characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: The Westmead Institute for Medical Research and Marie Bashir Institute for Infectious Diseases and Biosecurity

text: public

text: Saudi Arabia

text: diseased

text: Homo sapiens

text: UPEC

text: urine

characteristic: isolation source

text: Escherichia coli

characteristic: organism

text: Escherichia coli

characteristic: strain

text: MDR UPEC RIYADH Chromosome sample 2

characteristic: title


status: PUBLIC

tag: attribute

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:The Westmead Institute for Medical Research and Marie Bashir Institute for Infectious Diseases and Biosecurity

characteristic: Submitter Id

characteristic: collection date

text: Saudi Arabia

characteristic: geographic location (country and/or sea)

text: diseased

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: UPEC

characteristic: isolate

text: urine

text: Escherichia coli

text: Escherichia coli

characteristic: serovar

text: MDR UPEC RIYADH Chromosome sample 3


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: Saudi Arabia

text: diseased

text: Homo sapiens

text: UPEC

text: urine

characteristic: isolation source

text: Escherichia coli

characteristic: organism

text: Escherichia coli

text: MDR UPEC RIYADH Chromosome sample 4


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: Saudi Arabia

text: diseased

text: Homo sapiens

text: UPEC

text: urine

text: Escherichia coli

text: Escherichia coli

characteristic: strain

text: MDR UPEC RIYADH Chromosome sample 5

characteristic: title


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Saudi Arabia

text: diseased

text: Homo sapiens

text: UPEC

text: urine

text: Escherichia coli

text: Escherichia coli

text: MDR UPEC RIYADH Chromosome sample 6


text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: Saudi Arabia

characteristic: geographic location (country and/or sea)

text: diseased

text: Homo sapiens

text: UPEC

text: urine

text: Escherichia coli

text: Escherichia coli

text: MDR UPEC RIYADH Chromosome sample 7


text: public

text: Saudi Arabia

text: diseased

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: UPEC

characteristic: isolate

text: urine

text: Escherichia coli

text: Escherichia coli

characteristic: serovar

text: MDR UPEC RIYADH Chromosome sample 8


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: Saudi Arabia

text: diseased

text: Homo sapiens

text: UPEC

text: urine

characteristic: isolation source

text: Escherichia coli

characteristic: organism

text: Escherichia coli

text: MDR UPEC RIYADH Chromosome sample 9


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: Saudi Arabia

text: diseased

text: Homo sapiens

text: UPEC

text: urine

text: Escherichia coli

text: Escherichia coli

characteristic: strain

text: MDR UPEC RIYADH Chromosome sample 10

characteristic: title


text: UNIVERSITY OF FREIBURG

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

tag: Namespace:UNIVERSITY OF FREIBURG

text: house mouse

characteristic: common name

text: DNA of Adult cardiomyocytes_rep1

characteristic: description

text: Mus musculus

text: Mus musculus


text: UNIVERSITY OF FREIBURG

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: house mouse

text: DNA of Adult cardiomyocytes_rep2

text: Mus musculus

text: Mus musculus


text: Griffith University

text: public

tag: Namespace:Griffith University

text: Joseph Adelskov

characteristic: collected by

text: Draft genome sequence of bacterial isolate produced from WGS sequencing

text: Australia

characteristic: geographic location (country and/or sea)

text: Spring Gully water treatment facility, Roma, Queensland

characteristic: geographic location (region and locality)

text: Joseph Adelskov

characteristic: identified by

characteristic: isolate

text: Coal seam gas extraction bore well

text: 25.999488 S 149.0704 E

characteristic: lat lon

text: Novosphingobium sp. NDB2Meth1

text: Novosphingobium sp. NDB2Meth1

text: Novosphingobium sp. NDB2Meth1 Draft Genome


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: IATA-CSIC

text: public

characteristic: collection date

text: The diversity and dynamics of yeasts associated with the fermentation of Argentinian maize-based beverage chicha was investigated. Samples taken at different stages from two chicha productions were analyzed by culture-dependent and culture-independent methods. Classical microbiological methods allowed isolation of five hundred ninety-six yeasts identified into 16 species by RFLPs and sequencing of D1/D2 26S rRNA gene. Genetic typing of isolates from the dominant species, Saccharomyces cerevisiae, by PCR of delta elements revealed up to 42 different patterns. High-throughput sequencing of D1/D2 26S rRNA gene amplicons from chicha samples allowed detection of more than one hundred yeast species and almost fifty filamentous fungi taxa. Analysis of the data revealed that yeasts dominated the fermentation, although, a significant percentage of filamentous fungi appeared in the first step of the process. Statistical examination of results showed that very few taxa were represented by more than 1% of the reads per sample at any step of the process. S. cerevisiae represented more than 90% of the reads in the fermentative samples. Other yeast species, different from S. cerevisiae, dominated the pre-fermentative steps and abounded in fermentative samples when S. cerevisiae was in percentages below 90%. Most yeasts species detected by pyrosequencing were not recovered by cultivation. In contrast, the cultivation-based methodology detected very few yeast taxa, and most of them corresponded with none or very few reads in the pyrosequencing analysis.

characteristic: environment (biome)

text: Andean plateau

characteristic: environment (feature)

text: maize beer

characteristic: environment (material)

text: Argentina

unit: m

characteristic: geographic location (elevation)

text: 23.37 S

unit: DD

characteristic: geographic location (latitude)

text: 65.24 W

characteristic: geographic location (longitude)

text: Maimara, Province Jujuy

text: mimarks-survey

characteristic: investigation type

text: miscellaneous natural or artificial environment

characteristic: miscellaneous environmental package

text: food fermentation metagenome

characteristic: organism

characteristic: project name

text: food fermentation metagenome

text: pyrosequencing

characteristic: sequencing method

text: 26S rRNA

characteristic: target gene

text: D1/D2 domains

characteristic: target subfragment

text: Yeast diversity during the fermentation of Andean chicha: a comparison between high-throughput sequencing and culture-dependent approaches.


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: IATA-CSIC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: description

text: Andean plateau

text: maize beer

text: Argentina

characteristic: geographic location (country and/or sea)

text: 23.27 S

text: 65.24 W

text: Maimara, Province Jujuy

characteristic: geographic location (region and locality)

text: mimarks-survey

text: food fermentation metagenome

text: food fermentation metagenome

text: pyrosequencing

text: 26S rRNA

text: D1/D2 domains

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: IATA-CSIC

text: public

characteristic: collection date

characteristic: environment (biome)

text: Andean plateau

characteristic: environment (feature)

text: maize beer

characteristic: environment (material)

text: Argentina

characteristic: geographic location (elevation)

text: 23.51 S

characteristic: geographic location (latitude)

text: 65.27 W

characteristic: geographic location (longitude)

text: Tumbaya, Province Jujuy

text: mimarks-survey

characteristic: investigation type

characteristic: miscellaneous environmental package

text: food fermentation metagenome

characteristic: organism

text: food fermentation metagenome

text: pyrosequencing

text: 26S rRNA

text: D1/D2 domains


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIMIB

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: metagenome

text: metagenome

text: Urban phyllosphere metagenomes, Magnolia

type: has member


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UNIMIB

text: public

text: metagenome

text: metagenome

text: Urban phyllosphere metagenomes, magnolia

characteristic: title


text: UNIMIB

text: public

text: metagenome

text: metagenome

text: Urban phyllosphere metagenomes, Cedrus


text: HELMHOLTZ CENTRE FOR INFECTION RESEARCH

text: public

tag: Namespace:HELMHOLTZ CENTRE FOR INFECTION RESEARCH

text: in vitro RNA-seq IP32953 LB exponential 25C replicate 1

characteristic: description

text: Yersinia pseudotuberculosis IP 32953

text: Yersinia pseudotuberculosis IP 32953


text: HELMHOLTZ CENTRE FOR INFECTION RESEARCH

text: public

text: in vitro RNA-seq IP32953 LB exponential 25C replicate 2

text: Yersinia pseudotuberculosis IP 32953

text: Yersinia pseudotuberculosis IP 32953


text: HELMHOLTZ CENTRE FOR INFECTION RESEARCH

text: public

text: in vitro RNA-seq IP32953 LB exponential 25C replicate 3

text: Yersinia pseudotuberculosis IP 32953

text: Yersinia pseudotuberculosis IP 32953


text: HELMHOLTZ CENTRE FOR INFECTION RESEARCH

text: public

text: in vitro RNA-seq IP32953 LB exponential 37C replicate 1

text: Yersinia pseudotuberculosis IP 32953

text: Yersinia pseudotuberculosis IP 32953


text: HELMHOLTZ CENTRE FOR INFECTION RESEARCH

text: public

text: in vitro RNA-seq IP32953 LB exponential 37C replicate 2

text: Yersinia pseudotuberculosis IP 32953

text: Yersinia pseudotuberculosis IP 32953


text: HELMHOLTZ CENTRE FOR INFECTION RESEARCH

text: public

text: in vitro RNA-seq IP32953 LB exponential 37C replicate 3

text: Yersinia pseudotuberculosis IP 32953

text: Yersinia pseudotuberculosis IP 32953


text: HELMHOLTZ CENTRE FOR INFECTION RESEARCH

text: public

text: in vitro RNA-seq IP32953 LB stationary 25C replicate 1

text: Yersinia pseudotuberculosis IP 32953

text: Yersinia pseudotuberculosis IP 32953


text: HELMHOLTZ CENTRE FOR INFECTION RESEARCH

text: public

text: in vitro RNA-seq IP32953 LB stationary 25C replicate 2

text: Yersinia pseudotuberculosis IP 32953

text: Yersinia pseudotuberculosis IP 32953


text: HELMHOLTZ CENTRE FOR INFECTION RESEARCH

text: public

text: in vitro RNA-seq IP32953 LB stationary 25C replicate 3

text: Yersinia pseudotuberculosis IP 32953

text: Yersinia pseudotuberculosis IP 32953


text: HELMHOLTZ CENTRE FOR INFECTION RESEARCH

text: public

text: in vitro RNA-seq IP32953 LB stationary 37C replicate 1

text: Yersinia pseudotuberculosis IP 32953

text: Yersinia pseudotuberculosis IP 32953


text: HELMHOLTZ CENTRE FOR INFECTION RESEARCH

text: public

text: in vitro RNA-seq IP32953 LB stationary 37C replicate 2

text: Yersinia pseudotuberculosis IP 32953

text: Yersinia pseudotuberculosis IP 32953


text: HELMHOLTZ CENTRE FOR INFECTION RESEARCH

text: public

text: in vitro RNA-seq IP32953 LB stationary 37C replicate 3

text: Yersinia pseudotuberculosis IP 32953

text: Yersinia pseudotuberculosis IP 32953


text: HELMHOLTZ CENTRE FOR INFECTION RESEARCH

text: public

characteristic: common name

text: RNA-seq IP32953 infected Peyers patches pooled runs biological replicate 1 (includes ERCC ExFold RNA SpikeIn Mix 1)

