text: Medical University of Vienna

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: human

text: Protocols: Mononuclear cells were isolated from peripheral blood or bone marrow using Ficoll. Genomic DNA (gDNA) was isolated from MNCs using QIAsymphony DSP DNA Midi Kit (Qiagen), MspI (New England Biolabs, NEB) digested, end-repaired and A-tailed using Klenow Polymerase (NEB) and dATP, dCTP and dGTP. Illumina adapters were ligated using Quick Ligase (NEB) followed by AMPure XP bead size selection (Beckman Coulter). RRBS libraries were bisulfite treated using EZ DNA MethylationDirect kit (Zymo Research) followed by quantification by qPCR. Enrichment PCR was performed using PfuTurbo Cx Hotstart kit (Agilent Technologies) followed by AMPure XP bead clean up. Quality of final libraries was checked by Experion analysis (BioRad).

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: BMA


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Medical University of Vienna

characteristic: INSDC last update

text: public

characteristic: SRA accession

tag: Namespace:Medical University of Vienna

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: human

characteristic: common name

characteristic: description

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Homo sapiens

text: BMB

characteristic: title

type: has member


text: Medical University of Vienna

text: public

text: ArrayExpress

text: human

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens


text: Medical University of Vienna

text: public

text: ArrayExpress

text: human

text: chronic myeloid leukemia

characteristic: disease

text: accelerated phase chronic myeloid leukemia

characteristic: disease staging

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens


text: Medical University of Vienna

characteristic: INSDC center name

text: public

text: ArrayExpress

text: human

text: chronic myeloid leukemia

text: blast crisis chronic myeloid leukemia

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens


text: Medical University of Vienna

characteristic: INSDC first public

text: public

text: ArrayExpress

text: human

text: chronic myeloid leukemia

text: chronic phase chronic myeloid leukemia

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens


text: Perio-UKM

text: public

characteristic: INSDC status

text: Acinetobacter baumannii

text: Acinetobacter baumannii


characteristic: ENA-CHECKLIST

text: Perio-UKM

text: public

text: not available

characteristic: collection date

text: Acinetobacter baumannii

text: Acinetobacter baumannii


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: GEORG-AUGUST UNIVERSITY GOETTINGEN

text: public

text: cattle

text: Carrier

text: Bos Taurus

text: Bos Taurus


characteristic: External Id

text: GEORG-AUGUST UNIVERSITY GOETTINGEN

characteristic: INSDC last update

text: public

text: cattle

text: Carrier

text: Bos Taurus

text: Bos Taurus

text: CDH


text: GEORG-AUGUST UNIVERSITY GOETTINGEN

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: cattle

text: Control

text: Bos Taurus

text: Bos Taurus

text: CDH


text: GEORG-AUGUST UNIVERSITY GOETTINGEN

text: public

text: cattle

characteristic: common name

text: affected

text: Bos Taurus

text: Bos Taurus

text: CDH


text: GEORG-AUGUST UNIVERSITY GOETTINGEN

text: public

text: cattle

text: Control

characteristic: description

text: Bos Taurus

text: Bos Taurus


text: public

tag: Namespace:Heilongjiang River Fisheries Research Institute of Chinese Academy of Fishery Sciences

text: goldfish

text: Carassius auratus

characteristic: organism

text: Carassius auratus

text: goldfish linkage map


text: Heilongjiang River Fisheries Research Institute of Chinese Academy of Fishery Sciences

text: public

text: goldfish

text: Carassius auratus

text: Carassius auratus

text: goldfish linkage map

characteristic: title


MAP-Sire

text: public

text: MAP-Sire

text: goldfish

text: Carassius auratus

text: Carassius auratus

text: goldfish linkage map


MAP-Dam

text: public

text: MAP-Dam

text: goldfish

text: Carassius auratus

text: Carassius auratus

text: goldfish linkage map


Enterobacter cloacae

text: URMITE

text: public

text: Enterobacter cloacae

text: Evolution of colistin heteroresistance

text: Enterobacter cloacae

text: Enterobacter cloacae

characteristic: strain

text: Colistin heteroresistance


text: public

tag: Namespace:Wellcome Trust Sanger Institute, Cambridge, UK EMBL-EBI, Cambridge, UK

text: ArrayExpress

characteristic: broker name

text: house mouse

text: Protocols: Embryos were recovered from superovulated F1 (C57Bl6xCBA) mice into M2 media supplemented with 4% BSA, as described previously25. Individual blastomeres were collected at late 2-cell (48 hours after hCG), late 4-cell (10 hours after the first 2-cell blastomere divided), late 8-cell (10 hours after the first 4-cell blastomere divided), 16-cell (78 hours after hCG) and 32-cell (86 hours after hCG) stages. Embryos for blastomere isolation at the 2-, 4,- and 8-cell stages were all collected at the 2-cell stage and cultured in KSOM media (Millipore) at 37C with 5% CO2. Embryos for the 16- and 32-cell blastomeres were collected directly at the respective stages. The zona pellucida of individual embryos was removed using Tyrode's solution (Sigma). Zona-free embryos were incubated for 5 minutes (for the 2,4 and 8-cell stages) or 20 minutes (for the 16 and 32-cell stages) in Ca2+ and Mg2+ free M2 before disaggregation by careful pipetting with a flame-polished glass pipette. Each embryo was processed individually. Single blastomeres for all stages were placed into individual tubes containing 2.3 l of 0.2 % Triton X-100 (Sigma) supplemented with 1 U/l RNAsIN (Ambion). Multiplex sequencing libraries were generated from amplified cDNA using Nextera XT (Illumina) and sequenced on a HiSeq 2500 running in rapid mode.

text: cleavage 16-cell

characteristic: developmental stage

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: OK

characteristic: single cell well quality


text: Wellcome Trust Sanger Institute, Cambridge, UK EMBL-EBI, Cambridge, UK

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: ArrayExpress

text: house mouse

text: cleavage 16-cell

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: OK


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: ArrayExpress

text: house mouse

text: cleavage 2-cell

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: OK


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: ArrayExpress

text: house mouse

text: cleavage 32-cell

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: OK


characteristic: INSDC last update

text: public

characteristic: SRA accession

text: ArrayExpress

text: house mouse

text: cleavage 32-cell

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: not OK


text: public

characteristic: Submitter Id

text: ArrayExpress

text: house mouse

characteristic: common name

text: cleavage 8-cell

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: OK


text: public

text: ArrayExpress

text: house mouse

text: Protocols: Embryos were recovered from superovulated F1 (C57Bl6xCBA) mice into M2 media supplemented with 4% BSA, as described previously25. Individual blastomeres were collected at late 2-cell (48 hours after hCG), late 4-cell (10 hours after the first 2-cell blastomere divided), late 8-cell (10 hours after the first 4-cell blastomere divided), 16-cell (78 hours after hCG) and 32-cell (86 hours after hCG) stages. Embryos for blastomere isolation at the 2-, 4,- and 8-cell stages were all collected at the 2-cell stage and cultured in KSOM media (Millipore) at 37C with 5% CO2. Embryos for the 16- and 32-cell blastomeres were collected directly at the respective stages. The zona pellucida of individual embryos was removed using Tyrode's solution (Sigma). Zona-free embryos were incubated for 5 minutes (for the 2,4 and 8-cell stages) or 20 minutes (for the 16 and 32-cell stages) in Ca2+ and Mg2+ free M2 before disaggregation by careful pipetting with a flame-polished glass pipette. Each embryo was processed individually. Single blastomeres for all stages were placed into individual tubes containing 2.3 l of 0.2 % Triton X-100 (Sigma) supplemented with 1 U/l RNAsIN (Ambion). At the late 2-cell stage one blastomere was injected with rhodamine-dextran (Invitrogen), as described previously. Embryos were observed at 20 minute intervals to categorise the order of divisions and whether each cleavage was meridional (M) or equatorial (E), relative to the position of the second polar body. 4-cell stage embryos were sorted into ME, EM, EE and MM groups. Embryos in which the polar body was absent were discarded. Following disaggregation, the individual cells were inspected under an epifluorescent inverted microscope and classified as originating from the first- or second-dividing 2-cell stage blastomere based on the rhodamine fluorescence. Multiplex sequencing libraries were generated from amplified cDNA using Nextera XT (Illumina) and sequenced on a HiSeq 2500 running in rapid mode.

