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text: public

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characteristic: Submitter Id

text: TCAG

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characteristic: collection date

text: Animal facility

characteristic: environment (biome)

text: specific pathogen-free

characteristic: environment (feature)

text: air

characteristic: environment (material)

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: proximal small intestine

text: proximal small intestine

text: house mouse

text: Litter E

text: C57BL/6 wt/wt

text: female

text: host-associated

text: mimarks-survey

characteristic: investigation type

text: ATATCGCGAG

text: mouse gut metagenome

characteristic: organism

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text: mouse gut metagenome

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text: 16S rDNA

text: V1 - V3

text: intestinal microbiota of mouse TRIF_M43_wt, proximal small intestine sample


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text: marine sediments

text: sediments and sedimentary rocks

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characteristic: geographic location (elevation)

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text: uncultured eukaryote

text: Canterbury Basin

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characteristic: environment (biome)

text: Ancient DNA from previous settlement

characteristic: environment (feature)

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text: United Kingdom

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: metagenome

text: uncultured eukaryote

text: Bouldner Cliff sedaDNA

text: uncultured eukaryote

text: sediment

text: Illumina MiSeq

text: Boulder Cliff, sample 1


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text: Boulder Cliff, sample 2


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text: Lib-L Adaptor A primer: CCATCTCATCCCTGCGTGTCTCCGACTCAG. Lib-L Adaptor B Primer: CCTATCCCCTGTGTGCCTTGGCAGTCTCAG.

characteristic: adapters

text: grassland soil

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characteristic: project name

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text: pasture

text: soil

text: United Kingdom

characteristic: geographic location (country and/or sea)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

text: ATAGAGTACT

text: soil metagenome

text: soil metagenome

text: pyrosequencing

characteristic: sequencing method

text: soil

characteristic: soil environmental package

characteristic: soil type

text: MG3b grassland soil bacteria 0-10cm


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NEWCASTLE UNIVERSITY, UK

text: public

text: elevated

characteristic: Submitter Id

characteristic: collection date

characteristic: depth

text: grassland soil

characteristic: environment (biome)

text: pasture

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: United Kingdom

text: metagenome

characteristic: investigation type

text: CACGCTACGT

characteristic: multiplex identifiers

text: soil metagenome

text: soil metagenome

text: pyrosequencing

text: soil

text: MG3b grassland soil bacteria 0-10cm

characteristic: title


text: NEWCASTLE UNIVERSITY, UK

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: control

characteristic: SRA accession

characteristic: adapters

text: grassland soil

text: pasture

text: soil

text: United Kingdom

characteristic: geographic location (country and/or sea)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

text: CAGTAGACGT

text: soil metagenome

characteristic: organism

characteristic: pcr primers

characteristic: project name

text: soil metagenome

text: pyrosequencing

text: soil

text: MG3b grassland soil bacteria 0-10cm


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NEWCASTLE UNIVERSITY, UK

text: public

text: control

characteristic: Submitter Id

characteristic: collection date

characteristic: depth

text: grassland soil

characteristic: environment (biome)

text: pasture

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: United Kingdom

text: metagenome

text: CGACGTGACT

text: soil metagenome

text: soil metagenome

text: pyrosequencing

characteristic: sequencing method

text: soil

characteristic: soil environmental package

characteristic: soil type

text: MG3b grassland soil bacteria 0-10cm


text: NEWCASTLE UNIVERSITY, UK

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: control

characteristic: SRA accession

characteristic: adapters

text: grassland soil

text: pasture

text: soil

text: United Kingdom

characteristic: geographic location (country and/or sea)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: TACACACACT

characteristic: multiplex identifiers

text: soil metagenome

text: soil metagenome

text: pyrosequencing

text: soil

text: MG3b grassland soil bacteria 0-10cm

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NEWCASTLE UNIVERSITY, UK

text: public

text: control

characteristic: Submitter Id

characteristic: collection date

characteristic: depth

text: grassland soil

characteristic: environment (biome)

