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characteristic: INSDC center name

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text: CTGCTGCTG

characteristic: multiplex identifiers

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characteristic: project name

text: uncultured fungus

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text: Fungal ITS region


status: PUBLIC

tag: attribute

characteristic: ENA-FIRST-PUBLIC

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text: uncultured fungus

text: biofilm from titanium alloy (Ti-6A1-4V) coated carbon steel surface

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characteristic: environment (biome)

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characteristic: geographic location (country and/or sea)

unit: DD

characteristic: geographic location (latitude)

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characteristic: investigation type

text: miscellaneous natural or artificial environment

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text: ACTGTGCAG

text: uncultured fungus

characteristic: organism

text: AGCTGCGTTCTTCATCGATGC,CTTGGTCATTTAGAGGAAGTA

text: uncultured fungus

text: Ion Torrent

characteristic: sequencing method

text: Fungal ITS region

characteristic: target gene

characteristic: target subfragment

characteristic: title

type: has member


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characteristic: SRA accession

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characteristic: geographic location (country and/or sea)

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characteristic: investigation type

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text: CGACACTCG

text: uncultured fungus

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text: uncultured fungus

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characteristic: sequencing method

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text: biofilm from 454SMO carbon steel surface

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characteristic: target gene

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characteristic: geographic location (country and/or sea)

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characteristic: investigation type

text: TCGCTCGCG

characteristic: multiplex identifiers

text: uncultured fungus

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characteristic: ENA-CHECKLIST

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characteristic: INSDC center name

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characteristic: miscellaneous environmental package

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text: uncultured fungus

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characteristic: sequencing method

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characteristic: ENA-FIRST-PUBLIC

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text: VTT

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characteristic: collection date

text: uncultured fungus

text: biofilm from epoxy (Intershield Inerta 160) coated carbon steel surface

text: biofilm

characteristic: environment (biome)

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text: water

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text: Finland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: AGAGAGAGC

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text: uncultured fungus

text: Ion Torrent

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characteristic: target gene

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characteristic: SRA accession

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characteristic: ENA-FIRST-PUBLIC

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characteristic: geographic location (country and/or sea)

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text: mimarks-survey

characteristic: investigation type

characteristic: miscellaneous environmental package

text: CGATCGATC

text: uncultured fungus

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text: uncultured fungus

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characteristic: sequencing method

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characteristic: ENA-CHECKLIST

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characteristic: SRA accession

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text: uncultured fungus

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text: Brackish water from industrial cooling system

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characteristic: target gene

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text: VTT

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characteristic: geographic location (country and/or sea)

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characteristic: ENA-CHECKLIST

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characteristic: SRA accession

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text: uncultured fungus

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text: Brackish chlorinated water from industrial cooling system

characteristic: description

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characteristic: miscellaneous environmental package

text: CACACTCTC

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text: uncultured fungus

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characteristic: sequencing method

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characteristic: geographic location (country and/or sea)

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text: mimarks-survey

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text: ACACTAGAC

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characteristic: ENA-FIRST-PUBLIC

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characteristic: environment (biome)

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characteristic: geographic location (country and/or sea)

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text: mimarks-survey

characteristic: investigation type

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text: CTAGTGCGC

text: uncultured fungus

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characteristic: geographic location (country and/or sea)

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text: mimarks-survey

characteristic: investigation type

text: TGCTCGTCT

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text: uncultured fungus

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characteristic: ENA-CHECKLIST

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text: VTT

characteristic: INSDC center name

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text: uncultured fungus

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text: seawater

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characteristic: miscellaneous environmental package

text: CAGCAGCAG

text: uncultured fungus

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text: uncultured fungus

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characteristic: sequencing method

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characteristic: ENA-FIRST-PUBLIC

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characteristic: environment (biome)

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characteristic: geographic location (country and/or sea)

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text: mimarks-survey

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text: ATGCATGCT

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characteristic: ENA-FIRST-PUBLIC

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characteristic: geographic location (country and/or sea)

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text: mimarks-survey

characteristic: investigation type

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text: CTGCATCGC

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characteristic: sequencing method

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characteristic: ENA-CHECKLIST

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text: uncultured bacterium

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text: 454SMO carbon steel surface

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text: ACTGACTGC

text: uncultured bacterium

text: ACCTACGGGNGGCWGCAG,GACTACHVGGGTATCTAATCC

text: uncultured bacterium

text: Ion Torrent

text: Bacterial 16S rRNA gene

characteristic: target gene

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characteristic: environment (biome)

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text: water

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characteristic: geographic location (country and/or sea)

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text: mimarks-survey

characteristic: investigation type

text: TACGTACGT

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text: uncultured bacterium

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text: Bacterial 16S rRNA gene


characteristic: ENA-CHECKLIST

characteristic: External Id

text: VTT

characteristic: INSDC center name

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text: uncultured bacterium

characteristic: common name

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characteristic: miscellaneous environmental package

text: TCAGTCAGT

text: uncultured bacterium

characteristic: organism

text: uncultured bacterium

text: Ion Torrent

characteristic: sequencing method

text: Bacterial 16S rRNA gene


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text: VTT

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characteristic: collection date

text: uncultured bacterium

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characteristic: environment (biome)

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text: water

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text: Finland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: ATCTGTATC

text: uncultured bacterium

text: uncultured bacterium

text: Ion Torrent

text: Bacterial 16S rRNA gene

characteristic: target gene

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text: VTT

characteristic: INSDC center name

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characteristic: SRA accession

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text: uncultured bacterium

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characteristic: multiplex identifiers

text: uncultured bacterium

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characteristic: ENA-FIRST-PUBLIC

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text: VTT

text: public

characteristic: collection date

text: uncultured bacterium

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characteristic: environment (biome)

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text: Finland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

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text: mimarks-survey

characteristic: investigation type

characteristic: miscellaneous environmental package

text: TCTAGCGAG

text: uncultured bacterium

characteristic: organism

text: uncultured bacterium

text: Ion Torrent

characteristic: sequencing method

text: Bacterial 16S rRNA gene


characteristic: ENA-CHECKLIST

characteristic: External Id

text: VTT

characteristic: INSDC center name

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text: uncultured bacterium

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text: public

characteristic: collection date

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characteristic: environment (biome)

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text: water

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text: Finland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

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text: mimarks-survey

characteristic: investigation type

text: CTCAGCAGT

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text: uncultured bacterium

characteristic: pcr primers

characteristic: project name

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text: Bacterial 16S rRNA gene


characteristic: ENA-CHECKLIST

characteristic: External Id

text: VTT

characteristic: INSDC center name

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text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: uncultured bacterium

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text: CTGTCATAG

text: uncultured bacterium

characteristic: organism

text: uncultured bacterium

text: Ion Torrent

characteristic: sequencing method

text: Bacterial 16S rRNA gene


characteristic: ENA-FIRST-PUBLIC

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text: VTT

text: public

characteristic: collection date

text: uncultured bacterium

text: biofilm

characteristic: environment (biome)

