E-MTAB-5208:Plate 7 WT E9.5 24

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: The Francis Crick Institute

text: public

text: E-MTAB-5208:Plate 7 WT E9.5 24

text: ArrayExpress

text: primary cell

text: epiblast cell

text: house mouse

text: embryonic day 9.5

text: wild type genotype

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: caudal lateral epiblast

text: Mus musculus

text: Plate 7 WT E9.5 24


E-MTAB-5208:Plate 7 WT E9.5 25

status: PUBLIC

tag: attribute

text: The Francis Crick Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-5208:Plate 7 WT E9.5 25

tag: Namespace:The Francis Crick Institute

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: primary cell

text: epiblast cell

text: house mouse

characteristic: common name

text: Protocols: The caudal lateral epiblast region (L1-L2) was manually micro-dissected from e8.5 and e9.5 wildtype, outbred MF1 mouse embryos. the dissected regions were pooled separately and dissociated into single cells using a combination of enzyme (0.05% trypsin) and mechanical (mouth pipetting) dissociation. The mouse ESC lines, HM1 (wild type) and Tbx6-/- were used for the in vitro differentiation experiments. Briefly, cells were plated on gelatinised dishes in N2B27 medium with bFGF. At day 2 of differentiation the medium was replaced with N2B27 plus 5uM CHIR and 10ng/ml bFGF. At day3 the medium was changed to N2B27 for 24hours. Cells were collected for single cell analysis at D2, D3 (NMPs) and D4 of differentiation. Similarly, Tbx6 mutant cells were differentiated to NMPs. At day3 of differentiation Tbx6-/- cells were passaged in N2B27 plus CHIR/bFGF and cultured for another 3 days. Samples were collected at day3 and day6 of differentiation for single cell analysis. The RNA-Seq libraries were prepared using the Nextera XT DNA library prep kit (Illumina) according to manufacturers instructions described in the protocol (PN 100-7168, http://www. uidigm.com/).

characteristic: description

text: embryonic day 9.5

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: dissected from the caudal lateral epiblast

characteristic: growth condition

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: caudal lateral epiblast

characteristic: organism part

text: Mus musculus

characteristic: strain

text: Plate 7 WT E9.5 25

characteristic: title

type: has member


E-MTAB-5208:Plate 7 WT E9.5 26

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: The Francis Crick Institute

text: public

text: E-MTAB-5208:Plate 7 WT E9.5 26

text: ArrayExpress

text: primary cell

text: epiblast cell

text: house mouse

text: embryonic day 9.5

text: wild type genotype

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: caudal lateral epiblast

text: Mus musculus

text: Plate 7 WT E9.5 26


E-MTAB-5208:Plate 7 WT E9.5 27

text: The Francis Crick Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-5208:Plate 7 WT E9.5 27

text: ArrayExpress

text: primary cell

text: epiblast cell

text: house mouse

text: embryonic day 9.5

text: wild type genotype

text: not applicable

text: Mus musculus

text: Mus musculus

text: caudal lateral epiblast

text: Mus musculus

text: Plate 7 WT E9.5 27


E-MTAB-5208:Plate 7 WT E9.5 28

text: The Francis Crick Institute

text: public

text: E-MTAB-5208:Plate 7 WT E9.5 28

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: primary cell

text: epiblast cell

text: house mouse

characteristic: common name

characteristic: description

text: embryonic day 9.5

characteristic: developmental stage

text: wild type genotype

text: not applicable

text: Mus musculus

text: Mus musculus

text: caudal lateral epiblast

text: Mus musculus

text: Plate 7 WT E9.5 28


E-MTAB-5208:Plate 7 WT E9.5 29

text: The Francis Crick Institute

text: public

text: E-MTAB-5208:Plate 7 WT E9.5 29

text: ArrayExpress

text: primary cell

text: epiblast cell

text: house mouse

text: embryonic day 9.5

text: wild type genotype

characteristic: genotype

characteristic: growth condition

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: caudal lateral epiblast

characteristic: organism part

text: Mus musculus

characteristic: strain

text: Plate 7 WT E9.5 29


E-MTAB-5208:Plate 7 WT E9.5 3

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: The Francis Crick Institute

text: public

text: E-MTAB-5208:Plate 7 WT E9.5 3

text: ArrayExpress

text: primary cell

text: epiblast cell

text: house mouse

text: embryonic day 9.5

text: wild type genotype

text: not applicable

text: Mus musculus

text: Mus musculus

text: caudal lateral epiblast

text: Mus musculus

text: Plate 7 WT E9.5 3

characteristic: title


E-MTAB-5208:Plate 7 WT E9.5 30

text: The Francis Crick Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-5208:Plate 7 WT E9.5 30

text: ArrayExpress

text: primary cell

text: epiblast cell

text: house mouse

text: embryonic day 9.5

text: wild type genotype

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: caudal lateral epiblast

text: Mus musculus

text: Plate 7 WT E9.5 30


E-MTAB-5208:Plate 7 WT E9.5 31

text: The Francis Crick Institute

text: public

characteristic: SRA accession

text: E-MTAB-5208:Plate 7 WT E9.5 31

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: primary cell

text: epiblast cell

text: house mouse

characteristic: common name

characteristic: description

text: embryonic day 9.5

text: wild type genotype

text: not applicable

text: Mus musculus

text: Mus musculus

text: caudal lateral epiblast

text: Mus musculus

text: Plate 7 WT E9.5 31


E-MTAB-5208:Plate 7 WT E9.5 32

text: The Francis Crick Institute

text: public

text: E-MTAB-5208:Plate 7 WT E9.5 32

text: ArrayExpress

text: primary cell

text: epiblast cell

text: house mouse

text: embryonic day 9.5

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

characteristic: growth condition

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: caudal lateral epiblast

characteristic: organism part

text: Mus musculus

text: Plate 7 WT E9.5 32


E-MTAB-5208:Plate 7 WT E9.5 33

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: The Francis Crick Institute

text: public

text: E-MTAB-5208:Plate 7 WT E9.5 33

text: ArrayExpress

text: primary cell

text: epiblast cell

text: house mouse

text: embryonic day 9.5

text: wild type genotype

text: not applicable

text: Mus musculus

text: Mus musculus

text: caudal lateral epiblast

text: Mus musculus

characteristic: strain

text: Plate 7 WT E9.5 33


E-MTAB-5208:Plate 7 WT E9.5 34

text: The Francis Crick Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-5208:Plate 7 WT E9.5 34

text: ArrayExpress

text: primary cell

text: epiblast cell

text: house mouse

text: embryonic day 9.5

text: wild type genotype

text: not applicable

text: Mus musculus

text: Mus musculus

text: caudal lateral epiblast

text: Mus musculus

text: Plate 7 WT E9.5 34

characteristic: title


E-MTAB-5208:Plate 7 WT E9.5 35

text: The Francis Crick Institute

text: public

characteristic: SRA accession

text: E-MTAB-5208:Plate 7 WT E9.5 35

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: primary cell

text: epiblast cell

text: house mouse

characteristic: common name

text: embryonic day 9.5

text: wild type genotype

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: caudal lateral epiblast

text: Mus musculus

text: Plate 7 WT E9.5 35


E-MTAB-5208:Plate 7 WT E9.5 36

text: The Francis Crick Institute

text: public

text: E-MTAB-5208:Plate 7 WT E9.5 36

text: ArrayExpress

text: primary cell

text: epiblast cell

text: house mouse

characteristic: description

text: embryonic day 9.5

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

characteristic: growth condition

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: caudal lateral epiblast

text: Mus musculus

text: Plate 7 WT E9.5 36


E-MTAB-5208:Plate 7 WT E9.5 37

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: The Francis Crick Institute

text: public

text: E-MTAB-5208:Plate 7 WT E9.5 37

text: ArrayExpress

text: primary cell

text: epiblast cell

text: house mouse

text: embryonic day 9.5

text: wild type genotype

text: not applicable

text: Mus musculus

text: Mus musculus

text: caudal lateral epiblast

characteristic: organism part

text: Mus musculus

text: Plate 7 WT E9.5 37


E-MTAB-5208:Plate 7 WT E9.5 38

text: The Francis Crick Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-5208:Plate 7 WT E9.5 38

text: ArrayExpress

text: primary cell

text: epiblast cell

text: house mouse

text: embryonic day 9.5

text: wild type genotype

text: not applicable

text: Mus musculus

text: Mus musculus

text: caudal lateral epiblast

text: Mus musculus

characteristic: strain

text: Plate 7 WT E9.5 38


E-MTAB-5208:Plate 7 WT E9.5 39

text: The Francis Crick Institute

text: public

characteristic: SRA accession

text: E-MTAB-5208:Plate 7 WT E9.5 39

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: primary cell

text: epiblast cell

text: house mouse

characteristic: common name

text: embryonic day 9.5

text: wild type genotype

text: not applicable

text: Mus musculus

text: Mus musculus

text: caudal lateral epiblast

text: Mus musculus

text: Plate 7 WT E9.5 39

characteristic: title


E-MTAB-5208:Plate 7 WT E9.5 4

text: The Francis Crick Institute

text: public

text: E-MTAB-5208:Plate 7 WT E9.5 4

text: ArrayExpress

text: primary cell

text: epiblast cell

text: house mouse

characteristic: description

text: embryonic day 9.5

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

characteristic: growth condition

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: caudal lateral epiblast

text: Mus musculus

text: Plate 7 WT E9.5 4


E-MTAB-5208:Plate 7 WT E9.5 40

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: The Francis Crick Institute

text: public

text: E-MTAB-5208:Plate 7 WT E9.5 40

text: ArrayExpress

text: primary cell

text: epiblast cell

text: house mouse

text: embryonic day 9.5

text: wild type genotype

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: caudal lateral epiblast

text: Mus musculus

text: Plate 7 WT E9.5 40


E-MTAB-5208:Plate 7 WT E9.5 41

text: The Francis Crick Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-5208:Plate 7 WT E9.5 41

text: ArrayExpress

text: primary cell

text: epiblast cell

text: house mouse

text: embryonic day 9.5

text: wild type genotype

text: not applicable

text: Mus musculus

text: Mus musculus

text: caudal lateral epiblast

characteristic: organism part

text: Mus musculus

text: Plate 7 WT E9.5 41


E-MTAB-5208:Plate 7 WT E9.5 42

text: The Francis Crick Institute

text: public

characteristic: SRA accession

text: E-MTAB-5208:Plate 7 WT E9.5 42

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: primary cell

text: epiblast cell

text: house mouse

characteristic: common name

text: embryonic day 9.5

text: wild type genotype

text: not applicable

text: Mus musculus

text: Mus musculus

text: caudal lateral epiblast

text: Mus musculus

characteristic: strain

text: Plate 7 WT E9.5 42


E-MTAB-5208:Plate 7 WT E9.5 43

text: The Francis Crick Institute

text: public

text: E-MTAB-5208:Plate 7 WT E9.5 43

text: ArrayExpress

text: primary cell

text: epiblast cell

text: house mouse

characteristic: description

text: embryonic day 9.5

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

characteristic: growth condition

text: not applicable

text: Mus musculus

text: Mus musculus

text: caudal lateral epiblast

text: Mus musculus

text: Plate 7 WT E9.5 43

characteristic: title


E-MTAB-5208:Plate 7 WT E9.5 44

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: The Francis Crick Institute

text: public

text: E-MTAB-5208:Plate 7 WT E9.5 44

text: ArrayExpress

text: primary cell

text: epiblast cell

text: house mouse

text: embryonic day 9.5

text: wild type genotype

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: caudal lateral epiblast

text: Mus musculus

text: Plate 7 WT E9.5 44


E-MTAB-5208:Plate 7 WT E9.5 45

text: The Francis Crick Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-5208:Plate 7 WT E9.5 45

