text: University of Bath

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: No

text: United Kingdom

text: 51.30 N

text: 0.7 W

characteristic: geographic location (longitude)

text: diseased

text: Phasianus colchicus

text: Yes

text: Staphylococcus aureus

characteristic: organism

text: Staphylococcus aureus


status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Bath

characteristic: INSDC center name

text: public

tag: Namespace:University of Bath

text: J Ross Fitzgerald at The Roslin Institute and Centre for Infectious Diseases, University of Edinburgh

characteristic: collected by

characteristic: collection date

text: No

characteristic: environmental sample

characteristic: genotype

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: 51.3 N

unit: DD

characteristic: geographic location (latitude)

text: 0.7 W

text: diseased

characteristic: host health state

text: Phasianus colchicus

characteristic: host scientific name

text: Yes

characteristic: is the sequenced pathogen host associated?

characteristic: isolate

text: Staphylococcus aureus

text: Staphylococcus aureus

text: Poultry-associated Staphylococcus aureus

characteristic: title


text: University of Bath

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: No

text: United Kingdom

text: 51.3 N

text: 07 W

characteristic: geographic location (longitude)

text: diseased

text: Phasianus colchicus

text: Yes

text: Staphylococcus aureus

characteristic: organism

text: Staphylococcus aureus


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Bath

characteristic: INSDC center name

text: public

characteristic: collected by

characteristic: collection date

text: No

text: United Kingdom

text: 51.3 N

text: 0.7 W

text: not provided

text: Gallus gallus

text: Yes

text: Staphylococcus aureus

text: Staphylococcus aureus


text: University of Bath

text: public

text: No

characteristic: environmental sample

characteristic: genotype

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: 51.3 N

characteristic: geographic location (latitude)

text: 0.7 W

text: diseased

characteristic: host health state

text: Gallus gallus

characteristic: host scientific name

text: Yes

characteristic: is the sequenced pathogen host associated?

text: Staphylococcus aureus

text: Staphylococcus aureus


text: University of Bath

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: No

text: United Kingdom

text: 51.3 N

text: 0.7 W

text: diseased

text: Perdix perdix

text: Yes

characteristic: isolate

text: Staphylococcus aureus

text: Staphylococcus aureus

characteristic: title


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Bath

characteristic: INSDC center name

text: public

text: No

text: United Kingdom

text: 51.3 N

text: 0.7 W

characteristic: geographic location (longitude)

text: diseased

text: Meleagris gallopavo

text: Yes

text: Staphylococcus aureus

characteristic: organism

text: Staphylococcus aureus


text: University of Bath

text: public

characteristic: collected by

characteristic: collection date

text: No

characteristic: environmental sample

characteristic: genotype

text: Belgium

characteristic: geographic location (country and/or sea)

text: 50.51 N

characteristic: geographic location (latitude)

text: 4.21 E

text: healthy

characteristic: host health state

text: Gallus gallus

text: Yes

text: AV CIX 38/61274

text: Staphylococcus aureus

text: Staphylococcus aureus


text: University of Bath

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: No

text: Belgium

text: 50.51 N

text: 4.21 E

text: healthy

text: Gallus gallus

characteristic: host scientific name

text: Yes

characteristic: is the sequenced pathogen host associated?

text: AV CIX 18/61273

text: Staphylococcus aureus

text: Staphylococcus aureus


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Bath

characteristic: INSDC center name

text: public

text: No

text: United Kingdom

text: 51.3 N

text: 0.7 W

text: diseased

text: Gallus gallus

text: Yes

text: AV 86/61269

characteristic: isolate

text: Staphylococcus aureus

text: Staphylococcus aureus

characteristic: title


text: University of Bath

text: public

characteristic: collected by

characteristic: collection date

text: No

characteristic: environmental sample

characteristic: genotype

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: 51.3 N

text: 0.7 W

characteristic: geographic location (longitude)

text: diseased

text: Gallus gallus

text: Yes

text: AV 4/61267

text: Staphylococcus aureus

characteristic: organism

text: Staphylococcus aureus


text: University of Bath

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: No

text: United Kingdom

text: 51.3 N

characteristic: geographic location (latitude)

text: 0.7 W

text: diseased

characteristic: host health state

text: Gallus gallus

text: Yes

text: AV 24/61268

text: Staphylococcus aureus

text: Staphylococcus aureus


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Bath

characteristic: INSDC center name

text: public

text: No

text: USA

text: 38.54 N

text: 77.0 W

text: diseased

text: Gallus gallus

characteristic: host scientific name

text: Yes

characteristic: is the sequenced pathogen host associated?

text: AV 10009/61272

text: Staphylococcus aureus

text: Staphylococcus aureus


text: University of Bath

text: public

characteristic: collected by

characteristic: collection date

text: No

characteristic: environmental sample

characteristic: genotype

text: USA

characteristic: geographic location (country and/or sea)

text: 38.54 N

text: 77.0 W

text: diseased

text: Gallus gallus

text: Yes

text: AV 00-8661/61270

characteristic: isolate

text: Staphylococcus aureus

text: Staphylococcus aureus

characteristic: title


text: University of Bath

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: No

text: USA

text: 38.54 N

text: 77.0 W

characteristic: geographic location (longitude)

text: diseased

text: Gallus gallus

text: Yes

text: AV 00-7439/61271

text: Staphylococcus aureus

characteristic: organism

text: Staphylococcus aureus


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Bath

characteristic: INSDC center name

text: public

text: No

text: Australia

text: 33.51 S

characteristic: geographic location (latitude)

text: 151.12 E

text: healthy

characteristic: host health state

text: Gallus gallus

text: Yes

text: Staphylococcus aureus

text: Staphylococcus aureus


text: public

tag: Namespace:LEIBNIZ CENTRE FOR TROPICAL MARINE ECOLOGY

text: Bacterial community sample from the inhabited island Barrang Lompo, north transect, water column, free-living fraction, 25m distance from island

characteristic: description

text: Yes

characteristic: environmental sample

text: Indonesia

characteristic: geographic location (country and/or sea)

text: Spermonde Archipelago

characteristic: geographic location (region and locality)

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: LEIBNIZ CENTRE FOR TROPICAL MARINE ECOLOGY

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: Bacterial community sample from the inhabited island Barrang Lompo, north transect, water column, free-living fraction, 75m distance from island

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community

characteristic: title


text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Bacterial community sample from the inhabited island Barrang Lompo, north transect, water column, free-living fraction, 150m distance from island

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


characteristic: ENA-CHECKLIST

text: public

text: Bacterial community sample from the inhabited island Barrang Lompo, north transect, water column, free-living fraction, 300m distance from island

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

characteristic: organism

text: marine metagenome

text: Bacterial Community


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

text: public

text: Bacterial community sample from the inhabited island Barrang Lompo, north transect, north transect, water column, particle-attached fraction, 25m distance from island

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


characteristic: External Id

characteristic: INSDC center name

text: public

text: Bacterial community sample from the inhabited island Barrang Lompo, water column, north transect, particle-atached fraction, 75m distance from island

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial community sample from the inhabited island Barrang Lompo, water column, north transect, particle-atached fraction, 150m distance from island

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial community sample from the inhabited island Barrang Lompo, water column, north transect, particle-atached fraction, 300m distance from island

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial community sample from the inhabited island Barrang Lompo, north transect, sediment sample, 25m distance from island

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial community sample from the inhabited island Barrang Lompo, north transect, sediment sample, 75m distance from island

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial community sample from the inhabited island Barrang Lompo, north transect, sediment sample, 150m distance from island

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial community sample from the inhabited island Barrang Lompo, north transect, sediment sample, 300m distance from island

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial community sample from the inhabited island Barrang Lompo, south transect, water column, free-living fraction, 25m distance from island

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial community sample from the inhabited island Barrang Lompo, south transect, water column, free-living fraction, 75m distance from island

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial community sample from the inhabited island Barrang Lompo, south transect, water column, free-living fraction, 150m distance from island

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial community sample from the inhabited island Barrang Lompo, south transect, water column, free-living fraction, 300m distance from island

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial community sample from the inhabited island Barrang Lompo, south transect, south transect, water column, particle-attached fraction, 25m distance from island

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: LEIBNIZ CENTRE FOR TROPICAL MARINE ECOLOGY

text: public

text: Bacterial community sample from the inhabited island Barrang Lompo, water column, south transect, particle-atached fraction, 75m distance from island

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial community sample from the inhabited island Barrang Lompo, water column, south transect, particle-atached fraction, 150m distance from island

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial community sample from the inhabited island Barrang Lompo, water column, south transect, particle-atached fraction, 300m distance from island

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial community sample from the inhabited island Barrang Lompo, south transect, sediment sample, 25m distance from island

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial community sample from the inhabited island Barrang Lompo, south transect, sediment sample, 75m distance from island

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial community sample from the inhabited island Barrang Lompo, south transect, sediment sample, 150m distance from island

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial community sample from the inhabited island Barrang Lompo, south transect, sediment sample, 300m distance from island

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial community sample from the uninhabited island Kodingareng Keke, north transect, water column, free-living fraction, 25m distance from island

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial community sample from the uninhabited island Kodingareng Keke, north transect, water column, free-living fraction, 75m distance from island

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial community sample from the uninhabited island Kodingareng Keke, north transect, water column, free-living fraction, 150m distance from island

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial community sample from the uninhabited island Kodingareng Keke, north transect, water column, free-living fraction, 300m distance from island