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text: water

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text: Finland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: CTCAGTGAT

text: uncultured bacterium

text: uncultured bacterium

text: Ion Torrent

text: Bacterial 16S rRNA gene

characteristic: target gene

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characteristic: External Id

text: VTT

characteristic: INSDC center name

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characteristic: SRA accession

characteristic: Submitter Id

text: uncultured bacterium

characteristic: common name

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text: 454SMO carbon steel surface

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text: Finland

text: mimarks-survey

text: TGTAGTGAG

characteristic: multiplex identifiers

text: uncultured bacterium

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characteristic: project name

text: uncultured bacterium

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text: Bacterial 16S rRNA gene


characteristic: ENA-FIRST-PUBLIC

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text: VTT

text: public

characteristic: collection date

text: uncultured bacterium

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characteristic: environment (biome)

text: 454SMO carbon steel surface

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text: water

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text: Finland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

characteristic: miscellaneous environmental package

text: CACGTATGT

text: uncultured bacterium

characteristic: organism

text: uncultured bacterium

text: Ion Torrent

characteristic: sequencing method

text: Bacterial 16S rRNA gene


characteristic: ENA-CHECKLIST

characteristic: External Id

text: VTT

characteristic: INSDC center name

characteristic: INSDC first public

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characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: uncultured bacterium

characteristic: common name

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text: biofilm

text: 454SMO carbon steel surface

text: water

text: Finland

text: mimarks-survey

text: CAGATAGAT

text: uncultured bacterium

text: uncultured bacterium

text: Ion Torrent

text: Bacterial 16S rRNA gene

characteristic: target gene

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text: VTT

text: public

characteristic: collection date

text: uncultured bacterium

text: biofilm

characteristic: environment (biome)

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text: water

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text: Finland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: CACTGAGTG

characteristic: multiplex identifiers

text: uncultured bacterium

characteristic: pcr primers

characteristic: project name

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text: Bacterial 16S rRNA gene


characteristic: ENA-CHECKLIST

characteristic: External Id

text: VTT

characteristic: INSDC center name

characteristic: INSDC first public

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characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: uncultured bacterium

characteristic: common name

characteristic: description

text: biofilm

text: water

text: Finland

text: mimarks-survey

characteristic: miscellaneous environmental package

text: CAGCGAGTC

text: uncultured bacterium

characteristic: organism

text: uncultured bacterium

text: Ion Torrent

characteristic: sequencing method

text: Bacterial 16S rRNA gene


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: VTT

text: public

characteristic: collection date

text: uncultured bacterium

text: biofilm

characteristic: environment (biome)

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text: water

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text: Finland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: CATAGATCG

text: uncultured bacterium

text: uncultured bacterium

text: Ion Torrent

text: Bacterial 16S rRNA gene

characteristic: target gene

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characteristic: ENA-CHECKLIST

characteristic: External Id

text: VTT

characteristic: INSDC center name

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text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: uncultured bacterium

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text: 454SMO carbon steel surface

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text: Finland

text: mimarks-survey

text: CTAGCGTAC

characteristic: multiplex identifiers

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characteristic: project name

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characteristic: ENA-FIRST-PUBLIC

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text: VTT

text: public

characteristic: collection date

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text: water

characteristic: environment (material)

text: Finland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

characteristic: miscellaneous environmental package

text: CTCTGACAT

text: uncultured bacterium

characteristic: organism

text: uncultured bacterium

text: Ion Torrent

characteristic: sequencing method

text: Bacterial 16S rRNA gene


characteristic: ENA-CHECKLIST

characteristic: External Id

text: VTT

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: uncultured bacterium

characteristic: common name

characteristic: description

text: biofilm

text: 454SMO carbon steel surface

text: water

text: Finland

text: mimarks-survey

text: CTGATCTGT

text: uncultured bacterium

text: uncultured bacterium

text: Ion Torrent

text: Bacterial 16S rRNA gene

characteristic: target gene

characteristic: target subfragment

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: VTT

text: public

characteristic: collection date

text: uncultured bacterium

text: biofilm

characteristic: environment (biome)

characteristic: environment (feature)

text: water

characteristic: environment (material)

text: Finland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: ACAGTCTGT

characteristic: multiplex identifiers

text: uncultured bacterium

characteristic: pcr primers

characteristic: project name

text: uncultured bacterium

text: Ion Torrent

text: Bacterial 16S rRNA gene


characteristic: ENA-CHECKLIST

characteristic: External Id

text: VTT

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: uncultured bacterium

characteristic: common name

characteristic: description

text: biofilm

text: water

text: Finland

text: mimarks-survey

characteristic: miscellaneous environmental package

text: ACACTGTCG

text: uncultured bacterium

characteristic: organism

text: uncultured bacterium

text: Ion Torrent

characteristic: sequencing method

text: Bacterial 16S rRNA gene


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: VTT

text: public

characteristic: collection date

text: uncultured bacterium

text: biofilm

characteristic: environment (biome)

characteristic: environment (feature)

text: water

characteristic: environment (material)

text: Finland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: AGATCGCAC

text: uncultured bacterium

text: uncultured bacterium

text: Ion Torrent

text: Bacterial 16S rRNA gene

characteristic: target gene

characteristic: target subfragment

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: External Id

text: VTT

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: uncultured bacterium

characteristic: common name

characteristic: description

text: biofilm

text: water

text: Finland

text: mimarks-survey

text: AGCATCGAG

characteristic: multiplex identifiers

text: uncultured bacterium

characteristic: pcr primers

characteristic: project name

text: uncultured bacterium

text: Ion Torrent

text: Bacterial 16S rRNA gene


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: VTT

text: public

characteristic: collection date

text: uncultured bacterium

text: biofilm

characteristic: environment (biome)

characteristic: environment (feature)

text: water

characteristic: environment (material)

text: Finland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

characteristic: miscellaneous environmental package

text: ACGATCGTG

text: uncultured bacterium

characteristic: organism

text: uncultured bacterium

text: Ion Torrent

characteristic: sequencing method

text: Bacterial 16S rRNA gene


characteristic: ENA-CHECKLIST

characteristic: External Id

text: VTT

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: uncultured bacterium

characteristic: common name

characteristic: description

text: biofilm

text: water

text: Finland

text: mimarks-survey

text: TCGCACTAG

text: uncultured bacterium

text: uncultured bacterium

text: Ion Torrent

text: Bacterial 16S rRNA gene

characteristic: target gene

characteristic: target subfragment

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: VTT

text: public

characteristic: collection date

text: uncultured bacterium

text: biofilm

characteristic: environment (biome)

characteristic: environment (feature)

text: water

characteristic: environment (material)

text: Finland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: TAGACTCTG

characteristic: multiplex identifiers

text: uncultured bacterium

characteristic: pcr primers

characteristic: project name

text: uncultured bacterium

text: Ion Torrent

text: Bacterial 16S rRNA gene


characteristic: ENA-CHECKLIST

characteristic: External Id

text: VTT

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: uncultured bacterium

characteristic: common name

characteristic: description

text: biofilm

text: water

text: Finland

text: mimarks-survey

characteristic: miscellaneous environmental package

text: TAGCTGTAC

text: uncultured bacterium

characteristic: organism

text: uncultured bacterium

text: Ion Torrent

characteristic: sequencing method

text: Bacterial 16S rRNA gene


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: VTT

text: public

characteristic: collection date

text: uncultured bacterium

text: biofilm

characteristic: environment (biome)

