characteristic: Chlorophyll Sensor

characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: GSC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (TARA_N010000330) was collected at station TARA_175 during campaign TARA_20130629Z of the Tara Oceans expedition (2009-2013), using a Rosette Vertical Sampling System [RVSS]. The sampling event (TARA_20130711T0613Z_175_Combined-EVENTS_CAST) occurred at position latitudeN=79.3349 and longitudeE=66.2954, on date/time=2013-07-11T06:13:00, at a depth of 10-100 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [MIX] marine epipelagic wind mixed layer (ENVO:01000061). The sample material was size-fractionated (>0.8 micrometres) and prepared on board using protocol [SEQ-(100L-or-15min)_W>0.8] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity, metagenomics and/or transcriptomics studies of unicellular eukaryotes (protists). This sample has replicate sample(s): TARA_N010000331, TARA_N010000574, TARA_S100001505, TARA_N010000575, TARA_N010000328, TARA_N010000329. The sample material was split into several aliquot samples including this one and sample(s): TARA_N010000331.

characteristic: description

text: Polar Biome

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (feature) further details

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

characteristic: event label

characteristic: further details

characteristic: last update date

characteristic: latitude start

text: [AO] Arctic Ocean (MRGID:1906)

text: marine metagenome

characteristic: organism

text: Tara Oceans Expedition (2009-2013)

text: In the size fraction W>0.8 micrometres, the sample material was concentrated on 0.8-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane filter (ref:ATTP14250) on top of a Dacron pad mesh. A volume of 100 Litres was filtered in parallel on two 0.8-micrometre-pore-size filters. Each filter was then packaged in a sterile cryogenic tube (5 mL) (2 tubes, each with 1 filter), labelled with a barcode identification sticker and stored on board in liquid nitrogen. Note 1: Latex or nitril gloves were used for this protocol. Note 2: All containers, filter holders and tubing were washed with 0.1% bleach, rinsed twice with fresh water and rinsed again twice with the pre-filtered sample material. All tweezers are kept clean with ethanol. Note 3: The entire process did not exceed 15 minutes. Note 4: The total volume filtered on the two filters is 100 Litres. However, the volume filtered on each filter is unknown, but approximately 50 Litres. The intention of this protocol is to combine the two filters during molecular entities (nucleotides) extraction and sequencing analysis. A total of 2 (2x 2 tubes) replicates were prepared and sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

text: Rosette Vertical Sampling System [RVSS]

text: SV Tara

text: marine metagenome

text: TARA_20130711T0613Z_175_Combined-EVENTS_CAST_MIX_(10-100m)_SEQ-(100L-or-15min)_W>0.8_N010000330

characteristic: title


status: PUBLIC

unit: mg Chl/m3

tag: attribute

characteristic: Chlorophyll Sensor

text: Tara Oceans Consortium, Coordinators & Tara Oceans Expedition, Participants. Registry of all samples from the Tara Oceans Expedition (2009-2013). PANGAEA. doi:10.1594/PANGAEA.842197 (2015) (https://dx.doi.org/10.1594/PANGAEA.842197)

characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: GSC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

unit: DD

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

unit: practical salinity unit

unit: m

text: This sample (TARA_N010000334) was collected at station TARA_175 during campaign TARA_20130629Z of the Tara Oceans expedition (2009-2013), using a Rosette Vertical Sampling System [RVSS]. The sampling event (TARA_20130711T0613Z_175_Combined-EVENTS_CAST) occurred at position latitudeN=79.3349 and longitudeE=66.2954, on date/time=2013-07-11T06:13:00, at a depth of 10-100 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [MIX] marine epipelagic wind mixed layer (ENVO:01000061). The sample material was size-fractionated (3-20 micrometres) and prepared on board using protocol [SEQ-(10-100L-or-15min)_W3-20] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity, metagenomics and/or transcriptomics studies of unicellular eukaryotes (protists) and attached prokaryotes. This sample has replicate sample(s): TARA_N010000335, TARA_N010000573, TARA_N010000333, TARA_N010000332.

text: Polar Biome

text: depth interval within the epipelagic zone (ENVO:00000209) including part of the photic zone (ENVO:00000209) and part of the aphotic zone (ENVO:00000210), including a strong peak in chlorophyll fluorescence, within a marine epipelagic wind mixed layer (ENVO:01000061)

text: water (ENVO:00002006), including particulate matter (ENVO:01000060) such as planktonic material (ENVO:01000063)

text: water

characteristic: longitude start

text: [AO] Arctic Ocean (MRGID:1906)

characteristic: marine region

unit: µmol/L

characteristic: nitrate sensor

text: marine metagenome

unit: µmol/kg

characteristic: oxygen sensor

text: Tara Oceans Expedition (2009-2013)

characteristic: project name

text: In the size fraction W3-20 micrometres, the sample material was pre-filtered on 20-micrometres, using a nylon mesh, and concentrated on 3-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane filter (ref:TSTP14250). A volume of 10-100 Litres was filtered and the 3-micrometre-pore-size filter was packaged in a sterile cryogenic tube (5 mL), labelled with a barcode identification sticker and stored on board in liquid nitrogen. Note 1: Latex or nitril gloves were used for this protocol. Note 2: All containers, filter holders and tubing were washed with 0.1% bleach, rinsed twice with fresh water and rinsed again twice with the pre-filtered sample material. All tweezers are kept clean with ethanol. Note 3: The entire process did not exceed 15 minutes. Note 4: The volume filtered is indicated for each replicate in the Sample registry (http://doi.pangaea.de/10.1594/PANGAEA.842197). A total of 2-6 replicates were prepared and sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

characteristic: protocol description

characteristic: protocol label

text: Rosette Vertical Sampling System [RVSS]

characteristic: sample collection device

characteristic: sample material label

text: This version can be used to provide data discovery services

characteristic: sample status

characteristic: sampling campaign

text: SV Tara

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

unit: ºC

characteristic: temperature

text: TARA_20130711T0613Z_175_Combined-EVENTS_CAST_MIX_(10-100m)_SEQ-(10-100L-or-15min)_W3-20_N010000334


characteristic: Chlorophyll Sensor

characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: GSC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (TARA_B110000122) was collected at station TARA_175 during campaign TARA_20130629Z of the Tara Oceans expedition (2009-2013), using a Rosette Vertical Sampling System [RVSS]. The sampling event (TARA_20130711T0613Z_175_Combined-EVENTS_CAST) occurred at position latitudeN=79.3349 and longitudeE=66.2954, on date/time=2013-07-11T06:13:00, at a depth of 10-100 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [MIX] marine epipelagic wind mixed layer (ENVO:01000061). The sample material was size-fractionated (0.22-3 micrometres) and prepared on board using protocol [SEQ-(10L-or-15min)_W0.22-3] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity, metagenomics and/or transcriptomics studies of prokaryotes. This sample has replicate sample(s): TARA_B110000123.

characteristic: description

text: Polar Biome

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (feature) further details

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

characteristic: event label

characteristic: further details

characteristic: last update date

characteristic: latitude start

text: [AO] Arctic Ocean (MRGID:1906)

text: marine metagenome

characteristic: organism

text: Tara Oceans Expedition (2009-2013)

text: In the size fraction W0.22-3 micrometres, the sample material was pre-filtered on 3-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane (ref:TSTP14250), and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 10 Litres was filtered and the 0.22-micrometre-pore-size filter was packaged in a sterile Falcon tube (50 mL), treated with the addition of 10 mL of RNA-Later, labelled with a barcode identification sticker and stored on board in a freezer at -20 degree Celsius. Note 1: Latex or nitril gloves were used for this protocol. Note 2: All containers, filter holders and tubing were washed with 0.1% bleach, rinsed twice with fresh water and rinsed again twice with the pre-filtered sample material. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump (Masterflex, EW-77410-10) was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The entire process did not exceed 15 minutes. A total of 2 replicates were prepared and sent for later analysis to the Institute of Marine Science (CSIC), Barcelona, Spain.

text: Rosette Vertical Sampling System [RVSS]

text: SV Tara

text: marine metagenome

text: TARA_20130711T0613Z_175_Combined-EVENTS_CAST_MIX_(10-100m)_SEQ-(10L-or-15min)_W0.22-3_B110000122

characteristic: title


characteristic: Chlorophyll Sensor

characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: GSC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

text: This sample (TARA_R110002094) was collected at station TARA_175 during campaign TARA_20130629Z of the Tara Oceans expedition (2009-2013), using a Rosette Vertical Sampling System [RVSS]. The sampling event (TARA_20130711T0613Z_175_Combined-EVENTS_CAST) occurred at position latitudeN=79.3349 and longitudeE=66.2954, on date/time=2013-07-11T06:13:00, at a depth of 10-100 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [MIX] marine epipelagic wind mixed layer (ENVO:01000061). The sample material was size-fractionated (<0.22 micrometres) and prepared on board using protocol [SEQ-(FeCl3)_W<0.22] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of viruses. This sample has no known replicate.

