text: King Fahd Medical Research Centre

text: public

text: Fermented Food

text: food fermentation metagenome

text: food fermentation metagenome

text: Hungarain Feta cheese


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: King Fahd Medical Research Centre

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:King Fahd Medical Research Centre

characteristic: Submitter Id

text: Fermented Food

characteristic: description

text: food fermentation metagenome

characteristic: organism

text: food fermentation metagenome

text: Syrain Cheese

characteristic: title


text: King Fahd Medical Research Centre

text: public

text: Fermented Food

text: food fermentation metagenome

text: food fermentation metagenome

text: Egyption Cheese


text: King Fahd Medical Research Centre

text: public

text: Fermented Food

text: food fermentation metagenome

text: food fermentation metagenome

text: Mixed pickles vegetables


text: King Fahd Medical Research Centre

text: public

text: Fermented Food

text: food fermentation metagenome

text: food fermentation metagenome

text: Hot Pickles Hijazi


text: King Fahd Medical Research Centre

text: public

text: Fermented Food

text: food fermentation metagenome

text: food fermentation metagenome

text: Egyptian Mish 1


text: King Fahd Medical Research Centre

text: public

text: Fermented Food

text: food fermentation metagenome

text: food fermentation metagenome

text: Egyptian Mish 2


text: King Fahd Medical Research Centre

text: public

text: Fermented Food

text: food fermentation metagenome

text: food fermentation metagenome

text: Egyptian Mish 3


LB-A

text: King Fahd Medical Research Centre

text: public

text: LB-A

text: Fermented Food

text: food fermentation metagenome

text: food fermentation metagenome

text: Home made Laban 1


LB-B

text: King Fahd Medical Research Centre

text: public

text: LB-B

text: Fermented Food

text: food fermentation metagenome

text: food fermentation metagenome

text: Home made Laban 2


LB-C

text: King Fahd Medical Research Centre

text: public

text: LB-C

text: Fermented Food

text: food fermentation metagenome

text: food fermentation metagenome

text: Home made Laban 3


text: CENTRE FOR GENOMIC RESEARCH (CGR)

text: public

tag: Namespace:CENTRE FOR GENOMIC RESEARCH (CGR)

text: Sodalis glossinidius str. 'morsitans'

text: Sodalis glossinidius str. 'morsitans'

text: Sodalis glossinidius morsitans Axenic RNAseq


text: Wellcome Trust Sanger Institute

text: public

tag: Namespace:Wellcome Trust Sanger Institute

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: mouse embryonic stem cell

characteristic: cell type

text: house mouse

characteristic: common name

text: Protocols: Wildtype E14 mESC were cultured under standard culture conditions in Knockout DMEM containing 15% Fetal Calf Serum, 1x Penicillin-Streptomycin-Glutamine, 1x NEAA 2-mercaptoethanol and 1000U Leukemia Inhibitory Factor. Trypisnised single mESC suspension is passed through 30micron filter to remove and clumps. A 96-well plate containing lysis buffer (0.2% Triton, 1:20 RNAse inbhibitor, 10mM Oligo-dT30VN, 10mM dNTPs) was prepared. Spike-ins (ERCCs and SIRVs; final 1:1000,000 dilution) are subjected to different freeze-thaw cycles (seven conditions) and 1µl of spike-in mix per condition was added to each well column-wise. Single mES cells were FACS sorted (BD Influx; BD Biosciences) to each well (along with bulk controls). Subsequent steps (Lysis, RT, PCR amplification and DNA purification) was performed as described in Smart-Seq2 publication (Picelli et al Nature Protocol 2014). The single cell cDNA across the 96-well plate was quantified, diluted and libraries were made using Nextera XT DNA sample preparation kit (Illumina) utilising 96 dual barcoded indices. Library pooling was performed post AMPure XP beads (Agencourt Biosciences) clean up and sequenced on HiSeq2500 (v4) using paired end sequencing (75 or 125bp) at the Wellcome Trust Sequencing facility following Wellcome Trust Sequencing guidelines.

text: Serum + LIF condition

characteristic: growth condition

text: Mus musculus

text: Mus musculus

text: Mus musculus

characteristic: strain

type: has member


Bulk

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: JIC

text: public

text: Bulk

text: thale cress

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: F3 seed bulked sample homozygous for awe1-1


mutant

characteristic: External Id

text: JIC

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: mutant

text: thale cress

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: awe1-1 mutant line


text: Imperial College London

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:Imperial College London

text: ArrayExpress

text: western European house mouse

text: Protocols: Sciatic nerves of adult mice were cut or crushed at the sciatic notch. DNA was isolated from samples using phenol chloroform method. Libraries were prepared without pooling to generate methylation profiles on the HiSeq 2000 platform to generate 100bp pair end reads as described in (Johnson et al 2012: DOI: 10.1002/0471142727.mb2123s99)

text: adult

characteristic: developmental stage

text: Mus musculus domesticus

text: Mus musculus domesticus

text: sciatic nerve

characteristic: organism part

text: Mus musculus domesticus


text: Imperial College London

text: public

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: western European house mouse

text: Protocols: No injury was performed on control samples. Sciatic nerves of adult mice were harvested at the sinotic notch. DNA was isolated from samples using phenol chloroform method. Libraries were prepared without pooling to generate methylation profiles on the HiSeq 2000 platform to generate 100bp pair end reads as described in (Johnson et al 2012: DOI: 10.1002/0471142727.mb2123s99)

characteristic: description

text: adult

text: Mus musculus domesticus

characteristic: organism

text: Mus musculus domesticus

text: sciatic nerve

text: Mus musculus domesticus

characteristic: title


text: Imperial College London

text: public

text: ArrayExpress

text: western European house mouse

characteristic: common name

text: Protocols: Sciatic nerves of adult mice were cut or crushed at the sciatic notch. RNA was extracted from samples using Trizol (Invitrogen) according to manufacturer’s instructions with an additional purification step by on-column DNase treatment using the RNase-free DNase Kit (Qiagen) to ensure elimination of any genomic DNA. The integrity and quantity of RNA was determined using Qubit (Invitrogen) and Agilent 2100 Bioanalyzer (Agilent Technologies). Small RNA libraries were prepared using TruSeq Small RNA sample preparation kit (Illumina). Briefly, 3` and 5` indexed adapters were ligated to 1 g of total RNA and the product of this reaction was RT-PCR amplified. Small RNA library was size selected using Pippin Prep elution system (Sage Science). The quality of the libraries was evaluated using Agilent 2100 Bioanalyzer. All the libraries were pooled together and run on two lanes of the HiSeq 2000 platform to generate 50 bp single-end reads.

