Oilseed rape microbiome1

text: Institute of Technology Carlow

text: public

text: Oilseed rape microbiome1

tag: Namespace:Institute of Technology Carlow

characteristic: collection date

text: Effect of different tillage practices on microbiome associated to oilseed rape under Irish agronomic conditions. 16S rDNA amplicon based metagenomic analysis of oilseed rape microbiome during different developmental stages of oilseed rape plant.

text: Plant microbiome

characteristic: environment (biome)

text: Soil

characteristic: environment (feature)

text: Plant

characteristic: environment (material)

text: Ireland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: host-associated

characteristic: host-associated environmental package

text: metagenome

characteristic: investigation type

text: phyllosphere metagenome

text: Oilseed rape microbiome

text: phyllosphere metagenome

text: 16S rDNA amplicon Illumina MiSeq

characteristic: sequencing method

text: Metagenomic analysis of oilseed rape microbiome under Irish agronomic conditions


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UWASH

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

unit: m

characteristic: depth

text: T1 (Day 0) Control

characteristic: description

text: River delta

text: Sediment

text: Sediment

text: Greenland

characteristic: geographic location (elevation)

unit: DD

text: Southwest Greenland, Watson River

characteristic: geographic location (region and locality)

text: metagenome

text: unidentified prokaryotic organism

characteristic: organism

text: 341F, 806R

characteristic: pcr primers

text: Watson river delta sediment incubations

characteristic: project name

unit: g

characteristic: sample volume or weight for DNA extraction

text: unidentified prokaryotic organism

text: sediment

characteristic: sediment environmental package

text: MiSeq

text: T1 (Day 0) Control

characteristic: title

type: has member


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UWASH

text: public

characteristic: collection date

text: T1 (Day 0) Control autoclaved

text: River delta

characteristic: environment (biome)

text: Sediment

characteristic: environment (feature)

text: Sediment

characteristic: environment (material)

text: Greenland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Southwest Greenland, Watson River

text: metagenome

characteristic: investigation type

text: unidentified prokaryotic organism

text: 341F, 806R

text: Watson river delta sediment incubations

text: unidentified prokaryotic organism

text: sediment

text: MiSeq

characteristic: sequencing method

text: T1 (Day 0) Control autoclaved


text: UWASH

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: depth

text: T1 (Day 0) Methanol

characteristic: description

text: River delta

text: Sediment

text: Sediment

text: Greenland

characteristic: geographic location (elevation)

text: Southwest Greenland, Watson River

characteristic: geographic location (region and locality)

text: metagenome

text: unidentified prokaryotic organism

characteristic: organism

text: 341F, 806R

characteristic: pcr primers

text: Watson river delta sediment incubations

characteristic: project name

text: unidentified prokaryotic organism

text: sediment

text: MiSeq

text: T1 (Day 0) Methanol


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UWASH

text: public

characteristic: collection date

text: T1 (Day 0) Methanol autoclaved

text: River delta

characteristic: environment (biome)

text: Sediment

characteristic: environment (feature)

text: Sediment

characteristic: environment (material)

text: Greenland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: Southwest Greenland, Watson River

text: metagenome

text: unidentified prokaryotic organism

text: 341F, 806R

text: Watson river delta sediment incubations

characteristic: sample volume or weight for DNA extraction

text: unidentified prokaryotic organism

text: sediment

characteristic: sediment environmental package

text: MiSeq

text: T1 (Day 0) Methanol autoclaved

characteristic: title


text: UWASH

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: depth

text: T1 (Day 0) Mix-gas

characteristic: description

text: River delta

text: Sediment

text: Sediment

text: Greenland

characteristic: geographic location (elevation)

characteristic: geographic location (longitude)

text: Southwest Greenland, Watson River

characteristic: geographic location (region and locality)

text: metagenome

characteristic: investigation type

text: unidentified prokaryotic organism

text: 341F, 806R

text: Watson river delta sediment incubations

text: unidentified prokaryotic organism

text: sediment

text: MiSeq

characteristic: sequencing method

text: T1 (Day 0) Mix-gas


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UWASH

text: public

characteristic: collection date

text: T1 (Day 0) Mix-gas autoclaved

text: River delta

characteristic: environment (biome)

text: Sediment

characteristic: environment (feature)

text: Sediment

characteristic: environment (material)

text: Greenland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: Southwest Greenland, Watson River

