E-MTAB-5349:Sample 36

characteristic: ENA-FIRST-PUBLIC

text: ETH Zurich, Switzerland

text: public

text: E-MTAB-5349:Sample 36

tag: Namespace:ETH Zurich, Switzerland

text: 8 to 10

unit: week

text: ArrayExpress

characteristic: broker name

text: long lived plasma cell

characteristic: cell type

text: house mouse

text: Protocols: Single-cell suspensions were prepared from harvested bone marrow and spleen as previously described (Menzel et al., PLOS ONE, 2014, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4014543/). Red blood cells were lysed (RBC lysis buffer, eBioscience) and Fc receptor block was performed using rat serum to inhibit non-specific binding of rat-derived labeling antibodies. Bone marrow cells were labeled with a panel of fluorescence-conjugated antibodies consisting of CD19-FITC (eBioscience, clone 1D3), CD138-PE (BD, clone 281-2), CD22-APC (Biolegend, clone OX-97), MHCII-APC-Cy7 (Biolegend, clone M5/114.15.2), IgM-BV510 (BD, clone R6-60.2), CD25-PE-Cy7 (Biolegend, clone PC61), c-kit-BV421 (Biolegend, clone 2B8). Splenocytes were stained with an antibody panel consisting of CD19-FITC, CD138-PE, CD22-APC, MHCII-APC-Cy7, IgM-BV510, IgD-PerCP-Cy5.5 (Biolegend, clone 11-26c.2a), CD21-BV421 (Biolegend, clone 7E9) and CD23-PE-Cy7 (Biolegend, clone B3B4). Cells were sorted on a BD FACS Aria II and analyzed using FACS Diva software. Compensation was performed with single stain controls (UltraComp eBeads, eBioscience) and fluorescence minus one (FMO) controls were used to set the gates. The following cellular populations were isolated: pre-B cells from bone marrow (c-kit-CD19+IgM-CD25+) , long-lived memory plasma cells from bone marrow (CD138+CD22-MHCII-CD19-IgM-) and naive follicular B cells from spleen (CD138-CD19+IgD++IgM+CD23++CD21+). All cells were sorted directly into TRIzol (Life Technologies) and stored at -80°C. Naive B cells of pet mice were sorted into TRIzol from CD19-MACS-enriched splenocytes based on CD19+CD38+IgM+IgD+IgG2b-CD93-CD138-F4/80-CD11c-GL7-PI-. All mouse experiments were performed under the guidelines and protocols approved by the Basel-Stadt cantonal veterinary office (Basel-Stadt Kantonales Veterinäramt Tierversuchsbewilligung #2582). Four cohorts of five female C57BL/6 J mice (Janvier Laboratories, France) 8-10 weeks old were housed under specific pathogen-free conditions and food and water were provided ad libitum. A prime-boost immunization scheme was applied to three cohorts of mice. Primary immunizations of three cohorts consisted of intraperitoneal injections of alum-precipitated antigens. Antigen dosage per mouse was: 100 µg ovalbumin (OVA, Invivogen), 100 µg 4-hydroxy-3-nitrophenylacetyl-conjugated hen egg lysozyme (NP-HEL, Biosearch Technologies), 4 µg Hepatitis B virus surface antigen (HBsAg, Cell Sciences). Mice were boosted intraperitoneally after three weeks with identical amounts of antigen as used in primary in PBS. Immunized mice were sacrificed 14 days post-secondary immunization; untreated control mice were sacrificed at corresponding age. Spleen, bone marrow (from femur, tibia, pelvis and sternum) and blood were collected from all mice Four untreated female Balb/c mice (Janvier Laboratories, France) were housed under the same conditions and spleens were isolated at 11-weeks of age. Three pet mice of unknown genetic background (white fur) were obtained as adult mice at pet shops in Berlin. All pet mice experiments were performed according to institutional guidelines and licensed under German animal protection regulations. Antibody variable heavy chain (VH) libraries compatible for sequencing with Illumina MiSeq (paired-end 2x300bp) platform were prepared similarly to a previously described two-step primer extension method (Menzel et al., 2014, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4014543/). We ensured full biological coverage by using total RNA material for RT-PCR and avoided subsampling effects by using entire RT-PCR products for each following step. Total RNA was extracted using the TRIzol Plus RNA Purification Kit (Life Technologies) according to the manufacturer’s protocol. RNA integrity and concentration were evaluated on a Bioanalyzer 2100 (Agilent) for at least two mice per cohort. Average RNA integrity values of all tested samples were >8.5. First-strand cDNA was synthesized from total RNA using AccuScript Hi-Fi reverse transcriptase (Agilent Technologies) and Oligo(dT)18 primers (Thermo Scientific) following the manufacturer’s protocol. Parallel reverse transcription reactions were pooled followed by a 0.8X left-sided SPRIselect bead cleanup (Beckman Coulter). The 20 µL of cDNA bead elution was subjected to a first PCR step. Amplification was performed using a forward mix of primers (specific to framework region 1) containing an extension 1 region. PreBC and nBC were amplified with an extension 2-containing IgM-specific reverse primer, while PC were amplified with IgG- and IgM-specific reverse primers. PCR was performed with Q5 High-Fidelity DNA polymerase (NEB) in a reaction volume of 50 µl with the following cycle conditions: 98°C for 30 sec; 4 cycles of 98°C for 10 sec, 50°C for 20 sec, 72°C for 1 min; 4 cycles of 98°C for 10 sec, 55°C for 20 sec, 72°C for 1 min; 8-12 cycles (depending on cell population) of 98°C for 10 sec, 63°C for 20 sec, 72°C for 1 min; 72°C for 5 min; 4°C storage. PCR products were concentrated using DNA Clean & Concentrator (Zymo Research) followed by 0.8X SPRIselect left-sided size selection. Total PCR1 product was amplified in a PCR2 step, which added extension-specific full-length Illumina adapter sequences to the amplicon library. Individual samples were Illumina-indexed by choosing from 20 different index reverse primers. Cycle conditions were as follows: 98°C for 30 sec; 2 cycles of 98°C for 10 sec, 40°C for 20 sec, 72°C for 1 min; 5-9 cycles (depending on cell population) of 98°C for 10 sec, 65°C for 20 sec, 72°C for 1 min; 72°C for 5 min; 4°C storage. PCR2 products were concentrated again (Zymo Research) and run on a 1% agarose gel. Bands of appropriate size (~560bp) were gel-purified using the MinElute Gel Extraction Kit (Qiagen). Purified libraries were quantified by the KAPA library quantification kit for Illumina platforms (KAPA Biosystems) and pooled at concentrations aimed at optimal read return. The quality of the final sequencing pool was verified on a fragment analyzer (Advanced Analytical Technologies) using DNF-473 Standard Sensitivity NGS fragment analysis kit. All samples passed quality control and were sequenced. Replicate sequence was performed as previously described (Menzel et al., 2014): technical replicates (two per sample) of Ig-seq libraries were prepared from preBC, nBC and PC RNA derived from a single OVA-immunized mouse. RNA was split and library preparation was performed in parallel with different Illumina indices as described above.