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial community sample from the uninhabited island Kodingareng Keke, north transect, north transect, water column, particle-attached fraction, 25m distance from island

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial community sample from the uninhabited island Kodingareng Keke, water column, north transect, particle-atached fraction, 75m distance from island

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial community sample from the uninhabited island Kodingareng Keke, water column, north transect, particle-atached fraction, 150m distance from island

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial community sample from the uninhabited island Kodingareng Keke, water column, north transect, particle-atached fraction, 300m distance from island

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial community sample from the uninhabited island Kodingareng Keke, north transect, sediment sample, 25m distance from island

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: LEIBNIZ CENTRE FOR TROPICAL MARINE ECOLOGY

text: public

text: Bacterial community sample from the uninhabited island Kodingareng Keke, north transect, sediment sample, 75m distance from island

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial community sample from the uninhabited island Kodingareng Keke, north transect, sediment sample, 150m distance from island

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial community sample from the uninhabited island Kodingareng Keke, north transect, sediment sample, 300m distance from island

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial community sample from the uninhabited island Kodingareng Keke, south transect, water column, free-living fraction, 25m distance from island

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial community sample from the uninhabited island Kodingareng Keke, south transect, water column, free-living fraction, 75m distance from island

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial community sample from the uninhabited island Kodingareng Keke, south transect, water column, free-living fraction, 150m distance from island

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial community sample from the uninhabited island Kodingareng Keke, south transect, water column, free-living fraction, 300m distance from island

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial community sample from the uninhabited island Kodingareng Keke, south transect, south transect, water column, particle-attached fraction, 25m distance from island

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial community sample from the uninhabited island Kodingareng Keke, water column, south transect, particle-atached fraction, 75m distance from island

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial community sample from the uninhabited island Kodingareng Keke, water column, south transect, particle-atached fraction, 150m distance from island

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial community sample from the uninhabited island Kodingareng Keke, water column, south transect, particle-atached fraction, 300m distance from island

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial community sample from the uninhabited island Kodingareng Keke, south transect, sediment sample, 25m distance from island

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial community sample from the uninhabited island Kodingareng Keke, south transect, sediment sample, 75m distance from island

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial community sample from the uninhabited island Kodingareng Keke, south transect, sediment sample, 150m distance from island

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial community sample from the uninhabited island Kodingareng Keke, south transect, sediment sample, 300m distance from island

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial biofilm sample from ceramic tile at Badi, 19 km from Makassar, frame III, lower tile facing down, 2 weeks submerged

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: LEIBNIZ CENTRE FOR TROPICAL MARINE ECOLOGY

text: public

text: Bacterial biofilm sample from ceramic tile at Badi, 19 km from Makassar, frame III, lower tile facing down, 4 weeks submerged

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial biofilm sample from ceramic tile at Badi, 19 km from Makassar, frame III, lower tile facing up, 4 weeks submerged

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial biofilm sample from ceramic tile at Badi, 19 km from Makassar, frame III, upper tile facing down, 4 weeks submerged

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial biofilm sample from ceramic tile at Badi, 19 km from Makassar, frame III, lower tile facing down, 6 weeks submerged

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial biofilm sample from ceramic tile at Badi, 19 km from Makassar, frame I, lower tile facing down, 8 weeks submerged

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial biofilm sample from ceramic tile at Badi, 19 km from Makassar, frame I, lower tile facing up, 8 weeks submerged

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial biofilm sample from ceramic tile at Badi, 19 km from Makassar, frame I, upper tile facing down, 8 weeks submerged

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial biofilm sample from ceramic tile at Badi, 19 km from Makassar, frame II, lower tile facing down, 8 weeks submerged

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial biofilm sample from ceramic tile at Badi, 19 km from Makassar, frame II, lower tile facing up, 8 weeks submerged

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial biofilm sample from ceramic tile at Badi, 19 km from Makassar, frame II, upper tile facing up, 8 weeks submerged

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial biofilm sample from ceramic tile at Badi, 19 km from Makassar, frame II, upper tile facing down, 8 weeks submerged

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial biofilm sample from ceramic tile at Badi, 19 km from Makassar, frame III, lower tile facing down, 8 weeks submerged

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial biofilm sample from ceramic tile at Badi, 19 km from Makassar, frame III, lower tile facing up, 8 weeks submerged

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial biofilm sample from ceramic tile at Badi, 19 km from Makassar, frame III, upper tile facing up, 8 weeks submerged

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial biofilm sample from ceramic tile at Badi, 19 km from Makassar, frame III, upper tile facing down, 8 weeks submerged

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial biofilm sample from ceramic tile at Barrang Lompo, 11 km from Makassar, frame III, lower tile facing down, 2 weeks submerged

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: LEIBNIZ CENTRE FOR TROPICAL MARINE ECOLOGY

text: public

text: Bacterial biofilm sample from ceramic tile at Barrang Lompo, 11 km from Makassar, frame III, lower tile facing down, 4 weeks submerged

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial biofilm sample from ceramic tile at Barrang Lompo, 11 km from Makassar, frame III, lower tile facing up, 4 weeks submerged

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial biofilm sample from ceramic tile at Barrang Lompo, 11 km from Makassar, frame III, upper tile facing up, 4 weeks submerged

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial biofilm sample from ceramic tile at Barrang Lompo, 11 km from Makassar, frame III, upper tile facing down, 4 weeks submerged

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial biofilm sample from ceramic tile at Barrang Lompo, 11 km from Makassar, frame III, lower tile facing down, 6 weeks submerged

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial biofilm sample from ceramic tile at Barrang Lompo, 11 km from Makassar, frame I, lower tile facing down, 8 weeks submerged

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial biofilm sample from ceramic tile at Barrang Lompo, 11 km from Makassar, frame I, lower tile facing up, 8 weeks submerged

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial biofilm sample from ceramic tile at Barrang Lompo, 11 km from Makassar, frame I, upper tile facing down, 8 weeks submerged

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial biofilm sample from ceramic tile at Barrang Lompo, 11 km from Makassar, frame II, lower tile facing down, 8 weeks submerged

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial biofilm sample from ceramic tile at Barrang Lompo, 11 km from Makassar, frame II, lower tile facing up, 8 weeks submerged

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial biofilm sample from ceramic tile at Barrang Lompo, 11 km from Makassar, frame II, upper tile facing down, 8 weeks submerged

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial biofilm sample from ceramic tile at Barrang Lompo, 11 km from Makassar, frame III, lower tile facing down, 8 weeks submerged

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial biofilm sample from ceramic tile at Barrang Lompo, 11 km from Makassar, frame III, upper tile facing up, 8 weeks submerged

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial biofilm sample from ceramic tile at Barrang Lompo, 11 km from Makassar, frame III, upper tile facing down, 8 weeks submerged

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial biofilm sample from ceramic tile at Lae Lae, 1 km from Makassar, frame III, lower tile facing down, 2 weeks submerged

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial biofilm sample from ceramic tile at Lae Lae, 1 km from Makassar, frame III, lower tile facing down, 4 weeks submerged

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: LEIBNIZ CENTRE FOR TROPICAL MARINE ECOLOGY

text: public

text: Bacterial biofilm sample from ceramic tile at Lae Lae, 1 km from Makassar, frame III, lower tile facing up, 4 weeks submerged

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial biofilm sample from ceramic tile at Lae Lae, 1 km from Makassar, frame III, upper tile facing up, 4 weeks submerged

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial biofilm sample from ceramic tile at Lae Lae, 1 km from Makassar, frame III, upper tile facing down, 4 weeks submerged

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial biofilm sample from ceramic tile at Lae Lae, 1 km from Makassar, frame III, lower tile facing down, 6 weeks submerged

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial biofilm sample from ceramic tile at Lae Lae, 1 km from Makassar, frame I, lower tile facing down, 8 weeks submerged

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial biofilm sample from ceramic tile at Lae Lae, 1 km from Makassar, frame I, lower tile facing up, 8 weeks submerged

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial biofilm sample from ceramic tile at Lae Lae, 1 km from Makassar, frame I, upper tile facing down, 8 weeks submerged

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial biofilm sample from ceramic tile at Lae Lae, 1 km from Makassar, frame II, lower tile facing down, 8 weeks submerged

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial biofilm sample from ceramic tile at Lae Lae, 1 km from Makassar, frame II, lower tile facing up, 8 weeks submerged

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial biofilm sample from ceramic tile at Lae Lae, 1 km from Makassar, frame II, upper tile facing up, 8 weeks submerged

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial biofilm sample from ceramic tile at Lae Lae, 1 km from Makassar, frame II, upper tile facing down, 8 weeks submerged

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial biofilm sample from ceramic tile at Lae Lae, 1 km from Makassar, frame III, lower tile facing down, 8 weeks submerged

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial biofilm sample from ceramic tile at Lae Lae, 1 km from Makassar, frame III, lower tile facing up, 8 weeks submerged

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial biofilm sample from ceramic tile at Lae Lae, 1 km from Makassar, frame III, upper tile facing up, 8 weeks submerged

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial biofilm sample from ceramic tile at Lae Lae, 1 km from Makassar, frame III, upper tile facing down, 8 weeks submerged

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial biofilm sample from natural surface at Badi, 19 km from Makassar, Transect I

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: LEIBNIZ CENTRE FOR TROPICAL MARINE ECOLOGY

text: public

text: Bacterial biofilm sample from natural surface at Badi, 19 km from Makassar, Transect II

