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text: Impact of fire on active layer and permafrost microbial communities and metagenomes in an upland Alaskan boreal forest


text: UCSDMI

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text: qiita_sid_1030:1030.TVF.2010.Loc3.D0.Rep1

text: FALSE

text: soil metagenome

characteristic: common name

text: GAZ:United States of America

characteristic: country

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text: Tanana Valley Forest Fire year 2010 Organic layer Location03 Replicate01

characteristic: description

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text: qiita_sid_1030:1030.TVF.2010.Loc3.D0.Rep2

characteristic: Submitter Id

characteristic: altitude

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text: FALSE

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text: Tanana Valley Forest Fire year 2010 Organic layer Location03 Replicate02

text: TRUE

text: forest biome

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characteristic: latitude

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text: FALSE

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characteristic: common name

text: GAZ:United States of America

characteristic: country

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text: Tanana Valley Forest Fire year 2010 Organic layer Location03 Replicate03

characteristic: description

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characteristic: ENA-CHECKLIST

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characteristic: SRA accession

text: qiita_sid_1030:1030.TVF.2010.Loc3.D0.Rep4

characteristic: Submitter Id

characteristic: altitude

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text: FALSE

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text: Tanana Valley Forest Fire year 2010 Organic layer Location03 Replicate04

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text: forest biome

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text: FALSE

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characteristic: common name

text: GAZ:United States of America

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text: Tanana Valley Forest Fire year 2010 Organic layer Location03 Replicate05

characteristic: description

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text: qiita_sid_1030:1030.TVF.2010.Loc3.D10.Rep1

characteristic: Submitter Id

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text: FALSE

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text: Tanana Valley Forest Fire year 2010 Mineral layer Location03 Replicate01

text: TRUE

text: forest biome

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text: UCSDMI

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text: qiita_sid_1030:1030.TVF.2010.Loc3.D10.Rep2

text: FALSE

text: soil metagenome

characteristic: common name

text: GAZ:United States of America

characteristic: country

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text: Tanana Valley Forest Fire year 2010 Mineral layer Location03 Replicate02

characteristic: description

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text: qiita_sid_1030:1030.TVF.2010.Loc3.D10.Rep3

characteristic: Submitter Id

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text: Tanana Valley Forest Fire year 2010 Mineral layer Location03 Replicate03

text: TRUE

text: forest biome

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text: qiita_sid_1030:1030.TVF.2010.Loc3.D10.Rep4

text: FALSE

text: soil metagenome

characteristic: common name

text: GAZ:United States of America

characteristic: country

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text: Tanana Valley Forest Fire year 2010 Mineral layer Location03 Replicate04

characteristic: description

text: TRUE

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text: forest soil

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text: soil

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characteristic: extracted DNA avail now

characteristic: fire

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text: soil metagenome

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text: TRUE

text: TRUE

characteristic: public

text: completed

text: soil

characteristic: sample type

text: sequenced (16S)

text: soil metagenome

text: Impact of fire on active layer and permafrost microbial communities and metagenomes in an upland Alaskan boreal forest


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

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text: qiita_sid_1030:1030.TVF.2010.Loc3.D10.Rep5

characteristic: Submitter Id

characteristic: altitude

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text: FALSE

text: soil metagenome

text: GAZ:United States of America

text: Tanana Valley Forest Fire year 2010 Mineral layer Location03 Replicate05

text: TRUE

text: forest biome

text: forest soil

text: soil

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text: UCSDMI

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text: qiita_sid_1030:1030.TVF.2010.Loc4.D0.Rep1

text: FALSE

text: soil metagenome

characteristic: common name

text: GAZ:United States of America

characteristic: country

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text: Tanana Valley Forest Fire year 2010 Organic layer Location04 Replicate01

characteristic: description

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characteristic: elevation

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characteristic: extracted DNA avail now

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text: soil metagenome

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characteristic: ENA-CHECKLIST

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text: qiita_sid_1030:1030.TVF.2010.Loc4.D0.Rep2

characteristic: Submitter Id

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text: FALSE

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text: Tanana Valley Forest Fire year 2010 Organic layer Location04 Replicate02

text: TRUE

text: forest biome

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text: soil

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text: sequenced (16S)

text: soil metagenome


text: UCSDMI

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text: qiita_sid_1030:1030.TVF.2010.Loc4.D0.Rep3

text: FALSE

text: soil metagenome

characteristic: common name

text: GAZ:United States of America

characteristic: country

characteristic: depth

text: Tanana Valley Forest Fire year 2010 Organic layer Location04 Replicate03

characteristic: description

text: TRUE

characteristic: elevation

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text: forest soil

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text: soil

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characteristic: extracted DNA avail now

