characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EAWAG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: 36 samples

text: wastewater

text: wastewater treatment plant

text: activated sludge

text: Switzerland

text: metagenome

text: metagenome

characteristic: organism

text: metagenome

text: Illumina HiSeq

text: Unprocessed DNA and RNA sequences from wastewater treatment plant communities

characteristic: title

text: wastewater/sludge


status: PUBLIC

tag: attribute

text: EAWAG

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: 36 samples

characteristic: description

text: wastewater

characteristic: environment (biome)

text: wastewater treatment plant

characteristic: environment (feature)

text: activated sludge

characteristic: environment (material)

text: Switzerland

characteristic: geographic location (country and/or sea)

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: metagenome

text: What biological and operational factors determine the release of antibiotic resistance genes from WWTPs?

characteristic: project name

text: metagenome

text: Illumina HiSeq

characteristic: sequencing method

text: Unprocessed DNA and RNA from wastewater treatment plant communities

text: wastewater/sludge

characteristic: wastewater/sludge environmental package


E-MTAB-4620:gcn5 delete_replicate #1

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-4620:gcn5 delete_replicate #1

tag: Namespace:Univ Lyon, ENS de Lyon, Univ Claude Bernard, CNRS UMR 5239, INSERM U1210

text: ArrayExpress

characteristic: broker name

text: Protocols: Since condensin accumulates on chromosomes during mitosis, fission yeast cells were collected during prometaphase prior to chromatin digestion by MNase. Cells bearing the cryosensitive mutation nda3-km311 and expressing the cnd2-GFP subunit of condensin were exponentially grown at 32°c and shifted to 19°C for 7 hours to induce a cell-cycle arrest in pro/metaphase by the nda3-km311. Mitotic indexes were measured by scoring the nuclear accumulation of cnd2-GFP. Cells were fixed with 0.5% formaldehyde for 30 minutes, washed with H2O and stored at -80°C. Cells were spheroplasted and MNase digestion of mitotic chromatin was performed as described (Lantermann et al, 2009) using cells arrested in early mitosis by the nda3-km311 mutation at 19°C and crosslinked with 0.5% formaldehyde for 30 min 2x109. Chromatin was digested with increasing amount of MNase to reach a mononucleosome to di-nucleosome ratio of ~80:20. Mononucleosomal DNA fragments were separated on an agarose gel, extracted from the gel and subjected to massive parallel sequencing on an Illumina NextSeq 500 Apparatus. Ref: Lantermann A, et al. (2009) Genome-wide mapping of nucleosome positions in Schizosaccharomyces pombe. Methods San Diego Calif 48: 218-225 Libraries were prepared according to instructions accompanying the NEB-Next Kit for Illumina. Briefly, gel-purified mononucleosomal DNA fragments (146 bp-long) were end-repaired. Blunt ends were treated with Klenow fragment and dATP to produce protruding 3’ A' for ligating Illumina's adapters. Resulting DNA was PCR amplified 4 cycles. Libraries were sequenced on Illumina NextSeq 500 sequencer following the manufacturer's protocols.

text: gcn5 deletion

characteristic: genotype

text: Schizosaccharomyces pombe 972h-

characteristic: organism

text: Schizosaccharomyces pombe 972h-

text: replicate 1

characteristic: replicate

text: Schizosaccharomyces pombe 972h-

text: gcn5 delete_replicate #1

type: has member


E-MTAB-4620:gcn5 delete_replicate #2

text: Univ Lyon, ENS de Lyon, Univ Claude Bernard, CNRS UMR 5239, INSERM U1210

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-4620:gcn5 delete_replicate #2