characteristic: description

text: HP-HEL mouse 1

characteristic: individual

text: Mus musculus

text: Mus musculus

text: bone marrow

characteristic: organism part

text: Mus musculus

text: female

text: Sample 36


E-MTAB-5349:Sample 34

status: PUBLIC

tag: attribute

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ETH Zurich, Switzerland

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-5349:Sample 34

characteristic: Submitter Id

text: 8 to 10

characteristic: age

text: ArrayExpress

text: pre-B cell

text: house mouse

characteristic: common name

text: HP-HEL mouse 1

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

text: Mus musculus

text: female

characteristic: sex

characteristic: strain

text: Sample 34

characteristic: title

type: has member


E-MTAB-5349:Sample 35

characteristic: ENA-FIRST-PUBLIC

text: ETH Zurich, Switzerland

text: public

text: E-MTAB-5349:Sample 35

text: 8 to 10

text: ArrayExpress

characteristic: broker name

text: naive B cell

characteristic: cell type

text: house mouse

characteristic: description

text: HP-HEL mouse 1

characteristic: individual

text: Mus musculus

text: Mus musculus

text: spleen

text: Mus musculus

text: female

text: Sample 35


E-MTAB-5349:Sample 70

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ETH Zurich, Switzerland

characteristic: INSDC center name

text: public

text: E-MTAB-5349:Sample 70

text: 8 to 10

text: ArrayExpress

text: naive B cell

text: house mouse

text: Protocols: Single-cell suspensions were prepared from harvested bone marrow and spleen as previously described (Menzel et al., PLOS ONE, 2014, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4014543/). Red blood cells were lysed (RBC lysis buffer, eBioscience) and Fc receptor block was performed using rat serum to inhibit non-specific binding of rat-derived labeling antibodies. Bone marrow cells were labeled with a panel of fluorescence-conjugated antibodies consisting of CD19-FITC (eBioscience, clone 1D3), CD138-PE (BD, clone 281-2), CD22-APC (Biolegend, clone OX-97), MHCII-APC-Cy7 (Biolegend, clone M5/114.15.2), IgM-BV510 (BD, clone R6-60.2), CD25-PE-Cy7 (Biolegend, clone PC61), c-kit-BV421 (Biolegend, clone 2B8). Splenocytes were stained with an antibody panel consisting of CD19-FITC, CD138-PE, CD22-APC, MHCII-APC-Cy7, IgM-BV510, IgD-PerCP-Cy5.5 (Biolegend, clone 11-26c.2a), CD21-BV421 (Biolegend, clone 7E9) and CD23-PE-Cy7 (Biolegend, clone B3B4). Cells were sorted on a BD FACS Aria II and analyzed using FACS Diva software. Compensation was performed with single stain controls (UltraComp eBeads, eBioscience) and fluorescence minus one (FMO) controls were used to set the gates. The following cellular populations were isolated: pre-B cells from bone marrow (c-kit-CD19+IgM-CD25+) , long-lived memory plasma cells from bone marrow (CD138+CD22-MHCII-CD19-IgM-) and naive follicular B cells from spleen (CD138-CD19+IgD++IgM+CD23++CD21+). All cells were sorted directly into TRIzol (Life Technologies) and stored at -80°C. Naive B cells of pet mice were sorted into TRIzol from CD19-MACS-enriched splenocytes based on CD19+CD38+IgM+IgD+IgG2b-CD93-CD138-F4/80-CD11c-GL7-PI-. All mouse experiments were performed under the guidelines and protocols approved by the Basel-Stadt cantonal veterinary office (Basel-Stadt Kantonales Veterinäramt Tierversuchsbewilligung #2582). Four cohorts of five female C57BL/6 J mice (Janvier Laboratories, France) 8-10 weeks old were housed under specific pathogen-free conditions and food and water were provided ad libitum. A prime-boost immunization scheme was applied to three cohorts of mice. Primary immunizations of three cohorts consisted of intraperitoneal injections of alum-precipitated antigens. Antigen dosage per mouse was: 100 µg ovalbumin (OVA, Invivogen), 100 µg 4-hydroxy-3-nitrophenylacetyl-conjugated hen egg lysozyme (NP-HEL, Biosearch Technologies), 4 µg Hepatitis B virus surface antigen (HBsAg, Cell Sciences). Mice were boosted intraperitoneally after three weeks with identical amounts of antigen as used in primary in PBS. Immunized mice were sacrificed 14 days post-secondary immunization; untreated control mice were sacrificed at corresponding age. Spleen, bone marrow (from femur, tibia, pelvis and sternum) and blood were collected from all mice Four untreated female Balb/c mice (Janvier Laboratories, France) were housed under the same conditions and spleens were isolated at 11-weeks of age. Three pet mice of unknown genetic background (white fur) were obtained as adult mice at pet shops in Berlin. All pet mice experiments were performed according to institutional guidelines and licensed under German animal protection regulations. Two naive B cell samples (10^6, cells) from an NP-HEL and HBsAg immunized mouse were used for Ig-seq library preparation based on a method of unique molecular identifier (UID)-tagging, as previously described (Khan et al., 2016,https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/26998518/). UID-tagging was adapted to IgM-isotype specificity. The nBC RNA samples were split (replicates) and first-strand cDNA was generated using an IgM constant region-specific primer containing a reverse degenerate nucleotide UID and a partial Illumina adapter extension region. First-strand cDNA was purified by 0.8X left-sided SPRIselect bead cleanup and synthetic spike-in genes (10%) were added (Khan et al., 2016). PCR1 was performed using Q5 High-Fidelity DNA polymerase (NEB), with a forward primer set (specific for framework 1 region, same primerset as used in the primer extension method) including forward UIDs and a partial Illumina adapter sequence region and a partial Illumina adapter reverse primer. PCR1 cycle conditions were: 98°C for 30 sec; 4 cycles of 98°C for 10 sec, 50°C for 20 sec, 72°C for 1 min; 4 cycles of 98°C for 10 sec, 55°C for 20 sec, 72°C for 1 min; 5 cycles of 98°C for 10 sec, 63°C for 20 sec, 72°C for 1 min; 72°C for 5 min; 4°C storage. This was followed by a left-sided size selection using 0.8X SPRIselect beads. The PCR2 step attached full-length Illumina Universal Adapter and Index Adapter sequences to the library and was performed with 98°C for 30 sec; 19 cycles of 98°C for 10 sec, 65°C for 20 sec, 72°C for 1 min; 4°C storage. Final amplicon products were purified by a two-sided 0.5-0.8X SPRIselect bead cleanup step and were submitted for fragment analysis quality control. UID-tagged samples were pooled at equimolar concentration.

