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characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

text: weeks

characteristic: age unit

characteristic: altitude

characteristic: anonymized name

text: FALSE

text: UBERON:gastro-intestinal system

characteristic: body habitat

text: UBERON:digestive tract lumen

characteristic: body product

text: UBERON:proximal colon

characteristic: body site

text: Missing: Not provided

text: pig gut metagenome

characteristic: common name

text: USA

characteristic: country

characteristic: depth

text: proximal colon digesta .8

characteristic: description

text: TRUE

text: urban biome

text: animal-associated habitat

text: excreta

text: host-associated

text: excreta

text: USA:CO:Fort Collins

text: TRUE

text: pig

text: Sus scrofula

characteristic: host scientific name

text: pig gut metagenome

characteristic: organism

text: U Penn

text: FALSE

text: FALSE

text: completed

text: excreta

text: pig gut metagenome

text: male

text: Final

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: weeks

text: FALSE

text: UBERON:gastro-intestinal system

text: UBERON:digestive tract lumen

text: UBERON:proximal colon

text: Missing: Not provided

text: pig gut metagenome

text: USA

text: proximal colon digesta .9

text: TRUE

characteristic: elevation

text: urban biome

characteristic: env biome

text: animal-associated habitat

characteristic: env feature

text: excreta

characteristic: env matter

text: host-associated

characteristic: env package

text: excreta

text: USA:CO:Fort Collins

characteristic: geo loc name

text: TRUE

text: pig

characteristic: host common name

text: Sus scrofula

characteristic: host subject id

characteristic: host taxid

characteristic: latitude

characteristic: longitude

text: pig gut metagenome

text: U Penn

text: FALSE

text: FALSE

characteristic: public

text: completed

text: excreta

characteristic: sample type

text: pig gut metagenome

text: male

characteristic: sex

text: Final

characteristic: timepoint

characteristic: treatment


BAZMOX

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: BAZMOX

tag: Namespace:MAX PLANCK INSTITUTE FOR MARINE MICROBIOLOGY

characteristic: Submitter Id

characteristic: collection date

text: Draft genome assembly of methane-oxidizer endosymbiont of Bathymodiolus azoricus

characteristic: description

text: Hydrothermal vent

characteristic: environment (biome)

text: ocean

characteristic: environment (feature)

text: water

characteristic: environment (material)

text: Atlantic Ocean

characteristic: geographic location (country and/or sea)

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: host-associated

characteristic: host-associated environmental package

text: metagenome

characteristic: investigation type

text: methanotrophic endosymbiont of Bathymodiolus azoricus (Menez Gwen)

characteristic: organism

text: Draft genome of the methane-oxidizing symbiont of Bathymodiolus azoricus (Menez Gwen)

characteristic: project name

characteristic: sequencing method

text: Draft genome of methane-oxidizer endosymbiont of Bathymodiolus azoricus

characteristic: title


METH

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: MAX PLANCK INSTITUTE FOR MARINE MICROBIOLOGY

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: METH

text: The methanotrophic symbionts were labeled with 16S rRNA CARD-FISH to sort them by FACS and then specifically only sequence the MOX with MDA

text: Hydrothermal vent

text: ocean

text: water

text: Atlantic Ocean

text: host-associated

text: metagenome

text: methanotrophic bacterial endosymbiont of Bathymodiolus sp.

text: Bathybiome

text: Methanotroph symbiont of Bathymodiolus sp. from Lilliput and Foggy Corner


text: OXFORD

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: Rotavirus A

text: Rotavirus A

text: Rotavirus


text: DTU-CFB

text: public

characteristic: collection date

text: fecal sample

characteristic: environment (biome)

text: fecal sample

characteristic: environment (feature)

text: fecal sample

characteristic: environment (material)

text: Netherlands

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: human-gut

characteristic: human gut environmental package

text: metagenome

text: human gut metagenome

text: poreFUME

text: human gut metagenome

text: MinION nanopore

text: poreFUME sample


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: WDRC KAUST

text: public

tag: Namespace:WDRC KAUST

text: MAP inoculum

characteristic: description

text: Reactor

text: Anaerobic

text: Inoculum

text: USA

text: metagenome

characteristic: investigation type

text: miscellaneous natural or artificial environment

characteristic: miscellaneous environmental package

text: metagenome

characteristic: organism

characteristic: project name

text: metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: MAP inoculum

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: WDRC KAUST

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: H inoculum

text: Reactor

text: Anaerobic

text: Inoculum

text: USA

text: metagenome

text: metagenome

text: metagenome

text: Illumina MiSeq

text: H inoculum


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: WDRC KAUST

text: public

text: M1 acclimation reactor

text: Reactor

characteristic: environment (biome)

text: Anaerobic

characteristic: environment (feature)

text: Fluid

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

text: metagenome

text: metagenome

text: Illumina MiSeq

text: M1 acclimation reactor


characteristic: ENA-LAST-UPDATE

text: WDRC KAUST

text: public

text: M2 acclimation reactor

characteristic: description

text: Reactor

text: Anaerobic

text: Fluid

text: USA

text: metagenome

characteristic: investigation type

characteristic: miscellaneous environmental package

text: metagenome

characteristic: organism

characteristic: project name

text: metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: M2 acclimation reactor

characteristic: title


characteristic: External Id

text: WDRC KAUST

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: MAP1 acclimation reactor

text: Reactor

text: Anaerobic

text: Fluid

text: USA

text: metagenome

text: metagenome

text: metagenome

text: Illumina MiSeq

text: MAP1 acclimation reactor


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: WDRC KAUST

text: public

text: MAP2 acclimation reactor

text: Reactor

characteristic: environment (biome)

text: Anaerobic

characteristic: environment (feature)

text: Fluid

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

text: metagenome

text: metagenome

text: Illumina MiSeq

text: MAP2 acclimation reactor


characteristic: ENA-LAST-UPDATE

text: WDRC KAUST

text: public

text: H1 acclimation reactor

characteristic: description

text: Reactor

text: Anaerobic

text: Fluid

text: USA

text: metagenome

characteristic: investigation type

characteristic: miscellaneous environmental package

text: metagenome

characteristic: organism

characteristic: project name

text: metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: H1 acclimation reactor

characteristic: title


characteristic: External Id

text: WDRC KAUST

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: H2 acclimation reactor

text: Reactor

text: Anaerobic

text: Fluid

text: USA

text: metagenome

text: metagenome

text: metagenome

text: Illumina MiSeq

text: H2 acclimation reactor


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: WDRC KAUST

text: public

text: M1 MEC, GAC

text: Reactor

characteristic: environment (biome)

text: Anaerobic

characteristic: environment (feature)

text: GAC

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

text: metagenome

text: metagenome

text: Illumina MiSeq

text: M1 MEC, GAC


characteristic: ENA-LAST-UPDATE

text: WDRC KAUST

text: public

text: M1 MEC, brush

characteristic: description

text: Reactor

text: Anaerobic

text: Brush

text: USA

text: metagenome

characteristic: investigation type

characteristic: miscellaneous environmental package

text: metagenome

characteristic: organism

characteristic: project name

text: metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: M1 MEC, brush

characteristic: title


characteristic: External Id

text: WDRC KAUST

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: M2 MEC, GAC

text: Reactor

text: Anaerobic

text: GAC

text: USA

text: metagenome

text: metagenome

text: metagenome

text: Illumina MiSeq

text: M2 MEC, GAC


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: WDRC KAUST

text: public

text: M2 MEC, brush

text: Reactor

characteristic: environment (biome)

