E-MTAB-5469:Sample 1

characteristic: External Id

text: Stazione Zoologica Anton Dohrn

text: public

text: E-MTAB-5469:Sample 1

tag: Namespace:Stazione Zoologica Anton Dohrn

text: ArrayExpress

characteristic: broker name

text: Protocols: To induce the sexual phase, synchronized cultures of MT+ and MT- mating type were co-cultured in the bipartite glass apparatus where MT+ and MT- strains were physically separated by a 0.22μm pore size membrane filter that allowed chemical communication between them.Simultaneously, MT+ and MT- strains were grown in separate glass flasks as controls. Two and 6 hours after the co-culture started, samples (200 mL each) for RNA extraction were collected from the two strains in co-culture and from the parental strains cultivated in monoculture. Samples were collected onto 1.2μm pore-size membrane filters (RAWP04700 Millipore), placed in TrizolTM, flash frozen in liquid nitrogen immediately and stored at -80 0C until RNA extractions. RNA was extracted following the manufacturer’s instructions (Trizol reagent, Invitrogen) and the genomic DNA contamination was removed by DNase I treatment (RNase-Free DNase Set Qiagen) followed by RNA purification using RNeasy Plant Mini Kit (Qiagen). The quantity of RNA was determined by Qubit assay (Qubit® 2.0 Fluorometer, Life Technologies) and integrity was assessed by Bioanalyzer (2100 Bioanalyzer Instruments, Agilent Technologies) as well as by running samples on a 1.5% agarose gel. Libraries were prepared using a Beckman Biomek FX and the Illumina® TruSeq® Stranded Total RNA Sample Preparation kit, with poly-A selection and starting with 500 ng total RNA

characteristic: description

text: monoclonal culture

characteristic: growth condition

text: MT+

characteristic: mating type

text: Pseudo-nitzschia multistriata

characteristic: organism

text: Pseudo-nitzschia multistriata

text: Pseudo-nitzschia multistriata

characteristic: strain

text: Sample 1


E-MTAB-5469:Sample 10

status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: Stazione Zoologica Anton Dohrn

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-5469:Sample 10

characteristic: Submitter Id

text: ArrayExpress

text: membrane separated co-culture with MT- strain (B939)

text: MT+

text: Pseudo-nitzschia multistriata

text: Pseudo-nitzschia multistriata

text: Pseudo-nitzschia multistriata

text: Sample 10

characteristic: title

type: has member


E-MTAB-5469:Sample 11

characteristic: External Id

text: Stazione Zoologica Anton Dohrn

text: public

text: E-MTAB-5469:Sample 11

text: ArrayExpress

text: monoclonal culture

text: MT-

text: Pseudo-nitzschia multistriata

text: Pseudo-nitzschia multistriata

text: Pseudo-nitzschia multistriata

text: Sample 11


E-MTAB-5469:Sample 12

text: Stazione Zoologica Anton Dohrn

text: public

text: E-MTAB-5469:Sample 12

text: ArrayExpress

characteristic: broker name

text: membrane separated co-culture with MT- strain (B856)

text: MT-

text: Pseudo-nitzschia multistriata

text: Pseudo-nitzschia multistriata

text: Pseudo-nitzschia multistriata

text: Sample 12


E-MTAB-5469:Sample 13

text: Stazione Zoologica Anton Dohrn

text: public

text: E-MTAB-5469:Sample 13

text: ArrayExpress

characteristic: description

text: monoclonal culture

text: MT+

text: Pseudo-nitzschia multistriata

text: Pseudo-nitzschia multistriata

text: Pseudo-nitzschia multistriata

text: Sample 13


E-MTAB-5469:Sample 14

text: Stazione Zoologica Anton Dohrn

text: public

text: E-MTAB-5469:Sample 14

text: ArrayExpress

characteristic: growth condition

text: MT+

text: Pseudo-nitzschia multistriata

text: Pseudo-nitzschia multistriata

text: Pseudo-nitzschia multistriata

text: Sample 14


E-MTAB-5469:Sample 15

text: Stazione Zoologica Anton Dohrn

text: public

text: E-MTAB-5469:Sample 15

text: ArrayExpress

text: monoclonal culture

text: MT-

characteristic: mating type

text: Pseudo-nitzschia multistriata

text: Pseudo-nitzschia multistriata

text: Pseudo-nitzschia multistriata

text: Sample 15


E-MTAB-5469:Sample 16

text: Stazione Zoologica Anton Dohrn

text: public

text: E-MTAB-5469:Sample 16

text: ArrayExpress

text: MT-

text: Pseudo-nitzschia multistriata

characteristic: organism

text: Pseudo-nitzschia multistriata

text: Pseudo-nitzschia multistriata

text: Sample 16


E-MTAB-5469:Sample 2

text: Stazione Zoologica Anton Dohrn

text: public

text: E-MTAB-5469:Sample 2

text: ArrayExpress

text: membrane separated co-culture with MT- strain (B857)

text: MT+

text: Pseudo-nitzschia multistriata

text: Pseudo-nitzschia multistriata

text: Pseudo-nitzschia multistriata

characteristic: strain

text: Sample 2


E-MTAB-5469:Sample 3

characteristic: ENA-CHECKLIST

text: Stazione Zoologica Anton Dohrn

text: public

text: E-MTAB-5469:Sample 3

text: ArrayExpress

text: monoclonal culture

text: MT-

text: Pseudo-nitzschia multistriata

text: Pseudo-nitzschia multistriata

text: Pseudo-nitzschia multistriata

text: Sample 3


E-MTAB-5469:Sample 4

characteristic: ENA-FIRST-PUBLIC

text: Stazione Zoologica Anton Dohrn

text: public

text: E-MTAB-5469:Sample 4

text: ArrayExpress

text: membrane separated co-culture with MT+ strain (B938)

text: MT-

text: Pseudo-nitzschia multistriata

text: Pseudo-nitzschia multistriata

text: Pseudo-nitzschia multistriata

text: Sample 4


E-MTAB-5469:Sample 5

characteristic: ENA-LAST-UPDATE

text: Stazione Zoologica Anton Dohrn

text: public

text: E-MTAB-5469:Sample 5

text: ArrayExpress

text: monoclonal culture

text: MT+

text: Pseudo-nitzschia multistriata

text: Pseudo-nitzschia multistriata

text: Pseudo-nitzschia multistriata

text: Sample 5


E-MTAB-5469:Sample 6

text: Stazione Zoologica Anton Dohrn

characteristic: INSDC center name

text: public

text: E-MTAB-5469:Sample 6

text: ArrayExpress

text: MT+

text: Pseudo-nitzschia multistriata

text: Pseudo-nitzschia multistriata

text: Pseudo-nitzschia multistriata

text: Sample 6


E-MTAB-5469:Sample 7

text: Stazione Zoologica Anton Dohrn

characteristic: INSDC first public

text: public

text: E-MTAB-5469:Sample 7

text: ArrayExpress

text: monoclonal culture

text: MT-

text: Pseudo-nitzschia multistriata

text: Pseudo-nitzschia multistriata

text: Pseudo-nitzschia multistriata

text: Sample 7


E-MTAB-5469:Sample 8

text: Stazione Zoologica Anton Dohrn

characteristic: INSDC last update

text: public

text: E-MTAB-5469:Sample 8

text: ArrayExpress

text: MT-

text: Pseudo-nitzschia multistriata

text: Pseudo-nitzschia multistriata

text: Pseudo-nitzschia multistriata

text: Sample 8


E-MTAB-5469:Sample 9

text: Stazione Zoologica Anton Dohrn

text: public

characteristic: INSDC status

text: E-MTAB-5469:Sample 9

text: ArrayExpress

text: monoclonal culture

text: MT+

text: Pseudo-nitzschia multistriata

text: Pseudo-nitzschia multistriata

text: Pseudo-nitzschia multistriata

text: Sample 9


GumanB

text: SEOUL NAT'L UNIV.

text: public

characteristic: SRA accession

text: GumanB

tag: Namespace:SEOUL NAT'L UNIV.

characteristic: collection date

text: Freshwater

characteristic: environment (biome)

text: River

characteristic: environment (feature)

text: Water

characteristic: environment (material)

text: South Korea

characteristic: geographic location (country and/or sea)

unit: m

characteristic: geographic location (depth)

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: uncultured virus

text: Han River Virome

characteristic: project name

text: uncultured virus

text: Illumina MiSeq

characteristic: sequencing method

text: Han River Virome

text: water

characteristic: water environmental package


Daesung

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SEOUL NAT'L UNIV.

characteristic: INSDC center name

text: public

text: Daesung

characteristic: Submitter Id

text: Freshwater

text: River

text: Water

text: South Korea

text: metagenome

text: uncultured virus

characteristic: organism

text: Han River Virome

text: uncultured virus

text: Illumina MiSeq

text: Han River Virome

characteristic: title

text: water


PaldangB

text: SEOUL NAT'L UNIV.