characteristic: Submitter Id

text: ArrayExpress

characteristic: description

text: gcn5 deletion

text: Schizosaccharomyces pombe 972h-

text: Schizosaccharomyces pombe 972h-

text: replicate 2

text: Schizosaccharomyces pombe 972h-

text: gcn5 delete_replicate #2

characteristic: title


E-MTAB-4620:gcn5 delete_replicate #3

text: public

text: E-MTAB-4620:gcn5 delete_replicate #3

text: ArrayExpress

text: gcn5 deletion

text: Schizosaccharomyces pombe 972h-

text: Schizosaccharomyces pombe 972h-

text: replicate 3

text: Schizosaccharomyces pombe 972h-

text: gcn5 delete_replicate #3


name: E-MTAB-4620:gcn5 mst2 double-delete_replicate #1

text: public

text: ArrayExpress

text: gcn5 deletion and mst2 deletion

text: Schizosaccharomyces pombe 972h-

text: Schizosaccharomyces pombe 972h-

text: replicate 1

text: Schizosaccharomyces pombe 972h-

text: gcn5 mst2 double-delete_replicate #1


name: E-MTAB-4620:gcn5 mst2 double-delete_replicate #2

text: public

text: ArrayExpress

text: gcn5 deletion and mst2 deletion

text: Schizosaccharomyces pombe 972h-

text: Schizosaccharomyces pombe 972h-

text: replicate 2

text: Schizosaccharomyces pombe 972h-

text: gcn5 mst2 double-delete_replicate #2


name: E-MTAB-4620:gcn5 mst2 double-delete_replicate #3

text: public

text: ArrayExpress

text: gcn5 deletion and mst2 deletion

text: Schizosaccharomyces pombe 972h-

text: Schizosaccharomyces pombe 972h-

text: replicate 3

text: Schizosaccharomyces pombe 972h-

text: gcn5 mst2 double-delete_replicate #3


E-MTAB-4620:wt replicate #1

text: public

text: E-MTAB-4620:wt replicate #1

text: ArrayExpress

text: wild type genotype

text: Schizosaccharomyces pombe 972h-

text: Schizosaccharomyces pombe 972h-

text: replicate 1

text: Schizosaccharomyces pombe 972h-

text: wt replicate #1


E-MTAB-4620:wt replicate #2

text: public

text: E-MTAB-4620:wt replicate #2

text: ArrayExpress

text: wild type genotype

text: Schizosaccharomyces pombe 972h-

text: Schizosaccharomyces pombe 972h-

text: replicate 2

text: Schizosaccharomyces pombe 972h-

text: wt replicate #2


E-MTAB-4620:wt replicate #3

text: public

text: E-MTAB-4620:wt replicate #3

text: ArrayExpress

text: wild type genotype

text: Schizosaccharomyces pombe 972h-

text: Schizosaccharomyces pombe 972h-

text: replicate 3

text: Schizosaccharomyces pombe 972h-

text: wt replicate #3


text: CEA Saclay

text: public

tag: Namespace:CEA Saclay

text: ArrayExpress

text: baker's yeast

characteristic: common name

text: Protocols: All strains were grown in YPD rich medium at 30°C to 0.6 OD600, then shifted at 37°C for 90min The cell pellets are resuspended in 1 ml of FA/SDS/PMSF buffer (50 mM HEPES-KOH, pH 7.5, 150 mM NaCl, 1 mM EDTA, 1% Triton X100, 0.1% sodium deoxycholate, 0.1% SDS, 1 mM PMSF) and transferred to 1.5 mL Eppendorf tubes. 0.75 mL glass beads (425-600 μm, Sigma) are then added to each tube, and the yeast cells were lysed at 4°C for 15 min by vortexing. Pierce the bottom of a 12 ml Greiner tube with a hot needle (0.7x30 mm or 0.5x15 mm) and place in a Falcon50 with a pierced cap. The lysates are transfered in the pierced tubes. Wash each Epp with twice 1 ml of FA/SDS/PMSF buffer (4 ml per extract). Spin down 1 min/2000 rpm. Gently resuspend the pellet with a 5 ml pipet and transfer in a sterile 15 ml Corex tube. Spin down 20 min / 12000 rpm / 4°C in a JA17 rotor (Beckmann). Vacuum the supernatant. The cross-linked chromatin appears as a transparent layer around the pellet. Transfer the pellet in a 2 ml Epp with a burned Pasteur pipet. Add 0.8 ml of FA/SDS/PMSF buffer in the Corex and resuspend with the Pasteur pipet. Transfer in the Epp. Wash the Corex with 0.8 ml FA/SDS/PMSF buffer. Homogenize the Epp with the Pasteur pipet and incubate on a rotating wheal at 4°C for 1 to 2 hours.Spin down 20 min / 12000 rpm / 4°C. Vacuum the supernatant and resuspend in 1.6 ml FA/SDS/PMSF buffer with a burned Pasteur pipet.Place the tubes in a mix water / ice / NaCl. Sonicator settings: Pulse - 60% - Power 4. Sonicate 3 times 40 sec with 20 sec intervals. Split in 800μl aliquots and performe an additional sonication step with Bioruptor (Diagenode), 6 cycles of 40s with medium intensity setting. Average size of fragments obtained is 200bp.Transfer in a 12 ml Greiner tube and add 0.5 ml FA/SDS/PMSF buffer. Incubate on a rotating wheal for 30 min to 1 hour at 4°C. Spin down 30 min / 10000 rpm / 4°C on a JA20 rotor (Beckmann).Transfer the supernatant in a new tube. Aliquot the supernatant (the chromatin is now soluble). Aliquots of 650 μl and freeze in liquid nitrogen.Chromatin fragment control: Take an aliquot of 100 μl of chromatin and add 25 μl Pronase buffer 5X and 6.25 μl of Pronase (20 mg/ml in H20). Incubate 1 hour at 37 °C and O/N at 65 °C (digestproteins and reverse crosslink). Add 3.5 μl of RNaseA 1mg/ml and incubate 1 hour at 37 °C. Purify on a Quiagen PCR purification column (Elute wih 50 μl Tris 10 mM pH8.5). Load on gel (1.5% in TAE -100V).Immunoprecipitation:Wash 50 μl of Dynabeads Ig 4 times with 500 μl PBS BSA 0.1% buffer. Resuspend in 100 μl PBS BSA 0.1 % buffer. Add antibody (5μl 8WG16 or 1μL 12CA5 or any for TAp tagged strains). Incubate 30 min at 1300 rpm in an Eppendorf shaker at 30 °C. Wash 2X 500 μl PBS BSA 0.1%. Wash 1X 500 μl PBS BSA 0.1% with shaking 10 min at 1300 rpm / 30 °C. Wash 1X 500 μl PBS BSA 0.1%.Spin down the chromatin from the -80°C for 15 min / 12000 rpm / 4 °C. Add 50 μl of PBS BSA 10mg/ml to the beads and then 500 μl of chromatin.Incubate 2h at 21 °C in an Eppendorf shaker (1300 rpm). Resuspend with 500 μl FA/SDS and change tube. Wash 2X 1 ml FA/SDS + NaCl (final concentration 500 mM). Wash 1X 1 ml FA/SDS + NaCl with shaking 10 min at 1300 rpm / 21 °C. Wash 1X with 500 μl IP buffer (Tris pH7.5 125 mM, EDTA 25 mM, SDS 2.5%). Wash 1X with 500 μl TE. Elute with 125 μl Pronase Buffer 1X for 20 min at 65 °C in an Eppendorf shaker at 600 rpm. Cool down at RT. Add 6.25 μl Pronase to the supernatant. Incubate 1h at 37 °C and O/N at 65 °C. Add 25 μl of Pronase buffer 5X and 6.25 μl of Pronase to 100 μl of chromatin remaining from the initial tube (total DNA).Incubate 1h at 37 °C and O/N at 65 °C. Add 3.5 μl RNAseA (1mg/ml) and incubate 1h at 37 °C. Purify DNA on a Qiagen PCR purification column. Illumina TruSeq ChIP Library Prep Kit

text: tagging

characteristic: genetic modification

text: MATa ura3-52 his3-D200 ade2-101uaa trp1-D63 lys2-801uag leu2-D1 RAD3::3HA::HIS3 med10::KanMX6 // med10-196 CEN TRP1

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: CEA Saclay

text: public

text: ArrayExpress

text: baker's yeast

text: tagging

text: MATa ura3-52 his3-D200 ade2-101uaa trp1-D63 lys2-801uag leu2-D1 RAD3::3HA::HIS3 med10::KanMX6 // MED10 CEN TRP1

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: CEA Saclay

text: public

text: ArrayExpress

text: baker's yeast

text: tagging

text: MATa ura3-52 his3-D200 ade2-101uaa trp1-D63 lys2-801uag leu2-D1 KIN28::3HA::HIS3 med10::KanMX6 // med10-196 CEN TRP1

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: CEA Saclay

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ArrayExpress

text: baker's yeast

text: tagging

text: MATa ura3-52 his3-D200 ade2-101uaa trp1-D63 lys2-801uag leu2-D1 KIN28::3HA::HIS3 med10::KanMX6 // MED10 CEN TRP1

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: CEA Saclay

characteristic: INSDC last update

text: public

text: ArrayExpress

characteristic: broker name

text: baker's yeast

text: tagging

text: MATa ura3-52 his3-D200 ade2-101uaa trp1-D63 lys2-801uag leu2-D1 MED15::3HA::HIS3 med10::KanMX6 // med10-196 CEN TRP1

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: CEA Saclay

text: public

text: ArrayExpress

text: baker's yeast

text: tagging

text: MATa ura3-52 his3-D200 ade2-101uaa trp1-D63 lys2-801uag leu2-D1 MED15::3HA::HIS3 med10::KanMX6 // MED10 CEN TRP1

characteristic: genotype

text: Saccharomyces cerevisiae

characteristic: organism

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: CEA Saclay

text: public

characteristic: INSDC status

text: ArrayExpress

text: baker's yeast

text: tagging

text: MATa ura3-52 his3-D200 ade2-101uaa trp1-D63 lys2-801uag leu2-D1 MED17::3HA::HIS3 med10::KanMX6 // med10-196 CEN TRP1

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: CEA Saclay

text: public

characteristic: SRA accession

text: ArrayExpress

text: baker's yeast

text: tagging

text: MATa ura3-52 his3-D200 ade2-101uaa trp1-D63 lys2-801uag leu2-D1 MED17::3HA::HIS3 med10::KanMX6 // MED10 CEN TRP1

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: CEA Saclay

text: public

characteristic: Submitter Id

text: ArrayExpress

text: baker's yeast

text: wild type

text: MATa ura3-52 his3-D200 ade2-101uaa trp1-D63 lys2-801uag leu2-D1 med10::KanMX6 // MED10 CEN TRP1

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: CEA Saclay

text: public

text: ArrayExpress

text: baker's yeast

characteristic: description

text: tagging

text: MATa ura3-52 his3-D200 ade2-101uaa trp1-D63 lys2-801uag leu2-D1 LEU2::3HA::SPT15 med10::KanMX6 // med10-196 CEN TRP1

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: CEA Saclay

text: public

text: ArrayExpress

text: baker's yeast

text: tagging

text: MATa ura3-52 his3-D200 ade2-101uaa trp1-D63 lys2-801uag leu2-D1 LEU2::3HA::SPT15 med10::KanMX6 // MED10 CEN TRP1

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

characteristic: title


text: CEA Saclay

text: public

text: ArrayExpress

text: baker's yeast

characteristic: common name

text: tagging

text: MATa ura3-52 his3-D200 ade2-101uaa trp1-D63 lys2-801uag leu2-D1 TFA2::TAP::HIS3 med10::KanMX6 // med10-196 CEN TRP1