text: metagenome

text: unidentified prokaryotic organism

characteristic: organism

text: 341F, 806R

characteristic: pcr primers

text: Watson river delta sediment incubations

characteristic: project name

text: unidentified prokaryotic organism

text: sediment

text: MiSeq

text: T1 (Day 0) Mix-gas autoclaved


text: UWASH

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: depth

text: T2 (Day 60) Control

characteristic: description

text: River delta

text: Sediment

text: Sediment

text: Greenland

characteristic: geographic location (elevation)

characteristic: geographic location (longitude)

text: Southwest Greenland, Watson River

text: metagenome

text: unidentified prokaryotic organism

text: 341F, 806R

text: Watson river delta sediment incubations

characteristic: sample volume or weight for DNA extraction

text: unidentified prokaryotic organism

text: sediment

characteristic: sediment environmental package

text: MiSeq

text: T2 (Day 60) Control

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UWASH

text: public

characteristic: collection date

text: T2 (Day 60) Control autoclaved

text: River delta

characteristic: environment (biome)

text: Sediment

characteristic: environment (feature)

text: Sediment

characteristic: environment (material)

text: Greenland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: Southwest Greenland, Watson River

characteristic: geographic location (region and locality)

text: metagenome

characteristic: investigation type

text: unidentified prokaryotic organism

text: 341F, 806R

text: Watson river delta sediment incubations

text: unidentified prokaryotic organism

text: sediment

text: MiSeq

characteristic: sequencing method

text: T2 (Day 60) Control autoclaved


text: UWASH

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: depth

text: T2 (Day 60) Methanol

characteristic: description

text: River delta

text: Sediment

text: Sediment

text: Greenland

characteristic: geographic location (elevation)

characteristic: geographic location (longitude)

text: Southwest Greenland, Watson River

text: metagenome

text: unidentified prokaryotic organism

characteristic: organism

text: 341F, 806R

characteristic: pcr primers

text: Watson river delta sediment incubations

characteristic: project name

text: unidentified prokaryotic organism

text: sediment

text: MiSeq

text: T2 (Day 60) Methanol


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UWASH

text: public

characteristic: collection date

text: T2 (Day 60) Methanol autoclaved

text: River delta

characteristic: environment (biome)

text: Sediment

characteristic: environment (feature)

text: Sediment

characteristic: environment (material)

text: Greenland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: Southwest Greenland, Watson River

text: metagenome

text: unidentified prokaryotic organism

text: 341F, 806R

text: Watson river delta sediment incubations

characteristic: sample volume or weight for DNA extraction

text: unidentified prokaryotic organism

text: sediment

characteristic: sediment environmental package

text: MiSeq

text: T2 (Day 60) Methanol autoclaved

characteristic: title


text: UWASH

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: depth

text: T2 (Day 60) Mix-gas

characteristic: description

text: River delta

text: Sediment

text: Sediment

text: Greenland

characteristic: geographic location (elevation)

characteristic: geographic location (longitude)

text: Southwest Greenland, Watson River

characteristic: geographic location (region and locality)

text: metagenome

characteristic: investigation type

text: unidentified prokaryotic organism

text: 341F, 806R

text: Watson river delta sediment incubations

text: unidentified prokaryotic organism

text: sediment

text: MiSeq

characteristic: sequencing method

text: T2 (Day 60) Mix-gas


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UWASH

text: public

characteristic: collection date

text: T2 (Day 60) Mix-gas autoclaved

text: River delta

characteristic: environment (biome)

text: Sediment

characteristic: environment (feature)

text: Sediment

characteristic: environment (material)

text: Greenland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: Southwest Greenland, Watson River

text: metagenome

text: unidentified prokaryotic organism

characteristic: organism

text: 341F, 806R

characteristic: pcr primers

text: Watson river delta sediment incubations

characteristic: project name

text: unidentified prokaryotic organism

text: sediment

text: MiSeq

text: T2 (Day 60) Mix-gas autoclaved


text: UWASH

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: depth

text: T3 (Day 115 or 147) Control

characteristic: description

text: River delta

text: Sediment

text: Sediment

text: Greenland

characteristic: geographic location (elevation)

characteristic: geographic location (longitude)

