: third instar larva

text: Hydropsyche sp. T20

text: silk gland

characteristic: tissue-type

text: Hydropsyche sp. T20 silk gland third instar larva


text: The University of Georgia, Department of Plant Biology

characteristic: INSDC center name

text: live

text: Generic

text: The library was prepared from polyA+ RNA from the roots of 1-year-old loblolly pine (Pinus taeda) cuttings that were rooted and then planted in washed sand. Prior to harvesting tissues for RNA isolation, the rooted cuttings were maintained for 27 days (April 2003) under ambient conditions in a local greenhouse. They were kept on a weekly regimen of 0.5x nutrient-complete Hoagland's solution and supplemented with additional water sufficient to maintain 5% soil moisture content. Pots holding the rooted cuttings were fully submerged in water for 24 hours prior to harvest of the roots for RNA isolation. Double-stranded cDNA was cloned unidirectionally into pSL1180. Inserts can be excised with EcoRI (5' end) and XhoI (3' end).

text: DH10B-T1 phage-resistant E. coli

text: root

characteristic: organ

text: Pinus taeda

text: EcoRI

text: XhoI

text: 3 CCLONES

text: Root flooded

characteristic: vector


characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Misgurnus anguillicaudatus

text: Misgurnus anguillicaudatus


text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Paramisgurnus dabryanus

text: Paramisgurnus dabryanus


text: live

text: Generic

characteristic: NCBI submission package

text: The library was prepared from polyA+ RNA from the needles of 1-year-old loblolly pine (Pinus taeda) cuttings that were rooted and then planted in washed sand. Just before harvesting needles for RNA isolation, the rooted cuttings were maintained for 27 days (April 2003) under ambient conditions in a local greenhouse. They were kept on a weekly regimen of 0.5x nutrient-complete Hoagland's solution and supplemented with additional water sufficient to maintain a 15% soil moisture content. Double-stranded cDNA was cloned unidirectionally into pSL1180. Inserts can be excised with EcoRI (5' end) and XhoI (3' end).

text: DH10B-T1 phage-resistant E. coli

text: needle

text: Pinus taeda

text: EcoRI

text: XhoI

text: 3 CCLONES

characteristic: strain

text: Needles control


text: live

text: Generic

text: The library was prepared from polyA+ RNA from the roots of 1-year-old loblolly pine (Pinus taeda)cuttings that were rooted and then planted in washed sand. The rooted cuttings were maintained for 27 days (April 2003 harvest) under ambient conditions in a local greenhouse. They were kept on a weekly regimen of 0.5x nutrient-complete Hoagland's solution and supplemented with additional water sufficient to maintain a 15% soil moisture content. Twenty-four hours (24 h) prior to harvesting roots for mRNA preparation, the potted trees were tipped 90 degree to lay the pots on their sides to induce a grivitropic response. Double-stranded cDNA was cloned unidirectionally into pSL1180. Inserts can be excised with EcoRI (5' end) and XhoI (3' end).

characteristic: description

text: DH10B-T1 phage-resistant E. coli

text: Pinus taeda

text: EcoRI

text: XhoI

text: 3 CCLONES

text: Root gravitropism April 2003 test


text: live

text: Generic

text: The library was prepared from polyA+ RNA from the roots of 1-year-old loblolly pine (Pinus taeda) cuttings that were rooted and then planted in washed sand. The rooted cuttings were maintained for 117 days (July 2003 harvest) under ambient conditions in a local greenhouse. They were kept on a weekly regimen of 0.5x nutrient-complete Hoagland's solution and supplemented with additional water sufficient to maintain a 15% soil moisture content. Double-stranded cDNA was cloned unidirectionally into pSL1180. Inserts can be excised with EcoRI (5' end) and XhoI (3' end).

text: DH10B-T1 phage-resistant E. coli

text: Root

text: Pinus taeda

characteristic: organism

text: EcoRI

text: XhoI

text: 3 CCLONES

text: Root control 2 (late)


text: live

text: Generic

text: The library was prepared from polyA+ RNA from the roots of 1-year-old loblolly pine (Pinus taeda) cuttings that were rooted and then planted in washed sand. The rooted cuttings were maintained for 207 days (October 2003 harvest) under ambient conditions in a local greenhouse. They were kept on a weekly regimen of 0.5x nutrient-complete Hoagland's solution and supplemented with additional water sufficient to maintain a 15% soil moisture content. Twenty-four hours (24 h) prior to harvesting roots for mRNA preparation, the potted trees were tipped 90 degree to lay the pots on their sides to induce a gravitropic response. Double-stranded cDNA was cloned unidirectionally into pSL1180. Inserts can be excised with EcoRI (5' end) and XhoI (3' end).

