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characteristic: External Id

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characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: n

characteristic: antiexposedall

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text: bd

characteristic: diet

text: bd

text: eb

text: True

text: urban biome

text: human-associated habitat

text: USA:NY:NYC

characteristic: host subject id

characteristic: latitude

text: C

text: False

text: False

text: human gut metagenome

text: NYUMC

text: True

characteristic: sample summary

text: human gut metagenome

text: Male

characteristic: title


text: UCSDMI

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: Moxifloxacin

text: post

text: y

characteristic: day of life

text: Vaginal

characteristic: delivery

characteristic: description

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text: bd

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characteristic: elevation

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characteristic: env biome

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characteristic: geo loc name

characteristic: longitude

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text: False

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characteristic: month

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characteristic: organism

text: NYUMC

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characteristic: sample type

characteristic: sampletype

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text: Male


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

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text: Amoxicillin

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characteristic: antiexposedall

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characteristic: diet

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text: fd

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text: urban biome

text: human-associated habitat

text: USA:NY:NYC

characteristic: host subject id

characteristic: latitude

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text: True

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text: human gut metagenome

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characteristic: sex

characteristic: studyid


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characteristic: SRA accession

characteristic: Submitter Id

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text: Amoxicillin

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characteristic: geo loc name

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characteristic: sample summary

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characteristic: title


characteristic: ENA-CHECKLIST

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characteristic: External Id

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characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

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characteristic: host subject id

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characteristic: host subject id

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text: False

text: human gut metagenome

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characteristic: sample type

characteristic: sampletype

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text: UCSDMI

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text: Amoxicillin

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characteristic: host subject id

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text: False

text: human gut metagenome

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text: UCSDMI

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text: human gut metagenome

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text: UCSDMI

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text: Amoxicillin

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text: True

text: urban biome

text: human-associated habitat

text: USA:NY:NYC

characteristic: host subject id

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text: C

text: False

text: False

text: human gut metagenome

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text: True

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text: human gut metagenome

text: Female

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text: UCSDMI

text: public

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text: n

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text: Cesarean

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text: fd

text: True

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text: urban biome

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text: human-associated habitat

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text: USA:NY:NYC

characteristic: geo loc name

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text: True

text: False

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text: human gut metagenome

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text: True

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characteristic: sampletype

text: human gut metagenome

text: Female


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characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

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text: public

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text: n

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text: bd

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text: bd

text: eb

text: True

text: urban biome

text: human-associated habitat

text: USA:NY:NYC

characteristic: host subject id

characteristic: latitude

text: C

text: False

text: False

text: human gut metagenome

text: NYUMC

text: True

text: human gut metagenome

text: Male

characteristic: sex

characteristic: studyid


text: UCSDMI

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: n

characteristic: day of life

text: Cesarean

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characteristic: description

text: fd

text: fd

text: fd

text: True

characteristic: elevation

text: urban biome

characteristic: env biome

text: human-associated habitat

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text: USA:NY:NYC

characteristic: geo loc name

characteristic: longitude

text: C

text: True

text: False

characteristic: month

text: human gut metagenome

characteristic: organism

text: NYUMC

text: True

characteristic: sample summary

text: human gut metagenome

text: Male

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: n

characteristic: antiexposedall

text: Cesarean

text: fd

characteristic: diet

text: fd

text: fd

text: True

text: urban biome

text: human-associated habitat

text: USA:NY:NYC

characteristic: host subject id

characteristic: latitude

text: C

text: True

text: False

text: human gut metagenome

text: NYUMC

text: True

characteristic: sample type

characteristic: sampletype

text: human gut metagenome

text: Male


text: UCSDMI

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: pre

text: Cephalexin

text: pre

text: n

characteristic: day of life

text: Vaginal

characteristic: delivery

characteristic: description

text: bd

text: bd

text: eb

text: True

characteristic: elevation

text: urban biome

characteristic: env biome

text: human-associated habitat

characteristic: env feature

text: USA:NY:NYC

characteristic: geo loc name

characteristic: longitude

text: C

text: False

text: False

characteristic: month

text: human gut metagenome

characteristic: organism

text: NYUMC

text: True

text: human gut metagenome

text: Male

characteristic: sex

characteristic: studyid


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: n

characteristic: antiexposedall

text: Vaginal

text: fd

characteristic: diet

text: fd

text: fd

text: True

text: urban biome

text: human-associated habitat

text: USA:NY:NYC

characteristic: host subject id

characteristic: latitude

text: C

text: False

text: True

text: beta-lactam

text: human gut metagenome

text: NYUMC

text: True

characteristic: sample summary

text: human gut metagenome

text: Male

characteristic: title


text: UCSDMI

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: n

characteristic: day of life

text: Vaginal

characteristic: delivery

characteristic: description

text: fd

text: fd

text: fd

text: True

characteristic: elevation

text: urban biome

characteristic: env biome

text: human-associated habitat

characteristic: env feature

text: USA:NY:NYC

characteristic: geo loc name

characteristic: longitude

text: C

text: False

text: False

characteristic: month

text: human gut metagenome

characteristic: organism

text: NYUMC

text: True

characteristic: sample type

characteristic: sampletype

text: human gut metagenome

text: Female


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INSTITUTE FOR MOLECULAR BIOSCIENCE UQ