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text: qiita_sid_1030:1030.TVF.2010.Loc4.D0.Rep4

characteristic: Submitter Id

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text: FALSE

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text: Tanana Valley Forest Fire year 2010 Organic layer Location04 Replicate04

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text: forest biome

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text: soil metagenome

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text: sequenced (16S)

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text: UCSDMI

text: public

text: qiita_sid_1030:1030.TVF.2010.Loc4.D0.Rep5

text: FALSE

text: soil metagenome

characteristic: common name

text: GAZ:United States of America

characteristic: country

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text: Tanana Valley Forest Fire year 2010 Organic layer Location04 Replicate05

characteristic: description

text: TRUE

characteristic: elevation

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text: forest soil

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text: soil

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text: TRUE

characteristic: public

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text: soil

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text: sequenced (16S)

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text: UCSDMI

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text: qiita_sid_1030:1030.TVF.2010.Loc4.D10.Rep1

characteristic: Submitter Id

characteristic: altitude

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text: FALSE

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text: Tanana Valley Forest Fire year 2010 Mineral layer Location04 Replicate01

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text: forest biome

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text: UCSDMI

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text: qiita_sid_1030:1030.TVF.2010.Loc4.D10.Rep2

text: FALSE

text: soil metagenome

characteristic: common name

text: GAZ:United States of America

characteristic: country

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text: Tanana Valley Forest Fire year 2010 Mineral layer Location04 Replicate02

characteristic: description

text: TRUE

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text: soil

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characteristic: extracted DNA avail now

characteristic: fire

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text: soil metagenome

text: CCME

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characteristic: ENA-CHECKLIST

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text: qiita_sid_1030:1030.TVF.2010.Loc4.D10.Rep3

characteristic: Submitter Id

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text: FALSE

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text: Tanana Valley Forest Fire year 2010 Mineral layer Location04 Replicate03

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text: forest biome

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characteristic: public

text: completed

text: soil

characteristic: sample type

text: sequenced (16S)

text: soil metagenome

text: Impact of fire on active layer and permafrost microbial communities and metagenomes in an upland Alaskan boreal forest


text: UCSDMI

text: public

text: qiita_sid_1030:1030.TVF.2010.Loc4.D10.Rep4

text: FALSE

text: soil metagenome

characteristic: common name

text: GAZ:United States of America

characteristic: country

characteristic: depth

text: Tanana Valley Forest Fire year 2010 Mineral layer Location04 Replicate04

characteristic: description

text: TRUE

characteristic: elevation

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text: forest soil

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text: soil

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characteristic: extracted DNA avail now

characteristic: fire

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text: qiita_sid_1030:1030.TVF.2010.Loc4.D10.Rep5

characteristic: Submitter Id

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text: FALSE

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text: Tanana Valley Forest Fire year 2010 Mineral layer Location04 Replicate05

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text: forest biome

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characteristic: latitude

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text: soil metagenome

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text: sequenced (16S)

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text: UCSDMI

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text: qiita_sid_1030:1030.TVF.2010.Loc5.D0.Rep1

text: FALSE

text: soil metagenome

characteristic: common name

text: GAZ:United States of America

characteristic: country

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text: Tanana Valley Forest Fire year 2010 Organic layer Location05 Replicate01

characteristic: description

text: TRUE

characteristic: elevation

text: forest biome

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text: forest soil

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text: soil

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characteristic: extracted DNA avail now

characteristic: fire

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text: soil

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text: sequenced (16S)

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characteristic: ENA-CHECKLIST

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text: qiita_sid_1030:1030.TVF.2010.Loc5.D0.Rep2

characteristic: Submitter Id

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text: FALSE

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text: forest biome

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text: FALSE

text: soil metagenome

characteristic: common name

text: GAZ:United States of America

characteristic: country

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text: Tanana Valley Forest Fire year 2010 Organic layer Location05 Replicate03

characteristic: description

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text: qiita_sid_1030:1030.TVF.2010.Loc5.D0.Rep4

characteristic: Submitter Id

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text: Tanana Valley Forest Fire year 2010 Organic layer Location05 Replicate04

