characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

characteristic: body site

characteristic: collection date

text: Pacific oyster

characteristic: common name

characteristic: description

characteristic: detailed conditions

text: diseased

characteristic: disease status

text: aquatic, marine

characteristic: environment (biome)

text: organic

characteristic: environment (feature)

text: organic material, bodily fluid

characteristic: environment (material)

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host body temperature

text: Pacific oyster

text: host-associated

text: mimarks-survey

characteristic: investigation type

text: adult

text: f: CGACGTGACT; r: ACGCTCGACA or f: CGACGTGACT; r: AGCACTGTAG

characteristic: multiplex identifiers

text: Crassostrea gigas

text: temperature acclimated

text: syringe

text: Crassostrea gigas

text: pyrosequencing

characteristic: sequencing method

text: 16S rRNA

text: C.gigas hemolymph microbiota WW_0_0

characteristic: title


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:ALFRED WEGENER INSTITUTE FOR POLAR AND MARINE RESE

characteristic: Submitter Id

text: f: adapter A + key (4bp): CGTATCGCCTCCCTCGCGCCATCAG; r: adapter B + key (4bp): CTATGCGCCTTGCCAGCCCGCTCAG

characteristic: adapters

unit: L

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

text: Pacific oyster

text: Experimental sample. Sample title code: [original temperature][experimental temperature]_[infection]_[time point]. For details see study description and 'perturbation' and 'detailed conditions' fields

text: 3 days after transfer from flowthrough to stationary seawater aquarium, temperature change from 8°C to 22°C and injection with soya-peptone medium

text: healthy

text: aquatic, marine

text: organic

text: organic material, bodily fluid

text: Germany

unit: DD

unit: ºC

text: Pacific oyster

characteristic: host common name

characteristic: host subject id

characteristic: host taxid

text: host-associated

characteristic: host-associated environmental package

text: mimarks-survey

text: adult

characteristic: life stage

text: Schleswig-Holstein, Wadden Sea (North Sea), island of Sylt, Kongishafen

characteristic: location details

text: f: ACAGTATATA; r: AGACGCACTC or f: ACAGTATATA; r: ATATCGCGAG

text: Crassostrea gigas

characteristic: organism

text: initial denaturation:94degC_1min; denaturation:94degC_40s;annealing:55degC_30s; elongation:72degC_30s; final elongation:72degC_2min; 35 cycles

characteristic: pcr conditions

text: f: linker (3bp) + 16S_338r: CATGCTGCCTCCCGTAGGAGT; r: linker (2bp) + 16S_27F: TCAGAGTTTGATCMTGGCTCAG

characteristic: pcr primers

text: temperature stressed, mock-infected

characteristic: perturbation

characteristic: project name

text: syringe

characteristic: sample collection device or method

text: Crassostrea gigas

text: pyrosequencing

text: seawater (local, supplied through cca 200 m long pipe, 30 um filtered and protein skimmed)

characteristic: substrate

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: temperature

text: C.gigas hemolymph microbiota CW_0_3


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ALFRED WEGENER INSTITUTE FOR POLAR AND MARINE RESE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

characteristic: body site

characteristic: collection date

text: Pacific oyster

characteristic: common name

characteristic: description

text: held in flowthrough aquarium, 8°C, well acclimated

characteristic: detailed conditions

text: healthy

characteristic: disease status

text: aquatic, marine

characteristic: environment (biome)

text: organic

characteristic: environment (feature)

text: organic material, bodily fluid

characteristic: environment (material)

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host body temperature

text: Pacific oyster

text: host-associated

text: mimarks-survey

characteristic: investigation type

text: adult

text: f: AGACTATACT; r: ACGCTCGACA or f: AGACTATACT; r: AGCACTGTAG

characteristic: multiplex identifiers

text: Crassostrea gigas

text: temperature acclimated

text: syringe

text: Crassostrea gigas

text: pyrosequencing

characteristic: sequencing method

text: 16S rRNA

text: C.gigas hemolymph microbiota CW_0_0

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

text: Pacific oyster

text: 7 days after transfer from flowthrough to stationary seawater aquarium (both 22°C) and injection with Vibrio sp. D29w strain

text: healthy

text: aquatic, marine

text: organic

text: organic material, bodily fluid

text: Germany

text: Pacific oyster

characteristic: host common name

characteristic: host subject id

characteristic: host taxid

text: host-associated

characteristic: host-associated environmental package

text: mimarks-survey

text: adult

characteristic: life stage

characteristic: location details

text: f: CGTATGCGAC; r: AGACGCACTC or f: CGTATGCGAC; r: ATATCGCGAG

text: Crassostrea gigas

characteristic: organism

characteristic: pcr conditions

characteristic: pcr primers

text: temperature acclimated, infected

characteristic: perturbation

characteristic: project name

text: syringe

characteristic: sample collection device or method

text: Crassostrea gigas

text: pyrosequencing

characteristic: substrate

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: temperature

text: C.gigas hemolymph microbiota WW_1_7


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

characteristic: body site

characteristic: collection date

text: Pacific oyster

characteristic: common name

characteristic: description

text: 7 days after transfer from flowthrough to stationary seawater aquarium (both 8°C) and injection with soya-peptone medium

characteristic: detailed conditions

text: healthy

characteristic: disease status

text: aquatic, marine

characteristic: environment (biome)

text: organic

characteristic: environment (feature)

text: organic material, bodily fluid

characteristic: environment (material)

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host body temperature

text: Pacific oyster

text: host-associated

text: mimarks-survey

characteristic: investigation type

text: adult

text: f: TGAGTCAGTA; r: AGACGCACTC or f: TGAGTCAGTA; r: ATATCGCGAG

characteristic: multiplex identifiers

text: Crassostrea gigas

text: temperature acclimated, mock-infected

text: syringe

text: Crassostrea gigas

text: pyrosequencing

characteristic: sequencing method

text: 16S rRNA

text: C.gigas hemolymph microbiota CC_0_7

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

text: Pacific oyster

text: 5 days after transfer from flowthrough to stationary seawater aquarium, temperature change from 8°C to 22°C and injection with soya-peptone medium

text: healthy

text: aquatic, marine

text: organic

text: organic material, bodily fluid

text: Germany

text: Pacific oyster

characteristic: host common name

characteristic: host subject id

characteristic: host taxid

text: host-associated

characteristic: host-associated environmental package

text: mimarks-survey

text: adult

characteristic: life stage

characteristic: location details

text: f: CTACGACTGC; r: ACGCTCGACA or f: CTACGACTGC; r: AGCACTGTAG

text: Crassostrea gigas

characteristic: organism

characteristic: pcr conditions

characteristic: pcr primers

text: temperature stressed, mock-infected

characteristic: perturbation

characteristic: project name

text: syringe

characteristic: sample collection device or method

text: Crassostrea gigas

text: pyrosequencing

characteristic: substrate

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: temperature

text: C.gigas hemolymph microbiota CW_0_5


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: f: adapter A + key (4bp): CGTATCGCCTCCCTCGCGCCATCAG; r: adapter B + key (4bp): CTATGCGCCTTGCCAGCCCGCTCAG

characteristic: adapters

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

characteristic: body site

characteristic: collection date

text: Pacific oyster

characteristic: common name

characteristic: description

text: 5 days after transfer from flowthrough to stationary seawater aquarium, temperature change from 22°C to 8°C and injection with Vibrio sp. D29w strain

characteristic: detailed conditions

text: diseased

characteristic: disease status

text: aquatic, marine

characteristic: environment (biome)

text: organic

characteristic: environment (feature)

text: organic material, bodily fluid

characteristic: environment (material)