text: HP-HEL mouse 1

text: Mus musculus

text: Mus musculus

text: spleen

characteristic: organism part

text: Mus musculus

text: female

text: Sample 70


E-MTAB-5349:Sample 71

text: ETH Zurich, Switzerland

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-5349:Sample 71

characteristic: Submitter Id

text: 8 to 10

text: ArrayExpress

text: naive B cell

text: house mouse

text: HP-HEL mouse 1

text: Mus musculus

text: Mus musculus

text: spleen

text: Mus musculus

text: female

text: Sample 71


E-MTAB-5349:Sample 39

text: ETH Zurich, Switzerland

text: public

text: E-MTAB-5349:Sample 39

text: 8 to 10

characteristic: age

text: ArrayExpress

text: long lived plasma cell

text: house mouse

characteristic: common name

text: HP-HEL mouse 2

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

text: Mus musculus

text: female

characteristic: sex

characteristic: strain

text: Sample 39


E-MTAB-5349:Sample 37

characteristic: ENA-FIRST-PUBLIC

text: ETH Zurich, Switzerland

text: public

text: E-MTAB-5349:Sample 37

text: 8 to 10

text: ArrayExpress

characteristic: broker name

text: pre-B cell

characteristic: cell type

text: house mouse

characteristic: description

text: HP-HEL mouse 2

text: Mus musculus

text: Mus musculus

text: bone marrow

text: Mus musculus

text: female

text: Sample 37

characteristic: title


E-MTAB-5349:Sample 38

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ETH Zurich, Switzerland

characteristic: INSDC center name

text: public

text: E-MTAB-5349:Sample 38

text: 8 to 10

text: ArrayExpress

text: naive B cell

text: house mouse

text: HP-HEL mouse 2

characteristic: individual

text: Mus musculus

text: Mus musculus

text: spleen

text: Mus musculus

text: female

text: Sample 38


E-MTAB-5349:Sample 42

text: ETH Zurich, Switzerland

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-5349:Sample 42

text: 8 to 10

text: ArrayExpress

text: long lived plasma cell

text: house mouse

text: HP-HEL mouse 3

text: Mus musculus

text: Mus musculus

text: bone marrow

characteristic: organism part

text: Mus musculus

text: female

text: Sample 42


E-MTAB-5349:Sample 40

text: ETH Zurich, Switzerland

text: public

text: E-MTAB-5349:Sample 40

characteristic: Submitter Id

text: 8 to 10

characteristic: age

text: ArrayExpress

text: pre-B cell

text: house mouse

characteristic: common name

text: HP-HEL mouse 3

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

text: Mus musculus

text: female

characteristic: sex

text: Sample 40


E-MTAB-5349:Sample 41

characteristic: ENA-FIRST-PUBLIC

text: ETH Zurich, Switzerland

text: public

text: E-MTAB-5349:Sample 41

text: 8 to 10

text: ArrayExpress

characteristic: broker name

text: naive B cell

characteristic: cell type

text: house mouse

characteristic: description

text: HP-HEL mouse 3

text: Mus musculus

text: Mus musculus

text: spleen

text: Mus musculus

text: female

characteristic: strain

text: Sample 41


E-MTAB-5349:Sample 45

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ETH Zurich, Switzerland

characteristic: INSDC center name

text: public

text: E-MTAB-5349:Sample 45

text: 8 to 10

text: ArrayExpress

text: long lived plasma cell

text: house mouse

text: HP-HEL mouse 4

text: Mus musculus

text: Mus musculus

text: bone marrow

text: Mus musculus

text: female

text: Sample 45

characteristic: title


E-MTAB-5349:Sample 43

text: ETH Zurich, Switzerland

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-5349:Sample 43

text: 8 to 10

text: ArrayExpress

text: pre-B cell

text: house mouse

text: HP-HEL mouse 4

characteristic: individual

text: Mus musculus

text: Mus musculus

text: bone marrow

text: Mus musculus

text: female

text: Sample 43


E-MTAB-5349:Sample 44

text: ETH Zurich, Switzerland

text: public

text: E-MTAB-5349:Sample 44

characteristic: Submitter Id

text: 8 to 10

characteristic: age

text: ArrayExpress

text: naive B cell

text: house mouse

characteristic: common name

text: HP-HEL mouse 4

text: Mus musculus

characteristic: organism

text: Mus musculus

text: spleen

characteristic: organism part

text: Mus musculus

text: female

text: Sample 44


E-MTAB-5349:Sample 48

characteristic: ENA-FIRST-PUBLIC

text: ETH Zurich, Switzerland

text: public

text: E-MTAB-5349:Sample 48

text: 8 to 10

text: ArrayExpress

characteristic: broker name

text: long lived plasma cell

characteristic: cell type

text: house mouse

characteristic: description

text: HP-HEL mouse 5

text: Mus musculus

text: Mus musculus

text: bone marrow

text: Mus musculus

text: female

characteristic: sex

text: Sample 48


E-MTAB-5349:Sample 46

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ETH Zurich, Switzerland

characteristic: INSDC center name

text: public

text: E-MTAB-5349:Sample 46

text: 8 to 10

text: ArrayExpress

text: pre-B cell

text: house mouse

text: HP-HEL mouse 5

text: Mus musculus

text: Mus musculus

text: bone marrow

text: Mus musculus

text: female

characteristic: strain

text: Sample 46


E-MTAB-5349:Sample 47

text: ETH Zurich, Switzerland

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-5349:Sample 47

text: 8 to 10

text: ArrayExpress

text: naive B cell

text: house mouse

text: HP-HEL mouse 5

text: Mus musculus

text: Mus musculus

text: spleen

text: Mus musculus

text: female

text: Sample 47

characteristic: title


E-MTAB-5349:Sample 51

text: ETH Zurich, Switzerland

text: public

text: E-MTAB-5349:Sample 51

characteristic: Submitter Id

text: 8 to 10

characteristic: age

text: ArrayExpress

text: long lived plasma cell

text: house mouse

characteristic: common name

text: HBsAg mouse 1

characteristic: individual

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

text: Mus musculus

text: female

text: Sample 51


E-MTAB-5349:Sample 49

characteristic: ENA-FIRST-PUBLIC

text: ETH Zurich, Switzerland

text: public

text: E-MTAB-5349:Sample 49

text: 8 to 10

text: ArrayExpress

characteristic: broker name

text: pre-B cell

characteristic: cell type

text: house mouse

characteristic: description

text: HBsAg mouse 1

text: Mus musculus

text: Mus musculus

text: bone marrow

characteristic: organism part

text: Mus musculus

text: female

text: Sample 49


E-MTAB-5349:Sample 50

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ETH Zurich, Switzerland

characteristic: INSDC center name

text: public

text: E-MTAB-5349:Sample 50

text: 8 to 10

text: ArrayExpress

text: naive B cell

text: house mouse

text: HBsAg mouse 1

text: Mus musculus

text: Mus musculus

text: spleen

text: Mus musculus

text: female

characteristic: sex

text: Sample 50


E-MTAB-5349:Sample 54

text: ETH Zurich, Switzerland

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-5349:Sample 54

text: 8 to 10

text: ArrayExpress

text: long lived plasma cell

text: house mouse

text: HBsAg mouse 2

text: Mus musculus

text: Mus musculus

text: bone marrow

text: Mus musculus

text: female

characteristic: strain

text: Sample 54


E-MTAB-5349:Sample 52

text: ETH Zurich, Switzerland

text: public

text: E-MTAB-5349:Sample 52

characteristic: Submitter Id

text: 8 to 10

characteristic: age

text: ArrayExpress

text: pre-B cell

text: house mouse

characteristic: common name

text: HBsAg mouse 2

characteristic: individual

text: Mus musculus

text: Mus musculus

text: bone marrow

text: Mus musculus

text: female

text: Sample 52

characteristic: title


E-MTAB-5349:Sample 53

characteristic: ENA-FIRST-PUBLIC

text: ETH Zurich, Switzerland

text: public

text: E-MTAB-5349:Sample 53

text: 8 to 10

text: ArrayExpress

characteristic: broker name

text: naive B cell

characteristic: cell type

text: house mouse

characteristic: description

text: HBsAg mouse 2

text: Mus musculus

characteristic: organism

text: Mus musculus

text: spleen

text: Mus musculus

text: female

text: Sample 53


E-MTAB-5349:Sample 57

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ETH Zurich, Switzerland

characteristic: INSDC center name

text: public

text: E-MTAB-5349:Sample 57

text: 8 to 10

text: ArrayExpress

text: long lived plasma cell

text: house mouse

text: HBsAg mouse 3

text: Mus musculus

text: Mus musculus

text: bone marrow

characteristic: organism part

text: Mus musculus

text: female

text: Sample 57


E-MTAB-5349:Sample 55

text: ETH Zurich, Switzerland

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-5349:Sample 55

text: 8 to 10

text: ArrayExpress

text: pre-B cell

text: house mouse

text: HBsAg mouse 3

text: Mus musculus

text: Mus musculus

text: bone marrow

text: Mus musculus

text: female

characteristic: sex

text: Sample 55


E-MTAB-5349:Sample 56

text: ETH Zurich, Switzerland

text: public

text: E-MTAB-5349:Sample 56

characteristic: Submitter Id

text: 8 to 10

characteristic: age

text: ArrayExpress

text: naive B cell

text: house mouse

characteristic: common name

text: HBsAg mouse 3

characteristic: individual

text: Mus musculus

text: Mus musculus

text: spleen

text: Mus musculus

text: female

characteristic: strain

text: Sample 56


E-MTAB-5349:Sample 60

characteristic: ENA-FIRST-PUBLIC

text: ETH Zurich, Switzerland

text: public

text: E-MTAB-5349:Sample 60

text: 8 to 10

text: ArrayExpress

characteristic: broker name

text: long lived plasma cell

characteristic: cell type

text: house mouse

characteristic: description

text: HBsAg mouse 4

text: Mus musculus

text: Mus musculus

text: bone marrow

text: Mus musculus

text: female

text: Sample 60

characteristic: title


E-MTAB-5349:Sample 58

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ETH Zurich, Switzerland

characteristic: INSDC center name

text: public

text: E-MTAB-5349:Sample 58

text: 8 to 10

text: ArrayExpress

text: pre-B cell

text: house mouse

text: HBsAg mouse 4

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

text: Mus musculus

text: female

text: Sample 58


E-MTAB-5349:Sample 59

text: ETH Zurich, Switzerland

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-5349:Sample 59

