characteristic: Chlorophyll Sensor

text: tbd

characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: GSC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (TARA_N000002454) was collected during the Tara Oceans expedition (2009-2013) at station TARA_133 (latitudeN=35.4135, longitudeE=-127.7053) on date/time=2011-10-19T02:27, using a PLANKTON NET, TYPE=Single, MESH_Size_(�m)=5, MOUTH_Opening_(m^2)=0.196349, LENGTH_(m)=2.5. The sample material (particulate matter, including plankton (ENVO:xxxxxxxx)) was collected at a depth of 43-47 m, targeting a deep chlorophyll maximum layer (ENVO:xxxxxxxx) in the marine biome (ENVO:00000447). The sample was size-fractionated (5-20 micrometres), and stored in liquid nitrogen for later detection of unicellular eukaryote (protist) nucleic acid sequences by pyrosequencing methods, and for later metagenomics analysis. This sample has replicate sample(s): TARA_N000002455.

characteristic: description

text: marine biome (ENVO:00000447)

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: water

text: tbd

text: marine metagenome

characteristic: organism

text: Tara Oceans expedition (2009-2013)

text: SV Tara

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: TARA_20111019T0227Z_133_EVENT_NET_N1_D_(45 m)_PROT_NUC-DNA(1L)_N5-20_TARA_N000002454

characteristic: title


status: PUBLIC

unit: mg Chl/m3

tag: attribute

characteristic: Chlorophyll Sensor

text: tbd

characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: GSC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

unit: DD

unit: practical salinity unit

unit: m

text: This sample (TARA_N000002175) was collected during the Tara Oceans expedition (2009-2013) at station TARA_135 (latitudeN=33.008, longitudeE=-121.7579) on date/time=2011-10-23T16:15, using a PLANKTON NET, TYPE=Bongo, MESH_Size_(�m)=180, MOUTH_Opening_(m^2)=0.258319, LENGTH_(m)=3. The sample material (particulate matter, including plankton (ENVO:xxxxxxxx)) was collected at a depth of 3-7 m, targeting a surface water layer (ENVO:00002042) in the marine biome (ENVO:00000447). The sample was size-fractionated (180-2000 micrometres), and stored in liquid nitrogen for later detection of unicellular eukaryote (protist) nucleic acid sequences by pyrosequencing methods, and for later metagenomics analysis. This sample has replicate sample(s): TARA_N000002176.

text: marine biome (ENVO:00000447)

text: surface water layer (ENVO:00002042)

text: water

characteristic: environmental package

characteristic: event date/time start

characteristic: event label

text: tbd

characteristic: further details

characteristic: last update date

characteristic: latitude start

characteristic: longitude start

text: (search radius=5 degrees) Marine Ecoregion:Northern California (MRGID:21844), Marine Ecoregion:Southern California Bight (MRGID:22001)

characteristic: marine region

unit: µmol/L

characteristic: nitrate sensor

text: marine metagenome

unit: µmol/kg

characteristic: oxygen sensor

text: Tara Oceans expedition (2009-2013)

characteristic: project name

characteristic: protocol label

characteristic: sample collection device

text: This version can be used to provide data discovery services

characteristic: sample status

characteristic: sampling campaign

text: SV Tara

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

unit: ºC

text: TARA_20111023T1615Z_135_EVENT_NET_N1+N2_S_(5 m)_PROT_NUC-DNA(1L)_N180-2000_TARA_N000002175


characteristic: Chlorophyll Sensor

text: tbd

characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: GSC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (TARA_N000000864) was collected during the Tara Oceans expedition (2009-2013) at station TARA_076 (latitudeN=-20.9724, longitudeE=-35.2724) on date/time=2010-10-16T12:45, using a PLANKTON NET, TYPE=Single, MESH_Size_(�m)=5, MOUTH_Opening_(m^2)=0.196349, LENGTH_(m)=2.5. The sample material (particulate matter, including plankton (ENVO:xxxxxxxx)) was collected at a depth of 3-7 m, targeting a surface water layer (ENVO:00002042) in the marine biome (ENVO:00000447). The sample was size-fractionated (5-20 micrometres), and stored in liquid nitrogen for later detection of unicellular eukaryote (protist) nucleic acid sequences by pyrosequencing methods, and for later metagenomics/transcriptomics analysis. This sample has replicate sample(s): TARA_N000000863,TARA_N000000865,TARA_N000000866.

characteristic: description

text: marine biome (ENVO:00000447)

characteristic: environment (biome)

text: surface water layer (ENVO:00002042)

characteristic: environment (feature)

characteristic: environment (material)

text: water

characteristic: environmental package

text: tbd

text: (search radius=5 degrees) Marine Ecoregion:Trindade and Martin Vaz Islands (MRGID:21989), Marine Ecoregion:Eastern Brazil (MRGID:21990)

text: marine metagenome

characteristic: organism

text: Tara Oceans expedition (2009-2013)

text: SV Tara

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: TARA_20101016T1245Z_076_EVENT_NET_N1_S_(5 m)_PROT_NUC-RNA(1L)_N5-20_TARA_N000000864

characteristic: title


characteristic: Chlorophyll Sensor

text: tbd

characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: GSC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: This sample (TARA_N000001267) was collected during the Tara Oceans expedition (2009-2013) at station TARA_095 (latitudeN=-31.409, longitudeE=-93.9464) on date/time=2011-03-21T16:44, using a PLANKTON NET, TYPE=Bongo, MESH_Size_(�m)=180, MOUTH_Opening_(m^2)=0.258319, LENGTH_(m)=3. The sample material (particulate matter, including plankton (ENVO:xxxxxxxx)) was collected at a depth of 3-7 m, targeting a surface water layer (ENVO:00002042) in the marine biome (ENVO:00000447). The sample was size-fractionated (180-2000 micrometres), and stored in liquid nitrogen for later detection of unicellular eukaryote (protist) nucleic acid sequences by pyrosequencing methods, and for later metagenomics analysis.

text: marine biome (ENVO:00000447)

text: surface water layer (ENVO:00002042)

text: water

characteristic: event date/time start

characteristic: event label

text: tbd

characteristic: further details

characteristic: last update date

characteristic: latitude start

characteristic: longitude start

characteristic: nitrate sensor

text: marine metagenome

characteristic: oxygen sensor

text: Tara Oceans expedition (2009-2013)

characteristic: project name

characteristic: protocol label

characteristic: sample collection device

characteristic: sample status

characteristic: sampling campaign

text: SV Tara

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

text: TARA_20110321T1644Z_095_EVENT_NET_N1+N2_S_(5 m)_PROT_NUC-DNA(1L)_N180-2000_TARA_N000001267


characteristic: Chlorophyll Sensor

text: tbd

characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: GSC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (TARA_N000002428) was collected during the Tara Oceans expedition (2009-2013) at station TARA_133 (latitudeN=35.4155, longitudeE=-127.7409) on date/time=2011-10-18T19:38, using a PLANKTON NET, TYPE=Bongo, MESH_Size_(�m)=180, MOUTH_Opening_(m^2)=0.258319, LENGTH_(m)=3. The sample material (particulate matter, including plankton (ENVO:xxxxxxxx)) was collected at a depth of 3-7 m, targeting a surface water layer (ENVO:00002042) in the marine biome (ENVO:00000447). The sample was size-fractionated (180-2000 micrometres), and stored in liquid nitrogen for later detection of unicellular eukaryote (protist) nucleic acid sequences by pyrosequencing methods, and for later metagenomics analysis. This sample has replicate sample(s): TARA_N000002429.

characteristic: description

text: marine biome (ENVO:00000447)

characteristic: environment (biome)

text: surface water layer (ENVO:00002042)

characteristic: environment (feature)

characteristic: environment (material)

text: water

characteristic: environmental package

text: tbd

text: marine metagenome

characteristic: organism

text: Tara Oceans expedition (2009-2013)

text: SV Tara

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: TARA_20111018T1938Z_133_EVENT_NET_N1+N2_S_(5 m)_PROT_NUC-DNA(1L)_N180-2000_TARA_N000002428

characteristic: title


characteristic: Chlorophyll Sensor

text: tbd

characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: GSC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: This sample (TARA_N000002193) was collected during the Tara Oceans expedition (2009-2013) at station TARA_135 (latitudeN=33.02, longitudeE=-121.7406) on date/time=2011-10-23T18:03, using a PLANKTON NET, TYPE=Double, MESH_Size_(�m)=20, MOUTH_Opening_(m^2)=0.192442, LENGTH_(m)=2.5. The sample material (particulate matter, including plankton (ENVO:xxxxxxxx)) was collected at a depth of 3-7 m, targeting a surface water layer (ENVO:00002042) in the marine biome (ENVO:00000447). The sample was size-fractionated (20-180 micrometres), and stored in liquid nitrogen for later detection of unicellular eukaryote (protist) nucleic acid sequences by pyrosequencing methods, and for later metagenomics analysis. This sample has replicate sample(s): TARA_N000002194.

text: marine biome (ENVO:00000447)

text: surface water layer (ENVO:00002042)

text: water

characteristic: event date/time start

characteristic: event label

text: tbd

characteristic: further details

characteristic: last update date

characteristic: latitude start

characteristic: longitude start

characteristic: marine region

characteristic: nitrate sensor

text: marine metagenome

characteristic: oxygen sensor

text: Tara Oceans expedition (2009-2013)

characteristic: project name

characteristic: protocol label

characteristic: sample collection device

characteristic: sample status

characteristic: sampling campaign

text: SV Tara

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

text: TARA_20111023T1803Z_135_EVENT_NET_N1+N2_S_(5 m)_PROT_NUC-DNA(1L)_N20-180_TARA_N000002193


characteristic: Chlorophyll Sensor

text: tbd

characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: GSC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (TARA_N000003039) was collected during the Tara Oceans expedition (2009-2013) at station TARA_139 (latitudeN=6.4808, longitudeE=-94.9704) on date/time=2011-12-15T15:25, using a PLANKTON NET, TYPE=Single, MESH_Size_(�m)=5, MOUTH_Opening_(m^2)=0.196349, LENGTH_(m)=2.5. The sample material (particulate matter, including plankton (ENVO:xxxxxxxx)) was collected at a depth of 3-7 m, targeting a surface water layer (ENVO:00002042) in the marine biome (ENVO:00000447). The sample was size-fractionated (5-20 micrometres), and stored in liquid nitrogen for later detection of unicellular eukaryote (protist) nucleic acid sequences by pyrosequencing methods, and for later metagenomics analysis. This sample has replicate sample(s): TARA_N000003040.

characteristic: description

text: marine biome (ENVO:00000447)

characteristic: environment (biome)

text: surface water layer (ENVO:00002042)

characteristic: environment (feature)

characteristic: environment (material)

text: water

characteristic: environmental package

text: tbd

text: (search radius=5 degrees) Marine Province:Tropical East Pacific (MRGID:21769), Marine Ecoregion:Northern Galapagos Islands (MRGID:21871)

text: marine metagenome

characteristic: organism

text: Tara Oceans expedition (2009-2013)

text: SV Tara

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: TARA_20111215T1525Z_139_EVENT_NET_N1_S_(5 m)_PROT_NUC-DNA(1L)_N5-20_TARA_N000003039

characteristic: title


characteristic: Chlorophyll Sensor

text: tbd

characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: GSC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: This sample (TARA_N000002199) was collected during the Tara Oceans expedition (2009-2013) at station TARA_135 (latitudeN=32.9916, longitudeE=-121.8536) on date/time=2011-10-24T00:15, using a PLANKTON NET, TYPE=Bongo, MESH_Size_(�m)=180, MOUTH_Opening_(m^2)=0.258319, LENGTH_(m)=3. The sample material (particulate matter, including plankton (ENVO:xxxxxxxx)) was collected at a depth of 28-32 m, targeting a deep chlorophyll maximum layer (ENVO:xxxxxxxx) in the marine biome (ENVO:00000447). The sample was size-fractionated (180-2000 micrometres), and stored in liquid nitrogen for later detection of unicellular eukaryote (protist) nucleic acid sequences by pyrosequencing methods, and for later metagenomics analysis. This sample has replicate sample(s): TARA_N000002200.