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host body temperature

text: Pacific oyster

text: host-associated

text: mimarks-survey

characteristic: investigation type

text: adult

text: f: TCACGCGAGA; r: ACGCTCGACA or f: TCACGCGAGA; r: AGCACTGTAG

characteristic: multiplex identifiers

text: Crassostrea gigas

text: temperature stressed, infected

text: syringe

text: Crassostrea gigas

text: pyrosequencing

characteristic: sequencing method

text: 16S rRNA

text: C.gigas hemolymph microbiota WC_1_5

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

text: Pacific oyster

text: Experimental sample. Sample title code: [original temperature][experimental temperature]_[infection]_[time point]. For details see study description and 'perturbation' and 'detailed conditions' fields

text: 5 days after transfer from flowthrough to stationary seawater aquarium (both 8°C) and injection with Vibrio sp. D29w strain

text: healthy

text: aquatic, marine

text: organic

text: organic material, bodily fluid

text: Germany

text: Pacific oyster

characteristic: host common name

characteristic: host subject id

characteristic: host taxid

text: host-associated

characteristic: host-associated environmental package

text: mimarks-survey

text: adult

characteristic: life stage

text: Schleswig-Holstein, Wadden Sea (North Sea), island of Sylt, Kongishafen

characteristic: location details

text: f: AGTGCTACGA; r: ACGCTCGACA or f: AGTGCTACGA; r: AGCACTGTAG

text: Crassostrea gigas

characteristic: organism

characteristic: pcr conditions

characteristic: pcr primers

text: temperature acclimated, infected

characteristic: perturbation

characteristic: project name

text: syringe

characteristic: sample collection device or method

text: Crassostrea gigas

text: pyrosequencing

characteristic: substrate

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: temperature

text: C.gigas hemolymph microbiota CC_1_5


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

characteristic: body site

characteristic: collection date

text: Pacific oyster

characteristic: common name

characteristic: description

text: 3 days after transfer from flowthrough to stationary seawater aquarium (both 22°C) and injection with soya-peptone medium

characteristic: detailed conditions

text: dead

characteristic: disease status

text: aquatic, marine

characteristic: environment (biome)

text: organic

characteristic: environment (feature)

text: organic material, bodily fluid

characteristic: environment (material)

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host body temperature

text: Pacific oyster

text: host-associated

text: mimarks-survey

characteristic: investigation type

text: adult

text: f: CGACGTGACT; r: AGACGCACTC or f: CGACGTGACT; r: ATATCGCGAG

characteristic: multiplex identifiers

text: Crassostrea gigas

text: initial denaturation:94degC_1min; denaturation:94degC_40s;annealing:55degC_30s; elongation:72degC_30s; final elongation:72degC_2min; 35 cycles

text: f: linker (3bp) + 16S_338r: CATGCTGCCTCCCGTAGGAGT; r: linker (2bp) + 16S_27F: TCAGAGTTTGATCMTGGCTCAG

text: temperature acclimated, mock-infected

text: syringe

text: Crassostrea gigas

text: pyrosequencing

characteristic: sequencing method

text: seawater (local, supplied through cca 200 m long pipe, 30 um filtered and protein skimmed)

text: 16S rRNA

text: C.gigas hemolymph microbiota WW_0_3

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ALFRED WEGENER INSTITUTE FOR POLAR AND MARINE RESE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

text: Pacific oyster

text: 3 days after transfer from flowthrough to stationary seawater aquarium (both 8°C) and injection with Vibrio sp. D29w strain

text: healthy

text: aquatic, marine

text: organic

text: organic material, bodily fluid

text: Germany

text: Pacific oyster

characteristic: host common name

characteristic: host subject id

characteristic: host taxid

text: host-associated

characteristic: host-associated environmental package

text: mimarks-survey

text: adult

characteristic: life stage

characteristic: location details

text: f: TAGAGACGAG; r: AGACGCACTC or f: TAGAGACGAG; r: ATATCGCGAG

text: Crassostrea gigas

characteristic: organism

characteristic: pcr conditions

characteristic: pcr primers

text: temperature acclimated, infected

characteristic: perturbation

characteristic: project name

text: syringe

characteristic: sample collection device or method

text: Crassostrea gigas

text: pyrosequencing

characteristic: substrate

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: temperature

text: C.gigas hemolymph microbiota CC_1_3


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

characteristic: body site

characteristic: collection date

text: Pacific oyster

characteristic: common name

characteristic: description

text: 7 days after transfer from flowthrough to stationary seawater aquarium (both 8°C) and injection with soya-peptone medium

characteristic: detailed conditions

text: healthy

characteristic: disease status

text: aquatic, marine

characteristic: environment (biome)

text: organic

characteristic: environment (feature)

text: organic material, bodily fluid

characteristic: environment (material)

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host body temperature

text: Pacific oyster

text: host-associated

text: mimarks-survey

characteristic: investigation type

text: adult

text: f: TATGCTAGTA; r: AGACGCACTC or f: TATGCTAGTA; r: ATATCGCGAG

characteristic: multiplex identifiers

text: Crassostrea gigas

text: temperature acclimated, mock-infected

text: syringe

text: Crassostrea gigas

text: pyrosequencing

characteristic: sequencing method

text: 16S rRNA

text: C.gigas hemolymph microbiota CC_0_7

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: f: adapter A + key (4bp): CGTATCGCCTCCCTCGCGCCATCAG; r: adapter B + key (4bp): CTATGCGCCTTGCCAGCCCGCTCAG

characteristic: adapters

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

text: Pacific oyster

text: 5 days after transfer from flowthrough to stationary seawater aquarium, temperature change from 8°C to 22°C and injection with soya-peptone medium

text: healthy

text: aquatic, marine

text: organic

text: organic material, bodily fluid

text: Germany

text: Pacific oyster

characteristic: host common name

characteristic: host subject id

characteristic: host taxid

text: host-associated

characteristic: host-associated environmental package

text: mimarks-survey

text: adult

characteristic: life stage

characteristic: location details

text: f: ATCTACTGAC; r: ACGCTCGACA or f: ATCTACTGAC; r: AGCACTGTAG

text: Crassostrea gigas

characteristic: organism

characteristic: pcr conditions

characteristic: pcr primers

text: temperature stressed, mock-infected

characteristic: perturbation

characteristic: project name

text: syringe

characteristic: sample collection device or method

text: Crassostrea gigas

text: pyrosequencing

characteristic: substrate

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: temperature

text: C.gigas hemolymph microbiota CW_0_5


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

characteristic: body site

characteristic: collection date

text: Pacific oyster

characteristic: common name

text: Experimental sample. Sample title code: [original temperature][experimental temperature]_[infection]_[time point]. For details see study description and 'perturbation' and 'detailed conditions' fields

characteristic: description

text: held in flowthrough aquarium, 8°C, well acclimated

characteristic: detailed conditions

text: healthy

characteristic: disease status

text: aquatic, marine

characteristic: environment (biome)

text: organic

characteristic: environment (feature)

text: organic material, bodily fluid

characteristic: environment (material)

