IXBr

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: IRIS

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: IXBr

text: Mesocosm I (Control)), May, Bottom layer

text: marine

text: Arctic

text: sea-ice

text: Norway

text: metatranscriptome

text: uncultured bacterium

text: OGP

characteristic: project name

text: uncultured bacterium

text: pyrosequencing

characteristic: sequencing method

characteristic: title

text: water

characteristic: water environmental package


E-MTAB-5062:Condition #1, Replicate #1

status: PUBLIC

tag: attribute

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

characteristic: INSDC center name

text: public

characteristic: SRA accession

text: E-MTAB-5062:Condition #1, Replicate #1

tag: Namespace:Vanderilt University Medical Center Department of Pathology, Microbiology, and Immunology

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: Protocols: Following an initial inoculum of OD600 = 0.01, Helicobacter cinaedi ATCC BAA-847 was propagated at 37°C under the following mono-culture (bacteria) and co-culture (bacteria + epithelial monolayer) conditions: [1] monophasic (liquid alone) F12 medium, H2-supplemented microaerobic atmosphere (n=4); [2] biphasic (liquid over agar) F12 medium, H2-supplemented microaerobic atmosphere (n=4); [3] biphasic F12 medium, H2-free/CO2-supplemented microaerobic atmosphere (n=4); [4] F12 medium over Caco-2 monolayer, H2-supplemented microaerobic atmosphere (n=5); [5] F12 medium over Caco-2 monolayer, CO2-supplemented aerobic atmosphere (n=5); [6] monophasic F12 medium + 10 mM L-lactate, H2-supplemented microaerobic atmosphere (n=4); [7] monophasic F12 medium + 10 mM L-lactate, CO2-supplemented aerobic atmosphere (n=4); [8] monophasic Mueller Hinton broth, H2-supplemented microaerobic atmosphere (n=4); [9] biphasic F12 medium + 10% normal human serum (commercially acquired), H2-supplemented microaerobic atmosphere (n=5). After 24 hours of growth, total RNA was extracted from H. cinaedi specimens. In brief, two volumes of RNA Protect Bacterial Reagent (Qiagen) were added to each culture. Pelleted bacteria were re-treated (10 minutes, 65ºC) with Max Bacterial RNA Enhancement Reagent (Ambion), followed by TRIzol extraction and spin column purification (miRNeasy Mini Kit, Qiagen) of total RNA. Nucleic acid quality was assessed by 260/280 absorbance and 23S/16S rRNA ratios. Illumina library preparation was performed at the Vanderbilt University sequencing core (VANTAGE - Vanderbilt Technologies for Advanced Genomics). From each specimen, ribosomal RNA was depleted with the Gram-Negative Ribo-Zero rRNA Removal Kit (Epicenter). cDNA libraries was constructed for Illumina sequencing through the TruSeq Stranded mRNA Library Prep Kit (Illumina).

characteristic: description

text: H2-supplemented microaerobic

characteristic: environmental stress

text: Monophasic F12 medium

characteristic: growth condition

text: Helicobacter cinaedi CCUG 18818 = ATCC BAA-847

characteristic: organism

text: Condition #1, Replicate #1

type: has member


E-MTAB-5062:Condition #1, Replicate #2

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: Vanderilt University Medical Center Department of Pathology, Microbiology, and Immunology

characteristic: INSDC first public

text: public

text: E-MTAB-5062:Condition #1, Replicate #2

text: ArrayExpress

text: H2-supplemented microaerobic

text: Monophasic F12 medium

text: Condition #1, Replicate #2


E-MTAB-5062:Condition #1, Replicate #3

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-5062:Condition #1, Replicate #3

