text: INRA-CMSE

text: public

text: terrestrial biome

text: anthropogenic geographic feature

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: Madagascar

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: CGCAGTACGA

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: soil metagenome

characteristic: pcr primers

characteristic: project name

characteristic: sample volume or weight for DNA extraction

text: soil metagenome

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: 18S rRNA gene


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

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text: This study was carried out in the Highlands of Madagascar, usually characterized as being above 900 m above sea level. Hillsides (locally called �tanety�) are dominated by ferrallitic soils subjected to tropical altitude climate with a hot rainy season from November to April and a cold dry season from May to October. In May 2014, soil samples from 41 plots were sampled under similar vegetation (herbaceous savannah mainly composed of the grass Aristida sp., Poaceae). Plots were located along 3 different climatic gradients (Fig. 1) allowing the discrimination of mean annual temperature and precipitation effects. Temperature and precipitation of the first climatic gradient ranged from 18.6 to 25.5�C and from 1359 and 1508mm. Along the second gradient, temperature and precipitation ranged from 17.7 to 26.8�C and from 1327 to 1817mm. Along the third gradient, temperature and precipitation ranged from 16.4 to 22�C and from 779 to 1491mm. Mean annual temperature and rainfall were extracted from the WORLDCLIM database (Hijmans et al., 2005), obtained from 1950 to 2000, on the basis of each geographic coordinates of sampling. At each plot, six soil cores, 0-10 cm depth, were sampled with a metallic cylinder. One of them was used to measure the bulk density and other physicochemical analyses. The other 5 were pooled, sieved at 2 mm, and coarse plant debris were removed. Composite samples were kept at 4�C prior to further fresh soil incubations and analyses. For ten plots along the three gradients sampling was triplicated in order to assess the soil intrinsic variability under similar climate parameters.

characteristic: description

text: terrestrial biome

characteristic: environment (biome)

text: anthropogenic geographic feature

text: soil

text: Madagascar

unit: m

unit: DD

text: metagenome

text: TGTACTACTC

text: DNA was extracted using GnS-GII protocol as described in Terrat et al , 2012

text: soil metagenome

characteristic: organism

text: FR1 (5-ANCCATTCAATCGGTANT-3) FF390 (5-CGATAACGAACGAGACCT-3)

unit: g

text: soil metagenome

text: soil

text: 18S rRNA gene

characteristic: target gene

characteristic: title


text: INRA-CMSE

text: public

text: terrestrial biome

text: anthropogenic geographic feature

characteristic: environment (feature)

text: soil

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text: Madagascar

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characteristic: geographic location (depth)

characteristic: geographic location (elevation)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: CAGTAGACGT

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: soil metagenome

characteristic: pcr primers

characteristic: project name

characteristic: sample volume or weight for DNA extraction

text: soil metagenome

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: 18S rRNA gene


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

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text: INRA-CMSE

text: public

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text: Madagascar

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text: metagenome

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text: CGACACTATC

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text: soil metagenome

characteristic: sequencing method

text: soil

text: 18S rRNA gene

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: INRA-CMSE

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characteristic: description

text: terrestrial biome

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text: anthropogenic geographic feature

text: soil

text: Madagascar

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text: CAGACGTCTG

text: soil metagenome

characteristic: organism

text: soil metagenome

text: soil

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text: 18S rRNA gene


text: INRA-CMSE

text: public

text: terrestrial biome

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characteristic: environment (feature)

text: soil

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text: Madagascar

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text: metagenome

characteristic: investigation type

text: CGTCTAGTAC

characteristic: multiplex identifiers

characteristic: nucleic acid extraction

text: soil metagenome

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text: soil metagenome

characteristic: sequencing method

text: soil

text: 18S rRNA gene

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characteristic: ENA-CHECKLIST

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text: INRA-CMSE

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text: terrestrial biome

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text: soil

text: Madagascar

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text: INRA-CMSE

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characteristic: environment (feature)

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characteristic: environment (material)

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text: metagenome

characteristic: investigation type

text: CGAGCTCATG

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characteristic: soil environmental package

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text: INRA-CMSE

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text: terrestrial biome

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text: Madagascar

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text: CGTATGCGAC

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text: soil metagenome

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text: 18S rRNA gene

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text: UNIVERSITY OF ABERDEEN

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tag: Namespace:UNIVERSITY OF ABERDEEN

text: Campylobacter jejuni

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text: SC

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text: Influenzavirus B

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text: Influenza B virus (B/Paris/1762/2008)


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characteristic: External Id

text: SC

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text: Influenzavirus B

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text: Influenzavirus B

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text: public

text: Influenzavirus B

text: Influenza B virus (B/England/393/2007)

text: Influenza B virus (B/England/393/2007)


text: Influenzavirus B

text: SC

characteristic: INSDC last update

text: public

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text: Influenzavirus B

text: Influenza B virus (B/Odessa/3886/2010)