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host body temperature

text: Pacific oyster

text: host-associated

text: mimarks-survey

characteristic: investigation type

text: adult

text: f: ACGCGAGTAT; r: ACGCTCGACA or f: ACGCGAGTAT; r: AGCACTGTAG

characteristic: multiplex identifiers

text: Crassostrea gigas

text: temperature acclimated

text: syringe

text: Crassostrea gigas

text: pyrosequencing

characteristic: sequencing method

text: 16S rRNA

text: C.gigas hemolymph microbiota CW_0_0

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

text: Pacific oyster

text: 7 days after transfer from flowthrough to stationary seawater aquarium (both 22°C) and injection with soya-peptone medium

text: healthy

text: aquatic, marine

text: organic

text: organic material, bodily fluid

text: Germany

text: Pacific oyster

characteristic: host common name

characteristic: host subject id

characteristic: host taxid

text: host-associated

characteristic: host-associated environmental package

text: mimarks-survey

text: adult

characteristic: life stage

text: Schleswig-Holstein, Wadden Sea (North Sea), island of Sylt, Kongishafen

characteristic: location details

text: f: CGACACTATC; r: AGACGCACTC or f: CGACACTATC; r: ATATCGCGAG

text: Crassostrea gigas

characteristic: organism

characteristic: pcr conditions

characteristic: pcr primers

text: temperature acclimated, mock-infected

characteristic: perturbation

characteristic: project name

text: syringe

characteristic: sample collection device or method

text: Crassostrea gigas

text: pyrosequencing

characteristic: substrate

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: temperature

text: C.gigas hemolymph microbiota WW_0_7


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

characteristic: body site

characteristic: collection date

text: Pacific oyster

characteristic: common name

characteristic: description

characteristic: detailed conditions

text: healthy

characteristic: disease status

text: aquatic, marine

characteristic: environment (biome)

text: organic

characteristic: environment (feature)

text: organic material, bodily fluid

characteristic: environment (material)

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host body temperature

text: Pacific oyster

text: host-associated

text: mimarks-survey

characteristic: investigation type

text: adult

text: f: CGTCTAGTAC; r: ACGCTCGACA or f: CGTCTAGTAC; r: AGCACTGTAG

characteristic: multiplex identifiers

text: Crassostrea gigas

text: initial denaturation:94degC_1min; denaturation:94degC_40s;annealing:55degC_30s; elongation:72degC_30s; final elongation:72degC_2min; 35 cycles

text: temperature acclimated

text: syringe

text: Crassostrea gigas

text: pyrosequencing

characteristic: sequencing method

text: 16S rRNA

text: C.gigas hemolymph microbiota CC_0_0

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

text: Pacific oyster

text: 3 days after transfer from flowthrough to stationary seawater aquarium, temperature change from 8°C to 22°C and injection with Vibrio sp. D29w strain

text: dead

text: aquatic, marine

text: organic

text: organic material, bodily fluid

text: Germany

text: Pacific oyster

characteristic: host common name

characteristic: host subject id

characteristic: host taxid

text: host-associated

characteristic: host-associated environmental package

text: mimarks-survey

text: adult

characteristic: life stage

characteristic: location details

text: f: TCGATCACGT; r: AGACGCACTC or f: TCGATCACGT; r: ATATCGCGAG

text: Crassostrea gigas

characteristic: organism

characteristic: pcr conditions

text: f: linker (3bp) + 16S_338r: CATGCTGCCTCCCGTAGGAGT; r: linker (2bp) + 16S_27F: TCAGAGTTTGATCMTGGCTCAG

characteristic: pcr primers

text: temperature stressed, infected

characteristic: perturbation

characteristic: project name

text: syringe

characteristic: sample collection device or method

text: Crassostrea gigas

text: pyrosequencing

text: seawater (local, supplied through cca 200 m long pipe, 30 um filtered and protein skimmed)

characteristic: substrate

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: temperature

text: C.gigas hemolymph microbiota CW_1_3


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ALFRED WEGENER INSTITUTE FOR POLAR AND MARINE RESE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

characteristic: body site

characteristic: collection date

text: Pacific oyster

characteristic: common name

characteristic: description

text: held in flowthrough aquarium, 22°C, well acclimated

characteristic: detailed conditions

text: healthy

characteristic: disease status

text: aquatic, marine

characteristic: environment (biome)

text: organic

characteristic: environment (feature)

text: organic material, bodily fluid

characteristic: environment (material)

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host body temperature

text: Pacific oyster

text: host-associated

text: mimarks-survey

characteristic: investigation type

text: adult

text: f: ACTGTACAGT; r: ACGCTCGACA or f: ACTGTACAGT; r: AGCACTGTAG

characteristic: multiplex identifiers

text: Crassostrea gigas

text: temperature acclimated

text: syringe

text: Crassostrea gigas

text: pyrosequencing

characteristic: sequencing method

text: 16S rRNA

text: C.gigas hemolymph microbiota WW_0_0

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: f: adapter A + key (4bp): CGTATCGCCTCCCTCGCGCCATCAG; r: adapter B + key (4bp): CTATGCGCCTTGCCAGCCCGCTCAG

characteristic: adapters

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

text: Pacific oyster

text: healthy

text: aquatic, marine

text: organic

text: organic material, bodily fluid

text: Germany

text: Pacific oyster

characteristic: host common name

characteristic: host subject id

characteristic: host taxid

text: host-associated

characteristic: host-associated environmental package

text: mimarks-survey

text: adult

characteristic: life stage

characteristic: location details

text: f: ACTAGCAGTA; r: ACGCTCGACA or f: ACTAGCAGTA; r: AGCACTGTAG

text: Crassostrea gigas

characteristic: organism

characteristic: pcr conditions

characteristic: pcr primers

text: temperature acclimated

characteristic: perturbation

characteristic: project name

text: syringe

characteristic: sample collection device or method

text: Crassostrea gigas

text: pyrosequencing

characteristic: substrate

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: temperature

text: C.gigas hemolymph microbiota CW_1_0


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

characteristic: body site

characteristic: collection date

text: Pacific oyster

characteristic: common name

text: Experimental sample. Sample title code: [original temperature][experimental temperature]_[infection]_[time point]. For details see study description and 'perturbation' and 'detailed conditions' fields

characteristic: description

characteristic: detailed conditions

text: diseased

characteristic: disease status

text: aquatic, marine

characteristic: environment (biome)

text: organic

characteristic: environment (feature)

text: organic material, bodily fluid

characteristic: environment (material)

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host body temperature

text: Pacific oyster

text: host-associated

text: mimarks-survey

characteristic: investigation type

text: adult

text: f: ACGCGATCGA; r: ACGCTCGACA or f: ACGCGATCGA; r: AGCACTGTAG

characteristic: multiplex identifiers

text: Crassostrea gigas

text: temperature acclimated

text: syringe

text: Crassostrea gigas

text: pyrosequencing

characteristic: sequencing method

text: 16S rRNA

text: C.gigas hemolymph microbiota WW_1_0

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

text: Pacific oyster

text: 7 days after transfer from flowthrough to stationary seawater aquarium, temperature change from 22°C to 8°C and injection with Vibrio sp. D29w strain

text: dead

text: aquatic, marine

text: organic

text: organic material, bodily fluid

text: Germany

text: Pacific oyster

characteristic: host common name

characteristic: host subject id

characteristic: host taxid

text: host-associated

characteristic: host-associated environmental package

text: mimarks-survey

text: adult

characteristic: life stage

characteristic: location details

text: f: TCGCTGCGTA; r: AGACGCACTC or f: TCGCTGCGTA; r: ATATCGCGAG

text: Crassostrea gigas

characteristic: organism

characteristic: pcr conditions

characteristic: pcr primers

text: temperature stressed, infected

characteristic: perturbation

characteristic: project name

text: syringe

characteristic: sample collection device or method

text: Crassostrea gigas

text: pyrosequencing

characteristic: substrate

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: temperature

text: C.gigas hemolymph microbiota WC_1_7


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

characteristic: body site

characteristic: collection date

text: Pacific oyster

characteristic: common name

characteristic: description

text: 5 days after transfer from flowthrough to stationary seawater aquarium (both 22°C) and injection with Vibrio sp. D29w strain

characteristic: detailed conditions

text: diseased

characteristic: disease status

text: aquatic, marine

characteristic: environment (biome)

text: organic

characteristic: environment (feature)

text: organic material, bodily fluid

characteristic: environment (material)