text: ArrayExpress

text: H2-supplemented microaerobic

text: Monophasic F12 medium

text: Condition #1, Replicate #3


E-MTAB-5062:Condition #1, Replicate #4

text: public

text: E-MTAB-5062:Condition #1, Replicate #4

text: ArrayExpress

text: H2-supplemented microaerobic

text: Monophasic F12 medium

text: Condition #1, Replicate #4

characteristic: title


E-MTAB-5062:Condition #2, Replicate #1

text: public

text: E-MTAB-5062:Condition #2, Replicate #1

text: ArrayExpress

text: H2-supplemented microaerobic

text: Biphasic F12 medium

text: Condition #2, Replicate #1


E-MTAB-5062:Condition #2, Replicate #2

text: public

text: E-MTAB-5062:Condition #2, Replicate #2

text: ArrayExpress

text: H2-supplemented microaerobic

text: Biphasic F12 medium

text: Condition #2, Replicate #2


E-MTAB-5062:Condition #2, Replicate #3

text: public

text: E-MTAB-5062:Condition #2, Replicate #3

text: ArrayExpress

text: H2-supplemented microaerobic

text: Biphasic F12 medium

text: Condition #2, Replicate #3


E-MTAB-5062:Condition #2, Replicate #4

text: public

text: E-MTAB-5062:Condition #2, Replicate #4

text: ArrayExpress

text: H2-supplemented microaerobic

text: Biphasic F12 medium

text: Condition #2, Replicate #4


E-MTAB-5062:Condition #3, Replicate #1

text: public

text: E-MTAB-5062:Condition #3, Replicate #1

text: ArrayExpress

text: H2-free / CO2-supplemented microaerobic

text: Biphasic F12 medium

text: Condition #3, Replicate #1


E-MTAB-5062:Condition #3, Replicate #2

text: public

text: E-MTAB-5062:Condition #3, Replicate #2

text: ArrayExpress

text: H2-free / CO2-supplemented microaerobic

text: Biphasic F12 medium

text: Condition #3, Replicate #2


E-MTAB-5062:Condition #3, Replicate #3

text: public

text: E-MTAB-5062:Condition #3, Replicate #3

text: ArrayExpress

text: H2-free / CO2-supplemented microaerobic

text: Biphasic F12 medium

text: Condition #3, Replicate #3


E-MTAB-5062:Condition #3, Replicate #4

text: public

text: E-MTAB-5062:Condition #3, Replicate #4

text: ArrayExpress

text: H2-free / CO2-supplemented microaerobic

text: Biphasic F12 medium

text: Condition #3, Replicate #4


E-MTAB-5062:Condition #4, Replicate #1

text: public

text: E-MTAB-5062:Condition #4, Replicate #1

text: ArrayExpress

text: H2-supplemented microaerobic

text: F12 medium + Caco-2 cells

text: Condition #4, Replicate #1


E-MTAB-5062:Condition #4, Replicate #2

text: public

text: E-MTAB-5062:Condition #4, Replicate #2

text: ArrayExpress

text: H2-supplemented microaerobic

text: F12 medium + Caco-2 cells

text: Condition #4, Replicate #2


E-MTAB-5062:Condition #4, Replicate #3

text: public

text: E-MTAB-5062:Condition #4, Replicate #3

text: ArrayExpress

text: H2-supplemented microaerobic

text: F12 medium + Caco-2 cells

text: Condition #4, Replicate #3


E-MTAB-5062:Condition #4, Replicate #4

text: public

text: E-MTAB-5062:Condition #4, Replicate #4

text: ArrayExpress

text: H2-supplemented microaerobic

text: F12 medium + Caco-2 cells

text: Condition #4, Replicate #4


E-MTAB-5062:Condition #4, Replicate #5

text: public

text: E-MTAB-5062:Condition #4, Replicate #5

text: ArrayExpress

text: H2-supplemented microaerobic

text: F12 medium + Caco-2 cells

text: Condition #4, Replicate #5


E-MTAB-5062:Condition #5, Replicate #1

text: public

text: E-MTAB-5062:Condition #5, Replicate #1

text: ArrayExpress

text: CO2-supplemented aerobic

text: F12 medium + Caco-2 cells

text: Condition #5, Replicate #1


E-MTAB-5062:Condition #5, Replicate #2

text: public

text: E-MTAB-5062:Condition #5, Replicate #2

text: ArrayExpress

text: CO2-supplemented aerobic

text: F12 medium + Caco-2 cells

text: Condition #5, Replicate #2


E-MTAB-5062:Condition #5, Replicate #3

text: public

text: E-MTAB-5062:Condition #5, Replicate #3

text: ArrayExpress

text: CO2-supplemented aerobic

text: F12 medium + Caco-2 cells

text: Condition #5, Replicate #3


E-MTAB-5062:Condition #5, Replicate #4

text: public

text: E-MTAB-5062:Condition #5, Replicate #4

text: ArrayExpress

text: CO2-supplemented aerobic

text: F12 medium + Caco-2 cells

text: Condition #5, Replicate #4


E-MTAB-5062:Condition #5, Replicate #5

text: public

text: E-MTAB-5062:Condition #5, Replicate #5

text: ArrayExpress

text: CO2-supplemented aerobic

text: F12 medium + Caco-2 cells

text: Condition #5, Replicate #5


E-MTAB-5062:Condition #6, Replicate #1

text: public

text: E-MTAB-5062:Condition #6, Replicate #1

text: ArrayExpress

text: H2-supplemented microaerobic

text: Monophasic F12 medium + 10 mM L-lactate

text: Condition #6, Replicate #1


E-MTAB-5062:Condition #6, Replicate #2

text: public

text: E-MTAB-5062:Condition #6, Replicate #2

text: ArrayExpress

text: H2-supplemented microaerobic

text: Monophasic F12 medium + 10 mM L-lactate

text: Condition #6, Replicate #2


E-MTAB-5062:Condition #6, Replicate #3

text: public

text: E-MTAB-5062:Condition #6, Replicate #3

text: ArrayExpress

text: H2-supplemented microaerobic

text: Monophasic F12 medium + 10 mM L-lactate

text: Condition #6, Replicate #3


E-MTAB-5062:Condition #6, Replicate #4

text: public

text: E-MTAB-5062:Condition #6, Replicate #4

text: ArrayExpress

text: H2-supplemented microaerobic

text: Monophasic F12 medium + 10 mM L-lactate

text: Condition #6, Replicate #4


E-MTAB-5062:Condition #7, Replicate #1

text: public

text: E-MTAB-5062:Condition #7, Replicate #1

text: ArrayExpress

text: CO2-supplemented aerobic

text: Monophasic F12 medium + 10 mM L-lactate

text: Condition #7, Replicate #1


E-MTAB-5062:Condition #7, Replicate #2

text: public

text: E-MTAB-5062:Condition #7, Replicate #2

text: ArrayExpress

text: CO2-supplemented aerobic

text: Monophasic F12 medium + 10 mM L-lactate

text: Condition #7, Replicate #2


E-MTAB-5062:Condition #7, Replicate #3

text: public

text: E-MTAB-5062:Condition #7, Replicate #3

text: ArrayExpress

text: CO2-supplemented aerobic

text: Monophasic F12 medium + 10 mM L-lactate

text: Condition #7, Replicate #3


E-MTAB-5062:Condition #7, Replicate #4

text: public

text: E-MTAB-5062:Condition #7, Replicate #4

text: ArrayExpress

text: CO2-supplemented aerobic

text: Monophasic F12 medium + 10 mM L-lactate

text: Condition #7, Replicate #4


E-MTAB-5062:Condition #8, Replicate #1

text: public

text: E-MTAB-5062:Condition #8, Replicate #1

text: ArrayExpress

text: H2-supplemented microaerobic

text: Monophasic Mueller Hinton Broth

text: Condition #8, Replicate #1


E-MTAB-5062:Condition #8, Replicate #2

text: public

text: E-MTAB-5062:Condition #8, Replicate #2

text: ArrayExpress

text: H2-supplemented microaerobic

text: Monophasic Mueller Hinton Broth

text: Condition #8, Replicate #2


E-MTAB-5062:Condition #8, Replicate #3

text: public

text: E-MTAB-5062:Condition #8, Replicate #3

text: ArrayExpress

text: H2-supplemented microaerobic

text: Monophasic Mueller Hinton Broth

text: Condition #8, Replicate #3


E-MTAB-5062:Condition #8, Replicate #4

text: public

text: E-MTAB-5062:Condition #8, Replicate #4

text: ArrayExpress

text: H2-supplemented microaerobic

text: Monophasic Mueller Hinton Broth

text: Condition #8, Replicate #4


E-MTAB-5062:Condition #9, Replicate #1

text: public

text: E-MTAB-5062:Condition #9, Replicate #1

text: ArrayExpress

text: H2-supplemented microaerobic

text: Monophasic F12 medium + 10% human serum (normal)

text: Condition #9, Replicate #1


E-MTAB-5062:Condition #9, Replicate #2

text: public

text: E-MTAB-5062:Condition #9, Replicate #2

text: ArrayExpress

text: H2-supplemented microaerobic

text: Condition #9, Replicate #2


E-MTAB-5062:Condition #9, Replicate #3

text: public

text: E-MTAB-5062:Condition #9, Replicate #3

text: ArrayExpress

text: H2-supplemented microaerobic

text: Condition #9, Replicate #3


E-MTAB-5062:Condition #9, Replicate #4

text: public

text: E-MTAB-5062:Condition #9, Replicate #4

text: ArrayExpress

text: H2-supplemented microaerobic

text: Condition #9, Replicate #4


E-MTAB-5062:Condition #9, Replicate #5

text: public

text: E-MTAB-5062:Condition #9, Replicate #5

text: ArrayExpress

text: H2-supplemented microaerobic

text: Condition #9, Replicate #5


text: Plasmodium falciparum

characteristic: ArrayExpress-Species

text: SC

text: public

text: malaria parasite P. falciparum

characteristic: common name

text: Plasmodium falciparum

text: Plasmodium falciparum


text: mESC

characteristic: ArrayExpress-CellType

text: female

characteristic: ArrayExpress-Sex

text: Mus musculus

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: SC

text: public

text: house mouse

text: Mus musculus

text: RNAseq libraries from rRNA depleted whole RNA

characteristic: sample description

text: Mus musculus


text: testis

characteristic: ArrayExpress-OrganismPart

text: Mus musculus

text: SC

characteristic: INSDC center name

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: house mouse

text: Mus musculus

text: Illumina TruSeq Small RNA library

text: Mus musculus


text: synthetic construct

characteristic: ENA-CHECKLIST

text: SC

text: public

text: synthetic construct

text: synthetic construct

characteristic: organism

text: synthetic construct


text: neurons

text: IP

characteristic: ArrayExpress-Immunoprecipitate

text: female

text: Homo sapiens

characteristic: ENA-LAST-UPDATE

text: SC

characteristic: INSDC first public

text: public

text: human

text: Homo sapiens

text: ChIPmentation

text: Homo sapiens

text: IP KO H4 TG 1


text: neurons

text: IP

text: female

text: Homo sapiens

text: SC

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: human

text: Homo sapiens

text: ChIPmentation

text: Homo sapiens

text: IP WT TG 1

characteristic: title


text: neurons

characteristic: ArrayExpress-CellType

text: IP

text: female

text: Homo sapiens

characteristic: ArrayExpress-Species

text: SC

text: public

text: human

characteristic: common name

text: Homo sapiens

text: ChIPmentation

text: Homo sapiens

text: IP 35 E4 TG 1


text: neurons

text: IP

text: female

characteristic: ArrayExpress-Sex

text: Homo sapiens

characteristic: ENA-FIRST-PUBLIC

characteristic: External Id

text: SC

text: public

text: human

text: Homo sapiens

text: ChIPmentation

text: Homo sapiens

text: IP 33 C10 TG 1


text: neurons

text: input

text: female

text: Homo sapiens

text: SC

characteristic: INSDC center name

text: public

text: human

text: Homo sapiens

text: Input tagmented

characteristic: sample description

text: Homo sapiens

text: Input KO H4 TG 1


text: neurons

text: input

text: female

text: Homo sapiens

text: SC

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: human

text: Homo sapiens

text: Input tagmented

text: Homo sapiens

text: Input WT TG 1


text: neurons

text: input

text: female

text: Homo sapiens

characteristic: ENA-CHECKLIST

text: SC

text: public

text: human

text: Homo sapiens

characteristic: organism

text: Input tagmented

text: Homo sapiens

text: Input 35 E4 TG 1


text: neurons

text: input

characteristic: ArrayExpress-Immunoprecipitate

text: female

text: Homo sapiens

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: human

text: Homo sapiens

text: Input tagmented

text: Homo sapiens

text: Input 33 C10 TG 1


text: Staphylococcus aureus

text: SC

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: Staphylococcus aureus

text: Staphylococcus aureus

text: Staphylococcus aureus


text: WT

characteristic: ArrayExpress-Phenotype

text: Neisseria meningitidis

text: SC

text: public

characteristic: INSDC status

text: Neisseria meningitidis

text: Neisseria meningitidis

text: Neisseria meningitidis

characteristic: title


text: Homo sapiens

characteristic: ArrayExpress-Species

text: SC

text: public

text: human

characteristic: common name

text: Homo sapiens

text: Preterm infant faeces

text: Homo sapiens


text: Anopheles gambiae

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: African malaria mosquito

text: Anopheles gambiae

text: Anopheles gambiae


E-MTAB-4929:AC Colombe 1

characteristic: External Id

text: University of Calgary

text: public

text: E-MTAB-4929:AC Colombe 1

tag: Namespace:University of Calgary

text: ArrayExpress

characteristic: broker name

text: soybean

text: AC Colombe

characteristic: cultivar

text: Protocols: Two week-old soybean roots were harvested, flash-frozen with liquid nitrogen and ground to a fine powder Planted in Pro-Mix BX MycorrhizaeTM soil (Rivière-du-Loup, Canada) in a growth chamber with a 16 h light cycle at 25°C, and an 8 h dark cycle at 20°C, with 60-70% relative humidity, with light intensity 100-150 μmol m-2s-1. RNA was extracted from the twelve samples according to the instructions in the RNeasy Mini Kit (Qiagen, Germany). RNA integrity and concentration were recorded using the Agilent 2100 (Bioanalyzer, USA). A total of 4 µg of RNA, at a concentration of approximately 100 ng/µL was used per replicate Library constructed from soybean root RNA