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text: Influenzavirus B

text: SC

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: Influenzavirus B

text: Influenza B virus (B/Stockholm/12/2011)

text: Influenza B virus (B/Stockholm/12/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Bretagne/1083/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Paris/1403/2011)

text: Influenza B virus (B/Paris/1403/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/England/243/2011)

text: Influenza B virus (B/England/243/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Iceland/21/2011)

text: Influenza B virus (B/Iceland/21/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Iceland/62/2011)

text: Influenza B virus (B/Iceland/62/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Paris/1260/2011)

text: Influenza B virus (B/Paris/1260/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Madagascar/1191/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Madagascar/2550/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Vilnius/703/2011)

text: Influenza B virus (B/Vilnius/703/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Astrakhan/22/2011)

text: Influenza B virus (B/Astrakhan/22/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Ankara/TR10/2011)

text: Influenza B virus (B/Ankara/TR10/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Sivas/TR14/2011)

text: Influenza B virus (B/Sivas/TR14/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Cameroon/LEID01111438/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Cameroon/11V12076GVFI/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Konya/TR01/2010)

text: Influenza B virus (B/Konya/TR01/2010)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Adana/TR06/2010)

text: Influenza B virus (B/Adana/TR06/2010)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Antalya/TR12/2011)

text: Influenza B virus (B/Antalya/TR12/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Ankara/TR21/2011)

text: Influenza B virus (B/Ankara/TR21/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Vilnius/704/2011)

text: Influenza B virus (B/Vilnius/704/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Skuodas/946/2011)

text: Influenza B virus (B/Skuodas/946/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Crimea/4980/2011)

text: Influenza B virus (B/Crimea/4980/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Donetsk/5188/2011)

text: Influenza B virus (B/Donetsk/5188/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Donetsk/5206/2011)

text: Influenza B virus (B/Donetsk/5206/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Iceland/48/2011)

text: Influenza B virus (B/Iceland/48/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/CameroonLEID/07111846/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Cameroon/13V-16387GVF1/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Cameroon/11V-12080GVF1/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Madagascar/3866/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Madagascar/8699/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/HongKong/748/2011)

text: Influenza B virus (B/HongKong/748/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/HongKong/767/2011)

text: Influenza B virus (B/HongKong/767/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Donetsk/4546/2011)

text: Influenza B virus (B/Donetsk/4546/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Odessa/4709/2011)

text: Influenza B virus (B/Odessa/4709/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Dnepropetrovsk/4719/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Dnepropetrovsk/4720/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/HongKong/568/2011)

text: Influenza B virus (B/HongKong/568/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/HongKong/599/2011)

text: Influenza B virus (B/HongKong/599/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/HongKong/762/2011)

text: Influenza B virus (B/HongKong/762/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Hmelnizk/4637/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Odessa/4705/2010)

text: Influenza B virus (B/Odessa/4705/2010)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/HongKong/765/2011)

text: Influenza B virus (B/HongKong/765/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Ghana/850/2011)

text: Influenza B virus (B/Ghana/850/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Slovenia/1141/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Dnepropetrovsk/4411/2010)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/LyonCHU/14767/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/LyonCHU/15731/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/LyonCHU/16167/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Ghana/516/2011)

text: Influenza B virus (B/Ghana/516/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Ghana/735/2011)

text: Influenza B virus (B/Ghana/735/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Slovenia/819/2011)

text: Influenza B virus (B/Slovenia/819/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Slovenia/1010/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Lyon/1179/2011)

text: Influenza B virus (B/Lyon/1179/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Ghana/494/2011)

text: Influenza B virus (B/Ghana/494/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Poitiers/267/2011)

text: Influenza B virus (B/Poitiers/267/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/LyonCHU/1288/2011)

text: Influenza B virus (B/LyonCHU/1288/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Vitebsk/672/2011)

text: Influenza B virus (B/Vitebsk/672/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Minsk/1559/2011)

text: Influenza B virus (B/Minsk/1559/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Ireland/132/2010)

text: Influenza B virus (B/Ireland/132/2010)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/HongKong/1889/2010)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Malta/MV457/2011)

text: Influenza B virus (B/Malta/MV457/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Norway/490/2011)

text: Influenza B virus (B/Norway/490/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Malmoe/1/2011)

text: Influenza B virus (B/Malmoe/1/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Norway/1188/2011)

text: Influenza B virus (B/Norway/1188/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Slovakia/193/2011)

text: Influenza B virus (B/Slovakia/193/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Slovakia/302/2011)

text: Influenza B virus (B/Slovakia/302/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Stockholm/14/2011)

text: Influenza B virus (B/Stockholm/14/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Malmoe/2/2011)

text: Influenza B virus (B/Malmoe/2/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Norway/1276/2011)

text: Influenza B virus (B/Norway/1276/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/HongKong/591/2011)

text: Influenza B virus (B/HongKong/591/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Serbia/2049)

text: Influenza B virus (B/Serbia/2049)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Serbia/2061)

text: Influenza B virus (B/Serbia/2061)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Serbia/2089)

text: Influenza B virus (B/Serbia/2089)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/England/169/2011)

text: Influenza B virus (B/England/169/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/England/172/2011)

text: Influenza B virus (B/England/172/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Turkey/682/2011)

text: Influenza B virus (B/Turkey/682/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Turkey/674/2011)

text: Influenza B virus (B/Turkey/674/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Serbia/2094)

text: Influenza B virus (B/Serbia/2094)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Serbia/2095)

text: Influenza B virus (B/Serbia/2095)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Madagascar/0236/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/England/684/2010)

text: Influenza B virus (B/England/684/2010)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/England/168/2011)

text: Influenza B virus (B/England/168/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Ireland/21653/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Ukraine/30/2011)

text: Influenza B virus (B/Ukraine/30/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Ukraine/142/2011)

text: Influenza B virus (B/Ukraine/142/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Ukraine/7/2011)

text: Influenza B virus (B/Ukraine/7/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Madagascar/1119/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Madagascar/0245/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Malta/MV000738/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Voronezh/20/2011)

text: Influenza B virus (B/Voronezh/20/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Kharkov/4178/2010)

text: Influenza B virus (B/Kharkov/4178/2010)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Kharkov/4260/2010)

text: Influenza B virus (B/Kharkov/4260/2010)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Hmelnizk/4273/2010)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Doneck/4353/2011)

text: Influenza B virus (B/Doneck/4353/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Moldova/284/2011)

text: Influenza B virus (B/Moldova/284/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Athens/10051/2011)