text: marine biome (ENVO:00000447)

text: water

characteristic: event date/time start

characteristic: event label

text: tbd

characteristic: further details

characteristic: last update date

characteristic: latitude start

characteristic: longitude start

characteristic: marine region

characteristic: nitrate sensor

text: marine metagenome

characteristic: oxygen sensor

text: Tara Oceans expedition (2009-2013)

characteristic: project name

characteristic: protocol label

characteristic: sample collection device

characteristic: sample status

characteristic: sampling campaign

text: SV Tara

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

text: TARA_20111024T0015Z_135_EVENT_NET_N1+N2_D_(30 m)_PROT_NUC-DNA(1L)_N180-2000_TARA_N000002199


characteristic: Chlorophyll Sensor

text: tbd

characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: GSC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (TARA_N000002195) was collected during the Tara Oceans expedition (2009-2013) at station TARA_135 (latitudeN=32.9909, longitudeE=-121.8522) on date/time=2011-10-24T00:34, using a PLANKTON NET, TYPE=Double, MESH_Size_(�m)=20, MOUTH_Opening_(m^2)=0.192442, LENGTH_(m)=2.5. The sample material (particulate matter, including plankton (ENVO:xxxxxxxx)) was collected at a depth of 28-32 m, targeting a deep chlorophyll maximum layer (ENVO:xxxxxxxx) in the marine biome (ENVO:00000447). The sample was size-fractionated (20-180 micrometres), and stored in liquid nitrogen for later detection of unicellular eukaryote (protist) nucleic acid sequences by pyrosequencing methods, and for later metagenomics analysis. This sample has replicate sample(s): TARA_N000002196.

characteristic: description

text: marine biome (ENVO:00000447)

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

text: water

characteristic: environmental package

text: tbd

text: (search radius=5 degrees) Marine Ecoregion:Northern California (MRGID:21844), Marine Ecoregion:Southern California Bight (MRGID:22001)

text: marine metagenome

characteristic: organism

text: Tara Oceans expedition (2009-2013)

text: SV Tara

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: TARA_20111024T0034Z_135_EVENT_NET_N1+N2_D_(30 m)_PROT_NUC-DNA(1L)_N20-180_TARA_N000002195

characteristic: title


characteristic: Chlorophyll Sensor

text: tbd

characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: GSC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: This sample (TARA_N000002187) was collected during the Tara Oceans expedition (2009-2013) at station TARA_135 (latitudeN=32.9903, longitudeE=-121.8463) on date/time=2011-10-24T01:37, using a PLANKTON NET, TYPE=Single, MESH_Size_(�m)=5, MOUTH_Opening_(m^2)=0.196349, LENGTH_(m)=2.5. The sample material (particulate matter, including plankton (ENVO:xxxxxxxx)) was collected at a depth of 28-32 m, targeting a deep chlorophyll maximum layer (ENVO:xxxxxxxx) in the marine biome (ENVO:00000447). The sample was size-fractionated (5-20 micrometres), and stored in liquid nitrogen for later detection of unicellular eukaryote (protist) nucleic acid sequences by pyrosequencing methods, and for later metagenomics analysis. This sample has replicate sample(s): TARA_N000002188.

text: marine biome (ENVO:00000447)

text: water

characteristic: event date/time start

characteristic: event label

text: tbd

characteristic: further details

characteristic: last update date

characteristic: latitude start

characteristic: longitude start

characteristic: marine region

characteristic: nitrate sensor

text: marine metagenome

characteristic: oxygen sensor

text: Tara Oceans expedition (2009-2013)

characteristic: project name

characteristic: protocol label

characteristic: sample collection device

text: This version can be used to provide data discovery services

characteristic: sample status

characteristic: sampling campaign

text: SV Tara

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

text: TARA_20111024T0137Z_135_EVENT_NET_N1_D_(30 m)_PROT_NUC-DNA(1L)_N5-20_TARA_N000002187


E-MTAB-2851:Sample 2

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Tromso

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-2851:Sample 2

tag: Namespace:University of Tromso

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: Protocols: Mono-cultures of either M. viscosa or A. wodanis in one tube was implanted in the abdomen of the fish. Or, M. viscosa and A. wodanis were implanted together in separate tubes in the abdomen of the fish RNA was isolated using the MasterPure™ Complete DNA and RNA Purification Kit (EPICENTER, Cat. Nos. MC85200 and MC89010) according to manufactures instructions. The single-stranded cDNA libraries were constructed as described in http://nar.oxfordjournals.org/content/37/22/e148.long

characteristic: description

text: In vivo, fish implant, Co-infection

characteristic: growth condition

text: Aliivibrio wodanis

characteristic: organism

text: Aliivibrio wodanis

text: Aliivibrio wodanis

characteristic: strain

text: Sample 2

characteristic: title

type: has member


E-MTAB-2851:Sample 1

text: University of Tromso

text: public

text: E-MTAB-2851:Sample 1

text: ArrayExpress

text: In vivo, fish implant, Single infection

text: Aliivibrio wodanis

text: Aliivibrio wodanis

text: Aliivibrio wodanis

text: Sample 1


E-MTAB-2851:Sample 3

text: University of Tromso

text: public

text: E-MTAB-2851:Sample 3

text: ArrayExpress

text: In vivo, fish implant, Single infection

text: Moritella viscosa

text: Moritella viscosa

text: Moritella viscosa

text: Sample 3


E-MTAB-2851:Sample 4

text: University of Tromso

text: public

text: E-MTAB-2851:Sample 4

text: ArrayExpress

text: In vivo, fish implant, Co-infection

text: Moritella viscosa

text: Moritella viscosa

text: Moritella viscosa

text: Sample 4


text: WUR

text: public

text: Netherlands

characteristic: geographic location (country and/or sea)

text: Homo Sapiens

characteristic: host

text: Romboutsia sp. Frifi

text: Romboutsia sp. Frifi

text: FRIFI

text: Genome sequence of Romboutsia str. Frifi


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

tag: Namespace:MRC CLINICAL SCIENCES CENTRE, IMPERIAL COLLEGE LONDON, DU CANE ROAD, LONDON W12 0NN, UK

text: Resequencing of MG1655 strain from Krisko lab stock

text: Escherichia coli str. K-12 substr. MG1655

characteristic: sub strain

text: MG1655 lab ancestor


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: MRC CLINICAL SCIENCES CENTRE, IMPERIAL COLLEGE LONDON, DU CANE ROAD, LONDON W12 0NN, UK

characteristic: INSDC center name

text: public

text: Resequencing of MG1655-derived mutH deletion strain evolved for ~500 generations

text: evolved mutH deletion strain


text: NTU

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Campylobacter sp.

text: Campylobacter sp.

text: C. jejuni_857


text: NTU

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: Campylobacter sp.

text: Campylobacter sp.

text: C. jejuni_5491


text: NTU

text: public

text: Campylobacter sp.

text: Campylobacter sp.

text: C. jejuni_623


text: NTU

text: public

text: Campylobacter sp.

text: Campylobacter sp.

text: C. jejuni_304


text: NTU

text: public

text: Campylobacter sp.

text: Campylobacter sp.

text: C. jejuni_484


text: NTU

text: public

text: Campylobacter sp.

text: Campylobacter sp.

text: C. jejuni_444


text: NTU

text: public

text: Campylobacter sp.

text: Campylobacter sp.

text: C. coli_99321


text: NTU

text: public

text: Campylobacter sp.

text: Campylobacter sp.

text: C. coli_03121


text: NTU

text: public

text: Campylobacter sp.

text: Campylobacter sp.

text: C. coli_03317


text: NTU

text: public

text: Campylobacter sp.

text: Campylobacter sp.