text: ArrayExpress

text: primary cell

text: epiblast cell

text: house mouse

text: embryonic day 9.5

text: wild type genotype

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: caudal lateral epiblast

text: Mus musculus

text: Plate 7 WT E9.5 45


E-MTAB-5208:Plate 7 WT E9.5 46

text: The Francis Crick Institute

text: public

characteristic: SRA accession

text: E-MTAB-5208:Plate 7 WT E9.5 46

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: primary cell

text: epiblast cell

text: house mouse

characteristic: common name

text: embryonic day 9.5

text: wild type genotype

text: not applicable

text: Mus musculus

text: Mus musculus

text: caudal lateral epiblast

characteristic: organism part

text: Mus musculus

text: Plate 7 WT E9.5 46


E-MTAB-5208:Plate 7 WT E9.5 47

text: The Francis Crick Institute

text: public

text: E-MTAB-5208:Plate 7 WT E9.5 47

text: ArrayExpress

text: primary cell

text: epiblast cell

text: house mouse

characteristic: description

text: embryonic day 9.5

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

characteristic: growth condition

text: not applicable

text: Mus musculus

text: Mus musculus

text: caudal lateral epiblast

text: Mus musculus

characteristic: strain

text: Plate 7 WT E9.5 47


E-MTAB-5208:Plate 7 WT E9.5 48

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: The Francis Crick Institute

text: public

text: E-MTAB-5208:Plate 7 WT E9.5 48

text: ArrayExpress

text: primary cell

text: epiblast cell

text: house mouse

text: embryonic day 9.5

text: wild type genotype

text: not applicable

text: Mus musculus

text: Mus musculus

text: caudal lateral epiblast

text: Mus musculus

text: Plate 7 WT E9.5 48

characteristic: title


E-MTAB-5208:Plate 7 WT E9.5 49

text: The Francis Crick Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-5208:Plate 7 WT E9.5 49

text: ArrayExpress

text: primary cell

text: epiblast cell

text: house mouse

text: embryonic day 9.5

text: wild type genotype

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: caudal lateral epiblast

text: Mus musculus

text: Plate 7 WT E9.5 49


E-MTAB-5208:Plate 7 WT E9.5 5

text: The Francis Crick Institute

text: public

characteristic: SRA accession

text: E-MTAB-5208:Plate 7 WT E9.5 5

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: primary cell

text: epiblast cell

text: house mouse

characteristic: common name

text: embryonic day 9.5

text: wild type genotype

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: caudal lateral epiblast

text: Mus musculus

text: Plate 7 WT E9.5 5


E-MTAB-5208:Plate 7 WT E9.5 50

text: The Francis Crick Institute

text: public

text: E-MTAB-5208:Plate 7 WT E9.5 50

text: ArrayExpress

text: primary cell

text: epiblast cell

text: house mouse

characteristic: description

text: embryonic day 9.5

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

characteristic: growth condition

text: not applicable

text: Mus musculus

text: Mus musculus

text: caudal lateral epiblast

characteristic: organism part

text: Mus musculus

text: Plate 7 WT E9.5 50


E-MTAB-5208:Plate 7 WT E9.5 51

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: The Francis Crick Institute

text: public

text: E-MTAB-5208:Plate 7 WT E9.5 51

text: ArrayExpress

text: primary cell

text: epiblast cell

text: house mouse

text: embryonic day 9.5

text: wild type genotype

text: not applicable

text: Mus musculus

text: Mus musculus

text: caudal lateral epiblast

text: Mus musculus

characteristic: strain

text: Plate 7 WT E9.5 51


E-MTAB-5208:Plate 7 WT E9.5 52

text: The Francis Crick Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

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text: E-MTAB-5208:Plate 7 WT E9.5 52

text: ArrayExpress

text: primary cell

text: epiblast cell

text: house mouse

text: embryonic day 9.5

text: wild type genotype

text: not applicable

text: Mus musculus

text: Mus musculus

text: caudal lateral epiblast

text: Mus musculus

text: Plate 7 WT E9.5 52

characteristic: title


E-MTAB-5208:Plate 7 WT E9.5 53

text: The Francis Crick Institute

text: public

characteristic: SRA accession

text: E-MTAB-5208:Plate 7 WT E9.5 53

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: primary cell

text: epiblast cell

text: house mouse

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text: embryonic day 9.5

text: wild type genotype

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: caudal lateral epiblast

text: Mus musculus

text: Plate 7 WT E9.5 53


E-MTAB-5208:Plate 7 WT E9.5 54

text: The Francis Crick Institute

text: public

text: E-MTAB-5208:Plate 7 WT E9.5 54

text: ArrayExpress

text: primary cell

text: epiblast cell

text: house mouse

characteristic: description

text: embryonic day 9.5

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

characteristic: growth condition

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: caudal lateral epiblast

text: Mus musculus

text: Plate 7 WT E9.5 54


E-MTAB-5208:Plate 7 WT E9.5 55

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: The Francis Crick Institute

text: public

text: E-MTAB-5208:Plate 7 WT E9.5 55

text: ArrayExpress

text: primary cell

text: epiblast cell

text: house mouse

text: embryonic day 9.5

text: wild type genotype

text: not applicable

text: Mus musculus

text: Mus musculus

text: caudal lateral epiblast

characteristic: organism part

text: Mus musculus

text: Plate 7 WT E9.5 55


E-MTAB-5208:Plate 7 WT E9.5 56

text: The Francis Crick Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-5208:Plate 7 WT E9.5 56

text: ArrayExpress

text: primary cell

text: epiblast cell

text: house mouse

text: embryonic day 9.5

text: wild type genotype

text: not applicable

text: Mus musculus

text: Mus musculus

text: caudal lateral epiblast

text: Mus musculus

characteristic: strain

text: Plate 7 WT E9.5 56


E-MTAB-5208:Plate 7 WT E9.5 57

text: The Francis Crick Institute

text: public

characteristic: SRA accession

text: E-MTAB-5208:Plate 7 WT E9.5 57

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: primary cell

text: epiblast cell

text: house mouse

characteristic: common name

text: embryonic day 9.5

text: wild type genotype

text: not applicable

text: Mus musculus

text: Mus musculus

text: caudal lateral epiblast

text: Mus musculus

text: Plate 7 WT E9.5 57

characteristic: title


E-MTAB-5208:Plate 7 WT E9.5 58

text: The Francis Crick Institute

text: public

text: E-MTAB-5208:Plate 7 WT E9.5 58

text: ArrayExpress

text: primary cell

text: epiblast cell

text: house mouse

characteristic: description

text: embryonic day 9.5

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

characteristic: growth condition

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: caudal lateral epiblast

text: Mus musculus

text: Plate 7 WT E9.5 58


E-MTAB-5208:Plate 7 WT E9.5 59

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: The Francis Crick Institute

text: public

text: E-MTAB-5208:Plate 7 WT E9.5 59

text: ArrayExpress

text: primary cell

text: epiblast cell

text: house mouse

text: embryonic day 9.5

text: wild type genotype

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: caudal lateral epiblast

text: Mus musculus

text: Plate 7 WT E9.5 59


E-MTAB-5208:Plate 7 WT E9.5 6

text: The Francis Crick Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

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text: E-MTAB-5208:Plate 7 WT E9.5 6

text: ArrayExpress

text: primary cell

text: epiblast cell

text: house mouse

text: embryonic day 9.5

text: wild type genotype

text: not applicable

text: Mus musculus

text: Mus musculus

text: caudal lateral epiblast

characteristic: organism part

text: Mus musculus

text: Plate 7 WT E9.5 6


E-MTAB-5208:Plate 7 WT E9.5 60

text: The Francis Crick Institute

text: public

characteristic: SRA accession

text: E-MTAB-5208:Plate 7 WT E9.5 60

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: primary cell

text: epiblast cell

text: house mouse

characteristic: common name

text: embryonic day 9.5

text: wild type genotype

text: not applicable

text: Mus musculus

text: Mus musculus

text: caudal lateral epiblast

text: Mus musculus

characteristic: strain

text: Plate 7 WT E9.5 60


E-MTAB-5208:Plate 7 WT E9.5 61

text: The Francis Crick Institute

text: public

text: E-MTAB-5208:Plate 7 WT E9.5 61

text: ArrayExpress

text: primary cell

text: epiblast cell

text: house mouse

characteristic: description

text: embryonic day 9.5

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

characteristic: growth condition

text: not applicable

text: Mus musculus

text: Mus musculus

text: caudal lateral epiblast

text: Mus musculus

text: Plate 7 WT E9.5 61

characteristic: title


E-MTAB-5208:Plate 7 WT E9.5 62

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: The Francis Crick Institute

text: public

text: E-MTAB-5208:Plate 7 WT E9.5 62

text: ArrayExpress

text: primary cell

text: epiblast cell

text: house mouse

text: embryonic day 9.5

text: wild type genotype

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: caudal lateral epiblast

text: Mus musculus

text: Plate 7 WT E9.5 62


E-MTAB-5208:Plate 7 WT E9.5 63

text: The Francis Crick Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-5208:Plate 7 WT E9.5 63

text: ArrayExpress

text: primary cell

text: epiblast cell

text: house mouse

text: embryonic day 9.5

text: wild type genotype

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: caudal lateral epiblast

text: Mus musculus

text: Plate 7 WT E9.5 63


E-MTAB-5208:Plate 7 WT E9.5 64

text: The Francis Crick Institute

text: public

characteristic: SRA accession

text: E-MTAB-5208:Plate 7 WT E9.5 64

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: primary cell

text: epiblast cell

text: house mouse

characteristic: common name

text: embryonic day 9.5

text: wild type genotype

text: not applicable

text: Mus musculus

text: Mus musculus

text: caudal lateral epiblast

characteristic: organism part

text: Mus musculus

text: Plate 7 WT E9.5 64


E-MTAB-5208:Plate 7 WT E9.5 65

text: The Francis Crick Institute

text: public

text: E-MTAB-5208:Plate 7 WT E9.5 65

text: ArrayExpress

text: primary cell

text: epiblast cell

text: house mouse

characteristic: description

text: embryonic day 9.5

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

characteristic: growth condition

text: not applicable

text: Mus musculus

text: Mus musculus

text: caudal lateral epiblast

text: Mus musculus

characteristic: strain

text: Plate 7 WT E9.5 65


E-MTAB-5208:Plate 7 WT E9.5 66

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: The Francis Crick Institute

text: public

text: E-MTAB-5208:Plate 7 WT E9.5 66

text: ArrayExpress

text: primary cell

text: epiblast cell

text: house mouse

text: embryonic day 9.5

text: wild type genotype

text: not applicable

text: Mus musculus

text: Mus musculus

text: caudal lateral epiblast

text: Mus musculus

text: Plate 7 WT E9.5 66

characteristic: title


E-MTAB-5208:Plate 7 WT E9.5 7

text: The Francis Crick Institute

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: E-MTAB-5208:Plate 7 WT E9.5 7

text: ArrayExpress

text: primary cell

text: epiblast cell

text: house mouse

text: embryonic day 9.5

text: wild type genotype

text: not applicable

characteristic: isolate

text: Mus musculus

text: Mus musculus

text: caudal lateral epiblast

text: Mus musculus

text: Plate 7 WT E9.5 7


E-MTAB-5208:Plate 7 WT E9.5 8

text: The Francis Crick Institute

text: public

characteristic: SRA accession

text: E-MTAB-5208:Plate 7 WT E9.5 8

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: primary cell

text: epiblast cell

text: house mouse

characteristic: common name

text: embryonic day 9.5

text: wild type genotype

text: not applicable

text: Mus musculus

characteristic: organism

text: Mus musculus

text: caudal lateral epiblast

text: Mus musculus

text: Plate 7 WT E9.5 8


E-MTAB-5208:Plate 7 WT E9.5 9

text: The Francis Crick Institute

text: public

text: E-MTAB-5208:Plate 7 WT E9.5 9

text: ArrayExpress

text: primary cell

text: epiblast cell

text: house mouse

characteristic: description

text: embryonic day 9.5

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

characteristic: growth condition

text: not applicable

text: Mus musculus

text: Mus musculus

text: caudal lateral epiblast

characteristic: organism part

text: Mus musculus

text: Plate 7 WT E9.5 9


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Saolta

text: public

text: IRL

characteristic: collection date

text: Klebsiella

characteristic: genus

text: Ireland

characteristic: geographic location (country and/or sea)