characteristic: description

unit: concentration unit

characteristic: metabolic process

text: Glycine max

text: Glycine max

text: root

characteristic: organism part

text: High Isoflavonoid

characteristic: phenotype

text: Glycine max

text: AC Colombe 1


E-MTAB-4929:AC Colombe 2

characteristic: ENA-CHECKLIST

text: University of Calgary

characteristic: INSDC center name

text: public

characteristic: SRA accession

text: E-MTAB-4929:AC Colombe 2

characteristic: Submitter Id

text: ArrayExpress

text: soybean

text: AC Colombe

text: Glycine max

characteristic: organism

text: Glycine max

text: root

text: High Isoflavonoid

text: Glycine max

text: AC Colombe 2


E-MTAB-4929:AC Colombe 3

characteristic: ENA-LAST-UPDATE

text: University of Calgary

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-4929:AC Colombe 3

text: ArrayExpress

text: soybean

text: AC Colombe

text: Glycine max

text: Glycine max

text: root

text: High Isoflavonoid

text: Glycine max

text: AC Colombe 3


E-MTAB-4929:AC Glengarry 1

characteristic: ENA-FIRST-PUBLIC

text: University of Calgary

text: public

text: E-MTAB-4929:AC Glengarry 1

text: ArrayExpress

text: soybean

characteristic: common name

text: AC Glengarry

text: Glycine max

text: Glycine max

text: root

text: Low Isoflavonoid

text: Glycine max

text: AC Glengarry 1

characteristic: title


E-MTAB-4929:AC Glengarry 2

characteristic: External Id

text: University of Calgary

text: public

text: E-MTAB-4929:AC Glengarry 2

text: ArrayExpress

characteristic: broker name

text: soybean

text: AC Glengarry

characteristic: cultivar

text: Glycine max

text: Glycine max

text: root

text: Low Isoflavonoid

text: Glycine max

text: AC Glengarry 2


E-MTAB-4929:AC Glengarry 3

text: University of Calgary

text: public

text: E-MTAB-4929:AC Glengarry 3

text: ArrayExpress

text: soybean

text: AC Glengarry

characteristic: description

characteristic: metabolic process

text: Glycine max

text: Glycine max

text: root

characteristic: organism part

text: Low Isoflavonoid

text: Glycine max

text: AC Glengarry 3


E-MTAB-4929:Conrad 1

characteristic: ENA-CHECKLIST

text: University of Calgary

characteristic: INSDC center name

text: public

text: E-MTAB-4929:Conrad 1

text: ArrayExpress

text: soybean

text: Conrad

text: Glycine max

text: Glycine max

text: root

text: High Isoflavonoid

characteristic: phenotype

text: Glycine max

text: Conrad 1


E-MTAB-4929:Conrad 2

text: University of Calgary

text: public

characteristic: SRA accession

text: E-MTAB-4929:Conrad 2

characteristic: Submitter Id

text: ArrayExpress

text: soybean

text: Conrad

text: Glycine max

characteristic: organism

text: Glycine max

text: root

text: High Isoflavonoid

text: Glycine max

text: Conrad 2


E-MTAB-4929:Conrad 3

characteristic: ENA-LAST-UPDATE

text: University of Calgary

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-4929:Conrad 3

text: ArrayExpress

text: soybean

text: Conrad

text: Glycine max

text: Glycine max

text: root

text: High Isoflavonoid

text: Glycine max

text: Conrad 3


E-MTAB-4929:Pagoda 1

characteristic: ENA-FIRST-PUBLIC

text: University of Calgary

text: public

characteristic: INSDC status

text: E-MTAB-4929:Pagoda 1

text: ArrayExpress

text: soybean

characteristic: common name

text: Pagoda

text: Glycine max

text: Glycine max

text: root

text: Low Isoflavonoid

text: Glycine max

text: Pagoda 1


E-MTAB-4929:Pagoda 2

characteristic: External Id

text: University of Calgary

text: public

text: E-MTAB-4929:Pagoda 2

text: ArrayExpress

characteristic: broker name

text: soybean

text: Pagoda

text: Glycine max

text: Glycine max

text: root

text: Low Isoflavonoid

text: Glycine max

text: Pagoda 2

characteristic: title


E-MTAB-4929:Pagoda 3

text: University of Calgary

text: public

text: E-MTAB-4929:Pagoda 3

text: ArrayExpress

text: soybean

text: Pagoda

characteristic: cultivar

characteristic: description

characteristic: metabolic process

text: Glycine max

text: Glycine max

text: root

text: Low Isoflavonoid

text: Glycine max

text: Pagoda 3


characteristic: ENA-CHECKLIST

text: University of Sao Paulo

characteristic: INSDC center name

text: public

tag: Namespace:University of Sao Paulo

text: animal 1

text: Bos indicus

text: Bos indicus


text: University of Sao Paulo

text: public

characteristic: SRA accession

text: animal 2

text: Bos indicus

text: Bos indicus


text: University of Sao Paulo

text: public

characteristic: Submitter Id

text: animal 3

text: Bos indicus

text: Bos indicus


text: University of Sao Paulo

text: public

text: animal 4

text: Bos indicus

characteristic: organism

text: Bos indicus


characteristic: ENA-LAST-UPDATE

text: University of Sao Paulo

text: public

text: animal 5

text: Bos indicus

text: Bos indicus


text: University of Sao Paulo

characteristic: INSDC first public

text: public

text: animal 6

text: Bos indicus

text: Bos indicus


text: University of Sao Paulo

characteristic: INSDC last update

text: public

text: animal 7

text: Bos indicus

text: Bos indicus


characteristic: ENA-FIRST-PUBLIC

text: University of Sao Paulo

text: public

text: animal 8

text: Bos indicus

text: Bos indicus


text: University of Sao Paulo

text: public

characteristic: INSDC status

text: animal 9

text: Bos indicus

text: Bos indicus


text: University of Sao Paulo

text: public

text: animal 10

text: Bos indicus

text: Bos indicus


text: University of Sao Paulo

text: public

text: animal 11

text: Bos indicus

text: Bos indicus


text: University of Sao Paulo

text: public

text: animal 12

text: Bos indicus

text: Bos indicus


text: University of Sao Paulo

text: public

text: animal 13

text: Bos indicus

text: Bos indicus


text: University of Sao Paulo

text: public

text: animal 14

text: Bos indicus

text: Bos indicus


text: University of Sao Paulo

text: public

text: animal 15

text: Bos indicus

text: Bos indicus


text: University of Sao Paulo

text: public

text: animal 16

text: Bos indicus

text: Bos indicus


text: University of Sao Paulo

text: public

text: animal 17

text: Bos indicus

text: Bos indicus


text: University of Sao Paulo

text: public

text: animal 18

text: Bos indicus

text: Bos indicus


text: University of Sao Paulo

text: public

text: animal 19

text: Bos indicus

text: Bos indicus


text: University of Sao Paulo

text: public

text: animal 20

text: Bos indicus

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J.0126 cp complete gneome

text: public

text: J.0126 cp complete gneome

tag: Namespace:School of BioSciences, The University of Melbourne

text: J.0126 cp Liagora brachyclada complete gneome

text: Liagora brachyclada

text: Liagora brachyclada

characteristic: specimen voucher

text: J.0126 cp complete gneome


characteristic: External Id

text: public

tag: Namespace:Institute of Biotechnology, Univerity of Helsinki

text: Comparative genomics of Propionibacterium freudenrechii, a GRAS organism with industrial applications and probiotic potential

text: Propionibacterium freudenreichii

characteristic: host

text: Propionibacterium freudenreichii

text: Propionibacterium freudenreichii


text: public

text: fruit fly

characteristic: common name

text: Comparison between ancestral populations and reconstituted ancestral populations

text: Drosophila melanogaster

text: Drosophila melanogaster

text: AncPopRec

characteristic: title


text: public

characteristic: description

text: Drosophila simulans

text: Drosophila simulans

text: AncPopRec


text: NETHERLANDS INSTITUTE FOR ECOLOGY

text: public

tag: Namespace:NETHERLANDS INSTITUTE FOR ECOLOGY

characteristic: collection date

text: Fungal amplicons from a DNA-SIP study on grassland soils

text: Grassland

characteristic: environment (biome)

text: soil

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: Netherlands

characteristic: geographic location (country and/or sea)

