characteristic: ENA-CHECKLIST

text: UCSDMI

text: public

text: qiita_sid_945:945.P9.G3.lane3.NoIndex.L003

text: Southwest

text: TRUE

characteristic: elevation

text: large lake biome

characteristic: env biome

text: freshwater lake

characteristic: env feature

text: water

characteristic: env package

text: fresh water

text: TRUE

characteristic: extracted DNA avail now

text: Germany:Fuchskuhle Lake

characteristic: geo loc name

characteristic: host subject id

text: Fuchskuhle

characteristic: lake

characteristic: latitude

characteristic: longitude

text: freshwater metagenome

characteristic: oxygen

characteristic: ph

text: Missing: Not provided

text: ANL

text: TRUE

text: TRUE

characteristic: public

text: Missing: Not provided

text: fresh water

characteristic: sample type

text: freshwater metagenome

characteristic: secchi depth

characteristic: temp

text: Routine samples of German Lakes


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: qiita_sid_945:945.P9.G4.lane3.NoIndex.L003

characteristic: Submitter Id

characteristic: anonymized name

text: Southwest

characteristic: basin

characteristic: conductivity

characteristic: depth

text: freshwater metagenome, Lake Grosse Fuchskuhle

characteristic: description

text: TRUE

text: large lake biome

text: freshwater lake

text: water

text: fresh water

text: TRUE

text: Germany:Fuchskuhle Lake

text: Fuchskuhle

text: freshwater metagenome

characteristic: organism

text: Missing: Not provided

text: ANL

text: TRUE

text: TRUE

text: Missing: Not provided

text: fresh water

text: freshwater metagenome

text: Routine samples of German Lakes

characteristic: title

type: has member


characteristic: ENA-CHECKLIST

text: UCSDMI

text: public

text: qiita_sid_945:945.P9.G5.lane3.NoIndex.L003

text: Southwest

text: TRUE

characteristic: elevation

text: large lake biome

characteristic: env biome

text: freshwater lake

characteristic: env feature

text: water

characteristic: env package

text: fresh water

text: TRUE

characteristic: extracted DNA avail now

text: Germany:Fuchskuhle Lake

characteristic: geo loc name

characteristic: host subject id

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characteristic: lake

characteristic: latitude

characteristic: longitude

text: freshwater metagenome

characteristic: oxygen

characteristic: ph

text: Missing: Not provided

text: ANL

text: TRUE

text: TRUE

characteristic: public

text: Missing: Not provided

text: fresh water

characteristic: sample type

text: freshwater metagenome

characteristic: secchi depth

characteristic: temp

text: Routine samples of German Lakes


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: qiita_sid_945:945.P9.G6.lane3.NoIndex.L003

characteristic: Submitter Id

characteristic: anonymized name

text: Southwest

characteristic: basin

characteristic: conductivity

characteristic: depth

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text: TRUE

text: large lake biome

text: freshwater lake

text: water

text: fresh water

text: TRUE

text: Germany:Fuchskuhle Lake

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text: freshwater metagenome

characteristic: organism

text: Missing: Not provided

text: ANL

text: TRUE

text: TRUE

text: Missing: Not provided

text: fresh water

text: freshwater metagenome

characteristic: title

text: Routine samples of German Lakes


characteristic: ENA-CHECKLIST

text: UCSDMI

text: public

text: qiita_sid_945:945.P9.G7.lane3.NoIndex.L003

text: deepest point

text: freshwater metagenome, Lake Melzer

text: TRUE

characteristic: elevation

text: large lake biome

characteristic: env biome

text: freshwater lake

characteristic: env feature

text: water

characteristic: env package

text: fresh water

text: TRUE

characteristic: extracted DNA avail now

text: Germany:Lake Melzer Lake

characteristic: geo loc name

characteristic: host subject id

text: Lake Melzer

characteristic: lake

characteristic: latitude

characteristic: longitude

text: freshwater metagenome

characteristic: oxygen

characteristic: ph

text: Missing: Not provided

text: ANL

text: TRUE

text: TRUE

characteristic: public

text: Missing: Not provided

text: fresh water

characteristic: sample type

text: freshwater metagenome

characteristic: secchi depth

characteristic: temp

text: Routine samples of German Lakes


characteristic: ENA-CHECKLIST

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text: qiita_sid_945:945.P9.G8.lane3.NoIndex.L003

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characteristic: anonymized name

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characteristic: description

text: TRUE

text: large lake biome

text: freshwater lake

text: water

text: fresh water

text: TRUE

text: Germany:Lake Melzer Lake

text: Lake Melzer

text: freshwater metagenome

characteristic: organism

text: Missing: Not provided

text: ANL

text: TRUE

text: TRUE

text: Missing: Not provided

text: fresh water

text: freshwater metagenome

text: Routine samples of German Lakes

characteristic: title


characteristic: ENA-CHECKLIST

text: UCSDMI

text: public

text: qiita_sid_945:945.P9.G9.lane3.NoIndex.L003

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text: TRUE

characteristic: elevation

text: large lake biome

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text: freshwater lake

characteristic: env feature

text: water

characteristic: env package

text: fresh water

text: TRUE

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characteristic: geo loc name

characteristic: host subject id

text: Lake Melzer

characteristic: lake

characteristic: latitude

characteristic: longitude

text: freshwater metagenome

characteristic: oxygen

characteristic: ph

text: Missing: Not provided

text: ANL

text: TRUE

text: TRUE

characteristic: public

text: Missing: Not provided

text: fresh water

characteristic: sample type

text: freshwater metagenome

characteristic: secchi depth

characteristic: temp

text: Routine samples of German Lakes


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text: public

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characteristic: Submitter Id

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characteristic: basin

characteristic: conductivity

characteristic: depth

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text: large lake biome

text: freshwater lake

text: water

text: fresh water

text: TRUE

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characteristic: organism

text: Missing: Not provided

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text: TRUE

text: TRUE

text: Missing: Not provided

text: fresh water

text: freshwater metagenome

characteristic: title

text: Routine samples of German Lakes


characteristic: ENA-CHECKLIST

text: UCSDMI

text: public

text: qiita_sid_945:945.P9.H10.lane3.NoIndex.L003

text: deepest point

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text: TRUE

characteristic: elevation

text: large lake biome

characteristic: env biome

text: freshwater lake

characteristic: env feature

text: water

characteristic: env package

text: fresh water

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characteristic: geo loc name

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characteristic: lake

characteristic: latitude

characteristic: longitude

text: freshwater metagenome

characteristic: oxygen

characteristic: ph

text: Missing: Not provided

text: ANL

text: TRUE

text: TRUE

characteristic: public

text: Missing: Not provided

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characteristic: sample type

text: freshwater metagenome

characteristic: secchi depth

characteristic: temp

text: Routine samples of German Lakes


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text: qiita_sid_945:945.P9.H11.lane3.NoIndex.L003

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characteristic: conductivity

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characteristic: description

text: TRUE

text: large lake biome

text: freshwater lake

text: water

text: fresh water

text: TRUE

text: Germany:Lake Melzer Lake

text: Lake Melzer

text: freshwater metagenome

characteristic: organism

text: Missing: Not provided

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text: TRUE

text: TRUE

text: Missing: Not provided

text: fresh water

text: freshwater metagenome

text: Routine samples of German Lakes

characteristic: title


characteristic: ENA-CHECKLIST

text: UCSDMI

text: public

text: qiita_sid_945:945.P9.H12.lane3.NoIndex.L003

text: deepest point

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text: TRUE

characteristic: elevation

text: large lake biome

characteristic: env biome

text: freshwater lake

characteristic: env feature

text: water

characteristic: env package

text: fresh water

text: TRUE

characteristic: extracted DNA avail now

text: Germany:Lake Melzer Lake

characteristic: geo loc name

characteristic: host subject id

text: Lake Melzer

characteristic: lake

characteristic: latitude

characteristic: longitude

text: freshwater metagenome

characteristic: oxygen

characteristic: ph

text: Missing: Not provided

text: ANL

text: TRUE

text: TRUE

characteristic: public

text: Missing: Not provided

text: fresh water

characteristic: sample type

text: freshwater metagenome

characteristic: secchi depth

characteristic: temp

text: Routine samples of German Lakes


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

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text: public

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text: qiita_sid_945:945.P9.H2.lane2.NoIndex.L002

characteristic: Submitter Id

characteristic: anonymized name

text: Southwest

characteristic: basin

characteristic: conductivity

characteristic: depth

characteristic: description

text: TRUE

text: large lake biome

text: freshwater lake

text: water

text: fresh water

text: TRUE

text: Germany:Fuchskuhle Lake

text: Fuchskuhle

text: freshwater metagenome

characteristic: organism

text: Missing: Not provided

text: ANL

text: TRUE

text: TRUE

text: Missing: Not provided

text: fresh water

text: freshwater metagenome

characteristic: title

text: Routine samples of German Lakes


characteristic: ENA-CHECKLIST

text: UCSDMI

text: public

text: qiita_sid_945:945.P9.H3.lane3.NoIndex.L003

text: Southwest

text: TRUE

characteristic: elevation

text: large lake biome

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text: water

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text: TRUE

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text: Germany:Fuchskuhle Lake

characteristic: geo loc name

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text: Fuchskuhle

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characteristic: latitude

characteristic: longitude

text: freshwater metagenome

characteristic: oxygen

characteristic: ph

text: Missing: Not provided

text: ANL

text: TRUE

text: TRUE

characteristic: public

text: Missing: Not provided

text: fresh water

characteristic: sample type

text: freshwater metagenome

characteristic: secchi depth

characteristic: temp

text: Routine samples of German Lakes


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text: public

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text: qiita_sid_945:945.P9.H4.lane3.NoIndex.L003