text: Polar Biome

text: water

characteristic: longitude start

text: [AO] Arctic Ocean (MRGID:1906)

characteristic: marine region

characteristic: nitrate sensor

text: marine metagenome

characteristic: oxygen sensor

text: Tara Oceans Expedition (2009-2013)

characteristic: project name

text: In the size fraction W<0.22 micrometres, the sample material was pre-filtered using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane (ref:GPWP14250) with 0.22-micrometres-pore-size. A volume of 20 Litres was treated with FeCl3, incubated at room temperature for 1-24 hours to allow viruses to precipitate, filtered onto a 1-micrometre-pore-size, 142-mm-diameter GE polycarbonate (PC) membrane filter (ref:K10CP14220) placed on top of a 0.8-micrometre-pore-size, 142-mm-diameter Pall Gelman support filter (ref:60114) using a peristaltic pump with pressure <15 psi. The 1-micrometre-pore-size filter was then packaged in a sterile Falcon tube (50 mL) (up to 3 filters in 1 tube), labelled with a barcode identification sticker and stored on board in a refrigerator at +4 degree Celsius. Note 1: Latex or nitril gloves were used for this protocol. Note 2: All containers, filter holders and tubing were washed with 0.1% bleach, rinsed twice with fresh water and rinsed again twice with the pre-filtered sample material. All tweezers are kept clean with ethanol. Note 3: The three replicates were incubated together. For 20 Litres of filtrate, 2 millilitres of FeCl3 stock solution (4.83 grams of FeCl3-6H2O + 100 millilitres of MilliQ water, stored at 4°C) was added (final concentration of 1 mg Fe per Litre of filtrate), and shaken vigorously for 1 minute. Note 4: Further details about the method are published here doi:10.1111/j.1758-2229.2010.00208.x. In 2009-2012, a total of 3 replicates were prepared and sent for later analysis to the University of Arizona (UA), Tucson, USA. In 2013, a total of 2 replicates were prepared, one sent to for later analysis to the University of Arizona (UA), Tucson, USA, and the other sent for later analysis to the Observatoire Océanologique de Banyuls sur Mer (OOB), France.

characteristic: protocol description

characteristic: protocol label

text: Rosette Vertical Sampling System [RVSS]

characteristic: sample collection device

characteristic: sample material label

characteristic: sample status

characteristic: sampling campaign

text: SV Tara

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: TARA_20130711T0613Z_175_Combined-EVENTS_CAST_MIX_(10-100m)_SEQ-(FeCl3)_W<0.22_R110002094


characteristic: Chlorophyll Sensor

characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: GSC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (TARA_N010000338) was collected at station TARA_175 during campaign TARA_20130629Z of the Tara Oceans expedition (2009-2013), using a plankton net [NET-BONGO-180]+[SIEVE-2000]. The sampling event (TARA_20130711T1225Z_175_EVENT_NET) occurred at position latitudeN=79.4865 and longitudeE=66.1758, on date/time=2013-07-11T12:25:00, at a depth of 0-100 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [MIX] marine epipelagic wind mixed layer (ENVO:01000061). The sample material was size-fractionated (180-2000 micrometres) and prepared on board using protocol [SEQ-(500mL-or-15min)_N180-2000] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity, metagenomics and/or transcriptomics studies of unicellular eukaryotes (protists). This sample has replicate sample(s): TARA_N010000336, TARA_N010000337, TARA_N010000339.

characteristic: description

text: Polar Biome

characteristic: environment (biome)

characteristic: environment (feature)

text: depth interval within the epipelagic zone (ENVO:00000209) including the photic zone (ENVO:00000209) and part of the aphotic zone (ENVO:00000210), including a strong peak in chlorophyll fluorescence, within a marine epipelagic wind mixed layer (ENVO:01000061)

characteristic: environment (feature) further details

text: particulate matter (ENVO:01000060), including planktonic material (ENVO:01000063)

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

characteristic: event label

characteristic: further details

characteristic: last update date

characteristic: latitude start

text: [AO] Arctic Ocean (MRGID:1906)

text: marine metagenome

characteristic: organism

text: Tara Oceans Expedition (2009-2013)

text: In the size fraction N180-2000 micrometres, the sample material was obtained from a net-cod-end collector lined with a nylon mesh of 180-micrometres and passed through a sieve with a metal mesh of 2000-micrometres. Filtered sea water (<0.1-micrometre) was added to the filtrate up to a total volume of 3 Litres. A volume of 500 mL from the diluted filtrate was filtered in parallel on two 10-micrometres-pore-size, 47-mm-diameter Millipore polycarbonate membrane filters (ref:TCTP04700), packaged in a sterile cryogenic tube (5 mL) (2 filters in 1 tube), labelled with a barcode identification sticker and stored on board in liquid nitrogen. Note 1: Latex or nitril gloves were used for this protocol. Note 2: All containers, filter holders and tubing were washed with 0.1% bleach, rinsed twice with fresh water and rinsed again twice with the pre-filtered sample material. All tweezers are kept clean with ethanol. Note 3: The entire process did not exceed 15 minutes. A total of 2 replicates were prepared and sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

text: SV Tara

text: marine metagenome

text: TARA_20130711T1225Z_175_EVENT_NET_MIX_(0-100m)_SEQ-(500mL-or-15min)_N180-2000_N010000338

characteristic: title


characteristic: Chlorophyll Sensor

text: Tara Oceans Consortium, Coordinators & Tara Oceans Expedition, Participants. Registry of all samples from the Tara Oceans Expedition (2009-2013). PANGAEA. doi:10.1594/PANGAEA.842197 (2015) (https://dx.doi.org/10.1594/PANGAEA.842197)

characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: GSC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

text: This sample (TARA_B110000208) was collected at station TARA_178 during campaign TARA_20130629Z of the Tara Oceans expedition (2009-2013), using a high volume peristaltic pump [HVP-PUMP]. The sampling event (TARA_20130715T0211Z_178_EVENT_PUMP) occurred at position latitudeN=77.1604 and longitudeE=73.2057, on date/time=2013-07-15T02:11:00, at a depth of 3-7 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated (0.22-3 micrometres) and prepared on board using protocol [SEQ-(100L)_W0.22-3] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes. This sample has replicate sample(s): TARA_B110000209.

text: Polar Biome

text: discrete depth within the epipelagic zone (ENVO:00000209) at the top of the photic zone (ENVO:00000209)

text: water

characteristic: longitude start

text: [AO] Arctic Ocean (MRGID:1906)

characteristic: marine region

characteristic: nitrate sensor

text: marine metagenome

characteristic: oxygen sensor

text: Tara Oceans Expedition (2009-2013)

characteristic: project name

text: In the size fraction W0.22-3 micrometres, the sample material was pre-filtered on 3-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane (ref:TSTP14250), and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 100 Litres was filtered and the 0.22-micrometre-pore-size filter was packaged in a sterile Falcon tube (50 mL), treated with the addition of 10 mL of RNA-Later, labelled with a barcode identification sticker and stored on board in a freezer at -20 degree Celsius. Note 1: Latex or nitril gloves were used for this protocol. Note 2: All containers, filter holders and tubing were washed with 0.1% bleach, rinsed twice with fresh water and rinsed again twice with the pre-filtered sample material. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump (Masterflex, EW-77410-10) was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. A total of 2 replicates were prepared and sent for later analysis to the Institute of Marine Science (CSIC), Barcelona, Spain.

characteristic: protocol description

characteristic: protocol label

text: high volume peristaltic pump [HVP-PUMP]

characteristic: sample collection device

characteristic: sample material label

characteristic: sample status

characteristic: sampling campaign

text: SV Tara

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: TARA_20130715T0211Z_178_EVENT_PUMP_SRF_(5m)_SEQ-(100L)_W0.22-3_B110000208


characteristic: Chlorophyll Sensor

characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: GSC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (TARA_N010000856) was collected at station TARA_178 during campaign TARA_20130629Z of the Tara Oceans expedition (2009-2013), using a high volume peristaltic pump [HVP-PUMP]. The sampling event (TARA_20130715T0211Z_178_EVENT_PUMP) occurred at position latitudeN=77.1604 and longitudeE=73.2057, on date/time=2013-07-15T02:11:00, at a depth of 3-7 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated (>0.8 micrometres) and prepared on board using protocol [SEQ-(100L-or-15min)_W>0.8] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity, metagenomics and/or transcriptomics studies of unicellular eukaryotes (protists). This sample has replicate sample(s): TARA_N010000857, TARA_N010000858, TARA_A100001578, TARA_N010000859, TARA_N010000860, TARA_N010000861. The sample material was split into several aliquot samples including this one and sample(s): TARA_N010000857.