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: CEA Saclay

text: public

text: ArrayExpress

text: baker's yeast

text: tagging

characteristic: genetic modification

text: MATa ura3-52 his3-D200 ade2-101uaa trp1-D63 lys2-801uag leu2-D1 TFA2::TAP::HIS3 med10::KanMX6 // MED10 CEN TRP1

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


characteristic: ENA-CHECKLIST

text: CEA Saclay

text: public

text: ArrayExpress

text: baker's yeast

text: tagging

text: MATa ura3-52 his3-D200 ade2-101uaa trp1-D63 lys2-801uag leu2-D1 TFG1::TAP::HIS3 med10::KanMX6 // med10-196 CEN TRP1

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


characteristic: ENA-FIRST-PUBLIC

text: CEA Saclay

text: public

text: ArrayExpress

text: baker's yeast

text: tagging

text: MATa ura3-52 his3-D200 ade2-101uaa trp1-D63 lys2-801uag leu2-D1 TFG1::TAP::HIS3 med10::KanMX6 // MED10 CEN TRP1

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


characteristic: ENA-LAST-UPDATE

text: CEA Saclay

text: public

text: ArrayExpress

text: baker's yeast

text: tagging

text: MATa ura3-52 his3-D200 ade2-101uaa trp1-D63 lys2-801uag leu2-D1 SUA7::TAP::HIS3 med10::KanMX6 // med10-196 CEN TRP1

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


characteristic: External Id

text: CEA Saclay

text: public

text: ArrayExpress

text: baker's yeast

text: Protocols: All strains were grown in YPD rich medium at 30°C to 0.6 OD600, then shifted at 37°C for 90min The cell pellets are resuspended in 1 ml of FA/SDS/PMSF buffer (50 mM HEPES-KOH, pH 7.5, 150 mM NaCl, 1 mM EDTA, 1% Triton X100, 0.1% sodium deoxycholate, 0.1% SDS, 1 mM PMSF) and transferred to 1.5 mL Eppendorf tubes. 0.75 mL glass beads (425-600 μm, Sigma) are then added to each tube, and the yeast cells were lysed at 4°C for 15 min by vortexing. Pierce the bottom of a 12 ml Greiner tube with a hot needle (0.7x30 mm or 0.5x15 mm) and place in a Falcon50 with a pierced cap. The lysates are transfered in the pierced tubes. Wash each Epp with twice 1 ml of FA/SDS/PMSF buffer (4 ml per extract). Spin down 1 min/2000 rpm. Gently resuspend the pellet with a 5 ml pipet and transfer in a sterile 15 ml Corex tube. Spin down 20 min / 12000 rpm / 4°C in a JA17 rotor (Beckmann). Vacuum the supernatant. The cross-linked chromatin appears as a transparent layer around the pellet. Transfer the pellet in a 2 ml Epp with a burned Pasteur pipet. Add 0.8 ml of FA/SDS/PMSF buffer in the Corex and resuspend with the Pasteur pipet. Transfer in the Epp. Wash the Corex with 0.8 ml FA/SDS/PMSF buffer. Homogenize the Epp with the Pasteur pipet and incubate on a rotating wheal at 4°C for 1 to 2 hours.Spin down 20 min / 12000 rpm / 4°C. Vacuum the supernatant and resuspend in 1.6 ml FA/SDS/PMSF buffer with a burned Pasteur pipet.Place the tubes in a mix water / ice / NaCl. Sonicator settings: Pulse - 60% - Power 4. Sonicate 3 times 40 sec with 20 sec intervals. Split in 800μl aliquots and performe an additional sonication step with Bioruptor (Diagenode), 6 cycles of 40s with medium intensity setting. Average size of fragments obtained is 200bp.Transfer in a 12 ml Greiner tube and add 0.5 ml FA/SDS/PMSF buffer. Incubate on a rotating wheal for 30 min to 1 hour at 4°C. Spin down 30 min / 10000 rpm / 4°C on a JA20 rotor (Beckmann).Transfer the supernatant in a new tube. Aliquot the supernatant (the chromatin is now soluble). Aliquots of 650 μl and freeze in liquid nitrogen.Chromatin fragment control: Take an aliquot of 100 μl of chromatin and add 25 μl Pronase buffer 5X and 6.25 μl of Pronase (20 mg/ml in H20). Incubate 1 hour at 37 °C and O/N at 65 °C (digestproteins and reverse crosslink). Add 3.5 μl of RNaseA 1mg/ml and incubate 1 hour at 37 °C. Purify on a Quiagen PCR purification column (Elute wih 50 μl Tris 10 mM pH8.5). Load on gel (1.5% in TAE -100V).Immunoprecipitation:Wash 50 μl of Dynabeads Ig 4 times with 500 μl PBS BSA 0.1% buffer. Resuspend in 100 μl PBS BSA 0.1 % buffer. Add antibody (5μl 8WG16 or 1μL 12CA5 or any for TAp tagged strains). Incubate 30 min at 1300 rpm in an Eppendorf shaker at 30 °C. Wash 2X 500 μl PBS BSA 0.1%. Wash 1X 500 μl PBS BSA 0.1% with shaking 10 min at 1300 rpm / 30 °C. Wash 1X 500 μl PBS BSA 0.1%.Spin down the chromatin from the -80°C for 15 min / 12000 rpm / 4 °C. Add 50 μl of PBS BSA 10mg/ml to the beads and then 500 μl of chromatin.Incubate 2h at 21 °C in an Eppendorf shaker (1300 rpm). Resuspend with 500 μl FA/SDS and change tube. Wash 2X 1 ml FA/SDS + NaCl (final concentration 500 mM). Wash 1X 1 ml FA/SDS + NaCl with shaking 10 min at 1300 rpm / 21 °C. Wash 1X with 500 μl IP buffer (Tris pH7.5 125 mM, EDTA 25 mM, SDS 2.5%). Wash 1X with 500 μl TE. Elute with 125 μl Pronase Buffer 1X for 20 min at 65 °C in an Eppendorf shaker at 600 rpm. Cool down at RT. Add 6.25 μl Pronase to the supernatant. Incubate 1h at 37 °C and O/N at 65 °C. Add 25 μl of Pronase buffer 5X and 6.25 μl of Pronase to 100 μl of chromatin remaining from the initial tube (total DNA).Incubate 1h at 37 °C and O/N at 65 °C. Add 3.5 μl RNAseA (1mg/ml) and incubate 1h at 37 °C. Purify DNA on a Qiagen PCR purification column. Illumina TruSeq ChIP Library Prep Kit

text: tagging

text: MATa ura3-52 his3-D200 ade2-101uaa trp1-D63 lys2-801uag leu2-D1 SUA7::TAP::HIS3 med10::KanMX6 // MED10 CEN TRP1

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: CEA Saclay

characteristic: INSDC center name

text: public

text: ArrayExpress

text: baker's yeast

text: tagging

text: MATa ura3-52 his3-D200 ade2-101uaa trp1-D63 lys2-801uag leu2-D1 TOA2::TAP::HIS3 med10::KanMX6 // med10-196 CEN TRP1

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: CEA Saclay

characteristic: INSDC first public

text: public

text: ArrayExpress

text: baker's yeast

text: tagging

text: MATa ura3-52 his3-D200 ade2-101uaa trp1-D63 lys2-801uag leu2-D1 TOA2::TAP::HIS3 med10::KanMX6 // MED10 CEN TRP1