characteristic: description

text: cleavage 4-cell

text: Equatorial - Equatorial

characteristic: division pattern

text: Mus musculus

characteristic: organism

text: Mus musculus

text: Mus musculus

text: OK


text: public

text: ArrayExpress

characteristic: broker name

text: house mouse

text: cleavage 4-cell

text: Equatorial - Meridional

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: OK

characteristic: title


text: public

text: ArrayExpress

text: house mouse

text: cleavage 4-cell

characteristic: developmental stage

text: Meridional - Equatorial

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ArrayExpress

text: house mouse

text: cleavage 4-cell

text: Meridional - Meridional

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: OK


text: Teladorsagia circumcincta

characteristic: ArrayExpress-Species

text: Tci2 (Weybridge)

characteristic: ArrayExpress-StrainOrLine

characteristic: ENA-CHECKLIST

text: SC

text: public

characteristic: INSDC status

text: Teladorsagia circumcincta

text: Teladorsagia circumcincta

text: gDNA extracted from thousands of L3.

characteristic: sample description

text: Teladorsagia circumcincta

text: Tci2 (Weybridge)

characteristic: strain


text: MEF

characteristic: ArrayExpress-CellType

text: Mus musculus

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: house mouse

text: Mus musculus

text: MEF RNA

text: Mus musculus


Protochlamydia greubae

text: URMITE

text: public

text: Protochlamydia greubae

text: Protochlamydia greubae was isolated from a soil sample in the Bouches du Rhones region-France by co-culture with Acanthamoeba Castellanii (A. castellanii strain Neff 30010)

characteristic: description

text: Parachlamydia sp. C2

characteristic: organism

text: Parachlamydia sp. C2

text: Protochlamydia greubae genome

characteristic: title


characteristic: INSDC center name

characteristic: INSDC first public

text: public

tag: Namespace:ANIMAL HEALTH AND VETERINARY LABORATORIES AGENCY

characteristic: collection date

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: Porcine

characteristic: host

text: intestine

characteristic: isolation source

text: Salmonella enterica

text: Salmonella enterica

text: Typhimurium var. Copenhagen

characteristic: serovar

text: Colistin resistant isolate


characteristic: ENA-CHECKLIST

text: ANIMAL HEALTH AND VETERINARY LABORATORIES AGENCY

text: public

characteristic: INSDC status

text: United Kingdom

text: Porcine

text: intestine

text: Escherichia coli

text: Escherichia coli

characteristic: serotype

text: Colistin resistant isolate


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: United Kingdom

text: lab strain

text: Escherichia coli

text: Escherichia coli

text: Recipient Lab strain


characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: United Kingdom

text: Escherichia coli

text: Escherichia coli

text: Transconjugant


text: PNRI

text: public

text: Baker's Yeast

characteristic: common name

text: RAD-seq reduced-genome sequencing of yeast strain for phylogenetic analysis

characteristic: description

text: Saccharomyces cerevisiae

characteristic: organism

text: Saccharomyces cerevisiae

characteristic: title


text: NATIONAL VETERINARY INSTITUTE (SVA)

characteristic: INSDC center name

characteristic: INSDC first public

text: public

tag: Namespace:NATIONAL VETERINARY INSTITUTE (SVA)

text: Paenibacillus larvae

text: Paenibacillus larvae

text: Outbreak isolate 2014


text: UNIVERSITY OF MANCHESTER

text: public

tag: Namespace:UNIVERSITY OF MANCHESTER

text: Cloned partial DNA sequence of the trn-L tRNA gene amplified from a DNA extract of a pottery swab sample.

text: Fabaceae environmental sample

text: Fabaceae environmental sample


text: UNIVERSITY OF MANCHESTER

text: public

text: Cloned partial trn-L tRNA gene DNA sequence, amplified from the DNA extract of a pottery swab sample.

text: Fabaceae environmental sample

text: Fabaceae environmental sample


text: UNIVERSITY OF MANCHESTER

text: public

text: fava bean

text: Vicia faba

text: Vicia faba


text: UNIVERSITY OF MANCHESTER

text: public

text: fava bean

text: Cloned partial trn-L tRNA gene DNA sequence, amplified from the DNA extract of a pottery swab sample

text: Vicia faba

text: Vicia faba


text: UNIVERSITY OF MANCHESTER

text: public

text: fava bean

text: Cloned partial trn-L tRNA DNA sequence, amplified from the DNA extract of a pottery swab sample.

text: Vicia faba

text: Vicia faba


text: UNIVERSITY OF MANCHESTER

text: public

text: Cloned non-specifically amplified DNA sequence, amplified from the DNA extract of a pottery swab sample

text: unidentified

text: unidentified


text: UNIVERSITY OF MANCHESTER

text: public

text: Fabaceae environmental sample

text: Fabaceae environmental sample


text: public

tag: Namespace:UNIVERSITY OF BRITISH COLUMBIA, DEPT BOTANY

text: freshwater sediment metagenome

text: freshwater sediment metagenome


text: UNIVERSITY OF BRITISH COLUMBIA, DEPT BOTANY

text: public

text: freshwater sediment metagenome

text: freshwater sediment metagenome


text: public

tag: Namespace:ZHEJIANG UNIVERSITY, HANGZHOU, P. R. CHINA

text: 30, 60, 120, 180 days

characteristic: dev stage

text: gut metagenome

text: gut metagenome

text: Ceacal samples from rabbit


text: ZHEJIANG UNIVERSITY, HANGZHOU, P. R. CHINA

text: public

text: 30, 60, 120, 180 days

text: gut metagenome

text: gut metagenome

text: Ceacal samples from rabbit


text: QLD Health

text: public

tag: Namespace:QLD Health

characteristic: collection date

text: Australia

characteristic: geographic location (country and/or sea)

text: diseased

characteristic: host health state

text: natural

characteristic: host scientific name

text: homo sapiens

characteristic: isolate

text: urine

characteristic: isolation source

text: Neisseria gonorrhoeae

text: Neisseria gonorrhoeae

text: Ng


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: INSDC status

tag: Namespace:DNA SEQUENCING AND GENOMICS LABORATORY, INSTITUTE OF BIOTECHNOLOGY, UNIVERSITY OF HELSINKI

text: Hepatocellular

characteristic: cell type

text: human

text: Homo sapiens

text: Homo sapiens

text: Huh-7 JCRB0403


characteristic: INSDC last update

text: public

characteristic: SRA accession

tag: Namespace:Department of Plant Sciences, University of Cambridge, UK

characteristic: Submitter Id

unit: day

characteristic: age

text: ArrayExpress

characteristic: broker name

text: Protocols: QIAGEN RNeasy Plant Mini kit Growth Conditions Treatment Conditions TruSeq RNA v2