text: Influenza B virus (B/Athens/10051/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/CzechRep/36/2011)

text: Influenza B virus (B/CzechRep/36/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Ghana/FS11244/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Estonia/55784/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Murmansk/3/2011)

text: Influenza B virus (B/Murmansk/3/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/StPetersburg/5/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Algeria/G44/2010)

text: Influenza B virus (B/Algeria/G44/2010)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Turkey/103/2011)

text: Influenza B virus (B/Turkey/103/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Turkey/97/2011)

text: Influenza B virus (B/Turkey/97/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Turkey/93/2011)

text: Influenza B virus (B/Turkey/93/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Cameroon/10v-4954/2010)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/LaRioja/RR6969/2010)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Ceuta/RR7185/2010)

text: Influenza B virus (B/Ceuta/RR7185/2010)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Ceuta/RR7186/2011)

text: Influenza B virus (B/Ceuta/RR7186/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Milano/10/2011)

text: Influenza B virus (B/Milano/10/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Parma/04/2010)

text: Influenza B virus (B/Parma/04/2010)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Trieste/05/2011)

text: Influenza B virus (B/Trieste/05/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Lisboa/36/2010)

text: Influenza B virus (B/Lisboa/36/2010)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Lisboa/56/2010)

text: Influenza B virus (B/Lisboa/56/2010)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Lisboa/4/2011)

text: Influenza B virus (B/Lisboa/4/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Israel/4-11/2011)

text: Influenza B virus (B/Israel/4-11/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Valladolid/57/2010)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Slovenia/46/2011)

text: Influenza B virus (B/Slovenia/46/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Lyon/74/2011)

text: Influenza B virus (B/Lyon/74/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Lyon/68/2011)

text: Influenza B virus (B/Lyon/68/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Geneva/4201882/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Parma/3/2011)

text: Influenza B virus (B/Parma/3/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/England/170/2010)

text: Influenza B virus (B/England/170/2010)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/England/512/2010)

text: Influenza B virus (B/England/512/2010)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/England/421/2010)

text: Influenza B virus (B/England/421/2010)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Valladolid10/2011)

text: Influenza B virus (B/Valladolid10/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/CotedIvoire/GR1576/2010)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/CotedIvoire/GR1655/2010)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Washington/01/2010)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Georgia/75/2011)

text: Influenza B virus (B/Georgia/75/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Genoa/02/2010)

text: Influenza B virus (B/Genoa/02/2010)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Ireland/0132/2010)

text: Influenza B virus (B/Ireland/0132/2010)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/CotedIvoire/GR1520/2010)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Finland/33/2010)

text: Influenza B virus (B/Finland/33/2010)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Finland/39/2010)

text: Influenza B virus (B/Finland/39/2010)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Brussels/G0710/2010)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Bretagne/2278/2010)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Paris/1850/2010)

text: Influenza B virus (B/Paris/1850/2010)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Agadir/156/2011)

text: Influenza B virus (B/Agadir/156/2011)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Latvia/12-43110/2010)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Brussels/G0707/2010)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Lisboa/14/2010)

text: Influenza B virus (B/Lisboa/14/2010)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Lisboa/20/2010)

text: Influenza B virus (B/Lisboa/20/2010)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/HongKong/2188/2010)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Niedersachsen/2/2010)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/NordrheinWestfalen/1/2010)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Berlin/1/2010)

text: Influenza B virus (B/Berlin/1/2010)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Berlin/2/2010)

text: Influenza B virus (B/Berlin/2/2010)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Lisboa/15/2010)

text: Influenza B virus (B/Lisboa/15/2010)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Lisboa/21/2010)

text: Influenza B virus (B/Lisboa/21/2010)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/HongKong/2200/2010)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/HongKong/2207/2010)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Latvia/12-41046/2010)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Egypt/46/2010)

text: Influenza B virus (B/Egypt/46/2010)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Egypt/66/2010)

text: Influenza B virus (B/Egypt/66/2010)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Egypt/65/2010)

text: Influenza B virus (B/Egypt/65/2010)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Zamora/52/2010)

text: Influenza B virus (B/Zamora/52/2010)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Tehran/5246/2010)

text: Influenza B virus (B/Tehran/5246/2010)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Tehran/5277/2010)

text: Influenza B virus (B/Tehran/5277/2010)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Madagascar/7766/2010)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Luxembourg/558/2010)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Lisboa/8/2010)

text: Influenza B virus (B/Lisboa/8/2010)


text: Influenzavirus B

text: SC

text: public

text: Influenzavirus B

text: Influenza B virus (B/Lisboa/13/2010)

text: Influenza B virus (B/Lisboa/13/2010)