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial biofilm sample from natural surface at Badi, 19 km from Makassar, Transect III

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial biofilm sample from natural surface at Barrang Lompo, 11 km from Makassar, Transect I

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial biofilm sample from natural surface at Barrang Lompo, 11 km from Makassar, Transect II

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial biofilm sample from natural surface at Barrang Lompo, 11 km from Makassar, Transect III

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial biofilm sample from natural surface at Lae Lae, 1 km from Makassar, Transect I

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial biofilm sample from natural surface at Lae Lae, 1 km from Makassar, Transect II

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: public

text: Bacterial biofilm sample from natural surface at Lae Lae, 1 km from Makassar, Transect III

text: Yes

text: Indonesia

text: Spermonde Archipelago

text: marine metagenome

text: marine metagenome

text: Bacterial Community


text: GlaxoSmithKline

text: public

text: ArrayExpress

characteristic: broker name

text: Control

characteristic: comment

text: house mouse

characteristic: common name

text: Protocols: Acute dose screens (3 day). All animal studies were ethically reviewed and carried out in accordance with Animals (Scientific Procedures) Act 1986 and the GSK Policy on the Care, Welfare and Treatment of Animals. Male Crl:CD1(ICR) mice were obtained from Charles River UK Ltd, Margate, Kent. For both acute studies, adult, \wild type\ male CD1 mice were intravenously dosed with 100 mg/kg of each of the ASOs on day 1. The exception was ‘Sequence 1’, which was only administrated at 30 mg/kg due to concerns over its tolerability at a higher dose. A dose of 100 mg/kg was selected as it was below the total cumulative dose that was administrated in the 15 day repeat dose study, but still anticipated to be sufficient for the more hepatotoxic ASOs to cause ALT and AST increases. Four to five mice per group were dosed on day 1 (5 mL/kg), with ASOs formulated in 1x PBS (pH 7.4) at a 20 mg/mL and 6 mg/mL, for 100 mg/kg and 30 mg/kg doses respectively. Total RNA was extracted from ~50 mg of snap-frozen liver using RNeasy Plus Mini Kit (Qiagen)(starting with 800 µL of Buffer RLT Plus and extracting the RNA from 350 µL of lysate) and MagNA Lyser Green Beads (Roche)(tissues were shredded for 33 sec at 6200 rpm). Samples were then quantified and their integrity assessed using Qubit RNA Broad Range Assay Kit (Thermo Fisher Scientific) and an Agilent 2100 Bioanalyzer RNA 6000 Nano Kit (Agilent Technologies) respectively. The extracted RNA had an average RNA integrity number (RIN) score of 8.9 and no sample with a RIN score < 8.1 was used for sequencing. RNA-Seq libraries were prepared with a TruSeq® Stranded Total RNA LT - (with Ribo-Zero™ Human/Mouse/Rat) - Set A kit (Illumina). The library preparation followed the manufacturer’s manual except for two modifications made to the thermal cycler parameters. First, the time of ‘Elution 2 - Frag - Prime’ program was reduced from 8 to 6 min to increase the length of the RNA fragments. Secondly, the number of cycles (11 instead of 15) and the time of the initial step in each cycle (30 instead of 10 sec at the first 98°C step) were both modified to minimise PCR-based amplification bias (‘PCR’ program). Three rounds of qPCR quantification of the final libraries were performed with the KAPA Library Quantification Kit (KAPA Biosystems) on a QuantStudio 12K Flex Real-Time PCR System (Thermo Fisher Scientific).

text: Mus musculus

text: Mus musculus

text: liver

characteristic: organism part

text: acute dosing

characteristic: protocol

text: Mus musculus

text: male

characteristic: sex

text: CD1 mus strain

characteristic: strain

type: has member


text: GTCTCTTTACCTGG

characteristic: ASO sequence

text: GlaxoSmithKline

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: LNA number

text: ArrayExpress

text: Known Toxic

text: house mouse

characteristic: description

text: Mus musculus

text: Mus musculus

text: liver

text: acute dosing

text: Mus musculus

text: male

text: CD1 mus strain

characteristic: title


text: AGTGTGATGAAAAGCA

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: GlaxoSmithKline

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: house mouse

text: Mus musculus

characteristic: organism

text: Mus musculus

text: liver

text: acute dosing

text: Mus musculus

text: male

text: CD1 mus strain


text: AGATGCAACACTACTA

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: GlaxoSmithKline

characteristic: INSDC center name

text: public

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

text: Mus musculus

text: Mus musculus

text: liver

text: acute dosing

text: Mus musculus

text: male

text: CD1 mus strain


text: TGAGAAGATCCATAGC

characteristic: ASO sequence

text: GlaxoSmithKline

text: public

text: ArrayExpress

text: house mouse

text: Mus musculus

text: Mus musculus

text: liver

characteristic: organism part

text: acute dosing

characteristic: protocol

text: Mus musculus

text: male

characteristic: sex

text: CD1 mus strain

characteristic: strain


text: GAGTTATTACTAGAGT

text: GlaxoSmithKline

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: LNA number

text: ArrayExpress

text: house mouse

characteristic: description

text: Mus musculus

text: Mus musculus

text: liver

text: acute dosing

text: Mus musculus

text: male

text: CD1 mus strain

characteristic: title


text: GGAACCAGATGACA

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: GlaxoSmithKline

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: Surrogate

characteristic: comment

text: house mouse

text: Mus musculus

text: Mus musculus

text: liver

text: acute dosing

text: Mus musculus

text: male

text: CD1 mus strain


text: TCAGGGCAGGAATG

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: GlaxoSmithKline

characteristic: INSDC center name

text: public

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

text: Mus musculus

characteristic: organism

text: Mus musculus

text: liver

text: acute dosing

text: Mus musculus

text: male

text: CD1 mus strain


text: GAGTGAAGCAGATC

characteristic: ASO sequence

text: GlaxoSmithKline

characteristic: INSDC first public

text: public

text: ArrayExpress

text: house mouse

text: Mus musculus

text: Mus musculus

text: liver

characteristic: organism part

text: acute dosing

characteristic: protocol

text: Mus musculus

text: male

characteristic: sex

text: CD1 mus strain

characteristic: strain


text: GCAAGTTACTGGGT

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: GlaxoSmithKline

characteristic: INSDC last update

text: public

characteristic: LNA number

text: ArrayExpress

text: house mouse

characteristic: description

text: Mus musculus

text: Mus musculus

text: liver

text: acute dosing

text: Mus musculus

text: male

text: CD1 mus strain

characteristic: title


text: GTCAAATGGAAGCA

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: GlaxoSmithKline

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: ArrayExpress

text: house mouse

text: Mus musculus

text: Mus musculus

text: liver

text: acute dosing

text: Mus musculus

text: male

text: CD1 mus strain


text: GAAGAGTAAGAGCT

characteristic: ASO sequence

text: GlaxoSmithKline

characteristic: INSDC center name

text: public

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

text: Mus musculus

characteristic: organism

text: Mus musculus

text: liver

text: acute dosing

text: Mus musculus

text: male

text: CD1 mus strain


text: AGTGACAATGAGTT

text: GlaxoSmithKline

characteristic: INSDC first public

text: public

text: ArrayExpress

text: house mouse

text: Mus musculus

text: Mus musculus

text: liver

characteristic: organism part

text: acute dosing

characteristic: protocol

text: Mus musculus

text: male

characteristic: sex

text: CD1 mus strain

characteristic: strain


text: TTTGATGAAGTGGT

characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: GlaxoSmithKline

characteristic: INSDC last update

text: public

characteristic: LNA number

text: ArrayExpress

text: house mouse

characteristic: description

text: Mus musculus

text: Mus musculus

text: liver

text: acute dosing

text: Mus musculus

text: male

text: CD1 mus strain


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: GlaxoSmithKline

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

text: Control

characteristic: comment

text: house mouse

text: Protocols: Repeat dose study (15 day). All animal studies were ethically reviewed and carried out in accordance with Animals (Scientific Procedures) Act 1986 and the GSK Policy on the Care, Welfare and Treatment of Animals. Male Crl:CD1(ICR) mice were obtained from Charles River UK Ltd, Margate, Kent. Adult, \wild type\, male CD1 mice were dosed on 5 separate occasions, twice weekly over a period of 15 days. A dose of 30 mg/kg was chosen based on literate review of two key publications available at the time the work was conducted (Swayze et al. 2007; Koch and Ørum 2008), which showed that such a dose should be sufficient to characterise the profile of liver toxicity for the chemistry. Up to 8 mice per group were dosed on days 1, 4, 8, 11 and 15 (5 mL/kg) with the ASOs formulated in 1x PBS (pH 7.4) at 6 mg/mL Total RNA was extracted from ~50 mg of snap-frozen liver using RNeasy Plus Mini Kit (Qiagen)(starting with 800 µL of Buffer RLT Plus and extracting the RNA from 350 µL of lysate) and MagNA Lyser Green Beads (Roche)(tissues were shredded for 33 sec at 6200 rpm). Samples were then quantified and their integrity assessed using Qubit RNA Broad Range Assay Kit (Thermo Fisher Scientific) and an Agilent 2100 Bioanalyzer RNA 6000 Nano Kit (Agilent Technologies) respectively. The extracted RNA had an average RNA integrity number (RIN) score of 8.9 and no sample with a RIN score < 8.1 was used for sequencing. RNA-Seq libraries were prepared with a TruSeq® Stranded Total RNA LT - (with Ribo-Zero™ Human/Mouse/Rat) - Set A kit (Illumina). The library preparation followed the manufacturer’s manual except for two modifications made to the thermal cycler parameters. First, the time of ‘Elution 2 - Frag - Prime’ program was reduced from 8 to 6 min to increase the length of the RNA fragments. Secondly, the number of cycles (11 instead of 15) and the time of the initial step in each cycle (30 instead of 10 sec at the first 98°C step) were both modified to minimise PCR-based amplification bias (‘PCR’ program). Three rounds of qPCR quantification of the final libraries were performed with the KAPA Library Quantification Kit (KAPA Biosystems) on a QuantStudio 12K Flex Real-Time PCR System (Thermo Fisher Scientific).