text: Anaerobic

characteristic: environment (feature)

text: Brush

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

text: metagenome

text: metagenome

text: Illumina MiSeq

text: M2 MEC, brush


characteristic: ENA-LAST-UPDATE

text: WDRC KAUST

text: public

text: MAP1 MEC, GAC

characteristic: description

text: Reactor

text: Anaerobic

text: GAC

text: USA

text: metagenome

characteristic: investigation type

characteristic: miscellaneous environmental package

text: metagenome

characteristic: organism

characteristic: project name

text: metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: MAP1 MEC, GAC

characteristic: title


characteristic: External Id

text: WDRC KAUST

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: MAP1 MEC, brush

text: Reactor

text: Anaerobic

text: Brush

text: USA

text: metagenome

text: metagenome

text: metagenome

text: Illumina MiSeq

text: MAP1 MEC, brush


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: WDRC KAUST

text: public

text: MAP2 MEC, GAC

text: Reactor

characteristic: environment (biome)

text: Anaerobic

characteristic: environment (feature)

text: GAC

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

text: metagenome

text: metagenome

text: Illumina MiSeq

text: MAP2 MEC, GAC


characteristic: ENA-LAST-UPDATE

text: WDRC KAUST

text: public

text: MAP2 MEC, brush

characteristic: description

text: Reactor

text: Anaerobic

text: Brush

text: USA

text: metagenome

characteristic: investigation type

characteristic: miscellaneous environmental package

text: metagenome

characteristic: organism

characteristic: project name

text: metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: MAP2 MEC, brush

characteristic: title


characteristic: External Id

text: WDRC KAUST

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: H1 MEC, GAC

text: Reactor

text: Anaerobic

text: GAC

text: USA

text: metagenome

text: metagenome

text: metagenome

text: Illumina MiSeq

text: H1 MEC, GAC


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: WDRC KAUST

text: public

text: H1 MEC, brush

text: Reactor

characteristic: environment (biome)

text: Anaerobic

characteristic: environment (feature)

text: Brush

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

text: metagenome

text: metagenome

text: Illumina MiSeq

text: H1 MEC, brush


characteristic: ENA-LAST-UPDATE

text: WDRC KAUST

text: public

text: H2 MEC, GAC

characteristic: description

text: Reactor

text: Anaerobic

text: GAC

text: USA

text: metagenome

characteristic: investigation type

characteristic: miscellaneous environmental package

text: metagenome

characteristic: organism

characteristic: project name

text: metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: H2 MEC, GAC

characteristic: title


characteristic: External Id

text: WDRC KAUST

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: H2 MEC, brush

text: Reactor

text: Anaerobic

text: Brush

text: USA

text: metagenome

text: metagenome

text: metagenome

text: Illumina MiSeq

text: H2 MEC, brush


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: WDRC KAUST

text: public

text: GAC+bog1 MEC, GAC

text: Reactor

characteristic: environment (biome)

text: Anaerobic

characteristic: environment (feature)

text: GAC

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

text: metagenome

text: metagenome

text: Illumina MiSeq

text: GAC+bog1 MEC, GAC


characteristic: ENA-LAST-UPDATE

text: WDRC KAUST

text: public

text: GAC+bog1 MEC, brush

characteristic: description

text: Reactor

text: Anaerobic

text: Brush

text: USA

text: metagenome

characteristic: investigation type

characteristic: miscellaneous environmental package

text: metagenome

characteristic: organism

characteristic: project name

text: metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: GAC+bog1 MEC, brush

characteristic: title


characteristic: External Id

text: WDRC KAUST

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: GAC+bog2 MEC, GAC

text: Reactor

text: Anaerobic

text: GAC

text: USA

text: metagenome

text: metagenome

text: metagenome

text: Illumina MiSeq

text: GAC+bog2 MEC, GAC


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: WDRC KAUST

text: public

text: GAC+bog2 MEC, brush

text: Reactor

characteristic: environment (biome)

text: Anaerobic

characteristic: environment (feature)

text: Brush

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

text: metagenome

text: metagenome

text: Illumina MiSeq

text: GAC+bog2 MEC, brush


E-MTAB-4878:Sample 1

characteristic: ENA-LAST-UPDATE

text: University of Liverpool

text: public

text: E-MTAB-4878:Sample 1

tag: Namespace:University of Liverpool

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

text: Protocols: Skin removed from hind legs and stifle joints collected. Approximately 4mm each side of the joint was collected with no muscle included. destabilization of the medial meniscus (DMM) model of traumatic osteoarthritis young normal donor 100ng of small RNA was added in a volume of 6ul together with 1ul of 3’SR adapter (diluted 1+2). This was heated at 70 degrees for 2minutes and cooled on ice. Ligation buffer and enzyme were added and the reaction incubated for 1 hour at 25 degrees. 2ul of Tobacco Acid Pyrophosphatase (TAP)(Epicentre) 5u/ul was added to each reaction and incubated at 37 degrees for 1 hour. Tobacco Acid Pyrophosphatase (TAP) hydrolyzes the phosphoric acid anhydride bonds in the triphosphate bridge of the cap structure found in most eukaryotic mRNA, releasing the cap nucleoside and generating a 5 ́-monophosphorylated terminus on the RNA molecule. This is inactivated in the subsequent step which heats the samples to 75 degrees for 5 minutes The samples were hybridized to the Reverse Transcription Primer at a dilution of 1+2 from the supplied stock of primer. This step is important to prevent adaptor-dimer formation. The SR RT Primer hybridizes to the excess of the 3 ́ SR Adaptor (that remains free after the 3 ́ ligation reaction) and transforms the single stranded DNA adaptor into a double-stranded DNA molecule. dsDNAs are not substrates for ligation mediated by T4 RNA Ligase 1 and therefore do not ligate to the 5 ́ SR Adaptor in the subsequent ligation step. Following this 5”ligation for 1 hour at 25 degrees, a reverse transcription was carried out at 50 degrees for 1 hour using ProtoScript II Reverse Transcriptase. The samples were then amplified for 15 cycles and incorporating a barcode as part of the priming process. The samples were mixed into 3 pools,(mixing across groups) and size selected on a Sage Pippin cassette 2% DF marker E using a size range 120-300bp. The size selected material was purified with Ampure beads(1:1) (Agencourt). The pools were checked by Qubit analysis and Bioanalyser High Sensitivity chip.