text: Southwest Greenland, Watson River

text: metagenome

text: unidentified prokaryotic organism

text: 341F, 806R

text: Watson river delta sediment incubations

characteristic: sample volume or weight for DNA extraction

text: unidentified prokaryotic organism

text: sediment

characteristic: sediment environmental package

text: MiSeq

text: T3 (Day 115 or 147) Control

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UWASH

text: public

characteristic: collection date

text: T3 (Day 115 or 147) Control autoclaved

text: River delta

characteristic: environment (biome)

text: Sediment

characteristic: environment (feature)

text: Sediment

characteristic: environment (material)

text: Greenland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: Southwest Greenland, Watson River

characteristic: geographic location (region and locality)

text: metagenome

characteristic: investigation type

text: unidentified prokaryotic organism

text: 341F, 806R

text: Watson river delta sediment incubations

text: unidentified prokaryotic organism

text: sediment

text: MiSeq

characteristic: sequencing method

text: T3 (Day 115 or 147) Control autoclaved


text: UWASH

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: depth

text: T3 (Day 115 or 147) Methanol

characteristic: description

text: River delta

text: Sediment

text: Sediment

text: Greenland

characteristic: geographic location (elevation)

characteristic: geographic location (longitude)

text: Southwest Greenland, Watson River

text: metagenome

text: unidentified prokaryotic organism

characteristic: organism

text: 341F, 806R

characteristic: pcr primers

text: Watson river delta sediment incubations

characteristic: project name

text: unidentified prokaryotic organism

text: sediment

text: MiSeq

text: T3 (Day 115 or 147) Methanol


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UWASH

text: public

characteristic: collection date

text: T3 (Day 115 or 147) Methanol autoclaved

text: River delta

characteristic: environment (biome)

text: Sediment

characteristic: environment (feature)

text: Sediment

characteristic: environment (material)

text: Greenland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: Southwest Greenland, Watson River

text: metagenome

text: unidentified prokaryotic organism

text: 341F, 806R

text: Watson river delta sediment incubations

characteristic: sample volume or weight for DNA extraction

text: unidentified prokaryotic organism

text: sediment

characteristic: sediment environmental package

text: MiSeq

text: T3 (Day 115 or 147) Methanol autoclaved

characteristic: title


text: UWASH

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: depth

text: T3 (Day 115 or 147) Mix-gas

characteristic: description

text: River delta

text: Sediment

text: Sediment

text: Greenland

characteristic: geographic location (elevation)

characteristic: geographic location (longitude)

text: Southwest Greenland, Watson River

characteristic: geographic location (region and locality)

text: metagenome

characteristic: investigation type

text: unidentified prokaryotic organism

text: 341F, 806R

text: Watson river delta sediment incubations

text: unidentified prokaryotic organism

text: sediment

text: MiSeq

characteristic: sequencing method

text: T3 (Day 115 or 147) Mix-gas


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UWASH

text: public

characteristic: collection date

text: T3 (Day 115 or 147) Mix-gas autoclaved

text: River delta

characteristic: environment (biome)

text: Sediment

characteristic: environment (feature)

text: Sediment

characteristic: environment (material)

text: Greenland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: Southwest Greenland, Watson River

text: metagenome

text: unidentified prokaryotic organism

characteristic: organism

text: 341F, 806R

characteristic: pcr primers

text: Watson river delta sediment incubations

characteristic: project name

text: unidentified prokaryotic organism

text: sediment

text: MiSeq

text: T3 (Day 115 or 147) Mix-gas autoclaved


text: UWASH

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: depth

text: T5 (Day 371) Control

characteristic: description

text: River delta

text: Sediment

text: Sediment

text: Greenland

characteristic: geographic location (elevation)

characteristic: geographic location (longitude)

text: Southwest Greenland, Watson River

text: metagenome

text: unidentified prokaryotic organism

text: 341F, 806R

text: Watson river delta sediment incubations

characteristic: sample volume or weight for DNA extraction

text: unidentified prokaryotic organism

text: sediment

characteristic: sediment environmental package

text: MiSeq

text: T5 (Day 371) Control

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UWASH

text: public

characteristic: collection date

text: T5 (Day 371) Control autoclaved

text: River delta

characteristic: environment (biome)

text: Sediment

characteristic: environment (feature)

text: Sediment

characteristic: environment (material)

text: Greenland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: Southwest Greenland, Watson River

characteristic: geographic location (region and locality)