text: public

tag: Namespace:Centre for Integrative Physiology, University of Edinburgh

text: ArrayExpress

characteristic: broker name

text: neuron

characteristic: cell type

text: house mouse

text: Protocols: Astrocytes and neurons were cultured from E17.5 CD1 mouse and E20.5 Sprague Dawley rat embryos. Cortices were dissected, enzymatically digested with papain and mechanically dissociated using a 2 mL pipette. Mouse astrocytes were obtained by growing cells at low density in DMEM containing 10% serum and were passaged twice, using Trypsin (both Life Technologies), before co-culturing them with neurons in a 24-well plate. These astrocytes are >99% GFAP positive and <0.1% NeuN positive. For mixed-species co-cultures, rat neurons were plated on top of a confluent layer of DIV14 mouse astrocytes and both astrocyte mono-cultures and astrocyte-neuron co-cultures were subsequently kept in Neurobasal-A medium containing B27 (both Life Technologies), but devoid of serum. Where used, pure neuronal cultures were generated from E17.5 CD1 mouse embryos, with the anti-mitotic AraC added on the day of plating which restricts astrocyte numbers to <0.1%. All cultures were used 8-10 days post neuron plate down. Cells were transferred into media containing 10% Minimum Essential Media (MEM, Life Technologies) and 90% Salt-Glucose-Glycine (SGG) medium containing 114 mM NaCl, 0.219 % NaHCO3, 5.292 mM KCl, 1 mM MgCl2, 2 mM CaCl2, 10 mM HEPES, 1 mM Glycine, 30 mM Glucose, 0.5 mM sodium pyruvate, 0.1 % Phenol Red; osmolarity 325 mosm/l) either over night or for 4h prior to stimulations. To investigate the effect of synaptic activity on astrocytic gene expression, co-cultures were transferred into TTX-containing medium on DIV8 for 22h to inhibit neuronal action potential firing. Subsequently, cells were washed with medium free of TTX and then kept in medium either containing Bicuculline (BiC, 50 μM, Tocris) to induce network bursting, or BiC and TBOA (DL-threo-β-Benzyloxyaspartic acid, 50 μM, Tocris), to increase the half-time of glutamate uptake, for 16h, after which RNA was collected. Control conditions were washed with TTX and left in medium containing either BiC or BiC/TBOA in the presence of TTX, or TTX alone. Barcoded RNA-seq libraries were prepared by Edinburgh Genomics using the Illumina TruSeq stranded mRNA-seq kit, according to the manufacturer’s protocol (Illumina).

characteristic: description

text: monoculture

characteristic: growth condition

text: Mus musculus

characteristic: neuron species

text: Mus musculus

text: Mus musculus

text: Mus musculus

type: has member


text: Centre for Integrative Physiology, University of Edinburgh

text: public

text: ArrayExpress

text: neuron

text: house mouse

text: monoculture

text: Mus musculus

text: Mus musculus

characteristic: organism

text: Mus musculus

text: Mus musculus

characteristic: title


characteristic: ENA-CHECKLIST

text: public

text: Mus musculus

characteristic: astrocyte species

text: ArrayExpress

text: mixed astrocyte and neuron

text: house mouse

characteristic: common name

text: monoculture

text: Mus musculus (simulated 5% contamination)

text: Mus musculus

text: Mus musculus

text: Mus musculus


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: Mus musculus

text: ArrayExpress

text: astrocyte

text: house mouse

text: monoculture

text: Mus musculus

text: Mus musculus

text: Mus musculus


characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: Mus musculus

text: ArrayExpress

text: mixed astrocyte and neuron

text: co-culture

text: Rattus norvegicus

text: mixed sample

text: mixed sample

text: mixed sample


text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Rattus norvegicus

text: ArrayExpress

text: mixed astrocyte and neuron

text: Norway rat

text: co-culture

text: Rattus norvegicus

text: Rattus norvegicus

text: Rattus norvegicus

text: Rattus norvegicus

text: Ratonly


text: TAGC - INSERM U1090

text: public

tag: Namespace:TAGC - INSERM U1090

unit: day

characteristic: age

text: ArrayExpress

characteristic: broker name

text: fruit fly

text: Protocols: Fly rearing was performed on standard cornmeal agar yeast food and the flies were raised at 25°C Library Selection: Poly(A)+, Library Strand: First Strand

characteristic: description

text: w1118; canton S isogenic line

characteristic: genotype

text: Drosophila melanogaster

text: Drosophila melanogaster

text: whole organism

characteristic: organism part

text: inflammation

characteristic: phenotype

text: Drosophila melanogaster

text: female

characteristic: sex


characteristic: ENA-CHECKLIST

text: TAGC - INSERM U1090

text: public

text: ArrayExpress

text: fruit fly

characteristic: common name

text: w1118; canton S isogenic line

text: Drosophila melanogaster

characteristic: organism

text: Drosophila melanogaster

text: whole organism

text: inflammation and infection

text: Drosophila melanogaster

text: female

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: TAGC - INSERM U1090

characteristic: INSDC center name

text: public

text: ArrayExpress

text: fruit fly

text: w1118; canton S isogenic line

text: Drosophila melanogaster

text: Drosophila melanogaster

text: whole organism

text: infection

text: Drosophila melanogaster

text: female


characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:MAX PLANCK INSTITUTE OF IMMUNOBIOLOGY AND EPIGENETICS

text: fruit fly

text: sequenced in two different sequencing runs

text: embryos before cycle 9

characteristic: dev stage

text: Drosophila melanogaster

text: Drosophila melanogaster


text: MAX PLANCK INSTITUTE OF IMMUNOBIOLOGY AND EPIGENETICS

text: public

characteristic: Submitter Id

text: fruit fly

characteristic: description

text: embryos before cycle 9

text: Drosophila melanogaster

text: Drosophila melanogaster


text: UNIVERSITY OF ABERDEEN

text: public

text: Campylobacter coli

text: Campylobacter coli

text: iCaMPS-4 study chicken isolate


text: Neogen

text: public

text: human

text: Homo sapiens

text: Homo sapiens

text: Dummy-sample


text: UMCG

text: public

characteristic: collection date

text: Germany

characteristic: geographic location (country and/or sea)

text: diseased

characteristic: host health state

text: homo sapiens

characteristic: host scientific name

characteristic: isolate

text: Clinical isolate

characteristic: isolation source

text: Latitude: 47°59.754′ N Longitude: 7°51.1332′ E

characteristic: lat lon

text: Escherichia coli

text: Escherichia coli

text: AcrAB-TolC inactivation in an MDR E.coli clinical isolate


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: ARS-USDA

text: public

text: Department of Animal Health, Ministry of Agriculture and Rural Development, Ho Chi Minh City, Vietnam.