text: Yersinia pseudotuberculosis IP 32953

text: Yersinia pseudotuberculosis IP 32953


text: HELMHOLTZ CENTRE FOR INFECTION RESEARCH

text: public

text: RNA-seq IP32953 infected Peyers patches pooled runs biological replicate 2 (includes ERCC ExFold RNA SpikeIn Mix 2)

text: Yersinia pseudotuberculosis IP 32953

text: Yersinia pseudotuberculosis IP 32953


text: HELMHOLTZ CENTRE FOR INFECTION RESEARCH

text: public

text: RNA-seq IP32953 infected Peyers patches pooled runs biological 3 (includes ERCC ExFold RNA SpikeIn Mix 1)

text: Yersinia pseudotuberculosis IP 32953

text: Yersinia pseudotuberculosis IP 32953


text: HELMHOLTZ CENTRE FOR INFECTION RESEARCH

text: public

text: house mouse

text: RNA-seq IP32953 infected Peyers patches pooled runs biological (includes ERCC ExFold RNA SpikeIn Mix 1)

text: Mus musculus

text: Mus musculus


text: HELMHOLTZ CENTRE FOR INFECTION RESEARCH

text: public

text: house mouse

text: Mus musculus

text: Mus musculus


text: HELMHOLTZ CENTRE FOR INFECTION RESEARCH

text: public

text: house mouse

text: RNA-seq IP32953 infected Peyers patches pooled runs biological replicate 3 (includes ERCC ExFold RNA SpikeIn Mix 1)

text: Mus musculus

text: Mus musculus


text: HELMHOLTZ CENTRE FOR INFECTION RESEARCH

text: public

text: RNA-seq uninfected Peyers patches pooled runs biological replicate 1 (includes ERCC ExFold RNA SpikeIn Mix 2)

text: Yersinia pseudotuberculosis IP 32953

text: Yersinia pseudotuberculosis IP 32953


text: HELMHOLTZ CENTRE FOR INFECTION RESEARCH

text: public

text: RNA-seq uninfected Peyers patches pooled runs biological replicate 2 (includes ERCC ExFold RNA SpikeIn Mix 1)

text: Yersinia pseudotuberculosis IP 32953

text: Yersinia pseudotuberculosis IP 32953


text: HELMHOLTZ CENTRE FOR INFECTION RESEARCH

text: public

text: RNA-seq uninfected Peyers patches pooled runs biological replicate 3 (includes ERCC ExFold RNA SpikeIn Mix 2)

text: Yersinia pseudotuberculosis IP 32953

text: Yersinia pseudotuberculosis IP 32953


text: HELMHOLTZ CENTRE FOR INFECTION RESEARCH

text: public

text: house mouse

text: RNA-seq uninfected Peyers patches pooled runs biological (includes ERCC ExFold RNA SpikeIn Mix 2)

text: Mus musculus

text: Mus musculus


text: HELMHOLTZ CENTRE FOR INFECTION RESEARCH

text: public

text: house mouse

text: RNA-seq uninfected Peyers patches pooled runs biological (includes ERCC ExFold RNA SpikeIn Mix 1)

text: Mus musculus

text: Mus musculus


text: public

tag: Namespace:ALL-RUSSIA RESEARCH INSTITUTE FOR AGRICULTURAL MICROBIOLOGY

text: pea

characteristic: genotype

text: Pisum sativum

text: nodules

characteristic: organism part

text: 3 weeks post inoculation

characteristic: sampling time point

text: Pisum sativum


text: HOSPICES CIVILS DE LYON

text: public

tag: Namespace:HOSPICES CIVILS DE LYON

characteristic: collection date

text: France

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: host health state

text: homo sapiens

characteristic: host scientific name

characteristic: isolate

text: blood

characteristic: isolation source

text: Staphylococcus aureus

characteristic: organism

text: Staphylococcus aureus

characteristic: strain

text: MSSA tirS positive


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: qiita_sid_10483:10483.recip.452.ASO.PD3.D7

characteristic: Submitter Id

characteristic: age

text: years

characteristic: age units

text: Missing: Not provided

characteristic: anonymized name

characteristic: bmi

text: UBERON:feces

characteristic: body habitat

text: UBERON:feces

characteristic: body product

text: UBERON:feces

characteristic: body site

text: Missing: Not provided

characteristic: cage id

text: Missing: Not provided

text: Missing: Not provided

characteristic: cohoused

text: Missing: Restricted access

text: Missing: Not provided

text: Missing: Not provided

text: Role of Gut Microbiota in Pathophysiology of Parkinson's Disease

characteristic: disease duration

text: PD

characteristic: disease status

text: TRUE

text: PD

characteristic: donor status

characteristic: elevation

text: urban biome

characteristic: env biome

text: human-associated habitat

characteristic: env feature

text: human-gut

characteristic: env package

text: feces

text: Missing: Not provided

text: Missing: Not provided

text: USA:CA:Pasadena

characteristic: geo loc name

text: Not provided

characteristic: height

text: Missing: Not provided

text: Homo sapiens

characteristic: host subject id

characteristic: host taxid

characteristic: latitude

text: adult

characteristic: life stage

characteristic: longitude

text: Missing: Not provided

text: Missing: Not provided

text: Missing: Not provided

text: Sertaline 150mg / day: Buproprion XL 300 mg / day

characteristic: medications

text: Missing: Not provided

text: human gut metagenome

text: Missing: Not provided

text: Missing: Not provided

text: UCSDMI

text: TRUE

text: Caucasian

characteristic: race

text: stool

characteristic: sample type

text: human gut metagenome

text: Missing: Not provided

text: Missing: Not provided

text: Missing: Not provided

text: male

characteristic: sex

text: Missing: Not provided


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NEW ENGLAND BIOLABS

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:NEW ENGLAND BIOLABS

characteristic: Submitter Id

text: human

characteristic: common name

text: DNA is diluted in water (pH 5.81) and fragmented to 200bp by acoustic shearing, followed by DNA library prep without DNA repair using NEBNext DNA Library Prep Master Mix for Illumina.

characteristic: description

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: male

text: DNA sheared in water for validation

characteristic: title


text: NEW ENGLAND BIOLABS

text: public

text: human

text: DNA is diluted in water (pH 5.81), fragmented to 200bp by acoustic shearing, and treated with NEBNext FFPE DNA Repair mix, followed by DNA library preparation using NEBNext DNA Library Prep Master Mix for Illumina

text: Homo sapiens

text: Homo sapiens

text: male

text: DNA sheared in water PreCR treated


Validation_0.1X TE_shear_no_repair

text: NEW ENGLAND BIOLABS

text: public

text: Validation_0.1X TE_shear_no_repair

text: human

text: DNA is diluted in 0.1X TE (pH8) and fragmented to 200bp by acoustic shearing, followed by DNA library prep without DNA repair using NEBNext DNA Library Prep Master Mix for Illumina.

text: Homo sapiens

text: Homo sapiens

text: male

text: DNA sheared in 0.1X TE


Validation_0.1X TE_shear_repair

text: NEW ENGLAND BIOLABS

text: public

text: Validation_0.1X TE_shear_repair

text: human

text: DNA is diluted in 0.1X TE (pH8), fragmented to 200bp by acoustic shearing, and treated with NEBNext FFPE DNA Repair mix, followed by DNA library preparation using NEBNext DNA Library Prep Master Mix for Illumina

text: Homo sapiens

text: Homo sapiens

text: male

text: DNA sheared in 0.1X TE Repair


Validation_1X TE_shear_no_repair

text: NEW ENGLAND BIOLABS

text: public

text: Validation_1X TE_shear_no_repair

text: human

text: DNA is diluted in 1X TE (pH8) and fragmented to 200bp by acoustic shearing, followed by DNA library prep without DNA repair using NEBNext DNA Library Prep Master Mix for Illumina.

text: Homo sapiens

text: Homo sapiens

text: male

text: DNA sheared in 1X TE


Validation_1X TE_shear_repair

text: NEW ENGLAND BIOLABS

text: public

text: Validation_1X TE_shear_repair

text: human

text: DNA is diluted in 1X TE (pH8), fragmented to 200bp by acoustic shearing, and treated with NEBNext FFPE DNA Repair mix, followed by DNA library preparation using NEBNext DNA Library Prep Master Mix for Illumina.

text: Homo sapiens

text: Homo sapiens

text: male

text: DNA sheared in 1X TE repaired


text: NEW ENGLAND BIOLABS

text: public

text: Buffer_composition_water_shear_no_repair

text: human

text: DNA is diluted in water (pH 5.81) and fragmented to 200bp by acoustic shearing, followed by DNA library prep without DNA repair using NEBNext Ultra II DNA Library Prep kit for Illumina.

text: Homo sapiens

text: Homo sapiens

text: male

text: DNA sheared in water


text: NEW ENGLAND BIOLABS

text: public

text: human

text: DNA is diluted in water (pH 5.81), fragmented to 200bp by acoustic shearing, and treated with NEBNext FFPE DNA Repair mix, followed by DNA library preparation using NEBNext Ultra II DNA Library Prep kit for Illumina.

text: Homo sapiens

text: Homo sapiens

text: male

text: DNA sheared in water repaired


text: NEW ENGLAND BIOLABS

text: public

text: Buffer_composition_1 mM Tris_shear_no_repair

text: human

text: DNA is diluted in 1 mM Tris (pH8), and fragmented to 200bp by acoustic shearing, followed by DNA library preparation using NEBNext Ultra II DNA Library Prep kit for Illumina.