text: pasture

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: United Kingdom

text: metagenome

text: TACACGTGAT

text: soil metagenome

characteristic: organism

characteristic: pcr primers

characteristic: project name

text: soil metagenome

text: pyrosequencing

text: soil

text: MG3b grassland soil bacteria 0-10cm


text: NEWCASTLE UNIVERSITY, UK

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: elevated

characteristic: SRA accession

characteristic: adapters

text: grassland soil

text: pasture

text: soil

text: United Kingdom

characteristic: geographic location (country and/or sea)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

text: TACAGATCGT

text: soil metagenome

text: soil metagenome

text: pyrosequencing

characteristic: sequencing method

text: soil

characteristic: soil environmental package

characteristic: soil type

text: MG3b grassland soil bacteria 0-10cm


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NEWCASTLE UNIVERSITY, UK

text: public

text: elevated

characteristic: Submitter Id

characteristic: collection date

characteristic: depth

text: grassland soil

characteristic: environment (biome)

text: pasture

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: United Kingdom

text: metagenome

characteristic: investigation type

text: TACGCTGTCT

characteristic: multiplex identifiers

text: soil metagenome

text: soil metagenome

text: pyrosequencing

text: soil

text: MG3b grassland soil bacteria 0-10cm

characteristic: title


text: NEWCASTLE UNIVERSITY, UK

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: elevated

characteristic: SRA accession

characteristic: adapters

text: grassland soil

text: pasture

text: soil

text: United Kingdom

characteristic: geographic location (country and/or sea)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

text: TAGTGTAGAT

text: soil metagenome

characteristic: organism

characteristic: pcr primers

characteristic: project name

text: soil metagenome

text: pyrosequencing

text: soil

text: MG3b grassland soil bacteria 0-10cm


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NEWCASTLE UNIVERSITY, UK

text: public

text: elevated

characteristic: Submitter Id

characteristic: collection date

characteristic: depth

text: grassland soil

characteristic: environment (biome)

text: pasture

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: United Kingdom

text: metagenome

text: TCGATCACGT

text: soil metagenome

text: soil metagenome

text: pyrosequencing

characteristic: sequencing method

text: soil

characteristic: soil environmental package

characteristic: soil type

text: MG3b grassland soil bacteria 0-10cm


text: NEWCASTLE UNIVERSITY, UK

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: blank

characteristic: SRA accession

characteristic: adapters

text: grassland soil

text: pasture

text: soil

text: United Kingdom

characteristic: geographic location (country and/or sea)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: TCGCACTAGT

characteristic: multiplex identifiers

text: soil metagenome

text: soil metagenome

text: pyrosequencing

text: soil

text: blank

text: MG3b grassland soil bacteria 0-10cm

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

tag: Namespace:ALFRED WEGENER INSTITUTE FOR POLAR AND MARINE RESE

characteristic: Submitter Id

text: f: adapter A + key (4bp): CGTATCGCCTCCCTCGCGCCATCAG; r: adapter B + key (4bp): CTATGCGCCTTGCCAGCCCGCTCAG

unit: L

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

characteristic: body site

characteristic: collection date

text: Pacific oyster

characteristic: common name

text: Experimental sample. Sample title code: [original temperature][experimental temperature]_[infection]_[time point]. For details see study description and 'perturbation' and 'detailed conditions' fields

characteristic: description

text: 5 days after transfer from flowthrough to stationary seawater aquarium (both 8°C) and injection with Vibrio sp. D29w strain

characteristic: detailed conditions

text: dead

characteristic: disease status

text: aquatic, marine

characteristic: environment (biome)

text: organic

characteristic: environment (feature)

text: organic material, bodily fluid

characteristic: environment (material)

text: Germany

unit: ºC

characteristic: host body temperature

text: Pacific oyster

characteristic: host common name

characteristic: host subject id

characteristic: host taxid

text: host-associated

characteristic: host-associated environmental package

text: mimarks-survey

text: adult

characteristic: life stage

text: Schleswig-Holstein, Wadden Sea (North Sea), island of Sylt, Kongishafen

characteristic: location details

text: f: CGTACAGTCA; r: ACGCTCGACA or f: CGTACAGTCA; r: AGCACTGTAG

text: Crassostrea gigas

characteristic: organism

text: initial denaturation:94degC_1min; denaturation:94degC_40s;annealing:55degC_30s; elongation:72degC_30s; final elongation:72degC_2min; 35 cycles