characteristic: collecting institution

text: Regional Animal Health Office No. 6

characteristic: collector name

text: The sample was isolated from an epithelial lesion of a pig collected in September 2015 in Đắk Lắk province, Vietnam associated with acute and rapid onset of vesicular disease amongst 45 of the 50 pigs present in the affected farm.

text: Viet Nam

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: Dak Lak

characteristic: geographic location (region and locality)

unit: year

characteristic: host age

text: domestic

characteristic: host behaviour

text: pig

characteristic: host common name

text: diseased

text: Sus scrofa domesticus

text: not collected

characteristic: host sex

characteristic: host subject id

text: Foot-and-mouth disease virus - type O

characteristic: organism

characteristic: receipt date

text: active surveillance in response to outbreak

characteristic: sample capture status

text: Foot-and-mouth disease virus - type O

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: External Id

text: ITQB

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: Netherlands

characteristic: geographic location (country and/or sea)

text: not provided

characteristic: host health state

text: Equus caballus

characteristic: host scientific name

text: Horse

characteristic: isolation source

text: Staphylococcus fleurettii

text: Staphylococcus fleurettii

text: Staphylococcus sciuri group WGS


Zymaflore F15

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: UCIBIO

text: public

text: Zymaflore F15

text: baker's yeast

characteristic: common name

text: Commercial yeast (red wine)

characteristic: description

text: Saccharomyces cerevisiae

characteristic: organism

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae wine yeast


IOC 18-2007

characteristic: ENA-CHECKLIST

characteristic: External Id

text: UCIBIO

text: public

text: IOC 18-2007

text: baker's yeast

text: Commercial yeast

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae wine yeast

characteristic: title


Lalvin BM45

text: UCIBIO

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: Lalvin BM45

text: baker's yeast

text: Commercial yeast (white wine)

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae wine yeast


Lalvin CY-3079

text: UCIBIO

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Lalvin CY-3079

characteristic: Submitter Id

text: baker's yeast

text: Commercial yeast (red wine)

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae wine yeast


Lalvin W15

text: UCIBIO

text: public

text: Lalvin W15

text: baker's yeast

text: Commercial yeast (white and red wine)

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae wine yeast


PR

text: UCIBIO

text: public

text: PR

text: baker's yeast

text: Pasteur Red

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae wine yeast


PYCC 4072

text: UCIBIO

text: public

text: PYCC 4072

text: baker's yeast

text: commercial wine yeast (Fermivin)

text: Portugal

characteristic: geographic location (country and/or sea)

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae wine yeast


PYCC 4074

text: UCIBIO

text: public

text: PYCC 4074

text: baker's yeast

text: commercial wine yeast (Fermichamp)

text: Portugal

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae wine yeast


TUM V1

text: UCIBIO

text: public

text: TUM V1

text: baker's yeast

text: Bordeaux wine

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae wine yeast


Uvaferm SGV

text: UCIBIO

text: public

text: Uvaferm SGV

text: baker's yeast

text: Commercial yeast ( red wine)

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae wine yeast


Uvaferm VRB

text: UCIBIO

text: public

text: Uvaferm VRB

text: baker's yeast

text: Commercial yeast ( red wine)

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae wine yeast


PYCC 6722

text: UCIBIO

text: public

text: PYCC 6722

text: baker's yeast

text: wine

text: Armenia

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae wine yeast


PYCC 6726

text: UCIBIO

text: public

text: PYCC 6726

text: baker's yeast

text: Jerez-wine

text: Spain

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae wine yeast


PYCC 6729

text: UCIBIO

text: public

text: PYCC 6729

text: baker's yeast

text: Jerez-wine

text: Armenia

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae

text: Saccharomyces cerevisiae wine yeast


text: public

tag: Namespace:University of Chinese Academy of Sciences

characteristic: collection date

text: wheat and maize

characteristic: environment (biome)

text: agricultural field

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: China

characteristic: geographic location (depth)

characteristic: geographic location (elevation)

text: 37.53 N

characteristic: geographic location (latitude)

text: 114.41 E

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: soil metagenome

text: 16S rRNA

characteristic: project name

text: soil metagenome

text: Illumina

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: first layer for original soil


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Chinese Academy of Sciences

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: wheat and maize

text: agricultural field

text: soil

text: China

text: 37.53 N

text: 114.41 E

text: metagenome

text: soil metagenome

characteristic: organism

text: 16S rRNA

text: soil metagenome

text: Illumina

text: soil

text: first layer for original soil

characteristic: title


text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

text: wheat and maize

characteristic: environment (biome)

text: agricultural field

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: China

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

text: 37.53 N

text: 114.41 E

text: metagenome

text: soil metagenome

text: 16S rRNA

text: soil metagenome

text: Illumina

text: soil

text: first layer for aerobic incubation


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: wheat and maize

text: agricultural field

text: soil

text: China

characteristic: geographic location (elevation)

text: 37.53 N

characteristic: geographic location (latitude)

text: 114.41 E

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: soil metagenome

text: 16S rRNA

characteristic: project name

text: soil metagenome

text: Illumina

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: first layer for anaerobic incubation


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: wheat and maize

text: agricultural field

text: soil

text: China

text: 37.53 N

text: 114.41 E

text: metagenome

text: soil metagenome

characteristic: organism

text: 16S rRNA

text: soil metagenome

text: Illumina

text: soil

text: first layer for aerobic incubation with glutamate sodium

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: Submitter Id

characteristic: collection date

text: wheat and maize

characteristic: environment (biome)

text: agricultural field

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: China

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

text: 37.53 N

text: 114.41 E

text: metagenome

text: soil metagenome

text: 16S rRNA

text: soil metagenome

text: Illumina

text: soil

text: first layer for anaerobic incubation with glutamate sodium


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: wheat and maize

text: agricultural field

text: soil

text: China

characteristic: geographic location (elevation)

text: 37.53 N

characteristic: geographic location (latitude)

text: 114.41 E

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: soil metagenome

text: 16S rRNA

characteristic: project name

text: soil metagenome

text: Illumina

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: second layer for original soil