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: FZB

characteristic: INSDC last update

text: public

text: MTBC complex

text: Mycobacterium tuberculosis complex bacterium

text: WGS of MTBC isolates


text: FRIEDRICH SCHILLER UNIVERSITY JENA

text: public

tag: Namespace:FRIEDRICH SCHILLER UNIVERSITY JENA

characteristic: culture collection

text: minus

characteristic: mating type

text: Absidia glauca

text: Absidia glauca

text: CBS 101.48

characteristic: strain

text: RVII-324 met-

characteristic: sub strain


text: testis

characteristic: ArrayExpress-OrganismPart

text: male

characteristic: ArrayExpress-Sex

text: Mus musculus

characteristic: ArrayExpress-Species

text: SC

text: public

characteristic: INSDC status

characteristic: SRA accession

text: house mouse

text: Mus musculus

characteristic: organism

text: TruSeq Small RNA library

characteristic: sample description

text: Mus musculus


text: Corynebacterium minutissimum

characteristic: ArrayExpress-StrainOrLine

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

characteristic: Submitter Id

text: Corynebacterium minutissimum

characteristic: common name

text: Corynebacterium minutissimum

text: Corynebacterium minutissimum

characteristic: title


text: Pseudomonas synxantha

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: Pseudomonas synxantha

text: Pseudomonas synxantha

text: Pseudomonas synxantha


text: Burkholderia cenocepacia

text: SC

text: public

text: Burkholderia cenocepacia

text: Burkholderia cenocepacia

text: Burkholderia cenocepacia

characteristic: strain


text: FRIEDRICH SCHILLER UNIVERSITY JENA

text: public

text: CBS 101.48

characteristic: culture collection

characteristic: mating type

text: Absidia glauca

text: Absidia glauca

text: RVII-324 met-


text: ELTE

text: public

characteristic: INSDC status

characteristic: SRA accession

text: chicken

text: Wild type DT40 starting clone

characteristic: description

text: Gallus gallus

unit: bp

characteristic: read length

text: Gallus gallus


characteristic: ENA-CHECKLIST

text: ELTE

text: public

text: chicken

text: Wild type DT40 clone with strong mutagenic treatment

text: Gallus gallus

characteristic: organism

text: Gallus gallus


characteristic: ENA-FIRST-PUBLIC

text: ELTE

text: public

characteristic: Submitter Id

text: chicken

text: Wild type DT40 clone with weak mutagenic treatment

text: Gallus gallus

text: Gallus gallus


text: ELTE

text: public

text: chicken

characteristic: common name

text: Wild type DT40 clone 1 of an identical sample pair

text: Gallus gallus

text: Gallus gallus

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ELTE

text: public

text: chicken

text: Wild type DT40 clone 2 of an identical sample pair

text: Gallus gallus

text: Gallus gallus


text: ELTE

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: chicken

text: Mutant 1 DT40 starting clone

text: Gallus gallus

text: Gallus gallus


text: ELTE

characteristic: INSDC last update

text: public

text: chicken

text: Mutant 1 DT40 clone with strong mutagenic treatment

text: Gallus gallus

text: Gallus gallus


text: ELTE

text: public

text: chicken

text: Mutant 1 DT40 clone with weak mutagenic treatment

text: Gallus gallus

text: Gallus gallus


text: ELTE

text: public

text: chicken

text: Mutant 1 DT40 clone 1 of an identical sample pair

text: Gallus gallus

text: Gallus gallus


text: ELTE

text: public

text: chicken

text: Mutant 1 DT40 clone 2 of an identical sample pair

text: Gallus gallus

text: Gallus gallus


text: NORTH CAROLINA STATE UNIVERSITY

text: public

tag: Namespace:NORTH CAROLINA STATE UNIVERSITY

text: Stephanie Mathews

characteristic: collected by

characteristic: collection date

text: Black Liquor

characteristic: isolation source

text: Paenibacillus glucanolyticus

text: Paenibacillus glucanolyticus

text: Paenibacillus glucanolyticus SLM1


text: NORTH CAROLINA STATE UNIVERSITY

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Alexander and Priest

text: soil

text: Paenibacillus glucanolyticus

text: Paenibacillus glucanolyticus

text: Paenibacillus glucanolyticus 5162


text: FRIEDRICH SCHILLER UNIVERSITY JENA

text: public

characteristic: culture collection

text: minus

characteristic: mating type

text: Absidia glauca

text: Absidia glauca

text: CBS 101.48

characteristic: strain

text: RVII-324 met-

characteristic: sub strain

text: Assembly


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: SVERIGES LANTBRUKSUNIVERSITET

text: public

tag: Namespace:SVERIGES LANTBRUKSUNIVERSITET

characteristic: Submitter Id

text: Setaria digitata

characteristic: organism

text: Setaria digitata

text: DNA extracted from Setaria digital in Sri Lanka


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SVERIGES LANTBRUKSUNIVERSITET

text: public

text: Setaria digitata

text: Setaria digitata

text: Draft assembly of Setaria digital

characteristic: title


Sample 211

text: INSTITUTE OF EVOLUTIONARY BIOLOGY

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: Sample 211

tag: Namespace:INSTITUTE OF EVOLUTIONARY BIOLOGY

text: Replicate 211

characteristic: description

text: Biorhiza pallida

text: Biorhiza pallida

text: Replicate raw reads


text: Staphylococcus aureus

characteristic: ArrayExpress-Species

characteristic: ArrayExpress-StrainOrLine

text: SC

text: public

characteristic: INSDC status

text: Staphylococcus aureus

characteristic: common name

text: Staphylococcus aureus

text: Staphylococcus aureus


text: CGG

text: public

characteristic: SRA accession

characteristic: collection date

text: common ragweed

text: 33.841883, -93.77575

characteristic: lat lon

text: Ambrosia artemisiifolia

text: Ambrosia artemisiifolia

text: leaf

characteristic: tissue type


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: CGG

text: public

characteristic: Submitter Id

text: common ragweed

text: -27.148033, 152.569135

text: Ambrosia artemisiifolia

text: Ambrosia artemisiifolia

text: leaf


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: CGG

text: public

text: common ragweed

text: -28.100028, 153.116677

text: Ambrosia artemisiifolia

characteristic: organism

text: Ambrosia artemisiifolia

text: leaf


text: CGG

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: common ragweed

text: -28.416469, 153.200021

text: Ambrosia artemisiifolia

text: Ambrosia artemisiifolia

text: leaf

characteristic: title


text: CGG

characteristic: INSDC last update

text: public

text: common ragweed

characteristic: description

text: 46.472052, 6.336436

text: Ambrosia artemisiifolia

text: Ambrosia artemisiifolia

text: leaf


text: CGG

text: public

characteristic: INSDC status

text: common ragweed

text: 25.541789, -80.412844

text: Ambrosia artemisiifolia

text: Ambrosia artemisiifolia

text: leaf


text: CGG

text: public

text: common ragweed

characteristic: common name

text: 27.374365, -80.416042

text: Ambrosia artemisiifolia

text: Ambrosia artemisiifolia

text: leaf


text: CGG

text: public

characteristic: SRA accession

text: common ragweed

text: 27.085217, -81.794967

text: Ambrosia artemisiifolia

text: Ambrosia artemisiifolia

text: leaf


text: CGG

text: public

characteristic: collection date

text: common ragweed

text: 31.678667, -83.196133

text: Ambrosia artemisiifolia

text: Ambrosia artemisiifolia

text: leaf


text: CGG

text: public

text: common ragweed

text: Budapest, Hungary

characteristic: geographic location (region and locality)