text: Homo sapiens

characteristic: host

text: not applicable

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: Human

text: gastrointestinal system

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae

type: AMR curated data - CABBAGE

value: ertapenem

value: EUCAST

value: broth dilution

value: Vitek

value: resistant

value: Klebsiella pneumoniae

value: tigecycline

value: susceptible

value: amikacin

value: ampicillin

value: gentamicin

value: tobramycin

value: cefoxitin

value: amoxicillin-clavulanic acid

value: cefpodoxime

value: cefotaxime

value: piperacillin-tazobactam

value: meropenem

value: intermediate


text: Saolta

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: IRL

characteristic: SRA accession

characteristic: Submitter Id

text: Klebsiella

text: Ireland

text: Homo sapiens

text: not applicable

text: Homo sapiens

characteristic: isolate

text: Human

text: gastrointestinal system

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae

characteristic: title

value: EUCAST

value: broth dilution

value: cefpodoxime-clavulanic acid

value: ertapenem


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Saolta

text: public

text: IRL

text: Klebsiella

text: Ireland

text: Homo sapiens

text: not applicable

text: Homo sapiens

text: Human

text: gastrointestinal system

text: Klebsiella pneumoniae

characteristic: organism

text: Klebsiella pneumoniae

value: Vitek

value: resistant

value: Klebsiella pneumoniae

value: tigecycline


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Saolta

text: public

text: IRL

characteristic: collection date

text: Klebsiella

text: Ireland

text: Homo sapiens

text: not applicable

text: Homo sapiens

text: Human

text: gastrointestinal system

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae

value: gentamicin

value: susceptible

value: amikacin


text: Saolta

text: public

text: IRL

text: Klebsiella

characteristic: genus

text: Ireland

characteristic: geographic location (country and/or sea)

text: Homo sapiens

characteristic: host

text: not applicable

text: Homo sapiens

text: Human

text: urinary tract

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae

value: ampicillin

value: cefoxitin

value: tobramycin

value: amoxicillin-clavulanic acid


text: Saolta

characteristic: INSDC center name

text: public

text: IRL

text: Klebsiella

text: Ireland

text: Homo sapiens

text: not applicable

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: Human

text: gastrointestinal system

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae

value: meropenem

value: piperacillin-tazobactam

value: cefotaxime

value: cefpodoxime


text: Saolta

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: IRL

text: Klebsiella

text: Ireland

text: Homo sapiens

text: not applicable

text: Homo sapiens

text: Human

text: gastrointestinal system

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae

value: EUCAST

value: broth dilution

value: cefpodoxime-clavulanic acid

value: intermediate


text: Saolta

text: public

text: IRL

characteristic: SRA accession

characteristic: Submitter Id

text: Klebsiella

text: Ireland

text: Homo sapiens

text: not applicable

text: Homo sapiens

characteristic: isolate

text: Human

text: gastrointestinal system

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae

value: Vitek

value: resistant

value: Klebsiella pneumoniae

value: ertapenem


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Saolta

text: public

text: IRL

text: Klebsiella

text: Ireland

text: Homo sapiens

text: not applicable

text: Homo sapiens

text: Human

text: urinary tract

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae

characteristic: title

value: susceptible

value: gentamicin

value: amikacin


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: URMITE

text: public

text: Culturomics to increase the human bacterial repertoire

characteristic: description

text: Bacillus sonorensis

characteristic: organism

text: Bacillus sonorensis

characteristic: strain

text: Bacillus sonorensis genome


text: URMITE

characteristic: INSDC center name

text: public

text: Marinococcus halophilus

text: Marinococcus halophilus

text: Marinococcus halophilus genome


text: URMITE

text: public

text: Halobacillus massiliensis strain Marseille-P3554T was isolated from stool sample

text: Halobacillus massiliensis

text: Halobacillus massiliensis

text: Halobacillus massiliensis genome


text: CNRS UMR 5088

text: public

tag: Namespace:CNRS UMR 5088

text: ArrayExpress

characteristic: broker name

text: human

characteristic: common name

text: Protocols: DIvA (AsiSI-ER-U20S)cells were cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with antibiotics, 10% FCS (Invitrogen) and 1 μg/mL puromycin (DIvA cells) at 37 °C under a humidified atmosphere with 5% CO2. For cell synchronization in G1, cells were incubated with 2 mM thymidine for 16 h, released for 14 h and subjected to the second thymidine treatment for 19 h. G1 cells (12h after release) were treated with 4OHT (4h) followed by an auxin treatment of 2h when indicated. For AsiSI-dependent DSB induction, cells were treated with 300 nM 4OHT (Sigma, H7904) for 4 h. After 4OHT and or auxin treatment in G1 synchronized cells, DSBs labelling was carried out using BLESS method as described previously (Crosetto et al, 2013). Briefly, cells were fixed with formaldehyde, lysed and mildly digested with proteinase K at 37ºC in order to purify intact nuclei, then DSBs were blunted and 5'phosphorylated using Quick Blunting Kit (NEB). Subsequently, a biotinylated proximal linker (Sigma) was ligated to DSBs using T4 ligase (NEB). Next, DNA was extracted by precipitation with isopropanol and sonicated using Covaris S220 ultrasonicator to create fragments approximately 400bp long. Labeled, biotinylated fragments were captured on streptavidin beads (Invitrogen) and ligated to a distal linker (Sigma). Resulting DNA fragments were then linearized by I-SceI (NEB) digestion and amplified by PCR. Libraries were prepared using TruSeq DNA LT Sample Prep Kit (Illumina), followed by 2x60 bp next generation sequencing on Illumina HiSeq 2500.

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens


text: CNRS UMR 5088

characteristic: INSDC first public

text: public

text: E-MTAB-48461487005187:BLESS_G1_undamaged

text: ArrayExpress

text: human

text: not applicable

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens


text: University Hospital Galway

characteristic: INSDC last update

text: public

tag: Namespace:University Hospital Galway

characteristic: collection date

text: Ireland

characteristic: geographic location (country and/or sea)

text: not applicable

characteristic: host health state

text: Homo sapiens

characteristic: host scientific name

text: Human

text: Salmonella enterica subsp. enterica serovar Panama

text: Panama

characteristic: serovar

text: Subsp. I


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: University Hospital Galway

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Ireland

text: not applicable

text: Homo sapiens

characteristic: isolate

text: Human

text: Salmonella enterica subsp. enterica serovar Kintambo

text: Kintambo

text: Subsp. I


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University Hospital Galway

text: public

text: Ireland

text: not applicable

text: Homo sapiens

text: Human

text: Salmonella enterica subsp. enterica serovar Typhimurium

characteristic: organism

text: Typhimurium

text: Subsp. I

characteristic: title


text: University Hospital Galway

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Ireland

text: not applicable

text: Homo sapiens

text: Human

text: Salmonella enterica subsp. enterica serovar Isangi

text: Isangi

text: Subsp. I


text: University Hospital Galway

characteristic: INSDC last update

text: public

characteristic: collection date

text: Ireland

text: not applicable

text: Homo sapiens

text: Human

text: Salmonella enterica subsp. enterica serovar Typhimurium var. monophasic 4,[5],12:i:-

text: Subsp. I


text: University Hospital Galway

text: public

text: Ireland

characteristic: geographic location (country and/or sea)

text: not applicable

characteristic: host health state

text: Homo sapiens

text: Human

text: Salmonella enterica subsp. enterica serovar Newport

text: Newport

text: Subsp. I


text: University Hospital Galway

text: public

text: Ireland

text: not applicable

text: Homo sapiens

characteristic: host scientific name

text: Human

text: Salmonella enterica subsp. enterica serovar Java

text: Java

characteristic: serovar

text: Subsp. I


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: IRD

text: public

text: thale cress

characteristic: common name

text: Mobilome-seq on Epigenetics Recombinant inbred (Epi12) siliques samples

characteristic: description

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: Mobilome-seq epi12 library


text: IRD

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Japanese rice

text: Oryza sativa Japonica Group

text: Oryza sativa Japonica Group

text: Mobilome-seq Oryza sativa callus library


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: IRD

text: public

text: Japanese rice

text: Oryza sativa Japonica Group

characteristic: organism

text: Oryza sativa Japonica Group

text: Mobilome-seq Oryza sativa leaf library


text: IRD

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Japanese rice

text: Oryza sativa Japonica Group

text: Oryza sativa Japonica Group

text: Mobilome-seq Oryza sativa seed library

characteristic: title


text: IRD

characteristic: INSDC last update

text: public

text: thale cress

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: Mobilome-seq Col WT library