text: 8 to 10

text: ArrayExpress

text: naive B cell

text: house mouse

text: HBsAg mouse 4

text: Mus musculus

text: Mus musculus

text: spleen

characteristic: organism part

text: Mus musculus

text: female

text: Sample 59


E-MTAB-5349:Sample 68

text: ETH Zurich, Switzerland

text: public

text: E-MTAB-5349:Sample 68

characteristic: Submitter Id

text: 8 to 10

characteristic: age

text: ArrayExpress

text: naive B cell

text: house mouse

characteristic: common name

text: HBsAg mouse 5

characteristic: individual

text: Mus musculus

text: Mus musculus

text: spleen

text: Mus musculus

text: female

characteristic: sex

text: Sample 68


E-MTAB-5349:Sample 69

characteristic: ENA-FIRST-PUBLIC

text: ETH Zurich, Switzerland

text: public

text: E-MTAB-5349:Sample 69

text: 8 to 10

text: ArrayExpress

characteristic: broker name

text: naive B cell

characteristic: cell type

text: house mouse

characteristic: description

text: HBsAg mouse 5

text: Mus musculus

text: Mus musculus

text: spleen

text: Mus musculus

text: female

characteristic: strain

text: Sample 69


E-MTAB-5349:Sample 61

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ETH Zurich, Switzerland

characteristic: INSDC center name

text: public

text: E-MTAB-5349:Sample 61

text: ArrayExpress

text: naive B cell

text: house mouse

text: Balb/c mouse 1

text: Mus musculus

text: Mus musculus

text: spleen

text: Mus musculus

text: female

text: Balb/c

text: Sample 61

characteristic: title


E-MTAB-5349:Sample 62

text: ETH Zurich, Switzerland

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-5349:Sample 62

text: ArrayExpress

text: naive B cell

text: house mouse

text: Balb/c mouse 2

text: Mus musculus

characteristic: organism

text: Mus musculus

text: spleen

text: Mus musculus

text: female

text: Balb/c

text: Sample 62


E-MTAB-5349:Sample 63

text: ETH Zurich, Switzerland

text: public

text: E-MTAB-5349:Sample 63

characteristic: Submitter Id

characteristic: age

text: ArrayExpress

text: naive B cell

text: house mouse

characteristic: common name

text: Balb/c mouse 3

characteristic: individual

text: Mus musculus

text: Mus musculus

text: spleen

characteristic: organism part

text: Mus musculus

text: female

text: Balb/c

text: Sample 63


E-MTAB-5349:Sample 64

characteristic: ENA-FIRST-PUBLIC

text: ETH Zurich, Switzerland

text: public

text: E-MTAB-5349:Sample 64

text: ArrayExpress

characteristic: broker name

text: naive B cell

characteristic: cell type

text: house mouse

characteristic: description

text: Balb/c mouse 4

text: Mus musculus

text: Mus musculus

text: spleen

text: Mus musculus

text: female

characteristic: sex

text: Balb/c

text: Sample 64


E-MTAB-5349:Sample 3

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ETH Zurich, Switzerland

characteristic: INSDC center name

text: public

text: E-MTAB-5349:Sample 3

text: 8 to 10

text: ArrayExpress

text: long lived plasma cell

text: house mouse

text: C57BL/6 mouse 1

text: Mus musculus

text: Mus musculus

text: bone marrow

text: Mus musculus

text: female

characteristic: strain

text: Sample 3


E-MTAB-5349:Sample 1

text: ETH Zurich, Switzerland

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-5349:Sample 1

text: 8 to 10

text: ArrayExpress

text: pre-B cell

text: house mouse

text: C57BL/6 mouse 1

text: Mus musculus

text: Mus musculus

text: bone marrow

text: Mus musculus

text: female

text: Sample 1

characteristic: title


text: Plasmodium sp. gorilla clade G1

characteristic: ArrayExpress-Species

text: SC

text: public

characteristic: Submitter Id

text: Plasmodium sp. gorilla clade G1

characteristic: common name

text: Plasmodium sp. gorilla clade G1

characteristic: organism

text: PacBio

characteristic: sample description

text: Plasmodium sp. gorilla clade G1

text: Plasmodium praefalciparum G01


text: Plasmodium reichenowi

characteristic: ENA-FIRST-PUBLIC

text: SC

text: public

text: Plasmodium reichenowi

text: Plasmodium reichenowi

text: PacBio

text: Plasmodium reichenowi

text: Plasmodium reichenowi G01


E-MTAB-5349:Sample 2

text: ETH Zurich, Switzerland

text: public

text: E-MTAB-5349:Sample 2

text: 8 to 10

characteristic: age

text: ArrayExpress

characteristic: broker name

text: naive B cell

characteristic: cell type

text: house mouse

characteristic: description

text: C57BL/6 mouse 1

characteristic: individual

text: Mus musculus

text: Mus musculus

text: spleen

characteristic: organism part

text: Mus musculus

text: female

characteristic: sex

text: Sample 2


E-MTAB-5349:Sample 6

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: ETH Zurich, Switzerland

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-5349:Sample 6

text: 8 to 10

text: ArrayExpress

text: long lived plasma cell

text: house mouse

text: C57BL/6 mouse 2

text: Mus musculus

text: Mus musculus

text: bone marrow

text: Mus musculus

text: female

characteristic: strain

text: Sample 6


E-MTAB-5349:Sample 4

text: ETH Zurich, Switzerland

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-5349:Sample 4

characteristic: Submitter Id

text: 8 to 10

text: ArrayExpress

text: pre-B cell

text: house mouse

characteristic: common name

text: C57BL/6 mouse 2

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

text: Mus musculus

text: female

text: Sample 4

characteristic: title


E-MTAB-5349:Sample 5

characteristic: ENA-FIRST-PUBLIC

text: ETH Zurich, Switzerland

text: public

text: E-MTAB-5349:Sample 5

text: 8 to 10

characteristic: age

text: ArrayExpress

characteristic: broker name

text: naive B cell

characteristic: cell type

text: house mouse

characteristic: description

text: C57BL/6 mouse 2

text: Mus musculus

text: Mus musculus

text: spleen

text: Mus musculus

text: female

text: Sample 5


E-MTAB-5349:Sample 9

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: ETH Zurich, Switzerland

text: public

text: E-MTAB-5349:Sample 9

text: 8 to 10

text: ArrayExpress

text: long lived plasma cell

text: house mouse

text: C57BL/6 mouse 3

characteristic: individual

text: Mus musculus

text: Mus musculus

text: bone marrow

characteristic: organism part

text: Mus musculus

text: female

characteristic: sex

text: Sample 9


E-MTAB-5349:Sample 7

characteristic: External Id

text: ETH Zurich, Switzerland

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-5349:Sample 7

text: 8 to 10

text: ArrayExpress

text: pre-B cell

text: house mouse

text: C57BL/6 mouse 3

text: Mus musculus

text: Mus musculus

text: bone marrow

text: Mus musculus

text: female

characteristic: strain

text: Sample 7


E-MTAB-5349:Sample 8

text: ETH Zurich, Switzerland

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-5349:Sample 8

characteristic: Submitter Id

text: 8 to 10

text: ArrayExpress

text: naive B cell

text: house mouse

characteristic: common name

text: C57BL/6 mouse 3

text: Mus musculus

characteristic: organism

text: Mus musculus

text: spleen

text: Mus musculus

text: female

text: Sample 8


E-MTAB-5349:Sample 12

characteristic: ENA-FIRST-PUBLIC

text: ETH Zurich, Switzerland

text: public

text: E-MTAB-5349:Sample 12

text: 8 to 10

characteristic: age

text: ArrayExpress

characteristic: broker name

text: long lived plasma cell

characteristic: cell type

text: house mouse

text: C57BL/6 mouse 4

text: Mus musculus

text: Mus musculus

text: bone marrow

text: Mus musculus

text: female

text: Sample 12

characteristic: title


E-MTAB-5349:Sample 10

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: ETH Zurich, Switzerland

text: public

text: E-MTAB-5349:Sample 10

text: 8 to 10

text: ArrayExpress

text: pre-B cell

text: house mouse

characteristic: description

text: C57BL/6 mouse 4

characteristic: individual

text: Mus musculus

text: Mus musculus

text: bone marrow

characteristic: organism part

text: Mus musculus

text: female

text: Sample 10


E-MTAB-5349:Sample 11

characteristic: External Id

text: ETH Zurich, Switzerland

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-5349:Sample 11

text: 8 to 10

text: ArrayExpress

text: naive B cell

text: house mouse

text: C57BL/6 mouse 4

text: Mus musculus

text: Mus musculus

text: spleen

text: Mus musculus

text: female

characteristic: sex

text: Sample 11


E-MTAB-5349:Sample 15

text: ETH Zurich, Switzerland

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-5349:Sample 15

characteristic: Submitter Id

text: 8 to 10

text: ArrayExpress

text: long lived plasma cell

text: house mouse

characteristic: common name

text: C57BL/6 mouse 5

text: Mus musculus

text: Mus musculus

text: bone marrow

text: Mus musculus

text: female

characteristic: strain

text: Sample 15


E-MTAB-5349:Sample 13

characteristic: ENA-FIRST-PUBLIC

text: ETH Zurich, Switzerland

text: public

text: E-MTAB-5349:Sample 13

text: 8 to 10

characteristic: age

text: ArrayExpress

characteristic: broker name

text: pre-B cell

characteristic: cell type

text: house mouse

text: C57BL/6 mouse 5

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

text: Mus musculus

text: female

text: Sample 13


E-MTAB-5349:Sample 14

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: ETH Zurich, Switzerland

text: public

text: E-MTAB-5349:Sample 14

text: 8 to 10

text: ArrayExpress

text: naive B cell

text: house mouse

characteristic: description

text: C57BL/6 mouse 5

characteristic: individual

text: Mus musculus

text: Mus musculus

text: spleen

text: Mus musculus

text: female

text: Sample 14

characteristic: title


E-MTAB-5349:Sample 65

characteristic: External Id

text: ETH Zurich, Switzerland

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-5349:Sample 65

text: adult

text: ArrayExpress

text: naive B cell

text: house mouse

text: pet shop mouse 1

text: Mus musculus

text: Mus musculus

text: spleen

characteristic: organism part

text: Mus musculus

text: female

text: pet shop mouse

text: Sample 65