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: mimarks-survey

characteristic: investigation type

text: metagenome

text: plant-associated

characteristic: plant-associated environmental package

text: Ecofinders Fungi DNA-SIP

characteristic: project name

text: metagenome

characteristic: sequencing method


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: NETHERLANDS INSTITUTE FOR ECOLOGY

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Fungal amplicons from a DNA-SIP study on grassland soils, Soil OR, 13C fraction

text: Grassland

text: soil

text: soil

text: Netherlands

text: metagenome

text: metagenome

characteristic: organism

text: plant-associated

text: Ecofinders Fungi DNA-SIP

text: metagenome


characteristic: External Id

text: NETHERLANDS INSTITUTE FOR ECOLOGY

text: public

characteristic: collection date

characteristic: description

text: Grassland

characteristic: environment (biome)

text: soil

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: Netherlands

characteristic: geographic location (country and/or sea)

text: metagenome

text: metagenome

text: plant-associated

text: Ecofinders Fungi DNA-SIP

text: metagenome

characteristic: title


unit: mg Chl/m3

characteristic: Chlorophyll Sensor

text: Tara Oceans Consortium, Coordinators & Tara Oceans Expedition, Participants. Registry of all samples from the Tara Oceans Expedition (2009-2013). PANGAEA. doi:10.1594/PANGAEA.842197 (2015) (https://dx.doi.org/10.1594/PANGAEA.842197)

characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: Event Date/Time End

text: GSC

text: public

characteristic: Latitude End

characteristic: Longitude End

unit: practical salinity unit

characteristic: Salinity Sensor

unit: m

characteristic: depth

text: This sample (TARA_B110000003) was collected at station TARA_155 during campaign TARA_20130519Z of the Tara Oceans expedition (2009-2013), using a high volume peristaltic pump [HVP-PUMP]. The sampling event (TARA_20130524T0536Z_155_EVENT_PUMP) occurred at position latitudeN=54.5305 and longitudeE=-16.9377, on date/time=2013-05-24T05:36:00, at a depth of 3-7 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated (0.22-3 micrometres) and prepared on board using protocol [SEQ-(100L)_W0.22-3] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes. This sample has replicate sample(s): TARA_B110000002.

text: Westerlies Biome

text: discrete depth within the epipelagic zone (ENVO:00000209) at the top of the photic zone (ENVO:00000209), <50 m from a strong peak in chlorophyll fluorescence

characteristic: environment (feature) further details

text: water (ENVO:00002006), including particulate matter (ENVO:01000060) such as planktonic material (ENVO:01000063)

text: water

characteristic: environmental package

characteristic: event date/time start

characteristic: event label

characteristic: further details

characteristic: last update date

characteristic: latitude start

characteristic: longitude start

text: [NAO] North Atlantic Ocean (MRGID:1912)

characteristic: marine region

unit: µmol/L

characteristic: nitrate sensor

text: marine metagenome

unit: µmol/kg

characteristic: oxygen sensor

text: Tara Oceans Expedition (2009-2013)

characteristic: project name

text: In the size fraction W0.22-3 micrometres, the sample material was pre-filtered on 3-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane (ref:TSTP14250), and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 100 Litres was filtered and the 0.22-micrometre-pore-size filter was packaged in a sterile Falcon tube (50 mL), treated with the addition of 10 mL of RNA-Later, labelled with a barcode identification sticker and stored on board in a freezer at -20 degree Celsius. Note 1: Latex or nitril gloves were used for this protocol. Note 2: All containers, filter holders and tubing were washed with 0.1% bleach, rinsed twice with fresh water and rinsed again twice with the pre-filtered sample material. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump (Masterflex, EW-77410-10) was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. A total of 2 replicates were prepared and sent for later analysis to the Institute of Marine Science (CSIC), Barcelona, Spain.

characteristic: protocol description

characteristic: protocol label

text: high volume peristaltic pump [HVP-PUMP]

characteristic: sample collection device

characteristic: sample material label

text: This version can be used to provide data discovery services

characteristic: sample status

characteristic: sampling campaign

text: SV Tara

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

unit: ºC

characteristic: temperature

text: TARA_20130524T0536Z_155_EVENT_PUMP_SRF_(5m)_SEQ-(100L)_W0.22-3_B110000003


characteristic: Chlorophyll Sensor

characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: GSC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (TARA_N010000582) was collected at station TARA_155 during campaign TARA_20130519Z of the Tara Oceans expedition (2009-2013), using a high volume peristaltic pump [HVP-PUMP]. The sampling event (TARA_20130524T0536Z_155_EVENT_PUMP) occurred at position latitudeN=54.5305 and longitudeE=-16.9377, on date/time=2013-05-24T05:36:00, at a depth of 3-7 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated (>0.8 micrometres) and prepared on board using protocol [SEQ-(100L-or-15min)_W>0.8] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity, metagenomics and/or transcriptomics studies of unicellular eukaryotes (protists). This sample has replicate sample(s): TARA_N010000580, TARA_N010000581, TARA_N010000583.

characteristic: description

text: Westerlies Biome

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (feature) further details

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

characteristic: event label

characteristic: further details

characteristic: last update date

characteristic: latitude start

text: [NAO] North Atlantic Ocean (MRGID:1912)

text: marine metagenome

characteristic: organism

text: Tara Oceans Expedition (2009-2013)

text: In the size fraction W>0.8 micrometres, the sample material was concentrated on 0.8-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane filter (ref:ATTP14250) on top of a Dacron pad mesh. A volume of 100 Litres was filtered in parallel on two 0.8-micrometre-pore-size filters. Each filter was then packaged in a sterile cryogenic tube (5 mL) (2 tubes, each with 1 filter), labelled with a barcode identification sticker and stored on board in liquid nitrogen. Note 1: Latex or nitril gloves were used for this protocol. Note 2: All containers, filter holders and tubing were washed with 0.1% bleach, rinsed twice with fresh water and rinsed again twice with the pre-filtered sample material. All tweezers are kept clean with ethanol. Note 3: The entire process did not exceed 15 minutes. Note 4: The total volume filtered on the two filters is 100 Litres. However, the volume filtered on each filter is unknown, but approximately 50 Litres. The intention of this protocol is to combine the two filters during molecular entities (nucleotides) extraction and sequencing analysis. A total of 2 (2x 2 tubes) replicates were prepared and sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

text: high volume peristaltic pump [HVP-PUMP]

text: SV Tara

text: marine metagenome

text: TARA_20130524T0536Z_155_EVENT_PUMP_SRF_(5m)_SEQ-(100L-or-15min)_W>0.8_N010000582

characteristic: title


characteristic: Chlorophyll Sensor

characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: GSC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

text: This sample (TARA_N010000586) was collected at station TARA_155 during campaign TARA_20130519Z of the Tara Oceans expedition (2009-2013), using a high volume peristaltic pump [HVP-PUMP]. The sampling event (TARA_20130524T0536Z_155_EVENT_PUMP) occurred at position latitudeN=54.5305 and longitudeE=-16.9377, on date/time=2013-05-24T05:36:00, at a depth of 3-7 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated (3-20 micrometres) and prepared on board using protocol [SEQ-(10-100L-or-15min)_W3-20] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity, metagenomics and/or transcriptomics studies of unicellular eukaryotes (protists) and attached prokaryotes. This sample has replicate sample(s): TARA_N010000587, TARA_N010000588, TARA_N010000585, TARA_N010000584.