text: Ambrosia artemisiifolia

text: Ambrosia artemisiifolia

text: leaf


text: CGG

text: public

text: common ragweed

text: Cegled, Hungary

text: Ambrosia artemisiifolia

text: Ambrosia artemisiifolia

text: leaf

characteristic: tissue type


characteristic: ENA-CHECKLIST

text: CGG

text: public

text: common ragweed

text: Nyiregyhaza, Hungary

text: Ambrosia artemisiifolia

text: Ambrosia artemisiifolia

text: leaf


characteristic: ENA-FIRST-PUBLIC

text: CGG

text: public

text: common ragweed

text: 41.874983, -93.482783

characteristic: lat lon

text: Ambrosia artemisiifolia

text: Ambrosia artemisiifolia

text: leaf


characteristic: ENA-LAST-UPDATE

text: CGG

text: public

characteristic: Submitter Id

text: common ragweed

text: 39.23775, -88.151217

text: Ambrosia artemisiifolia

text: Ambrosia artemisiifolia

text: leaf


characteristic: External Id

text: CGG

text: public

text: common ragweed

text: 30.468783, -90.6429

text: Ambrosia artemisiifolia

characteristic: organism

text: Ambrosia artemisiifolia

text: leaf


text: CGG

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: common ragweed

text: 44.632296, -69.507862

text: Ambrosia artemisiifolia

text: Ambrosia artemisiifolia

text: leaf


text: CGG

characteristic: INSDC last update

text: public

text: common ragweed

text: 44.013321, -70.069435

text: Ambrosia artemisiifolia

text: Ambrosia artemisiifolia

text: leaf

characteristic: title


text: CGG

text: public

characteristic: INSDC status

text: common ragweed

characteristic: description

text: 45.157841, -67.289035

text: Ambrosia artemisiifolia

text: Ambrosia artemisiifolia

text: leaf


text: CGG

text: public

characteristic: SRA accession

text: common ragweed

characteristic: common name

text: 46.172383, -84.36515

text: Ambrosia artemisiifolia

text: Ambrosia artemisiifolia

text: leaf


text: CGG

text: public

characteristic: collection date

text: common ragweed

text: 43.15405, -84.503539

text: Ambrosia artemisiifolia

text: Ambrosia artemisiifolia

text: leaf


text: CGG

text: public

text: common ragweed

text: 45.793567, -94.24465

text: Ambrosia artemisiifolia

text: Ambrosia artemisiifolia

text: leaf

characteristic: tissue type


characteristic: ENA-CHECKLIST

text: CGG

text: public

text: common ragweed

text: 38.968433, -93.879417

text: Ambrosia artemisiifolia

text: Ambrosia artemisiifolia

text: leaf


characteristic: ENA-FIRST-PUBLIC

text: CGG

text: public

text: common ragweed

text: 38.386347, -90.25932

text: Ambrosia artemisiifolia

text: Ambrosia artemisiifolia

text: leaf


text: CGG

text: public

text: common ragweed

text: 39.709343, -91.500199

characteristic: lat lon

text: Ambrosia artemisiifolia

text: Ambrosia artemisiifolia

text: leaf


characteristic: ENA-LAST-UPDATE

text: CGG

text: public

text: common ragweed

text: 42.890685, -72.527665

text: Ambrosia artemisiifolia

text: Ambrosia artemisiifolia

text: leaf


text: CGG

text: public

characteristic: Submitter Id

text: common ragweed

text: 40.740027, -74.953111

text: Ambrosia artemisiifolia

text: Ambrosia artemisiifolia

text: leaf


characteristic: External Id

text: CGG

text: public

text: common ragweed

text: 39.149171, -74.851037

text: Ambrosia artemisiifolia

text: Ambrosia artemisiifolia

text: leaf


text: CGG

text: public

text: common ragweed

text: 40.226333, -74.257833

text: Ambrosia artemisiifolia

characteristic: organism

text: Ambrosia artemisiifolia

text: leaf


text: CGG

characteristic: INSDC center name

text: public

text: common ragweed

text: 41.62921, -74.182352

text: Ambrosia artemisiifolia

text: Ambrosia artemisiifolia

text: leaf


text: CGG

characteristic: INSDC first public

text: public

text: common ragweed

text: 44.908797, -65.177264

text: Ambrosia artemisiifolia

text: Ambrosia artemisiifolia

text: leaf


text: CGG

characteristic: INSDC last update

text: public

text: common ragweed

text: 44.754723, -63.671654

text: Ambrosia artemisiifolia

text: Ambrosia artemisiifolia

text: leaf


text: CGG

text: public

text: common ragweed

text: 38.909567, -82.728417

text: Ambrosia artemisiifolia

text: Ambrosia artemisiifolia

text: leaf

characteristic: title


text: CGG

text: public

characteristic: INSDC status

text: common ragweed

text: 40.0785, -79.61485

text: Ambrosia artemisiifolia

text: Ambrosia artemisiifolia

text: leaf


text: CGG

text: public

text: common ragweed

characteristic: description

text: 41.683117, -71.134283

text: Ambrosia artemisiifolia

text: Ambrosia artemisiifolia

text: leaf


text: CGG

text: public

characteristic: SRA accession

text: common ragweed

text: 34.792517, -80.415333

text: Ambrosia artemisiifolia

text: Ambrosia artemisiifolia

text: leaf


text: CGG

text: public

text: common ragweed

characteristic: common name

text: 36.119433, -85.0589

text: Ambrosia artemisiifolia

text: Ambrosia artemisiifolia

text: leaf


text: CGG

text: public

characteristic: collection date

text: common ragweed

text: 45.415167, -89.190017

text: Ambrosia artemisiifolia

text: Ambrosia artemisiifolia

text: leaf


text: CGG

text: public

text: common ragweed

text: Ambrosia artemisiifolia

text: Ambrosia artemisiifolia

text: leaf

characteristic: tissue type


characteristic: ENA-CHECKLIST

text: INSTITUTO NACIONAL DE SALUD

text: public

tag: Namespace:INSTITUTO NACIONAL DE SALUD

text: ESBL-producing and hypermucoviscous Klebsiella quasipneumoniae subsp. similipneumoniae clinical isolate

text: Mexico

characteristic: geographic location (country and/or sea)

text: Human

characteristic: host

text: Urine

characteristic: isolation source


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: UCSDMI

text: public

characteristic: Submitter Id

characteristic: age

characteristic: age group

text: years

characteristic: age unit

characteristic: altitude

characteristic: anonymized name

characteristic: antibiotics

text: False

text: GTAGATCGTGTA

characteristic: barcodesequence

text: UBERON:feces

characteristic: body habitat

text: UBERON:feces

characteristic: body product

text: UBERON:feces

characteristic: body site

text: Chihuahua

characteristic: breed

text: Toy/Companion

text: gut metagenome

text: GAZ:Germany

characteristic: country

characteristic: depth

text: False

characteristic: dewormed

text: acute hem. diarrhea

text: True

characteristic: elevation

characteristic: emp status

text: ENVO:urban biome

characteristic: env biome

text: ENVO:animal-associated habitat

characteristic: env feature

text: ENVO:feces

characteristic: env matter

characteristic: experiment title

text: Texas A&M

text: Fecal samples from dogs of various breeds, places of origin, and severity of bowel disorder were sequencing to obtain a dog gut metagenome.

text: F

text: True

text: True

text: domestic dog

characteristic: host common name

text: Canis lupus familiaris

characteristic: host scientific name

characteristic: host subject id

characteristic: host taxid

text: HiSeq

characteristic: latitude

text: This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.