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: wheat and maize

text: agricultural field

text: soil

text: China

text: 37.53 N

text: 114.41 E

text: metagenome

text: soil metagenome

characteristic: organism

text: 16S rRNA

text: soil metagenome

text: Illumina

text: soil

text: second layer for aerobic incubation

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: Submitter Id

characteristic: collection date

text: wheat and maize

characteristic: environment (biome)

text: agricultural field

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: China

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

text: 37.53 N

text: 114.41 E

text: metagenome

text: soil metagenome

text: 16S rRNA

text: soil metagenome

text: Illumina

text: soil

text: second layer for anaerobic incubation


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: wheat and maize

text: agricultural field

text: soil

text: China

characteristic: geographic location (elevation)

text: 37.53 N

characteristic: geographic location (latitude)

text: 114.41 E

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: soil metagenome

text: 16S rRNA

characteristic: project name

text: soil metagenome

text: Illumina

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: second layer for aerobic incubation with glutamate sodium


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: wheat and maize

text: agricultural field

text: soil

text: China

text: 37.53 N

text: 114.41 E

text: metagenome

text: soil metagenome

characteristic: organism

text: 16S rRNA

text: soil metagenome

text: Illumina

text: soil

text: second layer for anaerobic incubation with glutamate sodium

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: Submitter Id

characteristic: collection date

text: wheat and maize

characteristic: environment (biome)

text: agricultural field

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: China

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

text: 37.53 N

text: 114.41 E

text: metagenome

text: soil metagenome

text: 16S rRNA

text: soil metagenome

text: Illumina

text: soil

text: third layer for original soil


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: wheat and maize

text: agricultural field

text: soil

text: China

characteristic: geographic location (elevation)

text: 37.53 N

characteristic: geographic location (latitude)

text: 114.41 E

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: soil metagenome

text: 16S rRNA

characteristic: project name

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characteristic: sequencing method

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characteristic: soil environmental package

text: third layer for aerobic incubation soil


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: wheat and maize

text: agricultural field

text: soil

text: China

text: 37.53 N

text: 114.41 E

text: metagenome

text: soil metagenome

characteristic: organism

text: 16S rRNA

text: soil metagenome

text: Illumina

text: soil

text: third layer for anaerobic incubation soil

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: Submitter Id

characteristic: collection date

text: wheat and maize

characteristic: environment (biome)

text: agricultural field

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: China

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

text: 37.53 N

text: 114.41 E

text: metagenome

text: soil metagenome

text: 16S rRNA

text: soil metagenome

text: Illumina

text: soil

text: third layer for aerobic incubation with glutamate sodium


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: wheat and maize

text: agricultural field

text: soil

text: China

characteristic: geographic location (elevation)

text: 37.53 N

characteristic: geographic location (latitude)

text: 114.41 E

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: soil metagenome

text: 16S rRNA

characteristic: project name

text: soil metagenome

text: Illumina

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: third layer for anaerobic incubation with glutamate sodium


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: wheat and maize

text: agricultural field

text: soil

text: China

text: 37.53 N

text: 114.41 E

text: metagenome

text: soil metagenome

characteristic: organism

text: 16S rRNA

text: soil metagenome

text: Illumina

text: soil

text: forth layer for original soil

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: Submitter Id

characteristic: collection date

text: wheat and maize

characteristic: environment (biome)

text: agricultural field

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: China

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

text: 37.53 N

text: 114.41 E

text: metagenome

text: soil metagenome

text: 16S rRNA

text: soil metagenome

text: Illumina

text: soil

text: forth layer for aerobic incubation soil


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: wheat and maize

text: agricultural field

text: soil

text: China

characteristic: geographic location (elevation)

text: 37.53 N

characteristic: geographic location (latitude)

text: 114.41 E

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: soil metagenome

text: 16S rRNA

characteristic: project name

text: soil metagenome

text: Illumina

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: forth layer for anaerobic incubation soil


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: wheat and maize

text: agricultural field

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text: China

text: 37.53 N

text: 114.41 E

text: metagenome

text: soil metagenome

characteristic: organism

text: 16S rRNA

text: soil metagenome

text: Illumina

text: soil

text: forth layer for aerobic incubation with glutamate sodium

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: Submitter Id

characteristic: collection date

text: wheat and maize

characteristic: environment (biome)

text: agricultural field

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: China

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

text: 37.53 N

text: 114.41 E

text: metagenome

text: soil metagenome

text: 16S rRNA

text: soil metagenome

text: Illumina

text: soil

text: forth layer for anaerobic incubation with glutamate sodium


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: wheat and maize

text: agricultural field

text: soil

text: China

characteristic: geographic location (elevation)

text: 37.53 N

characteristic: geographic location (latitude)

text: 114.41 E

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: soil metagenome

text: 16S rRNA

characteristic: project name

text: soil metagenome

text: Illumina

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: fifth layer for original soil


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: wheat and maize

text: agricultural field

text: soil

text: China

text: 37.53 N

text: 114.41 E

text: metagenome

text: soil metagenome

characteristic: organism

text: 16S rRNA

text: soil metagenome

text: Illumina

text: soil

text: fifth layer for aerobic incubation soil

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: Submitter Id

characteristic: collection date

text: wheat and maize

characteristic: environment (biome)

text: agricultural field

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: China

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

text: 37.53 N

text: 114.41 E

text: metagenome

text: soil metagenome

text: 16S rRNA

text: soil metagenome

text: Illumina

text: soil

text: fifth layer for anaerobic incubation soil


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: wheat and maize

text: agricultural field

text: soil

text: China

characteristic: geographic location (elevation)

text: 37.53 N

characteristic: geographic location (latitude)

text: 114.41 E

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: soil metagenome

text: 16S rRNA

characteristic: project name

text: soil metagenome

text: Illumina

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: fifth layer for aerobic incubation with glutamate sodium