text: adult

text: Mus musculus domesticus

text: Mus musculus domesticus

text: sciatic nerve

text: Mus musculus domesticus

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: Imperial College London

text: public

text: ArrayExpress

text: western European house mouse

text: Protocols: No injury was performed on control samples. Sciatic nerves of adult mice were harvested at the sinotic notch. RNA was extracted from samples using Trizol (Invitrogen) according to manufacturer’s instructions with an additional purification step by on-column DNase treatment using the RNase-free DNase Kit (Qiagen) to ensure elimination of any genomic DNA. The integrity and quantity of RNA was determined using Qubit (Invitrogen) and Agilent 2100 Bioanalyzer (Agilent Technologies). Small RNA libraries were prepared using TruSeq Small RNA sample preparation kit (Illumina). Briefly, 3` and 5` indexed adapters were ligated to 1 g of total RNA and the product of this reaction was RT-PCR amplified. Small RNA library was size selected using Pippin Prep elution system (Sage Science). The quality of the libraries was evaluated using Agilent 2100 Bioanalyzer. All the libraries were pooled together and run on two lanes of the HiSeq 2000 platform to generate 50 bp single-end reads.

text: adult

text: Mus musculus domesticus

text: Mus musculus domesticus

text: sciatic nerve

text: Mus musculus domesticus


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Imperial College London

text: public

text: ArrayExpress

text: western European house mouse

text: Protocols: Sciatic nerves of adult mice were cut or crushed at the sciatic notch. RNA was extracted from samples using Trizol (Invitrogen) according to manufacturer’s instructions with an additional purification step by on-column DNase treatment using the RNase-free DNase Kit (Qiagen) to ensure elimination of any genomic DNA. The integrity and quantity of RNA was determined using Qubit (Invitrogen) and Agilent 2100 Bioanalyzer (Agilent Technologies). 1 µg of total RNA was used to generate RNA-Seq libraries using TruSeq Stranded total RNA sample preparation kit (Illumina) according to the manufacturer’s instructions. Briefly, cytoplasmic and mitochondrial rRNA was removed using biotinylated, target specific oligos combined with Ribo-Zero rRNA removal beads. Following purification, RNA was fragmented using divalent cations under elevated temperature and cleaved fragments were copied onto first strand cDNA using reverse transcriptase and random primers. Next, the second strand was synthesised using DNA Polymersae I and RNase H. cDNA 3’ ends were adenylated and indexed adapters ligated followed by 10 cycles of library amplification. Finally, the libraries were purified using AMPue XP Beads (Beckman Coulter) and quality was evaluated using Agilent 2100 Bioanalyzer. Three libraries were run on a single lane of the HiSeq 2000 platform (Illumina) to generate 100 bp paired-end reads.

text: adult

text: Mus musculus domesticus

text: Mus musculus domesticus

text: sciatic nerve

text: Mus musculus domesticus


text: Imperial College London

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: ArrayExpress

text: western European house mouse

text: Protocols: No injury was performed on control samples. Sciatic nerves of adult mice were harvested at the sinotic notch. RNA was extracted from samples using Trizol (Invitrogen) according to manufacturer’s instructions with an additional purification step by on-column DNase treatment using the RNase-free DNase Kit (Qiagen) to ensure elimination of any genomic DNA. The integrity and quantity of RNA was determined using Qubit (Invitrogen) and Agilent 2100 Bioanalyzer (Agilent Technologies). 1 µg of total RNA was used to generate RNA-Seq libraries using TruSeq Stranded total RNA sample preparation kit (Illumina) according to the manufacturer’s instructions. Briefly, cytoplasmic and mitochondrial rRNA was removed using biotinylated, target specific oligos combined with Ribo-Zero rRNA removal beads. Following purification, RNA was fragmented using divalent cations under elevated temperature and cleaved fragments were copied onto first strand cDNA using reverse transcriptase and random primers. Next, the second strand was synthesised using DNA Polymersae I and RNase H. cDNA 3’ ends were adenylated and indexed adapters ligated followed by 10 cycles of library amplification. Finally, the libraries were purified using AMPue XP Beads (Beckman Coulter) and quality was evaluated using Agilent 2100 Bioanalyzer. Three libraries were run on a single lane of the HiSeq 2000 platform (Illumina) to generate 100 bp paired-end reads.

text: adult

text: Mus musculus domesticus

text: Mus musculus domesticus

text: sciatic nerve

text: Mus musculus domesticus


text: Wellcome Trust Sanger Institute

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: ArrayExpress

characteristic: cell line

text: mouse embryonic stem cell

characteristic: cell type

text: house mouse

text: Protocols: Wildtype E14 mESC were cultured under standard culture conditions in Knockout DMEM containing 15% Fetal Calf Serum, 1x Penicillin-Streptomycin-Glutamine, 1x NEAA 2-mercaptoethanol and 1000U Leukemia Inhibitory Factor. Trypisnised single mESC suspension is passed through 30micron filter to remove and clumps. Scripts with different method workflows were downloaded from Fluidigm Script Hub. Approx 3000 single mESC are loaded onto 5-10 micron Fluidigm C1 Single-Cell Auto Prep IFC and cell capture was performed as per manufacturers protocol. Two kinds of spike-ins (ERCC-#4456740 and SIRV-E2 #SKU025.03) were mixed (0.5µl 1:500 diluted ERCCs + 0.6µl 1:500 diluted SIRVs) and added to respective Lysis buffer. The subsequent steps are performed as per manufacturers protocol or described on Fluidigm Script hub. The cDNA from each single chamber within C1-IFC was harvested and diluted as per manufacturer protocol. Harvested cDNA from single cells was quantified and libraries were made using Nextera XT DNA sample preparation kit (Illumina) utilising 96 dual barcoded indices. Library pooling was performed post AMPure XP beads (Agencourt Biosciences) clean up and sequenced on HiSeq2500 (v4) using paired end sequencing (75 or 125bp) at the Wellcome Trust Sequencing facility following Wellcome Trust Sequencing guidelines.

text: Serum + LIF condition

characteristic: growth condition

text: Mus musculus

text: Mus musculus

text: Mus musculus


text: public

characteristic: SRA accession

tag: Namespace:NATIONAL HEART AND LUNG INSTITUTE, IMPERIAL COLLEGE LONDON

characteristic: Submitter Id

text: AATGATACGGCGACCACCGAGATCTACAC

characteristic: adapters

characteristic: collection date

text: Patient 29 blind brush

text: Human

characteristic: environment (biome)

text: Lung

characteristic: environment (feature)

text: Mucus

characteristic: environment (material)

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: 51.5074� N

unit: DD

characteristic: geographic location (latitude)

text: 0.1278� W

characteristic: geographic location (longitude)

unit: year

characteristic: host age

text: Child

characteristic: host family relationship

text: Patient 29 blind brush

characteristic: host subject id

text: human-associated

characteristic: human associated environmental package

text: metagenome

characteristic: investigation type

text: Cuthbertson et al 2017

characteristic: library construction method

text: PBB

characteristic: lung/pulmory disorder

text: CGAGGCTGTATCCTCT

characteristic: multiplex identifiers

text: Cuthbertson et al 2017

characteristic: nucleic acid amplification

text: MPBio FastDNATM spin kit for soil

characteristic: nucleic acid extraction

text: human lung metagenome

text: 520F, 802R

characteristic: pcr primers

text: The Impact of Persistent Bacterial Bronchitis on the Pulmonary Microbiome of Children.