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host body temperature

text: Pacific oyster

text: host-associated

text: mimarks-survey

characteristic: investigation type

text: adult

text: Schleswig-Holstein, Wadden Sea (North Sea), island of Sylt, Kongishafen

text: f: ACATGACGAC; r: ACGCTCGACA or f: ACATGACGAC; r: AGCACTGTAG

characteristic: multiplex identifiers

text: Crassostrea gigas

text: temperature acclimated, infected

text: syringe

text: Crassostrea gigas

text: pyrosequencing

characteristic: sequencing method

text: 16S rRNA

text: C.gigas hemolymph microbiota WW_1_5

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

text: Pacific oyster

text: 3 days after transfer from flowthrough to stationary seawater aquarium (both 8°C) and injection with Vibrio sp. D29w strain

text: diseased

text: aquatic, marine

text: organic

text: organic material, bodily fluid

text: Germany

text: Pacific oyster

characteristic: host common name

characteristic: host subject id

characteristic: host taxid

text: host-associated

characteristic: host-associated environmental package

text: mimarks-survey

text: adult

characteristic: life stage

characteristic: location details

text: f: CGAGAGATAC; r: AGACGCACTC or f: CGAGAGATAC; r: ATATCGCGAG

text: Crassostrea gigas

characteristic: organism

text: initial denaturation:94degC_1min; denaturation:94degC_40s;annealing:55degC_30s; elongation:72degC_30s; final elongation:72degC_2min; 35 cycles

characteristic: pcr conditions

characteristic: pcr primers

text: temperature acclimated, infected

characteristic: perturbation

characteristic: project name

text: syringe

characteristic: sample collection device or method

text: Crassostrea gigas

text: pyrosequencing

characteristic: substrate

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: temperature

text: C.gigas hemolymph microbiota CC_1_3


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

characteristic: body site

characteristic: collection date

text: Pacific oyster

characteristic: common name

characteristic: description

characteristic: detailed conditions

text: healthy

characteristic: disease status

text: aquatic, marine

characteristic: environment (biome)

text: organic

characteristic: environment (feature)

text: organic material, bodily fluid

characteristic: environment (material)

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host body temperature

text: Pacific oyster

text: host-associated

text: mimarks-survey

characteristic: investigation type

text: adult

text: f: CGTAGACTAG; r: ACGCTCGACA or f: CGTAGACTAG; r: AGCACTGTAG

characteristic: multiplex identifiers

text: Crassostrea gigas

text: f: linker (3bp) + 16S_338r: CATGCTGCCTCCCGTAGGAGT; r: linker (2bp) + 16S_27F: TCAGAGTTTGATCMTGGCTCAG

text: temperature acclimated

text: syringe

text: Crassostrea gigas

text: pyrosequencing

characteristic: sequencing method

text: 16S rRNA

text: C.gigas hemolymph microbiota WC_0_0

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ALFRED WEGENER INSTITUTE FOR POLAR AND MARINE RESE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

text: Pacific oyster

text: 3 days after transfer from flowthrough to stationary seawater aquarium, temperature change from 22°C to 8°C and injection with Vibrio sp. D29w strain

text: healthy

text: aquatic, marine

text: organic

text: organic material, bodily fluid

text: Germany

text: Pacific oyster

characteristic: host common name

characteristic: host subject id

characteristic: host taxid

text: host-associated

characteristic: host-associated environmental package

text: mimarks-survey

text: adult

characteristic: life stage

characteristic: location details

text: f: ACGACTACAG; r: AGACGCACTC or f: ACGACTACAG; r: ATATCGCGAG

text: Crassostrea gigas

characteristic: organism

characteristic: pcr conditions

characteristic: pcr primers

text: temperature stressed, infected

characteristic: perturbation

characteristic: project name

text: syringe

characteristic: sample collection device or method

text: Crassostrea gigas

text: pyrosequencing

text: seawater (local, supplied through cca 200 m long pipe, 30 um filtered and protein skimmed)

characteristic: substrate

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: temperature

text: C.gigas hemolymph microbiota WC_1_3


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: f: adapter A + key (4bp): CGTATCGCCTCCCTCGCGCCATCAG; r: adapter B + key (4bp): CTATGCGCCTTGCCAGCCCGCTCAG

characteristic: adapters

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

characteristic: body site

characteristic: collection date

text: Pacific oyster

characteristic: common name

characteristic: description

text: held in flowthrough aquarium, 22°C, well acclimated

characteristic: detailed conditions

text: healthy

characteristic: disease status

text: aquatic, marine

characteristic: environment (biome)

text: organic

characteristic: environment (feature)

text: organic material, bodily fluid

characteristic: environment (material)

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host body temperature

text: Pacific oyster

text: host-associated

text: mimarks-survey

characteristic: investigation type

text: adult

text: f: TGATACGTCT; r: ACGCTCGACA or f: TGATACGTCT; r: AGCACTGTAG

characteristic: multiplex identifiers

text: Crassostrea gigas

text: temperature acclimated

text: syringe

text: Crassostrea gigas

text: pyrosequencing

characteristic: sequencing method

text: 16S rRNA

text: C.gigas hemolymph microbiota WC_0_0

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

text: Pacific oyster

text: Experimental sample. Sample title code: [original temperature][experimental temperature]_[infection]_[time point]. For details see study description and 'perturbation' and 'detailed conditions' fields

text: 5 days after transfer from flowthrough to stationary seawater aquarium, temperature change from 22°C to 8°C and injection with Vibrio sp. D29w strain

text: healthy

text: aquatic, marine

text: organic

text: organic material, bodily fluid

text: Germany

text: Pacific oyster

characteristic: host common name

characteristic: host subject id

characteristic: host taxid

text: host-associated

characteristic: host-associated environmental package

text: mimarks-survey

text: adult

characteristic: life stage

characteristic: location details

text: f: TGTAGTGTGA; r: ACGCTCGACA or f: TGTAGTGTGA; r: AGCACTGTAG

text: Crassostrea gigas

characteristic: organism

characteristic: pcr conditions

characteristic: pcr primers

text: temperature stressed, infected

characteristic: perturbation

characteristic: project name

text: syringe

characteristic: sample collection device or method

text: Crassostrea gigas

text: pyrosequencing

characteristic: substrate

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: temperature

text: C.gigas hemolymph microbiota WC_1_5


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

characteristic: body site

characteristic: collection date

text: Pacific oyster

characteristic: common name

characteristic: description

text: 3 days after transfer from flowthrough to stationary seawater aquarium (both 8°C) and injection with soya-peptone medium

characteristic: detailed conditions

text: healthy

characteristic: disease status

text: aquatic, marine

characteristic: environment (biome)

text: organic

characteristic: environment (feature)

text: organic material, bodily fluid

characteristic: environment (material)

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host body temperature

text: Pacific oyster

text: host-associated

text: mimarks-survey

characteristic: investigation type

text: adult

text: Schleswig-Holstein, Wadden Sea (North Sea), island of Sylt, Kongishafen

text: f: CATAGTAGTG; r: AGACGCACTC or f: CATAGTAGTG; r: ATATCGCGAG

characteristic: multiplex identifiers

text: Crassostrea gigas

text: temperature acclimated, mock-infected

text: syringe

text: Crassostrea gigas

text: pyrosequencing

characteristic: sequencing method

text: 16S rRNA

text: C.gigas hemolymph microbiota CC_0_3

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

text: Pacific oyster

text: 5 days after transfer from flowthrough to stationary seawater aquarium (both 8°C) and injection with Vibrio sp. D29w strain

text: diseased

text: aquatic, marine

text: organic

text: organic material, bodily fluid

text: Germany

text: Pacific oyster

characteristic: host common name

characteristic: host subject id

characteristic: host taxid

text: host-associated

characteristic: host-associated environmental package

text: mimarks-survey

text: adult

characteristic: life stage

characteristic: location details

text: f: TGTCACACGA; r: ACGCTCGACA or f: TGTCACACGA; r: AGCACTGTAG

text: Crassostrea gigas

characteristic: organism

characteristic: pcr conditions

characteristic: pcr primers

text: temperature acclimated, infected

characteristic: perturbation

characteristic: project name

text: syringe

characteristic: sample collection device or method

text: Crassostrea gigas

text: pyrosequencing

characteristic: substrate

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: temperature

text: C.gigas hemolymph microbiota CC_1_5


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

characteristic: body site

characteristic: collection date

text: Pacific oyster

characteristic: common name

characteristic: description

text: 3 days after transfer from flowthrough to stationary seawater aquarium, temperature change from 22°C to 8°C and injection with soya-peptone medium

characteristic: detailed conditions

text: healthy

characteristic: disease status

text: aquatic, marine

characteristic: environment (biome)

text: organic

characteristic: environment (feature)

text: organic material, bodily fluid

characteristic: environment (material)