text: C. coli_03103


text: UB

text: public

text: Salmonella bongori extraction using CAMBIO kit dilution series 1 of 10

characteristic: description

text: Salmonella bongori

text: Salmonella bongori


text: UB

text: public

text: Salmonella bongori extraction using CAMBIO kit dilution series 2 of 10

text: Salmonella bongori

characteristic: organism

text: Salmonella bongori


text: UB

text: public

text: Salmonella bongori extraction using CAMBIO kit dilution series 3 of 10

text: Salmonella bongori

text: Salmonella bongori


text: UB

text: public

text: Salmonella bongori extraction using CAMBIO kit dilution series 4 of 10

text: Salmonella bongori

text: Salmonella bongori


text: UB

text: public

text: Salmonella bongori extraction using CAMBIO kit dilution series 5 of 10

text: Salmonella bongori

text: Salmonella bongori


text: UB

text: public

text: Salmonella bongori extraction using CAMBIO kit dilution series 6 of 10

text: Salmonella bongori

text: Salmonella bongori


text: UB

text: public

text: Salmonella bongori extraction using CAMBIO kit dilution series 7 of 10

text: Salmonella bongori

text: Salmonella bongori


text: UB

text: public

text: Salmonella bongori extraction using CAMBIO kit dilution series 8 of 10

text: Salmonella bongori

text: Salmonella bongori


text: UB

text: public

text: Salmonella bongori extraction using CAMBIO kit dilution series 9 of 10

text: Salmonella bongori

text: Salmonella bongori


text: UB

text: public

text: Salmonella bongori extraction using CAMBIO kit dilution series 10 of 10

text: Salmonella bongori

text: Salmonella bongori


text: UB

text: public

text: Salmonella bongori extraction using MP BIO kit dilution series 1 of 10

text: Salmonella bongori

text: Salmonella bongori


text: UB

text: public

text: Salmonella bongori extraction using MP BIO kit dilution series 2 of 10

text: Salmonella bongori

text: Salmonella bongori


text: UB

text: public

text: Salmonella bongori extraction using MP BIO kit dilution series 3 of 10

text: Salmonella bongori

text: Salmonella bongori


text: UB

text: public

text: Salmonella bongori extraction using MP BIO kit dilution series 4 of 10

text: Salmonella bongori

text: Salmonella bongori


text: UB

text: public

text: Salmonella bongori extraction using MP BIO kit dilution series 5 of 10

text: Salmonella bongori

text: Salmonella bongori


text: UB

text: public

text: Salmonella bongori extraction using MP BIO kit dilution series 6 of 10

text: Salmonella bongori

text: Salmonella bongori


text: UB

text: public

text: Salmonella bongori extraction using MP BIO kit dilution series 7 of 10

text: Salmonella bongori

text: Salmonella bongori


text: UB

text: public

text: Salmonella bongori extraction using MP BIO kit dilution series 8 of 10

text: Salmonella bongori

text: Salmonella bongori


text: UB

text: public

text: Salmonella bongori extraction using MP BIO kit dilution series 9 of 10

text: Salmonella bongori

text: Salmonella bongori


text: UB

text: public

text: Salmonella bongori extraction using MP BIO kit dilution series 10 of 10

text: Salmonella bongori

text: Salmonella bongori


text: UB

text: public

text: Salmonella bongori extraction using QIAGEN kit dilution series 1 of 10

text: Salmonella bongori

text: Salmonella bongori


text: UB

text: public

text: Salmonella bongori extraction using QIAGEN kit dilution series 2 of 10

text: Salmonella bongori

text: Salmonella bongori


text: UB

text: public

text: Salmonella bongori extraction using QIAGEN kit dilution series 3 of 10

text: Salmonella bongori

text: Salmonella bongori


text: UB

text: public

text: Salmonella bongori extraction using QIAGEN kit dilution series 4 of 10

text: Salmonella bongori

text: Salmonella bongori


text: UB

text: public

text: Salmonella bongori extraction using QIAGEN kit dilution series 5 of 10

text: Salmonella bongori

text: Salmonella bongori


text: UB

text: public

text: Salmonella bongori extraction using QIAGEN kit dilution series 6 of 10

text: Salmonella bongori

text: Salmonella bongori


text: UB

text: public

text: Salmonella bongori extraction using QIAGEN kit dilution series 7 of 10

text: Salmonella bongori

text: Salmonella bongori


text: UB

text: public

text: Salmonella bongori extraction using QIAGEN kit dilution series 8 of 10

text: Salmonella bongori

text: Salmonella bongori


text: UB

text: public

text: Salmonella bongori extraction using QIAGEN kit dilution series 9 of 10

text: Salmonella bongori

text: Salmonella bongori


text: UB

text: public

text: Salmonella bongori extraction using QIAGEN kit dilution series 10 of 10

text: Salmonella bongori

text: Salmonella bongori


text: UB

text: public

text: Salmonella bongori extraction using PSP PLUS kit dilution series 1 of 4

text: Salmonella bongori

text: Salmonella bongori


text: UB

text: public

text: Salmonella bongori extraction using PSP PLUS kit dilution series 2 of 4

text: Salmonella bongori

text: Salmonella bongori


text: UB

text: public

text: Salmonella bongori extraction using PSP PLUS kit dilution series 3 of 4

text: Salmonella bongori

text: Salmonella bongori


text: UB

text: public

text: Salmonella bongori extraction using PSP PLUS kit dilution series 4 of 4

text: Salmonella bongori

text: Salmonella bongori


Water

text: UB

text: public

text: Water

text: Negative water control

text: Salmonella bongori

text: Salmonella bongori

text: Water


text: PRI

text: public

text: S. pennellii LA716 contigs

text: Solanum pennellii

text: Solanum pennellii

text: S. pennellii LA716 contigs


MVIS

text: University of Tromso

text: public

text: MVIS

text: The complete genome sequence and annotation of Moritella viscosa 06/09/139

text: Moritella viscosa

text: Moritella viscosa

text: Moritella viscosa complete genome


text: University of Tromso

text: public

text: The complete genome sequence and annotation of Aliivibrio wodanis 06/09/139

text: Aliivibrio wodanis

text: Aliivibrio wodanis

text: Aliivibrio wodanis complete genome


text: Universite d'Auvergne

text: public

tag: Namespace:Universite d'Auvergne

text: Annotated genome of klebsiella pneumoniae LM21

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae

text: klebsiella pneumoniae LM21 genome


text: The University of York

text: public

tag: Namespace:The University of York

characteristic: collection date

text: type strain of Rhizobium lentis sp. nov.

text: cropland

characteristic: environment (biome)

text: field

characteristic: environment (feature)

text: root nodule

characteristic: environment (material)

text: plant-associated

characteristic: environmental package

characteristic: estimated size

text: Draft, 27X. 140 contigs

characteristic: finishing strategy

text: Bangladesh

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Natore, Bagatipara

characteristic: geographic location (region and locality)

text: lentil

characteristic: host common name

characteristic: host taxid

characteristic: investigation type

characteristic: isolation and growth condition

text: Rhizobium sp. BLR27

text: Bangladesh lentil rhizobia

characteristic: project name

characteristic: reference for biomaterial

text: aerobe

characteristic: relationship to oxygen

text: Rhizobium sp. BLR27

text: Ion Torrent PGM

characteristic: sequencing method

text: strain BLR27

characteristic: source material identifiers

characteristic: subspecific genetic lineage

text: Bangladesh lentil rhizobia


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: The University of York

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: type strain of Rhizobium bangladeshense sp. nov.

characteristic: description

text: cropland

characteristic: environment (biome)

text: field

characteristic: environment (feature)

text: root nodule

characteristic: environment (material)

text: plant-associated

text: Draft, 49X, 89 contigs

text: Bangladesh

characteristic: geographic location (country and/or sea)

text: Khulna, Mohammadpur

text: lentil

text: Rhizobium sp. BLR175

characteristic: organism

text: Bangladesh lentil rhizobia

text: aerobe

text: Rhizobium sp. BLR175

text: Ion Torrent PGM

text: strain BLR175

text: Bangladesh lentil rhizobia

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: The University of York

text: public

text: type strain of Rhizobium binae sp. nov.

text: cropland

text: field

text: root nodule

text: plant-associated

characteristic: environmental package

characteristic: estimated size

text: Draft, 45X, 187 contigs

characteristic: finishing strategy

text: Bangladesh

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Feni, Sarishadi

characteristic: geographic location (region and locality)

text: lentil

characteristic: host common name

characteristic: host taxid

characteristic: investigation type

characteristic: isolation and growth condition

text: Rhizobium sp. BLR195

text: Bangladesh lentil rhizobia

characteristic: project name

characteristic: reference for biomaterial

text: aerobe

characteristic: relationship to oxygen

text: Rhizobium sp. BLR195

text: Ion Torrent PGM

characteristic: sequencing method

text: strain BLR27

characteristic: source material identifiers

characteristic: subspecific genetic lineage

text: Bangladesh lentil rhizobia


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Universite d'Auvergne

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Annotated genome of Klebsiella pneumoniae LM21

characteristic: description

text: Klebsiella pneumoniae

characteristic: organism

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae LM21

characteristic: title


PP

text: FRIEDRICH SCHILLER UNIVERSITY JENA

text: public

text: PP

tag: Namespace:FRIEDRICH SCHILLER UNIVERSITY JENA

text: plus

text: Parasitella parasitica

text: Parasitella parasitica

text: CBS 412.66

characteristic: strain

text: NGI-315 ade-

text: Genome sequence of Parasitella parasitica


text: FRIEDRICH SCHILLER UNIVERSITY JENA

text: public

text: plus

text: Parasitella parasitica

text: Parasitella parasitica

text: CBS 412.66

text: NGI-315 ade-


text: Genoscope CEA

text: public

tag: Namespace:Genoscope CEA

text: Candidatus Hamiltonella defensa (Bemisia tabaci)

text: Prokaryotic genome sequencing


text: Vrije Universiteit Brussel

text: public

tag: Namespace:Vrije Universiteit Brussel

text: Acetobacter ghanensis

text: Acetobacter ghanensis

text: LMG 23848T

text: Acetobacter ghanensis 23848T


text: Vrije Universiteit Brussel

text: public

text: Acetobacter senegalensis

text: Acetobacter senegalensis

text: Acetobacter senegalensis 108B


text: public

tag: Namespace:Sars International Centre for Marine Molecular Biology

text: ArrayExpress

characteristic: broker name

text: Protocols: Adult Sycon ciliatum specimens were collected from fjords located near Bergen, Norway, and transported to laboratory in sea water collected at the place of their growth, with temperature between 14 and 18 degrees Celsius. They were gradually (1-3 hours) acclimatized to ambient temperature, avoiding temperatures exceeding 20 degrees. Field-collected water was gradually exchanged for sterile filtered natural sea water by replacing 1/4th to 1/3rd parts with the filtered water every 20-30 minutes 3-4 times. With aid of dissecting microscope, the samples were separated from the algae and cleaned from macroscopic contaminations by gently blowing water at them. Young syconoid sponges were immediately processed for RNA-isolation by placing in the lysis buffer from the AllPrep DNA/RNA Mini Kit (Qiagen). To obtain larvae, sponges containing larvae were kept in plastic cups with a pellet of 1-Hexadecanol (to avoid trapping and damage of the larvae by surface tension) until the larvae were released (1-4days). The larvae swimming near the surface were poured into clean Petri dishes. For RNA isolation, the larvae were kept in the petri dishes, including a pellet of 1-Hexadecanol, until settlement and metamorphosis, which took 6-10 days to complete, and which was periodically monitored under dissecting microscope. Once majority of specimens reached the targeted stage of metamorphosis, specimens in significantly different stage of development were removed and the remaining specimens were used for RNA isolation. Total RNA was isolated using the AllPrep DNA/RNA Mini Kit (Qiagen) according to manufacturers instructions. As the juvenile sponges were attached to the bottom of the petri dishes, the water was replaced with the lysis buffer and juveniles scraped of the bottom using cell culture scraper before transferring to 1.5 ml tubes and disruption. Isolated RNA was analysed on agarose gels and quantified by Nanodrop, some samples were additionally tested using Bioanalyzer. The libraries were prepared according to Illumina instructions for RNA sample preparation: TruSeq RNA V2.

text: metamorphosis stage i

characteristic: developmental stage

text: Sycon ciliatum

text: Sycon ciliatum

text: Sycon ciliatum


text: Sars International Centre for Marine Molecular Biology

text: public

text: ArrayExpress

text: metamorphosis stage v

text: Sycon ciliatum

text: Sycon ciliatum

text: Sycon ciliatum


text: public

text: ArrayExpress

text: metamorphosis stage iii

text: Sycon ciliatum

text: Sycon ciliatum

text: Sycon ciliatum


text: public

text: ArrayExpress

text: metamorphosis stage ii

text: Sycon ciliatum

text: Sycon ciliatum

text: Sycon ciliatum


text: public

text: ArrayExpress

text: metamorphosis stage iv

text: Sycon ciliatum

text: Sycon ciliatum

text: Sycon ciliatum


text: public

text: ArrayExpress

text: metamorphosis stage i

text: Sycon ciliatum

text: Sycon ciliatum

text: Sycon ciliatum


text: public

text: ArrayExpress

text: young syconoid

text: Sycon ciliatum

text: Sycon ciliatum

text: Sycon ciliatum


text: public

tag: Namespace:HZI - HELMHOLTZ CENTRE FOR INFECTION RESEARCH, BRAUNSCHWEIG, GERMANY

text: house mouse

characteristic: common name

text: Analysis of the CD94 encoding gene Klrd1 revealed isogenic DBA/2 mice

text: Mus musculus

text: Mus musculus


characteristic: ENA-CHECKLIST

text: public

characteristic: collection date

text: Germany

characteristic: geographic location (country and/or sea)

text: Staphylococcus aureus

text: Staphylococcus aureus


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SLU-GBC

text: public

text: Staphylococcus aureus strain ILRI_Eymole1/1 has been isolated from nasal swab of camel from Kenya. It is a Gram positive infectious parasite of human, camel, cattle and other animals. It affects its hosts with infections like respiratory infections, skin infections and mastitis etc.