text: DH10B-T1 phage-resistant E. coli

characteristic: lab-host

text: Root

text: Pinus taeda

text: EcoRI

text: XhoI

text: 2 CCLONES

text: Root gravitropism October 2003 test


text: University of Edinburgh

text: live

text: Generic

text: The library was prepared by Wim Damen (Institute for Genetics,University of Cologne) and sequenced in Edinburgh.

text: Adult

characteristic: dev-stage

text: Cupiennius salei

text: EcoRI

text: XhoI

text: Phage

text: Lambda ZAP (stratagene)


text: University of British Columbia

text: live

text: Generic

characteristic: cultivar

text: mRNA was isolated from each tissue source independently and equal quantities of mRNA from each tissue were then pooled. cDNA was prepared from 5 micrograms of mRNA and directionally ligated into the pBluescript II SK (+) XR vector using the pBluescript II XR cDNA Library Construction Kit according to manufacturer's instructions with modifications (Stratagene). Plasmid DNA was then transformed by electroporation into DH10B cells (Invitrogen) for propagation. Normalization was applied according to published methods [Bonaldo M.F. et al. (1996) Genome Research 6(9):791] in order to reduce the abundance of highly expressed transcripts.

text: three year old clonal trees grown under greenhouse conditions in standard potting soil mixture.

text: E. coli DH10B cells

text: Roots

text: Picea sitchensis

text: EcoRI (5' end of cDNA)

text: XhoI (3' end of cDNA)

text: Hermaphrodite

characteristic: sex

text: Young root growth (terminal 1-3 cm) and old root growth (distal to terminal 1-3 cm) tissues

text: pBluescript II SK (+) XR


characteristic: External Id

text: University of British Columbia

text: live

text: Generic

text: mRNA was isolated from each tissue source independently and equal quantities of mRNA from each tissue were then pooled. cDNA was prepared from 5 micrograms of mRNA and directionally ligated into the pBluescript II SK (+) XR vector using the pBluescript II XR cDNA Library Construction Kit according to manufacturer's instructions with modifications (Stratagene). Plasmid DNA then was transformed by electroporation into DH10B cells (Invitrogen) for propagation. Normalization was applied according to published methods [Bonaldo M.F. et al. (1996) Genome Research 6(9):791] in order to reduce the abundance of the highly expressed transcripts.

text: mature trees harvested at Vernon, British Columbia in 2001

text: E. coli DH10B cells

text: Phloem

text: Picea glauca

text: EcoRI (5' end of cDNA)

text: XhoI (3' end of cDNA)

text: Hermaphrodite

text: Early season phloem harvested June 15th, mid season phloem harvested July 10th and late season phloem harvested August 17th

characteristic: title

text: pBluescript II SK (+) XR


text: National Cancer Institute / NIH

characteristic: INSDC center name

text: live

text: Generic

text: Library was prepared from 50 ug of total RNA by oligo-dT priming and AMV reverse transcriptase. After addition of EcoRI linkers and EcoRI-XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-4B columns and fractions containing cDNAs larger than 500 bp were ligated into EcoRI-XhoI-digested lambda ZAPII (UniZAP-XR) and packaged in vitro. Average insert size is 1.4 kb. The original library contained 6 x 106 recombinants, of which 3 x 106 were amplified and stored at -70 C in SM buffer containing 7% DMSO. 3 x 106 pfu were mass excised and the resulting phagemids used to infect Top10F. References: Science 253, 196-196 and Methods in Molecular Biology 97, 555-574. Additional sequences from this library have been deposited under the name Xenopus laevis dorsal blastopore lip. Library constructed by Bruce Blumberg (University of California, Irvine, Department of Developmental and Cell Biology).