text: public

text: E-MTAB-45681487005959:Rpb2-FLAG_glu_repeat1

tag: Namespace:Department of Biochemistry, University of Oxford

text: ArrayExpress

characteristic: broker name

text: baker's yeast

text: Protocols: Cells were grown in 25 ml YPD (1 % yeast extract (Difco), 1 % bactopeptone, 2 % glucose) overnight. This was used to inoculate 1 L of YPD in a 2 L flask to give a starting OD600 of 0.2. 2 L of cells were grown (30oC, 170 rpm) to OD600 of 0.65 (mid-log) and harvested by filtration onto a 0.45 µm pore size, 90 mm diameter nitrocellulose filter paper (Whatman). Cells were scraped off the filter paper with a spatula pre-cooled in liquid nitrogen and flash frozen in liquid nitrogen. Cells were ground in 6 cycles of 3 min at a 15 Hz shaking frequency in a 50 ml grinding jar (Retch) with a 25 mm stainless steel ball using a Retsch MM400 mixer mill. The grinding chamber was cooled in liquid nitrogen in between cycles. 1 g of yeast grindate was stored at -80 oC. For carbon source shift experiments 2 L of cells were grown to mid-log in 2 x 2 L flasks, then centrifuged (3000 rpm, 3 min) and then resuspended in 2 L YPG (1 % yeast extract (Difco), 1 % bactopeptone, 2 % galactose) prewarmed to 30oC. Resuspended cells were returned to 2 L flasks, incubated (30oC, 170 rpm) and harvested by filtration as above after the requisite incubation time. 1 g of yeast grindate was resuspended in Lysis Buffer A (20 mM HEPES (pH 7.4), 110 mM KOAc, 0.5 % Triton-X-100, 0.1 % Tween 20, 10 mM MnCl2, 1x proteinase inhibitors (complete, EDTA-free (Roche)), 50 U/ml SUPERase.In (Invitrogen)). Resuspended grindate was incubated (4 oC, 20 min) with 660 U of DNase I (Promega). Insoluble cell debris was pelleted by spinning (16,000 g, 4 oC, 10 min). Supernatants were combined and added to 0.5 ml of anti-FLAG M2 affinity agarose beads (Sigma) pre-washed with 2 x 10 ml Lysis buffer A (without SUPERase.In). Beads and supernatant were incubated (4 oC, 2.5 h) on a nutator, before spinning (1,000 g, 4 oC, 2 min). Beads were washed four times with 10 ml Wash Buffer A (20 mM HEPES (pH 7.4), 110 mM KOAc, 0.5 % Triton-X-100, 0.1 % Tween 20, 1 mM EDTA). Beads were incubated (30 min, 4 oC) twice with 300 µl of Elution Buffer (Lysis Buffer A with 1 mg/ml 3 x FLAG peptide (Sigma)). The elution supernatants were combined and RNAs were extracted using a miRNeasy kit (Qiagen) according to the manufacturer’s instructions. For the sequential IP procedure, the elution supernatants from two IPs were combined giving a total of 1200 ul and added to 15 ul anti-HA dynabeads (Pierce) pre-washed with 2 x 1 ml Lysis buffer A. Beads and supernatant were incubated (4 oC, 2.5 h) on a nutator. Beads were washed five times with 1 ml Wash Buffer A. Beads were resuspended in 100 ul Lysis buffer A and RNAs were extracted using a miRNeasy kit (Qiagen) according to the manufacturer’s instructions. Library generation steps: Linker ligation and RNA fragmentation. Immunoprecipitated RNA (3 μg in 30 µl 10 mM Tris-HCl, pH 7.0) was denatured (2 min, 80 oC) and placed on ice. A 5’ adenylated, 3’ blocked with a dideoxy C base cloning linker 5rApp/CTGTAGGCACCATCAAT/3ddC (Integrated DNA Technologies) was ligated to the 3’ ends of RNAs by first dividing the RNA into three microfuge tubes and then adding 10 µl ligation reaction mix to give final concentrations of 50 ng µl-1 cloning linker 1, 12 % PEG 8000, 1 x T4 RNA ligase 2 (Rnl2) (truncated) ligation buffer, 10 U µl-1 T4 Rnl2 (truncated) (NEB) and incubating for 3 h at 37 oC. Ligated RNA was incubated (95 oC, 35 min) with 20 µl Alkaline Fragmentation Buffer (100 mM NaCO3 (pH 9.2), 2 mM EDTA) to fragment linker ligated RNA to a narrow size range to reduce size bias of future steps. RNA was precipitated by incubating (30 min, -20 oC) with ice cold 500 µl H20, 60 µl 3 M NaOAc (pH 5.5), 2 µl 15 mg ml-1 GlycoBlue (Ambion) and 0.75 ml isopropanol and then spinning (16,000 g, 4 oC, 30 min). Pellets were washed with 0.75 ml 80 % ethanol, dried (10 min, room temperature) and resuspended sequentially in the same 10 µl 10 mM Tris-HCl (pH 7.0). Size selection. Ligated and fragmented RNA was mixed with 10 µl 2 x TBE-urea loading dye (89 mM Tris-HCl, 89 mM Boric acid, 2 mM EDTA, 12 % Ficoll, 7 M Urea, 2.5 mg/ml Orange G), denatured (2 min, 80 oC) and run on a 10 well 10 % TBE-urea gel (Biorad) (200 V, 32 min). The gel was stained with SYBRGold (Invitrogen) and the region containing 38-95nt fragments excised. This gel piece was divided between three microfuge tubes and physically disrupted. Each was incubated (70oC, 10 min) in 200 µl H20. The tubes were pooled and gel debris was removed using a Costar-Spin-X column (Corning). RNA was precipitated by adding 60 µl 3 M NaOAc (pH 5.5), 2 µl 15 mg ml-1 GlycoBlue and 0.75 ml isopropanol, incubating (30 min, -20oC) and then spinning (16,000 g, 4oC, 30 min). Pellets were washed with 0.75 ml 80 % ethanol, dried (10 min, room temperature) and resuspended in 10 µl 10 mM Tris-HCl (pH 7.0). Reverse transcription and circularisation. Reverse transcription (RT) was carried out by adding 3.28 µl 5 x FS buffer, 0.82 µl dNTPs (10 mM each), 0.5 µl 100 µM RT primer (5’/5Phos/ATCTCGTATGCCGTCTTCTGCTTG/iSp18/CACTCA/iSp18/TCCGACGATCATTGATGGTGCCTACAG 3’; This is phosphorylated at the 5’ end (5 Phos) for later circularization and contains two 18 carbon spacer sequences (iSp18)), denaturing (80 oC, 2 min) and incubating (48oC, 30 min) with 0.5 µl Superase.In, 0.82 µl 0.1 M DTT, 0.82 µl Superscript III (Invitrogen). 1.8 µl 1 M NaOH was added and RNA was degraded (98 oC, 20 min). To neutralize, 1.8 µl 1 M HCl was added. cDNA was mixed with 20 µl 2 x TBE-urea loading dye, denatured (3 min, 95 oC), and run (loaded in 2 wells, 20 µl per well) on a 10 well 10 % TBE-urea gel (Biorad) (200 V, 50 min). The gel was stained with SYBRGold (Invitrogen) and the regions containing the RT product excised. Both gel pieces were physically disrupted and incubated (70 oC, 10 min) in 200 µl H20 in separate tubes. Gel debris was removed and tubes were pooled. cDNA was precipitated by adding 25 µl 3 M NaCl, 2 µl GlycoBlue and 0.75 ml isopropanol, incubating (-20 oC, 30 min) and then spinning (16,000 g, 4 oC, 30 min). The pellet was washed with 0.75 ml 80 % ethanol, dried (10 min, room temperature) and resuspended in 15 µl 10 mM Tris-HCl (pH 8.0). The RT product was circularized by incubating (60 oC, 60 min) with 2 µl 10 x CircLigase buffer, 1 µl 1 mM ATP, 1 µl 50 mM MnCl2, 1 µl CircLigase (Epicentre). The enzyme was heat-inactivated at 80 oC, 10 min. PCR amplification. 16.7 µl 5 x HF Phusion buffer, 1.7 µl dNTPs (10 mM), 0.4 µl 100 µM Barcoded primer (A, AATGATACGGCGACCACCGAGATCTACACGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGCCATCCGACGATCATTGATGG ;B, AATGATACGGCGACCACCGAGATCTACACGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCATCTCCGACGATCATTGATGG; C, AATGATACGGCGACCACCGAGATCTACACGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCTCCGACGATCATTGATGG; or D, AATGATACGGCGACCACCGAGATCTACACGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCATCCGACGATCATTGATGG;), 0.4 µl 100 µM Primer 1 (CAAGCAGAAGACGGCATACGA), 59.2 µl H20, 0.8 µl Phusion polymerase (NEB) was added to 5 µl circularized DNA. This was divided (16.7 µl per tube) between five 0.2 ml tubes. PCR reactions were heated (98oC, 30 s) and then submitted to 7 temperature cycles (98oC, 10 s; 60oC, 10 s; 72oC, 10 s). At the end of cycle 3 and each subsequent cycle one tube was removed and placed on ice. 3.4 µl DNA loading dye (1.5 g Ficoll 400, 25 mg Orange G in 10 ml H20) was added to each reaction and run on an 8 % TBE gel (Invitrogen) (180 V, 55 min). The gel was stained with SYBRGold and the PCR product excised from the PCR reaction with the highest unsaturated signal without higher molecular weight products. The gel piece was physically disrupted and incubated (room temperature, overnight, with agitation) in 0.67 ml DNA soaking buffer (0.3 M NaCl, 10 mM Tris-HCl pH 8.0, 1 mM EDTA). DNA was precipitated by adding 2 µl GlycoBlue and 0.68 ml isopropanol, incubating (-20oC, 30 min) and spinning (16,000 g, 4oC, 30 min). The pellet was washed with 0.75 ml 80 % ethanol, dried (10 min, room temperature) and resuspended in 10 µl 10 mM Tris-HCl (pH 8.0).

characteristic: genotype

text: Glucose (YPD)

characteristic: growth condition

text: not applicable

characteristic: isolate

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Department of Biochemistry, University of Oxford

text: public

text: E-MTAB-45681487005959:Sequential_IP_No-HA-tag_Rpb3-FLAG_glu_repeat1

text: ArrayExpress

text: baker's yeast

characteristic: common name

text: Rpb3-3xFLAG, Non-HA-tagged

text: Glucose (YPD)

text: not applicable

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-45681487005959:Sequential_IP_Paf1-HA_glu_repeat1

text: ArrayExpress

text: baker's yeast

characteristic: description

text: Rpb3-3xFLAG, Paf1-3xHA

text: Glucose (YPD)

text: not applicable

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-45681487005959:Sequential_IP_Rpb2-HA_Rpb3-FLAG-tag_glu_repeat1

characteristic: Submitter Id

text: ArrayExpress

text: baker's yeast

text: Rpb3-3xFLAG, Rpb2-3xHA

text: Glucose (YPD)

text: not applicable

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae


E-MTAB-5473:A17 Mock  7 dpi Rep1

text: CSIRO Agriculture and Food

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-5473:A17 Mock 7 dpi Rep1

tag: Namespace:CSIRO Agriculture and Food

text: ArrayExpress

text: barrel medic

text: Protocols: M. truncatula lines A17 and skl were treated, grown and inoculated according to (Anderson et al., 2010). R. solani AG8 (isolate WAC10335) was prepared according to (Lichtenzveig et al., 2006). Briefly, surface sterilised M. truncatula seeds were germinated on moist filter paper in the dark at 4 °C for two days, then one day at 24 °C. Seedlings were added to pots half filled with vermiculite also containing 5 ml of 2.2mg/ml dry weight equivalent suspension of homogenised fungal mycelium. The seedlings were covered with fine vermiculite and transferred to growth rooms at a constant temperature of 24 °C with 16/8 light/dark photoperiod at a light intensity of 200 mmol m-2 s-1. Mock treated plants were treated the same with the addition of 5 ml sterile water rather than the 5 ml of R. solani suspension. Inoculated plants were harvested at two and seven days after planting into pre-inoculated pots with corresponding mock samples taken at the same time. Root and above ground tissue was collected separately, immediately frozen in liquid nitrogen and stored at -80 °C until RNA extraction. The remaining plants were scored for survival and harvested at 21 days after inoculation to confirm the infection had progressed as previously described (Anderson et al., 2013). Frozen root tissue was ground in the presence of liquid nitrogen and total RNA extracted using Trizol reagent (Sigma) according to manufacturer’s instructions. Libraries were prepared using the illumina TruSeq Stranded mRNA Library Prep Kit according to manufacturer’s instructions.