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

tag: Namespace:INSTITUTE FOR MOLECULAR BIOSCIENCE UQ

characteristic: collection date

text: USA

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: host health state

text: human

characteristic: host scientific name

text: clinical

characteristic: isolate

text: human urine

characteristic: isolation source

text: Escherichia coli

text: Escherichia coli

text: FDA strain Seattle 1946 [DSM 1103, NCIB 12210]

characteristic: serovar

text: Escherichia coli ATCC-25922

characteristic: strain

text: MiSEQ Escherichia coli


text: INSTITUTE FOR MOLECULAR BIOSCIENCE UQ

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: USA

text: not provided

text: human

text: clinical

text: human urine

text: Staphylococcus aureus subsp. aureus

characteristic: organism

text: Staphylococcus aureus subsp. aureus

text: Rosenbach, Seattle 1945

text: Staphylococcus aureus supse. aureus ATCC-25923

text: MiSEQ Staphylococcus aureus

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Wellcome Trust Sanger Institute

text: public

tag: Namespace:Wellcome Trust Sanger Institute

text: 9 to 16

unit: week

characteristic: age

text: ArrayExpress

characteristic: broker name

text: lymphocyte of B lineage

characteristic: cell type

text: house mouse

characteristic: common name

text: Protocols: Splenic B cells were isolated from mice aged 9-16wks by depletion of CD43+ cells 65 using magnetic microbeads (Miltenyi Biotec, Cat no. 130-049-801). Purity of the isolated B cells was assessed using anti-B220 antibody (clone RA3-6B2) and found to be >98% for B220+ cells. To induce class-switching to IgG1 and IgE, B cells were seeded into 96-well round bottom plates at a density of 105 cells/200 μl RPMI supplemented with 10% FBS, 50 μM 2-mercaptoethanol, 100 U/ml penicillin- 100 μg/ml streptomycin (Invitrogen; Cat no. 15140122), 25 ng/ml recombinant mouse IL-4 (R&D Systems; Cat no. 404-ML), 40 μg/ml LPS (Sigma-Aldrich; L4391 or L3129) and 20 ng/ml recombinant mouse BAFF of (R&DSystems; Cat no. 2106-BF). Cells were harvested after 24-hours for FACS as detailed in the cell-lysis protocol\. After treatment as described in the stimulation protocol, single cells were deposited into 96-well PCR plates containing lysis buffer using an iCyt Synergy cell sorter. Plates were then immediately spun down and stored at -80°C until further processing. The generation of the single-cell libraries followed the Smart-seq2 protocol42, then switched to the Fluidigm C1 protocol for tagmentation and purification of the pooled libraries (Fluidigm; PN 100-7168). The pooled, barcoded libraries were quantified by qPCR and checked for size distribution using an Agilent Bioanalyzer 2100.

characteristic: description

text: I1-eGFP x I-tdTom

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: eGFP positive

characteristic: phenotype

text: Mus musculus

text: male

characteristic: sex

text: Interleukin 4, lipopolysaccharide, B cell activating factor

characteristic: stimulus

text: BALB/c


text: Wellcome Trust Sanger Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: 9 to 16

text: ArrayExpress

text: lymphocyte of B lineage

text: house mouse

text: I1-eGFP x I-tdTom

text: Mus musculus

text: Mus musculus

text: tdTomato positive

text: Mus musculus

text: male

text: BALB/c

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Wellcome Trust Sanger Institute

text: public

characteristic: Submitter Id

text: 9 to 16

text: ArrayExpress

text: lymphocyte of B lineage

text: house mouse

text: I1-eGFP x I-tdTom

text: Mus musculus

characteristic: organism

text: Mus musculus

text: eGFP positive

text: Mus musculus

text: female

text: BALB/c

characteristic: title


characteristic: External Id

text: Wellcome Trust Sanger Institute

text: public

text: 9 to 16

characteristic: age

text: ArrayExpress

characteristic: broker name

text: lymphocyte of B lineage

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

text: I1-eGFP x I-tdTom

text: Mus musculus

text: Mus musculus

text: no fluorescence

text: Mus musculus

text: female

text: BALB/c


text: Wellcome Trust Sanger Institute

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: 9 to 16

text: ArrayExpress

text: lymphocyte of B lineage

text: house mouse

text: I1-eGFP x I-tdTom

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: tdTomato positive

characteristic: phenotype

text: Mus musculus

text: female

characteristic: sex

characteristic: stimulus

text: BALB/c


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: BANG

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Syphacia obvelata

text: Syphacia obvelata

text: Syphacia obvelata from Mus musculus domesticus


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: WSL BIRMENSDORF

text: public

tag: Namespace:WSL BIRMENSDORF

characteristic: Submitter Id

characteristic: collection date

unit: m

characteristic: depth

text: Gerlafingen soil, 0 Hg ug g-1

text: Forest

characteristic: environment (biome)

text: Forest soil

characteristic: environment (feature)

text: Soil

characteristic: environment (material)

text: Switzerland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (elevation)

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: soil metagenome

characteristic: organism

characteristic: ph

text: Hg levels in Swiss forest soils

characteristic: project name

text: soil metagenome

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: Bacteria

characteristic: title

unit: µg/L

characteristic: total nitrogen

unit: g/kg

characteristic: total organic carbon


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: WSL BIRMENSDORF

characteristic: INSDC center name

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