text: embryo

text: Xenopus laevis

text: EcoRI

text: XhoI

text: both

text: dorsal blastopore lip

characteristic: tissue-type

text: Blumberg_Cho dorsal blastopore lip

text: phagemid

text: pBluescript SK-


text: National Cancer Institute / NIH

text: live

text: Generic

text: RNA obtained from female overies animals at 8 wk old. Tissues were snap-frozen and kept at -80C for two days before RNA extraction and purification (Tri-reagent method). cDNA was primed using oligo-dT primer: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3' and cloned into the EcoRV/NotI sites of pExpress-1. Size-selection >1.25kb resulted in an average insert size of 1.7kb. This primary library is not normalized (normalized library is NIH_MGC_252) and was constructed by Express Genomics (Frederick, MD). Note: this is a NIH_MGC library

text: DH10B TonA

text: ovary

characteristic: organ

text: Rattus norvegicus

text: NotI

text: NotI

text: Ovary, strain - Norway Line3. Age8 weeks. Tissue was snap-frozen adn transferred in -70C. RNAse free for the entire procedure

text: plasmid

characteristic: vector


text: National Cancer Institute / NIH

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: RNA obtained from female animals at 8 wk old. Tissues were snap-frozen and kept at -80C for two days before RNA extraction and purification (Tri-reagent method). cDNA was primed using oligo-dT primer: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3' and cloned into the EcoRV/NotI sites of pExpress-1. Size-selection >1.25kb resulted in an average insert size of 1.5 kb. This primary library is normalized (non-normalized primary library is NIH_MGC_252) and was constructed by Express Genomics (Frederick, MD). Note: this is a NIH_MGC library.

text: DH10B TonA

text: ovary

text: Rattus norvegicus

text: EcoRV

text: NotI

text: Ovary - Brown Norway Line 3 Age 8 weeks. Tissues were snap-frozen and transfered in -70c. RNAse free the entire procedure.

text: plasmid


text: Michigan State University

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Vlaspik

text: Six hours after challenged with Phytophthora capsici

text: Fruit 12 days after pollination

text: Fruit

text: Cucumis sativus

text: Female (gynoecious)

text: Cucumber fruit cDNA-AFLP fragment 12 days after pollination


text: Michigan State University

text: live

text: Generic

characteristic: NCBI submission package

text: Vlaspik

text: Differentially regulated from 2 to 12 days

text: Fruit 2 to 12 days after pollination

text: Fruit

text: Cucumis sativus

text: Female (gynoecious)

text: Cucumber fruit cDNA-AFLP fragment 2 to 12 days after pollination


text: live

text: Generic

text: R-gene-free potato material

text: 48h infected with Phytophthora infestans

characteristic: description

text: Solanum tuberosum

text: leaf

text: Solanum tuberosum leaf mRNA


text: Prince of Songkla University

text: live

text: Generic

text: Subtracted cDNA library constructed by Clontech PCR-SelectTM cDNA Subtraction Kit, directionally cloned into pGEM-T Easy vector.

text: Ovary

text: Penaeus merguiensis

characteristic: organism

text: EcoR I

text: Penaeus merguiensis Ovary cDNA library

text: pGEM-T Easy


text: Roslin Institute

text: live

text: Generic

text: Single pass sequencing; Normalised library constructed from pooled ovaries

text: Sus scrofa

text: EcoRI

text: NotI

text: ovary


text: Roslin Institute

text: live

text: Generic

text: Single pass sequencing. Normalised library constructed from pooled early embryos, from 8-cell stage to blastocysts.

text: Sus scrofa

text: EcoRI

text: NotI

text: embryo

text: pBlueScriptII(SK+)


text: Roslin Institute

text: live

text: Generic

text: Single pass sequencing. Normalised library constructed from pooled tissue from day 30 placentas.

text: Sus scrofa

text: EcoRI

text: NotI

text: placenta

text: pBlueScriptII(KS+)


text: Roslin Institute

text: live

text: Generic

text: Single pass sequencing. Normalised library constructed from Bovine Uterus tissue.

text: Bos taurus

text: EcoRI

text: NotI

text: uterus

text: pBluescriptII(SK+)


text: Roslin Institute

text: live

text: Generic

text: Single pass sequencing. Normalised library constructed from pig uterus.

text: Sus scrofa

text: EcoRI

text: NotI

text: uterus

text: pBlueScriptII(KS+)


text: Roslin Institute

text: live

text: Generic

text: Single pass sequencing. Normalised library constructed from bovine ovary.

text: Bos taurus

text: EcoRI

text: SmaI

text: ovary

text: pBlueScriptII(SK+)


text: University of Delhi South Campus

text: live

text: Generic

text: CPAN 1676

text: The cDNA library was constructed from polyA+ RNA by using the ZAP express cDNA synthesis kit, Stratagene, USA.