characteristic: description

text: Polar Biome

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (feature) further details

text: water (ENVO:00002006), including particulate matter (ENVO:01000060) such as planktonic material (ENVO:01000063)

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

characteristic: event label

characteristic: further details

characteristic: last update date

characteristic: latitude start

text: [AO] Arctic Ocean (MRGID:1906)

text: marine metagenome

characteristic: organism

text: Tara Oceans Expedition (2009-2013)

text: In the size fraction W>0.8 micrometres, the sample material was concentrated on 0.8-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane filter (ref:ATTP14250) on top of a Dacron pad mesh. A volume of 100 Litres was filtered in parallel on two 0.8-micrometre-pore-size filters. Each filter was then packaged in a sterile cryogenic tube (5 mL) (2 tubes, each with 1 filter), labelled with a barcode identification sticker and stored on board in liquid nitrogen. Note 1: Latex or nitril gloves were used for this protocol. Note 2: All containers, filter holders and tubing were washed with 0.1% bleach, rinsed twice with fresh water and rinsed again twice with the pre-filtered sample material. All tweezers are kept clean with ethanol. Note 3: The entire process did not exceed 15 minutes. Note 4: The total volume filtered on the two filters is 100 Litres. However, the volume filtered on each filter is unknown, but approximately 50 Litres. The intention of this protocol is to combine the two filters during molecular entities (nucleotides) extraction and sequencing analysis. A total of 2 (2x 2 tubes) replicates were prepared and sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

text: high volume peristaltic pump [HVP-PUMP]

text: This version can be used to provide data discovery services

text: SV Tara

text: marine metagenome

text: TARA_20130715T0211Z_178_EVENT_PUMP_SRF_(5m)_SEQ-(100L-or-15min)_W>0.8_N010000856

characteristic: title


characteristic: Chlorophyll Sensor

characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: GSC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

text: This sample (TARA_N010000869) was collected at station TARA_178 during campaign TARA_20130629Z of the Tara Oceans expedition (2009-2013), using a high volume peristaltic pump [HVP-PUMP]. The sampling event (TARA_20130715T0211Z_178_EVENT_PUMP) occurred at position latitudeN=77.1604 and longitudeE=73.2057, on date/time=2013-07-15T02:11:00, at a depth of 3-7 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated (3-20 micrometres) and prepared on board using protocol [SEQ-(10-100L-or-15min)_W3-20] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity, metagenomics and/or transcriptomics studies of unicellular eukaryotes (protists) and attached prokaryotes. This sample has replicate sample(s): TARA_N010000870, TARA_N010000871, TARA_N010000872, TARA_N010000868.

text: Polar Biome

text: water

characteristic: longitude start

text: [AO] Arctic Ocean (MRGID:1906)

characteristic: marine region

characteristic: nitrate sensor

text: marine metagenome

characteristic: oxygen sensor

text: Tara Oceans Expedition (2009-2013)

characteristic: project name

text: In the size fraction W3-20 micrometres, the sample material was pre-filtered on 20-micrometres, using a nylon mesh, and concentrated on 3-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane filter (ref:TSTP14250). A volume of 10-100 Litres was filtered and the 3-micrometre-pore-size filter was packaged in a sterile cryogenic tube (5 mL), labelled with a barcode identification sticker and stored on board in liquid nitrogen. Note 1: Latex or nitril gloves were used for this protocol. Note 2: All containers, filter holders and tubing were washed with 0.1% bleach, rinsed twice with fresh water and rinsed again twice with the pre-filtered sample material. All tweezers are kept clean with ethanol. Note 3: The entire process did not exceed 15 minutes. Note 4: The volume filtered is indicated for each replicate in the Sample registry (http://doi.pangaea.de/10.1594/PANGAEA.842197). A total of 2-6 replicates were prepared and sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

characteristic: protocol description

characteristic: protocol label

text: high volume peristaltic pump [HVP-PUMP]

characteristic: sample collection device

characteristic: sample material label

characteristic: sample status

characteristic: sampling campaign

text: SV Tara

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: TARA_20130715T0211Z_178_EVENT_PUMP_SRF_(5m)_SEQ-(10-100L-or-15min)_W3-20_N010000869


characteristic: Chlorophyll Sensor

characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: GSC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (TARA_B110000204) was collected at station TARA_178 during campaign TARA_20130629Z of the Tara Oceans expedition (2009-2013), using a high volume peristaltic pump [HVP-PUMP]. The sampling event (TARA_20130715T0211Z_178_EVENT_PUMP) occurred at position latitudeN=77.1604 and longitudeE=73.2057, on date/time=2013-07-15T02:11:00, at a depth of 3-7 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated (0.22-3 micrometres) and prepared on board using protocol [SEQ-(10L-or-15min)_W0.22-3] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity, metagenomics and/or transcriptomics studies of prokaryotes. This sample has replicate sample(s): TARA_B110000205.

characteristic: description

text: Polar Biome

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (feature) further details

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

characteristic: event label

characteristic: further details

characteristic: last update date

characteristic: latitude start

text: [AO] Arctic Ocean (MRGID:1906)

text: marine metagenome

characteristic: organism

text: Tara Oceans Expedition (2009-2013)

text: In the size fraction W0.22-3 micrometres, the sample material was pre-filtered on 3-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane (ref:TSTP14250), and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 10 Litres was filtered and the 0.22-micrometre-pore-size filter was packaged in a sterile Falcon tube (50 mL), treated with the addition of 10 mL of RNA-Later, labelled with a barcode identification sticker and stored on board in a freezer at -20 degree Celsius. Note 1: Latex or nitril gloves were used for this protocol. Note 2: All containers, filter holders and tubing were washed with 0.1% bleach, rinsed twice with fresh water and rinsed again twice with the pre-filtered sample material. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump (Masterflex, EW-77410-10) was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The entire process did not exceed 15 minutes. A total of 2 replicates were prepared and sent for later analysis to the Institute of Marine Science (CSIC), Barcelona, Spain.

text: high volume peristaltic pump [HVP-PUMP]

text: SV Tara

text: marine metagenome

text: TARA_20130715T0211Z_178_EVENT_PUMP_SRF_(5m)_SEQ-(10L-or-15min)_W0.22-3_B110000204

characteristic: title


characteristic: Chlorophyll Sensor

characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: GSC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

text: This sample (TARA_R110002124) was collected at station TARA_178 during campaign TARA_20130629Z of the Tara Oceans expedition (2009-2013), using a high volume peristaltic pump [HVP-PUMP]. The sampling event (TARA_20130715T0211Z_178_EVENT_PUMP) occurred at position latitudeN=77.1604 and longitudeE=73.2057, on date/time=2013-07-15T02:11:00, at a depth of 3-7 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated (<0.22 micrometres) and prepared on board using protocol [SEQ-(FeCl3)_W<0.22] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of viruses. This sample has no known replicate.

text: Polar Biome

text: water

characteristic: longitude start

text: [AO] Arctic Ocean (MRGID:1906)

characteristic: marine region

characteristic: nitrate sensor

text: marine metagenome

characteristic: oxygen sensor

text: Tara Oceans Expedition (2009-2013)

characteristic: project name

text: In the size fraction W<0.22 micrometres, the sample material was pre-filtered using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane (ref:GPWP14250) with 0.22-micrometres-pore-size. A volume of 20 Litres was treated with FeCl3, incubated at room temperature for 1-24 hours to allow viruses to precipitate, filtered onto a 1-micrometre-pore-size, 142-mm-diameter GE polycarbonate (PC) membrane filter (ref:K10CP14220) placed on top of a 0.8-micrometre-pore-size, 142-mm-diameter Pall Gelman support filter (ref:60114) using a peristaltic pump with pressure <15 psi. The 1-micrometre-pore-size filter was then packaged in a sterile Falcon tube (50 mL) (up to 3 filters in 1 tube), labelled with a barcode identification sticker and stored on board in a refrigerator at +4 degree Celsius. Note 1: Latex or nitril gloves were used for this protocol. Note 2: All containers, filter holders and tubing were washed with 0.1% bleach, rinsed twice with fresh water and rinsed again twice with the pre-filtered sample material. All tweezers are kept clean with ethanol. Note 3: The three replicates were incubated together. For 20 Litres of filtrate, 2 millilitres of FeCl3 stock solution (4.83 grams of FeCl3-6H2O + 100 millilitres of MilliQ water, stored at 4°C) was added (final concentration of 1 mg Fe per Litre of filtrate), and shaken vigorously for 1 minute. Note 4: Further details about the method are published here doi:10.1111/j.1758-2229.2010.00208.x. In 2009-2012, a total of 3 replicates were prepared and sent for later analysis to the University of Arizona (UA), Tucson, USA. In 2013, a total of 2 replicates were prepared, one sent to for later analysis to the University of Arizona (UA), Tucson, USA, and the other sent for later analysis to the Observatoire Océanologique de Banyuls sur Mer (OOB), France.