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text: Southwest

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characteristic: conductivity

characteristic: depth

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text: TRUE

text: large lake biome

text: freshwater lake

text: water

text: fresh water

text: TRUE

text: Germany:Fuchskuhle Lake

text: Fuchskuhle

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characteristic: organism

text: Missing: Not provided

text: ANL

text: TRUE

text: TRUE

text: Missing: Not provided

text: fresh water

text: freshwater metagenome

characteristic: title

text: Routine samples of German Lakes


characteristic: ENA-CHECKLIST

text: UCSDMI

text: public

text: qiita_sid_945:945.P9.H5.lane3.NoIndex.L003

text: Southwest

text: TRUE

characteristic: elevation

text: large lake biome

characteristic: env biome

text: freshwater lake

characteristic: env feature

text: water

characteristic: env package

text: fresh water

text: TRUE

characteristic: extracted DNA avail now

text: Germany:Fuchskuhle Lake

characteristic: geo loc name

characteristic: host subject id

text: Fuchskuhle

characteristic: lake

characteristic: latitude

characteristic: longitude

text: freshwater metagenome

characteristic: oxygen

characteristic: ph

text: Missing: Not provided

text: ANL

text: TRUE

text: TRUE

characteristic: public

text: Missing: Not provided

text: fresh water

characteristic: sample type

text: freshwater metagenome

characteristic: secchi depth

characteristic: temp

text: Routine samples of German Lakes


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

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characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: qiita_sid_945:945.P9.H6.lane3.NoIndex.L003

characteristic: Submitter Id

characteristic: anonymized name

text: Southwest

characteristic: basin

characteristic: conductivity

characteristic: depth

characteristic: description

text: TRUE

text: large lake biome

text: freshwater lake

text: water

text: fresh water

text: TRUE

text: Germany:Fuchskuhle Lake

text: Fuchskuhle

text: freshwater metagenome

characteristic: organism

text: Missing: Not provided

text: ANL

text: TRUE

text: TRUE

text: Missing: Not provided

text: fresh water

text: freshwater metagenome

text: Routine samples of German Lakes

characteristic: title


characteristic: ENA-CHECKLIST

text: UCSDMI

text: public

text: qiita_sid_945:945.P9.H7.lane3.NoIndex.L003

text: deepest point

text: freshwater metagenome, Lake Melzer

text: TRUE

characteristic: elevation

text: large lake biome

characteristic: env biome

text: freshwater lake

characteristic: env feature

text: water

characteristic: env package

text: fresh water

text: TRUE

characteristic: extracted DNA avail now

text: Germany:Lake Melzer Lake

characteristic: geo loc name

characteristic: host subject id

text: Lake Melzer

characteristic: lake

characteristic: latitude

characteristic: longitude

text: freshwater metagenome

characteristic: oxygen

characteristic: ph

text: Missing: Not provided

text: ANL

text: TRUE

text: TRUE

characteristic: public

text: Missing: Not provided

text: fresh water

characteristic: sample type

text: freshwater metagenome

characteristic: secchi depth

characteristic: temp

text: Routine samples of German Lakes


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: qiita_sid_945:945.P9.H8.lane3.NoIndex.L003

characteristic: Submitter Id

characteristic: anonymized name

text: deepest point

characteristic: basin

characteristic: conductivity

characteristic: depth

text: freshwater metagenome, Lake Melzer

characteristic: description

text: TRUE

text: large lake biome

text: freshwater lake

text: water

text: fresh water

text: TRUE

text: Germany:Lake Melzer Lake

text: Lake Melzer

text: freshwater metagenome

characteristic: organism

text: Missing: Not provided

text: ANL

text: TRUE

text: TRUE

text: Missing: Not provided

text: fresh water

text: freshwater metagenome

text: Routine samples of German Lakes

characteristic: title


characteristic: ENA-CHECKLIST

text: UCSDMI

text: public

text: qiita_sid_945:945.P9.H9.lane3.NoIndex.L003

text: deepest point

text: freshwater metagenome, Lake Melzer

text: TRUE

characteristic: elevation

text: large lake biome

characteristic: env biome

text: freshwater lake

characteristic: env feature

text: water

characteristic: env package

text: fresh water

text: TRUE

characteristic: extracted DNA avail now

text: Germany:Lake Melzer Lake

characteristic: geo loc name

characteristic: host subject id

text: Lake Melzer

characteristic: lake

characteristic: latitude

characteristic: longitude

text: freshwater metagenome

characteristic: oxygen

characteristic: ph

text: Missing: Not provided

text: ANL

text: TRUE

text: TRUE

characteristic: public

text: Missing: Not provided

text: fresh water

characteristic: sample type

text: freshwater metagenome

characteristic: secchi depth

characteristic: temp

text: Routine samples of German Lakes


text: Plasmodium relictum

characteristic: ArrayExpress-SPECIES

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Plasmodium relictum

characteristic: common name

text: Plasmodium relictum

characteristic: organism

text: Plasmodium relictum

characteristic: subject id

characteristic: title


text: public

tag: Namespace:MAX PLANCK INSTITUTE FOR PLANT BREEDING RESEARCH

text: thale cress

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: wildtype Col-0


text: MAX PLANCK INSTITUTE FOR PLANT BREEDING RESEARCH

text: public

text: thale cress

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: wildtype Col-0


text: public

text: thale cress

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: mutant lhp1-3


text: public

text: thale cress

text: Arabidopsis thaliana

text: Arabidopsis thaliana

text: double mutant eol1-1 lhp1-3


text: UCSDMI

text: public

characteristic: anonymized name

text: no

text: 2015-10-05 13:00

text: stool from mice

characteristic: description

text: TRUE

characteristic: elevation

text: urban biome

characteristic: env biome

text: animal-associated habitat

characteristic: env feature

text: host-associated

characteristic: env package

text: feces

text: USA:CA:Davis

characteristic: geo loc name

text: mouse

characteristic: host common name

text: Mus musculus

characteristic: host scientific name

characteristic: host subject id

characteristic: host taxid

characteristic: latitude

characteristic: longitude

text: mouse gut metagenome

text: Marco lab

text: FALSE

text: stool

characteristic: sample type

text: mouse gut metagenome

text: The relevance of sucrose metabolism on Lactobacillus in vivo fitness


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: no

text: 2015-10-05 13:00

text: stool from mice

text: TRUE

text: urban biome

text: animal-associated habitat

text: host-associated

text: feces

text: USA:CA:Davis

text: mouse

text: Mus musculus

text: mouse gut metagenome

characteristic: organism

text: Marco lab

text: FALSE

text: stool

text: mouse gut metagenome


text: UCSDMI

text: public

characteristic: anonymized name

text: 2015-10-09 13:00

text: cecal content from mice

characteristic: description

text: TRUE

characteristic: elevation

text: urban biome

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text: animal-associated habitat

characteristic: env feature

text: host-associated

characteristic: env package

text: organic material

text: USA:CA:Davis

characteristic: geo loc name

text: mouse

characteristic: host common name

text: Mus musculus

characteristic: host scientific name

text: mouse gut metagenome

text: Marco lab

text: TRUE

text: cecal content

text: mouse gut metagenome

characteristic: title

text: plantaricin effect on gut microbiota


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

text: public

text: 2015-10-07 10:00

text: stool from mice

text: TRUE

text: urban biome

text: animal-associated habitat

text: host-associated

text: feces

text: USA:CA:Davis

text: mouse

text: Mus musculus

characteristic: host subject id

characteristic: host taxid

characteristic: latitude

characteristic: longitude

text: mouse gut metagenome

text: Marco lab

text: FALSE

text: stool

characteristic: sample type

text: mouse gut metagenome

text: plantaricin effect on gut microbiota


text: UCSDMI

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: anonymized name

text: 2015-10-09 13:00

text: cecal content from mice

characteristic: description

text: TRUE

characteristic: elevation

text: urban biome

characteristic: env biome

text: animal-associated habitat

text: host-associated

text: organic material

text: USA:CA:Davis

text: mouse

text: Mus musculus

text: mouse gut metagenome

characteristic: organism

text: Marco lab

text: TRUE

text: cecal content

text: mouse gut metagenome

text: plantaricin effect on gut microbiota


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INSTITUT PASTEUR

text: public

tag: Namespace:INSTITUT PASTEUR

text: Listeria monocytogenes isolated from fresh leaves in a food market from Nigeria

text: Listeria monocytogenes

text: Listeria monocytogenes

text: Listeria monocytogenes LmNG1

characteristic: title


text: INSTITUT PASTEUR

characteristic: INSDC center name

text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: Listeria monocytogenes LmNG2


text: INSTITUT PASTEUR

text: public

text: Listeria monocytogenes

text: Listeria monocytogenes

text: Listeria monocytogenes LmNG3


text: Cancer Research UK Cambridge Institute

text: public

text: E-MTAB-5522:SLX-9555.N701_S502.C89V9ANXX.s_1.r_1

tag: Namespace:Cancer Research UK Cambridge Institute

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: embryonic stem cell

characteristic: cell type

text: house mouse

characteristic: common name

text: Protocols: The murine multipotent myeloid progenitor cell line 416B was stably transduced with a TetOn construct of the Cbfb-Myh11 (CM) oncogene (type A cDNA), using an in-frame F2A-mCherry protein as a reporter. As a control, cells were alternatively transduced with a version of the construct lacking the CM cDNA. 416B cells were maintained in RPMI medium, supplemented with 10% fetal calf serum and antibiotics. Expression of the CM oncogene or mCherry was induced by treatment with 1 ug/ml of doxycycline, and induction was confirmed after 24 hours by measurement of mCherry levels by fluorescence activated cell sorting. Reverse transcription was performed in a final volume of 13.2 ul per well, containing 1 uM of oligo-dT (Sigma-Aldrich), 1.04 mM of each dNTP (ThermoFisher), 100 U of SuperScript II retrotranscriptase (Invitrogen/ThermoFisher), 5 U of RNase inhibitor (Ambion), 5 mM of DTT, 1 M of Betaine (Sigma-Alrich), 6 mM of MgCl$_2$ (Ambion) and 1 uM of TSO primer (Exiqon). Spike-in RNA was added such that each well contained a final volume of 0.1 ul of a 1:3,000,000 dilution of the ERCC RNA Spike-In Mix (Invitrogen/ThermoFisher) and 0.12 ul of a 1:3,000,000 dilution of the Spike-in RNA Variant (SIRV) Control Mix E0 (Lexogen). Cells were sorted into individual wells of a 96-well microtiter plate. Each well contained 2.3 ul of lysis buffer with RNAse inhibitor (Ambion) in a 0.2% (v/v) Triton X-100 solution. Preamplification was performed in a total volume of 27 ul that contained 13.5 ul of HiFi Hotstart ReadyMix (2x; KAPA Biosystems) and 0.1 uM of IS PCR primer (Sigma-Aldrich). After 23 cycles of amplification, samples were cleaned with 80% (v/v) of Ampure beads (Beckman Coulter). Sequencing libraries were prepared using the Nextera XT DNA sample preparation kit (Illumina).