characteristic: project name

text: Lung brush

characteristic: sample collection device or method

unit: °C

characteristic: sample storage temperature

unit: mL

characteristic: sample volume or weight for DNA extraction

text: human lung metagenome

text: Illumina Miseq

characteristic: sequencing method

text: 16S rRNA gene

characteristic: target gene


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NATIONAL HEART AND LUNG INSTITUTE, IMPERIAL COLLEGE LONDON

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: AATGATACGGCGACCACCGAGATCTACAC

characteristic: adapters

characteristic: collection date

text: Patient 7 blind brush

characteristic: description

text: Human

characteristic: environment (biome)

text: Lung

characteristic: environment (feature)

text: Mucus

characteristic: environment (material)

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: 51.5074� N

characteristic: geographic location (latitude)

text: 0.1278� W

characteristic: geographic location (longitude)

characteristic: host age

text: Child

text: Patient 7 blind brush

text: human-associated

text: metagenome

text: Cuthbertson et al 2017

text: PBB

text: AAGAGGCAAAGGAGTA

text: Cuthbertson et al 2017

text: MPBio FastDNATM spin kit for soil

text: human lung metagenome

characteristic: organism

text: 520F, 802R

text: Lung brush

text: human lung metagenome

text: Illumina Miseq

text: 16S rRNA gene

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: AATGATACGGCGACCACCGAGATCTACAC

text: Patient 19 blind brush 1

text: Human

text: Lung

text: Mucus

text: United Kingdom

text: 51.5074� N

text: 0.1278� W

text: Child

characteristic: host family relationship

text: Patient 19 blind brush 1

characteristic: host subject id

text: human-associated

characteristic: human associated environmental package

text: metagenome

characteristic: investigation type

text: Cuthbertson et al 2017

characteristic: library construction method

text: CTCTCTACAAGGAGTA

characteristic: multiplex identifiers

text: Cuthbertson et al 2017

characteristic: nucleic acid amplification

text: MPBio FastDNATM spin kit for soil

characteristic: nucleic acid extraction

text: human lung metagenome

text: 520F, 802R

characteristic: pcr primers

characteristic: project name

text: Lung brush

characteristic: sample collection device or method

characteristic: sample storage temperature

characteristic: sample volume or weight for DNA extraction

text: human lung metagenome

text: Illumina Miseq

characteristic: sequencing method

text: 16S rRNA gene

characteristic: target gene


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: AATGATACGGCGACCACCGAGATCTACAC

characteristic: adapters

characteristic: collection date

text: Patient 3 blind brush

characteristic: description

text: Human

characteristic: environment (biome)

text: Lung

characteristic: environment (feature)

text: Mucus

characteristic: environment (material)

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: 51.5074� N

characteristic: geographic location (latitude)

text: 0.1278� W

characteristic: geographic location (longitude)

characteristic: host age

text: Child

text: Patient 3 blind brush

text: human-associated

text: metagenome

text: Cuthbertson et al 2017

text: PBB

characteristic: lung/pulmory disorder

text: CTCTCTACGTAAGGAG

text: Cuthbertson et al 2017

text: MPBio FastDNATM spin kit for soil

text: human lung metagenome

characteristic: organism

text: 520F, 802R

text: Lung brush

text: human lung metagenome

text: Illumina Miseq

text: 16S rRNA gene

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: AATGATACGGCGACCACCGAGATCTACAC

text: Patient 12 blind brush 1

text: Human

text: Lung

text: Mucus

text: United Kingdom

text: 51.5074� N

text: 0.1278� W

text: Child

characteristic: host family relationship

text: Patient 12 blind brush 1

characteristic: host subject id

text: human-associated

characteristic: human associated environmental package

text: metagenome

characteristic: investigation type

text: Cuthbertson et al 2017

characteristic: library construction method

text: GCTACGCTTATCCTCT

characteristic: multiplex identifiers

text: Cuthbertson et al 2017

characteristic: nucleic acid amplification

text: MPBio FastDNATM spin kit for soil

characteristic: nucleic acid extraction

text: human lung metagenome

text: 520F, 802R

characteristic: pcr primers

characteristic: project name

text: Lung brush

characteristic: sample collection device or method

characteristic: sample storage temperature

characteristic: sample volume or weight for DNA extraction

text: human lung metagenome

text: Illumina Miseq

characteristic: sequencing method

text: 16S rRNA gene

characteristic: target gene


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: AATGATACGGCGACCACCGAGATCTACAC

characteristic: adapters

characteristic: collection date

text: Patient 32 blind brush

characteristic: description

text: Human

characteristic: environment (biome)

text: Lung

characteristic: environment (feature)

text: Mucus

characteristic: environment (material)

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: 51.5074� N

characteristic: geographic location (latitude)

text: 0.1278� W

characteristic: geographic location (longitude)

characteristic: host age

text: Child

text: Patient 32 blind brush

text: human-associated

text: metagenome

text: Cuthbertson et al 2017

text: PBB

characteristic: lung/pulmory disorder

text: GTAGAGGAAAGGAGTA

text: Cuthbertson et al 2017

text: MPBio FastDNATM spin kit for soil

text: human lung metagenome

characteristic: organism

text: 520F, 802R

text: Lung brush

text: human lung metagenome

text: Illumina Miseq

text: 16S rRNA gene

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: AATGATACGGCGACCACCGAGATCTACAC

text: Mother of patient 34 nose

text: Human

text: Lung

text: Mucus

text: United Kingdom

text: 51.5074� N

text: 0.1278� W

text: Mother

characteristic: host family relationship

text: Mother of patient 34 nose

characteristic: host subject id

text: human-associated

characteristic: human associated environmental package

text: metagenome

characteristic: investigation type

text: Cuthbertson et al 2017

characteristic: library construction method

text: TAGGCATGAGAGTAGA

characteristic: multiplex identifiers

text: Cuthbertson et al 2017

characteristic: nucleic acid amplification

text: MPBio FastDNATM spin kit for soil

characteristic: nucleic acid extraction

text: human lung metagenome

text: 520F, 802R

characteristic: pcr primers

characteristic: project name

text: Lung brush

characteristic: sample collection device or method

characteristic: sample storage temperature

characteristic: sample volume or weight for DNA extraction

text: human lung metagenome

text: Illumina Miseq

characteristic: sequencing method

text: 16S rRNA gene

characteristic: target gene


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: AATGATACGGCGACCACCGAGATCTACAC

characteristic: adapters

characteristic: collection date

text: Patient 14 blind brush

characteristic: description

text: Human

characteristic: environment (biome)

text: Lung

characteristic: environment (feature)

text: Mucus

characteristic: environment (material)