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: PaldangB

characteristic: collection date

text: Freshwater

characteristic: environment (biome)

text: River

characteristic: environment (feature)

text: Water

characteristic: environment (material)

text: South Korea

text: metagenome

text: uncultured virus

text: Han River Virome

text: uncultured virus

text: Illumina MiSeq

text: Han River Virome

text: water


HannamB

text: SEOUL NAT'L UNIV.

text: public

text: HannamB

text: Freshwater

text: River

text: Water

text: South Korea

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: uncultured virus

text: Han River Virome

characteristic: project name

text: uncultured virus

text: Illumina MiSeq

characteristic: sequencing method

text: Han River Virome

text: water

characteristic: water environmental package


IlsanB

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SEOUL NAT'L UNIV.

characteristic: INSDC center name

text: public

text: IlsanB

characteristic: Submitter Id

text: Freshwater

text: River

text: Water

text: South Korea

text: metagenome

text: uncultured virus

characteristic: organism

text: Han River Virome

text: uncultured virus

text: Illumina MiSeq

text: Han River Virome

characteristic: title

text: water


text: male

characteristic: ArrayExpress-Sex

text: Oryzias latipes

characteristic: ArrayExpress-Species

text: Inbred

characteristic: ArrayExpress-StrainOrLine

text: SC

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Japanese medaka

characteristic: common name

text: Oryzias latipes

text: Inbred Medaka fish

characteristic: sample description

text: Oryzias latipes

text: Inbred

characteristic: strain


text: Oryzias latipes

text: Inbred

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: Japanese medaka

text: Oryzias latipes

text: Inbred Medaka fish

text: Oryzias latipes

text: Inbred


L00798 K11/14-02

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Friedrich-Loeffler-Institut

text: public

text: L00798 K11/14-02

text: not provided

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: Porcine epidemic diarrhea virus

text: Porcine epidemic diarrhea in Europe: In-detail analyses of disease dynamics and virus evolution

characteristic: description

text: Germany

characteristic: geographic location (country and/or sea)

text: Baden-Wuerttemberg

characteristic: geographic location (region and locality)

text: domestic

characteristic: host behaviour

text: swine

characteristic: host common name

text: domestic:indoor farm

characteristic: host habitat

text: diseased

characteristic: host health state

text: Sus scrofa

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

characteristic: isolate

text: feces

characteristic: isolation source host associated

text: Porcine epidemic diarrhea virus

text: farm sample

characteristic: sample capture status

text: Porcine epidemic diarrhea virus

text: Porcine epidemic diarrhea in Europe

characteristic: virus identifier


L00799 K11/14-01

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Friedrich-Loeffler-Institut

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: L00799 K11/14-01

characteristic: Submitter Id

text: not provided

characteristic: collecting institution

text: not provided

text: Porcine epidemic diarrhea virus

characteristic: common name

text: Germany

text: Baden-Wuerttemberg

text: domestic

text: swine

text: domestic:indoor farm

text: diseased

text: Sus scrofa

text: not provided

text: not provided

text: feces

text: Porcine epidemic diarrhea virus

characteristic: organism

text: farm sample

text: Porcine epidemic diarrhea virus

text: Porcine epidemic diarrhea in Europe

characteristic: title


L00855 K14/14-02

text: Friedrich-Loeffler-Institut

text: public

text: L00855 K14/14-02

text: not provided

characteristic: collection date

text: not provided

characteristic: collector name

text: Porcine epidemic diarrhea virus

characteristic: description

text: Germany

characteristic: geographic location (country and/or sea)

text: Baden-Wuerttemberg

characteristic: geographic location (region and locality)

text: domestic

characteristic: host behaviour

text: swine

characteristic: host common name

text: domestic:indoor farm

characteristic: host habitat

text: diseased

characteristic: host health state

text: Sus scrofa

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

characteristic: isolate

text: feces

characteristic: isolation source host associated

text: Porcine epidemic diarrhea virus

text: farm sample

text: Porcine epidemic diarrhea virus

text: Porcine epidemic diarrhea in Europe


L00857 K14/14-04

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Friedrich-Loeffler-Institut

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: L00857 K14/14-04

characteristic: Submitter Id

text: not provided

characteristic: collecting institution

text: not provided

text: Porcine epidemic diarrhea virus

characteristic: common name

text: Germany

text: Baden-Wuerttemberg

text: domestic

text: swine

text: domestic:indoor farm

text: diseased

text: Sus scrofa

text: not provided

text: not provided

text: feces

text: Porcine epidemic diarrhea virus

text: farm sample

characteristic: sample capture status

text: Porcine epidemic diarrhea virus

text: Porcine epidemic diarrhea in Europe

characteristic: virus identifier


text: Friedrich-Loeffler-Institut

text: public

text: not provided

characteristic: collection date

text: not provided

characteristic: collector name

text: Porcine epidemic diarrhea virus

characteristic: description

text: Germany

characteristic: geographic location (country and/or sea)

text: domestic

characteristic: host behaviour

text: swine

characteristic: host common name

text: domestic:indoor farm

characteristic: host habitat

text: diseased

characteristic: host health state

text: Sus scrofa

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: feces

text: Porcine epidemic diarrhea virus

characteristic: organism

text: farm sample

text: Porcine epidemic diarrhea virus

text: Porcine epidemic diarrhea in Europe

characteristic: title


L00901 V215/78

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Friedrich-Loeffler-Institut

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: L00901 V215/78

characteristic: Submitter Id

text: not provided

characteristic: collecting institution

text: not provided

text: Porcine epidemic diarrhea virus

text: Germany

text: domestic

text: swine

text: domestic:indoor farm

text: diseased

text: Sus scrofa

text: not provided

text: not provided

characteristic: isolate

text: not provided

characteristic: isolation source host associated

text: Porcine epidemic diarrhea virus

text: farm sample

text: Porcine epidemic diarrhea virus

text: Porcine epidemic diarrhea in Europe


L00906 K16/14-01

text: Friedrich-Loeffler-Institut

text: public

text: L00906 K16/14-01

text: not provided

characteristic: collection date

text: not provided

characteristic: collector name

text: Porcine epidemic diarrhea virus

characteristic: common name

characteristic: description

text: Germany

characteristic: geographic location (country and/or sea)

text: Baden-Wuerttemberg

characteristic: geographic location (region and locality)

text: domestic

characteristic: host behaviour

text: swine

characteristic: host common name

text: domestic:indoor farm

characteristic: host habitat

text: diseased

characteristic: host health state

text: Sus scrofa

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

text: feces

text: Porcine epidemic diarrhea virus

text: farm sample

characteristic: sample capture status

text: Porcine epidemic diarrhea virus

text: Porcine epidemic diarrhea in Europe

characteristic: virus identifier


L00907 K16/14-02

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Friedrich-Loeffler-Institut

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: L00907 K16/14-02

characteristic: Submitter Id

text: not provided

characteristic: collecting institution

text: not provided

text: Porcine epidemic diarrhea virus

text: Germany

text: Baden-Wuerttemberg

text: domestic

text: swine

text: domestic:indoor farm

text: diseased

text: Sus scrofa

text: not provided

text: not provided

characteristic: host subject id

text: feces

text: Porcine epidemic diarrhea virus

characteristic: organism

text: farm sample

text: Porcine epidemic diarrhea virus

text: Porcine epidemic diarrhea in Europe

characteristic: title


L00908 K16/14-03

text: Friedrich-Loeffler-Institut

text: public

text: L00908 K16/14-03

text: not provided

characteristic: collection date

text: not provided

characteristic: collector name

text: Porcine epidemic diarrhea virus

characteristic: common name

characteristic: description

text: Germany

characteristic: geographic location (country and/or sea)

text: Baden-Wuerttemberg

characteristic: geographic location (region and locality)

text: domestic

characteristic: host behaviour

text: swine

characteristic: host common name

text: domestic:indoor farm

characteristic: host habitat

text: diseased

characteristic: host health state

text: Sus scrofa

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: isolate

text: feces

characteristic: isolation source host associated

text: Porcine epidemic diarrhea virus

text: farm sample

text: Porcine epidemic diarrhea virus

text: Porcine epidemic diarrhea in Europe


L00918 K17/14-01

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Friedrich-Loeffler-Institut

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: L00918 K17/14-01

characteristic: Submitter Id

text: not provided

characteristic: collecting institution

text: not provided

text: Porcine epidemic diarrhea virus

text: Germany

text: domestic

text: swine

text: domestic:indoor farm

text: diseased

text: Sus scrofa

text: not provided

text: not provided

characteristic: host subject id

text: feces

text: Porcine epidemic diarrhea virus

text: farm sample

characteristic: sample capture status

text: Porcine epidemic diarrhea virus

text: Porcine epidemic diarrhea in Europe

characteristic: virus identifier


L00919 K17/14-02

text: Friedrich-Loeffler-Institut

text: public

text: L00919 K17/14-02

text: not provided

characteristic: collection date

text: not provided

characteristic: collector name

text: Porcine epidemic diarrhea virus

characteristic: common name

characteristic: description

text: Germany

characteristic: geographic location (country and/or sea)

text: domestic

characteristic: host behaviour

text: swine

characteristic: host common name

text: domestic:indoor farm

characteristic: host habitat

text: diseased

characteristic: host health state

text: Sus scrofa

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

text: feces

text: Porcine epidemic diarrhea virus

characteristic: organism

text: farm sample

text: Porcine epidemic diarrhea virus

text: Porcine epidemic diarrhea in Europe

characteristic: title


L00926 K20/14-01

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Friedrich-Loeffler-Institut

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: L00926 K20/14-01

characteristic: Submitter Id

text: not provided

characteristic: collecting institution

text: not provided

text: Porcine epidemic diarrhea virus

text: Germany

text: Bayern

characteristic: geographic location (region and locality)

text: domestic

text: swine

text: domestic:indoor farm

text: diseased

text: Sus scrofa

text: not provided

text: not provided

characteristic: isolate

text: feces

characteristic: isolation source host associated

text: Porcine epidemic diarrhea virus

text: farm sample

text: Porcine epidemic diarrhea virus

text: Porcine epidemic diarrhea in Europe


L00927 K20/14-02

text: Friedrich-Loeffler-Institut

text: public

text: L00927 K20/14-02

text: not provided

characteristic: collection date

text: not provided

characteristic: collector name

text: Porcine epidemic diarrhea virus

characteristic: common name

characteristic: description

text: Germany

characteristic: geographic location (country and/or sea)