characteristic: ecotype

text: not applicable

text: Medicago truncatula

characteristic: organism

text: Medicago truncatula

text: root

characteristic: organism part

text: Medicago truncatula

text: A17 Mock 7 dpi Rep1


E-MTAB-5473:A17 Mock  7 dpi Rep2

text: CSIRO Agriculture and Food

characteristic: INSDC last update

text: public

text: E-MTAB-5473:A17 Mock 7 dpi Rep2

text: ArrayExpress

characteristic: broker name

text: barrel medic

text: not applicable

text: Medicago truncatula

text: Medicago truncatula

text: root

text: Medicago truncatula

text: A17 Mock 7 dpi Rep2

characteristic: title


E-MTAB-5473:A17 Mock  7 dpi Rep3

characteristic: ENA-CHECKLIST

text: CSIRO Agriculture and Food

text: public

text: E-MTAB-5473:A17 Mock 7 dpi Rep3

text: ArrayExpress

text: barrel medic

text: not applicable

characteristic: isolate

text: Medicago truncatula

text: Medicago truncatula

text: root

text: Medicago truncatula

text: A17 Mock 7 dpi Rep3


E-MTAB-5473:A17 Mock 2 dpi Rep1

characteristic: ENA-FIRST-PUBLIC

text: CSIRO Agriculture and Food

text: public

text: E-MTAB-5473:A17 Mock 2 dpi Rep1

text: ArrayExpress

text: barrel medic

characteristic: common name

text: not applicable

text: Medicago truncatula

text: Medicago truncatula

text: root

text: Medicago truncatula

text: A17 Mock 2 dpi Rep1


E-MTAB-5473:A17 Mock 2 dpi Rep2

text: CSIRO Agriculture and Food

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-5473:A17 Mock 2 dpi Rep2

text: ArrayExpress

text: barrel medic

text: not applicable

text: Medicago truncatula

text: Medicago truncatula

text: root

text: Medicago truncatula

text: A17 Mock 2 dpi Rep2


E-MTAB-5473:A17 Mock 2 dpi Rep3

characteristic: ENA-LAST-UPDATE

text: CSIRO Agriculture and Food

text: public

text: E-MTAB-5473:A17 Mock 2 dpi Rep3

text: ArrayExpress

text: barrel medic

characteristic: description

text: not applicable

text: Medicago truncatula

text: Medicago truncatula

text: root

text: Medicago truncatula

text: A17 Mock 2 dpi Rep3


name: E-MTAB-5473:A17 R. solani inoculated  2 dpi Rep1

characteristic: External Id

text: CSIRO Agriculture and Food

text: public

characteristic: Submitter Id

text: ArrayExpress

text: barrel medic

text: not applicable

text: Medicago truncatula

text: Medicago truncatula

text: root

text: Medicago truncatula

text: A17 R. solani inoculated 2 dpi Rep1


name: E-MTAB-5473:A17 R. solani inoculated  2 dpi Rep2

text: CSIRO Agriculture and Food

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ArrayExpress

text: barrel medic

text: not applicable

text: Medicago truncatula

text: Medicago truncatula

text: root

text: Medicago truncatula

text: A17 R. solani inoculated 2 dpi Rep2


name: E-MTAB-5473:A17 R. solani inoculated  2 dpi Rep3

text: CSIRO Agriculture and Food

text: public

text: ArrayExpress

text: barrel medic

characteristic: ecotype

text: not applicable

text: Medicago truncatula

characteristic: organism

text: Medicago truncatula

text: root

text: Medicago truncatula

text: A17 R. solani inoculated 2 dpi Rep3


name: E-MTAB-5473:A17 R. solani inoculated  7 dpi Rep1

text: CSIRO Agriculture and Food

characteristic: INSDC last update

text: public

text: ArrayExpress

text: barrel medic

text: not applicable

text: Medicago truncatula

text: Medicago truncatula

text: root

characteristic: organism part

text: Medicago truncatula

text: A17 R. solani inoculated 7 dpi Rep1


name: E-MTAB-5473:A17 R. solani inoculated  7 dpi Rep2

text: CSIRO Agriculture and Food

text: public

text: ArrayExpress

characteristic: broker name

text: barrel medic

text: not applicable

text: Medicago truncatula

text: Medicago truncatula

text: root

text: Medicago truncatula

text: A17 R. solani inoculated 7 dpi Rep2

characteristic: title


name: E-MTAB-5473:A17 R. solani inoculated  7 dpi Rep3

characteristic: ENA-CHECKLIST

text: CSIRO Agriculture and Food

text: public

text: ArrayExpress

text: barrel medic

text: not applicable

characteristic: isolate

text: Medicago truncatula

text: Medicago truncatula

text: root

text: Medicago truncatula

text: A17 R. solani inoculated 7 dpi Rep3


E-MTAB-5473:skl Mock 2 dpi Rep1

characteristic: ENA-FIRST-PUBLIC

text: CSIRO Agriculture and Food

text: public

text: E-MTAB-5473:skl Mock 2 dpi Rep1

text: ArrayExpress

text: barrel medic

characteristic: common name

text: sickle mutant

text: not applicable

text: Medicago truncatula

text: Medicago truncatula

text: root

text: Medicago truncatula

text: skl Mock 2 dpi Rep1


E-MTAB-5473:skl Mock 2 dpi Rep2

text: CSIRO Agriculture and Food

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-5473:skl Mock 2 dpi Rep2

text: ArrayExpress

text: barrel medic

text: sickle mutant

text: not applicable

text: Medicago truncatula

text: Medicago truncatula

text: root

text: Medicago truncatula

text: skl Mock 2 dpi Rep2


E-MTAB-5473:skl Mock 2 dpi Rep3

characteristic: ENA-LAST-UPDATE

text: CSIRO Agriculture and Food

text: public

text: E-MTAB-5473:skl Mock 2 dpi Rep3

text: ArrayExpress

text: barrel medic

characteristic: description

text: sickle mutant

text: not applicable

text: Medicago truncatula

text: Medicago truncatula

text: root

text: Medicago truncatula

text: skl Mock 2 dpi Rep3


E-MTAB-5473:skl Mock 7 dpi Rep1

characteristic: External Id

text: CSIRO Agriculture and Food

text: public

text: E-MTAB-5473:skl Mock 7 dpi Rep1

characteristic: Submitter Id

text: ArrayExpress

text: barrel medic

text: sickle mutant

text: not applicable

text: Medicago truncatula

text: Medicago truncatula

text: root

text: Medicago truncatula

text: skl Mock 7 dpi Rep1


E-MTAB-5473:skl Mock 7 dpi Rep2

text: CSIRO Agriculture and Food

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: E-MTAB-5473:skl Mock 7 dpi Rep2

text: ArrayExpress

text: barrel medic

text: sickle mutant

text: not applicable

text: Medicago truncatula

text: Medicago truncatula

text: root

text: Medicago truncatula

text: skl Mock 7 dpi Rep2


E-MTAB-5473:skl Mock 7 dpi Rep3

text: CSIRO Agriculture and Food

text: public

text: E-MTAB-5473:skl Mock 7 dpi Rep3

text: ArrayExpress

text: barrel medic

text: sickle mutant

characteristic: ecotype

text: not applicable

text: Medicago truncatula

characteristic: organism

text: Medicago truncatula

text: root

text: Medicago truncatula

text: skl Mock 7 dpi Rep3


name: E-MTAB-5473:skl R. solani inoculated 2 dpi Rep1

text: CSIRO Agriculture and Food

characteristic: INSDC last update

text: public

text: ArrayExpress

text: barrel medic

text: sickle mutant

text: not applicable

text: Medicago truncatula

text: Medicago truncatula

text: root

characteristic: organism part

text: Medicago truncatula

text: skl R. solani inoculated 2 dpi Rep1


name: E-MTAB-5473:skl R. solani inoculated 2 dpi Rep2

text: CSIRO Agriculture and Food

text: public

text: ArrayExpress

characteristic: broker name

text: barrel medic

text: sickle mutant

text: not applicable

text: Medicago truncatula

text: Medicago truncatula

text: root

text: Medicago truncatula

text: skl R. solani inoculated 2 dpi Rep2

characteristic: title


name: E-MTAB-5473:skl R. solani inoculated 2 dpi Rep3

characteristic: ENA-CHECKLIST

text: CSIRO Agriculture and Food

text: public

text: ArrayExpress

text: barrel medic

text: sickle mutant

text: not applicable

characteristic: isolate

text: Medicago truncatula

text: Medicago truncatula

text: root

text: Medicago truncatula

text: skl R. solani inoculated 2 dpi Rep3


name: E-MTAB-5473:skl R. solani inoculated 7 dpi Rep1

characteristic: ENA-FIRST-PUBLIC

text: CSIRO Agriculture and Food

text: public

text: ArrayExpress

text: barrel medic

characteristic: common name

text: sickle mutant

text: not applicable

text: Medicago truncatula

text: Medicago truncatula

text: root

text: Medicago truncatula

text: skl R. solani inoculated 7 dpi Rep1


name: E-MTAB-5473:skl R. solani inoculated 7 dpi Rep2

text: CSIRO Agriculture and Food

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

text: barrel medic

text: sickle mutant

text: not applicable

text: Medicago truncatula

text: Medicago truncatula

text: root

text: Medicago truncatula

text: skl R. solani inoculated 7 dpi Rep2


name: E-MTAB-5473:skl R. solani inoculated 7 dpi Rep3

characteristic: ENA-LAST-UPDATE

text: CSIRO Agriculture and Food

text: public

text: ArrayExpress

text: barrel medic

characteristic: description

text: sickle mutant

text: not applicable

text: Medicago truncatula

text: Medicago truncatula

text: root

text: Medicago truncatula

text: skl R. solani inoculated 7 dpi Rep3


characteristic: External Id

text: UCSDMI

text: public

text: qiita_sid_10815:10815.1A066AnpW33LAM2012

characteristic: Submitter Id

text: UBERON:multicellular organism

text: UBERON:tissue

text: UBERON:gut

text: Anopheles punctipennis 1A066AnpW33LAM2012

text: TRUE

characteristic: elevation

text: temperate biome

text: insecta-associated habitat

text: organic material

text: host-associated

text: Canada

text: LAM

text: Anopheles punctipennis

characteristic: host scientific name

text: mosquito

characteristic: latitude

characteristic: longitude

text: mosquito metagenome

text: U Toronto

text: FALSE

text: TRUE

characteristic: public

text: mosquito tissue

text: mosquito metagenome

text: Anp

text: Anopheles punctipennis

characteristic: wnv


text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: UBERON:multicellular organism

text: UBERON:tissue

text: UBERON:gut

text: Coquilletidia perturbans 1A066CqW33LAM2012

text: TRUE

text: temperate biome

text: insecta-associated habitat

text: organic material

text: host-associated

text: Canada

text: LAM

text: Coquillettidia perturbans

text: mosquito

text: mosquito metagenome

characteristic: organism

text: U Toronto

text: FALSE

text: TRUE

text: mosquito tissue

text: mosquito metagenome

text: Cq

text: Coquilletidia perturbans

characteristic: title

text: unknown


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UCSDMI

text: public

characteristic: INSDC status

text: UBERON:multicellular organism

text: UBERON:tissue

text: UBERON:gut

text: Aedes vexans complex 1F003AvW32LAM2012

text: TRUE

text: temperate biome

text: insecta-associated habitat

text: organic material

text: host-associated

text: Canada

text: LAM

text: Aedes vexans complex

text: mosquito

text: mosquito metagenome

text: U Toronto

text: FALSE

text: TRUE

text: mosquito tissue

text: mosquito metagenome

text: Av

text: Aedes vexans complex

text: unknown


characteristic: ENA-LAST-UPDATE

text: UCSDMI

text: public

characteristic: SRA accession

text: UBERON:multicellular organism

text: UBERON:tissue

text: UBERON:gut

text: Coquilletidia perturbans 1F003CqW32LAM2012

characteristic: description

text: TRUE

text: temperate biome

text: insecta-associated habitat

text: organic material

text: host-associated

text: Canada

text: LAM

text: Coquillettidia perturbans

text: mosquito

text: mosquito metagenome

text: U Toronto

text: FALSE

text: TRUE

text: mosquito tissue

text: mosquito metagenome

text: Cq

text: Coquilletidia perturbans

text: unknown


characteristic: External Id

text: UCSDMI

text: public

characteristic: Submitter Id

text: UBERON:multicellular organism

text: UBERON:tissue

text: UBERON:gut

text: Culex pipiens/restuans 1F003CxW33LAM2012

text: TRUE

characteristic: elevation

text: temperate biome

text: insecta-associated habitat

text: organic material

text: host-associated

text: Canada

text: LAM

text: Culex pipiens/restuans

text: mosquito

text: mosquito metagenome

text: U Toronto

text: FALSE

text: TRUE

text: mosquito tissue

text: mosquito metagenome

text: Cx

text: Culex pipiens/restuans


text: UCSDMI

text: public

text: UBERON:multicellular organism

text: UBERON:tissue

text: UBERON:gut

text: Culex pipiens/restuans 1F003CxW34LAM2012

text: TRUE

text: temperate biome

text: insecta-associated habitat

text: organic material

text: host-associated

text: Canada

text: LAM

text: Culex pipiens/restuans

characteristic: host scientific name

text: mosquito

characteristic: latitude

characteristic: longitude

text: mosquito metagenome

text: U Toronto

text: FALSE

text: TRUE

text: mosquito tissue

text: mosquito metagenome

text: Cx

text: Culex pipiens/restuans


text: UCSDMI

characteristic: INSDC center name

text: public

text: qiita_sid_10815:10815.1F003OtsW31LAM2012

text: UBERON:multicellular organism

text: UBERON:tissue

text: UBERON:gut

text: Ochlerotatus triseriatus 1F003OtsW31LAM2012

text: TRUE

text: temperate biome

text: insecta-associated habitat

text: organic material

text: host-associated

text: Canada

text: LAM

text: Ochlerotatus triseriatus

text: mosquito

text: mosquito metagenome

text: U Toronto

text: FALSE

text: TRUE

characteristic: public

text: mosquito tissue

text: mosquito metagenome

text: Ots

text: Ochlerotatus triseriatus

characteristic: wnv


text: UCSDMI

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: qiita_sid_10815:10815.1F003SalW31LAM2012