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host body temperature

text: Pacific oyster

text: host-associated

text: mimarks-survey

characteristic: investigation type

text: adult

text: f: CGTAGACTAG; r: AGACGCACTC or f: CGTAGACTAG; r: ATATCGCGAG

characteristic: multiplex identifiers

text: Crassostrea gigas

text: initial denaturation:94degC_1min; denaturation:94degC_40s;annealing:55degC_30s; elongation:72degC_30s; final elongation:72degC_2min; 35 cycles

text: temperature stressed, mock-infected

text: syringe

text: Crassostrea gigas

text: pyrosequencing

characteristic: sequencing method

text: 16S rRNA

text: C.gigas hemolymph microbiota WC_0_3

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ALFRED WEGENER INSTITUTE FOR POLAR AND MARINE RESE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

text: Pacific oyster

text: 5 days after transfer from flowthrough to stationary seawater aquarium, temperature change from 8°C to 22°C and injection with soya-peptone medium

text: healthy

text: aquatic, marine

text: organic

text: organic material, bodily fluid

text: Germany

text: Pacific oyster

characteristic: host common name

characteristic: host subject id

characteristic: host taxid

text: host-associated

characteristic: host-associated environmental package

text: mimarks-survey

text: adult

characteristic: life stage

characteristic: location details

text: f: ACGTCTCATC; r: ACGCTCGACA or f: ACGTCTCATC; r: AGCACTGTAG

text: Crassostrea gigas

characteristic: organism

characteristic: pcr conditions

text: f: linker (3bp) + 16S_338r: CATGCTGCCTCCCGTAGGAGT; r: linker (2bp) + 16S_27F: TCAGAGTTTGATCMTGGCTCAG

characteristic: pcr primers

text: temperature stressed, mock-infected

characteristic: perturbation

characteristic: project name

text: syringe

characteristic: sample collection device or method

text: Crassostrea gigas

text: pyrosequencing

characteristic: substrate

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: temperature

text: C.gigas hemolymph microbiota CW_0_5


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: f: adapter A + key (4bp): CGTATCGCCTCCCTCGCGCCATCAG; r: adapter B + key (4bp): CTATGCGCCTTGCCAGCCCGCTCAG

characteristic: adapters

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

characteristic: body site

characteristic: collection date

text: Pacific oyster

characteristic: common name

characteristic: description

text: 7 days after transfer from flowthrough to stationary seawater aquarium (both 22°C) and injection with soya-peptone medium

characteristic: detailed conditions

text: healthy

characteristic: disease status

text: aquatic, marine

characteristic: environment (biome)

text: organic

characteristic: environment (feature)

text: organic material, bodily fluid

characteristic: environment (material)

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host body temperature

text: Pacific oyster

text: host-associated

text: mimarks-survey

characteristic: investigation type

text: adult

text: f: AGTGTATGTC; r: AGACGCACTC or f: AGTGTATGTC; r: ATATCGCGAG

characteristic: multiplex identifiers

text: Crassostrea gigas

text: temperature acclimated, mock-infected

text: syringe

text: Crassostrea gigas

text: pyrosequencing

characteristic: sequencing method

text: seawater (local, supplied through cca 200 m long pipe, 30 um filtered and protein skimmed)

text: 16S rRNA

text: C.gigas hemolymph microbiota WW_0_7

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

text: Pacific oyster

text: Experimental sample. Sample title code: [original temperature][experimental temperature]_[infection]_[time point]. For details see study description and 'perturbation' and 'detailed conditions' fields

text: held in flowthrough aquarium, 8°C, well acclimated

text: healthy

text: aquatic, marine

text: organic

text: organic material, bodily fluid

text: Germany

text: Pacific oyster

characteristic: host common name

characteristic: host subject id

characteristic: host taxid

text: host-associated

characteristic: host-associated environmental package

text: mimarks-survey

text: adult

characteristic: life stage

characteristic: location details

text: f: ACAGTATATA; r: ACGCTCGACA or f: ACAGTATATA; r: AGCACTGTAG

text: Crassostrea gigas

characteristic: organism

characteristic: pcr conditions

characteristic: pcr primers

text: temperature acclimated

characteristic: perturbation

characteristic: project name

text: syringe

characteristic: sample collection device or method

text: Crassostrea gigas

text: pyrosequencing

characteristic: substrate

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: temperature

text: C.gigas hemolymph microbiota CW_0_0


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

characteristic: body site

characteristic: collection date

text: Pacific oyster

characteristic: common name

characteristic: description

text: 3 days after transfer from flowthrough to stationary seawater aquarium (both 22°C) and injection with Vibrio sp. D29w strain

characteristic: detailed conditions

text: healthy

characteristic: disease status

text: aquatic, marine

characteristic: environment (biome)

text: organic

characteristic: environment (feature)

text: organic material, bodily fluid

characteristic: environment (material)

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host body temperature

text: Pacific oyster

text: host-associated

text: mimarks-survey

characteristic: investigation type

text: adult

text: Schleswig-Holstein, Wadden Sea (North Sea), island of Sylt, Kongishafen

text: f: ACTACTATGT; r: AGACGCACTC or f: ACTACTATGT; r: ATATCGCGAG

characteristic: multiplex identifiers

text: Crassostrea gigas

text: temperature acclimated, infected

text: syringe

text: Crassostrea gigas

text: pyrosequencing

characteristic: sequencing method

text: 16S rRNA

text: C.gigas hemolymph microbiota WW_1_3

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

text: Pacific oyster

text: 7 days after transfer from flowthrough to stationary seawater aquarium, temperature change from 8°C to 22°C and injection with soya-peptone medium

text: healthy

text: aquatic, marine

text: organic

text: organic material, bodily fluid

text: Germany

text: Pacific oyster

characteristic: host common name

characteristic: host subject id

characteristic: host taxid

text: host-associated

characteristic: host-associated environmental package

text: mimarks-survey

text: adult

characteristic: life stage

characteristic: location details

text: f: ATCTACTGAC; r: AGACGCACTC or f: ATCTACTGAC; r: ATATCGCGAG

text: Crassostrea gigas

characteristic: organism

characteristic: pcr conditions

characteristic: pcr primers

text: temperature stressed, mock-infected

characteristic: perturbation

characteristic: project name

text: syringe

characteristic: sample collection device or method

text: Crassostrea gigas

text: pyrosequencing

characteristic: substrate

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: temperature

text: C.gigas hemolymph microbiota CW_0_7


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

characteristic: body site

characteristic: collection date

text: Pacific oyster

characteristic: common name

characteristic: description

text: 7 days after transfer from flowthrough to stationary seawater aquarium, temperature change from 22°C to 8°C and injection with soya-peptone medium

characteristic: detailed conditions

text: healthy

characteristic: disease status

text: aquatic, marine

characteristic: environment (biome)

text: organic

characteristic: environment (feature)

text: organic material, bodily fluid

characteristic: environment (material)