characteristic: description

text: Young

characteristic: developmental stage

text: Mus musculus

characteristic: organism

text: Mus musculus

text: Joint

characteristic: organism part

text: Mus musculus

characteristic: strain

text: Sample 1

characteristic: title


E-MTAB-4878:Sample 10

characteristic: ENA-CHECKLIST

characteristic: External Id

text: University of Liverpool

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-4878:Sample 10

characteristic: Submitter Id

text: ArrayExpress

text: house mouse

text: Protocols: Skin removed from hind legs and stifle joints collected. Approximately 4mm each side of the joint was collected with no muscle included. old normal donor 100ng of small RNA was added in a volume of 6ul together with 1ul of 3’SR adapter (diluted 1+2). This was heated at 70 degrees for 2minutes and cooled on ice. Ligation buffer and enzyme were added and the reaction incubated for 1 hour at 25 degrees. 2ul of Tobacco Acid Pyrophosphatase (TAP)(Epicentre) 5u/ul was added to each reaction and incubated at 37 degrees for 1 hour. Tobacco Acid Pyrophosphatase (TAP) hydrolyzes the phosphoric acid anhydride bonds in the triphosphate bridge of the cap structure found in most eukaryotic mRNA, releasing the cap nucleoside and generating a 5 ́-monophosphorylated terminus on the RNA molecule. This is inactivated in the subsequent step which heats the samples to 75 degrees for 5 minutes The samples were hybridized to the Reverse Transcription Primer at a dilution of 1+2 from the supplied stock of primer. This step is important to prevent adaptor-dimer formation. The SR RT Primer hybridizes to the excess of the 3 ́ SR Adaptor (that remains free after the 3 ́ ligation reaction) and transforms the single stranded DNA adaptor into a double-stranded DNA molecule. dsDNAs are not substrates for ligation mediated by T4 RNA Ligase 1 and therefore do not ligate to the 5 ́ SR Adaptor in the subsequent ligation step. Following this 5”ligation for 1 hour at 25 degrees, a reverse transcription was carried out at 50 degrees for 1 hour using ProtoScript II Reverse Transcriptase. The samples were then amplified for 15 cycles and incorporating a barcode as part of the priming process. The samples were mixed into 3 pools,(mixing across groups) and size selected on a Sage Pippin cassette 2% DF marker E using a size range 120-300bp. The size selected material was purified with Ampure beads(1:1) (Agencourt). The pools were checked by Qubit analysis and Bioanalyser High Sensitivity chip.

text: Old

text: Mus musculus

text: Mus musculus

text: Joint

text: Mus musculus

text: Sample 10


E-MTAB-4878:Sample 11

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: University of Liverpool

text: public

text: E-MTAB-4878:Sample 11

text: ArrayExpress

text: house mouse

text: Old

text: Mus musculus

text: Mus musculus

text: Joint

text: Mus musculus

text: Sample 11


E-MTAB-4878:Sample 12

text: University of Liverpool

text: public

text: E-MTAB-4878:Sample 12

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

text: Old

text: Mus musculus

text: Mus musculus

text: Joint

text: Mus musculus

text: Sample 12


E-MTAB-4878:Sample 13

text: University of Liverpool

text: public

text: E-MTAB-4878:Sample 13

text: ArrayExpress

text: house mouse

text: Protocols: Serum collected from heart following sacrifice. young normal donor 100ng of small RNA was added in a volume of 6ul together with 1ul of 3’SR adapter (diluted 1+2). This was heated at 70 degrees for 2minutes and cooled on ice. Ligation buffer and enzyme were added and the reaction incubated for 1 hour at 25 degrees. 2ul of Tobacco Acid Pyrophosphatase (TAP)(Epicentre) 5u/ul was added to each reaction and incubated at 37 degrees for 1 hour. Tobacco Acid Pyrophosphatase (TAP) hydrolyzes the phosphoric acid anhydride bonds in the triphosphate bridge of the cap structure found in most eukaryotic mRNA, releasing the cap nucleoside and generating a 5 ́-monophosphorylated terminus on the RNA molecule. This is inactivated in the subsequent step which heats the samples to 75 degrees for 5 minutes The samples were hybridized to the Reverse Transcription Primer at a dilution of 1+2 from the supplied stock of primer. This step is important to prevent adaptor-dimer formation. The SR RT Primer hybridizes to the excess of the 3 ́ SR Adaptor (that remains free after the 3 ́ ligation reaction) and transforms the single stranded DNA adaptor into a double-stranded DNA molecule. dsDNAs are not substrates for ligation mediated by T4 RNA Ligase 1 and therefore do not ligate to the 5 ́ SR Adaptor in the subsequent ligation step. Following this 5”ligation for 1 hour at 25 degrees, a reverse transcription was carried out at 50 degrees for 1 hour using ProtoScript II Reverse Transcriptase. The samples were then amplified for 15 cycles and incorporating a barcode as part of the priming process. The samples were mixed into 3 pools,(mixing across groups) and size selected on a Sage Pippin cassette 2% DF marker E using a size range 120-300bp. The size selected material was purified with Ampure beads(1:1) (Agencourt). The pools were checked by Qubit analysis and Bioanalyser High Sensitivity chip.

characteristic: description

text: Young

characteristic: developmental stage

text: Mus musculus

text: Mus musculus

text: Serum

text: Mus musculus

text: Sample 13


E-MTAB-4878:Sample 14

text: University of Liverpool

text: public

text: E-MTAB-4878:Sample 14

text: ArrayExpress

text: house mouse

text: Young

text: Mus musculus

characteristic: organism

text: Mus musculus

text: Serum

characteristic: organism part

text: Mus musculus

text: Sample 14


E-MTAB-4878:Sample 15

text: University of Liverpool

text: public

text: E-MTAB-4878:Sample 15

text: ArrayExpress

text: house mouse

text: Young

text: Mus musculus

text: Mus musculus

text: Serum

text: Mus musculus

characteristic: strain

text: Sample 15

characteristic: title


E-MTAB-4878:Sample 16

characteristic: ENA-CHECKLIST

characteristic: External Id

text: University of Liverpool

text: public

text: E-MTAB-4878:Sample 16

text: ArrayExpress

text: house mouse

text: Young

text: Mus musculus

text: Mus musculus

text: Serum

text: Mus musculus

text: Sample 16


E-MTAB-4878:Sample 17

text: University of Liverpool

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-4878:Sample 17

text: ArrayExpress

text: house mouse

text: Young

text: Mus musculus

text: Mus musculus

text: Serum

text: Mus musculus

text: Sample 17


E-MTAB-4878:Sample 18

text: University of Liverpool

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-4878:Sample 18

text: ArrayExpress

text: house mouse

text: Young

text: Mus musculus

text: Mus musculus

text: Serum

text: Mus musculus

text: Sample 18


E-MTAB-4878:Sample 19

text: University of Liverpool

text: public

characteristic: SRA accession

text: E-MTAB-4878:Sample 19

characteristic: Submitter Id

text: ArrayExpress

text: house mouse

text: Protocols: Serum collected from heart following sacrifice. old normal donor 100ng of small RNA was added in a volume of 6ul together with 1ul of 3’SR adapter (diluted 1+2). This was heated at 70 degrees for 2minutes and cooled on ice. Ligation buffer and enzyme were added and the reaction incubated for 1 hour at 25 degrees. 2ul of Tobacco Acid Pyrophosphatase (TAP)(Epicentre) 5u/ul was added to each reaction and incubated at 37 degrees for 1 hour. Tobacco Acid Pyrophosphatase (TAP) hydrolyzes the phosphoric acid anhydride bonds in the triphosphate bridge of the cap structure found in most eukaryotic mRNA, releasing the cap nucleoside and generating a 5 ́-monophosphorylated terminus on the RNA molecule. This is inactivated in the subsequent step which heats the samples to 75 degrees for 5 minutes The samples were hybridized to the Reverse Transcription Primer at a dilution of 1+2 from the supplied stock of primer. This step is important to prevent adaptor-dimer formation. The SR RT Primer hybridizes to the excess of the 3 ́ SR Adaptor (that remains free after the 3 ́ ligation reaction) and transforms the single stranded DNA adaptor into a double-stranded DNA molecule. dsDNAs are not substrates for ligation mediated by T4 RNA Ligase 1 and therefore do not ligate to the 5 ́ SR Adaptor in the subsequent ligation step. Following this 5”ligation for 1 hour at 25 degrees, a reverse transcription was carried out at 50 degrees for 1 hour using ProtoScript II Reverse Transcriptase. The samples were then amplified for 15 cycles and incorporating a barcode as part of the priming process. The samples were mixed into 3 pools,(mixing across groups) and size selected on a Sage Pippin cassette 2% DF marker E using a size range 120-300bp. The size selected material was purified with Ampure beads(1:1) (Agencourt). The pools were checked by Qubit analysis and Bioanalyser High Sensitivity chip.