characteristic: pcr conditions

text: f: linker (3bp) + 16S_338r: CATGCTGCCTCCCGTAGGAGT; r: linker (2bp) + 16S_27F: TCAGAGTTTGATCMTGGCTCAG

characteristic: pcr primers

text: temperature acclimated, infected

characteristic: perturbation

characteristic: project name

text: syringe

characteristic: sample collection device or method

text: Crassostrea gigas

text: pyrosequencing

text: seawater (local, supplied through cca 200 m long pipe, 30 um filtered and protein skimmed)

characteristic: substrate

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: temperature

text: C.gigas hemolymph microbiota CC_1_5


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ALFRED WEGENER INSTITUTE FOR POLAR AND MARINE RESE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

characteristic: body site

characteristic: collection date

text: Pacific oyster

characteristic: common name

characteristic: description

text: 3 days after transfer from flowthrough to stationary seawater aquarium, temperature change from 8°C to 22°C and injection with Vibrio sp. D29w strain

characteristic: detailed conditions

text: dead

characteristic: disease status

text: aquatic, marine

characteristic: environment (biome)

text: organic

characteristic: environment (feature)

text: organic material, bodily fluid

characteristic: environment (material)

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host body temperature

text: Pacific oyster

text: host-associated

text: mimarks-survey

characteristic: investigation type

text: adult

text: f: TACACGTGAT; r: AGACGCACTC or f: TACACGTGAT; r: ATATCGCGAG

characteristic: multiplex identifiers

text: Crassostrea gigas

text: temperature stressed, infected

text: syringe

text: Crassostrea gigas

text: pyrosequencing

characteristic: sequencing method

text: 16S rRNA

text: C.gigas hemolymph microbiota CW_1_3

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

text: Pacific oyster

text: 3 days after transfer from flowthrough to stationary seawater aquarium (both 22°C) and injection with soya-peptone medium

text: healthy

text: aquatic, marine

text: organic

text: organic material, bodily fluid

text: Germany

text: Pacific oyster

characteristic: host common name

characteristic: host subject id

characteristic: host taxid

text: host-associated

characteristic: host-associated environmental package

text: mimarks-survey

text: adult

characteristic: life stage

characteristic: location details

text: f: TACACACACT; r: AGACGCACTC or f: TACACACACT; r: ATATCGCGAG

text: Crassostrea gigas

characteristic: organism

characteristic: pcr conditions

characteristic: pcr primers

text: temperature acclimated, mock-infected

characteristic: perturbation

characteristic: project name

text: syringe

characteristic: sample collection device or method

text: Crassostrea gigas

text: pyrosequencing

characteristic: substrate

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: temperature

text: C.gigas hemolymph microbiota WW_0_3


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

characteristic: body site

characteristic: collection date

text: Pacific oyster

characteristic: common name

characteristic: description

text: 5 days after transfer from flowthrough to stationary seawater aquarium (both 22°C) and injection with soya-peptone medium

characteristic: detailed conditions

text: healthy

characteristic: disease status

text: aquatic, marine

characteristic: environment (biome)

text: organic

characteristic: environment (feature)

text: organic material, bodily fluid

characteristic: environment (material)

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host body temperature

text: Pacific oyster

text: host-associated

text: mimarks-survey

characteristic: investigation type

text: adult

text: f: ACGACAGCTC; r: ACGCTCGACA or f: ACGACAGCTC; r: AGCACTGTAG

characteristic: multiplex identifiers

text: Crassostrea gigas

text: temperature acclimated, mock-infected

text: syringe

text: Crassostrea gigas

text: pyrosequencing

characteristic: sequencing method

text: 16S rRNA

text: C.gigas hemolymph microbiota WW_0_5

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

text: Pacific oyster

text: 5 days after transfer from flowthrough to stationary seawater aquarium (both 8°C) and injection with soya-peptone medium