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (material)

characteristic: environmental package

characteristic: geographic location (altitude)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: investigation type

text: Staphylococcus aureus isolate ILRI_Eymole1/1

characteristic: isolate

text: Staphylococcus aureus

characteristic: project name

text: Staphylococcus aureus

characteristic: sequencing method

text: Complete genome sequence of Camelus dromedarius Staphylococcus aureus ILRI_Eymole1/1


characteristic: ENA-CHECKLIST

text: PERIO-UKM

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Israel

text: Legionella pneumophila

characteristic: organism

text: Legionella pneumophila

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Genoscreen

text: public

characteristic: broker name

characteristic: description

text: Campylobacter coli

text: Campylobacter coli


text: Genoscreen

text: public

text: Campylobacter jejuni

text: Campylobacter jejuni


text: Genoscreen

text: public

text: Campylobacter sp.

text: Campylobacter sp.


text: public

characteristic: collection date

text: Germany

characteristic: geographic location (country and/or sea)

text: Homo sapiens

characteristic: host

text: Yes

characteristic: host associated

text: Staphylococcus aureus

text: Staphylococcus aureus


characteristic: ENA-CHECKLIST

characteristic: INSDC center name

text: public

tag: Namespace:Max Planck Institute of Immunobiology and Epigenetics

text: ATCC CCL-186

characteristic: bio material

characteristic: cell line

text: fibroblast

characteristic: cell type

text: human

characteristic: common name

text: Homo sapiens

text: Homo sapiens

text: female

characteristic: sex

text: lung

characteristic: tissue type

text: IMR-90 human diploid fibroblast cell line


characteristic: ENA-FIRST-PUBLIC

text: GENEVA UNIVERSITY HO

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:GENEVA UNIVERSITY HO

characteristic: Submitter Id

text: human

text: Homo sapiens

characteristic: organism

text: Homo sapiens

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: URMITE

text: public

text: Draft genome sequence of Legionella massiliensis LegA

characteristic: description

text: Legionella massiliensis

text: Legionella massiliensis

text: LegA

characteristic: strain

text: Legionella massiliensis LegA, genome sequence


characteristic: ENA-CHECKLIST

text: DTU

characteristic: INSDC center name

text: public

text: Salmonella enterica subsp. enterica serovar Typhi

text: S.Typhi


text: Genoscope CEA

text: public

text: Xanthomonas citri pv. citri

text: Xanthomonas citri pv. citri

text: Procaryotic genome sequencing


text: Systems Biology Laboratory UK

text: public

tag: Namespace:Systems Biology Laboratory UK

text: house mouse

characteristic: common name

text: Mus musculus

text: Mus musculus


characteristic: Days of differentation

text: LEIDEN UNIVERSITY MEDICAL CENTER

text: public

tag: Namespace:LEIDEN UNIVERSITY MEDICAL CENTER

text: house mouse

text: Mus musculus

text: Mus musculus


Liver gDNA

text: Systems Biology Laboratory UK

text: public

text: Liver gDNA

text: house mouse

text: Mus musculus

text: Mus musculus

text: Liver gDNA


characteristic: Days of differenation

text: LEIDEN UNIVERSITY MEDICAL CENTER

text: public

text: harringtonin

characteristic: Treatment

text: house mouse

text: Mus musculus

text: Mus musculus


characteristic: ENA-FIRST-PUBLIC

text: LEIDEN UNIVERSITY MEDICAL CENTER

characteristic: INSDC first public

text: public

text: cycloheximide

text: house mouse

text: Mus musculus

text: Mus musculus


text: UCONNMISEQ

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Aeromonas veronii bv. sobria LMG 13067

text: Aeromonas veronii bv. sobria

text: Aeromonas veronii bv. sobria

text: LMG 13067

text: Aeromonas veronii bv. sobria LMG 13067


text: UCONNMISEQ

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: Aeromonas veronii CECT 4486

text: Aeromonas veronii

text: Aeromonas veronii

text: CECT 4486

text: Aeromonas veronii CECT 4486


text: UCONNMISEQ

text: public

text: Aeromonas hydrophila subsp. ranae CIP 107985

text: Aeromonas hydrophila subsp. ranae

characteristic: organism

text: Aeromonas hydrophila subsp. ranae

text: CIP 107985

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCONNMISEQ

text: public

text: Aeromonas dhakensis CIP 107500

text: Aeromonas dhakensis

text: Aeromonas dhakensis

text: CIP 107500

text: Aeromonas dhakensis CIP 107500


text: UCONNMISEQ

text: public

text: Aeromonas veronii CIP 107763

characteristic: description

text: Aeromonas veronii

text: Aeromonas veronii

text: CIP 107763

characteristic: strain

text: Aeromonas veronii CIP 107763


characteristic: ENA-CHECKLIST

text: UCONNMISEQ

characteristic: INSDC center name

text: public

text: Aeromonas caviae CECT 4221

text: Aeromonas caviae

text: Aeromonas caviae

text: CECT 4221

text: Aeromonas caviae CECT 4221


text: UCONNMISEQ

text: public

text: Aeromonas allosaccharophila BVH88

text: Aeromonas allosaccharophila

text: Aeromonas allosaccharophila

text: Aeromonas allosaccharophila BVH88


text: UCONNMISEQ

text: public

text: Aeromonas veronii bv. veronii CECT 4257

text: Aeromonas veronii bv. veronii

text: Aeromonas veronii bv. veronii

text: CECT 4257

text: Aeromonas veronii bv. veronii CECT 4257


text: UCONNMISEQ

text: public

text: Aeromonas enteropelogenes CECT 4255T

text: Aeromonas enteropelogenes

text: Aeromonas enteropelogenes

text: CECT 4255T

text: Aeromonas enteropelogenes CECT 4255T


text: UCONNMISEQ

text: public

text: Aeromonas tecta CECT 7082

text: Aeromonas tecta

text: Aeromonas tecta

text: CECT 7082

text: Aeromonas tecta CECT 7082


text: UCONNMISEQ

text: public

text: Aeromonas taiwanensis LMG 24683

text: Aeromonas taiwanensis

text: Aeromonas taiwanensis

text: LMG 24683

text: Aeromonas taiwanensis LMG 24683


text: UCONNMISEQ

text: public

text: Aeromonas sobria CECT 4245

text: Aeromonas sobria

text: Aeromonas sobria

text: CECT 4245

text: Aeromonas sobria CECT 4245


text: UCONNMISEQ

text: public

text: Aeromonas simiae CIP 107798

text: Aeromonas simiae

text: Aeromonas simiae

text: CIP 107798

text: Aeromonas simiae CIP 107798


text: UCONNMISEQ

text: public

text: Aeromonas schubertii CECT 4240

text: Aeromonas schubertii

text: Aeromonas schubertii

text: CECT 4240

text: Aeromonas schubertii CECT 4240


text: UCONNMISEQ

text: public

text: Aeromonas sanarellii LMG 24682

text: Aeromonas sanarellii

text: Aeromonas sanarellii

text: LMG 24682

text: Aeromonas sanarellii LMG 24682


text: UCONNMISEQ

text: public

text: Aeromonas salmonicida subsp. salmonicida CIP 103209

text: CIP 103209


text: UCONNMISEQ

text: public

text: Aeromonas rivuli DSM 22539

text: Aeromonas rivuli

text: Aeromonas rivuli

text: DSM 22539

text: Aeromonas rivuli DSM 22539


text: UCONNMISEQ

text: public

text: Aeromonas popoffii CIP 105493

text: Aeromonas popoffii

text: Aeromonas popoffii

text: CIP 105493

text: Aeromonas popoffii CIP 105493


text: UCONNMISEQ

text: public

text: Aeromonas piscicola LMG 24783

text: Aeromonas piscicola

text: Aeromonas piscicola

text: LMG 24783

text: Aeromonas piscicola LMG 24783


text: UCONNMISEQ

text: public

text: Aeromonas media CECT 4232

text: Aeromonas media

text: Aeromonas media

text: CECT 4232

text: Aeromonas media CECT 4232


text: UCONNMISEQ

text: public

text: Aeromonas jandaei CECT 4228

text: Aeromonas jandaei

text: Aeromonas jandaei

text: CECT 4228

text: Aeromonas jandaei CECT 4228


text: UCONNMISEQ

text: public

text: Aeromonas fluvialis LMG 24681

text: Aeromonas fluvialis

text: Aeromonas fluvialis

text: LMG 24681

text: Aeromonas fluvialis LMG 24681


text: UCONNMISEQ

text: public

text: Aeromonas eucrenophila CECT 4224

text: Aeromonas eucrenophila

text: Aeromonas eucrenophila

text: CECT 4224

text: Aeromonas eucrenophila CECT 4224


text: UCONNMISEQ

text: public

text: Aeromonas enteropelogenes CECT 4487

text: Aeromonas enteropelogenes

text: Aeromonas enteropelogenes

text: CECT 4487

text: Aeromonas enteropelogenes CECT 4487


text: UCONNMISEQ

text: public

text: Aeromonas encheleia CECT 4342

text: Aeromonas encheleia

text: Aeromonas encheleia

text: CECT 4342

text: Aeromonas encheleia CECT 4342


text: UCONNMISEQ

text: public

text: Aeromonas diversa CECT 4254

text: Aeromonas diversa

text: Aeromonas diversa

text: CECT 4254

text: Aeromonas diversa CECT 4254


text: UCONNMISEQ

text: public

text: Aeromonas caviae CECT 838

text: Aeromonas caviae

text: Aeromonas caviae

text: CECT 838

text: Aeromonas caviae CECT 838


text: UCONNMISEQ

text: public

text: Aeromonas bivalvium CECT 7113

text: Aeromonas bivalvium

text: Aeromonas bivalvium

text: CECT 7113

text: Aeromonas bivalvium CECT 7113


text: UCONNMISEQ

text: public

text: Aeromonas bestiarum CECT 4227

text: Aeromonas bestiarum

text: Aeromonas bestiarum

text: CECT 4227

text: Aeromonas bestiarum CECT 4227


text: UCONNMISEQ

text: public

text: Aeromonas australiensis CECT 8023

text: Aeromonas australiensis

text: Aeromonas australiensis

text: CECT 8023

text: Aeromonas australiensis CECT 8023


text: UCONNMISEQ

text: public

text: Aeromonas dhakensis CECT 7289

text: Aeromonas dhakensis

text: Aeromonas dhakensis

text: CECT 7289

text: Aeromonas dhakensis CECT 7289


text: UCONNMISEQ

text: public

text: Aeromonas allosaccharophila CECT 4199

text: Aeromonas allosaccharophila

text: Aeromonas allosaccharophila

text: CECT 4199

text: Aeromonas allosaccharophila CECT 4199


text: UCONNMISEQ

text: public

text: Aeromonas hydrophila AH4

text: Aeromonas hydrophila

text: Aeromonas hydrophila

text: Aeromonas hydrophila AH4


text: URMITE

text: public

text: Bacteria abnormis

text: Bacteria abnormis

text: Anoroxic


text: URMITE

text: public

text: Bacteria aborigena

text: Bacteria aborigena

text: Control


text: IOA

text: public

text: Scotland

text: Mytilus edulis

text: Mytilus edulis


text: Genoscope CEA

text: public

text: Xanthomonas citri pv. citri

text: Xanthomonas citri pv. citri

text: Procaryotic genome sequencing


text: URMITE

text: public

text: Bacteria acuminatocercata

text: Bacteria acuminatocercata

text: KWASHIORGUT


text: URMITE

text: public

text: Bacteria abnormis

text: Bacteria abnormis

text: Marasme


text: MDC

text: public

text: house mouse

characteristic: common name

text: Mus musculus

text: Mus musculus


text: URMITE

text: public

text: Bacteria abnormis

text: Bacteria abnormis

text: AnoroxicS


text: URMITE

text: public

text: Bacteria abnormis

text: Bacteria abnormis

text: ControlS


text: URMITE

text: public

text: Bacteria abnormis

text: Bacteria abnormis

text: KWASHIORGUTS


text: URMITE

text: public

text: Bacteria abnormis

text: Bacteria abnormis

text: MarasmeS


text: CHINESE ACADEMY OF FISHERY SCIENCE

text: public

tag: Namespace:CHINESE ACADEMY OF FISHERY SCIENCE

text: common carp

text: Cyprinus carpio

text: Cyprinus carpio

text: common carp


text: MRC CLINICAL SCIENCES CENTRE, IMPERIAL COLLEGE LONDON, DU CANE ROAD, LONDON W12 0NN, UK

text: public

text: This strain is derived from the Long Term Evolution Experiment initiated by Richard Lenski.