text: Westerlies Biome

text: water

characteristic: longitude start

text: [NAO] North Atlantic Ocean (MRGID:1912)

characteristic: marine region

characteristic: nitrate sensor

text: marine metagenome

characteristic: oxygen sensor

text: Tara Oceans Expedition (2009-2013)

characteristic: project name

text: In the size fraction W3-20 micrometres, the sample material was pre-filtered on 20-micrometres, using a nylon mesh, and concentrated on 3-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane filter (ref:TSTP14250). A volume of 10-100 Litres was filtered and the 3-micrometre-pore-size filter was packaged in a sterile cryogenic tube (5 mL), labelled with a barcode identification sticker and stored on board in liquid nitrogen. Note 1: Latex or nitril gloves were used for this protocol. Note 2: All containers, filter holders and tubing were washed with 0.1% bleach, rinsed twice with fresh water and rinsed again twice with the pre-filtered sample material. All tweezers are kept clean with ethanol. Note 3: The entire process did not exceed 15 minutes. Note 4: The volume filtered is indicated for each replicate in the Sample registry (http://doi.pangaea.de/10.1594/PANGAEA.842197). A total of 2-6 replicates were prepared and sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

characteristic: protocol description

characteristic: protocol label

text: high volume peristaltic pump [HVP-PUMP]

characteristic: sample collection device

characteristic: sample material label

characteristic: sample status

characteristic: sampling campaign

text: SV Tara

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: TARA_20130524T0536Z_155_EVENT_PUMP_SRF_(5m)_SEQ-(10-100L-or-15min)_W3-20_N010000586


characteristic: Chlorophyll Sensor

characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: GSC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (TARA_N010000588) was collected at station TARA_155 during campaign TARA_20130519Z of the Tara Oceans expedition (2009-2013), using a high volume peristaltic pump [HVP-PUMP]. The sampling event (TARA_20130524T0536Z_155_EVENT_PUMP) occurred at position latitudeN=54.5305 and longitudeE=-16.9377, on date/time=2013-05-24T05:36:00, at a depth of 3-7 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated (3-20 micrometres) and prepared on board using protocol [SEQ-(10-100L-or-15min)_W3-20] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity, metagenomics and/or transcriptomics studies of unicellular eukaryotes (protists) and attached prokaryotes. This sample has replicate sample(s): TARA_N010000584, TARA_N010000585, TARA_N010000586, TARA_N010000587.

characteristic: description

text: Westerlies Biome

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (feature) further details

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

characteristic: event label

characteristic: further details

characteristic: last update date

characteristic: latitude start

text: [NAO] North Atlantic Ocean (MRGID:1912)

text: marine metagenome

characteristic: organism

text: Tara Oceans Expedition (2009-2013)

text: high volume peristaltic pump [HVP-PUMP]

text: SV Tara

text: marine metagenome

text: TARA_20130524T0536Z_155_EVENT_PUMP_SRF_(5m)_SEQ-(10-100L-or-15min)_W3-20_N010000588

characteristic: title


characteristic: Chlorophyll Sensor

characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: GSC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

text: This sample (TARA_B110001435) was collected at station TARA_155 during campaign TARA_20130519Z of the Tara Oceans expedition (2009-2013), using a high volume peristaltic pump [HVP-PUMP]. The sampling event (TARA_20130524T0536Z_155_EVENT_PUMP) occurred at position latitudeN=54.5305 and longitudeE=-16.9377, on date/time=2013-05-24T05:36:00, at a depth of 3-7 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated (0.22-3 micrometres) and prepared on board using protocol [SEQ-(10L-or-15min)_W0.22-3] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity, metagenomics and/or transcriptomics studies of prokaryotes. This sample has replicate sample(s): TARA_B110001436.

text: Westerlies Biome

text: water

characteristic: longitude start

text: [NAO] North Atlantic Ocean (MRGID:1912)

characteristic: marine region

characteristic: nitrate sensor

text: marine metagenome

characteristic: oxygen sensor

text: Tara Oceans Expedition (2009-2013)

characteristic: project name

text: In the size fraction W0.22-3 micrometres, the sample material was pre-filtered on 3-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane (ref:TSTP14250), and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 10 Litres was filtered and the 0.22-micrometre-pore-size filter was packaged in a sterile Falcon tube (50 mL), treated with the addition of 10 mL of RNA-Later, labelled with a barcode identification sticker and stored on board in a freezer at -20 degree Celsius. Note 1: Latex or nitril gloves were used for this protocol. Note 2: All containers, filter holders and tubing were washed with 0.1% bleach, rinsed twice with fresh water and rinsed again twice with the pre-filtered sample material. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump (Masterflex, EW-77410-10) was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The entire process did not exceed 15 minutes. A total of 2 replicates were prepared and sent for later analysis to the Institute of Marine Science (CSIC), Barcelona, Spain.

characteristic: protocol description

characteristic: protocol label

text: high volume peristaltic pump [HVP-PUMP]

characteristic: sample collection device

characteristic: sample material label

characteristic: sample status

characteristic: sampling campaign

text: SV Tara

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: TARA_20130524T0536Z_155_EVENT_PUMP_SRF_(5m)_SEQ-(10L-or-15min)_W0.22-3_B110001435


characteristic: Chlorophyll Sensor

text: Tara Oceans Consortium, Coordinators & Tara Oceans Expedition, Participants. Registry of all samples from the Tara Oceans Expedition (2009-2013). PANGAEA. doi:10.1594/PANGAEA.842197 (2015) (https://dx.doi.org/10.1594/PANGAEA.842197)

characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: GSC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (TARA_R110002003) was collected at station TARA_155 during campaign TARA_20130519Z of the Tara Oceans expedition (2009-2013), using a high volume peristaltic pump [HVP-PUMP]. The sampling event (TARA_20130524T0536Z_155_EVENT_PUMP) occurred at position latitudeN=54.5305 and longitudeE=-16.9377, on date/time=2013-05-24T05:36:00, at a depth of 3-7 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated (<0.22 micrometres) and prepared on board using protocol [SEQ-(FeCl3)_W<0.22] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of viruses. This sample has no known replicate.

characteristic: description

text: Westerlies Biome

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (feature) further details

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

characteristic: event label

characteristic: further details

characteristic: last update date

characteristic: latitude start

text: [NAO] North Atlantic Ocean (MRGID:1912)

text: marine metagenome

characteristic: organism

text: Tara Oceans Expedition (2009-2013)

text: In the size fraction W<0.22 micrometres, the sample material was pre-filtered using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane (ref:GPWP14250) with 0.22-micrometres-pore-size. A volume of 20 Litres was treated with FeCl3, incubated at room temperature for 1-24 hours to allow viruses to precipitate, filtered onto a 1-micrometre-pore-size, 142-mm-diameter GE polycarbonate (PC) membrane filter (ref:K10CP14220) placed on top of a 0.8-micrometre-pore-size, 142-mm-diameter Pall Gelman support filter (ref:60114) using a peristaltic pump with pressure <15 psi. The 1-micrometre-pore-size filter was then packaged in a sterile Falcon tube (50 mL) (up to 3 filters in 1 tube), labelled with a barcode identification sticker and stored on board in a refrigerator at +4 degree Celsius. Note 1: Latex or nitril gloves were used for this protocol. Note 2: All containers, filter holders and tubing were washed with 0.1% bleach, rinsed twice with fresh water and rinsed again twice with the pre-filtered sample material. All tweezers are kept clean with ethanol. Note 3: The three replicates were incubated together. For 20 Litres of filtrate, 2 millilitres of FeCl3 stock solution (4.83 grams of FeCl3-6H2O + 100 millilitres of MilliQ water, stored at 4°C) was added (final concentration of 1 mg Fe per Litre of filtrate), and shaken vigorously for 1 minute. Note 4: Further details about the method are published here doi:10.1111/j.1758-2229.2010.00208.x. In 2009-2012, a total of 3 replicates were prepared and sent for later analysis to the University of Arizona (UA), Tucson, USA. In 2013, a total of 2 replicates were prepared, one sent to for later analysis to the University of Arizona (UA), Tucson, USA, and the other sent for later analysis to the Observatoire Océanologique de Banyuls sur Mer (OOB), France.