characteristic: environment (feature)

text: water

characteristic: environment (material)

text: Finland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: TACAGTGAC

text: uncultured bacterium

text: uncultured bacterium

text: Ion Torrent

text: Bacterial 16S rRNA gene

characteristic: target gene

characteristic: target subfragment

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: External Id

text: VTT

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: uncultured bacterium

characteristic: common name

characteristic: description

text: biofilm

text: 454SMO carbon steel surface

text: water

text: Finland

text: mimarks-survey

text: TCTGACAGC

characteristic: multiplex identifiers

text: uncultured bacterium

characteristic: pcr primers

characteristic: project name

text: uncultured bacterium

text: Ion Torrent

text: Bacterial 16S rRNA gene


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: VTT

text: public

characteristic: collection date

text: uncultured bacterium

text: biofilm

characteristic: environment (biome)

text: 454SMO carbon steel surface

characteristic: environment (feature)

text: water

characteristic: environment (material)

text: Finland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

characteristic: miscellaneous environmental package

text: TCACGTCTG

text: uncultured bacterium

characteristic: organism

text: uncultured bacterium

text: Ion Torrent

characteristic: sequencing method

text: Bacterial 16S rRNA gene


characteristic: ENA-CHECKLIST

characteristic: External Id

text: VTT

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: uncultured bacterium

characteristic: common name

characteristic: description

text: biofilm

text: 454SMO carbon steel surface

text: water

text: Finland

text: mimarks-survey

text: ATGATGATG

text: uncultured bacterium

text: uncultured bacterium

text: Ion Torrent

text: Bacterial 16S rRNA gene

characteristic: target gene

characteristic: target subfragment

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: VTT

text: public

characteristic: collection date

text: uncultured bacterium

text: biofilm

characteristic: environment (biome)

characteristic: environment (feature)

text: water

characteristic: environment (material)

text: Finland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: CTACTACTA

characteristic: multiplex identifiers

text: uncultured bacterium

characteristic: pcr primers

characteristic: project name

text: uncultured bacterium

text: Ion Torrent

text: Bacterial 16S rRNA gene


characteristic: ENA-CHECKLIST

characteristic: External Id

text: VTT

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: uncultured bacterium

characteristic: common name

characteristic: description

text: biofilm

text: water

text: Finland

text: mimarks-survey

characteristic: miscellaneous environmental package

text: TAGCTAGCT

text: uncultured bacterium

characteristic: organism

text: uncultured bacterium

text: Ion Torrent

characteristic: sequencing method

text: Bacterial 16S rRNA gene


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: VTT

text: public

characteristic: collection date

text: uncultured bacterium

text: biofilm

characteristic: environment (biome)

characteristic: environment (feature)

text: water

characteristic: environment (material)

text: Finland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: AGCTAGCTC

text: uncultured bacterium

text: uncultured bacterium

text: Ion Torrent

text: Bacterial 16S rRNA gene

characteristic: target gene

characteristic: target subfragment

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: External Id

text: VTT

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: uncultured bacterium

characteristic: common name

characteristic: description

text: seawater

text: Baltic Sea

text: water

text: Finland

text: mimarks-survey

text: CAGTCAGTC

characteristic: multiplex identifiers

text: uncultured bacterium

characteristic: pcr primers

characteristic: project name

text: uncultured bacterium

text: Ion Torrent

text: Bacterial 16S rRNA gene


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: VTT

text: public

characteristic: collection date

text: uncultured bacterium

text: seawater

characteristic: environment (biome)

text: Baltic Sea

characteristic: environment (feature)

text: water

characteristic: environment (material)

text: Finland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

characteristic: miscellaneous environmental package

text: ATCGCTAGT

text: uncultured bacterium

characteristic: organism

text: uncultured bacterium

text: Ion Torrent

characteristic: sequencing method

text: Bacterial 16S rRNA gene


characteristic: ENA-CHECKLIST

characteristic: External Id

text: VTT

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: uncultured bacterium

characteristic: common name

characteristic: description

text: seawater

text: Baltic Sea

text: water

text: Finland

text: mimarks-survey

text: CGATAGATC

text: uncultured bacterium

text: uncultured bacterium

text: Ion Torrent

text: Bacterial 16S rRNA gene

characteristic: target gene

characteristic: target subfragment

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: VTT

text: public

characteristic: collection date

text: uncultured bacterium

text: seawater

characteristic: environment (biome)

text: Baltic Sea

characteristic: environment (feature)

text: water

characteristic: environment (material)

text: Finland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: TGTCGATAG

characteristic: multiplex identifiers

text: uncultured bacterium

characteristic: pcr primers

characteristic: project name

text: uncultured bacterium

text: Ion Torrent

text: Bacterial 16S rRNA gene


characteristic: ENA-CHECKLIST

characteristic: External Id

text: VTT

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: uncultured bacterium

characteristic: common name

characteristic: description

text: seawater

text: Baltic Sea

text: water

text: Finland

text: mimarks-survey

characteristic: miscellaneous environmental package

text: CGAGCGAGT

text: uncultured bacterium

characteristic: organism

text: uncultured bacterium

text: Ion Torrent

characteristic: sequencing method

text: Bacterial 16S rRNA gene


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: VTT

text: public

characteristic: collection date

text: uncultured bacterium

text: seawater

characteristic: environment (biome)

text: Baltic Sea

characteristic: environment (feature)

text: water

characteristic: environment (material)

text: Finland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: TGTCTAGCG

text: uncultured bacterium

text: uncultured bacterium

text: Ion Torrent

text: Bacterial 16S rRNA gene

characteristic: target gene

characteristic: target subfragment

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: External Id

text: VTT

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: uncultured bacterium

characteristic: common name

characteristic: description

text: seawater

text: Baltic Sea

text: water

text: Finland

text: mimarks-survey

text: ACGCATAGT

characteristic: multiplex identifiers

text: uncultured bacterium

characteristic: pcr primers

characteristic: project name

text: uncultured bacterium

text: Ion Torrent

text: Bacterial 16S rRNA gene


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: VTT

text: public

characteristic: collection date

text: uncultured bacterium

text: seawater

characteristic: environment (biome)

text: Baltic Sea

characteristic: environment (feature)

text: water

characteristic: environment (material)

text: Finland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

characteristic: miscellaneous environmental package

text: TGACTGACT

text: uncultured bacterium

characteristic: organism

text: uncultured bacterium

text: Ion Torrent

characteristic: sequencing method

text: Bacterial 16S rRNA gene


characteristic: ENA-CHECKLIST

characteristic: External Id

text: VTT

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: uncultured bacterium

characteristic: common name

characteristic: description

text: seawater

text: Baltic Sea

text: water

text: Finland

text: mimarks-survey

text: CAGTCAGCG

text: uncultured bacterium

text: uncultured bacterium

text: Ion Torrent

text: Bacterial 16S rRNA gene

characteristic: target gene

characteristic: target subfragment

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: VTT

text: public

characteristic: collection date

text: uncultured bacterium

text: seawater

characteristic: environment (biome)

text: Baltic Sea

characteristic: environment (feature)

text: water

characteristic: environment (material)