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text: qiita_sid_1030:1030.TVF.2010.Loc5.D0.Rep5

text: FALSE

text: soil metagenome

characteristic: common name

text: GAZ:United States of America

characteristic: country

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text: Tanana Valley Forest Fire year 2010 Organic layer Location05 Replicate05

characteristic: description

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text: qiita_sid_1030:1030.TVF.2010.Loc5.D10.Rep1

characteristic: Submitter Id

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text: FALSE

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text: Tanana Valley Forest Fire year 2010 Mineral layer Location05 Replicate01

text: TRUE

text: forest biome

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text: UCSDMI

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text: qiita_sid_1030:1030.TVF.2010.Loc5.D10.Rep2

text: FALSE

text: soil metagenome

characteristic: common name

text: GAZ:United States of America

characteristic: country

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text: Tanana Valley Forest Fire year 2010 Mineral layer Location05 Replicate02

characteristic: description

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text: forest soil

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text: soil

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characteristic: extracted DNA avail now

characteristic: fire

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characteristic: public

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characteristic: sample type

text: sequenced (16S)

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text: Impact of fire on active layer and permafrost microbial communities and metagenomes in an upland Alaskan boreal forest


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

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characteristic: SRA accession

text: qiita_sid_1030:1030.TVF.2010.Loc5.D10.Rep3

characteristic: Submitter Id

characteristic: altitude

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text: FALSE

text: soil metagenome

text: GAZ:United States of America

text: Tanana Valley Forest Fire year 2010 Mineral layer Location05 Replicate03

text: TRUE

text: forest biome

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text: UCSDMI

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text: qiita_sid_1030:1030.TVF.2010.Loc5.D10.Rep4

text: FALSE

text: soil metagenome

characteristic: common name

text: GAZ:United States of America

characteristic: country

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text: Tanana Valley Forest Fire year 2010 Mineral layer Location05 Replicate04

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text: UCSDMI

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text: FALSE

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text: Impact of fire on active layer and permafrost microbial communities and metagenomes in an upland Alaskan boreal forest


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text: Impact of fire on active layer and permafrost microbial communities and metagenomes in an upland Alaskan boreal forest


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text: Impact of fire on active layer and permafrost microbial communities and metagenomes in an upland Alaskan boreal forest


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text: qiita_sid_1030:1030.TVF.Control.Loc5.D10.Rep3

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text: GAZ:United States of America

characteristic: country

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text: Tanana Valley Forest Fire year 2010 Mineral layer Location05 Replicate06

characteristic: description

text: TRUE

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text: soil

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characteristic: title


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text: qiita_sid_1030:1030.TVF.Control.Loc5.D10.Rep5

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text: FALSE

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text: Tanana Valley Forest Fire year 2010 Mineral layer Location05 Replicate07

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text: soil

text: soil

text: soil

text: TRUE

characteristic: latitude

characteristic: longitude

text: soil metagenome

text: CCME

text: TRUE

text: TRUE

text: completed

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text: NORWEGIAN VETERINARY INSTITUTE

text: public

characteristic: collection date

text: Oil drilling waste recycling plant

characteristic: environment (biome)

text: Oil drilling waste basin

characteristic: environment (feature)

text: Oil drilling wastewater sludge

characteristic: environment (material)

text: Norway

characteristic: geographic location (country and/or sea)

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: metagenome

text: Exposure for chemical and biological agents during cleaning and recycling of drilling waste

characteristic: project name

text: metagenome

text: Illumina (Miseq)

characteristic: sequencing method

text: Metagenome analysis of oil drilling waste at an oil drilling waste recycling plant

text: wastewater/sludge

characteristic: wastewater/sludge environmental package


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Instituto de Medicina Molecular

characteristic: INSDC center name

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text: public

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characteristic: SRA accession

tag: Namespace:Instituto de Medicina Molecular

text: ArrayExpress

characteristic: broker name

text: AnTat 1.1E 90-13

characteristic: cell line

text: Protocols: Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr at 32°C and 12 hr at 37°C. On the 4th and 5th days, parasites were kept in either these alternating conditions or at constant 37°C. Bloodstream forms were grown routinely in HMI-11 at 37°C in 5% CO2. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout two days. Temperature was varied every 12 hr from 32°C to 37°C or remained at constant 37°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer’s instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer’s instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina).

characteristic: description

text: Bloodstream form

characteristic: developmental stage

text: Trypanosoma brucei

characteristic: organism

text: Trypanosoma brucei

text: Trypanosoma brucei

text: Alternating temperature

characteristic: stimulus

text: AnTat 1.1E

characteristic: strain

unit: hour

characteristic: time

characteristic: title

type: has member