text: Escherichia coli B str. REL607

text: Escherichia coli B str. REL607

text: E. coli B

text: E. coli B REL607

characteristic: sub strain

text: REL606-derived clone sampled after ~20k generations of laboratory evolution


text: public

characteristic: collection date

text: Germany

characteristic: geographic location (country and/or sea)

text: Staphylococcus aureus

text: Staphylococcus aureus


characteristic: ENA-FIRST-PUBLIC

text: WARWICK

characteristic: INSDC first public

text: public

text: Metagenomics of TB-associated sputum

text: anthropogenic terrestrial biome (ENVO:01000219)

characteristic: environment (biome)

text: lung (UBERON:0002048); infection (EFO:0000544); bacterial disease (EFO:0000771)

characteristic: environment (feature)

text: sputum (UBERON:0007311)

characteristic: environment (material)

text: Gambia

text: Homo sapiens

characteristic: host scientific name

characteristic: host taxid

text: clinical metagenome

characteristic: pubmed id

text: clinical metagenome

text: TB Sputum metagenome


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Staphylococcus aureus

characteristic: organism

text: Staphylococcus aureus

characteristic: title


characteristic: ENA-CHECKLIST

text: IOA

characteristic: INSDC center name

text: public

text: Z.Trumbic

characteristic: collected by

characteristic: collection date

text: Atlantic bluefin guna

characteristic: common name

text: Pool of 3 individuals (male and female, immature), different tissues (gill, gonad, heart, intestine, head kidney, liver, spleen, whole blood, red and white muscle, skin scrape)

characteristic: description

text: mix

characteristic: dev stage

text: Croatia, Adriatic sea, Island of Ugljan

characteristic: geographic location (country and/or sea)

text: Thunnus thynnus

text: Thunnus thynnus

text: mix

characteristic: sex

text: RNAlater at -20°C

text: pool

characteristic: tissue type


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: IOA

characteristic: INSDC first public

text: public

text: Atlantic cod

text: Pool of 40 multiplexed samples (barcoded).

text: Faroe

text: Gadus morhua

text: Gadus morhua

text: mix

text: fin clip


text: TSL

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Madagascar periwinkle

text: A BAC with the locus of the gene T16H

text: Catharanthus roseus

text: Catharanthus roseus

text: C. roseus BAC T16H


characteristic: ENA-CHECKLIST

text: TSL

characteristic: INSDC center name

text: public

text: Madagascar periwinkle

text: A BAC with the locus of the gene SGD

text: Catharanthus roseus

characteristic: organism

text: Catharanthus roseus

text: C. roseus BAC SGD

characteristic: title


text: URMITE

text: public

text: Lactococcus garvieae

text: Lactococcus garvieae

characteristic: strain

text: Lactococcus garvieae


E-MTAB-2929:smeg RNA-seq rep1

text: public

text: E-MTAB-2929:smeg RNA-seq rep1

tag: Namespace:Wadsworth Center, New York State of Health

text: ArrayExpress

characteristic: broker name

text: Protocols: 200 ml 7H9 medium was inoculated with 2.0 ml of overnight MC2 155 culture, and grew at 37 degrees C to an OD600 of ~1.0. The cells were harvested by pretreatment with chloramphenicol to a final concentration of 100 ug/ml for 2 minutes followed by rapid filtration by using a 500ml 0.45um PES filter system (Celltreat). Cells were collected and flash frozen in liquid nitrogen together with 0.7 ml lysis buffer (Oh et al. 2011). The frozen cells were pulverized 6 times at 15 Hz for 3 min by a mixer mill (Retsch MM400). The grinding jars were re-chilled in liquid nitrogen to keep the cells frozen between each cycle. After the pulverized cells were recovered, small scoopful was saved for bacterial transcript enrichment. The pulverized cell powder was extracted with acid phenol and chloroform followed by isopropanol precipitation. 16S and 23S ribosomal RNAs were removed by subtractive hybridization using an Epicentre Ribo-Zero Magnetic kit following manufacturer's manual. The enriched mRNAs were randomly fragmented as described (Oh et al. 2011) The RNA molecules were dephosphorylated by treating with T4 polynucleotide kinase (New England Biolabs). Then polyacrylamide gel purification was performed for size selection of ~28nt RNA fragments. Approximately 25-30 nt poly-A tails were added to recovered RNA fragments with an Ambion poly(A) tailing kit (Life Technologies) following the manufacturer's guide. The polyadenylated RNA samples were reverse transcribed using JW2364 and SuperScript III (Life Technologies), and the reverse transcription products were circularized by CircLigaseTM ssDNA ligase (Epicentre). PCR amplification was performed by using the circularized cDNA as template, JW2365 and JW2366 (Table 1) as primers, and Phusion High-Fidelity DNA Polymerase (New England Biolabs).

characteristic: description

text: Mycobacterium smegmatis str. MC2 155

text: Mycobacterium smegmatis str. MC2 155

text: Mycobacterium smegmatis str. MC2 155

text: smeg RNA-seq rep1


E-MTAB-2929:smeg Ribo-seq rep1

text: Wadsworth Center, New York State of Health

text: public

text: E-MTAB-2929:smeg Ribo-seq rep1

text: ArrayExpress

text: Protocols: 200 ml 7H9 medium was inoculated with 2.0 ml of overnight MC2 155 culture, and grew at 37 degrees C to an OD600 of ~1.0. The cells were harvested by pretreatment with chloramphenicol to a final concentration of 100 ug/ml for 2 minutes followed by rapid filtration by using a 500ml 0.45um PES filter system (Celltreat). Cells were collected and flash frozen in liquid nitrogen together with 0.7 ml lysis buffer (Oh et al. 2011). The frozen cells were pulverized 6 times at 15 Hz for 3 min by a mixer mill (Retsch MM400). The grinding jars were re-chilled in liquid nitrogen to keep the cells frozen between each cycle. After the pulverized cells were recovered, small scoopful was saved for bacterial transcript enrichment. The pulverized cells were thawed. The clarified lysates were treated with micrococcal nuclease (MNase, Worthington Biochemical Corp). Monosomes were isolated by sucrose gradient fractionation. mRNA footprints were purified with acid phenol and chloroform extraction, and isopropanol precipitation. The RNA molecules were dephosphorylated by treating with T4 polynucleotide kinase (New England Biolabs). Then polyacrylamide gel purification was performed for size selection of ~28nt RNA fragments. Approximately 25-30 nt poly-A tails were added to recovered RNA fragments with an Ambion poly(A) tailing kit (Life Technologies) following the manufacturer's guide. The polyadenylated RNA samples were reverse transcribed using JW2364 and SuperScript III (Life Technologies), and the reverse transcription products were circularized by CircLigaseTM ssDNA ligase (Epicentre). PCR amplification was performed by using the circularized cDNA as template, JW2365 and JW2366 (Table 1) as primers, and Phusion High-Fidelity DNA Polymerase (New England Biolabs).

text: Mycobacterium smegmatis str. MC2 155

text: Mycobacterium smegmatis str. MC2 155

text: Mycobacterium smegmatis str. MC2 155

text: smeg Ribo-seq rep1


E-MTAB-2929:smeg RNA-seq rep2

text: public

text: E-MTAB-2929:smeg RNA-seq rep2

text: ArrayExpress

text: Mycobacterium smegmatis str. MC2 155

text: Mycobacterium smegmatis str. MC2 155

text: Mycobacterium smegmatis str. MC2 155

text: smeg RNA-seq rep2


E-MTAB-2929:smeg Ribo-seq rep2

text: public

text: E-MTAB-2929:smeg Ribo-seq rep2

text: ArrayExpress

text: Mycobacterium smegmatis str. MC2 155

text: Mycobacterium smegmatis str. MC2 155

text: Mycobacterium smegmatis str. MC2 155

text: smeg Ribo-seq rep2


text: UFMG

text: public

characteristic: bio material

characteristic: collection date

text: Leishmania peruviana LEM-1537

text: Peru

characteristic: geographic location (country and/or sea)

text: Homo sapiens

characteristic: host

text: Leishmania peruviana

text: Leishmania peruviana


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UFMG

text: public

text: Leishmania peruviana PAB-4377

text: Peru

text: Homo sapiens

text: Leishmania peruviana

text: Leishmania peruviana


text: PUBLIC HEALTH ENGLAND

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

tag: Namespace:PUBLIC HEALTH ENGLAND

text: United Kingdom

text: Peptoclostridium difficile

text: Peptoclostridium difficile

text: Clostridium difficile strains


tag: attribute

characteristic: ENA-CHECKLIST

text: HZI - HELMHOLTZ CENTRE FOR INFECTION RESEARCH, BRAUNSCHWEIG, GERMANY

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: Yersinia pseudotuberculosis YPIII

