text: public

characteristic: StrainOrLine

tag: Namespace:Imperial College IoSSB Sequencing Facility

text: ArrayExpress

characteristic: broker name

text: Protocols: Blumeria graminis f. sp. hordei (strain DH14) was maintained on barley (Hordeum vulgare) cv Golden Promise in an isolated, growth chamber maintained at 25C and a 16 hour light/ 8 hour dark cycle. The barley seedlings (10 to 12 day old) were inoculated by dusting conidia from heavily infected plants. Conidia were collected by vacuum aspiration onto Millipore filters (HATF02500) and frozen at -80C. Epiphytic structures were dissected by dipping infected leaves in a cellulose acetate (5%) dissolved in acetone; the acetone was left to dry off and the epiphytic material embedded in the cellulose acetate was dissected and snap frozen in liquid nitrogen and stored at -80C. Infected abaxial epidermis was collected by dissection, after removal of the epiphytic material, and stored at -80C. Primary barley leaves infected with B. graminis 5 days after infection were dissected to separate the epiphytic structures (samples E-1 to -3: surface runner hyphae, appressoria, condiophore foot cells, conidiophores and conidia, Fig. 1G) from the infected epidermis (samples H-1 to -3 containing haustoria). Three fully independent biological replicates where sampled. RNA was extracted from conidia and dissected material collected and ground in liquid nitrogen as described above. RNA was extracted as previously described by M. Both et al., Mol Plant-Microbe Interact 18, 125 (2005) and further purified using RNAClean XP beads (Beckman Coulter, Brea, CA, USA).

text: Blumeria graminis

text: Blumeria graminis

text: epiphytic

characteristic: organism part

text: Blumeria graminis


status: PUBLIC

tag: attribute

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Imperial College IoSSB Sequencing Facility

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: description

text: Blumeria graminis

characteristic: organism

text: Blumeria graminis

text: haustoria

text: Blumeria graminis

characteristic: title

type: has member


text: public

text: ArrayExpress

text: Blumeria graminis

text: Blumeria graminis

text: epiphytic

text: Blumeria graminis


Triticum aestivum cv. Chinese spring

text: public

text: Triticum aestivum cv. Chinese spring

text: bread wheat

characteristic: common name

text: Chinese spring

characteristic: cultivar

text: Genomic DNA was isolated from purified nuclei based on modification of an existing protocol (Peterson et al, Construction of Plant Bacterial Artificial Chromosome (BAC) Libraries: An Illustrated Guide. Second edition, 2002). A modified sucrose buffer SEB (Sucrose-based Extraction Buffer: 10% v/v TKE, 500 mM sucrose, 4 mM spermidine, 1 mM spermine tetrahydrochloride, 1.2g/L PEG 8000, 0.13% w/v sodium diethyldithiocarbamate and 0.2% v/v ß-mercaptoethanol) was used instead of the recommended MEB buffer, which gave poor quality gDNA in wheat. 60-80g frozen leaf material was ground in liquid nitrogen and added to 1 litre SEB, and the protocol followed. Typically 200-500ug of high quality gDNA was extracted using this method.

text: line 42

characteristic: line

text: Triticum aestivum

text: single seed descent line from original Sears euploid line

characteristic: origin

text: Triticum aestivum

text: Triticum aestivum cv. Chinese spring


Triticum aestivum Savannah variety

text: public

text: Triticum aestivum Savannah variety

text: bread wheat

text: Triticum aestivum

text: Triticum aestivum

text: Triticum aestivum, Savannah variety


Triticum aestivum Rialto variety

text: public

text: Triticum aestivum Rialto variety

text: bread wheat

text: Triticum aestivum

text: Triticum aestivum

text: Triticum aestivum, Rialto variety


Triticum aestivum Avalon variety

text: public

text: Triticum aestivum Avalon variety

text: bread wheat

text: Triticum aestivum

text: Triticum aestivum

text: Triticum aestivum, Avalon variety


text: CCME-COLORADO

text: public

characteristic: age

characteristic: age in years

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characteristic: altitude

characteristic: anonymized name

text: n

text: UBERON:feces

characteristic: body habitat

text: UBERON:feces

characteristic: body product

text: UBERON:feces

characteristic: body site

text: human gut metagenome

characteristic: depth

text: Human gut microbiome differentiation viewed across cultures, ages and families