text: Old

text: Mus musculus

text: Mus musculus

text: Serum

text: Mus musculus

text: Sample 19


E-MTAB-4878:Sample 2

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: University of Liverpool

text: public

text: E-MTAB-4878:Sample 2

text: ArrayExpress

text: house mouse

text: Young

text: Mus musculus

text: Mus musculus

text: Joint

text: Mus musculus

text: Sample 2


E-MTAB-4878:Sample 20

text: University of Liverpool

text: public

text: E-MTAB-4878:Sample 20

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

text: Old

text: Mus musculus

text: Mus musculus

text: Serum

text: Mus musculus

text: Sample 20


E-MTAB-4878:Sample 21

text: University of Liverpool

text: public

text: E-MTAB-4878:Sample 21

text: ArrayExpress

text: house mouse

characteristic: description

text: Old

characteristic: developmental stage

text: Mus musculus

text: Mus musculus

text: Serum

text: Mus musculus

text: Sample 21


E-MTAB-4878:Sample 22

text: University of Liverpool

text: public

text: E-MTAB-4878:Sample 22

text: ArrayExpress

text: house mouse

text: Old

text: Mus musculus

characteristic: organism

text: Mus musculus

text: Serum

characteristic: organism part

text: Mus musculus

text: Sample 22


E-MTAB-4878:Sample 23

text: University of Liverpool

text: public

text: E-MTAB-4878:Sample 23

text: ArrayExpress

text: house mouse

text: Protocols: Serum collected from heart following sacrifice. Skin removed from hind legs and stifle joints collected. Approximately 4mm each side of the joint was collected with no muscle included. old normal donor 100ng of small RNA was added in a volume of 6ul together with 1ul of 3’SR adapter (diluted 1+2). This was heated at 70 degrees for 2minutes and cooled on ice. Ligation buffer and enzyme were added and the reaction incubated for 1 hour at 25 degrees. 2ul of Tobacco Acid Pyrophosphatase (TAP)(Epicentre) 5u/ul was added to each reaction and incubated at 37 degrees for 1 hour. Tobacco Acid Pyrophosphatase (TAP) hydrolyzes the phosphoric acid anhydride bonds in the triphosphate bridge of the cap structure found in most eukaryotic mRNA, releasing the cap nucleoside and generating a 5 ́-monophosphorylated terminus on the RNA molecule. This is inactivated in the subsequent step which heats the samples to 75 degrees for 5 minutes The samples were hybridized to the Reverse Transcription Primer at a dilution of 1+2 from the supplied stock of primer. This step is important to prevent adaptor-dimer formation. The SR RT Primer hybridizes to the excess of the 3 ́ SR Adaptor (that remains free after the 3 ́ ligation reaction) and transforms the single stranded DNA adaptor into a double-stranded DNA molecule. dsDNAs are not substrates for ligation mediated by T4 RNA Ligase 1 and therefore do not ligate to the 5 ́ SR Adaptor in the subsequent ligation step. Following this 5”ligation for 1 hour at 25 degrees, a reverse transcription was carried out at 50 degrees for 1 hour using ProtoScript II Reverse Transcriptase. The samples were then amplified for 15 cycles and incorporating a barcode as part of the priming process. The samples were mixed into 3 pools,(mixing across groups) and size selected on a Sage Pippin cassette 2% DF marker E using a size range 120-300bp. The size selected material was purified with Ampure beads(1:1) (Agencourt). The pools were checked by Qubit analysis and Bioanalyser High Sensitivity chip.

text: Old

text: Mus musculus

text: Mus musculus

text: Serum

text: Mus musculus

characteristic: strain

text: Sample 23

characteristic: title


E-MTAB-4878:Sample 24

characteristic: ENA-CHECKLIST

characteristic: External Id

text: University of Liverpool

text: public

text: E-MTAB-4878:Sample 24

text: ArrayExpress

text: house mouse

text: Protocols: Skin removed from hind legs and stifle joints collected. Approximately 4mm each side of the joint was collected with no muscle included. destabilization of the medial meniscus (DMM) model of traumatic osteoarthritis 100ng of small RNA was added in a volume of 6ul together with 1ul of 3’SR adapter (diluted 1+2). This was heated at 70 degrees for 2minutes and cooled on ice. Ligation buffer and enzyme were added and the reaction incubated for 1 hour at 25 degrees. 2ul of Tobacco Acid Pyrophosphatase (TAP)(Epicentre) 5u/ul was added to each reaction and incubated at 37 degrees for 1 hour. Tobacco Acid Pyrophosphatase (TAP) hydrolyzes the phosphoric acid anhydride bonds in the triphosphate bridge of the cap structure found in most eukaryotic mRNA, releasing the cap nucleoside and generating a 5 ́-monophosphorylated terminus on the RNA molecule. This is inactivated in the subsequent step which heats the samples to 75 degrees for 5 minutes The samples were hybridized to the Reverse Transcription Primer at a dilution of 1+2 from the supplied stock of primer. This step is important to prevent adaptor-dimer formation. The SR RT Primer hybridizes to the excess of the 3 ́ SR Adaptor (that remains free after the 3 ́ ligation reaction) and transforms the single stranded DNA adaptor into a double-stranded DNA molecule. dsDNAs are not substrates for ligation mediated by T4 RNA Ligase 1 and therefore do not ligate to the 5 ́ SR Adaptor in the subsequent ligation step. Following this 5”ligation for 1 hour at 25 degrees, a reverse transcription was carried out at 50 degrees for 1 hour using ProtoScript II Reverse Transcriptase. The samples were then amplified for 15 cycles and incorporating a barcode as part of the priming process. The samples were mixed into 3 pools,(mixing across groups) and size selected on a Sage Pippin cassette 2% DF marker E using a size range 120-300bp. The size selected material was purified with Ampure beads(1:1) (Agencourt). The pools were checked by Qubit analysis and Bioanalyser High Sensitivity chip.