text: Finland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: AGACAGACT

characteristic: multiplex identifiers

text: uncultured bacterium

characteristic: pcr primers

characteristic: project name

text: uncultured bacterium

text: Ion Torrent

text: Bacterial 16S rRNA gene


characteristic: ENA-CHECKLIST

characteristic: External Id

text: VTT

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: uncultured bacterium

characteristic: common name

characteristic: description

text: seawater

text: Baltic Sea

text: water

text: Finland

text: mimarks-survey

characteristic: miscellaneous environmental package

text: TGTGTGTGC

text: uncultured bacterium

characteristic: organism

text: uncultured bacterium

text: Ion Torrent

characteristic: sequencing method

text: Bacterial 16S rRNA gene


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: VTT

text: public

characteristic: collection date

text: uncultured bacterium

text: seawater

characteristic: environment (biome)

text: Baltic Sea

characteristic: environment (feature)

text: water

characteristic: environment (material)

text: Finland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: CACACGCGT

text: uncultured bacterium

text: uncultured bacterium

text: Ion Torrent

text: Bacterial 16S rRNA gene

characteristic: target gene

characteristic: target subfragment

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: External Id

text: VTT

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: uncultured archaeon

characteristic: common name

characteristic: description

text: biofilm

text: 454SMO carbon steel surface

text: water

text: Finland

text: mimarks-survey

text: AGTGCAGAT

characteristic: multiplex identifiers

text: uncultured archaeon

text: AGYGCASCAGKCGMGAAW,GGACTACVSGGGTATCTAAT

characteristic: pcr primers

characteristic: project name

text: uncultured archaeon

text: Ion Torrent

text: Archaeal 16S rRNA gene


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: VTT

text: public

characteristic: collection date

text: uncultured archaeon

text: biofilm

characteristic: environment (biome)

text: 454SMO carbon steel surface

characteristic: environment (feature)

text: water

characteristic: environment (material)

text: Finland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

characteristic: miscellaneous environmental package

text: TACTGACTG

text: uncultured archaeon

characteristic: organism

text: AGYGCASCAGKCGMGAAW,GGACTACVSGGGTATCTAAT

text: uncultured archaeon

text: Ion Torrent

characteristic: sequencing method

text: Archaeal 16S rRNA gene


characteristic: ENA-CHECKLIST

characteristic: External Id

text: VTT

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: uncultured archaeon

characteristic: common name

characteristic: description

text: biofilm

text: 454SMO carbon steel surface

text: water

text: Finland

text: mimarks-survey

text: CTGTGACAG

text: uncultured archaeon

text: AGYGCASCAGKCGMGAAW,GGACTACVSGGGTATCTAAT

text: uncultured archaeon

text: Ion Torrent

text: Archaeal 16S rRNA gene

characteristic: target gene

characteristic: target subfragment

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: VTT

text: public

characteristic: collection date

text: uncultured archaeon

text: biofilm

characteristic: environment (biome)

characteristic: environment (feature)

text: water

characteristic: environment (material)

text: Finland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: ACATCGACG

characteristic: multiplex identifiers

text: uncultured archaeon

text: AGYGCASCAGKCGMGAAW,GGACTACVSGGGTATCTAAT

characteristic: pcr primers

characteristic: project name

text: uncultured archaeon

text: Ion Torrent

text: Archaeal 16S rRNA gene


characteristic: ENA-CHECKLIST

characteristic: External Id

text: VTT

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: uncultured archaeon

characteristic: common name

characteristic: description

text: biofilm

text: water

text: Finland

text: mimarks-survey

characteristic: miscellaneous environmental package

text: TCTCACACG

text: uncultured archaeon

characteristic: organism

text: AGYGCASCAGKCGMGAAW,GGACTACVSGGGTATCTAAT

text: uncultured archaeon

text: Ion Torrent

characteristic: sequencing method

text: Archaeal 16S rRNA gene


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: VTT

text: public

characteristic: collection date

text: uncultured archaeon

text: biofilm

characteristic: environment (biome)

characteristic: environment (feature)

text: water

characteristic: environment (material)

text: Finland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: CGATCAGTG

text: uncultured archaeon

text: AGYGCASCAGKCGMGAAW,GGACTACVSGGGTATCTAAT

text: uncultured archaeon

text: Ion Torrent

text: Archaeal 16S rRNA gene

characteristic: target gene

characteristic: target subfragment

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: External Id

text: VTT

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: uncultured archaeon

characteristic: common name

characteristic: description

text: biofilm

text: water

text: Finland

text: mimarks-survey

text: AGACAGATG

characteristic: multiplex identifiers

text: uncultured archaeon

text: AGYGCASCAGKCGMGAAW,GGACTACVSGGGTATCTAAT

characteristic: pcr primers

characteristic: project name

text: uncultured archaeon

text: Ion Torrent

text: Archaeal 16S rRNA gene


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: VTT

text: public

characteristic: collection date

text: uncultured archaeon

text: biofilm

characteristic: environment (biome)

characteristic: environment (feature)

text: water

characteristic: environment (material)

text: Finland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

characteristic: miscellaneous environmental package

text: TGTACGCAG

text: uncultured archaeon

characteristic: organism

text: AGYGCASCAGKCGMGAAW,GGACTACVSGGGTATCTAAT

text: uncultured archaeon

text: Ion Torrent

characteristic: sequencing method

text: Archaeal 16S rRNA gene


characteristic: ENA-CHECKLIST

characteristic: External Id

text: VTT

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: uncultured archaeon

characteristic: common name

characteristic: description

text: biofilm

text: water

text: Finland

text: mimarks-survey

text: CGATCAGTC

text: uncultured archaeon

text: AGYGCASCAGKCGMGAAW,GGACTACVSGGGTATCTAAT

text: uncultured archaeon

text: Ion Torrent

text: Archaeal 16S rRNA gene

characteristic: target gene

characteristic: target subfragment

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: VTT

text: public

characteristic: collection date

text: uncultured archaeon

text: biofilm

characteristic: environment (biome)

characteristic: environment (feature)

text: water

characteristic: environment (material)

text: Finland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: AGCATGCAC

characteristic: multiplex identifiers

text: uncultured archaeon

text: AGYGCASCAGKCGMGAAW,GGACTACVSGGGTATCTAAT

characteristic: pcr primers

characteristic: project name

text: uncultured archaeon

text: Ion Torrent

text: Archaeal 16S rRNA gene


characteristic: ENA-CHECKLIST

characteristic: External Id

text: VTT

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: uncultured archaeon

characteristic: common name

characteristic: description

text: biofilm

text: water

text: Finland

text: mimarks-survey

characteristic: miscellaneous environmental package

text: TCAGACTAG

text: uncultured archaeon

characteristic: organism

text: AGYGCASCAGKCGMGAAW,GGACTACVSGGGTATCTAAT

text: uncultured archaeon

text: Ion Torrent

characteristic: sequencing method

text: Archaeal 16S rRNA gene


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: VTT

text: public

characteristic: collection date

text: uncultured archaeon

text: biofilm

characteristic: environment (biome)

characteristic: environment (feature)

text: water

characteristic: environment (material)