text: healthy

text: aquatic, marine

text: organic

text: organic material, bodily fluid

text: Germany

text: Pacific oyster

characteristic: host common name

characteristic: host subject id

characteristic: host taxid

text: host-associated

characteristic: host-associated environmental package

text: mimarks-survey

text: adult

characteristic: life stage

characteristic: location details

text: f: TGAGTCAGTA; r: ACGCTCGACA or f: TGAGTCAGTA; r: AGCACTGTAG

text: Crassostrea gigas

characteristic: organism

characteristic: pcr conditions

characteristic: pcr primers

text: temperature acclimated, mock-infected

characteristic: perturbation

characteristic: project name

text: syringe

characteristic: sample collection device or method

text: Crassostrea gigas

text: pyrosequencing

characteristic: substrate

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: temperature

text: C.gigas hemolymph microbiota CC_0_5


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: f: adapter A + key (4bp): CGTATCGCCTCCCTCGCGCCATCAG; r: adapter B + key (4bp): CTATGCGCCTTGCCAGCCCGCTCAG

characteristic: adapters

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

characteristic: body site

characteristic: collection date

text: Pacific oyster

characteristic: common name

characteristic: description

text: 3 days after transfer from flowthrough to stationary seawater aquarium, temperature change from 8°C to 22°C and injection with soya-peptone medium

characteristic: detailed conditions

text: healthy

characteristic: disease status

text: aquatic, marine

characteristic: environment (biome)

text: organic

characteristic: environment (feature)

text: organic material, bodily fluid

characteristic: environment (material)

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host body temperature

text: Pacific oyster

text: host-associated

text: mimarks-survey

characteristic: investigation type

text: adult

text: f: TACGCTGTCT; r: AGACGCACTC or f: TACGCTGTCT; r: ATATCGCGAG

characteristic: multiplex identifiers

text: Crassostrea gigas

text: temperature stressed, mock-infected

text: syringe

text: Crassostrea gigas

text: pyrosequencing

characteristic: sequencing method

text: 16S rRNA

text: C.gigas hemolymph microbiota CW_0_3

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

text: Pacific oyster

text: Experimental sample. Sample title code: [original temperature][experimental temperature]_[infection]_[time point]. For details see study description and 'perturbation' and 'detailed conditions' fields

text: 3 days after transfer from flowthrough to stationary seawater aquarium, temperature change from 22°C to 8°C and injection with Vibrio sp. D29w strain

text: healthy

text: aquatic, marine

text: organic

text: organic material, bodily fluid

text: Germany

text: Pacific oyster

characteristic: host common name

characteristic: host subject id

characteristic: host taxid

text: host-associated

characteristic: host-associated environmental package

text: mimarks-survey

text: adult

characteristic: life stage

text: Schleswig-Holstein, Wadden Sea (North Sea), island of Sylt, Kongishafen

characteristic: location details

text: f: TACTCTCGTG; r: AGACGCACTC or f: TACTCTCGTG; r: ATATCGCGAG

text: Crassostrea gigas

characteristic: organism

characteristic: pcr conditions

characteristic: pcr primers

text: temperature stressed, infected

characteristic: perturbation

characteristic: project name

text: syringe

characteristic: sample collection device or method

text: Crassostrea gigas

text: pyrosequencing

characteristic: substrate

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: temperature

text: C.gigas hemolymph microbiota WC_1_3


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

characteristic: body site

characteristic: collection date

text: Pacific oyster

characteristic: common name

characteristic: description

text: held in flowthrough aquarium, 22°C, well acclimated

characteristic: detailed conditions

text: healthy

characteristic: disease status

text: aquatic, marine

characteristic: environment (biome)

text: organic

characteristic: environment (feature)

text: organic material, bodily fluid

characteristic: environment (material)

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host body temperature

text: Pacific oyster

text: host-associated

text: mimarks-survey

characteristic: investigation type

text: adult

text: f: AGACGCACTC; r: ACGCTCGACA or f: AGACGCACTC; r: AGCACTGTAG

characteristic: multiplex identifiers

text: Crassostrea gigas

text: initial denaturation:94degC_1min; denaturation:94degC_40s;annealing:55degC_30s; elongation:72degC_30s; final elongation:72degC_2min; 35 cycles

text: f: linker (3bp) + 16S_338r: CATGCTGCCTCCCGTAGGAGT; r: linker (2bp) + 16S_27F: TCAGAGTTTGATCMTGGCTCAG

text: temperature acclimated

text: syringe

text: Crassostrea gigas

text: pyrosequencing

characteristic: sequencing method

text: seawater (local, supplied through cca 200 m long pipe, 30 um filtered and protein skimmed)