E-MTAB-5349:Sample 66

text: ETH Zurich, Switzerland

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-5349:Sample 66

characteristic: Submitter Id

text: adult

text: ArrayExpress

text: naive B cell

text: house mouse

characteristic: common name

text: pet shop mouse 2

text: Mus musculus

text: Mus musculus

text: spleen

text: Mus musculus

text: female

characteristic: sex

text: pet shop mouse

text: Sample 66


E-MTAB-5349:Sample 67

characteristic: ENA-FIRST-PUBLIC

text: ETH Zurich, Switzerland

text: public

text: E-MTAB-5349:Sample 67

text: adult

characteristic: age

text: ArrayExpress

characteristic: broker name

text: naive B cell

characteristic: cell type

text: house mouse

text: pet shop mouse 3

text: Mus musculus

text: Mus musculus

text: spleen

text: Mus musculus

text: female

text: pet shop mouse

characteristic: strain

text: Sample 67


E-MTAB-5349:Sample 18

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: ETH Zurich, Switzerland

text: public

text: E-MTAB-5349:Sample 18

text: 8 to 10

text: ArrayExpress

text: long lived plasma cell

text: house mouse

characteristic: description

text: OVA mouse 1

characteristic: individual

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

text: Mus musculus

text: female

text: Sample 18


E-MTAB-5349:Sample 16

characteristic: External Id

text: ETH Zurich, Switzerland

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-5349:Sample 16

text: 8 to 10

text: ArrayExpress

text: pre-B cell

text: house mouse

text: OVA mouse 1

text: Mus musculus

text: Mus musculus

text: bone marrow

text: Mus musculus

text: female

text: Sample 16

characteristic: title


E-MTAB-5349:Sample 17

text: ETH Zurich, Switzerland

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-5349:Sample 17

characteristic: Submitter Id

text: 8 to 10

text: ArrayExpress

text: naive B cell

text: house mouse

characteristic: common name

text: OVA mouse 1

text: Mus musculus

text: Mus musculus

text: spleen

characteristic: organism part

text: Mus musculus

text: female

text: Sample 17


E-MTAB-5349:Sample 21

characteristic: ENA-FIRST-PUBLIC

text: ETH Zurich, Switzerland

text: public

text: E-MTAB-5349:Sample 21

text: 8 to 10

characteristic: age

text: ArrayExpress

characteristic: broker name

text: long lived plasma cell

characteristic: cell type

text: house mouse

text: OVA mouse 2

text: Mus musculus

text: Mus musculus

text: bone marrow

text: Mus musculus

text: female

characteristic: sex

text: Sample 21


E-MTAB-5349:Sample 19

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: ETH Zurich, Switzerland

text: public

text: E-MTAB-5349:Sample 19

text: 8 to 10

text: ArrayExpress

text: pre-B cell

text: house mouse

characteristic: description

text: OVA mouse 2

characteristic: individual

text: Mus musculus

text: Mus musculus

text: bone marrow

text: Mus musculus

text: female

characteristic: strain

text: Sample 19


E-MTAB-5349:Sample 20

characteristic: External Id

text: ETH Zurich, Switzerland

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-5349:Sample 20

text: 8 to 10

text: ArrayExpress

text: naive B cell

text: house mouse

text: OVA mouse 2

text: Mus musculus

characteristic: organism

text: Mus musculus

text: spleen

text: Mus musculus

text: female

text: Sample 20


E-MTAB-5349:Sample 24

text: ETH Zurich, Switzerland

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-5349:Sample 24

characteristic: Submitter Id

text: 8 to 10

text: ArrayExpress

text: long lived plasma cell

text: house mouse

characteristic: common name

text: OVA mouse 3

text: Mus musculus

text: Mus musculus

text: bone marrow

text: Mus musculus

text: female

text: Sample 24

characteristic: title


E-MTAB-5349:Sample 22

characteristic: ENA-FIRST-PUBLIC

text: ETH Zurich, Switzerland

text: public

text: E-MTAB-5349:Sample 22

text: 8 to 10

characteristic: age

text: ArrayExpress

characteristic: broker name

text: pre-B cell

characteristic: cell type

text: house mouse

text: OVA mouse 3

text: Mus musculus

text: Mus musculus

text: bone marrow

characteristic: organism part

text: Mus musculus

text: female

text: Sample 22


E-MTAB-5349:Sample 23

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: ETH Zurich, Switzerland

text: public

text: E-MTAB-5349:Sample 23

text: 8 to 10

text: ArrayExpress

text: naive B cell

text: house mouse

characteristic: description

text: OVA mouse 3

characteristic: individual

text: Mus musculus

text: Mus musculus

text: spleen

text: Mus musculus

text: female

characteristic: sex

text: Sample 23


E-MTAB-5349:Sample 27

characteristic: External Id

text: ETH Zurich, Switzerland

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-5349:Sample 27

text: 8 to 10

text: ArrayExpress

text: long lived plasma cell

text: house mouse

text: OVA mouse 4

text: Mus musculus

text: Mus musculus

text: bone marrow

text: Mus musculus

text: female

characteristic: strain

text: Sample 27


E-MTAB-5349:Sample 25

text: ETH Zurich, Switzerland

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-5349:Sample 25

characteristic: Submitter Id

text: 8 to 10

text: ArrayExpress

text: pre-B cell

text: house mouse

characteristic: common name

text: OVA mouse 4

text: Mus musculus

characteristic: organism

text: Mus musculus

text: bone marrow

text: Mus musculus

text: female

text: Sample 25


E-MTAB-5349:Sample 26

characteristic: ENA-FIRST-PUBLIC

text: ETH Zurich, Switzerland

text: public

text: E-MTAB-5349:Sample 26

text: 8 to 10

characteristic: age

text: ArrayExpress

characteristic: broker name

text: naive B cell

characteristic: cell type

text: house mouse

text: OVA mouse 4

text: Mus musculus

text: Mus musculus

text: spleen

text: Mus musculus

text: female

text: Sample 26

characteristic: title


E-MTAB-5349:Sample 30

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: ETH Zurich, Switzerland

text: public

text: E-MTAB-5349:Sample 30

text: 8 to 10

text: ArrayExpress

text: long lived plasma cell

text: house mouse

characteristic: description

text: OVA mouse 5

characteristic: individual

text: Mus musculus

text: Mus musculus

text: bone marrow

characteristic: organism part

text: Mus musculus

text: female

text: Sample 30


E-MTAB-5349:Sample 28

characteristic: External Id

text: ETH Zurich, Switzerland

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: E-MTAB-5349:Sample 28

text: 8 to 10

text: ArrayExpress

text: pre-B cell

text: house mouse

text: OVA mouse 5

text: Mus musculus

text: Mus musculus

text: bone marrow

text: Mus musculus

text: female

characteristic: sex

text: Sample 28


E-MTAB-5349:Sample 29

text: ETH Zurich, Switzerland

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-5349:Sample 29

characteristic: Submitter Id

text: 8 to 10

text: ArrayExpress

text: naive B cell

text: house mouse

characteristic: common name

text: OVA mouse 5

text: Mus musculus

text: Mus musculus

text: spleen

text: Mus musculus

text: female

characteristic: strain

text: Sample 29


characteristic: ENA-FIRST-PUBLIC

text: public

tag: Namespace:Department of Cell Biology National Cerebral and Cardiovascular Center Research Institute

text: ArrayExpress

characteristic: broker name

text: cardiac myocyte

characteristic: cell type

text: zebrafish

text: Protocols: To obtain only cardiomyocytes from the heart, two transgenic zebrafish lines were established; Tg(myl7:NLS-mCherry) and Tg(myl7:actn2-tdEos). Using cardiac myosin light chain (myl7) promoter, nuclear localization signal conjugated monomeric Cherry or actinin alpha 2 conjugated tandem Eos was expressed in cardiomyocytes. Hearts resected from Tg(myl7:NLS-mCherry) or Tg(myl7:actn2-tdEos) larvae at 72 hpf (hours post-fertilization) were collected and digested with protease solution (PBS with 5 mg/ml trypsin and 1 mM EDTA, pH 8.0) for 1 h. Digestion was terminated with stop solution (PBS with 30% fetal bovine serum and 6 mL calcium chloride). mCherry- or tdEos-positive cells were collected as cardiomyocytes using a FACS Aria III cell sorter (BD Bioscience) Total RNAs were prepared from mCherry- or tdEos-positive cells using a NucleoSpin XS kit (Macherey-Nagel) according to the manufacture’s instruction. Reverse transcription (RT) and cDNA library preparation were performed with a SMARTer Ultra Low RNA kit (Clontech). cDNA was fragmented with a Covaris S 220 instrument (Covaris). Subsequently, the sample was end-repaired, dA-tailed, adaptor ligated, and then subjected to PCR by using a NEBNext DNA Library Preparation and NEBNext Multiplex oligos for Illumina (New England BioLabs).

text: 72 hpf

characteristic: developmental stage

characteristic: genotype

text: Danio rerio

characteristic: organism

text: Danio rerio

text: Danio rerio


characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: Department of Cell Biology National Cerebral and Cardiovascular Center Research Institute

text: public

text: ArrayExpress

text: cardiac myocyte

text: zebrafish

characteristic: description

text: 72 hpf

text: Danio rerio

text: Danio rerio

text: Danio rerio

characteristic: title


text: female

characteristic: ArrayExpress-Sex

text: Homo sapiens

characteristic: ArrayExpress-Species

characteristic: External Id

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

text: human

text: Homo sapiens

text: Homo sapiens


E-MTAB-5347:Sample 1

text: University of Hawaii Cancer Center

text: public

characteristic: INSDC status

characteristic: SRA accession

text: E-MTAB-5347:Sample 1

tag: Namespace:University of Hawaii Cancer Center

characteristic: Submitter Id

text: ArrayExpress

characteristic: cell line

text: human

characteristic: common name

text: Protocols: Each group contain duplicated control samples and hDBR1 overexpressed samples (2 EV controls of Calu6, 2 hDBR1 + of Calu6; 2 EV controls of 293T, 2 hDBR1 + of 293T). RNA samples were extracted from cells by Qiagen RNeasy Plus Mini Kit. Illumina Automated RNA‐Seq