text: Homo sapiens

text: Homo sapiens

text: male

text: DNA sheared in 1 mM Tris


name: Buffer_composition_1 mM Tris, 0.1 mM EDTA_shear_no_repair

text: NEW ENGLAND BIOLABS

text: public

text: human

text: DNA is diluted in 1 mM Tris, 0.1 mM EDTA (pH8), and fragmented to 200bp by acoustic shearing, followed by DNA library preparation using NEBNext Ultra II DNA Library Prep kit for Illumina.

text: Homo sapiens

text: Homo sapiens

text: male

text: DNA sheared in 1 mM Tris, 0.1 mM EDTA


text: NEW ENGLAND BIOLABS

text: public

text: Buffer_composition_10 mM Tris_shear_no_repair

text: human

text: DNA is diluted in 10 mM Tris (pH8), and fragmented to 200bp by acoustic shearing, followed by DNA library preparation using NEBNext Ultra II DNA Library Prep kit for Illumina.

text: Homo sapiens

text: Homo sapiens

text: male

text: DNA sheared in 10 mM Tris


name: Buffer_composition_10 mM Tris, 0.1 mM EDTA_shear_no_repair

text: NEW ENGLAND BIOLABS

text: public

text: human

text: DNA is diluted in 10 mM Tris, 0.1 mM EDTA (pH8), and fragmented to 200bp by acoustic shearing, followed by DNA library preparation using NEBNext Ultra II DNA Library Prep kit for Illumina.

text: Homo sapiens

text: Homo sapiens

text: male

text: DNA sheared in 10 mM Tris, 0.1 mM EDTA


name: Buffer_composition_10 mM Tris, 1mM EDTA_shear_no_repair

text: NEW ENGLAND BIOLABS

text: public

text: human

text: DNA is diluted in 10 mM Tris, 1 mM EDTA (pH8), and fragmented to 200bp by acoustic shearing, followed by DNA library preparation using NEBNext Ultra II DNA Library Prep kit for Illumina

text: Homo sapiens

text: Homo sapiens

text: male

text: DNA sheared in 10 mM Tris, 1 mM EDTA


text: NEW ENGLAND BIOLABS

text: public

text: Target_enrichment_0.1X TE_shear_no_repair

text: human

text: DNA is diluted in 0.1X TE (pH8), and fragmented to 200bp by acoustic shearing, followed by DNA library preparation using NEBNext Ultra II DNA Library Prep kit for Illumina and SureSelect Target Enrichment kit (Agilent).

text: Homo sapiens

text: Homo sapiens

text: male

text: DNA sheared in 0.1X TE enriched


Target_enrichment_0.1X TE_shear_repair

text: NEW ENGLAND BIOLABS

text: public

text: Target_enrichment_0.1X TE_shear_repair

text: human

text: DNA is diluted in 0.1X TE (pH8), fragmented to 200bp by acoustic shearing, and treated with NEBNext FFPE DNA Repair mix, followed by DNA library preparation using NEBNext Ultra II DNA Library Prep kit for Illumina and SureSelect Target Enrichment kit (Agilent).

text: Homo sapiens

text: Homo sapiens

text: male

text: DNA sheared in 0.1X TE repaired enriched


text: University of Jyvaskyla

text: public

tag: Namespace:University of Jyvaskyla

characteristic: collection date

text: soil

text: arcto-alpine tundra biome

characteristic: environment (biome)

text: alpine tundra

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: Austria

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: metagenome

text: plant-associated

characteristic: plant-associated environmental package

text: Plants Assemble Species Specific Bacterial Communities From Common Core Taxa in Three Arcto-Alpine Climate Zones

characteristic: project name

text: metagenome

text: Ion-Torrent

characteristic: sequencing method


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Jyvaskyla

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: rhizosphere soil

text: arcto-alpine tundra biome

text: alpine tundra

text: soil

text: Austria

text: metagenome

text: metagenome

text: plant-associated

text: metagenome

text: Ion-Torrent


text: University of Jyvaskyla

text: public

characteristic: Submitter Id

characteristic: collection date

text: endophyte

characteristic: description

text: arcto-alpine tundra biome

characteristic: environment (biome)

text: alpine tundra

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: Austria

text: metagenome

text: metagenome

characteristic: organism

text: plant-associated

text: metagenome

text: Ion-Torrent

characteristic: title


characteristic: ENA-CHECKLIST

text: University of Jyvaskyla

text: public

text: endophyte

text: arcto-alpine tundra biome

text: arctic tundra

text: soil

text: Finland

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: metagenome

text: plant-associated

characteristic: plant-associated environmental package

characteristic: project name

text: metagenome

text: Ion-Torrent

characteristic: sequencing method


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Jyvaskyla

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: soil

text: arcto-alpine tundra biome

text: arctic tundra

text: soil

text: Finland

text: metagenome

text: metagenome

text: plant-associated

text: metagenome

text: Ion-Torrent


text: University of Jyvaskyla

text: public

characteristic: Submitter Id

characteristic: collection date

text: rhizosphere soil

characteristic: description

text: arcto-alpine tundra biome

characteristic: environment (biome)

text: arctic tundra

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: Finland

text: metagenome

text: metagenome

characteristic: organism

text: plant-associated

text: metagenome

text: Ion-Torrent

characteristic: title


characteristic: ENA-CHECKLIST

text: University of Jyvaskyla

text: public

text: soil

text: arcto-alpine tundra biome

text: arctic tundra

text: soil

text: Norway

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: metagenome

text: plant-associated

characteristic: plant-associated environmental package

characteristic: project name

text: metagenome

text: Ion-Torrent

characteristic: sequencing method


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Jyvaskyla

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: rhizosphere soil

text: arcto-alpine tundra biome

text: arctic tundra

text: soil

text: Norway

text: metagenome

text: metagenome

text: plant-associated

text: metagenome

text: Ion-Torrent


text: University of Jyvaskyla

text: public

characteristic: Submitter Id

characteristic: collection date

text: endophyte

characteristic: description

text: arcto-alpine tundra biome

characteristic: environment (biome)

text: arctic tundra

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: Norway

text: metagenome

text: metagenome

characteristic: organism

text: plant-associated

text: metagenome

text: Ion-Torrent

characteristic: title


characteristic: ENA-CHECKLIST

text: public

tag: Namespace:Oxford University Clinical Research Unit

characteristic: collected by

text: Whole genome sequencing of C. neoformans var. grubii from HIV-infected and HIV-uninfected patients in Vietnam

text: No

characteristic: environmental sample

text: Viet Nam

characteristic: geographic location (country and/or sea)

text: 10.8231° N

characteristic: geographic location (latitude)

text: 106.6297° E

characteristic: geographic location (longitude)

text: diseased

characteristic: host health state

text: Homo Sapiens

characteristic: host scientific name

text: Yes

characteristic: is the sequenced pathogen host associated?

text: Clinical

characteristic: isolate

text: CSF

characteristic: isolation source host associated

text: alpha

characteristic: mating type

text: Cryptococcus neoformans var. grubii

text: Cryptococcus neoformans var. grubii

characteristic: strain

text: Cryptococcus neoformans


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Oxford University Clinical Research Unit

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

characteristic: description

text: No

text: Viet Nam

text: 10.8231° N

text: 106.6297° E

text: diseased

text: Homo Sapiens

text: Yes

text: Clinical

text: CSF

text: alpha

text: Cryptococcus neoformans var. grubii

text: Cryptococcus neoformans var. grubii

text: Cryptococcus neoformans


characteristic: ENA-CHECKLIST

text: ARO

text: public

text: root

characteristic: environment (biome)

text: root

characteristic: environment (feature)

text: root

characteristic: environment (material)

text: Israel

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: metagenome

characteristic: organism

text: plant-associated

characteristic: plant-associated environmental package

text: Root associated bacteria_sub-sample1

characteristic: project name

text: metagenome

text: Illumina

characteristic: sequencing method

text: sub-sample 1

characteristic: title


text: Propionibacterium freudenreichii subsp. shermanii

characteristic: ArrayExpress-Species

characteristic: ArrayExpress-StrainOrLine

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: common name

characteristic: strain


characteristic: ENA-CHECKLIST

text: URMITE

text: public

characteristic: Submitter Id

text: Obese Amazonie

characteristic: description

text: Bacteria acuminatocercata

characteristic: organism

text: Bacteria acuminatocercata


text: public

tag: Namespace:Department of Plant and Microbial Biology

text: ArrayExpress

characteristic: broker name

text: thale cress

text: Protocols: Ler and Cvi-0 were reciprocally crossed to generate Ler/Cvi-0 hybrids. These were then used to pollinate homozygous mea/mea or Ler plants. Three independent replicates were generated, each using different parental individuals and at different days. In total, around 10,000 and 4,000 F2 seeds were generated frommea/mea and Ler mothers, respectively. Seeds were surface sterilised for 10 minutes using 1% sodium hypochlorite, washed extensively with water, and sown in MS medium. After 10-14 days of growth at 22°C, leaves from viable seedlings were collected (825, 800, and 775 seedlings for each of theWTpool replicates; 600, 400, and 400 individuals for each of the mea pool replicates). DNA was extracted in groups of 50 leaves using the DNeasy Plant Mini Kit (Qiagen cat. 69104), and quantified using the Qubit dsDNA HS Assay kit (Life Technologies cat. Q32854). DNA from the different extractions was pooled in equi-amounts, precipitated using sodium acetate and isopropanol, and resuspended in 25 μl TE buffer for each replicate at a final concentration of 100-150 ng/μl. The TruSeq DNA Sample Prep Kit v2 (Illumina) was used in the succeeding steps. DNA sam- ples (1 μg) were sonicated and the fragmented DNA samples end-repaired and polyadenylated. TruSeq adapters containing the index for multiplexing were ligated to the fragmented DNA samples. The ligated samples were run on a 2%agarose gel and the desired fragment length was excised (50bp +/- the target fragment length). DNA from the gel was purified with MinE- lute Gel Extraction Kit (Qiagen). Fragments containing TruSeq adapters on both ends were selectively enriched with PCR. The quality and quantity of the enriched libraries were validated using Qubit (1.0) Fluorometer and the Caliper GX LabChip GX (Caliper Life Sciences). The product is a smear with an average fragment size of approximately 260 bp. The libraries were normalized to 10nM in Tris-Cl 10 mM, pH8.5 with 0.1% Tween 20.