text: UBERON:multicellular organism

text: UBERON:tissue

text: UBERON:gut

text: Culex salinarius 1F003SalW31LAM2012

text: TRUE

text: temperate biome

text: insecta-associated habitat

text: organic material

text: host-associated

text: Canada

text: LAM

text: Culex salinarius

text: mosquito

text: mosquito metagenome

characteristic: organism

text: U Toronto

text: FALSE

text: TRUE

text: mosquito tissue

text: mosquito metagenome

text: Sal

text: Culex salinarius


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UCSDMI

text: public

text: UBERON:multicellular organism

text: UBERON:tissue

text: UBERON:gut

text: Aedes vexans complex 3A019AvW31LAM2012

text: TRUE

text: temperate biome

text: insecta-associated habitat

text: organic material

text: host-associated

text: Canada

text: LAM

text: Aedes vexans complex

text: mosquito

text: mosquito metagenome

text: U Toronto

text: FALSE

text: TRUE

text: mosquito tissue

text: mosquito metagenome

text: Av

text: Aedes vexans complex

characteristic: title


characteristic: ENA-LAST-UPDATE

text: UCSDMI

text: public

characteristic: INSDC status

characteristic: SRA accession

text: UBERON:multicellular organism

text: UBERON:tissue

text: UBERON:gut

text: Ochlerotatus japonicus 3A024OjW33LAM2012

text: TRUE

text: temperate biome

text: insecta-associated habitat

text: organic material

text: host-associated

text: Canada

text: LAM

text: Ochlerotatus japonicus

text: mosquito

text: mosquito metagenome

text: U Toronto

text: FALSE

text: TRUE

text: mosquito tissue

text: mosquito metagenome

text: Oj

text: Ochlerotatus japonicus


characteristic: External Id

text: UCSDMI

text: public

characteristic: Submitter Id

text: UBERON:multicellular organism

text: UBERON:tissue

text: UBERON:gut

text: Aedes vexans complex 3A081AvW32LAM2012

characteristic: description

text: TRUE

text: temperate biome

text: insecta-associated habitat

text: organic material

text: host-associated

text: Canada

text: LAM

text: Aedes vexans complex

text: mosquito

text: mosquito metagenome

text: U Toronto

text: FALSE

text: TRUE

text: mosquito tissue

text: mosquito metagenome

text: Av

text: Aedes vexans complex

text: unknown


text: UCSDMI

text: public

text: UBERON:multicellular organism

text: UBERON:tissue

text: UBERON:gut

text: Culex pipiens/restuans 3A081CxW32LAM2012

text: TRUE

characteristic: elevation

text: temperate biome

text: insecta-associated habitat

text: organic material

text: host-associated

text: Canada

text: LAM

text: Culex pipiens/restuans

characteristic: host scientific name

text: mosquito

characteristic: latitude

text: mosquito metagenome

text: U Toronto

text: FALSE

text: TRUE

text: mosquito tissue

text: mosquito metagenome

text: Cx

text: Culex pipiens/restuans


text: UCSDMI

characteristic: INSDC center name

text: public

text: UBERON:multicellular organism

text: UBERON:tissue

text: UBERON:gut

text: Ochlerotatus japonicus 3A081OjW32LAM2012

text: TRUE

text: temperate biome

text: insecta-associated habitat

text: organic material

text: host-associated

text: Canada

text: LAM

text: Ochlerotatus japonicus

text: mosquito

characteristic: longitude

text: mosquito metagenome

text: U Toronto

text: FALSE

text: TRUE

characteristic: public

text: mosquito tissue

text: mosquito metagenome

text: Oj

text: Ochlerotatus japonicus


text: UCSDMI

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: qiita_sid_10815:10815.3F005AnpW34LAM2012

text: UBERON:multicellular organism

text: UBERON:tissue

text: UBERON:gut

text: Anopheles punctipennis 3F005AnpW34LAM2012

text: TRUE

text: temperate biome

text: insecta-associated habitat

text: organic material

text: host-associated

text: Canada

text: LAM

text: Anopheles punctipennis

text: mosquito

text: mosquito metagenome

text: U Toronto

text: FALSE

text: TRUE

text: mosquito tissue

text: mosquito metagenome

text: Anp

text: Anopheles punctipennis

characteristic: wnv


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UCSDMI

text: public

text: UBERON:multicellular organism

text: UBERON:tissue

text: UBERON:gut

text: Aedes vexans complex 3F005AvW32LAM2012

text: TRUE

text: temperate biome

text: insecta-associated habitat

text: organic material

text: host-associated

text: Canada

text: LAM

text: Aedes vexans complex

text: mosquito

text: mosquito metagenome

characteristic: organism

text: U Toronto

text: FALSE

text: TRUE

text: mosquito tissue

text: mosquito metagenome

text: Av

text: Aedes vexans complex

text: unknown


characteristic: ENA-LAST-UPDATE

text: UCSDMI

text: public

characteristic: INSDC status

text: UBERON:multicellular organism

text: UBERON:tissue

text: UBERON:gut

text: Culex pipiens/restuans 3F005CxW32LAM2012

text: TRUE

text: temperate biome

text: insecta-associated habitat

text: organic material

text: host-associated

text: Canada

text: LAM

text: Culex pipiens/restuans

text: mosquito

text: mosquito metagenome

text: U Toronto

text: FALSE

text: TRUE

text: mosquito tissue

text: mosquito metagenome

text: Cx

text: Culex pipiens/restuans

characteristic: title


characteristic: External Id

text: UCSDMI

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: UBERON:multicellular organism

text: UBERON:tissue

text: UBERON:gut

text: Culex pipiens/restuans 3F005CxW33LAM2012

text: TRUE

text: temperate biome

text: insecta-associated habitat

text: organic material

text: host-associated

text: Canada

text: LAM

text: Culex pipiens/restuans

text: mosquito

text: mosquito metagenome

text: U Toronto

text: FALSE

text: TRUE

text: mosquito tissue

text: mosquito metagenome

text: Cx

text: Culex pipiens/restuans


text: UCSDMI

text: public

text: UBERON:multicellular organism

text: UBERON:tissue

text: UBERON:gut

text: Ochlerotatus japonicus 3F005OjW31LAM2012

characteristic: description

text: TRUE

characteristic: elevation

text: temperate biome

text: insecta-associated habitat

text: organic material

text: host-associated

text: Canada

text: LAM

text: Ochlerotatus japonicus

characteristic: host scientific name

text: mosquito

text: mosquito metagenome

text: U Toronto

text: FALSE

text: TRUE

text: mosquito tissue

text: mosquito metagenome

text: Oj

text: Ochlerotatus japonicus


text: UCSDMI

characteristic: INSDC center name

text: public

text: UBERON:multicellular organism

text: UBERON:tissue

text: UBERON:gut

text: Ochlerotatus japonicus 3F005OjW32LAM2012

text: TRUE

text: temperate biome

text: insecta-associated habitat

text: organic material

text: host-associated

text: Canada

text: LAM

text: Ochlerotatus japonicus

text: mosquito

characteristic: latitude

characteristic: longitude

text: mosquito metagenome

text: U Toronto

text: FALSE

text: TRUE

text: mosquito tissue

text: mosquito metagenome

text: Oj

text: Ochlerotatus japonicus


text: UCSDMI

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: qiita_sid_10815:10815.3F005OtvW33LAM2012

text: UBERON:multicellular organism

text: UBERON:tissue

text: UBERON:gut

text: Ochlerotatus trivitatus 3F005OtvW33LAM2012

text: TRUE

text: temperate biome

text: insecta-associated habitat

text: organic material

text: host-associated

text: Canada

text: LAM

text: Ochlerotatus trivittatus

text: mosquito

text: mosquito metagenome

text: U Toronto

text: FALSE

text: TRUE

characteristic: public

text: mosquito tissue

text: mosquito metagenome

text: Otv

text: Ochlerotatus trivitatus


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UCSDMI

text: public

text: qiita_sid_10815:10815.3F005SalW34LAM2012

text: UBERON:multicellular organism

text: UBERON:tissue

text: UBERON:gut

text: Culex salinarius 3F005SalW34LAM2012

text: TRUE

text: temperate biome

text: insecta-associated habitat

text: organic material

text: host-associated

text: Canada

text: LAM

text: Culex salinarius

text: mosquito

text: mosquito metagenome

text: U Toronto

text: FALSE

text: TRUE

text: mosquito tissue

text: mosquito metagenome

text: Sal

text: Culex salinarius

characteristic: wnv


characteristic: ENA-LAST-UPDATE

text: UCSDMI

text: public

characteristic: INSDC status

text: UBERON:multicellular organism

text: UBERON:tissue

text: UBERON:gut

text: Aedes vexans complex 4A027AvW31LAM2012

text: TRUE

text: temperate biome

text: insecta-associated habitat

text: organic material

text: host-associated

text: Canada

text: LAM

text: Aedes vexans complex

text: mosquito

text: mosquito metagenome

characteristic: organism

text: U Toronto

text: FALSE

text: TRUE

text: mosquito tissue

text: mosquito metagenome

text: Av

text: Aedes vexans complex

text: unknown


characteristic: External Id

text: UCSDMI

text: public

characteristic: SRA accession

text: UBERON:multicellular organism

text: UBERON:tissue

text: UBERON:gut

text: Coquilletidia perturbans 4A027CqW31LAM2012

text: TRUE

text: temperate biome

text: insecta-associated habitat

text: organic material

text: host-associated

text: Canada

text: LAM

text: Coquillettidia perturbans

text: mosquito

text: mosquito metagenome

text: U Toronto

text: FALSE

text: TRUE

text: mosquito tissue

text: mosquito metagenome

text: Cq

text: Coquilletidia perturbans

characteristic: title

text: unknown


text: UCSDMI

text: public

characteristic: Submitter Id

text: UBERON:multicellular organism

text: UBERON:tissue

text: UBERON:gut

text: Aedes vexans complex 4A033AvW32LAM2012

characteristic: description

text: TRUE

characteristic: elevation

text: temperate biome

text: insecta-associated habitat

text: organic material

text: host-associated

text: Canada

text: LAM

text: Aedes vexans complex

text: mosquito

text: mosquito metagenome

text: U Toronto

text: FALSE

text: TRUE

text: mosquito tissue

text: mosquito metagenome

text: Av

text: Aedes vexans complex

text: unknown


text: UCSDMI

characteristic: INSDC center name

text: public

text: UBERON:multicellular organism

text: UBERON:tissue

text: UBERON:gut

text: Culex pipiens/restuans 4A033CxW32LAM2012

text: TRUE

text: temperate biome

text: insecta-associated habitat

text: organic material

text: host-associated

text: Canada

text: LAM

text: Culex pipiens/restuans

characteristic: host scientific name

text: mosquito

characteristic: latitude

text: mosquito metagenome

text: U Toronto

text: FALSE

text: TRUE

text: mosquito tissue

text: mosquito metagenome

text: Cx

text: Culex pipiens/restuans


text: UCSDMI

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: UBERON:multicellular organism