characteristic: library construction protocol

text: GT

characteristic: linker

text: GTGCCAGCMGCCGCGGTAA

characteristic: linkerprimersequence

characteristic: longitude

text: gut metagenome

text: FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT

characteristic: pcr primers

text: CCME

text: Texas A&M

text: False

text: Illumina

characteristic: platform

characteristic: platformchemistry

characteristic: pool name

text: False

text: completed

text: CCME

text: 0.1, gram

text: Texas A&M

text: gut metagenome

text: sequencing by synthesis

text: female

text: small

text: Texas A&M

text: 16S rRNA

text: False


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

characteristic: age group

text: years

characteristic: age unit

characteristic: altitude

characteristic: anonymized name

characteristic: antibiotics

text: False

text: TAACGTGTGTGC

characteristic: barcodesequence

text: UBERON:feces

characteristic: body habitat

text: UBERON:feces

characteristic: body product

text: UBERON:feces

characteristic: body site

characteristic: breed

text: Working

characteristic: collection date

text: gut metagenome

characteristic: common name

text: GAZ:Germany

characteristic: country

characteristic: depth

characteristic: description

text: acute hem. diarrhea

text: True

characteristic: elevation

characteristic: emp status

text: ENVO:urban biome

characteristic: env biome

text: ENVO:animal-associated habitat

text: ENVO:feces

text: Texas A&M

text: MC

text: True

text: True

text: domestic dog

text: Canis lupus familiaris

text: HiSeq

text: GT

text: GTGCCAGCMGCCGCGGTAA

text: gut metagenome

characteristic: organism

text: CCME

text: Texas A&M

text: False

text: Illumina

text: False

text: completed

text: CCME

text: 0.1, gram

text: Texas A&M

text: gut metagenome

text: sequencing by synthesis

text: male

text: medium

text: Texas A&M

text: 16S rRNA

text: False


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

characteristic: age group

text: years

characteristic: age unit

characteristic: altitude

text: False

text: CATTATGGCGTG

text: UBERON:feces

text: UBERON:feces

text: UBERON:feces

text: Mixed

text: gut metagenome

text: GAZ:Germany

text: True

characteristic: dewormed

text: acute hem. diarrhea

text: True

text: ENVO:urban biome

text: ENVO:animal-associated habitat

characteristic: env feature

text: ENVO:feces

characteristic: env matter

characteristic: experiment title

text: Texas A&M

text: FS

text: True

text: True

text: domestic dog

characteristic: host common name

text: Canis lupus familiaris

characteristic: host scientific name

characteristic: host subject id

characteristic: host taxid

text: HiSeq

characteristic: latitude

characteristic: library construction protocol

text: GT

characteristic: linker

text: GTGCCAGCMGCCGCGGTAA

characteristic: linkerprimersequence

characteristic: longitude

text: gut metagenome

characteristic: pcr primers

text: CCME

text: Texas A&M

text: False

text: Illumina

characteristic: platform

characteristic: platformchemistry

characteristic: pool name

text: False

text: completed

text: CCME

text: 0.1, gram

text: Texas A&M

text: gut metagenome

text: sequencing by synthesis

text: female

text: Texas A&M

text: 16S rRNA

text: False


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

characteristic: age group

text: years

characteristic: age unit

characteristic: altitude

characteristic: anonymized name

characteristic: antibiotics

text: False

text: CCAATACGCCTG

characteristic: barcodesequence

text: UBERON:feces

characteristic: body habitat

text: UBERON:feces

characteristic: body product

text: UBERON:feces

characteristic: body site

characteristic: breed

text: Working

characteristic: collection date

text: gut metagenome

characteristic: common name

text: GAZ:Germany

characteristic: country

characteristic: depth

characteristic: description

text: acute hem. diarrhea

text: True

characteristic: elevation

characteristic: emp status

text: ENVO:urban biome

characteristic: env biome

text: ENVO:animal-associated habitat

text: ENVO:feces

text: Texas A&M

text: FS

text: True

text: True

text: domestic dog

text: Canis lupus familiaris

text: HiSeq

text: GT

text: GTGCCAGCMGCCGCGGTAA

text: gut metagenome

characteristic: organism

text: CCME

text: Texas A&M

text: False

text: Illumina

text: False

text: completed

text: CCME

text: 0.1, gram

text: Texas A&M

text: gut metagenome

text: sequencing by synthesis

text: female

text: medium

text: Texas A&M

text: 16S rRNA

text: True


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

characteristic: age group

text: years

characteristic: age unit

characteristic: altitude

text: False

text: GATCTGCGATCC

text: UBERON:feces

text: UBERON:feces

text: UBERON:feces

text: Working

text: gut metagenome

text: GAZ:Germany

text: False

characteristic: dewormed

text: acute hem. diarrhea

text: True

text: ENVO:urban biome

text: ENVO:animal-associated habitat

characteristic: env feature

text: ENVO:feces

characteristic: env matter

characteristic: experiment title

text: Texas A&M

text: M

text: True

text: True

text: domestic dog

characteristic: host common name

text: Canis lupus familiaris

characteristic: host scientific name

characteristic: host subject id

characteristic: host taxid

text: HiSeq

characteristic: latitude

characteristic: library construction protocol

text: GT

characteristic: linker

text: GTGCCAGCMGCCGCGGTAA

characteristic: linkerprimersequence

characteristic: longitude

text: gut metagenome

characteristic: pcr primers

text: CCME

text: Texas A&M

text: False

text: Illumina

characteristic: platform

characteristic: platformchemistry

characteristic: pool name

text: False

text: completed

text: CCME

text: 0.1, gram

text: Texas A&M

text: gut metagenome

text: sequencing by synthesis

text: male

text: large

text: Texas A&M

text: 16S rRNA

text: True


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

characteristic: age group

text: years

characteristic: age unit

characteristic: altitude

characteristic: anonymized name

characteristic: antibiotics

text: False

text: CAGCTCATCAGC

characteristic: barcodesequence

text: UBERON:feces

characteristic: body habitat

text: UBERON:feces

characteristic: body product

text: UBERON:feces

characteristic: body site

characteristic: breed

text: Terrier

characteristic: collection date

text: gut metagenome

characteristic: common name

text: GAZ:Germany

characteristic: country

characteristic: depth

characteristic: description

text: False

characteristic: dewormed

text: acute hem. diarrhea

text: True

characteristic: elevation

characteristic: emp status

text: ENVO:urban biome

characteristic: env biome

text: ENVO:animal-associated habitat

text: ENVO:feces

text: Texas A&M

text: F

text: True

text: True

text: domestic dog

text: Canis lupus familiaris

text: HiSeq

text: GT

text: GTGCCAGCMGCCGCGGTAA

text: gut metagenome

characteristic: organism

text: CCME

text: Texas A&M

text: False

text: Illumina

text: False

text: completed

text: CCME

text: 0.1, gram

text: Texas A&M

text: gut metagenome

text: sequencing by synthesis

text: female

text: small

text: Texas A&M

text: 16S rRNA

text: True


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

characteristic: age group

text: years

characteristic: age unit

characteristic: altitude

characteristic: anonymized name

text: False

text: CAAACAACAGCT

text: UBERON:feces

text: UBERON:feces

text: UBERON:feces

text: Sporting

text: gut metagenome

text: GAZ:Germany

text: False

text: acute hem. diarrhea

text: True

text: ENVO:urban biome

text: ENVO:animal-associated habitat

characteristic: env feature

text: ENVO:feces

characteristic: env matter

characteristic: experiment title

text: Texas A&M

text: MC

text: True

text: True

text: domestic dog

characteristic: host common name

text: Canis lupus familiaris

characteristic: host scientific name

characteristic: host subject id

characteristic: host taxid

text: HiSeq

characteristic: latitude

characteristic: library construction protocol

text: GT

characteristic: linker

text: GTGCCAGCMGCCGCGGTAA

characteristic: linkerprimersequence

characteristic: longitude

text: gut metagenome

characteristic: pcr primers

text: CCME

text: Texas A&M

text: False

text: Illumina

characteristic: platform

characteristic: platformchemistry

characteristic: pool name

text: False

text: completed

text: CCME

text: 0.1, gram

text: Texas A&M

text: gut metagenome

text: sequencing by synthesis

text: male

text: large

text: Texas A&M

text: 16S rRNA

text: False


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

characteristic: age group

text: years

characteristic: age unit

characteristic: altitude

characteristic: anonymized name

characteristic: antibiotics

text: False

text: GCAACACCATCC

characteristic: barcodesequence

text: UBERON:feces

characteristic: body habitat

text: UBERON:feces

characteristic: body product

text: UBERON:feces

characteristic: body site

characteristic: breed

text: Terrier

characteristic: collection date

text: gut metagenome

characteristic: common name

text: GAZ:Germany

characteristic: country

characteristic: depth

characteristic: description

text: False

characteristic: dewormed

text: acute hem. diarrhea

text: True

characteristic: elevation

characteristic: emp status

text: ENVO:urban biome

characteristic: env biome

text: ENVO:animal-associated habitat

text: ENVO:feces

text: Texas A&M

text: FS

text: True

text: True

text: domestic dog

text: Canis lupus familiaris

text: HiSeq

text: GT

text: GTGCCAGCMGCCGCGGTAA

text: gut metagenome

characteristic: organism

text: CCME

text: Texas A&M

text: False

text: Illumina

text: False

text: completed

text: CCME

text: 0.1, gram

text: Texas A&M

text: gut metagenome

text: sequencing by synthesis

text: female

text: small

text: Texas A&M

text: 16S rRNA

text: True


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