characteristic: elevation

text: ENVO:human-associated habitat

characteristic: env biome

text: ENVO:human-associated habitat

characteristic: env feature

text: ENVO:feces

characteristic: env matter

text: n

text: GAZ:Venezuela

characteristic: geographic location (country and/or sea)

text: human

characteristic: host common name

characteristic: host subject id

characteristic: host taxid

characteristic: latitude

characteristic: longitude

text: y

characteristic: newilluminadatagenerated

text: human gut metagenome

text: y

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text: CCME-COLORADO

characteristic: INSDC center name

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characteristic: INSDC status

characteristic: SRA accession

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characteristic: body product

text: UBERON:feces

characteristic: body site

text: human gut metagenome

characteristic: common name

characteristic: depth

characteristic: description

characteristic: elevation

text: ENVO:human-associated habitat

characteristic: env biome

text: ENVO:human-associated habitat

text: ENVO:feces

characteristic: family relationship

text: n

text: GAZ:United States of America

text: human

text: y

text: human gut metagenome

characteristic: organism

text: y

text: 0.01, g

text: human gut metagenome

characteristic: title


characteristic: ENA-FIRST-PUBLIC

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characteristic: External Id

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characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: n

text: UBERON:feces

text: UBERON:feces

text: UBERON:feces

text: human gut metagenome

text: ENVO:human-associated habitat

text: ENVO:human-associated habitat

characteristic: env feature

text: ENVO:feces

characteristic: env matter

text: Mother

text: n

text: GAZ:Venezuela

characteristic: geographic location (country and/or sea)

text: human

characteristic: host common name

characteristic: host subject id

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characteristic: latitude

characteristic: longitude

text: y

characteristic: newilluminadatagenerated

text: human gut metagenome

text: y

characteristic: public

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characteristic: run lane

text: 0.01, g

characteristic: sample id

characteristic: sample name

text: human gut metagenome

characteristic: sex

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characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: CCME-COLORADO

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

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text: n

text: UBERON:feces

characteristic: body habitat

text: UBERON:feces

characteristic: body product

text: UBERON:feces

characteristic: body site

text: human gut metagenome

characteristic: common name

characteristic: depth

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characteristic: elevation

text: ENVO:human-associated habitat

characteristic: env biome

text: ENVO:human-associated habitat

text: ENVO:feces

text: Mother

characteristic: family relationship

text: n

text: GAZ:Malawi

text: human

text: y

text: human gut metagenome

characteristic: organism

text: y

text: 0.01, g

text: human gut metagenome

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: CCME-COLORADO

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: n

text: UBERON:feces

text: UBERON:feces

text: UBERON:feces

text: human gut metagenome

text: ENVO:human-associated habitat

text: ENVO:human-associated habitat

characteristic: env feature

text: ENVO:feces

characteristic: env matter

text: Grandfather

text: n

text: GAZ:Venezuela

characteristic: geographic location (country and/or sea)

text: human

characteristic: host common name

characteristic: host subject id

characteristic: host taxid

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characteristic: longitude

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text: human gut metagenome

text: y

characteristic: public

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text: human gut metagenome

characteristic: sex

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characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: CCME-COLORADO

characteristic: INSDC center name

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characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

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characteristic: anonymized name

text: n

text: UBERON:feces

characteristic: body habitat

text: UBERON:feces

characteristic: body product

text: UBERON:feces

characteristic: body site

text: human gut metagenome

characteristic: common name

characteristic: depth

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text: ENVO:human-associated habitat

characteristic: env biome

text: ENVO:human-associated habitat

text: ENVO:feces

characteristic: family relationship

text: n

text: GAZ:Malawi

text: human

text: y

text: human gut metagenome

characteristic: organism

text: y

text: 0.01, g

text: human gut metagenome

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: CCME-COLORADO

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: n

text: UBERON:feces

text: UBERON:feces

text: UBERON:feces

text: human gut metagenome

text: ENVO:human-associated habitat

text: ENVO:human-associated habitat

characteristic: env feature

text: ENVO:feces

characteristic: env matter

text: Father

text: n

text: GAZ:United States of America

characteristic: geographic location (country and/or sea)

text: human

characteristic: host common name

characteristic: host subject id

characteristic: host taxid

characteristic: latitude

characteristic: longitude

text: y

characteristic: newilluminadatagenerated

text: human gut metagenome

text: y

characteristic: public

characteristic: run

characteristic: run lane

text: 0.01, g

characteristic: sample id

characteristic: sample name

text: human gut metagenome

characteristic: sex

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characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: CCME-COLORADO