text: UBERON:tissue

text: UBERON:gut

text: Ochlerotatus japonicus 4A033OjW32LAM2012

text: TRUE

text: temperate biome

text: insecta-associated habitat

text: organic material

text: host-associated

text: Canada

text: LAM

text: Ochlerotatus japonicus

text: mosquito

characteristic: longitude

text: mosquito metagenome

text: U Toronto

text: FALSE

text: TRUE

text: mosquito tissue

text: mosquito metagenome

text: Oj

text: Ochlerotatus japonicus


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UCSDMI

text: public

text: qiita_sid_10815:10815.4A067AnqW34LAM2012

text: UBERON:multicellular organism

text: UBERON:tissue

text: UBERON:gut

text: Anopheles quadrimaculatus 4A067AnqW34LAM2012

text: TRUE

text: temperate biome

text: insecta-associated habitat

text: organic material

text: host-associated

text: Canada

text: LAM

text: Anopheles quadrimaculatus

text: mosquito

text: mosquito metagenome

text: U Toronto

text: FALSE

text: TRUE

characteristic: public

text: mosquito tissue

text: mosquito metagenome

text: Anq

text: Anopheles quadrimaculatus


characteristic: ENA-LAST-UPDATE

text: UCSDMI

text: public

characteristic: INSDC status

text: UBERON:multicellular organism

text: UBERON:tissue

text: UBERON:gut

text: Aedes vexans complex 4F001AvW31LAM2012

text: TRUE

text: temperate biome

text: insecta-associated habitat

text: organic material

text: host-associated

text: Canada

text: LAM

text: Aedes vexans complex

text: mosquito

text: mosquito metagenome

text: U Toronto

text: FALSE

text: TRUE

text: mosquito tissue

text: mosquito metagenome

text: Av

text: Aedes vexans complex

text: unknown

characteristic: wnv


characteristic: External Id

text: UCSDMI

text: public

characteristic: SRA accession

text: UBERON:multicellular organism

text: UBERON:tissue

text: UBERON:gut

text: Aedes vexans complex 4F001AvW32LAM2012

text: TRUE

text: temperate biome

text: insecta-associated habitat

text: organic material

text: host-associated

text: Canada

text: LAM

text: Aedes vexans complex

text: mosquito

text: mosquito metagenome

characteristic: organism

text: U Toronto

text: FALSE

text: TRUE

text: mosquito tissue

text: mosquito metagenome

text: Av

text: Aedes vexans complex

text: unknown


text: UCSDMI

text: public

characteristic: Submitter Id

text: UBERON:multicellular organism

text: UBERON:tissue

text: UBERON:gut

text: Coquilletidia perturbans 4F001CqW31LAM2012

characteristic: description

text: TRUE

text: temperate biome

text: insecta-associated habitat

text: organic material

text: host-associated

text: Canada

text: LAM

text: Coquillettidia perturbans

text: mosquito

text: mosquito metagenome

text: U Toronto

text: FALSE

text: TRUE

text: mosquito tissue

text: mosquito metagenome

text: Cq

text: Coquilletidia perturbans

characteristic: title

text: unknown


text: UCSDMI

characteristic: INSDC center name

text: public

text: UBERON:multicellular organism

text: UBERON:tissue

text: UBERON:gut

text: Coquilletidia perturbans 4F001CqW32LAM2012

text: TRUE

characteristic: elevation

text: temperate biome

text: insecta-associated habitat

text: organic material

text: host-associated

text: Canada

text: LAM

text: Coquillettidia perturbans

characteristic: host scientific name

text: mosquito

text: mosquito metagenome

text: U Toronto

text: FALSE

text: TRUE

text: mosquito tissue

text: mosquito metagenome

text: Cq

text: Coquilletidia perturbans

text: unknown


text: UCSDMI

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: UBERON:multicellular organism

text: UBERON:tissue

text: UBERON:gut

text: Coquilletidia perturbans 4F001CqW33LAM2012

text: TRUE

text: temperate biome

text: insecta-associated habitat

text: organic material

text: host-associated

text: Canada

text: LAM

text: Coquillettidia perturbans

text: mosquito

characteristic: latitude

text: mosquito metagenome

text: U Toronto

text: FALSE

text: TRUE

text: mosquito tissue

text: mosquito metagenome

text: Cq

text: Coquilletidia perturbans

text: unknown


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UCSDMI

text: public

text: UBERON:multicellular organism

text: UBERON:tissue

text: UBERON:gut

text: Culex pipiens/restuans 4F001CxW32LAM2012

text: TRUE

text: temperate biome

text: insecta-associated habitat

text: organic material

text: host-associated

text: Canada

text: LAM

text: Culex pipiens/restuans

text: mosquito

characteristic: longitude

text: mosquito metagenome

text: U Toronto

text: FALSE

text: TRUE

text: mosquito tissue

text: mosquito metagenome

text: Cx

text: Culex pipiens/restuans


characteristic: ENA-LAST-UPDATE

text: UCSDMI

text: public

characteristic: INSDC status

text: UBERON:multicellular organism

text: UBERON:tissue

text: UBERON:gut

text: Culex pipiens/restuans 4F001CxW34LAM2012

text: TRUE

text: temperate biome

text: insecta-associated habitat

text: organic material

text: host-associated

text: Canada

text: LAM

text: Culex pipiens/restuans

text: mosquito

text: mosquito metagenome

text: U Toronto

text: FALSE

text: TRUE

characteristic: public

text: mosquito tissue

text: mosquito metagenome

text: Cx

text: Culex pipiens/restuans


characteristic: External Id

text: UCSDMI

text: public

characteristic: SRA accession

text: UBERON:multicellular organism

text: UBERON:tissue

text: UBERON:gut

text: Ochlerotatus japonicus 4F001OjW34LAM2012

text: TRUE

text: temperate biome

text: insecta-associated habitat

text: organic material

text: host-associated

text: Canada

text: LAM

text: Ochlerotatus japonicus

text: mosquito

text: mosquito metagenome

text: U Toronto

text: FALSE

text: TRUE

text: mosquito tissue

text: mosquito metagenome

text: Oj

text: Ochlerotatus japonicus

characteristic: wnv


text: UCSDMI

text: public

characteristic: Submitter Id

text: UBERON:multicellular organism

text: UBERON:tissue

text: UBERON:gut

text: Coquilletidia perturbans 6A038CqW31LAM2012

characteristic: description

text: TRUE

text: temperate biome

text: insecta-associated habitat

text: organic material

text: host-associated

text: Canada

text: LAM

text: Coquillettidia perturbans

text: mosquito

text: mosquito metagenome

characteristic: organism

text: U Toronto

text: FALSE

text: TRUE

text: mosquito tissue

text: mosquito metagenome

text: Cq

text: Coquilletidia perturbans

text: unknown


text: UCSDMI

characteristic: INSDC center name

text: public

text: UBERON:multicellular organism

text: UBERON:tissue

text: UBERON:gut

text: Coquilletidia perturbans 6A060CqW31LAM2012

text: TRUE

characteristic: elevation

text: temperate biome

text: insecta-associated habitat

text: organic material

text: host-associated

text: Canada

text: LAM

text: Coquillettidia perturbans

text: mosquito

text: mosquito metagenome

text: U Toronto

text: FALSE

text: TRUE

text: mosquito tissue

text: mosquito metagenome

text: Cq

text: Coquilletidia perturbans

characteristic: title

text: unknown


text: UCSDMI

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: UBERON:multicellular organism

text: UBERON:tissue

text: UBERON:gut

text: Coquilletidia perturbans 6A073CqW33LAM2012

text: TRUE

text: temperate biome

text: insecta-associated habitat

text: organic material

text: host-associated

text: Canada

text: LAM

text: Coquillettidia perturbans

characteristic: host scientific name

text: mosquito

text: mosquito metagenome

text: U Toronto

text: FALSE

text: TRUE

text: mosquito tissue

text: mosquito metagenome

text: Cq

text: Coquilletidia perturbans

text: unknown


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UCSDMI

text: public

text: UBERON:multicellular organism

text: UBERON:tissue

text: UBERON:gut

text: Aedes vexans complex 6A082AvW32LAM2012

text: TRUE

text: temperate biome

text: insecta-associated habitat

text: organic material

text: host-associated

text: Canada

text: LAM

text: Aedes vexans complex

text: mosquito

characteristic: latitude

text: mosquito metagenome

text: U Toronto

text: FALSE

text: TRUE

text: mosquito tissue

text: mosquito metagenome

text: Av

text: Aedes vexans complex

text: unknown


characteristic: ENA-LAST-UPDATE

text: UCSDMI

text: public

characteristic: INSDC status

text: UBERON:multicellular organism

text: UBERON:tissue

text: UBERON:gut

text: Coquilletidia perturbans 6A082CqW32LAM2012

text: TRUE

text: temperate biome

text: insecta-associated habitat

text: organic material

text: host-associated

text: Canada

text: LAM

text: Coquillettidia perturbans

text: mosquito

characteristic: longitude

text: mosquito metagenome

text: U Toronto

text: FALSE

text: TRUE

text: mosquito tissue

text: mosquito metagenome

text: Cq

text: Coquilletidia perturbans

text: unknown


characteristic: External Id

text: UCSDMI

text: public

characteristic: SRA accession

text: UBERON:multicellular organism

text: UBERON:tissue

text: UBERON:gut

text: Culex pipiens/restuans 6A082CxW32LAM2012

text: TRUE

text: temperate biome

text: insecta-associated habitat

text: organic material

text: host-associated

text: Canada

text: LAM

text: Culex pipiens/restuans

text: mosquito

text: mosquito metagenome

text: U Toronto

text: FALSE

text: TRUE

characteristic: public

text: mosquito tissue

text: mosquito metagenome

text: Cx

text: Culex pipiens/restuans


text: UCSDMI

text: public

characteristic: Submitter Id

text: UBERON:multicellular organism

text: UBERON:tissue

text: UBERON:gut

text: Aedes vexans complex 6F002AvW33LAM2012

characteristic: description

text: TRUE

text: temperate biome

text: insecta-associated habitat

text: organic material

text: host-associated

text: Canada

text: LAM

text: Aedes vexans complex

text: mosquito

text: mosquito metagenome

text: U Toronto

text: FALSE

text: TRUE

text: mosquito tissue

text: mosquito metagenome

text: Av

text: Aedes vexans complex

text: unknown

characteristic: wnv


text: UCSDMI

characteristic: INSDC center name

text: public

text: UBERON:multicellular organism

text: UBERON:tissue

text: UBERON:gut

text: Culex pipiens/restuans 6F002CxW32LAM2012

text: TRUE

characteristic: elevation

text: temperate biome

text: insecta-associated habitat

text: organic material

text: host-associated

text: Canada

text: LAM

text: Culex pipiens/restuans

text: mosquito

text: mosquito metagenome

characteristic: organism

text: U Toronto

text: FALSE

text: TRUE

text: mosquito tissue

text: mosquito metagenome

text: Cx

text: Culex pipiens/restuans


text: UCSDMI

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: UBERON:multicellular organism

text: UBERON:tissue

text: UBERON:gut

text: Culex pipiens/restuans 6F002CxW33LAM2012

text: TRUE

text: temperate biome

text: insecta-associated habitat

text: organic material

text: host-associated

text: Canada

text: LAM

text: Culex pipiens/restuans

text: mosquito

text: mosquito metagenome

text: U Toronto

text: FALSE

text: TRUE

text: mosquito tissue

text: mosquito metagenome

text: Cx

text: Culex pipiens/restuans

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UCSDMI

text: public

text: UBERON:multicellular organism

text: UBERON:tissue

text: UBERON:gut

text: Culex pipiens/restuans 6F002CxW34LAM2012

text: TRUE

text: temperate biome

text: insecta-associated habitat

text: organic material

text: host-associated

text: Canada

text: LAM

text: Culex pipiens/restuans

characteristic: host scientific name

text: mosquito

text: mosquito metagenome

text: U Toronto

text: FALSE

text: TRUE

text: mosquito tissue

text: mosquito metagenome

text: Cx

text: Culex pipiens/restuans


characteristic: ENA-LAST-UPDATE

text: UCSDMI

text: public

characteristic: INSDC status

text: qiita_sid_10815:10815.6F002OcanW33LAM2012

text: UBERON:multicellular organism

text: UBERON:tissue

text: UBERON:gut

text: Ochlerotatus canadensis 6F002OcanW33LAM2012

text: TRUE

text: temperate biome

text: insecta-associated habitat

text: organic material

text: host-associated

text: Canada

text: LAM

text: Ochlerotatus canadensis

text: mosquito

characteristic: latitude

text: mosquito metagenome

text: U Toronto

text: FALSE

text: TRUE

text: mosquito tissue

text: mosquito metagenome

text: Ocan

text: Ochlerotatus canadensis

text: unknown


characteristic: External Id

text: UCSDMI

text: public

characteristic: SRA accession

text: UBERON:multicellular organism

text: UBERON:tissue

text: UBERON:gut

text: Aedes vexans complex 7A050AvW31LAM2012

text: TRUE

text: temperate biome

text: insecta-associated habitat

text: organic material

text: host-associated

text: Canada

text: LAM

text: Aedes vexans complex

text: mosquito

characteristic: longitude

text: mosquito metagenome

text: U Toronto

text: FALSE

text: TRUE

text: mosquito tissue

text: mosquito metagenome

text: Av

text: Aedes vexans complex

text: unknown


text: UCSDMI

text: public

characteristic: Submitter Id

text: UBERON:multicellular organism

text: UBERON:tissue

text: UBERON:gut

text: Culex pipiens/restuans 7A050CxW31LAM2012

characteristic: description

text: TRUE

text: temperate biome

text: insecta-associated habitat

text: organic material

text: host-associated

text: Canada

text: LAM

text: Culex pipiens/restuans

text: mosquito

text: mosquito metagenome

text: U Toronto

text: FALSE

text: TRUE

characteristic: public

text: mosquito tissue

text: mosquito metagenome

text: Cx

text: Culex pipiens/restuans


text: UCSDMI

characteristic: INSDC center name

text: public

text: qiita_sid_10815:10815.7A050SalW31LAM2012

text: UBERON:multicellular organism

text: UBERON:tissue

text: UBERON:gut

text: Culex salinarius 7A050SalW31LAM2012

text: TRUE

characteristic: elevation

text: temperate biome

text: insecta-associated habitat

text: organic material

text: host-associated

text: Canada

text: LAM

text: Culex salinarius

text: mosquito

text: mosquito metagenome

text: U Toronto

text: FALSE

text: TRUE

text: mosquito tissue

text: mosquito metagenome

text: Sal

text: Culex salinarius

characteristic: wnv


text: UCSDMI

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: UBERON:multicellular organism