characteristic: protocol description

characteristic: protocol label

text: high volume peristaltic pump [HVP-PUMP]

characteristic: sample collection device

characteristic: sample material label

characteristic: sample status

characteristic: sampling campaign

text: SV Tara

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: TARA_20130715T0211Z_178_EVENT_PUMP_SRF_(5m)_SEQ-(FeCl3)_W<0.22_R110002124


characteristic: Chlorophyll Sensor

text: Tara Oceans Consortium, Coordinators & Tara Oceans Expedition, Participants. Registry of all samples from the Tara Oceans Expedition (2009-2013). PANGAEA. doi:10.1594/PANGAEA.842197 (2015) (https://dx.doi.org/10.1594/PANGAEA.842197)

characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: GSC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (TARA_N010000854) was collected at station TARA_178 during campaign TARA_20130629Z of the Tara Oceans expedition (2009-2013), using a plankton net [NET-DOUBLE-20]+[SIEVE-180]. The sampling event (TARA_20130715T0406Z_178_EVENT_NET) occurred at position latitudeN=77.2302 and longitudeE=73.1903, on date/time=2013-07-15T04:06:00, at a depth of 3-7 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated (20-180 micrometres) and prepared on board using protocol [SEQ-(500mL-or-15min)_N20-180] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity, metagenomics and/or transcriptomics studies of unicellular eukaryotes (protists). This sample has replicate sample(s): TARA_N010000852, TARA_N010000853, TARA_N010000855.

characteristic: description

text: Polar Biome

characteristic: environment (biome)

characteristic: environment (feature)

text: discrete depth within the epipelagic zone (ENVO:00000209) at the top of the photic zone (ENVO:00000209)

characteristic: environment (feature) further details

text: particulate matter (ENVO:01000060), including planktonic material (ENVO:01000063)

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

characteristic: event label

characteristic: further details

characteristic: last update date

characteristic: latitude start

text: [AO] Arctic Ocean (MRGID:1906)

text: marine metagenome

characteristic: organism

text: Tara Oceans Expedition (2009-2013)

text: In the size fraction N20-180 micrometres, the sample material was obtained from a net-cod-end collector lined with a nylon mesh of 20-micrometres and passed through a sieve with a metal mesh of 180-micrometres. Filtered sea water (<0.1-micrometre) was added to the filtrate up to a total volume of 3 Litres. A volume of 500 mL from the diluted filtrate was filtered in parallel on two 10-micrometres-pore-size, 47-mm-diameter Millipore polycarbonate membrane filters (ref:TCTP04700), packaged in a sterile cryogenic tube (5 mL) (2 filters in 1 tube), labelled with a barcode identification sticker and stored on board in liquid nitrogen. Note 1: Latex or nitril gloves were used for this protocol. Note 2: All containers, filter holders and tubing were washed with 0.1% bleach, rinsed twice with fresh water and rinsed again twice with the pre-filtered sample material. All tweezers are kept clean with ethanol. Note 3: The entire process did not exceed 15 minutes. A total of 2 replicates were prepared and sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

text: SV Tara

text: marine metagenome

text: TARA_20130715T0406Z_178_EVENT_NET_SRF_(5m)_SEQ-(500mL-or-15min)_N20-180_N010000854

characteristic: title


characteristic: Chlorophyll Sensor

characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: GSC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

text: This sample (TARA_N010000846) was collected at station TARA_178 during campaign TARA_20130629Z of the Tara Oceans expedition (2009-2013), using a plankton net [NET-BONGO-180]+[SIEVE-2000]. The sampling event (TARA_20130715T0608Z_178_EVENT_NET) occurred at position latitudeN=77.3047 and longitudeE=73.2462, on date/time=2013-07-15T06:08:00, at a depth of 3-7 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated (180-2000 micrometres) and prepared on board using protocol [SEQ-(500mL-or-15min)_N180-2000] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity, metagenomics and/or transcriptomics studies of unicellular eukaryotes (protists). This sample has replicate sample(s): TARA_N010000844, TARA_N010000845, TARA_N010000847.

text: Polar Biome

text: water

characteristic: longitude start

text: [AO] Arctic Ocean (MRGID:1906)

characteristic: marine region

characteristic: nitrate sensor

text: marine metagenome

characteristic: oxygen sensor

text: Tara Oceans Expedition (2009-2013)

characteristic: project name

text: In the size fraction N180-2000 micrometres, the sample material was obtained from a net-cod-end collector lined with a nylon mesh of 180-micrometres and passed through a sieve with a metal mesh of 2000-micrometres. Filtered sea water (<0.1-micrometre) was added to the filtrate up to a total volume of 3 Litres. A volume of 500 mL from the diluted filtrate was filtered in parallel on two 10-micrometres-pore-size, 47-mm-diameter Millipore polycarbonate membrane filters (ref:TCTP04700), packaged in a sterile cryogenic tube (5 mL) (2 filters in 1 tube), labelled with a barcode identification sticker and stored on board in liquid nitrogen. Note 1: Latex or nitril gloves were used for this protocol. Note 2: All containers, filter holders and tubing were washed with 0.1% bleach, rinsed twice with fresh water and rinsed again twice with the pre-filtered sample material. All tweezers are kept clean with ethanol. Note 3: The entire process did not exceed 15 minutes. A total of 2 replicates were prepared and sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

characteristic: protocol description

characteristic: protocol label

characteristic: sample collection device

characteristic: sample material label

text: This version can be used to provide data discovery services

characteristic: sample status

characteristic: sampling campaign

text: SV Tara

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: TARA_20130715T0608Z_178_EVENT_NET_SRF_(5m)_SEQ-(500mL-or-15min)_N180-2000_N010000846


characteristic: Chlorophyll Sensor

characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: GSC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (TARA_B110000503) was collected at station TARA_180 during campaign TARA_20130629Z of the Tara Oceans expedition (2009-2013), using a high volume peristaltic pump [HVP-PUMP]. The sampling event (TARA_20130718T0204Z_180_EVENT_PUMP) occurred at position latitudeN=74.8023 and longitudeE=76.1478, on date/time=2013-07-18T02:04:00, at a depth of 3-7 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated (0.22-3 micrometres) and prepared on board using protocol [SEQ-(100L)_W0.22-3] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes. This sample has replicate sample(s): TARA_B110000504.

characteristic: description

text: Polar Biome

characteristic: environment (biome)

characteristic: environment (feature)

text: discrete depth within the epipelagic zone (ENVO:00000209) at the top of the photic zone (ENVO:00000209), <50 m from a strong peak in chlorophyll fluorescence, within <50 m from the sea floor (ENVO:00000482)

characteristic: environment (feature) further details

text: water (ENVO:00002006), including particulate matter (ENVO:01000060) such as planktonic material (ENVO:01000063)

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

characteristic: event label

characteristic: further details

characteristic: last update date

characteristic: latitude start

text: [AO] Arctic Ocean (MRGID:1906)

text: marine metagenome

characteristic: organism

text: Tara Oceans Expedition (2009-2013)

text: In the size fraction W0.22-3 micrometres, the sample material was pre-filtered on 3-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane (ref:TSTP14250), and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 100 Litres was filtered and the 0.22-micrometre-pore-size filter was packaged in a sterile Falcon tube (50 mL), treated with the addition of 10 mL of RNA-Later, labelled with a barcode identification sticker and stored on board in a freezer at -20 degree Celsius. Note 1: Latex or nitril gloves were used for this protocol. Note 2: All containers, filter holders and tubing were washed with 0.1% bleach, rinsed twice with fresh water and rinsed again twice with the pre-filtered sample material. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump (Masterflex, EW-77410-10) was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. A total of 2 replicates were prepared and sent for later analysis to the Institute of Marine Science (CSIC), Barcelona, Spain.