text: 4-day-old etiolated seedling

text: Triticum aestivum

text: EcoRI

text: XhoI

text: hermaphrodite

text: shoot

text: Wheat 4-day-old etiolated seedling shoot library

text: Phagemid

text: pBK-CMV


text: The University of Georgia, Department of Plant Biology

text: live

text: Generic

text: The library was prepared from polyA+ RNA from the needles of 1-year-old loblolly pine (Pinus taeda) cuttings that were rooted and then planted in washed sand. The rooted cuttings were maintained for 117 days (July 2003 harvest) under ambient conditions in a local greenhouse. They were kept on a weekly regimen of 0.5x nutrient-complete Hoagland's solution and supplemented with additional water sufficient to maintain a 15% soil moisture content. Needles were from the same trees used to prepare the RTCNT2 library. Double-stranded cDNA was cloned unidirectionally into pSL1180. Inserts can be excised with EcoRI (5' end) and XhoI (3' end).

text: DH10B-T1 phage-resistant E. coli

characteristic: lab-host

text: needles

text: Pinus taeda

text: EcoRI

text: XhoI

text: 3 CCLONES

characteristic: strain

text: Needles control 2


text: live

text: Generic

text: The library was prepared from polyA+ RNA from the roots of 1-year-old loblolly pine (Pinus taeda) cuttings that were rooted and then planted in washed sand. The rooted cuttings were maintained for 117 days (July 2003 harvest) under ambient conditions in a local greenhouse. They were kept on a weekly regimen of 0.5x nutrient-complete Hoagland's solution and supplemented with additional water sufficient to maintain a 15% soil moisture content. For twenty-eight days (28 d) prior to harvesting roots for mRNA preparation, the trees received Hoagland's solution lacking potassium (K) to induce a potassium-deficiency. Double-stranded cDNA was cloned unidirectionally into pSL1180. Inserts can be excised with EcoRI (5' end) and XhoI (3' end).

text: DH10B-T1 phage-resistant E. coli

text: Root

text: Pinus taeda

text: EcoRI

text: XhoI

text: 3 CCLONES

text: Roots minus potassium


text: Nanjing Medical University

text: live

text: Generic

text: adult

characteristic: dev-stage

text: Homo sapiens

text: male

text: testis

text: human testis cDNA Library


text: Institut National de la Recherche Agronomique

text: live

text: Generic

text: Colomer

characteristic: cultivar

text: mRNA extracted from leaf samples originating from apricot grafted scion

text: grafted scion

text: leaf

text: Prunus armeniaca

text: Apricot cv. Colomer cDNA library

text: plasmid

text: pGEM-T


characteristic: External Id

text: Washington University School of Medicine

text: live

text: Generic

text: pGEM-T Vector( from Promega) is ampicillin resistent, M13 forward and M13 reverse, or SP6 and T7 primers should be used for sequencing

text: Hydra vulgaris

text: EcoRI

text: EcoRI

text: Hydra EST -Kiel 4

text: plasmid

text: pGEM-T


text: Hawaii Agriculture Research Center

text: live

text: Generic

text: Partial cDNAs cloned from BTH treated seedlings, after suppression-subtraction hybridization (SSH) with cDNA from H2O treated seedlings

text: 3 month old seedlings

text: Carica papaya

text: kapoho

text: leaves

text: Papaya BTH-induced ESTs in PCRII-TOPO

characteristic: title

text: PCRII-TOPO


text: Yantai Research Center of Scientific Technology of Life

characteristic: INSDC center name

text: live

text: Generic

text: Homo sapiens

text: human epididymidis


text: Kazusa DNA Research Institute

text: live

text: Generic

text: The cDNA library was made from a mixture of cells grown under various conditions