text: Doxycycline-inducible CBFB-MYH11 oncogene

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: wild type phenotype

characteristic: phenotype

text: Mus musculus

text: OK

characteristic: single cell well quality

characteristic: strain


text: Cancer Research UK Cambridge Institute

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-5522:SLX-9555.N701_S503.C89V9ANXX.s_1.r_1

characteristic: Submitter Id

text: ArrayExpress

text: embryonic stem cell

text: house mouse

characteristic: description

text: Mus musculus

text: Mus musculus

text: wild type phenotype

text: Mus musculus

text: OK


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Cancer Research UK Cambridge Institute

text: public

text: E-MTAB-5522:SLX-9555.N701_S504.C89V9ANXX.s_1.r_1

text: ArrayExpress

text: embryonic stem cell

text: house mouse

text: Mus musculus

characteristic: organism

text: Mus musculus

text: wild type phenotype

text: Mus musculus

text: OK


text: Cancer Research UK Cambridge Institute

characteristic: INSDC center name

text: public

text: E-MTAB-5522:SLX-9555.N701_S505.C89V9ANXX.s_1.r_1

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: embryonic stem cell

characteristic: cell type

text: house mouse

text: Mus musculus

text: Mus musculus

text: induced CBFB-MYH11 oncogene expression

text: Mus musculus

text: OK

characteristic: title


text: Cancer Research UK Cambridge Institute

text: public

text: E-MTAB-5522:SLX-9555.N701_S506.C89V9ANXX.s_1.r_1

text: ArrayExpress

text: embryonic stem cell

text: house mouse

characteristic: common name

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: induced CBFB-MYH11 oncogene expression

characteristic: phenotype

text: Mus musculus

text: OK

characteristic: single cell well quality

characteristic: strain


text: Cancer Research UK Cambridge Institute

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-5522:SLX-9555.N701_S507.C89V9ANXX.s_1.r_1

characteristic: Submitter Id

text: ArrayExpress

text: embryonic stem cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: induced CBFB-MYH11 oncogene expression

text: Mus musculus

text: OK


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Cancer Research UK Cambridge Institute

text: public

text: E-MTAB-5522:SLX-9555.N701_S508.C89V9ANXX.s_1.r_1

text: ArrayExpress

text: embryonic stem cell

text: house mouse

characteristic: description

text: Mus musculus

text: Mus musculus

text: induced CBFB-MYH11 oncogene expression

text: Mus musculus

text: OK


text: Cancer Research UK Cambridge Institute

characteristic: INSDC center name

text: public

text: E-MTAB-5522:SLX-9555.N701_S517.C89V9ANXX.s_1.r_1

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: embryonic stem cell

characteristic: cell type

text: house mouse

text: Mus musculus

characteristic: organism

text: Mus musculus

text: wild type phenotype

text: Mus musculus

text: OK


text: Cancer Research UK Cambridge Institute

text: public

text: E-MTAB-5522:SLX-9555.N702_S502.C89V9ANXX.s_1.r_1

text: ArrayExpress

text: embryonic stem cell

text: house mouse

characteristic: common name

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: wild type phenotype

characteristic: phenotype

text: Mus musculus

text: OK

characteristic: single cell well quality

characteristic: title


text: Cancer Research UK Cambridge Institute

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-5522:SLX-9555.N702_S503.C89V9ANXX.s_1.r_1

text: ArrayExpress

text: embryonic stem cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: wild type phenotype

text: Mus musculus

text: OK

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Cancer Research UK Cambridge Institute

text: public

text: E-MTAB-5522:SLX-9555.N702_S504.C89V9ANXX.s_1.r_1

characteristic: Submitter Id

text: ArrayExpress

text: embryonic stem cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: wild type phenotype

text: Mus musculus

text: OK


text: Cancer Research UK Cambridge Institute

characteristic: INSDC center name

text: public

text: E-MTAB-5522:SLX-9555.N702_S505.C89V9ANXX.s_1.r_1

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: embryonic stem cell

characteristic: cell type

text: house mouse

characteristic: description

text: Mus musculus

text: Mus musculus

text: induced CBFB-MYH11 oncogene expression

text: Mus musculus

text: OK


text: Cancer Research UK Cambridge Institute

text: public

text: E-MTAB-5522:SLX-9555.N702_S506.C89V9ANXX.s_1.r_1

text: ArrayExpress

text: embryonic stem cell

text: house mouse

characteristic: common name

characteristic: genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: induced CBFB-MYH11 oncogene expression

characteristic: phenotype

text: Mus musculus

text: OK

characteristic: single cell well quality


text: Cancer Research UK Cambridge Institute

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-5522:SLX-9555.N702_S507.C89V9ANXX.s_1.r_1

text: ArrayExpress

text: embryonic stem cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: induced CBFB-MYH11 oncogene expression

text: Mus musculus

text: OK

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Cancer Research UK Cambridge Institute

text: public

text: E-MTAB-5522:SLX-9555.N702_S508.C89V9ANXX.s_1.r_1

text: ArrayExpress

text: embryonic stem cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: induced CBFB-MYH11 oncogene expression

text: Mus musculus

text: OK

characteristic: strain


text: Cancer Research UK Cambridge Institute

characteristic: INSDC center name

text: public

text: E-MTAB-5522:SLX-9555.N702_S517.C89V9ANXX.s_1.r_1

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: embryonic stem cell

characteristic: cell type

text: house mouse

text: Mus musculus

text: Mus musculus

text: wild type phenotype

text: Mus musculus

text: OK


text: Cancer Research UK Cambridge Institute

text: public

text: E-MTAB-5522:SLX-9555.N703_S502.C89V9ANXX.s_1.r_1

text: ArrayExpress

text: embryonic stem cell

text: house mouse

characteristic: common name

text: Protocols: The murine multipotent myeloid progenitor cell line 416B was stably transduced with a TetOn construct of the Cbfb-Myh11 (CM) oncogene (type A cDNA), using an in-frame F2A-mCherry protein as a reporter. As a control, cells were alternatively transduced with a version of the construct lacking the CM cDNA. 416B cells were maintained in RPMI medium, supplemented with 10% fetal calf serum and antibiotics. Expression of the CM oncogene or mCherry was induced by treatment with 1 ug/ml of doxycycline, and induction was confirmed after 24 hours by measurement of mCherry levels by fluorescence activated cell sorting. Reverse transcription was performed in a final volume of 13.2 ul per well, containing 1 uM of oligo-dT (Sigma-Aldrich), 1.04 mM of each dNTP (ThermoFisher), 100 U of SuperScript II retrotranscriptase (Invitrogen/ThermoFisher), 5 U of RNase inhibitor (Ambion), 5 mM of DTT, 1 M of Betaine (Sigma-Alrich), 6 mM of MgCl$_2$ (Ambion) and 1 uM of TSO primer (Exiqon). Spike-in RNA was added such that each well contained a final volume of 0.1 ul of a 1:3,000,000 dilution of the ERCC RNA Spike-In Mix (Invitrogen/ThermoFisher) and 0.12 ul of a 1:3,000,000 dilution of the Spike-in RNA Variant (SIRV) Control Mix E0 (Lexogen). Cells were sorted into individual wells of a 96-well microtiter plate. Each well contained 2.3 ul of lysis buffer with RNAse inhibitor (Ambion) in a 0.2% (v/v) Triton X-100 solution. Preamplification was performed in a total volume of 27 ul that contained 13.5 ul of HiFi Hotstart ReadyMix (2x; KAPA Biosystems) and 0.1 uM of IS PCR primer (Sigma-Aldrich). After 23 cycles of amplification, samples were cleaned with 80% (v/v) of Ampure beads (Beckman Coulter). Sequencing libraries were prepared using the Nextera XT DNA sample preparation kit (Illumina).