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: 51.5074� N

characteristic: geographic location (latitude)

text: 0.1278� W

characteristic: geographic location (longitude)

characteristic: host age

text: Child

text: Patient 14 blind brush

text: human-associated

text: metagenome

text: Cuthbertson et al 2017

text: PBB

characteristic: lung/pulmory disorder

text: TAGGCATGCTAAGCCT

text: Cuthbertson et al 2017

text: MPBio FastDNATM spin kit for soil

text: human lung metagenome

characteristic: organism

text: 520F, 802R

text: Lung brush

text: human lung metagenome

text: Illumina Miseq

text: 16S rRNA gene

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: AATGATACGGCGACCACCGAGATCTACAC

text: Patient 39 blind brush

text: Human

text: Lung

text: Mucus

text: United Kingdom

text: 51.5074� N

text: 0.1278� W

text: Child

characteristic: host family relationship

text: Patient 39 blind brush

characteristic: host subject id

text: human-associated

characteristic: human associated environmental package

text: metagenome

characteristic: investigation type

text: Cuthbertson et al 2017

characteristic: library construction method

text: PBB

text: TAGGCATGAAGGAGTA

characteristic: multiplex identifiers

text: Cuthbertson et al 2017

characteristic: nucleic acid amplification

text: MPBio FastDNATM spin kit for soil

characteristic: nucleic acid extraction

text: human lung metagenome

text: 520F, 802R

characteristic: pcr primers

characteristic: project name

text: Lung brush

characteristic: sample collection device or method

characteristic: sample storage temperature

characteristic: sample volume or weight for DNA extraction

text: human lung metagenome

text: Illumina Miseq

characteristic: sequencing method

text: 16S rRNA gene

characteristic: target gene


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: AATGATACGGCGACCACCGAGATCTACAC

characteristic: adapters

characteristic: collection date

text: Patient 36 non blind brush

characteristic: description

text: Human

characteristic: environment (biome)

text: Lung

characteristic: environment (feature)

text: Mucus

characteristic: environment (material)

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: 51.5074� N

characteristic: geographic location (latitude)

text: 0.1278� W

characteristic: geographic location (longitude)

characteristic: host age

text: Child

text: Patient 36 non blind brush

text: human-associated

text: metagenome

text: Cuthbertson et al 2017

text: PBB

characteristic: lung/pulmory disorder

text: CGTACTAGGTAAGGAG

text: Cuthbertson et al 2017

text: MPBio FastDNATM spin kit for soil

text: human lung metagenome

characteristic: organism

text: 520F, 802R

text: Lung brush

text: human lung metagenome

text: Illumina Miseq

text: 16S rRNA gene

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: AATGATACGGCGACCACCGAGATCTACAC

text: Patient 28 blind brush

text: Human

text: Lung

text: Mucus

text: United Kingdom

text: 51.5074� N

text: 0.1278� W

text: Child

characteristic: host family relationship

text: Patient 28 blind brush

characteristic: host subject id

text: human-associated

characteristic: human associated environmental package

text: metagenome

characteristic: investigation type

text: Cuthbertson et al 2017

characteristic: library construction method

text: PBB

text: CAGAGAGGACTGCATA

characteristic: multiplex identifiers

text: Cuthbertson et al 2017

characteristic: nucleic acid amplification

text: MPBio FastDNATM spin kit for soil

characteristic: nucleic acid extraction

text: human lung metagenome

text: 520F, 802R

characteristic: pcr primers

characteristic: project name

text: Lung brush

characteristic: sample collection device or method

characteristic: sample storage temperature

characteristic: sample volume or weight for DNA extraction

text: human lung metagenome

text: Illumina Miseq

characteristic: sequencing method

text: 16S rRNA gene

characteristic: target gene


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

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text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: AATGATACGGCGACCACCGAGATCTACAC

characteristic: adapters

characteristic: collection date

text: Patient 21 blind brush

characteristic: description

text: Human

characteristic: environment (biome)

text: Lung

characteristic: environment (feature)

text: Mucus

characteristic: environment (material)

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: 51.5074� N

characteristic: geographic location (latitude)

text: 0.1278� W

characteristic: geographic location (longitude)

characteristic: host age

text: Child

text: Patient 21 blind brush

text: human-associated

text: metagenome

text: Cuthbertson et al 2017

text: PBB

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text: AGGCAGAAGTAAGGAG

text: Cuthbertson et al 2017

text: MPBio FastDNATM spin kit for soil

text: human lung metagenome

characteristic: organism

text: 520F, 802R

text: Lung brush

text: human lung metagenome

text: Illumina Miseq

text: 16S rRNA gene

characteristic: title


characteristic: ENA-CHECKLIST

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characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: AATGATACGGCGACCACCGAGATCTACAC

text: Patient 15 blind brush 1

text: Human

text: Lung

text: Mucus

text: United Kingdom

text: 51.5074� N

text: 0.1278� W

text: Child

characteristic: host family relationship

text: Patient 15 blind brush 1

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characteristic: ENA-CHECKLIST

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text: Patient 11 blind brush

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characteristic: environment (biome)

text: Lung

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text: Mucus

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characteristic: geographic location (country and/or sea)

text: 51.5074� N

characteristic: geographic location (latitude)

text: 0.1278� W

characteristic: geographic location (longitude)

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text: 520F, 802R

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characteristic: ENA-CHECKLIST

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characteristic: INSDC center name

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text: public

text: AATGATACGGCGACCACCGAGATCTACAC

text: Patient 26 non blind brush

text: Human

text: Lung

text: Mucus

text: United Kingdom

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text: 0.1278� W

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characteristic: host family relationship

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text: Patient 36 blind brush

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text: Lung

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text: Mucus

characteristic: environment (material)

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: 51.5074� N

characteristic: geographic location (latitude)

text: 0.1278� W

characteristic: geographic location (longitude)

characteristic: host age

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text: public

text: AATGATACGGCGACCACCGAGATCTACAC

text: Patient 27 blind brush 2

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text: Lung

text: Mucus

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text: 0.1278� W

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characteristic: host family relationship

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text: Patient 6 blind brush

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characteristic: environment (biome)

text: Lung

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text: Mucus

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text: United Kingdom

characteristic: geographic location (country and/or sea)

text: 51.5074� N

characteristic: geographic location (latitude)

text: 0.1278� W

characteristic: geographic location (longitude)

characteristic: host age

text: Child

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text: Mucus

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characteristic: ENA-CHECKLIST

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text: Lung

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text: Mucus

characteristic: environment (material)

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: 51.5074� N

characteristic: geographic location (latitude)

text: 0.1278� W

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text: Mucus

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text: Mucus

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characteristic: geographic location (country and/or sea)

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characteristic: geographic location (latitude)

text: 0.1278� W

characteristic: geographic location (longitude)

characteristic: host age

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text: Patient 20 blind brush 2

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text: Lung

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text: Mucus

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characteristic: geographic location (country and/or sea)

text: 51.5074� N

characteristic: geographic location (latitude)

text: 0.1278� W

characteristic: geographic location (longitude)

characteristic: host age

text: Child

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characteristic: INSDC center name

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text: Mother of patient 29 throat

characteristic: description

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characteristic: environment (biome)

text: Lung

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text: Mucus

characteristic: environment (material)