text: metagenome

characteristic: investigation type

text: unidentified prokaryotic organism

text: 341F, 806R

text: Watson river delta sediment incubations

text: unidentified prokaryotic organism

text: sediment

text: MiSeq

characteristic: sequencing method

text: T5 (Day 371) Control autoclaved


text: UWASH

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: depth

text: T5 (Day 371) Methanol

characteristic: description

text: River delta

text: Sediment

text: Sediment

text: Greenland

characteristic: geographic location (elevation)

characteristic: geographic location (longitude)

text: Southwest Greenland, Watson River

text: metagenome

text: unidentified prokaryotic organism

characteristic: organism

text: 341F, 806R

characteristic: pcr primers

text: Watson river delta sediment incubations

characteristic: project name

text: unidentified prokaryotic organism

text: sediment

text: MiSeq

text: T5 (Day 371) Methanol


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UWASH

text: public

characteristic: collection date

text: T5 (Day 371) Methanol autoclaved

text: River delta

characteristic: environment (biome)

text: Sediment

characteristic: environment (feature)

text: Sediment

characteristic: environment (material)

text: Greenland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: Southwest Greenland, Watson River

text: metagenome

text: unidentified prokaryotic organism

text: 341F, 806R

text: Watson river delta sediment incubations

characteristic: sample volume or weight for DNA extraction

text: unidentified prokaryotic organism

text: sediment

characteristic: sediment environmental package

text: MiSeq

text: T5 (Day 371) Methanol autoclaved

characteristic: title


text: UWASH

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: depth

text: T5 (Day 371) Mix-gas

characteristic: description

text: River delta

text: Sediment

text: Sediment

text: Greenland

characteristic: geographic location (elevation)

characteristic: geographic location (longitude)

text: Southwest Greenland, Watson River

characteristic: geographic location (region and locality)

text: metagenome

characteristic: investigation type

text: unidentified prokaryotic organism

text: 341F, 806R

text: Watson river delta sediment incubations

text: unidentified prokaryotic organism

text: sediment

text: MiSeq

characteristic: sequencing method

text: T5 (Day 371) Mix-gas


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UWASH

text: public

characteristic: collection date

text: T5 (Day 371) Mix-gas autoclaved

text: River delta

characteristic: environment (biome)

text: Sediment

characteristic: environment (feature)

text: Sediment

characteristic: environment (material)

text: Greenland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: Southwest Greenland, Watson River

text: metagenome

text: unidentified prokaryotic organism

characteristic: organism

text: 341F, 806R

characteristic: pcr primers

text: Watson river delta sediment incubations

characteristic: project name

text: unidentified prokaryotic organism

text: sediment

text: MiSeq

text: T5 (Day 371) Mix-gas autoclaved


text: FRED HUTCHINSON CANCER RESEARCH CENTER

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:FRED HUTCHINSON CANCER RESEARCH CENTER

characteristic: Submitter Id

characteristic: amount or size of sample collected

text: house mouse

characteristic: common name

text: fecal samples collected from the same cage of mice after rapamaycin injection starting at 19-20 months old

characteristic: description

text: Foege building

text: Intestine environment(ENVO:2100002)

text: USA

characteristic: geographic location (longitude)

unit: month

characteristic: host age

text: colon

characteristic: host body habitat

text: house mouse

characteristic: host common name

text: Picolab Rodent Diet 20 #5053 (Lab Diet, St. Louis, MO)

characteristic: host diet

characteristic: host genotype

text: old

characteristic: host phenotype

text: female

characteristic: host sex

text: host-associated

characteristic: host-associated environmental package

text: metagenome

text: PCR

characteristic: nucleic acid amplification

text: isopropanol precipitation

characteristic: nucleic acid extraction

text: Mus musculus

text: PCR reactions (50 uL) contained 100 ng of genomic DNA, forward and reverse primers (0.2 uM each), dNTPs (200 uM each), and Phusion High-Fidelity DNA Polymerase (1U). PCR amplification was performed as follows: amplification at 98�C for 3 minutes, and 25 cycles at 98�C for denaturation for 15 seconds, annealing at 50�C for 30 seconds, and extension at 72�C for 30 seconds, then a final extension at 72�C for 7 minutes.

characteristic: pcr conditions

text: Universal primers (U515F/806R)

text: mouse gut microbiome change by rapamycin

characteristic: sample volume or weight for DNA extraction

text: Mus musculus

text: Illumina MiSeq

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: FRED HUTCHINSON CANCER RESEARCH CENTER