text: Chlamydomonas reinhardtii

text: EcoRI

text: XhoI

text: Chlamydomonas reinhardtii C9 various conditions

text: pBluescriptII SK-


text: University of Padova

text: live

text: Generic

text: caucasian

text: Homo sapiens

text: bone marrow

characteristic: tissue-type


text: University of Padova

text: live

text: Generic

text: caucasian

text: Homo sapiens

text: heart


text: Cardiff University

text: live

text: Generic

text: Lumbricus rubellus

text: Mixed

characteristic: sex

text: Earthworm Head Enriched library


text: ENEA

text: live

text: Generic

text: Queen Elisa

text: Red fruit

text: E. coli

text: Fruit

characteristic: organ

text: Fragaria x ananassa

text: EcoRI

text: XhoI

text: Strawberry Lambda Zap Express Library

text: Phage

text: Uni-ZAP XR lambda ZAP

characteristic: vector


text: Institute of Arable Crop Research

characteristic: INSDC first public

characteristic: INSDC last update

text: live

text: Generic

text: Mercia

text: 3-4 days post anthesis

text: Triticum aestivum

text: carpel tissue


text: Institute of Arable Crop Research

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Mercia

text: 24 hours post anthesis

text: Triticum aestivum

text: carpel tissue


text: Institute of Arable Crop Research

text: live

text: Generic

characteristic: NCBI submission package

text: Mercia

text: 6 days post anthesis

text: Triticum aestivum

text: embryo (micropylar tip)


text: Institute of Arable Crop Research

text: live

text: Generic

text: Mercia

text: 8 days post anthesis

text: Triticum aestivum

characteristic: organism

text: endosperm


text: Institute of Arable Crop Research

text: live

text: Generic

text: Mercia

text: 8 days post anthesis

text: Triticum aestivum

text: embryo (micropylar tip)


text: Institute of Arable Crop Research

text: live

text: Generic

text: Mercia

text: 10 days post anthesis

text: Triticum aestivum

text: embryo (micropylar tip)


text: Institute of Arable Crop Research

text: live

text: Generic

text: Mercia

text: 10 days post anthesis

text: Triticum aestivum

text: endosperm


text: Institute of Arable Crop Research

text: live

text: Generic

text: Mercia

text: 6 days post anthesis

text: Triticum aestivum

text: maternal tissue (minus endosperm and embryo)


text: Institute of Arable Crop Research

text: live

text: Generic

text: Mercia

text: 8 days post anthesis

text: Triticum aestivum


text: Institute of Arable Crop Research

text: live

text: Generic

text: Mercia

text: 14 days post anthesis

text: Triticum aestivum

text: endosperm


text: National Cancer Institute / NIH

text: live

text: Generic

text: RNA obtained from brain tissue of 8 wk old animal. Tissues were snap-frozen and kept at -80C before RNA extraction and purification (Tri-reagent method). cDNA was primed using oligo-dT primer: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3' and cloned into the EcoRV/NotI sites of pExpress-1. Size-selection >1.25kb resulted in an average insert size of 2.18 kb. This primary library is not normalized (normalized library is NIH_MGC_255) and was constructed by Express Genomics (Frederick, MD). Note: this is a NIH_MGC library

characteristic: description

text: DH10B TonA

characteristic: lab-host

text: brain/CNS

text: Rattus norvegicus

text: EcoRV

text: NotI

text: both

text: Brain - Pooled from several tissues from one or more individuals

text: plasmid


text: National Cancer Institute / NIH

text: live

text: Generic

text: RNA obtained from brain tissue of 8 wk old animal. Tissues were snap-frozen and kept at -80C before RNA extraction and purification (Tri-reagent method). cDNA was primed using oligo-dT primer: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3' and cloned into the EcoRV/NotI sites of pExpress-1. Size-selection >1.25kb resulted in an average insert size of 1.7 kb. This primary library is a normalized (primary library is NIH_MGC_254) and was constructed by Express Genomics (Frederick, MD). Note: this is a NIH_MGC library

text: DH10B TonA

text: brain/CNS

text: Rattus norvegicus

text: EcoRV

text: NotI

text: both

text: plasmid


text: live

text: Generic

text: The cDNA library was constructed by Keliang Tang, and Robert Cole at Washington University. Total RNA was converted to cDNA using the template-switching PCR method (Creator SMART cDNA, Clontech Inc.). First strand was PCR amplified using the same primer set and the fragments were digested with SfiI. The fragments were size selected, ligated into vector pDNR_LIB containing directional SfiI sites, and electroporated into GC10 competent cells. WARNING: the library contains a small percentage of cDNAs derived from the human host cells.