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host body temperature

text: Pacific oyster

text: host-associated

text: mimarks-survey

characteristic: investigation type

text: adult

text: f: AGTATACATA; r: AGACGCACTC or f: AGTATACATA; r: ATATCGCGAG

characteristic: multiplex identifiers

text: Crassostrea gigas

text: initial denaturation:94degC_1min; denaturation:94degC_40s;annealing:55degC_30s; elongation:72degC_30s; final elongation:72degC_2min; 35 cycles

text: temperature stressed, mock-infected

text: syringe

text: Crassostrea gigas

text: pyrosequencing

characteristic: sequencing method

text: 16S rRNA

text: C.gigas hemolymph microbiota WC_0_7

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ALFRED WEGENER INSTITUTE FOR POLAR AND MARINE RESE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

text: Pacific oyster

text: held in flowthrough aquarium, 22°C, well acclimated

text: healthy

text: aquatic, marine

text: organic

text: organic material, bodily fluid

text: Germany

text: Pacific oyster

characteristic: host common name

characteristic: host subject id

characteristic: host taxid

text: host-associated

characteristic: host-associated environmental package

text: mimarks-survey

text: adult

characteristic: life stage

characteristic: location details

text: f: AGTACGCTAT; r: ACGCTCGACA or f: AGTACGCTAT; r: AGCACTGTAG

text: Crassostrea gigas

characteristic: organism

characteristic: pcr conditions

characteristic: pcr primers

text: temperature acclimated

characteristic: perturbation

characteristic: project name

text: syringe

characteristic: sample collection device or method

text: Crassostrea gigas

text: pyrosequencing

characteristic: substrate

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: temperature

text: C.gigas hemolymph microbiota WW_0_0


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: f: adapter A + key (4bp): CGTATCGCCTCCCTCGCGCCATCAG; r: adapter B + key (4bp): CTATGCGCCTTGCCAGCCCGCTCAG

characteristic: adapters

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

characteristic: body site

characteristic: collection date

text: Pacific oyster

characteristic: common name

characteristic: description

text: 3 days after transfer from flowthrough to stationary seawater aquarium, temperature change from 8°C to 22°C and injection with Vibrio sp. D29w strain

characteristic: detailed conditions

text: dead

characteristic: disease status

text: aquatic, marine

characteristic: environment (biome)

text: organic

characteristic: environment (feature)

text: organic material, bodily fluid

characteristic: environment (material)

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host body temperature

text: Pacific oyster

text: host-associated

text: mimarks-survey

characteristic: investigation type

text: adult

text: f: TCTATACTAT; r: AGACGCACTC or f: TCTATACTAT; r: ATATCGCGAG

characteristic: multiplex identifiers

text: Crassostrea gigas

text: f: linker (3bp) + 16S_338r: CATGCTGCCTCCCGTAGGAGT; r: linker (2bp) + 16S_27F: TCAGAGTTTGATCMTGGCTCAG

text: temperature stressed, infected

text: syringe

text: Crassostrea gigas

text: pyrosequencing

characteristic: sequencing method

text: 16S rRNA

text: C.gigas hemolymph microbiota CW_1_3

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

text: Pacific oyster

text: Experimental sample. Sample title code: [original temperature][experimental temperature]_[infection]_[time point]. For details see study description and 'perturbation' and 'detailed conditions' fields

text: 7 days after transfer from flowthrough to stationary seawater aquarium, temperature change from 22°C to 8°C and injection with Vibrio sp. D29w strain

text: healthy

text: aquatic, marine

text: organic

text: organic material, bodily fluid

text: Germany

text: Pacific oyster

characteristic: host common name

characteristic: host subject id

characteristic: host taxid

text: host-associated

characteristic: host-associated environmental package

text: mimarks-survey

text: adult

characteristic: life stage

characteristic: location details

text: f: TGTAGTGTGA; r: AGACGCACTC or f: TGTAGTGTGA; r: ATATCGCGAG

text: Crassostrea gigas

characteristic: organism

characteristic: pcr conditions

characteristic: pcr primers

text: temperature stressed, infected

characteristic: perturbation

characteristic: project name

text: syringe

characteristic: sample collection device or method

text: Crassostrea gigas

text: pyrosequencing

text: seawater (local, supplied through cca 200 m long pipe, 30 um filtered and protein skimmed)

characteristic: substrate

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: temperature

text: C.gigas hemolymph microbiota WC_1_7


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

characteristic: body site

characteristic: collection date

text: Pacific oyster

characteristic: common name

characteristic: description

characteristic: detailed conditions

text: healthy

characteristic: disease status

text: aquatic, marine

characteristic: environment (biome)

text: organic

characteristic: environment (feature)

text: organic material, bodily fluid

characteristic: environment (material)

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host body temperature

text: Pacific oyster

text: host-associated

text: mimarks-survey

characteristic: investigation type

text: adult

text: Schleswig-Holstein, Wadden Sea (North Sea), island of Sylt, Kongishafen

text: f: ACAGTCGTGC; r: AGACGCACTC or f: ACAGTCGTGC; r: ATATCGCGAG

characteristic: multiplex identifiers

text: Crassostrea gigas

text: temperature stressed, mock-infected

text: syringe

text: Crassostrea gigas

text: pyrosequencing

characteristic: sequencing method

text: 16S rRNA

text: C.gigas hemolymph microbiota CW_0_7

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

text: Pacific oyster

text: 3 days after transfer from flowthrough to stationary seawater aquarium (both 8°C) and injection with Vibrio sp. D29w strain

text: healthy

text: aquatic, marine

text: organic

text: organic material, bodily fluid

text: Germany

text: Pacific oyster

characteristic: host common name

characteristic: host subject id

characteristic: host taxid

text: host-associated

characteristic: host-associated environmental package

text: mimarks-survey

text: adult

characteristic: life stage

characteristic: location details

text: f: ATACGACGTA; r: AGACGCACTC or f: ATACGACGTA; r: ATATCGCGAG

text: Crassostrea gigas

characteristic: organism

characteristic: pcr conditions

characteristic: pcr primers

text: temperature acclimated, infected

characteristic: perturbation

characteristic: project name

text: syringe

characteristic: sample collection device or method

text: Crassostrea gigas

text: pyrosequencing

characteristic: substrate

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: temperature

text: C.gigas hemolymph microbiota CC_1_3


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

characteristic: body site

characteristic: collection date

text: Pacific oyster

characteristic: common name

characteristic: description

text: held in flowthrough aquarium, 8°C, well acclimated

characteristic: detailed conditions

text: healthy

characteristic: disease status

text: aquatic, marine

characteristic: environment (biome)

text: organic

characteristic: environment (feature)

text: organic material, bodily fluid

characteristic: environment (material)