characteristic: description

characteristic: genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: wild type phenotype

characteristic: phenotype

text: Mus musculus

text: OK


text: Cancer Research UK Cambridge Institute

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-5522:SLX-9555.N703_S503.C89V9ANXX.s_1.r_1

text: ArrayExpress

text: embryonic stem cell

text: house mouse

text: Doxycycline-inducible CBFB-MYH11 oncogene

text: Mus musculus

text: Mus musculus

text: wild type phenotype

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Cancer Research UK Cambridge Institute

text: public

text: E-MTAB-5522:SLX-9555.N703_S504.C89V9ANXX.s_1.r_1

text: ArrayExpress

text: embryonic stem cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: wild type phenotype

text: Mus musculus

text: OK

characteristic: title


text: Cancer Research UK Cambridge Institute

characteristic: INSDC center name

text: public

text: E-MTAB-5522:SLX-9555.N703_S505.C89V9ANXX.s_1.r_1

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: embryonic stem cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: induced CBFB-MYH11 oncogene expression

text: Mus musculus

text: OK

characteristic: strain


text: Cancer Research UK Cambridge Institute

text: public

text: E-MTAB-5522:SLX-9555.N703_S506.C89V9ANXX.s_1.r_1

text: ArrayExpress

text: embryonic stem cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

characteristic: genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: induced CBFB-MYH11 oncogene expression

text: Mus musculus

text: OK


text: Cancer Research UK Cambridge Institute

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-5522:SLX-9555.N703_S507.C89V9ANXX.s_1.r_1

text: ArrayExpress

text: embryonic stem cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: induced CBFB-MYH11 oncogene expression

characteristic: phenotype

text: Mus musculus

text: OK


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Cancer Research UK Cambridge Institute

text: public

text: E-MTAB-5522:SLX-9555.N703_S508.C89V9ANXX.s_1.r_1

text: ArrayExpress

text: embryonic stem cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: induced CBFB-MYH11 oncogene expression

text: Mus musculus

text: OK

characteristic: single cell well quality


text: Cancer Research UK Cambridge Institute

characteristic: INSDC center name

text: public

text: E-MTAB-5522:SLX-9555.N703_S517.C89V9ANXX.s_1.r_1

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: embryonic stem cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: wild type phenotype

text: Mus musculus

text: OK

characteristic: title


text: Cancer Research UK Cambridge Institute

text: public

text: E-MTAB-5522:SLX-9555.N704_S502.C89V9ANXX.s_1.r_1

text: ArrayExpress

text: embryonic stem cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: wild type phenotype

text: Mus musculus

text: OK

characteristic: strain


text: Cancer Research UK Cambridge Institute

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-5522:SLX-9555.N704_S503.C89V9ANXX.s_1.r_1

text: ArrayExpress

text: embryonic stem cell

text: house mouse

text: Mus musculus

characteristic: organism

text: Mus musculus

text: wild type phenotype

text: Mus musculus

text: OK


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Cancer Research UK Cambridge Institute

text: public

text: E-MTAB-5522:SLX-9555.N704_S504.C89V9ANXX.s_1.r_1

text: ArrayExpress

text: embryonic stem cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: wild type phenotype

characteristic: phenotype

text: Mus musculus

text: OK


text: Cancer Research UK Cambridge Institute

characteristic: INSDC center name

text: public

text: E-MTAB-5522:SLX-9555.N704_S505.C89V9ANXX.s_1.r_1

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: embryonic stem cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: induced CBFB-MYH11 oncogene expression

text: Mus musculus

text: OK

characteristic: single cell well quality


text: Cancer Research UK Cambridge Institute

text: public

text: E-MTAB-5522:SLX-9555.N704_S506.C89V9ANXX.s_1.r_1

text: ArrayExpress

text: embryonic stem cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: induced CBFB-MYH11 oncogene expression

text: Mus musculus

text: OK

characteristic: title


text: Cancer Research UK Cambridge Institute

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-5522:SLX-9555.N704_S507.C89V9ANXX.s_1.r_1

text: ArrayExpress

text: embryonic stem cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: induced CBFB-MYH11 oncogene expression

text: Mus musculus

text: OK

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Cancer Research UK Cambridge Institute

text: public

text: E-MTAB-5522:SLX-9555.N704_S508.C89V9ANXX.s_1.r_1

text: ArrayExpress

text: embryonic stem cell

text: house mouse

text: Mus musculus

characteristic: organism

text: Mus musculus

text: induced CBFB-MYH11 oncogene expression

text: Mus musculus

text: OK


text: Cancer Research UK Cambridge Institute

characteristic: INSDC center name

text: public

text: E-MTAB-5522:SLX-9555.N704_S517.C89V9ANXX.s_1.r_1

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: embryonic stem cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: wild type phenotype

characteristic: phenotype

text: Mus musculus

text: OK


text: Cancer Research UK Cambridge Institute

text: public

text: E-MTAB-5522:SLX-9555.N705_S502.C89V9ANXX.s_1.r_1

text: ArrayExpress

text: embryonic stem cell

characteristic: cell type

text: house mouse

characteristic: common name

text: Protocols: The murine multipotent myeloid progenitor cell line 416B was stably transduced with a TetOn construct of the Cbfb-Myh11 (CM) oncogene (type A cDNA), using an in-frame F2A-mCherry protein as a reporter. As a control, cells were alternatively transduced with a version of the construct lacking the CM cDNA. 416B cells were maintained in RPMI medium, supplemented with 10% fetal calf serum and antibiotics. Expression of the CM oncogene or mCherry was induced by treatment with 1 ug/ml of doxycycline, and induction was confirmed after 24 hours by measurement of mCherry levels by fluorescence activated cell sorting. Reverse transcription was performed in a final volume of 13.2 ul per well, containing 1 uM of oligo-dT (Sigma-Aldrich), 1.04 mM of each dNTP (ThermoFisher), 100 U of SuperScript II retrotranscriptase (Invitrogen/ThermoFisher), 5 U of RNase inhibitor (Ambion), 5 mM of DTT, 1 M of Betaine (Sigma-Alrich), 6 mM of MgCl$_2$ (Ambion) and 1 uM of TSO primer (Exiqon). Spike-in RNA was added such that each well contained a final volume of 0.1 ul of a 1:3,000,000 dilution of the ERCC RNA Spike-In Mix (Invitrogen/ThermoFisher) and 0.12 ul of a 1:3,000,000 dilution of the Spike-in RNA Variant (SIRV) Control Mix E0 (Lexogen). Cells were sorted into individual wells of a 96-well microtiter plate. Each well contained 2.3 ul of lysis buffer with RNAse inhibitor (Ambion) in a 0.2% (v/v) Triton X-100 solution. Preamplification was performed in a total volume of 27 ul that contained 13.5 ul of HiFi Hotstart ReadyMix (2x; KAPA Biosystems) and 0.1 uM of IS PCR primer (Sigma-Aldrich). After 23 cycles of amplification, samples were cleaned with 80% (v/v) of Ampure beads (Beckman Coulter). Sequencing libraries were prepared using the Nextera XT DNA sample preparation kit (Illumina).

characteristic: description

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: wild type phenotype

text: Mus musculus

text: OK

characteristic: single cell well quality


text: Cancer Research UK Cambridge Institute

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-5522:SLX-9555.N705_S503.C89V9ANXX.s_1.r_1

text: ArrayExpress

text: embryonic stem cell

text: house mouse

text: Doxycycline-inducible CBFB-MYH11 oncogene

text: Mus musculus

text: Mus musculus

text: wild type phenotype

text: Mus musculus

text: OK

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Cancer Research UK Cambridge Institute

text: public

text: E-MTAB-5522:SLX-9555.N705_S504.C89V9ANXX.s_1.r_1

text: ArrayExpress

text: embryonic stem cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: wild type phenotype

text: Mus musculus

text: OK

characteristic: strain


text: Cancer Research UK Cambridge Institute

characteristic: INSDC center name

text: public

text: E-MTAB-5522:SLX-9555.N705_S505.C89V9ANXX.s_1.r_1

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: embryonic stem cell

text: house mouse

text: Mus musculus

characteristic: organism

text: Mus musculus

text: induced CBFB-MYH11 oncogene expression

text: Mus musculus

text: OK


text: Cancer Research UK Cambridge Institute

text: public

text: E-MTAB-5522:SLX-9555.N705_S506.C89V9ANXX.s_1.r_1

text: ArrayExpress

text: embryonic stem cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: induced CBFB-MYH11 oncogene expression

characteristic: phenotype

text: Mus musculus

text: OK


text: Cancer Research UK Cambridge Institute

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-5522:SLX-9555.N705_S507.C89V9ANXX.s_1.r_1

text: ArrayExpress

text: embryonic stem cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: induced CBFB-MYH11 oncogene expression

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Cancer Research UK Cambridge Institute

text: public

text: E-MTAB-5522:SLX-9555.N705_S508.C89V9ANXX.s_1.r_1

text: ArrayExpress

text: embryonic stem cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: induced CBFB-MYH11 oncogene expression

text: Mus musculus

text: OK

characteristic: title


text: Cancer Research UK Cambridge Institute

characteristic: INSDC center name

text: public

text: E-MTAB-5522:SLX-9555.N705_S517.C89V9ANXX.s_1.r_1

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: embryonic stem cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: wild type phenotype

text: Mus musculus

text: OK

characteristic: strain


text: Cancer Research UK Cambridge Institute

text: public

text: E-MTAB-5522:SLX-9555.N706_S502.C89V9ANXX.s_1.r_1

text: ArrayExpress

text: embryonic stem cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

characteristic: genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: wild type phenotype

text: Mus musculus

text: OK


text: Cancer Research UK Cambridge Institute

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-5522:SLX-9555.N706_S503.C89V9ANXX.s_1.r_1

text: ArrayExpress

text: embryonic stem cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: wild type phenotype

characteristic: phenotype

text: Mus musculus

text: OK


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Cancer Research UK Cambridge Institute

text: public

text: E-MTAB-5522:SLX-9555.N706_S504.C89V9ANXX.s_1.r_1

text: ArrayExpress

text: embryonic stem cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: wild type phenotype

text: Mus musculus

text: OK

characteristic: single cell well quality


text: Cancer Research UK Cambridge Institute

characteristic: INSDC center name

text: public

text: E-MTAB-5522:SLX-9555.N706_S505.C89V9ANXX.s_1.r_1

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: embryonic stem cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: induced CBFB-MYH11 oncogene expression

text: Mus musculus

text: OK

characteristic: title


text: Cancer Research UK Cambridge Institute

text: public

text: E-MTAB-5522:SLX-9555.N706_S506.C89V9ANXX.s_1.r_1

text: ArrayExpress

text: embryonic stem cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: induced CBFB-MYH11 oncogene expression

text: Mus musculus

text: OK

characteristic: strain


text: Cancer Research UK Cambridge Institute

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-5522:SLX-9555.N706_S507.C89V9ANXX.s_1.r_1

text: ArrayExpress

text: embryonic stem cell

text: house mouse

text: Mus musculus

characteristic: organism

text: Mus musculus

text: induced CBFB-MYH11 oncogene expression

text: Mus musculus

text: OK


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Cancer Research UK Cambridge Institute

text: public

text: E-MTAB-5522:SLX-9555.N706_S508.C89V9ANXX.s_1.r_1

text: ArrayExpress

text: embryonic stem cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: induced CBFB-MYH11 oncogene expression