text: Tachyzoite

characteristic: dev-stage

text: GC10 Competent Cells(PGC)

text: Toxoplasma gondii

text: SfiI

text: SfiI

text: Tg COUG Tachyzoite cDNA Library

text: pDNR-LIB


text: Huazhong agricultural university of China

text: live

text: Generic

text: Sus scrofa

text: longissimus dorsi muscle

text: Sus scrofa longissimus dorsi muscle


text: National Institute of Health

text: live

text: Generic

text: B16 melanoma BL6 cells

characteristic: cell type

text: Mus musculus

characteristic: strain

text: Skin

text: B16 melanoma BL6 cells RT-PCR


characteristic: External Id

text: RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH

text: live

text: Generic

text: Rattus norvegicus

text: Rat pBluescript Lion

characteristic: title


characteristic: INSDC center name

text: live

text: Generic

text: Mus musculus

text: Mouse pBluescript Lion


text: The Royal Veterinary and Agricultural University

text: live

text: Generic

text: Sus scrofa

text: small intestine

characteristic: tissue-type

text: Sus scrofa small intestine


text: Monash University

text: live

text: Generic

text: roots germinated in vitro; country: Australia:South East

text: 8 day old seedlings

text: Eucalyptus globulus subsp. globulus

text: Eucalyptus globulus subsp. globulus 8 day old seedlings


text: Metapontum Agrobios

text: live

text: Generic

text: Ofanto

characteristic: cultivar

text: 20 days

text: Triticum turgidum subsp. durum

text: durum

characteristic: sub species

text: etiolated seedling

text: Triticum turgidum subsp. durum etiolated seedling 20 days


text: Metapontum Agrobios

characteristic: INSDC first public

text: live

text: Generic

text: 20 days old

text: Triticum turgidum

text: etiolated seedling

text: Triticum turgidum etiolated seedling 20 days old


text: INRA

characteristic: INSDC last update

text: live

text: Generic

text: Clone distribution : AGENAE Resource centre. Francois PIUMI, Francois.Piumi@jouy.inra.fr, INRA, CEA Radiobiologie et Etude du genome (LREG), Domaine de Vilvert, 78352, Jouy-en-Josas cedex, FRANCE, +33 (0) 1.34.65.28.02, +33 (0) 1.34.65.22.73

text: embryon

text: Bos taurus

text: early elongating embryos (+/- 15 mm)

text: Day 14 bovine embryos (bcai)

text: pCR2.1 (Invitrogen)

characteristic: vector


text: INRA

text: live

characteristic: INSDC status

text: Generic

characteristic: NCBI submission model

text: Tagged oligo-dT was used to identify the origin of the ESTs where possible. (A18)TGCGT = extraembryonic tissue; (A18)TCGGT = 36-day oldfetus; (A18)TGCGA = 64-day old fetus (organs); (A18)TCGGA = 64-day old fetus (body)

text: Bos taurus

text: embryo (day 36 and day 64), extra-embryonic tissue (day 14 to 25)

text: Normalized and Subtracted bovine embryonic and extraembryonic tissues (bcaz)


text: Chulalongkorn University

text: live

text: Generic

characteristic: NCBI submission package

text: The cDNA library was generated as part of Penaeus monodon EST project. Total RNA was isolated from hemocyte of Penaeus monodon. The mRNA was further purified, reverse transcribed and cloned to Lambda ZAPII.

text: Penaeus monodon

characteristic: organism

text: Not I

text: Sal I

text: Hemocyte

text: Hemocyte normal library

text: Phage

text: Lambda ZAP II


text: ESALQ - University of Sao Paulo

text: live

text: Generic

text: This cDNA library was constructed with the SuperScript Plasmid System with Gateway Tecnology kit (Invitrogen), following manufacture's protocols. Plasmid DNA was purified using a modified alkaline lysis method. Sequencing reactions were conducted using the kit Big Dye Terminator Cycle Sequencing Ready Reaction (Applied Biosystems) according to the manufacturer's recommendations. Clones were sequenced by the 5' end with T7 primer. Sequencing reactions were analyzed on ABI Prism 3100 Genetic Analyzer (Applied Biosystems). The quality and clustering of the ESTs were analyzed using the softwares Phred/Cap3. Only EST sequences with Phred quality greater than 20 and at least 150 bp were considered for clustering.