text: high volume peristaltic pump [HVP-PUMP]

text: SV Tara

text: marine metagenome

text: TARA_20130524T0536Z_155_EVENT_PUMP_SRF_(5m)_SEQ-(FeCl3)_W<0.22_R110002003

characteristic: title


characteristic: Chlorophyll Sensor

characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: GSC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

text: This sample (TARA_N010000614) was collected at station TARA_155 during campaign TARA_20130519Z of the Tara Oceans expedition (2009-2013), using a plankton net [NET-BONGO-180]+[SIEVE-2000]. The sampling event (TARA_20130524T0754Z_155_EVENT_NET) occurred at position latitudeN=54.5651 and longitudeE=-16.8346, on date/time=2013-05-24T07:54:00, at a depth of 3-7 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated (180-2000 micrometres) and prepared on board using protocol [SEQ-(500mL-or-15min)_N180-2000] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity, metagenomics and/or transcriptomics studies of unicellular eukaryotes (protists). This sample has replicate sample(s): TARA_N010000594, TARA_N010000595, TARA_N010000615.

text: Westerlies Biome

text: discrete depth within the epipelagic zone (ENVO:00000209) at the top of the photic zone (ENVO:00000209), <10 m from a strong peak in chlorophyll fluorescence

text: particulate matter (ENVO:01000060), including planktonic material (ENVO:01000063)

text: water

characteristic: longitude start

text: [NAO] North Atlantic Ocean (MRGID:1912)

characteristic: marine region

characteristic: nitrate sensor

text: marine metagenome

characteristic: oxygen sensor

text: Tara Oceans Expedition (2009-2013)

characteristic: project name

text: In the size fraction N180-2000 micrometres, the sample material was obtained from a net-cod-end collector lined with a nylon mesh of 180-micrometres and passed through a sieve with a metal mesh of 2000-micrometres. Filtered sea water (<0.1-micrometre) was added to the filtrate up to a total volume of 3 Litres. A volume of 500 mL from the diluted filtrate was filtered in parallel on two 10-micrometres-pore-size, 47-mm-diameter Millipore polycarbonate membrane filters (ref:TCTP04700), packaged in a sterile cryogenic tube (5 mL) (2 filters in 1 tube), labelled with a barcode identification sticker and stored on board in liquid nitrogen. Note 1: Latex or nitril gloves were used for this protocol. Note 2: All containers, filter holders and tubing were washed with 0.1% bleach, rinsed twice with fresh water and rinsed again twice with the pre-filtered sample material. All tweezers are kept clean with ethanol. Note 3: The entire process did not exceed 15 minutes. A total of 2 replicates were prepared and sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

characteristic: protocol description

characteristic: protocol label

characteristic: sample collection device

characteristic: sample material label

text: This version can be used to provide data discovery services

characteristic: sample status

characteristic: sampling campaign

text: SV Tara

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: TARA_20130524T0754Z_155_EVENT_NET_SRF_(5m)_SEQ-(500mL-or-15min)_N180-2000_N010000614


characteristic: Chlorophyll Sensor

characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: GSC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (TARA_N010000616) was collected at station TARA_155 during campaign TARA_20130519Z of the Tara Oceans expedition (2009-2013), using a plankton net [NET-DOUBLE-20]+[SIEVE-180]. The sampling event (TARA_20130524T1255Z_155_EVENT_NET) occurred at position latitudeN=54.5636 and longitudeE=-16.8471, on date/time=2013-05-24T12:55:00, at a depth of 3-7 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated (20-180 micrometres) and prepared on board using protocol [SEQ-(500mL-or-15min)_N20-180] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity, metagenomics and/or transcriptomics studies of unicellular eukaryotes (protists). This sample has replicate sample(s): TARA_N010000598, TARA_N010000599, TARA_N010000617.

characteristic: description

text: Westerlies Biome

characteristic: environment (biome)

characteristic: environment (feature)

text: discrete depth within the epipelagic zone (ENVO:00000209) at the top of the photic zone (ENVO:00000209), <50 m from a strong peak in chlorophyll fluorescence

characteristic: environment (feature) further details

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

characteristic: event label

characteristic: further details

characteristic: last update date

characteristic: latitude start

text: [NAO] North Atlantic Ocean (MRGID:1912)

text: marine metagenome

characteristic: organism

text: Tara Oceans Expedition (2009-2013)

text: In the size fraction N20-180 micrometres, the sample material was obtained from a net-cod-end collector lined with a nylon mesh of 20-micrometres and passed through a sieve with a metal mesh of 180-micrometres. Filtered sea water (<0.1-micrometre) was added to the filtrate up to a total volume of 3 Litres. A volume of 500 mL from the diluted filtrate was filtered in parallel on two 10-micrometres-pore-size, 47-mm-diameter Millipore polycarbonate membrane filters (ref:TCTP04700), packaged in a sterile cryogenic tube (5 mL) (2 filters in 1 tube), labelled with a barcode identification sticker and stored on board in liquid nitrogen. Note 1: Latex or nitril gloves were used for this protocol. Note 2: All containers, filter holders and tubing were washed with 0.1% bleach, rinsed twice with fresh water and rinsed again twice with the pre-filtered sample material. All tweezers are kept clean with ethanol. Note 3: The entire process did not exceed 15 minutes. A total of 2 replicates were prepared and sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

text: SV Tara

text: marine metagenome

text: TARA_20130524T1255Z_155_EVENT_NET_SRF_(5m)_SEQ-(500mL-or-15min)_N20-180_N010000616

characteristic: title


characteristic: Chlorophyll Sensor

characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: GSC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

text: This sample (TARA_B110000014) was collected at station TARA_155 during campaign TARA_20130519Z of the Tara Oceans expedition (2009-2013), using a Rosette Vertical Sampling System [RVSS]. The sampling event (TARA_20130524T1315Z_155_Combined-EVENTS_CAST) occurred at position latitudeN=54.5742 and longitudeE=-16.8345, on date/time=2013-05-24T13:15:00, at a depth of 742-760 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [MES] marine water layer (ENVO:01000295) within the mesopelagic zone (ENVO:00000213). The sample material was size-fractionated (0.22-3 micrometres) and prepared on board using protocol [SEQ-(100L)_W0.22-3] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes. This sample has replicate sample(s): TARA_B110000015.

text: Westerlies Biome

text: discrete depth between 400-800 m, within the mesopelagic zone (ENVO:00000213) within the aphotic zone (ENVO:00000210)

text: water (ENVO:00002006), including particulate matter (ENVO:01000060) such as planktonic material (ENVO:01000063)

text: water

characteristic: longitude start

text: [NAO] North Atlantic Ocean (MRGID:1912)

characteristic: marine region

characteristic: nitrate sensor

text: marine metagenome

characteristic: oxygen sensor

text: Tara Oceans Expedition (2009-2013)

characteristic: project name

text: In the size fraction W0.22-3 micrometres, the sample material was pre-filtered on 3-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane (ref:TSTP14250), and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 100 Litres was filtered and the 0.22-micrometre-pore-size filter was packaged in a sterile Falcon tube (50 mL), treated with the addition of 10 mL of RNA-Later, labelled with a barcode identification sticker and stored on board in a freezer at -20 degree Celsius. Note 1: Latex or nitril gloves were used for this protocol. Note 2: All containers, filter holders and tubing were washed with 0.1% bleach, rinsed twice with fresh water and rinsed again twice with the pre-filtered sample material. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump (Masterflex, EW-77410-10) was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. A total of 2 replicates were prepared and sent for later analysis to the Institute of Marine Science (CSIC), Barcelona, Spain.

characteristic: protocol description

characteristic: protocol label

text: Rosette Vertical Sampling System [RVSS]

characteristic: sample collection device

characteristic: sample material label

characteristic: sample status

characteristic: sampling campaign

text: SV Tara

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: TARA_20130524T1315Z_155_Combined-EVENTS_CAST_MES_(751m)_SEQ-(100L)_W0.22-3_B110000014


characteristic: Chlorophyll Sensor

text: Tara Oceans Consortium, Coordinators & Tara Oceans Expedition, Participants. Registry of all samples from the Tara Oceans Expedition (2009-2013). PANGAEA. doi:10.1594/PANGAEA.842197 (2015) (https://dx.doi.org/10.1594/PANGAEA.842197)

characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: GSC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (TARA_N010000610) was collected at station TARA_155 during campaign TARA_20130519Z of the Tara Oceans expedition (2009-2013), using a Rosette Vertical Sampling System [RVSS]. The sampling event (TARA_20130524T1315Z_155_Combined-EVENTS_CAST) occurred at position latitudeN=54.5742 and longitudeE=-16.8345, on date/time=2013-05-24T13:15:00, at a depth of 742-760 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [MES] marine water layer (ENVO:01000295) within the mesopelagic zone (ENVO:00000213). The sample material was size-fractionated (>0.8 micrometres) and prepared on board using protocol [SEQ-(100L-or-15min)_W>0.8] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity, metagenomics and/or transcriptomics studies of unicellular eukaryotes (protists). This sample has replicate sample(s): TARA_N010000609, TARA_N010000608, TARA_N010000611, TARA_N010000612, TARA_N010000613.