characteristic: phenotype

text: Mus musculus

text: OK


text: Cancer Research UK Cambridge Institute

characteristic: INSDC center name

text: public

text: E-MTAB-5522:SLX-9555.N706_S517.C89V9ANXX.s_1.r_1

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: embryonic stem cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: wild type phenotype

text: Mus musculus

text: OK

characteristic: single cell well quality


text: Cancer Research UK Cambridge Institute

text: public

text: E-MTAB-5522:SLX-9555.N707_S502.C89V9ANXX.s_1.r_1

text: ArrayExpress

text: embryonic stem cell

characteristic: cell type

text: house mouse

characteristic: common name

text: Protocols: The murine multipotent myeloid progenitor cell line 416B was stably transduced with a TetOn construct of the Cbfb-Myh11 (CM) oncogene (type A cDNA), using an in-frame F2A-mCherry protein as a reporter. As a control, cells were alternatively transduced with a version of the construct lacking the CM cDNA. 416B cells were maintained in RPMI medium, supplemented with 10% fetal calf serum and antibiotics. Expression of the CM oncogene or mCherry was induced by treatment with 1 ug/ml of doxycycline, and induction was confirmed after 24 hours by measurement of mCherry levels by fluorescence activated cell sorting. Reverse transcription was performed in a final volume of 13.2 ul per well, containing 1 uM of oligo-dT (Sigma-Aldrich), 1.04 mM of each dNTP (ThermoFisher), 100 U of SuperScript II retrotranscriptase (Invitrogen/ThermoFisher), 5 U of RNase inhibitor (Ambion), 5 mM of DTT, 1 M of Betaine (Sigma-Alrich), 6 mM of MgCl$_2$ (Ambion) and 1 uM of TSO primer (Exiqon). Spike-in RNA was added such that each well contained a final volume of 0.1 ul of a 1:3,000,000 dilution of the ERCC RNA Spike-In Mix (Invitrogen/ThermoFisher) and 0.12 ul of a 1:3,000,000 dilution of the Spike-in RNA Variant (SIRV) Control Mix E0 (Lexogen). Cells were sorted into individual wells of a 96-well microtiter plate. Each well contained 2.3 ul of lysis buffer with RNAse inhibitor (Ambion) in a 0.2% (v/v) Triton X-100 solution. Preamplification was performed in a total volume of 27 ul that contained 13.5 ul of HiFi Hotstart ReadyMix (2x; KAPA Biosystems) and 0.1 uM of IS PCR primer (Sigma-Aldrich). After 23 cycles of amplification, samples were cleaned with 80% (v/v) of Ampure beads (Beckman Coulter). Sequencing libraries were prepared using the Nextera XT DNA sample preparation kit (Illumina).

characteristic: description

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: wild type phenotype

text: Mus musculus

text: OK

characteristic: title


text: Cancer Research UK Cambridge Institute

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-5522:SLX-9555.N707_S503.C89V9ANXX.s_1.r_1

text: ArrayExpress

text: embryonic stem cell

text: house mouse

text: Doxycycline-inducible CBFB-MYH11 oncogene

text: Mus musculus

text: Mus musculus

text: wild type phenotype

text: Mus musculus

text: OK

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Cancer Research UK Cambridge Institute

text: public

text: E-MTAB-5522:SLX-9555.N707_S504.C89V9ANXX.s_1.r_1

text: ArrayExpress

text: embryonic stem cell

text: house mouse

text: Mus musculus

characteristic: organism

text: Mus musculus

text: wild type phenotype

text: Mus musculus

text: OK


text: Cancer Research UK Cambridge Institute

characteristic: INSDC center name

text: public

text: E-MTAB-5522:SLX-9555.N707_S505.C89V9ANXX.s_1.r_1

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: embryonic stem cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: induced CBFB-MYH11 oncogene expression

characteristic: phenotype

text: Mus musculus

text: OK


text: Cancer Research UK Cambridge Institute

text: public

text: E-MTAB-5522:SLX-9555.N707_S506.C89V9ANXX.s_1.r_1

text: ArrayExpress

text: embryonic stem cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: induced CBFB-MYH11 oncogene expression

text: Mus musculus

text: OK

characteristic: single cell well quality


text: Cancer Research UK Cambridge Institute

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-5522:SLX-9555.N707_S507.C89V9ANXX.s_1.r_1

text: ArrayExpress

text: embryonic stem cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: induced CBFB-MYH11 oncogene expression

text: Mus musculus

text: OK

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Cancer Research UK Cambridge Institute

text: public

text: E-MTAB-5522:SLX-9555.N707_S508.C89V9ANXX.s_1.r_1

text: ArrayExpress

text: embryonic stem cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: induced CBFB-MYH11 oncogene expression

text: Mus musculus

text: OK

characteristic: strain


text: Cancer Research UK Cambridge Institute

characteristic: INSDC center name

text: public

text: E-MTAB-5522:SLX-9555.N707_S517.C89V9ANXX.s_1.r_1

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: embryonic stem cell

text: house mouse

text: Mus musculus

characteristic: organism

text: Mus musculus

text: wild type phenotype

text: Mus musculus

text: OK


text: Cancer Research UK Cambridge Institute

text: public

text: E-MTAB-5522:SLX-9555.N708_S502.C89V9ANXX.s_1.r_1

text: ArrayExpress

text: embryonic stem cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: wild type phenotype

characteristic: phenotype

text: Mus musculus

text: OK


text: Cancer Research UK Cambridge Institute

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-5522:SLX-9555.N708_S503.C89V9ANXX.s_1.r_1

text: ArrayExpress

text: embryonic stem cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: wild type phenotype

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Cancer Research UK Cambridge Institute

text: public

text: E-MTAB-5522:SLX-9555.N708_S504.C89V9ANXX.s_1.r_1

text: ArrayExpress

text: embryonic stem cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: wild type phenotype

text: Mus musculus

text: OK

characteristic: title


text: Cancer Research UK Cambridge Institute

characteristic: INSDC center name

text: public

text: E-MTAB-5522:SLX-9555.N708_S505.C89V9ANXX.s_1.r_1

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: embryonic stem cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: induced CBFB-MYH11 oncogene expression

text: Mus musculus

text: OK

characteristic: strain


text: Cancer Research UK Cambridge Institute

text: public

text: E-MTAB-5522:SLX-9555.N708_S506.C89V9ANXX.s_1.r_1

text: ArrayExpress

text: embryonic stem cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

characteristic: genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: induced CBFB-MYH11 oncogene expression

text: Mus musculus

text: OK


text: Cancer Research UK Cambridge Institute

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-5522:SLX-9555.N708_S507.C89V9ANXX.s_1.r_1

text: ArrayExpress

text: embryonic stem cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: induced CBFB-MYH11 oncogene expression

characteristic: phenotype

text: Mus musculus

text: OK


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Cancer Research UK Cambridge Institute

text: public

text: E-MTAB-5522:SLX-9555.N708_S508.C89V9ANXX.s_1.r_1

text: ArrayExpress

text: embryonic stem cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: induced CBFB-MYH11 oncogene expression

text: Mus musculus

text: OK

characteristic: single cell well quality


text: Cancer Research UK Cambridge Institute

characteristic: INSDC center name

text: public

text: E-MTAB-5522:SLX-9555.N708_S517.C89V9ANXX.s_1.r_1

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: embryonic stem cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: wild type phenotype

text: Mus musculus

text: OK

characteristic: title


text: Cancer Research UK Cambridge Institute

text: public

text: E-MTAB-5522:SLX-9555.N709_S502.C89V9ANXX.s_1.r_1

text: ArrayExpress

text: embryonic stem cell

characteristic: cell type

text: house mouse

characteristic: common name

text: Protocols: The murine multipotent myeloid progenitor cell line 416B was stably transduced with a TetOn construct of the Cbfb-Myh11 (CM) oncogene (type A cDNA), using an in-frame F2A-mCherry protein as a reporter. As a control, cells were alternatively transduced with a version of the construct lacking the CM cDNA. 416B cells were maintained in RPMI medium, supplemented with 10% fetal calf serum and antibiotics. Expression of the CM oncogene or mCherry was induced by treatment with 1 ug/ml of doxycycline, and induction was confirmed after 24 hours by measurement of mCherry levels by fluorescence activated cell sorting. Reverse transcription was performed in a final volume of 13.2 ul per well, containing 1 uM of oligo-dT (Sigma-Aldrich), 1.04 mM of each dNTP (ThermoFisher), 100 U of SuperScript II retrotranscriptase (Invitrogen/ThermoFisher), 5 U of RNase inhibitor (Ambion), 5 mM of DTT, 1 M of Betaine (Sigma-Alrich), 6 mM of MgCl$_2$ (Ambion) and 1 uM of TSO primer (Exiqon). Spike-in RNA was added such that each well contained a final volume of 0.1 ul of a 1:3,000,000 dilution of the ERCC RNA Spike-In Mix (Invitrogen/ThermoFisher) and 0.12 ul of a 1:3,000,000 dilution of the Spike-in RNA Variant (SIRV) Control Mix E0 (Lexogen). Cells were sorted into individual wells of a 96-well microtiter plate. Each well contained 2.3 ul of lysis buffer with RNAse inhibitor (Ambion) in a 0.2% (v/v) Triton X-100 solution. Preamplification was performed in a total volume of 27 ul that contained 13.5 ul of HiFi Hotstart ReadyMix (2x; KAPA Biosystems) and 0.1 uM of IS PCR primer (Sigma-Aldrich). After 23 cycles of amplification, samples were cleaned with 80% (v/v) of Ampure beads (Beckman Coulter). Sequencing libraries were prepared using the Nextera XT DNA sample preparation kit (Illumina).