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

text: rapamycin 8 mg/kg/daily i.p. injection in 1.2 mg/ml rapamycin/5% PEG-400/5% Tween20/ 1.2% DMSO, for 3 months

characteristic: chemical administration

characteristic: collection date

text: house mouse

text: fecal samples collected from the same cage of mice after vehicle injection starting at 19-20 months old

text: Foege building

characteristic: environment (biome)

text: Intestine environment(ENVO:2100002)

characteristic: environment (feature)

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: colon

text: house mouse

text: old

text: female

text: host-associated

text: metagenome

characteristic: investigation type

text: PCR

text: isopropanol precipitation

text: Mus musculus

characteristic: organism

text: Universal primers (U515F/806R)

characteristic: pcr primers

characteristic: project name

text: Mus musculus

text: Illumina MiSeq

characteristic: sequencing method


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: FRED HUTCHINSON CANCER RESEARCH CENTER

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: equivalent volume of daily i.p. injection of 5% PEG-400/5% Tween20/ 1.2% DMSO, for 3 months

text: house mouse

characteristic: common name

characteristic: description

text: Foege building

text: Intestine environment(ENVO:2100002)

text: USA

characteristic: geographic location (longitude)

characteristic: host age

text: colon

characteristic: host body habitat

text: house mouse

characteristic: host common name

characteristic: host diet

characteristic: host genotype

text: old

characteristic: host phenotype

text: female

characteristic: host sex

text: host-associated

characteristic: host-associated environmental package

text: metagenome

text: PCR

characteristic: nucleic acid amplification

text: isopropanol precipitation

characteristic: nucleic acid extraction

text: Mus musculus

characteristic: pcr conditions

text: Universal primers (U515F/806R)

characteristic: sample volume or weight for DNA extraction

text: Mus musculus

text: Illumina MiSeq

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: FRED HUTCHINSON CANCER RESEARCH CENTER

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: amount or size of sample collected

characteristic: chemical administration

characteristic: collection date

text: house mouse

text: fecal samples collected from the same cage of mice after rapamaycin feeding starting at 24 months old

text: Foege building

characteristic: environment (biome)

text: Intestine environment(ENVO:2100002)

characteristic: environment (feature)

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: colon

text: house mouse

text: Picolab Rodent Diet 20 #5053 (Lab Diet, St. Louis, MO) & 0.3 % agar

text: old

text: female

text: host-associated

text: metagenome

characteristic: investigation type

text: PCR

text: isopropanol precipitation

text: Mus musculus

characteristic: organism

text: Universal primers (U515F/806R)

characteristic: pcr primers

characteristic: project name

text: Mus musculus

text: Illumina MiSeq

characteristic: sequencing method


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: FRED HUTCHINSON CANCER RESEARCH CENTER

characteristic: INSDC center name

characteristic: INSDC first public

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text: public

text: feeding food containing encapsulated rapamycin at 126 ppm and 0.3% agar for 3 months

text: house mouse

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text: fecal samples collected from the same cage of mice after control food feeding starting at 24 months old

characteristic: description

text: Foege building

text: Intestine environment(ENVO:2100002)

text: USA

characteristic: geographic location (longitude)

characteristic: host age

text: colon

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text: house mouse

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characteristic: host diet

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text: old

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text: female

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text: host-associated

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text: metagenome

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characteristic: nucleic acid amplification

text: isopropanol precipitation

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text: Mus musculus

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characteristic: sample volume or weight for DNA extraction

text: Mus musculus

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characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

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characteristic: INSDC center name

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characteristic: SRA accession

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text: feeding food containing equivalent amount of capsules and 0.3% agar for 3 months

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text: house mouse

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characteristic: environment (biome)

text: Intestine environment(ENVO:2100002)

characteristic: environment (feature)

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characteristic: sequencing method


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text: FRED HUTCHINSON CANCER RESEARCH CENTER

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characteristic: title


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text: FRED HUTCHINSON CANCER RESEARCH CENTER

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text: house mouse

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characteristic: environment (biome)

text: Intestine environment(ENVO:2100002)

characteristic: environment (feature)

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: colon

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text: 26_14C_cont_ food_F_4th_24M_3M_NL


characteristic: ENA-CHECKLIST

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text: FRED HUTCHINSON CANCER RESEARCH CENTER

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characteristic: geographic location (longitude)