text: mea/mea x [Ler/Cvi hybrid]

characteristic: genotype

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: Plant Leaves

characteristic: organism part

text: Arabidopsis thaliana

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Department of Plant and Microbial Biology

text: public

text: ArrayExpress

text: thale cress

text: mea/mea x [Ler/Cvi hybrid]

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: Plant Leaves

text: Arabidopsis thaliana


characteristic: External Id

characteristic: INSDC center name

text: public

text: ArrayExpress

text: thale cress

text: Ler x [Ler/Cvi hybrid]

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: Plant Leaves

text: Arabidopsis thaliana


text: URMITE

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: Obese Polynesie

text: Bacteria aborigena

text: Bacteria aborigena


text: URMITE

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Beer microbiota of Obese people

text: Bacteria aborigena

text: Bacteria aborigena


text: AALBORG UNIVERSITY

text: public

tag: Namespace:AALBORG UNIVERSITY

text: metagenome

text: metagenome


soil

text: AALBORG UNIVERSITY

text: public

text: soil

text: metagenome

text: metagenome

text: soil


text: Luxembourg Institute of Health

text: public

tag: Namespace:Luxembourg Institute of Health

text: house mouse

characteristic: common name

text: MspI digestion of splenocyte gDNA from Mus musculus exposed to H1N1 i.n. at PND14

text: Mus musculus

text: Mus musculus

text: PND14 H1N1 exposure, MspI digested


characteristic: ENA-CHECKLIST

text: Luxembourg Institute of Health

text: public

text: house mouse

text: HpaII digestion of splenocyte gDNA from Mus musculus exposed to H1N1 i.n. at PND14

text: Mus musculus

text: Mus musculus

text: PND14 H1N1 exposure, HpaII digested


text: Luxembourg Institute of Health

text: public

characteristic: Submitter Id

text: house mouse

text: MspI digestion of splenocyte gDNA from Mus musculus exposed to saline i.n. (control) at PND14

text: Mus musculus

text: Mus musculus

text: PND14 Saline exposure, MspI digested


text: Luxembourg Institute of Health

text: public

text: house mouse

text: HpaII digestion of splenocyte gDNA from Mus musculus exposed to saline i.n. (control) at PND14

characteristic: description

text: Mus musculus

text: Mus musculus

text: PND14 H1N1 exposure, MspI digested


text: Univeristy of Aberdeen

text: public

tag: Namespace:Univeristy of Aberdeen

characteristic: collection date

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: diseased

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

characteristic: isolate

text: Biopsy

characteristic: isolation source

text: Campylobacter showae

characteristic: organism

text: Campylobacter showae

text: Campylobacter showae strain from a biopsy of a Paediatric patient with Crohn's disease


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Univeristy of Aberdeen

text: public

text: United Kingdom

text: diseased

text: Homo sapiens

text: Biopsy

text: Campylobacter showae

text: Campylobacter showae

text: Campylobacter showae strain from a biopsy of an Adult patient with a colonic Adenoma

characteristic: title


text: Univeristy of Aberdeen

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: United Kingdom

text: diseased

text: Homo sapiens

text: CAM

text: Stool

text: Campylobacter showae

text: Campylobacter showae

text: Campylobacter showae strain from a stool sample of an Adult patient with gastroenteritis


characteristic: ENA-CHECKLIST

text: UNIVERSITY OF SZEGED

text: public

characteristic: SRA accession

tag: Namespace:UNIVERSITY OF SZEGED

characteristic: Submitter Id

text: Suid herpesvirus 1 strain Kaplan

text: Suid herpesvirus 1 strain Kaplan

text: Dynamic characterization of the PRV transcriptome


text: public

tag: Namespace:Environmental and Applied Microband Applied Microb

characteristic: collection date

text: Bacteria

characteristic: description

text: terrene

characteristic: environment (biome)

text: grassland

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: China

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: metagenome

text: microbial community

characteristic: project name

text: metagenome

text: MiSeq

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: temperate grassland


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Environmental and Applied Microband Applied Microb

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Bacteria

text: terrene

text: grassland

text: soil

text: China

text: metagenome

text: metagenome

characteristic: organism

text: microbial community

text: metagenome

text: MiSeq

text: soil

text: temperate grassland

characteristic: title


text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: Fungi

characteristic: description

text: terrene

characteristic: environment (biome)

text: grassland

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: China

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

text: metagenome

text: metagenome

text: microbial community

text: metagenome

text: MiSeq

text: soil

text: temperate grassland


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

tag: Namespace:Institute for molecular and tumor biology Philipps University  Marburg Germany

text: ArrayExpress

characteristic: broker name

text: tumor associated macrophage

characteristic: cell type

text: human

characteristic: common name

text: Protocols: Ascites was collected from patients with high-grade serous ovarian carcinoma undergoing primary surgery at the University Hospital in Marburg. Informed consent was obtained from all patients according to the protocols approved by the local ethical committe. Cells were extract by CD14 MACS. After Ficoll gradient centrifugation, TAMs were purified by CD14 MACS and lysed in Peqgold (TRIzol-like solution based on phenol and chloroform). RNA was digested with DNase (MN) and purified on a Qiagen Minelute column. Illumina-compatible libraries were made using the Epicentre Scriptseq human/mouse/rat low input Gold kit. Libraries were created using the Illumina Truseq mRNA kit v2 used according to the manufacturer's instructions.

text: ovarian carcinoma

characteristic: disease

characteristic: individual

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens


tag: attribute

text: Institute for molecular and tumor biology Philipps University Marburg Germany

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: tumor associated macrophage

text: human

text: ovarian carcinoma

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Homo sapiens

characteristic: title


characteristic: ENA-CHECKLIST

text: GREGOR MENDEL INSTITUTE

text: public

tag: Namespace:GREGOR MENDEL INSTITUTE

text: Brachypodium hybridum

text: Brachypodium hybridum


text: Plasmodium falciparum

characteristic: ArrayExpress-Species

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: malaria parasite P. falciparum

text: Plasmodium falciparum

text: Plasmodium falciparum


text: Streptococcus suis

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

text: public

text: Streptococcus suis

text: Streptococcus suis

text: Streptococcus suis


text: Institute for Molecular Biosciences

text: public

tag: Namespace:Institute for Molecular Biosciences

text: BRAEMBL

characteristic: broker name

characteristic: collection date

text: Nucleic acid sequences and peptide sequences from venom-gland preparations of the spider Liphistius malayanus

characteristic: description

text: Switzerland

characteristic: geographic location (country and/or sea)

text: Geneva

characteristic: geographic location (region and locality)

characteristic: lat lon

characteristic: library reads sequenced

text: Liphistius malayanus

text: Aerobe

characteristic: relationship to oxygen

text: Spider specimens bought from a supplier in Geneva, Switzerland.

characteristic: sample material processing

text: Liphistius malayanus

text: Liphistius malayanus


text: Uni Research

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:Uni Research

characteristic: Submitter Id

text: baker's yeast

characteristic: common name

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: BY4741 adapted to NaAsO2 6mM - Replicate 1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Uni Research

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

characteristic: organism

text: Saccharomyces cerevisiae

text: BY4741 adapted to NaAsO2 6mM - Replicate 2

characteristic: title


text: Uni Research

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: BY4741 adapted to NaAsO2 6mM - Replicate 3


text: Uni Research

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: BY4741 adapted to NaAsO2 6mM - Replicate 4


text: Uni Research

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: BY4741 adapted to CdCl2 0.2 mM - Replicate 1


text: Uni Research

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: BY4741 adapted to CdCl2 0.2 mM - Replicate 2


text: Uni Research

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: BY4741 adapted to CdCl2 0.2 mM - Replicate 3


text: Uni Research

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: BY4741 adapted to CdCl2 0.2 mM - Replicate 4


text: Uni Research

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: BY4741 adapted to CuCl2 1 mM - Replicate 1


text: Uni Research

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: BY4741 adapted to CuCl2 1 mM - Replicate 2


text: Uni Research

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: BY4741 adapted to CuCl2 1 mM - Replicate 3


text: Uni Research

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: BY4741 adapted to CuCl2 1 mM - Replicate 4


text: Uni Research

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: BY4741 adapted to Ethanol 8% - Replicate 1


text: Uni Research

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: BY4741 adapted to Ethanol 8% - Replicate 2


text: Uni Research

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: BY4741 adapted to Ethanol 8% - Replicate 3


text: Uni Research

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: BY4741 adapted to Ethanol 8% - Replicate 4


text: Uni Research

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: BY4741 adapted to MgCl2 664 mM - Replicate 1


text: Uni Research

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: BY4741 adapted to MgCl2 664 mM - Replicate 2


text: Uni Research

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: BY4741 adapted to MgCl2 664 mM - Replicate 3


text: Uni Research

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: BY4741 adapted to MgCl2 664 mM - Replicate 4


text: Uni Research

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: BY4741 adapted to NaCl 1.4 M - Replicate 1


text: Uni Research

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: BY4741 adapted to NaCl 1.4 M - Replicate 2


text: Uni Research

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: BY4741 adapted to NaCl 1.4 M - Replicate 3


text: Uni Research

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: BY4741 adapted to NaCl 1.4 M - Replicate 4


text: Uni Research

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: BY4741 adapted to Na2SeO3 1 mM - Replicate 1


text: Uni Research

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: BY4741 adapted to Na2SeO3 1 mM - Replicate 2


text: Uni Research

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: BY4741 adapted to Na2SeO3 1 mM - Replicate 3


text: Uni Research

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: BY4741 adapted to Na2SeO3 1 mM - Replicate 4


text: Uni Research

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: BY4741 adapted to LiCl 300 mM - Replicate 1


text: Uni Research

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: BY4741 adapted to LiCl 300 mM - Replicate 2