text: Yersinia pseudotuberculosis YPIII

text: YPIII RNA-Seq


Borrelia crocidurae str. 03-02

text: URMITE

characteristic: INSDC center name

text: public

text: Borrelia crocidurae str. 03-02

text: Borrelia crocidurae

characteristic: organism

text: Borrelia crocidurae

text: Borrelia crocidurae genome

characteristic: title


Paired End BACs human

text: IPK-Gatersleben

text: public

text: Paired End BACs human

text: human

characteristic: common name

text: dilution series of two human BACs (AF252830, AF285443) for test reasons

characteristic: description

text: Homo sapiens

text: Homo sapiens

text: Paired End BACs human


text: public

tag: Namespace:Department of Plant Sciences University of Cambridge

text: ArrayExpress

characteristic: broker name

text: Protocols: Chlamydomonas reinhardtii cells (CC-4350 cw Arg 7-8 mt+) (Chlamydomonas Resource Center [http://chlamycollection.org/strains/]) were maintained in 750 ml Tris-acetate-phosphate medium (Harris, 1989) at 23 C on a rotatory shaker (140 rpm) under constant illumination with white light (70 uE.m2.sec-1) to mid-log phase (OD750 ~ 0.6). Chlamydomonas cultures were harvested by filtering off the media, the cell paste was flash frozen and pulverized in liquid nitrogen with 5 ml of pre-frozen lysis buffer (20 mM Tris-Cl pH7.5, 140 mM KCl, 5 mM MgCl2, 100 ug/ml cycloheximide, 100 ug/ml chloramphenicol, 0.05 mM DTT, 0.1% NP40 and 5% sucrose). The frozen powder was thawed on ice and clarified by centrifugation for 30 min at 4700 rpm at 4 C followed by adjustment of A254 to ~4 before snap-freezing in liquid nitrogen and storage at -80 C. For Chlamydomonas, lysates were slowly thawed on ice and a 200 ul aliquot (A254 = 4) treated with 300 U RNAse 1 (100 U/ul, Life Technologies cat. no. AM2294) in a thermo-mixer at 28 C, 400 rpm for 30 min. The tube was placed on ice, 2 ul of SUPERase-In RNase inhibitor (20 U/ml, Life Technologies) added and the reaction layered onto a 1 M sucrose cushion prepared in Chlamydomonas polysome buffer (20 mM Tris-HCl pH 7.5, 140 mM KCl, 5 mM MgCl2, 0.5 mM DTT, 100 ug/ml cycloheximide, 100 ug/ml chloramphenicol and 0.5 ug/ml SUPERase-In.). The cushion was ultracentrifuged at 38,000 rpm (5 hr, 4C) in a Beckman Sw41Ti rotor. 150 ul of each cell lysate was digested with proteinase K and cellular RNA extracted using acidic phenol:chloroform as above. Ribosomal RNA was depleted from 5 ug of total RNA using a Ribo-Zero rRNA removal kit targeting the appropriate species following the manufacturers instructions (Human/Mouse/Rat: Epicentre, cat. no. RZH1046, Plant Seed/root: Epicentre, Cat. No. MRZSR116). Depleted RNA was resuspended in 10 mM Tris-HCl pH 7.5 and quantified by spectrophotometry. 1-2 ug of total RNA in 20 ul was mixed with an equal volume of 2X alkaline fragmentation solution (2 mM EDTA, 10 mM Na2CO3 and 90 mM NaHCO3) and incubated for 15 min at 95 C. The reaction was diluted by addition of 280 ul stop/precipitation solution (300 mM NaOAc pH 5.5, GlycoBlue Co-precipitant [Ambion, 15 mg/ml]), and fragmented RNA recovered by ethanol precipitation. Fragmented total RNA or RPFs (1-2 ug) were separated on 15% (wt/vol) denaturing polyacrylamide gels and RNA species migrating between 28 and 34 nt harvested. RNA was eluted from the gel slices on a rotator overnight at 4 C, in 600 ul RNA gel extraction buffer (300 mM NaOAc pH 5.5, 1 mM EDTA and 0.25% SDS). Eluted RNA was ethanol precipitated. The RNA samples from above were heated at 80 C for 2 min, cooled and the 3' phosphate group removed using T4 polynucleotide kinase (T4 PNK, New England Biolabs) at 37 C for 2 hrs in a 20 ul reaction lacking ATP. The RNA was concentrated by ethanol precipitation, resuspended in 10 mM Tris-HCl pH 7.5 and ligated in a 20 ul reaction overnight at 14 C to a preadenylated 3'-adaptor (5' rATGGAATTCTCGGGTGCCAAGG 3') using T4 RNA Ligase 2 truncated K227Q (New England Biolabs). This 3' adaptor was adenylated using a 5' DNA adenylation kit (New England Biolabs) following the manufacturers instructions. RNA was precipitated, loaded into a 15% (wt/vol) denaturing polyacrylamide gel and ligated RNA fragments migrating between 49 nt and 53 nt excised. The RNA was eluted, precipitated, resuspended as above and 5' phosphorylated using T4 PNK in the presence of 1mM ATP for 2 hrs at 37 C. RNA was concentrated by ethanol precipitation, resuspended in 10mM Tris-HCl pH 7.5 and ligated to a 5' RNA adaptor (5' rGrUrUrCrArGrArGrUrUrCrUrArCrArGrUrCrCrGrArCrGrArUrC 3') in a 20 ul reaction overnight at 14 C using T4 RNA Ligase (Promega). The fully adapted RNAs were recovered by ethanol precipitation, dissolved in 6 ul 10mM Tris-HCl pH 7.5 and 3 ul of this ligated product annealed to an RT primer (5' GCCTTGGCACCCGAGAATTCCA, 50 pmol) at 65 C for 5 min. The RNA was subsequently reverse transcribed at 55 C for 50 min in a 20 ul reaction following addition of first strand buffer (Invitrogen, to 1X), 2.5 mM MgCl2, 10 mM DTT, 0.5 ul SUPERase-In and 1 ul SuperScript III (Invitrogen), followed by heat inactivation at 85C for 5 min. For PCR and ribosomal depletion steps, see referenced manuscript.

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii


text: Department of Plant Sciences University of Cambridge

text: public

text: ArrayExpress

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii

text: Chlamydomonas reinhardtii


text: public

text: YPIII RNA-Seq

text: Yersinia pseudotuberculosis YPIII

text: Yersinia pseudotuberculosis YPIII

text: YPIII RNA-Seq


text: AAU, India

text: public

characteristic: Is the sequenced pathogen host associated?

tag: Namespace:AAU, India

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host health state

characteristic: host scientific name

characteristic: isolation source host-associated

characteristic: isolation source non-host-associated

text: Bos indicus

text: Bos indicus

text: workshop


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Guangzhou iGenomics Co.,Ltd

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

tag: Namespace:Guangzhou iGenomics Co.,Ltd

text: ArrayExpress

text: Protocols: The liver fluke flukes were collected from cats livers, and the four tissues (oral sucker, muscle, testis and ovary) were isolated form the flukes for RNA extraction. RNA extraction from the liver fluke organs by Trizol. The sequencing library was constructed as described in the TruSeq RNA SamplePrep Guide (Illumina). The fragments were enriched by PCR for 15 cycles and excised at 400 bp on a 2% agarose gel as the final libraries.

text: Clonorchis sinensis

text: Clonorchis sinensis

text: muscle

characteristic: organism part

text: Clonorchis sinensis

text: He Nan, China

characteristic: strain

text: muscle


text: Guangzhou iGenomics Co.,Ltd

characteristic: INSDC center name

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

characteristic: description

text: Clonorchis sinensis

characteristic: organism

text: Clonorchis sinensis

text: testis

text: Clonorchis sinensis

text: He Nan, China

text: testis

characteristic: title


text: Guangzhou iGenomics Co.,Ltd

text: public

text: ArrayExpress

text: Clonorchis sinensis

text: Clonorchis sinensis

text: oral sucker

text: Clonorchis sinensis

text: He Nan, China

text: sucker


text: Guangzhou iGenomics Co.,Ltd

text: public

text: ArrayExpress

text: Clonorchis sinensis

text: Clonorchis sinensis

text: ovary

text: Clonorchis sinensis

text: He Nan, China

text: ovary


text: HSL-SP

text: public

text: house mouse

characteristic: common name

text: Mus musculus

text: Mus musculus


characteristic: ENA-CHECKLIST

text: public

text: YPIII RNA-Seq

text: Yersinia pseudotuberculosis YPIII

text: Yersinia pseudotuberculosis YPIII

text: YPIII


Rickettsia hoogstraalii Croatica

characteristic: ENA-FIRST-PUBLIC

text: URMITE

text: public

text: Rickettsia hoogstraalii Croatica

text: Rickettsia hoogstraalii

text: Rickettsia hoogstraalii

text: Croatica

text: Rickettsia hoogstraalii Croatica genome


characteristic: ENA-LAST-UPDATE

text: URMITE

text: public

text: Afipia felis genospecies A

text: Afipia felis genospecies A

text: afipia


characteristic: External Id

text: WUR

text: public

characteristic: culture collection

text: Romboutsia lituseburensis

text: Romboutsia lituseburensis

text: Genome sequence of Romboutsia lituseburensis


text: WUR

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: Romboutsia lituseburensis

text: Romboutsia lituseburensis

text: Genome sequence of Romboutsia lituseburensis - MiSeq


text: PNRI

text: public

characteristic: INSDC status

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: public

tag: Namespace:LUXEMBOURG CENTRE FOR SYSTEMS BIOMEDICINE

text: human

text: SH-SY5Y cell culture SH-SY5Y cells were cultured from a passage 23 (P23) vial, purchased directly from ATCC (CRL-2266) (Figure 3). In the first step the cells were amplified during 4 passages for preparing passage 27 (P27) freezing stocks. The cells were furthermore amplified during 5 passages for preparing passage 33 (P33) samples for omics analysis. DNA was extracted with the High Pure PCR Template Preparation Kit (Cat. #11796828001, Roche). The samples sequenced by Complete Genomics and Illumina platforms were of equal passage and derived from the same frozen cell stock. For RNA analysis the cells were lysed in Qiazol buffer and were frozen at -80 °C. RNA extraction was performed using the miRNeasy Kit (Qiagen). For protein analysis the cells were washed two times with DPBS and for inhibition of proteases the cells were covered with complete (Roche) solution in ddH2O and were frozen at -80 °C. RNA Extraction P33 cells were detached using Trypsin (Cat. #25300, Gibco) and after centrifugation, the cell pellet was lysed in QIAzol buffer and frozen at -80 °C. The cell pellet in QIAzol was thawed. Chloroform was added and the sample was centrifuged at 12000 g for 15 min at 4 °C. The upper aqueous layer was removed and 1.5 times the volume of the aqueous layer of ethanol was added. RNA in the aqueous layer was bound to an RNeasy Mini column by loading the aqueous layer + ethanol to the top of the column and spinning at 8000 g for 15 s at room temperature. The column was washed once with Buffer RWT, then twice with Buffer RPE. RNA was eluted from the column with 2 washes of 30 μL of water. Strand-specific RNA-Seq library preparation PolyA RNA was isolated from 500 ng of input RNA using oligo(dT)25 Dynabeads (Invitrogen) by binding the RNA to the Dynabeads, collecting the beads with a magnetic rack, and removing the supernatant containing non-polyA RNA. The Dynabeads were washed in washing buffer then the mRNA was eluted in TE buffer. The binding and washing steps were repeated to remove remaining non-polyA RNA. After the binding and washing, the Dynabeads were resuspended in 2X Superscript III first-strand buffer with 10 mM DTT and incubated at 94 °C for 6 min to fragment the mRNA then immediately cooled on ice. The Dynabeads were collected on a magnetic stand and the fragment mRNA was moved to a new tube. Fragmented mRNA in 2x RT buffer, random hexamers, and RNasin Plus was heated at 50 °C for 1 min to denature the RNA then placed immediately on ice. To this, water, Actinomycin D, DTT, dNTPs, and SuperScript III (Invitrogen) were added and reverse transcription was performed by incubating the mixture at 25 °C for 10 min then at 50 °C for 50 min. RNAClean XP beads (Agencourt) were added to the mixture and incubated on ice for 15 min. The beads were collected on a magnetic stand, washed twice with 75 % ethanol then air-dried for 5 min. The RNA/cDNA hybrid was eluted in water. A second strand reaction master mix including either 10x Blue Buffer or NEBuffer 2, dNTP (with dATP, dCTP, dGTP, and dUTP), RNase H, DNA polymerase I (Enzymatics), and water was added to each sample and incubated at 16 °C for 2.5 h. Double-stranded DNA was purified using 1.8 volumes of AMPure XP beads (Agencourt) and eluted in water. The DNA was dA-tailed by adding either 10x Blue Buffer or NEBuffer 2, dATP, water, and Klenow 3’-5’ exo (Enzymatics) and incubated at 37 °C for 30 min. The dsDNA was purified using AMPure XP beads, and eluted in water. To each sample, a specific barcode adapter was added, along with Rapid Ligation Buffer and T4 DNA Ligase HC (Enzymatics). The Y-shape adapter ligation was performed by incubating at room temperature for 15 min. DNA was purified and size selected by cleaning up with AMPure XP beads 3 consecutive times and eluting in TE buffer. The first cleanup was performed with 1 volume of AMPure XP beads, the second with 1.4 volumes, and the last with 1 volume incubated at room temperature for 5 min without washing to perform size selection. The supernatant was transferred to a tube with half of the new volume of AMPure XP beads and incubated at room temperature for 15 min. The beads were collected on a magnetic stand and washed twice with 75 % ethanol. The DNA was eluted in water. The second strand of DNA was digested with Uracil DNA Glycosylase (Enzymatics) at 37 °C for 15 min. The PCR reaction was set up containing UDG-digested DNA, primer A and B, Phusion HF Buffer, dNTP, water, and Phusion II (New England Biolabs). The reaction was incubated at 94 °C for 2 min, followed by 10-12 cycles of amplification (98 °C for 10 s, 65 °C for 30 s, 72 °C for 30 s). The DNA libraries were purified with 1.4 volumes of AMPure XP beads and eluted in TE buffer.