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text: house mouse

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text: old

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text: female

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text: FRED HUTCHINSON CANCER RESEARCH CENTER

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text: feeding food containing encapsulated rapamycin at 378 ppm and 0.3% agar for 6 months

characteristic: chemical administration

characteristic: collection date

text: house mouse

text: fecal samples collected from the same cage of mice after control food feeding starting at 3 months old for 6 months

text: Foege building

characteristic: environment (biome)

text: Intestine environment(ENVO:2100002)

characteristic: environment (feature)

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: colon

text: house mouse

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text: 28_15C_cont_ food27x_F_5th_24M_6M_TI


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text: public

text: feeding food containing equivalent amount of capsules and 0.3% agar for 6 months

text: house mouse

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text: fecal samples collected from the same cage of mice after rapamaycin feeding starting at 19-20 months old

characteristic: description

text: Foege building

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text: USA

characteristic: geographic location (longitude)

characteristic: host age

text: colon

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text: house mouse

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text: old

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text: female

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text: host-associated

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text: isopropanol precipitation

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text: Mus musculus

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text: 29_16R_rapa_food_F_6th_ 20M_3M_AB

characteristic: title


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text: FRED HUTCHINSON CANCER RESEARCH CENTER

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characteristic: environment (biome)

text: Intestine environment(ENVO:2100002)

characteristic: environment (feature)

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

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text: house mouse

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characteristic: organism

text: PCR reactions (50 uL) contained 100 ng of genomic DNA, forward and reverse primers (0.2 uM each), dNTPs (200 uM each), and Phusion High-Fidelity DNA Polymerase (1U). PCR amplification was performed as follows: amplification at 98�C for 3 minutes, and 25 cycles at 98�C for denaturation for 15 seconds, annealing at 50�C for 30 seconds, and extension at 72�C for 30 seconds, then a final extension at 72�C for 7 minutes.

text: Universal primers (U515F/806R)

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text: Mus musculus

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text: 30_16C_cont_food_F_6th_ 20M_3M_AB


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text: FRED HUTCHINSON CANCER RESEARCH CENTER

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characteristic: geographic location (longitude)

characteristic: host age

text: colon

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text: house mouse

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text: Mus musculus

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text: mouse gut microbiome change by rapamycin

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text: Mus musculus

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text: 31_17R_rapa_food_F_6th_ 20M_3M_AB

characteristic: title


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characteristic: ENA-LAST-UPDATE

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text: FRED HUTCHINSON CANCER RESEARCH CENTER

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text: house mouse

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text: Intestine environment(ENVO:2100002)

characteristic: environment (feature)

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

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text: 32_17C_cont_food_F_6th_ 20M_3M_AB


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characteristic: ENA-LAST-UPDATE

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text: FRED HUTCHINSON CANCER RESEARCH CENTER

characteristic: INSDC center name

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characteristic: geographic location (longitude)

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characteristic: host body habitat

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characteristic: host common name

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characteristic: host-associated environmental package

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characteristic: sample volume or weight for DNA extraction

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text: 33_18R_rapa_food_M_6th_ 20M_3M_AB

characteristic: title


characteristic: ENA-CHECKLIST

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characteristic: ENA-LAST-UPDATE

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text: FRED HUTCHINSON CANCER RESEARCH CENTER

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characteristic: Submitter Id

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text: house mouse

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characteristic: environment (biome)

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characteristic: environment (feature)

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: colon

text: house mouse

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characteristic: pcr primers

characteristic: project name

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text: 34_18C_cont_food_M_6th_ 20M_3M_AB


characteristic: ENA-CHECKLIST

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characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: FRED HUTCHINSON CANCER RESEARCH CENTER

characteristic: INSDC center name

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characteristic: geographic location (longitude)

characteristic: host age

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characteristic: host body habitat

text: house mouse

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text: old

characteristic: host phenotype

text: female

characteristic: host sex

text: host-associated

characteristic: host-associated environmental package

text: metagenome

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characteristic: nucleic acid amplification

text: isopropanol precipitation

characteristic: nucleic acid extraction

text: Mus musculus

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text: Universal primers (U515F/806R)

characteristic: sample volume or weight for DNA extraction

text: Mus musculus

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text: 35_19R_rapa_food_M_6th_ 20M_3M_AB

characteristic: title


characteristic: ENA-CHECKLIST

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characteristic: ENA-LAST-UPDATE