text: Baden-Wurttemberg

text: domestic

characteristic: host behaviour

text: swine

characteristic: host common name

text: domestic:indoor farm

characteristic: host habitat

text: diseased

characteristic: host health state

text: Sus scrofa

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: feces

text: Porcine epidemic diarrhea virus

text: farm sample

characteristic: sample capture status

text: Porcine epidemic diarrhea virus

text: Porcine epidemic diarrhea in Europe

characteristic: virus identifier


L00928 K20/14-03

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Friedrich-Loeffler-Institut

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: L00928 K20/14-03

characteristic: Submitter Id

text: not provided

characteristic: collecting institution

text: not provided

text: Porcine epidemic diarrhea virus

text: Germany

text: Baden-Wuerttemberg

characteristic: geographic location (region and locality)

text: domestic

text: swine

text: domestic:indoor farm

text: diseased

text: Sus scrofa

text: not provided

text: not provided

text: feces

text: Porcine epidemic diarrhea virus

characteristic: organism

text: farm sample

text: Porcine epidemic diarrhea virus

text: Porcine epidemic diarrhea in Europe

characteristic: title


L00931 K22/14-03

text: Friedrich-Loeffler-Institut

text: public

text: L00931 K22/14-03

text: not provided

characteristic: collection date

text: not provided

characteristic: collector name

text: Porcine epidemic diarrhea virus

characteristic: common name

characteristic: description

text: Germany

characteristic: geographic location (country and/or sea)

text: Baden-Wuerttemberg

text: domestic

characteristic: host behaviour

text: swine

characteristic: host common name

text: domestic:indoor farm

characteristic: host habitat

text: diseased

characteristic: host health state

text: Sus scrofa

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

characteristic: isolate

text: feces

characteristic: isolation source host associated

text: Porcine epidemic diarrhea virus

text: farm sample

text: Porcine epidemic diarrhea virus

text: Porcine epidemic diarrhea in Europe


L00932 K22/14-04

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Friedrich-Loeffler-Institut

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: L00932 K22/14-04

characteristic: Submitter Id

text: not provided

characteristic: collecting institution

text: not provided

text: Porcine epidemic diarrhea virus

text: Germany

text: Baden-Wuerttemberg

characteristic: geographic location (region and locality)

text: domestic

text: swine

text: domestic:indoor farm

text: diseased

text: Sus scrofa

text: not provided

text: not provided

text: feces

text: Porcine epidemic diarrhea virus

text: farm sample

characteristic: sample capture status

text: Porcine epidemic diarrhea virus

text: Porcine epidemic diarrhea in Europe

characteristic: virus identifier


L00933 K22/14-05

text: Friedrich-Loeffler-Institut

text: public

text: L00933 K22/14-05

text: not provided

characteristic: collection date

text: not provided

characteristic: collector name

text: Porcine epidemic diarrhea virus

characteristic: common name

characteristic: description

text: Germany

characteristic: geographic location (country and/or sea)

text: Baden-Wuerttemberg

text: domestic

characteristic: host behaviour

text: swine

characteristic: host common name

text: domestic:indoor farm

characteristic: host habitat

text: diseased

characteristic: host health state

text: Sus scrofa

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: feces

text: Porcine epidemic diarrhea virus

characteristic: organism

text: farm sample

text: Porcine epidemic diarrhea virus

text: Porcine epidemic diarrhea in Europe

characteristic: title


L00998 K06/15-03

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Friedrich-Loeffler-Institut

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: L00998 K06/15-03

characteristic: Submitter Id

text: not provided

characteristic: collecting institution

text: not provided

text: Porcine epidemic diarrhea virus

text: Germany

text: Lower Saxony

characteristic: geographic location (region and locality)

text: domestic

text: swine

text: domestic:indoor farm

text: diseased

text: Sus scrofa

text: not provided

text: not provided

characteristic: isolate

text: feces

characteristic: isolation source host associated

text: Porcine epidemic diarrhea virus

text: farm sample

text: Porcine epidemic diarrhea virus

text: Porcine epidemic diarrhea in Europe


L00999 K06/15-07

text: Friedrich-Loeffler-Institut

text: public

text: L00999 K06/15-07

text: not provided

characteristic: collection date

text: not provided

characteristic: collector name

text: Porcine epidemic diarrhea virus

characteristic: common name

characteristic: description

text: Germany

characteristic: geographic location (country and/or sea)

text: Schleswig Holstein

text: domestic

characteristic: host behaviour

text: swine

characteristic: host common name

text: domestic:indoor farm

characteristic: host habitat

text: diseased

characteristic: host health state

text: Sus scrofa

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: feces

text: Porcine epidemic diarrhea virus

text: farm sample

characteristic: sample capture status

text: Porcine epidemic diarrhea virus

text: Porcine epidemic diarrhea in Europe

characteristic: virus identifier


L01011 K01/15-01

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Friedrich-Loeffler-Institut

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: L01011 K01/15-01

characteristic: Submitter Id

text: not provided

characteristic: collecting institution

text: not provided

text: Porcine epidemic diarrhea virus

text: Germany

text: Lower Saxony

characteristic: geographic location (region and locality)

text: domestic

text: swine

text: domestic:indoor farm

text: diseased

text: Sus scrofa

text: not provided

text: not provided

text: feces

text: Porcine epidemic diarrhea virus

characteristic: organism

text: farm sample

text: Porcine epidemic diarrhea virus

text: Porcine epidemic diarrhea in Europe

characteristic: title


L01012 K01/15-02

text: Friedrich-Loeffler-Institut

text: public

text: L01012 K01/15-02

text: not provided

characteristic: collection date

text: not provided

characteristic: collector name

text: Porcine epidemic diarrhea virus

characteristic: common name

characteristic: description

text: Germany

characteristic: geographic location (country and/or sea)

text: Lower Saxony

text: domestic

characteristic: host behaviour

text: swine

characteristic: host common name

text: domestic:indoor farm

characteristic: host habitat

text: diseased

characteristic: host health state

text: Sus scrofa

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

characteristic: isolate

text: feces

characteristic: isolation source host associated

text: Porcine epidemic diarrhea virus

text: farm sample

text: Porcine epidemic diarrhea virus

text: Porcine epidemic diarrhea in Europe


L01013 K01/15-03

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Friedrich-Loeffler-Institut

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: L01013 K01/15-03

characteristic: Submitter Id

text: not provided

characteristic: collecting institution

text: not provided

text: Porcine epidemic diarrhea virus

text: Germany

text: North Rhine-Westphalia

characteristic: geographic location (region and locality)

text: domestic

text: swine

text: domestic:indoor farm

text: diseased

text: Sus scrofa

text: not provided

text: not provided

text: feces

text: Porcine epidemic diarrhea virus

text: farm sample

characteristic: sample capture status

text: Porcine epidemic diarrhea virus

text: Porcine epidemic diarrhea in Europe

characteristic: virus identifier


L01014 K01/15-04

text: Friedrich-Loeffler-Institut

text: public

text: L01014 K01/15-04

text: not provided

characteristic: collection date

text: not provided

characteristic: collector name

text: Porcine epidemic diarrhea virus

characteristic: common name

characteristic: description

text: Germany

characteristic: geographic location (country and/or sea)

text: North Rhine-Westphalia

text: domestic

characteristic: host behaviour

text: swine

characteristic: host common name

text: domestic:indoor farm

characteristic: host habitat

text: diseased

characteristic: host health state

text: Sus scrofa

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: feces

text: Porcine epidemic diarrhea virus

characteristic: organism

text: farm sample

text: Porcine epidemic diarrhea virus

text: Porcine epidemic diarrhea in Europe

characteristic: title


L01015 K01/15-05

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Friedrich-Loeffler-Institut

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: L01015 K01/15-05

characteristic: Submitter Id

text: not provided

characteristic: collecting institution

text: not provided

text: Porcine epidemic diarrhea virus

text: Germany

text: Lower Saxony

characteristic: geographic location (region and locality)

text: domestic

text: swine

text: domestic:indoor farm

text: diseased

text: Sus scrofa

text: not provided

text: not provided

characteristic: isolate

text: feces

characteristic: isolation source host associated

text: Porcine epidemic diarrhea virus

text: farm sample

text: Porcine epidemic diarrhea virus

text: Porcine epidemic diarrhea in Europe


L01017 K01/15-07

text: Friedrich-Loeffler-Institut

text: public

text: L01017 K01/15-07

text: not provided

characteristic: collection date

text: not provided

characteristic: collector name

text: Porcine epidemic diarrhea virus

characteristic: common name

characteristic: description

text: Germany

characteristic: geographic location (country and/or sea)

text: Bavaria

text: domestic

characteristic: host behaviour

text: swine

characteristic: host common name

text: domestic:indoor farm

characteristic: host habitat

text: diseased

characteristic: host health state

text: Sus scrofa

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: feces

text: Porcine epidemic diarrhea virus

text: farm sample

characteristic: sample capture status

text: Porcine epidemic diarrhea virus

text: Porcine epidemic diarrhea in Europe

characteristic: virus identifier


L01018 K01/15-08

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Friedrich-Loeffler-Institut

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: L01018 K01/15-08

characteristic: Submitter Id

text: not provided

characteristic: collecting institution

text: not provided

text: Porcine epidemic diarrhea virus

text: Germany

text: Bavaria

characteristic: geographic location (region and locality)

text: domestic

text: swine

text: domestic:indoor farm

text: diseased

text: Sus scrofa

text: not provided

text: not provided

text: feces

text: Porcine epidemic diarrhea virus

characteristic: organism

text: farm sample

text: Porcine epidemic diarrhea virus

text: Porcine epidemic diarrhea in Europe

characteristic: title


L01019 K01/15-09

text: Friedrich-Loeffler-Institut

text: public

text: L01019 K01/15-09

text: not provided

characteristic: collection date

text: not provided

characteristic: collector name

text: Porcine epidemic diarrhea virus

characteristic: common name

characteristic: description

text: Germany

characteristic: geographic location (country and/or sea)