characteristic: description

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: wild type phenotype

text: Mus musculus

text: OK

characteristic: strain


text: Cancer Research UK Cambridge Institute

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-5522:SLX-9555.N709_S503.C89V9ANXX.s_1.r_1

text: ArrayExpress

text: embryonic stem cell

text: house mouse

text: Doxycycline-inducible CBFB-MYH11 oncogene

text: Mus musculus

characteristic: organism

text: Mus musculus

text: wild type phenotype

text: Mus musculus

text: OK


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Cancer Research UK Cambridge Institute

text: public

text: E-MTAB-5522:SLX-9555.N709_S504.C89V9ANXX.s_1.r_1

text: ArrayExpress

text: embryonic stem cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: wild type phenotype

characteristic: phenotype

text: Mus musculus

text: OK


text: Cancer Research UK Cambridge Institute

characteristic: INSDC center name

text: public

text: E-MTAB-5522:SLX-9555.N709_S505.C89V9ANXX.s_1.r_1

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: embryonic stem cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: induced CBFB-MYH11 oncogene expression

text: Mus musculus

text: OK

characteristic: single cell well quality


text: Cancer Research UK Cambridge Institute

text: public

text: E-MTAB-5522:SLX-9555.N709_S506.C89V9ANXX.s_1.r_1

text: ArrayExpress

text: embryonic stem cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: induced CBFB-MYH11 oncogene expression

text: Mus musculus

text: OK

characteristic: title


text: Cancer Research UK Cambridge Institute

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-5522:SLX-9555.N709_S507.C89V9ANXX.s_1.r_1

text: ArrayExpress

text: embryonic stem cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: induced CBFB-MYH11 oncogene expression

text: Mus musculus

text: OK

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Cancer Research UK Cambridge Institute

text: public

text: E-MTAB-5522:SLX-9555.N709_S508.C89V9ANXX.s_1.r_1

text: ArrayExpress

text: embryonic stem cell

text: house mouse

text: Mus musculus

characteristic: organism

text: Mus musculus

text: induced CBFB-MYH11 oncogene expression

text: Mus musculus

text: OK


text: Cancer Research UK Cambridge Institute

characteristic: INSDC center name

text: public

text: E-MTAB-5522:SLX-9555.N709_S517.C89V9ANXX.s_1.r_1

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: embryonic stem cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: wild type phenotype

characteristic: phenotype

text: Mus musculus

text: OK


text: Cancer Research UK Cambridge Institute

text: public

text: E-MTAB-5522:SLX-9555.N710_S502.C89V9ANXX.s_1.r_1

text: ArrayExpress

text: embryonic stem cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: wild type phenotype

text: Mus musculus

text: OK

characteristic: single cell well quality


text: Cancer Research UK Cambridge Institute

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-5522:SLX-9555.N710_S503.C89V9ANXX.s_1.r_1

text: ArrayExpress

text: embryonic stem cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: wild type phenotype

text: Mus musculus

text: OK

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Cancer Research UK Cambridge Institute

text: public

text: E-MTAB-5522:SLX-9555.N710_S504.C89V9ANXX.s_1.r_1

text: ArrayExpress

text: embryonic stem cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: wild type phenotype

text: Mus musculus

text: OK

characteristic: strain


text: Cancer Research UK Cambridge Institute

characteristic: INSDC center name

text: public

text: E-MTAB-5522:SLX-9555.N710_S505.C89V9ANXX.s_1.r_1

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: embryonic stem cell

text: house mouse

text: Mus musculus

characteristic: organism

text: Mus musculus

text: induced CBFB-MYH11 oncogene expression

text: Mus musculus

text: OK


text: Cancer Research UK Cambridge Institute

text: public

text: E-MTAB-5522:SLX-9555.N710_S506.C89V9ANXX.s_1.r_1

text: ArrayExpress

text: embryonic stem cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: induced CBFB-MYH11 oncogene expression

characteristic: phenotype

text: Mus musculus

text: OK


text: Cancer Research UK Cambridge Institute

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-5522:SLX-9555.N710_S507.C89V9ANXX.s_1.r_1

text: ArrayExpress

text: embryonic stem cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: induced CBFB-MYH11 oncogene expression

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Cancer Research UK Cambridge Institute

text: public

text: E-MTAB-5522:SLX-9555.N710_S508.C89V9ANXX.s_1.r_1

text: ArrayExpress

text: embryonic stem cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: induced CBFB-MYH11 oncogene expression

text: Mus musculus

text: OK

characteristic: title


text: Cancer Research UK Cambridge Institute

characteristic: INSDC center name

text: public

text: E-MTAB-5522:SLX-9555.N710_S517.C89V9ANXX.s_1.r_1

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: embryonic stem cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: wild type phenotype

text: Mus musculus

text: OK

characteristic: strain


text: Cancer Research UK Cambridge Institute

text: public

text: E-MTAB-5522:SLX-9555.N711_S502.C89V9ANXX.s_1.r_1

text: ArrayExpress

text: embryonic stem cell

characteristic: cell type

text: house mouse

characteristic: common name

text: Protocols: The murine multipotent myeloid progenitor cell line 416B was stably transduced with a TetOn construct of the Cbfb-Myh11 (CM) oncogene (type A cDNA), using an in-frame F2A-mCherry protein as a reporter. As a control, cells were alternatively transduced with a version of the construct lacking the CM cDNA. 416B cells were maintained in RPMI medium, supplemented with 10% fetal calf serum and antibiotics. Expression of the CM oncogene or mCherry was induced by treatment with 1 ug/ml of doxycycline, and induction was confirmed after 24 hours by measurement of mCherry levels by fluorescence activated cell sorting. Reverse transcription was performed in a final volume of 13.2 ul per well, containing 1 uM of oligo-dT (Sigma-Aldrich), 1.04 mM of each dNTP (ThermoFisher), 100 U of SuperScript II retrotranscriptase (Invitrogen/ThermoFisher), 5 U of RNase inhibitor (Ambion), 5 mM of DTT, 1 M of Betaine (Sigma-Alrich), 6 mM of MgCl$_2$ (Ambion) and 1 uM of TSO primer (Exiqon). Spike-in RNA was added such that each well contained a final volume of 0.1 ul of a 1:3,000,000 dilution of the ERCC RNA Spike-In Mix (Invitrogen/ThermoFisher) and 0.12 ul of a 1:3,000,000 dilution of the Spike-in RNA Variant (SIRV) Control Mix E0 (Lexogen). Cells were sorted into individual wells of a 96-well microtiter plate. Each well contained 2.3 ul of lysis buffer with RNAse inhibitor (Ambion) in a 0.2% (v/v) Triton X-100 solution. Preamplification was performed in a total volume of 27 ul that contained 13.5 ul of HiFi Hotstart ReadyMix (2x; KAPA Biosystems) and 0.1 uM of IS PCR primer (Sigma-Aldrich). After 23 cycles of amplification, samples were cleaned with 80% (v/v) of Ampure beads (Beckman Coulter). Sequencing libraries were prepared using the Nextera XT DNA sample preparation kit (Illumina).

characteristic: description

characteristic: genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: wild type phenotype

text: Mus musculus

text: OK


text: Cancer Research UK Cambridge Institute

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-5522:SLX-9555.N711_S503.C89V9ANXX.s_1.r_1

text: ArrayExpress

text: embryonic stem cell

text: house mouse

text: Doxycycline-inducible CBFB-MYH11 oncogene

text: Mus musculus

text: Mus musculus

text: wild type phenotype

characteristic: phenotype

text: Mus musculus

text: OK


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Cancer Research UK Cambridge Institute

text: public

text: E-MTAB-5522:SLX-9555.N711_S504.C89V9ANXX.s_1.r_1

text: ArrayExpress

text: embryonic stem cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: wild type phenotype

text: Mus musculus

text: OK

characteristic: single cell well quality


text: Cancer Research UK Cambridge Institute

characteristic: INSDC center name

text: public

text: E-MTAB-5522:SLX-9555.N711_S505.C89V9ANXX.s_1.r_1

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: embryonic stem cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: induced CBFB-MYH11 oncogene expression

text: Mus musculus

text: OK

characteristic: title


text: Cancer Research UK Cambridge Institute

text: public

text: E-MTAB-5522:SLX-9555.N711_S506.C89V9ANXX.s_1.r_1

text: ArrayExpress

text: embryonic stem cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: induced CBFB-MYH11 oncogene expression

text: Mus musculus

text: OK

characteristic: strain


text: Cancer Research UK Cambridge Institute

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-5522:SLX-9555.N711_S507.C89V9ANXX.s_1.r_1

text: ArrayExpress

text: embryonic stem cell

text: house mouse

text: Mus musculus

characteristic: organism

text: Mus musculus

text: induced CBFB-MYH11 oncogene expression

text: Mus musculus

text: OK


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Cancer Research UK Cambridge Institute

text: public

text: E-MTAB-5522:SLX-9555.N711_S508.C89V9ANXX.s_1.r_1

text: ArrayExpress

text: embryonic stem cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: induced CBFB-MYH11 oncogene expression

characteristic: phenotype

text: Mus musculus

text: OK


text: Cancer Research UK Cambridge Institute

characteristic: INSDC center name

text: public

text: E-MTAB-5522:SLX-9555.N711_S517.C89V9ANXX.s_1.r_1

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: embryonic stem cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: wild type phenotype

text: Mus musculus

text: OK

characteristic: single cell well quality


text: Cancer Research UK Cambridge Institute

text: public

text: E-MTAB-5522:SLX-9555.N712_S502.C89V9ANXX.s_1.r_1

text: ArrayExpress

text: embryonic stem cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: wild type phenotype

text: Mus musculus

text: OK

characteristic: title


text: Cancer Research UK Cambridge Institute

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-5522:SLX-9555.N712_S503.C89V9ANXX.s_1.r_1

text: ArrayExpress

text: embryonic stem cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: wild type phenotype

text: Mus musculus

text: OK

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Cancer Research UK Cambridge Institute