text: Mus musculus

text: Mus musculus

text: liver

text: repeat dosing

text: Mus musculus

text: male

text: CD1 mus strain

characteristic: title


text: AGTGTGATGAAAAGCA

characteristic: ASO sequence

text: GlaxoSmithKline

characteristic: INSDC center name

text: public

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

text: Mus musculus

characteristic: organism

text: Mus musculus

text: liver

text: repeat dosing

text: Mus musculus

text: male

text: CD1 mus strain


text: TGAGAAGATCCATAGC

characteristic: ENA-CHECKLIST

text: GlaxoSmithKline

characteristic: INSDC first public

text: public

text: ArrayExpress

text: house mouse

text: Mus musculus

text: Mus musculus

text: liver

characteristic: organism part

text: repeat dosing

characteristic: protocol

text: Mus musculus

text: male

characteristic: sex

text: CD1 mus strain

characteristic: strain


text: AGATGCAACACTACTA

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: GlaxoSmithKline

characteristic: INSDC last update

text: public

characteristic: LNA number

text: ArrayExpress

text: house mouse

characteristic: description

text: Mus musculus

text: Mus musculus

text: liver

text: repeat dosing

text: Mus musculus

text: male

text: CD1 mus strain


text: GAGTTATTACTAGAGT

characteristic: ASO sequence

text: GlaxoSmithKline

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

text: ArrayExpress

text: house mouse

text: Mus musculus

text: Mus musculus

text: liver

text: repeat dosing

text: Mus musculus

text: male

text: CD1 mus strain

characteristic: title


E-MTAB-5426:multispecies 1st repeat

characteristic: ENA-CHECKLIST

text: public

text: E-MTAB-5426:multispecies 1st repeat

tag: Namespace:Lab of Pharmaceutical Microbiology - Ghent University - Ghent - Belgium

characteristic: Submitter Id

text: ArrayExpress

characteristic: broker name

text: Protocols: S. anginosus LMG 14502 isolated from a human throat, P. aeruginosa DK2 is a CF isolate, S. aureus LMG 10147 is a human wound isolate S. anginosus LMG 14502 (throat isolate), P. aeruginosa DK2 (CF isolate), and S. aureus LMG 10147 (wound isolate) were cultured overnight at 37°C in Brain Heart Infusion broth (BHI) (Oxoid, Basingstoke, UK). Inoculum suspensions containing S. anginosus alone, or in combination with S. aureus and P. aeruginosa, were made in medium. 96-well microtiter plates were filled with the inoculum suspensions. After 4 hours of adhesion at 37°C, the supernatant was discarded and 100 µl fresh medium was added. Cells were allowed to grow for an additional 20 hours to form a mature biofilm. For each condition 3 independent samples were incubated. RNA was extracted immediately following harvesting of the biofilm cells via vigorously pipetting up and down, using the Ambion RiboPure Bacteria Kit (Ambion, Austin, TX) according to the manufacturer’s instructions. rRNA depletion (on 2 µg total RNA) was done using the Ribo-Zero Magnetic Kit Bacteria (Epicentre, Illumina). Truseq stranded RNA library preparation kit (Illumina) was then used to create strand specific libraries. Quality control of the libraries (DNA 1000 chip, Agilent Technologies, Santa Clara, US) and a concentration assay (qPCR) was performed according recommendations provided by Illumina. Quality control of the libraries (DNA 1000 chip, Agilent Technologies, Santa Clara, US) and a concentration assay (qPCR) was performed according recommendations provided by Illumina.

text: multispecies

characteristic: growth condition

text: Streptococcus anginosus

characteristic: organism

text: Streptococcus anginosus

text: Streptococcus anginosus

text: multispecies 1st repeat


E-MTAB-5426:multispecies 2nd repeat

text: Lab of Pharmaceutical Microbiology - Ghent University - Ghent - Belgium

characteristic: INSDC first public

text: public

text: E-MTAB-5426:multispecies 2nd repeat

text: ArrayExpress

text: multispecies

text: Streptococcus anginosus

text: Streptococcus anginosus

text: Streptococcus anginosus

text: multispecies 2nd repeat


E-MTAB-5426:multispecies 3rd repeat

characteristic: ENA-FIRST-PUBLIC

text: public

text: E-MTAB-5426:multispecies 3rd repeat

text: ArrayExpress

text: multispecies

text: Streptococcus anginosus

text: Streptococcus anginosus

text: Streptococcus anginosus

text: multispecies 3rd repeat


name: E-MTAB-5426:S. anginosus monospecies 1st repeat

characteristic: ENA-LAST-UPDATE

text: public

text: ArrayExpress

text: monospecies

text: Streptococcus anginosus

text: Streptococcus anginosus

text: Streptococcus anginosus

text: S. anginosus monospecies 1st repeat


name: E-MTAB-5426:S. anginosus monospecies 2nd repeat

characteristic: External Id

text: public

text: ArrayExpress

text: monospecies

text: Streptococcus anginosus

text: Streptococcus anginosus

text: Streptococcus anginosus

text: S. anginosus monospecies 2nd repeat


name: E-MTAB-5426:S. anginosus monospecies 3rd repeat

characteristic: INSDC last update

text: public

text: ArrayExpress

text: monospecies

text: Streptococcus anginosus

text: Streptococcus anginosus

text: Streptococcus anginosus

text: S. anginosus monospecies 3rd repeat


text: Animal and Plant Health Agency

text: public

tag: Namespace:Animal and Plant Health Agency

characteristic: collection date

text: Moraxella pluranimalium-like

characteristic: common name

text: United Kingdom

characteristic: geographic location (country and/or sea)

text: healthy

characteristic: host health state

text: porcine

characteristic: host scientific name

characteristic: isolate

text: caecal contents

characteristic: isolation source

text: Moraxella sp. MSG47-C17

text: Moraxella sp. MSG47-C17

text: mcr-1 and mcr-2 variant genes identified in Moraxella species isolated from pigs.


characteristic: ENA-CHECKLIST

text: Animal and Plant Health Agency

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Moraxella porci-like

text: United Kingdom

text: healthy

text: porcine

text: caecal contents

text: Moraxella sp. MSG13-C03

text: Moraxella sp. MSG13-C03

characteristic: title


text: MPICE

characteristic: INSDC first public

text: public

characteristic: Submitter Id

text: Hermetia illucens RNAseq data from larvae exposed to control diet

characteristic: description

text: larva

characteristic: dev stage

text: Hermetia illucens

characteristic: organism

text: Hermetia illucens

text: whole larva

characteristic: tissue lib

text: Hermetia illucens larval transcriptome


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: MPICE

characteristic: INSDC last update

text: public

text: Hermetia illucens RNAseq data from larvae exposed to chitin-rich diet

text: larva

text: Hermetia illucens

text: Hermetia illucens

text: whole larva

text: Hermetia illucens larval transcriptome


text: MPICE

text: public

text: Hermetia illucens RNAseq data from larvae exposed to cellulose-rich diet

text: larva

text: Hermetia illucens

text: Hermetia illucens

text: whole larva

text: Hermetia illucens larval transcriptome


text: MPICE

text: public

text: Hermetia illucens RNAseq data from larvae exposed to lignin-rich diet

text: larva

text: Hermetia illucens

text: Hermetia illucens

text: whole larva

text: Hermetia illucens larval transcriptome


text: MPICE

text: public

text: Hermetia illucens RNAseq data from larvae exposed to protein-rich diet

text: larva

text: Hermetia illucens

text: Hermetia illucens

text: whole larva

text: Hermetia illucens larval transcriptome


text: MPICE

text: public

text: Hermetia illucens RNAseq data from larvae exposed to oil-rich diet

text: larva

text: Hermetia illucens

text: Hermetia illucens

text: whole larva

text: Hermetia illucens larval transcriptome


text: MPICE

text: public

text: Hermetia illucens RNAseq data from larvae exposed to bacteria-rich diet

text: larva

text: Hermetia illucens

text: Hermetia illucens

text: whole larva

text: Hermetia illucens larval transcriptome


name: E-MTAB-5429:Sample1_BLUE1: JS-II-124 Fc1 1-5

text: public

tag: Namespace:European Molecular Biology Laboratory (Hamburg Outstation)

text: ArrayExpress

characteristic: broker name

text: Protocols: collection of material from lab-grown phytoplankton cultures growth protocol for laboratory phytoplankton cultures treatment with experimental factor blue light total RNA extraction with thiocyanate-phenol-chloroform extraction protocol Illumina RNA-seq Sample Prep Kit

text: blue light

characteristic: growth condition

text: Fragilariopsis cylindrus

text: Fragilariopsis cylindrus

text: Fragilariopsis cylindrus

characteristic: strain

text: Sample1_BLUE1: JS-II-124 Fc1 1-5


name: E-MTAB-5429:Sample2_BLUE2: JS-II-124 Fc2 2-5

characteristic: ENA-CHECKLIST

text: European Molecular Biology Laboratory (Hamburg Outstation)