text: Uni Research

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: BY4741 adapted to LiCl 300 mM - Replicate 3


text: Uni Research

text: public

text: baker's yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: BY4741, wild type


text: public

tag: Namespace:Unidad de Metabolismo Mineral, Instituto Maimonides de Investigacion Biomedica de Cordoba (IMIBIC), Hospital Universitario Reina Sofia, Avda. Menendez Pidal s/n, 14004 Cordoba,

text: ArrayExpress

characteristic: broker name

text: adipocyte

characteristic: cell type

text: human

text: Protocols: Bone-marrow human mesenchymal stromal-cells were obtained from three donors recruited in the Bone-Marrow Transplant Program of Hematology Service at Reina Sofía University Hospital (Córdoba, Spain), after signed written informed-consent. MSC were cultured in Minimum Essential-Medium Alpha (MEM α) Eagle from Lonza (Basel, Switzerland), supplemented with 10% fetal bovine-serum (FBS) from Gibco - Life Technologies - Thermo Fisher Scientific (Waltham, MA, USA), 2 mM UltraGlutamine (Lonza), 1 ng basic fibroblast-growth factor (bFGF)/ml from Sigma-Aldrich (Saint Louis, MO, USA), 100 U ampicillin and 0.1 mg streptomycin/ml. Cells were grown at 37 ºC and 5% CO2 in humidity. Cultures grown until about 80 to 90% confluence were maintained in MEMα plus 10% FBS without bFGF (controls, C) or likewise but supplemented with 5x10-7 M dexamethasone, 0.5 mM isobutylmethylxanthine and 50 mM indomethacin (Sigma-Aldrich) adipogenic inductors for cell differentiation, in absence (A) or presence (A+O) 10-5 M oleuropein (purity >80%) from Extrasynthese (Lyon, France). Adipogenic differentiation at day 14 was determined by oil-red O staining of lipid droplets. In short, cells were fixed with 3.7% formaldehyde, washed with 60% isopropanol in water and stained with a mixture of 60% of 0.3% (w/v in isopropanol) of oil-red O and 40% distilled water for 20 minutes. They were then washed with water, stained with hematoxylin (all chemicals from Sigma-Aldrich) and visualized under a light microscope. Total RNA was isolated at days seven and 14 after adipogenesis induction, using mirVana miRNA Isolation Kit from Ambion - Life Technologies - Thermo Fisher Scientific. RNA were quantified using a ND-1000 spectrophotometer from NanoDrop - Thermo Fisher Scientific. Integrity was checked by agarose-gel electrophoresis (AGE). RNA samples were pooled from each time and donor, to generate three samples: control (C), adipocytes (A) and adipocytes plus oleuropein (A+O) for gene-expression analyses.

characteristic: description

text: Homo sapiens

text: Homo sapiens

text: bone marrow

characteristic: organism part

text: mesenchymal stem cell

characteristic: progenitor cell type

text: Homo sapiens


text: Unidad de Metabolismo Mineral, Instituto Maimonides de Investigacion Biomedica de Cordoba (IMIBIC), Hospital Universitario Reina Sofia, Avda. Menendez Pidal s/n, 14004 Cordoba,

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ArrayExpress

text: adipocyte

text: human

text: Homo sapiens

text: Homo sapiens

text: bone marrow

text: mesenchymal stem cell

text: Homo sapiens


characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ArrayExpress

text: mesenchymal stem cell

text: human

text: Homo sapiens

text: Homo sapiens

text: bone marrow

text: mesenchymal stem cell

text: Homo sapiens


E-MTAB-5221:MEK28 steady state

text: public

characteristic: SRA accession

text: E-MTAB-5221:MEK28 steady state

tag: Namespace:Department of Biology University of Copenhagen

characteristic: Submitter Id

text: ArrayExpress

text: Protocols: Cells harvested right before and 10 min after valine addition. 280 ml of cultures were harvested, centrifuged and washed in 0° C biotin-free medium 10 generations of growth on MOPS minimal medium supplemented with 0.2% glucose and 5 µg/ml biotin Following collection, resuspension in 2 ml of lysis buffer, followed by sonication. Phenol extraction of aliquote followed by storage at -80°C libraries constructed WITHOUT rRNA depletion by 1) fragmentation of RNA 2) first strand synthesis with random primers 3) second strand synthesis with random primers 4) end repair and A tailing 5) adapter ligation 6) size selection and quantification and 7) sequencing

text: Escherichia coli K-12

text: Escherichia coli K-12

text: Escherichia coli K-12

text: MEK28 steady state


E-MTAB-5221:MEK28 valine

characteristic: ENA-CHECKLIST

text: Department of Biology University of Copenhagen

text: public

text: E-MTAB-5221:MEK28 valine

text: ArrayExpress

text: Protocols: Cells harvested right before and 10 min after valine addition. 280 ml of cultures were harvested, centrifuged and washed in 0° C biotin-free medium 10 generations of growth on MOPS minimal medium supplemented with 0.2% glucose and 5 µg/ml biotin Isoleucine starvation introduced by addition of 400 µg/ml valine Following collection, resuspension in 2 ml of lysis buffer, followed by sonication. Phenol extraction of aliquote followed by storage at -80°C libraries constructed WITHOUT rRNA depletion by 1) fragmentation of RNA 2) first strand synthesis with random primers 3) second strand synthesis with random primers 4) end repair and A tailing 5) adapter ligation 6) size selection and quantification and 7) sequencing

text: Escherichia coli K-12

text: Escherichia coli K-12

text: Escherichia coli K-12

text: MEK28 valine


characteristic: ENA-FIRST-PUBLIC

text: LSA-DISTAL-UNIBO

text: public

characteristic: collection date

text: Ceca LAB in drinking water control 0 d

text: chicken

characteristic: environment (biome)

text: ceca content

characteristic: environment (feature)

text: ceca content

characteristic: environment (material)

text: Italy

characteristic: geographic location (country and/or sea)

text: 44°26'4272 N

characteristic: geographic location (latitude)

text: 11°28'3540 E

characteristic: geographic location (longitude)

text: host-associated

characteristic: host-associated environmental package

text: metagenome

characteristic: investigation type

text: metagenome

text: LSA LAB in drinking water

characteristic: project name

text: metagenome

text: shotgun

characteristic: sequencing method


text: male

characteristic: ArrayExpress-Sex

text: Medaka

characteristic: ArrayExpress-Species

text: Inbred

characteristic: ArrayExpress-StrainOrLine

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: Japanese medaka

characteristic: common name

text: Oryzias latipes

characteristic: organism

text: Inbred Medaka fish

characteristic: sample description

text: Oryzias latipes

text: Inbred

characteristic: strain

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: LSA-DISTAL-UNIBO

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: Ceca LAB in drinking water high dose 14 d

characteristic: description

text: chicken

characteristic: environment (biome)

text: ceca content

characteristic: environment (feature)

text: ceca content

characteristic: environment (material)

text: Italy

characteristic: geographic location (country and/or sea)

text: 44°26'4272 N

characteristic: geographic location (latitude)

text: 11°28'3540 E

characteristic: geographic location (longitude)

text: host-associated

characteristic: host-associated environmental package

text: metagenome

text: metagenome

text: LSA LAB in drinking water

text: metagenome

text: shotgun


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Candiolo Cancer Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

tag: Namespace:Candiolo Cancer Institute

text: human

characteristic: common name

text: Homo sapiens

characteristic: organism

text: Homo sapiens


E-MTAB-5227:Sample 1

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-5227:Sample 1

tag: Namespace:Chinese Academy of Agricultural Sciences

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: Protocols: collection of larvae and pupa from lab-grown using aphids feeding in the laboratory larva and pupa were two group of organisms for experimental analysis of outcome,three replicates each group extracted RNA from the sample and sequenced

text: 3 days larva

characteristic: developmental stage

text: Lysiphlebia japonica

text: Lysiphlebia japonica

text: whole organism

characteristic: organism part

text: Lysiphlebia japonica

text: mixed sex

characteristic: sex

text: frozen specimen

characteristic: specimen with known storage state

text: Ashmeab

characteristic: strain

text: Sample 1

characteristic: title


E-MTAB-5227:Sample 2

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Chinese Academy of Agricultural Sciences

characteristic: INSDC center name

text: public

text: E-MTAB-5227:Sample 2

text: ArrayExpress

characteristic: description

text: 3 days larva

text: Lysiphlebia japonica

text: Lysiphlebia japonica

text: whole organism

text: Lysiphlebia japonica

text: mixed sex

text: frozen specimen

text: Ashmeab

text: Sample 2


E-MTAB-5227:Sample 3

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-5227:Sample 3

text: ArrayExpress

text: 3 days larva

text: Lysiphlebia japonica

characteristic: organism

text: Lysiphlebia japonica

text: whole organism

text: Lysiphlebia japonica

text: mixed sex

text: frozen specimen

text: Ashmeab

text: Sample 3


E-MTAB-5227:Sample 4

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: INSDC status

text: E-MTAB-5227:Sample 4

text: ArrayExpress

text: pupa

text: Lysiphlebia japonica

text: Lysiphlebia japonica

text: whole organism

text: Lysiphlebia japonica

text: mixed sex

text: frozen specimen

text: Ashmeab

text: Sample 4


E-MTAB-5227:Sample 5

text: public

characteristic: SRA accession

text: E-MTAB-5227:Sample 5

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: pupa

text: Lysiphlebia japonica

text: Lysiphlebia japonica

text: whole organism

text: Lysiphlebia japonica

text: mixed sex

text: frozen specimen

text: Ashmeab

text: Sample 5


E-MTAB-5227:Sample 6

text: public

text: E-MTAB-5227:Sample 6

text: ArrayExpress

text: pupa

characteristic: developmental stage

text: Lysiphlebia japonica

text: Lysiphlebia japonica

text: whole organism

characteristic: organism part

text: Lysiphlebia japonica

text: mixed sex

characteristic: sex

text: frozen specimen

text: Ashmeab

text: Sample 6


E-MTAB-5228:parasitized aphids-1

text: public

text: E-MTAB-5228:parasitized aphids-1

text: ArrayExpress

text: cotton aphid

characteristic: common name

text: Protocols: collection of parasitized aphids (throw away the wasp larva) and unparasitized aphids from lab-grown aphids come from same mother were feeded in the laboratory 3 days parasitized phids and unparasitized phids two group of organisms for experimental analysis of outcome,three replicates each group extracted RNA from the sample and sequenced