text: Finland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: CTGACTGTC

text: uncultured archaeon

text: AGYGCASCAGKCGMGAAW,GGACTACVSGGGTATCTAAT

text: uncultured archaeon

text: Ion Torrent

text: Archaeal 16S rRNA gene

characteristic: target gene

characteristic: target subfragment

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: External Id

text: VTT

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: uncultured archaeon

characteristic: common name

characteristic: description

text: biofilm

text: 454SMO carbon steel surface

text: water

text: Finland

text: mimarks-survey

text: AGTACTGAG

characteristic: multiplex identifiers

text: uncultured archaeon

text: AGYGCASCAGKCGMGAAW,GGACTACVSGGGTATCTAAT

characteristic: pcr primers

characteristic: project name

text: uncultured archaeon

text: Ion Torrent

text: Archaeal 16S rRNA gene


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: VTT

text: public

characteristic: collection date

text: uncultured archaeon

text: biofilm

characteristic: environment (biome)

text: 454SMO carbon steel surface

characteristic: environment (feature)

text: water

characteristic: environment (material)

text: Finland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

characteristic: miscellaneous environmental package

text: TCACATGCG

text: uncultured archaeon

characteristic: organism

text: AGYGCASCAGKCGMGAAW,GGACTACVSGGGTATCTAAT

text: uncultured archaeon

text: Ion Torrent

characteristic: sequencing method

text: Archaeal 16S rRNA gene


characteristic: ENA-CHECKLIST

characteristic: External Id

text: VTT

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: uncultured archaeon

characteristic: common name

characteristic: description

text: biofilm

text: 454SMO carbon steel surface

text: water

text: Finland

text: mimarks-survey

text: CGTACAGTG

text: uncultured archaeon

text: AGYGCASCAGKCGMGAAW,GGACTACVSGGGTATCTAAT

text: uncultured archaeon

text: Ion Torrent

text: Archaeal 16S rRNA gene

characteristic: target gene

characteristic: target subfragment

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: VTT

text: public

characteristic: collection date

text: uncultured archaeon

text: biofilm

characteristic: environment (biome)

characteristic: environment (feature)

text: water

characteristic: environment (material)

text: Finland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: ATCGCATAC

characteristic: multiplex identifiers

text: uncultured archaeon

text: AGYGCASCAGKCGMGAAW,GGACTACVSGGGTATCTAAT

characteristic: pcr primers

characteristic: project name

text: uncultured archaeon

text: Ion Torrent

text: Archaeal 16S rRNA gene


characteristic: ENA-CHECKLIST

characteristic: External Id

text: VTT

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: uncultured archaeon

characteristic: common name

characteristic: description

text: biofilm

text: water

text: Finland

text: mimarks-survey

characteristic: miscellaneous environmental package

text: TGTCTAGCG

text: uncultured archaeon

characteristic: organism

text: AGYGCASCAGKCGMGAAW,GGACTACVSGGGTATCTAAT

text: uncultured archaeon

text: Ion Torrent

characteristic: sequencing method

text: Archaeal 16S rRNA gene


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: VTT

text: public

characteristic: collection date

text: uncultured archaeon

text: biofilm

characteristic: environment (biome)

characteristic: environment (feature)

text: water

characteristic: environment (material)

text: Finland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: CTCTCGATG

text: uncultured archaeon

text: AGYGCASCAGKCGMGAAW,GGACTACVSGGGTATCTAAT

text: uncultured archaeon

text: Ion Torrent

text: Archaeal 16S rRNA gene

characteristic: target gene

characteristic: target subfragment

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: External Id

text: VTT

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: uncultured archaeon

characteristic: common name

characteristic: description

text: biofilm

text: 454SMO carbon steel surface

text: water

text: Finland

text: mimarks-survey

text: ATGCGTAGC

characteristic: multiplex identifiers

text: uncultured archaeon

text: AGYGCASCAGKCGMGAAW,GGACTACVSGGGTATCTAAT

characteristic: pcr primers

characteristic: project name

text: uncultured archaeon

text: Ion Torrent

text: Archaeal 16S rRNA gene


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: VTT

text: public

characteristic: collection date

text: uncultured archaeon

text: biofilm

characteristic: environment (biome)

text: 454SMO carbon steel surface

characteristic: environment (feature)

text: water

characteristic: environment (material)

text: Finland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

characteristic: miscellaneous environmental package

text: TGTGCAGAT

text: uncultured archaeon

characteristic: organism

text: AGYGCASCAGKCGMGAAW,GGACTACVSGGGTATCTAAT

text: uncultured archaeon

text: Ion Torrent

characteristic: sequencing method

text: Archaeal 16S rRNA gene


characteristic: ENA-CHECKLIST

characteristic: External Id

text: VTT

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: uncultured archaeon

characteristic: common name

characteristic: description

text: biofilm

text: 454SMO carbon steel surface

text: water

text: Finland

text: mimarks-survey

text: CTCTATGCG

text: uncultured archaeon

text: AGYGCASCAGKCGMGAAW,GGACTACVSGGGTATCTAAT

text: uncultured archaeon

text: Ion Torrent

text: Archaeal 16S rRNA gene

characteristic: target gene

characteristic: target subfragment

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: VTT

text: public

characteristic: collection date

text: uncultured archaeon

text: biofilm

characteristic: environment (biome)

characteristic: environment (feature)

text: water

characteristic: environment (material)

text: Finland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: ATCAGCGTG

characteristic: multiplex identifiers

text: uncultured archaeon

text: AGYGCASCAGKCGMGAAW,GGACTACVSGGGTATCTAAT

characteristic: pcr primers

characteristic: project name

text: uncultured archaeon

text: Ion Torrent

text: Archaeal 16S rRNA gene


characteristic: ENA-CHECKLIST

characteristic: External Id

text: VTT

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: uncultured archaeon

characteristic: common name

characteristic: description

text: biofilm

text: water

text: Finland

text: mimarks-survey

characteristic: miscellaneous environmental package

text: TGTCAGAGC

text: uncultured archaeon

characteristic: organism

text: AGYGCASCAGKCGMGAAW,GGACTACVSGGGTATCTAAT

text: uncultured archaeon

text: Ion Torrent

characteristic: sequencing method

text: Archaeal 16S rRNA gene


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: VTT

text: public

characteristic: collection date

text: uncultured archaeon

text: biofilm

characteristic: environment (biome)

characteristic: environment (feature)

text: water

characteristic: environment (material)

text: Finland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: CGTCGTAGC

text: uncultured archaeon

text: AGYGCASCAGKCGMGAAW,GGACTACVSGGGTATCTAAT

text: uncultured archaeon

text: Ion Torrent

text: Archaeal 16S rRNA gene

characteristic: target gene

characteristic: target subfragment

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: External Id

text: VTT

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: uncultured archaeon

characteristic: common name

characteristic: description

text: biofilm

text: water

text: Finland

text: mimarks-survey

text: ACTAGCGAC

characteristic: multiplex identifiers

text: uncultured archaeon

text: AGYGCASCAGKCGMGAAW,GGACTACVSGGGTATCTAAT

characteristic: pcr primers

characteristic: project name

text: uncultured archaeon

text: Ion Torrent

text: Archaeal 16S rRNA gene


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: VTT

text: public

characteristic: collection date

text: uncultured archaeon

text: biofilm

characteristic: environment (biome)