characteristic: INSDC center name

text: public

text: ArrayExpress

text: blue light

text: Fragilariopsis cylindrus

text: Fragilariopsis cylindrus

text: Fragilariopsis cylindrus

text: Sample2_BLUE2: JS-II-124 Fc2 2-5


text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-5429:Sample2_BLUE2: JS-II-124 Fc2 2-5

text: ArrayExpress

text: blue light

text: Fragilariopsis cylindrus

text: Fragilariopsis cylindrus

text: Fragilariopsis cylindrus

text: Sample2_BLUE2: JS-II-124 Fc2 2-5


name: E-MTAB-5429:Sample3_BLUE3: JS-II-124 Fc2 3-5

characteristic: INSDC first public

text: public

text: ArrayExpress

text: blue light

text: Fragilariopsis cylindrus

text: Fragilariopsis cylindrus

text: Fragilariopsis cylindrus

text: Sample3_BLUE3: JS-II-124 Fc2 3-5

characteristic: title


name: E-MTAB-5429:Sample1_RED1: JS-II-122 Fc1 1-1

text: public

characteristic: Submitter Id

text: ArrayExpress

text: Protocols: collection of material from lab-grown phytoplankton cultures growth protocol for laboratory phytoplankton cultures treatment with experimental factor red light total RNA extraction with thiocyanate-phenol-chloroform extraction protocol Illumina RNA-seq Sample Prep Kit

characteristic: description

text: red light

text: Fragilariopsis cylindrus

text: Fragilariopsis cylindrus

text: Fragilariopsis cylindrus

text: Sample1_RED1: JS-II-122 Fc1 1-1


name: E-MTAB-5429:Sample2_RED2: JS-II-122 Fc2 2-1

text: public

text: ArrayExpress

text: red light

text: Fragilariopsis cylindrus

characteristic: organism

text: Fragilariopsis cylindrus

text: Fragilariopsis cylindrus

text: Sample2_RED2: JS-II-122 Fc2 2-1


name: E-MTAB-5429:Sample3_RED3: JS-II-122 Fc3 3-1

text: public

text: ArrayExpress

text: red light

text: Fragilariopsis cylindrus

text: Fragilariopsis cylindrus

text: Fragilariopsis cylindrus

text: Sample3_RED3: JS-II-122 Fc3 3-1


text: public

text: E-MTAB-5429:Sample1_Si1: JS-II-109 Fc1 1-1

text: ArrayExpress

text: Protocols: collection of material from lab-grown phytoplankton cultures growth protocol for laboratory phytoplankton cultures treatment with experimental factor silicate limitation total RNA extraction with thiocyanate-phenol-chloroform extraction protocol Illumina RNA-seq Sample Prep Kit

text: silicate limitation

text: Fragilariopsis cylindrus

text: Fragilariopsis cylindrus

text: Fragilariopsis cylindrus

text: Sample1_Si1: JS-II-109 Fc1 1-1


text: public

text: E-MTAB-5429:Sample2_Si2: JS-II-109 Fc2 2-1

text: ArrayExpress

text: silicate limitation

text: Fragilariopsis cylindrus

text: Fragilariopsis cylindrus

text: Fragilariopsis cylindrus

text: Sample2_Si2: JS-II-109 Fc2 2-1


text: public

text: E-MTAB-5429:Sample3_Si3: JS-II-109 Fc3 3-1

text: ArrayExpress

text: silicate limitation

text: Fragilariopsis cylindrus

text: Fragilariopsis cylindrus

text: Fragilariopsis cylindrus

text: Sample3_Si3: JS-II-109 Fc3 3-1


text: University Hospital Galway

text: public

tag: Namespace:University Hospital Galway

characteristic: collection date

text: Ireland

characteristic: geographic location (country and/or sea)

text: Homo sapiens

characteristic: host scientific name

characteristic: isolate

text: Human

characteristic: isolation source

text: Salmonella enterica subsp. enterica serovar Paratyphi A

text: Paratyphi A

characteristic: serovar

text: Subsp. I

characteristic: sub species


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University Hospital Galway

characteristic: INSDC last update

text: public

text: Ireland

text: Homo sapiens

text: Human

text: Salmonella enterica subsp. Enterica

text: Salmonella enterica subsp. Enterica

text: Unnamed

text: Subsp. I


characteristic: ENA-CHECKLIST

text: University Hospital Galway

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Ireland

text: Homo sapiens

text: Human

text: Salmonella enterica subsp. enterica serovar Newport

text: Newport

text: Subsp. I


text: University Hospital Galway

characteristic: INSDC first public

text: public

characteristic: Submitter Id

text: Ireland

text: Homo sapiens

text: Human

text: Salmonella enterica subsp. enterica serovar Infantis

text: Infantis

text: Subsp. I

characteristic: title


text: University Hospital Galway

text: public

characteristic: collection date

text: Ireland

characteristic: geographic location (country and/or sea)

text: Homo sapiens

text: Human

text: Salmonella enterica subsp. enterica serovar Typhimurium var. monophasic 4,[5],12:i:-

characteristic: organism

text: Subsp. I


text: University Hospital Galway

text: public

text: Ireland

text: Homo sapiens

characteristic: host scientific name

characteristic: isolate

text: Human

characteristic: isolation source

text: Salmonella enterica subsp. enterica serovar Enteritidis

text: Enteritidis

text: Subsp. I


characteristic: ENA-FIRST-PUBLIC

text: University Hospital Galway

text: public

text: Ireland

text: Homo sapiens

text: Human

text: Salmonella enterica subsp. enterica serovar Java

text: Java

characteristic: serovar

text: Subsp. I

characteristic: sub species


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: APHA

characteristic: INSDC last update

text: public

text: United Kingdom

text: diseased

characteristic: host health state

text: Gallus gallus

text: Chicken liver

text: Campylobacter hepaticus

text: Campylobacter hepaticus

text: CampySLD sequencing


characteristic: ENA-CHECKLIST

text: UPSC

characteristic: INSDC center name

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Caucausian spruce

characteristic: common name

text: Voucher 19830568 The Arboretum at Horsholm; cultivated from germplasm collected at Ordu Tekmazar Turkey

characteristic: description

text: Picea orientalis

text: Picea orientalis

text: Picea orientalis cultivated at The Arboretum at Horsholm University of Copenhagen Denmark; Voucher 19830568


text: UPSC

characteristic: INSDC first public

text: public

characteristic: Submitter Id

text: Caucausian spruce

text: Voucher 19870081 The Arboretum at Horsholm; cultivated from germplasm collected in Borjomi Georgia

text: Picea orientalis

characteristic: organism

text: Picea orientalis

text: Picea orientalis cultivated at The Arboretum at Horsholm University of Copenhagen Denmark; Voucher 19870081

characteristic: title


characteristic: ENA-FIRST-PUBLIC

text: UPSC

text: public

text: Colorado spruce

text: Voucher 19780256 The Arboretum at Horsholm; cultivated from germplasm collected at White Mountains Arizona USA

text: Picea pungens

text: Picea pungens

text: Picea pungens cultivated at The Arboretum at Horsholm University of Copenhagen Denmark; Voucher 19780256


text: UPSC

text: public

text: Colorado spruce

text: Voucher 19780259 The Arboretum at Horsholm; cultivated from germplasm collected in Buford Colorado USA

text: Picea pungens

text: Picea pungens

text: Picea pungens cultivated at The Arboretum at Horsholm University of Copenhagen Denmark; Voucher 19780259


text: UPSC

text: public

text: Green dragon spruce

text: Voucher 2004 Lanzhou University Botanical Garden

text: Picea retroflexa

text: Picea retroflexa

text: Picea retroflexa collected from a natural population Sichuan China near 31N 101E


text: UPSC

text: public

text: Green dragon spruce

text: Voucher 1872 Lanzhou University Botanical Garden

text: Picea retroflexa

text: Picea retroflexa

text: Picea retroflexa collected from a natural population Sichuan China near 33N 104E


text: UPSC

text: public

text: Red spruce

text: Cultivated from material collected at Mount Pisgah North Carolina USA

text: Picea rubens

text: Picea rubens

text: Picea rubens cultivated at Charles Keith Arboretum USA


text: UPSC

text: public

text: Red spruce

text: Voucher 6169 Rogow Arboretum; cultivated from germplasm from Lac-Edouard Quebec Canada

text: Picea rubens

text: Picea rubens

text: Picea rubens cultivated at Rogow Arboretum Poland


text: UC SANTA CRUZ

text: public

tag: Namespace:UC SANTA CRUZ

text: C. elegans

text: Canu Nanopore R9 assembly

text: Caenorhabditis elegans

text: Caenorhabditis elegans


text: UC SANTA CRUZ

text: public

text: canu_pilon_nanopore_draft_assembly.tar.gz

text: C. elegans

text: Canu Nanopore R9 assembly post Pilon polishing

text: Caenorhabditis elegans

text: Caenorhabditis elegans


text: UC SANTA CRUZ

text: public

text: C. elegans

text: Nanopore R9 FASTA data

text: Caenorhabditis elegans

text: Caenorhabditis elegans


text: UPSC

text: public

text: Picea schrenkiana

text: Voucher 15702 Rogow Arboretum; cultivated from germplasm from Dzungarski Altau Kazakhstan

text: Picea schrenkiana

text: Picea schrenkiana

text: Picea schrenkiana cultivated at Rogow Arboretum Poland


text: UPSC

text: public

text: Picea schrenkiana

text: Voucher 1990060 The Arboretum at Horsholm; cultivated from germplasm collected in Tian Shan Krygyzstan

text: Picea schrenkiana

text: Picea schrenkiana

text: Picea schrenkiana cultivated at The Arboretum at Horsholm University of Copenhagen Denmark; Voucher 1990060


text: UPSC

text: public

text: Picea schrenkiana

text: Voucher 19860197 The Arboretum at Horsholm; cultivated from germplasm collected from Xinjiang China near 44N 89E

text: Picea schrenkiana

text: Picea schrenkiana

text: Picea schrenkiana cultivated at The Arboretum at Horsholm University of Copenhagen Denmark; Voucher 19860197