text: Bavaria

text: domestic

characteristic: host behaviour

text: swine

characteristic: host common name

text: domestic:indoor farm

characteristic: host habitat

text: diseased

characteristic: host health state

text: Sus scrofa

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

characteristic: isolate

text: feces

characteristic: isolation source host associated

text: Porcine epidemic diarrhea virus

text: farm sample

text: Porcine epidemic diarrhea virus

text: Porcine epidemic diarrhea in Europe


L01020 K01/15-10

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Friedrich-Loeffler-Institut

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: L01020 K01/15-10

characteristic: Submitter Id

text: not provided

characteristic: collecting institution

text: not provided

text: Porcine epidemic diarrhea virus

text: Germany

text: Thuringia

characteristic: geographic location (region and locality)

text: domestic

text: swine

text: domestic:indoor farm

text: diseased

text: Sus scrofa

text: not provided

text: not provided

text: feces

text: Porcine epidemic diarrhea virus

text: farm sample

characteristic: sample capture status

text: Porcine epidemic diarrhea virus

text: Porcine epidemic diarrhea in Europe

characteristic: virus identifier


L01059 K07/15-01

text: Friedrich-Loeffler-Institut

text: public

text: L01059 K07/15-01

text: not provided

characteristic: collection date

text: not provided

characteristic: collector name

text: Porcine epidemic diarrhea virus

characteristic: common name

characteristic: description

text: Germany

characteristic: geographic location (country and/or sea)

text: Thuringia

text: domestic

characteristic: host behaviour

text: swine

characteristic: host common name

text: domestic:indoor farm

characteristic: host habitat

text: diseased

characteristic: host health state

text: Sus scrofa

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: feces

text: Porcine epidemic diarrhea virus

characteristic: organism

text: farm sample

text: Porcine epidemic diarrhea virus

text: Porcine epidemic diarrhea in Europe

characteristic: title


L01060 K07/15-02

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Friedrich-Loeffler-Institut

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: L01060 K07/15-02

characteristic: Submitter Id

text: not provided

characteristic: collecting institution

text: not provided

text: Porcine epidemic diarrhea virus

text: Germany

text: North Rhine-Westphalia

characteristic: geographic location (region and locality)

text: domestic

text: swine

text: domestic:indoor farm

text: diseased

text: Sus scrofa

text: not provided

text: not provided

characteristic: isolate

text: feces

characteristic: isolation source host associated

text: Porcine epidemic diarrhea virus

text: farm sample

text: Porcine epidemic diarrhea virus

text: Porcine epidemic diarrhea in Europe


L01061 K07/15-03

text: Friedrich-Loeffler-Institut

text: public

text: L01061 K07/15-03

text: not provided

characteristic: collection date

text: not provided

characteristic: collector name

text: Porcine epidemic diarrhea virus

characteristic: common name

characteristic: description

text: Germany

characteristic: geographic location (country and/or sea)

text: Thuringia

text: domestic

characteristic: host behaviour

text: swine

characteristic: host common name

text: domestic:indoor farm

characteristic: host habitat

text: diseased

characteristic: host health state

text: Sus scrofa

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: feces

text: Porcine epidemic diarrhea virus

text: farm sample

characteristic: sample capture status

text: Porcine epidemic diarrhea virus

text: Porcine epidemic diarrhea in Europe

characteristic: virus identifier


L01062 M10/15-01

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Friedrich-Loeffler-Institut

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: L01062 M10/15-01

characteristic: Submitter Id

text: not provided

characteristic: collecting institution

text: not provided

text: Porcine epidemic diarrhea virus

text: Austria

text: domestic

text: swine

text: domestic:indoor farm

text: diseased

text: Sus scrofa

text: not provided

text: not provided

text: feces

text: Porcine epidemic diarrhea virus

characteristic: organism

text: farm sample

text: Porcine epidemic diarrhea virus

text: Porcine epidemic diarrhea in Europe

characteristic: title


L01063 M10/15-02

text: Friedrich-Loeffler-Institut

text: public

text: L01063 M10/15-02

text: not provided

characteristic: collection date

text: not provided

characteristic: collector name

text: Porcine epidemic diarrhea virus

characteristic: common name

characteristic: description

text: Austria

characteristic: geographic location (country and/or sea)

text: domestic

characteristic: host behaviour

text: swine

characteristic: host common name

text: domestic:indoor farm

characteristic: host habitat

text: diseased

characteristic: host health state

text: Sus scrofa

characteristic: host scientific name

text: not provided

characteristic: host sex

text: not provided

characteristic: isolate

text: feces

characteristic: isolation source host associated

text: Porcine epidemic diarrhea virus

text: farm sample

text: Porcine epidemic diarrhea virus

text: Porcine epidemic diarrhea in Europe


L01064 M10/15-03

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Friedrich-Loeffler-Institut

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: L01064 M10/15-03

characteristic: Submitter Id

text: not provided

text: not provided

text: Porcine epidemic diarrhea virus

text: Austria

text: domestic

text: swine

text: domestic:indoor farm

text: diseased

text: Sus scrofa

text: not provided

text: not provided

characteristic: host subject id

text: feces

text: Porcine epidemic diarrhea virus

text: farm sample

characteristic: sample capture status

text: Porcine epidemic diarrhea virus

text: Porcine epidemic diarrhea in Europe

characteristic: virus identifier


L01065 M10/15-04

text: Friedrich-Loeffler-Institut

text: public

text: L01065 M10/15-04

text: not provided

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: Porcine epidemic diarrhea virus

characteristic: common name

characteristic: description

text: Austria

characteristic: geographic location (country and/or sea)

text: domestic

characteristic: host behaviour

text: swine

characteristic: host common name

text: domestic:indoor farm

characteristic: host habitat

text: diseased

characteristic: host health state

text: Sus scrofa

characteristic: host scientific name

text: not provided

text: not provided

text: feces

text: Porcine epidemic diarrhea virus

characteristic: organism

text: farm sample

text: Porcine epidemic diarrhea virus

text: Porcine epidemic diarrhea in Europe

characteristic: title


L01329 K25/15-01

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Friedrich-Loeffler-Institut

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: L01329 K25/15-01

characteristic: Submitter Id

text: not provided

text: not provided

text: Porcine epidemic diarrhea virus

text: Romania

text: domestic

text: swine

text: domestic:indoor farm

text: diseased

text: Sus scrofa

text: not provided

characteristic: host sex

text: not provided

characteristic: isolate

text: feces

characteristic: isolation source host associated

text: Porcine epidemic diarrhea virus

text: farm sample

text: Porcine epidemic diarrhea virus

text: Porcine epidemic diarrhea in Europe


L01330 K25/15-02

text: Friedrich-Loeffler-Institut

text: public

text: L01330 K25/15-02

text: not provided

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: Porcine epidemic diarrhea virus

characteristic: common name

characteristic: description

text: Romania

characteristic: geographic location (country and/or sea)

text: domestic

characteristic: host behaviour

text: swine

characteristic: host common name

text: domestic:indoor farm

characteristic: host habitat

text: diseased

characteristic: host health state

text: Sus scrofa

text: not provided

text: not provided

characteristic: host subject id

text: feces

text: Porcine epidemic diarrhea virus

text: farm sample

characteristic: sample capture status

text: Porcine epidemic diarrhea virus

text: Porcine epidemic diarrhea in Europe

characteristic: virus identifier


L01420 K06/15-04

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Friedrich-Loeffler-Institut

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: L01420 K06/15-04

characteristic: Submitter Id

text: not provided

text: not provided

text: Porcine epidemic diarrhea virus

text: Germany

text: North Rhine-Westphalia

characteristic: geographic location (region and locality)

text: domestic

text: swine

text: domestic:indoor farm

text: diseased

text: Sus scrofa

characteristic: host scientific name

text: not provided

text: not provided

text: feces

text: Porcine epidemic diarrhea virus

characteristic: organism

text: farm sample

text: Porcine epidemic diarrhea virus

text: Porcine epidemic diarrhea in Europe

characteristic: title


L00926 K20/14-01 T

text: Friedrich-Loeffler-Institut

text: public

text: L00926 K20/14-01 T

text: not provided

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: Porcine torovirus

characteristic: common name

characteristic: description

text: Germany

characteristic: geographic location (country and/or sea)

text: Bayern

text: domestic

characteristic: host behaviour

text: swine

characteristic: host common name

text: domestic:indoor farm

characteristic: host habitat

text: diseased

characteristic: host health state

text: Sus scrofa

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

characteristic: isolate

text: feces

characteristic: isolation source host associated

text: Porcine torovirus

text: farm sample

text: Porcine torovirus

text: Porcine epidemic diarrhea in Europe


L00928 K20/14-03 E

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Friedrich-Loeffler-Institut

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: L00928 K20/14-03 E

characteristic: Submitter Id

text: not provided

text: not provided

text: Enterobacteria Phage MS2

text: Germany

text: Baden-Wuerttemberg

characteristic: geographic location (region and locality)

text: domestic

text: swine

text: domestic:indoor farm

text: diseased

text: Sus scrofa

characteristic: host scientific name

text: not provided

text: not provided

text: feces

text: Escherichia virus MS2

text: farm sample

characteristic: sample capture status

text: Escherichia virus MS2

text: Porcine epidemic diarrhea in Europe

characteristic: virus identifier


L00798 K11/14-02 S

text: Friedrich-Loeffler-Institut

text: public

text: L00798 K11/14-02 S

text: not provided

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: Porcine sapelovirus A

characteristic: common name

characteristic: description

text: Germany

characteristic: geographic location (country and/or sea)