characteristic: description

text: Eitolated

characteristic: growth condition

text: Cleome gynandra

characteristic: organism

text: Cleome gynandra

text: Cleome gynandra


text: Department of Plant Sciences, University of Cambridge, UK

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ArrayExpress

text: Eitolated

text: Cleome gynandra

text: Cleome gynandra

text: Cleome gynandra

characteristic: title


characteristic: ENA-CHECKLIST

text: public

text: ArrayExpress

text: Eitolated + 6h white light 100 mol m2 s1ol m2 s1

text: Cleome gynandra

text: Cleome gynandra

text: Cleome gynandra


characteristic: ENA-FIRST-PUBLIC

text: public

tag: Namespace:Genome-Scale Biology Program, Research Programs Unit and Department of Pathology, Haartman Institute, University of Helsinki

text: ArrayExpress

characteristic: cell line

text: human

characteristic: common name

text: Protocols: BC-3 cells were grown on RPMI 1640 medium supplemented with 15% FCS (Invitrogen), 100 U/ml penicillin G, and 100 g/ml streptomycin BC-3 cells were cross-linked with 1% formaldehyde in PBS at room temperature for 10min. After a wash in PBS containing 125 mM glycine, cells were collected by centrifugation and sonicated in lysis buffer containing 0,1% SDS, 0,1% sodium deoxycholate, 1 mM EDTA, 10 mM TrisHCl pH 8.0, 140 mM NaCl, 1% Triton X-100 and protease inhibitors to generate chromatin fragments of 100300 bp in length. Following a brief centrifugation (16.000xg, 20 min, 4 C), the fragmented chromatin was immunoprecipitated with a monoclonal antibody against p53 (Clone DO-1, GeneSpin) or control IgG (normal mouse IgG: sc-2025, Santa-Cruz Biotechnology). The precipitates were incubated at 65 C over night to reverse the formaldehyde crosslinking and samples incubated with proteinase K and RNase-A before phenol extraction and ethanol-precipitation of target DNA. Similarly as in Tuupanen et al. Nature Genetics 2009.PMID: 19561604 BC-3 cells were cross-linked with 1% formaldehyde in PBS at room temperature for 10min. After a wash in PBS containing 125 mM glycine, cells were collected by centrifugation and sonicated in lysis buffer containing 0,1% SDS, 0,1% sodium deoxycholate, 1 mM EDTA, 10 mM TrisHCl pH 8.0, 140 mM NaCl, 1% Triton X-100 and protease inhibitors to generate chromatin fragments of 100300 bp in length. Following a brief centrifugation (16.000xg, 20 min, 4 C), the fragmented chromatin was immunoprecipitated with a monoclonal antibody against p53 (Clone DO-1, GeneSpin) or control IgG (normal mouse IgG: sc-2025, Santa-Cruz Biotechnology). The precipitates were incubated at 65 C over night to reverse the formaldehyde crosslinking and samples incubated with proteinase K and RNase-A before phenol extraction and ethanol-precipitation of target DNA. IP-DNA fragments were first repaired using Klenow and T4 DNA polymerases and T4 polynucleotide kinase (MBI Fermentas, Latvia), and then ligated to adapters according to manufacturer's instructions (Illumina). PCR-amplified fragments of approximately 180-300bp were isolated.

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Genome-Scale Biology Program, Research Programs Unit and Department of Pathology, Haartman Institute, University of Helsinki

text: public

characteristic: INSDC status

text: ArrayExpress

text: human

text: 12-O-Tetradecanoylphorbol-13-acetate (TPA)

characteristic: compound

text: Protocols: BC-3 cells were grown on RPMI 1640 medium supplemented with 15% FCS (Invitrogen), 100 U/ml penicillin G, and 100 g/ml streptomycin BC-3 cells were cross-linked with 1% formaldehyde in PBS at room temperature for 10min. After a wash in PBS containing 125 mM glycine, cells were collected by centrifugation and sonicated in lysis buffer containing 0,1% SDS, 0,1% sodium deoxycholate, 1 mM EDTA, 10 mM TrisHCl pH 8.0, 140 mM NaCl, 1% Triton X-100 and protease inhibitors to generate chromatin fragments of 100300 bp in length. Following a brief centrifugation (16.000xg, 20 min, 4 C), the fragmented chromatin was immunoprecipitated with a monoclonal antibody against p53 (Clone DO-1, GeneSpin) or control IgG (normal mouse IgG: sc-2025, Santa-Cruz Biotechnology). The precipitates were incubated at 65 C over night to reverse the formaldehyde crosslinking and samples incubated with proteinase K and RNase-A before phenol extraction and ethanol-precipitation of target DNA. Similarly as in Tuupanen et al. Nature Genetics 2009.PMID: 19561604 TPA was added to the cells for 4h or 24h with 20 ng/ml dose BC-3 cells were cross-linked with 1% formaldehyde in PBS at room temperature for 10min. After a wash in PBS containing 125 mM glycine, cells were collected by centrifugation and sonicated in lysis buffer containing 0,1% SDS, 0,1% sodium deoxycholate, 1 mM EDTA, 10 mM TrisHCl pH 8.0, 140 mM NaCl, 1% Triton X-100 and protease inhibitors to generate chromatin fragments of 100300 bp in length. Following a brief centrifugation (16.000xg, 20 min, 4 C), the fragmented chromatin was immunoprecipitated with a monoclonal antibody against p53 (Clone DO-1, GeneSpin) or control IgG (normal mouse IgG: sc-2025, Santa-Cruz Biotechnology). The precipitates were incubated at 65 C over night to reverse the formaldehyde crosslinking and samples incubated with proteinase K and RNase-A before phenol extraction and ethanol-precipitation of target DNA. IP-DNA fragments were first repaired using Klenow and T4 DNA polymerases and T4 polynucleotide kinase (MBI Fermentas, Latvia), and then ligated to adapters according to manufacturer's instructions (Illumina). PCR-amplified fragments of approximately 180-300bp were isolated.

unit: nanogram per milliliter

characteristic: dose

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

unit: hour

characteristic: time


characteristic: INSDC last update

text: public

characteristic: SRA accession

text: E-MTAB-4356:Balistreri_mIgG_control_Nutlin

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: human

text: Protocols: BC-3 cells were grown on RPMI 1640 medium supplemented with 15% FCS (Invitrogen), 100 U/ml penicillin G, and 100 g/ml streptomycin BC-3 cells were cross-linked with 1% formaldehyde in PBS at room temperature for 10min. After a wash in PBS containing 125 mM glycine, cells were collected by centrifugation and sonicated in lysis buffer containing 0,1% SDS, 0,1% sodium deoxycholate, 1 mM EDTA, 10 mM TrisHCl pH 8.0, 140 mM NaCl, 1% Triton X-100 and protease inhibitors to generate chromatin fragments of 100300 bp in length. Following a brief centrifugation (16.000xg, 20 min, 4 C), the fragmented chromatin was immunoprecipitated with a monoclonal antibody against p53 (Clone DO-1, GeneSpin) or control IgG (normal mouse IgG: sc-2025, Santa-Cruz Biotechnology). The precipitates were incubated at 65 C over night to reverse the formaldehyde crosslinking and samples incubated with proteinase K and RNase-A before phenol extraction and ethanol-precipitation of target DNA. Similarly as in Tuupanen et al. Nature Genetics 2009.PMID: 19561604 Nutlin 3 treatment for 8h with 10 M concentration. BC-3 cells were cross-linked with 1% formaldehyde in PBS at room temperature for 10min. After a wash in PBS containing 125 mM glycine, cells were collected by centrifugation and sonicated in lysis buffer containing 0,1% SDS, 0,1% sodium deoxycholate, 1 mM EDTA, 10 mM TrisHCl pH 8.0, 140 mM NaCl, 1% Triton X-100 and protease inhibitors to generate chromatin fragments of 100300 bp in length. Following a brief centrifugation (16.000xg, 20 min, 4 C), the fragmented chromatin was immunoprecipitated with a monoclonal antibody against p53 (Clone DO-1, GeneSpin) or control IgG (normal mouse IgG: sc-2025, Santa-Cruz Biotechnology). The precipitates were incubated at 65 C over night to reverse the formaldehyde crosslinking and samples incubated with proteinase K and RNase-A before phenol extraction and ethanol-precipitation of target DNA. IP-DNA fragments were first repaired using Klenow and T4 DNA polymerases and T4 polynucleotide kinase (MBI Fermentas, Latvia), and then ligated to adapters according to manufacturer's instructions (Illumina). PCR-amplified fragments of approximately 180-300bp were isolated.