E-MTAB-5418:Sample 1

text: University of Cambridge

text: public

text: E-MTAB-5418:Sample 1

tag: Namespace:University of Cambridge

text: ArrayExpress

characteristic: broker name

text: Vero E6

characteristic: cell line

text: Protocols: Vero E6 cells and C6/36 cells were maintained in Dulbecco’s modification of Eagle’s medium supplemented with 10% (vol/vol) fetal calf serum (FCS). ZIKV strain Pe243 (ZIKV-PE243) was derived as previously described (Donald et al., 2016). At 24 h p.i., cells were treated with CHX (Sigma-Aldrich; to 100 µg/ml; 2 min). Cells were rinsed with 5 ml of ice-cold PBS, the dishes submerged in a reservoir of liquid nitrogen for 10 s, transferred to dry ice and 400 µl of lysis buffer [20 mM Tris-HCl pH 7.5, 150 mM NaCl, 5 mM MgCl2, 1 mM DTT, 1% Triton X-100, 100 µg/ml cycloheximide and 25 U/ml TURBO™ DNase (Life Technologies)] dripped on. Cells were scraped extensively to ensure lysis, collected and triturated with a 26-G needle ten times. Lysates were clarified by centrifugation for 20 min at 13,000 g at 4 ºC, the supernatants recovered and stored in liquid nitrogen. Cell lysates were subjected to RiboSeq and RNASeq. The methodologies employed were based on the original protocols of Ingolia and colleagues (Ingolia et al., 2009; Ingolia et al., 2012), except ribosomal RNA contamination was removed by treatment with duplex-specific nuclease (DSN) for Samples 7 and 8, and library amplicons were constructed using a small RNA cloning strategy (Guo et al., 2010) adapted to Illumina small RNA v2 to allow multiplexing. The methods used were as described by Irigoyen et al. (2016).

text: ZIKV-PE243 virus

characteristic: infect

text: Chlorocebus sabaeus

text: Chlorocebus sabaeus

text: Chlorocebus sabaeus

text: Ribo-Seq

characteristic: sequencing protocol

unit: hour

characteristic: time point

text: Sample 1


E-MTAB-5418:Sample 10

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Cambridge

characteristic: INSDC last update

text: public

text: E-MTAB-5418:Sample 10

text: ArrayExpress

text: Asian tiger mosquito

text: ZIKV-PE243 virus

text: Aedes albopictus

text: Aedes albopictus

text: Aedes albopictus

text: RNA-Seq

text: Sample 10


E-MTAB-5418:Sample 2

characteristic: ENA-CHECKLIST

text: University of Cambridge

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: E-MTAB-5418:Sample 2

text: ArrayExpress

text: Vero E6

text: ZIKV-PE243 virus

text: Chlorocebus sabaeus

text: Chlorocebus sabaeus

text: Chlorocebus sabaeus

text: Ribo-Seq

text: Sample 2


E-MTAB-5418:Sample 3

text: University of Cambridge

text: public

characteristic: SRA accession

text: E-MTAB-5418:Sample 3

text: ArrayExpress

text: Vero E6

characteristic: description

text: ZIKV-PE243 virus

text: Chlorocebus sabaeus

text: Chlorocebus sabaeus

text: Chlorocebus sabaeus

text: RNA-Seq

text: Sample 3


E-MTAB-5418:Sample 4

text: University of Cambridge

characteristic: INSDC first public

text: public

text: E-MTAB-5418:Sample 4

characteristic: Submitter Id

text: ArrayExpress

text: Vero E6

text: ZIKV-PE243 virus

text: Chlorocebus sabaeus

text: Chlorocebus sabaeus

text: Chlorocebus sabaeus

text: RNA-Seq

text: Sample 4


E-MTAB-5418:Sample 5

text: University of Cambridge

text: public

text: E-MTAB-5418:Sample 5

text: ArrayExpress

text: Asian tiger mosquito

characteristic: common name

text: ZIKV-PE243 virus

text: Aedes albopictus

characteristic: organism

text: Aedes albopictus

text: Aedes albopictus

text: Ribo-Seq

text: Sample 5

characteristic: title


E-MTAB-5418:Sample 6

characteristic: ENA-FIRST-PUBLIC

text: University of Cambridge

text: public

text: E-MTAB-5418:Sample 6

text: ArrayExpress

characteristic: broker name

characteristic: cell line

text: Asian tiger mosquito

text: ZIKV-PE243 virus

text: Aedes albopictus

text: Aedes albopictus

text: Aedes albopictus

text: Ribo-Seq

text: Sample 6


E-MTAB-5418:Sample 7

text: University of Cambridge

text: public

text: E-MTAB-5418:Sample 7

text: ArrayExpress

text: Asian tiger mosquito

text: ZIKV-PE243 virus

characteristic: infect

text: Aedes albopictus

text: Aedes albopictus

text: Aedes albopictus

text: Ribo-Seq

characteristic: sequencing protocol

characteristic: time point

text: Sample 7


E-MTAB-5418:Sample 8

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University of Cambridge

characteristic: INSDC last update

text: public

text: E-MTAB-5418:Sample 8

text: ArrayExpress

text: Asian tiger mosquito

text: ZIKV-PE243 virus

text: Aedes albopictus

text: Aedes albopictus

text: Aedes albopictus

text: Ribo-Seq

text: Sample 8


E-MTAB-5418:Sample 9

characteristic: ENA-CHECKLIST

text: University of Cambridge

characteristic: INSDC center name

text: public

characteristic: INSDC status

text: E-MTAB-5418:Sample 9

text: ArrayExpress

text: Asian tiger mosquito

text: ZIKV-PE243 virus

text: Aedes albopictus

text: Aedes albopictus

text: Aedes albopictus

text: RNA-Seq

text: Sample 9


text: FIOCRUZ-MG

characteristic: INSDC first public

text: public

characteristic: SRA accession

characteristic: collection date

text: metagenome

characteristic: description

text: human-gut

characteristic: environment (biome)

text: intestine

characteristic: environment (feature)

text: feces

characteristic: environment (material)

text: Brazil

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: human-gut

characteristic: human gut environmental package

text: mimarks-survey

characteristic: investigation type

text: metagenome

text: Advento Study

characteristic: project name

text: metagenome

text: Illumina

characteristic: sequencing method


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UPSC

characteristic: INSDC last update

text: public

characteristic: Submitter Id

text: Picea smithiana

characteristic: common name

text: Voucher 19840137 The Arboretum at Horsholm; cultivated from germplasm collected at Naltar Valley Pakistan

text: Picea smithiana

characteristic: organism

text: Picea smithiana

text: Picea smithiana cultivated at The Arboretum at Horsholm University of Copenhagen Denmark; Voucher 19840137

characteristic: title


text: UPSC

characteristic: INSDC center name

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Picea smithiana

text: Voucher 19860408 The Arboretum at Horsholm; cultivated from germplasm collected at Paktia Afghanistan

characteristic: description

text: Picea smithiana

text: Picea smithiana

text: Picea smithiana cultivated at The Arboretum at Horsholm University of Copenhagen Denmark; Voucher 19860408


text: UPSC

text: public

text: Picea spinulosa

text: Voucher F022 Lanzhou University Botanical Garden

text: Picea spinulosa

text: Picea spinulosa

text: Picea spinulosa cultivated at Lazhou University Botanical Garden


text: UPSC

text: public

text: tigertail spruce

text: Voucher 99251086 The Arboretum at Horsholm; cultivated from germplasm collected in Honshu Japan

text: Picea torano

text: Picea torano

text: Picea torano cultivated at The Arboretum at Horsholm University of Copenhagen Denmark


text: UPSC

text: public

text: Picea wilsonii

text: Voucher 19800115 The Arboretum at Horsholm; cultivated from germplasm collected in Luliang Shan Shanxi China

text: Picea wilsonii

text: Picea wilsonii

text: Picea wilsonii cultivated at The Arboretum at Horsholm University of Copenhagen Denmark


text: UPSC

text: public

text: Serbian spruce

text: Voucher 37358

text: Picea omorika

text: Picea omorika

text: Picea omorika cultivated at Royal Botanic Garden Kew UK


text: UPSC

text: public

text: Sitka spruce

text: Voucher 5294; cultivated from germplasm collected in Homer Alaska USA

text: Picea sitchensis

text: Picea sitchensis

text: Picea sitchensis cultivated at Arboretum Norr Umea Sweden


text: UPSC

text: public

text: Sargent spruce

text: Voucher 18159; cultivated from germplasm collected in Sichuan China

text: Picea brachytyla

text: Picea brachytyla

text: Picea brachytyla cultivated at Royal Botanic Garden Kew UK


text: University Hospital Galway

text: public

characteristic: collection date

text: Ireland

characteristic: geographic location (country and/or sea)