text: Baden-Wuerttemberg

text: domestic

characteristic: host behaviour

text: swine

characteristic: host common name

text: domestic:indoor farm

characteristic: host habitat

text: diseased

characteristic: host health state

text: Sus scrofa

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: feces

text: Sapelovirus A

characteristic: organism

text: farm sample

text: Sapelovirus A

text: Porcine epidemic diarrhea in Europe

characteristic: title


L01017 K01/15-07 Po

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Friedrich-Loeffler-Institut

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: L01017 K01/15-07 Po

characteristic: Submitter Id

text: not provided

text: not provided

text: Posavirus 1

text: Germany

text: Bavaria

characteristic: geographic location (region and locality)

text: domestic

text: swine

text: domestic:indoor farm

text: diseased

text: Sus scrofa

characteristic: host scientific name

text: not provided

text: not provided

characteristic: isolate

text: feces

characteristic: isolation source host associated

text: Posavirus 1

text: farm sample

text: Posavirus 1

text: Porcine epidemic diarrhea in Europe


L00919 K17/14-02 K

text: Friedrich-Loeffler-Institut

text: public

text: L00919 K17/14-02 K

text: not provided

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: Porcine kobuvirus

characteristic: common name

characteristic: description

text: Germany

characteristic: geographic location (country and/or sea)

text: domestic

characteristic: host behaviour

text: swine

characteristic: host common name

text: domestic:indoor farm

characteristic: host habitat

text: diseased

characteristic: host health state

text: Sus scrofa

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: feces

text: Porcine kobuvirus

text: farm sample

characteristic: sample capture status

text: Porcine kobuvirus

text: Porcine epidemic diarrhea in Europe

characteristic: virus identifier


L00855 K14/14-02 A

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Friedrich-Loeffler-Institut

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: L00855 K14/14-02 A

characteristic: Submitter Id

text: not provided

text: not provided

text: Porcine astrovirus

text: Germany

text: Baden-Wuerttemberg

characteristic: geographic location (region and locality)

text: domestic

text: swine

text: domestic:indoor farm

text: diseased

text: Sus scrofa

characteristic: host scientific name

text: not provided

text: not provided

text: feces

text: Mamastrovirus 3

characteristic: organism

text: farm sample

text: Mamastrovirus 3

text: Porcine epidemic diarrhea in Europe

characteristic: title


L00919 K17/14-02 A

text: Friedrich-Loeffler-Institut

text: public

text: L00919 K17/14-02 A

text: not provided

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: Porcine astrovirus

characteristic: common name

characteristic: description

text: Germany

characteristic: geographic location (country and/or sea)

text: domestic

characteristic: host behaviour

text: swine

characteristic: host common name

text: domestic:indoor farm

characteristic: host habitat

text: diseased

characteristic: host health state

text: Sus scrofa

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

characteristic: isolate

text: feces

characteristic: isolation source host associated

text: Mamastrovirus 3

text: farm sample

text: Mamastrovirus 3

text: Porcine epidemic diarrhea in Europe


L00721 P

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Friedrich-Loeffler-Institut

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: L00721 P

characteristic: Submitter Id

text: not provided

text: not provided

text: Pasivirus A

text: Germany

text: Baden-Wuerttemberg

characteristic: geographic location (region and locality)

text: domestic

text: swine

text: domestic:indoor farm

text: diseased

text: Sus scrofa

characteristic: host scientific name

text: not provided

text: not provided

text: feces

text: Pasivirus A

text: farm sample

characteristic: sample capture status

text: Pasivirus A

text: Porcine epidemic diarrhea in Europe

characteristic: virus identifier


L00799 K11/14-01 Pa

text: Friedrich-Loeffler-Institut

text: public

text: L00799 K11/14-01 Pa

text: not provided

characteristic: collecting institution

characteristic: collection date

text: not provided

characteristic: collector name

text: Pasivirus A

characteristic: common name

characteristic: description

text: Germany

characteristic: geographic location (country and/or sea)

text: Baden-Wuerttemberg

text: domestic

characteristic: host behaviour

text: swine

characteristic: host common name

text: domestic:indoor farm

characteristic: host habitat

text: diseased

characteristic: host health state

text: Sus scrofa

text: not provided

characteristic: host sex

text: not provided

characteristic: host subject id

text: feces

text: Pasivirus A

characteristic: organism

text: farm sample

text: Pasivirus A

text: Porcine epidemic diarrhea in Europe

characteristic: title


L01061 K07/15-03 Pa

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: Friedrich-Loeffler-Institut

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: L01061 K07/15-03 Pa

characteristic: Submitter Id

text: not provided

text: not provided

text: Pasivirus A

text: Germany

text: Thuringia

characteristic: geographic location (region and locality)

text: domestic

text: swine

text: domestic:indoor farm

text: diseased

text: Sus scrofa

characteristic: host scientific name

text: not provided

text: not provided

characteristic: isolate

text: feces

characteristic: isolation source host associated

text: Pasivirus A

text: farm sample

text: Pasivirus A

text: Porcine epidemic diarrhea in Europe


text: WUR

text: public

text: Experimental mixture containing 1% Zea mays, 1% Glycine max, 1% Aloe variegata, 1% Dendrobium sp., 1% Huso Dauricus, 1% Crocodylus niloticus, 47% Brassica oleracea and 47% Bos taurus, in dry weight percentages.

characteristic: description

text: mixed sample

text: mixed sample

text: Interlaboratory trial


text: WUR

text: public

text: Experimental mixture containing 1% Zea mays, 1% Gossypium hirsutum, 2% Aloe variegata, 2% Dendrobium sp., 2% Huso Dauricus, 2% Crocodylus niloticus, 45% Brassica oleracea and 45% Bos taurus, in dry weight percentages.

text: mixed sample

characteristic: organism

text: mixed sample

text: Interlaboratory trial


text: ISTITUTO SUPERIORE DI SANITA

text: public

tag: Namespace:ISTITUTO SUPERIORE DI SANITA

characteristic: collection date

text: Romania

characteristic: geographic location (country and/or sea)

text: human

characteristic: host

text: diseased

characteristic: host health state

text: Homo sapiens

text: faeces

characteristic: isolation source

text: Escherichia coli

text: Escherichia coli

characteristic: serovar

characteristic: strain

text: VTEC

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: WUR

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Traditional medicine with the following ingredients on the label: Herba Cistanches, Cauda cervi, Radix Rehmanniae praeparata, Radix Ginseng, Radix morindae Officinalis, Semen Cuscutae, Radix Achyranthis bidentatae, Rhizoma Cibotii, Semen Platycladi, Cortex Eucommiae, Radix Astragali, Fructus Schisandrae chinensis, Cortex Cinnamomi and Cornu Cervi Pantotrichum

text: mixed sample

text: mixed sample

text: Interlaboratory trial


text: WUR

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: Experimental mixture containing 1% Zea mays, 1% Brassica napus, 3% Aloe variegata, 3% Dendrobium sp., 3% Huso Dauricus, 3% Crocodylus niloticus, 43% Brassica oleracea and 43% Bos taurus, in dry weight percentages.

characteristic: description

text: mixed sample

characteristic: organism

text: mixed sample

text: Interlaboratory trial


text: WUR

text: public

text: Experimental mixture containing 1% Zea mays, 1% Triticum aestivum, 4% Aloe variegata, 4% Dendrobium sp., 4% Huso Dauricus, 4% Crocodylus niloticus, 41% Brassica oleracea and 41% Bos taurus, in dry weight percentages.

text: mixed sample

text: mixed sample

text: Interlaboratory trial


text: WUR

text: public

text: Experimental mixture containing 1% Zea mays, 1% Beta vulgaris, 1% Aloe variegata, 1% Dendrobium sp., 1% Huso Dauricus, 1% Crocodylus niloticus, 10% Lactuca sativa, 32% Brassica oleracea, 10% Sus scrofa, 32% Bos taurus and 10% pleuronectus platessa, in dry weight percentages.

text: mixed sample

text: mixed sample

text: Interlaboratory trial


text: WUR

text: public

text: Experimental mixture containing 1% Zea mays, 1% Meleagris gallopavo, 2% Aloe variegata, 2% Dendrobium sp., 2% Huso Dauricus, 2% Crocodylus niloticus, 10% Lactuca sativa, 30% Brassica oleracea, 10% Sus scrofa, 30% Bos taurus and 10% pleuronectus platessa, in dry weight percentages.

text: mixed sample

text: mixed sample

text: Interlaboratory trial


text: WUR

text: public

text: Traditional medicine with the following ingredients on the label: Kola nut, Siberian ginseng, horny goat weed, Catuaba, Muria puama, Korean ginseng, Damiana, Saw palmetto, Yohimbe and Magnesium stearate

text: mixed sample

text: mixed sample

text: Interlaboratory trial


text: Gut Metagenome

characteristic: ArrayExpress-Phenotype

text: Gut Metagenome

characteristic: ArrayExpress-Species

text: SC

text: public

text: Gut Metagenome

characteristic: common name

text: gut metagenome

text: Mouse Faecal

characteristic: sample description

text: gut metagenome


text: PROIMI

text: public

characteristic: collection date

text: Diamante Lake is a multi-extreme environment located in Catamarca province, Argentina. Developing on the bottom of calcareous rocks we found Red Biofilms (DLRB).