text: osteoarthritis

characteristic: disease

text: Mus musculus

text: Mus musculus

text: Joint

text: Mus musculus

text: Sample 24

text: destabilization of the medial meniscus

characteristic: treatment


E-MTAB-4878:Sample 25

text: University of Liverpool

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-4878:Sample 25

text: ArrayExpress

text: house mouse

text: osteoarthritis

text: Mus musculus

text: Mus musculus

text: Joint

text: Mus musculus

text: Sample 25

text: destabilization of the medial meniscus


E-MTAB-4878:Sample 26

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: University of Liverpool

text: public

characteristic: SRA accession

text: E-MTAB-4878:Sample 26

characteristic: Submitter Id

text: ArrayExpress

text: house mouse

text: osteoarthritis

text: Mus musculus

text: Mus musculus

text: Joint

text: Mus musculus

text: Sample 26

text: destabilization of the medial meniscus


E-MTAB-4878:Sample 27

text: University of Liverpool

text: public

text: E-MTAB-4878:Sample 27

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

characteristic: description

text: osteoarthritis

text: Mus musculus

text: Mus musculus

text: Joint

text: Mus musculus

text: Sample 27

text: destabilization of the medial meniscus


E-MTAB-4878:Sample 28

text: University of Liverpool

text: public

text: E-MTAB-4878:Sample 28

text: ArrayExpress

text: house mouse

text: osteoarthritis

text: Mus musculus

characteristic: organism

text: Mus musculus

text: Joint

characteristic: organism part

text: Mus musculus

characteristic: strain

text: Sample 28

text: destabilization of the medial meniscus


E-MTAB-4878:Sample 29

characteristic: ENA-CHECKLIST

characteristic: External Id

text: University of Liverpool

text: public

text: E-MTAB-4878:Sample 29

text: ArrayExpress

text: house mouse

text: osteoarthritis

text: Mus musculus

text: Mus musculus

text: Joint

text: Mus musculus

text: Sample 29

characteristic: title

text: destabilization of the medial meniscus


E-MTAB-4878:Sample 3

text: University of Liverpool

characteristic: INSDC center name

text: public

text: E-MTAB-4878:Sample 3

text: ArrayExpress

text: house mouse

text: Young

characteristic: developmental stage

text: Mus musculus

text: Mus musculus

text: Joint

text: Mus musculus

text: Sample 3


E-MTAB-4878:Sample 30

text: University of Liverpool

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-4878:Sample 30

text: ArrayExpress

text: house mouse

text: Protocols: Skin removed from hind legs and stifle joints collected. Approximately 4mm each side of the joint was collected with no muscle included. Sham DMM 100ng of small RNA was added in a volume of 6ul together with 1ul of 3’SR adapter (diluted 1+2). This was heated at 70 degrees for 2minutes and cooled on ice. Ligation buffer and enzyme were added and the reaction incubated for 1 hour at 25 degrees. 2ul of Tobacco Acid Pyrophosphatase (TAP)(Epicentre) 5u/ul was added to each reaction and incubated at 37 degrees for 1 hour. Tobacco Acid Pyrophosphatase (TAP) hydrolyzes the phosphoric acid anhydride bonds in the triphosphate bridge of the cap structure found in most eukaryotic mRNA, releasing the cap nucleoside and generating a 5 ́-monophosphorylated terminus on the RNA molecule. This is inactivated in the subsequent step which heats the samples to 75 degrees for 5 minutes The samples were hybridized to the Reverse Transcription Primer at a dilution of 1+2 from the supplied stock of primer. This step is important to prevent adaptor-dimer formation. The SR RT Primer hybridizes to the excess of the 3 ́ SR Adaptor (that remains free after the 3 ́ ligation reaction) and transforms the single stranded DNA adaptor into a double-stranded DNA molecule. dsDNAs are not substrates for ligation mediated by T4 RNA Ligase 1 and therefore do not ligate to the 5 ́ SR Adaptor in the subsequent ligation step. Following this 5”ligation for 1 hour at 25 degrees, a reverse transcription was carried out at 50 degrees for 1 hour using ProtoScript II Reverse Transcriptase. The samples were then amplified for 15 cycles and incorporating a barcode as part of the priming process. The samples were mixed into 3 pools,(mixing across groups) and size selected on a Sage Pippin cassette 2% DF marker E using a size range 120-300bp. The size selected material was purified with Ampure beads(1:1) (Agencourt). The pools were checked by Qubit analysis and Bioanalyser High Sensitivity chip.

text: osteoarthritis

characteristic: disease

text: Mus musculus

text: Mus musculus

text: Joint

text: Mus musculus

text: Sample 30

text: sham destabilization of the medial meniscus

characteristic: treatment


E-MTAB-4878:Sample 31

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: University of Liverpool

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-4878:Sample 31

text: ArrayExpress

text: house mouse

text: osteoarthritis

text: Mus musculus

text: Mus musculus

text: Joint

text: Mus musculus

text: Sample 31


E-MTAB-4878:Sample 32

text: University of Liverpool

text: public

text: E-MTAB-4878:Sample 32

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

characteristic: description

text: osteoarthritis

text: Mus musculus

text: Mus musculus

text: Joint

text: Mus musculus

text: Sample 32


E-MTAB-4878:Sample 33

characteristic: ENA-CHECKLIST

text: University of Liverpool

text: public

text: E-MTAB-4878:Sample 33

text: ArrayExpress

text: house mouse

text: Protocols: Serum collected from heart following sacrifice. destabilization of the medial meniscus (DMM) model of traumatic osteoarthritis 100ng of small RNA was added in a volume of 6ul together with 1ul of 3’SR adapter (diluted 1+2). This was heated at 70 degrees for 2minutes and cooled on ice. Ligation buffer and enzyme were added and the reaction incubated for 1 hour at 25 degrees. 2ul of Tobacco Acid Pyrophosphatase (TAP)(Epicentre) 5u/ul was added to each reaction and incubated at 37 degrees for 1 hour. Tobacco Acid Pyrophosphatase (TAP) hydrolyzes the phosphoric acid anhydride bonds in the triphosphate bridge of the cap structure found in most eukaryotic mRNA, releasing the cap nucleoside and generating a 5 ́-monophosphorylated terminus on the RNA molecule. This is inactivated in the subsequent step which heats the samples to 75 degrees for 5 minutes The samples were hybridized to the Reverse Transcription Primer at a dilution of 1+2 from the supplied stock of primer. This step is important to prevent adaptor-dimer formation. The SR RT Primer hybridizes to the excess of the 3 ́ SR Adaptor (that remains free after the 3 ́ ligation reaction) and transforms the single stranded DNA adaptor into a double-stranded DNA molecule. dsDNAs are not substrates for ligation mediated by T4 RNA Ligase 1 and therefore do not ligate to the 5 ́ SR Adaptor in the subsequent ligation step. Following this 5”ligation for 1 hour at 25 degrees, a reverse transcription was carried out at 50 degrees for 1 hour using ProtoScript II Reverse Transcriptase. The samples were then amplified for 15 cycles and incorporating a barcode as part of the priming process. The samples were mixed into 3 pools,(mixing across groups) and size selected on a Sage Pippin cassette 2% DF marker E using a size range 120-300bp. The size selected material was purified with Ampure beads(1:1) (Agencourt). The pools were checked by Qubit analysis and Bioanalyser High Sensitivity chip.