text: 16S rRNA

text: C.gigas hemolymph microbiota WC_1_0

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ALFRED WEGENER INSTITUTE FOR POLAR AND MARINE RESE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

text: Pacific oyster

text: 7 days after transfer from flowthrough to stationary seawater aquarium (both 22°C) and injection with Vibrio sp. D29w strain

text: healthy

text: aquatic, marine

text: organic

text: organic material, bodily fluid

text: Germany

text: Pacific oyster

characteristic: host common name

characteristic: host subject id

characteristic: host taxid

text: host-associated

characteristic: host-associated environmental package

text: mimarks-survey

text: adult

characteristic: life stage

characteristic: location details

text: f: AGTAGTGATC; r: AGACGCACTC or f: AGTAGTGATC; r: ATATCGCGAG

text: Crassostrea gigas

characteristic: organism

characteristic: pcr conditions

characteristic: pcr primers

text: temperature acclimated, infected

characteristic: perturbation

characteristic: project name

text: syringe

characteristic: sample collection device or method

text: Crassostrea gigas

text: pyrosequencing

characteristic: substrate

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: temperature

text: C.gigas hemolymph microbiota WW_1_7


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

characteristic: body site

characteristic: collection date

text: Pacific oyster

characteristic: common name

characteristic: description

text: 3 days after transfer from flowthrough to stationary seawater aquarium (both 22°C) and injection with Vibrio sp. D29w strain

characteristic: detailed conditions

text: healthy

characteristic: disease status

text: aquatic, marine

characteristic: environment (biome)

text: organic

characteristic: environment (feature)

text: organic material, bodily fluid

characteristic: environment (material)

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host body temperature

text: Pacific oyster

text: host-associated

text: mimarks-survey

characteristic: investigation type

text: adult

text: f: TGACGTATGT; r: AGACGCACTC or f: TGACGTATGT; r: ATATCGCGAG

characteristic: multiplex identifiers

text: Crassostrea gigas

text: temperature acclimated, infected

text: syringe

text: Crassostrea gigas

text: pyrosequencing

characteristic: sequencing method

text: 16S rRNA

text: C.gigas hemolymph microbiota WW_1_3

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: f: adapter A + key (4bp): CGTATCGCCTCCCTCGCGCCATCAG; r: adapter B + key (4bp): CTATGCGCCTTGCCAGCCCGCTCAG

characteristic: adapters

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

text: Pacific oyster

text: 7 days after transfer from flowthrough to stationary seawater aquarium (both 8°C) and injection with Vibrio sp. D29w strain

text: healthy

text: aquatic, marine

text: organic

text: organic material, bodily fluid

text: Germany

text: Pacific oyster

characteristic: host common name

characteristic: host subject id

characteristic: host taxid

text: host-associated

characteristic: host-associated environmental package

text: mimarks-survey

text: adult

characteristic: life stage

characteristic: location details

text: f: TAGTCGCATA; r: AGACGCACTC or f: TAGTCGCATA; r: ATATCGCGAG

text: Crassostrea gigas

characteristic: organism

characteristic: pcr conditions

characteristic: pcr primers

text: temperature acclimated, infected

characteristic: perturbation

characteristic: project name

text: syringe

characteristic: sample collection device or method

text: Crassostrea gigas

text: pyrosequencing

characteristic: substrate

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: temperature

text: C.gigas hemolymph microbiota CC_1_7


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

characteristic: body site

characteristic: collection date

text: Pacific oyster

characteristic: common name

text: Experimental sample. Sample title code: [original temperature][experimental temperature]_[infection]_[time point]. For details see study description and 'perturbation' and 'detailed conditions' fields

characteristic: description

text: held in flowthrough aquarium, 8°C, well acclimated

characteristic: detailed conditions

text: diseased

characteristic: disease status

text: aquatic, marine

characteristic: environment (biome)

text: organic

characteristic: environment (feature)

text: organic material, bodily fluid

characteristic: environment (material)