text: UBERON:tissue

text: UBERON:gut

text: Aedes vexans complex 7F004AvW31LAM2012

text: TRUE

text: temperate biome

text: insecta-associated habitat

text: organic material

text: host-associated

text: Canada

text: LAM

text: Aedes vexans complex

text: mosquito

text: mosquito metagenome

characteristic: organism

text: U Toronto

text: FALSE

text: TRUE

text: mosquito tissue

text: mosquito metagenome

text: Av

text: Aedes vexans complex

text: unknown


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UCSDMI

text: public

text: UBERON:multicellular organism

text: UBERON:tissue

text: UBERON:gut

text: Aedes vexans complex 7F004AvW32LAM2012

text: TRUE

text: temperate biome

text: insecta-associated habitat

text: organic material

text: host-associated

text: Canada

text: LAM

text: Aedes vexans complex

text: mosquito

text: mosquito metagenome

text: U Toronto

text: FALSE

text: TRUE

text: mosquito tissue

text: mosquito metagenome

text: Av

text: Aedes vexans complex

characteristic: title

text: unknown


characteristic: ENA-LAST-UPDATE

text: UCSDMI

text: public

characteristic: INSDC status

text: UBERON:multicellular organism

text: UBERON:tissue

text: UBERON:gut

text: Aedes vexans complex 7F004AvW33LAM2012

text: TRUE

text: temperate biome

text: insecta-associated habitat

text: organic material

text: host-associated

text: Canada

text: LAM

text: Aedes vexans complex

characteristic: host scientific name

text: mosquito

text: mosquito metagenome

text: U Toronto

text: FALSE

text: TRUE

text: mosquito tissue

text: mosquito metagenome

text: Av

text: Aedes vexans complex

text: unknown


characteristic: External Id

text: UCSDMI

text: public

characteristic: SRA accession

text: UBERON:multicellular organism

text: UBERON:tissue

text: UBERON:gut

text: Aedes vexans complex 7F004AvW34LAM2012

text: TRUE

text: temperate biome

text: insecta-associated habitat

text: organic material

text: host-associated

text: Canada

text: LAM

text: Aedes vexans complex

text: mosquito

characteristic: latitude

text: mosquito metagenome

text: U Toronto

text: FALSE

text: TRUE

text: mosquito tissue

text: mosquito metagenome

text: Av

text: Aedes vexans complex

text: unknown


text: UCSDMI

text: public

characteristic: Submitter Id

text: UBERON:multicellular organism

text: UBERON:tissue

text: UBERON:gut

text: Coquilletidia perturbans 7F004CqW31LAM2012

characteristic: description

text: TRUE

text: temperate biome

text: insecta-associated habitat

text: organic material

text: host-associated

text: Canada

text: LAM

text: Coquillettidia perturbans

text: mosquito

characteristic: longitude

text: mosquito metagenome

text: U Toronto

text: FALSE

text: TRUE

text: mosquito tissue

text: mosquito metagenome

text: Cq

text: Coquilletidia perturbans

text: unknown


text: UCSDMI

characteristic: INSDC center name

text: public

text: UBERON:multicellular organism

text: UBERON:tissue

text: UBERON:gut

text: Coquilletidia perturbans 7F004CqW32LAM2012

text: TRUE

characteristic: elevation

text: temperate biome

text: insecta-associated habitat

text: organic material

text: host-associated

text: Canada

text: LAM

text: Coquillettidia perturbans

text: mosquito

text: mosquito metagenome

text: U Toronto

text: FALSE

text: TRUE

characteristic: public

text: mosquito tissue

text: mosquito metagenome

text: Cq

text: Coquilletidia perturbans

text: unknown


text: UCSDMI

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: UBERON:multicellular organism

text: UBERON:tissue

text: UBERON:gut

text: Coquilletidia perturbans 7F004CqW33LAM2012

text: TRUE

text: temperate biome

text: insecta-associated habitat

text: organic material

text: host-associated

text: Canada

text: LAM

text: Coquillettidia perturbans

text: mosquito

text: mosquito metagenome

text: U Toronto

text: FALSE

text: TRUE

text: mosquito tissue

text: mosquito metagenome

text: Cq

text: Coquilletidia perturbans

text: unknown

characteristic: wnv


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UCSDMI

text: public

text: UBERON:multicellular organism

text: UBERON:tissue

text: UBERON:gut

text: Culex pipiens/restuans 7F004CxW31LAM2012

text: TRUE

text: temperate biome

text: insecta-associated habitat

text: organic material

text: host-associated

text: Canada

text: LAM

text: Culex pipiens/restuans

text: mosquito

text: mosquito metagenome

characteristic: organism

text: U Toronto

text: FALSE

text: TRUE

text: mosquito tissue

text: mosquito metagenome

text: Cx

text: Culex pipiens/restuans


characteristic: ENA-LAST-UPDATE

text: UCSDMI

text: public

characteristic: INSDC status

text: UBERON:multicellular organism

text: UBERON:tissue

text: UBERON:gut

text: Culex pipiens/restuans 7F004CxW32LAM2012

text: TRUE

text: temperate biome

text: insecta-associated habitat

text: organic material

text: host-associated

text: Canada

text: LAM

text: Culex pipiens/restuans

text: mosquito

text: mosquito metagenome

text: U Toronto

text: FALSE

text: TRUE

text: mosquito tissue

text: mosquito metagenome

text: Cx

text: Culex pipiens/restuans

characteristic: title


characteristic: External Id

text: UCSDMI

text: public

characteristic: SRA accession

text: UBERON:multicellular organism

text: UBERON:tissue

text: UBERON:gut

text: Culex pipiens/restuans 7F004CxW33LAM2012

text: TRUE

text: temperate biome

text: insecta-associated habitat

text: organic material

text: host-associated

text: Canada

text: LAM

text: Culex pipiens/restuans

characteristic: host scientific name

text: mosquito

text: mosquito metagenome

text: U Toronto

text: FALSE

text: TRUE

text: mosquito tissue

text: mosquito metagenome

text: Cx

text: Culex pipiens/restuans


text: UCSDMI

text: public

characteristic: Submitter Id

text: UBERON:multicellular organism

text: UBERON:tissue

text: UBERON:gut

text: Culex pipiens/restuans 7F004CxW34LAM2012

characteristic: description

text: TRUE

text: temperate biome

text: insecta-associated habitat

text: organic material

text: host-associated

text: Canada

text: LAM

text: Culex pipiens/restuans

text: mosquito

characteristic: latitude

text: mosquito metagenome

text: U Toronto

text: FALSE

text: TRUE

text: mosquito tissue

text: mosquito metagenome

text: Cx

text: Culex pipiens/restuans


text: UCSDMI

characteristic: INSDC center name

text: public

text: UBERON:multicellular organism

text: UBERON:tissue

text: UBERON:gut

text: Ochlerotatus japonicus 7F004OjW34LAM2012

text: TRUE

characteristic: elevation

text: temperate biome

text: insecta-associated habitat

text: organic material

text: host-associated

text: Canada

text: LAM

text: Ochlerotatus japonicus

text: mosquito

characteristic: longitude

text: mosquito metagenome

text: U Toronto

text: FALSE

text: TRUE

text: mosquito tissue

text: mosquito metagenome

text: Oj

text: Ochlerotatus japonicus


text: UCSDMI

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: UBERON:multicellular organism

text: UBERON:tissue

text: UBERON:gut

text: Coquilletidia perturbans 8A063CqW32LAM2012

text: TRUE

text: temperate biome

text: insecta-associated habitat

text: organic material

text: host-associated

text: Canada

text: LAM

text: Coquillettidia perturbans

text: mosquito

text: mosquito metagenome

text: U Toronto

text: FALSE

text: TRUE

characteristic: public

text: mosquito tissue

text: mosquito metagenome

text: Cq

text: Coquilletidia perturbans

text: unknown


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: UCSDMI

text: public

text: UBERON:multicellular organism

text: UBERON:tissue

text: UBERON:gut

text: Aedes vexans complex 8A079AvW34LAM2012

text: TRUE

text: temperate biome

text: insecta-associated habitat

text: organic material

text: host-associated

text: Canada

text: LAM

text: Aedes vexans complex

text: mosquito

text: mosquito metagenome

text: U Toronto

text: FALSE

text: TRUE

text: mosquito tissue

text: mosquito metagenome

text: Av

text: Aedes vexans complex

characteristic: wnv


characteristic: ENA-LAST-UPDATE

text: UCSDMI

text: public

characteristic: INSDC status

text: UBERON:multicellular organism

text: UBERON:tissue

text: UBERON:gut

text: Aedes vexans complex 9F006AvW34LAM2012

text: TRUE

text: temperate biome

text: insecta-associated habitat

text: organic material

text: host-associated

text: Canada

text: LAM

text: Aedes vexans complex

text: mosquito

text: mosquito metagenome

characteristic: organism

text: U Toronto

text: FALSE

text: TRUE

text: mosquito tissue

text: mosquito metagenome

text: Av

text: Aedes vexans complex


characteristic: External Id

text: UCSDMI

text: public

characteristic: SRA accession

text: UBERON:multicellular organism

text: UBERON:tissue

text: UBERON:gut

text: Culex pipiens/restuans 9F006CxW33LAM2012

text: TRUE

text: temperate biome

text: insecta-associated habitat

text: organic material

text: host-associated

text: Canada

text: LAM

text: Culex pipiens/restuans

text: mosquito

text: mosquito metagenome

text: U Toronto

text: FALSE

text: TRUE

text: mosquito tissue

text: mosquito metagenome

text: Cx

text: Culex pipiens/restuans

characteristic: title


text: UCSDMI

text: public

characteristic: Submitter Id

text: UBERON:multicellular organism

text: UBERON:tissue

text: UBERON:gut

text: Anopheles punctipennis A1AnpW34TOR2012

characteristic: description

text: TRUE

text: temperate biome

text: insecta-associated habitat

text: organic material

text: host-associated

text: Canada

text: TOR

text: Anopheles punctipennis

characteristic: host scientific name

text: mosquito

text: mosquito metagenome

text: U Toronto

text: FALSE

text: TRUE

text: mosquito tissue

text: mosquito metagenome

text: Anp

text: Anopheles punctipennis