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characteristic: geographic location (country and/or sea)

text: 51.5074� N

characteristic: geographic location (latitude)

text: 0.1278� W

characteristic: geographic location (longitude)

characteristic: host age

text: Mother

text: Mother of patient 29 throat

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text: metagenome

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text: TAAGGCGAACTGCATA

text: Cuthbertson et al 2017

text: MPBio FastDNATM spin kit for soil

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characteristic: ENA-CHECKLIST

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characteristic: host family relationship

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text: United Kingdom

characteristic: geographic location (country and/or sea)

text: 51.5074� N

characteristic: geographic location (latitude)

text: 0.1278� W

characteristic: geographic location (longitude)

characteristic: host age

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text: Mother of patient 24 throat

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text: GGACTCCTCTAAGCCT

text: Cuthbertson et al 2017

text: MPBio FastDNATM spin kit for soil

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characteristic: host subject id

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characteristic: INSDC status

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characteristic: adapters

characteristic: collection date

text: Patient 7 non blind brush

characteristic: description

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characteristic: environment (biome)

text: Lung

characteristic: environment (feature)

text: Mucus

characteristic: environment (material)

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: 51.5074� N

characteristic: geographic location (latitude)

text: 0.1278� W

characteristic: geographic location (longitude)

characteristic: host age

text: Child

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text: human-associated

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text: PBB

characteristic: lung/pulmory disorder

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text: MPBio FastDNATM spin kit for soil

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characteristic: adapters

characteristic: collection date

text: Patient 33 non blind brush

characteristic: description

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characteristic: environment (biome)

text: Lung

characteristic: environment (feature)

text: Mucus

characteristic: environment (material)

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: 51.5074� N

characteristic: geographic location (latitude)

text: 0.1278� W

characteristic: geographic location (longitude)

characteristic: host age

text: Child

text: Patient 33 non blind brush

text: human-associated

text: metagenome

text: Cuthbertson et al 2017

text: PBB

characteristic: lung/pulmory disorder

text: GCTACGCTCTCTCTAT

text: Cuthbertson et al 2017

text: MPBio FastDNATM spin kit for soil

text: human lung metagenome

characteristic: organism

text: 520F, 802R

text: Lung brush

text: human lung metagenome

text: Illumina Miseq

text: 16S rRNA gene

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: AATGATACGGCGACCACCGAGATCTACAC

text: Patient 2 non blind brush

text: Human

text: Lung

text: Mucus

text: United Kingdom

text: 51.5074� N

text: 0.1278� W

text: Child

characteristic: host family relationship

text: Patient 2 non blind brush

characteristic: host subject id

text: human-associated

characteristic: human associated environmental package

text: metagenome

characteristic: investigation type

text: Cuthbertson et al 2017

characteristic: library construction method

text: PBB

text: CGAGGCTGAGAGTAGA

characteristic: multiplex identifiers

text: Cuthbertson et al 2017

characteristic: nucleic acid amplification

text: MPBio FastDNATM spin kit for soil

characteristic: nucleic acid extraction

text: human lung metagenome

text: 520F, 802R

characteristic: pcr primers

characteristic: project name

text: Lung brush

characteristic: sample collection device or method

characteristic: sample storage temperature

characteristic: sample volume or weight for DNA extraction

text: human lung metagenome

text: Illumina Miseq

characteristic: sequencing method

text: 16S rRNA gene

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characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

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text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: AATGATACGGCGACCACCGAGATCTACAC

characteristic: adapters

characteristic: collection date

text: Mother of patient 3 throat

characteristic: description

text: Human

characteristic: environment (biome)

text: Lung

characteristic: environment (feature)

text: Mucus

characteristic: environment (material)

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: 51.5074� N

characteristic: geographic location (latitude)

text: 0.1278� W

characteristic: geographic location (longitude)

characteristic: host age

text: Mother

text: Mother of patient 3 throat

text: human-associated

text: metagenome

text: Cuthbertson et al 2017

text: AGGCAGAAACTGCATA

text: Cuthbertson et al 2017

text: MPBio FastDNATM spin kit for soil

text: human lung metagenome

characteristic: organism

text: 520F, 802R

text: Lung brush

text: human lung metagenome

text: Illumina Miseq

text: 16S rRNA gene

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: AATGATACGGCGACCACCGAGATCTACAC

text: Mother of patient 3 nose

text: Human

text: Lung

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text: United Kingdom

text: 51.5074� N

text: 0.1278� W

text: Mother

characteristic: host family relationship

text: Mother of patient 3 nose

characteristic: host subject id

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characteristic: human associated environmental package

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characteristic: library construction method

text: AAGAGGCATATCCTCT

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text: Cuthbertson et al 2017

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text: MPBio FastDNATM spin kit for soil

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characteristic: sample collection device or method

characteristic: sample storage temperature

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text: human lung metagenome

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characteristic: sequencing method

text: 16S rRNA gene

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characteristic: ENA-CHECKLIST

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characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

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text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: AATGATACGGCGACCACCGAGATCTACAC

characteristic: adapters

characteristic: collection date

text: Mother of patient 37 throat

characteristic: description

text: Human

characteristic: environment (biome)

text: Lung

characteristic: environment (feature)

text: Mucus

characteristic: environment (material)

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: 51.5074� N

characteristic: geographic location (latitude)

text: 0.1278� W

characteristic: geographic location (longitude)

characteristic: host age

text: Mother

text: Mother of patient 37 throat

text: human-associated

text: metagenome

text: Cuthbertson et al 2017

text: TCCTGAGCAAGGAGTA

text: Cuthbertson et al 2017

text: MPBio FastDNATM spin kit for soil

text: human lung metagenome

characteristic: organism

text: 520F, 802R

text: Lung brush

text: human lung metagenome

text: Illumina Miseq

text: 16S rRNA gene

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: AATGATACGGCGACCACCGAGATCTACAC

text: Patient 45 blind brush 1

text: Human

text: Lung

text: Mucus

text: United Kingdom

text: 51.5074� N

text: 0.1278� W

text: Child

characteristic: host family relationship

text: Patient 45 blind brush 1

characteristic: host subject id

text: human-associated

characteristic: human associated environmental package

text: metagenome

characteristic: investigation type

text: Cuthbertson et al 2017

characteristic: library construction method

text: CGTACTAGCTAAGCCT

characteristic: multiplex identifiers

text: Cuthbertson et al 2017

characteristic: nucleic acid amplification

text: MPBio FastDNATM spin kit for soil

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text: human lung metagenome

text: 520F, 802R

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characteristic: project name

text: Lung brush

characteristic: sample collection device or method

characteristic: sample storage temperature

characteristic: sample volume or weight for DNA extraction

text: human lung metagenome

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characteristic: sequencing method

text: 16S rRNA gene

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characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

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text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: AATGATACGGCGACCACCGAGATCTACAC

characteristic: adapters

characteristic: collection date

text: Mother of patient 14 throat

characteristic: description

text: Human

characteristic: environment (biome)

text: Lung

characteristic: environment (feature)

text: Mucus

characteristic: environment (material)