characteristic: description

text: Westerlies Biome

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (feature) further details

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

characteristic: event label

characteristic: further details

characteristic: last update date

characteristic: latitude start

text: [NAO] North Atlantic Ocean (MRGID:1912)

text: marine metagenome

characteristic: organism

text: Tara Oceans Expedition (2009-2013)

text: In the size fraction W>0.8 micrometres, the sample material was concentrated on 0.8-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane filter (ref:ATTP14250) on top of a Dacron pad mesh. A volume of 100 Litres was filtered in parallel on two 0.8-micrometre-pore-size filters. Each filter was then packaged in a sterile cryogenic tube (5 mL) (2 tubes, each with 1 filter), labelled with a barcode identification sticker and stored on board in liquid nitrogen. Note 1: Latex or nitril gloves were used for this protocol. Note 2: All containers, filter holders and tubing were washed with 0.1% bleach, rinsed twice with fresh water and rinsed again twice with the pre-filtered sample material. All tweezers are kept clean with ethanol. Note 3: The entire process did not exceed 15 minutes. Note 4: The total volume filtered on the two filters is 100 Litres. However, the volume filtered on each filter is unknown, but approximately 50 Litres. The intention of this protocol is to combine the two filters during molecular entities (nucleotides) extraction and sequencing analysis. A total of 2 (2x 2 tubes) replicates were prepared and sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

text: Rosette Vertical Sampling System [RVSS]

text: SV Tara

text: marine metagenome

text: TARA_20130524T1315Z_155_Combined-EVENTS_CAST_MES_(751m)_SEQ-(100L-or-15min)_W>0.8_N010000610

characteristic: title


characteristic: Chlorophyll Sensor

characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: GSC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

text: This sample (TARA_N010000604) was collected at station TARA_155 during campaign TARA_20130519Z of the Tara Oceans expedition (2009-2013), using a Rosette Vertical Sampling System [RVSS]. The sampling event (TARA_20130524T1315Z_155_Combined-EVENTS_CAST) occurred at position latitudeN=54.5742 and longitudeE=-16.8345, on date/time=2013-05-24T13:15:00, at a depth of 742-760 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [MES] marine water layer (ENVO:01000295) within the mesopelagic zone (ENVO:00000213). The sample material was size-fractionated (3-20 micrometres) and prepared on board using protocol [SEQ-(10-100L-or-15min)_W3-20] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity, metagenomics and/or transcriptomics studies of unicellular eukaryotes (protists) and attached prokaryotes. This sample has replicate sample(s): TARA_N010000605, TARA_N010000606, TARA_N010000603, TARA_N010000602.

text: Westerlies Biome

text: water

characteristic: longitude start

text: [NAO] North Atlantic Ocean (MRGID:1912)

characteristic: marine region

characteristic: nitrate sensor

text: marine metagenome

characteristic: oxygen sensor

text: Tara Oceans Expedition (2009-2013)

characteristic: project name

text: In the size fraction W3-20 micrometres, the sample material was pre-filtered on 20-micrometres, using a nylon mesh, and concentrated on 3-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane filter (ref:TSTP14250). A volume of 10-100 Litres was filtered and the 3-micrometre-pore-size filter was packaged in a sterile cryogenic tube (5 mL), labelled with a barcode identification sticker and stored on board in liquid nitrogen. Note 1: Latex or nitril gloves were used for this protocol. Note 2: All containers, filter holders and tubing were washed with 0.1% bleach, rinsed twice with fresh water and rinsed again twice with the pre-filtered sample material. All tweezers are kept clean with ethanol. Note 3: The entire process did not exceed 15 minutes. Note 4: The volume filtered is indicated for each replicate in the Sample registry (http://doi.pangaea.de/10.1594/PANGAEA.842197). A total of 2-6 replicates were prepared and sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

characteristic: protocol description

characteristic: protocol label

text: Rosette Vertical Sampling System [RVSS]

characteristic: sample collection device

characteristic: sample material label

characteristic: sample status

characteristic: sampling campaign

text: SV Tara

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: TARA_20130524T1315Z_155_Combined-EVENTS_CAST_MES_(751m)_SEQ-(10-100L-or-15min)_W3-20_N010000604


characteristic: Chlorophyll Sensor

characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: GSC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (TARA_N010000606) was collected at station TARA_155 during campaign TARA_20130519Z of the Tara Oceans expedition (2009-2013), using a Rosette Vertical Sampling System [RVSS]. The sampling event (TARA_20130524T1315Z_155_Combined-EVENTS_CAST) occurred at position latitudeN=54.5742 and longitudeE=-16.8345, on date/time=2013-05-24T13:15:00, at a depth of 742-760 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [MES] marine water layer (ENVO:01000295) within the mesopelagic zone (ENVO:00000213). The sample material was size-fractionated (3-20 micrometres) and prepared on board using protocol [SEQ-(10-100L-or-15min)_W3-20] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity, metagenomics and/or transcriptomics studies of unicellular eukaryotes (protists) and attached prokaryotes. This sample has replicate sample(s): TARA_N010000602, TARA_N010000603, TARA_N010000604, TARA_N010000605.

characteristic: description

text: Westerlies Biome

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (feature) further details

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

characteristic: event label

characteristic: further details

characteristic: last update date

characteristic: latitude start

text: [NAO] North Atlantic Ocean (MRGID:1912)

text: marine metagenome

characteristic: organism

text: Tara Oceans Expedition (2009-2013)

text: Rosette Vertical Sampling System [RVSS]

text: This version can be used to provide data discovery services

text: SV Tara

text: marine metagenome

text: TARA_20130524T1315Z_155_Combined-EVENTS_CAST_MES_(751m)_SEQ-(10-100L-or-15min)_W3-20_N010000606

characteristic: title


characteristic: Chlorophyll Sensor

characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: GSC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

text: This sample (TARA_R110002020) was collected at station TARA_155 during campaign TARA_20130519Z of the Tara Oceans expedition (2009-2013), using a Rosette Vertical Sampling System [RVSS]. The sampling event (TARA_20130524T1315Z_155_Combined-EVENTS_CAST) occurred at position latitudeN=54.5742 and longitudeE=-16.8345, on date/time=2013-05-24T13:15:00, at a depth of 742-760 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [MES] marine water layer (ENVO:01000295) within the mesopelagic zone (ENVO:00000213). The sample material was size-fractionated (<0.22 micrometres) and prepared on board using protocol [SEQ-(FeCl3)_W<0.22] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of viruses. This sample has no known replicate.

text: Westerlies Biome

text: water

characteristic: longitude start

text: [NAO] North Atlantic Ocean (MRGID:1912)

characteristic: marine region

characteristic: nitrate sensor

text: marine metagenome

characteristic: oxygen sensor

text: Tara Oceans Expedition (2009-2013)

characteristic: project name

text: In the size fraction W<0.22 micrometres, the sample material was pre-filtered using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane (ref:GPWP14250) with 0.22-micrometres-pore-size. A volume of 20 Litres was treated with FeCl3, incubated at room temperature for 1-24 hours to allow viruses to precipitate, filtered onto a 1-micrometre-pore-size, 142-mm-diameter GE polycarbonate (PC) membrane filter (ref:K10CP14220) placed on top of a 0.8-micrometre-pore-size, 142-mm-diameter Pall Gelman support filter (ref:60114) using a peristaltic pump with pressure <15 psi. The 1-micrometre-pore-size filter was then packaged in a sterile Falcon tube (50 mL) (up to 3 filters in 1 tube), labelled with a barcode identification sticker and stored on board in a refrigerator at +4 degree Celsius. Note 1: Latex or nitril gloves were used for this protocol. Note 2: All containers, filter holders and tubing were washed with 0.1% bleach, rinsed twice with fresh water and rinsed again twice with the pre-filtered sample material. All tweezers are kept clean with ethanol. Note 3: The three replicates were incubated together. For 20 Litres of filtrate, 2 millilitres of FeCl3 stock solution (4.83 grams of FeCl3-6H2O + 100 millilitres of MilliQ water, stored at 4°C) was added (final concentration of 1 mg Fe per Litre of filtrate), and shaken vigorously for 1 minute. Note 4: Further details about the method are published here doi:10.1111/j.1758-2229.2010.00208.x. In 2009-2012, a total of 3 replicates were prepared and sent for later analysis to the University of Arizona (UA), Tucson, USA. In 2013, a total of 2 replicates were prepared, one sent to for later analysis to the University of Arizona (UA), Tucson, USA, and the other sent for later analysis to the Observatoire Océanologique de Banyuls sur Mer (OOB), France.