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

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characteristic: age in years

characteristic: age unit

characteristic: altitude

characteristic: anonymized name

text: n

text: UBERON:feces

characteristic: body habitat

text: UBERON:feces

characteristic: body product

text: UBERON:feces

characteristic: body site

text: human gut metagenome

characteristic: common name

characteristic: depth

characteristic: description

characteristic: elevation

text: ENVO:human-associated habitat

characteristic: env biome

text: ENVO:human-associated habitat

text: ENVO:feces

characteristic: family relationship

text: n

text: GAZ:United States of America

text: human

text: y

text: human gut metagenome

characteristic: organism

text: y

text: 0.01, g

text: human gut metagenome

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: CCME-COLORADO

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: n

text: UBERON:feces

text: UBERON:feces

text: UBERON:feces

text: human gut metagenome

text: ENVO:human-associated habitat

text: ENVO:human-associated habitat

characteristic: env feature

text: ENVO:feces

characteristic: env matter

text: Mother

text: n

text: GAZ:United States of America

characteristic: geographic location (country and/or sea)

text: human

characteristic: host common name

characteristic: host subject id

characteristic: host taxid

characteristic: latitude

characteristic: longitude

text: y

characteristic: newilluminadatagenerated

text: human gut metagenome

text: y

characteristic: public

characteristic: run

characteristic: run lane

text: 0.01, g

characteristic: sample id

characteristic: sample name

text: human gut metagenome

characteristic: sex

characteristic: taxon id


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: CCME-COLORADO

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

characteristic: age in years

characteristic: age unit

characteristic: altitude

characteristic: anonymized name

text: n

text: UBERON:feces

characteristic: body habitat

text: UBERON:feces

characteristic: body product

text: UBERON:feces

characteristic: body site

text: human gut metagenome

characteristic: common name

characteristic: depth

characteristic: description

characteristic: elevation

text: ENVO:human-associated habitat

characteristic: env biome

text: ENVO:human-associated habitat

text: ENVO:feces

characteristic: family relationship

text: n

text: GAZ:Malawi

text: human

text: y

text: human gut metagenome

characteristic: organism

text: y

text: 0.01, g

text: human gut metagenome

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: CCME-COLORADO

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: n

text: UBERON:feces

text: UBERON:feces

text: UBERON:feces

text: human gut metagenome

text: ENVO:human-associated habitat

text: ENVO:human-associated habitat

characteristic: env feature

text: ENVO:feces

characteristic: env matter

text: Mother

text: n

text: GAZ:United States of America

characteristic: geographic location (country and/or sea)

text: human

characteristic: host common name

characteristic: host subject id

characteristic: host taxid

characteristic: latitude

characteristic: longitude

text: y

characteristic: newilluminadatagenerated

text: human gut metagenome

text: y

characteristic: public

characteristic: run

characteristic: run lane

text: 0.01, g

characteristic: sample id

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text: human gut metagenome

characteristic: sex

characteristic: taxon id


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: CCME-COLORADO

characteristic: INSDC center name

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text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

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text: n

text: UBERON:feces

characteristic: body habitat

text: UBERON:feces

characteristic: body product

text: UBERON:feces

characteristic: body site

text: human gut metagenome

characteristic: common name

characteristic: depth

characteristic: description

characteristic: elevation

text: ENVO:human-associated habitat

characteristic: env biome

text: ENVO:human-associated habitat

text: ENVO:feces

text: Child

characteristic: family relationship

text: n

text: GAZ:United States of America

text: human

text: y

text: human gut metagenome

characteristic: organism

text: y

text: 0.01, g

text: human gut metagenome

characteristic: title


characteristic: ENA-FIRST-PUBLIC

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characteristic: External Id

text: CCME-COLORADO

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text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: n

text: UBERON:feces

text: UBERON:feces

text: UBERON:feces

text: human gut metagenome

text: ENVO:human-associated habitat

text: ENVO:human-associated habitat

characteristic: env feature

text: ENVO:feces

characteristic: env matter

text: Father

text: n

text: GAZ:United States of America

characteristic: geographic location (country and/or sea)