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host body temperature

text: Pacific oyster

text: host-associated

text: mimarks-survey

characteristic: investigation type

text: adult

text: f: ATATCGCGAG; r: ACGCTCGACA or f: ATATCGCGAG; r: AGCACTGTAG

characteristic: multiplex identifiers

text: Crassostrea gigas

text: temperature acclimated

text: syringe

text: Crassostrea gigas

text: pyrosequencing

characteristic: sequencing method

text: 16S rRNA

text: C.gigas hemolymph microbiota CC_0_0

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ALFRED WEGENER INSTITUTE FOR POLAR AND MARINE RESE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

text: Pacific oyster

text: 5 days after transfer from flowthrough to stationary seawater aquarium, temperature change from 8°C to 22°C and injection with soya-peptone medium

text: healthy

text: aquatic, marine

text: organic

text: organic material, bodily fluid

text: Germany

text: Pacific oyster

characteristic: host common name

characteristic: host subject id

characteristic: host taxid

text: host-associated

characteristic: host-associated environmental package

text: mimarks-survey

text: adult

characteristic: life stage

characteristic: location details

text: f: CACGTAGATC; r: ACGCTCGACA or f: CACGTAGATC; r: AGCACTGTAG

text: Crassostrea gigas

characteristic: organism

text: initial denaturation:94degC_1min; denaturation:94degC_40s;annealing:55degC_30s; elongation:72degC_30s; final elongation:72degC_2min; 35 cycles

characteristic: pcr conditions

characteristic: pcr primers

text: temperature stressed, mock-infected

characteristic: perturbation

characteristic: project name

text: syringe

characteristic: sample collection device or method

text: Crassostrea gigas

text: pyrosequencing

characteristic: substrate

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: temperature

text: C.gigas hemolymph microbiota CW_0_5


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: f: adapter A + key (4bp): CGTATCGCCTCCCTCGCGCCATCAG; r: adapter B + key (4bp): CTATGCGCCTTGCCAGCCCGCTCAG

characteristic: adapters

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

characteristic: body site

characteristic: collection date

text: Pacific oyster

characteristic: common name

characteristic: description

text: 7 days after transfer from flowthrough to stationary seawater aquarium (both 8°C) and injection with soya-peptone medium

characteristic: detailed conditions

text: healthy

characteristic: disease status

text: aquatic, marine

characteristic: environment (biome)

text: organic

characteristic: environment (feature)

text: organic material, bodily fluid

characteristic: environment (material)

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host body temperature

text: Pacific oyster

text: host-associated

text: mimarks-survey

characteristic: investigation type

text: adult

text: f: CTATAGCGTA; r: AGACGCACTC or f: CTATAGCGTA; r: ATATCGCGAG

characteristic: multiplex identifiers

text: Crassostrea gigas

text: temperature acclimated, mock-infected

text: syringe

text: Crassostrea gigas

text: pyrosequencing

characteristic: sequencing method

text: 16S rRNA

text: C.gigas hemolymph microbiota CC_0_7

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

text: Pacific oyster

text: Experimental sample. Sample title code: [original temperature][experimental temperature]_[infection]_[time point]. For details see study description and 'perturbation' and 'detailed conditions' fields

text: 3 days after transfer from flowthrough to stationary seawater aquarium (both 8°C) and injection with soya-peptone medium

text: healthy

text: aquatic, marine

text: organic

text: organic material, bodily fluid

text: Germany

text: Pacific oyster

characteristic: host common name

characteristic: host subject id

characteristic: host taxid

text: host-associated

characteristic: host-associated environmental package

text: mimarks-survey

text: adult

characteristic: life stage

characteristic: location details

text: f: CGTGTCTCTA; r: AGACGCACTC or f: CGTGTCTCTA; r: ATATCGCGAG

text: Crassostrea gigas

characteristic: organism

characteristic: pcr conditions

text: f: linker (3bp) + 16S_338r: CATGCTGCCTCCCGTAGGAGT; r: linker (2bp) + 16S_27F: TCAGAGTTTGATCMTGGCTCAG

characteristic: pcr primers

text: temperature acclimated, mock-infected

characteristic: perturbation

characteristic: project name

text: syringe

characteristic: sample collection device or method

text: Crassostrea gigas

text: pyrosequencing

characteristic: substrate

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: temperature

text: C.gigas hemolymph microbiota CC_0_3


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

characteristic: body site

characteristic: collection date

text: Pacific oyster

characteristic: common name

characteristic: description

text: 5 days after transfer from flowthrough to stationary seawater aquarium (both 8°C) and injection with Vibrio sp. D29w strain

characteristic: detailed conditions

text: healthy

characteristic: disease status

text: aquatic, marine

characteristic: environment (biome)

text: organic

characteristic: environment (feature)

text: organic material, bodily fluid

characteristic: environment (material)

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host body temperature

text: Pacific oyster

text: host-associated

text: mimarks-survey

characteristic: investigation type

text: adult

text: Schleswig-Holstein, Wadden Sea (North Sea), island of Sylt, Kongishafen

text: f: TCGATAGTGA; r: ACGCTCGACA or f: TCGATAGTGA; r: AGCACTGTAG

characteristic: multiplex identifiers

text: Crassostrea gigas

text: temperature acclimated, infected

text: syringe

text: Crassostrea gigas

text: pyrosequencing

characteristic: sequencing method

text: seawater (local, supplied through cca 200 m long pipe, 30 um filtered and protein skimmed)

text: 16S rRNA

text: C.gigas hemolymph microbiota CC_1_5

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

text: Pacific oyster

text: 5 days after transfer from flowthrough to stationary seawater aquarium, temperature change from 22°C to 8°C and injection with soya-peptone medium

text: healthy

text: aquatic, marine

text: organic

text: organic material, bodily fluid

text: Germany

text: Pacific oyster

characteristic: host common name

characteristic: host subject id

characteristic: host taxid

text: host-associated

characteristic: host-associated environmental package

text: mimarks-survey

text: adult

characteristic: life stage

characteristic: location details

text: f: AGTATACATA; r: ACGCTCGACA or f: AGTATACATA; r: AGCACTGTAG

text: Crassostrea gigas

characteristic: organism

characteristic: pcr conditions

characteristic: pcr primers

text: temperature stressed, mock-infected

characteristic: perturbation

characteristic: project name

text: syringe

characteristic: sample collection device or method

text: Crassostrea gigas

text: pyrosequencing

characteristic: substrate

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: temperature

text: C.gigas hemolymph microbiota WC_0_5


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ALFRED WEGENER INSTITUTE FOR POLAR AND MARINE RESE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

characteristic: body site

characteristic: collection date

text: Pacific oyster

characteristic: common name

characteristic: description

text: held in flowthrough aquarium, 22°C, well acclimated

characteristic: detailed conditions

text: healthy

characteristic: disease status

text: aquatic, marine

characteristic: environment (biome)

text: organic

characteristic: environment (feature)

text: organic material, bodily fluid

characteristic: environment (material)

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host body temperature

text: Pacific oyster

text: host-associated

text: mimarks-survey

characteristic: investigation type

text: adult

text: f: TCTCTATGCG; r: ACGCTCGACA or f: TCTCTATGCG; r: AGCACTGTAG

characteristic: multiplex identifiers

text: Crassostrea gigas

text: temperature acclimated

text: syringe

text: Crassostrea gigas

text: pyrosequencing

characteristic: sequencing method

text: 16S rRNA

text: C.gigas hemolymph microbiota WC_0_0

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

text: Pacific oyster

text: 7 days after transfer from flowthrough to stationary seawater aquarium, temperature change from 8°C to 22°C and injection with soya-peptone medium

text: healthy

text: aquatic, marine

text: organic

text: organic material, bodily fluid

text: Germany

text: Pacific oyster

characteristic: host common name

characteristic: host subject id

characteristic: host taxid

text: host-associated

characteristic: host-associated environmental package

text: mimarks-survey

text: adult

characteristic: life stage

characteristic: location details

text: f: ACGTCTCATC; r: AGACGCACTC or f: ACGTCTCATC; r: ATATCGCGAG

text: Crassostrea gigas

characteristic: organism

text: initial denaturation:94degC_1min; denaturation:94degC_40s;annealing:55degC_30s; elongation:72degC_30s; final elongation:72degC_2min; 35 cycles

characteristic: pcr conditions

characteristic: pcr primers

text: temperature stressed, mock-infected

characteristic: perturbation

characteristic: project name

text: syringe

characteristic: sample collection device or method

text: Crassostrea gigas

text: pyrosequencing

characteristic: substrate

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: temperature

text: C.gigas hemolymph microbiota CW_0_7


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: f: adapter A + key (4bp): CGTATCGCCTCCCTCGCGCCATCAG; r: adapter B + key (4bp): CTATGCGCCTTGCCAGCCCGCTCAG

characteristic: adapters

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

characteristic: body site

characteristic: collection date

text: Pacific oyster

characteristic: common name

characteristic: description

text: 7 days after transfer from flowthrough to stationary seawater aquarium, temperature change from 22°C to 8°C and injection with soya-peptone medium

characteristic: detailed conditions

text: healthy

characteristic: disease status

text: aquatic, marine

characteristic: environment (biome)

text: organic

characteristic: environment (feature)

text: organic material, bodily fluid

characteristic: environment (material)