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text: FRED HUTCHINSON CANCER RESEARCH CENTER

characteristic: INSDC center name

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characteristic: Submitter Id

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text: Intestine environment(ENVO:2100002)

characteristic: environment (feature)

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

text: colon

text: house mouse

text: old

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text: host-associated

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characteristic: investigation type

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text: isopropanol precipitation

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text: 36_19C_cont_food_M_6th_ 20M_3M_AB


characteristic: ENA-CHECKLIST

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characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: altitude

characteristic: anonymized name

text: False

text: food metagenome

characteristic: common name

text: GAZ:United States of America

characteristic: country

characteristic: depth

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text: True

characteristic: elevation

text: ENVO:cropland biome

characteristic: env biome

text: ENVO:vineyard

characteristic: env feature

text: ENVO:wine

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text: misc environment

characteristic: env package

text: True

characteristic: extracted DNA avail now

text: miscellaneous metagenome

characteristic: host common name

characteristic: host subject id

characteristic: host taxid

characteristic: latitude

characteristic: longitude

text: food metagenome

text: True

characteristic: physical samp avail now

text: UC Davis

text: True

text: True

characteristic: public

text: Oakville

characteristic: region

characteristic: rep

text: completed

text: wine

characteristic: sample site

text: wine

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text: E

characteristic: stage

characteristic: title

text: Microbiota of regional grapes and wine fermentations

characteristic: variety

characteristic: vineyard

text: NN

characteristic: winery


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

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characteristic: SRA accession

characteristic: Submitter Id

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characteristic: anonymized name

text: False

characteristic: collection date

text: food metagenome

characteristic: common name

text: GAZ:United States of America

characteristic: country

characteristic: depth

characteristic: description

text: True

characteristic: elevation

text: ENVO:cropland biome

characteristic: env biome

text: ENVO:vineyard

characteristic: env feature

text: ENVO:must

characteristic: env matter

text: misc environment

characteristic: env package

text: True

characteristic: extracted DNA avail now

text: miscellaneous metagenome

text: food metagenome

characteristic: organism

text: True

text: UC Davis

text: True

text: True

text: Oakville

text: completed

text: must

text: must

text: food metagenome

text: B

text: NN


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

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characteristic: INSDC first public

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text: food metagenome

text: GAZ:United States of America

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text: ENVO:cropland biome

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text: True

text: miscellaneous metagenome

characteristic: host common name

characteristic: host subject id

characteristic: host taxid

characteristic: latitude

characteristic: longitude

text: food metagenome

text: True

characteristic: physical samp avail now

text: UC Davis

text: True

text: True

characteristic: public

text: Coombsville

characteristic: region

characteristic: rep

text: completed

text: wine

characteristic: sample site

text: wine

characteristic: sample type

text: food metagenome

text: F

characteristic: stage

characteristic: title

text: Chardonnay

characteristic: variety

characteristic: vineyard

text: FN

characteristic: winery


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

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characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

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characteristic: collection date

text: food metagenome

characteristic: common name

text: GAZ:United States of America

characteristic: country

characteristic: depth

characteristic: description

text: True

characteristic: elevation

text: ENVO:cropland biome

characteristic: env biome

text: ENVO:vineyard

characteristic: env feature

text: ENVO:wine

characteristic: env matter

text: misc environment

characteristic: env package

text: True

characteristic: extracted DNA avail now

text: miscellaneous metagenome

text: food metagenome

characteristic: organism

text: True

text: UC Davis

text: True

text: True

text: Oakville

text: completed

text: wine

text: wine

text: food metagenome

text: F

text: Chardonnay

text: FN


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

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text: False

text: food metagenome

text: GAZ:United States of America

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text: ENVO:cropland biome

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text: misc environment

text: True

text: miscellaneous metagenome

characteristic: host common name

characteristic: host subject id

characteristic: host taxid

characteristic: latitude

characteristic: longitude

text: food metagenome

text: True

characteristic: physical samp avail now

text: UC Davis

text: True

text: True

characteristic: public

text: Oakville

characteristic: region

characteristic: rep

text: completed

text: must

characteristic: sample site

text: must

characteristic: sample type

text: food metagenome

text: B

characteristic: stage

characteristic: title

text: Dolce

characteristic: variety

text: Dolce

characteristic: vineyard

text: DOLCE

characteristic: winery


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

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characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: altitude

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