text: Homo sapiens

characteristic: host scientific name

characteristic: isolate

text: Human

characteristic: isolation source

text: Salmonella enterica subsp. enterica serovar Typhi

text: Typhi

characteristic: serovar

text: Subsp. I

characteristic: sub species


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University Hospital Galway

characteristic: INSDC last update

text: public

text: Ireland

text: Homo sapiens

text: Human

text: Salmonella enterica subsp. Enterica

text: Salmonella enterica subsp. Enterica

text: Unnamed

text: Subsp. I


text: University Hospital Galway

characteristic: INSDC center name

text: public

characteristic: Submitter Id

text: Ireland

text: Homo sapiens

text: Human

text: Salmonella enterica subsp. enterica serovar Enteritidis

characteristic: organism

text: Enteritidis

text: Subsp. I

characteristic: title


text: University Hospital Galway

characteristic: INSDC first public

text: public

characteristic: INSDC status

characteristic: SRA accession

text: Ireland

text: Homo sapiens

text: Human

text: Salmonella enterica subsp. enterica serovar Bareilly

text: Bareilly

text: Subsp. I


text: University Hospital Galway

text: public

characteristic: collection date

text: Ireland

characteristic: geographic location (country and/or sea)

text: Homo sapiens

characteristic: host scientific name

text: Human

text: Salmonella enterica subsp. enterica serovar Typhimurium

text: Typhimurium

text: Subsp. I


text: University Hospital Galway

text: public

text: Ireland

text: Homo sapiens

characteristic: isolate

text: Human

characteristic: isolation source

text: Salmonella enterica subsp. enterica serovar Corvallis

text: Corvallis

characteristic: serovar

text: Subsp. I


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: University Hospital Galway

text: public

text: Ireland

text: Homo sapiens

text: Human

text: Salmonella enterica subsp. enterica serovar Typhimurium var. monophasic 4,[5],12:i:-

text: Subsp. I

characteristic: sub species


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University Hospital Galway

characteristic: INSDC last update

text: public

text: Ireland

text: Homo sapiens

text: Human

text: Salmonella enterica subsp. enterica serovar Java

text: Java

text: Subsp. I


text: University Hospital Galway

characteristic: INSDC center name

text: public

characteristic: Submitter Id

text: Ireland

text: Homo sapiens

text: Human

text: Salmonella enterica subsp. enterica serovar Kentucky

characteristic: organism

text: Kentucky

text: Subsp. I


text: Staphylococcus aureus

characteristic: ArrayExpress-Species

text: Staphylococcus aureus

characteristic: ArrayExpress-StrainOrLine

text: SC

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: Staphylococcus aureus

characteristic: common name

text: Staphylococcus aureus

text: DNA

characteristic: sample description

text: Staphylococcus aureus

text: Staphylococcus aureus

characteristic: strain

characteristic: title


text: Streptococcus lutetiensis

text: Streptococcus lutetiensis

characteristic: ENA-CHECKLIST

text: SC

text: public

characteristic: SRA accession

text: Streptococcus lutetiensis

text: Streptococcus lutetiensis

text: DNA

text: Streptococcus lutetiensis

text: Streptococcus lutetiensis


text: Streptococcus agalactiae

text: Streptococcus agalactiae

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: Streptococcus agalactiae

text: Streptococcus agalactiae

text: DNA

text: Streptococcus agalactiae

text: Streptococcus agalactiae


text: Streptococcus pneumoniae

text: Streptococcus pneumoniae

characteristic: ENA-LAST-UPDATE

text: SC

text: public

text: Streptococcus pneumoniae

text: Streptococcus pneumoniae

text: DNA

text: Streptococcus pneumoniae

text: Streptococcus pneumoniae


text: Staphylococcus pastueri

text: Staphylococcus pastueri

characteristic: External Id

text: SC

text: public

text: Staphylococcus pastueri

text: Staphylococcus pasteuri

text: DNA

text: Staphylococcus pasteuri

text: Staphylococcus pastueri


text: Sterptococcus gallolyticus

text: Sterptococcus gallolyticus

text: SC

characteristic: INSDC last update

text: public

text: Sterptococcus gallolyticus

text: Streptococcus gallolyticus

text: DNA

text: Streptococcus gallolyticus

text: Sterptococcus gallolyticus


text: Staphyococcus warneri

text: Staphyococcus warneri

text: SC

characteristic: INSDC center name

text: public

text: Staphyococcus warneri

text: Staphylococcus warneri

text: DNA

text: Staphylococcus warneri

text: Staphyococcus warneri


text: Globicatella sulfidifaciens

text: Globicatella sulfidifaciens

text: SC

text: public

characteristic: Submitter Id

text: Globicatella sulfidifaciens

text: Globicatella sulfidifaciens

text: DNA

text: Globicatella sulfidifaciens

text: Globicatella sulfidifaciens


text: Streptococcus pyogenes

text: Streptococcus pyogenes

text: SC

text: public

text: Streptococcus pyogenes

text: Streptococcus pyogenes

characteristic: organism

text: DNA

text: Streptococcus pyogenes

text: Streptococcus pyogenes


text: Streptococus pneumoniae

characteristic: ArrayExpress-Species

text: Streptococus pneumoniae

text: SC

text: public

text: Streptococus pneumoniae

text: Streptococcus pneumoniae

text: DNA

text: Streptococcus pneumoniae

text: Streptococus pneumoniae


text: Oligella urethralis

text: Oligella urethralis

characteristic: ArrayExpress-StrainOrLine

text: SC

text: public

text: Oligella urethralis

text: Oligella urethralis

text: DNA

text: Oligella urethralis

text: Oligella urethralis


text: Staphylococus epidermidis

text: Staphylococus epidermidis

text: SC

characteristic: INSDC first public

text: public

text: Staphylococus epidermidis

text: Staphylococcus epidermidis

text: DNA

text: Staphylococcus epidermidis

text: Staphylococus epidermidis


text: Microbacterium hominis

text: Microbacterium hominis

text: SC

text: public

characteristic: INSDC status

text: Microbacterium hominis

text: Microbacterium hominis

text: DNA

text: Microbacterium hominis

text: Microbacterium hominis


text: Kocuria marina

text: Kocuria marina

text: SC

text: public

text: Kocuria marina

characteristic: common name

text: Kocuria marina

text: DNA

text: Kocuria marina

text: Kocuria marina


text: Myroides odoratimimus

text: Myroides odoratimimus

text: SC

text: public

text: Myroides odoratimimus

text: Myroides odoratimimus

text: DNA

characteristic: sample description

text: Myroides odoratimimus

text: Myroides odoratimimus


text: Steptococcus sanguinis

text: Steptococcus sanguinis

text: SC

text: public

text: Steptococcus sanguinis

text: Streptococcus sanguinis

text: DNA

text: Streptococcus sanguinis

text: Steptococcus sanguinis

characteristic: strain


text: Rhodococcus rhodochrous

text: Rhodococcus rhodochrous

text: SC

text: public

text: Rhodococcus rhodochrous

text: Rhodococcus rhodochrous

text: DNA

text: Rhodococcus rhodochrous

text: Rhodococcus rhodochrous

characteristic: title


characteristic: ENA-CHECKLIST

text: University Hospital Galway

text: public

characteristic: collection date

text: Ireland

characteristic: geographic location (country and/or sea)

text: Homo sapiens

characteristic: host scientific name

characteristic: isolate

text: Human

characteristic: isolation source

text: Typhimurium

characteristic: serovar

text: Subsp. I

characteristic: sub species


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University Hospital Galway

characteristic: INSDC center name

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: Ireland

text: Homo sapiens

text: Human

characteristic: organism

text: Enteritidis

text: Subsp. I


text: University Hospital Galway

characteristic: INSDC first public

text: public

characteristic: INSDC status

text: Ireland

text: Homo sapiens

text: Human

text: Salmonella enterica subsp. enterica serovar Ohio

text: Ohio

text: Subsp. I

characteristic: title


characteristic: ENA-CHECKLIST

text: University Hospital Galway

text: public

characteristic: collection date

text: Ireland

characteristic: geographic location (country and/or sea)

text: Homo sapiens

text: Human

text: Salmonella enterica subsp. Enterica

text: Salmonella enterica subsp. Enterica

text: Unnamed

text: Subsp. I


text: University Hospital Galway

text: public

text: Ireland

text: Homo sapiens

characteristic: host scientific name

characteristic: isolate

text: Human

characteristic: isolation source

text: Salmonella enterica subsp. enterica serovar Mbandaka

text: Mbandaka

text: Subsp. I


characteristic: ENA-FIRST-PUBLIC

text: University Hospital Galway

text: public

text: Ireland

text: Homo sapiens

text: Human

characteristic: serovar

text: Subsp. I

characteristic: sub species


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University Hospital Galway

characteristic: INSDC center name

text: public

text: Ireland

text: Homo sapiens

text: Human

text: Typhi

text: Subsp. I


text: University Hospital Galway

characteristic: INSDC last update

text: public

characteristic: SRA accession

characteristic: Submitter Id

text: Ireland

text: Homo sapiens

text: Human

text: Salmonella enterica subsp. enterica serovar Saintpaul

text: Saintpaul

text: Subsp. I


text: Department of New Biology, DGIST

characteristic: INSDC first public

text: public

characteristic: INSDC status

tag: Namespace:Department of New Biology, DGIST

text: ArrayExpress

characteristic: broker name

text: house mouse

characteristic: common name

text: Protocols: Ovulated oocytes were collected from female mice crossed with vasectomised male at 9am, E0.5, where mating is assumed have occurred at E0.0. 1-cell and 2-cell embryos were collected at 4pm on E0.5 and 9am-3pm on E1.5 respectively. All oocytes and embryos were morphologically assessed to ensure only viable samples are collected. Oocytes were incubated with 0.3 mg/ml hyaluronidase (Sigma, St. Louis, MO) to remove the cumulus cells. Zona pellucida was removed from oocytes and embryos prior to lysis. RNA were extracted from single oocytes, 1-cell embryos or whole 2-cell embryos; and cDNA were amplified as described previously with modifications (Tang et al., 2010). Between 1:10,000 - 1:60,000 dilution of ERCC RNA Spike-In mix (ThermoFisher Scientific, Hemel Hempstead, UK) was added to lysis buffer to estimate efficiency of amplification. Tang, F., Barbacioru, C., Nordman, E., Li, B., Xu, N., Bashkirov, V.I., Lao, K., and Surani, M.A. (2010). RNA-Seq analysis to capture the transcriptome landscape of a single cell. Nat Protoc 5, 516-535. For RNA-seq, cDNA were fragmented to ~ 200bp with Focused-ultrasonicator (Covaris, Woburn, MA) and adaptor ligated libraries were generated using NEBNext® UltraTM DNA library Prep Kit for illumina® (New England Labs, Ipswich, MA).