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: wheat and maize

text: agricultural field

text: soil

text: China

text: 37.53 N

text: 114.41 E

text: metagenome

text: soil metagenome

characteristic: organism

text: 16S rRNA

text: soil metagenome

text: Illumina

text: soil

text: fifth layer for anaerobic incubation with glutamate sodium

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

characteristic: Submitter Id

characteristic: collection date

text: wheat and maize

characteristic: environment (biome)

text: agricultural field

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: China

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

text: 37.53 N

text: 114.41 E

text: metagenome

text: soil metagenome

text: 16S rRNA

text: soil metagenome

text: Illumina

text: soil

text: fifth layer for ANaerobic incubation with glutamate sodium


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

text: wheat and maize

text: agricultural field

text: soil

text: China

characteristic: geographic location (elevation)

text: 37.53 N

characteristic: geographic location (latitude)

text: 114.41 E

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: soil metagenome

text: 16S rRNA

characteristic: project name

text: soil metagenome

text: Illumina

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: sixth layer for original soil


characteristic: ENA-LAST-UPDATE

characteristic: External Id

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: wheat and maize

text: agricultural field

text: soil

text: China

text: 37.53 N

text: 114.41 E

text: metagenome

text: soil metagenome

characteristic: organism

text: 16S rRNA

text: soil metagenome

text: Illumina

text: soil

text: sixth layer for aerobic incubation soil

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: public

characteristic: Submitter Id

characteristic: collection date

text: wheat and maize

characteristic: environment (biome)

text: agricultural field

characteristic: environment (feature)

text: soil

characteristic: environment (material)

text: China

characteristic: geographic location (country and/or sea)

characteristic: geographic location (depth)

text: 37.53 N

text: 114.41 E

text: metagenome

text: soil metagenome

text: 16S rRNA

text: soil metagenome

text: Illumina

text: soil

text: sixth layer for anaerobic incubation soil


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: wheat and maize

text: agricultural field

text: soil

text: China

characteristic: geographic location (elevation)

text: 37.53 N

characteristic: geographic location (latitude)

text: 114.41 E

characteristic: geographic location (longitude)

text: metagenome

characteristic: investigation type

text: soil metagenome

text: 16S rRNA

characteristic: project name

text: soil metagenome

text: Illumina

characteristic: sequencing method

text: soil

characteristic: soil environmental package

text: sixth layer for aerobic incubation with glutamate sodium


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: University of Chinese Academy of Sciences

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: wheat and maize

text: agricultural field

text: soil

text: China

text: 37.53 N

text: 114.41 E

text: metagenome

text: soil metagenome

characteristic: organism

text: 16S rRNA

text: soil metagenome

text: Illumina

text: soil

text: sixth layer for anaerobic incubation with glutamate sodium

characteristic: title


E-MTAB-5526:delta-nc35 biofilm 1

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

text: E-MTAB-5526:delta-nc35 biofilm 1

tag: Namespace:Laboratory of Pharmaceutical Microbiology  Department of Pharmaceutical Sciences Ghent University

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: Protocols: Planktonic cultures were harvested by centrifugation, at cell density of 5 x 10^8 for exponential phase and 2 x 10^9 for stationary phase. Biofilms were washed, saline solution added to wells and cells detached by sonication and shaking. Cells from the saline solution were collected by centrifugation. Planktonic cultures were grown in LB, in 25 ml batches in a 250 ml flask, on a shaking incubator at 150 rpm and 37 degrees C. Biofilm cultures were grown in microtiter plates in LB. After 4 hours incubation biofilms were washed and then further incubated in fresh medium for a combined 24h. Plates were incubated statically at 37 degrees C. RNA was extracted using the RiboPure bacteria kit (Invitrogen), with the following modification to standard protocol: the proportion of ethanol added to crude extract prior to clean-up step was increased to 1.25 x to retain more small RNAs. Before DNase treatment, RNA was denatured by heating to 65°C for 5 min and DNase incubation time was increased from 30 min to 60 min. Libraries were prepared from ribo-depleted total RNA using the 'Truseq stranded Total RNA library prep' (Illumina) according to manufacturer's protocol.

characteristic: description

text: deletion ncS35

characteristic: genotype

text: biofilm

characteristic: growth condition

text: Burkholderia cenocepacia

text: Burkholderia cenocepacia

text: Burkholderia cenocepacia

text: delta-nc35 biofilm 1


E-MTAB-5526:delta-nc35 biofilm 2

text: Laboratory of Pharmaceutical Microbiology Department of Pharmaceutical Sciences Ghent University