text: human

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text: human gut metagenome

text: y

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text: 0.01, g

characteristic: sample id

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text: human gut metagenome

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text: CCME-COLORADO

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text: public

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text: n

text: UBERON:feces

characteristic: body habitat

text: UBERON:feces

characteristic: body product

text: UBERON:feces

characteristic: body site

text: human gut metagenome

characteristic: common name

characteristic: depth

characteristic: description

characteristic: elevation

text: ENVO:human-associated habitat

characteristic: env biome

text: ENVO:human-associated habitat

text: ENVO:feces

text: Sibling

characteristic: family relationship

text: n

text: GAZ:Malawi

text: human

text: y

text: human gut metagenome

characteristic: organism

text: y

text: 0.01, g

text: human gut metagenome

characteristic: title


characteristic: ENA-FIRST-PUBLIC

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text: CCME-COLORADO

characteristic: INSDC center name

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text: n

text: UBERON:feces

text: UBERON:feces

text: UBERON:feces

text: human gut metagenome

text: ENVO:human-associated habitat

text: ENVO:human-associated habitat

characteristic: env feature

text: ENVO:feces

characteristic: env matter

text: n

text: GAZ:Venezuela

characteristic: geographic location (country and/or sea)

text: human

characteristic: host common name

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text: human gut metagenome

text: y

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characteristic: sample id

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text: human gut metagenome

characteristic: sex

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text: CCME-COLORADO

characteristic: INSDC center name

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text: n

text: UBERON:feces

characteristic: body habitat

text: UBERON:feces

characteristic: body product

text: UBERON:feces

characteristic: body site

text: human gut metagenome

characteristic: common name

characteristic: depth

characteristic: description

characteristic: elevation

text: ENVO:human-associated habitat

characteristic: env biome

text: ENVO:human-associated habitat

text: ENVO:feces

text: Sibling

characteristic: family relationship

text: n

text: GAZ:Malawi

text: human

text: y

text: human gut metagenome

characteristic: organism

text: y

text: 0.01, g

text: human gut metagenome

characteristic: title


characteristic: ENA-FIRST-PUBLIC

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text: CCME-COLORADO

characteristic: INSDC center name

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characteristic: age

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text: n

text: UBERON:feces

text: UBERON:feces

text: UBERON:feces

text: human gut metagenome

text: ENVO:human-associated habitat

text: ENVO:human-associated habitat

characteristic: env feature

text: ENVO:feces

characteristic: env matter

text: y

text: GAZ:United States of America

characteristic: geographic location (country and/or sea)

text: human

characteristic: host common name

characteristic: host subject id

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characteristic: latitude

characteristic: longitude

text: y

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text: human gut metagenome

text: y

characteristic: public

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text: 0.01, g

characteristic: sample id

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text: human gut metagenome

characteristic: sex

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characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: CCME-COLORADO

characteristic: INSDC center name

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text: public

characteristic: INSDC status

characteristic: SRA accession

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text: n

text: UBERON:feces

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text: UBERON:feces

characteristic: body product

text: UBERON:feces

characteristic: body site

text: human gut metagenome

characteristic: common name

characteristic: depth

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characteristic: elevation

text: ENVO:human-associated habitat

characteristic: env biome

text: ENVO:human-associated habitat

text: ENVO:feces

text: Mother

characteristic: family relationship

text: n

text: GAZ:Malawi

text: human

text: y

text: human gut metagenome

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text: y

text: 0.01, g

text: human gut metagenome

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text: CCME-COLORADO

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characteristic: Submitter Id

text: n

text: UBERON:feces

text: UBERON:feces

text: UBERON:feces

text: human gut metagenome

text: ENVO:human-associated habitat

text: ENVO:human-associated habitat

characteristic: env feature

text: ENVO:feces

characteristic: env matter

text: Mother

text: y

text: GAZ:United States of America

characteristic: geographic location (country and/or sea)

text: human

characteristic: host common name

characteristic: host subject id

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characteristic: latitude

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text: y

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text: human gut metagenome

text: y

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text: 0.01, g

characteristic: sample id

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text: human gut metagenome

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characteristic: ENA-LAST-UPDATE

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text: CCME-COLORADO

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text: public

characteristic: INSDC status

characteristic: SRA accession

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text: n

text: UBERON:feces

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text: UBERON:feces

characteristic: body product

text: UBERON:feces

characteristic: body site

text: human gut metagenome

characteristic: common name

characteristic: depth

characteristic: description

characteristic: elevation

text: ENVO:human-associated habitat

characteristic: env biome

text: ENVO:human-associated habitat

text: ENVO:feces

characteristic: family relationship

text: n

text: GAZ:United States of America

text: human

text: y

text: human gut metagenome

characteristic: organism

text: y

text: 0.01, g

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