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host body temperature

text: Pacific oyster

text: host-associated

text: mimarks-survey

characteristic: investigation type

text: adult

text: f: TCTATACTAT; r: ACGCTCGACA or f: TCTATACTAT; r: AGCACTGTAG

characteristic: multiplex identifiers

text: Crassostrea gigas

text: temperature acclimated

text: syringe

text: Crassostrea gigas

text: pyrosequencing

characteristic: sequencing method

text: 16S rRNA

text: C.gigas hemolymph microbiota CW_1_0

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

text: Pacific oyster

text: 5 days after transfer from flowthrough to stationary seawater aquarium, temperature change from 22°C to 8°C and injection with soya-peptone medium

text: healthy

text: aquatic, marine

text: organic

text: organic material, bodily fluid

text: Germany

text: Pacific oyster

characteristic: host common name

characteristic: host subject id

characteristic: host taxid

text: host-associated

characteristic: host-associated environmental package

text: mimarks-survey

text: adult

characteristic: life stage

text: Schleswig-Holstein, Wadden Sea (North Sea), island of Sylt, Kongishafen

characteristic: location details

text: f: TGTCGTCGCA; r: ACGCTCGACA or f: TGTCGTCGCA; r: AGCACTGTAG

text: Crassostrea gigas

characteristic: organism

characteristic: pcr conditions

characteristic: pcr primers

text: temperature stressed, mock-infected

characteristic: perturbation

characteristic: project name

text: syringe

characteristic: sample collection device or method

text: Crassostrea gigas

text: pyrosequencing

characteristic: substrate

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: temperature

text: C.gigas hemolymph microbiota WC_0_5


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

characteristic: body site

characteristic: collection date

text: Pacific oyster

characteristic: common name

characteristic: description

characteristic: detailed conditions

text: healthy

characteristic: disease status

text: aquatic, marine

characteristic: environment (biome)

text: organic

characteristic: environment (feature)

text: organic material, bodily fluid

characteristic: environment (material)

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host body temperature

text: Pacific oyster

text: host-associated

text: mimarks-survey

characteristic: investigation type

text: adult

text: f: CTACGCTCTA; r: ACGCTCGACA or f: CTACGCTCTA; r: AGCACTGTAG

characteristic: multiplex identifiers

text: Crassostrea gigas

text: initial denaturation:94degC_1min; denaturation:94degC_40s;annealing:55degC_30s; elongation:72degC_30s; final elongation:72degC_2min; 35 cycles

text: temperature stressed, mock-infected

text: syringe

text: Crassostrea gigas

text: pyrosequencing

characteristic: sequencing method

text: 16S rRNA

text: C.gigas hemolymph microbiota WC_0_5

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

text: Pacific oyster

text: 7 days after transfer from flowthrough to stationary seawater aquarium, temperature change from 22°C to 8°C and injection with soya-peptone medium

text: healthy

text: aquatic, marine

text: organic

text: organic material, bodily fluid

text: Germany

text: Pacific oyster

characteristic: host common name

characteristic: host subject id

characteristic: host taxid

text: host-associated

characteristic: host-associated environmental package

text: mimarks-survey

text: adult

characteristic: life stage

characteristic: location details

text: f: TGTCGTCGCA; r: AGACGCACTC or f: TGTCGTCGCA; r: ATATCGCGAG

text: Crassostrea gigas

characteristic: organism

characteristic: pcr conditions

text: f: linker (3bp) + 16S_338r: CATGCTGCCTCCCGTAGGAGT; r: linker (2bp) + 16S_27F: TCAGAGTTTGATCMTGGCTCAG

characteristic: pcr primers

text: temperature stressed, mock-infected

characteristic: perturbation

characteristic: project name

text: syringe

characteristic: sample collection device or method

text: Crassostrea gigas

text: pyrosequencing

text: seawater (local, supplied through cca 200 m long pipe, 30 um filtered and protein skimmed)

characteristic: substrate

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: temperature

text: C.gigas hemolymph microbiota WC_0_7


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ALFRED WEGENER INSTITUTE FOR POLAR AND MARINE RESE

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

characteristic: body site

characteristic: collection date

text: Pacific oyster

characteristic: common name

characteristic: description

characteristic: detailed conditions

text: healthy

characteristic: disease status

text: aquatic, marine

characteristic: environment (biome)

text: organic

characteristic: environment (feature)

text: organic material, bodily fluid

characteristic: environment (material)