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host body temperature

text: Pacific oyster

text: host-associated

text: mimarks-survey

characteristic: investigation type

text: adult

text: f: CGATCGTATA; r: AGACGCACTC or f: CGATCGTATA; r: ATATCGCGAG

characteristic: multiplex identifiers

text: Crassostrea gigas

text: temperature stressed, mock-infected

text: syringe

text: Crassostrea gigas

text: pyrosequencing

characteristic: sequencing method

text: 16S rRNA

text: C.gigas hemolymph microbiota WC_0_7

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

text: Pacific oyster

text: Experimental sample. Sample title code: [original temperature][experimental temperature]_[infection]_[time point]. For details see study description and 'perturbation' and 'detailed conditions' fields

text: 3 days after transfer from flowthrough to stationary seawater aquarium, temperature change from 22°C to 8°C and injection with soya-peptone medium

text: healthy

text: aquatic, marine

text: organic

text: organic material, bodily fluid

text: Germany

text: Pacific oyster

characteristic: host common name

characteristic: host subject id

characteristic: host taxid

text: host-associated

characteristic: host-associated environmental package

text: mimarks-survey

text: adult

characteristic: life stage

characteristic: location details

text: f: TCGTCGCTCG; r: AGACGCACTC or f: TCGTCGCTCG; r: ATATCGCGAG

text: Crassostrea gigas

characteristic: organism

characteristic: pcr conditions

characteristic: pcr primers

text: temperature stressed, mock-infected

characteristic: perturbation

characteristic: project name

text: syringe

characteristic: sample collection device or method

text: Crassostrea gigas

text: pyrosequencing

characteristic: substrate

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: temperature

text: C.gigas hemolymph microbiota WC_0_3


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

characteristic: body site

characteristic: collection date

text: Pacific oyster

characteristic: common name

characteristic: description

text: held in flowthrough aquarium, 8°C, well acclimated

characteristic: detailed conditions

text: healthy

characteristic: disease status

text: aquatic, marine

characteristic: environment (biome)

text: organic

characteristic: environment (feature)

text: organic material, bodily fluid

characteristic: environment (material)

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host body temperature

text: Pacific oyster

text: host-associated

text: mimarks-survey

characteristic: investigation type

text: adult

text: Schleswig-Holstein, Wadden Sea (North Sea), island of Sylt, Kongishafen

text: f: TAGAGACGAG; r: ACGCTCGACA or f: TAGAGACGAG; r: AGCACTGTAG

characteristic: multiplex identifiers

text: Crassostrea gigas

text: f: linker (3bp) + 16S_338r: CATGCTGCCTCCCGTAGGAGT; r: linker (2bp) + 16S_27F: TCAGAGTTTGATCMTGGCTCAG

text: temperature acclimated

text: syringe

text: Crassostrea gigas

text: pyrosequencing

characteristic: sequencing method

text: 16S rRNA

text: C.gigas hemolymph microbiota CC_1_0

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

text: Pacific oyster

text: healthy

text: aquatic, marine

text: organic

text: organic material, bodily fluid

text: Germany

text: Pacific oyster

characteristic: host common name

characteristic: host subject id

characteristic: host taxid

text: host-associated

characteristic: host-associated environmental package

text: mimarks-survey

text: adult

characteristic: life stage

characteristic: location details

text: f: TCGCACTAGT; r: ACGCTCGACA or f: TCGCACTAGT; r: AGCACTGTAG

text: Crassostrea gigas

characteristic: organism

characteristic: pcr conditions

characteristic: pcr primers

text: temperature acclimated

characteristic: perturbation

characteristic: project name

text: syringe

characteristic: sample collection device or method

text: Crassostrea gigas

text: pyrosequencing

text: seawater (local, supplied through cca 200 m long pipe, 30 um filtered and protein skimmed)

characteristic: substrate

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: temperature

text: C.gigas hemolymph microbiota CW_0_0


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ALFRED WEGENER INSTITUTE FOR POLAR AND MARINE RESE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

characteristic: body site

characteristic: collection date

text: Pacific oyster

characteristic: common name

characteristic: description

text: 3 days after transfer from flowthrough to stationary seawater aquarium (both 22°C) and injection with Vibrio sp. D29w strain

characteristic: detailed conditions

text: healthy

characteristic: disease status

text: aquatic, marine

characteristic: environment (biome)

text: organic

characteristic: environment (feature)

text: organic material, bodily fluid

characteristic: environment (material)

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host body temperature

text: Pacific oyster

text: host-associated

text: mimarks-survey

characteristic: investigation type

text: adult

text: f: AGCGTCGTCT; r: AGACGCACTC or f: AGCGTCGTCT; r: ATATCGCGAG

characteristic: multiplex identifiers

text: Crassostrea gigas

text: temperature acclimated, infected

text: syringe

text: Crassostrea gigas

text: pyrosequencing

characteristic: sequencing method

text: 16S rRNA

text: C.gigas hemolymph microbiota WW_1_3

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

text: Pacific oyster

text: 7 days after transfer from flowthrough to stationary seawater aquarium (both 22°C) and injection with soya-peptone medium

text: healthy

text: aquatic, marine

text: organic

text: organic material, bodily fluid

text: Germany

text: Pacific oyster

characteristic: host common name

characteristic: host subject id

characteristic: host taxid

text: host-associated

characteristic: host-associated environmental package

text: mimarks-survey

text: adult

characteristic: life stage

characteristic: location details

text: f: ACTCGCGCAC; r: AGACGCACTC or f: ACTCGCGCAC; r: ATATCGCGAG

text: Crassostrea gigas

characteristic: organism

characteristic: pcr conditions

characteristic: pcr primers

text: temperature acclimated, mock-infected

characteristic: perturbation

characteristic: project name

text: syringe

characteristic: sample collection device or method

text: Crassostrea gigas

text: pyrosequencing

characteristic: substrate

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: temperature

text: C.gigas hemolymph microbiota WW_0_7


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: f: adapter A + key (4bp): CGTATCGCCTCCCTCGCGCCATCAG; r: adapter B + key (4bp): CTATGCGCCTTGCCAGCCCGCTCAG

characteristic: adapters

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

characteristic: body site

characteristic: collection date

text: Pacific oyster

characteristic: common name

characteristic: description

characteristic: detailed conditions

text: healthy

characteristic: disease status

text: aquatic, marine

characteristic: environment (biome)

text: organic

characteristic: environment (feature)

text: organic material, bodily fluid

characteristic: environment (material)

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host body temperature

text: Pacific oyster

text: host-associated

text: mimarks-survey

characteristic: investigation type

text: adult

text: f: ATCAGACACG; r: AGACGCACTC or f: ATCAGACACG; r: ATATCGCGAG

characteristic: multiplex identifiers

text: Crassostrea gigas

text: initial denaturation:94degC_1min; denaturation:94degC_40s;annealing:55degC_30s; elongation:72degC_30s; final elongation:72degC_2min; 35 cycles

text: temperature stressed, mock-infected

text: syringe

text: Crassostrea gigas

text: pyrosequencing

characteristic: sequencing method

text: 16S rRNA

text: C.gigas hemolymph microbiota WC_0_3

characteristic: title