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text: public

characteristic: INSDC status

characteristic: SRA accession

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characteristic: age

characteristic: age group

text: years

characteristic: age unit

characteristic: altitude

characteristic: anonymized name

characteristic: antibiotics

text: False

text: ATCCTTTGGTTC

characteristic: barcodesequence

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characteristic: body habitat

text: UBERON:feces

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text: UBERON:feces

characteristic: body site

text: Dachshund

characteristic: breed

text: Hound

characteristic: collection date

text: gut metagenome

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text: GAZ:Germany

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characteristic: dewormed

text: healthy

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characteristic: elevation

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text: ENVO:urban biome

characteristic: env biome

text: ENVO:animal-associated habitat

text: ENVO:feces

text: Texas A&M

text: M

text: True

text: True

text: domestic dog

text: Canis lupus familiaris

text: HiSeq

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text: GTGCCAGCMGCCGCGGTAA

text: gut metagenome

characteristic: organism

text: CCME

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text: Illumina

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text: 0.1, gram

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characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

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text: public

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characteristic: SRA accession

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characteristic: age

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text: years

characteristic: age unit

characteristic: altitude

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text: False

text: TACAGCGCATAC

text: UBERON:feces

text: UBERON:feces

text: UBERON:feces

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characteristic: env feature

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text: Texas A&M

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text: True

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text: domestic dog

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characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

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text: public

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characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

characteristic: age group

text: years

characteristic: age unit

characteristic: altitude

characteristic: anonymized name

characteristic: antibiotics

text: False

text: ACCGGTATGTAC

characteristic: barcodesequence

text: UBERON:feces

characteristic: body habitat

text: UBERON:feces

characteristic: body product

text: UBERON:feces

characteristic: body site

characteristic: breed

text: Mixed

characteristic: collection date

text: gut metagenome

characteristic: common name

text: GAZ:Germany

characteristic: country

characteristic: depth

characteristic: description

text: True

characteristic: dewormed

text: healthy

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text: ENVO:urban biome

characteristic: env biome

text: ENVO:animal-associated habitat

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text: domestic dog

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text: GTGCCAGCMGCCGCGGTAA

text: gut metagenome

characteristic: organism

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text: Illumina

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text: False


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

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text: public

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characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

characteristic: age group

text: years

characteristic: age unit

characteristic: altitude

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text: False

text: AATTGTGTCGGA

text: UBERON:feces

text: UBERON:feces

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text: Husky

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text: True

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text: ENVO:urban biome

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characteristic: env feature

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text: Texas A&M

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text: True

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characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

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text: public

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characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

characteristic: age group

text: years

characteristic: age unit

characteristic: altitude

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characteristic: antibiotics

text: False

text: TGCATACACTGG

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text: UBERON:feces

characteristic: body product

text: UBERON:feces

characteristic: body site

characteristic: breed

text: Mixed

characteristic: collection date

text: gut metagenome

characteristic: common name

text: GAZ:Germany

characteristic: country

characteristic: depth

characteristic: description

text: True

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text: healthy

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text: ENVO:urban biome

characteristic: env biome

text: ENVO:animal-associated habitat

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text: True

text: domestic dog

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text: gut metagenome

characteristic: organism

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text: female

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text: True


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

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text: public

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characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

characteristic: age group

text: years

characteristic: age unit

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characteristic: anonymized name

text: False

text: AGTCGAACGAGG

text: UBERON:feces

text: UBERON:feces

text: UBERON:feces

text: Terrier

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text: GAZ:Germany

text: True

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text: ENVO:urban biome

text: ENVO:animal-associated habitat

characteristic: env feature

text: ENVO:feces

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text: True

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text: domestic dog

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text: 0.1, gram

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text: False


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

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text: public

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characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

characteristic: age group

text: years

characteristic: age unit

characteristic: altitude

characteristic: anonymized name

characteristic: antibiotics

text: False

text: ACCAGTGACTCA

characteristic: barcodesequence

text: UBERON:feces

characteristic: body habitat

text: UBERON:feces

characteristic: body product

text: UBERON:feces

characteristic: body site

characteristic: breed

text: Sporting

characteristic: collection date

text: gut metagenome

characteristic: common name

text: GAZ:Germany

characteristic: country

characteristic: depth

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text: True

characteristic: dewormed

text: healthy

text: True

characteristic: elevation

characteristic: emp status

text: ENVO:urban biome

characteristic: env biome

text: ENVO:animal-associated habitat

text: ENVO:feces

text: Texas A&M

text: FS

text: True

text: True

text: domestic dog

text: Canis lupus familiaris

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text: GTGCCAGCMGCCGCGGTAA

text: gut metagenome

characteristic: organism

text: CCME

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text: False

text: Illumina

text: False

text: completed

text: CCME

text: 0.1, gram

text: Texas A&M

text: gut metagenome

text: sequencing by synthesis

text: female

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text: 16S rRNA

text: True


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

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text: public

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characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

characteristic: age group

text: years

characteristic: age unit

characteristic: altitude

characteristic: anonymized name

text: False

text: GAATACCAAGTC

text: UBERON:feces

text: UBERON:feces

text: UBERON:feces

text: Beagle

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text: gut metagenome

text: GAZ:Germany

text: True

text: healthy

text: True

text: ENVO:urban biome

text: ENVO:animal-associated habitat

characteristic: env feature

text: ENVO:feces

characteristic: env matter

characteristic: experiment title

text: Texas A&M

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text: True

text: True

text: domestic dog

characteristic: host common name

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text: False

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text: 0.1, gram

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text: 16S rRNA

text: False


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

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text: public

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characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