unit: micromolar

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens


characteristic: INSDC center name

text: public

text: ArrayExpress

text: human

characteristic: description

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Homo sapiens


characteristic: ENA-CHECKLIST

characteristic: INSDC first public

text: public

text: E-MTAB-4356:Balistreri_p53_sample_Nutlin

text: ArrayExpress

text: human

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

characteristic: title


Cellulomonas timonensis

characteristic: ENA-FIRST-PUBLIC

text: URMITE

text: public

text: Cellulomonas timonensis

text: Culturomics study of the human gut microbiota

text: Cellulomonas sp. SN7

text: Cellulomonas sp. SN7

characteristic: strain

text: Cellulomonas timonensis genome


Collinsella ihumii

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: URMITE

text: public

text: Collinsella ihumii

text: Culturomics study of the human gut

text: Collinsella sp. GD7

text: Collinsella sp. GD7

text: Collinsella ihumii genome


text: public

characteristic: INSDC status

tag: Namespace:Austrian Agency for Health and Food Safety

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate L1512


text: Austrian Agency for Health and Food Safety

text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate SLCC546


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate SLCC640


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate SLCC661


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate SLCC757


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate SLCC2470


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate SLCC2499


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate SLCC2545


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate SLCC2588


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate SLCC2645


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate SLCC2818


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate L0412


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate MRL1400761


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate MRL1400762


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate MRL1400850


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate MRL1401054


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate SLCC196


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate SLCC197


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate L3013


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate L1513


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate MRL1500016


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate MRL1400616


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate MRL1401315


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate MRL1500034


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate L1213


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate L1712


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate MRL1300816


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate MRL1400618


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate MRL1400658


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate MRL1400997


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate MRL1500093


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate SLCC174


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate SLCC207


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate SLCC520


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate SLCC646


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate SLCC671


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate SLCC1444


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate SLCC2209


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate SLCC5405


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate SLCC5406


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate L1412


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate L3012


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate L3112


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate SLCC531


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate SLCC532


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate SLCC589


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate SLCC676


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate SLCC684


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate SLCC1092


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate SLCC1144


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate SLCC1306


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate SLCC1499


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate SLCC1572


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate SLCC1574


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate SLCC1598


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate SLCC1631


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate SLCC1669


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate SLCC1676


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate SLCC1747


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate SLCC2129


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate SLCC2295


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate SLCC2896


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate L2312


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate L2513


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate MRL1400615


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate MRL1500014


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate SLCC1003


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate L0912


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate L2612


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate MRL1400954


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate L0112


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate SLCC191


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate SLCC645


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate SLCC2261


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate SLCC1589


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate SLCC993


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate MRL1400459


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate MRL1400747


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate L2812


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate L1313


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate MRL1400659


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate MRL1400683


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate L3513


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate SLCC685


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate MRL1401314


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate MRL1401209


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate L2412


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate SLCC1876


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate SLCC187


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate SLCC518


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate SLCC534


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate SLCC635


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate SLCC710


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate SLCC2781


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate 11020515001


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate 11072355001


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate 94000610


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate 94002911


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate L0512


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate MRL1200494


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate MRL1300218


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate MRL1300219


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate MRL1300297


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate MRL1400532


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate MRL1400991


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate MRL1500324


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate L3512


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate MRL1500341


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate SLCC192


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate SLCC222


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate SLCC1672


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate SLCC1673


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate SLCC2655


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate SLCC887


text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: isolate SLCC2956


text: TUGRAZ

text: public

characteristic: collection date

text: dessert banana

characteristic: common name

text: Rhizosphere

characteristic: environment (biome)

text: non-infested

characteristic: environment (feature)

text: Soil

characteristic: environment (material)

text: Nicaragua

characteristic: geographic location (country and/or sea)

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: Musa acuminata AAA Group

text: plant-associated

characteristic: plant-associated environmental package

characteristic: project name

text: Musa acuminata AAA Group

text: Illumina MiSeq

characteristic: sequencing method


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: TUGRAZ

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: dessert banana

text: Rhizosphere

text: infested

text: Soil

text: Nicaragua

text: metagenome

text: Musa acuminata AAA Group

characteristic: organism

text: plant-associated

text: Musa acuminata AAA Group

text: Illumina MiSeq

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: TUGRAZ

text: public

characteristic: INSDC status

characteristic: collection date

text: dessert banana

characteristic: common name

text: Endorhiza

characteristic: environment (biome)

text: non-infested

characteristic: environment (feature)

text: Root

characteristic: environment (material)

text: Nicaragua

text: metagenome

text: Musa acuminata AAA Group

text: plant-associated

text: Musa acuminata AAA Group

text: Illumina MiSeq


characteristic: ENA-CHECKLIST

text: TUGRAZ

text: public

text: dessert banana

text: Endorhiza

text: infested

text: Root

text: Nicaragua

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: Musa acuminata AAA Group

text: plant-associated

characteristic: plant-associated environmental package

characteristic: project name

text: Musa acuminata AAA Group

text: Illumina MiSeq

characteristic: sequencing method


characteristic: ENA-FIRST-PUBLIC

text: TUGRAZ

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: dessert banana

text: Pseudostem

text: non-infested

text: Pseudostem

text: Nicaragua

text: metagenome

text: Musa acuminata AAA Group

characteristic: organism

text: plant-associated

text: Musa acuminata AAA Group

text: Illumina MiSeq

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: TUGRAZ

text: public

characteristic: INSDC status

characteristic: collection date

text: dessert banana

characteristic: common name

text: Pseudostem

characteristic: environment (biome)

text: infested

characteristic: environment (feature)

text: Pseudostem

characteristic: environment (material)

text: Nicaragua

text: metagenome

text: Musa acuminata AAA Group

text: plant-associated

text: Musa acuminata AAA Group

text: Illumina MiSeq


characteristic: ENA-CHECKLIST

text: TUGRAZ

text: public

text: dessert banana

text: Phyllosphere

text: non-infested

text: Leaves

text: Nicaragua

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: Musa acuminata AAA Group

text: plant-associated

characteristic: plant-associated environmental package

characteristic: project name

text: Musa acuminata AAA Group

text: Illumina MiSeq

characteristic: sequencing method


characteristic: ENA-FIRST-PUBLIC

text: TUGRAZ

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: dessert banana

text: Phyllosphere

text: infested

text: Leaves

text: Nicaragua

text: metagenome

text: Musa acuminata AAA Group

characteristic: organism

text: plant-associated

text: Musa acuminata AAA Group

text: Illumina MiSeq

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: TUGRAZ

text: public

characteristic: INSDC status

characteristic: collection date

text: dessert banana

characteristic: common name

text: Rhizosphere

characteristic: environment (biome)

text: non-infested

characteristic: environment (feature)