characteristic: environment (feature)

text: water

characteristic: environment (material)

text: Finland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

characteristic: miscellaneous environmental package

text: TCTCTGTCG

text: uncultured archaeon

characteristic: organism

text: AGYGCASCAGKCGMGAAW,GGACTACVSGGGTATCTAAT

text: uncultured archaeon

text: Ion Torrent

characteristic: sequencing method

text: Archaeal 16S rRNA gene


characteristic: ENA-CHECKLIST

characteristic: External Id

text: VTT

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: uncultured archaeon

characteristic: common name

characteristic: description

text: biofilm

text: water

text: Finland

text: mimarks-survey

text: CACACAGTG

text: uncultured archaeon

text: AGYGCASCAGKCGMGAAW,GGACTACVSGGGTATCTAAT

text: uncultured archaeon

text: Ion Torrent

text: Archaeal 16S rRNA gene

characteristic: target gene

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characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: VTT

text: public

characteristic: collection date

text: uncultured archaeon

text: biofilm

characteristic: environment (biome)

characteristic: environment (feature)

text: water

characteristic: environment (material)

text: Finland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: AGAGAGCAG

characteristic: multiplex identifiers

text: uncultured archaeon

text: AGYGCASCAGKCGMGAAW,GGACTACVSGGGTATCTAAT

characteristic: pcr primers

characteristic: project name

text: uncultured archaeon

text: Ion Torrent

text: Archaeal 16S rRNA gene


characteristic: ENA-CHECKLIST

characteristic: External Id

text: VTT

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: uncultured archaeon

characteristic: common name

characteristic: description

text: biofilm

text: water

text: Finland

text: mimarks-survey

characteristic: miscellaneous environmental package

text: TGTGTACGC

text: uncultured archaeon

characteristic: organism

text: AGYGCASCAGKCGMGAAW,GGACTACVSGGGTATCTAAT

text: uncultured archaeon

text: Ion Torrent

characteristic: sequencing method

text: Archaeal 16S rRNA gene


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: VTT

text: public

characteristic: collection date

text: uncultured archaeon

text: biofilm

characteristic: environment (biome)

characteristic: environment (feature)

text: water

characteristic: environment (material)

text: Finland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: CTCGCACTG

text: uncultured archaeon

text: AGYGCASCAGKCGMGAAW,GGACTACVSGGGTATCTAAT

text: uncultured archaeon

text: Ion Torrent

text: Archaeal 16S rRNA gene

characteristic: target gene

characteristic: target subfragment

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: External Id

text: VTT

characteristic: INSDC center name

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characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: uncultured archaeon

characteristic: common name

characteristic: description

text: biofilm

text: 454SMO carbon steel surface

text: water

text: Finland

text: mimarks-survey

text: ACACACGCG

characteristic: multiplex identifiers

text: uncultured archaeon

text: AGYGCASCAGKCGMGAAW,GGACTACVSGGGTATCTAAT

characteristic: pcr primers

characteristic: project name

text: uncultured archaeon

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text: Archaeal 16S rRNA gene


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: VTT

text: public

characteristic: collection date

text: uncultured archaeon

text: biofilm

characteristic: environment (biome)

text: 454SMO carbon steel surface

characteristic: environment (feature)

text: water

characteristic: environment (material)

text: Finland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

characteristic: miscellaneous environmental package

text: TGTGACATG

text: uncultured archaeon

characteristic: organism

text: AGYGCASCAGKCGMGAAW,GGACTACVSGGGTATCTAAT

text: uncultured archaeon

text: Ion Torrent

characteristic: sequencing method

text: Archaeal 16S rRNA gene


characteristic: ENA-CHECKLIST

characteristic: External Id

text: VTT

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: uncultured archaeon

characteristic: common name

characteristic: description

text: biofilm

text: 454SMO carbon steel surface

text: water

text: Finland

text: mimarks-survey

text: CTCTCTCTC

text: uncultured archaeon

text: AGYGCASCAGKCGMGAAW,GGACTACVSGGGTATCTAAT

text: uncultured archaeon

text: Ion Torrent

text: Archaeal 16S rRNA gene

characteristic: target gene

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characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: VTT

text: public

characteristic: collection date

text: uncultured archaeon

text: biofilm

characteristic: environment (biome)

characteristic: environment (feature)

text: water

characteristic: environment (material)

text: Finland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: ACACACACC

characteristic: multiplex identifiers

text: uncultured archaeon

text: AGYGCASCAGKCGMGAAW,GGACTACVSGGGTATCTAAT

characteristic: pcr primers

characteristic: project name

text: uncultured archaeon

text: Ion Torrent

text: Archaeal 16S rRNA gene


characteristic: ENA-CHECKLIST

characteristic: External Id

text: VTT

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: uncultured archaeon

characteristic: common name

characteristic: description

text: biofilm

text: water

text: Finland

text: mimarks-survey

characteristic: miscellaneous environmental package

text: TCTCTCTCT

text: uncultured archaeon

characteristic: organism

text: AGYGCASCAGKCGMGAAW,GGACTACVSGGGTATCTAAT

text: uncultured archaeon

text: Ion Torrent

characteristic: sequencing method

text: Archaeal 16S rRNA gene


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: VTT

text: public

characteristic: collection date

text: uncultured archaeon

text: biofilm

characteristic: environment (biome)

characteristic: environment (feature)

text: water

characteristic: environment (material)

text: Finland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: CGATCTCTC

text: uncultured archaeon

text: AGYGCASCAGKCGMGAAW,GGACTACVSGGGTATCTAAT

text: uncultured archaeon

text: Ion Torrent

text: Archaeal 16S rRNA gene

characteristic: target gene

characteristic: target subfragment

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: External Id

text: VTT

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: uncultured archaeon

characteristic: common name

characteristic: description

text: seawater

text: Baltic Sea

text: water

text: Finland

text: mimarks-survey

text: ACGACGATC

characteristic: multiplex identifiers

text: uncultured archaeon

text: AGYGCASCAGKCGMGAAW,GGACTACVSGGGTATCTAAT

characteristic: pcr primers

characteristic: project name

text: uncultured archaeon

text: Ion Torrent

text: Archaeal 16S rRNA gene


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: VTT

text: public

characteristic: collection date

text: uncultured archaeon

text: seawater

characteristic: environment (biome)

text: Baltic Sea

characteristic: environment (feature)

text: water

characteristic: environment (material)

text: Finland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

characteristic: miscellaneous environmental package

text: TGAGTGAGT

text: uncultured archaeon

characteristic: organism

text: AGYGCASCAGKCGMGAAW,GGACTACVSGGGTATCTAAT

text: uncultured archaeon

text: Ion Torrent

characteristic: sequencing method

text: Archaeal 16S rRNA gene


characteristic: ENA-CHECKLIST

characteristic: External Id

text: VTT

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: uncultured archaeon

characteristic: common name

characteristic: description

text: seawater

text: Baltic Sea

text: water

text: Finland

text: mimarks-survey

text: CTCGCTCGC

text: uncultured archaeon

text: AGYGCASCAGKCGMGAAW,GGACTACVSGGGTATCTAAT

text: uncultured archaeon

text: Ion Torrent

text: Archaeal 16S rRNA gene

characteristic: target gene

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characteristic: ENA-LAST-UPDATE