text: high volume peristaltic pump [HVP-PUMP]

text: SV Tara

text: marine metagenome

text: TARA_20130718T0204Z_180_EVENT_PUMP_SRF_(5m)_SEQ-(100L)_W0.22-3_B110000503

characteristic: title


characteristic: Chlorophyll Sensor

characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: GSC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

text: This sample (TARA_N010000873) was collected at station TARA_180 during campaign TARA_20130629Z of the Tara Oceans expedition (2009-2013), using a high volume peristaltic pump [HVP-PUMP]. The sampling event (TARA_20130718T0204Z_180_EVENT_PUMP) occurred at position latitudeN=74.8023 and longitudeE=76.1478, on date/time=2013-07-18T02:04:00, at a depth of 3-7 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated (>0.8 micrometres) and prepared on board using protocol [SEQ-(100L-or-15min)_W>0.8] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity, metagenomics and/or transcriptomics studies of unicellular eukaryotes (protists). This sample has replicate sample(s): TARA_N010000874, TARA_N010000875, TARA_N010000883. The sample material was split into several aliquot samples including this one and sample(s): TARA_N010000874.

text: Polar Biome

text: water

characteristic: longitude start

text: [AO] Arctic Ocean (MRGID:1906)

characteristic: marine region

characteristic: nitrate sensor

text: marine metagenome

characteristic: oxygen sensor

text: Tara Oceans Expedition (2009-2013)

characteristic: project name

text: In the size fraction W>0.8 micrometres, the sample material was concentrated on 0.8-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane filter (ref:ATTP14250) on top of a Dacron pad mesh. A volume of 100 Litres was filtered in parallel on two 0.8-micrometre-pore-size filters. Each filter was then packaged in a sterile cryogenic tube (5 mL) (2 tubes, each with 1 filter), labelled with a barcode identification sticker and stored on board in liquid nitrogen. Note 1: Latex or nitril gloves were used for this protocol. Note 2: All containers, filter holders and tubing were washed with 0.1% bleach, rinsed twice with fresh water and rinsed again twice with the pre-filtered sample material. All tweezers are kept clean with ethanol. Note 3: The entire process did not exceed 15 minutes. Note 4: The total volume filtered on the two filters is 100 Litres. However, the volume filtered on each filter is unknown, but approximately 50 Litres. The intention of this protocol is to combine the two filters during molecular entities (nucleotides) extraction and sequencing analysis. A total of 2 (2x 2 tubes) replicates were prepared and sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

characteristic: protocol description

characteristic: protocol label

text: high volume peristaltic pump [HVP-PUMP]

characteristic: sample collection device

characteristic: sample material label

characteristic: sample status

characteristic: sampling campaign

text: SV Tara

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: TARA_20130718T0204Z_180_EVENT_PUMP_SRF_(5m)_SEQ-(100L-or-15min)_W>0.8_N010000873


characteristic: Chlorophyll Sensor

text: Tara Oceans Consortium, Coordinators & Tara Oceans Expedition, Participants. Registry of all samples from the Tara Oceans Expedition (2009-2013). PANGAEA. doi:10.1594/PANGAEA.842197 (2015) (https://dx.doi.org/10.1594/PANGAEA.842197)

characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: GSC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (TARA_N010000885) was collected at station TARA_180 during campaign TARA_20130629Z of the Tara Oceans expedition (2009-2013), using a high volume peristaltic pump [HVP-PUMP]. The sampling event (TARA_20130718T0204Z_180_EVENT_PUMP) occurred at position latitudeN=74.8023 and longitudeE=76.1478, on date/time=2013-07-18T02:04:00, at a depth of 3-7 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated (3-20 micrometres) and prepared on board using protocol [SEQ-(10-100L-or-15min)_W3-20] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity, metagenomics and/or transcriptomics studies of unicellular eukaryotes (protists) and attached prokaryotes. This sample has replicate sample(s): TARA_N010000886, TARA_N010000887, TARA_N010000888, TARA_N010000884.

characteristic: description

text: Polar Biome

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (feature) further details

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

characteristic: event label

characteristic: further details

characteristic: last update date

characteristic: latitude start

text: [AO] Arctic Ocean (MRGID:1906)

text: marine metagenome

characteristic: organism

text: Tara Oceans Expedition (2009-2013)

text: In the size fraction W3-20 micrometres, the sample material was pre-filtered on 20-micrometres, using a nylon mesh, and concentrated on 3-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane filter (ref:TSTP14250). A volume of 10-100 Litres was filtered and the 3-micrometre-pore-size filter was packaged in a sterile cryogenic tube (5 mL), labelled with a barcode identification sticker and stored on board in liquid nitrogen. Note 1: Latex or nitril gloves were used for this protocol. Note 2: All containers, filter holders and tubing were washed with 0.1% bleach, rinsed twice with fresh water and rinsed again twice with the pre-filtered sample material. All tweezers are kept clean with ethanol. Note 3: The entire process did not exceed 15 minutes. Note 4: The volume filtered is indicated for each replicate in the Sample registry (http://doi.pangaea.de/10.1594/PANGAEA.842197). A total of 2-6 replicates were prepared and sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

text: high volume peristaltic pump [HVP-PUMP]

text: SV Tara

text: marine metagenome

text: TARA_20130718T0204Z_180_EVENT_PUMP_SRF_(5m)_SEQ-(10-100L-or-15min)_W3-20_N010000885

characteristic: title


characteristic: Chlorophyll Sensor

characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: GSC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

text: This sample (TARA_B110000501) was collected at station TARA_180 during campaign TARA_20130629Z of the Tara Oceans expedition (2009-2013), using a high volume peristaltic pump [HVP-PUMP]. The sampling event (TARA_20130718T0204Z_180_EVENT_PUMP) occurred at position latitudeN=74.8023 and longitudeE=76.1478, on date/time=2013-07-18T02:04:00, at a depth of 3-7 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated (0.22-3 micrometres) and prepared on board using protocol [SEQ-(10L-or-15min)_W0.22-3] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity, metagenomics and/or transcriptomics studies of prokaryotes. This sample has replicate sample(s): TARA_B110000502.

text: Polar Biome

text: water

characteristic: longitude start

text: [AO] Arctic Ocean (MRGID:1906)

characteristic: marine region

characteristic: nitrate sensor

text: marine metagenome

characteristic: oxygen sensor

text: Tara Oceans Expedition (2009-2013)

characteristic: project name

text: In the size fraction W0.22-3 micrometres, the sample material was pre-filtered on 3-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane (ref:TSTP14250), and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 10 Litres was filtered and the 0.22-micrometre-pore-size filter was packaged in a sterile Falcon tube (50 mL), treated with the addition of 10 mL of RNA-Later, labelled with a barcode identification sticker and stored on board in a freezer at -20 degree Celsius. Note 1: Latex or nitril gloves were used for this protocol. Note 2: All containers, filter holders and tubing were washed with 0.1% bleach, rinsed twice with fresh water and rinsed again twice with the pre-filtered sample material. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump (Masterflex, EW-77410-10) was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The entire process did not exceed 15 minutes. A total of 2 replicates were prepared and sent for later analysis to the Institute of Marine Science (CSIC), Barcelona, Spain.

characteristic: protocol description

characteristic: protocol label

text: high volume peristaltic pump [HVP-PUMP]

characteristic: sample collection device

characteristic: sample material label

characteristic: sample status

characteristic: sampling campaign

text: SV Tara

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: TARA_20130718T0204Z_180_EVENT_PUMP_SRF_(5m)_SEQ-(10L-or-15min)_W0.22-3_B110000501


characteristic: Chlorophyll Sensor

characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: GSC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (TARA_R110000868) was collected at station TARA_180 during campaign TARA_20130629Z of the Tara Oceans expedition (2009-2013), using a high volume peristaltic pump [HVP-PUMP]. The sampling event (TARA_20130718T0204Z_180_EVENT_PUMP) occurred at position latitudeN=74.8023 and longitudeE=76.1478, on date/time=2013-07-18T02:04:00, at a depth of 3-7 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated (<0.22 micrometres) and prepared on board using protocol [SEQ-(FeCl3)_W<0.22] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of viruses. This sample has no known replicate.