characteristic: description

text: 21 days old

characteristic: lab-host

text: Gallus gallus

text: NotI

text: SalI

text: pituitary and hypothalamus

text: Plasmid


text: live

text: Generic

text: The library was constructed according Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. Denatured RNA was size fractionated on a 1% agarose gel.First strand cDNA synthesis was primed with oligo-dT primer containing a Not I site .Double strand cDNA was size selected according to mRNA size fraction, ligated with EcoR I adaptor, digested with NotI and then cloned directionally into pYX-Asc vector. The library tag sequence located between the Not I site and the polyA tail is AATAATTACG. This library was created for the University Iowa Brain Anatomy Project (BMAP): 'Gene Discovery in the Developing Mouse Nervous System', supported by National Institute of Mental Health (NIMH).

text: newborn (1, 5, 15 days) and embryonic (15, 16, 17, 18 dpc)

characteristic: dev-stage

text: DH10B (T1 phage resistant)

text: Eye

characteristic: organ

text: Mus musculus

text: EcoR I

text: Not I

text: whole eye

text: Plasmid

text: pYX-Asc


characteristic: External Id

text: Universite de Montreal

text: live

text: Generic

text: Monosiga ovata

text: M-1 (ATCC 50635)

characteristic: strain

text: Monosiga ovata Lambda Zap Express Library

text: lambda ZAPII


text: MIPS

characteristic: INSDC center name

text: live

text: Generic

text: adult

text: Homo sapiens

text: NotI

text: SalI

text: breast cancer

text: synonym: hmcf1

characteristic: title


text: University of Heidelberg

text: live

text: Generic

text: neural

characteristic: cell type

text: Library was obtained from a differential display experiment comparing ventral midbrain of E12 En1-/-En2-/-, E12 En1+/-En2-/-, E14 En1+/-En2-/- and wild type adult olfactory bulb

text: E12 and E14

text: Mus musculus

text: C57/BL6 presumed

text: ventral midbrain

characteristic: tissue-type

text: Ventral midbrain engrailed mutants

text: Plasmid

text: Bluescript II SK(-)


text: National University of Singapore

characteristic: INSDC first public

text: live

text: Generic

text: Library was prepared by Aaron Chen Angus and Goh Sock Yong, National University of Singapore, Singapore.

text: Glycyphagus domesticus

text: Glycyphagus domesticus cDNA library


text: University of Illinois at Urbana-Champaign

characteristic: INSDC last update

text: live

text: Generic

text: The cDNA library was constructed, normalized and subtracted by Dr. A. Hernandez, W.M. Keck Center, University of Illinois Urbana-Champaign and S. Degrelle, BDR, Institut National de la Recherche Agronomique, France, as described in Bonaldo, M.F., Lennon, G. and Soares, M.B. (1996),Genome Research 6(9): 791-806. This clone is from library BE03, a normalized library of embryonic tissues subtracted with 7800 known placental EST sequences. The double stranded cDNA was size selected (more than 450 bp), adaptored with EcoRI adaptors at both ends and then digested with NotI. The cDNA was then directionally cloned into EcoRI-NotI digested pGEM11Zf(+) phagemid vector. Insert size was between 450-2200 bp. Sequencing was done from the 5' end of the clone. Tagged oligo-dT was used to identify the source of the ESTs where possible. (A18)TGCGT = extraembryonic tissue; (A18)TCGGT = 36-day old fetus; (A18)TGCGA = 64-day old fetus (organs); (A18)TCGGA = 64-day old fetus (body)

text: Bos taurus

text: EcoRI

text: NotI

text: embryo (day 36 and day 64) and extra-embryonic tissue (day 14 to 25) Normalized and subtracted library

text: BE03 Normalized and Subtracted bovine embryonic and extraembryonic tissue


text: live

text: Generic

text: The cDNA library was constructed, normalized and subtracted by Dr. A. Hernandez, W.M. Keck Center, University of Illinois Urbana-Champaign and S. Degrelle, BDR, Institut National de la Recherche Agronomique, France, as described in Bonaldo, M.F., Lennon, G. and Soares, M.B. (1996),Genome Research 6(9): 791-806. This clone is from library BE04, a normalized library of embryonic tissues subtracted with 7800 known placental EST sequences and 5000 clones from the BE03 library. The double stranded cDNA was size selected (more than 450 bp), adaptored with EcoRI adaptors at both ends and then digested with NotI. The cDNA was then directionally cloned into EcoRI-NotI digested pGEM11Zf(+) phagemid vector. Insert size was between 450-2200 bp. Sequencing was done from the 5' end of the clone. Tagged oligo-dT was used to identify the source of the ESTs where possible. (A18)TGCGT = extraembryonic tissue; (A18)TCGGT = 36-day old fetus; (A18)TGCGA = 64-day old fetus (organs); (A18)TCGGA = 64-day old fetus (body)