text: VTT

text: public

characteristic: collection date

text: uncultured archaeon

text: seawater

characteristic: environment (biome)

text: Baltic Sea

characteristic: environment (feature)

text: water

characteristic: environment (material)

text: Finland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: AGCGAGCGT

characteristic: multiplex identifiers

text: uncultured archaeon

text: AGYGCASCAGKCGMGAAW,GGACTACVSGGGTATCTAAT

characteristic: pcr primers

characteristic: project name

text: uncultured archaeon

text: Ion Torrent

text: Archaeal 16S rRNA gene


characteristic: ENA-CHECKLIST

characteristic: External Id

text: VTT

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: uncultured archaeon

characteristic: common name

characteristic: description

text: seawater

text: Baltic Sea

text: water

text: Finland

text: mimarks-survey

characteristic: miscellaneous environmental package

text: TCTATCTAG

text: uncultured archaeon

characteristic: organism

text: AGYGCASCAGKCGMGAAW,GGACTACVSGGGTATCTAAT

text: uncultured archaeon

text: Ion Torrent

characteristic: sequencing method

text: Archaeal 16S rRNA gene


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: VTT

text: public

characteristic: collection date

text: uncultured archaeon

text: seawater

characteristic: environment (biome)

text: Baltic Sea

characteristic: environment (feature)

text: water

characteristic: environment (material)

text: Finland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: CTCTCGCAG

text: uncultured archaeon

text: AGYGCASCAGKCGMGAAW,GGACTACVSGGGTATCTAAT

text: uncultured archaeon

text: Ion Torrent

text: Archaeal 16S rRNA gene

characteristic: target gene

characteristic: target subfragment

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: External Id

text: VTT

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: uncultured archaeon

characteristic: common name

characteristic: description

text: seawater

text: Baltic Sea

text: water

text: Finland

text: mimarks-survey

text: AGAGCTCGC

characteristic: multiplex identifiers

text: uncultured archaeon

text: AGYGCASCAGKCGMGAAW,GGACTACVSGGGTATCTAAT

characteristic: pcr primers

characteristic: project name

text: uncultured archaeon

text: Ion Torrent

text: Archaeal 16S rRNA gene


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: VTT

text: public

characteristic: collection date

text: uncultured archaeon

text: seawater

characteristic: environment (biome)

text: Baltic Sea

characteristic: environment (feature)

text: water

characteristic: environment (material)

text: Finland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

characteristic: miscellaneous environmental package

text: TGTAGCGAT

text: uncultured archaeon

characteristic: organism

text: AGYGCASCAGKCGMGAAW,GGACTACVSGGGTATCTAAT

text: uncultured archaeon

text: Ion Torrent

characteristic: sequencing method

text: Archaeal 16S rRNA gene


characteristic: ENA-CHECKLIST

characteristic: External Id

text: VTT

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: uncultured archaeon

characteristic: common name

characteristic: description

text: seawater

text: Baltic Sea

text: water

text: Finland

text: mimarks-survey

text: CACTCACTC

text: uncultured archaeon

text: AGYGCASCAGKCGMGAAW,GGACTACVSGGGTATCTAAT

text: uncultured archaeon

text: Ion Torrent

text: Archaeal 16S rRNA gene

characteristic: target gene

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characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: VTT

text: public

characteristic: collection date

text: uncultured archaeon

text: seawater

characteristic: environment (biome)

text: Baltic Sea

characteristic: environment (feature)

text: water

characteristic: environment (material)

text: Finland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: ACTCACTCG

characteristic: multiplex identifiers

text: uncultured archaeon

text: AGYGCASCAGKCGMGAAW,GGACTACVSGGGTATCTAAT

characteristic: pcr primers

characteristic: project name

text: uncultured archaeon

text: Ion Torrent

text: Archaeal 16S rRNA gene


characteristic: ENA-CHECKLIST

characteristic: External Id

text: VTT

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: uncultured archaeon

characteristic: common name

characteristic: description

text: seawater

text: Baltic Sea

text: water

text: Finland

text: mimarks-survey

characteristic: miscellaneous environmental package

text: TGTGAGAGT

text: uncultured archaeon

characteristic: organism

text: AGYGCASCAGKCGMGAAW,GGACTACVSGGGTATCTAAT

text: uncultured archaeon

text: Ion Torrent

characteristic: sequencing method

text: Archaeal 16S rRNA gene


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: VTT

text: public

characteristic: collection date

text: uncultured archaeon

text: seawater

characteristic: environment (biome)

text: Baltic Sea

characteristic: environment (feature)

text: water

characteristic: environment (material)

text: Finland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: AGCTCGTGT

text: uncultured archaeon

text: AGYGCASCAGKCGMGAAW,GGACTACVSGGGTATCTAAT

text: uncultured archaeon

text: Ion Torrent

text: Archaeal 16S rRNA gene

characteristic: target gene

characteristic: target subfragment

characteristic: title


Exp1 Ctrl1

characteristic: ENA-CHECKLIST

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Exp1 Ctrl1

tag: Namespace:Institut de recherche experimentale et clinique - IREC

characteristic: Submitter Id

text: feces

characteristic: bio material

text: house mouse

characteristic: common name

text: Mus musculus

text: Mus musculus

characteristic: strain

text: Exp1 Ctrl1


Exp1 Ctrl2

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Institut de recherche experimentale et clinique - IREC

text: public

text: Exp1 Ctrl2

text: feces

text: house mouse

text: Mus musculus

characteristic: organism

text: Mus musculus

text: Exp1 Ctrl2


E-MTAB-4778:Sample 1

text: University College London (UCL)

text: public

text: E-MTAB-4778:Sample 1

tag: Namespace:University College London (UCL)

text: ArrayExpress

characteristic: broker name

text: house mouse

text: Protocols: Flow sorted CD8 SP thymocytes Illumina Truseq RNA library preparation kit (Illumina)

characteristic: description

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: Sample 1


E-MTAB-4778:Sample 2

characteristic: ENA-CHECKLIST

text: University College London (UCL)

text: public

text: E-MTAB-4778:Sample 2

text: ArrayExpress

text: house mouse

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: Sample 2

characteristic: title


E-MTAB-4778:Sample 3

characteristic: External Id

text: University College London (UCL)

characteristic: INSDC center name

text: public

text: E-MTAB-4778:Sample 3

text: ArrayExpress

text: house mouse

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: Sample 3


E-MTAB-4778:Sample 4

text: University College London (UCL)

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-4778:Sample 4

text: ArrayExpress

text: house mouse

text: Tnfr1-/- Ikk1 fx/fx Ikk2 fx/fx CD4Cre R26RYFP fx/fx

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: Sample 4


E-MTAB-4778:Sample 5

text: University College London (UCL)