text: expression vector control

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Sample 1


E-MTAB-5347:Sample 10

characteristic: ENA-FIRST-PUBLIC

text: University of Hawaii Cancer Center

text: public

text: E-MTAB-5347:Sample 10

text: ArrayExpress

characteristic: broker name

text: human

text: expression vector control

characteristic: genotype

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Sample 10


E-MTAB-5347:Sample 11

characteristic: ENA-CHECKLIST

characteristic: ENA-LAST-UPDATE

text: University of Hawaii Cancer Center

text: public

text: E-MTAB-5347:Sample 11

text: ArrayExpress

text: human

text: expression vector control

text: Homo sapiens

characteristic: organism

text: Homo sapiens

text: Homo sapiens

text: Sample 11


E-MTAB-5347:Sample 12

text: University of Hawaii Cancer Center

text: public

text: E-MTAB-5347:Sample 12

text: ArrayExpress

text: human

characteristic: description

text: expression vector control

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Sample 12

characteristic: title


E-MTAB-5347:Sample 13

characteristic: External Id

text: University of Hawaii Cancer Center

text: public

text: E-MTAB-5347:Sample 13

text: ArrayExpress

text: human

text: hDBR1 OVEREXPRESS

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Sample 13


E-MTAB-5347:Sample 14

text: University of Hawaii Cancer Center

characteristic: INSDC center name

text: public

text: E-MTAB-5347:Sample 14

text: ArrayExpress

text: human

text: hDBR1 OVEREXPRESS

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Sample 14


E-MTAB-5347:Sample 15

text: University of Hawaii Cancer Center

characteristic: INSDC first public

text: public

text: E-MTAB-5347:Sample 15

text: ArrayExpress

text: human

text: hDBR1 OVEREXPRESS

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Sample 15


E-MTAB-5347:Sample 16

text: University of Hawaii Cancer Center

characteristic: INSDC last update

text: public

text: E-MTAB-5347:Sample 16

text: ArrayExpress

text: human

text: hDBR1 OVEREXPRESS

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Sample 16


E-MTAB-5347:Sample 2

text: University of Hawaii Cancer Center

text: public

characteristic: INSDC status

text: E-MTAB-5347:Sample 2

text: ArrayExpress

text: human

text: expression vector control

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Sample 2


E-MTAB-5347:Sample 3

text: University of Hawaii Cancer Center

text: public

characteristic: SRA accession

text: E-MTAB-5347:Sample 3

text: ArrayExpress

text: human

text: expression vector control

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Sample 3


E-MTAB-5347:Sample 4

text: University of Hawaii Cancer Center

text: public

text: E-MTAB-5347:Sample 4

characteristic: Submitter Id

text: ArrayExpress

text: human

text: expression vector control

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Sample 4


E-MTAB-5347:Sample 5

text: University of Hawaii Cancer Center

text: public

text: E-MTAB-5347:Sample 5

text: ArrayExpress

characteristic: cell line

text: human

text: hDBR1 OVEREXPRESS

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Sample 5


E-MTAB-5347:Sample 6

text: University of Hawaii Cancer Center

text: public

text: E-MTAB-5347:Sample 6

text: ArrayExpress

text: human

characteristic: common name

text: hDBR1 OVEREXPRESS

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Sample 6


E-MTAB-5347:Sample 7

characteristic: ENA-FIRST-PUBLIC

text: University of Hawaii Cancer Center

text: public

text: E-MTAB-5347:Sample 7

text: ArrayExpress

text: human

text: hDBR1 OVEREXPRESS

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Sample 7


E-MTAB-5347:Sample 8

text: University of Hawaii Cancer Center

text: public

text: E-MTAB-5347:Sample 8

text: ArrayExpress

characteristic: broker name

text: human

text: hDBR1 OVEREXPRESS

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Sample 8


E-MTAB-5347:Sample 9

text: University of Hawaii Cancer Center

text: public

text: E-MTAB-5347:Sample 9

text: ArrayExpress

text: human

text: expression vector control

characteristic: genotype

text: Homo sapiens

text: Homo sapiens

text: Homo sapiens

text: Sample 9


characteristic: ENA-CHECKLIST

text: SCILIFELAB STOCKHOLM

text: public

tag: Namespace:SCILIFELAB STOCKHOLM

text: Rhesus monkey

text: Macaca mulatta

text: Chinese

characteristic: origin

text: Macaca mulatta


characteristic: ENA-LAST-UPDATE

text: SCILIFELAB STOCKHOLM

text: public

text: Rhesus monkey

text: Macaca mulatta

characteristic: organism

text: Indian

text: Macaca mulatta


text: SCILIFELAB STOCKHOLM

text: public

text: house mouse

text: Mus musculus

text: Mus musculus

characteristic: title


text: Klebsiella pneumoniae

characteristic: ArrayExpress-Species

characteristic: External Id

text: SC

text: public

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae

text: Klebsiella pneumoniae


text: Uncultured bacterium

characteristic: ArrayExpress-StrainOrLine

text: SC

characteristic: INSDC center name

characteristic: INSDC first public

text: public

text: Uncultured bacterium

text: uncultured bacterium

text: nasopharyngeal microbiota

characteristic: sample description

text: uncultured bacterium

characteristic: strain


text: National Swine Fever Reference Center

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

tag: Namespace:National Swine Fever Reference Center

characteristic: Submitter Id

text: LETIZIA CEGLIE

characteristic: collected by

characteristic: collection date

text: To gain further insight into the genomic features of bovine viral diarrhea virus 1 (BVDV-1) subgenotypes, we sequenced the complete genome of a BVDV isolated from a persistently infected animal. The complete genome consists of 12,295 nucleotides (nt); the open reading frame comprises 11,694 nt coding 3,898 amino acids. Phylogenetic analyses of the complete coding sequence confirms that the BVDV isolate belongs to a novel sub-genotype. This is the first report of a full-length sequence of a novel BVDV-1.

characteristic: description

text: Italy

characteristic: geographic location (country and/or sea)

text: VENETO

characteristic: geographic location (region and locality)

text: BOS TAURUS

characteristic: host scientific name

text: SERUM

characteristic: isolation source

text: Bovine viral diarrhea virus 1

text: Bovine viral diarrhea virus 1

text: BLOOD

characteristic: tissue type

text: Complete genome sequence of a novel Bovine viral diarrhea virus sub-genotype isolated in Italy.


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: public

tag: Namespace:MCGILL UNIVERSITY AND GENOME QUEBEC INNOVATION CEN

text: Transcriptomic changes induced by arsenic stress in hydroponically grown Salix purpurea

text: roots

characteristic: environment (biome)

text: water

characteristic: environment (feature)

text: water

characteristic: environment (material)

text: Canada

unit: DD

characteristic: geographic location (latitude)

characteristic: geographic location (longitude)

text: metatranscriptome

characteristic: investigation type

text: Salix purpurea

characteristic: organism

text: plant-associated

characteristic: plant-associated environmental package

text: Salix purpurea roots

characteristic: project name

text: Salix purpurea

text: HiSeq

characteristic: sequencing method

text: Transcriptomics of S. purpurea roots

characteristic: title


text: MCGILL UNIVERSITY AND GENOME QUEBEC INNOVATION CEN

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: collection date

characteristic: description

text: roots

text: water

text: water

text: Canada

characteristic: geographic location (country and/or sea)

text: metatranscriptome

text: Salix purpurea

text: plant-associated

text: Salix purpurea roots

text: Salix purpurea

text: HiSeq

text: Transcriptomics of S. purpurea roots


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NEW YORK UNIVERSITY SCHOOL OF MEDICINE

text: public

tag: Namespace:NEW YORK UNIVERSITY SCHOOL OF MEDICINE

text: urban biome

characteristic: environment (biome)

text: animal-associated habitat

characteristic: environment (feature)

text: feces

characteristic: environment (material)

text: USA

characteristic: geographic location (latitude)

unit: centuries

characteristic: host age

text: Fecal

characteristic: host body habitat

characteristic: host genotype

text: female

characteristic: host sex

characteristic: host subject id

text: host-associated

characteristic: host-associated environmental package

text: metagenome

text: mouse gut metagenome

text: mouse gut metagenome

text: Illumina Hiseq

text: STAT Wild type pup

text: STAT

characteristic: treatment


text: NEW YORK UNIVERSITY SCHOOL OF MEDICINE

characteristic: INSDC center name

characteristic: INSDC first public

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text: public

characteristic: INSDC status

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text: urban biome

text: animal-associated habitat

text: feces

text: USA

characteristic: geographic location (longitude)

text: Fecal

text: female

text: host-associated

text: metagenome

characteristic: investigation type

text: mouse gut metagenome

characteristic: organism

characteristic: project name

text: mouse gut metagenome

text: Illumina Hiseq

characteristic: sequencing method

text: Control Wild-type pup

characteristic: title

text: Control


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: NEW YORK UNIVERSITY SCHOOL OF MEDICINE

text: public

text: urban biome

characteristic: environment (biome)

text: animal-associated habitat

characteristic: environment (feature)

text: feces

characteristic: environment (material)

text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: host age

text: Fecal

characteristic: host body habitat

characteristic: host genotype

text: female

characteristic: host sex

characteristic: host subject id

text: host-associated

text: metagenome

text: mouse gut metagenome

text: mouse gut metagenome

text: Illumina Hiseq

text: Control-IL10 pup

text: Control


text: NEW YORK UNIVERSITY SCHOOL OF MEDICINE

characteristic: INSDC center name

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text: public

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text: urban biome

text: animal-associated habitat

text: feces

text: USA

characteristic: geographic location (longitude)

text: incolumn

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text: metagenome

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text: mouse gut metagenome

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text: mouse gut metagenome

text: Illumina Hiseq

text: STAT-inoculum

text: STAT

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characteristic: External Id

text: NEW YORK UNIVERSITY SCHOOL OF MEDICINE

text: public

text: urban biome

characteristic: environment (biome)

text: animal-associated habitat

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text: feces

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text: USA

characteristic: geographic location (country and/or sea)

characteristic: geographic location (latitude)

characteristic: host age

text: incolumn

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text: host-associated

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text: Illumina Hiseq

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text: Control-inoculum

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text: Control


text: NEW YORK UNIVERSITY SCHOOL OF MEDICINE

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text: public

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text: urban biome

text: animal-associated habitat

text: feces

text: USA

characteristic: geographic location (longitude)

text: Fecal

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text: female

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text: host-associated

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text: metagenome

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text: mouse gut metagenome

text: mouse gut metagenome

text: Illumina Hiseq

text: STAT-IL10 pup

text: STAT


characteristic: ENA-CHECKLIST

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characteristic: External Id

text: UCSDMI

text: public

text: Adult

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text: UBERON:feces

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text: UBERON:feces

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text: UBERON:feces

characteristic: body site

text: howler money fecal sample

text: TRUE

characteristic: elevation

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text: mixed forest biome

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text: animal-associated habitat