text: Bos taurus

text: EcoRI

text: NotI

text: embryo (day 36 and day 64) and extra-embryonic tissue (day 14 to 25) Normalized and twice subtracted library

text: BE04 Normalized and Subtracted bovine embryonic and extraembryonic tissue

characteristic: vector


text: Tokyo Institute of Technology

text: live

characteristic: INSDC status

text: Generic

text: Oryzias latipes

text: regenerating fin

text: Oryzias latipes regenerating fin 3dpa


text: Tokyo Institute of Technology

text: live

text: Generic

characteristic: NCBI submission model

text: Oryzias latipes

text: regenerating fin

text: Oryzias latipes regenerating fin 10dpa


text: The Royal Veterinary and Agricultural University

text: live

text: Generic

characteristic: NCBI submission package

text: adult

text: Sus scrofa

text: small intestine

text: Sus scrofa small intestine LambdaZapII

text: LambdaZapII


text: Royal School for Veterinary Studies

text: live

text: Generic

text: macrophage

text: Sus scrofa

characteristic: organism

text: muscle

text: muscle - muscle minus alveolar macrophage


text: Royal School for Veterinary Studies

text: live

text: Generic

text: macrophage

text: Sus scrofa

text: lung

text: forward - stimulated minus unstimulated macrophage


text: Royal School for Veterinary Studies

text: live

text: Generic

text: macrophage

text: Sus scrofa

text: lung

text: reverse - unstimulated minus stimulated macrophage


text: Royal School for Veterinary Studies

text: live

text: Generic

text: macrophage

text: Sus scrofa

text: lung

text: uninfected - uninfected minus ASFV infected macrophage


text: Royal School for Veterinary Studies

text: live

text: Generic

text: macrophage

text: Sus scrofa

text: lung

text: infected - ASFV infected minus uninfected macrophage


text: University of Newcastle-upon-Tyne

text: live

text: Generic

text: Homo sapiens

text: Homo sapiens library (Tonkin ET)


text: Garvan Institute of Medical Research

text: live

text: Generic

text: Homo sapiens

text: Lymphocyte RACE ready cDNA


text: University of Helsinki

text: live

text: Generic

text: country: Israel:Ein Avdat

characteristic: description

text: adult

text: Populus euphratica

text: leaf

text: Populus euphratica leaf adult


text: University of Helsinki

text: live

text: Generic

text: country: China:Xinjiang

text: 3-6 months

text: Populus euphratica

text: leaf

text: Populus euphratica leaf 3-6 months


text: University of Helsinki

text: live

text: Generic

text: country: China:Xinjiang

text: in vitro plantlet

text: Populus euphratica

text: shoot

text: Populus euphratica shoot in vitro plantlet China


text: University of Helsinki

text: live

text: Generic

text: country: Israel:Ein Avdat

text: in vitro plantlet

text: Populus euphratica

text: shoot

text: Populus euphratica shoot in vitro plantlet Israel


text: University of Helsinki

text: live

text: Generic

text: country: China:Xinjiang

text: 3-6 months

text: Populus euphratica

text: root

text: Populus euphratica root 3-6 months


text: University of Helsinki

text: live

text: Generic

text: country: China:Xinjiang

text: 3-6 months

text: Populus euphratica

text: shoot

text: Populus euphratica shoot 3-6 months


text: University of Helsinki

text: live

text: Generic

text: country: China:Xinjiang

text: 3-6 months

text: Populus euphratica

text: cambium

text: Populus euphratica cambium 3-6 months


text: University of Bologna

text: live

text: Generic

text: country: Italy

text: adult

text: Sus scrofa

text: skeletal muscle, biceps femoris

text: Sus scrofa skeletal muscle, biceps femoris adult


text: Institut Gustave-Roussy

text: live

text: Generic

text: epithelial

characteristic: cell-line

text: Homo sapiens

text: colon

text: Homo sapiens SW613-S


text: Kobe University Graduate School of Medicine

text: live

text: Generic

text: adult

characteristic: dev-stage

text: Mus musculus

text: male

characteristic: sex

text: pancratic islet

text: mouse (C57BL/6) pancreatic islet library with recombination-based method