text: osteoarthritis

text: Mus musculus

characteristic: organism

text: Mus musculus

text: Serum

characteristic: organism part

text: Mus musculus

characteristic: strain

text: Sample 33

text: destabilization of the medial meniscus


E-MTAB-4878:Sample 34

characteristic: External Id

text: University of Liverpool

characteristic: INSDC center name

text: public

text: E-MTAB-4878:Sample 34

text: ArrayExpress

text: house mouse

text: osteoarthritis

text: Mus musculus

text: Mus musculus

text: Serum

text: Mus musculus

text: Sample 34

characteristic: title

text: destabilization of the medial meniscus


E-MTAB-4878:Sample 35

text: University of Liverpool

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-4878:Sample 35

text: ArrayExpress

text: house mouse

text: osteoarthritis

characteristic: disease

text: Mus musculus

text: Mus musculus

text: Serum

text: Mus musculus

text: Sample 35

text: destabilization of the medial meniscus


E-MTAB-4878:Sample 36

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: University of Liverpool

text: public

text: E-MTAB-4878:Sample 36

text: ArrayExpress

text: house mouse

text: osteoarthritis

text: Mus musculus

text: Mus musculus

text: Serum

text: Mus musculus

text: Sample 36

text: destabilization of the medial meniscus

characteristic: treatment


E-MTAB-4878:Sample 37

text: University of Liverpool

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-4878:Sample 37

characteristic: Submitter Id

text: ArrayExpress

text: house mouse

text: osteoarthritis

text: Mus musculus

text: Mus musculus

text: Serum

text: Mus musculus

text: Sample 37

text: destabilization of the medial meniscus


E-MTAB-4878:Sample 38

text: University of Liverpool

text: public

text: E-MTAB-4878:Sample 38

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

characteristic: description

text: osteoarthritis

text: Mus musculus

text: Mus musculus

text: Serum

text: Mus musculus

text: Sample 38

text: destabilization of the medial meniscus


E-MTAB-4878:Sample 39

characteristic: ENA-CHECKLIST

text: University of Liverpool

text: public

text: E-MTAB-4878:Sample 39

text: ArrayExpress

text: house mouse

text: Protocols: Serum collected from heart following sacrifice. destabilization of the medial meniscus (DMM) model of traumatic osteoarthritis Sham DMM 100ng of small RNA was added in a volume of 6ul together with 1ul of 3’SR adapter (diluted 1+2). This was heated at 70 degrees for 2minutes and cooled on ice. Ligation buffer and enzyme were added and the reaction incubated for 1 hour at 25 degrees. 2ul of Tobacco Acid Pyrophosphatase (TAP)(Epicentre) 5u/ul was added to each reaction and incubated at 37 degrees for 1 hour. Tobacco Acid Pyrophosphatase (TAP) hydrolyzes the phosphoric acid anhydride bonds in the triphosphate bridge of the cap structure found in most eukaryotic mRNA, releasing the cap nucleoside and generating a 5 ́-monophosphorylated terminus on the RNA molecule. This is inactivated in the subsequent step which heats the samples to 75 degrees for 5 minutes The samples were hybridized to the Reverse Transcription Primer at a dilution of 1+2 from the supplied stock of primer. This step is important to prevent adaptor-dimer formation. The SR RT Primer hybridizes to the excess of the 3 ́ SR Adaptor (that remains free after the 3 ́ ligation reaction) and transforms the single stranded DNA adaptor into a double-stranded DNA molecule. dsDNAs are not substrates for ligation mediated by T4 RNA Ligase 1 and therefore do not ligate to the 5 ́ SR Adaptor in the subsequent ligation step. Following this 5”ligation for 1 hour at 25 degrees, a reverse transcription was carried out at 50 degrees for 1 hour using ProtoScript II Reverse Transcriptase. The samples were then amplified for 15 cycles and incorporating a barcode as part of the priming process. The samples were mixed into 3 pools,(mixing across groups) and size selected on a Sage Pippin cassette 2% DF marker E using a size range 120-300bp. The size selected material was purified with Ampure beads(1:1) (Agencourt). The pools were checked by Qubit analysis and Bioanalyser High Sensitivity chip.

text: osteoarthritis

text: Mus musculus

characteristic: organism

text: Mus musculus

text: Serum

characteristic: organism part

text: Mus musculus

text: Sample 39


E-MTAB-4878:Sample 4

characteristic: External Id

text: University of Liverpool

characteristic: INSDC center name

text: public

text: E-MTAB-4878:Sample 4

text: ArrayExpress

text: house mouse

text: Young

characteristic: developmental stage

text: Mus musculus

text: Mus musculus

text: Joint

text: Mus musculus

characteristic: strain

text: Sample 4


E-MTAB-4878:Sample 40

text: University of Liverpool

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-4878:Sample 40

text: ArrayExpress

text: house mouse

text: osteoarthritis

characteristic: disease

text: Mus musculus

text: Mus musculus

text: Serum

text: Mus musculus

text: Sample 40

characteristic: title


E-MTAB-4878:Sample 41

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: University of Liverpool

text: public

characteristic: INSDC status

text: E-MTAB-4878:Sample 41

text: ArrayExpress

text: house mouse

text: osteoarthritis

text: Mus musculus

text: Mus musculus

text: Serum

text: Mus musculus

text: Sample 41

characteristic: treatment


E-MTAB-4878:Sample 5

text: University of Liverpool

text: public

characteristic: SRA accession

text: E-MTAB-4878:Sample 5

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

text: Protocols: Skin removed from hind legs and stifle joints collected. Approximately 4mm each side of the joint was collected with no muscle included. young normal donor 100ng of small RNA was added in a volume of 6ul together with 1ul of 3’SR adapter (diluted 1+2). This was heated at 70 degrees for 2minutes and cooled on ice. Ligation buffer and enzyme were added and the reaction incubated for 1 hour at 25 degrees. 2ul of Tobacco Acid Pyrophosphatase (TAP)(Epicentre) 5u/ul was added to each reaction and incubated at 37 degrees for 1 hour. Tobacco Acid Pyrophosphatase (TAP) hydrolyzes the phosphoric acid anhydride bonds in the triphosphate bridge of the cap structure found in most eukaryotic mRNA, releasing the cap nucleoside and generating a 5 ́-monophosphorylated terminus on the RNA molecule. This is inactivated in the subsequent step which heats the samples to 75 degrees for 5 minutes The samples were hybridized to the Reverse Transcription Primer at a dilution of 1+2 from the supplied stock of primer. This step is important to prevent adaptor-dimer formation. The SR RT Primer hybridizes to the excess of the 3 ́ SR Adaptor (that remains free after the 3 ́ ligation reaction) and transforms the single stranded DNA adaptor into a double-stranded DNA molecule. dsDNAs are not substrates for ligation mediated by T4 RNA Ligase 1 and therefore do not ligate to the 5 ́ SR Adaptor in the subsequent ligation step. Following this 5”ligation for 1 hour at 25 degrees, a reverse transcription was carried out at 50 degrees for 1 hour using ProtoScript II Reverse Transcriptase. The samples were then amplified for 15 cycles and incorporating a barcode as part of the priming process. The samples were mixed into 3 pools,(mixing across groups) and size selected on a Sage Pippin cassette 2% DF marker E using a size range 120-300bp. The size selected material was purified with Ampure beads(1:1) (Agencourt). The pools were checked by Qubit analysis and Bioanalyser High Sensitivity chip.

text: Young

text: Mus musculus

text: Mus musculus

text: Joint

text: Mus musculus

text: Sample 5


E-MTAB-4878:Sample 6

characteristic: ENA-CHECKLIST

text: University of Liverpool

text: public

text: E-MTAB-4878:Sample 6

text: ArrayExpress

text: house mouse

characteristic: description

text: Young

text: Mus musculus

characteristic: organism

text: Mus musculus

text: Joint

characteristic: organism part

text: Mus musculus

text: Sample 6


E-MTAB-4878:Sample 7

characteristic: External Id

text: University of Liverpool

characteristic: INSDC center name

text: public

text: E-MTAB-4878:Sample 7

text: ArrayExpress

text: house mouse

text: Old

characteristic: developmental stage

text: Mus musculus

text: Mus musculus

text: Joint

text: Mus musculus

characteristic: strain

text: Sample 7


E-MTAB-4878:Sample 8

text: University of Liverpool

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-4878:Sample 8

text: ArrayExpress

text: house mouse

text: Old

text: Mus musculus

text: Mus musculus

text: Joint

text: Mus musculus

text: Sample 8

characteristic: title


E-MTAB-4878:Sample 9

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: University of Liverpool

text: public

characteristic: INSDC status

text: E-MTAB-4878:Sample 9

text: ArrayExpress

text: house mouse

text: Old

text: Mus musculus

text: Mus musculus

text: Joint

text: Mus musculus

text: Sample 9


text: public

characteristic: SRA accession

tag: Namespace:Herlev and Gentofte Hospital, University of Copenhagen

characteristic: Submitter Id

characteristic: collection date

text: Denmark

characteristic: geographic location (country and/or sea)