characteristic: description

text: 2-cell embryo

characteristic: developmental stage

text: wild type genotype

characteristic: genotype

text: Mus musculus

characteristic: organism

text: Mus musculus

text: whole organism

characteristic: organism part

text: Mus musculus

characteristic: strain


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: AIX-MARSEILLE UNIVERSITY

text: public

tag: Namespace:AIX-MARSEILLE UNIVERSITY

text: Complete genome of Mesotoga infera strain VNs100

text: Mesotoga infera

text: Mesotoga infera

characteristic: title


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University Hospital Galway

characteristic: INSDC center name

text: public

characteristic: collection date

text: Ireland

characteristic: geographic location (country and/or sea)

text: Homo sapiens

characteristic: host scientific name

characteristic: isolate

text: Human

characteristic: isolation source

text: Salmonella enterica subsp. enterica serovar Muenster

text: Muenster

characteristic: serovar

text: Subsp. I

characteristic: sub species


text: University Hospital Galway

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Ireland

text: Homo sapiens

text: Human

text: Salmonella enterica subsp. enterica serovar Paratyphi A

text: Paratyphi A

text: Subsp. I


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

text: University Hospital Galway

text: public

text: Ireland

text: Homo sapiens

text: Human

characteristic: organism

text: Java

text: Subsp. I


characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: University Hospital Galway

text: public

text: Ireland

text: Homo sapiens

text: Human

text: Salmonella enterica subsp. enterica serovar Bovismorbificans

text: Bovismorbificans

text: Subsp. I

characteristic: title


text: University Hospital Galway

characteristic: INSDC center name

text: public

characteristic: collection date

text: Ireland

characteristic: geographic location (country and/or sea)

text: Homo sapiens

text: Human

text: Salmonella enterica subsp. enterica serovar Infantis

text: Infantis

text: Subsp. I


text: Staphylococcus condimenti

characteristic: ArrayExpress-Species

text: SC

text: public

text: Staphylococcus condimenti

characteristic: common name

text: Staphylococcus condimenti

text: Staphylococcus condimenti


text: UCSDMI

text: public

characteristic: anonymized name

text: UBERON:oral cavity

characteristic: body habitat

text: UBERON:dental plaque

characteristic: body product

text: UBERON:gingival sulcus

characteristic: body site

text: B

characteristic: bop

characteristic: description

text: A

characteristic: diseasclass

text: TRUE

characteristic: elevation

text: urban biome

characteristic: env biome

text: human-associated habitat

characteristic: env feature

text: human-oral

characteristic: env package

text: organic material

text: USA:CA

characteristic: geo loc name

text: human

characteristic: host common name

text: Homo sapiens

characteristic: host scientific name

characteristic: host subject id

characteristic: host taxid

text: n

characteristic: improve

characteristic: individual

characteristic: inflammation

characteristic: latitude

characteristic: longitude

text: human oral metagenome

text: B

characteristic: pdepthreclass

text: SDSU

text: FALSE

text: A

characteristic: pocketclass

characteristic: pocketdepth

text: dental plaque

characteristic: sample type

text: bx

characteristic: samplesite

text: human oral metagenome

text: disto-buccal

characteristic: time

characteristic: timepoint

text: Meta-omics analysis of periodontal pocket microbial communities pre- and post-treatment

text: upper left first molar

text: T


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: anonymized name

text: UBERON:oral cavity

characteristic: body habitat

text: UBERON:dental plaque

characteristic: body product

text: UBERON:gingival sulcus

characteristic: body site

text: B

characteristic: bop

characteristic: description

text: A

characteristic: diseasclass

text: TRUE

characteristic: elevation

text: urban biome

characteristic: env biome

text: human-associated habitat

characteristic: env feature

text: human-oral

characteristic: env package

text: organic material

text: USA:CA

characteristic: geo loc name

text: human

text: Homo sapiens

text: y

text: human oral metagenome

characteristic: organism

text: B

text: SDSU

text: FALSE

text: A

text: dental plaque

text: by

text: human oral metagenome

text: disto-lingual

characteristic: title

text: upper right first molar

text: T


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: UBERON:oral cavity

text: UBERON:dental plaque

text: UBERON:gingival sulcus

text: N

text: A

text: TRUE

text: urban biome

text: human-associated habitat

text: human-oral

text: organic material

text: USA:CA

text: human

characteristic: host common name

text: Homo sapiens

characteristic: host scientific name

characteristic: host subject id

characteristic: host taxid

text: n

characteristic: improve

characteristic: individual

characteristic: inflammation

characteristic: latitude

characteristic: longitude

text: human oral metagenome

text: A

characteristic: pdepthreclass

text: SDSU

text: FALSE

text: A

characteristic: pocketclass

characteristic: pocketdepth

text: dental plaque

characteristic: sample type

text: bz

characteristic: samplesite

text: human oral metagenome

text: disto-buccal

characteristic: time

characteristic: timepoint

text: lower left canine

text: T


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: anonymized name

text: UBERON:oral cavity

characteristic: body habitat

text: UBERON:dental plaque

characteristic: body product

text: UBERON:gingival sulcus

characteristic: body site

text: Missing: Not provided

characteristic: bop

characteristic: description

text: A

characteristic: diseasclass

text: TRUE

characteristic: elevation

text: urban biome

characteristic: env biome

text: human-associated habitat

characteristic: env feature

text: human-oral

characteristic: env package

text: organic material

text: USA:CA

characteristic: geo loc name

text: human

text: Homo sapiens

text: Missing: Not provided

text: human oral metagenome

characteristic: organism

text: Missing: Not provided

text: SDSU

text: FALSE

text: D

text: Not applicable - suprgingival

text: dental plaque

text: pa

text: human oral metagenome

text: Missing: Not provided

characteristic: title

text: Missing: Not provided

text: Missing: Not provided

text: T


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: UBERON:oral cavity

text: UBERON:dental plaque

text: UBERON:gingival sulcus

text: B

text: A

text: TRUE

text: urban biome

text: human-associated habitat

text: human-oral

text: organic material

text: USA:CA

text: human

characteristic: host common name

text: Homo sapiens

characteristic: host scientific name

characteristic: host subject id

characteristic: host taxid

text: n

characteristic: improve

characteristic: individual

characteristic: inflammation

characteristic: latitude

characteristic: longitude

text: human oral metagenome

text: B

characteristic: pdepthreclass

text: SDSU

text: FALSE

text: A

characteristic: pocketclass

characteristic: pocketdepth

text: dental plaque

characteristic: sample type

text: bx

characteristic: samplesite

text: human oral metagenome

text: disto-buccal

characteristic: time

characteristic: timepoint

text: Meta-omics aMissing: Not providedlysis of periodontal pocket microbial communities pre- and post-treatment

text: upper left first molar

text: T


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: anonymized name

text: UBERON:oral cavity

characteristic: body habitat

text: UBERON:dental plaque

characteristic: body product

text: UBERON:gingival sulcus

characteristic: body site

text: B

characteristic: bop

characteristic: description

text: A

characteristic: diseasclass

text: TRUE

characteristic: elevation

text: urban biome

characteristic: env biome

text: human-associated habitat

characteristic: env feature

text: human-oral

characteristic: env package

text: organic material

text: USA:CA

characteristic: geo loc name

text: human

text: Homo sapiens

text: n

text: human oral metagenome

characteristic: organism

text: A

text: SDSU

text: FALSE

text: A

text: dental plaque

text: bz

text: human oral metagenome

text: disto-buccal

characteristic: title

text: lower left canine

text: T


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: UBERON:oral cavity

text: UBERON:dental plaque

text: UBERON:gingival sulcus

text: B

text: A

text: TRUE

text: urban biome

text: human-associated habitat

text: human-oral

text: organic material

text: USA:CA

text: human

characteristic: host common name

text: Homo sapiens

characteristic: host scientific name

characteristic: host subject id

characteristic: host taxid

text: n

characteristic: improve

characteristic: individual

characteristic: inflammation

characteristic: latitude

characteristic: longitude

text: human oral metagenome

text: A

characteristic: pdepthreclass

text: SDSU

text: FALSE

text: A

characteristic: pocketclass

characteristic: pocketdepth

text: dental plaque

characteristic: sample type

text: bx

characteristic: samplesite

text: human oral metagenome

text: mesio-lingual

characteristic: time

characteristic: timepoint

text: lower left central incisor

text: T