text: public

text: E-MTAB-5522:SLX-9555.N712_S504.C89V9ANXX.s_1.r_1

text: ArrayExpress

text: embryonic stem cell

text: house mouse

text: Mus musculus

characteristic: organism

text: Mus musculus

text: wild type phenotype

text: Mus musculus

text: OK


text: Cancer Research UK Cambridge Institute

characteristic: INSDC center name

text: public

text: E-MTAB-5522:SLX-9555.N712_S505.C89V9ANXX.s_1.r_1

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: embryonic stem cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: induced CBFB-MYH11 oncogene expression

characteristic: phenotype

text: Mus musculus

text: OK


text: Cancer Research UK Cambridge Institute

text: public

text: E-MTAB-5522:SLX-9555.N712_S506.C89V9ANXX.s_1.r_1

text: ArrayExpress

text: embryonic stem cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: induced CBFB-MYH11 oncogene expression

text: Mus musculus

text: OK

characteristic: single cell well quality


text: Cancer Research UK Cambridge Institute

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-5522:SLX-9555.N712_S507.C89V9ANXX.s_1.r_1

text: ArrayExpress

text: embryonic stem cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: induced CBFB-MYH11 oncogene expression

text: Mus musculus

text: OK

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Cancer Research UK Cambridge Institute

text: public

text: E-MTAB-5522:SLX-9555.N712_S508.C89V9ANXX.s_1.r_1

text: ArrayExpress

text: embryonic stem cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: induced CBFB-MYH11 oncogene expression

text: Mus musculus

text: OK

characteristic: strain


text: Cancer Research UK Cambridge Institute

characteristic: INSDC center name

text: public

text: E-MTAB-5522:SLX-9555.N712_S517.C89V9ANXX.s_1.r_1

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: embryonic stem cell

text: house mouse

text: Mus musculus

characteristic: organism

text: Mus musculus

text: wild type phenotype

text: Mus musculus

text: OK


text: Cancer Research UK Cambridge Institute

text: public

text: E-MTAB-5522:SLX-11312.N701_S502.H5H5YBBXX.s_8.r_1

text: ArrayExpress

text: embryonic stem cell

characteristic: cell type

text: house mouse

characteristic: common name

text: Protocols: The murine multipotent myeloid progenitor cell line 416B was stably transduced with a TetOn construct of the Cbfb-Myh11 (CM) oncogene (type A cDNA), using an in-frame F2A-mCherry protein as a reporter. As a control, cells were alternatively transduced with a version of the construct lacking the CM cDNA. 416B cells were maintained in RPMI medium, supplemented with 10% fetal calf serum and antibiotics. Expression of the CM oncogene or mCherry was induced by treatment with 1 ug/ml of doxycycline, and induction was confirmed after 24 hours by measurement of mCherry levels by fluorescence activated cell sorting. Reverse transcription was performed in a final volume of 13.2 ul per well, containing 1 uM of oligo-dT (Sigma-Aldrich), 1.04 mM of each dNTP (ThermoFisher), 100 U of SuperScript II retrotranscriptase (Invitrogen/ThermoFisher), 5 U of RNase inhibitor (Ambion), 5 mM of DTT, 1 M of Betaine (Sigma-Alrich), 6 mM of MgCl$_2$ (Ambion) and 1 uM of TSO primer (Exiqon). Spike-in RNA was added such that each well contained a final volume of 0.1 ul of a 1:3,000,000 dilution of the ERCC RNA Spike-In Mix (Invitrogen/ThermoFisher) and 0.12 ul of a 1:3,000,000 dilution of the Spike-in RNA Variant (SIRV) Control Mix E0 (Lexogen). Cells were sorted into individual wells of a 96-well microtiter plate. Each well contained 2.3 ul of lysis buffer with RNAse inhibitor (Ambion) in a 0.2% (v/v) Triton X-100 solution. Preamplification was performed in a total volume of 27 ul that contained 13.5 ul of HiFi Hotstart ReadyMix (2x; KAPA Biosystems) and 0.1 uM of IS PCR primer (Sigma-Aldrich). After 23 cycles of amplification, samples were cleaned with 80% (v/v) of Ampure beads (Beckman Coulter). Sequencing libraries were prepared using the Nextera XT DNA sample preparation kit (Illumina).

characteristic: description

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: wild type phenotype

characteristic: phenotype

text: Mus musculus

text: OK


text: Cancer Research UK Cambridge Institute

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-5522:SLX-11312.N701_S503.H5H5YBBXX.s_8.r_1

text: ArrayExpress

text: embryonic stem cell

text: house mouse

text: Doxycycline-inducible CBFB-MYH11 oncogene

text: Mus musculus

text: Mus musculus

text: wild type phenotype

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Cancer Research UK Cambridge Institute

text: public

text: E-MTAB-5522:SLX-11312.N701_S504.H5H5YBBXX.s_8.r_1

text: ArrayExpress

text: embryonic stem cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: wild type phenotype

text: Mus musculus

text: OK

characteristic: title


text: Cancer Research UK Cambridge Institute

characteristic: INSDC center name

text: public

text: E-MTAB-5522:SLX-11312.N701_S505.H5H5YBBXX.s_8.r_1

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: embryonic stem cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: induced CBFB-MYH11 oncogene expression

text: Mus musculus

text: OK

characteristic: strain


text: Cancer Research UK Cambridge Institute

text: public

text: E-MTAB-5522:SLX-11312.N701_S506.H5H5YBBXX.s_8.r_1

text: ArrayExpress

text: embryonic stem cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

characteristic: genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: induced CBFB-MYH11 oncogene expression

text: Mus musculus

text: OK


text: Cancer Research UK Cambridge Institute

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-5522:SLX-11312.N701_S507.H5H5YBBXX.s_8.r_1

text: ArrayExpress

text: embryonic stem cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: induced CBFB-MYH11 oncogene expression

characteristic: phenotype

text: Mus musculus

text: OK


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Cancer Research UK Cambridge Institute

text: public

text: E-MTAB-5522:SLX-11312.N701_S508.H5H5YBBXX.s_8.r_1

text: ArrayExpress

text: embryonic stem cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: induced CBFB-MYH11 oncogene expression

text: Mus musculus

text: OK

characteristic: single cell well quality


text: Cancer Research UK Cambridge Institute

characteristic: INSDC center name

text: public

text: E-MTAB-5522:SLX-11312.N701_S517.H5H5YBBXX.s_8.r_1

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: embryonic stem cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: wild type phenotype

text: Mus musculus

text: OK

characteristic: title


text: Cancer Research UK Cambridge Institute

text: public

text: E-MTAB-5522:SLX-11312.N702_S502.H5H5YBBXX.s_8.r_1

text: ArrayExpress

text: embryonic stem cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: wild type phenotype

text: Mus musculus

text: OK

characteristic: strain


text: Cancer Research UK Cambridge Institute

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-5522:SLX-11312.N702_S503.H5H5YBBXX.s_8.r_1

text: ArrayExpress

text: embryonic stem cell

text: house mouse

text: Mus musculus

characteristic: organism

text: Mus musculus

text: wild type phenotype

text: Mus musculus

text: OK


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Cancer Research UK Cambridge Institute

text: public

text: E-MTAB-5522:SLX-11312.N702_S504.H5H5YBBXX.s_8.r_1

text: ArrayExpress

text: embryonic stem cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: wild type phenotype

characteristic: phenotype

text: Mus musculus

text: OK


text: Cancer Research UK Cambridge Institute

characteristic: INSDC center name

text: public

text: E-MTAB-5522:SLX-11312.N702_S505.H5H5YBBXX.s_8.r_1

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: embryonic stem cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: induced CBFB-MYH11 oncogene expression

text: Mus musculus

text: OK

characteristic: single cell well quality


text: Cancer Research UK Cambridge Institute

text: public

text: E-MTAB-5522:SLX-11312.N702_S506.H5H5YBBXX.s_8.r_1

text: ArrayExpress

text: embryonic stem cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: induced CBFB-MYH11 oncogene expression

text: Mus musculus

text: OK

characteristic: title


text: Cancer Research UK Cambridge Institute

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-5522:SLX-11312.N702_S507.H5H5YBBXX.s_8.r_1

text: ArrayExpress

text: embryonic stem cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: induced CBFB-MYH11 oncogene expression

text: Mus musculus

text: OK

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Cancer Research UK Cambridge Institute

text: public

text: E-MTAB-5522:SLX-11312.N702_S508.H5H5YBBXX.s_8.r_1

text: ArrayExpress

text: embryonic stem cell

text: house mouse

text: Mus musculus

characteristic: organism

text: Mus musculus

text: induced CBFB-MYH11 oncogene expression

text: Mus musculus

text: OK


text: Cancer Research UK Cambridge Institute

characteristic: INSDC center name

text: public

text: E-MTAB-5522:SLX-11312.N702_S517.H5H5YBBXX.s_8.r_1

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: embryonic stem cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: wild type phenotype

characteristic: phenotype

text: Mus musculus

text: OK


text: Cancer Research UK Cambridge Institute

text: public

text: E-MTAB-5522:SLX-11312.N703_S502.H5H5YBBXX.s_8.r_1

text: ArrayExpress

text: embryonic stem cell

characteristic: cell type

text: house mouse

characteristic: common name

text: Protocols: The murine multipotent myeloid progenitor cell line 416B was stably transduced with a TetOn construct of the Cbfb-Myh11 (CM) oncogene (type A cDNA), using an in-frame F2A-mCherry protein as a reporter. As a control, cells were alternatively transduced with a version of the construct lacking the CM cDNA. 416B cells were maintained in RPMI medium, supplemented with 10% fetal calf serum and antibiotics. Expression of the CM oncogene or mCherry was induced by treatment with 1 ug/ml of doxycycline, and induction was confirmed after 24 hours by measurement of mCherry levels by fluorescence activated cell sorting. Reverse transcription was performed in a final volume of 13.2 ul per well, containing 1 uM of oligo-dT (Sigma-Aldrich), 1.04 mM of each dNTP (ThermoFisher), 100 U of SuperScript II retrotranscriptase (Invitrogen/ThermoFisher), 5 U of RNase inhibitor (Ambion), 5 mM of DTT, 1 M of Betaine (Sigma-Alrich), 6 mM of MgCl$_2$ (Ambion) and 1 uM of TSO primer (Exiqon). Spike-in RNA was added such that each well contained a final volume of 0.1 ul of a 1:3,000,000 dilution of the ERCC RNA Spike-In Mix (Invitrogen/ThermoFisher) and 0.12 ul of a 1:3,000,000 dilution of the Spike-in RNA Variant (SIRV) Control Mix E0 (Lexogen). Cells were sorted into individual wells of a 96-well microtiter plate. Each well contained 2.3 ul of lysis buffer with RNAse inhibitor (Ambion) in a 0.2% (v/v) Triton X-100 solution. Preamplification was performed in a total volume of 27 ul that contained 13.5 ul of HiFi Hotstart ReadyMix (2x; KAPA Biosystems) and 0.1 uM of IS PCR primer (Sigma-Aldrich). After 23 cycles of amplification, samples were cleaned with 80% (v/v) of Ampure beads (Beckman Coulter). Sequencing libraries were prepared using the Nextera XT DNA sample preparation kit (Illumina).