text: public

text: E-MTAB-5526:delta-nc35 biofilm 2

text: ArrayExpress

text: deletion ncS35

text: biofilm

text: Burkholderia cenocepacia

text: Burkholderia cenocepacia

text: Burkholderia cenocepacia

text: delta-nc35 biofilm 2


E-MTAB-5526:delta-nc35 biofilm 3

text: public

text: E-MTAB-5526:delta-nc35 biofilm 3

text: ArrayExpress

text: deletion ncS35

text: biofilm

text: Burkholderia cenocepacia

text: Burkholderia cenocepacia

text: Burkholderia cenocepacia

text: delta-nc35 biofilm 3


E-MTAB-5526:delta-nc35 exponential 1

text: public

text: E-MTAB-5526:delta-nc35 exponential 1

text: ArrayExpress

text: deletion ncS35

text: exponential phase

text: Burkholderia cenocepacia

text: Burkholderia cenocepacia

text: Burkholderia cenocepacia

text: delta-nc35 exponential 1


E-MTAB-5526:delta-nc35 exponential 2

text: public

text: E-MTAB-5526:delta-nc35 exponential 2

text: ArrayExpress

text: deletion ncS35

text: exponential phase

text: Burkholderia cenocepacia

text: Burkholderia cenocepacia

text: Burkholderia cenocepacia

text: delta-nc35 exponential 2


E-MTAB-5526:delta-nc35 exponential 3

text: public

text: E-MTAB-5526:delta-nc35 exponential 3

text: ArrayExpress

text: deletion ncS35

text: exponential phase

text: Burkholderia cenocepacia

text: Burkholderia cenocepacia

text: Burkholderia cenocepacia

text: delta-nc35 exponential 3


E-MTAB-5526:delta-nc35 stationary 1

text: public

text: E-MTAB-5526:delta-nc35 stationary 1

text: ArrayExpress

text: deletion ncS35

text: stationary phase

text: Burkholderia cenocepacia

text: Burkholderia cenocepacia

text: Burkholderia cenocepacia

text: delta-nc35 stationary 1


E-MTAB-5526:delta-nc35 stationary 2

text: public

text: E-MTAB-5526:delta-nc35 stationary 2

text: ArrayExpress

text: deletion ncS35

text: stationary phase

text: Burkholderia cenocepacia

text: Burkholderia cenocepacia

text: Burkholderia cenocepacia

text: delta-nc35 stationary 2


E-MTAB-5526:delta-nc35 stationary 3

text: public

text: E-MTAB-5526:delta-nc35 stationary 3

text: ArrayExpress

text: deletion ncS35

text: stationary phase

text: Burkholderia cenocepacia

text: Burkholderia cenocepacia

text: Burkholderia cenocepacia

text: delta-nc35 stationary 3


E-MTAB-5526:wild type biofilm 1

text: public

text: E-MTAB-5526:wild type biofilm 1

text: ArrayExpress

text: wild type genotype

text: biofilm

text: Burkholderia cenocepacia

text: Burkholderia cenocepacia

text: Burkholderia cenocepacia

text: wild type biofilm 1


E-MTAB-5526:wild type biofilm 2

text: public

text: E-MTAB-5526:wild type biofilm 2

text: ArrayExpress

text: wild type genotype

text: biofilm

text: Burkholderia cenocepacia

text: Burkholderia cenocepacia

text: Burkholderia cenocepacia

text: wild type biofilm 2


E-MTAB-5526:wild type biofilm 3

text: public

text: E-MTAB-5526:wild type biofilm 3

text: ArrayExpress

text: wild type genotype

text: biofilm

text: Burkholderia cenocepacia

text: Burkholderia cenocepacia

text: Burkholderia cenocepacia

text: wild type biofilm 3


E-MTAB-5526:wild type exponential 1

text: public

text: E-MTAB-5526:wild type exponential 1

text: ArrayExpress

text: wild type genotype

text: exponential phase

text: Burkholderia cenocepacia

text: Burkholderia cenocepacia

text: Burkholderia cenocepacia

text: wild type exponential 1


E-MTAB-5526:wild type exponential 2

text: public

text: E-MTAB-5526:wild type exponential 2

text: ArrayExpress

text: wild type genotype

text: exponential phase

text: Burkholderia cenocepacia

text: Burkholderia cenocepacia

text: Burkholderia cenocepacia

text: wild type exponential 2


E-MTAB-5526:wild type exponential 3

text: public

text: E-MTAB-5526:wild type exponential 3

text: ArrayExpress

text: wild type genotype

text: exponential phase

text: Burkholderia cenocepacia

text: Burkholderia cenocepacia

text: Burkholderia cenocepacia

text: wild type exponential 3


E-MTAB-5526:wild type stationary 1

text: public

text: E-MTAB-5526:wild type stationary 1

text: ArrayExpress

text: wild type genotype

text: stationary phase

text: Burkholderia cenocepacia

text: Burkholderia cenocepacia

text: Burkholderia cenocepacia

text: wild type stationary 1


E-MTAB-5526:wild type stationary 2

text: public

text: E-MTAB-5526:wild type stationary 2

text: ArrayExpress

text: wild type genotype

text: stationary phase

text: Burkholderia cenocepacia

text: Burkholderia cenocepacia

text: Burkholderia cenocepacia

text: wild type stationary 2


E-MTAB-5526:wild type stationary 3

text: public

text: E-MTAB-5526:wild type stationary 3

text: ArrayExpress

text: wild type genotype

text: stationary phase

text: Burkholderia cenocepacia

text: Burkholderia cenocepacia

text: Burkholderia cenocepacia

text: wild type stationary 3


text: Forensic and Scientific Services

text: public

tag: Namespace:Forensic and Scientific Services

text: Clostridium tetani

text: Clostridium tetani

text: C. tetani


text: Institute for Molecular Biosciences

text: public

tag: Namespace:Institute for Molecular Biosciences

text: BRAEMBL

characteristic: collection date

text: Nucleic acid sequences and peptide sequences from venom-gland preparations from the spider Liphistius sp

text: Germany

characteristic: geographic location (country and/or sea)

characteristic: lat lon

text: Liphistius sp. SGP-2016

text: Aerobe

characteristic: relationship to oxygen

text: Spiders were purchased from a collector in Germany

characteristic: sample collection device or method

text: Liphistius sp. SGP-2016

text: LiphistiusS venom gland


characteristic: ENA-CHECKLIST

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