text: diseased

characteristic: host health state

text: human

characteristic: host scientific name

characteristic: isolate

text: abdominal pus

characteristic: isolation source

text: Enterococcus faecalis

text: Enterococcus faecalis

text: Enterococcus faecalis in Copenhagen


characteristic: ENA-CHECKLIST

characteristic: External Id

text: Herlev and Gentofte Hospital, University of Copenhagen

characteristic: INSDC center name

text: public

text: Denmark

text: diseased

text: human

text: rectal swab

text: Enterococcus faecalis

characteristic: organism

text: Enterococcus faecalis

text: Enterococcus faecalis in Copenhagen


characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: Denmark

text: diseased

text: human

text: urine

text: Enterococcus faecalis

text: Enterococcus faecalis

text: Enterococcus faecalis in Copenhagen


characteristic: ENA-FIRST-PUBLIC

text: public

text: Denmark

text: diseased

text: human

text: blood

text: Enterococcus faecalis

text: Enterococcus faecalis

text: Enterococcus faecalis in Copenhagen

characteristic: title


characteristic: ENA-LAST-UPDATE

text: public

characteristic: INSDC status

text: Denmark

text: diseased

text: human

text: swab from catheter site

text: Enterococcus faecalis

text: Enterococcus faecalis

text: Enterococcus faecalis in Copenhagen


text: public

characteristic: SRA accession

characteristic: Submitter Id

text: Denmark

text: diseased

text: human

text: wound swab

text: Enterococcus faecalis

text: Enterococcus faecalis

text: Enterococcus faecalis in Copenhagen


text: public

characteristic: collection date

text: Denmark

characteristic: geographic location (country and/or sea)

text: diseased

text: human

text: bone tissue

text: Enterococcus faecalis

text: Enterococcus faecalis

text: Enterococcus faecalis in Copenhagen


text: UWASH

text: public

unit: m

characteristic: depth

text: T0 (DOY 180) Control nitrate addition cDNA

characteristic: description

text: Ice Sheet

characteristic: environment (biome)

text: Cryoconite hole

characteristic: environment (feature)

text: Cryoconite

characteristic: environment (material)

text: Greenland

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Southwest Greenland ice sheet

characteristic: geographic location (region and locality)

text: metagenome

characteristic: investigation type

text: unidentified prokaryotic organism

text: 341F, 806R

characteristic: pcr primers

text: Cryoconite nitrate fertilization

characteristic: project name

unit: g

characteristic: sample volume or weight for DNA extraction

text: unidentified prokaryotic organism

text: sediment

characteristic: sediment environmental package

text: MiSeq

characteristic: sequencing method


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UWASH

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: T0 (DOY 180) Low nitrate addition cDNA

text: Ice Sheet

text: Cryoconite hole

text: Cryoconite

text: Greenland

text: Southwest Greenland ice sheet

text: metagenome

text: unidentified prokaryotic organism

characteristic: organism

text: 341F, 806R

text: Cryoconite nitrate fertilization

text: unidentified prokaryotic organism

text: sediment

text: MiSeq

text: T0 (DOY 180) Low nitrate addition cDNA

characteristic: title


text: UWASH

text: public

characteristic: depth

text: T0 (DOY 180) High nitrate addition cDNA

characteristic: description

text: Ice Sheet

characteristic: environment (biome)

text: Cryoconite hole

characteristic: environment (feature)

text: Cryoconite

characteristic: environment (material)

text: Greenland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Southwest Greenland ice sheet

characteristic: geographic location (region and locality)

text: metagenome

characteristic: investigation type

text: unidentified prokaryotic organism

text: 341F, 806R

characteristic: pcr primers

text: Cryoconite nitrate fertilization

characteristic: project name

text: unidentified prokaryotic organism

text: sediment

text: MiSeq

text: T0 (DOY 180) High nitrate addition cDNA


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UWASH

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: T1 (DOY 187) Control nitrate addition cDNA

text: Ice Sheet

text: Cryoconite hole

text: Cryoconite

text: Greenland

text: Southwest Greenland ice sheet

text: metagenome

text: unidentified prokaryotic organism

text: 341F, 806R

text: Cryoconite nitrate fertilization

characteristic: sample volume or weight for DNA extraction

text: unidentified prokaryotic organism

text: sediment

characteristic: sediment environmental package

text: MiSeq

characteristic: sequencing method


text: UWASH

text: public

characteristic: collection date

characteristic: depth

text: T1 (DOY 187) Low nitrate addition cDNA

characteristic: description

text: Ice Sheet

characteristic: environment (biome)

text: Cryoconite hole

characteristic: environment (feature)

text: Cryoconite

characteristic: environment (material)

text: Greenland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Southwest Greenland ice sheet

characteristic: geographic location (region and locality)

text: metagenome

text: unidentified prokaryotic organism

characteristic: organism

text: 341F, 806R

text: Cryoconite nitrate fertilization

text: unidentified prokaryotic organism

text: sediment

text: MiSeq

text: T1 (DOY 187) Low nitrate addition cDNA

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UWASH

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: T1 (DOY 187) High nitrate addition cDNA

text: Ice Sheet

text: Cryoconite hole

text: Cryoconite

text: Greenland

text: Southwest Greenland ice sheet

text: metagenome

characteristic: investigation type

text: unidentified prokaryotic organism

text: 341F, 806R

characteristic: pcr primers

text: Cryoconite nitrate fertilization

characteristic: project name

text: unidentified prokaryotic organism

text: sediment

text: MiSeq

text: T1 (DOY 187) High nitrate addition cDNA


text: UWASH

text: public

characteristic: collection date

characteristic: depth

text: T2 (DOY 194) Control nitrate addition cDNA

characteristic: description

text: Ice Sheet

characteristic: environment (biome)

text: Cryoconite hole

characteristic: environment (feature)

text: Cryoconite

characteristic: environment (material)

text: Greenland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Southwest Greenland ice sheet

text: metagenome

text: unidentified prokaryotic organism

text: 341F, 806R

text: Cryoconite nitrate fertilization

characteristic: sample volume or weight for DNA extraction

text: unidentified prokaryotic organism

text: sediment

characteristic: sediment environmental package

text: MiSeq

characteristic: sequencing method


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UWASH

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: T2 (DOY 194) Low nitrate addition cDNA

text: Ice Sheet

text: Cryoconite hole

text: Cryoconite

text: Greenland

text: Southwest Greenland ice sheet

characteristic: geographic location (region and locality)

text: metagenome

text: unidentified prokaryotic organism

characteristic: organism

text: 341F, 806R

text: Cryoconite nitrate fertilization

text: unidentified prokaryotic organism

text: sediment

text: MiSeq

text: T2 (DOY 194) Low nitrate addition cDNA

characteristic: title


text: UWASH

text: public

characteristic: collection date

characteristic: depth

text: T2 (DOY 194) High nitrate addition cDNA

characteristic: description

text: Ice Sheet

characteristic: environment (biome)

text: Cryoconite hole

characteristic: environment (feature)

text: Cryoconite

characteristic: environment (material)

text: Greenland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Southwest Greenland ice sheet