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: 51.5074� N

characteristic: geographic location (latitude)

text: 0.1278� W

characteristic: geographic location (longitude)

characteristic: host age

text: Mother

text: Mother of patient 14 throat

text: human-associated

text: metagenome

text: Cuthbertson et al 2017

text: CTCTCTACCTAAGCCT

text: Cuthbertson et al 2017

text: MPBio FastDNATM spin kit for soil

text: human lung metagenome

characteristic: organism

text: 520F, 802R

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text: human lung metagenome

text: Illumina Miseq

text: 16S rRNA gene

characteristic: title


characteristic: ENA-CHECKLIST

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characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

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text: public

text: AATGATACGGCGACCACCGAGATCTACAC

text: Patient 31 blind brush 1

text: Human

text: Lung

text: Mucus

text: United Kingdom

text: 51.5074� N

text: 0.1278� W

text: Child

characteristic: host family relationship

text: Patient 31 blind brush 1

characteristic: host subject id

text: human-associated

characteristic: human associated environmental package

text: metagenome

characteristic: investigation type

text: Cuthbertson et al 2017

characteristic: library construction method

text: TAGGCATGTATCCTCT

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text: Cuthbertson et al 2017

characteristic: nucleic acid amplification

text: MPBio FastDNATM spin kit for soil

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text: human lung metagenome

text: 520F, 802R

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characteristic: project name

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text: human lung metagenome

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text: 16S rRNA gene

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characteristic: ENA-CHECKLIST

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characteristic: External Id

characteristic: INSDC center name

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text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: AATGATACGGCGACCACCGAGATCTACAC

characteristic: adapters

characteristic: collection date

text: Patient 4 blind brush

characteristic: description

text: Human

characteristic: environment (biome)

text: Lung

characteristic: environment (feature)

text: Mucus

characteristic: environment (material)

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: 51.5074� N

characteristic: geographic location (latitude)

text: 0.1278� W

characteristic: geographic location (longitude)

characteristic: host age

text: Child

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text: human-associated

text: metagenome

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text: PBB

characteristic: lung/pulmory disorder

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text: MPBio FastDNATM spin kit for soil

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text: Illumina Miseq

text: 16S rRNA gene

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characteristic: ENA-CHECKLIST

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characteristic: INSDC center name

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text: public

text: AATGATACGGCGACCACCGAGATCTACAC

text: Patient 3 non blind brush

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text: Mucus

text: United Kingdom

text: 51.5074� N

text: 0.1278� W

text: Child

characteristic: host family relationship

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characteristic: host subject id

text: human-associated

characteristic: human associated environmental package

text: metagenome

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text: AAGAGGCACTCTCTAT

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text: Cuthbertson et al 2017

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characteristic: sequencing method

text: 16S rRNA gene

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characteristic: ENA-CHECKLIST

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characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

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text: public

characteristic: INSDC status

characteristic: SRA accession

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characteristic: adapters

characteristic: collection date

text: Mother of patient 18 throat

characteristic: description

text: Human

characteristic: environment (biome)

text: Lung

characteristic: environment (feature)

text: Mucus

characteristic: environment (material)

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: 51.5074� N

characteristic: geographic location (latitude)

text: 0.1278� W

characteristic: geographic location (longitude)

characteristic: host age

text: Mother

text: Mother of patient 18 throat

text: human-associated

text: metagenome

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text: CAGAGAGGCTAAGCCT

text: Cuthbertson et al 2017

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text: Illumina Miseq

text: 16S rRNA gene

characteristic: title


characteristic: ENA-CHECKLIST

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characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: AATGATACGGCGACCACCGAGATCTACAC

text: Mother of patient 37 nose

text: Human

text: Lung

text: Mucus

text: United Kingdom

text: 51.5074� N

text: 0.1278� W

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characteristic: host family relationship

text: Mother of patient 37 nose

characteristic: host subject id

text: human-associated

characteristic: human associated environmental package

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characteristic: investigation type

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characteristic: library construction method

text: TCCTGAGCCTCTCTAT

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text: Cuthbertson et al 2017

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text: MPBio FastDNATM spin kit for soil

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characteristic: sequencing method

text: 16S rRNA gene

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characteristic: ENA-CHECKLIST

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text: Mother of patient 5 throat

characteristic: description

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characteristic: environment (biome)

text: Lung

characteristic: environment (feature)

text: Mucus

characteristic: environment (material)

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: 51.5074� N

characteristic: geographic location (latitude)

text: 0.1278� W

characteristic: geographic location (longitude)

characteristic: host age

text: Mother

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text: GCTACGCTCTAAGCCT

text: Cuthbertson et al 2017

text: MPBio FastDNATM spin kit for soil

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characteristic: ENA-CHECKLIST

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characteristic: INSDC center name

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text: public

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text: Human

text: Lung

text: Mucus

text: United Kingdom

text: 51.5074� N

text: 0.1278� W

text: Child

characteristic: host family relationship

text: Patient 39 non blind brush

characteristic: host subject id

text: human-associated

characteristic: human associated environmental package

text: metagenome

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characteristic: library construction method

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characteristic: lung/pulmory disorder

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characteristic: sequencing method

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characteristic: ENA-CHECKLIST

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characteristic: INSDC center name

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text: public

characteristic: INSDC status

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characteristic: Submitter Id

text: AATGATACGGCGACCACCGAGATCTACAC

characteristic: adapters

characteristic: collection date

text: Patient 8 non blind brush

characteristic: description

text: Human

characteristic: environment (biome)

text: Lung

characteristic: environment (feature)

text: Mucus

characteristic: environment (material)

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: 51.5074� N

characteristic: geographic location (latitude)

text: 0.1278� W

characteristic: geographic location (longitude)

characteristic: host age

text: Child

text: Patient 8 non blind brush

text: human-associated

text: metagenome

text: Cuthbertson et al 2017

text: PBB

text: TCCTGAGCTATCCTCT

text: Cuthbertson et al 2017

text: MPBio FastDNATM spin kit for soil

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characteristic: ENA-CHECKLIST

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characteristic: INSDC center name

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text: public

text: AATGATACGGCGACCACCGAGATCTACAC

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text: Lung

text: Mucus

text: United Kingdom

text: 51.5074� N

text: 0.1278� W

text: Child

characteristic: host family relationship

text: Patient 21 non blind brush

characteristic: host subject id

text: human-associated

characteristic: human associated environmental package

text: metagenome

characteristic: investigation type

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text: Cuthbertson et al 2017

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characteristic: collection date

text: Patient 30 non blind brush

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text: Lung

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text: Mucus

characteristic: environment (material)

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: 51.5074� N

characteristic: geographic location (latitude)

text: 0.1278� W

characteristic: geographic location (longitude)

characteristic: host age

text: Child

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text: human-associated

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text: PBB

text: AGGCAGAACTAAGCCT

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text: public

text: AATGATACGGCGACCACCGAGATCTACAC

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text: 0.1278� W

text: Child

characteristic: host family relationship

text: Patient 5 blind brush

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text: Cuthbertson et al 2017

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characteristic: INSDC center name