text: parasitized by Lysiphlebia japonica

characteristic: disease

text: Aphis gossypii

text: Aphis gossypii

text: whole organism

text: Aphis gossypii

text: mixed sex

text: frozen specimen

characteristic: specimen with known storage state

text: Glover

characteristic: strain

text: parasitized aphids-1

characteristic: title


E-MTAB-5228:parasitized aphids-2

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-5228:parasitized aphids-2

text: ArrayExpress

text: cotton aphid

characteristic: description

text: parasitized by Lysiphlebia japonica

text: Aphis gossypii

text: Aphis gossypii

text: whole organism

text: Aphis gossypii

text: mixed sex

text: frozen specimen

text: Glover

text: parasitized aphids-2


E-MTAB-5228:parasitized aphids-3

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-5228:parasitized aphids-3

text: ArrayExpress

text: cotton aphid

text: parasitized by Lysiphlebia japonica

text: Aphis gossypii

characteristic: organism

text: Aphis gossypii

text: whole organism

text: Aphis gossypii

text: mixed sex

text: frozen specimen

text: Glover

text: parasitized aphids-3


E-MTAB-5228:unparasitized aphids-1

text: public

characteristic: SRA accession

text: E-MTAB-5228:unparasitized aphids-1

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: cotton aphid

text: normal

text: Aphis gossypii

text: Aphis gossypii

text: whole organism

characteristic: organism part

text: Aphis gossypii

text: mixed sex

text: frozen specimen

text: Glover

text: unparasitized aphids-1


E-MTAB-5228:unparasitized aphids-2

text: public

text: E-MTAB-5228:unparasitized aphids-2

text: ArrayExpress

text: cotton aphid

characteristic: common name

text: normal

characteristic: disease

text: Aphis gossypii

text: Aphis gossypii

text: whole organism

text: Aphis gossypii

text: mixed sex

characteristic: sex

text: frozen specimen

characteristic: specimen with known storage state

text: Glover

text: unparasitized aphids-2


E-MTAB-5228:unparasitized aphids-3

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-5228:unparasitized aphids-3

text: ArrayExpress

text: cotton aphid

text: normal

text: Aphis gossypii

text: Aphis gossypii

text: whole organism

text: Aphis gossypii

text: mixed sex

text: frozen specimen

text: Glover

characteristic: strain

text: unparasitized aphids-3

characteristic: title


CSR gamma1 WT spleno J0 [Np-KLH]

characteristic: ENA-CHECKLIST

text: UNIVERSITE DE LIMOGES

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: CSR gamma1 WT spleno J0 [Np-KLH]

tag: Namespace:UNIVERSITE DE LIMOGES

text: house mouse

text: Mus musculus

text: Mus musculus

text: CSReport test datasets


CSR gamma1 WT spleno J0 [Np-Ficoll]

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITE DE LIMOGES

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: CSR gamma1 WT spleno J0 [Np-Ficoll]

text: house mouse

text: Mus musculus

text: Mus musculus

text: CSReport test datasets


name: CSR gamma1 WT spleno J3 [Np-KLH] LPS+IL-4

text: UNIVERSITE DE LIMOGES

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: house mouse

text: Mus musculus

characteristic: organism

text: Mus musculus

text: CSReport test datasets


name: CSR gamma1 WT spleno J3 [Np-Ficoll] LPS+IL-4

text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

characteristic: common name

text: Mus musculus

text: Mus musculus

text: CSReport test datasets


Aa_OM 1

text: public

text: Aa_OM 1

tag: Namespace:MAX PLANCK INSTITUTE FOR PLANT BREEDING RESEARCH

text: gray rockcress

text: Arabis alpina genomic DNA sample for producing BioNano Optical Mapping data

characteristic: description

text: Arabis alpina

text: Arabis alpina

text: Arabis alpina DNA for BioNano Optical Mapping


text: MAX PLANCK INSTITUTE FOR PLANT BREEDING RESEARCH

text: public

text: Conringia planisiliqua whole genomic DNA sample for producing BioNano Optical Mapping data

text: Conringia planisiliqua

text: Conringia planisiliqua

text: Conringia planisiliqua DNA for BioNano Optical Mapping


text: public

text: Euclidium syriacum whole genomic DNA sample for producing BioNano Optical Mapping data

text: Euclidium syriacum

text: Euclidium syriacum

text: Euclidium syriacum DNA for BioNano Optical Mapping


text: public

text: gray rockcress

text: Arabis alpina whole genomic DNA sample for Dovetail Hi-C sequencing

text: Arabis alpina

text: Arabis alpina


name: CSR alpha CH12 aliq sophie controle J+3 A

text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mus musculus

text: Mus musculus

text: CSReport test datasets


name: CSR alpha CH12 aliq sophie controle J+3 B

text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mus musculus

text: Mus musculus

text: CSReport test datasets


name: CSR alpha CH12 aliq sophie controle J+3 C

text: UNIVERSITE DE LIMOGES

text: public

text: house mouse

text: Mus musculus

text: Mus musculus

text: CSReport test datasets


text: DKFZ

text: public

characteristic: cell line

text: human

text: Replicate 1 of exosome ncRNA enriched fraction

text: Homo sapiens

text: Homo sapiens


text: DKFZ

text: public

text: human

text: Replicate 2 of exosome ncRNA enriched fraction

text: Homo sapiens

text: Homo sapiens


text: DKFZ

text: public

text: human

text: Replicate 1 of PC3 cellular ncRNA fraction

text: Homo sapiens

text: Homo sapiens


text: DKFZ

text: public

text: human

text: Replicate 2 of PC3 cellular ncRNA fraction

text: Homo sapiens

text: Homo sapiens


text: LSA-DISTAL-UNIBO

text: public

characteristic: collection date

text: chicken

characteristic: environment (biome)

text: ceca content

characteristic: environment (feature)

text: ceca content

characteristic: environment (material)

text: Italy

characteristic: geographic location (country and/or sea)

text: 44°26'4272 N

characteristic: geographic location (latitude)

text: 11°28'3540 E

characteristic: geographic location (longitude)

text: host-associated

characteristic: host-associated environmental package

text: metagenome

characteristic: investigation type

text: metagenome

text: LSA LAB in drinking water

characteristic: project name

text: metagenome

text: shotgun

characteristic: sequencing method


text: Gut Bacterium

characteristic: ArrayExpress-Phenotype

text: Clostridium difficile

characteristic: ArrayExpress-Species

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Clostridium difficile

text: Clostridioides difficile

text: Bacterial Genome

characteristic: sample description

text: Clostridioides difficile

text: Clostridium difficile

characteristic: title


text: Institute of Food Research

text: public

tag: Namespace:Institute  of Food Research

characteristic: Submitter Id

characteristic: collection date

text: ELBW infant no supplementation

characteristic: description

text: gut

characteristic: environment (biome)

text: gut

characteristic: environment (feature)

text: feces

characteristic: environment (material)

text: United Kingdom

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: human-gut

characteristic: human gut environmental package

text: metagenome

text: feces metagenome

characteristic: organism

text: ELBW gut microbiome

text: feces metagenome

text: 16S rRNA gene sequencing

text: ELBW infant no supplementation


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Institute of Food Research

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ELBW infant with supplementation

text: gut

text: gut

text: feces

text: United Kingdom

text: human-gut

text: metagenome

characteristic: investigation type

text: feces metagenome

text: ELBW gut microbiome

characteristic: project name

text: feces metagenome

text: 16S rRNA gene sequencing

characteristic: sequencing method

text: ELBW infant with supplementation


text: Institute of Food Research

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: term baby

characteristic: description

text: gut

characteristic: environment (biome)

text: gut

text: feces

text: United Kingdom

text: human-gut

text: metagenome

text: feces metagenome

text: ELBW gut microbiome

text: feces metagenome

text: 16S rRNA gene sequencing

text: term baby

characteristic: title


characteristic: ENA-CHECKLIST

text: UNIVERSITY OF CAMBRIDGE

text: public

tag: Namespace:UNIVERSITY OF CAMBRIDGE

text: Boreal

text: Lake

characteristic: environment (feature)

text: Sediment

characteristic: environment (material)

text: Canada

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: o2 consumption

text: freshwater sediment metagenome

characteristic: organism

text: Daisy Lake Shotgun

characteristic: respiration rate

text: freshwater sediment metagenome

text: sediment

characteristic: sediment environmental package

text: Illumina

text: Daisy Lake Shotgun


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INDIANA UNIVERSITY BLOOMINGTON