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text: host-associated

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text: feces

text: Mexico

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text: Alouatta pigra

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text: Luz

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text: primate metagenome

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text: Northwestern University

text: TRUE

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text: stool

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characteristic: sloan

text: Balam

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characteristic: External Id

text: UCSDMI

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text: public

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text: UBERON:feces

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text: howler money fecal sample

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text: TRUE

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text: Milo

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text: howler money fecal sample

text: TRUE

characteristic: elevation

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text: primate metagenome

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text: UCSDMI

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text: public

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text: Juvenile

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text: howler money fecal sample

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text: Rufus

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text: howler money fecal sample

text: TRUE

characteristic: elevation

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characteristic: ENA-CHECKLIST

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text: UCSDMI

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characteristic: title


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text: howler money fecal sample

text: TRUE

characteristic: elevation

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text: mixed forest biome

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text: primate metagenome

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text: Northwestern University

text: TRUE

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text: Motiepa

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text: UCSDMI

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text: mixed forest biome

text: animal-associated habitat

text: host-associated

text: feces

text: Mexico

text: Alouatta pigra

text: Dinka

characteristic: host subject id

text: primate metagenome

text: Northwestern University

text: TRUE

text: stool

text: primate metagenome

text: female

text: Bolas

characteristic: title


characteristic: ENA-CHECKLIST

text: UCSDMI

text: public

text: Adult

text: UBERON:feces

text: UBERON:feces

text: UBERON:feces

text: howler money fecal sample

text: TRUE

characteristic: elevation

characteristic: emp status

text: mixed forest biome

characteristic: env biome

text: animal-associated habitat

characteristic: env feature

text: host-associated

characteristic: env package

text: feces

text: Mexico

characteristic: geo loc name

text: Alouatta pigra

characteristic: host scientific name

text: Puma

characteristic: host taxid

characteristic: latitude

characteristic: longitude

text: primate metagenome

characteristic: organism

text: Northwestern University

text: TRUE

characteristic: plate

text: stool

characteristic: sample type

text: primate metagenome

text: female

characteristic: sex

characteristic: sloan

text: Bolas

characteristic: social group


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Adult

characteristic: age

text: UBERON:feces

characteristic: body habitat

text: UBERON:feces

characteristic: body product

text: UBERON:feces

characteristic: body site

text: howler money fecal sample

characteristic: description

text: TRUE

text: mixed forest biome

text: animal-associated habitat

text: host-associated

text: feces

text: Mexico

text: Alouatta pigra

text: Boquinche

characteristic: host subject id

text: primate metagenome

text: Northwestern University

text: TRUE

text: stool

text: primate metagenome

text: male

text: Unitestes

characteristic: title


characteristic: ENA-CHECKLIST

text: UCSDMI

text: public

text: Adult

text: UBERON:feces

text: UBERON:feces

text: UBERON:feces

text: howler money fecal sample

text: TRUE

characteristic: elevation

characteristic: emp status

text: mixed forest biome

characteristic: env biome

text: animal-associated habitat

characteristic: env feature

text: host-associated

characteristic: env package

text: feces

text: Mexico

characteristic: geo loc name

text: Alouatta pigra

characteristic: host scientific name

text: Dinde

characteristic: host taxid

characteristic: latitude

characteristic: longitude

text: primate metagenome

characteristic: organism

text: Northwestern University

text: TRUE

characteristic: plate

text: stool

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text: primate metagenome

text: male

characteristic: sex

characteristic: sloan

text: Unitestes

characteristic: social group


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Sub

characteristic: age

text: UBERON:feces

characteristic: body habitat

text: UBERON:feces

characteristic: body product

text: UBERON:feces

characteristic: body site

text: howler money fecal sample

characteristic: description

text: TRUE

text: mixed forest biome

text: animal-associated habitat

text: host-associated

text: feces

text: Mexico

text: Alouatta pigra

text: Luigi

characteristic: host subject id

text: primate metagenome

text: Northwestern University

text: TRUE

text: stool

text: primate metagenome

text: male

text: Unitestes

characteristic: title


characteristic: ENA-CHECKLIST

text: UCSDMI

text: public

text: Juvenile

text: UBERON:feces

text: UBERON:feces

text: UBERON:feces

text: howler money fecal sample

text: TRUE

characteristic: elevation

characteristic: emp status

text: mixed forest biome

characteristic: env biome

text: animal-associated habitat

characteristic: env feature

text: host-associated

characteristic: env package

text: feces

text: Mexico

characteristic: geo loc name

text: Alouatta pigra

characteristic: host scientific name

text: Copal

characteristic: host taxid

characteristic: latitude

characteristic: longitude

text: primate metagenome

characteristic: organism

text: Northwestern University

text: TRUE

characteristic: plate

text: stool

characteristic: sample type

text: primate metagenome

text: male

characteristic: sex

characteristic: sloan

text: Unitestes

characteristic: social group


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Adult

characteristic: age

text: UBERON:feces

characteristic: body habitat

text: UBERON:feces

characteristic: body product

text: UBERON:feces

characteristic: body site

text: howler money fecal sample

characteristic: description

text: TRUE

text: mixed forest biome

text: animal-associated habitat

text: host-associated

text: feces

text: Mexico

text: Alouatta pigra

text: Pura

characteristic: host subject id

text: primate metagenome

text: Northwestern University

text: TRUE

text: stool

text: primate metagenome

text: female

text: Pakal

characteristic: title


characteristic: ENA-CHECKLIST

text: UCSDMI

text: public

text: Adult

text: UBERON:feces

text: UBERON:feces

text: UBERON:feces

text: howler money fecal sample

text: TRUE

characteristic: elevation

characteristic: emp status

text: mixed forest biome

characteristic: env biome

text: animal-associated habitat

characteristic: env feature

text: host-associated

characteristic: env package

text: feces

text: Mexico

characteristic: geo loc name

text: Alouatta pigra

characteristic: host scientific name

text: Pakal

characteristic: host taxid

characteristic: latitude

characteristic: longitude

text: primate metagenome

characteristic: organism

text: Northwestern University

text: TRUE

characteristic: plate

text: stool

characteristic: sample type

text: primate metagenome

text: male

characteristic: sex

characteristic: sloan

text: Pakal

characteristic: social group


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Adult

characteristic: age

text: UBERON:feces

characteristic: body habitat

text: UBERON:feces

characteristic: body product

text: UBERON:feces

characteristic: body site

text: howler money fecal sample

characteristic: description

text: TRUE

text: mixed forest biome

text: animal-associated habitat

text: host-associated

text: feces

text: Mexico

text: Alouatta pigra

text: Esma

characteristic: host subject id

text: primate metagenome

text: Northwestern University

text: TRUE

text: stool

text: primate metagenome

text: female

text: Pakal

characteristic: title


characteristic: ENA-CHECKLIST

text: UCSDMI

text: public

text: Sub

text: UBERON:feces

text: UBERON:feces

text: UBERON:feces

text: howler money fecal sample

text: TRUE

characteristic: elevation

characteristic: emp status

text: mixed forest biome

characteristic: env biome

text: animal-associated habitat

characteristic: env feature

text: host-associated

characteristic: env package

text: feces

text: Mexico

characteristic: geo loc name

text: Alouatta pigra

characteristic: host scientific name

text: Kanika

characteristic: host taxid

characteristic: latitude

characteristic: longitude

text: primate metagenome

characteristic: organism

text: Northwestern University

text: TRUE

characteristic: plate

text: stool

characteristic: sample type

text: primate metagenome

text: female

characteristic: sex

characteristic: sloan

text: Pakal

characteristic: social group


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

text: Adult

characteristic: age

text: UBERON:feces

characteristic: body habitat

text: UBERON:feces

characteristic: body product

text: UBERON:feces

characteristic: body site

text: howler money fecal sample

characteristic: description

text: TRUE

text: mixed forest biome

text: animal-associated habitat

text: host-associated

text: feces

text: Mexico

text: Alouatta pigra

text: Kin

characteristic: host subject id

text: primate metagenome

text: Northwestern University

text: TRUE

text: stool

text: primate metagenome

text: male

text: Pakal

characteristic: title


characteristic: ENA-CHECKLIST

text: UCSDMI

text: public

text: Adult

text: UBERON:feces

text: UBERON:feces

text: UBERON:feces

text: howler money fecal sample

text: TRUE

characteristic: elevation

characteristic: emp status

text: mixed forest biome

characteristic: env biome

text: animal-associated habitat

characteristic: env feature

text: host-associated

characteristic: env package

text: feces

text: Mexico

characteristic: geo loc name

text: Alouatta pigra

characteristic: host scientific name

text: Parcha

characteristic: host taxid

characteristic: latitude

characteristic: longitude

text: primate metagenome

characteristic: organism

text: Northwestern University

text: TRUE

characteristic: plate

text: stool

characteristic: sample type

text: primate metagenome

text: female

characteristic: sex

characteristic: sloan

text: Pakal

characteristic: social group


characteristic: ENA-CHECKLIST

characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: anonymized name

characteristic: depth

text: 1226D1H5 Deeply buried marine sediment

characteristic: description

text: TRUE

text: marine benthic biome

text: ocean floor

text: sediment

text: marine sediment

text: TRUE

characteristic: extracted DNA avail now

text: Pacific Ocean

characteristic: host subject id

text: marine sediment metagenome

text: LBNL

text: FALSE

text: TRUE

characteristic: public

text: completed

text: marine sediment

text: sequenced (16S)