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characteristic: adapters

characteristic: collection date

text: Mother of patient 5 nose

characteristic: description

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characteristic: environment (biome)

text: Lung

characteristic: environment (feature)

text: Mucus

characteristic: environment (material)

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: 51.5074� N

characteristic: geographic location (latitude)

text: 0.1278� W

characteristic: geographic location (longitude)

characteristic: host age

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text: Mother of patient 5 nose

text: human-associated

text: metagenome

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text: GCTACGCTAGAGTAGA

text: Cuthbertson et al 2017

text: MPBio FastDNATM spin kit for soil

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characteristic: organism

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text: Illumina Miseq

text: 16S rRNA gene

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characteristic: INSDC center name

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text: public

text: AATGATACGGCGACCACCGAGATCTACAC

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text: Human

text: Lung

text: Mucus

text: United Kingdom

text: 51.5074� N

text: 0.1278� W

text: Mother

characteristic: host family relationship

text: Mother of patient 39 nose

characteristic: host subject id

text: human-associated

characteristic: human associated environmental package

text: metagenome

characteristic: investigation type

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characteristic: library construction method

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text: Cuthbertson et al 2017

characteristic: nucleic acid amplification

text: MPBio FastDNATM spin kit for soil

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characteristic: External Id

characteristic: INSDC center name

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text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: AATGATACGGCGACCACCGAGATCTACAC

characteristic: adapters

characteristic: collection date

text: Patient 37 non blind brush

characteristic: description

text: Human

characteristic: environment (biome)

text: Lung

characteristic: environment (feature)

text: Mucus

characteristic: environment (material)

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: 51.5074� N

characteristic: geographic location (latitude)

text: 0.1278� W

characteristic: geographic location (longitude)

characteristic: host age

text: Child

text: Patient 37 non blind brush

text: human-associated

text: metagenome

text: Cuthbertson et al 2017

text: PBB

characteristic: lung/pulmory disorder

text: GGACTCCTAAGGAGTA

text: Cuthbertson et al 2017

text: MPBio FastDNATM spin kit for soil

text: human lung metagenome

characteristic: organism

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text: Illumina Miseq

text: 16S rRNA gene

characteristic: title


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characteristic: INSDC center name

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text: AATGATACGGCGACCACCGAGATCTACAC

text: Mother of patient 36 nose

text: Human

text: Lung

text: Mucus

text: United Kingdom

text: 51.5074� N

text: 0.1278� W

text: Mother

characteristic: host family relationship

text: Mother of patient 36 nose

characteristic: host subject id

text: human-associated

characteristic: human associated environmental package

text: metagenome

characteristic: investigation type

text: Cuthbertson et al 2017

characteristic: library construction method

text: CGAGGCTGACTGCATA

characteristic: multiplex identifiers

text: Cuthbertson et al 2017

characteristic: nucleic acid amplification

text: MPBio FastDNATM spin kit for soil

characteristic: nucleic acid extraction

text: human lung metagenome

text: 520F, 802R

characteristic: pcr primers

characteristic: project name

text: Lung brush

characteristic: sample collection device or method

characteristic: sample storage temperature

characteristic: sample volume or weight for DNA extraction

text: human lung metagenome

text: Illumina Miseq

characteristic: sequencing method

text: 16S rRNA gene

characteristic: target gene


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: AATGATACGGCGACCACCGAGATCTACAC

characteristic: adapters

characteristic: collection date

text: Patient 43 blind brush

characteristic: description

text: Human

characteristic: environment (biome)

text: Lung

characteristic: environment (feature)

text: Mucus

characteristic: environment (material)

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: 51.5074� N

characteristic: geographic location (latitude)

text: 0.1278� W

characteristic: geographic location (longitude)

characteristic: host age

text: Child

text: Patient 43 blind brush

text: human-associated

text: metagenome

text: Cuthbertson et al 2017

text: PBB

characteristic: lung/pulmory disorder

text: CGTACTAGACTGCATA

text: Cuthbertson et al 2017

text: MPBio FastDNATM spin kit for soil

text: human lung metagenome

characteristic: organism

text: 520F, 802R

text: Lung brush

text: human lung metagenome

text: Illumina Miseq

text: 16S rRNA gene

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: AATGATACGGCGACCACCGAGATCTACAC

text: Patient 5 non blind brush

text: Human

text: Lung

text: Mucus

text: United Kingdom

text: 51.5074� N

text: 0.1278� W

text: Child

characteristic: host family relationship

text: Patient 5 non blind brush

characteristic: host subject id

text: human-associated

characteristic: human associated environmental package

text: metagenome

characteristic: investigation type

text: Cuthbertson et al 2017

characteristic: library construction method

text: PBB

text: CAGAGAGGTATCCTCT

characteristic: multiplex identifiers

text: Cuthbertson et al 2017

characteristic: nucleic acid amplification

text: MPBio FastDNATM spin kit for soil

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text: human lung metagenome

text: 520F, 802R

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characteristic: project name

text: Lung brush

characteristic: sample collection device or method

characteristic: sample storage temperature

characteristic: sample volume or weight for DNA extraction

text: human lung metagenome

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characteristic: sequencing method

text: 16S rRNA gene

characteristic: target gene


characteristic: ENA-CHECKLIST

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characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

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text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: AATGATACGGCGACCACCGAGATCTACAC

characteristic: adapters

characteristic: collection date

text: Mother of patient 26 throat

characteristic: description

text: Human

characteristic: environment (biome)

text: Lung

characteristic: environment (feature)

text: Mucus

characteristic: environment (material)

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: 51.5074� N

characteristic: geographic location (latitude)

text: 0.1278� W

characteristic: geographic location (longitude)

characteristic: host age

text: Mother

text: Mother of patient 26 throat

text: human-associated

text: metagenome

text: Cuthbertson et al 2017

text: CGAGGCTGCTAAGCCT

text: Cuthbertson et al 2017

text: MPBio FastDNATM spin kit for soil

text: human lung metagenome

characteristic: organism

text: 520F, 802R

text: Lung brush

text: human lung metagenome

text: Illumina Miseq

text: 16S rRNA gene

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC last update

text: public

text: AATGATACGGCGACCACCGAGATCTACAC

text: Patient 14 non blind brush

text: Human

text: Lung

text: Mucus

text: United Kingdom

text: 51.5074� N

text: 0.1278� W

text: Child

characteristic: host family relationship

text: Patient 14 non blind brush

characteristic: host subject id

text: human-associated

characteristic: human associated environmental package

text: metagenome

characteristic: investigation type

text: Cuthbertson et al 2017

characteristic: library construction method

text: PBB

characteristic: lung/pulmory disorder

text: TCCTGAGCCTAAGCCT

characteristic: multiplex identifiers

text: Cuthbertson et al 2017

characteristic: nucleic acid amplification

text: MPBio FastDNATM spin kit for soil

characteristic: nucleic acid extraction

text: human lung metagenome

text: 520F, 802R

characteristic: pcr primers

characteristic: project name

text: Lung brush

characteristic: sample collection device or method

characteristic: sample storage temperature

characteristic: sample volume or weight for DNA extraction

text: human lung metagenome

text: Illumina Miseq

characteristic: sequencing method

text: 16S rRNA gene

characteristic: target gene


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: AATGATACGGCGACCACCGAGATCTACAC

characteristic: adapters

characteristic: collection date

text: Patient 23 blind brush 1

characteristic: description

text: Human

characteristic: environment (biome)

text: Lung

characteristic: environment (feature)

text: Mucus

characteristic: environment (material)