text: metagenome

characteristic: investigation type

text: unidentified prokaryotic organism

text: 341F, 806R

characteristic: pcr primers

text: Cryoconite nitrate fertilization

characteristic: project name

text: unidentified prokaryotic organism

text: sediment

text: MiSeq

text: T2 (DOY 194) High nitrate addition cDNA


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UWASH

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: T3 (DOY 201) Control nitrate addition cDNA

text: Ice Sheet

text: Cryoconite hole

text: Cryoconite

text: Greenland

text: Southwest Greenland ice sheet

text: metagenome

text: unidentified prokaryotic organism

text: 341F, 806R

text: Cryoconite nitrate fertilization

characteristic: sample volume or weight for DNA extraction

text: unidentified prokaryotic organism

text: sediment

characteristic: sediment environmental package

text: MiSeq

characteristic: sequencing method


text: UWASH

text: public

characteristic: collection date

characteristic: depth

text: T3 (DOY 201) Low nitrate addition cDNA

characteristic: description

text: Ice Sheet

characteristic: environment (biome)

text: Cryoconite hole

characteristic: environment (feature)

text: Cryoconite

characteristic: environment (material)

text: Greenland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Southwest Greenland ice sheet

characteristic: geographic location (region and locality)

text: metagenome

text: unidentified prokaryotic organism

characteristic: organism

text: 341F, 806R

text: Cryoconite nitrate fertilization

text: unidentified prokaryotic organism

text: sediment

text: MiSeq

text: T3 (DOY 201) Low nitrate addition cDNA

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UWASH

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: T3 (DOY 201) High nitrate addition cDNA

text: Ice Sheet

text: Cryoconite hole

text: Cryoconite

text: Greenland

text: Southwest Greenland ice sheet

text: metagenome

characteristic: investigation type

text: unidentified prokaryotic organism

text: 341F, 806R

characteristic: pcr primers

text: Cryoconite nitrate fertilization

characteristic: project name

text: unidentified prokaryotic organism

text: sediment

text: MiSeq

text: T3 (DOY 201) High nitrate addition cDNA


text: UWASH

text: public

characteristic: collection date

characteristic: depth

text: T4 (DOY 208) Control nitrate addition cDNA

characteristic: description

text: Ice Sheet

characteristic: environment (biome)

text: Cryoconite hole

characteristic: environment (feature)

text: Cryoconite

characteristic: environment (material)

text: Greenland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Southwest Greenland ice sheet

text: metagenome

text: unidentified prokaryotic organism

text: 341F, 806R

text: Cryoconite nitrate fertilization

characteristic: sample volume or weight for DNA extraction

text: unidentified prokaryotic organism

text: sediment

characteristic: sediment environmental package

text: MiSeq

characteristic: sequencing method


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UWASH

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: T4 (DOY 208) Low nitrate addition cDNA

text: Ice Sheet

text: Cryoconite hole

text: Cryoconite

text: Greenland

text: Southwest Greenland ice sheet

characteristic: geographic location (region and locality)

text: metagenome

text: unidentified prokaryotic organism

characteristic: organism

text: 341F, 806R

text: Cryoconite nitrate fertilization

text: unidentified prokaryotic organism

text: sediment

text: MiSeq

text: T4 (DOY 208) Low nitrate addition cDNA

characteristic: title


text: UWASH

text: public

characteristic: collection date

characteristic: depth

text: T4 (DOY 208) High nitrate addition cDNA

characteristic: description

text: Ice Sheet

characteristic: environment (biome)

text: Cryoconite hole

characteristic: environment (feature)

text: Cryoconite

characteristic: environment (material)

text: Greenland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Southwest Greenland ice sheet

text: metagenome

characteristic: investigation type

text: unidentified prokaryotic organism

text: 341F, 806R

characteristic: pcr primers

text: Cryoconite nitrate fertilization

characteristic: project name

text: unidentified prokaryotic organism

text: sediment

text: MiSeq

text: T4 (DOY 208) High nitrate addition cDNA


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UWASH

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: T5 (DOY 214) Control nitrate addition cDNA

text: Ice Sheet

text: Cryoconite hole

text: Cryoconite

text: Greenland

text: Southwest Greenland ice sheet

text: metagenome

text: unidentified prokaryotic organism

text: 341F, 806R

text: Cryoconite nitrate fertilization

characteristic: sample volume or weight for DNA extraction

text: unidentified prokaryotic organism

text: sediment

characteristic: sediment environmental package

text: MiSeq

characteristic: sequencing method


text: UWASH

text: public

characteristic: collection date

characteristic: depth

text: T5 (DOY 214) Low nitrate addition cDNA

characteristic: description

text: Ice Sheet

characteristic: environment (biome)

text: Cryoconite hole

characteristic: environment (feature)

text: Cryoconite

characteristic: environment (material)

text: Greenland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Southwest Greenland ice sheet

characteristic: geographic location (region and locality)

text: metagenome

text: unidentified prokaryotic organism

characteristic: organism

text: 341F, 806R

text: Cryoconite nitrate fertilization

text: unidentified prokaryotic organism

text: sediment

text: MiSeq

text: T5 (DOY 214) Low nitrate addition cDNA

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UWASH

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: T5 (DOY 214) High nitrate addition cDNA

text: Ice Sheet

text: Cryoconite hole

text: Cryoconite

text: Greenland

text: Southwest Greenland ice sheet

text: metagenome

characteristic: investigation type

text: unidentified prokaryotic organism

text: 341F, 806R

characteristic: pcr primers

text: Cryoconite nitrate fertilization

characteristic: project name

text: unidentified prokaryotic organism

text: sediment

text: MiSeq

text: T5 (DOY 214) High nitrate addition cDNA


text: UWASH

text: public

characteristic: collection date

characteristic: depth

text: T6 (DOY 222) Control nitrate addition cDNA

characteristic: description

text: Ice Sheet

characteristic: environment (biome)

text: Cryoconite hole

characteristic: environment (feature)

text: Cryoconite

characteristic: environment (material)

text: Greenland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Southwest Greenland ice sheet

text: metagenome

text: unidentified prokaryotic organism

text: 341F, 806R

text: Cryoconite nitrate fertilization

characteristic: sample volume or weight for DNA extraction

text: unidentified prokaryotic organism

text: sediment

characteristic: sediment environmental package

text: MiSeq

characteristic: sequencing method


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UWASH

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: T6 (DOY 222) Low nitrate addition cDNA

text: Ice Sheet

text: Cryoconite hole

text: Cryoconite

text: Greenland

text: Southwest Greenland ice sheet

characteristic: geographic location (region and locality)

text: metagenome

text: unidentified prokaryotic organism

characteristic: organism

text: 341F, 806R

text: Cryoconite nitrate fertilization

text: unidentified prokaryotic organism

text: sediment

text: MiSeq

text: T6 (DOY 222) Low nitrate addition cDNA

characteristic: title


text: UWASH

text: public

characteristic: collection date

characteristic: depth

text: T6 (DOY 222) High nitrate addition cDNA

characteristic: description

text: Ice Sheet

characteristic: environment (biome)

text: Cryoconite hole

characteristic: environment (feature)

text: Cryoconite

characteristic: environment (material)

text: Greenland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Southwest Greenland ice sheet

text: metagenome

characteristic: investigation type

text: unidentified prokaryotic organism

text: 341F, 806R

characteristic: pcr primers

text: Cryoconite nitrate fertilization

characteristic: project name

text: unidentified prokaryotic organism

text: sediment

text: MiSeq

text: T6 (DOY 222) High nitrate addition cDNA