text: Homo sapiens

text: Homo sapiens


Coxiella burnetii Cb196_Saudi_Arabia

text: URMITE

text: public

text: Coxiella burnetii Cb196_Saudi_Arabia

text: Coxiella burnetii CbuK_Q154

text: Coxiella burnetii CbuK_Q154

characteristic: strain

text: Coxiella burnetii Cb196 from Saudi Arabia


A1A (bacteria; compost)

text: JIC

characteristic: INSDC center name

text: public

text: A1A (bacteria; compost)

text: abandoned grassland

characteristic: current land use

text: grass

characteristic: current vegetation

text: 0.1-0.3 m

characteristic: depth

text: Arabidopsis 1st gen

text: UK; Norfolk; Bawburgh; JIC Church Farm

characteristic: geographic location (country and/or sea, region)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: compost

text: 33.55 mg/kg

characteristic: magnesium

text: 3.49 mg/kg

characteristic: nitrate

text: doi: 10.1128/AEM.66.12.5488-5491.2000

characteristic: nucleic acid extraction

characteristic: organic matter

text: soil metagenome

characteristic: pcr conditions

text: 120.5 mg/kg

characteristic: phosphate

text: 168.2 mg/kg

characteristic: potassium

text: soil metagenome

text: Luvisol WRB

characteristic: soil type

text: 16S rDNA

characteristic: target gene

characteristic: target subfragment

text: loamy

characteristic: texture

text: A1A (bacteria)


A1B (bacteria; compost)

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: JIC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: A1B (bacteria; compost)

characteristic: Submitter Id

text: abandoned grassland

characteristic: current land use

text: grass

text: 0.1-0.3 m

text: Arabidopsis 1st gen

characteristic: description

text: UK; Norfolk; Bawburgh; JIC Church Farm

text: compost

text: 33.55 mg/kg

text: 3.49 mg/kg

text: doi: 10.1128/AEM.66.12.5488-5491.2000

text: soil metagenome

characteristic: organism

text: 120.5 mg/kg

text: 168.2 mg/kg

text: soil metagenome

text: Luvisol WRB

text: 16S rDNA

text: loamy

text: A1B (bacteria)

characteristic: title


A1C (bacteria; compost)

text: JIC

text: public

text: A1C (bacteria; compost)

text: abandoned grassland

text: grass

characteristic: current vegetation

text: 0.1-0.3 m

characteristic: depth

text: Arabidopsis 1st gen

text: UK; Norfolk; Bawburgh; JIC Church Farm

characteristic: geographic location (country and/or sea, region)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: compost

text: 33.55 mg/kg

characteristic: magnesium

text: 3.49 mg/kg

characteristic: nitrate

text: doi: 10.1128/AEM.66.12.5488-5491.2000

characteristic: nucleic acid extraction

characteristic: organic matter

text: soil metagenome

characteristic: pcr conditions

text: 120.5 mg/kg

characteristic: phosphate

text: 168.2 mg/kg

characteristic: potassium

text: soil metagenome

text: Luvisol WRB

characteristic: soil type

text: 16S rDNA

characteristic: target gene

text: loamy

text: A1C (bacteria)


A2A (bacteria; compost)

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: JIC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: A2A (bacteria; compost)

characteristic: Submitter Id

text: abandoned grassland

characteristic: current land use

text: grass

text: 0.1-0.3 m

text: Arabidopsis 2nd gen

characteristic: description

text: UK; Norfolk; Bawburgh; JIC Church Farm

text: compost

text: 33.55 mg/kg

text: 3.49 mg/kg

text: doi: 10.1128/AEM.66.12.5488-5491.2000

text: soil metagenome

text: 120.5 mg/kg

text: 168.2 mg/kg

text: soil metagenome

text: Luvisol WRB

text: 16S rDNA

characteristic: target subfragment

text: loamy

characteristic: texture

text: A2A (bacteria)


A2B (bacteria; compost)

text: JIC

text: public

text: A2B (bacteria; compost)

text: abandoned grassland

text: grass

characteristic: current vegetation

text: 0.1-0.3 m

characteristic: depth

text: Arabidopsis 2nd gen

text: UK; Norfolk; Bawburgh; JIC Church Farm

characteristic: geographic location (country and/or sea, region)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: compost

text: 33.55 mg/kg

characteristic: magnesium

text: 3.49 mg/kg

characteristic: nitrate

text: doi: 10.1128/AEM.66.12.5488-5491.2000

characteristic: nucleic acid extraction

characteristic: organic matter

text: soil metagenome

characteristic: organism

characteristic: pcr conditions

text: 120.5 mg/kg

characteristic: phosphate

text: 168.2 mg/kg

characteristic: potassium

text: soil metagenome

text: Luvisol WRB

text: 16S rDNA

text: loamy

text: A2B (bacteria)

characteristic: title


A2C (bacteria; compost)

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: JIC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: A2C (bacteria; compost)

characteristic: Submitter Id

text: abandoned grassland

characteristic: current land use

text: grass

text: 0.1-0.3 m

text: Arabidopsis 2nd gen

characteristic: description

text: UK; Norfolk; Bawburgh; JIC Church Farm

text: compost

text: 33.55 mg/kg

text: 3.49 mg/kg

text: doi: 10.1128/AEM.66.12.5488-5491.2000

text: soil metagenome

text: 120.5 mg/kg

text: 168.2 mg/kg

text: soil metagenome

text: Luvisol WRB

characteristic: soil type

text: 16S rDNA

characteristic: target gene

text: loamy

text: A2C (bacteria)


A3A (bacteria; compost)

text: JIC

text: public

text: A3A (bacteria; compost)

text: abandoned grassland

text: grass

characteristic: current vegetation

text: 0.1-0.3 m

characteristic: depth

text: Arabidopsis 3rd gen

text: UK; Norfolk; Bawburgh; JIC Church Farm

characteristic: geographic location (country and/or sea, region)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: compost

text: 33.55 mg/kg

characteristic: magnesium

text: 3.49 mg/kg

characteristic: nitrate

text: doi: 10.1128/AEM.66.12.5488-5491.2000

characteristic: nucleic acid extraction

characteristic: organic matter

text: soil metagenome

characteristic: organism

characteristic: pcr conditions

text: 120.5 mg/kg

characteristic: phosphate

text: 168.2 mg/kg

text: soil metagenome

text: Luvisol WRB

text: 16S rDNA

characteristic: target subfragment

text: loamy

characteristic: texture

text: A3A (bacteria)


A3B (bacteria; compost)

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: JIC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: A3B (bacteria; compost)

characteristic: Submitter Id

text: abandoned grassland

characteristic: current land use

text: grass

text: 0.1-0.3 m

text: Arabidopsis 3rd gen

characteristic: description

text: UK; Norfolk; Bawburgh; JIC Church Farm

text: compost

text: 33.55 mg/kg

text: 3.49 mg/kg

text: doi: 10.1128/AEM.66.12.5488-5491.2000

text: soil metagenome

text: 120.5 mg/kg

text: 168.2 mg/kg

characteristic: potassium

text: soil metagenome

text: Luvisol WRB

text: 16S rDNA

text: loamy

text: A3B (bacteria)

characteristic: title


A3C (bacteria; compost)

text: JIC

text: public

text: A3C (bacteria; compost)

text: abandoned grassland

text: grass

characteristic: current vegetation

text: 0.1-0.3 m

characteristic: depth

text: Arabidopsis 3rd gen

text: UK; Norfolk; Bawburgh; JIC Church Farm

characteristic: geographic location (country and/or sea, region)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: compost

text: 33.55 mg/kg

characteristic: magnesium

text: 3.49 mg/kg

characteristic: nitrate

text: doi: 10.1128/AEM.66.12.5488-5491.2000

characteristic: nucleic acid extraction

characteristic: organic matter

text: soil metagenome

characteristic: organism

characteristic: pcr conditions

text: 120.5 mg/kg

characteristic: phosphate

text: 168.2 mg/kg

text: soil metagenome

text: Luvisol WRB

characteristic: soil type

text: 16S rDNA

characteristic: target gene

text: loamy

text: A3C (bacteria)


B1A (bacteria; compost)

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: JIC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: B1A (bacteria; compost)

characteristic: Submitter Id

text: abandoned grassland

characteristic: current land use

text: grass

text: 0.1-0.3 m

text: Brachypodium 1st gen

characteristic: description

text: UK; Norfolk; Bawburgh; JIC Church Farm

text: compost

text: 33.55 mg/kg

text: 3.49 mg/kg

text: doi: 10.1128/AEM.66.12.5488-5491.2000

text: soil metagenome

text: 120.5 mg/kg

text: 168.2 mg/kg

text: soil metagenome

text: Luvisol WRB

text: 16S rDNA

characteristic: target subfragment

text: loamy

characteristic: texture

text: B1A (bacteria)


B1B (bacteria; compost)

text: JIC

text: public

text: B1B (bacteria; compost)

text: abandoned grassland

text: grass

characteristic: current vegetation

text: 0.1-0.3 m

characteristic: depth

text: Brachypodium 1st gen

text: UK; Norfolk; Bawburgh; JIC Church Farm

characteristic: geographic location (country and/or sea, region)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: compost

text: 33.55 mg/kg

characteristic: magnesium

text: 3.49 mg/kg

characteristic: nitrate

text: doi: 10.1128/AEM.66.12.5488-5491.2000

characteristic: nucleic acid extraction

characteristic: organic matter

text: soil metagenome

characteristic: organism

characteristic: pcr conditions

text: 120.5 mg/kg

characteristic: phosphate

text: 168.2 mg/kg

characteristic: potassium

text: soil metagenome

text: Luvisol WRB

text: 16S rDNA

text: loamy

text: B1B (bacteria)

characteristic: title


B1C (bacteria; compost)

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: JIC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: B1C (bacteria; compost)

characteristic: Submitter Id

text: abandoned grassland

characteristic: current land use

text: grass

text: 0.1-0.3 m

text: Brachypodium 1st gen

characteristic: description

text: UK; Norfolk; Bawburgh; JIC Church Farm

text: compost

text: 33.55 mg/kg

text: 3.49 mg/kg

text: doi: 10.1128/AEM.66.12.5488-5491.2000

text: soil metagenome

text: 120.5 mg/kg

text: 168.2 mg/kg

text: soil metagenome

text: Luvisol WRB

characteristic: soil type

text: 16S rDNA

characteristic: target gene

text: loamy

text: B1C (bacteria)