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: 51.5074� N

characteristic: geographic location (latitude)

text: 0.1278� W

characteristic: geographic location (longitude)

characteristic: host age

text: Child

text: Patient 23 blind brush 1

text: human-associated

text: metagenome

text: Cuthbertson et al 2017

text: AGGCAGAAAGAGTAGA

text: Cuthbertson et al 2017

text: MPBio FastDNATM spin kit for soil

text: human lung metagenome

characteristic: organism

text: 520F, 802R

text: Lung brush

text: human lung metagenome

text: Illumina Miseq

text: 16S rRNA gene

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: AATGATACGGCGACCACCGAGATCTACAC

text: Patient 17 blind brush

text: Human

text: Lung

text: Mucus

text: United Kingdom

text: 51.5074� N

text: 0.1278� W

text: Child

characteristic: host family relationship

text: Patient 17 blind brush

characteristic: host subject id

text: human-associated

characteristic: human associated environmental package

text: metagenome

characteristic: investigation type

text: Cuthbertson et al 2017

characteristic: library construction method

text: PBB

characteristic: lung/pulmory disorder

text: CTCTCTACACTGCATA

characteristic: multiplex identifiers

text: Cuthbertson et al 2017

characteristic: nucleic acid amplification

text: MPBio FastDNATM spin kit for soil

characteristic: nucleic acid extraction

text: human lung metagenome

text: 520F, 802R

characteristic: pcr primers

characteristic: project name

text: Lung brush

characteristic: sample collection device or method

characteristic: sample storage temperature

characteristic: sample volume or weight for DNA extraction

text: human lung metagenome

text: Illumina Miseq

characteristic: sequencing method

text: 16S rRNA gene

characteristic: target gene


characteristic: ENA-CHECKLIST

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characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: AATGATACGGCGACCACCGAGATCTACAC

characteristic: adapters

characteristic: collection date

text: Patient 40 blind brush 2

characteristic: description

text: Human

characteristic: environment (biome)

text: Lung

characteristic: environment (feature)

text: Mucus

characteristic: environment (material)

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: 51.5074� N

characteristic: geographic location (latitude)

text: 0.1278� W

characteristic: geographic location (longitude)

characteristic: host age

text: Child

text: Patient 40 blind brush 2

text: human-associated

text: metagenome

text: Cuthbertson et al 2017

text: GTAGAGGACTCTCTAT

text: Cuthbertson et al 2017

text: MPBio FastDNATM spin kit for soil

text: human lung metagenome

characteristic: organism

text: 520F, 802R

text: Lung brush

text: human lung metagenome

text: Illumina Miseq

text: 16S rRNA gene

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: AATGATACGGCGACCACCGAGATCTACAC

text: Patient 28 non blind brush

text: Human

text: Lung

text: Mucus

text: United Kingdom

text: 51.5074� N

text: 0.1278� W

text: Child

characteristic: host family relationship

text: Patient 28 non blind brush

characteristic: host subject id

text: human-associated

characteristic: human associated environmental package

text: metagenome

characteristic: investigation type

text: Cuthbertson et al 2017

characteristic: library construction method

text: PBB

characteristic: lung/pulmory disorder

text: TCCTGAGCACTGCATA

characteristic: multiplex identifiers

text: Cuthbertson et al 2017

characteristic: nucleic acid amplification

text: MPBio FastDNATM spin kit for soil

characteristic: nucleic acid extraction

text: human lung metagenome

text: 520F, 802R

characteristic: pcr primers

characteristic: project name

text: Lung brush

characteristic: sample collection device or method

characteristic: sample storage temperature

characteristic: sample volume or weight for DNA extraction

text: human lung metagenome

text: Illumina Miseq

characteristic: sequencing method

text: 16S rRNA gene

characteristic: target gene


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: AATGATACGGCGACCACCGAGATCTACAC

characteristic: adapters

characteristic: collection date

text: Patient 18 blind brush 1

characteristic: description

text: Human

characteristic: environment (biome)

text: Lung

characteristic: environment (feature)

text: Mucus

characteristic: environment (material)

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: 51.5074� N

characteristic: geographic location (latitude)

text: 0.1278� W

characteristic: geographic location (longitude)

characteristic: host age

text: Child

text: Patient 18 blind brush 1

text: human-associated

text: metagenome

text: Cuthbertson et al 2017

text: AAGAGGCAACTGCATA

text: Cuthbertson et al 2017

text: MPBio FastDNATM spin kit for soil

text: human lung metagenome

characteristic: organism

text: 520F, 802R

text: Lung brush

text: human lung metagenome

text: Illumina Miseq

text: 16S rRNA gene

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: AATGATACGGCGACCACCGAGATCTACAC

text: Patient 30 blind brush

text: Human

text: Lung

text: Mucus

text: United Kingdom

text: 51.5074� N

text: 0.1278� W

text: Child

characteristic: host family relationship

text: Patient 30 blind brush

characteristic: host subject id

text: human-associated

characteristic: human associated environmental package

text: metagenome

characteristic: investigation type

text: Cuthbertson et al 2017

characteristic: library construction method

text: PBB

characteristic: lung/pulmory disorder

text: TAAGGCGAAAGGAGTA

characteristic: multiplex identifiers

text: Cuthbertson et al 2017

characteristic: nucleic acid amplification

text: MPBio FastDNATM spin kit for soil

characteristic: nucleic acid extraction

text: human lung metagenome

text: 520F, 802R

characteristic: pcr primers

characteristic: project name

text: Lung brush

characteristic: sample collection device or method

characteristic: sample storage temperature

characteristic: sample volume or weight for DNA extraction

text: human lung metagenome

text: Illumina Miseq

characteristic: sequencing method

text: 16S rRNA gene

characteristic: target gene


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: AATGATACGGCGACCACCGAGATCTACAC

characteristic: adapters

characteristic: collection date

text: Patient 35 blind brush 2

characteristic: description

text: Human

characteristic: environment (biome)

text: Lung

characteristic: environment (feature)

text: Mucus

characteristic: environment (material)

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: 51.5074� N

characteristic: geographic location (latitude)

text: 0.1278� W

characteristic: geographic location (longitude)

characteristic: host age

text: Child

text: Patient 35 blind brush 2

text: human-associated

text: metagenome

text: Cuthbertson et al 2017

text: CTCTCTACTATCCTCT

text: Cuthbertson et al 2017

text: MPBio FastDNATM spin kit for soil

text: human lung metagenome

characteristic: organism

text: 520F, 802R

text: Lung brush

text: human lung metagenome

text: Illumina Miseq

text: 16S rRNA gene

characteristic: title