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-4778:Sample 5

text: ArrayExpress

text: house mouse

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: Sample 5


E-MTAB-4778:Sample 6

text: University College London (UCL)

text: public

text: E-MTAB-4778:Sample 6

characteristic: Submitter Id

text: ArrayExpress

text: house mouse

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: Sample 6


E-MTAB-4778:Sample 7

text: University College London (UCL)

text: public

text: E-MTAB-4778:Sample 7

text: ArrayExpress

text: house mouse

characteristic: common name

text: Mus musculus

text: Mus musculus

text: Mus musculus

text: Sample 7


text: The Wadsworth Center

text: public

tag: Namespace:The Wadsworth Center

text: ArrayExpress

text: Protocols: 2mL overnights were grown in LB 37C or 30C at 225rpm and then subcultured 1:100. At an OD 0.5-0.7, RNA was harvested using the hot phenol method. Hot phenol RNA prep: transfer 1mL of subculture to microcentrifuge tube. Spin 1mL at max speed for 1 min and remove supernatant. Resuspend in 0.4mL ethanol+5% acid phenol/chloroform, spin 1 min at max speed and remove supernatant. Resuspend in 1mL 1x TBS, spin 1 min at max speed and remove supernatant. Resuspend in 0.5mL RNA Lysis Buffer (2% SDS, 4mM EDTA) and boil samples for 3 min. Add 0.5mL acid phenol/chloroform to each tube and incubate for 6 min at 65 °C. Incubate 5 min on ice then spin tubes at max speed for 6 min. Transfer upper phase to phase lock tube, add equal volume (0.4mL) chloroform:isoamyl alcohol 24:1 and spin 6 min at max speed. Transfer upper phase to new phase lock tube, add equal volume (0.4mL) chloroform:isoamyl alcohol 24:1 and spin 6 min at max speed. Transfer upper phase to new tube containing 40uL 3M Na Acetate+2uL glycogen (20mg/mL stock). Add 1mL ice cold EtOH and precipitate at -20 °C overnight or 15min at -80 °C. Spin samples for 30 min at 4 °C at max speed. Pipet off supernatant. Wash with 0.7mL of 70% EtOH (in DEPC H2O), spin 5 min at max speed at 4 °C and remove supernatant. Wash again with 0.7mL of 70% EtOH (in DEPC H2O), spin for 5 min at max speed at 4 °C and pipet off supernatant. Dry pellet at room temperature and resuspend in 15uL nuclease free water. DNase treat RNA - set up 50uL reaction including 8-10ug RNA, 5uL of 10X buffer and 1uL DNase enzyme (Ambion). Incubate 30 min at 37 °C. Stop reaction by adding 352uL DEPC H2O + 134uL acid phenol:chloroform + 268uL chloroform:isoamyl alcohol 24:1. Transfer all to phase lock tube and spin at max speed for 6 min. Transfer upper phase to new microcentrifuge tube containing 40uL 3M Na Acetate + 2uL glycogen (20mg/mL stock). Add 1mL ice cold EtOH and precipitate at -20 °C overnight or 15min at -80 °C. Spin samples for 30 min at 4 °C at max speed. Pipet off supernatant. Wash with 0.7mL of 70% EtOH (in DEPC H2O), spin 5 min at max speed at 4 °C and remove supernatant. Wash again with 0.7mL of 70% EtOH (in DEPC H2O), spin for 5 min at max speed at 4 °C and pipet off supernatant. Dry pellet at room temperature and resuspend in 15uL nuclease free water. Remove ribosomal RNA using the RiboZero kit from EpiCentre - wash magnetic beads 2x in RNase free water, then resuspend in Resuspension Solution. Add 32.5uL beads to each tube then add 0.5uL RNase inhibitor. Mix together in final volume of 20uL: 2.5ug DNase treated RNA + 2uL Ribozero reaction buffer + 4uL Ribozero rRNA removal solution. Incubate at 68 °C for 10 min followed by RT for 5 min. Add RNA to beads, mix by pipetting and vortexing for 10 s. Incubate at RT for 5 min. Vortex for 5 s then incubate at 50 °C for 5 min. Place tubes on magnetic stand for 1 min and transfer RNA to new tube. Bring volume to 180uL with DEPC H2O, then add 18uL 3M Na Acetate + 2uL glycogen (20mg/mL stock). Add 0.6mL ice cold EtOH and precipitate at -20 °C overnight. Spin samples for 30 min at 4 °C at max speed. Pipet off supernatant. Wash with 0.7mL of 70% EtOH (in DEPC H2O), spin 5 min at max speed at 4 °C and remove supernatant. Wash again with 0.7mL of 70% EtOH (in DEPC H2O), spin for 5 min at max speed at 4 °C and pipet off supernatant. Dry pellet at room temperature and resuspend in 8uL nuclease free water. Prepare libraries using EpiCentre ScriptSeq v2 kit following kit protocol. Fragment RNA and anneal cDNA synthesis primer in 12uL reaction containing nuclease free water + 40ng rRNA depleted RNA + 1uL RNA Fragmentation solution + 2uL cDNA Synthesis primer. Incubate at 85 °C for 5 min then place tubes on ice. Synthesize DNA by adding 3uL cDNA Synthesis Premix + 0.5uL 100mM DTT + 0.5uL StarScript Reverse Transcriptase. Incubate at 25 °C for 5 min then 42 °C for 20 min. Cool tubes to 37 °C. Add 1uL Finishing solution and incubate at 37 °C for 10 min then 95 °C for 3 min. Cool tubes to 25 °C then add 7.5uL Terminal Tagging Premix + 0.5uL DNA Polymerase to terminally tag the cDNA. Incubate at 25 °C for 15 min, 95 °C for 3 min, then cool to 4 °C. Purify the cDNA using the Qiagen MinElute PCR Purification Kit - Add 5V Buffer PB to sample and transfer all to MinElute column. Spin 1 min at max speed. Discard flow through. Add 0.75mL Buffer PE to column and spin 1 min at max speed. Discard flow through. Spin column for 1 min at max speed then transfer column to new tube. Add 23.4uL Buffer EB to column, incubate 1 min at room temp, then spin 1 min at max speed. Amplify library by setting up 50uL PCR using 22.5uL purified DNA. Add 25uL FailSafe PCR Premix E + 1uL Forward PCR primer + 1uL ScriptSeq Index PCR Primer + 0.5uL FailSafe PCR Enzyme (1.25U). Heat at 95 °C for 1 min, then 12 cycles of 95 °C for 30 sec, 55 °C for 30 sec and 68 °C for 3 min. Incubate at 68 °C for 7 minutes. Purify sample using AMPure XP Purification protocol. Add 50uL beads to each pcr sample. Mix by pipetting 10x. Transfer beads+sample to new tube and incubate at room temp for 15 min. Put tubes on magnetic stand for 5 min then remove supernatant. Wash beads 2x, each time with 0.2mL of 80% EtOH, let stand 1 min then remove supernatant. Air dry beads at room temp for 15 min. Add 12uL nuclease free water, remove tubes from magnetic stand and resuspend beads in water. Incubate at room temp for 2 min. Place tubes on magnetic stand for 5 min. Library is in supernatant. Transfer to new tube. Quantify using Qubit.

text: dhns::kanamycin

text: Escherichia coli str. K-12 substr. MG1655

text: not spiked with Salmonella RNA

characteristic: protocol

unit: Celsius

characteristic: temperature