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host body temperature

text: Pacific oyster

text: host-associated

text: mimarks-survey

characteristic: investigation type

text: adult

text: f: CTCGCGTGTC; r: ACGCTCGACA or f: CTCGCGTGTC; r: AGCACTGTAG

characteristic: multiplex identifiers

text: Crassostrea gigas

text: temperature acclimated

text: syringe

text: Crassostrea gigas

text: pyrosequencing

characteristic: sequencing method

text: 16S rRNA

text: C.gigas hemolymph microbiota CC_1_0

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: f: adapter A + key (4bp): CGTATCGCCTCCCTCGCGCCATCAG; r: adapter B + key (4bp): CTATGCGCCTTGCCAGCCCGCTCAG

characteristic: adapters

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

text: Pacific oyster

text: 5 days after transfer from flowthrough to stationary seawater aquarium, temperature change from 22°C to 8°C and injection with Vibrio sp. D29w strain

text: healthy

text: aquatic, marine

text: organic

text: organic material, bodily fluid

text: Germany

text: Pacific oyster

characteristic: host common name

characteristic: host subject id

characteristic: host taxid

text: host-associated

characteristic: host-associated environmental package

text: mimarks-survey

text: adult

characteristic: life stage

characteristic: location details

text: f: AGCTCACGTA; r: ACGCTCGACA or f: AGCTCACGTA; r: AGCACTGTAG

text: Crassostrea gigas

characteristic: organism

characteristic: pcr conditions

characteristic: pcr primers

text: temperature stressed, infected

characteristic: perturbation

characteristic: project name

text: syringe

characteristic: sample collection device or method

text: Crassostrea gigas

text: pyrosequencing

characteristic: substrate

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: temperature

text: C.gigas hemolymph microbiota WC_1_5


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

characteristic: body site

characteristic: collection date

text: Pacific oyster

characteristic: common name

text: Experimental sample. Sample title code: [original temperature][experimental temperature]_[infection]_[time point]. For details see study description and 'perturbation' and 'detailed conditions' fields

characteristic: description

text: 3 days after transfer from flowthrough to stationary seawater aquarium, temperature change from 22°C to 8°C and injection with Vibrio sp. D29w strain

characteristic: detailed conditions

text: healthy

characteristic: disease status

text: aquatic, marine

characteristic: environment (biome)

text: organic

characteristic: environment (feature)

text: organic material, bodily fluid

characteristic: environment (material)

text: Germany

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

characteristic: host body temperature

text: Pacific oyster

text: host-associated

text: mimarks-survey

characteristic: investigation type

text: adult

text: f: ACGAGTGCGT; r: AGACGCACTC or f: ACGAGTGCGT; r: ATATCGCGAG

characteristic: multiplex identifiers

text: Crassostrea gigas

text: temperature stressed, infected

text: syringe

text: Crassostrea gigas

text: pyrosequencing

characteristic: sequencing method

text: 16S rRNA

text: C.gigas hemolymph microbiota WC_1_3

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: adapters

characteristic: amount or size of sample collected

text: hemolymph

characteristic: body habitat

text: hemolymph

characteristic: body product

text: adductor muscle

text: Pacific oyster

text: 7 days after transfer from flowthrough to stationary seawater aquarium (both 8°C) and injection with Vibrio sp. D29w strain

text: dead

text: aquatic, marine

text: organic

text: organic material, bodily fluid

text: Germany

text: Pacific oyster

characteristic: host common name

characteristic: host subject id

characteristic: host taxid

text: host-associated

characteristic: host-associated environmental package

text: mimarks-survey

text: adult

characteristic: life stage

characteristic: location details

text: f: TGTCACACGA; r: AGACGCACTC or f: TGTCACACGA; r: ATATCGCGAG

text: Crassostrea gigas

characteristic: organism

characteristic: pcr conditions

characteristic: pcr primers

text: temperature acclimated, infected

characteristic: perturbation

characteristic: project name

text: syringe

characteristic: sample collection device or method

text: Crassostrea gigas

text: pyrosequencing

characteristic: substrate

text: 16S rRNA

characteristic: target gene

characteristic: target subfragment

characteristic: temperature

text: C.gigas hemolymph microbiota CC_1_7