text: small lake biome

characteristic: environment (biome)

text: alkaline salt lake

characteristic: environment (feature)

text: biofilm material

characteristic: environment (material)

text: Argentina

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: microbial mat/biofilm

characteristic: microbial mat/biofilm environmental package

text: biofilm metagenome

text: Diamante Lake Red Biofilm WGS

characteristic: project name

text: biofilm metagenome

text: Pyrosequencing

characteristic: sequencing method

text: Diamante Lake Red Biofilms

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

text: months

characteristic: age units

characteristic: anonymized name

text: K

characteristic: description

text: TRUE

characteristic: elevation

text: urban biome

characteristic: env biome

text: animal-associated habitat

characteristic: env feature

text: host-associated

characteristic: env package

text: organic material

text: USA:CA:Davis

characteristic: geo loc name

text: pig

characteristic: host common name

text: Sus scrofa

characteristic: host scientific name

text: K

characteristic: host subject id

characteristic: host taxid

characteristic: latitude

characteristic: longitude

text: pig gut metagenome

characteristic: organism

text: UCDavis

text: TRUE

text: cecal sample

characteristic: sample type

text: pig gut metagenome

text: Effect of dietary lignan on pig cecal microbiome


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

text: months

text: K

text: TRUE

text: urban biome

text: animal-associated habitat

text: host-associated

text: organic material

text: USA:CA:Davis

text: pig

text: Sus scrofa

text: K

text: pig gut metagenome

text: UCDavis

text: TRUE

text: cecal sample

text: pig gut metagenome

characteristic: title


text: UCSDMI

text: public

text: months

characteristic: age units

characteristic: anonymized name

text: LR

characteristic: description

text: TRUE

characteristic: elevation

text: urban biome

characteristic: env biome

text: animal-associated habitat

characteristic: env feature

text: host-associated

characteristic: env package

text: organic material

text: USA:CA:Davis

characteristic: geo loc name

text: pig

characteristic: host common name

text: Sus scrofa

characteristic: host scientific name

text: LR

characteristic: host subject id

characteristic: host taxid

text: pig gut metagenome

text: UCDavis

text: TRUE

text: cecal sample

text: pig gut metagenome


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: months

text: LRA

text: TRUE

text: urban biome

text: animal-associated habitat

text: host-associated

text: organic material

text: USA:CA:Davis

text: pig

text: Sus scrofa

text: LRA

characteristic: latitude

characteristic: longitude

text: pig gut metagenome

characteristic: organism

text: UCDavis

text: TRUE

text: cecal sample

characteristic: sample type

text: pig gut metagenome


text: UCSDMI

text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: age

text: months

characteristic: age units

characteristic: anonymized name

text: LRA

characteristic: description

text: TRUE

characteristic: elevation

text: urban biome

characteristic: env biome

text: animal-associated habitat

characteristic: env feature

text: host-associated

characteristic: env package

text: organic material

text: USA:CA:Davis

characteristic: geo loc name

text: pig

text: Sus scrofa

text: LRA

text: pig gut metagenome

text: UCDavis

text: TRUE

text: cecal sample

text: pig gut metagenome

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: months

text: LR

text: TRUE

text: urban biome

text: animal-associated habitat

text: host-associated

text: organic material

text: USA:CA:Davis

text: pig

characteristic: host common name

text: Sus scrofa

characteristic: host scientific name

text: LR

characteristic: host subject id

characteristic: host taxid

text: pig gut metagenome

text: UCDavis

text: TRUE

text: cecal sample

text: pig gut metagenome


Socompa

text: PROIMI

text: public

characteristic: SRA accession

text: Socompa

characteristic: Submitter Id

characteristic: collection date

text: Socompa Lake stromatolite homogenate for WGS sequencing

characteristic: description

text: Xeric basin biome

characteristic: environment (biome)

text: saline lake

characteristic: environment (feature)

text: microbial mat material

characteristic: environment (material)

text: Argentina

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: microbial mat/biofilm

characteristic: microbial mat/biofilm environmental package

text: stromatolite metagenome

characteristic: organism

characteristic: ph

text: Microbial communities in Andean South America Altiplane

characteristic: project name

text: stromatolite metagenome

text: Illumina

characteristic: sequencing method

text: Socompa lake stromatolite homogenate


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: PROIMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Microbial mat from La Brava lake

text: Xeric basin biome

text: saline pan

text: saline lake sediment

text: Chile

text: metagenome

text: microbial mat/biofilm

text: microbial mat metagenome

text: microbial mat metagenome

text: Illumina

text: La Brava microbial mat

characteristic: title


text: PROIMI

text: public

characteristic: collection date

text: Microbial mat from Tebenquiche lake

characteristic: description

text: Xeric basin biome

characteristic: environment (biome)

text: saline pan

characteristic: environment (feature)

text: saline lake sediment

characteristic: environment (material)

text: Chile

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: microbial mat/biofilm

text: microbial mat metagenome

text: microbial mat metagenome

text: Illumina

text: Tebenquiche microbial mat


HaengjuB

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: SEOUL NAT'L UNIV.

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: HaengjuB

text: Water

text: River

text: Water

text: South Korea

text: metagenome

text: uncultured virus

characteristic: organism

text: Han River Virome

characteristic: project name

text: uncultured virus

text: Illumina MiSeq

characteristic: sequencing method

text: Han River Virome

text: water

characteristic: water environmental package


unit: mg Chl/m3

characteristic: Chlorophyll Sensor

characteristic: Event Date/Time End

text: INSTITUTE OF EVOLUTIONARY BIOLOGY

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: Latitude End

characteristic: Longitude End

characteristic: SRA accession

unit: practical salinity unit

characteristic: Salinity Sensor

tag: Namespace:INSTITUTE OF EVOLUTIONARY BIOLOGY

characteristic: Submitter Id

characteristic: depth

text: Arabian sea

characteristic: environment (biome)

text: Ocean

characteristic: environment (feature)

text: marine water

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

characteristic: latitude start

characteristic: longitude start

text: Arabian sea

characteristic: marine region

unit: µmol/L

characteristic: nitrate sensor

text: Monosiga brevicollis ATCC 50154

unit: µmol/kg

characteristic: oxygen sensor

text: Monosiga brevicollis single-cell amplified genomes

characteristic: protocol label

characteristic: sampling campaign

text: Research Vessel Tara

characteristic: sampling platform

characteristic: sampling station

text: Monosiga brevicollis ATCC 50154

unit: ºC

characteristic: temperature

text: MB4 SAG M.brevicollis

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University Hospital Galway

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:University Hospital Galway

characteristic: Submitter Id

characteristic: collection date

text: Ireland

characteristic: geographic location (country and/or sea)

text: Homo sapiens

characteristic: host scientific name

characteristic: isolate

text: Human

characteristic: isolation source

text: Salmonella enterica subsp. enterica serovar Typhimurium

characteristic: organism

text: Typhimurium

characteristic: serovar

text: Subsp. I

characteristic: sub species


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: University Hospital Galway

text: public

text: Ireland

text: Homo sapiens

text: Human

text: Salmonella enterica subsp. enterica serovar Java

text: Java

text: Subsp. I

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University Hospital Galway

characteristic: INSDC center name

text: public

text: Ireland

text: Homo sapiens

text: Human

text: Salmonella enterica subsp. enterica serovar Infantis

text: Infantis

text: Subsp. I


text: University Hospital Galway

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: Ireland

text: Homo sapiens

text: Human

text: Salmonella enterica subsp. enterica serovar Newport

text: Newport

text: Subsp. I


text: University Hospital Galway

text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: Ireland

text: Homo sapiens

text: Human

text: Salmonella enterica subsp. enterica serovar Bovismorbificans

text: Bovismorbificans

text: Subsp. I


text: University Hospital Galway

text: public

text: Ireland

characteristic: geographic location (country and/or sea)

text: Homo sapiens

characteristic: host scientific name

characteristic: isolate

text: Human

text: Salmonella enterica subsp. enterica serovar Rissen

text: Rissen

text: Subsp. I


text: University Hospital Galway

text: public

text: Ireland

text: Homo sapiens

text: Human

characteristic: isolation source

text: Salmonella enterica subsp. enterica serovar Enteritidis

characteristic: organism

text: Enteritidis

characteristic: serovar

text: Subsp. I


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: University Hospital Galway

text: public

text: Ireland

text: Homo sapiens

text: Human

text: Salmonella enterica subsp. enterica serovar Derby

text: Derby

text: Subsp. I

characteristic: sub species


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: WUR

text: public

text: mixed sample

text: mixed sample

text: Interlaboratory trial

characteristic: title


characteristic: Chlorophyll Sensor

characteristic: Event Date/Time End

text: INSTITUTE OF EVOLUTIONARY BIOLOGY

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: Latitude End

characteristic: Longitude End

characteristic: Salinity Sensor

characteristic: depth

text: Atolls of the Maldives

characteristic: environment (biome)

text: Open sea water

characteristic: environment (feature)

text: marine water

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

characteristic: latitude start

characteristic: longitude start

text: Atolls of the Maldives

characteristic: marine region

characteristic: nitrate sensor

text: Monosiga brevicollis ATCC 50154

characteristic: oxygen sensor

text: Single-cell amplified genomes of Monosiga brevicollis

characteristic: project name

characteristic: protocol label

characteristic: sampling campaign

text: Research Vessel Tara

characteristic: sampling platform

characteristic: sampling station

text: Monosiga brevicollis ATCC 50154

characteristic: temperature

text: MB2 SAG M.brevicollis


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: WUR

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Experimental mixture containing 1% Zea mays, 1% Solanum lycopersicum, 4% Aloe variegata, 4% Dendrobium sp., 4% Huso Dauricus, 4% Crocodylus niloticus, 10% Lactuca sativa, 26% Brassica oleracea, 10% Sus scrofa, 26% Bos taurus and 10% pleuronectus platessa, in dry weight percentages.