text: marine sediment metagenome

text: Ocean Drilling Program Leg 201

characteristic: title


characteristic: ENA-CHECKLIST

text: UCSDMI

text: public

text: 1226D2H3 Deeply buried marine sediment

text: TRUE

characteristic: elevation

text: marine benthic biome

characteristic: env biome

text: ocean floor

characteristic: env feature

text: sediment

characteristic: env package

text: marine sediment

text: TRUE

text: Pacific Ocean

characteristic: geo loc name

characteristic: latitude

characteristic: longitude

text: marine sediment metagenome

characteristic: organism

text: LBNL

text: FALSE

text: TRUE

text: completed

text: marine sediment

characteristic: sample type

text: sequenced (16S)

text: marine sediment metagenome

text: Ocean Drilling Program Leg 201


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: anonymized name

text: 1230A1H1 Deeply buried marine sediment

text: TRUE

text: marine benthic biome

text: ocean floor

text: sediment

text: marine sediment

text: TRUE

text: Pacific Ocean

text: marine sediment metagenome

text: LBNL

text: FALSE

text: TRUE

text: completed

text: marine sediment

text: sequenced (16S)

text: marine sediment metagenome

text: Ocean Drilling Program Leg 201


characteristic: ENA-CHECKLIST

text: UCSDMI

text: public

characteristic: depth

text: 1230A2H2 Deeply buried marine sediment

characteristic: description

text: TRUE

characteristic: elevation

text: marine benthic biome

characteristic: env biome

text: ocean floor

characteristic: env feature

text: sediment

text: marine sediment

text: TRUE

characteristic: extracted DNA avail now

text: Pacific Ocean

characteristic: host subject id

text: marine sediment metagenome

text: LBNL

text: FALSE

text: TRUE

characteristic: public

text: completed

text: marine sediment

text: sequenced (16S)

text: marine sediment metagenome

text: Ocean Drilling Program Leg 201

characteristic: title


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

text: public

text: 1230C2H2 Deeply buried marine sediment

text: TRUE

text: marine benthic biome

text: ocean floor

text: sediment

characteristic: env package

text: marine sediment

text: TRUE

text: Pacific Ocean

characteristic: geo loc name

characteristic: latitude

characteristic: longitude

text: marine sediment metagenome

characteristic: organism

text: LBNL

text: FALSE

text: TRUE

text: completed

text: marine sediment

characteristic: sample type

text: sequenced (16S)

text: marine sediment metagenome

text: Ocean Drilling Program Leg 201


characteristic: ENA-CHECKLIST

text: UCSDMI

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

characteristic: SRA accession

characteristic: Submitter Id

characteristic: anonymized name

characteristic: depth

text: 1230C2H3 Deeply buried marine sediment

characteristic: description

text: TRUE

characteristic: elevation

text: marine benthic biome

text: ocean floor

text: sediment

text: marine sediment

text: TRUE

text: Pacific Ocean

text: marine sediment metagenome

text: LBNL

text: FALSE

text: TRUE

text: completed

text: marine sediment

text: sequenced (16S)

text: marine sediment metagenome

text: Ocean Drilling Program Leg 201


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

text: public

text: 1230C2H4 Deeply buried marine sediment

text: TRUE

text: marine benthic biome

characteristic: env biome

text: ocean floor

characteristic: env feature

text: sediment

text: marine sediment

text: TRUE

characteristic: extracted DNA avail now

text: Pacific Ocean

characteristic: host subject id

text: marine sediment metagenome

text: LBNL

text: FALSE

text: TRUE

characteristic: public

text: completed

text: marine sediment

text: sequenced (16S)

text: marine sediment metagenome

text: Ocean Drilling Program Leg 201

characteristic: title


characteristic: ENA-CHECKLIST

text: UCSDMI

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: maize

characteristic: crop

text: crop

text: Soil from maize field tilled by ripper subsoiling (max depth 40cm)

text: TRUE

text: cropland biome

text: agricultural feature

text: soil

characteristic: env package

text: agricultural soil

text: Italy:Tuscany:Fagna

characteristic: geo loc name

characteristic: latitude

text: Fagna, Tuscany

characteristic: location

characteristic: longitude

text: soil metagenome

characteristic: organism

text: Missing: Not provided

characteristic: ph

text: Missing: Not provided

characteristic: ph method

text: LBNL

text: FALSE

text: ripper subsoiling

text: TRUE

text: completed

text: soil

characteristic: sample type

text: Sequenced (16S)

text: soil metagenome

text: ripper subsoiling

text: Influence of tillage practices on soil microbial diversity and activity in a long-term corn experimental field under continuous maize production

text: Missing: Not provided

text: Missing: Not provided

text: Missing: Not provided


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: anonymized name

text: maize

text: crop

characteristic: depth

characteristic: description

text: TRUE

characteristic: elevation

text: cropland biome

characteristic: env biome

text: agricultural feature

characteristic: env feature

text: soil

text: agricultural soil

text: Italy:Tuscany:Fagna

characteristic: host subject id

text: Fagna, Tuscany

text: soil metagenome

text: Missing: Not provided

text: Missing: Not provided

text: LBNL

text: FALSE

text: ripper subsoiling

text: TRUE

text: completed

text: soil

text: Sequenced (16S)

text: soil metagenome

text: ripper subsoiling

text: Missing: Not provided

text: Missing: Not provided

text: Missing: Not provided


characteristic: ENA-CHECKLIST

text: UCSDMI

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: maize

characteristic: crop

text: crop

text: Soil from maize field tilled by conventional deep ploughing (max depth 40cm)

text: TRUE

text: cropland biome

text: agricultural feature

text: soil

characteristic: env package

text: agricultural soil

text: Italy:Tuscany:Fagna

characteristic: geo loc name

characteristic: latitude

text: Fagna, Tuscany

characteristic: location

characteristic: longitude

text: soil metagenome

characteristic: organism

text: Missing: Not provided

text: Missing: Not provided

text: LBNL

text: FALSE

text: conventional

text: TRUE

characteristic: public

text: completed

text: soil

text: Sequenced (16S)

text: soil metagenome

text: deep ploughing

characteristic: title

text: Missing: Not provided

text: Missing: Not provided

text: Missing: Not provided


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

text: public

characteristic: SRA accession

characteristic: Submitter Id

characteristic: anonymized name

text: maize

text: crop

characteristic: depth

text: Soil from maize field tilled by mouldboard ploughing (max depth 20cm)

characteristic: description

text: TRUE

characteristic: elevation

text: cropland biome

text: agricultural feature

text: soil

text: agricultural soil

text: Italy:Tuscany:Fagna

text: Fagna, Tuscany

text: soil metagenome

text: Missing: Not provided

characteristic: ph

text: Missing: Not provided

characteristic: ph method

text: LBNL

text: FALSE

text: mouldboard

text: TRUE

text: completed

text: soil

characteristic: sample type

text: Sequenced (16S)

text: soil metagenome

text: shallow ploughing

text: Missing: Not provided

text: Missing: Not provided

text: Missing: Not provided


characteristic: ENA-CHECKLIST

text: UCSDMI

characteristic: INSDC first public

characteristic: INSDC last update

text: public

characteristic: INSDC status

text: maize

characteristic: crop

text: crop

text: Soil from maize field tilled by harrowing with a disk (minimum tillage)

text: TRUE

text: cropland biome

characteristic: env biome

text: agricultural feature

characteristic: env feature

text: soil

characteristic: env package

text: agricultural soil

text: Italy:Tuscany:Fagna

characteristic: geo loc name

characteristic: host subject id

characteristic: latitude

text: Fagna, Tuscany

characteristic: location

characteristic: longitude

text: soil metagenome

text: Missing: Not provided

text: Missing: Not provided

text: LBNL

text: FALSE

text: harrowing with disk

text: TRUE

text: completed

text: soil

text: Sequenced (16S)

text: soil metagenome

text: minimum tillage

text: Missing: Not provided

text: Missing: Not provided

text: Missing: Not provided


characteristic: ENA-FIRST-PUBLIC

characteristic: ENA-LAST-UPDATE

characteristic: External Id

text: UCSDMI

characteristic: INSDC center name

text: public

characteristic: SRA accession

text: qiita_sid_808:808.AK.19.12a.s.4.1.sequences

characteristic: Submitter Id

characteristic: anonymized name

characteristic: depth

text: soil core NEON AK

characteristic: description

text: True

characteristic: elevation

text: forest biome

text: taiga

text: ENVO:soil

characteristic: env matter

text: soil

text: soil

text: TRUE

characteristic: extracted DNA avail now

text: USA:AK

text: A horizon

characteristic: horizon

text: Histic Pergelic Cryaquepts

text: soil metagenome

characteristic: organism

text: ANL

text: FALSE

text: TRUE

characteristic: public

text: completed

text: soil

text: Sequenced (16S)

text: soil metagenome

text: Taiga Domain 19, Alaska

characteristic: site

characteristic: title

text: Missing: Not provided