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Daphnia obtusa

text: Daphnia obtusa

text: daphnia obtusa


E-MTAB-5229:Sample 1

characteristic: ENA-CHECKLIST

text: Wellcome Trust Sanger Institute

text: public

text: E-MTAB-5229:Sample 1

tag: Namespace:Wellcome Trust Sanger Institute

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: embryonic stem cell

characteristic: cell type

text: human

characteristic: common name

text: Protocols: m6A MeRIP on nuclear-enriched RNA (NeMeRIP) were performed following modifications of previously described methods (Dominissini et al. 2013; Batista et al. 2014). 7.5E07 hESCs were used for each sample, and three biological replicates per condition were generated. Cells were fed with fresh medium for 2h before being washed with PBS, scraped in cell dissociation buffer (CDB, Gibco), and pelleted at 250g for 5’. The cell pellet was incubated in five volumes of isotonic lysis buffer (ILB: 10mM Tris-HCl pH 7.5; 3mM CaCl2; 2mM MgCl2; 0.32M sucrose; 1,000U/ml RNAsin ribonuclease inhibitor, Promega; and 1mM DTT) for 10’ to induce cell swelling. Then, Triton X-100 was added to a final concentration of 0.3% and cells were incubated for 6’ to lyse the plasma membranes. Nuclei were pelleted at 600g for 5’, washed once with ten volumes of ILB. Feeder- and serum-free hESC (H9 line; WiCell) and hiPSC (A1ATR/R line, Yusa et al., 2011) culture was previously described (Vallier, 2011). Cells were plated on gelatin- and MEF medium-coated plates, and cultured in chemically defined medium (CDM) containing bovine serum albumin (BSA). CDM was supplemented with 10ng/ml Activin-A and 12ng/ml FGF2 (both from Dr Marko Hyvonen, Dept. of Biochemistry, University of Cambridge). Cells were fed with fresh media containing 10ng/ml Activin-A or 10uM SB-431542. Samples were collected after 2h. RNA was extracted from the nuclear pellet using the RNeasy midi kit (QIAGEN) according to manufacturer’s instructions. Residual contaminating DNA was digested in solution using the RNAse-free DNase Set from QIAGEN, and RNA was re-purified by sequential acid phenol-chloroform and chloroform extractions followed by ethanol precipitation. At this stage, complete removal of DNA contamination was confirmed by qPCR of the resulting RNA without a retrotranscription step. RNA was then chemically fragmented in 20μl reactions each containing 20μg of RNA in fragmentation buffer (FB: 10mM ZnCl2; 10mM Tris-HCl pH 7.0). Such reactions were incubated at 95°C for 5’, followed by inactivation with 50mM EDTA and storage on ice. The fragmented RNA was then cleaned up by ethanol precipitation. In preparation to the MeRIP, 15μg of anti m6A-antibody (Synaptic Systems, catalogue number: 202-003) or equivalent amounts of rabbit non-immune IgG were cross-linked to 0.5mg of magnetic beads by using the Dynabeads Antibody Coupling Kit (ThermoFisher Scientific) according to manufacturer’s instructions. Following equilibration of the magnetic beads by washing with 500μl of binding buffer (BB: 50mM Tris-HCl pH 7.5; 150mM NaCl2; 1% NP-40; 1mM EDTA. MeRIP reactions were assembled with 300μg of the fragmented RNA in 3m of BB supplemented with 500U of RNAsin ribonuclease inhibitor. Samples were incubated at 7rpm for 1h at RT. 5μg of fragmented RNA (10% of the amount used for MeRIP) were set aside as pre-MeRIP input control. MeRIP reactions were washed twice with BB, once with low-salt buffer [LSB: 0.25x SSPE (saline-sodium phosphate-EDTA buffer: 150mM NaCl; 10mM NaHPO4-H2O; 10mM Na2-EDTA; @ pH 7.4); 37.5mM NaCl2; 1mM EDTA; 0.05% Tween-20), once with high-salt buffer (HSB: 0.25x SSPE; 137.5mM NaCl2; 1mM EDTA; 0.05% Tween-20), and twice with TE-Tween buffer (TTB: 10mM Tris-HCl pH 7.4; 1mM EDTA; 0.05% Tween-20). Each wash performed by incubating the beads with 500μl of buffer at 7rpm for 3’ at RT. Finally, RNA was eluted from the beads by four successive incubations with 75μl of elution buffer (EB: 50mM Tris-HCl pH 7.5; 150mM NaCl2; 20mM DTT; 0.1% SDS; 1mM EDTA) at 42°C. Both the RNA from pooled MeRIP eluates and the pre-MeRIP input were purified and concentrated by sequential acid phenol-chloroform and chloroform extractions followed by ethanol precipitation. 30μg of glycogen were added as carrier during ethanol precipitation. RNA was resuspended in 15μl of ultrapure RNAse-free water. Library preparation was performed using the Standard TruSeq total RNA kit (Illumina) according to manufacturer’s instructions with the following exceptions: (1) Ribo-Zero treatment was performed only for pre-MeRIP samples, as ribosomal RNA contamination in m6A MeRIP samples was minimal; (1) since samples were pre-fragmented, the fragmentation step was bypassed and 30ng of RNA for each sample were used directly for library prep; (3) due to the small size of the library, a 2-fold excess of Ampure XP beads was used during all purification steps in order to retain small fragments; (4) due to the presence of contaminating adapter dimers, the library was gel extracted using gel safe stain and a dark reader in order to remove fragments smaller than ~120bp.

characteristic: description

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: female

characteristic: sex

text: Sample 1

characteristic: title


E-MTAB-5229:Sample 2

characteristic: ENA-FIRST-PUBLIC

text: Wellcome Trust Sanger Institute

text: public

text: E-MTAB-5229:Sample 2

text: ArrayExpress

text: embryonic stem cell

text: human

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Homo sapiens

text: female

text: Sample 2


E-MTAB-5229:Sample 3

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Wellcome Trust Sanger Institute

text: public

text: E-MTAB-5229:Sample 3

text: ArrayExpress

text: embryonic stem cell

text: human

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: female

text: Sample 3


E-MTAB-5229:Sample 7

text: Wellcome Trust Sanger Institute

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-5229:Sample 7

text: ArrayExpress

text: embryonic stem cell

text: human

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: female

text: Sample 7


E-MTAB-5229:Sample 8

text: Wellcome Trust Sanger Institute

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-5229:Sample 8

text: ArrayExpress

text: embryonic stem cell

text: human

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: female

text: Sample 8


E-MTAB-5229:Sample 9

text: Wellcome Trust Sanger Institute

text: public

characteristic: SRA accession

text: E-MTAB-5229:Sample 9

characteristic: Submitter Id

text: ArrayExpress

text: embryonic stem cell

text: human

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: female

text: Sample 9


E-MTAB-5229:Sample 10

characteristic: ENA-CHECKLIST

text: Wellcome Trust Sanger Institute

text: public

text: E-MTAB-5229:Sample 10

text: ArrayExpress

characteristic: broker name

text: embryonic stem cell

text: human

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: female

text: Sample 10


E-MTAB-5229:Sample 11

text: Wellcome Trust Sanger Institute

text: public

text: E-MTAB-5229:Sample 11

text: ArrayExpress

characteristic: cell line

text: embryonic stem cell

characteristic: cell type

text: human

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: female

text: Sample 11


E-MTAB-5229:Sample 12

text: Wellcome Trust Sanger Institute

text: public

text: E-MTAB-5229:Sample 12

text: ArrayExpress

text: embryonic stem cell

text: human

characteristic: common name

characteristic: description

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: female

text: Sample 12


E-MTAB-5229:Sample 4

text: Wellcome Trust Sanger Institute

text: public

text: E-MTAB-5229:Sample 4

text: ArrayExpress

text: embryonic stem cell

text: human

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: female

characteristic: sex

text: Sample 4

characteristic: title


E-MTAB-5229:Sample 5

characteristic: ENA-FIRST-PUBLIC

text: Wellcome Trust Sanger Institute

text: public

text: E-MTAB-5229:Sample 5

text: ArrayExpress

text: embryonic stem cell

text: human

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Homo sapiens

text: female

text: Sample 5


E-MTAB-5229:Sample 6

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Wellcome Trust Sanger Institute

text: public

text: E-MTAB-5229:Sample 6

text: ArrayExpress

text: embryonic stem cell

text: human

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: female

text: Sample 6


E-MTAB-5230:Sample 1

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-5230:Sample 1

tag: Namespace:Wellcome Trust - Medical Research Council Cambridge Stem Cell Institute, Anne McLaren Laboratory for Regenerative Medicine, Department of Surgery, University of Cambridge

text: ArrayExpress

text: embryonic stem cell

text: human

text: Protocols: Feeder- and serum-free hESC (H9 line; WiCell) culture was previously described (Vallier, 2011). Cells were plated on gelatin- and MEF medium-coated plates, and cultured in chemically defined medium (CDM) containing bovine serum albumin (BSA). CDM was supplemented with 10ng/ml Activin-A and 12ng/ml FGF2 (both from Dr Marko Hyvonen, Dept. of Biochemistry, University of Cambridge). Cells were treated for 2h with fresh culture media containing 10ng/ml Activin-A, or cultured for 2h, 8h, or 32h of with media containing 10uM SB-431542 (SB) instead of Activin-A. Three biological replicates per condition were examined Cellular RNA was extracted using the GenElute Mammalian Total RNA Miniprep Kit and the On-Column DNase I Digestion Set (both from Sigma-Aldrich) following manufacturer’s instructions. Polyadenylated (poly-A) purified opposing strand-specific mRNA library libraries were prepared from 200ng of total RNA using the TruSeq Stranded mRNA HT sample preparation kit (Illumina). Samples were individually indexed for pooling using a dual-index strategy. Libraries were quantified both with a Qubit (ThermoFisher Scientific) and by qPCR using the NGS Library. Quantification Kit (KAPA Biosystems). Libraries were then normalized and pooled.

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: female

text: Sample 1


E-MTAB-5230:Sample 2

text: Wellcome Trust - Medical Research Council Cambridge Stem Cell Institute, Anne McLaren Laboratory for Regenerative Medicine, Department of Surgery, University of Cambridge