characteristic: description

text: Polar Biome

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (feature) further details

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

characteristic: event label

characteristic: further details

characteristic: last update date

characteristic: latitude start

text: [AO] Arctic Ocean (MRGID:1906)

text: marine metagenome

characteristic: organism

text: Tara Oceans Expedition (2009-2013)

text: In the size fraction W<0.22 micrometres, the sample material was pre-filtered using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane (ref:GPWP14250) with 0.22-micrometres-pore-size. A volume of 20 Litres was treated with FeCl3, incubated at room temperature for 1-24 hours to allow viruses to precipitate, filtered onto a 1-micrometre-pore-size, 142-mm-diameter GE polycarbonate (PC) membrane filter (ref:K10CP14220) placed on top of a 0.8-micrometre-pore-size, 142-mm-diameter Pall Gelman support filter (ref:60114) using a peristaltic pump with pressure <15 psi. The 1-micrometre-pore-size filter was then packaged in a sterile Falcon tube (50 mL) (up to 3 filters in 1 tube), labelled with a barcode identification sticker and stored on board in a refrigerator at +4 degree Celsius. Note 1: Latex or nitril gloves were used for this protocol. Note 2: All containers, filter holders and tubing were washed with 0.1% bleach, rinsed twice with fresh water and rinsed again twice with the pre-filtered sample material. All tweezers are kept clean with ethanol. Note 3: The three replicates were incubated together. For 20 Litres of filtrate, 2 millilitres of FeCl3 stock solution (4.83 grams of FeCl3-6H2O + 100 millilitres of MilliQ water, stored at 4°C) was added (final concentration of 1 mg Fe per Litre of filtrate), and shaken vigorously for 1 minute. Note 4: Further details about the method are published here doi:10.1111/j.1758-2229.2010.00208.x. In 2009-2012, a total of 3 replicates were prepared and sent for later analysis to the University of Arizona (UA), Tucson, USA. In 2013, a total of 2 replicates were prepared, one sent to for later analysis to the University of Arizona (UA), Tucson, USA, and the other sent for later analysis to the Observatoire Océanologique de Banyuls sur Mer (OOB), France.

text: high volume peristaltic pump [HVP-PUMP]

text: This version can be used to provide data discovery services

text: SV Tara

text: marine metagenome

text: TARA_20130718T0204Z_180_EVENT_PUMP_SRF_(5m)_SEQ-(FeCl3)_W<0.22_R110000868

characteristic: title


characteristic: Chlorophyll Sensor

characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: GSC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

text: This sample (TARA_N010000895) was collected at station TARA_180 during campaign TARA_20130629Z of the Tara Oceans expedition (2009-2013), using a plankton net [NET-BONGO-180]+[SIEVE-2000]. The sampling event (TARA_20130718T0305Z_180_EVENT_NET) occurred at position latitudeN=74.8063 and longitudeE=76.1759, on date/time=2013-07-18T03:05:00, at a depth of 3-7 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated (180-2000 micrometres) and prepared on board using protocol [SEQ-(500mL-or-15min)_N180-2000] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity, metagenomics and/or transcriptomics studies of unicellular eukaryotes (protists). This sample has replicate sample(s): TARA_N010000893, TARA_N010000894, TARA_N010000896.

text: Polar Biome

text: discrete depth within the epipelagic zone (ENVO:00000209) at the top of the photic zone (ENVO:00000209), <50 m from a strong peak in chlorophyll fluorescence, within <50 m from the sea floor (ENVO:00000482)

text: particulate matter (ENVO:01000060), including planktonic material (ENVO:01000063)

text: water

characteristic: longitude start

text: [AO] Arctic Ocean (MRGID:1906)

characteristic: marine region

characteristic: nitrate sensor

text: marine metagenome

characteristic: oxygen sensor

text: Tara Oceans Expedition (2009-2013)

characteristic: project name

text: In the size fraction N180-2000 micrometres, the sample material was obtained from a net-cod-end collector lined with a nylon mesh of 180-micrometres and passed through a sieve with a metal mesh of 2000-micrometres. Filtered sea water (<0.1-micrometre) was added to the filtrate up to a total volume of 3 Litres. A volume of 500 mL from the diluted filtrate was filtered in parallel on two 10-micrometres-pore-size, 47-mm-diameter Millipore polycarbonate membrane filters (ref:TCTP04700), packaged in a sterile cryogenic tube (5 mL) (2 filters in 1 tube), labelled with a barcode identification sticker and stored on board in liquid nitrogen. Note 1: Latex or nitril gloves were used for this protocol. Note 2: All containers, filter holders and tubing were washed with 0.1% bleach, rinsed twice with fresh water and rinsed again twice with the pre-filtered sample material. All tweezers are kept clean with ethanol. Note 3: The entire process did not exceed 15 minutes. A total of 2 replicates were prepared and sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

characteristic: protocol description

characteristic: protocol label

characteristic: sample collection device

characteristic: sample material label

characteristic: sample status

characteristic: sampling campaign

text: SV Tara

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: TARA_20130718T0305Z_180_EVENT_NET_SRF_(5m)_SEQ-(500mL-or-15min)_N180-2000_N010000895


characteristic: Chlorophyll Sensor

characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: GSC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (TARA_N010000889) was collected at station TARA_180 during campaign TARA_20130629Z of the Tara Oceans expedition (2009-2013), using a plankton net [NET-DOUBLE-20]+[SIEVE-180]. The sampling event (TARA_20130718T0451Z_180_EVENT_NET) occurred at position latitudeN=74.8039 and longitudeE=76.0932, on date/time=2013-07-18T04:51:00, at a depth of 3-7 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated (20-180 micrometres) and prepared on board using protocol [SEQ-(500mL-or-15min)_N20-180] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity, metagenomics and/or transcriptomics studies of unicellular eukaryotes (protists). This sample has replicate sample(s): TARA_N010000890, TARA_N010000891, TARA_N010000892.

characteristic: description

text: Polar Biome

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (feature) further details

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

characteristic: event label

characteristic: further details

characteristic: last update date

characteristic: latitude start

text: [AO] Arctic Ocean (MRGID:1906)

text: marine metagenome

characteristic: organism

text: Tara Oceans Expedition (2009-2013)

text: In the size fraction N20-180 micrometres, the sample material was obtained from a net-cod-end collector lined with a nylon mesh of 20-micrometres and passed through a sieve with a metal mesh of 180-micrometres. Filtered sea water (<0.1-micrometre) was added to the filtrate up to a total volume of 3 Litres. A volume of 500 mL from the diluted filtrate was filtered in parallel on two 10-micrometres-pore-size, 47-mm-diameter Millipore polycarbonate membrane filters (ref:TCTP04700), packaged in a sterile cryogenic tube (5 mL) (2 filters in 1 tube), labelled with a barcode identification sticker and stored on board in liquid nitrogen. Note 1: Latex or nitril gloves were used for this protocol. Note 2: All containers, filter holders and tubing were washed with 0.1% bleach, rinsed twice with fresh water and rinsed again twice with the pre-filtered sample material. All tweezers are kept clean with ethanol. Note 3: The entire process did not exceed 15 minutes. A total of 2 replicates were prepared and sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

text: SV Tara

text: marine metagenome

text: TARA_20130718T0451Z_180_EVENT_NET_SRF_(5m)_SEQ-(500mL-or-15min)_N20-180_N010000889

characteristic: title


characteristic: Chlorophyll Sensor

text: Tara Oceans Consortium, Coordinators & Tara Oceans Expedition, Participants. Registry of all samples from the Tara Oceans Expedition (2009-2013). PANGAEA. doi:10.1594/PANGAEA.842197 (2015) (https://dx.doi.org/10.1594/PANGAEA.842197)

characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: GSC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

text: This sample (TARA_B110000211) was collected at station TARA_180 during campaign TARA_20130629Z of the Tara Oceans expedition (2009-2013), using a high volume peristaltic pump [HVP-PUMP]. The sampling event (TARA_20130718T0827Z_180_EVENT_PUMP) occurred at position latitudeN=74.8283 and longitudeE=76.2096, on date/time=2013-07-18T08:27:00, at a depth of 28-32 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [DCM] deep chlorophyll maximum layer (ENVO:01000326). The sample material was size-fractionated (0.22-3 micrometres) and prepared on board using protocol [SEQ-(100L)_W0.22-3] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes. This sample has replicate sample(s): TARA_B110000210.

text: Polar Biome

text: discrete depth within the epipelagic zone (ENVO:00000209) at the interface of the photic zone (ENVO:00000209) and the aphotic zone (ENVO:00000210), <10 m from a strong peak in chlorophyll fluorescence, within <50 m from the sea floor (ENVO:00000482)

text: water (ENVO:00002006), including particulate matter (ENVO:01000060) such as planktonic material (ENVO:01000063)

text: water

characteristic: longitude start

text: [AO] Arctic Ocean (MRGID:1906)

characteristic: marine region

characteristic: nitrate sensor

text: marine metagenome

characteristic: oxygen sensor

text: Tara Oceans Expedition (2009-2013)

characteristic: project name

text: In the size fraction W0.22-3 micrometres, the sample material was pre-filtered on 3-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane (ref:TSTP14250), and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 100 Litres was filtered and the 0.22-micrometre-pore-size filter was packaged in a sterile Falcon tube (50 mL), treated with the addition of 10 mL of RNA-Later, labelled with a barcode identification sticker and stored on board in a freezer at -20 degree Celsius. Note 1: Latex or nitril gloves were used for this protocol. Note 2: All containers, filter holders and tubing were washed with 0.1% bleach, rinsed twice with fresh water and rinsed again twice with the pre-filtered sample material. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump (Masterflex, EW-77410-10) was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. A total of 2 replicates were prepared and sent for later analysis to the Institute of Marine Science (CSIC), Barcelona, Spain.