B2A (bacteria; compost)

text: JIC

text: public

text: B2A (bacteria; compost)

text: abandoned grassland

text: grass

characteristic: current vegetation

text: 0.1-0.3 m

characteristic: depth

text: Brachypodium 2nd gen

text: UK; Norfolk; Bawburgh; JIC Church Farm

characteristic: geographic location (country and/or sea, region)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: compost

text: 33.55 mg/kg

characteristic: magnesium

text: 3.49 mg/kg

characteristic: nitrate

text: doi: 10.1128/AEM.66.12.5488-5491.2000

characteristic: nucleic acid extraction

characteristic: organic matter

text: soil metagenome

characteristic: organism

characteristic: pcr conditions

text: 120.5 mg/kg

characteristic: phosphate

text: 168.2 mg/kg

text: soil metagenome

text: Luvisol WRB

text: 16S rDNA

characteristic: target subfragment

text: loamy

characteristic: texture

text: B2A (bacteria)


B2B (bacteria; compost)

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: JIC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: B2B (bacteria; compost)

characteristic: Submitter Id

text: abandoned grassland

characteristic: current land use

text: grass

text: 0.1-0.3 m

text: Brachypodium 2nd gen

characteristic: description

text: UK; Norfolk; Bawburgh; JIC Church Farm

text: compost

text: 33.55 mg/kg

text: 3.49 mg/kg

text: doi: 10.1128/AEM.66.12.5488-5491.2000

text: soil metagenome

text: 120.5 mg/kg

text: 168.2 mg/kg

characteristic: potassium

text: soil metagenome

text: Luvisol WRB

text: 16S rDNA

text: loamy

text: B2B (bacteria)

characteristic: title


B2C (bacteria; compost)

text: JIC

text: public

text: B2C (bacteria; compost)

text: abandoned grassland

text: grass

characteristic: current vegetation

text: 0.1-0.3 m

characteristic: depth

text: Brachypodium 2nd gen

text: UK; Norfolk; Bawburgh; JIC Church Farm

characteristic: geographic location (country and/or sea, region)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: compost

text: 33.55 mg/kg

characteristic: magnesium

text: 3.49 mg/kg

characteristic: nitrate

text: doi: 10.1128/AEM.66.12.5488-5491.2000

characteristic: nucleic acid extraction

characteristic: organic matter

text: soil metagenome

characteristic: organism

characteristic: pcr conditions

text: 120.5 mg/kg

characteristic: phosphate

text: 168.2 mg/kg

text: soil metagenome

text: Luvisol WRB

characteristic: soil type

text: 16S rDNA

characteristic: target gene

text: loamy

text: B2C (bacteria)


B3A (bacteria; compost)

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: JIC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: B3A (bacteria; compost)

characteristic: Submitter Id

text: abandoned grassland

characteristic: current land use

text: grass

text: 0.1-0.3 m

text: Brachypodium 3rd gen

characteristic: description

text: UK; Norfolk; Bawburgh; JIC Church Farm

text: compost

text: 33.55 mg/kg

text: 3.49 mg/kg

text: doi: 10.1128/AEM.66.12.5488-5491.2000

text: soil metagenome

text: 120.5 mg/kg

text: 168.2 mg/kg

text: soil metagenome

text: Luvisol WRB

text: 16S rDNA

characteristic: target subfragment

text: loamy

characteristic: texture

text: B3A (bacteria)


B3B (bacteria; compost)

text: JIC

text: public

text: B3B (bacteria; compost)

text: abandoned grassland

text: grass

characteristic: current vegetation

text: 0.1-0.3 m

characteristic: depth

text: Brachypodium 3rd gen

text: UK; Norfolk; Bawburgh; JIC Church Farm

characteristic: geographic location (country and/or sea, region)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: compost

text: 33.55 mg/kg

characteristic: magnesium

text: 3.49 mg/kg

characteristic: nitrate

text: doi: 10.1128/AEM.66.12.5488-5491.2000

characteristic: nucleic acid extraction

characteristic: organic matter

text: soil metagenome

characteristic: organism

characteristic: pcr conditions

text: 120.5 mg/kg

characteristic: phosphate

text: 168.2 mg/kg

characteristic: potassium

text: soil metagenome

text: Luvisol WRB

text: 16S rDNA

text: loamy

text: B3B (bacteria)

characteristic: title


B3C (bacteria; compost)

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: JIC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: B3C (bacteria; compost)

characteristic: Submitter Id

text: abandoned grassland

characteristic: current land use

text: grass

text: 0.1-0.3 m

text: Brachypodium 3rd gen

characteristic: description

text: UK; Norfolk; Bawburgh; JIC Church Farm

text: compost

text: 33.55 mg/kg

text: 3.49 mg/kg

text: doi: 10.1128/AEM.66.12.5488-5491.2000

text: soil metagenome

text: 120.5 mg/kg

text: 168.2 mg/kg

text: soil metagenome

text: Luvisol WRB

characteristic: soil type

text: 16S rDNA

characteristic: target gene

text: loamy

text: B3C (bacteria)


Br1A (bacteria; compost)

text: JIC

text: public

text: Br1A (bacteria; compost)

text: abandoned grassland

text: grass

characteristic: current vegetation

text: 0.1-0.3 m

characteristic: depth

text: Brassica rapa 1st gen

text: UK; Norfolk; Bawburgh; JIC Church Farm

characteristic: geographic location (country and/or sea, region)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: compost

text: 33.55 mg/kg

characteristic: magnesium

text: 3.49 mg/kg

characteristic: nitrate

text: doi: 10.1128/AEM.66.12.5488-5491.2000

characteristic: nucleic acid extraction

characteristic: organic matter

text: soil metagenome

characteristic: organism

characteristic: pcr conditions

text: 120.5 mg/kg

characteristic: phosphate

text: 168.2 mg/kg

text: soil metagenome

text: Luvisol WRB

text: 16S rDNA

characteristic: target subfragment

text: loamy

characteristic: texture

text: Br1A (bacteria)


Br1B (bacteria; compost)

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: JIC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Br1B (bacteria; compost)

characteristic: Submitter Id

text: abandoned grassland

characteristic: current land use

text: grass

text: 0.1-0.3 m

text: Brassica rapa 1st gen

characteristic: description

text: UK; Norfolk; Bawburgh; JIC Church Farm

text: compost

text: 33.55 mg/kg

text: 3.49 mg/kg

text: doi: 10.1128/AEM.66.12.5488-5491.2000

text: soil metagenome

text: 120.5 mg/kg

text: 168.2 mg/kg

characteristic: potassium

text: soil metagenome

text: Luvisol WRB

text: 16S rDNA

text: loamy

text: Br1B (bacteria)

characteristic: title


Br1C (bacteria; compost)

text: JIC

text: public

text: Br1C (bacteria; compost)

text: abandoned grassland

text: grass

characteristic: current vegetation

text: 0.1-0.3 m

characteristic: depth

text: Brassica rapa 1st gen

text: UK; Norfolk; Bawburgh; JIC Church Farm

characteristic: geographic location (country and/or sea, region)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: compost

text: 33.55 mg/kg

characteristic: magnesium

text: 3.49 mg/kg

characteristic: nitrate

text: doi: 10.1128/AEM.66.12.5488-5491.2000

characteristic: nucleic acid extraction

characteristic: organic matter

text: soil metagenome

characteristic: organism

characteristic: pcr conditions

text: 120.5 mg/kg

characteristic: phosphate

text: 168.2 mg/kg

text: soil metagenome

text: Luvisol WRB

characteristic: soil type

text: 16S rDNA

characteristic: target gene

text: loamy

text: Br1C (bacteria)


Br2A (bacteria; compost)

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: JIC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Br2A (bacteria; compost)

characteristic: Submitter Id

text: abandoned grassland

characteristic: current land use

text: grass

text: 0.1-0.3 m

text: Brassica rapa 2nd gen

characteristic: description

text: UK; Norfolk; Bawburgh; JIC Church Farm

text: compost

text: 33.55 mg/kg

text: 3.49 mg/kg

text: doi: 10.1128/AEM.66.12.5488-5491.2000

text: soil metagenome

text: 120.5 mg/kg

text: 168.2 mg/kg

text: soil metagenome

text: Luvisol WRB

text: 16S rDNA

characteristic: target subfragment

text: loamy

characteristic: texture

text: Br2A (bacteria)


Br2B (bacteria; compost)

text: JIC

text: public

text: Br2B (bacteria; compost)

text: abandoned grassland

text: grass

characteristic: current vegetation

text: 0.1-0.3 m

characteristic: depth

text: Brassica rapa 2nd gen

text: UK; Norfolk; Bawburgh; JIC Church Farm

characteristic: geographic location (country and/or sea, region)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: compost

text: 33.55 mg/kg

characteristic: magnesium

text: 3.49 mg/kg

characteristic: nitrate

text: doi: 10.1128/AEM.66.12.5488-5491.2000

characteristic: nucleic acid extraction

characteristic: organic matter

text: soil metagenome

characteristic: organism

characteristic: pcr conditions

text: 120.5 mg/kg

characteristic: phosphate

text: 168.2 mg/kg

characteristic: potassium

text: soil metagenome

text: Luvisol WRB

text: 16S rDNA

text: loamy

text: Br2B (bacteria)

characteristic: title


Br2C (bacteria; compost)

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: JIC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Br2C (bacteria; compost)

characteristic: Submitter Id

text: abandoned grassland

characteristic: current land use

text: grass

text: 0.1-0.3 m

text: Brassica rapa 2nd gen

characteristic: description

text: UK; Norfolk; Bawburgh; JIC Church Farm

text: compost

text: 33.55 mg/kg

text: 3.49 mg/kg

text: doi: 10.1128/AEM.66.12.5488-5491.2000

text: soil metagenome

text: 120.5 mg/kg

text: 168.2 mg/kg

text: soil metagenome

text: Luvisol WRB

characteristic: soil type

text: 16S rDNA

characteristic: target gene

text: loamy

text: Br2C (bacteria)


Br3A (bacteria; compost)

text: JIC

text: public

text: Br3A (bacteria; compost)

text: abandoned grassland

text: grass

characteristic: current vegetation

text: 0.1-0.3 m

characteristic: depth

text: Brassica rapa 3rd gen

text: UK; Norfolk; Bawburgh; JIC Church Farm

characteristic: geographic location (country and/or sea, region)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: compost

text: 33.55 mg/kg

characteristic: magnesium

text: 3.49 mg/kg

characteristic: nitrate

text: doi: 10.1128/AEM.66.12.5488-5491.2000

characteristic: nucleic acid extraction

characteristic: organic matter

text: soil metagenome

characteristic: organism

characteristic: pcr conditions

text: 120.5 mg/kg

characteristic: phosphate

text: 168.2 mg/kg

text: soil metagenome

text: Luvisol WRB

text: 16S rDNA

characteristic: target subfragment

text: loamy

characteristic: texture

text: Br3A (bacteria)


Br3B (bacteria; compost)

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: JIC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Br3B (bacteria; compost)

characteristic: Submitter Id

text: abandoned grassland

characteristic: current land use

text: grass

text: 0.1-0.3 m

text: Brassica rapa 3rd gen

characteristic: description

text: UK; Norfolk; Bawburgh; JIC Church Farm

text: compost

text: 33.55 mg/kg

text: 3.49 mg/kg

text: doi: 10.1128/AEM.66.12.5488-5491.2000

text: soil metagenome

text: 120.5 mg/kg

text: 168.2 mg/kg

characteristic: potassium

text: soil metagenome

text: Luvisol WRB

text: 16S rDNA

text: loamy

text: Br3B (bacteria)

characteristic: title