characteristic: description

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: wild type phenotype

text: Mus musculus

text: OK

characteristic: single cell well quality


text: Cancer Research UK Cambridge Institute

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-5522:SLX-11312.N703_S503.H5H5YBBXX.s_8.r_1

text: ArrayExpress

text: embryonic stem cell

text: house mouse

text: Doxycycline-inducible CBFB-MYH11 oncogene

text: Mus musculus

text: Mus musculus

text: wild type phenotype

text: Mus musculus

text: OK

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Cancer Research UK Cambridge Institute

text: public

text: E-MTAB-5522:SLX-11312.N703_S504.H5H5YBBXX.s_8.r_1

text: ArrayExpress

text: embryonic stem cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: wild type phenotype

text: Mus musculus

text: OK

characteristic: strain


text: Cancer Research UK Cambridge Institute

characteristic: INSDC center name

text: public

text: E-MTAB-5522:SLX-11312.N703_S505.H5H5YBBXX.s_8.r_1

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: embryonic stem cell

text: house mouse

text: Mus musculus

characteristic: organism

text: Mus musculus

text: induced CBFB-MYH11 oncogene expression

text: Mus musculus

text: OK


text: Cancer Research UK Cambridge Institute

text: public

text: E-MTAB-5522:SLX-11312.N703_S506.H5H5YBBXX.s_8.r_1

text: ArrayExpress

text: embryonic stem cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: induced CBFB-MYH11 oncogene expression

characteristic: phenotype

text: Mus musculus

text: OK


text: Cancer Research UK Cambridge Institute

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-5522:SLX-11312.N703_S507.H5H5YBBXX.s_8.r_1

text: ArrayExpress

text: embryonic stem cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: induced CBFB-MYH11 oncogene expression

text: Mus musculus

text: OK

characteristic: single cell well quality


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Cancer Research UK Cambridge Institute

text: public

text: E-MTAB-5522:SLX-11312.N703_S508.H5H5YBBXX.s_8.r_1

text: ArrayExpress

text: embryonic stem cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: induced CBFB-MYH11 oncogene expression

text: Mus musculus

text: OK

characteristic: title


text: Cancer Research UK Cambridge Institute

characteristic: INSDC center name

text: public

text: E-MTAB-5522:SLX-11312.N703_S517.H5H5YBBXX.s_8.r_1

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: embryonic stem cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: wild type phenotype

text: Mus musculus

text: OK

characteristic: strain


text: Cancer Research UK Cambridge Institute

text: public

text: E-MTAB-5522:SLX-11312.N704_S502.H5H5YBBXX.s_8.r_1

text: ArrayExpress

text: embryonic stem cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

characteristic: genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: wild type phenotype

text: Mus musculus

text: OK


text: Cancer Research UK Cambridge Institute

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-5522:SLX-11312.N704_S503.H5H5YBBXX.s_8.r_1

text: ArrayExpress

text: embryonic stem cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: wild type phenotype

characteristic: phenotype

text: Mus musculus

text: OK


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Cancer Research UK Cambridge Institute

text: public

text: E-MTAB-5522:SLX-11312.N704_S504.H5H5YBBXX.s_8.r_1

text: ArrayExpress

text: embryonic stem cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: wild type phenotype

text: Mus musculus

text: OK

characteristic: single cell well quality


text: Cancer Research UK Cambridge Institute

characteristic: INSDC center name

text: public

text: E-MTAB-5522:SLX-11312.N704_S505.H5H5YBBXX.s_8.r_1

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: embryonic stem cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: induced CBFB-MYH11 oncogene expression

text: Mus musculus

text: OK

characteristic: title


text: Cancer Research UK Cambridge Institute

text: public

text: E-MTAB-5522:SLX-11312.N704_S506.H5H5YBBXX.s_8.r_1

text: ArrayExpress

text: embryonic stem cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: induced CBFB-MYH11 oncogene expression

text: Mus musculus

text: OK

characteristic: strain


text: Cancer Research UK Cambridge Institute

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-5522:SLX-11312.N704_S507.H5H5YBBXX.s_8.r_1

text: ArrayExpress

text: embryonic stem cell

text: house mouse

text: Mus musculus

characteristic: organism

text: Mus musculus

text: induced CBFB-MYH11 oncogene expression

text: Mus musculus

text: OK


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Cancer Research UK Cambridge Institute

text: public

text: E-MTAB-5522:SLX-11312.N704_S508.H5H5YBBXX.s_8.r_1

text: ArrayExpress

text: embryonic stem cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: induced CBFB-MYH11 oncogene expression

characteristic: phenotype

text: Mus musculus

text: OK


text: Cancer Research UK Cambridge Institute

characteristic: INSDC center name

text: public

text: E-MTAB-5522:SLX-11312.N704_S517.H5H5YBBXX.s_8.r_1

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: embryonic stem cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: wild type phenotype

text: Mus musculus

text: OK

characteristic: single cell well quality


text: Cancer Research UK Cambridge Institute

text: public

text: E-MTAB-5522:SLX-11312.N705_S502.H5H5YBBXX.s_8.r_1

text: ArrayExpress

text: embryonic stem cell

characteristic: cell type

text: house mouse

characteristic: common name

text: Protocols: The murine multipotent myeloid progenitor cell line 416B was stably transduced with a TetOn construct of the Cbfb-Myh11 (CM) oncogene (type A cDNA), using an in-frame F2A-mCherry protein as a reporter. As a control, cells were alternatively transduced with a version of the construct lacking the CM cDNA. 416B cells were maintained in RPMI medium, supplemented with 10% fetal calf serum and antibiotics. Expression of the CM oncogene or mCherry was induced by treatment with 1 ug/ml of doxycycline, and induction was confirmed after 24 hours by measurement of mCherry levels by fluorescence activated cell sorting. Reverse transcription was performed in a final volume of 13.2 ul per well, containing 1 uM of oligo-dT (Sigma-Aldrich), 1.04 mM of each dNTP (ThermoFisher), 100 U of SuperScript II retrotranscriptase (Invitrogen/ThermoFisher), 5 U of RNase inhibitor (Ambion), 5 mM of DTT, 1 M of Betaine (Sigma-Alrich), 6 mM of MgCl$_2$ (Ambion) and 1 uM of TSO primer (Exiqon). Spike-in RNA was added such that each well contained a final volume of 0.1 ul of a 1:3,000,000 dilution of the ERCC RNA Spike-In Mix (Invitrogen/ThermoFisher) and 0.12 ul of a 1:3,000,000 dilution of the Spike-in RNA Variant (SIRV) Control Mix E0 (Lexogen). Cells were sorted into individual wells of a 96-well microtiter plate. Each well contained 2.3 ul of lysis buffer with RNAse inhibitor (Ambion) in a 0.2% (v/v) Triton X-100 solution. Preamplification was performed in a total volume of 27 ul that contained 13.5 ul of HiFi Hotstart ReadyMix (2x; KAPA Biosystems) and 0.1 uM of IS PCR primer (Sigma-Aldrich). After 23 cycles of amplification, samples were cleaned with 80% (v/v) of Ampure beads (Beckman Coulter). Sequencing libraries were prepared using the Nextera XT DNA sample preparation kit (Illumina).

characteristic: description

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: wild type phenotype

text: Mus musculus

text: OK

characteristic: title


text: Cancer Research UK Cambridge Institute

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-5522:SLX-11312.N705_S503.H5H5YBBXX.s_8.r_1

text: ArrayExpress

text: embryonic stem cell

text: house mouse

text: Doxycycline-inducible CBFB-MYH11 oncogene

text: Mus musculus

text: Mus musculus

text: wild type phenotype

text: Mus musculus

text: OK

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Cancer Research UK Cambridge Institute

text: public

text: E-MTAB-5522:SLX-11312.N705_S504.H5H5YBBXX.s_8.r_1

text: ArrayExpress

text: embryonic stem cell

text: house mouse

text: Mus musculus

characteristic: organism

text: Mus musculus

text: wild type phenotype

text: Mus musculus

text: OK


text: Cancer Research UK Cambridge Institute

characteristic: INSDC center name

text: public

text: E-MTAB-5522:SLX-11312.N705_S505.H5H5YBBXX.s_8.r_1

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: embryonic stem cell

text: house mouse

text: Mus musculus

text: Mus musculus

text: induced CBFB-MYH11 oncogene expression

characteristic: phenotype

text: Mus musculus

text: OK


text: Cancer Research UK Cambridge Institute

text: public

text: E-MTAB-5522:SLX-11312.N705_S506.H5H5YBBXX.s_8.r_1

text: ArrayExpress

text: embryonic stem cell

characteristic: cell type

text: house mouse

characteristic: common name

characteristic: description

characteristic: genotype

text: Mus musculus

text: Mus musculus

text: induced CBFB-MYH11 oncogene expression

text: Mus musculus

text: OK

characteristic: single cell well quality