characteristic: description

text: mixed sample

characteristic: organism

text: mixed sample

text: Sample 10 Interlaboratory trial

characteristic: title


text: WUR

text: public

text: mixed sample

text: mixed sample

text: Interlaboratory trial


characteristic: Chlorophyll Sensor

characteristic: Event Date/Time End

text: INSTITUTE OF EVOLUTIONARY BIOLOGY

text: public

characteristic: Latitude End

characteristic: Longitude End

characteristic: Salinity Sensor

characteristic: depth

text: Arabian sea

characteristic: environment (biome)

text: Ocean open waters

characteristic: environment (feature)

text: sea water

characteristic: environment (material)

text: water

characteristic: environmental package

characteristic: event date/time start

characteristic: latitude start

characteristic: longitude start

text: Arabian sea

characteristic: marine region

characteristic: nitrate sensor

text: Monosiga brevicollis ATCC 50154

characteristic: oxygen sensor

text: M.brevicollis single-cell amplified genomes

characteristic: project name

characteristic: protocol label

characteristic: sampling campaign

text: Research Vessel Tara

text: Monosiga brevicollis ATCC 50154

text: MB1 SAG M.brevicollis


E-MTAB-5477:Sample 1

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-5477:Sample 1

tag: Namespace:CNRS-UMR7622, Laboratoire de Biologie du Developpement Institut de Biologie Paris-Seine

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: epithelial cell

characteristic: cell type

text: human

characteristic: common name

text: Protocols: P-bodies (cytosolic RNP granules) were purified as follow. GFP-LSM14A and GFP-LSM14A HEK293 cells were grown to 80% confluence in DMEM supplemented with 10% fetal calf serum. Starting from two plates of 15 mm for each cell line, cells were washed twice in PBS and scrapped in 2mL PBS. Cell pellets were frozen and stored at -80°C. Except stated otherwise, all the following steps were either conducted at 4°C or on ice. Pellets were suspended in lysis buffer (50 mM Tris (pH 7.4), 1 mM EDTA, 150 mM NaCl, 0,2% Triton X-100), in the presence of 65 U/mL RNaseOut recombinant ribonuclease inhibitor (Promega) and protease inhibitor cocktail without EDTA (Roche Diagnostics, Meylan, France). To accelerate cell lysis, cell extracts were passed 20-times through a syringe needle (27Gx3/4”, Nr. 20, 0.4X20mm, RW/LB, Henke Sass Wolf), for a total incubation time in lysis buffer of 20 min. Cells were spun for 5 minutes at 200xg to deplete nuclei. To eliminate remaining DNA contaminants, supernatants were supplemented with MgSO4 and CaCl2 (10 mM and 1 mM final, respectively) and were treated with 4 U/mL of RQ1 DNAse (Promega) for 30 min at RT. Extracts were further spun for 7 min at 10000xg, and pellets were re-suspended into 40 L of lysis buffer with 80 Units of RNaseOut recombinant ribonuclease inhibitor (Promega). This organelle enriched 10000xg pellet fraction was named the pre-sorted fraction. Next, P-bodies were sorted on a cell sorter (MoFlo Astrios EQ, Beckman Coulter) equipped with a nozzle size of 70 m, and working at 66 000Hz with a 30psi pressure. Particles were detected according to their Forward-scattered light (FSC) and through their green fluorescence using the 488nm excitation laser and the 526/52 band pass filter. To define the P-body sorting window, we compared an extract in which P-bodies were fluorescently labelled (GFP-LSM14A) to an extract in which P-bodies were not fluorescent (GFP-LSM14A). The purity mode (1-2 envelop) and a differential pressure of 0.5 were applied for sorting. Note that the starting dilution of the pre-sorted extract was critical for the purity of sorted P-bodies. To this end, a dilution of the presorted extract (10 l in 2 mL lysis buffer) was adjusted to detect 10000 Event Per Second (EPS). P-Bodies represented 10% of all detected EPS, and 1000 P-bodies were sorted per second for a sorting efficiency of 85%. After 6 hours of sorting, we cumulated 21E6 sorted particles. The collected fraction was spun for 7 min at 10000xg to pellet the sorted P-bodies. The sorted P-bodies and matching pre-sorted fractions were stored at minus 80°C. To fluorescently labels P-bodies, Human embryonic kidney HEK293 were stably transfected with a GFP-LSM14A plasmid. Briefly, HEK293 cells were maintained in DMEM supplemented with 10% fetal calf serum. Plasmid transfections were performed with 1 µg DNA per 35 mm diameter dish using GenJet Plus DNA (SignaGen Laboratories, Rockville, USA ) according to the manufacturer's protocol. The GFP-LSM14A plasmid was generated by cloning in frame the human LSM14A isoform1 (NCBI Reference Sequence: NM-001114093.1) ORF fragment (1392 bp) into pEGFP-C3 vector (BamHI/EcoR1 restriction sites), using In-Fusion HD cloning kit (Takara Bio, USA). For stable transfection, HEK293 cells were transfected with GFP-LSM14A plasmid as described above. 48 h later, cells were split in 100 mm diameter dishes and selected in the presence of 50 μg/ml geneticin sulfate (Invitrogen, France). After 3 days of selection, cells were further split to clonal dilution in 96-well plates. Clones of interest were selected using fluorescence microscopy to control for GFP-LSM14A expression levels and subcellular localization. Following Trizol LS (Invitrogen) extraction, the P-body sorted fractions gave a yield of ~30 ng of RNAs. Starting from 3 biological replicates, cDNAs were synthetized using random priming for the sorted P-bodies and pre-sorted fraction RNA libraries (Ovation RNA-Seq system NuGEN)

characteristic: description

text: embryo

characteristic: developmental stage

characteristic: genotype

characteristic: growth condition

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: kidney

characteristic: organism part

text: Homo sapiens

text: Sample 1

characteristic: title


E-MTAB-5477:Sample 2

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: CNRS-UMR7622, Laboratoire de Biologie du Developpement Institut de Biologie Paris-Seine

characteristic: INSDC center name

text: public

text: E-MTAB-5477:Sample 2

text: ArrayExpress

text: epithelial cell

text: human

text: embryo

text: DMEM supplemented with 10% fetal calf serum

text: Homo sapiens

text: Homo sapiens

text: kidney

text: Homo sapiens

text: Sample 2


E-MTAB-5477:Sample 3

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-5477:Sample 3

characteristic: Submitter Id

text: ArrayExpress

text: epithelial cell

text: human

text: embryo

text: Homo sapiens

text: Homo sapiens

text: kidney

text: Homo sapiens

text: Sample 3


E-MTAB-5477:Sample 4

text: public

text: E-MTAB-5477:Sample 4

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: epithelial cell

characteristic: cell type

text: human

characteristic: common name

characteristic: description

text: embryo

text: Homo sapiens

text: Homo sapiens

text: kidney

text: Homo sapiens

text: Sample 4


E-MTAB-5477:Sample 5

text: public

text: E-MTAB-5477:Sample 5

text: ArrayExpress

text: epithelial cell

text: human

text: embryo

characteristic: developmental stage

characteristic: genotype

characteristic: growth condition

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: kidney

characteristic: organism part

text: Homo sapiens

text: Sample 5


E-MTAB-5477:Sample 6

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-5477:Sample 6

text: ArrayExpress

text: epithelial cell

text: human

text: embryo

text: Homo sapiens

text: Homo sapiens

text: kidney

text: Homo sapiens

text: Sample 6

characteristic: title


text: EMBL-EBI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

unit: month

characteristic: age

text: ArrayExpress

text: naive thymus-derived CD4-positive, alpha-beta T cell

text: western European house mouse

text: Protocols: Cells were purified from spleen in two different sub-species of mice (C57BL/6 and CAST/EiJ; all male animals) using EASY cell strainer (30 μm, Greiner BioOne), cell separation media (lympholyte, #CL5035) and the CD4+ CD62L+ T Cell Isolation Kit II (Miltenyi Biotec, #130-093-227). We use a microfluidics-based approach (5-10 μm Auto Prep Integrated Fluidic Circuit - IFC, Fluidigm, San Francisco, CA) to capture single cells and the C1 Single cell Auto Prep System (Fluidigm) to prepare single-cell RNA-Seq libraries CD4+ T cells. Purified cells were directly loaded into the microfluidics (naive CD4+ T cells) or were stimulated on 96 round-plates coated with anti-CD3ε and anti-CD28 for 3 hours (activated CD4+ T cells). Naive and effector memory (EM) CD4+ T cells were purified from spleens of both young and old C57/BL6 mice by FACS. Briefly, spleens were harvested from both young and old animals and single cell suspensions were obtained by meshing through a cell strainer (70 μm). B cells were depleted from cell suspensions by MACS using CD19 microbeads (Miltenyi Biotec, #130-052-201) and red blood cells were lysed with RBC lysis buffer (Biolegend, #B205551). The enriched cell fraction was then stained with Fixable eFluor 780 viability dye (eBioscience) following by Fc receptor blocking with TruStain fcXTM (clone: 39, Biolegend) and subsequent staining with a panel of fluorescence-conjugated antibodies against CD4 (clone: RM4-5, BioLegend), CD44 (clone: IM7, BioLegend), CD62L (clone: MEL-14, BioLegend), CD24 (clone: M1/69, BioLegend), Qa2 (clone: 695H1-9-9, BioLegend), CD69 (clone: H1.2F3, BioLegend) and PD-1 (clone: RMP1-30, BioLegend). Stained cells were immediately sorted using a 5-laser Aria IIu SORP instrument (BD Biosciences). Lymphocytes were gated by the use of forward scatter (FSC-A) and side scatter (SSC-A). Cell doublets were excluded according to area and height of forward scatter (FSC-A/FSC-H). Dead cells were removed using viability dye. PD-1+ CD4+ T cells were excluded and PD-1-ve CD4+ T cells were t further separated into naive and EM CD4+ T cell subsets according to their CD44 and CD62L expression. Cells with a mature CD24lo Qa2hi phenotype were then gated from naive and EM subsets and CD69+ cells were removed. Purified cells were directly loaded into the microfluidics (naive CD4+ T cells) or were stimulated on 96 round-plates coated with anti-CD3 and anti-CD28 for 3 hours (activated CD4+ T cells) cDNAs were made on-chip (Fluidigm C1, 5-10um IFC) with Clontech SMARTer Ultra Low RNA kit. ERCC spike-in RNA (Ambion) (1 µL diluted at 1:50,000) was added to the C1 lysis mix Sequencing libraries were prepared using Illumina Nextera XT kit (Illumina) using the recommendation in the Fluidigm protocol (# PN 100-7168 K1).