text: Soil

characteristic: environment (material)

text: Costa Rica

text: metagenome

text: Musa acuminata AAA Group

text: plant-associated

text: Musa acuminata AAA Group

text: Illumina MiSeq


characteristic: ENA-CHECKLIST

text: TUGRAZ

text: public

text: dessert banana

text: Rhizosphere

text: infested

text: Soil

text: Costa Rica

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: Musa acuminata AAA Group

text: plant-associated

characteristic: plant-associated environmental package

characteristic: project name

text: Musa acuminata AAA Group

text: Illumina MiSeq

characteristic: sequencing method


characteristic: ENA-FIRST-PUBLIC

text: TUGRAZ

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: dessert banana

text: Endorhiza

text: non-infested

text: Root

text: Costa Rica

text: metagenome

text: Musa acuminata AAA Group

characteristic: organism

text: plant-associated

text: Musa acuminata AAA Group

text: Illumina MiSeq

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: TUGRAZ

text: public

characteristic: INSDC status

characteristic: collection date

text: dessert banana

characteristic: common name

text: Endorhiza

characteristic: environment (biome)

text: infested

characteristic: environment (feature)

text: Root

characteristic: environment (material)

text: Costa Rica

text: metagenome

text: Musa acuminata AAA Group

text: plant-associated

text: Musa acuminata AAA Group

text: Illumina MiSeq


characteristic: ENA-CHECKLIST

text: TUGRAZ

text: public

text: dessert banana

text: Pseudostem

text: non-infested

text: Pseudostem

text: Costa Rica

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: Musa acuminata AAA Group

text: plant-associated

characteristic: plant-associated environmental package

characteristic: project name

text: Musa acuminata AAA Group

text: Illumina MiSeq

characteristic: sequencing method


characteristic: ENA-FIRST-PUBLIC

text: TUGRAZ

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: dessert banana

text: Pseudostem

text: infested

text: Pseudostem

text: Costa Rica

text: metagenome

text: Musa acuminata AAA Group

characteristic: organism

text: plant-associated

text: Musa acuminata AAA Group

text: Illumina MiSeq

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: TUGRAZ

text: public

characteristic: INSDC status

characteristic: collection date

text: dessert banana

characteristic: common name

text: Phyllosphere

characteristic: environment (biome)

text: non-infested

characteristic: environment (feature)

text: Leaves

characteristic: environment (material)

text: Costa Rica

text: metagenome

text: Musa acuminata AAA Group

text: plant-associated

text: Musa acuminata AAA Group

text: Illumina MiSeq


characteristic: ENA-CHECKLIST

text: TUGRAZ

text: public

text: dessert banana

text: Phyllosphere

text: infested

text: Leaves

text: Costa Rica

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: Musa acuminata AAA Group

text: plant-associated

characteristic: plant-associated environmental package

characteristic: project name

text: Musa acuminata AAA Group

text: Illumina MiSeq

characteristic: sequencing method


characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF MELBOURNE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: SRA accession

tag: Namespace:UNIVERSITY OF MELBOURNE

characteristic: Submitter Id

text: New Zealand

text: Human

characteristic: host

text: Wound

characteristic: isolation source

text: Staphylococcus aureus

characteristic: organism

text: Staphylococcus aureus

characteristic: title


Xanthomonas massiliensis

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: URMITE

text: public

characteristic: INSDC status

text: Xanthomonas massiliensis

text: Culturomics study of the human gut

characteristic: description

text: Xanthomonas sp. SN8

text: Xanthomonas sp. SN8

characteristic: strain

text: Xanthomonas massiliensis genome


Paenibacillus phoceensis

text: URMITE

text: public

text: Paenibacillus phoceensis

text: Culturomics study of the human gut

text: Paenibacillus sp. MT24

text: Paenibacillus sp. MT24

text: Paenibacillus phoceensis genome


text: CIPF

text: public

text: human

characteristic: common name

text: Homo sapiens

text: Homo sapiens


characteristic: ENA-FIRST-PUBLIC

text: public

tag: Namespace:University of Illinois at Urbana Champaign

text: ArrayExpress

characteristic: broker name

text: house mouse

text: Protocols: Silastic tubing containing 35 ul of oil or 35ul of oil with 180ug/ml E2. Implants remained in mice for 7 days. After 7 days, mice were placed in a hypoxia chamber (BioSpherix, Lacona, NY) for 3 hours, which was equilibrated to 7% O2 and 93% N2. Mice and O2 concentrations were monitored continuously throughout the experiment. Mice were returned to normoxic conditions for 1 hour and then sacrificed. The brains were dissected and the cerebral cortices were harvested. Total RNA was extracted using Ambion RNAqueous according to manufacturer's protocol.Samples were treated with Turbo DNA-free reagent to remove genomic DNA. The TruSeq RNA sample prep kit was used according to manufacturer's protocol. 1ug total RNA was used to make poly-A selected and barcoded libraries. Libraries were pooled and quantifed using real time PCR and library quantification kit (Kapa Biosystems).

text: hypoxia (7% O2) for 3 hours with 1 hour of recovery

characteristic: growth condition

text: Mus musculus

text: Mus musculus

text: cerebral cortex

characteristic: organism part

text: Mus musculus

text: female

characteristic: sex


text: University of Illinois at Urbana Champaign

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: house mouse

text: Mus musculus

text: Mus musculus

text: cerebral cortex

text: Mus musculus

text: female


Enterobacter timonensis

characteristic: ENA-CHECKLIST

text: URMITE

text: public

text: Enterobacter timonensis

text: Culturomics study of the human gut

text: Enterobacter sp. MT20

characteristic: organism

text: Enterobacter sp. MT20

text: Enterobacter timonensis genome

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Research Associate

text: public

characteristic: INSDC status

tag: Namespace:Research Associate

text: ArrayExpress

text: embryonic stem cell

characteristic: cell type

text: house mouse

text: Protocols: R1 and E14Tg2 mouse ESCs were cultured on 0,1% gelatin in ESC medium: knock-out DMEM supplemented with 15% FBS (Sigma) and 1,000 U/ml LIF ESGRO (Millipore). mESCs were treated at indicated concentrations to activate the Wnt pathway: purified Wnt3a (Peprotech); BIO (Calbiochem); CHIR99021 (Calbiochem). BIO and CHIR99021 were resuspendend in DMSO (Sigma). Briefly, ESCs were trypsinised and crosslinked in 1% formaldehyde for 10 min at room temperature. Crosslinking was quenched with 0.125 M glycine for 5 min. The pelleted cells were lysed in 1 ml ChIP buffer and sonicated for 10 min in a Bioruptor sonicator (Diagenode). The soluble material was quantified using Bradford assays. To immunoprecipitate the transcription factors, 500 g protein was used. Antibodies were incubated overnight with the chromatin. The immunocomplexes were recovered with 30 l protein A or G agarose bead slurries. The immunoprecipitated material was washed three times with low-salt buffer and one time with high-salt buffer.