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-5230:Sample 2

text: ArrayExpress

text: embryonic stem cell

text: human

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: female

text: Sample 2


E-MTAB-5230:Sample 3

text: public

characteristic: SRA accession

text: E-MTAB-5230:Sample 3

characteristic: Submitter Id

text: ArrayExpress

text: embryonic stem cell

text: human

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: female

text: Sample 3


E-MTAB-5230:Sample 4

characteristic: ENA-CHECKLIST

text: public

text: E-MTAB-5230:Sample 4

text: ArrayExpress

characteristic: broker name

text: embryonic stem cell

text: human

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: female

text: Sample 4


E-MTAB-5230:Sample 5

text: public

text: E-MTAB-5230:Sample 5

text: ArrayExpress

characteristic: cell line

text: embryonic stem cell

characteristic: cell type

text: human

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: female

text: Sample 5


E-MTAB-5230:Sample 6

text: public

text: E-MTAB-5230:Sample 6

text: ArrayExpress

text: embryonic stem cell

text: human

characteristic: common name

characteristic: description

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: female

text: Sample 6


E-MTAB-5230:Sample 7

text: public

text: E-MTAB-5230:Sample 7

text: ArrayExpress

text: embryonic stem cell

text: human

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: female

characteristic: sex

text: Sample 7

characteristic: title


E-MTAB-5230:Sample 8

characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-5230:Sample 8

text: ArrayExpress

text: embryonic stem cell

text: human

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Homo sapiens

text: female

text: Sample 8


E-MTAB-5230:Sample 9

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-5230:Sample 9

text: ArrayExpress

text: embryonic stem cell

text: human

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: female

text: Sample 9


E-MTAB-5230:Sample 10

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-5230:Sample 10

text: ArrayExpress

text: embryonic stem cell

text: human

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: female

text: Sample 10


E-MTAB-5230:Sample 11

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-5230:Sample 11

text: ArrayExpress

text: embryonic stem cell

text: human

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: female

text: Sample 11


E-MTAB-5230:Sample 12

text: public

characteristic: SRA accession

text: E-MTAB-5230:Sample 12

characteristic: Submitter Id

text: ArrayExpress

text: embryonic stem cell

text: human

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: female

text: Sample 12


E-MTAB-5230:Sample 13

characteristic: ENA-CHECKLIST

text: public

text: E-MTAB-5230:Sample 13

text: ArrayExpress

characteristic: broker name

text: embryonic stem cell

text: human

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: female

text: Sample 13


E-MTAB-5230:Sample 14

text: public

text: E-MTAB-5230:Sample 14

text: ArrayExpress

characteristic: cell line

text: embryonic stem cell

characteristic: cell type

text: human

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: female

text: Sample 14


E-MTAB-5230:Sample 15

text: public

text: E-MTAB-5230:Sample 15

text: ArrayExpress

text: embryonic stem cell

text: human

characteristic: common name

characteristic: description

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: female

text: Sample 15


E-MTAB-5230:Sample 16

text: public

text: E-MTAB-5230:Sample 16

text: ArrayExpress

text: embryonic stem cell

text: human

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: female

characteristic: sex

text: Sample 16

characteristic: title


E-MTAB-5230:Sample 17

characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-5230:Sample 17

text: ArrayExpress

text: embryonic stem cell

text: human

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Homo sapiens

text: female

text: Sample 17


E-MTAB-5230:Sample 18

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-5230:Sample 18

text: ArrayExpress

text: embryonic stem cell

text: human

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: female

text: Sample 18


text: Univeristy of Aberdeen

characteristic: INSDC center name

characteristic: INSDC first public

text: public

tag: Namespace:Univeristy of Aberdeen

characteristic: collection date

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: diseased

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

characteristic: isolate

text: Biopsy

characteristic: isolation source

text: Campylobacter showae

text: Campylobacter showae

characteristic: strain

text: Campylobacter showae strain from a biopsy of a Paediatric patient with Crohn's disease


characteristic: ENA-CHECKLIST

text: Univeristy of Aberdeen

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: United Kingdom

text: diseased

text: Homo sapiens

text: Biopsy

text: Campylobacter showae

text: Campylobacter showae

text: Campylobacter showae strain from a biopsy of an Adult patient with a colonic Adenoma


characteristic: ENA-FIRST-PUBLIC

text: Univeristy of Aberdeen

text: public

text: United Kingdom

text: diseased

text: Homo sapiens

text: CAM

text: Stool

text: Campylobacter showae

characteristic: organism

text: Campylobacter showae

text: CAM

text: Campylobacter showae strain from a stool sample of an Adult patient with gastroenteritis

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

tag: Namespace:UNIVERSITY OF SHEFFIELD, DEPARTMENT OF ANIMAL & PL

text: Growth chamber study

characteristic: environment (biome)

text: Growth chamber study

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: United Kingdom

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: metagenome

text: Rhizosphere chemistry and bacterial communities

characteristic: project name

text: metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: Root and associated soil sample 1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UNIVERSITY OF SHEFFIELD, DEPARTMENT OF ANIMAL & PL

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: Growth chamber study

text: Growth chamber study

text: soil

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: metagenome

text: metagenome

text: metagenome

text: Illumina MiSeq

text: soil

text: Root and associated soil sample 2


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

text: public

text: Growth chamber study

characteristic: environment (biome)

text: Growth chamber study

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: United Kingdom

characteristic: geographic location (depth)

text: metagenome

text: metagenome

characteristic: organism

text: metagenome

text: Illumina MiSeq

text: soil

text: Root and associated soil sample 3

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: Growth chamber study

text: Growth chamber study

text: soil

text: United Kingdom

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: metagenome

characteristic: project name

text: metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: Root and associated soil sample 4


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: Growth chamber study

text: Growth chamber study

text: soil

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: metagenome

text: metagenome

text: metagenome

text: Illumina MiSeq

text: soil

text: Control soil sample 1


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC center name

text: public

text: Growth chamber study

characteristic: environment (biome)

text: Growth chamber study

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: United Kingdom

characteristic: geographic location (depth)

text: metagenome

text: metagenome

characteristic: organism

text: metagenome

text: Illumina MiSeq

text: soil

text: Control soil sample 2

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: Growth chamber study

text: Growth chamber study

text: soil

text: United Kingdom

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: metagenome

characteristic: project name

text: metagenome

text: Illumina MiSeq

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: Control soil sample 3


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: Growth chamber study

text: Growth chamber study

text: soil

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: metagenome

text: metagenome

text: metagenome

text: Illumina MiSeq

text: soil

text: Control soil sample 4


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: JUSTUS LIEBIG UNIVERSITY

characteristic: INSDC center name

text: public

tag: Namespace:JUSTUS LIEBIG UNIVERSITY

text: Listeria sp. ILCC801

characteristic: organism

text: Listeria sp. ILCC801

text: Listeria goanesis ILCC792

characteristic: title


tag: attribute

text: ESE

text: public

text: Microbotryum silenes-dioicae (A1)

characteristic: description

characteristic: mating type

text: Microbotryum silenes-dioicae

text: Microbotryum silenes-dioicae

text: Microbotryum silenes-dioicae (A1)


text: ESE

text: public

text: Microbotryum silenes-dioicae (A2)

text: Microbotryum silenes-dioicae

text: Microbotryum silenes-dioicae

text: Microbotryum silenes-dioicae (A2)


text: JUSTUS LIEBIG UNIVERSITY

text: public

text: Listeria sp. ILCC801

text: Listeria sp. ILCC801

text: Listeria goanesis ILCC796


text: JUSTUS LIEBIG UNIVERSITY

text: public

text: Listeria sp. ILCC801

text: Listeria sp. ILCC801

text: Listeria goanesis ILCC797


text: ZFS:0000038 - Larval : Day 6

characteristic: ArrayExpress-DevelopmentalStage

text: Whole embryo

characteristic: ArrayExpress-OrganismPart

text: Danio rerio

characteristic: ArrayExpress-Species

text: mixed

characteristic: ArrayExpress-StrainOrLine

text: SC

text: public

text: zebrafish

characteristic: common name

text: Danio rerio

text: Total RNA from a single zebrafish embryo heterozygous for slit3, allele sa1569 plus ERCC spike mix 1 (Ambion).

characteristic: sample description

text: Danio rerio

text: mixed

characteristic: strain


text: ZFS:0000038 - Larval : Day 6

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: SC

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: zebrafish

text: Danio rerio

text: Total RNA from a single zebrafish embryo homozygous for slit3, allele sa1569 plus ERCC spike mix 1 (Ambion).

text: Danio rerio

text: mixed


text: ZFS:0000038 - Larval : Day 6

text: Whole embryo

text: Danio rerio

text: mixed

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

characteristic: INSDC center name

text: public

text: zebrafish

text: Danio rerio

characteristic: organism

text: Total RNA from a single zebrafish embryo wild type for slit3, allele sa1569 plus ERCC spike mix 1 (Ambion).

text: Danio rerio

text: mixed

characteristic: title


text: JUSTUS LIEBIG UNIVERSITY

text: public

text: Listeria sp. ILCC801

text: Listeria sp. ILCC801

text: Listeria goanesis ILCC804


text: JUSTUS LIEBIG UNIVERSITY

text: public

text: Listeria sp. ILCC801

text: Listeria sp. ILCC801

text: Listeria goanesis ILCC806


text: JUSTUS LIEBIG UNIVERSITY

text: public

text: Listeria sp. ILCC801

text: Listeria sp. ILCC801

text: Listeria goanesis ILCC810


text: Institute of Food Research

text: public

characteristic: collection date

text: preterm no supplementation

characteristic: description

text: gut

characteristic: environment (biome)

text: gut

characteristic: environment (feature)

text: feces

characteristic: environment (material)

text: United Kingdom

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: human-gut

characteristic: human gut environmental package

text: metagenome

characteristic: investigation type

text: feces metagenome

text: Extremelly low birth gut microbiome

characteristic: project name

text: feces metagenome

text: 16S rRNA gene sequencing

characteristic: sequencing method

text: preterm no supplementation


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Institute of Food Research

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: preterm supplementation

text: gut

text: gut

text: feces

text: United Kingdom

text: human-gut

text: metagenome

text: feces metagenome

text: Extremelly low birth gut microbiome

text: feces metagenome

text: 16S rRNA gene sequencing

text: preterm with supplementation


characteristic: External Id

text: Institute of Food Research

characteristic: INSDC center name

text: public

characteristic: collection date

text: preterm supplementation

characteristic: description

text: gut

characteristic: environment (biome)

text: gut

characteristic: environment (feature)

text: feces

text: United Kingdom

text: human-gut

text: metagenome

text: feces metagenome

characteristic: organism

text: Extremelly low birth gut microbiome

text: feces metagenome

text: 16S rRNA gene sequencing

text: preterm supplementation

characteristic: title


text: Institute of Food Research

text: public

text: term baby

text: gut

text: gut

text: feces

characteristic: environment (material)

text: United Kingdom

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: human-gut

characteristic: human gut environmental package

text: metagenome

characteristic: investigation type

text: feces metagenome

text: Extremelly low birth gut microbiome

characteristic: project name

text: feces metagenome

text: 16S rRNA gene sequencing

characteristic: sequencing method

text: term baby


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: SMU

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: averagelengthandsd

text: feces metagenome

characteristic: common name

characteristic: connectratio(%)

characteristic: connecttagnumber

text: feces metagenome

text: feces metagenome

characteristic: tagswithoutprimer

characteristic: tagutilizationratio(%)