characteristic: protocol description

characteristic: protocol label

text: high volume peristaltic pump [HVP-PUMP]

characteristic: sample collection device

characteristic: sample material label

characteristic: sample status

characteristic: sampling campaign

text: SV Tara

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: TARA_20130718T0827Z_180_EVENT_PUMP_DCM_(30m)_SEQ-(100L)_W0.22-3_B110000211


characteristic: Chlorophyll Sensor

characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: GSC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (TARA_N010000862) was collected at station TARA_180 during campaign TARA_20130629Z of the Tara Oceans expedition (2009-2013), using a high volume peristaltic pump [HVP-PUMP]. The sampling event (TARA_20130718T0827Z_180_EVENT_PUMP) occurred at position latitudeN=74.8283 and longitudeE=76.2096, on date/time=2013-07-18T08:27:00, at a depth of 28-32 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [DCM] deep chlorophyll maximum layer (ENVO:01000326). The sample material was size-fractionated (>0.8 micrometres) and prepared on board using protocol [SEQ-(100L-or-15min)_W>0.8] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity, metagenomics and/or transcriptomics studies of unicellular eukaryotes (protists). This sample has replicate sample(s): TARA_N010000863, TARA_N010000864, TARA_N010000865, TARA_N010000866. The sample material was split into several aliquot samples including this one and sample(s): TARA_N010000863.

characteristic: description

text: Polar Biome

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (feature) further details

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

characteristic: event label

characteristic: further details

characteristic: last update date

characteristic: latitude start

text: [AO] Arctic Ocean (MRGID:1906)

text: marine metagenome

characteristic: organism

text: Tara Oceans Expedition (2009-2013)

text: In the size fraction W>0.8 micrometres, the sample material was concentrated on 0.8-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane filter (ref:ATTP14250) on top of a Dacron pad mesh. A volume of 100 Litres was filtered in parallel on two 0.8-micrometre-pore-size filters. Each filter was then packaged in a sterile cryogenic tube (5 mL) (2 tubes, each with 1 filter), labelled with a barcode identification sticker and stored on board in liquid nitrogen. Note 1: Latex or nitril gloves were used for this protocol. Note 2: All containers, filter holders and tubing were washed with 0.1% bleach, rinsed twice with fresh water and rinsed again twice with the pre-filtered sample material. All tweezers are kept clean with ethanol. Note 3: The entire process did not exceed 15 minutes. Note 4: The total volume filtered on the two filters is 100 Litres. However, the volume filtered on each filter is unknown, but approximately 50 Litres. The intention of this protocol is to combine the two filters during molecular entities (nucleotides) extraction and sequencing analysis. A total of 2 (2x 2 tubes) replicates were prepared and sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

text: high volume peristaltic pump [HVP-PUMP]

text: SV Tara

text: marine metagenome

text: TARA_20130718T0827Z_180_EVENT_PUMP_DCM_(30m)_SEQ-(100L-or-15min)_W>0.8_N010000862

characteristic: title


characteristic: Chlorophyll Sensor

characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: GSC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

text: This sample (TARA_N010000880) was collected at station TARA_180 during campaign TARA_20130629Z of the Tara Oceans expedition (2009-2013), using a high volume peristaltic pump [HVP-PUMP]. The sampling event (TARA_20130718T0827Z_180_EVENT_PUMP) occurred at position latitudeN=74.8283 and longitudeE=76.2096, on date/time=2013-07-18T08:27:00, at a depth of 28-32 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [DCM] deep chlorophyll maximum layer (ENVO:01000326). The sample material was size-fractionated (3-20 micrometres) and prepared on board using protocol [SEQ-(10-100L-or-15min)_W3-20] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity, metagenomics and/or transcriptomics studies of unicellular eukaryotes (protists) and attached prokaryotes. This sample has replicate sample(s): TARA_N010000881, TARA_N010000882, TARA_N010000879, TARA_N010000878.

text: Polar Biome

text: water

characteristic: longitude start

text: [AO] Arctic Ocean (MRGID:1906)

characteristic: marine region

characteristic: nitrate sensor

text: marine metagenome

characteristic: oxygen sensor

text: Tara Oceans Expedition (2009-2013)

characteristic: project name

text: In the size fraction W3-20 micrometres, the sample material was pre-filtered on 20-micrometres, using a nylon mesh, and concentrated on 3-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane filter (ref:TSTP14250). A volume of 10-100 Litres was filtered and the 3-micrometre-pore-size filter was packaged in a sterile cryogenic tube (5 mL), labelled with a barcode identification sticker and stored on board in liquid nitrogen. Note 1: Latex or nitril gloves were used for this protocol. Note 2: All containers, filter holders and tubing were washed with 0.1% bleach, rinsed twice with fresh water and rinsed again twice with the pre-filtered sample material. All tweezers are kept clean with ethanol. Note 3: The entire process did not exceed 15 minutes. Note 4: The volume filtered is indicated for each replicate in the Sample registry (http://doi.pangaea.de/10.1594/PANGAEA.842197). A total of 2-6 replicates were prepared and sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

characteristic: protocol description

characteristic: protocol label

text: high volume peristaltic pump [HVP-PUMP]

characteristic: sample collection device

characteristic: sample material label

text: This version can be used to provide data discovery services

characteristic: sample status

characteristic: sampling campaign

text: SV Tara

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: TARA_20130718T0827Z_180_EVENT_PUMP_DCM_(30m)_SEQ-(10-100L-or-15min)_W3-20_N010000880


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: not available

characteristic: collection date

text: not available

characteristic: country

text: Homo sapiens

characteristic: host

text: Yes

characteristic: host associated

text: Staphylococcus aureus

characteristic: organism

text: Staphylococcus aureus

characteristic: title


characteristic: Chlorophyll Sensor

characteristic: Citation

characteristic: Event Date/Time End

text: GSC

text: public

characteristic: Latitude End

characteristic: Longitude End

characteristic: Salinity Sensor

characteristic: depth

text: This sample (TARA_B110000206) was collected at station TARA_180 during campaign TARA_20130629Z of the Tara Oceans expedition (2009-2013), using a high volume peristaltic pump [HVP-PUMP]. The sampling event (TARA_20130718T0827Z_180_EVENT_PUMP) occurred at position latitudeN=74.8283 and longitudeE=76.2096, on date/time=2013-07-18T08:27:00, at a depth of 28-32 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [DCM] deep chlorophyll maximum layer (ENVO:01000326). The sample material was size-fractionated (0.22-3 micrometres) and prepared on board using protocol [SEQ-(10L-or-15min)_W0.22-3] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity, metagenomics and/or transcriptomics studies of prokaryotes. This sample has replicate sample(s): TARA_B110000207.

characteristic: description

text: Polar Biome

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (feature) further details

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

characteristic: event label

characteristic: further details

characteristic: last update date

characteristic: latitude start

characteristic: longitude start

text: [AO] Arctic Ocean (MRGID:1906)

characteristic: marine region

characteristic: nitrate sensor

text: marine metagenome

characteristic: oxygen sensor

text: Tara Oceans Expedition (2009-2013)

characteristic: project name

text: In the size fraction W0.22-3 micrometres, the sample material was pre-filtered on 3-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane (ref:TSTP14250), and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 10 Litres was filtered and the 0.22-micrometre-pore-size filter was packaged in a sterile Falcon tube (50 mL), treated with the addition of 10 mL of RNA-Later, labelled with a barcode identification sticker and stored on board in a freezer at -20 degree Celsius. Note 1: Latex or nitril gloves were used for this protocol. Note 2: All containers, filter holders and tubing were washed with 0.1% bleach, rinsed twice with fresh water and rinsed again twice with the pre-filtered sample material. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump (Masterflex, EW-77410-10) was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The entire process did not exceed 15 minutes. A total of 2 replicates were prepared and sent for later analysis to the Institute of Marine Science (CSIC), Barcelona, Spain.

characteristic: protocol description

characteristic: protocol label

text: high volume peristaltic pump [HVP-PUMP]

characteristic: sample collection device

characteristic: sample material label

characteristic: sample status

characteristic: sampling campaign

text: SV Tara

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: TARA_20130718T0827Z_180_EVENT_PUMP_DCM_(30m)_SEQ-(10L-or-15min)_W0.22-3_B110000206


characteristic: Chlorophyll Sensor

characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: GSC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (TARA_R110002126) was collected at station TARA_180 during campaign TARA_20130629Z of the Tara Oceans expedition (2009-2013), using a high volume peristaltic pump [HVP-PUMP]. The sampling event (TARA_20130718T0827Z_180_EVENT_PUMP) occurred at position latitudeN=74.8283 and longitudeE=76.2096, on date/time=2013-07-18T08:27:00, at a depth of 28-32 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [DCM] deep chlorophyll maximum layer (ENVO:01000326). The sample material was size-fractionated (<0.22 micrometres) and prepared on board using protocol [SEQ-(FeCl3)_W<0.22] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of viruses. This sample has no known replicate.