text: Mus musculus

text: Mus musculus

text: Mus musculus


tag: attribute

characteristic: ENA-FIRST-PUBLIC

text: Wellcome Trust Sanger Institute, Cambridge, UK EMBL-EBI, Cambridge, UK

text: public

characteristic: batch number

text: ArrayExpress

text: house mouse

characteristic: common name

text: Protocols: Embryos were recovered from superovulated F1 (C57Bl6xCBA) mice into M2 media supplemented with 4% BSA, as described previously25. Individual blastomeres were collected at late 2-cell (48 hours after hCG), late 4-cell (10 hours after the first 2-cell blastomere divided), late 8-cell (10 hours after the first 4-cell blastomere divided), 16-cell (78 hours after hCG) and 32-cell (86 hours after hCG) stages. Embryos for blastomere isolation at the 2-, 4,- and 8-cell stages were all collected at the 2-cell stage and cultured in KSOM media (Millipore) at 37C with 5% CO2. Embryos for the 16- and 32-cell blastomeres were collected directly at the respective stages. The zona pellucida of individual embryos was removed using Tyrode's solution (Sigma). Zona-free embryos were incubated for 5 minutes (for the 2,4 and 8-cell stages) or 20 minutes (for the 16 and 32-cell stages) in Ca2+ and Mg2+ free M2 before disaggregation by careful pipetting with a flame-polished glass pipette. Each embryo was processed individually. Single blastomeres for all stages were placed into individual tubes containing 2.3 l of 0.2 % Triton X-100 (Sigma) supplemented with 1 U/l RNAsIN (Ambion). Multiplex sequencing libraries were generated from amplified cDNA using Nextera XT (Illumina) and sequenced on a HiSeq 2500 running in rapid mode.

characteristic: description

text: cleavage 8-cell

characteristic: developmental stage

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: OK

characteristic: single cell well quality

text: 1 microliter of 1:250000

characteristic: spike in dilution


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: ArrayExpress

characteristic: broker name

text: house mouse

text: cleavage 2-cell

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: OK

text: 1 microliter of 1:1000000


characteristic: ENA-CHECKLIST

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: house mouse

text: Protocols: Embryos were recovered from superovulated F1 (C57Bl6xCBA) mice into M2 media supplemented with 4% BSA, as described previously25. Individual blastomeres were collected at late 2-cell (48 hours after hCG), late 4-cell (10 hours after the first 2-cell blastomere divided), late 8-cell (10 hours after the first 4-cell blastomere divided), 16-cell (78 hours after hCG) and 32-cell (86 hours after hCG) stages. Embryos for blastomere isolation at the 2-, 4,- and 8-cell stages were all collected at the 2-cell stage and cultured in KSOM media (Millipore) at 37C with 5% CO2. Embryos for the 16- and 32-cell blastomeres were collected directly at the respective stages. The zona pellucida of individual embryos was removed using Tyrode's solution (Sigma). Zona-free embryos were incubated for 5 minutes (for the 2,4 and 8-cell stages) or 20 minutes (for the 16 and 32-cell stages) in Ca2+ and Mg2+ free M2 before disaggregation by careful pipetting with a flame-polished glass pipette. Each embryo was processed individually. Single blastomeres for all stages were placed into individual tubes containing 2.3 l of 0.2 % Triton X-100 (Sigma) supplemented with 1 U/l RNAsIN (Ambion). At the late 2-cell stage one blastomere was injected with rhodamine-dextran (Invitrogen), as described previously. Embryos were observed at 20 minute intervals to categorise the order of divisions and whether each cleavage was meridional (M) or equatorial (E), relative to the position of the second polar body. 4-cell stage embryos were sorted into ME, EM, EE and MM groups. Embryos in which the polar body was absent were discarded. Following disaggregation, the individual cells were inspected under an epifluorescent inverted microscope and classified as originating from the first- or second-dividing 2-cell stage blastomere based on the rhodamine fluorescence. Multiplex sequencing libraries were generated from amplified cDNA using Nextera XT (Illumina) and sequenced on a HiSeq 2500 running in rapid mode.

text: cleavage 4-cell

text: Meridional - Equatorial

characteristic: division pattern

text: Mus musculus

characteristic: organism

text: Mus musculus

text: Mus musculus

text: OK

text: 1 microliter of 1:1000000


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: INSDC status

text: ArrayExpress

text: house mouse

text: cleavage 4-cell

text: Equatorial - Meridional

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: OK

text: 1 microliter of 1:1000000

characteristic: title


characteristic: ENA-FIRST-PUBLIC

text: LSHTM

text: public

text: Chlamydia trachomatis

text: Chlamydia trachomatis

text: C.tracomatis


text: Plasmodium Vivax

characteristic: ArrayExpress-Species

text: SC

text: public

text: malaria parasite P. vivax

characteristic: common name

text: Plasmodium vivax

text: Plasmodium vivax


name: Sequencing Reads for Sporomusa sphaeroides DSM 2875

text: UNIVERSITY OF WARWICK

text: public

tag: Namespace:UNIVERSITY OF WARWICK

text: Sporomusa sphaeroides DSM 2875

text: Sporomusa sphaeroides DSM 2875

text: Sequencing Reads for Draft Genome Sequence of Sporomusa sphaeroides DSM 2875


K1 (healthy saliva sample)

text: public

text: K1 (healthy saliva sample)

tag: Namespace:Faculty of Dentistry,Departtment of Oral Biology

characteristic: collection date

text: human

text: saliva

characteristic: environment (biome)

text: oral

characteristic: environment (feature)

text: DNA isolated from saliva

characteristic: environment (material)

text: Norway

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: human-oral

characteristic: humam oral environmental package

text: metagenome

characteristic: investigation type

text: Homo sapiens

text: 16S rDNA saliva study

characteristic: project name

text: Homo sapiens

text: Pyrosequencing

characteristic: sequencing method

text: Whole unstimulated saliva samples


K2 (healthy saliva sample)

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Faculty of Dentistry,Departtment of Oral Biology

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: K2 (healthy saliva sample)

characteristic: Submitter Id

text: human

text: saliva

text: oral

text: DNA isolated from saliva

text: Norway

text: human-oral

text: metagenome

text: Homo sapiens

characteristic: organism

text: 16S rDNA saliva study

text: Homo sapiens

text: Pyrosequencing

text: Whole unstimulated saliva samples


K3 (healthy saliva sample)

characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: INSDC status

text: K3 (healthy saliva sample)

characteristic: collection date

text: human

characteristic: common name

text: saliva

characteristic: environment (biome)

text: oral

characteristic: environment (feature)

text: DNA isolated from saliva

characteristic: environment (material)

text: Norway

text: human-oral

text: metagenome

text: Homo sapiens

text: 16S rDNA saliva study

text: Homo sapiens

text: Pyrosequencing

text: Whole unstimulated saliva samples

characteristic: title


K6 (healthy saliva sample)

characteristic: ENA-CHECKLIST

text: public

text: K6 (healthy saliva sample)

text: human

text: saliva

text: oral

text: DNA isolated from saliva

text: Norway

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: human-oral

characteristic: humam oral environmental package

text: metagenome

characteristic: investigation type

text: Homo sapiens

text: 16S rDNA saliva study

characteristic: project name

text: Homo sapiens

text: Pyrosequencing

characteristic: sequencing method

text: Whole unstimulated saliva samples


K7 (healthy saliva sample)

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: K7 (healthy saliva sample)

characteristic: Submitter Id

text: human

text: saliva

text: oral

text: DNA isolated from saliva

text: Norway

text: human-oral

text: metagenome

text: Homo sapiens

characteristic: organism

text: 16S rDNA saliva study

text: Homo sapiens

text: Pyrosequencing

text: Whole unstimulated saliva samples


K8 (healthy saliva sample)

characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: INSDC status

text: K8 (healthy saliva sample)

characteristic: collection date

text: human

characteristic: common name

text: saliva

characteristic: environment (biome)

text: oral

characteristic: environment (feature)

text: DNA isolated from saliva

characteristic: environment (material)

text: Norway

text: human-oral

text: metagenome

text: Homo sapiens

text: 16S rDNA saliva study

text: Homo sapiens

text: Pyrosequencing

text: Whole unstimulated saliva samples

characteristic: title


K9 (healthy saliva sample)

characteristic: ENA-CHECKLIST

text: public

text: K9 (healthy saliva sample)

text: human

text: saliva

text: oral

text: DNA isolated from saliva

text: Norway

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: human-oral

characteristic: humam oral environmental package

text: metagenome

characteristic: investigation type

text: Homo sapiens

text: 16S rDNA saliva study

characteristic: project name

text: Homo sapiens

text: Pyrosequencing

characteristic: sequencing method

text: Whole unstimulated saliva samples


K10 (healthy saliva sample)