text: B6 young 3

characteristic: individual

text: Mus musculus domesticus

text: Mus musculus domesticus

text: Mus musculus domesticus

text: male

characteristic: sex

characteristic: single cell id

text: not OK

characteristic: single cell well quality

characteristic: strain


text: EMBL-EBI

text: public

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: western European house mouse

characteristic: common name

characteristic: description

text: B6 young 3

text: Mus musculus domesticus

characteristic: organism

text: Mus musculus domesticus

text: Mus musculus domesticus

text: male

text: OK


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMBL-EBI

characteristic: INSDC center name

text: public

text: ArrayExpress

text: western European house mouse

text: B6 young 3

text: Mus musculus domesticus

text: Mus musculus domesticus

text: Mus musculus domesticus

text: male

text: OK

text: anti-CD3e and anti-CD28 for 3 hours

characteristic: stimulus

characteristic: title


text: EMBL-EBI

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: age

text: ArrayExpress

text: western European house mouse

text: B6 young 3

characteristic: individual

text: Mus musculus domesticus

text: Mus musculus domesticus

text: Mus musculus domesticus

text: male

characteristic: sex

text: not OK

text: anti-CD3e and anti-CD28 for 3 hours


text: EMBL-EBI

text: public

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: western European house mouse

characteristic: common name

text: B6 young 4

text: Mus musculus domesticus

text: Mus musculus domesticus

text: Mus musculus domesticus

text: male

characteristic: single cell id

text: OK

characteristic: single cell well quality

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMBL-EBI

characteristic: INSDC center name

text: public

text: ArrayExpress

text: western European house mouse

characteristic: description

text: B6 young 4

text: Mus musculus domesticus

characteristic: organism

text: Mus musculus domesticus

text: Mus musculus domesticus

text: male

text: not OK


text: EMBL-EBI

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: age

text: ArrayExpress

text: western European house mouse

text: B6 young 4

text: Mus musculus domesticus

text: Mus musculus domesticus

text: Mus musculus domesticus

text: male

text: not OK

text: anti-CD3e and anti-CD28 for 3 hours

characteristic: stimulus

characteristic: title


text: EMBL-EBI

text: public

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: western European house mouse

characteristic: common name

text: B6 young 4

characteristic: individual

text: Mus musculus domesticus

text: Mus musculus domesticus

text: Mus musculus domesticus

text: male

characteristic: sex

characteristic: single cell id

text: OK

text: anti-CD3e and anti-CD28 for 3 hours


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMBL-EBI

characteristic: INSDC center name

text: public

text: ArrayExpress

text: western European house mouse

text: B6 old 3

text: Mus musculus domesticus

text: Mus musculus domesticus

text: Mus musculus domesticus

text: male

text: OK

characteristic: single cell well quality

characteristic: strain


text: EMBL-EBI

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: age

text: ArrayExpress

text: western European house mouse

characteristic: description

text: B6 old 3

text: Mus musculus domesticus

characteristic: organism

text: Mus musculus domesticus

text: Mus musculus domesticus

text: male

text: not OK


text: EMBL-EBI

text: public

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: western European house mouse

characteristic: common name

text: B6 old 3

characteristic: individual

text: Mus musculus domesticus

text: Mus musculus domesticus

text: Mus musculus domesticus

text: male

text: OK

text: anti-CD3e and anti-CD28 for 3 hours

characteristic: stimulus

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMBL-EBI

characteristic: INSDC center name

text: public

text: ArrayExpress

text: western European house mouse

text: B6 old 3

text: Mus musculus domesticus

text: Mus musculus domesticus

text: Mus musculus domesticus

text: male

characteristic: sex

characteristic: single cell id

text: not OK

text: anti-CD3e and anti-CD28 for 3 hours


text: EMBL-EBI

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: age

text: ArrayExpress

text: western European house mouse

text: B6 old 4

text: Mus musculus domesticus

text: Mus musculus domesticus

text: Mus musculus domesticus

text: male

text: not OK

characteristic: single cell well quality

characteristic: strain


text: EMBL-EBI

text: public

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: western European house mouse

characteristic: common name

characteristic: description

text: B6 old 4

characteristic: individual

text: Mus musculus domesticus

characteristic: organism

text: Mus musculus domesticus

text: Mus musculus domesticus

text: male

text: OK


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMBL-EBI

characteristic: INSDC center name

text: public

text: ArrayExpress

text: western European house mouse

text: B6 old 4

text: Mus musculus domesticus

text: Mus musculus domesticus

text: Mus musculus domesticus

text: male

text: not OK

text: anti-CD3e and anti-CD28 for 3 hours

characteristic: stimulus

characteristic: title


text: EMBL-EBI

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: age

text: ArrayExpress

text: western European house mouse

text: B6 old 4

text: Mus musculus domesticus

text: Mus musculus domesticus

text: Mus musculus domesticus

text: male

characteristic: sex

characteristic: single cell id

text: OK

text: anti-CD3e and anti-CD28 for 3 hours


text: EMBL-EBI

text: public

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: effector memory CD4-positive, alpha-beta T cell

characteristic: cell type

text: western European house mouse

characteristic: common name

characteristic: description

text: B6 young 5

text: Mus musculus domesticus

text: Mus musculus domesticus

text: Mus musculus domesticus

text: male

text: not OK

characteristic: single cell well quality

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMBL-EBI

characteristic: INSDC center name

text: public

text: ArrayExpress

text: western European house mouse

text: B6 young 5

characteristic: individual

text: Mus musculus domesticus

characteristic: organism

text: Mus musculus domesticus

text: Mus musculus domesticus

text: male

text: OK


text: EMBL-EBI

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: age

text: ArrayExpress

text: western European house mouse

text: B6 young 5

text: Mus musculus domesticus

text: Mus musculus domesticus

text: Mus musculus domesticus

text: male

text: not OK

text: anti-CD3e and anti-CD28 for 3 hours

characteristic: stimulus

characteristic: title


text: EMBL-EBI

text: public

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: western European house mouse

characteristic: common name

characteristic: description

text: B6 young 5

text: Mus musculus domesticus

text: Mus musculus domesticus

text: Mus musculus domesticus

text: male

characteristic: sex

characteristic: single cell id

text: OK

text: anti-CD3e and anti-CD28 for 3 hours


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMBL-EBI

characteristic: INSDC center name

text: public

text: ArrayExpress

text: western European house mouse

text: B6 young 6

text: Mus musculus domesticus

text: Mus musculus domesticus

text: Mus musculus domesticus

text: male

text: OK

characteristic: single cell well quality

characteristic: strain


text: EMBL-EBI

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: age

text: ArrayExpress

text: western European house mouse

text: B6 young 6

characteristic: individual

text: Mus musculus domesticus

characteristic: organism

text: Mus musculus domesticus

text: Mus musculus domesticus

text: male

text: not OK


text: EMBL-EBI

text: public

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: western European house mouse

characteristic: common name

characteristic: description

text: B6 young 6

text: Mus musculus domesticus

text: Mus musculus domesticus

text: Mus musculus domesticus

text: male

text: not OK

text: anti-CD3e and anti-CD28 for 3 hours

characteristic: stimulus

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMBL-EBI

characteristic: INSDC center name

text: public

text: ArrayExpress

text: western European house mouse

text: B6 young 6

text: Mus musculus domesticus

text: Mus musculus domesticus

text: Mus musculus domesticus

text: male

characteristic: sex

characteristic: single cell id

text: OK

text: anti-CD3e and anti-CD28 for 3 hours


text: EMBL-EBI

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: age

text: ArrayExpress

text: western European house mouse

text: B6 old 5

text: Mus musculus domesticus

text: Mus musculus domesticus

text: Mus musculus domesticus

text: male

text: OK

characteristic: single cell well quality

characteristic: strain


text: EMBL-EBI

text: public

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

characteristic: cell type

text: western European house mouse

characteristic: common name

characteristic: description

text: B6 old 5

characteristic: individual

text: Mus musculus domesticus

characteristic: organism

text: Mus musculus domesticus

text: Mus musculus domesticus

text: male

text: not OK


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: EMBL-EBI

characteristic: INSDC center name

text: public

text: ArrayExpress

text: western European house mouse

text: B6 old 5

text: Mus musculus domesticus

text: Mus musculus domesticus

text: Mus musculus domesticus

text: male

text: OK

text: anti-CD3e and anti-CD28 for 3 hours

characteristic: stimulus

characteristic: title


text: EMBL-EBI

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: age

text: ArrayExpress

text: western European house mouse

text: B6 old 5

text: Mus musculus domesticus

text: Mus musculus domesticus

text: Mus musculus domesticus

text: male

characteristic: sex

characteristic: single cell id

text: not OK

text: anti-CD3e and anti-CD28 for 3 hours