characteristic: protocol description

characteristic: protocol label

text: Rosette Vertical Sampling System [RVSS]

characteristic: sample collection device

characteristic: sample material label

characteristic: sample status

characteristic: sampling campaign

text: SV Tara

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: TARA_20130524T1315Z_155_Combined-EVENTS_CAST_MES_(751m)_SEQ-(FeCl3)_W<0.22_R110002020


characteristic: Chlorophyll Sensor

characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: GSC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (TARA_B110000008) was collected at station TARA_155 during campaign TARA_20130519Z of the Tara Oceans expedition (2009-2013), using a high volume peristaltic pump [HVP-PUMP]. The sampling event (TARA_20130525T0907Z_155_EVENT_PUMP) occurred at position latitudeN=54.5451 and longitudeE=-16.7943, on date/time=2013-05-25T09:07:00, at a depth of 38-42 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [DCM] deep chlorophyll maximum layer (ENVO:01000326). The sample material was size-fractionated (0.22-3 micrometres) and prepared on board using protocol [SEQ-(100L)_W0.22-3] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes. This sample has replicate sample(s): TARA_B110000009.

characteristic: description

text: Westerlies Biome

characteristic: environment (biome)

characteristic: environment (feature)

text: discrete depth within the epipelagic zone (ENVO:00000209) within the photic zone (ENVO:00000209), near the base of a marine epipelagic wind mixed layer (ENVO:01000061)

characteristic: environment (feature) further details

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

characteristic: event label

characteristic: further details

characteristic: last update date

characteristic: latitude start

text: [NAO] North Atlantic Ocean (MRGID:1912)

text: marine metagenome

characteristic: organism

text: Tara Oceans Expedition (2009-2013)

text: high volume peristaltic pump [HVP-PUMP]

text: SV Tara

text: marine metagenome

text: TARA_20130525T0907Z_155_EVENT_PUMP_DCM_(40m)_SEQ-(100L)_W0.22-3_B110000008

characteristic: title


characteristic: Chlorophyll Sensor

characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: GSC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

text: This sample (TARA_N010000578) was collected at station TARA_155 during campaign TARA_20130519Z of the Tara Oceans expedition (2009-2013), using a high volume peristaltic pump [HVP-PUMP]. The sampling event (TARA_20130525T0907Z_155_EVENT_PUMP) occurred at position latitudeN=54.5451 and longitudeE=-16.7943, on date/time=2013-05-25T09:07:00, at a depth of 38-42 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [DCM] deep chlorophyll maximum layer (ENVO:01000326). The sample material was size-fractionated (>0.8 micrometres) and prepared on board using protocol [SEQ-(100L-or-15min)_W>0.8] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity, metagenomics and/or transcriptomics studies of unicellular eukaryotes (protists). This sample has replicate sample(s): TARA_N010000576, TARA_N010000577, TARA_N010000579.

text: Westerlies Biome

text: water (ENVO:00002006), including particulate matter (ENVO:01000060) such as planktonic material (ENVO:01000063)

text: water

characteristic: longitude start

text: [NAO] North Atlantic Ocean (MRGID:1912)

characteristic: marine region

characteristic: nitrate sensor

text: marine metagenome

characteristic: oxygen sensor

text: Tara Oceans Expedition (2009-2013)

characteristic: project name

characteristic: protocol description

characteristic: protocol label

text: high volume peristaltic pump [HVP-PUMP]

characteristic: sample collection device

characteristic: sample material label

characteristic: sample status

characteristic: sampling campaign

text: SV Tara

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: TARA_20130525T0907Z_155_EVENT_PUMP_DCM_(40m)_SEQ-(100L-or-15min)_W>0.8_N010000578


characteristic: Chlorophyll Sensor

text: Tara Oceans Consortium, Coordinators & Tara Oceans Expedition, Participants. Registry of all samples from the Tara Oceans Expedition (2009-2013). PANGAEA. doi:10.1594/PANGAEA.842197 (2015) (https://dx.doi.org/10.1594/PANGAEA.842197)

characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: GSC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

characteristic: Salinity Sensor

characteristic: Submitter Id

characteristic: depth

text: This sample (TARA_N010000591) was collected at station TARA_155 during campaign TARA_20130519Z of the Tara Oceans expedition (2009-2013), using a high volume peristaltic pump [HVP-PUMP]. The sampling event (TARA_20130525T0907Z_155_EVENT_PUMP) occurred at position latitudeN=54.5451 and longitudeE=-16.7943, on date/time=2013-05-25T09:07:00, at a depth of 38-42 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [DCM] deep chlorophyll maximum layer (ENVO:01000326). The sample material was size-fractionated (3-20 micrometres) and prepared on board using protocol [SEQ-(10-100L-or-15min)_W3-20] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity, metagenomics and/or transcriptomics studies of unicellular eukaryotes (protists) and attached prokaryotes. This sample has replicate sample(s): TARA_N010000592, TARA_N010000593, TARA_N010000590, TARA_N010000589.

characteristic: description

text: Westerlies Biome

characteristic: environment (biome)

characteristic: environment (feature)

characteristic: environment (feature) further details

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

characteristic: event label

characteristic: further details

characteristic: last update date

characteristic: latitude start

text: [NAO] North Atlantic Ocean (MRGID:1912)

text: marine metagenome

characteristic: organism

text: Tara Oceans Expedition (2009-2013)

text: high volume peristaltic pump [HVP-PUMP]

text: SV Tara

text: marine metagenome

text: TARA_20130525T0907Z_155_EVENT_PUMP_DCM_(40m)_SEQ-(10-100L-or-15min)_W3-20_N010000591

characteristic: title


characteristic: Chlorophyll Sensor

characteristic: Citation

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: Event Date/Time End

characteristic: External Id

text: GSC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

text: This sample (TARA_N010000593) was collected at station TARA_155 during campaign TARA_20130519Z of the Tara Oceans expedition (2009-2013), using a high volume peristaltic pump [HVP-PUMP]. The sampling event (TARA_20130525T0907Z_155_EVENT_PUMP) occurred at position latitudeN=54.5451 and longitudeE=-16.7943, on date/time=2013-05-25T09:07:00, at a depth of 38-42 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [DCM] deep chlorophyll maximum layer (ENVO:01000326). The sample material was size-fractionated (3-20 micrometres) and prepared on board using protocol [SEQ-(10-100L-or-15min)_W3-20] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity, metagenomics and/or transcriptomics studies of unicellular eukaryotes (protists) and attached prokaryotes. This sample has replicate sample(s): TARA_N010000589, TARA_N010000590, TARA_N010000591, TARA_N010000592.

text: Westerlies Biome

text: water

characteristic: longitude start

text: [NAO] North Atlantic Ocean (MRGID:1912)

characteristic: marine region

characteristic: nitrate sensor

text: marine metagenome

characteristic: oxygen sensor

text: Tara Oceans Expedition (2009-2013)

characteristic: project name

text: In the size fraction W3-20 micrometres, the sample material was pre-filtered on 20-micrometres, using a nylon mesh, and concentrated on 3-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane filter (ref:TSTP14250). A volume of 10-100 Litres was filtered and the 3-micrometre-pore-size filter was packaged in a sterile cryogenic tube (5 mL), labelled with a barcode identification sticker and stored on board in liquid nitrogen. Note 1: Latex or nitril gloves were used for this protocol. Note 2: All containers, filter holders and tubing were washed with 0.1% bleach, rinsed twice with fresh water and rinsed again twice with the pre-filtered sample material. All tweezers are kept clean with ethanol. Note 3: The entire process did not exceed 15 minutes. Note 4: The volume filtered is indicated for each replicate in the Sample registry (http://doi.pangaea.de/10.1594/PANGAEA.842197). A total of 2-6 replicates were prepared and sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.

characteristic: protocol description

characteristic: protocol label

text: high volume peristaltic pump [HVP-PUMP]

characteristic: sample collection device

characteristic: sample material label

characteristic: sample status

characteristic: sampling campaign

text: SV Tara

characteristic: sampling platform

characteristic: sampling station

text: marine metagenome

characteristic: size fraction lower threshold

characteristic: size fraction upper threshold

characteristic: temperature

text: TARA_20130525T0907Z_155_EVENT_PUMP_DCM_(40m)_SEQ-(10-100L-or-15min)_W3-20_N010000593