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: K10 (healthy saliva sample)

characteristic: Submitter Id

text: human

text: saliva

text: oral

text: DNA isolated from saliva

text: Norway

text: human-oral

text: metagenome

text: Homo sapiens

characteristic: organism

text: 16S rDNA saliva study

text: Homo sapiens

text: Pyrosequencing

text: Whole unstimulated saliva samples


K12 (healthy saliva sample)

characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: INSDC status

text: K12 (healthy saliva sample)

characteristic: collection date

text: human

characteristic: common name

text: saliva

characteristic: environment (biome)

text: oral

characteristic: environment (feature)

text: DNA isolated from saliva

characteristic: environment (material)

text: Norway

text: human-oral

text: metagenome

text: Homo sapiens

text: 16S rDNA saliva study

text: Homo sapiens

text: Pyrosequencing

text: Whole unstimulated saliva samples

characteristic: title


S1 (pSS saliva sample)

characteristic: ENA-CHECKLIST

text: public

text: S1 (pSS saliva sample)

text: human

text: saliva

text: oral

text: DNA isolated from saliva

text: Norway

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: human-oral

characteristic: humam oral environmental package

text: metagenome

characteristic: investigation type

text: Homo sapiens

text: 16S rDNA saliva study

characteristic: project name

text: Homo sapiens

text: Pyrosequencing

characteristic: sequencing method

text: Whole unstimulated saliva samples


S3 (pSS saliva sample)

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: S3 (pSS saliva sample)

characteristic: Submitter Id

text: human

text: saliva

text: oral

text: DNA isolated from saliva

text: Norway

text: human-oral

text: metagenome

text: Homo sapiens

characteristic: organism

text: 16S rDNA saliva study

text: Homo sapiens

text: Pyrosequencing

text: Whole unstimulated saliva samples


S4 (pSS saliva sample)

characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: INSDC status

text: S4 (pSS saliva sample)

characteristic: collection date

text: human

characteristic: common name

text: saliva

characteristic: environment (biome)

text: oral

characteristic: environment (feature)

text: DNA isolated from saliva

characteristic: environment (material)

text: Norway

text: human-oral

text: metagenome

text: Homo sapiens

text: 16S rDNA saliva study

text: Homo sapiens

text: Pyrosequencing

text: Whole unstimulated saliva samples

characteristic: title


S5 (pSS saliva sample)

characteristic: ENA-CHECKLIST

text: public

text: S5 (pSS saliva sample)

text: human

text: saliva

text: oral

text: DNA isolated from saliva

text: Norway

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: human-oral

characteristic: humam oral environmental package

text: metagenome

characteristic: investigation type

text: Homo sapiens

text: 16S rDNA saliva study

characteristic: project name

text: Homo sapiens

text: Pyrosequencing

characteristic: sequencing method

text: Whole unstimulated saliva samples


S6 (pSS saliva sample)

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: S6 (pSS saliva sample)

characteristic: Submitter Id

text: human

text: saliva

text: oral

text: DNA isolated from saliva

text: Norway

text: human-oral

text: metagenome

text: Homo sapiens

characteristic: organism

text: 16S rDNA saliva study

text: Homo sapiens

text: Pyrosequencing

text: Whole unstimulated saliva samples


S7 (pSS saliva sample)

characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: INSDC status

text: S7 (pSS saliva sample)

characteristic: collection date

text: human

characteristic: common name

text: saliva

characteristic: environment (biome)

text: oral

characteristic: environment (feature)

text: DNA isolated from saliva

characteristic: environment (material)

text: Norway

text: human-oral

text: metagenome

text: Homo sapiens

text: 16S rDNA saliva study

text: Homo sapiens

text: Pyrosequencing

text: Whole unstimulated saliva samples

characteristic: title


S8 (pSS saliva sample)

characteristic: ENA-CHECKLIST

text: public

text: S8 (pSS saliva sample)

text: human

text: saliva

text: oral

text: DNA isolated from saliva

text: Norway

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: human-oral

characteristic: humam oral environmental package

text: metagenome

characteristic: investigation type

text: Homo sapiens

text: 16S rDNA saliva study

characteristic: project name

text: Homo sapiens

text: Pyrosequencing

characteristic: sequencing method

text: Whole unstimulated saliva samples


S9 (pSS saliva sample)

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: S9 (pSS saliva sample)

characteristic: Submitter Id

text: human

text: saliva

text: oral

text: DNA isolated from saliva

text: Norway

text: human-oral

text: metagenome

text: Homo sapiens

characteristic: organism

text: 16S rDNA saliva study

text: Homo sapiens

text: Pyrosequencing

text: Whole unstimulated saliva samples


S11 (pSS saliva sample)

characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: INSDC status

text: S11 (pSS saliva sample)

characteristic: collection date

text: human

characteristic: common name

text: saliva

characteristic: environment (biome)

text: oral

characteristic: environment (feature)

text: DNA isolated from saliva

characteristic: environment (material)

text: Norway

text: human-oral

text: metagenome

text: Homo sapiens

text: 16S rDNA saliva study

text: Homo sapiens

text: Pyrosequencing

text: Whole unstimulated saliva samples

characteristic: title


characteristic: ENA-CHECKLIST

text: INRA (INRA Rennes)

text: public

tag: Namespace:INRA (INRA Rennes)

text: bovine metagenome

text: foremilk samples and swab samples of the teat canal

characteristic: description

text: bovine metagenome

text: bovine metagenome

text: bovine teat microbiota sample


E-MTAB-4368:Sample 1

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-4368:Sample 1

tag: Namespace:Laboratory of Molecular Medicine and Genomics Department of Medicine and Surgery University of Salerno

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: human

text: Protocols: RNA was extracted from cells expressing or not ER using the standard RNA extraction method with TRIzol (Life Technologies) After Ago2 knock-down in C-TAP-ER and MCF7 cells starved for 5 days, nascent RNA was extracted from each sample following the procedure developed by Khodor et al.28. Nascent RNA isolation was also conducted on non-transfected and transfected with pLKO.1-puro Non-Target shRNA Control Plasmid DNA (Sigma-Aldrich) cells as control. TRIzol reagent (Life Techonolgies) was added, an incubation at 65C was performed to dissolve DNA-Histone-Pol II-RNA pellets28 and RNA was extracted following the manufacturers protocol. For sequencing, indexed libraries were prepared using 1 g of Nascent RNA as starting material, with TruSeq Stranded Total RNA Sample Prep Kit (Illumina Inc.)

text: expression of estrogen receptor fused to C terminus tandem affinity purification tag

characteristic: genotype

text: Homo sapiens

text: Homo sapiens

text: expression of estrogen receptor

characteristic: phenotype

text: Homo sapiens

text: Sample 1


E-MTAB-4368:Sample 10

text: Laboratory of Molecular Medicine and Genomics Department of Medicine and Surgery University of Salerno