characteristic: age group

text: years

characteristic: age unit

characteristic: altitude

characteristic: anonymized name

characteristic: antibiotics

text: False

text: AGCTGTCAAGCT

characteristic: barcodesequence

characteristic: bcs

text: UBERON:feces

characteristic: body habitat

text: UBERON:feces

characteristic: body product

text: UBERON:feces

characteristic: body site

text: Rottweiler

characteristic: breed

text: Working

characteristic: collection date

text: gut metagenome

characteristic: common name

text: GAZ:United States of America

characteristic: country

characteristic: depth

characteristic: description

text: False

characteristic: dewormed

text: healthy

text: True

characteristic: elevation

characteristic: emp status

text: ENVO:urban biome

characteristic: env biome

text: ENVO:animal-associated habitat

text: ENVO:feces

text: Texas A&M

text: FS

text: True

text: True

text: domestic dog

text: Canis lupus familiaris

text: HiSeq

text: outdoor

text: GT

text: GTGCCAGCMGCCGCGGTAA

text: gut metagenome

characteristic: organism

text: CCME

text: Texas A&M

text: False

text: Illumina

text: FOS 1.0%

text: chicken

text: False

text: completed

text: CCME

text: 0.1, gram

text: Texas A&M

text: gut metagenome

text: sequencing by synthesis

text: female

text: large

text: California

text: California

text: Texas A&M

text: 16S rRNA

text: False


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

characteristic: age group

text: years

characteristic: age unit

characteristic: altitude

characteristic: anonymized name

characteristic: antibiotics

text: False

text: CGTGCTTAGGCT

text: UBERON:feces

text: UBERON:feces

text: UBERON:feces

text: Maltese

text: Toy/Companion

text: gut metagenome

text: GAZ:United States of America

text: True

text: healthy

text: True

text: ENVO:urban biome

text: ENVO:animal-associated habitat

characteristic: env feature

text: ENVO:feces

characteristic: env matter

characteristic: experiment title

text: Texas A&M

text: MC

text: True

text: True

text: domestic dog

characteristic: host common name

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characteristic: host scientific name

characteristic: host subject id

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text: indoor

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text: GTGCCAGCMGCCGCGGTAA

characteristic: linkerprimersequence

characteristic: longitude

text: gut metagenome

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text: Illumina

text: hydrolyzed

text: False

text: completed

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text: 0.1, gram

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text: gut metagenome

text: sequencing by synthesis

text: male

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text: California

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text: Texas A&M

text: 16S rRNA

text: True


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

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text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

characteristic: age group

text: years

characteristic: age unit

text: False

text: TACCGAAGGTAT

characteristic: barcodesequence

characteristic: bcs

text: UBERON:feces

characteristic: body habitat

text: UBERON:feces

characteristic: body product

text: UBERON:feces

characteristic: body site

text: Pug

characteristic: breed

text: Toy/Companion

characteristic: collection date

text: gut metagenome

characteristic: common name

text: GAZ:United States of America

characteristic: country

characteristic: depth

characteristic: description

text: healthy

text: True

characteristic: elevation

characteristic: emp status

text: ENVO:urban biome

characteristic: env biome

text: ENVO:animal-associated habitat

text: ENVO:feces

text: Texas A&M

text: FS

text: True

text: True

text: domestic dog

text: Canis lupus familiaris

text: HiSeq

text: indoor

text: GT

text: GTGCCAGCMGCCGCGGTAA

text: gut metagenome

characteristic: organism

text: CCME

text: Texas A&M

text: False

text: Illumina

text: chicken

text: False

text: completed

text: CCME

text: 0.1, gram

text: Texas A&M

text: gut metagenome

text: sequencing by synthesis

text: female

text: small

text: California

text: California

text: Texas A&M

text: 16S rRNA

text: True


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

characteristic: age group

text: years

characteristic: altitude

characteristic: anonymized name

characteristic: antibiotics

text: False

text: CACTCATCATTC

text: UBERON:feces

text: UBERON:feces

text: UBERON:feces

text: Sporting

text: gut metagenome

text: GAZ:United States of America

text: True

characteristic: dewormed

text: healthy

text: True

text: ENVO:urban biome

text: ENVO:animal-associated habitat

characteristic: env feature

text: ENVO:feces

characteristic: env matter

characteristic: experiment title

text: Texas A&M

text: M

text: True

text: True

text: domestic dog

characteristic: host common name

text: Canis lupus familiaris

characteristic: host scientific name

characteristic: host subject id

characteristic: host taxid

text: HiSeq

text: indoor

characteristic: latitude

characteristic: library construction protocol

text: GT

characteristic: linker

text: GTGCCAGCMGCCGCGGTAA

characteristic: linkerprimersequence

characteristic: longitude

text: gut metagenome

characteristic: pcr primers

text: CCME

text: Texas A&M

text: False

text: Illumina

text: chicken

text: False

text: completed

text: CCME

text: 0.1, gram

text: Texas A&M

text: gut metagenome

text: sequencing by synthesis

text: male

text: medium

text: California

text: California

text: Texas A&M

text: 16S rRNA

text: True


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

characteristic: age group

text: years

characteristic: age unit

characteristic: altitude

text: False

text: GTATTTCGGACG

characteristic: barcodesequence

characteristic: bcs

text: UBERON:feces

characteristic: body habitat

text: UBERON:feces

characteristic: body product

text: UBERON:feces

characteristic: body site

characteristic: breed

text: Toy/Companion

characteristic: collection date

text: gut metagenome

characteristic: common name

text: GAZ:United States of America

characteristic: country

characteristic: depth

characteristic: description

text: True

text: healthy

text: True

characteristic: elevation

characteristic: emp status

text: ENVO:urban biome

characteristic: env biome

text: ENVO:animal-associated habitat

text: ENVO:feces

text: Texas A&M

text: MC

text: True

text: True

text: domestic dog

text: Canis lupus familiaris

text: HiSeq

text: indoor

text: GT

text: GTGCCAGCMGCCGCGGTAA

text: gut metagenome

characteristic: organism

text: CCME

text: Texas A&M

text: False

text: Illumina

characteristic: platform

text: False

text: completed

text: CCME

text: 0.1, gram

text: Texas A&M

text: gut metagenome

text: sequencing by synthesis

text: male

text: small

text: California

text: California

text: Texas A&M

text: 16S rRNA

text: True


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

characteristic: age group

text: years

characteristic: age unit

characteristic: altitude

characteristic: anonymized name

characteristic: antibiotics

text: False

text: TATCTATCCTGC

text: UBERON:feces

text: UBERON:feces

text: UBERON:feces

text: Mixed

text: gut metagenome

text: GAZ:United States of America

text: False

characteristic: dewormed

text: healthy

text: True

text: ENVO:urban biome

text: ENVO:animal-associated habitat

characteristic: env feature

text: ENVO:feces

characteristic: env matter

characteristic: experiment title

text: Texas A&M

text: FS

text: True

text: True

text: domestic dog

characteristic: host common name

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characteristic: host scientific name

characteristic: host subject id

characteristic: host taxid

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characteristic: linkerprimersequence

characteristic: longitude

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text: Illumina

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text: 0.1, gram

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text: gut metagenome

text: sequencing by synthesis

text: female

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text: 16S rRNA

text: False


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

characteristic: age group

text: years

characteristic: age unit

characteristic: altitude

text: False

text: TTGCCAAGAGTC

characteristic: barcodesequence

characteristic: bcs

text: UBERON:feces

characteristic: body habitat

text: UBERON:feces

characteristic: body product

text: UBERON:feces

characteristic: body site

text: Chihuahua

characteristic: breed

text: Toy/Companion

characteristic: collection date

text: gut metagenome

characteristic: common name

text: GAZ:United States of America

characteristic: country

characteristic: depth

characteristic: description

text: healthy

text: True

characteristic: elevation

characteristic: emp status

text: ENVO:urban biome

characteristic: env biome

text: ENVO:animal-associated habitat

text: ENVO:feces

text: Texas A&M

text: MC

text: True

text: True

text: domestic dog

text: Canis lupus familiaris

text: HiSeq

text: indoor

text: GT

text: GTGCCAGCMGCCGCGGTAA

text: gut metagenome

characteristic: organism

text: CCME

text: Texas A&M

text: False

text: Illumina

text: chicken

text: False

text: completed

text: CCME

text: 0.1, gram

text: Texas A&M

text: gut metagenome

text: sequencing by synthesis

text: male

text: small

text: California

text: California

text: Texas A&M

text: 16S rRNA

text: False