characteristic: description

text: Polar Biome

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (feature) further details

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

characteristic: event label

characteristic: further details

characteristic: last update date

characteristic: latitude start

text: [AO] Arctic Ocean (MRGID:1906)

text: marine metagenome

characteristic: organism

text: Tara Oceans Expedition (2009-2013)

text: In the size fraction W<0.22 micrometres, the sample material was pre-filtered using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane (ref:GPWP14250) with 0.22-micrometres-pore-size. A volume of 20 Litres was treated with FeCl3, incubated at room temperature for 1-24 hours to allow viruses to precipitate, filtered onto a 1-micrometre-pore-size, 142-mm-diameter GE polycarbonate (PC) membrane filter (ref:K10CP14220) placed on top of a 0.8-micrometre-pore-size, 142-mm-diameter Pall Gelman support filter (ref:60114) using a peristaltic pump with pressure <15 psi. The 1-micrometre-pore-size filter was then packaged in a sterile Falcon tube (50 mL) (up to 3 filters in 1 tube), labelled with a barcode identification sticker and stored on board in a refrigerator at +4 degree Celsius. Note 1: Latex or nitril gloves were used for this protocol. Note 2: All containers, filter holders and tubing were washed with 0.1% bleach, rinsed twice with fresh water and rinsed again twice with the pre-filtered sample material. All tweezers are kept clean with ethanol. Note 3: The three replicates were incubated together. For 20 Litres of filtrate, 2 millilitres of FeCl3 stock solution (4.83 grams of FeCl3-6H2O + 100 millilitres of MilliQ water, stored at 4°C) was added (final concentration of 1 mg Fe per Litre of filtrate), and shaken vigorously for 1 minute. Note 4: Further details about the method are published here doi:10.1111/j.1758-2229.2010.00208.x. In 2009-2012, a total of 3 replicates were prepared and sent for later analysis to the University of Arizona (UA), Tucson, USA. In 2013, a total of 2 replicates were prepared, one sent to for later analysis to the University of Arizona (UA), Tucson, USA, and the other sent for later analysis to the Observatoire Océanologique de Banyuls sur Mer (OOB), France.

text: high volume peristaltic pump [HVP-PUMP]

text: SV Tara

text: marine metagenome

text: TARA_20130718T0827Z_180_EVENT_PUMP_DCM_(30m)_SEQ-(FeCl3)_W<0.22_R110002126

characteristic: title


characteristic: Chlorophyll Sensor

text: Tara Oceans Consortium, Coordinators & Tara Oceans Expedition, Participants. Registry of all samples from the Tara Oceans Expedition (2009-2013). PANGAEA. doi:10.1594/PANGAEA.842197 (2015) (https://dx.doi.org/10.1594/PANGAEA.842197)

characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: GSC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

text: This sample (TARA_N010000840) was collected at station TARA_180 during campaign TARA_20130629Z of the Tara Oceans expedition (2009-2013), using a plankton net [NET-BONGO-180]+[SIEVE-2000]. The sampling event (TARA_20130718T1153Z_180_EVENT_NET) occurred at position latitudeN=74.8237 and longitudeE=76.3349, on date/time=2013-07-18T11:53:00, at a depth of 33-37 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [DCM] deep chlorophyll maximum layer (ENVO:01000326). The sample material was size-fractionated (180-2000 micrometres) and prepared on board using protocol [SEQ-(500mL-or-15min)_N180-2000] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity, metagenomics and/or transcriptomics studies of unicellular eukaryotes (protists). This sample has replicate sample(s): TARA_N010000841, TARA_N010000849, TARA_N010000850.

text: Polar Biome

text: discrete depth within the epipelagic zone (ENVO:00000209) at the interface of the photic zone (ENVO:00000209) and the aphotic zone (ENVO:00000210), coinciding with a strong peak in chlorophyll fluorescence, within <50 m from the sea floor (ENVO:00000482)

text: particulate matter (ENVO:01000060), including planktonic material (ENVO:01000063)

text: water

characteristic: longitude start

text: [AO] Arctic Ocean (MRGID:1906)

characteristic: marine region

characteristic: nitrate sensor

text: marine metagenome

characteristic: oxygen sensor

text: Tara Oceans Expedition (2009-2013)

characteristic: project name

text: In the size fraction N180-2000 micrometres, the sample material was obtained from a net-cod-end collector lined with a nylon mesh of 180-micrometres and passed through a sieve with a metal mesh of 2000-micrometres. Filtered sea water (<0.1-micrometre) was added to the filtrate up to a total volume of 3 Litres. A volume of 500 mL from the diluted filtrate was filtered in parallel on two 10-micrometres-pore-size, 47-mm-diameter Millipore polycarbonate membrane filters (ref:TCTP04700), packaged in a sterile cryogenic tube (5 mL) (2 filters in 1 tube), labelled with a barcode identification sticker and stored on board in liquid nitrogen. Note 1: Latex or nitril gloves were used for this protocol. Note 2: All containers, filter holders and tubing were washed with 0.1% bleach, rinsed twice with fresh water and rinsed again twice with the pre-filtered sample material. All tweezers are kept clean with ethanol. Note 3: The entire process did not exceed 15 minutes. A total of 2 replicates were prepared and sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

characteristic: protocol description

characteristic: protocol label

characteristic: sample collection device

characteristic: sample material label

characteristic: sample status

characteristic: sampling campaign

text: SV Tara

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: TARA_20130718T1153Z_180_EVENT_NET_DCM_(35m)_SEQ-(500mL-or-15min)_N180-2000_N010000840


characteristic: Chlorophyll Sensor

characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: GSC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (TARA_N010000851) was collected at station TARA_180 during campaign TARA_20130629Z of the Tara Oceans expedition (2009-2013), using a plankton net [NET-DOUBLE-20]+[SIEVE-180]. The sampling event (TARA_20130718T1223Z_180_EVENT_NET) occurred at position latitudeN=74.8203 and longitudeE=76.2723, on date/time=2013-07-18T12:23:00, at a depth of 28-32 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [DCM] deep chlorophyll maximum layer (ENVO:01000326). The sample material was size-fractionated (20-180 micrometres) and prepared on board using protocol [SEQ-(500mL-or-15min)_N20-180] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity, metagenomics and/or transcriptomics studies of unicellular eukaryotes (protists). This sample has replicate sample(s): TARA_N010000842, TARA_N010000843, TARA_N010000848.

characteristic: description

text: Polar Biome

characteristic: environment (biome)

characteristic: environment (feature)

text: discrete depth within the epipelagic zone (ENVO:00000209) at the interface of the photic zone (ENVO:00000209) and the aphotic zone (ENVO:00000210), <10 m from a strong peak in chlorophyll fluorescence, within <50 m from the sea floor (ENVO:00000482)

characteristic: environment (feature) further details

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

characteristic: event label

characteristic: further details

characteristic: last update date

characteristic: latitude start

text: [AO] Arctic Ocean (MRGID:1906)

text: marine metagenome

characteristic: organism

text: Tara Oceans Expedition (2009-2013)

text: In the size fraction N20-180 micrometres, the sample material was obtained from a net-cod-end collector lined with a nylon mesh of 20-micrometres and passed through a sieve with a metal mesh of 180-micrometres. Filtered sea water (<0.1-micrometre) was added to the filtrate up to a total volume of 3 Litres. A volume of 500 mL from the diluted filtrate was filtered in parallel on two 10-micrometres-pore-size, 47-mm-diameter Millipore polycarbonate membrane filters (ref:TCTP04700), packaged in a sterile cryogenic tube (5 mL) (2 filters in 1 tube), labelled with a barcode identification sticker and stored on board in liquid nitrogen. Note 1: Latex or nitril gloves were used for this protocol. Note 2: All containers, filter holders and tubing were washed with 0.1% bleach, rinsed twice with fresh water and rinsed again twice with the pre-filtered sample material. All tweezers are kept clean with ethanol. Note 3: The entire process did not exceed 15 minutes. A total of 2 replicates were prepared and sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

text: SV Tara

text: marine metagenome

text: TARA_20130718T1223Z_180_EVENT_NET_DCM_(30m)_SEQ-(500mL-or-15min)_N20-180_N010000851

characteristic: title