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: E-MTAB-4368:Sample 10

characteristic: Submitter Id

text: ArrayExpress

text: human

text: Protocols: C-TAP-ER and MCF7 control cells were starved for 5 days and then Ago2 knock-down was performed using a combination of three pLKO.1 plasmid vectors expressing shRNAs (Sigma Aldrich: TRCN0000007864; TRCN0000007867; TRCN0000011203) targeting Ago2 transcript (GenBankTM accession number NM_012154 ) in different regions. Ago2 silencing was conducted by co-transfecting C-TAP-ER and control cells with shRNA vectors, using Lipofectamine 2000 (Life Technologies), for 48 hours. The transfection medium was replaced with fresh culturing medium 6 h after treatment. Non-transfected and transfected cells with pLKO.1-puro Non-Target shRNA Control Plasmid DNA (Sigma-Aldrich) were used as control. Western blotting and qPCR were performed to verify the depletion of our target protein and mRNA, respectively. cDNA synthesis and qPCR Using 1 g of RNA as starting material, retro-transcription was performed employing AffinityScript cDNA Synthesis Kit (Agilent). qPCRs was performed in duplicate on Stratagene Mx3005PTM instrument (Agilent Technologies) using SensiFASTTM SYBR Lo-ROX kit (Bioline). 40 cycles of amplification were performed. Primers used for qPCR are: for GAPDH: Forward primer: GAGAAGGCTGGGGCTCATTT Reverse primer: GCAGGAGGCATTGCTGATGA for AGO2: Forward primer: AAGTCCTTCACAGAGCAGCT Reverse primer: CTGCTCCTGTCCGACTTGTA After Ago2 knock-down in C-TAP-ER and MCF7 cells starved for 5 days, nascent RNA was extracted from each sample following the procedure developed by Khodor et al.28. Nascent RNA isolation was also conducted on non-transfected and transfected with pLKO.1-puro Non-Target shRNA Control Plasmid DNA (Sigma-Aldrich) cells as control. TRIzol reagent (Life Techonolgies) was added, an incubation at 65C was performed to dissolve DNA-Histone-Pol II-RNA pellets28 and RNA was extracted following the manufacturers protocol. For sequencing, indexed libraries were prepared using 1 g of Nascent RNA as starting material, with TruSeq Stranded Total RNA Sample Prep Kit (Illumina Inc.)

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: no expression of estrogen receptor / shAgo2

text: shRNA targeting Ago2

characteristic: rna interference

text: Homo sapiens

text: Sample 10


E-MTAB-4368:Sample 2

characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: INSDC status

text: E-MTAB-4368:Sample 2

text: ArrayExpress

text: human

characteristic: common name

text: Homo sapiens

text: Homo sapiens

text: expression of estrogen receptor

text: Homo sapiens

text: Sample 2


E-MTAB-4368:Sample 3

characteristic: ENA-CHECKLIST

text: public

text: E-MTAB-4368:Sample 3

text: ArrayExpress

text: human

characteristic: description

text: Homo sapiens

text: Homo sapiens

text: expression of estrogen receptor / shAgo2

text: shRNA targeting Ago2

text: Homo sapiens

text: Sample 3

characteristic: title


E-MTAB-4368:Sample 4

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-4368:Sample 4

text: ArrayExpress

characteristic: broker name

text: human

text: Homo sapiens

text: Homo sapiens

text: shRNA targeting Ago2

text: Homo sapiens

text: Sample 4


E-MTAB-4368:Sample 7

text: public

text: E-MTAB-4368:Sample 7

text: ArrayExpress

characteristic: cell line

text: human

text: Homo sapiens

text: Homo sapiens

text: no expression of estrogen receptor

characteristic: phenotype

text: Homo sapiens

text: Sample 7


E-MTAB-4368:Sample 8

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-4368:Sample 8

text: ArrayExpress

text: human

text: Homo sapiens

text: Homo sapiens

text: no expression of estrogen receptor

text: Homo sapiens

text: Sample 8


E-MTAB-4368:Sample 9

characteristic: INSDC last update

text: public

characteristic: SRA accession

text: E-MTAB-4368:Sample 9

text: ArrayExpress

text: human

text: Homo sapiens

text: Homo sapiens

text: shRNA targeting Ago2

text: Homo sapiens

text: Sample 9


WT alpha-factor input 39

text: WEIZMANN INSTITUE OF SCIENCE

text: public

text: WT alpha-factor input 39

tag: Namespace:WEIZMANN INSTITUE OF SCIENCE

characteristic: Submitter Id

text: input

characteristic: antibody

text: baker's yeast

text: Saccharomyces cerevisiae

characteristic: organism

text: Saccharomyces cerevisiae

text: MATa, his3?1, leu2?0, met15?0, ura3?0

characteristic: strain

characteristic: time

text: WT (DNA content experiment)

characteristic: time course repeat

text: WT alpha-factor input 39


WT alpha-factor input 36

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: WEIZMANN INSTITUE OF SCIENCE

text: public

characteristic: INSDC status

text: WT alpha-factor input 36

text: input

text: baker's yeast

characteristic: common name

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: MATa, his3?1, leu2?0, met15?0, ura3?0

text: WT (DNA content experiment)

text: WT alpha-factor input 36


WT alpha-factor input 33

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: WEIZMANN INSTITUE OF SCIENCE

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text: WT alpha-factor input 33

text: input

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: MATa, his3?1, leu2?0, met15?0, ura3?0

text: WT (DNA content experiment)

text: WT alpha-factor input 33

characteristic: title


WT alpha-factor input 30

text: WEIZMANN INSTITUE OF SCIENCE

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text: input

text: baker's yeast

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text: MATa, his3?1, leu2?0, met15?0, ura3?0

text: WT (DNA content experiment)

text: WT alpha-factor input 30


WT alpha-factor input 27

text: WEIZMANN INSTITUE OF SCIENCE

characteristic: INSDC first public

text: public

text: WT alpha-factor input 27

text: input

text: baker's yeast

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text: MATa, his3?1, leu2?0, met15?0, ura3?0

text: WT (DNA content experiment)

text: WT alpha-factor input 27


WT alpha-factor input 24

text: WEIZMANN INSTITUE OF SCIENCE

characteristic: INSDC last update

text: public

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text: input

text: baker's yeast

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text: WT alpha-factor input 24


WT alpha-factor input 21

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characteristic: SRA accession

text: WT alpha-factor input 21

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text: baker's yeast

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WT alpha-factor input 18

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WT alpha-factor input 15

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characteristic: antibody

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text: WT (DNA content experiment)

text: WT alpha-factor input 15


WT alpha-factor input 12

text: WEIZMANN INSTITUE OF SCIENCE

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text: baker's yeast

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WT alpha-factor input 9

text: WEIZMANN INSTITUE OF SCIENCE

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text: baker's yeast

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text: WT (DNA content experiment)

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WT alpha-factor input sync

text: WEIZMANN INSTITUE OF SCIENCE

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text: baker's yeast

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characteristic: time

text: WT (DNA content experiment)

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WT alpha-factor H3K56ac 39

text: WEIZMANN INSTITUE OF SCIENCE

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characteristic: time course repeat

text: WT alpha-factor H3K56ac 39


WT alpha-factor H3K56ac 36

characteristic: ENA-CHECKLIST

text: WEIZMANN INSTITUE OF SCIENCE

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text: baker's yeast

text: Saccharomyces cerevisiae

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text: MATa, his3?1, leu2?0, met15?0, ura3?0

text: WT (DNA content experiment)

text: WT alpha-factor H3K56ac 36


WT alpha-factor H3K56ac 33

characteristic: ENA-FIRST-PUBLIC

text: WEIZMANN INSTITUE OF SCIENCE

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text: baker's yeast

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text: WT (DNA content experiment)

text: WT alpha-factor H3K56ac 33


WT alpha-factor H3K56ac 30

text: WEIZMANN INSTITUE OF SCIENCE

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characteristic: INSDC status

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text: WT (DNA content experiment)

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WT alpha-factor input 39 run repeat

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WT alpha-factor input 24 run repeat

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